gebhart/patrepeval huggingface dataset:
Trehalose-Based Eye Drops Preserve Viability and Functionality of Cultured Human Corneal Epithelial Cells during Desiccation (2014)

This paper presents the evaluation of cytoprotective ability of trehalose-based eye drops in comparison with commercially available preparations during the experimental desiccation of cultured human corneal epithelial cells. Cultured human corneal epithelial cells (hCEC) underwent incubation with 7 different, commercially available medicaments used commonly in dry eye syndrome treatment, followed by desiccation trial performed on air under the flow hood for 5, 15, 30, and 45 minutes. Cell viability was quantified by live/dead fluorescent assay, while the presence of apoptotic cells was estimated by immunofluorescent staining for active caspase 3 protein. The preservation of membrane functions was evaluated using neutral red staining, while the preservation of proper morphology and phenotype was determined by fluorescent staining for actin filaments, nuclei, and p63 protein. The trehalose-based eye drops showed the highest efficiency in prevention of cell death from desiccation; moreover, this preparation preserved the normal cellular morphology, functions of cell membrane, and proliferative activity more effectively than other tested medicaments.
Construction and Structural Adhesives and Sealants: An Industrial Guide (1988)

This work describes more than 1,600 construction and structural adhesives and sealants currently available from over 100 suppliers. These adhesives and sealants can be used for major construction and structural applications as well as in commercial businesses, schools, and offices, and by the home-repair handyman. The products can be applied to such diverse materials as concrete, ceramics, vinyl, polyethylene, foamed plastics, wood, plywood, fiberboard, gypsum board, prefinished panels, leather, and cotton.
The TPR-MET oncogenic rearrangement is present and expressed in human gastric carcinoma and precursor lesions. (1991)

The TPR-MET oncogenic rearrangement was originally observed in an in vitro transformed human osteosarcoma cell line. Recently, we detected the expression of this rearrangement at very low levels in several cell lines derived from human tumors of nonhematopoietic origin using a highly sensitive method based on polymerase chain reaction amplification of the transcript. We report here the results of analysis of TPR-MET expression in cell lines derived from human gastric tumors and 22 biopsy samples of human gastric mucosa showing cancer or precursor lesions. The rearranged RNA was expressed in all four cell lines as well as in biopsy samples from 12 of the 22 patients. Overexpression of TPR-MET RNA in superficial gastritis lesions with hyperplasia of glandular neck cells suggests the possible involvement of this oncogene at an early stage of gastric tumorigenesis. Analysis of gastric biopsy samples for RAS gene mutations showed base substitutions occurring in the codon 12 region of Ki- and Ha-RAS genes in four cases, including two precursor lesions.
Modulation of the folate receptor type β gene by coordinate actions of retinoic acid receptors at activator Sp1/ets and repressor AP-1 sites (2003)

Folate receptor (FR) type beta is a promising target for therapeutic intervention in acute myelogenous leukemia (AML) owing particularly to its specific up-regulation in AML cells by all-trans retinoic acid (ATRA). Here we identify functional elements in the FR-beta gene and examine the molecular mechanism of transcriptional induction of FR-beta by ATRA. The basal promoter activity of FR-beta resulted from synergistic interaction between Sp1 and ets binding sites (EBSs) and repression by upstream AP-1-like elements, whose action required EBSs. A minimal promoter containing the Sp1 and ets elements was ATRA-responsive. The repressor elements bound Fos family proteins; association of the proteins with the repressor elements correlated negatively with FR-beta expression in peripheral blood neutrophils and monocytes and also in KG-1 (AML) cells grown in the absence or in the presence of ATRA. Furthermore, down-regulation of FR-beta in KG-1 cells treated with O-tetradecanoylphorbol 13-acetate (TPA) was accompanied by increased AP-1 binding to the repressor elements. From chromatin immunoprecipitation (ChIP) assays, the nuclear retinoic acid receptor alpha (RARalpha) associated with the Sp1 region, and RARs beta and gamma associated with the AP-1 and Sp1 regions; treatment of KG-1 cells with ATRA did not alter Sp1 binding but increased the association of RARalpha and decreased the association of RARs beta and gamma. ATRA also decreased RAR expression levels. The results suggest that the FR-beta gene is a target for multiple coordinate actions of nuclear receptors for ATRA directly and indirectly acting on a transcriptional complex containing activating Sp1/ets and inhibitory AP-1 proteins. The multiple mechanisms favor the prediction that ATRA will induce FR-beta expression in a broad spectrum of AML cells. Further, optimal FR-beta induction may be expected when all 3 RAR subtypes bind agonist.
Functional regulatory immune responses against human cartilage glycoprotein-39 in health vs. proinflammatory responses in rheumatoid arthritis (2004)

The class of immune response against autoantigens could profoundly influence the onset and/or outcome of autoimmune diseases. Until now, there is only limited information on the antigen-specific balance between proinflammatory and regulatory responses in humans. Here we analyzed the natural immune response against a candidate autoantigen in rheumatoid arthritis, human cartilage glycoprotein-39 (HC gp-39). Peripheral blood mononuclear cells from healthy individuals reacted against HC gp-39 with the production of IL-10 but not IFN-gamma. Ex vivo assays indicated that the naturally occurring HC gp-39-specific immune response in bulk is powerful enough to suppress antigen-specific recall responses, demonstrating that rather than being unresponsive, the HC gp-39-directed immune response in healthy individuals shows a strong bias toward a regulatory phenotype. Moreover, CD4(+) T cell lines directed against HC gp-39 expressed CD25, glucocorticoid-induced tumor necrosis factor receptor, and Foxp3 molecules and were capable of suppressing antigen-specific T cell responses. Cell-cell contact was required for this suppression. As opposed to healthy individuals, the HC gp-39-directed immune response in 50% of patients with rheumatoid arthritis exhibits polarization toward a proinflammatory T helper 1 phenotype and is significantly less powerful in suppressing antigen-specific recall responses. Together these findings indicate that the presence of HC gp-39-specific immune responses in healthy individuals may have an inhibitory effect on inflammatory responses in areas where HC gp-39 is present. Furthermore, these data indicate that the class of HC gp-39-directed immune response in rheumatoid arthritis patients has shifted from an antiinflammatory toward a proinflammatory phenotype.
Prediction of functional residues in water channels and related proteins (1998)

In this paper, we present an updated classification of the ubiquitous MIP (Major Intrinsic Protein) family proteins, including 153 fully or partially sequenced members available in public databases. Presently, about 30 of these proteins have been functionally characterized, exhibiting essentially two distinct types of channel properties: (1) specific water transport by the aquaporins, and (2) small neutral solutes transport, such as glycerol by the glycerol facilitators. Sequence alignments were used to predict amino acids and motifs discriminant in channel specificity. The protein sequences were also analyzed using statistical tools (comparisons of means and correspondence analysis). Five key positions were clearly identified where the residues are specific for each functional subgroup and exhibit high dissimilar physico-chemical properties. Moreover, we have found that the putative channels for small neutral solutes clearly differ from the aquaporins by the amino acid content and the length of predicted loop regions, suggesting a substrate filter function for these loops. From these results, we propose a signature pattern for water transport.
Nutrition and wound healing (1990)

Assistant Professor and Coordinator of Adult Health Nursing San Diego State University School of Nursing Associate Professor and Chair Critical Care Nurse Specialist Graduate Program San Diego State University School of Nursing San Diego, California
Diagnosis of tissue pathology with elastic scattering spectroscopy (2002)

An optical biopsy system based on elastic-scatter spectroscopy has been developed. This system uses white light excitation, is relatively inexpensive, endoscopically compatible, and portable. Light is delivered to the tissue by one or more optical fibers. The OBS has been tested on bladder cancer with excellent results. Preliminary data on the usefulness of the OBS to diagnose dysplasia in the esophagus and detect adenomatous polyps indicate that the OBS may also be useful in these arenas.
Light is required for conidiation in Aspergillus nidulans. (1990)

Light is necessary for asexual sporulation in Aspergillus nidulans but will elicit conidiation only if irradiation occurs during a critical period of development. We show that conidiation is induced by red light and suppressed by an immediate shift to far red light. Conidiation-specific gene functions switch from light-independent to light-dependent activities coincident with the expression of brlA, a regulator of conidiophore development. We also show that light dependence is abolished by a mutation in the velvet gene, which allows conidiation to occur in the absence of light. We propose that the initiation of late gene expression is regulated by velvet and controlled by a red light photoreceptor, whose properties are reminiscent of phytochrome-mediated responses observed in higher plants.
GaInAsP/InP SBH surface emitting laser with Si/Al 2 O 3 mirror (1995)

An Si/Al2O3 mirror has been introduced into the p-side mirror of a 1.3 µm GaInAsP/InP square buried heterostructure (SBH) surface emitting (SE) laser to decrease thermal resistance. The low threshold pulsed oscillation (threshold current Ith = 12 mA) has been obtained for an 8 × 8 µm device at room temperature. Its threshold current density was ~18 kA/cm2. Near room temperature (13°C) CW oscillation for another chip has also been realised.
Blocking interleukin-1β in acute and chronic autoinflammatory diseases (2010)

An expanding spectrum of acute and chronic inflammatory diseases is considered 'autoinflammatory' diseases. This review considers autoinflammatory diseases as being distinct from 'autoimmune' diseases. Autoimmune diseases are associated with dysfunctional T cells and treated with 'biologicals', including antitumour necrosis factorα, CTLA-Ig, anti-IL-12/23, anti-CD20, anti-IL-17 and anti-IL-6 receptor. In contrast, autoinflammatory diseases are uniquely attributed to a dysfunctional monocyte caspase 1 activity and secretion of IL-1β; indeed, blocking IL-1β results in a rapid and sustained reduction in the severity of most autoinflammatory diseases. Flares of gout, type 2 diabetes, heart failure and smouldering multiple myeloma are examples of seemingly unrelated diseases, which are uniquely responsive to IL 1β neutralization.
Endocrine Pharmacology of Antiestrogens as Antitumor Agents* (1990)

IN 1958, Lerner and co-workers (1) described the biological properties of the first nonsteroidal antiestrogen MER25 (see Fig. 1). The discovery of this new class of drugs opened up a variety of clinical possibilities (2, 3), but early studies were terminated because of toxic side effects. Another member of this new class of drugs, clomiphene (then known as chloramiphene or MRL41), held the promise of being a potential antifertility agent (4); however, clinical studies demonstrated that ovulation was induced (5). The drug is now available as a profertility agent in subfertile women (6). During the 1960s a range of antiestrogens was synthesized by the pharmaceutical industry for potential applications in gynecology (7, 8). In the main though, these agents were used only to study reproductive endocrinology in the laboratory. However the focus of clinical application slowly changed from the broad market of contraception to the relatively small market of advanced breast cancer therapy.
Dimethyl fumarate in multiple sclerosis: latest developments, evidence and place in therapy (2016)

Dimethyl fumarate (DMF) is one of the newer additions to the armamentarium of potent immunomodulators for the treatment of relapsing-remitting multiple sclerosis (RRMS). After more than 2 years of real-world experience and more than 190,000 patients currently treated with DMF worldwide, it is a good timepoint to review the experience gathered so far and to re-evaluate the potential of this first-line oral multiple sclerosis (MS) drug. Post-hoc analyses of clinical and magnetic resonance imaging (MRI) data, some comprising more than 6 years of drug exposure including patients from the clinical trials, and the overall notion in clinical practice widely confirm the good efficacy of DMF in RRMS. Despite an overall good safety profile, it became also clear that the necessary clinical vigilance while using DMF may not be neglected. So far, four reported cases of progressive multifocal leukoencephalopathy (PML), a towering shadow over many MS therapies, warrant proper attention in newly-updated risk management plans. This review recapitulates efficacy and safety aspects of DMF therapy in relation to reported data from the pivotal clinical trials. In addition, we summarize recent insights into DMF mechanisms of action drawn from the field of basic research which may have important implications for clinical practice.
Avalon: an Alpha/Linux cluster achieves 10 Gflops for $15k (1998)

As an entry for the 1998 Gordon Bell price/performance prize, we present two calculations from the disciplines of condensed matter physics and astrophysics. The simulations were performed on a 70 processor DEC Alpha cluster (Avalon) constructed entirely from commodity personal computer technology and freely available software, for a cost of 152 thousand dollars.Avalon performed a 60 million particle molecular dynamics (MD) simulation of shock-induced plasticity using the SPaSM MD code. The beginning of this simulation sustained approximately 10 Gflops over a 44 hour period, and saved 68 Gbytes of raw data. The resulting price/performance is $15/Mflop, or equivalently, 67 Gflops per million dollars. This is more than a factor of three better than last year's Gordon Bell price/performance winners. This simulation is similar to those which won part of the 1993 Gordon Bell performance prize using a 1024-node CM-5. This simulation continued to run for a total of 332 hours on Avalon, computing a total of 1.12 x 1016 floating point operations. This puts it among the few scientific simulations to have ever involved more than 10 Petaflops of computation.Avalon also performed a gravitational treecode N-body simulation of galaxy formation using 9.75 million particles, which sustained an average of 6.78 Gflops over a 26 hour period. This simulation is exactly the same as that which won a Gordon Bell price/performance prize last year on the Loki cluster, at a total performance 7.7 times that of Loki, and a price/performance 2.6 times better than Loki. Further, Avalon ranked at 315th on the June 1998 TOP500 list, by obtaining a result of 19.3 Gflops on the parallel Linpack benchmark.
Chronic ulcer of the leg: clinical history. (1987)

Six hundred patients with chronic leg ulcers were studied by detailed history and examination as part of a population survey. In 22% ulceration began before the age of 40, and in this group the sex incidence was equal. Over age 40 there was an increasing preponderance of women. Ulcers were significantly more common in the left leg in women but not in men. The site of 26% of ulcers did not include the classical medial goiter area. The median duration of the ulceration at the time of the survey was nine months and 20% had not healed in over two years. The great majority of patients had had recurrence, 66% having had episodes of ulceration for more than five years. Healing of ulcers is a serious problem, but preventing recurrence is the greater challenge.
Total body irradiation as a method of preparation for bone marrow transplantation: a new technique and review. (1984)

Recently total body irradiation has been used as a method of preparation for the bone marrow transplantation. However, it has become apparent that total body irradiation is one of the causes of interstitial pneumonia. There are several factors in total body irradiation that may induce interstitial pneumonia. 1) Total dose. Usually 10 Gy which is used in America, reduces the survival of the leukemic cells to about 0.001% while the normal tissue cells are reduced to about 1%. However there is no evidence that 10 Gy is enough for bone marrow transplantation. 2) Dose rate. A dose of 10 Gy given at a high dose rate is about 10 times more effective than at a low dose rate. However, the dose-rate effects become less pronounced when the single dose is under 2 Gy. 3) Dose fractionation. The effect on cell survival of dose fractionation is discussed. Since the recovery from the radiation damage is usually greater in normal cells than in the malignant cells, fractionated radiation will have great advantages. 4) Dose distribution and new irradiation technique. It is difficult to obtain homogeneous distribution if a single large radiation field is used. In our hospital the patients receive total body irradiation by a moving treatment couch method under X-rays exposure. Homogeneous dose distribution is achieved with this method. Total body irradiation will become an almost routine treatment method for bone marrow transplantation with multifractionated irradiation.
Hydrolysis of triglyceride by the whole cell of Pseudomonas putida 3SK in two‐phase batch and continuous reactors systems (1994)

Batch and continuous hydrolysis of olive oil in an organic-aqueous two-phase system using the live whole cell of Pseudomonas putida 3SK as a source of a lipase is investigated. The strain was not only fully viable and grown well, but also produced extracellular lipase simultaneously. The degree of hydrolysis, depending on olive oil concentration in the solvents, was maximal at 13.5% (w/v) and decreased with the increase of the substrate concentration. At the optimal condition, a degree of hydrolysis higher than 95% was achieved with 24 h at 30 degrees C when the reaction was carried out in a two-phase batch stirred reactor. For long-term operation a continuous stirred reactor was designed. When the reaction was carried out in a continuous stirred reactor, the degree was hydrolysis reached 86% at a dilution rate of 0.2 h(-1). Satisfactory performance of a two-phase bioreactor was obtained in a long-term continous operation, which lasted for at least 30 days by feeding organic solvent containing olive oil and aqueous media separately. (c) 1994 John Wiley & Sons, Inc.
Difluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA Replication (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDifluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA ReplicationSean Moran, Rex X.-F. Ren, Rumney, and Eric T. KoolView Author Information Department of Chemistry and Department of Biochemistry and Biophysics University of Rochester, Rochester, New York 14627 Cite this: J. Am. Chem. Soc. 1997, 119, 8, 2056–2057Publication Date (Web):February 26, 1997Publication History Received25 October 1996Published online26 February 1997Published inissue 1 February 1997https://pubs.acs.org/doi/10.1021/ja963718ghttps://doi.org/10.1021/ja963718grapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views1791Altmetric-Citations287LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Adenine,Genetics,Monomers,Peptides and proteins,Selectivity Get e-Alerts
Molecular Genetics of Endocrine Disorders (1998)

Molecular genetics and inherited human disease, A.P. Read molecular pathology of pituitary hormone deficiency syndromes, J.S. Parks Kallmann's syndrome, E.I. Rugarli and A. Ballabio diabetes insipidus, B.A. van Oost and A.M.W. van den Ouweland molecular aetiology of thyroid cancers, M. Santoro, M. Grieco, A. Fusco and G. Vecchio thyroid hormone resistance syndromes, S.R. Weiss and R.E. Weiss molecular aetiology of parathyroid disorders, S.H.S. Pearce and R.V. Thakker cloning the gene causing Lowe's syndrome abnormalities of structure and function resulting from mutation in the steroid hormone receptors, G.R. Willams parathyroid hormone-related protein and the humoral hypercalcaemia of malignancy, T.J. Martin, J. Southby and M.T. Gillespie G-proteins, W.R. Schwindinger and M.A. Levine insulin receptor defects, A. Krook, P. Humphreys and S. O'Rahilly the genetics of adrenal steroid synthesis, G. Rumsby and J.W. Honour phaeochromocytomas, E.R. Maher and C. Eng molecular basis of human hypertension - role of angiotensinogen, P. Corvol, F. Soubrier and X. Jeunemaitre the control of gonadal cell death and aetiology of gonadal tumours, B.C.J.M. Fauser and A.J.W. Hsueh disorders of sexual development and infertility, I.A. Hughes the genetics of the hyperlipidaemic disorders, I.N.M. Day and S.E. Humphries immunogenetics, A.M. McGregor cloning of the calcitrophic receptors - parathyroid hormone and parathyroid hormone-related peptide, and calcitonin, H.M. Kronenberg, A.B. Abou-Samra, F.R. Bringhurst, T.J. Gardella, H. Juppner and G.V. Sergre adrenoleukodystrophy, H.W. Moser and K.D. Smith.