gebhart/patrepeval huggingface dataset:
Dynamic VPN communities: implementation and experience (2002)

Dynamic communities of independent organizations require fluid collaborative sharing of information resources within member networks. This presents challenging network security problems. The security postures of member networks must rapidly evolve in response to the addition or removal of community members, in order to grant privileged access to members while protecting critical resources and communications from nonmembers. The member networks belong to different administrative domains, and must preserve their autonomy despite the necessary relaxing of access restrictions. This paper describes the design and implementation of a system supporting Dynamic Virtual Private Network (DVPN) communities of independently administered, firewall-protected enclaves. A central authority defines the community, and member enclaves adapt automatically and rapidly as enclaves are admitted or expelled, avoiding the high administrative cost and risk of misconfiguration associated with common VPN mechanisms.
Barriers to effective insulin treatment: the persistence of poor glycemic control in type 2 diabetes (2011)

Although innovations in injectable therapy for T2D may help address the current pattern of poor glycemic control, improved communication between patients and caregivers is also a powerful approach and can be implemented with existing therapies.
Chronic Administration of Belimumab, a BLyS Antagonist, Decreases Tissue and Peripheral Blood B-Lymphocyte Populations in Cynomolgus Monkeys: Pharmacokinetic, Pharmacodynamic, and Toxicologic Effects (2006)

The tolerability, pharmacodynamic effects, and pharmacokinetics of belimumab (LymphoStat-B) were evaluated in cynomolgus monkeys. Belimumab is a fully human IgG1lambda antibody directed against B-lymphocyte stimulator (BLyS) protein. BLyS is a TNF family member that supports B-lymphocyte maturation and survival and has been implicated in the pathogenesis of autoimmune diseases and B-lymphocyte malignancies. Belimumab was developed to antagonize BLyS activity in autoimmune diseases and B-lymphocyte malignancies, where undesirable effects of B-lymphocyte activity may cause or contribute to disease. Pharmacodynamic effects of belimumab were monitored by immunophenotyping of peripheral blood. Pathology end points, including tissue immunophenotyping, are described after 13 and 26 weeks of treatment and after a 34-week treatment-free (recovery) period. Belimumab was safe and well tolerated in repeat-dose toxicology studies at 5-50 mg/kg for up to 26 weeks. Monkeys exposed to belimumab had significant decreases in peripheral blood B lymphocytes by 13 weeks of exposure, continuing into the recovery period, despite total lymphocyte counts similar to the controls. There were concomitant decreases in spleen and lymph node B-lymphocyte representation after 13 or 26 weeks of treatment with belimumab. Microscopically, monkeys treated with belimumab for 13 or 26 weeks had decreases in the number and size of lymphoid follicles in the white pulp of the spleen. All findings were generally reversible within a 34-week recovery period. These data confirm the specific pharmacologic activity of belimumab in reducing B lymphocytes in the cynomolgus monkey. The favorable safety profile and lack of treatment-related infections also support continued clinical development of belimumab.
A Multi-Task Framework for Weather Recognition (2017)

Weather recognition is important in practice, while this task has not been thoroughly explored so far. The current trend of dealing with this task is treating it as a single classification problem, i.e., determining whether a given image belongs to a certain weather category or not. However, weather recognition differs significantly from traditional image classification, since several weather features may appear simultaneously. In this case, a simple classification result is insufficient to describe the weather condition. To address this issue, we propose to provide auxiliary weather related information for comprehensive weather description. Specifically, semantic segmentation of weather-cues, such as blue sky and white clouds, is exploited as an auxiliary task in this paper. Moreover, a convolutional neural network (CNN) based multi-task framework is developed which aims to concurrently tackle weather category classification task and weather-cues segmentation task. Due to the intrinsic relationships between these two tasks, exploring auxiliary semantic segmentation of weather-cues can also help to learn discriminative features for the classification task, and thus obtain superior accuracy. To verify the effectiveness of the proposed approach, extra segmentation masks of weather-cues are generated manually on an existing weather image dataset. Experimental results have demonstrated the superior performance of our approach. The enhanced dataset, source codes and pre-trained models are available at https://github.com/wzgwzg/Multitask_Weather.
Successful Treatment of Vulvar Intraepithelial Neoplasia with Topical Imiquimod 5% Cream in a Lung Transplanted Patient (2002)

Sir, Imiquimod, an immune response modi® er, has been successful in the treatment of external genital and perianal warts caused by low-risk HPV, usually types 6 or 11 (1). The drug’s antiviral and antitumour properties are thought to be due to its induction of cytokines, which stimulate a T-helper 1 or cell-mediated immune response. Recently, it has been shown that imiquimod may potentially be effective in treating genital intraepithelial neoplasia caused by high-grade HPV (2, 3). We report the case of a 27-year-old woman who presented with recurrent genital warts and later found to have cervical intraepithelial neoplasia (CIN) 2 and vulvar intraepithelial neoplasia (VIN) 3. Two separate courses of topical imiquimod cream 5% were used with clearing of the lesions. Topical imiquimod cream 5% may therefore be an effective medical treatment for genital intrapepithelial neoplasia.
Micelles as Intermediates in the Preparation of Protein−Liposome Conjugates (2006)

A method for preparing protein-liposome conjugates based on micelles as intermediates was developed. Ovalbumin was thiolated with 2-IT and conjugated to the surface of micelles composed of a maleimide-derivatized active lipid and a micelle-forming lipid. These micelles were then incubated with liposomes, allowing the micelle components to exchange into the liposome bilayers. Using this technique we were able to demonstrate that it was possible to saturate the surface of the micelle with protein and use this property to control the level of conjugation. Titration of these protein-micelle conjugates into liposome solutions resulted in reproducible batches of protein-liposome conjugates. Chemical cross-linking could be observed in some cases; however, this was controllable through selection of reagent concentrations. The effects of parameters such as thiolation levels, micelle lipid composition, active lipid structure, micelle-forming lipid structure, and micelle/liposome/protein ratios were examined. The method represents a general approach to the preparation of well defined and reproducible protein-liposome-based drug formulations.
LUCIFER, A CRYPTOGRAPHIC ALGORITHM (1984)

Lucifer, a direct predecessor of the DES algorithm, is a block-cipher having a 128 bit block size and 128 bit key length. Its general design principles and properties are described and discussed. A simple FORTRAN program is presented which implements the algorithm, providing a modern, secure cryptographic algorithm that can be used in personal computers. Lucifer is of special interest because it is in the same class of product ciphers as DES but is much simpler. Study of Lucifer may reveal cryptanalytic methods that can be applied to DES.
Gene expression of the cardiac Na(+)-Ca2+ exchanger in end-stage human heart failure. (1994)

The regulation of cytosolic Ca2+ concentration during excitation-contraction coupling is altered in the failing human heart. Previous studies have focused on disturbances in Ca2+ release and reuptake from the sarcoplasmic reticulum (SR), whereas functional studies of the cardiac Na(+)-Ca2+ exchanger, another important determinant of myocyte homeostasis, are lacking for the failing human heart. Using a cardiac Na(+)-Ca2+ exchanger cDNA recently cloned from a guinea pig cDNA library, we investigated the gene expression of the cardiac Na(+)-Ca2+ exchanger in relation to the SR Ca(2+)-ATPase. Expression of both genes was quantified in left ventricular myocardium from 24 failing human cardiac explants and 7 control heart samples in relation to beta-myosin heavy chain mRNA by slot blot analysis. Compared with patients with nonfailing hearts, patients with dilated cardiomyopathy (DCM, n = 13) showed a 55% increase in Na(+)-Ca2+ exchanger mRNA levels (P < .05 versus control value) and a 41% increase in patients with coronary artery disease (CAD, n = 11). In the same hearts, SR Ca(2+)-ATPase mRNA levels were decreased by 50% in DCM and by 45% in CAD (P < .05 for both versus control value). There was a positive correlation between Na(+)-Ca2+ exchanger and SR Ca(2+)-ATPase mRNA levels both in normal and failing human hearts, albeit with different slopes and intercepts of the regression line. The Na(+)-Ca2+ exchanger protein levels as assessed by Western blot analysis and normalized to beta-myosin heavy chain protein were increased in DCM and CAD (P < .05 and P < .01 versus control value, respectively), whereas SR Ca(2+)-ATPase protein levels were reduced (P < .05 for both groups versus control values). Thus, the Na(+)-Ca2+ exchanger gene expression is enhanced in failing human hearts and may, in part, compensate for the depressed SR function with regard to diastolic Ca2+ removal.
Organization of four mouse lambda light chain immunoglobulin genes. (1981)

We have cloned four lambda light chain constant region (C) genes from mouse embryo DNA. Each carries its own joining (J) segment approximately 1.3 kilobases to its 5' side. The four C genes occur in two clusters, 5' J3C3J1C13' and 5' J2C2J4C43', with C4 being a new C lambda gene. We have also shown that V lambda 1 is joined productively with C lambda 3 in a lambda 3-producing myeloma, and it is most likely that V lambda 1 and V lambda 2 are the only V lambda genes. Based on the analysis of the germ line and rearranged variable region (V) lambda genes in myelomas we argue that the V lambda 1 and V lambda 2 genes are at the 5' side of the C3C1 and C2C4 clusters, respectively. We propose that the two clusters arose by duplication. We also speculate on the role of J-associated DNA sequences in regulation of expression of the lambda subtypes.
Regulatory mechanisms underlying C4 photosynthesis (2011)

C4 photosynthesis is an adaptation that evolved to alleviate the detrimental effects of photorespiration as a result of the gradual decline in atmospheric carbon dioxide levels. In most C4 plants, two cell types, bundle sheath and mesophyll, cooperate in carbon fixation, and, in so doing, are able to alleviate photorespiratory losses. Although much of the biochemistry is well characterized, little is known about the genetic mechanisms underlying the cell-type specificity driving C4 . However, several studies have shown that regulation acts at multiple levels, including transcriptional, post-transcriptional, post-translational and epigenetic. One example of such a regulatory mechanism is the cell-specific accumulation of major photorespiratory transcripts/proteins in bundle sheath cells, where ribulose-1,5-bisphosphate carboxylase/oxygenase is localized. Although many of the genes are expressed in the bundle sheath, some are expressed in both cell types, implicating post-transcriptional control mechanisms. Recently, ultra-high-throughput sequencing techniques and sophisticated mass spectrometry instrumentation have provided new opportunities to further our understanding of C4 regulation. Computational pipelines are being developed to accommodate the mass of data associated with these techniques. Finally, we discuss a readily transformable C4 grass--Setaria viridis--that has great potential to serve as a model for the genetic dissection of C4 photosynthesis in the grasses.
The Pfam protein families database (2009)

Pfam is a widely used database of protein families and domains. This article describes a set of major updates that we have implemented in the latest release (version 24.0). The most important change is that we now use HMMER3, the latest version of the popular profile hidden Markov model package. This software is approximately 100 times faster than HMMER2 and is more sensitive due to the routine use of the forward algorithm. The move to HMMER3 has necessitated numerous changes to Pfam that are described in detail. Pfam release 24.0 contains 11,912 families, of which a large number have been significantly updated during the past two years. Pfam is available via servers in the UK (http://pfam.sanger.ac.uk/), the USA (http://pfam.janelia.org/) and Sweden (http://pfam.sbc.su.se/).
Bacteria-triggered CD4+ T Regulatory Cells Suppress Helicobacter hepaticus–induced Colitis (2002)

We have previously demonstrated that interleukin (IL)-10-deficient (IL-10 knockout [KO]) but not wild-type (WT) mice develop colitis after infection with Helicobacter hepaticus. Here, we show that infected recombination activating gene (RAG) KO mice develop intestinal inflammation after reconstitution with CD4(+) T cells from IL-10 KO animals and that the cotransfer of CD4(+) T cells from H. hepaticus-infected but not uninfected WT mice prevents this colitis. The disease-protective WT CD4(+) cells are contained within the CD45RB(low) fraction and unexpectedly were found in both the CD25(+) and the CD25(-) subpopulations of these cells, their frequency being higher in the latter. The mechanism by which CD25(+) and CD25(-) CD45RB(low) CD4(+) cells block colitis involves IL-10 and not transforming growth factor (TGF)-beta, as treatment with anti-IL-10R but not anti-TGF-beta monoclonal antibody abrogated their protective effect. In vitro, CD45RB(low) CD4(+) cells from infected WT mice were shown to produce IL-10 and suppress interferon-gamma production by IL-10 KO CD4(+) cells in an H. hepaticus antigen-specific manner. Together, our data support the concept that H. hepaticus infection results in the induction in WT mice of regulatory T cells that prevent bacteria-induced colitis. The induction of such cells in response to gut flora may be a mechanism protecting normal individuals against inflammatory bowel disease.
Protection of bone marrow transplant recipients from lethal doses of methotrexate by the generation of methotrexate-resistant bone marrow. (1987)

To develop a highly efficient means for generating methotrexate resistant (MTXr) hematopoietic cells in vivo, a recombinant retroviral genome was constructed that encodes a MTXr dihydrofolate reductase (DHFRr). Cell lines producing high titers of virus capable of transmitting the DHFR gene were generated and used to infect mammalian cells in vitro. Analysis of infected fibroblasts indicated that the DHFRr gene was transmitted intact and conferred a high level of MTXr upon cells. Based on these findings, DHFRr-containing virus was used to infect murine bone marrow cells in vitro. Following infection, the transduced cells were introduced into lethally irradiated recipients via bone marrow transplantation techniques. The presence of the proviral sequences in cells of the spleen and bone marrow of engrafted recipients was associated with significantly increased survival of mice treated with otherwise lethal doses of MTX.
El agua como elemento multidimensional (2004)

El Manifiesto del Agua Autor: Ricardo Petrella Icaria, Barcelona 2002. El caracter critico de la obra de Petrella en los diferentes aspectos que intervienen en la crisis del agua es interesante para ver las deficiencias del sistema, pero deja insatisfecho a aquellos lectores que buscan propuestas para solucionar la crisis.
Binding of insulin-like growth factor II (IGF-II) by human cation-independent mannose 6-phosphate receptor/IGF-II receptor expressed in receptor-deficient mouse L cells. (1990)

Mouse L cells deficient in expression of the murine cation-independent mannose 6-phosphate receptor/insulin-like growth factor II receptor (CI-MPR/IGF-IIR) were stably transfected with a plasmid containing the cDNA for the human receptor. Transfected cells expressed high levels of the human receptor which functioned in the transport of lysosomal enzymes and was capable of binding 125I-IGF-II, both at the cell surface and intracellularly. Cell surface binding of 125I-IGF-II by the receptor could be inhibited by pretreatment of cells with antibodies to the receptor or by coincubation with the lysosomal enzyme, beta-glucuronidase. Expression of the receptor conferred on transfected cells the ability to internalize and degrade 125I-IGF-II. Cells transfected with the parental vector and those expressing the human CI-MRP/IGF-IIR were found to express an atypical binding site for IGF-II that was distinct from the CI-MPR/IGF-IIR and the type I IGF-receptor. The availability of two cell lines, one of which overexpresses the human CI-MPR/IGF-IIR and one deficient in expression of the murine receptor, may help in the analysis of the role of the receptor in mediating the biological effects of IGF-II. They should also be useful in examining the significance of binding of ligands, such as transforming growth factor-beta 1 precursor and proliferin to this receptor.
Wolff-Parkinson-White syndrome with an unroofed coronary sinus without persistent left superior vena cava treated with catheter cryoablation. (2008)

Coronary sinus anomalies are rare congenital defects which are usually coexistent with a persistent left superior vena cava and may be associated with cardiac arrhythmias. We report an unroofed coronary sinus without persistent left superior vena cava diagnosed during a catheter ablation procedure for Wolff-Parkinson-White syndrome. Diagnostic and therapeutic options and outcomes are discussed. This condition is of relevance to electrophysiologists performing catheter-based procedures, as well as cardiologists implanting coronary sinus pacing leads, who may encounter this anomaly in their practice.
Clinical Significance and Molecular Characteristics of Circulating Tumor Cells and Circulating Tumor Microemboli in Patients With Small-Cell Lung Cancer (2012)

Both baseline CTC number and change in CTC number after one cycle of chemotherapy are independent prognostic factors for SCLC. Molecular comparison of CTCs to cells in CTM may provide novel insights into SCLC biology.
Grating Couplers for Fiber-to-Fiber Characterizations of Stand-Alone Dielectric Loaded Surface Plasmon Waveguide Components (2012)

Dielectric loaded gratings (DLGs) comprised of polymer gratings lying on a thin gold film are used to couple light at telecommunication frequencies in and out of plasmonic waveguides featuring sub-micron cross-sections. The grating couplers are found to be efficient and easy to implement to perform direct fiber-to-fiber telecommunication characterizations of dielectric loaded surface plasmon polariton waveguide (DLSPPW) components. By analyzing the dispersion of the plasmonic Bloch modes supported by DLGs as a function of the period and the filling factor of the gratings, efficient couplers comprised of gratings with a filling factor around 0.5 are designed and fabricated by a simple one-step electron beam lithography process. Typical losses in the range of <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">${-}10$</tex></formula> dB per coupler are obtained for gratings designed to operate at normal and 30 <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$^{\circ}$</tex></formula> -tilted incidence. The performance of the couplers for normal incidence can be further improved by adding a back-reflecting Bragg mirror. We demonstrate the transmission of a 10 Gbits/s signal along a 75 <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\mu$</tex> </formula> m-long DLSPPW by using DLG couplers for light injection and extraction. A power penalty below below 0.4 dB on the bit-error-rate has been measured over the entire C-band demonstrating the suitability of DLSPPWs for Wavelength-Division-Multiplexed high bit rate traffic and the efficiency of DLG couplers for fiber-to-fiber characterizations of stand alone DLSPPW components.
Limit of detection (LQD)/limit of quantitation (LOQ): comparison of the empirical and the statistical methods exemplified with GC-MS assays of abused drugs (1994)

The limit of detection (LOD) for any analytical procedure, the point at which analysis is just feasible, may be determined by a statistical approach based on measuring replicate blank (negative) samples or by an empirical approach, consisting of measuring progressively more dilute concentrations of analyte. The limit of quantitation (LOQ), or concentration at which quantitative results can be reported with a high degree of confidence, may likewise be determined by either approach. We used both methods to determine LOD and LOQ for forensic gas chromatographic-mass spectrometric (GC-MS) analyses of abused drugs. The statistically determined LOD and LOQ values for these assays underestimated the LOD because of the large imprecision associated with blank measurements and the inability of blank samples to meet typical GC-MS acceptance criteria. The empirical method provided much more realistic LOD values, supported by reasonable experimental data, and are 0.5-0.03 the magnitude of the corresponding statistical LODs. The empirical LODs and LOQs are identical for these GC-MS assays. The observations made here about the LOD/LOQ for specific forensic GC-MS procedures are generally applicable to any type of analysis.
Paper‐Based ELISA (2010)

Nicht von Pappe: Ein papierbasierter indirekter ELISA-Test wurde entwickelt (siehe Bild) und zum Nachweis von Kaninchen-IgG und dem gp41-Hüllprotein von HIV-1 genutzt. Das Verfahren kombiniert die Empfindlichkeit und Spezifität des ELISA-Tests mit den geringen Kosten und der einfachen Anwendung papierbasierter Analysesysteme. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Immunogenicity of peptides bound to MHC class I molecules depends on the MHC-peptide complex stability (1996)

The impact of the MHC class I peptide binding stability on the immunogenicity of particular peptide Ags in class I-restricted cytotoxic T lymphocyte responses is not clearly established. Therefore, we have determined the dissociation rate of each peptide from MHC class I at 37 degrees C and compared this to that of a consensus CTL epitope. Newly defined immunogenic peptides formed relatively stable MHC-peptide complexes as shown by their low dissociation rates, whereas nonimmunogenic peptides displayed high dissociation rates. In addition virtually all previously described HLA-A*0201-restricted T cell epitopes showed low dissociation rates. Furthermore, we show that the immunogenicity of HIV-1-derived peptides can be predicted more accurately by their dissociation rate than by the MHC class I binding affinity. Selection of peptides based on affinity and their dissociation rate leads to a more precise identification of candidate CTL epitopes than selection based on affinity alone. These results help to understand why some peptides are recognized by CTL and, along with detailed knowledge of protein processing rules, therefore have important implications for the selection of peptides in peptide-based vaccines.