gebhart/patrepeval huggingface dataset:
Near Infrared Reflectance Spectroscopy of Oil in Intact Canola Seed (Brassica Napus L.). II. Association between Principal Components and oil Content (2005)

This work reports that the measurements of the likeness or the uniqueness of the 1100–2500 nm reflectance spectra of intact canola seed determined from principal component analysis (PCA) approximated spectra with global H or neighbourhood H statistics were not associated with oil concentration within the seed. The absence of stability in association between the H measurements and oil content was related to inconsistency in the amount and distribution (between principal components) of the spectral variation correlated to the oil content within and between different batches of canola seed. PCA was used to approximate variation in the 1100–2500 nm, second order derivative, reflectance spectra of intact canola seed, acquired from 15 batches of seed samples. The first eight principal components (PCs) captured 97.14% to 99.35% of the total variance in the spectra. The amount of variation captured by individual components was independent of the number of samples in the batch and oil content within the seed. The pattern of variance distribution among principal components was inconsistent and highlighted the uniqueness of the origin of the spectral variation in each batch of canola seed. In this study, the strength of correlation between oil content and principal components was used as a measure of component significance to the analysis of oil in the intact canola seed. In the examined sets of spectra, oil content was correlated to the low order components, PC1 to PC4. In the 15 files of spectra, oil content showed the strongest correlation to PC2 in eight sets of data, to PC3 in four sets of data and to PC1 in three sets of data. The strength of association between oil and the individual components varied considerably in magnitude among examined files of spectra; r 2 = 0.28–0.81 for the first strongly correlated component, r 2 = 0.05–0.29 for the second and r 2 = 0.02–0.19 for the third. The position of the PCs in the correlation sequence was inconsistent and underlined differences of oil signal/spectral data interactions in the individual sets of data. Examination of principal component loadings showed that in the reported files of spectra, principal components correlated to the oil content frequently captured variance at segments, which denote absorptions specific and accidental to canola oil. The outline of the loadings did not conform to a single, regular pattern common to all sets of data. The reported results are in disagreement with rationale of the methodology, which involves the spectra matching techniques for validating the predictive efficiency of near infrared (NIR) calibrations. The reported results highlighted that the reliable NIR quantification of oil content from reflectance spectra of intact canola seed would require an independent validation for every acquired set of spectra.
Oscillator phase noise: a tutorial (2000)

Linear time-invariant (LTI) phase noise theories provide important qualitative design insights but are limited in their quantitative predictive power. Part of the difficulty is that device noise undergoes multiple frequency translations to become oscillator phase noise. A quantitative understanding of this process requires abandoning the principle of time invariance assumed in most older theories of phase noise. Fortunately, the noise-to-phase transfer function of oscillators is still linear, despite the existence of the nonlinearities necessary for amplitude stabilization. In addition to providing a quantitative reconciliation between theory and measurement, the time-varying phase noise model presented in this tutorial identifies the importance of symmetry in suppressing the upconversion of 1/f noise into close-in phase noise, and provides an explicit appreciation of cyclostationary effects and AM-PM conversion. These insights allow a reinterpretation of why the Colpitts oscillator exhibits good performance, and suggest new oscillator topologies. Tuned LC and ring oscillator circuit examples are presented to reinforce the theoretical considerations developed. Simulation issues and the accommodation of amplitude noise are considered in appendixes.
Novel applications of cryptography in digital communications (1990)

A tutorial on digital signatures is presented, covering the basic concepts of public-key cryptography and digital signatures based on public-key techniques. Applications are discussed, including key management for conventional encryption equipment, electronic mail and data interchange, access control and audit trails, software verification and virus detection, counterfeit-proof currency, nuclear test ban treaty detectors, and challenge response systems such as in aircraft identification of friend or foe.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Sustained-release progesterone nanosuspension following intramuscular injection in ovariectomized rats (2010)

The production of an intramuscular (IM) injection of natural progesterone would provide a safer solution than using semi synthetic progesterone. However, disadvantages such as low solubility and a short half life prevent the use of natural progesterone. In this study, we formulated a sustained release form of natural progesterone to be given as IM injection. A progesterone nanosuspension (PNS) was first developed and then dispersed in a thermosensitive gel matrix. The selected nanoparticles showed an average particle size of 267 nm and a zeta potential approaching-41 mV. The in vitro release profile of PNS from the F127 plus methyl cellulose gel followed zero order kinetics and correlated linearly with the weight percentage of gel dissolved, demonstrating that the overall rate of release of PNS is controlled by dissolution of the pluronic F127/methyl cellulose (MC) gel (r² > 0.99). The pharmacokinetic parameters of the PNS (6 mg/mL) in pluronic F127/MC gel were evaluated in comparison with the control progesterone suspension. After the administration of PNS in F127/MC gel into the rats, a maximum serum concentration of 22.1 ± 1.9 ng/mL was reached at a T(max) of 4.05 ± 0.1 h. The terminal half life was 12.7 ± 0.8 h. The area under the curve AUC₀₋∞ of the injected formula was 452.75 ± 42.8 ng·h/mL and the total mean residence time was 18.57 ± 1.44 h. The PNS in gel was significantly different from the control in rate and extent at P < 0.001. The natural progesterone which was nanosized and formulated in a thermosensitive gel significantly sustained the action of natural progesterone so that it could be injected every 36 h instead of every day. Moreover, this formula is expected to provide a much safer choice than the use of semi-synthetic progesterone.
Electrochemical Etching of Silicon by Hydrazine (1993)

The anodic dissolution and passivation of n- and p-type Si in different concentrations of hydrazine solution and etching temperatures were studied. During etching, performed at 70 and 90°C, it was observed that the current-potential characteristics for both n-and p-type Si showed a current reduction after reaching a peak value. A linear 1-V relation was observed for the room temperature etching. A mechanism, which accounts for the semiconductor energy level change in solution as under different biasing conditions, is proposed to give a qualitative explanation of the different 1-V behaviors for n-and p-type semiconductors. © 1993, The Electrochemical Society, Inc. All rights reserved.
A hierarchical quorum‐sensing cascade in Pseudomonas aeruginosa links the transcriptional activators LasR and RhIR (VsmR) to expression of the stationary‐phase sigma factor RpoS (1996)

In Pseudomonas aeruginosa, the production of many virulence factors and secondary metabolites is regulated in concert with cell density through quorum sensing. Two quorum-sensing regulons have been identified in which the LuxR homologues LasR and RhlR are activated by N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) and N-butanoyl-L-homoserine lactone (BHL) respectively. The lasR and rhlR genes are linked to the luxl homologues lasl and rhll, which are responsible for synthesis of OdDHL and BHL, respectively. As lasRl and rhlRl are both involved in regulating synthesis of exoenzymes such as elastase, we sought to determine the nature of their interrelationship. By using lacZ transcriptional fusions in both homologous (P. aeruginosa) and heterologous (Escherichia coli) genetic backgrounds we provide evidence that (i) lasR is expressed constitutively throughout the growth cycle, (ii) rhlR expression is regulated by LasR/OdDHL, and (iii) that RhlR/BHL regulates rhll. We also show that expression of the stationary-phase sigma factor gene rpoS is abolished in a P. aeruginosa lasR mutant and in the pleiotropic BHL-negative mutant PANO67. Furthermore, our data reveal that kin E. coli, an rpoS-lacZ fusion is regulated directly by RhlR/BHL. Taken together, these results indicate that P. aeruginosa employs a multilayered hierarchical quorum-sensing cascade involving RhlR/BHL and LasR/OdDHL, interlinked via RpoS, to integrate the regulation of virulence determinants and secondary metabolites with adaptation and survival in the stationary phase.
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery (2014)

The CRISPR/Cas9 system is a robust genome editing technology that works in human cells, animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 enzyme. Building on previous work (Jinek et al., 2013), we show here that new genetic information can be introduced site-specifically and with high efficiency by homology-directed repair (HDR) of Cas9-induced site-specific double-strand DNA breaks using timed delivery of Cas9-guide RNA ribonucleoprotein (RNP) complexes. Cas9 RNP-mediated HDR in HEK293T, human primary neonatal fibroblast and human embryonic stem cells was increased dramatically relative to experiments in unsynchronized cells, with rates of HDR up to 38% observed in HEK293T cells. Sequencing of on- and potential off-target sites showed that editing occurred with high fidelity, while cell mortality was minimized. This approach provides a simple and highly effective strategy for enhancing site-specific genome engineering in both transformed and primary human cells.
Apoptosis Signaling (2000)

Apoptosis, a physiological process for killing cells, is critical for the normal development and function of multicellular organisms. Abnormalities in cell death control can contribute to a variety of diseases, including cancer, autoimmunity, and degenerative disorders. Signaling for apoptosis occurs through multiple independent pathways that are initiated either from triggering events within the cell or from outside the cell, for instance, by ligation of death receptors. All apoptosis signaling pathways converge on a common machinery of cell destruction that is activated by a family of cysteine proteases (caspases) that cleave proteins at aspartate residues. Dismantling and removal of doomed cells is accomplished by proteolysis of vital cellular constituents, DNA degradation, and phagocytosis by neighboring cells. This article reviews current knowledge of apoptosis signaling, lists several pressing questions, and presents a novel model to explain the biochemical and functional interactions between components of the cell death regulatory machinery.
Electrical transport properties of single ZnO nanorods (2004)

Single ZnO nanorods with diameters of ∼130nm were grown on Au-coated Al2O3 substrates by catalyst-driven molecular beam epitaxy. Individual nanorods were removed from the substrate and placed between Ohmic contact pads and the current–voltage characteristics measured as a function of temperature and gas ambient. In the temperature range from 25to150°C, the resistivity of nanorods treated in H2 at 400°C prior to measurement showed an activation energy of 0.089±0.02eV and was insensitive to the ambient used (C2H4,N2O,O2 or 10% H2 in N2). By sharp contrast, the conductivity of nanorods not treated in H2 was sensitive to trace concentrations of gases in the measurement ambient even at room temperature, demonstrating their potential as gas sensors.
Kv7 (KCNQ) Channel Modulators and Neuropathic Pain (2007)

ADVERTISEMENT RETURN TO ISSUEPREVPerspectiveNEXTKv7 (KCNQ) Channel Modulators and Neuropathic PainGordon Munro and William Dalby-BrownView Author Information NeuroSearch A/S, Pederstrupvej 93, DK-2750 Ballerup, Denmark Cite this: J. Med. Chem. 2007, 50, 11, 2576–2582Publication Date (Web):May 10, 2007Publication History Received16 August 2006Published online10 May 2007Published inissue 1 May 2007https://doi.org/10.1021/jm060989lCopyright © 2007 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views1793Altmetric-Citations47LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (101 KB) Get e-AlertsSUBJECTS:Modulators,Neurophysiology,Pharmaceuticals,Reaction products,Rodent models Get e-Alerts
Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. (1990)

We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to study biochemical events in the transport of macromolecules across the nuclear envelope. While treatment of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes remain structurally intact and nuclei retain the ability to transport and accumulate proteins containing the SV40 large T antigen nuclear location sequence. Transport requires addition of exogenous cytosol to permeabilized cells, indicating the soluble cytoplasmic factor(s) required for nuclear import are released during digitonin treatment. In this reconstituted import system, a protein containing a nuclear location signal is rapidly accumulated in nuclei, where it reaches a 30-fold concentration compared to the surrounding medium within 30 min. Nuclear import is specific for a functional nuclear location sequence, requires ATP and cytosol, and is temperature dependent. Furthermore, accumulation of the transport substrate within nuclei is completely inhibited by wheat germ agglutinin, which binds to nuclear pore complexes and inhibits transport in vivo. Together, these results indicate that the permeabilized cell system reproduces authentic nuclear protein import. In a preliminary biochemical dissection of the system, we observe that the sulfhydryl alkylating reagent N-ethylmaleimide inactivates both cytosolic factor(s) and also component(s) in the insoluble permeabilized cell fraction required for nuclear protein import. Because this permeabilized cell model is simple, efficient, and works effectively with cells and cytosol fractions prepared from a variety of different vertebrate sources, it will prove powerful for investigating the biochemical pathway of nuclear transport.
Hard shell gas-filled contrast enhancement particles for colour Doppler ultrasound imaging of tumors (2010)

Hollow hard shell particles of 200 nm and 2 micron diameter with a 10 nm thick porous silica shell have been synthesized using polystyrene templates and a sol-gel process. The template ensures than the hollow particles are monodispersed, while the charged silica surface ensures that they remain suspended in solution for weeks. When filled with perfluorocarbon gas, the particles behave as an efficient contrast agent for colour Doppler ultrasound imaging in human breast tissue. The silica shell provides unique properties compared to conventional soft shell particles employed as ultrasound contrast agents: uniform size control, strong adsorption to tissue and cells immobilizing particles at the tissue injection site, a long imaging lifetime, and a silica surface that can be easily modified with biotargeting ligands or small molecules to adjust the surface charge and polarity.
Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR (1999)

The self-assembly of bis-biotinylated double-stranded DNA and the tetravalent biotin-binding protein streptavidin (STV) have been studied by non-denaturing gel electrophoresis and atomic force microscopy (AFM). The rapid self-assembly reproducibly generated populations of individual oligomeric complexes. Most strikingly, the oligomers predominantly contained bivalent STV molecules bridging two adjacent DNA fragments to form linear nanostructures. Trivalent STV branch points occurred with a lower frequency and the presence of tetravalent STV was scarce. However, valency distribution, size and the exchange dynamics of the supramolecular aggregates were highly sensitive to stoichiometric variations in the relative molar coupling ratio of bis-biotinylated DNA and STV. The largest aggregates were obtained from equimolar amounts while excess STV led to the formation of smaller oligomers appearing as fingerprint-like band patterns in electrophoresis. Excess DNA, however, induces a complete breakdown of the oligomers, likely a consequence of the instability of STV conjugates containing more than two biotinylated DNA fragments. It was demonstrated that the oligomers can further be functionalized, for instance by the coupling of biotinylated immunoglobulins. Both pure and also antibody-modified DNA-STV oligomers were used as reagents in immuno-PCR (IPCR), a highly sensitive detection method for proteins and other antigens. Employment of the supramolecular reagents led to an approximately 100-fold enhanced sensitivity compared to the conventional IPCR procedure.
Multiplex single-base extension typing to identify nuclear genes required for RNA editing in plant organelles (2008)

We developed a multiplex single-base extension single-nucleotide polymorphism-typing procedure for screening large numbers of plants for mutations in mitochondrial RNA editing. The high sensitivity of the approach detects changes in the RNA editing status generated in total cellular cDNA from pooled RNA preparations of up to 50 green plants. The method has been employed to tag several nuclear encoded genes required for RNA editing at specific sites in mitochondria of Arabidopsis thaliana. This approach will allow large-scale screening for mutations in genes encoding trans-factors for many types of RNA editing as well as for other RNA modifications.
Probing the structure‐activity relationship of Escherichia coli LT‐A by site‐directed mutagenesis (1994)

Computer analysis of the crystallographic structure of the A subunit of Escherichia coli heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully assembled, non-toxic new molecules containing mutations of single amino acids such as Val-53-->Glu or Asp, Ser-63-->Lys, Val-97-->Lys, Tyr-104-->Lys or Asp, and Ser-114-->Lys or Glu. This group also included mutations in amino acids such as Arg-7, Glu-110 and Glu-112 that were already known to be important for enzymatic activity. The second group was formed by mutations that caused the collapse or prevented the assembly of the A subunit: Leu-41-->Phe, Ala-45-->Tyr or Glu, Val-53-->Tyr, Val-60-->Gly, Ser-68-->Pro, His-70-->Pro, Val-97-->Tyr and Ser-114-->Tyr. The third group contained those molecules that maintained a wild-type level of toxicity in spite of the mutations introduced: Arg-54-->Lys or Ala, Tyr-59-->Met, Ser-68-->Lys, Ala-72-->Arg, His or Asp and Arg-192-->Asn. The results provide a further understanding of the structure-function of the active site and new, non-toxic mutants that may be useful for the development of vaccines against diarrhoeal diseases.
Interferon activation and innate immunity. (2000)

The interferons are a family of cytokine mediators critically involved in alerting the cellular immune system to viral infection of host cells. Interferons not only exhibit important antiviral effects but also exert a key influence on the quality of the cellular immune responses and amplify antigen presentation to specific T cells. Type I interferon (IFN-alpha and IFN-beta) is secreted by virus-infected cells while type II, immune or gamma interferon (IFN-gamma) is mainly secreted by T cells, natural killer (NK) cells and macrophages. Interferons interact with specific cellular receptors, which promote production of second messengers ultimately leading to expression of antiviral and immune modulatory genes. The IFN genes themselves are regulated by transcriptional and posttranscriptional mechanisms including modulation by a family of interferon regulatory factors (IRFs) synthesised by host cells. IFNs activate macrophages, induce B cells to switch immunoglobulin type, alter T helper response, inhibit cell growth, promote apoptosis and induce an antiviral state in uninfected cells. The therapeutic potential of the IFNs is currently the focus of intense attention in a number of virus-associated diseases, tumours and autoimmune disorders.
<title>Performance of a 4096-pixel photon counting chip</title> (1998)

A 4096 pixel Photon Counting Chip (PCC) has been developed and tested. It is aimed primarily at medical imaging although it can be used for other applications involving particle counting. The readout chip consists of a matrix of 64 x 64 identical square pixels, whose side measures 170 mm and is bump-bonded to a similar matrix of GaAs or Si pixel diodes covering a sensitive area of 1.18 cm . The electronics in each cell comprises a preamplifier, a discriminator with variable threshold and a 3-bit threshold tune as well as 15-bit counter. Each pixel can be individually addressed for electrical test or masked during acquisition. A shutter allows for switching between the counting and the readout modes and the use of a static logic in the counter enables long data taking periods. Electrical tests of the chip have shown a maximum counting rate of up to 2 MHz in each pixel. The minimum reachable threshold is 1400 e with a variation of 350 e rms that can be reduced to 80 e rms after tuning with the 3-bit adjustment. Electical noise at the input is 170 e rms. Several read-out chips have been bump-bonded to 200 mm thick GaAs detectors. Tests with g-rays and b sources have been carried out. A number of objects have been imaged and 260 mm thick aluminium foil which represents a contrast to the surrounding aire of only 1.9% has been correctly imaged.
Hypertriglyceridemia and Its Pharmacologic Treatment Among US Adults (2009)

Among US adults, hypertriglyceridemia is common. Until the benefits of treating hypertriglyceridemia that is not characterized by extreme elevations of TG concentration with medications are incontrovertible, therapeutic lifestyle change remains the preferred treatment.