gebhart/patrepeval huggingface dataset:
Progress Toward Skeletal Gene Therapy (2004)

Skeletal gene therapy is an attractive new approach to the treatment of bone disorders. Impressive advances in our knowledge of the molecular genetic basis of skeletal disorders and fracture healing have led to the development of novel therapeutics based on ectopic expression of one or more genes in patient cells that can influence repair or regenerative processes in bone. Although still a relatively immature field, proof-of-principle for enhanced bone formation through skeletal gene therapy has already been established. The challenge now is to more precisely define optimal cellular targets and therapeutic genes, and to develop safe and efficient ways to deliver therapeutic genes to target cells. In this review, we will highlight some of the exciting advances that have been made in skeletal gene therapy in recent years, with a focus on treatment of localized skeletal lesions. Strengths and weaknesses of current approaches will be discussed, as will strategies for improved safety and therapeutic outcome in the future. Skeletal gene therapy can have an enormous impact on patient care. The next 5 years will present us with unparalleled opportunities to develop more effective therapeutic strategies and overcome obstacles presented by current gene transfer technologies.
BatchPrimer3: A high throughput web application for PCR and sequencing primer design (2008)

BatchPrimer3 is a comprehensive web primer design program to develop different types of primers in a high-throughput manner. Additional methods of primer design can be easily integrated into future versions of BatchPrimer3. The program with source code and thousands of PCR and sequencing primers designed for wheat and Brachypodium are accessible at http://wheat.pw.usda.gov/demos/BatchPrimer3/.
Electrochemical Modification of Graphite Felt Electrode for Vanadium Redox Flow Battery (2006)

Electrochemical oxidation of graphite felt was investigated so as to enhance the electrochemical performance of this material for using in vanadium redox cell. Electrochemical activity of the graphite felt in vanadium electrolyte increases with its extent of oxidation. Cell voltage efficiency of over 90% and current efficiency of over 92% are obtained after treatment. Both XPS and FT-IR analysis demonstrates that surface functional groups of COOH increase mainly compared with untreated samples. SEM shows the surfaces are eroded. BET/N-2 measurements show the surface area increases. The improvement of electrochemical activity for the electrode is ascribed to the increase of the number of COOH group and the special surface.
Synthesis of ABC‐Triblock Peptide‐Polymer Conjugates for the Positioning of Peptide Segments within Block Copolymer Aggregates (2007)

Abstract The synthesis of amphiphilic ABC‐triblock peptide‐polymer conjugates comprising a central oligopeptide segment and terminal hydrophilic poly(ethylene oxide) (PEO) as well as hydrophobic poly(butyl acrylate) (PBA) blocks is described. In aqueous solution, the amphiphilic segments of these functional conjugates direct the self‐assembly into block copolymer micelles, while the functional oligopeptide is positioned at the hydrophilic and hydrophobic interface. For that the conjugate PEO‐Arg 10 was synthesized using solid‐phase supported peptide synthesis techniques (SPPS). The supported precursor conjugate was functionalized at the amino‐terminus of the peptide segment with a chain‐transfer moiety for RAFT polymerization. Subsequently, the AB macro‐CTA was liberated from the support and used to polymerize BA homogenously in solution. The RAFT radical polymerization process allowed controlling the molecular weight of the PBA‐block, leading to amphiphilic ABC‐conjugates with low polydispersities. The triblock peptide‐polymer conjugate assembles in water into micellar aggregates, as was shown by light scattering and AFM. The molecular architecture of the ABC‐triblock conjugate controls the positioning of the functional but expensive oligopeptide‐segment within the aggregates, while the inexpensive synthetic polymer blocks are determining the aggregation behavior. Considering the design of the conjugate, the central Arg 10 segment can be expected to be positioned at the interface between the hydrophobic and the hydrophilic polymer blocks, resulting in the formation of a functional domain with precisely controllable functionalities. magnified image
Gradient based fast mode decision algorithm for intra prediction in HEVC (2012)

High Efficiency Video Coding (HEVC) is an ongoing video compression standard and a successor to H.264/AVC. It aims to provide significantly improved compression performance as compared to all existing video coding standards. In the intra prediction of HEVC, this is being achieved by providing up to 35 intra modes with larger coding unit. The optimal mode is selected by a rough mode decision (RMD) process from all of the available modes first, and then through the rate-distortion optimization (RDO) process for the final decision. Because the fact that every coding unit with different sizes is traversed in both procedures makes it very time-consuming, a gradient based fast mode decision algorithm is proposed in this paper to reduce the computational complexity of HEVC. Prior to intra prediction, gradient directions are calculated and a gradient-mode histogram is generated for each coding unit. Based on the distribution of the histogram, only a small part of the candidate modes are chosen for the RMD and the RDO processes. As compared to the default encoding scheme in HEVC test model HM 4.0, experimental results show that the fast intra mode decision scheme provides almost 20% time savings in all intra low complexity cases on average with negligible loss of coding efficiency.
Electrochemical Tattoo Biosensors for Real-Time Noninvasive Lactate Monitoring in Human Perspiration (2013)

The present work describes the first example of real-time noninvasive lactate sensing in human perspiration during exercise events using a flexible printed temporary-transfer tattoo electrochemical biosensor that conforms to the wearer's skin. The new skin-worn enzymatic biosensor exhibits chemical selectivity toward lactate with linearity up to 20 mM and demonstrates resiliency against continuous mechanical deformation expected from epidermal wear. The device was applied successfully to human subjects for real-time continuous monitoring of sweat lactate dynamics during prolonged cycling exercise. The resulting temporal lactate profiles reflect changes in the production of sweat lactate upon varying the exercise intensity. Such skin-worn metabolite biosensors could lead to useful insights into physical performance and overall physiological status, hence offering considerable promise for diverse sport, military, and biomedical applications.
Does the Importance of the C-Terminal Residues in the Maturation of RgpB from Porphyromonas gingivalis Reveal a Novel Mechanism for Protein Export in a Subgroup of Gram-Negative Bacteria? (2006)

The mature 507-residue RgpB protein belongs to an important class of extracellular outer membrane-associated proteases, the gingipains, from the oral pathogen Porphyromonas gingivalis that has been shown to play a central role in the virulence of the organism. The C termini of these gingipains along with other outer membrane proteins from the organism share homologous sequences and have been suggested to function in attachment of these proteins to the outer membrane. In this report, we have created a series of truncated and site-directed mutants of the C terminus from a representative member of this class, the RgpB protease, to investigate its role in the maturation of these proteins. Truncation of the last two residues (valyl-lysine) from the C terminus is sufficient to create an inactive version of the protein that lacks the posttranslational glycosylation seen in the wild type, and the protein remains trapped behind the outer membrane. Alanine scanning of the last five residues revealed the importance of the C-terminal motif in mediating correct posttranslational modification of the protein. This result may have a wider implication in a novel secretory pathway in distinct members of the Cytophaga-Flavobacterium-Bacteroidetes phylum.
1976 and 2009 H1N1 Influenza Virus Vaccines Boost Anti-Hemagglutinin Stalk Antibodies in Humans (2012)

These results demonstrate that vaccines containing viruses whose HA head domains that differ substantially from seasonal strains are capable of boosting titers of HA stalk antibodies. Furthermore, anti-HA stalk antibodies elicited by vaccination appear to be long-lived and therefore could be targeted for the generation of a universal IAV vaccine.
Enzymatic activities associated with a purified simian virus 40 T antigen-related protein (1979)

A protein antigenically related to the simian virus (SV 40) A gene product has been purified to near homogeneity from cells infected with the adenovirus-SV 40 hybrid virus Ad2(+)D2 and shown to contain ATPase (ATP phosphohydrolase, EC 3.6.1.3) and protein kinase (ATP:phosphotransferase, EC 2.7.1.37) activity. Both enzymatic activities copurify with the protein through six stages including one gel filtration column, two ion exchange columns, and a heparin affinity column. Analogous fractions from extracts of cells uninfected or infected with adenovirus 2 alone do not contain these enzymatic activities. The D2 hybrid protein resolves into two forms (I and II) during ion exchange chromatography. Form I, the major species (85%) of the D2 hybrid protein, elutes from DEAE-Sephadex in 0.37 M NaCl and is able to catalyze the hydrolysis of ATP to ADP + P(i) at a rate of 3 mumol/hr per mg. The remaining 10-15% of the D2 hybrid protein consists of form II which elutes from DEAE-Sephadex in 0.29 M NaCl and is able to hydrolyze ATP as well as to incorporate phosphorus from ATP into either the D2 hybrid protein itself or other protein acceptors such as phosvitin. Although both forms are able to bind DNA, the ATPase activity of form I cosediments with SV 40 DNA more efficiently than does the protein kinase activity of form II during glycerol gradient centrifugation. The ATPase activity of form I is efficiently inhibited by addition of anti-T gamma globulin to the reaction mixture whereas control gamma globulin has no effect. Similarly, the phosphorylation of the D2 hybrid protein by form II is inhibited by anti-T gamma globulin. By contrast, phosphorylation of phosvitin is specifically inhibited by antibody only when the immune complex is removed from the reaction mixture. Thus, it appears likely that one and possibly two enzymatic activities are carried out by the D2 hybrid protein. These findings are discussed in terms of mechanisms of SV 40 DNA replication and virally induced transformation.
Selective JAK inhibitors in development for rheumatoid arthritis (2014)

Introduction: The JAK kinases are a family of four tyrosine receptor kinases that play a pivotal role in cytokine receptor signalling pathways via their interaction with signal transducers and activators of transcription proteins. Selective inhibitors of JAK kinases are viewed as of considerable potential as disease-modifying anti-inflammatory drugs for the treatment of rheumatoid arthritis.Areas covered: This article provides a review of the clinical development and available clinical results for those JAK inhibitors currently under investigation. Phase II data for four JAK inhibitors (baricitinib, decernotinib, filgotinib and INCB-039110) are contrasted with that reported for the recently approved JAK inhibitor tofacitinib. The preclinical data on these, in addition to peficitinib, ABT-494, INCB-047986 and AC-410 are also discussed, as are some of the inhibitors in preclinical development.Expert opinion: JAK inhibitors are effective in the treatment of rheumatoid arthritis as evidenced by several inhibitors enabling the majority of treated patients to achieve ACR20 responses, with baricitinib and INCB-039110 both effective when administered once daily. JAK inhibitors differ in isoform specificity profiles, with good efficacy achievable by selective inhibition of either JAK1 (filgotinib or INCB-039110) or JAK3 (decernotinib). It remains to be seen what selectivity provides the optimal side-effect profile and to what extent inhibition of JAK2 should be avoided.
Phospholipid hydroperoxide glutathione peroxidase is the 18-kDa selenoprotein expressed in human tumor cell lines. (1991)

Human tumor cell lines cultured in 75Se-containing media demonstrate four major 75Se-labeled cellular proteins (57, 22, 18, and 12 kDa) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Among these selenoproteins, an enzymatic activity is known only for the 22-kDa protein, since this protein has been identified as the monomer of glutathione peroxidase. However, all tested cell lines also contained a peroxidase activity with phospholipid hydroperoxides that is completely accounted for by the other selenoenzyme, phospholipid hydroperoxide glutathione peroxidase (PHGPX) (Ursini, F., Maiorino, M., and Gregolin, C. (1985) Biochim. Biophys. Acta 839, 62-70). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography of 75Se-labeled proteins separated by gel permeation chromatography supported the identification of PHGPX as the monomeric protein matching the 18 kDa band. This paper is the first report on the identification of PHGPX in human cells.
Inhibition of CCR2 Ameliorates Insulin Resistance and Hepatic Steatosis in db/db Mice (2008)

Propagermanium improved obesity and related metabolic disorders, such as insulin resistance and hepatic steatosis by suppressing inflammation in adipose tissue. Our data indicate that inhibition of CCR2 could improve obesity and type 2 diabetes by interfering adipose tissue inflammation, and that propagermanium may be a beneficial drug for the treatment of the metabolic syndrome.
Isolation of a Herpes Simplex Virus-Specific Antigenic Fraction Which Stimulates the Production of Neutralizing Antibody (1972)

Infection of mammalian cells with herpes simplex virus (HSV) results in the production of a number of virus-induced soluble antigens. Immunodiffusion analyses of the soluble antigen mixture (SAM) obtained from HSV-infected KB or BHK cells revealed at least six well-defined immunoprecipitin bands. Calcium phosphate chromatography (Brushite) was employed to separate one immunoprecipitin (designated CP-1) from the remaining viral and host antigens. We conclude that CP-1 is a viral-specific antigen because (i) specific antiserum, which had been repeatedly absorbed with uninfected cell extracts or serum components, still retained the capacity to react in gel diffusion with CP-1 antigen; (ii) anti-CP-1 serum reacted in gel diffusion with SAM, yielding one precipitin band in identity with the band formed against human gamma globulin; (iii) the CP-1 fraction stimulated the production of HSV-neutralizing antibody of high capacity. The last observation suggests that fraction CP-1 contains a biologically active structural component of the virus which is associated with the envelope. The CP-1 immunoprecipitin was separated from SAM by an alternative method by using a cyanogen bromide-linked immunosorbent prepared from anti-CP-1 gamma globulin. The observation that the CP-1 antigen isolated from the immunosorbent effectively blocked serum-neutralizing activity provided further evidence that neutralizing antibody was directed against CP-1. Acrylamide gel electrophoresis and immunological experiments suggest that the CP-1 antigen is in part a glycoprotein. The finding that CP-1 contains only one antigenic component of the virus will permit future biological studies to be made with a monoprecipitin antiserum. In addition, the techniques described in this paper represent initial steps in the purification of HSV antigens.
Pulsed field gradient NMR study of probe motion in polyacrylamide gels (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPulsed field gradient NMR study of probe motion in polyacrylamide gelsStephen J. Gibbs and Charles S. Johnson Jr.Cite this: Macromolecules 1991, 24, 23, 6110–6113Publication Date (Print):November 1, 1991Publication History Published online1 May 2002Published inissue 1 November 1991https://pubs.acs.org/doi/10.1021/ma00023a010https://doi.org/10.1021/ma00023a010research-articleACS PublicationsRequest reuse permissionsArticle Views217Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Cytosine Analogue Capable of Clamp-Like Binding to a Guanine in Helical Nucleic Acids (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA Cytosine Analogue Capable of Clamp-Like Binding to a Guanine in Helical Nucleic AcidsKuei-Ying Lin and Mark D. MatteucciView Author Information Gilead Sciences, 333 Lakeside Drive Foster City, California 94404 Cite this: J. Am. Chem. Soc. 1998, 120, 33, 8531–8532Publication Date (Web):August 7, 1998Publication History Received16 April 1998Published online7 August 1998Published inissue 1 August 1998https://pubs.acs.org/doi/10.1021/ja981286zhttps://doi.org/10.1021/ja981286zrapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views1500Altmetric-Citations119LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Cytosine,Genetics,Guanine,Noncovalent interactions,Salts Get e-Alerts
Partial Purification and Characterization of Two Non K99 Mannoseresistant Haemagglutinins of Escherichia coli B41 (1983)

A K99-variant of Escherichia coli B41 was produced by growing the parent strain in the presence of antiserum to E. coli K12K99. Two mannose-resistant and eluting (MRE) haemagglutinins with molecular weights greater than 20 x 10(6) were extracted from the cell surface of the variant. One was an anionic antigen, partially purified by ammonium sulphate and isoelectric point precipitation, which adhered to calf intestinal brush borders; it was a protein composed of subunits with mol. wt 34000. Electron microscopy showed that this material did not have a regular fimbrial appearance, but contained some fine fibrillar structures. A second MRE haemagglutinin which was also partially purified by ammonium sulphate precipitation, had a definite fimbrial structure, being a protein composed of two subunits of mol. wt 49500 and 48000. This antigen was probably responsible for the fimbrial appearance of the K99-variant, but it was antigenically distinct from the anionic adhesin and did not adhere to calf intestinal brush borders.
Induction of zebrafish (Brachydanio rerio) P450in vivoand in cell culture (1994)

1. Induction of zebrafish P450 by 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) was studied in liver tissue, primary liver cell culture and multipassage cell culture derived from zebrafish haploid and diploid embryos and liver. 2. TCDD induced two hepatic proteins (54 and 50 kDa) in vivo which were recognized by anti-trout P4501A1 IgG. The 54-kDa protein was induced by TCDD in primary and multipassage hepatocyte cultures and in haploid and diploid embryo-derived cells. The proteins in liver homogenates were not induced by aqueous exposure of zebrafish to beta-naphthoflavone (BNF). 3. Homogenates of zebrafish liver, cultured hepatocytes and embryo-derived cells also exhibited increased ethoxyresorufin O-deethylase (EROD) and 7-12-dimethyl-benz[a]anthracene (DMBA) hydroxylase activity following TCDD exposure.
Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases (2007)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInvestigating and Exploiting the Electrocatalytic Properties of HydrogenasesKylie A. Vincent, Alison Parkin, and Fraser A. ArmstrongView Author Information Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom Cite this: Chem. Rev. 2007, 107, 10, 4366–4413Publication Date (Web):September 11, 2007Publication History Received23 February 2007Published online11 September 2007Published inissue 1 October 2007https://pubs.acs.org/doi/10.1021/cr050191uhttps://doi.org/10.1021/cr050191uresearch-articleACS PublicationsCopyright © 2007 American Chemical SocietyRequest reuse permissionsArticle Views7630Altmetric-Citations669LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Electrodes,Genetics,Oxidation,Peptides and proteins,Redox reactions Get e-Alerts
Die GKSA skakel voltyds met die Bybelgenootskap van Suid-Afrika (BSA) (2004)

Soos reeds kennis gegee in Die Kerkblad van November 2003, is ek voltyds by die Bybelgenootskap aangestel in September 2003. Ds. Gert Hattingh het vir bykans 30 jaar met gemeentes geskakel, en u sal hom goed onthou. Die Sinode het dit goedgedink dat die skakeldeputaat nie 'n predikant hoef te wees nie, en so het die voorreg my te beurt geval om oom Gert se skoene te kom volstaan.
A guide to the recorder (1996)

A guide to the recorder Get access The Cambridge companion to the recorder, ed. John Mansfield Thompson ( Cambridge University Press, 1995), £37.50/$54.95 (hardback), 13.95/$18.95 (paperback) Robert Ehrlich Robert Ehrlich Search for other works by this author on: Oxford Academic Google Scholar Early Music, Volume XXIV, Issue 2, May 1996, Pages 337–338, https://doi.org/10.1093/earlyj/XXIV.2.337 Published: 01 May 1996