gebhart/patrepeval huggingface dataset:
Urinary Biomarkers of Podocyte Injury (2008)

Podocyturia may be an expression of glomerular disease and is evaluated using urinary podocyte-specific molecules. This article discusses the technical problems of detection and quantification of podocyturia using immunofluorescent staining of specific proteins, urinary podocyte culture, flow cytometry and urinary podocyte-specific molecules (including PCR, immunoblotting and ELISA). Technical problems mean that it is still impossible to define a biomarker for urinary podocyte injury. This article also discusses the future perspectives for podocyturia studies, including fluorescence-activated cell sorting and the study of the proteome of urinary exosomes.
The New Paradigm Wars: Is There Room for Rigorous Practitioner Knowledge in Schools and Universities? (1999)

ccording to Schon (1995) new scholarship implies a kind of action research with norms of its own, which will conflict with the norms of technical rationality-the prevailing epistemology built into the research universities (p. 27). The battle of snails that Schon refers to echoes the wars among positivists, interpretivists, and critical theorists, satirically described by Gage (1989) in the pages of Educational Researcher. While we believe that practitioner research cannot be subsumed under any of Gage's three paradigms without doing it damage, our purpose in this article is not to argue for separate paradigm status. Nevertheless, we believe that the insider status of the researcher, the centrality of action, the requirement of spiraling self-reflection on action, and the intimate, dialectical relationship of research to practice, all make practitioner research alien (and often suspect) to researchers who work out of Gage's three academic paradigms. If anything, academic traditions of feminist and poststructural research might be more compatible with these characteristics. It is interesting to speculate on why metaphors of war and battles are evoked to discuss these epistemological debates. While it could be attributed to the academic version
Algorithms in Java (2003)

PART V. GRAPH ALGORITHMS. 17. Graph Properties and Types. Glossary. Graph ADT. Adjacency-Matrix Representation. Adjacency-Lists Representation. Variations, Extensions, and Costs. Graph Generators. Simple, Euler, and Hamilton Paths. Graph-Processing Problems. 18. Graph Search. Exploring a Maze. Depth-First Search. Graph-Search ADT Functions. Properties of DFS Forests. DFS Algorithms. Separability and Biconnectivity. Breadth-First Search. Generalized Graph Search. Analysis of Graph Algorithms. 19. Digraphs and DAGs. Glossary and Rules of the Game. Anatomy of DFS in Digraphs. Reachability and Transitive Closure. Equivalence Relations and Partial Orders. DAGs. Topological Sorting. Reachability in DAGs. Strong Components in Digraphs. Transitive Closure Revisited. Perspective. 20. Minimum Spanning Trees. Representations. Underlying Principles of MST Algorithms. Prim's Algorithm and Priority-First Search. Kruskal's Algorithm. Boruvka's Algorithm. Comparisons and Improvements. Euclidean MST. 21. Shortest Paths. Underlying Principles. Dijkstra's Algorithm. All-Pairs Shortest Paths. Shortest Paths in Acyclic Networks. Euclidean Networks. Reduction. Negative Weights. Perspective. 22. Network Flow. Flow Networks. Augmenting-Path Maxflow Algorithms. Preflow-Push Maxflow Algorithms. Maxflow Reductions. Mincost Flows. Network Simplex Algorithm. Mincost-Flow Reductions. Perspective. References for Part Five. Index.
Calcium Responses in Fibroblasts from Asymptomatic Members of Alzheimer's Disease Families (1998)

We have previously identified alterations of K+ channel function, IP3-mediated calcium release, and Cp20 (a memory-associated GTP binding protein) in fibroblasts from Alzheimer's disease (AD) patients vs controls. Some of these alterations can be integrated into an index that distinguishes AD patients from controls with both high specificity and high sensitivity. We report here that alterations in IP3-mediated calcium responses are present in a large proportion of AD family members (i.e., individuals at high risk) before clinical symptoms of Alzheimer's disease are present. This was not the case if such members later "escaped" AD symptoms. This preclinical calcium signal correlate of later AD does not reflect, however, the presence of the PS1 familial AD gene.
A strategy for rational design of fully synthetic glycopeptide conjugate vaccines (1997)

The present study describes a strategy to rationally design fully synthetic glycopeptide conjugate vaccines. Glycopeptide immunogens were constructed by coupling synthetic oligosaccharides comprising repeating units of synthetic 3-beta-D-ribose-(1-1)-D-ribitol-5-phosphate (sPRP) to synthetic peptides containing potent T-helper cell determinants and B-cell epitopes of the Haemophilus influenzae type b (Hib) outer membrane proteins (OMPs) P1, P2, and P6. Rabbit immunogenicity studies revealed that some of these fully synthetic glycoconjugates were capable of eliciting high titers of both anti-PRP and anti-OMP immunoglobulin G antibodies. In addition, we systematically investigated the factors which could influence their immunogenicity. We observed that the magnitude of the anti-PRP antibody response markedly depended on the relative spatial orientation of sPRP and T-cell epitopes, the anti-PRP antibody response was enhanced when a multiple antigenic peptide was used as a carrier, the anti-PRP antibody response was optimal for three PRP repeating units, and lipidation of peptide-PRP conjugates had a minimal effect on the magnitude of the anti-PRP antibody response. The results of this study clearly demonstrate that coupling a carbohydrate hapten to a peptide can provide T-cell help and convert it into a T-cell-dependent antigen. The antisera raised against these conjugates were also found to be protective against Hib infection in the infant rat model of bacteremia.
Preparing a Poster Presentation (2016)

At some time or other, you may find yourself having to prepare a poster presentation. Posters are an excellent way of conveying a lot of information in a concise, efficient, and compelling form. They are especially useful for early-career scientists – including psychological scientists – to learn how to communicate effectively and also to meet colleagues from other institutions.
Failure of growth hormone to alter the biomechanics of fracture-healing in a rabbit model. (1992)

Standardized tibial osteotomies were created and stabilized with external fixation in twenty-seven skeletally mature rabbits. Fourteen animals were treated with a daily injection of human growth hormone (150 micrograms per kilogram of body weight), and thirteen received a daily injection of saline solution. Serial non-destructive biomechanical tests, radiography, and determinations of the levels of serum insulin-like growth-factor I were performed for all of the animals. Destructive strength-testing of the sites of osteotomy was performed at four, six, or eight weeks. Twenty-five of the twenty-seven osteotomies healed uneventfully. There were no significant differences in the serial biomechanical measurements at the sites of the healing osteotomies, in the radiographic measurements, or in the ultimate strength of the sites of the osteotomy between the two groups. The mean level of serum insulin-like growth-factor I increased 33 per cent relative to the preoperative baseline level in the group that received growth hormone and increased 10 per cent in the control group. This difference was not statistically significant. There was no significant correlation between the biomechanical properties at the sites of the osteotomies and the levels of serum insulin-like growth-factor I. Administration of growth hormone had no measurable effect on fracture-healing in this model of normal animals. It remains to be determined whether injection of growth hormone might affect healing when there is a state of deficiency of endogenous growth hormone or when there is a non-union of a fracture.
Yellow light emission of Vibrio fischeri strain Y-1: purification and characterization of the energy-accepting yellow fluorescent protein. (1987)

A strain of luminous bacteria, Vibrio fischeri Y-1, emits yellow light rather than the blue-green emission typical of other luminous bacteria. The yellow emission has been postulated previously to result from energy transfer from an electronically excited species formed in the bacterial luciferase-catalyzed reaction to a secondary emitter protein, termed the yellow fluorescent protein (YFP). We report here the purification of YFP to homogeneity without loss of the chromophore. The protein was found to be a homodimer of Mr 22,000 subunits with one weakly bound FMN per subunit. The FMN-protein complex was stabilized by 10% (vol/vol) glycerol in the buffers, allowing purification of the active holo-YFP. The protein migrated as a single spot with an isoelectric point of approximately 6.5 on two-dimensional polyacrylamide gel electrophoresis and gave an N-terminal sequence of Met-Phe-Lys-Gly-Ile-Val-Glu-Gly-Ile-Gly-Ile-Ile-Glu-Lys-Ile. Addition of purified YFP to a reaction in which luciferase was supplied with FMNH2 (reduced FMN) by a NADH:FMN oxidoreductase resulted in a dramatic enhancement in the intensity of bioluminescence and an additional peak in the emission spectrum at about 534 nm. The resulting bimodal bioluminescence emission spectrum had peaks at 484 nm, apparently due to emission from the luciferase-flavin complex, and at 534 nm, corresponding to the fluorescence emission maximum of YFP. This bimodal spectrum closely matched the emission spectrum in vivo.
Utility of bipolar electrocautery scissors for cervical conization. (2002)

Bipolar electrocautery scissors were safe and useful in cervical conization by reducing the operating time and blood loss without increasing postoperative morbidity.
Recombinant human interleukin 1 alpha: purification and biological characterization. (1986)

Interleukin 1 (IL 1) is a polypeptide hormone produced by activated macrophages that affects many different cell types involved in immune and inflammatory responses. The cloning and expression of a murine IL 1 cDNA in Escherichia coli encoding a polypeptide precursor of 270 amino acids has been reported, and expression of the carboxy-terminal 156 amino acids of this precursor in E. coli yields biologically active IL 1. By using the murine IL 1 cDNA as a probe, we have isolated its human homolog from cDNA generated to lipopolysaccharide-stimulated human leukocyte mRNA. Nucleotide sequence analysis of this cDNA predicts a protein of analysis of this cDNA predicts a protein of 271 amino acids (termed IL 1 alpha) which shows congruent to 61% homology to its murine counterpart but only 27% homology to a recently characterized human IL 1 precursor (IL 1 beta). We have expressed the carboxy-terminal 154 amino acids of IL 1 alpha in E. coli, purified this protein to homogeneity, and have compared it with pure recombinant murine IL 1 in several different IL 1 assays based on murine and human cells. Recombinant IL 1 is capable of stimulating T cell and fibroblast proliferation and inducing fibroblast collagenase and prostaglandin production, thus proving that a single molecule has many of the activities previously ascribed to only partially purified IL 1 preparations. Our results indicate that there exists a family of at least two human IL 1 genes (alpha and beta) whose dissimilar protein products have similar biological activities.
Generation of Perspective and Panoramic Video from Omnidirectional Video (1997)

Existing software systems for visual exploration are limited in their capabilities in that they are only applicable to static omnidirectional images. We present a software system that has the capability to generate at video rate (30 Hz), a large number of perspective and panoramic video streams from a single omnidirectional video input, using no more than a PC. This permits a remote user to create multiple perspective and panoramic views of a dynamic scene, where the parameters of each view (viewing direction, field of view, and magnification) are controlled via an interactive device such as a mouse, joystick or a head-tracker. 1 Introduction Remote visual exploration systems such as QuickTime VR [Chen-1995] allow a user to navigate around a visual environment. This is done by simulating a virtual camera whose parameters are controlled by the user. A fundamental limitation of existing systems is that they are restricted to static environments, i.e. a single wide-angle image of a sce...
New Fluorescent Substrate Enables Quantitative and High-Throughput Examination of Vesicular Monoamine Transporter 2 (VMAT2) (2013)

Vesicular monoamine transporter 2 (VMAT2) is an essential component of the monoaminergic neurotransmission system in the brain as it transports monoamine neurotransmitters from the neuronal cytosol into the synaptic vesicles and thus contributes to modulation of neurotransmitter release. Considering the continuing interest in VMAT2 as a drug target, as well as a target for the design of imaging probes, we have developed a fluorescent substrate well suited for the study of VMAT2 in cell culture. Herein, we report the synthesis and characterization of a new fluorescent probe, FFN206, as an excellent VMAT2 substrate capable of detecting VMAT2 activity in intact cells using fluorescence microscopy, with subcellular localization to VMAT2-expressing acidic compartments without apparent labeling of other organelles. VMAT2 activity can also be measured via microplate reader. The apparent Km of FFN206 at VMAT2 was found to be 1.16 ± 0.10 μM, similar to that of dopamine. We further report the development and validation of a cell-based fluorescence assay amenable to high-throughput screening (HTS) using VMAT2-transfected HEK cells (Z'-factor of 0.7-0.8), enabling rapid identification of VMAT2 inhibitors and measurement of their inhibition constants over a broad range of affinities. FFN206 thus represents a new tool for optical examination of VMAT2 function in cell culture.
Joint-layer encoder optimization for HEVC scalable extensions (2014)

Scalable video coding provides an efficient solution to support video playback on heterogeneous devices with various channel conditions in heterogeneous networks. SHVC is the latest scalable video coding standard based on the HEVC standard. To improve enhancement layer coding efficiency, inter-layer prediction including texture and motion information generated from the base layer is used for enhancement layer coding. However, the overall performance of the SHVC reference encoder is not fully optimized because rate-distortion optimization (RDO) processes in the base and enhancement layers are independently considered. It is difficult to directly extend the existing joint-layer optimization methods to SHVC due to the complicated coding tree block splitting decisions and in-loop filtering process (e.g., deblocking and sample adaptive offset (SAO) filtering) in HEVC. To solve those problems, a joint-layer optimization method is proposed by adjusting the quantization parameter (QP) to optimally allocate the bit resource between layers. Furthermore, to make more proper resource allocation, the proposed method also considers the viewing probability of base and enhancement layers according to packet loss rate. Based on the viewing probability, a novel joint-layer RD cost function is proposed for joint-layer RDO encoding. The QP values of those coding tree units (CTUs) belonging to lower layers referenced by higher layers are decreased accordingly, and the QP values of those remaining CTUs are increased to keep total bits unchanged. Finally the QP values with minimal joint-layer RD cost are selected to match the viewing probability. The proposed method was applied to the third temporal level (TL-3) pictures in the Random Access configuration. Simulation results demonstrate that the proposed joint-layer optimization method can improve coding performance by 1.3% for these TL-3 pictures compared to the SHVC reference encoder without joint-layer optimization.
1-Sila-3-metallacyclobutanes, precursors for the generation of highly electrophilic Group 4 metallocene alkyl cations. Spectroscopic and structural evidence of a weakly bound THF ligand in [(C5Me5)2Zr(CH2SiMe3)(THF)][BPh4] (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1-Sila-3-metallacyclobutanes, precursors for the generation of highly electrophilic Group 4 metallocene alkyl cations. Spectroscopic and structural evidence of a weakly bound THF ligand in [(C5Me5)2Zr(CH2SiMe3)(THF)][BPh4]David M. Amorose, Rip A. Lee, and Jeffrey L. PetersenCite this: Organometallics 1991, 10, 7, 2191–2198Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/om00053a023https://doi.org/10.1021/om00053a023research-articleACS PublicationsRequest reuse permissionsArticle Views130Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Bioorganic studies of transmitter receptors with philanthotoxin analogs (1994)

Abstract
DNA relatedness among species of Enterobacter and Serratia (1976)

Species of Enterobacter and Serratia were examined for deoxyribonucleic acid relatedness to Klebsielleae, to atypical erwiniae, and to other members of Enterobacteriaceae. Deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. Strains of Enterobacter cloacae formed two separate hybridization groups that correlate with the presence or absence of yellow pigment. Pigmented E. cloacae were 75-100% related, but they were only 40-50% related to unpigmented strains. Conversely, unpigmented strains were 70% or more related but were only 40-50% related to the pigmented strains. Both pigmented and unpigmented E. cloacae were 40-45% related to Enterobacter aerogenes and klebsiellae, and 20-30% related to Serratia species and Enterobacter hafniae. Atypical erwiniae were highly related to E. cloacae. Serratia marcescens strains formed one closely related group. Serratia liquefaciens strains formed a single, more disperse, relatedness group, as did isolates of Serratia rubidaea. These species were related throughout a substantial portion of their genomes. A group of lysine-positive "Citrobacter-like" strains were 40-50% related to Serratia species. Only four E. hafniae strains were tested. Two of these were highly related, while the other two were only 50% related to the reference strain. Enterobacter hafniae was only 15-20% related to other Enterobacteriaceae.
A Draft Sequence of the Rice Genome ( Oryza sativa L. ssp. indica ) (2002)

The genome of the japonica subspecies of rice, an important cereal and model monocot, was sequenced and assembled by whole-genome shotgun sequencing. The assembled sequence covers 93% of the 420-megabase genome. Gene predictions on the assembled sequence suggest that the genome contains 32,000 to 50,000 genes. Homologs of 98% of the known maize, wheat, and barley proteins are found in rice. Synteny and gene homology between rice and the other cereal genomes are extensive, whereas synteny with Arabidopsis is limited. Assignment of candidate rice orthologs to Arabidopsis genes is possible in many cases. The rice genome sequence provides a foundation for the improvement of cereals, our most important crops.
An IFN-γ-Inducible Transcription Factor, IFN Consensus Sequence Binding Protein (ICSBP), Stimulates IL-12 p40 Expression in Macrophages (2000)

IL-12 is a cytokine that links innate and adaptive immunity. Its subunit p40 is induced in macrophages following IFN-gamma/LPS stimulation. Here we studied the role for IFN consensus sequence binding protein (ICSBP), an IFN-gamma/LPS-inducible transcription factor of the IFN regulatory factor (IRF) family in IL-12 p40 transcription. Macrophage-like cells established from ICSBP-/- mice did not induce IL-12 p40 transcripts, nor stimulated IL-12 p40 promoter activity after IFN-gamma/LPS stimulation, although induction of other inducible genes was normal in these cells. Transfection of ICSBP led to a marked induction of both human and mouse IL-12 p40 promoter activities in ICSBP+/+ and ICSBP-/- cells, even in the absence of IFN-gamma/LPS stimulation. Whereas IRF-1 alone was without effect, synergistic enhancement of promoter activity was observed following cotransfection of ICSBP and IRF-1. Deletion analysis of the human promoter indicated that the Ets site, known to be important for activation by IFN-gamma/LPS, also plays a role in the ICSBP activation of IL-12 p40. A DNA affinity binding assay revealed that endogenous ICSBP is recruited to the Ets site through protein-protein interaction. Last, transfection of ISCBP alone led to induction of the endogenous IL-12 p40 mRNA in the absence of IFN-gamma and LPS. Taken together, our results show that ICSBP induced by IFN-gamma/LPS, acts as a principal activator of IL-12p40 transcription in macrophages.
The Total Synthesis of 6-Demethyl-6-Deoxytetracycline (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Total Synthesis of 6-Demethyl-6-DeoxytetracyclineL. H. Conover, K. Butler, J. D. Johnston, J. J. Korst, and R. B. WoodwardCite this: J. Am. Chem. Soc. 1962, 84, 16, 3222–3224Publication Date (Print):August 1, 1962Publication History Published online1 May 2002Published inissue 1 August 1962https://pubs.acs.org/doi/10.1021/ja00875a063https://doi.org/10.1021/ja00875a063research-articleACS PublicationsRequest reuse permissionsArticle Views1107Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Epidemiology of senior psychiatrists (1990)

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.