gebhart/patrepeval huggingface dataset:
Immunological tolerance to a defined myelin basic protein antigen administered intrathymically. (1994)

To explore the mechanisms responsible for the development of tolerance to allografts after intrathymic (IT) injection of alloantigen, the well-defined model of experimental autoimmune encephalomyelitis (EAE), which mimics the human autoimmune disease multiple sclerosis, was used. This inflammatory neurologic syndrome is initiated by myelin basic protein (MBP)-reactive CD4+ T lymphocytes restricted to self-MHC class II molecules. Naive adult, EAE-susceptible Lewis (RT1(1) rats were treated IT, i.v., or i.p. with a single dose (100 micrograms) of guinea pig-myelin basic protein (GP-MBP 1-176) in PBS plus 1 ml rabbit anti-rat lymphocyte serum i.p. Twenty-one days later, all rats were challenged by intradermal hind footpad injections of 50 micrograms GP-MBP in PBS emulsified in CFA. Only IT, but not i.p. or i.v., administration of GP-MBP plus anti-lymphocyte serum conferred marked resistance to a subsequent systemic challenge of GP-MBP, as demonstrated by the prevention of weight loss and paralysis characteristic of EAE. The IT administration dramatically decreased the size and number of histologic perivascular infiltrates observed per visual field in spinal cord of the tolerant animals and decreased GP-MBP-specific T lymphocyte in vitro proliferation (p < 0.01), whereas proliferation to a nonspecific mitogen (Con A) was not altered. With the addition of rIL-2, the decreased Ag-specific proliferative responses of IT-treated animals increased to control levels. Adoptive transfer of 100 x 10(6) splenocytes from tolerant hosts i.v. to naive syngeneic Lewis rats challenge with 100 micrograms GP-MBP in CFA had no effect on clinical or histologic EAE. Exposure of MBP to maturing thymocytes results in functionally immunounresponsive lymphocytes and prevention of autoimmune EAE.
Melt spinning of isotactic polypropylene: Structure development and relationship to mechanical properties (1977)

An extensive experimental study of structure development during the melt spinning of polypropylene and is as-spun polypropylene filaments is reported. Five polymers representing different molecular weights and polymerization methods were studied. WAXS, SAXS, and birefringence measurements were used to characterize the structure of the filaments. Spinning through air gives rise to monoclinic crystalline structures and spinning into cold water, the paracrystalline smectic form. Both crystalline and amorphous orientation factors were found to correlate with spinline stress for the different polymers studied. Mechanical properties of as-spun fibers such as modulus, yield strength, tensile strength, and elongation to break also correlate with spinline stress.
New synthetic methods. Secoalkylative approach to grandisol (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew synthetic methods. Secoalkylative approach to grandisolBarry M. Trost and Donald E. KeeleyCite this: J. Org. Chem. 1975, 40, 13, 2013Publication Date (Print):June 1, 1975Publication History Published online1 May 2002Published inissue 1 June 1975https://doi.org/10.1021/jo00901a039RIGHTS & PERMISSIONSArticle Views522Altmetric-Citations96LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (145 KB) Get e-Alerts Get e-Alerts
DeepFlow: Large Displacement Optical Flow with Deep Matching (2013)

Optical flow computation is a key component in many computer vision systems designed for tasks such as action detection or activity recognition. However, despite several major advances over the last decade, handling large displacement in optical flow remains an open problem. Inspired by the large displacement optical flow of Brox and Malik, our approach, termed Deep Flow, blends a matching algorithm with a variational approach for optical flow. We propose a descriptor matching algorithm, tailored to the optical flow problem, that allows to boost performance on fast motions. The matching algorithm builds upon a multi-stage architecture with 6 layers, interleaving convolutions and max-pooling, a construction akin to deep convolutional nets. Using dense sampling, it allows to efficiently retrieve quasi-dense correspondences, and enjoys a built-in smoothing effect on descriptors matches, a valuable asset for integration into an energy minimization framework for optical flow estimation. Deep Flow efficiently handles large displacements occurring in realistic videos, and shows competitive performance on optical flow benchmarks. Furthermore, it sets a new state-of-the-art on the MPI-Sintel dataset.
A New Type of Thermoalkalophilic Hydrolase of Paucimonas lemoignei with High Specificity for Amorphous Polyesters of Short Chain-length Hydroxyalkanoic Acids (2001)

A novel type of hydrolase was purified from culture fluid of Paucimonas (formerly Pseudomonas) lemoignei. Biochemical characterization revealed an unusual substrate specificity of the purified enzyme for amorphous poly((R)-3-hydroxyalkanoates) (PHA) such as native granules of natural poly((R)-3-hydroxybutyrate) (PHB) or poly((R)-3-hydroxyvalerate) (PHV), artificial cholate-coated granules of natural PHB or PHV, atactic poly((R,S)-3-hydroxybutyrate), and oligomers of (R)-3-hydroxybutyrate (3HB) with six or more 3HB units. The enzyme has the unique property to recognize the physical state of the polymeric substrate by discrimination between amorphous PHA (good substrate) and denatured, partially crystalline PHA (no substrate). The pentamers of 3HB or 3HV were identified as the main products of enzymatic hydrolysis of native PHB or PHV, respectively. No activity was found with any denatured PHA, oligomers of (R)-3HB with five or less 3HB units, poly(6-hydroxyhexanoate), substrates of lipases such as tributyrin or triolein, substrates for amidases/nitrilases, DNA, RNA, casein, N-alpha-benzoyl-l-arginine-4-nitranilide, or starch. The purified enzyme (M(r) 36,209) was remarkably stable and active at high temperature (60 degrees C), high pH (up to 12.0), low ionic strength (distilled water), and in solvents (e.g. n-propyl alcohol). The depolymerase contained no essential SH groups or essential disulfide bridges and was insensitive to high concentrations of ionic (SDS) and nonionic (Triton and Tween) detergents. Characterization of the cloned structural gene (phaZ7) and the DNA-deduced amino acid sequence revealed no homologies to any PHB depolymerase or any other sequence of data banks except for a short sequence related to the active site serine of serine hydrolases. A classification of the enzyme into a new family (family 9) of carboxyesterases (Arpigny, J. L., and Jaeger, K.-E. (1999) Biochem. J. 343, 177-183) is suggested.
Loss of IFN-γ Production by Invariant NK T Cells in Advanced Cancer (2001)

Invariant NK T cells express certain NK cell receptors and an invariant TCRalpha chain specific for the MHC class I-like CD1d protein. These invariant NK T cells can regulate diverse immune responses in mice, including antitumor responses, through mechanisms including rapid production of IL-4 and IFN-gamma, but their physiological functions remain uncertain. Invariant NK T cells were markedly decreased in peripheral blood from advanced prostate cancer patients, and their ex vivo expansion with a CD1d-presented lipid Ag (alpha-galactosylceramide) was diminished compared with healthy donors. Invariant NK T cells from healthy donors produced high levels of both IFN-gamma and IL-4. In contrast, whereas invariant NK T cells from prostate cancer patients also produced IL-4, they had diminished IFN-gamma production and a striking decrease in their IFN-gamma:IL-4 ratio. The IFN-gamma deficit was specific to the invariant NK T cells, as bulk T cells from prostate cancer patients produced normal levels of IFN-gamma and IL-4. These findings support an immunoregulatory function for invariant NK T cells in humans mediated by differential production of Th1 vs Th2 cytokines. They further indicate that antitumor responses may be suppressed by the marked Th2 bias of invariant NK T cells in advanced cancer patients.
cDNA cloning, chromosomal mapping, and functional characterization of the human peroxisome proliferator activated receptor (1993)

The human peroxisome proliferator activated receptor (hPPAR) was cloned from a human liver cDNA library. The cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity with mouse PPAR (mPPAR), respectively. The hPPAR gene was mapped on human chromosome 22 slightly telomeric to a linkage group of six genes and genetic markers that are located in the general region 22q12-q13.1. Cotransfection assays of mouse Hepa 1 cells were used to roughly compare the ability of hPPAR- and mPPAR-expressed cDNAs to trans-activate the acyl CoA oxidase (ACO) PPAR response element located 5' upstream to the minimal thymidine kinase promoter driving the expression of the chloramphenicol acetyl transferase (CAT) reporter gene. Both receptors elicited a response with the prototypical peroxisome proliferators nafenopin, clofibrate, and WY-14,643. Moreover, using cotransfection assays in which the CAT reporter plasmid contained the CYP4 A6 gene response element rather than the ACO element, it was shown that hPPAR is capable of very efficiently trans-activating a second PPAR response element. These results indicate that the PPAR is present in humans in a form that is functional and can trans-activate response elements derived from two different genes, the rat ACO and the rabbit CYP4A6.
Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction (2010)

The Diels-Alder reaction is a cornerstone in organic synthesis, forming two carbon-carbon bonds and up to four new stereogenic centers in one step. No naturally occurring enzymes have been shown to catalyze bimolecular Diels-Alder reactions. We describe the de novo computational design and experimental characterization of enzymes catalyzing a bimolecular Diels-Alder reaction with high stereoselectivity and substrate specificity. X-ray crystallography confirms that the structure matches the design for the most active of the enzymes, and binding site substitutions reprogram the substrate specificity. Designed stereoselective catalysts for carbon-carbon bond-forming reactions should be broadly useful in synthetic chemistry.
Increased Cerebral Cannabinoid-1 Receptor Availability Is a Stable Feature of Functional Dyspepsia: A [18F]MK-9470 PET Study (2015)

Although these findings need replication in larger samples, they suggest that the abnormal brain activity in several of these regions, previously demonstrated in FD, may be due to a sustained endocannabinoid system dysfunction, identifying it as a potential novel target for treatment and warranting further studies to elucidate whether it is also a feature of other FGIDs or FSSs.
Characterization of two DNA probes specific for Serpulina hyodysenteriae (1993)

Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with the nonpathogenic species Serpulina innocens or with other species of enteric bacteria, including Escherichia coli. Both probes are able to detect S. hyodysenteriae in colony blot hybridizations, and when applied to fecal specimens, they can detect 10(4) S. hyodysenteriae cells in 0.1 g of seeded fecal matter. Both probes can detect S. hyodysenteriae in fecal specimens from swine with clinical signs of swine dysentery after experimental challenge and from swine from a herd with an acute outbreak of swine dysentery. These probes have application as a diagnostic tool in veterinary microbiology.
Successful reduction of high-sustained anti-idursulfase antibody titers by immune modulation therapy in a patient with severe mucopolysaccharidosis type II (2014)

We report on a 6 year old boy with severe MPS II undergoing immune modulation therapy due to high IgG antibody titers to IV idursulfase and no significant decline in urinary GAG levels since initiating enzyme replacement therapy. He has complete deficiency of iduronate-2-sulfatase activity due to a submicroscopic deletion of the X chromosome involving the entire I2S gene but not including in the fragile X locus. At 19 months of age, IV idursulfase therapy at the recommended dose of 0.5 mg/kg/week was initiated and then increased to 1.0 mg/kg/week after no observed clinical improvement and no decline in urine GAG level. After one year of ERT at the increased dose, he had no significant decline in urinary GAG excretion and increase of anti-idursulfase IgG antibody titers to 102,000 with complete neutralizing antibodies. In light of the evidence of lack of efficacy of idursulfase therapy, the patient was started on an immune modulation regimen consisting of ofatumumab, bortezomib, methotrexate and IVIG for a 12 week period. Only a slight decrease in IgG titers and urine GAG levels was observed, leading to increased intensity of bortezomib administration and addition of dexamethasone to the regimen, while continuing with the current schedule ofatumumab, IVIG and methotrexate. Over 18 month period of immune modulation therapy, we observed a significant reduction in anti-idursulfase IgG titers and a moderate reduction in urine GAG levels compared to baseline. Modest clinical improvements were observed. Our experience suggests that future MPS II patients with a complete gene deletion may be likely to develop persistent anti-idursulfase antibody titers and may benefit from immune modulation therapy prior to the development of high titer levels.
HemaExplorer: a Web server for easy and fast visualization of gene expression in normal and malignant hematopoiesis (2012)

To the editor:

The HemaExplorer Web server allows for an easy display of mRNA expression profiles for query genes in murine and human hematopoietic stem and progenitor cells that represent consecutive developmental stages along the myeloid differentiation pathway. In addition, it provides the
Silicon microbial bioreactor arrays (2002)

The authors present a multi-sensor, multi-well bioreactor platform for high-throughput screening of microbial cultures. The platform is designed to interface directly with industry standard 96-well plates. Each well contains an independently addressable microfabricated sensor and actuator suite that provides closed-loop control over temperature, generates oxygen electrochemically and provides cell density data. Polysilicon, silicon nitride and metal films are deposited and patterned onto silicon wafers using standard silicon processing techniques. Results are presented for an 8-well cell density vs. temperature experiment. Additionally, the authors investigate the use of electrolysis as a means to generate oxygen for bacterial cultures.
Watermelon cultivation with fall-planted small grain plants intercropped as cover crop. (1999)

Wheat (Triticum aestivum L. cv. Mulchmugi) and barley (Hordeum vulgare L. cv. Minorimugi) were introduced in watermelon production for weed control, not seed production. To examine the weed control-effect of wheat and barley between rows of a watermelon field, they were sown in October, 1993, with the seeding rate of 4kg/10a. Elongated culms were mowed early in June, 1994, and turned into straw mulch. Light intensity (photon flux density) on the surface of the ground was suppressed by straw mulch of wheat and barley. Suppression of light intensity in wheat straw mulch 1 month after mowing was greater than that of barley because of the large biomass and its toughness. Matured seeds of the mowed culm germinated late in June, and new leaves developed above ground. Suppression of light intensity by new leaves of barley plants was more than that in wheat, and photon flux density on the surface of the ground was reduced to 30% of solar radiation. Weed quantity between rows was effectively reduced, especially by wheat straw mulch. Ground surface cover with triticale (x Triticosecale Wittmack cv. Ryeducks) was sparse because of fewer numbers of culms. Triticale was not suitable for the plant material of straw mulch in watermelon cultivation since the new shoots developed from the stubble after mowing and lifted the watermelon vines up. Intercropping system decreased the working hours for laying of straws and weedingin the field, and consequently led to labor saving.
Toward Bioelectronics:  Specific DNA Recognition Based on an Oligonucleotide-Functionalized Polypyrrole (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTToward Bioelectronics: Specific DNA Recognition Based on an Oligonucleotide-Functionalized PolypyrroleH. Korri-Youssoufi, F. Garnier, P. Srivastava, P. Godillot, and A. YassarView Author Information Laboratoire des Matériaux Moléculaires, CNRS 2 rue Dunant, 94320 Thiais, France Cite this: J. Am. Chem. Soc. 1997, 119, 31, 7388–7389Publication Date (Web):August 6, 1997Publication History Received10 December 1996Published online6 August 1997Published inissue 1 August 1997https://pubs.acs.org/doi/10.1021/ja964261dhttps://doi.org/10.1021/ja964261drapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views1408Altmetric-Citations293LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biopolymers,Electrodes,Genetics,Organic polymers,Oxidation Get e-Alerts
Peak power fluctuations in optical pulse compression (1988)

The effects of fluctuations in input intensity and pulsewidth on the output pulse of an optical pulse compressor are investigated. The performance of the fiber grating pulse compressor is modeled in the absence of group velocity dispersion. This model is verified by comparison to experimental results obtained with a compressed, continuous-wave, mode-locked Nd:YAG laser. An optimized servosystem is designed for the pulse-compressed laser system which results in as much as 50-dB noise reduction at low frequencies.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Near-infrared photocatalysis based on YF3 : Yb3+,Tm3+/TiO2 core/shell nanoparticles (2010)

We report the novel near-infrared (NIR) photocatalysis of YF(3) : Yb(3+),Tm(3+)/TiO(2) core/shell nanoparticles. The core/shell nanoparticles show photocatalytic activity under the NIR irradiation. This study demonstrates that the NIR energy can be used as the driving source for photocatalysis besides the UV and visible energy.
Gene delivery to respiratory epithelial cells by magnetofection (2004)

Magnetofection provides a potential novel method, which may overcome fundamental limitations of nonviral gene transfer to the airways. Due to the accelerated enrichment at the cell surface it may be of major interest for in vivo applications, where long-term incubation times at the target tissue are hardly achievable.
The Economics of Business Culture: Game Theory, Transaction Costs, and Economic Performance. (1994)

Journal Article The Economics of Business Culture: Game Theory, Transaction Costs, and Economic Performance Get access The Economics of Business Culture: Game Theory, Transaction Costs, and Economic Performance. By Casson (Mark). (Oxford and New York: Clarendon Press, 1991. Pp. xi + 286. £35.00 hardback. ISBN 0 19 828375 X.) D. P. O'Brien D. P. O'Brien University of Durham Search for other works by this author on: Oxford Academic Google Scholar The Economic Journal, Volume 104, Issue 425, 1 July 1994, Pages 949–950, https://doi.org/10.2307/2234991 Published: 01 July 1994
Oral vaccination using recombinant bacteria. (1990)

With the development of avirulent but immunogenic Salmonella strains it has become possible to deliver heterologous antigens to the mucosal and systemic immune system by the oral route. The basis of attenuation of different Salmonella mutants and the immune response to foreign antigens expressed by recombinant strains is discussed, as are problems related to the stabilisation of foreign genes in Salmonella and to the expression of immunogenic antigens.
Low-dose total skin electron beam therapy as a debulking agent for cutaneous T-cell lymphoma: an open-label prospective phase II study (2011)

Background Total skin electron beam therapy (TSEBT) is a powerful treatment for cutaneous T-cell lymphoma (CTCL). Based on the occurrence of relapses with low radiation doses, doses of 30-36 Gy are commonly used but most patients still eventually relapse and repeat treatment courses are limited due to the cumulative toxicity. Complete response (CR) rates are about 60-90% for T2-4 stages with a 5-year relapse-free survival of 10-25% for stages IB-III. Objectives To evaluate prospectively the efficacy of low-dose TSEBT (10 Gy) in terms of complete cutaneous response rate, overall response rate and response duration in CTCL. Methods Ten patients with stage IB-IV mycosis fungoides (MF) were treated in an open-label manner with four fractions of TSEBT 1 Gy weekly to a total skin dose of 10 Gy. Treatment responses were assessed at 1 and 3 months after treatment and subsequently at least every 6 months for a total period of 2 years or to disease relapse or progression. Results Patients achieved an overall response rate of 90%. The rate of CR or very good partial response (VGPR;
Prehospital Plasma during Air Medical Transport in Trauma Patients at Risk for Hemorrhagic Shock (2018)

In injured patients at risk for hemorrhagic shock, the prehospital administration of thawed plasma was safe and resulted in lower 30-day mortality and a lower median prothrombin-time ratio than standard-care resuscitation. (Funded by the U.S. Army Medical Research and Materiel Command; PAMPer ClinicalTrials.gov number, NCT01818427 .).