gebhart/patrepeval huggingface dataset:
Cytokine mRNA Profile of Myelin Basic Protein Reactive T-cell Clones in Patients with Multiple Sclerosis (1998)

Autoimmune mechanisms involving T-cell responses to (a) myelin autoantigen(s), such as myelin basic protein (MBP), are thought to contribute to the pathogenesis of multiple sclerosis (MS). Cytokines may play a central role in the regulation of the pathogenic autoimmune responses in MS and the mediation of tissue damage in the disease. To study the cytokine expression of myelin reactive T-cells in MS, we determined the cytokine mRNA levels in a panel of blood derived MBP-specific T-cell clones derived from MS patients (33 clones) and normal controls (21 clones), using a novel quantitative RT-PCR method. Our results demonstrate that MBP-specific T-cells, both from MS patients and control subjects, predominantly display a Th1- or Th0-like cytokine pattern. Although MS clones express higher levels of TNFalpha and IL-10 mRNA, these differences do not reach statistical significance. Interestingly, significantly increased TNFalpha and IFNgamma mRNA levels were observed among clones derived from HLA-DR2 positive versus HLA-DR2 negative MS patients. This HLA halpotype is known to be associated with MS. The high levels of TNFalpha and IFNgamma mRNA observed in MBP-reactive T-cell clones from MS patients indicate an important role of these cytokines in the disease process. Our data lend further support to the pathogenic role of MBP-reactive T-cells in MS.
The new drilling control and monitoring system (2004)

The new Drilling control and monitoring system, DrillView, is used on a number of new drilling units in the North Sea and in the Caspian sea. The DrillView system is a modular system that can be used on any size drilling unit, from a single portable top drive to a complete drilling unit with automated pipe handling, cement system, mud system and third party equipment. The Human Man Interface (HMI) is based on touch screen technology (ATEX approved where required). The communication network is based on Ethernet and Profibus, giving full remote operation and monitoring functionality.
NTU RGB+D: A Large Scale Dataset for 3D Human Activity Analysis (2016)

Recent approaches in depth-based human activity analysis achieved outstanding performance and proved the effectiveness of 3D representation for classification of action classes. Currently available depth-based and RGB+D-based action recognition benchmarks have a number of limitations, including the lack of training samples, distinct class labels, camera views and variety of subjects. In this paper we introduce a large-scale dataset for RGB+D human action recognition with more than 56 thousand video samples and 4 million frames, collected from 40 distinct subjects. Our dataset contains 60 different action classes including daily, mutual, and health-related actions. In addition, we propose a new recurrent neural network structure to model the long-term temporal correlation of the features for each body part, and utilize them for better action classification. Experimental results show the advantages of applying deep learning methods over state-of-the-art hand-crafted features on the suggested cross-subject and cross-view evaluation criteria for our dataset. The introduction of this large scale dataset will enable the community to apply, develop and adapt various data-hungry learning techniques for the task of depth-based and RGB+D-based human activity analysis.
Data Warehousing: Concepts, Technologies, Implementations, and Management (1997)

The basics technologies decision support technologies building and management checklist - critical success factors common pitfalls.
Treatment of Vancomycin-Resistant Enterococcus faecium with RP 59500 (Quinupristin-Dalfopristin) Administered by Intermittent or Continuous Infusion, Alone or in Combination with Doxycycline, in an In Vitro Pharmacodynamic Infection Model with Simulated Endocardial Vegetations (1998)

ABSTRACT Quinupristin-dalfopristin is a streptogramin antibiotic combination with activity against vancomycin-resistant Enterococcus faecium (VREF), but emergence of resistance has been recently reported. We studied the activity of quinupristin-dalfopristin against two clinical strains of VREF (12311 and 12366) in an in vitro pharmacodynamic model with simulated endocardial vegetations (SEVs) to determine the potential for resistance selection and possible strategies for prevention. Baseline MICs/minimal bactericidal concentrations (μg/ml) for quinupristin-dalfopristin, quinupristin, dalfopristin, and doxycycline were 0.25/2, 64/&gt;512, 4/512, and 0.125/8 for VREF 12311 and 0.25/32, 128/&gt;512, 2/128, and 0.25/16 for VREF 12366, respectively. Quinupristin-dalfopristin regimens had significantly less activity against VREF 12366 than VREF 12311. An 8-μg/ml simulated continuous infusion was the only bactericidal regimen with time to 99.9% killing = 90 hours. The combination of quinupristin-dalfopristin every 8 h with doxycycline resulted in more killing compared to either drug alone. Quinupristin-dalfopristin-resistant mutants (MICs, 4 μg/ml; resistance proportion, ∼4 × 10 −4 ) emerged during the quinupristin-dalfopristin monotherapies for both VREF strains. Resistance was unstable in VREF 12311 and stable in VREF 12366. The 8-μg/ml continuous infusion or addition of doxycycline to quinupristin-dalfopristin prevented the emergence of resistance for both strains over the 96-h test period. These findings replicated the development of resistance reported in humans and emphasized bacterial factors (drug susceptibility, high inoculum, organism growth phase) and infectious conditions (penetration barriers) which could increase chances for clinical resistance. The combination of quinupristin-dalfopristin with doxycycline and the administration of quinupristin-dalfopristin as a high-dose continuous infusion warrant further study to determine their potential clinical utility.
Wireless internet gateways (WINGs) (2002)

Today's internetwork technology has been extremely successful in linking huge numbers of computers and users. However, to date, this technology has been oriented to computer interconnection in relatively stable operational environments, and thus cannot adequately support many of the emerging civilian and military uses that require a more adaptive and more easily deployed technology. In particular, multihop packet radio networks are ideal for establishing "instant communication infrastructures" in disaster areas resulting from flood, earthquake, hurricane, or fires, supporting US military doctrine for reliable, secure infrastructures for communication among all tiers down to the soldiers "on-the move" and extending the global communication infrastructure to the wireless, mobile environment. The Defense Advanced Research Projects Agency (DARPA) is sponsoring the development of wireless internet gateways (WINGs) as part of the DARPA Global Mobile (GloMo) Information Systems program. WINGs are wireless IP routers that enable the seamless marriage of distributed, dynamic, self-organizing, multihop wireless networks with the emerging multimedia Internet. This paper describes the WING architecture and novel communication protocols for channel access and routing, as well as the hardware and software development environment used to prototype and demonstrate wireless mobile internetworking.
A double blind, placebo-controlled, crossover therapy study with natural human IL-2 (nhuIL-2) in combination with regular intravenous gammaglobulin (IVIG) infusions in 10 patients with common variable immunodeficiency (CVID) (1997)

Ten CVID patients with defective IL-2 synthesis in vitro were treated with nhuIL-2 in a placebo-controlled, double blind, crossover therapy study during a period of 12 months. No severe side-effects of nhuIL-2 were recorded. Marginal serum nhuIL-2 levels were measurable in individual patients only during the therapy phase. Serum levels of soluble IL-2 receptors were unaffected by the therapy. nhuIL-2 and placebo groups did not differ significantly with respect to requirement of IVIG substitutions which were performed whenever serum IgG levels dropped below 5 g/l: a total of 53 IVIG infusions (corresponding to 17.6 g IgG/month per patient) was necessary during the placebo phase, and 48 infusions (16.4 g IgG/month per patient) during the nhuIL-2 treatment phase. Thus, nhuIL-2 therapy was ineffective in improving spontaneous IgG synthesis in vivo. Nevertheless, the group of patients receiving nhuIL-2 during the first 6 months of the study exhibited a significant reduction of severe infections (n = 25) during the following 6 months of placebo treatment (n = 7) (P<0.045). The infection score dropped in this group from 181 to 23 (P<0.015). Patients of the second group receiving first placebo and then nhuIL-2 did not experience a significant difference in number and score of infectious episodes: 25 infections were recorded during the first 6 months and 24 during the following 6 months. We suppose that nhuIL-2 therapy of CVID patients reduces susceptibility to severe infections, possibly via the induction of a specific antibody response, which is effective at the earliest 6 months after initiating nhuIL-2 therapy.
Cysteinesulfinate metabolism. altered partitioning between transamination and decarboxylation following administration of beta-methyleneaspartate. (1983)

L-Cysteinesulfinate, a quantitatively important catabolite of L-cysteine, is a substrate of both cysteinesulfinate decarboxylase and glutamate-oxaloacetate transaminase. The former enzyme initiates a pathway leading to taurine; the latter enzyme forms beta-sulfinyl-pyruvate, which spontaneously decomposes to pyruvate and SO2. In the present studies, the in vivo partitioning of cysteinesulfinate between these two pathways was evaluated by administering to mice L-[1-14C]cysteinesulfinate, which is metabolized to 14CO2 by both pathways, or L-[3-14C]cysteinesulfinate, which is converted to 14CO2 only if taurine is not formed. Within 6 h, respiratory 14CO2 accounted for 90% of the [1-14C]cysteinesulfinate injected, whereas only 18% of administered [3-14C]cysteinesulfinate was recovered as 14CO2. When the data are corrected for differences in the formation of 14CO2 from [1-14C]- and [3-14C]pyruvate and for a small formation of 14CO2 from radiolabeled hypotaurine, it is concluded that approximately 85% of administered cysteinesulfinate is decarboxylated to hypotaurine, whereas approximately 15% is transaminated. Of the hypotaurine formed, approximately 90% is oxidized to taurine. beta-Methylene-DL-aspartate, an irreversible inhibitor of glutamate-oxal-oacetate transaminase (Cooper, A.J.L., Fitzpatrick, S. M., Kaufman, C., and Dowd, P. (1982) J. Am. Chem. Soc. 104, 332-334) was given to mice with the expectation that conversion of cysteinesulfinate to hypotaurine would be increased. Surprisingly, the extent of cysteinesulfinate transamination increased about 3-fold. Additional studies indicate that beta-methyleneaspartate is a potent, irreversible inhibitor of purified rat liver cysteinesulfinate decarboxylase and that inactivation of the decarboxylase predominates over inactivation of the transaminase in vivo. Highly purified cysteinesulfinate decarboxylase is also shown to decarboxylate L-aspartate to beta-alanine and, very slowly, glutamate to gamma-aminobutyrate. The enzyme is not active toward alpha-methylcysteinesulfinate or alpha-methylaspartate; alpha-methyl-DL-[1-14C]cysteinesulfinate is not metabolized by the mouse.
Facultad de Medicina, en el Campus de la Salud de la Universidad de Granada (2017)

El conjunto edificado de la Facultad de Medicina de la ciudad de Granada participa en su estrategia del esquema previsto por la ordenacion general del propio campus, en el que horizontalidad y verticalidad permiten referenciar sus diferentes usos en la escala urbana.

La Facultad de Medicina ocupa tres de las barras situadas al oeste del edificio central. Cada una de estas se compone a su vez de una banda horizontal de dos plantas mas semisotano para usos docentes y generales, y una torre de nueve plantas para departamentos. Las tres piezas se conectan entre si mediante pasarelas. El conjunto edificado sigue el esquema asignado en la ordenacion general del campus, que pretende una marcada horizontalidad en las barras destinadas a la docencia, dispuestas en torno al edificio central, y un caracter referencial a escala urbana en las torres de los departamentos que se asoman a los bordes del campus. 

El esquema general de todos los edificios que componen el campus garantiza la variedad dentro de la unidad reconocible y con una identidad lo bastante clara como para destacar en un entorno dificil. Con esta composicion se combina el concepto de campus como espacio comunitario de escala tranquila, con la necesidad de consistencia e identidad que requiere un lugar como este, situado en el limite difuso entre ciudad y zona metropolitana.

En la fachada oeste de la facultad, una plaza ajardinada construye un espacio previo de acceso a traves de una gran marquesina y proporciona al edificio un ambito propio que posibilita un alejamiento del trafico, lo que permite una vision amplia del conjunto.

La conexion con el resto del conjunto universitario se realiza a traves de una red de caminos peatonales. Las dos calles interiores que configuran los tres volumenes del edificio se utilizan no solo como espacios contemplativos o de iluminacion sino tambien como parte integral de la red de espacios libres. Estos espacios ajardinados estan abiertos al resto del campus y aseguran la permeabilidad de todo el conjunto. 

Los distintos usos se organizan distribuyendo en la barra central los mas publicos o representativos (salon de actos, cafeteria, asociaciones, etc.) y en las laterales los docentes (aulas y laboratorios).

Las tres torres que albergan los departamentos, de 11 pisos de altura, tienen un funcionamiento analogo, con un nucleo de comunicaciones formado por escalera, ascensores, huecos de instalaciones y cuartos humedos, y unos espacios libres que se ocupan por bandas de despachos dejando el resto disponible para las necesidades concretas de cada departamento. El espacio de circulacion adopta una forma organica, configurando espacios mas amplios de estancia, descanso, reunion, etc. y definiendo asi unos patios curvos de formas libres que se ajardinan con arboles.
Gene Expression Profiles in Normal and Cancer Cells (1997)

As a step toward understanding the complex differences between normal and cancer cells in humans, gene expression patterns were examined in gastrointestinal tumors. More than 300,000 transcripts derived from at least 45,000 different genes were analyzed. Although extensive similarity was noted between the expression profiles, more than 500 transcripts that were expressed at significantly different levels in normal and neoplastic cells were identified. These data provide insight into the extent of expression differences underlying malignancy and reveal genes that may prove useful as diagnostic or prognostic markers.
Expression of Biologically Active Fusion Genes Encoding the Common α Subunit and the Follicle-stimulating Hormone β Subunit (1996)

The gonadotropin/thyrotropin hormone family is characterized by a heterodimeric structure composed of a common alpha subunit noncovalently linked to a hormone-specific beta subunit. The conformation of the heterodimer is essential for controlling secretion, hormone-specific post-translational modifications, and signal transduction. Structure-function studies of follicle-stimulating hormone (FSH) and the other glycoprotein hormones are often hampered by mutagenesis-induced defects in subunit combination. Thus, the ability to overcome the limitation of subunit assembly would expand the range of structure-activity relationships that can be performed on these hormones. Here we converted the FSH heterodimer to a single chain by genetically fusing the carboxyl end of the FSH beta subunit to the amino end of the alpha subunit in the presence or absence of a linker sequence. In the absence of the CTP linker, the secretion rate was decreased over 3-fold. Unexpectedly, however, receptor binding/signal transduction was unaffected by the absence of the linker. These data show that the single-chain FSH was secreted efficiently and is biologically active and that the conformation determinants required for secretion and biologic activity are not the same.
Next generation sequencing (NGS) in wild type GISTs. (2017)

e22507 Background: The majority driven mutation of GIST are located in KIT and PDGFRA. A proportion of the remaining 10% of GIST without KIT or PDGFRA mutations called wild type GIST (wt-GIST). It is poor response to imatinib, sunitinib or regorafenib in these wt-GIST patients. It is lack of precise drug target of wt-GIST. We analyzed next generation exome sequencing (NGS) results in wt-GISTs. Methods: Whole exome sequencing was performed on freezing tumor tissue and peripheral blood DNA with 100X sequencing depth. The low frequency somatic mutation was confirmed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and Sanger sequencing. This study was approved by the ethical committee of Sun Yat-Sen University Cancer Center. Results: A total of 11 wt-GIST samples were analyzed. ROS1 (ROS Proto-Oncogene 1, Receptor Tyrosine Kinase) was mutated in one CD117 negative patients with 5.3% mutation frequency of c. 364G &gt; A (p. A122T). Sanger sequencing couldn’t found the ROS1 mutated in tumor tissue. But this low frequency somatic mutation was verified by MALDI-TOF. Conclusions: This is the first report showed a new ROS1 somatic mutation in wt-GIST. Our results indicated that ROS1 could be a new possible driven mutation in wt-GIST. ROS1 rearrangement have been described in a subset of non-small-cell lung cancers (NSCLC). Crizotinib shows a potent curative effect in ROS1 rearrangement NSCLC. Therefore, crizotinib might be an appropriate drug to GIST patients with mutated ROS1.
Targeting CpG Oligonucleotides to the Lymph Node by Nanoparticles Elicits Efficient Antitumoral Immunity (2008)

Viral nucleic acids are recognized by specific pattern-recognition receptors of the Toll-like and RIG-I-like receptor families. Synthetic DNA and RNA oligonucleotides can activate the immune system through these receptors and potentiate Ab and CD8 cytotoxic responses to Ags. Systemic application of immunostimulatory oligonucleotides however also results in a generalized, non-Ag-specific stimulation of the immune system. In this study, we have dissociated the induction of an Ag-specific response from the systemic immune activation generally associated with immunostimulatory oligonucleotides. Delivery of CpG oligodeoxynucleotides that bind TLR9 by cationized gelatin-based nanoparticles potentiates the in vivo generation of an Ag-specific cytotoxic T cell and Ab response. Furthermore, immunization with CpG-loaded nanoparticles induces a protective antitumoral response in a murine model of melanoma. The systemic release of proinflammatory cytokines and widespread immunostimulation associated with free CpG is however completely abolished. In addition, we show that gelatin nanoparticle formulation prevents the destruction of lymphoid follicles mediated by CpG. Nanoparticle-delivered CpG, in contrast to free CpG, are selectively targeted to APCs in the lymph nodes where they mediate local immune stimulation. We describe a novel strategy to target immunostimulatory oligonucleotides to the initiation site of the immune response while at the same time protecting from an indiscriminate and generalized activation of the immune system.
Expression of nonphagocytic NADPH oxidase system in the ocular lens. (2004)

These data demonstrate the presence of a functional nonphagocytic NADPH oxidase system in lens that is predominantly localized to the lens epithelium. Several growth factors appear to stimulate the activity of lens NADPH oxidase, resulting in increased production of ROS in lens epithelial cells, indicating that redox signaling may have an important role in growth factor effects on lens growth and development.
Encyclopedia of bioprocess technology: fermentation, biocatalysis, and bioseparation (1999)

The five--volume set of the Encyclopedia of Bioprocess Technology presents the applications and established theories in biotechnology--focusing on industrial applications of fermentation, biocatalysis and bioseparation. It is an essential resource for anyone working in industrial biotechnology, biochemistry, genetics and microbiology laboratories, pharmaceutical firms, regulatory agencies and chemical and environmental engineering companies.
Distinct mechanisms of action of anti‐CD154 in early versus late treatment of murine lupus nephritis (2003)

Amelioration of murine lupus by anti-CD154 therapy is mediated by distinct mechanisms in early versus late intervention. We postulate that anti-CD154 therapy prevents autoantibody production and renal immune complex deposition in the early, induction phase and limits secondary tissue damage in situ in the late, effector phase. These data demonstrate that CD40-CD154 interactions are critical for the maintenance of autoimmunity and suggest a potential role for anti-CD154 as a therapeutic agent in established human lupus.
By-product formation during exposure of respiring Saccharomyces cerevisiae cultures to excess glucose is not caused by a limited capacity of pyruvate carboxylase (1999)

Upon exposure to excess glucose, respiring cultures of Saccharomyces cerevisiae produce substantial amounts of ethanol and acetate. A possible role of a limited anaplerotic capacity in this process was investigated by overexpressing pyruvate carboxylase and by replacing it with a heterologous enzyme (Escherichia coli phosphoenolpyruvate carboxylase). Compared to the wild-type, neither the pyruvate carboxylase (Pyc)-overexpressing nor the transgenic strain exhibited reduced by-product formation after glucose pulses to aerobic glucose-limited chemostat cultures. An increased intracellular malate concentration was observed in the two engineered strains. It is concluded that by-product formation in S. cerevisiae is not caused by a limited anaplerotic capacity.
Oral health of Chinese people with systemic sclerosis (2010)

The aim was to study oral health status, salivary function, and oral features of Chinese people with Systemic Sclerosis (SSc). Chinese people with SSc attending a university specialist clinic were invited for a questionnaire survey and a clinical examination. Ethics approval was sought (UW 08-305). Gender- and age-matched individuals without SSc who attended a university dental hospital were recruited for comparison. Forty-two SSc patients with a mean age of 54.0 ± 12.2 were examined. This study found no Chinese people with systemic sclerosis were periodontally healthy and many (76%) had periodontal pockets despite most of them (93%) practiced daily tooth-brushing. They all had caries experience (DMFT = 10.5) and many (65%) had untreated decay. Mucosal telangiectasia was a common oral feature (80%). They had lower resting salivary flow rates (0.18 ± 0.17 ml/min vs. 0.31 ± 0.21 ml/min; p = 0.003) and pH values (6.90 ± 0.40 vs. 7.28 ± 0.31; p < 0.001) and reduced maximal mouth opening (40.1 ± 6.5 mm vs. 43.6 ± 7.0 mm) than people without SSc.