gebhart/patrepeval huggingface dataset:
Probiotics and human health: a clinical perspective (2004)

There is unequivocal evidence that administration of probiotics could be effective in the treatment of acute infectious diarrhoea in children and the prevention of antibiotic associated diarrhoea and nosocomial/community acquired diarrhoea. Encouraging evidence is also emerging for the effectiveness of probiotics in the prevention and management of pouchitis and paediatric atopic diseases, and the prevention of postoperative infections. There is also strong evidence that certain probiotic strains are able to enhance immune function, especially in subjects with less than adequate immune function such as the elderly. Efficacy of probiotics in the prevention of traveller's diarrhoea, sepsis associated with severe acute pancreatitis, and cancers, the management of ulcerative colitis, and lowering of blood cholesterol remains unproven. In addition to firm evidence of efficacy (for a range of conditions), major gaps exist in our knowledge regarding the mechanisms by which probiotics modulate various physiological functions and the optimum dose, frequency, and duration of treatment for different probiotic strains.
Decreased cyclic AMP in the epidermis of lesions of psoriasis (1972)

Previously we suggested that incomplete differentiation, accelerated proliferation and glycogen accumulation in epidermis of psoriatic lesions might be due to deficient adenosine 3',5'-monophosphate (cyclic AMP) in epidermis of the lesion. Therefore, we measured endogenous cyclic AMP in involved and uninvolved epidermis of 25 psoriasis patients and normal epidermis of 25 control subjects. A statistically significant decrease in cyclic AMP was found in involved psoriatic epidermis when compared with uninvolved psoriatic and control epidermis. Because cyclic AMP regulates glycogen metabolism and promotes differentiation of cancer cells in culture while limiting their proliferation, the cyclic AMP deficiency may play a pathophysiological role in the rapid epidermal cell cycle kinetics of psoriasis. Therefore, topical application of cyclic AMP analogues or agents which promote cyclic AMP synthesis or retard its hydrolysis might be used therapeutically to either normalize psoriatic differentiation or prevent psoriasis from occurring.
Record Linkage: Statistical Models for Matching Computer Records (1990)

"We wish to measure the evidence that a pair of records relates to the same, rather than different, individuals. The paper emphasizes statistical models which can be fitted to a file of record pairs known to be correctly matched, and then used to estimate likelihood ratios. A number of models are developed and applied to U.K. immigration statistics. The combination of likelihood ratios for possibly correlated record fields is discussed." A series of comments on the paper is also included, as well as a reply to those comments by the author (pp. 312-20).
Overexpression of p16INK4A in liquid‐based specimens (SurePath™) as marker of cervical dysplasia and neoplasia (2002)

Human papillomavirus (HPV) is recognized as a causal agent for cervical carcinomas. Assimilation of HPV oncogenes E6 and E7 into the host DNA promotes upregulation of cyclin dependent kinase inhibitor (CDKI) p16(INK4A), detectable by monoclonal antibody in the developing cervical cancer cells. The aim of this study was to 1) develop a protocol for p16(INK4A) immunocytochemical staining on SurePath preparations, and 2) determine its utility as an HPV marker on a spectrum of cervical reactive and neoplastic lesions. Seventy-two specimens consisting of 28 nonneoplastic/nondysplastic cases (NN), one reactive glandular cells (RGC), 27 low-grade squamous intraepithelial lesions (LSIL), 10 high-grade squamous intraepithelial lesions (HSIL), one squamous cell carcinoma (SCCA), four atypical glandular cells (AGUS), and two adenocarcinomas (ADCA) were reprepped by SurePath and antibody to p16(INK4A) applied at 1:100 dilution using the Dako Envision + System on the Dako Autostainer. Expression of p16(INK4A) within the nucleus principally and cytoplasm of at least 10-15 cells was considered positive. All initial Papanicolaou-stained discrepant cases (p16(INK4A) positivity of NN and RGC cases and lack of reactivity in LSIL, HSIL, and AGUS) were reviewed. Nine of ten (90%) HSIL, one (100%) SCCA, 21/27 (78%) LSIL, and some reactive and inflammatory specimens demonstrated the presence of p16(INK4A). Reevaluation of discrepant cases revealed that several were underinterpreted (four NN were LSIL, one RGC was AGUS) or overinterpreted (one LSIL was NN). Following reassessment, false-positive staining was present in only 1/25 (1.4%) NN. Six of 30 (20%) LSIL lacked p16(INK4A) positivity. One of 10 (10%) HSIL had no staining. Two of four AGUS did not react with p16(INK4A) antibody. Both SCCA (1) and ADCA (2) had positive expression. This study confirms the intimate relationship between p16(INK4A) and HPV cytopathic effect. The p16(INK4A) immunocytochemical stain can be applied to liquid-based cervical preparations. This technique offers a more objective approach to deciphering "gray areas" of gynecologic cytopathology.
The Obesity-Associated FTO Gene Encodes a 2-Oxoglutarate-Dependent Nucleic Acid Demethylase (2007)

Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans. Here, we show by bioinformatics analysis that FTO shares sequence motifs with Fe(II)- and 2-oxoglutarate-dependent oxygenases. We find that recombinant murine Fto catalyzes the Fe(II)- and 2OG-dependent demethylation of 3-methylthymine in single-stranded DNA, with concomitant production of succinate, formaldehyde, and carbon dioxide. Consistent with a potential role in nucleic acid demethylation, Fto localizes to the nucleus in transfected cells. Studies of wild-type mice indicate that Fto messenger RNA (mRNA) is most abundant in the brain, particularly in hypothalamic nuclei governing energy balance, and that Fto mRNA levels in the arcuate nucleus are regulated by feeding and fasting. Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass.
Evaluation of serum lipid profile and cardiac enzyme changes in cerebrovascular accidents. (1995)

Serum lipid profile is, total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL) and triglycerides and serum cardiac enzymes ie, creatinine phosphokinase (CPK), creatinine phosphokinase isoenzyme MB (CPK-MB), lactate dehydrogenase (LDH) and serum aspartate aminotransferase (AST/SGOT) levels were estimated in 50 cases of cerebrovascular accidents (CVA) consisting of 26 cases of cerebral haemorrhage and 24 cases of cerebral thrombosis. All analyses were made on day 1 and day 7. Serum cholesterol, LDL and triglycerides levels were significantly higher in CVA patients on day 1. Lipid level fell significantly on day 7 in respect to day 1. On comparing the lipid levels between cerebral haemorrhage and cerebral thrombosis, no significant difference was observed. Cardiac enzymes like CPK and CPK-MB were significantly raised whereas, AST/SGOT and LDH were marginally raised on day 1 in CVA patients. However, there was no change in cardiac enzyme levels between cerebral haemorrhage and cerebral thrombosis patients.
Baculovirus expression systems and biopesticides. (1994)

Partial table of contents: DEVELOPING EFFECTIVE BACULOVIRUS--INSECT CULTURE SYSTEMS. Insect Cell Culture Methods and Their Use in Virus Research (R. Granados & K. McKenna). Comparison of Mammalian and Insect Cell Cultures (M. Shuler). Development and Testing of Genetically Improved Baculovirus Insecticides (H. Wood). BIOREACTOR DESIGN AND SCALE--UP ISSUES. Overview of Issues in Bioreactor Design and Scale--Up (R. Taticek, et al.). The Effect of Hydrodynamic Forces on Insect Cells (J. Chalmers). COMMERCIAL APPLICATION OF INSECT CELL CULTURE. Baculovirus--Mediated Production of Proteins in Insect Cells: Examples of Scale--Up and Product Recovery (M. Silberklang, et al.). Index.
An Ile-568 to Asn Polymorphism Prevents Normal Trafficking and Function of the Human P2X7 Receptor (2003)

The P2X(7) receptor is a ligand-gated channel that is highly expressed on mononuclear cells and that mediates ATP-induced apoptosis of these cells. Wide variations in the function of the P2X(7) receptor have been observed, in part because of a loss-of-function polymorphism that changes Glu-496 to Ala without affecting the surface expression of the receptor on lymphocytes. In this study a second polymorphism (Ile-568 to Asn) has been found in heterozygous dosage in three of 85 normal subjects and in three of 45 patients with chronic lymphocytic leukemia. P2X(7) function was measured by ATP-induced fluxes of Rb(+), Ba(2+), and ethidium(+) into various lymphocyte subsets and was decreased to values of approximately 25% of normal. The expression of the P2X(7) receptor on lymphocytes was approximately half that of normal values as measured by the binding of fluorescein-conjugated monoclonal antibody. Transfection experiments showed that P2X(7) carrying the Ile-568 to Asn mutation was non-functional because of the failure of cell surface expression. The differentiation of monocytes to macrophages with interferon-gamma up-regulated P2X(7) function in cells heterozygous for the Ile-568 to Asn mutation to a value around 50% of normal. These data identify a second loss-of-function polymorphism within the P2X(7) receptor and show that Ile-568 is critical to the trafficking domain, which we have shown to lie between residues 551 and 581.
A recombinant, chimeric tetravalent dengue vaccine candidate based on a dengue virus serotype 2 backbone (2015)

Dengue fever is caused by infection with one of four dengue virus (DENV) serotypes (DENV-1-4), necessitating tetravalent dengue vaccines that can induce protection against all four DENV. Takeda's live attenuated tetravalent dengue vaccine candidate (TDV) comprises an attenuated DENV-2 strain plus chimeric viruses containing the prM and E genes of DENV-1, -3 and -4 cloned into the attenuated DENV-2 'backbone'. In Phase 1 and 2 studies, TDV was well tolerated by children and adults aged 1.5-45 years, irrespective of prior dengue exposure; mild injection-site symptoms were the most common adverse events. TDV induced neutralizing antibody responses and seroconversion to all four DENV as well as cross-reactive T cell-mediated responses that may be necessary for broad protection against dengue fever.
Filter-Based Active Damping of Voltage Source Converters With $LCL$ Filter (2010)

Pulsewidth modulation (PWM) voltage source converters are becoming a popular interface to the power grid for many applications. Hence, issues related to the reduction of PWM harmonics injection in the power grid are becoming more relevant. The use of high-order filters like <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LCL</i> filters is a standard solution to provide the proper attenuation of PWM carrier and sideband voltage harmonics. However, those grid filters introduce potentially unstable dynamics that should be properly damped either passively or actively. The second solution suffers from control and system complexity (a high number of sensors and a high-order controller), even if it is more attractive due to the absence of losses in the damping resistors and due to its flexibility. An interesting and straightforward active damping solution consists in plugging in, in cascade to the main controller, a filter that should damp the unstable dynamics. No more sensors are needed, but there are open issues such as preserving the bandwidth, robustness, and limited complexity. This paper provides a systematic approach to the design of filter-based active damping methods. The tuning procedures, performance, robustness, and limitations of the different solutions are discussed with theoretical analysis, selected simulation, and experimental results.
The biochemical activities of the aggregate formed by linear gramicidin in an aqueous environment (2000)

Conference Abstract| October 01 2000 The biochemical activities of the aggregate formed by linear gramicidin in an aqueous environment V. K. Booth; V. K. Booth 1School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK Search for other works by this author on: This Site PubMed Google Scholar D. G. Flint; D. G. Flint 1School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK Search for other works by this author on: This Site PubMed Google Scholar G. F. White; G. F. White 2Department of Physics, The University of Guelph, Canada Search for other works by this author on: This Site PubMed Google Scholar F. R. Hallett; F. R. Hallett 2Department of Physics, The University of Guelph, Canada Search for other works by this author on: This Site PubMed Google Scholar Z. Zhang; Z. Zhang 3Department of Chemistry, The University of Toronto, Canada; 4 Department of Crystallography, Birkbeck College, The University of London, UK Search for other works by this author on: This Site PubMed Google Scholar G. A. Woolley; G. A. Woolley 3Department of Chemistry, The University of Toronto, Canada; 4 Department of Crystallography, Birkbeck College, The University of London, UK Search for other works by this author on: This Site PubMed Google Scholar B. A. Wallace; B. A. Wallace 3Department of Chemistry, The University of Toronto, Canada; 4 Department of Crystallography, Birkbeck College, The University of London, UK Search for other works by this author on: This Site PubMed Google Scholar O. S. Smart O. S. Smart 5 1School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK o.s.smart@bham.ac.uk Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 2000 Biochemical Society2000 Biochem Soc Trans (2000) 28 (5): A348. https://doi.org/10.1042/bst028a348 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation V. K. Booth, D. G. Flint, G. F. White, F. R. Hallett, Z. Zhang, G. A. Woolley, B. A. Wallace, O. S. Smart; The biochemical activities of the aggregate formed by linear gramicidin in an aqueous environment. Biochem Soc Trans 1 October 2000; 28 (5): A348. doi: https://doi.org/10.1042/bst028a348 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 2000 Biochemical Society2000 Article PDF first page preview Close Modal You do not currently have access to this content.
Structural Requirements for Addition of O-Linked Carbohydrate to Recombinant Erythropoietin (1994)

To define the structural requirements for addition of O-linked glycosylation in vivo, recombinant erythropoietin (rEPO) variants were constructed. Thirty-three independent Ser or Thr substitutions were constructed and examined to see which were subject to O-linked carbohydrate addition. Variants with Thr mutations at positions 123 and 125, but not elsewhere, contained additional carbohydrate, which suggests that several positions around the existing O-linked glycosylation site (Ser126), but not elsewhere, contain the necessary information for O-linked carbohydrate addition. Two forms of the Thr125 variant were identified. One form was glycosylated only at residue 125, and a second form was glycosylated at both Thr125 and Ser126, the normal O-glycosylation site. We have also found that glycosylation is less efficient when rEPO is improperly folded and that prolines at -1 and +1 relative to the O-glycosylation site enhance glycosylation.
Measurement of CO2 Diffusivity for Carbon Sequestration: A Microfluidic Approach for Reservoir-Specific Analysis (2012)

Predicting carbon dioxide (CO(2)) security and capacity in sequestration requires knowledge of CO(2) diffusion into reservoir fluids. In this paper we demonstrate a microfluidic based approach to measuring the mutual diffusion coefficient of carbon dioxide in water and brine. The approach enables formation of fresh CO(2)-liquid interfaces; the resulting diffusion is quantified by imaging fluorescence quenching of a pH-dependent dye, and subsequent analyses. This method was applied to study the effects of site-specific variables--CO(2) pressure and salinity levels--on the diffusion coefficient. In contrast to established, macro-scale pressure-volume-temperature cell methods that require large sample volumes and testing periods of hours/days, this approach requires only microliters of sample, provides results within minutes, and isolates diffusive mass transport from convective effects. The measured diffusion coefficient of CO(2) in water was constant (1.86 [± 0.26] × 10(-9) m(2)/s) over the range of pressures (5-50 bar) tested at 26 °C, in agreement with existing models. The effects of salinity were measured with solutions of 0-5 M NaCl, where the diffusion coefficient varied up to 3 times. These experimental data support existing theory and demonstrate the applicability of this method for reservoir-specific testing.
Acidic extracellular microenvironment and cancer (2013)

Acidic extracellular pH is a major feature of tumor tissue, extracellular acidification being primarily considered to be due to lactate secretion from anaerobic glycolysis. Clinicopathological evidence shows that transporters and pumps contribute to H+ secretion, such as the Na+/H+ exchanger, the H+-lactate co-transporter, monocarboxylate transporters, and the proton pump (H+-ATPase); these may also be associated with tumor metastasis. An acidic extracellular pH not only activates secreted lysosomal enzymes that have an optimal pH in the acidic range, but induces the expression of certain genes of pro-metastatic factors through an intracellular signaling cascade that is different from hypoxia. In addition to lactate, CO2 from the pentose phosphate pathway is an alternative source of acidity, showing that hypoxia and extracellular acidity are, while being independent from each other, deeply associated with the cellular microenvironment. In this article, the importance of an acidic extracellular pH as a microenvironmental factor participating in tumor progression is reviewed.
Understanding mental states in natural language (2009)

Understanding mental states in narratives is an important aspect of human language comprehension. By "mental states" we refer to beliefs, states of knowledge, points of view, and suppositions, all of which may change over time. In this paper, we propose an approach for automatically extracting and understanding multiple mental states in stories. Our model consists of two parts: (1) a parser that takes an English sentence and translates it to some semantic operations; (2) a mental-state inference engine that reads in the semantic operations and produces a situation model that represents the meaning of the sentence. We present the performance of the system on a corpus of children stories containing both fictional and non-fictional texts.
Deep‐sea measurements without wires or cables (1938)

In the deep‐sea seismic measurements which were commenced last summer on the Atlantis , the principal problems for which no good solution is in sight are connected with the cable. It seems likely that the best solution is to dispense with the cable altogether. Figure 1 shows the apparatus as used last year. It consists of a series of bombs, a series of seismographs, and an oscillograph‐chamber. The cable leading from the ship to the oscillograph is a wire rope which serves only to lower and hoist the apparatus. It is necessary to place the 600‐pound oscillograph‐chamber on the bottom of the ocean in such a way that the 4200‐foot string of seismographs and bombs shall lead away from it in a line which is very nearly straight. The actual distances from each bomb to each seismograph are determined from the travel‐time of the water‐wave. Only three tests have been made to date, but it is revealed by the water‐waves that the line of instruments did not extend to nearly its full length in any test. In one test the 20‐pound bomb was actually less than 600 feet from the oscillograph. Perhaps further practice in the maneuver of laying out the apparatus would overcome this difficulty to some extent, but it will always be difficult to stretch this line out straight without parting it. This is the first problem to be solved.
Peptide Conjugation to a Polymer Coating via Native Chemical Ligation of Azlactones for Cell Culture (2016)

Conjugation of biomolecules for stable presentation is an essential step toward reliable chemically defined platforms for cell culture studies. In this work, we describe the formation of a stable and site-specific amide bond via the coupling of a cysteine terminated peptide at low concentration to an azlactone containing copolymer coating. A copolymer of polyethylene glycol methyl ether methacrylate-ran-vinyl azlactone-ran-glycidyl methacrylate P(PEGMEMA-r-VDM-r-GMA) was used to form a thin coating (20-30 nm) on silicon and polycarbonate substrates. The formation and stability of coating-peptide bonds for peptides containing free thiols and amines were quantified by X-ray photoelectron spectroscopy (XPS) after exposure to cell culture conditions. Peptides containing a thiol as the only nucleophile coupled via a thioester bond; however, the bond was labile under cell culture conditions and almost all the bound peptides were displaced from the surface over a period of 2 days. Coupling with N-terminal primary amine peptides resulted in the formation of an amide bond with low efficiency (<20%). In contrast, peptides containing an N-terminal cysteine, which contain both nucleophiles (free thiol and amine) in close proximity, bound with 67% efficiency under neutral pH, and were stable under the same conditions for 2 weeks. Control studies confirm that the stable amide formation was a result of an intramolecular rearrangement through a N-acyl intermediate that resembles native chemical ligation. Through a combination of XPS and cell culture studies, we show that the cysteine terminated peptides undergo a native chemical ligation process at low peptide concentration in aqueous media, short reaction time, and at room temperature resulting in the stable presentation of peptides beyond 2 weeks for cell culture studies.