gebhart/patrepeval huggingface dataset:
A sequential dimerization mechanism for erythropoietin receptor activation. (1996)

We have probed the interaction of human erythropoietin (EPO) with its receptor (EPO-R) by analyzing a panel of 17 EPO mutants in a variety of in vitro assays. Mutant proteins were expressed in 293s cells and quantified by using an N-terminal epitope tag in conjunction with a surface plasmon resonance assay. Receptor binding was studied using both a soluble form of the EPO-R extracellular domain in an ELISA-format binding competition assay and full-length EPO-R in transfected BaF3 cells. Proliferative activity of the mutants was also determined in the BaF3-derived cell line and was correlated with the results from binding assays. Based on the results of these assays, we identified two distinct receptor binding sites on the EPO molecule. We propose that one site, containing residues Arg-150 and Lys-152, binds initially to EPO receptor on the cell surface. A second site, containing Arg-103 and Ser-104 (and possibly Arg-14), is involved in binding a second EPO-R at the cell surface, thus forming a homodimeric receptor complex. Furthermore, we demonstrate that one EPO mutant (R103A), which has previously been shown to lack proliferative function, is in fact an EPO antagonist. Taken together, these data support a sequential dimerization mechanism of EPO-R activation.
Photopatterning of Self-Assembled Alkanethiolate Monolayers on Gold: A Simple Monolayer Photoresist Utilizing Aqueous Chemistry (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotopatterning of Self-Assembled Alkanethiolate Monolayers on Gold: A Simple Monolayer Photoresist Utilizing Aqueous ChemistryJingyu Huang, David A. Dahlgren, and John C. HemmingerCite this: Langmuir 1994, 10, 3, 626–628Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March 1994https://pubs.acs.org/doi/10.1021/la00015a005https://doi.org/10.1021/la00015a005research-articleACS PublicationsRequest reuse permissionsArticle Views816Altmetric-Citations211LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Predictive value of mid luteal progesterone concentration before luteal support in controlled ovarian hyperstimulation with intrauterine insemination (2004)

Increased mid luteal serum P4 levels are not associated with a higher CPR in women undergoing COH with IUI. However, a low mid luteal P4 level < or =25 nmol/L may help predict treatment failure.
Triplet Energy Back Transfer in Conjugated Polymers with Pendant Phosphorescent Iridium Complexes (2006)

The nature of Dexter triplet energy transfer between bonded systems of a red phosphorescent iridium complex 13 and a conjugated polymer, polyfluorene, has been investigated in electrophosphorescent organic light-emitting diodes. Red-emitting phosphorescent iridium complexes based on the [Ir(btp)(2)(acac)] fragment (where btp is 2-(2'-benzo[b]thienyl)pyridinato and acac is acetylacetonate) have been attached either directly (spacerless) or through a -(CH(2))(8)- chain (octamethylene-tethered) at the 9-position of a 9-octylfluorene host. The resulting dibromo-functionalized spacerless (8) or octamethylene-tethered (12) fluorene monomers were chain extended by Suzuki polycondensations using the bis(boronate)-terminated fluorene macromonomers 16 in the presence of end-capping chlorobenzene solvent to produce the statistical spacerless (17) and octamethylene-tethered (18) copolymers containing an even dispersion of the pendant phosphorescent fragments. The spacerless monomer 12 adopts a face-to-face conformation with a separation of only 3.6 A between the iridium complex and fluorenyl group, as shown by X-ray analysis of a single crystal, and this facilitates intramolecular triplet energy transfer in the spacerless copolymers 17. The photo- and electroluminescence efficiencies of the octamethylene-tethered copolymers 18 are double those of the spacerless copolymers 17, and this is consistent with suppression of the back transfer of triplets from the red phosphorescent iridium complex to the polyfluorene backbone in 18. The incorporation of a -(CH(2))(8)- chain between the polymer host and phosphorescent guest is thus an important design principle for achieving higher efficiencies in those electrophosphorescent organic light-emitting diodes for which the triplet energy levels of the host and guest are similar.
Monoclonal antibodies against porcine LFA-1: Species cross-reactivity and functional effects of β-subunit-specific antibodies (1989)

As a first step in developing a porcine model system to study the effect of LFA-1-specific monoclonal antibodies on allograft rejection, we have identified anti-human LFA-1 monoclonal antibodies that cross-react with porcine leukocytes and have used one of these antibodies against the LFA-1 beta-subunit to purify porcine LFA-1. Immunization of Balb/c mice with the purified antigen yielded eight monoclonal antibodies directed against the beta-subunit of porcine LFA-1. These antibodies immunoprecipitated pig proteins corresponding to the human alpha-subunits of LFA-1 (CD11a), Mac-1 (CD11b) and LeuM5 (CD11c), and their common beta-subunit (CD18). Under both reducing and non-reducing conditions, the porcine beta-subunit showed a different electrophoretic mobility from that of the human beta-subunit. In addition, results of peptide mapping studies revealed structural differences between the beta-subunits of human and porcine LFA-1. The anti-LFA-1 beta-chain antibodies were found to cross-react with antigens expressed on rat, mouse, hamster, rabbit, dog, cow and human leukocytes, indicating that the beta-subunit contains highly conserved epitopes. Competitive binding studies showed that these antibodies and three anti-human LFA-1 antibodies defined at least four distinct epitopes on the porcine beta-subunit, three of which were also found on human beta-subunits. Whereas the cross-reactive anti-human beta-subunit antibody H52 profoundly inhibited mitogenic and allogenic stimulation of porcine T lymphocytes as well as cytotoxic T cell and natural killer (NK) cell function, the anti-porcine LFA-1 monoclonal antibodies had very limited effect on porcine lymphocyte function. Our results demonstrate that porcine leukocytes express the LFA-1 molecule and that this antigen is involved in T cell and NK cell functions, as has previously been shown in human and murine systems. We conclude that the pig may serve as a useful model in which to test the effect of anti-LFA-1 antibodies on allograft rejection.
Nonunion of the Humerus Following Intramedullary Nailing Treated by Ilizarov Hybrid Fixation (1998)

A case of a posttraumatic humeral shaft nonunion, after intramedullary stabilization with a Seidel nail, is presented. Severe osteoporosis, an oligotrophic nonunion, subclinical infection, and adhesive capsulitis of the glenohumeral joint were present. Due to the subclinical infection and severe osteoporosis, other major invasive therapeutic options such as intramedullary nailing or compression plating and bone grafting were not applicable. Nonoperative treatment was also not indicated secondary to the pain and disability present. External fixation with the Ilizarov hybrid fixator seemed to offer a minimally invasive treatment modality without the need of additional bone grafting. After fourteen weeks of "callus massage," consisting of closed alternating compression and distraction with an Ilizarov hybrid fixator, osseous consolidation was achieved. Eight months after Ilizarov treatment the patient had returned to work as a mechanic. At the one-year follow-up examination, the patient presented pain free and with near normal shoulder and elbow motion, with stable osseous consolidation of the humerus. In some cases of nonunion of the humerus shaft, when standard treatment options are not recommended, external fixation with an Ilizarov hybrid fixator may offer a salvage procedure with a successful clinical outcome.
Long term in vitro biostability of segmented polyisobutylene‐based thermoplastic polyurethanes (2010)

Long term in vitro biostability of thermoplastic polyurethanes (TPUs) containing mixed polyisobutylene (PIB)/poly(tetramethylene oxide) (PTMO) soft segment was studied under accelerated conditions in 20% H(2)O(2) solution containing 0.1M CoCl(2) at 50 °C to predict resistance to metal ion oxidative degradation (MIO) in vivo. The PIB-based TPUs showed significant oxidative stability as compared to the commercial controls Pellethane 2363-55D and 2363-80A. After 12 weeks in vitro the PIB-PTMO TPUs with 10-20% PTMO in the soft segment showed 6-10% weight loss whereas the Pellethane TPUs degraded completely in about 9 weeks. Attenuated total reflectance Fourier transform infrared spectroscopy confirmed the degradation of Pellethane samples via MIO by the loss of the ∼1110 cm(-1) aliphatic C-O-C stretching peak height attributed to chain scission, and the appearance of a new peak at ∼1174 cm(-1) attributed to crosslinking. No such changes were apparent in the spectra of the PIB-based TPUs. The PIB-based TPUs exhibited 10-30% drop in tensile strength compared to 100% for the Pellethane TPUs after 12 weeks. The molecular weight of the PIB-based TPUs decreased slightly (10-15%) at 12 weeks. The Pellethane TPUs showed a dramatic decrease in M(n) and an increase in low molecular weight degradation product. Scanning electron microscopy (SEM) showed severe cracking in the Pellethane samples after 2 weeks, whereas the PIB-based TPUs exhibited a continuous surface morphology. The weight loss, tensile, and SEM data correlate well with each other and indicate excellent biostability of these materials.
RFI suppression for ultra wideband radar (1997)

An estimate-and-subtract algorithm is presented for the real-time digital suppression of radio frequency interference (RFI) in ultrawideband (UWB) synthetic aperture radar (SAR) systems used for foliage- and ground-penetrating imaging. The algorithm separately processes fixed- and variable-frequency interferers. Excision of estimated targets greatly reduces bias in RFI estimates, thereby reducing target energy loss and sidelobe levels in SAR imagery. Performance is demonstrated on data collected with the Army Research Laboratory's UWB rail SAR.
Metalenses at visible wavelengths: past, present, perspectives (2017)

Abstract The so‐called ‘flat optics’ that shape the amplitude and phase of light with high spatial resolution are presently receiving considerable attention. Numerous journal publications seemingly offer hope for great promises for ultra‐flat metalenses with high efficiency, high numerical aperture, broadband operation… We temperate the expectation by referring to the current status of metalenses against their historical background, assessing the technical and scientific challenges recently solved and critically identifying those that still stand in the way. image
Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway. (1994)

The wild-type p53 gene product plays an important role in the control of cell proliferation, differentiation, and survival. Altered function is frequently associated with changes in p53 stability. We have studied the role of the ubiquitination pathway in the degradation of p53, utilizing a temperature-sensitive mutant, ts20, derived from the mouse cell line BALB/c 3T3. We found that wild-type p53 accumulates markedly because of decreased breakdown when cells are shifted to the restrictive temperature. Introduction of sequences encoding the human ubiquitin-activating enzyme E1 corrects the temperature sensitivity defect in ts20 and prevents accumulation of p53. The data therefore strongly indicate that wild-type p53 is degraded intracellularly by the ubiquitin-mediated proteolytic pathway.
A Raf-1-related p110 polypeptide associates with the CD4-p56lck complex in T cells. (1992)

The CD4 and CD8 antigens on T cells have been shown to associate with the Src family member p56lck and a GTP-binding protein, p32. The identification of receptor interactions with intracellular mediators is essential in the elucidation of downstream signals mediated by engagement of these receptor complexes. In this study, we report the detection of an additional 110-kDa polypeptide (p110) associated with the CD4-p56lck complex in human peripheral blood T lymphocytes and leukemic T-cell lines. p110 bound preferentially to CD4-p56lck as an assembled complex and poorly, if at all, to the individual components. p110 was recognized directly by an antiserum to the C-terminal region of the serine/threonine kinase Raf-1 and is related to a p110 polypeptide detected in anti-Raf-1 immunoprecipitates. Despite its association with the CD4-p56lck complex, p110 was found to be phosphorylated predominantly on serine residues. Furthermore, phorbol ester treatment of cells resulted in a transient increase in the detection of p110 associated with CD4-p56lck, concomitant with the modulation of CD4-p56lck from the cell surface. This Raf-1-related p110 is therefore likely to play a role in signals generated from the CD4-p56lck complex. p110 may serve as a bridge between the CD4-p56lck complex and the serine/threonine kinase pathways of T-cell activation.
Yeast Artificial Chromosomes (2012)

Abstract Yeast artificial chromosomes (YACs) are shuttle‐vectors that can be amplified in bacteria and employed for the cloning and manipulation of large deoxyribonucleic acid (DNA) inserts (up to 3 Mb pairs) in the yeast Saccharomyces cerevisiae . Artificial chromosomes can be conveniently built and modified in yeast cells using in vivo homologous recombination, a novel process known as ‘recombineering’. The capacity of YACs to accommodate large DNA fragments is exploited to clone clusters of genes surrounded by their native DNA context, where regulatory elements are located. This is important for biotechnology, when YACs are used for engineering genetic determinants of new biochemical pathways for production of secondary metabolites and for heterologous protein expression. YACs can be retrofitted with the appropriate selectable markers and transmitted to cells of different organisms allowing the generation of transgenic animals. Finally, YACs are largely employed in the production of full‐scale genomic libraries, for mapping and functional analysis purposes. Key Concepts: Yeast artificial chromosomes (YACs) represent the top instruments for the study of eukaryotic genomes and for mobilisation of large genetic elements among bacteria and eukaryotes. YACs behave like naturally existing chromosomes, provided that they are of the proper size, showing comparable stability. YACs can be manipulated directly by classical genetic engineering as well as by modern recombineering technology. Properly retrofitted, YACs can be used in many different organisms, for cloning or genome analysis. Chromosomal translocation can be studied using disposable YACs that do not harbour genetic information essential for cell function.
Cancer Gene Therapy by Direct Tumor Injections of a Nonviral T7 Vector Encoding a Thymidine Kinase Gene (1998)

Previously, we described a nonviral cytoplasmic gene therapy vector system based on the T7 autogene concept. This system has been shown to achieve rapid and high levels of gene expression in a variety of animal cells and tissues. To test the utility of the system in vivo tumor ablation, a T7 cancer gene therapy plasmid vector, pT7T7/T7TK, was constructed. This nonviral vector contains a T7 autogene, T7T7, and a human herpes simplex virus thymidine kinase (HSV-TK) gene driven by a second T7 promoter (T7TK). When co-transfected with T7 RNA polymerase (T7 RNAP) into cultured human osteosarcoma 143B cells, abut 10-20% of the cells were found to express HSV-TK, and more than 90% of the cells were killed in the presence of 1 microM ganciclovir (GCV) within 4 days after DNA transfection. The increase in killing above the transfection frequency is due to a "bystander" effect among transfected and untransfected 143B cells. Direct injections of pT7T7/T7TK into 143B tumors grown in nude mice resulted in TK gene expression in tumor cells located near the injection sites as revealed by the immunohistochemical staining. Repeated tumor injections of the pT7T7/T7TK vector and intraperitoneal (i.p.) injections of GCV resulted in inhibition of tumor growth and in tumor shrinkage in 6 out of 10 treated nude mice. Three of those six tumors fully regressed shortly after the end of the GCV injections. All of the full tumor regressions were found to be permanent and no apparent tumor relapses were observed for the rest of the lives of the treated nude mice after the initial tumor ablations. These results, combined with the nonviral and rapid cytoplasmic gene expression features, suggest that the T7 vector may be a good candidate for cancer gene therapy and other medical and biological applications.
Effect of potent redox-modulating manganese porphyrin, MnTM-2-PyP, on the Na+/H+exchangers NHE-1 and NHE-3 in the diabetic rat (2009)

Increased expression of Na(+)/H(+) exchanger (NHE) and Na(+),K(+)-ATPase activity have been demonstrated in diabetic nephropathy and are implicated in the development of hypertension. The aim of this study was to investigate the effect of a synthetic manganese porphyrin SOD mimic and peroxynitrite scavenger, Mn(III) 5,10,15,20-tetrakis(N-methylpyridinium-2-yl)porphyrin (MnTM-2-PyP) on the expression of NHE and Na(+),K(+)-ATPase activity in the kidneys of streptozotocin (STZ) diabetic rats. MnTM-2-PyP administration (1 mg/kg/day) started immediately after STZ and lasted 2 months. Glucose and glycosylated hemoglobin levels were measured in blood. NHE-1 and NHE-3 isoform expression, Na(+),K(+)-ATPase activity, and markers of ROS/RNS-induced damage were determined in kidney homogenates. Diabetes caused lipid peroxidation, inactivation of aconitase, and increase of nitrotyrosine, which paralleled an increase in NHE-1 and NHE-3 expression and Na(+),K(+)-ATPase activity. MnTM-2-PyP treatment had no effect on blood glucose and glycosylated hemoglobin, but suppressed lipid peroxidation and nitrotyrosine, protected aconitase against inactivation, and reversed the induction of NHE-1 and NHE-3 isoforms. Na(+)/H(+) exchanger is under the control of redox-based cellular transcriptional activity, including members of the SP family of transcription factors. Mn(III) alkylpyridylporphyrins were previously found to inhibit activation of major transcription factors, including SP-1 via scavenging of signaling ROS/RNS. Therefore, our data suggest that, by reducing the levels of ROS/RNS, MnTM-2-PyP might interfere with signaling pathways responsible for NHE up-regulation.
Spectral Networks and Locally Connected Networks on Graphs (2013)

Convolutional Neural Networks are extremely efficient architectures in image and audio recognition tasks, thanks to their ability to exploit the local translational invariance of signal classes over their domain. In this paper we consider possible generalizations of CNNs to signals defined on more general domains without the action of a translation group. In particular, we propose two constructions, one based upon a hierarchical clustering of the domain, and another based on the spectrum of the graph Laplacian. We show through experiments that for low-dimensional graphs it is possible to learn convolutional layers with a number of parameters independent of the input size, resulting in efficient deep architectures.
Characterization of the mouse met proto-oncogene. (1988)

The DNA sequence of cDNA clones prepared from transcripts of the mouse met proto-oncogene reveals that the mouse met gene encodes a 1380 amino acid protein with the characteristics of a growth factor receptor. This protein can be divided into several putative domains, including an intracellular protein tyrosine kinase domain, a transmembrane domain and a 929 amino acid extracellular domain, possessing a potential proteolytic cleavage site with the sequence Lys-Arg-Arg-Lys-Arg-Ser. To gain additional insights into the function of the met protein we have examined the level of met transcripts in tissues of the late-gestation mouse conceptus. Transcription of met was observed in most of the tissues analysed, but the highest levels of met mRNA were detected in the yolk sac, amnion and kidney; no transcripts were detectable in the calvaria. Chromosomal localization using a series of mouse-hamster hybrid cell lines has demonstrated that met is located on mouse chromosome 6.
Vitamin D Deficiency (2007)

Once foods in the United States were fortified with vitamin D, rickets appeared to have been conquered, and many considered major health problems from vitamin D deficiency resolved. But vitamin D deficiency is common. This review considers the role of vitamin D in skeletal and nonskeletal health and suggests strategies for the prevention and treatment of vitamin D deficiency.
Therapy for attention-deficit hyperactivity disorder (1994)

Attention-deficit hyperactivity disorder (ADHD) is a common, chronic neurological and behavioral syndrome in children, adolescents, and adults. Research studies carried out with subjects referred to specialists for treatment of ADHD have shown that methylphenidate hydrochloride improves behavior and academic performance in both children and adolescents, at least in the short term. Dextroamphetamine sulfate, pemoline, and desipramine hydrochloride are reasonable second choices for those patients who cannot tolerate or do not respond to methylphenidate. Cognitive-behavioral and family therapies, while intuitively appealing, appear to have weak effects and little empirical validation. The long-term outcome of ADHD as seen in primary care settings is not predictable.
Design and Evaluation of Integrity Algorithms for PPP in Kinematic Applications (2018)

UAV and autonomous platforms can greatly benefit from an assured position solution with high integrity error bounds. The expected high degree of connectivity in these vehicles will allow users to receive real time precise clock and ephemeris corrections, which enable the use of Precise Point Positioning techniques. Up to now, these techniques have mostly been used to provide high accuracy, rather than focusing on high integrity applications. In this paper we apply the methodology and algorithms used in aviation to determine position error bounds with high integrity (or protection levels) for a PPP position solution.