gebhart/patrepeval huggingface dataset:
A Deep Hierarchical Approach to Lifelong Learning in Minecraft (2016)

We propose a lifelong learning system that has the ability to reuse and transfer knowledge from one task to another while efficiently retaining the previously learned knowledge-base. Knowledge is transferred by learning reusable skills to solve tasks in Minecraft, a popular video game which is an unsolved and high-dimensional lifelong learning problem. These reusable skills, which we refer to as Deep Skill Networks, are then incorporated into our novel Hierarchical Deep Reinforcement Learning Network (H-DRLN) architecture using two techniques: (1) a deep skill array and (2) skill distillation, our novel variation of policy distillation (Rusu et. al. 2015) for learning skills. Skill distillation enables the HDRLN to efficiently retain knowledge and therefore scale in lifelong learning, by accumulating knowledge and encapsulating multiple reusable skills into a single distilled network. The H-DRLN exhibits superior performance and lower learning sample complexity compared to the regular Deep Q Network (Mnih et. al. 2015) in sub-domains of Minecraft.
IL2 Variant Circumvents ICOS+ Regulatory T-cell Expansion and Promotes NK Cell Activation (2016)

Clinical responses to high-dose IL2 therapy are limited due to selective expansion of CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> T-regulatory cells (Treg), especially ICOS<sup>+</sup> Tregs, rather than natural killer (NK) cells and effector T cells. These ICOS<sup>+</sup> Tregs are highly suppressive and constitutively express high levels of IL2Rα (CD25) and CD39. Here, we characterized the effect of a mutant form of IL2 (F42K), which preferentially binds to the lower affinity IL2Rβγ with reduced binding to CD25, on Tregs, effector NK cells, and T-cell subsets. Unlike wild-type (WT) IL2, F42K did not efficiently induce the expansion of highly suppressive ICOS<sup>+</sup> Tregs in peripheral blood mononuclear cells (PBMC) from healthy controls and melanoma patients. Instead, it promoted the expansion of CD16<sup>+</sup>CD56<sup>+</sup> NK cells and CD56<sup>hi</sup>CD16<sup>-</sup> NK cell subsets in both short- and long-term cultures, with enhanced Bcl-2 expression. Stimulation of PBMCs with F42K induced expression of more NK cell activation molecules, such as NKp30, NKp44, DNAM-1, NKG2D, 4-1BB/CD137, and Tim-3, than WT IL2. F42K induced greater upregulation of TRAIL, and NK-mediated cytolytic activity was increased against both autologous and HLA-mismatched melanoma cells compared with WT IL2. Gene expression analysis revealed distinct gene expression profiles stimulated by F42K, WT IL2, and IL15. F42K therapy in vivo also induced a dramatic reduction in the expansion of ICOS<sup>+</sup> Tregs, promoted NK cell expansion, and inhibited melanoma tumor growth more efficiently than WT IL2 and more effectively than anti-CTLA-4. Our findings suggest that F42K could be a potential substitute for WT IL2 as a cytokine therapy for cancer. Cancer Immunol Res; 4(11); 983-94. ©2016 AACR.
A model based approach to predict stream travel time using public transit as probes (2011)

Travel time is one of the most preferred traffic information by a wide variety of travelers. Travel time information provided through variable message signs at the roadside could be viewed as a traffic management strategy designed to encourage drivers to take an alternate route. At the same time, it could also be viewed as a traveler information service designed to ensure that the driver has the best available information based on which they can make travel decisions. In an Intelligent Transportation Systems (ITS) context, both the Advanced Traveler Information Systems (ATIS) and the Advance Traffic Management Systems (ATMS) rely on accurate travel time prediction along arterials or freeways. In India, currently there is no permanent system of active test vehicles or license plate matching techniques to measure stream travel time in urban arterials. However, the public transit vehicles are being equipped with Global Positioning System (GPS) devices in major metropolitan cities of India for providing the bus arrival time information at bus stops. However, equipping private vehicles with GPS to enable the stream travel time measurement is difficult due to the requirement of public participation. The use of the GPS equipped buses as probe vehicles and estimating the stream travel time is a possible solution to this problem. The use of public transit as probes for travel time estimation offers advantages like frequent trips during peak hours, wide range network coverage, etc. However, the travel time characteristics of public transit buses are influenced by the transit characteristics like frequent acceleration, deceleration and stops due to bus stops besides their physical characteristics. Also, the sample size of public transit is less when compared to the total vehicle population. Thus mapping the bus travel time to stream travel time is a real challenge and this difficulty is more complex in traffic conditions like in India with its heterogeneity and lack of lane discipline. As a pilot study, a model based approach using the Kalman filtering technique to predict stream travel time from public transit is carried out in the present study. Since it is only a pilot study, only twowheeled vehicles have been considered as they constitute a major proportion in the study area. The prediction scheme is corroborated using field data collected by carrying GPS units in two-wheelers traveling along with the buses under consideration. The travel time estimates from the model were compared with the manually observed travel times and the results are encouraging.
Separation and analysis of human chromosomes by combined velocity sedimentation and flow sorting applying single‐ and dual‐laser flow cytometry (1984)

Human chromosomes were separated on basis of size by velocity sedimentation at 52g in a specially designed sedimentation chamber. The chamber has been constructed in such a way that large numbers of chromosomes can be fractionated on a sucrose gradient while wall sedimentation, streaming, and swirling movements of the gradient during centrifugation are eliminated. Flow deflectors in the chamber allow undisturbed introduction and fractionation of the density gradient. The different chromosomal fractions obtained are highly enriched for the various human chromosomes. Individual chromosomes were subsequently sorted to purity by fluorescence activated flow sorting using a FACS IV flow sorter equipped with a 4-W argon-ion laser. Following this procedure, the sorting rate for specific chromosomes can be speeded up by a factor of 5-10 when the preenriched chromosomal fractions are used as starting material. A high chromosome resolution could be obtained by a few simple modifications of the FACS IV. By applying fluorochromes with different DNA-base specificity the sorting possibilities of individual human chromosomes can be improved. In addition, the chromosomal fractions were analysed by dual-laser flow cytometry after staining the chromosomes with Hoechst 33258 and chromomycin A3. In this way the enrichment of virtually all individual human chromosomes in the different chromosomal fractions can be visualized.
Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase – engineering a thermostable ATP independent enzyme (2012)

Mutational analysis of the heat stable RNA ligase from Methanobacterium thermoautotrophicum resulted in the creation of an ATP independent ligase. The K97A mutant is defective in the first two steps of ligation but retains full activity in ligation of either RNA or ssDNA to a pre-adenylated linker. The ability of the ligase to function at 65°C should reduce the constraints of RNA secondary structure in RNA ligation experiments.
Zinc: a perinatal point of view. (1993)

Zinc is a metal with great nutritional importance, particularly during periods of rapid growth due to its intervention in cellular replication as well as in the development of the immune response. A review of studies by several authors trying to determine the normal behaviour of zinc during pregnancy and early infancy is done, in an attempt to establish adequate circulating levels, in order to be able to identify zinc deficiency correctly and provide appropriate treatment. However, the information is very contradictory and no real conclusion can be reached.
Metformin Increases Mitochondrial Energy Formation in L6 Muscle Cell Cultures (2013)

A popular hypothesis for the action of metformin, the widely used anti-diabetes drug, is the inhibition of mitochondrial respiration, specifically at complex I. This is consistent with metformin stimulation of glucose uptake by muscle and inhibition of gluconeogenesis by liver. Yet, mitochondrial inhibition is inconsistent with metformin stimulation of fatty acid oxidation in both tissues. In this study, we measured mitochondrial energy production in intact cells adapting an in vivo technique of phosphocreatine (PCr) formation following energy interruption ("PCr recovery") to cell cultures. Metformin increased PCr recovery from either dinitrophenol (DNP) or azide in L6 cells. We found that metformin alone had no effect on cell viability as measured by total ATP concentration, trypan blue exclusion, or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction. However, treatments with low concentrations of DNP or azide reversibly decreased ATP concentration. Metformin increased 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction during recovery from either agent. Viability measured by trypan blue exclusion indicated that cells were intact under these conditions. We also found that metformin increased free AMP and, to a smaller extent, free ADP concentrations in cells, an action that was duplicated by a structurally unrelated AMP deaminase inhibitor. We conclude that, in intact cells, metformin can lead to a stimulation of energy formation, rather than an inhibition.
Calorigenic effect of diiodothyronines in the rat. (1996)

1. In hypothyroid rats, we determined the effects of administration of different doses of 3,3',5-triiodo-L-thyronine (T3), 3,3'-diiodo-L-thyronine (3,3'-T2) and 3,5-diiodo-L-thyronine (3,5-T2) ("T2 isomers' refers specifically to these latter two isomers throughout this paper) on resting metabolism (RM) and on the oxidative capacity (measured as cytochrome oxidase activity) of tissues that are metabolically very active. 2. The T2 isomers induced a dose-dependent calorigenic effect when injected I.P. into hypothyroid rats. The increase in RM was already evident at a dose of 2.5 micrograms (100 g body wt)(-1), and the greatest effect was observed at the highest dose, 10 micrograms (100 g body wt)(-1), when RM reached a value not significantly different from that of the euthyroid controls (1.92 +/- 0.08 and 1.93 +/- 0.13 (1 O2) kg(-1) h(-1) for 3,5'-T2, respectively, vs. 2.1 +/- 0.12 (1 O2) kg(-1) h(-1) for euthyroid controls). T3 administration restored RM to normal euthyroid values, even at a dose of 2.5 micrograms (100 g body wt)(-1). 3. The effect of T2 isomers on RM was paralleled by an increase in the oxidative capacity of tissues that are metabolically very active (liver, skeletal muscle, brown adipose tissue (BAT) and heart). The increases were between 33% (liver + 3,3'-T2) and 63% (muscle + 3,3'-T2). By contrast, T3 induced its greatest effect on the liver, with a smaller effect on skeletal muscle, but no significant stimulation in heart and BAT, whatever the dose. 4. These results suggest that T8 isomers might be mediators of the direct thyroid hormone regulation of energy metabolism.
Two-photon optical beam induced current imaging through the backside of integrated circuits (1997)

Two-photon optical beam induced current (TOBIC) images were acquired through the polished backsides of integrated circuits. An excitation beam with a photon energy below the band gap can traverse even thick substrates virtually unattenuated. At the focus—and only there—two-photon absorption generates electron-hole pairs very efficiently when using a sub-picosecond light source. An additional advantage of TOBIC is a significant increase in spatial resolution. With high numerical aperture objective lense features smaller than 1 μm are easily discernible.
Synthesis of 6-benzyl-3-oxo-6-azabicyclo[3.2.1]octane (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 6-benzyl-3-oxo-6-azabicyclo[3.2.1]octaneWalter J. Gensler, Christos D. Gatsonis, and Quazi A. AhmedCite this: J. Org. Chem. 1968, 33, 7, 2968–2971Publication Date (Print):May 1, 1968Publication History Published online1 May 2002Published inissue 1 May 1968https://doi.org/10.1021/jo01271a082RIGHTS & PERMISSIONSArticle Views55Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (572 KB) Get e-Alerts Get e-Alerts
Violating rotating camera geometry: the effect of radial distortion on self-calibration (2002)

We show that radial distortion of images invalidates the geometric constraint on which self-calibration of a rotating camera is based on, that is, 3D lines drawn between matched features all intersect at the rotation centre. We develop a geometric picture showing how radial distortion violates this constraint and discuss the implications for self-calibration of a rotating camera. In particular we show that the behaviour of self-calibration is markedly different for pin-cushion and barrel distortion, the latter causing self-calibration to be unreliable or to fail completely. A method is presented for automatically estimating the radial distortion over a sequence of images, when both distortion and camera internal parameters vary. We discuss when such an approach will work and whether accurate automatic calibration of a rotating camera is really possible.
Expression of Epithelial Cell Adhesion Molecule (EpCam) in Renal Epithelial Tumors (2004)

EpCam is an epithelial adhesion molecule expressed in a broad range of carcinomas. Clinical trials with specific humanized anti-EpCam antibodies have shown promising results and have been inaugurated in renal cell carcinoma (RCC) therapy. To study the EpCam expression profile, primary renal cell neoplasms as well as corresponding metastases were evaluated by immunohistochemistry in tissue microarrays. EpCam expression in oncocytomas and chromophobe RCCs was determined on conventional large sections. Moderate or strong EpCam expression was found in eighteen percent of clear cell (n=147), 75% of chromophobe (n=12), and 55% of papillary RCCs (n=20), but not in oncocytomas (n=3). On large sections, 90% of chromophobe RCCs (n=20) showed a strong and homogeneous positivity, whereas oncocytomas (n=15) revealed EpCam positivity in single tumor cells or small clusters. Fourteen percent of RCC metastases (n=97) showed EpCam expression. Patients with EpCam expressing clear cell RCC showed a trend toward a better prognosis in a Cox regression analysis including stage, grade, and necrosis. The data suggest EpCam as a potential therapeutic target in a subset of patients with RCC. In addition, expression patterns of EpCam could become a helpful tool in the discrimination of chromophobe RCC and oncocytoma.
Brain somatostatin: a candidate inhibitory role in seizures and epileptogenesis (1999)

Recent evidence shows that neuropeptide expression in the CNS is markedly affected by seizure activity, particularly in the limbic system. Changes in neuropeptides in specific neuronal populations depend on the type and intensity of seizures and on their chronic sequelae (i.e. neurodegeneration and spontaneous convulsions). This paper reviews the effects of seizures on somatostatin-containing neurons, somatostatin mRNA and immunoreactivity, the release of this peptide and its receptor subtypes in the CNS. Differences between kindling and status epilepticus in rats are emphasized and discussed in the light of an inhibitory role of somatostatin on hippocampal excitability. Pharmacological studies show that somatostatin affects electrophysiological properties of neurons, modulates classical neurotransmission and has anticonvulsant properties in experimental models of seizures. This peptidergic system may be an interesting target for pharmacological attempts to control pathological hyperactivity in neurons, thus providing new directions for the development of novel anticonvulsant treatments.
Adaptive signal processing for adaptive control (2005)

A few of the well established methods of adaptive signal processing are modified and extended for application to adaptive control. An unknown plant will track an input command signal if the plant is preceded by a controller whose transfer function approximates the inverse of the plant transfer function. An adaptive inverse modeling process can be used to obtain a stable controller, whether the plant is minimum or non-minimum phase. No direct feedback is involved. However the system output is monitored and utilized in order to adjust the parameters of the controller. The proposed method is a promising new approach to the design of adaptive control systems.
Mesenchymal Stem Cell Surface Antigen SB-10 Corresponds to Activated Leukocyte Cell Adhesion Molecule and Is Involved in Osteogenic Differentiation (1998)

Bone marrow contains a rare population of mesenchymal stem cells (MSCs) capable of giving rise to multiple mesodermal tissues including bone, cartilage, tendon, muscle, and fat. The cell surface antigen recognized by monoclonal antibody SB-10 is expressed on human MSCs but is lost during their developmental progression into differentiated phenotypes. Here we report on the immunopurification of the SB-10 antigen and its identification as activated leukocyte-cell adhesion molecule (ALCAM). Mass spectrometry establishes that the molecular mass of ALCAM is 80,303 +/- 193 Da and that it possesses 17,763 +/- 237 Da of N-linked oligosaccharide substituents. Molecular cloning of a full-length cDNA from a MSC expression library demonstrates nucleotide sequence identity with ALCAM. We also identified ALCAM homologs in rat, rabbit, and canine MSCs, each of which is over 90% identical to human ALCAM in their peptide sequence. The addition of antibody SB-10 Fab fragments to human MSCs undergoing osteogenic differentiation in vitro accelerated the process, thereby implicating a role for ALCAM during bone morphogenesis and adding ALCAM to the group of cell adhesion molecules involved in osteogenesis. Together, these results provide evidence that ALCAM plays a critical role in the differentiation of mesenchymal tissues in multiple species across the phylogenetic tree.
Genetically engineered foot-and-mouth disease viruses with poly(C) tracts of two nucleotides are virulent in mice (1993)

To determine the role of the poly(C) tract found at the 5' end of the genome of foot-and-mouth disease virus, synthetic RNAs (in vitro transcripts) with poly(C) tracts of different lengths have been produced and evaluated. RNAs with poly(C) tracts of 35, 25, 16, 6, or 2 residues displayed similar specific infectivities in baby hamster kidney (BHK) cells. Viruses recovered from cells transfected with in vitro transcripts containing 6 to 35 Cs had properties similar to those of the wild-type virus in cell culture, and poly(C) tracts present in the synthetic RNA-derived viruses ranged from 75 to 140 bases in length. Viruses recovered from transcripts containing only two Cs showed very different properties. Specifically, viruses grew to much lower levels in cell culture and maintained a poly(C) tract of only two residues. The pool of viruses harvested from cells transfected with the synthetic C2 RNA also contained a small amount of a virus with a 42-base deletion in the region of the poly(C) tract, which appeared to have arisen by recombination. Taken together, these data suggest that recombination provides the mechanism of poly(C) elongation and that viruses with poly(C) tracts over 75 bases in length have a selective advantage in cell culture. Interestingly, all of the in vitro transcript-derived viruses [including viruses with poly(C) tracts of only two residues] were equally virulent in mice, indicating that poly(C) tract length has no effect on virulence in this animal model.
Cardiotoxicity of Doxorubicin: Effects of Drugs Inhibiting the Release of Vasoactive Substances (1994)

The therapeutic usefulness of doxorubicin, an antineoplastic drug, is limited by its cardiotoxicity whose mechanism is as yet unknown. Several hypotheses have been postulated including also the release of vasoactive substances, so the aim of the present investigations was to study the relationship between the release of vasoactive substances and the development of doxorubicin-induced cardiotoxicity. The effects of the following drugs on doxorubicin-induced (cumulative dose of 20 mg/kg intraperitoneally) cardiotoxicity in rats have been evaluated: verapamil (1 and 10 mg/kg orally), that inhibits the slow channel influx of calcium and catecholamine release, acetylsalicylic acid (50 and 100 mg/kg orally), that inhibits the prostaglandin biosynthesis and release, and cromolyn sodium (cromolyn; 1 and 10 mg/kg orally), that inhibits the secretion of histamine. Our results showed that verapamil reduced and delayed doxorubicin-induced mortality, and limited doxorubicin-induced body weight decrease and ECG changes. Acetylsalicylic acid and cromolyn did not protect against doxorubicin-induced cardiotoxicity. These findings suggest that the release of vasoactive substances does not play a prevalent role in the development of doxorubicin-induced cardiotoxicity. The protective effect of verapamil is probably due to the inhibition of doxorubicin-induced intracellular calcium overload.
Identification of a Protein that Binds to the SH3 Region of Abl and Is Similar to Bcr and GAP-rho (1992)

A Src homology 3 (SH3) region is a sequence of approximately 50 amino acids found in many nonreceptor tyrosine kinases and other proteins. Deletion of the SH3 region from the protein encoded by the c-abl proto-oncogene activates the protein's transforming capacity, thereby suggesting the participation of the SH3 region in the negative regulation of transformation. A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially. The sequence of the 3BP-1 protein is similar to that of a COOH-terminal segment of Bcr and to guanosine triphosphatase-activating protein (GAP)-rho, which suggests that it might have GAP activity for Ras-related proteins. The 3BP-1 protein may therefore be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.