gebhart/patrepeval huggingface dataset:
Urodynamic studies of women with prolapse and stress incontinence before and after surgical repair. (1979)

Twenty-one women, 11 suffering from both prolapse and stress incontinence and 10 with prolapse only were investigated before and at different intervals after surgical repair of the disorders. The investigations comprised a gynaecological examination, urine culture, observation of residual urine and simultaneous urethro-cystometry including measurement of the urethral pressure profile.--The prolapse patients were operated upon by conventional Manchester technique. The patients suffering from both prolapse and stress incontinence were operated with a combined vaginal-abdominal repair using Lyodura slings.--It was found that the pre-operative urethral pressure at rest was lower in the inconinent-prolapse patients compared with that in patients suffering from prolapse only. After surgery the urethral pressure at rest was significantly decreased in the incontinent-prolapse patients whereas it did not change in patients operated because of prolaspe only.--Prior to the operation, all incontinent-prolapse women had a negative urethral closure pressure at stress. After surgery the closure pressure became positive in all patients and none complained about urinary incontinence. In all patients suffering from prolapse only the urethral closure pressure was positive at stress before as well as after surgery. The functional and the absolute urethral lengths increased in both categories of patients after the operation.
A new continuous‐flow cell separation method based on cell density: Principle, apparatus, and preliminary application to separation of human buffy coat (2001)

With the recent progress in transfusion medicine, separation and isolation of cells in a large quantity is becoming increasingly important. At present, the continuous cell separation method in a preparative scale is limited to apheresis and elutriation: the former is mainly used for collection of platelet and buffy coat from the whole blood, while the latter separates cells virtually according to their size. Here we introduce a continuous flow method that separates cells entirely based on cell density. The method is gentle and capable of processing a large number of cells. The potential capability of the method was demonstrated on separation of lymphocytes and granulocytes from human buffy coat. Lymphocytes were enriched to 90% in the fraction at density = 1.065 and granulocytes are isolated in fractions at density = 1.075-1.080 while red cells were completely retained at the periphery of the channel. CD34 cells were distributed around 1.065 and coeluted with lymphocytes, suggesting that further enrichment requires focusing the density gradient around 1.070. The method could process 10(9) nucleated cells in 2 hours. Our preliminary results suggest that the present method is an effective and efficient means to separate blood cells.
Wide-area parallel programming using the remote method invocation model (2000)

Java's support for parallel and distributed processing makes the language attractive for metacomputing applications, such as parallel applications that run on geographically distributed (wide-area) systems. To obtain actual experience with a Java-centric approach to metacomputing, we have built and used a high-performance wide-area Java system, called Manta. Manta implements the Java Remote Method Invocation (RMI) model using different communication protocols (active messages and TCP/IP) for different networks. The paper shows how wide-area parallel applications can be expressed and optimized using Java RMI. Also, it presents performance results of several applications on a wide-area system consisting of four Myrinet-based clusters connected by ATM WANs. We finally discuss alternative programming models, namely object replication, JavaSpaces, and MPI for Java. Copyright © 2000 John Wiley & Sons, Ltd.
Core-based scan architecture for silicon debug (2003)

In this paper, we present a core-based scan architecture for silicon debug, which is currently being standardized within Philips. The reasons behind the core-based debug architecture, together with implementation details, are described. The choices that were made during its development are explained using the experiences gained from two large Philips system chips that each utilize core-based design and test, and scan-based silicon debug. The results of an area-cost evaluation of the presented architecture for these two large system chips are also presented.
Universal data access with OLE DB (2002)

OLE DB is Microsoft's new data access API designed to enable access to all kinds of data sources, both database and non database, regardless of format or location. OLE DB builds on Microsoft's Component Object Model (COM), which is the foundation for OLE and ActiveX. OLE DB is core to Microsoft's Universal Access strategy. OLE DB aims at providing an environment for business applications to access all kinds of data sources in an integrated way, including desktop data such as spreadsheets, text processing documents, and electronic mail; server data stored in the file system, indexed sequential files, relational, hierarchical and network databases; and data computed by middle tier business objects. Most database companies are pursuing a Universal Storage strategy which provides access to all kinds of data types such as text, spatial, video, and audio, and insist on placing all kinds of data used in an organization inside the database. However, the reality is that a vast amount of mission critical data in a corporation is stored in a combination of database and non database data sources for functionality and performance reasons. Therefore, Microsoft is pursuing a Universal Access strategy which provides an infrastructure that enables the integration of a wide variety of data sources so that applications can be written in an efficient, safe and disciplined manner without losing the advantages of a centralized database system. The paper provides an overview of OLE DB and describes how OLE DB enables the Microsoft Universal Access approach to managing data.
Integrin-Mediated Drug Resistance in Multiple Myeloma (2000)

Drug resistance remains a major obstacle to the treatment of many hematopoietic malignancies such as multiple myeloma. Although much research has been focused on acquired resistance phenotypes, we believe that de novo drug resistance mechanisms may be an important component in protecting cells from initial drug exposure. It is now realized that many of the biological processes associated with this disease, including cell survival, may come as a result of the direct interactions of malignant plasma cells with the bone marrow microenvironment. This review examines the role of cell adhesion to one bone marrow component, fibronectin (FN), and the impact it may have on response to cytotoxic drugs. We discuss the influence of the integrin VLA-4 (alpha4beta1) on cell adhesion mediated drug resistance (CAM-DR) as well as the effects of chronic drug exposure on integrin function. Data presented here demonstrates that drug selection can make a non-adherent cell line adherent to FN through inside-out integrin activation and consequently cause a decrease in sensitivity to drug. We also speculate on the possible mediators of this intrinsic mechanism of drug resistance which may, along with the integrins themselves, become promising therapeutic targets in cancer treatment.
Bone morphogenetic proteins induce the expression of noggin, which limits their activity in cultured rat osteoblasts. (1998)

Bone morphogenetic proteins (BMPs) induce the differentiation of cells of the osteoblastic lineage and enhance the function of the osteoblast. Growth factors are regulated by binding proteins, but there is no information about binding proteins for BMPs in skeletal cells. Noggin specifically binds BMPs, but its expression by cells of the osteoblastic lineage has not been reported. We tested for the expression of noggin and its induction by BMP-2 in cultures of osteoblast-enriched cells from 22-d-old fetal rat calvariae (Ob cells). BMP-2 caused a time- and dose-dependent increase in noggin mRNA and polypeptide levels, as determined by Northern and Western blot analyses. The effects of BMP-2 on noggin transcripts were dependent on protein, but independent of DNA synthesis. BMP-2 increased the rates of noggin transcription as determined by nuclear run-on assays. BMP-4, BMP-6, and TGF-beta1 increased noggin mRNA in Ob cells, but basic fibroblast growth factor, platelet- derived growth factor BB, and IGF-I did not. Noggin decreased the stimulatory effects of BMPs on DNA and collagen synthesis and alkaline phosphatase activity in Ob cells. In conclusion, BMPs induce noggin transcription in Ob cells, a probable mechanism to limit BMP action in osteoblasts.
Performance and limitations of steatosis biomarkers in patients with nonalcoholic fatty liver disease (2014)

All five steatosis biomarkers can diagnose steatosis and are correlated with insulin resistance. They are confounded by fibrosis and inflammation, and do not accurately quantify steatosis; this may limit their clinical utility. More research is needed to identify truly independent and quantitative markers of steatosis.
Distribution of an L-isoaspartyl protein methyltransferase in eubacteria (1992)

A protein carboxyl methyltransferase (EC 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. This enzyme catalyzes the transfer of methyl groups from S-adenosylmethionine to the carboxyl groups of D-aspartyl or L-isoaspartyl residues that are formed spontaneously from normal L-aspartyl and L-asparaginyl residues. A similar methyltransferase has been found in two bacterial species, Escherichia coli and Salmonella typhimurium, suggesting that this enzyme performs an essential function in all cells. In this study, we show that this enzyme is present in cytosolic extracts of six additional members of the alpha and gamma subdivisions of the purple bacteria: Pseudomonas aeruginosa (gamma), Rhodobacter sphaeroides (alpha), and the gamma enteric species Klebsiella pneumoniae, Enterobacter aerogenes, Proteus vulgaris, and Serratia marcescens. DNA probes from the E. coli methyltransferase gene hybridized only to the chromosomal DNA of the enteric species. Interestingly, no activity was found in the plant pathogen Erwinia chrysanthemi, a member of the enteric family, nor in Rhizobium meliloti or Rhodopseudomonas palustris, two members of the alpha subdivision. Additionally, we could not detect activity in the four gram-positive species Bacillus subtilis, B. stearothermophilus, Lactobacillus casei, and Streptomyces griseus. The absence of enzyme activity was not due to the presence of inhibitors in the extracts. These results suggest that many cells may not have the enzymatic machinery to recognize abnormal aspartyl residues by methylation reactions. Since the nonenzymatic degradation reactions that generate these residues occur in all cells, other pathways may be present in nature to ensure that these types of altered proteins do not accumulate and interfere with normal cellular physiology.
Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes (2008)

Vaccinia virus is the prototypic orthopoxvirus and was the vaccine used to eradicate smallpox, yet the expression profiles of many of its genes remain unknown. Using a genome tiling array approach, we simultaneously measured the expression levels of all 223 annotated vaccinia virus genes during infection and determined their kinetics. For 95% of these genes, significant transcript levels were detected. Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression.
Primer Design for PCR and Sequencing in High-Throughput Analysis of SNPs (2002)

To achieve high-throughput analysis of allele frequencies in human SNPs, we have developed automated methodsfor designing PCR and DNA sequencing primers. We found we could run the PCR assays at quite stringent, uniform conditions. The design process used freely available databases, including dbSNP, SNPper, and TSC, and publicly available software including RepeatMasker and Primer3. We describe parameters for the software and other considerations that increase experimental success. As anticipated. some assays filed at the design stage due primarily to the genomic locations of repetitive sequences, extreme GC content regions, or lack of sufficient sequence. However, over 23,000 assays, including 96% of those recently analyzed, have been experimentally successfuL Similar design methods could be usedfor PCR assays in any organism with substantial available sequence.
Effects of Mannose-6-Phosphate on Receptor-Mediated Endocytosis of Insulin-Like Growth Factor-II* (1990)

The insulin-like growth factor-II (IGF-II) and lysosomal enzymes containing a mannose-6-phosphate (M6P) recognition site bind to different sites of the same receptor molecule. We have observed that M6P increases the receptor-mediated uptake of IGF-II into IM-9 cells. We now confirm this phenomenon in a different line, the H-35 rat hepatoma cells, and present additional characterization of the underlying mechanisms. When incubated in the presence of radiolabeled IGF-II, H-35 cells accumulated, in a time-dependent fashion, radioactivity that was resistant to removal by trypsin digestion at 15 C, indicating that it was endocytosed. In the presence of 3 mM M6P, endocytosed counts were approximately 2-fold higher after 5 min of incubation, an enhancement that peaked at 10 min, then declined, but was still evident after 40 min (1.5-fold). The rate of release of cell-associated IGF-II, degraded or intact, as measured in a chase experiment, was not affected by M6P. These observations indicate that M6P increased accumulation of IGF-II by accelerating its rate of endocytosis rather than by interfering with IGF-II degradation or with the recycling of intact hormone-receptor complexes to the cell surface. Electrophoresis after affinity cross-linking of labeled cells demonstrated that the enhancement in radioactivity could be located at a molecular size of approximately 250 kDa, corresponding to IGF-II-receptor complexes. Preincubation with M6P did not significantly alter the specific binding of IGF-II to the cell surface of H-35 cells, as measured by a binding assay at 4 C. Finally, pretreatment with cycloheximide for up to 8 h, to remove all newly synthesized lysosomal enzymes bound to the M6P/IGF-II receptor, did not affect IGF-II endocytosis beyond what could be accounted for by some loss of receptor, suggesting that the observed effect of M6P is due to the binding of M6P itself to the receptor and not to displacement of lysosomal enzymes. We conclude that simultaneous occupancy of the M6P/IGF-II receptor by ligands on both binding sites enhances its rate of endocytosis.
Magnetic sensors and their applications (2006)

Magnetic sensors can be classified according to whether they measure the total magnetic field or the vector components of the magnetic field. The techniques used to produce both types of magnetic sensors encompass many aspects of physics and electronics. Here, we describe and compare most of the common technologies used for magnetic field sensing. These include search coil, fluxgate, optically pumped, nuclear precession, SQUID, Hall-effect, anisotropic magnetoresistance, giant magnetoresistance, magnetic tunnel junctions, giant magnetoimpedance, magnetostrictive/piezoelectric composites, magnetodiode, magnetotransistor, fiber optic, magnetooptic, and microelectromechanical systems-based magnetic sensors. The usage of these sensors in relation to working with or around Earth's magnetic field is also presented.
Purification to apparent homogeneity of a mu-type opioid receptor from rat brain. (1986)

A mu-opioid-specific receptor was purified to apparent homogeneity from rat brain membranes by 6-succinylmorphine affinity chromatography, gel filtration, wheat germ agglutinin affinity chromatography, and isoelectric focusing. The purified receptor had a molecular weight of 58,000 as determined by NaDodSO4/polyacrylamide gel electrophoresis and was judged to be homogeneous by the following criteria: (i) a single band was detected by autoradiography after NaDodSO4/polyacrylamide gel electrophoresis of 125I-labeled receptor and (ii) the purified preparation had a specific opioid-binding activity of 17,720 pmol/mg of protein, close to the theoretical value. In addition, the Mr 58,000 value agrees closely with that determined by covalently labeling purified receptor with bromoacetyl-[3H]dihydromorphine or with 125I-labeled beta-endorphin and dimethyl suberimidate.
Inferring haplotypes at the NAT2 locus: the computational approach (2005)

This investigation provides a solid basis for the confident and rational use of computational methods which appear to be a good alternative to infer haplotype phases in the particular case of the NAT2 gene, where there is near complete linkage disequilibrium between polymorphic markers.
Forages with condensed tannins - their management and nutritive value for ruminants (1998)

Temperate forage legumes that contain condensed tannins (CT) have been evaluated under experimental conditions and some have demonstrated significant benefits for sheep, cattle and deer. Condensed tannins can significantly improve productive performance, as well as lessen our dependence on anthelmintic drenches. However, not all CT are equal and some may not benefit production at all. This paper summarises the performance responses of ruminants to CT in birdsfoot trefoil (Lotus corniculatus), lotus (Lotus pedunculatus), sulla (Hedysarum coronarium), sainfoin (Onobrychis viciifolia) and erect dorycnium (Dorycnium rectum), and indicates the requirements for successful establishment and management of these swards. All require specialist care, and few will compete with common pasture species in high-fertility situations, but the potential benefits of reduced anthelmintic requirements when sheep are fed sulla, and the lowered incidence of flystrike and improved weight gain, wool growth, milk production and ovulation rate attributable to the CT in birdsfoot trefoil, makes these attractive options for farmers. Lotus is a high quality feed for wet, acid, low-fertility soils and sainfoin may result in very good stock performance in dry regions. Annual dry matter yields of the five species outlined here range from about 10 to 25 t/ha and some of these forages will benefit producers who are prepared to manage these forages correctly. Keywords: birdsfoot trefoil, condensed tannin, dorycnium, forage legume, lotus, ruminant nutrition, sainfoin, sulla
Navajo Nation v. Rodriguez and the Traditional Navajo Principle of Hazhó'ógo (2004)

This case note examines in detail the adoption, and adaptation, of the United States Supreme Court's Miranda decision by the Supreme Court of the Navajo Nation. The Supreme Court of the Navajo Nation carried out the directive of the Fundamental Laws of the Diné, to make Diné bi beehaz'áanii, or Navajo Common Law, the fundamental basis for its decisions. By doing so, the Supreme Court defined uniquely Navajo rights and procedures governing the custodial interview, holding that the traditional Navajo principle of hazhó'ógo requires truthful, transparent explanations to, and respectful treatment of, persons in police custody. Mr. Morin's case note goes on to put the Rodriguez opinion into context, demonstrating that the Court's approach fits squarely into both the Court's well-established practice of applying traditional Navajo principles to the resolution of legal disputes and the Court's more recent practice of implementing, wherever appropriate, the directive of the Fundamental Laws of the Diné passed by the Navajo Nation Council in November of 2002.
Le sentiment de la mort nucléaire (1993)

Thomas Louis-Vincent. Le sentiment de la mort nucléaire. In: Communications, 57, 1993. Peurs, sous la direction de Bernard Paillard. pp. 101-120.
Multilineage Potential of Homozygous Stem Cells Derived from Metaphase II Oocytes (2003)

Human stem cells derived from human fertilized oocytes, fetal primordial germ cells, umbilical cord blood, and adult tissues provide potential cell-based therapies for repair of degenerating or damaged tissues. However, the diversity of major histocompatibility complex (MHC) antigens in the general population and the resultant risk of immune-mediated rejection complicates the allogenic use of established stem cells. We assessed an alternative approach, employing chemical activation of nonfertilized metaphase II oocytes for producing stem cells homozygous for MHC. By using F1 hybrid mice (H-2-B/D), we established stem cell lines homozygous for H-2-B and H-2-D, respectively. The undifferentiated cells retained a normal karyotype, expressed stage-specific embryonic antigen-1 and Oct4, and were positive for alkaline phosphatase and telomerase. Teratomatous growth of these cells displayed the development of a variety of tissue types encompassing all three germ layers. In addition, these cells demonstrated the potential for in vitro differentiation into endoderm, neuronal, and hematopoietic lineages. We also evaluated this homozygous stem cell approach in human tissue. Five unfertilized blastocysts were derived from a total of 25 human oocytes, and cells from one of the five hatched blastocysts proliferated and survived beyond two passages. Our studies demonstrate a plausible "homozygous stem cell" approach for deriving pluripotent stem cells that can overcome the immune-mediated rejection response common in allotransplantation, while decreasing the ethical concerns surrounding human embryonic stem cell research.
Imaging with total internal reflection fluorescence microscopy for the cell biologist (2010)

Total internal reflection fluorescence (TIRF) microscopy can be used in a wide range of cell biological applications, and is particularly well suited to analysis of the localization and dynamics of molecules and events near the plasma membrane. The TIRF excitation field decreases exponentially with distance from the cover slip on which cells are grown. This means that fluorophores close to the cover slip (e.g. within ~100 nm) are selectively illuminated, highlighting events that occur within this region. The advantages of using TIRF include the ability to obtain high-contrast images of fluorophores near the plasma membrane, very low background from the bulk of the cell, reduced cellular photodamage and rapid exposure times. In this Commentary, we discuss the applications of TIRF to the study of cell biology, the physical basis of TIRF, experimental setup and troubleshooting.