gebhart/patrepeval huggingface dataset:
Dopamine: whatʼs new in migraine? (2010)

Dopamine is currently considered to contribute to the pathophysiology of migraine, and dopamine receptor antagonists are prescribed in the treatment of acute migraine. Laboratory data suggest that the role of dopamine in migraine is more complex, perhaps due to the multiple receptors and levels of the brain involved in the disorder. These data suggest a reappraisal of dopaminergic therapeutic targets in migraine as our understanding of the role of this important biogenic amine is better characterized.
40 Gbit/s (16 × 2.5 Gbit/s) unrepeatered transmissionover 427 km (1996)

Unrepeatered transmission at 40 Gbit/s (16 × 2.5 Gbit/s) is demonstrated over 427 km of non-dispersion shifted fibre, with remote amplification, dispersion compensation and forward error correction. The transmission distance is extended to 450 km at 20 (8 × 2.5) and 475 km at 10 Gbit/s (4 × 2.5 Gbit/s).
Analysis of Gas—Plasma-Modified Poly(dimethylsiloxane) Elastomer Surfaces (1993)

Attenuated-total-reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is a surface-sensitive technique that is particularly suitable for the analysis of plasma-treated silicone elastomers. This chapter discusses the recent advances in gas-plasma treatments of silicone elastomer surfaces and surface analysis using ATR-FTIR spectroscopy. A particular focus is given to silicone elastomer surfaces that are plasma treated with non-film-forming gases such as argon, nitrogen, carbon dioxide, oxygen, and ammonia. The non-film-forming nature of the aforementioned gases leads to surface functionalization by incorporation of such gases into the film surface, as observed for ammonia, oxygen, and carbon dioxide gases. Furthermore, silicone elastomer surfaces can be functionalized through reactions of the surface atoms, as demonstrated for argon and nitrogen.
AlGaN/GaN Schottky-ohmic combined anode field effect diode with fluoride-based plasma treatment (2008)

Proposed is a novel AlGaN/GaN field effect diode with relatively thick AlGaN barrier layer, named the Schottky-ohmic combined anode field effect diode (SOCFED) because of its distinguishing anode structure. To obtain a low turn-on voltage, a method of fluoride-based plasma exposure was used. The fabricated SOCFED has a turn-on voltage of 0 V and a breakdown voltage of over 200 V.
Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation (2000)

Morphine dehydrogenase (MDH) of Pseudomonas putida M10 catalyses the NADP+-dependent oxidation of morphine and codeine to morphinone and codeinone. This enzyme forms the basis of a sensitive detection and assay method for heroin metabolites and a biotransformation process for production of hydromorphone and hydrocodone. To improve these processes we have undertaken a thorough examination of the kinetic mechanism of MDH. Sequence comparisons indicated that MDH belongs within the aldose reductase enzyme family. MDH was shown to be specific for the pro-R hydrogen of NADPH. In steady-state kinetic studies, product inhibition patterns suggested that MDH follows a Theorell-Chance mechanism for codeinone reduction at pH 7, and a non-Theorell-Chance sequential ordered mechanism for codeine oxidation at pH 9.5. Residues corresponding to the catalytically important Tyr-48, Lys-77 and Asp-43 of aldose reductase were modified by site-directed mutagenesis, resulting in substantial loss of activity consistent with a catalytic role for these residues. Loss of activity of MDH in the presence of the reaction product morphinone was found to be due to the formation of a covalent adduct with Cys-80; alteration of Cys-80 to serine resulted in an enzyme with greatly enhanced stability.
Investigation of Different Methods to Control a Small Variable-Speed Wind Turbine With PMSM Drives (2006)

In this paper, a different control strategy of a small wind generator, including a permanent magnet synchronous machine (PMSM), has been studied. The objective is to analyze the produced power quality of small wind turbines connected to weak AC grids. The extraction of the electrical energy from the wind turbine is based on a maximum power point tracking (MPPT) algorithm to control a pulse width modulation (PWM) rectifier. The grid connection is realized by means of a PWM voltage source inverter via a filter. This inverter is controlled by three different methods based on current or active and reactive power control. These methods are applicable for different power ranges. The obtained results demonstrate the efficiency of the system and the energetic contribution, mainly for rural weak AC grids.
Many rhinovirus serotypes share the same cellular receptor (1984)

Twenty-four human rhinovirus serotypes were grown and purified by centrifugation in metrizamide density gradients. These preparations had a lower buoyant density (1.24 g/cm3) and higher specific infectivities (1:24 to 1:240) than did rhinoviruses described previously (E. J. Stott and R. J. Killington, Annu. Rev. Microbiol. 26:503-524, 1972). Binding conditions in which the unique cellular receptors for virus attachment were saturated were determined for each serotype. Competition binding assays between pairs of serotypes allowed 20 of the 24 serotypes to be assigned to the same cellular receptor. The remaining four serotypes appeared to attach to a different cellular receptor. Since most serotypes were chosen for study at random, it seems likely that many of the yet unstudied rhinoviruses will share this common cellular receptor.
Random or Rational Design? Evaluation of Diverse Compound Subsets from Chemical Structure Databases (1998)

The performance of rational design to maximize the structural diversity of databases for lead finding and lead refinement was investigated. Rational methods such as maximum dissimilarity methods or hierarchical cluster analysis for designing compound subsets were compared to a random approach to study their efficiency for an enhancement of the diversity of three different databases. All investigations were done based on 2D fingerprints as a validated molecular descriptor. To compare the performance of the rational selection methods to a random approach, we additionally used probability calculations. When using maximum dissimilarity-based selections, a single compound can be a member of different neighborhoods as defined by the similarity threshold value, while in hierarchical clustering each compound is assigned to only a single cluster. Therefore the relationship between the similarity threshold of the maximum diversity selection method and a 2D similarity search threshold was studied. In contrast to hierarchical clustering analysis, maximum dissimilarity selections allow to use a similarity threshold for adding a new compound to an already selected compound list. Reasonable values for this similarity threshold are presented here. More diverse subsets were designed using maximum dissimilarity selections, which cover more biological classes than using random selections. An optimally diverse subset without redundant structures containing only 38% of one original dataset was generated, where no structure is more similar than 0.85 to its nearest neighbor, but all biological classes were represented. When it is acceptable to cover only 90% of all biological targets, 3.5-3.7 times more compounds need to be selected using a random approach than in a rational design approach. Such coverage rate shows the highest efficiency of design techniques compared to a random approach. In those subsets no compound is closer than 0.70 to its nearest neighbor. Furthermore a comparative molecular field analysis (CoMFA) is used to evaluate designed and randomly chosen subsets for a database consisting of inhibitors of the angiotensin-converting enzyme. It was shown that designed subsets using maximum dissimilarity methods lead to more stable quantitative structure-activity relationship (QSAR) models with higher predictive power compared to randomly chosen compounds. This predictive power is especially high when there is no compound in the test dataset with a similarity coefficient less than 0.7 to its nearest neighbor in the training set.
IL-15–PI3K–AKT–mTOR: A Critical Pathway in the Life Journey of Natural Killer Cells (2015)

Among numerous cytokines modulating natural killer (NK) cell function, interleukin 15 (IL-15) exerts a broad range of effect from development and homeostasis, to activation of mature NK cells during infection. Its significance is further highlighted by clinical trials in which IL-15 is being used to boost the proliferation and anti-tumor response of NK cells. Among the signal transduction pathways triggered by the engagement of IL-15 receptor with its ligand, the PI3K-AKT-mTOR pathway seems to be critical for the IL-15-mediated activation of NK cells, therefore being responsible for efficient anti-viral and anti-tumor responses. This review provides an overview of the role of IL-15 at multiple stages of NK cell life journey. Understanding the pathway by which IL-15 conveys critical signals for the generation of NK cells with efficient effector functions, in combination with established protocols for NK cell expansion ex vivo, will undoubtedly open new avenues for therapeutic applications for immunomodulation against infections and cancers.
Purinergic cotransmission (2008)

Adenosine 5'-triphosphate (ATP) is a cotransmitter with classical transmitters in most nerves in the peripheral and central nervous systems, although the proportions vary between tissues and species and in different developmental and pathophysiological circumstances. There was early evidence that ATP was released together with acetylcholine (ACh) from motor nerves supplying skeletal muscle, although it was considered at the time as a molecule involved in the vesicular uptake and storage of ACh. Later it was shown that in the developing neuromuscular junction, released ATP acted on P2X receptor ion channels as a genuine cotransmitter with ACh. Adenosine triphosphate was shown to be released from sympathetic nerves supplying the guinea-pig taenia coli in 1971. Soon after, the possibility was raised that ATP was coreleased with noradrenaline from sympathetic nerves to guinea-pig seminal vesicle, cat nictitating membrane and guinea-pig vas deferens. Sympathetic purinergic cotransmission has also been demonstrated in many blood vessels. Parasympathetic nerves supplying the urinary bladder use ACh and ATP as cotransmitters; ATP acts through P2X ionotropic receptors, whereas the slower component of the response is mediated by the metabotropic muscarinic receptor. Adenosine triphosphate and glutamate appear to be cotransmitters in primary afferent sensory neurons. Adenosine triphosphate, calcitonin gene-related peptide and substance P coexist in some sensory-motor nerves. A subpopulation of intramural enteric nerves provides non-adrenergic, non-cholinergic inhibitory innervation of gut smooth muscle. Three cotransmitters are involved, namely ATP, nitric oxide and vasoactive intestinal polypeptide. In recent years, studies have shown that ATP is released with ACh, noradrenaline, glutamate, gamma-aminobutyric acid, 5-hyroxytryptamine and dopamine in different subpopulations of neurons in the central nervous system.
Molecular Cloning: A Laboratory Manual (1989)

Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Mol
Scaling rules and parameter tuning procedure for analog design reuse in technology migration (2004)

In this paper a methodology for analog design reuse during technology scaling is proposed. First of all, analytical resizing rules are derived for MOS transistors working in saturation and triode regions. These rules, however, do not account for parasitic effects that, especially in submicron technologies, lead to inaccurate scaling. For this reason, for example, DC gain and unity gain frequency may substantially differ from the original. In order to compensate these inaccuracies, a tuning procedure based on SPICE simulations is proposed. Finally, the migration and tuning procedures are validated and simulation results are compared scaling down a Miller OTA from 0.25 /spl mu/m to 0.15 /spl mu/m technology.
Role of the β1 Subunit in the Function and Stability of the 20S Proteasome in the Hyperthermophilic ArchaeonPyrococcus furiosus (2006)

ABSTRACT The hyperthermophilic archaeon Pyrococcus furiosus genome encodes three proteasome component proteins: one α protein (PF1571) and two β proteins (β1-PF1404 and β2-PF0159), as well as an ATPase (PF0115), referred to as proteasome-activating nucleotidase. Transcriptional analysis of the P. furiosus dynamic heat shock response (shift from 90 to 105°C) showed that the β1 gene was up-regulated over twofold within 5 minutes, suggesting a specific role during thermal stress. Consistent with transcriptional data, two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that incorporation of the β1 protein relative to β2 into the 20S proteasome (core particle [CP]) increased with increasing temperature for both native and recombinant versions. For the recombinant enzyme, the β2/β1 ratio varied linearly with temperature from 3.8, when assembled at 80°C, to 0.9 at 105°C. The recombinant α+β1+β2 CP assembled at 105°C was more thermostable than either the α+β1+β2 version assembled at 90°C or the α+β2 version assembled at either 90°C or 105°C, based on melting temperature and the biocatalytic inactivation rate at 115°C. The recombinant CP assembled at 105°C was also found to have different catalytic rates and specificity for peptide hydrolysis, compared to the 90°C assembly (measured at 95°C). Combination of the α and β1 proteins neither yielded a large proteasome complex nor demonstrated any significant activity. These results indicate that the β1 subunit in the P. furiosus 20S proteasome plays a thermostabilizing role and influences biocatalytic properties, suggesting that β subunit composition is a factor in archaeal proteasome function during thermal stress, when polypeptide turnover is essential to cell survival.
Smart-Cut: A New Silicon On Insulator Material Technology Based on Hydrogen Implantation and Wafer Bonding*1 (1997)

An alternative route to existing silicon on insulator (SOI) material technologies such as SIMOX (separation by implanted oxygen) and BESOI (bonded and etch-back SOI) is the new Smart-Cut process, which appears to be a good candidate to achieve ULSI criteria. The Smart-Cut process involves two technologies: wafer bonding and ion implantation associated with a temperature treatment which induces a in-depth splitting of the implanted wafer. The details of the Smart-Cut process, the physical phenomena involved in the different technological steps such as hydrogen implantation related mechanisms and wafer bonding are discussed. The characteristics of the final structure in terms of thickness homogeneity, crystalline defects, surface microroughness, and electrical characterization are presented. Other applications of this process are also highlighted.
Preferential Inhibition of Akt and Killing of Akt-Dependent Cancer Cells by Rationally Designed Phosphatidylinositol Ether Lipid Analogues (2004)

Activation of the PI3k/Akt pathway controls key cellular processes and contributes to tumorigenesis in vivo, but investigation of the PI3k/Akt pathway has been limited by the lack of specific inhibitors directed against Akt. To develop Akt inhibitors, we used molecular modeling of the pleckstrin homology (PH) domain of Akt to guide synthesis of structurally modified phosphatidylinositol ether lipid analogues (PIAs). Here, we characterize the biochemical and cellular effects of PIAs. Of 24 compounds tested, five PIAs with modifications at two sites on the inositol ring inhibited Akt with IC(50)s < 5 micro M. Molecular modeling identified putative interactions of PIAs with the phosphoinositide-binding site in the PH domain of Akt, and growth factor-induced translocation of Akt to the plasma membrane was inhibited by PIA administration. Inhibition of Akt occurred rapidly and was maintained for hours. PIAs decreased phosphorylation of many downstream targets of Akt without affecting upstream kinases, such as PI3k or phosphoinositide-dependent kinase-1, or members of other kinase pathways such as extracellular signal-regulated kinase. Importantly, PIAs increased apoptosis 20-30-fold in cancer cell lines with high levels of endogenous Akt activity but only 4-5-fold in cancer cell lines with low levels of Akt activity. These studies identify PIAs as effective Akt inhibitors, and provide proof of principle for targeting the PH domain of Akt.
Effects of Baicalein Isolated from Scutellaria baicalensis Radix on Adhesion Molecule Expression Induced by Thrombin and Thrombin Receptor Agonist Peptide in Cultured Human Umbilical Vein Endothelial Cells (2001)

We examined the effects of various flavonoids isolated from the roots of Scutellaria baicalensis Georgi on adhesion molecule expression induced by thrombin and thrombin receptor agonist peptide (SFLLRNPNDKYEPF, TRAP) in cultured human umbilical vein endothelial cells. Thrombin and thrombin receptor agonist peptide induced endothelial leukocyte adhesion molecule-1 (ELAM-1) expression. Intercellular adhesion molecule-1 (ICAM-1) expression was also induced by thrombin, but not by TRAP. Baicalein isolated from Scutellariae Radix inhibited ELAM-1 expression induced by thrombin and thrombin receptor agonist peptide dose-dependently, with 50% inhibitory concentrations (IC50) of 5.53 +/- 1.68 microM and 2.44 +/- 1.08 microM, respectively. Furthermore, baicalein inhibited thrombin-induced ICAM-1 expression with an IC50 of 9.67 +/- 1.28 microM. In addition, baicalein inhibited the expressions of ELAM-1 and ICAM-1 stimulated by protein kinase C (PKC) activator phorbol myristate acetate (PMA).
Lipid Peroxides and Superoxide Dismutase (SOD) Induction in Skin Inflammatory Diseases, and Treatment with SOD Preparations (1989)

In the skin ulcer or severely inflamed and erosive lesions induced due to burn, wounds and other dermatitides, lipid peroxides were markedly increased, with resultant cytotoxic effects in situ. Generally, superoxide dismutase (SOD), which scavenges oxygen radicals or inhibits lipid peroxidation, is adapted to be induced (increased) under oxygen toxicity. For the treatment of not only systemic inflammatory diseases but also skin ulcer lesions, especially due to burn and wounds, liposomal-encapsulated SOD injection was effective. Topical application of free Mn-SOD or Cu, Zn-SOD extracted from bovine, bacterial and other species except for human was also dramatically effective in skin lesions; a burnt patient who was advised to undergo skin transplantation showed complete healing with free SOD cream. In addition, topical application of low molecular weight antioxidants, AOA or Bio-harmony, also showed remarkable effectiveness in these skin lesions. However, SOD dissolved in the vehicle containing greater amounts of vaselinum album (white petrolatum) rapidly lost its activity, and that dissolved in the vehicle with large quantities of water lost its activity within 3 months. In conclusion, SOD should be dissolved in the vehicle before use, however, low molecular weight antioxidant cream can be commercially sold because it does not lose its activity for long periods.
Modeling Web applications using Java and XML related technologies (2003)

The purpose of this paper is to propose visual models for a Web application using Java and XML related technologies. We consider a Web application that uses 3-tier architecture and each tier is distributed onto Web, application, and data base servers. In the Web applications, various Java and XML related technologies are employed in the form of different languages: client-side script language, server-side script language, object-oriented programming language, markup language, style sheet language, data language, schema definition language, etc. When diverse technologies are applied to a Web application, software engineers need to understand how the components using the technologies are interrelated in terms of software architecture. By applying the RUP with UML to the Web application, we show how visual models can be constructed for the Web application. Even, we show that we can model components in the Web application using non object-oriented languages such as script, markup, style sheet, schema definition, and data languages. In order to compare the modeling complexity of components, we propose a degree of modeling complexity based upon two criteria - the degree of language dependency and the degree of location dependency. Through the comparison criteria, we try to quantify how relatively difficult it is for us to model which components. Based upon the degree of modeling complexity, it is relatively difficult to model XHTML among components using non object-oriented languages. Among Java related technologies, since JSP has a higher degree of language dependency and a higher degree of location dependency, it is more difficult for us to model the JSP components.