gebhart/patrepeval huggingface dataset:
Reproducción asistida en equinos: aportes desde la teoría (2010)

Assisted reproductive techniques (ART) have developed rapidly in recent years in equine production. The purpose of this review is to mention and discuss the main TRA currently used in horses. In Colombia, are routinely used techniques such as artificial insemination (AI) (using fresh, chilled or cryopreserved semen) and embryo transfer (ET), with the interest of increasing the offspring of certain animals or improving reproductive performance of them. The oocytes transfer (OT) and gamete intrafallopian transfer (GIFT) are used in some countries in mares with reproductive dysfunction; these techniques are less applicable in our country. The production of in vitro embryos, together with Intracytoplasmic Sperm Injection (ICSI) or cloning by nuclear transfer have been made in equines in basic research, or commercially in some countries with high costs that now, do not represent a tool for the clinical area in the country, but are of great interest for understanding the physiology, reproductive disorders and development in the equine specie.
Patterning π-Conjugated Polymers (2001)

π-Conjugated polymers show promise as active materials in application areas such as microelectronics, electro-optics, opto-electronics, and photonics. A critical feature in this emerging technology is device fabrication and the reproducible deposition of active material. This review focuses on current trends in the spatial deposition of conjugated polymers.
Role of IL-1alpha and IL-1beta in ischemic brain damage. (2001)

The cytokine interleukin-1 (IL-1) has been strongly implicated in the pathogenesis of ischemic brain damage. Evidence to date suggests that the major form of IL-1 contributing to ischemic injury is IL-1beta rather than IL-1alpha, but this has not been tested directly. The objective of the present study was to compare the effects of transient cerebral ischemia [30 min middle cerebral artery occlusion (MCAO)] on neuronal injury in wild-type (WT) mice and in IL-1alpha, IL-1beta, or both IL-1alpha and IL-1beta knock-out (KO) mice. Mice lacking both forms of IL-1 exhibited dramatically reduced ischemic infarct volumes compared with wild type (total volume, 70%; cortex, 87% reduction). Ischemic damage compared with WT mice was not significantly altered in mice lacking either IL-1alpha or IL-1beta alone. IL-1beta mRNA, but not IL-1alpha or the IL-1 type 1 receptor, was strongly induced by MCAO in WT and IL-1alpha KO mice. Administration (intracerebroventricularly) of recombinant IL-1 receptor antagonist significantly reduced infarct volume in WT (-32%) and IL-1alpha KO (-48%) mice, but had no effect on injury in IL-1beta or IL-1alpha/beta KO mice. These data confirm that IL-1 plays a major role in ischemic brain injury. They also show that chronic deletion of IL-1alpha or IL-1beta fails to influence brain damage, probably because of compensatory changes in the IL-1 system in IL-1alpha KO mice and changes in IL-1-independent mediators of neuronal death in IL-1beta KO mice.
Optimizing the Bituminous Concrete Mixes for Fatigue Performance (2008)

The objective of the present research work is to study the influence of aggregate gradation on fatigue performance and of Bituminous Concrete (BC) mixes, and to optimize the bituminous concrete mix for fatigue performance of within the specification band which offers longer fatigue life. Five aggregate gradations for an bituminous concrete mix falling within the specification band limit for bituminous surface course are considered. Repeated load indirect tensile fatigue test is carried out at 30, 35 and 40 °C using gyratory compacted cylindrical specimens. Laboratory fatigue test results are used for developing the optimization model considering stiffness modulus, horizontal tensile strain and air void as the constraints. Sensitivity analysis is also carried out to study the effect of variation in constraints values on fatigue life.
On the mobile radio capacity increase through SDMA (2002)

In a cellular mobile radio system an SDMA component can be implemented for the reuse of radio channels physically incorporated by time, frequency or code slots. In this paper we will present simulation results on an SDMA downlink channel allocation scheme operating in a typical urban mobile radio cell. Based on the resulting blocking probabilities we will give estimations on the capacity increase which can be expected after adding an SDMA component to a conventional mobile radio system.
Quantitative confocal spectroscopy &#x2014; rectifying the limitations of single molecule detection (2008)

Quantitative confocal spectroscopy (QCS) is a new implementation of single molecule detection that allows highly quantitative and accurate analysis of single fluorescent molecules. It rectifies the limitations of standard confocal spectroscopy by significantly enhancing mass detection efficiency and observation volume uniformity while preserving high signal to noise ratio and single molecule sensitivity. This is accomplished by incorporation of a cylindrical lens and a novel microfabricated confocal aperture to perform a 1-D expansion of the observation volume such that it spans the entire cross-section of a typical microchannel. First, QCS is theoretically compared to standard SMD using a combination of optical modeling and Monte Carlo simulation. Then the performance of QCS is experimentally validated against standard SMD using PicoGreen stained pBR322 DNA.
Mutagenesis of Plants Overexpressing CONSTANS Demonstrates Novel Interactions among Arabidopsis Flowering-Time Genes (2000)

CONSTANS (CO) promotes flowering of Arabidopsis in response to long photoperiods. Transgenic plants carrying CO fused with the cauliflower mosaic virus 35S promoter (35S::CO) flowered earlier than did the wild type and were almost completely insensitive to length of day. Genes required for CO to promote flowering were identified by screening for mutations that suppress the effect of 35S::CO. Four mutations were identified that partially suppressed the early-flowering phenotype caused by 35S::CO. One of these mutations, suppressor of overexpression of CO 1 (soc1), defines a new locus, demonstrating that the mutagenesis approach is effective in identifying novel flowering-time mutations. The other three suppressor mutations are allelic with previously described mutations that cause late flowering. Two of them are alleles of ft, indicating that FT is required for CO to promote early flowering and most likely acts after CO in the hierarchy of flowering-time genes. The fourth suppressor mutation is an allele of fwa, and fwa soc1 35S::CO plants flowered at approximately the same time as co mutants, suggesting that a combination of fwa and soc1 abolishes the promotion of flowering by CO. Besides delaying flowering, fwa acted synergistically with 35S::CO to repress floral development after bolting. The latter phenotype was not shown by any of the progenitors and was most probably caused by a reduction in the function of LEAFY. These genetic interactions suggest models for how CO, FWA, FT, and SOC1 interact during the transition to flowering.
Selective adsorption of phosphoproteins on gel-immobilized ferric chelate (1986)

Ferric ions are very strongly adsorbed to iminodiacetic acid substituted agarose. This firmly immobilized complex acts as a selective immobilized metal affinity adsorbent for phosphoproteins. Chromatography based on this principle is illustrated by the adsorption-desorption behavior of egg yolk phosvitin before and after dephosphorylation as well as by the change in the chromatographic pattern before and after enzymic phosphorylation of selected histones. The strength of binding is dependent on the phosphate content. The difference in binding before and after phosphorylation of a single amino acid residue is demonstrated. Affinity elution can be accomplished by inclusion in the buffer of phosphoserine or a displacing metal ion such as Mg2+.
Software protection and simulation on oblivious RAMs (1996)

Software protection is one of the most important issues concerning computer practice. There exist many heuristics and ad-hoc methods for protection, but the problem as a whole has not received the theoretical treatment it deserves. In this paper, we provide theoretical treatment of software protection. We reduce the problem of software protection to the problem of efficient simulation on oblivious RAM. A machine is oblivious if thhe sequence in which it accesses memory locations is equivalent for any two inputs with the same running time. For example, an oblivious Turing Machine is one for which the movement of the heads on the tapes is identical for each computation. (Thus, the movement is independent of the actual input.) What is the slowdown in the running time of a machine, if it is required to be oblivious? In 1979, Pippenger and Fischer showed how a two-tape oblivious Turing Machine can simulate, on-line, a one-tape Turing Machine, with a logarithmic slowdown in the running time. We show an analogous result for the random-access machine (RAM) model of computation. In particular, we show how to do an on-line simulation of an arbitrary RAM by a probabilistic oblivious RAM with a polylogaithmic slowdown in the running time. On the other hand, we show that a logarithmic slowdown is a lower bound.
A Regulatory Mechanism for the Balanced Synthesis of Membrane Phospholipid Species inEscherichia coli (1996)

The mechanism that assures the balanced synthesis of zwitterionic (phosphatidylethanolamine) and acidic phospholipids (phosphatidylglycerol and cardiolipin) in Escherichia coli has been examined by genetically manipulating the two enzymes at the biosynthetic branch point, i.e., phosphatidylglycerophosphate synthase, encoded by pgsA, and phosphatidylserine synthase, encoded by pssA. A mutant in which the most part of the pssA gene was replaced with a drug resistance gene lacked phosphatidylserine synthase and phosphatidylethanolamine and required divalent metal ions for growth, as did a previously reported insertion-inactivated pssA mutant. When this mutant harbored a plasmid containing a Bacillus subtilis gene that encodes membrane-bound phosphatidylserine synthase, the phosphatidylethanolamine content was dependent on its activity, in contrast to that with the soluble E. coli counterpart. A defective mutation, pgsA3, caused reductions not only in acidic-phospholipid synthesis but also in phosphatidylethanolamine synthesis, despite the normal level of phosphatidylserine synthase activity. These results, together with previous observations, indicate that phosphatidylserine synthesis requires the membrane-associated form of phosphatidylserine synthase, which is related to the membrane-levels of acidic phospholipids, thus yielding balanced compositions of zwitterionic and acidic phospholipids.
Epidemiology and Clinical Impact of Parainfluenza Virus Infections in Otherwise Healthy Infants and Young Children < 5 Years Old (1997)

Over a 20-year period in a population of otherwise healthy children, respiratory viruses have been cultured from nasal wash specimens from each child with a clinically significant respiratory illness. Since efforts are underway to develop vaccines for prevention of illness due to parainfluenza virus (PIV) type 3, the epidemiologic characteristics of PIVs were reviewed, and the population size necessary to demonstrate vaccine efficacy was estimated. A population of 1429 children was followed through early childhood. PIVs were isolated from 286 samples, 17.4% of positive viral cultures. PIV-3 was the most common: 10% of the children had at least one symptomatic, culture-proven PIV-3 infection. PIV-3 was endemic during the study period, while the other two PIVs, PIV-1 and -2, caused biennial flu epidemics. Only four PIV-related hospitalizations were seen. The efficacy of a PIV-3 vaccine could be demonstrated in a trial of 600 carefully monitored children vaccinated by 3 months and followed to 15 months of age.
Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes (2004)

We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphilis-causing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phage-related or of unknown function.
Applications of phytic acid (1983)

Abstract Phytic acid (myo‐inositol hexaphosphate) constitutes 1‐3% of most plant seeds. Its tremendous chelating potential and its effects on the absorption of polycationic nutrilites such as Ca 2+ , Zn 2+ and Fe 3+ have been the subject of intense investigation for several decades. Yet in the American literature there is virtually no information available on other chemical properties of phytic acid or on its beneficial utilization. This review summarizes the present medical, dental, nutritional and industrial applications of phytic acid and suggests additional novel uses for this inexpensive and easily obtained chemical.
Neu oncogene expression in ovarian tumors: a quantitative study. (1992)

We studied neu mRNA expression by slot blot analysis and protein product expression by capture ELISA and immunohistochemistry in 57 primary and metastatic ovarian neoplasms, two paraovarian leiomyosarcomas, and eight normal ovaries. Some 61% of ovarian tumors but none of the paraovarian neoplasms or normal ovaries overexpressed neu mRNA. A total of 96% of the ovarian tumors that overexpressed neu were of epithelial type. Epithelial ovarian tumors had significantly higher amounts of the neu oncogene product as determined by capture ELISA than either germ cell and stromal tumors or normal ovaries (p less than 0.025). Different subtypes of ovarian carcinomas had significantly different amounts of neu oncogene product as measured by capture ELISA; endometrioid tumors had the highest, and poorly differentiated carcinomas not otherwise specified had the lowest (p less than 0.025). ELISA values, mRNA overexpression, and immunohistochemical staining intensity did not correlate with stage at diagnosis or architectural or nuclear grade in ovarian tumors. We conclude that capture ELISA is a simple, effective way to measure the neu oncogene protein product and that there is a good correlation between ELISA levels and immunohistochemical staining intensity. However, ELISA values did not correlate with stage or histologic prognostic factors in ovarian neoplasms.
Evaluation of the perforating veins of the lower extremity using high resolution duplex imaging. (1991)

The purpose of this study was to evaluate duplex imaging as a means of assessing perforating veins in patients with longstanding venous stasis ulceration. Thirty patients with nonhealing venous stasis ulcers and twenty normal volunteers serving as controls were evaluated with a standard 8-MHz imaging probe and integrated pulsed Doppler. The internal diameter of perforating veins was measured and perforators were grouped into one of four categories based on functional criteria: competent perforator with venous flow (C); incompetent perforator with venous flow (IC); competent perforator with arteriovenous flow (C-AVC); incompetent perforator with arteriovenous flow (IC-AVC). Sixteen of the patients with ulcers were subsequently studied by ascending venography and in 11 patients, intraoperative identification of perforating veins was made during the Rob procedure. In this subset of patients the vessel was categorized as competent or incompetent regardless of flow signal. There were significant differences in mean perforator diameter between ulcer patients and normal volunteers. Duplex imaging identified 93 perforators and venography a total of 70. In all 16 extremities, duplex imaging made the diagnosis of significant perforating vein incompetence, verified by venography. Intraoperative findings demonstrated an excellent correlation with duplex imaging. We believe duplex imaging to be a promising new modality for evaluation of the perforating veins of the lower extremity.
A Novel Mechanism for the Formation of Direct Strand Breaks upon Anaerobic Photolysis of Duplex DNA Containing 5-Bromodeoxyuridine (1996)

The mechanism of UV-induced cleavage of duplex DNA containing 5-bromodeoxyuridine under anaerobic conditions was investigated via product analysis and kinetic isotope effect measurements using gel electrophoresis. KIEs strongly suggest that strand breaks result from abstraction of the C2‘ hydrogen atom from the 5‘-adjacent nucleotide by deoxyuridin-5-yl (1). It is proposed that under anaerobic conditions the C2‘ radical is subsequently oxidized by the cation radical of adenine. The resulting carbocation undergoes 1,2-hydride migrations from C1‘ and C3‘ competitively, giving rise to cleavage products containing 3‘-phosphate and the novel, labile 3‘-ketone (4). The identity of the latter product is supported by electrospray mass spectrometric analysis of the crude photolysate. The results also suggest that the formation of alkaline labile lesions proceed via hydrogen atom abstraction from the C2‘ position by 1.
Natural Fibers, Biopolymers, and Biocomposites (2005)

Natural Fibers, Biopolymers, and Biocomposites: An Introduction A.K. Mohanty, M. Misra, L.T. Drzal, S.E. Selke, B.R. Harte, and G.Hinrichsen Plant Fibers as Reinforcement for Green Composites A. Bismarck, S. Mishra, and T. Lampke Processing of Bast Fiber Plants for Industrial Application F. Munder, C. Furll, and H.Hempel Recent Developments in Retting and Measurement of Fiber Quality in Natural Fibers: Pro and Cons R.B. Dodd and D.E. Akin Alternative Low-Cost Biomass for the Biocomposites Industry D.D. Stokke Fiber-Matrix Adhesion in Natural Fiber Composites P.J. Herrera Franco and A. Valadez-Gonzalez Natural Fiber Composites in Automotive Applications B.C. Suddell and W.J. Evans Natural Fiber Composites for Building Applications B. Singh and M. Gupta Thermoset Biocomposites D. Ray and J. Rout Thermoplastic Wood Fiber Composites S. Godavarti Bamboo-Based Ecocomposites and Their Potential Applications K. Kitagawa, U. S. Ishiaku, M. Mizoguchi, and H. Hamada Oil Palm Fiber-Thermoplastic Composites H.D. Rozman, Z.A. Mohd Ishak, and U.S. Ishiaku Natural Fiber-Rubber Composites and Their Applications S. Joseph, M. Jacob, and S. Thomas Straw-Based Biomass and Biocomposites X. Mo, D. Wang, and X.S. Sun Sorona(R)Polymer: Present Status and Future Perspectives J.V. Kurian Polylactic Acid Technology D.E. Henton, P. Gruber, J. Lunt, and J. Randall Polylactide-Based Biocomposites D. Plackett and A. Sodergard Bacterial Polyester-Based Biocomposites: A Review A. Hodzic Cellulose Fiber-Reinforced Cellulose Esters: Biocomposites for the Future G. Toriz, P. Gatenholm, B.D. Seiler, and D. Tindall Starch Polymers: Chemistry, Engineering, and Novel Products B.-S. Chiou, G.M. Glenn, S.H. Imam, M.K. Inglesby, D.F. Wood, and W.J. Orts Lignin-Based Polymer Blends and Biocomposite Materials S. Kubo, R.D. Gilbert, and J.F. Kadla Soy Protein-Based Plastics, Blends, and Composites A.K. Mohanty, W. Liu, P. Tummala, L.T. Drzal, M. Misra, and R.Narayan Synthesis, Properties, and Potential Applications of Novel Thermosetting Biopolymers from Soybean and Other Natural Oils F. Li and R.C. Larock Houses Using Soy Oil and Natural Fibers Biocomposites M.A. Dweib, A. O'Donnell, R.P. Wool, B. Hu, and H.W. Shenton III Biobased Polyurethanes and Their Composites: Present Status and Future Perspective J.-P. Latere Dwan'Isa, A.K. Mohanty, M. Misra, and L.T. Drzal Cellulose-Based Nanocomposites L. Berglund How Sustainable Are Biopolymers and Biobased Products? The Hope, the Doubts, and the Reality M. Patel and R. Narayan Index
Laser lipolysis versus traditional liposuction for fat removal (2009)

Liposuction is second only to breast augmentation as the most commonly performed cosmetic procedure in the USA. Liposuction removes fat through very small skin incisions, with atraumatic, blunt-tipped cannulas. Simplicity, quality of results and relatively rare complications are but a few benefits that explain its success. Its main limitation is the minimal skin retraction achieved after the procedure. Laser lipolysis is based on a thermal effect. The laser can vaporize, melt tissues and coagulate blood vessels. More importantly, the laser stimulates the formation of collagen in the region, enhancing skin elasticity and promoting skin contraction in the treated areas. This review aims to describe traditional liposuction and laser lipolysis, and discusses the difference between the two techniques.
Nitric Oxide in the Central Nervous System (1995)

The majority of the data on nitric oxide (NO) in the central nervous system (CNS) relies on histochemical and immunohistochemical evidence concerning the distribution of the nitric oxide synthase (NOS), its inhibition by specific antagonists and its co-localization with the receptor enzyme guanylate cyclase (GC) in the same functional region. All three isoforms, endothelial (eNOS), neural (nNOS) and macrophage type inducible (iNOS), are of importance to the normal and pathological function of the CNS. In nNOS gene deleted mice eNOS seems to contribute to the maintenance of neuronal function. NO may contribute to synaptic plasticity as a retrograde mediator that is released by postsynaptic NMDA-receptor activation. Microglia contains membrane-bound inducible iNOS that may be important in host defence function. Glia and pericytes surrounding the blood vessels contain GC that is stimulated by NO released from endothelium and nerve endings. Excessive production of highly reactive NO may be responsible for the neurotoxicity mediated by NMDA receptors that contributes to the symptomatology of strokes and neurodegenerative diseases. Moreover, after initial stimulation by cytokines, large amounts of NO produced by iNOS in the microglia (brain-based macrophages) may cause cellular damage.