gebhart/patrepeval huggingface dataset:
Reevaluation of the incidence of thromboembolic complications in congenital factor XII deficiency--a study on 73 subjects from 14 Swiss families. (1999)

To further elucidate the debated role of hereditary FXII deficiency as a thrombophilic risk factor this follow-up study on 65 subjects out of 12 Swiss families was undertaken (follow-up: 6 yrs). Fifteen severely FXII deficient subjects (FXII:C < 1%), 35 partially FXII deficient subjects (FXII:C > or = 1-59%), 10 with normal FXII values (FXII:C > or = 70%), and 5 non-classifiable subjects (FXII:C > or = 60-69%) were reevaluated. Eight subjects (4 severely and 3 partially FXII deficient, 1 non-classifiable) were newly enrolled. Four instances of deep vein thrombosis, one superficial vein thrombosis and one myocardial infarction were noted in 2 out of 19 severely FXII deficient subjects during a total life-time period of 866.6 patient-years. In 38 partially FXII deficient subjects (1862.8 patient-years) one ischemic cerebrovascular stroke and one superficial vein thrombosis were recorded in 2 individuals. The 10 subjects with normal FXII values (498.2 patient-years) remained thrombosis-free. One superficial vein thrombosis occurred in an unclassifiable woman. None of the 3 different FXII gene defects revealed in our patients was specifically associated with thromboembolic complications. Kaplan-Meier analysis of thrombosis-free survival suggests that hereditary partial (and probably severe) FXII deficiency does not constitute a thrombophilic condition.
MDR 1 activation is the predominant resistance mechanism selected by vinblastine in MES-SA cells (2000)

Single-step selection with vinblastine was performed in populations of the human sarcoma cell line MES-SA, to assess cellular mechanisms of resistance to the drug and mutation rates via fluctuation analysis. At a stringent selection with 20 nM vinblastine, resulting in 5-6 logs of cell killing, the mutation rate was 7 x 10(-7)per cell generation. Analysis of variance supported the hypothesis of spontaneous mutations conferring vinblastine resistance, rather than induction of adaptive response elements. Surviving clones displayed a stable multidrug resistance phenotype over a 3-month period. All propagated clones demonstrated high levels of resistance to vinblastine and paclitaxel, and lower cross-resistance to doxorubicin and etoposide. Activation of MDR 1 gene expression and P-glycoprotein function was demonstrable in all clones. No elevation was found in the expression of the mrp gene, the LRP-56 major vault protein and beta-tubulin isotypes (M40, beta4, 5beta, and beta9) in these mutants. We conclude that initial-step resistant mechanism in these vinblastine-selected mutants commonly arises from a stochastic mutation event with activation of the MDR 1 gene.
Preclinical testing of an ATR inhibitor demonstrates improved response to standard therapies for esophageal cancer (2016)

VX-970 is an effective chemo/radiosensitizer which could be readily integrated in the current treatment paradigm to improve the treatment response in esophageal cancer and we plan to test it prospectively in the forthcoming phase I dose escalation safety study combining the ATR inhibitor VX-970 with chemoradiotherapy in esophageal cancer (EudraCT number: 2015-003965-27).
Bone healing response to an injectable calcium phosphate cement with enhanced radiopacity (2007)

The aim of this study was to determine the impact of barium sulfate on remodeling and regeneration in standard tibial defects in rabbits treated with the Norian skeletal repair system (SRS). Two formulations of SRS (with and without barium sulfate) were injected into the medullary canal of the tibia of New Zealand white rabbits. Animals were sacrificed at 6 weeks, 6 months, 1 year, and 2 years. Over the 2-year duration of the study, standard SRS and SRS with barium sulfate appeared to be biocompatible and osteoconductive with no evidence of either inflammation or fibrous tissue around the implant materials or at the bone-material interfaces. This outcome underscores the osteophilic property of the SRS. A difference we observed between the standard SRS and the SRS with barium sulfate was the appearance of acellular material contiguous to the SRS with barium sulfate. Energy dispersive X-ray spectroscopy (EDX) analysis was conducted and confirmed that the acellular material was barium sulfate. Pathological examination of additional tissues including regional lymph nodes revealed neither dissemination of calcium phosphate nor barium sulfate. We concluded that the residual barium sulfate detected by EDX was localized to the intramedullary canal of the tibia.
Statistical Methodology: VI. Mathematical Modeling of the Electrocardiogram Using Factor Analysis (1998)

The 12-lead ECG can be derived from only 3 measured leads and graphed as a 3-D spatial ECG. This type of data processing may lead to instantaneous acquisition and may enhance the diagnostic capability of the ECG from routine bedside telemetry equipment.
Building Java Enterprise Systems with J2EE with CD Rom (2000)

From the Publisher:
Building Java Enterprise Systems with J2EE provides a comprehensive and practical guide for professional Java developers and architects who need to build distributed, scalable, secure, and web-enabled Enterprise, E-commerce, and Business-to-Business (B2B) systems.
The practical angle of Building Java Enterprise Systems with J2EE provides the conceptual background and wealth of code examples needed to actually assemble systems in a useful manner with the J2EE technologies. Furthermore, this book demonstrates how the technologies complement and build on top of one another via evolution of a cohesive and real sample application. You can use this book to learn, develop, and design your custom applications immediately.
Topics covered include: J2EE, EJB, servlets, JSP, XML, JMS, JDBC, Security, JNDI, Jini, CORBA, RMI, DCOM, JTA, JTS, and JavaMail.
Adaptive regression and model selection in data mining problems (1999)

Data Mining is a new and rapidly evolving area which deals with problems related to extracting structure from massive commercial and scientific data sets. Regression analysis is one of the major Data Mining techniques. The data sets encountered in the Data Mining area are often characterized by a large number of attributes (variables) as well as data records.This imposes two major requirements on the regression analysis tools used in Data Mining: first, in order to produce accurate and parsimonious models exhibiting the most important features of the problem in hand, they should be able to perform model selection adaptively and, second, the cost of running such tools has to be reasonably low. Most of the modern regression tools fail to meet the above requirements. This thesis is intended to contribute to the improvement of the existing methodologies as well as to propose new approaches. We focus on two regression estimation techniques. The first one,called Probing Least Absolute Squares Modelling (PLASM), is a generalization of the Least Absolute Shrinkage and Selection Operator (LASSO) by R. Tibshirani which minimizes the residual sum of squares subject to the 11-norm of the regression coefficients being less than a constant. LASSO has been shown to enjoy stability of the ridge regression coupled with the ability to carry out model selection. In our approach called PLASM, we replace the constraint employed in LASSO with a different constraint. PLASM allows for an arbitrary grouping of basis functions in a model and includes LASSO as a special case.The implication of using the new constraint is that PLASM is able to perform model selection in terms of groups of basis functions. This turns out to be very useful in a number of data analytic problems.For example, as far as additive modelling is concerned,the dimensionality of the PLASM minimization problem is much less than that of LASSO and is independent (at least explicitly) of the number of datapoints which makes it suitable for use in the Data Mining context. The second tool we consider this thesis is the Multivariate Adaptive Regression Splines (MARS) developed by J.Friedman.In our version of MARS called BMARS, we use B-splines instead of truncated power basis functions. The fact that B-splines have the compact support property allows us to introduce a new strategy whereby at any momen the algorithm builds a model using B-splines of a certain scale only and it switches over to splines of smaller scale after the fitting ability of the current splines has been exhausted. Also, we discuss a parallel version of BMARS as well as an application of the algorithm to processing of a large commercial data set.The results of the numerical experiments demonstrate that, while being considerably more efficient, BMARS is able to produce models competitive with those of the original MARS.
Global satellite communication network using double-layered inclined-orbit constellation with optical intersatellite links (1996)

A double-layered constellation for a future global satellite communications network connected by optical intersatellite links has been proposed. The constellation consists of lower layer satellites for mobile and personal satellite communications, and upper layer satellites for large- capacity fixed satellite communications and feeder links. Optical inter-satellite links, which can perform high-capacity communications with small terminals, are used for all intersatellite data transmission. Although a polar orbit constellation offers the merit of simpleness in network configurations, the inclined orbit constellation offers the potential for reducing the required number of satellites, improving link properties, and enhancing the coverage in middle and low latitudes, by selecting the most adequate inclination of the orbits. The optical inter- satellite link properties, coverage properties, and required number of satellites are evaluated for constellations using inclined orbits, and then compared with those of a polar orbit constellation. Three constellation types in each layer achieving continuous double coverage are assumed. For each constellation, the relations between these properties and the inclination of the orbits are examined. The basic parameters of optical inter-satellite links on satellite constellations using inclined orbits are also shown.
Alteration of anal sphincter morphology following vaginal delivery revealed by multiplanar anal endosonography (2002)

Anal sphincter morphology changes after an otherwise atraumatic vaginal delivery. This change does not correlate with any functional symptoms.
Architecture design for deblocking filter in H.264/JVT/AVC (2003)

This paper presents an efficient VLSI architecture for the deblocking filter in H.264/JVT/AVC. We use an array of 8/spl times/4 8-bit shift registers with reconfigurable data path to support both horizontal filtering and vertical filtering on the same circuit (a parallel-in parallel-out reconfigurable FIR filter). Two SRAM modules are carefully organized not only for the storage of current macroblock data and adjacent block data but also for the efficient access of pixels in different blocks. Simulation results show that under 0.25 /spl mu/m technology, the synthesized logic gate count is only 19.1 K (not including a 96/spl times/32 SRAM and a 64/spl times/32 SRAM) when the maximum frequency is 100 MHz. Our architecture design can easily support real-time deblocking of 720p (1280/spl times/720) 30 Hz video. It is valuable for platform-based design of H.264 codec.
Synergy between growth factors and transmitters required for catecholamine differentiation in brain neurons (1995)

The phenotypically plastic neurons of the embryonic mouse striatum were used to explore mechanisms of catecholamine differentiation in culture. De novo transcription and translation of the CA biosynthetic enzyme, tyrosine hydroxylase (TH), was induced in striatal neurons exposed, simultaneously or sequentially, to the growth factor, acidic fibroblast growth factor (aFGF) and a catecholamine. Although dopamine was the most potent aFGF partner (ED50 = 4 microM), a number of substances, including dopamine (D1) receptor agonists, beta-adrenoceptor agonists, and dopamine uptake inhibitors also trigger TH induction when accompanied by aFGF. However, since none of the receptor antagonists nor transport blockers tested could inhibit dopamine's action, the mechanism remains obscure. Structure-activity analysis suggests that effective aFGF partners all contain an amine group separated from a catechol nucleus by two carbons. Thus, TH expression can be novelly induced by the synergistic interaction of aFGF, and to a lesser extent basic FGF, and a variety of CA-containing partner molecules. We speculate that a similar association between growth factor and transmitter may be required in development for the differentiation of a CA phenotype in brain neurons.
DNA polymerase III accessory proteins. V. Theta encoded by holE (1993)

The polIII core subassembly of DNA polymerase III holoenzyme is composed of the alpha (DNA polymerase), epsilon (editing exonuclease), and theta subunits. We have identified holE encoding theta (8.6 kDa) at 40.4 min, expressed and purified 300 mg of theta, and have studied its function by constituting the polIII core from pure alpha, epsilon, and theta subunits. The theta subunit binds the epsilon proofreader tightly, but it does not form a detectable complex with alpha. The epsilon subunit also binds to alpha (Maki, H., and Kornberg, A. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 4389-4392). Hence, the subunit arrangement of the polIII core is linear, alpha epsilon theta. Interaction of theta with epsilon slightly stimulated epsilon in excision of a 3' terminal mismatched nucleotide, suggesting a possible role for theta in fidelity.
A Bifunctional Poly(ethylene glycol) Silane Immobilized on Metallic Oxide-Based Nanoparticles for Conjugation with Cell Targeting Agents (2004)

A trifluoroethylester-terminal poly(ethylene glycol) (PEG) silane was synthesized and self-assembled on iron oxide nanoparticles. The nanoparticle system thus prepared has the flexibility to conjugate with cell targeting agents via either carboxylic or amine terminal groups for a number of biomedical applications, including magnetic resonance imaging (MRI) and controlled drug delivery. The trifluoroethylester silane was synthesized by modifying a PEG diacid to form the corresponding bistrifluoroethylester (TFEE), followed by a reaction with 3-aminopropyltriethoxysilane (APS). The APS coupled with PEG chains confers the stability of PEG self-assembled monolayers (SAMs) and increases the PEG packing density on nanoparticles by establishing hydrogen bonding between the carbonyl and amine groups present within the monolayer structure. The success of the synthesis of the PEG TEFE silane was confirmed with (1)H NMR and Fourier transform infrared spectroscopy (FTIR). The conjugating flexibility of the PEG TEFE was demonstrated with folic acid that had carboxylic acid groups and amine terminal groups, respectively, and was confirmed by FTIR. TEM analysis showed the well-dispersed nanoparticles before and after they were coated with PEG and folic acid.
Hybrid Organic−Inorganic Nanocomposites:  Exfoliation of Magadiite Nanolayers in an Elastomeric Epoxy Polymer (1998)

A newly developed class of paraffin-like organomagadiite intercalates, interlayered by primary, secondary, tertiary, and quaternary onium ions, has been used to form elastomeric polymer-layered silicate nanocomposites by in situ polymerization during the thermoset process. Depending on the nature of the onium ions, intercalated or exfoliated magadiite nanocomposites were obtained. The exfoliated nanocomposites were typically disordered, but a new type of exfoliated structure also was observed in which the nanolayers were regularly spaced over long distances (e.g., ∼80 Å Bragg spacings). The tensile properties of the polymer matrix were improved greatly by the reinforcement effect of the silicate nanolayers. Exfoliated silicate nanolayers were more effective than intercalated assemblies of nanolayers in optimizing reinforcement. Interestingly, organomagadiite exfoliation in the rubbery epoxy matrix improves the elongation-at-break while improving tensile strength, which is opposite to the behavior of conventional composites. The tensile properties of the new epoxy−magadiite elastomeric nanocomposites are compared with those for previously reported nanocomposites derived from smectite clays.
Post‐transcriptional regulation of gene expression by degradation of messenger RNAs (2003)

Recent evidence suggests that gene expression may be regulated, at least in part, at post-transcriptional level by factors inducing the extremely rapid degradation of messenger RNAs. These factors include reactions between adenyl-uridyl-rich elements (AREs) of the relevant mRNA and either specific proteins that bind to these elements or exosomes. This review deals with examples of the proteins (AU-rich binding proteins, AUBPs) and exosomes, which have been shown to form complexes with AREs and bring about rapid degradation of the relevant mRNA, and with certain other factors, which protect the RNA from such degradation. The biochemical and physiological factors underlying the stability of messenger RNAs carrying the ARE motifs will be reviewed in the light of their emerging significance for cell physiology, human pathology, and molecular medicine. We also consider the possible application of the results of recent insights into the mechanisms to pharmacological interventions to prevent or cure disorders, especially developmental disorders, which the suppression of gene expression may bring about. Molecular targeting of specific steps in protein degradation by synthetic compounds has already been utilized for the development of pharmacological therapies.
The effect of oxidant injury on the lymphocyte membrane and functions. (1981)

We have studied the effect of oxidant stress on the lymphocyte membrane and lymphocyte functions. Lymphocyte cultures were incubated with xanthine oxidase and xanthine, an enzyme system known to generate several highly reactive oxygen compounds. We demonstrated that these lymphocytes were viable after exposure to an in vitro oxidant stress. However, there was a marked reduction in their ability to bind SRBCs and to form caps after Con A stimulation. These lymphocytes also demonstrated a delay in PHA-induced LBT, with maximal response occurring at 5 days instead of 3 days. Catalase, a hydrogen peroxide scavenger, protected lymphocytes from this injury, implicating hydrogen peroxide as the causative agent. Another lymphocyte membrane-related function, the ability to stimulate or respond in MLC, was not impaired after oxidant injury. These results demonstrate that after in vitro oxidant injury, lymphocytes may have alterations in the cell membrane and impaired function.
Granulocyte colony-stimulating factor enhances alpha-naphthylthiourea-induced pulmonary hypertension (2003)

Physiopathological discrepancies exist between the most widely used models of pulmonary hypertension (PH), namely monocrotaline- and hypoxia-induced PH. The development of a new model could help in the understanding of underlying mechanisms. Repeated alpha-naphthylthiourea (ANTU) injections (5 mg/kg weekly, 3 wk) induced pulmonary vascular remodeling, which was associated with development of PH and right ventricular hypertrophy. ANTU followed by granulocyte colony-stimulating factor (G-CSF; 25 microgram. kg(-1). day(-1) subcutaneously, 3 days/wk) induced higher pulmonary arterial pressures and right ventricular hypertrophy than ANTU alone. Lidocaine, which inhibits neutrophil functions, inhibited PH exacerbation by G-CSF. Endothelial nitric oxide synthase expression, measured to assess ANTU-related endothelial toxicity, decreased significantly in ANTU-treated rats and fell even more sharply when G-CSF was given. This occurred despite a significant increase in vascular endothelial cell growth factor expression in lung and right ventricle in rats given ANTU alone and even more in rats given ANTU plus G-CSF. Repeated ANTU administration induces PH with vascular remodeling that can be further aggravated by the neutrophil activator G-CSF.
α-Glucosidase Mutant Catalyzes “α-Glycosynthase”-type Reaction (2002)

Replacement of the catalytic nucleophile Asp481 by glycine in Schizosaccharomyces pombe alpha-glucosidase eliminated the hydrolytic activity. The mutant enzyme (D481G) was found to catalyze the formation of an alpha-glucosidic linkage from beta-glucosyl fluoride and 4-nitrophenyl (PNP) alpha-glucoside to produce two kinds of PNP alpha-diglucosides, alpha-isomaltoside and alpha-maltoside. The two products were not hydrolyzed by D481G, giving 41 and 29% yields of PNP alpha-isomaltoside and alpha-maltoside, respectively. PNP monoglycosides, such as alpha-xyloside, alpha-mannoside, or beta-glucoside, acted as the substrate, but PNP alpha-galactoside and maltose could not. No detectable product was observed in the combination of alpha-glucosyl fluoride and PNP alpha-glucoside. This study is the first report on an "alpha-glycosynthase"-type reaction to form an alpha-glycosidic linkage.
The prolonged intake of L-arginine-L-aspartate reduces blood lactate accumulation and oxygen consumption during submaximal exercise. (2005)

L-arginine-L-aspartate is widely used by athletes for its potentially ergogenic properties. However, only little information on its real efficacy is available from controlled studies. Therefore, we evaluated the effects of prolonged supplementation with L-arginine-L-aspartate on metabolic and cardiorespiratory responses to submaximal exercise in healthy athletes by a double blind placebo-controlled trial. Sixteen healthy male volunteers (22 ± 3 years) performed incremental cycle spiroergometry up to 150 watts before and after intake of L-arginine-L-aspartate (3 grams per day) or placebo for a period of 3 weeks. After intake of L-arginine-L-aspartate, blood lactate at 150 watts dropped from 2.8 ± 0.8 to 2.0 ± 0.9 mmol·l(-1) (p < 0.001) and total oxygen consumption during the 3-min period at 150 watts from 6.32 ± 0.51 to 5.95 ± 0.40 l (p = 0.04) compared to placebo (2.7 ± 1.1 to 2.7 ± 1.4 mmol·l(-1); p = 0.9 and 6.07 ± 0.51 to 5.91 ± 0.50 l; p = 0.3). Additionally, L-arginine-L-aspartate supplementation effected an increased fat utilisation at 50 watts. L-arginine and L-aspartate seem to have induced synergistic metabolic effects. L-arginine might have reduced lactic acid production by the inhibition of glycolysis and L-aspartate may have favoured fatty acid oxidation. Besides, the results indicate improved work efficiency after L-arginine-L-aspartate intake. The resulting increases of submaximal work capacity and exercise tolerance may have important implications for athletes as well as patients. Key PointsAmino acids are among the most common nutritional supplements taken by athletes. They are involved in numerous metabolic pathways that affect exercise metabolism.Three weeks of L-arginine-L-aspartate supplementation resulted in lower blood lactate concentrations and oxygen consumption, diminished glucose and enhanced fat oxidation, and reduced heart rate and ventilation during submaximal cycle exercise.This implies increased submaximal work capacity and exercise tolerance, which may have important implications for both athletes as well as patients.