gebhart/patrepeval huggingface dataset:
Protective versus pathologic pre-exposure cytokine profiles in dengue virus infection (2018)

These data demonstrate significant differences in pre-existing immune responses to DENV associated with the clinical outcome of subsequent infection. The finding of higher levels of some cytokines in subjects with symptomatic infection and higher levels of other cytokines in subjects with subclinical infection supports the existence of both protective and pathologic immune profiles. Clinical-immunological correlations identified in the context of natural DENV infection may be useful for evaluating immune responses to dengue vaccines.
Arthroscopy after anterior cruciate reconstruction with the Leeds-Keio ligament (1989)

The healing of anterior cruciate ligaments reconstructed with the Leeds-Keio artificial ligament was observed by arthroscopy in 42 knees and biopsy in 19 knees at intervals from 3 to 24 months after implantation. By three months the implant was covered with immature new tissue, and a dense vascular network crossed its surface. At 12 months a new ligament had developed and matured, looking like the natural one in most cases. Histology at this stage showed abundant collagenous fibres running parallel and longitudinally, while the synovial membrane showed no more than very slight inflammatory changes. By 18 to 24 months, the new ligament often had the arthroscopic appearance of a normal anterior cruciate ligament. These results suggest that this scaffold type of artificial ligament is effective for cruciate reconstruction, giving satisfactory healing without significant complications.
MIEDOS Y CREENCIA IRRACIONALES EN MUJERES VÍCTIMAS DE VIOLENCIA CONYUGAL (2004)

La presente investigacion tiene por objetivo describir los principales contenidos tanto de los miedos como de las creencias irracionales mas frecuentes, presentes en mujeres victimas de violencia conyugal, que a pesar del maltrato sufrido continuan conviviendo con el agresor. Para lo cual se trabajo en la Brigada de Proteccion a la Familia – Zona central de la ciudad de La Paz, Institucion donde se recibe el mayor numero de denuncias de violencia, tanto intrafamiliar como conyugal. Uno de los requisitos de seleccion para las participantes fue que sean casos reincidentes dentro de la Brigada, ya que esto da cuenta que las estrategias utilizadas para resolver el problema de violencia, en anteriores denuncias, no ha estado siendo efectiva, ya que estas mujeres vuelven a sentar nuevas denuncias. Para el estudio de la variable de miedos, se construyo un inventario, tomando en cuenta seis categorias de miedos centrales y para la variable de creencias, se realizo una adaptacion al registro de creencias propuesto por McKay y Davis, al cual se le hicieron cambios para que este sea adecuado al contexto al que se lo aplico. Despues de la aplicacion de ambos instrumentos a 40 mujeres que cumplian con los requisitos de investigacion, se procedio al analisis e interpretacion de los datos obtenidos. Dentro de este analisis se obtuvo que como contenidos centrales se encuentran el Miedo a las represalias fisicas, Miedo a perder a los hijos y Miedo a la indefension economica, de la misma forma dentro de las creencias las que se presentan de manera irracional son principalmente: “Se debe sentir miedo y ansiedad ante cualquier cosa desconocida, incierta o potencialmente peligrosa”, “Los acontecimientos externos son la mayoria de las desgracias de la humanidad” y “Es mas facil evitar los problemas de la vida que hacerles frente”.
CLXXIV.—Dyes derived from phenanthraquinone. Part III. Phenanthriminazoles (1923)

A. C. Sircar and G. C. Sircar, J. Chem. Soc., Trans., 1923, 123, 1559 DOI: 10.1039/CT9232301559
Replication-Competent Influenza A Virus That Encodes a Split-Green Fluorescent Protein-Tagged PB2 Polymerase Subunit Allows Live-Cell Imaging of the Virus Life Cycle (2011)

Studies on the intracellular trafficking of influenza virus ribonucleoproteins are currently limited by the lack of a method enabling their visualization during infection in single cells. This is largely due to the difficulty of encoding fluorescent fusion proteins within the viral genome. To circumvent this limitation, we used the split-green fluorescent protein (split-GFP) system (S. Cabantous, T. C. Terwilliger, and G. S. Waldo, Nat. Biotechnol. 23:102-107, 2005) to produce a quasi-wild-type recombinant A/WSN/33/influenza virus which allows expression of individually fluorescent PB2 polymerase subunits in infected cells. The viral PB2 proteins were fused to the 16 C-terminal amino acids of the GFP, whereas the large transcomplementing GFP fragment was supplied by transient or stable expression in cultured cells that were permissive to infection. This system was used to characterize the intranuclear dynamics of PB2 by fluorescence correlation spectroscopy and to visualize the trafficking of viral ribonucleoproteins (vRNPs) by dynamic light microscopy in live infected cells. Following nuclear export, vRNPs showed a transient pericentriolar accumulation and intermittent rapid (∼1 μm/s), directional movements in the cytoplasm, dependent on both microtubules and actin filaments. Our data establish the potential of split-GFP-based recombinant viruses for the tracking of viral proteins during a quasi-wild-type infection. This new virus, or adaptations of it, will be of use in elucidating many aspects of influenza virus host cell interactions as well as in screening for new antiviral compounds. Furthermore, the existence of cell lines stably expressing the complementing GFP fragment will facilitate applications to many other viral and nonviral systems.
Detection of HIV-1 Infection with a Green Fluorescent Protein Reporter System (1996)

Several systems for the detection of HIV-1 have been described in which HIV-1-susceptible cells contain a reporter gene (chloramphenicol acetyltransferase, beta-galactosidase, or alkaline phosphatase) under the control of the HIV-1 long terminal repeat (LTR). Upon infection by HIV-1, the expression of the viral tat product increases transcription from the HIV-1 LTR promoter, leading to high-level expression of the reporter gene product. Previously described reporter systems require processing of the cells by lysis, fixation, or other steps following infection to detect the reporter gene product. In the present study, the Aequorea green fluorescent protein S65T variant (GFP-S65T) was used in a reporter system for detecting HIV-1. HeLa-CD4 cells transfected with the plasmid pRH1, which encodes GFP-S65T under the control of the HIV-1 LTR promoter, and either co-transfected with a plasmid encoding the HIV-1 tat product or superinfected with HIV-1, expressed high levels of GFP-S65T, which was readily detected by fluorescence microscopy and fluorescence-activated cell-sorting analysis. The advantages of this system include its simplicity, sensitivity, and ability to detect and sort live HIV-1-infected cells using readily available instruments. The construction of cell lines stably transfected with pRH1 will provide a tool for titering HIV-1 and sorting HIV-1-infected cells.
Identification and cross‐directional control of coating processes (1992)

Abstract Of special industrial interest is the cross‐directional control of coating processes, where the cross direction refers to the direction perpendicular to the substrate movement. The objective of the controller is to maintain a uniform coating under unmeasured process disturbances. Assumptions that are relevant to coating processes found in industry are used to develop a model for control design. This model is used to derive a model predictive controller to maintain flat profiles of coating across the substrate by varying the liquid flows along the cross direction. Actuator constraints, measurement noise, model uncertainty, and the plant condition number are investigated to determine which of these limit the achievable closed‐loop performance. From knowledge of how these limitations affect the performance we can make some recommendations on how to modify the plant design to improve the coating uniformity. The theory developed throughout the article is rigorously verified through experiments on a pilot plant. The controller rejects disturbances with two sampling times. The proposed controller can reduce the variance in coating thickness by as much as 80% compared to what is possible by manual control or simple control schemes.
Biodistribution and dosimetric study in medullary thyroid cancer xenograft using bispecific antibody and iodine-125-labeled bivalent hapten. (1998)

This two-step targeting method seems very potent for the diagnosis and therapy of human medullary thyroid cancer and other CEA-producing tumors because it combines high tumor uptake and low normal tissue background.
Recognition of an expanded genetic alphabet by type-II restriction endonucleases and their application to analyze polymerase fidelity (2011)

To explore the possibility of using restriction enzymes in a synthetic biology based on artificially expanded genetic information systems (AEGIS), 24 type-II restriction endonucleases (REases) were challenged to digest DNA duplexes containing recognition sites where individual Cs and Gs were replaced by the AEGIS nucleotides Z and P [respectively, 6-amino-5-nitro-3-(1'-β-D-2'-deoxyribofuranosyl)-2(1H)-pyridone and 2-amino-8-(1'-β-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one]. These AEGIS nucleotides implement complementary hydrogen bond donor-donor-acceptor and acceptor-acceptor-donor patterns. Results allowed us to classify type-II REases into five groups based on their performance, and to infer some specifics of their interactions with functional groups in the major and minor grooves of the target DNA. For three enzymes among these 24 where crystal structures are available (BcnI, EcoO109I and NotI), these interactions were modeled. Further, we applied a type-II REase to quantitate the fidelity polymerases challenged to maintain in a DNA duplex C:G, T:A and Z:P pairs through repetitive PCR cycles. This work thus adds tools that are able to manipulate this expanded genetic alphabet in vitro, provides some structural insights into the working of restriction enzymes, and offers some preliminary data needed to take the next step in synthetic biology to use an artificial genetic system inside of living bacterial cells.
Dynamic Regulation of Tumor Growth and Metastasis by Heparan Sulfate Glycosaminoglycans (2002)

This article focuses on the emerging views and concepts concerning the role of cell surface and extracellular heparan sulfate-like glycosaminoglycans (HSGAGs) in tumor biology. HSGAGs, found ubiquitously both at the cell surface and in the extracellular matrix (ECM), play a critical role in regulating tumor initiation, progression, and metastasis. The diverse biological functions of HSGAGs include the regulation of coagulation, growth factor signaling, cell adhesion, proliferation, and mobility. HSGAGs, depending on their location (anchored at the cell surface or soluble as free GAGs), the signaling molecules they associate with, and their fine structures, can either promote or inhibit the tumorigenic process.
BACTERIAL METABOLISM OF 2-METHYLALANINE (1964)

Aaslestad, H. G. (Louisiana State University, Baton Rouge), and A. D. Larson. Bacterial metabolism of 2-methylalanine. J. Bacteriol. 88:1296-1303. 1964.-A bacterium isolated from soil was found to oxidize 2-methylalanine to 65% of the theoretical value by an adaptive enzyme system. Manometric experiments with whole cells indicated that acetone but not isopropylamine was an intermediate. Cellular extracts produced carbon dioxide from 2-methylalanine when pyruvate was added to the reaction mixture. Dialysis stimulated the 2-methylalanine metabolizing system, providing pyridoxal phosphate and pyruvate were supplied. A study of the stoichiometry of this pyridoxal phosphate-dependent reaction indicated that acetone and carbon dioxide were principal products. The formation of carbon dioxide from 2-methylalanine seemed dependent upon transfer of the amino group to pyridoxal phosphate. Other 2-methyl amino acids were found to be decarboxylated by this system, thus indicating a lack of absolute specificity.
Vascular and Neurogenic Rejuvenation of the Aging Mouse Brain by Young Systemic Factors (2014)

In the adult central nervous system, the vasculature of the neurogenic niche regulates neural stem cell behavior by providing circulating and secreted factors. Age-related decline of neurogenesis and cognitive function is associated with reduced blood flow and decreased numbers of neural stem cells. Therefore, restoring the functionality of the niche should counteract some of the negative effects of aging. We show that factors found in young blood induce vascular remodeling, culminating in increased neurogenesis and improved olfactory discrimination in aging mice. Further, we show that GDF11 alone can improve the cerebral vasculature and enhance neurogenesis. The identification of factors that slow the age-dependent deterioration of the neurogenic niche in mice may constitute the basis for new methods of treating age-related neurodegenerative and neurovascular diseases.
Immunization with a Lymphocytic Choriomeningitis Virus Peptide Mixed with Heat Shock Protein 70 Results in Protective Antiviral Immunity and Specific Cytotoxic T Lymphocytes (1998)

Heat shock proteins (hsp's) isolated from murine cancer cells can elicit protective immunity and specific cytotoxic T lymphocytes (CTLs) by channeling tumor-derived peptides bound to hsp's to the major histocompatibility class I antigen presentation pathway. Here we have investigated if hsp70 can be used in a novel peptide vaccine for the induction of protective antiviral immunity and memory CTLs. A CTL epitope from the well-defined lymphocytic choriomeningitis virus (LCMV) system was mixed with recombinant hsp70 in vitro under conditions that optimize peptide binding to hsp70. Mice were immunized with the hsp70-peptide mixture and challenged with LCMV. Virus titers were reduced 10-100-fold in these mice compared to control mice. Immunization with the hsp70-peptide mixture resulted in the development of CTL memory cells that could be reactivated during LCMV infection, and that in a 51Cr-release assay could lyse cells pulsed with the same peptide, but not cells pulsed with another LCMV peptide. These results show that hsp70 can be used with CTL epitopes to induce efficient protective antiviral immunity and the generation of peptide-specific CTLs. The results also demonstrate the usefulness of hsp70 as an alternative to adjuvants and DNA vectors for the delivery of CTL epitopes to antigen-presenting cells.
Real-time lip-synch face animation driven by human voice (2002)

In this demo, we present a technique for synthesizing the mouth movement from acoustic speech information. The algorithm maps the audio parameter set to the visual parameter set using the Gaussian mixture model and the hidden Markov model. With this technique, we can create smooth and realistic lip movements.
FDC-P1 myeloid cells engineered to express fibroblast growth factor receptor 1 proliferate and differentiate in the presence of fibroblast growth factor and heparin. (1992)

Full-length murine fibroblast growth factor (FGF) receptor 1 (FGFR-1L) cDNA was introduced into the FDC-P1 mouse myeloid progenitor cell line, which lacks FGF receptors and depends on interleukin 3 (IL-3) or granulocyte/macrophage colony-stimulating factor (GM-CSF) for its proliferation and survival. The expression of the FGFR-1L gene in FDC-P1 cells allowed these cells to grow in the presence of FGF and heparin. The resulting cell line, designated FD FGFR-1L.A, exhibited a more mature myeloid phenotype than did the parental FD FGFR-1L cells or uninfected FDC-P1 cells. They formed mainly dispersed colonies in soft-agar cultures when grown in the presence of FGF and heparin, suggestive of myeloid differentiation. The cells can be switched between growth on FGF/heparin and IL-3. Northern blot analysis and cytochemical staining demonstrated that FD FGFR-1L.A cells expressed myeloperoxidase mRNA and protein, biochemical markers specifically expressed during differentiation from the promyelocytic to the granulocytic stages, whereas the parental FD FGFR-1L cells and FDC-P1 cells failed to express this marker. These results indicate that the expression of FGFR-1L by FDC-P1 cells transmitted signals for growth in the presence of FGF and heparin and generated an additional signal for early myeloid differentiation but failed to commit FD FGFR-1L.A cells to terminal differentiation. This in vitro culture system can be used for molecular analysis of the regulation of cellular growth and differentiation mediated by the FGFs and their receptors.
Glycolipids of Metastatic Tissue in Liver from Colon Cancer: Appearance of Sialylated Lex and Lex Lipids1 (1988)

Glycolipids of metastatic tissue in liver from colon cancer were separated by HPLC and their structures were analyzed. Neutral glycolipid was composed of five major components. Four of them showed similar mobilities to GlcCer, LacCer, Gb3Cer, and nLc4Cer on TLC. The structure of the fifth major component was analyzed by exo-glycosidase treatments, 400 mHz proton NMR spectrometry, gas chromatography-mass spectrometry of the methylated sugars, and immunostaining on a TLC-plate, and concluded to be (Formula: see text). Ganglioside was composed of two major components. One of them was identical with GM3. The structure of the second ganglioside was analyzed by exo-glycosidase treatment, immunostaining on a TLC-plate, and gas chromatography-mass spectrometry of methylated sugars, and concluded to be (Formula: see text). These Le(x) and sialyl-Le(x) structure-containing lipids accounted for about 12% of the neutral glycolipid fraction and 42% of the ganglioside fraction, respectively. From these results, it appears that the biosynthetic pathway for Le(x) structure-containing lipids is activated in colon cancer.
Real-time TTCN for testing real-time and multimedia systems (1997)

In this paper we define real-time TTCN and apply it to several applications. In real-time TTCN, statements are annotated with time labels that specify their earliest and latest execution times. The syntactical extensions of TTCN are the definition of a table for the specification of time names and time units, and two new columns in the dynamic behaviour description tables for the annotation of statements with time labels. We define an operational semantics for real-time TTCN by mapping real-time TTCN to timed transition systems. Alternatively, we introduce a refined TTCN snapshot semantics that takes time annotations into account.
Cohort study of peanut and tree nut sensitisation by age of 4 years (1996)

IgE mediated allergy to peanuts is common in early childhood. In many the allergy persists but a minority may develop tolerance.
Interspecies transmission of the canine influenza H3N2 virus to domestic cats in South Korea, 2010 (2011)

In the past 4 years, incidences of endemic or epidemic respiratory diseases associated with canine influenza H3N2 virus in Asian dogs have been reported in countries such as South Korea and China. Canine species were considered to be the new natural hosts for this virus. However, at the beginning of 2010, influenza-like respiratory signs, such as dyspnoea, were also observed among cats as well as in dogs in an animal shelter located in Seoul, South Korea. The affected cats showed 100 % morbidity and 40 % mortality. We were able to isolate a virus from a lung specimen of a dead cat, which had suffered from the respiratory disease, in embryonated-chicken eggs. The eight viral genes isolated were almost identical to those of the canine influenza H3N2 virus, suggesting interspecies transmission of canine influenza H3N2 virus to the cat. Moreover, three domestic cats infected with intranasal canine/Korea/GCVP01/07 (H3N2) all showed elevated rectal temperatures, nasal virus shedding and severe pulmonary lesions, such as suppurative bronchopneumonia. Our study shows, for the first time, that cats are susceptible to canine influenza H3N2 infection, suggesting that cats may play an intermediate host role in transmitting the H3N2 virus among feline and canine species, which could lead to the endemic establishment of the virus in companion animals. Such a scenario raises a public health concern, as the possibility of the emergence of new recombinant feline or canine influenza viruses in companion animals with the potential to act as a zoonotic infection cannot be excluded.
Réactivité des N-vinylaziridines fonctionnalisées. Synthèse de dérivés des α,β-déhydro α-amino acides (1982)

Secondary 2-cyano- and 2-ethoxycarbonylaziridines react with acetylenic compounds and activated vinylic chlorides to give 2-cyano and 2-ethoxycarbonyl N-vinylaziridines. The nucleophile-catalysed isomerization of these compounds generally gives α,β-dehydro α-amino acid derivatives involving nucleophilic attack at C-3. Thermal isomerization, involving predominantly cleavage of the carbon–carbon bond of the ring, forms α-vinylamino esters and nitriles.
Immunological Models of Type 1 Diabetes (1999)

Type 1 diabetes mellitus is a serious health problem that affects several million new people each year. Although it is recognized that type 1 diabetes results from an autoimmune destruction of the insulin-producing beta-cells in the pancreatic islets of Langerhans, its etiopathogenesis is still not well understood. A certain genetic phenotype seems to be required, but it is not sufficient per se to trigger diabetes development. Numerous studies have pointed to the role of infectious agents as important environmental factors in breaking 'self'-tolerance and triggering activation of autoreactive T cells. Activated T cells, in turn, destroy target cells harboring the corresponding tissue-specific self-antigen, causing organ damage and loss of function. Several theories have been proposed to explain how environmental factors can initiate and/or perpetuate autoimmunity towards pancreatic beta-cells.
Ultrafast‐Pulse Measurement Methods (2008)

This chapter contains sections titled: Terminology and Definitions Electric Field Autocorrelation Measurements and the Power Spectrum Electric Field Cross-Correlation Measurements and Spectral Interferometry Intensity Correlation Measurements Chirped Pulses and Measurements in the Time–Frequency Domain Frequency-Resolved Optical Gating Pulse Measurements Based on Frequency Filtering Self-Referencing Interferometry Characterization of Noise and Jitter Problems
IMGT, the international ImMunoGeneTics information system, http://imgt.cines.fr. (2003)

IMGT, the international ImMunoGeneTics information system (http://imgt.cines.fr), is a high quality integrated knowledge resource specializing in immunoglobulins (IG), T cell receptors (TR) and major histocompatibility complexes (MHC) and related proteins of the immune system (RPI) of human and other vertebrates, created in 1989 by LIGM at the Université Montpellier II, CNRS, Montpellier, France. IMGT provides a common access to standardized data which include nucleotide and protein sequences, oligonucleotide primers, gene maps, genetic polymorphisms, specificities, and 2D and 3D structures. IMGT includes five databases (IMGT/LIGM-DB, IMGT/3Dstructure-DB, IMGT/MHC-DB, IMGT/PRIMER-DB, IMGT/GENE-DB) Web resources ('IMGT Marie-Paule page') and interactive tools (IMGT/V-QUEST, IMGT/JunctionAnalysis, IMGT/PhyloGene, IMGT/LocusView, IMGT/GeneView, IMGT/GeneSearch, IMGT/StructureQuery). IMGT data are expertly annotated according to the rules of the IMGT Scientific chart based on IMGTONTOLOGY. IMGT tools are particularly useful for the analysis of the IG and TR repertoires in physiological normal and pathological situations. IMGT has important applications in medical research (autoimmune diseases, AIDS, leukaemias, lymphomas, myelomas), biotechnology related to antibody engineering (phage displays, combinatorial libraries) and therapeutic approaches (graft, immunotherapy). IMGT is freely available at http://imgt.cines.fr.
Fundamentals of Physical Acoustics (2001)

Book review: Fundamentals of Physical Acoustics D.T. Blackstock; Wiley & Sons Ltd, New York, 2000, 541 pages, ISBN 0-471-3197