gebhart/patrepeval huggingface dataset:
A role for CCN3 (NOV) in calcium signalling (2002)

The interaction of CCN3 with S100A4 may account, in part, for the association of CCN3 with carcinogenesis and its pattern of expression in normal conditions. The increased intracellular calcium concentrations induced by CCN3 and CCN2 both involve different processes, among which voltage independent calcium channels might be of considerable importance in regulating the calcium flux associated with cell growth control, motility, and spreading. These observations assign for the first time a biological function to the CCN3 protein and point out a broader role for the CCN proteins in calcium ion signalling.
Biosynthesis and Posttranslational Regulation of Human IL-12 (2000)

IL-12 is a heterodimeric proinflammatory cytokine consisting of a light alpha-chain, formerly defined as p35, disulfide-linked to a heavier beta-chain, formerly defined as p40. The beta-chain is also produced in large excess in a free form, and disulfide-linked beta-chain homodimers with anti-inflammatory effects are produced in the mouse. We analyzed the biosynthesis and glycosylation of IL-12 in human monocytes, and in a cell line stably transfected with IL-12 alpha and beta genes (P5-0.1). The IL-12 heterodimer and free beta-chain were immunoprecipitated from supernatants and cell lysates of metabolically labeled cells and resolved in SDS-PAGE. Whereas the beta-chain showed similar pI pattern whether in the free form or associated in the heterodimer, either in the secreted or intracellular form, the alpha-chain in the secreted heterodimer was much more acidic than that present in the intracellular heterodimer. Deglycosylation experiments with neuraminidase and Endo-F combined with two-dimensional PAGE of single bands of the intracellular vs extracellular IL-12 heterodimer revealed that the alpha-chain was extensively modified with sialic acid adducts to N-linked oligosaccharides before secretion. N-glycosylation inhibition by tunicamycin (TM) did not alter free beta-chain secretion, while preventing the IL-12 heterodimer assembling and secretion. Pulse-chase experiments indicated that IL-12 persists intracellularly for a long period as an immature heterodimer, and that glycosylation is the regulatory step that determines its secretion. beta-chain disulfide-linked homodimers were observed in TM-treated P5-0.1 cells, but in neither TM-treated nor untreated monocytes.
Detection, analysis and matching of hair (2005)

We develop computational models for measuring hair appearance for comparing different people. The models and methods developed have applications to person recognition and face image indexing. An automatic hair detection algorithm is described and results reported. A multidimensional representation of hair appearance is presented and computational algorithms are described. Results on a dataset of 524 subjects are reported. Identification of people using hair attributes is compared to eigenface-based recognition along with a joint, eigenface-hair based identification.
Alphavirus-Based Vaccines (2014)

Alphavirus vectors have demonstrated high levels of transient heterologous gene expression both in vitro and in vivo and, therefore, possess attractive features for vaccine development. The most commonly used delivery vectors are based on three single-stranded encapsulated alphaviruses, namely Semliki Forest virus, Sindbis virus and Venezuelan equine encephalitis virus. Alphavirus vectors have been applied as replication-deficient recombinant viral particles and, more recently, as replication-proficient particles. Moreover, in vitro transcribed RNA, as well as layered DNA vectors have been applied for immunization. A large number of highly immunogenic viral structural proteins expressed from alphavirus vectors have elicited strong neutralizing antibody responses in multispecies animal models. Furthermore, immunization studies have demonstrated robust protection against challenges with lethal doses of virus in rodents and primates. Similarly, vaccination with alphavirus vectors expressing tumor antigens resulted in prophylactic protection against challenges with tumor-inducing cancerous cells. As certain alphaviruses, such as Chikungunya virus, have been associated with epidemics in animals and humans, attention has also been paid to the development of vaccines against alphaviruses themselves. Recent progress in alphavirus vector development and vaccine technology has allowed conducting clinical trials in humans.
Influence of underlayer reflection on optical proximity effects in sub-quarter-micron lithography (1998)

The relationship between the optical proximity effect (OPE) and the underlayer reflection has been investigated by using negative and positive resists in sub-quarter-micrometer lithography. A new evaluation method that uses the center exposure dose of the ED-window (1-2) is been proposed. This technique takes the manufacturing margin into consideration and can be used to obtain the common ED-window of isolated and density patterns. The negative resists show a small critical dimension variation between isolated and density lines (CD bias) with the most suitable exposure dose and best focus conditions. However, the common ED-window of the isolated and density patterns is poor in terms of the manufacturing margin. Furthermore, the (sigma) dependence of the negative resist is too weak to improve the manufacturing margin. The effect of the underlayer reflection on the CD bias of the negative resist is significant in our experiment. On the other hand, the positive resist shows strong (sigma) dependence. Because the influence of the underlayer reflection on the positive resist is small, it is important to optimize (sigma) when improving the CD bias for the positive resist. In order to compare the negative and positive resists under equivalent conditions, a resist development simulation was used. The simulation results show the negative resist could be capable of high performance.
Serum midkine levels are increased in patients with various types of carcinomas (2000)

The level of expression of midkine (MK), a heparin-binding growth factor, is increased in many types of human carcinomas. An enzyme-linked immunoassay, which utilizes a combination of rabbit and chicken antibodies revealed that serum MK level in the controls (n = 135) was 0.154 +/- 0.076 (mean +/- SD) ng ml(-1)with an apparent cut-off value as 0.5 ng ml(-1). Serum MK level was significantly elevated in the cancer patients (n = 150) (P< 0.001); 87% of the patients showed levels of more than 0.5 ng ml(-1). All ten types of cancer examined showed a similar profile of serum MK level. There was no or weak correlation between C-reactive protein level, a marker of inflammation, and serum MK level. Furthermore, in case of gastric carcinoma and lung carcinoma, patients with stage I carcinoma already showed elevated serum MK levels. The present results indicated that serum MK could serve as a general tumour marker with a good potential for clinical application.
Infrared extinction properties of gold nanoshells (1999)

Gold nanoshells, nanoparticles consisting of a silica core coated with a thin gold shell, exhibit a strong optical resonance that depends sensitively on their core radius and shell thickness. Gold nanoshells have been fabricated with a peak optical extinction that can be varied across the near-infrared region of the spectrum (800 nm–2.2 μm). Multipolar plasmon resonances are clearly resolvable in the extinction spectra and agree well with electromagnetic theory. Additional resonances due to particle aggregation are also observed. The frequency agile infrared properties of these nanoparticles make them particularly attractive for a range of technologically important applications.
Functional screening of traditional antidepressants with primary cortical neuronal networks grown on multielectrode neurochips (2006)

We optimized the novel technique of multielectrode neurochip recordings for the rapid and efficient screening of neuroactivity. Changes in the spontaneous activity of cultured networks of primary cortical neurons were quantified to evaluate the action of drugs on the firing dynamics of complex network activity. The multiparametric assessment of electrical activity changes caused by psychoactive herbal extracts from Hypericum, Passiflora and Valeriana, and various combinations thereof revealed a receptor-specific and concentration-dependent inhibition of the firing patterns. The spike and burst rates showed significant substance-dependent effects and significant differences in potency. The effects of specific receptor blockades on the inhibitory responses provided evidence that the herbal extracts act on gamma-amino butyric acid (GABA) and serotonin (5-HT) receptors, which are recognized targets of pharmacological antidepressant treatment. A biphasic effect, serotonergic stimulation of activity at low concentrations that is overridden by GABAergic inhibition at higher concentrations, is apparent with Hypericum alone and the triple combination of the extracts. The more potent neuroactivity of the triple combination compared to Hypericum alone and the additive effect of Passiflora and Valeriana suggest a synergy between constituent herbal extracts. The extracts and their combinations affected the set of derived activity parameters in a concomitant manner suggesting that all three constituent extracts and their combinations have largely similar modes of action. This study also demonstrates the sensitivity, selectivity and robustness of neurochip recordings for high content screening of complex mixtures of neuroactive substances and for providing multiparametric information on neuronal activity changes to assess the therapeutic potential of psychoactive substances.
Research on the interaction between RMB interest rate, exchange rate fluctuations and Chinese capital market (2009)

Biotinylated complementary DNA (cDNA) and RNA probes were prepared from a specific and highly conserved section of the foot-and-mouth disease virus (FMDV) genome coding for the RNA-dependent RNA polymerase. Hybridization was conducted on FMDV-infected, bovine enterovirus (BEV)-infected, and noninfected swine kidney cell cultures. The detection system utilized the enzyme system streptavidin-alkaline phosphatase, the substrate phosphate, and the chromogen nitroblue tetrazolium. Intense cytoplasmic granular staining was present at 2 and 4 hr postinfection (hpi), with less staining observed at 24 hpi. The staining was specific for FMDV, as indicated by a lack of staining of noninfected cells and BEV-infected cells. With the RNA probe, positive cells were detected up to the highest viral dilution assayed, which was approximately 96 TCID50. The cDNA probe was slightly less sensitive, detecting positive cells at 10-fold lower dilutions. This technique could prove useful in the diagnosis of foot-and-mouth disease in animals or in the detection of FMDV in biologics submitted for importation.
CENE (2014)

Ce titre de Cène voudrait rassembler plusieurs significations, dont la religieuse, selon nous, accompagne les autres sans les contredire. Il célèbre, bien sûr, la table conviviale que Jean-Paul Resweber nous a appris à ne pas laisser dégrader en fast food. Le titre indique aussi son exigence que la table soit table ouverte : non pas scène de la communauté représentée, mais cène de participation à laquelle chacun est convié à sa façon et selon ses talents. Et le titre enfin convoque à la table...
COATING SYSTEMS FOR NON-FERROUS METAL SUBSTRATES--ALUMINUM (1998)

This article presents a discussion of paint systems over aluminum substrates and the many applications in which that metal finds utility, focusing on the use and coating of aluminum within the general sphere of protective coatings. In the transportation industry, aluminum is used for lightweight automotive components, truck and trailer bodies, recreation vehicles, high-speed trains, and aircraft (air frame and skin as well as other structural parts). Aluminum has been used widely on rockets, missiles, and space vehicles. In ship building, the metal is used in the construction of a wide range of vessels from tiny, aluminum-hulled pleasure craft, to the superstructure of oceangoing liners. The range also includes numerous fishing vessels and warships. Maximum corrosion resistance is secured from the purest aluminum. For many engineering applications, because of its low strength, aluminum must be alloyed. All of these alloys have reduced corrosion resistance compared with the pure aluminum, although the magnesium alloys are better in this respect than are the others and are preferred for hulls on pleasure craft. High-strength alloys based on copper or zinc are less corrosion-resistant, but are favored in aircraft applications because of their strength. Aluminum surfaces to be coated must be cleaned and pretreated before priming. The most effective types of pretreatment employ chromate solutions. Historically, aluminum has been successfully coated after anodizing, conversion coating, or wash priming with a variety of coatings, most typically alkyds, phenolics, vinyls, and acrylics. Topcoats for aircraft are almost always aliphatic polyurethanes.
Efficient Identification of Phosphorylation by Mass Spectrometric Phosphopeptide Fingerprinting (2008)

We describe a rapid and efficient method for the identification of phosphopeptides, which we term mass spectrometric (MS) phosphopeptide fingerprinting. The method involves quantitative comparison of proteolytic peptides from native versus completely dephosphorylated proteins. Dephosphorylation of serine, threonine, and tyrosine residues is achieved by in-gel treatment of the separated proteins with hydrogen fluoride (HF). This chemical dephosphorylation results in enrichment of those unmodified peptides that correspond to previously phosphorylated peptides. Quantitative comparison of the signal-to-noise ratios of peaks in the treated versus untreated samples are used to identify phosphopeptides, which can be confirmed and further studied by tandem mass spectrometry (MS/MS). We have applied this method to identify eight known phosphorylation sites of Xenopus Aurora A kinase, as well as several novel sites in the Xenopus chromosome passenger complex (CPC).
An Application of the Stille Coupling for the Preparation of Arylated Phthalonitriles and Phthalocyanines. (1999)

The substituted phthalonitriles 4-phenylphthalonitrile (2a), 4-(2,5-dimethoxyphenyl) phthalonitrile(2b) and 2-(3,4-dicyanophenyl)-4-methylpyridine(2c) have been prepared in good yields from 4-iodop ...
Azimuthal Turing Patterns, Bright and Dark Cavity Solitons in Kerr Combs Generated With Whispering-Gallery-Mode Resonators (2013)

We investigate the formation of cavity solitons in crystalline whispering-gallery-mode disk resonators that are pumped in different dispersion regimes. In the Fourier domain, these dissipative structures correspond to specific types of mode-locked Kerr optical frequency combs. Depending on the sign of the second-order chromatic dispersion and on the pumping conditions, we show that either bright or dark cavity solitons can emerge, and we show that these two regimes are associated with characteristic spectral signatures that can be discriminated experimentally. We use the Lugiato-Lefever spatiotemporal formalism to investigate the temporal dynamics leading to the formation of these azimuthal solitons, as well as the emergence of Turing patterns. The theoretical results are in excellent agreement with experimental measurements that are obtained using calcium and magnesium fluoride disk resonators pumped near 1550 nm.
Acquired and Inherited Lipodystrophies (2004)

Lipodystrophies, heterogeneous acquired or inherited disorders characterized by the selective loss of adipose tissue, predispose affected patients to insulin resistance and its attendant complications. This review focuses on the clinical features, underlying pathogenetic mechanisms, and management of various types of acquired and inherited lipodystrophies.
HIGH RESOLUTION BACKSCATTER ELECTRON (BSE) IMAGING OF IMMUNOGOLD WITH IN-LENS AND BELOW-THE-LENS FIELD EMISSION SCANNING ELECTRON MICROSCOPES (1999)

High resolution backscatter electron (BSE) imaging of colloidal gold with field emission scanning electron microscopy (FESEM) depends upon selection of accelerating voltage, beam current, working distance between specimen and backscatter detector, type of backscatter detector, plus the nature and thickness of the metal coating used to improve conductivity and signal collection. BSE imaging of a gold standard (6 nm, 12 nm, and 18 nm colloidal gold) was investigated with in-lens FESEM using a yttrium-aluminum garnet (YAG) scintillator detector and with below-the-lens FESEM using solid state and scintillator detectors, with the former providing the best imaging. Thin metal coatings (<1 nm) of platinum did not interfere with detection of the gold standard with in-lens FESEM so this thickness of platinum was used to test the BSE imaging of 12 nm gold with either solid state or scintillator detectors in below-the-lens FESEM. Due to the thin metal coatings used, a “charging” phenomenon was seen with secondary electron imaging, but this was negligible with BSE imaging because of their higher energy level. Highest BSE resolution was attained on the gold standard and biological samples with in-lens FESEM, but with below-lens FESEM, the labeling of surface molecules with 12 nm immunogold was successfully detected on coated cells at working distances (WD) of 6-20 mm, although shorter WD provided better quality images.
Overcoming Parallax and Sampling Density Issues in Image Mosaicing of Non-Planar Scenes (2007)

Image mosaicing constructs a wide field-of-view result from multiple source frames. In order to ensure a perceptually correct result, mosaicing typically requires either a planar or near-planar scene, parallax-free camera motion between source frames, or a dense sampling of the scene. When these conditions are not satisfied, various artifacts may result. A novel mosaicing approach is introduced that overcomes these limitations, building on the techniques of image-based rendering and manifold mosaicing, while permitting the synthesis of an effective mosaic of a non-planar scene from a sparse set of translated cameras. Our method first generates a series of intermediate virtual frames to reduce the disparities between neighboring images. Next, a series of vertical slices are chosen from the array of both real and virtual frames and connected according to a cost function that maximizes the similarity between adjacent slices. Experimental results indicate significant improvements over competing methods.
The Curious Case of Neural Text Degeneration (2019)

Despite considerable advancements with deep neural language models, the enigma of neural text degeneration persists when these models are tested as text generators. The counter-intuitive empirical observation is that even though the use of likelihood as training objective leads to high quality models for a broad range of language understanding tasks, using likelihood as a decoding objective leads to text that is bland and strangely repetitive. In this paper, we reveal surprising distributional differences between human text and machine text. In addition, we find that decoding strategies alone can dramatically effect the quality of machine text, even when generated from exactly the same neural language model. Our findings motivate Nucleus Sampling, a simple but effective method to draw the best out of neural generation. By sampling text from the dynamic nucleus of the probability distribution, which allows for diversity while effectively truncating the less reliable tail of the distribution, the resulting text better demonstrates the quality of human text, yielding enhanced diversity without sacrificing fluency and coherence.
Mitochondrial tRNA import – the challenge to understand has just begun (2009)

Mitochondrial translation is important for the synthesis of proteins involved in oxidative phosphorylation, which yields the bulk of the ATP made in cells. During evolution most mitochondria-containing organisms have lost tRNA genes from their mitochondrial genomes. Thus, to support the essential process of nuanced mitochondrial translation, mechanisms to actively transport tRNAs from the cytoplasm across the mitochondrial membranes into the mitochondrion have evolved. Here, we review the currently known tRNA import mechanisms, comment on recent discoveries of various import factors, and suggest a rationale for forces that lie behind the evolution of mitochondrial tRNA import.
Molecular Cloning of a Novel T Cell-directed CC Chemokine Expressed in Thymus by Signal Sequence Trap Using Epstein-Barr Virus Vector (1996)

Precursors of most secreted and cell surface molecules carry signal sequences at their amino termini. Here we describe an efficient signal sequence trap method and isolation of a novel CC chemokine. An expression library was constructed by inserting 5' portion-enriched cDNAs from phytohemagglutinin-stimulated peripheral blood mononuclear cells into upstream of signal sequence-deleted CD4 cDNA in an Epstein-Barr virus shuttle vector. After electroporation into Raji cells, CD4 antigen-positive cells were enriched by repeated cell sorting and plasmids were recovered in Escherichia coli. Out of 100 plasmid clones examined, 42 clones directed expression of CD4 antigen on the cell surface. Among them were signal sequences of CD6, beta2-microglobulin, MGC-24, and T cell receptor epsilon-chain, and at least four novel potential signal sequences. A cDNA clone encoding a novel CC chemokine was isolated by using one of the trapped fragments. The gene designated as TARC from Thymus and Activation-Regulated Chemokine was expressed transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells and constitutively in thymus. Radiolabeled recombinant TARC specifically bound to T cell lines and peripheral T cells but not to monocytes or granulocytes. The binding of radiolabeled TARC to the high-affinity receptor (Kd, 2.1 nM) on Jurkat was displaced by TARC but not by interleukin-8, MIP-1alpha, RANTES, or MCP-1. TARC also bound to the promiscuous chemokine receptor on erythrocytes (Kd, 17 nM). TARC induced chemotaxis in T cell lines Hut78 and Hut102. Pretreatment of Hut78 with pertussis toxin abolished the TARC-induced cell migration. Collectively, T cells express a highly selective receptor for TARC that is coupled to pertussis toxin-sensitive G-protein. TARC may a factor playing important roles in T cell development in thymus as well as in trafficking and activation of mature T cells.
Genomic Profiles in Stage I Primary Non Small Cell Lung Cancer Using Comparative Genomic Hybridization Analysis of cDNA Microarrays (2004)

To investigate the genomic aberrations that are involved in lung tumorigenesis and therefore may be developed as biomarkers for lung cancer diagnosis, we characterized the genomic copy number changes associated with individual genes in 14 tumors from patients with primary non small cell lung cancer (NSCLC). Six squamous cell carcinomas (SQCAs) and eight adenocarcinomas (ADCAs) were examined by high-resolution comparative genomic hybridization (CGH) analysis of cDNA microarray. The SQCAs and ADCAs shared common frequency distributions of recurrent genomic gains of 63 genes and losses of 72 genes. Cluster analysis using 57 genes defined the genomic differences between these two major histologic types of NSCLC. Genomic aberrations from a set of 18 genes showed distinct difference of primary ADCAs from their paired normal lung tissues. The genomic copy number of four genes was validated by fluorescence in situ hybridization of 32 primary NSCLC tumors, including those used for cDNA microarray CGH analysis; a strong correlation with cDNA microarray CGH data emerged. The identified genomic aberrations may be involved in the initiation and progression of lung tumorigenesis and, most importantly, may be developed as new biomarkers for the early detection and classification of lung cancer.