gebhart/patrepeval huggingface dataset:
Induction of tumor-reactive CTL from peripheral blood and tumor-infiltrating lymphocytes of melanoma patients by in vitro stimulation with an immunodominant peptide of the human melanoma antigen MART-1. (1995)

MART-1 is an Ag expressed on melanomas and melanocytes, and is recognized by the majority of HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes (TIL) from melanoma patients. In the present study we have analyzed 10 potential 9-mer epitopes containing the HLA-A2.1 binding motifs for their ability to induce melanoma-specific T cell lines. Antimelanoma CTL could be generated only with MART-1(27-35) peptide, which has been previously shown to be recognized by a majority of HLA-A2-restricted TIL. Anti-MART-1(35-43)-specific CTL could also be induced, but these T cells did not recognize melanoma cells. MART-1(27-35)-specific CTL could be effectively generated from a total of 11 of 12 PBL and from 3 of 3 TIL derived from HLA-A2+ melanoma patients, as well as from 2 of 4 PBL from HLA-A2+ healthy donors by in vitro stimulation with autologous PBMC pulsed with the synthetic MART-1(27-35) peptide. These CTL lines specifically lysed and release cytokines (TNF-alpha, IFN-gamma, and GM-CSF) in response to T2 cells pulsed with MART-1(27-35), as well as to HLA-A2+ MART-1+ melanoma cells. CTL generated with MART-1(27-35) also lysed uncultured HLA-A2+ melanoma cells derived from tumor biopsies, indicating that this MART-1 epitope is likely to be expressed in association with HLA-A2 on the surface of tumor cells in vivo. CTL lines generated with MART-1(27-35) mediated 25- to 100-fold higher lytic activity than MART-1-reactive CTL grown from TIL in the presence of high dose IL-2. These results demonstrate that MART-1(27-35) peptide may represent an ideal candidate for Ag-specific immunotherapy in melanoma patients.
Targeting of surface alpha-enolase inhibits the invasiveness of pancreatic cancer cells (2015)

Pancreatic Ductal Adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by rapid progression, invasiveness and resistance to treatment. We have previously demonstrated that most PDAC patients have circulating antibodies against the glycolytic enzyme alpha-enolase (ENO1), which correlates with a better response to therapy and survival. ENO1 is a metabolic enzyme, also expressed on the cell surface where it acts as a plasminogen receptor. ENO1 play a crucial role in cell invasion and metastasis by promoting plasminogen activation into plasmin, a serine-protease involved in extracellular matrix degradation. The aim of this study was to investigate the role of ENO1 in PDAC cell invasion. We observed that ENO1 was expressed on the cell surface of most PDAC cell lines. Mouse anti-human ENO1 monoclonal antibodies inhibited plasminogen-dependent invasion of human PDAC cells, and their metastatic spreading in immunosuppressed mice was inhibited. Notably, a single administration of Adeno-Associated Virus (AAV)-expressing cDNA coding for 72/1 anti-ENO1 mAb reduced the number of lung metastases in immunosuppressed mice injected with PDAC cells. Overall, these data indicate that ENO1 is involved in PDAC cell invasion, and that administration of an anti-ENO1 mAb can be exploited as a novel therapeutic option to increase the survival of metastatic PDAC patients.
Othello Syndrome Secondary to Subthalamic Deep Brain Stimulation in Parkinson’s Disease (2017)

Deep brain stimulation (DBS) is established method of Parkinson’s disease(PD) treatment. The stimulation of the limbic portion of subthalamic nucleus (STN) might devise psychotic symptoms. PD psychosis may be related also to drug intake, especially dopamine agonists(DA). Authors present 36-year old PD patient treated with STN DBS with delusions of the marital infidelity that appeared 3 months after initiation of the stimulation and confining of l-dopa and DA to zero. Those symptoms were identified as Othello-like syndrome (OLS) and were successfully treated with both quetiapine and reprogramming of the stimulation. At one-year follow-up no recurrence of the psychotic symptoms were noted. The patient required no pharmacotherapy.
The Transformation of Private Liberal Arts Colleges (1991)

In the early 1980s, private liberal arts colleges were headed for serious trouble. Financial distress and declining enrollments made many closures seem inevitable. Yet these gloomy forecasts failed to materialize. Enrollments did not decline and are projected to remain stable during the next decade. Why? One answer may be institutional transformation. This article examines five private liberal arts colleges that inaugurated fundamental changes in management and organization during the 1980s, thus averting anticipated crises and apparently ensuring a healthy future.
Service parts logistics: Modeling, analysis and application (1998)

Service parts logistics is concerned with providing support through service parts availability to the after-sales service operations of equipment manufacturers. This dissertation studies strategies and methodologies for managing service parts logistics systems. The approach is to combine extensive field study with rigorous modeling analysis. The research was motivated directly by a project with a major US electronic equipment manufacturer to improve the operations of their world-wide service parts logistics system. The first part of the dissertation presents our preliminary study of the company's service parts logistics system. We build two simple models (one for consumables and the other for repairables) to approximate the company's complex system and to show their effectiveness in providing improved policies. Through this phase of study, we draw important insights regarding applications of operational models to real-world systems and abstract research problems. These new problems are then rigorously analyzed in the next two parts of the dissertation. The second part of the dissertation studies a two-echelon system (a single central repair depot and multiple local inventory stocking centers) for repairables with local-center-dependent depot replenishment lead times. We characterize the system by deriving the various exact performance metrics and then analyze system optimization problems. The following two features in the solution structure differentiate our model fundamentally from those in the literature: (1) the random delays at the depot of local center replenishment orders are different from center to center; (2) the lead time variances have a direct effect on system performance. The third part of the dissertation considers a two-echelon system for repairables with two classes of customer service: an emergency class, where a customer demand needs to be satisfied immediately upon its arrival, and a non-emergency class, where a customer demand needs not to be satisfied until a due date. Using both transient and steady state analysis approaches, we derive exact system performance metrics and characterize various properties. We then carry out numerical studies, based on company data, to quantify and to compare system improvement potential over company practice.
Basement membrane proteoglycan in various tissues: characterization using monoclonal antibodies to the Engelbreth-Holm-Swarm mouse tumor low density heparan sulfate proteoglycan. (1988)

The Engelbreth-Holm-Swarm mouse tumor has been found to produce at least two molecular species of heparan sulfate proteoglycan, a low density one (LD) and a high density one, which differ not only in core proteins but also in glycosaminoglycan structures (Kato, M., Y. Koike, Y. Ito, S. Suzuki, and K. Kimata. 1987. J. Biol. Chem. 262:7180-7188). With aim at investigating their distribution and possible functions in tissues, monoclonal antibodies were produced. Hybridomas obtained by fusion of NS-1 mouse myeloma cells with spleen cells from the rat immunized with a mixture of these proteoglycans were selected by their ability to react with the antigen. Two of them secreted monoclonal antibodies (IgG2a), designated HK-84 and HK-102, that recognize specifically the core protein moiety of LD. Immunofluorescent staining of various tissues (skeletal muscle, cardiac muscle, lung, brain, and kidney) with these monoclonal antibodies has demonstrated that the antigen molecules were present in all basement membranes of these tissues. SDS-PAGE of heparitinase-treated proteoglycan fractions prepared from these tissues and subsequent immunoblotting using these monoclonal antibodies have confirmed that the antigen molecule was LD, and further suggested that there was a tissue-specific variation in the core molecular size. Based on these results, we propose that LD may be an essential component in all basement membranes.
Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody (2020)

ABSTRACT The newly identified 2019 novel coronavirus (2019-nCoV) has caused more than 800 laboratory-confirmed human infections, including 25 deaths, posing a serious threat to human health. Currently, however, there is no specific antiviral treatment or vaccine. Considering the relatively high identity of receptor binding domain (RBD) in 2019-nCoV and SARS-CoV, it is urgent to assess the cross-reactivity of anti-SARS-CoV antibodies with 2019-nCoV spike protein, which could have important implications for rapid development of vaccines and therapeutic antibodies against 2019-nCoV. Here, we report for the first time that a SARS-CoV-specific human monoclonal antibody, CR3022, could bind potently with 2019-nCoV RBD (KD of 6.3 nM). The epitope of CR3022 does not overlap with the ACE2 binding site within 2019-nCoV RBD. Therefore, CR3022 has the potential to be developed as candidate therapeutics, alone or in combination with other neutralizing antibodies, for the prevention and treatment of 2019-nCoV infections. Interestingly, some of the most potent SARS-CoV-specific neutralizing antibodies (e.g., m396, CR3014) that target the ACE2 binding site of SARS-CoV failed to bind 2019-nCoV spike protein, indicating that the difference in the RBD of SARS-CoV and 2019-nCoV has a critical impact for the cross-reactivity of neutralizing antibodies, and that it is still necessary to develop novel monoclonal antibodies that could bind specifically to 2019-nCoV RBD.
Influence of Type of Surgery on the Occurrence of Parasympathetic Reinnervation After Cardiac Transplantation (1998)

Parasympathetic reinnervation depends on the surgical technique: because bicaval surgery cuts all sympathetic and parasympathetic nerves, regeneration might be stimulated similarly in both branches. Standard surgery cuts only approximately 50% of sympathetic fibers; most recipient parasympathetic axons remain intact, hence their regeneration might not be stimulated.
Isoform-Specific Functions of Phosphoinositide 3-Kinases: p110δ but Not p110γ Promotes Optimal Allergic Responses In Vivo (2008)

The leukocyte-enriched p110gamma and p110delta isoforms of PI3K have been shown to control in vitro degranulation of mast cells induced by cross-linking of the high affinity receptor of IgE (FcepsilonRI). However, the relative contribution of these PI3K isoforms in IgE-dependent allergic responses in vivo is controversial. A side-by-side comparative analysis of the role of p110gamma and p110delta in mast cell function, using genetic approaches and newly developed isoform-selective pharmacologic inhibitors, confirms that both PI3K isoforms play an important role in FcepsilonRI-activated mast cell degranulation in vitro. In vivo, however, only p110delta was found to be required for optimal IgE/Ag-dependent hypersensitivity responses in mice. These observations identify p110delta as a key therapeutic target among PI3K isoforms for allergy- and mast cell-related diseases.
Effects of Pushing Agents on the Separation and Detection of Debranched Amylopectin by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (1995)

Abstract The effects of acetate and nitrate as pushing agents on the high-performance anion-exchange chromatography with pulsed amperometric detector (HPAEC-PAD) for the separation and detection of debranched amylopectin were evaluated. Our experimental data indicated that nitrate could differentiate branched from linear isomers, whereas acetate could not. Therefore, the nitrate system had better separating power than the acetate system provided that the chromatograms under both gradient systems were completed within 100 min. Nitrate was more compatible with the detector than acetate; thus, a higher detector response resulted. Under our experimental conditions, with a signal-to-noise ratio of two, the number of DP (degree of polymerization) at various concentrations that was resolved by the nitrate system (62 at 0.5 mg/mL, 65 at 1.0 mg/mL, and 66 at 1.5 mg/mL) was larger than those resolved by the acetate system (56 at 0.5 mg/mL, 58 at 1.0 mg/mL, and 58 at 1.5 mg/mL). The chromatograms obtained from the nitrate gradient system were more reproducible than those from the acetate gradient system in terms of the consistencies of retention time and the peak area of the chromatographic peak. To various concentrations of the component with the same DP, the detector responses were more consistent with nitrate as a pushing agent compared with acetate. Neither pushing agent, however, resulted in a quantitative response for homologous debranched amylopectin.
A method for registration of 3-D shapes (1992)

The authors describe a general-purpose, representation-independent method for the accurate and computationally efficient registration of 3-D shapes including free-form curves and surfaces. The method handles the full six degrees of freedom and is based on the iterative closest point (ICP) algorithm, which requires only a procedure to find the closest point on a geometric entity to a given point. The ICP algorithm always converges monotonically to the nearest local minimum of a mean-square distance metric, and the rate of convergence is rapid during the first few iterations. Therefore, given an adequate set of initial rotations and translations for a particular class of objects with a certain level of 'shape complexity', one can globally minimize the mean-square distance metric over all six degrees of freedom by testing each initial registration. One important application of this method is to register sensed data from unfixtured rigid objects with an ideal geometric model, prior to shape inspection. Experimental results show the capabilities of the registration algorithm on point sets, curves, and surfaces.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Human activity detection in MPEG sequences (2002)

We propose a hierarchical method for human detection and activity recognition in MPEG sequences. The algorithm consists of three stages at different resolution levels. The first step is based on the principal component analysis of MPEG motion vectors of macroblocks grouped according to velocity, distance and human body proportions. This step reduces the complexity and amount of processing data. The DC DCT components of luminance and chrominance are the input for the second step, to be matched to activity templates and a human skin template. A more detailed analysis of the uncompressed regions extracted in previous steps is done at the last step via model-based segmentation and graph matching. This hierarchical scheme enables working at different levels, from low complexity to low false rates. It is important and interesting to realize that significant information can be obtained from the compressed domain in order to connect to high level semantics.
In vivo effects of murine hybridoma monoclonal antibody in a patient with T-cell leukemia (1981)

A murine monoclonal antibody directed against a normal T-cell differentiation antigen was given to a patient with adult T-cell leukemia. Immunofluorescence staining showed increased amounts of this antigen on the patient's leukemia cells. Using a competition radioimmunoassay, free antigen was not detectable in the serum prior to therapy. Two courses of in vivo therapy were given using a 1-mg dose. Each produced a prompt and dramatic fall in WBC with return to pretreatment levels over the ensuing 24 hr, a pattern similar to that seen with leukopheresis. After the first dose of antibody, circulating free antigen became detectable in the serum and a transient decline in creatinine clearance was noted. A 5-mg dose of antibody given at that time was ineffective, presumably because it was blocked by free antigen. Antigenic modulation by leukemia cells was found transiently following each course of antibody. A weak and clinically insignificant host antimouse antibody response was found 5 days after the first treatment. The patient tolerated antibody therapy without difficulty. Monoclonal antibodies offer promise as an immunotherapeutic approach to cancer but problems encountered here must be addressed.
Protein conjugates of synthetic saccharides elicit higher levels of serum IgG lipopolysaccharide antibodies in mice than do those of the O-specific polysaccharide fromShigella dysenteriaetype 1 (1999)

Our development of vaccines to prevent shigellosis is based on the hypothesis that a critical (protective) level of serum IgG to the O-specific polysaccharide (O-SP) domain of Shigella lipopolysaccharide (LPS) confers immunity. The O-SP is a hapten and must be conjugated to a protein to induce serum antibodies. The O-SP of Shigella dysenteriae type 1 (approximately 27 tetrasaccharide repeat units), prepared by acid hydrolysis of the LPS, was bound to human serum albumin (HSA) by multiple point attachment (O-SP-HSA): The molar ratio of HSA to O-SP was 1.0. Synthetic saccharides, composed of one or multiples of the O-SP tetrasaccharide, equipped with a spacer at their reducing end, were bound to HSA by a single point attachment: The average molar ratios of the saccharides to HSA ranged from 4 to 24. Serum IgG anti-LPS, elicited in mice by O-SP-HSA or synthetic tetra-, octa-, dodeca-, and hexadecasaccharide fragments, was measured by ELISA. Outbred 6-week-old female mice were injected s.c. three times at biweekly intervals with 2.5 micrograms of saccharide as a conjugate and were bled 7 days after the second and third injections. Excepting the tetramer, conjugates of the octamer, dodecamer and hexadecamer elicited IgG LPS antibodies after the second injection, a statistically significant rise (booster) after the third injection, and higher levels than those vaccinated with O-SP-HSA (P = 0.0001). The highest geometric mean levels of IgG anti-LPS were elicited by the hexadecamer with 9 chains or 9 moles of saccharide/HSA (15.5 ELISA units) followed by the octamer with 20 chains (11.1 ELISA units) and the dodecamer with 10 chains (9.52 ELISA units). Clinical evaluation of these synthetic saccharides bound to a medically useful carrier is planned.
Development of Vehicle On-board Communication System for Harsh Environment (2013)

Utilizing the already installed train interconnection cables as the physical layer of an extended intra-train communication could be a cost-effective solution. However these interconnection solutions are not optimal for the standardized digital data transfer solutions. The paper gives a brief summary of the theoretical aspects of data transmission, and experimental test results of the digital data transfer using non-standard physical layers.
Erratum: Parametric amplification in Josephson junction embedded transmission lines [Phys. Rev. B87, 144301 (2013)] (2013)

Received 20 September 2013DOI:https://doi.org/10.1103/PhysRevB.88.219904©2013 American Physical Society
An Anti-c-Fms Antibody Inhibits Orthodontic Tooth Movement (2008)

Orthodontic force induces osteoclastogenesis in vivo. It has recently been reported that administration of an antibody against the macrophage-colony-stimulating factor (M-CSF) receptor c-Fms blocks osteoclastogenesis and bone erosion induced by tumor necrosis factor-alpha (TNF-alpha) administration. This study aimed to examine the effect of an anti-c-Fms antibody on mechanical loading-induced osteoclastogenesis and osteolysis in an orthodontic tooth movement model in mice. Using TNF receptor 1- and 2-deficient mice, we showed that orthodontic tooth movement was mediated by TNF-alpha. We injected anti-c-Fms antibody daily into a local site, for 12 days, during mechanical loading. The anti-c-Fms antibody significantly inhibited orthodontic tooth movement, markedly reduced the number of osteoclasts in vivo, and inhibited TNF-alpha-induced osteoclastogenesis in vitro. These findings suggest that M-CSF plays an important role in mechanical loading-induced osteoclastogenesis and bone resorption during orthodontic tooth movement mediated by TNF-alpha.
Steric control of reactivity: formation of oximes, benzodiazepinone N-oxides and isoxazoloquinolinones (1998)

Reaction of the alkenyl carbonyl compounds 1 with hydroxylamine can lead to the formation of the oximes 2, the benzodiazepinone N-oxides 3 or the isoxazoloquinolinones 5. The product(s) of reaction are shown to depend on the electronic nature of the terminal olefinic substituent R3 and the space filling capacity of the substituents R1, R2 and R4. When the olefinic centre is electron poor (R3 = CO2Et) ketocarbonyls convert exclusively to bicyclic nitrones 3 whereas aldehydes are more sensitive to subtle changes in skeletal structure and give rise to oximes 2, tricycles 5 or mixtures of both. For aldehyde and ketone substrates when the olefinic centre carries an aryl substituent (R3 = Ph) the primary product of reaction is the corresponding oxime which on thermal activation converts to the tricyclic isoxazoloquinolinones.
Combining statistical and knowledge-based spoken language understanding in conditional models (2006)

Spoken Language Understanding (SLU) addresses the problem of extracting semantic meaning conveyed in an utterance. The traditional knowledge-based approach to this problem is very expensive --- it requires joint expertise in natural language processing and speech recognition, and best practices in language engineering for every new domain. On the other hand, a statistical learning approach needs a large amount of annotated data for model training, which is seldom available in practical applications outside of large research labs. A generative HMM/CFG composite model, which integrates easy-to-obtain domain knowledge into a data-driven statistical learning framework, has previously been introduced to reduce data requirement. The major contribution of this paper is the investigation of integrating prior knowledge and statistical learning in a conditional model framework. We also study and compare conditional random fields (CRFs) with perceptron learning for SLU. Experimental results show that the conditional models achieve more than 20% relative reduction in slot error rate over the HMM/CFG model, which had already achieved an SLU accuracy at the same level as the best results reported on the ATIS data.