gebhart/patrepeval huggingface dataset:
36B4 cDNA used as an estradiol-independent mRNA control is the cDNA for human acidic ribosomal phosphoprotein PO (1991)

36B4 is a human cDNA clone originally isolated by the group of P.Chambon (1) in 1982, from a differential cDNA library of estradiol stimulated versus non stimulated MCF-7 breast cancer cells. Because 36B4 mRNA levels are not modified by estradiol treatment, this cDNA probe is widely used as a control in RNAse protection experiments to study the regulation by estradiol of the transcription levels of several genes. So far, the identity of 36B4 was unknown. In an attempt to identify 36B4, partial sequencing of a Pstl-PstI cDNA fragment of 36B4 subcloned in the plasmid pGEM 4Z (Promega, Madison, WI) was performed by the method of Sanger et al. (2), and the T7 promoter oligonucleotide (Promega) as a primer. Homology search of the Genbank and EMBL sequence databases was performed by using the program FASTA, created by Lipman and Pearson (3), accessed through the University of Wisconsin Genetic Computer Group (UWGCG) software package. Figure The 158 bp fragment of 36B4 shows also 88.5% homology with the mouse PO cDNA (5) (Genbank accession number XI5267) and 87.8% with the rat PO cDNA (6) (Genbank accession number XI5096). Those facts, taken together, leave little doubt about the identity of 36B4. This identity can give some insight on why the 36B4 mRNA levels are unmodified by estradiol, given the fact that the PO protein levels seem to be under translational control (5). Since mouse and rat PO cDNAs are also cloned, researchers may now obtain riboprobes from those cDNAs to be used as identical controls in studies of transcriptional regulation in these species.
Effect of interferon on GB virus C and hepatitis C virus in hepatitis patients with the co-infection (1997)

Of 74 patients who were infected with hepatitis C virus (HCV) and received interferon, 12 (16%) were positive for RNA of GB virus C (GBV-C). RNA of GBV-C was determined in sera from the co-infected patients retrospectively, and the effect of interferon on GBV-C was compared with that on HCV in them. Titers of both GBV-C and HCV RNAs decreased during interferon in all of them. Two patients lost both GBV-C and HCV RNAs and remained clear until 6 months after treatment with interferon, while 2 lost RNA for GBV-C only and 2 for HCV RNA alone. Low pretreatment RNA titers of GBV-C and HCV correlated with the efficacy of interferon in clearing. Alanine aminotransferase returned to normal only in the patients who lost HCV RNA, regardless of the persistence or loss of GBV-C RNA. These results indicate that the response to interferon of GBV-C is comparable to but independent of that of HCV and that the persistence of GBV-C would not prevent the normalization of aminotransferases in response to interferon in patients with chronic hepatitis C.
Estimating a Signal from a Magnitude Spectrogram via Convex Optimization (2012)

The problem of recovering a signal from the magnitude of its short-time Fourier transform (STFT) is a longstanding one in audio signal processing. Existing approaches rely on heuristics that often perform poorly because of the nonconvexity of the problem. We introduce a formulation of the problem that lends itself to a tractable convex program. We observe that our method yields better reconstructions than the standard Griffin-Lim algorithm. We provide an algorithm and discuss practical implementation details, including how the method can be scaled up to larger examples.
International Escalation and the Dollar Auction (1986)

Two players bid for a dollar on the condition that both the loser and the winner must pay the bids, although only the winner will receive the dollar. Collusion or threats are excluded; bids must be in units of nickels; and a player does not bid in a situation in which bidding and not bidding would lead to the same payoff. We derive a formula for the players' rational strategies, which implies, for example, that when both have $2.50 available, the first bidder should open by bidding $.60 and the other should remain silent. The fact that rational players would not bid against each other shows that unlike the Prisoners' Dilemma, the dollar auction is not innately a trap, but exploits some irrationality in the bidders' behavior. The dollar auction resembles international escalation in that the governments in conflict commit resources that will not be returned. Some points of difference in the two situations are listed and discussed.
面向快速增长的光电子市场IC China 2005将设光电子及平板显示展区 (2005)

光电子及平板显示技术是当今科技热点，也是电子信息产业发展的重点领域之一，近几年国内光电子及平板显示市场呈现出正蓬勃发展的势头。根据赛迪顾问的数据，2004年中国发光二极管(LED)市场销售额为115．1亿元，比2003年同比大幅增长27．5％，手机、显示屏、指示灯、LCD背光源、照明以及装饰灯等领域对LED产品的需求都有大幅增长。目前中国已经占据全球LED市场四分之一的份额。未来在国家半导体工程启动的带动下，国内LED市场将以年均近20％的速度增长，预计到2009年其市场规模将达到282亿元，在全球市场中所占份额件上升到三分之一强。
Complete cDNA sequence coding for the human T cell receptor a chain of HFB-ALL (1988)

Journal Article Complete cDNA sequence coding for the human T cell receptor a chain of HFB-ALL Get access Ben Berkhout, Ben Berkhout * Laboratory of Molecular Immunology, Dana-Farber Cancer Institute44 Binney Street, Boston, MA 02115, USA * Holder of a Dutch Cancer Society (KWF) Fellowship, and addressee for all correspondence Search for other works by this author on: Oxford Academic PubMed Google Scholar Craig Hall, Craig Hall Laboratory of Molecular Immunology, Dana-Farber Cancer Institute44 Binney Street, Boston, MA 02115, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Cox Terhorst Cox Terhorst Laboratory of Molecular Immunology, Dana-Farber Cancer Institute44 Binney Street, Boston, MA 02115, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 11, 10 June 1988, Page 5209, https://doi.org/10.1093/nar/16.11.5209 Published: 10 June 1988 Article history Accepted: 09 March 1988 Published: 10 June 1988
Autocrine Growth Factor Production by Fetal, Keloid, and Normal Dermal Fibroblasts (2003)

Both fetal and keloid fibroblasts produce significantly more TGF-beta1 than normal adult fibroblasts. Our data and the data of others suggest that fetal fibroblasts produce more bFGF than adult fibroblasts. The serum-free model we describe can be used to quantitatively measure autocrine growth factor production by cells that underlie clinically different types of wound healing. This model provides information that may allow us to better treat and prevent undesirable scarring.
Coherent Two-Photon Excitation by Multiple Light Pulses (1977)

We have studied Doppler-free two-photon excitation of atoms with a train of phase-coherent standing-wave light pulses, originating from the same laser pulse. Quantum interference effects produce narrow spectral fringes which have a physical origin similar to that of Ramsey fringes. Linewidths much less than the Fourier-transform limit of an individual light pulse have been observed for the sodium $3S\ensuremath{-}5S$ transition; and a dramatic enhancement of the resonant signal is possible.
Viral and Non-Viral Methods to Genetically Modify Dendritic Cells (2012)

Dendritic cells (DCs) behave as antigenic or tolerogenic immune response inducers depending on the nature of their precursors, their differentiation pathway and their environment. As professional antigen presenting cells (APCs) it has been tempting to genetically modify them in order to improve their capacity to mount appropriate protective immune responses. Gene transfer may also be helpful to investigate fundamental issues about the DC biology. Of note, almost all strategies to deliver genes or interfering RNA into DCs have been used with different success rates. These methods are non-exhaustively presented and discussed here. We focused our attention on promising in vitro as well as in vivo lentiviral- mediated gene delivery solutions into murine or human DCs.
Definition, epidemiology and magnitude of alcoholic hepatitis (2011)

Alcoholic liver disease (ALD) is a major cause of alcohol-related morbidity and mortality. Its presentation ranges from fatty liver to alcoholic hepatitis (AH), cirrhosis, and hepatocellular carcinoma. Although the amount and pattern of alcohol consumption is a well recognized predisposing factor for the development of serious liver pathology, environmental factors and the host's genetic make-up may also play significant roles that have not yet been entirely explored. Continuing alcohol consumption is a major factor that influences the survival of patients with AH. The presence of cirrhosis at presentation or its development on follow up is a major factor determining the outcome in the long run. This chapter deals with the epidemiology and magnitude of ALD in general and AH in particular.
Quantum size effects in nanocrystalline semiconducting titania layers prepared by anodic oxidative hydrolysis of titanium trichloride (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantum size effects in nanocrystalline semiconducting titania layers prepared by anodic oxidative hydrolysis of titanium trichlorideLadislav Kavan, Tiziana Stoto, Michael Graetzel, Donald Fitzmaurice, and Valery ShkloverCite this: J. Phys. Chem. 1993, 97, 37, 9493–9498Publication Date (Print):September 1, 1993Publication History Published online1 May 2002Published inissue 1 September 1993https://pubs.acs.org/doi/10.1021/j100139a038https://doi.org/10.1021/j100139a038research-articleACS PublicationsRequest reuse permissionsArticle Views972Altmetric-Citations158LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Tumor necrosis factor-alpha and soluble tumor necrosis factor receptors in the culture supernatants of polymorphonuclear cells and peripheral blood mononuclear cells from cancer patients. (1998)

Recent clinical and experimental studies have focused on the measurement of cytokines and their regulators, produced by immunocompetent cells. Their estimation may be used as parameters for the immune potential of cancer patients. In the present study we studied the ability of unstimulated and lipopolysaccharide (LPS)-stimulated polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC) from oral cavity cancer and breast cancer patients to release tumor necrosis factor alpha (TNF-alpha) and soluble tumor necrosis factor receptors (sTNFR). There were significant differences concerning the parameters examined for PMN and PBMC from cancer patients as compared with normal subjects. We found significantly higher concentrations of sTNF-R p75 than sTNF-R p55 in the cell-culture supernatants. The culture supernatants of cells from oral cavity cancer patients contained higher concentrations of TNF-alpha and lower concentrations of sTNF-R p55 and sTNF-R p75 in comparison with breast cancer cell supernatants. In contrast, cells from breast cancer patients secreted lower concentrations of TNF-alpha and higher concentrations of sTNF-R p55 and sTNF-R p75. Although PBMC secreted higher concentrations of mediators than PMN, the quantitative dominance of PMN in the peripheral blood suggests an essential role of these cells in the defense reactions controlled by TNF-alpha.
Integrated Capacitance Resistive Model for Reservoir Characterization in Primary and Secondary Recovery (2011)

Abstract This paper presents a new procedure to obtain unique estimates of interwell connectivities, pore volume, and productivity index in primary and secondary recovery. This method approaches the problem by analytically integrating the simplified continuity material balance equation. The proposed procedure uses linear multivariate regression on rate, initial reservoir pressure and bottom hole pressure (BHP) data. The method quantifies compressible pore volume – the capacitance, productivity index (PI), and degree of communication between well pairs – the resistance to flow. Hence, it is called the integrated capacitance-resistive (ICR) model. The ICR model fits cumulative production against rates, BHP, and cumulative water injected. For primary recovery, the model has been validated on synthetic field data generated by numerical simulator, covering the effects of initial oil saturation, heterogeneity, and number of wells. The model was also tested on a real field. For secondary recovery, the procedure was tested on synthetic fields with high permeability channels. In all primary recovery synthetic fields tested, good fit and less than 13% error of estimated pore volume and PI were observed. Real field application also shows good agreement with geological knowledge. For waterflooded synthetic fields, the estimated parameters are consistent with the geology and the results obtained from the capacitance resistive model (CRM) fit. The confidence intervals of the parameters are narrow enough to conclude regression coefficients are statistically significant. Hence, the ICR model can be used to quickly estimate reservoir properties and infer interwell communication in primary and secondary recovery from available data with high confidence. The ICR model is comparable to CRM in terms of number of inputs. Hence, both models can be used in large fields with hundreds of wells. However, the nature of the CRM's nonlinearity makes it difficult to establish confidence intervals of the model parameters and obtain a unique set of model parameters for a large number of wells. In the proposed method, a unique solution can always be obtained regardless of the number of parameters via linear regression. The simpler formulation of the ICR model would reduce the computation time and easily establish the confidence intervals of model parameters.
A Single, Abbreviated RPGR-ORF15 Variant Reconstitutes RPGR Function In Vivo (2005)

RPGR-ORF15 is the functionally significant variant in photoreceptors. The length of its repetitive region can be reduced while preserving its function. The current findings should facilitate the design of gene replacement therapy for RPGR-null mutations.
Tubular Shear Stress and Phenotype of Renal Proximal Tubular Cells (2003)

Phenotypic alterations resulting from flow-induced mechanical strains is a growing field of research in many cell types such as vascular endothelial and smooth muscle cells, chondrocytes, and osteocytes. Because renal mass reduction is followed by a dramatic increase in GFR in the remaining nephron, modulation of tubular cell phenotype by flow-induced mechanical strains could be one of the events initiating the deleterious pathways that lead to the destruction of renal parenchyma after renal mass reduction. This study demonstrates that increased flow induced, in vitro and in vivo, a reinforcement of the apical domain of actin cytoskeleton and an inhibition of plasminogen activator expression. These effects of flow on plasminogen activator expression were prevented by blocking the reorganization of actin cytoskeleton and were associated with an increase in a shear-stress responsive element binding activity. These results confirm that tubular flow affects the phenotype of renal epithelial cells and suggest that flow-induced mechanical strains could be one determinant of tubulointerstitial lesions during the progression of renal diseases.
Cloning, Expression, Sequence Analysis, and Characterization of Streptokinases Secreted by Porcine and Equine Isolates of Streptococcus equisimilis (1999)

Streptokinases secreted by nonhuman isolates of group C streptococci (Streptococcus equi, S. equisimilis, and S. zooepidemicus) have been shown to bind to different mammalian plasminogens but exhibit preferential plasminogen activity. The streptokinase genes from S. equisimilis strains which activated either equine or porcine plasminogen were cloned, sequenced, and expressed in Escherichia coli. The streptokinase secreted by the equine isolate had little similarity to any known streptokinases secreted by either human or porcine isolates. The streptokinase secreted by the porcine isolate had limited structural and functional similarities to streptokinases secreted by human isolates. Plasminogen activation studies with immobilized (His)(6)-tagged recombinant streptokinases indicated that these recombinant streptokinases interacted with plasminogen in a manner similar to that observed when streptokinase and plasminogen interact in the fluid phase. Analysis of the cleavage products of the streptokinase-plasminogen interaction indicated that human, equine, and porcine plasminogens were all cleaved at the same highly conserved site. The site at which streptokinase was cleaved to form altered streptokinase (Sk*) was also determined. This study confirmed not only the presence of streptokinases in nonhuman S. equisimilis isolates but also that these proteins belong to a family of plasminogen activators more diverse than previously thought.
Comparative In Vitro Evaluation of Two Different Preparations of Small Diameter Polyurethane Vascular Grafts (2000)

Polyurethane (PU) frequently has been used to manufacture small diameter vascular grafts due to its good bicompatibility. In this study, sponge PU small diameter vascular grafts were fabricated from Pellethane 2103, as well as a self-synthesized PU, by utilization of a salt casting technique and by varying the salt/polymer ratios. Two types were made; one of them had a thin solid layer on the outer surface. The inner surface was identical and was coated with gelatin crosslinked by epoxide. Tensile properties, compliance, platelet activation, and endothelial cell attachment were evaluated in vitro. It was found that for the grafts with an outer nonporous coating, compliance could not be estimated from modulus due to anisotropy. The most compliance-matched grafts were made of self-synthesized polyurethane with a salt/polymer ratio 4 to 8 or Pellethane with a ratio 6 to 8 without the outer nonporous coating. The former had better elongation. Self-synthesized PU had lower platelet adhesion as well as more endothelial attachment than Pellethane. Porosity activated platelets strongly and reduced endothelial adhesion unless the surface was modified by the crosslinked gelatin layer. It was concluded that PU synthesized in our lab with a salt/polymer ratio of at least 4 and coated with epoxy crosslinked gelatin was a better substrate for preparation of small diameter vascular grafts.
Fcγ Receptor–mediated Induction of Dendritic Cell Maturation and Major Histocompatibility Complex Class I–restricted Antigen Presentation after Immune Complex Internalization (1999)

Dendritic cells (DCs) express several receptors for the Fc portion of immunoglobulin (Ig)G (FcgammaR), which mediate internalization of antigen-IgG complexes (immune complexes, ICs) and promote efficient major histocompatibility complex (MHC) class II-restricted antigen presentation. We now show that FcgammaRs have two additional specific attributes in murine DCs: the induction of DC maturation and the promotion of efficient MHC class I-restricted presentation of peptides from exogenous, IgG-complexed antigens. Both FcgammaR functions require the FcgammaR-associated gamma chain. FcgammaR-mediated MHC class I-restricted antigen presentation is extremely sensitive and specific to immature DCs. It requires proteasomal degradation and is dependent on functional peptide transporter associated with antigen processing, TAP1-TAP2. By promoting DC maturation and presentation on both MHC class I and II molecules, ICs should efficiently sensitize DCs for priming of both CD4(+) helper and CD8(+) cytotoxic T lymphocytes in vivo.
Metal-Organic Framework Templated Synthesis of Ultrasmall Catalyst Loaded ZnO/ZnCo2O4 Hollow Spheres for Enhanced Gas Sensing Properties (2017)

To achieve the rational design of nanostructures for superior gas sensors, the ultrasmall nanoparticles (NPs) loaded on ternary metal oxide (TMO) hollow spheres (HS) were synthesized by using the polystyrene (PS) sphere template and bimetallic metal-organic framework (BM-MOFs) mold. The zinc and cobalt based zeolite imidazole frameworks (BM-ZIFs) encapsulating ultrasmall Pd NPs (2-3 nm) were assembled on PS spheres at room temperature. After calcination at 450 °C, these nanoscale Pd particles were effectively infiltrated on the surface of ZnO/ZnCo<sub>2</sub>O<sub>4</sub> HSs. In addition, the heterojunctions of Pd-ZnO, Pd-ZnCo<sub>2</sub>O<sub>4</sub>, and ZnO-ZnCo<sub>2</sub>O<sub>4</sub> were formed on each phase. The synthesized Pd-ZnO/ZnCo<sub>2</sub>O<sub>4</sub> HSs exhibited extremely high selectivity toward acetone gas with notable sensitivity (S = 69% to 5 ppm at 250 °C). The results demonstrate that MOF driven ultrasmall catalyst loaded TMO HSs were highly effective platform for high performance chemical gas sensors.
ESOP-1, a secreted protein expressed in the hematopoietic, nervous, and reproductive systems of embryonic and adult mice (2000)

To isolate soluble factors expressed in early phases of hematopoietic differentiation, we applied the signal sequence trap method to the in vitro murine hematopoietic differentiation system, in which ES cells are cocultured with OP-9 stroma cells. This strategy allowed us to isolate cDNA for a secreted protein, ESOP-1, of 160 amino acids, the sequence of which shows 64% identity with human ESOP-1/MD-2. ESOP-1 mRNA was highly expressed in the mouse embryos at 7.5 days after coitus. Expression of the ESOP-1 mRNA and protein was shown in the embryonic and adult hematopoietic system. In addition, the ESOP-1 protein was found in the yolk sac-blood islands, the developing nervous system, and the adult reproductive system. These results suggest that ESOP-1 may play some roles in the development or maintenance of hematopoietic, nervous, and reproductive systems.