gebhart/patrepeval huggingface dataset:
Mechano-regulated surface for manipulating liquid droplets (2017)

The effective transfer of tiny liquid droplets is vital for a number of processes such as chemical and biological microassays. Inspired by the tarsi of meniscus-climbing insects, which can climb menisci by deforming the water/air interface, we developed a mechano-regulated surface consisting of a background mesh and a movable microfibre array with contrastive wettability. The adhesion of this mechano-regulated surface to liquid droplets can be reversibly switched through mechanical reconfiguration of the microfibre array. The adhesive force can be tuned by varying the number and surface chemistry of the microfibres. The in situ adhesion of the mechano-regulated surface can be used to manoeuvre micro-/nanolitre liquid droplets in a nearly loss-free manner. The mechano-regulated surface can be scaled up to handle multiple droplets in parallel. Our approach offers a miniaturized mechano-device with switchable adhesion for handling micro-/nanolitre droplets, either in air or in a fluid that is immiscible with the droplets.
Combinatorial Templates Generated by Dip-Pen Nanolithography for the Formation of Two-Dimensional Particle Arrays (2001)

Lattices of single polystyrene particles were constructed by using a combinatorial approach to analyze particle pattern recognition properties. Dip-pen nanolithography was used to generate chemical templates of 16-thiohexadecanoic acid on a gold surface to study the two-dimensional assembly of amine- and amidine-modified particles.
Maitake extracts and their therapeutic potential. (2001)

Maitake (Grifola frondosa) is the Japanese name for an edible fungus with a large fruiting body characterized by overlapping caps. It is a premier culinary as well as medicinal mushroom. Maitake is increasingly being recognized as a potent source of polysaccharide compounds with dramatic health-promoting potential. The most recent development is the MD-fraction, a proprietary maitake extract its Japanese inventors consider to be a notable advance upon the preceding D-fraction. The D-fraction, the MD-fraction, and other extracts, often in combination with whole maitake powder, have shown particular promise as immunomodulating agents, and as an adjunct to cancer and HIV therapy. They may also provide some benefit in the treatment of hyperlipidemia, hypertension, and hepatitis.
Auditory Wavelet Transform Based On Auditory Wavelet Families (2006)

The auditory periphery system receives a one dimensional acoustical signal that describes how the local pressure varies with time. However, this one dimensional signal information is then somehow unfolded into a two dimensional time-frequency plane, that tells us when which frequency occurs. The hearing process is based on compromise between time localization and frequency localization. A kind of time-frequency or wavelet type transformation is done in auditory signal processing. In this study the similarities between auditory transform based on the auditory physiological process and wavelet transform are introduced. Specially, band pass filter bank properties and variable time and frequency resolutions with the signal frequency are considered. The main goal is to find the scaling function while the numerical values of the wavelet function were measured. If the wavelet function and the scaling function from the measured data are estimated, then the wavelet coefficients and the scaling coefficients could be calculated. Therefore, the multiresolution implementation of auditory based wavelet transform is possible.
Learning sparse doubly-selective channels (2008)

Coherent data communication over doubly-selective channels requires that the channel response be known at the receiver. Training-based schemes, which involve probing of the channel with known signaling waveforms and processing of the corresponding channel output to estimate the channel parameters, are commonly employed to learn the channel response in practice. Conventional training-based methods, often comprising of linear least squares channel estimators, are known to be optimal under the assumption of rich multipath channels. Numerous measurement campaigns have shown, however, that physical multipath channels tend to exhibit a sparse structure at high signal space dimension (time-bandwidth product), and can be characterized with significantly fewer parameters compared to the maximum number dictated by the delay-Doppler spread of the channel. In this paper, it is established that traditional training-based channel learning techniques are ill-suited to fully exploiting the inherent low-dimensionality of sparse channels. In contrast, key ideas from the emerging theory of compressed sensing are leveraged to propose sparse channel learning methods for both single-carrier and multicarrier probing waveforms that employ reconstruction algorithms based on convex/linear programming. In particular, it is shown that the performance of the proposed schemes come within a logarithmic factor of that of an ideal channel estimator, leading to significant reductions in the training energy and the loss in spectral efficiency associated with conventional training-based methods.
Interactive surface modeling using modal analysis (2011)

We propose a framework for deformation-based surface modeling that is interactive, robust, and intuitive to use. The deformations are described by a nonlinear optimization problem that models static states of elastic shapes under external forces which implement the user input. Interactive response is achieved by a combination of model reduction, a robust energy approximation, and an efficient quasi-Newton solver. Motivated by the observation that a typical modeling session requires only a fraction of the full shape space of the underlying model, we use second and third derivatives of a deformation energy to construct a low-dimensional shape space that forms the feasible set for the optimization. Based on mesh coarsening, we propose an energy approximation scheme with adjustable approximation quality. The quasi-Newton solver guarantees superlinear convergence without the need of costly Hessian evaluations during modeling. We demonstrate the effectiveness of the approach on different examples including the test suite introduced in Sorkine [2008].
Baculovirus-mediated expression of the human vitamin D receptor. Functional characterization, vitamin D response element interactions, and evidence for a receptor auxiliary factor. (1991)

A baculovirus expression vector system (BEVS) was used to overproduce the full-length human vitamin D receptor (hVDR) in Spodoptera frugiperda ovarian cells. hVDR was expressed to a level of 0.5% of the total soluble protein in this system. Western analysis demonstrated that the baculovirus-generated protein had electrophoretic and immunologic properties equivalent to those of hVDR expressed in mammalian cells. The BEVS-derived receptor displayed specificity and high affinity (apparent Kd = 0.7 nM) for the 1,25(OH)2D3 ligand. Recombinant hVDR generated a specific protein-DNA complex with a duplex oligomer containing a vitamin D-responsive element (VDRE) in gel mobility shift assays. The intensity of the VDR.VDRE complex was not affected by 1,25(OH)2D3. However, the complex exhibited increased mobility in the presence of hormone, possibly the result of a 1,25(OH)2D3-dependent conformational change. A nuclear extract obtained from CV-1 cells markedly enhanced the intensity of this VDR.VDRE complex and produced an additional distinct VDR-dependent complex, thus implicating a role for nuclear auxiliary factors in multiple high affinity VDR.VDRE interactions. Finally, methylation interference studies defined the guanine residues contacted when the putative VDR-auxiliary factor complex associates with the rat osteocalcin VDRE; specifically, all of the GC base pairs in the sequence GGGTGAATGAGGACA. Therefore, these results show that the BEV system elicits high level expression of hVDR with critical functional characteristics being preserved.
The Effect of Gamma-Hydroxybutyrate on Nocturnal and Diurnal Sleep of Normal Subjects: Further Considerations on REM Sleep-Triggering Mechanisms (1990)

Gamma-hydroxybutyrate (GHB) is a drug currently used to treat narcolepsy. The present study documents its effect on sleep organization in healthy subjects. GHB and a placebo were given at bedtime and before a morning nap in a double-blind fashion. GHB administered before nocturnal or diurnal sleep increases stages 3 and 4 and decreases stage 1 non-rapid eye movement (NREM) sleep. In addition, GHB improves REM efficiency at night and reduces wake time after sleep onset when administered before a morning nap recording. GHB also slightly decreases REM latency when administered in the morning, and this effect is correlated with age. Hypotheses regarding mechanisms of action GHB and the involvement of hypothalamic structures in the regulation of REM sleep are discussed.
Restoration of thermographic images using iterative inverse filtering (laser surgery application) (2003)

The use of the image restoration technique of iterative inverse filtering to enhance recorded thermal images is discussed. Potential problems encountered in thermographic imaging are discussed. It is shown how the camera point spread function and slit response functions can be determined. Evaluation of the technique using a calibrated aluminum target is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Significance of graft tension in anterior cruciate ligament reconstruction (1998)

The purpose of this paper is to review scientific evidence that graft tension affects remodelling of the autograft in ligament reconstruction. The in situ freezing model of the patellar tendon, an ideal patellar tendon autograft model, demonstrated that subsequent cellular proliferation following fibroblast necrosis reduces the mechanical properties of the autograft. Stress shielding enhances reduction of the strength in the once-frozen patellar tendon. The strength of the patellar tendon also changes depending on the degree of stress shielding. Transmission electron micrographs revealed that the number of small-diameter fibrils decreases in the stress-shielded tendons compared with non-stress-shielded tendons after in situ freezing. Restressing essentially restores the mechanical properties of patellar tendon autografts even if the strength has been much reduced by complete stress shielding. The effects of restressing may depend on the period of stress shielding applied before restressing. Unphysiologically high tension significantly reduces the mechanical properties of the in situ frozen anterior cruciate ligament (ACL). Therefore, not only stress-deprivation but also stress-enhancement significantly affect the mechanical properties of tendon autografts. Results of in vivo and in vitro studies suggest that cyclic tensile loading may inhibit the deterioration in mechanical strength of the transplanted tendon. Clinically, our prospective randomized study demonstrated that a relatively high initial tension reduces the postoperative anterior laxity of the knee joint after ACL reconstruction using the doubled autogenous hamstring tendons connected in series with polyester tapes, when the tension applied is less than 80 N. Our experimental and clinical results indicate that the initial tension is one of the significant factors that affect the results of ACL reconstruction, although the optimal initial tension for the other graft materials still remains unknown.
The D-basis Algorithm for Association Rules of High Confidence (2018)

We develop a new approach for distributed computing of the association rules of high confidence on the attributes/columns of a binary table. It is derived from the D-basis algorithm developed by K.Adaricheva and J.B.Nation (Theoretical Computer Science, 2017), which runs multiple times on sub-tables of a given binary table, obtained by removing one or more rows. The sets of rules retrieved at these runs are then aggregated. This allows us to obtain a basis of association rules of high confidence, which can be used for ranking all attributes of the table with respect to a given fixed attribute. This paper focuses on some algorithmic details and the technical implementation of the new algorithm. Results are given for tests performed on random, synthetic and real data.
Engineering of antibodies (1988)

Abstract Human monoclonal antibodies are extremely difficult to obtain by hybridoma technology. As an alternative, ‘human‐like’ antibodies have been produced by recombinant DNA technology. The first such engineered antibodies consisted of chimaeric proteins, in which murine variable regions were linked to human constant regions. More recently ‘human’ antibodies have been ‘reshaped’ by transplanting the binding site of a murine antibody into a human antibody. Further‐more antibodies have been dissected into groups of domains (Fab's, Fc's) and for example, Fab's have been joined to enzymes resulting in antibody‐enzyme chimaeras. In addition to their potential clinical applications, engineered antibodies will prove invaluable in studies of structure/function relationships of variable and constant regions.
Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses (2005)

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2 influenza A viruses collected between 1999 and 2004 from New York State, United States, and observed multiple co-circulating clades with different population frequencies. Strikingly, phylogenies inferred for individual gene segments revealed that multiple reassortment events had occurred among these clades, such that one clade of H3N2 viruses present at least since 2000 had provided the hemagglutinin gene for all those H3N2 viruses sampled after the 2002-2003 influenza season. This reassortment event was the likely progenitor of the antigenically variant influenza strains that caused the A/Fujian/411/2002-like epidemic of the 2003-2004 influenza season. However, despite sharing the same hemagglutinin, these phylogenetically distinct lineages of viruses continue to co-circulate in the same population. These data, derived from the first large-scale analysis of H3N2 viruses, convincingly demonstrate that multiple lineages can co-circulate, persist, and reassort in epidemiologically significant ways, and underscore the importance of genomic analyses for future influenza surveillance.
Production of helper‐dependent adenovirus vector relies on helper virus structure and complementing (2002)

Although the E3 region is not essential for adenovirus replication in vivo, deletion of this region attenuates virus replication. Production of HD-Ad vector can be further improved by modifications in helper virus structure.
Synthetic Surfactant Based on Analogues of SP-B and SP-C Is Superior to Single-Peptide Surfactants in Ventilated Premature Rabbits (2010)

The data indicate that synthetic surfactants containing analogues of both SP-B and SP-C might be superior to single-peptide surfactants in the treatment of RDS.
In vitro investigations of tumor targeting with (99m)Tc-labeled antisense DNA. (2001)

This evidence suggests tumor cell accumulation by an antisense mechanism. Moreover, the high level of DNA accumulation suggests that a rapid target mRNA turnover or transcription rate may be an important determinant of tumor counting rates.
Progress towards glucagon receptor antagonist therapy for Type 2 diabetes (2005)

Peptidal glucagon receptor antagonists and antiglucagon monoclonal antibodies lower glucose levels in diabetic rodent models, suggesting a potential to treat hyperglycaemia in Type 2 diabetics through the inhibition of glucagon function. Several research groups have discovered small molecule glucagon antagonists from multiple chemical series and at least one has been clinically evaluated. Although multiple compounds have blocked the rise in blood glucose levels in response to a glucagon challenge, no preclinical or clinical efficacy data from chronic studies have been reported. In general, drug candidate potency, pharmacokinetics, physical properties and cross-species potency have hindered progress and preclinical efficacy assessment. Recently, antisense oligonucleotides against the glucagon receptor have been described, providing a guiding post for the type of activity a small molecule glucagon antagonist may possess, as well as offering a potential therapeutic strategy.
An evaluation of XML-RPC (2003)

This paper explores the complexity and performance of the XML-RPC system for remote method invocation. We developed a program that can use either XML-RPC-based network communication or a hand-rolled version of networking code based on the java.net package. We first compare our two implementations using traditional object-oriented metrics. In addition, we conduct tests over a local network and the Internet to assess the performance of the two versions of the networking code using traditional internetworking metrics. We find that XML-RPC reduces the programming complexity of the software by roughly 50% (across various metrics). On the other hand, the hand-rolled java.net-based implementation offers up to an order of magnitude better network performance in some of our tests.
Address supply chain visibility from knowledge management perspective (2008)

Supply chain visibility is an important issue for both academic research and industrial practice. This paper presents a new insight to supply chain visibility for better collaborative decision making from knowledge management perspective. It presents a framework for managing knowledge required for collaborative decisions in supply chain processes. The framework tackles two kinds of knowledge: quantitative knowledge and qualitative knowledge. Quantitative knowledge is discovered from a supply chainpsilas business databases through data mining, and qualitative knowledge like experience and judgment captured from human in the collaborative decision making process. The infusion of quantitative and qualitative knowledge provides better understanding of supply chain processes and the decision contexts for decision makers to make decisions quickly under the pressures of time- and knowledge-based competition. The knowledge is then codified into supply chainpsilas knowledge repository. A knowledge mapping mechanism is also provided in the framework to present visibility to all partners in a supply chain by accessing knowledge from knowledge repository efficiently and effectively at anytime and anywhere.
Clinical Evaluation of an Acellular Allograft Dermal Matrix in Full-Thickness Burns (1996)

A multicenter clinical study assessed the ability of an acellular allograft dermal matrix to function as a permanent dermal transplant in full-thickness and deep partial-thickness burns. The study consisted of a pilot phase (24 patients) to identify the optimum protocol and a study phase (43 patients) to evaluate graft performance. Each patient had both a test and a mirror-image or contiguous control site. At the test site, the dermal matrix was grafted to the excised wound base and a split-thickness autograft was simultaneously applied over it. The control site was grafted with a split-thickness autograft alone. Fourteen-day take rates of the dermal matrix were statistically equivalent to the control autografts. Histology of the dermal matrix showed fibroblast infiltration, neovascularization, and neoepithelialization without evidence of rejection. Wound assessment over time showed that thin split-thickness autografts plus allograft dermal matrix were equivalent to thicker split-thickness autografts.
Phosphorescence of protein-bound eosin and erythrosin. A possible probe for measurements of slow rotational mobility (1979)

We used a pulsed dye laser working at 540 nm to excite triplet-state formation of eosin and erythrosin, either bound or unbound to bovine serum albumin, in aqueous solution anaerobically at pH 8 and 20-22 degrees C. Delayed emission from radiative transitions of the triplet state was readily detectable, both as delayed fluorescence and as red phosphorescence. Detection of the triplet state by measurement of phosphorescence at 645 nm upwards was at least 100-fold more sensitive than by absorbance measurements of ground-state depletion at 500 nm. When immobilized in poly(methyl methacrylate), the phosphorescence of eosin and erythrosin was polarized with an anisotropy parameter [Jablonski (1961) Z. Naturforsch. A16, 1-4] of about 0.25. The phosphorescence of erythrosin is sufficiently intense to be distinguishable from the long-wavelength end of fluorescence under conditions of continuous rather than pulsed excitation. Our observations suggest that phosphorescence depolarization of eosin or erythrosin probes could be used as a highly sensitive method of measuring rotational relaxation times in region from 10(-5) to 10(-3) s, such as those of the uniaxial rotation of membrane proteins.
Addressing the Global Burden of Trauma in Major Surgery (2015)

Despite a technically perfect procedure, surgical stress can determine the success or failure of an operation. Surgical trauma is often referred to as the "neglected step-child" of global health in terms of patient numbers, mortality, morbidity, and costs. A staggering 234 million major surgeries are performed every year, and depending upon country and institution, up to 4% of patients will die before leaving hospital, up to 15% will have serious post-operative morbidity, and 5-15% will be readmitted within 30 days. These percentages equate to around 1000 deaths and 4000 major complications every hour, and it has been estimated that 50% may be preventable. New frontline drugs are urgently required to make major surgery safer for the patient and more predictable for the surgeon. We review the basic physiology of the stress response from neuroendocrine to genomic systems, and discuss the paucity of clinical data supporting the use of statins, beta-adrenergic blockers and calcium-channel blockers. Since cardiac-related complications are the most common, particularly in the elderly, a key strategy would be to improve ventricular-arterial coupling to safeguard the endothelium and maintain tissue oxygenation. Reduced O2 supply is associated with glycocalyx shedding, decreased endothelial barrier function, fluid leakage, inflammation, and coagulopathy. A healthy endothelium may prevent these "secondary hit" complications, including possibly immunosuppression. Thus, the four pillars of whole body resynchronization during surgical trauma, and targets for new therapies, are: (1) the CNS, (2) the heart, (3) arterial supply and venous return functions, and (4) the endothelium. This is termed the Central-Cardio-Vascular-Endothelium (CCVE) coupling hypothesis. Since similar sterile injury cascades exist in critical illness, accidental trauma, hemorrhage, cardiac arrest, infection and burns, new drugs that improve CCVE coupling may find wide utility in civilian and military medicine.