gebhart/patrepeval huggingface dataset:
Thrones of the Orichas: Afro-Cuban Altars in New Jersey, New York, and Havana (1993)

Apres une description des trones des « orichas » (autels pour deites afro-cubaines d'origine yoruba) trouves a New-York, New-Jersey, Miami, Havana et Matanzas (trones d'initiation et travail de perles, trones pour l'anniversaire d'un saint et trones pour les tambours sacres), les offrandes (essentiellement des fruits et des aliments) et leur echange rituel, l'A. montre la presence des trones de la royaute « oricha » dans l'histoire culturelle afro-cubaine et explique le processus de creolisation des Caraibes (imitations, appropriation, renovation).
Interaction between GABAA Receptor β Subunits and the Multifunctional Protein gC1q-R (2001)

gamma-Aminobutyric acid type A (GABA(A)) receptors were immunopurified from bovine brain using a monoclonal antibody directed against the alpha1 subunit. Of the several proteins that copurified, a 34-kDa protein was analyzed further. After enrichment and tryptic proteolysis, the resulting fragments were sequenced, and the protein was identified as gC1q-R. Using anti-gC1q-R and anti-GABA(A) receptor antibodies, mutual coimmunoprecipitation could be demonstrated from solubilized rat brain membranes. The stability of this interaction was estimated to be very high. Using the yeast two-hybrid system, various GABA(A) receptor subunit intracellular loop constructs were tested for an interaction with gC1q-R. All beta subunits, but not alpha 1 and gamma 2 subunits, were found to bind to gC1q-R. NH(2)- and COOH-terminally truncated beta 2 subunit loops were used to find the region responsible for the interaction with gC1q-R. A stretch of 15 amino acids containing 7 positively charged residues was identified (amino acids 399--413). This region contains residue Ser-410, which is a protein kinase substrate, and it is known that phosphorylation of this residue leads to an alteration in receptor activity. Localization studies suggested a predominantly intracellular localization. Our observations therefore suggest a tight interaction between gC1q-R and the GABA(A) receptor which might be involved in receptor biosynthesis or modulation of the mature function.
Cycloheptatrienylidene analog of 11-cis retinal. Formation of pigment in photoreceptor membranes. (1984)

In biochemical and electrophysiologic studies employing the bullfrog (Rana catesbeiana) and the rat, the authors examined the interaction of opsin and an 11-cis-locked analog of retinal. In previously bleached preparations of bullfrog receptor outer segments (ROS) and isolated retinas, incubation with the aldehyde form (I) of the analog leads to the appearance of a pigment that is degraded slowly by hydroxylamine but is relatively resistant to photolysis. In the ROS preparation, the analog pigment (lambda max of difference spectrum congruent to 497 nm) also forms on incubation with NADP+ and the alcohol form (II) of the analog. In vitamin A deprived rats possessing only approximately 45% of the normal complement of rhodopsin, intraperitoneal injection of II leads within 1 day to the appearance of the analog pigment in the photoreceptors, at levels representing a major fraction of the opsin initially available for pigment formation. Formation of the analog pigment appears to have no significant effect on the sensitivity of electroretinographic b-wave responses recorded from the rat eye; furthermore, administration of II appears to suppress the sensitizing activity of all-trans retinol injected 1 day later. The data are discussed in relation to other studies examining chromophore-opsin interactions and electrophysiologic changes associated with the formation of rhodopsin in situ.
Phase I Trial of Adenovirus-Mediated p53 Gene Therapy for Recurrent Glioma: Biological and Clinical Results (2003)

Intratumoral injection of Ad-p53 allowed for exogenous transfer of the p53 gene and expression of functional p53 protein. However, at the dose and schedule evaluated, transduced cells were only found within a short distance of the injection site. Although toxicity was minimal, widespread distribution of this agent remains a significant goal.
Development of a new fault location system for multi-terminal single transmission lines (1995)

Conventional fault location systems which use one-terminal AC voltages and currents are difficult to apply to multi-terminal power systems. This paper discusses a new fault location system for multi-terminal single transmission lines. Asynchronous sampling at each terminal is preferred in order to simplify the transmission equipment and an algorithm for synchronizing the asynchronous sampling data is presented. Another algorithm is presented which converts the original multi-terminal system by progressively conversion to a system with one fewer terminals to arrive at a 2-terminal system containing the fault. An effective fault locating system can be constructed by combining these algorithms with existing reactive power locating operations. EMTP simulation results are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Diploidization of bovine oocytes matured in vitro and parthenogenetically activated by electric shock. (1990)

Bovine in vitro matured oocytes were parthenogenetically activated by a single pulse of direct current. Suppression of second polar body extrusion by 60 V DC-pulse followed by cytochalasin B treatment or 240 V DC-pulse were used to diploidize parthenogenones. Analysis of the cytological events 20 h postactivation clearly documented high efficiency of both diploidization techniques. All oocytes exposed to a single 60 V DC-pulse and then to cytochalasin B for 3 to 6 h were activated and 70% to 88% possessed two pronuclei. Exposure to 240-V DC-pulse for 10 and 20 microseconds activated all oocytes, 72% and 62% formed two pronuclei, but a high incidence (22% and 30%) of degenerated oocytes was observed. Prolonged in vitro culture of oocytes after parthenogenetic activation and diploidization treatment showed that formed pronuclei were able to fuse and single prometaphase to telophase mitotic cleavage figures developed in all oocytes fixed 28 h after activation, except one cytochalasin B-treated oocyte with two prometaphase sets of chromosomes.
Stimuli-sensitive hydrogels in controlled and sustained drug delivery. (2003)

Recently, controlled and sustained drug delivery has become the standard in modern pharmaceutical design and an intensive research has been undertaken in achieving much better drug product effectiveness, reliability and safety. In this regard, many polymers are very useful with majority of hydrogels, which undergo reversible volume and/or sol-gel phase transitions in response to physiological (temperature, pH and present of ions in organism fluids, blood glucose level) or other external (electric current, light) stimuli. This article reviews the main stimuli-sensitive hydrogels and the use of these hydrogels in parenteral, ocular, peroral, rectal, vaginal, nasal, dermal and transdermal drug delivery.
The Barnett Shale Play, Fort Worth Basin: Abstract (2003)

Abstract In terms of monthly production, the Newark East (Barnett Shale) field recently became the largest gas field in Texas. Production has grown from 80 MMCF/D in January 2000 to over 560 MMCF/D at present because of accelerated new-well drilling and old-well reworks/refracs. There are over 2.5 TCF of booked proven gas reserves in the field at present. Newark East field is located in the northern portion of the Fort Worth Basin, just north of the city of Fort Worth. The Mississippian Barnett rests on an extensive angular unconformity. The Barnett must be stimulated to achieve economic flow rates. Currently, wells are hydraulically fractured, but good frac barriers must be present directly above and below the Barnett for this stimulation technique to be successful. Hence, the stratigraphy above and below the Barnett is important to economic production. The thermal history of the basin is an important reason for the success of the Barnett. The thermal history of the Fort Worth basin is directly related to the emplacement of the Ouachita system. In the late 1990s, work by Mitchell Energy had demonstrated the viability of water fracs in the Barnett play. Also in the late 1990s, Mitchell determined that the previous gas-in-place values for the Barnett were too low; there is approximately 150 BCF/mi2 of in-place gas. The success of the Barnett play may provide a model for prospecting for other large shale-reservoirs.
Dynamic pharming attacks and locked same-origin policies for web browsers (2007)

We describe a new attack against web authentication, which we call dynamic pharming. Dynamic pharming works by hijacking DNS and sending the victim's browser malicious Javascript, which then exploits DNS rebinding vulnerabilities and the name-based same-origin policy to hijack a legitimate session after authentication has taken place. As a result, the attack works regardless of the authentication scheme used. Dynamic pharming enables the adversary to eavesdrop on sensitive content, forge transactions, sniff secondary passwords, etc. To counter dynamic pharming attacks, we propose two locked same-origin policies for web browsers. In contrast to the legacy same-origin policy, which regulates cross-object access control in browsers using domain names, the locked same-origin policies enforce access using servers' X.509 certificates and public keys. We show how our policies help two existing web authentication mechanisms, client-side SSL and SSL-only cookies, resist both pharming and stronger active attacks. Also, we present a deployability analysis of our policies based on a study of 14651 SSL domains. Our results suggest one of our policies can be deployed today and interoperate seamlessly with the vast majority of legacy web servers. For our other policy, we present a simple incrementally deployable opt-in mechanism for legacy servers using policy files, and show how web sites can use policy files to support self-signed and untrusted certificates, shared subdomain objects, and key updates.
Role of Immune Responses against the Envelope and the Core Antigens of Simian Immunodeficiency Virus SIVmne in Protection against Homologous Cloned and Uncloned Virus Challenge in Macaques (1999)

ABSTRACT We previously showed that envelope (gp160)-based vaccines, used in a live recombinant virus priming and subunit protein boosting regimen, protected macaques against intravenous and intrarectal challenges with the homologous simian immunodeficiency virus SIVmne clone E11S. However, the breadth of protection appears to be limited, since the vaccines were only partially effective against intravenous challenge by the uncloned SIVmne. To examine factors that could affect the breadth and the efficacy of this immunization approach, we studied (i) the effect of priming by recombinant vaccinia virus; (ii) the role of surface antigen gp130; and (iii) the role of core antigens (Gag and Pol) in eliciting protective immunity. Results indicate that (i) priming with recombinant vaccinia virus was more effective than subunit antigen in eliciting protective responses; (ii) while both gp130 and gp160 elicited similar levels of SIV-specific antibodies, gp130 was not as effective as gp160 in protection, indicating a possible role for the transmembrane protein in presenting functionally important epitopes; and (iii) although animals immunized with core antigens failed to generate any neutralizing antibody and were infected upon challenge, their virus load was 50- to 100-fold lower than that of the controls, suggesting the importance of cellular immunity or other core-specific immune responses in controlling acute infection. Complete protection against intravenous infection by the pathogenic uncloned SIVmne was achieved by immunization with both the envelope and the core antigens. These results indicate that immune responses to both antigens may contribute to protection and thus argue for the inclusion of multiple antigens in recombinant vaccine designs.
The effects of host genotype, growth rate, and needle age on the distribution of a mutualistic, endophytic fungus in Douglas-fir plantations (1988)

Two young plantations of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) were examined for the presence of a symptomless endophytic fungus, Meriaparkeri Sherwood-Pike (Hemiphacideaceae). This fungus occurred in the needles of nearly every tree examined, with infection frequencies increasing with the age of the needle. Previous reports have asserted that M. parkeri is a mutualist that produces insecticidal toxins. The maternal parentage of the trees and age of the needles were highly significant predictors of infection frequencies. A detailed examination of two families of trees revealed that infection frequencies differed between sites and were positively correlated with the height of the host. Mutualism and microhabitat effects are discussed.
Heterocalixarenes. 1. Calix[2]uracil[2]arene:  Synthesis, X-ray Structure, Conformational Analysis, and Binding Character (1999)

1,3-Bis[(1-uracilyl)methyl)]benzene derivatives 3, formed by selective N-1 alkylation of 1,3-bis(trimethylsilyloxy)pyrimidine with 1,3-bis(bromomethyl)benzene derivatives 2, on cyclization with the same or different derivative of 2, provide calix[2]uracil[2]arenes 4. Their conformations, investigated through X-ray, variable temperature 1H NMR, and molecular modeling MM2 calculations, are found to depend on the nature of substituent(s) on the position 2 of 1,3-phenylene rings. 4a exists in equilibrium between various conformations, and 4b, 4d−4i adopt an inward flattened partial cone conformation. In 4j, the equilibrium is restricted to two conformations at −60 °C, but the 4j·ethanol complex (2:1), in the solid state, has a cone conformation with ethanol being a H-bond donor through H of OH and CH2 and an acceptor through O of OH. 4d, 4j, and 4k exhibit significant preferences toward alkaline earth metal ions over alkali metal ions though overall bindings are poor.
Synthesis of 3′-Thioribouridine, 3′-Thioribocytidine, and Their Phosphoramidites (1997)

Abstract 3′-Thio-3′-deoxyribonucleosides (U and C) have been synthesized via Vorbruggen-type glycosylation with 3-S-benzoyl-5-O-toluoyl-1,2-O-diacetylfuranose, which was obtained from 1,2-O-isopropylidene-5-O-toluoyl-3-O-trifluoromethanesulfonyl-α-D-xylofuranose. 3′-Thio-3′-deoxyuridine has been converted to its phosphoramidite. Notes Every new compound was characterized by 1H- and 13C-NMR, and either a satisfictory molecular weight from high resolution mass spectrometry or a satisfactory elemental analysis was obtained. For the final thiouridine phosphoramidite 14 31 P-NMR (90 MHz, in CDCI3, using 85% H3PO4 as external standard): = 168.6, 157.4; Anal. calcd for C45H61N4O8PSSi: C, 61.64; H, 6.96; N, 6.39; S, 3.65. Found: C, 61.38; H, 6.95; N, 6.62; S, 3.49.
Characterization of Ca2+-dependent Phospholipase A2 Activity during Zebrafish Embryogenesis (1999)

We have developed a simple fluorescent assay for detection of phospholipase A2 (PLA2) activity in zebrafish embryos that utilizes a fluorescent phosphatidylcholine substrate. By using this assay in conjunction with selective PLA2 inhibitors and Western blot analysis, we identified the principal activity in zebrafish embryogenesis as characteristic of the Ca2+-dependent cytosolic PLA2 (cPLA2) subtype. Embryonic cPLA2 activity remained constant from the 1-cell stage until the onset of somitogenesis, at which time it increased sharply. This increase was preceded by the expression of a previously identified zebrafish cPLA2 homologue (Nalefski, E., Sultzman, L., Martin, D., Kriz, R., Towler, P., Knopf, J., and Clark, J. (1994) J. Biol. Chem. 269, 18239-18249). By using a quenched BODIPY-labeled phosphatidylcholine that fluoresces only upon cleavage by PLA2, lipase activity was visualized in the cells of living embryos where it localized to perinuclear membranes.
Long-term Retinal Function and Structure Rescue Using Capsid Mutant AAV8 Vector in the rd10 Mouse, a Model of Recessive Retinitis Pigmentosa (2010)

The retinal degeneration 10 (rd10) mouse is a well-characterized model of autosomal recessive retinitis pigmentosa (RP), which carries a spontaneous mutation in the β subunit of rod cGMP-phosphodiesterase (PDEβ). Rd10 mouse exhibits photoreceptor dysfunction and rapid rod photoreceptor degeneration followed by cone degeneration and remodeling of the inner retina. Here, we evaluate whether gene replacement using the fast-acting tyrosine-capsid mutant AAV8 (Y733F) can provide long-term therapy in this model. AAV8 (Y733F)-smCBA-PDEβ was subretinally delivered to postnatal day 14 (P14) rd10 mice in one eye only. Six months after injection, spectral domain optical coherence tomography (SD-OCT), electroretinogram (ERG), optomotor behavior tests, and immunohistochemistry showed that AAV8 (Y733F)-mediated PDEβ expression restored retinal function and visual behavior and preserved retinal structure in treated rd10 eyes for at least 6 months. This is the first demonstration of long-term phenotypic rescue by gene therapy in an animal model of PDEβ-RP. It is also the first example of tyrosine-capsid mutant AAV8 (Y733F)-mediated correction of a retinal phenotype. These results lay the groundwork for the development of PDEβ-RP gene therapy trial and suggest that tyrosine-capsid mutant AAV vectors may be effective for treating other rapidly degenerating models of retinal degeneration.
Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1. (1996)

The Bcl-2 protein blocks programmed cell death (apoptosis) through an unknown mechanism. Previously we identified a Bcl-2 interacting protein BAG-1 that enhances the anti-apoptotic effects of Bcl-2. Like BAG-1, the serine/threonine protein kinase Raf-1 also can functionally cooperate with Bcl-2 in suppressing apoptosis. Here we show that Raf-1 and BAG-1 specifically interact in vitro and in yeast two-hybrid assays. Raf-1 and BAG-1 can also be coimmunoprecipitated from mammalian cells and from insect cells infected with recombinant baculoviruses encoding these proteins. Furthermore, bacterially-produced BAG-1 protein can increase the kinase activity of Raf-1 in vitro. BAG-1 also activates this mammalian kinase in yeast. These observations suggest that the Bcl-2 binding protein BAG-1 joins Ras and 14-3-3 proteins as potential activators of the kinase Raf-1.
Digital array scanned interferometer: sensors and results (1996)

Digital array scanned interferometers (DASI) blend characteristics of a grating spectrometer and a two-beam interferometer for acquisition of hyperspectra. DASI's posses field-widened capabilities that permit very high throughput. Aspects of DASI design, hyperspectra, and data processing methods are presented. In particular, we provide data showing that photon-noise-limited hyperspectra are achievable for DASI data.
Load balancing policies in heterogeneous distributed systems (2002)

This paper presents a new load balancing policy in heterogeneous distributed systems. All nodes in this system connected in point-to-point may have different service rates, and each node can serve not only external arrival tasks but also the tasks migrated from the other nodes. The scheduler takes into account the threshold value, based on the ratio of service rates, and the current queue length in determining whether one task shall be migrated to the other node or left for the local node. A Markov process model is used to describe the behavior of the heterogeneous distributed system under the proposed policies. The model is then solved by using the matrix-geometric solution technique. Performance of the policy is compared to that of the generic load balancing policy, no load balancing policy, and the ideal load balancing policy. The results show that the proposed policy is better and closer to the ideal load balancing policy.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Oxygen Inhibits Neuronal Activation in the Trigeminocervical Complex After Stimulation of Trigeminal Autonomic Reflex, But Not During Direct Dural Activation of Trigeminal Afferents (2009)

The data provide the first systematic, experimental evidence for a mechanism of action of oxygen in cluster headache. The data show oxygen has no direct effect on trigeminal afferents, acting specifically on the parasympathetic/facial nerve projections to the cranial vasculature to inhibit both evoked trigeminovascular activation and activation of the autonomic pathway during cluster headache attacks. Moreover, the studies begin to characterize a novel laboratory model for the most painful primary headache syndrome known--cluster headache.
Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro. (1975)

NAD is a necessary cofactor for the activation of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by cholera toxin. Lysates of certain types of cell that hydrolyze their endogenous store of NAD after cell disruption respond poorly or not at all to cholera toxin. Lysates of pigeon erythrocytes, which lack enzymes that degrade NAD, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action of the toxin upon broken cells.
Integrated face and gait recognition from multiple views (2005)

We develop a view-normalization approach to multi-view face and gait recognition. An image-based visual hull (IBVH) is computed from a set of monocular views and used to render virtual views for tracking and recognition. We determine canonical viewpoints by examining the 3D structure, appearance (texture), and motion of the moving person. For optimal face recognition, we place virtual cameras to capture frontal face appearance; for gait recognition we place virtual cameras to capture a side-view of the person. Multiple cameras can be rendered simultaneously, and camera position is dynamically updated as the person moves through the workspace. Image sequences from each canonical view are passed to an unmodified face or gait recognition algorithm. We show that our approach provides greater recognition accuracy than is obtained using the unnormalized input sequences, and that integrated face and gait recognition provides improved performance over either modality alone. Canonical view estimation, rendering, and recognition have been efficiently implemented and can run at near real-time speeds.