gebhart/patrepeval huggingface dataset:
A case of a novel PML/RARA short fusion transcript with truncated transcription variant 2 of the RARA gene. (2010)

Acute promyelocytic leukemia (APL) with atypical breakpoints in the promyelocytic leukemia (PML) and retinoic acid receptor-alpha (RARA) genes represents a rare leukemic event, which occurs preferentially in patients with variant types of the PML/RARA fusion gene. Here we report on a patient with APL with a unique PML/RARA fusion transcript that harbors a short type of this fusion gene, exhibiting unexpected results of standard PCR diagnostics. The detected transcript originates from fusion of PML exon 4 and a truncated form of transcription variant 2 of the RARA gene, with an additional 9 bp insertion. According to our knowledge, this differs from all previously described fusion transcripts.
Epigenetics and epigenetic alterations in pancreatic cancer. (2009)

Pancreatic cancer remains a major therapeutic challenge. In 2008, there will be approximately 37,680 new cases and 34,290 deaths attributable to pancreatic cancer in the United States (U.S.), making it the fourth leading cause of cancer-related death. Recent comprehensive pancreatic cancer genome project found that pancreatic adenocarcinomas harbored 63 intragenic mutations or amplifications/homozygous deletions and these alterations clustered in 12 signaling pathways. In addition to widespread genetic alterations, it is now apparent that epigenetic mechanisms are also central to the evolution and progression of human cancers. Since epigenetic silencing processes are mitotically heritable, they can drive neoplastic progression and undergo the same selective pressure as genetic alterations. This review will describe recent developments in cancer epigenetics and their importance in our understanding of pancreatic adenocarcinomas.
2,2‐Difluoro‐1,3‐dimethylimidazolidine (DFI). A New Fluorinating Agent. (2002)

Abstract For Abstract see ChemInform Abstract in Full Text.
Fully 3-Dimensional NOR Flash Cell with Recessed Channel and Cylindrical Floating Gate - A Scaling Direction for 65nm and Beyond (2006)

For the first time, a fully 3-dimensional (3-D) flash cell with recessed channel and cylindrical floating gate is successfully integrated with 65nm MLC NOR flash technology. Key process technologies to achieve the novel cell structures are shown to be highly feasible. Superior scalability and comparable device characteristics including V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sub> distribution and tunnel oxide reliability are proved through 512Mb full chip integration. Intrinsic immunity on the short channel effect of this 3-D cell opens a promising path for the continued scaling of the floating gate flash memories for 65nm and beyond
Does Total Knee Replacement Restore Normal Knee Function? (2005)

Despite the advanced age of many patients having total knee arthroplasty, previous attempts to quantify patient function postoperatively have not allowed for normal deterioration of musculoskeletal function that occurs with aging. We determined the effects of aging on knee function, thereby providing a realistic level of normal, healthy knee function for patients and surgeons after total knee arthroplasties. A self-administered, validated knee function questionnaire consisting of 55 scaled multiple choice questions was used in this study. Responses were collected from 243 patients at least 1 year after they had total knee arthroplasties, and from 257 individuals (age- and gender-matched) who had no previous history of knee disorders. Many of these latter subjects reported that they could do most of the activities cited in the questionnaire without symptoms attributable to their knees. However, knee symptoms were experienced more frequently during activities that placed greater loads on the extremity. There was no difference in the knee function of men and women, and both groups had continuous deterioration in knee function with increasing age. There were large differences in the functional capacity to do activities involving the knee between the group of patients who had total knee arthroplasties and the age- and gender-matched patients with no previous knee disorders. Overall, 52% of the patients who had total knee arthroplasties reported some degree of limitation in doing functional activities, versus 22% of subjects with no previous knee disorders. Two groups of activities were identified: activities in which the patients and control subjects had essentially similar knee function (swimming, golfing, and stationary biking), and activities in which the function scores of the control group exceeded the scores of the patients who had total knee arthroplasties (kneeling, squatting, moving laterally, turning and cutting, carrying loads, stretching, leg strengthening, tennis, dancing, gardening, and sexual activity). Our data show that many of the limitations reported by patients after total knee arthroplasties are shared by individuals with no previous knee disorders. However, only approximately 40% of the functional deficit present after a total knee arthroplasty seems to be attributable to the normal physiologic effects of aging. Patients who had total knee replacements still experienced substantial functional impairment compared with their age- and gender-matched peers, especially when doing biomechanically demanding activities. This suggests that significant improvements in the procedure and prosthetic designs are needed to restore normal knee function after a total knee arthroplasty.
PCR‐based detection of resistance to acetyl‐CoA carboxylase‐inhibiting herbicides in black‐grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud) (2002)

A simple method based upon allele-specific PCR was developed to detect an isoleucine-leucine substitution in the gene encoding chloroplastic acetyl-coenzyme A carboxylase (ACCase) in two gramineous weeds: Lolium rigidum Gaud and Alopecurus myosuroides Huds. Analysis of 1800 A myosuroides and 750 L rigidum seedlings showed that the presence of ACCase leucine allele(s) conferred cross-resistance to the cyclohexanedione herbicide cycloxydim and to the aryloxyphenoxypropionate herbicides fenoxaprop-P-ethyl and diclofop-methyl. Seedlings containing ACCase leucine allele(s) could be either sensitive or resistant to the aryloxyphenoxypropionate herbicides haloxyfop-P-methyl and clodinafop-propargyl. Successful detection of resistant plants in a field population of A myosuroides was achieved using this PCR assay. Using it with basic molecular biology laboratory equipment, the presence of resistant leucine ACCase allele(s) can be detected within one working day.
Unique role of the complement receptor CR1 in the degradation of C3b associated with immune complexes. (1982)

The main finding of this paper is that CR1, the membrane receptor for C3b and C4b, together with C3b/C4b-inactivator (I), degrades C3b bound to immune complexes (C3b*). Two fragments are generated: C3c, which is released from the immune complexes, and C3d*. The C3c fragment released from the cell intermediate EAC1423b prepared with 125I-C3 was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and radioautography. It has a 135,000 mol wt and contains disulfide bonded labeled polypeptide chains of 75,000 and 31,000 mol wt, which presumably represent the beta and a fragment of the alpha-chain of C3b*. Silver staining of the SDS-PAGE gels revealed other C3-derived bands with 39-42,000 mol wt. Human erythrocytes + I also cleave C3b* into C3c and C3d*. The activity of the erythrocytes is CR1 mediated because it can be totally inhibited by monoclonal antibodies to CR1. In contrast with these results, I together with the serum protein beta 1H (H) transform EAC1423b into hemolytically inactive EAC1423bi and cleave the alpha' chain of C3b* into fragments of 70,000 and 40,000 mol wt. Small amounts of C3c are also released at relatively high concentrations of H. On a molar basis, the efficiency of CR1 in the generation of C3c and C3d is 10(4)-10(5) greater than H. An additional observation was that C3c could be released by treating EAC1423bi with CR1 + I and that this reaction was also inhibited by monoclonal antibodies to CR1. Therefore, it is likely that CR1 has binding affinity for iC3b and that the degradation of C3b* proceeds as follows: C3b (formula, see text) C3c + C3d*. Taken together, our findings argue that the processing of C3b* in vivo occurs in solid phase, that is, on the surface of cells bearing CR1.
Applications of self-interference cancellation in 5G and beyond (2014)

Self-interference cancellation invalidates a long-held fundamental assumption in wireless network design that radios can only operate in half duplex mode on the same channel. Beyond enabling true in-band full duplex, which effectively doubles spectral efficiency, self-interference cancellation tremendously simplifies spectrum management. Not only does it render entire ecosystems like TD-LTE obsolete, it enables future networks to leverage fragmented spectrum, a pressing global issue that will continue to worsen in 5G networks. Self-interference cancellation offers the potential to complement and sustain the evolution of 5G technologies toward denser heterogeneous networks and can be utilized in wireless communication systems in multiple ways, including increased link capacity, spectrum virtualization, any-division duplexing (ADD), novel relay solutions, and enhanced interference coordination. By virtue of its fundamental nature, self-interference cancellation will have a tremendous impact on 5G networks and beyond.
Localized induction heating solder bonding for wafer level MEMS packaging (2004)

This paper reports a new solder bonding method for the wafer level packaging of MEMS devices. The electroplated magnetic film was heated up using the induction heating, and leaded to solder reflow. It took only several seconds to complete the solder reflow and bonding process. The measurement results showed that the temperature of device region was only 110/spl deg/C during heating. Due to the solder reflow and electroplating technique, hermetic seal of the packaged device is achieved. The bonding strength is up to 18 MPa. In addition, the electroplated thick film also performed as a spacer. In applications, the integration the MUMPs devices with the proposed packaging techniques were demonstrated.
Effective Rejection of Fluorescence Interference in Raman Spectroscopy Using a Shifted Excitation Difference Technique (1992)

Resonance Raman spectroscopy is a powerful technique for probing the vibrations of particular chromophores in multicomponent systems. By tuning the wavelength of the excitation laser into resonance with an electronic absorption band of only one molecular species, the vibrational Raman scattering from this species can be selectively enhanced. Thus, resonance Raman spectroscopy can provide structural information for chromophores in solution or biological chromophores within their functionally active protein environment. However, since the very nature of the experiment requires that the excitation light be absorbed by the sample, the measurement of resonance Raman spectra is often made difficult by a large fluorescence background in the same spectral region as the Raman scattering. The problem of fluorescence interference from intrinsic sample emission is often further exacerbated in biological samples, where low-concentration impurities with large fluorescence yields can be difficult to remove. Even weak fluorescence, with an effective fluorescence quantum yield of ≈10−4, completely overwhelms resonance Raman signals, which have typical quantum yields of ≈10−7.
Extraordinary optoconductance in metal-semiconductor hybrid structures (2006)

We provide the first demonstration of extraordinary optoconductance (EOC) in macroscopic metal-semiconductor hybrid structures fabricated from GaAs and In and the first example of an extraordinary transport (EXX) effect that is based on a perturbation of the relative bulk conductances of the constituents. Four-lead van der Pauw plate structures show a gain of order 500% in the optocondutance when shunted by In relative to unshunted devices. The dependence of the optocondutance on the position of a focussed Ar laser beam has been measured and is in agreement with a Gaussian broadened point charge model that accounts for the Dember effect. We also account quantitatively for the temperature dependence of the EOC.
Substituent effects of iridium complexes for highly efficient red OLEDs (2005)

This study reports substituent effects of iridium complexes with 1-phenylisoquinoline ligands. The emission spectra and phosphorescence quantum yields of the complexes differ from that of tris(1-phenylisoquinolinato-C2,N)iridium(iii)(Irpiq) depending on the substituents. The maximum emission peak, quantum yield and lifetime of those complexes ranged from 598-635 nm, 0.17-0.32 and 1.07-2.34 micros, respectively. This indicates the nature of the substituents has a significant influence on the kinetics of the excited-state decay. The substituents attached to phenyl ring have an influence on a stability of the HOMO. Furthermore, those substituents have effect on the contribution to a mixing between 3pi-pi* and (3)MLCT for the lowest excited states. Some of the complexes display the larger quantum yield than Irpiq, which has the quantum yield of 0.22. The organic light emitting diode (OLED) device based on tris [1-(4-fluoro-5-methylphenyl)isoquinolinato-C2,N]iridium(iii)(Ir4F5Mpiq) yielded high external quantum efficiency of 15.5% and a power efficiency of 12.4 lm W(-1) at a luminance of 218 cd m(-2). An emission color of the device was close to an NTSC specification with CIE chromaticity characteristics of (0.66, 0.34).
Active and stable carbon nanotube/nanoparticle composite electrocatalyst for oxygen reduction (2013)

Nanostructured carbon-based materials, such as nitrogen-doped carbon nanotube arrays, Co3O4/nitrogen-doped graphene hybrids and carbon nanotube-graphene complexes have shown respectable oxygen reduction reaction activity in alkaline media. Although certainly promising, the performance of these materials does not yet warrant implementation in the energy conversion/storage devices utilizing basic electrolytes, for example, alkaline fuel cells, metal-air batteries and certain electrolysers. Here we demonstrate a new type of nitrogen-doped carbon nanotube/nanoparticle composite oxygen reduction reaction electrocatalyst obtained from iron acetate as an iron precursor and from cyanamide as a nitrogen and carbon nanotube precursor in a simple, scalable and single-step method. The composite has the highest oxygen reduction reaction activity in alkaline media of any non-precious metal catalysts. When used at a sufficiently high loading, this catalyst also outperforms the most active platinum-based catalysts.
Vi capsular polysaccharide-protein conjugates for prevention of typhoid fever. Preparation, characterization, and immunogenicity in laboratory animals. (1987)

The Vi has proven to be a protective antigen in two double masked, controlled clinical trials in areas with high rates of typhoid fever (approximately 1% per annum). In both studies the protective efficacy of the Vi was approximately 70%. Approximately 75% of subjects in these areas responded with a fourfold or greater rise of serum Vi antibodies. In contrast, the Vi elicited a fourfold or greater rise in 95-100% of young adults in France and the United States. Methods were devised, therefore, to synthesize Vi-protein conjugates in order to both enhance the antibody response and confer T-dependent properties to the Vi (and theoretically increase its protective action in populations at high risk for typhoid fever). We settled on a method that used the heterobifunctional crosslinking reagent, N-succinimidyl-3-(2-pyridyldithio)-propionate (SPDP), to bind thiol derivatives of the Vi to proteins. This synthetic scheme was reproducible, provided high yields of Vi-protein conjugates, and was applicable to several medically relevant proteins such as diphtheria and tetanus toxoids. The resultant conjugates were more immunogenic in mice and juvenile Rhesus monkeys than the Vi alone. In contrast to the T-independent properties of the Vi, conjugates of this polysaccharide with several medically relevant proteins induced booster responses in mice and in juvenile Rhesus monkeys. Clinical studies with Vi-protein conjugates are planned. This scheme is also applicable to synthesize protein conjugates with other polysaccharides that have carboxyl functions.
Unique Biology of Mcl-1: Therapeutic Opportunities in Cancer (2008)

Accumulating evidence suggests that Mcl-1 plays a critical pro-survival role in the development and maintenance of both normal and malignant tissues. Regulation of Mcl-1 expression occurs at multiple levels, allowing for either the rapid induction or elimination of the protein in response to different cellular events. This suggests that Mcl-1 can play an early role in response to signals directing either cell survival or cell death. Deregulation of pathways regulating Mcl-1 that result in its over-expression likely contribute to a cell's inability to properly respond to death signals possibly leading to cell immortalization and tumorigenic conversion. Correspondingly, Mcl-1 has been shown to be up-regulated in numerous hematological and solid tumor malignancies. Moreover, this up-regulation appears to be a factor in the resistance of some cancer types to conventional cancer therapies. Mechanisms that abrogate the pro-survival function of Mcl-1 either by diminishing its levels or inactivating its functional BH3 groove have shown promise for the combinational treatment with existing cancer therapies and as single agents in certain malignancies. Here we review the various pathways that regulate Mcl-1 expression and describe agents that are currently under development to modulate Mcl-1 activity for therapeutic benefit in oncology.
Histamine H3 ligands: just pharmacological tools or potential therapeutic agents? (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHistamine H3 ligands: just pharmacological tools or potential therapeutic agents?Hendrik TimmermanCite this: J. Med. Chem. 1990, 33, 1, 4–11Publication Date (Print):January 1, 1990Publication History Published online1 May 2002Published inissue 1 January 1990https://pubs.acs.org/doi/10.1021/jm00163a001https://doi.org/10.1021/jm00163a001research-articleACS PublicationsRequest reuse permissionsArticle Views240Altmetric-Citations82LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Purification of gases in H-mordenite modified with silane and diborane (1983)

Silane and diborane have been found to be very suitable for the structural modification of H-zeolites, and stepwise boranation of the H-mordenite structure has been studied. The effect of the degree of modification on the pore size and the affinity of the zeolite was checked by sorption experiments with N2(77 K) and Xe and Kr (273 K). A further narrowing of the pore size was achieved by oxidation of the implanted (BH)x groups with H2O or other molecules such as CH3OH. The pore size could be ‘engineered’ in a controlled way so that it could be applied to finely tuned separations, yielding components of pure Kr from Kr + N2, Ar + Kr, O2+ Kr and N2+ O2+ Kr, pure Ar from Ar + Kr, pure N2 from N2+ Kr, pure O2 from O2+ Kr and pure CH4 from H2+ N2+ CH4 mixtures.
Lysine- and arginine-specific proteinases from Porphyromonas gingivalis. Isolation, characterization, and evidence for the existence of complexes with hemagglutinins. (1994)

Porphyromonas gingivalis contains many virulence factors that have been implicated as participants in the progression of periodontal disease. It has been shown to produce proteinases of "trypsin-like" specificity in a number of molecular forms, but previous work in our laboratory resulted in the purification of a major arginine-specific cysteine proteinase, gingipain, which contradicted this supposed specificity. In this study, separate proteinases with arginine and lysine specificity were isolated from a high molecular mass fraction of the P. gingivalis culture fluid. The arginine-specific enzyme was found, by amino acid sequencing studies, to be a high molecular mass form of gingipain, formed by the 50-kDa gingipain noncovalently complexed with 44-kDa binding proteins, subsequently identified as hemagglutinins. The 60-kDa lysine-specific proteinase, referred to as Lys-gingipain, was also found to have one of these hemagglutinins complexed with it in the same manner. Lys-gingipain was found to be a cysteine proteinase with optimal activity and stability at pH 8.0-8.5 and was extensively characterized in terms of its specificity and activation characteristics. The proteinase-hemagglutinin complexes may be important in the uptake of hemin, a vital metabolite for P. gingivalis, via hemagglutination and subsequent hemolysis of erythrocytes.
Nanoscale separation of molecular species based on their rotational mobility (2008)

We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.
Template-directed synthesis: use of a reversible reaction (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTemplate-directed synthesis: use of a reversible reactionJay T. Goodwin and David G. LynnCite this: J. Am. Chem. Soc. 1992, 114, 23, 9197–9198Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November 1992https://pubs.acs.org/doi/10.1021/ja00049a067https://doi.org/10.1021/ja00049a067research-articleACS PublicationsRequest reuse permissionsArticle Views1082Altmetric-Citations125LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts