gebhart/patrepeval huggingface dataset:
A prefilled, ready-to-use, electrophoresis-based lab-on-a-chip device for monitoring ions in blood and urine (2010)

We present the Medimate Multireader®, the first point-of-care Lab-on-a-Chip device based on Capillary Electropho-resis. We show that it is possible to analyze lithium in whole blood and sodium in urine, using a commercially viable Multireader that is suitable for self testing. In addition, we demonstrate the ability to detect creatinine using the same platform.
Luminal membrane expression of mesothelin is a prominent poor prognostic factor for gastric cancer (2012)

We described that the luminal membrane expression of mesothelin was a reliable prognostic factor in gastric cancer, suggesting the functional significance of membrane-localised mesothelin in the aggressive behaviour of gastric cancer cells.
High-resolution capacitance measurement and potentiometry by force microscopy (1988)

We demonstrate the usefulness and high sensitivity of the atomic force microscope (AFM) for imaging surface dielectric properties and for potentiometry through the detection of electrostatic forces. Electric forces as small as 10−10 N have been measured, corresponding to a capacitance of 10−19 farad. The sensitivity of our AFM should ultimately allow us to detect capacitances as low as 8×10−22 F. The method enables us to detect the presence of dielectric material over Si, and to measure the voltage in a p-n junction with submicron spatial resolution.
Analysis of HIV cross-resistance to protease inhibitors using a rapid single-cycle recombinant virus assay for patients failing on combination therapies (1999)

These results suggest that, although the total number of protease mutations correlates with the degree of cross-resistance, the specific mechanisms accounting for primary resistance and for cross-resistance may be different.
Differential leukocyte counting via fluorescent detection and image processing on a centrifugal microfluidic platform (2016)

Centrifugal microfluidics has received much attention in the last decade for the automation of blood testing at the point-of-care, specifically for the detection of chemistries, proteins, and nucleic acids. However, the detection of common blood cells on-disc, particularly leukocytes, remains a challenge. In this paper, we present two analytical methods for enumerating leukocytes on a centrifugal platform using a custom-built fluorescent microscope, acridine orange nuclear staining, and image processing techniques. In the first method, cell analysis is performed in glass capillary tubes; in the second, acrylic chips are used. A bulk-cell analysis approach is implemented in both cases where the pixel areas of fractionated lymphocyte/monocyte and granulocyte layers are correlated with cell counts. Generating standard curves using porcine blood sample controls, we observed strong linear fits to measured cell counts using both methods. Analyzing the pixel intensities of the fluorescing white cell region, we are able to differentiate lymphocytes from monocytes via pixel clustering, demonstrating the capacity to perform a 3-part differential. Finally, a discussion of pros and cons of the bulk-cell analysis approach concludes the paper.
Insensitivity of a synthetic peptide-based test (Pepti-LAV 1–2) for the diagnosis of HIV infection in African children (1995)

Brattegaard, K.; Soroh, D.; Zadi, F.; Digbeu, H.; Vetter, K. M.; De Cock, K. M. Author Information
Mutant human cells defective in induction of major histocompatibility complex class II genes by interferon gamma. (1993)

Using immunoselection, we have isolated 11 independent mutant HT1080 fibrosarcoma cell lines defective in the induction by interferon gamma (IFN-gamma) of the expression of the human leukocyte antigen HLA-DRA. The mutations are recessive and fall into five complementation groups. All the mutants are affected mainly in the expression of major histocompatibility complex class II and invariant-chain genes. Type I mutants (three complementation groups) are completely defective in induction of the invariant-chain and class II HLA-DP, -DQ, -DR, and -DM genes, whereas type II mutants (two complementation groups) induce these genes weakly in response to IFN-gamma, in the order DPB > DRA > invariant chain. The induction by IFN-gamma of the mRNAs for class I, TAP1, LMP7, and 9-27 is partially defective and the induction of the proteins IRF-1 and ICAM-1 is normal in both types of mutants. All the mutants respond normally to IFN-alpha. The mutants are stable and thus can be used to clone the affected genes by reversion.
Solid-state low-loss intracavity saturable absorber for Nd:YLF lasers: an antiresonant semiconductor Fabry–Perot saturable absorber (1992)

We introduce a new low-loss fast intracavity semiconductor Fabry-Perot saturable absorber operated at anti-resonance both to start and sustain stable mode locking of a cw-pumped Nd:YLF laser. We achieved a 3.3-ps pulse duration at a 220-MHz repetition rate. The average output power was 700 mW with 2 W of cw pump power from a Ti:sapphire laser. At pump powers of less than 1.6 W the laser self-Q switches and produces 4-ps pulses within a 1.4-micros Q-switched pulse at an approximately 150-kHz repetition rate determined by the relaxation oscillation of the Nd:YLF laser. Both modes of operation are stable. In terms of coupled-cavity mode locking, the intra-cavity antiresonant Fabry-Perot saturable absorber corresponds to monolithic resonant passive mode locking.
Mercaptopurine pharmacogenetics: monogenic inheritance of erythrocyte thiopurine methyltransferase activity. (1980)

Thiopurine methyltransferase (TPMT) catalyzes thiopurine S-methylation, an important metabolic pathway for drugs such as 6-mercaptopurine. Erythrocyte (RBC) TPMT activity was measured in blood samples from 298 randomly selected subjects. Of the subjects, 88.6% were included in a subgroup with high enzyme activity (13.50 +/- 1.86 U, mean +/- SD), 11.1% were included in a subgroup with intermediate activity (7.20 +/- 1.08 U), and 0.3% had undetectable activity. This distribution conforms to Hardy-Weinberg predictions for the autosomal codominant inheritance of a pair of alleles for low and high TPMT activity, TPMT(L) and TPMT(H), with gene frequencies of .059 and .941, respectively. If RBC TPMT activity is inherited in an autosomal codominant fashion, then subjects homozygous for TPMT(H) would have high enzyme activity, subjects heterozygous for the two alleles would have intermediate activity, and subjects homozygous for TPMT(L) would have undetectable activity. The segregation of RBC TPMT activity among 215 first-degree relatives in 50 randomly selected families and among 35 members of two kindreds and one family selected because they included probands with undetectable RBC enzyme activity were also compatible with the autosomal codominant inheritance of RBC TPMT. For example, in eight matings between subjects with intermediate activity (presumed genotype TPMT(L)TPMT(H)) and subjects with high activity (presumed genotype TPMT(H)TPMT(H)), 47% (8/17) of the offspring had intermediate activity. This value is very similar to the 50% figure expected on the basis of autosomal codominant inheritance (chi(2) ([1]) = .059). Further experiments are required to determine whether this genetic polymorphism for an important drug metabolizing enzyme may represent one factor in individual variations in sensitivity to thiopurines.
Volatile Rare Earth Chelates (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVolatile Rare Earth ChelatesKent J. Eisentraut and Robert E. SieversCite this: J. Am. Chem. Soc. 1965, 87, 22, 5254–5256Publication Date (Print):November 1, 1965Publication History Published online1 May 2002Published inissue 1 November 1965https://pubs.acs.org/doi/10.1021/ja00950a051https://doi.org/10.1021/ja00950a051research-articleACS PublicationsRequest reuse permissionsArticle Views1277Altmetric-Citations302LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Tissue Engineering by Self-Assembly of Cells Printed into Topologically Defined Structures (2008)

Understanding the principles of biological self-assembly is indispensable for developing efficient strategies to build living tissues and organs. We exploit the self-organizing capacity of cells and tissues to construct functional living structures of prescribed shape. In our technology, multicellular spheroids (bio-ink particles) are placed into biocompatible environment (bio-paper) by the use of a three-dimensional delivery device (bio-printer). Our approach mimics early morphogenesis and is based on the realization that the genetic control of developmental patterning through self-assembly involves physical mechanisms. Three-dimensional tissue structures are formed through the postprinting fusion of the bio-ink particles, in analogy with early structure-forming processes in the embryo that utilize the apparent liquid-like behavior of tissues composed of motile and adhesive cells. We modeled the process of self-assembly by fusion of bio-ink particles, and employed this novel technology to print extended cellular structures of various shapes. Functionality was tested on cardiac constructs built from embryonic cardiac and endothelial cells. The postprinting self-assembly of bio-ink particles resulted in synchronously beating solid tissue blocks, showing signs of early vascularization, with the endothelial cells organized into vessel-like conduits.
Molecular structure of Rarobacter faecitabidus protease I. A yeast-lytic serine protease having mannose-binding activity. (1992)

Rarobacter faecitabidus protease I (RPI) is a serine protease exhibiting lytic activity toward living yeast cells. RPI is similar to elastase in its substrate specificity and has a lectin-like affinity for mannose. The gene encoding RPI was cloned to elucidate its structure and function. And its nucleotide sequence revealed that it contains an open reading frame encoding a 525-amino acid protein. Homology comparison indicated that pre-pro-RPI consists of three domains: (1) an NH2-terminal prepro domain not found in the mature form of RPI, (2) a protease domain homologous to the trypsin family of serine proteases, and (3) a COOH-terminal domain homologous to the COOH-terminal part of Oerskovia xanthineolytica beta-1,3-glucanase and the NH2-terminal part of the ricin B chain, a lectin isolated from the part of the ricin B chain, a lectin isolated from the castor bean. The RPI gene and its mutant were subsequently expressed in Escherichia coli under its beta-galactosidase promoter to investigate the function of the COOH-terminal domain. The mutant RPI, whose COOH-terminal domain was truncated by site-directed mutagenesis, lost both its mannose-binding and yeast-lytic activity, although the protease activity was not affected. These findings suggest that the COOH-terminal domain actually participates in the mannose-binding activity and is required for yeast-lytic activity.
Transfer hydrogenation with Hantzsch esters and related organic hydride donors (2012)

In recent years, Hantzsch esters and their related organic hydride donors have been widely utilized in biomimetic approaches of asymmetric transfer hydrogenation (ATH) reactions. Various compounds containing C=C, C=N and C=O unsaturated functionalities could be reduced in the presence of organocatalysts or transition metal complexes, affording versatile chiral building blocks in high yields and excellent enantioselectivities under mild conditions. In this critical review, recent advances in this area are summarized and classified according to unsaturated functional groups being reduced and catalytic systems employed (91 references).
Assessment of protein coding measures (1992)

A number of methods for recognizing protein coding genes in DNA sequence have been published over the last 13 years, and new, more comprehensive algorithms, drawing on the repertoire of existing techniques, continue to be developed. To optimize continued development, it is valuable to systematically review and evaluate published techniques. At the core of most gene recognition algorithms is one or more coding measures--functions which produce, given any sample window of sequence, a number or vector intended to measure the degree to which a sample sequence resembles a window of 'typical' exonic DNA. In this paper we review and synthesize the underlying coding measures from published algorithms. A standardized benchmark is described, and each of the measures is evaluated according to this benchmark. Our main conclusion is that a very simple and obvious measure--counting oligomers--is more effective than any of the more sophisticated measures. Different measures contain different information. However there is a great deal of redundancy in the current suite of measures. We show that in future development of gene recognition algorithms, attention can probably be limited to six of the twenty or so measures proposed to date.
Valuing the Coast and Marine Environment: Lessons from Wales (2006)

Estimate the economic value of the coast and marine environment in Wales. There can be no doubt that the coast and marine environment makes a substantial contribution to the Welsh economy in terms of jobs, incomes and GDP, and the prosperity of significant parts of Wales is highly dependent on that contribution. At the same time the coast and marine environment is under increasingly competing pressures as a resource. The importance of tourism is notable in the findings reported here and encapsulates the inherent conflict between the increased development of, and sustainability of Wales' coastal and marine assets. One increasingly effective means of managing these tensions is an integrated coastal management strategy that recognises but works round the inevitable tradeoffs.
Analysis and optimization of monolithic inductors and transformers for RF ICs (2002)

A fast and accurate approach for analyzing Si IC spiral inductors and transformers is presented. The technique incorporates the substrate in the calculation to fully characterize the devices. Many test structures are fabricated and measured data is used to verify the analysis technique over a broad frequency range. Suitable lumped broadband equivalent circuit models of the structures are presented which can be incorporated into traditional circuit simulators. A custom CAD tool ASITIC is described which is used for the design and optimization of inductors and transformers.
Incompatibilities of Pharmaceutical Excipients with Active Pharmaceutical Ingredients: A Comprehensive Review (2010)

Studies of drug-excipient compatibility represent an important phase in the pre-formulation stage for the development of all dosage forms. The potential physical and chemical interactions between drugs and excipients can affect the chemical nature, the stability and bioavailability of drugs and, consequently, their therapeutic efficacy and safety. The present review covers the literature reports of incompatibilities of commonly used pharmaceutical excipients with different active pharmaceutical ingredients. Examples of drug-excipient interactions, such as transacylation, the Maillard browning reaction, acid base reactions and physical changes are discussed for different active pharmaceutical ingredients belonging to different therapeutic areas viz. antiviral, anti-inflammatory, antidiabetic, antihypertensives, CNS drugs, anti-convulsants, antibiotics, bronchodialators, antimalarial, amtiemetic, vitamins, antiamoebics, antipsychotics, antidepressants, anticancer, anticoagulants and sedatives/ hypnotics. Once solid-state reactions are understood in a pharmaceutical system, the necessary steps can be taken to prevent reactivity and improve the stability of drug substances and products.
Nitric oxide inhibits Kv4.3 and human cardiac transient outward potassium current (Ito1) (2008)

Our results suggested that the increase in the atrial NO bioavailability could partially restore the duration of the plateau phase of CAF-remodelled AP by inhibiting the I(to1) as a result of the activation of non-canonical enzymatic pathways.
The influence of glucan polymer structure and solution conformation on binding to (1->3)- -D-glucan receptors in a human monocyte-like cell line (2000)

Glucans are (1-3)-beta-D-linked polymers of glucose that are produced as fungal cell wall constituents and are also released into the extracellular milieu. Glucans modulate immune function via macrophage participation. The first step in macrophage activation by (1-3)-beta-D-glucans is thought to be the binding of the polymer to specific macrophage receptors. We examined the binding/uptake of a variety of water soluble (1-3)-beta-D-glucans and control polymers with different physicochemical properties to investigate the relationship between polymer structure and receptor binding in the CR3- human promonocytic cell line, U937. We observed that the U937 receptors were specific for (1-->3)-beta-D-glucan binding, since mannan, dextran, or barley glucan did not bind. Scleroglucan exhibited the highest binding affinity with an IC(50)of 23 nM, three orders of magnitude greater than the other (1-->3)-beta-D-glucan polymers examined. The rank order competitive binding affinities for the glucan polymers were scleroglucan>>>schizophyllan > laminarin > glucan phosphate > glucan sulfate. Scleroglucan also exhibited a triple helical solution structure (nu = 1.82, beta = 0.8). There were two different binding/uptake sites on U937 cells. Glucan phosphate and schizophyllan interacted nonselectively with the two sites. Scleroglucan and glucan sulfate interacted preferentially with one site, while laminarin interacted preferentially with the other site. These data indicate that U937 cells have at least two non-CR3 receptor(s) which specifically interact with (1-->3)-beta-D-glucans and that the triple helical solution conformation, molecular weight and charge of the glucan polymer may be important determinants in receptor ligand interaction.
Rotavirus vaccines: an overview. (1996)

Rotavirus vaccine development has focused on the delivery of live attenuated rotavirus strains by the oral route. The initial "Jennerian" approach involving bovine (RIT4237, WC3) or rhesus (RRV) rotavirus vaccine candidates showed that these vaccines were safe, well tolerated, and immunogenic but induced highly variable rates of protection against rotavirus diarrhea. The goal of a rotavirus vaccine is to prevent severe illness that can lead to dehydration in infants and young children in both developed and developing countries. These studies led to the concept that a multivalent vaccine that represented each of the four epidemiologically important VP7 serotypes might be necessary to induce protection in young infants, the target population for vaccination. Human-animal rotavirus reassortants whose gene encoding VP7 was derived from their human rotavirus parent but whose remaining genes were derived from the animal rotavirus parent were developed as vaccine candidates. The greatest experience with a multivalent vaccine to date has been gained with the quadrivalent preparation containing RRV (VP7 serotype 3) and human-RRV reassortants of VP7 serotype 1, 2, and 4 specificity. Preliminary efficacy trial results in the United States have been promising, whereas a study in Peru has shown only limited protection. Human-bovine reassortant vaccines, including a candidate that contains the VP4 gene of a human rotavirus (VP4 serotype 1A), are also being studied.
Path-based scheduling for synthesis (2002)

A path-based scheduling algorithm for synchronous digital systems is presented. It yields solutions with the minimum number of control steps, taking into account arbitrary constraints that limit the amount of operations in each control step. The result is a finite-state machine that implements the control. Although the complexity of the algorithm is proportional to the number of paths in the control-flow graph, it is shown to be practical for large examples with thousands of nodes.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Plaque Assay for Respiratory Syncytial Virus (1963)

SummaryAn accurate and sensitive method for enumeration of infective plaque forming units of respiratory syncytial virus, utilizing agar-overlaid HEp-2 monolayers in Petri dishes, is described. Small macroscopic plaques were observed 9 days after infection, and attained a final diameter of 2 mm and maximum number by the 11th or 12th day. By use of the technic of plaque reduction, it was possible to detect amounts of neutralizing antibody to RS virus hitherto not measurable by the use of standard endpoint dilution methods.
Direct interaction of ligand–receptor pairs specifying stomatal patterning (2012)

Valves on the plant epidermis called stomata develop according to positional cues, which likely involve putative ligands (EPIDERMAL PATTERNING FACTORS [EPFs]) and putative receptors (ERECTA family receptor kinases and TOO MANY MOUTHS [TMM]) in Arabidopsis. Here we report the direct, robust, and saturable binding of bioactive EPF peptides to the ERECTA family. In contrast, TMM exhibits negligible binding to EPF1 but binding to EPF2. The ERECTA family forms receptor homomers in vivo. On the other hand, TMM associates with the ERECTA family but not with itself. While ERECTA family receptor kinases exhibit complex redundancy, blocking ERECTA and ERECTA-LIKE1 (ERL1) signaling confers specific insensitivity to EPF2 and EPF1, respectively. Our results place the ERECTA family as the primary receptors for EPFs with TMM as a signal modulator and establish EPF2-ERECTA and EPF1-ERL1 as ligand-receptor pairs specifying two steps of stomatal development: initiation and spacing divisions.
Interrogation of fiber grating sensor arrays with a wavelength-swept fiber laser (1998)

We demonstrate a novel application of a wavelength-swept fiber laser to fiber Bragg grating sensor-array interrogation. The laser provides high signal powers of >3 mW with <0.1-nm spectral resolution over a 28-nm wavelength span. Using time-interval counting, we demonstrate static-dynamic strain measurements with a resolution of 0.47mu? rms at a sampling rate of 250 Hz.
Research of camera calibration algorithm based on 3D re-projective error (2009)

According to the calibration of binocular vision, an improved two-stages camera calibration method involved with multi-distortion coefficients is introduced in this paper. It takes the sum of distance in the world coordinate between ground truth 3D points and calculated 3D points as cost function. The 3D points' coordinate are calculated by the imitated direct linear transformation (IDLT) triangulation based on distortion compensation. This strategy can allow the two cameras' all parameters being optimized at the same time. The experiment among IDLT method , Matlab calibration toolbox method and Tsai mehtod is made and the results show that the strategy of taking the 3D reconstruction errors as object function is feasible, and IDLT method possesses more higher calibration precision than those of the other two methods.