gebhart/patrepeval huggingface dataset:
Effect of keratinocyte growth factor on cell invasion and migration in epithelial ovarian cancer cell line (2013)

Objective To examine the effect of keratinocyte growth factor(KGF) on cell invasion and migration in epithelial ovarian cancer cell lines,analyze the molecular mechanisms of KGF in the process of cell invasion and migration. Methods Human epithelial ovarian cancer cell line HO-8910PM and mice NIH3T3 fibroblast cell line were used. Cell wound scratch assay was used to examine the effect of different concentrations of KGF on the migration of the ovarian cancer cell line. MTT method was used to detect the proliferation ability of the ovarian cancer cell line. Western Blot and immumofluorescence method were used to detect the expression of KGF in the activated NIH3T3. Activation and induction effects of IL-1β,TGF-β1 and CM(conditioned medium of ovarian cancer cells) in the expression of KGF were observed. Results Cell wound scratch assay results showed that migration ability of the ovarian cancer cells was promoted by KGF,compared with the control group,KGF100 pmol/L group,KGF30 pmol/L group and KGF300 pmol/L group had statistically significant(P 0.01). MTT results showed that proliferation of the ovarian cancer cells were promoted by KGF,compared with the control group,KGF100 pmol/L group had statistically significant(P 0.01). Western Blot results showed the expression of KGF in fibroblast cell line was induced by IL-1β,TGF-β1 and CM. While immumofluorescence method results showed that IL-1β,TGF-β1 and CM could activate the fibroblast cell. Conclusion KGF can promote the proliferation and migration of the ovarian cancer cells,and IL-1β,TGF-β1,CM can promote the activation of the fibroblast,thereby to induce the expression of KGF on fibroblast.
Evolution-guided optimization of biosynthetic pathways (2014)

Engineering biosynthetic pathways for chemical production requires extensive optimization of the host cellular metabolic machinery. Because it is challenging to specify a priori an optimal design, metabolic engineers often need to construct and evaluate a large number of variants of the pathway. We report a general strategy that combines targeted genome-wide mutagenesis to generate pathway variants with evolution to enrich for rare high producers. We convert the intracellular presence of the target chemical into a fitness advantage for the cell by using a sensor domain responsive to the chemical to control a reporter gene necessary for survival under selective conditions. Because artificial selection tends to amplify unproductive cheaters, we devised a negative selection scheme to eliminate cheaters while preserving library diversity. This scheme allows us to perform multiple rounds of evolution (addressing ∼10(9) cells per round) with minimal carryover of cheaters after each round. Based on candidate genes identified by flux balance analysis, we used targeted genome-wide mutagenesis to vary the expression of pathway genes involved in the production of naringenin and glucaric acid. Through up to four rounds of evolution, we increased production of naringenin and glucaric acid by 36- and 22-fold, respectively. Naringenin production (61 mg/L) from glucose was more than double the previous highest titer reported. Whole-genome sequencing of evolved strains revealed additional untargeted mutations that likely benefit production, suggesting new routes for optimization.
[Analysis of circulating DNA level in the plasma of cervical cancer patients]. (2008)

Plasma DNA level is closely related with malignant transformation and development of cervical cancer, and may become a useful means for differential diagnosis of cervical cancer.
TRIPWIRE: A SYNCHRONISATION PRIMITIVE FOR VIRTUAL MEMORY MAPPED COMMUNICATION (2001)

Existing user-level network interfaces deliver high bandwidth, low latency performance to applications, but are typically unable to support diverse styles of communication and are unsuitable for use in multiprogrammed environments. Often this is because the network abstraction is presented at too high a level, and support for synchronisation is inflexible. In this paper we present a new primitive for in-band synchronisation: the Tripwire. Tripwires provide a flexible, efficient and scalable means for synchronisation that is orthogonal to data transfer. We describe the implementation of a non-coherent distributed shared memory network interface, with Tripwires for synchronisation. This interface provides a low-level communications model with gigabit class bandwidth and very low overhead and latency. We show how it supports a variety of communication styles, including remote procedure call, message passing and streaming.
Red cell membrane and plasma linoleic acid nitration products: Synthesis, clinical identification, and quantitation (2004)

Nitric oxide (*NO) and its reactive metabolites mediate the oxidation, nitration, and nitrosation of DNA bases, amino acids, and lipids. Here, we report the structural characterization and quantitation of two allylic nitro derivatives of linoleic acid (LNO(2)), present as both free and esterified species in human red cell membranes and plasma lipids. The LNO(2) isomers 10-nitro-9-cis, 12-cis-octadecadienoic acid and 12-nitro-9-cis, 12-cis-octadecadienoic acid were synthesized and compared with red cell and plasma LNO(2) species based on chromatographic elution and mass spectral properties. Collision-induced dissociation fragmentation patterns from synthetic LNO(2) isomers were identical to those of the two most prevalent LNO(2) positional isomers found in red cells and plasma. By using [(13)C]LNO(2) as an internal standard, red cell free and esterified LNO(2) content was 50 +/- 17 and 249 +/- 104 nM, respectively. The free and esterified LNO(2) content of plasma was 79 +/- 35 and 550 +/- 275 nM, respectively. Nitrated fatty acids, thus, represent the single largest pool of bioactive oxides of nitrogen in the vasculature, with a net LNO(2) concentration of 477 +/- 128 nM, excluding buffy coat cells. These observations affirm that basal oxidative and nitrating conditions occur in healthy humans to an extent that is sufficient to induce abundant membrane and lipoprotein-fatty acid nitration. Given that LNO(2) is capable of mediating cGMP and non-cGMP-dependent signaling reactions, fatty acid nitration products are species representing the convergence of ()NO and oxygenated lipid cell-signaling pathways.
Circulating Fibrocytes Prepare the Lung for Cancer Metastasis by Recruiting Ly-6C+ Monocytes Via CCL2 (2013)

Fibrocytes are circulating, hematopoietic cells that express CD45 and Col1a1. They contribute to wound healing and several fibrosing disorders by mechanisms that are poorly understood. In this report, we demonstrate that fibrocytes predispose the lung to B16-F10 metastasis by recruiting Ly-6C(+) monocytes. To do so, we isolated fibrocytes expressing CD45, CD11b, CD13, and Col1a1 from the lungs of wild type (WT) and Ccr5(-/-) mice. WT but not Ccr5(-/-) fibrocytes increased the number of metastatic foci when injected into Ccr5(-/-) mice (73 ± 2 versus 32 ± 5; p < 0.001). This process was MMP9 dependent. Injection of WT enhanced GFP(+) fibrocytes also increased the number of Gr-1(Int), CD11b(+), and enhanced GFP(-) monocytes. Like premetastatic-niche monocytes, these recruited cells expressed Ly-6C, CD117, and CD45. The transfer of these cells into Ccr5(-/-) mice enhanced metastasis (90 ± 8 foci) compared with B cells (27 ± 2), immature dendritic cells (31 ± 6), or alveolar macrophages (28 ± 3; p < 0.05). WT and Ccl2(-/-) fibrocytes also stimulated Ccl2 expression in the lung by 2.07 ± 0.05- and 2.78 ± 0.36-fold compared with Ccr5(-/-) fibrocytes (1.0 ± 0.06; p < 0.05). Furthermore, WT fibrocytes did not increase Ly-6C(+) monocytes in Ccr2(-/-) mice and did not promote metastasis in either Ccr2(-/-) or Ccl2(-/-) mice. These data support our hypothesis that fibrocytes contribute to premetastatic conditioning by recruiting Ly-6C(+) monocytes in a chemokine-dependent process. This work links metastatic risk to conditions that mobilize fibrocytes, such as inflammation and wound repair.
Gallstone Dissolution after 6 Months of Ursodeoxycholic Acid (UDCA): Effectiveness of Different Doses (1982)

In order to compare the effects on gallstone dissolution of three different doses of UDCA (4, 8 and 12 mg/kg/day), a controlled trial was carried out with seventy-one patients. The dissolution rate was higher in the group treated with the highest dose. Stone size was the major factor affecting the outcome of treatment, with a significantly higher success rate in patients with gallstones less than 10 mm than with stones greater than 10 mm.
LR Parsing (1974)

The LR syntax analysis method is a useful and versatile technique for parsing deterministic context-free languages in compiling applications. This paper provides an informal exposition of LR parsing techniques emphasizing the mechanical generation of efficient LR parsers for context-free grammars. Particular attentmn is given to extending the parser generation techniques to apply to ambiguous grammars.
Endogenous brain oscillations and related networks detected by surface EEG‐combined fMRI (2008)

It is difficult to study the brain "at rest" with an approach generally pursued in science when external manipulation (independent variable) is used to obtain informative measurements (dependent variable) about the object of interest. External manipulation in its classic sense may suspend the resting state, and hence the object of interest will evade. Naturally, unless in a final and irreversible state, biological rest will always be an endogenously dynamic process. Combining two modalities, electroencephalography (EEG) and functional magnetic resonance imaging (fMRI), to simultaneously measure the brain's activity from two angles, one can be chosen to be interpreted as the independent variable and the other as the dependent variable, and without external manipulation the brain's spontaneous dynamics can be studied. The EEG, for example, observes endogenous modulations of vigilance and detects spontaneous events such as sleep spindles or epileptic discharges and can be used as the independent variable, i.e., to form a regressor to interrogate the fMRI data (dependent variable). The opposite is possible as well, and data fusion attempts try using all data both as dependent and independent variables at the same time. This review limits itself to an exemplary discussion of simultaneous EEG/fMRI studies in humans, and among a variety of proposed resting state networks only discusses a few, especially those for which non-resting state literature has proposed a functional meaning: the "default mode" network and an attentional network. It will be shown that one EEG feature can correlate with different fMRI activation maps and that a single resting state network may be associated with a variety of EEG patterns giving insight into the function of different resting states and the relationship between the two modalities in themselves.
Allergic rhinitis: not purely a histamine‐related disease (2000)

Allergic rhinitis is an inflammatory disorder of the nasal mucosa typified by the symptoms of nasal itch, sneeze, anterior nasal secretions, and nasal blockage. These symptoms arise from the interaction between mediators and neural, vascular, and glandular structures within the nose. Nasal itch, sneezes, and rhinorrhoea are predominantly neural in origin, while nasal obstruction is predominantly vascular. Nasal biopsy studies show accumulation of eosinophils within the lamina propria and epithelium and an increase in tissue and cell surface basophils in both seasonal and perennial allergic rhinitis. These cells are in an activated state. Within the epithelium, increased numbers of mast cells, T cells and Langerhans' cells, which induce T-cell activation, are found. The accumulation of these cells can be linked to chemokine and cytokine generation by the epithelial cells themselves. Thus, the tissue cell recruitment is orchestrated by activated mast cells, T cells, and epithelial cells, with the recruited tissue eosinophils also contributing to their persistence at this site through autocrine mechanisms. Mast cells generate an array of mediators including histamine, tryptase, leukotrienes, and prostaglandins. Histamine is also generated by basophils. Eosinophils and basophils contribute to the leukotriene synthesis within the tissue. Histamine nasal insufflation induces nasal itch, sneeze, and rhinorrhoea as well as nasal blockage, thereby reproducing all the symptoms of allergic rhinitis. These effects are primarily mediated by H1-receptors, and H1-receptor antagonists are a prominent treatment. Antagonism of histamine at these receptors reduces symptoms by about 40-50%, with the greatest effect on the neurally mediated responses. Thus, histamine is a major mediator of allergic rhinitis, but not the sole contributor. Nasal insufflation with leukotrienes, prostaglandins, or kinins is associated with the development of nasal blockage. These mediators act primarily on the nasal vasculature and, in this respect, leukotrienes are potent mediators. Leukotrienes also induce plasma protein exudation, which contributes to the anterior nasal secretions. Studies with combination products have suggested that modifying the effects of both leukotrienes and histamine has complementary effects in relieving nasal symptoms, indicating that both these mediators are relevant to disease expression.
Habitat Use by Reintroduced Mountain Quail (2017)

Mountain quail (Oreortyx pictus) have declined in much of the Intermountain Region of the western United States. Many areas that once supported these birds now seemingly lack necessary food and cover, especially in critical riparian zones. Additionally, mountain quail appear to need periodic disturbance (fire, moderate grazing, etc.) to provide adequate forage and nesting areas. If mountain quail do not readily occupy suitable habitats, either because of restricted movements or because of habitat discontinuities, it may be necessary to stock birds in order to restore populations. In September 1995, we began a restoration program with the objective of reintroducing mountain quail into former ranges in eastern Oregon and Washington. In the winter of 1996--1997, we released 17 radio-marked birds into a drainage in Hell's Canyon as a pilot study to determine habitat use, survival estimates, and movement patterns. An additional 40 radio-marked birds were released during spring 1998 to determine habitat use, nesting success, and brood survival.
Ultrahigh resolution in vivo versus ex vivo OCT imaging and tissue preservation (2001)

Summary form only given. Many previous studies have compared ex vivo OCT imaging to histopathology. While some tissues, such are arterial pathology or cartilage, are relatively stable post mortem, others, such as epithelial tissues, exhibit rapid degradation. It is therefore important to preserve these tissues with minimal changes in morphology. The goal of this study is to investigate the difference between in vivo and ex vivo OCT imaging and the effect of different preservation solutions on image quality using the hamster cheek pouch. The hamster cheek pouch was chosen because of its easy access and because it is a well established model for carcinogenesis and cancer progression. The advent of the ultrahigh resolution OCT imaging technology is important for this study because it enables changes in tissue morphology to be dearly visualized which may have been difficult to resolve with standard resolution OCT imaging.
Risk Factors for Mortality in Community-Acquired Pneumonia Patients Admitted to a Referral Hospital (2006)

Background: Pneumonia is the most common cause of death among infectious diseases with community-acquired pneumonia being the sixth leading cause of death in the USA. In Korea, several studies have evaluated the prognosis of community-acquired pneumonia with a limited number of patients and risk factors. This study, evaluated all the possible risk factors (including the pneumonia severity index; PSI) in for the community-acquired pneumonia patients admitted to a referral hospital. Methods: The medical records of patients admitted to the Chung-Aug University Yongsan Hospital between January 2002 and January 2005 for community-acquired pneumonia were reviewed retrospectively. The demographic data, comorbidity, radiographic findings and laboratory results which might influence the prognosis of pneumonia were analyzed. Results: Among 179 patients admitted for community-acquired pneumonia, 29 patients died (mortality 16%). The risk factors for mortality in the comorbidity category were congestive heart failure and a myocardial infarction. The laboratory data, showed that albumin, LDH, total cholesterol, HDL, PT, aPTT, hemoglobin and blood urea nitrogen (BUN) were related to the prognosis. For the pneumonia severity index, the mortality rate increased in a step-wise manner from class I through class V.
Predicting Fracture Attributes in the Travis Peak Formation Using Quantitative Mechanical Modeling and Structural Diagenesis (2002)

ABSTRACT Fluid flow prediction in naturally fractured reservoirs is challenging, since fracture pattern attributes such as aperture, filling, spacing, length and connectivity are difficult to obtain from core samples and logs. We combine two methods that circumvent this sampling problem to predict fracture characteristics within the Travis Peak Formation. Firstly, a geomechanical approach is employed to estimate aperture, spacing, length and connectivity of the fracture pattern. This model uses subcritical fracture index, a rock property, and geological boundary conditions as input. Secondly, microfractures are used to estimate conductivity and orientation of macrofractures. The relationship between microfractures and macrofractures has been established in previous studies. Likewise, subcritical fracture index has been shown to influence natural fracture pattern characteristics such as length, spacing and connectivity. However, the dependence of subcritical index on petrologic features is still largely unknown. In this study, suites of subcritical fracture growth experiments were performed on the Travis Peak Formation to investigate systematic variations in subcritical fracture index. Observations show that subcritical fracture index increases with decreasing grain size. Preliminary testing also suggests that subcritical index varies with carbonate cement. Additional tests were carried out in which artificial cement was added to samples. The added cement resulted in increased strength, but decreased subcritical index. We postulate that this kind of artificial cementing of the sample can be equated to secondary carbonate cementation. Using these techniques and understanding the depositional and diagenetic history of a reservoir provides for natural fracture pattern prediction without extensive sampling.
Discriminating Carotid Atherosclerotic Lesion Severity by Luminal Stenosis and Plaque Burden (2010)

Measures of plaque burden do not substantially improve disease assessment compared to stenosis. The finding of IPH in all categories of stenosis and plaque burden suggests that direct characterization of plaque composition and surface status is necessary to fully discriminate disease severity.
Comparative studies of recombinant human albumin and human serum albumin derived by blood fractionation (1996)

Recombinant human albumin produced in the yeast Saccharomyces cerevisial and human serum albumin derived from blood fractionation were compared by a series of analytical techniques. These demonstrated that the two proteins were equivalent structurally. However, differences observed in some of the assays indicated that the recombinant product had lower levels of structural heterogeneity than the blood-derived protein.
TIMP-2 Promotes Activation of Progelatinase A by Membrane-type 1 Matrix Metalloproteinase Immobilized on Agarose Beads (1998)

Membrane-type 1 matrix metalloproteinase (MT1-MMP)/MMP-14 is the activator of progelatinase A (proGelA)/proMMP-2 on the cell surface. However, it was a paradox that a tissue inhibitor of metalloproteinase-2 (TIMP-2), which is an inhibitor of MT1-MMP, is required for proGelA activation by the cells expressing MT1-MMP. In this study, a truncated MT1-MMP having a FLAG-tag sequence at the C terminus (MT1-F) was immobilized onto agarose beads (MT1-F/B) and used to analyze the role of TIMP-2. The proteolytic activity of MT1-F/B against a synthetic peptide substrate was inhibited by TIMP-2 in a dose-dependent manner. In contrast, TIMP-2 promoted the processing of proGelA by MT1-F/B at low concentrations and inhibited it at higher concentrations. TIMP-2 promoted the binding of proGelA to the MT1-F on the beads by forming a trimolecular complex, which was followed by processing of proGelA. A stimulatory effect of TIMP-2 was observed under conditions in which unoccupied MT1-F was still available. Thus, the ternary complex is thought to act as a means to concentrate the substrate to the bead surface and to present it to the neighboring free MT1-F.
Cost-Effectiveness of Second-Generation Antipsychotics and Perphenazine in a Randomized Trial of Treatment for Chronic Schizophrenia (2006)

Treatment with perphenazine was less costly than treatment with second-generation antipsychotics with no significant differences in measures of effectiveness. However, the trial was limited by a high dropout rate, and longer-term neurological and metabolic side effects require further study.
Peptide YY Inhibits Exocrine Pancreatic Secretion in Isolated Perfused Rat Pancreas by Y1 Receptors (1995)

Peptide YY (PYY) inhibits exocrine pancreatic secretion in several species. Two receptors, Y1 and Y2, are known to mediate PYY actions. While PYY 1-36 binds equally to both receptor subtypes, a second endogenous form of PYY, PYY 3-36, selectively activates Y2 receptors. The importance of Y receptor subtypes for inhibition of exocrine pancreatic secretion by PYY is unknown. We studied the effects of PYY 1-36 on cholecystokinin octapeptide (CCK-8)-stimulated amylase secretion in an isolated perfused rat pancreas model. To characterize functionally the receptors involved we determined the effects of a Y1-selective agonist, [Pro34]PYY; a Y2 selective agonist, PYY 3-36; and neuropeptide Y (NPY) in this model. PYY 1-36 significantly inhibited stimulated amylase secretion in the denervated rat pancreas. [Pro34]PYY and NPY both inhibited exocrine pancreatic secretion as potently as PYY 1-36. Contrary to that, the Y2 selective agonist, PYY 3-36, was inactive. We conclude that PYY inhibits exocrine pancreatic secretion in this extrinsically denervated rat pancreas model by Y1 receptors.
Adsorption of aromatic compounds from solutions on titanium dioxide and silica (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAdsorption of aromatic compounds from solutions on titanium dioxide and silicaN. A. Eltekova and Yu. A. EltekovCite this: Langmuir 1987, 3, 6, 951–957Publication Date (Print):November 1, 1987Publication History Published online1 May 2002Published inissue 1 November 1987https://doi.org/10.1021/la00078a012RIGHTS & PERMISSIONSArticle Views123Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (739 KB) Get e-Alerts Get e-Alerts