gebhart/patrepeval huggingface dataset:
Genetically modified pigs as donors of cells, tissues, and organs for xenotransplantation (2019)

The number of donated human organs and tissues for patients with terminal organ failure falls far short of the need. According to the United Network for Organ Sharing (www.unos.org), more than 113,000 candidates for transplant are currently on the U.S. national waiting list, but only 36,527 organ transplants could be performed in 2018. Alternative sources, such as organs and tissues from animals, are therefore urgently needed. For a number or reasons, including size, anatomical, and physiological similarities with humans, the pig is the preferred donor species (reviewed in Cooper et al., 2016). Importantly, pigs can be optimized by genetic engineering as a source of cells, tissues, and organs for xenotransplantation. Recent advances in gen(om)e editing are speeding up progress in this field. Numerous genetically (multi-)modified pig lines have been generated to prevent immune rejection of xenotransplants, to overcome physiological incompatibilities, and to reduce the risk of transmitting zoonotic pathogens (Table 1; reviewed in Kemter et al., 2018).

Table 1.

Selection of genetic modifications of donor pigs for xenotransplantation
Specific unresponsiveness in rats with prolonged cardiac allograft survival after treatment with cyclosporine. Mediation of specific suppression by T helper/inducer cells. (1985)

DA rats grafted with major histocompatibility complex-incompatible PVG heart grafts and treated with cyclosporine (CY) for 10 d do not reject their grafts, and develop a state of specific unresponsiveness toward PVG allografts. Cells from these animals tested in an adoptive transfer assay were incapable of restoring PVG graft rejection, and capable of specifically inhibiting the capacity of adoptively transferred normal lymph node cells (LNC) to do so. They effected third party Wistar/Furth (W/F) graft rejection, however. Adoptive transfer assays with purified subpopulations of the lymphocytes that mediated this effect showed that W3/25+ T cells of the helper/inducer subclass, when injected alone, failed to restore rejection, and were also able, when injected with normal LNC or the W/25+ cells separated from them, to prevent these cells from effecting rejection. MRC OX8+ T cells of the cytotoxic/suppressor subclass, B cells, and serum from rats with long-surviving grafts all failed to inhibit the allograft responsiveness of normal LNC, and thus were not identified as mediators of the state of specific unresponsiveness. These results show that the specific unresponsiveness that develops in rats with long-surviving grafts, and which, in part at least, is responsible for prolonged graft survival, is due to an alteration in the alloreactivity of the helper/inducer subclass of T cells. These cells not only lack the capacity to initiate a rejection response against the alloantigens of the graft, but also have the ability to inhibit the capacity of normal W3/25+ cells to do so.
Molecular level photovoltaics: the electrooptical properties of metal cyanide complexes anchored to titanium dioxide (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular level photovoltaics: the electrooptical properties of metal cyanide complexes anchored to titanium dioxideTodd A. Heimer, Carlo A. Bignozzi, and Gerald J. MeyerCite this: J. Phys. Chem. 1993, 97, 46, 11987–11994Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/j100148a024https://doi.org/10.1021/j100148a024research-articleACS PublicationsRequest reuse permissionsArticle Views589Altmetric-Citations146LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
A Cubic, 12-Connected, Microporous Metal−Organometallic Phosphate Framework Sustained by Truncated Tetrahedral Nodes (2011)

A rare example of a microporous metal-organic phosphate, [Co(12)(L)(6)(μ(3)-PO(4))(4)(μ(3)-F)(4)(μ-H(2)O)(6)][NO(3)](2) (1), is synthesized by the reaction of a [(η(5)-C(5)H(5))Fe(II)](+)-functionalized terephthalate ligand with Co(NO(3))(2)·6H(2)O and phosphate and fluoride ions generated from the in situ hydrolysis of hexafluorophosphate. 1 is a cubic, 12-connected, face-centered cubic framework sustained by the linear connection of unprecedented, dodecanuclear truncated tetrahedral coordination clusters.
Granulopoiesis and colony stimulating factor production in regenerating liver. (1982)

Partial hepatectomy (hepx) of the adult animal induces a substantial hepatic erythropoietin (Ep) response following hypoxia and results in a transient reinitiation of hepatic erythropoiesis. This phenomenon is associated with an elevation in the number and activity of the hepatic macrophages or Kupffer cells. Kupffer cells have been implicated in the production not only of Ep, but of the moiety which regulates granulocyte growth in vitro, Colony Stimulating Factor (CSF). Histological examination of the regenerating liver revealed some foci of granulopoiesis, but not to the extent noted for erythropoiesis. Granulopoiesis was not associated with islet conformations involving central reticular or macrophagic cells. In addition, serum derived from the hepatic venous drainage of hepx animals possessed moderate levels of CSF, as determined by its ability to promote the growth of granulocytic-macrophagic colonies in agar culture.
Dielectrically-bonded long wavelength vertical cavity laser on GaAs substrates using strain-compensated multiple quantum wells (1994)

We present a novel low temperature bonding technique for fabricating long wavelength vertical cavity surface emitting lasers (VCSEL's). The technique relies on a 750 /spl Aring/-thick intermediate spin-on glass layer to join a highly efficient InP-based InGaAs-InGaAsP strain-compensated multiple quantum well (SC-MQW) gain medium on a GaAs substrate. We fabricated the device on GaAs in order to take advantage of highly reflective AlAs-GaAs Bragg reflectors. The optically-pumped device has a low threshold pump power of 4.2 kW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> at room temperature and operates at a wavelength of 1.44 μm.
Prefibrillar Amyloid Protein Aggregates Share Common Features of Cytotoxicity (2004)

The intracellular free Ca(2+) concentration and redox status of murine fibroblasts exposed to prefibrillar aggregates of the HypF N-terminal domain have been investigated in vitro and in vivo using a range of fluorescent probes. Aggregate entrance into the cytoplasm is followed by an early rise of reactive oxygen species and free Ca(2+) levels and eventually by cell death. Such changes correlate directly with the viability of the cells and are not observed when cell are cultured in the presence of reducing agents or in Ca(2+)-free media. In addition, moderate cell stress following exposure to the aggregates was found to be fully reversible. The results show that the cytotoxicity of prefibrillar aggregates of HypF-N, a protein not associated with clinical disease, has the same fundamental origin as that produced by similar types of aggregates of proteins linked with specific amyloidoses. These findings suggest that misfolded proteinaceous aggregates stimulate generic cellular responses as a result of the exposure of regions of the structure (such as hydrophobic residues and the polypeptide main chain) that are buried in the normally folded proteins. They also support the idea that a higher number of degenerative pathologies than previously known might be considered as protein deposition diseases.
The 3‐hydroxy‐3‐methylglutaryl co‐enzyme A reductase inhibitor pravastatin enhances neurite outgrowth in hippocampal neurons (2006)

Epidemiological studies demonstrate a relationship between statin [3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor] usage and reduced risk of developing Alzheimer's disease. To determine whether statins affect neuronal development, we treated cultured rat hippocampal neurons with pravastatin. After 4-48 h of treatment, pravastatin significantly increased the number of neurites produced by each cell and caused a corresponding increase in levels of the membrane phospholipid phosphatidylcholine. Pravastatin treatment also significantly increased neurite length and branching but did not affect cellular cholesterol levels. Co-incubation with mevalonate, but not cholesterol, abolished the stimulatory effect of pravastatin on neurite outgrowth. Treatment of neurons with isoprenoids also abolished the effect of pravastatin on neurite growth, suggesting that pravastatin may stimulate neuritogenesis by preventing isoprenylation of signaling molecules such as the Rho family of small GTPases. A specific inhibitor of geranylgeranylation, but not farnesylation, mimicked the stimulatory effect of pravastatin on neuritogenesis. Pravastatin treatment significantly decreased levels of membrane-associated RhoA. These data suggest that pravastatin treatment increases neurite outgrowth and may do so via inhibiting the activity of geranylgeranylated proteins such as RhoA.
Analysis of Pesticide Degradation Products by Tandem High-Performance Immunoaffinity Chromatography and Reversed-Phase Liquid Chromatography (1996)

The analysis of pesticide degradation in environmental samples is often difficult to perform due to the different polarities and lower concentrations of the degradation products versus the parent compound. This work examines the use of tandem high-performance immunoaffinity chromatography (HPIAC) and reversed-phase liquid chromatography (RPLC) for measuring degradation products of the herbicide atrazine in water at levels in the low nanogram per liter, or parts-per-trillion, range. An HPIAC column containing anti-triazine antibodies was first used to extract the degradation products of interest from samples, followed by the on-line separation of the retained components on a C18 RPLC analytical column. The final system had a total analysis time of 35 min for a 13 mL sample injection and gave good correlation versus GC/MS. The limits of detection for hydroxyatrazine, deethylatrazine, and deisopropylatrazine were 20−30 ng/L for 13 mL samples and 6−10 ng/L for 45 mL samples. The use of this method was demonstrated in the analysis of both river water and groundwater samples. By changing the HPIAC column and reversed-phase separation conditions, the same approach could be modified for the detection of other pesticides and their degradation products.
Single antenna interference cancellation for synchronised GSM networks using a widely linear receiver (2007)

A novel cochannel single antenna interference cancellation (SAIC) receiver is proposed for synchronised Group Special Mobile (GSM) systems. The receiver uses a two-stage strategy, where in the first stage cochannel interference is cancelled by a widely linear filter, while inter-symbol interference due to the GSM frequency-selective Rayleigh-fading environment is removed by a second-stage equaliser. Analytical results for the optimal widely linear filter coefficients are derived. Simulation results show excellent performance with large gains over the conventional receiver under interference limited channel conditions. It is shown that the conventional maximum likelihood sequence estimator or maximum a posteriori probability receiver is optimal when cochannel interference it not the dominant impairment, and it is proposed that the SAIC algorithm is disabled when the estimated carrier-to-interference (C/I) ratio is above a certain threshold.
Characterizing semen parameters and their association with reactive oxygen species in infertile men (2014)

Infertile men, irrespective of their clinical diagnoses, have reduced semen parameters and elevated ROS levels compared to proven fertile men who have established a pregnancy recently or in the past. Reactive oxygen species are negatively correlated with traditional semen parameters such as concentration, motility and morphology. Measuring ROS levels in the seminal ejaculates provides clinically-relevant information to clinicians.
Portable Electronic Nose Based on Electrochemical Sensors for Food Quality Assessment (2017)

The steady increase in global consumption puts a strain on agriculture and might lead to a decrease in food quality. Currently used techniques of food analysis are often labour-intensive and time-consuming and require extensive sample preparation. For that reason, there is a demand for novel methods that could be used for rapid food quality assessment. A technique based on the use of an array of chemical sensors for holistic analysis of the sample's headspace is called electronic olfaction. In this article, a prototype of a portable, modular electronic nose intended for food analysis is described. Using the SVM method, it was possible to classify samples of poultry meat based on shelf-life with 100% accuracy, and also samples of rapeseed oil based on the degree of thermal degradation with 100% accuracy. The prototype was also used to detect adulterations of extra virgin olive oil with rapeseed oil with 82% overall accuracy. Due to the modular design, the prototype offers the advantages of solutions targeted for analysis of specific food products, at the same time retaining the flexibility of application. Furthermore, its portability allows the device to be used at different stages of the production and distribution process.
Regulation of the Apolipoprotein AI Gene by ARP-1, a Novel Member of the Steroid Receptor Superfamily (1991)

Apolipoprotein AI (apoAI) is a lipid-binding protein that participates in the transport of cholesterol and other lipids in the plasma. A complementary DNA clone for a protein that bound to regulatory elements of the apoAI gene was isolated. This protein, designated apoAI regulatory protein-1 (ARP-1), is a novel member of the steroid hormone receptor superfamily. ARP-1 bound to DNA as a dimer, and its dimerization domain was localized to the COOH-terminal region. ARP-1 also bound to a thyroid hormone-responsive element and to regulatory regions of the apoB, apoCIII, insulin, and ovalbumin genes. In cotransfection experiments, ARP-1 downregulated the apoAI gene. The involvement of ARP-1 in the regulation of apoAI gene expression suggests that it may participate in lipid metabolism and cholesterol homeostasis.
Synthesis, Structure, and Single-Crystal EPR Study of [Cp(t-Bu3PN)Ti(μ-Cl)]2 (2000)

The reduction of CpTi(NPt-Bu3)Cl2 with Mg powder in benzene proceeds slowly to give [CpTi(NPt-Bu3(mu-Cl)]2 (1). An X-ray crystallographic study confirmed 1 is a centrosymmetric chloro-bridged dimer in the solid state. A single-crystal EPR study of 1 permitted the determination of the best-fitted values for the diagonal spin Hamiltonian parameters. This appears to be the first EPR single-crystal study of a Ti(III)-Ti(III) dimer. The implications of these data are considered and discussed in light of extended Hückel molecular orbital calculations.
The mobility and diffusion of ions in gases (1973)

Experimental and theoretical aspects of the mobility and diffusion of ions in gases are studied in detail. Some of the subjects discussed include ion-ion interaction, boundary condition and ion and electron behavior. Also discussed in separate chapters are the problems of the diffusion coefficients and the afterglow techniques. Finally, a special chapter studies the kinetic theory of diffusion and mobility, stressing the low-, medium- and high-field theory.
Construction of GFP vectors for use in Gram-negative bacteria other than Escherichia coli (1996)

A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519ngfp, broad host range mob+ plasmids containing gfp from a lac and an npt2 promoter, respectively.
VLSI architectures for video compression-a survey (1995)

The paper presents an overview on architectures for VLSI implementations of video compression schemes as specified by standardization committees of the ITU and ISO. VLSI implementation strategies are discussed and split into function specific and programmable architectures. As examples for the function oriented approach, alternative architectures for DCT and block matching will be evaluated. Also dedicated decoder chips are included Programmable video signal processors are classified and specified as homogeneous and heterogenous processor architectures. Architectures are presented for reported design examples from the literature. Heterogenous processors outperform homogeneous processors because of adaptation to the requirements of special, subtasks by dedicated modules. The majority of heterogenous processors incorporate dedicated modules for high performance subtasks of high regularity as DCT and block matching. By normalization to a fictive 1.0 /spl mu/m CMOS process typical linear relationships between silicon area and through-put rate have been determined for the different architectural styles. This relationship indicates a figure of merit for silicon efficiency.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
T cell epitopes of house dust mite major allergen Der p II. (1993)

Recent work has indicated the significance of IL-4- and IL-5-secreting allergen-specific human Th2 lymphocytes in the control of immune responses to allergens in atopic individuals. The precise allergenic epitopes that activate these allergen-specific Th2 cells are, however, hardly known. We analyzed the epitope-specificity of T lymphocytes specific for Der p II, one of the major allergens of house dust mite Dermatophagoides pteronyssinus. Using a panel of overlapping synthetic peptides that span the entire Der p II molecule, we could demonstrate that polyclonal Der p II-specific T cell lines prepared from the peripheral blood of five atopic patients can react with at least 10 different epitopes of the molecule. Each donor showed a different pattern of reactivity with the synthetic peptides, suggesting that Der p II contains multiple T cell epitopes that may differ from individual to individual. We studied the specificity of the T cell response to Der p II in more detail in one atopic patient using a short term polyclonal T cell line that strongly reacted to one single peptide (116-129) of the allergen. From this patient we established a panel of 11 Der p II-specific TLC. Ten TLC were of the CD3+ CD4+ phenotype and showed a high IL-4/IFN-gamma production ratio, whereas another TLC expressed CD3 and CD8 and failed to secrete substantial IL-4 and IFN-gamma. The use of at least four different TCR V beta gene segments was shown within this panel TLC. All TLC tested recognized the allergen in an HLA-DR1-restricted manner. Although this patient reacted to only one peptide on the polyclonal level, two T cell epitopes were identified on the clonal level by using synthetic peptides and autologous APC to stimulate the TLC. Combining data of CD4/CD8 expression, TCR V beta usage, and epitope specificity, at least six different types of Der p II-specific TLC could be identified within this patient. Binding of IgE to all synthetic peptides of Der p II is low and of low affinity, which may be of particular importance with respect to possible desensitization protocols using such peptides.
Pharmacokinetic analysis of plasma‐derived and recombinant F IX concentrates in previously treated patients with moderate or severe hemophilia B (2002)

The study found wide product- and patient-related variability in recovery. Inherent differences among patients, including baseline F IX, may account for some of the interpatient variability. These differences should be taken into account in optimizing treatment regimens for individual patients with hemophilia B.
Surface Sensitivity in Cluster-Ion-Induced Sputtering (2006)

The ion beam-induced removal of thin water ice films condensed onto Ag and bombarded by energetic Au, Au2, Au3, and C60 projectiles is examined both experimentally and with molecular dynamics computer simulations. For water overlayers of thicknesses greater than 10 A, the yields of sputtered Ag+ secondary ions decay exponentially with increasing ice thickness, revealing characteristic decay lengths of 24, 20, 18, and 7.0 A, respectively. It is shown that these values manifest the characteristic depths of projectile energy loss, rather than escape depths of the sputtered Ag atoms through the water ice overlayer. Computer simulations show that the mechanism of ejection involves the sweeping away of overlayer water molecules, allowing for an unimpeded escape of ejected Ag atoms. The relevance of these data with respect to surface sensitivity in secondary ion mass spectrometry is discussed.
(Invited) Experimental Evidence that the Plasma-Assisted MBE Growth of Nitride Alloys is a Liquid Phase Epitaxy Process (2011)

In this paper we propose that under Ga-rich conditions the growth of III-Nitride semiconductors by plasma-assisted MBE takes place though saturation of the metallic Ga covering the surface of the growing film with nitrogen, alloy constituents and impurities. According to this model the growth process is a liquid phase epitaxy (LPE) rather than vapor phase epitaxy. Experimental evidence is presented in support of this model. Such include, for example, that the growth rate of GaN does not decrease at temperatures in excess to 800 ºC, a result attributed to higher solubility of active nitrogen in Ga at higher temperatures. Also the metallic Ga in the surface of the film controls the incorporation of impurities and is responsible for the introduction of compositional inhomogeneities and thus band structure potential fluctuations in AlGaN due to lateral statistical fluctuations of the thickness of Ga in the surface.