gebhart/patrepeval huggingface dataset:
Replication Studies Using Genotype 1a Subgenomic Hepatitis C Virus Replicons (2003)

Recently, cell-based replicon systems for hepatitis C virus (HCV), in which the nonstructural proteins stably replicate subgenomic viral RNA in Huh7 cells, were developed. To date, one limitation of using these replicon systems to advance drug discovery is the inability of other genotypic derivatives, beyond those of two distinct strains of genotype 1b (HCV-N and Con1), to stably replicate in Huh7 cells. In this report, we evaluated a series of replicon genotype 1a-1b chimeras, as well as a complete genotype 1a replicon clone. A subgenomic replicon construct containing only type 1a sequences failed to generate stable colonies in Huh7 cells even after repeated attempts. Furthermore, addition of an NS5A adaptive mutation (S2204I) which enhances type 1b replicon efficiency was insufficient to confer replication to the wild-type 1a replicon. This subgenomic replicon was subsequently found to be inefficiently translated in Huh7 cells compared to a type 1b replicon, and the attenuation of translation mapped to the N-terminal region of NS3. Therefore, to ensure efficient translation and thereby support replication of the 1a genome, the coding sequence for first 75 residues from type 1a were replaced with the type 1b (strain Con 1) NS3 coding sequence. Although nonstructural proteins were expressed at lower levels with this replicon than with type 1b and although the amount of viral RNA was also severalfold lower (150 copies of positive-strand RNA per cell), the replicon stably replicated in Huh7 cells. Notwithstanding this difference, the ratio of positive- to negative-strand RNA of 26 was similar to that found with the type 1b replicon. Similar results were found for a 1b replicon expressing the type 1a RNA-dependent RNA polymerase. These 1a hybrid replicons maintained sensitivity to alpha interferon (IFN-alpha), albeit with an eightfold-higher 50% inhibitory concentration than type 1b replicons. Evidence is provided herein to confirm that this differential response to IFN-alpha may be attributed directly to the type 1a polymerase.
WHY THE BOOM IN COPPER-CORE PLUGS? (1984)

This article describes copper-core spark plugs: the insulator nose is longer to reduce fouling at low speeds, and center electrode contains copper, which dissipates heat better at high speeds. All the major suppliers have copper-core spark plugs, and they are original equipment in many new cars.
Precisely Engineered Colloidal Nanoparticles and Nanocrystals for Li-Ion and Na-Ion Batteries: Model Systems or Practical Solutions? (2014)

In recent years, the search for new electrode materials for rechargeable Li-ion batteries has undergone a drastic shift toward nanomaterials. A similar tendency is expected to occur for the conceptually similar Na-ion batteries. Due to very short internal diffusion paths, nanoscale materials are far less limited by their ionic or electronic conductivities than their bulk counterparts. Nanomaterials can also withstand much greater mechanical deformation during charge/discharge cycling. Overall, these favorable effects significantly enlarge the variety of inorganic compounds that can be used as Li and Na ion storage media. Herein, we discuss the perspectives of a specific family of nanomaterials—monodisperse colloidal nanocrystals and nanoparticles—for controlling and studying the effects of size, composition, and morphology on electrochemical properties. Despite clear scientific advantages, commercialization of such nanomaterials is presently hampered by their high cost of synthesis, owing to the use of organic solvents and coordination compounds.
.alpha.-acylamino radical cyclizations: application to the synthesis of a tetracyclic substructure of gelsemine (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT.alpha.-acylamino radical cyclizations: application to the synthesis of a tetracyclic substructure of gelsemineJoong Kwon Choi, Deok Chan Ha, David J. Hart, Chih Shone Lee, Subban Ramesh, and Shung WuCite this: J. Org. Chem. 1989, 54, 2, 279–290Publication Date (Print):January 1, 1989Publication History Published online1 May 2002Published inissue 1 January 1989https://doi.org/10.1021/jo00263a006RIGHTS & PERMISSIONSArticle Views767Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A Neutralizing Anti-Nogo66 Receptor Monoclonal Antibody Reverses Inhibition of Neurite Outgrowth by Central Nervous System Myelin (2004)

The Nogo66 receptor (NgR1) is a neuronal, leucine-rich repeat (LRR) protein that binds three central nervous system (CNS) myelin proteins, Nogo, myelin-associated glycoprotein, and oligodendrocyte myelin glycoprotein, and mediates their inhibitory effects on neurite growth. Although the LRR domains on NgR1 are necessary for binding to the myelin proteins, the exact epitope(s) involved in ligand binding is unclear. Here we report the generation and detailed characterization of an anti-NgR1 monoclonal antibody, 7E11. The 7E11 monoclonal antibody blocks Nogo, myelin-associated glycoprotein, and oligodendrocyte myelin glycoprotein binding to NgR1 with IC50 values of 120, 14, and 4.5 nm, respectively, and effectively promotes neurite outgrowth of P3 rat dorsal root ganglia neurons cultured on a CNS myelin substrate. Further, we have defined the molecular epitope of 7E11 to be DNAQLR located in the third LRR domain of rat NgR1. Our data demonstrate that anti-NgR1 antibodies recognizing this epitope, such as 7E11, can neutralize CNS myelin-dependent inhibition of neurite outgrowth. Thus, specific anti-NgR1 antibodies may represent a useful therapeutic approach for promoting CNS repair after injury.
Symbolic modeling and evaluation of data paths (1995)

We present an automata model which concisely captures the constraints imposed by a data-path, such as bus hazards, register constraints, and control encoding limitations. A set of uniform base components for depicting general data-paths and techniques for systematic translation of such depictions into Boolean functions are described. Finally, this model is expanded to represent the limitations of generating as well as moving operands by incorporating data-flow graphs. The benefits of this representation are demonstrated by modeling a commercial DSP microprocessor.
IL-4-induced eosinophil accumulation in rat skin is dependent on endogenous TNF-alpha and alpha 4 integrin/VCAM-1 adhesion pathways. (1998)

IL-4 has been implicated in the pathogenesis of a number of allergic inflammatory disease states where the accumulation of eosinophils is a prominent feature. The aim of the present study was to use an isotopic in vivo model to investigate the ability of recombinant rat IL-4 in inducing eosinophil accumulation in rat skin. 111In-eosinophil accumulation in response to intradermally injected IL-4 was measured during 0 to 4 h, 24 to 28 h, and 48 to 52 h. Accumulation was detected during the first two periods, but not at the later time point. The accumulation during 24 to 28 h, which was dose dependent, was investigated in detail. Administration i.v. of an anti-rat VCAM-1 mAb, but not an anti-rat ICAM-1 mAb, inhibited the accumulation of 111In-eosinophils induced by IL-4 (maximum inhibition, 80%). Further, when the 111In-eosinophils were pretreated in vitro with an anti-beta 2 integrin mAb, an anti-alpha 4 integrin mAb, or a combination of both mAbs, before their injection into recipient rats, the IL-4-induced cell accumulation was inhibited by 63, 60, and 74%, respectively. Finally, coadministration of IL-4 with the soluble TNF receptor (p55)-IgG fusion protein significantly reduced the 111In-eosinophil accumulation induced by the cytokine, and TNF-alpha was detected in IL-4-injected skin sites by both immunostaining and bioassay. Our results demonstrate that IL-4 is a potent inducer of eosinophil accumulation in vivo, the response being dependent on the endogenous generation of TNF-alpha, beta 2 integrins, and alpha 4 integrin/VCAM-1 interactions.
Expression pattern alterations of syndecans and glypican-1 in normal and pathological trophoblast (1999)

Syndecans (syn-1, -2, -3, -4) and glypican-1 are proteoglycans expressed during development in association with changes in tissue organization and differentiation. They participate in the modulation of growth factor actions and in cell-cell and cell-matrix adhesion. The expression of syn-1, -2, -3, -4, and glypican-1 has been studied in normal human placenta and in gestational trophoblastic disease such as hydatidiform mole, invasive mole, and choriocarcinoma, using immunohistochemistry and western blots. Syndecan-3 was not expressed in normal or pathological tissues. During normal gestation, the other proteoglycans showed a specific staining pattern, which for some was modified during pregnancy. For instance, syn-1 was only expressed in syncytiotrophoblast; syn-4 was mainly localized in the villous and extravillous cytotrophoblast in the first trimester, whereas at term it was expressed in the syncytiotrophoblast. The most striking results are the altered expression patterns of syndecans and glypican-1 in pathological tissues. These proteoglycans showed a progressive decrease of immunostaining related to the increase of severity of trophoblastic disease, in particular in invasive mole and choriocarcinoma. In addition, dysregulation in the localization of the expression patterns was observed for syn-2 and -4. Because changes in syndecan expression enable cells to become more or less responsive to their micro-environment, the down-regulation and/or dysregulation of syndecans in relation to the degree of severity of trophoblastic diseases provides new insights into the progression of these pathologies.
Lamina-Specific Deficits in Parvalbumin-Immunoreactive Varicosities in the Prefrontal Cortex of Subjects With Schizophrenia: Evidence for Fewer Projections From the Thalamus (2001)

Neuronal number in the mediodorsal thalamic nucleus, the principal source of thalamic projections to the prefrontal cortex, has been reported to be lower in subjects with schizophrenia. The authors tested the hypothesis that schizophrenia is associated with a selective deficit in a marker of thalamic axon terminals in the middle layers of the prefrontal cortex, the primary zone of termination of thalamic axons.The densities of parvalbumin-immunoreactive varicosities (putative axon terminals) were determined in the superficial and middle layers of prefrontal cortex area 9 from 20 matched pairs of subjects with schizophrenia and normal comparison subjects. In order to determine the specificity of these observations, similar studies were conducted in subjects with major depressive disorder and in monkeys after 9-12 months of haloperidol treatment.The relative densities of parvalbumin-immunoreactive varicosities did not differ between schizophrenic and comparison subjects in the superficial layers. However, in the middle layers, mean varicosity density was significantly lower (24% difference) in the subjects with schizophrenia. In contrast, neither subjects with major depressive disorder nor haloperidol-treated monkeys exhibited a middle-layer density of parvalbumin-immunoreactive varicosities that was lower than that of their matched comparison groups.Although not definitive, these findings are consistent with the hypothesis of fewer projections from the mediodorsal thalamic nucleus to the prefrontal cortex in schizophrenic subjects and thus converge with other lines of evidence demonstrating an abnormality in thalamo-prefrontal cortical circuitry in persons with schizophrenia.
The Molecular Weight of Sheep Plasma Erythropoietin (1972)

The molecular weight of sheep plasma erythropoietin, measured by gel electrophoresis, in the presence of sodium dodecyl sulfate is 46,000.The sedimentation coefficient of the biologically active material is 4.6 S.
The M-PIRE MPEG-4 codec DSP and its macroblock engine (2002)

M-PIRE is a programmable MPEG-4 multimedia codec VLSI for mobile and stationary applications. It integrates a RISC core, two separate DSPs, a 64-bit dual-issue VLIW macroblock engine, and an autonomous I/O processor on a single chip to cope with the high flexibility and processing demands of the MPEG-4 standard. The first M-PIRE implementation will consume 90 mm/sup 2/ in 0.25 /spl mu/ CMOS technology. It will support real-time video and audio processing of MPEG-4 simple profile or ITU H.26x standards; future designs of M-PIRE will add support for higher MPEG-4 profiles. This paper focuses on the architecture, instruction set, and performance of M-PIRE's macroblock engine, which carries most of the workload in MPEG-4 video processing.
Treatment outcomes for primary care patients with major depression and lifetime anxiety disorders (1996)

Standardized psychotherapy and pharmacotherapy are effective for patients with major depression with and without a generalized anxiety disorder. However, the longer time to recovery for the former group and lack of response to these treatments by patients with lifetime panic disorder suggest that primary care physicians should carefully assess history of anxiety disorder among depressed patients so as to select a proper intervention.
The receptor encoded by the human C‐MET oncogene is expressed in hepatocytes, epithelial cells and solid tumors (1991)

The human c-MET oncogene encodes a transmembrane tyrosine kinase (p190c-met) with structural and functional features of a growth-factor receptor. Monoclonal antibodies (MAbs) have been used to investigate the distribution of the c-Met protein in human normal and neoplastic tissues. By immunofluorescence microscopy homogeneous expression was detected in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Positive staining was also found in epithelial cells of the endometrium and ovary, and in basal keratinocytes of esophagus and skin. By Northern blot analysis, high levels of c-met messenger RNA were detected in specimens of liver, gastro-intestinal tract and kidney. c-met-specific mRNA was also found in thyroid, pancreas and placenta, in which organs c-Met protein was barely detectable by immunofluorescence. The antibodies revealed expression of c-MET protein in hepatomas (11/14), carcinomas of colon and rectum (19/21), stomach (11/22), kidney (16/19), ovary (9/17) and skin (7/17). Carcinomas of the lung (13/20), thyroid (11/13) and pancreas (5/7) were also positive. In these last cases (lung, thyroid and pancreas) tumor cells were homogeneously stained by the antibodies, whereas in their normal counterparts staining was barely detectable. These data suggest that the receptor encoded by c-MET plays a physiological role in epithelial cell growth and that its expression is altered in human carcinomas.
Low delay LPC and MDCT-based audio coding in the EVS codec (2015)

Speech coders operating in time domain can be extended with a frequency domain mode to improve encoding of music, even though this is challenging at low delay. In such a scenario, the short analysis window limits the benefit of the transform coder, while a delayless switch between the two coders constrains the system further. The paper presents an LPC and MDCT-based audio coder part of the new 3GPP codec for Enhanced Voice Services, which aims to solve the issues. Several advanced coding tools are introduced to alleviate the constraints: transient handling is improved, harmonic structures are better preserved, and the modeling of the zero-quantized frequencies is enhanced. Test results show that the obtained low-delay switched coder brings a clear improvement over a speech coder and is competitive even in comparison to audio coders with higher delay.
Characterization of the Human Granulocyte-Macrophage Colony-Stimulating Factor Gene Promoter: an AP1 Complex and an Sp1-Related Complex Transactivate the Promoter Activity That Is Suppressed by a YY1 Complex (1996)

It is well documented that a repeated CATT element in the human granulocyte-macrophage colony-stimulating factor (GM-CSF) gene promoter is required for promoter activity. However, the transcription factors that are able to transactivate this enhancer element remain unidentified. Recently, we have found that nuclear factor YY1 can interact with the enhancer element. Here, we report that in addition to YY1, two other nuclear factors have been identified in the DNA-protein complexes formed by the CATT oligonucleotide and the Jurkat T-cell nuclear protein. One of these factors is AP1, and the other one is an Sp1-related protein. Results from transient transfection of Jurkat T cells have revealed that formation of both AP1 and the Sp1-related complex is required for the full enhancer activity of the CATT element. This result is supported by cotransfection of a c-jun expression vector and mutational analysis of the AP1 site or the Sp1-related protein binding site. In contrast, formation of the YY1 complex suppresses enhancer activity, since deletion of the YY1 complex induces an augmentation of the enhancer activity and overexpression of YY1 results in an attenuation of the enhancer activity. Results from the mechanism study have revealed that YY1 is able to inhibit transactivation mediated by either AP1 or the Sp1-related protein, and YY1 suppressive activity is DNA binding dependent. Taken together, these data support the ideas that AP1 and the Sp1-related nuclear protein are required for transactivation of the human GM-CSF gene promoter and that YY1 can suppress transactivation of the promoter even under inducible conditions.
A Carbon Fiber Implant to Aid Interbody Lumbar Fusion (1991)

A carbon-fiber-reinforced polymer implant has been designed to aid interbody lumbar fusion. The cage-like implant has ridges or teeth to resist pullout or retropulsion, struts to support weight bearing, and a hollow center for packing of autologous bone graft. Because carbon is radiolucent, bony healing can be imaged by standard radiographic techniques. The device has been mechanically tested in cadaver spines and compared with posterior lumbar interbody fusion performed with donor bone. The carbon device required a pullout force of 353 N compared with 126 N for donor bone. In compression testing, posterior lumbar interbody fusion performed with the carbon device bore a load of 5,288 N before failure of the vertebral bone. Posterior lumbar interbody fusion performed with donor bone failed at 4,628 N, and unmodified motion segments failed at 6,043 N. The carbon fiber implant separates the mechanical and biologic functions of posterior lumbar interbody fusion.
Origin and evolution of the 1918 “Spanish” influenza virus hemagglutinin gene (1999)

The "Spanish" influenza pandemic killed over 20 million people in 1918 and 1919, making it the worst infectious pandemic in history. Here, we report the complete sequence of the hemagglutinin (HA) gene of the 1918 virus. Influenza RNA for the analysis was isolated from a formalin-fixed, paraffin-embedded lung tissue sample prepared during the autopsy of a victim of the influenza pandemic in 1918. Influenza RNA was also isolated from lung tissue samples from two additional victims of the lethal 1918 influenza: one formalin-fixed, paraffin-embedded sample and one frozen sample obtained by in situ biopsy of the lung of a victim buried in permafrost since 1918. The complete coding sequence of the A/South Carolina/1/18 HA gene was obtained. The HA1 domain sequence was confirmed by using the two additional isolates (A/New York/1/18 and A/Brevig Mission/1/18). The sequences show little variation. Phylogenetic analyses suggest that the 1918 virus HA gene, although more closely related to avian strains than any other mammalian sequence, is mammalian and may have been adapting in humans before 1918.
Introduction (1998)

This chapter discusses the creation of a metaphysics of power by Hazlitt. It evaluates Hazlitt's metaphysics of power which reopened the field of enquiry outlined in ‘Romantic idealism’. It notes the ability to occupy without contradiction. Having two contradictory positions is Hazlitt's peculiar gift. The chapter states that Hazlitt's idealism is rendered unique by the form in which it is manifested, the product of a mind that is without contradiction, one which is radical and conservative all at once. His idealism evolved, not from the Germans, but from the empiricist tradition dominant in Britain. His metaphysics is the product of polemical engagement, a process of refuting and undermining the philosophies that he perceived as at once the most influential and the most invidious — the philosophies closest to home.
Local access optical networks (1996)

Both broadcast CATV and switched telephony access networks have evolved, over decades, to deliver their respective services efficiently. But these networks are not necessarily optimal for services that are rapidly emerging. This suggests that the access infrastructure will likely change in the future, driven by demands for new services and the costs of providing them. Will fiber optic networks play an important role? Trade-offs in desirable network properties (low initial costs, high capacity, flexible provisioning, enhanced maintenance and operations, etc.) are strongly influenced by the network architecture. We classify architectures on two characteristics: delivery mode (i.e., broadcast vs. switched) and carrier utilization (i.e., shared vs. dedicated), examining the trade-offs these imply with several examples of existing and proposed architectures. We show that optical networks can, by virtue of recent component development, be reconfigured to change their characteristics without changing the outside plant.
Neural Systems as Nonlinear Filters (2000)

Experimental data show that biological synapses behave quite differently from the symbolic synapses in all common artificial neural network models. Biological synapses are dynamic; their "weight" changes on a short timescale by several hundred percent in dependence of the past input to the synapse. In this article we address the question how this inherent synaptic dynamics (which should not be confused with long term learning) affects the computational power of a neural network. In particular, we analyze computations on temporal and spatiotemporal patterns, and we give a complete mathematical characterization of all filters that can be approximated by feedforward neural networks with dynamic synapses. It turns out that even with just a single hidden layer, such networks can approximate a very rich class of nonlinear filters: all filters that can be characterized by Volterra series. This result is robust with regard to various changes in the model for synaptic dynamics. Our characterization result provides for all nonlinear filters that are approximable by Volterra series a new complexity hierarchy related to the cost of implementing such filters in neural systems.