gebhart/patrepeval huggingface dataset:
Dissecting the centromere of the human Y chromosome with cloned telomeric DNA (1994)

We have used telomeric DNA to break the human Y chromosome within the centromeric array of alphoid satellite DNA and have created two derivative chromosomes; one consists of the short arm and 140 kb of alphoid DNA, the other consists of the long arm and 480 kb of alphoid DNA. Both segregate accurately at mitosis. It is known that there is no large scale sequence duplication around the alphoid DNA and so the simplest interpretation of our results is that the sequence responsible for accurate segregation is the alphoid DNA itself. Although the long arm acrocentric derivative segregates accurately it lags with respect to the other chromosomes in about 10% of anaphase cells and thus additional sequences may be required for orderly segregation. The short arm acrocentric chromosome is probably no larger than 12 Mb in size and thus our results also demonstrate that chromosomes of this size are capable of accurate segregation.
Preimplantation Genetic Diagnosis and Screening (2005)

Preimplantation genetic diagnosis (PGD) identifies genetic abnormalities in preimplantation embryos prior to embryo transfer. PGD is an exciting technology that may improve the likelihood of a successful pregnancy and birth for five distinct patient groups: (1) those with infertility related to recurrent miscarriages or unsuccessful in vitro fertilization (IVF) cycles, (2) those with unexplained infertility, (3) advanced maternal age, (4) severe male factor infertility, and (5) couples at risk for transmitting a hereditary disease to their offspring. PGD is always performed following an IVF cycle where multiple oocytes are retrieved and fertilized. Sophisticated techniques such as multiprobe, multicolor fluorescence in situ hybridization are used to test single cells for structural or numerical chromosome abnormalities, whereas the polymerase chain reaction, linkage analysis, and DNA sequencing are used to analyze single cells for disease-specific DNA mutations. PGD allows one to transfer only those embryos identified as being free of genetic abnormalities, thus potentially increasing the implantation rate and decreasing the miscarriage rate. These technologies identify embryos free of specific genetic abnormalities and may increase the likelihood of achieving the patient's goal: the birth of a healthy infant.
Rituximab as rescue therapy in anti-neutrophil cytoplasmic antibody-associated vasculitis: a single-centre experience with 15 patients (2008)

We report the largest case series of rituximab use for ANCA-associated vasculitis so far. Our data support that the drug is capable of inducing partial or complete remission in refractory or relapsing patients. Leucopenia and infectious complications remain a matter of concern.
Metalorganic Chemical Vapor Deposition of III-V Semiconductors (1984)

Metalorganic chemical vapor deposition (MOCVD) is a process in which two or more metalorganic chemicals (for instance, trimethylgallium) or one or more metalorganic sources and one or more hydride sources (for instance, arsine, AsH(3)) are used to form the corresponding intermetallic crystalline solid solution. MOCVD materials technology is a vapor-phase growth process that is becoming widely used to study the basic physics of novel materials and to grow complex semiconductor device structures for new optoelectronic and photonic systems. The MOCVD process is described and some of the device applications and results that have been realized with it are reviewed, with particular emphasis on the III-V compound semiconductors.
Nanofibres and their Influence on Cells for Tissue Regeneration (2005)

Synthetic polymer and biopolymer nanofibres can be fabricated through self-assembly, phase separation, electrospinning, and mechanical methods. These novel functional biocompatible polymers are very promising for a variety of future biomedical applications. There are many characteristics of nanofibres that would potentially influence cell growth and proliferation. As such, many studies have been carried out to elucidate the cell–nanofibre interaction with the purpose of optimizing the matrix for cell growth and tissue regeneration. In this Review, we present current literatures and our research on the interactions between cells and nanofibres, and the potentials of nanofibre scaffolds for biomedical applications.
Platelet-derived growth factor agonist activity of a secreted form of the v-sis oncogene product. (1985)

We have compared the functional properties of a growth factor partially purified from medium conditioned by simian sarcoma virus-transformed cells with those of platelet-derived growth factor (PDGF). The factor mimicked the effects induced by PDGF: it bound to and activated human fibroblast PDGF receptors and stimulated DNA synthesis. These activities were specifically inhibited by PDGF antibodies and thus elicited by a factor(s) immunologically related to PDGF. The factor behaved as a secretory protein, since about 95% of the receptor-binding activity was found in the medium after a 48-hr serum-free incubation. Structural characterization of the PDGF-like activity revealed a Mr 24,000 intracellular protein and two polypeptides of Mr 13,000 and 11,500 released into the medium. The Mr 13,000 component bound to human fibroblasts; this binding was competitively inhibited by PDGF. The data support the possibility that oncogene products may elicit transforming activity by interacting with the normal cellular mitogenic pathway.
Separation and Purification Techniques in Biotechnology (1990)

This book describes separation and purification techniques adsorption, ion exchange and liquid chromatography on solid supports used for fermentation and biochemical feedstreams. Emphasis is placed on basic sorption theory, laboratory evaluation techniques, sorptive materials and their characteristics, scale-up of laboratory techniques, and their industrial applications. Each chapter contains specific examples illustrating the use of purification techniques in biotechnology processes.
Antigen-specific activity of carcinoma-reactive BR64-doxorubicin conjugates evaluated in vitro and in human tumor xenograft models. (1992)

The anticarcinoma antibody BR64 was conjugated to a doxorubicin derivative, doxorubicin 13-[3-(2-pyridyldithio)propionyl]hydrazone, and the resulting conjugates (BR64-DOX) were evaluated for activity and immunological specificity in vitro and in human tumor xenograft models. The BR64-DOX immunoconjugates retained immunoreactivity and cytotoxicity and demonstrated antigen-specific cytotoxicity in vitro. The potency of BR64-DOX immunoconjugates in vitro was related to the drug:monoclonal antibody mole ratio of the conjugates. The antitumor activity of BR64-DOX conjugates was consistently superior to the maximal activity obtained with the parent drug, doxorubicin (DOX), in established human lung and human breast carcinoma xenograft models. The superior antitumor activity of BR64-DOX conjugates was reflected both in tumor growth inhibition and in regressions and cures of established tumors following the administration of tolerated doses of BR64-DOX. The antitumor activity of BR64-DOX conjugates was not the result of synergism between monoclonal antibody BR64 and DOX, because mixtures consisting of monoclonal antibody and optimized DOX were not more active than an equivalent dose of DOX administered alone. The antitumor activity of BR64-DOX conjugates was antigen specific; equivalent doses of nonbinding isotype-matched conjugates were not active against established tumor xenografts.
Morphological signal adaptive median filter for still image and image sequence filtering (2002)

A novel extension of the classical signal-adaptive median filter (SAM) is proposed in this paper, namely the morphological signal-adaptive median filter (MSAM). Three modifications are introduced in the SAM filter aiming at: (1) enhancing the SAM impulse detection mechanism so that it also detects randomly-valued impulses, (2) employing an anisotropic window adaptation based on binary morphological erosions/dilations with predefined structuring sets and (3) extending its design for its application to image sequences as well. Its performance has been tested for noise suppression in both still images and image sequences. Its robustness against a wide variety of noise distributions as well as its superiority to the classical SAM filter are proved judging from both objective (SNR, MAE) and subjective (perceived image quality) criteria.
Systemically Administered Interleukin-10 Reduces Tumor Necrosis Factor-Alpha Production and Significantly Improves Functional Recovery Following Traumatic Spinal Cord Injury in Rats (1999)

In these studies, we examined the neuroprotective effects of the potent antiinflammatory cytokine interleukin-10 (IL-10) following spinal cord injury (SCI). Neuroprotection was assessed by using behavioral and morphological end points. We hypothesized that injury-induced inflammation contributes to the resulting neuropathology and subsequent loss of function. Therefore, by attenuating injury-induced inflammation, we should promote functional recovery. The New York University device was used to induce moderate SCI and study the resulting inflammatory response and functional consequences of inhibiting this response in rats. We determined that SCI induces the expression of tumor necrosis factor-alpha (TNF-alpha) in the spinal cord and by SCI-activated monocytes isolated from the peripheral circulation. IL-10 (5.0 microg) administered 30 minutes after-injury significantly reduced the expression of TNF-alpha protein in the spinal cord and in vitro by SCI-activated monocytes. Next, we investigated whether IL-10 would improve functional recovery after SCI. Randomized, double-blinded studies demonstrated that a single injection of IL-10 significantly improves hind limb motor function 2 months after injury, as determined by the Basso, Beattie and Bresnahan (BBB) open-field behavioral test. IL-10-treated animals had a mean BBB score of 18.0+/-0.5 (SEM, n = 9) compared with a score of 12.9+/-0.6 (SEM, n = 9) for the saline-treated controls. Morphological analysis demonstrated that IL-10 reduces lesion volume by approximately 49% 2 months after injury. These data suggest that acute administration of IL-10 reduces TNF-alpha synthesis in the spinal cord and by activated macrophages, is neuroprotective, and promotes functional recovery following SCI.
Interferon-γ Induced Medulloblastoma in the Developing Cerebellum (2004)

We have generated a mouse model system with a high incidence of medulloblastoma, a malignant neoplasm believed to arise from immature precursors of cerebellar granule neurons. These animals ectopically express interferon-gamma (IFN-gamma) in astrocytes in the CNS in a controlled manner, exploiting the tetracycline-controllable system. More than 80% of these mice display severe ataxia and develop cerebellar tumors that express synaptophysin, the mouse atonal homolog MATH1, sonic hedgehog (SHH), and Gli1. IFN-gamma-induced tumorigenesis in these mice is associated with increased expression of SHH, and SHH induction and tumorigenesis are dependent on signal transducer and activator of transcription 1 (STAT1). When IFN-gamma expression is shut down with doxycycline at postnatal day 12 (P12), the clinical symptoms dissipate and the mice do not develop tumors, whereas if transgene expression is shut down at P16, the clinical symptoms and tumors progress to lethality, indicating that IFN-gamma is required for tumor induction but not progression. The tumors that occur in the continued presence of IFN-gamma display extensive necrosis and apoptosis as well as macrophage and lymphocytic infiltration, whereas the tumors that develop in mice in which IFN-gamma expression is shut down at P16 do not. Thus, IFN-gamma expression in the perinatal period can induce SHH expression and medulloblastoma in the cerebellum by a STAT1-dependent mechanism, and its continued presence appears to promote a host response to the tumor.
Role of CD27/CD70 pathway of activation in immunity and tolerance (2010)

The CD70/CD27 axis has gained increasing interest among the immunologists, because of its capacity to regulate immunity versus tolerance. Recent studies clearly show that expression of CD70 may prevent tolerance induced by antigen presentation in the steady-state, i.e., by nonactivated DCs. In addition, CD27 signaling appears critical for T cell expansion and survival and therefore, induction of long-term memory. It contributes to germinal center formation, B cell activation, and production of neutralizing antibodies but can also be subverted by viruses, in particular, during chronic infections. The potential role of the CD27/CD70 pathway in the course of inflammatory diseases, as in EAE, arthritis, and inflammatory bowel disease models, suggests that CD70 may be a target for immune intervention. Conversely, the potency of costimulation through CD27 suggests that the CD27/CD70 axis could be exploited for the design of anti-cancer vaccines.
A completely implantable total artificial heart system. (1992)

The authors developed, built, and tested in vivo a completely implanted total artificial heart (TAH) system. The system used a reduced size version of a roller screw energy converter and mating sac blood pumps. The motor drive, pumps, and a compliance chamber were implanted intrathoracically. A canister containing controlling electronics and an emergency battery was implanted in the abdomen. The secondary coil of an inductive energy transmission and telemetry system was placed over the ribs. The system was implanted in three calves, that survived 0.5-13 days with the system. The system maintained safe left atrial pressures and adequate cardiac outputs during each animal's entire course.
Synthesis of Methylene- and Alkylidenecyclopropane Derivatives (1998)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Methylene- and Alkylidenecyclopropane Derivatives†Alberto Brandi and Andrea GotiView Author Information Dipartimento di Chimica Organica "Ugo Schiff", Centro di Studio sulla Chimica e la Struttura dei Composti Eterociclici e loro Applicazioni (CSCEA)C.N.R., Università degli Studi di Firenze, via G. Capponi 9, I-50121 Firenze, Italy Cite this: Chem. Rev. 1998, 98, 2, 589–636Publication Date (Web):February 7, 1998Publication History Received7 May 1997Revised15 October 1997Published online7 February 1998Published inissue 1 April 1998https://pubs.acs.org/doi/10.1021/cr940341thttps://doi.org/10.1021/cr940341tresearch-articleACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views2883Altmetric-Citations287LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alcohols,Allenes,Hydrocarbons,Reaction products,Rearrangement Get e-Alerts
Impairment of reward-related learning by cholinergic cell ablation in the striatum (2003)

The striatum in the basal ganglia-thalamocortical circuitry is a key neural substrate that is implicated in motor balance and procedural learning. The projection neurons in the striatum are dynamically modulated by nigrostriatal dopaminergic input and intrastriatal cholinergic input. The role of intrastriatal acetylcholine (ACh) in learning behaviors, however, remains to be fully clarified. In this investigation, we examine the involvement of intrastriatal ACh in different categories of learning by selectively ablating the striatal cholinergic neurons with use of immunotoxin-mediated cell targeting. We show that selective ablation of cholinergic neurons in the striatum impairs procedural learning in the tone-cued T-maze memory task. Spatial delayed alternation in the T-maze learning test is also impaired by cholinergic cell elimination. In contrast, the deficit in striatal ACh transmission has no effect on motor learning in the rota-rod test or spatial learning in the Morris water-maze test or on contextual- and tone-cued conditioning fear responses. We also report that cholinergic cell elimination adaptively up-regulates nicotinic ACh receptors not only within the striatum but also in the cerebral cortex and substantia nigra. The present investigation indicates that cholinergic modulation in the local striatal circuit plays a pivotal role in regulation of neural circuitry involving reward-related procedural learning and working memory.
Expression of p53 and CD44 in Canine Breast Tumor (2008)

The p53 and CD44 expression of 10 cases in canine breast tumor were examined utilizing immunohistochemical assay with rabbit anti-mouse polyclonal antibodies against p53 or CD44, respectively. The p53 expression was significantly higher in malignant than in benign breast tumor. The expression of CD44 was not significantly different in malignant breast cancer and benign breast tumor. This suggests that p53 can be used as an indicator for animal prognosis.
공업화자재의 활용을 통한 공정합리화 (2003)

도심지 건축공사가 증가하고 있는 우리나라에서 작업공간의 부족 및 자재의 적시공급에 대한 문제는 반드시 해결해야 할 과제이다. 이 중에서도 주택건설에 있어 가장 큰 비중을 차지하고 있는 아파트 시공의 경우, 초고층화로 골조공사의 중요성과 함께 친환경적 공법과 합리적 공정개선 및 원가절감을 구현할 새로운 공법이 요구되고 있다. 본 연구에서는 국내 건설현장의 사례조사를 통해 슬래브 거푸집공사와 마감공사의 프로세스에서 불필요한 요소를 최소화하고자 공업화 자재를 활용한 (골조-마감공사)통합공법을 제시한다. 가치흐름분석기법(Value Stream Mapping)을 활용하여 기존 프로세스에서의 비가치창출작업을 가치창출작업에 통합시키거나 제거하여 슬래브 공사 내에서 공기 및 투입인력의 절감 가능성을 확인하고, 공기단축과 원가절감 효과를 측정하였다. 이와 같이 공업화 자재의 활용으로 현장작업에 따른 변이를 최소화하여 공정의 안정성 및 공정계획의 신뢰성을 확보 등 공정의 합리화를 구현하고자 한다.
Modulation of cytokine expression by CD4+ T cells during coxsackievirus B3 infections of BALB/c mice initiated by cells expressing the gamma delta + T-cell receptor (1996)

Two variants of coxsackievirus B3 have been used to investigate the pathogenesis of myocarditis in BALB/c mice. H3 virus induces moderate myocarditis and H310A1 virus induces minimal myocarditis, although both viruses infect and replicate in the heart. Cells expressing the gamma delta+ T-cell receptor composed 5 to 13% of the lymphocytes infiltrating the hearts of H3 virus-infected mice and belonged to either the CD4- CD8+ gamma delta+- or CD4- CD8- gamma delta+-cell population. Giving 5,000 gamma delta+ cells isolated from the hearts of H3 virus-infected mice to H310A1 virus-infected recipients restored myocarditis susceptibility in the recipient animals and shifted the pattern of cytokine production in the virus-immune CD4+-cell population from being predominantly interleukin-4 producing to being predominantly gamma interferon producing in the H310A1 virus-infected mice. Apoptosis was evident in the infiltrating lymphocyte population in the myocardia of H3 virus-infected mice by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling assay and in splenic lymphocytes by DNA fragmentation in agarose gel electrophoresis and was confined to the CD4+ population. No apoptosis was observed in H310A1 virus-infected mice, but apoptosis was induced subsequent to gamma delta +-T-cell transfer. These results are consistent with the hypothesis that gamma delta+ T cells may help modulate cytokine responses during virus infections in vivo and that apoptosis might be involved in this modulation.
Epithelial Dysplasia in Nipple Aspirates of Breast Fluid: Association With Family History and Other Breast Cancer Risk Factors<xref ref-type="fn" rid="FN2">2</xref><xref ref-type="fn" rid="FN3">3</xref> (1982)

Because of the strong association of epithelial dysplasia and breast cancer, the extent to which dysplasia of breast fluid epithelial cells obtained by nipple aspiration is associated with breast cancer risk factors was investigated. A significantly higher proportion of women who had a first-degree family history of breast cancer had dysplastic cells in breast fluid than women who had no such history. When family history status was correlated with various breast cancer risk factors, higher proportions of women with dysplasia were found among those who had a first degree family history of breast cancer and for whom certain other risk factors were present, which suggested additive and synergistic effects of these factors on breast epithelium. Breast epithelia of women with a first-degree family history of breast cancer may be more prone to abnormal differentiation in response to environmentally conditioned risk factors.
N-(L-a-Aminoacyl) Derivatives of Methotrexate (1989)

nethotrexate di-t-butvl ester 3 was coupled with Nt-butvloxvcarbonvl-L-leucine bv the o -n i t r o ~h e n v l ester and carbodiimide methods t o give the di-t-butvl esters & -7a of 2-.4-. and 2.4-dilN-t-butvloxvcarbonvl-L-leuc~1~methot~exate.T h e CorresDonding L-alanvl analogues 3were also svnthesised bv the latter method.The positions of the acvl qroups were determined from "C-nmr and uv data.Upon deprotection with trifluoroacetic acid, the 2-acvl ~r o d u c t s a and 5b vielded 2-L-leucvl-and 2-L-alanvlmethotreuate Q and Ob, but the 4-acvl analogues 6 -and & gave decomDosition -
Design, synthesis, and conformational studies of the hGM‐CSF derived peptide (13–27)–Gly–(75–87) (1999)

An analogue of the human granulocyte–macrophage colony-stimulating factor (hGM-CSF), hGM-CSF(13–27)–Gly–(75–87) was synthesized by solid phase methodology. This analogue was designed to comprise helices A and C of the native growth factor, linked by a glycine bridge. Helices A and C form half of a four-helix bundle motif in the crystal structure of the native factor and are involved in the interaction with α- and β-chains of the heterodimeric receptor. A conformational analysis of the synthetic analogue by CD, two-dimensional nmr spectroscopy, and molecular dynamics calculations is reported. The analogue is in a random structure in water and assumes a partially α-helical conformation in a 1 : 1 trifluoroethanol/water mixture. The helix content in this medium is ∼ 70%. By 2D-nmr spectroscopy, two helical segments were identified in the sequences corresponding to helices A and C. In addition to medium- and short-range NOESY connectivities, a long-range cross peak was found between the Cβ proton of Val16 and NH proton of His87 (using the numbering of the native protein). Experimentally derived interproton distances were used as restraints in molecular dynamics calculations, utilizing the x-ray coordinates as the initial structure. The final structure is characterized by two helical segments in close spatial proximity, connected by a loop region. This structure is similar to that of the corresponding domain in the x-ray structure of the native growth factor in which helices A and C are oriented in an antiparallel fashion. The N-terminal residues Gly–Pro of helix C are involved in an irregular turn connecting the two helical segments. As a consequence, helix C is appreciably shifted and slightly rotated with respect to helix A compared to the x-ray structure of the native growth factor. These small differences in the topology of the two helices could explain the lower biological activity of this analogue with respect to that of the native growth factor. © 1999 John Wiley & Sons, Inc. Biopoly 50: 545–554, 1999