gebhart/patrepeval huggingface dataset:
Suppressor of cytokine signaling 1 protects mice against concanavalin A–induced hepatitis by inhibiting apoptosis (2008)

These findings indicate that SOCS1 plays important negative roles in fulminant hepatitis and that forced expression of SOCS1 is therapeutic in preventing hepatitis.
FcγRIIB Ile232Thr transmembrane polymorphism associated with human systemic lupus erythematosus decreases affinity to lipid rafts and attenuates inhibitory effects on B cell receptor signaling (2005)

The B cell inhibitory receptor FcgammaRIIB plays crucial roles in the maintenance of self-tolerance. We have identified a polymorphism FCGR2B c.695T>C that results in the non-conservative replacement of 232Ile at the transmembrane helix to Thr and demonstrated the association of the polymorphism with susceptibility to systemic lupus erythematosus (SLE) in Asians. In this study, we examined the impact of FCGR2B c.695T>C on the functional properties of FcgammaRIIB by expressing each allele product in a human B cell line ST486 lacking endogenous FcgammaRIIB. FcgammaRIIB 232Thr was found to be significantly less potent than wild-type 232Ile in inhibiting B cell receptor (BCR)-mediated phosphatidylinositol-3,4,5-trisphosphate accumulation, Akt and PLCgamma2 activation and calcium mobilization, and to display decreased levels of tyrosine phosphorylation and SH2-containing 5'-inositolphosphate phosphatase recruitment compared with 232Ile after IgG Fc-mediated coligation with BCR. Notably, a quantitative analysis of the subcellular distribution of FcgammaRIIB using 125I-labeled anti-FcgammaRIIB revealed that FcgammaRIIB 232Thr is less effectively distributed to detergent-insoluble lipid rafts than 232Ile, findings in accordance with the importance of the transmembrane amino acid residues, in particular large hydrophobic amino acids including Ile, in the association of membrane proteins with lipid rafts. Given the crucial roles of lipid rafts in integrating BCR signaling, decreased association of FcgammaRIIB 232Thr could contribute to its impaired inhibitory potential. Collectively, the present findings indicate that the Ile232Thr substitution affects the localization and function of FcgammaRIIB and that the molecular mechanism may link the polymorphism and susceptibility to SLE.
High-titer HER-2/neu protein-specific antibody can be detected in patients with early-stage breast cancer. (1997)

The presence of HER-2/neu antibodies in breast cancer patients and the correlation with HER-2/neu-positive cancer implies that immunity to HER-2/neu develops as a result of exposure of patients to HER-2/neu protein expressed by their own cancer. These findings should stimulate further studies to develop the detection of immunity to oncogenic proteins as tumor markers, as well as the development and testing of vaccine strategies to induce and augment immunity to HER-2/neu for the treatment of breast cancer or prevention of recurrent disease.
Synthesis and sequence-specific DNA binding of a topoisomerase inhibitory analog of Hoechst 33258 designed for altered base and sequence recognition (1992)

The preparation and DNA binding characteristics of a structural analog of Hoechst 33258 bearing two pyridinic nitrogen atoms are described. The 1H NMR signals of the complex formed between the new ligand 1 and decadeoxyribonucleotide d(CATGGCCATG)2 were assigned by employing one- and two-dimensional NMR techniques. Intermolecular nuclear Overhauser effects (NOE) between the ligand and the DNA receptor fragment confirm that the ligand binds in the minor groove of the DNA, interacting with the centrally located 5'-GGCCA segment. In contrast to the steric clash between the benzimidazole rings of the parent Hoechst 33258 molecule and the guanine 2-NH2 groups, which renders it G.C avoiding and thus A.T base pair preferring, the ligand 1 described here overcomes these unfavorable interactions and instead exhibits a marked preference of G.C base pairs. This behavior appears to arise from additional stabilization due to H-bonding with the guanine 2-NH2 groups. Although a ligand-induced distortion at the binding site is qualitatively assessable, the overall B-type conformation of the DNA fragment is retained upon complexation. The structural conclusions drawn from the NOE-NMR evidence were confirmed by molecular mechanics and molecular modeling studies.
Cloning of a human complement component C4 gene. (1983)

Six overlapping cosmid clones having an average insert size of 40 kilobase pairs were identified and isolated from a human genomic library by using a cDNA probe, Alu-7, specific for the amino acid sequence of C4d, a known region of the fourth component of human complement. Analysis of these genomic clones by restriction digestion and Southern blotting shows that all six probably contain the same complete C4 gene. Nucleotide sequence comparison of the genomic clone Cos-A and the cDNA clone Alu-7 shows an identical sequence except for the presence of a 1,500-base-pair intron in the genomic sequence. The amino acid sequence predicted from the nucleotide sequence agrees with the known C4d region amino acid sequence with one exception.
Enhancing security and privacy in biometrics-based authentication systems (2001)

Because biometrics-based authentication offers several advantages over other authentication methods, there has been a significant surge in the use of biometrics for user authentication in recent years. It is important that such biometrics-based authentication systems be designed to withstand attacks when employed in security-critical applications, especially in unattended remote applications such as e-commerce. In this paper we outline the inherent strengths of biometrics-based authentication, identify the weak links in systems employing biometrics-based authentication, and present new solutions for eliminating some of these weak links. Although, for illustration purposes, fingerprint authentication is used throughout, our analysis extends to other biometrics-based methods.
An Improved Purification, Crystallization, and Some Properties of Rabbit Muscle 5'-Adenylic Acid Deaminase (1967)

A rapid method for the preparation of crystalline 5'-adenylic acid deaminase from rabbit skeletal muscle is presented.The enzyme remains bound to cellulose phosphate under conditions at which apparently no other proteins are bound; thus it was possible to develop, in essence, a one-step method for its purification.
A Facial Expression Parameterization by Elastic Surface Model (2009)

We introduce a novel parameterization of facial expressions by using elastic surface model. The elastic surface model has been used as a deformation tool especially for nonrigid organic objects. The parameter of expressions is either retrieved from existing articulated face models or obtained indirectly by manipulating facial muscles. The obtained parameter can be applied on target face models dissimilar to the source model to create novel expressions. Due to the limited number of control points, the animation data created using the parameterization require less storage size without affecting the range of deformation it provides. The proposed method can be utilized in many ways: (1) creating a novel facial expression from scratch, (2) parameterizing existing articulation data, (3) parameterizing indirectly by muscle construction, and (4) providing a new animation data format which requires less storage.
Measurement of Optical Path Length for Cerebral Near-Infrared Spectroscopy in Newborn Infants (1990)

The time taken for an extremely short pulse of near-infrared laser light to traverse the heads of 6 preterm infants was measured after death. The values obtained were used to calculate a differential path length factor (DPF), defined as the mean distance travelled by the photons divided by the distance between the points where light entered and left the head. The DPF was found to be 4.39 +/- 0.28. Knowledge of this factor will permit accurate quantitative measurements to be made by near-infrared spectroscopy of a range of indices of cerebral oxygenation and haemodynamics in live infants.
Obesity and Heart Failure — Risk Factor or Mechanism? (2002)

As has been noted repeatedly by expert panels and the lay press, nearly 60 percent of the adult population of the United States is overweight or obese, with substantial resulting morbidity and mortality.1 Since obesity increases the risk of the most frequent causes of heart failure, an association between these conditions is to be expected. Indeed, an increased risk of heart failure has previously been noted in the Framingham Heart Study and in other cohort studies.24 However, controversy has persisted with regard to whether obesity is an independent risk factor for heart failure or even coronary heart disease. For . . .
Generation of antibodies against human hsp27 and murine hsp25 by immunization with a chimeric small heat shock protein. (1991)

A hybrid protein containing the N-terminal part of the murine stress protein hsp25 (amino acids 1 to 110) and the C-terminal part of the human stress protein hsp27 (amino acids 111 to 208) was expressed in E. coli using a T7 polymerase/promoter system. The recombinant hybrid protein was purified and used for immunization of rabbits. In contrast to immunization experiments using hsp25 and hsp27 alone, immunization with the hybrid protein hsp25/27 leads to antibodies which can be used for detection of both hsp25 and hsp27.
The Changing Epidemiology of Vancomycin-Resistant Enterococcus (VRE) Bacteremia in Allogeneic Hematopoietic Stem Cell Transplant (HSCT) Recipients (2010)

The impact of the rising prevalence of vancomycin-resistant Enterococcus (VRE) prior to hematopoietic stem cell transplantation (HSCT) and changes in transplant techniques on risk of VREB (VRE bacteremia) early after HSCT is not known. This is a retrospective study of 247 adult patients who underwent allogeneic HSCT in the years 2008 and 2009 at the Memorial Sloan-Kettering Cancer Center. Sixty-eight of 247 (27.5%) patients were VRE colonized on pretransplant screening. VRE was the leading cause of bacteremia in the first 30 days after HSCT; 23 of 43 (53.5%) patients with positive blood cultures had VRE. Only 13 (57%) of the 23 patients with early VREB were colonized with VRE on pre-HSCT screening cultures. Mortality was directly attributable to VRE infection in 9% of patients with early VREB. VRE is emerging as the most common cause of preengraftment bacteremia in patients undergoing allogeneic HSCT, and is associated with substantial mortality. Pre-HSCT screening for VRE with stool cultures will not identify all patients who are at risk for VREB. The use of alternate agents with activity against Gram-positive bacteria for fever and neutropenia early after HSCT should be evaluated further in prospective studies.
Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody. (1989)

A mouse mAb, which recognized a rat T cell surface Ag responsible for the T cell activation, was produced by a regular hybridoma method using F344 rat T cells stimulated with PMA and a calcium ionophore, as the Ag. The mAb termed 1F4 (kappa-IgM) was reactive with rat T cells but not with B cells and immunohistochemically it stained rat thymus tissues strongly at medulla and weakly rat cortex. Addition of 1F4 mAb to a culture of T cells resulted in the proliferation of T cells by a help of PMA or a solid support. 1F4 mAb also caused the modulation of the corresponding Ag but not other T cell markers such as CD5, CD2, and OX-52-defined Ag. The 1F4 mAb immunoprecipitated a cell surface component having an apparent m.w. of 25,000 from rat T cells which could be associated with a disulfide-linked heterodimer (m.w. 92,000) consists of subunits having m.w. of about 52,000 and 43,000. These results strongly suggest that the 1F4 mAb recognizes a rat T cell Ag homologous to the human and mouse CD3.
Colour matching experiments with RGB‐LEDs (2008)

Abstract CIE colorimetry breaks down when lights produced by narrow band RGB‐LEDs are matched with broad‐band lights. A colour matching experiment was set up and matches in a number of parts of the chromaticity diagram have been made, to determine the magnitude of the discrepancy. Differences between visual and instrumental matches increase as one moves in the chromaticity diagram from yellowish white lights toward greenish and bluish lights. CIE TC 1‐36 recently suggested newly defined cone fundamentals: Applying a transformation of these to a space similar to the CIE XYZ space enables a much better prediction of the matches to be made. The difference between the visual match and its instrumental prediction decreases by a factor of two or even more. The use of a cone fundamental based colorimetric system is recommended for LED colorimetry. © 2008 Wiley Periodicals, Inc. Col Res Appl, 33, 108–112, 2008
Validation of Sterilization of Lucilia cuprina Larvae using Raw Honey through Scanning Electron Microscope (2020)

Background: In the application of maggot debridement therapy (MDT), sterilized maggots (Lucilia cuprina) by standard chemicals were used. Recent potential demands who want to use a procedure with all natural products, raw honey has been proposed as a natural sterilization technique to replace the chemical products.

Methods: Lucilia cuprina collected from a laboratory colony were divided into treated group (sterilized) and control group (non-sterilized) to undergo sterility testing with honey dilution. Scanning electron microscope (SEM) was used to examine and ascertain the sterility of honey dilution. SEM provided direct observation of microorganisms presence on external surface L. cuprina after sterilization by disinfectants of raw honey (tualang honey).

Results: Sixty maggots were examined with 30 in each group under SEM in three magnifications: ×300, ×1500, and ×6500. Treated group showed the absence of microorganism on the surface body segment of the larvae. It reflected in different magnifications in which treated maggots appeared to be smooth and clear of any external contaminants. The untreated maggots exhibited clusters of microorganisms on the external body surface.

Conclusion: This study confirmed the complete sterilization of maggots using raw honey as the antibacterial agent by using SEM due to its disinfection efficacy in destroying microorganisms in L.cuprina.
Allergic rhinitis: the “Ghost Diagnosis” in patients with asthma (2015)

Allergic rhinitis (AR) is a common comorbidity of asthma that contributes to asthma severity. Although over 80 % of asthmatics have AR, the condition is frequently underdiagnosed in subjects with asthma. AR itself is also a highly prevalent condition, affecting 10-30 % of adults and up to 40 % of children. AR has been associated with both increased risk of asthma development and asthma severity. The exact mechanisms underlying these relationships have yet to be fully elucidated, but evidence supports a role for allergen sensitization. Compared to those with asthma alone, patients with comorbid AR and asthma have greater use of health care resources, including visits to the general practitioner, emergency department and hospitalizations. Pharmacological treatment of AR reduces this health care burden. Immunotherapy for AR improves both asthma and rhinitis symptoms in addition to preventing future allergen sensitizations and asthma development. Appropriate recognition, diagnosis and treatment of AR can significantly reduce asthma morbidity and improve quality of life.
Sympathetic Augmentation in Hypertension (2003)

There is growing evidence that essential hypertension is commonly neurogenic and is initiated and sustained by sympathetic nervous system overactivity. Potential mechanisms include increased central sympathetic outflow, altered norepinephrine (NE) neuronal reuptake, diminished arterial baroreflex dampening of sympathetic nerve traffic, and sympathetic neuromodulation by angiotensin II. To address this issue, we used microneurography and radiotracer dilution methodology to measure regional sympathetic activity in 22 hypertensive patients and 11 normotensive control subjects. The NE transport inhibitor desipramine was infused to directly assess the potential role of impaired neuronal NE reuptake. To evaluate possible angiotensin sympathetic neuromodulation, the relation of arterial and coronary sinus plasma concentrations of angiotensin II to sympathetic activity was investigated. Hypertensive patients displayed increased muscle sympathetic nerve activity and elevated total systemic, cardiac, and renal NE spillover. Cardiac neuronal NE reuptake was decreased in hypertensive subjects. In response to desipramine, both the reduction of fractional transcardiac 3[H]NE extraction and the increase in cardiac NE spillover were less pronounced in hypertensive patients. DNA sequencing analysis of the NE transporter gene revealed no mutations that could account for reduced transporter activity. Arterial baroreflex control of sympathetic nerve traffic was not diminished in hypertensive subjects. Angiotensin II plasma concentrations were similar in both groups and were not related to indexes of sympathetic activation. Increased rates of sympathetic nerve firing and reduced neuronal NE reuptake both contribute to sympathetic activation in hypertension, whereas a role for dampened arterial baroreflex restraint on sympathetic nerve traffic and a peripheral neuromodulating influence of angiotensin II appear to be excluded.
Using cooperative MIMO techniques and UAV relay networks to support connectivity in sparse Wireless Sensor Networks (2013)

One possible way to define the end of lifetime for a Wireless Sensor Network (WSN) is to set a threshold for the number of disconnections among the sensor nodes so that above this level the WSN becomes unable to provide the quality of services required by the users or even totally loses its ability to provide any service at all. Disconnections isolate sensors or group of sensors which cannot deliver their acquired data, thus constituting a sparse nonfunctional WSN, although some of its isolated or grouped sensors remain operational. A possible way to overcome such a problem is to provide an alternative reliable connection via other types of nodes to support the communication among isolated parts of a disconnected network. This paper proposes the use of cooperative multiple input multiple output (MIMO) techniques to support communication among static sensors in a sparse WSN and a relay network composed of Unmanned Aerial Vehicles (UAVs) keeping the WSN connected, thus extending its lifetime. Simulations of the proposed approach are performed and the acquired results highlight the benefits of this proposal.
Transport Activity of MCT1 Expressed in Xenopus Oocytes Is Increased by Interaction with Carbonic Anhydrase (2005)

Injection of carbonic anhydrase isoform II (CA) into Xenopus frog oocytes increased the rate of H+ flux via the rat monocarboxylate transporter isoform 1 (MCT1) expressed in the oocytes. MCT1 activity was assessed by changes of intracellular H+ concentration measured by pH-selective microelectrodes during application of lactate. CA-induced augmentation of the rate of H+ flux mediated by MCT1 was not inhibited by ethoxyzolamide (10 microM) and did not depend on the presence of added CO2/HCO3- but was suppressed by injection of an antibody against CA. Deleting the C terminus of the MCT1 greatly reduced its transport rate and removed transport facilitation by CA. Injected CA accelerated the CO2/HCO3(-)-induced acidification severalfold, which was blocked by ethoxyzolamide and was independent of MCT1 expression. Mass spectrometry confirmed activity of CA as injected into the frog oocytes. With pulldown assays we demonstrated a specific binding of CA to MCT1 that was not attributed to the C terminus of MCT1. Our results suggest that CA enhances MCT1 transport activity, independent of its enzymatic reaction center, presumably by binding to MCT1.
Investigation of the morphology of the features generated via femtosecond lasers in the interior of a bovine cornea sections (2013)

Nonlinear absorption of femtosecond laser pulses enables the induction of bubble cavities in the interior of eye cornea without affecting other parts of an eye, a phenomena utilized for flap formation in laser assisted corneal surgery. In the present study laser pulses were focused in the interior of the sections of bovine cornea. Tight focus of the laser pulses results in the plasma formation followed by its explosive expansion, which drives cavity formation. The morphology of the generated features as well as the nature of the physical mechanisms of the phenomenon as a function of process parameters is discussed. Numerical model is proposed to develop predictive capabilities for the feature size and shape and the results are compared against the experimental findings.
Chiral N-heterocyclic carbene ligands based on a biisoquinoline template (2006)

New chiral imidazolinium salts with tunable steric features, based on a biisoquinoline template, have been developed and structurally characterised using single crystal X-ray crystallography. The trans PdI(2)(NHC)(2) complex was prepared by reaction of the parent H(4) imidazolinium salt with Pd(OAc)(2) in the presence of NaI, and the solid state structure determined by X-ray crystallography. The rigid, chiral, biisoquinoline geometry of the H(4) imidazolinium salt was found to be maintained upon ligand complexation. The sterically unencumbered parent biisoquinoline ligand has been found to give high conversion with modest enantioselectivity in the copper-catalysed asymmetric addition of diethylzinc to cyclohexenone.
A Cre-lox recombination system for the targeted integration of circular yeast artificial chromosomes into embryonic stem cells (2000)

The ability to produce embryonic stem (ES) cell lines containing different yeast artificial chromosomes (YACs) integrated into the same location in the genome provides a system for comparing the bio-logical effects of YAC transgenes without the confounding influences of integration site and copy number. A targeting system was developed for the directed integration of circular YACs into mouse ES cells. The system combines Cre-lox recombination technology, specifically a positive-selection integration system, with circular YAC lipofection technology to achieve single copy targeted integration of a transgene. Three independent germline competent ES cell lines [lox-containing ES lines (designated LES)] were created that contain a '-neo-lox' cassette integrated at different sites within the ES genome. A plasmid containing YAC vector sequences and a complementary '-neo-lox' cassette was used to circularize two linear YACs containing genomic DNA from human chromosome 21. The circularized YACs were then targeted to the lox sites of the LES cell lines. Polymerase chain reaction and Southern analysis demonstrated that 21% (5 of 24) of lox-recombinants contain a full-length intact YAC. This system will make the study of YAC transgenic mice more reliable and reproducible, allowing the potential for direct comparison of different transgenes expressed from the same site within the genome.
A physical map of the genome of Haemophilus influenzae type b (1990)

Contour-clamped homogeneous electric field pulsed-field gel electrophoresis (PFGE) was used in combination with Southern hybridization to construct a genomic restriction map for the pathogen Haemophilus influenzae serotype b, strain Eagan. Sites for four restriction endonucleases, EagI, NaeI, RsrII and SmaI, were located on the 2100 kbp circular chromosome. Twelve potential virulence loci have been placed on the map together with certain loci essential for growth of the bacteria (e.g. ribosomal RNA operons). PFGE also provided a valuable tool for characterizing ten capsulated, type b isolates (other than Eagan) known to be genetically heterogeneous and two laboratory-derived variants (transformants) derived through complex recombinational events involving random uptake of high-molecular-mass donor genomic DNA.