gebhart/patrepeval huggingface dataset:
Manganese(III)-mediated carbon-carbon bond formation in the reaction of xanthenes with active methylene compounds (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTManganese(III)-mediated carbon-carbon bond formation in the reaction of xanthenes with active methylene compoundsHiroshi Nishino, Hironori Kamachi, Harumi Baba, and Kazu KurosawaCite this: J. Org. Chem. 1992, 57, 13, 3551–3557Publication Date (Print):June 1, 1992Publication History Published online1 May 2002Published inissue 1 June 1992https://pubs.acs.org/doi/10.1021/jo00039a010https://doi.org/10.1021/jo00039a010research-articleACS PublicationsRequest reuse permissionsArticle Views987Altmetric-Citations44LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
When was Dracula first translated into Romanian (2009)

Dracula is one of the world’s best-known books. The novel has never been out of print since its publication and has been translated into about 30 languages (Melton). Yet, paradoxically, one of the countries where it is least known is Romania. The usual explanation given for this situation is Romania’s recent history, particularly the period of Communist Party rule (1947-1989). Dracula, with its emphasis on vampires and the supernatural, was apparently regarded as an unsuitable or inappropriate novel in a state founded on the materialist and “scientific” principles of Marxism. Hence, no translation of Stoker’s novel was permitted during the Communist period, a fact noted by several contemporary commentators (for example, Mackenzie 20; Florescu and McNally, Prince 220). As a result, Romania was entirely unprepared for the explosion in the West of popular interest in Dracula and vampires during the 1970s. While increasing numbers of Western tourists visited Transylvania on their own searches for the literary and supernatural roots of Bram Stoker’s novel, they frequently returned disappointed since hardly anybody in Romania understood what they were searching for. For example, Romanian guides and interpreters working for the national tourist office were often bewildered when asked by Western tourists for more details about Dracula and vampires in Romania (Nicolae Păduraru, personal interview). It was not until after the fall of Nicolae Ceauşescu’s Communist regime that the first Romanian translation of Dracula was published in 1990, much to the surprise and puzzlement of many Romanians (Boia 226). This article closely examines the issue of when Dracula was first translated into Romanian. In particular, I question the frequently repeated claim that Stoker’s novel did not appear in Romanian until after the fall of Communism. Instead, I argue that there is strong evidence (albeit circumstantial) that some form of translation existed during the interwar period and, furthermore, that a full translation was prepared (even if never published) at some stage during the Communist period.
Co-Amorphization of Ibuprofen by Paracetamol for Improved Processability, Solubility, and In vitro Dissolution (2018)

Co-amorphous (COAM) systems of ibuprofen (IB) and paracetamol (PA), in clinical dose ratios, were prepared by ball milling to enhance solubility and dissolution of IB. Subsequently, COAM were characterized by solubility, processability, XRPD, DSC, ATR-FTIR, SEM, in-vitro dissolution and accelerated stability studies. Maximum increase in aqueous solubility of IB was seen in 500:200 mg dose ratio (COAM 1) with 6.7 fold rise from 78.3 ± 1.1 to 522.6 ± 1.29 ∆g/ml. COAM 1 exhibited 99.80 ± 0.58% dissolution of IB at 20 min in phosphate buffer, significantly high (P < 0.05) compared to plain IB. Thus saturation solubility and dissolution rate of IB was found significantly improved unlike PA. The flowability/ processability of COAM system was remarkably improved compared to pure IB, speculated due to as formation of miniscular forms of PA-IB, having strong adhesive interactions. XRPD and DSC results confirmed amorphization of IB. ATR-FTIR results evidenced hydrogen bonding interactions between both the drugs. In accelerated stability studies, flowability, XRPD, DSC and in-vitro dissolution studies demonstrated insignificant changes, thus confirming successful stabilisation of IB by PA.
The Potential of Human Allogeneic Juvenile Chondrocytes for Restoration of Articular Cartilage (2010)

Juvenile human chondrocytes have greater potential to restore articular cartilage than adult cells, and may be transplanted without the fear of rejection, suggesting a new allogeneic approach to restoring articular cartilage in older individuals.
Future of Fat as Raw Material for Tissue Regeneration (2003)

Tissue replacement traditionally requires use of autologous tissue and is associated with the attendant morbidity of donor site harvest. In the case of allograft transplantation, there are concerns, similar to those associated with organ transplantation, of rejection and immunosuppression. For these reasons, emphasis has been placed on the development of tissue-engineered substitutes that incorporate autologous stem cells into tissue-engineered scaffolds. The authors' laboratory has characterized a population of cells obtained from processed lipoaspirate (PLA), which have the capacity in vitro to differentiate into osteoblasts, chondrocytes, myocytes, adipocytes, and neuron-like cells. Adipose tissue is an abundant, expendable, and easily obtained tissue that may prove to be an ideal source of autologous stem cells for engineering tissues.
Function of the LFA‐1 and T4 molecules in the direct activation of resting human B lymphocytes by T lymphocytes (1986)

Activated T lymphocytes can provide all of the signals necessary to induce the proliferation of resting B lymphocytes. The activation signal is presumably initiated through direct T-B lymphocyte contact. The role of the leukocyte function antigen-1 (LFA-1) and T4 molecules in the activation of purified, small B lymphocytes by mitomycin C-treated T lymphocytes was examined by using monoclonal antibodies that react with and inhibit the function of these molecules. Anti-LFA-1 antibody binding significantly inhibited T-B lymphocyte interactions that result in B lymphocyte proliferation. In contrast, the presence of anti-T4 antibodies at concentrations as high as 100 micrograms/ml did not inhibit this interaction. These results indicate that the B lymphocyte activation signal may not be mediated through the interaction of T4 molecules with major histocompatibility complex class II antigens of the B lymphocyte but is a cell-cell contact-dependent event that is facilitated by LFA-1 molecules.
Neuroprotection mediated by inhibition of calpain during acute viral encephalitis (2016)

Neurologic complications associated with viral encephalitis, including seizures and cognitive impairment, are a global health issue, especially in children. We previously showed that hippocampal injury during acute picornavirus infection in mice is associated with calpain activation and is the result of neuronal death triggered by brain-infiltrating inflammatory monocytes. We therefore hypothesized that treatment with a calpain inhibitor would protect neurons from immune-mediated bystander injury. C57BL/6J mice infected with the Daniel's strain of Theiler's murine encephalomyelitis virus were treated with the FDA-approved drug ritonavir using a dosing regimen that resulted in plasma concentrations within the therapeutic range for calpain inhibition. Ritonavir treatment significantly reduced calpain activity in the hippocampus, protected hippocampal neurons from death, preserved cognitive performance, and suppressed seizure escalation, even when therapy was initiated 36 hours after disease onset. Calpain inhibition by ritonavir may be a powerful tool for preserving neurons and cognitive function and preventing neural circuit dysregulation in humans with neuroinflammatory disorders.
Emerging roles of the intestine in control of cholesterol metabolism (2006)

The liver is considered the major "control center" for maintenance of whole body cholesterol homeostasis. This organ is the main site for de novo cholesterol synthesis, clears cholesterol-containing chylomicron remnants and low density lipoprotein particles from plasma and is the major contributor to high density lipoprotein (HDL; good cholesterol) formation. The liver has a central position in the classical definition of the reverse cholesterol transport pathway by taking up periphery-derived cholesterol from lipoprotein particles followed by conversion into bile acids or its direct secretion into bile for eventual removal via the feces. During the past couple of years, however, an additional important role of the intestine in maintenance of cholesterol homeostasis and regulation of plasma cholesterol levels has become apparent. Firstly, molecular mechanisms of cholesterol absorption have been elucidated and novel pharmacological compounds have been identified that interfere with the process and positively impact plasma cholesterol levels. Secondly, it is now evident that the intestine itself contributes to fecal neutral sterol loss as a cholesterol-secreting organ. Finally, very recent work has unequivocally demonstrated that the intestine contributes significantly to plasma HDL cholesterol levels. Thus, the intestine is a potential target for novel anti-atherosclerotic treatment strategies that, in addition to interference with cholesterol absorption, modulate direct cholesterol excretion and plasma HDL cholesterol levels.
Leflunomide analogue FK778 is vasculoprotective independent of its immunosuppressive effect: potential applications for restenosis and chronic rejection (2003)

Our results demonstrate that FK778 inhibits neointima formation by way of a mechanism that is independent of DHODH inhibitory activity on vascular smooth muscle cell. Interestingly, the effect of imatinib was inhibited by uridine, suggesting that part of its action on vascular stenosis could be mediated through inhibition of pyrimidine synthesis.
A pea antisense gene for the chloroplast stromal processing peptidase yields seedling lethals in Arabidopsis: survivors show defective GFP import in vivo (2003)

The stromal processing peptidase (SPP) of chloroplasts is a metalloendopeptidase that cleaves in vitro a broad range of precursor substrates. Here, we have investigated SPP's role in vivo. Two pea cDNA antisense constructs encoding either full-length SPP (AS4.0) or its N-terminal half (AS2.2) are introduced into Arabidopsis, which contains one gene for SPP that codes for one isoform. Our analyses show that AS4.0 produces a strong mutant phenotype, with a large percentage of the plants dying as seedling lethals. Surviving plants exhibited slower shoot and root growth, and grossly aberrant leaf morphology. Green and white sectoring, and purple pigmentation was observed. In cells where chloroplasts could be identified, they were fewer in number by at least 40%, thylakoids were not fully developed, and starch granules accumulated. The phenotype produced by AS2.2 was less severe. Using green fluorescent protein (GFP) fused to a transit peptide as a reporter, we examined import into chloroplasts in vivo. In the Arabidopsis antisense lines, GFP was located primarily in the cytosol, indicating that an early step in the import pathway was impeded. In a tobacco AS14 line expressing AS2.2, GFP was located in the cytosol, on the envelope, and in the stroma. The three patterns were observed in different cells, suggesting that the import capacity of individual cells was not the same. Our in vivo studies demonstrate that SPP is essential for chloroplast biogenesis and plant survival. SPP does not act independently in the stroma, but its activity influences earlier steps in the import pathway.
Formation velocity and density; the diagnostic basics for stratigraphic traps (1974)

Abstract A multiplicity of factors influence seismic reflection coefficients and the observed gravity of typical sedimentary rocks. Rock velocity and density depend upon the mineral composition and the granular nature of the rock matrix, cementation, porosity, fluid content, and environmental pressure. Depth of burial and geologic age also have an effect.Lithology and porosity can be related empirically to velocity by the time-average equation. This equation is most reliable when the rock is under substantial pressure, is saturated with brine, and contains well-cemented grains. For very low porosity rocks under large pressures, the mineral composition can be related to velocity by the theories of Voigt and Reuss.One effect of pressure variation on velocity results from the opening or closing of microcracks. For porous sedimentary rocks, only the difference between overburden and fluid pressure affects the microcrack system. Existing theory does not take into account the effect of microcrack closure on the elastic behavior of rocks under pressure or the chemical interaction between water and clay particles.The theory of Gassmann can be used to calculate the effect of different saturating fluids on the P-wave velocity of porous rocks. The effect may be large enough in shallow, recent sediments to permit gas sands to be distinguished from water sands on seismic records. At depths greater than about 6000 ft, however, the reflection coefficient becomes essentially independent of the nature of the fluid.Data show the systematic relationship between velocity and density in sedimentary rocks. As a result, reflection coefficients can often be estimated satisfactorily from velocity information alone.
Dual roles of the chemorepellent axon guidance molecule RGMa in establishing pioneering axon tracts and neural fate decisions in embryonic vertebrate forebrain (2012)

Repulsive guidance molecule A (RGMa) is a glycosylphosphatidylinositol-anchored plasma membrane protein that was originally identified based on its chemorepulsive activity during axon navigation in the developing nervous system. Knock down of RGMa has previously shown to perturb axon navigation in the developing Xenopus forebrain (Wilson and Key, 2006). In order to further understand the in vivo role of RGMa in axon guidance, we have adopted an in vivo gain-of-function approach. RGMa was mosaically overexpressed in the developing Xenopus embryo by the injection of mRNA into single blastomeres. Ectopic expression of RGMa affected the morphology and the topography of developing axon tracts in vivo. Pioneer axons misrouted or aberrantly projected in response to ectopic RGMa in the developing Xenopus forebrain, confirming the in vivo chemorepulsive activity of this ligand. In addition, we show here for the first time that overexpression of RGMa acts cell-autonomously to generate ectopic neurons in the developing embryonic brain. Taken together, the current study reveals a pleiotropic role of RGMa in early vertebrate embryonic brain in the spatial organization of axon tracts, pioneer axon guidance, and neural cell differentiation.
The use of extended Boolean logic in information retrieval (1984)

An extended Boolean retrieval strategy has previously been introduced in which the individual Boolean operators can be treated more or less strictly, depending on the perceived strength of association of the query terms. The extended Boolean system is illustrated by examples and evaluation output is used to demonstrate the effectiveness of the operations.
Exploration of Orally Available Calpain Inhibitors 2:  Peptidyl Hemiacetal Derivatives (2006)

We previously reported a potent calpain inhibitor 1 (SJA6017, N-(4-fluorophenyl)-l-valyl-l-leucinal), which displayed relatively low oral bioavailability (BA). Replacing the metabolically labile aldehyde moiety of 1with more chemically stable warheads, such as a cyclic hemiacetal, hydrazone, and alpha-ketoamide, provided the inhibitors with improved in vitro metabolic stability. Cyclic hemiacetal 2 was the most stable of these compounds. The optimization of 2 led to hemiacetal 8 (SNJ-1715) which exhibited high potency, good aqueous solubility, excellent oral BA, and prolonged plasma half-life in rats. Furthermore, 8 showed neuroprotective efficacy via oral administration in a rat retinal ischemia model.
Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda. (1989)

We constructed a derivative of transposon Tn5 called Tn5supF for insertion mutagenesis and sequencing DNAs cloned in phage lambda. This element carries a supF amber-suppressor tRNA gene. Its insertion into lambda can be selected by plaque formation by using nonsuppressing (sup0) Escherichia coli for amber mutant lambda phage and sup0 dnaB-amber E. coli for nonamber lambda phage. Tn5supF is just 264 base pairs long. It transposes efficiently and inserts quasi-randomly into DNA targets. The unique sequences near its termini can be used as primer binding sites for dideoxynucleotide DNA sequencing, thus permitting the direct sequencing of DNAs cloned in phage lambda without subcloning.
The rGel/BLyS fusion toxin specifically targets malignant B cells expressing the BLyS receptors BAFF-R, TACI, and BCMA (2007)

B lymphocyte stimulator (BLyS) is crucial for B-cell survival, and the biological effects of BLyS are mediated by three cell surface receptors designated B cell-activating factor receptor (BAFF-R), transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), and B-cell maturation antibody (BCMA). Increased expression of BLyS and its receptors has been identified in numerous B-cell malignancies. We generated a fusion toxin designated rGel/BLyS for receptor-mediated delivery of the recombinant gelonin (rGel) toxin to neoplastic B cells, and we characterized its activity against various B-cell tumor lines. Three mantle cell lymphoma (MCL) cell lines (JeKo-1, Mino, and SP53) and two diffuse large B-cell lymphoma (DLBCL) cell lines (SUDHL-6 and OCI-Ly3) expressing all three distinct BLyS receptors were found to be the most sensitive to the fusion toxin (IC(50) = 2-5 pmol/L and 0.001-5 nmol/L for MCL and DLBCL, respectively). The rGel/BLyS fusion toxin showed specific binding to cells expressing BLyS receptors and rapid internalization of the rGel component into target cells. The cytotoxic effects of rGel/BLyS were inhibited by pretreatment with free BLyS or with soluble BAFF-R, TACI, and BCMA decoy receptors. This suggests that the cytotoxic effects of the fusion toxin are mediated through BLyS receptors. The rGel/BLyS fusion toxin inhibited MCL cell growth through induction of apoptosis associated with caspase-3 activation and poly (ADP-ribose) polymerase cleavage. Our results suggest that BLyS has the potential to serve as an excellent targeting ligand for the specific delivery of cytotoxic molecules to neoplastic B cells expressing the BLyS receptors, and that the rGel/BLyS fusion toxin may be an excellent candidate for the treatment of B-cell malignancies especially MCL and DLBCL.
The Role of CC Chemokine Receptor 5 (CCR5) in Islet Allograft Rejection (2002)

Chemokines are important regulators in the development, differentiation, and anatomic location of leukocytes. CC chemokine receptor 5 (CCR5) is expressed preferentially by CD4+ T helper 1 (Th1) cells. We sought to determine the role of CCR5 in islet allograft rejection in a streptozotocin-induced diabetic mouse model. BALB/c islet allografts transplanted into CCR5−/− (C57BL/6) recipients survived significantly longer (mean survival time, 38 ± 8 days) compared with those transplanted into wild-type control mice (10 ± 2 days; P &amp;lt; 0.0001). Twenty percent of islet allografts in CCR5−/− animals without other treatment survived &amp;gt;90 days. In CCR5−/− mice, intragraft mRNA expression of interleukin-4 and -5 was increased, whereas that of interferon-γ was decreased, corresponding to a Th2 pattern of T-cell activation in the target tissues compared with a Th1 pattern observed in controls. A similar Th2 response pattern was also observed in the periphery (splenocytes responding to donor cells) by enzyme-linked immunosorbent spot assay. We conclude that CCR5 plays an important role in orchestrating the Th1 immune response leading to islet allograft rejection. Targeting this chemokine receptor, therefore, may provide a clinically useful strategy to prevent islet allograft rejection.
Diagnosis of Porcine Reproductive and Respiratory Syndrome (1995)

The most suitable tissue samples and test procedures for the etiologic diagnosis of porcine reproductive and respiratory syndrome (PRRS) were found to depend on several variables including the age of the pig from which tissues were collected, the stage of infection (acute or persistent), the available complement of diagnostic reagents, and the urgency in obtaining results. When the diagnosis involved acute infection of congenitally or neonatally infected pigs, and susceptible cell culture(s) was available for virus isolation, then both serum and alveolar macrophages (AM) were reliable samples. Alveolar macrophages flushed from infected lungs provided a temporal advantage, however, in that in addition to their use for virus isolation, i.e., from a lysate of AM, they could be cultured in vitro and examined for the presence of viral antigens by immunofluorescence microscopy (FA) as early as 1 hour after they were added to the culture vessel. The examination of AM in this manner also circumvented the need for additional cell cultures to test for infectious virus. Testing presuckling sera by indirect FA for antibodies to PRRS virus also was of diagnostic value and, like FA with AM, could be completed soon after sample collection. For older pigs, AM were more reliable than serum, lungs, or any of 27 other tissues evaluated as diagnostic samples and were often the only samples in which infectious virus and viral antigens were detected when pigs were euthanized more than 3 weeks postexposure. A simple procedure for on-farm collection of AM as well as methods for testing AM for viral antigens and neonatal (presuckling) sera for homologous antibody in a modestly equipped laboratory, such as one that might be maintained by a veterinary practitioner, are described and discussed.
Detection and quantification of 3,5,3′-triiodothyronine and 3,3′,5′-triiodothyronine by electrospray ionization tandem mass spectrometry (2005)

A novel and rapid method for identifying and quantifying 3,5,3'-triiodothyronine (T3) and 3,3',5'-triiodothyronine (rT3; reverse T3) has been introduced using electrospray ionization tandem mass spectrometry (ESI-MS/MS). MS(2) spectra in either negative ionization mode or positive ionization mode can be used to differentiate T3 and rT3. Quantification of the T3 and rT3 isomers under the negative ionization mode is also achieved without prior separation by HPLC.