gebhart/patrepeval huggingface dataset:
Role of the orphan nuclear receptor RORα in the control of the metastatic behavior of androgen-independent prostate cancer cells (2002)

Orphan nuclear receptors constitute a subgroup of the superfamily of steroid/thyroid/retinoid receptors for which no endogenous ligand has been identified. The orphan nuclear receptor ROR alpha has been shown to be involved in the control of cell growth and differentiation. We have previously shown that, in DU 145 androgen-independent prostate cancer cells, ROR alpha activation brings about a significant decrease of cell proliferation and affects cell cycle progression through the modulation of cell cycle-related genes. The experiments here described have been performed to clarify whether ROR alpha might also be involved in the control of the metastatic behavior of DU 145 cells. We have shown that the thiazolidinedione derivative CGP 52608, the specific ROR alpha ligand and activator, reduces the ability of DU 145 cells to invade a reconstituted basement membrane (Matrigel). CGP 52608 also significantly decreased the capacity of prostate cancer cells to migrate towards a chemotactic stimulus (fibronectin), when plated in the upper compartment of a Boyden's chamber. Moreover, ROR alpha activation resulted in a decreased expression of alpha v beta 3 integrin and an increased level of expression of beta 4 integrin subunit. These findings indicate that the activation of the orphan nuclear receptor ROR alpha reduces the invasive and migratory capacities of androgen-independent prostate cancer cells, at least partially, by affecting integrin expression.
Salivary alpha-amylase and cortisol responsiveness following electrically stimulated physical stress in bipolar disorder patients (2013)

These preliminary results suggest that sAA may be a useful biological marker for BP patients.
Recruiting dendritic cells to improve antibody therapy of cancer (2005)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Real-Time Space Carving Using Graphics Hardware (2007)

Reconstruction of real-world scenes from a set of multiple images is a topic in computer vision and 3D computer graphics with many interesting applications. Attempts have been made to real-time reconstruction on PC cluster systems. While these provide enough performance, they are expensive and less flexible. Approaches that use a GPU hardware-acceleration on single workstations achieve real-time framerates for novel-view synthesis, but do not provide an explicit volumetric representation. This work shows our efforts in developing a GPU hardware-accelerated framework for providing a photo-consistent reconstruction of a dynamic 3D scene. High performance is achieved by employing a shape from silhouette technique in advance. Since the entire processing is done on a single PC, the framework can be applied in mobile environments, enabling a wide range of further applications. We explain our approach using programmable vertex and fragment processors and compare it to highly optimized CPU implementations. We show that the new approach can outperform the latter by more than one magnitude and give an outlook for interesting future enhancements.
Normalized distances for qualitative near-infrared reflectance analysis (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNormalized distances for qualitative near-infrared reflectance analysisHoward. MarkCite this: Anal. Chem. 1986, 58, 2, 379–384Publication Date (Print):February 1, 1986Publication History Published online1 May 2002Published inissue 1 February 1986https://pubs.acs.org/doi/10.1021/ac00293a026https://doi.org/10.1021/ac00293a026research-articleACS PublicationsRequest reuse permissionsArticle Views135Altmetric-Citations46LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
THERMAL PROPERTIES OF SILICA AEROGELS (1989)

The extremely low density of aerogels drastically influences all thermal properties : Compared to vitreous silica, aerogels show enhanced radiative heat transfer ; the solid conduction is reduced by two to three orders of magnitude as can be predicted from the phonon diffusion model ; at low temperatures the specific heat capacity of aerogels is larger by at least a factor of 10 than for non-porous SiO2- glass. Heat transport in monolithic and granular aerogels is discussed with respect to gas pressure, temperature, boundary emissivity and external load.
Reversible brain ischemia: lessons from transient ischemic attack (2007)

Reversible brain ischemia is a harbinger for subsequent ischemic stroke. Although recent advances have focused on imaging markers, the most important predictor of risk following brain ischemia is degree of early clinical reversibility.
Stabilization of the Aggregation of Cyanine Dyes at the Molecular and Nanoscopic Level (2000)

The J-aggregation phenomenon of cyanine dyes has important commercial applications in the photographic industry. A typical cyanine dye was appended to poly-l-lysine in order to stabilize its J-aggregation at the molecular level. Molecular modeling was used to provide information on the conformation of the dye−polymer formed. Possible orientations of poly-l-lysine with cyanine dyes appended to it based on molecular modeling calculations and physical measurements are discussed and evidence for a β-pleated sheet conformation of the poly-l-lysine is shown. Also, for the first time J-aggregated monomer dyes and dye−polymers are used to build layer-by-layer assemblies in combination with inorganic clay sheets. The newly created architectures are used to fabricate three-dimensional structures on a solid support and have potential applications in building layered systems for optical signal processing. Microscopy measurements provide information about the organization of the J-aggregated cyanine dye−clay composites on solid supports.
Metabolic heat production as a measure of macrophage response to particles from orthopedic implant materials (2001)

An in vitro method to gauge metabolic heat response of macrophages (MØ) to particulates is described. Whereas the majority of work cited relies on chemical analysis to assess MØ response to particles, we have used isothermal microcalorimetry (IMC) for direct continuous measurement of metabolic heat production to gauge the response. IMC is a screening method, in that it ensures that no energy-consuming phagocytic response goes undetected, and that the aggregate metabolic magnitude of the responses is determined. A four-well IMC was used in all microcalorimetric measurements. To accommodate "zero-time" monitoring of the interaction of particles and cells, a set of identical test chambers was constructed for use in the IMC. MØs were injected from outside the IMC onto particles contained in collagen or gelatin on glass coverslips at the bottom of each chamber. IMC runs were performed using MØs only, MØs and lipopolysaccharide (LPS) positive control, and MØs and clean or LPS-bound particles of either high-density polyethylene (HDPE) or cobalt-chrome alloy (CoCr). Total heat produced by the negative controls (MØs alone) was lower than for MØ exposure to LPS or particles. The trend was a higher response for LPS-bound HDPE compared with clean HDPE particles, though not significant. In conclusion, our results have shown that IMC can be used to detect the heat associated with the phagocytosis of particulate materials by MØs in vitro.
Identification of Candidate Klf4 Target Genes Reveals the Molecular Basis of the Diverse Regulatory Roles of Klf4 in the Mouse Cornea (2008)

Functionally diverse candidate Klf4 target genes were identified, revealing the molecular basis of the diverse aspects of the Klf4CN corneal phenotype. These results establish Klf4 as an important node in the genetic network of transcription factors regulating the corneal homeostasis.
Neutron dosimetry for a general brachytherapy source (2000)

This paper extends previous work to characterize neutron dosimetry in the vicinity of 252Cf brachytherapy sources. A general source is examined with an arbitrary length, diameter, and encapsulation using Monte Carlo methods. Fast neutron dosimetry and thermal neutron fluence rates were determined in a variety of clinically relevant media of varying dimensions. Applicator Tube, point source, high dose rate VariSource, and high dose rate muSelectron source geometries were analyzed. Fast neutron dosimetry was relatively independent of encapsulation thickness for an assortment of encapsulation materials less than 2 mm thick. Large variations in phantom size made minimal differences in the fast neutron dose close to the source. Specific source geometries were compared with dosimetry obtained from a simplified point model. The consequence of these results is a convenient means of accurately predicting clinical fast neutron dosimetry characteristics around a general 252Cf brachytherapy source in a variety of media without requiring neutron transport. Thermal neutron fluence rates were determined for a variety of source encapsulation materials, encapsulation thicknesses, and phantom sizes. At a distance of 3 cm from the source center, the thermal neutron fluence rate for a 30 cm diameter phantom was a 2.65 times greater than for a 10 cm diameter water phantom. These results demonstrate 252Cf thermal neutron fluence rate is relatively independent of encapsulation thickness and composition, yet highly dependent on hydrogen mass density and phantom size for phanta with diameters <30 cm.
Ffz1, a new transporter specific for fructose from Zygosaccharomyces bailii (2004)

The basis of fructophily in the yeast Zygosaccharomyces bailii has been shown to reside in the performance of transport systems for hexoses. In this study, a gene encoding a fructose-specific transporter was characterized. The strategy involved the functional complementation of a Saccharomyces cerevisiae strain that does not take up hexoses (hxt-null strain). This strain was transformed with a genomic library of Z. bailii. One transformant capable of growing on fructose, but not on glucose, was obtained. This transformant did not transport d-[(14)C]glucose, and the kinetic parameters for d-[(14)C]fructose were V(max)=3.3 mmol h(-1) g(-1) and K(m)=80.4 mM. As in the original strain of Z. bailii, fructose uptake was not inhibited by the presence of other hexoses or uranyl. The plasmid responsible for the observed phenotype was found to carry an ORF encoding a 616 amino acid protein with the characteristics of a membrane transporter, which was designated FFZ1 (fructose facilitator Zygosaccharomyces). The impairment in function observed in an S. cerevisiae transformant expressing a truncated Ffz1 protein lacking 67 amino acids at the C-terminus suggests an important role for this terminal part in the proper structure of the transporter.
Enhancement of PCR amplification yield and specificity using AmpliTaq Gold DNA polymerase. (1998)

Inadequate yields of PCR product and the generation of nonspecific PCR products can complicate genotyping studies, particularly when the DNA template is of inferior quality and/or has a low-copy number. In this study, the ability of AmpliTaq Gold DNA Polymerase to enhance the specificity and yield of amplification was evaluated in a quadruplex short tandem repeat (STR) system in which a nonspecific PCR product and poor yield had been previously observed with AmpliTaq DNA Polymerase usage. Because AmpliTaq Gold is inactive until heated during the PCR before thermal cycling, effects similar to those achieved with "hot-start" PCR were attained in a fast, simple and practical fashion. A significant enhancement in yield at the four STR loci and improved balance of alleles resulted with the use of AmpliTaq Gold. Furthermore, a non-specific PCR product, the result of mispriming, was effectively eliminated. The consistency of quality results was improved, thereby promoting successful typing of suboptimal DNA samples and enhancing the accuracy of genotyping. Since PCR product yield is elevated with AmpliTaq Gold usage, and consistent performance and low background are achieved with higher amounts of AmpliTaq Gold compared with AmpliTaq, AmpliTaq Gold can be used to augment measures taken to counteract the effects of some PCR/Taq DNA polymerase inhibitors, such as those found in blood and some forensic specimens. Studies showed that pH affects either the activity or the activation of the polymerase. AmpliTaq Gold was found to be compatible with pH 8.3 buffers, such as GeneAmp PCR Buffer and AmpFlSTR PCR Reaction Mix but not compatible with pH 9.0 buffers, such as GenePrint STR 10 x Buffer (however, conditions for the usage of AmpliTaq Gold with the GenePrint CTTv system are provided). AmpliTaq Gold is useful for the development and optimization of multiplex amplification systems, particularly those in which the primers are not well designed and/or the reaction conditions are not optimal. Finally, because AmpliTaq Gold is initially inactive, preparation of reactions at ambient temperature and automation of the PCR are facilitated. Therefore throughput can be expanded significantly with the use of AmpliTaq Gold DNA Polymerase.
Improved Cancer Mortality with Low-Molecular-Weight Heparin Treatment: A Review of the Evidence (2002)

Work with low-molecular-weight heparins (LMWHs) continues to provide suggestions for survival advantages among patients with cancer diagnoses. Momentum is building in support of this theory through reports, the vast majority of which are derived from secondary analyses of clinical trials on the treatment of thromboembolism. The data retrieved from such studies that compare unfractionated heparin (UFH) with LMWH indicate that LMWH is equally beneficial if not more beneficial to cancer patients in terms of survival. In retrospective analysis, this improved life expectancy is not considered a result of reduced complications from thromboembolism. Thus, theories of antitumor effects of LMWH have developed, supported by evidence that most of the survival benefits are during long-term comparisons. Reports describing the effects of heparin in the setting of cancer have existed for over a half-century, although specific mechanisms for the marginal results seen thus far have yet to surface. Proposals for the most likely targets of the effective heparins include enzyme interaction, cellular growth modifications, and antiangiogenesis.
Antigenic Relationship between Hantaviruses Analysed by Immunoprecipitation (1988)

Antigenic relationships between seven hantaviruses isolated in Sweden, Belgium, Korea, European U.S.S.R. and Asian U.S.S.R. were studied by radioimmunoprecipitation assays (RIPA) and indirect immunofluorescence tests (IFT). Animal immune sera and haemorrhagic fever with renal syndrome (HFRS) patient sera from the above countries were used. The strains fell into three antigenic groups by both RIPA and IFT: nephropathia epidemica (NE)-type, Korean haemorrhagic fever (KHF)-type and urban rat-type. This antigenic grouping conforms to the clinical grouping of the respective HFRS patients. The major cross-reactive antigen between the antigenic groups was the nucleocapsid protein. Of the two viral glycoproteins, the G2 exhibited weak cross-reactivity between the groups, while the G1 protein appeared to be the least cross-reactive. Patient sera collected at different intervals after the onset of disease showed a marked difference in their capacity to immunoprecipitate the homologous viral glycoproteins, although by IFT all sera had high titres. Host species-related antibody response differences were found by both RIPA and IFT. Using patient sera, a one-way cross-reactivity was seen between the NE-type and the KHF-type viruses. However, animal immune sera clearly demonstrated a reciprocal cross-reactivity between the two virus groups.
Operating stability of light-emitting polymer diodes based on poly(p-phenylene vinylene) (1997)

We have developed modified poly(p-phenylene vinylene) (PPV) to improve its performance in organic electroluminescent devices. Encapsulated single-layer devices with this PPV on indium–tin oxide and a calcium cathode have been operated in air for &amp;gt;7000 h at 20 °C and &amp;gt;1100 h at 80 °C without noticeable degradation. Devices fabricated with this PPV, a conducting polymer layer as anode, and a sputtered low work-function alloy as cathode have efficiencies of over 2 lm/W, brightnesses of &amp;gt;600 Cd/m2 at 4 V, and lifetimes of &amp;gt;1400 h driven at ∼100 Cd/m2 initial brightness.
A Java based implementation of a network level information model for the ATM/frame relay interconnection (2002)

In this paper, we illustrate the use of the Java technology to implement a network information model which captures the essence of ATM and frame relay interconnection. We show how the TMN concepts stay valid and how the existing and extended information models can be reused and implemented according to the TMN principles in a full Java environment.
ANG II-induced cell proliferation is dually mediated by c-Src/Yes/Fyn-regulated ERK1/2 activation in the cytoplasm and PKCζ-controlled ERK1/2 activity within the nucleus (2006)

High-affinity binding of angiotensin II (ANG II) to the ANG II type 1 receptor (AT(1)R) results in the activation of ERK1/2 mitogen-activated protein kinases (MAPK). However, the precise mechanism of ANG II-induced ERK1/2 activation has not been fully characterized. Here, we investigated the signaling events leading to ANG II-induced ERK1/2 activation using a c-Src/Yes/Fyn tyrosine kinase-deficient mouse embryonic fibroblast (MEF) cell line stably transfected with the AT(1)R (SYF/AT(1)). ERK1/2 activation was reduced by approximately 50% within these cells compared with wild-type controls (WT/AT(1)). The remaining approximately 50% of intracellular ERK1/2 activation was dependent upon heterotrimeric G protein and protein kinase C zeta (PKCzeta) activation. Therefore, ANG II-induced ERK1/2 activation occurs via two independent mechanisms. We next investigated whether a loss of either c-Src/Yes/Fyn or PKCzeta signaling affected ERK1/2 nuclear translocation and cell proliferation in response to ANG II. ANG II-induced cell proliferation was markedly reduced in SYF/AT(1) cells compared with WT/AT(1) cells (P < 0.01), but interestingly, ERK2 nuclear translocation was normal. ANG II-induced nuclear translocation of ERK2 was blocked via pretreatment of WT/AT(1) cells with a PKCzeta pseudosubstrate. ANG II-induced cell proliferation was significantly reduced in PKCzeta pseudosubstrate-treated WT/AT(1) cells (P < 0.01) and was completely blocked in SYF/AT(1) cells treated with this same compound. Thus ANG II-induced cell proliferation appears to be regulated by both ERK1/2-driven nuclear and cytoplasmic events. In response to ANG II, the ability of ERK1/2 to remain within the cytoplasm or translocate into the nucleus is controlled by c-Src/Yes/Fyn or heterotrimeric G protein/PKCzeta signaling, respectively.
A 2.7nJ/b multi-standard 2.3/2.4GHz polar transmitter for wireless sensor networks (2012)

This paper presents an ultra-low-power (ULP) 2.3/2.4GHz multi-standard transmitter (TX) for wireless sensor networks and wireless body area networks. Several 2.3/2.4GHz wireless standards have been proposed for such applications, including IEEE802.15.6 (BAN) for body area networks, IEEE802.15.4 (Zigbee) and Bluetooth Low Energy (BLE) for sensor networks and IEEE802.15.4g (SUN) for smart buildings. Recent standard compliant short-range TXs [1-6] typically consume DC power in the range of 20 to 50mW. This is rather high for autonomous systems with limited battery energy. Implemented in a 90nm CMOS technology, the presented TX saves at least 75% of power consumption by replacing several power-hungry analog blocks with the digitally-assisted circuits. This TX is compliant with all 4 of these standards, while dissipating only 4.5mA from a 1.2V supply.
The hormonal action of IGF1 in postnatal mouse growth (2008)

The mammalian insulin-like growth factor 1 (IGF1), which is a member of a major growth-promoting signaling system, is produced by many tissues and functions throughout embryonic and postnatal development in an autocrine/paracrine fashion. In addition to this local action, IGF1 secreted by the liver and circulating in the plasma presumably acts systemically as a classical hormone. However, an endocrine role of IGF1 in growth control was disputed on the basis of the results of a conditional, liver-specific Igf1 gene knockout in mice, which reduced significantly the level of serum IGF1, but did not affect average body weight. Because alternate interpretations of these negative data were tenable, we addressed genetically the question of hormonal IGF1 action by using a positive experimental strategy based on the features of the cre/loxP recombination system. Thus, we generated bitransgenic mice carrying in an Igf1 null background a dormant Igf1 cDNA placed downstream of a transcriptional "stop" DNA sequence flanked by loxP sites (floxed) and also a cre transgene driven by a liver-specific promoter. The Igf1 cDNA, which was inserted by knock-in into the mutated and inactive Igf1 locus itself to ensure proper transcriptional regulation, was conditionally expressed from cognate promoters exclusively in the liver after Cre-mediated excision of the floxed block. Our genetic study demonstrated that the endocrine IGF1 plays a very significant role in mouse growth, as its action contributes approximately30% of the adult body size and sustains postnatal development, including the reproductive functions of both mouse sexes.
Growth of a single freestanding multiwall carbon nanotube on each nanonickel dot (1999)

Patterned growth of freestanding carbon nanotube(s) on submicron nickel dot(s) on silicon has been achieved by plasma-enhanced-hot-filament-chemical-vapor deposition (PE-HF-CVD). A thin film nickel grid was fabricated on a silicon wafer by standard microlithographic techniques, and the PE-HF-CVD was done using acetylene (C2H2) gas as the carbon source and ammonia (NH3) as a catalyst and dilution gas. Well separated, single carbon nanotubes were observed to grow on the grid. The structures had rounded base diameters of approximately 150 nm, heights ranging from 0.1 to 5 μm, and sharp pointed tips. Transmission electron microscopy cross-sectional image clearly showed that the structures are indeed hollow nanotubes. The diameter and height depend on the nickel dot size and growth time, respectively. This nanotube growth process is compatible with silicon integrated circuit processing. Using this method, devices requiring freestanding vertical carbon nanotube(s) such as scanning probe microscopy, field emission flat panel displays, etc. can be fabricated without difficulty.