gebhart/patrepeval huggingface dataset:
Immunomagnetic enrichment of disseminated epithelial tumor cells from peripheral blood by MACS. (1998)

Disseminated epithelial tumor cells have been detected in the bone marrow and blood of cancer patients by means of immunocytochemical or immunofluorescent staining of cytocentrifuge slides, multiparameter flow cytometry, and reverse transcriptase-polymerase chain reaction. However, it is hardly possible using such methods to detect tumor cells at a frequency below 10(-6). To increase the sensitivity of these detection techniques we have developed a new technology for the enrichment of disseminated epithelial tumor cells from hematopoietic cell samples by high-gradient magnetic cell sorting (MACS). Cells are permeabilized and fixed and carcinoma cells are magnetically labeled specifically with an anti-cytokeratin 8 monoclonal antibody (mAb) directly conjugated to superparamagnetic microbeads. Magnetically labeled cells are enriched on high-gradient magnetic columns. Tumor cells are detected in the enriched cell fraction by flow cytometry, fluorescence microscopy, or immunocytochemisty. In this study we demonstrated the method using a model system in which five to 5,000 cells from a breast cancer cell line were seeded into blood cell samples from a healthy donor containing 1.2 x 10(8) leukocytes. Tumor cells were 10,477+/-4242 (n=25)-fold magnetically enriched, and 57.7%+/-16.9% (n=33) of the initially seeded tumor cells were recovered. Applying the method to 20-40 mL blood samples from patients with advanced carcinomas of the breast, prostate, colon, rectum, or lung, we were able to detect between one and 6.8 x 10(4) cytokeratin-expressing tumor cells in 21 of 34 patients. This corresponds to frequencies of tumor cells between 6.8 x 10(-9) and 1.1 x 10(-3) among nucleated cells in the original sample. Enriched tumor cells were further analyzed for expression of tissue-specific and prognostic markers such as breast mucin glycoproteins, erbB2, and CD44v6 for additional characterization and to confirm their tumor origin. The technique described could become a valuable tool for the quantification and molecular characterization of metastatic carcinoma cells in hematopoietic tissue, and may ultimately prove useful in the diagnosis, prognosis, and monitoring of patients with carcinoma.
A Comparison of Job-facet Satisfaction and Commitment Determinants of Turnover Intention (1995)

This study compares the effects of job facet satisfaction and commitment dimensions on employee turnover intentions. It also examines whether there is sufficient support for a paradigm shift from job facet satisfaction to commitment dimensions in turnover research. From a survey of 259 employees in Hong Kong, results indicate that satisfaction with the identified job facets accounts for a larger amount of variance than commitment dimensions in turnover intention. In this context, replacing job facet satisfaction with commitment dimensions may be empirically untenable. It also appears that commitment dimensions may mediate the relationships between certain facets of job satisfaction and turnover intention. Further investigation is required to better appreciate the complexity of the satisfaction-commitment-turnover linkage.
In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents (2009)

A powerful strategy for targeted drug discovery is the use of bacteriophage (phage) display technology for identification of peptide-based tumor targeting agents. Peptide pharmaceuticals may possess clinically desirable properties because of their rapid blood clearance, non-immunogenic nature, and ease of synthesis. Phage display has identified hundreds of different peptide sequences that bind a desired target in vitro. Regrettably, few of these peptides offer good targeting efficacy in vivo. One reason for this is the synthesized peptide may not retain its optimal activity outside the microenvironment of the phage. Another possible explanation is that traditionally, phage selections are performed in vitro outside the complicated milieu of a living animal. Given these shortcomings, we have developed methods to select phage peptide display libraries in living mice, to identify, a priori, phage (and corresponding synthesized peptides) with ideal tumor-targeting propensity.
'Ballistaria' in First to Mid-Third Century Britain: A Reappraisal (1984)

The Cawthorn encampments in North Yorkshire (FIG. I) have found wide publication as ‘practice forts’ with attendant construction camps following their investigation by the late Sir Ian Richmond in the 1920s. The fundamental reason for his interpretation of Cawthorn as the site of military manoeuvres rather than as a normal fort site was its alleged unsuitability for permanent occupation coupled with apparently unfinished defences. However, it has recently been postulated that Camp D, the most regularly laid-out of the four, represents an auxiliary fort. This suggestion does not contravene the evidence; indeed, Camps A and B (the secondary enlargement of A) need no more be connected with manoeuvres than Camp D. One of the major objections to their being regular forts is based upon the presence of ballistaria. Richmond used these as proof that legionaries rather than auxiliaries were involved at Cawthorn; certainly, it seems that the auxilia were not equipped with artillery in the first and second centuries a.d., SO that artillery-platforms at this date should indicate the presence of legionaries. The conclusion that the encampments are not regular forts but practice-works is reinforced by the fact that none of the ballistaria is situated at a strongly defensive position. Thus, the application of Richmond's hypothesis to Camps A and B appears to be sound. Nevertheless, it represents the only evidence that the Romans ever built forts specifically for practice. Although practice camps are abundant both in the archaeological record and in the literary sources, nowhere are practice forts attested.
Receptor tyrosine phosphatase R-PTP-kappa mediates homophilic binding. (1994)

Receptor tyrosine phosphatases (R-PTPases) feature PTPase domains in the context of a receptor-like transmembrane topology. The R-PTPase R-PTP-kappa displays an extracellular domain composed of fibronectin type III motifs, a single immunoglobulin domain, as well as a recently defined MAM domain (Y.-P. Jiang, H. Wang, P. D'Eustachio, J.M. Musacchio, J. Schlessinger, and J. Sap, Mol. Cell. Biol. 13:2942-2951, 1993). We report here that R-PTP-kappa can mediate homophilic intercellular interaction. Inducible expression of the R-PTP-kappa protein in heterologous cells results in formation of stable cellular aggregates strictly consisting of R-PTP-kappa-expressing cells. Moreover, the purified extracellular domain of R-PTP-kappa functions as a substrate for adhesion by cells expressing R-PTP-kappa and induces aggregation of coated synthetic beads. R-PTP-kappa-mediated intercellular adhesion does not require PTPase activity or posttranslational proteolytic cleavage of the R-PTP-kappa protein and is calcium independent. The results suggest that R-PTPases may provide a link between cell-cell contact and cellular signaling events involving tyrosine phosphorylation.
Bile Acid-Derived Molecular Tweezers:  Study of Solvent Effects in Binding, and Determination of Thermodynamic Parameters by an Extraction-Based Protocol (2000)

A family of bile acid-based molecular tweezers (1-3) were synthesized, and their binding affinities with picric acid in different solvents were evaluated using a simple extraction-based protocol. The binding affinities increased in nonpolar solvents. The size of the solvent molecule did not affect the binding constant. Thermodynamic parameters for the binding of picric acid in CCl(4) were also determined by this method. Binding constants of these tweezers with trinitrofluorenone in CDCl(3) were determined by NMR titration.
Highly resolved Diffusion Tensor Imaging of the spinal cord at 3.0 Tesla (2008)

Purpose: Despite the promising results in the brain1, the application of the diffusion tensor imaging (DTI) techniques to the spinal cord is still hampered by limited signal-to-noise ratio (SNR) and spatial resolution2. High-field MRI allows for increasing SNR3. However, image blurring may occur in gradient-echo based sequences due to the faster T2* signal decay at higher field strength. In this study the feasibility of highly- resolved DTI of the spinal cord at 3.0T was tested.
Bispecific T‐cell engagers for cancer immunotherapy (2014)

Bispecific T-cell engagers (BiTEs) are a new class of immunotherapeutic molecules intended for the treatment of cancer. These molecules enhance the patient's immune response to tumors by retargeting T cells to tumor cells. BiTEs are constructed of two single-chain variable fragments (scFv) connected in tandem by a flexible linker. One scFv binds to a T-cell-specific molecule, usually CD3, whereas the second scFv binds to a tumor-associated antigen. This structure and specificity allows a BiTE to physically link a T cell to a tumor cell, ultimately stimulating T-cell activation, tumor killing and cytokine production. BiTEs have been developed, which target several tumor-associated antigens, for a variety of both hematological and solid tumors. Several BiTEs are currently in clinical trials for their therapeutic efficacy and safety. This review examines the salient structural and functional features of BiTEs, as well as the current state of their clinical and preclinical development.
PIR with Compressed Queries and Amortized Query Processing (2018)

Private information retrieval (PIR) is a key building block in many privacy-preserving systems. Unfortunately, existing constructions remain very expensive. This paper introduces two techniques that make the computational variant of PIR (CPIR) more efficient in practice. The first technique targets a recent class of CPU-efficient CPIR protocols where the query sent by the client contains a number of ciphertexts proportional to the size of the database. We show how to compresses this query, achieving size reductions of up to 274X. The second technique is a new data encoding called probabilistic batch codes (PBCs). We use PBCs to build a multi query PIR scheme that allows the server to amortize its computational cost when processing a batch of requests from the same client. This technique achieves up to 40× speedup over processing queries one at a time, and is significantly more efficient than related encodings. We apply our techniques to the Pung private communication system, which relies on a custom multi-query CPIR protocol for its privacy guarantees. By porting our techniques to Pung, we find that we can simultaneously reduce network costs by 36× and increase throughput by 3X.
Characterization of gyrA, gyrB, grlA and grlB mutations in fluoroquinolone-resistant clinical isolates of Staphylococcus aureus (1998)

The distribution of fluoroquinolone resistance-associated point mutations in genes encoding the subunits of DNA gyrase and DNA topoisomerase i.v. was examined in 110 clinical isolates of Staphylococcus aureus. Point mutations were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis and mutations were further characterized by sequencing of PCR products. Mutations at Ser84 of GyrA were widely distributed among isolates exhibiting various degrees of fluoroquinolone resistance, and border zones between mutant and non-mutant strains based on drug susceptibility were generally distinct. Mutations at Ser80 of GrlA were also widely distributed, but border zones between mutant and non-mutant isolates were in this case less distinct and several GrlA mutants were highly susceptible to sparfloxacin and tosufloxacin. Only two gyrB mutants and one grlB mutant were observed among the isolates: all contained a previously unreported mutation. GyrA and grlA mutations thus appear to impart high levels of fluoroquinolone resistance in many S. aureus clinical isolates.
Granulocytic Ehrlichiosis in Dogs from North Carolina and Virginia (1998)

Medical records of 3 dogs from North Carolina and 3 dogs from Virginia with ehrlichial morulae in circulating neutrophils were studied retrospectively. Two clinically distinct disease syndromes, including chronic, moderate to severe anemia (n = 3) and polyarthritis (n = 2) were associated with canine granulocytic ehrlichiosis (CGE) in these dogs. One dog was clinically healthy, and abnormalities were not detected during physical examination. Clinical signs were nonspecific and included fever, lethargy, anorexia, vomiting, and diarrhea. The most frequent laboratory abnormalities were normocytic normochromic nonregenerative anemia, moderate thrombocytopenia with large platelets, lymphopenia, and eosinopenia. Considerable variability was found in the serologic responses to Ehrlichia equi, Ehrlichia canis, and Ehrlichia chaffeensis antigens among the 5 dogs for which stored sera were available for indirect fluorescent antibody testing. Polymerase chain reaction amplification and sequencing of portions of the 16S rRNA gene from blood (collected in ethylenediaminetetraacetic acid) of 1 severely anemic dog (dog 3) and 1 polyarthritic dog (dog 4) resulted in DNA sequences nearly identical to the GenBank accessions for Ehrlichia ewingii. The DNA sequence from a 3rd dog (dog 5) was most similar to that of E. canis. Serologic or molecular results support the possibility of E. ewingii, E. equi, and E. canis coinfection or serologic cross-reactivity among canine granulocytic and monocytic Ehrlichia species in dogs from North Carolina and Virginia. Variability in response to tetracycline or doxycycline treatment was noted in these dogs, with more rapid resolution of signs in dogs with polyarthritis. We report the 1st cases of CGE in dogs from North Carolina and Virginia, including recognition of CGE in a healthy dog.
High-rate entanglement source via two-photon emission from semiconductor quantum wells (2007)

We propose a compact high-intensity room-temperature source of entangled photons based on the efficient second-order process of two-photon spontaneous emission from electrically pumped semiconductor quantum wells in a photonic microcavity. Two-photon emission rate in room-temperature semiconductor devices is determined solely by the carrier density, regardless of the residual one-photon emission. The microcavity selects two-photon emission for a specific signal and idler wavelengths and at a preferred direction without modifying the overall rate. Pair-generation rate in $\mathrm{Ga}\mathrm{As}∕\mathrm{Al}\mathrm{Ga}\mathrm{As}$ quantum well structure is estimated using a 14-band model to be 3 orders of magnitude higher than for traditional broadband parametric down-conversion sources.
Lipophilisation of hydrophilic compounds. Consequences on transepidermal and intestinal transport of trospium chloride. (1987)

Lipophilic ion pairs of trospium chloride (Spasmex) were synthesized and the transfer rates of the parent drug and the derivatives across everted rat intestine and human abdominal epidermis were measured. It was evaluated whether there is a correlation between membrane permeation and hydrophilic/lipophilic balance. With some ion pairs of trospium, enhanced transport rates in both models could be found.
Determination of catecholamines and their 3‐O‐methyl metabolites in mouse plasma (2001)

The determination of catecholamines and their 3-O-methyl metabolites in a single mouse plasma is necessary to understand the role of the sympathetic nervous activity, while the inactivation of catecholamines by catechol-O-methyltransferase indicates the activity of blood pressure regulation in animals. Here we report the basal catecholamines and their 3-O-methyl metabolite concentrations obtained from 15 microL of mouse plasma utilizing semi-microcolumn high-performance liquid chromatography (HPLC)-peroxyoxalate chemiluminescence detection system. The concentrations were 6.63 +/- 1.37 pmol/mL plasma, 0.49 +/- 0.10 pmol/mL plasma, 5.25 +/- 2.30 pmol/mL plasma, 3.23 +/- 0.84 pmol/mL plasma, 0.44 +/- 0.11 pmol/mL plasma, and 3.39 +/- 1.67 pmol/mL plasma for norepinephrine, epinephrine, dopamine, normetanephrine, metanephrine and 3-methoxytyramine, respectively (n = 5-7). Further, when blood pressure was reduced by minoxidil, plasma catecholamines were found to be significantly increased by the baroreflex-mediated response in mouse.
Posterior atlantoaxial facet screw fixation in rheumatoid arthritis (1998)

Posterior atlantoaxial transarticular screw fixation provides a good surgical alternative for the management of patients with rheumatoid atlantoaxial instability. This technique provides immediate three-point rigid fixation of the C1-2 region, thus obviating the need for halo vest immobilization in most cases.
Multiwavelength survivable ring network architectures (2002)

A new architecture for implementing unidirectional and bidirectional self-healing interoffice ring networks using wavelength division multiplexing (WDM) technology for growth is proposed. The working ring uses all-optical drop-and-add multiplexers at each office and the protection path uses optical amplifiers and optical switches. The use of WDM technology, combined with all-optical drop-and-add multiplexers, accommodates growth to a network capacity of more than 1 Tb/s.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Biological Mechanisms of Fluorosis and Level and Timing of Systemic Exposure to Fluoride with Respect to Fluorosis (1992)

Enamel fluorosis can occur following either an acute or chronic exposure to fluoride during tooth formation. Fluorosed enamel is characterized by a retention of amelogenins in the early-maturation stage, and by the formation of a more porous enamel with a subsurface hypomineralization. The mechanisms by which fluoride affects enamel development include specific effects on both the ameloblasts and on the developing enamel matrix. Maturation-stage ameloblast modulation is more rapid in fluorosed enamel as compared with control enamel, and proteolytic activity in fluorosed early-maturation enamel is reduced as compared with controls. Secretory enamel appears to be more susceptible to the effects of fluoride following acute fluoride exposure, such as may occur with the use of fluoride supplements. However, both human and animal studies show that the transition/early-maturation stage of enamel formation is most susceptible to the effects of chronic fluoride ingestion at above-optimal levels of fluoride in drinking water.
OVVV: Using Virtual Worlds to Design and Evaluate Surveillance Systems (2007)

Object video virtual video (OVVV) is a publicly available visual surveillance simulation test bed based on a commercial game engine. The tool simulates multiple synchronized video streams from a variety of camera configurations, including static, PTZ and omni-directional cameras, in a virtual environment populated with computer or player controlled humans and vehicles. To support performance evaluation, OVVV generates detailed automatic ground truth for each frame including target centroids, bounding boxes and pixel-wise foreground segmentation. We describe several realistic, controllable noise effects including pixel noise, video ghosting and radial distortion to improve the realism of synthetic video and provide additional dimensions for performance testing. Several indoor and outdoor virtual environments developed by the authors are described to illustrate the range of testing scenarios possible using OVVV. Finally, we provide a practical demonstration of using OVVV to develop and evaluate surveillance algorithms.
Extraordinarily high-contrast and wide-view liquid-crystal displays (2005)

A computer simulation model based on oblique-angle Jones matrix and Poincaré sphere is developed for optimizing the design of film-compensated multidomain vertical-alignment liquid crystal display (VA-LCD). According to this design, a contrast ratio higher than 10 000:1 is predicted over the entire ±85° viewing cone for the four-domain VA-LCD. Potential application for liquid-crystal display television is emphasized.
Type I-E CRISPR-Cas System as a Defense System in Saccharomyces cerevisiae (2022)

Defense against viruses and other mobile genetic elements (MGEs) is important in many organisms. The CRISPR-Cas systems found in bacteria and archaea constitute adaptive immune systems that can acquire the ability to target previously unrecognized MGEs. No CRISPR-Cas system is found to occur naturally in eukaryotic cells, but here, we demonstrate interference by a type I-E CRISPR-Cas system from Escherichia coli introduced in Saccharomyces cerevisiae. The designed CRISPR arrays are expressed and processed properly in S. cerevisiae. Targeted plasmids display reduced transformation efficiency, indicative of DNA cleavage. <b>IMPORTANCE</b> Genetic inactivation of viruses and other MGEs is an important tool with application in both research and therapy. Gene editing using, e.g., Cas9-based systems, can be used to inactivate MGEs in eukaryotes by introducing specific mutations. However, type I-E systems processively degrade the target which allows for inactivation without detailed knowledge of gene function. A reconstituted CRISPR-Cas system in S. cerevisiae can also function as a basic research platform for testing the role of various factors in the interference process.
Alopecia - the search for novel agents continues (1997)

Recently, the search for new and effective agents for the treatment of alopecia has become significantly more intense. The increase in hair biology research worldwide seen in both academic institutions and pharmaceutical companies stems from the desire to profit from the marketing of drugs that have been termed cosmeceuticals. Millions of men and women from every ethnic group suffer from various forms of alopecia, the most common being androgenetic alopecia (AGA), where the target tissue active androgen, 5α-dihydrotestosterone (DHT) aggravates genetically programmed scalp hair follicles, resulting in hair loss. Currently available drugs indicated for other disease processes are still commonly used to treat the various forms of alopecia because no other agents are available; some of these compounds have severe side-effects and many also exhibit minimal efficacy. These prescription drugs were not originally indicated for alopecia and have not been adequately tested in controlled clinical trials to assess for efficacy, safety and toxicity. Despite this, these agents continue to be used clinically for the treatment of patients with various forms of alopecia. To combat the problems associated with the currently prescribed drugs a variety of new agents have emerged in patent applications. This report reviews nearly 70 patent applications submitted since 1995 for AGA, immunomodulatory related hair diseases and antichemotherapeutic alopecia agents (preventing hair loss during chemotherapy) and discusses the mechanisms of action targetted by research and their implications regarding efficacy.