gebhart/patrepeval huggingface dataset:
Tissue Engineering of Small IntestineCurrent Status (2006)

Short bowel syndrome (SBS) has always posed a great threat to patients and has been one of the biggest challenges for doctors due to its high morbidity and mortality. So far, parenteral nutrition (PN) and small bowel transplantation remain the only viable therapeutic options. However, sepsis and liver failure associated with PN and limited availability of the donor organs and high graft rejection rates associated with transplantation have limited their use to a nonpermanent solution. Clearly, there is a need for an alternative therapy whereby increasing the absorptive surface area would help neonates and adults suffering from permanent intestinal failure. Techniques such as sequential intestinal lengthening are being explored in animal models with little success. Attempts to engineer small intestine since the late 1980s have achieved varying degrees of success in animal models with evolving refinements in biotechnology. The most encouraging results so far have been the generation of intestinal neomucosa in the form of cysts when intestinal epithelial organoid units isolated from neonatal rats were seeded onto biodegradable polymers before implantation in syngeneic adult rats' omentum. Although still experimental, continued attempts worldwide using cultured stem cells and improved polymer technology offer promise to provide an off-the-shelf artificial intestine as a novel therapy for patients with SBS. This article reviews the current status of progress in the field of small intestinal tissue engineering and addresses various types of cell sources and scaffold material having potential to be used in this field.
Abstract: Seismic Direct Hydrocarbon Indicators in the Malaysia-Thailand Joint Development Area (2001)

Abstract The use of seismic amplitudes in finding hydrocarbons in Tertiary clastic basins is well established. In this respect explorers in the Gulf of Thailand have experienced an exceptional track record of success. This paper provides a summary of how seismic Direct Hydrocarbon Indicators (DHI's) are being used in the Malaysia- Thailand Joint Development Area (MTJDA) to reduce risk through the entire cycle of Exploration through Appraisal and into Development. Since 1995 sixteen wells have been drilled in the block without a single failure to flow hydrocarbons to the surface. In all cases the primary tool for identifying drillable targets has been the presence of amplitude anomalies that have conformed to structure. In the forward exploration program, amplitude anomalies continue to be a primary criterion for defining drillable prospects, but with the notable difference that trapping involves stratigraphic components. In the appraisal of the gas holding areas which have been established around the exploration wells, quantitative and qualitative analysis of amplitude anomalies, with the aid of 3D seismic, forms the basis for determining the P1, P2 and P3 reserves and for deciding the optimum locations for the appraisal drilling program. For the development program 3D seismic data has been used extensively to provide the framework for the reservoir model as well as measurements of reservoir quality that have been tied to seismic amplitude variations. In addition, seismic amplitude and estimates of acoustic impedance derived from seismic are being used to select all development drilling targets. Despite the success in using seismic amplitudes, a number of significant challenges remain. In particular, success is hampered by the ubiquitous presence of shallow gas, by the limitations imposed by the resolution of the seismic and by the presence of numerous thin coal beds that produce false-positive DHI responses. Together with an overview of the DHI successes, this paper provides examples of the principal limiting factors and some of the approaches being taken to address them. The relative merits of 2D versus 3D seismic and of various data attribute volumes and analysis techniques are discussed. Particular emphasis is placed on applying an integrated methodology that requires careful ties between seismic and wells and fully incorporates the geological model. Presented at: 2003 South East Asia Petroleum Exploration Society (SEAPEX) Conference, Singapore, 2003
History of electric vehicles in General Motors (1994)

This paper traces the efforts by General Motors in the area of electric and hybrid propulsion systems. Propulsion systems developed for different vehicles are described and performance achieved is presented. The activities to commercialize electric vehicles are briefly examined. The paper concludes with recommendations of technologies to be applied in propulsion systems to bring the performance of the electric vehicles to the state that is viable for mass acceptance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Membrane-Type Acoustic Metamaterial with Negative Dynamic Mass (2008)

We present the experimental realization and theoretical understanding of a membrane-type acoustic metamaterial with very simple construct, capable of breaking the mass density law of sound attenuation in the 100-1000 Hz regime by a significant margin ( approximately 200 times). Owing to the membrane's weak elastic moduli, there can be low-frequency oscillation patterns even in a small elastic film with fixed boundaries defined by a rigid grid. The vibrational eigenfrequencies can be tuned by placing a small mass at the center of the membrane sample. Near-total reflection is achieved at a frequency between two eigenmodes where the in-plane average of normal displacement is zero. By using finite element simulations, negative dynamic mass is explicitly demonstrated at frequencies around the total reflection frequency. Excellent agreement between theory and experiment is obtained.
Immune-Based Therapies for Treatment of HIV Infection (1996)

Immune-based agents represent a new approach to the treatment of HIV infection. Whereas antiretrovirals only inhibit viral replication, these agents are designed to enhance the immune system of the patient. Future attempts to manage HIV infection may combine standard nucleoside analogs with immune-based therapies.
Integrin α6β4 identifies an adult distal lung epithelial population with regenerative potential in mice (2011)

Laminins and their integrin receptors are implicated in epithelial cell differentiation and progenitor cell maintenance. We report here that a previously unrecognized subpopulation of mouse alveolar epithelial cells (AECs) expressing the laminin receptor α6β4, but little or no pro-surfactant C (pro-SPC), is endowed with regenerative potential. Ex vivo, this subpopulation expanded clonally as progenitors but also differentiated toward mature cell types. Integrin β4 itself was not required for AEC proliferation or differentiation. An in vivo embryonic lung organoid assay, which we believe to be novel, was used to show that purified β4+ adult AECs admixed with E14.5 lung single-cell suspensions and implanted under kidney capsules self-organized into distinct Clara cell 10-kDa secretory protein (CC10+) airway-like and SPC+ saccular structures within 6 days. Using a bleomycin model of lung injury and an SPC-driven inducible cre to fate-map AECs, we found the majority of type II AECs in fibrotic areas were not derived from preexisting type II AECs, demonstrating that SPC- progenitor cells replenished type II AECs during repair. Our findings support the idea that there is a stable AEC progenitor population in the adult lung, provide in vivo evidence of AEC progenitor cell differentiation after parenchymal injury, and identify a strong candidate progenitor cell for maintenance of type II AECs during lung repair.
Roles of Prostaglandin I 2 and Thromboxane A 2 in Cardiac Ischemia-Reperfusion Injury (2001)

PGI(2), which was produced endogenously during cardiac I/R, exerts a protective effect on cardiomyocytes independent of its effects on platelets and neutrophils. In contrast, TXA(2) has little role in the cardiac I/R injury.
Soluble PD-1 Facilitates 4-1BBL–Triggered Antitumor Immunity against Murine H22 Hepatocarcinoma In vivo (2007)

Our findings in this report imply a great potential of 4-1BBL in combination with sPD-1 in tumor therapeutics with the in vivo existent tumor cells as antigens.
Mantle cell lymphoma. (1994)

Article Tools Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.1994.12.4.876 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 8151331 Mantle cell lymphoma. N L HarrisxN L HarrisSearch for articles by this author https://doi.org/10.1200/JCO.1994.12.4.876 PDF "Mantle cell lymphoma.." Journal of Clinical Oncology, 12(4), pp. 876–877© 1994 by American Society of Clinical Oncology
Changes in vasopressin use and outcomes in surgical intensive care unit patients with septic shock. (2010)

Vasopressin is recently used at lower doses and in less severe septic shock. Patients recently treated with vasopressin have a higher SICU survival rate than the survival rate when vasopressin was first introduced for septic shock.
Frequent loss of imprinting of IGF2 and MEST in lung adenocarcinoma (2001)

Abstract Genomic imprinting is a parental origin–specific chromosomal modification that causes differential expression of maternal and paternal alleles of a gene. Accumulating evidence suggests that deregulation of imprinted genes, including loss of imprinting (LOI), plays a role in oncogenesis. In the present study, we investigated allelic expression of six imprinted genes in human lung adenocarcinomas as well as in matched normal lung tissue. Informative cases showing heterozygosity for the gene of interest were selected from 35 patients. LOI of the insulin‐like growth factor 2 gene ( IGF2 ) and mesoderm‐specific transcript ( MEST , also known as paternally expressed gene 1) was noted in 47% (seven of 15) and 85% (11 of 13) of informative cases, respectively. Monoallelic expression was maintained in all the matched normal tissues examined. LOI of IGF2 was seen more frequently in moderately to poorly differentiated adenocarcinomas. In contrast, H19 , small nuclear ribonucleoprotein–associated polypeptide N gene ( SNRPN ), necdin gene ( NDN ), and long QT intronic transcript 1 ( LIT1 ) exhibited consistent monoallelic expression in all the informative samples. These findings indicated that independent deregulation took place in imprinted genes and suggested that aberrant imprinting of IGF2 and MEST was involved in the development of lung adenocarcinoma. © 2001 Wiley‐Liss, Inc.
Microcapsule of type III strains of group B Streptococcus: production and morphology (1976)

The yield of purified type III polysaccharide of group B Streptococcus was significantly improved by modification of the growth medium. Culture of organisms in standard Todd-Hewitt broth resulted in acid accumulation during the exponential phase of growth and poor yield of type III polysaccharide when extracted from cells by washing with neutral buffer solution. By increasing the buffering capacity of the broth medium, acid accumulation was prevented, and the number of viable cells was increased at the stationary phase of growth. Further, by increasing the concentration of glucose in the buffered medium, the yield of type III polysaccharide was increased two to three times. Electron microscopic investigations of cells grown in the modified broth medium demonstrated a thicker microcapsule than was found in organisms grown in standard broth.
Tissue Hemoglobin O2 Saturation during Resuscitation of Traumatic Shock Monitored Using Near Infrared Spectrometry (2000)

Hemoglobin O2 saturation was monitored noninvasively and simultaneously in skeletal muscle and subcutaneous tissues as StO2 (%) by using a prototype NIR oximeter. Skeletal muscle StO2 tracked systemic O2 delivery during and after resuscitation. As a rapidly deployable, noninvasive monitor of peripheral tissue oxygenation and O2 delivery, skeletal muscle StO2 obtained using NIR spectrometry would be useful to guide resuscitation in the intensive care unit, to monitor resuscitation status in the operating room, and, potentially, in combination with indicators such as base deficit and lactate, to detect shock during initial assessment of the severe trauma patient in the emergency department.
Dataflow Predication (2006)

Predication facilitates high-bandwidth fetch and large static scheduling regions, but has typically been too complex to implement comprehensively in out-of-order micro architectures. This paper describes dataflow predication, which provides per-instruction predication in a dataflow ISA, low predication computation overheads similar to VLIW ISAs, and low complexity out-of-order issue. A two-bitfield in each instruction specifies whether an instruction is predicated, in which case, an arriving predicate token determines whether an instruction should execute. Dataflow predication incorporates three features that reduce predication overheads. First, dataflow predicate computation permits computation of compound predicates with virtually no overhead instructions. Second, early mispredication termination squashes in-flight instructions with false predicates at any time, eliminating the overhead of falsely predicated paths. Finally, implicit predication mitigates the fanout overhead of dataflow predicates by reducing the number of explicitly predicated instructions, by predicating only the heads of dependence chains. Dataflow predication also exposes new compiler optimizations - such as disjoint instruction merging and path-sensitive predicate removal - for increased performance of predicated code in an out-of-order design
The silicon solution [silicon photonics (2005)

As newer, faster microprocessors roll out, the copper connections that feed those processors within computers and servers will prove inadequate to handle huge amounts of data. To address this concern, this paper proposes the replacement of the copper with optical fiber and the electrons with photons. Called silicon photonics, this technology will allow manufacturers to build optical components using the same semiconductor equipment and methods they use now for ordinary integrated circuits. This would dramatically lower the cost of photonics while significantly improving performance.
The Structure of a Biologically Active Influenza Virus Ribonucleoprotein Complex (2009)

The influenza viruses contain a segmented, single-stranded RNA genome of negative polarity. Each RNA segment is encapsidated by the nucleoprotein and the polymerase complex into ribonucleoprotein particles (RNPs), which are responsible for virus transcription and replication. Despite their importance, information about the structure of these RNPs is scarce. We have determined the three-dimensional structure of a biologically active recombinant RNP by cryo-electron microscopy. The structure shows a nonameric nucleoprotein ring (at 12 Angstrom resolution) with two monomers connected to the polymerase complex (at 18 Angstrom resolution). Docking the atomic structures of the nucleoprotein and polymerase domains, as well as mutational analyses, has allowed us to define the interactions between the functional elements of the RNP and to propose the location of the viral RNA. Our results provide the first model for a functional negative-stranded RNA virus ribonucleoprotein complex. The structure reported here will serve as a framework to generate a quasi-atomic model of the molecular machine responsible for viral RNA synthesis and to test new models for virus RNA replication and transcription.
Bayesian Collaborative Denoising for Monte Carlo Rendering (2017)

Abstract The stochastic nature of Monte Carlo rendering algorithms inherently produces noisy images. Essentially, three approaches have been developed to solve this issue: improving the ray‐tracing strategies to reduce pixel variance, providing adaptive sampling by increasing the number of rays in regions needing so, and filtering the noisy image as a post‐process. Although the algorithms from the latter category introduce bias, they remain highly attractive as they quickly improve the visual quality of the images, are compatible with all sorts of rendering effects, have a low computational cost and, for some of them, avoid deep modifications of the rendering engine. In this paper, we build upon recent advances in both non‐local and collaborative filtering methods to propose a new efficient denoising operator for Monte Carlo rendering. Starting from the local statistics which emanate from the pixels sample distribution, we enrich the image with local covariance measures and introduce a nonlocal bayesian filter which is specifically designed to address the noise stemming from Monte Carlo rendering. The resulting algorithm only requires the rendering engine to provide for each pixel a histogram and a covariance matrix of its color samples. Compared to state‐of‐the‐art sample‐based methods, we obtain improved denoising results, especially in dark areas, with a large increase in speed and more robustness with respect to the main parameter of the algorithm. We provide a detailed mathematical exposition of our bayesian approach, discuss extensions to multiscale execution, adaptive sampling and animated scenes, and experimentally validate it on a collection of scenes.
Eighteen-Month-Old Fischer 344 Rats Fed a Spinach-Enriched Diet Show Improved Delay Classical Eyeblink Conditioning and Reduced Expression of Tumor Necrosis Factor α (TNFα) and TNFβ in the Cerebellum (2002)

Diets high in antioxidant properties are known to reverse some deficits in neuronal and cognitive function that occur in aging animals. Antioxidants are also known to reduce levels of proinflammatory factors in the CNS. We report here that 6 weeks of a spinach-enriched diet ameliorates deficits in cerebellar-dependent delay classical eyeblink learning and reduces the proinflammatory cytokines tumor necrosis factor alpha (TNFalpha) and TNFbeta in the cerebelli of eyeblink-trained animals. Eighteen-month-old Fischer 344 rats were given spinach-enriched lab chow or regular lab chow for 6 weeks. The rats were then given 6 d of 30 trials per day training using a 3 kHz tone conditioned stimulus and airpuff unconditioned stimulus. Rats were killed 3 weeks after eyeblink training. Cytokine expression was measured using RNase protection assay analysis in the eyeblink-trained animals and in a group of young control animals given regular lab chow diet. Old animals on the spinach-enriched lab chow diet learned delay eyeblink conditioning significantly faster than old animals on the regular diet. Cerebelli from older animals on the spinach-enriched diet had significantly less TNFalpha and TNFbeta than cerebelli from older animals on the control diet.
In vivo cleavage of transgene donors promotes nuclease‐mediated targeted integration (2012)

Targeted DNA integration is commonly used to eliminate position effects on transgene expression. Integration can be targeted to specific sites in the genome via both homology-based and homology-independent processes. Both pathways start the integration process with a site-specific break in the chromosome, typically from a zinc-finger nuclease (ZFN). We previously described an efficient homology-independent targeted integration technique that captures short (<100 bp) pieces of DNA at chromosomal breaks created by ZFNs. We show here that inclusion of a nuclease target site on the donor plasmid followed by in vivo nuclease cleavage of both the donor and the chromosome results in efficient integration of large, transgene-sized DNA molecules into the chromosomal double-strand break. Successful targeted integration via in vivo donor linearization is demonstrated at five distinct loci in two mammalian cell types, highlighting the generality of the approach. Finally, we show that CHO cells, a cell type recalcitrant to homology-based integration, are proficient at capture of in vivo-linearized transgene donors. Moreover, we demonstrate knockout of the hamster FUT8 gene via the simultaneous ZFN- or TALE nuclease-mediated integration of an antibody cassette. Our results enable efficient targeted transgene addition to cells and organisms that fare poorly with traditional homology-driven approaches.
The Interaction between Z-DNA and the Zab Domain of Double-stranded RNA Adenosine Deaminase Characterized Using Fusion Nucleases (1999)

Zab is a structurally defined protein domain that binds specifically to DNA in the Z conformation. It consists of amino acids 133-368 from the N terminus of human double-stranded RNA adenosine deaminase, which is implicated in RNA editing. Zab contains two motifs with related sequence, Zalpha and Zbeta. Zalpha alone is capable of binding Z-DNA with high affinity, whereas Zbeta alone has little DNA binding activity. Instead, Zbeta modulates Zalpha binding, resulting in increased sequence specificity for alternating (dCdG)n as compared with (dCdA/dTdG)n. This relative specificity has previously been demonstrated with short oligonucleotides. Here we demonstrate that Zab can also bind tightly to (dCdG)n stabilized in the Z form in supercoiled plasmids. Binding was assayed by monitoring cleavage of the plasmids using fusion nucleases, in which Z-DNA-binding peptides from the N terminus of double-stranded RNA adenosine deaminase are linked to the nuclease domain of FokI. A fusion nuclease containing Zalpha shows less sequence specificity, as well as less conformation specificity, than one containing Zab. Further, a construct in which Zbeta has been replaced in Zab with Zalpha, cleaves Z-DNA regions in supercoiled plasmids more efficiently than the wild type but with little sequence specificity. We conclude that in the Zab domain, both Zalpha and Zbeta contact DNA. Zalpha contributes contacts that produce conformation specificity but not sequence specificity. In contrast, Zbeta contributes weakly to binding affinity but discriminates between sequences of Z-DNAs.
Oxindoles. New, general method of synthesis (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOxindoles. New, general method of synthesisPaul G. Gassman and T. J. Van BergenCite this: J. Am. Chem. Soc. 1974, 96, 17, 5508–5512Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://doi.org/10.1021/ja00824a029RIGHTS & PERMISSIONSArticle Views1131Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (643 KB) Get e-Alerts Get e-Alerts
Google fails in search for cold fusion (2019)

Scientists at Google and several research institutes in North America have reopened what they call the “cold case” of cold fusion.