gebhart/patrepeval huggingface dataset:
Potential Purine Antagonists. IV. Synthesis of Some 9-Methyl-6-substituted-purines1 (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotential Purine Antagonists. IV. Synthesis of Some 9-Methyl-6-substituted-purines1Roland K. Robins and Hsi Hu LinCite this: J. Am. Chem. Soc. 1957, 79, 2, 490–494Publication Date (Print):January 1, 1957Publication History Published online1 May 2002Published inissue 1 January 1957https://pubs.acs.org/doi/10.1021/ja01559a069https://doi.org/10.1021/ja01559a069research-articleACS PublicationsRequest reuse permissionsArticle Views368Altmetric-Citations72LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Diverse Roles for Sex Hormone-Binding Globulin in Reproduction (2011)

Sex hormone-binding globulin (SHBG) transports androgens and estrogens in blood and regulates their access to target tissues. Hepatic production of SHBG fluctuates throughout the life cycle and is influenced primarily by metabolic and hormonal factors. Genetic differences also contribute to interindividual variations in plasma SHBG levels. In addition to controlling the plasma distribution, metabolic clearance, and bioavailability of sex steroids, SHBG accumulates in the extravascular compartments of some tissues and in the cytoplasm of specific epithelial cells, where it exerts novel effects on androgen and estrogen action. In mammals, the gene-encoding SHBG is expressed primarily in the liver but also at low levels in other tissues, including the testis. In subprimate species, Shbg expression in Sertoli cells is under the control of follicle-stimulating hormone and produces the androgen-binding protein that influences androgen actions in the seminiferous tubules and epididymis. In humans, the SHBG gene is not expressed in Sertoli cells, but its expression in germ cells produces an SHBG isoform that accumulates in the acrosome. In fish, Shbg is produced by the liver but has a unique function in the gill as a portal for natural steroids and xenobiotics, including synthetic steroids. However, salmon have retained a second, poorly conserved Shbg gene that is expressed only in ovary, muscle, and gill and that likely exerts specialized functions in these tissues. The present review compares the production and functions of SHBG in different species and its diverse effects on reproduction.
Apical and basolateral coated pits of MDCK cells differ in their rates of maturation into coated vesicles, but not in the ability to distinguish between mutant hemagglutinin proteins with different internalization signals. (1995)

In polarized epithelial MDCK cells, all known endogenous endocytic receptors are found on the basolateral domain. The influenza virus hemagglutinin (HA) which is normally sorted to the apical plasma membrane, can be converted to a basolateral protein by specific mutations in its short cytoplasmic domain that also create internalization signals. For some of these mutations, sorting to the basolateral surface is incomplete, allowing internalization of two proteins that differ by a single amino acid of the internalization signal to be compared at both the apical and basolateral surfaces of MDCK cells. The rates of internalization of HA-Y543 and HA-Y543,R546 from the basolateral surface of polarized MDCK cells resembled those in nonpolarized cells, whereas their rates of internalization from the apical cell surface were fivefold slower. However, HA-Y543,R546 was internalized approximately threefold faster than HA-Y543 at both membrane domains, indicating that apical endocytic pits in polarized MDCK cells retained the ability to discriminate between different internalization signals. Slower internalization from the apical surface could not be explained by a limiting number of coated pits: apical membrane contained 0.7 as many coated pits per cell cross-section as did basolateral membranes. 10-14% of HA-Y543 at the apical surface of polarized MDCK cells was found in coated pits, a percentage not significantly different from that observed in apical coated pits of nonpolarized MDCK cells, where internalization was fivefold faster. Thus, there was no lack of binding sites for HA-Y543 in apical coated pits of polarized cells. However, at the apical surface many more shallow pits, and fewer deep, mature pits, were observed than were seen at the basolateral. These results suggest that the slower internalization at the apical surface is due to slower maturation of coated pits, and not to a difference in recognition of internalization signals.
Induction of plasticity in the human motor cortex by paired associative stimulation (2000)

Current models of motor cortical plasticity, developed in studies on experimental animals, emphasize the importance of the conjoint activity of somatosensory afferents and intrinsic motor cortical circuits. The hypothesis that an enduring change in excitability in the cortical output circuitry can be induced in the human motor cortex by a paired-stimulation protocol was tested. Low-frequency median nerve stimulation was paired with transcranial magnetic stimulation (TMS) over the optimal cranial site for stimulating the abductor pollicis brevis muscle (APB). This protocol induced an increase in the amplitudes of the motor evoked potentials (MEPs) in the resting APB as well as a prolongation of the silent period measured in the precontracted APB following TMS; amplitudes of MEPs measured in voluntary contraction remained unchanged. Experiments testing the excitability of spinal motoneurons using F-wave studies and electrical stimulation of the brainstem suggested that the site of the plastic changes was within the motor cortex. The increases in resting amplitudes and silent period duration were conditionally dependent on the timing between the afferent and the magnetic stimulation in that they were present when events elicited by afferent and magnetic stimulation were synchronous at the level of the motor cortex. Plasticity induced by paired stimulation evolved rapidly (within 30 min), was persistent (minimum duration 30-60 min) yet reversible, and was topographically specific. This combination of features and the similarity to properties of induced enduring changes in synaptic efficacy, as elucidated in animal studies, leads us to propose that the induced plasticity may represent a signature of associative long-term potentiation of cortical synapses or closely related neuronal mechanisms in the human cortex.
The Ability of Variant Peptides to Reverse the Nonresponsiveness of T Lymphocytes to the Wild-Type Sequence p53264–272 Epitope (2002)

Recently, we observed that CTL specific for the wild-type (wt) sequence p53(264-272) peptide could only be expanded ex vivo from PBMC of a subset of the HLA-A2.1(+) normal donors or cancer patients tested. Surprisingly, the tumors of the responsive patients expressed normal levels of wt p53 and could be considered unlikely to present this epitope. In contrast, tumors of nonresponsive patients accumulated mutant p53 and were more likely to present this epitope. We sought to increase the responsive rate to the wt p53(264-272) peptide of PBMC obtained from normal donors and patients by identifying more immunogenic variants of this peptide. Two such variants were generated by amino acid exchanges at positions 6 (6T) and 7 (7W) of the peptide. These variants were capable of inducing T cells from PBMC of nonresponsive donors that recognized the parental peptide either pulsed onto target cells or naturally presented by tumors. TCR Vbeta analysis of two T cell lines isolated from bulk populations of effectors reactive against the wt p53(264-272) peptide, using either the parental or the 7W variant peptide, indicated that these T cells were expressing identical TCR Vbeta13.6/complementarity-determining region 3/J region sequences. This finding confirms the heteroclitic nature of at least one of the variant peptides identified in this study. The use of variant peptides of the wt p53(264-272) epitope represents a promising approach to overcoming the nonresponsiveness of certain cancer patients to this self epitope, thereby enhancing its potential use in tumor vaccines for appropriately selected cancer patients.
ε Protein Kinase C in Pathological Myocardial Hypertrophy (2000)

The epsilon isoform of protein kinase C (PKC) has a critical cardiotrophic function in normal postnatal developing heart as demonstrated by cardiac-specific transgenic expression of epsilonPKC-selective translocation inhibitor (epsilonV1) and activator (psiepsilonRACK) peptides (Mochly-Rosen, D., Wu, G., Hahn, H., Osinska, H., Liron, T., Lorenz, J. N., Robbins, J., and Dorn, G. W., II (2000) Circ. Res. 86, 1173-1179). To define the role of epsilonPKC signaling in pathological myocardial hypertrophy, epsilonV1 or psiepsilonRACK were co-expressed in mouse hearts with Galpha(q), a PKC-linked hypertrophy signal transducer. Compared with Galpha(q) overexpression alone, co-expression of psiepsilonRACK with Galpha(q) increased epsilonPKC particulate partitioning by 30 +/- 2%, whereas co-expression of epsilonV1 with Galpha(q) reduced particulate-associated epsilonPKC by 22 +/- 1%. Facilitation of epsilonPKC translocation by psiepsilonRACK in Galpha(q) mice improved cardiac contractile function measured as left ventricular fractional shortening (30 +/- 3% Galpha(q) versus 43 +/- 2% psiepsilonRACK/Galpha(q), p < 0.05). Conversely, inhibition of epsilonPKC by epsilonV1 modified the Galpha(q) nonfailing hypertrophy phenotype to that of a lethal dilated cardiomyopathy. These opposing effects of epsilonPKC translocation activation and inhibition in Galpha(q) hypertrophy indicate that epsilonPKC signaling is a compensatory event in myocardial hypertrophy, rather than a pathological event, and support the possible therapeutic efficacy of selective epsilonPKC translocation enhancement in cardiac insufficiency.
Antioxidant Peptidomics Reveals Novel Skin Antioxidant System (2008)

It is generally agreed that reactive oxygen species (ROS) contribute to skin aging, skin disorders, and skin diseases. Skin possesses an extremely efficient antioxidant system. This antioxidant activity is conferred by two systems: antioxidant enzymes and small molecules that can scavenge ROS by donating electrons. No gene-encoded secreted ROS scavengers have been reported. Amphibian skin is a multifunctional organ acting in defense, respiration, and water regulation, although it seems susceptible. Amphibian skins are easily harmed by biological or non-biological attacks such as microorganism infection or radiation injury. Among vertebrates, skins of amphibian are exposed to more dangers of radiation injury than others. Radiation toxicity occurs by directly attacking the genetic material and/or by generating ROS. In addition, amphibian skin respiration and inflammatory response also induce ROS generation. It is rational to hypothesize that amphibian skins should have potent free radical scavenging and radioprotective ability for their survival. Rana pleuraden is distributed in Southwest of China; it lives in the subtropical plateau (altitude around 2300 m) where there is strong ultraviolet radiation and long duration of sunshine. By peptidomics and genomics approaches, a large amount of antioxidant peptides belonging to 11 different groups with variable structures were isolated from the skin secretions of R. pleuraden. Their free radical scavenging and anti-inflammatory abilities were studied. All of these peptide share highly homologous preproregions, although mature antioxidant peptides have very divergent primary structures, suggesting the possibility of a common ancestor. Some peptides were also found to have multifunctional properties, such as combined antioxidant, anti-inflammatory, and antimicrobial activities. According to our knowledge, no gene-encoded specific antioxidant peptides have been reported except metallothionein. Our work possibly reveals a new skin antioxidant system. The current work also provides a large amount of peptide candidates with medical-pharmaceutical significance.
Feasibility and Tolerability of Sequential Doxorubicin/Paclitaxel Followed by Cyclophosphamide, Methotrexate, and Fluorouracil and Its Effects on Tumor Response as Preoperative Therapy (2005)

Doxorubicin/paclitaxel-->CMF is feasible, safe, and well tolerated. Given as PST, it is markedly active, allowing for breast-sparing surgery in a large fraction of patients.
ADRENAL MEDULLARY CATECHOLAMINE SECRETION PATTERNS IN RATS EVOKED BY REFLEX AND DIRECT NEURAL STIMULATION (2000)

Epinephrine (EPI) and norepinephrine (NE), secretion patterns evoked by reflex (to hypotension and hypoglycemia) and direct neural stimulation of the adrenal medulla were measured in pentobarbital anesthetized male Sprague-Dawley rats. Secretion rates were determined by collecting adrenal venous blood. Baseline catecholamine secretion was similar in innervated and denervated glands indicating that there was little tonic sympathetic neural drive to the medulla. Both hydralazine-induced hypotension and insulin-induced hypoglycemia significantly increased catecholamine secretion with the secretion of EPI predominating. Similarly, in response to stimulation of the splanchnic nerve, frequency-related increments in EPI and NE were elicited with EPI release being greater than NE at all frequencies. However, the magnitude of the increase in secretion during splanchnic stimulation at frequencies above 1 hz greatly exceeded the release achieved by reflex stimulation. The results indicate that despite the fact that the stimuli of hypotension and hypoglycemia are integrated by different centers in the brain, the pattern of adrenal release is similar.
Enhanced targeting with heterobivalent ligands (2009)

A novel approach to specifically target tumor cells for detection and treatment is the proposed use of heteromultivalent ligands, which are designed to interact with, and noncovalently crosslink, multiple different cell surface receptors. Although enhanced binding has been shown for synthetic homomultivalent ligands, proof of cross-linking requires the use of ligands with two or more different binding moieties. As proof-of-concept, we have examined the binding of synthetic heterobivalent ligands to cell lines that were engineered to coexpress two different G-protein-coupled human receptors, i.e., the human melanocortin 4 receptor (MC4R) expressed in combination with either the human delta-opioid receptor (deltaOR) or the human cholecystokinin-2 receptor (CCK2R). Expression levels of these receptors were characterized by time-resolved fluorescence saturation binding assays using Europium-labeled ligands; Eu-DPLCE, Eu-NDP-alpha-MSH, and Eu-CCK8 for the deltaOR, MC4R, and CCK2R, respectively. Heterobivalent ligands were synthesized to contain a MC4R agonist connected via chemical linkers to either a deltaOR or a CCK2R agonist. In both cell systems, the heterobivalent constructs bound with much higher affinity to cells expressing both receptors, compared with cells with single receptors or to cells where one of the receptors was competitively blocked. These results indicate that synthetic heterobivalent ligands can noncovalently crosslink two unrelated cell surface receptors, making feasible the targeting of receptor combinations. The in vitro cell models described herein will lead to the development of multivalent ligands for target combinations identified in human cancers.
Femtosecond Imaging of Surface Plasmon Dynamics in a Nanostructured Silver Film (2005)

Light interacting with nanostructured metals excites the collective charge density fluctuations known as surface plasmons (SP). Through excitation of the localized SP eigenmodes incident light is trapped on the nanometer spatial and femtosecond temporal scales and its field is enhanced. Here we demonstrate the imaging and quantum control of SP dynamics in a nanostructured silver film. By inducing and imaging the nonlinear two-photon photoemission from the sample with a pair of identical 10-fs laser pulses while scanning the pulse delay, we record a movie of SP fields at a rate of 330-attoseconds/frame.
Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin (2008)

Adipose-derived stem cells (ASCs) are promising candidates for therapy in myocardial infarction (MI). However, the frequency of human ASCs that differentiate towards cardiomyocytes is low. We hypothesized that adherence to extracellular matrix molecules that are upregulated after MI might increase human stem cell differentiation towards cardiomyocytes. We analysed putative ASC differentiation on fibronectin-coated, laminin-coated and uncoated culture plates. Expression of cardiac markers in cells was analysed 1, 3 and 5 weeks after stimulation with 5-aza-2-deoxycytidine. After 1 week, mRNA expression of myosin light chain-2alpha (MLC-2alpha), an early marker in cardiomyocyte development, was increased significantly in treated cells, independent of coating. At 5 weeks, however, mRNA expression of the late cardiomyocyte development marker SERCA2alpha was only significantly increased in 5-aza-2-deoxycytidine-treated cells cultured on laminin. Significantly higher numbers of cells were immunopositive for MLC-2alpha in cultures of treated cells grown on laminin-coated wells, when compared with cultures of treated cells grown on uncoated wells, both at 1 week and at 5 weeks. Furthermore, after 3 weeks, significantly more alpha-actinin- and desmin-positive cells were detected after treatment with 5-aza-2-deoxycytidine, but only in uncoated wells. After 5 weeks, however, the number of desmin-positive cells was only significantly increased after treatment of cells with 5-aza-2-deoxycytidine and culture on laminin (61% positive cells). Thus, we have found that a high percentage of human ASCs can be differentiated towards cardiomyocytes; this effect can be improved by laminin, especially during late differentiation.
Reversible Hydrophobic–Hydrophilic Transition of Ionic Liquids Driven by Carbon Dioxide (2015)

Ionic liquids (ILs) with a reversible hydrophobic-hydrophilic transition were developed, and they exhibited unique phase behavior with H2O: monophase in the presence of CO2, but biphase upon removal of CO2 at room temperature and atmospheric pressure. Thus, coupling of reaction, separation, and recovery steps in sustainable chemical processes could be realized by a reversible liquid-liquid phase transition of such IL-H2O mixtures. Spectroscopic investigations and DFT calculations showed that the mechanism behind hydrophobic-hydrophilic transition involved reversible reaction of CO2 with anion of the ILs and formation of hydrophilic ammonium salts. These unique IL-H2O systems were successfully utilized for facile one-step synthesis of Au porous films by bubbling CO2 under ambient conditions. The Au porous films and the ILs were then separated simultaneously from aqueous solutions by bubbling N2, and recovered ILs could be directly reused in the next process.
High-Power (over 100 mW) Green Laser Diodes on Semipolar $\{20\bar{2}1\}$ GaN Substrates Operating at Wavelengths beyond 530 nm (2012)

Continuous-wave operation of InGaN green laser diodes (LDs) on semipolar {2021} GaN substrates with output powers of over 100 mW in the spectral region beyond 530 nm is demonstrated. Wall plug efficiencies (WPEs) as high as 7.0–8.9% are realized in the wavelength range of 525–532 nm, which exceed those reported for c-plane LDs. The longest lasing wavelength has reached 536.6 nm under cw operation. These results suggest that the InGaN green LDs on the {2021} plane are better suited as light sources for applications requiring wavelengths over 525 nm.
INTERFACIAL PHENOMENA (Equilibrium and Dynamic Effects) (1986)

FUNDAMENTALS OF INTERFACIAL TENSION Introduction to Interfacial Phenomena Interfacial Tension: Qualitative Considerations Interfacial Tension: Thermodynamic Approach Interfacial Tension: Mechanical Approach Density and Concentration Profiles Equilibrium Shapes of Fluid Interfaces Methods of Measuring Interfacial Tension Surface Tension of Binary Mixtures Surfactants Solid-Fluid Interfaces FUNDAMENTALS OF WETTING, CONTACT ANGLE, AND ADSORPTION Young's Equation Work of Adhesion and Work of Cohesion Phenomenological Theories of Equilibrium Contact Angles Acid-Base Interaction Contact Angle Hysteresis Adsorption Density Profiles in Liquid Films on Solids Characterizing Solid Surfaces COLLOIDAL DISPERSIONS Attractive Forces Electrical Interaction Colloids of All Shapes and Sizes Combined Attractive and Electrical Interaction: DLVO Theory Effect of Polymer Molecules on the Stability of Colloidal Dispersions Kinetics of Coagulation SURFACTANTS Micelle Formation Variation of CMC for Pure Surfactants and Surfactant Mixtures Other Phases Involving Surfactants Formation of Complexes Between Surfactants and Polymers Surface Films of Insoluble Substrates Solubilization and Microemulsions Phase Behavior and Interfacial Tension for Oil-Water-Surfactant Systems Effect of Composition Changes Thermodynamics of Microemulsions Applications of Surfactants: Emulsions Applications of Surfactants: Detergency Chemical Reactions in Micellar Solutions and Microemulsions INTERFACES IN MOTION: STABILITY AND WAVE MOTION Linear Analysis of Interfacial Stability Damping of Capillary Wave Motion by Insoluble Surfactants Instability of Fluid Cylinders or Jets Oscillating Jet Stability and Wave Motion of Thin Liquid Films: Foams Energy and Force Methods for Thermodynamic Stability of Interfaces Interfacial Stability for Fluids in Motion: Kelvin-Helmholtz Instability Waves on a Falling Liquid Film TRANSPORT EFFECTS ON INTERFACIAL PHENOMENA Interfacial Tension Variation Interfacial Species Mass Balance and Energy Balance Interfacial Instability for a Liquid Heated from Below or Cooled from Above Interfacial Instability During Mass Transfer Other Phenomena Influenced by Marangoni Flow Nonequilibrium Interfacial Tensions Effect of Surfactant Transport on Wave Motion Stability of Moving Interfaces with Phase Transformation Stability of Moving Interfaces with Chemical Reaction Intermediate Phase Formation Transport-Related Spontaneous Emulsification Interfacial Mass Transfer Resistance Other Interfacial Phenomena Involving Dispersed Phase Formation DYNAMIC INTERFACES Surfaces Basic Equations of Fluid Mechanics Flow Past a Droplet Asymptotic Analysis Dip Coating Spherical Drop Revisited Surface Rheology Drainage of Thin Liquid Films Dynamic Contact Lines Slip Thin and Ultrathin Films SIZE, SHAPE, STRUCTURE, DIFFUSIVITY, AND MASS TRANSFER Probing with Light More Light Diffraction Diffusion Dynamic Light Scattering NMR Self-Diffusion Coefficient *Each chapter provides an Introduction, General Topic and Text References, and Problems. Worked examples also appear throughout the chapters.