gebhart/patrepeval huggingface dataset:
Phosphatase Catalysis Developed via Combinatorial Organic Chemistry (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphatase Catalysis Developed via Combinatorial Organic ChemistryF. M. Menger, A. V. Eliseev, and V. A. MigulinCite this: J. Org. Chem. 1995, 60, 21, 6666–6667Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/jo00126a008https://doi.org/10.1021/jo00126a008research-articleACS PublicationsRequest reuse permissionsArticle Views437Altmetric-Citations90LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Serum cytokine and pro-brain natriuretic peptide (BNP) levels in patients with migraine. (2011)

Migraine patients have higher serum IL-1beta and IL-6 levels, and lower IL-10 levels than healthy subjects. These findings support that cytokines may be related to neurogenic inflammation in the pathogenesis of migraine. Also, increased pro-BNP may indicate to preclinical cardiac involvement in patients with migraine.
Errors in Implied Volatility Estimation (2003)

Estimating implied volatility by inverting the Black-Scholes formula is subject to considerable error when option characteristics are observed with plausible errors. Especially for options away from the money, large changes in volatility produce small changes in option prices. Conversely, small errors in option prices and other option characteristics produce large errors in implied volatilities. In the presence of small measurement errors, unobserved truncation of option prices that violate lower bounds for absence of arbitrage can also lead to systematic volatility smiles. The paper proposes feasible GLS estimators that reduce the noise and bias in implied volatility estimates.
The DsbA‐DsbB system affects the formation of disulfide bonds in periplasmic but not in intramembraneous protein domains (1993)

The DsbA and DsbB proteins of Escherichia coli are involved in facilitating the formation of disulfide bonds in periplasmic proteins. Here, we show that the rate of formation of a disulfide bond in the periplasmic domain of the inner membrane protein leader peptidase is reduced in dsbA and dsbB strains, whereas the rate of formation of a disulfide bond engineered into the membrane embedded domain of the same protein is completely unaffected by these mutations. We conclude that the Dsb proteins do not facilitate the formation of intramembraneous disulfides.
Predicting Flu Trends using Twitter data (2011)

Reducing the impact of seasonal influenza epidemics and other pandemics such as the H1N1 is of paramount importance for public health authorities. Studies have shown that effective interventions can be taken to contain the epidemics if early detection can be made. Traditional approach employed by the Centers for Disease Control and Prevention (CDC) includes collecting influenza-like illness (ILI) activity data from “sentinel” medical practices. Typically there is a 1-2 week delay between the time a patient is diagnosed and the moment that data point becomes available in aggregate ILI reports. In this paper we present the Social Network Enabled Flu Trends (SNEFT) framework, which monitors messages posted on Twitter with a mention of flu indicators to track and predict the emergence and spread of an influenza epidemic in a population. Based on the data collected during 2009 and 2010, we find that the volume of flu related tweets is highly correlated with the number of ILI cases reported by CDC. We further devise auto-regression models to predict the ILI activity level in a population. The models predict data collected and published by CDC, as the percentage of visits to “sentinel” physicians attributable to ILI in successively weeks. We test models with previous CDC data, with and without measures of Twitter data, showing that Twitter data can substantially improve the models prediction accuracy. Therefore, Twitter data provides real-time assessment of ILI activity.
Molecular cloning and expression of a cDNA for human kidney cysteine conjugate β‐lyase (1995)

Kidney cysteine conjugate beta-lyase (glutamine transaminase K, kyneurenine aminotransferase, EC 2.6.1.64) metabolises the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites which can produce nephrotoxicity and neurotoxicity in experimental animals and man. Using a combination of hybridisation screening and PCR techniques we have isolated a full-length cDNA for human kidney cysteine conjugate beta-lyase. Comparison of the deduced amino acid sequence with that of the rat enzyme indicated an 82% overall similarity, with 90% similarity around the pyridoxal phosphate binding site, many of the changes being conservative in nature. Expression of the cDNA in Cos-1 cells resulted in the production of a cytosolic enzyme which showed both cysteine conjugate beta-lyase and glutamine transminase K activity. Preliminary mapping of the gene for human cysteine conjugate beta-lyase by PCR analysis of genomic DNA from human-rodent hybrid cells indicated that it is located on human chromosome 9.
Identification of fatty acid binding proteins as markers associated with the initiation and/or progression of renal cell carcinoma (2005)

Renal cell carcinoma (RCC) representing the most common neoplasia of the kidney in Western countries is a histologic diverse disease with an often unpredictable course. The prognosis of RCC is worsened with the onset of metastasis, and the therapies currently available are of limited success for the treatment of metastatic RCC. Although gene expression analyses and other methods are promising tools clarifying and standardizing the pathological classification of RCC, novel innovative molecular markers for the diagnosis, prognosis, and for the monitoring of this disease during therapy as well as potential therapeutic targets are urgently needed. Using proteome-based strategies, a number of RCC-associated markers either over-expressed or down-regulated in tumor lesions in comparison to the normal epithelium have been identified which have been implicated in tumorigenesis, but never linked to the initiation and/or progression of RCC. These include members of the fatty acid binding protein family, which have the potential to serve as diagnostic or prognostic markers for the screening of RCC patients.
Immunologic Role of the Cholinergic Anti‐Inflammatory Pathway and the Nicotinic Acetylcholine α7 Receptor (2005)

Cytokines are small protein molecules that facilitate communication between cells of the immune system and other tissues. Their messenger function elicits responses in other cells, ranging from DNA binding and tissue remodeling to coordinating the local cellular response to inflammation. The magnitude of the cytokine response is regulated closely, because an over- or underabundance of cytokine activity can impair organ function and cause shock and tissue injury. Counterregulatory molecular and humoral mechanisms protect the host from cytokine excess, including the pituitary-adrenal-glucocorticoid system, and the anti-inflammatory cytokine system. These humoral systems are protective, but they are relatively slow-acting, concentration-gradient dependent, and not integrated. We recently discovered that the "cholinergic anti-inflammatory pathway," a nervous system-based, rapid, and locally acting mechanism, can inhibit the cytokine response. Signals transmitted via the vagus nerve converge on cytokine-producing cells that express the nicotinic acetylcholine receptor alpha 7 (nAChR alpha7). The alpha7 receptor is an essential component of the cholinergic anti-inflammatory pathway, because activation of this receptor prevents cytokine release. Advances in understanding the molecular structure and function of alpha7 have begun to provide a better understanding of potential mechanisms regarding its assembly, expression, and cytokine-inhibiting functions. It may be possible to exploit this pathway to therapeutic advantage for diseases caused by excessive cytokine activity.
Stabilization of an α-helix by short adjacent accessory foldamers (2016)

Template-based stabilization of α-peptide helices with short accessory non-peptide helical foldamers fused either at the N- or C-terminus or at both ends of the peptide segment has been investigated by NMR spectroscopy in polar solvents and by X-ray diffraction. In this work, we focused on aliphatic N,N′-linked oligoureas that form predictable and well-defined helical structures akin to α-helices. Our results indicate that urea oligomers have the ability to enforce a peptide segment to adopt a well-defined α-helical structure and may suggest a general approach to stabilize short helical peptide epitopes for the development of modulators of protein–protein interactions.
Prehospital Transfusion of Plasma and Red Blood Cells in Trauma Patients (2014)

Abstract Objective. Earlier use of plasma and red blood cells (RBCs) has been associated with improved survival in trauma patients with substantial hemorrhage. We hypothesized that prehospital transfusion (PHT) of thawed plasma and/or RBCs would result in improved patient coagulation status on admission and survival. Methods. Adult trauma patient records were reviewed for patient demographics, shock, coagulopathy, outcomes, and blood product utilization from September 2011 to April 2013. Patients arrived by either ground or two different helicopter companies. All patients transfused with blood products (either pre- or in-hospital) were included in the study. One helicopter system (LifeFlight, LF) had thawed plasma and RBCs while the other air (OA) and ground transport systems used only crystalloid resuscitation. Patients receiving PHT were compared with all other patients meeting entry criteria to the study cohort. All comparisons were adjusted in multilevel regression models. Results. A total of 8,536 adult trauma patients were admitted during the 20-month study period, of which 1,677 met inclusion criteria. They represented the most severely injured patients (ISS = 24 and mortality = 26%). There were 792 patients transported by ground, 716 by LF, and 169 on OA. Of the LF patients, 137 (19%) received prehospital transfusion. There were 942 units (244 RBCs and 698 plasma) placed on LF helicopters, with 1.9% wastage. PHT was associated with improved acid-base status on hospital admission, decreased use of blood products over 24 hours, a reduction in the risk of death in the sickest patients over the first 6 hours after admission, and negligible blood products wastage. In this small single-center pilot study, there were no differences in 24-hour (odds ratio 0.57, p = 0.117) or 30-day mortality (odds ratio 0.71, p = 0.441) between LF and OA. Conclusions. Prehospital plasma and RBC transfusion was associated with improved early outcomes, negligible blood products wastage, but not an overall survival advantage. Similar to the data published from the ongoing war, improved early outcomes are associated with placing blood products prehospital, allowing earlier infusion of life-saving products to critically injured patients.
Transplantation in humans of encapsulated xenogeneic cells without immunosuppression. A preliminary report. (1994)

Keywords: Adrenal Medulla/cytology ; Animals ; Cattle ; Cell Transplantation/methods ; Humans ; Immune Tolerance ; Prostheses and Implants ; Transplantation Immunology ; Transplantation ; Heterologous Note: Department of Neurosurgery Centre Hospitalier Universitaire Vaudois University of Lausanne Medical School, Switzerland. Reference LEN-ARTICLE-1994-004 Record created on 2007-03-09, modified on 2017-05-12
Characterization of four homologous L chain variable region genes that are related to 6B6.6 idiotype positive human rheumatoid factor L chains. (1989)

A significant proportion of monoclonal IgM rheumatoid factors (RF) from patients with mixed cryoglobulinemia express a KIIIa L chain-associated cross-reactive idiotype, termed 6B6.6. Previously, we reported the isolation from a patient with a monoclonal RF of a Vk gene, termed Humkv328, whose deduced amino acid sequence differs by three and seven residues from the L chains of the 6B6.6-positive RF Les and Pom, respectively. To further delineate the genetic basis of the 6B6.6 cross-reactive idiotype, we isolated from the same patient another Vk gene, termed Humkv329, which differs from kv328 by only three bases over a stretch of 1331 nucleotides sequenced. However, kv329 has a stop codon at amino acid position 94, and is thus a pseudogene. Therefore, we screened a second genomic library from an unrelated individual and isolated two potential function Vk genes (i.e., Humkv328h2 and Humkv328h5) which are highly homologous to Humkv328. All three potential functional Vk genes differ from each other by one to six bases over a 1331-bp stretch and all encode the same Vk region amino acid sequence. Among the six bases by which Humkv328h5 differs from Humkv328, two are in the conserved pentadecanucleotide region, which is known to be important in the regulation of k L chain transcription. In the future it will be important to ascertain the potential association of polymorphisms in the conserved pentadecanucleotide region with RF associated autoimmune and lymphoproliferative diseases.
Identification and Screening of 416 Patients with Chronic Hepatitis at High Risk to Develop Hepatocellular Cancer (1995)

This screening study in patients with chronic HBV or HCV infection demonstrates (1) that the yield of asymptomatic hepatocellular cancer on initial screening is 7.9% and (2) that patients with severe chronic active hepatitis, cirrhosis, or both are at extremely high risk to develop hepatocellular cancer (25%). On the basis of these results and the finding of a significant number of small; treatable hepatocellular cancers (61.1%), the authors recommend hepatocellular cancer screening every 3 months for the subset of high-risk patients.
Precoding for multicell massive MIMO systems with compressive rank-q channel approximation (2013)

In non-cooperative multicell massive (or very large) multiple-input multiple-output (MIMO) systems, the pilot contamination effect in the uplink channel training and the intercell interference in the downlink severely degrade the overall network achievable rates. In this paper, we present a framework to mitigate those effects. Specifically, we propose in the uplink training a rank-q channel approximation method based on compressive sensing (CS) to estimate the most dominant singular subspaces of the global multicell MIMO channel matrix with a modest training length. Then, the estimate of the global channel information is used to design an intercell-interference-aware (IA) zero-forcing (ZF) multicell precoding method in the downlink to mitigate not only the intracell interference but also the intercell interference of the channel. The results obtained using the proposed scheme show a significant improvement in the achievable rates for all users in the cells, particularly the cell-edge users, as compared to the existing channel estimation and precoding methods.
Enhancement of immunogenicity of HPV16 E7 oncogene by fusion with E. coli β‐glucuronidase (2004)

The findings demonstrate the superior immunisation efficacy of the fusion genes of E7GGG with GUS when compared with E7GGG and Sig/E7GGG/LAMP-1. The E7GGG.GUS-based DNA vaccine might also be efficient against human tumour cells with reduced MHC class I expression.
Regulation of alternative splicing in the IIICS region of human fibronectin pre-mRNA encoding cell binding sites CS1 and CS5 (1992)

The cell binding sites CS1 and CS5 in the IIICS region of human fibronectin (FN) mediate the adhesion of specific cell types by interacting with the integrin alpha 4 beta 1. IIICS pre-mRNA is alternatively spliced via the use of three alternative splice acceptor sites and one alternative splice donor site. These alternative splicing pathways can potentially give rise to variant FN molecules which are CS1+,CS5+; CS1+,CS5-; CS1-,CS5+ or CS1-,CS5-. Here we show that selection of the acceptor site which incorporates mRNA encoding CS1 and CS5 is more frequent in foetal tissues compared to adult liver, whereas an alternative acceptor site and the alternative donor site, which exclude CS1 and CS5, are used at a higher level in adult liver compared to foetal tissue. All possible splice junctions were accurately processed, and selected at different levels in mRNA expressed from a IIICS minigene transiently transfected into a HeLa cell line which does not express FN, suggesting that all the cellular factors required for alternative processing of IIICS are present in this system. Furthermore, pre-mRNA expressed from a mutant construct lacking IIICS-1 intron sequence, was correctly processed in HeLa cells via selection of all possible splice sites. On the basis of our results we propose that regulation of splice site selection in IIICS and thus expression of CS1 and CS5 is achieved by subtle tuning of splicing systems involving the interaction of local cis elements and cellular factors which are not necessarily restricted developmentally or tissue-specifically, and that expression of CS1 and CS5 is independently regulated.
Geohazard risk management for infrastructure projects (2006)

This paper presents generic guidance for construction professionals for the assessment of geological-related hazards and risks for infrastructure projects. The risks presented by such hazards to a project are dependent upon their potential size and impact. Drawing on practical experience of civil engineering projects in the UK and worldwide, the authors show that, through a carefully planned risk management framework, the potential impact of uncertain events related to geohazards can be identified, appropriately investigated and mitigated. The corresponding reduction in uncertainty provides improved control over both costs and programme to the advantage of all parties involved.
Intracellular trafficking of proteins (1991)

List of contributors Foreword J. L. GOLDSTEIN and M. S. BROWN Preface C. J. STEER and J. A. HANOVER Introduction X. CINADER I ENDOCYTOSIS Overview M. C. WILLINGHAM and I. PASTAN 1. Molecular motors that shape endocytic membrane R. G. W. ANDERSON 2. Clathrin coated pits and coated vesicles: involvement in endo- and exocytosis C. J. STEER and J. HEUSER 3. Dissection of endosomes P. J. COURTOY 4. Acidification of organelles and the intracellular sorting of proteins during endcytosis F. J. MAXFIELD and D. J. YAMASHIRO 5. Intracellular routing and membrane translocation of diphtheria toxin and ricin V. G. JOHNSON and R. J. YOULE 6. Pseudomonas exotoxin and derived conjugates: interactions with mammalian cells D. FITZGERALD and I. PASTAN 7. Intracellular pathways of immunoglobulin A and epidermal growth factor transport in hepatocytes S. J. BURWEN and A. L. JONES 8. The asialoglycoprotein receptor: intracellular fate of ligand and receptor A. L. SCHWARTZ, H. J. GEUZE and G. J. A. M. STROUS 9. Structure, function and regulation of the transferrin receptor: insights from molecular biology J. B. HARFORD, J. L. CASEY, D. M. KOELLER and R. D. KLAUSNER 10. Autocrine targeting of hormones R. B. DICKSON II THE EXOCYTIC PATHWAY Overview W. J. LENNARZ 11. Intersection of endocytic and exocytic membrane traffic in animal cells M. D. SNIDER 12. The role of the endoplasmic reticulum in nascent chain translocation: a perspective from the study of fusion proteins V. R. LINGAPPA 13. Protein traffic through the Golgi complex M. G. FARQUHAR 14. Biosynthesis and processing of lysomal membrane glycoproteins L. M. ARTERBURN, D. A. McCLELLAN and J. T. AUGUST 15. Trafficking of secreted proteins A. A. McCRACKEN 16. The T-cell antigen receptor: assembly and expression of a multisubunit complex J. S. BONIFACINO, J. LIPPINCOTT-SCHWARTZ and R. D. KLAUSNER 17. The polarized transport of surface proteins and lipids in epithelial cells A. WANDINGER-NESS and K. SIMONS III PROTEIN TRANSPORT INTO NUCLEI, MITOCHONDRIA AND PEROXISOMES Overview J. A. HANOVER and C. J. STEER 18. The nuclear pore and nucleocytoplasmic exchange B. WOLFF, M. K. PARK and J. A. HANOVER 19. Mitochondrial import of proteins M. G. DOUGLAS, C. S. SMAGULA and W-J. CHEN 20. Translocation of proteins into peroxisomes S. J. GOULD and S. SUBRAMANI
Crystal Structure of Recombinant Native SDF-1α with Additional Mutagenesis Studies: An Attempt at a More Comprehensive Interpretation of Accumulated Structure-Activity Relationship Data (2000)

Crystal structures, forms 1 and 2, of recombinant native stromal cell-derived factor-1alpha (SDF-1alpha), expressed using the Sendai virus expression vector system, have been determined by x-ray crystallography at 2.0 A resolution. The crystal of form 1 is almost isomorphous with that used in the previous crystal structure analysis of the synthetic [N33A] mutant of SDF-1alpha (Dealwis, C., et al. Proc. Natl. Acad. Sci. USA 1998;95, 6941-6946). However, the present structure analysis led to considerably better refinement statistics, revealing an error in the structural assignment of N-terminal residues in the previous report. Comparison of the solution structure, as previously determined by nuclear magnetic resonance (NMR) spectroscopy, and the present structure, with two monomers in the asymmetric unit, reveals several local conformational differences. Alanine scan mutagenesis studies for each residue in the so-called RFFESH motif revealed that only the first residue, Arg12, is effective in enhancing receptor binding (and successive activation). A new notion that steric restraint between Arg8 and Arg12 is favorable (if not vital) for retaining SDF activities appears to explain more consistently the structure-activity relationship data accumulated to date. Four guiding principles are presented that may be useful for designing potent therapeutic compounds interfering with HIV-1 infection through competition at the CXCR4 coreceptor.