gebhart/patrepeval huggingface dataset:
Increase in respiratory cost at high growth temperature is attributed to high protein turnover cost in Petunia × hybrida petals (2007)

It is widely believed that turnover of nitrogenous (N) compounds (especially proteins) incurs a high respiratory cost. Thus, if protein turnover costs change with temperature, this would influence the dependence of respiration rate on growth temperature. Here, we examined the extent to which protein turnover cost explained differences in N-utilization costs (nitrate uptake/reduction, ammonium assimilation, amino acid and protein syntheses, protein turnover and amino acid export) and in respiration rate with changes in growth temperature. By measurements and literature data, we evaluated each N-utilization cost in Petunia x hybrida petals grown at 20, 25 or 35 degrees C throughout their whole lifespans. Protein turnover cost accounted for 73% of the integrated N-utilization cost on a whole-petal basis at 35 degrees C. The difference in this cost on a dry weight basis between 25 and 35 degrees C accounted for 75% of the difference in N-utilization cost and 45% of the difference in respiratory cost. The cost of nitrate uptake/reduction was high at low growth temperatures. We concluded that respiratory cost in petals was strongly influenced by protein turnover and nitrate uptake/reduction, and on the shoot basis, C investment in biomass was highest at 25 degrees C.
Cell Cycle-dependent Expression and Spindle Pole Localization of a Novel Human Protein Kinase, Aik, Related to Aurora of Drosophila and Yeast Ipl1 (1997)

Mutations in Aurora of Drosophila and related Saccharomyces cerevisiae Ipl1 kinase are known to cause abnormal chromosome segregation. We have isolated a cDNA encoding a novel human protein kinase of 402 amino acids with a predicted molecular mass of 45.9 kDa, which shares high amino acid identities with the Aurora/Ipl1 protein kinase family; hence the cDNA is designated as aik (aurora/IPL1-related kinase). Amino acid sequence of C-terminal kinase domain of Aik shares 86, 86, 72, 59, and 49% identity with those of Xenopus XLP46APK and XLP46BPK, mouse STK-1, Aurora of Drosophila, and yeast Ipl1, respectively, whereas N-terminal domain of Aik shares high homology only with those of XLP46APK and XLP46BPK. Northern and Western blotting analyses revealed that Aik is expressed highly in testis and various proliferating cells including HeLa cells. In HeLa cells, the endogenous levels of aik mRNA and protein contents are tightly regulated during cell cycle progression. Both of these levels are low in G1/S, accumulate during G2/M, and reduce rapidly after mitosis. Its protein kinase activity is also enhanced at mitosis as inferred by exogenous casein phosphorylation. Immunofluorescence studies using a specific antibody have shown that Aik is localized to the spindle pole during mitosis, especially from prophase through anaphase. These results strongly suggest that Aik is a novel member of a protein kinase family possibly involved in a centrosome function(s) such as chromosome segregation or spindle formation.
The cDNA and derived amino acid sequence for human osteopontin (1989)

Journal Article The cDNA and derived amino acid sequence for human osteopontin Get access Michael C. Kiefer, Michael C. Kiefer Chiron Corporation4560 Horton Street, Emeryville, CA 94680, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Diane M. Bauer, Diane M. Bauer Chiron Corporation4560 Horton Street, Emeryville, CA 94680, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Philip J. Barr Philip J. Barr Chiron Corporation4560 Horton Street, Emeryville, CA 94680, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 17, Issue 8, 25 April 1989, Page 3306, https://doi.org/10.1093/nar/17.8.3306 Published: 25 April 1989 Article history Received: 19 January 1989 Published: 25 April 1989
DNA mismatch repair (MMR) mediates 6-thioguanine genotoxicity by introducing single-strand breaks to signal a G2-M arrest in MMR-proficient RKO cells. (2003)

Our data suggest that MMR mediates 6-TG-induced G(2)-M arrest by introducing SSBs to signal a persistent G(2)-M arrest leading to enhanced cell death.
Primary Care Nursing in Home Healthcare (1994)

Central Coast Visiting Nurse Association in Monterey, California, has a program called Primary Care Nursing that offers many benefits for patients, physicians, and nurses. Patient care in the home setting is enhanced with the continuity provided by the same registered nurse. The patient's physician comes to rely on the knowledge and skills of the primary care nurse in the field, and communication is enhanced. As primary care nurses, registered nurses advance their career opportunities and home care case management skills.
Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex (2002)

Sox-2 is a transcriptional cofactor expressed in embryonic stem (ES) cells as well as in neuronal cells. It has been demonstrated that Sox-2 plays an important role in supporting gene expression in ES cells, especially by forming a complex with embryonic Octamer factor, Oct-3/4. Here, we have analyzed the regulatory regions of the Sox-2 gene and identified two enhancers which stimulate transcription in ES cells as well as in embryonal carcinoma cells. These regulatory regions, which we termed Sox regulatory regions (SRR) 1 and 2, exert their function specifically when cells are in an undifferentiated state. Interestingly, like the regulatory elements of FGF-4 and UTF1 genes, combinatorial action of Octamer and Sox-2 binding sites support the SRR2 activity. However, biochemical analyses reveal that, due to the unique sequence and/or its organization, the SRR2 bears distinct characteristics from those of FGF-4 and UTF1 regulatory elements. That is, unlike the FGF-4 gene enhancer, the SRR2 precludes the binding of the Oct-1-Sox-2 complex. The difference between the SRR2 and UTF1 regulatory element is in the ability of SRR2 to recruit the Oct-6-Sox-2 complex as well as the Oct-3/4-Sox-2 complex. Co-transfection analyses confirm that both complexes are able to stimulate transcription through the SRR2 element.
Chemical communication among bacteria (2003)

Cell-cell communication in bacteria is accomplished through the exchange of chemical signal molecules called autoinducers. This process, called quorum sensing, allows bacteria to monitor their environment for the presence of other bacteria and to respond to fluctuations in the number and/or species present by altering particular behaviors. Most quorum-sensing systems are species- or group-specific, which presumably prevents confusion in mixed-species environments. However, some quorum-sensing circuits control behaviors that involve interactions among bacterial species. These quorum-sensing circuits can involve both intra- and interspecies communication mechanisms. Finally, anti-quorumsensing strategies are present in both bacteria and eukaryotes, and these are apparently designed to combat bacteria that rely on cell-cell communication for the successful adaptation to particular niches.
Nature of the transition state in the oxidation of olefins by chromium(VI) (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNature of the transition state in the oxidation of olefins by chromium(VI)A. K. Awasthy and Jan RocekCite this: J. Am. Chem. Soc. 1969, 91, 4, 991–996Publication Date (Print):February 1, 1969Publication History Published online1 May 2002Published inissue 1 February 1969https://pubs.acs.org/doi/10.1021/ja01032a034https://doi.org/10.1021/ja01032a034research-articleACS PublicationsRequest reuse permissionsArticle Views161Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characterization of Borrelia burgdorferi isolates by restriction endonuclease analysis and DNA hybridization (1989)

Genomes of several Borrelia burgdorferi isolates from North America and Europe were characterized by restriction endonuclease analysis and DNA hybridization using labeled B. burgdorferi whole-cell DNA (strain ATCC 35210). Several different restriction and homology patterns were observed among these isolates, indicating genotypic heterogeneity within this genus and species. It was concluded from this study that restriction endonuclease analysis of B. burgdorferi whole-cell DNA may be a reliable and accurate method for identifying strains or genotypes of the Lyme disease agent.
ATM has a major role in the double-strand break repair pathway dysregulation in sporadic breast carcinomas and is an independent prognostic marker at both mRNA and protein levels (2015)

We confirmed that ATM expression is an independent prognostic marker at both RNA and protein levels. We showed that alteration of ATM is involved in dysregulation of the DSB repair pathway. Finally, miR-203 may be responsible for downregulation of ATM in breast cancers.
[Screening human gastric carcinoma-associated antigens by serologic proteome analysis]. (2007)

Thirteen human gastric carcinoma-associated antigens were found by SERPA. The results provide foundation for screening molecular biomarkers for the diagnosis, therapy, prognostic evaluation, and prevention of human gastric carcinoma.
Species- or isozyme-specific enzyme inhibitors. 4. Design of a two-site inhibitor of adenylate kinase with isozyme selectivity (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpecies- or isozyme-specific enzyme inhibitors. 4. Design of a two-site inhibitor of adenylate kinase with isozyme selectivityAlexander Hampton, Francis Kappler, and Donald PickerCite this: J. Med. Chem. 1982, 25, 6, 638–644Publication Date (Print):June 1, 1982Publication History Published online1 May 2002Published inissue 1 June 1982https://pubs.acs.org/doi/10.1021/jm00348a006https://doi.org/10.1021/jm00348a006research-articleACS PublicationsRequest reuse permissionsArticle Views224Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Classification of chitinases modules (1999)

Chitinases frequently display a modular structure featuring a catalytic domain attached to one or several ancillary noncatalytic domains whose function is often chitin binding. Gene cloning and DNA sequencing have allowed the determination of a massive number of amino acid sequences of chitinases during the last 10 years. This chapter presents a unifying classification system of the various chitinase modules that combines specific features of their sequences, three-dimensional structures and reaction mechanisms.
Cloning of two loci for synapse protein Snap25 in zebrafish: Comparison of paralogous linkage groups suggests loss of one locus in the mammalian lineage (1998)

Synaptosome-associated protein of 25 kDa (Snap25) is an intracellular protein that is defined as a target receptor for synapse vesicles prior to neurotransmitter release. Snap25 is highly conserved, with 61% identity between human and Drosophila melanogaster. Whereas mammals and chicken have a single locus for Snap25, the tetraploid goldfish has at least three loci. We report that the zebrafish has two loci with 91% amino acid identity to each other. The alternative splicing of exon 5 arose before the gene duplication. The expression patterns of the two loci are virtually identical in adult zebrafish. The two zebrafish snap25 loci are located in paralogous linkage groups that seem to correspond to human chromosome 20, which harbors the SNAP locus, and human chromosome 14. Because no additional Snap25 homologue has been reported for any mammal or chicken, snap25.2 may have been lost in the amniote or even tetrapod lineage.
Correction of a deletion mutant by gene targeting with an adenovirus vector. (1993)

The usefulness of adenovirus type 5 as a vector for homologous recombination was examined in CHO cells by using the adenine phosphoribosyltransferase (aprt) gene. Infection of a hemizygous CHO APRT- cell line containing a 3-bp deletion in exon 5 of the aprt gene with a recombinant adenovirus containing the wild-type gene resulted in restoration of the APRT+ phenotype at a frequency of 10(-5) to 10(-6) per infected cell. A relatively high frequency (approximately 6 to 20%) of the transductants appears to result from a homologous recombination event. The mutation on the chromosomal aprt gene is corrected in the homologous recombinants, and APRT expression is restored to a normal hemizygous level. Neither adenovirus nor exogenous promoter sequences are detected in the homologous recombinants. The remaining transductants result from random integration of the aprt gene with the adenovirus sequence. A number of adenovirus vectors containing different promoter sequences linked to the hamster aprt gene were constructed. A possible role for the promoter region in the homologous recombination event was indicated by the lack of homologous recombination in constructs lacking an active promoter.
Switchable Tackiness and Wettability of a Liquid Crystalline Polymer (1999)

The spreading velocity of liquids on the surface of a liquid crystalline polymer can be tremendously affected by a slight temperature change. Indeed, a bulk transition between a highly ordered smectic and an isotropic phase induces a sharp change from a rigid to a soft behavior, with consequent effects on the tack properties of the liquid crystalline polymer and on the dewetting dynamics of a liquid on its surface.
Accelerated displacement of duplex DNA strands by a synthetic circular oligodeoxynucleotide (1993)

T. A. Perkins, J. L. Goodman and E. T. Kool, J. Chem. Soc., Chem. Commun., 1993, 215 DOI: 10.1039/C39930000215
Increased Aortic Root Size is Associated with Nocturnal Hypoxia and Diastolic Blood Pressure in Obstructive Sleep Apnea (2011)

In OSA patients, nocturnal hypoxemia decreased BRS and increased diastolic BP, which was the main factor influencing aortic root size.
PubLayNet: largest dataset ever for document layout analysis (2019)

Recognizing the layout of unstructured digital documents is an important step when parsing the documents into structured machine-readable format for downstream applications. Deep neural networks that are developed for computer vision have been proven to be an effective method to analyze layout of document images. However, document layout datasets that are currently publicly available are several magnitudes smaller than established computing vision datasets. Models have to be trained by transfer learning from a base model that is pre-trained on a traditional computer vision dataset. In this paper, we develop the PubLayNet dataset for document layout analysis by automatically matching the XML representations and the content of over 1 million PDF articles that are publicly available on PubMed Central. The size of the dataset is comparable to established computer vision datasets, containing over 360 thousand document images, where typical document layout elements are annotated. The experiments demonstrate that deep neural networks trained on PubLayNet accurately recognize the layout of scientific articles. The pre-trained models are also a more effective base mode for transfer learning on a different document domain. We release the dataset (https://github.com/ibm-aur-nlp/PubLayNet) to support development and evaluation of more advanced models for document layout analysis.
One-step Needle Pose Estimation for Ultrasound Guided Biopsies (2007)

A pose estimation method is proposed for measuring the position and orientation of a needle. The technique is to be used as a touchless needle guide system for guidance of percutaneous procedures with 4D ultrasound. A pair of uncalibrated, light-weight USB cameras is used as inputs. A database is prepared offline, from the needle line estimated from camera-captured images and from the true needle line recorded from an independent tracking device. A non-parametric learning algorithm determines the best fit model from the database. This model can then be used in real-time to estimate the true position of the needle with inputs from only the camera images. Simulation results confirm the feasibility of the method and show how a small, accurately made database can provide satisfactory results. In a series of tests with cameras, we achieved an average error of 2.4mm in position and 2.61 degrees in orientation. A study of the results shows that the overall accuracy of the method is affected by the system designed to make the database.
Anisomycin activates JNK and sensitises DU 145 prostate carcinoma cells to Fas mediated apoptosis (2002)

Treatment of the hormone refractory prostate cancer cell line DU 145 with sublethal concentrations of chemotherapeutic drugs has been reported to sensitise these cells to Fas mediated apoptosis. However, the mechanism by which this occurs has not been determined. Our group has shown that inhibition of JNK activity completely abrogates the effects of chemotherapeutic drugs. Using anisomycin, a potent JNK agonist, we have demonstrated a role for JNK in Fas mediated apoptosis in DU 145 cells. Inhibition of Caspase 8 and Caspase 9 completely inhibits this process which suggests that DU 145 cells require mitochondrial amplification of the Fas apoptotic signal. Furthermore, we have shown that inhibition of Fas mediated apoptosis is an early event in DU 145 cells, occurring upstream of Caspase 8 cleavage. It is hoped that identifying the target of JNK will allow novel therapies to be developed for the treatment of hormone refractory prostate cancer. Such therapies are especially important because no single or combined treatment to date has significantly prolonged survival in patients with hormone refractory prostate cancer.
Preimplantation diagnosis by whole-genome amplification, PCR amplification, and solid-phase minisequencing of blastomere DNA. (1996)

We have developed a new method for preimplantation diagnosis of inherited diseases. Our procedure for the identification of point mutations in single cells combines whole-genome amplification using 15-mer random primers (primer extension preamplification, PEP) with a single locus-specific PCR amplification, followed by detection of the mutation by solid-phase minisequencing. The procedure was evaluated by detecting three disease-causing mutations and seven polymorphic nucleotides located on different human chromosomes from single granuloma and blastomere cells. The correct genotype of the cell was identified at 96% of the nucleotide positions analyzed, showing that a representative part of the genome is amplified during PEP. We estimate that PEP yielded at least 1000 copies of the genome. The quantitative nature of the solid-phase minisequencing method allowed us to notice that preferential amplification of one allele occurs at heterozygous loci during PEP, which is a potential problem in preimplantation diagnosis.
A New Secure Architecture of Network Computer Based on Single CPU and Dual Bus (2008)

Internet is facing a serious problem: open and security. Private data, such as credit card information, login account and password, and etc., is easily theft by hackers. In this paper, we propose a new secure architecture of network computer (SANC). This new architecture is aimed to build a kind of low-cost intrusion-free network computer and reduce vulnerability of Neumann model. It is comprised of one CPU and two physically isolated system buses, secure and non-secure. Only non-secure bus has Internet interfaces. Devices, storing private data, can only be accessed through secure bus. Internet related applications, only running on the non-secure bus, have no opportunity to access resources on the secure bus. Besides, only one CPU can efficiently control the cost. To control the connection between CPU and two system buses, and ensure only one bus can be connected to CPU at the same time, a bus bridge (BB) is designed.
A Reexamination of Active and Passive Tumor Targeting by Using Rod-Shaped Gold Nanocrystals and Covalently Conjugated Peptide Ligands (2010)

The targeted delivery of nanoparticles to solid tumors is one of the most important and challenging problems in cancer nanomedicine, but the detailed delivery mechanisms and design principles are still not well understood. Here we report quantitative tumor uptake studies for a class of elongated gold nanocrystals (called nanorods) that are covalently conjugated to tumor-targeting peptides. A major advantage in using gold as a "tracer" is that the accumulated gold in tumors and other organs can be quantitatively determined by elemental mass spectrometry (gold is not a natural element found in animals). Thus, colloidal gold nanorods are stabilized with a layer of polyethylene glycols (PEGs) and are conjugated to three different ligands: (i) a single-chain variable fragment (ScFv) peptide that recognizes the epidermal growth factor receptor (EGFR); (ii) an amino terminal fragment (ATF) peptide that recognizes the urokinase plasminogen activator receptor (uPAR); and (iii) a cyclic RGD peptide that recognizes the a(v)β(3) integrin receptor. Quantitative pharmacokinetic and biodistribution data show that these targeting ligands only marginally improve the total gold accumulation in xenograft tumor models in comparison with nontargeted controls, but their use could greatly alter the intracellular and extracellular nanoparticle distributions. When the gold nanorods are administered via intravenous injection, we also find that active molecular targeting of the tumor microenvironments (e.g., fibroblasts, macrophages, and vasculatures) does not significantly influence the tumor nanoparticle uptake. These results suggest that for photothermal cancer therapy, the preferred route of gold nanorod administration is intratumoral injection instead of intravenous injection.