gebhart/patrepeval huggingface dataset:
Efficient radially polarized laser beam generation with a double interferometer (1993)

Conversion of a linearly polarized CO(2) laser beam into a radially polarized beam is demonstrated with a novel double-interferometer system. The first Mach-Zehnder interferometer converts the linearly polarized input beam into two beams with sin(2) θ and cos(2) θ intensity profiles, where θ is the azimuthal angle. This is accomplished by using two spiral-phase-delay plates with opposite handedness in the two legs of the interferometer to impart a one-wave phase delay azimuthally across the face of the beams. After these beams are interfered with, the resulting beams are sent directly into the second Mach-Zehnder interferometer, where the polarization direction of one beam is rotated by 90°. The beams are then recombined at the output of the second interferometer with a polarization-sensitive beam splitter to generate a radially polarized beam. The output beam is ≈92% radially polarized and contains ≈85% of the input power. This system will be used in upcoming laser particle acceleration experiments.
Involvement of kinesin family member 2C/mitotic centromere‐associated kinesin overexpression in mammary carcinogenesis (2007)

To elucidate the molecular mechanisms of mammary carcinogenesis and discover novel therapeutic targets for breast cancer, we previously carried out genome-wide expression profile analysis of 81 breast cancer cases by means of cDNA microarray coupled with laser microbeam microdissection of cancer cells. Among the dozens of transactivated genes, in the present study we focused on the functional significance of kinesin family member 2C (KIF2C)/mitotic centromere-associated kinesin (MCAK) in the growth of breast cancer cells. Northern blot and immunohistochemical analyses confirmed KIF2C/MCAK overexpression in breast cancer cells, and showed that it is expressed at undetectable levels in normal human tissues except the testis, suggesting KIF2C/MCAK to be a cancer-testis antigen. Western blot analysis using breast cancer cell lines revealed a significant increase in the endogenous KIF2C/MCAK protein level and its phosphorylation in G(2)/M phase. Treatment of breast cancer cells with small interfering RNA against KIF2C/MCAK effectively suppressed KIF2C/MCAK expression and inhibited the growth of the breast cancer cell lines T47D and HBC5. In addition, we found that KIF2C/MCAK expression was significantly suppressed by ectopic introduction of p53. These findings suggest that overexpression of KIF2C/MCAK might be involved in breast carcinogenesis and is a promising therapeutic target for breast cancers.
Angiopoietin-4 Inhibits Angiogenesis and Reduces Interstitial Fluid Pressure (2006)

Angiopoietins (Ang) are involved in the remodeling, maturation, and stabilization of the vascular network. Ang-4 was discovered more recently; thus, its effect on angiogenesis and its interplay with other angiogenic factors have not been equivocally established. The role of Ang-4 in angiogenesis was tested in Matrigel chambers implanted into the subcutaneous space of nude mice. Ang-4 inhibited the angiogenic response of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and GLC19 tumor cells. In Matrigel chambers with Ang-4-transfected cells, the mean response was significantly lower than that of mock cells. Subcutaneous tumor interstitial fluid pressure (IFP) was significantly lower in Ang-4-transfected GLC19 tumors than in mock-transfected tumors. IFP reduction in Ang-4-transfected tumors was comparable to the reduction seen after bevacizumab treatment. In vitro, we examined the effect of recombinant Ang-4 on endothelial cell migration in Boyden chambers. Human umbilical vein endothelial cell (HUVEC) migration induced by bFGF and VEGF was inhibited by Ang-4 to control levels. In conclusion, we show that rhAng-4, as well as transfection with Ang-4, inhibits angiogenesis induced by GLC19 tumor cells and that Ang-4 expression reduces elevated tumor IFP. In addition, we demonstrate that rhAng-4 inhibits HUVEC migration and growth factor-induced angiogenesis.
Intrinsic Broad-Band White-Light Emission by a Tuned, Corrugated Metal–Organic Framework (2012)

Herein we report on the broad-band direct white-light originating from a single component emitter, namely a novel three-periodic metal-organic framework (MOF). This material features an unprecedented topology with (3,4)-connected nodes. The structure-function relationship in this system is driven by two complementary unique structural features: corrugation and interpenetration. Good correlation between simulated and experimental emission spectra has been attained, resulting in optimized color properties that approach requirements for solid-state lighting (SSL). Guided by the optimized calculated spectra, the tunability of the assembly was proven by the successful in-framework co-doping of Eu(3+). This resulted in significantly improved color properties, opening new paths for the rational design of alternative materials for SSL applications.
Genes Coding for a New Pathway of Aerobic Benzoate Metabolism in Azoarcus evansii (2002)

A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thioesters. The genes coding for this benzoate-induced pathway were investigated in the beta-proteobacterium A. evansii. They were identified on the basis of N-terminal amino acid sequences of purified benzoate metabolic enzymes and of benzoate-induced proteins identified on two-dimensional gels. Fifteen genes probably coding for the benzoate pathway were found to be clustered on the chromosome. These genes code for the following functions: a putative ATP-dependent benzoate transport system, benzoate-CoA ligase, a putative benzoyl-CoA oxygenase, a putative isomerizing enzyme, a putative ring-opening enzyme, enzymes for beta-oxidation of CoA-activated intermediates, thioesterase, and lactone hydrolase, as well as completely unknown enzymes belonging to new protein families. An unusual putative regulator protein consists of a regulator protein and a shikimate kinase I-type domain. A deletion mutant with a deletion in one gene (boxA) was unable to grow with benzoate as the sole organic substrate, but it was able to grow with 3-hydroxybenzoate and adipate. The data support the proposed pathway, which postulates operation of a new type of ring-hydroxylating dioxygenase acting on benzoyl-CoA and nonoxygenolytic ring cleavage. A beta-oxidation-like metabolism of the ring cleavage product is thought to lead to 3-ketoadipyl-CoA, which finally is cleaved into succinyl-CoA and acetyl-CoA.
Cloning and disruption of the PpURA5 gene and construction of a set of integration vectors for the stable genetic modification of Pichia pastoris (2003)

A pair of degenerate primers was used for amplification and cloning of a DNA fragment containing parts of the P. pastoris URA5 and SEC65 genes. Using additional information from a partial genomic sequence of P. pastoris, we cloned and sequenced a 1.9 kb chromosomal fragment containing the complete orotate-phosphoribosyltransferase-encoding URA5 gene. A disruption cassette was constructed by replacing a small part of the open reading frame with a kanamycin-resistance gene. The P. pastoris wild-type strain NRRL Y-11430 was transformed with the disruption cassette and an ura5 auxotrophic strain was identified. To generate marker constructs that can be reused in successive transformations of a single strain, we constructed two lacZ-PpURA3-lacZ and lacZ-PpURA5-lacZ cassettes and used them to disrupt PpOCH1. The PpURA3 and PpURA5 genes in the disruptants were then successfully recycled by selecting for resistance to 5'-fluoro-orotic acid. We also assembled a set of modular plasmids that can be used for the stable genetic modification of P. pastoris via a double cross-over event. The sequence presented here has been submitted to the EMBL data library under Accession No. AY303544.
Familial pain syndromes from mutations of the Nav1.7 sodium channel (2009)

The literature currently suggests that voltage-gated sodium channels play a major role in the pathogenesis of neuropathic pain. Alterations in the expression and targeting of specific sodium channels within injured dorsal root ganglia neurons appear to predispose the neurons to abnormal firing properties, allowing for the development of neuropathic pain. Mutations of one particular sodium channel (Na(v)1.7) have been shown to cause inherited neuropathic pain in humans, specifically in erythromelalgia and paroxysmal extreme pain disorder. Inherited erythromelalgia is the first human pain syndrome to be understood at a molecular level, having been linked to gain-of-function mutations of Na(v)1.7. Conversely, a loss-of-function of the Na(v)1.7 channel can produce channelopathy-associated insensitivity to pain. Therefore, the Na(v)1.7 channel may provide a unique target for the pharmacotherapy of pain in humans. In this review article we summarize current knowledge regarding several different disease manifestations arising from changes within the Na(v)1.7 channel.
Data Structures for Statistical Computing in Python (2010)

In this paper we are concerned with the practical issues of working with data sets common to finance, statistics, and other related fields. pandas is a new library which aims to facilitate working with these data sets and to provide a set of fundamental building blocks for implementing statistical models. We will discuss specific design issues encountered in the course of developing pandas with relevant examples and some comparisons with the R language. We conclude by discussing possible future directions for statistical computing and data analysis using Python.
Achievable information rates on digital subscriber loops: limiting information rates with crosstalk noise (1992)

The capacity and cutoff rates for channels with linear intersymbol interference, power dependent crosstalk noise, and additive white noise are examined, focusing on high speed digital subscriber line data transmission. The effects of varying the level of additive white noise, crosstalk coupling gain, sampling rate, and input power levels are studied in detail for a set of simulated two-wire local loops. A closed-form expression for the shell constrained Gaussian cutoff rate on the crosstalk limited channel is developed and related to the capacity, showing that the relationship between these two rates is the same as on a channel without crosstalk noise. The study also projects achievable rates on a digital subscriber line, inside and outside of a carrier serving area, with a sophisticated but realizable receiver.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The Effect of Ti-Rich TiN Film on Thermal Stability of Ge2Sb2Te5for Phase Change Memory (2016)

Investigation of the thermal stability of Ge2Sb2Te5 (GST) layer in contact with TiN film is very valuable for phase-change memory applications. In this work, the effect of Ti/N atomic ratio in TiN film on thermal stability of GST film has been thoroughly analyzed. After annealing at 410°C for 30 min, the Ti-rich TiN thin film is found to interact with GST material but there does not exist any mixing of Ti, Sb and Te atomic species at the interface between the stoichiometric TiN film and GST, which indicates the stoichiometric TiN film is stable against GST. A Ti-rich TiN film has Ti phase and other TiN compound phases. The presence of the Ti atoms promotes the mutual diffusion and interaction of Ti, Sb and Te under thermal annealing. To ensure the thermal stability of GST in contact with TiN and the performance of device, the Ti/N atomic ratio should be given more attention in the fabrication of PCM devices. Clear boundary and normal operation character are achieved in the T-shaped phase change memory devices with TiN0.6 film.
The new potent immunosuppressant FK-506 reverses multidrug resistance in Chinese hamster ovary cells. (1991)

The macrolide FK-506, produced by Streptomyces tsukubaensis, is effective in sensitizing a multidrug resistant strain of Chinese hamster ovary cells to the cytotoxic action of vinblastine. FK-506 also promotes Adriamycin accumulation into both wild type and resistant cell lines. FK-506 may be a useful drug to enhance the effectiveness of anti-tumour cytotoxic drugs.
Anti‐CD3/28 mediated expansion of macaque CD4+ T cells is polyclonal and provides extended survival after adoptive transfer (2007)

In summary, the data suggest that anti-CD3/28 mediated expansion of CD4(+) T cells retains its immunotherapeutic potential not only during the early stages of lentiviral infection but also at more advanced stages of disease.
Effects of chitosan, pectic acid, lysozyme, and chitinase on the growth of several phytopathogens. (1989)

Chitosan [(l ->4)-linked 2-amino-2-deoxy-/?-D-glucan] and chitin (N-acetylchitosan) are present in the cell walls of phytopathogens.
Overexpression of the shortest isoform of histone demethylase LSD1 primes hematopoietic stem cells for malignant transformation (2015)

Recent investigations indicate that epigenetic regulators act at the initial step of myeloid leukemogenesis by forming preleukemic hematopoietic stem cells (HSCs), which possess the increased self-renewal potential but retain multidifferentiation ability, and synergize with genetic abnormalities in later stages to develop full-blown acute myeloid leukemias. However, it is still unknown whether this theory is applicable to other malignancies. In this study, we demonstrate that lysine-specific demethylase 1 (LSD1) overexpression is a founder abnormality for the development of T-cell lymphoblastic leukemia/lymphoma (T-LBL) using LSD1 transgenic mice. LSD1 expression is tightly regulated via alternative splicing and transcriptional repression in HSCs and is altered in most leukemias, especially T-LBL. Overexpression of the shortest isoform of LSD1, which is specifically repressed in quiescent HSCs and demethylates histone H3K9 more efficiently than other isoforms, increases self-renewal potential via upregulation of the HoxA family but retains multidifferentiation ability with a skewed differentiation to T-cell lineages at transcriptome levels in HSCs. Transgenic mice overexpressing LSD1 in HSCs did not show obvious abnormalities but developed T-LBL at very high frequency after γ-irradiation. LSD1 overexpression appears to be the first hit in T-cell leukemogenesis and provides an insight into novel strategies for early diagnosis and effective treatment of the disease.
Identification and Development of Fucosylated Glycoproteins as Biomarkers of Primary Hepatocellular Carcinoma (2008)

Changes in N-linked glycosylation are known to occur during the development of cancer. For example, we have previously reported changes in N-linked glycosylation that occur with the development of hepatocellular carcinoma (HCC) and, through the use of glycoproteomics, identified many of those proteins containing altered glycan structures. To advance these studies and further explore the glycoproteome, we performed N-linked glycan analysis from serum samples depleted of the major acute phase proteins, followed by targeted lectin extraction of those proteins containing changes in glycosylation. Using this method, changes in glycosylation, specifically increased amounts of core and outer arm fucosylation, were observed in the depleted samples. The identities of those proteins containing core and outer arm fucose were identified in the serum of patients with HCC. The usefulness of some of these proteins in the diagnosis of HCC was determined through the analysis of over 300 patient samples using a high-throughput plate based approach. Greatest performance was achieved with fucosylated hemopexin, which had an AUROC of 0.9515 with an optimal sensitivity of 92% and a specificity of 92%.
SULPHATE-REDUCING BACTERIA AND ANAEROBIC CORROSION (1985)

INTRODUCTION 195 CORROSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196 Electrochemical Mechanism . . . . . . . . . ....... ...... . . . . . . . . . . . . . ....... ... 196 Microbial Corrosion . . . . . . . .. .... . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . 197 Sulphate-Reducing Bacteria and Anaerobic Corrosion. . . . . . . . . . 199 Proposed Mechanisms for Anaerobic Corrosion . .. . . . . . . . . .. . .. ..... . . . . . . . . . . . ...... . . . . . . 200 Anaerobic Corrosion and Complex Ecosystems .. .. ....... . . . . . . . . . . . . . ........ 205
Giving Language Skills a Boost (1996)

Children who are severely impaired in oral language, it seems, are unable to recognize the very short duration sounds of speech. Based on that idea, researchers have now developed a training program that uses computer games to improve the children's ability to understand and respond to spoken language. (The results are also described in Reports on pp. 77 and 81 .) The researchers suggest that similar training may improve the children's reading skills, and perhaps those of the larger population of children who have dyslexia, although that idea is controversial.
Full-rate full-diversity complex-field space-time codes for frequency- or time-selective fading channels (2003)

Multi-antenna systems allow for a significant increase in transmission rates of coherent wireless transmissions over Rayleigh fading channels. Various multi-antenna designs have been developed in recent years targeting either high-performance or high rate. We design a layered space-time (ST) scheme equipped with linear complex field (LCF) coding, which enables full diversity with full rate, for any number of transmit- and receive-antennas through either flat or non-flat fading channels. Our theoretical claims are confirmed by simulations.
Protein−Nanoparticle Interactions: Opportunities and Challenges (2011)

The significant role of protein nanoparticle interactions in nanomedicine and nanotoxicity is emerging recently through the identification of the nanoparticles (NP) protein (biomolecule) corona. The dynamic layer of proteins and/or other biomolecules adsorbed to the nanoparticle surface determines how a NP interacts with living systems and thereby modifies the cellular responses to the NP. Ehrenberg and co-workers used cultured endothelium cells as a model for vascular transport of polystyrene NP with various functional groups, which showed that the capacity of the various NP surfaces to adsorb proteins was indicative of their tendency to associate with cells. The quantification of the adsorbed proteins showed that high-binding NP were maximally coated within seconds to minutes, indicating that proteins on the surface of NP could mediate cell association over much longer time scales. The adsorption or covalent binding of a protein onto a NP's surface can strongly alter the physio-chemical and structural properties of both of them.
Deciphering the Rules of Protein Folding (1989)

RETURN TO ISSUEPREVNewsNEXTDeciphering the Rules of Protein FoldingJONATHAN KINGView Author Information Massachusetts Institute of TechnologyCite this: Chem. Eng. News 1989, 67, 15, 32–54Publication Date (Print):April 10, 1989Publication History Published online10 November 2010Published inissue 10 April 1989https://pubs.acs.org/doi/10.1021/cen-v067n015.p032https://doi.org/10.1021/cen-v067n015.p032newsACS PublicationsCopyright © 1989 American Chemical SocietyArticle Views906Altmetric-Citations52LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Peptides and proteins Get e-Alerts
Clinical comparison of the nitrosoureas (1975)

The nitrosourea group of antitumor agents was developed by the Division of Cancer Treatment of the National Cancer Institute. Three nitrosoureas (BCNU, CCNU, MeCCNU) have undergone extensive clinical trials, and two of them (BCNU, CCNU) will soon become commercially available. This paper briefly considers the preclinical development of the nitrosoureas and provides an overview and comparison of the extensive clinical data. Information is given regarding any clinical differences. We discuss general conclusions drawn from the nitrosourea development.
Biological characterization of folic acid-conjugated poly(H2NPEGCA-co-HDCA) nanoparticles in cellular models (2007)

The folate receptor (FR) is a highly selective tumor marker over expressed in many human cancers and it constitutes a useful target for tumor-specific drug delivery. Thus, the conjugation of folic acid to different drugs or drug carriers may enhance the delivery of the therapeutic agent to FR-positive tumor cells. The aim of this study was to investigate the interactions of folate-conjugated polyalkylcyanoacrylate nanoparticles with tumor cells overexpressing the FR. For this purpose, nanoparticles were prepared by nanoprecipitation of the poly[aminopoly(ethylene glycol) cyanoacrylate-co-hexadecyl cyanoacrylate] [poly(H2NPEGCA-co-HDCA)] copolymer and labeled with the hydrophobic fluorescent dye nile red. Nile red-loaded nanoparticles were then conjugated to folic acid via the PEG terminal amino groups. Four human cancer cell lines were then tested by western blot in order to evaluate the FR expression levels. KB3-1 cell line showed the higher expression level, while MCF-7 cells were taken as a control. After measuring the cytotoxicity of the nanoparticles on these two cell lines, fluorescent folate-nanoparticles were incubated with them and the cellular uptake was evaluated by confocal microscopy and flow cytometry. KB3-1 cells showed a greater nanoparticle internalization, when compared to MCF-7 cells.