gebhart/patrepeval huggingface dataset:
Comparative Trial of Tenormin (Atenolol) and Inderal (Propranolol) in Migraine (1980)

Atenolol, 50 mg, b.i.d., and propranolol, 80 mg b.i.d.,. were given to 28 patients with a presumed diagnosis of migraine, in a placebo-controlled double-blind cross-over study, where each period lasted 6 weeks. In the total material, atenolol was significantly better than placebo in reducing attacks, whereas propranolol showed no definite such effect. In seven patients the number of headache days was remarkably high (average: 22 headache days in 42 days), which may indicate that they have been inappropriately included in the material or have recorded interparoxysmal headache in addition to attacks. If these patients are excluded, the attack-reducing effect of propranolol and tenormin is significant and of equal magnitude.
Obstacle avoidance and concealed target detection using the Army Research Lab ultra-wideband synchronous impulse reconstruction (UWB SIRE) forward imaging radar (2007)

The U.S. Army Research Laboratory (ARL), as part of a mission and customer funded exploratory program, has developed a new low-frequency, ultra-wideband (UWB) synthetic aperture radar (SAR) for forward imaging to support the Army's vision of an autonomous navigation system for robotic ground vehicles. These unmanned vehicles, equipped with an array of imaging sensors, will be tasked to help detect man-made obstacles such as concealed targets, enemy minefields, and booby traps, as well as other natural obstacles such as ditches, and bodies of water. The ability of UWB radar technology to help detect concealed objects has been documented in the past and could provide an important obstacle avoidance capability for autonomous navigation systems, which would improve the speed and maneuverability of these vehicles and consequently increase the survivability of the U. S. forces on the battlefield. One of the primary features of the radar is the ability to collect and process data at combat pace in an affordable, compact, and lightweight package. To achieve this, the radar is based on the synchronous impulse reconstruction (SIRE) technique where several relatively slow and inexpensive analog-to-digital (A/D) converters are used to sample the wide bandwidth of the radar signals. We conducted an experiment this winter at Aberdeen Proving Ground (APG) to support the phenomenological studies of the backscatter from positive and negative obstacles for autonomous robotic vehicle navigation, as well as the detection of concealed targets of interest to the Army. In this paper, we briefly describe the UWB SIRE radar and the test setup in the experiment. We will also describe the signal processing and the forward imaging techniques used in the experiment. Finally, we will present imagery of man-made obstacles such as barriers, concertina wires, and mines.
Efficient mono- and bis-functionalization of 3,6-dichloropyridazine using (tmp)2Zn·2MgCl2·2LiCl (2008)

3,6-Dichloropyridazine undergoes a smooth metallation using (tmp)2Zn.2MgCl2.2LiCl. The resulting bis-organozinc species react with various electrophiles; subsequent functionalization via a second metallation proceeds readily; further reactions with hydrazine lead to highly substituted pyrazolo[3,4-c]pyridazines derivatives.
Genetically Engineered Polymers for Drug Delivery (1999)

Genetic engineering methodology offers the ability to synthesize protein-based polymers with precisely controlled structures. Protein-based polymers synthesized by recombinant techniques have a well-defined monomer composition and sequence, stereochemistry, and a narrow molecular weight distribution. The structure of the polymeric carrier at the molecular level influences its biological disposition and drug release profile. Current methodologies of polymer synthesis (chemical polymerization) result in the production of polymers with heterogeneous molecular weights, and with monomer sequences and compositions defined in terms of statistical distributions. Genetic engineering methodologies can be used to design new polymeric drug carriers with improved properties, such as better-defined biorecognition, pharmacokinetic, biodegradation, and drug release profiles. In this review article the rationale and methodology of polymer synthesis using genetic engineering techniques, the status of such polymers in drug delivery to-date, and the potential of these polymers for the development of new systems in the future are discussed.
Drilling a horizontal coalbed methane drainage system from a directional surface borehole (1982)

Three long horizontal holes were drilled from a directionally drilled surface hole at the Emerald Mine near Waynesburg, Penn. The purpose was to adapt the technique of directional drilling for use in draining methane from coal beds. A 504-m long, 76-mm diameter, circular arc pilot hole was drilled, using a down-hole mud motor, to enter the Pittsburgh coal bed at a vertical depth of 305 m. The hole was reamed to 222 mm and was cased to a measured depth of 486 m. Three 76-mm diameter horizontal drainage holes were drilled, totaling 2909 m of horizontal hole. Improvements in drilling methods increased the average drilling rate from 20.4 to 64.9 m/day. The cost of drilling the directional and horizontal holes was $1,169,530, a figure inflated by inexperience and by delays caused by lost-circulation problems and fishing operations. An estimated total drilling cost of $960,000 for an improved system was determined by a detailed cost analysis. Initial gas and water production from Nov. 1979 through May 1980 was low because of caving of the horizontal holes drilled in shale near the bottom of the casing.
Neural Crest Cells Retain Multipotential Characteristics in the Developing Valves and Label the Cardiac Conduction System (2006)

Multipotent neural crest cells (NCCs) are a major extracardiac component of cardiovascular development. Although recognized as contributing cells to the arterial valves at early developmental stages, NCC persistence in the valves at later times or in the adult heart is controversial. We analyzed NCC persistence and contributions to both semilunar and atrioventricular (AV) valves in the mature heart. Two NCC-specific promoters driving Cre recombinase, Wnt1-Cre and P0-Cre, were mated with floxed reporter mice, R26R or CAG-CAT-EGFP, to map NCC fate. Hearts were analyzed before aorticopulmonary (AP) septation through adult stages. As previously demonstrated, strong NCC labeling was detected in ventral and dorsal outflow cushions before AP septation. In contrast to previous reports, we found that substantial numbers of labeled cells persisted in the semilunar valves in late fetal, neonatal, and adult hearts. Furthermore, NCCs were also found in the AV valves, almost exclusively in the septal leaflets. NCCs in the AV valves expressed melanocytic and neurogenic markers. However, cells labeled in the proximal cardiac conduction system exhibited neurogenic and gliagenic markers, whereas some NCCs expressed no differentiation specific markers. These results suggest that cardiac NCCs contribute to the mature valves and the cardiac conduction system and retain multipotent characteristics late in development.
Studies on the structure and intrinsic innervation of the normal human prostate (1989)

Prostates from eight adult males (age range 17-63 years) were employed in this investigation. Six glands were obtained from multiorgan transplantation donors at the time of organ harvesting, and two additional glands, at routine post-mortem. Microscopic examination has shown that the majority of acini in the peripheral parts of the gland were individually relatively small and lined by an epithelium two to three cells in thickness. In contrast, acini in the central part of the gland were usually large and contained numerous prominent epithelial folds within the lumen. On the basis of morphology, a clear distinction between peripheral and central acini was not observed. The distribution of autonomic ganglia both within and adjacent to the prostate was studied, and nerve cell bodies containing both acetylcholinesterase (AChE) activity and neuropeptide immunoreactivity were identified. The distribution and density of AChE-positive nerves associated with smooth muscle in either the peripheral or central parts of the prostate were indistinguishable. In addition, the majority of acini in peripheral and central regions possessed a rich subepithelial plexus of autonomic nerves. VIP-positive nerve fibers were found in relation to the epithelium lining acini in central and peripheral regions of the gland. In contrast, nerves possessing NPY immunoreactivity occurred only in relation to the smooth muscle trabeculae of the prostate.
Bacopa monniera Linn. as an antioxidant: mechanism of action. (1996)

Bacopa monniera, Linn. (Brahmi: Scrophulariaceae) an Ayurvedic medicine is clinically used for memory enhancing, epilepsy, insomnia and as mild sedative. For the first time the effect of alcohol and hexane fraction of Brahmi has been studied on FeSO4 and cumene hydroperoxide induced lipid peroxidation. Alcohol fraction showed greater protection with both inducers. Results were compared with known antioxidants tris, EDTA and a natural-antioxidant vitamin E. The effect of Brahmi was also examined on hepatic glutathione content. The mechanism of action could be through metal chelation at the initiation level and also as chain breaker. The results suggested that Brahmi is a potent antioxidant. The response of Brahmi was dose dependent. Tris, an hydroxyl trapper did not show any protection in comparison to Brahmi where as EDTA and vitamin E did protect against FeSO4. In experimental conditions 100 micrograms Brahmi extract (alcoholic) was equivalent to 247 micrograms of EDTA (0.66 microM) and 58 micrograms of vitamin E. Interestingly Brahmi only slightly protected the autooxidation and FeSO4 induced oxidation of reduced glutathione on lower doses 100 micrograms/ml and below, but on higher concentrations it enhanced the rate of oxidation.
Purification of dermonecrotic toxin from a sonic extract of Pasteurella multocida SP-72 serotype D (1984)

A procedure was developed to purify dermonecrotic toxin (DNT) from a sonic extract of a serotype D strain of Pasteurella multocida. Sonic extract containing DNT was applied to a DEAE-Sephacel column and eluted by a linear gradient of NaCl. Upon rechromatographing, fractions with dermonecrotic activity for guinea pigs were applied on a second Sephacel column, and a pooled fraction with the toxic activity was filtered through a Sephadex G-200 column. Pooled fractions with the toxic activity were subjected to polyacrylamide disc gel electrophoresis (PAGE), and the toxic substance was eluted from each sliced gel. Eluted fractions with the toxic activity were rechromatographed on a second Sephadex G-200 column, and a pooled fraction with high dermonecrotic activity was referred to as a purified DNT. The activity of purified DNT was increased by 1,000 times, and the average yield was about 1.8%. The purified DNT was homogeneous as determined by Ouchterlony double immunodiffusion, crossed immunoelectrophoresis, and thin-layer isoelectric focusing in polyacrylamide gels and gave a single band on PAGE and sodium dodecyl sulfate-PAGE. The molecular weight of the toxin was ca. 160,000 as determined by sodium dodecyl sulfate-PAGE. The isoelectric point of the toxin was ca. 4.7 to 4.8. Amino acid analysis of the purified DNT revealed that the toxin was composed of characteristically high proportions of glutamic acid, aspartic acid, glycine, proline, alanine, and leucine. The minimal necrotizing dose of the toxin was about 1 ng of protein, and the 50% lethal dose per mouse was 0.2 micrograms. The purified DNT was heat labile and sensitive to inactivation by trypsin, Formalin, and glutaraldehyde.
Expression of 72 kilodalton type IV collagenase (gelatinase A) in benign and malignant ovarian tumors. (1993)

The findings indicate that genes for the 72 kd type IV collagenase and for its substrate are simultaneously active in the same cells of the tumor stroma. The difference in the in situ hybridization and immunohistochemical findings could be explained by a possible variation in the metabolic balance between synthesis and accumulation of the protein in different cell types. It can also be proposed that the activity of the 72 kd type IV collagenase would be mediated through a receptor-like mechanism present on epithelial cells which could bind the 72 kd type IV collagenase synthesized elsewhere. There is also a possibility that the gelatinolytic activity of the mesenchymally synthesized 72 kd type IV collagenase would be consumed to degrade extracellular matrix proteins other than basement membranes.
Therapeutic Implications for the Induced Levels of Chk1 in Myc-Expressing Cancer Cells (2011)

Our data suggest that Chk1 inhibitors should be further evaluated as potential drugs against Myc-driven malignancies such as certain B-cell lymphoma/leukemia, neuroblastoma, and some breast and lung cancers.
Prediction of the structure of human Janus kinase 2 (JAK2) comprising the two carboxy-terminal domains reveals a mechanism for autoregulation (2001)

The structure of human Janus kinase 2 (JAK2) comprising the two C-terminal domains (JH1 and JH2) was predicted by application of homology modelling techniques. JH1 and JH2 represent the tyrosine kinase and tyrosine kinase-like domains, respectively, and are crucial for function and regulation of the protein. A comparison between the structures of the two domains is made and structural differences are highlighted. Prediction of the relative orientation of JH1 and JH2 was aided by a newly developed method for the detection of correlated amino acid mutations. Analysis of the interactions between the two domains led to a model for the regulatory effect of JH2 on JH1. The predictions are consistent with available experimental data on JAK2 or related proteins and provide an explanation for inhibition of JH1 tyrosine kinase activity by the adjacent JH2 domain.
Increased Mortality Associated with Growth Hormone Treatment in Critically Ill Adults (1999)

In patients with prolonged critical illness, high doses of growth hormone are associated with increased morbidity and mortality.
Role of Interleukin 8 in the Pathogenesis of Proliferative Vitreoretinopathy (1997)

We investigated the role of interleukin 8 (IL-8) in the pathogenesis of proliferative vitreoretinopathy (PVR). The IL-8 level in the vitreous and the blood of 20 patients with PVR was measured by a specific enzyme-linked immunoassay method. Vitreous from cadaver eyes (n = 20) was used as the control. The IL-8 level in the vitreous was between 31.3 and 65 pg/ml in 40% of eyes with PVR. IL-8 was detected neither in the blood of the patients with PVR nor in the vitreous from cadaver eyes. IL-8 levels in the vitreous did not correlate with either the grade of PVR or the duration of symptoms. These results suggest that IL-8 may play a role in the pathogenesis of PVR.
Significance of Fecal Coliform-Positive Klebsiella (1977)

A total of 191 Klebsiella pneumoniae isolates of human clinical, bovine mastitis, and a wide variety of environmental sources were tested for fecal coliform (FC) response with the membrane filtration and most probable number techniques. Twenty-seven Escherichia coli cultures of human clinical and environmental origins were also tested. Eighty-five percent (49/58) of known pathogenic K. pneumoniae were FC positive, compared with 16% (19/120) of the environmental strains. E. coli results indicated 93% (13/14) of the clinical and 85% (11/13) of the environmental strains as FC positive. There was no significant difference in the incidence of FC-positive cultures between pathogenic Klebsiella and E. coli. pH measurements of K. pneumoniae and E. coli cultures growing in m -FC broth at 44.5°C revealed three distinct pH ranges correlating with colony morphology. β-Galactosidase assays of Klebsiella and E. coli cultures at 44.5°C indicated all were able to hydrolyze lactose, even if they were FC negative by the membrane filtration or most probable number techniques. The FC response pattern appears stable in K. pneumoniae. Three pathogenic cultures showed no change in FC responses after 270 generations of growth in sterile pulp mill effluent. Since K. pneumoniae is carried in the gastrointestinal tract of humans and animals and 85% of the tested pathogenic strains were FC positive, the isolation of FC-positive Klebsiella organisms from the environment would indicate their fecal or clinical origin or both. The added fact that K. pneumoniae is an opportunistic pathogen of increasing importance makes the occurrence of FC-positive environmental Klebsiella , particularly in large numbers, a potential human and animal health hazard.
Growth of Fe doped semi-insulating GaN by metalorganic chemical vapor deposition (2002)

Iron doped GaN layers were grown by metalorganic chemical vapor deposition (MOCVD) using ferrocene as the Fe precursor. Specular films with concentrations up to 1.7×1019 cm−3, as determined by secondary ion mass spectrometry, were grown. The Fe concentration in the film showed a linear dependence on the precursor partial pressure, and was insensitive to growth temperature, pressure, and ammonia partial pressure. Memory effects were observed, similar to Mg doping of GaN by MOCVD. The deep acceptor nature of Fe was used for growth of semi-insulating GaN films on sapphire substrates. A 2.6-μm-thick GaN film with a resistivity of 7×109 Ω/sq was attained when the first 0.3 μm of the film was Fe doped. X-ray diffraction rocking curves indicated high crystalline quality, very similar to an undoped film, showing that Fe doping did not affect the structural properties of the film. Fe doping allows for growth of semi-insulating GaN on sapphire without the high threading dislocation densities and/or high carbon levels that are normally necessary to achieve insulating films.
Design of an artificial four-helix bundle metalloprotein via a novel ruthenium(II)-assisted self-assembly process (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDesign of an artificial four-helix bundle metalloprotein via a novel ruthenium(II)-assisted self-assembly processM. Reza Ghadiri, Christopher Soares, and Chong ChoiCite this: J. Am. Chem. Soc. 1992, 114, 10, 4000–4002Publication Date (Print):May 1, 1992Publication History Published online1 May 2002Published inissue 1 May 1992https://pubs.acs.org/doi/10.1021/ja00036a072https://doi.org/10.1021/ja00036a072research-articleACS PublicationsRequest reuse permissionsArticle Views654Altmetric-Citations139LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Binding patterns of co-existing aluminium and iron to human serum transferrin studied by HPLC–high resolution ICP-MS (2000)

Serum transferrin (Tf) is an iron-binding glycoprotein. Aluminium in the blood is bound to the transferrin. In the present study, the chemical forms of co-existing Al and Fe bound to human serum Tf were studied by combined on-line HPLC and high-resolution ICP-MS (HPLC-HR-ICP-MS). Samples were subjected to HPLC equipped with an anion-exchange column. The levels of 27Al, 56Fe and 32S, which are interfered with by polyatomic ions such as 13C14N+, 12C15N+ and 12C14N1H+, 40Ar16O+ and 40Ca16O+, and 16O2+, respectively, when using quadrupole ICP-MS, were monitored simultaneously by HR-ICP-MS at a resolution of m/delta m = 3000. Al added to apo-Tf as Al-citrate was preferentially bound to the N-lobe site almost selectively. Al in serum from a healthy person without any in vitro Al spike was present both as AlN-Tf and AlN,FeC-Tf. The chemical states were reproduced in the apo-Tf solution supplemented with Fe (Fe/Tf ratio = 0.6) and Al (Al/Tf ratio = 1) successively. The 32S level was useful for monitoring the protein levels in the HPLC eluate. The clean-up column procedures employed reduced the detection limit for 27Al to 0.1 microgram l-1 (3SB) at the middle resolution.
Dual effects of cytokines in regulation of MHC-unrestricted cell mediated cytotoxicity. (1993)

Natural killer (NK) cells probably function as an early defense line against viruses, due to their ability to kill virus-infected cells without resorting to clonal proliferation of memory cells. NK cells are also capable of killing tumor cells. In both cases the killing is major histocompatibility complex (MHC) unrestricted. NK cells exhibit spontaneous activity but they are positively regulated by interferon (IFN) or indirectly by such IFN-inducers as viruses, bacterial products, poly (rl):(rC), and mitogens. Interleukin-2 (IL-2) has the ability to enhance NK activity in addition to its ability to generate lymphokine-activated killer (LAK) cell activity. Recently, it was documented that other cytokines (e.g., IL-1, tumor necrosis factor (TNF), IL-6, and IL-12) are also involved in induction or enhancement of the cytotoxic activity of NK cells. Besides their "positive" regulation of NK activity, cytokines (in some cases the same cytokines) often act as "negative signals" for NK-mediated cytotoxicity. If NK susceptible target cells are exposed to IFN prior to NK cells, their sensitivity to NK activity is often markedly diminished. The mechanism by which IFNs (IFN-alpha, -beta and -gamma) affect the sensitivity of target cells to NK activity remains unknown, but it is clear that this function is not shared by other cell-mediated killing processes. The protective effect induced by IFN against NK activity is dependent on new mRNA and protein synthesis and can be abolished when target cells are incubated with combination of IFN and metabolic inhibitors or by chemotherapeutic purine or pyrimidine analogs. Class I MHC antigens have a central role in cell to cell interactions in the immune system. Because IFN has the ability to induce/increase class I MHC antigen expression, it has been suggested that class I MHC antigens act as "negative signals" of NK-mediated cytotoxicity. Although many studies support this hypothesis, others present evidence for a lack of involvement of class I MHC antigens in mediating sensitivity to NK activity. Other cytokines have been tested for their ability to affect the sensitivity of target cells to NK activity, as well as their ability to enhance the cytotoxic activity of NK effector cells. Lymphotoxin (TNF-beta) increases target cell susceptibility to NK activity. On the other hand, IL-1, IL-2, IL-6, and TNF reduce the sensitivity of target cells to NK lysis, at least in some systems.(ABSTRACT TRUNCATED AT 400 WORDS)
The role of the gallium scan in primary extranodal lymphoma. (1998)

The gallium scan was rarely positive for patients with skin, intestinal, testicular and low-grade lymphomas, but was otherwise comparable to lymphoma arising in lymph nodes. The result affected staging or treatment in seven patients (8%). After treatment, an initially-positive gallium scan usually became negative, a conversion associated with a favorable outcome.