gebhart/patrepeval huggingface dataset:
Comparison of S100b protein with calmodulin: interactions with melittin and microtubule-associated .tau. proteins and inhibition of phosphorylation of .tau. proteins by protein kinase C (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComparison of S100b protein with calmodulin: interactions with melittin and microtubule-associated .tau. proteins and inhibition of phosphorylation of .tau. proteins by protein kinase CJacques Baudier, Daria Mochly-Rosen, Alexandra Newton, Sun Hee Lee, Daniel E. Koshland, Jr., and R. David ColeCite this: Biochemistry 1987, 26, 10, 2886–2893Publication Date (Print):May 19, 1987Publication History Published online1 May 2002Published inissue 19 May 1987https://pubs.acs.org/doi/10.1021/bi00384a033https://doi.org/10.1021/bi00384a033research-articleACS PublicationsRequest reuse permissionsArticle Views182Altmetric-Citations92LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
PAX8 and peroxisome proliferator-activated receptor gamma 1 gene expression status in benign and malignant thyroid tissues (2004)

These observations confirm that the PAX8-PPARgamma1 translocation characterizes a subset of thyroid follicular carcinomas but is not a specific marker of carcinoma and that its frequency is lower than that initially reported. Finally, immunohistochemistry is not a reliable method for the specific detection of the translocation, that can be specifically evidenced by Q RT-PCR.
High performance strained-Si p-MOSFETs on SiGe-on-insulator substrates fabricated by SIMOX technology (2003)

We have proposed a new MOSFET structure, strained-Si/Si/sub 0.9/Ge/sub 0.1/-on-Insulator (SSGOI) MOSFETs applicable to the sub-100 nm generation. This SSGOI structure was successfully fabricated by the combination of SIMOX technology and the Si re-growth technique. The strained-Si in SSGOI was found to have good crystal quality and very flat interfaces. SSGOI p-MOSFETs exhibited good FET characteristics. It was demonstrated, for the first time, that the hole mobility of the SSGOI p-MOSFETs is higher that of the universal mobility of conventional Si p-MOSFETs.
Penicillin combinations against multi-resistant urinary pathogens as an alternative to gentamycin treatment. (1976)

A total of 42 multi-resistant urinary pathogens, sensitive to gentamycin only, were investigated with regard to the effects of dicloxacillin and ampicillin or cloxacillin/flucloxacillin and amoxycillin in combination. A synergistic antibiotic effect with resulting low MIC levels was demonstrated for 81% of the strains. It was also found that a further increase in sensitivity might be achieved by adjustment of the pH level.
Generation of a human anti-idiotypic antibody that mimics the GD2 antigen. (1993)

In a phase 1 trial, patients with metastatic melanoma received the anti-GD2 murine mAb 14G2a. All patients developed human anti-14G2a antibodies including anti-Id antibodies. Peripheral blood MNCs from one such patient were fused with the murine myeloma cell line Ag8. Four human anti-14G2a secreting hybridomas were generated and the mAb product of one of the hybridomas was characterized. The human mAb 4B5 (hu-IgG, lambda) binds to the variable region of murine 14G2a (anti-Id). The 4B5 binds to the antigen-combining site of 14G2a and inhibits its binding to GD2 expressing Mel-21 cells. Rabbits were immunized with the human anti-Id 4B5. Sera from the immunized rabbits demonstrated anti-4B5 antibodies and anti-Mel-21 and anti-GD2 reactivity. Furthermore, rabbit sera competitively inhibited binding of 14G2a to Mel-21 cells. Rabbits immunized with 4B5 developed a DTH response when challenged with 4B5 antibody and Mel-21 cells. These studies demonstrate that the human anti-Id 4B5 mimics the GD2 antigen and is capable of eliciting both a humoral and cellular anti-GD2 immune response. This antibody could be potentially used as a human anti-Id vaccine in patients with malignant melanoma.
Comparison between kinematic and ground reaction force techniques for determining gait events during treadmill walking at different walking speeds (2010)

This study involved a comparison of kinematics-based and ground reaction force (GRF)-based event detection methods. The objectives were (1) to assess the difference between the determination of gait events from GRF-based and kinematic/ultrasound-based techniques and (2) to determine the effects of gait speed on the agreement between the two techniques. At combined speeds, the average of the true difference was 2.6+/-2.8 ms for heel strike and -1.3+/-2.4 ms for toe-off identification. A positive value indicated that GRF-based identification occurred before kinematics-based identification. The average of the true difference was -3.9+/-3.5 ms for the duration of stance; thus, the duration of stance as determined by the kinematics-based technique was shorter than that determined by GFR-based detection. Strong correlations (range 0.948-0.974) were found at all (slow, moderate, and fast) gait speeds. Near unity of slope of the linear regression line (range 0.955-1.008) was identified for the duration of stance between the two methods. Our results suggest that the agreement between the two event detection methods depended on gait speed, but the differences were small. The data determined using kinematic/ultrasound-based and GRF-based methods were comparable in healthy participants.
Rheumatoid Factor Specificity of a VH3-Encoded Antibody Is Dependent on the Heavy Chain CDR3 Region and Is Independent of Protein A Binding (1998)

Rheumatoid factors (RF) recognize conformational determinants located within the Fc portion of IgG. By analyzing a panel of monoclonal rheumatoid arthritis (RA)-derived RFs, we previously demonstrated that the somatically generated light chain complementarity-determining region 3 (CDR3) contributes to RF specificity. We have now generated a panel of heavy chain mutants of the B'20 Ab, a high affinity RA-derived IgM RF. B'20 also binds avidly to protein A and weakly to ssDNA and tetanus toxoid. B9601, a RF negative Ab that is highly homologous to B'20 but does not bind any of the Ags tested, and RC1, a low affinity polyreactive RF, were used to generate heavy chain mutants with framework (FR) and CDR switches. The mutated heavy chains were cotransfected into a myeloma cell line with the germline counterpart of the B'20 light chain, and the expressed Ig tested for antigenic specificity. We show that both RF specificity and polyreactivity of B'20 is dependent on its unique heavy chain CDR3 region. Replacement with a B9601 CDR3 shortened to the same length as the B'20 CDR3, and with only 5 amino acid differences, did not restore Fc binding. Conversely, absence of protein A binding of B9601 is due to the presence of a serine residue at position 82a in the B9601 heavy chain FR3 region. Together, our data suggest that Ig gene recombination events can generate B cells with autoantibody specificities in the preimmune repertoire. Abnormal release, activation, expansion, or mutation of such cells might all contribute to the generation of a high titer RF response in patients with RA.
Alanine and Glutamine Release from the Human Forearm: Effects of Glucose Administration (1985)

The effect of glucose infusion alone (175 mg/kg bolus dose followed by 4 mg min-1 kg-1 for 70 min) and in combination with forearm exercise on the exchange of glucose, alanine, glutamine and other metabolites and amino acids across forearm muscle was studied in six healthy individuals after an overnight fast. Arterial and deep venous blood was sampled and a mercury strain gauge plethysmograph was used to measure forearm blood flow. Total body energy expenditure and net glucose and fat oxidation were assessed by indirect calorimetry. The infusion of glucose increased the mean arterial blood glucose concentration from 4.95 +/- 0.19 (SEM) to a plateau of 9.6-9.9 mmol/l (P less than 0.01). The arterial blood concentrations of alanine and glutamine were not significantly altered but that of lactate increased from 0.50 +/- 0.02 to 0.65 +/- 0.05 mmol/l (P less than 0.02) and that of pyruvate increased from 46 +/- 5 to 72 +/- 6 mumol/l (P less than 0.01). In the resting state glucose administration did not significantly affect the lactate/pyruvate ratio in arterial or venous blood. Arterial plasma insulin concentration increased four-fold and total ketone body concentration decreased two- to three-fold. After glucose administration, alanine release was suppressed (in all subjects) from a mean value of 153 +/- 22 to 57 +/- 16 nmol min-1 100 ml-1 of forearm (P less than 0.02) whereas that of glutamine was not significantly affected (160 +/- 30 to 143 +/- 29 nmol min-1 100 ml-1 of forearm). Lactate release, like that of alanine, decreased, whereas pyruvate was slowly released in the basal state and was taken up during glucose administration (P less than 0.01). These changes were associated with a decrease in the uptake of total ketone bodies to one-fifth to one-tenth of that in the basal state. The net amino acid balance across the forearm muscle bed was negative throughout the study but decreased from a mean value of -567 in the basal state to -300 nmol min-1 100 ml-1 of forearm after glucose administration for 60 min. This was predominantly due to decreased release of effluxing amino acids, particularly alanine.(ABSTRACT TRUNCATED AT 400 WORDS)
Electrical signature of the deformation and dehydration of microgels during translocation through nanopores (2011)

The resistive-pulse sensing technique was used to investigate the deformation and dehydration of individual 570 nm radius poly(N-isopropylacrylamide-co-acrylic acid) microgels during their translocation through a glass membrane containing a single conical nanopore with orfice radii ranging from 200 to 700 nm. Microgel translocation rates were found to be dependent on both the applied pressure and the pore radius, and a translocation threshold pressure was found to be a function of the pore size. Importantly, current–time traces resulting from translocation events demonstrate changes in the conductivity of the microgel, due to compression and partial dehydration during translocation. A minimum nanopore-to-microgel radius ratio of ∼0.4 is observed for translocation, suggesting a theoretical limit imposed by the compressibility of the microgel and Columbic repulsion with the pore walls. Our results provide fundamental insight into microgels including their compressibility and conductivity, as well as the ability of soft particles to permeate porous membranes and pass through voids of dimensions smaller than the particles themselves.
thy/liv‐SCID‐hu Mice: A System for Investigating the In Vivo Effects of Multidrug Therapy on Plasma Viremia and Human Immunodeficiency Virus Replication in Lymphoid Tissues (1998)

Modified, human immunodeficiency virus (HIV)-inoculated thy/liv-SCID-hu mice were used to evaluate the in vivo efficacy of antiretroviral drugs. Ritonavir treatment alone initially suppressed plasma viremia, but the viremia recurred with the appearance of ritonavir-resistant HIV isolates. Multidrug therapy suppressed plasma HIV RNA to undetectable levels; however, plasma viremia returned after therapy was stopped, showing that the therapy did not completely suppress HIV infection in the thymic implant. When thy/liv-SCID-hu mice were treated with a combination of zidovudine, lamivudine, and ritonavir immediately after inoculation with HIV, cocultures of the thymic implants remained negative for HIV even 1 month after therapy was discontinued, suggesting that acute treatment can prevent the establishment of HIV infection. Thus, these modified thy/liv-SCID-hu mice should prove to be a useful system for evaluating the effectiveness of different antiretroviral therapies on acute and chronic HIV infection.
How to Assess the Clinical Relevance of Novel RET Missense Variants in the Absence of Functional Studies? (2016)

Our data emphasize the importance of an interdisciplinary approach to assess the functional and clinical significance of novel RET variants. In the absence of functional studies, the plausibility of the pathologic significance of a detected endocrine genetic variant can be estimated by in silico methods such as computational analysis of protein structure and biophysical differences or comparative database search for evolutionary conservation.
Cross Layer Protocols For Multimedia Transmission In Wireless Networks (2012)

Wireless networks initially inherited the traditional layered architecture from wired networks. Nevertheless, as third and fourth generation wireless communications and networking begin to proliferate in the area of communication networks, the suitability of the layered architecture is coming under close scrutiny from the research community. It is repeatedly argued that although layered architectures have served well for wired networks, they might not be suitable for wireless networks One of the key challenges for next-generation broadband wireless networks is to devise end-to-end protocol solutions across wired and wireless networks to accommodate large densities of highly mobile users demanding services and applications with a wide range of Quality of Service (QoS) requirements. Hence, in order to meet the challenging demands on future wireless networks, it may be required to adopt new approaches in which protocols can be designed by violating the reference layered architecture allowing direct communication between protocols in nonadjacent layers Such violations of a layered architecture have been termed as cross-layer design(CLD).
miRanalyzer: an update on the detection and analysis of microRNAs in high-throughput sequencing experiments (2011)

We present a new version of miRanalyzer, a web server and stand-alone tool for the detection of known and prediction of new microRNAs in high-throughput sequencing experiments. The new version has been notably improved regarding speed, scope and available features. Alignments are now based on the ultrafast short-read aligner Bowtie (granting also colour space support, allowing mismatches and improving speed) and 31 genomes, including 6 plant genomes, can now be analysed (previous version contained only 7). Differences between plant and animal microRNAs have been taken into account for the prediction models and differential expression of both, known and predicted microRNAs, between two conditions can be calculated. Additionally, consensus sequences of predicted mature and precursor microRNAs can be obtained from multiple samples, which increases the reliability of the predicted microRNAs. Finally, a stand-alone version of the miRanalyzer that is based on a local and easily customized database is also available; this allows the user to have more control on certain parameters as well as to use specific data such as unpublished assemblies or other libraries that are not available in the web server. miRanalyzer is available at http://bioinfo2.ugr.es/miRanalyzer/miRanalyzer.php.
Percutaneous administration of testosterone gel in postmenopausal women – a pharmacological study (2005)

We wished to investigate if a testosterone gel administered percutaneously to postmenopausal women could result in stable serum levels of the hormone and which dose was required to produce levels within the normal premenopausal range. Fifteen postmenopausal women, mean age 55.3 years (range 45-70 years), volunteered to participate in the study and were divided into three groups. They received 10, 20 or 30 mg of testosterone as a 1% testosterone hydroalcoholic gel at 09.00 hours daily for 14 days. The gel was applied in a thin layer on the outside of the thigh each morning, over an area of approximately 15 cm(2). Blood samples were collected hourly between 09.00 and 17.00 hours on days 1 and 14, and also at 08.00 hours on days 3, 5, 11, 12, 13 and finally day 16, i.e. 2 days after termination of treatment. The mean basal serum level of testosterone was 1.1 +/- 0.9 nmol/l and for 5a-dihydrotestosterone 208 +/- 143 pmol/l. There was a clear increase from the 10 mg to the 20 mg treatment (mean testosterone level during treatment 3.2 and 7.2 nmol/l, respectively) while serum testosterone values after 30 mg showed very little further increase (mean 7.5 nmol/l). Values for days 3-5 were quite similar to those for days 13-14. The present study suggests that adequate and acceptable serum levels of testosterone can be achieved with 10 mg testosterone applied transdermally.
Second-harmonic generation from an array of sub-wavelength metal apertures (2005)

We demonstrate the generation of second-harmonic radiation in transmission through periodic and disordered arrays of sub-wavelength metallic apertures. For circular apertures in a square lattice, the second-harmonic signal peaks at incidence angles corresponding to enhanced transmission of the fundamental beam of 800 nm wavelength except at small incidence angles where the local symmetry minimizes the effective second-order nonlinear susceptibility of the apertures. Even though the linear transmission of the fundamental beam can be more than five times greater through the periodic array as compared to a disordered array, the strength of the second harmonic from the disordered array is greater at large incidence angles. By breaking the local symmetry through the use of apertures of non-centrosymmetric shape, the second-harmonic output occurs at fundamental transmission resonances at small incidence angles.
Mediating Tumor Targeting Efficiency of Nanoparticles Through Design (2009)

Here we systematically examined the effect of nanoparticle size (10−100 nm) and surface chemistry (i.e., poly(ethylene glycol)) on passive targeting of tumors in vivo. We found that the physical and chemical properties of the nanoparticles influenced their pharmacokinetic behavior, which ultimately determined their tumor accumulation capacity. Interestingly, the permeation of nanoparticles within the tumor is highly dependent on the overall size of the nanoparticle, where larger nanoparticles appear to stay near the vasculature while smaller nanoparticles rapidly diffuse throughout the tumor matrix. Our results provide design parameters for engineering nanoparticles for optimized tumor targeting of contrast agents and therapeutics.
Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose (2011)

Lactic acid, an attractive, renewable chemical for production of biobased plastics (polylactic acid, PLA), is currently commercially produced from food-based sources of sugar. Pure optical isomers of lactate needed for PLA are typically produced by microbial fermentation of sugars at temperatures below 40 °C. Bacillus coagulans produces L(+)-lactate as a primary fermentation product and grows optimally at 50 °C and pH 5, conditions that are optimal for activity of commercial fungal cellulases. This strain was engineered to produce D(-)-lactate by deleting the native ldh (L-lactate dehydrogenase) and alsS (acetolactate synthase) genes to impede anaerobic growth, followed by growth-based selection to isolate suppressor mutants that restored growth. One of these, strain QZ19, produced about 90 g L(-1) of optically pure D(-)-lactic acid from glucose in < 48 h. The new source of D-lactate dehydrogenase (D-LDH) activity was identified as a mutated form of glycerol dehydrogenase (GlyDH; D121N and F245S) that was produced at high levels as a result of a third mutation (insertion sequence). Although the native GlyDH had no detectable activity with pyruvate, the mutated GlyDH had a D-LDH specific activity of 0.8 μmoles min(-1) (mg protein)(-1). By using QZ19 for simultaneous saccharification and fermentation of cellulose to D-lactate (50 °C and pH 5.0), the cellulase usage could be reduced to 1/3 that required for equivalent fermentations by mesophilic lactic acid bacteria. Together, the native B. coagulans and the QZ19 derivative can be used to produce either L(+) or D(-) optical isomers of lactic acid (respectively) at high titers and yields from nonfood carbohydrates.
Transport coefficients for stochastic rotation dynamics in three dimensions (2003)

Explicit expressions for the transport coefficients of a recently introduced stochastic model for simulating fluctuating fluid dynamics are derived in three dimensions by means of Green-Kubo relations and simple kinetic arguments. The results are shown to be in excellent agreement with simulation data. Two collision rules are considered and their computational efficiency is compared.
Long-Term Trends in First Hospitalization for Heart Failure and Subsequent Survival Between 1986 and 2003 (2009)

After rising between 1986 and 1994, rates of first hospitalization for HF declined. Case-fatality rates also fell. Prescribing rates for HF therapies increased from 1997 to 2003. These findings suggest that improvements in the prevention and treatment of HF may have had progressive, sustained effects on outcomes at the population level; however, prognosis remains poor in HF.
Arenesulfonate derivatives of homochiral glycidol: versatile chiral building blocks for organic synthesis (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTArenesulfonate derivatives of homochiral glycidol: versatile chiral building blocks for organic synthesisJanice M. Klunder, Tetsuo Onami, and K. Barry SharplessCite this: J. Org. Chem. 1989, 54, 6, 1295–1304Publication Date (Print):March 1, 1989Publication History Published online1 May 2002Published inissue 1 March 1989https://pubs.acs.org/doi/10.1021/jo00267a014https://doi.org/10.1021/jo00267a014research-articleACS PublicationsRequest reuse permissionsArticle Views2252Altmetric-Citations173LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts