gebhart/patrepeval huggingface dataset:
Cigarette smoking: implications for psychiatric illness (1993)

Psychiatry has been essentially uninterested in cigarette smoking and nicotine. However, it is the view of this author that both cigarette smoking and smoking cessation are highly relevant to the clinical psychiatrist in the care of patients and that they are potentially a source of important insights into psychopathology. To support that view, the author reviews the evidence that both major depression and depressive symptoms are associated with a high rate of cigarette smoking and that lifetime history of major depression has an adverse impact on smoking cessation. He also reviews the data available on the influence of cigarette smoking cessation on the course of major depression, the relationship between cigarette smoking and other psychiatric diagnoses, particularly schizophrenia, and the neuropharmacology that might underlie these associations. Finally, the implications of these relationships for psychiatry are discussed.
Disubstituted Indazoles as Potent Antagonists of the Integrin αvβ3 (1999)

A new series of indazole-containing alpha(v)beta(3) integrin antagonists is described. Starting with lead compound 18a, variations in a number of structural features were explored with respect to inhibition of the binding of beta(3)-transfected 293 cells to fibrinogen and to selectivity for alpha(v)beta(3) over GPIIbIIIa, another RGD-binding integrin. Indazoles attached to a 2-aminopyridine or 2-aminoimidazole by a propylene linker at the indazole 1-position and to a diaminopropionate derivative via a 5-carboxylate amide provided the best potency with moderate selectivity. Several differences in the SAR of the diaminopropionate moiety were observed between this series and a series of isoxazoline-based selective GPIIbIIIa antagonists. Compound 34a (SM256) was a potent antagonist of alpha(v)beta(3) (IC(50) 2.3 nM) with 9-fold selectivity over GPIIbIIIa.
Cryopreservation of Mature Human Adipocytes (2005)

Cryopreservation of fat grafts for autologous fat transplantation renders repeated harvesting procedures unnecessary. Anecdotic reports have been published, yet data about adipocyte survival are sparse. The beneficial effect of added cryoprotective agents (CPA) is known from other tissues but has not been investigated in adipocytes. Fat cells were harvested using the Coleman method (n = 24). Tests were done after 0, 2, 7, 14, and 30 days of cooling to -20 degrees C and -80 degrees C and after addition of various CPA. Analysis included cell stains, measurement of metabolic activity by MTT and XTT tests, and measurement of cell stability by assessment of extracellular glycerol-3-phosphate-dehydrogenase enzyme. After freezing, up to 92.7% of metabolic activity was lost, but the addition of CPA led to preservation of up to 54% of baseline activity. Also, lower storage temperature showed more cell destruction but yielded higher viability of the surviving cells. Our results implicate that the widely used practice of simple storage in a freezer leads to reinjection of nonviable tissue. Cell survival can be improved by addition of CPA.
Recent advances in arginine metabolism: roles and regulation of the arginases (2009)

As arginine can serve as precursor to a wide range of compounds, including nitric oxide, creatine, urea, polyamines, proline, glutamate and agmatine, there is considerable interest in elucidating mechanisms underlying regulation of its metabolism. It is now becoming apparent that the two isoforms of arginase in mammals play key roles in regulation of most aspects of arginine metabolism in health and disease. In particular, work over the past several years has focused on the roles and regulation of the arginases in vascular disease, pulmonary disease, infectious disease, immune cell function and cancer. As most of these topics have been considered in recent review articles, this review will focus more closely on results of recent studies on expression of the arginases in endothelial and vascular smooth muscle cells, post-translational modulation of arginase activity and applications of arginase inhibitors in vivo.
Molecular characterization of two functional domains of CLIP-170 in vivo (1994)

CLIP-170 is a microtubule-binding protein isolated from HeLa cells that is involved in the interaction of endosomes with microtubules. The basic N-terminal domain of CLIP-170 binds to microtubules in vitro. To characterize further the functional domains of this cytoplasmic linker protein, we have transiently expressed intact and mutant forms of CLIP-170 in mammalian cells (HeLa and Vero cells) and show that the tandem repeat present in the N-terminal domain is essential for its binding to microtubules in vivo as previously found in vitro. With increasing levels of expression of CLIP-170, the sites with which the peripheral ends of microtubules interact enlarge, eventually forming large patches, which finally lead to the apparent bundling of microtubules. These patches do not form when the C-terminal domain is absent from the transfected protein. Modification of the microtubule-binding region, particularly of the tandem repeat motif, modulates the binding of CLIP-170 to microtubules. Overexpressed CLIP-170 appears neither to interact with nor to influence the organization of the intermediate filaments, and collapsing the network of intermediate filaments with microinjected antibodies against vimentin has no effect on the distribution of CLIP-170. These data suggest that CLIP-170 has at least two functional domains in vivo, an N-terminal microtubule-binding domain, and a C-terminal domain that is involved in the anchoring of microtubules to peripheral cytoplasmic structures.
Matching images with different resolutions (2002)

In this paper we address the problem of matching two images with two different resolutions: a high-resolution image and a low-resolution one. On the premise that changes in resolution act as a smoothing equivalent to changes in scale, a scale-space representation of the high-resolution image is produced. Hence the one-to-one classical image matching paradigm becomes one-to-many because the low-resolution image is compared with all the scale-space representations of the high-resolution one. Key to the success of such a process is the proper representation of the features to be matched in scale-space. We show how to extract interest points at variable scales and we devise a method allowing the comparison of two images at two different resolutions. The method comprises the use of photometric- and rotation-invariant descriptors, a geometric model mapping the high-resolution image onto a low-resolution image region, and an image matching strategy based on the robust estimation of this geometric model. Extensive experiments show that our matching method can be used for scale changes up to a factor 6.
Astronomical Fourier spectrometer (1975)

A high resolution near ir Fourier spectrometer with the same general design as previously described laboratory instruments has been built for astronomical observations at a coudé focus. Present spectral range is 0.8-3.5 microm with PbS and Ge detectors and maximum path difference 1 m. The servo system can accommodate various recording modes: stepping or continuous scan, path difference modulation, sky chopping. A real time computer is incorporated into the system, which has been set up at the Hale 500-cm telescope on Mount Palomar. Samples of the results are given.
ERp44 Mediates a Thiol-independent Retention of Formylglycine-generating Enzyme in the Endoplasmic Reticulum (2008)

Inside the endoplasmic reticulum (ER) formylglycine-generating enzyme (FGE) catalyzes in newly synthesized sulfatases the post-translational oxidation of a specific cysteine. Thereby formylglycine is generated, which is essential for sulfatase activity. Here we show that ERp44 interacts with FGE forming heterodimeric and, to a lesser extent, also heterotetrameric and octameric complexes, which are stabilized through disulfide bonding between cysteine 29 of ERp44 and cysteines 50 and 52 in the N-terminal region of FGE. ERp44 mediates FGE retrieval to the ER via its C-terminal RDEL signal. Increasing ERp44 levels by overexpression enhances and decreasing ERp44 levels by silencing reduces ER retention of FGE. Suppressing disulfide bonding by mutating the critical cysteines neither abrogates ERp44.FGE complex formation nor interferes with ERp44-mediated retention of FGE, indicating that noncovalent interactions between ERp44 and FGE are sufficient to mediate ER retention. The N-terminal region of FGE harboring Cys(50) and Cys(52) is dispensible for catalytic activity in vitro but required for FGE-mediated activation of sulfatases in vivo. This in vivo activity is affected neither by overexpression nor by silencing of ERp44, indicating that a further ER component interacting with the N-terminal extension of FGE is critical for sulfatase activation.
Separation of 100-Kilobase DNA Molecules in 10 Seconds (2001)

Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and lambda (48.5 kbp) DNAs could be resolved into two clearly separated bands in approximately 10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.
Reconstruction of degenerate rd mouse retina by transplantation of transgenic photoreceptors. (1992)

Photoreceptors from neonatal transgenic mice with normally developing retinas were transplanted to the subretinal spaces of 2-3-month-old rd mutant mice that lack photoreceptors. The transgenic mouse photoreceptors express high levels of the lac Z reporter gene product, beta-galactosidase, which facilitated tracking the transplanted cells. Two sources were used for these cells: (1) dissection of retinal microaggregates containing photoreceptors and (2) papain-dissociated photoreceptors. Host retinas were examined after transplantation. Both methods led to survival of photoreceptors for at least 2 mo after transplantation. Relatively mature outer segments were found only in transplanted microaggregates; this occurred optimally when the cells were adjacent to the retinal pigment epithelium (RPE). beta-galactosidase-labeled outer segments associated closely with the apical processes of the host RPE, which, together with labeled phagosomes in the RPE cells, suggested functional interaction between the transplanted photoreceptors and the host RPE. This study is the first to the authors' knowledge to show electron microscopically that a morphologically normal-appearing photoreceptor layer can be reconstructed in an otherwise photoreceptorless retina.
Trypsin Inhibitor Activity in Commercial Soybean Products in Japan. (1997)

The effect of trypsin inhibitor (TI) in soybean on the prevention of cancer has been reported in animal studies. However, the actual intake of active TI in daily life is not known. In this experiment we measured TI activity in various soybean products and the effects of some factors of processing on the TI activity. Average TI activity in whole soybean was 4,819 U/100 g, and the average percentages of remaining activity in products against that of whole soybean were momen-tofu 2.5%, yose-tofu 3.4%, kinugoshi-tofu 4.3%, jyuten-tofu 7.9%, soymilk 13.0%, natto 0.7%, soy sauce 0.8% and miso 0.3%. By heating soymilk at 100 degrees C, TI activity decreased to 11% at 10 min and 5% at 20 min. After heating soymilk in a water-bath for 15 min at 75 and 100 degrees C, TI activity decreased to 35 and 12%, respectively. The TI activity of tofu was proportional to the remaining whey. The effects of chemicals used for the coagulation of soy protein and foam-removal on TI activity were little. The results suggest that soybean products retain 2.5-12.5% TI activity of the whole soybean and that humans are consuming some active TI in their daily lives.
Neuroendocrine prediction of left ventricular function and heart failure after acute myocardial infarction (1999)

Plasma BNP measured within 1-4 days of acute myocardial infarction is a powerful independent predictor of left ventricular function, heart failure, or death over the subsequent 14 months, and superior to ANF, N-ANF, cGMP, and plasma catecholamines.
Recurrent Post-partum Jaundice: Rare Genetic Disorder With Novel Genetic Mutations Identified (2015)

Defective secretion of bile salts results in the accumulation of bile salts and toxic by-products in hepatocytes, as well as plasma, which leads to progressive liver fibrosis and/or cirrhosis. Mutations in the ABCB11 gene, encoding BSEP (bile salt export pump), are associated with benign recurrent intrahepatic cholestasis type 2 (BRIC2) and progressive familial intrahepatic cholestasis type 2 (PFIC2), both rare genetic diseases with an incidence of 1/50,000 to 1/100,000 births. We report two novel mutations in the ABCB11 gene resulting in severe bile salt export deficiency in a patient with recurrent episodes of jaundice and liver failure after multiple subsequent pregnancies. A 27-year-old G4P4 female developed worsening jaundice, pruritus, and abdominal pain 8 weeks after her fourth pregnancy. Laboratory results were significant for elevated total bilirubin of 17.1 mg/dL, direct bilirubin of 12.6 mg/dL, AST 76 IU/L, ALT 45 IU/L, and ALP 358 IU/L. GGT levels were within normal limits at 16 IU/L, INR 1.4, PT 14.2 and albumin 4.1 g/dL. The patient had a history of four successful pregnancies with uncomplicated deliveries. 5-8 weeks after each delivery she developed severe jaundice and liver dysfunction characterized by a similar pattern of laboratory abnormalities. Each episode of jaundice after pregnancy lasted progressively longer, ranging from 4 weeks to 10 months. Our patient underwent an extensive workup for infectious, autoimmune, obstructive, and drug-induced etiologies of disease including multiple liver biopsies, cholecystectomy, and bile duct stenting. After the third pregnancy the patient underwent genetic testing where a heterozygous frameshift mutation, c.2542delG, and a heterozygous missense variant, c.245T>C, were detected in the ABCB11 gene, which encodes for BSEP. We describe two novel mutations in the ABCB11 gene associated with the development of BRIC in a 27 yo female patient with recurrent postpartum jaundice. There have been no reported cases of BRIC2 presenting after pregnancy in a consistent manner as observed in our patient. One explanation could be linked to the estrogen-induced cholestasis in the presence of estradiol-17B-glucuronide (E217G), a cholestatic estrogen metabolite that could exacerbate the onset of postpartum jaundice and cholestasis. BRIC should be considered in the differential diagnosis of patients with recurrent jaundice of unknown etiology.
The lipoprotein-associated coagulation inhibitor that inhibits the factor VII-tissue factor complex also inhibits factor Xa: insight into its possible mechanism of action (1988)

Blood coagulation is initiated when plasma factor VII(a) binds to its essential cofactor tissue factor (TF) and proteolytically activates factors X and IX. Progressive inhibition of TF activity occurs upon its addition to plasma. This process is reversible and requires the presence of VII(a), catalytically active Xa, Ca2+, and another component that appears to be associated with the lipoproteins in plasma, a lipoprotein-associated coagulation inhibitor (LACI). A protein, LACI(HG2), possessing the same inhibitory properties as LACI, has recently been isolated from the conditioned media of cultured human liver cells (HepG2). Rabbit antisera raised against a synthetic peptide based on the N-terminal sequence of LACI(HG2) and purified IgG from a rabbit immunized with intact LACI(HG2) inhibit the LACI activity in human serum. In a reaction mixture containing VIIa, Xa, Ca2+, and purified LACI(HG2), the apparent half-life (t1/2) for TF activity was 20 seconds. The presence of heparin accelerated the initial rate of inhibition threefold. Antithrombin III alpha alone had no effect, but antithrombin III alpha with heparin abrogated the TF inhibition. LACI(HG2) also inhibited Xa with an apparent t1/2 of 50 seconds. Heparin enhanced the rate of Xa inhibition 2.5-fold, whereas phospholipids and Ca2+ slowed the reaction 2.5-fold. Xa inhibition was demonstrable with both chromogenic substrate (S-2222) and bioassays, but no complex between Xa and LACI(HG2) could be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Nondenaturing PAGE, however, showed that LACI(HG2) bound to Xa but not to X or Xa inactivated by diisopropyl fluorophosphate. Thus, LACI(HG2) appears to bind to Xa at or near its active site. Bovine factor Xa lacking its gamma-carboxyglutamic acid-containing domain, BXa(-GD), through treatment with alpha-chymotrypsin, was used to further investigate the Xa requirement for VIIa/TF inhibition by LACI(HG2). LACI(HG2) bound to BXa(-GD) and inhibited its catalytic activity against a small molecular substrate (Spectrozyme Xa), though at a rate approximately sevenfold slower than native BXa. Preincubation of LACI(HG2) with saturating concentrations of BXa(-GD) markedly retarded the subsequent inhibition of BXa. The VII(a)/TF complex was not inhibited by LACI(HG2) in the presence of BXa(-GD), and further, preincubation of LACI(HG2) with BXa(-GD) slowed the inhibition of VIIa/TF after the addition of native Xa. The results are consistent with the hypothesis that inhibition of VII(a)/TF involves the formation of a VIIa-TF-XA-LACI complex that requires the GD of XA.(ABSTRACT TRUNCATED AT 400 WORDS).
Recent epidemiology of Clostridium difficile infection during hematopoietic stem cell transplantation (2010)

Given the limited information on Clostridium difficile infection (CDI) during hematopoietic stem cell transplantation (HSCT), we examined the recent epidemiology of CDI in HSCT recipients at our institution. During the two-yr retrospective study period (2005-2006), 361 transplants were performed: 60% allogeneic and 40% autologous. Among all hospitalized patients in a non-outbreak setting, CDI rates in HSCT recipients were ninefold higher than those in general patients and 1.4-fold higher than those in patients with cancer (24.0 vs. 2.6 vs. 16.8/10,000 patient-days respectively). Sixty-two episodes of CDI occurred in 51 (14%) HSCT recipients: 39 (18%) allogeneic vs. 12 (8%) autologous (p = 0.01). Almost half of CDI episodes occurred within 30 d post-HSCT and 22% before HSCT. Clostridium difficile toxin assay was initially positive in 28% of the first, 31% of the second and 27% of the third stool samples tested. All but one patient responded to therapy with metronidazole or vancomycin. Severe CDI occurred in one patient and recurrent CDI in two patients. CDI is common during HSCT especially in allogeneic transplants during the peri-HSCT period. Prospective studies to better define the epidemiology and identify unique risk factors for CDI and more accurate tests to confirm the diagnosis in this population are needed.
3D tracking via body radio reflections (2014)

This paper introduces WiTrack, a system that tracks the 3D motion of a user from the radio signals reflected off her body. It works even if the person is occluded from the WiTrack device or in a different room. WiTrack does not require the user to carry any wireless device, yet its accuracy exceeds current RF localization systems, which require the user to hold a transceiver. Empirical measurements with a WiTrack prototype show that, on average, it localizes the center of a human body to within a median of 10 to 13 cm in the x and y dimensions, and 21 cm in the z dimension. It also provides coarse tracking of body parts, identifying the direction of a pointing hand with a median of 11.2°. WiTrack bridges a gap between RF-based localization systems which locate a user through walls and occlusions, and human-computer interaction systems like Kinect, which can track a user without instrumenting her body, but require the user to stay within the direct line of sight of the device.
HLA Homology within the C5 Domain Promotes Peptide Binding by HIV Type 1 gp120 (2008)

The mechanisms by which HIV-1 induces chronic pathogenic immune activation associated with disease progression remain unclear despite many years of AIDS research. One proposal suggests that sequence and structural mimicry between gp120 and HLA may endow HIV with the capacity to arouse alloreactive and autoimmune responses within the susceptible host, fueling disease progression in a manner similar to graft-versus-host disease (GVHD). Both gp120 and HLA share a common functional interaction with CD4 but also demonstrate peptide binding properties. Here we report the conserved nature of this feature across HIV-1 envelopes, the crucial role of the HLA homologous C5 region for peptide interactions, and the elimination of this property through specific antibody targeting. Given that the C5 domain mimics a HLA activation domain and the reported clinical benefits associated with nonneutralizing antibodies against this region, targeting the C5 domain may have use as a therapeutic vaccine to protect against disease progression.
End-Capping Strategies for Triggering End-to-End Depolymerization of Polyglyoxylates (2016)

Polymers that undergo end-to-end depolymerization in response to the cleavage of a stimuli-responsive end-cap are promising for diverse applications from drug delivery to responsive coatings and plastics. It is critical that the end-cap is designed to respond to an appropriate stimulus for the application. In the current work, end-caps for triggering the depolymerization of poly(ethyl glyoxylate) (PEtG) were explored. First, a phenylboronate, a disulfide, and an azobenzene were utilized to impart redox-responsive properties to PEtG. Then, methoxy-substituted trityl groups were used to provide sensitivity to mild acid. A multiresponsive platform was also introduced, allowing PEtG to respond to multiple stimuli, either simultaneously or independently. Incorporation of a cross-linkable trialkene end-cap enabled the preparation of networks that could subsequently be depolymerized. Finally, high molar mass PEtG could be depolymerized by mechanical stimulation independent of the end-cap. It is anticipated that the versatility in end-capping strategies and potential depolymerization stimuli will not only expand PEtG's utility for different applications but also be useful for other classes of end-to-end depolymerizable polymers.
Hypoxia in human gliomas: demonstration by PET with fluorine-18-fluoromisonidazole. (1992)

Positron emission tomography (PET) with the hypoxic-cell tracer [18F]fluoromisonidazole presents a possible means of noninvasively demonstrating tumor hypoxia. PET studies using this tracer were performed in three patients with malignant glioma, and in all patients the tumor was clearly seen at 5 min postinjection and initial tumor activity exceeded cortical activity. In one patient, there was no tumor retention of [18F] fluoromisonidazole and tumor activity fell while cortical activity increased, with the two tissues reaching equality at 40-50 min. The tumor-to-plasma ratio was 0.71 at 3 hr. The other two patients showed variable tumor retention of [18F]fluoromisonidazole, with tumor-to-plasma ratios of 1.10 and 1.49 at 2 and 3 hr. These results demonstrate the feasibility of using [18F]fluoromisonidazole PET to detect hypoxia in human gliomas in vivo. Clinical trials are needed to determine whether a relationship exists between [18F]fluoromisonidazole uptake and tumor radiation response.