gebhart/patrepeval huggingface dataset:
Photoreceptors of mouse retinas possess D4 receptors coupled to adenylate cyclase. (1992)

In the mouse, the light-sensitive pool of cAMP can be eliminated in the dark by application of the dopamine D2-like receptor agonists LY 171555 (quinpirole), (+)-N0437 (2-[N-(n-propyl)-N-2-(thienylethylamino)-5-hydroxytetralin]) , or (+)-3-PPP [3-(3-hydroxyphenyl)-N-propylpiperidine hydrochloride]. The rank-order affinity of the ability of the D2-like antagonists to block the action of LY 171555 matched that of the rat D4 receptor. Reverse transcription of retina mRNA followed by DNA amplification using D4-specific nucleotides demonstrates the presence of D4 mRNA in retina. In situ hybridization studies using D4-specific digoxygenin-labeled oligonucleotides or 35S-labeled UTP RNA probes demonstrate the presence of D4 mRNA in the photoreceptor cell layer and in the inner nuclear and ganglion cell layers. The modulation by D4 ligands of the dark level of light-sensitive cAMP in photoreceptors demonstrates the physiological coupling of the D4 receptor subtype.
Transcriptional Analysis of Tumor-Specific T-Cell Responses in Cancer Patients (2002)

Over the last decade, tumor immunology in general and tumor immunotherapy in particular have made important progress. Thanks to the discovery of tumor-associated antigens (TAA), and the consequent development of active-specific immunization protocols, TAA-specific T-cell responses can now be induced reproducibly in cancer patients. However, clinical responses directly ascribable to TAA-specific T cells occur only occasionally. It is not clear why TAA-specific T cells do not eliminate tumor cells in vivo. This paradoxical coexistence of antigen-bearing tumor cells and antigen-specific T cells constitutes a critical point of current investigation. In recent years, protein-ligand interaction-based methods aimed at the identification and characterization of T cells using antibodies (cell surface phenotyping, intracellular and secreted cytokine detection), or HLA-peptide multimers, have significantly improved the analysis of TAA-specific T cells. These methods, however, seem to have reached the limit of their usefulness. Transcriptional analysis may add sensitivity and resolution and may provide global pictures of the multifactorial requirements for an efficient immune response against tumors. In this review, we describe the use of molecular genetic methods, such as real time qRT-PCR, cDNA microarrays, and TCR repertoire analysis. In addition, we describe techniques for high-fidelity messenger RNA amplification that allow high-throughput analysis of samples obtained from minimal sources, such as HLA/peptide tetramer sorted antigen-specific T cells, laser capture dissection, or fine needle aspirates. Recent work discussed in this review summarizes the complementarity of transcriptional analysis as an essential tool that, in addition to conventional methods, may deepen and broaden the characterization of tumor-specific T cells.
Ecopolitics: building a green society (1994)

Where do you place the blame for the environmental crisis - too many people? consumer greed? technology gone amok? And what do you think will save our planet - birth control? appropriate technology? recycling? eco-consumerism? Those solutions are just Band-Aids on a bleeding Earth, argues environmental activist Daniel A. Coleman. Where conventional wisdom sees both the cause of the environmental crisis and its cure in individual actions, Coleman says: Look again. By blaming ourselves as individuals, we let governments and corporations off the hook. Making 50 simple changes in our personal lifestyles is worthwhile, but must not divert our attention from the underlying causes of environmental disaster. The real causes are rooted deep in the politics of human affairs - and so are their solutions. We should be asking: Why do we allow such harm to our environment? How did we create a society with no stake in the future? How can we build a green society? The good news is that we can reverse the process of environmental abuse. Political strategies driven by the key values of ecological responsibility, participatory democracy, environmental justice, and community action are effective. Dan Coleman's stories of citizen groups whose grassroots organizing has already put ecologically sound policies in place demonstrate that the sustainable society is indeed possible. Lucid, lively, probing, serious, yet optimistic - Coleman's analysis is required reading for all who count the earth as their home.
A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer region in Cre-mediated recombination (2006)

This study approached loxP spacer mutant screening in an unbiased manner, assuming nothing about candidate loxP sites save for the conserved 4 and 5 spacer positions. Candidate sites were free to recombine with any other sequence in the pool of all possible sites. The subset of loxP sites identified here are candidates for in vivo serial recombination as they have already demonstrated limited promiscuity with other loxP spacer and stability in the presence of Cre.
Genomic characterization of CD84 reveals the existence of five isoforms differing in their cytoplasmic domains (2000)

CD84, a new member of the immunoglobulin superfamily, shows high homology with several molecules belonging to the CD2 family of differentiation antigens. By screening a peripheral blood leukocyte cDNA library four CD84 isoforms were obtained differing in their 3' sequence. A reverse transcription-polymerase chain reaction (RT-PCR) analysis confirmed that these isoforms were normally found on leukocytes and a new isoform was identified. To establish the nature of the five isoforms obtained (CD84a, CD84b, CD84c, CD84d and CD84e) the genomic structure of the CD84 gene was determined. Our results show that it is composed of at least eight exons, with an exon coding for the 5' UTR and the leader peptide, two exons coding for each of the two immunoglobulin-like domains, an exon encoding the transmembrane portion and four exons coding for the cytoplasmic domains. The isoforms are generated by several mechanisms: alternative use of exons, reading frame shift, use of a cryptic splice site or absence of splicing. The differential expression of several potentially phosphorylatable residues on the different isoforms could be a way to regulate its possible activity in signal transduction.
Binding of novel macrolide structures to macrolides-lincosamides-streptogramin B-resistant ribosomes inhibits protein synthesis and bacterial growth (1989)

Dimethylation of adenine 2058 in 23S rRNA renders bacteria resistant to macrolides, lincosamides, and streptogramin B (MLS resistance), because the antibiotic binding site on the altered 50S ribosomal subunit is no longer accessible. We now report that certain 6-O-methyl-11,12-cyclic carbamate derivatives of erythromycin are able to bind to dimethylated MLS-resistant 50S ribosomal subunits, thus inhibiting protein synthesis and cell growth. One of these novel structures, an 11-deoxy-11-(carboxyamino)-6-O-methylerythromycin A 11,12-(cyclic ester) derivative, structure 1a, was studied in detail. It inhibited in vitro protein synthesis in extracts prepared from both susceptible and MLS-resistant Bacillus subtilis with 50% inhibitory concentrations of 0.4 and 20 microM, respectively. The derivative bound specifically to a single site on the 50S subunit of MLS-resistant ribosomes prepared from B. subtilis and Staphylococcus aureus, and no binding to 30S subunits was observed. The association rate constant of derivative 1a with sensitive and resistant ribosomes was 100- and 500-fold slower, respectively, than that of the parent compound, erythromycin, with sensitive ribosomes. The dissociation rate constant of 1a from sensitive and resistant ribosomes was 50- to 100-fold slower than the rate of erythromycin dissociation from sensitive ribosomes. Furthermore, 1a binding to sensitive 50S subunits led to induction of ermC and ermD, while binding to resistant 50S subunits did not, showing that perturbation of sensitive and resistant 50S subunit function by 1a differs. These data demonstrated that 1a is unique in its interaction with MLS-resistant ribosomes and that this interaction causes a novel allosteric perturbation of ribosome function.
MODELING CONFIGURABLE PRODUCT FAMILIES (1998)

Configurable products offer a way to efficiently manage product families with large numbers of variants. Some one-of-a-kind products can also be transformed to configurable products. Product configuration requires both systematic processes and appropriate modeling. We outline a method for modeling a product family as a configurable product. The method is
The Pulmonary Response to Sublethal Thoracic Irradiation in the Rat (1993)

An animal model of radiation-induced lung disease was established using male Wistar rats given sublethal bilateral thoracic irradiation (15 Gy). The rats were studied for up to 20 weeks and compared to sham-irradiated controls. Three distinct syndromes were identified. Two weeks after irradiation there was an increase in wet lung weight without an increase in dry lung weight. Interstitial edema was confirmed ultrastructurally, but aside from minor abnormalities of endothelial cells, both capillary and alveolar basement membranes were intact and there was no alveolar protein leak. At 4 weeks after irradiation, there was an abrupt increase in both wet and dry lung weights, as well as intra-alveolar macrophages, lymphocytes, polymorphs, and protein. These changes persisted for periods of up to 8 weeks. Electron microscopy at 4 weeks revealed prominent interstitial edema and severe endothelial cell damage. There was patchy thickening of the cytoplasm of type I cells as well as some cells which appeared to be transforming from type II to type I cells, suggesting previous epithelial denudation. Mast cell density increased in perivascular and peribronchial areas from 4 weeks, and this and parenchymal mast cell density peaked at 7 weeks. The total collagen content of the lungs (determined biochemically) rose by up to 50% above control values from 5 weeks after irradiation, the bulk of the increase having occurred by 12 weeks. Further increases up to 20 weeks were similar to that seen in growing control animals. Collagen deposition (as defined by electron microscopy and Picrosirius polarization) was prominent in peribronchial and perivascular areas in all animals, but in alveolar walls it was increased severalfold above controls by 20 weeks after irradiation. In summary, this model provides sequential changes of interstitial edema, alveolitis, and interstitial fibrosis which can be studied independently. The temporal relationship between the appearance of mast cells and increased collagen deposition supports the hypothesis that mast cells are intimately related to the development of fibrosis.
Comparision of laser-induced and classical ultasound (2003)

A classical medical ultrasound system was combined with a pulsed laser source to allow laser-induced ultrasound imaging (optoacoustics). Classical ultrasound is based on reflection and scattering of an incident acoustic pulse at internal tissue structures. Laser-induced ultrasound is generated in situ by heating optical absorbing structures, such as blood vessels, with a 5 ns laser pulse (few degrees or fraction of degree), which generates pressure transients. Laser-induced ultrasound probes optical properties and therefore provides much higher contrast and complementary information compared to classical ultrasound. An ultrasound array transducer in combination with a commercial medical imaging system was used to record acoustic transients of both methods. Veins and arteries in a human forearm were identified <i>in vivo</i> using classical color doppler and oxygenation dependent optical absorption at 660 nm and 1064 nm laser wavelength. Safety limits of both methods were explored. Laser-induced ultrasound seems well suited to improve classical ultrasound imaging of subcutaneous regions.
Reactivation of Chlamydia trachomatis lung infection in mice by cortisone (1983)

To study the latency, chronicity, and recurrent nature of chlamydial infection, we attempted to reactivate Chlamydia trachomatis lung infection in mice by immunosuppressive therapy with cortisone. Mice were treated with subcutaneous injections of cortisone acetate (125 mg/kg) every other day, starting on day 14 after intranasal inoculation of C. trachomatis serotype B (TW-5). C. trachomatis was recovered from the lungs beginning day 6 after the start of cortisone treatment until the end of the observation period on day 12 of treatment. Overall, the reactivation was successful in 8 of 55 mice treated with cortisone, in contrast to 0 of 41 inoculated, untreated mice (P = 0.009) and 0 of 35 uninoculated, treated mice. Cortisone treatment affected the ability of peritoneal exudate cells to respond to migratory inhibition after exposure to purified whole organisms of C. trachomatis serotype B (TW-5) but had little effect on serum antibody titers, indicating a possible role for cellular immunity in resistance against C. trachomatis infection in the lung.
Reconstructing Relief Surfaces (2004)

This paper generalizes Markov Random Field (MRF) stereo methods to the generation of surface relief (height) fields rather than disparity or depth maps. This generalization enables the reconstruction of complete object models using the same algorithms that have been previously used to compute depth maps in binocular stereo. In contrast to traditional dense stereo where the parametrization is image based, here we advocate a parametrization by a height field over any base surface. In practice, the base surface is a coarse approximation to the true geometry, e.g., a bounding box, visual hull or triangulation of sparse correspondences, and is assigned or computed using other means. A dense set of sample points is defined on the base surface, each with a fixed normal direction and unknown height value. The estimation of heights for the sample points is achieved by a belief propagation technique. Our method provides a viewpoint independent smoothness constraint, a more compact parametrization and explicit handling of occlusions. We present experimental results on real scenes as well as a quantitative evaluation on an artificial scene.
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) fromMycobacterium tuberculosis (2005)

Diaminopimelate decarboxylase from Mycobacterium tuberculosis (LysA, DAPDC, Rv1293) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized. Preliminary diffraction data analysis suggests the presence of a homotetramer in the asymmetric unit.
노인체육 편 : 노인들의 생활체육 참여가 여가만족도와 정신건강과의 관계 (2001)

This research focuses on satisfaction of old man which participation of sports or not. Next describes result of the research. 1) Extent of satisfaction rate of subjects for leisure satisfaction bases on general characteristics. A. There is difference between participants and non-participant for physical, psychological, educational, environmental fields. Especially participant group shows much higher satisfaction rate for all the fields than that of non-participant group. B. Age shows difference among physical, psychological, educational, environmental fields. Especially, Rate of all the leisure satisfaction under 70yrs is much higher than that of over 79yrs. C. Career shows difference in psychological, social, educational, environmental field, Especially the group who has independent business career shows much higher satisfaction rate for all the field than that of housewives group. 2) Extent of satisfaction rate of subjects for psychological health bases on general characteristics. A. House owner group shows much lower psychological unstability rate than that of non-house owner group. B. Career shows difference. Housewives group shows much higher psychological unstability rate than general group. C. Personal expense shows difference. Less than 100,000won expense group shows much higher ps than more than 210,000won expense group. 3) Extent of satisfaction rate of subjects for psychological health bases on leisure satisfaction rate. Physical, psychological, social, educational, environmental leisure satisfaction rate causes negative affection to psychological health. In other words, high physical satisfaction rate means low psychological unstability and high psychological satisfaction rate means low psychological unstability.
Adult glioma incidence trends in the United States, 1977–2000 (2004)

The results in the current study were consistent with other published reports that showed an increase in the incidence of brain tumors using SEER data. Although others have observed increasing incidence trends among the elderly, the authors formally tested and found a statistically significant interaction between age at diagnosis and year of diagnosis.
Point mutation impairs centromeric CENH3 loading and induces haploid plants (2015)

The chromosomal position of the centromere-specific histone H3 variant CENH3 (also called "CENP-A") is the assembly site for the kinetochore complex of active centromeres. Any error in transcription, translation, modification, or incorporation can affect the ability to assemble intact CENH3 chromatin and can cause centromere inactivation [Allshire RC, Karpen GH (2008) Nat Rev Genet 9 (12):923-937]. Here we show that a single-point amino acid exchange in the centromere-targeting domain of CENH3 leads to reduced centromere loading of CENH3 in barley, sugar beet, and Arabidopsis thaliana. Haploids were obtained after cenh3 L130F-complemented cenh3-null mutant plants were crossed with wild-type A. thaliana. In contrast, in a noncompeting situation (i.e., centromeres possessing only mutated or only wild-type CENH3), no uniparental chromosome elimination occurs during early embryogenesis. The high degree of evolutionary conservation of the identified mutation site offers promising opportunities for application in a wide range of crop species in which haploid technology is of interest.
An Intelligent Home Energy Management System to Improve Demand Response (2013)

Demand Response (DR) and Time-of-Use (TOU) pricing refer to programs which offer incentives to customers who curtail their energy use during times of peak demand. In this paper, we propose an integrated solution to predict and re-engineer the electricity demand (e.g., peak load reduction and shift) in a locality at a given day/time. The system presented in this paper expands DR to residential loads by dynamically scheduling and controlling appliances in each dwelling unit. A decision-support system is developed to forecast electricity demand in the home and enable the user to save energy by recommending optimal run time schedules for appliances, given user constraints and TOU pricing from the utility company. The schedule is communicated to the smart appliances over a self-organizing home energy network and executed by the appliance control interfaces developed in this study. A predictor is developed to predict, based on the user's life style and other social/environmental factors, the potential schedules for appliance run times. An aggregator is used to accumulate predicted demand from residential customers.
Induction of IgG antibodies against the GD2 carbohydrate tumor antigen by vaccination with peptide mimotopes (2006)

The disialoganglioside GD2, a carbohydrate antigen, is expressed on all tumors of neuroectodermal origin, including melanoma, neuroblastoma, sarcoma and small cell lung cancer. Due to its specific expression on tumor surfaces, GD2 is an attractive target for immunotherapies. The mouse/human chimeric anti-GD2 mAb ch14.18 is already applied in melanoma and neuroblastoma trials as a passive immunotherapy. To establish an active immunotherapy alternative, we aimed to replace the poorly immunogenic ganglioside with immunogenic peptides. Previously, we used the ch14.18 antibody to select GD2 peptide mimics from a phage display library. In the present study, two mimics of the ch14.18 epitope were coupled to keyhole limpet hemocyanin and used for immunizing BALB/c mice. Induction of a specific humoral immune response towards the original antigen GD2, both purified and expressed on neuroblastoma and melanoma cells, could be demonstrated in ELISA, Western blot, and immunofluorohistochemistry. As the elicited antibodies were of the IgG isotype, the mimotope conjugates were capable of recruiting T cell help and inducing memory phenomena. In conclusion, we show that an epitope of the carbohydrate antigen GD2 can successfully be translated into immunogenic peptide mimotopes. Our immunization experiments indicate that GD2 mimotopes are suitable for active immunotherapy of GD2-expressing tumors.
Synthesis and Pharmacological Investigation of New Arylethanolamines as β3-Adrenoceptor Ligands (1999)

A series of arylethanolamines with the amino group bearing a variety of bulky alicyclic residues was synthesized.



The para position of the benzene ring was variously substituted to further vary lipophilic and steric properties. The meta-chloro substituted derivatives were also considered. Thirteen representative compounds were tested for their ability to displace [3H]CGP 12177 from rat recombinant β3-adrenoceptors and some were found to exhibit activity comparable with that of BRL 37344, chosen as the reference drug.



N-Alicyclic substituents can profitably replace the commonly used N-arylalkyl substituents in this class of drugs.
Comparisons of ELISA and Western blot assays for detection of Cryptosporidium antibody (1998)

A seroprevalence survey was conducted using ELISA and Western blot (WB) assays for antibody to three Cryptosporidium antigens on 380 blood donors in Jackson County, Oregon. The purpose was to determine if either assay could detect serological evidence of an outbreak which occurred in Talent, Oregon 6 months earlier. The ELISA, which tested for combined IgG, IgA and IgM, and the WB, which tested separately for IgG and IgA, detected an almost twofold increase in serological response for persons who consumed Talent drinking water during the previous 11 months. The increases, however, were statistically significant (P < 0.05) only for the WB. The identification of serological evidence of infection, using sera collected 6 months after the end of the outbreak in a population not selected because of cryptosporidiosis-like illness, suggests that assays of Cryptosporidium-specific IgG and IgA may assist in estimating the magnitude of asymptomatic infections in the population.