gebhart/patrepeval huggingface dataset:
Flowfill-Process as a New Concept for Inter-Metal-Dielectrics (1998)

Various process sequences are established for depositing inter-metal-dielectrics (IMD).The most common insulating stack between metal layers is SOG / etch-back. The sequence is as follows: Silane-oxide deposition - Spin-on-glass application -etch-back of SOG - another silane-oxide deposition. This process flow has several draw-backs. It is time consuming, takes a lot of equipment and has got high COO, due to the complexity of process steps. As an alternative we investigated Flowfill. Flowfill is a new self planarising process for depositing silane oxide layers. The chemistry is very simple. It is a reaction of Silane and hydrogen peroxide, leading to a liquid intermediate. The gas phase reaction produces vaporised glass which condenses as a liquid on substrates cooled to approximately zero degrees Celsius. This liquid state is the main reason for reaching good gap fill and excellent planarity. After polymerisation, the resulting silica film contains a lot of water. This problem is solved by a heat treatment. In the Flowfill sequence the insulating block of layers between the metal lines is done in one System and in one go. Thus saving a lot of physical process time. The stack consists of three layers: base - flow - cap. The base layer treatment results in good adhesion for the following flow and a moisture barrier for the devices. The cap avoids cracks in the flow layer during heat treatment. After this annealing the cap is no more necessary and can be removed. Base and cap are deposited as PE-CVD silane oxide. The result is a competitive IMD. We are presenting a new type of IMD: the Flowfill - Process sequence and its advantages. The motivation of changing to Flowfill is lower cost of ownership. The shorter physical process time is also an important advantage in production of logical devices. The via-resistance is stable over the wafer.
DNA Sequence Analyses Support the Role of Interrupted Gap Repair in the Origin of Internal Deletions of the Maize Transposon, MuDR (1996)

Previous research has demonstrated that the autonomous Cy transposon can activate the excision of Mu transposons. To determine the relationship between Cy and the more recently described autonomous Mu transposon, MuDR, a Cy transposon inserted at the mutable a1 allele, a1-m5216, was isolated and cloned. DNA sequence analyses established that this Cy insertion is identical to MuDR (Mu9, GenBank accession No.: m76978.gb-pl). Therefore, Cy will henceforth be termed MuDR:Cy. Defective derivatives of MuDR:Cy were isolated that had lost their capacity to activate their own excision or the excision of a Mu7 transposon. Most of these derivatives are nonautonomous transposons because they can excise, but only in the presence of unlinked MuDR:Cy transposons. Physical mapping and DNA sequence analyses have established that six of these defective derivatives carry internal deletions. It has been proposed previously that such deletions arise via interrupted gap repair. The DNA sequences of the break points associated with all four sequenced deletions are consistent with this model. The finding that three of the excision-defective derivatives carry deletions that disrupt the coding region of the mudrA (but not the mudrB) transcript supports the view that mudrA plays a role in the excision of Mu transposons.
Activation of OX40 Signal Transduction Pathways Leads to Tumor Necrosis Factor Receptor-associated Factor (TRAF) 2- and TRAF5-mediated NF-κB Activation (1998)

We investigated the intracellular signaling of OX40, a member of the tumor necrosis factor receptor family. Activation of NF-kappaB in OX40-transfected HSB-2 cells was detected by electrophoretic mobility shift assay within 30 min after the binding of the ligand gp34. In vitro binding experiments showed that tumor necrosis factor receptor-associated factor (TRAF) 1, TRAF2, TRAF3, and TRAF5 but not TRAF4 associated with glutathione S-transferase-OX40 fusion protein. The cotransfection experiments using human embryo kidney cell derived HEK 293T cells showed that TRAF2, TRAF3, and TRAF5 associated with OX40 in vivo. Studies with OX40 deletion mutants demonstrated that the cytoplasmic portion consisting of amino acid sequence 256-263 (GGSFRTPI) was required for the association with TRAFs and NF-kappaB activation. The introduction of the dominant negative mutants of TRAF2 and TRAF5 into HSB-2-OX40 cells suppressed NF-kappaB activation in a dose-dependent manner. In addition, the introduction of TRAF3 together with the dominant negative mutants of TRAF2 or TRAF5 further reduced NF-kappaB activation. These results indicate that the NF-kappaB activation resulting from OX40 stimulation is mediated by both TRAF2 and TRAF5, and is likely to be negatively modulated by TRAF3.
Matrix metalloproteinases at a glance (2004)

The matrix metalloproteinases (MMPs) are one of the major families of proteinases that play key roles in the responses of cells to their microenvironment. Most notably the MMPs have the combined capacity to degrade all the components of the extracellular matrix. Besides modulating tissue structure
On the Size Range of Active Nucleation Cavities on a Heating Surface (1962)

The importance of surfare condition on nucleate boiling has long been recognized. It has also been known that only cavities of a narrow size range can be active nucleation sites. In order to define the size range of active cavities as a function of wall temperature or heat flux, a model is proposed. The model pictures a bubble nucleus at a site enveloped by a warm liquid. The nucleus will begin to grow into a bubble only when the surrounding liquid is sufficiently superheated. The time required for the liquid to attain this superheat is called the waiting period. The transfer of heat from the superheated liquid into the bubble is considered to be a transient conduction process. A cavity is considered effective only if the waiting period is finite. This criterion gives the limiting sizes of effective cavities. The equations show that maximum and minimum sizes of effective cavities are functions of subcooling, pressure of the system, physical properties, and the thickness of the superheated liquid layer. Comparison of theoretical prediction with experimental data from several sources was made. The fluids considered were ether, pentane, and water, with water under various degrees of subcooling. The theory did predict the incipience of boiling and size range of cavities successfully.
Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer (2004)

In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. Comparisons of expression profiles in primary human prostate cancer, adjacent normal prostate tissue, and a selection of other (nonprostate) normal human tissues, led to the identification of a set of clones that were judged as the best candidate markers of normal and/or malignant prostate tissue. DNA sequencing of the selected clones revealed that they included 10 genes that had previously been established as prostate markers: NKX3.1, KLK2, KLK3 (PSA), FOLH1 (PSMA), STEAP2, PSGR, PRAC, RDH11, Prostein and FASN. Following analysis of the expression patterns of all selected and sequenced genes through interrogation of SAGE databases, a further three genes from our clone set, HOXB13, SPON2 and NCAM2, emerged as additional candidate markers of human prostate cancer. Quantitative RT-PCR demonstrated the specificity of expression of HOXB13 in prostate tissue and revealed its ubiquitous expression in a series of 37 primary prostate cancers and 20 normal prostates. These results demonstrate the utility of this expression-microarray approach in hunting for new markers of individual human cancer types.
C 900—An advanced mobile radio telephone system with optimum frequency utilization (1984)

The paper presents a survey of a new approach to advanced system solutions for mobile radio telephone systems. It is shown that, by the combination of both radio techniques and microprocessor data capabilities, improved means are available for controlling the specific multiple propagation and interference influences within the mobile radio environment. The aim of the system structure is to achieve the best possible frequency channel utilization, system availability, and cost benefits for deployment and users if traffic demand grows. The paper shows the different types of switching functions in public land mobile networks. Problems, as caused purely by radio conditions, are controlled within a defined radio exchange function, allowing the optimization of individual cell parameters. It is shown that distance measurement is the most reliable means of detecting cell boundaries. New approaches for system control channels lead to synchronous operation of base stations. The system operates with off-air call setup and queueing to improve channel utilization. Full national and international roaming capabilities are provided. During all calls, continuous digital link control is maintained to allow the radio exchange function in call condition. This new approach leads to different types of handoff to suit specific situations. Speech scrambling provides subscriber privacy.
Acylated Plasminogen‐Streptokinase Activator Complex: A New Approach to Thrombolytic Therapy (1990)

Acylated plasminogen-streptokinase activator complex (APSAC; antistreplase) is an inactive complex of human plasminogen and streptokinase. When it is injected, a controlled deacylation of the catalytic center occurs, activating the complex so that thrombolysis may begin. This process extends the half-life of streptokinase, allowing for 4-6 hours of fibrinolytic activity. Anistreplase has demonstrated equivalent efficacy to intracoronary streptokinase with regard to reperfusion rates in acute myocardial infarction. In addition, patients have shown a 56% reduction in mortality at 28 days with anistreplase compared to heparin. The adverse effect profile of anistreplase includes minor bleeding and hematoma formation at the site of venipuncture, hypotensive and bradycardic episodes, arrhythmias, facial flushing, fever, and rarely, allergic reactions. Serious bleeding reactions are uncommon, with the frequency of cerebrovascular accident reported at 0.4-0.6%. The special advantage of anistreplase is its administration as a 30-U intravenous bolus injected over 5 minutes, eliminating the need for long infusions and increasing the ease of administration. Based on its efficacy and ease of administration, anistreplase may become the drug of choice in the emergency treatment of acute myocardial infarction.
Acute Vasoconstrictor Response to Intravenous Furosemide in Patients with Chronic Congestive Heart Failure (1985)

Hemodynamic and neurohumoral responses to acute diuretic therapy were measured in 15 patients with severe chronic heart failure given intravenous furosemide, 1.3 +/- 0.6 (SD) mg/kg body weight. Left ventricular pump function deteriorated by 20 minutes, as indicated by a fall in stroke volume index (27 +/- 8 to 24 +/- 7 mL/min X m2 body surface area, p less than 0.01) and an increase in left ventricular filling pressure (28 +/- 7 to 33 +/- 9 mm Hg, p less than 0.01). Increases occurred in heart rate (87 +/- 13 to 91 +/- 16 beats/min, p less than 0.01), mean arterial pressure (90 +/- 15 to 96 +/- 15 mm Hg, p less than 0.01), systemic vascular resistance (1454 +/- 394 to 1676 +/- 415 dynes X s X cm-5, p less than 0.01), plasma renin activity (9.9 +/- 8.5 to 17.8 +/- 16 ng/mL X h, p less than 0.05), plasma norepinephrine level (667 +/- 390 to 839 +/- 368 pg/mL, p less than 0.01), and plasma arginine vasopressin level (6.2 +/- 1.3 to 8.3 +/- 2.0 pg/mL, p less than 0.01). During the next 3.5 hours the patients had diuresis (2085 +/- 1035 mL) and the expected fall in filling pressure (28 +/- 7 to 22 +/- 10 mm Hg, p less than 0.01). Neurohumoral indicators also returned toward the control levels. Intravenous furosemide, in patients with severe chronic heart failure, is associated with acute pump dysfunction temporally related to activation of the neurohumoral axis.
Determining the Size and Shape Dependence of Gold Nanoparticle Uptake into Mammalian Cells (2006)

We investigated the intracellular uptake of different sized and shaped colloidal gold nanoparticles. We showed that kinetics and saturation concentrations are highly dependent upon the physical dimensions of the nanoparticles (e.g., uptake half-life of 14, 50, and 74 nm nanoparticles is 2.10, 1.90, and 2.24 h, respectively). The findings from this study will have implications in the chemical design of nanostructures for biomedical applications (e.g., tuning intracellular delivery rates and amounts by nanoscale dimensions and engineering complex, multifunctional nanostructures for imaging and therapeutics).
Labeling and Enzyme Studies of the Central Carbon Metabolism in Metallosphaera sedula (2010)

Metallosphaera sedula (Sulfolobales, Crenarchaeota) uses the 3-hydroxypropionate/4-hydroxybutyrate cycle for autotrophic carbon fixation. In this pathway, acetyl-coenzyme A (CoA) and succinyl-CoA are the only intermediates that can be considered common to the central carbon metabolism. We addressed the question of which intermediate of the cycle most biosynthetic routes branch off. We labeled autotrophically growing cells by using 4-hydroxy[1-¹⁴C]butyrate and [1,4-¹³C₁]succinate, respectively, as precursors for biosynthesis. The labeling patterns of protein-derived amino acids verified the operation of the proposed carbon fixation cycle, in which 4-hydroxybutyrate is converted to two molecules of acetyl-CoA. The results also showed that major biosynthetic flux does not occur via acetyl-CoA, except for the formation of building blocks that are directly derived from acetyl-CoA. Notably, acetyl-CoA is not assimilated via reductive carboxylation to pyruvate. Rather, our data suggest that the majority of anabolic precursors are derived from succinyl-CoA, which is removed from the cycle via oxidation to malate and oxaloacetate. These C₄intermediates yield pyruvate and phosphoenolpyruvate (PEP). Enzyme activities that are required for forming intermediates from succinyl-CoA were detected, including enzymes catalyzing gluconeogenesis from PEP. This study completes the picture of the central carbon metabolism in autotrophic Sulfolobales by connecting the autotrophic carbon fixation cycle to the formation of central carbon precursor metabolites.
Phenotypic and Functional Analysis at the Clonal Level of Infiltrating T Lymphocytes in Papillary Carcinoma of the Thyroid: Prevalence of Cytolytic T Cells with Natural Killer-Like or Lymphokine-Activated Killer Activity* (1989)

We investigated the phenotype and function of thyroid tumor-, metastatic lymph node-, and peripheral blood-derived T lymphocytes of four patients with papillary thyroid cancer. Both phenotypic analysis of freshly isolated cells and clonal analysis, using a high efficiency cloning technique, were performed. For comparison, intrathyroid and peripheral blood T lymphocytes derived from patients with autoimmune thyroid diseases (Graves' disease and Hashimoto's thyroiditis) have been studied. In papillary cancer, the phenotype of thyroid and lymph node-derived T lymphocytes did not differ from that of peripheral blood lymphocytes of the same patients or lymphocytes from normal peripheral blood. At variance with respect to autoimmune thyroid disease, activation markers were poorly represented. The functional analysis of T cell clones showed similar proportions of interleukin-2-producing (helper-inducer) clones in thyroid, lymph node, and peripheral blood, slightly lower than in Hashimoto's thyroiditis and slightly higher than in Graves' disease. With regard to effector function, we found lower proportion of clones with cytolytic activity in a lectin-dependent assay compared to that in Hashimoto's thyroiditis. Interestingly, however, the proportions of cytolytic clones displaying cytolytic activity against the neoplastic cell line K562 (natural killer-like activity) or fresh unrelated tumor cells (lymphokine-activated killer activity) were relatively high in thyroid cancer infiltrates.
Randomized, Double-Blind, Placebo-Controlled, Multicenter Study of Siltuximab in High-Risk Smoldering Multiple Myeloma (2019)

Although this study did not meet the prespecified protocol hypothesis criteria, data suggest that siltuximab may delay the progression of high-risk SMM.
Tiger Shark—A scalable file system for multimedia (1998)

Tiger Shark is a scalable, parallel file system designed to support interactive multimedia applications, particularly large-scale ones such as interactive television (ITV). Tiger Shark runs under the IBM AIX® operating system, on machines ranging from RS/6000™ desktop workstations to the SP2® parallel supercomputer. In addition to supporting continuous-time data, Tiger Shark provides scalability, high availability, and on-line system management, all of which are crucial in large-scale video servers. These latter features also enable Tiger Shark to support nonmultimedia uses, such as scientific computing, data mining, digital library, and scalable network file servers. Tiger Shark has been employed in a number of customer ITV trials. On the basis of experience obtained from these trials, Tiger Shark has recently been released in several IBM video-server products. This paper describes the architecture and implementation of Tiger Shark, discusses the experience gained from trials, and compares Tiger Shark to other scalable video servers.
Oral Protein Drug Delivery (1996)

Click to increase image sizeClick to decrease image sizeKey Words: absorptionbarriersendocytosisenhancersliposomesmicroparticulates
Filovirus Replication and Transcription (2007)

The highly pathogenic filoviruses, Marburg and Ebola virus, belong to the nonsegmented negative-sense RNA viruses of the order Mononegavirales. The mode of replication and transcription is similar for these viruses. On one hand, the negative-sense RNA genome serves as a template for replication, to generate progeny genomes, and, on the other hand, for transcription, to produce mRNAs. Despite the similarities in the replication/transcription strategy, filoviruses have evolved structural and functional properties that are unique among the nonsegmented negative-sense RNA viruses. Moreover, there are also striking differences in the replication and transcription mechanisms of Marburg and Ebola virus. This includes nucleocapsid formation, the structure of the genomic replication promoter, the protein requirement for transcription and the use of mRNA editing. In this article, the current knowledge of the replication and transcription strategy of Marburg and Ebola virus is reviewed, with focus on the observed differences.
Suppression of BRAF(V599E) in human melanoma abrogates transformation. (2003)

Activating mutations in the BRAF serine/threonine kinase are found in >70% of human melanomas, of which >90% are BRAF(V599E). We sought to investigate the role of the BRAF(V599E) allele in malignant melanoma. We here report that suppression of BRAF(V599E) expression by RNA interference in cultured human melanoma cells inhibits the mitogen-activated protein kinase cascade, causes growth arrest, and promotes apoptosis. Furthermore, knockdown of BRAF(V599E) expression completely abrogates the transformed phenotype as assessed by colony formation in soft agar. Similar targeting of BRAF(V599E) or wild-type BRAF in human fibrosarcoma cells that lack the BRAF(V599E) mutation does not recapitulate these effects. Moreover, these results are specific for BRAF, as targeted interference of CRAF in melanoma cells does not significantly alter their biological properties. Thus, when present, BRAF(V599E) appears to be essential for melanoma cell viability and transformation and, therefore, represents an attractive therapeutic target in the majority of melanomas that harbor the mutation.
Protein i: interference at protein level by intrabodies (2006)

Immunoglobulin molecules have long been used in biomedical research as in vitro tools for identification, purification and functional manipulation of target proteins. The specificity and diversity of immunoglobulins can be exploited to target a wide range of intracellular proteins by expressing them in vivo. Such antibody molecules, which are expressed intracellularly and directed to defined sub-cellular compartments, are termed 'intrabodies'. They represent a new and versatile tool that has the potential to manipulate diverse biological processes. Gene knockout, antisense and small interfering RNA knockdown techniques, are employed to characterize the function of many gene products and to validate these gene products as potential drug targets for novel therapeutics, but they have their limitations. The experimental results to date suggest that intrabodies represent a powerful alternative to modulate protein function and analyze its effects. Moreover, they can also be utilized to target specific domains of a particular target protein. Even though their clinical application might take a long time, they can play an important role for target identification and validation in functional genomics and/or proteomics. Here we review the latest advances in the concept, construction and applications of Intrabodies.
Approaches to the development of selective inhibitors of vascular smooth muscle cell proliferation (1993)

Abnormal proliferation of VSMC is a feature of atheromatous plaques and is responsible for obstructive neointimal lesions at the sites of mechanical or immunological intimal damage. We have discussed approaches to the development of specific inhibitors of VSMC proliferation based firstly on studies of the mechanism of action of known inhibitors and secondly on the identification of genes which are unique to and necessary for VSMC proliferation. The effects of angiotensin converting enzyme inhibitors, heparin and hexamethylene bisacetamide on VSMC proliferation have been discussed. Each of these types of agent has been shown to inhibit VSMC proliferation in vitro and/or in vivo. Examination of the mechanisms by which these agents inhibit VSMC proliferation reveals that transforming growth factor beta is an important mediator of their action and that the processes of de-differentiation and proliferation are independently regulated. Studies aimed at identifying genes involved in VSMC proliferation are at an early stage but have already provided strong evidence to support the hypothesis that VSMC in the vessel wall are heterogeneous and contain a subpopulation of cells with an enhanced proliferative capacity. Identification of the genes expressed by these cells may allow specific inhibitors of VSMC proliferation to be developed and may shed light on the pathogenesis of neointimal lesions.
New Iridium Complexes as Highly Efficient Orange–Red Emitters in Organic Light‐Emitting Diodes (2003)

Two new iridium complexes with pyrazine derivatives as ligands (see Figure) were synthesized. The electroluminescent devices based on these metal complexes emit orange–red light at ∼ 610 nm with exceedingly high brightness and external quantum, current, and power efficiencies.
Eukaryotic DNA Replication: Anatomy of an Origin (1993)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More