gebhart/patrepeval huggingface dataset:
Activation of guanylate cyclase by superoxide dismutase and hydroxyl radical: a physiological regulator of guanosine 3',5'-monophosphate formation. (1977)

Partially purified soluble rat liver guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] was activated by superoxide dismutase (superoxide: superoxide oxidoreductase, EC 1.15.1.1). This activation was prevented with KCN or glutathione, inhibitors of superoxide dismutase. Guanylate cyclase preparations formed superoxide ion. Activation by superoxide dismutase was further enhanced by the addition of nitrate reductase. Although guanylate cyclase activity was much greater with Mn2+ than with Mg2+ as sole cation cofactor, activation with superoxide dismutase was not observed when Mn2+ was included in incubations. Catalase also decreased the activation induced with superoxide dismutase. Thus, activation required the formation of both superoxide ion and H2O2 in incubations. Activation of guanylate cyclase could not be achieved by the addition of H2O2 alone. Scavengers of hydroxyl radicals prevented the activation. It is proposed that superoxide ion and hydrogen peroxide can lead to the formation of hydroxyl radicals that activate guanylate cyclase. This mechanism of activation can explain numerous observations of altered guanylate cyclase activity and cyclic GMP accumulation in tissues with oxidizing and reducing agents. This mechanism will also permit physiological regulation of guanylate cyclase and cyclic GMP formation when there is altered redox or free radical formation in tissues in response to hormones, other agents, and processes.
Early Volume Expansion During Diarrhea and Relative Nephroprotection During Subsequent Hemolytic Uremic Syndrome (2011)

Intravenous volume expansion is an underused intervention that could decrease the frequency of oligoanuric renal failure in patients at risk of HUS.
Response of broilers to graded levels of microbial phytase added to maize–soyabean-meal-based diets containing three levels of non-phytate phosphorus (1996)

Male 1-d-old broilers (n 920) were given 0, 200, 400, 600, 800, 1000 and 1200 U microbial phytase/kg diet in combination with 2.0, 2.7 or 3.4 g non-phytate P (nP)/kg or 4.0, 5.1 or 5.8 g total P (tP)/kg in a 21 d trial to assess the effectiveness of phytase in a maize-soyabean-meal diet. In addition to the above twenty-one diets, a positive control P diet supplied 4.5 g nP/kg, 6.9 g tP/kg and 10 g Ca/kg. The basal diet contained 230 g crude protein/kg, 8.8 g Ca/kg, 4.4 g tP/kg and 2.0 g nP/kg. Defluorinated phosphate and limestone were used to supply P and Ca. A Ca:tP ratio of 2:1 was maintained except in the positive control diet which had a ratio of 1.45:1. Phytase additions linearly increased (P < 0.01) body-weight (BW) gain, feed intake, toe ash percentage, and apparent retention (% of intake) or total amount (g/bird) of retained Ca and P, and linearly decreased (P < 0.01) P excretion (g/kg of DM intake) at each level of nP with the magnitude of the response inversely related to the level of nP. Above-normal mortality was only observed in the group receiving 2.0 g nP/kg diet without phytase. Adding nP linearly increased (P < 0.01) BW gain, feed intake, toe ash percentage, Ca retention, total amount (g/bird) of P retained, and P excretion, and linearly decreased (P < 0.01) apparent retention (%) of P. Derived linear and non-linear equations for BW gain and toe ash percentage at the two lower nP levels, 2.0 and 2.7 g/kg, were used to calculate P equivalency values of microbial phytase. The results show that 939 U microbial phytase is equivalent to 1 g P from defluorinated phosphate in broilers fed on maize-soyabean-meal diets. The amount of P released per 100 U phytase decreased as the total amount of phytase increased.
The substantia nigra and ventral tegmental area in Alzheimer's disease and Down's syndrome. (1989)

Degenerative changes in the substantia nigra and ventral tegmental area were investigated in 104 cases of Alzheimer's disease and 13 cases of Down's syndrome. Frequencies of tangles in three groups of patients with Alzheimer's disease were 86%, 44% and 46% (54% overall) respectively. About half of those with tangles, but no Lewy bodies, had excess nigral cell loss, and 16% had moderate or severe neuronal fallout, but none had a Parkinsonian syndrome. Cases with nigral tangles were younger, tended to have more hippocampal and cortical tangles and plaques, and lower activities of cortical choline acetyltransferase. In most cases of Alzheimer's disease degeneration in nigral and tegmental areas was greater than controls, and tangles were evenly distributed. All the cases of Down's syndrome had tangles in the nigra and eight showed mild cell loss. Mild degenerative changes accompanied by tangles in the substantia nigra and ventral tegmental area are common in Alzheimer's disease, but severe cell loss is rare. When a Parkinsonian syndrome occurs in Alzheimer's disease it is likely to be due to Lewy body pathology.
Commentary and Opinion: II. Statistical Parametric Mapping: Ontology and Current Issues (1995)

This article was written at the invitation of the Editor, in response to the preceding article by Strother et aI. (1995). I have taken this opportunity to comment on several issues, some of which have been the subject of recent exchanges (e.g. , Taylor et aI., 1993; Worsley et aI. , 1993). Others deserve mention because they have become of special cur­ rent interest. An overview of the development of statistical analysis in functional imaging is pre­ sented, followed by a discussion of some key areas.
Review of Metamodeling Techniques in Support of Engineering Design Optimization (2006)

Computation-intensive design problems are becoming increasingly common in manufacturing industries. The computation burden is often caused by expensive analysis and simulation processes in order to reach a comparable level of accuracy as physical testing data. To address such a challenge, approximation or metamodeling techniques are often used. Metamodeling techniques have been developed from many different disciplines including statistics, mathematics, computer science, and various engineering disciplines. These metamodels are initially developed as “surrogates” of the expensive simulation process in order to improve the overall computation efficiency. They are then found to be a valuable tool to support a wide scope of activities in modern engineering design, especially design optimization. This work reviews the state-of-the-art metamodel-based techniques from a practitioner’s perspective according to the role of metamodeling in supporting design optimization, including model approximation, design space exploration, problem formulation, and solving various types of optimization problems. Challenges and future development of metamodeling in support of engineering design is also analyzed and discussed.
Nitric oxide/nucleophile complexes inhibit the in vitro proliferation of A375 melanoma cells via nitric oxide release. (1993)

Cell-mediated antitumor effects have, in part, been attributed to the production of NO. Compounds which generate NO might, therefore, be useful in attenuating the growth of tumor cells. Six nitric oxide/nucleophile adducts that release NO spontaneously in solution were tested for their effectiveness in inhibiting DNA synthesis in A375 human melanoma cells. The complexes of NO with spermine, 3-(n-propylamino)propylamine (PAPA/NO), and diethylamine reduced thymidine incorporation by 50% at concentrations of 24, 44, and 128 microM, respectively. The degree of inhibition was, in general, related to the rate and extent of NO release in solution. A melanoma cell clone sensitive to interleukin 1-mediated cytostasis (A375-C6) was no more sensitive to PAPA/NO than a clone resistant to interleukin 1 (A375-C5), suggesting that the differing inhibitory effects of interleukin 1 in the two A375 cell clones are not due to a differential sensitivity to nitric oxide. Oxymyoglobin (125 microM), a known scavenger of NO, restored the ability of A375-C6 cells to incorporate thymidine in the presence of up to 200 microM PAPA/NO. When PAPA/NO was added to a solution of oxymyoglobin, nitrosylmyoglobin was formed, indicating that the protective effect of myoglobin was due to scavenging of NO. The results are consistent with a nitric oxide-mediated mechanism for NO/nucleophile cytostasis and suggest that such compounds may be useful as tools for investigating the role of reactive nitrogen intermediates in cytostasis and cytotoxicity.
Arterial Thromboembolic Events in Patients with Metastatic Carcinoma Treated with Chemotherapy and Bevacizumab (2007)

Combination treatment with bevacizumab and chemotherapy, compared with chemotherapy alone, was associated with an increased risk of arterial thromboembolism but not venous thromboembolism.
Robust tracking-by-detection using a detector confidence particle filter (2009)

We propose a novel approach for multi-person tracking-by-detection in a particle filtering framework. In addition to final high-confidence detections, our algorithm uses the continuous confidence of pedestrian detectors and online trained, instance-specific classifiers as a graded observation model. Thus, generic object category knowledge is complemented by instance-specific information. A main contribution of this paper is the exploration of how these unreliable information sources can be used for multi-person tracking. The resulting algorithm robustly tracks a large number of dynamically moving persons in complex scenes with occlusions, does not rely on background modeling, and operates entirely in 2D (requiring no camera or ground plane calibration). Our Markovian approach relies only on information from the past and is suitable for online applications. We evaluate the performance on a variety of datasets and show that it improves upon state-of-the-art methods.
Genital human papillomavirus infection in female university students as determined by a PCR-based method (1991)

The presence of genital human papillomavirus (HPV) was determined at cervical and vulvar sites using two methods, the Food and Drug Administration—approved Vira Pap test and polymerase chain reaction (PCR) DNA amplification technology, in 467 women presenting to a university health service for a routine annual gynecologic examination. The PCR system afforded the sensitive detection of a broad spectrum of genital HPV types. Using PCR, we found that 46% of the study population was infected with HPV; the ViraPap test showed a prevalence of 11% infected. PCR analyses demonstrated that 69% of the HPV-positive women were infected at both genital sites. Subsequent HPV-type determination showed that 33% of the study population had HPV types 6, 11, 16, 18,31,33,35,39,45,51,52, or other previously isolated types, and 13% had yet unidentified types. Almost all (92%) of the women diagnosed by Papanicolaou smear with condylomatous atypia or dysplasia (n = 12) were HPV positive. The PCR method proved to be an informative and rapid way to detect HPV in large numbers of clinical samples. Our results demonstrate that genital HPV infection is common among sexually active young women. (<i>JAMA</i>. 1991;265:472-477)
Detection of bacteria in the presence of antibiotics by using specific monoclonal antibodies to neutralize the antibiotics (1988)

Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow in broth containing 0.25 micrograms of penicillin per ml and 4 micrograms of gentamicin per ml, respectively, when the specific antibodies were added. This procedure may be useful to increase the yield of bacteria from body fluid specimens that contain antibiotics.
Convenient, Rapid Synthesis of Ag Nanowires (2007)

The rapid, microwave-assisted aerobic synthesis of silver nanowires based on the polyol method is described. Benchtop dissolution of NaCl and AgNO3 (ratio 1:6 to 1:3) in ethylene glycol and subsequent heating using microwave irradiation (300 W) in the presence of polyvinylpyrrolidone generates Ag nanowires in ∼80% yield in 3.5 min. Upon purification, microscopy (TEM, SEM) and powder X-ray diffraction reveal a uniform set of crystalline Ag nanowires with dimensions of 45 nm × 4−12 μm. Wire formation is highly dependent upon the microwave heating power, time, and NaCl:AgNO3 ratio because of the rapid heating process and the presence of O2 as an etching coreagent. Extended microwave heating causes the wires to fuse if in proximity or degrade to shorter wires presumably via the etching reaction. In the absence of O2/Cl-, the wires melt upon extended microwave heating (>4 min), suggesting that nanowire melting may contribute to the observed morphology under etching conditions. Compared to existing wet-chemical methods using traditional heating techniques, microwave irradiation not only rapidly heats, but dielectric heating of the growing wires also occurs, resulting in accelerated deposition of Ag0 at the wire ends. Furthermore, this high yielding preparation does not require any external seed crystals, precursors, or mechanical stirring and is conducted under ambient O2 conditions, leading to significant potential for the large-scale fabrication of Ag nanowires using this simple approach.
Characterization of Chemically Modified Hyperthermophilic Enzymes for Chemical Syntheses and Bioremediation Reactions (1999)

Remediation processes frequently involve species possessing limited solubility in water. We are interested in novel strategies that use molecularly modified enzymes with enhanced activity and stability for the remediation of recalcitrant compounds in organic solvents. The performance of naturally-occurring enzymes is usually quite limited in such organic environments. The primary objective of the current work is to gain a fundamental understanding of the molecular and catalytic properties of enzymes that have been chemically modified so that they are catalytically active and chemically-stable in organic solvents. The premise for this study is that stabilized and activated enzymes, which can function at hash chemical conditions, are optimally suited for bioremediation in nonaqueous media where substrates of interest are more soluble and processed with greater efficiency. This unique strategy is examined with respect to the degradation of chlorophenols and PCBs.
Tick-Borne Encephalitis with Hemorrhagic Syndrome, Novosibirsk Region, Russia, 1999 (2020)

Eight fatal cases of tick-borne encephalitis with unusual hemorrhagic syndrome were identified in 1999 in the Novosibirsk Region, Russia. To study these strains, we sequenced cDNA fragments of protein E gene from six archival formalin-fixed brain samples. Phylogenetic analysis showed tick-borne encephalitis variants clustered with a Far Eastern subtype (homology 94.7%) but not with the Siberian subtype (82%).
Alternative Activation of STAT1 and STAT3 in Response to Interferon-γ (2004)

Interferon-gamma (IFNgamma) is a pluripotent cytokine whose major biological effects are mediated through a pathway in which STAT1 is the predominant and essential transcription factor. STAT3 can also be activated weakly by IFNgamma, but the mechanism of activation and function of STAT3 as a part of the interferon response are not known. Here we show that STAT3 activation is much stronger and more prolonged in STAT1-null mouse embryo fibroblasts than in wild-type cells. In response to IFNgamma, SRC-family kinases are required to activate STAT3 (but not STAT1) through tyrosine phosphorylation, whereas the receptor-bound kinases JAK1 and JAK2 are required to activate both STATs. Tyrosine 419 of the IFNgamma receptor subunit 1 (IFNGR1) is required to activate both STATs, suggesting that STAT1 and STAT3 compete with each other for the same receptor phosphotyrosine motif. Activated STAT3 can replace STAT1 in STAT1-null cells to drive the transcription of certain genes, for example, socs-3 and c/ebpdelta, which have gamma-activated sequence motifs in their promoters. Work from Ian Kerr's laboratory reveals that the gp130-linked interleukin-6 receptor, which usually activates STAT3 predominantly, activates STAT1 efficiently when STAT3 is absent. Because STAT1 and STAT3 have opposing biological effects (STAT3 is an oncogene, and STAT1 is a tumor suppressor), the reciprocal activation of these two transcription factors in response to IFNgamma or interleukin-6 suggests that their relative abundance, which may vary substantially in different normal cell types, under different conditions or in tumors is likely to have a major impact on how cells behave in response to different cytokines.
The Graph Neural Network Model (2008)

Many underlying relationships among data in several areas of science and engineering, e.g., computer vision, molecular chemistry, molecular biology, pattern recognition, and data mining, can be represented in terms of graphs. In this paper, we propose a new neural network model, called graph neural network (GNN) model, that extends existing neural network methods for processing the data represented in graph domains. This GNN model, which can directly process most of the practically useful types of graphs, e.g., acyclic, cyclic, directed, and undirected, implements a function tau(G,n) is an element of IR(m) that maps a graph G and one of its nodes n into an m-dimensional Euclidean space. A supervised learning algorithm is derived to estimate the parameters of the proposed GNN model. The computational cost of the proposed algorithm is also considered. Some experimental results are shown to validate the proposed learning algorithm, and to demonstrate its generalization capabilities.
Surface Plasmon Resonance Permits in Situ Measurement of Protein Adsorption on Self-Assembled Monolayers of Alkanethiolates on Gold (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSurface Plasmon Resonance Permits in Situ Measurement of Protein Adsorption on Self-Assembled Monolayers of Alkanethiolates on GoldMilan Mrksich, George B. Sigal, and George M. WhitesidesCite this: Langmuir 1995, 11, 11, 4383–4385Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/la00011a034https://doi.org/10.1021/la00011a034research-articleACS PublicationsRequest reuse permissionsArticle Views1799Altmetric-Citations312LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cloning and functional expression of the first plant fatty acid elongase specific for Δ6-polyunsaturated fatty acids (2000)

In order to elucidate the biosynthesis of long-chain polyunsaturated fatty acids (PUFAs) in plants we searched for a cDNA encoding a Delta(6)-specific PUFA elongase from Physcomitrella patens, which is known to contain high proportions of arachidonic acid (20:4 Delta(5,8,11,14)). An EST clone from P. patens was identified by its low homology to the yeast gene ELO1, which is required for the elongation of medium-chain fatty acids. We functionally characterized this cDNA by heterologous expression in Saccharomyces cerevisiae grown in the presence of several fatty acids. Analysis of the fatty acid profile of the transgenic yeast revealed that the cDNA encodes a protein that leads to the elongation of the C(18) Delta(6)-polyunsaturated fatty acids gamma-linolenic acid (18:3 Delta(6,9,12)) and stearidonic acid (18:4 Delta(6,9,12,15)), which were recovered to 45-51% as their elongation products. In contrast, linoleic and alpha-linolenic acids were hardly elongated and we could not measure any elongation of saturated and mono-unsaturated fatty acids (including 18:1 Delta(6)), indicating that the elongase is highly specific for the polyunsaturated nature of the fatty acid acting as substrate.
Mobile Visual Search (2011)

Mobile phones have evolved into powerful image and video processing devices equipped with high-resolution cameras, color displays, and hardware-accelerated graphics. They are also increasingly equipped with a global positioning system and connected to broadband wireless networks. All this enables a new class of applications that use the camera phone to initiate search queries about objects in visual proximity to the user (Figure 1). Such applications can be used, e.g., for identifying products, comparison shopping, finding information about movies, compact disks (CDs), real estate, print media, or artworks.
Induction of protective polyclonal antibodies by immunization with a Plasmodium yoelii circumsporozoite protein multiple antigen peptide vaccine. (1995)

Monoclonal Abs against the repeat region of the circumsporozoite protein (CSP) completely protect mice against Plasmodium yoelii (Py), but synthetic peptide and recombinant protein vaccines designed to produce only Abs to the PyCSP repeat region have never been reported to consistently provide protection. This lack of protection in the rodent model system has predicted the poor protection achieved in humans after immunization with synthetic peptide and recombinant protein P. falciparum CSP vaccines and has raised serious questions regarding the capacity for vaccine-induced polyclonal Abs against the CSP to consistently protect humans. We now report immunization studies with a multiple Ag peptide vaccine designed to rely on "universal" T epitopes from tetanus toxin to produce T cell help for induction of protective Abs against the repeat region of the PyCSP. When delivered with a nonionic block co-polymer adjuvant, the vaccine protected 78 to 100% of three inbred strains of mice, and 100% of outbred mice against P. yoelii sporozoite challenge. Protection was associated with Ab titer, and passive transfer of purified IgG from immune mice protected naive recipients. Similar protection was achieved when the peptide was encapsulated in liposomes with lipid A and mixed with aluminum hydroxide. By demonstrating for the first time solid protection against P. yoelii by polyclonal Abs against the CSP, these data provide the rationale for assessment of a similarly constructed and formulated P. falciparum CSP multiple Ag peptide vaccine in humans.