gebhart/patrepeval huggingface dataset:
Engine Oil Viscosity Sensors Using Disks of PZT Ceramic as Electromechanical Vibrators (1997)

&lt;div class="htmlview paragraph"&gt;Experimental forms of two different types of engine oil viscosity sensors have been tested that use uniformly poled disks of piezoelectric PZT ceramic. In both cases, the disks were used to form electromechanical resonators functioning as the frequency-controlling element in a transistor oscillator circuit. The simpler type of sensor used only one disk, vibrating in a radial-longitudinal mode of vibration. In this mode, a disk 2.54 cm in diameter and 0.127 cm thick had a resonant frequency of approximately 90 kHz. The second type of sensor used two such disks bonded together by a conducting epoxy, with poling directions oriented in opposite directions. This composite resonator vibrated in a radially-symmetrical, flexural mode of vibration, with the lowest resonant frequency at approximately 20 kHz. The presence of tangential components of motion on the major faces of both resonators made them sensitive to the viscosity of fluids in which they were immersed. Laboratory measurements indicated that both types of resonators, when immersed in engine oil samples, were capable of being used as viscosity sensors. Viscosity data for new and used engine oils are discussed.&lt;/div&gt;
Characterization of human bone marrow‐derived closed circular DNA clones (1993)

Because of interest in mechanisms of recombination involved in chromosomal deletions in neoplastic disease, and their relation to possible rearrangements in normal tissues, we are studying circular DNA molecules from human tissue with a long-term goal of investigating them as possible by-products of physiologically relevant intrachromosomal recombination events. Covalently closed circular (ccc) DNA from human bone marrow was cloned in bacteriophage vectors, and fourteen clones chosen randomly from the cccDNA-derived library were characterized. Five clones originated from chromosome-specific centromeric alpha-satellite DNA; two clones carried highly repetitive sequences probably derived from interspersed repetitive elements; six clones were derived from single-copy chromosome-specific sequences which detected homologous rodent sequences; and one clone (EPM10) was derived from a small chromosome 11-specific sequence family which localized to chromosome regions 11cen and 11q14. Oligonucleotide primers derived from the cccDNA clones were used in polymerase chain reaction studies to show that (1) the EPM10 clone carried the circular junction, (2) several of the single-copy products could be detected in three different bone marrow cccDNA preparations, and (3) the Alu-PCR profile for bone marrow cccDNA showed distinct bands which were similar in four bone marrow cccDNA preparations.
bcl-2 Transgene Expression Inhibits Apoptosis in the Germinal Center and Reveals Differences in the Selection of Memory B Cells and Bone Marrow Antibody-Forming Cells (2000)

Immunization with T cell-dependent antigens generates long-lived memory B cells and antibody-forming cells (AFCs). Both populations originate in germinal centers and, predominantly, produce antibodies with high affinity for antigen. The means by which germinal center B cells are recruited into these populations remains unclear. We have examined affinity maturation of antigen-specific B cells in mice expressing the cell death inhibitor bcl-2 as a transgene. Such mice had reduced apoptosis in germinal centers and an excessive number of memory B cells with a low frequency of V gene somatic mutation, including those mutations encoding amino acid exchanges known to enhance affinity. Despite the frequency of AFCs being increased in bcl-2-transgenic mice, the fraction secreting high-affinity antibody in the bone marrow at day 42 remained unchanged compared with controls. The inability of BCL-2 to alter selection of bone marrow AFCs is consistent with these cells being selected within the germinal center on the basis of their affinity being above some threshold rather than their survival being due to a selective competition for an antigen-based signal. Continuous competition for antigen does, however, explain formation of the memory compartment.
Overexpression of Matrix Metalloproteinase 11 in Thai Prostatic Adenocarcinoma is Associated with Poor Survival (2013)

Expression of MMP-11 is significantly associated with survival in prostatic adenocarcinoma. High levels may potentially be used for prediction of a poor prognosis.
Translocation of Pro-OmpA across Inner Membrane Vesicles of Escherichia coli Occurs in Two Consecutive Energetically Distinct Steps (1989)

The rate of energy-dependent transfer of pro-OmpA across Escherichia coli inner membrane vesicles in vitro was found to be a function of the ATP concentration. At concentrations above 0.1 mM ATP, the addition of a transmembrane electrochemical potential (proton motive force or pmf) increased the rate of pro-OmpA translocation. Additional experiments demonstrated that the overall reaction proceeded by at least two distinct energy-requiring steps. The first step required only ATP, was nearly unaffected by the pmf, and resulted in the insertion of the amino-terminal domain of pro-OmpA across the membrane. The insertion exposed the signal sequence cleavage site to the periplasmic side of the membrane, as measured by the appearance of a mature length translocation intermediate. However, this intermediate was partially exposed to the cytoplasmic side of the membrane. In a second energy-dependent step, either ATP or the pmf was sufficient to complete the translocation of mature length OmpA across the membrane.
Rapid Cycling of Lipid Raft Markers between the Cell Surface and Golgi Complex (2001)

The endocytic itineraries of lipid raft markers, such as glycosyl phosphatidylinositol (GPI)-anchored proteins and glycosphingolipids, are incompletely understood. Here we show that different GPI-anchored proteins have different intracellular distributions; some (such as the folate receptor) accumulate in transferrin-containing compartments, others (such as CD59 and GPI-linked green fluorescent protein [GFP]) accumulate in the Golgi apparatus. Selective photobleaching shows that the Golgi pool of both GPI-GFP and CD59-GFP constantly and rapidly exchanges with the pool of these proteins found on the plasma membrane (PM). We visualized intermediates carrying GPI-GFP from the Golgi apparatus to the PM and separate structures delivering GPI-GFP to the Golgi apparatus.GPI-GFP does not accumulate within endocytic compartments containing transferrin, although it is detected in intracellular structures which are endosomes by the criteria of accessibility to a fluid phase marker and to cholera and shiga toxin B subunits (CTxB and STxB, which are also found in rafts). GPI-GFP and a proportion of the total CTxB and STxB taken up into cells are endocytosed independently of clathrin-associated machinery and are delivered to the Golgi complex via indistinguishable mechanisms. Hence, they enter the Golgi complex in the same intermediates, get there independently of both clathrin and rab5 function, and are excluded from it at 20 degrees C and under conditions of cholesterol sequestration. The PM-Golgi cycling pathway followed by GPI-GFP could serve to regulate lipid raft distribution and function within cells.
Receptor mediated uptake of peptides that bind the human transferrin receptor (2001)

A biopanning process designed to find peptide epitopes specific for cell surface receptors has been used in this study to select seven- and 12-amino-acid peptides capable of binding to and internalizing with the human transferrin receptor (hTfR). Through sequential rounds of negative and positive selection, two peptide sequences were identified that specifically bind to the hTfR. Phage containing the sequences HAIYPRH or THRPPMWSPVWP were inhibited from binding the hTfR in a dose-dependent fashion when peptides of the same sequence were present in a competition assay. Interestingly, transferrin did not compete with either of these sequences for receptor binding, suggesting that these peptides bind a site on the hTfR distinct from the transferrin binding site. When either of these sequences was expressed as a fusion to green fluorescent protein (GFP), the recombinant GFP molecule was internalized in cells expressing the hTfR. These studies suggest that the two peptides can be used to target other proteins into the endosomal pathway. Further, they provide a strategy for identifying peptides that bind to other cell surface receptors that can be used for both diagnostic and therapeutic purposes.
Phytochrome, a family of photoreceptors with multiple physiological roles (1990)

Abstract. Photoperception by phytochrome is crucially important at many stages of the plant life cycle in allowing adaptation to a changing light environment. Phytochrome is encoded by a family of genes subject to differential expression in response to environmental and developmental factors. Multiple forms of phytochrome exist with, in some cases, identifiably different spectrophotometric, biochemical and physiological characteristics. This article reviews the regulation of development by phytochrome and discusses evidence from physiological studies of wild type, mutant and transgenic plants consistent with the proposal that different members of the phytochrome family have different photosensory roles. We speculate briefly on the possible evolutionary relationship between the different photosensory roles, and suggest approaches towards further elucidation of the nature of phytochrome‐mediated photoperception and adaptation to the natural light environment.
Making Mix Nets Robust For Electronic Voting By Randomized Partial Checking. (2002)

We propose a new technique for making mix nets robust, called randomized partial checking (RPC). The basic idea is that rather than providing a proof of completely correct operation, each server provides strong evidence of its correct operation by revealing a pseudorandomly selected subset of its input/output relations. Randomized partial checking is exceptionally e#cient compared to previous proposals for providing robustness; the evidence provided at each layer is shorter than the output of that layer, and producing the evidence is easier than doing the mixing. It works with mix nets based on any encryption scheme (i.e., on public-key alone, and on hybrid schemes using public-key/symmetric-key combinations). It also works both with Chaumian mix nets where the messages are successively encrypted with each servers&amp;apos; key, and with mix nets based on a single public key with randomized re-encryption at each layer.
Simultaneous Deep Transfer Across Domains and Tasks (2015)

Recent reports suggest that a generic supervised deep CNN model trained on a large-scale dataset reduces, but does not remove, dataset bias. Fine-tuning deep models in a new domain can require a significant amount of labeled data, which for many applications is simply not available. We propose a new CNN architecture to exploit unlabeled and sparsely labeled target domain data. Our approach simultaneously optimizes for domain invariance to facilitate domain transfer and uses a soft label distribution matching loss to transfer information between tasks. Our proposed adaptation method offers empirical performance which exceeds previously published results on two standard benchmark visual domain adaptation tasks, evaluated across supervised and semi-supervised adaptation settings.
Catalytic Production of High-Grade Fuel (Gasoline) from Biomass Compounds by Shape-Selective Catalysis (1979)

Shape-selective hydrocarbon catalysis, such as the conversion capability of zealite catalysts of the ZSM-5 type to produce high-grade gasoline from methanol, can be extended to produce a similar gasoline or aromatics from plant extracts. Examples are rubber latex, corn oil, and peanut oil. Novel mechanisms for shape-selective reaction sequences are demonstrated.
Development of a rapid and specific colony-lift immunoassay for detection and enumeration of Campylobacter jejuni, C. coli, and C. lari (1996)

Contamination of retail poultry by Campylobacter spp. is a significant source of human diarrheal disease. We have developed a colony-lift immunoassay (CLI) for the detection of Campylobacter jejuni, C. coli, and C. lari isolated from such sources and grown on selective agar medium or on filter membranes. This technique has been successfully utilized to quantify Campylobacter colonies within 18 to 28 h after sampling. Hydrophobic, high-protein-binding membranes were prewet with methanol and used to imprint bacterial cells from the agar or filter membrane, while leaving colonies intact and viable. The membranes were air dried, peroxidase neutralized, blocked with bovine serum albumin in phosphate-buffered saline, and hybridized for 5 min with an affinity-purified, horseradish peroxidase-labeled goat anti-Campylobacter antibody preparation (Kirkegaard and Perry Laboratories). The membranes were washed briefly, exposed to a 3,'5,5'-tetramethylbenzidine membrane substrate, rinsed in deionized water, and allowed to dry. Lifted colonies of Campylobacter were identified by a blue color reaction on the membrane. Replicas of the membranes were made by marking the location of the Campylobacter colonies on clear transparencies, which were subsequently utilized to locate the original colony on the filter membrane or agar plate. The specificity of this antibody preparation has been evaluated against a wide range of Campylobacter spp., including American Type Culture Collection type and references strains, retail poultry isolates, and isolates obtained from cloacal swabs of live commercial broiler chickens. Specificity against numerous non-Campylobacter spp. obtained from the same sources was also evaluated. The CLI provided a rapid and simple means for detection and enumeration of enteropathogenic Campylobacter organisms. We have successfully combined this CLI procedure with methods recently developed in our laboratories for retail meat and poultry sampling. Potentially, broader applications for use of this technique include detection and enumeration of campylobacters from clinical, veterinary, and environmental samples.
Recent Application of Probiotics in Food and Agricultural Science (2012)

Lactobacillus and Bifidobacterium are most commonly used probiotics in food and feed (Table Other microorganisms such as yeast Saccharomyces cerevisiae and some Escherichia coli and Bacillus species are also used as probiotics. Lactic acid bacteria (LAB) which have been used for food fermentation since the ancient time, can serve a dual function by acting as food fermenting agent and potentially health benefits provider. LAB are GRAS (general recognized as safe) with no pathogenic, or virulence properties have been reported. For the use of LAB as probiotics, some desirable characteristics such as low cost, maintaining its viability during the processing and storage, facility of the application in the products, resistance to the physicochemical processing must be considered.
Interaction of C1q and Mannan-Binding Lectin (MBL) with C1r, C1s, MBL-Associated Serine Proteases 1 and 2, and the MBL-Associated Protein MAp19 (2000)

Mannan-binding lectin (MBL) and C1q activate the complement cascade via attached serine proteases. The proteases C1r and C1s were initially discovered in a complex with C1q, whereas the MBL-associated serine proteases 1 and 2 (MASP-1 and -2) were discovered in a complex with MBL. There is controversy as to whether MBL can utilize C1r and C1s or, inversely, whether C1q can utilize MASP-1 and 2. Serum deficient in C1r produced no complement activation in IgG-coated microwells, whereas activation was seen in mannan-coated microwells. In serum, C1r and C1s were found to be associated only with C1q, whereas MASP-1, MASP-2, and a third protein, MAp19 (19-kDa MBL-associated protein), were found to be associated only with MBL. The bulk of MASP-1 and MAp19 was found in association with each other and was not bound to MBL or MASP-2. The interactions of MASP-1, MASP-2, and MAp19 with MBL differ from those of C1r and C1s with C1q in that both high salt concentrations and calcium chelation (EDTA) are required to fully dissociate the MASPs or MAp19 from MBL. In the presence of calcium, most of the MASP-1, MASP-2, and MAp19 emerged on gel-permeation chromatography as large complexes that were not associated with MBL, whereas in the presence of EDTA most of these components formed smaller complexes. Over 95% of the total MASPs and MAp19 found in serum are not complexed with MBL.
G-protein-coupled receptors for neurotransmitter amino acids: C-terminal tails, crowded signalosomes (2002)

G-protein-coupled receptors (GPCRs) represent a superfamily of highly diverse integral membrane proteins that transduce external signals to different subcellular compartments, including nuclei, via trimeric G-proteins. By differential activation of diffusible G(alpha) and membrane-bound G(beta)gamma subunits, GPCRs might act on both cytoplasmic/intracellular and plasma-membrane-bound effector systems. The coupling efficiency and the plasma membrane localization of GPCRs are regulated by a variety of interacting proteins. In this review, we discuss recently disclosed protein interactions found with the cytoplasmic C-terminal tail regions of two types of presynaptic neurotransmitter receptors, the group III metabotropic glutamate receptors and the gamma-aminobutyric acid type-B receptors (GABA(B)Rs). Calmodulin binding to mGluR7 and other group III mGluRs may provide a Ca(2+)-dependent switch for unidirectional (G(alpha)) versus bidirectional (G(alpha) and G(beta)gamma) signalling to downstream effector proteins. In addition, clustering of mGluR7 by PICK1 (protein interacting with C-kinase 1), a polyspecific PDZ (PSD-95/Dlg1/ZO-1) domain containing synaptic organizer protein, sheds light on how higher-order receptor complexes with regulatory enzymes (or 'signalosomes') could be formed. The interaction of GABA(B)Rs with the adaptor protein 14-3-3 and the transcription factor ATF4 (activating transcription factor 4) suggests novel regulatory pathways for G-protein signalling, cytoskeletal reorganization and nuclear gene expression: processes that may all contribute to synaptic plasticity.
Relationships Between the Chemical Structure of Antimycobacterial Substances and Their Activity Against Atypical Strains. Part 14: 3-Aryl-6,8-dihalogeno-2H-1,3-benzoxazine-2,4(3H)-diones (1998)

A set of eight derivatives of 6,8-dichloro-3-phenyl-2H-benzoxazine-2,4(3H)-dione and nine derivatives of 6,8-dibromo-3-phenyl-2H-1, 3-benzoxazine-2,4(3H)-dione, substituted on the phenyl ring, was prepared by the reaction of the corresponding salicylanilides with ethyl chloroformate. The compounds were evaluated in vitro for antimycobacterial activity against Mycobacterium tuberculosis, Mycobacterium kansasii, and Mycobacterium avium. Their activity increases with increasing hydrophobicity and electron-withdrawing ability of the substituents on the phenyl ring.
Reduced resolution residual coding for H.264-based compression system (2006)

The current state-of-the-art video coding standard, H.264, offers substantially better compression performance than earlier video coding standards. However, even with these gains, better compression is still desirable. This paper describes a macroblock-based mixed resolution video encoding system. By reducing the spatial resolution of some macroblock residuals intelligently, our preliminary experiments show up to 20% improvement in video compression efficiency compared to H.264 while maintaining good subjective video quality.
Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy (2010)

The autosomal recessive kidney disease nephronophthisis (NPHP) constitutes the most frequent genetic cause of terminal renal failure in the first 3 decades of life. Ten causative genes (NPHP1-NPHP9 and NPHP11), whose products localize to the primary cilia-centrosome complex, support the unifying concept that cystic kidney diseases are "ciliopathies". Using genome-wide homozygosity mapping, we report here what we believe to be a new locus (NPHP-like 1 [NPHPL1]) for an NPHP-like nephropathy. In 2 families with an NPHP-like phenotype, we detected homozygous frameshift and splice-site mutations, respectively, in the X-prolyl aminopeptidase 3 (XPNPEP3) gene. In contrast to all known NPHP proteins, XPNPEP3 localizes to mitochondria of renal cells. However, in vivo analyses also revealed a likely cilia-related function; suppression of zebrafish xpnpep3 phenocopied the developmental phenotypes of ciliopathy morphants, and this effect was rescued by human XPNPEP3 that was devoid of a mitochondrial localization signal. Consistent with a role for XPNPEP3 in ciliary function, several ciliary cystogenic proteins were found to be XPNPEP3 substrates, for which resistance to N-terminal proline cleavage resulted in attenuated protein function in vivo in zebrafish. Our data highlight an emerging link between mitochondria and ciliary dysfunction, and suggest that further understanding the enzymatic activity and substrates of XPNPEP3 will illuminate novel cystogenic pathways.
Error detection in fault secure controllers using state encoding (1996)

In applications where safety is of utmost importance, defective functions must frequently be detected very early in order to restore the system to a safe operating condition. At the same time the availability may not be restricted by measures implemented to detect errors. The efficiency of error detection state encodings for a control unit is investigated based on the example of the Motorola microprocessor core M68HC11. The terms dependent encoding and independent encoding are introduced. The designed circuits are compared with regard to error detection of stuck-at errors and chip area. The degree of error detection is shown with the help of a fault simulation.
Pharmacological HIF‐PHD inhibition reduces renovascular resistance and increases glomerular filtration by stimulating nitric oxide generation (2021)

Our data provide mechanistic insights into dose-dependent effects of short-term pharmacological HIF activation on renal haemodynamics, glomerular filtration and O<sub>2</sub> metabolism and identify NO as a major mediator of these effects.
Palladium Catalyzed Regioselective β-Acetonation−α-Allylation of Activated Olefins in One Shot (1998)

The reaction of certain activated olefins 4 with allyl acetoacetate 5 in the presence of catalytic amounts of Pd(PPh3)4 (5 mol %) in THF at room temperature gave the corresponding β-acetonated α-allylated double addition products 6 regioselectively in good to excellent yields. The nature of the electron-withdrawing group in activated olefins affected significantly the reactivity of substrates; at least one of two electron-withdrawing groups of 4 should be a CN group. A proposed mechanism for this unprecedented three-component coupling reaction involves oxa-π-allyl-π-allylpalladium intermediate 3a (or its synthetic equivalents 3b−d). The in situ generation of activated olefins 4, from the aldehyde 11 and malononitrile 12, followed by the palladium-catalyzed reaction with allyl acetoacetate 5 also worked well, producing the corresponding three-component coupling products in good yields. Furthermore, allyltributylstannane 13 and α-chloro acetone 14 could be used as the α-allylation and β-acetonation components, respectively, instead of allyl acetoacetate 5. The scope and limitations of palladium-catalyzed regioselective β-acetonation−α-allylation reaction of activated olefins are described.