gebhart/patrepeval huggingface dataset:
Coronary flow: a new asset for the echo lab? (2004)

Non-invasive imaging of coronary blood flow by transthoracic Doppler echocardiography is an emerging diagnostic tool to study the left anterior descending (LAD)1–11 and posterior descending (PD) coronary arteries.12–15 With this new clinical application of echocardiography, we can directly measure changes in coronary flow velocity reserve (CFVR) at the very beginning of the ischaemic cascade, instead of looking at the consequences of ischaemia on myocardial contraction, as it is routinely done with dobutamine stress echocardiography and other stress tests.

Since its introduction in 1997,1,2 it has been clear that transthoracic coronary Doppler ultrasound could provide useful information in the diagnosis of coronary artery disease (CAD)3–15 follow-up of percutaneous coronary interventions,16–21 coronary recanalization in acute myocardial infarction (AMI),22–26 and coronary microcirculation.27–33.

The importance of measuring CFVR in routine clinical practice has been anticipated over 20 years ago by the physiologist Carl Honig: One of the principal tasks of a physician is to estimate the patient's reserves… Prognosis is an estimate of the rate at which this reserve may disappear, and therapy is designed to increase this reserve and to prevent or eliminate stresses that might compromise it.34 With this teaching in mind we have planned our seven-year work on transthoracic coronary Doppler ultrasound. In this review we will focus on the main clinical applications of transthoracic coronary Doppler ultrasound, and discuss the advantages, limitations and technical pitfalls of the method.

Some basic yet simple concepts should be assimilated before beginning this new technique, in order to reduce errors and misinterpretations.

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Coronary blood flow velocity should be measured from an apical window by pulsed Doppler ultrasound under colour-coding guide. The best long axis view in colour flow imaging should be obtained to maintain a <30° angle between flow and the Doppler beam. Correction for …
14-3-3ζ Down-regulates p53 in Mammary Epithelial Cells and Confers Luminal Filling (2008)

Recent progress in diagnostic tools allows many breast cancers to be detected at an early preinvasive stage. Thus, a better understanding of the molecular basis of early breast cancer progression is essential. Previously, we discovered that 14-3-3 zeta is overexpressed in >40% of advanced breast cancers, and this overexpression predicts poor patient survival. Here, we examined at what stage of breast disease 14-3-3 zeta overexpression occurs, and we found that increased expression of 14-3-3 zeta begins at atypical ductal hyperplasia, an early stage of breast disease. To determine whether 14-3-3 zeta overexpression is a decisive early event in breast cancer, we overexpressed 14-3-3 zeta in MCF10A cells and examined its effect in a three-dimensional culture model. We discovered that 14-3-3 zeta overexpression severely disrupted the acini architecture resulting in luminal filling. Proper lumen formation is a result of anoikis, apoptosis due to detachment from the basement membrane. We found that 14-3-3 zeta overexpression conferred resistance to anoikis. Additionally, 14-3-3 zeta overexpression in MCF10A cells and in mammary epithelial cells (MEC) from 14-3-3 zeta transgenic mice reduced expression of p53, which is known to mediate anoikis. Mechanistically, 14-3-3 zeta induced hyperactivation of the phosphoinositide 3-kinase/Akt pathway which led to phosphorylation and translocation of the MDM2 E3 ligase resulting in increased p53 degradation. Ectopic expression of p53 restored luminal apoptosis in 14-3-3 zeta-overexpressing MCF10A acini in three-dimensional cultures. These data suggest that 14-3-3 zeta overexpression is a critical event in early breast disease, and down-regulation of p53 is one of the mechanisms by which 14-3-3 zeta alters MEC acini structure and increases the risk of breast cancer.
Parallel Processor Array For High Speed Path Planning (1992)

The first integration of a 24 x 25 array of processors for high speed optimal path planning is reported. Based on programmed terrain costs (traversal time), the IC determines, in parallel, the fastest routes from a selected starting point(s) to all other points on a given tcrrain. The chip has hQen successfully tested at a 7 MHz clock frequency, with typical path determination requiring 230 lis, resulting in a four order of magnitude speed-up over currmt sofhvare-hasQd shortmtroute techniques.
Synthesis of ketobemidone precursors via phase‐transfer catalysis (1986)

Abstract Hydrochloride salts of N ‐substituted‐bis‐(2‐chloroethyl)amines can be condensed with m ‐methoxyphenyl‐acetonitrile or 1‐phenyl‐2‐alkanones under phase‐transfer conditions to yield precursors of the powerful analgesic compounds ‐ the ketobemidones.
The IFI‐56K and IFI‐54K interferon‐inducible human genes belong to the same gene family (1988)

The IFI-56K and IFI-54K human genes are coordinately regulated by interferon, double-stranded RNA and viruses in a number of cell lines. These genes encode polypeptides of 56 and 54 kDa, respectively, whose function remains to be determined. We analysed the possible structural relatedness between these syntenic and similarly regulated genes. We found that they are very closely related at the protein, mRNA and promoter levels. This suggests that the IFI-56K and IFI-54K genes are members of a gene family, which probably arose from duplication of an ancestor gene.
EXERCISE AND SKELETAL MUSCLE GLUCOSE TRANSPORTER 4 EXPRESSION: MOLECULAR MECHANISMS (2006)

1. Skeletal muscle is a highly plastic tissue that has a remarkable ability to adapt to external demands, such as exercise. Many of these adaptations can be explained by changes in skeletal muscle gene expression. A single bout of exercise is sufficient to induce the expression of some metabolic genes. We have focused our attention on the regulation of glucose transporter isoform 4 (GLUT-4) expression in human skeletal muscle. 2. Glucose transporter isoform 4 gene expression is increased immediately following a single bout of exercise, and the GLUT-4 enhancer factor (GEF) and myocyte enhancer factor 2 (MEF2) transcription factors are required for this response. Glucose transporter isoform enhancer factor and MEF2 DNA binding activities are increased following exercise, and the molecular mechanisms regulating MEF2 in exercising human skeletal muscle have also been examined. 3. These studies find possible roles for histone deacetylase 5 (HDAC5), adenosine monophosphate-activated protein kinase (AMPK), peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) and p38 mitogen-activated protein kinase (MAPK) in regulating MEF2 through a series of complex interactions potentially involving MEF2 repression, coactivation and phosphorylation. 4. Given that MEF2 is a transcription factor required for many exercise responsive genes, it is possible that these mechanisms are responsible for regulating the expression of a variety of metabolic genes during exercise. These mechanisms could also provide targets for the treatment and management of metabolic disease states, such as obesity and type 2 diabetes, which are characterized by mitochondrial dysfunction and insulin resistance in skeletal muscle.
Molecular Cloning of a 32-Kilodalton Lipoprotein Component of a Novel Iron-Regulated Staphylococcus epidermidis ABC Transporter (1998)

Our previous studies identified two iron-regulated cytoplasmic membrane proteins of 32 and 36 kDa expressed by both Staphylococcus epidermidis and Staphylococcus aureus. In this study we show by Triton X-114 phase partitioning and tritiated palmitic acid labelling that these proteins are lipoproteins which are anchored into the cytoplasmic membrane by their lipid-modified N termini. In common with those of some other gram-positive bacteria, these highly immunogenic lipoproteins were released from the bacterial cell into the culture supernatants, with release being promoted by growth of the bacteria under iron-restricted conditions. Immunoelectron microscopy with a monospecific rabbit antiserum to the 32-kDa S. epidermidis lipoprotein showed that the majority of the antigen was distributed throughout the staphylococcal cell wall. Only minor quantities were detected in the cytoplasmic membrane, and exposure of the lipoprotein on the bacterial surface was minimal. A monoclonal antibody raised to the 32-kDa lipoprotein of S. aureus was used in immunoblotting studies to investigate the conservation of this antigen among a variety of staphylococci. The monoclonal antibody reacted with polypeptides of 32 kDa in S. epidermidis and S. aureus and of 40 kDa in Staphylococcus hominis. No reactivity was detected with Staphylococcus lugdunensis, Staphylococcus cohni, or Staphylococcus haemolyticus. The gene encoding the 32-kDa lipoprotein from S. epidermidis has been isolated from a Lambda Zap II genomic DNA library and found to be a component of an iron-regulated operon encoding a novel ABC-type transporter. The operon contains three genes, designated sitA, -B, and -C, encoding an ATPase, a cytoplasmic membrane protein, and the 32-kDa lipoprotein, respectively. SitC shows significant homology both with a number of bacterial adhesins, including FimA of Streptococcus parasanguis and ScaA of Streptococcus gordonii, and with lipoproteins of a recently described family of ABC transporters with proven or putative metal ion transport functions. Although the solute specificity of this novel transporter has not yet been determined, we speculate that it may be involved in either siderophore- or transferrin-mediated iron uptake in S. epidermidis.
Cerium oxidation state in LSO:Ce scintillators (2005)

Trivalent cerium ions form the luminescence centers in several important families of scintillation materials including the rare earth oxyorthosilicates, pyrosilicates, and aluminates. When comparing the experimentally determined scintillation properties of cerium-doped scintillators to theoretical models of scintillation mechanisms, there is often speculation regarding the fraction of the total cerium that exists in the radiative trivalent charge state (Ce/sup 3+/) rather than the nonradiative tetravalent state (Ce/sup 4+/). Until now, however, no technique has been developed to quantitatively measure both Ce/sup 3+/ and Ce/sup 4+/ in single crystal scintillators . We report here for the first time direct measurements of Ce/sup 3+/ and Ce/sup 4+/ in Lu/sub 2/SiO/sub 5/:Ce single crystals. Synchrotron radiation was used to measure the X-ray absorption on the M/sub 4/ and M/sub 5/ edges of Ce, and the results were compared to model samples of Ce/sup 3+/ (Ce/sub 2/O/sub 3/) and Ce/sup 4+/ (CeO/sub 2/) which provided clear signatures of the two charge states. The spectra were obtained with a high-resolution superconducting tunnel junction spectrometer on beamline 4.0.2 at the Advanced Light Source synchrotron at Lawrence Berkeley National Laboratory. The results clearly show 100% Ce/sup 3+/, independent of light yield and sample coloration. Therefore, energy migration to the luminescence centers appears to be the determining factor in the scintillation efficiency of these samples, rather than variations in the Ce/sup 3+//Ce/sup 4+/ ratio.
Prolongation of the QT interval and cardiac arrhythmias associated with cisapride: limitations of the pharmacoepidemiological studies conducted and proposals for the future (2002)

Not all hazards can be identified from clinical studies prior to marketing of medicinal products. Pre-marketing large-scale trials for cisapride did not report any serious cardiac arrhythmias. After a long period of availability in several countries it was withdrawn in 2000 because of reports of serious, and in many cases fatal, cardiac events. Whilst spontaneous reporting systems for adverse drug reactions (ADRs) have limitations such as under-reporting, they are an effective system for signal generation, particularly of rare ADRs. Pharmacoepidemiological studies aim to identify and calculate the incidence of adverse reactions, with increased sensitivity to less common ADRs compared to randomised controlled trials, yet cohort sizes may be insufficient to detect very rare ADRs such as drug-induced Torsade de Pointes, with an estimated incidence of the order of 1 per 12,000 to 1 per 120,000 patients. Several pharmacoepidemiological studies investigated adverse events associated with cisapride, one of which specifically examined the association between serious cardiac arrhythmias and cisapride. These observational studies were conducted using large population databases, but each failed to identify sufficient cases to establish a causal relationship. Explanations include that the cohort sample sizes were too small, and either under-, or mis-reporting of events of interest may have occurred. To estimate the risk of very rare adverse events, pharmacoepidemiological studies require very large numbers. Furthermore, the events in question need to be clinically recognisable by doctors and adequately documented in patients' notes, computer records, or on study questionnaires. The establishment of a national registry for drug-induced QT prolongation to identify cases and correlate clinical information may help to better identify these rare ADRs earlier. Such proactive surveillance could avoid unnecessary delays for other drugs where QT prolongation and serious cardiac arrhythmias may be an issue.
Azidonucleosides:  Synthesis, Reactions, and Biological Properties (2002)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAzidonucleosides: Synthesis, Reactions, and Biological PropertiesTanmaya PathakView Author Information Department of Chemistry, Indian Institute of Technology, Kharagpur 721 302, India Cite this: Chem. Rev. 2002, 102, 5, 1623–1668Publication Date (Web):May 8, 2002Publication History Received14 September 2001Published online8 May 2002Published inissue 1 May 2002https://pubs.acs.org/doi/10.1021/cr0104532https://doi.org/10.1021/cr0104532research-articleACS PublicationsCopyright © 2002 American Chemical SocietyRequest reuse permissionsArticle Views2845Altmetric-Citations140LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Azides,Mixtures,Nucleic acids,Reaction products,Redox reactions Get e-Alerts
Tracking a few extreme singular values and vectors in signal processing (1990)

In various applications it is necessary to keep track of a low-rank approximation of a covariance matrix, R(t), slowly varying with time. It is convenient to track the left singular vectors associated with the largest singular values of the triangular factor, L(t), of its Cholesky factorization. These algorithms are referred to as square-root. The drawback of the eigenvalue decomposition (EVD) or the singular value decompositions (SVD) is usually the volume of the computations. Various numerical methods for carrying out this task are surveyed, and it is shown why this heavy computational burden is questionable in numerous situations and should be revised. Indeed, the complexity per eigenpair is generally a quadratic function of the problem size, but there exist faster algorithms with linear complexity. Finally, in order to make a choice among the large and fuzzy set of available techniques, comparisons based on computer simulations in a relevant signal processing context are made.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Effects of synthetic and natural lysophosphatidic acids on the arterial blood pressure of different animal species (1978)

Intravenous injection of lysophosphatidic acid was found to cause hypertension in rats and guinea pigs, but hypotension in cats and rabbits. The potencies of the pressor and depressor effects of synthetic lysophosphatidic acids in rats and cats depended on their chain length and the degree of unsaturation of their fatty acyl moieties.
Quantitative analysis of EO9 (apaziquone) and its metabolite EO5a in human plasma by high‐performance liquid chromatography under basic conditions coupled to electrospray tandem mass spectrometry (2006)

A sensitive and specific LC-MS/MS assay for the quantitative determination of EO9 and its metabolite EO5a is presented. A 200-microl human plasma aliquot was spiked with a mixture of deuterated internal standards EO9-d3 and EO5a-d4 and extracted with 1.25 ml ethyl acetate. Dried extracts were reconstituted in 0.1 M ammonium acetate-methanol (7 : 3, v/v) and 25 microl-volumes were injected into the HPLC system. Separation was achieved on a 150 x 2.1 mm C18 column using an alkaline eluent (1 mM ammonium hydroxide-methanol (gradient system)). Detection was performed by positive ion electrospray followed by tandem mass spectrometry. The assay quantifies a range from 5 to 2500 ng/ml for EO9 and from 10 to 2500 ng/ml EO5a using 200 microl of human plasma samples. Validation results demonstrate that EO9 and EO5a concentrations can be accurately and precisely quantified in human plasma. This assay will be used to support clinical pharmacologic studies with EO9.
Test–retest reliability of a group-administered paper–pencil measure of delay discounting. (2009)

The purpose of this study was to determine the reliability of an easily administered paper-pencil temporal discounting task modeled loosely on the hypothetical money choice task (Rachlin, Raineri, & Cross, 1991). Our group-administered task used 2 descriptions of discounting, the slope constant (k) of the hyperbolic function V = A/(1 + kD) and the area under the curve (AUC). The task was initially administered to 387 (163 men, 188 women, and 35 unspecified gender) introductory psychology students in groups ranging in size from 15 to 45. Six weeks later, 299 students (131 men and 168 women) repeated the task in groups ranging from 15 to 30. At each test time, the data from participants who met specified discounting criteria (about 85%) were fit well with the hyperbolic function (mean r(2) = .90). The test-retest correlation for logk was r = .64; for AUC scores, it was r = .70, with a correlation of r = -.96 between logk and AUC scores at Test 1. The (dislike for) Cognitive Complexity and Cognitive Instability subscales of the Barratt Impulsivity Scales, Version 11, administered only at the retest correlated with k values (r = .20, p = .001, and r = .179, p = .003), and women discounted more than men (p < .001) at both test times. The results are discussed in terms of the utility of the procedure and a detailed comparison of k and AUC measures of discounting.
Clinical Effects of the Recombinant Feline Interferon-.OMEGA. on Experimental Parvovirus Infection in Beagle Dogs. (1998)

The clinical effects of recombinant feline interferon-omega (rFeIFN-omega), produced in silkworm by recombinant baculovirus, were examined in 3-4 month-old beagle dogs given an experimental canine parvovirus type-2 (CPV-2) infection. Clinical symptoms, such as pyrexia, vomiting, anorexia and diarrhea, were observed on day 4 after oral inoculation of 10(7) TCID50 of CPV-2 (cc 238 strain) in almost all the inoculated dogs. From day 4, rFeIFN-omega (1 mega units/kg/day) or physiological saline was administered intravenously to infected dogs for 3 consecutive days. Seven out of 17 dogs treated with physiological saline showed hemorrhagic diarrhea and continuously expressed severe clinical enteritis; one dog died with a large amount of hemorrhagic rice-water stool on day 6 after viral exposure. In contrast, 4 out of 12 dogs treated with rFeIFN-omega showed severe clinical enteritis associated with intermittent diarrhea. Scoring of fecal condition revealed that treatment with rFeIFN-omega significantly shifted the enteritis from a severe to mild form. Furthermore, rFeIFN-omega administered in the morning decreased the number of dogs expressing clinical enteritis in the evening suggesting a rapid effect. Vomiting and anorexia were also improved by treatment with rFeIFN-omega. These results suggest that rFeIFN-omega can reduce severe enteritis caused by CPV-2 infection in dogs.
Efficient CVD Growth of Single-Walled Carbon Nanotubes on Surfaces Using Carbon Monoxide Precursor (2002)

Single Walled carbon nanotubes (SWNTs) were grown directly on flat substrates using chemical vapor deposition (CVD) method with carbon monoxide and hydrogen mixture as feeding gas. Comparing with other CVD methods, this new approach yields higher efficiency and more reproducible results in obtaining high quality SWNTs separated as individual nanotubes on substrates. Such samples can be used to fabricate nanodevices directly with no further purification or dispersion. Important factors that affect the nanotube growth and possible mechanisms are also discussed.
Effects of Yucca shidigera Extract and Soluble Protein on Performance of Cows and Concentrations of Urea Nitrogen in Plasma and Milk (1998)

Twelve multiparous Holstein cows averaging 122 d postpartum were used in a replicated Latin square design with 21-d periods. Cows were fed diets containing either low or high soluble protein supplemented with 0 or 9 g/d of Yucca shidigera extract per cow. Dry matter intake and yields of milk, fat, 3.5% fat-corrected milk, and total solids were not affected by treatment. High soluble protein increased percentages of fat and total solids in milk but decreased percentages of crude protein and true protein in milk. Yucca shidigera extract did not affect percentages or yields of milk components, ruminal NH3 N, or urea N in milk or plasma. High soluble protein tended to increase both ruminal pH and concentrations of ruminal NH3 N. Yucca shidigera extract did not affect ruminal pH or NH3 N. High soluble protein increased concentrations of urea N in plasma and milk when both were measured by enzymatic assay, but differences in milk urea N measured by mid infrared reflectance spectroscopy were not significant. Neither Yucca shidigera extract nor increased ruminally undegradable protein was beneficial in this trial when fed to cows milking 30 to 35 kg/d. Concentrations of urea N in milk as measured by mid infrared reflectance spectroscopy were higher than those measured by enzymatic assay, but measurements by mid infrared reflectance spectroscopy were not as sensitive to treatment differences as were measurements by enzymatic assay.