gebhart/patrepeval huggingface dataset:
Convolutionally coded DS/CDMA system using multi-antenna transmission (2002)

In this paper, we discuss the use of convolutional codes with a multi-antenna transmission scheme for DS/CDMA systems. The binary input data to a rate 1/M encoder produces M coded bits, which, in turn, are assigned to M different antennas and transmitted from each antenna simultaneously. An intentional delay of several chips duration is introduced at each antenna before transmission, which enables a receiver to distinguish the signals from different antennas. Because the proposed scheme utilizes spatial and time domains for coding, it can achieve not only implicit time-diversity through the use of coding with interleaving, but also space-diversity through the transmission from multiple antennas. Multi-antenna schemes with convolutional codes can perform better than conventional single antenna schemes with the same codes and transmission diversity technique with the same number of transmitting antennas, especially when a fading is relatively slow and interleaving size is limited.
Attempts to Deal with the "Crisis in Cataloging" at the Library of Congress in the 1940s (1987)

This article provides a review of the ten-year period at the Library of Congress (LC) during which attempts to deal with the "crisis in cataloging" set the stage for the profound changes in Anglo-American cataloging theory and rules that occurred during the 1950s and 1960s. The review centers around the previously unpublished report of the Librarian's Committee, which recommended reorganization, simplified cataloging, cooperative cataloging, changes in personnel policies, and changes in methods of cost analysis. Each of these recommendations is examined in detail, and the results of implementation are traced through the decade that followed.
Human cytomegalovirus vaccines (2007)

Efforts to develop a human cytomegalovirus (HCMV) vaccine began more than 30 years ago in response to then recent reports that HCMV was capable of causing severe congenital disease. During the intervening years, our understanding of HCMV biology and immunology has increased dramatically. That knowledge, coupled with the introduction of several new vaccine methodologies, opened the door to an impressive expansion of HCMV vaccine research, particularly during the past decade. This chapter focuses on the principles underlying HCMV vaccine development and on the vaccine approaches that are currently under investigation.
The Escherichia coli DNA polymerase III holoenzyme contains both products of the dnaX gene, tau and gamma, but only tau is essential (1993)

The replicative polymerase of Escherichia coli, DNA polymerase III, consists of a three-subunit core polymerase plus seven accessory subunits. Of these seven, tau and gamma are products of one replication gene, dnaX. The shorter gamma is created from within the tau reading frame by a programmed ribosomal -1 frameshift over codons 428 and 429 followed by a stop codon in the new frame. Two temperature-sensitive mutations are available in dnaX. The 2016(Ts) mutation altered both tau and gamma by changing codon 118 from glycine to aspartate; the 36(Ts) mutation affected the activity only of tau because it altered codon 601 (from glutamate to lysine). Evidence which indicates that, of these two proteins, only the longer tau is essential includes the following. (i) The 36(Ts) mutation is a temperature-sensitive lethal allele, and overproduction of wild-type gamma cannot restore its growth. (ii) An allele which produced tau only could be substituted for the wild-type chromosomal gene, but a gamma-only allele could not substitute for the wild-type dnaX in the haploid state. Thus, the shorter subunit gamma is not essential, suggesting that tau can be substitute for the usual function(s) of gamma. Consistent with these results, we found that a functional polymerase was assembled from nine pure subunits in the absence of the gamma subunit. However, the possibility that, in cells growing without gamma, proteolysis of tau to form a gamma-like product in amounts below the Western blot (immunoblot) sensitivity level cannot be excluded.
Longitudinal and transverse components of excitons in a spherical quantum dot (2000)

Exciton states confined in a spherical quantum dot (QD) are studied in a weak confinement regime with the consideration of the electron-hole exchange interaction and induced surface charge density. Except for special cases, most exciton states are longitudinal (L)--transverse (T) mixed modes. With an increase of radius, the LT mixed modes approach bulk L, T, and surface (S) modes. When the energies of LT mixed modes get close to that of the S mode, they acquire considerable amount of S-mode character. It is demonstrated that the effect of the surface charge density does not affect the L-mode exciton confined in an arbitrary shape. Within the long-wavelength approximation, one-photon transitions toward the pure L and T modes are forbidden, and for other states the oscillator strengths per unit volume become maximum around the energy of the S mode.
Hematopoietic and Nature Killer Cell Development from Human Pluripotent Stem Cells (2013)

Natural killer (NK) cells are key effectors of the innate immune system, protecting the host from a variety of infections, as well as malignant cells. Recent advances in the field of NK cell biology have led to a better understanding of how NK cells develop. This progress has directly translated to improved outcomes in patients receiving hematopoietic stem cell transplants to treat potentially lethal malignancies. However, key differences between mouse and human NK cell development and biology limits the use of rodents to attain a more in depth understanding of NK cell development. Therefore, a readily accessible and genetically tractable cell source to study human NK cell development is warranted. Our lab has pioneered the development of lymphocytes, specifically NK cells, from human embryonic stem cells (hESCs) and more recently induced pluripotent stem cells (iPSCs). This chapter describes a reliable method to generate NK cells from hESCs and iPSCs using murine stromal cell lines. Additionally, we include an updated approach using a spin-embryoid body (spin-EB) differentiation system that allows for human NK cell development completely defined in vitro conditions.
Genetic Identification of Residues Involved in Association of α and β G-Protein Subunits† (1994)

The GPA1, STE4, and STE18 genes of Saccharomyces cerevisiae encode the alpha, beta, and gamma subunits, respectively, of a G protein involved in the mating response pathway. We have found that mutations G124D, W136G, W136R, and delta L138 and double mutations W136R L138F and W136G S151C of the Ste4 protein cause constitutive activation of the signaling pathway. The W136R L138F and W136G S151C mutant Ste4 proteins were tested in the two-hybrid protein association assay and found to be defective in association with the Gpa1 protein. A mutation at position E307 of the Gpa1 protein both suppresses the constitutive signaling phenotype of some mutant Ste4 proteins and allows the mutant alpha subunit to physically associate with a specific mutant G beta subunit. The mutation in the Gpa1 protein is adjacent to the hinge, or switch, region that is required for the conformational change which triggers subunit dissociation, but the mutation does not affect the interaction of the alpha subunit with the wild-type beta subunit. Yeast cells constructed to contain only the mutant alpha and beta subunits mate and respond to pheromones, although they exhibit partial induction of the pheromone response pathway. Because the ability of the modified G alpha subunit to suppress the Ste4 mutations is allele specific, it is likely that the residues defined by this analysis play a direct role in G-protein subunit association.
Optical waveguides in GaAs–AlGaAs epitaxial layers (1973)

Optical transmission properties are described for two types of waveguides formed in AlxGa1−xAs–GaAs–Alx Ga1−xAs epitaxial layers. The first consists of long smooth-walled mesas formed by a masking and etching procedure and the second are obtained using an additional etching step to selectively etch the GaAs layer. The latter structure is potentially useful in forming active devices such as modulators since this structure is self-masking for contacting of the top layer by conventional evaporation techniques. The waveguide dimensions are typically 1–30 μm wide, [sine wave] 1 μm thick, and several mm in length. The transmission measurements are quite similar for both types of guides with attenuation as low as [inverted lazy s]2 cm−1 in wide ([inverted lazy s]20 μm) guides but with losses increasing with decreasing width. The loss appears to arise from imperfections and compositional inhomogeneities in the epitaxial layers.
Three-Dimensional Path Planning for Virtual Bronchoscopy (2004)

Multidetector computed-tomography (MDCT) scanners provide large high-resolution three-dimensional (3-D) images of the chest. MDCT scanning, when used in tandem with bronchoscopy, provides a state-of-the-art approach for lung-cancer assessment. We have been building and validating a lung-cancer assessment system, which enables virtual-bronchoscopic 3-D MDCT image analysis and follow-on image-guided bronchoscopy. A suitable path planning method is needed, however, for using this system. We describe a rapid, robust method for computing a set of 3-D airway-tree paths from MDCT images. The method first defines the skeleton of a given segmented 3-D chest image and then performs a multistage refinement of the skeleton to arrive at a final tree structure. The tree consists of a series of paths and branch structural data, suitable for quantitative airway analysis and smooth virtual navigation. A comparison of the method to a previously devised path-planning approach, using a set of human MDCT images, illustrates the efficacy of the method. Results are also presented for human lung-cancer assessment and the guidance of bronchoscopy.
Direct Gene Transfer by Liposomes (1994)

AbstractCationic liposomes are widely used for the delivery of genes both in vivo and in clinical trials. DC-chol liposome formulation was developed by us for relatively high activity of transfection and low level of toxicity for most cell types. Different strategies are described for achieving regulated transgene expression as well as expression for a prolonged period of time using DC-chol liposomes.
Spheroid body-forming cells in the human gastric cancer cell line MKN-45 possess cancer stem cell properties (2012)

The cancer stem cell theory hypothesizes that cancer stem cells (CSCs), which possess self-renewal and other stem cell properties, are regarded as the cause of tumor formation, recurrence and metastasis. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs. In this study, we enriched gastric cancer stem cells through spheroid body formation by cultivating the human gastric cancer cell line MKN-45 in defined serum-free medium. The stemness characteristics of spheroid body-forming cells, including self-renewal, proliferation, chemoresistance, tumorigenicity of the MKN-45 spheroid body-forming cells were evaluated, and the expression levels of stemness genes and related proteins in the MKN-45 spheroid body-forming cells were assessed. Furthermore, immunofluorescence staining for the stem cell markers on spheroid body-forming cells was examined to evaluate the association between stemness factors (Oct4, Sox2, Nanog) and the proposed CSC marker CD44. Our data demonstrated that non-adherent spheroid body-forming cells from the gastric cancer cell line MKN-45 cultured in stem cell-conditioned medium possessed gastric CSC properties, such as persistent self-renewal, extensive proliferation, drug resistance, high tumorigenic capacity and overexpression of CSC-related genes and proteins (Oct4, Sox2, Nanog and CD44), compared with the parental cells. More importantly, CD44-positive cells co-expressing the pluripotency genes Oct4, Sox2 and Nanog may represent gastric CSCs. Further experiments using more refined selection criteria such as a combination of two or multiple markers would be useful to specifically identify and purify CSCs.
A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins (2002)

Fragile X syndrome is a common form of inherited mental retardation caused by the loss of FMR1 expression. The FMR1 gene encodes an RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator. In Drosophila, the fly homolog of the FMR1 protein (dFMR1) binds to and represses the translation of an mRNA encoding of the microtuble-associated protein Futsch. We have isolated a dFMR1-associated complex that includes two ribosomal proteins, L5 and L11, along with 5S RNA. The dFMR1 complex also contains Argonaute2 (AGO2) and a Drosophila homolog of p68 RNA helicase (Dmp68). AGO2 is an essential component for the RNA-induced silencing complex (RISC), a sequence-specific nuclease complex that mediates RNA interference (RNAi) in Drosophila. We show that Dmp68 is also required for efficient RNAi. We further show that dFMR1 is associated with Dicer, another essential component of the RNAi pathway, and microRNAs (miRNAs) in vivo, suggesting that dFMR1 is part of the RNAi-related apparatus. Our findings suggest a model in which the RNAi and dFMR1-mediated translational control pathways intersect in Drosophila. Our findings also raise the possibility that defects in an RNAi-related machinery may cause human disease.
Review of Drug Interactions: A Comprehensive Update (2014)

Prescribing multiple medications predisposes to possibilities of occurrence of drug interactions. Various different terminology and ways exist to classify or arrange drug interactions. Drugs interact with other drugs, foods, beverages and herbs; outside or inside the body. Knowledge of In vitro interactions is essential to avoid loss of activity of drugs before administration. Although every theoretical drug interaction may not manifest in practice, drug interaction is a prominent cause of adverse or undesired events related to drug administration. Amongst the herbs, St. John’s wort has a potential of producing significant drug interactions due to its capacity to induce metabolism of number of drugs. In vivo interactions at pharmacokinetic level affect absorption, distribution, biotransformation or excretion of drugs. Induction or inhibition of cytochrome P450 (CYP450) enzymes forms a major basis of drug interactions. Induction of metabolism of a substrate drug leads to treatment failure. Inhibition of metabolism leads to serious interactions by aggravating toxicity of substrate drugs. As compared to induction, inhibition is a fairly rapid process, and number of precipitant drugs which inhibit the metabolism is much more than that of inducers. Role of drug transporters, especially P-glycoprotein (P-gp), in causation of drug interactions is being increasingly identified. P-gp affects absorption, distribution and excretion, and hence plays a major role in pharmacokinetic drug interactions. Additionally, P-gp works hand in hand with CYP450 enzymes. In pharmacodynamic interactions, the drugs synergise or antagonise the effect at the level of target of action. Clinically beneficial and reparative drug interactions are explored to obtain useful drug combinations. Extensive research has led to development of a large number of In vitro and In vivo methods to detect and predict drug interactions. Appropriate awareness and knowledge of possible drug interactions is crucial in prevention of drug interactions and their consequences.
Catabolic Activities of Neisseria meningitidis: Utilization of Succinate (1970)

Glutamate was catabolized at a rapid rate by Neisseria meningitidis, group B. Surprisingly, there was a lag of 5 to 30 min in respiration, but not in CO(2) production from C(1), and an appreciable amount of succinate accumulated. The eventual rapid rate of respiration was not prevented by the addition of chloramphenicol. The lag period was eliminated by combinations of substrates that favored the activity of a glutamate-oxaloacetate transaminase. It is suggested that with glutamate as the sole substrate, the reaction terminated at succinate, required only moderate O(2) uptake, and did not result in the transport of succinate to enzymatic sites. The lag period represented the time required for the accumulation of succinate and its transport to enzymatic sites by energy provided by the metabolism of the remaining glutamate. When the transaminase was operative, on the other hand, successive products of the reaction were immediately placed in contact with enzymatic sites.
Geometry-Adaptive Block Partitioning for Video Coding (2007)

Frame partitioning is a process of key importance in efficient video coding. Most recent video compression technologies, like H.264/AVC, use tree based frame partition. This reveals to be more efficient than simple uniform block partition, typically used in older video coding standards like MPEG-2 or H.263. However, tree based frame partition still does not code efficiently enough video information, as is unable to capture the geometric structure of 2D data. During last years, several works have been developed, mainly in the domain of still image representation and coding, in order to solve such limitations. An example is the use of wedge partitions. Based on these, in this paper, we study a way to better represent and code 2D video data by taking its 2D geometry into account. Our study is developed as an extension of H.264/AVC. Geometry-adaptive partitions are used to improve intra and inter prediction modes. Results obtained with the investigated method show that both better R-D and visual performance can be achieved.
Leydig Cell Protein Synthesis and Steroidogenesis in Response to Acute Stimulation by Luteinizing Hormone in Rats1 (1998)

We examined the temporal relationship between protein synthesis and testosterone production by rat Leydig cells in primary culture. Leydig cells were isolated from adult control Sprague-Dawley rats and from rats that had received LH-suppressive testosterone and estradiol (TE) implants in vivo for 10 days. The cells were incubated for 1-4 h with [35S]methionine in the presence or absence of maximally stimulating ovine LH, and newly synthesized proteins were examined by two-dimensional PAGE autoradiography. Approximately 800-900 newly synthesized polypeptides were readily visible on all autoradiograms, most of which did not differ in the cells from intact control and TE-treated rats. Incubation of cells from the control and treated rats with maximally stimulating LH for 4 h in both cases resulted in significant increases in testosterone production and in three newly synthesized polypeptides. These polypeptides, along with two others that changed little in response to LH, were similar in apparent molecular mass, 30 kDa, but differed in isoelectric point. Time-course studies revealed a temporal relationship between stimulation of the three 30-kDa proteins and of testosterone production. Western blot analysis identified the 30-kDa proteins as steroidogenic acute regulatory protein (StAR). The results of these studies, for the first time utilizing primary cultures of highly purified, testosterone-producing Leydig cells, provide further correlative evidence of a role for StAR protein in the acute regulation of Leydig cell testosterone biosynthesis by LH.
Coronary artery disease in women (1993)

Background: Numerous studies have shown that higher intakes or higher blood concentrations of carotenes are associated with a lower risk of coronary artery disease (CAD). Given the null results in trials of beta-carotene supplementation, considerable attention has focused on the potential role of other dietary carotenoids in the prevention of CAD. Objective: Our objective was to prospectively examine the relation between dietary intakes of specific carotenoids and risk of CAD in women. Design: In 1984, 73 286 female nurses completed a semiquantitative food-frequency questionnaire that assessed their consumption of carotenoids and various other nutrients. The women were followed for 12 y for the development of incident CAD (nonfatal myocardial infarction and fatal CAD), and dietary information was updated in 1986, 1990, and 1994. Results: During 12 y of follow-up (903 590 person-years), we identified 998 incident cases of CAD. After adjustment for age, smoking, and other CAD risk factors, we observed modest but significant inverse associations between the highest quintiles of intake of beta-carotene and alpha-carotene and risk of CAD but no significant relation with intakes of lutein/zeaxanthin, lycopene, or beta-cryptoxanthin. For women in the highest compared with the respective lowest quintile of intake, the relative risks for beta-carotene and alpha-carotene were 0.74 (95% Cl: 0.59, 0.93) and 0.80 (95% CI: 0.65, 0.99), respectively. The association between the specific carotenoids and CAD risk did not vary significantly by current smoking status. Conclusion: Higher intakes of foods rich in alpha-carotene or beta-carotene are associated with a reduction in risk of CAD.
The Luminous Intensity and Luminaire Efficiency Analysis of White LED as Luminaire types for General Lighting (2004)

본 논문에서는 조명용 고휘도(HB, high brightness) 백색 LED(light emitting diode)램프의 배열 및 여러 가지 등기구 글로브(globe)의 형태에 따른 광도(luminous intensity)값과 기구효율(luminaire efficiency)을 컴퓨터 시뮬레이션으로 수행하여 비교분석 하였다. 즉, LED의 배열은 가로×세로(7개×7개)가 정사각형 형태로 49개를 배열하고, LED 간격은 LED의 발광면 중심부로부터 8.2[㎜], 10.0[㎜], 15.0[㎜]로 각각 3가지로 하였으며, 등기구의 글로브는 LED발광면 중심부에서 30[mm], 50[mm] 떨어진 거리로 구분하였다. 또한 등기구 글로브가 없을 때와 있을 때로 구분하여 있을 때에는 글로브 형태를 볼록 원형과 엠보(embossing)형태로 구분하고 엠보는 8.2[㎜], 5.0[㎜]의 크기에 따른 선택으로 시뮬레이션 27가지로 수행하여 등기구의 글로브 형태에 따른 광도 및 기구효율을 고찰해 봄으로써 앞으로 21세기 디지털조명이라고 일컫는 LED를 사용한 LED 광원(light source) 및 등기구 개발에 많은 도움이 되고자 하였다.
CLAVATA1, a regulator of meristem and flower development in Arabidopsis (1993)

We have investigated the effects on plant development of mutations in the Arabidopsis thaliana CLAVATA1 gene. In clavata1 plants, vegetative, inflorescence and floral meristems are all enlarged relative to wild type. The apical meristem can fasciate in the more severe mutant alleles, and this fasciation can occur prior to the transition to flowering. Flowers of clavata1 plants can have increased numbers of organs in all four whorls, and can also have additional whorls not present in wild-type flowers. Double mutant combinations of clavata1 with agamous, apetala2, apetala3 and pistillata indicate that CLAVATA1 controls the underlying floral meristem structure upon which these homeotic genes act. Double mutant combinations of clavata1 with apetala1 and leafy indicate CLAVATA1 plays a role in establishing and maintaining floral meristem identity, in addition to its role in controlling meristem size. In support of this, RNA expression patterns of AGAMOUS and APETALA1 are altered in clavata1 flowers.
An active transcoding proxy to support mobile web access (2002)

In this paper, we present a proxy based system (MOWSER) to support web browsing from mobile clients over wireless networks. Mowser is a proxy agent between the mobile host and the web server, which performs active transcoding of data on both upstream and downstream traffic to present web information to the mobile user according to the QoS parameters set by the user. Active transcoding is defined as modifying the HTTP stream in situ, and it is entirely transparent to the user. Further, our system does not pose any additional requirements on the mobile user. This is an improvement over other proxy based systems, which only transcode images on the downstream and are mostly not configurable. While developed for mobile users, such a system can actually be useful in any low bandwidth scenario.