gebhart/patrepeval huggingface dataset:
Simulink model of flying capacitor multilevel inverter (2008)

In this paper a method for modelling and simulation in Simulink/Matlab environment of the flying capacitor multilevel inverters is presented. The model is used for the harmonic analysis of the output voltage of the inverter when different PWM strategies are utilized. Under investigation are those strategies that solve the capacitor voltage balancing problem: phase-shift PWM method, the saw-tooth rotation PWM method and carrier redistribution PWM method. The results are shown through simulation.
An Improved Protocol for Sequencing of Repetitive Genomic Regions and Structural Variations Using Mutagenesis and Next Generation Sequencing (2012)

The rise of Next Generation Sequencing (NGS) technologies has transformed de novo genome sequencing into an accessible research tool, but obtaining high quality eukaryotic genome assemblies remains a challenge, mostly due to the abundance of repetitive elements. These also make it difficult to study nucleotide polymorphism in repetitive regions, including certain types of structural variations. One solution proposed for resolving such regions is Sequence Assembly aided by Mutagenesis (SAM), which relies on the fact that introducing enough random mutations breaks the repetitive structure, making assembly possible. Sequencing many different mutated copies permits the sequence of the repetitive region to be inferred by consensus methods. However, this approach relies on molecular cloning in order to isolate and amplify individual mutant copies, making it hard to scale-up the approach for use in conjunction with high-throughput sequencing technologies. To address this problem, we propose NG-SAM, a modified version of the SAM protocol that relies on PCR and dilution steps only, coupled to a NGS workflow. NG-SAM therefore has the potential to be scaled-up, e.g. using emerging microfluidics technologies. We built a realistic simulation pipeline to study the feasibility of NG-SAM, and our results suggest that under appropriate experimental conditions the approach might be successfully put into practice. Moreover, our simulations suggest that NG-SAM is capable of reconstructing robustly a wide range of potential target sequences of varying lengths and repetitive structures.
Organic complementary ring oscillators (1999)

We report the characteristics of complementary organic ring oscillators. The shortest delay time measured is 38 μs per stage that corresponds to a 2.63 kHz oscillation frequency. The active material in the n-channel transistors is copper hexadecafluorophthalocyanine and that in the p-channel transistors is an oligothiophene/oligothiophene derivative.
The Pattern Recognition Basis of Artificial Intelligence (1998)

From the Publisher:
This book pays extra attention to the new ideas in AI: neural networking, case based reasoning, and memory based reasoning, while including the important aspects of traditional symbol processing AI. As much as possible, these methods are compared with each other so that the reader will see the advantages and disadvantages of each method. Second, the new and traditional methods are presented as different ways of doing pattern recognition, giving unity to the subject matter. Third, rather than treating AI as just a collection of advanced algorithms, it also looks at the problems involved in producing the kind of general purpose intelligence found in human beings who have to deal with the real world.
An Antibody-Avidin Fusion Protein Specific for the Transferrin Receptor Serves as a Delivery Vehicle for Effective Brain Targeting: Initial Applications in Anti-HIV Antisense Drug Delivery to the Brain (1999)

In the present study a novel Ab-avidin fusion protein has been constructed to deliver biotinylated compounds across the blood brain barrier. This fusion molecule consists of an Ab specific for the transferrin receptor genetically fused to avidin. The Ab-avidin fusion protein (anti-TfR IgG3-CH3-Av) expressed in murine myeloma cells was correctly assembled and secreted and showed both Ab- and avidin-related activities. In animal models, it showed much longer serum half-life than the chemical conjugate between OX-26 and avidin. Most importantly, this fusion protein demonstrated superior [3H]biotin uptake into brain parenchyma in comparison with the chemical conjugate. We also delivered a biotinylated 18-mer antisense peptide-nucleic acid specific for the rev gene of HIV-1 to the brain. Brain uptake of the HIV antisense drug was increased at least 15-fold when it was bound to the anti-TfR IgG3-CH3-Av, suggesting its potential use in neurologic AIDS. This novel Ab fusion protein should have general utility as a universal vehicle to effectively deliver biotinylated compounds across the blood-brain barrier for diagnosis and/or therapy of a broad range of CNS disorders such as infectious diseases, brain tumors as well as Parkinson's and Huntington's diseases.
Dependence of temperature and strain coefficients on fiber grating type and its application to simultaneous temperature and strain measurement (2002)

We report an investigation of the dependence of the temperature and strain coefficients on the grating type for fiber Bragg gratings that are UV inscribed in B/Ge-codoped fiber with and without hydrogenation. The results reveal that all types of grating exhibit similar strain sensitivities but markedly different temperature sensitivities, greater for gratings inscribed in hydrogen-free rather than hydrogenated fiber and substantially less in type IA gratings than all others. The sensitivity characteristics of these gratings have been used to implement a new type of dual-grating sensor for simultaneous measurement of temperature and strain that has properties superior to those of previously reported structures.
Nanopatterning proteins and peptides (2006)

A variety of techniques have been developed to site-specifically immobilize biomolecules onto surfaces with resolutions below one micron. The ability to pattern proteins and peptides in particular has great potential for applications in biosensors, biomaterials, and tissue engineering. For example, immobilizing proteins at the nanoscale could lead to the development of diagnostic protein nanoarrays, while patterning peptides could lead to greater control over the cell/biomaterial interface. This review discusses the methods that have been reported for patterning proteins and peptides with submicron and nanometer resolutions.
A theory of type qualifiers (1999)

We describe a framework for adding type qualifiers to a language. Type qualifiers encode a simple but highly useful form of subtyping. Our framework extends standard type rules to model the flow of qualifiers through a program, where each qualifier or set of qualifiers comes with additional rules that capture its semantics. Our framework allows types to be polymorphic in the type qualifiers. We present a const-inference system for C as an example application of the framework. We show that for a set of real C programs, many more consts can be used than are actually present in the original code.
The effect of cyclic AMP on the rabbit corneal endothelial fluid pump (1982)

Perfusing preswollen deepithelialized rabbit cornea in vitro on its endothelial side with balanced salt solution containing physiological glucose concentration and 10(-6)M adenosine, we can obtain "temperature reversal" from 500-520 microns to less than 400 microns, during 7 hours. Low concentrations of adenosine improve the fluid pump supposedly by inhibiting adenylate cyclase, since c-AMP inhibits the pump.
The Military Defeat of the South Africans in Angola (1989)

Ten years after United Nations Resolution 435 laid the basis for an independent Namibia, the South Africans agreed to withdraw from the territory they still occupied in defiance of international opinion. In a ceremony at U.N. headquarters in New York on December 22, 1988, an agreement was signed by Angola, Cuba, and South Africa, with the United States ostensibly acting as mediator. This accord was a major step toward self-determination for the peoples of Southern Africa, for it finally gave the United Nations Transitional Group the go-ahead to implement steps for the withdrawal of South African troops from Namibia and the return of refugees, elections, and independence to the former Portuguese colony. This historic agreement came not because of the tenacious negotiating of U.S. Assistant Secretary of State for African Affairs Chester Crocker, but because of the decisive military defeat of the South African forces at Cuito Cuanavale in Angola.This article can also be found at the Monthly Review website, where most recent articles are published in full.Click here to purchase a PDF version of this article at the Monthly Review website.
BRAF V600E Maintains Proliferation, Transformation, and Tumorigenicity of BRAF-Mutant Papillary Thyroid Cancer Cells (2007)

BRAF V600E is not only an initiator of PTC as demonstrated previously but is also a maintainer of proliferation, transformation, and tumorigenicity of PTC cells harboring BRAF mutation, and growth of tumors derived from such cells continues to depend on BRAF V600E. These results provide further support for potentially effective therapy targeted at BRAF for BRAF mutation-harboring PTC.
High-Density Lipoprotein Modulates Glucose Metabolism in Patients With Type 2 Diabetes Mellitus (2009)

rHDL reduced plasma glucose in patients with type 2 diabetes mellitus by increasing plasma insulin and activating AMP-activated protein kinase in skeletal muscle. These findings suggest a role for HDL-raising therapies beyond atherosclerosis to address type 2 diabetes mellitus.
Variable-Region-Identical Antibodies Differing in Isotype Demonstrate Differences in Fine Specificity and Idiotype (2005)

A central tenet of the current understanding of the relationship between Ab structure and function is that the variable region domain is solely responsible for Ag specificity. However, this view was recently challenged by the observation that families of mouse-human chimeric Abs with identical V regions demonstrate differences in fine specificity and by reports of changes in Ab Id structure with isotype switching. Here we revisited this question by evaluating the reactivity of two families of murine IgG switch variants that differed in V region usage for Cryptococcus neoformans glucuronoxylomannan, glucuronoxylomannan peptide mimetics, and anti-Id mAbs. The results reveal isotype-related differences in fine specificities and Id for two mAb isotype switched families, thus establishing the validity of this observation with sets of homologous Abs. The results suggest that the C region affects V region protein conformation, leading to differences in fine specificity and Id. The finding that isotype can affect fine specificity has major implications for current concepts of the generation of secondary responses, idiotypic network regulation, and isotype function. Given that isotype class switching and Ig gene somatic hypermutation share molecular mechanisms, these observations unify these processes in the sense that both can alter specificity and affinity.
An antibody which binds to the membrane-proximal end of influenza virus haemagglutinin (H3 subtype) inhibits the low-pH-induced conformational change and cell-cell fusion but does not neutralize virus. (1998)

A monoclonal antibody, LMBH6, was derived from mice which had been sequentially immunized with bromelain-cleaved haemagglutinin (BHA) from influenza virus A/Aichi/2/68, A/Victoria/3/75 and A/Philippines/2/82 (all H3N2). LMBH6 recognizes the haemagglutinin (HA) of all H3N2 influenza A strains tested, which were isolated between 1968 and 1989. HA in the low-pH-induced conformation is not recognized, and cleavage of the HA0 precursor to HA1 and HA2 is needed to obtain efficient binding. Compared to other monoclonal antibodies, binding of LMBH6 to virus and to virus-infected cells is weak, while binding to BHA is comparable. Electron microscopy demonstrates binding to the membrane proximal end of the stem structure. The antibody shows no haemagglutination-inhibition activity, but inhibits polykaryon formation and the low-pH-induced conformational change of BHA. However, LMBH6 cannot prevent infection of MDCK cells but slows the growth of virus when included in a plaque assay overlay.
Analysis of structural polypeptides of purified human cytomegalovirus (1976)

Human cytomegalovirus strain C87 was purified by the following procedures. (i) Extracellular virus was concentrated by centrifugation at 100,000 X g for 90 min and passed through a Bio-Rad Bio-Gel A-15m column. Most of the virus was recovered in the void volume. (ii) After two consecutive isopycnic potassium tartrate gradient centrifugations (20 to 50%), coinciding peaks of plaque titer, protein, and radioactivity were found at a density of from 1.20 to 1.21 g/cm3. To characterize the structural polypeptides of human cytomegalovirus and to establish relative purification criteria, virus was purified from two mixtures: (i) [35S]methionine-labeled extracellular virus mixed with an equal volume of unlabeled normal culture fluid; (ii) unlabeled extracellular virus mixed with an equal volume of [357a1methionine-labeled normal culture fluid. The extent of purification, as judged by the ratio of cellular to viral radioactivity, was 39-fold; i.e. about 2.5% of the protein in the purified virus preparation could be accounted for by host protein contamination. Electrophoresis of purified [35S]methionine-labeled virus on a polyacrylamide gel slab showed that there were at least 33 viral structural polypeptides (VPs), and their molecular weights ranged from 11,000 to 290,000. Autoradiograms obtained from electropherograms of purified [14C]glucosamine labeled virus showed six bands. Four of these were so broad that several VPs corresponded to each of the glycosylated bands. When heavy (two fractions close to 1.21 g/cm3) and light (two fractions close to 1.20 g/cm3) fractions of the PFU peak from the second potassium tartrate gradient were analyzed separately, the number of polypeptides observed was the same, but the relative amounts of some polypeptides differed. The major polypeptide, VP17, was found in greater amounts in the heavy fraction (35%) than in the light fraction (22%). The amount of DNA as a percentage of the weight of protein was 2% for the light fraction and 1% for the heavy fraction.
Design and synthesis of potential DNA cross-linking reagents based on the anthramycin class of minor groove binding compounds (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDesign and synthesis of potential DNA cross-linking reagents based on the anthramycin class of minor groove binding compoundsPat N. Confalone, Edward M. Huie, Soo S. Ko, and Greg M. ColeCite this: J. Org. Chem. 1988, 53, 3, 482–487Publication Date (Print):February 1, 1988Publication History Published online1 May 2002Published inissue 1 February 1988https://pubs.acs.org/doi/10.1021/jo00238a004https://doi.org/10.1021/jo00238a004research-articleACS PublicationsRequest reuse permissionsArticle Views1783Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Gate-Controlled Microfluidic Chamber With Magnetic Bead for DNA Sequencing-by-Synthesis Technology (2007)

In this paper we present a novel microfluidic platform for DNA sequencing-by-synthesis methods (e.g. pyrosequencing). The proposed platform is based on the valve-controllable PDMS channel technology with DNA-coated magnetic beads. The encapsulation of the reaction of DNA polymerization in picoliter-sized wells provides for excellent isolation and control for detection. This separation prevents cross-talk amongst neighbor reactors which is one of the most limitations for higher integration of the current technologies. Through application of an external magnetic field the beads can be allocated with better accuracy. In addition this property can help mixing for the reaction. The proposed system is useful for a number of other bio-species detection and sorting templates. This paper illustrates the design and experimental results of a primary template as well as different advantages and potential applications of the Gate-Controlled Magnetic Bead (GCMB) platform in the world of DNA sequencing and genetics.
Fidelity of seryl-tRNA synthetase to binding of natural amino acids from HierDock first principles computations (2006)

Seryl-tRNA synthetase (SerRS) charges serine to tRNA(Ser) following the formation of a seryl adenylate intermediate, but the extent to which other non-cognate amino acids compete with serine to bind to SerRS or for the formation of the activated seryl adenylate intermediate is not known. To examine the mechanism of discrimination against non-cognate amino acids, we calculated the relative binding energies of the 20 natural amino acids to SerRS. Starting with the crystal structure of SerRS from Thermus thermophilus with seryl adenylate bound, we used the HierDock and SCREAM (Side-Chain Rotamer Energy Analysis Method) computational methods to predict the binding conformation and binding energy of each of the 20 natural amino acids in the binding site in the best-binding mode and the activating mode. The ordering of the calculated binding energies in the activated mode agrees with kinetic measurements in yeast SerRS that threonine will compete with serine for formation of the activated intermediate while alanine and glycine will not compete significantly. In addition, we predict that asparagine will compete with serine for formation of the activated intermediate. Experiments to check the accuracy of this prediction would be useful in further validating the use of HierDock and SCREAM for designing novel amino acids to incorporate into proteins and for determining mutations in aminoacyl-tRNA synthetase design to facilitate the incorporation of amino acid analogs into proteins.
Quantitative Computed Tomography of the Lungs and Airways in Healthy Nonsmoking Adults (2012)

Although the attenuation characteristics of normal lung differ by age and sex, these differences do not persist on multivariate analysis. Potential sources of variation in measurement of attenuation-based QCT parameters include depth of inspiration/expiration and scanner type. Tracheal air attenuation may partially correct variation because of scanner type. Sources of variation in QCT airway measurements may include age, sex, BMI, depth of inspiration, and spatial resolution.
The role of proinflammatory cytokines in the generation and maintenance of joint pain (2010)

The proinflammatory cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) not only promote and maintain inflammation, they also contribute to the generation and maintenance of inflammatory pain by acting at nociceptive nerve cells. A large proportion of dorsal root ganglion (DRG) neurons express TNF receptors and receptor units for stimulation with IL-6. In the rat model of antigen-induced arthritis (AIA), neutralization of TNF-alpha by etanercept and infliximab reduced inflammation-evoked mechanical hyperalgesia at the inflamed knee joint. This treatment also attenuated the infiltration of macrophages into the DRGs usually observed during the acute phase of AIA. Intra-articular application of etanercept reduced the responses of C-fibers to mechanical stimulation of the inflamed joint but did not influence responses to stimulation of the normal joint. Finally, in cultured DRG neurons TNF-alpha increased the proportion of neurons that express the TRPV1 receptor and may thus contribute to the generation of inflammation-evoked thermal hyperalgesia.
Electrode-mediated Wacker oxidation of cyclic and internal olefins (1992)

An improved method for the electrode-mediated oxidations of olefins by palladium(II) is described. Current efficiencies from 80% to 95% were obtained in oxidations of 1-decene, styrene, trans-2-octene, and cyclohexene in which perchloric acid was added to a chloride-free solution of a palladium(II) acetate catalyst. The palladium(0) was reoxidized to palladium(II) by reaction with catalytic amounts of benzoquinone, which was, in turn, regenerated by anodic oxidation. Addition of varying amounts of perchloric acid did not affect the current efficiency but accelerated the oxidation reaction, up to a concentration of approximately 0.15 M. The current efficiency remained high (&gt;80%) over the course of the electrode-mediated oxidations of 1-decene, trans-2-octene, and cyclohexene. At the end of the reactions, when the substrate was depleted, a drastic decrease in the current was observed, indicating that the catalytic cycle leading to product was primarily responsible for the electrochemical reaction. It also was shown that the rates of the electrochemical reactions were generally slower than those of homogeneous reactions in which a stoichiometric amount of benzoquinone was used, indicating that the electrochemical regeneration of benzoquinone was mass transport limited at the highest concentrations of perchloric acid. This is in contrast to other reports in the literature that suggested that the homogeneous (non-electrochemical) reactions were actually slower. Reasons for the discrepancy between these results are discussed.
Spectrophotometric Results from the Copernicus Satellite. I. Instrumentation and Performance (1973)

view Abstract Citations (298) References (7) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS Spectrophotometric Results from the Copernicus Satellite. I. Instrumentation and Performance Rogerson, J. B. ; Spitzer, L. ; Drake, J. F. ; Dressler, K. ; Jenkins, E. B. ; Morton, D. C. ; York, D. G. Abstract The Princeton telescope-spectrometer on the OAO spacecraft Copernicus scans stellar spectra with a resolution of about 0.05 A between 950 and 1450 A, and twice this in first order between 1650 and 3000A. The pointing during several minutes is steady within 0'!02, and the measured photometric precision in the shorter wavelength range is limited only by the statistics of photon counts, with 14-s counts of about 10 on an unreddened 131 star, mv = 5.0, at 1100 A. In the 165030(X) A wavelength range, phototube noise resulting from cosmic rays makes observations difficult on stars fainter than m = 3.0. Subject headings:instruments - spectra, ultraviolet - spectrophotometry - ultraviolet Publication: The Astrophysical Journal Pub Date: May 1973 DOI: 10.1086/181194 Bibcode: 1973ApJ...181L..97R full text sources ADS | Related Materials (5) Part 2: 1973ApJ...181L.103M Part 3: 1973ApJ...181L.110R Part 4: 1973ApJ...181L.116S Part 5: 1973ApJ...181L.122J Part 6: 1973ApJ...182L...1Y