gebhart/patrepeval huggingface dataset:
""El mal-vivir en la" cultura light". Ofrendas tanáticas del adolescente actual (2008)

El sentimiento de mal-vivir resulta consustancial a la experiencia humana y consecuentemente es parte del quantum de displacer que acompana la subjetividad de cada epoca. El igualitarismo, al decir de Lacan, que el discurso del amo profiere, en tanto segrega el modo de satisfaccion de cada uno, constituye la «canallada moderna». La «cultura del bienestar» que caracteriza la epoca actual es quebrantada por el mal-vivir resultante de la irrupcion de desbordes tales como: a-dicciones (duras y blandas, a comer, a no comer); los intentos de suicidio y otros actos violentos. Lo ante dicho configura, a nuestro criterio, una ofrenda sacrificial del cuerpo al Otro y se erigiria como el ultimo recurso, la ultima defensa (aun a costa de la vida), para preservar algun espacio de subjetivacion. El adolescente, en tanto participa de una franja etarea que se caracteriza por constantes movimientos de transformacion para la consumacion subjetiva, es mas propenso a frustrarse y responder violentamente a los embates del contexto social. En este trabajo nos hemos propuesto revisitar algunas de las coordenadas que orientan los diferentes y novedosos modos del padecer humano y sus vicisitudes en la actualidad.
Modulation of plant growth by HD‐Zip class I and II transcription factors in response to environmental stimuli (2011)

Plant development is adapted to changing environmental conditions for optimizing growth. This developmental adaptation is influenced by signals from the environment, which act as stimuli and may include submergence and fluctuations in water status, light conditions, nutrient status, temperature and the concentrations of toxic compounds. The homeodomain-leucine zipper (HD-Zip) I and HD-Zip II transcription factor networks regulate these plant growth adaptation responses through integration of developmental and environmental cues. Evidence is emerging that these transcription factors are integrated with phytohormone-regulated developmental networks, enabling environmental stimuli to influence the genetically preprogrammed developmental progression. Dependent on the prevailing conditions, adaptation of mature and nascent organs is controlled by HD-Zip I and HD-Zip II transcription factors through suppression or promotion of cell multiplication, differentiation and expansion to regulate targeted growth. In vitro assays have shown that, within family I or family II, homo- and/or heterodimerization between leucine zipper domains is a prerequisite for DNA binding. Further, both families bind similar 9-bp pseudopalindromic cis elements, CAATNATTG, under in vitro conditions. However, the mechanisms that regulate the transcriptional activity of HD-Zip I and HD-Zip II transcription factors in vivo are largely unknown. The in planta implications of these protein-protein associations and the similarities in cis element binding are not clear.
Revisiting chordoma with brachyury, a "new age" marker: analysis of a validation study on 51 cases. (2010)

Exclusive brachyury expression in more than 90% of chordomas indicates its value as a unique, specific marker with other sensitive markers like cytokeratin, epithelial membrane antigen, and/or S100 protein in substantiating a diagnosis of chordoma, including on small biopsies.
Telepresence-the technology and its economic and social implications (2002)

Telepresence is the experience of being present at a real world location remote from one's own immediate physical environment. This is much more than simple teleconferencing since it attempts to allow the user to feel immersed in the remote environment and to be able, through teleoperation, to manipulate or control remote events. By using the Strathclyde Transparent Telepresence Research Group and the Strathclyde Telepresence System as examples the paper considers the organisation and technology that makes a telepresence system possible. It examines the current limitations of such systems and their eventual potential. The typical applications, present and future, of telepresence systems are noted before going on to discuss the economic and social implications of these applications. The paper is intended to stimulate further thought on possible future advantages and disadvantages of this emerging technology.
Development of RAF Quality 150mm 4H-SiC Wafer (2014)

We have developed RAF (Repeated a-face) growth method which is high quality bulk crystal growth technology [1, 2]. A block crystal more than 150 mm square size was produced by the RAF growth method. Since c-face growth crystal was produced on the seed obtained from the block crystal, high quality 150mm 4H-SiC wafer was achieved. This paper reports the results of the quality evaluation.
A PEST-Like Sequence in Listeriolysin O Essential for Listeria monocytogenes Pathogenicity (2000)

Establishment and maintenance of an intracellular niche are critical to the success of an intracellular pathogen. Here, the pore-forming protein listeriolysin O (LLO), secreted by Listeria monocytogenes, was shown to contain a PEST-like sequence (P, Pro; E, Glu; S, Ser; T, Thr) that is essential for the virulence and intracellular compartmentalization of this pathogen. Mutants lacking the PEST-like sequence entered the host cytosol but subsequently permeabilized and killed the host cell. LLO lacking the PEST-like sequence accumulated in the host-cell cytosol, suggesting that this sequence targets LLO for degradation. Transfer of the sequence to perfringolysin O transformed this toxic cytolysin into a nontoxic derivative that facilitated intracellular growth.
Abnormal rhythmic motor response in children with attention-deficit–hyperactivity disorder (2003)

Children with attention-deficit-hyperactivity disorder (ADHD) have difficulties with motor control, inhibition of motor responses, motor flexibility, and motor preparedness. We proposed that motor abnormalities in ADHD might result, at least in part, from an abnormal neuronal oscillatory mechanism necessary for motor temporal regulation. The aim of this study was to assess pacing in children with ADHD, by testing for rhythmic abnormalities of motor activity using a tapping test. Twenty-seven children (21 males, six females; aged 6 to 14 years 6 months; mean age 11 years 4 months, SD 2 years 2 months) diagnosed with ADHD according to DSM-IV clinical criteria, and 33 controls (25 males, eight females; aged 6 to 14 years 6 months; mean 11 years 1 month, SD 2 years 2 months), underwent a finger-tapping test requiring rhythmic responses to frequencies from 1 to 6 Hz. All participants who were treated on a daily basis with methylphenidate (n = 22) were medication-free on the day of the test. Most of the children with ADHD responded at a constant rate regardless of stimulus frequency, a phenomenon only seen in a small number of the controls. This specific error pattern, also seen in Parkinson's disease, has been attributed to an abnormal oscillatory mechanism mediated by dopaminergic fronto-striatal circuitry, which might also be pathophysiologically relevant for ADHD.
Resting B cells can act as antigen presenting cells in vivo andinduce antibody responses (1993)

Although it is well established that B lymphocytes are able to present antigen in vitro, the ability of small resting B cells to act as antigen presenting cells in vivo remains controversial. In this report we have studied the antigen presentation and the antibody response induced by mouse B cells after in vivo or in vitro targeting antigens to membrane Ig (mIg), using rat mAbs. Our results show that injection of these mAbs coupled to 2,4-dinitrophenyl (DNP) strongly enhances the IgG1 antibody response against DNP and rat Ig. T cell depleted spleen cells pulsed in vitro with rat Ig without specificity for B cells induced an antibody response when re-injected into mice, this response being much higher if the antigen was specific for mIg. Moreover, purified resting B cells were shown to induce a specific IgG1 response in vivo only when they were cultured with rat mAb against mIgM or mIgD but not with myeloma rat Ig of the same isotype. B cells do not need to be activated to present antigen since the induction of the specific antibody response does not correlate with the mitogenic activity of rat mAb nor with the IgG1 polyclonal synthesis in vivo. These data clearly show that resting B cells can present antigen in vivo and induce an antibody response, and underline the importance of mIgM and mIgD as targets for antigens.
In Vivo Electroporation Using an Exponentially Enhanced Pulse: A New Waveform (2001)

In vivo electroporation is currently accomplished by one of two types of common waveforms: exponential decay or square-wave pulses. The purpose of this report is to present a new electroporation waveform, the exponentially enhanced pulse (EEP). Pulsing protocols including the EEP resulted in high levels of luciferase expression in muscle and skin, equal to or greater than expression resulting from low-voltage, millisecond square-wave pulses. This high level of expression requires fewer pulses when using an EEP protocol. Therefore, similar or greater plasmid DNA expression levels are obtained using fewer pulses with the EEP protocol than with current protocols. This is the first report of this new waveform and shows the success of using protocols employing the EEP to deliver plasmid DNA to various tissue types.
LIQUID FILM THICKNESS MEASUREMENT (2002)

The extensive information available in the literature for liquid film thickness measurement in multiphase systems has been critically reviewed. The review is structured by classification of the identified measurement techniques into groups of comparable measurement resolution. Each technique is reviewed in terms of development history, methodology and technical requirements. Information on the measurement accuracy of each technique and the results of comparative tests between techniques is supplied, where available. The review concludes with the author's thoughts on recommendation of specific techniques dependent on measurement environment and required resolution.
Preparation of calcium phosphate coatings on titanium implant materials by simple chemistry (1998)

A two-step chemical treatment has been developed in our group to prepare commercially pure titanium (cpTi) surfaces that will allow calcium phosphate (Ca-P) precipitation during immersion in a supersaturated calcification solution (SCS) with ion concentrations of [Ca2+] = 3.10 mM and [HPO4(2-)] = 1.86 mM. It was observed that a precalcification (Pre-Ca) procedure prior to immersion could significantly accelerate the Ca-P deposition process. In this work, the bioactivity of chemically treated cpTi and Ti6Al4V was further verified by applying commercially available Hanks' balanced salt solution (HBSS), an SCS with very low ion concentrations of [Ca2+] = 1.26 mM and [HPO4(2-)] = 0.779 mM, as the immersion solution. It was found that a uniform and very dense apatite coating magnesium impurities was formed if the Pre-Ca procedure was performed before immersion, as compared with the loose Ca-P layer obtained from the abovementioned high concentration of SCS. The formation of a microporous titanium dioxide thin surface layer on cpTi or Ti6Al4V by the two-step chemical treatment could be the main reason for the induction of apatite nucleation and growth from HBSS. Variations of pH values, Ca and P concentrations, and immersion time in HBSS were investigated to reveal the detailed process of Ca-P deposition. The described treatments provide a simple chemical method to prepare Ca-P coatings on both cpTi and Ti6Al4V.
Handheld Devices for Control (2018)

With present and future wireless technologies, such as IEEE 802.11, BlueTooth, RF-Lite, and G3, handheld devices will frequently be in close, interactive communication. Many environments, including offices, meeting rooms, automobiles and classrooms already contain many computers and computerized appliances, and the smart homes of the future will have ubiquitous embedded computation. When the user enters one of these environments carrying a handheld device, how will that device interact with the environment? We are exploring, as part of the Pebbles research project, the many ways that handheld devices such as PalmOS Organizers, PocketPC and Windows CE devices, and smart cell phones can serve as useful adjuncts to the “fixed” computers and computerized appliances in the user’s vicinity. This brings up many interesting research questions, such as: How can the handheld device <em>improve</em> the user interfaces of everything else in the user’s environment, rather than being just be another complex gadget that must be learned? What is the best way to provide a user interface that spans multiple devices that are in use at the same time? How will users and systems decide which functions should be presented and in what manner on what device? How can the user’s handheld device be effectively used as a “Personal Universal Controller” to provide an easy-to-use and familiar interface to all of the complex appliances available to a user? How can communicating handheld devices enhance the effectiveness of meetings and classroom lectures? We present some preliminary observations on these issues, and discuss some of the systems that we have built to investigate them.
Synthesis of 2,4‐diaminopyrrolo[2,3‐d]pyrimidines via thermal fischer indolization. Pyrazole formation with ytterbium triflate catalysis (2000)

Abstract The high‐yield synthesis of the 2,4‐diaminopyrrolo[2,3‐ d ] pyrimidine 4 (PNU‐87663) via a Bischler‐like alkylation‐cyclization sequence was reported earlier. We describe herein an alternative synthesis of this potent antioxidant and several analogs based on the thermal Fischer indolization, starting from hydrazino substituted pyrimidines 5 and 13. In several cases where the thermal Fischer indolization failed, attempts to catalyze the reaction with Lewis acids, especially ytterbium triflate, led to the surprising and unprecedented formation of pyrazolo[3,4‐ d ]pyrimidines, e.g. 1‐methyl‐3‐phenyl‐4,6‐di‐1‐pyrrolidinyl‐1 H ‐pyrazolo[3,4‐ d ]pyrimidine (24), with the loss of the elements of methane. Mechanistic details of this transformation remain to be investigated.
Excess Factor VIII: A Common Cause of Hypercoagulability (2005)

Factor VIII assay should be considered in the work-up of idiopathic, recurrent VTE. Long-term anticoagulation may be appropriate in this setting.
Mapping of the gene coding for Epstein-Barr virus-determined nuclear antigen EBNA3 and its transient overexpression in a human cell line by using an adenovirus expression vector (1987)

The open reading frame which lies within the Epstein-Barr virus (EBV) T2 cDNA isolated by Bodescot et al. (M. Bodescot, O. Brison, and M. Perricaudet, Nucleic Acids Res. 14:2611-2620, 1986) was inserted into a eucaryotic expression vector containing a strong adenovirus promoter. The T2 cDNA contains viral genomic sequences from the short BLRF3 open reading frame fused to the adjacent BERF1 long open reading frame. After transfection of human cells, the recombinant plasmid directed the expression of a 140-kilodalton protein. The expressed protein had the same molecular weight, subcellular localization, and immunological characteristics as the EBV-determined nuclear antigen EBNA3, which is made in lymphocytes latently infected with EBV. Immunoprecipitation of extracts of transfected cells labeled with [32P]phosphoric acid showed that the EBNA3 protein is phosphorylated.
Differentiation Therapy of Acute Promyelocytic Leukemia with Tretinoin (All-trans-Retinoic Acid) (1991)

Tretinoin is a safe and highly effective agent for inducing complete remission in patients with acute promyelocytic leukemia. Clinical response to this agent is associated with leukemic-cell differentiation and is linked to the expression of an aberrant RAR-alpha nuclear receptor. Molecular detection of the aberrant receptor may serve as a useful marker for residual leukemia in patients with this disease.
Heat transfer handbook (2003)

Preface. Contributors. 1. Basic Concepts (Allan D. Kraus). 2. Thermophysical Properties of Fluids and Materials (R. T Jacobsen, E. W. Lemmon, S. G. Penoncello, Z. Shan, and N. T. Wright). 3. Conduction Heat Transfer (A. Aziz). 4. Thermal Spreading and Contact Resistances (M. M. Yovanovich and E. E. Marotta). 5. Forced Convection: Internal Flows (Adrian Bejan). 6. Forced Convection: External Flows (Yogendra Joshi and Wataru Nakayama). 7. Natural Convection (Yogesh Jaluria). 8. Thermal Radiation (Michael F. Modest). 9. Boiling (John R. Thome). 10. Condensation (M. A. Kedzierski, J. C. Chato, and T. J. Rabas). 11. Heat Exchangers (Allan D. Kraus). 12. Experimental Methods (Jose L. Lage). 13. Heat Transfer in Electronic Equipment (Avram Bar-Cohen, Abhay A. Watwe, and Ravi S. Prasher). 14. Heat Transfer Enhancement (R. M. Manglik). 15. Porous Media (Adrian Bejan). 16. Heat Pipes (Jay M. Ochterbeck). 17. Heat Transfer in Manufacturing and Materials Processing (Richard N. Smith, C. Haris Doumanidis, and Ranga Pitchumani). 18. Microscale Heat Transfer (Andrew N. Smith and Pamela M. Norris). 19. Direct Contact Heat Transfer (Robert F. Boehm). Author Index. Subject Index. About the CD-ROM.
Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation (2013)

Integration is essential for HIV-1 replication, and the viral integrase (IN) protein is an important therapeutic target. Allosteric IN inhibitors (ALLINIs) that engage the IN dimer interface at the binding site for the host protein lens epithelium-derived growth factor (LEDGF)/transcriptional coactivator p75 are an emerging class of small molecule antagonists. Consistent with the inhibition of a multivalent drug target, ALLINIs display steep antiviral dose-response curves ex vivo. ALLINIs multimerize IN protein and concordantly block its assembly with viral DNA in vitro, indicating that the disruption of two integration-associated functions, IN catalysis and the IN-LEDGF/p75 interaction, determines the multimode mechanism of ALLINI action. We now demonstrate that ALLINI potency is unexpectedly accounted for during the late phase of HIV-1 replication. The compounds promote virion IN multimerization and, reminiscent of class II IN mutations, block the formation of the electron-dense viral core and inhibit reverse transcription and integration in subsequently infected target cells. Mature virions are recalcitrant to ALLINI treatment, and compound potency during virus production is independent of the level of LEDGF/p75 expression. We conclude that cooperative multimerization of IN by ALLINIs together with the inability for LEDGF/p75 to effectively engage the virus during its egress from cells underscores the multimodal mechanism of ALLINI action. Our results highlight the versatile nature of allosteric inhibitors to primarily inhibit viral replication at a step that is distinct from the catalytic requirement for the target enzyme. The vulnerability of IN to small molecules during the late phase of HIV-1 replication unveils a pharmacological Achilles' heel for exploitation in clinical ALLINI development.
Protecting Groups in Oligonucleotide Synthesis (1994)

A biopolymer is synthesized by assembling monomeric or oligomeric blocks. Each block features at least a nucleophilic and an electrophilic function, i.e., the α-amino and the carboxylic functions for peptides, the 5′-OH and the 3′-function (phosphate, phosphoramidite, or phosphonate), for nucleotides. The nucleophilic and electrophilic sites are linked together at the coupling step. Protection is a necessity. It guarantees the chemoselectivity of coupling and the solubility of synthons in organic solvents.
Ion stability of nucleic acids in infrared matrix-assisted laser desorption/ionization mass spectrometry (1993)

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) with infrared laser light of a wavelength of 2.94 microns has been used for the analysis of nucleic acids. Spectra of oligodeoxynucleotides up to 26 nucleotides, oligothymidylic acids up to 100 nucleotides as well as different synthetic RNA oligomers and RNA transcripts up to 104 nucleotides are presented. A main problem in the analysis of oligodeoxynucleotides was found to be related to the loss of bases. The stability of oligothymidylic acids as opposed to oligodeoxynucleotides containing all four bases indicates that the loss of bases is correlated with A, C and G protonation which decreases the stability of the N-glycosidic bond. Experiments indicate that the breakage of the N-glycosidic bond probably occurs during the desorption process due to proton transfer from the phosphodiester groups to the ionizable bases. RNA displayed a significantly higher stability in MALDI-MS due to the presence of a 2'-OH group. Consequently, signals of RNA transcripts with a length of up to 142 nucleotides could be detected by MALDI-MS. Technical details of the method, including the distribution of positive counterions on the phosphodiester backbone, the upper mass limit and mass accuracy are discussed along with a number of potential analytical applications.
3D Printing Macroscale Engineered Materials Using Ultrasound Directed Self‐Assembly and Stereolithography (2017)

A manufacturing process to 3D print engineered materials comprised of a user‐specified pattern of nano‐ or microparticles embedded in a polymer matrix material is described. The materials are printed layer‐by‐layer using stereolithography, and in each layer, ultrasound directed self‐assembly is employed to organize a user‐specified pattern of particles. This process allows manufacturing macroscale 3D materials with a user‐specified microstructure consisting of particles of any material, and contrasts with existing processes, which are often limited to laboratory scale, specific materials, and/or 2D implementations. Using this manufacturing process, 3D printing of macroscale multilayer engineered materials containing a Bouligand microstructure commonly found in composite laminate and biological materials is demonstrated. Additionally, engineered materials containing a pattern of electrically conductive nickel‐coated carbon fibers are fabricated, which illustrate the feasibility of 3D printing structures with embedded insulated electrical wiring. This process finds application in manufacturing of multifunctional composite materials.
Chemokines in vascular remodeling (2007)

The arterial vessel wall response to a variety of injuries consists in structural changes, which can result in luminal narrowing and aggravation of the underlying disease. This arterial remodeling is characterized by neointima formation and medial thickening, inflammatory cell recruitment and endothelial dysfunction. Chemokines and the corresponding receptors have been shown to participate at every step of the remodeling process. The monocyte chemotactic protein (MCP)-1/CC motif receptor 2 (CCR2) axis induces monocyte infiltration of the injured vessel wall and can stimulate proliferation of smooth muscle cells (SMCs) in models of restenosis, cardiac allograft vasculopathy (CAV), pulmonary hypertension, and systemic hypertension. In contrast, stromal cell-derived factor (SDF)-1 alpha and its receptor CXC motif receptor 4 (CXCR4) are centrally involved in the neointimal recruitment of SMC progenitor cells (SPCs), presumably in response to SMC apoptosis, in restenosis and CAV. The RANTES (Regulated upon activation, normally T-cell expressed, and presumably secreted) receptors CC motif receptor 1 (CCR1) and CC motif receptor 5 (CCR5) affect intimal monocyte infiltration and neointimal growth, which could be due to the deposition of platelet-derived RANTES on activated endothelial cells. Fractalkine is expressed on neointimal SMCs and thus mediates the arrest of monocytes. Interestingly, reendothelialization of injured vessels appears to primarily depend on CXC motif ligand 1 (CXCL1). These chemokine effects form a complex network, which operates in all mechanisms of vascular remodeling. The detailed understanding of the function of the chemokine network in the remodeling process may allow specific disease intervention.