gebhart/patrepeval huggingface dataset:
Effect of lipopolysaccharide mutations on the pathogenesis of experimental Salmonella gastroenteritis (1983)

Lipopolysaccharide mutants of Salmonella typhimurium provoked diminished amounts of fluid in rabbit ileal loops as compared with the response to the wild type. The responses elicited by these mutants ranged from 0 to 60% of that caused by the parent strain. Two completely rough mutants and one leaky rough mutant were chosen for further study. Purified lipopolysaccharide from the parent and the mutant strains failed to stimulate fluid exsorption in ileal loop experiments. Histological studies revealed that the three lipopolysaccharide mutants were less invasive than wild type and were less able to generate an inflammatory reaction in the rabbit ileum. A Salmonella enterotoxin was present in culture filtrates from one rough mutant and the wild type; however, the rough mutant appeared to produce less toxin. Enterotoxic activity was absent in culture filtrates from the two other rough mutants. These results suggest that reductions in both invasiveness and the ability to produce Salmonella enterotoxin decreased the ability of these mutants to provoke fluid exsorption. Also, the results indicate that lipopolysaccharide mutations can have a profound effect on the enteropathogenic properties of S. typhimurium.
A 45nm Logic Technology with High-k+Metal Gate Transistors, Strained Silicon, 9 Cu Interconnect Layers, 193nm Dry Patterning, and 100% Pb-free Packaging (2007)

A 45 nm logic technology is described that for the first time incorporates high-k + metal gate transistors in a high volume manufacturing process. The transistors feature 1.0 nm EOT high-k gate dielectric, dual band edge workfunction metal gates and third generation strained silicon, resulting in the highest drive currents yet reported for NMOS and PMOS. The technology also features trench contact based local routing, 9 layers of copper interconnect with low-k ILD, low cost 193 nm dry patterning, and 100% Pb-free packaging. Process yield, performance and reliability are demonstrated on 153 Mb SRAM arrays with SRAM cell size of 0.346 mum <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , and on multiple microprocessors.
An Analysis of Different Area from Shadow Mask Using Morphological Operations (1993)

When we recognize the shape of an object from an image to store in a computer, we use the pattern matching. The typical pattern matching technique is detection of a similarity of the shape of images. But, these techniques can not analyze a difference in the shape. This paper show the features of the shape by using numerical values, and take notice of morphology. The features of the shape by morphology is technique to describe pattern spectrum. This technique is more conscious to the shape of the image than other pattern matching technique, when extracting a different area from the texture image. At first, we express the features of the shape by pattern spectrum to use binary morphology. This pattern spectrum value has disposition that depends on the resolution, the shape of structuring element, and turning of an image. Next, we extend a gray scale image, and we consider its pattern spectrum to discriminate different areas of a shadow mask image. Analyzing different areas from a shadow mask we can detect a defective part of a repeating pattern on a grey scal image.
Production of Proinflammatory Cytokines by Phorbol Myristate Acetate-Treated THP-1 Cells and Monocyte-Derived Macrophages After Phagocytosis of Apoptotic CTLL-2 Cells (1998)

Because it is generally believed that apoptosis is not associated with inflammation, we hypothesized that the interaction of phagocytes with apoptotic cells provides a negative or null signal for inflammation. However, we recently found that the interaction led to the production of proinflammatory cytokines but not antiinflammatory cytokines, although the apoptotic cell membranes appeared to be intact. In this study, we examined in detail the relationship among the kinetics of apoptosis, phagocytosis and production of cytokines by macrophages. Among the time points examined, murine CTLL-2 cells became apoptotic in terms of cell size and exposure of phosphatidylserine after 12 h of culture in the absence of IL-2, and at the same time they began to be phagocytosed and lead to proinflammatory cytokine production by PMA-treated THP-1 cells (human macrophages). The phagocytosis of apoptotic cells by macrophages was also confirmed by confocal laser microscopy. The coculturing of human macrophages with murine apoptotic cells led to the production of human proinflammatory cytokines, notably IL-8, at both the mRNA level and the protein level. The coculturing of monocyte-derived macrophages with the apoptotic cells also led to the production of IL-8 protein. Both the phagocytosis and production of the cytokines were suppressed by either phospho-L-serine or RGDS (Arg-Gly-Asp-Ser), but not by RGES (Arg-Gly-Glu-Ser). Thus, the production of proinflammatory cytokines and phagocytosis of apoptotic CTLL-2 cells appear to be closely interrelated.
Deaths Caused by Bedrails (1997)

Deaths from bedrails are underrecognized and preventable clinical events that can occur in any medical setting. Preventing these events will require a unified redesign of the relationships between rails, mattresses, and beds, which are now often assembled and used as separate products. Clinicians can prevent many of these deaths by using bedrails much more judiciously, confirming the proper relationships between beds, rails and mattresses, and using alarms.
Strontium Ranelate Increases Cartilage Matrix Formation (2001)

Based on previous studies showing that strontium ranelate (S12911) modulates bone loss in osteoporosis, it could be hypothesized that this drug also is effective on cartilage degradation in osteoarthritis (OA). This was investigated in vitro on normal and OA human chondrocytes treated or not treated with interleukin-1beta (IL-1beta). This model mimics, in vitro, the imbalance between chondroformation and chondroresorption processes observed in vivo in OA cartilage. Chondrocytes were isolated from cartilage by enzymatic digestion and cultured for 24-72 h with 10(-4)-10(-3) M strontium ranelate, 10(-3) M calcium ranelate, or 2 x 10(-3) M SrCl2 with or without IL-1beta or insulin-like growth factor I (IGF-I). Stromelysin activity and stromelysin quantitation were assayed by spectrofluorometry and enzyme amplified sensitivity immunoassay (EASIA), respectively. Proteoglycans (PG) were quantified using a radioimmunoassay. Newly synthesized glycosaminoglycans (GAGs) were quantified by labeled sulfate (Na2(35)SO4) incorporation. This method allowed the PG size after exclusion chromatography to be determined. Strontium ranelate, calcium ranelate, and SrCl2 did not modify stromelysin synthesis even in the presence of IL-1beta. Calcium ranelate induced stromelysin activation whereas strontium compounds were ineffective. Strontium ranelate and SrCl2 both strongly stimulated PG production suggesting an ionic effect of strontium independent of the organic moiety. Moreover, 10(-3) M strontium ranelate increased the stimulatory effect of IGF-I (10(-9) M) on PG synthesis but did not reverse the inhibitory effect of IL-1beta. Strontium ranelate strongly stimulates human cartilage matrix formation in vitro by a direct ionic effect without stimulating the chondroresorption processes. This finding provides a preclinical basis for in vivo testing of strontium ranelate in OA.
Lung Cancer, Cardiopulmonary Mortality, and Long-term Exposure to Fine Particulate Air Pollution (2002)

Long-term exposure to combustion-related fine particulate air pollution is an important environmental risk factor for cardiopulmonary and lung cancer mortality.
Options for multiple sclerosis therapy (1994)

TWO NEW drugs, apparently acting in different ways, may soon provide clinical options in treating multiple sclerosis. Only a year ago there were no effective agents on the market to delay the course of autoimmune nerve demyelination. The Food and Drug Administration late in 1993 approved interferon beta-1b (Betaseron, Chiron Corp, Emeryville, Calif), based on data showing a significantly reduced relapse rate. Now two other agents appear not only to reduce the remission rate but also to slow disease progression and delay the onset of physical disability, according to reports at the annual meeting of the American Neurological Association, held in San Francisco, Calif. It is "very significant" that the drugs showed an effect on the rate of progression, says Abe Eastwood, PhD, director of research grant programs at the National Multiple Sclerosis Society, New York, NY. "There has never been a drug that's had" such an effect. While direct
Semantic Data Caching and Replacement (1996)

We propose a semantic model for client-side caching and replace-ment in a client-server database system and compare this approach to page caching and tuple caching strategies. Our caching model is based on, and derives its advantages from, three key ideas. First, the client maintains a semantic description of the data in its cache,which allows for a compact specification, as a remainder query, of the tuples needed to answer a query that are not available in the cache. Second, usage information for replacement policies is maintained in an adaptive fashion for semantic regions, which are associated with collections of tuples. This avoids the high overheads of tuple caching and, unlike page caching, is insensi-tive to bad clustering. Third, maintaining a semantic description of cached data enables the use of sophisticated value functions that incorporate semantic notions of locality, not just LRU or MRU, for cache replacement. We validate these ideas with a detailed performance study that includes traditional workloads as well as a workload motivated by a mobile navigation application.
Erythropoietin structure-function relationships: high degree of sequence homology among mammals (1993)

To investigate structure-function relationships of erythropoietin (Epo), we have obtained cDNA sequences that encode the mature Epo protein of a variety of mammals. A first set of primers, corresponding to conserved nucleotide sequences between mouse and human DNAs, allowed us to amplify by polymerase chain reaction (PCR) intron 1/exon 2 fragments from genomic DNA of the hamster, cat, lion, dog, horse, sheep, dolphin, and pig. Sequencing of these fragments permitted the design of a second generation of species-specific primers. RNA was prepared from anemic kidneys and reverse-transcribed. Using our battery of species-specific 5' primers, we were able to successfully PCR-amplify Epo cDNA from Rhesus monkey, rat, sheep, dog, cat, and pig. Deduced amino acid sequences of mature Epo proteins from these animals, in combination with known sequences for human, Cynomolgus monkey, and mouse, showed a high degree of homology, which explains the biologic and immunological cross-reactivity that has been observed in a number of species. Human Epo is 91% identical to monkey Epo, 85% to cat and dog Epo, and 80% to 82% to pig, sheep, mouse, and rat Epos. There was full conservation of (1) the disulfide bridge linking the NH2 and COOH termini; (2) N-glycosylation sites; and (3) predicted amphipathic alpha-helices. In contrast, the short disulfide bridge (C29/C33 in humans) is not invariant. Cys33 was replaced by a Pro in rodents. Most of the amino acid replacements were conservative. The C-terminal part of the loop between the C and D helices showed the most variation, with several amino acid substitutions, deletions, and/or insertions. Calculations of maximum parsimony for intron 1/exon 2 sequences as well as coding sequences enabled the construction of cladograms that are in good agreement with known phylogenetic relationships.
Complete cDNA sequence of a human dioxin-inducible mRNA identifies a new gene subfamily of cytochrome P450 that maps to chromosome 2. (1994)

Previously, levels of a novel human mRNA, detected by a recombinant cDNA designated clone 1, were shown to be increased 50-fold in response to treatment of a keratinocyte cell line with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), in part as a function of increased rates of gene transcription (Sutter, T.R., Guzman, K., Dold, K.M., and Greenlee, W.F. (1991) Science 254, 415-418). Here we report the complete corresponding 5.1-kilobase cDNA sequence. A single open reading frame that predicts a protein of 543 amino acid residues was determined by computer-assisted analysis of the cDNA sequence. This predicted protein identifies a new gene subfamily of cytochrome P450, cytochrome P4501B1 (CYP1B1), that maps to human chromosome 2. Southern blot analysis of genomic DNA indicates that the human CYP1B subfamily is likely to contain only this single gene. Northern blot analysis of RNA isolated from primary cultures of normal human epidermal keratinocytes showed approximately 100-fold increased levels of the CYP1B1 mRNA after treatment with 10 nM TCDD for 24 h. Low levels of constitutive CYP1B1 mRNA were detected in 15 different human tissue samples. These results indicate that CYP1B1 is expressed in many normal human tissues and advance our understanding of the complexity of a gene family of cytochromes P450 whose expression is altered by TCDD.
Authenticating real time packet streams and multicasts (2003)

We propose a new stream authentication scheme that is suitable for live packet streams distributed over a lossy channel, such as an IP-multicast group. Packets are signed together in a block and the recipient can authenticate this block if the loss rate per block is lower than a certain threshold, which can be chosen based on the characteristic of the communication channel. This scheme provides both integrity and non repudiation of origin, and in a majority of situations, it performs with less overhead in bytes per packet than previously proposed practical live stream authentication schemes.
Using Peano Curves for Bilevel Display of Continuous-Tone Images (1982)

A new method for the black-and-white display of gray-scale images limits cumulative error in binary subdivisions of images and eliminates deficiencies of the ordered dither technique.
Delayed clearance of apoptotic lymphoma cells allows cross-presentation of intracellular antigens by mature dendritic cells (1999)

Single cells are deleted from the midst of living tissue during normal turnover and embryogenesis. This event is not associated with inflammation or autoimmunity. Little is known of the clearance of apoptotic cells during dangerous situations, accompanied by extensive cell death and tissue damage: when macrophages are overwhelmed by apoptotic cells, other phagocytes, including immature dendritic cells (DCs), may become involved. DCs efficiently present antigens derived from the processing of internalized apoptotic bodies to class I- and class II-restricted T cells. Antigen presentation results either in T cell activation or in their functional blockade. The outcome is influenced by pro-inflammatory maturative signals: efficient T cell cross-priming requires fully mature DCs. Here we discuss in vitro data suggesting that the number of apoptotic cells that die at a given time influences DC maturation and therefore their ability to uptake antigens from apoptotic cells and cross-activate T lymphocytes.
Reversion of FMR1 Methylation and Silencing by Editing the Triplet Repeats in Fragile X iPSC-Derived Neurons (2015)

Fragile X syndrome (FXS) is the most common form of inherited intellectual disability, resulting from a CGG repeat expansion in the fragile X mental retardation 1 (FMR1) gene. Here, we report a strategy for CGG repeat correction using CRISPR/Cas9 for targeted deletion in both embryonic stem cells and induced pluripotent stem cells derived from FXS patients. Following gene correction in FXS induced pluripotent stem cells, FMR1 expression was restored and sustained in neural precursor cells and mature neurons. Strikingly, after removal of the CGG repeats, the upstream CpG island of the FMR1 promoter showed extensive demethylation, an open chromatin state, and transcription initiation. These results suggest a silencing maintenance mechanism for the FMR1 promoter that is dependent on the existence of the CGG repeat expansion. Our strategy for deletion of trinucleotide repeats provides further insights into the molecular mechanisms of FXS and future therapies of trinucleotide repeat disorders.
La idea de nación en 1810 (2006)

Identificar el concepto de nacion que tenian los hombres que decidieron la emancipacion de la corona espanola implica un trabajo importante de interpretacion, pues la evolucion de dicha idea es un fenomeno bien conocido por los historiadores de los conceptos. En tal sentido, el presente analisis ayuda a comprender el contexto de una discusion nunca acabada, y como bien se senala en las palabras finales: “La Junta de Gobierno de 1810 debe ser una de las creaciones mas originales de la historia institucional chilena. No obstante, si bien su formacion marca uno de los quiebres mas profundos de nuestro pasado, no es revolucionaria en si”.
Discovering High-Affinity Ligands for Proteins: SAR by NMR (1996)

A nuclear magnetic resonance (NMR)-based method is described in which small organic molecules that bind to proximal subsites of a protein are identified, optimized, and linked together to produce high-affinity ligands. The approach is called "SAR by NMR" because structure-activity relationships (SAR) are obtained from NMR. With this technique, compounds with nanomolar affinities for the FK506 binding protein were rapidly discovered by tethering two ligands with micromolar affinities. The method reduces the amount of chemical synthesis and time required for the discovery of high-affinity ligands and appears particularly useful in target-directed drug research.
A parametric approach to vocal tract length normalization (2002)

Differences in vocal tract size among individual speakers contribute to the variability of speech waveforms. The first-order effect of a difference in vocal tract length is a scaling of the frequency axis; a female speaker, for example, exhibits formants roughly 20% higher than the formants of from a male speaker, with the differences most severe in open vocal tract configurations. We describe a parametric method of normalisation which counteracts the effect of varied vocal tract length. The method is shown to be effective across a wide range of recognition systems and paradigms, but is particularly helpful in the case of a small amount of training data.
Different active sites of mammalian DNA ligases I and II. (1994)

Bovine DNA ligases I and II were adenylylated in the presence of [alpha-32P]ATP and digested with limiting amounts of trypsin or V8 protease. The generation of radioactive peptides of decreasing size was monitored by polyacrylamide gel electrophoresis and autoradiography. Active site peptides obtained by complete proteolytic digestions with trypsin, V8, or Lys-C protease were also compared. The partial digestion products of DNA ligases I and II were entirely different, with no indication of extensive sequence homology. Furthermore, the sequence of the active site region of DNA ligase I is clearly different from that of DNA ligase II. Similar analysis of a third chromatographically distinct mammalian DNA ligase indicated that it is different from DNA ligase I but related to DNA ligase II.
Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer (2011)

A comprehensive understanding of the molecular vulnerabilities of every type of cancer will provide a powerful roadmap to guide therapeutic approaches. Efforts such as The Cancer Genome Atlas Project will identify genes with aberrant copy number, sequence, or expression in various cancer types, providing a survey of the genes that may have a causal role in cancer. A complementary approach is to perform systematic loss-of-function studies to identify essential genes in particular cancer cell types. We have begun a systematic effort, termed Project Achilles, aimed at identifying genetic vulnerabilities across large numbers of cancer cell lines. Here, we report the assessment of the essentiality of 11,194 genes in 102 human cancer cell lines. We show that the integration of these functional data with information derived from surveying cancer genomes pinpoints known and previously undescribed lineage-specific dependencies across a wide spectrum of cancers. In particular, we found 54 genes that are specifically essential for the proliferation and viability of ovarian cancer cells and also amplified in primary tumors or differentially overexpressed in ovarian cancer cell lines. One such gene, PAX8, is focally amplified in 16% of high-grade serous ovarian cancers and expressed at higher levels in ovarian tumors. Suppression of PAX8 selectively induces apoptotic cell death of ovarian cancer cells. These results identify PAX8 as an ovarian lineage-specific dependency. More generally, these observations demonstrate that the integration of genome-scale functional and structural studies provides an efficient path to identify dependencies of specific cancer types on particular genes and pathways.
Tissue Response to Biodegradable Injectable Microcapsules (1987)

Biodegradable microcapsules have been shown to be capable of delivering a sustained release of various medicinal agents. We have observed an initial minimal, sharply localized, acute inflammatory response to intramuscularly injected microcapsules produced from various polymers. Evaluation of later time points has shown a diminishing macrophage, foreign body giant cell, and connective tissue response along with actual microcapsule degradation. No fibrous capsule formation was seen. Complete resolution of the tissue reaction can be expected, with the time frame depending upon the polymer utilized. Published experimental results report similar minor tissue responses to a variety of injected biodegradable microcapsules.
Failure of a heat and moisture exchanger as a cause of disconnection during anaesthesia (1989)

Two cases of ventilator tubing mishaps, resulting from defective heat and moisture exchangers, are described. The report emphasises the need for preoperative inspection of the anaesthetic machine and associated equipment as well as the importance of a disconnect alarm device.