gebhart/patrepeval huggingface dataset:
Control of the ERK MAP kinase cascade by Ras and Raf. (1996)

Activation of ERKs plays a central part in the control of cell proliferation. In this chapter, we have concentrated on the identification and regulation of the kinases that activate Mek. We have chosen to focus on this area because of the many puzzles associated with it. Key issues for the future are the need to derive methods to determine what contribution each individual activator makes to both the magnitude and duration of Mek activation and understanding the mechanisms of regulation. Furthermore, we have considered the roles of Raf and the other kinases solely as Mek activators; they may well have other substrates including cdc25A (Galaktionov et al, 1995), I kappa B (Li and Sedivy, 1993) and TP53 (Jamal and Ziff, 1995).
The VMP network adapter board (NAB): high-performance network communication for multiprocessors (1988)

High performance computer communication between multiprocessor nodes requires significant improvements over conventional host-to-network adapters. Current host-to-network adapter interfaces impose excessive processing, system bus and interrupt overhead on a multiprocessor host. Current network adapters are either limited in function, wasting key host resources such as the system bus and the processors, or else intelligent but too slow, because of complex transport protocols and because of an inadequate internal memory architecture. Conventional transport protocols are too complex for hardware implementation and too slow without it. In this paper, we describe the design of a network adapter board for the VMP multiprocessor machine that addresses these issues. The adapter uses a host interface that is designed for minimal latency, minimal interrupt processing overhead and minimal system bus and memory access overhead. The network adapter itself has a novel internal memory and processing architecture that implements some of the key performance-critical transport layer functions in hardware. This design is integrated with VMTP, a new transport protocol specifically designed for efficient implementation on an intelligent high-performance network adapter. Although targeted for the VMP system, the design is applicable to other multiprocessors as well as uni-processors.
Tumour-specific triple-regulated oncolytic herpes virus to target glioma (2016)

Oncolytic herpes simplex virus type 1 (oHSV-1) therapy is an emerging treatment modality that selectively destroys cancer. Here we report use of a glioma specific HSV-1 amplicon virus (SU4-124 HSV-1) to selectively target tumour cells. To achieve transcriptional regulation of the SU4-124 HSV-1 virus, the promoter for the essential HSV-1 gene ICP4 was replaced with a tumour specific survivin promoter. Translational regulation was achieved by incorporating 5 copies of microRNA 124 target sequences into the 3'UTR of the ICP4 gene. Additionally, a 5'UTR of rat fibroblast growth factor -2 was added in front of the viral ICP4 gene open reading frame. Our results confirmed enhanced expression of survivin and eIF4E in different glioma cells and increased micro-RNA124 expression in normal human and mouse brain tissue. SU4-124 HSV-1 had an increased ICP4 expression and virus replication in different glioma cells compared to normal neuronal cells. SU4-124 HSV-1 exerted a strong antitumour effect against a panel of glioma cell lines. Intracranial injection of SU4-124 HSV-1 did not reveal any sign of toxicity on day 15 after the injection. Moreover, a significantly enhanced antitumour effect with the intratumourally injected SU4-124 HSV-1 virus was demonstrated in mice bearing human glioma U87 tumours, whereas viral DNA was almost undetectable in normal organs. Our study indicates that incorporation of multiple cancer-specific regulators in an HSV-1 system significantly enhances both cancer specificity and oncolytic activity.
Focus plus context screens (2002)

Focus plus context screens are wall-size, low-resolution displays with an embedded, high-resolution display region. Focus plus context screens allow users to view details of a document up close, while simultaneously seeing peripheral parts of the document in lower resolution. Application areas range from geographic information systems to interactive simulations and games.
Analysis of Slug Test Data from Hydraulically Fractured Coalbed Methane Wells (1991)

ABSTRACT This paper presents new type curves for analyzing slug tests in hydraulically fractured coal seams. The type curves were developed using a finite-conductivity, vertical fracture model and are presented in terms of three parameters – dimensionless wellbore storage coefficient, dimensionless fracture conductivity, and fracture-face skin. With these new curves, we may estimate the hydraulic fracture half-length, the formation permeability, and the fracture conductivity. We also present a procedure for using the new curves and illustrate the procedure with an example.
Precision of Digital Vision Systems (1983)

The precision of geometric features such as the centroid, area, perimeter, and orientation measured by a 2-D digital vision system is analyzed and tested experimentally. The digitizing error of various geometric features can be expressed in terms of the dimensionless perimeter of the object. As a result of this work, the sampling process can be simplified or eliminated. The analysis is also expanded to cover the 3-D digital vision systems.
Towards the Generation of Distributed Test Cases Using Petri Nets. (1999)

. Test case generation is a means to validate the implementation of a system a posteriori with respect to some given requirements imposed on the system. Current methods for the generation of test cases often rely on an interleaving model of the system, which does not fully cover the situation in reactive systems and systems with asynchronous communication. In this paper we present an approach towards the generation of distributed test cases, using Petri nets as the class of models for system, requirements and test case. 1 Motivation Testing is an important part of system design. In most cases, it is used to check the conformance of an implementation to a system specication. For communication protocols, this type of testing is called Protocol Conformance Testing [4]. Conformance testing is based on a set of requirements, the so{called test purposes, each of which has to be checked during the procedure of testing. Test purposes are identied, specied and later on implemented (in form...
LOCAT (low-dose computed tomography for appendicitis trial) comparing clinical outcomes following low- vs standard-dose computed tomography as the first-line imaging test in adolescents and young adults with suspected acute appendicitis: study protocol for a randomized controlled trial (2014)

ClinicalTrials.gov NCT01925014.
Inhibition of apoptosis by survivin predicts shorter survival rates in colorectal cancer. (1998)

Deregulated inhibition of apoptosis (programmed cell death) may facilitate the insurgence of neoplasia, but whether it also influences the outcome of common cancers has remained controversial. In this study, we investigated the expression of a novel inhibitor of apoptosis, survivin, in colorectal cancer and its relationship with tumor cell apoptosis and overall prognosis. By immunohistochemistry, survivin was expressed in 91 of 171 (53.2%) cases of colorectal carcinomas of histological stages 0 to IV. In contrast, normal colon epithelium did not express survivin. Although survivin expression did not correlate with p53 abnormalities (46.5% versus 58.0%; P = 0.18), survivin-positive cases were strongly associated with bcl-2 expression (72.5% versus 27.4%; P < 0.0001) and reduced apoptotic index (0.76% +/- 0.39% versus 1.17% +/- 0.62%; P < 0.0001). Expression of survivin alone in bcl-2-negative (discordant) cases also resulted in reduced apoptotic index (0.82% +/- 0.57% versus 1.16% +/- 0.66%; P = 0.0046). When analyzed for prognostic significance, patients with low apoptotic index (< 0.97%) had worse survival rates than the group with high apoptosis (P < 0.001), and a multivariate Cox proportional hazard model identified reduced apoptosis as an independent predictive factor for overall survival (P < 0.0001). These data demonstrate that apoptosis inhibition by survivin, alone or in cooperation with bcl-2, is an important predictive/prognostic parameter of poor outcome in colorectal carcinoma and identify survivin as a new diagnostic/therapeutic target in cancer.
Apoptosis and the pathogenesis of IDDM: a question of life and death. (1998)

In type 1 diabetes, an immune-mediated process leads to the destruction of pancreatic beta-cells. In the last decade, considerable progress has been made in understanding the cellular and biochemical pathogenic processes of the disease. However, more needs to be learned about the immune mechanisms leading to the development of autoreactive immune cells and the molecular mechanisms of beta-cell death. The study of apoptosis of autoreactive lymphocytes as well as apoptosis of beta-cells may give answers to many still unsolved questions. This review focuses on the possible role of apoptosis both in the regulation of immune mechanisms involved in the pathogenesis of type 1 diabetes and as a way for beta-cells to die. The advancement in the knowledge of the possible role of apoptosis and its regulation in the pathogenesis of type 1 diabetes may provide new therapeutic tools for the prevention of the disease.
Enzyme Kinetics (1976)

Journal Article Enzyme Kinetics Get access Irwin H. Segel Irwin H. Segel Department of Biochemistry and Biophysics, University of California, Davis, CA 95616 Search for other works by this author on: Oxford Academic Google Scholar BioScience, Volume 26, Issue 7, July 1976, Pages 425–426, https://doi.org/10.2307/1297475 Published: 01 July 1976
Lipid profile of Helicobacter spp.: presence of cholesteryl glucoside as a characteristic feature (1996)

The lipid and fatty acid profiles of eight Helicobacter spp. (H. nemestrinae, H. acinonyx, H. canis, Helicobacter sp. strain CLO-3, "H. rappini" [Flexispira rappini], H. pametensis, Helicobacter sp. strain Bird-B, and Helicobacter sp. strain Bird-C) and the fatty acid profiles of five additional species (H. pylori, H. felis, H. muridarum, H. mustelae, and H. fennelliae) were analyzed and compared. A heterologous fatty acid profile was observed among the Helicobacter spp., and on that basis the species could be divided into two groups. Group A had 19-carbon cyclopropane fatty acid (19:0cyc) and tetradecanoic acid (14:0) as the major fatty acids, and group B characteristically lacked the 19:0cyc and had hexadecanoic acid (16:0) and octadecenoic (18:1) acids as the major fatty acids. The species of group A are primarily gastric colonizers, and those of group B are primarily intestinal colonizers. Seven of the eight species studied showed the unusual and characteristic presence of cholesteryl glucosides (CGs), and most of these seven showed a very large amount (9.7 to 27.4% of the weight of total extractable lipid). The types of CGs and their distribution in different species were as follows: cholesteryl-6-O-acyl-alpha-D-glucopyranoside (cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside in H. nemestrinae and mainly cholesteryl-6-O-dodecanoyl-alpha-D-glucopyranoside in "H. rappini"), cholesteryl-alpha-D-glucopyranoside (H. nemestrinae, H. acinonyx, H. canis, Helicobacter sp. strain CLO-3, and "H. rappini"), and cholesteryl-6-O-phosphatidyl-alpha-D-glucopyranoside (H. nemestrinae, H. acinonyx, H. canis, and Helicobacter sp. strain CLO-3). Besides this, we could also detect cholesteryl acyl glucoside in H. acinonyx, cholesteryl glucoside in Helicobacter sp. strains Bird-B and -C, and cholesteryl phosphatidyl glucoside in "H. rappini" and Helicobacter sp. strain Bird-C. A selective accumulation of free cholesterol was observed in the neutral lipid fractions. On the basis of the detection of CGs in 11 of the 13 species studied so far, the presence of CGs appears to be a characteristic feature of the genus Helicobacter. In view of this and also because of a simple and rapid detection method described herein, the CGs can be used as a valuable chemotaxonomic marker.
Determinants of Lighting Quality II: Research and Recommendations. (1996)

Lighting practitioners and laypeople alike believe that the quality of the luminous environment can influence task performance, comfort, and well-being. There is mounting concern that the quality of the lit environment will decline in parallel to lighting energy use as energy codes and standards come into effect; however, there is no consensus about what constitutes good lighting quality. Broad agreement exists that illuminance, luminance, luminance distribution, uniformity, glare control, flicker rate, and spectral power distribution are the important dimensions of the luminous environment. This literature review relates these variables to behavioural outcomes such as visual comfort, task performance, preferences, and well-being. Comparison of these studies to the recommended practices for lighting design in offices reveals little agreement. Psychologists and other behaviour scientists, although not generally accustomed to participating in the writing of codes and standards, have an important role to play the development of these regulations, in ensuring that the best possible knowledge base is applied to the establishment of working and living conditions to support people. Rsum Tout comme les profanes, les praticiens de l&amp;apos;clairage croient que la qualit de l&amp;apos;environnement lumineux peut influer sur l&amp;apos;accomplissement des tches ainsi que sur le confort et le bien-tre des personnes. On s&amp;apos;inquite de plus en plus de la diminution prvue de la qualit du milieu clair la suite de la mise en place de codes et normes rglementant l&amp;apos;utilisation de l&amp;apos;nergie; cependant, il n&amp;apos;y a pas de consensus sur ce qui constitue une bonne qualit d&amp;apos;clairage. On admet gnralement que l&amp;apos;clairement, la luminance, la rpartition de la luminance, l&amp;apos;uniformit, le contrle de l&amp;apos;blouissement, la vitesse de ...
Ovarian granulosa-stromal cell tumors are characterized by trisomy 12. (1991)

Eleven ovarian granulosa-stromal cell tumors including 1 thecoma, 2 fibromas, 6 fibrothecomas, and 2 granulosa cell tumors, were karyotyped after direct harvest and/or short-term tissue culture. Bilateral fibrothecomas from one patient appeared to lack cytogenetic aberrations: the remaining nine tumors were characterized by trisomy for chromosome 12. Cytogenetic aberrations in the two granulosa cell tumors were much less complex than those described previously in undifferentiated carcinomas; accordingly cytogenetic analyses might be useful in distinguishing these categories. The consistent occurrence of trisomy 12 in different varieties of granulosa-stromal cell tumors suggests a common mechanism of oncogenesis within this diverse group of neoplasms. That mechanism probably involves promotion of low-grade, orderly cell proliferation.
A Simple and Reliable Method for Screening Retroviral Producer Clones Without Selectable Markers (1997)

Simplified retroviral vectors that lack dominant selectable markers are being used with increasing frequency. These simplified vectors may offer a number of advantages over selectable marker-containing constructs, including potentially higher titers and less immunogenicity. However, the use of these vectors has been limited by the cumbersome experimental approaches in establishing and characterizing useful producer cell clones. To address this issue, a simple and reliable assay was developed to identify retroviral producer cell lines with or without dominant selectable markers. Producer cells were first generated by standard transfection/transduction and clones isolated by limiting dilution. Supernatant from each clone was then screened by RNA dot blot to identify the best producer clone candidates. The semiquantitative nature of the RNA dot blot assay was validated using a retroviral vector containing neomycin phosphotransferase (neo). Titers obtained by conventional G418-resistant colony forming units/ml (G418(R) cfu/ml) assays strongly correlated with the values by RNA dot blot procedure. RNA dot blot results also correlated well with titers estimated by Southern analysis of HeLa cells transduced with supernatant from each clone. The RNA dot blot technique is a rapid (2 days) and reliable method to screen retroviral producer cells, thereby facilitating the generation and characterization of simplified retroviral producer cell clones.
The Usefulness of Prostate-Specific Antigen and Prostatic Acid Phosphatase in Clinical Practice (1988)

A comparative study was performed on the usefulness of prostate-specific antigen (PSA) and prostatic acid phosphatase (PAP) in control subjects (69). benign prostatic hypertrophy (BPH) patients (150), and patients with prostatic carcinoma (113) in a urology department. We establish, as others, the greater clinical sensitivity of PSA and its effectiveness as a prognostic tool in the evaluation of prostatic cancer therapy and in the early detection of residual tumor following radical prostatectomy. However, patients are admitted to our department with more severe and complicated benign prostatic pathology and urinary dysfunctions, which decreases the specificity of the PSA test to 30% (N = 2.7 ng/ml). A cutoff threshold of 50 ng/ ml becomes necessary to maintain a 90% positive predictive value. The combination of PSA sensitivity (96%) and PAP specificity (95%) enabled a better definition of the high-risk subpopulation among noncancer patients and. in addition, was a help for differential diagnosis, confirmation of advanced stages of prostatic cancer, and selection of low-stage prostatic cancer candidates undergoing radical prostatectomy. Routine serum PSA measurements in the population of patients consulting a urology department will no doubt bring about a new approach to the management of prostate cancer.
ChemInform Abstract: New Antihistaminic N‐Heterocyclic 4‐Piperidinamines. Part 3. Synthesis and Antihistaminic Activity of N‐(4‐Piperidinyl)‐3H‐imidazo[4,5‐b]pyridin‐2‐amines. (1986)

Abstract Über die Verbindungen (V) erhält man Imidazopyridine (VI), die aber nicht die Antihistamin‐Aktivität von Astemizol (VII) erreichen.
Advanced Non-Small-Cell Lung Cancer (2006)

It's been an interesting time for those of us who treat patients with lung cancer. Over the past few years, non-small-cell lung cancer (NSCLC) has been a target for the numerous companies developing agents that inhibit receptors, growth factors, signaling molecules, and genes involved in tumor growth and development. The biologic-targeted approach to treatment is still in its infancy, but it has already given us great expectations, some surprises, some disappointments, and, ultimately, satisfaction that we now have a nonchemotherapeutic option.
Trimethylene bridges as synthetic spacers for the detection of intramolecular interactions (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTrimethylene bridges as synthetic spacers for the detection of intramolecular interactionsNelson J. LeonardCite this: Acc. Chem. Res. 1979, 12, 12, 423–429Publication Date (Print):December 1, 1979Publication History Published online1 May 2002Published inissue 1 December 1979https://pubs.acs.org/doi/10.1021/ar50144a001https://doi.org/10.1021/ar50144a001research-articleACS PublicationsRequest reuse permissionsArticle Views377Altmetric-Citations111LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Linalool prevents oxidative stress activated protein kinases in single UVB-exposed human skin cells (2017)

Ultraviolet-B radiation (285-320 nm) elicits a number of cellular signaling elements. We investigated the preventive effect of linalool, a natural monoterpene, against UVB-induced oxidative imbalance, activation of mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B (NF-κB) signaling in HDFa cells. We observed that linalool treatment (30 μM) prevented acute UVB-irradiation (20 mJ/cm2) mediated loss of activities of antioxidant enzymes in HDFa cells. The comet assay results illustrate that linalool significantly prevents UVB-mediated 8-deoxy guanosine formation (oxidative DNA damage) rather than UVB-induced cyclobutane pyrimidine (CPD) formation. This might be due to its ability to prevent UVB-induced ROS formation and to restore the oxidative imbalance of cells. This has been reflected in UVB-induced overexpression of MAPK and NF-κB signaling. We observed that linalool inhibited UVB-induced phosphorylation of ERK1, JNK and p38 proteins of MAPK family. Linalool inhibited UVB-induced activation of NF-κB/p65 by activating IκBa. We further observed that UVB-induced expression of TNF-α, IL6, IL-10, MMP-2 and MMP-9 was modulated by linalool treatment in HDFa cells. Thus, linalool protects the human skin cells from the oxidative damages of UVB radiation and modulates MAPK and NF-κB signaling in HDFa cells. The present findings substantiate that linalool may act as a photoprotective agent against UVB-induced skin damages.
Encapsulation of Adipose Stromal Vascular Fraction Cells in Alginate Hydrogel Spheroids Using a Direct-Write Three-Dimensional Printing System (2013)

The study of tissue function in vitro has been aided by the development of three-dimensional culture systems that more accurately duplicate the complex cell components of tissues and organs. Bioprinting of cells provides a rapid tissue fabrication technique that can be used to evaluate normal and pathologic conditions in vitro as well as to construct complex three-dimensional tissue structures for implantation in regenerative medicine therapies. Studies were performed using a direct write three-dimensional bioprinting system to fabricate adipose-derived stromal vascular fraction cell spheroids. Human fat-derived stromal vascular fraction cells were mixed in 1.5% (w/v) alginate solutions, and fabrication conditions were varied to produce an array of spheroids. The spheroids were placed in spinner culture, and spheroid integrity and encapsulated cell viability were assessed for 16 days. Results establish the ability to tightly control adipose SVF spheroids in the range of 800-1500 μm. Fabrication conditions were used to control spheroid size, and the results illustrate the ability to construct spheroids of precise size and shape. The adipose SVF cell population remains viable and the spheroid integrity was maintained for 16 days in suspension culture. The direct-write printing of adipose stromal vascular fraction cell containing spheroids provides a rapid fabrication technology to support in vitro microphysiologic system studies.