gebhart/patrepeval huggingface dataset:
High-Stability Ionic Liquids. A New Class of Stationary Phases for Gas Chromatography (2003)

Room-temperature ionic liquids are a class of non-molecular ionic solvents with low melting points. Their properties have the potential to be especially useful as stationary phases in gas-liquid chromatography (GLC). A series of common ionic liquids were evaluated as GLC stationary phases. It was found that many of these ionic liquids suffer from low thermal stability and possess unfavorable retention behavior for some classes of molecules. Two new ionic liquids were engineered and synthesized to overcome these drawbacks. The two new ionic liquids (1-benzyl-3-methylimidazolium trifluoromethanesulfonate and 1-(4-methoxyphenyl)-3-methylimidazolium trifluoromethanesulfonate) are based on "bulky" imidazolium cations with trifluoromethanesulfonate anions. Their solvation characteristics were evaluated using the Abraham solvation parameter model and correlations made between the structure of the cation and the degree to which the ionic liquids retain certain analytes. The new ionic liquids have good thermal stability up to 260 degrees C, provide symmetrical peak shapes, and because of their broad range of solvation-type interactions, exhibit dual-nature selectivity behavior. In addition, the ionic liquid stationary phases provided different retention behavior for many analytes compared to a commercial methylphenyl polysiloxane GLC stationary phase. This difference in selectivity is due to the unique solvation characteristics of the ionic liquids and makes them very useful as dualnature GLC stationary phases.
Probabilistic databases for decision analysis (1990)

A probabilistic database is defined in a previous article [R. Cavallo and M. Pittarelli, Proc. 13th Int. Conf. on Very Large Databases (VLDB); 1987; see Ref. 9] as a collection of probability distributions over Cartesian products of finite variable domains. the concept is extended here to accommodate interval-valued probabilities. Algebraic operations for both real- and interval-valued probabilities databases analogous to those for relational databases are defined. Techniques for making inferences regarding joint distributions on subsets of the variables over which a probabilistic database is defined are developed. These are illustrated through application to a problem of decision analysis under partial uncertainty. Connections between the probabilistic database formalism and other forms of data representation are discussed.
Novel classes of mouse repeated DNAs (1980)

Mouse DNA cleaved with Eco R11 (bst NI) displays two prominent restriction bands of 1.5 and 1.7 kb in agarose gels stained with ethidium bromide. These constitute novel subsets of repeated DNA in the mouse. Sequential Hoechst 33258-CsCl gradient fractionation of mouse DNA, yielding more GC rich main band DNA, and AT rich satellite DNA, revealed that both these fragments copurified with GC rich main band DNA. They were not detected in purified satellite preparations. Together these restriction bands constituted larger than or equal to 0.2% of main band DNAs. Hybridization of 32p labelled satellite DNA to blots of Eco R11 restricted mouse DNA showed positive hybridization only to smaller satellite restriction fragments, indicating satellite DNA had little or no homology with either the 1.5 or 1.7 kb fragments. The 1.5 and 1.7 kb fragments were isolated from gels and labelled with 32p by nick translation. Using a series of restriction endonucleases each of these two fragments showed different cleavage patterns. Filter hybridization confirmed that these two fragments were distinct subsets as they did not cross hybridize with each other. They also did not hybridize to other more minor repeated non-satellite DNA bands noted in ethidium bromide stained gels. Neither of them could be assigned to ribosomal genes as they did not hybridize to 32p kinase labelled 18S and 28S RNA. Isolation of DNA from male and female mice showed comparable amounts of both the 1.5 and 1.7 kb fragments. Thus neither was Y chromosome specific. From restriction patterns, and preliminary chromosome hybridization studies, these fragments are thought to represent "interspersed" repeated sequences rather than very long tandem (satellite like) centromeric arrays. The relationship between these repeated sequence subsets, their evolution and detailed organization, and their representation in different mouse species, remain to be determined.
Analytical Applications of Optical Imaging Fibers (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalytical Applications of Optical Imaging FibersPaul Pantano and David R. WaltCite this: Anal. Chem. 1995, 67, 15, 481A–487APublication Date (Print):August 1, 1995Publication History Published online4 June 2012Published inissue 1 August 1995https://pubs.acs.org/doi/10.1021/ac00111a727https://doi.org/10.1021/ac00111a727research-articleACS PublicationsRequest reuse permissionsArticle Views226Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Differential Surface Expression of ADAM10 and ADAM17 on Human T Lymphocytes and Tumor Cells (2013)

A disintegrin and metalloproteases (ADAMs) have been implicated in many processes controlling organismic development and integrity. Important substrates of ADAM proteases include growth factors, cytokines and their receptors and adhesion proteins. The inducible but irreversible cleavage of their substrates alters cell-cell communication and signaling. The crucial role of ADAM proteases (e.g. ADAM10 and 17) for mammalian development became evident from respective knockout mice, that displayed pre- or perinatal lethality with severe defects in many organs and tissues. Although many substrates for these two ADAM proteases were identified over the last decade, the regulation of their surface appearance, their enzymatic activity and their substrate specificity are still not well understood. We therefore analyzed the constitutive and inducible surface expression of ADAM10 and ADAM17 on a variety of human T cell and tumor cell lines. We demonstrate that ADAM10 is constitutively present at comparably high levels on the majority of the tested cell types. Stimulation with phorbol ester and calcium ionophore does not significantly alter the amount of surface ADAM10, except for a slight down-regulation from T cell blasts. Using FasL shedding as a readout for ADAM10 activity, we show that PKC activation and calcium mobilization are both prerequisite for activation of ADAM10 resulting in a production of soluble FasL. In contrast to ADAM10, the close relative ADAM17 is detected at only low levels on unstimulated cells. ADAM17 surface expression on T cell blasts is rapidly induced by stimulation. Since this inducible mobilization of ADAM17 is sensitive to inhibitors of actin filament formation, we propose that ADAM17 but not ADAM10 is prestored in a subcellular compartment that is transported to the cell surface in an activation- and actin-dependent manner.
ERA SPECIFIC BIOCHEMICAL RECURRENCE-FREE SURVIVAL FOLLOWING RADICAL PROSTATECTOMY FOR CLINICALLY LOCALIZED PROSTATE CANCER (2001)

Widespread early detection programs for prostate cancer resulted in downward stage migration in men presenting with prostate cancer at our institution during the last 18 years. Also, we have demonstrated a biochemical recurrence-free survival advantage, probably secondary to an improved therapeutic outcome as well as lead time bias, in men who underwent surgery between 1989 and 1998, compared with those between 1982 and 1988. When trying to compare the efficacy of different treatment modalities for prostate cancer, the era in which patients underwent therapy is an important factor to be considered.
Color Filter Array and Color Correction for High Dynamic Range CMOS Image Sensors (2001)

This contribution presents a novel approach to CMOS color image sensors featuring high dynamic range. First we introduce a novel Color-Filter-Array (CFA) that has been especially developed for CMOS imager featuring high dynamic range. Then color correction method is presented that is made with a fast algorithm realized using a Look-Up-Table (LUT). The LUT is created off-chip to improve a high quality and keep the on-chip logic small.
Synthesis of Truncated Analogues for Studying the Process of Glycosyl Phosphatidylinositol Modification (2010)

Many eukaryotic proteins are modified with a glycosylphosphatidylinositol (GPI) anchor at their C-termini. This post-translational modification causes these proteins to be noncovalently tethered to the plasma membrane. The synthesis of truncated GPI anchor analogues is reported; these compounds were designed for use as soluble substrates for GPI transamidase (GPI-T), the enzyme that appends the GPI anchor onto proteins.
Identificación, evaluación y control de riesgos en las fábricas de piensos compuestos (1996)

First and second molar extraction, in combination with orthodontic treatment, is well represented in the literature. The following cases document situations in which first or second molars were removed without the introduction of orthodontic forces. The results show space gain and good eruption of the replacing second and third molars.
Fabrication of Superhydrophobic PDMS Surfaces by Combining Acidic Treatment and Perfluorinated Monolayers (2009)

In this paper, polydimethylsiloxane (PDMS) with a superhydrophobic surface was generated by the combination of an acid corrosion followed by the covalent grafting of a highly fluorinated monolayer. The acid corrosion was performed with H2SO4 or HF, and the more effective was concentrated H2SO4. The resulting surface had a contact angle with water of 135 degrees. All the acid-treated samples were then functionalized by the covalent grafting of triethoxyaminopropylsilane followed by the reaction with semifluorinated acid chlorides, via the formation of an amide bond, or directly by a commercially available highly fluorinated silane, 1H,1H,2H,2H-perfluorodecyltriethoxysilane, to afford superhydrophobic surfaces (contact angle with water exceeding 160 degrees). The introduction of an amide function in the fluorinated monolayer afforded the best water repellency properties probably due to the organization induced by H-bonding between the surface grafted molecules.
Site-specific mutagenesis of the human fibroblast interferon gene. (1984)

Human fibroblast interferon has three cysteine residues, located at amino acid positions 17, 31, and 141. Using the technique of site-specific mutagenesis with a synthetic oligonucleotide primer, we changed the codon for cysteine-17 to a codon for serine. The resulting interferon, IFN-beta Ser-17, retains the antiviral, natural killer cell activation, and antiproliferative activities of native fibroblast interferon. The purified IFN-beta Ser-17 protein has an antiviral specific activity of 2 X 10(8) units/mg, similar to that of purified native fibroblast interferon. In addition, the purified protein is stable to long-term storage at -70 degrees C.
Diazo Transfer and Click Chemistry in the Solid Phase Syntheses of Lysine-Based Glycodendrimers as Antagonists against Escherichia coli FimH (2011)

Uropathogenic Escherichia coli infections, ultimately leading to cystitis and pyelonephritis, are initially mediated by the adhesion of the bacterial FimH to the transmembrane glycoprotein uroplakin-1a present at the surface of urothelial cells. The adhesion is based on the recognition and high avidity binding between the high-mannose glycans of the uroplakin and the FimH, a mannose-specific lectin located at the tip of type 1 fimbriae. We found that synthetic multiantennary mannopyranosides glycodendrons, harboring triazole functionality at the anomeric position, were potent hemagglutination inhibitors of guinea pig erythrocytes and E. coli. A mannosylated dendrimer exposing up to sixteen sugar residues showed an HAI titer of 1 μM and was thus 500-fold more potent than the corresponding monovalent methyl α-d-mannopyranoside. The synthesis of the glycodendrons involved highly efficient solid-phase synthesis of branched l-lysine scaffolds, diazo transfer reaction on the terminal amine residues, and 1,3-dipolar copper-catalyzed azide-alkyne cycloaddition using propargyl α-d-mannopyranoside.
Modeling and Control of Fuel Cell Based Distributed Generation Systems in a Standalone AC Power Supply (2005)

This paper develops a circuit model and con- trollers of fuel cell based distributed generation systems (DGS) in a standalone AC power supply. Dynamic model of the fuel cell is considered. To boost low out- put DC voltage of the fuel cell to high DC voltage and compensate for its slow response during the transient, two full-bridge DC to DC converters are adopted and their controllers are designed: a unidirectional full- bridge DC to DC boost converter for the fuel cell and a bidirectional full-bridge DC to DC buck/boost convert- er for the battery. For a three-phase DC to AC inverter, a discrete-time state space model in the stationary dq reference frame is derived and two discrete-time sliding mode controllers are designed: voltage controller in the outer loop and current controller in the inner loop. To demonstrate the proposed circuit model and control strategies, a simulation test-bed using Matlab/Simulink is developed and various results are given.
Cationic liposomes induce apoptosis through p38 MAP kinase–caspase-8–Bid pathway in macrophage-like RAW264.7 cells (2005)

We have demonstrated that cationic liposomes composed of stearylamine (SA-liposomes) induce apoptosis in a variety of cells, but the mechanism responsible for the cellular death is not clear. In this paper, we investigated the signaling pathways implicated in SA-liposome-induced apoptosis in the macrophage-like cell line RAW264.7. Treatment with SA-liposomes caused the activation of mitogen-activated protein kinases (MAPKs), especially p38 and c-jun N-terminal kinase, and apoptosis was only inhibited upon the addition of a specific inhibitor for p38. N-acetylcysteine, a scavenger of reactive oxygen species (ROS), effectively inhibited the activation of p38 and cellular death, indicating that the activation induced by ROS is an initial step in the process of apoptosis triggered by SA-liposomes. Caspase-8 was activated by p38, and caspase-8-dependent cleavage of Bid was also observed. No down-regulation of bcl-2 expression, and no cleavage of Bax protein were observed. Taken together, our results suggest that apoptosis of RAW264.7 by SA-liposomes was mediated by the MAPK p38 and a caspase-8-dependent Bid-cleavage pathway. Moreover, we found that ROS can contribute intimately to the SA-liposome-induced cell death in RAW264.7.
Effects of mutation of the CREB binding site of the somatostatin promoter on cyclic AMP responsiveness in CV-1 cells. (1993)

The transcription factors CREB (cAMP response element binding protein) and ATF (activating transcription factor) recognize DNA containing the consensus sequence TGACGTCA. We compared the neuropeptide somatostatin promoter, which binds CREB and is activated by cAMP, to the adenovirus E2A promoter, which binds ATF but is not activated by cAMP, to determine which specific nucleotides within a CREB/ATF recognition sequence confer cAMP responsiveness. Several mutant somatostatin promoters were generated containing part of all of the E2A ATF binding site. Some of the hybrid CREB/ATF binding sites competed for factor binding to a wild-type somatostatin promoter probe. However, only the wild-type CREB binding site promoter could confer cAMP activation on a linked CAT plasmid. Furthermore, this wild-type CREB binding site could confer cAMP activation on the CAT plasmid only if it was adjacent to a wild-type somatostatin TATA box and cap site. These results suggest that slight deviation from a wild-type CREB recognition sequence might be tolerated by factor(s) binding to cAMP response element-like sequences. However, transcription activation may require a particular CREB recognition sequence, as well as additional promoter elements that bind proteins that interact with CREB.
Analyzing visual layout for a non-visual presentation-document interface (2006)

Presentation documents play important roles in many fields, such as business and education. The principal purpose of presentation documents is to convey information visually, so recognizing the visual layout is essential for understanding those documents. However it is inherently difficult for the blind people to recognize a visual layout, because there are numerous types of charts in presentation documents. As the first step to solve such problems, this study focuses on diagrams in which objects or groups of objects are bound by arrows. Such diagrams usually show relationships among the objects. If such relationships could be recognized by screen readers, it would make them accessible. However, the presentation authoring applications do not have functions for embedding these relationships among objects. Therefore this paper proposes a visual analysis method for diagram structure in presentation documents to automatically create metadata. It generates metadata which describes the relationships of objects, and the source-destination relationships of arrows. Then a novel interface utilizing the metadata was prototyped to present the visual structure of presentation documents in a tree view. This allows blind users to understand presentation documents easily, because it represents the visual structure that current screen readers cannot expose. In addition, they are familiar with the tree view interface, so they can use it without training. Finally, an evaluation shows that our method for automatically creating the metadata can be applied to various types of diagrams in presentation documents.
Stem Cells to Pancreatic β-Cells: New Sources for Diabetes Cell Therapy (2009)

The number of patients worldwide suffering from the chronic disease diabetes mellitus is growing at an alarming rate. Insulin-secreting beta-cells in the islet of Langerhans are damaged to different extents in diabetic patients, either through an autoimmune reaction present in type 1 diabetic patients or through inherent changes within beta-cells that affect their function in patients suffering from type 2 diabetes. Cell replacement strategies via islet transplantation offer potential therapeutic options for diabetic patients. However, the discrepancy between the limited number of donor islets and the high number of patients who could benefit from such a treatment reflects the dire need for renewable sources of high-quality beta-cells. Human embryonic stem cells (hESCs) are capable of self-renewal and can differentiate into components of all three germ layers, including all pancreatic lineages. The ability to differentiate hESCs into beta-cells highlights a promising strategy to meet the shortage of beta-cells. Here, we review the different approaches that have been used to direct differentiation of hESCs into pancreatic and beta-cells. We will focus on recent progress in the understanding of signaling pathways and transcription factors during embryonic pancreas development and how this knowledge has helped to improve the methodology for high-efficiency beta-cell differentiation in vitro.
Characterization and Repeat Analysis of the Compact Genome of the Freshwater Pufferfish Tetraodon nigroviridis (2000)

Tetraodon nigroviridis is a freshwater pufferfish 20–30 million years distant from Fugu rubripes . The genome of both tetraodontiforms is compact, mostly because intergenic and intronic sequences are reduced in size compared to other vertebrate genomes. The previously uncharacterized Tetraodon genome is described here together with a detailed analysis of its repeat content and organization. We report the sequencing of 46 megabases of bacterial artificial chromosome (BAC) end sequences, which represents a random DNA sample equivalent to 13% of the genome. The sequence and location of rRNA gene clusters, centromeric and subtelocentric satellite sequences have been determined. Minisatellites and microsatellites have been cataloged and notable differences were observed in comparison with microsatellites from Fugu . The genome contains homologies to all known families of transposable elements, including Ty3-gypsy, Ty1-copia, Line retrotransposons, DNA transposons, and retroviruses, although their overall abundance is &lt;1%. This structural analysis is an important prerequisite to sequencing the Tetraodon genome. [The sequence data described in this paper have been submitted to the EMBL data library under accession nos. AJ245809 , AJ270048 , AJ245808 , AJ270029 – AJ270047 , DS42722 and AL305790 – AL352938 .]
Multi-document summarization using sentence-based topic models (2009)

Most of the existing multi-document summarization methods decompose the documents into sentences and work directly in the sentence space using a term-sentence matrix. However, the knowledge on the document side, i.e. the topics embedded in the documents, can help the context understanding and guide the sentence selection in the summarization procedure. In this paper, we propose a new Bayesian sentence-based topic model for summarization by making use of both the term-document and term-sentence associations. An efficient variational Bayesian algorithm is derived for model parameter estimation. Experimental results on benchmark data sets show the effectiveness of the proposed model for the multi-document summarization task.
Inhibition of Mac-1 (CD11b/CD18) enhances tumor response to radiation by reducing myeloid cell recruitment (2010)

Despite recent advances in radiotherapy, loco-regional failures are still the leading cause of death in many cancer patients. We have previously reported that bone marrow-derived CD11b + myeloid cells are recruited to tumors grown in irradiated tissues, thereby restoring the vasculature and tumor growth. In this study, we examined whether neutralizing CD11b monoclonal antibodies could inhibit the recruitment of myeloid cells into irradiated tumors and inhibit their regrowth. We observed a significant enhancement of antitumor response to radiation in squamous cell carcinoma xenografts in mice when CD11b antibodies are administered systemically. Histological examination of tumors revealed that CD11b antibodies reduced infiltration of myeloid cells expressing S100A8 and matrix metalloproteinase-9. CD11b antibodies further inhibited bone marrow-derived cell adhesion and transmigration to C166 endothelial cell monolayers and chemotactic stimuli, respectively, to levels comparable to those from CD11b knockout or CD18 hypomorphic mice. Given the clinical availability of humanized CD18 antibodies, we tested two murine tumor models in CD18 hypomorphic or CD11b knockout mice and found that tumors were more sensitive to irradiation when grown in CD18 hypomorphic mice but not in CD11b knockout mice. When CD18 hypomorphism was partially rescued by reconstitution with the wild-type bone marrow, the resistance of the tumors to irradiation was restored. Our study thus supports the rationale of using clinically available Mac-1 (CD11b/CD18) antibodies as an adjuvant therapy to radiotherapy.