gebhart/patrepeval huggingface dataset:
The peptide way to macrocyclic bifunctional chelating agents: synthesis of 2-(p-nitrobenzyl)-1,4,7,10-tetraazacyclododecane-N,N',N",N'''-tetraacetic acid and study of its yttrium(III) complex (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe peptide way to macrocyclic bifunctional chelating agents: synthesis of 2-(p-nitrobenzyl)-1,4,7,10-tetraazacyclododecane-N,N',N",N'''-tetraacetic acid and study of its yttrium(III) complexMin K. Moi, Claude F. Meares, and Sally J. DeNardoCite this: J. Am. Chem. Soc. 1988, 110, 18, 6266–6267Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August 1988https://pubs.acs.org/doi/10.1021/ja00226a063https://doi.org/10.1021/ja00226a063research-articleACS PublicationsRequest reuse permissionsArticle Views1182Altmetric-Citations149LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Design and Application of a Biosensor for Monitoring Toxicity of Compounds to Eukaryotes (2000)

Here we describe an alternative approach to currently used cytotoxicity analyses through applying eukaryotic microbial biosensors. The yeast Saccharomyces cerevisiae was genetically modified to express firefly luciferase, generating a bioluminescent yeast strain. The presence of any toxic chemical that interfered with the cells' metabolism resulted in a quantitative decrease in bioluminescence. In this study, it was demonstrated that the luminescent yeast strain senses chemicals known to be toxic to eukaryotes in samples assessed as nontoxic by prokaryotic biosensors. As the cell wall and adaptive mechanisms of S. cerevisiae cells enhance stability and protect from extremes of pH, solvent exposure, and osmotic shock, these inherent properties were exploited to generate a biosensor that should detect a wide range of both organic and inorganic toxins under extreme conditions.
Transgenic maize: methods and protocols. (2009)

As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs
Gastroplasty for obesity: Long‐term weight loss improved by vagotomy (1993)

Sixty-nine (13M, 56F), severely obese patients (body mass index 47 kg/m2) have had vertical banded gastroplasty (GP) with 5-cm polypropylene mesh (n = 39) or fascia (n = 30) bands since 1981. Of these 69 patients, 30 also had truncal vagotomy (TVG) without drainage. Total office follow-up rate is 94%. During follow-up of 1 year or more (mean 60 months), 25 patients with vagotomy plus gastroplasty lost 33 +/- 3 kg, corresponding to 51% of excess weight, compared to 21 +/- 3 kg (34% excess weight) in the 34 patients having gastroplasty alone (p < 0.01). In patients followed > or = 5 years (mean 83 months) 10 patients with TVG lost 40 +/- 5 kg (61% of excess) compared to 17 +/- 4 kg (28% of excess) in 22 patients with GP alone (p < 0.001). Frequency and severity of complications were similar in both groups, but there were seven reoperations after GP and three after TVG (p < 0.05). Studies of gastric emptying of a solid meal in 14 of the patients with GP and 14 with TVG demonstrated greater weight loss in those with prolonged emptying and gastroesophageal pooling, though the emptying rates of patients with GP and those with TVG showed no statistically significant difference. Our earlier studies, which showed reduced liquid consumption after vagotomy, imply that this mechanism (rather than delayed emptying) explains why vagotomy potentiates weight loss after gastroplasty.
Generation of RAGE‐1 and MAGE‐9 peptide‐specific cytotoxic T‐Lymphocyte lines for transfer in patients with renal cell carcinoma (2005)

Renal cell carcinomas (RCCs) are supposed to be immunogenic, and several clinical trials of immunotherapy using tumor lysate-pulsed dendritic cells (DCs) have been performed. We report on the generation of RAGE-1 and MAGE-9 peptide-specific CTL lines. RAGE-1 and MAGE-9 are expressed in 56% and 38% of RCCs. Seven MAGE-9- and 13 RAGE-1-derived peptides were found to be immunogenic in the context of the HLA-A*0201 MHC. CTLs were generated by coculture with peptide-pulsed, activated B cells, which were easily generated in great quantities and displayed functional activity for a prolonged period of time. MAGE-9 and RAGE-1 peptide-specific CTL lines were strictly peptide-specific and displayed high cytotoxic activity not only against peptide-loaded T2 cells but also against HLA-A*0201-positive RCC lines, which naturally express MAGE-9, RAGE-1 or both. Thus, B cells are well suited as APCs for the generation of large numbers of tumor peptide-specific CTLs for adoptive transfer. MAGE-9 as well as RAGE-1 may well provide suitable targets for immunotherapy of RCC.
Object model creation from multiple range images: acquisition, calibration, model building and verification (2002)

This paper demonstrates the accuracy of a prototype Laser Range Camera (LRC) developed at the National Research Council of Canada for the creation of models of real objects. A laser survey performed in collaboration with the Canadian Space Agency and NASA is used as a test case. The object selected for this particular test case is the Orbiter Docking System (ODS) located at the Kennedy Space Center, Florida. During the laser survey, 128 range (and registered intensity) images were acquired all around the ODS. These images were then processed in our laboratory. A full model of the top portion of the ODS was created along with an almost complete model of the ODS. The ODS has a diameter of 1.6 m and a height of 3.9 m. Targets mounted on the top portion of the ODS were used to assess the accuracy of the calibration and of the image registration process. These targets were measured with a network of theodolites a day prior to the laser survey and used as a reference. With the current calibration and range image registration techniques, an accuracy better than 0.25 mm in X and Y, and, 0.80 mm in Z was achieved. These results compare favorably with the single point accuracy obtained after calibration, i.e., about 0.25 mm in X and Y, and, 0.50 mm in Z. These figures and others should testify on the usefulness of a LRC for accurate model building.
Intramolecular Reaction in Polycondensations. I. The Theory of Linear Systems (1950)

For three types of linear polycondensing systems, equilibrium molecular size distributions, including rings as allowable species, are derived. Average molecular weights and amounts of ring and chain fractions are calculated therefrom. The fractions of rings are shown to increase with dilution, and with molecular weight. It is shown that beyond a critical dilution it is sometimes possible to obtain 100 percent yield of rings by driving the condensation to completion. Detailed calculations are made for two important cases corresponding to condensations of the decamethylene glycol-adipic acid type: (1) for equimolar amounts of the two monomers, and (2) unequal amounts, with one monomer type completely reacted.
The GORE-TEX expanded polytetrafluoroethylene prosthetic ligament. An in vitro and in vivo evaluation. (1985)

For assessment of the accelerated in vitro and in vivo performance of the GORE-TEX Prosthetic Ligament, mechanical testing included cyclic creep and bending fatigue. Elongation of less than 3% after 20,000,000 cycles under load conditions was used to simulate the functioning human anterior cruciate ligament (ACL). Device tensile strength was 4830 N +/- 280 N. The device was tested in 17 sheep as an ACL replacement. No immobilization was used postoperation. Implant time ranged from 0 to 369 days. Joint stability was maintained in all animals. Fixation strength resulting from osseous ingrowth reached an average of 1380 N at a mean implant duration of 218 days. Clinical trials are underway at 15 centers in the United States. The indication for polytetrafluoroethylene (PTFE) reconstruction is chronic insufficiency of the ACL in knees worthy of salvage. The following evaluation of 130 patients at 15 months or less shows improved stability in 129. Two grafts have been removed--one secondary to infection and the other as a result of improper placement.
SlipChip for Immunoassays in Nanoliter Volumes (2010)

This article describes a SlipChip-based approach to perform bead-based heterogeneous immunoassays with multiple nanoliter-volume samples. As a potential device to analyze the output of the chemistrode, the performance of this platform was tested using low concentrations of biomolecules. Two strategies to perform the immunoassay in the SlipChip were tested: (1) a unidirectional slipping method to combine the well containing a sample with a series of wells preloaded with reagents and (2) a back-and-forth slipping method to introduce a series of reagents to a well containing the sample by reloading and slipping the well containing the reagent. The SlipChips were fabricated with hydrophilic surfaces on the interior of the wells and with hydrophobic surfaces on the face of the SlipChip to enhance filling, transferring, and maintaining aqueous solutions in shallow wells. Nanopatterning was used to increase the hydrophobic nature of the SlipChip surface. Magnetic beads containing the capture antibody were efficiently transferred between wells and washed by serial dilution. An insulin immunoenzymatic assay showed a detection of limit of approximately 13 pM. A total of 48 droplets of nanoliter volume were analyzed in parallel, including an on-chip calibration. The design of the SlipChip is flexible to accommodate other types of immunoassays, both heterogeneous and homogeneous. This work establishes the possibility of using SlipChip-based immunoassays in small volumes for a range of possible applications, including analysis of plugs from a chemistrode, detection of molecules from single cells, and diagnostic monitoring.
Layer splitting process in hydrogen-implanted Si, Ge, SiC, and diamond substrates (1997)

Si, Ge, SiC, and diamond samples were implanted with H2+ at 120–160 keV with 5.0×1016 ions/cm2 (corresponding to 1.0×1017 H+ ions/cm2) and annealed at various temperatures to introduce hydrogen filled microcracks. An effective activation energy was determined for the formation of optically detectable surface blisters from the time required to form such blisters at various temperatures. The measured effective activation energies are close to the respective bond energies in all four materials. The time required to completely split hydrogen implanted layers from bonded silicon substrates and to transfer them onto oxidized silicon wafers is a factor of about 10 longer. Both processes, blister formation and layer splitting, show the same activation energy.
Boosting the margin: A new explanation for the effectiveness of voting methods (1997)

. One of the surprising recurring phenomena observed in experiments with boosting is that the test error of the generated classifier usually does not increase as its size becomes very large, and often is observed to decrease even after the training error reaches zero. In this paper, we show that this phenomenon is related to the distribution of margins of the training examples with respect to the generated voting classification rule, where the margin of an example is simply the difference between the number of correct votes and the maximumnumber of votes received by any incorrect label. We show that techniques used in the analysis of Vapnik&amp;apos;s support vector classifiers and of neural networks with small weights can be applied to voting methods to relate the margin distribution to the test error. We also show theoretically and experimentally that boosting is especially effective at increasing the margins of the training examples. Finally, we compare our explanation to those based on the ...
Surface-Emitting AlGaAs/GaAs DH LED with Buried-Window Cylindrical Lens (1988)

Surface-emitting AlGaAs/GaAs double heterostructure (DH) LED's with buried-window cylindrical lenses have been fabricated. The selective meltback and regrowth were used for the lens and DH junctions. The far-field pattern has the full width at half maximum (FWHM) of 90°. A small protrusion with the full width of 20° was observed in the far-field pattern. The maximum power of 20 mW was obtained from the 1 µm×60 µm×300 µm active layer.
The respiratory chain of Helicobacter pylori: identification of cytochromes and the effects of oxygen on cytochrome and menaquinone levels (1996)

The quinone and cytochrome components of the respiratory chain of the microaerophilic bacterium Helicobacter pylori have been investigated. The major isoprenoid quinone was menaquinone-6, with traces of menaquinone-4; no methyl-substituted or unusual menaquinone species were found. Cell yield was highest after growth at 10% (v/v) oxygen and menaquinone levels (per dry cell mass) were maximal at 5–10% (v/v) oxygen. Helicobacter pylori cells and membranes contained b-and c-type cytochromes, but not terminal oxidases of the a-or d-types, as judged by reduced minus oxidised difference spectra. Spectra consistent with the presence of a CO-binding terminal oxidase of the cytochrome b-or o-type were obtained. The soluble fraction from disrupted cells also contained cytochrome c. There were no significant qualitative differences in the cytochrome complements of cells grown at oxygen concentrations in the range 2–15% (v/v) but putative oxidases were highest in cells grown at 5–10% (v/v) oxygen.
The isolated iron–molybdenum cofactor of nitrogenase binds carbon monoxide upon electrochemically accessing reduced states (1999)

The first spectroscopic evidence for the binding of a small gaseous molecule to the isolated iron molybdenum cofactor of nitrogenase (FeMoco) is presented: FTIR spectroelectrochemistry in a thin-layer cell shows that reduced FeMoco binds carbon monoxide and gives rise to ν(CO) stretches that are close to those observed during turnover of the enzyme.
Molecular characterization of the 7q deletion in myeloid disorders (1996)

Deletion of the long arm of chromosome 7 is a common karyotypic finding in myeloid disorders and in particular is found in association with secondary leukaemias. We have used restriction fragment length polymorphisms and gene dosage experiments to assess the loss or retention of sequences localized to chromosome 7q in five patients with clonal myeloid disorders and a 7q deletion. The deletion was interstitial in all cases with retention of the anonymous marker pS194 located at 7q36-qter. Three out of five cases also retained the more proximal gene T-cell receptor beta (TCR beta) located at 7q35. The proximal breakpoints of all five cases were localized to 7q22 by cytogenetic analysis. In two cases the proximal breakpoint lay between the genes for elastin (ELN) and collagen type 1 alpha (COL1A2) and in three cases distal to this region between the genes for erythropoietin (EPO) and acetylcholinesterase (ACHE). The genes of ACHE, plasminogen activator inhibitor 1 (PLANH1), CCAAT displacement protein (CUTL1) and Met proto-oncogene (MET) were deleted in all cases. Molecular analysis of the 7q deletion in myeloid leukaemias demonstrates heterogeneity of the breakpoints, supporting a recessive mechanism of tumourigenesis.
Modulation of polyglutamine-induced cell death by genes identified by expression profiling (2002)

The majority of triplet-repeat diseases are caused by mutated genes with an extended polyglutamine tract, exemplified by Huntington's disease (HD). In order to model HD pathogenesis in a controlled system, we developed stable PC12 cell lines that express exon 1 fragments of the huntingtin gene with 23 or 74 polyglutamines driven by an inducible doxycycline (dox)-sensitive promoter (HD-23Q or HD-74Q). We aimed to identify early perturbations induced by the mutation by studying expression levels of 1824 genes at 0, 5, 10 and 18 hours after induction, using adaptor-tagged competitive PCR (ATAC-PCR). At these time points, the cells show no appreciable death or mitochondrial impairment and very low inclusion levels. A total of 126 genes, including 69 known genes, exhibited statistically significant alterations in the HD-74Q cell lines but no changes in the HD-23Q lines. We tested 11 of these genes for their abilities to modulate polyglutamine-induced cell death in transiently transfected cell models. Five genes [glucose transporter 1 (Glut1), phosphofructokinase muscle isozyme (Pfkm), prostate glutathione-S -transferase 2 (Gstm2), RNA-binding motif protein 3 (Rbm3) and KRAB-A interacting protein 1 (Krip-1)] significantly suppressed cell death in both neuronal precursor and non-neuronal cell lines, suggesting that these transcriptional changes were relevant to polyglutamine pathology. The efficient recovery of functionally relevant genes supports the utility of gene expression profiling for discovering pathways related to pathogenesis, and the importance of analyzing molecular events in the early stages of disease.
Temperature Changes in Chicken Embryos Exposed to a Continuous-Wave 1.25 GHz Radiofrequency Electromagnetic Field (2003)

A total of 550 fertile chicken eggs (White Leghorn) were exposed to a radiofrequency (RF) electromagnetic field of 1.25 GHz (continuous wave) at six different power flux densities in the range of 9.0-0.75 mW/cm(2). The eggs were exposed either continuously throughout the whole 21 days of incubation (long-term exposure) or in a short-term exposure (1-2 h/day). The temperatures of the embryonic tissue and the amniotic fluid, respectively, were measured with inserted temperature probes. This study was designed to investigate the relationship between exposure and temperature changes in exposed tissues, without considering biological and medical effects. This knowledge is of general interest for studies of nonthermic teratological or embryo-lethal effects of exposure to electromagnetic fields (EMFs). Throughout the entire 21 days of embryonic development, the mean temperature increases in the eggs during the exposure were found to be up to 0.25 degrees C for a power flux density of 1.25 mW/cm(2) and increased to 2.3 degrees C for 9.0 mW/cm(2). The corresponding maximum whole-body SARs for the embryos over the 21 days of embryonic development were 1.45 and 10.44 W/kg, respectively. At 0.75 mW/cm(2) (0.87 W/kg) the extent of the RF-field induced hyperthermia was within the measurement accuracy (+/-0.1 degrees C) of the temperature probes used in the tests. The field-induced temperature increase was greatest in the first week of incubation and was less pronounced in the last (third) week before hatching. In both the short- and the long-term exposures, the temperature of the exposed tissue and the amniotic fluid, respectively, reached its maximum (asymptotic) approximately 40-50 min after the RF field was switched on. After the field was switched off, the temperature inside the exposed eggs returned to its initial value within 40-50 min.
Modeling of a Deepwater Turbidite Reservoir Conditional to Seismic Data Using (2002)

Geological interpretation and seismic data analysis provide two complementary sources of information to model reservoir architecture. Seismic data affords the opportunity to identify geologic patterns and features at a resolution on the order of 10’s of feet, while well logs and conceptual geologic models provide information at a resolution on the order of one foot. Both the large-scale distribution of geologic features and their internal fine-scale architecture influence reservoir performance. Development and application of modeling techniques that incorporate both large-scale information derived from seismic and fine-scale information derived from well logs, cores, and analog studies represents a significant opportunity to improve reservoir performance predictions. In this paper we present a practical new geostatistical approach for solving this difficult data integration problem and apply it to an actual, prominent reservoir. Traditional geostatistics relies upon a variogram to describe geologic continuity. However, a variogram, which is a two-point measure of spatial variability, cannot describe realistic, curvilinear or geometrically complex patterns. Multiple-point geostatistics uses a training image instead of a variogram to account for geological information. The training image provides a conceptual description of the subsurface geological heterogeneity, containing possibly complex multiple-point patterns of geological heterogeneity. Multiple-point statistics simulation then consists of anchoring these patterns to well data and seismic-derived information. This work introduces a novel alternative approach to traditional Bayesian modeling to incorporate seismic. The focus in this paper lies in demonstrating the practicality, flexibility and CPU-advantage of this new approach by applying it to an actual deep-water turbidite reservoir. Based on well log interpretation and a global geological understanding of the reservoir architecture, a training image depicting sinuous sand bodies is generated using a non-conditional object-based simulation algorithm. Disconnected sand bodies are interpreted from seismic amplitude data using a principal component cluster analysis technique. In addition, a map of local sand probabilities obtained from a principal component proximity transform of the same seismic is generated. Multiple-point geostatistics then simulates multiple realizations of channel bodies constrained to the local sand probabilities, partially interpreted sand bodies and well-log data. The CPU-time is comparable to traditional geostatistical methods.