gebhart/patrepeval huggingface dataset:
Preparation of 4-Amino-3(2H)-pyridazinones by Direct Amination of 3(2H)-Pyridazinones with Hydrazine (1989)

Reaction of a variety of 3(2!)-pyridazinones with hydrazine hydrate gives moderate to excellent yields of the corresponding 4-amin0-3(ZH)-pyridazinones.We have been interested for some time in the synthesis of derivatives of aminophenyl and pyridyl substituted azinones and in their potential as cardiovascular agents, especially compounds structurally related to the positive inotropic agents amrinone, 3-amino-5-(4-pyridyl)-2(1H)-pyridinone, and milrinone, 3-cyano-6-methyl- 5-(4-pyridy1)-2(1E)-pyridinone.As part of an earlier programme directed towards the synthesis of the combined p-blocker/vasodilator prizidilol, 3-[2-(3-2butylamino-2-hydroxypropoxy)phenyl]-6-hydrzinopyridazine, the reported preparation' of 6-(2-hydroxypheny1)-3(2E)-pyridarinone Zb by reaction of 4 -( 2-hydroxyphenyl)-4-oxo-1,1,1-trichlor0-2-butanol 1 with hydrazine hydrate and an inorganic base in ethylene glycol was repeated.The reaction was successful, but in 2 attempts to optimise the production of 2b it was found that 4-amino-6-(2-hydroxy-NHZNH2 HOCH2CH20H base HO
High in vitro antimicrobial activity of synthetic antimicrobial peptidomimetics against staphylococcal biofilms (2008)

SAMPs had superior antimicrobial activity in staphylococcal biofilms compared with conventional antibiotics and are potential new therapeutic agents for biofilm-associated infections.
Molecular detection of prostate cancer in urine by GSTP1 hypermethylation. (2001)

Novel approaches for the early detection and management of prostate cancer are urgently needed. Clonal genetic alterations have been used as targets for the detection of neoplastic cells in bodily fluids from many cancer types. A similar strategy for molecular diagnosis of prostate cancer requires a common and/or early genetic alteration as a specific target for neoplastic prostate cells. Hypermethylation of regulatory sequences at the glutathione S-transferase pi (GSTP1) gene locus is found in the majority (>90%) of primary prostate carcinomas, but not in normal prostatic tissue or other normal tissues. We hypothesized that urine from prostate cancer patients might contain shed neoplastic cells or debris amenable to DNA analysis. Matched specimens of primary tumor, peripheral blood lymphocytes (normal control), and simple voided urine were collected from 28 patients with prostate cancer of a clinical stage amenable to cure. Genomic DNA was isolated from the samples, and the methylation status of GSTP1 was examined in a blinded manner using methylation-specific PCR. Decoding of the results revealed that 22 of 28 (79%) prostate tumors were positive for GSTP1 methylation. In 6 of 22 (27%) cases, the corresponding urine-sediment DNA was positive for GSTP1 methylation, indicating the presence of neoplastic DNA in the urine. Furthermore, there was no case where urine-sediment DNA harbored methylation when the corresponding tumor was negative. Although we only detected GSTP1 methylation in under one-third of voided urine samples, we have demonstrated that molecular diagnosis of prostate neoplasia in urine is feasible. Larger studies focusing on carcinoma size, location in the prostate, and urine collection techniques, as well as more sensitive technology, may lead to the useful application of GSTP1 hypermethylation in prostate cancer diagnosis and management.
Multimedia surveillance systems (2005)

The integration of video technology and sensor networks constitutes the fundamental infrastructure for new generations of multimedia surveillance systems, where many different media streams (audio, video, images, textual data, sensor signals) will concur to provide an automatic analysis of the controlled environment and a real-time interpretation of the scene. New solutions can be devised to enlarge the view of traditional surveillance systems by means of distributed architectures with fixed and active cameras, to enhance their view with other sensed data, to explore multi-resolution views with zooming and omnidirectional cameras. Applications regard surveillance of wide indoor and outdoor area and particularly people surveillance: in this case, multimedia surveillance systems can be enriched with biometric technology; the best views of detected persons and their extracted visual features (e.g. faces, voices, trajectories) can be exploited for people identification.VSSN05 is the third edition of the workshop, co-located at ACM Multimedia Conference, that embraces research reports on video surveillance and, since the edition of 2004, sensor networks. This paper gives a short overview of the hot topics in multimedia surveillance systems and introduces some research activities currently engaged in the world and presented at VSSN05.
Yield Evaluation of a Glyphosate‐Tolerant Soybean Line after Treatment with Glyphosate (1995)

Transformation of soybean [ Glycine max (L.) Merr.] with a gene encoding a glyphosate‐tolerance 5‐enolpyruvylshikimate‐3‐phosphate synthase enzyme from Agrobacterium sp. strain CP4 resulted in the development of glyphosate‐tolerant line 40‐3‐2. Glyphosate ( N ‐phosphonomethyl glycine) is the active ingredient of Roundup herbicide. Line 40‐3‐2 was yield tested at 17 locations in 1992,23 locations in 1993, and 18 locations in 1994. At those locations, broadcast applications of glyphosate at various rates were made over 40‐3‐2 or its derivatives from early vegetative growth to pod fill. No significant yield reduction was observed as a result of the glyphosate treatment at any of the locations. Development of glyphosate‐tolerant soybean promises to provide the farmer with access to a new weed control system that should result in lower production costs and reliable weed control under a wide range of conditions.
Laser-induced fluorescence detection of atmospheric NO(subscript)2 at parts per trillion mixing ratios (2001)

A laser-induced fluorescence instrument designed for continuous, autonomous, in situ observations of atmospheric NO2. The instrument sensitivity is 10 parts per trillion (ppt) in 10 seconds of integration (S/N=2) with a detection limit below 1 ppt.
Serum Magnesium Level and Clinical Deterioration in Alzheimer’s Disease (2007)

Our data suggest that there is a relationship between serum Mg levels and the degree of Alzheimer's disease and that the determination of the Mg level at various stages may provide valuable information in further understanding the progression and treatment of Alzheimer's disease.
Internal tandem duplication of the flt3 gene found in acute myeloid leukemia. (1996)

We analyzed mRNA expression of the flt3 gene in 30 patients with acute myeloid leukemia (AML) and 50 with acute lymphoblastic leukemia (ALL). Using reverse transcriptase-polymerase chain reaction (RT-PCR), expression of flt3 was observed in 61 patients; 22 (73%) with AML and 39 (78%) with ALL. Among these, five patients with AML (one M2, two M4, and two M5) showed unexpected longer transcripts with a primer combination which could amplify the transmembrane (TM) domain through the juxtamembrane (JM) domain. For those patients who expressed flt3 mRNA, the extracellular domain of the flt3 gene was also examined by RT-PCR, but no length abnormality was seen in this region. We further analyzed the TM domain through the second tyrosine kinase domain by genomic amplifications. The five patients who showed aberrant flt3 transcripts exhibited abnormal longer PCR products in addition to the germline products at a region corresponding to the JM through the first TK (TK1) domains. Sequence analyses of the abnormal RT-PCR products demonstrated that partial sequences were tandemly duplicated. Because all these altered transcripts were in-frame, deduced protein products could be expected. Sequence analyses of the genomic DNA revealed that three of the five patients showed a simple internal duplication within exon 11; one had an internal duplication (26 bp) with a 4-bp insertion; and in the fifth patient, a 136-bp sequence from the 3' part of exon 11 to intron 11 and the first 16-bp sequence of exon 12 were each duplicated with 1-bp insertion. In order to confirm the tumor specificity of these alterations, DNA samples obtained at complete remission were also analyzed in the three patients harboring an flt3 duplication, but no abnormal PCR product other than germline was detected in any of the samples. Our results suggest that an internal tandem duplication at the JM/TK1 domains of the flt3 gene is a somatic change detected preferentially in AML, possibly containing a monocytic component.
On‐line size measurement of yeast aggregates using image analysis (2001)

Alcohol fermentation productivity can be strongly improved using a flocculation-based yeast recycle. However, the efficiency of the biomass retention system depends strongly on the yeast particle size. Accordingly, the monitoring and control of yeast floc diameter are of primary importance. The on-line measurement of mean floc diameter has been achieved using on-line image analysis, based on the evaluation of image texture. The texture analysis method consisted in the building of a co-occurrence matrix from which the so-called "Energy parameter" was extracted. While image texture is usually used for classification purposes, it has been used here as a quantitative descriptor: a correlation has been found between this statistical image feature and off-line manual floc-size determinations. In the floc-size range investigated (X 0.5-4.3 mm), the evaluated mean diameter was in good agreement with the actual particle size, with a determination coefficient equal to 0.980. In contrast with manual measurements, slow and tedious, this method gave the value of the mean particle diameter in real-time, without sampling. This novel tool has been used to investigate the behavior of yeast aggregates as a function of fermentation conditions. While biomass concentration was kept constant, step increases of the feed rate led to a decrease of the mean floc diameter. Image analysis showed that the particle-size reduction could occur within a few minutes after modification of the medium dilution rate, demonstrating the disruptive effect of the CO(2) efflux. The kinetic of aggregate formation was dependent on the gas-phase composition. Instead of recycling fermentation gas, sparging the fermentor with nitrogen, to reduce dissolved CO(2) concentration, increased the rate of floc-size growth.
Raf‐1 is activated by the p38 mitogen‐activated protein kinase inhibitor, SB203580 (1999)

SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentrations of SB203580 required lie above those necessary to inhibit p38 MAPK and we were unable to detect basal levels of active p38 MAPK. SB203580 does not directly activate Raf-1 in vitro, and fails to activate Ras, MEK, and ERK in intact cells. In vitro, however, SB203580-stimulated Raf-1 activates MEK1 in a coupled assay. We conclude that activation of Raf-1 by SB203580 is not mediated by an inhibition of p38 MAPK, is Ras-independent, and is uncoupled from MEK/ERK signaling.
A PROSPECTIVE ANALYSIS OF 1,930 PATIENTS WITH HEMATURIA TO EVALUATE CURRENT DIAGNOSTIC PRACTICE (2000)

Our findings suggest that cystoscopy cannot be safely avoided even in younger patients with microscopic hematuria. Only a combination of ultrasound and IVP detected all upper tract tumors.
Epitopes of group A streptococcal M protein that evoke cross-protective local immune responses (1992)

The present studies were undertaken to identify conserved epitopes of group A streptococcal M proteins that evoke cross-protective mucosal immune responses. Two synthetic peptides copying conserved regions of type 5 M protein, designated SM5(235-264)C and SM5(265-291)C, were covalently linked to carrier molecules and their immunogenicity was tested in laboratory animals. Rabbit antisera against both peptides cross-reacted with multiple serotypes of group A streptococci, indicating that the peptides contained broadly cross-reactive, surface exposed M protein epitopes. Serum antipeptide antibodies adsorbed to the surface of heterologous type 24 streptococci passively protected mice against intranasal challenge infections. Mice that were actively immunized intranasally with each synthetic peptide covalently linked to the B subunit of cholera toxin were protected against colonization and death after intranasal challenge infections with type 24 streptococci in the absence of serum opsonic antibodies. These data confirm and extend previous observations that conserved M protein epitopes evoke cross-protective local immunity and may serve as the basis for broadly cross-protective M protein vaccines.
Ganglioside GM2 expression on human melanoma cells correlates with sensitivity to lymphokine‐activated killer cells (1989)

Ganglioside GM2 is expressed on cell surface membranes of a variety of human malignant cells and has been demonstrated to be immunogenic in humans. We have assessed the role of the antigen GM2 on melanoma cells as a recognition structure for lymphokine-activated killer (LAK) cells. LAK cells were generated by stimulation of non-adherent peripheral blood lymphocytes (PBL) from human donors with recombinant interleukin-2 (IL-2). The selection of target cells was based on GM2 content and included 11 human melanoma cell lines and 2 human leukemia lines. Using a single-cell binding assay, LAK cell binding to target lines expressing high levels of GM2 was significantly greater than to those expressing minimum GM2. This cell-binding was specifically inhibited by addition of purified GM2 but not by other gangliosides. LAK-melanoma cell-binding was also specifically inhibited by anti-GM2 monoclonal antibody (MAb). For further analysis LAK cell lysis of melanoma target cells expressing various amounts of GM2 was assessed. A significant correlation occurred with GM2 expression and LAK cell lysis (p less than 0.025; r = 0.623). Three other gangliosides commonly expressed on human melanoma, GM3, GD3 and GD2, had no correlation with LAK cell lysis. These studies suggest that GM2 on melanoma cells is a marker for LAK cell sensitivity, as well as indicate that GM2 is a potential target recognition structure for human LAK cells.
Biomimetic sound-source localization (2002)

Sound-source localization systems typically comprise free-field microphone arrays. In nature, directional acoustic sensing evolved to rely on diffraction about the head with only two ears. For localization, the brain uses the resultant frequency-dependent acoustic phase and intensity differences between the two ears. We conceive a biomimetic artificial head with microphones placed on its surface. The interaural functions can be computed analytically by modeling the head as a sphere. We define a suitable metric between interaural functions, whose global minimum provides the true source direction. The natural configuration in which the two sensors are placed antipodally on the sphere has intrinsic rotational symmetry: it allows localization only up to a circle around the interaural axis. We describe two methods for breaking the detrimental symmetry in order to achieve full spherical localization capability. First, we consider rotation of the apparatus relative to the source and the information it adds to the localization metric. We derive analytically the gradient of the pressure field under rotation and compute the induced acoustic flow on the interaural localization functions. Second, we explore placing the sensors in configurations differing from antipodal. We show the efficacy of these methods through simulations.
Chemoprevention of nonalcoholic fatty liver disease by dietary natural compounds (2013)

Nonalcoholic fatty liver disease (NAFLD) refers to a wide spectrum of liver disease that is not from excess alcohol consumption, but is often associated with obesity, type 2 diabetes, and metabolic syndrome. NAFLD pathogenesis is complicated and involves oxidative stress, lipotoxicity, mitochondrial damage, insulin resistance, inflammation, and excessive dietary fat intake, which increase hepatic lipid influx and de novo lipogenesis and impair insulin signaling, thus promoting hepatic triglyceride accumulation and ultimately NAFLD. Overproduction of proinflammatory adipokines from adipose tissue also affects hepatic metabolic function. Current NAFLD therapies are limited; thus, much attention has been focused on identification of potential dietary substances from fruits, vegetables, and edible plants to provide a new strategy for NAFLD treatment. Dietary natural compounds, such as carotenoids, omega-3-PUFAs, flavonoids, isothiocyanates, terpenoids, curcumin, and resveratrol, act through a variety of mechanisms to prevent and improve NAFLD. Here, we summarize and briefly discuss the currently known targets and signaling pathways as well as the role of dietary natural compounds that interfere with NAFLD pathogenesis.
Fetal infections and antibody profiles in pigs naturally infected with porcine circovirus type 2 (PCV2). (2012)

The aim of this study was to describe early infections with porcine circovirus type 2 (PCV2) in naturally infected piglets and the piglets' serologic profiles. A total of 20 sows (15 PCV2-vaccinated and 5 unvaccinated) and 100 newborn piglets were studied. Colostrum and serum of the sows and serum of the presuckling piglets were obtained on the day of parturition. Milk samples were collected on day 20 postpartum. Blood samples were taken and the piglets weighed on days 1, 20, 42, 63, and 84 postpartum. Colostrum and milk were evaluated for infectious PCV2 and for PCV2 total antibody (TA), neutralizing antibody (NA), and IgA. Serum samples were evaluated for PCV2 TA, NA, IgA, IgM, and DNA. The sows had high levels of TA and NA in serum and colostrum; however, 11 and 5, respectively, of the 20 colostrum and milk samples contained infectious PCV2. In the serum, PCV2 DNA and IgM were detected in 17 and 5, respectively, of the 20 sows. Nine piglets were born with PCV2 antibodies, which indicates in utero transmission of PCV2 after the period of immunocompetence (> 70 d of gestation). On day 1 postpartum, PCV2 DNA was detected in 29 of the 100 serum samples from the piglets. There was no difference between the weights of viremic and nonviremic piglets throughout the study. In conclusion, even on farms with sows that have high PCV2 antibody titers, vertical transmission of PCV2 may occur, resulting in piglet infection.
Pathogenic anti-DNA autoantibody-inducing T helper cell lines from patients with active lupus nephritis: isolation of CD4-8- T helper cell lines that express the gamma delta T-cell antigen receptor. (1990)

The antigen responsible for autoimmunization in systemic lupus erythematosus is unknown. In spite of this obstacle, we show that T helper (Th) cell lines that are functionally relevant to this disease can be established in vitro. We derived a total of 396 interleukin 2-dependent T-cell lines from the in vivo activated T cells of five patients with lupus nephritis. Only 59 (approximately 15%) of these lines had the ability to selectively augment the production of pathogenic anti-DNA autoantibodies that were IgG in class, cationic in charge, specific for native DNA, and clonally restricted in spectrotype. Forty-nine of these autoantibody-inducing Th lines were CD4+ and expressed the alpha beta T-cell receptor (TCR). The other 10 were CD4-8- (double negative), 3 expressing the alpha beta TCR and 7 expressing the gamma delta TCR. All of the autoantibody-inducing Th lines responded to some endogenous antigen presented by autologous B cells. The autoreactive responses of the CD4+ Th lines were restricted to HLA class II antigens, whereas those of the double-negative cells were not. Endogenous heat shock or stress proteins of the HSP60 family that were expressed by the lupus patients' B cells were involved in stimulating an autoreactive proliferation of the gamma delta Th cells. These studies demonstrate a novel helper activity of certain gamma delta T cells in a spontaneous autoimmune response.
Treatment of Periodontal Disease in Diabetics Reduces Glycated Hemoglobin (1997)

Periodontal disease is a common infection-induced inflammatory disease among individuals suffering from diabetes mellitus. The purpose of this study was to assess the effects of treatment of periodontal disease on the level of metabolic control of diabetes. A total of 113 Native Americans (81 females and 32 males) suffering from periodontal disease and non-insulin dependent diabetes mellitus (NIDDM) were randomized into 5 treatment groups. Periodontal treatment included ultrasonic scaling and curettage combined with one of the following antimicrobial regimens: 1) topical water and systemic doxycycline, 100 mg for 2 weeks; 2) topical 0.12% chlorhexidine (CHX) and systemic doxycycline, 100 mg for 2 weeks; 3) topical povidone-iodine and systemic doxycycline, 100 mg for 2 weeks; 4) topical 0.12% CHX and placebo; and 5) topical water and placebo (control group). Assessments were performed prior to and at 3 and 6 months after treatment and included probing depth (PD), clinical attachment level (CAL), detection of Porphyromonas gingivalis in subgingival plaque and determination of serum glucose and glycated hemoglobin (HbA1c). After treatment all study groups showed clinical and microbial improvement. The doxycycline-treated groups showed the greatest reduction in probing depth and subgingival Porphyromonas gingivalis compared to the control group. In addition, all 3 groups receiving systemic doxycycline showed, at 3 months, significant reductions (P < or = 0.04) in mean HbA1c reaching nearly 10% from the pretreatment value. Effective treatment of periodontal infection and reduction of periodontal inflammation is associated with a reduction in level of glycated hemoglobin. Control of periodontal infections should thus be an important part of the overall management of diabetes mellitus patients.
Conformational analysis of polysaccharides: stereochemical significance of different linkage positions in β-linked polysaccharides (1969)

For homopolysaccharides in which the glycosidic oxygen is equatorial to both sugar rings, computer model building suggests that linkage positions have the following stereochemical and biological significance: 1,4-linkages lead to stiff ribbon-like chains; 1,3-linkages to stiff helices likely to combine in multiply stranded ropes; 1,6-linkages form loosely jointed chains with high flexibility and solubility; 1,2-linkages lead to stiff contorted chains unlikely to form stable tertiary structures.
Real three-dimensional microstructures fabricated by photopolymerization of resins through two-photon absorption (2000)

Effective energy windows for two-photon absorption (TPA) photopolymerization of resins were investigated and, with a properly selected laser pulse energy, exquisite three-dimensional (3D) microstructures with submicrometer spatial resolution were achieved. The results show the inherent utility of TPA in the fabrication of real 3D patterns. In particular, we propose and utilize a resin pre-exposure technique by which freely movable components affixed to an axle are built, demonstrating a new application of TPA in laser microfabrication.