gebhart/patrepeval huggingface dataset:
Tumor necrosis factor-receptor 2 is up-regulated on lamina propria T cells in Crohn's disease and promotes experimental colitis in vivo (2002)

Tumor necrosis factor (TNF) plays a pivotal role in the pathogenesis of Crohn's disease (CD). However, little is known about the role of TNF receptors (TNF-R) in this disease. Here, we found that TNF-R2 (in contrast to TNF-R1) was significantly up-regulated on lamina propria and peripheral blood T cells in CD compared to control patients. To directly test the functional role of TNF-R2 in Th1-mediated experimental colitis in vivo, we took advantage of transgenic animals overexpressing TNF-R2 in T cells. Reconstitution of SCID mice with CD4+ CD62L+ T cells from TNF-R2 transgenic mice led to an earlier wasting syndrome, a more severe colitis and augmented Th1 cytokine production than reconstitution with cells from wild-type littermates. In addition, TUNEL staining revealed a significantly decreased apoptosis rate of lamina propria mononuclear cells in mice reconstituted with TNF-R2 transgenic T cells compared to mice reconstituted with wild-type T cells. In summary, our data suggest a critical regulatory role of TNF-R2 signaling for disease exacerbation in Th1-mediated chronic colitis. Taken together with the increased expression of TNF-R2 in CD, selective targeting of TNF-R2 signaling thus emerges as a potentially novel approach to the treatment of CD.
Combination Immunotherapy after ASCT for Multiple Myeloma Using MAGE-A3/Poly-ICLC Immunizations Followed by Adoptive Transfer of Vaccine-Primed and Costimulated Autologous T Cells (2014)

A high frequency of vaccine-specific T-cell responses were generated after transplant by combining costimulated autologous T cells with a Poly-ICLC/GM-CSF-primed MAGE-A3 vaccine.
AN INVESTIGATION OF THE NATURAL MICROSCALE MECHANISMS THAT CAUSE VOLUME CHANGE IN EXPANSIVE CLAYS (1977)

The report concludes a study of the natural microscale mechanisms which cause volume change in expansive soils. The mechanisms were defined and attempts were made to delineate the roles of the mechanisms in the volume change process by inferring the respective influence from observed physical, physicochemical, and mineralogical data. The three major microscale mechanisms which cause the volume change are clay particle attraction, cation hydration, and osmotic repulsion. These three major mechanisms can be represented by the total soil suction which is composed of two components, matrix and osmotic. The matrix component represents the clay particle attraction and cation hydration mechanisms. The osmotic component represents the osmotic repulsionmechanism. The results of the laboratory study concluded that although interesting trends may be infered using the approach, it is not successful to infer microscale bahavior from macroscale observations. For the materials and range of moisture contents tested it was concluded that hte clay particle attraction and cation hydration mechanisms play the major role in causing volume change. At higher moisture contents and higher cation concentration environments the osmotic repulsion mechanism provides a secondary influence on volume change. The thermocouple psychrometric techniqe was shown to be a simple, quick, and reliable method for evaluating soil suction. /FHWA/
The effect of 2-methoxyoestrone-3-O-sulphamate on the growth of breast cancer cells and induced mammary tumours (2000)

2-Methoxyoestrogens are emerging as a new class of drug that can inhibit tumour growth and angiogenesis. As sulphamoylation of oestrogens enhances their potency and bioavailability we have synthesized 2-methoxyoestrone-3-O-sulphamate (2-MeOEMATE) and compared its ability to inhibit the proliferation of breast cancer cells with that of 2-methoxyoestrone (2-MeOE1). 2-MeOEMATE (1 microM) inhibited the growth of oestrogen receptor positive MCF-7 breast cancer cells by 52% whereas 2-MeOE1 had little effect at this concentration. 2-MeOEMATE also inhibited the growth of oestrogen receptor negative MDA-MB-231 breast cancer cells. Exposure of cells to 2-MeOEMATE caused them to round up and become detached suggesting that this compound may induce cells to undergo apoptosis. Cell cycle analysis revealed that 2-MeOEMATE caused cells to arrest in the G(2)/M phase with the increase in G(2)/M arrested cells being detectable by 12 hr. Exposure of MCF-7 cells to 2 L-MeOEMATE for 24 hr followed by culture in drug-free medium for 24 hr did not reverse the arrest of cells in the G(2)/M phase. TUNEL analysis confirmed that 2-MeOEMATE induced apoptosis in a significant proportion of treated MCF-7 cells. In an in vivo study, employing nitrosomethylurea-induced mammary tumours in intact rats, 2-MeOE1 (20mg/kg/d, p.o. for 11 days) had little effect on tumour growth. In contrast, the same dose of 2-MeOEMATE resulted in the almost complete regression of 2/3 tumours over an 11-day period. We conclude that 2-MeOEMATE should have considerable therapeutic potential for the treatment of breast tumours.
Field-of-view-dependent helical pitch in multislice CT (2001)

A goal of multi-scale CT (MSCT) is to decrease the scan time needed to image a volume of the patient. Detectors with multiple rows of sensors enable higher helical pitches and thus shorter scan times. One of the difficulties in devising an efficient reconstruction method with good image quality and good dose utilization is that each image pixel is irradiated by the cone-beam for a different range of gantry orientations. We derive a new half-scan weighting scheme for a helical, cone-beam backprojection algorithm based on the virtual fan angle. The virtual fan angle, in turn, determines the gantry view range such that image quality is maintained by allowing only valid ray-sums while using the available dose. This restricts the virtual fan angle to be at least the true geometric fan angle but less than 180 degree(s). The result is a computational efficient and dose efficient reconstruction algorithm with a continuous range of field-of-view dependent helical pitches. A 43% higher helical pitch is possible for the smallest field-of-view compared with the largest field-of-view, using the parameters of a commercial MSCT-scanner. Dose efficiency is compared among the new method and standard half scan and full scan approaches.
Basic Fibroblast Growth Factor (FGF-2) Overexpression Is a Risk Factor for Esophageal Cancer Recurrence and Reduced Survival, which Is Ameliorated by Coexpression of the FGF-2 Antisense Gene (2005)

Overexpression of FGF-2 mRNA is associated with tumor recurrence and reduced survival after surgical resection of esophageal cancer and that these risks are reduced in tumors coexpressing the FGF-AS mRNA. These data support the hypothesis that FGF-AS is a novel tumor suppressor that modulates the effect of FGF-2 expression and may have potential clinical application to the development of novel therapeutic strategies.
Functional analysis of sequences adjacent to dapE of Corynebacterium glutamicum reveals the presence of aroP, which encodes the aromatic amino acid transporter (1995)

An initially nonclonable DNA locus close to a gene of L-lysine biosynthesis in Corynebacterium glutamicum was analyzed in detail. Its stepwise cloning and its functional identification by monitoring the amino acid uptakes of defined mutants, together with mechanistic studies, identified the corresponding structure as aroP, the general aromatic amino acid uptake system.
Regulation of human skin pigmentation and responses to ultraviolet radiation (2006)

Pigmentation of human skin is closely involved in protection against environmental stresses, in particular exposure to ultraviolet (UV) radiation. It is well known that darker skin is significantly more resistant to the damaging effects of UV, such as photocarcinogenesis and photoaging, than is lighter skin. Constitutive skin pigmentation depends on the amount of melanin and its distribution in that tissue. Melanin is significantly photoprotective and epidermal cells in darker skin incur less DNA damage than do those in lighter skin. This review summarizes current understanding of the regulation of constitutive human skin pigmentation and responses to UV radiation, with emphasis on physiological factors that influence those processes. Further research is needed to characterize the role of skin pigmentation to reduce photocarcinogenesis and to develop effective strategies to minimize such risks.
A history of automated highway systems in Japan and future issues (2008)

This work describes research activities on automated highway systems (AHS) since the 1960's conducted in Japan mainly in the Mechanical Engineering Laboratory (MEL) and the National Institute of Advanced Industrial Science and Technology (AIST), the successor of MEL, under Japanese Ministry of Economy, Trade and Industry (METI), and introduces a new project on AHS started in 2008 with the aim of energy saving or global warming prevention by METI. The research institute has been continuously conducting the research since 1960's till now. The system in 1960's employed inductive cable embedded under a roadway surface for lateral control, while the system in 1970's was a vision-based one, which was extended to have an autonomous navigation function with dead reckoning in 1980’s. The autonomous navigation function was improved to use RTK-GPS and five autonomous vehicles linked with the inter-vehicle communication drove under flexible cooperative platooning in 2000. The AHS project started in 2008 will deal with a heavy truck platoon along an expressway, and a light truck along urban streets under mixed traffic. Besides the above activities, Japanese Ministry of Construction demonstrated AHS in 1995 and 1996 with magnetic markers on a roadway, and some automotive companies conducted research during 1990's. Automated transit buses by Toyota, named IMTS (Intelligent Multimodal Transit System), were operated on a theme park and at the World Fare in 2005 along a dedicated lane, and it was a very few case of AHS operated in a real world.
Crab Shell Chitin Whisker Reinforced Natural Rubber Nanocomposites. 2. Mechanical Behavior (2003)

In a previous work (part 1), nanocomposite materials were obtained using a latex of either unvulcanized or prevulcanized natural rubber as the matrix and a colloidal suspension of crab chitin whiskers as the reinforcing phase. The mechanical behavior of the resulting nanocomposite films was analyzed in both the linear and the nonlinear range in the present study. The effects of the filler and processing technique were evaluated, and the results are discussed based on the knowledge of the structural morphology and swelling behavior reported in our previous work. The reinforcing effect of chitin whiskers strongly depended on their ability to form a rigid three-dimensional network, resulting from strong interactions such as hydrogen bonds between the whiskers. The results emanating from the successive tensile test experiments give clear evidence for the presence of a three-dimensional chitin network within the evaporated samples. Cross-linking of the matrix was found to interfere with the formation of this network.
Immortalized Hypothalamic Neurons Express Metabotropic Glutamate Receptors Positively Coupled to Cyclic AMP Formation (1996)

We have characterized the expression pattern and pharmacological profile of activation of metabotropic glutamate receptors (mGluRs) in immortalized, gonadotropin releasing hormone (GnRH)-secreting GT1-7 cells, which represent a homogeneous cellular population of hypothalamic origin. These cells are known to respond to the mGluR agonist (1S,3R)-cyclopentanedicarboxylic acid (1S,3R-ACPD) with increased GnRH release. To establish which specific mGluR subtypes are expressed by GT1-7 cells, we used polyclonal antibodies raised against non-conserved regions of the carboxy-terminal domains of individual subtypes. The selectivity of these antibodies was tested in HEK 293 cells transiently transfected with each mGluR subtype. GT1-7 cells stained positively for the subtypes mGluR1a, -1b and -5 (belonging to group I mGluR2/3 (group II) and mGluR7 (group III). Agonists of group I mGluRs, including 1S,3R-ACPD, activated phosphoinositide hydrolysis in GT1-7 cells. This effect, however, was manifested only when cell density was low, and it disappeared when cells reached confluence. Stimulation of phosphoinositide hydrolysis could not therefore have been related to hormone secretion because 1S,3R-ACPD effectively released GnRH in confluent cultures. We then focused on group II and III mGluRs, which in transfected cells are negatively linked to adenylate cyclase activity. Unexpectedly, however, agonist which preferentially activate group II and III mGluRs increased both basal and forskolin-stimulated cAMP accumulation in GT1-7 cells. Stimulation of cAMP accumulation by mGluR agonists was not prevented by enzymatic depletion of endogenous adenosine, but was obliterated when cells were incubated with agonists of receptors positively coupled to adenylate cyclase, such as beta-adrenergic and prostaglandin E2 receptors. These results suggest that GT1-7 cells express a novel mGluR subtype positively coupled to adenylate cyclase, which shares the same transduction pathway of other classical receptors coupled with a Gs-type of GTP-binding protein.
Antigen microarray profiling of autoantibodies in rheumatoid arthritis (2005)

Proteomic analysis of autoantibody reactivities provides diagnostic information and allows stratification of patients with early RA into clinically relevant disease subsets.
Effect of selenium and vitamin E supplements on tissue lipids, peroxides, and fatty acid distribution in experimental diabetes (1998)

The protective role of selenium (Se), given as a Se-rich yeast, selenomethionine or selenomethionine + vitamin E supplement, toward changes in lipid, peroxide, and fatty acid distribution in tissues of streptozotocin-induced diabetic rats, was investigated, after 24 wk of disease. Diabetes increased liver thiobarbituric acid-reactive substances and conjugated dienes; Se supplement completely corrected these changes. In kidney, as in heart, the peroxide levels were not significantly changed by diabetes. In diabetic rat liver, a significant drop in triglycerides and phospholipids (P < 0.05) was observed; this was modulated by Se + vitamin E supplementation. Se + vitamin E supplementation also inhibited the decrease in 18:2n-6 and the increase in 22:6n-3 observed in liver of diabetic rats, changes which reflect altered glycemic control. In kidney, heart, and aorta, diabetes produced some changes in lipid content and fatty acid distribution, especially an increase in heart triglycerides which was also corrected by the Se supplement. Se supplementation to diabetic rats also increased 18:0 ether-linked alcohol, 20:4 n-6, and 22:5 n-3 in cardiac lipids. In aorta, Se + vitamin E significantly increased 20:5 n-3. These polyunsaturated fatty acids are precursors, in situ, of prostaglandin I2 (PGI2) and PGI3 which may protect against cardiovascular dysfunction. In kidney, conversely, Se decreased 20:4 n-6, the precursor of thromboxane A2 implicated in diabetic glomerular injury. Thus Se, and more efficiently Se + Vitamin E supplementation, in experimental diabetes could play a role in controlling oxidative status and altered lipid metabolism in liver, thereby maintaining favorable fatty acid distribution in the major tissues affected by diabetic complications.
Supramolecular chemistry of manganese complex [Mn(CO)3(.mu.3-OH)]4: assembly of a cubic hydrogen-bonded diamondoid network with 1,2-diaminoethane (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSupramolecular chemistry of manganese complex [Mn(CO)3(.mu.3-OH)]4: assembly of a cubic hydrogen-bonded diamondoid network with 1,2-diaminoethaneSteven B. Copp, S. Subramanian, and Michael J. ZaworotkoCite this: J. Am. Chem. Soc. 1992, 114, 22, 8719–8720Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/ja00048a066https://doi.org/10.1021/ja00048a066research-articleACS PublicationsRequest reuse permissionsArticle Views402Altmetric-Citations111LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Connecting dysbiosis, bile-acid dysmetabolism and gut inflammation in inflammatory bowel diseases (2012)

Impaired microbiota enzymatic activity observed in IBD-associated dysbiosis leads to modifications in the luminal BA pool composition. Altered BA transformation in the gut lumen can erase the anti-inflammatory effects of some BA species on gut epithelial cells and could participate in the chronic inflammation loop of IBD.
Site‐directed ELISA with synthetic peptides representing the HIV transmembrane glycoprotein (1987)

Two partially overlapping 19 and 22 amino acids long peptides representing a highly immunogenic site of the transmembranous glycoprotein (gp41) of human immunodeficiency virus (HIV) were used as antigen in ELISA tests. The results of antibody determination with this assay were compared with those of three or more conventional ELISAs and Western blot (WB) tests and radioimmunoprecipitation assay. Twenty-six sera from patients with AIDS or LAS and from asymptomatic carriers of HIV infection all showed a pronounced reaction in the peptide ELISA as well as positive results with other tests. In contrast, 27 sera from laboratory workers and blood donors were negative by all tests. A group of 39 blood donor sera, which had shown false positive or ambiguous results in the ELISAs and sometimes in WB tests employed for confirmation, also were negative in all cases with the peptide ELISA. Consecutive samples collected from individuals with primary HIV infection were also analyzed. In 6 out of 9 cases, the peptide ELISA revealed an antibody response within one month after onset of clinical symptoms and sensitivity for antibody detection equaled that of other ELISA tests. Eight sera from five West African persons infected with HIV-related viruses did not react in the peptide ELISA, reflecting differences in properties of the envelope components. The peptide ELISA used in this study appears to represent a simple technique employing chemically synthesized antigen for accurate and sensitive estimation of antibodies to the HIV group of nontransforming human retroviruses.
Chromosome end associations, telomeres and telomerase activity in ataxia telangiectasia cells (1995)

Cells derived from individuals with ataxia telangiectasia (AT) show enhanced spontaneous levels of chromosomal abnormalities and are sensitive to ionizing radiations and radiomimetic drugs, as evidenced by decreased survival and increased chromosome aberration frequencies at mitosis when compared with normal cell lines. The higher base line frequencies of chromosome aberrations in part involve chromosome end-to-end associations as seen at metaphase. Since telomeres of tumor cells and aging tissues are often reduced in length, chromosome end associations may be due to loss of telomeric repeats. We studied the chromosome behavior and telomeres of two ataxia telangiectasia lymphoblastoid cell lines compared to two normal control cell lines. The ataxia telangiectasia cell lines showed higher frequencies of chromosome end associations both at metaphase and in interphase, determined in prematurely condensed chromosomes of G1 and G2 cells. They also showed higher frequencies of chromosomal breaks at metaphase and fewer telomeric signals determined using fluorescent in situ hybridization with a (TTAGGG)n probe. The frequency of telomeric repeats was variable in the ataxia telangiectasia cell lines (4.3 and 8.2 kb) compared to the normal cell lines (9.6 and 12 kb) and an inverse correlation between telomere length and chromosome end associations was observed. Both ataxia telangiectasia cell lines showed more robust telomerase activity than the normal cell lines, precluding defective enzymatic capacity as the basis for the chromosome end associations. It is possible that chromatin structure in the form of telomere-nuclear matrix interactions are variant in ataxia telangiectasia cells negatively influencing telomerase function and contributing to telomere associations.
Sequence motifs important for peptide binding to the human MHC class I molecule, HLA-A2 (1992)

Previous studies have indicated that most HLA-A2-binding peptides are 9 amino acid (aa) residues long, with a Leu at position 2 (P2), and a Val or Leu at P9. We compared the binding properties of different peptides by measuring the rate of dissociation of beta 2-microglobulin from peptide-specific HLA-A2 complexes. The simplest peptide that we identified that could form HLA-A2 complexes had the sequence (in single letter aa code) GLFGGGGGV, indicating that three nonglycine aa are sufficient for binding to HLA-A2. To determine whether most nonapeptides that contained Leu at P2 and Val or Leu at P9 could bind to HLA-A2, we tested the binding of nonapeptides selected from published HIV and melanoma protein sequences, and found that six of seven tested formed stable HLA-A2 complexes. We identified an optimal antigenic undecapeptide from the cytomegalovirus gB protein that could form stable HLA-A2 complexes that contained apparent anchor residues at P2 and P11 (sequence FIAGN-SAYEYV), indicating that the spacing between anchor residues can be somewhat variable. Finally, we tested the importance of every aa in the influenza A matrix peptide 58-66 (sequence GILGFVFTL) for binding to HLA-A2, by using Ala-substituted and Lys-substituted peptides. We found that multiple positions were important for stable binding, including P2, P3, P5-P7, and P9. We conclude that the P2 and P9 anchor residues are of prime importance for peptide binding to HLA-A2. However, other peptide side chains (especially at P3) contribute to the stability of the interaction. In certain cases, the optimal length for peptide binding can be longer than 9 residues.
Optically pumped ultrashort cavity In_1−xGaxAsyP_1−y lasers: picosecond operation between 083 and 159 μm (1981)

Ultrashort-cavity, thin-film lasers from In(1-x)Ga(x)As(y)P(1-y) of five different compositions, including InP and In(0.53)Ga(0.47)As, have been made to lase between 0.83 and 1.59 microm. The multitude of lasing wavelengths observed had line-to-line separations of less than 10 nm. The lasers were pumped with 1-psec pulses from a mode-locked dye laser. An output pulse of 6-psec duration was measured at a wavelength of 1.16 microm.
α-Iminoenamido Ligands:  A Novel Structure for Transition-Metal Activation (2002)

This communication shows that it is possible to activate nickel complexes containing the α-iminoenamide ligand using B(C6F5)3 or Al(C6F5)3 to generate catalysts that polymerize ethylene. The polymerization of ethylene using these novel compounds is discussed. The novel activation method is confirmed by the X-ray crystallography studies of the methallyl analogs.