gebhart/patrepeval huggingface dataset:
Identification and Validation of Candidate Soluble Biomarkers for Psoriatic Arthritis Using Quantitative Proteomics (2015)

There is a high prevalence of undiagnosed PsA in psoriasis patients; therefore identifying soluble biomarkers for PsA will help in screening psoriasis patients for appropriate referral to a rheumatologist. Potential PsA biomarkers likely originate in sites of inflammation, such as inflamed joints and skin, and subsequently enter systemic circulation. Therefore, we aimed to discover novel biomarkers, to facilitate PsA recognition in psoriasis patients. To achieve this objective, quantitative proteomic analyses of synovial fluid (SF) samples and skin biopsies obtained from PsA patients were performed. SF was obtained from swollen knee joints of 10 PsA patients, and age/sex matched early osteoarthritis (OA) controls. Likewise, skin biopsies were obtained from involved and uninvolved skin of 10 PsA, and 10 age/sex matched psoriasis patients without PsA (PsC). Using strong cation exchange chromatography, followed by tandem mass spectrometry, we characterized the proteomes of pooled SF and pooled skin samples. Extracted ion current intensities were used to calculate protein abundance ratios, and utilized to classify upregulated proteins. Selected reaction monitoring assays were developed to quantify these potential PsA markers in individual patient samples. Verified markers were subsequently measured in serum samples from 100 PsA, 100 PsC patients, and 100 healthy controls, using commercially available or in-house developed enzyme-linked immunosorbent assays. We quantified a total of 443 and 1922 proteins in SF and skin extracts, respectively, but only 17 proteins represented upregulated proteins in PsA SF, while 47 proteins were specifically elevated in PsA-derived skin. SRM verification confirmed that 12 and 8 proteins were indeed elevated in an independent set of PsA SF and involved PsA skin, respectively. Based on the fold change between PsA and controls, the associated P-values, and the cellular localization, we ranked the proteins, and selected the following putative markers for validation in the serum - M2BP, CD5L, MMP3, CRP, MPO, and ITGB5. Increased levels of CRP, M2BP, MMP3, and ITGB5 were independently associated with PsA, when compared to PsC. ROC analysis of this model showed an AUC of 0.851 [95% CI (0.799, 0.904)]. Thus, serum CRP, M2BP, and ITGB5 are potential biomarkers of PsA in patients with psoriasis.
General Concepts of Wrist Biomechanics and a View from Other Species (2008)

The carpus is one of the most complex joints in the extremities both in respect of its structure and its mechanics. In this article, an overview of the structural and biomechanical characteristics of the human carpus is presented first, followed by the author's observations on the structural characteristics of the carpal equivalents of a variety of animal species compared with those of the human carpus. Finally, the implications of these observations for the functional reconstruction of the hand are outlined.
18F PET imaging of cardiac sympathetic denervation with LMI1195, a new neuronal imaging agent (2010)

1701 Objectives Cardiac sympathetic denervation (CSD) assessed by 123I-metaiodobenzylguanidin (MIBG) imaging has been suggested to predict cardiac events including arrhythmia and death in heart failure patients (ADMIRE-HF trial). LMI1195 is a benzylguanidine derivative, like MIBG, but labeled with 18F to improve image quality and quantification by allowing PET imaging. This study evaluated if imaging with LMI1195 could be used to identify CSD. Methods Rabbit models of regional and systemic CSD were used. To develop regional CSD, a median sternotomy was performed and phenol (89% in liquid) was painted on the anterior and posterior walls of the left ventricle. To develop systemic denervation, the neurotoxin 6-hydroxydopamine (25 mg/kg on days 1, 2, 7 and 8) was administered intravenously. Two weeks following these procedures, rabbits were imaged with LMI1195 (~1.5 mCi, iv) using a microPET camera for 30 minutes. To ensure the denervation procedures did not result in perfusion changes, rabbits were also imaged with the 18F perfusion imaging agent BMS747158. Results In sham-denervated rabbits, cardiac images of LMI1195 showed clear myocardium with uniform radioactivity distribution. The radioactivity was low in the lung and liver and cleared rapidly in blood. In rabbits with systemic denervation, image based quantification indicated ~ 80% global reduction in heart uptake of LMI1195 compared to control animals. Similarly, regional denervation resulted in a marked reduction in LMI1195 uptake in the treated regions. In contrast, cardiac imaging with BMS747158 demonstrated well-perfused myocardium and no differences were observed between control and denervated rabbits. Conclusions Reduced LMI1195 heart uptake in CSD rabbits is due to impaired innervation, not to alterations in perfusion. Cardiac PET imaging with LMI1195 can be used for detection of CSD, like 123I-MIBG, but with improved image quality and quantification
A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary hepatocytes. (1994)

Retroviral vectors have been central components in many studies leading to human gene therapy. However, the generally low titers and inefficient infectivity of retroviral vectors in human cells have limited their use. We previously reported that the G protein of vesicular stomatitis virus can serve as the exclusive envelope protein component for one specific retroviral vector, LGRNL, that expresses vesicular stomatitis virus G. We now report a more useful general transient transfection scheme for producing very high-titer vesicular stomatitis virus G-enveloped pseudotypes from any Moloney murine leukemia-based retroviral vector without having to rely on the expression of the cytotoxic G protein from the retroviral vector itself. We also demonstrate very high efficiency of infection with a pseudotyped lacZ vector in primary mouse hepatocytes. We suggest that pseudotyped retroviral vectors carrying reporter genes will permit genetic studies in many previously inaccessible vertebrate and invertebrate systems. Furthermore, because these vectors represent retroviral vectors of sufficiently high titer to allow efficient direct retroviral-mediated in vivo gene transfer, we also suggest that pseudotyped vectors carrying potentially therapeutic genes will become useful to test the potential for in vivo gene therapy.
Chorismate mutase inhibitors: synthesis and evaluation of some potential transition-state analogs (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChorismate mutase inhibitors: synthesis and evaluation of some potential transition-state analogsPaul A. Bartlett, Yumi Nakagawa, Charles R. Johnson, Siegfried H. Reich, and Angela LuisCite this: J. Org. Chem. 1988, 53, 14, 3195–3210Publication Date (Print):July 1, 1988Publication History Published online1 May 2002Published inissue 1 July 1988https://pubs.acs.org/doi/10.1021/jo00249a013https://doi.org/10.1021/jo00249a013research-articleACS PublicationsRequest reuse permissionsArticle Views740Altmetric-Citations101LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
2017년 인보험 관련 판례 및 인보험 분쟁조정사례에 대한 검토 (2017)

이 논문은 2017년 선고된 인보험판결 중 중요하다고 생각되는 판결의 내용을 요약하고 이에 대하여 간략한 코멘트를 덧붙인 것이다. 수집대상은 보험계약법 총칙과 인보 험편 판결을 주된 대상으로 하였다. 아울러 손해보험판결 중에서 인보험에 대하여도 의미를 지닌다고 보여 지는 일부 판결을 포함하였다. 나아가 보험법 영역을 떠나 생명보험 산업과 관련이 깊은 일부...
Black-Box Superconducting Circuit Quantization (2012)

We present a semiclassical method for determining the effective low-energy quantum Hamiltonian of weakly anharmonic superconducting circuits containing mesoscopic Josephson junctions coupled to electromagnetic environments made of an arbitrary combination of distributed and lumped elements. A convenient basis, capturing the multimode physics, is given by the quantized eigenmodes of the linearized circuit and is fully determined by a classical linear response function. The method is used to calculate numerically the low-energy spectrum of a 3D transmon system, and quantitative agreement with measurements is found.
Impact of atomic-layer-deposited TiN on the gate oxide quality of W/TiN/SiO2/Si metal–oxide–semiconductor structures (2002)

We demonstrate the impact of atomic-layer-deposited TiN gate on the characteristics of W/TiN/SiO2/p-Si metal–oxide–semiconductor (MOS) systems. Damage-free gate oxide quality was attained with atomic-layer-deposition (ALD)–TiN as manifested by an excellent interface trap density (Dit) as low as ∼4×1010 eV−1 cm−2 near the Si midgap. ALD–TiN improved the Dit level of MOS systems on both thin SiO2 and high-permittivity (high-k) gate dielectrics. The leakage current of a MOS capacitor gated with ALD–TiN is remarkably lower than that with sputter-deposited TiN and poly-Si gate at the similar capacitance equivalent thickness (CET). Less chlorine content in ALD–TiN films appears to be pivotal in minimizing the CET increase against postmetal anneal and improving gate oxide reliability, paving a way for the direct metal gate process.
Optimal structure for microgrooved cooling fin for high-power LSI devices (1986)

We have evaluated the relationship between the cooling capability and the channel width with a constant pressure drop for microgrooved cooling fins, and found that there are optimal channel widths which give rise to the maximum allowable power density. There are also optimal microgrooved cooling fin structures in which enlarged channel widths are sufficient to cool high-power-consumption wafer-scale integrated chips with a pressure drop of only 200 kg/m2.
Changes in salted yoghurt during storage (2007)

Summary The study was conducted to determine the gross chemical composition and concentrations of free fatty acids (FFAs) and volatile compounds of salted yoghurt made from indigenous goat's milk. The effects of some chemical properties on sensory attributes of salted yoghurt were also studied. Changes in FFAs ( P &lt; 0.01) and volatile compounds were statistically significant ( P &lt; 0.05) during storage. The most abundant FFAs were 9‐octadecenoic (C 18:1 ), hexadecanoic (C 16 ), tetradecanoic (C 14 ) and decanoic (C 10 ) acids, respectively. Ethanol and ethyl acetate were principal volatile compounds through the end of storage period. The storage period affected the intensity of flavour attributes and overall flavour score of salted yoghurt ( P &lt; 0.05). According to factor analysis result, chemical compounds including volatiles, FFAs up to C 14 , tyrosine and titratable acidity, and flavour attributes explained 86.50% and 55.33% of total variance, respectively.
Identification of Cell Surface Targets through Meta-analysis of Microarray Data (2012)

High-resolution image guidance for resection of residual tumor cells would enable more precise and complete excision for more effective treatment of cancers, such as medulloblastoma, the most common pediatric brain cancer. Numerous studies have shown that brain tumor patient outcomes correlate with the precision of resection. To enable guided resection with molecular specificity and cellular resolution, molecular probes that effectively delineate brain tumor boundaries are essential. Therefore, we developed a bioinformatics approach to analyze micro-array datasets for the identification of transcripts that encode candidate cell surface biomarkers that are highly enriched in medulloblastoma. The results identified 380 genes with greater than a two-fold increase in the expression in the medulloblastoma compared with that in the normal cerebellum. To enrich for targets with accessibility for extracellular molecular probes, we further refined this list by filtering it with gene ontology to identify genes with protein localization on, or within, the plasma membrane. To validate this meta-analysis, the top 10 candidates were evaluated with immunohistochemistry. We identified two targets, fibrillin 2 and EphA3, which specifically stain medulloblastoma. These results demonstrate a novel bioinformatics approach that successfully identified cell surface and extracellular candidate markers enriched in medulloblastoma versus adjacent cerebellum. These two proteins are high-value targets for the development of tumor-specific probes in medulloblastoma. This bioinformatics method has broad utility for the identification of accessible molecular targets in a variety of cancers and will enable probe development for guided resection.
The Proteasome as a Lipopolysaccharide-Binding Protein in Macrophages: Differential Effects of Proteasome Inhibition on Lipopolysaccharide-Induced Signaling Events (2003)

We have developed a novel LPS probe using a highly purified and homogenous preparation of [(3)H] Escherichia coli LPS from the deep rough mutant, which contains a covalently linked, photoactivable 4-p-(azidosalicylamido)-butylamine group. This cross-linker was used to identify the LPS-binding proteins in membranes of the murine-macrophage-like cell line RAW 264.7. The alpha-subunit (PSMA1 C2, 29.5 kDa) and the beta-subunit (PSMB4 N3, 24.36 kDa) of the 20S proteasome complex were identified as LPS-binding proteins. This is the first report demonstrating LPS binding to enzymes such as the proteasome subunits. Functionally, LPS enhanced the chymotrypsin-like activity of the proteasome to degrade synthetic peptides in vitro and, conversely, the proteasome inhibitor lactacystin completely blocked the LPS-induced proteasome's chymotrypsin activity as well as macrophage TNF-alpha secretion and the expression of multiple inflammatory mediator genes. Lactacystin also completely blocked the LPS-induced expression of Toll-like receptor 2 mRNA. In addition, lactacystin dysregulated mitogen-activated protein kinase phosphorylation in LPS-stimulated macrophages, but failed to inhibit IL-1 receptor-associated kinase-1 activity. Importantly, lactacystin also prevented LPS-induced shock in mice. These data strongly suggest that the proteasome complex regulates the LPS-induced signal transduction and that it may be an important therapeutic target in Gram-negative sepsis.
Chemical Approaches to Glycobiology (2010)

Glycans are ubiquitous components of all organisms. Efforts to elucidate glycan function and to understand how they are assembled and disassembled can reap benefits in fields ranging from bioenergy to human medicine. Significant advances in our knowledge of glycan biosynthesis and function are emerging, and chemical biology approaches are accelerating the pace of discovery. Novel strategies for assembling oligosaccharides, glycoproteins, and other glycoconjugates are providing access to critical materials for interrogating glycan function. Chemoselective reactions that facilitate the synthesis of glycan-substituted imaging agents, arrays, and materials are yielding compounds to interrogate and perturb glycan function and dysfunction. To complement these advances, small molecules are being generated that inhibit key glycan-binding proteins or biosynthetic enzymes. These examples illustrate how chemical glycobiology is providing new insight into the functional roles of glycans and new opportunities to interfere with or exploit these roles.
Intra-articular therapy in osteoarthritis (2003)

The medical literature was reviewed from 1968-2002 using Medline and the key words "intra-articular" and "osteoarthritis" to determine the various intra-articular therapies used in the treatment of osteoarthritis. Corticosteroids and hyaluronic acid are the most frequently used intra-articular therapies in osteoarthritis. Other intra-articular substances such as orgotein, radiation synovectomy, dextrose prolotherapy, silicone, saline lavage, saline injection without lavage, analgesic agents, non-steroidal anti-inflammatory drugs, glucosamine, somatostatin, sodium pentosan polysulfate, chloroquine, mucopolysaccharide polysulfuric acid ester, lactic acid solution, and thiotepa cytostatica have been investigated as potentially therapeutic in the treatment of arthritic joints. Despite the lack of strong, convincing, and reproducible evidence that any of the intra-articular therapies significantly alters the progression of osteoarthritis, corticosteroids and hyaluronic acid are widely used in patients who have failed other therapeutic modalities for lack of efficacy or toxicity. As a practical approach for a knee with effusion, steroid injections should be considered while the presence of symptomatic "dry" knees may favour the hyaluronic acid approach. The virtual absence of serious side effects, coupled with the perceived benefits, make these approaches attractive.
Molecular Characterization of Stool Microbiota in HIV-Infected Subjects by Panbacterial and Order-Level 16S Ribosomal DNA (rDNA) Quantification and Correlations With Immune Activation (2011)

These data represent the first report of quantitative molecular and cellular correlations between total/universal and order-level gut bacterial populations and gastrointestinal-associated lymphoid tissue levels of immune activation in HIV-infected subjects. The correlations between lower overall 16S rDNA levels and tissue immune activation suggest that the gut microbiome may contribute to immune activation and influence HIV progression.
The Stanford FLASH multiprocessor (1994)

The FLASH multiprocessor efficiently integrates support for cache-coherent shared memory and high-performance message passing, while minimizing both hardware and software overhead. Each node in FLASH contains a microprocessor, a portion of the machine's global memory, a port to the interconnection network, an I/O interface, and a custom node controller called MAGIC. The MAGIC chip handles all communication both within the node and among nodes, using hardwired data paths for efficient data movement and a programmable processor optimized for executing protocol operations. The use of the protocol processor makes FLASH very flexible --- it can support a variety of different communication mechanisms --- and simplifies the design and implementation.This paper presents the architecture of FLASH and MAGIC, and discusses the base cache-coherence and message-passing protocols. Latency and occupancy numbers, which are derived from our system-level simulator and our Verilog code, are given for several common protocol operations. The paper also describes our software strategy and FLASH's current status.
Termination of early human pregnancy with RU 486 (mifepristone) and the prostaglandin analogue sulprostone: a multi-centre, randomized comparison between two treatment regimens (1989)

A multi-centre, randomized trial was conducted to compare the efficacy and side-effects of two combination regimens of the antiprogestin RU 486 and the intramuscular PGE2 analogue sulprostone for termination of early pregnancy (amenorrhoea up to 49 days). Women in the 3-day group (n = 125) received 25 mg RU 486 twice daily for 3 days plus a single injection of 0.25 mg sulprostone in the morning of the third day of antiprogestin treatment. In the 4-day group (n = 126), RU 486 was given for 4 days and the sulprostone injection in the morning of the fourth day. Treatment outcome in the two groups was similar. Overall, 88.8% had a complete abortion, 6.8% an incomplete abortion and 2.4% were treatment failures; in the remaining 2% treatment outcome could not be determined. Only three of the six women with treatment failure still had detectable fetal heart activity when the pregnancy was terminated by vacuum aspiration two weeks after the start of treatment. Five of the 17 interventions for incomplete abortion were carried out as emergency procedures because of heavy bleeding; two of these five women were given a blood transfusion. The majority of the curettages (10/17) were performed in one centre. If the data from this centre and the women with undetermined treatment outcome were excluded, the rates for complete abortion, incomplete abortion and treatment failure in the remaining six centres were 93.6, 3.7 and 2.7%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Prediction of risk of death and myocardial infarction in the six months after presentation with acute coronary syndrome: prospective multinational observational study (GRACE) (2006)

This risk prediction tool uses readily identifiable variables to provide robust prediction of the cumulative six month risk of death or myocardial infarction. It is a rapid and widely applicable method for assessing cardiovascular risk to complement clinical assessment and can guide patient triage and management across the spectrum of patients with acute coronary syndrome.
Eliminating Preventable Death on the Battlefield (2011)

A command-directed casualty response system that trains all personnel in Tactical Combat Casualty Care and receives continuous feedback from prehospital trauma registry data facilitated Tactical Combat Casualty Care performance improvements centered on clinical outcomes that resulted in unprecedented reduction of killed-in-action deaths, casualties who died of wounds, and preventable combat death. This data-driven approach is the model for improving prehospital trauma care and casualty outcomes on the battlefield and has considerable implications for civilian trauma systems.
Transgenic mice generated by pronuclear injection of a yeast artificial chromosome (1992)

Transgenic mice have become invaluable for analysing gene function and regulation in vivo. However, the size of constructs injected has been limited by the cloning capacity of conventional vectors, a constraint that could be overcome with yeast artificial chromosomes (YACs). We investigated the feasibility of making transgenic mice with YACs by pronuclear injection of a small YAC carrying a gene encoding tyrosinase. Use of a vector with a conditional centromere allowed fifteenfold amplification of the YAC in yeast and its recovery in high yield. The albino phenotype of the recipient mice was rescued demonstrating the correct expression of the tyrosine gene from the construct. Furthermore, the telomeric sequences added by the yeast integrated into the mouse genome and did not reduce efficiency of integration. Using this technique future experiments with longer YACs will allow the expression of gene complexes such as Hox and the globin gene clusters to be analysed in transgenic animals.