gebhart/patrepeval huggingface dataset:
Experimental Identification of Nonlinear Systems (1998)

A procedure is presented for using a primary resonance excitation in experimentally identifying the nonlinear parameters of a model approximating the response of a cantilevered beam by a single mode. The model accounts for cubic inertia and stiffness nonlinearities and quadratic damping. The method of multiple scales is used to determine the frequency-response function for the system. Experimental frequency- and amplitude-sweep data are compared with the prediction of the frequency-response function in a least-squares curve-fitting algorithm. The algorithm is improved by making use of experimentally known information about the location of the bifurcation points. The method is validated by using the parameters extracted to predict the force-response curves at other nearby frequencies. We then compare this technique with two other techniques that have been presented in the literature. In addition to the amplitude- and frequency-sweep technique presented, we apply a second frequency-domain technique and a time-domain technique to the second mode of a cantilevered beam. We apply the restoring-force surface method assuming no a priori knowledge of the system and use the shape of the surface to guide us in assuming a form for the equation of motion. This equation is used in applying the frequency-domain techniques: a backbone curve-fitting technique based on the describing-function method and the amplitude- and frequency-sweep technique based on the method of multiple scales. We derive the equation of motion from a Lagrangian and discover that the form assumed based on the restoring-force surface is incorrect. All of the methods are reapplied with the new form for the equation of motion. Differences in the parameter estimates are discussed. We conclude by discussing the limitations encountered for each technique. These include the inability to separate the nonlinear curvature and inertia effects and problems in estimating the coefficients of small terms with the time-domain technique.
erythro-Diols of wax from the uropygial gland of the turkey (1969)

The uropygial (preen) gland secretion of the domestic turkey resembles that of the chicken in consisting mainly of a diester wax. The esterified fatty acids are saturated; they include all members of the n-C(10)-C(20) homologous series, the C(17)-C(19) acids together accounting for 60% of the total. There are four major 2,3-n-alkanediols, C(19)-C(23), all having the erythro configuration as determined by thin-layer chromatography on boric acid-silica gel and by gas-liquid chromatography. The chicken uropygiols, by contrast, contain erythro and threo diols. It is suggested that the chicken possesses two biosynthetic enzyme systems for the diols, the turkey only one
Digital halftoning technique using a blue-noise mask (1992)

A novel digital halftoning technique, by which the halftoning is achieved by a pixelwise comparison of the gray-scale image to an array (halftone screen), the blue-noise mask, is presented. This mask is designed so that the halftone image has blue-noise (high-frequency) characteristics in the frequency domain. The algorithm for the construction of the blue-noise mask and an algorithm for the construction of binary patterns with the same first-order but different second-order statistics are presented. Two psychovisual tests in which human subjects rated halftone patterns and images according to various criteria are also described.
Functional validation of putative tumor suppressor gene C13ORF18 in cervical cancer by Artificial Transcription Factors (2013)

C13ORF18 is frequently hypermethylated in cervical cancer but not in normal cervix and might serve as a biomarker for the early detection of cervical cancer in scrapings. As hypermethylation is often observed for silenced tumor suppressor genes (TSGs), hypermethylated biomarker genes might exhibit tumor suppressive activities upon re-expression. Epigenetic drugs are successfully exploited to reverse TSG silencing, but act genome-wide. Artificial Transcription Factors (ATFs) provide a gene-specific approach for re-expression of silenced genes. Here, we investigated the potential tumor suppressive role of C13ORF18 in cervical cancer by ATF-induced re-expression. Five zinc finger proteins were engineered to bind the C13ORF18 promoter and fused to a strong transcriptional activator. C13ORF18 expression could be induced in cervical cell lines: ranging from >40-fold in positive (C13ORF18-unmethylated) cells to >110-fold in negative (C13ORF18-methylated) cells. Re-activation of C13ORF18 resulted in significant cell growth inhibition and/or induction of apoptosis. Co-treatment of cell lines with ATFs and epigenetic drugs further enhanced the ATF-induced effects. Interestingly, re-activation of C13ORF18 led to partial demethylation of the C13ORF18 promoter and decreased repressive histone methylation. These data demonstrate the potency of ATFs to re-express and potentially demethylate hypermethylated silenced genes. Concluding, we show that C13ORF18 has a TSG function in cervical cancer and may serve as a therapeutic anti-cancer target. As the amount of epimutations in cancer exceeds the number of gene mutations, ATFs provide promising tools to validate hypermethylated marker genes as therapeutic targets.
N‐back working memory paradigm: A meta‐analysis of normative functional neuroimaging studies (2005)

One of the most popular experimental paradigms for functional neuroimaging studies of working memory has been the n-back task, in which subjects are asked to monitor the identity or location of a series of verbal or nonverbal stimuli and to indicate when the currently presented stimulus is the same as the one presented n trials previously. We conducted a quantitative meta-analysis of 668 sets of activation coordinates in Talairach space reported in 24 primary studies of n-back task variants manipulating process (location vs. identity monitoring) and content (verbal or nonverbal) of working memory. We found the following cortical regions were activated robustly (voxelwise false discovery rate = 1%): lateral premotor cortex; dorsal cingulate and medial premotor cortex; dorsolateral and ventrolateral prefrontal cortex; frontal poles; and medial and lateral posterior parietal cortex. Subsidiary meta-analyses based on appropriate subsets of the primary data demonstrated broadly similar activation patterns for identity monitoring of verbal stimuli and both location and identity monitoring of nonverbal stimuli. There was also some evidence for distinct frontoparietal activation patterns in response to different task variants. The functional specializations of each of the major cortical components in the generic large-scale frontoparietal system are discussed. We conclude that quantitative meta-analysis can be a powerful tool for combining results of multiple primary studies reported in Talairach space. Here, it provides evidence both for broadly consistent activation of frontal and parietal cortical regions by various versions of the n-back working memory paradigm, and for process- and content-specific frontoparietal activation by working memory.
Single-Pulse Phase-Contrast Nonlinear Raman Spectroscopy (2002)

High spectral resolution nonlinear vibrational spectroscopy with a single ultrashort pulse is demonstrated on a variety of samples. The spectral data are obtained by shaping the excitation pulse in order to control the relative phase between the weak resonant signal and the strong nonresonant background, in analogy with phase-contrast microscopy techniques. This is unlike the more conventional approach to nonlinear spectroscopy, in which the nonresonant background is reduced to a minimum. By measuring the spectrum of the coherent anti-Stokes Raman signal, it is possible to infer the vibrational energy levels in a band spanning almost an entire octave.
Primary stimulation by dendritic cells induces antiviral proliferative and cytotoxic T cell responses in vitro. (1989)

We used well-gassed hanging drop (20 microliters) cultures with high concentrations of purified T cells from normal BALB/c mice to examine whether dendritic cells (DC) can induce primary antiviral proliferative T cell responses and generate virus-specific CTL. We found that DC exposed to infectious influenza virus in vitro or in vivo in small numbers (0.1-1%) resulted in strong proliferation of responder T cells within 3 d, and this was strongly inhibited by antibodies to class II MHC molecules. In addition, in 5-d cultures, the influenza-treated DC generated CTL specifically able to lyse influenza-infected syngeneic target cells bearing MHC class I antigens. The most potent nucleoprotein (NP) epitope recognized by BALB/c CTL is peptide 147-158 (Arg156-) and influenza-infected DC in vitro stimulated CTL recognizing this peptide, thus mimicking the response in mice primed by intranasal influenza infection. We also induced T cell proliferation and virus-specific CTL in cultures of normal T cells by stimulating with DC pulsed with the natural NP sequence 147-158 or the potent peptide 147-158 (Arg156-). Small numbers of peritoneal exudate cells, after activation with Con A to produce class II MHC expression and after removal of DC with a specific mAb (33DI), did not lead to primary CTL generation but initiated secondary stimulation in vitro. Our results using the hanging drop culture method and DC as APC have implications for studying the T cell repertoire for viral components in humans without the necessity of previous immunization.
A Prospective Study of the Epidemiology and Ecology of Lassa Fever (1987)

In studies of Lassa fever in Sierra Leone, the prevalence of human antibody to Lassa virus ranged from 8% to 52%. Mastomys natalensis, the reservoir of Lassa virus, constituted 50%-60% of the rodents captured in houses but only 10%-20% of those captured in surrounding agriculture and bush areas (chi 2 = 90.2, P less than 10(-6), df = 1), a finding suggesting that houses are the most-important location for transmission of Lassa virus. Viral infection of Mastomys from houses ranged from 0% to 80%. The incidence of seroconversions in susceptible persons ranged from 5% to 22% per year; the ratio of illness to infection ranged from 9% to 26%, and the proportion of febrile illness associated with seroconversion was 5%-14%. Eightfold rises in titer of antibody occurred in 1%-18% of the antibody-positive population, a result suggesting reinfection. We estimate the ratio of fatalities to infection to be 1%-2%, a rate lower than estimates based on hospitalized cases. The high incidence of Lassa fever makes it a major problem in West Africa.
Isolation and Mapping of Small Cauliflower Mosaic Virus DNA Fragments Active as Promoters in Escherichia coli (1981)

Small EcoRI(*) fragments of cauliflower mosiac virus DNA (strain CM4-184), which act as promoters for the tetracycline resistance gene on the promoter probe plasmid pBRH4 in Escherichia coli, have been isolated and mapped on the viral genome. Two regions of the viral genome contain DNA sequences with promoter activity in E. coli. Two independent cloned fragments from one region direct a high level of tetracycline resistance (up to 38 mug of tetracycline per ml). Two independent fragments from the second region of the viral genome also direct tetracycline resistance, but at lower levels. The activity of the two fragments with the strongest promoter activity in E. coli may direct transcription of the viral genome in a clockwise direction. This is consistent with the direction of transcription predicted from sequence analysis of the viral DNA (Franck et al., Cell 21: 285-294, 1980). One of these fragments maps at the start of a large open translational reading frame which is predicted to contain the coding sequence for the viral coat protein. Each promoter-active fragment is located in the 5'-terminal portion of one of the six open reading frames predicted from the DNA sequence.
New Binding Mode to TNF-Alpha Revealed by Ubiquitin-Based Artificial Binding Protein (2012)

A variety of approaches have been employed to generate binding proteins from non-antibody scaffolds. Utilizing a beta-sheet of the human ubiquitin for paratope creation we obtained binding proteins against tumor necrosis factor (TNF)-alpha. The bioactive form of this validated pharmacological target protein is a non-covalently linked homo-trimer. This structural feature leads to the observation of a certain heterogeneity concerning the binding mode of TNF-alpha binding molecules, for instance in terms of monomer/trimer specificity. We analyzed a ubiquitin-based TNF-alpha binder, selected by ribosome display, with a particular focus on its mode of interaction. Using enzyme-linked immunosorbent assays, specific binding to TNF-alpha with nanomolar affinity was observed. In isothermal titration calorimetry we obtained comparable results regarding the affinity and detected an exothermic reaction with one ubiquitin-derived binding molecule binding one TNF-alpha trimer. Using NMR spectroscopy and other analytical methods the 1:3 stoichiometry could be confirmed. Detailed binding analysis showed that the interaction is affected by the detergent Tween-20. Previously, this phenomenon was reported only for one other type of alternative scaffold-derived binding proteins--designed ankyrin repeat proteins--without further investigation. As demonstrated by size exclusion chromatography and NMR spectroscopy, the presence of the detergent increases the association rate significantly. Since the special architecture of TNF-alpha is known to be modulated by detergents, the access to the recognized epitope is indicated to be restricted by conformational transitions within the target protein. Our results suggest that the ubiquitin-derived binding protein targets a new epitope on TNF-alpha, which differs from the epitopes recognized by TNF-alpha neutralizing antibodies.
The functional neuroanatomy of tinnitus (1998)

We used PET to map brain regions responding to changes in tinnitus loudness in four patients who could alter tinnitus loudness by performing voluntary oral facial movements (OFMs). Cerebral blood flow was measured in four patients and six controls at rest, during the OFM, and during stimulation with pure tones. OFM-induced loudness changes affected the auditory cortex contralateral to the ear in which tinnitus was perceived, whereas unilateral cochlear stimulation caused bilateral effects, suggesting a retrocochlear origin for their tinnitus. Patients, compared with controls, showed evidence for more widespread activation by the tones and aberrant links between the limbic and auditory systems. These abnormal patterns provide evidence for cortical plasticity that may account for tinnitus and associated symptoms. Although audiologic symptoms and examinations of these patients were typical, the unusual ability to modulate tinnitus loudness with an OFM suggests some caution may be warranted in generalizing these findings.
Roll‐to‐Roll Compatible Sintering of Inkjet Printed Features by Photonic and Microwave Exposure: From Non‐Conductive Ink to 40% Bulk Silver Conductivity in Less Than 15 Seconds (2012)

A combination of photonic and microwave flash exposure is used to sinter inkjet printed silver nanoparticles. This approach leads to conductive features on polymer substrates in short times that are compatible with roll-to-roll production. The sequential process of sintering the as-printed features revealed a final conductivity of 40% of bulk silver, in less than 15 seconds. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Dermatan Sulfate and LMW Heparin Enhance the Anticoagulant Action of Activated Protein C (1999)

Unfractionated heparin potentiates the anticoagulant action of activated protein C (APC) through several mechanisms, including the recently described enhancement of proteolytic inactivation of factor V. Possible anticoagulant synergism between APC and physiologic glycosaminoglycans, pharmacologic low molecular weight heparins (LMWHs), and other heparin derivatives was studied. Dermatan sulfate showed potent APC-enhancing effect. Commercial LMWHs showed differing abilities to promote APC activity, and the molecular weight of LMWHs correlated with enhancement of APC activity. Degree of sulfation of the glycosaminoglycans influenced APC enhancement. However, because dextran sulfates did not potentiate APC action, the presence of sulfate groups per se on a polysaccharide is not sufficient for APC enhancement. As previously for unfractionated heparin, APC anticoagulant activity was enhanced by glycosaminoglycans when factor V but not factor Va was the substrate. Thus, dermatan sulfate and LMWHs exhibit APC enhancing activity in vitro that could be of physiologic and pharmacologic significance.
NANOTUBE CATALYSTS (2009)

RETURN TO ISSUEPREVNewsNEXTNANOTUBE CATALYSTSCATALYSIS: Nitrogen-doped carbon nanotubes could make fuel cells more affordableBETHANY HALFORDCite this: Chem. Eng. News 2009, 87, 6, 7Publication Date (Print):February 9, 2009Publication History Published online14 November 2010Published inissue 9 February 2009https://pubs.acs.org/doi/10.1021/cen-v087n006.p007ahttps://doi.org/10.1021/cen-v087n006.p007anewsACS PublicationsCopyright © 2009 AMERICAN CHEMICAL SOCIETYArticle Views26Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Carbon nanotubes,Catalysts,Fuel cells,Nitrogen,Platinum Get e-Alerts
Loss of AP-2 Results in Up-regulation ofMCAM/MUC18 and an Increase in Tumor Growth and Metastasis of Human Melanoma Cells (1998)

MCAM/MUC18 is a cell-surface glycoprotein of 113 kDa, originally identified as a melanoma antigen, whose expression is associated with tumor progression and the development of metastatic potential. We have previously shown that enforced expression of MCAM/MUC18 in primary cutaneous melanoma led to increased tumor growth and metastatic potential in nude mice. The mechanism for up-regulation of MCAM/MUC18 during melanoma progression is unknown. Here we show that up-regulation of MCAM/MUC18 expression in highly metastatic cells correlates with loss of expression of the transcription factor AP-2. The MCAM/MUC18 promoter contains four binding sites for AP-2, and electrophoretic mobility shift assay gels demonstrated that the AP-2 protein bound directly to the MCAM/MUC18 promoter. Transfection of AP-2 into highly metastatic A375SM melanoma cells (AP-2-negative and MCAM/MUC18-positive) inhibited MCAM/MUC18 promoter-driven chloramphenicol acetyltransferase reporter gene in a dose-dependent manner. MCAM/MUC18 mRNA and protein expression were down-regulated in AP-2-transfected but not in control cells. In addition, re-expression of AP-2 in A375SM cells inhibited their tumorigenicity and metastatic potential in nude mice. These results indicate that the expression of MCAM/MUC18 is regulated by AP-2 and that enforced AP-2 expression suppresses tumorigenicity and metastatic potential of human melanoma cells, possibly by down-regulating MCAM/MUC18 gene expression. Since AP-2 also regulates other genes that are involved in the progression of human melanoma such as c-KIT, E-cadherin, MMP-2, and p21(WAF-1), we propose that loss of AP-2 is a crucial event in the development of malignant melanoma.
Irregular Polar Coding for Complexity-Constrained Lightwave Systems (2018)

Next-generation fiber-optic communications call for ultra-reliable forward error correction codes that are capable of low-power and low-latency decoding. In this paper, we propose a new class of polar codes, whose polarization units are irregularly pruned to reduce computational complexity and decoding latency without sacrificing error correction performance. We then experimentally demonstrate that the proposed irregular polar codes can outperform state-of-the-art low-density parity-check (LDPC) codes, while decoding complexity and latency can be reduced by at least 30% and 70%, respectively, versus regular polar codes, while also obtaining a marginal performance improvement.
Cocatalysts for Metal-Catalyzed Olefin Polymerization:  Activators, Activation Processes, and Structure−Activity Relationships (2000)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCocatalysts for Metal-Catalyzed Olefin Polymerization: Activators, Activation Processes, and Structure−Activity RelationshipsEugene You-Xian Chen and Tobin J. MarksView Author Information The Dow Chemical Company, Catalysis R&D, Midland, Michigan 48674 Department of Chemistry, Northwestern University, Evanston, Illinois 60208-3113 Cite this: Chem. Rev. 2000, 100, 4, 1391–1434Publication Date (Web):March 28, 2000Publication History Received13 September 1999Published online28 March 2000Published inissue 1 April 2000https://pubs.acs.org/doi/10.1021/cr980462jhttps://doi.org/10.1021/cr980462jresearch-articleACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views15594Altmetric-Citations1804LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alkyls,Anions,Catalysts,Cations,Sandwich compounds Get e-Alerts