gebhart/patrepeval huggingface dataset:
Design and evaluation of a ZP3 peptide vaccine in a homologous primate model (1999)

The concept of a safe, immunocontraceptive vaccine using the zona pellucida glycoprotein 3 (ZP3) as an immunogen has been marred by the appearance of ovarian dysfunction in several species. However, careful selection of epitopes on mouse ZP3 have demonstrated that it is possible to segregate contraceptive bone marrow-derived (B)-cell epitopes from the cytotoxic thymus-derived (T)-cell epitopes thought to be responsible for inducing ovarian disease. B-cell epitopes on marmoset ZP3 (mstZP3) were identified by epitope mapping studies. Using a panel of polyclonal antibodies against recombinant mstZP3, an immunodominant epitope mstZP3(301-320) was identified. A chimeric peptide was co-linearly synthesized incorporating this sequence with a promiscuous tetanus toxoid T-helper cell epitope. Using the common marmoset (Callithrix jacchus) as an animal model, we have compared the consequences of active immunization with homologous recombinant mstZP3 and mstZP3(301-320) chimeric peptide vaccine. Long-term infertility was achieved using mstZP3 but at the expense of ovarian function. In contrast, no disruption to ovarian function was observed following mstZP3(301-320) immunization. Antibodies to this peptide immunolocalized to the zona pellucida of both marmoset and human ovarian sections and inhibited human sperm-zona binding by approximately 60% in vitro. However, in-vivo studies indicated that targeting a single ZP3 epitope was insufficient to reliably and consistently achieve a contraceptive effect.
Redefining Flexible Delivery in Australian Hospitality Degree Programs - the Perceptions of Students (2006)

Flexible delivery was designed to improve student academic performance by designing the students' university experience according to their needs and wants. Many institutions, including universities, have adopted elements of flexible delivery and labelled them as flexible delivery. A review of hospitality degrees at Australian universities and literature analysing student experiences of flexible delivery has not identified or specifically addressed the needs and wants of the hospitality student. This paper represents a small part of a study into the needs and wants of those students studying for a hospitality degree at two Australian universities. One with one university engaging the concept of flexible delivery and the other continuing to operate in the traditional manner, facilitating a comparison of the needs and wants of these two groups. Thus far, this study has identified that the students perceive flexible delivery to mean flexible organisational arrangements and flexible teaching and learning, as they relate to the timing the courses and learning experiences (>80% of responses). Stratification according to program has revealed that there is no significant difference between the two groups of students. Furthermore it has also revealed that the students perceived flexible delivery to be less about the elements of flexible assessment, flexible entry and exit from the program and flexible course arrangement (<20% of responses).
Exploring fatty alcohol-producing capability of Yarrowia lipolytica (2016)

This work preliminarily explored fatty alcohol-producing capability through mobilization of FAR and fatty acid metabolism, maximizing the intracellular fatty alcohol-producing capability, suggesting that Y. lipolytica cell factory potentially offers a promising platform for fatty alcohol production.
Nimrod/G: an architecture for a resource management and scheduling system in a global computational grid (2000)

The availability of powerful microprocessors and high-speed networks as commodity components has enabled high-performance computing on distributed systems (wide-area cluster computing). In this environment, as the resources are usually distributed geographically at various levels (department, enterprise or worldwide), there is a great challenge in integrating, coordinating and presenting them as a single resource to the user, thus forming a computational grid. Another challenge comes from the distributed ownership of resources, with each resource having its own access policy, cost and mechanism. The proposed Nimrod/G grid-enabled resource management and scheduling system builds on our earlier work on Nimrod (D. Abramson et al., 1994, 1995, 1997, 2000) and follows a modular and component-based architecture enabling extensibility, portability, ease of development, and interoperability of independently developed components. It uses the GUSTO (GlobUS TOolkit) services and can be easily extended to operate with any other emerging grid middleware services. It focuses on the management and scheduling of computations over dynamic resources scattered geographically across the Internet at department, enterprise or global levels, with particular emphasis on developing scheduling schemes based on the concept of computational economy for a real testbed, namely the Globus testbed (GUSTO).
Influence of autoregulation and capacitance on diastolic coronary artery pressure-flow relationships in the dog. (1982)

We studied the influence of initial coronary artery pressure on pressure-flow relationships during long diastoles in 22 closed-chest, anesthetized dogs under basal conditions and after maximum coronary vasodilation.The circumflex artery was perfused from a pressurized arterial reservoir through a cannula inserted in the carotid artery.Diastolic pressure-flow relations were obtained under two conditions: (1) when pressure was gradually decreasing (40 mm Hg/sec); and(2) when pressure and flow were constant beginning 500 msec after a rapid step decrease in pressure.Pressure-flow curves obtained with pressure gradually decreasing were linear in the basal state and during maximum coronary vasodilation [r = 0.98 ± 0.01 (SE)].In autoregulating animals, 25 mm Hg increments in coronary artery pressure from 75 to 125 mm Hg resulted in progressive decreases in the slope (2.2 ± 0.1 to 1.2 ± 0.1 ml/min per 100 g per mm Hg, P < 0.01) and increases in the zero flow pressure intercept (37.3 ± 1.4 to 51.2 ± 2.1 mm Hg, P < 0.01) of the pressure-flow curves.These changes were not observed when autoregulation was abolished with intracoronary adenosine.Diastolic pressure-flow curves obtained under constant pressure conditions were also linear in the basal state and during maximum coronary vasodilation (r = 0.98 ± 0.01).Increasing initial coronary artery pressure from 75 to 125 mm Hg resulted in a decrease in the slope (1.6 ± 0.1 to 0.9 ± 0.0 ml/ min per 100 g per mm Hg, P < 0.01) and an increase in the zero flow pressure intercept (21.1 ± 2.1 to 37.2 ± 2.3 mm Hg, P < 0.01) of the pressure-flow curves.With maximum coronary vasodilation, the slope increased to 4.7 ± 0.1 ml/min per 100 g per mm Hg (P < 0.001) and the zero flow pressure intercept decreased to 15.2 ± 0.7 mm Hg (P < 0.001).When pressure-flow curves obtained with pressure gradually decreasing were compared to ones obtained during constant pressure perfusion, the constant pressure values for slope averaged 10-25% lower (P < 0.01), whereas values for the zero flow pressure intercept were 8-12 mm Hg lower (P < 0.01).These data indicate that diastolic coronary artery pressure-flow relations obtained when pressure is continuously changing are influenced by coronary vascular capacitance.When capacitance effects were minimized, the slope and zero flow pressure intercept of the diastolic pressure-flow curves were dependent on coronary vasomotor tone, with the zero flow pressure intercept ranging from 15 to 37 mm Hg.These data are consistent with a vascular waterfall model of diastolic flow regulation.Based on such a model, we estimate that changes in coronary vascular resistance and zero flow pressure intercept can account for 70-80% and 20-30%, respectively, of the coronary autoregulation observed over the pressure range of 75 to 125 mm Hg.
Comparison of clofibrate with halofenate in diabetics with hyperlipidaemia. (1977)

In a double-blind, randomized study which lasted 48 weeks the effects of clofibrate and halofenate were compared in maturity-onset diabetics with hyperlipidaemia. With the use of both clofibrate and halofenate serum cholesterol values were lowered only slightly. Both agents significantly reduced triglyceride values, but the decreases were modest and transient. Both drugs significantly lowered serum urate values, although the effect of halofenate was distinctly greater. Halofenate, but not clofibrate, had a considerable hypoglycaemic effect on the patients, most of whom were also receiving oral antidiabetic medicines. The drugs produced a number of clinical and biochemical adverse reactions, and in about 20% of all patients the trial had to be discontinued prematurely. The management of hyperlipidaemia in maturity-onset diabetics is briefly discussed, and it is concluded that neither clofibrate nor halofenate is to be recommended.
Pathology of Human Influenza A (H5N1) Virus Infection in Cynomolgus Macaques (Macaca fascicularis) (2003)

Infection with influenza A (H5N1) virus, which has not been associated with respiratory disease in humans previously, caused clinical signs of acute respiratory distress syndrome and multiple-organ dysfunction syndrome with high mortality in humans in Hong Kong in 1997. To study the pathogenesis of this disease, we infected four cynomolgus monkeys (Macaca fascicularis) with 2.5 x 104 median tissue culture infectious dose (TCID50) of influenza virus A/Hong Kong/156/97 (H5N1) and euthanatized them 4 or 7 days after infection. The main lesion was a necrotizing broncho-interstitial pneumonia (4/4) similar to those found in primary influenza virus pneumonia in humans, with desquamation of respiratory epithelium (4/4), intra-alveolar hemorrhage (4/4), hyaline membrane formation (2/4), and infiltration with neutrophils and macrophages (4/4). Lesions in other organs consisted of a suppurative tonsillitis (2/4) and necrosis in lymphoid organs (1/4), kidney (1/4), and liver (1/4). By immunohistochemistry, influenza virus antigen was limited to pulmonary tissue (4/4) and tonsils (2/4). Based on these results, we suggest that the cynomolgus monkey is a suitable animal model for studying the pathogenesis of human H5N1 virus infection and that multiple-organ dysfunction syndrome in this disease may be caused by diffuse alveolar damage from virus replication in the lungs alone.
Determination of Toxic Pathways of Metabolism by Deuterium Substitution (1984)

(1984). Determination of Toxic Pathways of Metabolism by Deuterium Substitution. Drug Metabolism Reviews: Vol. 15, No. 7, pp. 1335-1351.
Zidovudine treatment for anti-HIV positive haemophiliacs (2008)

Twenty haemophiliacs (17 CDC group IV and 3 CDC group II) were treated with zidovudine for a median of 37 weeks (range 10-66). Eight (40%) tolerated zidovudine without a dose change. Two patients died and five patients (29%) developed opportunist infections. Haematological toxicity occurred in ten CDC IV patients (59%) but only one case of sepsis occurred in 101 episodes of documented granulocytopenia. Thrombocytopenia responded to treatment with zidovudine in four of five patients. It is concluded that zidovudine is beneficial for symptomatic haemophiliacs and although the haematological toxicity is high, it is mostly asymptomatic, reversible and well tolerated. Two of the three CDC II patients treated with zidovudine progressed to CDC IV, but had low initial T4 lymphocyte counts and were P24 antigen positive.
High signal to noise ratio in low field NMR on chip, simulations and experimental results (2004)

A /spl mu/fluidic chip with integrated microcoil was developed for nuclear magnetic resonance (NMR) spectroscopy on limited volume samples (nanoliters range). This chip will be used for the online monitoring of micro chemical reactions. Design rules for the microcoil to achieve the best signal to noise ratio (SNR) were obtained by finite element simulation. For pure water in a low field NMR magnet (60 MHz., i.e. 1.4 Tesla) a SNR of 550 was achieved which is to our knowledge higher than any other /spl mu/fluidic chip with integrated detection coil. It is also shown that a spectral resolution of 0.025 ppm is obtained when a microchannel with an arbitrary cross section is placed parallel to the magnetic field. A high SNR at low magnetic field for small volume samples is essential for the development of portable NMR systems where mini permanent magnets of 1 Tesla are used.
A recombinant vaccinia virus encoding inducible nitric oxide synthase is attenuated in vivo (1996)

To investigate the role of nitric oxide during vaccinia virus (VV) infection of mice, a recombinant VV encoding the inducible nitric oxide synthase (iNOS) gene (VV-HA-iNOS) was constructed. Following infection of immunocompromised or immunocompetent mice, the virus was highly attenuated compared with a control recombinant VV. Athymic and sublethally irradiated mice survived infection with 10(7) PFU of VV-HA-iNOS, a dose that resulted in uniform mortality in mice infected with the control recombinant VV. Attenuated virus growth was evident as early as 24 h following infection, suggesting that NO had direct antiviral activity. We have previously shown that treatment of mice with the inhibitor of NO production N(G)-methyl-L-arginine did not influence the course of VV infection in mice. The present study has indicated that NO can potentially exert an antiviral effect during murine VV infection. We propose that during VV infection, nitric oxide production contributes to the control of virus growth, but that in its absence, other antiviral mechanisms are sufficient to mediate fully effective virus clearance.
Low-band-gap, sublimable rhenium(I) diimine complex for efficient bulk heterojunction photovoltaic devices (2004)

The use of chlorotricarbonyl rhenium(I) diimine complex as photosensitizing molecule in photovoltaic cells is demonstrated. Unlike other transition-metal-based sensitizers, the complex is sublimable and has low band gap. It also exhibits bipolar charge transport character with relatively high carrier mobilities on the order of 10−3 cm2 V−1 s−1. Multilayer heterojunction and bulk heterojunction devices with fullerene as the electron accepting molecule were prepared. For the bulk heterojunction devices, the fill factor and power conversion efficiency under simulated solar light illumination were 0.56 and 0.48%, respectively. Atomic force microscopic images showed that the complex dispersed evenly with fullerene molecules in solid state.
Charge derivatization of peptides for analysis by mass spectrometry (1998)

The analysis of peptide derivatives by fast atom bombardment, liquid secondary-ionization mass spectrometry, plasma desorption, electrospray ionization, and matrix-assisted laser desorption/ionization is reviewed. The fragmentation patterns of peptides and of charge-derivatized peptides are compared, and the proposed fragment ion structures are summarized. A variety of derivatization approaches and the distinguishing features of mass spectra produced from these derivatives are described. The most promising derivatization approaches are evaluated, and the strengths and limitations of these approaches are discussed. © 1999 John Wiley & Sons, Inc. Mass Spec Rev 17: 255–274, 1998
Non‐iron metalloporphyrins: potent antibacterial compounds that exploit haem/Hb uptake systems of pathogenic bacteria (1999)

A new group of potent antibacterial compounds, non-iron metalloporphyrins (MPs), is described. MPs possess a strong antibacterial activity against Gram-positive bacteria, Gram-negative bacteria and mycobacteria. Anaerobically grown bacteria and microorganisms that do not respire and/or express haem uptake systems were resistant to MPs. Antibacterial activity of MPs was not affected by known antibiotic resistance mechanisms operating in bacteria. The most potent MP against Y. enterocolitica, methicillin-resistant S. aureus and M. smegmatis was gallium protoporphyrin IX (Ga-PPIX). When tested alone, Ga ions and metal-free porphyrins had approximately 100-fold higher minimum inhibitory concentration (MIC) values for these organisms. Ga-PPIX was not degraded by MP-sensitive bacteria, indicating that the whole molecule is responsible for antibacterial activity. MPs are antibacterial 'Trojan horses', as they exploit haem transport systems of Gram-negative bacteria as portals of entry into the cell. Bacterial mutants in superoxide dismutases, catalases and stationary-phase sigma factors were hypersensitive to Ga-PPIX. The extreme sensitivity of sod mutants to MPs and the requirement for active respiration for MP activity suggests that these compounds stimulate the production of reactive oxygen radicals in bacteria. Ga-PPIX was not toxic to primary human fibroblasts, several established cell lines and experimental animals at concentrations > 100-fold higher than the MIC for sensitive bacteria.
Effects of prepartum supplementary fat and muscle hypertrophy genotype on cold tolerance in newborn calves. (1999)

Effects of feeding pregnant dams supplemental dietary fat during the last 55 d of gestation on cold tolerance of newborn crossbred calves with (Piedmontese cross, P, n = 15) or without (Hereford cross, H, n = 16) the muscle hypertrophy allele was determined. Primiparous F1 dams gestating F2 calves of the respective breeds were assigned randomly within breed to receive gestation diets containing either 2.2 (Low Fat; LF) or 5.1% fat (High Fat; HF). Safflower (Carthamus tinctorius L.) seeds containing 37% oil with 79% linoleic acid were the supplemental fat source in diets formulated to be isocaloric-isonitrogenous. At parturition, calves were separated from their dams, fed 38 degrees C pooled dairy cow colostrum (30 mL/kg BW), muzzled to prevent suckling, and returned to their dams in a heated (22 degrees C) room for 3.5 h. At 4 h of age (birth = 0 h), a catheter was inserted into the jugular vein. At 5 h of age, calves were placed in a 0 degrees C room for 140 min, and rectal temperatures and blood samples were obtained at 10- and 20-min intervals. Blood was assayed for cortisol and glucose. Rectal temperature was affected by diet (P<.05), time, diet x time, and breed x time (P<.01 for time and the interactions). Cortisol and glucose concentrations were not affected by diet, breed, or the diet x breed interaction, but they were affected by time, breed x time (both P<.01), and diet x time (P = .06). Calves from HF dams had higher rectal temperatures than calves from LF dams, and the HF calves maintained higher rectal temperatures throughout cold exposure. Cortisol concentrations were lower (P = .06) in calves from HF dams, and these calves had more (P = .06) glucose available for metabolic heat production than calves from LF dams. Piedmontese-cross calves maintained higher (P<.01) rectal temperatures and had higher cortisol and glucose (both P<.01) concentrations than did H-cross calves. We conclude that feeding dams supplemental fat during late gestation increased heat production in newborn calves and potentially could increase calf survival; calves with muscle hypertrophy may have a different ratio of shivering vs nonshivering thermogenesis due to differences in body composition or relationships among uncoupling proteins.
Open computing guide to Mosaic (1995)

Part 1 The basics: introduction to the Internet overview of information process browsing through Mosaic. Part 2 Mosaic client: working playing customization. Part 3 World Wide Web server: configuration creating the information provider server management. Part 4 Installation and troubleshooting: a handy client a secure server. Appendices: list of information providers configuring the client home page.
HEC Binds to the Seventh Regulatory Subunit of the 26 S Proteasome and Modulates the Proteolysis of Mitotic Cyclins (1997)

A newly identified nuclear protein rich in leucine heptad repeats called HEC is important for mitosis. To elucidate its mechanism of action, the region containing leucine heptad repeats was used to identify cellular proteins that potentially interact with HEC. Complementary DNAs encoding several proteins including MSS1, p45, Nek2, and Smc1/Smc2, known to be important for G2/M progression, were identified. The interaction between HEC and MSS1, the seventh regulatory subunit of the 26 S proteasome, was further demonstrated by in vitro GST pull-down assays. HEC is not a part of the 26 S proteasome and interacts with MSS1 only when it is dissociated from the complex during M phase. Purified MSS1 specifically hydrolyzes ATP, an activity inhibited by HEC. In addition, HEC inhibits the proteolysis of mitotic cyclin B in vitro. Consistent with this biochemical activity, ectopic expression of HEC inhibits the degradation of mitotic cyclins after telophase, resulting eventually in cell death. These results show that HEC is a negative regulator of MSS1 and suggest that it may modulate M phase progression, in part, through the regulation of proteasome-mediated degradation of cell cycle regulatory proteins.
Pay 1990: the spectre of stagflation (1989)

As the economy moves into 1990, the outlook is precariously balanced between the risks of recession and inflation, with trends in pay settlements the key to future developments. There is a clear worry that if pay increases are not contained, as demand and and productivity fall off during 1990, pressures on prices will not moderate rapidly. Companies, already faced with a burgeoning financial deficit and a squeeze on profits and liquidity, may then be forced into a period of intense labour shedding, thus tipping the economy towards recession. The intensity of pressures in the labour market have once again raised the spectre of stagflation, albeit of a milder form than previously experienced and in the context of a far more benign international environment; slugflation may be a more appropriate term.
The Myostatin Propeptide and the Follistatin-related Gene Are Inhibitory Binding Proteins of Myostatin in Normal Serum (2002)

Myostatin, also known as growth and differentiation factor 8, is a member of the transforming growth factor beta superfamily that negatively regulates skeletal muscle mass (1). Recent experiments have shown that myostatin activity is detected in serum by a reporter gene assay only after activation by acid, suggesting that native myostatin circulates as a latent complex (2). We have used a monoclonal myostatin antibody, JA16, to isolate the native myostatin complex from normal mouse and human serum. Analysis by mass spectrometry and Western blot shows that circulating myostatin is bound to at least two major proteins, the myostatin propeptide and the follistatin-related gene (FLRG). The myostatin propeptide is known to bind and inhibit myostatin in vitro (3). Here we show that this interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. Studies with recombinant V5-His-tagged FLRG protein confirm a direct interaction between mature myostatin and FLRG. Functional studies show that FLRG inhibits myostatin activity in a reporter gene assay. These experiments suggest that the myostatin propeptide and FLRG are major negative regulators of myostatin in vivo.