gebhart/patrepeval huggingface dataset:
PLA-based Runtime Dynamism in Support of Privacy-Enhanced Web Personalization (2006)

Software product line architectures (PLAs) have been widely recognized as a successful approach in industrial software development for improving productivity, software quality and time-to-market. In this paper, we focus on the usage of a PLA for a quite different purpose, namely, handling privacy constraints in Web personalization. To provide personalized services such as customized recommendations, a personalized Website collects users' personal data, which raises various privacy concerns. We aim at reconciling the benefits of web personalization with privacy constraints that come from users themselves as well as from privacy legislations and regulations that apply to a given user. We propose a dynamic, privacy-enabling personalization infrastructure and conceive it as a PLA. This infrastructure allows for dynamically selecting and instantiating personalization architectures that provide personalized services to each individual user and comply with the prevailing privacy constraints.
Old and new therapies in chronic lymphocytic leukemia: now is the time for a reassessment of therapeutic goals. (1998)

Chronic lymphocytic leukemia (CLL) is the most common adult leukemia for which therapeutic options remain unsatisfying as cure has remained elusive. Recent laboratory discoveries and promising results from completed phase III studies with fludarabine provide reason to reassess therapeutic goals in the treatment of patients with symptomatic CLL. Early enrollment of patients on protocols using combination therapies with fludarabine and new agents such as flavopiridol, IDEC-C2B8, Campath-1H, UCN-01, bryostatin, FR 901228, and melarsoprol will hopefully represent the next advance to improved overall survival and ultimately the cure of CLL.
Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii (2015)

Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s) and UDP glycosyltransferases (UGTs) in vivo. However, the relevant genes have not been characterized to date. In this study, we report successful identification of SgCbQ and UGT74AC1, which were previously predicted via RNA-sequencing (RNA-seq) and digital gene expression (DGE) profile analysis of the fruits of S. grosvenorii. SgCbQ was functionally characterized by expression in the lanosterol synthase-deficient yeast strain GIL77 and was found to accumulate cucurbitadienol as the sole product. UGT74AC1 was heterologously expressed in Escherichia coli as a His-tag protein and it showed specificity for mogrol by transfer of a glucose moiety to the C-3 hydroxyl to form mogroside IE by in vitro enzymatic activity assays. This study reports the identification of CbQ and glycosyltransferase from S. grosvenorii for the first time. The results also suggest that RNA-seq, combined with DGE profile analysis, is a promising approach for discovery of candidate genes involved in biosynthesis of triterpene saponins.
Sparse channel estimation for multicarrier underwater acoustic communication: From subspace methods to compressed sensing (2009)

In this paper, we present various channel estimators that exploit the channel sparsity in a multicarrier underwater acoustic system, including subspace algorithms from the array precessing literature, namely root-MUSIC and ESPRIT, and recent compressed sensing algorithms in form of Orthogonal Matching Pursuit (OMP) and Basis Pursuit (BP). Numerical simulation and experimental data of an OFDM block-by-block receiver are used to evaluate the proposed algorithms in comparison to the conventional least-squares (LS) channel estimator. We observe that subspace methods can tolerate small to moderate Doppler effects, and outperform the LS approach when the channel is indeed sparse. On the other hand, compressed sensing algorithms uniformly outperform the LS and subspace methods. Coupled with a channel equalizer mitigating intercarrier interference, the compressed sensing algorithms can handle channels with significant Doppler spread.
Lipophilic pro-oligonucleotides are rapidly and efficiently internalized in HeLa cells (1999)

Model t-Bu-SATE pro-dodecathymidines labeled with fluorescein and exhibiting various lipophilicities were evaluated for their uptake by cells in culture. Pro-oligonucleotides with appropriate lipophilicity were found to permeate across the HeLa cell membrane much more extensively than the control phosphorothioate oligo or than the hydrophilic pro-oligos. Fluorescence patterns of internalization were consistent with a diffusion mechanism resulting in the appearance of a uniform cytoplasmic distribution and nuclear accumulation, as confirmed by confocal microscopy.
Designs for a broad substrate specificity keto acid dehydrogenase (1990)

Variations have been made to the structure of the nicotinamide adenine dinucleotide (NAD) dependent L-lactate dehydrogenase from Bacillus stearothermophilus at regions of the enzyme that we believe determine specificity toward different alpha-hydroxy acids (RCHOHCOO-, R = CH3, C2H5, etc.). Two regions of LDH that border the active site (but are not involved in the catalytic reaction) were altered in order to accommodate substrates with hydrophobic side chains larger than that of the naturally preferred substrate, pyruvate (R = CH3). The mutations 102-105GlnLysPro----MetValSer and 236-237AlaAla----GlyGly were made to increase the tolerance for large hydrophobic substrate side chains. The triple and double mutants alone gave little improvement for branched-chain-substituted pyruvates. The five changes together produced a broader substrate specificity alpha-hydroxy acid dehydrogenase, with a 55-fold improved kcat for alpha-ketoisocaproate to a value about 1/14 that of the native enzyme for pyruvate. Rational protein engineering enabled coupled changes in enzyme structure to be obtained with greater probability of success than random mutagenesis.
Purification of the fibroblast growth factor activity from bovine brain. (1978)

The purification of the fibroblast growth factor (FGF) from bovine brain has led to the isolation of two peptides. FGF-1 with 128 amino acids and FGF-2 with 107 amino acids. The biological activity of these two peptides is acid- and heat-labile. As indicated by the amino acid composition of FGF-1 and -2, these two peptides are derived from a common precursor and bear no resemblance to pituitary FGF. The brain FGF peptides, like pituitary FGF, are mitogenic in vitro for the same wide variety of mesoderm-derived cells. Since their mitogenic activity is acid- and heat-labile, they are thereby distinguished from the platelet factor isolated from platelets and from the cationic peptide isolated from serum which has been shown to have the same molecular weight and isoelectric point as brain and pituitary FGF.
Transgenic Expression of Mouse Proinsulin II Prevents Diabetes in Nonobese Diabetic Mice (1997)

IDDM in humans and in nonobese diabetic (NOD) mice is a T-cell-dependent autoimmune disease in which the beta-cells of the pancreatic islets are destroyed. Several putative beta-cell autoantigens have been identified, but insulin and its precursor, proinsulin, are the only ones that are beta-cell specific. (Pro)insulin may be a key autoantigen in IDDM. To address the role of proinsulin in the development of IDDM, we generated NOD mice transgenic for the mouse proinsulin II gene driven off a major histocompatibility complex (MHC) class II promoter to direct expression of the transgene to MHC class II bearing cells, including those in the thymus, with the aim of deleting proinsulin-reactive T-cells. The mononuclear cell infiltration of the islets (insulitis) is almost completely absent, and diabetes is prevented in these transgenic NOD mice. The mononuclear cell infiltration of the salivary glands (sialitis) and immune responses to ovalbumin (OVA) are not altered, indicating that the protective effect of the transgene is specific for islet pathology and not due to general immunosuppression. We conclude that autoimmunity to proinsulin plays a pivotal role in the development of IDDM.
Expandable intrahepatic portacaval shunt stents in dogs with chronic portal hypertension (1986)

A canine experimental model was used to investigate the feasibility of using balloon-expandable portacaval shunts in humans with chronic portal hypertension. Intrahepatic portacaval shunts were created in nine dogs with stable portal hypertension previously induced by intraportal injections of polyvinyl alcohol (Ivalon). Embolic material was injected periodically through a subcutaneous port that allowed repeated access to the portal system. Shunts were placed 14 weeks after the last embolization. A shunt patency rate of 100% was observed up to 48 weeks. Low portacaval pressure gradient and high shunt flow accounted for the good results.
CH/π Attraction: The Origin of Enantioselectivity in Transfer Hydrogenation of Aromatic Carbonyl Compounds Catalyzed by Chiralη6-Arene-Ruthenium(II) Complexes (2001)

The through-space CH/π attraction between the η<sup>6</sup> -arene ligand on Ru and the carbonyl aryl substituent (see transition states in picture) plays a key role in the enantioselective transfer hydrogenation of aromatic carbonyl compounds with 2-propanol or formic acid, catalyzed by chiral η<sup>6</sup> -arene-Ru<sup>II</sup> complexes.
A GaAs-HBT A/D Gray-code converter (2002)

A monolithic high speed bipolar GaAs-HBT Analog-to-Digital converter is presented. The architecture approach is based upon a parallel conversion method. It relies on a structure mixing conventional flash converter elements without a complex output logic transcoder. We reached a sampling rate larger than 1 G-sample/s, with a 3-bit resolution.
Therapeutic Efficacy of Magnesium in Acoustic Trauma in the Guinea Pig (2003)

Comparative functional and morphological tests were performed in two groups of impulse noise-exposed guinea pigs treated either with magnesium (Mg) or isotonic saline as a placebo to extend the knowledge on the therapeutic efficacy of Mg in acoustic trauma demonstrated recently. The permanent threshold shifts were significantly lower in the Mg than in the placebo group as measured by auditory brainstem response audiometry, distortion product otoacoustic emissions and compound action potentials (CAPs) 1 week after exposure. This also applies to the damage to hair cell stereocilia tested with scanning electron microscopy. There were frequency-related differences in the individual functional responses. The CAP threshold shifts reflected the morphological damage most obviously.
Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana (1988)

Ethylene influences a number of developmental processes and responses to stress in higher plants. The molecular basis for the action of ethylene was investigated in mutants of Arabidopsis thaliana that have altered responses to ethylene. One mutant line, which has a dominant mutation at a locus designated etr, lacks a number of responses to ethylene that are present in the wild-type plant. These include inhibition of cell elongation, promotion of seed germination, enhancement of peroxidase activity, acceleration of leaf senescence, and feedback suppression of ethylene synthesis by ethylene. These diverse responses, which occur in different tissues of Arabidopsis, appear to share some common element in their transduction pathways-for example, a single receptor for ethylene. Results of ethylene binding experiments in vivo indicate that this receptor may be affected by the etr mutation.
Reduction of Renal Stone Risk by Potassium-Magnesium Citrate During 5 Weeks of Bed Rest (2007)

Provision of alkali as potassium-magnesium citrate is an effective countermeasure for the increased risk of renal stone disease associated with immobilization. Despite an increase in urine calcium concentration, the relative saturation of calcium oxalate decreased due to citrate chelation of calcium and the concentration of undissociated uric acid decreased due to the significant increase in urine pH.
Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. (1995)

We have reported that members of the bcl-2 gene family are expressed and gonadotropin regulated in ovarian granulosa cells during follicular maturation and atresia. Because Bcl-2, a protein that prevents apoptosis in several cell types, is reported to function as an antioxidant or free radical scavenger, the present studies were designed to investigate if oxidative stress plays a role in granulosa cell apoptosis during follicular atresia in the immature rat ovary. In the first series of experiments, the role of oxidative stress in the induction of granulosa cell apoptosis was directly tested using a defined in vitro follicle culture system. Healthy antral follicles obtained from equine CG (eCG)-primed immature (27 day old) rats were incubated in serum-free medium for 24 h in the absence or presence of FSH (100 ng/ml; a control for inhibiting apoptosis), superoxide dismutase (SOD; 10-1000 U/ml), ascorbic acid (0.01-1 mM; a free radical scavenger), N-acetyl-L-cysteine (25-100 mM; a free radical scavenger and stimulator of endogenous glutathione peroxidase activity), or catalase (10-1000 U/ml). Granulosa cells within follicles incubated in medium alone exhibited extensive apoptosis after 24 h of incubation, and this onset of apoptosis was blocked by treatment with FSH (29 +/- 4% of controls; P < 0.001, n = 3). Moreover, apoptosis in follicles was also inhibited by treatment with SOD (44 +/- 4% of controls at 1000 U/ml; P < 0.01, n = 3), ascorbic acid (55 +/- 9% of controls at 1 mM; P < 0.05, n = 3), N-acetyl-L-cysteine (24 +/- 7% of controls at 100 mM; P < 0.001, n = 3), or catalase (35 +/- 6% of controls at 1000 U/ml; P < 0.001, n = 3). In the second series of experiments, complementary DNAs corresponding to secreted (SEC-SOD), copper/zinc-containing (Cu/Zn-SOD), and manganese-containing (Mn-SOD) forms of rat SOD, rat seleno-cysteine glutathione peroxidase (GSHPx), and rat catalase were isolated and used to synthesize antisense RNA probes for Northern and slot blot analysis of changes in SOD, GSHPx, and catalase gene expression during follicular maturation. In vivo priming of 25-day-old female rats for 2 days with 10 IU eCG, which promoted antral follicular growth and survival, increased levels of messenger RNA encoding SEC-SOD (216 +/- 9% of saline-treated controls, P < 0.05, n = 3) and Mn-SOD (222 +/- 14% of saline-treated controls, P < 0.05, n = 3) vs. saline-treated controls.(ABSTRACT TRUNCATED AT 400 WORDS)
InJection adaptable Fine Ionization Source (‘JaFIS’) for continuous flow nano‐electrospray (1998)

Nanoelectrospray (‘nanoES’) tandem mass spectrometry of complex peptide mixtures has become a certified, mostly reliable technique for the identification of proteins. The typical low flow rates of nanoES, the extended analysis times for small samples, high ion transmission and its overall ease-of-use provide important practical advantages for polypeptide covalent microanalysis. We have constructed a modified nanoES ion source that is highly durable and user-friendly, and potentially allows for full auto-sampling operation. The inJection adaptable Fine Ionization Source (‘JaFIS’) can be operated at flow rates of 10–100 nL per minute and with sensitivities in the 25 femtomoles peptide per microliter range. The ion spray needles usually last for days, allowing for standards and multiple samples to be analyzed consecutively under similar conditions. In this configuration, quality controlled needles can also be saved and reused, providing for more consistent and reproducible day-to-day operating conditions. JaFIS-ES also permits sample recovery should any failure occur during analysis. © 1998 John Wiley & Sons, Ltd.
Algebraic Projective Geometry. (1953)

PART 1: THE ORIGINS AND DEVELOPMENT OF GEOMETRICAL KNOWLEDGE PART 2: ABSTRACT PROJECTIVE GEOMETRY
Purification, crystallization and preliminary X-ray analysis of the disintegrin contortrostatin fromAgkistrodon contortrix contortrixsnake venom (2002)

Disintegrins are cysteine-rich RGD-containing peptides that block tumor-cell implantation and angiogenesis. Contortrostatin, a homodimeric disintegrin (64 residues in each chain) from southern copperhead snake venom, has been purified to homogeneity and crystallized. Initial attempts at crystallization led to a form grown from polyethylene glycol (PEG), which crystallizes in the orthorhombic space group C222(1), with unit-cell parameters a = 57.39, b = 139.55, c = 78.98 A, and diffracts to a resolution limit of 3.2 A. Very recently, a new crystalline form of the title protein has been obtained grown from ammonium sulfate [(NH(4))(2)SO(4)] as a precipitant having a space group of P2(1)2(1)2(1), with unit-cell parameters a = 37.52, b = 59.93, c = 121.37 A. These improved crystals diffract to a resolution limit of 1.7 A.
An expressed neo(r) cassette provides required functions of the 1gamma2b exon for class switching (1998)

Germline CH transcripts initiate from a promoter upstream of a non-coding I exon, proceed through the switch (S) region and terminate downstream of the associated CH exons. To elucidate the role of germline transcription in Ig heavy chain class switch recombination (CSR), we used gene targeting in embryonic stem (ES) cells to replace most of the Igamma2b exon from immediately 3' of the majority of transcription initiation sites to beyond its donor splice site with a PGK-neo(r)gene inserted in the same transcriptional orientation as the endogenous unit. The mutation was introduced into both alleles of ES cell lines (referred to as gamma2-b(N/N)) and the neo(r) gene was deleted (referred to as Igamma2b-/-) by the loxP/Cre method. These mutations were assayed for effects on CSR in B cells derived via RAG-2-deficient blastocyst complementation. Igamma2b-/- B cells lacked ability to switch to IgG2b both in vivo and in vitro, and, correspondingly, showed no germline transcription through the Igamma2b exon, Sgamma2b or the Cgamma2b region. In contrast, Igamma2b(N/N) B cells switched at normal levels to IgG2b and showed substantial transcription through the Sgamma2b and Cgamma2b regions. Taken together, these results show that the Igamma2b sequences, per se, are not necessary for mediating CSR since a transcribed PGK-neo(r) gene can replace its function. However, the deleted portion of the Igamma2b exon and splice donor site apparently contain sequences necessary for efficient germline gene transcription and thus for CSR to IgG2b.
Expression of Functional CD32 Molecules on Human NK Cells Is Determined by an Allelic Polymorphism of the FcγRIIC Gene (1998)

Human natural killer (NK) cells were thought to express only FcgammaRIIIA (CD16), but recent reports have indicated that NK cells also express a second type of FcgammaR, ie, FcgammaRII (CD32). We have isolated, cloned, and sequenced full-length cDNAs of FcgammaRII from NK cells derived from several normal individuals that may represent four different products of the FcgammaRIIC gene. One transcript (IIc1) is identical with the already described FcgammaRIIc form. The other three (IIc2-IIc4) appear to represent unique, alternatively spliced products of the same gene, and include a possible soluble form. Analyses of the full-length clones have revealed an allelic polymorphism in the first extracellular exon, resulting in either a functional open reading frame isoform or a null allele. Stable transfection experiments enabled us to determine a unique binding pattern of anti-CD32 monoclonal antibodies to FcgammaRIIc. Further analyses of NK-cell preparations revealed heterogeneity in CD32 expression, ranging from donors lacking CD32 expression to donors expressing high levels of CD32 that were capable of triggering cytotoxicity. Differences in expression were correlated with the presence or absence of null alleles. These data show that certain individuals express high levels of functional FcgammaRIIc isoforms on their NK cells.
Development and performance of an all-DNA-based electro-optic waveguide modulator (2006)

Marine-based deoxyribonucleic acid (DNA), purified from waste products of the Japanese fishing industry, has recently become a new material of interest in photonics applications. The water soluble DNA is precipitated with a surfactant complex, hexadecyltrimethyl-ammonium chloride (CTMA), to form a water insoluble complex, DNA-CTMA, for application as a nonlinear optical material. In order to fabricate an all-DNA-based waveguide, it was necessary to crosslink the DNA-CTMA films. Crosslinking makes the films resistant to their initial solvent; this permits spin-coating of successive DNA-CTMA layers without solvent damage. A chromophore dye is added to the core layer to allow for an electro-optic coefficient to be induced through contact poling. Through contact poling, an electro-optic (EO) coefficient comparable to that in other polymers was demonstrated in crosslinked DNA-CTMA films with the chromophore dye Disperse Red 1. This EO effect allowed for the creation of the first all-DNA-based EO waveguide modulator. The performance of the modulator is described.
Synthesis and Nuclear Magnetic Resonance Structure Determination of an .alpha.-Helical, Bicyclic, Lactam-Bridged Hexapeptide (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Nuclear Magnetic Resonance Structure Determination of an .alpha.-Helical, Bicyclic, Lactam-Bridged HexapeptideClay Bracken, Jozsef Gulyas, John W. Taylor, and Jean BaumCite this: J. Am. Chem. Soc. 1994, 116, 14, 6431–6432Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://pubs.acs.org/doi/10.1021/ja00093a052https://doi.org/10.1021/ja00093a052research-articleACS PublicationsRequest reuse permissionsArticle Views468Altmetric-Citations94LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts