gebhart/patrepeval huggingface dataset:
Advances in 3D CMOS sequential integration (2009)

For the first time 3D sequential CMOS integration turns up to be an actual competitor for sub 22nm technology nodes. Thanks to the original use of molecular bonding, high quality top Si active layers are obtained. Thermally robust bottom salicide goes through the whole top FET processing without any significant sheet resistance degradation. The low temperature integration of raised source and drain for top layers is demonstrated. A decrease by 4¿ of the Equivalent Oxide Thickness is measured when a low thermal budget process is implemented. The electrostatic coupling between stacked FETs is demonstrated thanks to an ultra thin inter layer dielectric thickness of 60nm. It leads to a threshold voltage dynamic shift of 130mV enabling SRAM stabilization.
T1 independent, T2* corrected MRI with accurate spectral modeling for quantification of fat: Validation in a fat‐water‐SPIO phantom (2009)

This work demonstrates the necessity of T(2)* correction and accurate spectral modeling of fat to accurately quantify fat using MRI.
Differences in Surface Expression of NspA amongNeisseria meningitidisGroup B Strains (1999)

NspA is a highly conserved membrane protein that is reported to elicit protective antibody responses against Neisseria meningitidis serogroups A, B and C in mice (D. Martin, N. Cadieux, J. Hanel, and B. R. Brodeur, J. Exp. Med. 185:1173-1183, 1997). To investigate the vaccine potential of NspA, we produced mouse anti-recombinant NspA (rNspA) antisera, which were used to evaluate the accessibility of NspA epitopes on the surface of different serogroup B strains by an immunofluorescence flow cytometric assay and by susceptibility to antibody-dependent, complement-mediated bacteriolysis. Among 17 genetically diverse strains tested, 11 (65%) were positive for NspA cell surface epitopes and 6 (35%) were negative. All six negative strains also were resistant to bactericidal activity induced by the anti-rNspA antiserum. In contrast, of the 11 NspA surface-positive strains, 8 (73%; P < 0.05) were killed by the antiserum and complement. In infant rats challenged with one of these eight strains, the anti-rNspA antiserum conferred protection against bacteremia, whereas the antiserum failed to protect rats challenged by one of the six NspA cell surface-negative strains. Neither NspA expression nor protein sequence accounted for differences in NspA surface accessibility, since all six negative strains expressed NspA in outer membrane preparations and since their predicted NspA amino acid sequences were 99 to 100% identical to those of three representative positive strains. However, the six NspA cell surface-negative strains produced, on average, larger amounts of group B polysaccharide than did the 11 positive strains (reciprocal geometric mean titers, 676 and 224, respectively; P < 0.05), which suggests that the capsule may limit the accessibility of NspA surface epitopes. Given these strain differences in NspA surface accessibility, an rNspA-based meningococcal B vaccine may have to be supplemented by additional antigens.
Redesign of soluble fatty acid desaturases from plants for altered substrate specificity and double bond position (1997)

Acyl-acyl carrier protein (ACP) desaturases introduce double bonds at specific positions in fatty acids of defined chain lengths and are one of the major determinants of the monounsaturated fatty acid composition of vegetable oils. Mutagenesis studies were conducted to determine the structural basis for the substrate and double bond positional specificities displayed by acyl-ACP desaturases. By replacement of specific amino acid residues in a Delta6-palmitoyl (16:0)-ACP desaturase with their equivalents from a Delta9-stearoyl (18:0)-ACP desaturase, mutant enzymes were identified that have altered fatty acid chain-length specificities or that can insert double bonds into either the Delta6 or Delta9 positions of 16:0- and 18:0-ACP. Most notably, by replacement of five amino acids (A181T/A200F/S205N/L206T/G207A), the Delta6-16:0-ACP desaturase was converted into an enzyme that functions principally as a Delta9-18:0-ACP desaturase. Many of the determinants of fatty acid chain-length specificity in these mutants are found in residues that line the substrate binding channel as revealed by x-ray crystallography of the Delta9-18:0-ACP desaturase. The crystallographic model of the active site is also consistent with the diverged activities associated with naturally occurring variant acyl-ACP desaturases. In addition, on the basis of the active-site model, a Delta9-18:0-ACP desaturase was converted into an enzyme with substrate preference for 16:0-ACP by replacement of two residues (L118F/P179I). These results demonstrate the ability to rationally modify acyl-ACP desaturase activities through site-directed mutagenesis and represent a first step toward the design of acyl-ACP desaturases for the production of novel monounsaturated fatty acids in transgenic oilseed crops.
Bipolar transistor carrier-injected optical modulator/switch: Proposal and analysis (1986)

Bipolar transistor structures can be used, instead of conventional p-n (p-i-n) junctions, to realize high-speed optical modulators and switches, which are operated by free-carrier injection. Some basic results obtained by the theoretical analysis of the structure applied to a DH X crossing are presented, with emphasis on the switching speed. It is shown that switching times can be as fast as 60 ps for the required ON-OFF optical switching, which is considerably faster than those expected for a diode structure.
Heparan sulfate proteoglycans of human lung fibroblasts. Occurrence of distinct membrane, matrix and secreted forms (1986)

Human lung fibroblasts (HLF) were labeled with 35SO2-4 for 48 h and extracted with a guanidinium chloride buffer. A fraction of the extracted heparan sulfate proteoglycan (HSPG) appeared micelle-associated. In the absence of detergent these HSPG eluted in the void volume of Sepharose CL2B columns. In the presence of detergent these HSPG were included in Sepharose CL2B (Kav = 0.55) and 4B (Kav = 0.3) columns. This type of HSPG was specifically associated with isolated HLF cell membranes, suggesting that it may represent a fraction of integral membrane proteoglycans. Most of the HSPG in the HLF monolayers, however, eluted in the included volume of Sepharose CL2B (Kav = 0.4) and CL4B (Kav = 0.1) columns in the absence of detergent. This type of HSPG was not affected by detergent and was specifically retained in 'extracellular matrix' preparations. The medium of HLF monolayers contained HSPG of similar Mr as the membrane-associated HSPG. Of these three distinct HSPG fractions only the membrane-associated form could be incorporated in liposomes, confirming that the HSPG in this fraction may be integral membrane components.
The burden and outcomes associated with four leukemias: AML, ALL, CLL and CML (2003)

Given the recent advances in the treatment of hematologic malignancies and the many other treatments on the horizon, physicians and payers will be faced with the critical decisions of when to use new treatments in the clinical pathway and how to allocate healthcare resources. This review will provide an overall context for the clinical, economic and quality of life burden of leukemia, as well as provide cross-analysis among the four major types of leukemia: acute lymphocytic leukemia, chronic lymphocytic leukemia, acute myeloid leukemia and chronic myeloid leukemia.
Pharmaceutical, cellular and genetic therapies for Huntington's disease (2005)

HD (Huntington's disease) is a devastating neurodegenerative disorder caused by a polyglutamine expansion in the gene encoding the huntingtin protein. Presently, there is no known cure for HD and existing symptomatic treatments are limited. However, recent advances have identified multiple pathological mechanisms involved in HD, some of which have now become the focus of therapeutic intervention. In this review, we consider progress made towards developing safe and effective pharmaceutical-, cell- and genetic-based therapies, and discuss the extent to which some of these therapies have been successfully translated into clinical trials. These new prospects offer hope for delaying and possibly halting this debilitating disease.
Establishment of conditional vectors for hairpin siRNA knockdowns (2003)

Small interference RNA (siRNA) is an emerging methodology in reverse genetics. Here we report the development of a new tetracycline-inducible vector-based siRNA system, which uses a tetracycline-responsive derivative of the U6 promoter and the tetracycline repressor for conditional in vivo transcription of short hairpin RNA. This method prevents potential lethality immediately after transfection of a vector when the targeted gene is indispensable, or the phenotype of the knockdown is lethal or results in a growth abnormality. We show that the controlled knockdown of DNA methyltransferase 1 (DNMT1) in human cancer resulted in growth arrest. Removal of the inducer, doxycycline, from treated cells led to re-expression of the targeted gene. Thus the method allows for a highly controlled approach to gene knockdown.
Internal association in solid phase peptide synthesis. Synthesis of cytochrome C residues 66–104 on polyamide supports (1980)

E. Atherton, V. Woolley and R. C. Sheppard, J. Chem. Soc., Chem. Commun., 1980, 970 DOI: 10.1039/C39800000970
Secondary immunity to Legionella pneumophila and Th1 activity are suppressed by delta-9-tetrahydrocannabinol injection (1994)

Resistance to infection with Legionella pneumophila is primarily dependent upon cell-mediated immunity rather than humoral immunity. Recent evidence suggests that activation of cell-mediated immunity depends on Th1 cells and activation of humoral immunity depends on Th2 cells. In this report, delta 9-tetrahydrocannabinol (THC), the major psychoactive cannabinoid of marijuana and an immunomodulator, suppressed development of secondary immunity to L. pneumophila, which correlated with a reduction in Th1 activity. BALB/c mice, infected with a primary sublethal dose of L. pneumophila, developed resistance to a larger challenge infection 3 to 4 weeks later. However, intravenous injection of THC (4 mg/kg of body weight) 1 day prior to primary infection resulted in increased mortality after the challenge infection. The level of anti-L. pneumophila antibodies in serum increased in both THC-treated and control mice; however, in the THC group IgG1 antibodies which are stimulated by Th2 cells were elevated while Th1-regulated, IgG2a antibodies were depressed. Furthermore, cultured splenocytes from THC-treated mice had less L. pneumophila-specific lymphoproliferation, indicating a deficiency in cell-mediated immunity. Normal mouse splenocytes treated in vitro with THC and pokeweed mitogen showed suppressed production of gamma interferon, a cytokine associated with Th1 cells, but increased production of interleukin 4, a cytokine produced by Th2 cells. Splenocytes from THC-treated mice, stimulated in vitro with either pokeweed mitogen or anti-CD3 antibodies, also produced less gamma interferon, indicating less Th1 activity in these mice. These results suggest that THC decreases the development of anti-L. pneumophila immunity by causing a change in the balance of Th1 and Th2 activities.
Intensification by Intrauterine Devices of Sperm Loss from the Sheep Uterus1 (1970)

A plastic spiral (IUD) was inserted by surgery into one uterine horn of 105 parous ewes; 22 other ewes were sham-operated on. About 8 weeks later, each uterine horn was ligated, and 0.07 ml of fresh ram semen was deposited in the lumen. The horns were flushed 1 or 5 hr later, and intact sperm and tailless sperm heads were counted. Sperm cell loss was greater in sham-operated estrous and ovariectomized than in shamoperated luteal phase ewes, suggesting that progesterone decreased the rate of sperm loss. The presence of an IUD greatly intensified the loss of sperm in ewes of each endocrine state. Washing accumulated cellular debris from the lumens of IUD horns before depositing semen did not reduce the loss of sperm. Excising the uterus just before depositing semen largely prevented sperm loss. These results suggest that intensified sperm loss in IUD horns was caused by a change in uterine function induced by the presence of semen and not by factors already present in the uterine lumen.
[Enzymatic reactions in biotechnology (48th Bach lecture)]. (1993)

The paper is the 48th Bach Lecture presented under the same title. It covers the biochemical mechanisms of the biogenesis of microbial biosynthetic products, role of acetyl-CoA, function of the succinate-glycine cycle, reactions of the hexose-monophosphate pathway of carbon metabolism. The reversible action of hydrolases in enzymatic catalysis and degradation of xenobiotics are discussed. The data on redox reactions are pooled. Such modern biotechnological processes as epoxidation, synthesis of acrylamide and some monomers involved in chemical syntheses of polymers, synthesis of oligosaccharide and fluorine-containing amino acids are considered. Promising commercial applications of biocatalysis are discussed.
<title>Medical linear accelerator x-ray sources: variation with make, model, and time</title> (1992)

One difficulty in radiation therapy is ensuring the correct placement of the radiation field so that the radiation is delivered to the diseased regions while healthy tissues are spared. Currently, field placement is assessed by producing a transmission radiograph (portal image) using the high-energy treatment beam. Often the quality of these images is poor. One factor which influences image quality is the size of the x-ray source found in medical linear accelerators. These sources can be large and thus reduce the spatial resolution of the portal images, thereby reducing our ability to detect bony anatomical landmarks. The high energy of the x rays generated in these accelerators make it impossible to measure the x-ray source using standard techniques (e.g., pin-hole camera or star-patterns). We have developed a reconstruction technique which allows quantitative measurement of these sources. Using this technique, we have investigated and compared the size and shape of x-ray sources on a total of seven accelerators. These include: (1) two Varian Clinac 2100cs, (2) two Atomic Energy of Canada Ltd. (AECL) Therac 6s, (3) a Varian Clinac 600c, (4) an AECL Therac 25, and (5) a Therac 20. The comparisons also include monitoring the size and shape of the sources over a two year period. For each of these measured sources the source MTF has been calculated at typical magnifications. It has been found that (1) the size and shape of the source spot varies greatly between accelerators of different design; (2) for accelerators of the same design, however, the source spots were similar; and (3) the spatial resolution of currently available on-line verification imaging systems is only marginally reduced by the size and shape these source spots.
A 128×128 CMOS active pixel image sensor for highly integrated imaging systems (2002)

A new CMOS-based image sensor that is intrinsically compatible with on-chip CMOS circuitry is reported. The new CMOS active pixel image sensor achieves low noise, high sensitivity, X-Y addressability, and has simple timing requirements. The image sensor was fabricated using a 2 /spl mu/m p-well CMOS process, and consists of a 128/spl times/128 array of 40 /spl mu/m/spl times/40 /spl mu/m pixels. The CMOS image sensor technology enables highly integrated smart image sensors, and makes the design, incorporation and fabrication of such sensors widely accessible to the integrated circuit community.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Metabotropic Glutamate Receptor Involvement in Models of Acute and Persistent Pain: Prospects for the Development of Novel Analgesics (2002)

The excitatory amino acid glutamate plays a major role in nociceptive processing. Ionotropic and metabotropic glutamate receptors are expressed in relevant areas of the brain, spinal cord and periphery that are involved in pain sensation and transmission. Activation of mGlu receptors along the pain neuraxis can result in either pronociceptive or antinociceptive behaviors depending on the subtype of mGluR and its location. The data published to date most strongly support the idea that mGlu1 antagonists might act as broad-spectrum analgesics. Several studies pointing to a functional upregulation of mGlu2/3 in chronic pain models suggest that agonists of these receptors might also be effective analgesics in certain conditions, most notably inflammation-induced hyperalgesia and allodynia. The expression of mGluRs throughout the pain neuraxis and the differing roles of the mGluRs in each of these regions makes it difficult to predict the efficacy of mGluR ligands based on in vitro or local administration studies. Potent, systemically active compounds that show mGluR subtype selectivity will be critical to undertake more detailed analyses in animal models of pain.
The TCR zeta-chain immunoreceptor tyrosine-based activation motifs are sufficient for the activation and differentiation of primary T lymphocytes. (1999)

The TCR complex signals through a set of 10 intracytoplasmic motifs, termed immunoreceptor tyrosine-based activation motifs (ITAMs), contained within the gamma-, delta-, epsilon-, and zeta-chains. The need for this number of ITAMs is uncertain. Limited and contradictory studies have examined the ability of subsets of the TCR's ITAMs to signal into postthymic primary T lymphocytes. To study signaling by a restricted set of ITAMs, we expressed in transgenic mice a chimeric construct containing the IAs class II MHC extracellular and transmembrane domains linked to the cytoplasmic domain of the TCR zeta-chain. Tyrosine phosphorylation and receptor cocapping studies indicate that this chimeric receptor signals T cells independently of the remainder of the TCR. We show that CD4+ and CD8+ primary T cells, as well as naive and memory T cells, are fully responsive to stimulation through the IAs-zeta receptor. Further, IAs-zeta stimulation can induce primary T cell differentiation into CTL, Th1, and Th2 type cells. These results show that the zeta-chain ITAMs, in the absence of the gamma, delta, and epsilon ITAMs, are sufficient for the activation and functional maturation of primary T lymphocytes. It also supports the isolated use of the zeta-chain ITAMs in the development of surrogate TCRs for therapeutic purposes.
Human Divinity (2005)

Against the background of the Old Testament's lack of unambiguousness regarding the divine nature of compassion and the narrative's latent call to surmount human compassion and its dangers, the Judeo-Christian traditions of the post-biblical period weave a more complex and intricate picture. It may well have been the dramatic appearance of Jesus, the story of his crucifixion, the debates over his divinity, and the persecution of Christians in the first few centuries of the Common Era that laid the groundwork for the search for a tangible and concrete manifestation of compassion in human existence. Both in Christianity and in Judaism, figures began to appear, whose lives and actions embodied compassion, endowing them with the special status of good, pure people, and, later on, saints. In Christianity, these were people directly connected to Jesus — the apostles, the martyrs, and, especially, Mary, who, in the evolving world of Christian imagery, received the unofficial status of representative of all-embracing, unconditional, all-forgiving maternal compassion.KeywordsDivine NatureDivine LoveHoly GhostChristian ConceptDivine GraceThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Ectopic Expression of the Murine Chemokines CCL21a and CCL21b Induces the Formation of Lymph Node-Like Structures in Pancreas, But Not Skin, of Transgenic Mice (2002)

The CC chemokine CCL21 is a potent chemoattractant for lymphocytes and dendritic cells in vitro. In the murine genome there are multiple copies of CCL21 encoding two CCL21 proteins that differ from each other by one amino acid at position 65 (either a serine or leucine residue). In this report, we examine the expression pattern and biological activities of both forms of CCL21. We found that although both serine and leucine forms are expressed in most tissues examined, the former was the predominant form in lymphoid organs while the latter was predominantly expressed in nonlymphoid organs. When expressed in transgenic pancreas, both forms of CCL21 were capable of inducing the formation of lymph node-like structures composed primarily of T and B cells and a few dendritic cells. Induction of lymph node-like structures by these CCL21 proteins, however, could not be reproduced in every tissue. For instance, no lymphocyte recruitment or accumulation was observed when CCL21 was overexpressed in the skin. We conclude that both forms of CCL21 protein are biologically equivalent in promoting lymphocyte recruitment to the pancreas, and that their ability to induce the formation of lymph node-like structures is dependent on the tissues in which they are expressed.
Membrane receptors of mouse leukocytes. II. Sequential expression of membrane receptors and phagocytic capacity during leukocyte differentiation (1978)

Analysis of four mature cell markers on mouse bone marrow leukocytes grown in vitro, demonstrated a distinct sequence of marker appearance during the terminal phases of granulocytic cell differentiation. A similar pattern of marker expression was also suggested by analysis of mature neutrophils and macrophages isolated from normal tissues. Among cultured neutrophils, receptors for the Fc portion of IgG (FcR) were first expressed on myelocytes and metamyelocytes, and then subsequently on more mature cells. Morphologically mature colony neutrophils (polymorphs) from agar cultures contained only FcR and complement receptor type two (CR(2)) (C3d receptor), and lacked both complement receptor type one (CR(1)) (C3b receptor) and the capacity to ingest latex, bacteria, or iron particles. Neutrophils from 2 and 3 wk liquid media cultures of marrow cells differed from agar grown neutrophils in that they had phagocytic capacity (particle ingestion) [Pi] in addition to FcR and CR(2). Furthermore, in the 4th and 5th wk of these continuous liquid cultures, CR(1) was also expressed, completing the surface marker profile of normal blood neutrophils. Based on these studies, the following order of appearance of these four markers on cells from the myelocytic series was proposed: FcR {arrow} FcR CR(2) {arrow} FcR CR(2) Pi {arrow} FcR CR(2) Pi CR(1). Differential studies of tissue leukocytes containing these same markers revealed that a heterogeneity existed among morphologically mature neutrophils. Even though 95 percent of blood polymorphs contained all four markers, the same was true of only half of spleen polymorphs and only 20 percent of bone marrow polymorphs. Cells of the monocyte-macrophage series were studies in parallel with neutrophils. Cultured marrow monocytes acquired the four mature cell markers so rapidly that the order of receptor appearance could not be determined. However, it was found that CR2 was lost during the terminal phase of monocyte maturation into activated macrophages.