gebhart/patrepeval huggingface dataset:
Multiple analytical frequencies and standards for the least-squares spectrometric analysis of serum lipids (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMultiple analytical frequencies and standards for the least-squares spectrometric analysis of serum lipidsHarold J. Kisner, Chris W. Brown, and George J. KavarnosCite this: Anal. Chem. 1983, 55, 11, 1703–1707Publication Date (Print):September 1, 1983Publication History Published online1 May 2002Published inissue 1 September 1983https://pubs.acs.org/doi/10.1021/ac00261a014https://doi.org/10.1021/ac00261a014research-articleACS PublicationsRequest reuse permissionsArticle Views106Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
AN ALTERNATIVE APPROACH TO SKIN GRAFT DONOR SITE DRESSING (1986)

A simple closed suction drainage dressing for skin graft donor sites, prepared from readily available items, is described.
Process Scale Purification of Antibodies (2008)

Process scale purification of antibodies , Process scale purification of antibodies , کتابخانه مرکزی دانشگاه علوم پزشکی تهران
Role of lysine, tryptophan and calcium in the β-elimination activity of a low-molecular-mass pectate lyase from Fusarium moniliformae (1996)

An extracellular pectate lyase from Fusarium moniliformae was purified to homogeneity by affinity chromatography followed by gel filtration, with a yield of 76.5%. Laser desorption MS of the enzyme gave a molecular mass of 12,133.5 +/- 2.5 Da. The pectate lyase was a glycoprotein with a 5% carbohydrate content and had a pl value of 9.1. Atomic-emission spectrometry showed that Ca2+ was a part of the holoenzyme held by carboxy groups of the protein. These results support the hypothesis of a putative Ca2+ site suggested by Yodder, Keen and Jurnak [(1993) Science 260, 1503-1507] in the crystal structure of pectate lyase C of Erwinia chrysanthemi. Loss of Ca2+ was observed by treatment with EGTA or carboxy-modifying Woodward's reagent K, with subsequent loss of enzyme activity. Tryptophan fluorescence quenching showed that Ca2+ does not affect binding of substrate to enzyme. Chemical-modification and substrate-protection studies showed the presence of lysine and tryptophan at or near the active site of the pectate lyase. Chemically modified enzyme showed no major structural changes as determined by CD. Amino acid analyses of native, trinitrobenzenesulphonate (TBNS)-treated and substrate-protected TNBS-treated enzyme showed that a single essential residue of lysine is present at or near the active-site. Substrate-affinity studies showed that tryptophan could be essential for substrate binding, whereas lysine could be involved in the catalysis. Fluorescence quenching further confirmed the involvement of tryptophan in substrate binding. The reaction mechanism involving beta-elimination by this enzyme is discussed.
In vitro and in vivo modifications of recombinant and human IgG antibodies (2014)

Tremendous knowledge has been gained in the understanding of various modifications of IgG antibodies, driven mainly by the fact that antibodies are one of the most important groups of therapeutic molecules and because of the development of advanced analytical techniques. Recombinant monoclonal antibody (mAb) therapeutics expressed in mammalian cell lines and endogenous IgG molecules secreted by B cells in the human body share some modifications, but each have some unique modifications. Modifications that are common to recombinant mAb and endogenous IgG molecules are considered to pose a lower risk of immunogenicity. On the other hand, modifications that are unique to recombinant mAbs could potentially pose higher risk. The focus of this review is the comparison of frequently observed modifications of recombinant monoclonal antibodies to those of endogenous IgG molecules.
Structural transitions during prothrombin activation: On the importance of fragment 2 (2015)

Prothrombin is activated to thrombin by the prothrombinase complex through sequential cleavage at two distinct sites. This occurs at sites of vascular injury in a highly regulated cascade of serine protease and cofactor activation, where activated platelets provide a suitable surface for protease/cofactor/substrate assembly. The precise structural and conformational changes undergone during the transition from prothrombin to thrombin have been studied for decades, and several structures of prothrombin fragments along the activation pathway have been solved. Here we present a new structure analyzed in context of other recent structures and biochemical studies. What emerges is an unexpected mechanism that involves a change in the mode of binding of the F2 domain (fragment 2) on the catalytic domain after cleavage at Arg320, and a subsequent reorientation of the linker between the F2 and catalytic domain to present the Arg271 site for cleavage.
Diagnosis of head-and-neck cancer from exhaled breath (2011)

The presented results could lead to the development of a cost-effective, fast, and reliable method for the differential diagnosis of HNC that is based on breath testing with an NA-NOSE, with a future potential as screening tool.
Fate of H2 in an Upflow Single-Chamber Microbial Electrolysis Cell Using a Metal-Catalyst-Free Cathode (2009)

With the goal of maximizing the H2-harvesting efficiency, we designed an upflow single-chamber microbial electrolysis cell (MEC) by placing the cathode on the top of the MEC and carried out a program to track the fate of H2 and electron equivalents in batch experiments. When the initial acetate concentration was 10 mM in batch-evaluation experiments lasting 32 h, the cathodic conversion efficiency (CCE) from coulombs (i.e., electron equivalents in current from the anode to the cathode) to H2 was 98 +/- 2%, the Coulombic efficiency (CE) was 60 +/- 1%, the H2 yield was 59 +/- 2%, and methane production was negligible. However, longer batch reaction time (approximately 7 days) associated with higher initial acetate concentrations (30 or 80 mM) led to significant H2 loss due to CH4 accumulation: up to 14 +/- 1% and 16 +/- 2% of the biogas at 30 and 80 mM of acetate, respectively. Quantitative PCR proved that no acetoclastic methanogens were present, but that hydrogenotrophic methanogens (i.e., Methanobacteriales) were present on both electrodes. The hydrogenotrophic methanogens decreased the CCE by diverting H2 generated at the cathode to CH4 in the upflow single-chamber MEC. In some experiments, the CE was greater than 100%. The cause was anode-respiring bacteria oxidizing H2 and producing current which recycled H2 between the cathode and the anodes, increasing CE to over 100%, but with a concomitant decline in CCE, despite negligible CH4 formation.
Urea denaturation of α‐hemolysin pore inserted in planar lipid bilayer detected by single nanopore recording: Loss of structural asymmetry (2007)

The aim of this work is to study pore protein denaturation inside a lipid bilayer and to probe current asymmetry as a function of the channel conformation. We describe the urea denaturation of alpha-hemolysin channel and the channel formation of alpha-hemolysin monomer incubated with urea prior to insertion into a lipid bilayer. Analysis of single-channel recordings of current traces reveals a sigmoid curve of current intensity as a function of urea concentration. The normalized current asymmetry at 29+/-4% is observed between 0 and 3.56M concentrations and vanishes abruptly down to 0 concentration exceeds 4M. The loss of current asymmetry through alpha-hemolysin is due to the denaturation of the channel's cap. We also show that the alpha-hemolysin pore inserted into a lipid bilayer is much more resistant to urea denaturation than the alpha-hemolysin monomer in solution: The pore remains in the lipid bilayer up to 7.2M urea. The pore formation is possible up to 4.66M urea when protein monomers were previously incubated in urea.
A Lethal Toxic Substance for Brewing Yeast in Wheat and Barley (1970)

A toxic substance contained in wheat and barley causing death of brewing yeast was purified and isolated as a single protein. The molecular weight of the toxic substance was estimated to be 9800 by Archibald method. The substance was found to be a simple protein by amino acid analysis. The isoelectric point was higher than pH 10. The UV absorption spectrum showed λmax 278mμ. By the measurement of the circular dichroic spectrum, the α-helix content was estimated to be 34.5%. The toxic effect was easily inactivated by trypsin, but not by chymotrypsin and carboxypeptidase.
Molecular profiling of CD3−CD4+ T cells from patients with the lymphocytic variant of hypereosinophilic syndrome reveals targeting of growth control pathways (2009)

The clonal CD3(-)CD4(+) T-cell population characterizing lymphocytic variant hypereosinophilic syndrome (L-HES) persists for years, with a subgroup of patients ultimately progressing to T lymphoma. The molecular changes associated with the premalignant clone and the emergence of malignant subclones are unknown, precluding the development of targeted therapy for this HES variant. In this study, we used whole genome arrays to examine gene expression in the CD3(-)CD4(+) T cells and found that 850 genes were differentially regulated during chronic disease compared with CD3(+)CD4(+) T cells from healthy donors. Changes in the expression of 349 genes were altered in association with the clinical progression from chronic L-HES to T lymphoma in 1 patient, with 87 of 349 genes representing further changes in genes whose expression was altered in all chronic disease patients (87 of 850). Array analysis after CD2/CD28-mediated activation revealed that the major gene expression changes observed in the CD3(-)CD4(+) T cells do not reflect activation induced alterations but rather pathways involved in T-cell homeostasis, including transforming growth factor-beta signaling, apoptosis, and T-cell maturation, signaling, and migration. Examination of microRNA expression in the CD3(-)CD4(+) T cells from patients with chronic disease identified 23 microRNAs that changed significantly, among which miR-125a further decreased in association with one patient's evolution to T lymphoma.
Long-Term IGF-I Exposure Decreases Autophagy and Cell Viability (2010)

A reduction in IGF-I signaling has been found to increase lifespan in multiple organisms despite the fact that IGF-I is a trophic factor for many cell types and has been found to have protective effects against multiple forms of damage in acute settings. The increase in longevity seen in response to reduced IGF-I signaling suggests that there may be differences between the acute and chronic impact of IGF-I signaling. We have examined the possibility that long-term stimulation with IGF-I may have a negative impact at the cellular level using quiescent human fibroblasts. We find that fibroblast cells exposed to IGF-I for 14 days have reduced long-term viability as judged by colony forming assays, which is accompanied by an accumulation of senescent cells. In addition we observe an accumulation of cells with depolarized mitochondria and a reduction in autophagy in the long-term IGF-I treated cultures. An examination of mice with reduced IGF-I levels reveals evidence of enhanced autophagy and fibroblast cells derived from these mice have a larger mitochondrial mass relative to controls indicating that changes in mitochondrial turnover occurs in animals with reduced IGF-I. The results indicate that chronic IGF-I stimulation leads to mitochondrial dysfunction and reduced cell viability.
Tolerance of Skin Homografts Induced in Adult Mice by Multiple Injections of Homologous Spleen Cells. (1961)

1. Adult mice of the Z(C3H) strain have been made tolerant of skin homo-grafts of the(A X Z) F1 hybrid strain by repeated intravenous and intraperitoneal injections of homologous spleen cells. 2. Repeated intraperitoneal injections of homologous spleen cells generally failed to induce a tolerant state, although 3/75 recipients accepted the homologous skin homotransplant.
Doppelgänger goes to school : machine learning for user modeling (1993)

Thesis (M.S.)--Massachusetts Institute of Technology, Program in Media Arts & Sciences, 1993.
Characterization of a New NIH-Registered Variant Human Embryonic Stem Cell Line, BG01V: A Tool for Human Embryonic Stem Cell Research (2005)

Abstract Human embryonic stem cells (hESCs) offer a renewable source of a wide range of cell types for use in research and cell-based therapies. Characterizing these cells provides important information about their current state and affords relevant details for subsequent manipulations. For example, identifying genes expressed during culture, as well as their temporal expression order after passaging and conditions influencing the formation of all three germ layers may be helpful for the production of functional beta islet cells used in treating type I diabetes. Although several hESC lines have demonstrated karyotypic instability during extended time in culture, select variant lines exhibit characteristics similar to their normal parental lines. Such variant lines may be excellent tools and abundant sources of cells for pilot studies and in vitro differentiation research in which chromosome number is not a concern, similar to the role currently played by embryonal carcinoma cell lines. It is crucial that the cells be surveyed at a genetic and proteomic level during extensive propagation, expansion, and manipulation in vitro. Here we describe a comprehensive characterization of the variant hESC line BG01V, which was derived from the karyotypically normal, parental hESC line BG01. Our characterization process employs cytogenetic analysis, short tandem repeat and HLA typing, mitochondrial DNA sequencing, gene expression analysis using quantitative reverse transcription-polymerase chain reaction and microarray, assessment of telomerase activity, methylation analysis, and immunophenotyping and teratoma formation, in addition to screening for bacterial, fungal, mycoplasma, and human pathogen contamination.
One-Pot Synthesis of 2,3-Disubstituted N-Tosylindoles from o-Acyl-N-tosylanilines (2006)

The reaction of o-acyl-N-tosylanilines with lithium tri­methylsilyldiazomethane followed by treatment with t-BuLi and then electrophiles gave 2,3-disubstituted N-tosylindoles in a one-pot process.
I Am So Big (1963)

I am so big. My footsteps span the galaxies when I walk. I can pick a star cluster and set it on my fingernail as you do with the fireflies that fly the summer nights...
Endovaginal Ultrasound to Exclude Endometrial Cancer and Other Endometrial Abnormalities (1998)

Endovaginal ultrasound has a high sensitivity for detecting endometrial cancer and other endometrial disease and can reliably identify postmenopausal women with vaginal bleeding who are highly unlikely to have significant endometrial disease so that endometrial sampling may be unnecessary.
Development of a Production-Ready, Back-Illuminated CMOS Image Sensor with Small Pixels (2007)

A back-illuminated 2 megapixel CMOS sensor utilizing mature wafer manufacturing operations is described. Sensitivity, dark current and other key pixel performance measures are compared against an equivalent conventional sensor. Aspects of the process integration that make the technology manufacturable are described. Simulations that predict the performance of a full color sensor are discussed.
The role of RAGE in amyloid-beta peptide-mediated pathology in Alzheimer's disease. (2009)

This review discusses current knowledge of the complex interactions between amyloid-beta (A beta) peptide, the receptor for advanced glycation endproducts (RAGE), and inflammatory mediators, focusing on the roles of such interactions in the pathogenesis of Alzheimer's disease. As a ubiquitous cell-surface receptor, RAGE demonstrates enhanced expression in an A beta-rich environment; the effects of RAGE on microglia, the blood-brain barrier and neurons are mediated through various signaling pathways. Relevant preclinical models illustrate that the A beta-RAGE interaction amplifies neuronal stress and the accumulation of A beta, impairs memory and learning, and exaggerates neuroinflammation. These findings suggest that RAGE may mediate a common proinflammatory pathway in neurodegenerative disorders.
Methods for the detection and enumeration of Alicyclobacillus acidoterrestris and investigation of growth and production of taint in fruit juice and fruit juice‐containing drinks (1997)

Methods for the detection of Alicyclobacillus acidoterrestris at a level of 1 cell per 100 ml and enumeration with a sensitivity of 5 cells ml-1 were developed. Spores of A. acidoterrestris survived pasteurization and outgrew and multiplied at a similar rate to vegetative cells in both orange juice and apple juice. Alicyclobacillus acidoterrestris grew readily in orange juice, apple juice and a non-carbonated fruit juice-containing drink at temperatures of 25-44 degrees C producing a taint and elevated levels (1-100 ppb) of guaiacol. Isolates of A. acidoterrestris can be identified using the DuPont RiboPrinter. It was isolated from apple drinks, apple juice concentrate and freshly squeezed orange juice.
Isolation and characterization of a surfactant produced byBacillus licheniformis 86 (1990)

Surfactant (BL86) was isolated from foam produced during growth of Bacillus licheniformis 86 by acid-precipitation followed by extraction into tetrahydrofuran or methanol. The surfaetant is anionic and dissolves in tetrahydrofuran, methanol, chloroform, dichloromethane, xylene, toluene, and alkaline water. The surfactant lowers the surface tension of water to 27 dynes/cm, and achieves the critical micelle concentration with as little as 10/~g surfactant/ml. Its interfacial tension can reach 0.36 dynes/cm when measured in 4~ sodium chloride against n-hexadecane. The surfactant is stable from pH 4.0 to 13.0, at temperatures ranging from 25 to 120 ~ and in salt solutions ranging from 0 to 30~ NaC1. Preliminary analytical results indicate that the surfactant is a mixture of lipopeptides different from previously reported Bacillus produced surfactants.
And the Winner Is ... Trial Lawyers: When Does an Accommodation under Title II of the ADA Represent a Fundamental Alteration of Competitive Sports (2002)

Prior to the United State Supreme Court’s decision in PGA Tour, Inc. v. Martin, fans could rest comfortably knowing that their games would stay true to the traditions they hold dear. After the decision, however, it appears that no rule, no matter how well established, is safe from a judicial determination that the rule is not fundamental and, thus, may be altered to accommodate individuals with disabilities. In PGA Tour, the Supreme Court forever changed when a proposed modification in competitive sports will be denied based on the fundamental alteration defense under Title III of the Americans with Disabilities Act. With the decision, the Supreme Court opened the floodgates for possible litigation while hurting both competitive sports and disabled athletes.
Wnt-Responsive Lgr5-Expressing Stem Cells Are Hair Cell Progenitors in the Cochlea (2012)

Auditory hair cells are surrounded on their basolateral aspects by supporting cells, and these two cell types together constitute the sensory epithelium of the organ of Corti, which is the hearing apparatus of the ear. We show here that Lgr5, a marker for adult stem cells, was expressed in a subset of supporting cells in the newborn and adult murine cochlea. Lgr5-expressing supporting cells, sorted by flow cytometry and cultured in a single-cell suspension, compared with unsorted cells, displayed an enhanced capacity for self-renewing neurosphere formation in response to Wnt and were converted to hair cells at a higher (>10-fold) rate. The greater differentiation of hair cells in the neurosphere assay showed that Lgr5-positive cells had the capacity to act as cochlear progenitor cells, and lineage tracing confirmed that Lgr5-expressing cells accounted for the cells that formed neurospheres and differentiated to hair cells. The responsiveness to Wnt of cells with a capacity for division and sensory cell formation suggests a potential route to new hair cell generation in the adult cochlea.
What Is an ABI (2010)

The concept of acquired brain injury (ABI) and its causes are discussed. Various efforts are been undertaken by several associations such as Brain Injury Australia to increase community understanding and awareness, so that people with ABI are not socially isolated.
Roles for endocytic trafficking and phosphatidylinositol 4-kinase III alpha in hepatitis C virus replication (2009)

Hepatitis C virus (HCV) reorganizes cellular membranes to establish sites of replication. The required host pathways and the mechanism of cellular membrane reorganization are poorly characterized. Therefore, we interrogated a customized small interfering RNA (siRNA) library that targets 140 host membrane-trafficking genes to identify genes required for both HCV subgenomic replication and infectious virus production. We identified 7 host cofactors of viral replication, including Cdc42 and Rock2 (actin polymerization), EEA1 and Rab5A (early endosomes), Rab7L1, and PI3-kinase C2gamma and PI4-kinase IIIalpha (phospholipid metabolism). Studies of drug inhibitors indicate actin polymerization and phospholipid kinase activity are required for HCV replication. We found extensive co-localization of the HCV replicase markers NS5A and double-stranded RNA with Rab5A and partial co-localization with Rab7L1. PI4K-IIIalpha co-localized with NS5A and double-stranded RNA in addition to being present in detergent-resistant membranes containing NS5A. In a comparison of type II and type III PI4-kinases, PI4Ks were not required for HCV entry, and only PI4K-IIIalpha was required for HCV replication. Although PI4K-IIIalpha siRNAs decreased HCV replication and virus production by almost 100%, they had no effect on initial HCV RNA translation, suggesting that PI4K-IIIalpha functions at a posttranslational stage. Electron microscopy identified the presence of membranous webs, which are thought to be the site of HCV replication, in HCV-infected cells. Pretreatment with PI4K-IIIalpha siRNAs greatly reduced the accumulation of these membranous web structures in HCV-infected cells. We propose that PI4K-IIIalpha plays an essential role in membrane alterations leading to the formation of HCV replication complexes.