gebhart/patrepeval huggingface dataset:
Hemodynamics Is a Key Epigenetic Factor in Development of the Cardiac Conduction System (2003)

The His-Purkinje system (HPS) is a network of conduction cells responsible for coordinating the contraction of the ventricles. Earlier studies using bipolar electrodes indicated that the functional maturation of the HPS in the chick embryo is marked by a topological shift in the sequence of activation of the ventricle. Namely, at around the completion of septation, an immature base-to-apex sequence of ventricular activation was reported to convert to the apex-to-base pattern characteristic of the mature heart. Previously, we have proposed that hemodynamics and/or mechanical conditioning may be key epigenetic factors in development of the HPS. We thus hypothesized that the timing of the topological shift marking maturation of the conduction system is sensitive to variation in hemodynamic load. Spatiotemporal patterns of ventricular activation (as revealed by high-speed imaging of fluorescent voltage-sensitive dye) were mapped in chick hearts over normal development, and following procedures previously characterized as causing increased (conotruncal banding, CTB) or reduced (left atrial ligation, LAL) hemodynamic loading of the embryonic heart. The results revealed that the timing of the shift to mature activation displays striking plasticity. CTB led to precocious emergence of mature HPS function relative to controls whereas LAL was associated with delayed conversion to apical initiation. The results from our study indicate a critical role for biophysical factors in differentiation of specialized cardiac tissues and provide the basis of a new model for studies of the molecular mechanisms involved in induction and patterning of the HPS in vivo.
Classless IN-ADDR.ARPA delegation (1998)

This document describes a way to do IN-ADDR.ARPA delegation on nonoctet boundaries for address spaces covering fewer than 256 addresses. The proposed method should thus remove one of the objections to subnet on non-octet boundaries but perhaps more significantly, make it possible to assign IP address space in smaller chunks than 24-bit prefixes, without losing the ability to delegate authority for the corresponding IN-ADDR.ARPA mappings. The proposed method is fully compatible with the original DNS lookup mechanisms specified in [1], i.e. there is no need to modify the lookup algorithm used, and there should be no need to modify any software which does DNS lookups.
Class, Race, and the Discourse of “College for All.” A Response to “Schooling for Democracy” (2011)

We critique the “college for all” discourse by unveiling its relationship to the politics of education, the broader economic and political contexts, and the class and race structures embedded in society and schooling, including higher education. We analyze the current and future labor markets to demonstrate the ways that the “college for all” discourse overstates the need for math and science knowledge and skills within the workforce, and we analyze the debt burdens associated with college attendance and completion to demonstrate that the promised benefits of “college for all” are often illusory for low-income, racially, culturally, and linguistically diverse students. Thus, we argue that “college for all”—just like “no child left behind” and the “race to the top”—functions as an ideological velvet to soften education policy talk, talk that actually carries big sticks that punish the very students proclaimed to be the beneficiaries of the proposed changes in schooling. The results of schooling practices articulated by the “college for all” discourse are (a) the reinforcement of material barriers to the stated aims of educational access and equity, and (b) the fortification of the class and race status quo. We examine the ways that the transformation of schooling must be linked to the establishment of just social, economic, and political institutions, and to the formation of a citizenry prepared to engage in the struggles for these institutions.
Effects of Grape Seed-derived Polyphenols on Amyloid β-Protein Self-assembly and Cytotoxicity* (2008)

Epidemiological evidence suggests that moderate consumption of red wine reduces the incidence of Alzheimer disease (AD). To study the protective effects of red wine, experiments recently were executed in the Tg2576 mouse model of AD. These studies showed that a commercially available grape seed polyphenolic extract, MegaNatural-AZ (MN), significantly attenuated AD-type cognitive deterioration and reduced cerebral amyloid deposition (Wang, J., Ho, L., Zhao, W., Ono, K., Rosensweig, C., Chen, L., Humala, N., Teplow, D. B., and Pasinetti, G. M. (2008) J. Neurosci. 28, 6388-6392). To elucidate the mechanistic bases for these observations, here we used CD spectroscopy, photo-induced cross-linking of unmodified proteins, thioflavin T fluorescence, size exclusion chromatography, and electron microscopy to examine the effects of MN on the assembly of the two predominant disease-related amyloid beta-protein alloforms, Abeta40 and Abeta42. We also examined the effects of MN on Abeta-induced cytotoxicity by assaying 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide metabolism and lactate dehydrogenase activity in Abeta-treated, differentiated pheochromocytoma (PC12) cells. Initial studies revealed that MN blocked Abeta fibril formation. Subsequent evaluation of the assembly stage specificity of the effect showed that MN was able to inhibit protofibril formation, pre-protofibrillar oligomerization, and initial coil --> alpha-helix/beta-sheet secondary structure transitions. Importantly, MN had protective effects in assays of cytotoxicity in which MN was mixed with Abeta prior to peptide assembly or following assembly and just prior to peptide addition to cells. These data suggest that MN is worthy of consideration as a therapeutic agent for AD.
2D-to-3D conversion by using visual attention analysis (2010)

This paper proposes a novel 2D-to-3D conversion system based on visual attention analysis. The system was able to generate stereoscopic video from monocular video in a robust manner with no human intervention. According to our experiment, visual attention information can be used to provide rich 3D experience even when depth cues from monocular view are not enough. Using the algorithm introduced in the paper, 3D display users can watch 2D media in 3D. In addition, the algorithm can be embedded into 3D displays in order to deliver better viewing experience with more immersive feeling. Using visual attention information to give a 3D effect is first tried in this research as far as we know.
Prescription omega-3 fatty acids for the treatment of hypertriglyceridemia (2007)

P-O3FA has demonstrated an efficacy and safety in adult patients with high and very high triglycerides adjunct to diet, and the reduction in serum triglyceride levels was dependent on the baseline triglyceride levels. A large controlled clinical trial is necessary to determine if P-O3FA can be used to reduce CHD risk, either as combined with hydroxymethylglutaryl-coenzyme A reductase inhibitors or as monotherapy.
Off-the-shelf virus specific T-cells for therapy of adenovirus disease in immunosuppressed patients. (2019)

7008 Background: Adenovirus infection can cause significant morbidity and mortality in immunosuppressed patients. Cidofovir is commonly used, but its nephrotoxicity is concerning and efficacy limited. Another approach is to restore the anti-adenovirus immunity. Indeed, virus specific T-cells have been shown to be safe and effective in stem cell transplant recipients. Methods: Immunosuppressed pts with either adenovirus viremia or adenovirus-related end organ damage were enrolled. Most closely HLA-matched adenovirus cytotoxic T lymphocytes (CTLs) were generated by expanding donor derived T-cells with a peptide library derived from the hexon protein of adenovirus serotype 3 in the presence of IL-2 20 IU/ml, IL7 10 ng/ml, IL4 10 ng/ml. After receiving 2x10 5 /kg T cells, pts were monitored for response and adverse events. Results: Eight pts received adenovirus CTLs with one infusion. The Table summarizes their characteristics and responses. Seven pts had complete resolution of their symptoms (CR) and adenovirus becoming undetectable (ND). Those pts are alive to date. The remaining patient initially responded but then lost the response when started on high dose prednisolone for treatment of GVHD to which she eventually succumbed. Best response was achieved after a median time of 13 days [10-36]. No cytokine release syndrome occurred and we did not observe any side effect attributable to the CTLs. Conclusions: The use of off-the-shelf adenovirus CTLs is a feasible, safe, and effective approach to treat severe adenovirus infections in immunosuppressed pts. Clinical trial information: NCT03425526. [Table: see text]
Accuracy of automated DNA sequencing: a multi-laboratory comparison of sequencing results. (1995)

A double-stranded (ds)DNA template of "unknown" sequence was distributed to approximately 80 core DNA sequencing laboratories by the Association of Biomolecular Resource Facilities (ABRF) for automated DNA sequence analysis. Forty-four different facilities responded with 83 usable sequence submissions. These sequences were grouped by both sequencing protocol (dye-primer or dye-terminator) and whether manually edited or not. The sequences were aligned with the known sequence, and the number of correct base calls, insertions, deletions, no-calls and miscalls were determined for each group. The dye-primer sequencing protocol provided the longest and most accurate sequence. The edited dye-primer data were > 95% accurate out to 400-450 bp, while the edited dye-terminator data could call only 300-350 bases at this accuracy. However, 75% of the laboratories in this sampling preferred the dye-terminator protocol, presumably because of its versatility and convenience. Laboratories that manually edited the automatically called data were able to obtain an additional 100 bases of good sequence when the dye-primer protocol was used. Surprisingly though, editing of dye-terminator results did not increase the amount of good sequence, although the dye-terminator protocol had a superior base-calling ability within the first 100 bases of called sequence.
E47 Controls the Developmental Integrity and Cell Cycle Quiescence of Multipotential Hematopoietic Progenitors (2008)

Little is known about the transcriptional regulators that control the proliferation of multipotent bone marrow progenitors. Understanding the mechanisms that restrict proliferation is of significant interest since the loss of cell cycle integrity can be associated with hematopoietic exhaustion, bone marrow failure, or even oncogenic transformation. Herein, we show that multipotent LSKs (lineage(-)Sca(high)c-kit(+)) from E47-deficient mice exhibit a striking hyperproliferation associated with a loss of cell cycle quiescence and increased susceptibility to in vivo challenge with a mitotoxic drug. Total LSKs contain long-term self-renewing hematopoietic stem cells and downstream multipotential progenitors (MPPs) that possess very limited or no self-renewal ability. Within total LSKs, we found specific developmental and functional deficits in the MPP subset. E47 knockout mice have grossly normal numbers of self-renewing hematopoietic stem cells but a 50-70% reduction in nonrenewing MPPs and downstream lineage-restricted populations. The residual MPPs in E47 knockout mice fail to fully up-regulate flk2 or initiate V(D)J recombination, hallmarks of normal lymphoid lineage progression. Consistent with the loss of normal cell cycle restraints, we show that E47-deficient LSKs have a 50% decrease in p21, a cell cycle inhibitor and known regulator of LSK proliferation. Moreover, enforced expression studies identify p21 as an E47 target gene in primary bone marrow LSKs. Thus, E47 appears to regulate the developmental and functional integrity of early hematopoietic subsets in part through effects on p21-mediated cell cycle quiescence.
Muscle-specific expression of the cardiac alpha-actin gene requires MyoD1, CArG-box binding factor, and Sp1. (1990)

Expression of the human cardiac alpha-actin gene (HCA) depends on the interactions of multiple transcriptional regulators with promoter elements. We report here that the tissue-specific expression of this promoter is determined by the simultaneous interaction of at least three specific protein-DNA complexes. The myogenic determinant gene MyoD1 activated the transcription of transfected HCA-CAT promoter constructs in nonmuscle cells, including CV-1 and HeLa cells. Gel mobility-shift and footprinting assays revealed that MyoD1 specifically interacted with a single consensus core sequence, CANNTG, at -50. Previously characterized sites interact with a protein identical with or related to the serum response factor (SRF) at -100 and Sp1 at -70. All three elements must be intact to support transcription in muscle cells: site-specific mutation within any one of these three elements eliminated transcriptional expression by the promoter. Furthermore, expression of the promoter in embryonic Drosophila melanogaster cells that lack MyoD1 and Sp1 is strictly dependent on all three sites remaining intact and on the presence of exogenously supplied Sp1 and MyoD1. These experiments suggest that the presence of three sequence-specific binding proteins, including MyoD1, and their intact target DNA sequences are minimal requirements for muscle-specific expression of the HCA gene.
Molecular and biological characterization of anticarcinogenic and tumor cell growth-inhibitory activities of Syrian hamster lymphotoxin. (1983)

Syrian hamster lymphotoxin has three identified anticancer activities: (a) cytolytic activity measured as the release of 3H from [3H]thymidine-labeled murine alpha L-929 cells; (b) cytostatic activity assessed by the inhibition of cell colony formation of tumorigenic Syrian hamster cells; and (c) anticarcinogenic activity measured by inhibition of chemical carcinogen or radiation-induced morphological transformation of Syrian hamster fetal cells in vitro and in vivo. Lymphotoxin cytolytic, cytostatic, and anticarcinogenic activities have similar molecular weights of approximately 45,000 by ACA-44 Ultrogel chromatography. A three-step purification of (a) diafiltration and concentration, (b) column isoelectric focusing in a pH 4 to 6 gradient, and (c) ACA-44 Ultrogel column chromatography yields 25% recovery of lymphotoxin cytolytic and anticarcinogenic activities. The three-step purified lymphotoxin is free of detectable interferon, macrophage migration inhibitory factor, and mitogenic factors inducing hamster T-lymphocyte proliferation and is anticarcinogenic in vivo. The purified lymphotoxin had a specific anticarcinogenic activity of 58,500 units/mg of protein and a molar specific activity of 2,900 units/nmol. Although homogeneous in molecular size, the purified lymphotoxin is comprised of several molecular species. Cytolytic activity is associated with molecules having a single major isoelectric point of 5.0, whereas the cytostatic and anticarcinogenic activities comigrate with molecules with major isoelectric points of 5.0 and 4.6. This is the first evidence that the anticancer actions of lymphotoxin are properties of similarly sized but several different electrically charged molecules and that the several lymphotoxin species have differing anticancer activities.
Sugar‐Selective Enrichment of a D‐Glucose‐Substituted Ruthenium Bipyridyl Complex Inside HepG2 Cancer Cells (2010)

Ru sugar balls: RuII bipyridyl complexes bearing different sugar units were synthesized. Incubation of these compounds with HepG2 cells shows for the first time that cellular uptake of equally substituted ruthenium bipyridyl complexes can be controlled by the type of sugar attached. Preferential internalization of the D-glucose-bearing complex was observed and verified by confocal laser scanning microscopy (CLSM) and flow cytometry.
Left-right axis malformations associated with mutations inACVR2B, the gene for human activin receptor type IIB (1999)

Targeted disruption of the mouse activin receptor type IIB gene (Acvr2b) results in abnormal left-right (LR) axis development among Acvr2b-/- homozygotes [Oh and Li, 1997: Genes Dev 11:1812-1826]. The resulting malformations include atrial and ventricular septal defects, right-sided morphology of the left atrium and left lung, and spleen hypoplasia. Based on these results, we hypothesized that mutations in the type IIB activin receptor gene are associated with some cases of LR axis malformations in humans. We report here characterization of the ACVR2B genomic structure, analysis of ACVR2B splice variants, and screening for ACVR2B mutations among 112 sporadic and 14 familial cases of LR axis malformations. Two missense substitutions have been identified, one of which appears in two unrelated individuals. Neither of these nucleotide changes has been found in 200 control chromosomes. We conclude that ACVR2B mutations are present only rarely among human LR axis malformation cases.
CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca 2+ Entry (2006)

Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery.
Photolysis of polymeric organosilicon systems. 4. Photochemical behavior of poly[p-(disilanylene)phenylene] (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotolysis of polymeric organosilicon systems. 4. Photochemical behavior of poly[p-(disilanylene)phenylene]Kazuo. Nate, Mitsuo. Ishikawa, Hongzhi. Ni, Hiroyuki. Watanabe, and Yukitami. SahekiCite this: Organometallics 1987, 6, 8, 1673–1679Publication Date (Print):August 1, 1987Publication History Published online1 May 2002Published inissue 1 August 1987https://pubs.acs.org/doi/10.1021/om00151a011https://doi.org/10.1021/om00151a011research-articleACS PublicationsRequest reuse permissionsArticle Views161Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Large-Area Synthesis of Highly Crystalline WSe2 Monolayers and Device Applications (2013)

The monolayer transition metal dichalcogenides have recently attracted much attention owing to their potential in valleytronics, flexible and low-power electronics, and optoelectronic devices. Recent reports have demonstrated the growth of large-size two-dimensional MoS2 layers by the sulfurization of molybdenum oxides. However, the growth of a transition metal selenide monolayer has still been a challenge. Here we report that the introduction of hydrogen in the reaction chamber helps to activate the selenization of WO3, where large-size WSe2 monolayer flakes or thin films can be successfully grown. The top-gated field-effect transistors based on WSe2 monolayers using ionic gels as the dielectrics exhibit ambipolar characteristics, where the hole and electron mobility values are up to 90 and 7 cm(2)/Vs, respectively. These films can be transferred onto arbitrary substrates, which may inspire research efforts to explore their properties and applications. The resistor-loaded inverter based on a WSe2 film, with a gain of ∼13, further demonstrates its applicability for logic-circuit integrations.
The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress—elucidation by GADD34‐deficient mice (2003)

GADD34 is a protein that is induced by stresses such as DNA damage. The function of mammalian GADD34 has been proposed by in vitro transfection, but its function in vivo has not yet been elucidated. Here we generated and analyzed GADD34 knockout mice. Despite their embryonic stage- and tissue-specific expressions, GADD34 knockout mice showed no abnormalities at fetal development and in early adult life. However, in GADD34-/- mouse embryonic fibroblasts (MEFs), recovery from a shutoff of protein synthesis was delayed when MEFs were exposed to endoplasmic reticulum (ER) stress. The phosphorylation of eukaryotic translation initiation factor 2 alpha (eIF2alpha) at Ser51 induced by thapsigargin or DTT was prolonged in GADD34-/- MEF, although following treatment with tunicamycin, the eIF2alpha phosphorylation level did not change in either GADD34+/+ or GADD34-/- cells. ER stress stimuli induced expressions of Bip (binding Ig protein) and CHOP (C/EBP homologous protein) in MEF of wild-type mice. These expressions were strongly reduced in GADD34-/- MEF, which suggests that GADD34 up-regulates Bip and CHOP. These results indicate that GADD34 works as a sensor of ER stress stimuli and recovers cells from shutoff of protein synthesis.
Mode of Action of Vibriocin (1970)

The mechanism of action of vibriocin, a bacteriocin produced by Vibrio comma, was investigated. Its lethal action (as defined by the loss in colony-forming ability) was reversed by tryptic digestion within 7 to 10 min after adsorption. The bacteriocin had a pronounced inhibitory effect on deoxyribonucleic acid (DNA) synthesis, whereas ribonucleic acid (RNA) and protein synthesis continued, although at a reduced rate. Chloramphenicol protected sensitive bacteria from the lethal action. Degradation of bacterial DNA prelabeled with (3)H-thymidine, as measured by changes in acid-precipitable radioactivity, occurred 10 min after treatment with vibriocin. The bacteriocin per se had no detectable deoxyribonuclease activity. Observation of vibriocin-treated cells by phase-contrast microscopy, measurement of ultraviolet light-absorbing capacity of extracellular fluid, and (42)K-efflux studies indicated a damaged bacterial membrane. This impairment of membrane function occurred in the presence of chloramphenicol and thus, unlike the lethal effect of vibriocin, was independent of protein synthesis.
Erratum to: Lack of functional and expression homology between human and mouse aldo-keto reductase 1C enzymes: implications for modelling human cancers (2010)

It has come to our attention that since publication of our article The experiments in this study used androstanedione as a test substrate. Androstenedione was not used in any experiments. This error does not in any way invalidate the conclusion of the paper that aldo-keto reductases of the mouse and human 1C-subfamilies lack functional and expression homology. The authors apologise for any confusion caused.