gebhart/patrepeval huggingface dataset:
Kernel Regression for Image Processing and Reconstruction (2007)

In this paper, we make contact with the field of nonparametric statistics and present a development and generalization of tools and results for use in image processing and reconstruction. In particular, we adapt and expand kernel regression ideas for use in image denoising, upscaling, interpolation, fusion, and more. Furthermore, we establish key relationships with some popular existing methods and show how several of these algorithms, including the recently popularized bilateral filter, are special cases of the proposed framework. The resulting algorithms and analyses are amply illustrated with practical examples.
Pre‐activation of retinoid signaling facilitates neuronal differentiation of mesenchymal stem cells (2010)

Mesenchymal stem cells (MSCs) can differentiate into neurons in an appropriate cellular environment. Retinoid signaling pathway is required in neural development. However, the effect and mechanism through retinoid signaling regulates neuronal differentiation of MSCs are still poorly understood. Here, we report that all-trans-retinoic acid (ATRA) pre-induction improved neuronal differentiation of rat MSCs. We found that, when MSCs were exposed to different concentrations of ATRA (0.01-100 micromol/L) for 24 h and then cultured with modified neuronal induction medium (MNM), 1 micromol/L ATRA pre-induction significantly improved neuronal differentiation efficiency and neural-cell survival. Compared with MNM alone induced neural-like cells, ATRA/MNM induced cells expressed higher levels of Nestin, neuron specific enolase (NSE), microtubule-associated protein-2 (MAP-2), but lower levels of CD68, glial fibrillary acidic protein (GFAP), and glial cell line-derived neurotrophic factor(GDNF), also exhibited higher resting membrane potential and intracellular calcium concentration, supporting that ATRA pre-induction promotes maturation and function of derived neurons but not neuroglia cells from MSCs. Endogenous retinoid X receptors (RXR) RXRalpha and RXRgamma (and to a lesser extent, RXRbeta) were weakly expressed in MSCs. But the expression of RARalpha and RARgamma was readily detectable, whereas RARbeta was undetectable. However, at 24 h after ATRA treatment, the expression of RARbeta, not RARalpha or RARgamma, increased significantly. We further found the subnuclear redistribution of RARbeta in differentiated neurons, suggesting that RARbeta may function as a major mediator of retinoid signaling during neuronal differentiation from MSCs. ATRA treatment upregulated the expression of Vimentin and Stra13, while it downregulated the expression of Brachyury in MSCs. Thus, our results demonstrate that pre-activation of retinoid signaling by ATRA facilitates neuronal differentiation of MSCs.
Effects of Near-Infrared Laser on Neural Cell Activity (2004)

Near‐infrared laser has been used to relieve patients from various kinds of pain caused by postherpetic neuralgesia, myofascial dysfunction, surgical and traumatic wound, cancer, and rheumatoid arthritis. Clinically, He‐Ne (λ=632.8 nm, 780 nm) and Ga‐Al‐As (805 ± 25 nm) lasers are used to irradiate trigger points or nerve ganglion. However the precise mechanisms of such biological actions of the laser have not yet been resolved. Since laser therapy is often effective to suppress the pain caused by hyperactive excitation of sensory neurons, interactions with laser light and neural cells are suggested. As neural excitation requires large amount of energy liberated from adenosine triphosphate (ATP), we examined the effect of 830‐nm laser irradiation on the energy metabolism of the rat central nervous system and isolated mitochondria from brain. The diode laser was applied for 15 min with irradiance of 4.8 W/cm2 on a 2 mm‐diameter spot at the brain surface. Tissue ATP content of the irradiated area in the cerebral cortex was 19 % higher than that of the non‐treated area (opposite side of the cortex), whereas the ADP content showed no significant difference. Irradiation at another wavelength (652 nm) had no effect on either ATP or ADP contents. The temperature of the brain tissue was increased 4.5 – 5.0 °C during the irradiation of both 830‐nm and 652‐nm laser light. Direct irradiation of the mitochondrial suspension did not show any wavelength‐dependent acceleration of respiration rate nor ATP synthesis. These results suggest that the increase in tissue ATP content did not result from the thermal effect, but from specific effect of the laser operated at 830 nm. Electrophysiological studies showed the hyperpolarization of membrane potential of isolated neurons and decrease in membrane resistance with irradiation of the laser, suggesting an activation of potassium channels. Intracellular ATP is reported to regulate some kinds of potassium channels. Possible mechanisms of laser effect on neural activity through interaction between ATP and potassium channels will be discussed.
Fast slice‐selective radio‐frequency excitation pulses for mitigating B inhomogeneity in the human brain at 7 Tesla (2008)

A novel radio-frequency (RF) pulse design algorithm is presented that generates fast slice-selective excitation pulses that mitigate B+1 inhomogeneity present in the human brain at high field. The method is provided an estimate of the B+1 field in an axial slice of the brain and then optimizes the placement of sinc-like "spokes" in kz via an L1-norm penalty on candidate (kx, ky) locations; an RF pulse and gradients are then designed based on these weighted points. Mitigation pulses are designed and demonstrated at 7T in a head-shaped water phantom and the brain; in each case, the pulses mitigate a significantly nonuniform transmit profile and produce nearly uniform flip angles across the field of excitation (FOX). The main contribution of this work, the sparsity-enforced spoke placement and pulse design algorithm, is derived for conventional single-channel excitation systems and applied in the brain at 7T, but readily extends to lower field systems, nonbrain applications, and multichannel parallel excitation arrays.
Clustered Genomic Alterations in Chromosome 7p Dictate Outcomes and Targeted Treatment Responses of Lung Adenocarcinoma WithEGFR-Activating Mutations (2011)

Our results shed light on why responses to EGFR-TKIs are heterogeneous among patients with EGFR-activating mutation. They may lead to better patient management in this population.
Dilemmas in the Management of Atrial Fibrillation in Chronic Kidney Disease (2008)

Patients with chronic kidney disease (CKD) have an increased risk for cardiovascular morbidity and mortality. Little attention has been paid to the problem of atrial fibrillation, although this arrhythmia is very frequent with a prevalence of 13 to 27% in patients on long-term hemodialysis. Because of the large number of pathophysiologic mechanisms involved, these patients have a high risk for both thromboembolic events and hemorrhagic complications. Stroke is a frequent complication in CKD: The US Renal Data System reports an incidence of 15.1% in hemodialysis patients compared with 9.6% in patients with other stages of CKD and 2.6% in a control cohort without CKD. The 2-yr mortality rates after stroke in these subgroups were 74, 55, and 28%, respectively. Although oral coumadin is the treatment of choice for atrial fibrillation, its use in patients with CKD is reported only in limited studies, all in hemodialysis patients, and is associated with a markedly increased rate of bleeding compared with patients without CKD. With regard to the high risk for stroke and the conflicting data about oral anticoagulation, an individualized stratification algorithm is presented based on relevant studies.
Wavelength-tunable add/drop optical filter (1996)

A new type of add/drop optical filter discretely tunable along equally spaced channels is presented. This device may have very useful applications in WDM optical networks.
Plasma membrane association of three classes of bacterial toxins is mediated by a basic-hydrophobic motif (2011)

Plasma membrane targeting is essential for the proper function of many bacterial toxins. A conserved fourhelical bundle membrane localization domain (4HBM) was recently identified within three diverse families of toxins: clostridial glucosylating toxins, MARTX toxins and Pasteurella multocida-like toxins. When expressed in tissue culture cells or in yeast, GFP fusions to at least one 4HBM from each toxin family show significant peripheral membrane localization but with differing profiles. Both in vivo expression and in vitro binding studies reveal that the ability of these domains to localize to the plasma membrane and bind negatively charged phospholipids requires a basic-hydrophobic motif formed by the L1 and L3 loops. The different binding capacity of each 4HBM is defined by the hydrophobicity of an exposed residue within the motif. This study establishes that bacterial effectors utilize a normal host cell mechanism to locate the plasma membrane where they can then access their intracellular targets.
Electrophysiological Effects of 4-Hydroxynonenal, an Aldehydic Product of Lipid Peroxidation, on Isolated Rat Ventricular Myocytes (1995)

Aldehydic products of lipid peroxidation, such as 4-hydroxynonenal (4-HNE), have been implicated in the etiology of pathological changes under oxidative stress. To identify the mechanism by which 4-HNE alters cellular excitability, its effects on isolated rat ventricular myocytes were studied. Superfusion with 100 to 880 mumol/L 4-HNE led to a time- and concentration-dependent rigor shortening of myocytes. A reduction in [Ca2+]o and inhibition of transsarcolemmal Ca2+ transport by 1 mmol/L La3+ did not affect either the magnitude or the time course of 4-HNE-induced myocyte rigor. Superfusion of myocytes with 400 mumol/L 4-HNE led to an increase in the action potential duration, progressive depolarization of the resting membrane potential, and an increase in the input resistance (Rin) of the myocyte (phase I), followed by a loss of electrical excitability. Continued superfusion with 4-HNE resulted in membrane hyperpolarization and a prominent decrease in the Rin (phase II). The decrease in Rin coincided with myocyte rigor. In whole-cell voltage-clamp experiments, superfusion with 4-HNE inhibited current through the inward rectifier K+ channel (IK1). 4-HNE had no effect on either the magnitude or the rate of "rundown" of L-type Ca2+ currents. Exposure to 4-HNE led to an increase in the magnitude of the fast inward Na+ current (INa). The voltage dependence of the steady state parameters for activation and inactivation of INa shifted to more positive potentials, with a resultant increase in the window current. 4-HNE-induced myocyte rigor was accompanied by a large increase in time-independent currents that displayed linear dependence on the membrane potential and were inhibited by glibenclamide, suggesting activation of the ATP-sensitive K+ channel. Steady state currents recorded in Cs(+)-containing Ringer's solution with La3+ and tetrodotoxin and Cs(+)-containing internal solution (leak currents) were not affected by 4-HNE. Superfusion with 4-HNE resulted in a significant decrease in the cellular concentration of nonprotein thiols and a severe decrease in [ATP]i. The energy charge of the myocytes fell from 0.9 to 0.3. These observations indicate that 4-HNE-induced membrane depolarization may be due to an inhibition of IK1. Changes in voltage dependence of INa, inhibition of IK1, and membrane depolarization appear to contribute to the prolongation of the action potential, observed during phase I. Depletion of [ATP]i may be responsible for changes observed during phase II, ie, activation of the ATP-sensitive K+ channels, membrane hyperpolarization, decrease in Rin, and rigor shortening of the myocytes. These results suggest that stable products of lipid peroxidation, such as 4-HNE, are proarrhythmic and may contribute to the cytotoxic effects of oxidative stress.
PCSK9: a convertase that coordinates LDL catabolism (2008)

The identification and characterization of proprotein convertase subtilisin-like/kexin type 9 (PCSK9) have provided new insights into LDL metabolism and the causal role of LDL in coronary heart disease (CHD). PCSK9 is a secreted protease that mediates degradation of the LDL receptor by interacting with the extracellular domain and targeting the receptor for degradation. Individuals with loss-of-function mutations in PCSK9 have reduced plasma levels of LDL cholesterol and are protected from CHD; these observations have validated PCSK9 as a therapeutic target and suggested new approaches for the treatment and prevention of CHD.
CloudAV: N-version antivirus in the network cloud (2008)

Antivirus software is one of the most widely used tools for detecting and stopping malicious and unwanted files. However, the long term effectiveness of traditional host-based antivirus is questionable. Antivirus software fails to detect many modern threats and its increasing com-plexity has resulted in vulnerabilities that are being ex-ploited by malware. This paper advocates a new model for malware detection on end hosts based on providing antivirus as an in-cloud network service. This model en-ables identification of malicious and unwanted software by multiple, heterogeneous detection engines in paral-lel, a technique we term ‘N-version protection’. This approach provides several important benefits including better detection of malicious software, enhanced foren-sics capabilities, retrospective detection, and improved deployability and management. To explore this idea we construct and deploy a production quality in-cloud an-tivirus system called CloudAV. CloudAV includes a lightweight, cross-platform host agent and a network ser-vice with ten antivirus engines and two behavioral detec-tion engines. We evaluate the performance, scalability, and efficacy of the system using data from a real-world deployment lasting more than six months and a database of 7220 malware samples covering a one year period. Using this dataset we find that CloudAV provides 35% better detection coverage against recent threats compared to a single antivirus engine and a 98 % detection rate across the full dataset. We show that the average length of time to detect new threats by an antivirus engine is 48 days and that retrospective detection can greatly mini-mize the impact of this delay. Finally, we relate two case studies demonstrating how the forensics capabilities of CloudAV were used by operators during the deployment. 1
Activated Androgen Receptor Downregulates E-Cadherin Gene Expression and Promotes Tumor Metastasis (2008)

The loss of E-cadherin gene expression can cause the dysfunction of the cell-cell junction to trigger tumor metastasis. Members of the Snail family of transcription factors are repressors of the expression of the E-cadherin gene. In this study, we showed that the activated androgen receptor (AR) is a novel repressor of E-cadherin gene expression and can promote metastasis. Our results demonstrated that the activated AR could bind to the E-cadherin promoter in vitro and in vivo. The activated AR and HDAC1 had synergistic effects in downregulating E-cadherin gene expression. Treating cells with the AR ligand, dihydrotestosterone (DHT), triggered the reduction of E-cadherin expression and induced changes in cell morphology from an epithelial-like to a mesenchymal-like appearance. When nonmetastatic breast cancer cells expressing cytoplasmic AR were transplanted into mice and the mice were treated with DHT, tumors were detected at metastatic sites, whereas no tumors were detected in transplanted mice without DHT treatment. Furthermore, clinical data from breast cancer patients with invasive ductal carcinomas showed high levels of AR expression in the nuclei and low levels of E-cadherin expression. These results suggest that, similarly to Snail and Twist, the activated AR can downregulate E-cadherin expression to promote the activation of epithelial-mesenchymal transition and tumor metastasis.
True number portability and advanced call screening in a SIP-based IP telephony system (1999)

Custom local area signaling service features offered in the PSTN have certain limitations due to the closed nature of PSTN network signaling. The adoption of telephony over IP (IP telephony) will enable a new paradigm of services and features that are not possible to implement in today's PSTN. This is especially the case for services that make use of personal, trusted information, which can be provided by a user's personal digital assistant. We demonstrate how personal information can be coupled with an IP telephony service to provide user-customized call handling by the network. In particular, we describe a demonstration architecture that includes Ethernet-attached phones running SIP, with an interface to synchronize with PDAs that supply personal information. The proposed architecture is quite flexible; it can support enhanced versions of the current PSTN and private branch exchange services, in addition to many new features and services. We describe true number portability and advanced call screening as examples of new services in a hybrid PSTN/IP telephony environment.
CD70/CD27 signaling promotes blast stemness and is a viable therapeutic target in acute myeloid leukemia (2016)

Aberrant proliferation, symmetric self-renewal, increased survival, and defective differentiation of malignant blasts are key oncogenic drivers in acute myeloid leukemia (AML). Stem cell gene signatures predict poor prognosis in AML patients; however, with few exceptions, these deregulated molecular pathways cannot be targeted therapeutically. In this study, we demonstrate that the TNF superfamily ligand-receptor pair CD70/CD27 is expressed on AML blasts and AML stem/progenitor cells. CD70/CD27 signaling in AML cells activates stem cell gene expression programs, including the Wnt pathway, and promotes symmetric cell divisions and proliferation. Soluble CD27, reflecting the extent of CD70/CD27 interactions in vivo, was significantly elevated in the sera of newly diagnosed AML patients and is a strong independent negative prognostic biomarker for overall survival. Blocking the CD70/CD27 interaction by mAb induced asymmetric cell divisions and differentiation in AML blasts and AML stem/progenitor cells, inhibited cell growth and colony formation, and significantly prolonged survival in murine AML xenografts. Importantly, hematopoietic stem/progenitor cells from healthy BM donors express neither CD70 nor CD27 and were unaffected by blocking mAb treatment. Therefore, targeting CD70/CD27 signaling represents a promising therapeutic strategy for AML.
Efficient differentiation and function of human macrophages in humanized CSF-1 mice (2011)

Humanized mouse models are useful tools to understand pathophysiology and to develop therapies for human diseases. While significant progress has been made in generating immunocompromised mice with a human hematopoietic system, there are still several shortcomings, one of which is poor human myelopoiesis. Here, we report that human CSF-1 knockin mice show augmented frequencies and functions of human myeloid cells. Insertion of human CSF1 into the corresponding mouse locus of Balb/c Rag2(-/-) γc(-/-) mice through VELOCIGENE technology resulted in faithful expression of human CSF-1 in these mice both qualitatively and quantitatively. Intra-hepatic transfer of human fetal liver derived hematopoietic stem and progenitor cells (CD34(+)) in humanized CSF-1 (CSF1(h/h)) newborn mice resulted in more efficient differentiation and enhanced frequencies of human monocytes/macrophages in the bone marrow, spleens, peripheral blood, lungs, liver and peritoneal cavity. Human monocytes/macrophages obtained from the humanized CSF-1 mice show augmented functional properties including migration, phagocytosis, activation and responses to LPS. Thus, humanized mice engineered to express human cytokines will significantly help to overcome the current technical challenges in the field. In addition, humanized CSF-1 mice will be a valuable experimental model to study human myeloid cell biology.
Structural studies of tetracyclines. Crystal and molecular structures of anhydrotetracycline hydrobromide monohydrate and 6-demethyl-7-chlorotetracycline hydrochloride trihydrate (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural studies of tetracyclines. Crystal and molecular structures of anhydrotetracycline hydrobromide monohydrate and 6-demethyl-7-chlorotetracycline hydrochloride trihydrateGus J. Palenik, M. Mathew, and R. RestivoCite this: J. Am. Chem. Soc. 1978, 100, 14, 4458–4464Publication Date (Print):July 1, 1978Publication History Published online1 May 2002Published inissue 1 July 1978https://pubs.acs.org/doi/10.1021/ja00482a024https://doi.org/10.1021/ja00482a024research-articleACS PublicationsRequest reuse permissionsArticle Views140Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A General Natural-language Text Processor for Clinical Radiology (1994)

Without training specific to the four diseases, recall and precision of the system (combined effect of the processor and query generator) were 70% and 87%. Training of the query component increased recall to 85% without changing precision.
Effects of silymarin and pentoxifylline on matrix metalloproteinase-1 and -2 expression and apoptosis in experimental hepatic fibrosis (2008)

Silymarin and PTX were associated with lower histopathologic liver damage, hepatocyte apoptosis, and regulation of extracellular matrix proteins. Lipid peroxidation in hepatocytes was significantly lower in the silymarin group compared with the PTX group. Silymarin and PTX appeared to have hepatoprotective effects in this experimental liver fibrosis model, but further clinical and experimental studies are needed.
A new contactless smartcard IC using an on-chip antenna and an asynchronous micro-controller (2000)

A new generation of Contactless Smartcard Chip is described. The Contactless Smartcard Chip integrates an on-chip coil connected to a power reception system and an emitter/receiver module compatible with the ISO 14443 standard, together with an asynchronous Quasi Delay Insensitive (QDI) 8-bit micro-controller. Beyond the contactless smartcard application field, this new chip demonstrates that System-on-Chip integrating power reception and management, radio-frequency communication and signal processing are feasible. The chip associates analog/digital parts as well as synchronous/asynchronous logics and has been fabricated in a CMOS 6 metal layers .25µm technology from STMicroelectronics.