gebhart/patrepeval huggingface dataset:
β1 integrin activation on human neutrophils promotes β2 integrin-mediated adhesion to fibronectin (2001)

Although the importance of beta1 integrin-mediated binding to adhesion molecules and extracellular matrix (ECM) molecules is well established for most types of leukocytes, the expression patterns and functional importance of beta1 integrins on neutrophils have remained controversial. Using flow cytometry, we found that human neutrophils express the alpha4, alpha5, alpha9 and beta1 integrin subunits. To examine whether the integrins VLA-4 (alpha4/beta1) and VLA-5 (alpha5/beta1) have a functional role on neutrophils, we studied adhesion to their ligand fibronectin. Treatment of neutrophils with antibody 8A2, which specifically binds and activates beta1 integrins, resulted in increased binding to fibronectin. However, addition of blocking mAb revealed that 8A2-induced adhesion did not depend on beta1 integrins, but on the beta2 integrin CD11b/CD18. Similarly, activation of beta1 integrins by 8A2 resulted in CD11b-dependent binding of neutrophils to fibrinogen. 8A2 treatment increased expression of an activation epitope of CD11b/CD18, which depended on phosphoinositide 3-OH kinase activity and an adequate concentration of intracellular free Ca2+. These data suggest that engagement of beta1 integrins on neutrophils results in a cross-talk signal that leads to activation of the beta2 integrin CD11b/CD18, followed by CD11b-mediated adhesion. As transmigrated neutrophils are surrounded by both beta1 and beta2 ligands in the ECM, this integrin cross-talk could play a role in modifying migration and cellular activation in inflamed tissues.
The PI‐3 kinase/Akt pathway and T cell activation: pleiotropic pathways downstream of PIP3 (2003)

Ligation of the T cell receptor for antigen (TCR) and/or costimulatory receptor CD28 results in rapid activation of phosphoinositide-3 kinase (PI-3 kinase). It remains unclear, however, precisely how this activation occurs and also how the newly generated phospholipid products trigger the various events associated with T cell activation. Here we discuss the current understanding of how PI-3 kinase is activated by the TCR and CD28 and what roles its products play in T cell activation. We also review recent advances in understanding the function of Akt in particular, especially its role in CD28 costimulation. Several functional targets of Akt are discussed in this regard: inducible transcription, cell survival, glucose metabolism, and the cellular translational machinery. These pathways have been associated with TCR/CD28 costimulation, and they have also been implicated as targets of Akt.
Applications of Raman spectroscopy to pharmacy (2004)

Abstract This paper gives a summary of this special issue on ‘Pharmaceutical Applications of Raman Spectroscopy’. It summarizes the papers collected and introduces the possible applications of classical Raman spectroscopy (macro and micro) and surface‐enhanced Raman spectroscopy to the identification of pharmacologically active substances, their qualitative and quantitative analysis, characterization of crystalline forms and structure determination. Copyright © 2004 John Wiley &amp; Sons, Ltd.
Recent advances in the cannabinoids (2004)

AbstractThis review highlights advances made in cannabinoid chemistry over the time period August 2002 – June 2004. Particular emphasis is placed on new ligands for the CB1 and CB2 receptors. CB1 receptor antagonists have been a prominent area of research for many pharmaceutical companies. Sanofi’s CB1 receptor antagonist rimonabant has now generated clinical data demonstrating its efficacy for both obesity and smoking cessation in human subjects. Recent data showing the effects of CB2 agonists for pain in preclinical animal models has been accompanied by the reports from many laboratories disclosing selective CB2 agonists for the treatment of pain and other indications. The further progression of cannabinoid receptor agonists and antagonists into clinical trials will serve to determine the effectiveness of such compounds as therapeutics in the treatment of human diseases and disorders.Keywordsanandamide transporter inhibitorcannabinoidCB1CB2CB1 agonistCB1 antagonistCB2 agonistCB2 antagonistfatty acid amide hydrolase (FAAH)FAAH inhibitor
Primary Fibromyalgia Syndrome – a Variant of Depressive Disorder? (1989)

Primary fibromyalgia syndrome (PFS) is a form of connective tissue rheumatism, characterized by diffuse chronic pain in periarticular tissue, for which no organic cause can be identified. The present study examined the personal and family history, clinical and psychodynamic features of 40 PFS patients, and compared them to a matched control group of patients suffering from rheumatoid arthritis. Depression, either in the past or at present, was seen significantly more often among PFS patients that among controls. Dependence and passivity, idealization of family relationships, obsessive-compulsive personality, maladaptive response to loss, and prepain ergomania were the psychodynamic features characteristic of PFS patients. It is suggested that PFS is a well-defined disorder, in which specific premorbid, familial, and psychodynamic characteristics result in a depressive disorder which takes the form of a physical symptom: pain.
High speed analog-to-digital conversion with silicon photonics (2009)

Sampling rates of high-performance electronic analog-to-digital converters (ADC) are fundamentally limited by the timing jitter of the electronic clock. This limit is overcome in photonic ADC's by taking advantage of the ultra-low timing jitter of femtosecond lasers. We have developed designs and strategies for a photonic ADC that is capable of 40 GSa/s at a resolution of 8 bits. This system requires a femtosecond laser with a repetition rate of 2 GHz and timing jitter less than 20 fs. In addition to a femtosecond laser this system calls for the integration of a number of photonic components including: a broadband modulator, optical filter banks, and photodetectors. Using silicon-on-insulator (SOI) as the platform we have fabricated these individual components. The silicon optical modulator is based on a Mach-Zehnder interferometer architecture and achieves a V<sub>&pi;</sub>L of 2 Vcm. The filter banks comprise 40 second-order microring-resonator filters with a channel spacing of 80 GHz. For the photodetectors we are exploring ion-bombarded silicon waveguide detectors and germanium films epitaxially grown on silicon utilizing a process that minimizes the defect density.
Transforming growth factor beta stimulates collagen-matrix contraction by fibroblasts: implications for wound healing. (1988)

An important event during wound healing is the contraction of newly formed connective tissue (granulation tissue) by fibroblasts. The role of polypeptide growth factors in the process of wound contraction was investigated by analyzing the influence of transforming growth factor beta (TGF-beta), platelet-derived growth factor on the ability of fibroblasts to contract a collagen matrix in an in vitro system. TGF-beta, but not the other growth factors tested, markedly enhanced the ability of BHK-21,3T3-L1, and human foreskin fibroblasts to contract collagen gels. These results suggest that TGF-beta released from platelets and inflammatory cells at sites of tissue injury stimulates fibroblasts to contract the provisional wound matrix and that this effect contributes to the ability of TGF-beta to accelerate wound healing.
T cell homeostatic proliferation elicits effective antitumor autoimmunity (2002)

Development of tumor immunotherapies focuses on inducing autoimmune responses against tumor-associated self-antigens primarily encoded by normal, unmutated genes. We hypothesized that such responses could be elicited by T cell homeostatic proliferation in the periphery, involving expansion of T cells recognizing self-MHC/peptide ligands. Herein, we demonstrate that sublethally irradiated lymphopenic mice transfused with autologous or syngeneic T cells showed tumor growth inhibition when challenged with melanoma or colon carcinoma cells. Importantly, the antitumor response depended on homeostatic expansion of a polyclonal T cell population within lymph nodes. This response was effective even for established tumors, was characterized by CD8+ T cell–mediated tumor-specific cytotoxicity and IFN-γ production, and was associated with long-term memory. The results indicate that concomitant induction of the physiologic processes of homeostatic T cell proliferation and tumor antigen presentation in lymph nodes triggers a beneficial antitumor autoimmune response.
A noninvasive electromagnetic conductivity sensor for biomedical applications (1988)

The authors present the theory and practice of using a simple coil sensor operated at high frequency (1-10 MHz) to measure changes in conductivity in a biological sample. Explicit results are obtained for symmetric configurations that are useful for calibrating the device and making order-of-magnitude estimates of the results of applying the technique to humans to monitor the onset and progress of brain edema. A simple model suggests that the technique may be able to successfully measure the onset of some medical complications using the technology described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Recombinant human respiratory syncytial virus (RSV)monoclonal antibody Fab is effective therapeutically when introduced directlyinto the lungs of RSV-infected mice. (1994)

Previously, recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fabs were generated by antigen selection from random combinatorial libraries displayed at the tip of filamentous phage. Two such Fabs, which exhibited high binding affinity for RSV F glycoprotein (a major protective antigen), were evaluated for therapeutic efficacy in infected mice just before or at the time of peak virus replication in the lungs. Fab 19, which neutralized RSV infectivity with high efficiency in tissue culture, was effective therapeutically when delivered directly into the lungs by intranasal instillation under anesthesia. In contrast, RSV Fab 126, which failed to neutralize virus in cell culture, did not exhibit a therapeutic effect under these conditions. The amount of Fab 19 required to effect a 5000- to 12,000-fold reduction in titer of RSV in the lungs within 24 hr was rather small. In four separate experiments, a single instillation of 12.9-50 micrograms of RSV Fab 19 was sufficient to achieve such a reduction in pulmonary virus in a 25g mouse. The use of Fabs instead of the whole immunoglobulin molecules from which they are derived reduced the protein content of a therapeutic dose. This is important because the protein load that can be delivered effectively into the lungs is limited. The therapeutic effect of a single treatment with Fab 19 was not sustained, so that a rebound in pulmonary virus titer occurred on the 2nd day after treatment. This rebound in pulmonary RSV titer could be prevented by treating infected mice with a single dose of Fab 19 daily for 3 days. These observations suggest that human monoclonal Fabs grown in Escherichia coli may prove useful in the treatment of serious RSV disease as well as diseases caused by other viruses where replication in vivo is limited primarily to the lumenal lining of the respiratory tract.
SQLVM: Performance Isolation in Multi-Tenant Relational Database-as-a-Service. (2013)

A relational Database-as-a-Service provider, such as Microsoft SQL Azure, can share resources of a single database server among multiple tenants. This multi-tenancy enables cost reduction for the cloud service provider which it can pass on as savings to the tenants. However, resource sharing can adversely affect a tenant’s performance due to resource demands of other tenants ’ workloads. Service providers today do not provide any assurances to a tenant in terms of isolating its performance from other co-located tenants. We present SQLVM, an abstraction for performance isolation which is built on a promise of reservation of key database server resources, such as CPU, I/O and memory, for each tenant. The key challenge is in supporting this abstraction within a DBMS without statically allocating resources to tenants, while ensuring low overheads and scaling to large numbers of tenants. Our contributions are in (1) formalizing the above abstraction of SQLVM; (2) designing mechanisms to support the promised resources; and (3) proposing low-overhead techniques to objectively meter resource allocation to establish accountability. We implemented a prototype of SQLVM in Microsoft SQL Azure and our experiments demonstrate that SQLVM results in significantly improved performance isolation from other tenants when compared to the state-of-the-art. 1.
AFFECTIVE CHARACTERIZATION OF MOVIE SCENES BASED ON CONTENT ANALYSIS AND PHYSIOLOGICAL CHANGES (2009)

In this paper, we propose an approach for affective characterization of movie scenes based on the emotions that are actually felt by spectators. Such a representation can be used to characterize the emotional content of video clips in application areas such as affective video indexing and retrieval, and neuromarketing studies. A dataset of 64 different scenes from eight movies was shown to eight participants. While watching these scenes, their physiological responses were recorded. The participants were asked to self-assess their felt emotional arousal and valence for each scene. In addition, content-based audio- and video-based features were extracted from the movie scenes in order to characterize each scene. Degrees of arousal and valence were estimated by a linear combination of features from physiological signals, as well as by a linear combination of content-based features. We showed that a significant correlation exists between valence-arousal provided by the spectator's self-assessments, and affective grades obtained automatically from either physiological responses or from audio-video features. By means of an analysis of variance (ANOVA), the variation of different participants' self assessments and different gender groups self assessments for both valence and arousal were shown to be significant (p-values lower than 0.005). These affective characterization results demonstrate the ability of using multimedia features and physiological responses to predict the expected affect of the user in response to the emotional video content.
A Low-Cost Approach to Automatically Obtain Accurate 3D Models of Woody Crops (2017)

Crop monitoring is an essential practice within the field of precision agriculture since it is based on observing, measuring and properly responding to inter- and intra-field variability. In particular, "on ground crop inspection" potentially allows early detection of certain crop problems or precision treatment to be carried out simultaneously with pest detection. "On ground monitoring" is also of great interest for woody crops. This paper explores the development of a low-cost crop monitoring system that can automatically create accurate 3D models (clouds of coloured points) of woody crop rows. The system consists of a mobile platform that allows the easy acquisition of information in the field at an average speed of 3 km/h. The platform, among others, integrates an RGB-D sensor that provides RGB information as well as an array with the distances to the objects closest to the sensor. The RGB-D information plus the geographical positions of relevant points, such as the starting and the ending points of the row, allow the generation of a 3D reconstruction of a woody crop row in which all the points of the cloud have a geographical location as well as the RGB colour values. The proposed approach for the automatic 3D reconstruction is not limited by the size of the sampled space and includes a method for the removal of the drift that appears in the reconstruction of large crop rows.
Nitel Araştırmalarda Niteliği Artırma (2010)

Nitel arastirma, kuram olusturmayi temel alan bir anlayisla sosyal olgulari, bagli bulunduklari ortam arastirma plani ve anlamayi on plana alan bir yaklasimdir. nsanlarin yasayislari, yasanmis tecrubeleri, davranislari, duygulari, hisleri isyeri, sosyal hareketler, kulturel olaylar ve uluslar ilgili iliskiler nitel arastirmalari konusu olabilir. Bir son derece sosyal bilmeler niteligi arastirmalarin arttigi gorulur. Bu arastirmalarin artmasiyla birlikte nitel arastirmalarda yeter sorular meydana getirme problemi de gundemdeki yerini sunuyor. Bu kamera nitel arastirmalarda kaynak gecerlik ve guvenirlik (guvenilirlik-vali) kavramlari, bu kavramlara alternatif olarak isci kavramlar ve nitel arastirmalarda beceriler icin ne yapmak teorik olarak devam amaclanmistir. Bu icerida, nitel calisma yapan sablonlarin; tekrarli sorular, rastlantisal orneklem secme, bilesen alintilar yapma, baglantilara yogunlasma, analiz formu, arastirmanin sinirlarini ortaya koyma, kendini degerlendirme (donusluluk, eylemden etkilenme), carpik durumlari bulma, negatif ve alternatif sonuclarla karsilastirma, cesitleme (ucgenleme, sac ayagi), es denetleme (uzman incelemesi), karmasik yoldan denetleme (calisma ozeti), uzun sureli calismalar ve katilimci teyidi (donut alma, iletisimsel dogrulama) gibi stratejileri kullanma calismanin niteligini artiracagi vurgulanmaktadir.
Iron Metabolism in the veal calf (1973)

1. The iron requirements of eighteen Ayrshire bull calves reared on fat-supplemented skim milk for 14 weeks from c. 16 d of age have been studied. There was a highly significant relationship between dietary Fe intake (10, 40 and 100 mg/kg dry diet) and blood haemoglobin concentration, packed cell volume and plasma Fe concentration. 2. A microcytic normochromic anaemia developed in the calves given a diet containing 10 mg Fe/kg diet, but not in the other calves. 3. Plasma Fe concentrations increased to &gt; 5 μg Fe/ml in calves receiving 100 mg Fe/kg diet but there were no effects of Fe treatment on plasma Fe-binding capacity, which rose from 4·9 to 8·4 μg Fe/ml during the experiment. Saturation of plasma transferrin was only 3% in the Fe-deficient calves. 4. There were some significant effects of Fe treatment on tissue concentrations of Fe and cytochrome c. 5. Dietary supplementation with 5 mg copper/kg dry diet had little effect on the growth or haematological status of the calves, although there were significant Cu × Fe interactions for mean corpuscular haemoglobin and mean corpuscular volume. There was no evidence of Cu deficiency in the calves, as measured by blood and liver Cu concentrations and by ceruloplasmin and cytochrome oxidase activities.
Lossless compression of digital audio (2001)

Lossless audio compression is likely to play an important part in music distribution over the Internet, DVD audio, digital audio archiving, and mixing. The article is a survey and a classification of the current state-of-the-art lossless audio compression algorithms. This study finds that lossless audio coders have reached a limit in what can be achieved for lossless compression of audio. It also describes a new lossless audio coder called AudioPak, which low algorithmic complexity and performs well or even better than most of the lossless audio coders that have been described in the literature.
GLUE: A Multi-Task Benchmark and Analysis Platform for Natural Language Understanding (2018)

For natural language understanding (NLU) technology to be maximally useful, both practically and as a scientific object of study, it must be general: it must be able to process language in a way that is not exclusively tailored to any one specific task or dataset. In pursuit of this objective, we introduce the General Language Understanding Evaluation benchmark (GLUE), a tool for evaluating and analyzing the performance of models across a diverse range of existing NLU tasks. GLUE is model-agnostic, but it incentivizes sharing knowledge across tasks because certain tasks have very limited training data. We further provide a hand-crafted diagnostic test suite that enables detailed linguistic analysis of NLU models. We evaluate baselines based on current methods for multi-task and transfer learning and find that they do not immediately give substantial improvements over the aggregate performance of training a separate model per task, indicating room for improvement in developing general and robust NLU systems.
Identification of Mycobacterium spp. by Using a Commercial 16S Ribosomal DNA Sequencing Kit and Additional Sequencing Libraries (2002)

Current methods for identification of Mycobacterium spp. rely upon time-consuming phenotypic tests, mycolic acid analysis, and narrow-spectrum nucleic acid probes. Newer approaches include PCR and sequencing technologies. We evaluated the MicroSeq 500 16S ribosomal DNA (rDNA) bacterial sequencing kit (Applied Biosystems, Foster City, Calif.) for its ability to identify Mycobacterium isolates. The kit is based on PCR and sequencing of the first 500 bp of the bacterial rRNA gene. One hundred nineteen mycobacterial isolates (94 clinical isolates and 25 reference strains) were identified using traditional phenotypic methods and the MicroSeq system in conjunction with separate databases. The sequencing system gave 87% (104 of 119) concordant results when compared with traditional phenotypic methods. An independent laboratory using a separate database analyzed the sequences of the 15 discordant samples and confirmed the results. The use of 16S rDNA sequencing technology for identification of Mycobacterium spp. provides more rapid and more accurate characterization than do phenotypic methods. The MicroSeq 500 system simplifies the sequencing process but, in its present form, requires use of additional databases such as the Ribosomal Differentiation of Medical Microorganisms (RIDOM) to precisely identify subtypes of type strains and species not currently in the MicroSeq library.
On the entry of semliki forest virus into BHK-21 cells (1980)

The pathway by which semliki forest virus (SFV), a membrane-containing animal virus, enters BHK-21 cells was studied morphologically and biochemically. After attaching to the cell surface, the majority of viruses was rapidly trapped into coated pits, internalized by endocytosis in coated vesicles, and sequestered into intracellular vacuoles and lysosomes. Direct penetration of viruses through the plasma membrane was never observed. To assess the possible involvement of lysosomes in the release of the genome into the cytoplasm, the effect of five lysosomotropic agents, known to increase the lysosomal pH, was tested. All of these agents inhibited SFV infectivity and one, chloroquine (the agent studied in most detail), inhibited a very early step in the infection but had no effect on binding, endocytosis, or intracellular distribution of SFV. Thus, the inhibitory effect was concluded to be either on penetration of the nucelocapsid into the cytoplasm or on uncoating of the viral RNA. Possible mechanisms for the penetration of the genome into the cytoplasm were studied in vitro, using phospholipids-cholesterol liposomes and isolated SFV. When the pH was 6.0 or lower, efficient fusion of the viral membranes and the liposomal membranes occurred, resulting in the transfer of the nucleocapsid into the liposomes. Infection of cells could also be induced by brief low pH treatment of cells with bound SFV under conditions where the normal infection route was blocked. The results suggest that the penetration of the viral genome into the cytosol takes place intracellularly through fusion between the limiting membrane of intracellular vacuoles and the membrane of viruses contained within them. The low pH required for fusion together with the inhibitory effect of lysosomotropic agents implicate lysosomes, or other intracellular vacuoles with sufficiently low pH, as the main sites of penetration.