gebhart/patrepeval huggingface dataset:
Use of Young Nude Mice for Selection of Subpopulations of Cells With Increased Metastatic Potential From Nonsyngeneic Neoplasms<xref ref-type="fn" rid="FN2">2</xref><xref ref-type="fn" rid="FN3">3</xref><xref ref-type="fn" rid="FN4">4</xref> (1982)

The purpose of these studies was to investigate whether young (3-wk-old) nude mice, which lack functional T-lymphocytes and demonstrate low natural killer cell activity, could serve as in vivo models for the selection of metastatic subpopulations of cells from heterogeneous allogeneic melanomas. Three-week-old BALB/cAnN or N:NIH(S) nude mice received iv injections of single cells harvested from either the B16 or K-1735 melanoma. Individual pulmonary metastases were harvested 4 weeks later and implanted sc into nude mice to expand the population. Cells from each of these metastases colonized in the lungs of 3-week-old nude mice and 6-week-old normal syngeneic mice with significantly higher efficiency than did cells from the parent tumors. It was concluded that, in addition to being a useful in vivo model for ascertaining the metastatic potential of neoplasms, the healthy young nude mouse could be used for selecting and maintaining tumor cell variants of high metastatic potential room heterogeneous allogeneic tumors.
PENERAPAN UPAH MINIMUM REGIONAL TAHUN 2016 DALAM MENENTUKAN TARIF JASA TEKNOLOGI DESAIN DULANG KERAMIK (2017)

The aims of this research that relate regional minimum wage 2016 intodetermine technology service rate ceramics coaster design were: (1) to know theinfluence of regional minimum wage to cost of goods manufactured; (2) to know thecomparisson of servive rate (cost price) year 2015 and 2016; and (3) to know theprofitability year 2015 and 2016. The research results shew that: (1) the addition ofregional minimum wage Denpasar Region can increase cost of good manufacturedceramics coaster design. (2) The comparisson of service rate (cost price) ceramiccoaster design in 2015 different with 2016. The comparisson of service rate ceramiccoaster design in 2016 to 2015 was 1,07; and (3) The addition of regional minimumwage Denpasar Region in 2016 can increase profitability ((Gross Profit Margin(GPM), Operating Profit Margin (OPM), and Net Profit Margin (NPM)) service rateceramic coaster design. The Gross Profit Margin (GPM), Operating Profit Margin(OPM), and Net Profit Margin (NPM)) in 2016 were Rp 2.348,14, Rp 1.174,07, andRp 1.018,33 each unit. It were mean that if the minimum wage of Denpasar regionincrease, so the profitability ((Gross Profit Margin (GPM, Operating Profit Margin(OPM), and Net Profit Margin (NPM)) tends will increase.
Long-term Correction of Very Long-chain Acyl-CoA Dehydrogenase Deficiency in Mice Using AAV9 Gene Therapy (2012)

Very long-chain acyl-coA dehydrogenase (VLCAD) is the rate-limiting step in mitochondrial fatty acid oxidation. VLCAD-deficient mice and patients clinical symptoms stem from not only an energy deficiency but also long-chain metabolite accumulations. VLCAD-deficient mice were treated systemically with 1 × 10(12) vector genomes of recombinant adeno-associated virus 9 (rAAV9)-VLCAD. Biochemical correction was observed in vector-treated mice beginning 2 weeks postinjection, as characterized by a significant drop in long-chain fatty acyl accumulates in whole blood after an overnight fast. Changes persisted through the termination point around 20 weeks postinjection. Magnetic resonance spectroscopy (MRS) and tandem mass spectrometry (MS/MS) revealed normalization of intramuscular lipids in treated animals. Correction was not observed in liver tissue extracts, but cardiac muscle extracts showed significant reduction of long-chain metabolites. Disease-specific phenotypes were characterized, including thermoregulation and maintenance of euglycemia after a fasting cold challenge. Internal body temperatures of untreated VLCAD(-/-) mice dropped below 20 °C and the mice became lethargic, requiring euthanasia. In contrast, all rAAV9-treated VLCAD(-/-) mice and the wild-type controls maintained body temperatures. rAAV9-treated VLCAD(-/-) mice maintained euglycemia, whereas untreated VLCAD(-/-) mice suffered hypoglycemia following a fasting cold challenge. These promising results suggest rAAV9 gene therapy as a potential treatment for VLCAD deficiency in humans.
The C-Type Lectin Macrophage Galactose-Type Lectin Impedes Migration of Immature APCs (2008)

Dendritic cells (DCs) are the most potent APCs of the immune system that seed the peripheral tissues and lymphoid organs. In an immature state, DCs sample their surroundings for incoming pathogens. Upon Ag encounter, DCs mature and migrate to the lymph node to induce adaptive immune responses. The C-type macrophage galactose-type lectin (MGL), expressed in immature DCs, mediates binding to glycoproteins carrying GalNAc moieties. In the present study, we demonstrate that MGL ligands are present on the sinusoidal and lymphatic endothelium of lymph node and thymus, respectively. MGL binding strongly correlated with the expression of the preferred MGL ligand, alpha-GalNAc-containing glycan structures, as visualized by staining with the alpha-GalNAc-specific snail lectin Helix pomatia agglutinin. MGL(+) cells were localized in close proximity of the endothelial structures that express the MGL ligand. Strikingly, instead of inducing migration, MGL mediated retention of human immature DCs, as blockade of MGL interactions enhanced DC trafficking and migration. Thus, MGL(+) DCs are hampered in their migratory responses and only upon maturation, when MGL expression is abolished; these DCs will be released from their MGL-mediated restraints.
Effect of surface treatment and sterilization processes on the corrosion behavior of NiTi shape memory alloy (2000)

Nickel-titanium (NiTi) alloy derives its biocompatibility and good corrosion resistance from a homogeneous oxide layer mainly composed of TiO(2), with a very low concentration of nickel. In this article, we described the corrosion behavior of NiTi alloys after mechanical polishing, electropolishing, and sterilization processes using cyclic polarization and atomic absorption. As a preparative surface treatment, electropolishing decreased the amount of nickel on the surface and remarkably improved the corrosion behavior of the alloy by increasing the mean breakdown potential value and the reproducibility of the results (0.99 +/- 0.05 V/SCE vs. 0.53 +/- 0. 42). Ethylene oxide and Sterrad(R) sterilization techniques did not modify the corrosion resistance of electropolished NiTi, whereas a steam autoclave and, to a lesser extent, peracetic acid sterilization produced scattered breakdown potential. In comparing the corrosion resistance of common biomaterials, NiTi ranked between 316L stainless steel and Ti6A14V even after sterilization. Electropolished NiTi and 316L stainless-steel alloys released similar amounts of nickel after a few days of immersion in Hank's solution. Measurements by atomic absorption have shown that the amount of released nickel from passive dissolution was below the expected toxic level in the human body. Auger electron spectroscopy analyses indicated surface contamination by Ca and P on NiTi during immersion, but no significant modification in oxide thickness was observed.
Treatment of Antibody-Mediated Pure Red-Cell Aplasia with High-Dose Intravenous Gamma Globulin (1987)

PURE red-cell aplasia is a syndrome characterized by severe anemia, reticulocytopenia, and an absence of erythroid precursor cells in bone marrow that is otherwise normal. This failure of erythropoiesis has been shown to be antibody-mediated in almost half the patients with the adult form of red-cell aplasia.1 2 3 4 Immunosuppressive agents such as corticosteroids, cyclophosphamide, and antithymocyte globulin have been used to treat immunologically mediated red-cell aplasia.2 , 5 6 7 8 Results of in vitro assays for hematopoietic progenitor cells may assist in predicting which patients will respond to immunosuppressive therapy.9 10 11 Red-cell aplasia in children usually differs from the disorder in adults. There is a constitutional . . .
Age, gender, and bone lamellae elastic moduli (2000)

To enhance preventative and therapeutic strategies for metabolic bone diseases and bone fragility disorders, we began to explore the physical properties of bone tissue at the cellular level. Proximal femurs were harvested from 27 cadavera (16 male and 11 female) for in vitro measurement of the mechanical properties. We measured the variations in lamellar-level elastic modulus and hardness in human bone as a function of age and gender to identify microstructural properties responsible for age and gender-related reductions in the mechanical integrity. The lateral femoral necks were examined, and age, gender, height, body mass, and body mass index were not found to correlate with lamellar-level elastic modulus or hardness. This result was consistent for osteonal, interstitial, and trabecular tissue. These data suggest that increased bone mass maintenance, known to occur in heavier individuals, is not accompanied by increases in the lamellar-level elastic modulus or hardness. The independence of elastic modulus and hardness from age and gender suggests that age and gender-related decreases in mechanical integrity do not involve alterations in elastic modulus or hardness of the extracellular matrix. Lamellar-level ultimate, fatigue, and fracture toughness properties should also be investigated. Other factors, such as tissue mass and organization, may also contribute to age and gender-related decreases in the mechanical integrity.
Nuclear matrix proteins in human coloncancer. (1994)

The nuclear matrix is the nonchromatin scaffolding of the nucleus. This structure confers nuclear shape, organizes chromatin, and appears to contain important regulatory proteins. Tissue specific nuclear matrix proteins have been found in the rat, mouse, and human. In this study we compared high-resolution two-dimensional gel electropherograms of nuclear matrix protein patterns found in human colon tumors with those from normal colon epithelia. Tumors were obtained from 18 patients undergoing partial colectomy for adenocarcinoma of the colon and compared with tissue from 10 normal colons. We have identified at least six proteins which were present in 18 of 18 colon tumors and 0 of 10 normal tissues, as well as four proteins present in 0 of 18 tumors and in 10 of 10 normal tissues. These data, which corroborate similar findings of cancer-specific nuclear matrix proteins in prostate and breast, suggest that nuclear matrix proteins may serve as important markers for at least some types of cancer.
Angiotensinogen and Its Cleaved Derivatives Inhibit Angiogenesis (2002)

The members of the serine protease inhibitor (serpin) family, which share a common tertiary structure and a role as serin protease inhibitors, are involved in a variety of newly discovered functions. For example, antithrombin III exerts a strong antiangiogenic activity. Angiotensinogen, the renin substrate, has a folded structure and is a member of the noninhibitory serpin subfamily. Two other noninhibitory serpins, maspin and pigment epithelium-derived factor, have antiangiogenic properties. We investigated the antiangiogenic effect of angiotensinogen and 2 related compounds: (1) des(angiotensin I)angiotensinogen, the product of angiotensinogen cleavage by renin, and (2) the reactive center loop-cleaved angiotensinogen, which is produced after selective and limited proteolysis by the protease V8. We used well-established in vitro (endothelial cell proliferation and migration, and capillary-like tube formation on Matrigel) and in vivo (the chick chorioallantoic membrane assay) models of angiogenesis to evaluate the antiangiogenic activities of these 3 related molecules. Our data demonstrated that these compounds exerted a clear and equipotent antiangiogenic effect, thus attributing a novel function to angiotensinogen and des(angiotensin I)angiotensinogen, for which no function was previously known.
18.3: Invited Paper: Research on 7.56‐inch Foldable AMOLED and Relevant Foldable Technologies (2019)

A foldable 7.56‐inch display is introduced. According to the test results of the device, several key elements such as folding performance, pencil hardness, impact resistance and deformation, which might affect the foldable display into the market are discussed.
Gene Therapy of Metastatic Pancreas Cancer with Intraperitoneal Injections of Concentrated Retroviral Herpes Simplex Thymidine Kinase Vector Supernatant and Ganciclovir (1996)

Results demonstrate that IP administration of concentrated retroviral HS-tk vectors is effective treatment for pancreas cancer metastatic to the peritoneal cavity; furthermore, the vector is active in the presence of ascitic fluid. Intraperitoneal retroviral HS-tk may provide a novel approach to treatment of metastatic pancreas cancer.
Insights into the molecular mechanisms of bradycardia-triggered arrhythmias in long QT-3 syndrome (2002)

Congenital long QT syndrome is a rare disease in which the electrocardiogram QT interval is prolonged due to dysfunctional ventricular repolarization.Variant 3 (LQT-3) is associated with mutations in SCN5A, the gene coding for the heart Na + channel subunit.Arrhythmias in LQT-3 mutation carriers are more likely to occur at rest, when heart rate is slow.Several LQT-3 Na + channel mutations exert their deleterious effects by promoting a mode of Na + channel gating wherein a fraction of channels fails to inactivate.This gating mode, termed "bursting, " results in sustained macroscopic inward Na + channel current (I sus ), which can delay repolarization and prolong the QT interval.However, the mechanism of heart-rate dependence of I sus has been unresolved at the single-channel level.We investigate an LQT-3 mutant (Y1795C) using experimental and theoretical frameworks to elucidate the molecular mechanism of I sus rate dependence.Our results indicate that mutation-induced changes in the length of time mutant channels spend bursting, rather than how readily they burst, determines I sus inverse heart-rate dependence.These results link mutation-induced changes in Na + channel gating mode transitions to heart rate-dependent changes in cellular electrical activity underlying a key LQT-3 clinical phenotype.
Determination of VITA Classical shades with the 3D-Master shade guide (2012)

Within the limitations of the study, it is usually possible to determine tooth color with the 3D and convert it, via a table, into a VC shade without adding a clinically apparent error to the direct shade match with the VC.
A Study on Development of Science Education Modules (1990)

Development of science education curriculum and instructional materials have been considered as the matt essential task for the establishment of scince education as an independent discipline, and for the intrenchment of status and identity of colles of Education The aim of this study was to identify the necessity and types for the development of science education curriculum and instructional materials. The nature and structure of science education were discussed in connection with school science subjects, science of education, and the structure of science education system. Also essential theory and practice of modular approach for development of curriculum were investigated in order to organize a guideline for the development of scince education modules. The guideline includes development procedures, major contents. and structure of science education modules which is organized by integration of the nature of scince education and concepts of modular approach for curriculum development.
Quantitative analysis of melanoma-associated antigen p97 in normal and neoplastic tissues. (1981)

We have used two highly sensitive assays to quantitate p97, a protein associated with human melanoma, in cultured cells and normal adult, fetal, and neoplastic tissues. To measure p97 at the surface of intact cells, radiolabeled Fab fragments of a monoclonal antibody specific for p97 were used in a binding assay. To measure p97 in detergent-solubilized membrane preparations, we used a novel double-determinant immunoassay that uses two monoclonal antibodies to two distinct antigenic determinants of p97. These assays revealed that although p97 is present in small amounts in normal adult tissues, it is present in much larger amounts in most melanomas, in some other tumors (both benign and malignant), and in certain fetal tissues. We conclude that monoclonal antibodies to p97 may prove to be of value for the diagnosis and therapy of melanoma.
Feasibility of Electrospinning the Globular Proteins Hemoglobin and Myoglobin (2006)

Various concentrations of the globular protein hemoglobin were successfully electrospun to create micro-fibrous mats of varying physical and mechanical characteristics. The electrospinning parameters are reported. One concentration of myoglobin was electrospun into a mat for comparison to the hemoglobin mats. Scanning electron microscopy revealed ribbon-like morphologies for the hemoglobin and myoglobin structures. Mean fiber width and thickness for each mat electrospun from a different hemoglobin concentration increased from 2.68 ± 0.83 to 3.55 ± 1.49 μm and from 0.49 ± 0.08 to 0.99 ± 0.41 μm, respectively, for increasing hemoglobin solution concentrations (from 150 to 225 mg/mL). For calculations of surface area to volume ratio for the four different electrospun hemoglobin concentrations, there was a negative correlation (r = −0.84) with concentration; the surface area to volume ratio ranged between 0.50 ± 0.16 and 1.53 ± 0.24 m 2 /cm 3 . Also, there appears to be a positive correlation between electrospun hemoglobin concentration and porosity, which increased with increasing concentration from 69.5 to 83.3 %. Following cross-linking with glutaraldehyde, the mechanical properties of two constructs were evaluated via uniaxial tensile testing to demonstrate handling capability. Results indicated that increased cross-linking time produced stiffer structures, as peak stress and modulus increased while strain at break decreased when the mats were cross-linked for 30 minutes with glutaraldehyde versus the 20 minute cross-linking time.
Microbial removal of acetate selectively from sugar mixtures (2011)

Acetic acid is an unavoidable constituent of the biomass hydrolysates generated from acetylated hemicellulose and lignin, and acetate affects the performance of microbes used to convert these hydrolysates into biofuels or other biochemicals. In this study, acetate was selectively removed from synthetic mixtures of glucose and xylose using metabolically engineered Escherichia coli strains having mutations in the glucose phosphotransferase system (PTS) genes (ptsG, manZ, crr), glucokinase (glk), and xylose (xylA). In batch culture, ALS1060 (ptsG manZ glk xylA) consumed exclusively acetate to depletion, and then consumed the two sugars only at a very slow rate (a growth rate of about 0.01 h(-1)). We also examined the effects of an additional knockout of either malX, fruA, fruB, bglF, or crr, genes that are involved in other PTSs, and a batch process using KD840 (ptsG manZ glk crr xylA) demonstrated a further reduction in glucose or xylose consumption by E. coli. These results demonstrate the feasibility of using a substrate-selective approach for the pre-treatment of biomass hydrolysate for microbial processes.
Apolipoprotein E ε4 Allele Is Associated with Vascular Dementia (2006)

This interaction with hypertension and diabetes should be examined in other studies to confirm or refute this observation.
Leukocyte Changes Associated with Acute Inflammation in Chickens (1988)

Leukocyte changes in chickens with turpentine-induced inflammation were investigated sequentially at 0, 6, 12, and 24 hours and at 2, 3, 4, 7, and 14 days. During acute inflammation, significant leukocytosis and heterophilia developed by 6 hours and persisted through 7 days. The peak mean heterophil and leukocyte counts occurred at 12 hours and 3 days, respectively. Left shifts were present at 12 and 24 hours as detected by 100-cell leukocyte differential counts. Heterophil mean nuclear scores documented nuclear hyposegmentation (left shift) during early inflammation and nuclear hypersegmentation (right shift) during convalescence. Mean monocyte and lymphocyte counts peaked at 2 and 3 days, respectively. Basophil and eosinophil counts were erratic. Toxic changes of heterophils were most apparent during intense left shifts and consisted of cell swelling, degranulation, cytoplasmic vacuolation, and cytoplasmic basophilia. Cytoplasmic basophilia was the last aspect of toxic change to resolve. Ultrastructurally, toxic heterophils had intracellular edema, dissolution of granules, retention of ribosomes, nuclear membrane blebs, and decreased heterochromatin density. All inflammation-associated alterations in cell counts and morphology returned to baseline values and appearance by 14 days after turpentine administration.
Increased Polyamine Intake Inhibits Age-Associated Alteration in Global DNA Methylation and 1,2-Dimethylhydrazine-Induced Tumorigenesis (2013)

Polyamines (spermine and spermidine) play many important roles in cellular function and are supplied from the intestinal lumen. We have shown that continuous high polyamine intake inhibits age-associated pathologies in mice. The mechanism by which polyamines elicit these effects was examined. Twenty-four week old Jc1:ICR male mice were fed one of three experimental chows containing different polyamine concentrations. Lifetime intake of high polyamine chow, which had a polyamine content approximately three times higher than regular chow, elevated polyamine concentrations in whole blood, suppressed age-associated increases in pro-inflammatory status, decreased age-associated pathological changes, inhibited age-associated global alteration in DNA methylation status and reduced the mortality in aged mice. Exogenous spermine augmented DNA methyltransferase activity in Jurkat and HT-29 cells and inhibited polyamine deficiency-induced global alteration in DNA methylation status in vitro. In addition, increased polyamine intake was associated with a decreased incidence of colon tumors in BALB/c mice after 1,2-demethylhydrazine administration; 12 mice (60%) in the low polyamine group developed tumors, compared with only 5 mice (25%) in the high polyamine group (Fisher's exact probability = 0.027, p = 0.025). However, increased polyamine intake accelerated the growth of established tumors; maximal tumor diameter in the Low and High groups was 3.85±0.90 mm and 5.50±1.93 mm, respectively (Mann-Whitney test, p = 0.039). Spermine seems to play important roles in inhibiting age-associated and polyamine-deficient induced abnormal gene methylation as well as pathological changes including tumorigenesis.
Horizontal transfer of an exopolymer complex from one bacterial species to another (2000)

Alasan, the exocellular polymeric emulsifier produced by Acinetobacter radioresistens KA53 was shown to bind to the surface of Sphingomonas paucimobilis EPA505 and Acinetobacter calcoaceticus RAG-1. The presence of alasan on the surface of S. paucimobilis EPA505 and A. calcoaceticus RAG-1 caused a decrease in their cell-surface hydrophobicities. Binding was proportional to the concentration of recipient cells and input alasan. At the highest concentration of A. calcoaceticus RAG-1 (4 x 10(9) ml(-1)) and alasan (20 microg ml(-1)) tested, 75% of the alasan was cell bound. Alasan binding was measured by the loss of emulsifying activity and alasan protein and polysaccharide from the aqueous phase after incubation of alasan with the recipient cells. In addition, alasan was visualized on the surface of the recipient cells by staining with anti-alasan antibodies and rhodamine-labelled secondary antibodies. Moreover, when the alasan-producing A. radioresistens KA53 was grown together with A. calcoaceticus RAG-1, alasan was released from the producing strain and became bound to the recipient RAG-1 cells, as demonstrated by fluorescence microscopy. This horizontal transfer of exopolymers from one bacterial species to another has significant implications in natural microbial communities, coaggregation and biofilms.