gebhart/patrepeval huggingface dataset:
tmtacn, tacn, and Triammine Complexes of (η6-arene)OsII:  Syntheses, Characterizations, and Photosubstitution Reactions (tmtacn = 1,4,7-Trimethyl-1,4,7-triazacyclononane; tacn = 1,4,7-Triazacyclononane) (2001)

A series of (eta 6-arene)OsII complexes containing the saturated nitrogen donor ligands tmtacn, tacn, and NH3 are prepared and characterized. The electrochemical properties and photochemical reactions of these complexes are studied, and the solid-state structures for [(eta 6-p-cymene)Os(tacn)](PF6)2 (1) and [(eta 6-p-cymene)Os(tmtacn)](PF6)2 (2) are determined. Single-crystal X-ray data: 1, orthorhombic, space group Pbca-D2h15 (No. 61), with a = 14.716(3) A, b = 17.844(3) A, c = 18.350(4) A, V = 4819(2) A3, and Z = 8; 2, monoclinic, space group C2-C2(3) (No. 5), with a = 17.322(4) A, b = 10.481(3) A, c = 15.049(4) A, beta = 98.72 degrees, V = 2701(1) A3, and Z = 4.
Investigations on Pre-Ignition in Highly Supercharged SI Engines (2010)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;This paper presents the results of a study on reasons for the occurrence of pre-ignition in highly supercharged spark ignition engines. During the study, the phenomena to be taken into account were foremost structured into a decision tree according to their physical working principles. Using this decision tree all conceivable single mechanisms to be considered as reasons for pre-ignition could be derived. In order to judge each of them with respect to their ability to promote pre-ignition in a test engine, experimental investigations as well as numerical simulations were carried out.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;The interdependence between engine operating conditions and pre-ignition frequency was examined experimentally by varying specific parameters. Additionally, optical measurements using an UV sensitive high-speed camera system were performed to obtain information about the spatial distribution of pre-ignition origins and their progress.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;The numerical simulations included the analysis of the general auto-ignition behavior of air/fuel mixtures depending on the thermodynamic conditions, mixture composition and other factors by taking into account detailed reaction kinetics. Furthermore, 3D CFD simulations were conducted in order to determine the predominant conditions within the combustion chamber.&lt;/div&gt;&lt;div class="htmlview paragraph"&gt;By combining the results of the experimental and numerical investigations, many of the identified mechanisms could be ruled out. Others were classified to be very improbable. The release of lubricant oil droplets from the cylinder liner turned out to be the most probable explanation for the occurrence of pre-ignition. As a main factor influencing the amount of droplets released and hence the pre-ignition frequency, oil dilution caused by spray impingement due to the lateral injector position was identified.&lt;/div&gt;&lt;/div&gt;
A general primer GP5+/GP6(+)-mediated PCR-enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings (1997)

Two cocktails of digoxigenin-labeled human papillomavirus (HPV) type-specific oligonucleotide probes and an enzyme immunoassay (EIA) were used as a basis to developed a group-specific detection method for 14 high-risk (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and 6 low-risk (types 6, 11, 40, 42, 43, and 44) HPVs, following a general primer GP5+/bioGP6(+)-mediated PCR. The sensitivity of this high-risk/low-risk (HR/LR) HPV PCR-EIA ranged from 10 to 200 HPV copies, depending on the HPV type. Comparison of HR/LR HPV PCR-EIA with radioactive Southern blot hybridization using a general probe on the same PCR products derived from 417 cytomorphologically abnormal cervical scrapings resulted in an overall agreement of 96% between the two methods. Complete concordance between group-specific HR/LR detection and individual typing results for both single and multiple infections indicate the strong specificity of this HR/LR HPV PCR-EIA. Multiple infections could be predicted by comparing PCR-EIA optical density values of the cocktail probes with one of the individual oligonucleotide probes. This novel HR/LR PCR-EIA allows accurate and rapid identification of high-risk and low-risk HPV types in cervical scrapings and will facilitate HPV detection in HPV mass-screening programs.
The Effect of Nanosilica on Cement Matrix Permeability in Oil Well to Decrease the Pollution of Receptive Environment (2011)

Nanosilica is the extremely fine silica that has been used for years as cement admixture both in the oil field and in construction and civil engineering industries. Because of its extremely fine nature and high reactivity pozzolanic material, nanosilica has been used to improve slurry impermeability and the mechanical properties of the hardened material. Using nanosilica in slurry composition, improved reological and mechanical properties of cement slurry and set cement. With the adoption of very fine silica particles in cement matrix, porosity and permeability significantly were declined 33.3% and 99% respectively and compressive strength grew from 1486 psi to 3801 psi. Finally the proper amount of nanosilica in slurry composition is presented. The novel slurry formulation with low porosity and permeability and high compressive strength, is suitable in zones where the possibility of gas migration is very high.
The Results of Extirpation of the Anterior Lobe of the Hypophysis and of the Thyroid of Rana pipiens Larvæ (1916)

casters also present valuable data and opinions as to weather and temperature forecasts in their respective districts.The subject of high winds is efficiently treated by Forecaster E. H. Bowie, who indicates the various types of pressure from which they occur.While pressure gradients induce high winds of definite relative force, yet exceptions to the rule are noted.Hurricanes, northers and blizzards receive due consideration.He mentions the intensity of action, caused by twelve different types of lows.Special supplementary treatment of the storm winds of the Atlantic and Gulf coasts is presented by Professor H. C. Frankenfield, and similar data for the North Pacific coast by Forecaster Bealls, for the South Pacific coast by Forecaster Willson, and for the Great Lakes by Professor Cox.
Managing storage space in a flash and disk hybrid storage system (2009)

This paper considers the problem of efficiently managing storage space in a hybrid storage system employing flash and disk drives. The flash and disk drives exhibit different performance characteristics of read and write behavior. We propose a technique for balancing the workload properties across flash and disk drives in such a hybrid storage system. The presented approach automatically and transparently manages migration of data blocks among flash and disk drives based on their access patterns. This paper presents the design and an evaluation of the proposed approach on a Linux testbed through realistic experiments.
Overexpression of Tau Protein Inhibits Kinesin-dependent Trafficking of Vesicles, Mitochondria, and Endoplasmic Reticulum: Implications for Alzheimer's Disease (1998)

The neuronal microtubule-associated protein tau plays an important role in establishing cell polarity by stabilizing axonal microtubules that serve as tracks for motor-protein-driven transport processes. To investigate the role of tau in intracellular transport, we studied the effects of tau expression in stably transfected CHO cells and differentiated neuroblastoma N2a cells. Tau causes a change in cell shape, retards cell growth, and dramatically alters the distribution of various organelles, known to be transported via microtubule-dependent motor proteins. Mitochondria fail to be transported to peripheral cell compartments and cluster in the vicinity of the microtubule-organizing center. The endoplasmic reticulum becomes less dense and no longer extends to the cell periphery. In differentiated N2a cells, the overexpression of tau leads to the disappearance of mitochondria from the neurites. These effects are caused by tau's binding to microtubules and slowing down intracellular transport by preferential impairment of plus-end-directed transport mediated by kinesin-like motor proteins. Since in Alzheimer's disease tau protein is elevated and mislocalized, these observations point to a possible cause for the gradual degeneration of neurons.
The Minimal Active Structure of Human Relaxin-2 (2011)

H2 relaxin is a peptide hormone associated with a number of therapeutically relevant physiological effects, including regulation of collagen metabolism and multiple vascular control pathways. It is currently in phase III clinical trials for the treatment of acute heart failure due to its ability to induce vasodilation and influence renal function. It comprises 53 amino acids and is characterized by two separate polypeptide chains (A-B) that are cross-linked by three disulfide bonds. This size and complex structure represents a considerable challenge for the chemical synthesis of H2 relaxin, a major limiting factor for the exploration of modifications and derivatizations of this peptide, to optimize effect and drug-like characteristics. To address this issue, we describe the solid phase peptide synthesis and structural and functional evaluation of 24 analogues of H2 relaxin with truncations at the termini of its peptide chains. We show that it is possible to significantly truncate both the N and C termini of the B-chain while still retaining potent biological activity. This suggests that these regions are not critical for interactions with the H2 relaxin receptor, RXFP1. In contrast, truncations do reduce the activity of H2 relaxin for the related receptor RXFP2 by improving RXFP1 selectivity. In addition to new mechanistic insights into the function of H2 relaxin, this study identifies a critical active core with 38 amino acids. This minimized core shows similar antifibrotic activity as native H2 relaxin when tested in human BJ3 cells and thus represents an attractive receptor-selective lead for the development of novel relaxin therapeutics.
Granulocyte-Macrophage Colony-Stimulating Factor Prevents Diabetes Development in NOD Mice by Inducing Tolerogenic Dendritic Cells that Sustain the Suppressive Function of CD4+CD25+ Regulatory T Cells (2007)

Autoimmune diabetes results from a breakdown of self-tolerance that leads to T cell-mediated beta-cell destruction. Abnormal maturation and other defects of dendritic cells (DCs) have been associated with the development of diabetes. Evidence is accumulating that self-tolerance can be restored and maintained by semimature DCs induced by GM-CSF. We have investigated whether GM-CSF is a valuable strategy to induce semimature DCs, thereby restoring and sustaining tolerance in NOD mice. We found that treatment of prediabetic NOD mice with GM-CSF provided protection against diabetes. The protection was associated with a marked increase in the number of tolerogenic immature splenic DCs and in the number of Foxp3+CD4+CD25+ regulatory T cells (Tregs). Activated DCs from GM-CSF-protected mice expressed lower levels of MHC class II and CD80/CD86 molecules, produced more IL-10 and were less effective in stimulating diabetogenic CD8+ T cells than DCs of PBS-treated NOD mice. Adoptive transfer experiments showed that splenocytes of GM-CSF-protected mice did not transfer diabetes into NOD.SCID recipients. Depletion of CD11c+ DCs before transfer released diabetogenic T cells from the suppressive effect of CD4+CD25+ Tregs, thereby promoting the development of diabetes. These results indicated that semimature DCs were required for the sustained suppressive function of CD4+CD25+ Tregs that were responsible for maintaining tolerance of diabetogenic T cells in NOD mice.
Framework for instruction-level tracing and analysis of program executions (2006)

Program execution traces provide the most intimate details of a program's dynamic behavior. They can be used for program optimization, failure diagnosis, collecting software metrics like coverage, test prioritization, etc. Two major obstacles to exploiting the full potential of information they provide are: (i) performance overhead while collecting traces, and (ii) significant size of traces even for short execution scenarios. Reducing information output in an execution trace can reduce both performance overhead and the size of traces. However, the applicability of such traces is limited to a particular task. We present a runtime framework with a goal of collecting a complete, machine- and task-independent, user-mode trace of a program's execution that can be re-simulated deterministically with full fidelity down to the instruction level. The framework has reasonable runtime overhead and by using a novel compression scheme, we significantly reduce the size of traces. Our framework enables building a wide variety of tools for understanding program behavior. As examples of the applicability of our framework, we present a program analysis and a data locality profiling tool. Our program analysis tool is a time travel debugger that enables a developer to debug in both forward and backward direction over an execution trace with nearly all information available as in a regular debugging session. Our profiling tool has been used to improve data locality and reduce the dynamic working sets of real world applications.
Entanglement of Atomic Qubits Using an Optical Frequency Comb (2010)

We demonstrate the use of an optical frequency comb to coherently control and entangle atomic qubits. A train of off-resonant ultrafast laser pulses is used to efficiently and coherently transfer population between electronic and vibrational states of trapped atomic ions and implement an entangling quantum logic gate with high fidelity. This technique can be extended to the high field regime where operations can be performed faster than the trap frequency. This general approach can be applied to more complex quantum systems, such as large collections of interacting atoms or molecules.
Networks of gold nanoparticles and bacteriophage as biological sensors and cell-targeting agents (2006)

Biological molecular assemblies are excellent models for the development of nanoengineered systems with desirable biomedical properties. Here we report an approach for fabrication of spontaneous, biologically active molecular networks consisting of bacteriophage (phage) directly assembled with gold (Au) nanoparticles (termed Au-phage). We show that when the phage are engineered so that each phage particle displays a peptide, such networks preserve the cell surface receptor binding and internalization attributes of the displayed peptide. The spontaneous organization of these targeted networks can be manipulated further by incorporation of imidazole (Au-phage-imid), which induces changes in fractal structure and near-infrared optical properties. The networks can be used as labels for enhanced fluorescence and dark-field microscopy, surface-enhanced Raman scattering detection, and near-infrared photon-to-heat conversion. Together, the physical and biological features within these targeted networks offer convenient multifunctional integration within a single entity with potential for nanotechnology-based biomedical applications.
High avidity IFN-neutralizing antibodies in pharmaceutically prepared human IgG. (1995)

This paper demonstrates and characterizes naturally occurring antibodies to interferon (IFN) in human IgG preparations. In vitro neutralization of the antiviral effect of IFN alpha and IFN beta, but not IFN gamma, was observed in 12 of 15 normal IgG preparations. The neutralizing capacity was higher against rIFN alpha 2A and rIFN alpha 2C than against lymphoblastoid IFN alpha and IFN beta. Frühsommer meningoencephalitis hyperimmune IgG and hepatitis-B hyperimmune IgG showed potent neutralization, whereas anti-rhesus D-, anti-rabies-, and anti-tetanus IgG showed weak neutralization. Saturable binding of 125I-rIFN alpha 2A was demonstrated only in those IgG preparations found to neutralize the antiviral effect of IFN. Significant correlation between IFN binding and neutralization capacity was observed. The antibodies bound with Fab to rIFN alpha 2A with an avidity of approximately 30 pM; the majority was of the IgG1 subclass. Maximum binding capacity was 490 pg rIFN alpha 2A/mg IgG. Cross-binding of rIFN alpha 2C, lyIFN alpha N1 and IFN beta occurred with 10 and 100-200 times lower activities than that of rIFN alpha 2A. There was no cross-binding with rIFN gamma or rIL-6. IgG preparations containing anti-IFN antibodies blocked the binding of 125I-rIFN alpha 2A to A549 cells. In conclusion, pharmaceutically prepared human IgG preparations contain variable but significant levels of high-avidity IFN alpha and IFN beta neutralizing antibodies.
Applicability of an EM-CCD for spatially resolved TIR-ICS (2010)

In this work we systematically explored performance of an EM-CCD as a detector for spatially resolved total internal reflection image correlation spectroscopy (TIR-ICS) with respect to adjustable parameters. We show that variations in the observation volume (pixel binning) can be well described by a simple structural term omega. To test the sensitivity of camera-based TIR-ICS we measured diffusion coefficients and particle numbers (PN) of fluorescent probes of different sizes (Fluorospheres, GFP and labeled antibodies) at varying viscosities, concentrations, and sampling rates. TIR-ICS allowed distinguishing between different probe concentrations with differences in PN of 5% and differences of 6% in D by acquiring only 15 independent measurement runs.
1,3-Diazepinones. 1. Synthesis of 5-hydroxyperhydro-1,3-diazepin-2-one (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1,3-Diazepinones. 1. Synthesis of 5-hydroxyperhydro-1,3-diazepin-2-oneVictor E. Marquez, Paul S. Liu, James A. Kelley, and John S. DriscollCite this: J. Org. Chem. 1980, 45, 3, 485–489Publication Date (Print):February 1, 1980Publication History Published online1 May 2002Published inissue 1 February 1980https://pubs.acs.org/doi/10.1021/jo01291a022https://doi.org/10.1021/jo01291a022research-articleACS PublicationsRequest reuse permissionsArticle Views516Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cytologic evidence for gene amplification in methotrexate-resistant cells obtained from a patient with ovarian adenocarcinoma. (1984)

To analyze if methotrexate (MTX) resistance arises from gene amplification in a patient treated clinically with MTX, the cytogenetic and drug sensitivity profile of the tumor colony forming units (TCFUs) from a 58-year-old woman with stage III well-differentiated ovarian serous adenocarcinoma was studied. This patient had not received treatment directed against her tumor for nine months before this study, but had received oral-dose MTX (2.5 mg, twice weekly) for three years for the treatment of psoriasis. Analysis of TCFUs grown in nucleoside-free media demonstrated MTX resistance at concentrations of up to 100 micrograms/mL (2.2 X 10(-4)M). Cytologic evidence for dihydrofolate reductase (DHFR) gene amplification in TCFUs was determined by in situ hybridization, using radiolabeled cDNA to DHFR mRNA. Results localized the DHFR sequences to an abnormally staining region present on chromosome 4q. This study supports the notion that alterations in gene dosage (that is, gene amplification) play a role in the development of drug resistance in spontaneous human cancers.
Reactions of some aromatic nitro compounds with alkali metal amides (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of some aromatic nitro compounds with alkali metal amidesSham S. Gandhi, Martin S. Gibson, Magdy L. Kaldas, and S. Martin VinesCite this: J. Org. Chem. 1979, 44, 25, 4705–4707Publication Date (Print):December 1, 1979Publication History Published online1 May 2002Published inissue 1 December 1979https://pubs.acs.org/doi/10.1021/jo00393a052https://doi.org/10.1021/jo00393a052research-articleACS PublicationsRequest reuse permissionsArticle Views569Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Immunization with virus-modified tumor cells. (1998)

Direct infection of tumor cells with viruses transfering protective or therapeutic genes-a frequently used procedure for production of tumor vaccines in human gene therapy-is often limited by the number of tumor cells that can reliably be infected, as well as by issues of selectivity and safety. In this review, we describe an efficient, selective, and safe way of infecting human tumor cells with a natural virus with interesting pleiotropic immune stimulatory properties, the avian paramyxovirus Newcastle disease virus (NDV). Advantages of this virus are its good cell-binding properties, its selective replication in tumor cell cytoplasm, which is independent of cell proliferation, and its relative safety. Most important for its use as an adjuvant in human cancer vaccine are its ability to introduce T-cell costimulatory activity, to prevent anergy induction, and to induce locally chemokines (eg, RANTES, IP-10) and cytokines (eg, interferon alpha, beta [IFN-alpha, beta] and tumor necrosis factor-alpha [TNFalpha]) that affect T-cell recruitment and activation. A further development consists of attachment-via NDV-derived hemagluttinin-neuraminidase (HN) membrane-anchoring molecules-of universal defined bispecific reagents such as T-cell-activating anti-CD28 antibodies. Finally, we summarize the status of our clinical studies with the autologous virus modified live cell vaccine (ATV)-NDV.