gebhart/patrepeval huggingface dataset:
SSZ‐53 and SSZ‐59: Two Novel Extra‐Large Pore Zeolites (2003)

The syntheses, structure solutions, and physicochemical and catalytic characterizations of the novel zeolites SSZ-53 and SSZ-59 are described. SSZ-53 and SSZ-59 were synthesized under hydrothermal conditions with the [1-(4-fluorophenyl)cyclopentylmethyl]trimethyl ammonium cation and 1-[1-(4-chlorophenyl)cyclopentylmethyl]-1-methyl azocanium cation, respectively, as structure-directing agents. The framework topology of SSZ-53 was solved with the FOCUS method, and the structure of SSZ-59 was determined by model building. Rietveld refinement of synchrotron X-ray powder diffraction data confirms each proposed model. SSZ-53 and SSZ-59 each possess a one-dimensional channel system delimited by 14-membered rings. Results from transmission electron microscopy, electron diffraction, catalytic experiments (spaciousness index and constraint index tests), and argon and hydrocarbon adsorption experiments are consistent with the proposed structures.
Cross-Species Antiviral and Antiproliferative Activity of Human Interferon-ω (1994)

The antiviral and antiproliferative activities of human interferon-omega (IFN-omega) on two human cell lines and on VERO (monkey), MDBK (calf), SPEV (pig), L929 (mouse), BHK-21 (hamster), and MDCK (dog) cell lines were compared with those of human IFN-alpha 1 and IFN-alpha 2. The results are tabulated. Compared with its antiviral titer on human A549 cells, INF-omega was more active on mouse cells and even more active on the pig cells, but had little activity on the hamster cells and virtually none on the dog cells. IFN-omega also inhibited the growth of all these cells to a greater or lesser extent, and there was in general an apparent correlation between its antiviral and antiproliferative activities on the different cells, except that the dog cells were relatively much more sensitive to the antiproliferative effect.
A Connection between the Mitochondrial Permeability Transition Pore, Autophagy, and Cerebral Amyloidogenesis (2008)

In a drug reprofiling attempt, we explored novel neuroprotective properties of 4-azasteroids by synthesizing chemical affinity tags capturing adenine nucleotide translocator-1, as a potential target. Dutasteride inhibits the mitochondrial transition pore and induces an increase of autophagosomal structures in human cell lines. In vivo, a surprising reduction of the beta-amyloid plaque load in a model for cerebral amyloidosis appears to connect release of neurotoxic peptides, mitochondrial apoptosis and autophagy.
Using light as a lens for submicron, neutral-atom lithography (1992)

We show that light can be used as a lens to focus a collimated neutral atomic beam to submicron dimensions during deposition onto a substrate. We have used an optical standing wave at 589 nm as an array of cylindrical lenses to focus a perpendicular sodium beam into a grating on a substrate, with a periodicity of 294.3\ifmmode\pm\else\textpm\fi{}0.3 nm. This result is the first direct evidence of submicron focusing of atoms by light, and represents a fundamentally new scheme for submicron lithography.
Crop Rotation and Residue Management Effects on Soil Carbon and Microbial Dynamics (1992)

Abstract Understanding microbial dynamics is important in the development of new management strategies to reverse declining organic‐matter content and fertility of agricultural soils. To determine the effects of crop rotation, crop residue management, and N fertilization, we measured changes in microbial biomass C and N and populations of several soil microbial groups in long‐term (58‐yr) plots under different winter wheat ( Triticum aestivum L.) crop rotations. Wheat‐fallow treatments included: wheat straw incorporated (5 Mg ha −1 ), no N fertilization; wheat straw incorporated, 90 kg N ha −1 ; wheat straw fall burned, no N fertilization; and wheat straw incorporated, 11 Mg barnyard manure ha −1 . Annual‐crop treatments were: continuous wheat, straw incorporated, 90 kg N ha −1 ; wheat‐pea ( Pisum sativum L.) rotation (25 yr), wheat and pea straw incorporated, 90 kg N ha −1 applied to wheat; and continuous grass pasture. Total soil and microbial biomass C and N contents were significantly greater in annual‐crop than wheat‐fallow rotations, except when manure was applied. Microbial biomass C in annual‐crop and wheat‐fallow rotations averaged 50 and 25%, respectively, of that in grass pasture. Residue management significantly influenced the level of microbial biomass C; for example, burning residues reduced microbial biomass to 57% of that in plots receiving barnyard manure. Microbial C represented 4.3, 2.8, and 2.2% and microbial N 5.3, 4.9, and 3.3% of total soil C and N under grass pasture, annual cropping, and wheat‐fallow, respectively. Both microbial counts and microbial biomass were higher in early spring than other seasons. Annual cropping significantly reduced declines in soil organic matter and soil microbial biomass.
The use of biological assays for detection of polypeptide growth factors (1990)

Polypeptide growth factors can be identified and quantified with high accuracy by the use of specific biological assays. In general these bioassays are highly sensitive for detection of growth factor activity, and enhanced specificity can be obtained by a proper choice of selective culture conditions for the target cells involved. In this paper sensitive and selective bioassays are described for growth factors acting on substrate-attached cells, in particular members of the epidermal growth factor, transforming growth factor beta, platelet-derived growth factor, insulin-like growth factor, and heparin-binding growth factor families. A cross-reactivity scheme has been worked out to identify possible contaminations in growth factor preparations.
A 19 W RGB solid-state laser source for large frame laser projection displays (2002)

A diode-pumped all-solid-state laser system provides simultaneously red (R), green (G) and blue (B) laser light with output powers of 7.1 W at 629 nm, 6.9 W at 532 nm and 5.0 W at 446 nm. The superposition of the RGB radiation generates white light with a power of 19 W. The RGB output corresponds to an optical efficiency of 17% regarding the system's total diode pump power of 110 W. The system is based on a mode-locked Nd:YVO/sub 4/ laser system, and a synchronously pumped near IR optical parametric oscillator.
Ubiquitous high-level gene expression in hematopoietic lineages provides effective lentiviral gene therapy of murine Wiskott-Aldrich syndrome (2012)

The immunodeficiency disorder Wiskott-Aldrich syndrome (WAS) leads to life-threatening hematopoietic cell dysfunction. We used WAS protein (WASp)-deficient mice to analyze the in vivo efficacy of lentiviral (LV) vectors using either a viral-derived promoter, MND, or the human proximal WAS promoter (WS1.6) for human WASp expression. Transplantation of stem cells transduced with MND-huWASp LV resulted in sustained, endogenous levels of WASp in all hematopoietic lineages, progressive selection for WASp+ T, natural killer T and B cells, rescue of T-cell proliferation and cytokine production, and substantial restoration of marginal zone (MZ) B cells. In contrast, WS1.6-huWASp LV recipients exhibited subendogenous WASp expression in all cell types with only partial selection of WASp+ T cells and limited correction in MZ B-cell numbers. In parallel, WS1.6-huWASp LV recipients exhibited an altered B-cell compartment, including higher numbers of λ-light-chain+ naive B cells, development of self-reactive CD11c+FAS+ B cells, and evidence for spontaneous germinal center (GC) responses. These observations correlated with B-cell hyperactivity and increased titers of immunoglobulin (Ig)G2c autoantibodies, suggesting that partial gene correction may predispose toward autoimmunity. Our findings identify the advantages and disadvantages associated with each vector and suggest further clinical development of the MND-huWASp LV for a future clinical trial for WAS.
Chronic Stage Multiple Sclerosis Lesions Contain a Relatively Quiescent Population of Oligodendrocyte Precursor Cells (1998)

In the past decade, considerable progress has been made in the understanding of the biology of rodent oligodendrocyte precursor cells and their role in the generation of oligodendrocytes in the developing and adult rodent CNS. Much less is known about human oligodendrocyte lineage cells and about the reasons for the failure of the regeneration of the oligodendrocyte population during chronic stages of multiple sclerosis (MS). In particular, the fate of the oligodendrocyte precursor population in MS has remained elusive. The present study examined the possibility that oligodendrocyte regeneration ultimately fails because of the local destruction of both oligodendrocytes and their precursor cells. Analysis of chronic stage MS tissue suggested that this is not the case, because all chronic MS lesions studied contained significant numbers of oligodendrocyte precursor cells, identified as process-bearing cells that bound the O4 antibody but not antibodies to GalC and GFAP. The oligodendrocyte precursor cells appeared, however, to be relatively quiescent, because none expressed the nuclear proliferation antigen recognized by the Ki-67 antibody, and because most lesions lacked myelinating oligodendrocytes in their centers. Thus, it appears that the regeneration of the oligodendrocyte population fails during chronic stages of MS because of the inability of oligodendrocyte precursor cells to proliferate and differentiate rather than because of the local destruction of all oligodendrocyte lineage cells. The identification of ways of stimulating the endogenous oligodendrocyte precursor population to expand and generate remyelinating cells may represent an alternative to transplantation of oligodendrocyte lineage cells to promote myelin repair in MS.
Presence of E6 and E7 mRNA from Human Papillomavirus Types 16, 18, 31, 33, and 45 in the Majority of Cervical Carcinomas (2006)

The oncogenic potential of the human papillomavirus (HPV) early genes E6 and E7 is well established and a source of interest with regard to HPV testing for cervical carcinoma. Here we present a study performed with 204 histologically confirmed invasive cervical squamous cell carcinomas (SCCs) in which we evaluated the HPV E6 and E7 mRNA detection assay PreTect HPV-Proofer for detection of high-risk HPV types 16, 18, 31, 33, and 45. For further evaluation, detection of E6 and E7 mRNA from HPV types 35, 52, and 58 by real-time multiplex nucleic acid sequence-based amplification was also included. For comparison and to assess the overall prevalence of various HPV types, samples were also tested for HPV DNA by both consensus and type-specific PCR, reverse line blotting, sequencing, and in situ hybridization. The overall prevalence of HPV was 97%. HPV E6 and E7 transcripts were detected in 188 of 204 (92%) biopsy specimens, of which 181 contained one of the following HPV types: 16, 18, 31, 33, or 45. Consensus PCR and type-specific PCR detected HPV in 187 of 204 and 188 of 204 (92%) specimens, respectively. In conclusion, this study verifies the presence of HPV E6 and E7 mRNA in SCCs and demonstrates that HPV infections among Norwegian women with SCCs are limited mainly to the five high-risk types, 16, 18, 31, 33, and 45. This, together with the fact that PreTect HPV-Proofer detects the HPV oncogenic transcripts, suggests that the assay is a valuable approach in the field of HPV detection in cervical carcinoma.
Investigation of selective JAK1 inhibitor GSK2586184 for the treatment of psoriasis in a randomized placebo-controlled phase IIa study (2016)

Our study demonstrates that 12 weeks of treatment with GSK2586184 resulted in clinical improvement and was generally well tolerated in patients with moderate-to-severe plaque-type psoriasis.
Hybrid modular sensor systems: a new generation of electronic noses (2002)

We present a new very flexible design of a sensor system for gas and odor analysis, which may easily be optimized for specific applications. The core part contains a digital bus system that connects individual sensor modules with a central processor. By using dedicated microcontrollers onboard the modules it is possible to use any kind of transduction principles ("hybrld system"), with typical numbers of individual sensor elements for each transducer in the order of 8. The measured sensor parameters are transmitted via a standard protocol on the digital bus system. Hence all types of modules are interchangeable. This allows an easy adaption of new technologies to new applications. The specifications of the interface are available to developers and researchers, thus offering them the possibility to introduce their own specific technologies for certain applications. The system allows combination of self-made sensors with commercial ones and testing of a growing range of other sensor technologies. It can thus be used as a benchmarking tool.
On the minimal synchronism needed for distributed consensus (1987)

Reaching agreement is a primitive of distributed computing. Whereas this poses no problem in an ideal, failure-free environment, it imposes certain constraints on the capabilities of an actual system: A system is viable only if it permits the existence of consensus protocols tolerant to some number of failures. Fischer et al. have shown that in a completely asynchronous model, even one failure cannot be tolerated. In this paper their work is extended: Several critical system parameters, including various synchrony conditions, are identified and how varying these affects the number of faults that can be tolerated is examined. The proofs expose general heuristic principles that explain why consensus is possible in certain models but not possible in others.
REFLECTION AND TRANSMISSION SECONDARY EMISSION FROM SILICON (1970)

Reflection and transmission secondary emission ratios have been measured for a thin Si sample with one surface activated to a state of negative electron affinity. Reflection-mode gains of 950 were observed at 20-keV primary energy. The highest transmission secondary emission ratio was 725 at 20-keV primary energy. The high secondary emission gains result from the effective negative electron affinity at the surface of the sample together with a long escape depth for internal secondaries.
Prediction of the Coding Sequences of Unidentified Human Genes. XIII. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro (1999)

As a part of our cDNA project for deducing the coding sequence of unidentified human genes, we newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0919 to KIAA1018. The sequencing of these clones revealed that the average sizes of the inserts and corresponding open reading frames were 4.9 kb and 2.6 kb (882 amino acid residues), respectively. A computer search of the sequences against the public databases indicated that predicted coding sequences of 87 genes contained sequences similar to known genes, 53% of which (46 genes) were categorized as proteins relating to cell signaling/communication, cell structure/motility and nucleic acid management. The chromosomal locations of the genes were determined by using human-rodent hybrid panels unless their mapping data were already available in the public databases. The expression profiles of all the genes among 10 human tissues, 8 brain regions (amygdala, corpus callosum, cerebellum, caudate nucleus, hippocampus, substania nigra, subthalamic nucleus, and thalamus), spinal cord, fetal brain and fetal liver were also examined by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.
Effects of Enhancing Slow-Wave Sleep by Gamma-Hydroxybutyrate on Obstructive Sleep Apnea (1992)

Sleep apneas are rarely observed during slow-wave sleep (SWS), which is poorly represented in patients with obstructive sleep apnea (OSA). Gamma-hydroxybutyrate (gamma OH), a natural metabolite of the brainstem, increases the percentage of total sleep time (TST) spent in SWS. We evaluated the effects of gamma OH on sleep and breathing disorders in eight patients with OSA (age, 45 +/- 2 yr; body mass index, 35.0 +/- 1.5 kg/m2; mean +/- SEM). Three conventional sleep studies were done within a week; the first and third were control studies, and the second was the gamma OH study (30 mg/kg at bedtime and 3 h later). Because the effects of the drug last only 3 h, we analyzed and compared the results of the first 6 h of sleep recording. The percentage of TST spent in SWS increased with gamma OH (30.7 +/- 3.9%) compared with that in the control studies (12.5 +/- 1.1 and 11.0 +/- 2.1%) at the expense of stages I and II. There was no difference between apnea index obtained during the control studies (26.3 +/- 5.3 and 25.4 +/- 6.2/h) and that obtained during the gamma OH study (29.6 +/- 4.9/h). Most apneic events occurred during Stages I and II, and REM, but this proportion was less during the gamma OH study (77.9 +/- 8.9%) than during the control studies (92.3 +/- 1.9 and 95.9 +/- 2.2%), apneas occurring even during SWS with gamma OH.(ABSTRACT TRUNCATED AT 250 WORDS)
Virus reduction in the preparation of intravenous immune globulin: in vitro experiments (1999)

BACKGROUND: While immune globulins for intravenous administration (IGIV) have an excellent record with respect to virus safety, concern regarding these preparations has been raised by reports of transmission of hepatitis C virus (HCV) to patients treated with IGIV and the presence of genetic material for HCV in IGIV preparations. STUDY DESIGN AND METHODS: This in vitro study evaluated the effectiveness of several manufacturing steps, including ethanol precipitation and pasteurization, in reducing HIV and model viruses including encephalomyocarditis (EMC) virus, pseudorabies virus (PRV), bovine viral diarrhea virus (BVDV), Sindbis virus, vaccinia virus, and vesicular stomatitis virus (VSV), as well as HCV RNA, in IGIV. RESULTS: Ethanol precipitation carried out after pasteurization resulted in virus reductions (log 10 ) of &gt;3.97 for HIV, 1.95 for EMC virus, &gt;5.39 for PRV, and 3.52 for BVDV. Pasteurization inactivated EMC virus by 4.52 log 10 and resulted in a log 10 reduction of &gt;6.54 for HIV, &gt;5.39 for PRV, &gt;6.64 for BVDV, &gt;7.78 for Sindbis virus, &gt;5.84 for vaccinia virus, and &gt;6.99 for VSV. All viruses except EMC virus were reduced below the limit of detection within 6 hours of the beginning of pasteurization. Cohn processing of Fraction II + III paste and the 4.5‐percent alcohol precipitation step prior to pasteurization provided additional virus removal. Studies using the polymerase chain reaction technique found that HCV RNA was detectable in the starting fraction of Cohn Fraction II paste, but not in the final IGIV preparation. CONCLUSION: These findings strongly support the viral safety of IGIV prepared by this method and show a significant added measure of virus safety associated with pasteurization of this preparation.
Human/severe combined immunodeficient mouse chimeras. An experimental in vivo model system to study the regulation of human endothelial cell-leukocyte adhesion molecules. (1993)

The ability of circulating white blood cells to enter inflamed tissues is mediated by specific cell adhesion molecules thought to be expressed in a programmed and sequential manner to form an "adhesion cascade." Because of the complexity of this process, it is becoming increasingly important to develop in vivo models. Two major problems have limited the utility of current animal models. The first is the inability of many of the antibodies developed against cell adhesion molecules in human cell culture models to cross-react in animals. The second is the uncertainty in extrapolating animal (particularly rodent) findings to humans. To circumvent these problems, full thickness human skin grafts were transplanted onto immunodeficient (severe combined immunodeficient) mice. After 4-6 wk, the transplanted skin grafts closely resembled normal skin histologically and maintained their human vasculature as determined by immunohistochemical staining with human-specific endothelial cell markers. Intradermal injection of tumor necrosis factor-alpha resulted in the reversible upregulation of the leukocyte-endothelial adhesion molecules E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1, and in an active inflammatory reaction with migration of murine leukocytes into cytokine-injected areas. These results indicate that the severe combined immunodeficient mouse/human skin transplant model provides a useful in vivo system in which to study human endothelium during the process of inflammation.
Instantaneous frequency, instantaneous bandwidth and the analysis of multicomponent signals (2002)

The definitions of instantaneous frequency are reviewed in the context of signal theory, communication theory and discrete time implementations. The spread of a signal about its instantaneous frequency, or instantaneous bandwidth, is investigated for several common time-frequency distributions by simulations. The instantaneous frequency of a multicomponent signal is examined. Further investigation of the time-frequency representation of a multicomponent signal leads to an adaptive algorithm which may improve time-frequency resolution of multicomponent signals.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Silibinin prevents amyloid β peptide‐induced memory impairment and oxidative stress in mice (2009)

Silibinin prevents memory impairment and oxidative damage induced by Abeta(25-35) and may be a potential therapeutic agent for Alzheimer's disease.
Sizes and map positions of several plasmid-DNA-encoded transcripts in octopine-type crown gall tumors. (1982)

The sizes and relative map positions of RNA molecules encoded by the plasmid DNA of several octopine-type tobacco crown gall tumors were determined by blot hybridization. At least six discrete polyadenylylated RNAs (sizes 0.73-1.75 kilobases) were detected in octopine-producing tumors (lines A6S/2, E9, and 15955/01). In one tumor (line 15955/1) in which octopine could not be detected, one of the RNA species was missing and another was diminished in abundance. Evidence is presented suggesting that the transcripts are encoded entirely by the plasmid DNA and do not originate from the surrounding plant DNA sequences.