gebhart/patrepeval huggingface dataset:
Selection and Characterization of Sethoxydim- Tolerant Maize Tissue Cultures (1990)

;Black Mexican Sweet' (BMS) maize (Zea mays L.) tissue cultures were selected for tolerance to sethoxydim. Sethoxydim, a cyclohexanedione, and haloxyfop, an aryloxyphenoxypropionate, exert herbicidal activity on most monocots including maize by inhibiting acetyl-coenzyme A carboxylase (ACCase). Selected line B10S grew on medium containing 10 micromolar sethoxydim. Lines B50S and B100S were subsequent selections from B10S that grew on medium containing 50 and 100 micromolar sethoxydim, respectively. Growth rates of BMS, B10S, B50S, and B100S were similar in the absence of herbicide. Herbicide concentrations reducing growth by 50% were 0.6, 4.5, 35, and 26 micromolar sethoxydim and 0.06, 0.5, 5.4, and 1.8 micromolar haloxyfop for BMS, B10S, B50S, and B100S, respectively. Sethoxydim and haloxyfop concentrations that inhibited ACCase by 50% were similar for BMS, B10S, B50S, and B100S. However, ACCase activities were 6.01, 10.7, 16.1, and 11.4 nmol HCO(3) (-) incorporated per milligram of protein per minute in extracts of BMS, B10S, B50S, and B100S, respectively, suggesting that increased wild-type ACCase activity conferred herbicide tolerance. Incorporation of [(14)C]acetate into the nonpolar lipid fraction was higher for B50S than for BMS in the absence of sethoxydim providing further evidence for an increase in ACCase activity in the selected line. In the presence of 5 micromolar sethoxydim, [(14)C]acetate incorporation by B50S was similar to that for untreated BMS. The levels of a biotin-containing polypeptide (about 220,000 molecular weight), presumably the ACCase subunit, were increased in the tissue cultures that exhibited elevated ACCase activity indicating overproduction of the ACCase enzyme.
The role of disrupted iron homeostasis in the development and progression of arthropathy (2022)

Arthropathy or joint disease leads to significant pain and disability irrespective of etiology. Clinical and experimental evidence point to the presence of considerable links between arthropathy and iron overload. Previous work has suggested that iron accumulation in the joints is often associated with increased oxidative stress, disrupted matrix metabolism, and cartilage degeneration. However, key issues regarding the role of iron overload in the pathogenesis of arthropathy remain ambiguous. For example, significant gaps in our knowledge of the primary cellular targets of iron overload-induced damage and the exact molecular mechanism through which disrupted iron homeostasis leads to joint damage still exist. The exact signaling pathway that links iron metabolism and cellular damage in arthropathy also remains largely unmapped. In this review, we focus on the relationship between iron overload and arthropathy with special emphasis on the adversarial relationship between iron that accumulates in the joints over time and cartilage homeostasis. A better understanding of the mechanisms and pathways underlying iron-induced cartilage degeneration may help in defining new prognostic markers and therapeutic targets in arthropathy.
Erratum: “Spin filtering in a magnetic-electric barrier structure” [Appl. Phys. Lett. 78, 2184 (2001)] (2001)

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Psychoproteomic Analysis of Rat Cortex Following Acute Methamphetamine Exposure (2008)

Methamphetamine (METH) is recognized as one of the most abused psychostimulants in the United States. METH is an illicit drug that is known to exert neurotoxic effects on both dopaminergic and serotonergic neural systems both in vivo and in vitro. Our laboratory and others have been studying the biochemical mechanisms underlying METH-induced neurotoxicity. Here, we applied a novel psychoproteomic approach to evaluate METH-induced neurotoxicity following acute METH administration (4x10 mg/kg, ip injections every 1 h). Samples of cortical tissue collected 24 h post METH treatment were pooled, processed and analyzed via a selective psychoproteomic platform. Protein separation was performed using our previously established offline tandem cation-anion exchange chromatography-SDS-1D-PAGE platform (CAX-PAGE). Gel bands exhibiting 2 or more fold changes were extracted, trypsinized and subjected to reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) analyses for protein identification. Differential changes of the selected proteins were further confirmed by quantitative immunoblotting. We identified 82 differentially expressed proteins, 40 of which were downregulated and 42 of which were upregulated following acute METH treatment. Proteins that decreased in abundance included collapsin response mediator protein-2 (CRMP-2), superoxide dismutase 1 (SOD 1), phosphatidylethanolamine-binding protein-1 (PEBP-1) and mitogen activated kinase kinase-1 (MKK-1). Proteins that increased in abundance included authophagy-linked microtubule-associated protein light chain 3 (LC3), synapsin-1, and Parkinsonism linked ubiquitin carboxy-terminal hydroxylase-L1 (UCH-L1). Lastly, we used these differentially expressed protein subsets to construct a "psychoproteomic" spectrum map in an effort to uncover potential protein interactions relevant to acute METH neurotoxicity.
Studies on the cytophilic properties of human beta2 microglobulin. (1975)

Human beta2 microglobulin is cytophilic for mouse, rat, and to a lesser extent guinea pig lymphocytes and polymorphonuclear leukocytes but not for human, rabbit, chicken, frog, and turtle cells, nor for any mature erythrocytes tested. Mouse splenic lymphocytes bind 5- 7-fold more beta2 microblobulin than thymocytes although hydrocortisone-resistant thymocytes resemble spleen cells in this regard. The beta2 microglobulin binding by B and T cells in mouse spleen is similar. The structure responsible for beta2 microglobulin binding to the surface of cells is distinct from the Fc receptor specific for aggregated IgG.
Deamination of bridgehead amines via the nitroso- and nitroamide approach (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDeamination of bridgehead amines via the nitroso- and nitroamide approachEmil H. White, Richard H. McGirk, Carl A. Aufdermarsh, H. P. Tiwari, and Michael J. ToddCite this: J. Am. Chem. Soc. 1973, 95, 24, 8107–8113Publication Date (Print):November 1, 1973Publication History Published online1 May 2002Published inissue 1 November 1973https://pubs.acs.org/doi/10.1021/ja00805a026https://doi.org/10.1021/ja00805a026research-articleACS PublicationsRequest reuse permissionsArticle Views160Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
GM-CSF up-regulates the expression of CCL2 by T lymphocytes in mammary tumor-bearing mice (2007)

MCP-1/CCL2 (monocyte chemoattractant protein-1/CC chemokine ligand 2) is a beta or CC chemokine that is expressed by a variety of cell types, including fibroblasts, endothelial, smooth muscle, and glial cells. In addition, cells involved in immunity, such as monocytes/macrophages, neutrophils, and eosinophils have also been shown to express this chemoattractant. Using a murine model of the D1-DMBA-3 mammary adenocarcinoma, we demonstrated the unique production of CCL2 by splenic T lymphocytes from tumor-bearing animals. Because this tumor produces GM-CSF, and this factor is also up-regulated in the B lymphocytes of tumor-bearing mice, we looked at the ability of GM-CSF to induce CCL2 production by T cells. Treatment of normal and tumor bearers' T cells with GM-CSF resulted in an increased secretion of this chemokine. This up-regulation was seen with or without stimulation by Concanavalin A, although these treatments were additive in their effects. The induction of CCL2 was studied at the molecular level by analyzing the effect(s) of a variety of physiological and pharmacological agents on cultured T cells. These results suggest that the tumor-derived factor GM-CSF activates various signaling pathways within splenic T cells to up-regulate CCL2 expression.
Prostate‐specific antigen recurrence and mortality after conventional dose radiation therapy in select men with low‐risk prostate cancer (2006)

In men age >70 years with low-risk prostate cancer and pretreatment PSA velocity < or =1.0 ng/mL/year, prostate cancer death was not observed despite a modest PSA recurrence rate.
Depth-Sensing Video Cameras for 3D Tangible Tabletop Interaction (2007)

Recently developed depth-sensing video camera technologies provide precise per-pixel range data in addition to color video. Such cameras will find application in robotics and vision-based human computer interaction scenarios such as games and gesture input systems. We present an interactive tabletop system which uses a depth-sensing camera to build a height map of the objects on the table surface. This height map is used in a driving simulation game that allows players to drive a virtual car over real objects placed on the table. Players can use folded bits of paper, for example, to lay out a course of ramps and other obstacles. A projector displays the position of the car on the surface, such that when the car is driven over a ramp, for example, it jumps appropriately. A second display shows a synthetic graphical view of the entire surface, or a traditional arcade view from behind the car. Micromotorcross is a fun initial investigation into the applicability of depth-sensing cameras to tabletop interfaces. We present details on its implementation, and speculate on how this technology will enable new tabletop interactions.
Nonsupervised numerical component extraction from pyrolysis mass spectra of complex mixtures (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNonsupervised numerical component extraction from pyrolysis mass spectra of complex mixturesWillem. Windig and Henk L. C. MeuzelaarCite this: Anal. Chem. 1984, 56, 13, 2297–2303Publication Date (Print):November 1, 1984Publication History Published online1 May 2002Published inissue 1 November 1984https://pubs.acs.org/doi/10.1021/ac00277a009https://doi.org/10.1021/ac00277a009research-articleACS PublicationsRequest reuse permissionsArticle Views68Altmetric-Citations72LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Applications of Continuous and Steady-State Methods to Root Biology. (1990)

1. A Multichannel System for Steady-State and Continuous Measurements of Gas Exchanges from Legume Roots and Nodules.- 1. Introduction.- 2. Overview of the Gas Exchange System.- 3. Application of the Gas Exchange System to Studies of Legume Physiology.- 4. Conclusions.- 2. Quantification of Diffusion Characteristics in Spherical Nodules: A Comparison of Methods.- 1. Introduction.- 2. Homogeneous Model of Nodule Diffusion.- 3. Diffusion Barrier Models.- 4. Comparisons Among Diffusion Barrier Models.- 5. Conclusions.- 6. Appendix. Sensitivity of Transient Analysis to Changes in Vmx.- 3. Simple Apparatus for Growth of Nodulated Plants and for Continuous Nitrogenase Assay Under Defined Gas Phase.- 1. Introduction.- 2. Apparatus - Plant Growth in Defined O2 Levels.- 3. Apparatus - Assays in Open, Flow-Through Systems.- 4. Continuous Measurements of Nitrogenase Activity in the Study of Environmental Responses of Frankia and Other Bacteria.- 1. Introduction.- 2. Materials and Methods.- 3. Results.- 4. Discussion.- 5. Limitations and Errors in Gas Exchange Measurements with Legume Nodules.- 1. Introduction.- 2. Sources of Error.- 3. Avoidance of the Acetylene-Induced Decline.- 4. Conclusions.- 6. Principles and Approaches in Modeling Steady-State Gas Diffusion in Legume Nodules.- 1. Introduction.- 2. General Diffusion Models.- 3. A Model of Gas Diffusion in Legume Nodules.- 4. Other Models of Gas Diffusion.- 7. Modeling Gas Exchange by Actinorhizal Root Nodules Using Network Simulation Analysis.- 1. Introduction.- 2. Model Construction.- 3. Simulation Model Output.- 4. Conclusions.- 8. Continuous and Steady-State Nutrient Absorption by Intact Plants.- 1. Introduction.- 2. Nutrient Flow System.- 3. Ion-Selective Electrodes.- 4. Measurement Artifacts.- 5. Validity of Continuous and Steady-State Measurements.- 9. Steady-State Control and Investigation of Root System Morphology.- 1. Introduction.- 2. Overview.- 3. Environmental Factors which Affect Roots.- 4. Aeroponics.- 5. Conclusion.- 10. Measurement of Carbon Cost in Ectomycorrhizae.- 1. Introduction.- 2. Carbon Cost as Loss of Potential Dry Matter.- 3. Carbon Flow from Shoot to Root.- 4. Carbon Flow from Root to Fungus.- 5. Modeling Carbon Flow in Ectomycorrhizae.- 6. Conclusions.- 11. Approaches to Measuring Soil Nitrogen Transformations Under Continuous or Steady-State Conditions.- 1. Introduction.- 2. The Soil Environment.- 3. Soil Nitrogen Transformations.- 4. Continuous and Steady-State Terminology.- 5. Laboratory Methods.- 6. Field Methods.- 7. Isotope Dilution.- 8. Summary.- 12. Methodological Considerations in Measuring Biomass, Production, Respiration and Nutrient Resorption for Tree Roots in Natural Ecosystems.- 1. Introduction.- 2. Problems Associated with Field Root Studies.- 3. Root Biomass and Production.- 4. Root Respiration.- 5. Root Resorption of Nutrients.- 6. Conclusions.- Authors' Index.
Ionic and Energetic Changes at Competence in the Naturally Transformable Bacterium Streptococcus pneumoniae (1989)

Addition of competence factor extracts to trigger competence in a culture of Streptococcus pneumoniae induced an increase in the intracellular pH and the Na+ content of the bacteria without any change in the K+ pool or in the membrane potential. These ionic shifts were concomitant with a stimulation of glycolysis that resulted in an enhanced ATP pool. Thus, in transforming conditions, at extracellular pH 7.8, competent bacteria presented a particularly high energetic state resulting from an increase in delta pH and in the ATP pool, associated with an enhanced Na+ content. These features are discussed in the context of homeostasis regulation in response to an environmental stimulus.
Cellular Uptake, Distribution, and Stability of 10-23 Deoxyribozymes (2002)

The cellular uptake, intracellular distribution, and stability of 33-mer deoxyribozyme oligonucleotides (DNAzymes) were examined in several cell lines. PAGE analysis revealed that there was a weak association between the DNAzyme and DOTAP or Superfect transfection reagents at charge ratios that were minimally toxic to cultured cells. Cellular uptake was analyzed by cell fractionation of radiolabeled DNAzyme, by FACS, and by fluorescent microscopic analysis of FITC-labeled and TAMRA-labeled DNAzyme. Altering DNAzyme size and chemistry did not significantly affect uptake into cells. Inspection of paraformaldehyde-fixed cells by fluorescence microscopy revealed that DNAzyme was distributed primarily in punctate structures surrounding the nucleus and that substantial delivery to the nucleus was not observed up to 24 hours after initiation of transfection. Incubation in human serum or plasma demonstrated that a 3'-inversion modification greatly increased DNAzyme stability (t(1/2) approximately 22 hours) in comparison to the unmodified form (t(1/2) approximately 70 minute). The 3'-inversion-modified DNAzymes remained stable during cellular uptake, and catalytically active oligonucleotide could be extracted from the cells 24 hours posttransfection. In smooth muscle cell proliferation assay, the modified DNAzyme targeting the c-myc gene showed a much stronger inhibitory effect than did the unmodified version. The present study demonstrates that DNAzymes with a 3'-inversion are readily delivered into cultured cells and are functionally stable for several hours in serum and within cells.
Near-Infrared Absorbance of Single-Walled Carbon Nanotubes Dispersed in Dimethylformamide (2003)

Absorption spectra of single-walled carbon nanotubes (SWNT) dispersed in dimethylformamide (DMF) have been recorded in the visible to near-infrared range. Besides broad absorption bands, the spectra show a sharp peak at 5187 cm-1, which has been attributed in the literature to the absorption of semiconducting nanotubes with a band gap of 0.64 eV [Ausman et al. J. Phys. Chem. B 2000, 104, 8911]. On the basis of absorbance measurements for samples that show the sharp absorbance peak but do not contain SWNTs, we conclude that the origin of the peak at 5187 cm-1 is not due to semiconducting nanotubes but due to water present in the SWNT/DMF suspension. A comparative study of D2O in DMF reproduces a similar peak that is isotope-shifted into the near-infrared.
Real-time application of neural networks for sensor-based control of robots with vision (1989)

A practical neural network-based learning control system is described that is applicable to complex robotic systems involving multiple feedback sensors and multiple command variables. In the controller, one network is used to learn to reproduce the nonlinear relationship between the sensor outputs and the system command variables over particular regions of the system state space. The learned information is used to predict the command signals required to produce desired changes in the sensor outputs. A second network is used to learn to reproduce the nonlinear relationship between the system command variables and the changes in the video sensor outputs. The learned information from this network is then used to predict the next set of video parameters, effectively compensating for the image processing delays. The results of learning experiments using a General Electric P-5 manipulator are presented. These experiments involved control of the position and orientation of an object in the field of view of a video camera mounted on the end of the robot arm, using moving objects with arbitrary orientation relative to the robot. No a priori knowledge of the robot kinematics or of the object speed of orientation relative to the robot was assumed. Image parameter uncertainty and control system tracking error in the video image were found to converge to low values within a few trials.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Prevascularization of a Fibrin-Based Tissue Construct Accelerates the Formation of Functional Anastomosis with Host Vasculature (2008)

One critical obstacle facing tissue engineering is the formation of functional vascular networks that can support tissue survival in vivo. We hypothesized that prevascularizing a tissue construct with networks of well-formed capillaries would accelerate functional anastomosis with the host upon implantation. Fibrin-based tissues were prevascularized with capillary networks by coculturing human umbilical vein endothelial cells (HUVECs) and fibroblasts in fibrin gels for 1 week. The prevascularized tissue and nonprevascularized controls were implanted subcutaneously onto the dorsal surface of immune-deficient mice and retrieved at days 3, 5, 7 and 14. HUVEC-lined vessels containing red blood cells were evident in the prevascularized tissue by day 5, significantly earlier than nonprevascularized tissues (14 days). Analysis of the HUVEC-lined vessels demonstrated that the number and area of perfused lumens in the prevascularized tissue were significantly larger compared to controls. In addition, collagen deposition and a larger number of proliferating cells were evident in the prevascularized tissue at day 14. Our results demonstrate that prevascularizing a fibrin-based tissue with well-formed capillaries accelerates anastomosis with the host vasculature, and promotes cellular activity consistent with tissue remodeling. Our prevascularization strategy may be useful to design large three-dimensional engineered tissues.
Intradermal Implantation of Bovine Collagen (1984)

A special collagen implant (Zyderm) (ZCI) is a biomaterial used for the correction of dermal contour deformities. Although infrequent, reactions believed to be of an immunologic nature have followed treatment. To investigate whether humoral immunity is associated with these reactions, we studied 40 patients for the presence of anti-ZCI antibodies using an enzyme-linked immunosorbent assay. Ten patients who had had adverse treatment reactions uniformly exhibited circulating anti-ZCI antibodies. Twenty-nine of 30 control patients, including nine who had received ZCI without adverse reaction, failed to produce the high levels of antibodies found in all clinical reactors. This difference was statistically significant. Additional investigations confirmed that the antibodies studied were directed primarily against the bovine collagen component of the ZCI. These data suggest that the humoral immune system is capable of recognizing ZCI and that the presence of antibodies to the ZCI correlates with the occurrence of adverse reactions.
Toxicological Aspects of Vanadyl Sulphate on Diabetic Rats: Effects on Vanadium Levels and Pancreatic B‐Cell Morphology (1990)

This study explored some toxicological aspects of vanadyl sulphate (VOSO4) treatment of rats made diabetic with a single intravenous injection of streptozotocin (60 mg/kg). Administered in drinking water (0.25, 0.5, 0.75 or 1 mg of VOSO4, 5H2O ml) VOSO4 treatment partially or totally corrected some of the alterations associated with the diabetic state (hyperglycaemia, polydipsia, polyphagia, high cholesterol and triglycerides levels) and did not produce any changes in various plasma or blood cell parameters which were not previously altered by diabetes. Measurement of vanadium levels indicated that tissues accumulated vanadium in the following order of concentrations: bone greater than kidney greater than spleen greater than liver greater than lung greater than or equal to muscle greater than blood. Histopathological studies did not reveal any difference in liver, stomach, ileum, spleen, heart and lung from control, non-treated diabetic or VOSO4-treated diabetic animals. Kidney of all non-treated diabetic animals showed an epithelial cellular swelling of distal tubules while only 2 of 6 VOSO4-treated diabetic animals showed this alteration. Cellular degeneration of pancreas B-cells was less marked in VOSO4-treated that in non-treated diabetic animals. The study indicates that VOSO4 may be a potential antidiabetic agent.