gebhart/patrepeval huggingface dataset:
Evaluation of an Absorbable Collagen Membrane in Treating Class II Furcation Defects (1994)

Recent research has focused upon the utilization of an absorbable collagen membrane in guided tissue regeneration (GTR). Concern exists as to whether this type of membrane is beneficial in the treatment of periodontal defects. The purpose of this study was to evaluate the effect of a type I bovine collagen membrane on treatment of Class II furcation defects. Twelve systemically healthy patients (six male and six female, ages 32 to 68) were treated. Each had bilateral mandibular furcation defects with attachment loss > or = 6 mm. Prior to surgery all patients completed initial therapy including scaling and root planing. At the time of the surgery, teeth were randomly assigned to either a control (flap debridement alone) or test (flap debridement plus collagen membrane) group. Data were collected on the day of surgery, and 2, 4, and 6 months post-surgery and at the 12 month re-entry surgery. Clinical measurements included probing depth (PD), clinical attachment level (CAL), gingival recession (GR), stent to base of defect (SB), crestal bone to base of defect (CB), width of defect, and mobility. Statistical analysis was performed utilizing the paired t test. Both control and test groups demonstrated significant (P < 0.05) improvement at 12 months re-entry in PD, CAL, SB, and CB when compared to the presurgery status. While there is no significant difference in PD, CAL, GR, width of defect, and mobility between control and test groups, sites treated with the collagen membrane had significantly higher bone fill (SB and CB) at re-entry.(ABSTRACT TRUNCATED AT 250 WORDS)
Screening for Ovarian Cancer: U.S. Preventive Services Task Force Reaffirmation Recommendation Statement (2012)

The USPSTF recommends against screening for ovarian cancer in women (D recommendation).
The role of the TolC family in protein transport and multidrug efflux. (2001)

Gram-negative bacteria are enveloped by a system of two membranes, and they use specialized multicomponent, energy-driven pumps to transport molecules directly across this double-layered partition from the cell interior to the extra-cellular environment. One component of these pumps is embedded in the outer-membrane, and the paradigm for its structure and function is the TolC protein from Escherichia coli. A common component of a wide variety of efflux pumps, TolC and its homologues are involved in the export of chemically diverse molecules ranging from large protein toxins, such as alpha-hemolysin, to small toxic compounds, such as antibiotics. TolC family members thus play important roles in conferring pathogenic bacteria with both virulence and multidrug resistance. These pumps assemble reversibly in a transient process that brings together TolC or its homologue, an inner-membrane-associated periplasmic component, an integral inner-membrane translocase and the substrate itself. TolC can associate in this fashion with a variety of different partners to participate in the transport of diverse substrates. We review here the structure and function of TolC and the other components of the efflux/transport pump.
Isolation and Culture of Human Macrophages (2004)

Methods to isolate and culture human monocyte-derived macrophages and alveolar macrophages are described. Monocytes are obtained from buffy-coat preparations by Ficoll density gradient centrifugation, followed by adhesion-mediated purification on tissue culture or gelatin-coated plastic. The monocytes differentiate into macrophages in vitro by culturing in medium containing autologous human fibrin-depleted plasma. Alveolar macrophages can be purified from bronchoalveolar fluid samples by adhesion to tissue culture plastic. If resected lung tissue is available, alveolar macrophages can be obtained by mechanically disrupting the lung parenchyma, followed by adhesion-mediated purification.
N2′→P3′ Phosphoramidate Glycerol Nucleic Acid as a Potential Alternative Genetic System (2009)

Glycerol nucleic acid (GNA) is an interesting base-pairing system with an acyclic, three-carbon backbone. In the present study, GNA analogues with N2'-->P3' phosphoramidate linkages (npGNA) have been synthesized and their base-pairing properties examined. Thermal denaturation and circular dichroism studies show that npGNA can form stable duplexes with itself and with GNA. Furthermore, we show that npGNA can be assembled by template-directed ligation of 3'-imidazole-activated-2'-amino GNA dinucleotides. These results suggest that npGNA is a potential candidate for a self-replicating system based upon phosphoramidate linkages.
Unmixing-Based Soft Color Segmentation for Image Manipulation (2017)

We present a new method for decomposing an image into a set of soft color segments that are analogous to color layers with alpha channels that have been commonly utilized in modern image manipulation software. We show that the resulting decomposition serves as an effective intermediate image representation, which can be utilized for performing various, seemingly unrelated, image manipulation tasks. We identify a set of requirements that soft color segmentation methods have to fulfill, and present an in-depth theoretical analysis of prior work. We propose an energy formulation for producing compact layers of homogeneous colors and a color refinement procedure, as well as a method for automatically estimating a statistical color model from an image. This results in a novel framework for automatic and high-quality soft color segmentation that is efficient, parallelizable, and scalable. We show that our technique is superior in quality compared to previous methods through quantitative analysis as well as visually through an extensive set of examples. We demonstrate that our soft color segments can easily be exported to familiar image manipulation software packages and used to produce compelling results for numerous image manipulation applications without forcing the user to learn new tools and workflows.
High speed aerodynamics of upper surface blowing aircraft configurations (1992)

An experimental investigation of the high speed aerodynamics of Upper Surface Blowing (USB) aircraft configurations has been conducted to accurately define the magnitude and causes of the powered configuration cruise drag. A highly instrumented wind tunnel model of a realistic USB configuration was used which permitted parametric variations in the number and spanwise location of the nacelles and was powered with two turbofan engine simulators. The tests conducted in the Ames 14 Foot Transonic Wind Tunnel examined 10 different configurations at Mach numbers from 0.5 to 0.775, fan nozzle pressure ratios from 1.1 to 2.1 and angles of attack from -4 to 6 degrees. Measured force data is presented which indicates the cruise drag penalty associated with each configuration and surface pressure contour plots are used to illustrate the underlying flowfield physics. It was found that all of the tested configurations suffered from a severe drag penalty which increased with freestream Mach number, power setting and angle of attack and was associated with the presence of strong shocks and regions of separated flow in the wing/nacelle junction regions.
Assessing skin hydration status in haemodialysis patients using terahertz spectroscopy: a pilot/feasibility study (2008)

Terahertz (THz) time-domain spectroscopy was used in vivo to measure the body reflectance with the aim of determining experimentally the influence of haemodialysis on the content of water in the human skin. For this purpose, an original methodology of determining the skin properties at THz frequencies from the reflectance was developed. A series of measurements were performed before and after dialysis with ten subjects. The results strongly indicate that the surface body hydration is not the main parameter determining the skin conductivity in the THz range.
Built-In Soft Error Resilience for Robust System Design (2007)

Built-in soft error resilience (BISER) is an architecture-aware circuit design technique for correcting soft errors in latches, flip-flops and combinational logic. BISER enables more than an order of magnitude reduction in chip-level soft error rate with minimal area impact, 6-10% chip-level power impact, and 1-5% performance impact (depending on whether combinational logic error correction is implemented or not). In comparison, several traditional error-detection techniques introduce 40-100% power, performance and area penalties, and require significant efforts for designing and validating corresponding recovery mechanisms. In addition, BISER enables system design with configurable soft error protection features. Such features are extremely important for future designs targeting applications with a wide range of power, performance and reliability constraints. Design trade-offs associated with BISER and other existing soft error protection techniques are also analyzed.
Field of a parametric focusing source (1983)

An analytical description for the field of a parametric focusing source is derived. It is valid for spherically concave sources with small aperture angle and high ka, under conditions of quasilinear interaction (strong shocks precluded). The solution furnishes computations on the phase and amplitude of difference frequency sound along the axis and in the focal plane, as well as on the width of the radiation lobe in the focal region. Underwater experiments conducted with an f/2 lens coupled to a dual, interleaved primary array are discussed. The results support the utility and validity of the analytical model for describing the distribution of sound along the acoustic axis and across the focal plane. The difference frequency radiation was found to be effectively focused, in that the width of the beam became quite narrow in the focal plane.
Prostate cancer knowledge among multiethnic black men. (2004)

The purpose of this survey of 528 black men in Miami and Fort Lauderdale, FL, was to assess prostate cancer knowledge among African-American, English-speaking Caribbean, Haitian-American, and African men in America. Knowledge levels were assessed by education, ethnicity, age, income, family history of prostate cancer, and perceived prostate cancer knowledge. Twenty-five barbershops were visited during Fridays, Saturdays, and Sundays over a course of five months. The response rate was 99%. As the black men waited to be seen by their barbers, three interviewers asked them to answer the demographic and knowledge questions. SPSS was used to analyze the data. The main findings were that knowledge levels were adequate, with mean correct responses being 68.4%. Approximately 19.1% of respondents answered 80% of questions correctly, and 7.1% answered 100% of questions correctly. There were no statistically significant differences in knowledge among black men of different ethnicities. There were only two factors which were significantly related to prostate cancer knowledge. Men who earned more than dollars 50,000 and those with a family history of prostate cancer scored significantly higher than lower-income men and men with no family history of prostate cancer. As the percentage of Caribbean-American men and African men in the United States continues to increase, especially in metropolitan centers, those men at highest risk need to be targeted vigorously so that the disproportionate burden of prostate cancer on black men can be relieved.
Macrophages within NSCLC tumour islets are predominantly of a cytotoxic M1 phenotype associated with extended survival (2008)

There is a marked survival advantage for patients with nonsmall cell lung cancer (NSCLC) expressing high numbers of macrophages in their tumour islets. The primary aim of the present study was to determine the immunological phenotype of NSCLC-associated macrophages. CD68(+) macrophages expressing markers of a cytotoxic M1 phenotype or a noncytotoxic M2 phenotype were identified in the islets and stroma of surgically resected tumours from 20 patients with extended survival (median 92.7 months) and 20 with poor survival (median 7.7 months), using immunohistochemistry. The islet density of both M1 and M2 macrophages was markedly increased in extended compared with poor survival patients. In the extended survival group, M1 islet density was significantly increased compared with M2 density, 70% of islet macrophages were positive for M1 markers versus 38% for M2, and the islet:stromal ratio of M1 macrophages was markedly increased compared with M2. The 5-yr survival for patients with above and below median expression of M1 macrophages in the islets was >75 and <5%, respectively. Macrophages infiltrating the tumour islets in nonsmall cell lung cancer were predominantly of the M1 phenotype in patients with extended survival. The survival advantage conferred by islet macrophage infiltration may be related to their cytotoxic antitumour activity.
Construction, expression and characterization of a soluble form of human endothelin‐converting‐enzyme‐1 (1997)

Endothelin-converting-enzyme-1 (ECE-1) belongs to the family of zinc metallopeptidases and is responsible for generating endothelin (ET) peptides from their inactive precursors the big endothelins (bigET). The enzyme is a type II integral membrane protein consisting of a short amino-terminal cytosolic domain of 56 amino acids, a single transmembrane domain and a large putative extracellular domain containing the catalytic site. Recombinant and native ECE-1 are expressed as a dimer. We have constructed a soluble form of ECE, named sECE*, by fusing the cleavable signal peptide of pro-opiomelanocortin in frame to the complete extracellular domain of human ECE-1. Stable expression of this construct in CHO cells resulted in the secretion of a fully active enzyme. In contrast to membrane-bound ECE, sECE* was expressed as a monomer, highly glycosylated, as assessed by gel filtration and Western blot. However, recombinant sECE* converted bigET-1 with similar specific activity as ECE-1a. This activity was completely inhibited by phosphoramidon, but not by thiorphan and captopril. sECE* was active in a broad range of pH, showing an optimum of 6.6-6.8 for bigET-1. Thus, the extracellular domain alone is sufficient for conferring full ECE-1 activity, inhibitors recognition and substrate specificity.
Simultaneous routing and buffer insertion with restrictions on buffer locations (2000)

During the routing of global interconnects, macro blocks form useful routing regions which allow wires to go through but forbid buffers to be inserted. They give restrictions on buffer locations. In this paper, we take these buffer location restrictions into consideration and solve the simultaneous maze routing and buffer insertion problem. Given a block placement defining buffer location restrictions and a pair of pins (a source and a sink), we give a polynomial time exact algorithm to find a buffered route from the source to the sink with minimum Elmore delay.
Design of a low-latency asynchronous adder using speculative completion (1996)

A new general method for designing asynchronous datapath components, called speculative completion, is introduced. The method has many of the advantages of a bundled data approach, such as the use of single-rail synchronous datapaths, but it also allows early completion. As a case study, the method is applied to the high-performance parallel BLC adder design of Brent and Kung. Through careful gate-level analysis, performance improvements of up to 30% over a comparable synchronous implementation are expected.
Targeted inhibition of Hsp90 by ganetespib is effective across a broad spectrum of breast cancer subtypes (2013)

Heat shock protein 90 (Hsp90) is a molecular chaperone essential for the stability and function of multiple cellular client proteins, a number of which have been implicated in the pathogenesis of breast cancer. Here we undertook a comprehensive evaluation of the activity of ganetespib, a selective Hsp90 inhibitor, in this malignancy. With low nanomolar potency, ganetespib reduced cell viability in a panel of hormone receptor-positive, HER2-overexpressing, triple-negative and inflammatory breast cancer cell lines in vitro. Ganetespib treatment induced a rapid and sustained destabilization of multiple client proteins and oncogenic signaling pathways and even brief exposure was sufficient to induce and maintain suppression of HER2 levels in cells driven by this receptor. Indeed, HER2-overexpressing BT-474 cells were comparatively more sensitive to ganetespib than the dual HER2/EGFR tyrosine kinase inhibitor lapatinib in three-dimensional culture. Ganetespib exposure caused pleiotropic effects in the inflammatory breast cancer line SUM149, including receptor tyrosine kinases, MAPK, AKT and mTOR signaling, transcription factors and proteins involved in cell cycle, stress and apoptotic regulation, as well as providing combinatorial benefit with lapatinib in these cells. This multimodal activity translated to potent antitumor efficacy in vivo, suppressing tumor growth in MCF-7 and MDA-MB-231 xenografts and inducing tumor regression in the BT-474 model. Thus, ganetespib potently inhibits Hsp90 leading to the degradation of multiple clinically-validated oncogenic client proteins in breast cancer cells, encompassing the broad spectrum of molecularly-defined subtypes. This preclinical activity profile suggests that ganetespib may offer considerable promise as a new therapeutic candidate for patients with advanced breast cancers.
Boosting T Cell-Mediated Immunity to Tyrosinase by Vaccinia Virus-Transduced, CD34+-Derived Dendritic Cell Vaccination (2004)

These results indicate that vaccination with MVA-hTyr-transduced dendritic cells is well tolerated, can possibly produce clinical responses, and activates tyrosinase- and vaccinia virus-specific T cells in vivo. These data suggest a broad utility of the MVA vector for targeting tumor-associated antigens to dendritic cells for tumor immunotherapy.
Chemokines and the pathophysiology of neuropathic pain (2007)

Chemokines and chemokine receptors are widely expressed by cells of the immune and nervous systems. This review focuses on our current knowledge concerning the role of chemokines in the pathophysiology of chronic pain syndromes. Injury- or disease-induced changes in the expression of diverse chemokines and their receptors have been demonstrated in the neural and nonneural elements of pain pathways. Under these circumstances, chemokines have been shown to modulate the electrical activity of neurons by multiple regulatory pathways including increases in neurotransmitter release through Ca-dependent mechanisms and transactivation of transient receptor channels. Either of these mechanisms alone, or in combination, may contribute to sustained excitability of primary afferent and secondary neurons within spinal pain pathways. Another manner in which chemokines may influence sustained neuronal excitability may be their ability to function as excitatory neurotransmitters within the peripheral and central nervous system. As is the case for traditional neurotransmitters, injury-induced up-regulated chemokines are found within synaptic vesicles. Chemokines released after depolarization of the cell membrane can then act on other chemokine receptor-bearing neurons, glia, or immune cells. Because up-regulation of chemokines and their receptors may be one of the mechanisms that directly or indirectly contribute to the development and maintenance of chronic pain, these molecules may then represent novel targets for therapeutic intervention in chronic pain states.
Promiscuous targeting of bromodomains by bromosporine identifies BET proteins as master regulators of primary transcription response in leukemia (2016)

Bromodomains (BRDs) have emerged as compelling targets for cancer therapy. The development of selective and potent BET (bromo and extra-terminal) inhibitors and their significant activity in diverse tumor models have rapidly translated into clinical studies and have motivated drug development efforts targeting non-BET BRDs. However, the complex multidomain/subunit architecture of BRD protein complexes complicates predictions of the consequences of their pharmacological targeting. To address this issue, we developed a promiscuous BRD inhibitor [bromosporine (BSP)] that broadly targets BRDs (including BETs) with nanomolar affinity, creating a tool for the identification of cellular processes and diseases where BRDs have a regulatory function. As a proof of principle, we studied the effects of BSP on leukemic cell lines known to be sensitive to BET inhibition and found, as expected, strong antiproliferative activity. Comparison of the modulation of transcriptional profiles by BSP after a short exposure to the inhibitor resulted in a BET inhibitor signature but no significant additional changes in transcription that could account for inhibition of other BRDs. Thus, nonselective targeting of BRDs identified BETs, but not other BRDs, as master regulators of context-dependent primary transcription response.
Passive optical telephony networks and broadband evolution (2003)

A passive optical local access network for telephony applications is proposed which can evolve a future broadband ISDN (integrated services digital network). A laboratory demonstrator has been constructed which can deliver a 144-kb/s ISDN channel, or the equivalent to 128 customers from a single fiber exchange. Options for evolution to broadband ISDN are discussed with emphasis on choice of multiplexing technique. A novel TDM (time-division multiplexing) sampling technique for demultiplexing and selecting digital video channels at the customer end is described. A 323-channel capability has been demonstrated at 2.2 Gb/s over an experimental optical network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Comparison of the Quantitative Methods for the Analysis of the Platinum-Containing Anticancer Drug {cis-[Amminedichloro(2-methylpyridine)]- platinum(II)} (ZD0473) by HPLC Coupled to Either a Triple Quadrupole Mass Spectrometer or an Inductively Coupled Plasma Mass Spectrometer (2003)

The use of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICPMS) as means for the quantitative determination of ZD0473, a platinum anticancer drug, and its related biologically active "aqua" compounds in biofluid samples is described. The performance of the resulting HPLC-ICPMS method was compared with that of a conventional HPLC-triple quadrupole mass spectrometer-based (HPLC-MS/MS) system for properties such as limit of detection, linearity, and reproducibility using spiked samples. The methods were then applied to the determination of plasma ultrafitrate concentrations of ZD0473 in dog plasma samples obtained following intravenous and oral administration at 0.5 and 6 mg/kg, respectively. These experiments showed that both methods were capable of providing accurate and precise results but that the HPLC-ICPMS method had advantages of extended linear range and superior sensitivity, providing a limit of quantification of 0.1 ng/mL for ZD0473, as compared to 5 ng/mL using the current HPLC-MS/MS method. In addition, by using a single combined HPLC-ICPMS/MS/MS system, it was possible to determine the relative MS/MS response of the aqua compounds for the first time.
Feasibility study for the non-invasive blood pressure estimation based on ppg morphology: normotensive subject study (2017)

Current study can estimate only the relative variation of blood pressure but could not find the absolute pressure value. Moreover, proposed index has the limitation of diastolic pressure tracing. However, the result shows that the proposed PI is statistically significantly correlated with blood pressures, and it suggests that the proposed PI as a promising additional parameter for the cuff less blood pressure monitoring.