gebhart/patrepeval huggingface dataset:
Male Sterility and Partial Male Sterility in Sunflowers1 (1966)

Two types of genetic male sterility in sunflowers are described. The first, discovered at Bloomington, Indiana, produces no pollen or pollen grains of uneven size which are clumped and do not stain with aceto‐carmine. The second, found at Morden, Manitoba, always produces pollen. The grains are smaller than normal, nonstaining and not clumped. Three crosses of the Bloomington stock indicated the character is controlled by a single recessive gene and two others indicated duplicate genes. More consistent results with the Morden stock showed a single recessive gene governs the male sterility in it, and that this gene is not linked to a recessive gene for branching or to another for dwarf plant. The F 1 of reciprocal intercrosses of the two stocks was normal indicating that genetic control of the male sterility in them differs. Partial male sterility, characterized by varying proportions of normal pollen, occurred in breeding material of two lines. It appears possible to select lines with only 10 to 20% normal pollen and that such lines can be maintained easily. These lines cross well when naturally pollinated and it is concluded that they offer a better prospect for producing commercial hybrid seed of sunflowers than the genetic male sterility.
Nucleoticle sequence of a full-length cDNA (PST-1) for aryl sulfotransferase from rat liver (1990)

Journal Article Nucleoticle sequence of a full-length cDNA (PST-1) for aryl sulfotransferase from rat liver Get access Shogo Ozawa, Shogo Ozawa Department of Pharmacology, School of Medicine, Keio University35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Kiyoshi Nagata, Kiyoshi Nagata Department of Pharmacology, School of Medicine, Keio University35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Dawei Gong, Dawei Gong Department of Pharmacology, School of Medicine, Keio University35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Yasushi Yamazoe, Yasushi Yamazoe Department of Pharmacology, School of Medicine, Keio University35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Ryuichi Kato Ryuichi Kato Department of Pharmacology, School of Medicine, Keio University35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 13, 11 July 1990, Page 4001, https://doi.org/10.1093/nar/18.13.4001 Published: 11 July 1990
Towards End-to-End Learning for Dialog State Tracking and Management using Deep Reinforcement Learning (2016)

This paper presents an end-to-end framework for task-oriented dialog systems using a variant of Deep Recurrent Q-Networks (DRQN). The model is able to interface with a relational database and jointly learn policies for both language understanding and dialog strategy. Moreover, we propose a hybrid algorithm that combines the strength of reinforcement learning and supervised learning to achieve faster learning speed. We evaluated the proposed model on a 20 Question Game conversational game simulator. Results show that the proposed method outperforms the modular-based baseline and learns a distributed representation of the latent dialog state.
Clinical features and bacterial culture on stools of patients with acute diarrhea (2012)

Objective: To analyze clinical features and multi-bacteria culture result of acute diarrhea patients.Methods: Among the acute diarrhea patients who seek medical advice in intestinal outpatient service in Dongfang Hospitital and Youan Hospitital from April in 2010 to March in 2011,158 cases were randomly selected carry out the dung bacteria culture,then their clinical diagnosis,clinical features and the multi-bacteria culture result were analyzed.Results: Among the 158 cases,60 cases were acute enteritis,17 cases were acute bacterial dysentery,58 cases were gastrointestinal dysfunction,7 cases were indigestion,and 12 cases were diarrhea referred;41 cases were found dung bacteria culture positive(25.95%,41/158),which consist of 17 cases of(41.46%,17/41)Vibrio parahaemolyticus,10 cases of Shigella(24.39%,10/41),7 cases of Salmonella(17.07%,7/41),4 cases of plesiomonas shigelloides(9.76%,4/41),2 cases of aeromonas(4.88%,2/41)and 1 cases of vibrio Cholerae(2.44%,1/41).The clinical diagnosis of the 41 cases whose dung bacteria culture were positive,including 24 case of acute enteritis,10 case of acute bacterial dysentery,3 case of gastrointestinal dysfunction,1 case of indigestion,and 1 case diarrhea referred.Conclusion: The main pathogenic bacteria of acute diarrhea in Fengtai area of Beijing was Vibrio parahaemolyticus, followed by Shigella;the clinical diagnosis of acute diarrhea often excessively rely on the conventional chemical examination result of dung,which was worth paying attention by clinicians.
Indium-111-DTPA-folate as a potential folate-receptor-targeted radiopharmaceutical. (1998)

Indium-111-DTPA-folate appears suitable as a radiopharmaceutical for targeting tumor-associated folate receptors.
The Rational Discovery of a Tau Aggregation Inhibitor (2018)

Intrinsically disordered proteins play vital roles in biology, and their dysfunction contributes to many major disease states. These proteins remain challenging targets for rational ligand discovery or drug design because they are highly dynamic and fluctuate through a diverse set of conformations, frustrating structure-based approaches. To meet this challenge, we have developed protocols to efficiently identify active small molecule ligands of disordered proteins. Our approach utilizes enhanced sampling molecular dynamics and conformational analysis approaches optimized for disordered targets, coupled with computational docking and machine learning-based screens of compound libraries. By applying this protocol to an amyloid-forming segment of microtubule-associated protein tau, we successfully identified novel, chemically diverse tau ligands, including an inhibitor that delays the aggregation reaction in vitro without affecting the amount of aggregate formed at the steady state. Our results indicate that we have expanded the toolkit of protein aggregation inhibitors into new areas of chemical space and demonstrate the feasibility of our ligand discovery strategy.
Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production. (1983)

FU-266, a mutant human myeloma cell line sensitive to hypoxanthine/aminopterin/thymidine (HAT), was transfected by protoplast fusion with DNA of the recombinant plasmid vector pSV2-neoR, thus acquiring a dominant marker conferring resistance to the antibiotic G-418. One of the resultant neoR clones, E-1, was fused to irradiated (500 rads) or unirradiated cells of the HAT-sensitive, G-418-sensitive, nonproducer mouse myeloma line X63-Ag8.653. Hybrid clones were selected in G-418 plus ouabain, thus preserving their HAT sensitivity. Small numbers of human chromosomes were retained in all such hybrids, but most of them ceased secreting human myeloma (IgE(lambda). Selected hybrid clones were then tested as malignant fusion partners in a series of fusions with polyclonally activated human B lymphocytes and with antigen-primed human B lymphocytes, in some instances after transformation of the latter with Epstein-Barr virus. The yield of viable chimeric hybridomas has been consistently high, as has the proportion of hybridomas secreting human immunoglobulin molecules unpermuted with mouse or human myeloma heavy or light chains. Secretion by many subcloned hybridomas has been stable for over 6 months, and several antigen-specific human monoclonal antibodies have been generated. Thus these heteromyeloma cell lines appear to have significant advantages for human monoclonal antibody production.
Remote-Conditioning Ischemia Provides a Potential Approach to Mitigate Contrast Medium-Induced Reduction in Kidney Function: A Retrospective Observational Cohort Study (2011)

Contrast medium administration during imaging and therapeutic procedures can cause renal injury, partly due to ischemia. Therefore, we hypothesized that brief ischemia and reperfusion episodes applied at a distant site - multiple balloon inflations and deflations during angioplasty - may serve as a remote-conditioning (RC) stimulus and thereby protect against contrast-induced kidney injury. To test this hypothesis, we (1) utilized cases from a prior study in which patients undergoing emergent angioplasty for ST segment elevation myocardial infarction received either 1-3 balloon inflations (controls) or were 'conditioned' with multiple (≥4) inflations, and (2) assessed renal function for 3 days in patients with an estimated glomerular filtration rate (eGFR) of <90 ml/min/1.73 m(2) prior to revascularization (mild kidney disease). Both groups displayed increased eGFR at day 1 after angioplasty versus baseline; attributed to in-hospital hydration (control: 77 ± 14 vs. 68 ± 12 ml/min/1.73 m(2); p < 0.01; RC: 81 ± 21 vs. 69 ± 12 ml/min/1.73 m(2); p < 0.01). In controls, this improvement was transient: eGFR subsequently decreased to 70 ± 14 ml/min/1.73 m(2) at day 3 (p < 0.05). In contrast, the RC group (despite receiving 25% more contrast volume) showed no functional decline at day 3 (80 ± 14 ml/min/1.73 m(2)). These results are consistent with remote ischemic conditioning providing a novel potential approach to attenuate contrast-associated renal injury.
Coding region of NKX3.1, a prostate-specific homeobox gene on 8p21, is not mutated in human prostate cancers. (1997)

Loss of heterozygosity at chromosome 8p21-22 is common in human prostate cancer, suggesting the presence of one or more tumor suppressor genes at this locus. A homeobox gene that is expressed specifically in adult human prostate, NKX3.1, the expression of which is regulated by androgen, maps to chromosome 8p21. Fine structure in situ mapping showed that NKX3.1 is proximal to MSR32 (macrophage scavenger receptor type II) and LPL (human lipoprotein lipase) and very close to NEFL (human neurofilament light chain) on 8p21. Single-strand conformational polymorphism analysis of 48 radical prostatectomy cancer specimens and 3 metastases for the entire coding region of NKX3.1 showed no tumor-specific sequence alterations in 50 specimens and total absence of the gene in 1 specimen known to have a biallelic deletion of 8p21. NKX3.1 was found to have a polymorphism at nucleotide 154 in codon 52 that resulted in a CGC-->TGC sequence change and an Arg-->Cys amino acid alteration (R52C). This polymorphism was present in 20% of DNA samples. If NKX3.1 is a target of the 8p21 LOH, it is not via disruption of the coding region of the gene.
Bioreactor Development for Production of Viral Pesticides or Heterologous Proteins in Insect Cell Culturesa (1990)

The insect cell-baculovirus expression system has significant potential for producing proteins requiring some degree of posttranslational modification. T. ni cells appear to be as good a host as S. frugiperda cells for heterologous protein production as demonstrated by production of beta-galactosidase. Attachment-dependent cells of T. ni can be effectively cultured in a packed-bed reactor using glass beads. When cell in such a reactor were infected, they produced 35% of the total protein as beta-galactosidase. No cell detachment was observed even 70 h postinfection. A model of viral entry has been proposed and tested.
RBTT-03. A PHASE 1, MULTICENTER, RANDOMIZED, OPEN-LABEL, PERIOPERATIVE STUDY OF AG-120 (IVOSIDENIB) AND AG-881 IN PATIENTS WITH RECURRENT, NONENHANCING, IDH1-MUTANT, LOW-GRADE GLIOMA (2018)

Mutations in isocitrate dehydrogenase (mIDH) are common in lower-grade glioma (LGG; mIDH1, 80%; mIDH2, 4%) and lead to epigenetic and genetic changes that promote oncogenesis via production of the oncometabolite 2-hydroxyglutarate (2-HG). AG-120 (ivosidenib) is a first-in-class oral mIDH1 inhibitor associated with a favorable safety profile in an ongoing phase 1 study in 66 glioma patients. AG-881 is a brain-penetrant oral mIDH1/2 inhibitor with an acceptable safety profile at dose levels <100 mg in an ongoing phase 1 study in 52 glioma patients. In an orthotopic mIDH1-R132H glioma model, ivosidenib and AG-881 suppressed 2-HG by 85% and 98%, respectively. This multicenter, open-label, phase 1 study is designed to measure brain tumor 2-HG concentration and drug exposure at two dose levels each of ivosidenib or AG-881 in patients with mIDH1 LGG undergoing craniotomy (NCT03343197). The study will enroll ~45 adults with recurrent WHO 2016-classified Grade 2 or 3 mIDH1 oligodendroglioma/astrocytoma eligible for resection. Key eligibility criteria include: nonenhancing disease by T2 FLAIR MRI, mIDH1-R132H, and Karnofsky Performance Score 60. Cohort 1 patients will be randomized 2:2:1 to 500 mg QD ivosidenib (n=10), 50 mg QD AG-881 (n=10), or no treatment (n=5). Based on the safety, 2-HG, and pharmacokinetic data from cohort 1, cohort 2 may enroll an additional 20 patients randomized 1:1 to 250 mg BID ivosidenib or 10 mg QD AG-881. Patients randomized to either drug will receive treatment for 4 weeks preoperatively and may continue treatment after surgery. Untreated patients can opt to be randomized to either drug postoperatively. The primary endpoint is 2-HG concentration in surgically resected tumors. Secondary endpoints include safety, tumor and plasma pharmacokinetics, and RANO LGG response. Exploratory endpoints include 2-HG and pharmacokinetics in cerebrospinal fluid, and 2-HG magnetic resonance spectroscopy. This study is currently enrolling in the USA.
Characterization of fluid flow velocity by optical Doppler tomography (1995)

The spatial profiles of fluid flow velocity in transparent glass and turbid collagen conduits are measured by optical Doppler tomography (ODT). The flow velocity at a discrete user-specified spatial location in the conduit is determined by measurement of the Doppler shift of backscattered light from microspheres suspended in the flowing fluid. Experimental data and theoretical calculations are in excellent agreement. ODT is an accurate method for the characterization of high-resolution fluid flow velocity.
New supramolecular packing motifs: π-stacked rods encased in triply-helical hydrogen bonded amide strands (1999)

The structure of N,N′,N″-tris(2-methoxyethyl)benzene-1,3,5-tricarboxamide consists of aryl rings self-assembled using a novel conjunction of organizational motifs into a π-stack surrounded by a triple helical network of hydrogen bonds, in a manner suggestive of a new mode of organization for some columnar liquid crystals.
Temporal Variation in Incidence of IDDM in Canterbury, New Zealand (1992)

IDDM in Canterbury, New Zealand, presents in cycles of incidence peaks and troughs, each spanning 2-3 yr.
The production of myco-diesel hydrocarbons and their derivatives by the endophytic fungus Gliocladium roseum (NRRL 50072) (2010)

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. This supports our principal goal to develop, expand and strengthen the networks available to our members so that they can generate new knowledge about microbes and ensure that it is shared with other communities.
Rescue of Dystrophic Muscle Through U7 snRNA-Mediated Exon Skipping (2004)

Most mutations in the dystrophin gene create a frameshift or a stop in the mRNA and are associated with severe Duchenne muscular dystrophy. Exon skipping that naturally occurs at low frequency sometimes eliminates the mutation and leads to the production of a rescued protein. We have achieved persistent exon skipping that removes the mutated exon on the dystrophin messenger mRNA of the mdx mouse, by a single administration of an AAV vector expressing antisense sequences linked to a modified U7 small nuclear RNA. We report the sustained production of functional dystrophin at physiological levels in entire groups of muscles and the correction of the muscular dystrophy.
Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens. (1986)

We used a murine retrovirus shuttle vector system to construct recombinants capable of constitutively expressing the simian virus 40 (SV40) large T antigen and the polyomavirus large and middle T antigens as well as resistance to G418. Subsequently, these recombinants were used to generate cell lines that produced defective helper-free retroviruses carrying each of the viral oncogenes. These recombinant retroviruses were used to analyze the role of the viral genes in transformation of rat F111 cells. Expression of the polyomavirus middle T antigen alone resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were highly tumorigenic, whereas expression of the polyomavirus large T resulted in cell lines that were unaltered by the criteria of morphology, anchorage-independent growth, and tumorigenicity. More surprisingly, SV40 large T-expressing cell lines were not tumorigenic despite the fact that they contained elevated levels of cellular p53 and had a high plating efficiency in soft agar. These results suggest that the SV40 large T antigen is not an acute transforming gene like the polyomavirus middle T antigen but is similar to the establishment genes such as myc and adenovirus EIa.
Radiative cooling of solar absorbers using a visibly transparent photonic crystal thermal blackbody (2015)

A solar absorber, under the sun, is heated up by sunlight. In many applications, including solar cells and outdoor structures, the absorption of sunlight is intrinsic for either operational or aesthetic considerations, but the resulting heating is undesirable. Because a solar absorber by necessity faces the sky, it also naturally has radiative access to the coldness of the universe. Therefore, in these applications it would be very attractive to directly use the sky as a heat sink while preserving solar absorption properties. Here we experimentally demonstrate a visibly transparent thermal blackbody, based on a silica photonic crystal. When placed on a silicon absorber under sunlight, such a blackbody preserves or even slightly enhances sunlight absorption, but reduces the temperature of the underlying silicon absorber by as much as 13 °C due to radiative cooling. Our work shows that the concept of radiative cooling can be used in combination with the utilization of sunlight, enabling new technological capabilities.
A Systematic Review of Digital and Computer-Based Alcohol Intervention Programs in Primary Care (2015)

This is the first systematic review of digital or computer-based alcohol intervention programs used by primary care patients. Although the number of trials in this area of research is relatively small, their findings indicate effectiveness in reducing alcohol consumption as well as drinking related consequences. Extended interventions which offer additional opportunities to monitor drinking over time appear no more effective than single occasion brief interventions. These findings suggest that digital and computer-based interventions in primary care may have the potential to increase alcohol intervention activities in the general population at a relatively low cost and low burden on practitioners.
In vitro-selected RNA cleaving DNA enzymes from a combinatorial library are potent inhibitors of HIV-1 gene expression (2000)

Selective inactivation of a target gene by antisense mechanisms is an important biological tool to delineate specific functions of the gene product. Approaches mediated by ribozymes and RNA-cleaving DNA enzymes (DNA enzymes) are more attractive because of their ability to catalytically cleave the target RNA. DNA enzymes have recently gained a lot of importance because they are short DNA molecules with simple structures that are expected to be stable to the nucleases present inside a mammalian cell. We have designed a strategy to identify accessible cleavage sites in HIV-1 gag RNA from a pool of random DNA enzymes, and for isolation of DNA enzymes. A pool of random sequences (all 29 nucleotides long) that contained the earlier-identified 10-23 catalytic motif were tested for their ability to cleave the target RNA. When the pool of random DNA enzymes was targeted to cleave between any A and U nucleotides, DNA enzyme 1836 was identified. Although several DNA enzymes were identified using a pool of DNA enzymes that was completely randomized with respect to its substrate-binding properties, DNA enzyme-1810 was selected for further characterization. Both DNA enzymes showed target-specific cleavage activities in the presence of Mg(2+) only. When introduced into a mammalian cell, they showed interference with HIV-1-specific gene expression. This strategy could be applied for the selection of desired target sites in any target RNA.
Active wavelength measurement system using an InGaAs-InP quantum-well electroabsorption filtering detector (1996)

A novel wavelength measurement system based on an InGaAs-InP multiple quantum-well electroabsorption filtering detector has been demonstrated. The tunable wavelength response of the filtering detector is used in an active manner to track the wavelength of a spectrally narrow input optical signal. An average resolution of /spl plusmn/8.30 pm was achieved, over a measurement range of 43 nm.
Expression and Function of Murine Receptor Tyrosine Kinases, TIE and TEK, in Hematopoietic Stem Cells (1997)

Two highly related receptor tyrosine kinases, TIE and TEK, comprise a family of endothelial cell-specific kinase. We established monoclonal antibodies against them and performed detailed analyses on their expression and function in murine hematopoietic stem cells (HSCs). TIE and TEK were expressed on 23.7% and 33.3% of lineage marker-negative, c-Kit+ and Sca-1+ (Lin- c-Kit+ Sca-1+) HSCs that contain the majority of day-12 colony-forming units-spleen (CFU-S) and long-term reconstituting cells, but not committed progenitor cells. Lin- c-Kit+ Sca-1+ cells were further divided by the expression of TIE and TEK. TIE+ and TEK+ HSCs as well as each negative counterpart contained high proliferative potential colony-forming cells and differentiated into lymphoid and myeloid progenies both in vitro and in vivo. However, day-12 CFU-S were enriched in TIE+ and TEK+ HSCs. Our findings define TIE and TEK as novel stem cell marker antigens that segregate day-12 CFU-S, and provide evidence of novel signaling pathways that are involved in the functional regulation of HSCs at a specific stage of differentiation, particularly of day-12 CFU-S.