gebhart/patrepeval huggingface dataset:
Formation of polypeptide-dye multilayers by electrostatic self-assembly technique (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of polypeptide-dye multilayers by electrostatic self-assembly techniqueThomas M. Cooper, Angela L. Campbell, and Robert L. CraneCite this: Langmuir 1995, 11, 7, 2713–2718Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/la00007a061https://doi.org/10.1021/la00007a061research-articleACS PublicationsRequest reuse permissionsArticle Views758Altmetric-Citations174LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The chemistry of cyclic vinyl ethers. 6. Total synthesis of polyether ionophore antibiotics of the calcimycin (A-23187) class (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe chemistry of cyclic vinyl ethers. 6. Total synthesis of polyether ionophore antibiotics of the calcimycin (A-23187) classRobert K. Boeckman Jr., Andre B. Charette, Theodros Asberom, and Brian H. JohnstonCite this: J. Am. Chem. Soc. 1991, 113, 14, 5337–5353Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/ja00014a029https://doi.org/10.1021/ja00014a029research-articleACS PublicationsRequest reuse permissionsArticle Views1477Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
CEA/CD3 bispecific antibody MEDI-565/AMG 211 activation of T cells and subsequent killing of human tumors is independent of mutations commonly found in colorectal adenocarcinomas (2014)

Individual or combinations of somatic mutations found in genes from colorectal cancers can redirect the effects of chemotherapy and targeted agents on cancer cell survival and, consequently, on clinical outcome. Novel therapeutics with mechanisms of action that are independent of mutational status would therefore fulfill a current unmet clinical need. Here the CEA and CD3 bispecific single-chain antibody MEDI-565 (also known as MT111 and AMG 211) was evaluated for its ability to activate T cells both in vitro and in vivo and to kill human tumor cell lines harboring various somatic mutations commonly found in colorectal cancers. MEDI-565 specifically bound to normal and malignant tissues in a CEA-specific manner, and only killed CEA positive cells. The BiTE® antibody construct mediated T cell-directed killing of CEA positive tumor cells within 6 hours, at low effector-to-target ratios which were independent of high concentrations of soluble CEA. The potency of in vitro lysis was dependent on CEA antigen density but independent of the mutational status in cancer cell lines. Importantly, individual or combinations of mutated KRAS and BRAF oncogenes, activating PI3KCA mutations, loss of PTEN expression, and loss-of-function mutations in TP53 did not reduce the activity in vitro. MEDI-565 also prevented growth of human xenograft tumors which harbored various mutations. These findings suggest that MEDI-565 represents a potential treatment option for patients with CEA positive tumors of diverse origin, including those with individual or combinations of somatic mutations that may be less responsive to chemotherapy and other targeted agents.
Optical and electronic properties of fluorene‐based copolymers and their sensory applications (2012)

Abstract A series of novel, fluorene‐based conjugated copolymers, poly[(9,9‐bis{propenyl}‐9H‐fluorene) ‐co‐ (9,9‐dihexyl‐9H‐fluorene)] ( P1 ), poly[(9,9‐bis{carboxymethylsulfonyl‐propyl}fluorenyl‐2,7‐diyl) ‐co‐ (9,9‐dihexyl‐9H‐fluorene)] ( P2 ) and poly[(9,9‐dihexylfluorene) ‐co‐ alt‐(9,9‐bis‐(6‐azidohexyl)fluorene)] ( P3 ), are synthesized by Suzuki coupling reactions and their electrochemical properties, in the form of films, are investigated using cyclic voltammetry. The results reveal that the polymer films exhibit electrochromic properties with a pseudo‐reversible redox behavior; transparent in the neutral state and dark violet in the oxidized state. Among the three polymers, P2 possesses the shortest response time and the highest coloration efficiency value. These polymers emit blue light with a band gap value of around 2.9 eV and have high fluorescent quantum yields. Their metal ion sensory abilities are also investigated by titrating them with a number of different transition metal ions; all of these polymers exhibit a higher selectivity toward Fe 3+ ions than the other ions tested with Stern–Volmer constants of 4.41 × 10 6 M −1 , 3.28 × 10 7 M −1 , 1.25 × 10 6 M −1 , and 6.56 × 10 6 M −1 for P1 , P2 , water soluble version of P2 ( P2S ) and P3 , respectively. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
Deubiquitinating Enzyme Usp12 Is a Novel Co-activator of the Androgen Receptor (2013)

The androgen receptor (AR), a member of the nuclear receptor family, is a transcription factor involved in prostate cell growth, homeostasis, and transformation. AR is a key protein in growth and development of both normal and malignant prostate, making it a common therapeutic target in prostate cancer. AR is regulated by an interplay of multiple post-translational modifications including ubiquitination. We and others have shown that the AR is ubiquitinated by a number of E3 ubiquitin ligases, including MDM2, CHIP, and NEDD4, which can result in its proteosomal degradation or enhanced transcriptional activity. As ubiquitination of AR causes a change in AR activity or stability and impacts both survival and growth of prostate cancer cells, deubiquitination of these sites has an equally important role. Hence, deubiquitinating enzymes could offer novel therapeutic targets. We performed an siRNA screen to identify deubiquitinating enzymes that regulate AR; in that screen ubiquitin-specific protease 12 (Usp12) was identified as a novel positive regulator of AR. Usp12 is a poorly characterized protein with few known functions and requires the interaction with two cofactors, Uaf-1 and WDR20, for its enzymatic activity. In this report we demonstrate that Usp12, in complex with Uaf-1 and WDR20, deubiquitinates the AR to enhance receptor stability and transcriptional activity. Our data show that Usp12 acts in a pro-proliferative manner by stabilizing AR and enhancing its cellular function.
Proangiogenic Contribution of Adiponectin toward Mammary Tumor Growth In vivo (2009)

These observations highlight a novel important contribution of APN in mammary tumor development and angiogenesis, indicating that APN has potent angio-mimetic properties in tumor vascularization. However, in tumors deprived of APN, this antiangiogenic stress results in an adaptive response that fuels tumor growth through mobilization of circulating endothelial progenitor cells and the development of mechanisms enabling massive cell proliferation despite a chronically hypoxic microenvironment.
Detecting mass-mailing worm infected hosts by mining DNS traffic data (2005)

The Domain Name System (DNS) is a critical infrastructure in the Internet; thus, monitoring its traffic, and protecting DNS from malicious activities are important for security in cyberspace. However, it is often difficult to determine whether a DNS query is caused by malicious or normal activity, because information available in DNS traffic is limited.We focus on the activities of mass-mailing worms and propose a method to detect hosts infected by mass-mailing worms by mining DNS traffic data. Our method begins with a small amount of a priori knowledge about a signature query. By assuming that queries sent by most hosts that have sent the signature query of worms have been sent by worm behavior, we detect infected hosts using Bayesian estimation.We apply our method to DNS traffic data captured at one of the largest commercial Internet Service Providers in Japan, and the experimental result indicates that an 89% reduction of mail exchange queries can be achieved with the method.
Characterization of a Non-Superconducting Cuprate, Bi2Sr2YCu2O8.5 (1988)

A new Bi-containing cuprate, Bi 2 Sr 2 YCu 2 O 8.5 , was found in the course of substitution study for high T c compounds Bi 2 Sr 2 CaCu 2 O 8+ y . Bi 2 Sr 2 YCu 2 O 8.5 is a semiconductor and undergoes an antiferromagnetic transition at about 13 K which is considered to originate from the ordering of Cu in the CuO 2 planes. An electron diffraction study revealed the existence of a superstructure. The unit cell taking account of the superstructure is 1×8×1 of the original unit cell (5.47×5.43×30.18 Å 3 ). Analogy of three kinds of oxide systems, (La 1- x Sr x ) 2 CuO 4 , YBa 2 Cu 3 O 6+ x , and Bi 2 Sr 2 Ca x Y 1- x Cu 2 O 8+ y , is discussed.
Oligonucleotide—Poly-L-ornithine Conjugates: Binding to Complementary DNA and RNA (1993)

On the basis of the reported enhanced antisense activity of polylysine-oligonucleotide conjugates, a synthetic 12-mer oligodeoxyribonucleotide has been coupled at its 5' terminus to a series of positively charged (delta-ornithine)n cysteine peptides. Binding between the nucleic acid-peptide conjugate and its complementary DNA target sequence was detected by the impact of complexation on the melting temperature (Tm). It was found that the Tm for the nucleic acid-peptide gradually increased with increasing net charge on the conjugated peptide. Site-directed cleavage with RNase H demonstrates that the peptide-modified oligomer also hybridizes with its RNA target sequence. Increased affinity for target mRNA with net charge was shown by a cell-free translation arrest assay.
Bicarbonate and dichloroacetate: Evaluating pH altering therapies in a mouse model for metastatic breast cancer (2011)

DB combination therapy did not appear to enhance the effect of chronic oral bicarbonate. The anti-tumor effect of DCA may be dependent on the cancer model. Our studies suggest DCA efficacy is unpredictable as a cancer therapy and further studies are necessary to determine the role of this agent in the tumor microenvironment.
Original Papers. Success and Predictors of Blood Pressure Control in Diverse North American Settings: The Antihypertensive and Lipid‐Lowering Treatment to Prevent Heart Attack Trial (ALLHAT) (2002)

These data demonstrate that blood pressure may be controlled in two thirds of a multiethnic hypertensive population in diverse practice settings. Systolic blood pressure is more difficult to control than diastolic blood pressure, and at least two antihypertensive medications are required for most patients to achieve blood pressure control. It is likely that the majority of people with hypertension could achieve a blood pressure <140/90 mm Hg with the antihypertensive medications available today.
Interaction of synthetic analogs of distamycin and netropsin with nucleic acids. Does curvature of ligand play a role in distamycin-DNA interactions? (1988)

Distamycin and netropsin, a class of minor groove binding nonintercalating agents, are characterized by their B-DNA and A-T base-specific interactions. To understand the conformational and chemical basis of the above specificities, the DNA-binding characteristics of a novel synthetic analogue of distamycin have been studied. The analogue, mPD derivative, has the requisite charged end groups and a number of potential hydrogen-bonding loci equal to those of distamycin. The difference in the backbone curvatures of the ligands, distamycin, the mPD derivative, and NSC 101327 (another structurally analogous compound), is a major difference between these ligands. UV and CD spectroscopic studies reported here show the following salient features: The mPD derivative recognizes only B-DNA, to which it binds via the minor groove. On the other hand, unlike distamycin, it binds with comparable affinities to A-T and G-C base pairs in a natural DNA. These DNA-binding properties are compared with those reported earlier for distamycin and NSC 101327 [Zimmer, Ch., & Wahnert, U. (1986) Prog. Biophys. Mol. Biol. 47, 31-112]. The backbone structures of these three ligands were compared to show the progressive decrease in curvatures in the order distamycin, mPD derivative, and NSC 101327. The plausible significance of the backbone curvature vis-à-vis the characteristic B-DNA and AT-specific binding of distamycin is discussed. To our knowledge, this is the first attempt (with a model synthetic analogue) to probe the possible influence of backbone curvature upon the specificity of interactions of the distamycin class of groove-binding ligands with DNA.
Hypertrophic cardiomyopathy mutations in MYBPC3 dysregulate myosin (2019)

The mechanisms by which truncating mutations in <i>MYBPC3</i> (encoding cardiac myosin-binding protein C; cMyBPC) or myosin missense mutations cause hypercontractility and poor relaxation in hypertrophic cardiomyopathy (HCM) are incompletely understood. Using genetic and biochemical approaches, we explored how depletion of cMyBPC altered sarcomere function. We demonstrated that stepwise loss of cMyBPC resulted in reciprocal augmentation of myosin contractility. Direct attenuation of myosin function, via a damaging missense variant (F764L) that causes dilated cardiomyopathy (DCM), normalized the increased contractility from cMyBPC depletion. Depletion of cMyBPC also altered dynamic myosin conformations during relaxation, enhancing the myosin state that enables ATP hydrolysis and thin filament interactions while reducing the super relaxed conformation associated with energy conservation. MYK-461, a pharmacologic inhibitor of myosin ATPase, rescued relaxation deficits and restored normal contractility in mouse and human cardiomyocytes with <i>MYBPC3</i> mutations. These data define dosage-dependent effects of cMyBPC on myosin that occur across the cardiac cycle as the pathophysiologic mechanisms by which <i>MYBPC3</i> truncations cause HCM. Therapeutic strategies to attenuate cMyBPC activity may rescue depressed cardiac contractility in patients with DCM, whereas inhibiting myosin by MYK-461 should benefit the substantial proportion of patients with HCM with <i>MYBPC3</i> mutations.
Cellular proteins binding to the first Src homology 3 (SH3) domain of the proto-oncogene product c-Crk indicate Crk-specific signaling pathways. (1995)

The widely expressed c-Crk protein, composed of one SH2 and two SH3 domains, lacks an apparent catalytic domain, suggesting that it functions through the formation of specific complexes with other proteins. Bacterially expressed c-Crk formed in vitro highly stable complexes via the first SH3 domain [SH3(N)]. Most prominent were a 185 kDa protein of unknown identity (p185), Sos- immunoreactive bands of 170 kDa (p170) and 145 to 155 kDa bands, corresponding to the recently cloned C3G protein. p170 also bound to Ash/Grb2 and Nck while p185 and C3G bound only to Crk. Additional Crk binding proteins were found in hematopoietic cells, particularly the myeloid-monocytic lineage. The protein binding properties of Crk were subsequently compared to CRKL, the product of a homologous but distinct gene, and found to be very similar. The binding of two guanine nucleotide exchange factors, Sos and C3G, to Crk and CRKL indicates that Ras or related proteins likely play a role in signaling through Crk family proteins.
Structure−Activity Relationships of Acetylcholinesterase Noncovalent Inhibitors Based on a Polyamine Backbone. 3. Effect of Replacing the Inner Polymethylene Chain with Cyclic Moieties (2004)

In the present paper we expanded SAR studies of 3, the ethyl analogue of the AChE inhibitor caproctamine (2), by investigating the role of its octamethylene spacer separating the two amide functions through the replacement with dipiperidine and dianiline moieties. Compounds 4 and 8 were the most interesting of the two series of compounds. Compound 4 was the most potent AChE inhibitor with a pIC50 value of 8.48 +/- 0.02, while displaying also significant muscarinic M2 antagonistic activity (pKb value of 6.18 +/- 0.20). The availability of a suitable assay allowed us to verify whether 2, 3, 4, and 8 inhibit AChE-induced Abeta aggregation. Although all four derivatives caused a mixed type of AChE inhibition (active site and PAS), only 4 and 8, which bear an inner constrained spacer, were able to inhibit AChE-induced Abeta aggregation to a greater extent than donepezil. Clearly, the ability of an AChE inhibitor, based on a linear polyamine backbone, to bind both AChE sites may not be a sufficient condition to inhibit also AChE-induced Abeta aggregation. Dipiperidine derivative 4 emerged as a valuable pharmacological tool and a promising lead compound for new ligands to investigate and, hopefully, treat Alzheimer's disease.
Prevention Cocktails: Combining Tools to Stop HIV's Spread (2005)

With no vaccine in sight, AIDS researchers are testing a range of surprising biomedical interventions.
Gut Microbiota Signatures Predict Host and Microbiota Responses to Dietary Interventions in Obese Individuals (2014)

This proof-of-principle study introduces the first potential microbial biomarkers for dietary responsiveness in obese individuals with impaired metabolic health, and reveals the potential of microbiota signatures for personalized nutrition.
Use of Amnion for the Treatment of Stevens-Johnson Syndrome (1986)

Stevens-Johnson syndrome (toxic epidermal necrolysis) has similar pathophysiologic characteristics to extensive partial-thickness burns. Successful treatment of a 6-year-old with this syndrome denuding 95% of her body surface, with 6.5 square feet of amnion is reported.
Nanocrystalline LiF via microemulsion systems (1999)

LiF has been prepared in nanocrystalline form by means of microemulsion-mediated precipitation of NH 4 F and LiOAc. Grain growth during the intermicellar exchange, i.e. Ostwald ripening due to the comparatively high solubility of LiF, was suppressed by a strict control of the reaction time. For short times a flowmixer with a certain tube length was used. The reaction was stopped by coagulation in acetone. On heating, the by-product, NH 4 OAc, transforms into acetamide which can easily be extracted. The resulting nano-LiF was isolated as a powder; it exhibits grain sizes between 25 and 70 nm, depending on the reaction time.
Efficient Sonogashira Reactions of Aryl Bromides with Alkynylsilanes Catalyzed by a Palladium/Imidazolium Salt System (2002)

A palladium/imidazolium chloride system has been used to mediate the cross-coupling reaction of aryl halides with alkynylsilanes. The combination of 3 mol % Pd(OAc)2 and 6 mol % IMes·HCl in the presence of Cs2CO3 as base proves to be a highly efficient system in assisting coupling of aryl bromides with alkynylsilanes.
Quantitiative determination of glycosylated hemoglobin A1 by agar gel electrophoresis. (1980)

Journal Article Quantitiative determination of glycosylated hemoglobin A1 by agar gel electrophoresis. Get access L Menard, L Menard Search for other works by this author on: Oxford Academic Google Scholar M E Dempsey, M E Dempsey Search for other works by this author on: Oxford Academic Google Scholar L A Blankstein, L A Blankstein Search for other works by this author on: Oxford Academic Google Scholar H Aleyassine, H Aleyassine Search for other works by this author on: Oxford Academic Google Scholar M Wacks, M Wacks Search for other works by this author on: Oxford Academic Google Scholar J S Soeldner J S Soeldner Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 26, Issue 11, 1 October 1980, Pages 1598–1602, https://doi.org/10.1093/clinchem/26.11.1598 Published: 01 October 1980
Determination of Galectin-3 Messenger Ribonucleic Acid Overexpression in Papillary Thyroid Cancer by Quantitative Reverse Transcription-Polymerase Chain Reaction (2002)

Galectin-3, a lectin-family protein that appears to be involved in malignant transformation, has been reported to be an accurate immunohistochemical marker for thyroid cancer. However, immunohistochemistry is a subjective method that can be difficult to apply to cytologic specimens. Therefore, we sought to develop an objective and quantitative assay to measure galectin-3 mRNA in thyroid tissue to enhance potential clinical use of galectin-3 in the molecular analysis of thyroid nodules. In this study, total RNA from 37 snap-frozen thyroid tissue specimens was isolated from eight papillary and nine follicular thyroid cancers, six follicular adenomas, seven adenomatoid nodules, and seven normal thyroid lobes from patients undergoing thyroidectomy. Normalized levels of galectin-3 mRNA, expressed as picograms per nanogram GAPDH mRNA, were higher in papillary carcinomas (3327 pg/ng) and follicular adenomas (1314 pg/ng) than in thyroid normal tissue (426 pg/ng; P = 0.0012 and 0.032, respectively). Galectin-3 mRNA levels were also higher in papillary cancers than in adenomatoid nodules (P = 0.0012). However, galectin-3 mRNA levels were not statistically greater in follicular carcinomas than either normal tissue or follicular adenomas (P = 0.068 and 0.12, respectively). In summary, in comparison to galectin-3 immunohistochemistry, quantitative measurement of galectin-3 mRNA appears useful in the identification of papillary thyroid cancers (PTCs) but does not appear to be useful in distinguishing follicular carcinomas from follicular adenomas.