gebhart/patrepeval huggingface dataset:
Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine (1988)

A Shiga-like toxin type II variant (SLT-IIv) is produced by strains of Escherichia coli responsible for edema disease of swine and is antigenically related to Shiga-like toxin type II (SLT-II) of enterohemorrhagic E. coli. However, SLT-IIv is only active against Vero cells, whereas SLT-II is active against both Vero and HeLa cells. The structural genes for SLT-IIv were cloned from E. coli S1191, and the nucleotide sequence was determined and compared with those of other members of the Shiga toxin family. The A subunit genes for SLT-IIv and SLT-II were highly homologous (94%), whereas the B subunit genes were less homologous (79%). The SLT-IIv genes were more distantly related (55 to 60% overall homology) to the genes for Shiga toxin of Shigella dysenteriae type 1 and the nearly identical Shiga-like toxin type I (SLT-I) of enterohemorrhagic E. coli. (These toxins are referred to together as Shiga toxin/SLT-I.) The A subunit of SLT-IIv, like those of other members of this toxin family, had regions of homology with the plant lectin ricin. SLT-IIv did not bind to galactose-alpha 1-4-galactose conjugated to bovine serum albumin, which is an analog of the eucaryotic cell receptor for Shiga toxin/SLT-I and SLT-II. These findings support the hypothesis that SLT-IIv binds to a different cellular receptor than do other members of the Shiga toxin family but has a similar mode of intracellular action. The organization of the SLT-IIv operon was similar to that of other members of the Shiga toxin family. Iron did not suppress SLT-IIv or SLT-II production, in contrast with its effect on Shiga toxin/SLT-I. Therefore, the regulation of synthesis of SLT-IIv and SLT-II differs from that of Shiga toxin/SLT-I.
C reactive protein in the evaluation of febrile illness. (1986)

We studied prospectively 154 febrile children to determine the diagnostic value of the quantitative serum C reactive protein concentrations (CRP). Children with acute otitis media, acute tonsillitis, or treated with antibiotics during the two previous weeks and infants less than 2 months of age were excluded. Ninety seven children were from private paediatric practice and 57 were patients who had been admitted to hospital. The comparison group consisted of 75 children with confirmed bacterial infections whose CRP values were recorded retrospectively. In the study group 35 (23%) children had a confirmed viral infection, 92 (59%) had a probable viral infection as judged from the clinical picture and outcome of the illness, and 27 (18%) had a bacterial or probable bacterial infection. When the duration of the disease was more than 12 hours and the CRP value less than 20 mg/l, all children had a confirmed or probable viral infection. Nine children (one from the study group and eight from the comparison group) were found to have a septic infection and a CRP value of 20 mg/l or less. In all these cases, however, the duration of the symptoms was less than 12 hours. In addition CRP less than or equal to 20 mg/l was found in five (14%) children with urinary tract infection in the comparison group. CRP values of 20-40 mg/l were recorded in children with both viral and bacterial infections. A CRP value greater than or equal to 40 mg/l detected 79% of bacterial infections with 90% specificity. Our data show that determination of serum CRP concentrations is a valuable tool in evaluating children who have been ill for more than 12 hours.
The formation of palladium(II)– and platinum(II)–carbon bonds by proton abstraction from benzo[h]quinoline and 8-methylquinoline (1970)

The preparation of complexes containing palladium(II)– and platinum(II)–carbon bonds by proton abstraction from planar quinoline ligands, excludes a π-arene complex as a necessary intermediate during formation and presents the first examples of this reaction to include alkylprotons.
Photonics for switching and routing (2006)

The ongoing growth of data traffic from existing and new applications poses a challenge to the packet-switched network infrastructure. High-capacity transport can be achieved in such networks by using dense wavelength-division-multiplexed systems, and reconfigurable optical add-drop multiplexers allow the optical layer to provision wavelength-based circuits between routing nodes. However, construction of the high-capacity packet routers provides significant scaling issues due to complexity, interconnect, and thermal limits. In this paper we will not seek to cover all aspects of optical packet switching and routing but outline some of the challenges facing the construction of such future routers and describe the role photonics can have in overcoming some of these issues. We will discuss how photonics' primary role will be to provide interconnection between racks of electronic routing elements and describe how fast wavelength switching can provide a high-capacity distributed switch fabric that will allow these packet routers to scale to higher capacities. The fast wavelength switching can be seen as the packet analog of wavelength-based circuit switching of today's transparent optical networks
Nanobodies With In Vitro Neutralizing Activity Protect Mice Against H5N1 Influenza Virus Infection (2011)

Influenza A virus infections impose a recurrent and global disease burden. Current antivirals against influenza are not always effective. We assessed the protective potential of monovalent and bivalent Nanobodies (Ablynx) against challenge with this virus. These Nanobodies were derived from llamas and target H5N1 hemagglutinin. Intranasal administration of Nanobodies effectively controlled homologous influenza A virus replication. Administration of Nanobodies before challenge strongly reduced H5N1 virus replication in the lungs and protected mice from morbidity and mortality after a lethal challenge with H5N1 virus. The bivalent Nanobody was at least 60-fold more effective than the monovalent Nanobody in controlling virus replication. In addition, Nanobody therapy after challenge strongly reduced viral replication and significantly delayed time to death. Epitope mapping revealed that the VHH Nanobody binds to antigenic site B in H5 hemagglutinin. Because Nanobodies are small, stable, and simple to produce, they are a promising, novel therapeutic agent against influenza.
Inhibition of maturation and function of dendritic cells by intravenous immunoglobulin (2002)

Normal immunoglobulin G for therapeutic use (intravenous immunoglobulin [IVIg]) is used in an increasing number of immune-mediated conditions, including acute and chronic/relapsing autoimmune diseases, transplantation, and systemic inflammatory disorders. Several mutually nonexclusive mechanisms of action account for the immunoregulatory effects of IVIg. Although IVIg inhibits T-cell proliferation and T-cell cytokine production, it is unclear whether these effects are directly dependent on the effects of IVIg on T cells or they are dependent through the inhibition of antigen-presenting cell activity. Here, we examined the effects of IVIg on differentiation, maturation, and function of dendritic cells (DCs). We show that IVIg inhibits the differentiation and maturation of DCs in vitro and abrogates the capacity of mature DC to secrete interleukin-12 (IL-12) on activation while enhancing IL-10 production. IVIg-induced down-regulation of costimulatory molecules associated with modulation of cytokine secretion resulted in the inhibition of autoreactive and alloreactive T-cell activation and proliferation. Modulation of DC maturation and function by IVIg is of potential relevance to its immunomodulatory effects in controlling specific immune responses in autoimmune diseases, transplantation, and other immune-mediated conditions.
Sudden Cardiac Death: Exploring the Limits of Our Knowledge (2001)

Despite progress in epidemiology, clinical profiling, and interventions, sudden cardiac death remains a major clinical and public health problem. There remain important unresolved issues that are challenges for future progress. Among these are a better understanding of the magnitude of the problem and methods of profiling risk for individuals, the etiology and mechanisms of cardiac arrest in individuals with and without previously identified structural heart disease, clinical strategies for primary and secondary prevention of sudden cardiac death, and further development of community programs for improving cardiac arrest survival in the out-of-hospital environment. Each of these areas of endeavor and potential progress are reviewed and discussed.
Identification of Proteins from a Cell Wall Fraction of the Diatom Thalassiosira pseudonana (2005)

Diatoms are unicellular eucaryotic algae with cell walls containing silica, intricately and ornately structured on the nanometer scale. Overall silica structure is formed by expansion and molding of the membrane-bound silica deposition vesicle. Although molecular details of silica polymerization are being clarified, we have limited insight into molecular components of the silica deposition vesicle, particularly of membrane-associated proteins that may be involved in structure formation. To identify such proteins, we refined existing procedures to isolate an enriched cell wall fraction from the diatom Thalassiosira pseudonana, the first diatom with a sequenced genome. We applied tandem mass spectrometric analysis to this fraction, identifying 31 proteins for further evaluation. mRNA levels for genes encoding these proteins were monitored during synchronized progression through the cell cycle and compared with two previously identified silaffin genes (involved in silica polymerization) having distinct mRNA patterns that served as markers for cell wall formation. Of the 31 proteins identified, 10 had mRNA patterns that correlated with the silaffins, 13 had patterns that did not, and seven had patterns that correlated but also showed additional features. The possible involvements of these proteins in cell wall synthesis are discussed. In particular, glutamate acetyltransferase was identified, prompting an analysis of mRNA patterns for other genes in the polyamine biosynthesis pathway and identification of those induced during cell wall synthesis. Application of a specific enzymatic inhibitor for ornithine decarboxylase resulted in dramatic alteration of silica structure, confirming the involvement of polyamines and demonstrating that manipulation of proteins involved in cell wall synthesis can alter structure. To our knowledge, this is the first proteomic analysis of a diatom, and furthermore we identified new candidate genes involved in structure formation and directly demonstrated the involvement of one enzyme (and its gene) in the structure formation process.
Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), enhances anti-tumor effects of the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib in triple-negative breast cancer cells (2015)

Our findings suggest that expression of functional PTEN may serve as a biomarker for selecting TNBC patients that would favorably respond to a combination of olaparib with SAHA. This provides a strong rationale for treating TNBC patients with PTEN expression with a combination therapy consisting of olaparib and SAHA.
Ester Synthesis with Dicyclohexylcarbodiimide Improved by Acid Catalysts. (1979)

Die Ausb. der Veresterung der Carbonsauren (I) mit den Alkoholen (II) unter Anwendung von Dicyclohexylcarbodiimid (DCC) last sich wesentlich erhohen, wenn in Pyridin verestert wird und katalytische Mengen p-Toluolsulfonsaure zugesetzt werden.
Comparison of promoter suppression in avian and murine retrovirus vectors (1986)

Previously, we described "promoter suppression" in infectious retrovirus vectors with two genes and an internal promoter. Here, we examined several parameters of promoter suppression and found that the amount of suppression in an integrated retrovirus vector was dependent both on whether the vector was derived from spleen necrosis virus or murine leukemia virus and on which internal promoter was present in the vector. Murine leukemia virus vectors showed less suppression than analogous spleen necrosis virus vectors. Furthermore, the amount of suppression was not dependent on either the relative strengths of the promoters nor the distance between the promoters. Moreover, we found that in vectors in which one promoter was suppressed, there was an inverse correlation between the DNaseI sensitivity of the chromatin surrounding a promoter and the suppression of its expression.
Role of the Selective Contacts in the Performance of Lead Halide Perovskite Solar Cells (2014)

The effect of electron- and hole-selective contacts in the final cell performance of hybrid lead halide perovskite, CH3NH3PbI3, solar cells has been systematically analyzed by impedance spectroscopy. Complete cells with compact TiO2 and spiro-OMeTAD as electron- and hole-selective contacts have been compared with incomplete cells without one or both selective contacts to highlight the specific role of each contact. It has been described how selective contacts contribute to enhance the cell FF and how the hole-selective contact is mainly responsible for the high Voc in this kind of device. We have determined that the recombination rate is mainly governed by the selective contacts. This fact has important implication for the future optimization of perovskite solar cells. Finally, we have developed a method to analyze the results obtained, and it has been applied for three different electron-selecting materials: TiO2, ZnO, and CdS.
EnhanceNet: Single Image Super-Resolution Through Automated Texture Synthesis (2017)

Single image super-resolution is the task of inferring a high-resolution image from a single low-resolution input. Traditionally, the performance of algorithms for this task is measured using pixel-wise reconstruction measures such as peak signal-to-noise ratio (PSNR) which have been shown to correlate poorly with the human perception of image quality. As a result, algorithms minimizing these metrics tend to produce over-smoothed images that lack highfrequency textures and do not look natural despite yielding high PSNR values.,,We propose a novel application of automated texture synthesis in combination with a perceptual loss focusing on creating realistic textures rather than optimizing for a pixelaccurate reproduction of ground truth images during training. By using feed-forward fully convolutional neural networks in an adversarial training setting, we achieve a significant boost in image quality at high magnification ratios. Extensive experiments on a number of datasets show the effectiveness of our approach, yielding state-of-the-art results in both quantitative and qualitative benchmarks.
Physiological Levels of Pro- and Anti-Inflammatory Mediators in Cerebrospinal Fluid and Plasma: A Normative Study (2005)

Numerous recent studies have reported a significant inflammatory reaction in the brain and the systemic circulation following traumatic brain injury (TBI), infection, or neoplasm of the brain with a sequential release of pro- and anti-inflammatory mediators. Although there is growing knowledge and understanding of the mechanisms leading to the often poor outcome of these patients, only a limited database exists on the physiological expression of pro- and anti-inflammatory cytokines and molecules in plasma and particularly in cerebrospinal fluid (CSF). Therefore, we analyzed paired plasma/CSF samples of healthy human volunteers for the physiological concentrations of Interleukin (IL)-6, IL-8, IL-10, soluble TNF-receptors (sTNF-R) p55 and p75, soluble ICAM (sICAM), and soluble E-selectin (sE-selectin). A physiological release of IL-6, IL-8, IL-10, and sTNF-R p55 and p75 was detected in plasma and CSF. In contrast, sICAM and sE-selectin were only detectable in plasma. Pro- and anti-inflammatory mediators exhibited different concentration patterns in plasma and CSF, suggesting a pro-inflammatory predisposition in the central nervous system.
A human β-III-spectrin spinocerebellar ataxia type 5 mutation causes high-affinity F-actin binding (2016)

Spinocerebellar ataxia type 5 (SCA5) is a human neurodegenerative disease that stems from mutations in the SPTBN2 gene encoding the protein β-III-spectrin. Here we investigated the molecular consequence of a SCA5 missense mutation that results in a L253P substitution in the actin-binding domain (ABD) of β-III-spectrin. We report that the L253P substitution in the isolated β-III-spectrin ABD causes strikingly high F-actin binding affinity (Kd = 75.5 nM) compared to the weak F-actin binding affinity of the wild-type ABD (Kd = 75.8 μM). The mutation also causes decreased thermal stability (Tm = 44.6 °C vs 59.5 °C). Structural analyses indicate that leucine 253 is in a loop at the interface of the tandem calponin homology (CH) domains comprising the ABD. Leucine 253 is predicted to form hydrophobic contacts that bridge the CH domains. The decreased stability of the mutant indicates that these bridging interactions are probably disrupted, suggesting that the high F-actin binding affinity of the mutant is due to opening of the CH domain interface. These results support a fundamental role for leucine 253 in regulating opening of the CH domain interface and binding of the ABD to F-actin. This study indicates that high-affinity actin binding of L253P β-III-spectrin is a likely driver of neurodegeneration.
A Monolithic Photovoltaic-Photoelectrochemical Device for Hydrogen Production via Water Splitting (1998)

Direct water electrolysis was achieved with a novel, integrated, monolithic photoelectrochemical-photovoltaic design. This photoelectrochemical cell, which is voltage biased with an integrated photovoltaic device, splits water directly upon illumination; light is the only energy input. The hydrogen production efficiency of this system, based on the short-circuit current and the lower heating value of hydrogen, is 12.4 percent.
Large-Displacement Linear-Motion Compliant Mechanisms (2007)

Linear-motion compliant mechanisms have generally been developed for small displacement applications. The objective of the thesis is to provide a basis for improved large-displacement linear-motion compliant mechanisms (LLCMs). One of the challenges in developing large-displacement compliant mechanisms is the apparent performance tradeoff between displacement and off-axis stiffness. In order to facilitate the evaluation, comparison, and optimization of the performance of LLCMs, this work formulates and presents a set of metrics that evaluates displacement and off-axis stiffness. The metrics are non-dimensionalized and consist of the relevant characteristics that describe mechanism displacement, off-axis stiffness, actuation force, and size. Displacement is normalized by the footprint of the device. Transverse stiffness is normalized by a new performance characteristic called virtual axial stiffness. Torsional stiffness is normalized by a performance characteristic called the characteristic torque. Because large-displacement compliant mechanisms are often characterized by non-constant axial and off-axis stiffnesses, these normalized stiffness metrics are formulated to account for the variation of both axial and off-axis stiffness over the range of displacement. In pursuit of mechanisms with higher performance, this work also investigates the development of a new compliant mechanism element. It presents a pseudo-rigid-body model (PRBM) for rolling-contact compliant beams (RCC beams), a compliant element used in the RCC suspension. The loading conditions and boundary conditions for RCC beams can be simplified to an equivalent cantilever beam that has the same force-deflection characteristics as the RCC beam. Building on the PRBM for cantilever beams, this paper defines a model for the force-deflection relationship for RCC beams. Included in the definition of the RCC PRBM are the pseudo-rigid-body model parameters that determine the shape of the beam, the length of the corresponding pseudo-rigid-body links and the stiffness of the equivalent torsional spring. The behavior of the RCC beam is parameterized in terms of a single parameter defined as clearance, or the distance between the contact surfaces. The RCC beams exhibit a unique force-displacement curve where the force is inversely proportional to the clearance squared. The RCC suspension is modeled using the newly defined PRBM. The suspension exhibits unique performance, generating no resistance to axial motion while providing significant off-axis stiffness. The mechanism has a large range of travel and operates with frictionless motion due to the rolling-contact beams. In addition to functioning as a stand-alone linear-motion mechanism, the RCC suspension can be configured with other linear mechanisms in superposition to improve the off-axis stiffness of other mechanisms without affecting their axial resistance.
Frequency-domain spectral envelope estimation for low rate coding of speech (1999)

Estimation of the spectral envelope in the frequency domain allows to avoid some problems of linear prediction (LP) algorithms for voiced speech. We present a low complexity method of spectral envelope estimation from harmonics for low rate coding. The method consists in computing the harmonic amplitude spectrum using pitch-synchronous DFT with length depending on voicing, modifying this spectrum outside the telephone bandwidth to simplify modeling of the useful bandwidth and interpolating it by a frequency-domain low-pass filter. An all-pole model is then fitted to this modified smoothed version of the harmonic spectrum. The method was implemented on the harmonic-stochastic excitation (HSX) vocoder and the performance was compared with the LP algorithm similar to that used in the G.729 speech coding standard. A-B comparative tests show an important increase in perceptual quality.
LH in polycystic ovary syndrome: reproducibility and pulsatile secretion (1989)

Serum concentrations of LH are increased in polycystic ovary syndrome (PCOS). We have investigated two aspects of LH secretion which have not previously been reported: its reproducibility within individuals and the pattern of superimposed pulses of LH secretion. In nine patients with PCOS the mean concentration of LH was calculated from 24 blood samples taken at 15-min intervals for 6 h on two or three occasions over 1 year. Results showed differences in mean LH between subjects but reproducible concentrations within subjects over that period. It has been shown that LH is secreted in a complicated pattern of superimposed pulses which can be characterized by using the statistical methods of time-series analysis. To evaluate these pulse patterns of LH we studied nine patients with PCOS and compared the results with those of 12 normal women in the early follicular phase of the ovarian cycle. Blood samples were taken at either 5-min intervals for 6 h of 1-min intervals for 1 h. Pulses were detected in both groups at frequencies of about 1 h and 2 to 3 min. There was no significant difference in the pulse frequencies between the patients and controls but the amplitude of both groups of pulses was increased in the PCOS patients.
Protection Against Peroxynitrite-Dependent Tyrosine Nitration and α1-Antiproteinase Inactivation by Ascorbic Acid. A Comparison with other Biological Antioxidants (1996)

Peroxynitrite, formed by reaction of superoxide and nitric oxide, appears to be an important tissue-damaging species generated at sites of inflammation. In this paper, we compare the abilities of several biological antioxidants to protect against peroxynitrite-dependent inactivation of alpha 1-antiproteinase, and to inhibit tyrosine nitration upon addition of peroxynitrite. GSH and ascorbate protected efficiently in both systems. Uric acid inhibited tyrosine nitration but not alpha 1-antiproteinase inactivation. The possibility that ascorbic acid is an important scavenger of reactive nitrogen species in vivo is discussed.