gebhart/patrepeval huggingface dataset:
PREVENTION OF EXCESS HEMOLYSIS DURING CARDIOPULMONARY BYPASS BY THE USE OF PLURONIC F-68 (1973)

Subacute toxicity of Pluronic F-68, a non-ionic surfactant, was investigated with Wister rats.Three times every other day intraperitoneal injections of Pluronic F-68 at a dose of 1.0, 4.0, 10 or 40 mg/ml produced no gross changes in the kidney and liver.But the fatty granulas were observed in the renal tubules and in liver.In 13 open heart surgery 0.6-1.0 mg/ml of Pluronic F-68 were administered in bypass circuit. Pluronic F-68 was very effective to prevent the hemolysis and no significant harmful side effects were recognized in our series.But special care have to be directed in prolonged extra-corporeal circulation.
Enzymatic hydrolysis of (±)-trans-1,2-diacetozycycloalkanes. A facile route to optically-active cycloalkane-1,2-diols (1986)

D. H. G. Crout, V. S. B. Gaudet, K. Laumen and M. P. Schneider, J. Chem. Soc., Chem. Commun., 1986, 808 DOI: 10.1039/C39860000808
Calorie restriction up-regulates the plasma membrane redox system in brain cells and suppresses oxidative stress during aging (2006)

The plasma membrane (PM) contains redox enzymes that provide electrons for energy metabolism and recycling of antioxidants such as coenzyme Q and alpha-tocopherol. Brain aging and neurodegenerative disorders involve impaired energy metabolism and oxidative damage, but the involvement of the PM redox system (PMRS) in these processes is unknown. Caloric restriction (CR), a manipulation that protects the brain against aging and disease, increased activities of PMRS enzymes (NADH-ascorbate free radical reductase, NADH-quinone oxidoreductase 1, NADH-ferrocyanide reductase, NADH-coenzyme Q10 reductase, and NADH-cytochrome c reductase) and antioxidant levels (alpha-tocopherol and coenzyme Q10) in brain PM during aging. Age-related increases in PM lipid peroxidation, protein carbonyls, and nitrotyrosine were attenuated by CR, levels of PMRS enzyme activities were higher, and markers of oxidative stress were lower in cultured neuronal cells treated with CR serum compared with those treated with ad libitum serum. These findings suggest important roles for the PMRS in protecting brain cells against age-related increases in oxidative and metabolic stress.
In vivo Stability of Poly(ethylene glycol)-Collagen Composites (1997)

Collagen materials were crosslinked by two different difunctional poly ethylene glycols (PEGs) for a variety of applications. Crosslinking was mediated by carbamation of primary amines on collagen with N-hydroxysuccinimide ester end-groups on the PEG crosslinkers. One crosslinker, disuccinimidyl glutarate PEG (di-SG-PEG), contained internal ester linkages which were capable of hydrolyzing and hence produced degradable networks. The other crosslinker, disuccinimidyl propionate PEG (di-SE-PEG), contained hydrolytically stable internal ether linkages and hence produced a more durable network. In vitro experiments verified that di-SE-PEG networks were more hydrolytically stable. In vivo experiments (rat subcutaneous model) revealed similar degradation rates for both networks indicating that other degradation mechanisms (probably enzymatic) also influence degradation in vivo. Histologic analysis revealed a mild inflammatory response to the implants accompanied by fibroblast ingrowth. A second in vivo study (bovine collagen hypersensitized guinea pigs), examined the immunogenicity of di-SG-PEG crosslinked materials and revealed that the PEG crosslinked implants showed lower antibody titers compared to non PEG-crosslinked controls. Another in vivo study compared the osteoconductive properties of di-SG-PEG crosslinked fibrillar collagen with uncrosslinked fibrillar collagen in a rat parietal defect model. Both of the collagen implant materials displayed elevated levels of new bone formation relative the non implanted sites with the PEG crosslinked implant displaying a slightly higher density of new bone forming cells. In an ophthalmic application, methylated collagen was crosslinked by either di-SG- PEG or di-SE-PEG crosslinkers to form clear lenticules. In de-epithelialized primate corneas, di-SG-PEG and crosslinked implants resorbed within 3 months whereas the di-SE-PEG crosslinked lenticules exhibited enhanced stability.
Angiotensin II is associated with activation of NF-κB-mediated genes and downregulation of PPARs (2002)

Angiotensin II (ANG II) promotes vascular inflammation through nuclear factor-kappaB (NF-kappaB)-mediated induction of pro-inflammatory genes. The role of peroxisome proliferator-activated receptors (PPARs) in modulating vascular inflammation and atherosclerosis in vivo is unclear. The aim of the present study was to examine the effects of ANG II on PPARs and NF-kappaB-dependent pro-inflammatory genes in the vascular wall in an in vivo model of atherosclerosis and aneurysm formation. Six-month-old male apolipoprotein E-deficient (apoE-KO) mice were treated with ANG II (1.44 mg/kg per day for 30 days). ANG II enhanced vascular inflammation, accelerated atherosclerosis, and induced formation of abdominal aortic aneurysms. These effects of ANG II in the aorta were associated with downregulation of both PPAR-alpha and PPAR-gamma mRNA and protein and an increase in transcription of monocyte chemotactic protein-1 (MCP-1), macrophage-colony stimulating factor (M-CSF), endothelial-selectin (E-selectin), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) throughout the entire aorta. ANG II also activated NF-kappaB with increases in both p52 and p65 NF-kappaB subunits. In summary, these in vivo results indicate that ANG II, through activation of NF-kappaB-mediated pro-inflammatory genes, promotes vascular inflammation, leading to acceleration of atherosclerosis and induction of aneurysm in apoE-KO mice. Downregulation of PPAR-alpha and -gamma by ANG II may diminish the anti-inflammatory potential of PPARs, thus contributing to enhanced vascular inflammation.
New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugatesFu-Tong Liu, Mark Zinnecker, Toshiyuki Hamaoka, and David H. KatzCite this: Biochemistry 1979, 18, 4, 690–697Publication Date (Print):February 20, 1979Publication History Published online1 May 2002Published inissue 20 February 1979https://pubs.acs.org/doi/10.1021/bi00571a022https://doi.org/10.1021/bi00571a022research-articleACS PublicationsRequest reuse permissionsArticle Views405Altmetric-Citations343LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Simple Molybdenum(IV) Olefin Complexes of the Type Mo(NR)(X)(Y)(olefin) (2010)

Exposure of heptane solutions of Mo(NAr)(CHCMe2Ph)(Me2Pyr)(OAr) (1a; Ar = 2,6-diisopropylphenyl), Mo(NAr)(CHCMe3)(Me2Pyr)[OCMe(CF3)2] (1b), and Mo(NAr)(CHCMe2Ph)(Me2Pyr)(OSiPh3) (1c) to one atmosphere of ethylene for 12 h yields the ethylene complexes, Mo(NAr)(CH2CH2)(Me2Pyr)(OAr) (2a), Mo(NAr)(CH2CH2)(Me2Pyr)[OCMe(CF3)2] (2b), and Mo(NAr)(CH2CH2)(Me2Pyr)(OSiPh3) (2c). Addition of 1 equiv of triphenylsilanol to a solution of 2c gives Mo(NAr)(CH2CH2)(OSiPh3)2 (3) readily. Mo(NAr)(CHCMe2Ph)(OTf)2(dme) reacts slowly with ethylene (60 psi) in toluene at 80 °C to give cis and trans isomers of Mo(NAr)(CH2CH2)(OTf)2(dme) (4a) in the ratio of ∼2(cis):1. Addition of lithium 2,5-dimethylpyrrolide to 4a under 1 atm of ethylene produces Mo(NAr)(CH2CH2)(η1-Me2Pyr)(η5-Me2Pyr) (5a). Mo(NAr)(CHCMe2Ph)(η1-MesPyr)2 (MesPyr = 2-mesitylpyrrolide) reacts cleanly with ethylene in benzene at 60 °C over a period of four days to give exclusively Mo(NAr)(CH2CH2)(MesPyr)2 (5b). Treatment of 5b with 2 equiv of (CF3)2CHOH in ether yields Mo(NAr)(CH2CH2)[OCH(CF3)2]2(Et2O) (6). Neat styrene reacts with 2c and 3 to generate the styrene complexes, Mo(NAr)(CH2CHPh)(Me2Pyr)(OSiPh3) (7) and Mo(NAr)(CH2CHPh)(OSiPh3)2 (8), respectively. Similarly, the trans-3-hexene complex, Mo(NAr)(trans-3-hexene)(OSiPh3)2 (9a), can be prepared from 3 and neat trans-3-hexene. When 3 is exposed to 1 atm of ethylene, the molybdacyclopentane species, Mo(NAr)(C4H8)(OSiPh3)2 (10), is generated. X-ray structural studies were carried out on 2c, 5a, 6, 8, 9a, and 10. All evidence suggests that alkene exchange at the Mo(IV) center is facile, followed by cis,trans isomerization and isomerization via double bond migration. In addition, trace amounts of alkylidene complexes are formed that result in slow metathesis reactions of free olefins to give (e.g.) a distribution of all possible linear olefins from an initial olefin and its double bond isomers.
Blockade of the Extracellular Signal-regulated Kinase Pathway Induces Marked G1 Cell Cycle Arrest and Apoptosis in Tumor Cells in Which the Pathway Is Constitutively Activated (2001)

Constitutive activation of the ERK pathway is associated with the neoplastic phenotype of a relatively large number of human tumor cells. Blockade of the ERK pathway by treatment with PD98059, a specific inhibitor of mitogen-activated protein (MAP) kinase/ERK kinase (MEK), completely suppressed the growth of tumor cells in which the pathway is constitutively activated (RPMI-SE and HT1080 cells). Consistent with its prominent antiproliferative effect, PD98059 induced a remarkable G(1) cell cycle arrest, followed by a modest apoptotic response, in these tumor cells. Selective up-regulation of p27(Kip1) was observed after PD98059 treatment of RPMI-SE and HT1080 cells. Overexpression in RPMI-SE cells of either a kinase-negative form of MEK1 or wild-type MAP kinase phosphatase-3 also induced up-regulation of p27(Kip1). The up-regulation of p27(Kip1) correlated with increased association of p27(Kip1) with cyclin E-cyclin-dependent kinase (CDK) 2 complexes, a concomitant inhibition of cyclin E-CDK2 kinase activity, and a consequent decrease in the phosphorylation state of retinoblastoma protein, which would culminate in the marked G(1) cell cycle arrest observed in these tumor cells. These results suggest that the complete growth suppression that follows specific blockade of the ERK pathway in tumor cells in which the pathway is constitutively activated is mediated by up-regulation of p27(Kip1).
The Signal Detection and Control Circuit Design for Confocal Auto-Focus System (2016)

Based on the demands of Confocal Auto-Focus system, the implementation method of signal measurement circuit and control circuit is given. Using the high performance instrumental amplifier AD620BN, low noise precision FET Op amplifier AD795JRZ and ultralow offset voltage Op amplifier OP07EP, a signal measurement circuit used to converse the two differential light intensity signal to electric signal is designed. And a control circuit which takes MCU MSP430F149 as core processes the former signal and generate a control signal driving the platform for auto-focusing. The experimental results proved the feasibility and correctness of circuits. And the system meets the design requirement.
Personalised image-based templates for intra-operative guidance (2005)

The high rate of unplanned perforation, poor fixation, and nerve injury with freehand pedicle screw insertion has led to the use of image-guided navigation systems. Although these improve accuracy, they have several drawbacks that could be overcome by using image-based drilling guide templates. The accuracy of such templates was tested in a cadaveric study of screw placement in the lumbar, thoracic, and cervical regions of the spine. The dimensional stability with autoclaving of duraform polyamide, to be used for manufacturing the guides, was first determined using test specimens. Computed tomography (CT) images were acquired of 4 cadaveric spines, and placement of 4 cervical, 32 thoracic, and 14 lumbar screws was planned. Eighteen personalized drilling guide templates, in four different designs, were built. Orthopaedic surgeons experienced in the freehand techniques used the templates. CT images were acquired to assess placement position with respect to the pedicle. Duraform polyamide was found to be unaffected by sterilization. Two of the template designs facilitated the placement of 20/20 screws without error. Templates can lead to successful screw placement, even in small pedicles, providing their design is optimized for the application area, e.g. with enhanced rotational stabilization.
Site 1 sodium channel blockers prolong the duration of sciatic nerve blockade from tricyclic antidepressants (2004)

Many recent reports in the literature address the local anesthetics efficacy of tricyclic antidepressants (TCAs). Here we investigated whether nerve block from TCAs is prolonged by site 1 sodium channel blockers such as tetrodotoxin and saxitoxin, which are known to prolong block from conventional local anesthetics. Tetrodotoxin and saxitoxin greatly prolonged block from TCAs. For example, the median duration of thermal nociceptive blocks for 10 mM amitriptyline, nortriptyline and doxepin were 0, 0, and 124 min; co-injection with 20 microM TTX (median block duration=0), yielded blocks lasting 404, 325, and 697 min, respectively. Co-injection of 12 microM saxitoxin (median block duration=0) with 10 mM amitriptyline resulted in a thermal nociceptive block duration of 373 min. Co-injection of 7.7 mM bupivacaine and 7.7 mM amiptriptyline did not result in block prolongation. Systemic (subcutaneous) delivery of tetrodotoxin or amitriptyline did not result in prolongation of block from the other class of drug injected at the sciatic nerve. In TCA-containing formulations, motor blockade was consistently longer than thermal nociceptive block; motor blockade was also prolonged by tetrodotoxin and saxitoxin. In summary site 1 sodium channel blockers prolong the duration of TCAs via a locally mediated mechanism.
Electrochemical Delamination of CVD-Grown Graphene Film: Toward the Recyclable Use of Copper Catalyst (2011)

The separation of chemical vapor deposited (CVD) graphene from the metallic catalyst it is grown on, followed by a subsequent transfer to a dielectric substrate, is currently the adopted method for device fabrication. Most transfer techniques use a chemical etching method to dissolve the metal catalysts, thus imposing high material cost in large-scale fabrication. Here, we demonstrate a highly efficient, nondestructive electrochemical route for the delamination of CVD graphene film from metal surfaces. The electrochemically delaminated graphene films are continuous over 95% of the surface and exhibit increasingly better electronic quality after several growth cycles on the reused copper catalyst, due to the suppression of quasi-periodical nanoripples induced by copper step edges. The electrochemical delamination process affords the advantages of high efficiency, low-cost recyclability, and minimal use of etching chemicals.
A Novel, Testis-Specific Member of the Cellular Lipophilic Transport Protein Superfamily, Deduced from a Complimentary Deoxyribonucleic Acid Clone1 (1994)

A novel member of the cellular lipophilic transport protein superfamily was identified after an antiserum raised against cellular retinoic acid-binding protein (CRABP) was found also to contain antibodies against another 15-kDa protein present in the cytosol of pubertal and adult rat testis. These antibodies were used to screen a rat testis cDNA expression library and isolate a 561-bp clone containing a full open reading frame from which the sequence of a novel 132 amino acid protein was deduced. The protein has 58% amino acid sequence identity to bovine myelin P2, 58% identity to murine adipocyte lipid-binding protein, and 40% identity to rat CRABP. Although the endogenous ligand has not yet been identified, conservation of residues involved in the binding of carboxylate groups suggests that the ligand is a fatty acid or an acidic retinoid. Tissue-specific expression was examined by Northern analysis and immunolocalization and appears to be restricted to late germ cells within the testis and epididymis. Immunostaining was first detectable in mid-pachytene spermatocytes and increased in intensity as these cells progressed to elongated spermatids, suggesting that this testis lipid-binding protein has a specific role in sperm development.
A coupled-cavity monolithic optical frequency comb generator (1996)

We have designed a monolithic optical frequency comb generator (M-OFC-G) that uses an external coupled cavity for the 1.5-μm region. The comb envelope was observed with a span or 6.3 THz and the total cavity transmission has been 16%. With an additional etalon inside the external coupled cavity, the comb envelope spanned 7 THz while the transmission dropped to 9%. We also demonstrated the possibility of increasing the power of selected sidebands by using a coupled cavity at the output port of the M-OFC-G. We observed an increase of the power of the selected sidebands within a range of 5.6 THz.
Evans Blue Dye as an in vivo marker of myofibre damage: optimising parameters for detecting initial myofibre membrane permeability (2002)

Evans Blue Dye (EBD) is widely used to study cellular membrane permeability and has recently been utilised in mdx mice to identify permeable skeletal myofibres that have become damaged as a result of muscular dystrophy. EBD has the potential to be a useful vital stain of myofibre permeability in other models of skeletal muscle injury and membrane-associated fragility. The parameters for its use for such purposes were optimised in the present study, of particular interest is the use of EBD to identify the onset of muscle damage. This study compared intravenous vs. intraperitoneal injection; tissue fixation; volume of EBD; time of availability in tissue; and persistence after injection in mdx mice (with endogenous muscle damage) and control mice. Satisfactory labelling of permeable myofibres was seen in frozen sections viewed with fluorescence microscopy when intraperitoneal injection of a 1% EBD solution injected at 1% volume relative to body mass was administered between 16 and 24 h prior to tissue sampling. EBD labelling was then assessed in three mouse models of experimental injury and repair-cut injury, whole muscle grafts, and exercise-induced muscle damage. These experiments demonstrated that (i) following a cut injury across myofibres, EBD penetrated up to 150 microm from the injury site over a 20-h period; (ii) EBD was present throughout myofibres of avascular whole muscle graft by one day after transplantation; and (iii) damaged myofibres were detected within 20 min after controlled lengthening-contraction exercise. This simple and inexpensive technique has sensitivity for the detection of increased myofibre permeability and/or sublethal damage that has advantages over other traditional histological techniques at the light microscopy level.
Necessary and sufficient conditions for hyperplane transversals (1989)

We will prove that a finite family B = {B1, B2, …, Bn} of connected compact sets in Rd has a hyperplane transversal if and only if for some k there exists a set of points P = {P1, P2, …, Pn} (i.e. a k-dimensional labeling of the family) which spans Rk and every k + 2 sets of B are met by a k-flat consistent with the order type of P. This is a common generalization of theorems of Hadwiger, Katchalski, Goodman-Pollack and Wenger.
Hypervariability of simple sequences as a general source for polymorphic DNA markers (1989)

Short simple sequence stretches occur as highly repetitive elements in all eukaryotic genomes and partially also in prokaryotes and eubacteria. They are thought to arise by slippage like events working on randomly occurring internally repetitive sequence stretches. This predicts that they should be generally hypervariable in length. I have used the polymerase chain reaction (PCR) process to show that several randomly chosen simple sequence loci with different nucleotide composition and from different species show extensive length polymorphisms. These simple sequence length polymorphisms (SSLP) may be usefully exploited for identity testing, population studies, linkage analysis and genome mapping.
Anatomical studies of the neurovascular bundle and cavernosal nerves (2004)

The current anatomical description of the cavernosal nerve and NVB is inaccurate.
The National Psoriasis Foundation Psoriasis Score (NPF-PS) system versus the Psoriasis Area Severity Index (PASI) and Physician's Global Assessment (PGA): a comparison. (2003)

The Psoriasis Area and Severity Index (PASI) and Physician Global Assessment (PGA) are commonly used, but fail to measure quality of life and the patient's perception of well-being. In response to these limitations, the National Psoriasis Foundation (NPF) Medical Advisory Board has developed the NPF Psoriasis Score (NPF-PS). This article evaluates the degree of concordance between NPF-PS, PASI, and PGA scores via an investigator-initiated, single-center, double-blind, placebo-controlled study of thirty-three patients with moderate to severe plaque psoriasis. Our results indicated that NPF-PS was strongly correlated with PASI and PGA in this study, while better reflecting patient perception. This is the first report of a double-blind placebo-controlled study demonstrating this concordance.
Enzyme therapy in Fabry disease: differential in vivo plasma clearance and metabolic effectiveness of plasma and splenic alpha-galactosidase A isozymes. (1979)

A pilot trial of enzyme replacement with splenic and plasma alpha-galactosidase A (alpha-D-galactosidase; alpha-D-galactoside galactohydrolase, EC 3.2.1.22) isozymes was undertaken in two brothers with Fabry disease, an X-linked glycosphingolipid storage disease. Six unentrapped doses (2000 units/kg) of each isozyme were administered intravenously to the respective recipients during a 117-day period. The circulating half-life of the splenic isozyme was about 10 min, whereas that for the plasma isozyme was approximately 70 min. No immune response was detected by skin and immunodiffusion tests or by alterations in the maximal activity or clearance kinetics for either isozyme after successive administrations. After each dose of the splenic isozyme, the concentration of the accumulated circulating substrate, trihexosylceramide (globotriaosylceramide), decreased maximally (approximately 50% of initial values) in 15 min and returned to preinfusion levels by 2-3 hr. In marked contrast, injection of the plasma isozyme decreased the circulating substrate levels 50-70% by 2-6 hr; the concentrations gradually returned to preinfusion values by 36-72 hr.
<title>Human tooth as an optical device</title> (1991)

The human tooth as an optical system, or more exactly as an optical device, is considered. In this report the authors examine the main properties of such a system.