gebhart/patrepeval huggingface dataset:
Nucleotide chemistry. XVI. Phosporamidate analogs of oligonucleotides (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleotide chemistry. XVI. Phosporamidate analogs of oligonucleotidesRobert L. Letsinger and William S. MungallCite this: J. Org. Chem. 1970, 35, 11, 3800–3803Publication Date (Print):November 1, 1970Publication History Published online1 May 2002Published inissue 1 November 1970https://pubs.acs.org/doi/10.1021/jo00836a048https://doi.org/10.1021/jo00836a048research-articleACS PublicationsRequest reuse permissionsArticle Views227Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
ORC1 interacts with c‐Myc to inhibit E‐box‐dependent transcription by abrogating c‐Myc–SNF5/INI1 interaction (2000)

These results suggest that ORC1 suppresses the transcription activity of c-Myc by its recruitment into an inactive form of chromatin during some stage of the cell cycle.
The environment within: how gut microbiota may influence metabolism and body composition (2010)

Obesity, diabetes and consequently atherosclerotic vascular disease have become major health and public health issues worldwide. The increasing and staggering prevalence of obesity might not only be explained by nutritional habits or the reduction of energy expenditure through decreased physical activity. In addition, recent studies have focused on intestinal microbiota as environmental factors that increase energy yield from diet, regulate peripheral metabolism and thereby increase body weight. Obesity is associated with substantial changes in composition and metabolic function of gut microbiota, but the pathophysiological processes driving this bidirectional relationship have not been fully elucidated. This review discusses the relationships between the following: composition of gut microbiota, energy extracted from diet, synthesis of gut hormones involved in energy homeostasis, production of butyrate and the regulation of fat storage.
Anti-HER2 Treatment and Breast Cancer: State of the Art, Recent Patents, and New Strategies (2008)

HER2 is a transmembrane tyrosine kinase receptor in the EGFR (epidermal growth factor receptor) family. The role of HER2 has been most thoroughly studied in breast cancer, in which constitutively active HER2 is overexpressed in 18-22% of cases and is correlated with a poor prognosis. Hence, effective inhibition of the constitutive HER2 signaling in cancer cells has been a major goal in the design of therapies. Therapeutic targeting of HER2 with humanized antibodies such as trastuzumab (Herceptin TM, Genentech) South San Francisco, CA) has proven to be an effective approach for the treatment of breast cancer cells that over-express HER2. The encouraging results of trastuzumab in patients with metastatic and early breast cancer diseases have prompted the evaluation of new HER2 inhibitors for increasing the potential for combinatorial therapies. This review will focus on patents that target HER2 in anti-cancer treatment.
Multilayer Silicon Nitride-on-Silicon Integrated Photonic Platforms and Devices (2015)

We review and present additional results from our work on multilayer silicon nitride (SiN) on silicon-on-insulator (SOI) integrated photonic platforms over the telecommunication wavelength bands near 1550 and 1310 nm. SiN-on-SOI platforms open the possibility for passive optical functionalities implemented in the SiN layer to be combined with active functionalities in the SOI. SiN layers can be integrated onto SOI using a front-end or back-end of line integration process flow. These photonic platforms support low-loss SiN waveguides, low-loss and low-crosstalk waveguide crossings, and low-loss interlayer transitions using adiabatic tapers. Novel ultra-broadband and efficient grating couplers as well as polarization management devices are enabled by the close coupling between the silicon and SiN layers.
Observations of amplitude scintillations on a low-elevation Earth-space path (1984)

Results of amplitude-scintillation measurements at 11 GHz on an 8.9° elevation path from an INTELSAT V satellite are presented and briefly compared with those obtained from OTS at 30° of elevation. The variability of the standard deviation of the process is illustrated and discussed in the context of the long-term distribution. Spectral density analysis reveals the familiar bandlimited noise type of spectrum with a 3 dB corner frequency of about 60 mHz.
von Willebrand's disease in Dobermann dogs in Australia (1995)

Over a 5-year period (1988-92), von Willebrand factor antigen (vWf:Ag) concentrations were determined on plasma samples from 614 Dobermanns. The vWf:Ag concentration was < 50 canine units (CU)/dL in 373 dogs (61%); these dogs were classified as carriers of the von Willebrand's disease (vWD) gene. In order to identify which dogs were at risk of haemorrhage due to vWD, we determined a cut-off vWf:Ag concentration below which dogs could be considered at risk. This cut-off was chosen in order to minimise the number of dogs genuinely at risk of haemorrhage, being wrongly classified as not at risk. This was done without sacrificing the specificity of the cut-off to any great extent. A vWf:Ag concentration of < 36 CU/dL was empirically chosen as the optimum cut-off concentration. In 282 dogs (76% of the carriers), the vWf:Ag concentration was below this cut-off and these dogs were, thus, classified as being at risk of haemorrhage due to vWD. Haemorrhage attributable to vWD was seen in 107 dogs (29% of the carriers, or 17% of all the dogs). Haemorrhage mostly followed trauma or surgery, but spontaneous genito-urinary and gastrointestinal haemorrhages were also frequent. Of these dogs, 92 were of known age, with a median of 3 years, and 102 were of known sex, with 61% being female. In 89 dogs in which the severity of haemorrhage was subjectively assessed, mild and moderate bleeding occurred with similar frequency (48% and 43%, respectively). There were 8 cases of severe haemorrhage, with two deaths.(ABSTRACT TRUNCATED AT 250 WORDS)
Interleukin-1 TNFα and Their Naturally Occurring Antagonists in Sepsis (1993)

Recent evidence has demonstrated that specific inhibitors of the proinflammatory cytokines, IL-1 and TNF alpha, circulate in the blood of healthy individuals and concentrations of these inhibitors are elevated in inflammation. Initial characterization reveals that one of the classes of IL-1 inhibitors that circulates is the newly described IL-1 receptor antagonist (IL-1ra). A class of newly described TNF inhibitors is the soluble type I (p55) and type II (p75) TNF receptor (sTNFR). In patients with nonlethal infections, both IL-1ra and sTNFR appear to circulate at levels that can block the quantities of IL-1 and TNF alpha produced. However, such concentrations of inhibitors are inadequate to prevent the deleterious host responses to exaggerated proinflammatory concentrations seen in lethal septic shock. Nevertheless, administering either IL-1ra or soluble TNF receptors to raise plasma concentrations 1,000-fold can prevent the pathologic sequelae associated with excessive cytokine production.
Identification of new DNA markers close to the myotonic dystrophy locus. (1991)

The most useful markers for the prenatal diagnosis of myotonic dystrophy (DM) are APOC2 and CKM, both of which map proximal to DM. In order to produce other markers useful for DM, we have screened genomic DNA libraries constructed from cell line 20XP3542-1-4, which contains 20 to 30 Mb of human material including APOC2 and CKM. Of 51 human clones identified, seven map to chromosome 17, four to chromosome 8, and nine to chromosome 19, and the remaining 31 were excluded form chromosome 19 but not localised further. Four of the clones from chromosome 19 map distal to CKM and two of these clones (D19S62 and D19S63) are closely linked to DM. Analysis of a family in which a crossover between CKM and DM has occurred shows that neither D19S62 nor D19S63 and DM have recombined, suggesting that D19S62 and D19S63 are either closer to or flanking DM in relation to CKM. Pulsed field gel analysis showed that CKM, D19S62, and D19S63 map to a region of at least 1500 kb.
Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference (2002)

RNA interference (RNAi) is an ancient antiviral response that processes dsRNA and associates it into a nuclease complex that identifies RNA with sequence homology and specifically cleaves it. We demonstrate that RNAi mediated by 21-bp dsRNA specifically inhibits HIV-1 infection of permanent cell lines and primary CD4(+) T cells. Inhibition of HIV replication was measured by p24 Gag protein content in supernatant, Northern blot analysis, and DNA PCR for products of reverse transcription. The inhibition occurred at two points in the viral life cycle, after fusion and before reverse transcription and during transcription of viral RNA from integrated provirus. Treatment of HIV-infected activated CD4(+) T cells with a fluorine-derivatized siRNA that is resistant to RNase A yielded similar inhibition of HIV infection. In addition, the derivatized siRNA could be delivered without lipofectin complexing and in the presence of serum. The identification of RNAi activity against HIV-1 presents a new approach to study viral infections and a proof of concept of RNAi antiviral activity in mammalian cells.
Escherichia coli alkaline phosphatase fails to acquire disulfide bonds when retained in the cytoplasm (1991)

The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synthesis of the protein was completed. This contrasted with the normally exported protein, wherein disulfide bonds formed rapidly. Disulfide bond formation accompanied export and processing. The implications of these findings for the inactivity of the enzyme in the cytoplasm are discussed.
Synthesis and DNA Cleavage Study of a 10-Membered Ring Enediyne Formed via Allylic Rearrangement (1999)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSynthesis and DNA Cleavage Study of a 10-Membered Ring Enediyne Formed via Allylic RearrangementWei-Min Dai, Kin Chiu Fong, Chi Wai Lau, Ling Zhou, Wataru Hamaguchi, and Sei-ichi NishimotoView Author Information Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China, and Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan Cite this: J. Org. Chem. 1999, 64, 3, 682–683Publication Date (Web):January 13, 1999Publication History Received8 October 1998Published online13 January 1999Published inissue 1 February 1999https://pubs.acs.org/doi/10.1021/jo9820263https://doi.org/10.1021/jo9820263rapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views270Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Alcohols,Crystal cleavage,Genetics,Organic compounds,Rearrangement Get e-Alerts
Optical feedback phenomena in semiconductor lasers (2002)

Optical feedback phenomena in semiconductor lasers attracted considerable interest during the last decade. It has been recognized quite early, that the linewidth can be reduced considerably with optical feedback and external cavity lasers are routinely used if linewidths down to a few kHz are required. However, optical feedback phenomena have been found to be responsible also for mode hopping, for strong excess noise in the coherence collapse regime and other regimes have been identified with stable microwave oscillations.
An Integrating Vector for Tunable, High Copy, Stable Integration into the Dispersed Ty δ Sites of Saccharomyces cerevisiae (1996)

We have constructed a yeast integration vector targeted to chromosomal Ty delta sequences and used it to create Saccharomyces cerevisiae strains with stable tandem integrations ranging from 1 to 30 vector copies. The vector carries the bacterial NEO gene, allowing copy number to be tuned by varying G418 resistance, which generally increases with copy number as determined by quantitative Southern blot. Tandem integration into a single site is most commonly observed, but single-copy and two-site integration is also observed. Bovine pancreatic trypsin inhibitor was constitutively expressed and secreted using the NEO-based delta vector, and secretion levels were 2-10-fold improved relative to commonly used 2 mu multicopy yeast plasmids. The NEO-based Ty delta vector is a powerful tool for stable heterologous protein expression and secretion in yeast.
A Novel Method for Authentication Optimization during Handover in Heterogeneous Wireless Networks (2007)

This paper presents a solution for optimizing network access authentication procedure and security context transfer during vertical handover in heterogeneous wireless networks (HWN). Security in HWN is a major challenge due to the vastly different security architectures used within each network. Currently, standardization working groups study the optimization of authentication procedure and security context transfer for seamless handover between heterogeneous networks. The goal of this paper is to provide a security frame work that accounts for heterogeneity in wireless access networks without degrading the level of security currently provided by the individual wireless systems. We propose a new frame work for access network specific authentication optimization and security context transfer during vertical handover preparation phase. We also demonstrate how the proposed security frame work provides improvement in the handover performance.
Cleavage of the Amino-Terminal Domain of Cd36 by Hiv-1 Protease (1995)

Abstract: The HIV-I protease has been found to cleave the integral membrane protein CD36 at two specific sites (residues 43*44 and 67*68) in the amino-terminal region of the bovine protein. Neither cleavage site conforms to previously identified type I or type 2 sites characteristic of HIV-1. Human CD36, which differs only slightly in its amino acid sequence.Was not cleaved by the HIV-1 protease.
Molecular Basis of Fructose Utilization by the Wine YeastSaccharomyces cerevisiae: a MutatedHXT3Allele Enhances Fructose Fermentation (2007)

Fructose utilization by wine yeasts is critically important for the maintenance of a high fermentation rate at the end of alcoholic fermentation. A Saccharomyces cerevisiae wine yeast able to ferment grape must sugars to dryness was found to have a high fructose utilization capacity. We investigated the molecular basis of this enhanced fructose utilization capacity by studying the properties of several hexose transporter (HXT) genes. We found that this wine yeast harbored a mutated HXT3 allele. A functional analysis of this mutated allele was performed by examining expression in an hxt1-7Delta strain. Expression of the mutated allele alone was found to be sufficient for producing an increase in fructose utilization during fermentation similar to that observed in the commercial wine yeast. This work provides the first demonstration that the pattern of fructose utilization during wine fermentation can be altered by expression of a mutated hexose transporter in a wine yeast. We also found that the glycolytic flux could be increased by overexpression of the mutant transporter gene, with no effect on fructose utilization. Our data demonstrate that the Hxt3 hexose transporter plays a key role in determining the glucose/fructose utilization ratio during fermentation.
A transfected human fucosyltransferase cDNA determines biosynthesis of oligosaccharide ligand(s) for endothelial-leukocyte adhesion molecule I (1991)

Conference Article| August 01 1991 A transfected human fucosyltransferase cDNA determines biosynthesis of oligosaccharide ligand(s) for endothelial-leukocyte adhesion molecule I John B. Lowe; John B. Lowe †Howard Hughes Medical Institute, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A.*Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Lloyd M. Stoolman; Lloyd M. Stoolman *Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Rajan P. Nair; Rajan P. Nair †Howard Hughes Medical Institute, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Robert D. Larsen; Robert D. Larsen †Howard Hughes Medical Institute, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Terry L. Behrend; Terry L. Behrend *Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Rory M. Marks Rory M. Marks †Department of Internal Medicine, The University of Michigan Medical School, Ann Arbor, Michigan 48109–0650 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1991) 19 (3): 649–653. https://doi.org/10.1042/bst0190649 Article history Received: April 10 1991 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation John B. Lowe, Lloyd M. Stoolman, Rajan P. Nair, Robert D. Larsen, Terry L. Behrend, Rory M. Marks; A transfected human fucosyltransferase cDNA determines biosynthesis of oligosaccharide ligand(s) for endothelial-leukocyte adhesion molecule I. Biochem Soc Trans 1 August 1991; 19 (3): 649–653. doi: https://doi.org/10.1042/bst0190649 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: ELAM-1, endothelial-leukocyte adhesion molecule 1, CRD, carbohydrate recognition domains, HUVEC, human umbilical vein endothelial cell, sLex, sialyl Lewis x tetrasaccharide, Lex, neutral Lewis x trisaccharide, Lea, Lewis a oligosaccharide, sLea, sialyl Lewis a oligosaccharide, TNFα, tumour necrosis factor α, CHO, Chinese hamster ovary This content is only available as a PDF. © 1991 Biochemical Society1991 Article PDF first page preview Close Modal You do not currently have access to this content.
Polarization Coupling of Light and Optoelectronics Devices Based on Periodically Poled Lithium Niobate (2010)

Polarization Coupling of Light and Optoelectronics Devices Based on Periodically Poled Lithium Niobate
A 1,536-Well [ 35 S]GTP γ S Scintillation Proximity Binding Assay for Ultra-High-Throughput Screening of an Orphan G α i-Coupled GPCR (2008)

Members of the superfamily of seven transmembrane receptors, known as G protein-coupled receptors (GPCRs), are important targets for many therapeutic areas in drug discovery. A homogeneous guanosine 5'-O-(3-[(35)S]thio)triphosphate ([(35)S]GTPgammaS) scintillation proximity assay (SPA) binding assay targeting a Galphai-coupled GPCR recombinantly expressed in membranes of Chinese hamster ovary (CHO) cells was developed and miniaturized into 1,536-well plate format. The primary ultra-high-throughput screen of the entire compound collection was accomplished on the Kalypsys (San Diego, CA) robotic platform at a concentration of 8 muM using the 1,536-well [(35)S]GTPgammaS SPA binding functional assay. The signal-to-noise ratio of the primary screen was approximately 2.1-fold, and the plate coefficient of variation for the compound field was approximately 11%. The hit rate from the primary screen for receptor agonists at >35% activity was approximately 0.3%. Primary hits were cherry-picked, confirmed in triplicate, counterscreened against untransfected CHO cell membranes, and further analyzed in a cyclic AMP functional assay, resulting in 34 leads for optimization.
[Long-term experimental electric stimulation of denervated laryngeal muscles in dogs. Myopathologic findings]. (1987)

The recurrent laryngeal nerve was transsected unilaterally and a device for electrical stimulation of the denervated muscles was implanted in 6 dogs. After 3 to 11 months the laryngeal muscles were obtained and histopathologically demonstrated a striking neurogenic pattern. In place of homogeneous muscle fiber atrophy, a patchwork of grouped fibre atrophy, compensational hypertrophy, fibre regeneration and reinnervation as well as muscle fibre type grouping were observed. Muscle fibre type distribution in the m. cricoarytenoideus posterior, m. vocalis and m. cricothyroideus was determined and compared between the normal and denervated sites. All muscles showed some changes in the fibre type distribution, including type grouping, but these were most pronounced in m. vocalis. The average fibre diameter decreased whereas fibre size variability increased. Mixed muscle fibre atrophy with compensatory hypertrophy of a single fibre type predominated. There was no evidence of disuse atrophy. The electrically stimulated muscles could be maintained at a reduced functional level throughout the study period.