gebhart/patrepeval huggingface dataset:
Lipase-catalyzed irreversible transesterification for preparative synthesis of chiral glycerol derivatives (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLipase-catalyzed irreversible transesterification for preparative synthesis of chiral glycerol derivativesYi Fong Wang and Chi Huey WongCite this: J. Org. Chem. 1988, 53, 13, 3127–3129Publication Date (Print):June 1, 1988Publication History Published online1 May 2002Published inissue 1 June 1988https://pubs.acs.org/doi/10.1021/jo00248a046https://doi.org/10.1021/jo00248a046research-articleACS PublicationsRequest reuse permissionsArticle Views524Altmetric-Citations136LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Tumor Cell Interactions with the Extracellular Matrix During Invasion and Metastasis (1993)

Recent findings have produced great strides in developing an understanding of the molecular events involved in processes necessary for tumor cell invasion and subsequent metastasis formation. This information has been useful in developing new targets for therapeutic intervention such as disruption of tumor cell attachment by peptide analogues of cell adhesion molecules and the use of protease inhibitors to limit extracellular matrix proteolysis required for tumor cell invasion. Future efforts must focus on how the events of cell attachment, matrix proteolysis, and cell migration are controlled and integrated. This requires a better understanding of the transcriptional controls and cell signaling mechanisms that are involved in these events. Preliminary findings suggest that cell-matrix interactions influence gene expression and that the protease inhibitor balance can greatly influence cell-matrix interactions. Therefore it appears that all three steps in the invasive process are linked and interdependent. While this complicates the study of these processes, it is our belief that understanding this interdependence is critical for further development of metastasis research.
Magnetically controlled mass-loss from extrasolar planets in close orbits (2014)

We consider the role magnetic fields play in guiding and controlling mass-loss via evaporative outflows from exoplanets that experience UV irradiation. First, we present analytic results that account for planetary and stellar magnetic fields, along with mass-loss from both the star and planet. We then conduct series of numerical simulations for gas giant planets, and vary the planetary field strength, background stellar field strength, UV heating flux, and planet mass. These simulations show that the flow is magnetically controlled for moderate field strengths and even the highest UV fluxes, i.e. planetary surface fields BP ≳ 0.3 G and fluxes FUV ∼ 106 erg s−1. We thus conclude that outflows from all hot Jupiters with moderate surface fields are magnetically controlled. The inclusion of magnetic fields highly suppresses outflow from the night side of the planet. Only the magnetic field lines near the pole are open and allow outflow to occur. The fraction of open field lines depends sensitively on the strength (and geometry) of the background magnetic field from the star, along with the UV heating rate. The net effect of the magnetic field is to suppress the mass-loss rate by (approximately) an order of magnitude. Finally, some open field lines do not allow the flow to pass smoothly through the sonic point; flow along these streamlines does not reach a steady state, resulting in time-variable mass-loss.
First total synthesis of taxol. 2. Completion of the C and D rings (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFirst total synthesis of taxol. 2. Completion of the C and D ringsRobert A. Holton, Hyeong Baik Kim, Carmen Somoza, Feng Liang, Ronald J. Biediger, P. Douglas Boatman, Mitsuru Shindo, Chase C. Smith, Soekchan Kim, and Cite this: J. Am. Chem. Soc. 1994, 116, 4, 1599–1600Publication Date (Print):February 1, 1994Publication History Published online1 May 2002Published inissue 1 February 1994https://pubs.acs.org/doi/10.1021/ja00083a067https://doi.org/10.1021/ja00083a067research-articleACS PublicationsRequest reuse permissionsArticle Views9913Altmetric-Citations450LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
An optical FDM-add/drop multiplexing ring network utilizing fiber Fabry-Perot filters and optical circulators (1993)

An optical ring network based on optical frequency division multiplexing (OFDM) and add/drop multiplexers (ADMs) that use fiber Fabry-Perot (FFP) filters and optical circulators is proposed for a logical star network. In order to verify the feasibility of the ring network, 100-GHz-spaced, three-channel intensity-modulated direct detection (IM-DD) transmission is demonstrated at a bit rate of 622 Mb/s. Taking interchannel crosstalk into account, the maximum number of nodes is estimated to be 30 if the FFP filters have a finesse of 100.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Validation and comparison of indexes for fibrosis and cirrhosis prediction in chronic hepatitis C patients: proposal for a pragmatic approach classification without liver biopsies (2006)

Noninvasive indexes have been developed to predict fibrosis staging. The aim of this study was to assess the diagnostic accuracy of these indexes in comparison with liver histology in hepatitis C virus (HCV)-infected patients. A total of 235 consecutive patients with HCV infection from the Fibropaca multicentre independent study were included in this paper. FibroTest (FT), aspartate aminotransferase to platelet ratio index (APRI) and Forns score were assessed in the cohort and compared with liver histology performed on the same day. The main end point was the area under characteristic curves (AUCs) for the diagnosis of significant fibrosis (F2-F4) and cirrhosis (F4) by the METAVIR classification. Mean age was 46 (+/-11) years, 55% were males, 42% (n = 99) had significant fibrosis (F2-F4) and 7% (n = 16) had cirrhosis (F4). For the diagnosis of significant fibrosis, respective AUCs of FT, APRI and Forns score were 0.81 (95% confidence interval: 0.76-0.86), 0.71 (0.67-0.79) and 0.76 (0.70-0.82); for cirrhosis prognosis, AUCs of FT and APRI were 0.82 (0.77-0.87) and 0.81 (0.76-0.86) (AUCs not significantly different). Using each index independently, all patients were classified by FT, 214 (91%) patients were classified by APRI and 129 (55%) by Forns score. There were significantly more cases of discordances between APRI and liver biopsy than between FT or Forns score and liver biopsy (P < 0.05). Performing all scores (FT, Forns and APRI) without liver biopsy allowed fibrosis to be well evaluated in 191 patients (81.3%), including patients with FT failure. Liver biopsy remained mandatory to evaluate fibrosis in 44 patients (18.7%). Our study shows that performing all the tests and liver biopsy improves the diagnostic accuracy for liver fibrosis in chronic hepatitis C patients without patent comorbidities. The combination of all tests with liver biopsy allowed 225/235 (96%) patients to be correctly classified. The combination of all tests without liver biopsy allowed 191/235 (81.3%) patients to be correctly classified; liver biopsy remained mandatory in some patients (18.7%).
In-solution hybrid capture of bisulfite-converted DNA for targeted bisulfite sequencing of 174 ADME genes (2013)

DNA methylation is one of the most important epigenetic alterations involved in the control of gene expression. Bisulfite sequencing of genomic DNA is currently the only method to study DNA methylation patterns at single-nucleotide resolution. Hence, next-generation sequencing of bisulfite-converted DNA is the method of choice to investigate DNA methylation profiles at the genome-wide scale. Nevertheless, whole genome sequencing for analysis of human methylomes is expensive, and a method for targeted gene analysis would provide a good alternative in many cases where the primary interest is restricted to a set of genes. Here, we report the successful use of a custom Agilent SureSelect Target Enrichment system for the hybrid capture of bisulfite-converted DNA. We prepared bisulfite-converted next-generation sequencing libraries, which are enriched for the coding and regulatory regions of 174 ADME genes (i.e. genes involved in the metabolism and distribution of drugs). Sequencing of these libraries on Illumina's HiSeq2000 revealed that the method allows a reliable quantification of methylation levels of CpG sites in the selected genes, and validation of the method using pyrosequencing and the Illumina 450K methylation BeadChips revealed good concordance.
Balance of inflammatory response in stable gingivitis and progressive periodontitis lesions (2006)

The balance between inflammatory mediators and their counter-regulatory molecules may be crucial for determining the outcome of immune pathology of periodontal diseases. Based on clinical and immunological findings, the immune response in stable gingivitis lesion is supposed to be in balance, whereas the response is skewed towards the predominance of proinflammatory reactivity in progressive periodontitis lesion. However, this hypothesis has not been verified. Therefore, the aim of this study was to compare the gene expression profile of inflammatory mediators including proinflammatory cytokines and other inflammatory molecules, and anti-inflammatory cytokines by using quantitative real-time polymerase chain reaction in gingivitis and periodontitis lesions showing distinct clinical entities. For inflammatory mediators, interleukin (IL)-1beta, interferon (IFN)-gamma and receptor activator of nuclear factor (NF)-kappaB ligand tended to be higher in periodontitis, whereas tumour necrosis factor (TNF)-alpha and IL-12 p40 showed no difference. Heat-shock protein 60 (HSP60) expression was up-regulated significantly in periodontitis. For anti-inflammatory cytokines, transforming growth factor (TGF)-beta1 expression tended to be higher in periodontitis compared with gingivitis, whereas no difference was observed for IL-10 and IL-4. These findings support further our previous finding that autoimmune response to HSP60 may exert in periodontitis lesion, and suggest that perhaps subtle differences in the balance of cytokines may result in different disease expression.
Comparative Fitness of Multi-Dideoxynucleoside-Resistant Human Immunodeficiency Virus Type 1 (HIV-1) in an In Vitro Competitive HIV-1 Replication Assay (1999)

We examined whether human immunodeficiency virus type 1 (HIV-1) fitness was altered upon the acquisition of a set or subset of five mutations (A62V, V75I, F77L, F116Y, and Q151M) in the pol gene, which confers resistance to multiple dideoxynucleosides (MDR), as well as the zidovudine resistance-associated mutation T215Y, using a competitive HIV-1 replication assay in a setting of an HXB2D genetic background. Target H9 cells were exposed to a 50:50 mixture of paired infectious molecular clones, and HIV-1 in the culture supernatant was transmitted to new cultures every 7 to 10 days. The polymerase-encoding region of the virus was sequenced at various time points, and the relative proportion of the two viral populations was determined. In the absence of drugs, the comparative order for replicative fitness was HIV-162/75/77/116/151 > HIV-177/116/151 > HIV-1151 > wild-type HIV-1 (HIV-1wt) > HIV-175/77/116/151 > HIV-1151/215 > HIV-1215. In the presence of zidovudine or didanosine, the order was HIV-162/75/77/116/151 > HIV-177/116/151 > HIV-175/77/116/151 > HIV-1151 > HIV-1215. HIV-1215S(TCC), a putative intermediate infectious clone for HIV-1215, replicated comparably to HIV-1wt, while two putative intermediates for HIV-1151 [HIV-1151L(CTG) and HIV-1151K(AAG)] replicated much less efficiently than HIV-1wt and HIV-1151, suggesting that for HIV-1151 to develop, two base substitutions are likely to occur concurrently or within a short interval. These data may illustrate the molecular basis by which HIV-1151 emerges much less frequently than HIV-1215. The present data also demonstrate that several MDR HIV-1 variants are more fit than HIV-1wt in the absence of drugs and that resistance-associated mutations and drug pressure are critical variates for HIV-1 fitness.
Temperature insensitive arrayed waveguide gratings on InP substrates (1998)

A temperature insensitive arrayed waveguide grating (TI-AWG) with an InGaAsP-InP material system is proposed. Less than 0.1 /spl Aring///spl deg/C temperature dependence for all eight channels was achieved for the first time even though we used the InGaAsP-InP material system. This novel characteristic is in good agreement with the design principle of the TI-AWG.
On wrapping query languages and efficient XML integration (2000)

Modern applications (Web portals, digital libraries, etc.) require integrated access to various information sources (from traditional DBMS to semistructured Web repositories), fast deployment and low maintenance cost in a rapidly evolving environment. Because of its flexibility, there is an increasing interest in using XML as a middleware model for such applications. XML enables fast wrapping and declarative integration. However, query processing in XML-based integration systems is still penalized by the lack of an algebra with adequate optimization properties and the difficulty to understand source query capabilities. In this paper, we propose an algebraic approach to support efficient XML query evaluation. We define a general purpose algebra suitable for semistructured on XML query languages. We show how this algebra can be used, with appropriate type information, to also wrap more structured query languages such as OQL or SQL. Finally, we develop new optimization techniques for XML-based integration systems.
Noninvasive Imaging of Protein-Protein Interactions in Living Animals (2003)

Protein—protein interactions are fundamental to living systems. Dynamic association and dissociation of protein complexes control most cellular functions, including cell cycle progression, signal transduction, and metabolic pathways. Although complexes of proteins form within the context of a cell, protein—protein interactions regulate signals that affect overall patterns of development, normal physiology, and pathophysiology in living animals. For example, homo-and heterodimers of different homeobox proteins control limb bud and craniofacial development (1), and interactions among various cell surface receptors, scaffold proteins, and transcription factors regulate activation and trafficking of immune cells (2). Complexes of transcription factors, co-repressors, and chromatin-binding proteins maintain normal cells in a quiescent state, and disruption of these protein interactions may be significant in permitting unregulated growth of cancer cells (3). Additionally, protein interactions in signaling pathways are emerging as important therapeutic targets for cancer and other human diseases (reviewed in ref. 4). These and other similar pathways of protein interactions in specific tissues produce regional effects that cannot be investigated fully with in vitro systems.
Semantics and implementation of continuous sliding window queries over data streams (2009)

In recent years the processing of continuous queries over potentially infinite data streams has attracted a lot of research attention. We observed that the majority of work addresses individual stream operations and system-related issues rather than the development of a general-purpose basis for stream processing systems. Furthermore, example continuous queries are often formulated in some declarative query language without specifying the underlying semantics precisely enough. To overcome these deficiencies, this article presents a consistent and powerful operator algebra for data streams which ensures that continuous queries have well-defined, deterministic results. In analogy to traditional database systems, we distinguish between a logical and a physical operator algebra. While the logical algebra specifies the semantics of the individual operators in a descriptive but concrete way over temporal multisets, the physical algebra provides efficient implementations in the form of stream-to-stream operators. By adapting and enhancing research from temporal databases to meet the challenging requirements in streaming applications, we are able to carry over the conventional transformation rules from relational databases to stream processing. For this reason, our approach not only makes it possible to express continuous queries with a sound semantics, but also provides a solid foundation for query optimization, one of the major research topics in the stream community. Since this article seamlessly explains the steps from query formulation to query execution, it outlines the innovative features and operational functionality implemented in our state-of-the-art stream processing infrastructure.
Consensus Workshop on the Toxic Effects of Long-term PUVA Therapy (1998)

The possibility that there is an increased risk of melanoma in patients with psoriasis treated with psoralen-UV-A (PUVA) therapy has raised concern on the part of physicians and patients about the long-term safety of this treatment. In response to this concern, the National Psoriasis Foundation sponsored a workshop at which invited participants with expertise in PUVA therapy, psoriasis treatment, melanoma and nonmelanoma skin cancer, and epidemiological and clinical trials were asked to develop a consensus on the following 3 issues: the risk of long-term adverse effects of PUVA therapy with emphasis on nonmelanoma and melanoma skin cancer; the guidelines for physicians and patients for selection and use of PUVA therapy with consideration of the risk-benefit ratio of this treatment compared with the risk-benefit ratios of alternative treatments; and the directions for further evaluation of the long-term effects Of PUVA therapy.
Injectable preformed scaffolds with shape-memory properties (2012)

Injectable biomaterials are increasingly being explored to minimize risks and complications associated with surgical implantation. We describe a strategy for delivery via conventional needle-syringe injection of large preformed macroporous scaffolds with well-defined properties. Injectable 3D scaffolds, in the form of elastic sponge-like matrices, were prepared by environmentally friendly cryotropic gelation of a naturally sourced polymer. Cryogels with shape-memory properties may be molded to a variety of shapes and sizes, and may be optionally loaded with therapeutic agents or cells. These scaffolds have the capability to withstand reversible deformations at over 90% strain level, and a rapid volumetric recovery allows the structurally defined scaffolds to be injected through a small-bore needle with nearly complete geometric restoration once delivered. These gels demonstrated long-term release of biomolecules in vivo. Furthermore, cryogels impregnated with bioluminescent reporter cells provided enhanced survival, higher local retention, and extended engraftment of transplanted cells at the injection site compared with a standard injection technique. These injectable scaffolds show great promise for various biomedical applications, including cell therapies.
A family of poly(U) polymerases (2007)

The GLD-2 family of poly(A) polymerases add successive AMP monomers to the 3' end of specific RNAs, forming a poly(A) tail. Here, we identify a new group of GLD-2-related nucleotidyl transferases from Arabidopsis, Schizosaccharomyces pombe, Caenorhabditis elegans, and humans. Like GLD-2, these enzymes are template independent and add nucleotides to the 3' end of an RNA substrate. However, these new enzymes, which we refer to as poly(U) polymerases, add poly(U) rather than poly(A) to their RNA substrates.
QSAR study on H3-receptor affinity of benzothiazole derivatives of thioperamide. (1994)

Starting from the structure of thioperamide, a known H3-antagonist, a new series of compounds with a benzothiazole nucleus instead of the cyclohexylcarbothioamide moiety was synthesized. Various substituents, selected by experimental design, were introduced in position 6 of the benzothiazole nucleus, in order to change its physico-chemical characteristics. The lipophilicity of the synthesized compounds was measured by means of RP-HPLC, and their H3-receptor affinity was evaluated by competitive binding assays on rat cortex synaptosomes, with the labelled ligand N alpha-[3H]methylhistamine. A QSAR analysis was performed on the experimental data, using also substituent constants taken from the literature. The newly synthesized compounds showed lower H3-affinities than thioperamide; quantitative structure-activity relationships, described by models obtained with PLS and MRA techniques, were observed among benzothiazole derivatives. According to these relationships, any attempt to improve the potency of these compounds should involve the substitution of the benzothiazole moiety with less bulky and/or more flexible structures, which should also be less lipophilic and allow better electronic interactions with the binding site. 1-(Benzothiazol-2-yl)-4-[(1H)-imidazol-4-yl]piperidine represents a limit structure for H3-activity, since it seems impossible to improve its affinity by means of substitution in the studied position of the benzothiazole nucleus, as shown by predictions performed by a PLS model.