gebhart/patrepeval huggingface dataset:
Lipolysis of very low density lipoproteins by heparan sulfate proteoglycan-bound lipoprotein lipase (1997)

An in vitro assay to study lipolysis of very low density lipoproteins (VLDL) by heparan sulfate proteoglycan (HSPG-bound lipoprotein lipase (LPL) was developed. Optimal conditions for VLDL lipolysis by HSPG-bound LPL were obtained by incubating plastic wells with 0.5 pg HSPG and 1.5 pg LPL, subsequently. Control experiments with heparinase indicate that at least 90% of the LPL activity is derived from LPL bound to heparan sulfate chains. For HSPG-LPL-mediated lipolysis, the apparent & and V, , values were 0.36 It 0.1 1 mM VLDL-triglycerides and 1.2 2 0.1 p 4 free fatty acids/ min.ng LPL, respectively. The mean intra-assay and inter-assay coefficients of variance were 5% and 8%, respectively.-de Man, E H.
Combined immunization with DNA and transduced tumor cells expressing mouse GM-CSF or IL-2. (2005)

A combination of different types of vaccines usually induces enhanced immune responses in comparison to immunization with single vaccines. The highest efficacy of a heterologous prime-boost strategy is mostly achieved after priming with a DNA vaccine and boosting with a recombinant virus or a protein vaccine. The aim of this study was to determine whether the combination of a DNA and cellular vaccine elicits stronger antitumor immune responses than vaccines used alone and to find out whether the efficacy of this combined immunization depends on the sequence in which the vaccines were applied. We utilized experimental vaccines that proved to be partially effective in protection against mouse tumor cells representing models of human papillomavirus-induced malignancies. The fusion gene Sig/E7GGG/LAMP-1, inoculated via a gene gun, was used for DNA immunization. As cellular vaccines, HPV16 E6/E7 and H-ras transformed B9 or 181 cells transduced with the gene coding for GM-CSF or IL-2, respectively, were applied. In both preventive and therapeutic immunizations, inoculation first with the DNA vaccine followed by application of a cellular vaccine induced the best protection from tumor growth. These results were confirmed by detection of immune reactions with in vitro tests. We failed to enhance immune reactions by utilization of an equivalent mix of B9 and 181 cells, but the addition of the second DNA-vaccine dose applied simultaneously with a cellular-vaccine boost moderately increased antitumor response. Our findings suggest the benefit of the heterologous prime-boost strategy based on combination of a DNA vaccine with a cellular vaccine and importance of sequence in which the vaccines are administered.
Hexamethylenediamine (2012)

Abstract The article contains sections titled: 1. Introduction 2. Physical Properties 3. Chemical Properties 4. Production 5. Quality Specifications and Analysis 6. Storage and Transportation 7. Economic Aspects 8. Toxicology and Occupational Health
Considerations in the development of circulating tumor cell technology for clinical use (2012)

This manuscript summarizes current thinking on the value and promise of evolving circulating tumor cell (CTC) technologies for cancer patient diagnosis, prognosis, and response to therapy, as well as accelerating oncologic drug development. Moving forward requires the application of the classic steps in biomarker development-analytical and clinical validation and clinical qualification for specific contexts of use. To that end, this review describes methods for interactive comparisons of proprietary new technologies, clinical trial designs, a clinical validation qualification strategy, and an approach for effectively carrying out this work through a public-private partnership that includes test developers, drug developers, clinical trialists, the US Food & Drug Administration (FDA) and the US National Cancer Institute (NCI).
Lymphocyte binding to vascular endothelium in inflamed skin revisited: a central role for vascular adhesion protein‐1 (VAP‐1) (1996)

The binding of leukocytes to vascular endothelium and their migration into tissues is mediated by adhesion molecules on the endothelial cells and leukocytes. Vascular adhesion protein-1 (VAP-1) is a 170-180/90-kDa endothelial molecule expressed most prominently in high endothelial venules in peripheral lymph node (PLN) type lymphatic tissues. VAP-1 mediates lymphocyte binding to PLN, tonsil and synovium. The expression of VAP-1 is induced in inflammatory diseases such as arthritis and gut inflammation. We examined the expression, structure and function of VAP-1 in normal and inflamed skin and compared it to those of other adhesion molecules implicated in skin homing. In psoriasis lichen ruber planus, pemphigoid and allergic lesions, VAP-1 was markedly upregulated. The expression of VAP-1 was also increased in biopsies of healthy skin of the patients. The VAP-1 molecule induced in skin is decorated with abundant sialic acids. VAP-1 inflamed skin is functional, since inhibition with anti-VAP-1 monoclonal antibodies caused a 60% reduction in lymphocytes adhesion to vascular endothelium. Antibodies against E-selectin, which has been regarded as the major vascular addressin directing cutaneous lymphocyte traffic, and, surprisingly, against peripheral lymph node addressin (PNAd), caused inhibitions of 30% and 60%, respectively, in the frozen section adhesion assay. These findings suggest important roles also for VAP-1 and PNAd in lymphocyte homing into inflamed skin.
Incorporation of a Genetic Factor into an Epidemiologic Model for Prediction of Individual Risk of Lung Cancer: The Liverpool Lung Project (2010)

The Liverpool Lung Project (LLP) has previously developed a risk model for prediction of 5-year absolute risk of lung cancer based on five epidemiologic risk factors. SEZ6L, a Met430IIe polymorphic variant found on 22q12.2 region, has been previously linked with an increased risk of lung cancer in a case-control population. In this article, we quantify the improvement in risk prediction with addition of SEZ6L to the LLP risk model. Data from 388 LLP subjects genotyped for SEZ6L single-nucleotide polymorphism (SNP) were combined with epidemiologic risk factors. Multivariable conditional logistic regression was used to predict 5-year absolute risk of lung cancer with and without this SNP. The improvement in the model associated with the SEZ6L SNP was assessed through pairwise comparison of the area under the receiver operating characteristic curve and the net reclassification improvements (NRI). The extended model showed better calibration compared with the baseline model. There was a statistically significant modest increase in the area under the receiver operating characteristic curve when SEZ6L was added into the baseline model. The NRI also revealed a statistically significant improvement of around 12% for the extended model; this improvement was better for subjects classified into the two intermediate-risk categories by the baseline model (NRI, 27%). Our results suggest that the addition of SEZ6L improved the performance of the LLP risk model, particularly for subjects whose initial absolute risks were unable to discriminate into "low-risk" or "high-risk" group. This work shows an approach to incorporate genetic biomarkers in risk models for predicting an individual's lung cancer risk.
Duplex: a reusable fault tolerance extension framework for network access devices (2004)

A growing variety of edge network access devices appear on the marketplace that perform various functions which are meant to complement generic routers’ capabilities, such as firewalling, intrusion detection, virus scanning, network address translation, traffic shaping and route optimization. Because these edge network access devices are deployed on the critical path between a user site and its Internet service provider, high availability is crucial to their design. This paper describes the design, construction and evaluation of a general implementation framework for supporting fault tolerance on edge network devices. This implementation framework, called Duplex, is designed to be independent of the functionality of the hosting edge network access device, such that only a minimal amount of programming is required to tailor this framework to a specific edge network access device implementation. Duplex can tolerate power failure, hardware failure, and software failure by supporting device mirroring and watchdog timer-based link bypassing. Empirical performance measurements of an instance of Duplex that is embedded in a commercial bandwidth management device show that the run-time overhead of its fault tolerance mechanisms is less than 1 msec 90% of the time, and the failure detection and recovery period is less than 1.3 sec when running at 100 Mbps.
Hydrogen Sulphide Induces μ Opioid Receptor-Dependent Analgesia in a Rodent Model of Visceral Pain (2010)

This study provides pharmacological and molecular evidence that antinociception exerted by H2S in a rodent model of visceral pain is modulated by the transactivation of MOR. This observation provides support for development of new pharmacological approaches to visceral pain.
Tam zamanında üretim ortamında tedarikçi ilişkilerine dair bir uygulama (2014)

Tam Zamannda retimin (TZ) baars, gl tedariki ilikilerinin gelitirilmesine baldr.Tedariki seimi kriterleri bu nedenle nemlidir.TZ gibi btnleik bir yaklam benimseyen uygulamalar, tedarik zinciri yelerinin iletme performansn artracaktr
Machine Learning a General-Purpose Interatomic Potential for Silicon (2018)

A machine-learning based approach to computing the energy and forces of silicon atoms circumvents the need for complex electronic structure calculations, offering an efficient method for predicting material properties that can be extended to other materials.
Computer busses design and application (2000)

The computer bus is the foundation of the modern computer. Without busses, a computer would just be a bundle of components. As more and more equipment becomes interface driven-either through controllers or directly to and from PCs-the question of which bus to use becomes increasingly important.Computer Busses has been designed to help answer that question. It is a comprehensive guide to busses, from the high-level software interface to the low-level, electrical characteristics and will help you choose the most appropriate bus for each particular application. The author uses a top-down approach and organizes his presentation into five main areas:PC interfaces, Local busses, Instrumentation busses, Network busses, Bus programming and protocols.Plenty of real-life case studies and application-specific programming examples make Computer Busses an essential reference for engineers, system architects, designers, and technicians, and required reading for students doing project work in electronic design, software engineering, and the range of related disciplines.
Phage display peptide libraries: deviations from randomness and correctives (2018)

Peptide-expressing phage display libraries are widely used for the interrogation of antibodies. Affinity selected peptides are then analyzed to discover epitope mimetics, or are subjected to computational algorithms for epitope prediction. A critical assumption for these applications is the random representation of amino acids in the initial naïve peptide library. In a previous study, we implemented next generation sequencing to evaluate a naïve library and discovered severe deviations from randomness in UAG codon over-representation as well as in high G phosphoramidite abundance causing amino acid distribution biases. In this study, we demonstrate that the UAG over-representation can be attributed to the burden imposed on the phage upon the assembly of the recombinant Protein 8 subunits. This was corrected by constructing the libraries using supE44-containing bacteria which suppress the UAG driven abortive termination. We also demonstrate that the overabundance of G stems from variant synthesis-efficiency and can be corrected using compensating oligonucleotide-mixtures calibrated by mass spectroscopy. Construction of libraries implementing these correctives results in markedly improved libraries that display random distribution of amino acids, thus ensuring that enriched peptides obtained in biopanning represent a genuine selection event, a fundamental assumption for phage display applications.
Synthesis of 2-Thio- and 2-Oxoimidazoles via Cascade Addition−Cycloisomerization Reactions of Propargylcyanamides (2009)

A methodology to generate 2-thio- and 2-oxoimidazoles through an addition-cyclization-isomerization reaction of propargylcyanamides with thiol and alcohol nucleophiles is described. In general, the reaction sequence allows for the rapid formation of highly substituted 2-thio- and 2-oxoimidazoles in good to excellent yields.
Structured annotations of web queries (2010)

Queries asked on web search engines often target structured data, such as commercial products, movie showtimes, or airline schedules. However, surfacing relevant results from such data is a highly challenging problem, due to the unstructured language of the web queries, and the imposing scalability and speed requirements of web search. In this paper, we discover latent structured semantics in web queries and produce Structured Annotations for them. We consider an annotation as a mapping of a query to a table of structured data and attributes of this table. Given a collection of structured tables, we present a fast and scalable tagging mechanism for obtaining all possible annotations of a query over these tables. However, we observe that for a given query only few are sensible for the user needs. We thus propose a principled probabilistic scoring mechanism, using a generative model, for assessing the likelihood of a structured annotation, and we define a dynamic threshold for filtering out misinterpreted query annotations. Our techniques are completely unsupervised, obviating the need for costly manual labeling effort. We evaluated our techniques using real world queries and data and present promising experimental results.
Laser cooling investigation of a single-mode Yb-doped ZBLAN optical fiber (2017)

A theoretical study on laser cooling of a single-mode Yb3+:ZBLAN fiber is presented. Strategies to maximize the cooling efficiency are highlighted. The cooling scales quadratically with the core and inversely with the cladding radius
Repair of abdominal aortic aneurysms by the endoluminal method: outcome in the first 100 patients (1996)

Endoluminal repair of abdominal aortic aneurysms is feasible and this method of repair is durable in mid-term follow-up.
RITUXANTM/CHOP CHEMO IMMUNOTHERAPY IN PATIENTS WITH LOW-GRADE OR FOLLICULAR (LG/F) NON-HODGKINʼS LYMPHOMA (NHL) (1997)

Czuczman, M.; Grillo-Ló;pez, A. J.; White, C. A.; Saleh, M.; Gordon, L.; Lobuglio, A. F.; Jonas, C.; Shen, D.; Burris, K.; Dallaire, B. K.; Royston, I. Author Information
Pituitary radiographic abnormalities and clinical correlates of hypogonadism in elderly males presenting with erectile dysfunction (2002)

The prevalence of erectile dysfunction rises rapidly with age and is a frequent complaint presented in clinical practice. Although the etiology of erectile dysfunction is multifactorial, 10-20% of evaluations demonstrate testosterone deficiency. Testosterone deficiency due to secondary hypogonadism increases with age. Despite a higher prevalence of secondary hypogonadism in the elderly, there are no studies addressing hypothalamic-pituitary structural abnormalities in elderly impotent men with testosterone deficiency. We retrospectively reviewed the records of all elderly men who presented for general outpatient evaluation of erectile dysfunction from 1996 to 1999. To obtain a cohort control population, the records of 300 patients without erectile dysfunction were also reviewed. Amongst the erectile dysfunction patients, 225 were found to be testosterone deficient (testosterone < 300 ng/dl). Of these patients, 29 were additionally diagnosed with secondary hypogonadism based on a luteinizing hormone (LH) < 13 mIU/ml. Magnetic resonance imaging (MRI) or computed tomography (CT) imaging was available and reviewed in all patients diagnosed with secondary hypogonadism. Ten per cent of these patients had hypothalamic-pituitary imaging abnormalities. The prevalence of pituitary tumors within our population was not significantly elevated compared to the previous general population studies. Small-vessel white matter disease, hyperlipidemia and history of compression fractures were significantly increased in both univariate and multivariate analysis in the erectile dysfunction group compared with the control cohort. This study does not suggest that the use of hypothalamic-pituitary imaging in the evaluation of impotence in elderly men, in the absence of clinical characteristics of other hormonal loss or sella compression symptoms, will increase diagnosis of structural hypothalamic-pituitary abnormalities over that of the general population. However, the yield may increase with very low testosterone levels. These data suggest that there is an increase in ischemic white matter disease in elderly men with hypogonadism that may reflect microvascular injury to the hypothalamic-pituitary. Furthermore, these data confirm that low testosterone is associated with hyperlipidemia in the elderly. Future studies are required to assess the role of hypogonadism and hyperlipidemia, and to determine if treatment of the hormone deficiency improves the lipid profile.
Matrix surface modification by plasma polymerization for enzyme immobilization (1991)

Polycarbonate (PC) membranes have been treated with dimethylamine (DMA), n-pentylamine (PA) or n-heptylamine (HA) in a glow-discharge apparatus. Amino group concentrations on plasma-polymerized PC membranes were then assayed by binding radiolabelled [1-14C]acetic anhydride to the membrane, followed by scintillation counting. For the different plasma-polymerized membranes different degrees of radiolabelled (125I) glucose oxidase or rennet binding were observed and this was found to be directly related to the surface amino-group concentrations for the appropriate membrane.
IκB Kinase-β: NF-κB Activation and Complex Formation with IκB Kinase-α and NIK (1997)

Activation of the transcription factor nuclear factor kappa B (NF-kappaB) by inflammatory cytokines requires the successive action of NF-kappaB-inducing kinase (NIK) and IkappaB kinase-alpha (IKK-alpha). A widely expressed protein kinase was identified that is 52 percent identical to IKK-alpha. IkappaB kinase-beta (IKK-beta) activated NF-kappaB when overexpressed and phosphorylated serine residues 32 and 36 of IkappaB-alpha and serines 19 and 23 of IkappaB-beta. The activity of IKK-beta was stimulated by tumor necrosis factor and interleukin-1 treatment. IKK-alpha and IKK-beta formed heterodimers that interacted with NIK. Overexpression of a catalytically inactive form of IKK-beta blocked cytokine-induced NF-kappaB activation. Thus, an active IkappaB kinase complex may require three distinct protein kinases.
Dispersed disease-causing neomorphic mutations on a single protein promote the same localized conformational opening (2011)

The question of how dispersed mutations in one protein engender the same gain-of-function phenotype is of great interest. Here we focus on mutations in glycyl-tRNA synthetase (GlyRS) that cause an axonal form of Charcot-Marie-Tooth (CMT) diseases, the most common hereditary peripheral neuropathies. Because the disease phenotype is dominant, and not correlated with defects in the role of GlyRS in protein synthesis, the mutant proteins are considered to be neomorphs that gain new functions from altered protein structure. Given that previous crystal structures showed little conformational difference between dimeric wild-type and CMT-causing mutant GlyRSs, the mutant proteins were investigated in solution by hydrogen-deuterium exchange (monitored by mass spectrometry) and small-angle X-ray scattering to uncover structural changes that could be suppressed by crystal packing interactions. Significantly, each of five spatially dispersed mutations induced the same conformational opening of a consensus area that is mostly buried in the wild-type protein. The identified neomorphic surface is thus a candidate for making CMT-associated pathological interactions, and a target for disease correction. Additional result showed that a helix-turn-helix WHEP domain that was appended to GlyRS in metazoans can regulate the neomorphic structural change, and that the gain of function of the CMT mutants might be due to the loss of function of the WHEP domain as a regulator. Overall, the results demonstrate how spatially dispersed and seemingly unrelated mutations can perpetrate the same localized effect on a protein.
Adaptive Light Control - A New Light Concept Controlled by Vehicle Dynamics and Navigation (1998)

&lt;div class="htmlview paragraph"&gt;A new light concept called Adaptive Light Control (ALC) is developed with the aim to improve night time traffic safety. ALC improves the headlamp illumination by means of continuous adaption of the headlamps according to the current driving situation and current environment. In order to ensure rapid prototyping and early testing the step from offline to online (real-time) simulation of light distributions in the driving simulator has been successfully done. This realtime simulation enabled the interactive development of new light distributions in different driving situations and driving environments. The solutions are directly ported to real vehicels to allow further testing with natural road conditions.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;In this paper, results of the development of movable headlamps are presented. These headlamps are controlled by means of path prediction based on vehicle dynamics and route vectors of the navigation system. A comparison of static and dynamic light distributions is illustrated and an outline of the basic concept of the Adaptive Light Control system is given.&lt;/div&gt;
Micropipe Reactions in Bulk SiC Growth (2011)

M. Yu. Gutkin,1,2,3 T. S. Argunova,4,6 V. G. Kohn,5 A. G. Sheinerman1 and J. H. Je6 1Institute of Problems of Mechanical Engineering, RAS, St. Petersburg 2Department of Physics of Materials Strength and Plasticity, St. Petersburg State Polytechnical University, St. Petersburg 3Department of Theory of Elasticity, St. Petersburg State University, St. Petersburg 4Ioffe Physical-Technical Institute, RAS, St. Petersburg 5National Research Center ‘Kurchatov Institute’, Moscow 6X-ray Imaging Center, Department of Materials Science and Engineering, Pohang University of Science and Technology, Pohang 1,2,3,4,5Russia 6Republic of Korea
Fudging the Data (2009)

Abstract This chapter provides a number of examples of misrepresentations of medical history. It specifically shows that the claims made against the role of animals in biomedical research are simply false. It also considers the animal-rightist claims of those who really should know better, the medically trained. Ray and Jean Greek highlight in their book that they are not interested in animal rights but only in demonstrating that using animals in research endangers human health. They present the same distortions of medical history as those presented by other individuals discussed in this chapter. In addition, Brandon Reines specializes in advancing the thesis that significant medical advances are primarily due to observations made on human patients.