gebhart/patrepeval huggingface dataset:
Field tests designed to demonstrate increased spectral efficiency for personal communications (2002)

Field tests were conducted to test whether a broadband code division multiple access (CDMA) network can be overlayed on the 1850-1990-MHz band which will not interfere with existing FSM (fixed site microwave) users of that band and will itself function properly. The test demonstrated that, on the average, even without the use of a notch filter and without voice activity, there will be no significant interference to microwave receivers under EIA specification 10E when 50 users are located in single cells of diameter 1200 ft. The CDMA system functioned with excellent voice quality at mobile power levels of 100 mu W or less, and the microwave signals caused no significant effect on performance. Additionally, the tests obtained data on propagation conditions in the 1850-1990-MHz band in urban, rural, suburban and in-building areas.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The MDS1–EVI1 Gene Complex as a Retrovirus Integration Site: Impact on Behavior of Hematopoietic Cells and Implications for Gene Therapy (2008)

Gene therapy trials have been performed with virus-based vectors that have the ability to integrate permanently into genomic DNA and thus allow prolonged expression of corrective genes after transduction of hematopoietic stem and progenitor cells. Adverse events observed during the X-linked severe combined immunodeficiency gene therapy trial revealed a significant risk of genotoxicity related to retrovirus vector integration and activation of adjacent proto-oncogenes, with several cases of T-cell leukemia linked to vector activation of the LMO2 gene. In patients with chronic granulomatous disease (CGD), rhesus macaques, and mice receiving hematopoietic stem and progenitor cells transduced with retrovirus vectors, a highly non-random pattern of vector integration has been reported. The most striking finding has been overrepresentation of integrations in one specific genomic locus, a complex containing the MDS1 and the EVI1 genes. Most evidence suggests that this overrepresentation is primarily due to a modification of primitive myeloid cell behavior by overexpression of EVI1 or MDS1-EVI1, as opposed to a specific predilection for integration at this site. Three different proteins can be produced from this complex locus: MDS1, MDS1-EVI1, and EVI1. This review will summarize current knowledge regarding this locus and its gene products, with specific focus on issues with relevance to gene therapy, leukemogenesis, and hematopoiesis. Insights into the mechanisms that result in altered hematopoiesis and leukemogenesis when this locus is dysregulated could improve the safety of gene therapy in the future.
Sympathetic and parasympathetic interaction in vascular control of the nasal mucosa in anaesthetized cats. (1994)

1. In cats anaesthetized with pentobarbitone sodium (45 mg kg-1), electrical stimulation of the parasympathetic nerve fibres to the nasal mucosa evoked frequency-dependent increases in nasal arterial blood flow whereas stimulation of the superior cervical sympathetic nerve induced marked vasoconstriction. 2. Sympathetic nerve stimulation for 3 min at 10 Hz evoked significant (P < 0.05) and prolonged (> 30 min) attenuation of the vasodilatory response to subsequent parasympathetic stimulation. 3. Combined pretreatment with adrenergic and cholinergic blockers reduced the vasoconstrictory effect of sympathetic stimulation by 28 +/- 4% (mean +/- S.E.M.) and the parasympathetically evoked vasodilatation by 20 +/- 5%. 4. The vasodilatory effects of exogenous vasoactive intestinal polypeptide, peptide histidine isoleucine and galanin, and the vasoconstrictory effects of exogenous neuropeptide Y (NPY) and alpha,beta-methylene adenosine 5'-triphosphate were not altered by adrenoceptor antagonists and atropine whereas the effects of exogenous noradrenaline and acetylcholine were virtually abolished. 5. The atropine-resistant parasympathetic vasodilatation remained significantly attenuated for more than 30 min after the non-adrenergic sympathetically evoked vasoconstriction. 6. Exogenous NPY (25 x 10(-9) mol) mimicked the effect of sympathetic stimulation in attenuating subsequent parasympathetically evoked vasodilatation.
Serum Immunoreactive-Leptin Concentrations in Normal-Weight and Obese Humans (1996)

Serum leptin concentrations are correlated with the percentage of body fat, suggesting that most obese persons are insensitive to endogenous leptin production.
Multiplicity of DNA end resection machineries in chromosome break repair (2009)

DNA end resection is critical for chromosome break repair by homologous recombination and influences the efficiency of repair by nonhomologous DNA end joining. An elegant study by Sinha and colleagues (pp. 1423-1437) published in the June 15, 2009, issue of Genes & Development identified a novel mycobacterial DNA end resection protein complex, AdnAB, that harbors dual DNA motor and dual nuclease functions. Sinha and colleagues also demonstrated that the DNA end-binding protein complex Ku regulates the activity of AdnAB.
Enhanced Reporter Gene Assay for the Detection of Osteogenic Differentiation (2010)

Detection of osteogenic differentiation is crucial for bone tissue engineering. Despite established standard end point assays, there is increasing demand for methods allowing noninvasive kinetic differentiation monitoring. Reporter gene assays employing tissue-specific promoters and suitable reporter genes fulfill these requirements. Many promoters, however, exhibit only weak cis-activating potential, thus limiting their application to generate sensitive reporter gene assays. Therefore, the aim of this study was to design a reporter gene assay employing elements of the murine osteocalcin promoter coupled to a viral enhancer for signal amplification. Additionally, the system's practicability was enhanced by introducing a secreted luciferase as a quantifiable reporter gene. The constructs were tested in C2C12 cells stimulated with recombinant human bone morphogenetic protein 2 for osteogenic differentiation in two-dimensional and three-dimensional culture. Osteogenic differentiation was confirmed by standard assays for osteogenesis. The reporter gene signal was detected through a secreted luciferase or fluorescence microscopy for enhanced yellow fluorescent protein. The constructs exhibited strong activation upon treatment with recombinant human bone morphogenetic protein 2. Weak background expression was observable in negative controls, attributed to the pan-active viral enhancer. In conclusion, a novel enhancer/tissue-specific promoter combination allows specific signal-amplified, kinetic monitoring of osteogenic differentiation in a nonsample-destructive manner.
A Novel Site-Targeted Ultrasonic Contrast Agent With Broad Biomedical Application (1996)

These data provide the first in vivo demonstration of a site-specific ultrasonic contrast agent and have potential for improved sensitivity and specificity for noninvasive diagnosis of thrombi and other pathological diseases.
Viral Determinants of Resistance to Treatment in Patients with Hepatitis C (2007)

Chronic hepatitis C virus (HCV) infection affects more than 170 million persons worldwide and is responsible for the development of liver cirrhosis in many cases. Standard treatment with pegylated alpha interferon (IFN-alpha) in combination with the nucleoside analogue ribavirin leads to a sustained virologic response in approximately half of the patients. IFN-alpha is classified as an indirect treatment, as it interacts with the host's immune response. The mechanism of action of ribavirin is still unknown. The benefit of triple therapy by adding other antiviral agents, e.g., amantadine, is controversial. Currently, new direct antiviral drugs (HCV protease/polymerase inhibitors) are being evaluated in phase 1/phase 2 trials. Phenotypic resistance to antiviral therapy has been attributed to amino acid variations within distinct regions of the HCV polyprotein. While sensitivity to IFN-alpha-based antiviral therapy in vivo is clearly correlated with the number of mutations within the HCV NS5A protein, the underlying functional mechanisms for this association are unknown. In turn, in vitro, several mechanisms to circumvent the host immune defense or to block treatment-induced antiviral activities have been described for different HCV proteins. By the introduction of direct antiviral drugs, hepatitis C therapy now is entering a new era in which the development of resistance may become the most important parameter for treatment success or failure.
Detecting Wireless LAN MAC Address Spoofing (2003)

An attacker wishing to disrupt a wireless network has a wide arsenal available to them. Many of these tools rely on using a faked MAC address, masquerading as an authorized wireless access point or as an authorized client. Using these tools, an attacker can launch denial of service attacks, bypass access control mechanisms, or falsely advertise services to wireless clients. This presents unique opportunities for attacks against wireless networks that are difficult to detect, since the attacker can present himself as an authorized client by using an altered MAC address. As nearly all wireless NICs permit changing their MAC address to an arbitrary value – through vendor-supplied drivers, open-source drivers or various application programming frameworks – it is trivial for an attacker to wreak havoc on a target wireless LAN. This paper describes some of the techniques attackers utilize to disrupt wireless networks through MAC address spoofing, demonstrated with captured traffic that was generated by the AirJack, FakeAP and Wellenreiter tools. Through the analysis of these traces, the author identifies techniques that can be employed to detect applications that are using spoofed MAC addresses. With this information, wireless equipment manufacturers could implement anomalybased intrusion detection systems capable of identifying MAC address spoofing to alert administrators of attacks against their networks.
Predictors of sudden cardiac death change with time after myocardial infarction: results from the VALIANT trial (2009)

Predictors of SCD change with time after MI. Future studies of risk stratification for SCD should account for changes in these factors with time after MI.
On Joint Optimization of Motion Compensation, Quantization and Baseline Entropy Coding in H.264 with Complete Decoder Compatibility (2006)

The paper presents a framework for jointly designing motion compensation, quantization and entropy coding in a hybrid video coding structure to minimize a rate distortion cost. Given motion compensation, a soft decision-based quantization algorithm is first designed to reduce the rate distortion cost by adapting quantization outputs to the baseline entropy coding method in the newest standard H.264. Motion compensation is then optimized by searching for a prediction to reduce the rate distortion cost further based on given quantization outputs. By alternating these two steps, an iterative method is then proposed. The proposed algorithms have been implemented based on the reference encoder of H.264 with complete baseline decoder compatibility. Comparative studies show that the baseline-based iterative optimization method achieves coding performance comparable, or sometimes superior, to that afforded by the main profile encoder.
VH/VL interface engineering to promote selective expression and inhibit conformational isomerization of thrombopoietin receptor agonist single-chain diabody (2010)

Thrombopoietin receptor agonist humanized VB22B single-chain diabody (hVB22B (scFv)(2)) was found to be expressed as a mixture of two conformational isomers, a single-chain diabody form and a bivalent scFv form, which had different V(H)/V(L) (variable region of the heavy chain/light chain) association patterns. The single-chain diabody form showed significantly higher biological activity than the bivalent scFv form and, when incubated at elevated temperatures, exhibited novel isomerization to the inactive bivalent scFv form. Therefore, therapeutic development of hVB22B (scFv)(2) would require separation of the purified single-chain diabody form from the mixture of the two conformational isomers and also inhibition of isomerization into an inactive bivalent scFv form during storage. Novel V(H)/V(L) interface engineering in hVB22 (scFv)(2), in which hydrogen bonding between H39 and L38 was substituted with electrostatic interaction to enhance the desired V(H)/V(L) association and inhibit the undesired V(H)/V(L) association, enabled selective expression of the desired conformational isomer without any reduction in biological activity or thermal stability. Moreover, V(H)/V(L) interface-engineered hVB22 (scFv)(2) was completely resistant to isomerization. Because the hydrogen bonding interaction between H39 and L38 and the surrounding residues are highly conserved in human antibody sequences, V(H)/V(L) interface engineering could be generally applied to various (scFv)(2) molecules for selective expression and inhibition of the isomerization of conformational isomers.
High-speed mapping of synaptic connectivity using photostimulation in Channelrhodopsin-2 transgenic mice (2007)

To permit rapid optical control of brain activity, we have engineered multiple lines of transgenic mice that express the light-activated cation channel Channelrhodopsin-2 (ChR2) in subsets of neurons. Illumination of ChR2-positive neurons in brain slices produced photocurrents that generated action potentials within milliseconds and with precisely timed latencies. The number of light-evoked action potentials could be controlled by varying either the amplitude or duration of illumination. Furthermore, the frequency of light-evoked action potentials could be precisely controlled up to 30 Hz. Photostimulation also could evoke synaptic transmission between neurons, and, by scanning with a small laser light spot, we were able to map the spatial distribution of synaptic circuits connecting neurons within living cerebral cortex. We conclude that ChR2 is a genetically based photostimulation technology that permits analysis of neural circuits with high spatial and temporal resolution in transgenic mammals.
Fast phase imaging in liquids using a rapid scan atomic force microscope (2006)

The authors report on fast phase imaging in liquids achieved by fast phase detection in rapid scan atomic force microscopy. The phase-shift images clearly revealed the compositional heterogeneities in styrene-butadiene-styrene block copolymer films even at an imaging rate of less than 100ms∕frame. They found that the contrast variations of the phase images depended on the phase-shift detection timing within a single oscillation cycle. The phase contrast increased as the tip approached the surface in each oscillation during imaging, while it decreased as the tip withdrew from the surface.
Freeze-Drying and Lyopreservation of Diblock and Triblock Poly(Lactic Acid)–Poly(Ethylene Oxide) (PLA–PEO) Copolymer Nanoparticles (2000)

In this study, the formulation and process parameters that determine successful production and long-term stability of freeze-dried poly(lactic acid) (PLA) nanoparticles with "hairy-like" poly(ethylene oxide) (PEO) surfaces were investigated. Nanoparticles with grafted (covalently bound) PEO coatings were produced by the salting-out method from blends of PLA and PLA-PEO diblock or triblock copolymers. PLA nanoparticles with physically adsorbed PEO were also produced. The redispersibility of the nanoparticles after freeze-drying under various conditions was assessed. The surface of the nanoparticles was characterized and classified in terms of "brush" and "loop" conformations. Upon freeze-drying, it appeared that the presence of PEO at the nanoparticle surface could severely impair the redispersibility of the particles, especially in the PEO-grafted systems. This effect was shown to be related to the amount and molecular weight of PEO in the various formulations. In most cases, particle aggregation was prevented by use of trehalose as lyoprotective agent. Increasing the concentration of particles in the suspension to be freeze-dried was shown to induce much less damage to the nanoparticles, and freezing the suspension at a very low temperature (-196 degrees C) was found to further improve the lyoprotective effect. Most of the lyoprotected nanoparticles remained stable for at least 12 weeks at 4 and -25 degrees C. The production and preservation of freeze-dried PLA-PEO diblock and triblock copolymer nanoparticles is feasible under optimized lyoprotective conditions.
Inhibition of the herpes simplex virus thymidine kinase gene transfection in Ltk-cells by potential Z-DNA forming polymers (1985)

It has been demonstrated that certain alternating purine and pyrimidine sequences may assume a left-handed Z-DNA conformation. In order to evaluate the possibility that Z-DNA is involved in the modulation of gene expression, we examined the ability of various synthetic DNA polymers to affect the transfection of herpes simplex virus thymidine kinase (HSVtk) gene in Ltk- cells using the DNA-calcium phosphate cotransfection technique. We found that potential Z-DNA forming polymers such as, poly(dG-m5dC) X poly(dG-m5dC) and poly(dG-dC) X poly(dG-dC), cotransfected with the tk gene decreased the level of Tk+ transformed colonies. In contrast, cotransfection of the tk gene with polymers which do not assume Z-conformation such as, poly(dG) X poly(dC) or poly(dA-dT) X poly(dA-dT) showed no effect on the number of colonies formed. About 50% inhibition of the Tk+ colony formation was obtained by 0.4 micrograms of poly(dG-m5dC) X poly(dG-m5dC), or by 2 micrograms of poly(dG-dC) X poly(dG-dC). DNA uptake into Ltk- cells was not significantly affected by any of these polymers. Approximately 20-42 base pairs (bp) long alternating dG-dC sequence linked at either the 5'-end or 3'-end of tk gene were cloned into plasmids. These recombinant plasmids, however, showed no remarkable effect upon the transfection of Ltk- cells. The DNAs of Tk+ colonies obtained by transfecting these recombinant plasmids were digested with BssH II and analyzed by Southern blotting. We demonstrated that the dG-dC sequences proximal to the tk gene were integrated into cellular DNA. All the presented results indicate that only larger polymers with the potential to assume a Z-DNA conformation may affect tk gene transfection either by inhibiting transcription or more probably by affecting the stable integration of the tk gene into the host chromosome.
Site-directed mutagenesis of tyrosine hydroxylase. Role of serine 40 in catalysis. (1992)

We have investigated the role of serine 40 (Ser-40) in tyrosine hydroxylase (TH) catalysis of basal and activated enzymes by protein kinase A (PKA)-mediated phosphorylation. Wild type and mutant TH were transiently and stably expressed in AtT-20 cells, and the enzymatic activities of the recombinant enzymes were analyzed. The specific enzymatic activity of transiently expressed TH mutants Ser-40-->leucine or-->tyrosine (Leu-40m or Tyr-40m) was higher than that of the wild type enzyme or of other mutants in which Ser-8, -19, and -31 were replaced by leucine. The kinetic studies carried out with the stably expressed TH show that the Km for the cofactor 6-methyltetrahydropterine is lower and the Ki for dopamine is higher when the enzymatic hydroxylation is catalyzed by the Leu-40m or Tyr-40m than by the wild type enzyme. The kinetic parameters and the pH profile of the enzymatic hydroxylation catalyzed by the Leu-40m or Tyr-40m are similar to the enzyme activated by PKA-mediated phosphorylation. We suggest that Ser-40 in TH exerts an inhibitory influence on the enzymatic activity, and its replacement with another amino acid by site-directed mutagenesis or its modification by phosphorylation leads to a change in conformation with an increased enzymatic activity. The importance of Ser-40 in the activation of TH by PKA-mediated phosphorylation was investigated by comparing the activation of the wild type enzyme with that of Leu-40m or Tyr-40m. The findings that the enzymatic activity is increased by PKA-mediated phosphorylation of the wild type enzyme, but not of the Leu-40m or Tyr-40m, demonstrate that phosphorylation at Ser-40 is essential for activation of TH by PKA. The findings that addition of ATP plus cAMP to homogenates from transfected AtT-20 cells stimulates the recombinant wild type TH activity indicate that these cells contain endogenous cAMP-dependent protein kinase.
Viscoelastic Properties of Intervertebral Disc Cells (1999)

The findings of this study provide new evidence for the existence of two biomechanically distinct cell populations in the intervertebral disc. These differences in mechanical behavior may be related to observed differences in the cytoskeletal architecture between these cells, and may further play an important role in the development, maintenance, and degeneration of the intervertebral disc.
Altered Cerebrospinal Fluid Neuropeptide Y and Peptide YY Immunoreactivity in Anorexia and Bulimia Nervosa (1990)

The related central nervous system peptides neuropeptide Y and peptide YY have been found to be among the most potent endogenous stimulants of feeding behavior. We measured these neuropeptides in cerebrospinal fluid to determine whether they contributed to the pathophysiologic characteristics of anorexia and bulimia nervosa. Cerebrospinal fluid neuropeptide Y concentrations were significantly elevated in underweight anorectic patients and in many of the anorectic patients studied at intervals after weight restoration. These levels normalized in long-term weight-restored anorectic patients who had a return of normal menstrual cycles. Increased neuropeptide Y activity may contribute to several characteristic disturbances in anorexia, including menstrual dysregulation. Cerebrospinal fluid peptide YY concentrations were significantly elevated in normal-weight bulimic patients abstinent from pathological eating behavior for a month compared with themselves when actively bingeing and vomiting or compared with healthy volunteers. Increased peptide YY activity may contribute to a drive to overfeed in normal-weight bulimic patients.
Old Testament Theology: Essays on Structure, Theme, and Text [review] / Walter Brueggemann; edited by Patrick D. Miller. (1994)

In this book, Walter Brueggemann, the well-known Old Testament commentator and theologian, has assembled fifteen of his essays (all but one of which have appeared previously in various journals and volumes) dealing with Old Testament theology.Along with other prominent scholars, such as
Transmitter diversity for OFDM systems and its impact on high-rate data wireless networks (1999)

Transmitter diversity and down-link beamforming can be used in high-rate data wireless networks with orthogonal frequency division multiplexing (OFDM) for capacity improvement. We compare the performance of delay, permutation and space-time coding transmitter diversity for high-rate packet data wireless networks using OFDM modulation. For these systems, relatively high block error rates, such as 10%, are acceptable assuming the use of effective automatic retransmission request (ARQ). As an alternative, we also consider using the same number of transmitter antennas for down-link beamforming as we consider for transmitter diversity. The investigation indicates that delay transmitter diversity with quaternary phase-shift keying (QPSK) modulation and adaptive antenna arrays provides a good quality of service (QoS) with low retransmission probability, while space-time coding transmitter diversity provides high peak data rates. Down-link beamforming together with adaptive antenna arrays, however, provides a higher capacity than transmitter diversity for typical mobile environments.