gebhart/patrepeval huggingface dataset:
New sunflower and soybean cultivars for novel vegetable oil types (1998)

Commercial production of sunflower and soybean cultivars with altered fatty acid profiles compared to today's commodity oils has been initiated. Sunflower hybrids with oleic contents up to 90% and soybean varieties with less than 4% palmitic acid or less than 3.5% linolenic acid have been developed using mutation breeding. These new oil profiles are available in elite seed products with agronomic performance comparable to that of seeds used for commodity production. These new oil variants have been made without effecting nonacylglycerol components such as tocopherols or phytosterols. Thus, the twofold variation in these minor constituents of oil, that exist in different genotypes of the three major oilseeds, might be exploited to add greater value to novel oils being developed for the oils industry. Genetic variants in fatty acid profile or minor oil constituents, whether developed through mutation breeding or through gene transfer, are providing building blocks to develop an array of new oils for industry. The challenge – and opportunity – is for the oils industry to work with genetic companies in order to create valuable new renewable raw materials that meet the needs of consumers and the general public. Neue Sonnenblumen- und Sojakultivare für neuartige pflanzliche Öle Die kommerzielle Produktion von Sonnenblumen- und Sojabohnenvarietäten mit verändertem Fettsäureprofil, verglichen mit den heutzutage verwendeten Ölrohstoffen, hat begonnen. Sonnenblumenhybride mit Ölsäuregehalten von bis zu 90% und Sojabohnenvarietäten mit weniger als 4% Palmitinsäure oder weniger als 3,5% Linolensäure sind mittels Mutationszüchtung entwickelt worden. Diese neuen Ölprofile sind in ausgelesenen Saatprodukten erhältlich, die eine Agrarleistung mit der von herkömmlicher Saat erlauben. Diese neuen Ölvarietäten wurden hergestellt, ohne einen Einfluß auf Nicht-Acylglycerolkomponenten, wie Tocopherole oder Phytosterole, zu haben. Daher kann die zweifache Veränderung dieser Bestandteile des Öls genutzt werden, um neue Öle noch zu verbessern und für die Ölindustrie zu entwickeln. Genetische Varianten in der Fettsäurezusammensetzung oder in der Zusammensetzung der Ölkomponenten, unabhängig davon, ob sie mittels Mutationszüchtung oder durch Gentransfer entwickelt wurden, stellen ein Terrain mit neuen Wegen für die Ölindustrie dar. Es ist eine Herausforderung und eine Chance für die Ölindustrie, mit Gen-Firmen zusammenzuarbeiten, um hochwertige neue Nachwachsende Rohstoffe zu entwerfen, die den Bedürfnissen der Verbraucher und der allgemeinen Öffentlichkeit entgegenkommen.
Xylanase enzymes promote pulp bleaching (1991)

On passe en revue les possibilites de blanchiment des pâtes kraft a l'aide d'hemicellulases en vue de reduire la consommation de chlore: mecanismes du pretraitement enzymatique, comparaison des enzymes commerciales disponibles, considerations economiques
ECG Manifestations of Myocardial Ischemia (1980)

• Exercise ECG is widely used for the diagnosis of ischemic heart disease. The most common ECG sign of myocardial ischemia is flat or down-sloping ST-segment depression of 1.0 mm or greater. This report draws attention to other much less common, but possibly equally important, ECG manifestations of myocardial ischemia. Serial ECGs obtained during the treadmill stress test of a 40-year-old man with angiographically proven coronary artery disease exhibited virtually all known ECG signs of ischemia, namely, ST-segment depression, ST-segment elevation and alternans, intraventricular conduction abnormalities, and U-wave inversion. (Arch Intern Med 140:1162-1165, 1980)
A parameterized dataflow language extension for embedded streaming systems (2008)

Many embedded DSP systems can be characterized as streaming applications. Imperative programming languages are ill suited for describing the concurrency within these DSP systems. SPEX is a language extension designed to let the programmers describe the inherent parallelism within DSP systems. In this paper, we highlight SPEX’s language features for describing the streaming computation and communication patterns of DSP systems, and allowing the compiler to generate efficient code for embedded DSP architectures. This language extension is based on the parameterized dataflow computation model, with modifications to better describe DSP systems’ complex streaming patterns. SPEX is applied as an extension onto the C++ programming language. It consists of a set of language constructs for describing the semantics of parameterized dataflow computations, and a set of language restrictions for helping the embedded compilation process. In this paper, the W-CDMA wireless protocol is used as our case study.
Genes and Genomes: A Changing Perspective (1990)

Part I: Molecular basis of heredity: An overview: The genetic molecules Replication, maintenance, and modification of the genome The logic and machinery of gene expression Part II: The recombinant DNA breakthrough: The tools: Enzymes The tools: Host-vector systems The means: Constructing, cloning, and selecting recombinant DNA The products: Characterizing and manipulating recombinants Part III: The molecular anatomy, expression and regulation of eukaryotic genes: The structure and regulated expression of eukaryotic genes The molecular anatomy of eukaryotic genomes Genomic rearrangements Part IV: Understanding and manipulating biological systems.
Reporter Mouse Lines for Fluorescence Imaging (2013)

The use of live imaging approaches to examine and understand the dynamic processes that take place during mouse development has become widespread. Several groups have reported their success in generating different reporter mouse lines that express a variety of fluorescent markers for imaging. However, there is currently no established database of the reporter mouse lines available for live imaging, such as the Cre transgenic lines (Cre-X-Mice). Researchers therefore often have difficulties in determining which reporter mouse line meets their research purposes. In this review, we summarize some of the reporter mouse lines that have been generated for live imaging studies, and discuss their characteristics.
SU5416 and SU5614 inhibit kinase activity of wild-type and mutant FLT3 receptor tyrosine kinase (2002)

Internal tandem duplication (ITD) in the juxtamembrane portion of Fms-like tyrosine kinase 3 (FLT3), a type III receptor tyrosine kinase (RTK), is the most common molecular defect associated with acute myeloid leukemia (AML). The high prevalence of this activating mutation makes it a potential target for molecularly based therapy. Indolinone tyrosine kinase inhibitors have known activity against KIT, another member of the type III RTK family. Given the conserved homology between members of this family, we postulated that the activity of some KIT inhibitors would extend to FLT3. We used various leukemic cell lines (BaF3, MV 4-11, RS 4;11) to test the activity of indolinone compounds against the FLT3 kinase activity of both wild-type (WT) and ITD isoforms. Both SU5416 and SU5614 were capable of inhibiting autophosphorylation of ITD and WT FLT3 (SU5416 concentration that inhibits 50% [IC(50)], 100 nM; and SU5614 IC(50) 10 nM). FLT3-dependent activation of the downstream signaling proteins mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 5 (STAT5) was also inhibited by treatment in the same concentration ranges. FLT3 inhibition by SU5416 and SU5614 resulted in reduced proliferation (IC(50), 250 nM and 100 nM, respectively) and induction of apoptosis of FLT3 ITD-positive leukemic cell lines. Treatment of these cells with an alternative growth factor (granulocyte-macrophage colony-stimulating factor [GM-CSF]) restored MAPK signaling and cellular proliferation, demonstrating specificity of the observed inhibitory effects. We conclude that SU5416 and SU5614 are potent inhibitors of FLT3. Our finding that inhibition of FLT3 induces apoptosis of leukemic cells supports the feasibility of targeting FLT3 as a novel treatment strategy for AML.
IL-12 gene therapy protects mice in lethal Klebsiella pneumonia (1996)

IL-12 is a proinflammatory cytokine that has recently been shown to have beneficial effects in the setting of acquired host immunity. To determine the role of IL-12 in innate immunity against Gram-negative bacterial organisms, CBA/J mice were challenged with 10(2) CFU of Klebsiella pneumoniae intratracheally (i.t.), resulting in the time-dependent expression of IL-12 mRNA (p35 and p40) and protein within the lung. Passive immunization of animals with anti-IL-12 serum i.p. at the time of K. pneumoniae inoculation resulted in a 12-fold increase in K. pneumoniae CFU in lung homogenates at 48 h, as compared with animals receiving control serum. In addition, treatment of Klebsiella-infected mice with anti-IL-12 Abs significantly decreased both short and long term survival. To assess the effect of compartmentalized IL-12 overexpression on outcome in Klebsiella pneumonia, animals were treated i.t. with 5 x 10(8) PFU of a nonreplicating adenoviral vector containing a human cytomegalovirus promoter and cDNAs coding for the p35 and p40 subunits of IL-12 inserted into the E1 and E3 domains (Ad5mIL-12), respectively. In vivo transfection with Ad5mIL-12 resulted in 45% long term survival in Klebsiella pneumonia, whereas no animals with Klebsiella pneumonia receiving control adenovirus survived. Moreover, treatment with anti-IFN-gamma Abs or soluble TNF receptor:Ig construct partially and completely attenuated survival benefits observed in animals receiving Ad5mIL-12, respectively. In conclusion, endogenous IL-12 is a critical component of antibacterial host defense, and the compartmentalized overexpression of IL-12 using recombinant adenoviral gene therapy represents a safe and effective approach to deliver IL-12 to the lung in the setting of murine Klebsiella pneumonia.
The use of exome capture RNA-seq for highly degraded RNA with application to clinical cancer sequencing (2015)

RNA-seq by poly(A) selection is currently the most common protocol for whole transcriptome sequencing as it provides a broad, detailed, and accurate view of the RNA landscape. Unfortunately, the utility of poly(A) libraries is greatly limited when the input RNA is degraded, which is the norm for research tissues and clinical samples, especially when specimens are formalin-fixed. To facilitate the use of RNA sequencing beyond cell lines and in the clinical setting, we developed an exome-capture transcriptome protocol with greatly improved performance on degraded RNA. Capture transcriptome libraries enable measuring absolute and differential gene expression, calling genetic variants, and detecting gene fusions. Through validation against gold-standard poly(A) and Ribo-Zero libraries from intact RNA, we show that capture RNA-seq provides accurate and unbiased estimates of RNA abundance, uniform transcript coverage, and broad dynamic range. Unlike poly(A) selection and Ribo-Zero depletion, capture libraries retain these qualities regardless of RNA quality and provide excellent data from clinical specimens including formalin-fixed paraffin-embedded (FFPE) blocks. Systematic improvements across key applications of RNA-seq are shown on a cohort of prostate cancer patients and a set of clinical FFPE samples. Further, we demonstrate the utility of capture RNA-seq libraries in a patient with a highly malignant solitary fibrous tumor (SFT) enrolled in our clinical sequencing program called MI-ONCOSEQ. Capture transcriptome profiling from FFPE revealed two oncogenic fusions: the pathognomonic NAB2-STAT6 inversion and a therapeutically actionable BRAF fusion, which may drive this specific cancer's aggressive phenotype.
Migraine as a Risk Factor for White Matter Lesions, Silent Infarctions, and Ischemic Stroke: The Evidence for a Link (2005)

For decades, migraine has been considered to be an episodic, neurovascular disorder, without long‐term consequences to the brain. Associations between migraine and clinical stroke and migraine and white matter hyperintense lesions have been suggested in numerous studies, but due to various methodological problems no definite conclusion could be drawn from these studies. Recently, data from a population‐based cross‐sectional MRI study in were published. Data from this study established migraine to be a true and independent risk factor for white matter lesions (in female migraine patients) and subclinical posterior circulation territory infarcts. In this review, the methodology and results of previous investigations of a relationship between migraine and clinical ischemic stroke, silent infarction and white matter lesions are reviewed, and integrated in the results from the new MRI study. The conclusion comprises that brain infarction occurs far more frequently than expected in migraine patients, most pronounced in migraine with aura: 8% have subclinical cerebellar infarcts, although most infarcts remain clinically silent. Female migraine patients are at increased risk of deep white matter lesions, independent of the effects of cardiovascular risk factors. The influence of migraine severity (attack frequency) on the risk of both types of lesions suggests a causal relationship between migraine severity and lesion load. Future studies are needed to assess whether these (probably) ischemic lesions have relevant (long‐term) functional correlates.
Preparation of protein conjugates via intermolecular hydrazone linkage (1986)

Proteins can be modified at their amino groups under gentle conditions to contain an average of three to six aryl aldehyde or acyl hydrazide groups. These two types of modified proteins at about 10 microM concentration condense with each other at pH approximately 5 to form conjugates linked by hydrazone bonds. Under proper conditions conjugates mainly of dimers and trimers in size or, if desired, higher oligomers can be obtained. The conjugates can be dissociated to their individual protein components by an exchange reaction with an excess of acetyl hydrazide. The reversible hydrazone bonds of conjugates can be reduced with NaCNBH3 to give stable hydrazide bonds. The stability of protein-hydrazone conjugates was found to be significantly greater than that of the model compound, the N-acetylhydrazone of p-carboxybenzaldehyde. This difference is believed to result from the presence of multiple hydrazone linkages in protein conjugates.
RNase L-Independent Specific 28S rRNA Cleavage in Murine Coronavirus-Infected Cells (2000)

We characterized a novel 28S rRNA cleavage in cells infected with the murine coronavirus mouse hepatitis virus (MHV). The 28S rRNA cleavage occurred as early as 4 h postinfection (p.i.) in MHV-infected DBT cells, with the appearance of subsequent cleavage products and a decrease in the amount of intact 28S rRNA with increasing times of infection; almost all of the intact 28S rRNA disappeared by 24 h p.i. In contrast, no specific 18S rRNA cleavage was detected in infected cells. MHV-induced 28S rRNA cleavage was detected in all MHV-susceptible cell lines and all MHV strains tested. MHV replication was required for the 28S rRNA cleavage, and mature cytoplasmic 28S rRNA underwent cleavage. In certain combination of cells and viruses, pretreatment of virus-infected cells with interferon activates a cellular endoribonuclease, RNase L, that causes rRNA degradation. No interferon was detected in the inoculum used for MHV infection. Addition of anti-interferon antibody to MHV-infected cells did not inhibit 28S rRNA cleavage. Furthermore, 28S rRNA cleavage occurred in an MHV-infected mouse embryonic fibroblast cell line derived from RNase L knockout mice. Thus, MHV-induced 28S rRNA cleavage was independent of the activation of RNase L. MHV-induced 28S rRNA cleavage was also different from apoptosis-related rRNA degradation, which usually occurs concomitantly with DNA fragmentation. In MHV-infected 17Cl-1 cells, 28S rRNA cleavage preceded DNA fragmentation by at least 18 h. Blockage of apoptosis in MHV-infected 17Cl-1 cells by treatment with a caspase inhibitor did not block 28S rRNA cleavage. Furthermore, MHV-induced 28S rRNA cleavage occurred in MHV-infected DBT cells that do not show apoptotic signs, including activation of caspase-3 and DNA fragmentation. Thus, MHV-induced 28S rRNA cleavage appeared to differ from any rRNA degradation mechanism described previously.
MicroRNA-7 targets Nod-like receptor protein 3 inflammasome to modulate neuroinflammation in the pathogenesis of Parkinson’s disease (2016)

Our study provides a direct link between miR-7 and NLRP3 inflammasome-mediated neuroinflammation in the pathogenesis of PD. These findings will give us an insight into the potential of miR-7 and NLRP3 inflammasome in terms of opening up novel therapeutic avenues for PD.
A dose‐response study examining the effects of ritanserin on human slow wave sleep. (1991)

This study investigated the effects of placebo, 1 mg, 3 mg, 10 mg and 30 mg ritanserin and 10 mg diazepam on human sleep. Twelve normal volunteers participated in this randomized, double-blind, placebo-controlled cross-over sleep study. A clear dose-response relationship was found for ritanserin with higher doses evoking increased duration of slow wave sleep.
Predictors of persistent methicillin-resistant Staphylococcus aureus bacteraemia in patients treated with vancomycin (2010)

To minimize the risk of PMRSAB, early removal of implicated devices and evaluation for metastatic infections should be encouraged. Alternative antibiotic therapy is warranted for infections due to isolates with elevated vancomycin MICs, as well as for the high rates of side effects.
Labile iron: manifestations and clinical implications. (2004)

As Dr. Danielson discussed in the article “Structure, Chemistry, and Pharmacokinetics of Intravenous Iron Agents” in this supplement, the pharmacokinetics and internal iron disposition of all intravenous (IV) iron agents are characterized by initial clearance from the plasma space into fixed
Hydrolysis of 3'-terminal mispairs in vitro by the 3' .fwdarw. 5' exonuclease of DNA polymerase .delta. permits subsequent extension by DNA polymerase .alpha. (1990)

Purified DNA polymerase alpha, the major replicating enzyme found in mammalian cells, lacks an associated 3'----5' proofreading exonuclease that, in bacteria, contributes significantly to the accuracy of DNA replication. Calf thymus DNA polymerase alpha cannot remove mispaired 3'-termini, nor can it extend them efficiently. We designed a biochemical assay to search in cell extracts for a putative proofreading exonuclease that might function in concert with DNA polymerase alpha in vivo but dissociates from it during purification. Using this assay, we purified a 3'----5' exonuclease from calf thymus that preferentially hydrolyzes mispaired 3'-termini, permitting subsequent extension of the correctly paired 3'-terminus by DNA polymerase alpha. This exonuclease copurifies with a DNA polymerase activity that is biochemically distinct from DNA polymerase alpha and exhibits characteristics described for a second replicative DNA polymerase, DNA polymerase delta. In related studies, we showed that the 3'----5' exonuclease of authentic DNA polymerase delta, like the purified exonuclease, removes terminal mispairs, allowing extension by DNA polymerase alpha. These data suggest that a single proofreading exonuclease could be shared by DNA polymerases alpha and delta, functioning at the site of DNA replication in mammalian cells.
IMPROMPTU : audio applications for mobile IP (2001)

IMPROMPTU is an Internet Protocol (IF) based audio platfon&amp;apos;n for mobile communications and networked audio applications. IMPROMPTU supports multiple audio applications on a mobile device communicating over the IF network. The audio user interface on the IMPROMPTU client provides speech interfaces to switch applications and control each application with unique vocabulary and grammar along with providing auditory feedback during the usage of the applications. The alerting model is based on the applications competing to attract user&amp;apos;s attention. Various audio applications with different characteristics have been implemented, including radio, MP3 player, and baby monitor. Although IMPROMPTU supports conventional voice telephony services, it does so with increased negotiation between calling parties through more flexible call control. The system architecture is composed of distributed services and applications managed by the Application Manager.
A Novel Fingerprint Scanning Method Using Terahertz Imaging (2018)

In this work., we propose a high spatial resolution terahertz (THz) imaging setup for fingerprint biometrics. THz waves can penetrate into outer drier layers of the skin allowing subsurface imaging for advanced security biometrics or for diagnosis of diabetic diseases. Due to the short wavelengths and significant penetration depth, THz imaging allows to non-invasively probing the finger skin and can provide images of the underlying tissue traits with high spatial resolution. Additionally., THz waves” ability to penetrate thin., non-conductive materials., allows fingerprint scanning even when protective gear is used (e.g. nitrile gloves). In this paper., the theoretical background of the method are discussed along with the proposed imaging configuration. Finally., in-vivo fingerprint images are presented for the first time., establishing the imaging performance of the proposed setup.
Role of phosphatidylinositol 3-kinase-Akt pathway in nucleophosmin/anaplastic lymphoma kinase-mediated lymphomagenesis. (2001)

The NPM/ALK fusion gene, formed by the t(2;5) translocation in a subset of anaplastic large cell lymphomas, encodes a Mr 75,000 hybrid protein that contains the NH2-terminal portion of the nucleolar phosphoprotein nucleophosmin (NPM) joined to the entire cytoplasmic portion of the receptor tyrosine kinase anaplastic lymphoma kinase (ALK). NPM/ALK encodes a constitutively activated tyrosine kinase that belongs to the family of tyrosine kinases activated by chromosomal translocations. Our studies showed that NPM/ALK, similar to other members of this family, activates phosphatidylinositol 3-kinase (PI3K) and its downstream effector, serine/threonine kinase (Akt). PI3K was found in complex with NPM/ALK. Both PI3K and Akt kinase were permanently activated in NPM/ALK-transfected BaF3 murine hematopoietic cells and in NPM/ALK-positive, but not in NPM/ALK-negative, patient-derived anaplastic large cell lymphoma cell lines. In addition, Akt was phosphorylated/activated in protein samples isolated from four patients diagnosed with ALK-positive T/null-cell lymphomas. The PI3K inhibitors wortmannin and LY294002 induced apoptosis in NPM/ALK+ cells but exerted only minor effects on the control BaF3 parental cells and peripheral blood mononuclear cells stimulated by growth factors. Furthermore, retroviral infection of NPM/ALK+ BaF3 cells with a dominant-negative PI3K mutant (delta p85) or a dominant-negative Akt mutant (K179M) inhibited proliferation and clonogenic properties of the infected cells. Finally, the Akt mutant (K179M) suppressed the tumorigenicity of NPM/ALK-transfected BaF3 cells injected into syngeneic mice. In conclusion, our data indicate that NPM/ALK constitutively activates the PI3K-Akt pathway and that this pathway plays an important role in the NPM/ALK-mediated malignant transformation.