gebhart/patrepeval huggingface dataset:
A Novel Method for Isolating Specific Endocytic Vesicles Using Very Fine Ferrite Particles Coated with Biological Ligands and the High-Gradient Magnetic Separation Technique1 (1986)

We have developed a novel method for isolating specific endocytic vesicles using magnetic ligands and high-gradient magnetic separation. Ligands were prepared by coating extremely fine ferrite particles (10-20 nm) with bovine serum albumin and then conjugating asialoglycopeptides. These ligands were introduced into rat liver by perfusion at 16 or 37 degrees C, or by injection through the tail vein. The ligand particles were observed as electron-dense small grains in membrane-bound vesicles in Kupffer as well as parenchymal cells by electron microscopy. Livers were taken out, homogenized and lightly centrifuged. The supernatant was pumped into a separator glass tube filled with very fine ferritic stainless steel fibers and placed in a magnetic field of 0.9-2 T. Vesicles containing ferrite particles were collected with a high efficiency (ca. 70% of endocytosed magnetic ligands). About 70% of uptake appeared to be mediated by the asialoglycoprotein receptors. The captured vesicles were practically free from marker enzymes for plasma membranes, endoplasmic reticulum, and Golgi apparatus. Lysosomal enzyme activity of the vesicles increased with the time of perfusion at 37 degrees C but not at 16 degrees C. Protein composition of the captured vesicles was analyzed by one- and two-dimensional gel electrophoresis. The composition changed characteristically with time on perfusion at 16 and 37 degrees C. The present method provides a powerful tool to collect prelysosomal endocytic vesicles containing specific ligands and lysosomes fused with these specific endocytic vesicles.
Effects of General Anesthesia on High-Frequency Oscillations in Somatosensory Evoked Potentials (2006)

To determine the characteristics of high-frequency oscillations (HFOs) of cortical somatosensory evoked potentials (SEPs), the effect of general anesthesia on HFOs and low-frequency primary cortical responses was studied. The authors recorded SEPs elicited by median nerve stimulation directly from human brains of seven patients who underwent implantation of subdural electrodes before surgical treatment of intractable epilepsy. Recordings were made before and during general anesthesia. Changes in the number of HFOs and amplitude ratios of HFOs/primary cortical responses were analyzed. Under general anesthesia, the number of HFO peaks and the amplitude ratios were significantly decreased. General anesthesia induced remarkably decreased HFO activities when compared to low-frequency SEPs, suggesting that each of those originated from different generators. Possible relations between gamma-amino-butyric acid (GABA)ergic inhibitory interneurons and HFOs are discussed.
Cancer in the nervous system (1996)

Genetic and hereditary syndromes associated with the central nervous system diagnostic imaging: magnetic resonance imaging and computed tomography, nuclear medicine studies primary central nervous system tumours of the supratentorial compartment primary spinal cord tumours optic nerve, chiasmal and hypothalmic tumours tumours of the fourth ventrical pineal region tumours intracranial extra-axial tumours: meningiomas, intracranial nerve sheath tumours, skull base chordomas pituitary tumours intracranial metastic central nervous system tumours spinal axis metastases leptomeningeal metastases: presentation, diagnosis and management considerations, pharmacologic approaches to treatment altered consciousness in the cancer patient altered mental status due to structural disease, seizures and syncope, brain death the effects of cancer on the peripheral and central nervous system and its treatment: peripheral neuropathy in cancer patient, plexopathies in cancer patients, paraneoplastic neurological disorders cerebrovascular disease in the cancer patient psychiatric and psychosocial issues in neuro-oncology the assessment and management of pain in the cancer patient symptom management rehabilitation strategies, and improved quality of life for patients with brain tumours.
A genetic screen for mutations affecting embryogenesis in zebrafish. (1996)

Systematic genome-wide mutagenesis screens for embryonic phenotypes have been instrumental in the understanding of invertebrate and plant development. Here, we report the results from the first application of such a large-scale genetic screening to vertebrate development. Male zebrafish were mutagenized with N-ethyl N-nitrosourea to induce mutations in spermatogonial cells at an average specific locus rate of one in 651 mutagenized genomes. Mutations were transmitted to the F1 generation, and 2205 F2 families were raised. F3 embryos from sibling crosses within the F2 families were screened for developmental abnormalities. A total of 2337 mutagenized genomes were analyzed, and 2383 mutations resulting in abnormal embryonic and early larval phenotypes were identified. The phenotypes of 695 mutants indicated involvement of the identified loci in specific aspects of embryogenesis. These mutations were maintained for further characterization and were classified into categories according to their phenotypes. The analyses and genetic complementation of mutations from several categories are reported in separate manuscripts. Mutations affecting pigmentation, motility, muscle and body shape have not been extensively analyzed and are listed here. A total of 331 mutations were tested for allelism within their respective categories. This defined 220 genetic loci with on average 1.5 alleles per locus. For about two-thirds of all loci only one allele was isolated. Therefore it is not possible to give a reliable estimate on the degree of saturation reached in our screen; however, the number of genes that can mutate to visible embryonic and early larval phenotypes in zebrafish is expected to be several-fold larger than the one for which we have observed mutant alleles during the screen. This screen demonstrates that mutations affecting a variety of developmental processes can be efficiently recovered from zebrafish.
Mutation of Succinate Dehydrogenase Subunit C Results in Increased O2·−, Oxidative Stress, and Genomic Instability (2006)

Mutations in genes coding for succinate dehydrogenase (SDH) subunits are believed to contribute to cancer and aging, but the mechanism for this is unclear. Hamster fibroblasts expressing a mutation in SDH subunit C (SDHC; B9) showed 3-fold increases in dihydroethidine and dichlorodihydrofluorescein (CDCFH(2)) oxidation indicative of increased steady-state levels of O2(.-) and H2O2, increases in glutathione/glutathione disulfide (indicative of oxidative stress), as well as increases in superoxide dismutase activity, relative to parental B1 cells. B9 cells also showed characteristics associated with cancer cells, including aneuploidy, increases in glucose consumption, and sensitivity to glucose deprivation-induced cytotoxicity. Expression of wild-type (WT) human SDHC in B9 cells caused prooxidant production, glucose consumption, sensitivity to glucose deprivation-induced cytotoxicity, and aneuploidy to revert to the WT phenotype. These data show that SDHC mutations cause increased O2(.-) production, metabolic oxidative stress, and genomic instability and that mutations in genes coding for mitochondrial electron transport chain proteins can contribute to phenotypic changes associated with cancer cells. These results also allow for the speculation that DNA damage to genes coding for electron transport chain proteins could result in a "mutator phenotype" by increasing steady-state levels of O2(.-) and H2O2.
The Growth Hormone Dependent Serine Protease Inhibitor, Spi 2.1 Inhibits the Des (1-3) Insulin-Like Growth Factor-I Generating Protease (1997)

The conversion of insulin-like growth factor-I (IGF-I) to the biologically more active des (1-3) IGF-I variant is catalyzed by a ubiquitous protease. This proteolytic activity is inhibited by humanα 1-antitrypsin and soy-bean trypsin inhibitor and is up-regulated in serum and tissue extracts of hypophysectomized rats. These observations lead us to investigate whether the growth hormone regulated, serine protease inhibitor, Spi 2.1 was able to inhibit the des (1-3) IGF-I generating protease. Dihydrofolate reductase deficient Chinese hamster ovary (CHOdhfr-ve) cells were transfected with a rat Spi 2.1 expression vector containing the dhfr and neomycin resistance gene. Stable transfectants were selected using G418 and amplified using methotrexate. Conditioned medium from Spi 2.1 transfected CHO cells potently inhibited proteolytic activity directed against a synthetic hexa-peptide with a sequence identical to the N-terminal of IGF-I. In contrast conditioned medium from wild-type CHO cells had little effect. Based upon these observations we suggest that our previous finding of enhanced des (1-3) IGF-I generating protease activity in growth hormone deficient rats may be, at least partly explained by reduced levels of Spi 2.1. Furthermore, we propose that the regulation of the generation of des (1-3) IGF-I may be an additional potential site of growth hormone regulation of IGF-I action.
A BETTER SENSOR FOR NERVE GAS (2003)

RETURN TO ISSUEPREVNewsNEXTA BETTER SENSOR FOR NERVE GASFluorescence system detects analytes by their reactivity with indicator dyeRON DAGANICite this: Chem. Eng. News 2003, 81, 10, 12Publication Date (Print):March 10, 2003Publication History Published online13 November 2010Published inissue 10 March 2003https://pubs.acs.org/doi/10.1021/cen-v081n010.p012https://doi.org/10.1021/cen-v081n010.p012newsACS PublicationsCopyright © 2003 AMERICAN CHEMICAL SOCIETYArticle Views16Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Molecules Get e-Alerts
Neue Synthesemethoden, 10. β‐Ketosulfone als Ethylencarbanionen‐Äquivalente: die Synthese ungesättigter Carbonsäuren (1984)

Abstract α‐Alkylthiolierte Cycloalkanone werden nach Oxidation zu Sulfonen unter Halogenierung zu α‐Halogensulfonen ringgeöffnet. Ramberg‐Bäcklund‐Umlagerung ergibt ungesättigte Carbonsäuren, wobei die Lage der neuen Doppelbindung durch die Ringgröße der Ausgangscycloalkanone bestimmt wird.
Ultrafast phase dynamics of coherent emission from excitons in GaAs quantum wells (1994)

We investigate the temporal evolution of the instantaneous frequency of ultrashort-pulse four-wave mixing in GaAs/${\mathrm{Ga}}_{\mathit{x}}$${\mathrm{Al}}_{1\mathrm{\ensuremath{-}}\mathit{x}}$As quantum-well structures. We find that the coherent light emitted by excitons exhibits a complex phase behavior which depends critically on the density of excitons and the central frequency of the exciting pulses. Depending on the excitation conditions, we observe nonlinear shifts of the instantaneous frequency within one ultrashort pulsed emission or quantum beats. In the latter case we determine that the beat \ensuremath{\pi}-phase shift is very fast, only 40% above the fundamental quantum limit. We also present elaborate numerical simulations of the experiments based on a six-band generalization of the semiconductor Bloch equations. This formalism takes into account Coulomb many-body interactions and Pauli exclusion. It reproduces the salient features of the experimental data. It fails to account for important details revealed by the very sensitive phase measurements. The discrepancies can be traced back to two approximations in the theory: the statical treatment of screening, and the Lorentzian description of dephasing. In both cases, this indicates the need for theoretical refinements requiring a microscopic description of Coulomb scattering and dephasing processes in a non-Markovian theory.
Adenovirus and adeno-associated virus mediated gene transfer (1995)

In this review we describe current strategies for adenoviral mediated gene transfer (AMGT) and adeno-associated viral mediated gene transfer (AAVMGT). We consider the structure and molecular biology of adenoviruses and adeno-associated viruses and detail the current advantages and disadvantages of AMGT and AAVMGT. Potential solutions to some of the specific drawbacks to AMGT, including the development of new vectors, addition of gp19k, organoides, and the use of non-human adenoviral vectors, are discussed.
Tumor necrosis factor and interleukin-6 in Candida albicans infection in normal and granulocytopenic mice (1992)

We administered a neutralizing monoclonal antibody to tumor necrosis factor (TNF) during infection with Candida albicans in normal and granulocytopenic mice. Mice were rendered granulocytopenic (less than 0.1 x 10(9) granulocytes per liter) with cyclophosphamide. Growth of C. albicans from the kidneys was significantly increased in normal mice treated with the antibody to TNF, compared with that in control mice, after 36 h (3.6 x 10(4) +/- 1.2 x 10(4) CFU per kidney versus 9.1 x 10(3) +/- 6.2 x 10(3) CFU per kidney; P less than 0.05) and after 72 h (3.7 x 10(6) +/- 2.7 x 10(6) CFU per kidney versus 2.3 x 10(4) +/- 1.3 x 10(4) CFU per kidney; P less than 0.01). In granulocytopenic mice, the antibody to TNF had no effect on the growth of C. albicans from the kidneys. Furthermore, our study showed that the cytokines TNF and interleukin-6 (IL-6) were produced in a dose-dependent manner during C. albicans infection. TNF was detectable between 6 and 60 h, with peak levels at 24 h. Both TNF and IL-6 levels were significantly higher in cyclophosphamide-treated mice than in normal mice. Heat-inactivated C. albicans induced a TNF response different from that induced by viable C. albicans, with an early peak occurring at 3 to 4 h and declining to non-detectable levels after 15 to 24 h. Peak levels of TNF obtained with heat-inactivated C. albicans were lower than those obtained with viable C. albicans. Our study demonstrates that TNF and IL-6 are produced systemically during C. albicans infection and suggests that TNF is essential for granulocyte antifungal activity in vivo.
Identification of small-molecule antagonists that inhibit an activator:coactivator interaction (2004)

Phosphorylation of the cAMP response element binding protein (CREB) at Ser-133 in response to hormonal stimuli triggers cellular gene expression via the recruitment of the histone acetylase coactivator paralogs CREB binding protein (CBP) and p300 to the promoter. The NMR structure of the CREB:CBP complex, using relevant interaction domains called KID and KIX, respectively, reveals a shallow hydrophobic groove on the surface of KIX that accommodates an amphipathic helix in phospho (Ser-133) KID. Using an NMR-based screening approach on a preselected small-molecule library, we identified several compounds that bind to different surfaces on KIX. One of these, KG-501 (2-naphthol-AS-E-phosphate), targeted a surface distal to the CREB binding groove that includes Arg-600, a residue that is required for the CREB:CBP interaction. When added to live cells, KG-501 disrupted the CREB: CBP complex and attenuated target gene induction in response to cAMP agonist. These results demonstrate the ability of small molecules to interfere with second-messenger signaling cascades by inhibiting specific protein-protein interactions in the nucleus.
Insulin Receptor Substrate-1 is the Predominant Signaling Molecule Activated by Insulin-like Growth Factor-I, Insulin, and Interleukin-4 in Estrogen Receptor-positive Human Breast Cancer Cells (1998)

Because insulin-like growth factor-I (IGF-I), insulin, and interleukin-4 (IL-4) have known biological effects in breast cancer cells and signal through insulin-receptor substrate (IRS) adaptor proteins, we examined the expression and function of IRS-1 and IRS-2 in breast tumors and cell lines. IRS-1 and IRS-2 were expressed by cell lines and primary breast tumor specimens. IGF-I, insulin, and IL-4 treatment of MCF-7 and ZR-75, and IGF-I treatment of T47-D breast cancer cells, resulted in much greater tyrosine phosphorylation of IRS-1 compared with IRS-2. Furthermore, IGF-I stimulated greater tyrosine phosphorylation of IRS-1 than either insulin or IL-4. IGF-I treatment also enhanced association of the p85 regulatory subunit of phosphatidylinositol 3-kinase with IRS-1 and stimulated increased enzymatic activity compared with IL-4 and insulin in all three cell lines. Similarly, mitogen-activated protein kinase activity was greater in IGF-I-stimulated cells. To determine the functional significance of the activation of these pathways, we inhibited activation of phosphatidylinositol 3-kinase with wortmannin and mitogen-activated protein kinase with PD098059. Both compounds inhibited IGF-stimulated growth, suggesting that both pathways contributed to the mitogenic response to IGF-I. We conclude that IRS-1, and not IRS-2, is the predominant signaling molecule activated by IGF-I, insulin, and IL-4. Furthermore, enhanced tyrosine phosphorylation of IRS-1 by IGF-I, compared with either insulin or IL-4, is associated with greater activation of mitogenic downstream signaling pathways resulting in enhanced cell growth.
Polyriboinosinic Polyribocytidylic Acid (Poly(I:C)) Induces Stable Maturation of Functionally Active Human Dendritic Cells (1999)

For vaccination strategies and adoptive immunotherapy purposes, immature dendritic cells (DC) can be generated from adherent monocytes using GM-CSF and IL-4. Presently, the only clinically applicable method to induce stable maturation of DC is the use of supernatants of activated monocytes (monocyte-conditioned medium (MCM)). MCM contains an undefined mixture of cytokines and is difficult to standardize. Here we report that stable maturation of DC can be simply induced by the addition of polyriboinosinic polyribocytidylic acid (poly(I:C)), a synthetic dsRNA clinically applied as an immunomodulator. Poly(I:C)-treated DC show a mature phenotype with high expression levels of HLA-DR, CD86, and the DC maturation marker CD83. This mature phenotype is retained for 48 h after cytokine withdrawal. In contrast to untreated DC, poly(I:C)-treated DC down-regulate pinocytosis, produce high levels of IL-12 and low levels of IL-10, induce strong T cell proliferation in a primary allo MLR, and effectively present peptide Ags to HLA class I-restricted CTL. In conclusion, we present a simple methodology for the preparation of clinically applicable mature DC.
Advanced signal processing for misfire detection in automotive engines (2002)

The paper presents an application of artificial neural networks to the reliable detection of misfires in automotive engines. By government regulations, automobiles an required to be equipped with instrumentation to detect engine misfires and to alert the driver whenever the misfire rate has the potential to affect the health of emission control systems. A relevant model for the powertrain dynamics is developed as well as an explanation of the instrumentation. The basis for using a neural network to detect these misfires is explained and experimental system performance data (including error rates) an given. It is shown that the present method has the potential to meet the government mandated requirements.
A monolithic IGBT gate driver implemented in a conventional 0.8 μm BiCMOS process (2002)

This paper discusses the design and implementation of a monolithic gate driver for an insulated gate bipolar transistor (IGBT). The objective is to implement a high voltage (25 V) monolithic gate driver with an efficient protection circuit in a conventional low-voltage (5 V) high-density (0.8 /spl mu/m) BiCMOS process. Extended drain MOSFETs are used to implement the high-voltage capability in this design.
LATERAL INJECTION LASERS (1998)

Since its invention in the 60's, the semiconductor laser has relied on vertical injection of electrons and holes in order to pump the active region to inversion. While many aspects of semiconductor laser development have seen tremendous innovation and marked progress, this vertical injection scheme has remained unchanged. In contrast, the electronic transistor has evolved from the early dominance of vertical injection to today's largely lateral injection CMOS platform, which yielded new performance, functionality, and integrability and enabled the astonishingly successful integrated circuit revolution. The lateral current injection laser offers advantages which go far beyond optoelectronic integrability: it enables new functionalities, post-fabrication processing and engineering, and in some aspects of normal operation it can lead to greatly improved performances. To realize its full potential, this new class of semiconductor lasers deserves the same attention and the intensive iterations of efforts in theory, design, fabrication, and experimental probing as its vertical cousin has been received. We introduce a first-principles, combined theoretical-experimental approach to the exploration of the LCI laser. It reveals the role of lateral ambipolar drift-diffusion of carriers in either hindering or, potentially, in enhancing the provision of gain to the desired optical mode; of two-dimensional bandstructure engineering of the injection path and active region to achieve low differential resistance and efficient modal gain provision; of selective formation and lateral positioning of heterojunction in enabling high-efficiency device operation; and of adiabatic injection of electrons and holes across diffusively-graded heterojunctions in facilitating carrier capture into 2D quantum well states. Our results point to the tremendous promise of lasers based on lateral injection of current in enabling vertical cavity lasers with vastly increased performance and functional diversity; multi-terminal devices such as lasers with capacitive gain and wave-length tunability; high-speed directly-modulated lasers with reduced chirp; and functional devices with directly integrated high-speed longitudinal modulation.
Generation of Elastic Waves by Transient Surface Heating (1963)

When the surface of a body is subjected to transient heating (e.g., by electron bombardment or rf absorption) elastic waves are produced as a result of surface motion due to thermal expansion. This process is analyzed, with particular emphasis on the case of an input heat flux varying harmonically with time, to relate the elastic wave amplitude to the characteristics of the input flux and the thermal and elastic properties of the body. Experiments performed with both electron impact and rf absorption verify the proportionality of the stress wave amplitude and the absorbed power density, and correlate well with the thermal and elastic properties of the heated medium. Comparison of the elastic wave stress amplitude with radiation pressure shows that the former may be much greater than the latter, as experiments have demonstrated. When a barium titanate crystal was used to detect the elastic waves produced, heating by a single 2-μsec pulse of electrons or microwave radiation produced easily detectible signals at power levels down to 2 W/cm2, corresponding to a computed peak surface temperature rise of about 0.001°C, and produced piezoelectric crystal voltages ranging from about one to more than 60 mV/kW/cm2 absorbed power density.