gebhart/patrepeval huggingface dataset:
Does the Neuron "Learn" like the Synapse? (1988)

An improved learning paradigm that offers a significant reduction in computation time during the supervised learning phase is described. It is based on extending the role that the neuron plays in artificial neural systems. Prior work has regarded the neuron as a strictly passive, non-linear processing element, and the synapse on the other hand as the primary source of information processing and knowledge retention. In this work, the role of the neuron is extended insofar as allowing its parameters to adaptively participate in the learning phase. The temperature of the sigmoid function is an example of such a parameter. During learning, both the synaptic interconnection weights wijm and the neuronal temperatures Tim are optimized so as to capture the knowledge contained within the training set. The method allows each neuron to possess and update its own characteristic local temperature. This algorithm has been applied to logic type of problems such as the XOR or parity problem, resulting in a significant decrease in the required number of training cycles.
Decision Representation Language (DRL) and Its Support Environment (1989)

In this report, I describe a language, called Decision Representation Language (DRL), for representing the qualitative aspects of decision making processes such as the alternatives being evaluated, goals to satisfy, and the arguments evaluating the alternatives. Once a decision process is represented in this language, the system can provide a set of services that support people making the decision. These services, together with the interface such as the object and the different presentation formats, form the support environment for using the language. I describe the services that have been so far identified to be useful — the managements of dependency, plausibility, viewpoints, and precedents. I also discuss how this work on DRL is related to other studies on decision making.
Comparison of the Dependence of the TD50 on Maximum Tolerated Dose for Mutagens and Nonmutagens (1992)

The relationship between the minimum TD50 (i.e., the TD50 measured at the most sensitive site) and the maximum dose administered (maxD) in rodent carcinogenicity bioassays was investigated separately for mice and rats. The relationship between log(1/TD50) and log(1/maxD) was analyzed as a function of (1) mutagenicity and (2) the statistical significance cutoff for selecting the minimum TD50 values. For rat bioassays, the variance of log(1/TD50) is larger and the correlation of log(1/TD50) with log(1/maxD) is weaker for mutagens than for nonmutagens, suggesting that the relationship between minimum TD50 and MTD is, in general, stronger for nonmutagens than for mutagens. The difference in correlation does not depend on the TD50 statistical significance cutoff, but the difference in variance is not significant for the most stringently selected dataset. For mouse bioassays, no significant mutagen/nonmutagen differences in log(1/TD50) variance are found. A significantly weaker correlation of log(1/TD50) with log(1/maxD) for mutagens in comparison to nonmutagens occurs only for the dataset with minimum TD50 chosen at the least stringent level, suggesting that this difference may be due to chance variation. We also looked for changes in correlation and regression parameters as a function of mutagenic potency in Salmonella; the variance of log(1/TD50) and its correlation with log(1/maxD) are not found to vary in a consistent manner. Taken as a whole, our results indicate that (1) mutagenicity is a determinant of the TD50/maxD relationship in rats and (2) any effect that mutagenicity may exert on the TD50/maxD relationship in mice is unimportant.(ABSTRACT TRUNCATED AT 250 WORDS)
Single‐Doped White Organic Light‐Emitting Device with an External Quantum Efficiency Over 20% (2013)

A white OLED with a maximum EQE of 20.1%, CIE coordinates of (0.33, 0.33) and CRI of 80 is fabricated based on platinum(II) bis(N-methyl-imidazolyl)benzene chloride (Pt-16). The device emission spectrum and the chemical structure of Pt-16 are shown in the inset of the efficiency versus luminance graph.
A fast algorithm to remove decaying DC and harmonics for protective relaying (2004)

In protection relaying schemes of power system, the half-cycle discrete Fourier transform (HCDFT) is most widely used for fast computing the fundamental frequency component. However, when a fault occurs, both the voltage and current signals contain large harmonics and the decaying dc component during the fault duration. These abnormal components involved in measurements extremely postpone the convergent speed of conventional HCDFT algorithm. In order to overcome the above problems, this paper presents a novel algorithm, which combines the constrictive band-pass filter and HCDFT. The proposed algorithm effectively suppresses the abnormal frequency components and the fundamental frequency component can be achieved very fast, which only require half-cycle data window. The performance of this algorithm is proved by a lot of simulations with EMTP.
Analysis of Mouse Oocyte Activation Suggests the Involvement of Sperm Perinuclear Material1 (1998)

The mouse oocyte can be activated by injection of a single, intact mouse spermatozoon or its isolated head. Isolated tails are unable to activate the oocyte. Active sperm-borne oocyte-activating factor(s) (SOAF) appears during transformation of the round spermatid into the spermatozoon. The action of SOAF is not highly species-specific: mouse oocytes are activated by injection of spermatozoa from foreign species, such as the hamster, rabbit, pig, human, and even fish. Some SOAF can be extracted by simple freeze-thawing of (hamster) spermatozoa; additional SOAF is obtained by sequential treatment of spermatozoa with Triton X-100 and SDS. Electron microscopic examination of sperm heads during SOAF extraction suggests that the relatively insoluble SOAF is associated with perinuclear material. When microsurgically injected into oocytes, Triton X-100-treated sperm heads (with perinuclear material, but without any membranes) can activate the oocytes, leading to normal embryonic development. Whereas perinuclear components have been believed to play a purely structural role, these data suggest an additional function for them in oocyte activation.
Organization and cell differentiation in lateral roots of Arabidopsis thaliana (1997)

Lateral root formation in plants involves the stimulation of mature pericycle cells to proliferate and redifferentiate to create a new organ. The simple organization of the root of Arabidopsis thaliana allows the development of lateral root primordia to be characterized histologically. We have divided the process of lateral root development into 8 stages defined by specific anatomical characteristics and cell divisions. To identify the cell types in the developing primordium we have generated a collection of marker lines that express beta-glucuronidase in a tissue- or cell type-specific manner in the root. Using these tools we have constructed a model describing the lineage of each cell type in the lateral root. These studies show that organization and cell differentiation in the lateral root primordia precede the appearance of a lateral root meristem, with differential gene expression apparent after the first set of divisions of the pericycle.
Conformations of intrinsically disordered proteins are influenced by linear sequence distributions of oppositely charged residues (2013)

The functions of intrinsically disordered proteins (IDPs) are governed by relationships between information encoded in their amino acid sequences and the ensembles of conformations that they sample as autonomous units. Most IDPs are polyampholytes, with sequences that include both positively and negatively charged residues. Accordingly, we focus here on the sequence-ensemble relationships of polyampholytic IDPs. The fraction of charged residues discriminates between weak and strong polyampholytes. Using atomistic simulations, we show that weak polyampholytes form globules, whereas the conformational preferences of strong polyampholytes are determined by a combination of fraction of charged residues values and the linear sequence distributions of oppositely charged residues. We quantify the latter using a patterning parameter κ that lies between zero and one. The value of κ is low for well-mixed sequences, and in these sequences, intrachain electrostatic repulsions and attractions are counterbalanced, leading to the unmasking of preferences for conformations that resemble either self-avoiding random walks or generic Flory random coils. Segregation of oppositely charged residues within linear sequences leads to high κ-values and preferences for hairpin-like conformations caused by long-range electrostatic attractions induced by conformational fluctuations. We propose a scaling theory to explain the sequence-encoded conformational properties of strong polyampholytes. We show that naturally occurring strong polyampholytes have low κ-values, and this feature implies a selection for random coil ensembles. The design of sequences with different κ-values demonstrably alters the conformational preferences of polyampholytic IDPs, and this ability could become a useful tool for enabling direct inquiries into connections between sequence-ensemble relationships and functions of IDPs.
A Factor VIII Minigene Comprising the Truncated Intron I of Factor IX Highly Improves the In Vitro Production of Factor VIII (2001)

The biosynthesis of coagulation factor VIII (FVIII) is hampered by successive controls that limit its production. To improve this production, a truncated intron I sequence of factor IX (TFIXI1) was inserted in FVIII cDNA in place of FVIII introns 1, 12 and 13 and also as a combination between introns 1 and 12, and introns 1 and 13. The intron 12 and 13 locations were targeted because this region was previously shown to contain a transcriptional silencer. The expression of FVIII in CHO and HepG2 cells revealed important variations in the properties of the minigenes depending on the TFIXI1 insertion sites. In FVIII intron 13 location the TFIXI1 seemed to diminish the transcriptional silencer activity, whereas it was poorly spliced in intron 12 position. Among the five constructs, FVIII I1+13 leaded to a significant improvement in FVIII secretion (13 times) that was associated with a dramatic intracellular accumulation in cells. Therefore, the FVIII I1+13 minigene could represent a particular interest to produce recombinant FVIII in vitro as well as in the aim of gene therapy of haemophilia A.
Growth-associated glycosylation of transferrin secreted by HepG2 cells. (1992)

Growth-associated alterations in the oligosaccharide structures of transferrin secreted by HepG2 cells were examined by concanavalin A-crossed affinoimmuno-electrophoresis. Slowly dividing, confluent cultures produced transferrin with a approximately 4.5-fold greater proportion of biantennary complex-type oligosaccharides than rapidly dividing, subconfluent cultures. The activity of N-acetylglucosaminyltransferase V (GlcNAc-T V) and galactosyltransferase were measured in cell extracts from subconfluent and confluent cultures. While the activity of galactosyltransferase remained relatively constant, the activity of GlcNAc-T V was approximately 3.2-fold lower in confluent cultures than in subconfluent cultures. These results suggest that the growth-associated alteration in the oligosaccharides of transferrin is due, at least in part, to the regulation of GlcNAc-T V activity.
DNA Damage Response Inhibitor Combinations Exert Synergistic Antitumor Activity in Aggressive B-Cell Lymphomas (2019)

The DNA damage response (DDR) kinases ATR, Chk1, and Wee1 play vital roles in the response to replication stress and in maintaining cancer genomic stability. Inhibitors of these kinases are currently under clinical investigation. Mantle cell lymphoma (MCL) and diffuse large B-cell lymphoma (DLBCL) are aggressive lymphomas whose clinical outcome is still largely unsatisfactory. These cell lymphoma subtypes are highly dependent on both Chk1 and Wee1 for survival. We investigated the activity of the ATR inhibitor AZD6738 as single agent and in combination with either Chk1 (AZD6738) or Wee1 (AZD1775) inhibitors in several preclinical models of MCL and DLBCL. This study included preclinical <i>in vitro</i> activity screening on a large panel of cell lines, both as single agent and in combination, and validation experiments on <i>in vivo</i> models. Cellular and molecular mechanisms of the observed synergistic effect as well as pharmacodynamic analysis of <i>in vivo</i> samples were studied. AZD6738 exerted a strong synergistic cytotoxic effect in combination with both AZD7762 and AZD1775 in the 2 lymphoma subtypes regardless of their <i>TP53, MYC</i>, and <i>ATM</i> mutational status. These DDR inhibitor combinations, similarly to the Chk1/Wee1 inhibitor combination, caused a marked S-phase delay, with an increase in cyclin-dependent kinases (CDK) activity, increased DNA damage, and decreases in Wee1, MYC, and RRM2 protein levels. The synergistic <i>in vitro</i> activity translated to striking <i>in vivo</i> antitumor activity. DDR-DDR inhibitor combinations could potentially offer promising novel therapeutic strategies for patients with B-cell lymphoma.
Arthroscopic Shoulder Stabilization With Suture Anchors: Technique, Technology, and Pitfalls (2001)

Arthroscopic treatment of anterior shoulder instability has evolved significantly during the past decade. Currently, most techniques include the use of suture and suture anchors. A successful outcome is highly dependent on accurate patient selection. Preoperative evaluation, examination with the patient under anesthesia, and defining the pathoanatomy by a thorough arthroscopic examination determine the most effective treatment strategy. Technical skills include the surgeon's ability to accomplish anchor placement, suture passage, and arthroscopic knot tying. Various instruments and techniques are available to facilitate arthroscopic reconstruction. In properly selected patients and with good surgical technique, outcomes should approximate or exceed traditional open stabilization techniques.
NanoBRET—A Novel BRET Platform for the Analysis of Protein–Protein Interactions (2015)

Dynamic interactions between proteins comprise a key mechanism for temporal control of cellular function and thus hold promise for development of novel drug therapies. It remains technically challenging, however, to quantitatively characterize these interactions within the biologically relevant context of living cells. Although, bioluminescence resonance energy transfer (BRET) has often been used for this purpose, its general applicability has been hindered by limited sensitivity and dynamic range. We have addressed this by combining an extremely bright luciferase (Nanoluc) with a means for tagging intracellular proteins with a long-wavelength fluorophore (HaloTag). The small size (19 kDa), high emission intensity, and relatively narrow spectrum (460 nm peak intensity) make Nanoluc luciferase well suited as an energy donor. By selecting an efficient red-emitting fluorophore (635 nm peak intensity) for attachment onto the HaloTag, an overall spectral separation exceeding 175 nm was achieved. This combination of greater light intensity with improved spectral resolution results in substantially increased detection sensitivity and dynamic range over current BRET technologies. Enhanced performance is demonstrated using several established model systems, as well as the ability to image BRET in individual cells. The capabilities are further exhibited in a novel assay developed for analyzing the interactions of bromodomain proteins with chromatin in living cells.
Workplace Wellness Programs Can Generate Savings (2010)

With health care expenditures soaring, there is increasing interest in workplace-based disease prevention and health promotion as a means of improving health while lowering costs. We conduct a critical meta-analysis of the literature on costs and savings associated such programs, focusing on studies with particularly rigorous methods and examining effects on health care costs and absenteeism. We find that medical costs fall about $3.27 for every dollar spent on wellness programs, and absentee day costs fall by about $2.73 for every dollar spent. This average return on investment suggests that the wider adoption of such programs could prove beneficial for budgets and productivity as well as health outcomes.
Electroplating Cu fillings for through-vias for three-dimensional chip stacking (2003)

Three-dimensional (3D) LSI chip stacking with through chip electrodes can realize high-density packaging and high-speed operation performance because the through chip electrode offers the shortest interconnection between stacked chips. The through chip via size we studied was 10 /spl mu/m-square and 70 /spl mu/m-deep. The void free plating is necessary to avoid problems caused by acid solutions remaining in voids. In this paper, systematic studies of the dependence of electroplating conditions on via filling are described. We found that vias could be almost filled completely.
The Immune Response: Targets for the Treatment of Severe Sepsis (2012)

The clinical process of severe sepsis is characterized by extreme inflammation interlinked with potent stimulation of the coagulation cascade often followed by a state of relative immune paralysis. In this paper, we will review many of the potential therapies directed at various steps along the inflammatory cascade from modulation of inflammatory mediators eliciting the immune response, alteration of the host's immune response in both a stimulatory and depressive manner, and taming the overexuberant coagulation response triggered by the fierce coagulation-inflammation cycle. Finally, we will discuss further opportunities for research to improve our ability to design effective therapies.
ChemInform Abstract: The Regioselective Preparation of 1,3‐Diketones. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
[Variations in the plasma concentration of ornithine, citrulline and arginine in acute experimental liver failure]. (1981)

In pigs with acute hepatic failure, induced by one stage hepatic devascularization, plasma amino acids pattern changes markedly. Besides an increase in aromatic amino acid and a decrease in branched chain amino acid levels, urea cycle amino acids levels change. The content of ornithine and citrulline is enhanced, while arginine level drops down dramatically within six hours after surgery. These observations suggest that an enrichment of the arginine content of amino acids solutions recently proposed for the therapy of the hepatic encephalopathy might be useful.
The Chicken Lysozyme 5′ Matrix Attachment Region Increases Transcription from a Heterologous Promoter in Heterologous Cells and Dampens Position Effects on the Expression of Transfected Genes (1990)

Matrix attachment regions (MARs) are DNA elements that dissect the genome into topologically separated domains by binding to a chromosomal skeleton. This study explored the putative influence of the MAR located 5' of the chicken lysozyme gene on expression of heterologous genes in heterologous cell systems. Expression of a construct with the chloramphenicol acetyltransferase (CAT) indicator gene controlled by the herpes simplex virus thymidine kinase promoter (TC) and a construct in which the same transcriptional unit is flanked by chicken lysozyme 5' MARs (MTCM) was assayed after stable transfection into rat fibroblasts. Median CAT activity per copy number in MTCM transfectants was elevated approximately 10-fold relative to that in TC transfectants. Total variation in normalized CAT activity decreased from more than 100-fold among TC transfectants to nearly 6-fold among MTCM transfectants. The steady-state level of transcripts and the relative rate of transcription were increased in MTCM transfectants, as shown by S1 nuclease and run-on transcription assays, respectively. The chicken lysozyme 5' MAR thus can confer elevated, less position-dependent expression on a heterologous promoter in cells of a different species by increasing the density of transcribing RNA polymerase molecules. MAR-mediated transcriptional enhancement suggests that MARs are important for gene expression and not just for DNA packaging.
Tracing rays through graded-index media: a new method (1982)

A new method for tracing rays through graded-index media is presented. The method essentially consists of transforming the ray equation into a convenient form and solving the resulting equation using a standard numerical technique. A detailed comparison of this method with existing methods has also been made, and it is shown that for obtaining a desired accuracy this method requires much less computational effort.
Asymmetric reduction of 2-methyl-2-aryloxyacetic acids by Glomerella cingulata. (1984)

In a previous study,^we found that Glomerella cingulata is one of the few molds which possesses the ability to reduce carboxyl-ic acids to carbinols.
Tensegrity I. Cell structure and hierarchical systems biology (2003)

In 1993, a Commentary in this journal described how a simple mechanical model of cell structure based on tensegrity architecture can help to explain how cell shape, movement and cytoskeletal mechanics are controlled, as well as how cells sense and respond to mechanical forces (J. Cell Sci. 104, 613-627). The cellular tensegrity model can now be revisited and placed in context of new advances in our understanding of cell structure, biological networks and mechanoregulation that have been made over the past decade. Recent work provides strong evidence to support the use of tensegrity by cells, and mathematical formulations of the model predict many aspects of cell behavior. In addition, development of the tensegrity theory and its translation into mathematical terms are beginning to allow us to define the relationship between mechanics and biochemistry at the molecular level and to attack the larger problem of biological complexity. Part I of this two-part article covers the evidence for cellular tensegrity at the molecular level and describes how this building system may provide a structural basis for the hierarchical organization of living systems--from molecule to organism. Part II, which focuses on how these structural networks influence information processing networks, appears in the next issue.
Design, Synthesis, and Structure−Activity Relationships of a Series of 3-[2-(1-Benzylpiperidin-4-yl)ethylamino]pyridazine Derivatives as Acetylcholinesterase Inhibitors (2001)

Starting from the 3-[2-(1-benzylpiperidin-4-yl)ethylamino]-6-phenylpyridazine 1, we performed the design, the synthesis, and the structure-activity relationships of a series of pyridazine analogues acting as AChE inhibitors. Structural modifications were achieved on four different parts of compound 1 and led to the following observations: (i) introduction of a lipophilic environment in the C-5 position of the pyridazine ring is favorable for the AChE-inhibitory activity and the AChE/BuChE selectivity; (ii) substitution and various replacements of the C-6 phenyl group are possible and led to equivalent or slightly more active derivatives; (iii) isosteric replacements or modifications of the benzylpiperidine moiety are detrimental to the activity. Among all derivatives prepared, the indenopyridazine derivative 4g was found to be the more potent inhibitor with an IC(50) of 10 nM on electric eel AChE. Compared to compound 1, this represents a 12-fold increase in potency. Moreover, 3-[2-(1-benzylpiperidin-4-yl)ethylamino]-5-methyl-6-phenylpyridazine 4c, which showed an IC(50) of 21 nM, is 100-times more selective for human AChE (human BuChE/AChE ratio of 24) than the reference compound tacrine.