gebhart/patrepeval huggingface dataset:
The Recycling of Apolipoprotein E in Primary Cultures of Mouse Hepatocytes (2003)

Internalization of apoE-containing very low density protein (VLDL) by hepatocytes <i>in vivo</i> and <i>in vitro</i> leads to apoE recycling and resecretion. Because of the role of apoE in VLDL metabolism, apoE recycling may influence lipoprotein assembly or remnant uptake. However, apoE is also a HDL protein, and apoE recycling may be related to reverse cholesterol transport. To investigate apoE recycling, apoE<sup>−/−</sup> mouse hepatocytes were incubated (pulsed) with wild-type mouse lipoproteins, and cells and media were collected at chase periods up to 24 h. When cells were pulsed with VLDL, apoE was resecreted within 30 min. Although the mass of apoE in the media decreased with time, it could be detected up to 24 h after the pulse. Intact intracellular apoE was also detectable 24 h after the pulse. ApoE was also resecreted when cells were pulsed with HDL. When apoA-I was included in the chase media after a pulse with VLDL, apoE resecretion increased 4-fold. Furthermore, human apoE was resecreted from wild-type mouse hepatocytes after a pulse with human VLDL. Finally, apoE was resecreted from mouse peritoneal macrophages after pulsing with VLDL. We conclude that 1) HDL apoE recycles in a quantitatively comparable fashion to VLDL apoE; 2) apoE recycling can be modulated by extracellular apoA-I but is not affected by endogenous apoE; and 3) recycling occurs in macrophages as well as in hepatocytes, suggesting that the process is not cell-specific.
Synthesis, Characterization, and Catalytic Properties of Novel Single-Site and Nanosized Platinum Catalysts (2012)

Novel single-site platinum catalysts have been synthesized by reacting platinum(II) organometallics with partially dehydroxylated silica. The resulting materials have been characterized by various methods such as IR, MAS NMR, and EXAFS. Further, the single-site platinum catalysts were calcined in air to remove the ligand and produce nanosized platinum particles, that were characterized by TEM and H2 chemisorption. All catalysts were tested for the hydrogenation of toluene. The single-site platinum catalysts were less active than a commercial Pt/SiO2 catalyst with comparable platinum loading, and this has been ascribed to ligand effects. Conversely, the nanosized platinum catalysts were more active than the commercial Pt/SiO2 catalyst due to their high dispersion and small particle sizes.
Single-chain antigen recognition receptors that costimulate potent rejection of established experimental tumors (2002)

Tumor cells are usually weakly immunogenic as they largely express self-antigens and can down-regulate major histocompatability complex/peptide molecules and critical costimulatory ligands. The challenge for immunotherapies has been to provide vigorous immune effector cells that circumvent these tumor escape mechanisms and eradicate established tumors. One promising approach is to engineer T cells with single-chain antibody receptors, and since T cells require 2 distinct signals for optimal activation, we have compared the therapeutic efficacy of erbB2-reactive chimeric receptors that contain either T-cell receptor zeta (TCR-zeta) or CD28/TCR-zeta signaling domains. We have demonstrated that primary mouse CD8(+) T lymphocytes expressing the single-chain Fv (scFv)-CD28-zeta receptor have a greater capacity to secrete Tc1 cytokines, induce T-cell proliferation, and inhibit established tumor growth and metastases in vivo. The suppression of established tumor burden by cytotoxic T cells expressing the CD28/TCR-zeta chimera was critically dependent upon their interferon gamma (IFN-gamma) secretion. Our study has illustrated the practical advantage of engineering a T-cell signaling complex that codelivers CD28 activation, dependent only upon the tumor's expression of the appropriate tumor associated antigen.
Pyranmycins, a Novel Class of Aminoglycosides with Improved Acid Stability:  The SAR of d-Pyranoses on Ring III of Pyranmycin (2002)

[reaction: see text] The synthesis of a novel class of aminoglycosides, pyranmycins, is reported along with the structure activity relationship (SAR) of their antibacterial activity against Escherichia coli. Two pyranmycins show prominent activity (9 microM). Pyranmycins also manifest superior stability in acidic media. The SAR information will lead to the future designs of pyranmycin against drug resistant bacteria.
Perinatal listeriosis (early-onset): correlation of antenatal manifestations and neonatal outcome. (1986)

Listeria monocytogenes is an underdiagnosed and underreported cause of congenital sepsis. Twenty mother/infant pairs from whom Listeria was isolated were studied at the University of Southern California School of Medicine and Women's Hospital during the last ten years to delineate antepartum factors indicative of a fetus at high risk for perinatal Listeria sepsis. The combination of high maternal leukocyte count, fetal tachycardia, decreased fetal heart rate variability, and, especially, the absence of intrapartum fetal heart rate accelerations was associated with a complicated course for the neonate with congenital Listeria sepsis. Intrapartum administration of antibiotics decreased fetal morbidity and mortality but did not impair recovery of the organism.
Deep Reinforcement Learning (2019)

We discuss deep reinforcement learning in an overview style.We draw a big picture, filled with details.We discuss six core elements, six important mechanisms, and twelve applications, focusing on contemporary work, and in historical contexts.We start with background of artificial intelligence, machine learning, deep learning, and reinforcement learning (RL), with resources.Next we discuss RL core elements, including value function, policy, reward, model, exploration vs. exploitation, and representation.Then we discuss important mechanisms for RL, including attention and memory, unsupervised learning, hierarchical RL, multiagent RL, relational RL, and learning to learn.After that, we discuss RL applications, including games, robotics, natural language processing (NLP), computer vision, finance,
Nowcasting and Predicting Data Revisions in Real Time Using Qualitative Panel Survey Data (2007)

The qualitative responses that firms give to business survey questions regarding changes in their own output provide a real-time signal of official output changes. The most commonly-used method to produce an aggregate quantitative indicator from business survey responses - the net balance, or diffusion index - has changed little in 40 years. It focuses on the proportion of survey respondents replying "up", "the same" or "down". This paper investigates whether an improved real-time signal of official output data changes can be derived from a recently advanced method on the aggregation of survey data from panel responses. It also considers the ability of survey data to anticipate revisions to official output data. We find, in a New Zealand application, that exploiting the panel dimension to qualitative survey data gives a better in-sample signal about official data than traditional methods. This is achieved by giving a higher weight to firms whose answers have a close link to official data than to those whose experiences correspond only weakly or not at all. Out-of-sample, it is less clear it matters how survey data are quantified with simpler and more parsimonious methods hard to improve. It is clear, nevertheless, that survey data, exploited in some form, help to explain revisions to official data.
Shared susceptibility for celiac disease and inflammatory bowel disease? (2008)

To the Editor: We read with interest the study by Leeds et al. assessing the relationship between celiac disease and inflammatory bowel disease [1]. In their investigation, they found an increased ...
Synthesis and Biological Evaluation of Certain α,β-Unsaturated Ketones and Their Corresponding Fused Pyridines as Antiviral and Cytotoxic Agents (2000)

A new series of 3,5-bis(arylidene)-4-piperidones, as chalcone analogues carrying variety of aryl and heteroaryl groups, pyrazolo[4,3-c]pyridines, pyrido[4,3-d]pyrimidines, and pyrido[3,2-c]pyridines, carrying an arylidene moiety, and a series of pyrano[3,2-c]pyridines, as flavone and coumarin isosteres, were synthesized and screened for their in vitro antiviral and antitumor activities at the National Cancer Institute (NCI). Compounds 9 and 18 proved to be active against herpes simplex virus-1 (HSV-1), while compound 13 showed moderate activity against human immunodeficiency virus-1 (HIV-1). Compounds 14, 26, 28, 33, and 35 exhibited a broad spectrum antitumor activity. In addition, compounds 26, 33, and 35 proved to be of moderate selectivity toward leukemia cell lines. The pyrano[3,2-c]pyridines heterocyclic system proved to be the most active antitumors among the investigated heterocycles.
Allografting Intervertebral Discs in Dogs (1996)

Our results indicate that allograft of intervertebral discs has clinical potential because it can serve as a dynamic disc spacer for a certain period of time, but its long-term merits and demerits have to be established.
Vaccination with aShigellaDNA Vaccine Vector Induces Antigen-Specific CD8+T Cells and Antiviral Protective Immunity (2001)

A prototype Shigella human immunodeficiency virus type 1 (HIV-1) gp120 DNA vaccine vector was constructed and evaluated for immunogenicity in a murine model. For comparative purposes, mice were also vaccinated with a vaccinia virus-env (vaccinia-env) vector or the gp120 DNA vaccine alone. Enumeration of the CD8(+)-T-cell responses to gp120 after vaccination using a gamma interferon enzyme-linked spot assay revealed that a single intranasal dose of the Shigella HIV-1 gp120 DNA vaccine vector elicited a CD8(+) T-cell response to gp120, the magnitude of which was comparable to the sizes of the analogous responses to gp120 that developed in mice vaccinated intraperitoneally with the vaccinia-env vector or intramuscularly with the gp120 DNA vaccine. In addition, a single dose of the Shigella gp120 DNA vaccine vector afforded significant protection against a vaccinia-env challenge. Moreover, the number of vaccinia-env PFU recovered in mice vaccinated intranasally with the Shigella vector was about fivefold less than the number recovered from mice vaccinated intramuscularly with the gp120 DNA vaccine. Since the Shigella vector did not express detectable levels of gp120, this report confirms that Shigella vectors are capable of delivering passenger DNA vaccines to host cells and inducing robust CD8(+) T-cell responses to antigens expressed by the DNA vaccines. Furthermore, to our knowledge, this is the first documentation of antiviral protective immunity following vaccination with a live Shigella DNA vaccine vector.
A Prospective Study of Dairy Intake and the Risk of Type 2 Diabetes in Women (2006)

A dietary pattern that incorporates higher low-fat dairy products may lower the risk of type 2 diabetes in middle-aged or older women.
Quantitative analysis of living cells by digital holographic microscopy (2009)

Digital holography is applied to obtain noninvasive quantitative phase measurements from living cells. The complex wavefront can be recorded in just a single digital image allowing for critical real-time imaging. The vertical resolution of measurements is determined to be around 3 nanometers.
Resveratrol induces apoptosis in LNCaP cells and requires hydroxyl groups to decrease viability in LNCaP and DU 145 cells (2002)

Resveratrol was toxic to cells regardless of whether the cells were hormone-responsive or -unresponsive. This finding suggests that the cell's hormone responsive status is not an important determinant of the response to resveratrol. Furthermore, the hydroxyl-groups on resveratrol are required for cell toxicity. Finally resveratrol but not DES induced caspase-mediated apoptosis.
Wearable Sensor Use for Assessing Standing Balance and Walking Stability in People with Parkinson’s Disease: A Systematic Review (2015)

CRD42014010838.
Venezuelan Equine Encephalitis Virus Replicon Particles Encoding Respiratory Syncytial Virus Surface Glycoproteins Induce Protective Mucosal Responses in Mice and Cotton Rats (2007)

Respiratory syncytial virus (RSV) is an important viral pathogen that causes severe lower respiratory tract infection in infants, the elderly, and immunocompromised individuals. There are no licensed RSV vaccines to date. To prevent RSV infection, immune responses in both the upper and lower respiratory tracts are required. Previously, immunization with Venezuelan equine encephalitis virus replicon particles (VRPs) demonstrated effectiveness in inducing mucosal protection against various pathogens. In this study, we developed VRPs encoding RSV fusion (F) or attachment (G) glycoproteins and evaluated the immunogenicity and efficacy of these vaccine candidates in mice and cotton rats. VRPs, when administered intranasally, induced surface glycoprotein-specific virus neutralizing antibodies in serum and immunoglobulin A (IgA) antibodies in secretions at the respiratory mucosa. In addition, fusion protein-encoding VRPs induced gamma interferon (IFN-gamma)-secreting T cells in the lungs and spleen, as measured by reaction with an H-2K(d)-restricted CD8(+) T-cell epitope. In animals vaccinated with F protein VRPs, challenge virus replication was reduced below the level of detection in both the upper and lower respiratory tracts following intranasal RSV challenge, while in those vaccinated with G protein VRPs, challenge virus was detected in the upper but not the lower respiratory tract. Close examination of histopathology of the lungs of vaccinated animals following RSV challenge revealed no enhanced inflammation. Immunization with VRPs induced balanced Th1/Th2 immune responses, as measured by the cytokine profile in the lungs and antibody isotype of the humoral immune response. These results represent an important first step toward the use of VRPs encoding RSV proteins as a prophylactic vaccine for RSV.
Increased risk of multiple sclerosis after late Epstein-Barr virus infection: a historical prospective study (1995)

An association between infectious mononucleosis (IM) and MS has been proposed. In a historical prospective study we used records from the Danish State Serum Institute on heterophile antibody (HA) tests for IM performed in all Danish patients over a number of years. Included in the analysis were 6853 HA-positive persons analyzed from 1968 to 1978 (except 1975) and 12,886 HA-negative per sons analyzed in the years 1968, 1969, 1970 and 1978. A search for these persons in the central nationwide Danish Multiple Sclerosis Registry (DMSR) was performed. Among the HA-positive persons 16 cases of MS which met the diagnostic criteria were found with onset of MS after the year of the HA test and before follow-up on 1 January 1991. The expected number for a Danish population, matched by sex, age and year at start of observation, was 5.70 (P < 0.05), the risk ratio being 2.81. No patient had developed MS before contracting IM. Among the HA-negative persons 12 were registered with onset of MS after the year of the HA test and before follow-up, the expected number being 10.47 (P > > 0.05). Although Epstein-Barr virus is not suggested in itself to be the cause of MS, we propose that it is a co-factor in the pathogenesis of this disease.
Crystallization of butyrate kinase 2 fromThermotoga maritimamediated by vapor diffusion of acetic acid (2003)

The sitting-drop method of crystallization uses the evaporation of water to increase the concentration of the protein and precipitant in the drop. The presence of other volatile components, such as acetic acid, can have a marked impact on crystallization. A member of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of proteins, isobutyrate kinase (Buk2) from Thermotoga maritima, was expressed in Escherichia coli with six histidine residues added to the C-terminus. The purified protein was crystallized in a sitting drop with a well solution consisting of 1.7-3.0 M sodium formate, with the pH of the well solution alone adjusted to 4.5 with acetic acid. Diffraction data collected at 100 K show that the crystals diffract to 3.1 A and belong to space group I422, with unit-cell parameters a = b = 198.12, c = 58.93 A. Both the crystal form and the results of dynamic light-scattering studies suggest that Buk2 is an octomer, the first to be identified in the ASKHA superfamily.
Production of 2,4-diacetylphloroglucinol by the biocontrol agent Pseudomonas fluorescens Pf-5 (1994)

2,4-Diacetylphloroglucinol was detected in and isolated from culture extracts of the biological control bacterium Pseudomonas fluorescens Pf-5. Its structure was identified using a combination of chromatographic techniques and NMR spectroscopic methods. Carbon source influenced 2,4-diacetylphloroglucinol production by Pf-5 in culture media. 2,4-Diacetylphloroglucinol inhibited growth of the plant pathogenic fungi Pythium ultimum and Rhizoctonia solani, and the plant pathogenic bacterium Erwinia carotovora subsp. atroceptica, which cause diseases that are suppressed by strain Pf-5. The results of this study provide further evidence for the prevalence of 2,4-diacetylphloroglucinol production among strains of Pseudomonas fluorescens that suppress plant diseases.Key words: 2,4-diacetylphloroglucinol, Pseudomonas fluorescens, biocontrol.
On Secret Reconstruction in Secret Sharing Schemes (2008)

A secret sharing scheme typically requires secure communications in each of two distribution phases: (1) a dealer distributes shares to participants (share distribution phase); and later (2) the participants in some authorised subset send their share information to a combiner (secret reconstruction phase). While problems on storage required for participants, for example, the size of shares, have been well studied, problems regarding the communication complexity of the two distribution phases seem to have been mostly neglected in the literature so far. In this correspondence, we deal with several communication related problems in the secret reconstruction phase. Firstly, we show that there is a tradeoff between the communication costs and the number of participants involved in the secret reconstruction. We introduce the communication rate as the ratio of the secret size and the total number of communication bits transmitted from the participants to the combiner in the secret reconstruction phase. We derive a lower bound on the communication rate and give constructions that meet the bound. Secondly, we show that the point-to-point secure communication channels for participants to send share information to the combiner can be replaced with partial broadcast channels. We formulate partial broadcast channels as set systems and show that they are equivalent to the well-known combinatorial objects of cover-free family. Surprisingly, we find that the number of partial broadcast channels can be significantly reduced from the number of point-to-point secure channels. Precisely, in its optimal form, the number of channels can be reduced from n to O(log n), where is the number of participants in a secret sharing scheme. We also study the communication rates of partial broadcast channels for the secret reconstruction.
Cytotoxic T-lymphocyte response to autologous human squamous cell cancer of the lung: epitope reconstitution with peptides extracted from HLA-Aw68. (1994)

Cytotoxic T-lymphocytes (CTLs) specific for autologous human squamous cell cancer of the lung were generated by stimulation of peripheral blood lymphocytes with autologous tumor cells in vitro. The CTL line was >97% CD3+, CD8+, CD16- and produced tumor necrosis factor-alpha, gamma-interferon, and granulocyte-macrophage colony-stimulating factor after stimulation with autologous tumor. The CTLs lysed autologous tumor but failed to recognize autologous or histocompatibility leukocyte antigen-matched lymphoid cells, K562, or allogeneic tumor cells of several histological types. Antibody-blocking studies suggested that the CTLs recognized one or more antigens presented by the class I major histocompatibility complex molecule Aw68. To characterize these antigens further, histocompatibility leukocyte antigen Aw68 molecules were extracted from the squamous cell cancer of the lung tumor line by immunoaffinity chromatography, and the associated peptides were eluted in acid and separated by reversed-phase high-performance liquid chromatography. Reconstitution of the CTL epitope was evaluated by adding these peptides to autologous Epstein-Barr virus-transformed B-cells. Two peaks of reconstituting activity were observed, suggesting that these CTLs recognize at least two Aw68-associated peptides. This study confirms the existence of a CTL response against autologous human squamous cell cancer of the lung and suggests that this CTL response is directed against peptide epitopes presented by the class I major histocompatibility complex molecules. It is anticipated that this approach will permit identification of peptide epitopes for lung cancer-specific CTLs.
Administration of CD4+CD25highCD127− Regulatory T Cells Preserves β-Cell Function in Type 1 Diabetes in Children (2012)

This study shows that the administration of Tregs is safe and tolerable in children with recent-onset type 1 diabetes.
A prospective biomarker analysis of alvocidib followed by cytarabine and mitoxantrone in MCL-1-dependent relapsed/refractory acute myeloid leukemia (2021)

A prospective biomarker analysis of alvocidib followed by cytarabine
High-Affinity Ligand Probes of CD22 Overcome the Threshold Set by cis Ligands to Allow for Binding, Endocytosis, and Killing of B Cells (2006)

CD22 (Siglec-2) is a key regulator of B cell signaling whose function is modulated by interaction with extracellular glycan ligands mediated through its N-terminal Ig domain. Its preferred ligand is the sequence Sia alpha2-6Gal that is abundantly expressed on N-linked glycans of B cell glycoproteins, and by binding to CD22 in cis causes CD22 to appear "masked" from binding to synthetic sialoside probes. Yet, despite the presence of cis ligands, CD22 redistributes to sites of cell contact by binding to trans ligands on neighboring cells. In this study, we demonstrate the dynamic equilibrium that exists between CD22 and its cis and trans ligands, using a high-affinity multivalent sialoside probe that competes with cis ligands and binds to CD22 on native human and murine B cells. Consistent with the constitutive endocytosis reported for CD22, the probes are internalized once bound, demonstrating that CD22 is an endocytic receptor that can carry ligand-decorated "cargo" to intracellular compartments. Conjugation of the sialoside probes to the toxin saporin resulted in toxin uptake and toxin-mediated killing of B lymphoma cell lines, suggesting an alternative approach for targeting CD22 for treatment of B cell lymphomas.
Ribonuclease activity is associated with subviral particles isolated from interferon-treated vesicular stomatitis virus-infected cells (1988)

Previously we have shown that inhibition of replication of vesicular stomatitis virus in interferon-treated JLSV-11 cells is at least partly caused by impaired viral primary transcription. Here we report that subviral particles isolated from interferon-treated infected cells were deficient in mRNA synthesis in vitro compared with the particles isolated from untreated cells. This was due to the presence of an associated ribonuclease activity which hydrolyzed not only newly synthesized viral mRNAs but also exogenously added viral transcripts.