gebhart/patrepeval huggingface dataset:
Bovine papillomavirus type 1 E1 and simian virus 40 large T antigen share regions of sequence similarity required for multiple functions (1997)

The full-length product of the bovine papillomavirus type 1 (BPV-1) E1 translational open reading frame is required for viral DNA replication in vivo and in vitro. E1 is a multifunctional protein whose properties include ATP binding, acting as an ATPase-dependent DNA helicase, DNA binding to the BPV-1 origin of viral DNA replication, and association with the E2 transcriptional transactivator, E2TA, a second viral protein involved in DNA replication. All of these properties are thought to be important for E1's role in replicating the viral genome. In addition BPV-1 E1 can inhibit activation of the viral P89 promoter by the BPV-1 E2TA. E1 has amino acid homology with eight regions of SV40 large tumor antigen (T-ag), a DNA helicase that is essential for the replication of the SV40 DNA genome. These eight regions of similarity lie within the domain of T-ag that confers DNA helicase activity. We created a series of missense mutations in BPV-1 E1 at codons 295, 344-345, 446, 464, 466, 497-498, 523, and 542, which encode amino acids of identity in seven of the eight regions of similarity between E1 and T-ag, and at codon 370. The activities of these mutant E1 genes were compared to wild-type E1 in multiple assays that measured DNA replication, inhibition of E2TA-dependent transcription, DNA binding, ATP binding, and protein expression. Based upon these analyses, the following conclusions were made: (i) at least five of the eight regions in E1 that are similar to regions in T-ag are functionally important in viral DNA replication; (ii) specific E1 missense mutants, themselves defective for supporting DNA replication, could act in trans to suppress the replication function of wild-type E1; (iii) certain regions of similarity with T-ag that are important for E1's ability to support DNA replication are not necessary for its capacity to inhibit E2TA-dependent transcription; and (iv) efficient DNA binding by E1 is not essential for E1 to inhibit E2TA-dependent transcription.
Any State or Event May Be a Reason for Which Somebody Does Something (2001)

Abstract Given that reasons for which people do things are states of affairs and events in the world, are there additional restrictions on what can be a reason for what? Many writers have claimed that there are, arguing that actions and the reasons for which they are done derive from particular systems of meaning. The pivot on which their argument turns is the notion of a constitutive rule, introduced by Rawls and taken up by writers like Charles Taylor and Searle; i.e., the notion of a rule such that without it the activity in question could not exist. The chapter argues, by contrast, that there are no constitutive rules, and that it is purely an empirical matter to figure out what is a reason for what action. This does not bar from reason status rules, customs, obligations, and similar things deemed to involve meanings.
Imaging and velocimetry of the human retinal circulation with color Doppler optical coherence tomography (2000)

Noninvasive monitoring of blood flow in retinal microcirculation may elucidate the progression and treatment of ocular disorders, including diabetic retinopathy, age-related macular degeneration, and glaucoma. Color Doppler optical coherence tomography (CDOCT) is a technique that allows simultaneous micrometer-scale resolution cross-sectional imaging of tissue microstructure and blood flow in living tissues. CDOCT is demonstrated for the first time in living human subjects for bidirectional blood-flow mapping of retinal vasculature.
The conversion of lignocellulosics to levulinic acid (2011)

Abstract Biomass represents an abundant and relatively low cost carbon resource that can be utilized to produce platform chemicals such as levulinic acid. Current processing technology limits the cost‐effective production of levulinic acid in commercial quantities from biomass. The key to improving the yield and efficiency of levulinic acid production from biomass lies in the ability to optimize and isolate the intermediate products at each step of the reaction pathway and reduce re‐polymerization and side reactions. New technologies (including the use of microwave irradiation and ionic liquids) and the development of highly selective catalysts would provide the necessary step change for the optimization of key reactions. A processing environment that allows the use of biphasic systems and/or continuous extraction of products would increase reaction rates, yields and product quality. This review outlines the chemistry of levulinic acid synthesis and discusses current and potential technologies for producing levulinic acid from lignocellulosics. © 2011 Society of Chemical Industry and John Wiley &amp; Sons, Ltd
EAHP news (2014)

Joan Peppard has been elected as the next President of the European Association of Hospital Pharmacists (EAHP), commencing a 3-year term of office from June 2015. She will now serve 1 year as President Elect during the final 12 months of Dr Roberto Frontini's Presidency of the Association.

The election took place at the General Assembly of EAHP in Sofia. The General Assembly is the annual occasion at which the 34 member countries of EAHP meet in person to determine policy, elect the Board, scrutinise the activities of the Association and conduct other decision making activity.

Joan, a chief pharmacist in a hospital in the Irish midlands, currently serves on the EAHP Board as Director of Professional Development and has twice been the head of the Hospital Pharmacists Association of Ireland in the position of President. Prior to joining the EAHP Board, Joan was for many years one of the lead delegates for Ireland within the EAHP annual General Assembly and played an active role in the conduct of EAHP educational and research activities at the national level.

Speaking after her election, Joan Peppard, President-Elect of EAHP said: “It is with a sense of great humility that I begin to take on the primary leadership role of the EAHP, knowing that under the stewardship of predecessors a real momentum of energy and experience has been established to make EAHP the driver for hospital pharmacy practice improvement across Europe that it is today. With the new European Statements of Hospital Pharmacy recently agreed …
The reappearance of dengue-3 and a subsequent dengue-4 and dengue-1 epidemic in Puerto Rico in 1998. (2002)

In January 1998, dengue-3 (DEN)-3 (group III genotype) was detected in Puerto Rico after an absence of 20 years. Public health officials intensified education efforts to promote community participation in dengue control. Virologic surveillance revealed an unexpected paradox: DEN-4 and DEN-1 produced a large epidemic overlaying the DEN-3 epidemic. In 1998 there were 17,000 reported cases of dengue (4.8/1,000 persons), and among all virus isolations (n = 960), DEN-4 (419, 43.6%), DEN-1 (337, 35.1%), and DEN-2 (143, 14.9%) were detected much more frequently than DEN-3 (61, 6%). Age group-specific attack rates were highest for persons 10-19 years old, followed by infants less than a year of age. Nineteen fatal cases (median = 37 years old, range = 8 months to 90 years) had a positive laboratory diagnosis of dengue. Among DEN-3 cases no fatalities were documented, 50 were hospitalized, and 10 of 48 (21%) fulfilled the criteria for dengue hemorrhagic fever (four had primary infections and six had secondary infections). During 1999, DEN-3 became the predominant serotype isolated (182 of 310 isolations, 59%). The reappearance of DEN-3 and its subsequent circulation from 1999 to 2001 produced no changes in dengue incidence that could have been detected in the absence of virologic surveillance.
Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages (2013)

In the treatment of bacterial infections, antibiotics have proven to be very effective, but the way in which antibiotics are dosed can create a lag time between the administration of the drug and its absorption at the site of insult. The time it takes an antibiotic to reach therapeutic levels can often be significantly increased if the vascular system is compromized. Bacteria can multiply pending the delivery of the drug, therefore, developing treatments that can inhibit the inflammatory response while waiting for antibiotics to take effect could help prevent medical conditions such as septic shock. The aim of this study was to examine the effect of a pulsed electromagnetic field on the production of inflammatory markers tumor necrosis factor (TNF), transcription factor nuclear factor kappa B (NFkB), and the expression of the A20 (tumor necrosis factor-alpha-induced protein 3), in an inflamed-cell model. Lipopolysaccharide-challenged cells were exposed to a pulsed electromagnetic field at various frequencies in order to determine which, if any, frequency would affect the TNF-NFkB-A20 inflammatory response pathway. Our study revealed that cells continuously exposed to a pulsed electromagnetic field at 5 Hz demonstrated significant changes in the downregulation of TNF-α and NFkB and also showed a trend in the down regulation of A20, as compared with controls. This treatment could be beneficial in modulating the immune response, in the presence of infection.
Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-κB activation (2007)

Tissue damage is usually followed by healing, as both differentiated and stem cells migrate to replace dead or damaged cells. Mesoangioblasts (vessel-associated stem cells that can repair muscles) and fibroblasts migrate toward soluble factors released by damaged tissue. Two such factors are high mobility group box 1 (HMGB1), a nuclear protein that is released by cells undergoing unscheduled death (necrosis) but not by apoptotic cells, and stromal derived factor (SDF)-1/CXCL12. We find that HMGB1 activates the canonical nuclear factor kappaB (NF-kappaB) pathway via extracellular signal-regulated kinase phosphorylation. NF-kappaB signaling is necessary for chemotaxis toward HMGB1 and SDF-1/CXCL12, but not toward growth factor platelet-derived growth factor, formyl-met-leu-phe (a peptide that mimics bacterial invasion), or the archetypal NF-kappaB-activating signal tumor necrosis factor alpha. In dystrophic mice, mesoangioblasts injected into the general circulation ingress inefficiently into muscles if their NF-kappaB signaling pathway is disabled. These findings suggest that NF-kappaB signaling controls tissue regeneration in addition to early events in inflammation.
Conducting redox polymers: investigations of polythiophene-Ru(bpy)3n+ hybrid materials (1999)

A series of thiophene-appended Ru II (bpy) 3 derivatives, Ru(1) 3 , Ru(2) 3 , Ru(3) 3 , Ru(bpy) 2 (1), and Ru(bpy) 2 (2), and their resulting polymers have been synthesized and characterized. The bpy ligands 5,5′-bis(5-(2,2′-bithienyl))-2,2′-bipyridine, 1, 4,4′-bis(5-(2,2′-bithienyl))-2,2′-bipyridine, 2, and 4-(5-(2,2′-bithienyl))-2,2′-bipyridine, 3, all contain electrochemically polymerizable bithienyl moieties. The monomers Ru(2) 3 , Ru(3) 3 , Ru(bpy) 2 (1) and Ru(bpy) 2 (2) display spectroscopic features that are similar to the ligand-based and MLCT bands found for Ru(bpy) 3 . The cyclic voltammograms of all of these polymers display both metal-centered and thiophene-based electroactivity. High redox conductivity was found in poly(Ru(2) 3 ) and poly(Ru(3) 3 ) for both the thiophene-based oxidation and metal-based reduction processes. These results indicate that the polymers display charge localization for both the metal complexes as well as the tetrathienyl connecting units. The degree of interconnection (number of linkages) as well as the substitution pattern were found to control the conductivity of these polymers. The highest conductivity (3.3×10 –3 S cm –1 ) was found for poly(Ru(2) 3 ), which is able to have up to 6 linkages with other ruthenium complexes as well as possessing a 4,4′-substitution pattern that allows effective orbital overlap of the conjugated polymer backbone with the ruthenium centers.
Immune hemolysis following ABO-mismatched stem cell or solid organ transplantation (2007)

ABO-mismatched donors are commonly used for transplantation, and immune hemolysis remains the main complication. Clinicians must be vigilant in order to recognize hemolysis and implement appropriate therapy.
A method for reducing the time required to match protein sequences with tandem mass spectra (2003)

Abstract An algorithm for reducing the time necessary to match a large set of peptide tandem mass spectra with a list of protein sequences is described. This algorithm breaks the process into multiple steps. A rapid survey step identifies all protein sequences that are reasonable candidates for a match with a set of tandem mass spectra. These candidates are then used as models, which are refined by detailed analysis of the set of tandem mass spectra for evidence of incomplete enzymatic hydrolysis, non‐specific hydrolysis and chemical modifications of amino acid residues resulting from either post‐translational modifications or sample handling. Compared with current one‐step methods for matching proteins to mass spectra, this multiple‐step method can decrease the time required for the calculation by several orders of magnitude. Copyright © 2003 John Wiley &amp; Sons, Ltd.
Effect of Deuteration of N—CH3Group on Potency and Enzymatic N-Demethylation of Morphine (1961)

Substitution of deuterium for the N-methyl hydrogens of morphine produced a significant reduction in the potency and lethality of morphine in mice regardless of the route of administration. There was no effect on the time of onset, maximal effect, or duration of action. N-demethylation by rat liver microsomal enzymes was characterized by a smaller reaction rate constant, a higher energy of activation, and a larger Michaelis constant with respect to the deuterated morphine. These findings indicated that deuteration of the N-methyl group of morphine not only caused reduction in potency, but also a reduction in the rate of oxidative N-demethylation, and a distinct weakening of the binding to the enzyme active centers.
Evolution map from TD-SCDMA to FuTURE B3G TDD (2006)

The rapid growth of mobile applications in recent years has created a need for wideband wireless communication. The only predictable trend is that data-rate and QoS requirements will increase rapidly. This demand is pushing time-division-synchronous code-division multiple access (TD-SCDMA) to evolve in order to provide higher and higher data rates. This article presents the evolution map from TD-SCDMA to future terrestrial universal radio environment (FuTURE) TDD in China. The evolution includes four phases: low chip rate (LCR), high-speed downlink/uplink packet access (HSxPA)/TD-SCDMA EV 1x, long-term evolution (LTE) TDD, and FuTURE beyond 3G TDD. The main features of each phase are described in detail. By introducing the new technologies into the system step by step, for example, multiple input and multiple output (MIMO), orthogonal frequency-division multiplexing (OFDM), TD-SCDMA system can evolve to FuTURE B3G TDD smoothly, and provide high-data-rate services with low cost, low latency, and improved coverage and capacity.
The Selective Estrogen Receptor Modulator Arzoxifene and the Rexinoid LG100268 Cooperate to Promote Transforming Growth Factor β-Dependent Apoptosis in Breast Cancer (2004)

We show that the selective estrogen receptor modulator arzoxifene (Arz) and the rexinoid LG100268 (268) synergize to promote apoptosis in a rat model of estrogen receptor-positive breast carcinoma and in estrogen receptor-positive human breast cancer cells in culture. We also show that it is not necessary to administer Arz and 268 continuously during tumor progression to prevent cancer in the rat model because dosing of these drugs in combination for relatively short periods, each followed by drug-free rests, is highly effective. This new approach to chemoprevention uses high doses of drugs that are too toxic for long-term administration. However, when given for short periods, the agents are nontoxic and still induce apoptosis in breast cancer cells. We also show that the ability of the two drugs to induce apoptosis is the combined result of induction of transforming growth factor beta by Arz, together with inhibition of the prosurvival nuclear factor kappaB and phosphatidylinositol 3' kinase signaling pathways by 268. The new protocol we have developed for chemoprevention allows the efficacious and safe administration of 268 and Arz, and these agents now should be considered for clinical use.
Clinical Score for Predicting Recurrence After Hepatic Resection for Metastatic Colorectal Cancer (1999)

Resection of hepatic colorectal metastases may produce long-term survival and cure. Long-term outcome can be predicted from five criteria that are readily available for all patients considered for resection. Patients with up to two criteria can have a favorable outcome. Patients with three, four, or five criteria should be considered for experimental adjuvant trials. Studies of preoperative staging techniques or of adjuvant therapies should consider using such a score for stratification of patients.
ChemInform Abstract: SYNTHESIS AND STRUCTURE‐ACTIVITY RELATIONSHIPS OF 1‐SUBSTITUTED‐AMINOMETHYL‐3‐PHENYL/METHYL‐1,3‐DIHYDROISOBENZOFURANS AND 4‐SUBSTITUTED‐AMINO‐1‐PHENYL/METHYLISOCHROMANS ‐ A NEW CLASS OF ANTIHISTAMINICS (1985)

Abstract Die aus den Ketosäuren (I) zugänglichen Isochromanone (II) werden in die Bromderivate (III) übergeführt, aus denen einerseits über die Carbonsäuren (IV) die Amide (V) und andererseits die Amino‐isochromanone (VII) hergestellt werden.
Glomerular Hyperfiltration and Renal Progression in Children with Autosomal Dominant Polycystic Kidney Disease (2011)

This study revealed that occurrence of GH in ADPKD children is associated with a significantly faster decline in renal function and higher rate of kidney enlargement over time. GH combined with the increased renal volume may therefore be used as an early marker for a more severe progression of ADPKD in children.
Ligase chain reaction for detection of Neisseria gonorrhoeae in urogenital swabs (1995)

The ligase chain reaction (LCR) is an in vitro nucleic acid amplification technique that exponentially amplifies targeted DNA sequences. In a multicenter study, we evaluated the use of a 4-h LCR-based assay for the diagnosis of Neisseria gonorrhoeae infection of the cervix and male urethra. The LCR results were compared with those of culture for N. gonorrhoeae by using selective media. This assay amplifies target sequences within the N. gonorrhoeae opacity gene. Discordant LCR-positive and culture-negative specimens were further evaluated by testing by another LCR assay which used N. gonorrhoeae-specific pilin probe sets. A total of 1,539 female endocervical specimens and 808 male urethral swab specimens were evaluated in the study. An expanded "gold standard" was defined to include all culture-positive as well as culture-negative, confirmed LCR-positive specimens. After resolution of discrepant samples, the sensitivities of the N. gonorrhoeae LCR assays for the female and male specimens were 97.3 and 98.5%, respectively, with specificities of 99.6 and 99.8%, respectively. Resolved culture sensitivities were 83.9 and 96.5% for the female and male specimens, respectively. The LCR assay for gonorrhea is a rapid, highly sensitive nonculture method for detecting gonococcal infection of the cervix and male urethra.
HISTODIFFERENTIATION OF HAIR FOLLICLES IN GRAFTING OF CELL AGGREGATES OBTAINED BY ROTATION CULTURE OF EMBRYONIC RAT SKIN (1998)

We have previously reported reconstruction of hair follicles from a single cell suspension of rat fetal upper lip by a two-step culture method consisting of rotation and flotation cultures. Rotation sorted out the cells and flotation facilitated histodifferentiation. In the present study, we added grafting procedures to the previous method to see whether cell aggregates obtained this way were graftable, and whether the grafting promoted histodifferentiation. The aggregates before and after flotation were grafted, and differentiation of hair follicles comparable to those in vivo was confirmed 10 days after grafting. There was no difference in the degree of differentiation between the two kinds of grafts. The grafting procedure therefore resulted in an appreciable increase in histodifferentiation even when aggregates obtained after flotation were grafted.
High-power visible laser effect on a Boston Micromachines' MEMS deformable mirror (2010)

Continuous-facesheet and segmented Boston Micromachines Corporations' (BMC) Micro-Electrical Mechanical Systems (MEMS) Deformable Mirrors (DM) have been tested for their response to high-power visible-wavelength laser light. The deformable mirrors, coated with either protected silver or bare aluminum, were subjected to a maximum of 2 Watt laser-light at a wavelength of 532 nanometers. The laser light was incident on a ~ 3.5&times;3.5 cm area for time periods from minutes to 7 continuous hours. Spot heating from the laser-light is measured to induce a local bulge in the surface of each DM. For the aluminum-coated continuous facesheet DM, the induced spot heating changes the surface figure by 16 nm rms. The silver-coated continuous-facesheet and segmented (spatial light modulator) DMs experience a 6 and 8 nm surface rms change in surface quality with the laser at 2 Watts. For spatial frequencies less than the actuator spacing (300 mm), the laser induced surface bulge is shown to be removable, as the DMs continued to be fully functional during and after their exposure. Over the full 10 mm aperture one could expect the same results with a 15 Watt laser guide star (LGS). These results are very promising for use of the MEMS DM to pre-correct the outgoing laser light in the Laboratory for Adaptive Optics' (LAO) laser uplink application.
An Experimental and Molecular‐Modeling Study of the Binding of Linked Sulfated Tetracyclitols to FGF‐1 and FGF‐2 (2005)

The experimental binding affinities of a series of linked sulfated tetracyclitols [Cyc2N-R-NCyc2, where Cyc = C6H6(OSO3Na)3 and R = (CH2)n (n = 2-10), p-xylyl or (C2H4)2-Ncyc] for the fibroblast growth factors FGF-1 and FGF-2 have been measured by using a surface plasmon resonance assay. The KD values range from 7.0 nM to 1.1 microM for the alkyl-linked ligands. The binding affinity is independent of the flexibility of the linker, as replacement of the alkyl linker with a rigid p-xylyl group did not affect the KD. Calculations suggest that binding modes for the p-xylyl-linked ligand are similar to those calculated for the flexible alkyl-linked tetracyclitols. The possible formation of cross-linked FGF:cyclitol complexes was examined by determining KD values at increasing protein concentrations. No changes in KD were observed; this suggesting that only 1:1 complexes are formed under these assay conditions. Monte Carlo multiple-minima calculations of low-energy conformers of the FGF-bound ligands showed that all of the sulfated tetracyclitol ligands can bind effectively in the heparan sulfate-binding sites of FGF-1 and FGF-2. Binding affinities of these complexes were estimated by the Linear Interaction Energy (LIE) method to within a root-mean-square deviation of 1 kcal mol(-1) of the observed values. The effect of incorporating cations to balance the overall charge of the complexes during the LIE calculations was also explored.
Telomere Gap Between Granulocytes and Lymphocytes Is a Determinant for Hematopoetic Progenitor Cell Impairment in Patients With Previous Myocardial Infarction (2008)

In patients with CAD, telomere shortening of BMCs is dependent on both age and the extent of CAD and correlates with bone marrow cell functionality.