gebhart/patrepeval huggingface dataset:
Taxation with Representation: Intergovernmental Grants in a Plebiscite Democracy (2006)

Economic theory predicts that unconditional intergovernmental grant income and private income are perfectly fungible. Despite this prediction, the literature on fiscal federalism documents that grant and private income are empirically non-equivalent. A large scale school finance reform in New Hampshire -- the typical school district experienced a 200 percent increase in grant income -- provides an unusually compelling test of the equivalence prediction. Most theoretical explanations for non-equivalence focus on mechanisms which produce public good provision levels which differ from the decisive voter&amp;amp;amp;#x27;s preferences. New Hampshire determines local public goods provision via a form of direct democracy -- a setting which rules out these explanations. In contrast to the general support in the literature for non-equivalence, the empirical estimates in this paper suggest that approximately 92 cents per grant dollar are spent on tax reduction. These results not only document that equivalence holds in a setting with a strong presumption that public good provision decisions reflect the preferences of voters, but also directly confirm the prediction of the seminal work of Bradford and Oates (1971) that lump-sum grant income is equivalent to a tax reduction. In addition, the paper presents theoretical arguments that grant income capitalization and heterogeneity in the marginal propensity to spend on public goods may generate spurious rejections of the equivalence prediction. The heterogeneity argument is confirmed empirically. Specifically, the results indicate that lower income communities spend more of the grant income on education than wealthier communities, a finding interpreted as revealing that the Engel curve for education is concave.
Intercellular trafficking of a KNOTTED1 green fluorescent protein fusion in the leaf and shoot meristem of Arabidopsis (2002)

Dominant mutations in the maize homeobox gene knotted1 (kn1) act nonautonomously during maize leaf development, indicating that Kn1 is involved in the generation or transmission of a developmental signal that passes from the inner layers of the leaf to epidermal cells. We previously found that this nonautonomous activity is correlated with the presence of KN1 protein in leaf epidermal cells, where KN1 mRNA could not be detected. Furthermore, KN1 protein expressed in Escherichia coli and labeled with a fluorescent dye can traffic between leaf mesophyll cells in microinjection assays. Here we show that green fluorescent protein (GFP)-tagged KN1 is able to traffic between epidermal cells of Arabidopsis and onion. When expressed in vivo, the GFP approximately KN1 fusion trafficked from internal tissues of the leaf to the epidermis, providing the first direct evidence, to our knowledge, that KN1 can traffic across different tissue layers in the leaf. Control GFP fusions did not show this intercellular trafficking ability. GFP approximately KN1 also trafficked in the shoot apical meristem, suggesting that cell-to-cell trafficking of KN1 may be involved in its normal function in meristem initiation and maintenance.
A vertically integrated eutrophication model and its application to a river-style reservoir - Fuchunjiang, China (2009)

基于一个 2-D 水动力学模型，一个垂直地综合的超营养作用模型被开发。物理亚模型能在不同深度被用于水密度的计算，并且水质量亚模型被用于海藻的生长的计算。连贯、非连贯的沉积与不同方法独立被模仿。轻扑灭系数使用了在在水下轻政体亚模型是线性地与沉积和浮游植物生物资源的和有关。对模型重要的一些部件更少是 simpliffied 改进有实行可能和计算效率。用从 Fuchunjiang 水库的 ffield 数据，我们计算了在这个模型包括并且验证了模型的生态的参数的敏感。敏感分析的结果证明参数强烈影响了浮游植物生物资源，包括浮游植物最大值生长率，呼吸率，非食肉的死亡率，安定的率，浮游动物最大值 ffiltration 率，为推迟的固体的 speciffic 扑灭系数和沉积氧需求率。模型被调整这些参数校准。全部的叶绿素一(chl -- 一) 在在水列的不同的层的集中被模型模拟复制很好。模仿的 chl -- 一价值断然与 0.92 的皮尔森关联系数被相关到测量价值。测量并且模仿的 chl 之间的吝啬的差别 -- 一集中是 12% 测量 chl -- 集中。测量并且模仿集中断然也被相关(r = 0.74 ) 并且吝啬的差别是中的 4% 个测量集中。模型的成功的确认显示它将为另外的河风格水库的水质量规定在 Fuchunjiang 水库和一个好工具在水质量管理和海藻的花蕾预言是很有用的。
Autophagy and cancer therapy (2011)

Autophagy is an evolutionarily conserved lysosomal pathway for the degradation of cytoplasmic proteins,macromolecules,and organelles.Now,autophagic cell death is considered as programmed cell death type Ⅱ.In multiple studies,inhibition of autophagy will result in contrasting outcomes-survival or death.Thus,whether autophagy in cancer cells causes death or protects cells is controversial.Taken together,the manipulation of autophagy may lead to development of new cancer therapies.This article focuses on recent progresses of autophagy research related to human cancers therapy.

Key words: 
Autophagy; Neoplasms; Therapy
Bandwidth-efficient continuous media streaming through optimal multiplexing (1999)

bandwidth efficiency in dist,ributed continuous media streaming systems is the key in delivering cost-effective mult,imedia services to distributed and heterogeneous receivers.
Binding, uptake, and intracellular trafficking of phosphorothioate-modified oligodeoxynucleotides. (1995)

An enhanced appreciation of uptake mechanisms and intracellular trafficking of phosphorothioate modified oligodeoxynucleotides (P-ODN) might facilitate the use of these compounds for experimental and therapeutic purposes. We addressed these issues by identifying cell surface proteins with which P-ODN specifically interact, studying P-ODN internalization mechanisms, and by tracking internalized P-ODN through the cell using immunochemical and ultrastructural techniques. Chemical cross-linking studies with a biotin-labeled P-ODN (bP-ODN), revealed the existence of five major cell surface P-ODN binding protein groups ranging in size from approximately 20-143 kD. Binding to these proteins was competitively inhibited with unlabeled P-ODN, but not free biotin, suggesting specificity of the interactions. Additional experiments suggested that binding proteins likely exist as single chain structures, and that carbohydrate moieties may play a role in P-ODN binding. Uptake studies with 35S-labeled P-ODN revealed that endocytosis, mediated by a receptor-like mechanism, predominated at P-ODN concentrations < 1 microM, whereas fluid-phase endocytosis prevailed at higher concentrations. Cell fractionation and ultrastructural analysis demonstrated the presence of ODN in clathrin coated pits, and in vesicular structures consistent with endosomes and lysosomes. Labeled ODN were also found in significant amounts in the nucleus, while none was associated with ribosomes, or ribosomes associated with rough endoplasmic reticulum (ER). Since nuclear uptake was not blocked by wheat germ agglutinin or concanavalin A, a nucleoporin independent, perhaps diffusion driven, import process is suggested. These data imply that antisense DNA may exert their effect in the nucleus. They also suggest rational ways to design ODN which might increase their efficiency.
A core–shell Pd1Ru1Ni2@Pt/C catalyst with a ternary alloy core and Pt monolayer: enhanced activity and stability towards the oxygen reduction reaction by the addition of Ni (2015)

A core–shell structured catalyst exhibits high activity and stability.
Role of the B domain for factor VIII and factor V expression and function (1994)

Factor V and factor VIII are homologous cofactors in the blood coagulation cascade that have the domain structure A1-A2-B-A3-C1-C2, of which the B domain has extensively diverged. In transfected COS-1 monkey cells, expression of factor VIII is approximately 10-fold less efficient than that of factor V, primarily because of inefficient protein secretion and, to a lesser extent, reduced mRNA expression. To study the functional significance and effect of the B domain on expression and activity, chimeric cDNAs were constructed in which the B domains of factor V and factor VIII were exchanged. Expression of a factor VIII chimera harboring the B-domain of factor V yielded a fully functional factor VIII molecule that was expressed twofold more efficiently than wild-type factor VIII because of increased mRNA expression. Thus, sequences within the factor VIII B domain were not responsible for the inefficient secretion of factor VIII compared with factor V. Expression of a factor V chimera harboring the B domain of factor VIII was slightly reduced compared with wild-type factor V, although the secreted molecule had significantly reduced procoagulant activity correlating with dissociated heavy and light chains and resistance to thrombin activation. Interestingly, the factor V chimera containing the factor VIII B domain was efficiently activated by Russell's viper venum (RVV). A factor V B domain deletion (residues 710-1545) molecule also exhibited significantly reduced procoagulant activity caused by resistance to thrombin cleavage and activation, although this molecule was activatable by RVV. These results show that, in contrast to factor VIII, thrombin activation of factor V requires sequences within the B domain. In addition, thrombin activation of factor V occurs through a different mechanism than activation by RVV.
Acylation-stimulating Protein (ASP) Deficiency Induces Obesity Resistance and Increased Energy Expenditure in ob/obMice (2002)

Acylation-stimulating protein (ASP) acts as a paracrine signal to increase triglyceride synthesis in adipocytes. ASP administration results in more rapid postprandial lipid clearance. In mice, C3 (the precursor to ASP) knockout results in ASP deficiency and leads to reduced body fat and leptin levels. The protective potential of ASP deficiency against obesity and involvement of the leptin pathway were examined in ob/ob C3(-/-) double knockout mice (2KO). Compared with age-matched ob/ob mice, 2KO mice had delayed postprandial triglyceride and fatty acid clearance; associated with decreased body weight (4-17 weeks age: male: -13.7%, female: -20.6%, p < 0.0001) and HOMA (homeostasis model assessment) index (-37.7%), suggesting increased insulin sensitivity. By contrast, food intake in 2KO mice was +9.1% higher over ob/ob mice (p < 0.001, 2KO 5.1 +/- 0.2 g/day, ob/ob 4.5 +/- 0.2 g/day, wild type 2.6 +/- 0.1 g/day). The hyperphagia/leanness was balanced by a 28.5% increase in energy expenditure (oxygen consumption: 2KO, 131 +/- 8.9 ml/h; ob/ob, 102 +/- 4.5 ml/h; p < 0.01; wild type, 144 +/- 8.9 ml/h). These results suggest that the ASP regulation of energy storage may influence energy expenditure and dynamic metabolic balance.
Localization and Action of Dragon (Repulsive Guidance Molecule b), a Novel Bone Morphogenetic Protein Coreceptor, throughout the Reproductive Axis (2005)

Bone morphogenetic proteins (BMPs) play important roles in reproduction including primordial germ cell formation, follicular development, spermatogenesis, and FSH secretion. Dragon, a recently identified glycosylphosphatidylinositol-anchored member of the repulsive guidance molecule family, is also a BMP coreceptor. In the present study, we determined the tissue and cellular localization of Dragon in reproductive organs using immunohistochemistry and in situ hybridization. Among reproductive organs, Dragon was expressed in testis, epididymis, ovary, uterus, and pituitary. In the testis of early postnatal mice, Dragon was found in gonocytes and spermatogonia, whereas in immature testes, Dragon was only weakly expressed in spermatogonia. Interestingly, pregnant mare serum gonadotropin treatment of immature mice robustly induced Dragon production in spermatocytes. In adult testis, Dragon was found in spermatocytes and round spermatids. In the ovary, Dragon was detected exclusively within oocytes and primarily those within secondary follicles. In the pituitary, Dragon-expressing cells overlapped FSH-expressing cells. Dragon was also expressed in a number of cell lines originating from reproductive tissues including Ishikawa, Hela, LbetaT2, MCF-7, and JEG3 cells. Immunocytochemistry and gradient sucrose ultracentrifugation studies showed Dragon was localized in lipid rafts within the plasma membrane. In reproductive cell lines, Dragon expression enhanced signaling of exogenous BMP2 or BMP4. The present studies demonstrate that Dragon expression is dynamically regulated throughout the reproductive tract and that Dragon protein modulates BMP signaling in cells from reproductive tissues. The overlap between Dragon expression and the functional BMP signaling system suggests that Dragon may play a role in mammalian reproduction.
Deposition of mass selected silver clusters in rare gas matrices (1990)

We report on the successful ‘‘soft landing’’ of size selected silver dimers and trimers in solid krypton matrices. Silver cluster cations, produced by sputtering, were mass selected in a quadrupole mass filter and then codeposited with krypton on a cooled sapphire or CaF2 window in the presence of low energy electrons. Neutralized cluster samples were interrogated in situ by excitation and fluorescence spectroscopy. Deposition of slow (≤20 eV) silver dimer cations gave rise to strong excitation bands (centered at λ=275 and 390 nm) from Ag2 plus the characteristic triplet signal of the atom. The spectra imply that fewer than 25% of the dimers were fragmented during the neutralization and deposition steps. In similar experiments with Ag3 we were able to assign trimer absorption bands at 331, 364, 402, 421, 458, and 514 nm and identify characteristic emission features at 381, 560, and 626 nm.
Contigra (2002)

Even though numerous Web3D technologies exist, most of them do not support a high-level, multi-disciplinary authoring process. Moreover, concepts of reuse are rarely provided. A component-based approach is introduced with the CONTIGRA architecture to construct interactive, three-dimensional applications, either stand-alone or web-based. The approach is entirely based on declarative XML documents describing the component implementation, its interface, as well as component configuration and composition of 3D user interfaces and virtual environments. Extensible 3D (X3D) is used as the scene graph basis. However, the resulting applications can be translated to other 3D technologies, too. Another advantage of the approach is reuse both at the implementation level and the higher abstract component level. This paper introduces the overall architecture and the XML schemas used for the component documents. It finally outlines the associated authoring process and tools involved.
Solid Lipid Particles for Lung Metastasis Treatment (2021)

Solid lipid particles (SLPs) can sustainably encapsulate and release therapeutic agents over long periods, modifying their biodistribution, toxicity, and side effects. To date, no studies have been reported using SLPs loaded with doxorubicin chemotherapy for the treatment of metastatic cancer. This study characterizes the effect of doxorubicin-loaded carnauba wax particles in the treatment of lung metastatic malignant melanoma in vivo. Compared with the free drug, intravenously administrated doxorubicin-loaded SLPs significantly reduce the number of pulmonary metastatic foci in mice. In vitro kinetic studies show two distinctive drug release profiles. A first chemotherapy burst-release wave occurs during the first 5 h, which accounts for approximately 30% of the entrapped drug rapidly providing therapeutic concentrations. The second wave occurs after the arrival of the particles to the final destination in the lung. This release is sustained for long periods (>40 days), providing constant levels of chemotherapy in situ that trigger the inhibition of metastatic growth. Our findings suggest that the use of chemotherapy with loaded SLPs could substantially improve the effectiveness of the drug locally, reducing side effects while improving overall survival.
Study of Singlet Oxygen Dynamics on Silicon Polymer Matrix (2019)

We report a detailed analysis of singlet oxygen generated from the photofunctional polymer film (PFPF) matrix which is the silicone polymer film (PDMS) embedded with a photosensitizer. Activation and deactivation dynamics of singlet oxygen generated from PFPFs were investigated with time-resolved phosphorescence spectroscopy. The singlet oxygen generated from PFPFs was dissipated into three different regions of the polymer matrix; the inside (component A), the surface (component B), and the outside (component C). According to the deactivation dynamics of singlet oxygen in the polymer matrix, the components B and C are expected to be more important for various applications.
The v-rel oncogene encodes a cell-specific transcriptional activator of certain promoters. (1988)

Transformation by the v-rel oncogene of avian reticuloendotheliosis virus strain T (Rev-T) is primarily cell-specific. While v-rel efficiently transforms chicken spleen and bone marrow stem cells in vitro and induces rapid lethal lymphomas in young birds, it does not rapidly transform chicken embryo fibroblasts. The nuclear localization of the v-rel gene product in non-transformed fibroblasts along with its ability to function as a transforming protein in the nucleus of chicken spleen cells suggests that p59v-rel might belong to the family of nuclear oncoproteins and thus may express an immortalizing function in fibroblasts. To gain insight into the specificity of cell transformation by the v-rel oncogene, we determined whether v-rel could immortalize primary rat fibroblasts. Our experiments have shown that, unlike other nuclear oncoproteins, p59v-rel did not immortalize primary rat embryo fibroblasts. However p59v-rel was able to cooperate in a synergistic way with the polyomavirus middle T protein in inducing efficient transformation of established rat fibroblasts by increasing the steady-state level of middle T RNA, indicating that p59v-rel might function as a transactivator. Cotransfection of cells from different species with the v-rel gene along with constructs expressing the chloramphenicol acetyl transferase gene under the control of different promoters revealed that p59v-rel is a cell-specific transcriptional transactivator of certain promoters. Moreover, the extent of cell-specific transactivation by v-rel correlated with its toxic effect in these same cells.
4.2: A Low Power Consumption and High Reliability Architecture for aSi TFT Gate Driver on Glass (2010)

Abstract We developed aSi TFT gate driver circuits on glass for low power solution and longterm reliability. The proposed circuit architecture achieved lower power consumption by means of switched capacitor bootstrap design. The degradation of aSi TFT was small and saturated until 200h of aging due to adopting this architecture. 26“WXGA panels were fabricated.
Emerging Role of RAB GTPases in Cancer and Human Disease (2005)

Emerging evidence implicates alterations in the RAB small GTPases and their associated regulatory proteins and effectors in multiple human diseases including cancer. We have recently shown that RAB25, located at chromosome 1q22, is amplified at the DNA level and overexpressed at the RNA level in ovarian and breast cancer. These changes correlated with a worsened outcome in both diseases. In addition, enforced expression of RAB25 in both breast and ovarian cancer cells decreased apoptosis and increased proliferation and aggressiveness in vivo, potentially explaining the worsened prognosis. A better understanding of genetic alterations as well as the physiologic and pathophysiologic roles of RAB GTPases may open new opportunities for therapeutic intervention and better outcomes.
Cloning and mRNA Expression of Human Unconventional Myosin-IC (1994)

The complete deduced amino acid sequence and mRNA expression of human unconventional myosin-IC (HuncM-IC) are described. Sequencing of overlapping cDNA clones reveals a message of 4666 nucleotides with a single open reading frame predicted to encode a 127 kDa protein of 1109 amino acids. HuncM-IC is composed of three discrete regions: a characteristic N-terminal myosin head with predicted actin and ATP-binding sites; a neck domain with an "IQ motif", predicted to bind a single light chain; and a C-terminal tail with a putative membrane-binding site. In addition, the tail contains an src-homology 3 domain. The presence of a single IQ motif and an src-homology 3 domain is reminiscent of "long-tailed" myosins-I from amoeboid organisms, a supposition confirmed by multiple sequence alignment. Northern blot analysis of human tissues shows that HuncM-IC is ubiquitously expressed, with the highest levels in kidney, prostate, colon, liver and ovary. The results show that "amoeboid" myosins-I are not restricted to amoeboid organisms, rather they are expressed in the metazoa as well.
Effect of i. v. and Oral Administration of Heparinoids G31150, G31150-A and of Nitrilotriacetic Acid on Blood Coagulation (1971)

Summary The heparinoid G31150-A and nitrilotriacetic acid (NTA) were compared with heparinoid G31150 and heparin. In vitro the anticoagulant activity of NTA was 1/20000, G31150-A 1/8th and G31150, 1/10th of heparin. On intravenous injection in dogs, the degree and duration of hypocoagulability was about the same for the two heparinoids but less than predicted from the effects in vitro. Injection of 25 mg NTA/kg caused a slight increase in coagulation time lasting over 4 hours. When heparinoid G31150-A and NTA were given orally, there was an increase in clotting time of blood and metachromatic activity appeared in the urine. The greatest amount of activity was excreted with the highest dose of NTA alone. Microelectrophoresis of the urinary concentrate indicated the excreted material after NTA was not heparin, but another mucopolysaccharide; after G31150, a heparinoid. These results were confirmed by the resulting absorption spectrum of the dye obtained with added urinary concentrate. It is suggested that the effects produced when a chelating agent is given with oral heparin and heparinoids may be due to the release of a mucopolysaccharide.
Site-Directed Conjugation of “Clicked” Glycopolymers To Form Glycoprotein Mimics:  Binding to Mammalian Lectin and Induction of Immunological Function (2007)

Synthesis of well-defined neoglycopolymer-protein biohybrid materials and a preliminary study focused on their ability of binding mammalian lectins and inducing immunological function is reported. Crucial intermediates for their preparation are well-defined maleimide-terminated neoglycopolymers (M(n) = 8-30 kDa; M(w)/M(n) = 1.20-1.28) presenting multiple copies of mannose epitope units, obtained by combination of transition-metal-mediated living radical polymerization (TMM LRP) and Huisgen [2+3] cycloaddition. Bovine serum albumin (BSA) was employed as single thiol-containing model protein, and the resulting bioconjugates were purified following two independent protocols and characterized by circular dichroism (CD) spectroscopy, SDS PAGE, and SEC HPLC. The versatility of the synthetic strategy presented in this work was demonstrated by preparing a small library of conjugating glycopolymers that only differ from each other for their relative epitope density were prepared by coclicking of appropriate mixtures of mannopyranoside and galactopyranoside azides to the same polyalkyne scaffold intermediate. Surface plasmon resonance binding studies carried out using recombinant rat mannose-binding lectin (MBL) showed clear and dose-dependent MBL binding to glycopolymer-conjugated BSA. In addition, enzyme-linked immunosorbent assay (ELISA) revealed that the neoglycopolymer-protein materials described in this work possess significantly enhanced capacity to activate complement via the lectin pathway when compared with native unmodified BSA.