gebhart/patrepeval huggingface dataset:
Identification of G‐protein binding sites of the human interleukin‐8 receptors by functional mapping of the intracellular loops (1996)

Interleukin 8 (IL-8) is considered to be a major mediator of the inflammatory response. Recent evidence indicates that a direct physical association occurs between IL-8 receptors and the alpha subunit of guanine nucleotide regulatory protein (Gi(alpha)2) upon stimulation of human neutrophils by IL-8. In the present study, we identified by site-directed mutagenesis key residues within the three intracellular loops of the IL-8RA receptor involved in the interaction with Gi(alpha)2. We first systematically mutated, in groups of two to four, all the residues in the three intracellular loops of the IL-8 type A receptor to alanine and analyzed the mutant receptors transiently expressed in 293 cells. Four residues in the second intracellular loop (Y136, L137, I139, V140) and one residue in the third intracellular loop (M241) were shown to be crucial for mediating calcium signaling in response to IL-8. Other residues in the second and third intracellular loops were also found to affect IL-8RA-mediated signaling, but to a lesser extent. These effects were not due to lower expression or low IL-8 binding affinities to the mutated receptors. Mutagenesis of the residues in the first intracellular loop had only weak effects on the mobilization of calcium induced by IL-8. We then used a coimmunoprecipitation protocol with anti-Gi(alpha)2 antibodies to determine the involvement of the two regions defined above in Gi(alpha)2 coupling to IL-8 type A receptors. Whereas the anti-Gi(alpha)2 antibodies coimmunoprecipitated IL-8 receptors in the wild-type cells, this interaction was lost in cells expressing mutated receptors that affected intracellular calcium mobilization. The peptides corresponding to the regions of the type A receptor found to be critical for Gi(alpha)2 coupling and induction of intracellular calcium mobilization were next introduced into cells expressing wild-type IL-8RA or IL-8RB to assess their role in coupling Gi(alpha)2 to both IL-8 receptors. The results obtained in the latter experiments suggest that the same regions of the second intracellular loop (Y136, L137, I139, V140) and of the third intracellular loop (M241) are critically involved in the coupling of both IL-8RA and IL-8 RB to Gi(alpha)2 as well as to a downstream effector (or effectors) involved in calcium mobilization.
Peripheral T‐cell lymphomas, unspecified (or not otherwise specified): a review (2007)

Peripheral T-cell lymphomas (PTCL) comprises a heterogeneous group of haematological tumours, which originate from mature T-cells, and constitute less than 15% of all non-Hodgkin's lymphomas (NHLs) in adults. The current WHO classification recognizes nine distinct clinicopathologic peripheral T-cell NHLs, being the 'unspecified variant' (PTCL-U) the most common subtype. These neoplasms often present in advanced stage at diagnosis, and most commonly have an aggressive clinical course requiring prompt treatment. The rarity of these tumours requires additional studies to better understand their biology and search for new therapies which may hopefully improve the dismal outcome of most patients. This review aims to describe the pathobiological aspects as well the clinical characteristics and current therapeutic strategies of the PTCLs, with special attention to the group of PTCL-U.
An authentication framework for web access to remote hosts (1999)

An authentication framework is described that provides a secure means for clients to access remote computing resources via the Web. Clients authenticate themselves to a proxy Web server using a secure protocol and a digital certificate. The server constructs a fingerprint (digest) of the certificate using a secure hash function. This hash value acts as a user's identification, which is used to obtain remote login information for that user from an authentication database. Client commands can then be executed by the Web server on remote hosts using the Secure Shell protocol. The system does not require a large amount of specialized software, and creates a secure, encrypted chain between the client and any number of remote resources.
Constitutive expression of TGF-bêta1, interleukin-6 and interleukin-8 by tumor cells as a major component of immune escape in human ovarian carcinoma. (2004)

Tumors could use several mechanisms to coexist with the host's immune system or to protect themselves from an immune response. Thus, insufficient expression of cell surface molecules on tumor cells, which are important for T cell recognition or activation, could lead to induction of a state of tolerance. Tumor cells could also produce cytokines that would inhibit the immune response and allow tumor progression. Here, we studied, in vitro, the cell surface expression of immunologically important molecules in seven ovarian carcinoma (OVCA) cell lines and the constitutive expression of cytokines. All OVCA cell lines expressed MHC class I molecules, ICAM-1 and LFA-3 adhesion molecules, necessary to induce a specific cytotoxic T-cell response, as well as the CD40 costimulatory molecules. Conversely, the lack of the dominant costimulatory molecules, CD80 (B7.1) and CD86 (B7.2) could be a possible explanation of poor immunogenicity of OVCA tumors. Immunosuppressive TGF-beta1 was detected at the mRNA level in all cell lines but was weakly secreted in supernatants. By contrast, IL-10 was never found. Most of them constitutively produced IL-8 and IL-6, two cytokines known as tumor promoting factors whereas the proinflammatory cytokines TNF-alpha, IL-1beta and GM-CSF were rarely produced. Data from this study could be useful for designing new strategies of immunotherapy to improve immunogenicity and/or limit protumor cytokine production.
ChemInform Abstract: Substituted Vinyl Azides in Synthesis of Furo(3,2‐b:4,5‐b′)dipyrroles and Pyrrolo(2′,3′:4,5)furo(3,2‐c)pyridines. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Activated Conformations of Very Late Activation Integrins Detected by a Group of Antibodies (HUTS) Specific for a Novel Regulatory Region(355-425) of the Common β1 Chain (1996)

The very late activation antigens (VLA) or beta 1 integrins mediate cell attachment to different extracellular matrix proteins and intercellular adhesions. The ligand binding activity of these adhesion receptors is not constitutive and can be regulated by temperature, presence of extracellular divalent cations, stimulatory monoclonal antibodies (mAbs), and cellular activation. We have generated three novel mAbs, HUTS-4, HUTS-7, and HUTS-21, recognizing specific epitopes on the common beta 1 subunit (CD29) of VLA integrins whose expression correlates with the ligand binding activity of these heterodimeric glycoproteins. This correlation has been demonstrated for several integrin heterodimers in different cell systems using a variety of extracellular and intracellular stimuli for integrin activation. Thus, the presence of micromolar concentrations of extracellular Mn2+, preincubation with the activating anti-beta 1 mAb TS2/16, and cell treatment with phorbol esters or calcium ionophores, induced the expression of the HUTS beta 1 epitopes on T lymphoblasts. Using a panel of human-mouse beta 1 chimeric molecules, we have mapped these epitopes to the 355-425 sequence of the beta 1 polypeptide. This segment represents therefore a novel regulatory region of beta 1 that is exposed upon integrin activation. Interestingly, binding of HUTS mAbs to partially activated VLA integrins results in maximal activation of these adhesion receptors and enhancement of cell adhesion to beta 1 integrin ligands collagen, laminin, and fibronectin.
Synthèse de flavones et de xanthones dans la série du benzo[4,5]thiophène (1978)

Abstract Nous décrivons les premières synthèses d'aryl‐2 benzo[4,5]thiéno[2,3‐ b ]pyrannone‐4 et d'aryl‐2 benzo[4,5]thiéno[3,2‐ b ] pyrannone‐4 à partir du benzo[4,5]thiophène, et étendons ces cyclisations pour obtenir des hétérologues des xanthones.
Theory Of Elasticity (1951)

Chapter 1: Stresses and Strains Chapter 2: Foundations of Plasticity Chapter 3: Elasto-Plastic Bending and Torsion Chapter 4: Plastic Analysis of Beams and Frames Chapter 5: Further Solutions of Elasto-Plastic Problems Chapter 6: Theory of the Slipline Field Chapter 7: Steady Problems in Plane Strain Chapter 8: Non-Steady Problems in Plane Strain
Analysis of wound-induced gene expression in Nicotiana species with contrasting alkaloid profiles (2004)

We determined the capacity of three Nicotiana (Solanaceae) species with very different alkaloid profiles (Nicotiana sylvestris Speg & Comes, Nicotiana alata Link & Otto and Nicotiana glauca Grah.) to increase their alkaloid contents in both leaf and root tissues following foliage damage. We also investigated the transcriptional responses of genes encoding enzymes important for alkaloid biosynthesis, namely quinolinate phosphoribosyltransferase (QPT), putrescine N-methyltransferase (PMT), ornithine decarboxylase (ODC) and the putative alkaloid biosynthetic gene A622. In response to wounding of foliage in the well studied 'model' species N. sylvestris, a rise, approximately 2-fold, in leaf nicotine levels was observed several days after a 4-5-fold increase in the transcript levels of all genes in the roots. In contrast, leaf tissues of the ornamental tobacco N. alata showed very low levels of any pyridine alkaloid, even when analysed 1 week after wounding, correlating with a general lack of transcript abundance representing any of these genes in leaves or roots following foliage damage. However, addition of methyl jasmonate to cultured roots of N. alata did produce elevated levels of nicotine and anatabine raising the possibility that components of the leaf-root wound signalling system in N. alata are different from those in N. sylvestris. Wounding of the tree tobacco N. glauca, was followed by a 2-fold increase in anabasine levels several days later. This increase followed a large rise in transcript levels of ODC, QPT and A622, though not PMT, in wounded leaves, but not in non-wounded leaves or roots. These data support the hypothesis that N. glauca is able to produce increased anabasine levels following wounding in its foliage, setting it apart from N. sylvestris where induced alkaloid production takes place in roots. We discuss the possibility that increased transcript levels detected by ODC and A622 probes play important roles in anabasine synthesis in N. glauca.
Oncogenic activation of p185neu stimulates tyrosine phosphorylation in vivo. (1988)

p185, the product of the neu/erbB2 proto-oncogene, is oncogenically activated by a point mutation that substitutes glutamic acid for valine in the transmembrane domain of the protein. We have found that the transforming form of p185 differs from its normal counterpart in inducing increased tyrosine phosphorylation of other proteins in vivo and in having a much shorter half-life. These results support the model that the transforming p185 resembles a ligand-activated receptor.
Prognostic value of biochemical markers, 12‐lead ECG and patient characteristics amongst patients calling for an ambulance due to a suspected acute coronary syndrome (2004)

Amongst patients with a clinical suspicion of acute coronary syndrome, those with the combination of ECG signs of myocardial ischaemia and the elevation of any biochemical marker on arrival of the ambulance form a group with a particularly high risk of death. However, age as well as aspects of clinical history and type of symptoms independently contribute to prognostic information.
Titanium Complexes of Silicon-Bridged Cyclopentadienyl−Phenoxy Ligands Modified with Fused-Thiophene: Synthesis, Characterization, and Their Catalytic Performance in Copolymerization of Ethylene and 1-Hexene (2009)

Titanium complexes of silicon-bridged cyclopentadienyl−phenoxy ligands modified with fused-thiophene, R2Si(L)(3-tBu-5-Me-2-C6H2O)TiCl2 {5: R = Me; L = 6-L1; L1 = 2,4,5-Me3-cyclopenta[2,3-b]thiophenyl, 6: R = Et; L = 6-L1, 9a: R = Me; L = 4-L2; L2 = 2,5-Me2-cyclopenta[3,2-b]thiophenyl, 10a: R = Et; L = 4-L2, 16: R = Me; L = 7-L3; L3 = 2,5-Me2-cyclopenta[1,2-b:4,3-b′]dithiophenyl, 17: R = Et; L = 7-L3, 18: R = Et; L = 7-L4; L4 = cyclopenta[1,2-b:4,3-b′]dithiophenyl, 19: R = Et; L = 4-L5; L5 = 2,6-Me2-cyclopenta[2,1-b:3,4-b′]dithiophenyl, 20: R = Et; L = 4-L6; L6 = cyclopenta[2,1-b:3,4-b′]dithiophenyl}, were prepared by treating TiCl4 with the dilithium salts generated by reaction of the corresponding ligands (3, 4, 7, 8, and 11−15) with nBuLi in the presence of Et3N in toluene. In the ligand synthesis, the use of L2-H afforded R2Si(L-H)[2-(OCH2CH═CH2)-3-tBu-5-Me-C6H2] (7a: R = Me; L = 4-L2, 8a: R = Et; L = 4-L2) along with a small amount of the isomer R2Si(L-H)[2-(OCH2CH═CH2)-3-tBu-5-Me-C6H2] (7b: R = Me; L = 6-L2, 8b: R = Et; L = 6-L2), respectively. X-ray crystallographic studies of 6, 17, and 19 indicate that the cyclopentadienyl moiety of 6 with TFCp (TFCp = thiophene-fused cyclopentadienyl) is bound to the titanium atom in an η5-fashion, whereas the coordination of the moieties to the metal in 17 and 19 with DTFCp (DTFCp = dithiophene-fused cyclopentadienyl) contributes in somewhat of an η3-manner. The newly prepared complexes 5, 6, 9, 10, and 16−20 exhibited high catalytic activities for copolymerization of ethylene and 1-hexene in the presence of iBu3Al/[Me2NHPh][B(C6F5)4] and afforded higher molecular weight copolymer than the parent tetramethyl-substituted cyclopentadienyl complex Me2Si(η5-C5Me4)(3-tBu-5-Me-2-C6H2O)TiCl2 (1a). The complexes 6, 17, and 19 were thermally stable and exhibited high polymerization activities, even at 180 °C. Among tested complexes, the DTFCp complexes 17 and 19 at 180 °C showed very high activity and produced high molecular weight copolymers with good 1-hexene content.
Molecular Genetics of Pathogenesis by Soft-Rot Erwinias (1987)

The soft-rot erwinias are enterobacteria able to cause diseases, a symptom of which is the maceration of host tissues, on a wide range of plants. In the past, this group of pathogens was grouped into a single species, Erwinia caroto­ vora, divided into subspecies (129). Now, the group is split into two main species, Erwinia chrysanthemi and E. carotovora, the latter being divided into the three subspecies, carotovora, atroseptica, and betavasculorum (89). Two other minor pathogens with restricted host range are included in the soft-rot group: Erwinia cypripedii and Erwinia rhapontici (108). The general characteristics and the ecology of these bacteria have been previously re­ viewed (107, 108, 129). The soft-rot erwinias can infect plants in culture as well as in storage. Besides soft rot itself, other symptoms may be observed on the growing plant. For instance, E. chrysanthemi induces a wilt on carnation. E. carotovora subsp. atroseptica, E. carotovora subsp. carotovora, and E. chrysanthemi can cause blackleg on potato, which has leaf wilting as a symptom (107, 108). Infection of chrysanthemu?l by E. chrysanthemi generally induces a canker on the stem, starting from the crown (25). In plants that reproduce vegetative­ ly, the infection can be systemic and, when conditions for development of the disease are not met, latent. Host specificity, although not as strict as that observed in plant-pathogenic pseudomonads and xanthomonads (78, 130), seems to occur in E. chrysanthemi strains. It has been proposed that E. chrysanthemi be divided into five pathotypes (51, 52). Soft-rot erwinias produce several extracellular enzymes that can attack components of the plant
The impact of switching strategies on power quality for integral cycle controllers (2003)

Integral cycle controllers (ICCs) are used for load power control. ICCs are switched at an integral number of cycles to effect this control. The load current for such controllers has an impact on the distribution supply bus: the impact occurs for low frequency (subsynchronous) phenomena and high frequency (supersynchronous) phenomena. Subsynchronous impact includes flicker on the distribution bus, and supersynchronous phenomena includes supply transformer core loss. The management of these power quality issues is discussed in terms of selecting the controller switching strategy. An innovative concept of group frequencies is discussed.
Passive and Active Inspired Gas Humidification in Infants and Children (1989)

The hypothesis that both active and passive airway humidification prevents hypothermia in infants and children, but that neither decreases the duration of postoperative recovery was tested. Twenty-seven ASA physical status 1 or 2 patients were studied who weighed between 5 and 30 kg, underwent superficial operations, were anesthetized with halothane and 70% N2O, and whose lungs were ventilated via a Rees modification of an Ayre's t-piece. The children were randomly assigned to receive active airway humidification and warming using an MR450 Servo airway heater and humidifier set at 37 degrees C (n = 10), passive airway humidification using the Humid-Vent 1 heat and moisture exchanger placed between the Ayre's t-piece and the endotracheal tube (n = 8), or no airway humidification and heating (control, n = 9). Distal tracheal and tympanic membrane temperatures and airway humidity were recorded during the first 90 min of surgery. Rectal temperature was measured during the postanesthetic recovery period. Relative humidity of inspired respiratory gases was approximately 30% in the control group and approximately 90% in the group given active airway humidification. Initial inspired humidity in the passive humidification group (50%) increased to approximately 80%, a level not significantly different from that in the active group after 80 min of anesthesia. Central body temperature increased 0.25 degrees C during active active airway humidification and heating, whereas temperature decreased 0.25 degrees C during passive humidification and 0.75 degrees C without airway humidification. Distal tracheal temperature was significantly higher in the groups given passive and active humidification than in the control group.(ABSTRACT TRUNCATED AT 250 WORDS)
Surface Parameterization for Meshing by Triangulation Flattenin (2000)

We propose a new method to compute planar triangulations of triangulated surfaces for surface parameterization. Our method computes a projection that minimizes the distortion of the surface metric structures (lengths, angles, etc.). It can handle any manifold surface, including surfaces with large curvature gradients and non-convex domain boundaries. We use only the necessary and sufficient constraints for a valid two-dimensional triangulation. As a result, the existence of a theoretical solution to the minimization procedure is guaranteed.
Places we love: The city of Bendigo, Victoria (2003)

'Places we love' is a new column that provides glimpses of some of Australia's Art Deco treasures. The first in the series is Bendigo, one of Victoria's major cities, located about 120 km from Melbourne.
Antibodies to synthetic peptides from the tubulin regulatory domain interact with tubulin and microtubules. (1988)

The carboxyl-terminal region of tubulin alpha and beta subunits plays a major role in regulating its assembly into microtubules and constitutes an essential domain for the selective interaction of microtubule-associated proteins (MAPs). With the goal of understanding the structural basis of the regulatory function of the carboxyl-terminal domains of tubulin subunits, we have produced rabbit antisera against two MAP-interacting peptides Lys-Asp-Tyr-Glu-Glu-Val-Gly-Val-Asp-Ser-Val-Glu of alpha-tubulin and Tyr-Gln-Gln-Tyr-Gln-Asp-Ala-Thr-Ala-Asp-Glu-Gln-Gly of beta subunit. The affinity-purified alpha and beta anti-peptide antibodies interacted specifically with tubulin and with the respective peptide antigens but did not interact with MAPs. Substoichiometric amounts of both antibodies showed the capacity to inhibit in vitro MAP-induced tubulin assembly and to promote a fast depolymerization of preassembled microtubules. Taxol-promoted assembly of pure tubulin was not inhibited by the antibodies. In the presence of MAP-2 and taxol, the antibodies decreased the MAP-2 content of taxol-promoted microtubules. The interaction with microtubules was corroborated by immunofluorescence experiments in HeLa and NE-18 lung carcinoma cells. The epitopes recognized by the alpha and beta anti-peptide antibodies appear to be located in the outer surface of the microtubular structure.
ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. (1990)

We have cloned, sequenced, and expressed full length cDNA clones encoding two abundant, luminal endoplasmic reticulum proteins (ERp), ERp59/PDI and ERp72. ERp59/PDI has been identified as the microsomal enzyme protein disulfide isomerase (PDI). An analysis of the amino acid sequence of ERp72 showed that it shared sequence identity with ERp59/PDI at three discrete regions, having three copies of the sequences that are thought to be the CGHC-containing active sites of ERp59/PDI. Thus, ERp72 appears to be a newly described member of the family of CGHC-containing proteins. ERp59/PDI has the sequence KDEL at its COOH terminus while ERp72 has the related sequence KEEL. Removal of the KDEL of ERp59/PDI or the KEEL of ERp72 by in vitro mutagenesis techniques and subsequent analysis of the mutants in transient expression assays, showed that both sequences are endoplasmic reticulum retention signals for their respective proteins. The most dramatic difference in secretion between the wild type and the mutant forms of the protein was seen in the case of ERp72.
Radiation effects on circulating and endothelial cell interactions studied by quantitative real-time videomicroscopy. (2002)

The results of an in vitro flow assay with whole blood showed that under physiological flow conditions, irradiation affected the function of EC; pro-inflammatory and thrombogenic responses were enhanced, which may contribute to in vivo radiation-induced vascular occlusion and fibrosis.
Seroreactivity to microbial components in Crohnʼs disease is associated with ileal involvement, noninflammatory disease behavior and NOD2/CARD15 genotype, but not with risk for surgery in a Hungarian cohort of IBD patients (2007)

Serological markers were useful in the differentiation between CD and UC in an Eastern European IBD cohort. Reactivity to microbial components was associated with disease phenotype and NOD2/CARD15 genotype, further supporting the role of altered microbial sensing in the pathogenesis of CD.
A mode-evolution-type integrated-optical beam combiner for coherent receivers (1991)

An integrated-optic sum-and-difference optical beam combiner for coherent receivers based on adiabatic mode evolution in an asymmetric waveguide junction is described. The device was packaged as a 2*2 fiber-compatible LiNbO/sub 3/ chip, and operated as a 180 degrees optical hybrid for combining arbitrary input polarization, exhibiting excellent balance and moderate loss. The advantage of this component over previously studied directional coupler beam combiners is the absence of a strict interaction length requirement for obtaining balanced separation. Integration of this component with a passive mode-evolution-type polarization splitter will result in polarization-diversity receivers with broad constraints on processing, design, and wavelength of operation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>