gebhart/patrepeval huggingface dataset:
Interleukins 4 and 13 Increase Intestinal Epithelial Permeability by a Phosphatidylinositol 3-Kinase Pathway (2000)

Interleukins 4 and 13 can affect their target cells by activation of signal transducer and activator of transcription 6 (STAT 6) or phosphatidylinositol 3-kinase (PI3K). We examined the signal transduction events involved in IL-4 and IL-13 regulation of epithelial paracellular permeability using T84 cells, a model human colonic epithelium. T84 cells treated with IL-4 or IL-13 displayed virtually identical dose- and time-dependent STAT 6 activation as assessed by electrophoretic mobility shift assay (EMSA) and decreases in transepithelial resistance (TER). STAT 6 DNA binding activity was maximal in nuclear extracts 30 min after exposure to IL-4 or IL-13, and TER was maximally reduced by 24 h post-treatment. Pretreatment of epithelia with transcription factor decoys (phosphorothioated DNA oligonucleotides containing the STAT 6 binding site) dramatically reduced STAT 6 activation as detected by EMSA, but did not attenuate the TER reduction by IL-4 or IL-13. In contrast, although the PI3K inhibitors wortmannin and LY294002 did not affect IL-4 or IL-13 STAT 6 activation, they significantly inhibited the ability of either cytokine to lower TER. Thus, we provide evidence for PI3K as the major proximal signaling event in IL-4 and IL-13 regulation of TER and speculate that pharmacological targeting of enterocytic PI3K activity may represent a means to manipulate epithelial permeability.
X-Ray Absorption Fine Structure Study on Layered LiMO[sub 2] (M=Ni, Mn, Co) Cathode Materials (2006)

Systematic investigation of layered cathode materials was performed to elucidate how Co and Li contribute to the electrochemical properties from electronic and structural points of view. Ni, Mn, and Co K-edge X-ray absorption near-edge structure (XANES) study showed that the Ni valence state was controlled not only by Mn content but also by Co and Li contents in ( and ) series. Li has larger influence on the Ni valence than Co does. In extended X-ray absorption fine structure (EXAFS) curve-fitting analysis, shortening of Ni–O bond length was observed as increased (excess Li increased) or decreased (Co increased), which is also attributed to Ni oxidation. In contrast to Ni, Mn and Co valence states and local structures showed no significant differences on changing the metal content. This study revealed that Li contributes to the rate capability through the concentration of in the transition metal layers, which is closely related to the powder volume resistivity of the active material. Co contributes to the Li-ion conductivity through less cation mixing than to the electronic conductivity of the pristine powder.
<title>Visual Model-Based Algorithms For Halftoning Images</title> (1981)

The limitations of human vision provide the justification for design of systems for printing or display of halftone images that are perceived to be nearly identical to a continuous-tone original. Despite this fact, a review of existing halftone algorithms indicates that only limited use has been made of the extensive research on human vision. This review also emphasizes the common features of the many algorithms that have been proposed. It is suggested that iterative techniques may provide a means of incorporating a much more complete visual model within the halftoning algorithm.
Some considerations for a high performance message-based interprocess communication system (1975)

We continue to be concerned with interprocess communications systems (such as those described in references 1, 2, and 3 and called “thin-wire” communications systems in reference 4) which are suitable for communication between processes that are not co-located in the same operating system but rather reside in different operating systems on different computers connected by a computer communications network. Further, the systems with which we are concerned are assumed to communicate using addressed messages (e.g., reference 5) which are multiplexed onto the logical communications channel between the source process and the destination process, rather than using such traditional methods as shared memory (an impossibility for distributed communicating processes) or dedicated physical communications channels between pairs of processes desiring to communicate (which is considered to be prohibitively expensive).
Practical process research and development a guide for organic chemists (2012)

Designed to provide a comprehensive, step-by-step approach to organic process research and development in the pharmaceutical, fine chemical, and agricultural chemical industries, this book describes the steps taken, following synthesis and evaluation, to bring key compounds to market in a cost-effective manner. It describes hands-on, step-by-step, approaches to solving process development problems, including route, reagent, and solvent selection; optimising catalytic reactions; chiral syntheses; and "green chemistry." Second Edition highlights: Reflects the current thinking in chemical p
Transcriptional mapping of human respiratory syncytial virus (1984)

A transcriptional map for human respiratory syncytial virus was determined by measuring the kinetics of viral gene inactivation in response to UV irradiation. Monolayer cell cultures of respiratory syncytial virus-infected HEp-2 cells were exposed to UV light, and residual viral RNA synthesis was monitored both by gel electrophoresis and by hybridization to dot blots of cloned cDNAs of the 10 known viral genes. Target sizes for the 10 individual viral genes were calculated relative to the UV sensitivity of intracellular viral genome replication. Target size analysis indicated that the 10 viral genes were transcribed as a single transcriptional unit and that the transcription of an individual gene was dependent on the prior transcription of all the preceding genes. The order of gene transcription (with nomenclature according to encoded proteins) was determined to proceed from the promoter as follows: 14K, 11K, N, P, M, 9.5K, 36K, F, 24K, L.
Learning Role-based Graph Embeddings (2018)

Random walks are at the heart of many existing network embedding methods. However, such algorithms have many limitations that arise from the use of random walks, e.g., the features resulting from these methods are unable to transfer to new nodes and graphs as they are tied to vertex identity. In this work, we introduce the Role2Vec framework which uses the flexible notion of attributed random walks, and serves as a basis for generalizing existing methods such as DeepWalk, node2vec, and many others that leverage random walks. Our proposed framework enables these methods to be more widely applicable for both transductive and inductive learning as well as for use on graphs with attributes (if available). This is achieved by learning functions that generalize to new nodes and graphs. We show that our proposed framework is effective with an average AUC improvement of 16.55% while requiring on average 853x less space than existing methods on a variety of graphs.
PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration (2016)

Herein, we developed honeycomb-like scaffolds by combining poly (d, l-lactic acid) (PDLLA) with a high amount of graphene/multi-walled carbon nanotube oxides (MWCNTO-GO, 50% w/w). From pristine multi-walled carbon nanotubes (MWCNT) powders, we produced MWCNTO-GO via oxygen plasma etching (OPE), which promoted their exfoliation and oxidation. Initially, we evaluated PDLLA and PDLLA/MWCNTO-GO scaffolds for tensile strength tests, cell adhesion and cell viability (with osteoblast-like MG-63 cells), alkaline phosphatase (ALP, a marker of osteoblast differentiation) activity and mineralized nodule formation. In vivo tests were carried out using PDLLA and PDLLA/MWCNTO-GO scaffolds as fillers for critical defects in the tibia of rats. MWCNTO-GO loading was responsible for decreasing the tensile strength and elongation-at-break of PDLLA scaffolds, although the high mechanical performance observed (~600MPa) assures their application in bone tissue regeneration. In vitro results showed that the scaffolds were not cytotoxic and allowed for osteoblast-like cell interactions and the formation of mineralized matrix nodules. Furthermore, MG-63 cells grown on PDLLA/MWCNTO-GO significantly enhanced osteoblast ALP activity compared to controls (cells alone), while the PDLLA group showed similar ALP activity when compared to controls and PDLLA/MWCNTO-GO. Most impressively, in vivo tests suggested that compared to PDLLA scaffolds, PDLLA/MWCNTO-GO had a superior influence on bone cell activity, promoting greater new bone formation. In summary, the results of this study highlighted that this novel scaffold (MWCNTO-GO, 50% w/w) is a promising alternative for bone tissue regeneration and, thus, should be further studied.
Selective thromboxane synthetase inhibitors and antihypertensive agents. New derivatives of 4-hydrazino-5H-pyridazino[4,5-b]indole, 4-hydrazinotriazino[4,5-a]indole, and related compounds (1987)

A series of new derivatives of 4-hydrazino-5H-pyridazino[4,5-b]indole (5) and 4-hydrazinopyridazino[4,5-a]indole (12) have been synthesized to investigate their activities as selective thromboxane synthetase inhibitors as well as antihypertensive agents. Several of the prepared compounds were found to be selective thromboxane synthetase inhibitors, in concordance with the Gorman model. The most potent were 8-(benzyloxy)-3,4-dihydro-4-oxo-5H-pyridazino[4,5-b]indole (3c) and 8-methoxy-4-hydrazino-5H-pyridazino[4,5-b]indole (5). This last compound did not inhibit prostacyclin formation and showed an antihypertensive activity similar to that of hydralazine. The acute toxicity in mice for 5a . HCl is about 2.2 times less than that for hydralazine.
Screening Tomato Accessions for Resistance to Xanthomonas campestris pv. vesicatoria, Race T3 (1995)

Tomato ( Lycopersicon esculentum Mill.) accessions were tested for hypersensitivity and rated for resistance following field inoculation with tomato race 3 (T3) of the bacterial spot pathogen Xanthomonas campestris pv. vesicatoria (Doidge) Dye (Xcv) in 1992 and 1993. Hawaii 7981, PI 126932, PI 128216, and selections of the latter two expressed hypersensitivity. Hawaii 7981, only tested in the field in 1993, was nearly symptomless and developed significantly less disease than any other accession. PI 128216 had a level of disease similar to susceptible `Solar Set' when tested in 1993. However, a selection from it (PI 126218-S) was significantly more resistant than `Solar Set' in both years. Although PI 126932 had a level of disease similar to `Solar Set' in both years, a selection from it (PI 126932-1-2) was significantly more resistant than `Solar Set' in 1993. Other accessions without hypersensitive responses but more resistant than `Solar Set' for two seasons were PI 114490, PI 126428, PI 340905-S, and PI 155372. Hawaii 7975 was significantly more resistant than `Solar Set' in the one season it was tested.
Hypertrophic Scarring in Argon Laser Treatment of Port-Wine Stains (1984)

The argon laser (488 to 514 nm) is being used with increasing frequency in the treatment of port-wine stains (PWS). Good results are reported, but scarring is a major complication in all series. The purpose of this paper is to describe the incidence and extent of scarring in a more definitive fashion by quantitating this complication in terms of size, elevation, and color in a consecutive series of 146 patients followed for over 1 year. Scars were assigned a severity score of 1 through 5. A midstudy evaluation led to a significant change in treatment methodology. Therefore, the patient population was divided into two groups. Group I consisted of the first 73 patients and included 30 males and 43 females with an age range of 7 to 81 years (M = 35 years). Group II consisted of the next 73 consecutive patients and included 24 males and 49 females with an age range of 12 to 75 years (M = 43 years). Group II received a different treatment technique than group I. In group I the incidence of scarring was 38 percent for patients under 12 years and 21 percent for patients over 12 years. In group II the incidence was 13.7 percent with concomitant reduction in severity. Statistical analysis (M- HX2 ) compared group I children and adults, P = 0.36; children and group II adults, P = 0.08; group I adults and group II adults, P = 0.42. The most frequent and severe scarring occurred on the upper lip and central portion of the face. Individualization of energy fluency and attention to unique patient factors in healing are important in improving results.
Relative Efficacy of Active Site-Blocked Factors IXa, Xa in Models of Rabbit Venous and Arterio-Venous Thrombosis (1997)

In order to investigate the respective roles of prothrombinase and intrinsic tenase (IXa/VIIIa) in venous thrombosis, we compared the anti-thrombotic efficacy of inhibitors of these two coagulation complexes. The agents tested were dansyl-Glu-Gly-Arg chloromethyl ketone-inactivated bovine factor IXa(IXai) and Glu-Gly-Arg chloromethyl ketone-inactivated human factor Xa (Xai). In vitro formation of active complexes (prothrombinase or tenase) was inhibited by Xai and IXai resulting in IC50 values of 3 nM and 5 nM, respectively. Antithrombotic activity was measured by inhibition of clot accretion on cotton threads placed in the abdominal vena cava of anesthetized rabbits. Intravenous bolus dosing followed by infusion of Xai during the experimental protocol resulted in a dose dependent reduction of clot weight, a dosage of 16.0 micrograms/kg + 0.28 microgram/kg/min being sufficient to produce a 96% inhibition of thrombosis. A much higher dose of IXai (1.0 mg/kg + 17.3 micrograms/kg/min) resulted in a 39% reduction of clot weight. In a rabbit arterio-venous shunt model mimicking arterial thrombosis, the relative efficacy of the two agents was found to be more comparable. The doses required for optimum antithrombotic activity were 128.0 micrograms/kg + 2.2 micrograms/kg/min for Xai and 1.0 mg/kg + 17.3 micrograms/kg/min for IXai. We conclude that, in this study, prothrombinase rather than tenase inhibition was more effective in reducing venous thrombosis and that these effects can be achieved without disruption of extravascular hemostasis.
Installation Effects of a Propeller Mounted on a Wing with Coanda Flap. Part II: Numerical Investigation and Experimental Validation (2014)

Within the framework of the German project B�ürgernahes Flugzeug" (Citizen Friendly Aircraft), we are investigating the effect of installation on propeller sound. A nine-bladed&#13;\npropeller installed in front of a wing equipped with a Coanda flap is considered. This configuration produces significant high lift and thereby considerably influences the inflow condition of the propeller. The sound generated by the propeller is furthermore shielded by and reflected off the wing. In this contribution, we present an implementation of an acoustic propeller model in a �finite differences CAA (computational aeroacoustics) code.&#13;\nComparisons of isolated propeller computations in a quiescent flow with an analytical model illustrate the correct numerical implementation. The verification of the implementation is presented for an example of an isolated propeller. A comparison for the installed propeller&#13;\nwith experiments is conducted for different settings.
Surface-Enhanced Raman Scattering Imaging of Photopatterned Self-Assembled Monolayers (1996)

A nonroughened evaporated silver film was used as a substrate for a self-assembled monolayer of p-nitrothiophenol, which was then subjected to UV photopatterning. The photopattern was imaged using surface-enhanced Raman scattering (SERS). To our knowledge, this is the first report of the use of SERS to image a patterned monolayer. Although the nature of the photoproduct is uncertain, it is probable that it is predominantly the azobenzene species.
Production of anti-tumor human monoclonal antibodies using different approaches (1993)

The production of anti-tumor human monoclonal antibodies (MAbs) by human-human or human-mouse hybridoma technology was studied. UC729-6, a human lymphoblastoid cell line, or NS-1, a mouse myeloma cell line, were fused with lymphocytes isolated from regional lymph modes of 26 patients with breast or gastrointestinal cancer, resulting in 130 immunoglobulin-secreting human-human hybrids and 21 human-mouse hybrids. The supernatants of 88 hybrids were screened against a panel of cancer cells. The supernatants of 37 human-human hybrids and 2 human-mouse hybrids reacted with cancer cell lines. After three times subcloning, only one anti-breast cancer hybrid human MAb, IgG(lambda) human-human hybridoma (MUBL-6), and one anti-gastric cancer human MAb, IgM(lambda) human-mouse hybridoma (HMG-1), were obtained. The antibody-secreting level was 1-4 micrograms/ml/24 h. Production of anti-breast cancer human MAbs by Epstein-Barr virus (EBV) hybridoma was also studied. Human lymphocytes were derived from draining lymph nodes of a breast cancer patient, whose serum antibody strongly reacted with tumor associated antigen (TAA). The enriched B cells were transformed with EBV in vitro. Positive antibody-secreting B cells were selected, expanded, and fused with heteromyeloma SHMD-33. The fusion frequency was 28/10(7) lymphocytes. Among them were 16 hybridomas secreting human immunoglobulin. After subcloning, 60% of the cloned hybridomas kept their antibody-secreting ability. Six observed hybridomas remained stable for more than 1 year in tissue cultures. The antibody-secreting level was 2.9-30 micrograms/ml/24 h. Supernatants from these hybridomas all reacted with breast cancer cell lines but not with gastric cancer cell lines.(ABSTRACT TRUNCATED AT 250 WORDS)
Isolation of multipotent mesenchymal stem cells from umbilical cord blood (2003)

It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different connective tissue lineages such as bone, cartilage, and fat, and little success has been reported in the literature about the isolation of such cells from cord blood. Here we report a novel method to obtain single cell-derived, clonally expanded mesenchymal stem cells that are of multilineage differentiation potential by negative immunoselection and limiting dilution. The immunophenotype of these clonally expanded cells is consistent with that reported for bone marrow mesenchymal stem cells. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, and fat. Surprisingly, these cells were also able to differentiate into neuroglial- and hepatocyte-like cells under appropriate induction conditions and, thus, these cells may be more than mesenchymal stem cells as evidenced by their ability to differentiate into cell types of all 3 germ layers. In conclusion, umbilical cord blood does contain mesenchymal stem cells and should not be regarded as medical waste. It can serve as an alternative source of mesenchymal stem cells to bone marrow.
Effect of cholera toxin on vaccine-induced immunity and infection in murine schistosomiasis mansoni (1993)

Intradermal vaccination of mice with soluble adult worm antigen (SWAP) in combination with Mycobacterium bovis BCG (Swedish strain) induced significant protection against subsequent infection with Schistosoma mansoni cercariae. When cholera toxin (CT) was used as an adjuvant in combination with SWAP or fraction A, no significant protection was observed. However, intradermal vaccination in combination with CT triggered a strong anti-SWAP antibody response and induced a strong delayed-type hypersensitivity response to schistosome antigens (SWAP or fraction A), one significantly higher than that in the SWAP-BCG group. In addition, vaccinating mice intranasally with SWAP or cercarial antigen together with CT as adjuvant failed to induce any significant protection. Surprisingly, mice given CT alone intranasally revealed a significantly enhanced worm burden. These findings suggest that mucosal application of CT may modulate the host-parasite relationship in favor of parasite survival.
A new method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte (2012)

Surface proteins preparations were very reproducible and identification of proteins by mass spectrometry has been demonstrated to be feasible and effective.
Insulin receptor protein-tyrosine phosphatases. Leukocyte common antigen-related phosphatase rapidly deactivates the insulin receptor kinase by preferential dephosphorylation of the receptor regulatory domain. (1992)

A number of protein-tyrosine phosphatase(s) (PTPases) have been shown to dephosphorylate the insulin receptor in vitro; however, it is not known whether any individual PTPase has specificity for certain phosphotyrosine residues of the receptor that regulate its intrinsic tyrosine kinase activity. We evaluated the deactivation of the insulin receptor kinase by three candidate enzymes that are expressed in insulin-sensitive rat tissues, including the receptor-like PTPases LAR and LRP, and the intracellular enzyme, PTPase1B. Purified insulin receptors were activated by insulin and receptor dephosphorylation, and kinase activity was quantitated after incubation with recombinant PTPases from an Escherichia coli expression system. When related to the level of overall receptor dephosphorylation, LAR deactivated the receptor kinase 3.1 and 2.1 times more rapidly than either PTPase1B or LRP, respectively (p less than 0.03). To assess whether these effects were associated with preferential dephosphorylation of the regulatory (Tyr-1150) domain of the receptor beta-subunit, we performed tryptic mapping of the insulin receptor beta-subunit after dephosphorylation by PTPases. Relative to the rate of initial loss of 32P from receptor C-terminal sites, LAR dephosphorylated the Tris-phosphorylated Tyr-1150 domain 3.5 and 3.7 times more rapidly than either PTPase1B or LRP, respectively (p less than 0.01). The accelerated deactivation of the insulin receptor kinase by LAR and its relative preference for regulatory phosphotyrosine residues further support a potential role for this transmembrane PTPase in the physiological regulation of insulin receptors in intact cells.
Evidence for phosphatidylinositol 3-kinase as a regulator of endocytosis via activation of Rab5. (1995)

Phosphatidylinositol (PI) 3-kinases have been implicated in several aspects of intracellular membrane trafficking, although a detailed mechanism is yet to be established. In this study we demonstrated that wortmannin, a specific inhibitor of PI 3-kinases, inhibited constitutive endocytosis of horseradish peroxidase and transferrin in BHK-21 and TRVb-1 cells. The IC50 was approximately 40 ng/ml (93 nM). In addition, wortmannin blocked the stimulation of horseradish peroxidase uptake by the small GTPase Rab5 but not the stimulation by the GTPase-defective, constitutively activated Rab5 Gln79-->Leu mutant (Rab5:Q79L), providing further evidence that PI 3-kinase activity is essential for the early endocytic process. To further investigate the mechanism, we examined the effect of wortmannin on early endosome fusion in vitro. Wortmannin decreased endosome fusion by 80% with an IC50 value similar to that in intact cells. Addition of Rab5:Q79L but not wild-type Rab5 reversed the inhibitory effect of wortmannin. Furthermore, addition of a constitutively activated PI 3-kinase but not its inactive counterpart stimulated early endosome fusion in vitro. These results strongly indicate that PI 3-kinase plays an important role in regulation of early endosome fusion, probably via activation of Rab5.