gebhart/patrepeval huggingface dataset:
Five-parameter fluorescence imaging: wound healing of living Swiss 3T3 cells. (1987)

Cellular functions involve the temporal and spatial interplay of ions, metabolites, macromolecules, and organelles. To define the mechanisms responsible for completing cellular functions, we used methods that can yield both temporal and spatial information on multiple physiological parameters and chemical components in the same cell. We demonstrated that the combined use of selected fluorescent probes, fluorescence microscopy, and imaging methods can yield information on at least five separate cellular parameters and components in the same living cell. Furthermore, the temporal and spatial dynamics of each of the parameters and/or components can be correlated with one or more of the others. Five parameters were investigated by spectrally isolating defined regions of the ultraviolet, visible, and near-infrared spectrum based on five distinct fluorescent probes. The parameters included nuclei (Hoechst 33342), mitochondria (diIC1-[5] ), endosomes (lissamine rhodamine B-dextran), actin (fluorescein), and the cell volume Cy7-dextran). Nonmotile, confluent Swiss 3T3 cells did not show any detectable polarity of cell shape, or distribution of nuclei, endosomes, or mitochondria. These cells also organized a large percentage of the actin into stress fibers. In contrast, cells migrating into an in vitro wound exhibited at least two stages of reorganization of organelles and cytoplasm. During the first 3 h after wounding, the cells along the edge of the wound assumed a polarized shape, carried the nuclei in the rear of the cells, excluded endosomes and mitochondria from the lamellipodia, and lost most of the highly organized stress fibers. The cell showed a dramatic change between 3 and 7 h after producing the wound. The cells became highly elongated and motile; both the endosomes and the mitochondria penetrated into the lamellipodia, while the nuclei remained in the rear and the actin remained in less organized structures. Defining the temporal and spatial dynamics and interplay of ions, contractile proteins, lipids, regulatory proteins, metabolites, and organelles should lead to an understanding of the molecular basis of cell migration, as well as other cellular functions.
Nucleophilic reactions of pyridines and imidazoles with vinyl and aromatic halides (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleophilic reactions of pyridines and imidazoles with vinyl and aromatic halidesAndrew S. Koch, Amy S. Feng, Traci A. Hopkins, and Andrew StreitwieserCite this: J. Org. Chem. 1993, 58, 6, 1409–1414Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/jo00058a022https://doi.org/10.1021/jo00058a022research-articleACS PublicationsRequest reuse permissionsArticle Views538Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Dermal Fillers (2004)

In today's busy and demanding world, we no longer have the luxury of taking weeks to recover from a surgical procedure and are more frequently seeking quicker alternatives. The use of dermal fillers meets this need but in no way replaces a surgical intervention. Previously, bovine collagen was the only approved dermal filler. However, today there are several options available including a human collagen, a variety of hyaluronic acids, and a permanent injectable product. Each of the products has different uses, indications, and adverse reactions. The experienced injector now has a wider selection of products from which to choose to ensure that the patient receives what is best suited for his or her particular situation. These new products are becoming increasingly popular, due to acceptability and affordability, but are not without potential complications and adverse reactions. This article discusses the use of Cosmoderm/Cosmoplast, Hylaform, Restylane/Perlane, and Artecoll dermal fillers.
World Science Report 1998 (1998)

The third in a biennial series published by UNESCO, the World Science Report 1998 looks at the current state of science around the globe through the eyes of an international team of experts. Who is carrying out research and development, where and with what means? What has changed over the past couple of years? Informative and thought-provoking, each essay describes how research and development are organized in a given region or group of countries and identifies and discusses emerging trends in research and science education, including region- or country-specific issues. Wherever possible, the author&apos;s opinion is backed up by numerical data presented in an easily accessible form as tables or diagrams. The second part of the Report examines three contemporary issues that have in common their global nature and ramifications for society as a whole. Separate chapters look at how science is helping to safeguard our two most basic commodities - food and water - in a context of rapid demographic growth and environmental stress. Globalization, a leitmotiv throughout the first part of the Report, is also the subject of the concluding chapter, which discusses its impact on scientific communities in the developing countries.
[Clinical analysis of 206 pregnant patients with multiple organ dysfunction syndrome]. (2007)

Primary diseases leading to pregnancy complicated with MODS are mainly due to obstetric factors, accounting for 65.05%. However, dysfunction of different organs can result in a significant difference in mortality, mortality from primary diseases of the heart and brain were higher. It is suggested that preventative and therapeutic measures should be taken according to different primary disease organs.
Learning Representations and Generative Models for 3D Point Clouds (2017)

Three-dimensional geometric data offer an excellent domain for studying representation learning and generative modeling. In this paper, we look at geometric data represented as point clouds. We introduce a deep AutoEncoder (AE) network with state-of-the-art reconstruction quality and generalization ability. The learned representations outperform existing methods on 3D recognition tasks and enable shape editing via simple algebraic manipulations, such as semantic part editing, shape analogies and shape interpolation, as well as shape completion. We perform a thorough study of different generative models including GANs operating on the raw point clouds, significantly improved GANs trained in the fixed latent space of our AEs, and Gaussian Mixture Models (GMMs). To quantitatively evaluate generative models we introduce measures of sample fidelity and diversity based on matchings between sets of point clouds. Interestingly, our evaluation of generalization, fidelity and diversity reveals that GMMs trained in the latent space of our AEs yield the best results overall.
Early detection of renal allograft rejection by serial monitoring of serum C-reactive protein. (1984)

Etude chez 15 receveurs. La surveillance du rapport OKT4/OKT8 (sous-populations de lymphocytes) est utile mais le dosage quotidien de la proteine C-reactive permet une prevision plus precise des episodes de rejet
Degradation of structurally different non-digestible oligosaccharides by intestinal bacteria: glycosylhydrolases of Bifidobacterium adolescentis. (2000)

Non-digestible oligosaccharides (NDOs) are oligosaccharides, which resist digestion in the upper gastrointestinal tract, and which are fermented in the colon by intestinal bacteria. Some NDOs are considered bifidogenic, meaning that they selectively stimulate the growth of bifidobacteria in the colon microbiota. The degradative fermentation of structurally different oligosaccharides by intestinal bacteria was studied in this thesis, in order to establish the potentially bifidogenic effects of various types of NDOs. Structurally different oligosaccharides were produced using different routes. Arabino-, (arabino-)galacto-, (arabino-)xylo-, galacturono-, and rhamnogalacturono- oligosaccharides were derived by enzymatic hydrolysis of plant polysaccharides. Through transglycosylation reactions using glycosidases, transgalactooligosaccharides of theα- andβ-glycosyl linkage type were obtained.The chemical structure of the oligosaccharides clearly influenced their fermentation behaviour. It was concluded that species belonging to different groups, so not only bifidobacteria, have the capability of hydrolysing these oligosaccharides. Bi. adolescentis, being a major bifidobacterial species of the adult intestinal microflora, was able to utilise a wide range of oligosaccharides showing its wide range of glycosidases. Two novel arabinoxylan degrading enzymes were purified from Bi. adolescentis and these enzymes in combination with aβ-xylosidase are involved in the complete degradation of arabinoxylooligosaccharides. For the utilisation ofα-galactooligosaccharides Bi. adolescentis produced anα-galactosidase. Thisα-galactosidase was characterised as a retaining glycosidase and was used for the production of new types ofα-galactooligosaccharides. Theseα-1→6 linked-galactooligosaccharides could be utilised by various strains belonging to bifidobacteria and lactobacilli. Upon growth of Bi. adolescentis on transgalactooligosaccharides (TOS) a novelβ-galactosidase was produced, involved in the degradation of TOS. It was speculated that this enzyme was membrane or cell wall associated. After growth of Bi. adolescentis on TOS or on hydrolysed arabinogalactan theβ-galactosidase production of Bi. adolescentis increased compared to growth on galactose and this increasedβ-galactosidase activity could be linked to increased activity towards both polymeric and oligomeric galactan. In vivo dietary intervention with TOS also resulted in increased levels of v-galactosidase activity in feces. Although the nature and specificity of theβ-galactosidase is not yet known it can be concluded that glycosidase activity of the intestinal bacteria might be a useful biomarker of the colonic metabolic activity.
AN ANTIGENIC ANALYSIS OF RESPIRATORY SYNCYTIAL VIRUS ISOLATES BY A PLAQUE REDUCTION NEUTRALIZATION TEST1 (1966)

Journal Article AN ANTIGENIC ANALYSIS OF RESPIRATORY SYNCYTIAL VIRUS ISOLATES BY A PLAQUE REDUCTION NEUTRALIZATION TEST Get access H. V. COATES, H. V. COATES Search for other works by this author on: Oxford Academic PubMed Google Scholar D. W. ALLING, D. W. ALLING Search for other works by this author on: Oxford Academic PubMed Google Scholar R. M. CHANOCK R. M. CHANOCK Search for other works by this author on: Oxford Academic PubMed Google Scholar American Journal of Epidemiology, Volume 83, Issue 2, March 1966, Pages 299–313, https://doi.org/10.1093/oxfordjournals.aje.a120586 Published: 01 March 1966 Article history Received: 23 July 1965 Published: 01 March 1966
Regulation of Herpesvirus Macromolecular Synthesis I. Cascade Regulation of the Synthesis of Three Groups of Viral Proteins (1974)

Based on evidence that 50% of herpes simplex 1 DNA is transcribed in HEp-2 cells in the absence of protein synthesis we examined the order and rates of synthesis of viral polypeptides in infected cells after reversal of cycloheximide- or puromycin-mediated inhibition of protein synthesis. These experiments showed that viral polypeptides formed three sequentially synthesized, coordinately regulated groups designated alpha, beta, and gamma. Specifically: (i) The alpha group, containing one minor structural and several nonstructural polypeptides, was synthesized at highest rates from 3 to 4 h postinfection in untreated cells and at diminishing rates thereafter. The beta group, also containing minor structural and nonstructural polypeptides, was synthesized at highest rates from 5 to 7 h and at decreasing rates thereafter. The gamma group containing major structural polypeptides was synthesized at increasing rates until at least 12 h postinfection. (ii) The synthesis of alpha polypeptides did not require prior infected cell protein synthesis. In contrast, the synthesis of beta polypeptides required both prior alpha polypeptide synthesis as well as new RNA synthesis, since the addition of actinomycin D immediately after removal of cycloheximide precluded beta polypeptide synthesis. The function supplied by the alpha polypeptides was stable since interruption of protein synthesis after alpha polypeptide synthesis began and before beta polypeptides were made did not prevent the immediate synthesis of beta polypeptides once the drug was withdrawn. The requirement of gamma polypeptide synthesis for prior synthesis of beta polypeptides seemed to be similar to that of beta polypeptides for prior synthesis of the alpha group. (iii) The rates of synthesis of alpha polypeptides were highest immediately after removal of cycloheximide and declined thereafter concomitant with the initiation of beta polypeptide synthesis; this decline in alpha polypeptide synthesis was less rapid in the presence of actinomycin D which prevented the appearance of beta and gamma polypeptides. The decrease in rates of synthesis of beta polypeptides normally occurring after 7 h postinfection was also less rapid in the presence of actinomycin D than in its absence, whereas ongoing synthesis of gamma polypeptides at this time was rapidly reduced by actinomycin D. (iv) Inhibitors of DNA synthesis (cytosine arabinoside or hydroxyurea) did not prevent the synthesis of alpha, beta, or gamma polypeptides, but did reduce the amounts of gamma polypeptides made.
Long resonator micromachined tunable GaAs-AlAs Fabry-Perot filter (1997)

We present novel concepts for tunable optical filters. Long resonant cavities of about 30-μm length have been realized with two-chip designs. GaAs technology has been applied to filters that are designed for the use in dense wavelength-division multiplexing (WDM) at wavelengths around 1550 nm. A finesse of 46.7, a full-width at half-maximum (FWHM) of 1.2 nm and electrostatic tuning over a range of 103 nm with an applied voltage of 35 V has been achieved. An alternative tuning concept that allows to tune the resonator length 4 μm by heating Ni-Cr resistors placed on the suspending beams of a membrane with an applied voltage of 2.7 V has been realized.
Quantifying Admissible Undersampling for Sparsity-Exploiting Iterative Image Reconstruction in X-Ray CT (2012)

Iterative image reconstruction with sparsity-exploiting methods, such as total variation (TV) minimization, investigated in compressive sensing claim potentially large reductions in sampling requirements. Quantifying this claim for computed tomography (CT) is nontrivial, because both full sampling in the discrete-to-discrete imaging model and the reduction in sampling admitted by sparsity-exploiting methods are ill-defined. The present article proposes definitions of full sampling by introducing four sufficient-sampling conditions (SSCs). The SSCs are based on the condition number of the system matrix of a linear imaging model and address invertibility and stability. In the example application of breast CT, the SSCs are used as reference points of full sampling for quantifying the undersampling admitted by reconstruction through TV-minimization. In numerical simulations, factors affecting admissible undersampling are studied. Differences between few-view and few-detector bin reconstruction as well as a relation between object sparsity and admitted undersampling are quantified.
The A2a/A2breceptor antagonist ZM-241385 blocks the cardioprotective effect of adenosine agonist pretreatment in in vivo rat myocardium (2006)

There is increasing evidence for interactions among adenosine receptor subtypes in the brain and heart. The purpose of this study was to determine whether the adenosine A(2a) receptor modulates the infarct size-reducing effect of preischemic administration of adenosine receptor agonists in intact rat myocardium. Adult male rats were submitted to in vivo regional myocardial ischemia (25 min) and 2 h reperfusion. Vehicle-treated rats were compared with rats pretreated with the A(1) agonist 2-chloro-N(6)-cyclopentyladenosine (CCPA, 10 mug/kg), the nonselective agonist 5'-N-ethylcarboxamidoadenosine (NECA, 10 mug/kg), or the A(2a) agonist 2-[4-(2-carboxyethyl)phenethylamino]-5'-N-methylcarboxamidoadenosine (CGS-21680, 20 mug/kg). Additional CCPA- and NECA-treated rats were pretreated with the A(1) antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 100 mug/kg), the A(2a)/A(2b) antagonist 4-(-2-[7-amino-2-{2-furyl}{1,2,4}triazolo{2,3-a} {1,3,5}triazin-5-yl-amino]ethyl)phenol (ZM-241385, 1.5 mg/kg) or the A(3) antagonist 3-propyl-6-ethyl-5[(ethylthio)carbonyl]-2-phenyl-4-propyl-3-pyridine carboxylate (MRS-1523, 2 mg/kg). CCPA and NECA reduced myocardial infarct size by 50% and 35%, respectively, versus vehicle, but CGS-21680 had no effect. DPCPX blunted the bradycardia associated with CCPA and NECA, whereas ZM-241385 attenuated their hypotensive effects. Both DPCPX and ZM-241385 blocked the protective effects of CCPA and NECA. The A(3) antagonist did not alter the hemodynamic effects of CCPA or NECA, nor did it alter adenosine agonist cardioprotection. None of the antagonists alone altered myocardial infarct size. These findings suggest that although preischemic administration of an A(2a) receptor agonist does not induce cardioprotection, antagonism of the A(2a) and/or the A(2b) receptor blocks the cardioprotection associated with adenosine agonist pretreatment.
The Synthesis of 5-Alkylaminomethylthieno[2,3-b]pyrrole-5-sulfonamides (1989)

Novel Z-aminom~thylthienor?,3-hIpyrrole-5-sulfonamides have been prepared by direct chlorosulfonationlamidation of N-protected intermediates.Aryi sulfonamides have recently been shown t o be effective agents for reducing the intraocular pressure in rabbits after topical and are thus under clinical ~t u d y in the treatment of ocular hypertension.The mechanism of action of these molecules appears t o involve inhihition of carhonic anhylrase wit+ resultant diminutin in aqueous humor formation.'"5 4 s part of our program t o develop potent, topically effective carbonic anhydrare inhibitorsh1' we wish to report the synthesis of novel, water soluble amiwal!<yl derivatives of tYenol2,3-blpyrrole-5-sulfonamides.This effort employs a novel protection-deprutection scheme for the pyrrole nitrogen and a highlv efficient electrophilic aromatic chlorosulfanatin as t+e key step.Among t'le various arvl sulfonamides which have shown potent inhibition of carbonic anhyrlrase a c t i v i t ~, " ~" 8 the imidazor2,1-b]thia4iarole sulfonamides I were of interest t o us hecause of their pharmacologic profile.Specifically we were interested in the effect on biological activity of modifying the nitrogen atorn rite of a fused r5.51 heterocycle to be a hydrogen donor moiety rather than the acceptor that it is in I.We therefore indertook t'x preparation of tilieno!2,3-bIpyrrale-5-s~~lfonamides lla and Ilb, which possess an arninoalkyl function to enhanrr water roluhility.Although the parent thieno[2,3-hlpyrrole is not stable t o air or it war expected that the required sulfonamide 9 should be an isolable mmpaurnd due to the presence of appropriate electron-withdrawing fimctiorrality.This has proven to be t5e case and the synthetic patllway t o this rystem is shown in Scheme 1.
Ambiguities on convolutions with applications to phase retrieval (2016)

In this work we characterize all ambiguities of the convolution on two fixed finite-dimensional complex vector spaces. It will be shown that the convolution ambiguities correspond to factorization ambiguities in the z-domain, which are generated by swapping their zeros. We use this polynomial description to show a deterministic version of a recently introduced masked Fourier phase retrieval design. A semidefinite program can be used to recover exactly the noise-free input signals if they share no common factors. Then, we reformulate the problem as a deterministic blind deconvolution with knowledge of their autocorrelations. Moreover, numerically simulations show robustness against additive noise.
A STUDY ON THE MECHANISM OF ACUTE HEPATIC NECROSIS FOLLOWING PARTIAL RESECTION OF THE LIVER (1987)

Endotoxin was injected intravenously into rats 48 hours after 70% hepatectomy. The remaining hepatic tissue demonstrated marked hemorrhagic necrosis in high frequency. However, hepatic lesions were slight in other groups such as the group in which heparin was administered simultaneously with injection of endotoxin 48 hours after 70% hepatectomy and the group in which endotoxin was injected 10 days after 70% hepatectomy. Defining of this marked necrosis to be a univisceral Shwartzman reaction in the liver may be justified from (i) conformity of experimental manipulations to those employed in the Shwartzman reaction, (ii) macroscopical as well as histological evidence, (iii) formation of microthrombi in hepatic lesions, (iv) inhibition of occurrence of the reaction by heparin, and (v) absence of any remarkable change in other organs. We concluded that the hepatic regenerative state might correspond theoretically to a state of preparedness for the Shwartzman reaction.
Intravitreous Delivery of Dexamethasone Sodium m-Sulfobenzoate from Poly(DL-Lactic Acid) Implants. (1998)

Biodegradable intravitreal rod-shaped implants containing dexamethasone sodium m-sulfobenzoate (DMSB) were prepared from blends of poly(DL-lactic acid) (PLA) with number-average molecular weight 2000 (PLA2000) and 4000 (PLA4000). The effect of the fraction of PLA2000 on the release of DMSB from the implant was investigated after implantation in the vitreous body of rabbit eyes. After the initial burst, the drug was released slowly from the blended PLA implants with a PLA2000 fraction of below 30 wt% in normal eyes within a period of 28 d. For the implants with a higher PLA2000 fraction of over 50 wt%, the drug was released following approximately first order kinetics. In the vitrectomized eyes, the release of DMSB from the PLA2000/PLA4000 (5/5) implant was 2.5 times more rapid than in normal eyes, and the clearance of drug was also appreciably accelerated as compared with that in normal eyes.