gebhart/patrepeval huggingface dataset:
Cotton rat immune responses to virus-like particles containing the pre-fusion form of respiratory syncytial virus fusion protein (2015)

VLPs containing the pre-fusion RSV F protein have characteristics required for a safe, effective RSV vaccine.
<title>Laser engraving</title> (1993)

Laser engraving device with image preprocessing is described in this paper. A pattern is imported into a computer by a scanner, then the image information is processed. According to the size required and using the density of 84 dpi, a desired picture is engraved by an axial flowing CO<SUB>2</SUB> laser beam. The objects engraved can be wood, glass, china, marble, stainless steel, etc. The maximum engraving area is 1000*1500 mm<SUP>2</SUP>.
Monoclonal antibodies against components of the IGF system for cancer treatment. (2008)

Insulin-like growth factor (IGF)-mediated signaling pathways have long been recognized as important targets for cancer therapy. The development of antibodies against the IGF type I receptor (IGF-IR) started in the early 1980s, although clinical studies of these antibodies were not conducted until more recent years. At least eight different anti-IGF-IR antibodies are currently in clinical trials and are reviewed in this article; each antibody was generated by a different method and has unique features. Information collected from these early clinical trials should be taken into consideration during the design of further studies and future clinical applications, as well as in the improvement of the respective antibodies. The development of antibodies against IGF ligands is also discussed.
Raman spectroscopy as a probe for in situ studies of pressure‐induced amorphization: some illustrative examples (2003)

Abstract The main models proposed in the literature to describe pressure‐induced amorphization (PIA) are briefly reviewed, with special emphasis on the kinetic aspects of PIA. The high potential of Raman spectroscopy for experimental studies on PIA is highlighted and illustrated by the results obtained for zirconium tungstate (ZrW 2 O 8 ) and boric acid (H 3 BO 3 ). Compared with x‐ray diffraction using a conventional x‐ray tube, Raman spectroscopy allowed a much faster identification of the transitions in both compounds. Further, the Raman results for ZrW 2 O 8 have given support to an amorphization mechanism based on the freezing of low‐energy vibrational modes associated with rigid (or quasi‐rigid) ZrO 6 and WO 4 polyhedral units. For H 3 BO 3 , we obtained strong evidence of a PIA associated with hindered decomposition of H 3 BO 3 , probably to a mixture of HBO 2 and H 2 O. The short acquisition time for the Raman measurements of samples under high pressure enabled us to follow the kinetics of the transition on H 3 BO 3 , which could hardly be done by conventional x‐ray diffraction. Raman spectroscopy proved to be a very convenient technique to study the mechanisms behind PIA, owing not only to the fast acquisition of information about phase transition kinetics, but also to the structural information that can be obtained. Copyright © 2003 John Wiley &amp; Sons, Ltd.
γ-Tocotrienol Inhibits Neoplastic Mammary Epithelial Cell Proliferation by Decreasing Akt and Nuclear Factor κB Activity (2005)

Tocotrienols, a subgroup within the vitamin E family of compounds, have been shown to display potent anticancer activity and inhibit preneoplastic and neoplastic mammary epithelial cell proliferation at treatment doses that have little or no effect on normal cell growth and function. However, the specific intracellular mechanisms mediating the antiproliferative effects of tocotrienols are presently unknown. Because Akt and nuclear factor kappaB (NFkappaB) are intimately involved in mammary tumor cell proliferation and survival, studies were conducted to determine the effects of gamma-tocotrienol on Akt and NFkappaB activity in neoplastic +SA mammary epithelial cells in vitro. Treatment with 0-8 microM gamma-tocotrienol for 0-3 days caused a dose-responsive inhibition in +SA cell growth and mitotic activity, as determined by MTT colorimetric assay and proliferating cell nuclear antigen immunocytochemical staining, respectively. Studies also showed that treatment with 4 microM gamma-tocotrienol, a dose that inhibited +SA cell growth by more than 50% compared with that of untreated control cells, decreased intracellular levels of activated phosphotidylinositol 3-kinase-dependent kinase (PI3K)-dependent kinase 1 (phospho-PDK-1) and Akt, and reduced phospho-Akt kinase activity. Furthermore, these effects were not found to be associated with an increase in either phosphatase and tensin homologue deleted from chromosome 10 (PTEN) or protein phosphatase type 2A phosphatase activity. In addition, gamma-tocotrienol treatment was shown to decrease NFkappaB transcriptional activity, apparently by suppressing the activation of IkappaB-kinase-alpha/beta, an enzyme associated with inducing NFkappaB activation. In summary, these findings demonstrate that the antiproliferative effects of gamma-tocotrienol result, at least in part, from a reduction in Akt and NFkappaB activity in neoplastic +SA mammary epithelial cells.
Diffraction of atoms by light: The near-resonant Kapitza-Dirac effect (1986)

We have observed the Kapitza-Dirac effect in the scattering of sodium atoms by a nearly resonant standing-wave laser field. The data clearly show diffraction peaks of the atomic momentum transfer at even multiples of the photon momentum. Theoretical predictions for an off-resonant, adiabatic interaction with a two-state system are in reasonable agreement with the data.
On the PAR Reduction of OFDM Signals UsingMultiple Signal Representation (2004)

Multiple signal representation (MSR) techniques have been used to reduce the high peak-to-average power ratios (PAR) of orthogonal frequency division multiplexing (OFDM) signals. These includes partial transmit sequences (PTS), selected mapping (SLM), selective scrambling and interleaving. All MSR techniques often improve the PAR statistics and are iterative in nature. The PAR reduction obtainable depends on the number of iterations performed, which also increases the complexity of the OFDM transmitter. However, a means to estimate the achievable PAR reduction for a given number of iterations has not been reported in the literature so far. This paper derives a lower bound on the achievable PAR when a MSR technique with a given complexity is used. Our analytical results show a clear asymptotic behavior of the PAR as the number of iterations is increased. Simulation results justify the significance and accuracy of the PAR bound derived.
Release of Cell-free MicroRNA Tumor Biomarkers into the Blood Circulation with Pulsed Focused Ultrasound: A Noninvasive, Anatomically Localized, Molecular Liquid Biopsy (2016)

Purpose To compare the abilities of three pulsed focused ultrasound regimes (that cause tissue liquefaction, permeabilization, or mild heating) to release tumor-derived microRNA into the circulation in vivo and to evaluate release dynamics. Materials and Methods All rat experiments were approved by the University of Washington Institutional Animal Care and Use Committee. Reverse-transcription quantitative polymerase chain reaction array profiling was used to identify candidate microRNA biomarkers in a rat solid tumor cell line. Rats subcutaneously grafted with these cells were randomly assigned among three pulsed focused ultrasound treatment groups: (a) local tissue liquefaction via boiling histotripsy, (b) tissue permeabilization via inertial cavitation, and (c) mild (<10°C) heating of tissue, as well as a sham-treated control group. Blood specimens were drawn immediately prior to treatment and serially over 24 hours afterward. Plasma microRNA was quantified with reverse-transcription quantitative polymerase chain reaction, and statistical significance was determined with one-way analysis of variance (Kruskal-Wallis and Friedman tests), followed by the Dunn multiple-comparisons test. Results After tissue liquefaction and cavitation treatments (but not mild heating), plasma quantities of candidate biomarkers increased significantly (P value range, <.0001 to .04) relative to sham-treated controls. A threefold to 32-fold increase occurred within 15 minutes after initiation of pulsed focused ultrasound tumor treatment, and these increases persisted for 3 hours. Histologic examination confirmed complete liquefaction of the targeted tumor area with boiling histotripsy, in addition to areas of petechial hemorrhage and tissue disruption by means of cavitation-based treatment. Conclusion Mechanical tumor tissue disruption with pulsed focused ultrasound-induced bubble activity significantly increases the plasma abundance of tumor-derived microRNA rapidly after treatment. <sup>©</sup> RSNA, 2016 Online supplemental material is available for this article.
Recent advances in antibacterial drugs (2013)

The incidence of antimicrobial resistance is on continued rise with a threat to return to the "pre-antibiotic" era. This has led to emergence of such bacterial infections which are essentially untreatable by the current armamentarium of available treatment options. Various efforts have been made to develop the newer antimicrobials with novel modes of action which can act against these multi-drug resistant strains. This review aims to focus on these newly available and investigational antibacterials approved after year 2000, their mechanism of actions/resistance, and spectrum of activity and their phases of clinical trials. Newer unexploited targets and strategies for the next generation of antimicrobial drugs for combating the drug resistance and emerging pathogens in the 21(st) century have also been reviewed in the present article.
Bilateral transverse sinus stenosis predicts IIH without papilledema in patients with migraine (2006)

Of patients with migraine, 6.7% had bilateral transverse sinus stenosis; 67.8% of these patients had idiopathic intracranial hypertension without papilledema (IIHWOP). These results suggest that patients with migraine who present bilateral transverse sinus stenosis on cerebral MR venography should undergo lumbar puncture to exclude IIHWOP.
Genomic composition and evolution of Aedes aegyptichromosomes revealed by the analysis of physically mapped supercontigs (2014)

The study developed a physical map of 45% of the Ae. aegypti genome and provided new insights into genomic composition and evolution of Ae. aegypti chromosomes. Our data suggest that minisatellites rather than transposable elements played a major role in rapid evolution of chromosome 1 in the Aedes lineage. The research tools and information generated by this study contribute to a more complete understanding of the genome organization and evolution in mosquitoes.
Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. (1987)

Human placental alkaline phosphatase (PALPase) has been transiently expressed in simian (COS) cells by transfection with a eukaryotic expression vector containing the corresponding cDNA. The level of expression of PALPase was high, and it was produced in an enzymatically active form. The bulk of PALPase was associated with the cell membrane as shown by immunocytochemistry and subcellular fractionation studies. The PALPase produced by transfected COS cells, like PALPase in human tissue, was specifically released from the intact cells in a hydrophilic form by phosphatidylinositol-specific phospholipase C and is, therefore, apparently attached to the outer membrane by means of a phosphatidylinositol-glycan. Transfected COS cells appear to be an excellent model for elucidating the mechanism of attachment of this phosphatidylinositol-glycan to a protein moiety.
Molecular Determinants of Ebola Virus Virulence in Mice (2006)

Zaire ebolavirus (ZEBOV) causes severe hemorrhagic fever in humans and nonhuman primates, with fatality rates in humans of up to 90%. The molecular basis for the extreme virulence of ZEBOV remains elusive. While adult mice resist ZEBOV infection, the Mayinga strain of the virus has been adapted to cause lethal infection in these animals. To understand the pathogenesis underlying the extreme virulence of Ebola virus (EBOV), here we identified the mutations responsible for the acquisition of the high virulence of the adapted Mayinga strain in mice, by using reverse genetics. We found that mutations in viral protein 24 and in the nucleoprotein were primarily responsible for the acquisition of high virulence. Moreover, the role of these proteins in virulence correlated with their ability to evade type I interferon-stimulated antiviral responses. These findings suggest a critical role for overcoming the interferon-induced antiviral state in the pathogenicity of EBOV and offer new insights into the pathogenesis of EBOV infection.
Phosphorylation of Serine 256 by Protein Kinase B Disrupts Transactivation by FKHR and Mediates Effects of Insulin on Insulin-like Growth Factor-binding Protein-1 Promoter Activity through a Conserved Insulin Response Sequence (1999)

Insulin inhibits the expression of multiple genes in the liver containing an insulin response sequence (IRS) (CAAAA(C/T)AA), and we have reported that protein kinase B (PKB) mediates this effect of insulin. Genetic studies in Caenorhabditis elegans indicate that daf-16, a forkhead/winged-helix transcription factor, is a major target of the insulin receptor-PKB signaling pathway. FKHR, a human homologue of daf-16, contains three PKB sites and is expressed in the liver. Reporter gene studies in HepG2 hepatoma cells show that FKHR stimulates insulin-like growth factor-binding protein-1 promoter activity through an IRS, and introduction of IRSs confers this effect on a heterologous promoter. Insulin disrupts IRS-dependent transactivation by FKHR, and phosphorylation of Ser-256 by PKB is necessary and sufficient to mediate this effect. Antisense studies indicate that FKHR contributes to basal promoter function and is required to mediate effects of insulin and PKB on promoter activity via an IRS. To our knowledge, these results provide the first report that FKHR stimulates promoter activity through an IRS and that phosphorylation of FKHR by PKB mediates effects of insulin on gene expression. Signaling to FKHR-related forkhead proteins via PKB may provide an evolutionarily conserved mechanism by which insulin and related factors regulate gene expression.
Quinoxaline studies. XXII. Tosylation and chiralities of 2-substituted 1,2,3,4-tetrahydroquinoxalines (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuinoxaline studies. XXII. Tosylation and chiralities of 2-substituted 1,2,3,4-tetrahydroquinoxalinesGeorge H. Fisher and Harry P. SchultzCite this: J. Org. Chem. 1974, 39, 5, 635–640Publication Date (Print):March 1, 1974Publication History Published online1 May 2002Published inissue 1 March 1974https://doi.org/10.1021/jo00919a012RIGHTS & PERMISSIONSArticle Views267Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (802 KB) Get e-Alerts Get e-Alerts
On the capacity of a pulse position hopped CDMA system (2002)

Pulse position hopping (PPH) is a new promising multiple access technique which has several benefits, such as coherent reception, low transmit power and it can be constructed to be near-far robust. Analysis shows that, it can reach an order of several thousands of active users per cell. In this paper we have estimated the effective capacity for the uplink and the downlink communication in a PPH spread spectrum system.
Transforming Growth Factor-β Induces Secretion of Activated ADAMTS-2 (2003)

The metalloproteinase ADAMTS-2 has procollagen I N-proteinase activity capable of cleaving procollagens I and II N-propeptides in vitro, whereas mutations in the ADAMTS-2 gene in dermatosparaxis and Ehlers-Danlos syndrome VIIC show this enzyme to be responsible in vivo for most biosynthetic processing of procollagen I N-propeptides in skin. Yet despite its important role in the regulation of collagen deposition, information regarding regulation and substrate specificity of ADAMTS-2 has remained sparse. Here we demonstrate that ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-beta 1 (TGF-beta 1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. TGF-beta 1 induced ADAMTS-2 mRNA approximately 8-fold in MG-63 osteosarcoma cells in a dose- and time-dependent, cycloheximide-inhibitable manner, which appeared to operate at the transcriptional level. Secreted ADAMTS-2 protein induced by TGF-beta 1 was 132 kDa and was identical in size to the fully processed, active form of the protease. Biosynthetic processing of ADAMTS-2 to yield the 132-kDa form is shown to be a two-step process involving sequential cleavage by furin-like convertases at two sites. Surprisingly, purified recombinant ADAMTS-2 is shown to cleave procollagen III N-propeptides as effectively as those of procollagens I and II, whereas processing of procollagen III is shown to be decreased in Ehlers-Danlos VIIC. Thus, the dogma that procollagen I and procollagen III N-proteinase activities are provided by separate enzymes appears to be false, whereas the phenotypes of dermatosparaxis and Ehlers-Danlos VIIC may arise from defects in both type I and type III collagen biosynthesis.
A randomised, controlled trial of bosentan in severe COPD (2008)

Pulmonary hypertension during exercise is common in severe chronic obstructive pulmonary disease (COPD). It was hypothesised that the use of the endothelin-receptor antagonist bosentan can improve cardiopulmonary haemodynamics during exercise, thus increasing exercise tolerance in patients with severe COPD. In the present double-blind, placebo-controlled study, 30 patients with severe or very severe COPD were randomly assigned in a 2:1 ratio to receive either bosentan or placebo for 12 weeks. The primary end-point was change in the 6-min walking distance. Secondary end-points included changes in health-related quality of life, lung function, cardiac haemodynamics, maximal oxygen uptake and pulmonary perfusion patterns. Compared with placebo, patients treated with bosentan during 12 weeks showed no significant improvement in exercise capacity as measured by the 6-min walking distance (mean+/-SD 331+/-123 versus 329+/-94 m). There was no change in lung function, pulmonary arterial pressure, maximal oxygen uptake or regional pulmonary perfusion pattern. In contrast, arterial oxygen pressure dropped, the alveolar-arterial gradient increased and quality of life deteriorated significantly in patients assigned bosentan. The oral administration of the endothelin receptor antagonist bosentan not only failed to improve exercise capacity but also deteriorated hypoxaemia and functional status in severe chronic obstructive pulmonary disease patients without severe pulmonary hypertension at rest.
Expression of multiple chemokine genes by a human mast cell leukemia. (1994)

The chemokines are a large group of cytokines that are recognized to be important mediators of inflammation. In this study we show that the human mast cell leukemia line HMC-1 is a source of multiple chemokines, including I-309, monocyte chemoattractant protein 1, macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, RANTES, and interleukin-8. I-309 and MCP-1 transcripts are expressed at low levels in unstimulated HMC-1. However, phorbol ester treatment up-regulates these and other chemokine transcript levels and also up-regulates chemokine protein synthesis and secretion. Induction of chemokine transcripts in HMC-1 requires de novo protein synthesis. We compared the effects of anti-inflammatory glucocorticoids on the expression of chemokine genes in HMC-1 to their effects in activated T-cells. We find that methyl-prednisolone reduces MCP-1 but not other chemokine transcripts in HMC-1, even though there are distinct and more general effects on chemokine transcripts in activated T-cells. These effects are attributed to inhibition of transcription rather than transcript stability. Our results suggest that human mast cells may be a source of multiple chemokines, that glucocorticoids may inhibit the expression of only a subset of these chemokines, and that mast cells and T-cell chemokine expression may occur via distinct regulatory pathways.
Slices of Attention in Asynchronous Video Job Interviews (2019)

The impact of non verbal behaviour in a hiring decision remains an open question. Investigating this question is important, as it could provide a better understanding on how to train candidates for job interviews and make recruiters be aware of influential non verbal behaviour. This research has recently been accelerated due to the development of tools for the automatic analysis of social signals (facial expression detection, speech processing, etc), and the emergence of machine learning methods. However, these studies are still mainly based on hand engineered features, which imposes a limit to the discovery of influential social signals. On the other side, deep learning methods are a promising tool to discover complex patterns without the necessity of feature engineering. In this paper, we focus on studying influential non verbal social signals in asynchronous job video interviews that are discovered by deep learning methods. We use a previously published deep learning system that aims at inferring the hirability of a candidate with regard to a sequence of interview questions. One particularity of this system is the use of attention mechanisms, which aim at identifying the relevant parts of an answer. Thus, information at a fine-grained temporal level could be extracted using global (at the interview level) annotations on hirability. While most of the deep learning systems use attention mechanisms to offer a quick visualization of slices when a rise of attention occurs, we perform an in-depth analysis to understand what happens during these moments. First, we propose a methodology to automatically extract slices where there is a rise of attention (attention slices). Second, we study the content of attention slices by comparing them with randomly sampled slices. Finally, we show that they bear significantly more information for hirability than randomly sampled slices, and that such information is related to visual cues associated with anxiety and turn taking.
Immunomodulatory properties of soluble recombinant human CD58 (LFA-3) molecules. (1992)

The interaction between the E-rosette receptor (CD2) expressed on the cell surface of T lymphocytes and its natural ligand CD58 (LFA-3), a broadly distributed cell surface molecule, represents a potential target for immune modulation. To explore this possibility, we have expressed a soluble recombinant form of human CD58 which exerts potent biological effects in that it acts as a competitive inhibitor for cell surface CD58 in its binding to CD2 positive lymphocytes. Thus, recombinant CD58 blocks NK-mediated cytotoxicity, the mixed lymphocyte reaction, and the T lymphocyte adhesion to CD58 positive cells. In contrast, recombinant CD58 synergized with mitogenic CD2R monoclonal antibodies (anti-T11(2/3) or 9.1) in T cell triggering.