gebhart/patrepeval huggingface dataset:
Clinical characteristics and causes of pruritus in cats: a multicentre study on feline hypersensitivity‐associated dermatoses (2011)

Hypersensitivity dermatitides (HD) are often suspected in cats. Cats with HD are reported to present with one or more of the following patterns: miliary dermatitis, eosinophilic dermatitis, self-induced symmetrical alopecia or head and/or neck excoriations. Previous reports on feline HD included small numbers of animals, took place in geographically restricted areas or did not compare these conditions with other causes of pruritus. The goal of the present study was to analyse 72 parameters covering signalment, clinical, laboratory and treatment characteristics from a large group of pruritic cats from different geographical areas. Of the 502 cats, the following diagnoses were made: flea HD (29% of cases), food HD (12%) nonflea/nonfood HD (20%) and other diseases in which pruritus was a feature (24%). Cats with signs consistent with a HD but which did not complete a food trial were not analysed further (15% of cases). Most cats with nonflea HD exhibited signs compatible with one or more of the four typical lesional patterns, but none of these patterns was found to be pathognomonic for any specific diagnosis. Food HD and nonflea/nonfood HD were found to be clinically undistinguishable. Young adult, purebred and female cats appeared predisposed to nonflea/nonfood HD. As many diagnoses presented with similar lesional patterns, a thorough clinical work-up is required for establishment of a specific diagnosis.
Mimecan, the 25-kDa Corneal Keratan Sulfate Proteoglycan, Is a Product of the Gene Producing Osteoglycin (1997)

Bovine cornea contains three unique keratan sulfate proteoglycans (KSPGs), of which two (lumican and keratocan) have been characterized using molecular cloning. The gene for the third protein (KSPG25) has not been identified. This study examined the relationship between the KSPG25 protein and the gene for osteoglycin, a 12-kDa bone glycoprotein. The N-terminal amino acid sequence of KSPG25 occurs in osteoglycin cDNA cloned from bovine cornea. The osteoglycin amino acid sequence makes up the C-terminal 47% of the deduced sequence of the KSPG25 protein. Antibodies to osteoglycin reacted with intact corneal KSPG, with KSPG25 protein, and with a 36-kDa protein, distinct from lumican and keratocan. KSPG25-related proteins, not modified with keratan sulfate, were also detected in several connective tissues. Northern blot analysis showed mRNA transcripts of 2.4, 2.5, and 2.6 kilobases in numerous tissues with the 2.4-kilobase transcript enriched in ocular tissues. Ribonuclease protection analysis detected several protected KSPG25 mRNA fragments, suggesting alternate splicing of KSPG25 transcripts. We conclude that the full-length translation product of the gene producing osteoglycin is a corneal keratan sulfate proteoglycan, also present in many non-corneal tissues without keratan sulfate chains. The multiple size protein products of this gene appear to result from in situ proteolytic processing and/or alternative splicing of mRNA. The name mimecan is proposed for this gene and its products.
Drug Delivery to Macrophages for the Therapy of Cancer and Infectious Diseases (1987)

The mechanisms by which mononuclear phagocytes discriminate between self and nonself, recognize foreign materials, senescent, damaged, old, or effete cells, and tumor cells are unknown. However, regardless of the mechanism(s) involved, once activated by the appropriate signal(s), macrophages are able to selectively recognize and destroy neoplastic cells in vitro and in vivo. Liposomes injected intravenously, in common with other particulate or polymeric matrices, localize preferentially in organs with high mononuclear phagocyte activity and in circulating blood monocytes. This behavior allows microparticulates to serve as a convenient system for the selective delivery of encapsulated drugs to cells of the mononuclear phagocyte series in vivo. Liposomes are a particularly attractive experimental system because of their capacity to incorporate a wide variety of water-soluble and lipid-soluble drugs. At this time, however, there is no reason to assume that a liposome-based drug delivery system will offer any significant therapeutic advantage compared to other microparticulate drug delivery systems. As in commercial development of any pharmaceutical preparation, considerations of cost-of-goods, shelf life, and acceptance of the formulation and dosing regimen by both physicians and patients will be of major importance in determining success and widespread clinical use. Liposomes containing macrophage-activating agents are highly effective at augmenting macrophage-mediated tumoricidal activity in vitro eradicating tumor metastasis in vivo, as well as protecting animals from a wide variety of microbial and viral infections. Although the demands of solving the scientific and technical problems associated with liposome development are substantial, the rapid rate of progress in biology and in pharmaceutical sciences enhances the prospect of success for at least several aspects of liposome-mediated drug delivery. The next few years will be crucial in determining whether the commercial development of liposomes is feasible or whether they will join the ranks of other drug carrier designs that have failed to fulfill their initial promise.
Introduction (1993)

The chaplain who reviewed Volume 1 of Death with Dignity remarked: ‘Given that many of the staff of our hospitals also reflect the multicultural society, there should, ideally, be a section on Christianity, since many staff may not be familiar with the Christian tradition’1.
Computational identification of the Spo0A-phosphate regulon that is essential for the cellular differentiation and development in Gram-positive spore-forming bacteria (2003)

Spo0A-phosphate is essential for the initiation of cellular differentiation and developmental processes in Gram-positive spore-forming bacteria. Here we combined comparative genomics with analyses of microarray expression profiles to identify the Spo0A-phosphate regulon in Bacillus subtilis. The consensus Spo0A-phosphate DNA-binding motif identified from the training set based on different computational algorithms is an 8 bp sequence, TTGTCGAA. The same motif was identified by aligning the upstream regulatory sequences of spo0A-dependent genes obtained from the expression profile of Sad67 (a constitutively active form of Spo0A) and their orthologs. After the transcription units (TUs) having putative Spo0A-phosphate binding sites were obtained, conservation of regulons among the genomes of B.subtilis, Bacillus halodurans and Bacillus anthracis, and expression profiles were employed to identify the most confident predictions. Besides genes already known to be directly under the control of Spo0A-phosphate, 276 novel members (organized in 109 TUs) of the Spo0A-phosphate regulon in B.subtilis are predicted in this study. The sensitivity and specificity of our predictions are estimated based on known sites and combinations of different types of evidence. Further characterization of the novel candidates will provide information towards understanding the role of Spo0A-phosphate in the sporulation process, as well as the entire genetic network governing cellular differentiation and developmental processes in B.subtilis.
Refractive Index of Water and Its Dependence on Wavelength, Temperature, and Density (1985)

A survey of the available experimental data and the existing equations for the refractive index of water is given. The dependence of the molar refraction on wavelength, temperature, and density is shown over an extended range. Based upon the electromagnetic theory of light an equation for the refractive index of water with wavelength, temperature, and density as independent variables is constructed. Its coefficients are directly deduced from all available experimental data by least-squares fit. The range of validity of wavelength is restricted by the theory for normal dispersion to 182 nm≤λ≤2770 nm. The range of temperature and density is given by the available experimental data. Interpolations between the single measured points are possible and the following range of validity can be recommended: for temperature −10 °C≤T≤500 °C and for density 0.0028 kg/m3 ≤ρ≤1045 kg/m3. Good agreement exists between the new relation, the available experimental data, and several existing equations.
Construction of Polyfunctional Coatings Assisted by Gallic Acid to Facilitate Co-Immobilization of Diverse Biomolecules (2013)

Designing a multifunctional surface based on the coimmobilization of two or more diverse biomolecules with synergic action is very important in certain cases. In this work, a facile method by two-step aimed to construct a polyfunctional coating containing -COOH, -NH2, and phenol/quinine groups was reported. The first-step was to introduce amine groups onto target modified-surface by coating with plasma polymerized allylamine (PPAam), followed by the second-step conjugation of gallic acid (3,4,5-trihydroxybenzoic acid) onto the PPAam surface. The density of -COOH, -NH2, and phenol/quinone groups could be regulated easily by adjusting the reaction time of GA conjugation, making it possible to coimmobilize two or three diverse molecules. This has been shown by the successful coimmobilization of anti-CD34 antibody and vascular endothelial growth factor (VEGF). The surface coimmobilized with the anti-CD34 antibody and VEGF presented significant enhancement in the capture of endothelial progenitor cells (EPCs) and the growth of human umbilical vein endothelial cells (HUVECs). These data suggest the huge potential of such polyfunctional coating for tailoring the desired interfacial properties of materials through selectively conjugating two or more diverse bioactive molecules with synergic action.
Self-learning based Fourier ptychographic microscopy (2015)

Fourier Ptychographic Microscopy (FPM) is a newly proposed computational imaging method aimed at reconstructing a high-resolution wide-field image from a sequence of low-resolution images. These low-resolution images are captured under varied illumination angles and the FPM recovery routine then stitches them together in the Fourier domain iteratively. Although FPM has achieved success with static sample reconstructions, the long acquisition time inhibits real-time application. To address this problem, we propose here a self-learning based FPM which accelerates the acquisition and reconstruction procedure. We first capture a single image under normally incident illumination, and then use it to simulate the corresponding low-resolution images under other illumination angles. The simulation is based on the relationship between the illumination angles and the shift of the sample's spectrum. We analyze the importance of the simulated low-resolution images in order to devise a selection scheme which only collects the ones with higher importance. The measurements are then captured with the selection scheme and employed to perform the FPM reconstruction. Since only measurements of high importance are captured, the time requirements of data collection as well as image reconstruction can be greatly reduced. We validate the effectiveness of the proposed method with simulation and experimental results showing that the reduction ratio of data size requirements can reach over 70%, without sacrificing image reconstruction quality.
Toward oligosaccharide- and glycopeptide-based HIV vaccines. (2006)

The human immunodeficiency virus (HIV) is the causative agent of acquired immune deficiency syndrome (AIDS), and HIV type 1 (HIV-1) is responsible for the current global pandemic of HIV and AIDS. There is widespread agreement that the best hope to contain this pandemic is the development of an effective HIV-1 vaccine. The major difficulty in HIV vaccine design, however, is in identifying novel epitopes for inducing broadly neutralizing antibodies. Accumulating data have implied that the HIV-1 carbohydrates, which are a strong defense against host immune attack, can also serve as targets for vaccines. This notion was reinforced by the characterization of a novel oligomannose cluster on HIV-1 glycoprotein gp120 as the epitope of the broadly neutralizing antibody 2G12. This review describes the current status and prospective of the progress toward a carbohydrate-based HIV vaccine. Recent research on the design, synthesis and antigenicity of HIV-1 oligosaccharides and glycopeptide immunogens is also discussed.
l-Phenylalanine Production by Analog-resistant Mutants ofCorynebacterium glutamicum (1974)

Journal Article l-Phenylalanine Production by Analog-resistant Mutants of Corynebacterium glutamicum Get access Hiroshi Hagino, Hiroshi Hagino Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo Search for other works by this author on: Oxford Academic Google Scholar Kiyoshi Nakayama Kiyoshi Nakayama Tokyo Research Laboratory, Kyowa Hakko Kogyo Co., Ltd., Machida-shi, Tokyo Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 38, Issue 1, 1 January 1974, Pages 157–161, https://doi.org/10.1080/00021369.1974.10861130 Published: 01 January 1974 Article history Received: 17 August 1973 Published: 01 January 1974
A compact neutron generator using a field ionization source (2012)

Field ionization as a means to create ions for compact and rugged neutron sources is pursued. Arrays of carbon nano-fibers promise the high field-enhancement factors required for efficient field ionization. We report on the fabrication of arrays of field emitters with a density up to 10(6) tips∕cm(2) and measure their performance characteristics using electron field emission. The critical issue of uniformity is discussed, as are efforts towards coating the nano-fibers to enhance their lifetime and surface properties.
Organic Electroluminescent Device with a Three-Layer Structure (1988)

An electroluminescent device with a three-layer structure was constructed using an intensely fluorescent material, 12-phthaloperinone derivative, for the emitter material. The cell structure of Mg-electrode/amorphous electron-transport layer/polycrystalline emitting layer/amorphous hole-transport layer/Au-electrode was constructed on a glass substrate. Yellow EL emission was clearly observed in normal room lighting at the dc bias voltage of 60 V. The emission intensity was proportional to the injection current over a wide current range of 10 -7 to 10 -3 A/cm 2 . The EL intensity exceeded 1 µW/cm 2 at the injection current of 2 mA/cm 2 .
PatchTable (2015)

This paper presents a data structure that reduces approximate nearest neighbor query times for image patches in large datasets. Previous work in texture synthesis has demonstrated real-time synthesis from small exemplar textures. However, high performance has proved elusive for modern patch-based optimization techniques which frequently use many exemplar images in the tens of megapixels or above. Our new algorithm, PatchTable, offloads as much of the computation as possible to a pre-computation stage that takes modest time, so patch queries can be as efficient as possible. There are three key insights behind our algorithm: (1) a lookup table similar to locality sensitive hashing can be precomputed, and used to seed sufficiently good initial patch correspondences during querying, (2) missing entries in the table can be filled during pre-computation with our fast Voronoi transform, and (3) the initially seeded correspondences can be improved with a precomputed k-nearest neighbors mapping. We show experimentally that this accelerates the patch query operation by up to 9× over k-coherence, up to 12× over TreeCANN, and up to 200× over PatchMatch. Our fast algorithm allows us to explore efficient and practical imaging and computational photography applications. We show results for artistic video stylization, light field super-resolution, and multi-image editing.
Clinical use of Prostate Specific Antigen in Patients with Prostate Cancer (1989)

The clinical use of prostate specific antigen as a screening test for prostate cancer, as a preoperative determinant for staging of prostate cancer and to monitor response to therapy in prostatic cancer patients was evaluated in 168 men with benign prostatic hyperplasia and 231 men with prostate cancer. Only 3% of the men with benign prostatic hyperplasia had prostate specific antigen levels greater than 10 ng. per ml. compared to 44% of the men with proved prostate cancer. Preoperative prostate specific antigen levels increased with higher clinical stages of prostate cancer but there was substantial overlap among stages. Among patients with stage A1 prostate cancer who were followed expectantly none had an elevated prostate specific antigen value or metastatic disease during a followup of 15 to 120 months. After radical prostatectomy serum prostate specific antigen values decreased to undetectable levels (less than 0.6 ng. per ml.) in 89% of the patients with organ-confined disease, in 87% of those with microscopically positive margins only but in only 34% with seminal vesicles or lymph node involvement. Failure of the prostate specific antigen levels to decrease to the undetectable range after radical prostatectomy was associated with a greater likelihood of subsequent tumor recurrence. Only 3 of 18 patients (17%) treated with definitive radiation therapy had post-irradiation prostate specific antigen values of less than 0.6 ng. per ml., while in 39% the prostate specific antigens values remained greater than 4 ng. per ml. and in 4 of 18 (22%) the values were greater than 10 ng. per ml. Of patients with previously untreated stage D2 prostate cancer the mean pre-treatment prostate specific antigen value was 63.7 ng. per ml. compared to a post-hormonal therapy mean value of 31.1 ng. per ml. Of 32 patients treated with hormonal therapy 14 had stable disease, including 13 with prostate specific antigen levels of less than 10 ng. per ml. In contrast, 18 patients had progressive disease, of whom 16 had prostate specific antigen levels of more than 10 ng. per ml. We conclude that the serum prostate specific antigen assay is most useful clinically to monitor the response to therapy of prostate cancer patients.
Adaptation of the endothelium to fluid flow: in vitro analyses of gene expression and in vivo implications (2004)

Biomechanical forces generated by blood flow play an important role in the pathogenesis of vascular disease. For example, regions exposed to non-uniform shear stresses develop early atherosclerotic lesions while areas exposed to uniform shear stresses are protected. A variety of in vitro flow apparatuses have been created to apply well-characterized flow patterns to endothelial cells in an effort to dissect the cellular and molecular pathways involved in these distinct processes. Recent advances in biotechnology have permitted large-scale transcriptional profiling techniques to replace candidate gene screens and have allowed the genome-wide examination of biomechanical force-induced endothelial gene expression profiles. This review provides an overview of biomechanical force-induced modulation of endothelial phenotype. It examines the effect of sustained laminar shear stress (LSS), a type of uniform shear stress, on in vitro endothelial gene expression by synthesizing data from the early candidate gene and differential display polymerase chain reaction (PCR) approaches to the numerous, recent, high throughput functional genomic analyses. These studies demonstrate that prolonged LSS regulates the expression of only a small percentage (1-5%) of endothelial genes, and this transcriptional profile produces an endothelial phenotype that is quiescent, being protected from apoptosis, inflammation and oxidative stress. These observations provide a possible molecular mechanism for the strong correlation between patterns of blood flow and the occurrence of vascular pathologies, such as atherosclerosis, in vivo.
The Vaccinia Virus 14-Kilodalton (A27L) Fusion Protein Forms a Triple Coiled-Coil Structure and Interacts with the 21-Kilodalton (A17L) Virus Membrane Protein through a C-Terminal α-Helix (1998)

The vaccinia virus 14-kDa protein (encoded by the A27L gene) plays an important role in the biology of the virus, acting in virus-to-cell and cell-to-cell fusions. The protein is located on the surface of the intracellular mature virus form and is essential for both the release of extracellular enveloped virus from the cells and virus spread. Sequence analysis predicts the existence of four regions in this protein: a structureless region from amino acids 1 to 28, a helical region from residues 29 to 37, a triple coiled-coil helical region from residues 44 to 72, and a Leu zipper motif at the C terminus. Circular dichroism spectroscopy, analytical ultracentrifugation, and chemical cross-linking studies of the purified wild-type protein and several mutant forms, lacking one or more of the above regions or with point mutations, support the above-described structural division of the 14-kDa protein. The two contiguous cysteine residues at positions 71 and 72 are not responsible for the formation of 14-kDa protein trimers. The location of hydrophobic residues at the a and d positions on a helical wheel and of charged amino acids in adjacent positions, e and g, suggests that the hydrophobic and ionic interactions in the triple coiled-coil helical region are involved in oligomer formation. This conjecture was supported by the construction of a three-helix bundle model and molecular dynamics. Binding assays with purified proteins expressed in Escherichia coli and cytoplasmic extracts from cells infected with a virus that does not produce the 14-kDa protein during infection (VVindA27L) show that the 21-kDa protein (encoded by the A17L gene) is the specific viral binding partner and identify the putative Leu zipper, the predicted third alpha-helix on the C terminus of the 14-kDa protein, as the region involved in protein binding. These findings were confirmed in vivo, following transfection of animal cells with plasmid vectors expressing mutant forms of the 14-kDa protein and infected with VVindA27L. We find the structural organization of 14kDa to be similar to that of other fusion proteins, such as hemagglutinin of influenza virus and gp41 of human immunodeficiency virus, except for the presence of a protein-anchoring domain instead of a transmembrane domain. Based on our observations, we have established a structural model of the 14-kDa protein.
Chelate Restrained Boron Cations for Intermolecular Electrophilic Arene Borylation (2009)

Highly electrophilic boron species that borylate arenes are generated by halide abstraction from CatBX (Cat = catecholato, C6H4O22−, X = Cl or Br) by [Et3Si][CbBr6] (CbBr6 = [closo-1-H-CB11H5Br6]−). A transient [CatB][CbBr6] related species reacts as a synthetic equivalent of [CatB]+ in intermolecular electrophilic borylation, with reactions proceeding rapidly at 25 °C. The [CatB]+ moiety was shown to be strongly Lewis acidic on the basis of 1H and 31P{1H} NMR spectroscopy of the crotonaldehyde and triethylphosphine oxide adducts, respectively. Catalytic quantities of [Et3Si][CbBr6] and CatBX were effective for the high-yielding borylation of arenes by CatBH in a highly atom efficient cycle with H2 the only byproduct. Successful catalysis was dependent on the robust [CbBr6]− anion and the use of electrophile-resistant borane sources.