gebhart/patrepeval huggingface dataset:
The Therapeutic Potential of Host-Defense Antimicrobial Peptides (2002)

Species across the evolutionary scale from insects to mammals deploy peptides to counter microbial invaders. Although, there are considerable differences in the primary and secondary structures of these peptides, they exert their antimicrobial activity by common mechanisms ie. by membrane permeabilization. Extensive structure-function studies have been carried out on this class of antimicrobial peptides and 'designer' peptides have been generated which possess specific antimicrobial activity. In this article the physico-chemical properties and biological activities of host-defense peptides and 'designer' peptides would be reviewed with a view to explore whether they could be used as effective therapeutic agents.
Road shape modelling from digital map data - and implementation of a map supported cruise control (2009)

In recent years, the use of digital map data with road attributes has spread from invehicle navigation systems to more sophisticated driver assistance systems.The data can be used together with a GPS positioning system to provide information about the road network close to the vehicle.The information is sufficient for navigation purposes but for the advanced applications, a more accurate model of the road ahead is needed.This thesis proposes a method for road modelling from commercially available map data using a spline interpolation algorithm.The algorithm has several features, such as a possibility to change the smoothness of the spline and to model intersections and right-hand traffic.The road models can be used in applications where a preview of the road shape is of interest.An example application is also developed; a map supported cruise control.The map supported cruise control uses speed limits and road curvature calculated from the model to automatically adjust the target speed of the cruise control before sharp turns and changes in speed limits.A vehicle model is used to predict the deceleration, which in turn is used to decide when to lower the target speed.The road models are verified against collected GPS-data and it is shown that the models provide better fit to road shape and curvature, compared with the source map data.The improvement is most obvious in sharp curves and intersections.The models were also implemented in a test truck.On-road tests enabled comparison of the driver's speed with the recommended maximum speed calculated from the road models.The tests showed that the developed models are accurate enough to be used for automatic speed adaption.The on-road tests also showed that the map database environment used has to be improved.For example, speed limits are often missing in the map data and errors were found in the position mapping.The map supported cruise control worked well in the few test drives conducted.To improve and expand its use the brakes of the truck must be utilised.Unfortunately, this was not achieved in this study.The final conclusion is that the concept, or a simplified version of it, is promising and might be considered as a feature in future trucks.
A novel active holographic arrayed-waveguide grating for WDM subsystems (2002)

We describe simulation results of a novel active AWG indicating optical add/drop multiplexing with passband-flattened channels and suppressions of 15 dB and EDFA gain equalisation with a dynamic range of 12 dB, based on holographic Fourier techniques.
Original insights on thrombospondin-1-related antireceptor strategies in cancer (2015)

Thrombospondin-1 (TSP-1) is a large matricellular glycoprotein known to be overexpressed within tumor stroma in several cancer types. While mainly considered as an endogenous angiogenesis inhibitor, TSP-1 exhibits multifaceted functionalities in a tumor context depending both on TSP-1 concentration as well as differential receptor expression by cancer cells and on tumor-associated stromal cells. Besides, the complex modular structure of TSP-1 along with the wide variety of its soluble ligands and membrane receptors considerably increases the complexity of therapeutically targeting interactions involving TSP-1 ligation of cell-surface receptors. Despite the pleiotropic nature of TSP-1, many different antireceptor strategies have been developed giving promising results in preclinical models. However, transition to clinical trials often led to nuanced outcomes mainly due to frequent severe adverse effects. In this review, we will first expose the intricate and even sometimes opposite effects of TSP-1-related signaling on tumor progression by paying particular attention to modulation of angiogenesis and tumor immunity. Then, we will provide an overview of current developments and prospects by focusing particularly on the cell-surface molecules CD47 and CD36 that function as TSP-1 receptors; including antibody-based approaches, therapeutic gene modulation and the use of peptidomimetics. Finally, we will discuss original approaches specifically targeting TSP-1 domains, as well as innovative combination strategies with a view to producing an overall anticancer response.
Physiological Changes in Dehydroepiandrosterone Are Not Reflected by Serum Levels of Active Androgens and Estrogens But of Their Metabolites: Intracrinology (1997)

This study analyzes in detail the serum concentration of the active androgens and estrogens, as well as a series of free and conjugated forms of their precursors and metabolites, after daily application for 2 weeks of 10 mL 20% dehydroepiandrosterone (DHEA) solution on the skin to avoid first passage through the liver. In men, DHEA administration caused 175%, 90%, 200% and 120% increases in the circulating levels of DHEA and its sulfate (DHEA-S), DHEA-fatty acid esters, and androst-5-ene-3 beta,17 beta-diol, respectively, with a return to basal values 7 days after cessation of the 14-day treatment. Serum androstenedione increased by approximately 80%, whereas serum testosterone and dihydrotestosterone (DHT) remained unchanged. In parallel with the changes in serum DHEA, the concentrations of the conjugated metabolites of DHT, namely androsterone glucuronide, androstane-3 alpha,17 beta-diol-G, and androstane-3 beta,17 beta-diol-G increased by about 75%, 50%, and 75%, respectively, whereas androsterone-sulfate increased 115%. No consistent change was observed in serum estrone (E1) or estradiol (E2) in men receiving DHEA, whereas serum E1-sulfate and E2-sulfate were slightly and inconsistently increased by about 20%, and serum cortisol and aldosterone concentrations were unaffected by DHEA administration. Almost superimposable results were obtained in women for most steroids except testosterone, which was about 50% increased during DHEA treatment. This increase corresponded to about 0.8 nM testosterone, an effect undetectable in men because they already have much higher (approximately 15 nM) basal testosterone levels. In women, the serum levels of the conjugated metabolites of DHT, namely androsterone glucuronide, androstane-3 alpha,17 beta-diol-G, androstane-3 beta,17 beta-diol-G, and androsterone-sulfate were increased by 125%, 140%, 120% and 150%, respectively. The present study demonstrates that the serum concentrations of testosterone, DHT, E1, and E2 are poor indicators of total androgenic and estrogenic activity. However, the esterified metabolites of DHT appear as reliable markers of the total androgen pool, because they directly reflect the intracrine formation of androgens in the tissues possessing the steroidogenic enzymes required to transform the inactive precursors DHEA and DHEA-S into DHT. As well demonstrated in women, who synthesize almost all their androgens from DHEA and DHEA-S, supplementation with physiological amounts of exogeneous DHEA permits the biosynthesis of androgens limited to the appropriate target tissues without leakage of significant amounts of active androgens into the circulation. This local or intracrine biosynthesis and action of androgens eliminates the inappropriate exposure of other tissues to androgens and thus minimizes the risks of undesirable masculinizing or other androgen-related side effects of DHEA.
PTEN Protects p53 from Mdm2 and Sensitizes Cancer Cells to Chemotherapy (2002)

The PTEN tumor suppressor protein inhibits phosphatidylinositol 3-kinase (PI3K)/Akt signaling that promotes translocation of Mdm2 into the nucleus. When restricted to the cytoplasm, Mdm2 is degraded. The ability of PTEN to inhibit the nuclear entry of Mdm2 increases the cellular content and transactivation of the p53 tumor suppressor protein. Retroviral transduction of PTEN into U87MG (PTEN null) glioblastoma cells increases p53 activity and expression of p53 target genes and induces cell cycle arrest. U87MG/PTEN glioblastoma cells are more sensitive than U87MG/PTEN null cells to death induced by etoposide, a chemotherapeutic agent that induces DNA damage. Previously, tumor suppressor proteins have been supposed to act individually to suppress cancers. Our results establish a direct connection between the activities of two major tumor suppressors and show that they act together to respond to stresses and malignancies. PTEN protects p53 from survival signals, permitting p53 to function as a guardian of the genome. By virtue of its capacity to protect p53, PTEN can sensitize tumor cells to chemotherapy that relies on p53 activity. p53 induces PTEN gene expression, and here it is shown that PTEN protects p53, indicating that a positive feedback loop may amplify the cellular response to stress, damage, and cancer.
Diversity and feasibility of direct bonding: a survey of a dedicated optical technology (1994)

The aim of this paper is to review almost a decade of direct-bonding activities at Philips Research including the diversity and feasibility of direct bonding. The bondability of a material is determined by its geometrical shape and mechanical, physical, and chemical surface states. Physically direct bonding provides a vacuumtight bond, which is jointless and glueless, and it permits engineering of the interfaces to be bonded. Layers can be buried, and reflective-lossless bonds between optical elements can be created. A variety of materials are investigated: (refractory) metals, a semimetal, boron, diamond, a carbide, fluorides, nitrides, oxides, and a chalcogenide. The applications that we describe relate to interface engineering, waveguiding, and the direct bonding of a fiber plate.
Fabrication of Tissue Engineered Tympanic Membrane Patches Using Computer‐Aided Design and Injection Molding (2004)

The use of computer-aided design and injection molding technologies allows for the fabrication of very small, precisely shaped chondrocyte-seeded calcium alginate structures that faithfully maintain their shape during in vitro culture. In vitro fabrication of tympanic membrane patches with a precisely controlled geometry may have the potential to provide a minimally invasive alternative to traditional methods for the repair of chronic tympanic membrane perforations.
Jaw-Tongue Reflex: Afferents, Central Pathways, and Synaptic Potentials in Hypoglossal Motoneurons in the Cat (2000)

The tongue position is reflexively controlled by the jaw position (the jaw-tongue reflex). The purpose of this study was to clarify the mechanism of this reflex in terms of afferents, central pathways, and synaptic potentials in hypoglossal motoneurons in the cat. Intracellular recordings from hypoglossal motoneurons revealed that electrical stimulation of the temporalis muscle nerve evoked excitatory and inhibitory post-synaptic potentials in hypoglossal motoneurons. The threshold of temporalis muscle nerve stimulation for evoking the synaptic potentials was higher than 2.0 times the nerve threshold. The amplitude of the potentials increased with stimulus intensity up to 5.0 times the nerve threshold. Punctate light pressure applied to the temporalis muscle induced a tonic depolarizing potential in hypoglossal motoneurons on which action potentials as well as depolarizing synaptic activation noise were superimposed. On the other hand, electrical stimulation of the temporalis muscle during jaw-opening could slightly inhibit the electromyographic activities in the genioglossus and styloglossus muscles. Lesions including the Probst's tract at the level caudal to the trigeminal motor nucleus abolished both excitation and inhibition in hypoglossal motoneurons induced by tonic depression of the lower jaw, but exerted no effects on either the tonic stretch reflex or the trigemino-hypoglossal reflex. In contrast, lesions including the trigeminal spinal tract produced no changes in either excitation or inhibition of hypoglossal motoneurons induced by temporalis muscle afferents, whereas the excitation of hypoglossal motoneurons was abolished by the lesions. We conclude that the group II muscle spindle afferents from the temporalis muscle are primarily responsible for evoking the jaw-tongue reflex.
Gait Analysis Using a Shoe-Integrated Wireless Sensor System (2008)

We describe a wireless wearable system that was developed to provide quantitative gait analysis outside the confines of the traditional motion laboratory. The sensor suite includes three orthogonal accelerometers, three orthogonal gyroscopes, four force sensors, two bidirectional bend sensors, two dynamic pressure sensors, as well as electric field height sensors. The "GaitShoe" was built to be worn in any shoe, without interfering with gait and was designed to collect data unobtrusively, in any environment, and over long periods. The calibrated sensor outputs were analyzed and validated with results obtained simultaneously from the Massachusetts General Hospital, Biomotion Laboratory. The GaitShoe proved highly capable of detecting heel-strike and toe-off, as well as estimating foot orientation and position, inter alia.
A uniform extracellular stimulus triggers distinct cAMP signals in different compartments of a simple cell (2001)

cAMP, the classical second messenger, regulates many diverse cellular functions. The primary effector of cAMP signals, protein kinase A, differentially phosphorylates hundreds of cellular targets. Little is known, however, about the spatial and temporal nature of cAMP signals and their information content. Thus, it is largely unclear how cAMP, in response to different stimuli, orchestrates such a wide variety of cellular responses. Previously, we presented evidence that cAMP is produced in subcellular compartments near the plasma membrane, and that diffusion of cAMP from these compartments to the bulk cytosol is hindered. Here we report that a uniform extracellular stimulus initiates distinct cAMP signals within different cellular compartments. By using cyclic nucleotide-gated ion channels engineered as cAMP biosensors, we found that prostaglandin E(1) stimulation of human embryonic kidney cells caused a transient increase in cAMP concentration near the membrane. Interestingly, in the same time frame, the total cellular cAMP rose to a steady level. The decline in cAMP levels near the membrane was prevented by pretreatment with phosphodiesterase inhibitors. These data demonstrate that spatially and temporally distinct cAMP signals can coexist within simple cells.
β-Hairpin stabilization through an interstrand triazole bridge (2011)

β-Hairpin peptides were conformationally stabilized through a 1,4 disubstituted 1,2,3-triazole interstrand linkage. A NMR conformational analysis revealed that the β-hairpin content depends on the number and position of substituent methylene units of the 1,2,3-triazole ring. These results will allow the design of metabolically stable peptidomimetic analogs of bioactive β-hairpin peptides.
Ethernet aggregation and transport infrastructure OAM and protection issues (2009)

As Ethernet traffic increases rapidly in carrier networks, service providers have an increasing need for Ethernet infrastructure networking in order to scale their Ethernet services. Ethernet infrastructure transports Ethernet traffic over distance, protects the traffic from link and nodal failures, and aggregates Ethernet connections from a large number of low-speed ports onto a much smaller number of high-speed ports. An Ethernet infrastructure allows service providers to inexpensively interconnect end user locations with one another for private line services, and to interconnect end users with the VPLS and IP/MPLS service edges for enterprise, residential, and mobility services. OAM and protection capabilities are critical to enable Ethernet to be deployed as an infrastructure technology. This article reviews several important developments in Ethernet OAM and protection standards, and discusses how those capabilities are vital for the creation of an effective Ethernet infrastructure.
Enhanced GMR ratio of dual spin valve with monolayer pinned structure (2006)

A new type of dual spin valve (DSV) structure with enhanced giant magnetoresistance (GMR) ratio is proposed, and the performance is characterized by the prototype read heads. The bottom part of DSV is kept as a synthetic pinned structure and only the top part adopts a monolayer pinned structure. The top monolayer pin DSV (TM-DSV) showed three percentage points higher GMR ratio and 20% higher /spl Delta/R value. After the mechanical lapping process, the monolayer pinned layer can be stabilized by not only sense current but also large stress induced anisotropy due to Villari effect. After the quantitative analysis, the induced stress is measured to be about 4.2/spl times/10/sup 9/ N/m/sup 2/. The bias point was tuned by adjustment of Cu spacer layer thickness using the RKKY interaction between pinned layer and free layer. There is no reliability concern related to the monolayer pinned structure. In a perpendicular magnetic recording system, TM-DSV technology could successfully demonstrate the output of 35.5 mV//spl mu/m and /spl sim/170 Gbits/in/sup 2/ density feasibility.
A taxonomy of web search (2002)

Classic IR (information retrieval) is inherently predicated on users searching for information, the so-called "information need". But the need behind a web search is often not informational -- it might be navigational (give me the url of the site I want to reach) or transactional (show me sites where I can perform a certain transaction, e.g. shop, download a file, or find a map). We explore this taxonomy of web searches and discuss how global search engines evolved to deal with web-specific needs.
Detecting Word Substitutions in Text (2008)

Searching for words on a watchlist is one way in which large-scale surveillance of communication can be done, for example in intelligence and counterterrorism settings.One obvious defense is to replace words that might attract attention to a message with other, more innocuous, words.For example, the sentence "the attack will be tomorrow" might be altered to "the complex will be tomorrow", since 'complex' is a word whose frequency is close to that of 'attack'.Such substitutions are readily detectable by humans since they do not make sense.We address the problem of detecting such substitutions automatically, by looking for discrepancies between words and their contexts, and using only syntactic information.We define a set of measures, each of which is quite weak, but which together produce per-sentence detection rates around 90% with false positive rates around 10%.Rules for combining persentence detection into per-message detection can reduce the false positive and false negative rates for messages to practical levels.We test the approach using sentences from the Enron email and Brown corpora, representing informal and formal text respectively.
Protein Electron Transfer Rates Set by the Bridging Secondary and Tertiary Structure (1991)

The rate of long-distance electron transfer in proteins rapidly decreases with distance, which is indicative of an electron tunneling process. Calculations predict that the distance dependence of electron transfer in native proteins is controlled by the protein's structural motif. The helix and sheet content of a protein and the tertiary arrangement of these secondary structural units define the distance dependence of electronic coupling in that protein. The calculations use a tunneling pathway model applied previously with success to ruthenated proteins. The analysis ranks the average distance decay constant for electronic coupling in electron transfer proteins and identifies the amino acids that are coupled to the charge localization site more strongly or weakly than average for their distance.
In Vitro Effector Activity ofPneumocystis murina-Specific T-Cytotoxic-1 CD8+T Cells: Role of Granulocyte-Macrophage Colony-Stimulating Factor (2005)

Human immunodeficiency virus (HIV)-related opportunistic infections continue to occur in patients who are newly diagnosed with HIV infection, those in the early course of highly active antiretroviral therapy or nonadherent to HIV care, and other immunosuppressed individuals. One of the most common opportunistic infections in these patients is Pneumocystis pneumonia. CD8+ T cells are recruited to the lung after P. carinii infection and have been associated with both lung injury and host defense. This variability may be due to subpopulations of CD8+ T cells recruited to the lung. We have previously shown using adoptive transfer studies that in vivo-generated T-cytotoxic-1 (Tc1) CD8+ T cells, defined by the secretion of gamma interferon (IFN-gamma), have effector activity against Pneumocystis spp. in vitro as well as in vivo. To better understand the mechanisms of these effects, we generated, expanded, and tested Tc1 and Tc2 CD8+ T cells specific for P. murina ex vivo. Tc1-polarized CD8+ T cells secreted higher levels of IFN-gamma and granulocyte-macrophage colony-stimulating factor (GM-CSF) and lower levels of interleukin-4 (IL-4), IL-5, IL-10, and IL-13 than Tc2 CD8+ T cells when stimulated with P. murina antigen. Moreover, Tc1 CD8+ T cells demonstrated enhanced effector activity in a macrophage-mediated killing assay which was independent of cell contact. The augmentation in macrophage-mediated P. murina killing was significantly abrogated when GM-CSF was neutralized in the Tc1 CD8+ T cells. These data support the possibility that antigen-specific GM-CSF secretion is critical for effector activity of P. murina-specific Tc1 CD8+ T cells in vitro.
Angiogenesis and breast cancer. (1994)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1994.12.3.441 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 7509850 Angiogenesis and breast cancer. J FolkmanxJ FolkmanSearch for articles by this author https://doi.org/10.1200/JCO.1994.12.3.441 First Page PDF © 1994 by American Society of Clinical Oncology