gebhart/patrepeval huggingface dataset:
Fnac: cómo llevar a cuestas la prevención sin que sea una pesada carga (2007)

A fast silyl derivatization technique for simultaneous GC/ MS analysis of alkylphenols, chlorophenols, and bisphenol A was developed. The analytes were silylized with an excess amount of bis(trimethylsilyl)trifluoroacetamide (BSTFA) followed by hydrolysis of excess silyl reagent with water. Reaction rates of derivatization were studied in various solvents and found to be fastest in acetone. Derivatization reaction in acetone was completed quantitatively within 15 s at room temperature while it took more than 1 h in other solvents studied. Similar results were obtained in mixed solvents with acetone if the content of acetone was higher than 60% (v/v). Since water-immiscible solvents such as dichloromethane or hexane are frequently used in the extraction of phenolic analytes in various sample matrixes, acetone can be added to the extracts in order to accelerate the reaction rate of derivatization. Stability of the derivatives in sample for long-term storage was ensured by hydrolyzing excess derivatizing reagent, BSTFA, with a spike of water followed by dehydration using anhydrous sodium sulfate. On the basis of the above results, a derivatizing treatment kit was designed to improve the convenience of analysis. It was possible to treat sample within several minutes successfully by using the kit. So fast simultaneous determination of those anlaytes by GC/MS was possible with improved convenience as well as sensitivity and reproducibility.
Blood levels of immune cells predict survival in myeloma patients: results of an Eastern Cooperative Oncology Group phase 3 trial for newly diagnosed multiple myeloma patients (2001)

Previously, it was reported that patients with multiple myeloma (MM) who have higher baseline levels of blood CD4(+) or CD19(+) cells have longer survival. This article extends the analysis of immune cell levels and survival in a large cohort (N = 504) of patients with MM entered on Eastern Cooperative Oncology Group (ECOG) phase 3 trial (9486). Newly diagnosed patients with MM received 2 cycles of vincristine, bischloroethylnitrosourea, melphalan, cytoxan, prednisone (VBMCP) and were treated on one of 3 randomized arms: VBMCP with either interferon or high-dose cyclophosphamide, or VBMCP alone. Blood immune cell levels were studied at trial entry (baseline), after 2 cycles of chemotherapy, after 2 years of therapy, and at relapse. Baseline CD3(+), CD4(+), CD8(+), CD19(+), and CD4(+) subset cell levels were all positively associated with survival (P =.0087 to P <.0001). A multivariate analysis incorporating CD4(+) and CD19(+) cell levels defined 3 separate groups of patients with MM to survival outcome. Higher CD19(+) blood levels were positively associated with MM-patient survival at entry to the study, at year 2, and at relapse (P <.0001 at all 3 timepoints). Patients with MM had evidence of immune cell reconstitution after 2 years of therapy, but the rate and extent of recovery was greater for CD8(+), which was greater than CD4(+), which was greater than CD19(+). This latter data affirms the positive relationship between the quantitative status of the blood immune system in MM and survival. In addition, the importance of the CD19(+) blood cells to survival is evident throughout the course of MM. Therapeutic efforts to maintain an intact immune system may be crucial in maximizing chemotherapeutic and/or immunotherapy efforts in this disease.
Preparation and some Properties of HxREBa2CU3O7 (RE = Er, Nd, Dy, Ho, Gd) Superconductors* (1989)

Article Preparation and some Properties of HxREBa2CU3O7 (RE = Er, Nd, Dy, Ho, Gd) Superconductors* was published on February 1, 1989 in the journal Zeitschrift für Physikalische Chemie (volume 163, issue 2).
Ar−F Reductive Elimination from Palladium(II) Revisited (2007)

In contrast with [(o-Tol3P)2Pd2(p-C6H4X)2(μ-F)2] (1; X = NO2; J. Am. Chem. Soc. 2007, 129, 1342), its nonactivated congeners (X = H, Me, and MeO) do not produce 19F NMR-detectable quantities of p-FC6H4X upon thermal decomposition in the presence of Buchwald's ligand t-Bu2P-2-C6H4C6H2(i-Pr)3-2‘,4‘,6‘ (BL). These results do not support the previously asserted “net Ar−F reductive elimination”, given that for X = NO2 some quantities of p-FC6H4X might conceivably be formed from 1 via a variety of other, SNAr-type paths.
A multicast interface for UNIX 4.3 (1988)

Abstract The UNIX 4.3 operating system, like its predecessor UNIX 4.2, is designed to allow interprocess communications between pairs of processes using sockets — pairs of integers identifying specific hosts and ports — which are associated with a process. In this paper a software package which allows interprocess communications between many UNIX processes (as opposed to two) is presented.
An axial velocity estimator for ultrasound blood flow imaging, based on a full evaluation of the Doppler equation by means of a two-dimensional autocorrelation approach (1995)

This paper introduces a new velocity estimator, referred to as the 2D autocorrelator, which differs from conventional Doppler techniques in two respects: the derivation of axial velocity values by evaluating the Doppler equation using explicit estimates of both the mean Doppler and the mean RF frequency at each range gate location; and, the 2D nature (depth samples versus pulse transmissions) of processing within the range gate. The estimator's output can be calculated by evaluating the 2D autocorrelation function of the demodulated (baseband) backscattered echoes at two lags. A full derivation and mathematical description of the estimator is presented, based on the framework of the 2D Fourier transform. The same framework is adopted to analyze two other established velocity estimators (the conventional 1D autocorrelator and the crosscorrelator) in a unifying manner, and theoretical arguments as well as experimental results are used to highlight the common aspects of all three estimators. In addition, a thorough performance evaluation is carried out by means of extensive simulations, which document the effect of a number of factors (velocity spread, range gate length, ensemble length, noise level, transmitted bandwidth) and provide an insight into the optimum parameters and trade-offs associated with individual algorithms. Overall, the 2D autocorrelator is shown to offer the best performance in the context of the specific simulation conditions considered here. Its superiority over the crosscorrelator is restricted to cases of low signal-to-noise ratios. However, the 2D autocorrelator always outperforms the conventional 1D autocorrelator by a significant margin. These comparisons, when linked to the computational requirements of the proposed estimator, suggest that it combines the generally higher performance of 2D broadband time-domain techniques with the relatively modest complexity of 1D narrowband phase-domain velocity estimators.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Monocytes express high amounts of Notch and undergo cytokine specific apoptosis following interaction with the Notch ligand, Delta-1 (2000)

Notch signaling has been shown to play a key role in cell fate decisions in numerous developmental systems. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we reported the expression of human Notch-1 in CD34+ progenitors. In this study, we evaluated the expression of human Notch-1 and Notch-2 protein by hematopoietic cells. In immunofluoresence study, we detected low amounts of Notch-1 and Notch-2 protein in both CD34+ and CD34+Lin- cells, high amounts in CD14+ monocytes as well as B and T cells, but no expression in CD15+ granulocytes. We further found that an immobilized truncated form of the Notch ligand, Delta-1, induced apoptosis in monocytes in the presence of macrophage colony-stimulating factor (M-CSF), but not granulocyte-macrophage colony-stimulating factor (GM-CSF). The widespread expressions of Notch proteins suggest multiple functions for this receptor during hematopoiesis. These studies further indicate a novel role for Notch in regulating monocyte survival. (Blood. 2000;95:2847-2854)
Combinatorial Lead Optimization of [1,2]-Diamines Based on Ethambutol as Potential Antituberculosis Preclinical Candidates (2003)

Despite relatively modest potency, ethambutol (EMB, (S,S)-[N,N-di-2-amino-1-butanol]ethylenediamine) is a mainstay of contemporary chemotherapy for the treatment of tuberculosis. We have developed a solid-phase synthesis of 1,2-diamine analogues of EMB using a novel acylation-reduction sequence that is compatible with high-throughput 96-well format chemistry. Using this procedure, we have synthesized 63 238 diamine analogues in pools of 10 that are suitable for testing. MIC and a target-based reporter assay were used to direct deconvolution of 2796 individual compounds from these mixtures, and the 69 most potent molecules were resynthesized in milligram quantities for hit confirmation. Purification of these individual active diamine analogues allowed the identification of 26 compounds with activity equal to or greater than EMB. Amines which occurred most frequently in active compounds included many with large hydrophobic moieties, suggesting that optimization was perhaps selecting for the isoprenoid binding site of the arabinosyltransferase target of EMB. N-Geranyl-N'-(2-adamantyl)ethane-1,2-diamine (109), the most active of these diamines, displayed a 14-35-fold improvement in activity in vitro against Mycobacterium tuberculosis, as compared to EMB.
The O 2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion (2009)

Fumarate and nitrate reduction regulatory (FNR) proteins are bacterial transcription factors that coordinate the switch between aerobic and anaerobic metabolism. In the absence of O(2), FNR binds a [4Fe-4S](2+) cluster (ligated by Cys-20, 23, 29, 122) promoting the formation of a transcriptionally active dimer. In the presence of O(2), FNR is converted into a monomeric, non-DNA-binding form containing a [2Fe-2S](2+) cluster. The reaction of the [4Fe-4S](2+) cluster with O(2) has been shown to proceed via a 2-step process, an O(2)-dependent 1-electron oxidation to yield a [3Fe-4S](+) intermediate with release of 1 Fe(2+) ion, followed by spontaneous rearrangement to the [2Fe-2S](2+) form with release of 1 Fe(3+) and 2 S(2-) ions. Here, we show that replacement of Ser-24 by Arg, His, Phe, Trp, or Tyr enhances aerobic activity of FNR in vivo. The FNR-S24F protein incorporates a [4Fe-4S](2+) cluster with spectroscopic properties similar to those of FNR. However, the substitution enhances the stability of the [4Fe-4S](2+) cluster in the presence of O(2). Kinetic analysis shows that both steps 1 and 2 are slower for FNR-S24F than for FNR. A molecular model suggests that step 1 of the FNR-S24F iron-sulfur cluster reaction with O(2) is inhibited by shielding of the iron ligand Cys-23, suggesting that Cys-23 or the cluster iron bound to it is a primary site of O(2) interaction. These data lead to a simple model of the FNR switch with physiological implications for the ability of FNR proteins to operate over different ranges of in vivo O(2) concentrations.
Mesenchymal stem cells (MSC) as therapeutic cytoreagents for gene therapy (2005)

We developed human mesenchymal stem cell (MSC) lines that could differentiate into various tissue cells including bone, neural cells, bone marrow (BM) stromal cells supporting the growth of hematopoietic stem cell (HSC), and so-called 'tumor stromal cells' mixing with tumor cells. We investigated the applicability of MSC as therapeutic cell transplanting reagents (cytoreagents). Telomerized human BM derived stromal cells exhibited a prolonged lifespan and supported the growth of hematopoietic clonogenic cells. The gene transfer of Indian hedgehog (Ihh) remarkably enhanced the HSC expansion supported by the human BM stromal cells. Gene-modified MSC are useful as therapeutic tools for brain tissue damage (e.g. brain infarction) and malignant brain neoplasms. MSC transplantation protected the brain tissue from acute ischemic damage in the midcerebral artery occlusion (MCAO) animal model. Brain-derived neurotrophic factor (BDNF)-gene transduction further enhanced the protective efficacy against the ischemic damage. MSC possessed excellent migratory ability and exerted inhibitory effects on the proliferation of glioma cells. Gene-modification of MSC with therapeutic cytokines clearly augmented the antitumor effect and prolonged the survival of tumor-bearing animals. Gene therapy employing MSC as a tissue-protecting and targeting cytoreagent would be a promising approach.
Immunosensors: technology and opportunities in laboratory medicine (1996)

An immunosensor is a device comprising an antigen or antibody species coupled to a signal transducer, which detects the binding of the complementary species. An indirect immunosensor uses a separate labeled species that is detected after binding by, e.g., fluorescence or luminescence (i.e., a heterogeneous immunoassay). A direct device detects the binding by a change in potential difference, current, resistance, mass, heat, or optical properties (i.e., a homogeneous immunoassay). Although indirect sensors may encounter fewer problems due to nonspecific binding effects, the direct sensors are capable of real-time monitoring of the antigen-antibody reaction. A wide range of molecules can be detected with detection limits ranging between 10(-9) and 10(-13) mol/L. However, there are only a few successful commercial applications of direct immunosensors, these being of the optical type. This review describes the principles underlying the technologies, their merits, limitations, and applications.
Living Ring-Opening Metathesis Polymerization in Water (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTLiving Ring-Opening Metathesis Polymerization in WaterDavid M. Lynn, Bernhard Mohr, and Robert H. GrubbsView Author Information Arnold and Mabel Beckman Laboratories of Chemical Synthesis Division of Chemistry and Chemical Engineering California Institute of Technology Pasadena, California 91125 Cite this: J. Am. Chem. Soc. 1998, 120, 7, 1627–1628Publication Date (Web):February 4, 1998Publication History Received20 October 1997Published online4 February 1998Published inissue 1 February 1998https://pubs.acs.org/doi/10.1021/ja9736323https://doi.org/10.1021/ja9736323rapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views2475Altmetric-Citations194LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Catalysts,Group 15 compounds,Living polymerization,Monomers,Organic reactions Get e-Alerts
The recombinant catalytic domain of membrane‐type matrix metalloproteinase‐1 (MT1‐MMP) induces activation of progelatinase A and progelatinase A complexed with TIMP‐2 (1996)

A truncated form of the membrane-type matrix metalloproteinase-1 [(Ala21-Ile318)proMT1-MMP] lacking the hemopexin-like and trans-membrane domain was produced in E. coli. We demonstrate that the recombinant proenzyme was autoproteolytically processed to a fully active catalytic domain with N-terminal Ile114. The catalytic domain of MT1-MMP initiated the activation of progelatinase A and progelatinase A complexed with tissue inhibitor of metalloproteinases-2 (TIMP-2). As a typical soluble metalloproteinase it was able to cleave physiologic as well as synthetic substrates. Our kinetic data demonstrate that TIMP-2 is a potent inhibitor for the recombinant enzyme.
Human Retinal Disease fromAIPL1Gene Mutations: Foveal Cone Loss with Minimal Macular Photoreceptors and Rod Function Remaining (2010)

AIPL1-LCA, unlike some other forms of LCA with equally severe visual disturbance, shows profound loss of foveal as well as extrafoveal photoreceptors. The more unusual late-onset and slower form of AIPL1 disease may be better suited to gene augmentation therapy and is worthy of detection and further study.
Subsolidus Equilibria in the System ZrO 2 ‐WO 3 ‐P 2 O 5 (1970)

The existence of stable and metastable forms of 2ZrO 2 ·P 2 O 5 and the subsolidus phase relations in the system ZrO 2 ‐ZrP 2 O 7 were confirmed before investigation of the ternary system. The synthesis and thermal behavior of ZrW 2 O 8 were reinvestigated, and the system WO 3 ‐P 2 O 5 was examined cursorily. A ternary compound, 2ZrO 2 ·WO 3 ·P 2 O 5 , was found, and compatibility triangles for the system between 1105° and 1150°C were established. The ternary compound is compatible with ZrO 2 , WO 3 , and three binary compounds, giving rise to five composition triangles. In addition, ZrP 2 O 7 , WO 3 , and “W 2 O 3 (PO 4 ) 2 ” were compatible.
Development and activation of regulatory T cells in the human fetus (2005)

There is an increasing amount of knowledge on the functional properties of regulatory T cells (Treg) in the adult immune system, but data on the generation and function of these cells during human embryonic development are scarce. In this study, we show that in the fetal thymus, double-positive cells initiate expression of CD25, GITR, CTLA4 and CD122 during their transition from the CD27- to the CD27+ stage. Moreover, CD4+CD25+ fetal thymocytes already have the potential to suppress proliferation of CD25- cells. After leaving the thymus, FoxP3+CD4+CD25+ Treg enter the fetal lymph nodes and spleen, where they acquire a primed/memory phenotype. A model is proposed for the development of human fetal Treg that encompasses two sequential maturation steps: initiation of a regulatory phenotype and suppressive activity in the thymus; and subsequent activation within the peripheral lymphoid organs. Upon activation, FoxP3+CD4+CD25+ Treg suppress potentially deleterious responses by autoreactive lymphocytes and maintain homeostasis within the developing fetus.
Injection of Sperm Cytosolic Factor Into Mouse Metaphase II Oocytes Induces Different Developmental Fates According to the Frequency of [Ca2+]i Oscillations and Oocyte Age1 (2000)

Intracellular calcium ([Ca(2+)](i)) rises are a hallmark of mammalian fertilization and are associated with normal activation of embryonic development. Injection of mammalian sperm cytosolic factor (SCF) into oocytes has been shown to trigger [Ca(2+)](i) rises similar to those observed during fertilization, and to initiate normal embryonic development. However, Ca(2+) release has also been shown to be associated with cell death, but the mechanisms of the detrimental effects of Ca(2+) stimulation on development have not yet been investigated. Thus, studies were undertaken using SCF to test the effects of [Ca(2+)](i) oscillations on oocyte activation in freshly ovulated and aged oocytes. Injections of 1 mg/ml SCF into freshly ovulated mouse metaphase II oocytes, which evoked Ca(2+) responses with low frequency and short duration, induced normal activation and cleavage to the two-cell stage. Conversely, injection of 15 mg/ml SCF, which triggered high-frequency and persistent Ca(2+) responses, induced abnormal activation that was characterized by abnormal chromatin configurations, inhibition of DNA synthesis, and lack of first mitotic spindle assembly. More importantly, fertilization-like Ca(2+) responses induced by injection of 1 mg/ml SCF triggered cell death, rather than activation, in in vitro-aged oocytes. These oocytes exhibited extensive cytoplasmic and DNA fragmentation that was accompanied by activation of protein caspases, all of which are signs of apoptotic cell death. Fewer similarly aged oocytes that were either unstimulated or activated with 7% ethanol underwent fragmentation. Together, these results suggest that [Ca(2+)](i) oscillations are required to activate freshly ovulated oocytes, but if initiated at abnormally high frequency and duration or if induced in aged oocytes, the [Ca(2+)](i) oscillations may trigger premature termination of embryonic development.
Spatial multiplexing in correlated fading via the virtual channel representation (2003)

Spatial multiplexing techniques send independent data streams on different transmit antennas to maximally exploit the capacity of multiple-input multiple-output (MIMO) fading channels. Most existing multiplexing techniques are based on an idealized MIMO channel model representing a rich scattering environment. Realistic channels corresponding to scattering clusters exhibit correlated fading and can significantly compromise the performance of such techniques. In this paper, we study the design and performance of spatial multiplexing techniques based on a virtual representation of realistic MIMO fading channels. Since the nonvanishing elements of the virtual channel matrix are uncorrelated, they capture the essential degrees of freedom in the channel and provide a simple characterization of channel statistics. In particular, the pairwise-error probability (PEP) analysis for correlated channels is greatly simplified in the virtual representation. Using the PEP analysis, various precoding schemes are introduced to improve performance in virtual channels. Unitary precoding is proposed to provide robustness to unknown channel statistics. Nonunitary precoding techniques are proposed to exploit channel structure when channel statistics are known at the transmitter. Numerical results are presented to illustrate the attractive performance of the precoding techniques.