gebhart/patrepeval huggingface dataset:
Amino acid digestibility and energy content of deoiled (solvent-extracted) corn distillers dried grains with solubles for swine and effects on growth performance and carcass characteristics1,2 (2011)

A study with 3 experiments was conducted to determine the AA digestibility and energy concentration of deoiled (solvent-extracted) corn distillers dried grains with solubles (dDGS) and to evaluate its effect on nursery pig growth performance, finishing pig growth performance, and carcass traits. In Exp. 1, a total of 5 growing barrows (initial BW = 30.8 kg) were fitted with a T-cannula in the distal ileum and allotted to 1 of 2 treatments: 1) a diet with dDGS as the sole protein source, or 2) a N-free diet for determining basal endogenous AA losses in a crossover design at 68.0 kg of BW. Apparent and standardized (SID) ileal digestibility of AA and energy concentration of dDGS were determined. In Exp. 2, a total of 210 pigs (initial BW = 9.9 kg) were used in a 28-d experiment to evaluate the effect of dDGS on nursery pig performance. Pigs were allotted to 5 dietary treatments (0, 5, 10, 20, or 30% dDGS) formulated to contain equal ME (increased added fat with increasing dDGS) and SID Lys concentrations based on the values obtained from Exp. 1. In Exp. 3, a total of 1,215 pigs (initial BW = 29.6 kg) were used in a 99-d experiment to determine the effect of dDGS on growth and carcass characteristics of finishing pigs. Pigs were allotted to dietary treatments similar to those used in Exp. 2 and were fed in 4 phases. The analyzed chemical composition of dDGS in Exp. 1 was 35.6% CP, 5.29% ash, 4.6% fat, 18.4% ADF, and 39.5% NDF on a DM basis. Apparent ileal digestibility values of Lys, Met, and Thr in dDGS were 47.2, 79.4, and 64.1%, respectively, and SID values were 50.4, 80.4, and 68.9%, respectively. The determined GE and DE and the calculated ME and NE values of dDGS were 5,098, 3,100, 2,858, and 2,045 kcal/kg of DM, respectively. In Exp. 2, nursery pig ADG, ADFI, and G:F were similar among treatments. In Exp. 3, increasing dDGS reduced (linear; P < 0.01) ADG and ADFI but tended to improve (linear; P = 0.07) G:F. Carcass weight and yield were reduced (linear; P < 0.01), loin depth tended to decrease (linear; P = 0.09), and carcass fat iodine values increased (linear; P < 0.01) as dDGS increased. No difference was observed in backfat, percentage of lean, or fat-free lean index among treatments. In conclusion, dDGS had greater CP and AA but less energy content than traditional distillers dried grains with solubles. In addition, when dietary fat was added to diets to offset the reduced ME content, feeding up to 30% dDGS did not affect the growth performance of nursery pigs but did negatively affect the ADG, ADFI, and carcass fat quality of finishing pigs.
P.10: WITHDRAWN: P.11: Recognition of Existence of n‐type IGZO Layer in CAAC‐IGZO Film under Source and Drain Electrode Made of Tungsten (2013)

Abstract In a C‐axis aligned crystal (CAAC) IGZO TFT, contact between a CAAC‐IGZO film and source and drain electrode was good. In this study, we recognized the existence of n‐type IGZO layer serving as contact layer by a novel experimental method where a depth profile of the sheet resistance.
Design and Synthesis of a Fluorescent Reporter of Protein Kinase Activity (2002)

There is widespread interest in developing fluorescent reporters of protein kinase activity, species that can furnish a visual readout of both when and where intracellular kinases are activated in response to a stimulus. We have constructed and identified, via a combination of rational design, library synthesis, and screening, a difluorofluorescein-appended peptide-based species that responds to protein kinase C phosphorylation in a fluorescently sensitive fashion. The phosphorylation-induced divalent metal ion-mediated 265% enhancement in fluorescence proceeds with a V(max) of 8.5 micromol/min.mg and a K(m) of 20.5 microM.
Molecular pathways in myocardial development: a stem cell perspective (2003)

The heart has long been considered to adapt to increased work or pathology through the cellular growth process of hypertrophy. However, recent evidence for the existence of endogenous stem cells and regenerative capacity in the adult heart has given new impetus to the quest for cell therapies for heart failure, which remains the number one killer in Western cultures. The molecular cues driving cardiac development are now being explored in detail and will come into sharp focus as regimes for stem cell differentiation and efforts to augment endogenous regeneration are trialed. This review briefly outlines the current state of knowledge on the molecular basis of the four modalities of myogenesis that have been identified in the developing vertebrate heart. Stem cell-mediated myogenic repair in the heart represents a fifth modality, and an exciting frontier with basic and practical implications that remain to be explored.
Microwave solid state circuit design (1988)

Preface. Introduction (P. Pramanick & P. Bhartia). Transmission Lines and Lumped Elements (I. Bahl). Resonators (A. Sharma & A. Khanna). Impedance Transformation Techniques (P. Shastry). Hybrids and Couplers (P. Bhartia & P. Pramanick). Filters (E. Griffin & I. Bahl). Active Devices (R. Trew). Passive Devices (R. Trew). Oscillators (A. Khanna). Amplifiers (I. Bahl & E. Griffin). Detectors and Mixers (R. Harrison). Microwave Control Circuits (K. Gupta). Frequency Multipliers and Dividers (R. Harrison). RF MEMS Devices and Circuit Applications (R. Ramadoss & K. Gupta). Circuit Fabrication Technologies (I. Bahl). Appendix A: Units and Symbols. Appendix B: Physical Constants and Other Data. Appendix C: ABCD and S-Parameters. Appendix D: Transfer Function Responses. Index.
Flow of information in the light-triggered cyclic nucleotide cascade of vision. (1981)

Photolyzed rhodopsin catalyzes the exchange of GTP for FDP bound to a protein in retinal rod outer segments. We previously proposed that the GTP complex of this protein regulates the cyclic GMP phosphodiesterase and that it may be the first amplified intermediate in visual excitation [Fung, B. K.-K. & Stryer, L. (1980) Proc. Natl. Acad. Sci. USA 77, 2500-2504]. We report here the identification and characterization of transducin, a regulatory protein consisting of three kinds of polypeptide chains: T alpha (39 kilodaltons), T beta (36 kilodaltons), and T gamma (approximately 10 kilodaltons). Reconstituted membranes containing transducin and rhodopsin but no phosphodiesterase exhibit GTPase activity and amplified binding of guanosine 5'[beta, gamma-imido]triphosphate (p[NH]ppG), a nonhydrolyzable analog of GTP, on illumination. A single photolyzed rhodopsin molecule led to the uptake of p[NH]ppG by 71 molecules of transducin. High-pressure liquid chromatography showed that the binding site for GTP is on the alpha subunit of transducin. The isolation of the complex of ;[NH]ppG with T alpha enabled us to determine whether this species is the activator of the phosphodiesterase. We found that phosphodiesterase on unilluminated disc membranes can indeed be fully activated by addition of T alpha containing bound p[NH]ppG. These findings strongly suggest that transducin is the first amplified information-carrying intermediate in the cyclic nucleotide cascade of vision.
Using Ground-Penetrating Radar to Delineate Fractures in the Rus Formation, Dammam Dome, Eastern Saudi Arabia (2004)

Ground-penetrating radar (GPR) has been used recently to detect many near-surface geological features in arid regions. In this study, GPR is used to delineate near-surface fractures within the Dammam Dome. This anticlinal structure covers ~160 km2 and hosts the first oil well discovered in Saudi Arabia. Doming is due to the buoyancy of the Hormuz Salt. Rock units present within the dome range in age from Paleocene to middle Miocene. Well-developed fractures crosscut the dome and extend for several km. Our results prove that GPR techniques can be used successfully to map precisely near-surface open and filled fractures. This technique is demonstrably useful for environmental, engineering, and geological studies.
Biotechnology Products and Their Development (2002)

This chapter contains sections titled: Regulatory Considerations Biotechnology vs. Conventional Drug Products Manufacturing Issues Product Classes and Resultant Clinical Trial Issues Safety Issues in Product Development Regulatory Issues Cell and Tissue Products Ethical Issues Informed Consent Acknowledgments References Appendix Biotechnology Industry Patents
The type-specific polysaccharides of Streptococcus suis. (1978)

Streptococcus suis types 1 and 2 were subjected to digestion with lysozyme. Serologically type-specific capsular polysaccharides were isolated from the lysates by ethanol precipitation followed by Sepharose 6B chromatography. The purified type 1 polysaccharide has a Kd value of 0.074 on a Sepharose 4B column and contains galactose, glucose, N-acetyl glucosamine, N-acetyl galactosamine, and sialic acid in a molar ratio of 2.42:1.00:1.00:1.13:1.39. The type 2 polysaccharide has a Kd value of 0.185 and is composed of rhamnose, galactose, glucose, N-acetyl glucosamine, and sialic acid in a molar ratio of 1.07:3.17:1.00:0.94:1.00. A comparison is drawn between the type polysaccharides of S. suis and those of group B streptococci.
5‐Fluorouracil permits access to a primitive subpopulation of peripheral blood stem cells (1993)

Peripheral blood stem cells (PBSC) contain a mixture of mature and immature hematopoietic progenitors. Resistance to 5-Fluorouracil (5-FU) has been used to identify and characterize primitive quiescent stem cells among bone marrow (BM) cells. To see if the same technique could be used to isolate a similar population of cells among PBSC, low-density peripheral blood mononuclear cells (PBMNC) were collected by cytapheresis in the regenerative phase after high-dose chemotherapy from patients with hematological malignancies. These PBMNC were incubated with increasing concentrations of 5-FU for 24 h. The viable 5-FU resistant cells were then cultured in semi-solid media in the presence of either single cytokines: TCM 5637, Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), or a combination of cytokines: interleukin 1 (IL-1) IL-1 + IL-3 + 5637, IL-1 + IL-3 + Stem Cell Factor (SCF). Low concentrations (5-10 micrograms/ml 5-FU) eliminated mature day 7 Colony Forming Units-Granulocyte Macrophage (CFU-GM) and spared day 7 clusters while enriching for day 14 CFU-GM, irrespective of the growth factors used. Higher concentrations of 5-FU (15, 20, 25 micrograms/ml) selected for later forming clonogenic elements. A combination of synergistic growth factors was required for the development of morphologically identifiable clonogenic elements resistant to 25 micrograms/ml 5-FU at day 21 of culture. Further experimentation demonstrated that SCF could effectively replace TCM 5637 in the cytokine combination for the detection of primitive late forming clonogenic elements. The presence of SCF potentiated colony formation by 5-FU resistant PBMNC. It was confirmed that GM-CSF alone was unable to support colony formation by PBMNC resistant to 25 micrograms/ml. These observations demonstrate that PBSC contain a heterogenous mixture of hematopoietic progenitors and that incubation with 25 micrograms/ml 5-FU permits access to a quiescent primitive stem cell population that requires a combination of synergistic growth factors for the development of morphologically identifiable clonogenic elements at day 21. Taken together, these results suggest that PBSC have similar characteristics to BM derived stem cells.
Head Group Analogs of Arachidonylethanolamide, the Endogenous Cannabinoid Ligand (1996)

Several analogs of an endogenous cannabimimetic, arachidonylethanolamide (anandamide), were synthesized to study the structural requirements of the ethanolamide head group. CB1 receptor affinities of the analogs were evaluated by a standard receptor binding assay using tritiated CP-55,940 as the radioligand and compared to anandamide which was shown to have a Ki of 78 nM. Replacement of the amide carbonyl oxygen by a sulfur atom had a detrimental effect on the CB1 affinity. The thio analogs of both anandamide and (R)-methanandamide showed very weak affinity for CB1. The secondary nature of the amidic nitrogen was also shown to be important for affinity, indicating a possible hydrogen-bonding interaction between the amide NH and the receptor. Introduction of a phenolic moiety in the head group resulted in the loss of receptor affinity except when a methylene spacer was introduced between the amidic nitrogen and the phenol. A select group of analogs were also tested for their affinity for the CB2 receptor using a mouse spleen preparation and were found to possess low affinities for the CB2 sites. Notably, anandamide and (R)-methanandamide demonstrated high selectivity for the CB1 receptor. Overall, the data presented here show that structural requirements of the head group of anandamide are rather stringent.
Demyelinating diseases (2006)

A diagnosis of demyelination carries important therapeutic and prognostic implications. In most cases the diagnosis is made clinically, and involvement of the histopathologist is largely confined to postmortem confirmation and clinicopathological correlation. However, every now and then, accurate diagnosis of the presence or cause of demyelination before death hinges on the histopathological assessment. Recognition of demyelination depends on an awareness of this as a diagnostic possibility, and on the use of appropriate tinctorial and immunohistochemical stains to identify myelin, axons and inflammatory cells. In biopsy specimens, the critical distinction is usually from ischaemic or neoplastic disease, and the types of demyelinating disease most likely to be encountered are multiple sclerosis, acute-disseminated encephalomyelitis, progressive multifocal leucoencephalopathy and extrapontine myelinolysis. Interpretation of the pathology has to be made in the context of the clinical, radiological and biochemical findings. Freezing of a small amount of fresh tissue allows for later virological studies, and electron microscopy is occasionally helpful for demonstration of viral particles.
Recombination-Mediated Genetic Engineering of a Bacterial Artificial Chromosome Clone of Modified Vaccinia virus Ankara (MVA) (2008)

The production, manipulation and rescue of a bacterial artificial chromosome clone of Vaccinia virus (VAC-BAC) in order to expedite construction of expression vectors and mutagenesis of the genome has been described (Domi & Moss, 2002, PNAS99 12415-20). The genomic BAC clone was 'rescued' back to infectious virus using a Fowlpox virus helper to supply transcriptional machinery. We apply here a similar approach to the attenuated strain Modified Vaccinia virus Ankara (MVA), now widely used as a safe non-replicating recombinant vaccine vector in mammals, including humans. Four apparently full-length, rescuable clones were obtained, which had indistinguishable immunogenicity in mice. One clone was shotgun sequenced and found to be identical to the parent. We employed GalK recombination-mediated genetic engineering (recombineering) of MVA-BAC to delete five selected viral genes. Deletion of C12L, A44L, A46R or B7R did not significantly affect CD8(+) T cell immunogenicity in BALB/c mice, but deletion of B15R enhanced specific CD8(+) T cell responses to one of two endogenous viral epitopes (from the E2 and F2 proteins), in accordance with published work (Staib et al., 2005, J. Gen. Virol.86, 1997-2006). In addition, we found a higher frequency of triple-positive IFN-gamma, TNF-alpha and IL-2 secreting E3-specific CD8+ T-cells 8 weeks after vaccination with MVA lacking B15R. Furthermore, a recombinant vaccine capable of inducing CD8(+) T cells against an epitope from Plasmodium berghei was created using GalK counterselection to insert an antigen expression cassette lacking a tandem marker gene into the traditional thymidine kinase locus of MVA-BAC. MVA continues to feature prominently in clinical trials of recombinant vaccines against diseases such as HIV-AIDS, malaria and tuberculosis. Here we demonstrate in proof-of-concept experiments that MVA-BAC recombineering is a viable route to more rapid and efficient generation of new candidate mutant and recombinant vaccines based on a clinically deployable viral vector.
Adaptive information retrieval: machine learning in associative networks (connectionist, free-text, browsing, feedback) (1986)

One interesting issue in artificial intelligence (AI) currently is the relative merits of, and relationship between, the "symbolic" and "connectionist" approaches to intelligent systems building. The performance of more traditional symbolic systems has been striking, but getting these systems to learn truly new symbols has proven difficult. Recently, some researchers have begun to explore a distinctly different type of representation, similar in some respects to the nerve nets of several decades past. In these massively parallel, connectionist models, symbols arise implicitly, through the interactions of many simple and sub-symbolic elements. One of the advantages of using such simple elements as building blocks is that several learning algorithms work quite well. The range of application for connectionist models has remained limited, however, and it has been difficult to bridge the gap between this work and st and ard AI. The AIR system represents a connectionist approach to the problem of free-text information retrieval (IR). Not only is this an increasingly important type of data, but it provides an excellent demonstration of the advantages of connectionist mechanisms, particularly adaptive mechanisms. AIR's goal is to build an indexing structure that will retrieve documents that are likely to be found relevant. Over time, by using users' browsing patterns as an indication of approval, AIR comes to learn what the keywords (symbols) mean so as use them to retrieve appropriate documents. AIR thus attempts to bridge the gap between connectionist learning techniques and symbolic knowledge representations. The work described was done in two phases. The first phase concentrated on mapping the IR task into a connectionist network; it is shown that IR is very amenable to this representation. The second, more central phase of the research has shown that this network can also adapt. AIR translates the browsing behaviors of its users into a feedback signal used by a Hebbian-like local learning rule to change the weights on some links. Experience with a series of alternative learning rules are reported, and the results of experiments using human subjects to evaluate the results of AIR's learning are presented.
High-Resistance Connection Detection in Induction Motor Drives Using Signal Injection (2013)

Two new methods for high-resistance connection (HRC) diagnosis in induction motor (IM) drives are presented in this effort. These automatic offline methods are based on a signal injection strategy applied to the IM at standstill. By measuring the voltage in the machine neutral point and phase currents, it is possible to detect and isolate connection problems. Experimental results showing a good sensitivity to HRCs and immunity to symmetrical stator resistance variations were obtained in a laboratory setup.
Inhibition of coronary artery reocclusion after thrombolysis with an RGD-containing peptide with no significant effect on bleeding time (1993)

TP9201 may be an effective therapy for the prevention of re-thrombosis after thrombolytic therapy without adversely affecting hemostasis.
Evoked Electromyographic Technique for Quantitative Assessment of the Innervation Status of Laryngeal Muscles (2005)

This evoked electromyographic technique could provide quantitative information regarding the extent of muscle innervation during denervation and regeneration in case of laryngeal paralysis.
Metabolic Modulation of Epigenetics in Gliomas (2013)

Cancer metabolism and epigenetics are two relatively new areas of cancer research. Recent years have seen an explosion of studies implicating either altered tumor metabolism or epigenetic mechanisms in the pathogenesis or maintenance of brain tumors. A new paradigm is emerging in cancer biology that represents a convergence of these themes, the metabolic regulation of epigenetics. We discuss this interrelationship in the context of two metabolic enzymes that can influence the pathogenesis of gliomas by altering the epigenetic state. The first of these enzymes is isocitrate dehydrogenase 1 (IDH1), which is mutated in secondary glioblastomas and ~70% of grade II/III astrocytomas and oligodendrogliomas. Mutant IDH1 results in the production of a metabolite 2-hydroxyglutarate (2-HG) that can inhibit DNA and histone demethylating enzymes resulting in the glioma-CpG island phenotype (G-CIMP) and increased histone methylation marks. Pyruvate kinase M2 (PKM2), an enzyme that plays a critical role in the glycolytic pathway, is a second example of a metabolic enzyme that can affect histone modifications. In epidermal growth factor receptor (EGFR)-driven glioblastoma, PKM2 translocates to the nucleus and phosphorylates histone 3 at threonine 11 (H3-T11). This causes dissociation of HDAC3 from the CCND1 (Cyclin D1) and c-MYC promoters and subsequent histone acetylation, leading to transcription of Cyclin-D1 and c-MYC, and subsequent cell proliferation. Modification of the epigenetic state by alterations in metabolic enzymes is a novel phenomenon that contributes to the pathogenesis of gliomas and may help in the identification of new therapeutic targets.
The Alzheimer's Disease Mitochondrial Cascade Hypothesis (2010)

We first proposed the mitochondrial cascade hypothesis of sporadic Alzheimer's disease (AD) in 2004. Our core assumptions were a person's genes determine baseline mitochondrial function and durability, this durability determines how mitochondria change with advancing age, and critical changes in mitochondrial function initiate other pathologies characteristic of AD. Since then several lines of investigation report data consistent with or supportive of our hypothesis. In particular, AD endophenotype studies suggest a strong maternal genetic contribution, and links between mitochondrial function, tau phosphorylation, and amyloid-beta (Abeta) amyloidosis are increasingly recognized. As predicted, AD therapies designed to reduce Abeta thus far have had at best very limited clinical benefits; our hypothesis identifies alternative therapeutic targets. While placing mitochondria at the apex of an AD cascade certainly remains controversial, it is increasingly accepted by the AD research community that mitochondria play an important role in the late-onset forms of the disease. Even if the mitochondrial cascade hypothesis proves incorrect, considering its assumptions could potentially advance our understanding of sporadic, late-onset AD.
A low speed, high-torque, direct-drive permanent magnet generator for wind turbines (2002)

There is a market for small, efficient and cost-effective wind generators for mini-grid and remote power systems. Direct-drive permanent magnet generators have become very attractive for this application. This paper describes the improvements achieved in an outer-rotor direct-drive permanent magnet generator by using finite element analysis and optimisation techniques. The starting torque of the generator is studied. An optimisation routine for the design, including magnetic finite element analysis and lumped-parameter thermal model, is presented. A prototype for 20 kW, 211 rpm generator was built. The test results with a resistive load confirm the satisfactory operation of the generator. Compared with the previous prototype, the new design has lower mass, lower starting torque and improved efficiency.