gebhart/patrepeval huggingface dataset:
p53 Mutations in Human Cancers (1991)

Mutations in the evolutionarily conserved codons of the p53 tumor suppressor gene are common in diverse types of human cancer. The p53 mutational spectrum differs among cancers of the colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues. Analysis of these mutations can provide clues to the etiology of these diverse tumors and to the function of specific regions of p53. Transitions predominate in colon, brain, and lymphoid malignancies, whereas G:C to T:A transversions are the most frequent substitutions observed in cancers of the lung and liver. Mutations at A:T base pairs are seen more frequently in esophageal carcinomas than in other solid tumors. Most transitions in colorectal carcinomas, brain tumors, leukemias, and lymphomas are at CpG dinucleotide mutational hot spots. G to T transversions in lung, breast, and esophageal carcinomas are dispersed among numerous codons. In liver tumors in persons from geographic areas in which both aflatoxin B1 and hepatitis B virus are cancer risk factors, most mutations are at one nucleotide pair of codon 249. These differences may reflect the etiological contributions of both exogenous and endogenous factors to human carcinogenesis.
The RGD containing site of the mouse laminin A chain is active for cell attachment, spreading, migration and neurite outgrowth (1991)

The laminin A chain has been sequenced by cDNA cloning and was found to contain an RGD sequence. Synthetic peptides containing the RGD sequence and flanking amino acids were active in mediating cell adhesion, spreading, migration, and neurite outgrowth. Furthermore, endothelial cell attachment to a laminin substrate was inhibited by an RGD-containing synthetic peptide. Antisera against the integrin (fibronectin) receptor, and monoclonal antibody to the integrin, VLA-6, inhibited cell interaction with laminin, as well as with peptides containing an RGD sequence. These results suggest that the RGD containing site of laminin is active and interacts with the integrin family of receptors in certain cells.
Detection of polymorphisms using thermal cycling with a single oligonucleotide on a DNA sequencing gel (1993)

A method is described for the detection of restriction fragment length polymorphisms (RFLPs) in single copy genes in mammalian cells using one 5'-labelled oligonucleotide. This linear amplification (LA) method employs a single oligonucleotide as primer, which is extended by Taq DNA polymerase up to a restriction enzyme cleavage site. The products are arithmetically amplified by thermal cycling. The size of the products are determined by the sequence of the oligonucleotide and the position of the restriction enzyme cleavage site. Hence, an RFLP can be observed by measuring the size of the products. Polymorphisms which differ in size by a small number of base pairs, as are found in (CA)n repeats, are especially suitable for analysis by the LA procedure since the products are run on DNA sequencing gels. A number of genes were examined by the procedure and all produced a satisfactory signal including GC-rich template. It is proposed that the LA method would be suitable for large-scale genetic linkage analysis. The LA procedure has many advantages including the ability to multiplex signals under the same conditions, and lower cost since only one primer is needed.
Long-Term Persistence of Sterile Immunity in a Volunteer Immunized with X-Irradiated Plasmodium falciparum Sporozoites (1993)

Three volunteers were immunized by repeated exposure to the bites of Plasmodium falciparum-infected, X-irradiated mosquitoes to characterize immunologic responses and duration of protective immunity. A primary series of immunizations had been shown previously to induce sterile immunity in these volunteers against sporozoite-induced P. falciparum malaria. In the current study, antibodies to sporozoites circulated at high levels for at least 9-12 months after the volunteers were administered booster bites from X-irradiated infective mosquitoes. One volunteer challenged a second time with P. falciparum 9 months after his last immunization was again shown to be protected, whereas all 5 control subjects developed patent infections. These results set a new standard for persistence of sterile immunity against experimental P. falciparum infection.
The ontogeny of peroxisome-proliferator-activated receptor gene expression in the mouse and rat (1992)

The expression of the gene coding for peroxisome-proliferator-activated receptor (PPAR), a novel transacting factor belonging to the steroid superfamily, has been determined in the mouse and rat throughout development using hybridization histochemistry. Messenger RNA is demonstrable in the liver and brown fat from the fetal period onwards and, additionally, in the heart, kidney and gut post-natally. It is proposed that the upregulation of transcription of peroxisomal beta oxidation genes in specific tissues follows binding of the receptor to its natural ligand. Thus PPAR may have an important role in cold adaptation and non-shivering thermogenesis as well as in detoxification.
A Potential Virulence Gene,hylEfm, Predominates inEnterococcus faeciumof Clinical Origin (2003)

An open reading frame (hyl(Efm)) with homologies to previously described hyaluronidase genes has been identified in nonstool isolates of Enterococcus faecium. E. faecium isolates (n=577) from diverse sources were screened for the presence of hyl(Efm) and esp(Efm), a putative virulence gene associated with epidemic E. faecium strains. The presence of esp(Efm) was roughly twice that of hyl(Efm), but both were found primarily in vancomycin-resistant E. faecium isolates in nonstool cultures obtained from patients hospitalized in the United States. These data suggest that specific E. faecium strains may be enriched in determinants that make them more likely to cause clinical infections. Differences in the prevalence of these strains may help explain variations in the clinical importance of multiresistant E. faecium across different continents.
The Chromatographic Separation of Factor VIII on Aminohexyl Sepharose (1979)

A new method of factor VIII purification has been devised which involves chromatographic separation on aminohexyl-substituted agarose. Relatively large volumes of starting material can be processed compared to the volume of agarose employed and satisfactory yields are obtained. Factor-VIII-clotting activity is separated from the other related substances and resultant products appear to be stable. While separation of clotting activity occurs with relative ease, the antigen and ristocetin co-factor are hardly segregated, if at all. This provides a little more information about the interrelation of these substances. Results suggest that ion-exchange is involved in the mechanism of separation but additional hydrophobic or steric effects cannot be ruled out.
Exon/intron structure of the human alpha 3(IV) gene encompassing the Goodpasture antigen (alpha 3(IV)NC1). Identification of a potentially antigenic region at the triple helix/NC1 domain junction. (1992)

The Goodpasture antigen has been identified as the non-collagenous (NC1) domain of alpha 3(IV), a novel collagen IV chain (Saus, J., Wieslander, J., Langeveld, J., Quinones, S., and Hudson, B.G. (1988) J. Biol. Chem. 263, 13374-13380). In the present study, the exon/intron structure and sequence for 285 amino acids of human alpha 3(IV), comprising 53 amino acids of the triple-helical domain and the complete NC1 domain (232 amino acids), were determined. Based on the comparison of the amino acid sequences of the alpha 1(IV), alpha 2(IV), alpha 3(IV), and alpha 5(IV) NC1 domains, a phylogenetic tree was constructed which indicates that alpha 2(IV) was the first chain to evolve, followed by alpha 3(IV), and then by alpha 1(IV) and alpha 5(IV). The exon/intron structure of these domains is consistent with this evolution model. In addition, it appears that alpha 3(IV) changed most after diverging from the parental gene. Analysis of its primary structure reveals that, at the junction between the triple-helical and NC1 domains, there exists a previously unrecognized, highly hydrophilic region (GLKGKRGDSGSPATWTTR) which is unique to the human alpha 3(IV) chain, containing a cell adhesion motif (RGD) as an integral part of a sequence (KRGDSGSP) conforming to a number of protein kinase recognition sites. Based on primary structure data, we outline new aspects to be explored concerning the molecular basis of collagen IV function and Goodpasture syndrome.
Signal transducer and activator of transcription 3 is involved in cell growth and survival of human rhabdomyosarcoma and osteosarcoma cells (2007)

This study demonstrates that Stat3 phosphorylation is elevated in human rhabdomyosarcoma, osteosarcomas and soft-tissue sarcomas. Furthermore, the activated Stat3 pathway is important for cell growth and survival of human sarcoma cells.
Iraq War, 2004 (2014)

Research Article| September 01 2014 Iraq War, 2004 Hugh Martin Hugh Martin Search for other works by this author on: This Site Google Labor (2014) 11 (3): 5–12. https://doi.org/10.1215/15476715-2687705 Cite Icon Cite Share Icon Share Twitter Permissions Search Site Citation Hugh Martin; Iraq War, 2004. Labor 1 September 2014; 11 (3): 5–12. doi: https://doi.org/10.1215/15476715-2687705 Download citation file: Zotero Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search Books & JournalsAll JournalsLabor Search Advanced Search The text of this article is only available as a PDF. © 2014 by Labor and Working-Class History Association2014 Article PDF first page preview Close Modal Issue Section: The Common Verse You do not currently have access to this content.
Incorporating users location into snippet based Query recommendation system (2015)

As the access of web has been increased greatly for information retrieval and it is difficult to extract the relevant information in less time. Sometime Search engine does not able to understand user search intend behind query. Thus Query recommendation can be used to help user to state exactly their information need. Search engine can return appropriate result to meet users' information needs. There are various methods based on history of users and snippets to retrieve the information. But these methods fail to satisfy users need. Therefore in addition of history and snippets with users location information will produce better results. Snippet based method with incorporating users preferences and location results the URLs for given query, also it ranks the clicked URLs at the top of the result based on user profile. The performance of the system shows that the snippet based with incorporating users location give better and effective recommendation for all types queries as compared to previous methods including low frequency queries.
Frühgeburtlichkeit und fetaler Hirnschaden (2004)

Jahr für Jahr erleiden in Deutschland ca. 1000 frühgeborene Kinder einen Hirnschaden während der Perinatalzeit. Je nach Ausmaß und Lokalisation des Insultes entwickeln diese Kinder spastische Paresen, Choreoathetosen, Ataxien und sensomotorische Integrationsstörungen. Nicht selten sind auch Spätschäden im auditiven und visuellen System sowie Beeinträchtigungen der intellektuellen Leistungsfähigkeit zu beobachten. Wie neuere Untersuchungen zeigen, können diese Schäden wohl nicht nur durch einen Sauerstoffmangel verursacht werden, sondern auch im Zuge einer aszendierenden intrauterinen Infektion auftreten. So konnte nachgewiesen werden, dass mütterliches Fieber unter der Geburt oder eine erhöhte Zytokinkonzentration im Fruchtwasser mit einer erhöhten Inzidenz an kindlichen Hirnblutungen oder einer periventrikulären Leukomalazie assoziiert waren. Experimentell konnte gezeigt werden, dass Lipopolysacchride (LPS), Zellwandbestandteile gramnegativer Bakterien, eine prolongierte arterielle Hypotension beim Feten hervorrufen können. Diese Hypotension ist begleitet von einer plazentaren Minderperfusion und einem Abfall des zerebralen Sauerstofftransportes. Hirnschäden können in einer solchen Situation auftreten. Darüber hinaus gibt es deutliche Hinweise, dass eine Inflammation des fetalen Gehirns einen hypoxisch-ischämischen Insult verstärken kann. So konnte experimentell nachgewiesen werden, dass die Applikation von LPS in den Liquor cerebri zu signifikant größeren hypoxisch-ischämsichen Hirnschäden führte. Auch scheinen Zytokine, die im Rahmen einer aszendierenden intrauterinen Infektion freigesetzt werden, das fetale Nervenstützgewebe direkt zu schädigen. Betroffen sind die markscheidenbildenden Zellen, die sogenannten Oligodendrozyten, vor allem deren Vorläuferstufen. Zytokine induzieren hier einen apoptotischen Zelltod und beeinträchtigen die Ausdifferenzierung von Vorläuferzellen in reife Oligodendrozyten. Diese Schäden können durch die Gabe verschiedener Kortikosteroide signifikant reduziert werden. Angesichts der hohen Bedeutung von fetalen Hirnschäden für das weitere Leben der betroffenen Kinder, ist eine intensive Forschung zur Pathogenese dieser Insulte dringend geboten. Neue therapeutische Konzepte können nur auf dieser Basis entwickelt werden.
Differentiation and functional maturation of human cd14+ adherent peripheral blood monocytes by xenogeneic endothelial cells: up-regulation of costimulation, cytokine generation, and toll-like receptors1 (2002)

Porcine aortic endothelial cells can provide immunostimulatory signals to human peripheral blood adherent monocytes similar to allogeneic endothelial cells through the participation of innate immune mechanisms.
Identification of critical functional and regulatory domains in gelsolin. (1989)

Gelsolin can sever actin filaments, nucleate actin filament assembly, and cap the fast-growing end of actin filaments. These functions are activated by Ca2+ and inhibited by polyphosphoinositides (PPI). We report here studies designed to delineate critical domains within gelsolin by deletional mutagenesis, using COS cells to secrete truncated plasma gelsolin after DNA transfection. Deletion of 11% of gelsolin from the COOH terminus resulted in a major loss of its ability to promote the nucleation step in actin filament assembly, suggesting that a COOH-terminal domain is important in this function. In contrast, derivatives with deletion of 79% of the gelsolin sequence exhibited normal PPI-regulated actin filament-severing activity. Combined with previous results using proteolytic fragments, we deduce that an 11-amino acid sequence in the COOH terminus of the smallest severing gelsolin derivative identified here mediates PPI-regulated binding of gelsolin to the sides of actin filaments before severing. Deletion of only 3% of gelsolin at the COOH terminus, including a dicarboxylic acid sequence similar to that found on the NH2 terminus of actin, resulted in a loss of Ca2+-requirement for filament severing and monomer binding. Since these residues in actin have been implicated as potential binding sites for gelsolin, our results raise the possibility that the analogous sequence at the COOH terminus of gelsolin may act as a Ca2+-regulated pseudosubstrate. However, derivatives with deletion of 69-79% of the COOH-terminal residues of gelsolin exhibited normal Ca2+ regulation of severing activity, establishing the intrinsic Ca2+ regulation of the NH2-terminal region. One or both mechanisms of Ca2+ regulation may occur in members of the gelsolin family of actin-severing proteins.
Bone Morphogenetic Proteins: Neurotrophic Roles for Midbrain Dopaminergic Neurons and Implications of Astroglial Cells (1997)

Bone morphogenetic proteins (BMPs) are members of the transforming growth factor beta (TGF-beta) superfamily that have been implicated in tissue growth and remodelling. Recent evidence suggests that several BMPs are expressed in the developing and adult brain. Specifically, we show that BMP 2 and BMP 6 are expressed in the developing midbrain floor of the rat. We studied potential neurotrophic effects of BMPs on the in vitro survival, transmitter uptake and protection against MPP+ toxicity of mesencephalic dopaminergic neurons cultured from the embryonic midbrain floor at embryonic day (E) 14. At 10 ng/ml and under serum-free conditions, most BMPs promoted the survival of dopaminergic neurons visualized by tyrosine hydroxylase immunocytochemistry during an 8-day culture period, but to varying extents (relative potencies: BMP 6 = 12 > 2, 4, 7). BMPs 6 and 12 were as effective as fibroblast growth factor-2 (FGF-2) and glial cell line-derived neurotrophic factor, promoting survival 1.7-fold compared with controls. BMPs 9 and 11 were not effective. Dose-response curves revealed an EC50 for BMPs 2, 6 and 12 of 2 ng/ml. BMPs 2, 4, 6, 7, 9 and 12 also promoted DNA synthesis and astroglial cell differentiation, visualized by 5-bromodeoxyuridine (BrdU) incorporation and glial fibrillary acidic protein (GFAP) immunocytochemistry respectively. Suppression of cell proliferation and subsequent maturation of GFAP-positive cells by 5-fluorodeoxyuridine or aminoadipic acid abolished the neuron survival-promoting effect of BMP 2. This suggests that BMPs, like other non-TGF-beta factors affecting dopaminergic neuron survival, act indirectly, probably by stimulating the synthesis and/or release of glial-derived trophic factors. BMP 6 and BMP 7 also increased the uptake of [3H]dopamine without affecting the uptake of [3H]5-hydroxytryptamine and [3H]GABA, underscoring the specificity of the trophic effect. We conclude that several BMPs share a neurotrophic capacity for dopaminergic midbrain neurons with other members of the TGF-beta superfamily, but act indirectly, possibly through glial cells.
P wave dispersion is increased in pulmonary stenosis. (2006)

P wave dispersion and p max are increased in PS. While PWD was correlated with the pressure gradient that is the degree of narrowing, it was not correlated with the diameters of the right and left atria.
Nuclear Factor Kb: An Oxidative Stress-Responsive Transcription Factor of Eukaryotic Cells (A Review) (1992)

NF-kappa B is a multiprotein complex that can activate a great variety of genes involved in early defence reactions of higher organisms. In nonstimulated cells, NF-kappa B resides in the cytoplasm in an inactive complex with the inhibitor I kappa B. Pathogenic stimuli cause release of I kappa B and allow NF-kappa B to enter the nucleus, bind to DNA control elements and, thereby, induce the synthesis of mRNA. A puzzling feature of NF-kappa B is that its activation is triggered by a great variety of agents. These include the cytokines interleukin-1 and tumor necrosis factor, viruses, double-stranded RNA, endotoxins, phorbol esters, UV light and ionizing radiation. We recently found that also low concentrations of H2O2 activate NF-kappa B and that various antioxidants prevent the induction by H2O2. Subsequent analysis revealed that antioxidants not only suppress the activation of NF-kappa B by H2O2 but by all other inducers tested so far. In this review, we will discuss the evidences that NF-kappa B is an oxidative stress-responsive transcription factor of higher eukaryotic cells.
7MM traveling-wave Maser with gain bandwidth exceeding 200 MHz (1989)

The increase in sensitivity of radioreceiving systems for a millimeter wavelength band is a complex and important problem. It is of special significance for radioastronomical investigations where a low noise level of receiving devices plays a principal role both while the reception of regular and noise signals.
Loss of IGF2 Imprinting: A Potential Marker of Colorectal Cancer Risk (2003)

Loss of imprinting (LOI), an epigenetic alteration affecting the insulin-like growth factor II gene (IGF2), is found in normal colonic mucosa of about 30% of colorectal cancer (CRC) patients, but it is found in only 10% of healthy individuals. In a pilot study to investigate the utility of LOI as a marker of CRC risk, we evaluated 172 patients at a colonoscopy clinic. The adjusted odds ratio for LOI in lymphocytes was 5.15 for patients with a positive family history [95% confidence interval (95% CI), 1.70 to 16.96; probability P = 0.002], 3.46 for patients with adenomas (95% CI, 1.14 to 11.37; P = 0.026), and 21.7 for patients with CRC (95% CI, 3.48 to 153.6; P = 0.0005). LOI can be assayed with a DNA-based blood test, and it may be a valuable predictive marker of an individual's risk for CRC.
The Nucleolus Is the Site of Borna Disease Virus RNA Transcription and Replication (1998)

Borna disease virus (BDV) is a neurotropic nonsegmented negative-strand RNA virus with limited homology to rhabdoviruses and paramyxoviruses. A distinguishing feature of BDV is that it replicates in the nucleus of infected cells. Strand-specific probes used for in situ hybridization of infected rat brain showed that there was differential localization of positive- and negative-strand RNAs within the nucleus of neurons. Within nuclei, sense-strand RNAs were preferentially localized within nucleolar regions while genomic-sense RNAs were found in both nucleolar and nonnucleolar regions. These results suggested a role for the nucleolus in BDV replication. Nucleoli isolated from persistently infected neuroblastoma cells contained both genomic and antigenomic BDV RNA species as well as an enrichment of the 39/38-kDa and gp18 BDV proteins. Since the nucleolus is the site of rRNA transcription, we examined BDV transcription in the presence of inhibitors of RNA polymerase I. Inhibition of RNA polymerase I did not affect levels of BDV transcription.
Air drawing of polymers in the melt blowing nonwoven process: mathematical modelling (2004)

An air drawing model of polymers and a model of the air jet flow field in the melt blowing process are established. The flow field model is solved using the finite difference method. The numerical computation results of distributions of the air velocity and air temperature coincide with the experimental data. The air drawing model of polymers is solved with the aid of the distributions of the air velocity and air temperature. The predicted fibre diameter tallies well with the experimental data. It is found that a higher initial air velocity can produce finer fibres, while the effect of the initial air temperature is not significant. The results show great prospects for this research in the field of computer assisted design of melt blowing technology and equipment.
Regulation of Gli1 Localization by the cAMP/Protein Kinase A Signaling Axis through a Site Near the Nuclear Localization Signal (2005)

The hedgehog (Hh) pathway plays a critical role during development of embryos and cancer. Although the molecular basis by which protein kinase A (PKA) regulates the stability of hedgehog downstream transcription factor cubitus interruptus, the Drosophila homologue of vertebrate Gli molecules, is well documented, the mechanism by which PKA inhibits the functions of Gli molecules in vertebrates remains elusive. Here, we report that activation of PKA retains Gli1 in the cytoplasm. Conversely, inhibition of PKA activity promotes nuclear accumulation of Gli1. Mutation analysis identifies Thr374 as a major PKA site determining Gli1 protein localization. In the three-dimensional structure, Thr374 resides adjacent to the basic residue cluster of the nuclear localization signal (NLS). Phosphorylation of this Thr residue is predicted to alter the local charge and consequently the NLS function. Indeed, mutation of this residue to Asp (Gli1/T374D) results in more cytoplasmic Gli1 whereas a mutation to Lys (Gli1/T374K) leads to more nuclear Gli1. Disruption of the NLS causes Gli1/T374K to be more cytoplasmic. We find that the change of Gli1 localization is correlated with the change of its transcriptional activity. These data provide evidence to support a model that PKA regulates Gli1 localization and its transcriptional activity, in part, through modulating the NLS function.