gebhart/patrepeval huggingface dataset:
Campylobacter jejuni infection in the ferret: An animal model of human campylobacteriosis (1987)

Campylobacter infection in weanling ferrets (Mustela putorius furo) was studied as an animal model for enteric campylobacteriosis in persons. The screening of fecal cultures on selective campylobacter media showed that Campylobacter jejuni/coli was not present in the normal enteric flora. Intragastric feeding of a mixture of cat feed and 2.5 X 10(8) C jejuni isolated from ferrets with naturally occurring proliferative colitis was accomplished. All ferrets (n = 8) became infected on 3 days after they were inoculated, and at 5 to 7 days, they had bile-tinged, liquid feces with excessive mucus and blood. Ferrets gradually recovered from the diarrhea, and feces were normal 10 to 14 days after inoculation was done. Feces contained C jejuni at 14, 23, 28, 39, 46, 60, 91, 101, 109 and 144 days. In the second experiment, weanling ferrets initially were treated with 10% sodium bicarbonate, and 1 X 10(10) C jejuni organisms were administered in the cat feed. Diarrhea with fecal leukocytes and occult blood with occasional mucus appeared in almost all of the 21 ferrets from days 4 through 7. Campylobacter jejuni was isolated from the blood of 11 ferrets between 3 hours and 14 days after they were inoculated. Campylobacter jejuni bactericidal antibodies were present in serum samples at 14 days, with titers of 1:16 to 1:32. Intestinal lesions including cellular infiltration with mononuclear and polymorphonuclear leukocytes were in the lamina propria of the pyloric mucosa and small intestine of infected and control ferrets. The colon of 3 infected ferrets had small focal infiltrates of neutrophils on the lamina propria; one ferret had perivascular cuffing.(ABSTRACT TRUNCATED AT 250 WORDS)
PK11195, an isoquinoline carboxamide ligand of the mitochondrial benzodiazepine receptor, increased drug uptake and facilitated drug-induced apoptosis in human multidrug-resistant leukemia cells in vitro. (2002)

The isoquinoline peripheral benzodiazepine receptor ligand PK11195 increased drug (daunomycin)- and fluorochrome (calcein-AM) uptake and induced apoptosis detected by flow cytometry (FCM) technique, DNA electrophoretic analysis and poly(ADP-ribose) polymerase (PARP) cleavage in human multidrug-resistant myeloid leukemia (BL-60/VCR) and ovarian carcinoma (A2780/ADR) cells in vitro. The position of PK11195 with respect to drug-resistance modulator (DRM) efficiency, compared to the reference DRMs with the aid of FCM technique, was as follows: PSC833 > verapamil > PK11195 > vincristine. Our data show up to now not indicated observation that PK11195 possesses multidrug resistance modulating activity.
The Incorporation and Metabolism of Glucose by Anabaena variabilis (1968)

SUMMARY: The incorporation and metabolism of glucose by the blue-green alga Anabaena variabilis is described. Experiments with [14C]glucose indicated that this compound contributed up to 46 % of the total dry wt of organism. Respiratory studies with [I-14C] and [6-14C] showed that the pentose phosphate pathway was the major route of glucose dissimilation. No evidence of alteration in enzyme concentration after growth in the presence of glucose was found in the ten examples of glycolytic and pentose phosphate pathway enzymes examined. Enzymes from both pathways were affected by the end-products. These results are discussed in relation to autotrophic metabolism in blue-green algae.
Hepatitis C: progress and problems (1994)

The hepatitis C virus (HCV), a single-stranded RNA virus, is the major cause of posttransfusion hepatitis. HCV isolates differ in nucleotide and amino acid sequences. Nucleotide changes are concentrated in hypervariable regions and may be related to immune selection. In most immunocompetent persons, HCV infection is diagnosed serologically, using antigens from conserved regions. Amplification of RNA may be necessary to detect infection in immunosuppressed patients. Transmission by known parenteral routes is frequent; other means of spread are less common and may represent inapparent, percutaneous dissemination. Infection can lead to classical acute hepatitis, but most infected persons have no history of acute disease. Once infected, most individuals apparently remain carriers of the virus, with varying degrees of hepatocyte damage and fibrosis ensuing. Chronic hepatitis may lead to cirrhosis and hepatocellular carcinoma. However, disease progression varies widely, from less than 2 years to cirrhosis in some patients to more than 30 years with only chronic hepatitis in others. Determinants important in deciding outcome are unknown. Alpha interferon, which results in sustained remission in selected patients, is the only available therapy. Long-term benefits from such therapy have not been demonstrated. Prevention of HCV infection by vaccination is likely to be challenging if ongoing viral mutation results in escape from neutralization and clearance.
Lipoxygenase contributes to the oxidation of lipids in human atherosclerotic plaques. (1995)

Oxidized LDL is present in human atherosclerotic lesions, but the mechanisms responsible for oxidation in vivo have not been definitively demonstrated. Circumstantial evidence has implicated the enzyme 15-lipoxygenase as a contributor to the formation of oxidized lipids in this disease. To assess whether oxidized lipids are indeed formed by the action of 15-lipoxygenase on polyunsaturated fatty acids (PUFAs) in vivo, we have used a sensitive and specific method (chiral phase HPLC) to analyze the lipid oxidation products present in human atherosclerotic lesions. Human 15-lipoxygenase is an omega-6 lipoxygenase that has previously been shown to oxidize esterified PUFA in a stereospecific manner, forming predominantly cholesteryl hydroperoxy-octadecadienoate (13(S)-HPODE) from cholesteryl linoleate substrate in LDL. This property allows its activity to be distinguished from nonenzymatic oxidation, which results in the formation of equal quantities of the S and R stereoisomers of the same oxidation product. A total of 80 specimens of human atherosclerotic plaque were analyzed. Esterified, oxidized linoleate was purified from human atherosclerotic lesions and from LDL oxidized by copper, and the chirality of these oxidation products was compared. There was significantly greater stereospecificity of oxidation in the oxidized linoleate from human atherosclerotic lesions. Even greater stereospecificity was detected in the HPODE derived from cholesteryl ester, purified from human lesions. Cholesteryl HPODE is the primary oxidation product from cholesteryl linoleate, the major esterified PUFA that accumulates in atherosclerotic vessels. Cholesteryl HPODE and its reduced form, cholesteryl hydroxy-octadecadienoate, were detected in all lesions analyzed. Neither the stereospecificity of oxidation nor the percentage of available substrate oxidized to primary oxidation products was correlated with the stage of disease of the lesions examined. We conclude that 15-lipoxygenase contributes to the formation of oxidized lipids in human atherosclerotic lesions.
Construction of 3-oxyindoles via hypervalent iodine mediated tandem cyclization–acetoxylation of o-acyl anilines (2010)

An efficient tandem cyclization-acetoxylation of o-acyl anilines mediated by the combination of iodobenzene diacetate with tetrabutylammonium iodide provides a new convenient and useful route to 2-acetoxy indolin-3-ones, which are ready to be converted into other 2-substituted 3-oxyindole derivatives.
A Proposed Standard Fog Collector for Use in High-Elevation Regions (1994)

The collection of fog droplets by vegetation is an important wet deposition process. It can, in fact, dominate the chemical and hydrological input to certain high elevation watersheds. However, measurements of fog deposition are rarely made and, where they do exist, comparisons of deposition rates in different locations have been hampered by the use of innumerable types of collection devices. A simple, inexpensive, 1-m2 fog collector that can produce measurements of the deposition of fog water to a vertical surface is described here. The collector has been used successfully in five countries to investigate the variation of fog deposition in complex terrain and to estimate the deposition to trees and to much larger fog collectors. It is proposed that it be employed widely as a standard to quantify the importance of fog deposition to forested high elevation areas and to measure the potential collection rates in denuded or desert mountain ranges. The standard fog collector costs about the same as a rain gauge ($100 U.S.) to construct and can be used with a variety of recording devices. It is a flat panel made of a durable polypropylene mesh and mounted with its base 2 m above ground. Fog collection rates are typically 1–10 L m−2 of vertical collecting surface per day but can reach values of 30–40 L m−2 day−1. The presence of drizzle or light rain with the fog, coupled with 10 m s−1 winds, has produced collection rates as high as 300 L m−2 day−1. If a standard fog collector is installed at a site with wind speed measurements and a conventional rain gauge, a reasonable estimate can be made of the proportions of fog and rain being deposited on the vertical mesh panel. This information is fundamental to the understanding of acidic wet deposition at higher elevations and to comprehensive hydrological calculations in watersheds.
Cholera toxin feeding did not induce oral tolerance in mice and abrogated oral tolerance to an unrelated protein antigen. (1984)

The feeding of protein antigens to mice results in a state of tolerance when feeding is followed by parenteral immunization. Cholera toxin (CT) is a protein that has been used extensively as a potent oral immunogen for mucosal IgA responses, but CT feeding also stimulates a substantial plasma IgG antibody response. This latter finding prompted us to study whether or not CT induces oral tolerance. Mice were fed 5 mg keyhole limpet hemocyanin (KLH) or 10 micrograms CT at least twice before parenteral immunization with 1 microgram KLH or CT in alum i.p. Plasma and intestinal secretions were collected at intervals. The specific IgG or IgA antibody in the samples was measured by ELISA. Although KLH feeding did induce oral tolerance, CT feeding did not induce oral tolerance in any of three mouse strains tested or at any dose of CT given orally. The feeding of the B subunit of CT did not result in oral tolerance either. When both CT and KLH were fed together, CT was able to abrogate oral tolerance to KLH, an antigenically unrelated protein. Moreover, feeding CT along with KLH stimulated secretory IgA anti-KLH responses, whereas no such IgA responses were found when KLH was given alone. Thus, in these experiments with protein antigens, IgA immunization and oral tolerance were reciprocally linked and did not occur simultaneously. CT appears to abrogate oral tolerance and to stimulate secretory IgA responses by altering the regulatory environment in gut-associated lymphoid tissue, shifting it toward responsiveness.
Selection of an infectious bursal disease virus mutant with increased immunogenicity following passage under humoral immune pressure. (2001)

It is generally known that the pathogenicity of infectious bursal disease virus (IBDV) strains decreases following passage in cell culture. However, there is no information about the effect of passage under immune pressure on the phenotypic and molecular properties of IBDV. In the present study, a small plaque mutant virus with poor neutralization capability, but showing similar growth characteristics as the parental virus strain, QC2, was isolated after serial passage in Vero cells in presence of IBDV serotype 1 chicken polyclonal antiserum. This mutant virus showed reduced pathogenicity but enhanced immunogenicity compared to the parental virus. Sequence analysis of the non-coding regions of the genome revealed 4 and 3 nucleotide changes in the 3' non-coding regions of segments A and B, respectively, and none in the 5' non-coding regions. Restriction enzyme analysis of selected coding regions of the IBDV genome in both viruses revealed a loss of the PstI site in the VP2 region of the mutant virus. Selection of such mutant viruses by passaging under immune pressure may offer an improved method for developing safer and more effective attenuated vaccine strains against infectious bursal disease of chickens.
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor (2003)

Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
HEALING EFFECT OF TOPICAL NIFEDIPINE ON SKIN WOUNDS OF DIABETIC RATS (2003)

Non-healing foot ulcers in patients with diabetes are the leading causes of complications such as infection and amputation. Ulceration is the most common single precursor to amputation and has been identified as a causative factor in 85% of lower extremity amputations. It seems that poor outcomes are generally associated with infection, peripheral vascular disease and wounds of increasing depth. Nifedipine, a calcium channel blocker that is mainly used for the treatment of cardiovascular disorders has recently been used to treat wounds caused by peripheral vascular disorders. In present study topical Nifedipine 3% has been used to treat skin wounds in normal and diabetic rats. Effects of Nifedipine were evaluated in three different phases of wound healing process. In both experiments (normal and diabetic rats) topical Nifedipine significantly improved inflammatory phase. However, maturation phase was only significantly improved in diabetic rats. Nifedipine did not affect proliferation phase in either group significantly. Overall results of this study showed topical Nifedipine improved skin wound healing process in normal and diabetic rats.
COGNITIVE RADIO APPLICATIONS IN SOFTWARE DEFINED RADIO (2008)

The topic of cognitive radios has been garnering a great deal of attention in the past several years. Opinions regarding the level of sophistication necessary to qualify a system as cognitive vary widely, and discussions have ensued regarding this technology. The software defined radio forum is also involved and has working group activity in the area of cognitive radio. Some of the working group results are shown in this paper. The modern software defined radio is the heart of a cognitive radio. The applications executing on the radio distinguish a cognitive radio from a software-defined radio. Additional hardware in the form of sensors and actuators enables more cognitive radio applications. Some of the enabling technology is presented in this paper. Various artificial intelligence approaches to machine learning and decision making may be applied to the cognitive radio system. In this paper, a survey of related cognitive radio topics is reviewed and candidate application architectures are presented. Section 1 is an introduction to the basis of cognitive radios from a hardware, software, and artificial intelligence point of view. Section 2 discusses the sensors and actuators needed by a cognitive radio. Section 3 presents software architectures for radio learning and ultimately cognition. Section 4 is the heart of the paper and describes three candidate cognitive radio applications. Some future trends and conclusions are presented in Section 5.
Automatically classifying emails into activities (2006)

Email-based activity management systems promise to give users better tools for managing increasing volumes of email, by organizing email according to a user's activities. Current activity management systems do not automatically classify incoming messages by the activity to which they belong, instead relying on simple heuristics (such as message threads), or asking the user to manually classify incoming messages as belonging to an activity. This paper presents several algorithms for automatically recognizing emails as part of an ongoing activity. Our baseline methods are the use of message reply-to threads to determine activity membership and a naïve Bayes classifier. Our SimSubset and SimOverlap algorithms compare the people involved in an activity against the recipients of each incoming message. Our SimContent algorithm uses IRR (a variant of latent semantic indexing) to classify emails into activities using similarity based on message contents. An empirical evaluation shows that each of these methods provide a significant improvement to the baseline methods. In addition, we show that a combined approach that votes the predictions of the individual methods performs better than each individual method alone.
Functional Neuroimaging of Anxiety: A Meta-Analysis of Emotional Processing in PTSD, Social Anxiety Disorder, and Specific Phobia (2007)

This meta-analysis allowed us to synthesize often disparate findings from individual studies and thereby provide neuroimaging evidence for common brain mechanisms in anxiety disorders and normal fear. Effects unique to PTSD furthermore suggested a mechanism for the emotional dysregulation symptoms in PTSD that extend beyond an exaggerated fear response. Therefore, these findings help refine our understanding of anxiety disorders and their interrelationships.
Task Force on Recurrent Respiratory Papillomas: A Preliminary Report (1995)

A registry of patients with recurrent respiratory papillomas would benefit future research protocols and provide long-term follow-up of patients.
Evaluation of adaptive combination of 30‐channel head receive coil array data in 23Na MR imaging (2015)

(23) Na MRI can be markedly improved by using a 30-channel receive array and ADC reconstruction. The ADC reconstruction showed robust results for all measurements without the need for sensitivity maps.
An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans (2001)

Two small temporal RNAs (stRNAs), lin-4 and let-7, control developmental timing in Caenorhabditis elegans. We find that these two regulatory RNAs are members of a large class of 21- to 24-nucleotide noncoding RNAs, called microRNAs (miRNAs). We report on 55 previously unknown miRNAs in C. elegans. The miRNAs have diverse expression patterns during development: a let-7 paralog is temporally coexpressed with let-7; miRNAs encoded in a single genomic cluster are coexpressed during embryogenesis; and still other miRNAs are expressed constitutively throughout development. Potential orthologs of several of these miRNA genes were identified in Drosophila and human genomes. The abundance of these tiny RNAs, their expression patterns, and their evolutionary conservation imply that, as a class, miRNAs have broad regulatory functions in animals.
Synthesis and processing of human immunodeficiency virus type 1 envelope proteins encoded by a recombinant human adenovirus (1989)

A recombinant adenovirus was constructed by inserting the human immunodeficiency virus type 1 (HIV-1) envelope gene downstream from the early region 3 (E3) promoter of adenovirus type 5 (Ad5), replacing the coding sequences of E3. The recombinant virus replicated as efficiently as the parent virus in all cell lines tested. Human cells infected with the recombinant virus synthesized the HIV-1 envelope precursor gp160, which was efficiently processed to the envelope glycoproteins gp120 and gp41. A human T-lymphoblast line (Molt-4) infected with the recombinant virus expressed HIV-1 envelope glycoproteins on the cell surface, leading to syncytium formation. The envelope gene was expressed from the E3 promoter at early times after infection and at late times from the major late promoter. When cotton rats were infected with the recombinant virus, antibodies against the HIV-1 envelope glycoproteins could be expressed in an immunoreactive form by the recombinant adenovirus, further illustrating the usefulness of adenoviruses as expression vectors.
Electronic Bills of Lading and Functional Equivalence (1998)

&lt;p&gt;Merchants are increasingly using computers to facilitate international transactions, and many observers believe that full-fledged electronic commerce is nearing reality. At present, the business community uses a system known as electronic data interchange (EDI). Because EDI is both quick and efficient, the shipping industry is expected to benefit greatly from its adoption, particularly in the areas of bills of lading. Unfortunately, numerous technical and legal obstacles have slowed the introduction of EDI, with the chief impediment being the law's insistence on paper-based documentation.&lt;/p&gt;Recently, however, several significant steps have been taken to promote the use of EDI. The purpose of this paper is to briefly describe these developments.
The influence of walking mechanics and time on the results of proximal tibial osteotomy. (1990)

The current study describes the influence of the passage of time on the original findings. The mechanisms used by some patients to reduce loading at the knee (adduction moment) also were analyzed. We evaluated the gait of twenty-seven patients (thirty-two knees) who had had a proximal tibial osteotomy for a varus gonarthrosis. Twenty-four patients (twenty-eight knees) returned for follow-up at three to 8.9 years after the osteotomy. This is a follow-up to our original study (Prodromos et al.), which described a relationship between the magnitude of the adduction moment at the knee during walking and the outcome of proximal tibial osteotomy. The patients were divided into low and high adduction-moment groups on the basis of the magnitude of the adduction moment at the knee as measured preoperatively during walking. All fourteen patients in the low adduction-moment group and nine of the fourteen patients in the high adduction-moment group had a good or excellent result. The varus deformity recurred in ten patients in the high adduction-moment group and in three patients in the low adduction-moment group. All of the results degenerated over time. The adaptive mechanism that was used to lower the adduction moment during gait included shortening the stride and toeing-out. The significant correlation between the magnitudes of the inversion moment at the ankle and the adduction moment at the knee suggested that the toe-out gait reduced the adduction moment at the knee.(ABSTRACT TRUNCATED AT 250 WORDS)
Bitter-Tasting and Kokumi-Enhancing Molecules in Thermally Processed Avocado (Persea americana Mill.) (2010)

Sequential application of solvent extraction and RP-HPLC in combination with taste dilution analyses (TDA) and comparative TDA, followed by LC-MS and 1D/2D NMR experiments, led to the discovery of 10 C(17)-C(21) oxylipins with 1,2,4-trihydroxy-, 1-acetoxy-2,4-dihydroxy-, and 1-acetoxy-2-hydroxy-4-oxo motifs, respectively, besides 1-O-stearoyl-glycerol and 1-O-linoleoyl-glycerol as bitter-tasting compounds in thermally processed avocado (Persea americana Mill.). On the basis of quantitative data, dose-over-threshold (DoT) factors, and taste re-engineering experiments, these phytochemicals, among which 1-acetoxy-2-hydroxy-4-oxo-octadeca-12-ene was found with the highest taste impact, were confirmed to be the key contributors to the bitter off-taste developed upon thermal processing of avocado. For the first time, those C(17)-C(21) oxylipins exhibiting a 1-acetoxy-2,4-dihydroxy- and a 1-acetoxy-2-hydroxy-4-oxo motif, respectively, were discovered to induce a mouthfulness (kokumi)-enhancing activity in sub-bitter threshold concentrations.