gebhart/patrepeval huggingface dataset:
Diversity interconnection of wireless terminals to local area networks via radio bridges (1992)

The Letter considers the problem of interconnecting wireless terminals to a local area network (LAN) through multiple radio bridges. A network architecture is presented which enables flexible relocation of terminals sharing the same pair of radio channels over the network. The bridge algorithms selflearn for frame forwarding, and take advantage of multiple radio paths to combat propagation adversities while preventing frame duplication over the LAN.
Biospecific Adsorption of Carbonic Anhydrase to Self-Assembled Monolayers of Alkanethiolates That Present Benzenesulfonamide Groups on Gold (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiospecific Adsorption of Carbonic Anhydrase to Self-Assembled Monolayers of Alkanethiolates That Present Benzenesulfonamide Groups on GoldMilan Mrksich, Jocelyn R. Grunwell, and George M. WhitesidesCite this: J. Am. Chem. Soc. 1995, 117, 48, 12009–12010Publication Date (Print):December 1, 1995Publication History Published online1 May 2002Published inissue 1 December 1995https://pubs.acs.org/doi/10.1021/ja00153a029https://doi.org/10.1021/ja00153a029research-articleACS PublicationsRequest reuse permissionsArticle Views678Altmetric-Citations128LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cellular and extracellular involvement in the regeneration of the rat lower vibrissa follicle (1992)

The sequence of events leading to the reconstruction of a fibre-producing hair follicle, after microsurgical amputation of the lower follicle bulb, has been detailed by immunohistology and electron microscopy. The initial response was essentially found to be a wound reaction, in that hyperproliferative follicle epidermis quickly spread to below the level of amputation--associated with downward movement of mesenchymal (or dermal) sheath cells. Fibronectin was prominent in both dermis and epidermis at this stage and, as in wound repair, preceded laminin and type IV collagen in covering the lower dermal-epidermal junction. Once a new basal line of epidermis and a complete basement membrane were established, laminin and type IV collagen were detected below this junction and within the prospective papilla-forming mesenchyme. This coincided with ultrastructural observations of profuse sub-basement membrane extracellular material in the region of new papilla formation. The glassy membrane displayed extensive ultrastructural modifications at its lower level, and these corresponded with localized variations in staining intensities for all three antibodies over time. The membrane hung below the level of the epidermis, and was crossed by migrating cells from the mesenchymal dermal sheath of the follicle - it acted to segregate the inner group of follicular dermal cells from wound fibroblasts. Extracellular matrix may be a mediator of the dermal-epidermal interactions associated with this hair follicle regeneration phenomenon.
The Basis for the Immunoregulatory Role of Macrophages and Other Accessory Cells (1987)

Macrophages handle extracellular proteins and secrete diverse bioactive molecules and, therefore, influence the physiology of many tissues. They also have an important immunoregulatory role. The immune response to proteins involves the activation of the T helper subset of lymphocytes. The T helper cell is activated only when it interacts with the protein displayed on the surface of a macrophage or other accessory cell. This interaction involves restrictive proteins encoded in the major histocompatibility gene complex as well as growth-differentiating proteins.
Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations (1992)

Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or pyridoxal led to the isolation of insertions in pdxA, pdxB, and pdxH but not in pdxJ. This observation suggested that pdxJ, like pdxA, pdxB, and serC, might be in a complex operon. To test this hypothesis, we constructed stable insertion mutations in and around pdxJ in plasmids and forced them into the bacterial chromosome. Physiological properties of the resulting insertion mutants were characterized, and the DNA sequence of pdxJ and adjacent regions was determined. These combined approaches led to the following conclusions: (i) pdxJ is the first gene in a two-gene operon that contains a gene, temporarily designated dpj, essential for Escherichia coli growth; (ii) expression of the rnc-era-recO and pdxJ-dpj operons can occur independently, although the pdxJ-dpj promoter may lie within recO; (iii) pdxJ encodes a 26,384-Da polypeptide whose coding region is preceded by a PDX box, and dpj probably encodes a basic, 14,052-Da polypeptide; (iv) mini-Mud insertions in dpj and pdxJ, which are polar on dpj, severely limit E. coli growth; and (v) three classes of suppressors, including mutations in lon and suppressors of lon, that allow faster growth of pdxJ::mini-Mud mutants can be isolated. A model to account for the action of dpj suppressors is presented, and aspects of this genetic analysis are related to the pyridoxal 5'-phosphate biosynthetic pathway.
ProApolipoprotein A1 (2008)

In conclusion, these data support the use of serum markers for the early detection of brain metastases. ProApolipoprotein A1 may be used in conjunction with S100beta for serum-based, MRI-independent diagnosis of metastatic brain tumors.
A hybrid twin screw extrusion/electrospinning method to process nanoparticle-incorporated electrospun nanofibres (2008)

A new hybrid methodology that fully integrates the processing capabilities of the twin screw extrusion process (conveying solids, melting, dispersive and distributive mixing, pressurization, temperature profiling, devolatilization) with electrospinning is described. The hybrid process is especially suited to the dispersion of nanoparticles into polymeric binders and the generation of nanoparticle-incorporated fibres and nanofibres. The new technology base is demonstrated with the dispersion of β-tricalcium phosphate (β-TCP) nanoparticles into poly(ε-caprolactone) (PCL) to generate biodegradable non-woven meshes that can be targeted as scaffolds for tissue engineering applications. The new hybrid method yielded fibre diameters in the range of 200-2000 nm for both PCL and β-TCP/PCL (35% by weight) composite scaffolds. The degree of crystallinity of polycaprolactone meshes could be manipulated in the 35.1-41% range, using the voltage strength as a parameter. The electrospinning process, integrated with dispersive kneading disc elements, facilitated the decrease of the cluster sizes and allowed the continuous compounding of the nanoparticles into the biodegradable polymer prior to electrospinning. Thermogravimetric analysis (TGA) of the non-woven meshes validated the continuous incorporation of 35 ± 1.5% (by weight) β-TCP nanoparticles for a targeted concentration of 35%. Uniaxial tensile testing of the meshes with and without the nanoparticles indicated that the ultimate tensile strength at break of the meshes increased from 0.47 ± 0.04 to 0.79 ± 0.08 MPa upon the incorporation of the β-TCP nanoparticles. This demonstration study suggests that the new technology base is particularly suitable for the concomitant dispersion and electrospinning of nanoparticles in the generation of myriad types of functional nanofibres.
Rigid Luminescent Bis‐Zinc(II)–Bis‐Cyclen Complexes for the Detection of Phosphate Anions and Non‐Covalent Protein Labeling in Aqueous Solution (2011)

Abstract A series of water‐soluble bis‐ and tetrakis‐Zn II –cyclen complexes with rigid structures was prepared to enhance the carboxylate and phosphate ion binding response in contrast to analogues with less‐confined molecular structures. Boc‐protected 6‐chloro‐1,3,5‐triazine–bis‐cyclen was coupled to different aryl and alkyl moieties in moderate to high yields and subsequently converted into the corresponding bis‐ or tetrakis‐Zn II –cyclen complexes. The bis‐Zn II –cyclen moiety is known for its affinity to anions. Depending on the arene substituent some of the synthesized synthetic receptors are luminescent. They were studied by absorption and emission spectroscopy for their response to the presence of phosphate anions of biological relevance in buffered aqueous solution at neutral pH and for their affinity to the genetically encodable oligoaspartate and glutamate sequences (D 4 and E 4 tag) recently introduced. The rigid structures of the compounds enhance the electronic coupling between the metal complex binding site and the reporter dye. This leads to an increased anion binding response in homogeneous aqueous solution.
Staphylococcus epidermidis Strategies to Avoid Killing by Human Neutrophils (2010)

Staphylococcus epidermidis is a leading nosocomial pathogen. In contrast to its more aggressive relative S. aureus, it causes chronic rather than acute infections. In highly virulent S. aureus, phenol-soluble modulins (PSMs) contribute significantly to immune evasion and aggressive virulence by their strong ability to lyse human neutrophils. Members of the PSM family are also produced by S. epidermidis, but their role in immune evasion is not known. Notably, strong cytolytic capacity of S. epidermidis PSMs would be at odds with the notion that S. epidermidis is a less aggressive pathogen than S. aureus, prompting us to examine the biological activities of S. epidermidis PSMs. Surprisingly, we found that S. epidermidis has the capacity to produce PSMδ, a potent leukocyte toxin, representing the first potent cytolysin to be identified in that pathogen. However, production of strongly cytolytic PSMs was low in S. epidermidis, explaining its low cytolytic potency. Interestingly, the different approaches of S. epidermidis and S. aureus to causing human disease are thus reflected by the adaptation of biological activities within one family of virulence determinants, the PSMs. Nevertheless, S. epidermidis has the capacity to evade neutrophil killing, a phenomenon we found is partly mediated by resistance mechanisms to antimicrobial peptides (AMPs), including the protease SepA, which degrades AMPs, and the AMP sensor/resistance regulator, Aps (GraRS). These findings establish a significant function of SepA and Aps in S. epidermidis immune evasion and explain in part why S. epidermidis may evade elimination by innate host defense despite the lack of cytolytic toxin expression. Our study shows that the strategy of S. epidermidis to evade elimination by human neutrophils is characterized by a passive defense approach and provides molecular evidence to support the notion that S. epidermidis is a less aggressive pathogen than S. aureus.
Ku-band MMIC phase shifter using a parallel resonator with 0.18-/spl mu/m CMOS technology (2006)

A digital 5-bit phase shifter at Ku-band is presented, which is implemented with 0.18-mum RFCMOS technology. n-MOSFET switches and top metal microstrip lines with a first-metal ground allow the phase shifter to have small insertion losses. The proposed 90deg phase shifter utilizing a parallel resonator exhibits broad-band characteristics. All of the circuit components are derived to obtain a minimum phase variation at the operation frequency band. A bridged-T type phase shifter is also analyzed in view of parallel resonance using an ideal equivalent-circuit model. The conditions of the circuit elements are derived in an analytic form, which are used to obtain the broad-band phase characteristics. The fabricated 5-bit phase shifter demonstrates an overall rms phase error less than 12deg from 9 to 15 GHz. Insertion losses of 14.5 dB plusmn 0.5 dB and return losses less than 14 dB are obtained for 32 states at 12 GHz. The proposed 90deg phase shifter has performed a phase shift of 92.3degplusmn3.2deg over 9-15 GHz
Triangulations and meshes in computational geometry (2000)

The Delaunay triangulation of a finite point set is a central theme in computational geometry. It finds its major application in the generation of meshes used in the simulation of physical processes. This paper connects the predominantly combinatorial work in classical computational geometry with the numerical interest in mesh generation. It focuses on the two- and three-dimensional case and covers results obtained during the twentieth century.
Fetal electrocardiogram extraction by source subspace separation (1995)

A high-precision method is presented for the extraction of the fetal electrocardiogram from multi-channel potential recordings on the mother&amp;apos;s skin. First the problem is appropriately formulated in terms of blind source separation, where the sources can have a multidimensional nature. As a summary of preceding work it is shown how the fetal electrocardiogram can be reconstructed by means of second-order tools. However an extra higher-order processing step enhances the accuracy and allows to identify the transfer from source to electrode. The method is illustrated with an example. 1.
Protein Purification by Affinity Chromatography (1970)

The preparation of a number of agarose and polyacrylamide bead derivatives useful in the purification of proteins by affinity chromatography is described. These techniques permit (a) the attachment of ligands to the gel through extended hydrocarbon chains which place the ligand at varying distances from the gel matrix backbone; (b) the covalent attachment of ligands to agarose or polyacrylamide gels through amino, carboxyl, phenolic, or imidazole groups of the ligand; and (c) the preparation of adsorbents containing ligands attached by bonds which are susceptible to specific chemical cleavage, thus providing means of removing the intact protein-ligand complex from the affinity adsorbent. It is demonstrated that successful application of affinity chromatography in many cases will critically depend on placing the ligand at a considerable distance from the matrix backbone. Techniques are also described which provide important approaches and considerations in the insolubilization of peptides and proteins to agarose and polyacrylamide.
Genome-wide survey of V-ATPase genes inDrosophilareveals a conserved renal phenotype for lethal alleles (2005)

V-ATPases are ubiquitous, vital proton pumps that play a multiplicity of roles in higher organisms. In many epithelia, they are the major energizer of cotransport processes and have been implicated in functions as diverse as fluid secretion and longevity. The first animal knockout of a V-ATPase was identified in Drosophila, and its recessive lethality demonstrated the essential nature of V-ATPases. This article surveys the entire V-ATPase gene family in Drosophila, both experimentally and in silico. Adult expression patterns of most of the genes are shown experimentally for the first time, using in situ hybridization or reporter gene expression, and these results are reconciled with published expression and microarray data. For each subunit, the single gene identified previously by microarray, as upregulated and abundant in tubules, is shown to be similarly abundant in other epithelia in which V-ATPases are known to be important; there thus appears to be a single dominant "plasma membrane" V-ATPase holoenzyme in Drosophila. This provides the most comprehensive view of V-ATPase expression yet in a multicellular organism. The transparent Malpighian tubule phenotype first identified in lethal alleles of vha55, the gene encoding the B-subunit, is shown to be general to those plasma membrane V-ATPase subunits for which lethal alleles are available, and to be caused by failure to accumulate uric acid crystals. These results coincide with the expression view of the gene family, in which 13 of the genes are specialized for epithelial roles, whereas others have spatially or temporally restricted patterns of expression.
A hybrid-ring method of simulating higher powers than are available in waveguides (1954)

A method is described whereby a waveguide component can be subjected to an effective power which is greater than that actually available from the magnetron; with a single hybrid ring the effective power is twice that available, and when n such rings are used the power multiplication factor is 2n.
Synthesis of alkylating oligonucleotide derivatives containing cholesterol or phenazinium residues at their 3′‐terminus and their interaction with DNA within mammalian cells (1989)

5'-[32P]-labelled alkylating decathymidylate [4-(N-2-chloroethyl)N-methylaminobenzyl]-5'-phosphamide derivatives containing cholesterol or phenazinium residues at their 3'-termini were synthesized and used for alkylation of DNA within mammalian cells. The uptake of the cholesterol derivative by the cells and the extent of DNA alkylation are about two orders of magnitude higher than those of a similar alkylating derivative lacking the groups at the 3'-termini. The presence of the phenazinium residue at the 3'-terminus of the oligonucleotide reagent does not improve the reagent uptake by the cells but drastically increases the DNA modification efficiency.
Si/Ge nanostructures (2001)

A review is given on the formation mechanisms and the properties of Si/Ge nanostructures that have been synthesized by self-assembling and self-ordering during heteroepitaxy of \mbox{silicon-germanium} alloys on single-crystal silicon substrates. The properties of electronic subbands in smooth strained Si/SiGe quantum well structures are presented as a basis for characterizing coherent Si/Ge nanostructures with free motion of carriers in a reduced number of dimensions. The low-dimensional band structure of valence band states confined in strained Si/Ge and Si/SiGe nanostructures is analysed by optical and electrical spectroscopy.
Prevalence of anthelmintic resistance on 62 beef cattle farms in the North Island of New Zealand (2006)

Anthelmintic resistance in parasitic nematodes of cattle is common in the North Island of New Zealand. Beef farmers need to be aware of the risks posed by anthelmintic resistance, and routine FECR testing is recommended to ensure optimal productivity and to guide decision-making when purchasing anthelmintics to be used on-farm.
The use of the dried blood spot sample in epidemiological studies. (1999)

A case of pulmonary artery perforation by a flow-directed balloon-tipped catheter necessitated pneumonectomy. Predisposing conditions are discussed and conclusions made on the basis of this case and previous reports.
Matched-filter interrogation technique for fibreBragggrating arrays (1995)

A technique for the demodulation of fibre Bragg grating (FBG) sensor arrays that uses a matched grating array to optically filter and track wavelength shifts from the FBG sensors is demonstrated. The use of matched grating arrays in the serial configuration represents an efficient and potentially low-cost interrogation technique for FBG sensors.
Synthesis of new carbocyclic analogues of 3′-azido- and 3′-amino-2′,3′-dideoxynucleosides (1990)

A route is described for the synthesis of cyclopentane analogues of 1-(3′-azido- and 3′-amino-2′,3′-dideoxy-β-erythro-pentofuranosyl)-5-iodouracil and -(E)-5-(2-bromovinyl)uracil, compounds of interest as potential antiviral and/or antitumour agents.
Tissue Engineering of Vascularized Cardiac Muscle From Human Embryonic Stem Cells (2007)

Transplantation of a tissue-engineered heart muscle represents a novel experimental therapeutic paradigm for myocardial diseases. However, this strategy has been hampered by the lack of sources for human cardiomyocytes and by the scarce vasculature in the ischemic area limiting the engraftment and survival of the transplanted muscle. Beyond the necessity of endothelial capillaries for the delivery of oxygen and nutrients to the grafted muscle tissue, interactions between endothelial and cardiomyocyte cells may also play a key role in promoting cell survival and proliferation. In the present study, we describe the formation of synchronously contracting engineered human cardiac tissue derived from human embryonic stem cells containing endothelial vessel networks. The 3D muscle consisted of cardiomyocytes, endothelial cells (ECs), and embryonic fibroblasts (EmFs). The formed vessels were further stabilized by the presence of mural cells originating from the EmFs. The presence of EmFs decreased EC death and increased EC proliferation. Moreover, the presence of endothelial capillaries augmented cardiomyocyte proliferation and did not hamper cardiomyocyte orientation and alignment. Immunostaining, ultrastructural analysis (using transmission electron microscopy), RT-PCR, pharmacological, and confocal laser calcium imaging studies demonstrated the presence of cardiac-specific molecular, ultrastructural, and functional properties of the generated tissue constructs with synchronous activity mediated by action potential propagation through gap junctions. In summary, this is the first report of the construction of 3D vascularized human cardiac tissue that may have unique applications for studies of cardiac development, function, and tissue replacement therapy.
Comparaison de diverses méthodes d’évaluation de la saturation et de la solubilité du phosphore dans des sols de Bretagne (France) à des fins agroenvironnementales. (2007)

Comparaison de diverses methodes d'evaluation de la saturation et de la solubilite du phosphore dans les sols de Bretagne (France) a des fins agroenvironnementales
Proprotein convertase subtilisin/kexin type 9 potentially influences cholesterol uptake in macrophages and reverse cholesterol transport (2013)

Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes the degradation of low-density lipoprotein receptor (LDLRs) molecules expressed on the cell surface. Gene inactivation of PCSK9 reduces the areas of atherosclerotic lesions in mice, and the effect is mainly dependent on LDLRs. Furthermore, a positive relationship between PCSK9 and cholesterol accumulation in the wall of the aorta has been established. However, the mechanism remains unknown. As PCSK9 is mainly expressed in atherosclerotic plaque and in the liver, we hypothesize that PCSK9 might increase oxidized LDL uptake and impair macrophage-mediated reverse cholesterol transport, contributing to the development of atherosclerosis.