gebhart/patrepeval huggingface dataset:
From Chance to Choice in the Generation of Neural Cell Lines (1991)

Despite the central importance of cell lines in contemporary studies in cellular and molecular biology, many areas of potential investigation remain impeded by the limited number of lines available and by the difficulty in generating new lines of interest. Thus, there has been a constant pressure to develop improved methods for obtaining cell lines of particular interest. This review examines some of the problems associated with in vitro approaches to cell line generation. In addition, two different ways in which transgenic animals can be used to overcome the limitations of in vitro production of cell lines are discussed. In the first approach, specific promoters are utilized to target expression of immortalizing genes to cells of interest. The second approach is concerned with development of a strain of transgenic animals (the H-2KbtsA58 transgenic mouse) designed to obviate the need for identification of cell-type specific promoters, and in which it is theoretically possible to directly generate conditionally immortal cell lines from any tissue of the body by simple dissection and growth of cells in appropriate tissue culture conditions. Finally, approaches are also discussed in which investigations on the control of precursor differentiation have been applied so as to bypass the need for expression of activated immortalizing oncogenes in the generation of large quantities of conditionally immortalized cells with the capacity to undergo normal differentiation in vitro and in vivo.
Dietary Administration of High Doses of Pterostilbene and Quercetin to Mice Is Not Toxic (2009)

The aim of this study is to evaluate possible harmful effects of high doses of t-pterostilbene (t-PTER) and quercetin (QUER) in Swiss mice. Mice were fed during 28 days at doses of 0, 30, 300, and 3000 mg/kg body weight/day of t-PTER, QUER, or a mixture of both, t-PTER + QUER, which are equivalent to 5, 50, and 500 times, respectively, the estimated mean human intake of these polyphenols (25 mg/day). Daily oral administration of QUER, t-PTER, or a mixture of both of them did not cause mortality during the experimental period. There were no differences in food and water consumption on sex. No significant body weight gain in the male or female groups was observed. Red blood cell number and the hematocrit increased after polyphenols administration compared to control groups. Biochemical parameters were not affected. Histopathological examination revealed no alterations in clinical signs or organ weight at any dose.
Epidemiology of cervical cancer. (1989)

Cervical cancer is on the rise worldwide and is estimated to comprise of all cancers the number of new cases of cervical cancer per year is around 465000. 20% of these cases occur in developed countries with 80% in developing countries. Cervical cancer presently ranks 5th; 7.3% of all human cancers worldwide. In Africa Central and South America Asia and outside Japan cervical cancer is the leading cancer among women and constitutes 20-30% of all cancers in women. Contrasting figures exist for North America Australia Northern and Western Europe with cervical cancer accounting for 4-6% of all female cancers. Estimates are crude because of poor assessment of population and incidences of cervical cancer in certain areas of the world. With larger populated areas differences are not as easily detected between small geographical unites such as regions countries or population groups classified by ethnicity religious origin or residential status. Epidemiological evidence indicates that number of womens sexual partners age of first intercourse smoking long-term oral contraceptive use dietary factors and immunosuppressants are the principal determinants of womens cervical cancer risk. How each of these determinants contribute to cervical cancer cannot be adequately assessed with data. There is some evidence of an association between cervical cancer and various infectious agents including syphilis herpes simplex virus 2 and chlamydia trachomatis.
Relation-Shape Convolutional Neural Network for Point Cloud Analysis (2019)

Point cloud analysis is very challenging, as the shape implied in irregular points is difficult to capture. In this paper, we propose RS-CNN, namely, Relation-Shape Convolutional Neural Network, which extends regular grid CNN to irregular configuration for point cloud analysis. The key to RS-CNN is learning from relation, i.e., the geometric topology constraint among points. Specifically, the convolutional weight for local point set is forced to learn a high-level relation expression from predefined geometric priors, between a sampled point from this point set and the others. In this way, an inductive local representation with explicit reasoning about the spatial layout of points can be obtained, which leads to much shape awareness and robustness. With this convolution as a basic operator, RS-CNN, a hierarchical architecture can be developed to achieve contextual shape-aware learning for point cloud analysis. Extensive experiments on challenging benchmarks across three tasks verify RS-CNN achieves the state of the arts.
Microfluidic electrowetting-based droplet mixing (2002)

We present a liquid droplet mixer to enable mixing of the samples and reagents for chemical and biological analysis in micro total analysis systems (/spl mu/TAS). The droplets in the mixer are actuated based on electrowetting phenomenon. The actuator comprises of two parallel glass plates between which the droplet is actuated on a planar array of electrodes. Mixing of liquid channels in microfluidic systems has been demonstrated for continuous flow systems where the mixing is diffusion-limited due to the laminar flow of liquids, requiring very long, thin channels. In the present paper, mixing is performed on discrete droplets of liquid. When two droplets are brought together, depending on the velocity of the moving droplets, surface tension, viscosity, electrode activation, and volume among other factors, turbulence is created which aids in mixing. The mixing is not limited by diffusion and enhanced by transport. The mixing experiments are performed between fluorescein and plain water droplets whose individual volume is 1.75 /spl mu/l. Mixing is visualized with a 2-CCD camera setup to observe both the top and side views, with appropriate filters to capture fluorescence. We observed that it takes about 60 seconds for two droplets of 1.75 /spl mu/l each to mix when their surface tensions are different and it takes about 90 seconds when the two droplets have similar surface tensions. The present mixer stands apart from any current conventional micromixers in that the mixing times occupies much lesser area, mixing does not need any specific architecture on the chip and can be performed on any transport electrodes dynamically assigned to mixing.
A Fully Passive Wireless Microsystem for Recording of Neuropotentials Using RF Backscattering Methods (2011)

The ability to safely monitor neuropotentials is essential in establishing methods to study the brain. Current research focuses on the wireless telemetry aspect of implantable sensors in order to make these devices ubiquitous and safe. Chronic implants necessitate superior reliability and durability of the integrated electronics. The power consumption of implanted electronics must also be limited to within several milliwatts to microwatts to minimize heat trauma in the human body. In order to address these severe requirements, we developed an entirely passive and wireless microsystem for recording neuropotentials. An external interrogator supplies a fundamental microwave carrier to the microsystem. The microsystem comprises varactors that perform nonlinear mixing of neuropotential and fundamental carrier signals. The varactors generate third-order mixing products that are wirelessly backscattered to the external interrogator where the original neuropotential signals are recovered. Performance of the neuro-recording microsystem was demonstrated by wireless recording of emulated and in vivo neuropotentials. The obtained results were wireless recovery of neuropotentials as low as approximately 500 microvolts peak-to-peak (μV(pp)) with a bandwidth of 10 Hz to 3 kHz (for emulated signals) and with 128 epoch signal averaging of repetitive signals (for in vivo signals).
Evolution of human immunoglobulin kappa J region genes. (1982)

Immunoglobulin kappa chain variable region genes are assembled from two discontinuous DNA segments, a V and a J gene. The J region genes, in addition to encoding amino acid positions 96-108 of the kappa polypeptide chain, also provide sequences required for both DNA and RNA splicing reactions. For purposes of evolutionary comparison and to establish the complexity of the kappa J region locus in man, we have determined an approximately 3000 basepair nucleotide sequence in a cloned human DNA fragment that encodes the germline distinct J region segments. Significant blocks of homology have been tightly maintained between this region and an analogous segment of the mouse genome. In particular, the short sequences, GGTTTTTGT and CACTGTG, thought to be involved in V-J recombination, are the most highly conserved regions (97% homology). In addition, from heteroduplex data and computer analysis of the nucleotide sequences, it is clear that the mouse J3 sequence, a pseudogene, is not present in the human cluster. This can be explained by a duplication event in the mouse J region gene cluster that may have been the result of unequal crossing over between homologous chromosomes.
Genetic Contribution of the Interleukin‐10 Promoter Polymorphism in Endometriosis Susceptibility (2002)

IL-10 promoter polymorphisms were associated with the production of anti-CA II ab in patients with endometriosis, suggesting a role in the genetic susceptibility for endometriosis.
Relationship between active oxygen damage and tonoplast H~(+)-ATPase activity in leaves of barley seedling under salt stress (1998)

In leaves of barley seedlings, 300mmol/L NaCl stress resulted in the increase of the amount of Na~(+), superoxide radical, hydrogen peroxide, malondialdehyde(MDA), and electrical conductivity. It also caused the decrease of the amount of K~(+), chlorophyll(Chl), K~(+)/Na~(+) ratio and tonoplast H~(+)-ATPase activity. Chl content was negatively related to H_(2)O_(2), O~(.)_(2) and MDA at significant level, so was electrical conductivity. Under salt stress, the decrease of tonoplast H~(+)-ATPase activity was accompanied by the increase of contents of H_(2)O_(2) and O~(.)_(2). The results showed that Chl degration and tonoplast functional damage in barley seedings induced by salt stress may be mainly due to the accumulation of active oxygen.
Capabilities and limitations of ion mobility spectrometry for field screening applications (1997)

The objective of this review is to provide an overview of the capabilities and limitations of ion mobility spectrometry (IMS) as it pertains to field screening. The first section of the review provides a description of the instrument and its operation along with examples of its primary advantages of simplicity, selectivity, and sensitivity. IMS is a simple and potentially inexpensive analytical technique with tunably variable selectivity of response, especially for polar organic compounds, and with sensitivity on the order of 0.1 ppb for most vapor-phase compounds. The second section describes the history of IMS as a field-portable technique and discusses some of the difficulties experienced in this capacity. Problems in IMS include competitive ion/molecule reactions, low resolution, a limited dynamic response range, ease of contamination, long residence times in the spectrometer, and concentration-dependent response characteristics. Some of these problems have been solved, while others await further developments in IMS before they cease to limit the utility of IMS in reliable field-portable instruments. Finally, future developments which are needed in order for IMS to reach its full capacity in portable instruments for field monitoring are discussed. These future developments include nontime-of-flight instruments, high-temperature operation, and nonradioactive ionization sources. © 1997 John Wiley & Sons, Inc. Field Analyt Chem Technol 1: 119–134, 1997
Activating Fc Receptors Are Required for Antitumor Efficacy of the Antibodies Directed toward CD25 in a Murine Model of Adult T-Cell Leukemia (2004)

We previously showed therapeutic efficacy of humanized anti-Tac (HAT), murine anti-Tac (MAT), and 7G7/B6 monoclonal antibodies, which recognize CD25, for human adult T-cell leukemia (ATL) in a murine model. In this study, we investigated the mechanism underlying the tumor-killing action mediated by these antibodies on an ATL model in nonobese diabetic/severe combined immunodeficient (SCID/NOD) wild-type mice that lack effective T and natural killer (NK) cells and in SCID/NOD Fc receptor common gamma chain knockout (FcRgamma-/-) mice. The ATL model was established by i.p. injection of human ATL cells (MET-1) into SCID/NOD wild-type or SCID/NOD FcRgamma-/- mice. HAT, MAT, and 7G7/B6 were given to the leukemia-bearing mice at a dose of 100 microg weekly for 4 weeks. The three antibodies inhibited the leukemia growth significantly in SCID/NOD wild-type mice, as monitored by serum levels of human beta2-microglobulin (P < 0.01), and prolonged survival of the leukemia-bearing SCID/NOD wild-type mice (P < 0.01) as compared with the control group. However, none of the antibodies manifested efficacy on the leukemia growth and survival of the SCID/NOD FcRgamma-/- mice bearing MET-1 leukemia. In a pharmacokinetics study, the blood concentrations of the radiolabeled antibodies decreased with time similarly in SCID/NOD wild-type and SCID/NOD FcRgamma-/- mice. Although NK cells may play a role in humans, in this murine model FcRgamma receptors on non-NK cells, such as polymorphonuclear leukocytes or monocytes, are required for the tumor-killing action of the antibodies directed toward CD25.
Present Status of Cerium(IV)-Cerium(III) Potentials (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPresent Status of Cerium(IV)-Cerium(III) PotentialsEarl. Wadsworth, F. R. Duke, and C. A. GoetzCite this: Anal. Chem. 1957, 29, 12, 1824–1825Publication Date (Print):December 1, 1957Publication History Published online1 May 2002Published inissue 1 December 1957https://pubs.acs.org/doi/10.1021/ac60132a046https://doi.org/10.1021/ac60132a046research-articleACS PublicationsRequest reuse permissionsArticle Views813Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Photofield-Effect in Amorphous InGaZnO TFTs (2008)

IMID/IDMC/ASIA DISPLAY 2008 DIGEST • Abstract We study the amorphous In-Ga-Zn-O thin-film transistors (TFTs) properties under monochromatic illumination (λ=420nm) with different intensity. TFT off-state drain current (IDS_off) was found to increase with the light intensity while field effect mobility (μeff) is almost unchanged; only small change was observed for sub-threshold swing (S). Due to photo-generated charge trapping, a negative threshold voltage (Vth) shift is also observed. The photofield-effect analysis suggests a highly efficient UV photocurrent conversion in a-IGZO TFT. Finally, a-IGZO mid-gap density-of-states (DOS) was extracted and is more than an order lower than reported value for a-Si:H, which can explain a good switching properties of the a-IGZO TFTs.
Monoclonal antibodies to lipocortin‐1 as probes for biological function (1990)

We have developed two monoclonal antibodies to human lipocortin-1 (103 and 105) as reagents for quantitating the protein in biological systems and neutralizing its activity. Lipo 105 is a high affinity antibody that is functional in ELISA and Western blot formats. The antibody recognizes a site between amino acids 30 and 55 in the lipocortin-1 sequence and can be used on native or denatured protein. Lipo 103 is an antibody that neutralizes the phospholipase A2 inhibitory activity of lipocortin-1 by blocking binding of the protein to phospholipid surfaces. The antibody is specific for native human lipocortin-1. Lipo 103 was recently shown to block lipocortin-1-dependent differentiation of a squamous carcinoma cell line, demonstrating its usefulness as a probe for function.
A region-based video coding method for remote monitoring of neurosurgery (2005)

Transmitting high-quality neurophysiology video via the Internet is a challenging problem in telemedicine. We propose a novel content-selective video data processing and compression method based on the discrete wavelet transform (DWT). Our algorithm is adaptive to intraoperative monitoring video data and has a great scalability on real-time network bandwidth allocation. When compared with the general-purpose video compression methods, our method offers higher quality within the critical field of neurosurgical video.
Spatial distribution of the DF508 mutation in cystic fibrosis: a review. (1992)

The recent finding of the most common mutation (DF508) in cystic fibrosis in white populations has led to the publication of numerous data regarding its distribution and frequency. A review of the available data shows that there is a gradient in both the incidence of cystic fibrosis and the frequency of the DF508 mutation in Europe, the highest values being found in western Europe. It is postulated that the DF508 mutation was present in a western European population before the arrival of the Indo-Europeans from the Middle East; the mutation then spread into these migrating populations.
Hematopoietic Stem Cell Cultures and Assays (2014)

The adult hematopoietic system is repopulated in its entirety from a rare cell type known as hematopoietic stem cells (HSCs) that reside in the marrow space throughout the skeletal system. Here we describe the isolation and identification of HSCs both phenotypically and functionally.
Polarity and cell fate specification in the control of Caenorhabditis elegans gastrulation (2009)

Gastrulation is a time during development when cells destined to produce internal tissues and organs move from the surface of the embryo into the interior. It is critical that the cell movements of gastrulation be precisely controlled, and coordinated with cell specification, in order for the embryo to develop normally. Caenorhabditis elegans gastrulation is relatively simple, can be observed easily in the transparent embryo, and can be manipulated genetically to uncover important regulatory mechanisms. Many of these cellular and molecular mechanisms, including cell shape, cytoskeletal, and cell cycle changes, appear to be conserved from flies to vertebrates. Here we review gastrulation in C. elegans, with an emphasis on recent data linking contact-induced cell polarity, PAR proteins, and cell fate specification to gastrulation control.
Eccentric Exercise Facilitates Mesenchymal Stem Cell Appearance in Skeletal Muscle (2012)

Eccentric, or lengthening, contractions result in injury and subsequently stimulate the activation and proliferation of satellite stem cells which are important for skeletal muscle regeneration. The discovery of alternative myogenic progenitors in skeletal muscle raises the question as to whether stem cells other than satellite cells accumulate in muscle in response to exercise and contribute to post-exercise repair and/or growth. In this study, stem cell antigen-1 (Sca-1) positive, non-hematopoetic (CD45⁻) cells were evaluated in wild type (WT) and α7 integrin transgenic (α7Tg) mouse muscle, which is resistant to injury yet liable to strain, 24 hr following a single bout of eccentric exercise. Sca-1⁺CD45⁻ stem cells were increased 2-fold in WT muscle post-exercise. The α7 integrin regulated the presence of Sca-1⁺ cells, with expansion occurring in α7Tg muscle and minimal cells present in muscle lacking the α7 integrin. Sca-1⁺CD45⁻ cells isolated from α7Tg muscle following exercise were characterized as mesenchymal-like stem cells (mMSCs), predominantly pericytes. In vitro multiaxial strain upregulated mMSC stem cells markers in the presence of laminin, but not gelatin, identifying a potential mechanistic basis for the accumulation of these cells in muscle following exercise. Transplantation of DiI-labeled mMSCs into WT muscle increased Pax7⁺ cells and facilitated formation of eMHC⁺DiI⁻ fibers. This study provides the first demonstration that mMSCs rapidly appear in skeletal muscle in an α7 integrin dependent manner post-exercise, revealing an early event that may be necessary for effective repair and/or growth following exercise. The results from this study also support a role for the α7 integrin and/or mMSCs in molecular- and cellular-based therapeutic strategies that can effectively combat disuse muscle atrophy.
THE ROLE OF THE INSULIN-LIKE GROWTH FACTOR BINDING PROTEINS AND THE IGFBP PROTEASES IN MODULATING IGF ACTION (1996)

Over the past few years, there has been an explosion of data in the scientific literature regarding the various components of the IGF axis. IGFBPs and related molecules are now believed to be critical elements in numerous cellular processes and key factors in several disease states related to abnormal tissue and somatic growth. Recently, the BP-Prs were included in this complex system, and their importance is being unraveled. The upcoming years will undoubtedly bring even more information on the molecular biology of these key cellular regulators. These discoveries are likely to lead to better understanding of growth and cellular regulation and to development of novel therapeutic approaches to a variety of diseases.
Uniform uncertainty principle for Bernoulli and subgaussian ensembles (2006)

We present a simple solution to a question posed by Candes, Romberg and Tao on the uniform uncertainty principle for Bernoulli random matrices. More precisely, we show that a rectangular k*n random subgaussian matrix (with k &lt; n) has the property that by arbitrarily extracting any m (with m &lt; k) columns, the resulting submatrices are arbitrarily close to (multiples of) isometries of a Euclidean space. We obtain the optimal estimate for m as a function of k,n and the degree of "closeness" to an isometry. We also give a short and self-contained solution of the reconstruction problem for sparse vectors.
Noninvasive Assessment of the Right Ventricular Filling Pressure Gradient (2007)

For the first time, the RV driving filling force can be accurately measured noninvasively in the clinical setting, and the method is sensitive to detect the effects of preload, chronotropic, and lusitropic states. In patients with dilated cardiomyopathy, the RV filling force is markedly reduced, indicating severely impaired RV relaxation. These findings suggest that this is a useful tool for improving the clinical assessment of RV diastolic function.