gebhart/patrepeval huggingface dataset:
Synthesis of 3β‐Hydroxy[21‐14C]‐5β‐pregn‐8(14)‐en‐20‐one from Chenodeoxycholic Acid (1986)

Abstract 3β‐Hydroxy[21‐ 14 C]5β‐pregn‐8(14)‐en‐20‐one ( 17 ) was prepared from chenodeoxycholic acid ( 1a ). The synthetic sequence involved: ( i ) degradation of the bile‐acid side chain to an etianic acid; ( ii ) formation of the 8(14)‐double bond; ( iii ) inversion of the configuration at C(3); ( iv ) construction of the acetyl side chain at C(17) with the required isotopic label at C(21). Structures of all described products were confirmed by chemical and spectroscopic (IR, 1 H‐NMR, 13 C‐NMR, MS) methods.
Factors Associated with Persistence and Resumption of Insulin Pen Use for Patients with Type 2 Diabetes (2010)

results suggest that physician recommendations of pen use, patient perceptions of pens, and cost and insurance coverage of pens may play an important role in persistence of pen use. Among former pen users, duration of pen use and perceptions of pens may be important factors in likelihood of patients' resuming pen use.
Complement Receptor 1 Gene Polymorphisms Are Associated with Idiopathic Pulmonary Fibrosis (2003)

Idiopathic pulmonary fibrosis (IPF) is a chronic, fibrotic disorder underlain by aberrant wound healing of repeated lung injury. Environmental triggers and genetic background are likely to act as modifiers of the fibrotic response. Erythrocyte complement receptor 1 is a membrane protein mediating the transport of immune complexes to phagocytes. Three gene polymorphisms are related to the erythrocyte surface density of complement receptor 1 molecules, which in turn are related to the rate of immune complexes' clearance. There is evidence of association between sarcoidosis and the complement receptor 1 gene. We wondered whether IPF is associated with the complement receptor 1 gene alleles coding for a reduced molecule/erythrocyte ratio. We studied 74 patients and 166 control subjects. Three polymorphic sites of the gene, A3650G exon 22, HindIII RFLP intron 27, and C5507G exon 33, were analyzed and found to be in linkage disequilibrium. The GG genotype for the C5507G exon 33 polymorphism was significantly more common in patients with IPF than in control subjects (odds ratio = 6.232, 95% confidence interval = 2.198-18.419, p = 0.00023). The significant difference was found in both sexes. These findings agree with speculations on the role of the complement receptor 1 gene in idiopathic pulmonary fibrosis.
α10: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells (2001)

We report the cloning and characterization of rat alpha10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the alpha10 nAChR subunit gene is most similar to the rat alpha9 nAChR, and both alpha9 and alpha10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with alpha10 cRNA alone or in pairwise combinations with either alpha2-alpha6 or beta2-beta4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of alpha9 and alpha10 cRNAs resulted in the appearance of an unusual nAChR subtype. Compared with homomeric alpha9 channels, the alpha9alpha10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca(2+) ions. The pharmacological profiles of homomeric alpha9 and heteromeric alpha9alpha10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells. Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both alpha9 and alpha10 subunits.
An unexpected chiral spiro tetramer offers mechanistic insight into an improved sodium cyanide initiated polymerization of n-hexyl isocyanate in toluene (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn unexpected chiral spiro tetramer offers mechanistic insight into an improved sodium cyanide initiated polymerization of n-hexyl isocyanate in tolueneYoshio Okamoto, Yoshikazu Nagamura, Koichi Hatada, Chetan Khatri, and Mark M. GreenCite this: Macromolecules 1992, 25, 20, 5536–5538Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September 1992https://pubs.acs.org/doi/10.1021/ma00046a066https://doi.org/10.1021/ma00046a066research-articleACS PublicationsRequest reuse permissionsArticle Views166Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Transcription Factor STAT3 in Leptin Target Neurons of the Rat Hypothalamus (1998)

Leptin is an adipose tissue-derived hormone that regulates body weight via interactions with hypothalamic neuronal circuitries expressing specific leptin receptors (Ob-R). The Ob-Rs act via the JAK-STAT (Janus kinase-signal transducers and activators of transcription) pathway of signal transduction. Recent evidence suggests that primarily the transcription factor STAT3 mediates leptin's action in the hypothalamus. We have investigated the presence and cellular localization of STAT3 protein in the rat hypothalamus by means of indirect immunofluorescence histochemistry using a rabbit polyclonal STAT3 antiserum. The antiserum identified a 92-kDa protein using Western blotting on rat hypothalamic homogenates, corresponding to the expected size of STAT3. STAT3 immunoreactivity was demonstrated in Ob-R-containing neurons of the paraventricular nucleus (parvocellular part), periventricular nucleus, arcuate nucleus and in the lateral hypothalamic area. Direct double-labeling showed presence of STAT3 immunoreactivity in neuropeptide Y (NPY)-containing neurons of the ventromedial part of the arcuate nucleus and in proopiomelanocortin (POMC)-containing neurons of the ventrolateral part of the arcuate nucleus. The results provide an anatomical basis for a leptin action mediated by STAT3 in Ob-R-containing NPY and POMC neurons of the arcuate nucleus, as well as by Ob-R-containing neurons of the parvocellular paraventricular nucleus and lateral hypothalamic area.
ABH and Related Histo‐Blood Group Antigens; Immunochemical Differences in Carrier Isotypes and Their Distribution1 (1989)

This review summarizes present knowledge of the chemistry of histo-blood group ABH and related antigens. Recent advances in analytical carbohydrate chemistry (particularly mass spectrometry and NMR spectroscopy) and the introduction of monoclonal antibodies (MoAbs) have made it possible to distinguish structural variants of histo-blood group ABH antigens. Polymorphism of ABH antigens is induced by: (i) variations in peripheral core structure, of which four (type 1, 2, 3 and 4) are known in man; (ii) variation in inner core by branching process (blood group iI), leading to variation of unbranched vs. branched ABH determinants; (iii) biosynthetic interaction with other glycosyltransferases (Lewis, P. T/Tn blood systems) capable of acting on the same substrate as the ABH-defined transferases, and finally (iv) the nature of the glycoconjugate (glycolipid, glycoprotein of N- or O-linked type). ABH variants induced by item (i) above have been clearly distinguished qualitatively by MoAbs; e.g., at least six types of A determinants can be distinguished by qualitatively different classes of antibody. The variants induced by item (ii) create mono- vs. bivalent antigens which may be responsible for observed differences in antibody-binding affinity. Detailed studies of the chemistry of these antigens have increased our insight into blood groups, providing the basis for blood group iI and A subgrouping, as well as a relation between the ABH and Lewis, P, and T/Tn systems. A survey of the literature on distribution patterns of ABH variants is presented. It has been assumed that expression of histo-blood group antigens is developmentally regulated. Relationships between histo-blood group expression, development, differentiation and maturation, as well as malignant transformation, are discussed.
Anti-tubulin drugs conjugated to anti-ErbB antibodies selectively radiosensitize (2016)

Tumour resistance to radiotherapy remains a barrier to improving cancer patient outcomes. To overcome radioresistance, certain drugs have been found to sensitize cells to ionizing radiation (IR). In theory, more potent radiosensitizing drugs should increase tumour kill and improve patient outcomes. In practice, clinical utility of potent radiosensitizing drugs is curtailed by off-target side effects. Here we report potent anti-tubulin drugs conjugated to anti-ErbB antibodies selectively radiosensitize to tumours based on surface receptor expression. While two classes of potent anti-tubulins, auristatins and maytansinoids, indiscriminately radiosensitize tumour cells, conjugating these potent anti-tubulins to anti-ErbB antibodies restrict their radiosensitizing capacity. Of translational significance, we report that a clinically used maytansinoid ADC, ado-trastuzumab emtansine (T-DM1), with IR prolongs tumour control in target expressing HER2+ tumours but not target negative tumours. In contrast to ErbB signal inhibition, our findings establish an alternative therapeutic paradigm for ErbB-based radiosensitization using antibodies to restrict radiosensitizer delivery.
A general critique of inertial-electrostatic confinement fusion systems (1995)

The suitability of various implementations of inertial-electrostatic confinement (IEC) systems for use as D–T, D–D, D–3He, 3He–3He, p–11B, and p–6Li reactors has been examined, and several fundamental flaws in the concept have been discovered. Bremsstrahlung losses for all of these fuels have been calculated in a general fashion which applies not only to IEC systems but also to most other fusion schemes; these calculations indicate that bremsstrahlung losses will be prohibitively large for 3He–3He, p–11B, and p–6Li reactors and will be a considerable fraction of the fusion power for D–3He and D–D reactors. Further calculations show that it does not appear possible for the dense central region of a reactor-grade IEC device to maintain significantly non-Maxwellian ion distributions or to keep two different ion species at significantly different temperatures, in contradiction with earlier claims made about such systems. Since the ions form a Maxwellian distribution with a mean energy not very much smaller than the electrostatic well depth, ions in the energetic tail of the distribution will be lost at rates greatly in excess of the fusion rate. Even by using one of the best electron confinement systems proposed for such devices, a polyhedral cusp magnetic field, and by making exceedingly optimistic assumptions about the performance of that confinement system, the electron losses from the machine prove to be intolerable for all fuels except perhaps DT. In order for IEC systems to be used as fusion reactors, it will be necessary to find methods to circumvent these problems.
Fully competent dendritic cells as inducers of T cell anergy in autoimmunity (2000)

Mature immunologically competent dendritic cells are the most efficient antigen-presenting cells that powerfully activate T cells and initiate and sustain immune responses. Indeed, dendritic cells are able to efficiently capture antigens, express high levels of costimulatory molecules, and produce the combination of cytokines required to create a powerful immune response. They are also considered to be important in initiating autoimmune disease by efficiently presenting autoantigens to self-reactive T cells that, in this case, will mount a pathogenic autoimmune reaction. Triggering T cells is not a simple on-off procedure, as T cell receptor responds to minor changes in ligand with gradations of T cell activation and effector functions. These "misfit" peptides have been called Altered Peptide Ligands, and have been shown to have important biological significance. Here, we show that fully capable dendritic cells may present, upon natural antigen processing, a self-epitope with Altered Peptide Ligands features that can unexpectedly induce anergy in a human autoreactive T cell clone. These results indicate that presentation of a self-epitope by immunologically competent dendritic cells does not always mean "danger" and show a mechanism involved in the fine balance between activation and tolerance induction in humans.
Chicken embryonic stem cells as a non‐mammalian embryonic stem cell model (2010)

Embryonic stem cells (ESCs) were isolated in the early 1980s from mouse and in the late 1990s from primate and human. These cells present the unique property of self-renewal and the ability to generate differentiated progeny in all embryonic lineages both in vitro and in vivo. The mESCs (mouse embryonic stem cells) can contribute to both somatic and germinal lineages once re-injected into a recipient embryo at the blastocyst stage. In avian species, chicken embryonic stem cells (cESCs) have been isolated from the in vitro culture of early chicken blastodermal cells (cBCs) taken from stage X embryo (EG&K) These cESCs can be maintained under specific culture conditions and have been characterized on the basis of their morphology, biochemical features, in vitro differentiation potentialities and in vivo morphogenetic properties. The relationship between these cESCs and some of the chicken germ cells identified and grown under specific culture conditions are still under debate, in particular with the identification of the Cvh gene as a key factor for germ cell determination. Moreover, by cloning the avian homologue of the Oct4 mammalian gene, we have demonstrated that this gene, as well as the chicken Nanog gene, was involved in the characterization and maintenance of the chicken pluripotency. These first steps toward the understanding of pluripotency control in a non-mammalian species opens the way for the development and characterization of putative new cell types such as chicken EpiSC and raises the question of the existence of reprogramming in avian species. These different points are discussed.
Lookup-Table-Based Gradient Field Reconstruction (2011)

In computer vision, there are many applications, where it is advantageous to process an image in the gradient domain and then reintegrate the gradient field: important examples include shadow removal, lightness calculation, and data fusion. A serious problem with this approach is that the reconstruction step often introduces artefacts-commonly, smoothed and smeared edges-to the recovered image. This is a result of the inherent ill-posedness of reintegrating a nonintegrable field. Artefacts can be diminished but not removed, by using complex to highly complex reintegration techniques. Here, we present a remarkably simple (and on the face of it naive) algorithm for reconstructing gradient fields. Suppose we start with a multichannel original, and from it derive a (possibly one of many) 1-D gradient field; for many applications, the derived gradient field will be nonintegrable. Here, we propose a lookup-table-based map relating the multichannel original to a reconstructed scalar output image, whose gradient best matches the target gradient field. The idea, at base, is that if we learn how to map the gradients of the multichannel original onto the desired output gradient, and then using the lookup table (LUT) constraint, we effectively derive the mapping from the multichannel input to the desired, reintegrated, image output. While this map could take a variety of forms, here we derive the best map from the multichannel gradient as a (nonlinear) function of the input to each of the target scalar gradients. In this framework, reconstruction is a simple equation-solving exercise of low dimensionality. One obvious application of our method is to the image-fusion problem, e.g., the problem of converting a color or higher-D image into grayscale. We will show, through extensive experiments and complementary theoretical arguments, that our straightforward method preserves the target contrast as well as do complex previous reintegration methods, but without artefacts, and with a substantially cheaper computational cost. Finally, we demonstrate the generality of the method by applying it to gradient field reconstruction in an additional area, the shading recovery problem.
Anatomy of the pig heart: comparisons with normal human cardiac structure (1998)

Transgenic technology has potentially solved many of the immunological difficulties of using pig organs to support life in the human recipient. Nevertheless, other problems still remain. Knowledge of cardiac anatomy of the pig (Sus scrofa) is limited despite the general acceptance in the literature that it is similar to that of man. A qualitative analysis of porcine and human cardiac anatomy was achieved by gross examination and dissection of hearts with macrophotography. The porcine organ had a classic 'Valentine heart' shape, reflecting its location within the thorax and to the orientation of the pig's body (unguligrade stance). The human heart, in contrast, was trapezoidal in silhouette, reflecting man's orthograde posture. The morphologically right atrium of the pig was characterised by the tubular shape of its appendage (a feature observed on the left in the human heart). The porcine superior and inferior caval veins opened into the atrium at right angles to one another, whereas in man the orifices were directly in line. A prominent left azygous vein (comparable to the much reduced left superior caval or oblique vein in man) entered on the left side of the pig heart and drained via the coronary sinus. The porcine left atrium received only 2 pulmonary veins, whereas 4 orifices were generally observed in man. The sweep between the inlet and outlet components of the porcine right ventricle was less marked than in man, and a prominent muscular moderator band was situated in a much higher position within the porcine right ventricle compared with that of man. The apical components of both porcine ventricles possessed very coarse trabeculations, much broader than those observed in the human ventricles. In general, aortic-mitral fibrous continuity was reduced in the outlet component of the porcine left ventricle, with approximately two-thirds of the aortic valve being supported by left ventricular musculature. Several potentially significant differences exist between porcine and human hearts. It is important that these differences are considered as the arguments continue concerning the use of transgenic pig hearts for xenotransplantation.
Iodixanol, Constriction of Medullary Descending Vasa Recta, and Risk for Contrast Medium–induced Nephropathy (2009)

Iodixanol in doses typically used for coronary interventions constricts DVR, intensifies angiotensin II-induced constriction, and reduces bioavailability of NO. CM-induced nephropathy may be related to these events and scavenging of reactive oxygen species might exert a therapeutic benefit by preventing the adverse effects that a CM has on medullary perfusion.
Pure versus follicular variant of papillary thyroid carcinoma (2003)

The pathologic and clinical behaviors of PTC and FVPTC were comparable. Prognostic factors, treatment, and survival also were similar. Patients in both groups must be treated identically.
Adaptive compression coding (1991)

A compression technique which preserves edges in compressed pictures is developed. The proposed compression algorithm adapts itself to the local nature of the image. Smooth regions are represented by their averages and edges are preserved using quad trees. Textured regions are encoded using BTC (block truncation coding) and a modification of BTC using look-up tables. A threshold using a range which is the difference between the maximum and the minimum grey levels in a 4*4 pixel quadrant is used. At the recommended value of the threshold (equal to 18), the quality of the compressed texture regions is very high, the same as that of AMBTC (absolute moment block truncation coding), but the edge preservation quality is far superior to that of AMBTC. Compression levels below 0.5-0.8 b/pixel may be achieved.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Enhancement of DNA Vaccine Potency through Coadministration of CIITA DNA with DNA Vaccines via Gene Gun (2008)

Administration of DNA vaccines via gene gun has emerged as an important form of Ag-specific immunotherapy. The MHC CIITA is a master regulator of MHC class II expression and also induces expression of class I molecules. We reasoned that the gene gun administration of CIITA DNA with DNA vaccines employing different strategies to improve MHC I and II processing could enhance DNA vaccine potency. We observed that DC-1 cells transfected with CIITA DNA lead to higher expression of MHC I and II molecules, leading to enhanced Ag presentation through the MHC I/II pathways. Furthermore, our data suggested that coadministration of DNA-encoding calreticulin (CRT) linked to human papillomavirus (HPV) 16 E6 Ag (CRT/E6) with CIITA DNA leads to enhanced E6-specific CD8(+) T cell immune responses in vaccinated mice. In addition, coadministration of the combination of CRT/E6 DNA with CIITA DNA and DNA encoding the invariant chain (Ii) linked to the pan HLA-DR-reactive epitope (Ii-PADRE) further enhanced E6-specific CD8(+) T cell immune responses in vaccinated mice. Treatment with the combination vaccine was also shown to enhance the antitumor effects and to prolong survival in TC-1 tumor-bearing mice. Vaccination with the combination vaccine also led to enhanced E6-specific CD8(+) memory T cells and to long-term protection against TC-1 tumors and prolonged survival in vaccinated mice. Thus, our findings suggest that the combination of CIITA DNA with CRT/E6 and Ii-PADRE DNA vaccines represents a potentially effective means to combat tumors in the clinical setting.