gebhart/patrepeval huggingface dataset:
Allergen immunotherapy decreases interleukin 4 production in CD4+ T cells from allergic individuals. (1993)

Allergen specific CD4+ T cell clones generated from allergic individuals have been shown to produce increased levels of the cytokine interleukin 4 (IL-4), compared to allergen specific clones generated from nonallergic individuals. This difference between CD4+ T cells from allergic and nonallergic individuals with regard to cytokine production in response to allergen is thought to be responsible for the development of allergic disease with increased IgE synthesis in atopic individuals. We examined the production of IL-4 in subjects with allergic rhinitis and in allergic individuals treated with allergen immunotherapy, a treatment which involves the subcutaneous administration of increasing doses of allergen and which is highly effective and beneficial for individuals with severe allergic rhinitis. We demonstrated that the quantity of IL-4 produced by allergen specific memory CD4+ T cells from allergic individuals could be considerably reduced by in vivo treatment with allergen (allergen immunotherapy). Immunotherapy reduced IL-4 production by allergen specific CD4+ T cells to levels observed with T cells from nonallergic subjects, or to levels induced with nonallergic antigens such as tetanus toxoid. In most cases the levels of IL-4 produced were inversely related to the length of time on immunotherapy. These observations indicate that immunotherapy accomplishes its clinical effects by reducing IL-4 synthesis in allergen specific CD4+ T cells. In addition, these observations indicate that the cytokine profiles of memory CD4+ T cells can indeed be altered by in vivo therapies. Thus, the cytokine profiles of memory CD4+ T cells are mutable, and are not fixed as had been suggested by studies of murine CD4+ memory T cells. Finally, treatment of allergic diseases with allergen immunotherapy may be a model for other diseases which may require therapies that alter inappropriate cytokine profiles of memory CD4+ T cells.
Theory of long-lived nuclear spin states in solution nuclear magnetic resonance. I. Singlet states in low magnetic field (2005)

We have recently demonstrated the existence of exceptionally long-lived nuclear spin states in solution-state nuclear magnetic resonance. The lifetime of nuclear spin singlet states in systems containing coupled pairs of spins-12 may exceed the conventional relaxation time constant T1 by more than an order of magnitude. These long lifetimes may be observed if the long-lived singlet states are prevented from mixing with rapidly relaxing triplet states. In this paper we provide the detailed theory of an experiment which uses magnetic field cycling to observe slow singlet relaxation. An approximate expression is given for the magnetic field dependence of the singlet relaxation rate constant, using a model of intramolecular dipole-dipole couplings and fluctuating external random fields.
Reductive Electrosynthesis of Crystalline Metal–Organic Frameworks (2011)

Electroreduction of oxoanions affords hydroxide equivalents that induce selective deposition of crystalline metal-organic frameworks (MOFs) on conductive surfaces. The method is illustrated by cathodic electrodeposition of Zn(4)O(BDC)(3) (MOF-5; BDC = 1,4-benzenedicarboxylate), which is deposited at room temperature in only 15 min under cathodic potential. Although many crystalline phases are known in the Zn(2+)/BDC(2-) system, MOF-5 is the only observed crystalline MOF phase under these conditions. This fast and mild method of synthesizing MOFs is amenable to direct surface functionalization and could impact applications requiring conformal coatings of microporous MOFs, such as gas separation membranes and electrochemical sensors.
Evaluation of a Live Attenuated Human Metapneumovirus Vaccine in Adults and Children (2017)

We conducted a phase I clinical trial of an experimental live attenuated recombinant human metapneumovirus (HMPV) vaccine (rHMPV-Pa) sequentially in adults, HMPV-seropositive children, and HMPV-seronegative children, the target population for vaccination. rHMPV-Pa was appropriately restricted in replication in adults and HMPV-seropositive children but was overattenuated for HMPV-seronegative children.
Sodium borohydride reactions under phase-transfer conditions: reduction of azides to amines (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSodium borohydride reactions under phase-transfer conditions: reduction of azides to aminesFranco RollaCite this: J. Org. Chem. 1982, 47, 22, 4327–4329Publication Date (Print):October 1, 1982Publication History Published online1 May 2002Published inissue 1 October 1982https://doi.org/10.1021/jo00143a031RIGHTS & PERMISSIONSArticle Views4688Altmetric-Citations100LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (373 KB) Get e-Alerts Get e-Alerts
Growth and characterization of LNCaP prostate cancer cell spheroids. (1999)

Cells from the prostate tumor cell line LNCaP have been grown as spheroids. The growth kinetics of the spheroids have been characterized by fitting a Gompertz equation to spheroid growth curves. The proliferation state of cells within spheroids of different diameters was assessed by bromodeoxyuridine staining. Scanning and electron transmission microscopy were performed to determine the ultrastructure of the spheroids. Prostate-specific antigen (PSA) secretion was monitored throughout spheroid growth. Consistent with Gompertzian kinetics, the volume of LNCaP spheroids initially increased exponentially and then reached a plateau. The doubling time during the exponential phase was 29 +/- 4 h. A core of nonproliferating cells was seen in spheroids with a diameter of 400 microm; at a diameter of 600 microm, a necrotic core had formed. In smaller, 200-microm diameter spheroids, a core of nonproliferating cells was not seen, but proliferating cells were concentrated at the spheroid periphery. Electron microscopy showed that the spheroids were enveloped by an extracellular matrix and that cell adhesion within the spheroids was due in part to desmosomes. PSA secretion by the spheroids could be modeled as originating from a spherical shell whose thickness was independent of overall spheroid diameter. The shell thickness obtained by fitting an appropriate equation to the data was consistent with that determined from the bromodeoxyuridine studies. LNCaP cells exhibit several important features of prostate cancer cells; in vivo, they are androgen responsive, and they express prostatic acid phosphatase, PSA, and prostate-specific membrane antigen. LNCaP spheroids provide a simple but relevant model for the study of drug delivery and response in prostate cancer.
Body CT : a practical approach (2000)

CT Techniques and Protocols. Lungs. Pleura and Diaphragm. Mediastinum. Cardiovascular CT. Alimentary Tract. Liver and Biliary System. Spleen. Pancreas. Peritoneum and Retroperitoneum. Adrenals. Kidneys. Pelvis. Body Wall. Trauma.
Implants for draining neovascular glaucoma. (1977)

The implant design, surgical technique, and pharmacological methods of controlling bleb fibrosis, used to treat neovascular glaucoma, are described, together with the results of 14 operations performed on 12 eyes.
Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit (2006)

Both extraction systems tested were superior to our standard with regard to DNA yield. This improvement may have a great impact on the success of genotyping in early pregnancy.
Highly Specific and Broadly Potent Inhibitors of Mammalian Secreted Phospholipases A2 (2008)

We report a series of inhibitors of secreted phospholipases A2 (sPLA2s) based on substituted indoles, 6,7-benzoindoles, and indolizines derived from LY315920, a well-known indole-based sPLA2 inhibitor. Using the human group X sPLA2 crystal structure, we prepared a highly potent and selective indole-based inhibitor of this enzyme. Also, we report human and mouse group IIA and IIE specific inhibitors and a substituted 6,7-benzoindole that inhibits nearly all human and mouse sPLA2s in the low nanomolar range.
Isolation of Mesenchymal Stem Cells of Fetal or Maternal Origin from Human Placenta (2004)

Recently we reported that second-trimester amniotic fluid (AF) is an abundant source of fetal mesenchymal stem cells (MSCs). In this study, we analyze the origin of these MSCs and the presence of MSCs in human-term AF. In addition, different parts of the human placenta were studied for the presence of either fetal or maternal MSCs. We compared the phenotype and growth characteristics of MSCs derived from AF and placenta. Cells from human second-trimester (mean gestational age, 19(+2) [standard deviation, +/- 1(+3)] weeks, n = 10) and term third-trimester (mean gestational age, 38(+4) [standard deviation, +/- 1] weeks, n = 10) AF, amnion, decidua basalis, and decidua parietalis were cultured in M199 medium supplemented with 10% fetal calf serum and endothelial cell growth factor. Cultured cells were immunophenotypically characterized, the adipogenic and osteogenic differentiation capacity was tested, and the growth kinetics were analyzed. The origin of fetal and maternal cells was determined by molecular human leukocyte antigen typing. We successfully isolated MSCs from second-trimester AF, amnion, and decidua basalis as well as term amnion, decidua parietalis, and decidua basalis. In contrast, MSCs were cultured from only 2 out of 10 term AF samples. The phenotype of MSCs cultured from different fetal and maternal parts of the placenta was comparable. Maternal MSCs from second-trimester and term decidua basalis and parietalis showed a significantly higher expansion capacity than that of MSCs from adult bone marrow (p < .05). Our results indicate that both fetal and maternal MSCs can be isolated from the human placenta. Amnion is a novel source of fetal MSCs, likely contributing to the presence of MSCs in AF. Decidua basalis and decidua parietalis are sources for maternal MSCs. The expansion potency from both fetal and maternal placenta-derived MSCs was higher compared with adult bone marrow-derived MSCs.
NEUTROPHIL ELASTASE INHIBITOR SUPPRESSES IL-17 BASED INFLAMMATION OF MURINE EXPERIMENTAL COLITIS (2014)

These results suggest that NE inhibitor ameliorated colonic inflammation by decreasing both the activity of NE and the effects of cytokine balance. Clinically, NE inhibitor improves injuries associated with systemic inflammatory response syndrome. Similarly, clinical use of this inhibitor would further clarify its usefulness in clinical colonic inflammation.
<title>Spatially multiplexed composite filters for optical pattern recognition</title> (1993)

A new class of composite filters is proposed. This class of filters can be used in optical correlator based recognition systems. It introduces a novel approach for rotation and scale invariant systems. Also it can be used to improve power throughput without sacrificing the signal-to-ratio in optical correlators based on complex matched filters.
A complex role for complement in allergic asthma (2010)

Allergic asthma is a chronic inflammatory disease of the upper airway. It is well appreciated that maladaptive Th2 immunity promotes the allergic phenotype, the underlying mechanisms of which remain elusive. The disease is associated with activation of complement, an ancient danger-sensing component of the innate immune system. Different models of experimental allergic asthma suggest that the small complement fragments of C3 and C5, the anaphylatoxins C3a and C5a, not only promote proallergic effector functions during the allergic effector phase but regulate the development of Th2 immunity during allergen sensitization. The available data support a concept in which C5a is dominant during allergen sensitization and protects against the development of maladaptive Th2 immunity. By contrast, C3a and C5a appear to act synergistically and drive allergic inflammation during the effector phase. In this article, we will review the recent findings in the field to judge the benefit of complement targeting in allergic asthma.
Head motion measurement and correction usingFIDnavigators (2018)

Quantitative rigid-body motion information can be effectively estimated using the proposed FIDnav-based approach, which represents a practical method for retrospective motion compensation in less cooperative patient populations.
Electron migration in BaFCl:Eu2+ phosphors (1997)

Here we report the electron migration by photo- or thermostimulation in BaFCl:Eu2+. Electrons released from F centers may be trapped by other defect sites to form F aggregates or another type of F center and vice versa. This migration reduces the photostimulated luminescence efficiency, lowers the imaging plate sensitivity, and causes the difference between the optical absorption and photostimulation spectra of color centers.
New Drug Targets for Genomic Cancer Therapy Successes, Limitations, Opportunities and Future Challenges (2001)

Cancer drug therapy is undergoing a major transition from the previous pregenomic cytotoxic era to the new postgenomic era. Future mechanism-based therapeutic agents will increasingly be designed to act on molecular targets that are causally involved in the malignant progression of human cancers. Such agents are predicted to show greater therapeutic selectivity for cancer versus normal cells. New cancer drug targets are identified and validated in various ways. The determination of the normal human genome sequence, followed by that of multiple cancer genomes, is accelerating target discovery. Other new technologies, particularly high throughput screening, combinatorial chemistry and gene expression microarrays, are increasing the speed and efficiency of drug development. Examples of new molecular therapeutics showing promising activity in the clinic include Herceptin, Glivec and Iressa. However, many challenges remain as we test the vision of individualised combinatorial genome-based therapy, using drugs targeted to every significant molecular abnormality in cancer.
Broadband communications via high-altitude platforms: A survey (2005)

This article is a survey on communication aspects of High Altitude Platforms (HAPs), namely airships or aircraft positioned in the stratosphere between 17 and 22 km. HAPs can be considered as a novel solution for providing telecommunications services. This survey begins with an introduction to HAPs, that is, some historical information and advantages of HAPs compared to terrestrial and satellite networks, followed by information about suitable airships and aircraft, frequency bands allocated to HAPs, possible architectures, and some points on the system structure. We continue with the studies that have been carried out on channel modeling and interference, antennas, transmission and coding techniques. We also refer to access and resource allocation techniques that have been performed so far. Finally, the survey concludes with the types of applications that HAPs are suitable for, in addition to some related projects.
Engineered selection rules for tunable coupling in a superconducting quantum circuit (2009)

Engineering selection rules of transitions is a useful strategy for coupling and decoupling qubits in superconducting quantum circuits. Following this approach, we implement a tunable coupling scheme between two flux qubits. The qubits are coupled parametrically under microwave driving via the nonlinear inductance of a third qubit. The measured on-state coupling as well as the off-state residual coupling depends on the coupler bias and agrees quantitatively with a calculation of transition matrix elements of a three-qubit Hamiltonian.
Reactive oxygen species‐dependent necroptosis in Jurkat T cells induced by pathogenic free‐living Naegleria fowleri (2011)

Naegleria fowleri, a free-living amoeba, is the causative pathogen of primary amoebic meningoencephalitis in humans and experimental mice. N. fowleri is capable of destroying tissues and host cells through lytic necrosis. However, the mechanism by which N. fowleri induces host cell death is unknown. Electron microscopy indicated that incubation of Jurkat T cells with N. fowleri trophozoites induced necrotic morphology of the Jurkat T cells. N. fowleri also induced cytoskeletal protein cleavage, extensive poly (ADP-ribose) polymerase hydrolysis and lactate dehydrogenase (LDH) release. Although no activation of caspase-3 was observed in Jurkat T cells co-incubated with amoebae, intracellular reactive oxygen species (ROS) were strongly generated by NADPH oxidase (NOX). Pretreating cells with necroptosis inhibitor necrostatin-1 or NOX inhibitor diphenyleneiodonium chloride (DPI) strongly inhibited amoeba-induced ROS generation and Jurkat cell death, whereas pan-caspase inhibitor z-VAD-fmk did not. N. fowleri-derived secretory products (NfSP) strongly induced intracellular ROS generation and cell death. Necroptotic effects of NfSP were effectively inhibited by pretreating NfSP with proteinase K. Moreover, NfSP-induced LDH release and intracellular ROS accumulation were inhibited by pretreating Jurkat T cells with DPI or necrostatin-1. These results suggest that N. fowleri induces ROS-dependent necroptosis in Jurkat T cells.
Construction of Retroviral Packaging Cell Lines (2003)

Most of the time, retrovirus vectors retain only cis-acting sequences from the original viral genome. These sequences allow the recombinant structure to be transcribed (LTR promoter/enhancer) and the RNA to be processed (splicing and polyadenylation signals), packaged into a virion particle (packaging sequences), and replicated by the reverse transcriptase (tRNA binding site, R region, and polypurine track). The other viral functions have to be provided in trans for the assembly of recombinant viral particles to take place. This can be simply achieved by using a replication-competent helper virus, leading to the production of a mixed population. Nevertheless, helper-free stocks are desirable for most applications, since 1. The high frequency of recombination in a mixed virus stock is likely to lead to the appearance of recombinants with unknown structure and activity. These new chimeras, either spread by the helper virus or replication-competent themselves, create a potential safety problem. 2. Cell lineage analysis using retroviral marking can only be performed and interpreted in a helper-free context 3. In vivo gene transfer experiments can be jeopardized by disease (s) associated with helper-virus infection (1).
GENETIC AND CELLULAR THERAPIES (2005)

This chapter discusses several types of genetic and cellular therapies. The factor VIII gene provides many challenges in producing a drug by recombinant DNA (rDNA) means. It is large (26 exons) with a genomic structure extending over 186kb. The protein comprises 2332 amino acids and is synthesized as a single chain that is processed, as it moves from the rough endoplasmic reticulum to the Golgi apparatus. As this occurs, the mid-portion (B subunit) of the molecule is excised since it is not required for hemostatic function. Somatic cell gene therapy can be defined as the transfer of genetic material (DNA or RNA) into the cells of an organism to treat diseases or for marking studies. Gene therapy for cancer can be considered in a number of categories such as to stimulate the natural immunity to cancer cells, to kill or interfere with the growth of cancer cells with drugs, to insert a wild-type tumor suppressor gene, and to increase tolerance to high doses of chemotherapy or delay drug resistance. Apart from a substantial increase in the number of protocols for gene therapy, another significant development has been the change in emphasis from genetic disease to cancer and HIV. Future developments in gene therapy are likely to involve more in vivo strategies for gene transfer.
Semantic Segmentation of Building Roof in Dense Urban Environment with Deep Convolutional Neural Network: A Case Study Using GF2 VHR Imagery in China (2019)

This paper presents a novel approach for semantic segmentation of building roofs in dense urban environments with a Deep Convolution Neural Network (DCNN) using Chinese Very High Resolution (VHR) satellite (i.e., GF2) imagery. To provide an operational end-to-end approach for accurately mapping build roofs with feature extraction and image segmentation, a fully convolutional DCNN with both convolutional and deconvolutional layers is designed to perform building roof segmentation. We selected typical cities with dense and diverse urban environments in different metropolitan regions of China as study areas, and sample images were collected over cities. High performance GPU-mounted workstations are employed to perform the model training and optimization. With the building roof samples collected over different cities, the predictive model with convolution layers is developed for building roof segmentation. The validation shows that the overall accuracy (OA) and the mean Intersection Over Union (mIOU) of DCNN-based semantic segmentation results are 94.67% and 0.85, respectively, and the CRF-refined segmentation results achieved OA of 94.69% and mIOU of 0.83. The results suggest that the proposed approach is a promising solution for building roof mapping with VHR images over large areas in dense urban environments with different building patterns. With the operational acquisition of GF2 VHR imagery, it is expected to develop an automated pipeline of operational built-up area monitoring, and the timely update of building roof map could be applied in urban management and assessment of human settlement-related sustainable development goals over large areas.