gebhart/patrepeval huggingface dataset:
Comparison of methodological uncertainties within permeability measurements (2007)

Abstract Permeability measurements are critical to the calculation of water‐flow within hillslopes. Despite this, errors in permeability measurements are often ignored, and can be very large particularly in disturbance‐sensitive gley soils. This work compares the uncertainties associated with six field methods of permeametry applied to a gleyed soil in upland Britain. Slug tests, constant‐head borehole permeametry, and falling‐head borehole permeametry were undertaken on established piezometers. Additionally, ring permeametry and two types of trench tests were evaluated. Method‐related uncertainty due to proximity of impeding layers of high sorptivity soils produces under‐ and over‐estimates of permeability by a factor of up to 0·2 and 5, respectively. This uncertainty band is smaller than the observed effects of anisotropy and temporal variability. Had smearing and soil‐ring leakage errors not been minimized, the methodological uncertainties would have been so large that they would have distorted the true spatial field of permeability and its estimated impact on the balance of vertical and lateral flow. Copyright © 2007 John Wiley &amp; Sons, Ltd.
Activation of Gibberellin Biosynthesis and Response Pathways by Low Temperature during Imbibition of Arabidopsis thaliana Seeds[W] (2004)

Exposure of imbibed seeds to low temperature (typically 4 degrees C) is widely used to break seed dormancy and to improve the frequency of germination. However, the mechanism by which temperature accelerates germination is largely unknown. Using DNA microarray and gas chromatography-mass spectrometry analyses, we found that a subset of gibberellin (GA) biosynthesis genes were upregulated in response to low temperature, resulting in an increase in the level of bioactive GAs and transcript abundance of GA-inducible genes in imbibed Arabidopsis thaliana seeds. Using a loss-of-function mutant, the cold-inducible GA biosynthesis gene, AtGA3ox1, was shown to play an essential role in mediating the effect of low temperature. Besides temperature, AtGA3ox1 also is positively regulated by active phytochrome and negatively regulated by GA activity. We show that both red light and GA deficiency act in addition to low temperature to elevate the level of AtGA3ox1 transcript, indicating that multiple signals are integrated by the AtGA3ox1 gene to control seed germination. When induced by low temperature, AtGA3ox1 mRNA was detectable by in situ RNA hybridization in an additional set of cell types relative to that in red light-induced seeds. Our results illustrate that the GA biosynthesis and response pathways are activated during seed imbibition at low temperature and suggest that the cellular distribution of bioactive GAs may be altered under different light and temperature conditions.
The Active Site in Nanoparticle Gold Catalysis (2004)

Facets of nanoparticle-microbe interactions and their roles in nanobioremediation of environmental pollutants: Biochemical, molecular, and technological perspectives,
Reduced receptor editing in lupus-prone MRL/lpr mice (2007)

The initial B cell repertoire contains a considerable proportion of autoreactive specificities. The first major B cell tolerance checkpoint is at the stage of the immature B cell, where receptor editing is the primary mode of eliminating self-reactivity. The cells that emigrate from the bone marrow have a second tolerance checkpoint in the transitional compartment in the spleen. Although it is known that the second checkpoint is defective in lupus, it is not clear whether there is any breakdown in central B cell tolerance in the bone marrow. We demonstrate that receptor editing is less efficient in the lupus-prone strain MRL/lpr. In an in vitro system, when receptor-editing signals are given to bone marrow immature B cells by antiidiotype antibody or after in vivo exposure to membrane-bound self-antigen, MRL/lpr 3-83 transgenic immature B cells undergo less endogenous rearrangement and up-regulate recombination activating gene messenger RNA to a lesser extent than B10 transgenic cells. CD19, along with immunoglobulin M, is down-regulated in the bone marrow upon receptor editing, but the extent of down-regulation is fivefold less in MRL/lpr mice. Less efficient receptor editing could allow some autoreactive cells to escape from the bone marrow in lupus-prone mice, thus predisposing to autoimmunity.
A reforma da previdÃªncia tem impacto progressivo? Uma anÃ¡lise das novas alÃquotas de contribuiÃ§Ã£o (2019)

Analisamos os efeitos distributivos da reforma do sistema previdenciAirio brasileiro, especialmente das mudanA§as nas alA­quotas de contribuiA§A£o. O ponto de partida A© o texto da Proposta de Emenda A  ConstituiA§A£o 6/2019 como aprovado na CA¢mara dos Deputados. Descrevemos os gargalos do sistema atual e como as principais mudanA§as nas regras impactam os trabalhadores de hoje, os trabalhadores do futuro e o equilA­brio fiscal. ConcluA­mos que as novas alA­quotas de contribuiA§A£o sA£o mais progressivas que as vigentes. Os resultados sugerem, ainda, que a mudanA§a nas alA­quotas, tudo o mais constante, deve reduzir a desigualdade como medida pelo A­ndice de Gini. Recomenda-se que estudos futuros pesquisem esse nexo causal mais profundamente, a fim de avaliar o efeito de equilA­brio geral das mudanA§as nas alA­quotas de contribuiA§A£o e da reforma da previdAancia sobre a desigualdade. Palavras-chave : PrevidAancia social; Seguridade social; Reforma da previdAancia; PEC 6/2019.
Gland segmentation in colon histology images using hand-crafted features and convolutional neural networks (2016)

We investigate glandular structure segmentation in colon histology images as a window-based classification problem. We compare and combine methods based on fine-tuned convolutional neural networks (CNN) and hand-crafted features with support vector machines (HC-SVM). On 85 images of H&E-stained tissue, we find that fine-tuned CNN outperforms HC-SVM in gland segmentation measured by pixel-wise Jaccard and Dice indices. For HC-SVM we further observe that training a second-level window classifier on the posterior probabilities - as an output refinement - can substantially improve the segmentation performance. The final performance of HC-SVM with refinement is comparable to that of CNN. Furthermore, we show that by combining and refining the posterior probability outputs of CNN and HC-SVM together, a further performance boost is obtained.
The therapeutic effect of human recombinant macrophage colony stimulating factor (CSF-1) in experimental murine metastatic melanoma. (1989)

This paper investigates the effect of recombinant human macrophage colony-stimulating factor (CSF-1) on the interaction between mononuclear phagocytes and the metastatic murine melanoma, B16/B16. CSF-1 had no effect on the ability of primary or bone marrow-derived macrophages to kill B16 cells in vitro, nor on their activation for cytotoxicity by gamma interferon plus LPS. However, when administered in vivo, CSF-1 increased the number of monocytes and peritoneal cells in tumor-bearing animals, and led to a significant reduction in the appearance of pulmonary and extra-pulmonary metastatic lesions derived from primary B16 tumors. The results suggest a therapeutic potential for CSF-1 in the treatment of malignancy.
Involvement of the vagus nerves in the regulation of basal hepatic glucose production in conscious dogs (2002)

We determined if blocking transmission in the fibers of the vagus nerves would affect basal hepatic glucose metabolism in the 18-h-fasted conscious dog. A pancreatic clamp (somatostatin, basal portal insulin, and glucagon) was employed. A 40-min control period was followed by a 90-min test period. In one group, stainless steel cooling coils (Sham, n = 5) were perfused with a 37 degrees C solution, while in the other (Cool, n = 6), the coils were perfused with -20 degrees C solution. Vagal blockade was verified by heart rate change (80 +/- 9 to 84 +/- 14 beats/min in Sham; 98 +/- 12 to 193 +/- 22 beats/min in Cool). The arterial glucose level was kept euglycemic by glucose infusion. No change in tracer-determined glucose production occurred in Sham, whereas in Cool it dropped significantly (2.4 +/- 0.4 to 1.9 +/- 0.4 mg. kg(-1). min(-1)). Net hepatic glucose output did not change in Sham but decreased from 1.9 +/- 0.3 to 1.3 +/- 0.3 mg. kg(-1). min(-1) in the Cool group. Hepatic gluconeogenesis did not change in either group. These data suggest that vagal blockade acutely modulates hepatic glucose production by inhibiting glycogenolysis.
Effect of coactivation of tongue protrusor and retractor muscles on pharyngeal lumen and airflow in sleep apnea patients (2007)

The present study evaluated the effect of coactivation of tongue protrusors and retractors on pharyngeal patency in patients with obstructive sleep apnea. The effect of genioglossus (GG), hyoglossus (HG), and coactivation of both on nasal pressure (Pn):flow relationships was evaluated in a sleep study (SlS, n = 7) and during a propofol anesthesia study (AnS, n = 7). GG was stimulated with sublingual surface electrodes in SlS and with intramuscular electrodes in AnS, while HG was stimulated with surface electrodes in both groups. In the AnS, the cross-sectional area (CSA):Pn relationships was measured with a pharyngoscope to estimate velopharyngeal compliance . In the SlS, surface stimulation of GG had no effect on the critical pressure (Pcrit), HG increased Pcrit from 2.8 +/- 1.7 to 3.7 +/- 1.6 cmH(2)O, but coactivation lowered Pcrit to 0.2 +/- 1.9 cmH(2)O (P < 0.01 for both). In the AnS, intramuscular stimulation of GG lowered Pcrit from 2.6 +/- 1.3 to 1.0 +/- 2.8 cmH(2)O, HG increased Pcrit to 6.2 +/- 2.5 cmH(2)O (P < 0.01), and coactivation had a similar effect to that of GG (Pcrit = 1.2 +/- 2.4 cmH(2)O, P < 0.05). None of the interventions affected significantly velopharyngeal compliance. We conclude that the beneficial effect of coactivation depends on the pattern of GG fiber recruitment: although surface stimulation of GG failed to protrude the tongue, it prevented the occlusive effect of the retractor, thereby improving pharyngeal patency during coactivation. Stimulation of deeper GG fibers with intramuscular electrodes enlarged the pharynx, and coactivation had no additive effect.
Gene Therapy Using Hematopoietic Stem Cells (2000)

While gene therapy using hematopoietic stem cells was the first area of investigation in the field, success has proven elusive. However, significant progress has been achieved recently in methods for more effective gene transfer and expression. In addition to greatly improved results using retroviral vectors, adeno-associated vectors and lentiviral vectors appear to be promising for stable transduction of hematopoietic stem cells. These advances, documented in animal transplant models, are now being applied to clinical trials.
Trastuzumab Emtansine for HER2-Positive Advanced Breast Cancer (2012)

T-DM1 significantly prolonged progression-free and overall survival with less toxicity than lapatinib plus capecitabine in patients with HER2-positive advanced breast cancer previously treated with trastuzumab and a taxane. (Funded by F. Hoffmann-La Roche/Genentech; EMILIA ClinicalTrials.gov number, NCT00829166.).
泰国的人口、教育和人力政策——15—19岁年龄组案例研究 (1991)

导言随着泰国社会的工业化和现代化,泰国人口正在从高出生率向低出生率转变。这一转变具有一些基本的特点。一方面,人口出生率的逐步下降意味着需要接受义务小学教育的人数正在减少,另一方面,中学学龄人口仍然在增加。此外,中学学龄人口也是进入劳动力市场的年龄段。就这点而论,制定发展计划特别是制定人力发展计划对于泰国社会未来的福利是至关重要的。
The AD7c-NTP neuronal thread protein biomarker for detecting Alzheimer's disease* (2001)

Dementia in Alzheimer's disease (AD) is ultimately due to cell loss mediated by several mechanisms including, apoptosis, impaired mitochondrial function, and possibly necrosis. A second major neuroanatomic correlate of dementia is aberrant cortical neuritic sprouting with abundant proliferation of dystrophic neurites. Early in vivo detection of AD will require non-invasive assays of highly sensitive and relatively specific biomarkers that reflect these fundamental abnormalities in cellular function. The AD-associated neuronal thread protein (AD7c-NTP) gene encodes a approximately 41 kD membrane-spanning phosphoprotein that causes apoptosis and neuritic sprouting in transfected neuronal cells. The AD7c-NTP gene is over-expressed in AD beginning early in the course of disease. In the brain, increased AD7c-NTP immunoreactivity is associated with phospho-tau-immunoreactive cytoskeletal lesions, but not with amyloid-beta accumulations. The levels of AD7c-NTP in postmortem brain tissue correlate with the levels measured in paired ventricular fluid samples, suggesting that the protein is secreted or released by dying cells into cerebrospinal fluid (CSF). In this regard, elevated levels of AD7c-NTP can be detected in both CSF and urine of patients with early or moderately severe AD, and the CSF and urinary levels of AD7c-NTP correlate with the severity of dementia. The newest configuration of the AD7c-NTP assay, termed "7c Gold", has greater than 90% aggregate results from a number of studies suggest that AD7c-NTP is an excellent biomarker that could be helpful in the routine clinical evaluation of elderly patients at risk for AD.
Omeprazole and the Gastric Mucosa (1990)

The potential for either omeprazole or its sulphenamide to interact with DNA was investigated by incubating tritiated omeprazole at either neutral or acidic pH with either purified prokaryotic (Escherichia coli) or purified eukaryotic (salmon sperm) DNA. Each incubation was carried out for 30 min. The DNA was separated on agarose gels, stained with ethidium bromide, and the radioactivity in the DNA determined. No radioactivity was associated with the DNA on the gel with either prokaryotic or eukaryotic DNA or with either protocol. No interaction with the DNA was found, thus excluding the possibilities of base modification, DNA strand breakage or intercalation. It was concluded that neither omeprazole nor its gastric metabolites are genotoxic.
A microfabricated optically-pumped magnetic gradiometer (2017)

We report on the development of a microfabricated atomic magnetic gradiometer based on optical spectroscopy of alkali atoms in the vapor phase. The gradiometer, which operates in the spin-exchange relaxation free regime, has a length of 60 mm and cross sectional diameter of 12 mm, and consists of two chip-scale atomic magnetometers which are interrogated by a common laser light. The sensor can measure differences in magnetic fields, over a 20 mm baseline, of 10 fT/[Formula: see text] at frequencies above 20 Hz. The maximum rejection of magnetic field noise is 1000 at 10 Hz. By use of a set of compensation coils wrapped around the sensor, we also measure the sensor sensitivity at several external bias field strengths up to 150 mG. This device is useful for applications that require both sensitive gradient field information and high common-mode noise cancellation.
Real-time volumetric 3D capture of room-sized scenes for telepresence (2012)

This paper describes a 3D acquisition system capable of simultaneously capturing an entire room-sized volume with an array of commodity depth cameras and rendering it from a novel viewpoint in real-time. The system extends an existing large-scale capture system with a volumetric measurement integration and rendering engine that has been adapted for use with a fixed array of cameras. New techniques are explored to improve image quality, decrease noise, and reduce the number of required depth cameras while preserving support for a fully dynamic scene.
The e-puck, a Robot Designed for Education in Engineering (2009)

Abstract — Mobile robots have the potential to become the ideal tool to teach a broad range of engineering disciplines. Indeed, mobile robots are getting increasingly complex and accessible. They embed elements from diverse fields such as mechanics, digital electronics, automatic control, signal processing, embedded programming, and energy management. Moreover, they are attractive for students which increases their motivation to learn. However, the requirements of an effective education tool bring new constraints to robotics. This article presents the e-puck robot design, which specifically targets engineering education at university level. Thanks to its particular design, the e-puck can be used in a large spectrum of teaching activities, not strictly related to robotics. Through a systematic evaluation by the students, we show that the e-puck fits this purpose and is appreciated by 90 percent of a large sample of students. I.
Preparation and anti-HIV activity of O-acylated heparin and dermatan sulfate derivatives with low anticoagulant effect (1993)

In order to increase the ratio of anti-HIV activity to anticoagulant activity, glycosaminoglycan derivatives selectively substituted at OH and/or COOH groups were prepared. Standard heparin, heparin fragments, or dermatan sulfate were converted to their tributylammonium or tetrabutylammonium salts. Their selective O-acylation to various (controlled) degrees was carried out in a homogeneous way in N,N-dimethylformamide using carboxylic acid anhydrides and 4-(dimethylamino)pyridine as catalyst. Esterification of the COOH groups was performed by the addition of alkyl halide to an N,N-dimethylformamide solution of glycosaminoglycan tetrabutylammonium salts. The in vitro anticoagulant activity, the activity against HIV-1 and HIV-2 cytopathicity, the cytotoxicity, and the activity on the induction of giant cell formation were determined. O-acylation (O-butyrylation or O-hexanoylation) of the heparin fragments obtained by periodate depolymerization (compounds 2d and 2e), and their esters (compounds 7i and 7j), yielded products with very low anticoagulant effects in vitro, yet potent activity against both HIV-1 and HIV-2 induced cytopathicity, and low, if any, cytotoxicity. As compared to other anionic polysaccharides, these acylated derivatives are more active as inhibitors of HIV-induced giant-cell formation. Their anti-HIV activity is related to the degree of O-acylation and is mainly due to the inhibition of virus adsorption to the target cells.
Two Jasmonoid Glucosides and a Phenylvaleric Acid Glucoside fromPerilla frutescens (1996)

Three new glucosidic constituents (1–3) were isolated from the methanolie extract of Perilla frutescens. Two of these compounds (t and 2) were jasmonoid glucosides and were determined to be 5’-β-D-glueopyranosyloxyjasmonic acid and 3-β-D-glucopyranosyl-3-epi-2-isocucurbic acid, respectively. The other one (3) was a new glucoside of phenylvaleric acid that was elucidated to be 3-β-D-glucopyranosyloxy-5-phenylvaleric acid. Its aglycone was also found for the first time in nature.
In vitro and in vivo studies of antagonistic effects of human growth hormone analogs. (1994)

A bovine growth hormone (bGH) analog, bGH-G119R, has been shown to act as a functional antagonist of GH activity both in vitro and in vivo. In the present study, human (h) GH analogs with alterations in the third alpha-helix (G120A, G120R) and N terminus (I4A) were generated. These two regions have been reported to form binding site 2 in hGH, which is involved in in vitro dimerization of the GH receptor (GHR). The biological activities of these hGH analogs were tested in vitro by a radioreceptor assay and an assay to test the ability of the molecules to induce tyrosine phosphorylation of a 93-kDa protein in a human lymphocyte line, IM-9. The growth rate of transgenic mice that express different hGH analogs was used as an in vivo test of the activity of the molecules. The results demonstrated that G120R is a potent hGH antagonist both in vitro and in vivo. Transgenic mice expressing G120R demonstrated a growth-suppressed phenotype. However, I4A, which has been demonstrated to be a potent inhibitor of in vitro GHR dimerization, exhibited full growth promoting activity in transgenic mice. Thus, the ability of hGH analogs to induce GHR dimerization in vitro and the ability to promote growth in vivo are not directly correlated.
Prenatal Sex Determination by DNA Amplification from Maternal Peripheral Blood (1990)

D. LO, Y. -M.; WAINSCOAT, J. S.; GILLMER, M. D. G.; PATEL, P.; SAMPIETRO, M.; FLEMING, K. A. Author Information
Synthesis of biologically active deletion mutants of human factor VIII:C. (1988)

The recent cloning and sequence analysis of human factor VIII:C (antihaemophilic factor) revealed a domain structure for the protein which can be presented as A1-A2-B-A3-C1-C2. In this report we describe the construction of two altered factor VIII:C cDNAs coding for molecules in which a part (amino acids 816 to 1598) or all of the B domain (amino acids 741 to 1689) was removed. In the latter mutant a new thrombin cleavage site has been created, which does not exist in wild-type factor VIII:C. The mutated cDNAs were cloned into eucaryotic expression vectors based on regulatory sequences of the virus SV40 and transfected into two different mammalian cell lines. Both truncated recombinant factor VIII:C molecules were secreted into the culture medium, showed full biological activity and could be activated by thrombin.
Low levels of deoxynucleotides in peripheral blood lymphocytes: a strategy to inhibit human immunodeficiency virus type 1 replication. (1993)

Human immunodeficiency virus type 1 (HIV-1) viral DNA synthesis in quiescent and activated peripheral blood lymphocytes (PBLs) was studied. Incomplete viral DNA (previously demonstrated to be associated with HIV-1 virions) is carried by HIV-1 virions into quiescent and activated PBLs, contributing to the formation of an early viral DNA pool in these cells. The viral DNA is subsequently completed but only extremely slowly and inefficiently in quiescent PBLs compared to that in stimulated PBLs. We find that this correlates with significantly lower levels of dNTP substrates in quiescent compared to activated PBLs. At these low dNTP concentrations, HIV-1 reverse transcriptase acts in a partially distributive manner. Increasing dNTP concentrations from the levels of quiescent PBLs to the levels of activated PBLs increases the processive action of reverse transcriptase, which in turn stimulates rapid and efficient formation of full-length DNA. Furthermore, hydroxyurea treatment of stimulated PBLs decreases the dNTP levels and the DNA synthesis rate to levels comparable to quiescent PBLs. Our data therefore indicate that low levels of dNTP may explain why HIV-1 DNA is synthesized slowly and inefficiently in quiescent PBLs and suggest that pharmacologic induction of low dNTP levels represents a therapeutic approach for inhibition of HIV-1 replication.
Rac1 Activity Is Required for the Activation of Hypoxia-inducible Factor 1 (2001)

Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that mediates cellular and systemic homeostatic responses (including erythropoiesis, angiogenesis, and glycolysis) to reduced O(2) availability in mammals. Hypoxia induces both the protein expression and transcriptional activity of the HIF-1alpha subunit. However, the molecular mechanisms of sensing and signal transduction by which changes in O(2) concentration result in changes in HIF-1 activity are poorly understood. We report here that the small GTPase Rac1 is activated in response to hypoxia and is required for the induction of HIF-1alpha protein expression and transcriptional activity in hypoxic cells.