gebhart/patrepeval huggingface dataset:
Structure and reactivity of α,β‐unsaturated ethers. III. Cationic copolymerizations of alkenyl alkyl ethers (1968)

Abstract The relative cationic polymerizabilities of the geometrical isomers of various alkenyl alkyl ethers were studied both in copolymerizations with each other and in their respective copolymerizations with vinyl isobutyl ether as standard. Copolymerizations were carried out in methylene dichloride at −78°C. with boron trifluoride etherate as catalyst. The cis isomers have been found to be more reactive than the corresponding trans isomers. A primary alkyl substituent on the β‐ cis position of vinyl ethyl ether enhances the reactivity. Yet the steric effect is noticeable when the substituents are bulky. Compounds substituted with cis ‐β‐isobutyl and with β‐dimethyl showed little tendency to homopolymerization. It was proved that the polymer ends derived from cis and from trans monomers are respectively different in character because of the restricted rotation of the end unit around the terminal carbon–carbon bond. The alternation tendency, remarkable in the copolymerization of cis monomers with vinyl ether, was explained in terms of the cis ‐opening mechanism.
Development of preferred orientation in polycrystalline TiN layers grown by ultrahigh vacuum reactive magnetron sputtering (1995)

The preferred orientation of polycrystalline TiN films grown by ultrahigh-vacuum reactive-magnetron sputter deposition on amorphous SiO2 at 350 °C in pure N2 discharges was controllably varied from (111) to completely (002) by varying the incident ion/metal flux ratio Ji/JTi from 1 to ≥5 with the N+2 ion energy Ei maintained constant at ≂20 eV (≂10 eV per incident accelerated N). All samples were slightly over-stoichiometric with N/Ti=1.02±0.03. Films deposited with Ji/JTi=1 initially exhibit a mixed texture with competitive columnar growth which slowly evolves into a nearly complete (111) texture at film thicknesses greater than 1 μm. However, films grown with Ji/JTi≥5 exhibit an essentially complete (002) preferred orientation from the earliest observable stages. The normalized XRD (002) intensity ratio in thick layers increased from ≂0 to 1 as Ji/JTi was varied from 1 to ≥5. Both (111) and (001) interplanar spacings remained constant as a function of film thickness yielding a lattice constant of 0.4240±0.0005 nm, equal to that of unstrained bulk TiN. Contrary to previous models, the present results establish that TiN preferred orientation can be controlled without introducing large in-plane compressive stress and/or changes in the strain energy as a function of layer thickness.
Expression and Regulation of a Disintegrin and Metalloproteinase (ADAM) 8 in Experimental Asthma (2004)

Asthma, a complex chronic inflammatory pulmonary disorder, is on the rise despite intense ongoing research. To elucidate novel pathways involved in asthma pathogenesis, we used transcript expression profiling in a murine model of asthma. Employing asthma models induced by different allergens (ovalbumin and Aspergillus fumigatus) we uncovered the involvement of ADAM8, a member of a disintegrin and metalloproteinase (ADAM) family. In situ hybridization of mouse lungs revealed strong ADAM8 induction in peribronchial and perivascular inflammatory cells as well as in bronchiolar epithelial cells following allergen challenge. Sequence analysis of lung ADAM8 cDNA identified a novel splice variant of ADAM8 that contained an additional exon in juxtaposition to the transmembrane domain. Allergen-induced ADAM8 mRNA accumulation in the lung was dose- and time-dependent. Transgenic or pharmacologic delivery of interleukin (IL)-4 or IL-13 to the lungs resulted in a marked increase of ADAM8 expression. Gene-targeted mice studies revealed that ovalbumin-induced ADAM8 was largely dependent upon signal transducer and activator of transcription (STAT) 6 and the IL-4 receptor alpha-chain. Thus, ADAM8 is an allergen-, IL-4-, and IL-13-induced gene in the experimental asthmatic lung. Taken together with the role of ADAM33 in asthma, these results suggest that allergic lung responses involve the interplay of diverse members of the ADAM family.
Synthesis and polymerization of 2‐butyl‐7‐methylene‐1,4,6‐trioxaspiro(4,4)nonane (1988)

Abstract 2‐Butyl‐7‐methylene‐1,4,6‐trioxaspiro(4,4)nonane ( 7 ) was prepared by the reaction of 2‐(bromomethyl)‐5‐oxo‐tetrahydrofuran with 1,2‐epoxyhexane, followed by dehydrobromination. Compound 7 could be polymerized by free radical initiators to give a viscous polymer. The IR and NMR spectra of the polymers indicated that the polymer structure contained ester and ketone units in the backbone, and a cyclic acetal side chain. Compound 7 readily copolymerized with acrylonitrile in the presence and absence of radical initiators, but did not copolymerize well with styrene. Ultraviolet spectra suggest that the spontaneous polymerization proceeds via a chargetransfer complex between 7 as an electron donor and AN as an electron acceptor.
Parametric modeling at resist–substrate interfaces (1994)

In the energy range of interest for x-ray lithography, absorption of x rays by atoms results in the emission of photoelectrons and Auger secondary electrons, as well as a shower of low energy electrons. The development of accurate image formation models in x-ray lithography requires that these mechanisms be included. The photoelectron processes produce a redistribution of the x-ray energy over a finite volume. The interaction of the electrons with the medium is complex and can be treated in the framework of a response theory (dielectric function). A simulation code, based on the Monte Carlo method (lesis), has been developed to study the photon energy redistribution in resists, in the vicinity of resist–substrate interfaces and overlayers. The low computational efficiency of the Monte Carlo method motivates the need to parametrize the energy redistribution results of the simulation. This parametrization was performed for a polymethyl-methacrylate/Si interface, as a function of the photon energy. The parametrization can be interpreted as the weighted sum of individual electron responses. The effects on the resist of photoelectrons generated in the substrate are discussed. These effects have been incorporated in an effective dose model that can be used to compute complex interface geometries. The results are particularly relevant for the nanolithography domain (&amp;lt; 100 nm).
Fast 3D Computational Integral Imaging Using Graphics Processing Unit (2012)

In computational integral imaging (II), the elemental images are processed on serial processors to reconstruct one plane (slice) of the 3D scene. In this paper, we present a fast three-dimensional (3D) integral imaging system via a graphics processing unit (GPU) which allows parallel processing with multiple processors. We show that it can significantly accelerate 3D scene reconstruction in II using the GPU based stream-processing model. The streaming version of the ray back propagation algorithm with lookup table is presented. It is demonstrated that the ray back propagation algorithm with a lookup table for the 3D scene reconstruction in II to be processed on parallel processors may greatly improve computational speed while requiring minimally larger memory space as compared with CPU sequential computing. Experimental results verify the feasibility for parallel implementation of 3D integral imaging. To the best of our knowledge, this is the first study on achieving a 3D computational integral imaging system using GPU computing with high parallelism.
Notch1 engagement by Delta-like-1 promotes differentiation of B lymphocytes to antibody-secreting cells (2007)

Notch signaling regulates B and T lymphocyte development and T cell effector class decision. In this work, we tested whether Notch activity affects mature B cell activation and differentiation to antibody-secreting cells (ASC). We show increased frequency of ASC in cultures of splenic B cells activated with LPS or anti-CD40 when provided exogenous Notch ligand Delta-like-1 (Dll1). Our results indicate that Notch–Dll1 interaction releases a default pathway that otherwise inhibits Ig secretion upon B cell activation. Thus, Dll1 enhanced spontaneous Ig secretion by naturally activated marginal zone B and B1 cells and reversed the inhibition of ASC differentiation mediated by B cell receptor crosslinking during LPS. Moreover, suppression of Notch signaling in B cell expression of either a dominant-negative mutant form of Mastermind-like 1 or a null mutation of Notch1 not only prevented Dll1-mediated enhancement of ASC differentiation but also reduced dramatically LPS-induced Ig secretion. Finally, we show that Dll1 and Jagged-1 are differentially expressed in discrete areas of the spleen, and that the effect of Notch engagement on Ig secretion is ligand-specific. These results indicate that Notch ligands participate in the definition of the mature B cell microenvironment that influences their terminal differentiation.
Cell‐free synthesis‐based protein microarrays and their applications (2009)

The entire complement of proteins expressed by the genome of an organism under a set of defined conditions represents the proteome. The need for rapid, efficient, and high-throughput technologies to understand the proteome of organisms have resulted in the development of protein microarrays. Traditional, cell-based technologies for the generation of protein arrays involve laborious and protracted procedures, which also pose several other technical problems. These limitations have been suitably overcome by cell-free expression systems that carry out rapid, in situ synthesis of proteins from their corresponding DNA templates. This article provides an overview of commonly used cell-free systems that have been used for the generation of protein microarrays, with special emphasis on the diverse applications of cell-free synthesis-based protein microarrays.
Speaker-Independent Speech Separation With Deep Attractor Network (2018)

Despite the recent success of deep learning for many speech processing tasks, single-microphone, speaker-independent speech separation remains challenging for two main reasons. The first reason is the arbitrary order of the target and masker speakers in the mixture (permutation problem), and the second is the unknown number of speakers in the mixture (output dimension problem). We propose a novel deep learning framework for speech separation that addresses both of these issues. We use a neural network to project the time-frequency representation of the mixture signal into a high-dimensional embedding space. A reference point (attractor) is created in the embedding space to represent each speaker which is defined as the centroid of the speaker in the embedding space. The time-frequency embeddings of each speaker are then forced to cluster around the corresponding attractor point which is used to determine the time-frequency assignment of the speaker. We propose three methods for finding the attractors for each source in the embedding space and compare their advantages and limitations. The objective function for the network is standard signal reconstruction error which enables end-to-end operation during both training and test phases. We evaluated our system using the Wall Street Journal dataset (WSJ0) on two and three speaker mixtures and report comparable or better performance than other state-of-the-art deep learning methods for speech separation.
Azole fungicides affect mammalian steroidogenesis by inhibiting sterol 14 alpha-demethylase and aromatase. (2002)

Azole compounds play a key role as antifungals in agriculture and in human mycoses and as non-steroidal antiestrogens in the treatment of estrogen-responsive breast tumors in postmenopausal women. This broad use of azoles is based on their inhibition of certain pathways of steroidogenesis by high-affinity binding to the enzymes sterol 14-alpha-demethylase and aromatase. Sterol 14-alpha-demethylase is crucial for the production of meiosis-activating sterols, which recently were shown to modulate germ cell development in both sexes of mammals. Aromatase is responsible for the physiologic balance of androgens and estrogens. At high doses, azole fungicides and other azole compounds affect reproductive organs, fertility, and development in several species. These effects may be explained by inhibition of sterol 14-alpha-demethylase and/or aromatase. In fact, several azole compounds were shown to inhibit these enzymes in vitro, and there is also strong evidence for inhibiting activity in vivo. Furthermore, the specificity of the enzyme inhibition of several of these compounds is poor, both with respect to fungal versus nonfungal sterol 14-alpha-demethylases and versus other P450 enzymes including aromatase. To our knowledge, this is the first review on sterol 14-alpha-demethylase and aromatase as common targets of azole compounds and the consequence for steroidogenesis. We conclude that many azole compounds developed as inhibitors of fungal sterol 14-alpha-demethylase are inhibitors also of mammalian sterol 14-alpha-demethylase and mammalian aromatase with unknown potencies. For human health risk assessment, data on comparative potencies of azole fungicides to fungal and human enzymes are needed.
4.1: Distinguished Paper : High Mobility Top‐Gate Zinc Oxide Thin‐Film Transistors (ZnO‐TFTs) for Active‐Matrix Liquid Crystal Displays (2006)

Abstract High‐performance top‐gate ZnO thin‐film transistors (TFTs) for AM‐LCDs have been developed. Sputtered ZnO was used as an active channel and silicon nitride (SiNx) deposited by plasma enhanced chemical vapor deposition (P‐CVD) was used as a gate insulator. Field effect mobility and threshold voltage of the ZnO‐TFT are 50.3 cm 2 /V⋅sec and 1.1 V, respectively. We first demonstrated a 1.46″ diagonal AM‐LCD driven by ZnO‐TFTs.
Visceral Adiposity and the Prevalence of Hypertension in Japanese Americans (2003)

Greater visceral adiposity increases the odds of hypertension in Japanese Americans independent of other adipose depots and fasting plasma insulin.
Toward Gene Therapy for Gaucher Disease (1991)

We are studying the transfer and expression by retroviral vectors of the human glucocerebrosidase (GC) gene into bone marrow cells as a model of gene therapy for genetic diseases of hematopoietic cells. A simple retroviral vector (G2) was developed that contains a normal human GC cDNA under the control of the Moloney murine leukemia virus long-terminal repeat (LTR) enhancer/promoter. Murine bone marrow was transduced with the G2 vector and maintained in long-term bone marrow culture (LTBMC). Expression of the human GC gene in the transduced murine LTBMC cells exceeded the level of endogenous murine GC mRNA. Murine bone marrow cells were also transduced with G2 and transplanted into irradiated syngeneic recipients. High levels of GC gene transfer and expression were seen in day-12 CFU-S foci, and to a lesser extent in the hematopoietic organs 4 months after gene transfer/bone marrow transplant (BMT). Human bone marrow, from a patient with Gaucher disease, was also used in studies of GC gene transduction. Gene transfer into 35-40% of the Gaucher hematopoietic progenitor cells was achieved, following prestimulation of the marrow with recombinant hematopoietic growth factors. Equal rates of gene transfer were obtained using either total marrow mononuclear cells or progenitor cells enriched 100-fold by immunomagnetic bead separation. GC gene transduction corrected the enzymatic deficiency of the Gaucher marrow. Our results demonstrate the potential utility of retroviral vector-mediated gene transfer for gene therapy of Gaucher disease. Current efforts are aimed at achieving more consistent in vivo GC expression in the murine BMT model and demonstrating transduction of pluripotent human hematopoietic stem cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Differential modulation by sulfhydryl redox agents and glutathione of GABA- and glycine-evoked currents in rat retinal ganglion cells (1995)

Some areas of the mammalian CNS, such as the retina, contain not one but two fast inhibitory neurotransmitter systems whose actions are mediated by GABA and glycine. Each inhibitory receptor system is encoded by a separate gene family and has a unique set of agonists and antagonists. Therefore, in rat retinal ganglion cells we were surprised to find that a single agent, extracellular glutathione, was capable of modulating currents activated by either GABAA or glycine receptor stimulation. Both oxidized and reduced glutathione influence inhibitory neurotransmission in a manner similar to that of the sulfhydryl redox agents dithiothreitol (DTT) and 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). Remarkably, the actions of glutathione are diametrically opposed on the GABAA and glycine systems. In whole-cell recordings of single retinal ganglion cells with patch pipettes, reduced glutathione enhances GABA-evoked currents but decreases glycine-evoked currents. These findings suggest that endogenous redox agents, such as glutathione, may constitute a novel modulatory system for the differential regulation of inhibitory neurotransmission in the mammalian retina.
Engineered antibody Fc variants with enhanced effector function (2006)

Antibody-dependent cell-mediated cytotoxicity, a key effector function for the clinical efficacy of monoclonal antibodies, is mediated primarily through a set of closely related Fcgamma receptors with both activating and inhibitory activities. By using computational design algorithms and high-throughput screening, we have engineered a series of Fc variants with optimized Fcgamma receptor affinity and specificity. The designed variants display >2 orders of magnitude enhancement of in vitro effector function, enable efficacy against cells expressing low levels of target antigen, and result in increased cytotoxicity in an in vivo preclinical model. Our engineered Fc regions offer a means for improving the next generation of therapeutic antibodies and have the potential to broaden the diversity of antigens that can be targeted for antibody-based tumor therapy.
Treatment of Wilson's Disease with Triethylene Tetramine Dihydrochloride (1980)

Wilson's desease is an autosomal recessive disorder characterized by progressive cirrhosis or neurological signs. Early detection and prompt treatment can reverse the relentless course of the disease. Treatment with D-penicillamine substantially improves the outlook for such patients unless undesirable side effects preclude its use. We report the use of triethylene tetramine dihydrochloride (Trien), a new non-sulfhydryl-chelating agent, in a girl who was unable to tolerate D-penicillamine in spite of steroid coverage. The drug has been well tolerated without side effcets for approximately 2 1/2 years. Our patient's favorable clinical response would suggest that Trien is a safe alternative agent for the treatment of Wilson's disease "hcn D-penicillamine is not tolerated.
Identification and characterization of an endogenous chemotactic ligand specific for FPRL2 (2004)

Chemotaxis of dendritic cells (DCs) and monocytes is a key step in the initiation of an adequate immune response. Formyl peptide receptor (FPR) and FPR-like receptor (FPRL)1, two G protein-coupled receptors belonging to the FPR family, play an essential role in host defense mechanisms against bacterial infection and in the regulation of inflammatory reactions. FPRL2, the third member of this structural family of chemoattractant receptors, is characterized by its specific expression on monocytes and DCs. Here, we present the isolation from a spleen extract and the functional characterization of F2L, a novel chemoattractant peptide acting specifically through FPRL2. F2L is an acetylated amino-terminal peptide derived from the cleavage of the human heme-binding protein, an intracellular tetrapyrolle-binding protein. The peptide binds and activates FPRL2 in the low nanomolar range, which triggers intracellular calcium release, inhibition of cAMP accumulation, and phosphorylation of extracellular signal-regulated kinase 1/2 mitogen-activated protein kinases through the G(i) class of heterotrimeric G proteins. When tested on monocytes and monocyte-derived DCs, F2L promotes calcium mobilization and chemotaxis. Therefore, F2L appears as a new natural chemoattractant peptide for DCs and monocytes, and the first potent and specific agonist of FPRL2.
Carboxymethyllysine, an Advanced Glycation End-Product, Promotes the Invasion and Migration of Lung Cancer A549 Cells (2017)

Effects of carboxymethyllysine (CML), an advanced glycation end-product (AGE), at 1, 2, 4, 8 or 16 &mu;mol/l upon invasion and migration of A549 cells, non-small cell lung cancer (NSCLC) cells, were investigated. Results showed that CML at used test doses did not affect A549 cell growth. However, CML at 4-16 &mu;mol/l enhanced both invasion and migration, and stimulated the release of reactive oxygen species, interleukin-6 and tumor necrosis factor-alpha in A549 cells. CML at 2-16 &mu;mol/l increased protein expression of AGE receptor, p47<sup>phox</sup>, intercellular adhesion molecule-1, fibronectin, kappa-B (NF- &kappa;B) p65 and p-p38 in A549 cells. CML only at 4-16 μmol/l increased matrix metalloproteinase-2 expression in A549 cells. These findings indicated that CML might benefit NSCLC metastasis through promoting invasion and migration.
Cardiac-specific overexpression of sarcolipin inhibits sarco(endo)plasmic reticulum Ca 2+ ATPase (SERCA2a) activity and impairs cardiac function in mice (2004)

Sarcolipin (SLN) inhibits the cardiac sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA2a) by direct binding and is superinhibitory if it binds through phospholamban (PLN). To determine whether overexpression of SLN in the heart might impair cardiac function, transgenic (TG) mice were generated with cardiac-specific overexpression of NF-SLN (SLN tagged at its N terminus with the FLAG epitope). The level of NF-SLN expression (the NF-SLN/PLN expression ratio) was equivalent to that which induces profound superinhibition when coexpressed with PLN and SERCA2a in HEK-293 cells. In TG hearts, the apparent affinity of SERCA2a for Ca(2+) was decreased compared with non-TG littermate control hearts. Invasive hemodynamic and echocardiographic analyses revealed impaired cardiac contractility and ventricular hypertrophy in TG mice. Basal PLN phosphorylation was reduced. In isolated papillary muscle subjected to isometric tension, peak amplitudes of Ca(2+) transients and peak tensions were reduced, whereas decay times of Ca(2+) transients and relaxation times of tension were increased in TG mice. Isoproterenol largely restored contractility in papillary muscle and stimulated PLN phosphorylation to wild-type levels in intact hearts. No compensatory changes in expression of SERCA2a, PLN, ryanodine receptor, and calsequestrin were observed in TG hearts. Coimmunoprecipitation indicated that overexpressed NF-SLN was bound to both SERCA2a and PLN, forming a ternary complex. These data suggest that NF-SLN overexpression inhibits SERCA2a through stabilization of SERCA2a-PLN interaction in the absence of PLN phosphorylation and through the inhibition of PLN phosphorylation. Inhibition of SERCA2a impairs contractility and calcium cycling, but responsiveness to beta-adrenergic agonists may prevent progression to heart failure.