gebhart/patrepeval huggingface dataset:
Human Monoclonal Antibodies to Human Cytomegalovirus (1989)

Human monoclonal antibodies (HMAbs) to human cytomegalovirus (HCMV) have been developed by using electric field-induced cell fusion of human B lymphocytes to the human-mouse cell line SBC-H20. By this procedure, multiple hybridomas have been produced that secrete IgG 1 HMAbs with distinct patterns of indirect immunofluorescence on HCMV-infected cells. HMAbs Z01 and X20 immunoprecipitated a major protein at 64 kDa. HMAb Z02 immunoprecipitated a major protein of 48-50 kDa. HMAb Z10 identified a single protein at 65 kDa and HMAb X16 identified proteins at 100, 65, and 36-38 kDa. The HMAbs demonstrated varying degrees of virus-neutralizing activity. The production of HMAbs to HCMV provides an important approach to studying the human host response to HCMV by elucidating biologically relevant antigens and epitopes. In addition, HMAbs are a potentially unlimited source of relevant human antibodies for treating life-threatening HCMV infection.
Characterization of Farmington virus, a novel virus from birds that is distantly related to members of the family Rhabdoviridae (2013)

In sum, our analysis indicates that FARV represents a new species within the family Rhabdoviridae.
Diarrhea and Campylobacter Infections in Patients Infected with the Human Immunodeficiency Virus (1989)

Journal Article Diarrhea and Campylobacter Infections in Patients Infected with the Human Immunodeficiency Virus Get access E. Bernard, E. Bernard Infectious and Tropical Diseases, Laboratory of Bacteriology, and Internal Medicine and Medical Reanimation, Archet Hospital, Nice, France Please address requests for reprints to Dr. E. Bernard, Infectious Diseases, Archet Hospital BP 689 06012, Nice cedex, France. Search for other works by this author on: Oxford Academic PubMed Google Scholar P. M. Roger, P. M. Roger Infectious and Tropical Diseases, Laboratory of Bacteriology, and Internal Medicine and Medical Reanimation, Archet Hospital, Nice, France Search for other works by this author on: Oxford Academic PubMed Google Scholar D. Carles, D. Carles Infectious and Tropical Diseases, Laboratory of Bacteriology, and Internal Medicine and Medical Reanimation, Archet Hospital, Nice, France Search for other works by this author on: Oxford Academic PubMed Google Scholar V. Bonaldi, V. Bonaldi Infectious and Tropical Diseases, Laboratory of Bacteriology, and Internal Medicine and Medical Reanimation, Archet Hospital, Nice, France Search for other works by this author on: Oxford Academic PubMed Google Scholar J. P. Fournier, J. P. Fournier Infectious and Tropical Diseases, Laboratory of Bacteriology, and Internal Medicine and Medical Reanimation, Archet Hospital, Nice, France Search for other works by this author on: Oxford Academic PubMed Google Scholar P. Dellamonica P. Dellamonica Infectious and Tropical Diseases, Laboratory of Bacteriology, and Internal Medicine and Medical Reanimation, Archet Hospital, Nice, France Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 159, Issue 1, January 1989, Pages 143–144, https://doi.org/10.1093/infdis/159.1.143 Published: 01 January 1989
Alison Earns Reward for her Passion and Drive (2008)

Alison Waters of St. Michael's Collegiate Senior Library has been awarded the inaugural Doreen Hopkins Medal by ASLA (Tasmania). She has been given the award in recognition of her drive and professional vision.
Polymers carrying sLe x ‐mimetics are superior inhibitors of E‐selectin‐dependent leukocyte rolling in vivo (2003)

Selectins mediate leukocyte rolling and may represent good anti-inflammatory drug targets. Detailed knowledge regarding the structure of selectin ligands has permitted development of selectin antagonists with varying specificities and activity. Efficacy of monovalent selectin antagonists may be increased by presenting them on a polymer backbone. We have synthesized a range of multivalent selectin antagonists and characterized their activity by using intravital microscopy of the mouse cremaster muscle. The monovalent inhibitor CGP77175A inhibited E-selectin-dependent leukocyte rolling at a dose of 3 mg/kg. Multivalent presentation of CGP77175A on a modified polylysine backbone (degree of polymerization = 1200; 50% of the polylysines carry the inhibitor) greatly enhanced in vivo activity giving an inhibitor that produced an equivalent effect at 0.1 mg/kg. The polylysine conjugate was also longer acting than the monovalent antagonist. In spite of greatly enhanced activity against E-selectin compared with monovalent inhibitor, the multivalent inhibitor had no measurable effect on P- or L-selectin-dependent leukocyte rolling.
EORTC 18961: Post-operative adjuvant ganglioside GM2-KLH21 vaccination treatment vs observation in stage II (T3-T4N0M0) melanoma: 2nd interim analysis led to an early disclosure of the results (2008)

9004 Background: EORTC 18961 assessed in the largest adjuvant phase III trial to date in stage II melanoma the efficacy and toxicity of Ganglioside GM2-KLH21 Vaccination (VAC) Treatment vs Observation (OBS). Methods: Patients (Pts) were observed or received vaccine sc once weekly week 1–4, every 3 months from week 12 for first 2 years and every 6 months during third year (total of 14 vaccinations). Stratification factors for randomization were: Breslow thickness, ulceration, being staged yes vs no by Sentinel Node (SNLD) or Elective Lymph Node Dissection (ELND), sex and institution. Relapse-free survival (RFS) was the primary endpoint; Distant metastasis-free survival (DMFS) and overall survival (OS) were the prespecified secondary endpoints. Intent-to-treat analysis was performed. Results: Between March 2002 and Dec. 2005, 1,314 pts entered the trial. Patient demographics showed perfect balance for prognostic factors. The 2nd interim analysis was performed when 267 RFS events were reported, i.e. 67% of the total number required at final analysis. Median follow-up was 1.8 years. Similar treatment differences were obtained in lympnhnode-staged (N=644) or non-staged (N=670) patients. Grade 3 - toxicities occurred in < 2%.(VAC) and in < 1% (OBS). Grade 2 - toxicities comprised fatigue (15% vs 3%), fever (9% vs 1%), nausea (3% vs 1%). Local grade 3 - toxicity occurred in 4 % (VAC). The EORTC IDMC reviewed both safety and efficacy data. For the primary endpoint, RFS, the criteria for stopping for futility were met. For DMFS and OS, the results pointed in the direction of a detrimental effect of the vaccine. The IDMC recommended trial 18961 to be stopped and vaccinations to be halted in pts still receiving VAC. Pts should continue to be followed for the trial’s endpoints. Conclusions: Adjuvant GM2-KLH21 vaccination is ineffective and could even be detrimental in stage II melanoma patients. RFS DMFS OS Obs. GM2-KLH21 Obs. GM2-KLH21 Obs. GM2-KLH21 Nb. Events 132 135 65 86 35 56 2-yr % (SE%) 77.8% (1.9%) 75.1% (2.0%) 88.5 (1.6%) 82.4 (1.9%) 92.4 (1.4%) 89.2 (1.7%) HR (CI)(1) 1.02 (0.77-1.36)* 1.33 (0.77-2.28)** 1.57 (0.68-3.64)*** p- value(1) 0.85 0.08 0.03 HR (CI)(2) 1.00 (0.75-1.34)* 1.32 (0.76- 2.30)** 1.66 (0.66-3.99)*** p-value(2) 0.99 0.10 0.02 HR = Hazard Ratio; * 98% CI, ** 99.9% CI, *** 99.99% CI. (1) Cox model, unadjusted for stratification factors; (2) Cox model, stratified for stratification factors No significant financial relationships to disclose.
Dronabinol increases pain threshold in patients with functional chest pain: a pilot double-blind placebo-controlled trial (2016)

Noncardiac chest pain is associated with poor quality of life and high care expenditure. The majority of noncardiac chest pain is either gastresophageal reflux disease related or due to esophageal motility disorders, and the rest are considered functional chest pain (FCP) due to central and peripheral hypersensitivity. Current treatment of FCP improves 40-50% of patients. Cannabinoid receptors 1 (CB1) and 2 (CB2) modulate release of neurotransmitters; CB1 is located in the esophageal epithelium and reduces excitatory enteric transmission and potentially could reduce esophageal hypersensitivity. We performed a prospective study to evaluate its effects on pain threshold, frequency, and intensity in FCP. Subjects with FCP received dronabinol (5 mg, twice daily; n = 7; average age, 44 years; mean body mass index, 26.7) or placebo (n = 6; average age, 42 years; mean body mass index, 25.9) for 28 days (4 weeks). Chest pain, general health, and anxiety/depression questionnaires were assessed at baseline and at 4 weeks. Subjects underwent an esophageal balloon distention test prior to treatment and on last day of the study. Dronabinol increased pain thresholds significantly (3.0 vs. 1.0; P = 0.03) and reduced pain intensity and odynophagia compared to placebo (0.18 vs. 0.01 and 0.12 vs. 0.01, respectively, P = 0.04). Depression and anxiety scores did not differ between the groups at baseline or after treatment. No significant adverse effects were observed. In this novel study, dronabinol increased pain threshold and reduced frequency and intensity of pain in FCP. Further, large scale studies are needed to substantiate these findings.
Tissue-Specific Methylation of Human Insulin Gene and PCR Assay for Monitoring Beta Cell Death (2014)

The onset of metabolic dysregulation in type 1 diabetes (T1D) occurs after autoimmune destruction of the majority of pancreatic insulin-producing beta cells. We previously demonstrated that the DNA encoding the insulin gene is uniquely unmethylated in these cells and then developed a methylation-specific PCR (MSP) assay to identify circulating beta cell DNA in streptozotocin-treated mice prior to the rise in blood glucose. The current study extends to autoimmune non-obese diabetic (NOD) mice and humans, showing in NOD mice that beta cell death occurs six weeks before the rise in blood sugar and coincides with the onset of islet infiltration by immune cells, demonstrating the utility of MSP for monitoring T1D. We previously reported unique patterns of methylation of the human insulin gene, and now extend this to other human tissues. The methylation patterns of the human insulin promoter, intron 1, exon 2, and intron 2 were determined in several normal human tissues. Similar to our previous report, the human insulin promoter was unmethylated in beta cells, but methylated in all other tissues tested. In contrast, intron 1, exon 2 and intron 2 did not exhibit any tissue-specific DNA methylation pattern. Subsequently, a human MSP assay was developed based on the methylation pattern of the insulin promoter and human islet DNA was successfully detected in circulation of T1D patients after islet transplantation therapy. Signal levels of normal controls and pre-transplant samples were shown to be similar, but increased dramatically after islet transplantation. In plasma the signal declines with time but in whole blood remains elevated for at least two weeks, indicating that association of beta cell DNA with blood cells prolongs the signal. This assay provides an effective method to monitor beta cell destruction in early T1D and in islet transplantation therapy.
The Protective Effect of L-Arginine on Ischemia-Reperfusion Injury in Rat Skin Flaps (1997)

The objective of this study was to examine whether the administration of L-arginine, a precursor of nitric oxide and substrate of nitric oxide synthase, prior to reperfusion could lead to decrease in neutrophil-mediated tissue injury and improved flap survival. Epigastric island skin flaps were elevated in 70 rats and rendered ischemic. Thirty minutes prior to reperfusion, the rats were treated with intraperitoneal saline (n = 15), L-arginine (n = 15), D-arginine (n = 15), or N omega-nitro-L-arginine methylester plus L-arginine in equimolar amounts (n = 15). Flap survival at 7 days and neutrophil counts at 24 hours were evaluated. Flap necrosis as expected in the sham group of animals (n = 10) was 0.0 percent, while the control (saline-treated) animals had 59.6 percent necrosis. Animals treated with L-arginine demonstrated a significant decrease in flap necrosis to 12.7 percent. This protective effect was almost completely negated by N omega-nitrol-L-arginine methylester, which significantly increased flap necrosis to 49.3 percent and was much less pronounced with D-arginine (28.6 percent). Neutrophil counts were significantly decreased in flaps from L-arginine-treated and sham animals versus both saline and N omega-nitro-L-arginine methylester-treated groups. We conclude that administration of L-arginine prior to reperfusion can significantly reduce the extent of flap necrosis and flap neutrophil counts due to ischemia-reperfusion injury. This protective effect is completely negated by nitric oxide synthase inhibition. Since L-arginine reduces the number of neutrophils within the flap and the extent of flap necrosis only in the presence of active nitric oxide synthase, we hypothesize that this protective effect of L-arginine on ischemia-reperfusion injury is secondary to a nitric oxide-mediated suppression of neutrophil-mediated injury.
Aspects of Plant Intelligence (2003)

Intelligence is not a term commonly used when plants are discussed. However, I believe that this is an omission based not on a true assessment of the ability of plants to compute complex aspects of their environment, but solely a reflection of a sessile lifestyle. This article, which is admittedly controversial, attempts to raise many issues that surround this area. To commence use of the term intelligence with regard to plant behaviour will lead to a better understanding of the complexity of plant signal transduction and the discrimination and sensitivity with which plants construct images of their environment, and raises critical questions concerning how plants compute responses at the whole-plant level. Approaches to investigating learning and memory in plants will also be considered.
Wnt11 and Wnt7a are up‐regulated in association with differentiation of cardiac conduction cells in vitro and in vivo (2003)

The heart beat is coordinated by a precisely timed sequence of action potentials propagated through cells of the conduction system. Previously, we have shown that conduction cells in the chick embryo are derived from multipotent, cardiomyogenic progenitors present in the looped, tubular heart. Moreover, analyses of heterogeneity within myocyte clones and cell birth dating have indicated that elaboration of the conduction system occurs by ongoing, localized recruitment from within this multipotent pool. In this study, we have focused on a potential role for Wnt signaling in development of the cardiac conduction system. Treatment of embryonic myocytes from chick with endothelin-1 (ET-1) has been shown to promote expression of markers of Purkinje fiber cells. By using this in vitro model, we find that Wnt11 are Wnt7a are up-regulated in association with ET-1 treatment. Moreover, in situ hybridization reveals expression, although not temporal coincidence of, Wnt11 and Wnt7a in specialized tissues in the developing heart in vivo. Specifically, whereas Wnt11 shows transient and prominent expression in central elements of the developing conduction system (e.g., the His bundle), relative increases in Wnt7a expression emerge at sites consistent with the location of peripheral conduction cells (e.g., subendocardial Purkinje fibers). The patterns of Wnt11 and Wnt7a expression observed in vitro and in the embryonic chick heart appear to be consistent with roles for these two Wnts in differentiation of cardiac conduction tissues.
Down‐regulation of pescadillo inhibits proliferation and tumorigenicity of breast cancer cells (2009)

Pescadillo, which has been found to be involved in the process of ribosomal biogenesis, has been demonstrated to play a role in embryonic development, DNA replication, and gene transcription. While deregulation of ribosomal biogenesis was also found to contribute to carcinogenesis, and proteins that regulate ribosomal biogenesis are commonly overexpressed in primary tumors, little is known about the clinical significance and biological function of pescadillo in human breast cancer. In the current study, we found that the expression of pescadillo was markedly up-regulated in human breast cancer cells and tissues at both mRNA and protein levels. Immunohistochemical analysis revealed that pescadillo expression in clinical stage I-IV primary breast cancer tissues was statistically significantly higher than that in normal breast tissues (P < 0.05). Furthermore, we demonstrated that knockdown pescadillo with RNAis inhibited cell proliferation and the colony-forming ability of the cells. Anchorage-independent growth ability assay indicated that ablation of pescadillo led to the reduction of breast cancer cells tumorigenicity in vitro. Moreover, depletion of endogenous pescadillo resulted in decreased expression of cell cycle protein cyclin D1 and up-regulation of cyclin-dependent kinase inhibitor p27(Kip1), as well as attenuated protein kinase B (Akt)/glycogen synthase kinase 3 beta (GSK-3beta) signaling. Taken together, our results suggest that pescadillo might play a role in promoting the proliferation and carcinogenesis of human breast cancer, and thereby might be a potential target for human breast cancer treatment.
Two Soluble forms of Glutamate Dehydrogenase Isoproteins from Bovine Brain (1995)

Two soluble forms of novel glutamate dehydrogenase isoproteins, designated GDH I and GDH II, have been purified from bovine brain. GDH I and GDH II were separated on a hydroxyapatite column and eluted by a step gradient at different phosphate concentrations (30 mM and 50 mM for GDH I and GDH II, respectively). The preparations were homogeneous on SDS/PAGE. GDH I and GDH II showed similarity in their molecular sizes and are composed of six identical subunits having a molecular size of 57,500 Da. Differences between the biochemical properties of GDH I and GDH II, such as N-terminal amino acid sequences of intact and tryptic-digested enzymes, kinetic parameters, optimum pH and heat stability, were extensively examined in both reductive amination of alpha-oxoglutarate and oxidative deamination of glutamate. The different effects of ADP on GDH isoproteins were also studied under various conditions. These results indicate that GDH I and GDH II, isolated from bovine brain, are novel and distinct polypeptides.
Tracking by Natural Language Specification (2017)

This paper strives to track a target object in a video. Rather than specifying the target in the first frame of a video by a bounding box, we propose to track the object based on a natural language specification of the target, which provides a more natural human-machine interaction as well as a means to improve tracking results. We define three variants of tracking by language specification: one relying on lingual target specification only, one relying on visual target specification based on language, and one leveraging their joint capacity. To show the potential of tracking by natural language specification we extend two popular tracking datasets with lingual descriptions and report experiments. Finally, we also sketch new tracking scenarios in surveillance and other live video streams that become feasible with a lingual specification of the target.
Et3B Induced Radical Addition of Thiols to Acetylenes (1987)

Abstract Thiols added easily to acetylenic compounds in the presence of Et3B to give alkenyl sulfides in good yields. The reaction of acetylenes with 3-methyl-2-buten-1-thio1 gave dihydro-thiophene derivatives in one pot.
Increased aldehyde reductase expression mediates acquired radioresistance of laryngeal cancer cells via modulating p53 (2012)

The main obstacle to cure tumors by radiotherapy has been ascribed to tumor radioresistance. To determine the mechanisms underlying resistance to irradiation, it is essential to compare proteins differentially expressed from radiotherapy-sensitive and -resistant cancer cells. Aldehyde reductase (AKR1A1) was recently identified as increased in radioresistant laryngeal cancer cells by comparative proteomics approach. Here, we provide the mechanism of AKR1A1-mediated radioresistance via p53 regulation in laryngeal cancer cells. AKR1A1 induction was correlated with the radioresistant phenotype of laryngeal cancer HEp-2 cells. AKR1A1 depletion with siRNA significantly enhanced radiation sensitivity of radioresistant HEp-2 cells by promoting radiation-induced cell death and accelerated radiation-mediated inhibition of cell proliferation, without affecting either the PI3K-Akt or MAPK-ERK pathways. Intriguingly, AKR1A1 depletion induced phosphorylation of p53 at serine 15 and G 2/M transition in response to irradiation. We further found that AKR1A1 interacted with p53 and this interaction was dramatically increased in the irradiated radioresistant cells compared with the control cells. AKR1A1 expression also regulated p53 stability in response to irradiation. Furthermore, AKR1A1 depletion only sensitized HCT116 cells expressing p53 to irradiation and not p53-deficient cells. Therefore, our data suggest that radiation-inducible AKR1A1 contributes to acquired radioresistance of laryngeal cancer cells by suppressing p53 activation through inhibitory interaction.
THE GENETIC DEFECT IN ATAXIA-TELANGIECTASIA (1997)

The autosomal recessive human disorder ataxia-telangiectasia (A-T) was first described as a separate disease entity 40 years ago. It is a multisystem disease characterized by progressive cerebellar ataxia, oculocutaneous telangiectasia, radiosensitivity, predisposition to lymphoid malignancies and immunodeficiency, with defects in both cellular and humoral immunity. The pleiotropic nature of the clinical and cellular phenotype suggests that the gene product involved is important in maintaining stability of the genome but also plays a more general role in signal transduction. The chromosomal instability and radiosensitivity so characteristic of this disease appear to be related to defective activation of cell cycle checkpoints. Greater insight into the nature of the defect in A-T has been provided by the recent identification, by positional cloning, of the responsible gene, ATM. The ATM gene is related to a family of genes involved in cellular responses to DNA damage and/or cell cycle control. These genes encode large proteins containing a phosphatidylinositol 3-kinase domain, some of which have protein kinase activity. The mutations causing A-T completely inactivate or eliminate the ATM protein. This protein has been detected and localized to different subcellular compartments.
Designed replacement of an internal hydration water molecule in BPTI: structural and functional implications of a Gly-to-Ser mutation (1993)

The three-dimensional structure of the basic pancreatic trypsin inhibitor (BPTI) contains four internal water molecules, which form a total of nine intermolecular hydrogen bonds with the BPTI polypeptide chain. To investigate the effect of such internal hydration on protein structure and stability, we displaced one of the internal water molecules in a recombinant BPTI analogue, BPTI(G36S), in which Gly 36 is replaced by serine. The replacement of a water molecule by the seryl side chain was established by the absence of the protein-water nuclear Overhauser effects (NOE) that had been attributed to the water molecule near Gly 36 in wild-type BPTI and by the presence of new, intramolecular NOEs to the hydroxyl proton of Ser 36. BPTI(G36S) has slightly reduced thermal stability compared to BPTI, corresponding to a destabilization by delta (delta G) approximately 0.7 kcal/M in 6 M guanidinium hydrochloride solution. Additionally, the stabilities of the complexes formed between BPTI(G36S) and trypsin, plasmin, or kallikrein are significantly reduced when compared to the corresponding complexes with wild-type BPTI.