gebhart/patrepeval huggingface dataset:
Synthesis of N-(phenylsulfonyl)-CI, N-((tert-butyloxy)carbonyl)-CI, CI-CDPI1, and CI-CDPI2: CC-1065 functional analog incorporating the parent 1,2,7,71-tetrahydrocycloprop[1,2-c]indol-4-one (CI) left-hand subunit (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of N-(phenylsulfonyl)-CI, N-((tert-butyloxy)carbonyl)-CI, CI-CDPI1, and CI-CDPI2: CC-1065 functional analog incorporating the parent 1,2,7,71-tetrahydrocycloprop[1,2-c]indol-4-one (CI) left-hand subunitDale L. Boger, Ronald J. Wysocki Jr., and Takayoshi IshizakiCite this: J. Am. Chem. Soc. 1990, 112, 13, 5230–5240Publication Date (Print):June 1, 1990Publication History Published online1 May 2002Published inissue 1 June 1990https://pubs.acs.org/doi/10.1021/ja00169a034https://doi.org/10.1021/ja00169a034research-articleACS PublicationsRequest reuse permissionsArticle Views553Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
On illumination schemes for wide-field CARS microscopy (2009)

New system for a wide-field CARS microscopy is demonstrated, including two schemes of non-phase-matching illumination. Several advantages including high Stokes pulse energy, pulse-to-pulse stability and inherent synchronization between pump and Stokes pulses were brought by use of methane-filled Raman converter. Spatial resolution of the system with axially symmetric illumination, 0.5 microm, was found to correspond to diffraction limit of the imaging objective. Selective sensitivity to lipid-rich myelin sheaths in the nerve tissue has been demonstrated and confirmed by comparison with histological samples stained with myelin-specific dye. Single-shot imaging capability of the system has been demonstrated with a speckling-free illumination on a monolayer of 3 microm polystyrene beads.
Culture environment modulates maturation and metabolism of human oocytes (2002)

We have shown that: (i). pyruvate is a major energy source during oocyte maturation; (ii). the composition of the culture medium can affect the rate of maturation; and (iii). the culture medium and stage of nuclear maturation can affect pyruvate uptake and lactate production.
Sortase A-Catalyzed Site-Specific Coimmobilization on Microparticles via Streptavidin (2012)

A microparticle surface was designed by the unique method incorporating streptavidin-biotin affinity and sortase A (SrtA)-catalyzed transpeptidation. Leucine-proline-glutamate-threonine-glycine-tagged streptavidin (Stav-LPETG)was immobilized on the surface using streptavidin-biotin affinity, and GGGGG-tagged red fluorescent protein (Gly5-RFP) was conjugated with SrtA. Biotinylated fluorescein isothiocyanate (biotin-FITC) was then bound to residual biotin-binding sites in Stav-LPETG. The resulting particles had RFP and FITC immobilized on the surface via Stav-LPETG, and RFP- and FITC-associated fluorescence was observed using fluorescence microscopy. Finally, GGG-tagged glucose oxidase and biotinylated horseradish peroxidase were immobilized on the microparticle surface, resulting in a functional particle capable of detecting glucose. This particle can be repeatedly used and is more sensitive in detecting glucose than particles prepared using chemical modification. Our method provides a simple strategy for site-specific coimmobilization on molecular surfaces and expands the use of protein hybrid devices.
Sacral nerve stimulation for faecal incontinence and constipation: a European consensus statement (2015)

Consensus was achieved on the majority of domains of SNS/SNM for FI and constipation. This should serve as a benchmark for safe and quality practice of SNS/SNM in Europe.
The5ESSSwitching System: Hardware Design (1985)

This paper gives a general description of the 5ESS™ switch hardware design. A significant amount of new hardware, including numerous very-large-scale integration circuits, microprocessors, signal processors, and memory devices, is used in the 5ESS switch design to accomplish its operational and maintenance design objectives. By adhering to the distributed and modular architecture in all the hardware designs, the incorporation of advancing technologies and new functionality is accomplished with ease.
L p Centroidal Voronoi Tessellation and its applications (2010)

This paper introduces L p -Centroidal Voronoi Tessellation ( L p -CVT), a generalization of CVT that minimizes a higher-order moment of the coordinates on the Voronoi cells. This generalization allows for aligning the axes of the Voronoi cells with a predefined background tensor field (anisotropy). L p -CVT is computed by a quasi-Newton optimization framework, based on closed-form derivations of the objective function and its gradient. The derivations are given for both surface meshing (Ω is a triangulated mesh with per-facet anisotropy) and volume meshing (Ω is the interior of a closed triangulated mesh with a 3D anisotropy field). Applications to anisotropic, quad-dominant surface remeshing and to hexdominant volume meshing are presented. Unlike previous work, L p -CVT captures sharp features and intersections without requiring any pre-tagging.
Dynamic Fine-Grained Localization in Ad-Hoc Wireless Sensor Networks (2001)

The recent advances in radio and embedded system technologies have enabled the proliferation of wireless micro-sensor networks. Such wirelessly connected sensors are released in many diverse environments to perform various monitoring tasks. In many such tasks, location awareness is inherently one of the most essential system parameters. It is not only needed to report the origins of events, but also to assist group querying of sensors, routing, and to answer questions on the network coverage. In this paper we present a novel approach to the localization of sensors in an ad-hoc network. We describe a system called AHLoS (Ad-Hoc Localization System) that enables sensor nodes to discover their locations using a set distributed iterative algorithms. The operation of AHLoS is demonstrated with an accuracy of a few centimeters using our prototype testbed while scalability and performance are studied through simulation.
Combined HPLC, NMR Spectroscopy, and Ion-Trap Mass Spectrometry with Application to the Detection and Characterization of Xenobiotic and Endogenous Metabolites in Human Urine (1996)

The direct coupling of HPLC with NMR spectroscopy has been extended by splitting the HPLC eluent after conventional UV detection and sending part to a NMR spectrometer and part to an ion-trap mass spectrometer in a "triplehyphenated" HPLC-NMR-MS system. Combined UV, 1H NMR, and positive-ion electrospray MS detection was achieved in the continuous-flow mode using whole human urine from a subject dosed with acetaminophen. By means of HPLC-NMR-MS, the structural information available from the complementary spectroscopic techniques provided rapid confirmation of the identity of the acetaminophen glucuronide and sulfate metabolites, together with a number of endogenous metabolites. In particular, the HPLC-NMR-MS approach allowed the unequivocal identification of phenylacetylglutamine in human urine, an endogenous metabolite not previously observed in 1H NMR spectra of urine because of extensive overlap with resonances from other metabolites. The analytical advantages and complementarity of NMR and MS techniques in direct hyphenation with HPLC are discussed. The new technique of HPLC-NMR-MS will provide the scope for more comprehensive and fully automated analysis of biofluids and other complex mixtures than was previously available from single hyphenation of these instruments.
Internet implications of telephone access (1999)

What good is a network if it can't be accessed? In fact, access is often a single point of failure for data or telecommunications network subscribers. In the US, the Public Switched Telephone Network is the primary provider for the vast majority of voice service users. The PSTN is also the principal source of circuit-switched access to ISPs for an increasing number of PCs. Therefore, PSTN access availability is a benchmark for those who are considering providing or acquiring Internet access or voice services. The article presents a typical Internet user connection through the local PSTN to an ISP. In the PSTN, local or central office switches serve as network access nodes. Although considerable redundancy and fault tolerance are engineered into both the network itself and these switches, total service outages do occur. In the future, terms such as local carrier and long-distance carrier are likely to disappear as more end-to-end carriers emerge, providing integrated voice and data services. User demands for carrier transparency will dictate having a highly reliable, available, and survivable network access infrastructure. To date, access networks have emphasized connection speed to alleviate end-to-end latency problems. However, as subscribers experience low latency, service providers who offer dependable access will be the survivors. Fast access that isn't reliable won't be acceptable for home e-commerce.
Acoustic Wave Microsensor Arrays for Vapor Sensing (2000)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcoustic Wave Microsensor Arrays for Vapor SensingJay W. GrateView Author Information Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352 Cite this: Chem. Rev. 2000, 100, 7, 2627–2648Publication Date (Web):March 28, 2000Publication History Received24 August 1999Published online28 March 2000Published inissue 1 July 2000https://pubs.acs.org/doi/10.1021/cr980094jhttps://doi.org/10.1021/cr980094jresearch-articleACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views2635Altmetric-Citations352LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Coating materials,Materials,Polymers,Sensors,Thin films Get e-Alerts
Resequencing and copy number analysis of the human tyrosine kinase gene family in poorly differentiated gastric cancer (2009)

The tyrosine kinase (TK) family is an important regulator of signaling pathways that control a variety of physiological and pathological conditions, and a substantial proportion of TK genes are genetically altered in cancer. To clarify the somatic mutation profile of TK genes and discover potential targets for gastric cancer (GC) therapy, we undertook a systematic screening of mutations in the kinase domains of all human TK genes (636 exons of 90 genes) in 17 GC cell lines and 52 microdissected primary GCs with poorly differentiated histology. We identified 26 non-synonymous alterations (22 genes in total) that included 11 sequence alterations in cell lines and 15 somatic mutations in primary tumors. Recurrent mutations were found in four genes including a known oncogene (NTRK3), the Src kinase family (LTK and CSK) and a potential Wnt signal activator (ROR2). In addition, we analyzed copy number alterations of all the TK gene loci in the same cohort samples by array-based comparative genomic hybridization analysis and identified 24 high-level amplifications and two homozygous deletions. Both sequence alteration and frequent copy number aberration were detected in two TK genes (HCK and ERBB2), strongly suggesting that they encode potential oncogenes in GC. Our focused and integrated analyses of systemic resequencing and gene copy number have revealed the novel onco-kinome profile of GC and pave the way to a comprehensive understanding of the GC genome.
Length of the antibody heavy chain complementarity determining region 3 as a specificity‐determining factor (2004)

The antigen binding site of an antibody is made up of residues residing in six hypervariable loops of the heavy and light chains. In most cases several or all of these loops are required for the establishment of the antigen-binding surface. Five of these loops display a limited diversity in length and sequence while the third complementarity determining region (CDR) of the heavy chain is highly different between antibodies not only with respect to sequence but also with respect to length. Its extensive diversity is a key component in the establishment of binding sites allowing for the recognition of essentially any antigen by humoral immunity. The relative importance of its sequence vs its length diversity in this context is however, not very well established. To investigate this matter further we have used an approach employing combinatorial antibody libraries and antigen-specific selection in the search for CDRH3 length and sequence diversity compatible with a given antigen specificity, the major antigenic determinant on the tumour-associated antigen mucin-1. In this way we have now defined heavy chain CDR3 length as a critical parameter in the creation of an antigen-specific binding site. We also propose that this may reflect a dependence of a particular structure of this hypervariable loop, the major carrier of diversity in the binding site, for establishment of a given specificity.
ASYMMETRIC CELL DIVISION IN C. ELEGANS: Cortical Polarity and Spindle Positioning (2004)

The one-cell Caenorhabditis elegans embryo divides asymmetrically into a larger and smaller blastomere, each with a different fate. How does such asymmetry arise? The sperm-supplied centrosome establishes an axis of polarity in the embryo that is transduced into the establishment of anterior and posterior cortical domains. These cortical domains define the polarity of the embryo, acting upstream of the PAR proteins. The PAR proteins, in turn, determine the subsequent segregation of fate determinants and the plane of cell division. We address how cortical asymmetry could be established, relying on data from C. elegans and other polarized cells, as well as from applicable models. We discuss how cortical polarity influences spindle position to accomplish an asymmetric division, presenting the current models of spindle orientation and anaphase spindle displacement. We focus on asymmetric cell division as a function of the actin and microtubule cytoskeletons, emphasizing the cell biology of polarity.
Establishment and Maintenance of Genomic Methylation Patterns in Mouse Embryonic Stem Cells by Dnmt3a and Dnmt3b (2003)

We have previously shown that the DNA methyltransferases Dnmt3a and Dnmt3b carry out de novo methylation of the mouse genome during early postimplantation development and of maternally imprinted genes in the oocyte. In the present study, we demonstrate that Dnmt3a and Dnmt3b are also essential for the stable inheritance, or "maintenance," of DNA methylation patterns. Inactivation of both Dnmt3a and Dnmt3b in embryonic stem (ES) cells results in progressive loss of methylation in various repeats and single-copy genes. Interestingly, introduction of the Dnmt3a, Dnmt3a2, and Dnmt3b1 isoforms back into highly demethylated mutant ES cells restores genomic methylation patterns; these isoforms appear to have both common and distinct DNA targets, but they all fail to restore the maternal methylation imprints. In contrast, overexpression of Dnmt1 and Dnmt3b3 failed to restore DNA methylation patterns due to their inability to catalyze de novo methylation in vivo. We also show that hypermethylation of genomic DNA by Dnmt3a and Dnmt3b is necessary for ES cells to form teratomas in nude mice. These results indicate that genomic methylation patterns are determined partly through differential expression of different Dnmt3a and Dnmt3b isoforms.
Interleukin-8 Released into the Cerebrospinal Fluid after Brain Injury is Associated with Blood–Brain Barrier Dysfunction and Nerve Growth Factor Production (1997)

Interleukin (IL) 8 was measured in CSF of 14 patients with severe traumatic brain injury. IL-8 levels were significantly higher in CSF (up to 8,000 pg/ml) than serum (up to 2,400 pg/ml) (p < 0.05), suggesting intrathecal production. Maximal IL-8 values in CSF correlated with a severe dysfunction of the blood-brain barrier. Nerve growth factor (NGF) was detected in CSF of 7 of 14 patients (range of maximal NGF: 62-12,130 pg/ml). IL-8 concentrations were significantly higher in these patients than in those without NGF (p < 0.01). CSF containing high IL-8 (3,800-7,900 pg/ml) induced greater NGF production in cultured astrocytes (202-434 pg/ml) than samples with low IL-8 (600-1,000 pg/ml), which showed a smaller NGF increase (0-165 pg/ml). Anti-IL-8 antibodies strongly reduced (52-100%) the release of NGF in the group of high IL-8, whereas in the group with low IL-8, this effect was lower (0-52%). The inability of anti-IL-8 antibodies to inhibit the synthesis of NGF completely may depend on cytokines like tumor necrosis factor alpha and IL-6 found in these CSF samples, which may act in association with IL-8. Thus, IL-8 may represent a pivotal cytokine in the pathology of brain injury.
The social impact of a systematic floor cleaner (2012)

Mint is an automatic cleaning robot that sweeps and mops hard-surface floors using dusting and mopping cloths. Thanks to the Northstar navigation technology it systematically cleans and navigates in people's homes. Since it first became commercially available in mid 2010, hundreds of thousands of Mint cleaners are nowadays in use at home. In this paper we investigate the product's social impact with respect to the attitude of customers towards a systematic floor cleaner and how such a robot influences their lifestyle. We first report feedback from users owning the product, and demonstrate how Mint changed their everyday life at home. We then evaluate the results of a survey launched in 2012 that addresses the technical understanding of the product and what impact it has on the social life of their users. Our findings suggest that Mint, on average, saves more than one hour of people's time per week, floors are cleaner leading to healthier homes and lives, systematic cleaning is seen as an important feature, and modifications to the environment to support the navigation of the robot are largely accepted.
Detection and Analysis of Low‐Abundance Cell‐Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets (2009)

Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
Organization and function of the mcrBC genes of Escherichia coli K‐12 (1992)

Many natural DNA sequences are restricted in Escherichia coli K-12, not only by the classic Type I restriction system EcoK, but also by one of three modification-specific restriction systems found in K-12. The McrBC system is the best studied of these. We infer from the base composition of the mcrBC genes that they were imported from an evolutionarily distant source. The genes are located in a hypervariable cluster of restriction genes that may play a significant role in generation of species identity in enteric bacteria. Restriction activity requires the products of two genes for activity both in vivo and in vitro. The mcrB gene elaborates two protein products, only one of which is required for activity in vitro, but both of which contain a conserved amino acid sequence motif identified as a possible GTP-binding site. The mcrC gene product contains a leucine heptad repeat that could play a role in protein-protein interactions. McrBC activity in vivo and in vitro depends on the presence of modified cytosine in a specific sequence context; three different modifications are recognized. The in vitro activity of this novel multi-subunit restriction enzyme displays an absolute requirement for GTP as a cofactor.
Data abstraction, views and updates in RIGEL (1979)

Language constructs to support the development of data base applications provided in the programming language rigel are described. First, the language type system includes relations, views, and tuples as built-in types. Tuple-values are introduced to provide more flexibility in writing procedures that update relations and views.Second, an expression that produces sequences of values, called a generator, is defined which integrates relational query expressions with other iteration constructs found in general-purpose programming languages. As a result, relational expressions can be used in new contexts (e.g., as parameters to procedures) to provide new capabilities (e.g., programmer-defined aggregate functions).Lastly, a data abstraction facility, unlike those proposed for other data base programming languages, is described. It provides a better notation to specify the interface between a program and a data base and to support the disciplined use of views.All of these constructs are integrated into a sophisticated programming environment to enhance the development of well-structured programs.
Methylene blue based protein solder for vascular anastomoses: An in vitro burst pressure study (2000)

PSA with MB solder is able to form high-quality end-to-end anastomoses, with immediate burst pressure profiles similar to those previously described for sutured [Quigley et al., Microsurgery 1985;6:229], lasered [Quigley et al., Microsurgery 1985;6:229], and soldered anastomoses [Small et al., J Clin Laser Med Surg 1997;15:205]. The relationship between burst pressure strength and chromophore absorption is discussed.
Guaranteeing High Availability to Client-Server Communications (2008)

One of the Next Generation Internet (NGI) objectives is to offer services supported by large bandwidth connectivity, such as multimedia services. However their Quality of Service (QoS) is strongly influenced also by resilience. This paper focuses on the important problem of server selection with the highest two-terminal availability for distributed multimedia services. An analytical model gives optimal global server selection in terms of the highest two-terminal reliability for client-server connection in simple networks. For more complex networks a heuristic algorithm is utilized. The proposed algorithm allows to obtain better reliability values than the common shortest path server selection scheme.