gebhart/patrepeval huggingface dataset:
A Comparison of Morphine, Fentanyl, and Sufentanil Anesthesia for Cardiac Surgery (1986)

We compared anesthetic doses of three popular opiates, morphine (n = 10), fentanyl (n = 9), and sufentanil (n = 9) in patients undergoing cardiac surgery. Opiate administration after induction was based upon EEG and cardiovascular signs of the depth of anesthesia. Total doses were morphine, 4.4 +/- 0.71 mg/kg, fentanyl, 95.4 +/- 9.9 micrograms/kg, and sufentanil, 18.9 +/- 2.2 micrograms/kg. Comparisons among opiates included times for induction of anesthesia, return of consciousness, return of spontaneous ventilation, return of adequate cardiovascular status, and extubation. The following times (mean and SEM) were significantly (P less than 0.05) shorter for sufentanil than for fentanyl or morphine: induction (15 +/- 2.3 min, 5.9 +/- 0.7 min, and 3.0 +/- 0.2 min for morphine, fentanyl, and sufentanil, respectively); return of consciousness (morphine 109.7 +/- 34.4 min, fentanyl 62.3 +/- 17.9 min, sufentanil 17 +/- 8.7 min); return of acceptable and stable cardiovascular status (morphine 587.3 +/- 139.3 min, fentanyl 537.9 +/- 144.8 min, sufentanil 173.7 +/- 56.8 min); and extubation (morphine 1121.3 +/- 61.8 min, fentanyl 1005.7 +/- 77.7 min, sufentanil 533.3 +/- 67.8 min). We conclude that sufentanil administered in the dosage range of 19 micrograms/kg allows more rapid induction, earlier emergence from anesthesia, and faster extubation of patients than either morphine or fentanyl.
DVORAK and COLEMAK Keyboards (2015)

A previous article investigate typable words from the various rows of keys on the AZERTY and QWERTZ keyboards. In this article, I look at the layouts of the DVORAK and COLEMAK keyboards.
Vaccines Against Polysaccharide Antigens (2001)

Encapsulated bacteria such as Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae serogroup B (Hib) are a major cause of disease worldwide. Vaccine development against these organisms has targeted their capsular polysaccharides (CPS), as anti-capsular antibodies often protect against disease. The capsular polysaccharide vaccines that have been available against these organisms are neither immunogenic nor protective in young children and certain immunocompromised individuals. In general, polysaccharide (PS) antigens elicit a T-independent immune response, characterized by lack of memory, and poor immunogenicity at the extremes of life. Efforts to overcome the poor immunogenicity of CPS vaccines have led to development of conjugate vaccines. By conjugating CPS to carrier proteins it is possible to induce a T-dependent immune response against these antigens. Although conjugate vaccines have been successful against Hib disease, their applicability to multi-serotype/serogroup pathogens like the pneumococcus or the meningococcus is questioned. As a result, alternative vaccines including (1) surface proteins conserved across serotypes/serogroups, (2) peptides that mimic PS antigens and (3) DNA vaccines are presently under investigation. This review will highlight the potential and limitations of both CPS and CPS-conjugate vaccines against encapsulated bacteria as well as alternative strategies against PS antigens.
Topical and systemic glucocorticoid potencies of budesonide, beclomethasone dipropionate and prednisolone in man. (1982)

Topical anti-inflammatory ('intracutaneous vasoconstriction') and systemic glucocorticoid potencies (depression of plasma cortisol) were compared in human volunteers after administration of the glucocorticoids budesonide, beclomethasone dipropionate (BDP) and prednisolone. After topical application budesonide was about twice as potent as BDP and more than 1 000 times more potent than prednisolone and hydrocortisone in inducing 'vasoconstriction'. After oral administration, on the other hand, budesonide was one half to one third as potent as BDP in depressing plasma cortisol. After inhalation budesonide was only half as potent as BDP. When inhaled budesonide was compared to oral prednisolone budesonide 1 600 micrograms and prednisolone 5 mg were shown to have the same effect on plasma cortisol. The improved ratio between the topical and the systemic glucocorticoid effect of budesonide, makes the drug a promising alternative for aerosol treatment in asthma.
Cyr61, product of a growth factor-inducible immediate early gene, is associated with the extracellular matrix and the cell surface. (1991)

cyr61 is a specific target for activation by platelet-derived growth factor and fibroblast growth factor and is inducible by the oncogene v-src. It is a member of the class of immediate early genes that includes those encoding protooncogene products, transcription factors, and cytokines. We have previously characterized the synthesis and degradation of the cyr61-encoded mRNA and protein. Although the deduced Cyr61 protein sequence contains an NH2-terminal secretory signal, it is not detectable in the conditioned medium of serum-stimulated cells. We show here that in rapidly growing cell cultures, newly synthesized Cyr61 is secreted and is associated with both the extracellular matrix and the cell surface. In contrast, Cyr61 secreted in serum-stimulated quiescent cells is directed to the cell surface and is hot incorporated into the extracellular matrix. Once associated with the extracellular matrix, Cyr61 has a half-life of greater than 24 h, whereas intracellular and cell surface-associated Cyr61 has an apparent half-life of approximately 30 min. Furthermore, Cyr61 appears to bind heparin with high affinity. These observations suggest similarities among Cyr61, the fibroblast growth factors (heparin-binding growth factors), and the protooncogene product Int-1 and are consistent with the hypothesis that Cyr61 plays a role in cell-cell communication involving the interaction of neighboring cells.
Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER) (2001)

A new method for SNP analysis based on the detection of pyrophosphate (PPi) is demonstrated, which is capable of detecting small allele frequency differences between two DNA pools for genetic association studies other than SNP typing. The method is based on specific primer extension reactions coupled with PPi detection. As the specificity of the primer-directed extension is not enough for quantitative SNP analysis, artificial mismatched bases are introduced into the 3'-terminal regions of the specific primers as a way of improving the switching characteristics of the primer extension reactions. The best position in the primer for such artificial mismatched bases is the third position from the primer 3'-terminus. Contamination with endogenous PPi, which produces a large background signal level in SNP analysis, was removed using PPase to degrade the PPi during the sample preparation process. It is possible to accurately and quantitatively analyze SNPs using a set of primers that correspond to the wild-type and mutant DNA segments. The termini of these primers are at the mutation positions. Various types of SNPs were successfully analyzed. It was possible to very accurately determine SNPs with frequencies as low 0.02. It is very reproducible and the allele frequency difference can be determined. It is accurate enough to detect meaningful genetic differences among pooled DNA samples. The method is sensitive enough to detect 14 amol ssM13 DNA. The proposed method seems very promising in terms of realizing a cost-effective, large-scale human genetic testing system.
<title>Rate-matching packet scheduler for real-rate applications</title> (2000)

A packet scheduler is an operating system component that controls the allocation of network interface bandwidth to outgoing network flows. By deciding which packet to send next, packet schedulers not only determine how bandwidth is shared among flows, but also play a key role in determining the rate and timing behavior of individual flows. The recent explosion of rate and timing-sensitive flows, particularly in the context of multimedia applications, has focused new interest on packet schedulers. Next generation packet schedulers must not only ensure separation among flows and meet real-time performance constraints, they must also support dynamic fine- grain real-location of bandwidth for flows with variable-bit- rate requirements. Unfortunately, today's packet schedulers either do not support rate and timing sensitive flows, or do so with reservation systems that are relatively coarse-grain and inflexible. This paper makes two contributions. First it shows how bandwidth requirements can be inferred directly from real-rate flows, without requiring explicit specifications from the application. Second, it presents the design, implementation and performance evaluation of a rate-matching packet scheduler that uses these inferred requirements to automatically and dynamically control the bandwidth allocation to flows.
Quantitative Micromethod for Determination of Phenylalanine and Tyrosine in Blood and Its Application in the Diagnosis of Phenylketonuria in Infants (1963)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Bispecific Antibody Mediated Targeting of nido-Carboranes to Human Colon Carcinoma Cells (1996)

Boron neutron capture therapy, a binary form of cancer treatment, has the potential to deliver potent cytotoxic radiation to tumor cells with minimal collateral damage to normal tissues if methods for the selective accretion of elevated concentrations of boron-10 in tumor can be developed. In this regard, a monoclonal antibody with dual specificity, for both anionic boron cluster compounds (nido-carboranes) and a tumor-associated antigen (carcinoembryonic antigen, CEA), was produced. The specific binding of a nido-carborane to CEA-expressing tumor cells was achieved using this bispecific antibody. The ability of this bispecific antibody to concentrate selectively at tumor sites in vivo has also been demonstrated, thus suggesting its potential for sequestering boron-rich compounds in tumors.
High-affinity binding sites for related fibroblast growth factor ligands reside within different receptor immunoglobulin-like domains. (1994)

Growth factors of the fibroblast growth factor (FGF) family bind receptors whose external domains are organized in a series of immunoglobulin-like loops. We engineered expression constructs in which cDNAs encoding individual immunoglobulin-like domains of the keratinocyte growth factor (KGF/FGF-7) receptor were fused to the mouse immunoglobulin heavy chain Fc domain (HFc). Each chimera was efficiently secreted from NIH 3T3 transfectants and migrated at the predicted molecular mass after SDS/PAGE. Scatchard analysis revealed that the chimera containing immunoglobulin-like domains 2 (D2) and 3 (D3) bound KGF and acidic FGF at high affinities comparable to the native receptor. However, individual immunoglobulin-like domain chimeras demonstrated marked specificity in their ligand interactions. D2-HFc bound acidic FGF at high affinity, whereas it did not detectably interact with KGF. Conversely, D3-HFc bound KGF at high affinity but exhibited no detectable interaction with acidic FGF. Their selective ligand binding properties were confirmed by the specific neutralization of acidic FGF or KGF mitogenic activity using D2 or D3 HFc, respectively. All of these findings establish that the major binding sites for related FGF ligands are localized to distinct receptor immunoglobulin-like domains.
SIRPα-Antibody Fusion Proteins Selectively Bind and Eliminate Dual Antigen-Expressing Tumor Cells (2016)

These findings establish SIRPabodies as a promising approach to deliver the therapeutic benefit of CD47-SIRPα blockade specifically toward tumor cells. SIRPabodies may be applied to additional cancer types by grafting SIRPα onto other tumor-specific therapeutic antibodies. Clin Cancer Res; 22(20); 5109-19. ©2016 AACR.
High-power HTS microstrip filters for wireless communication (1995)

The performance of narrowband microstrip filters with low insertion loss and high power-handling capabilities made from YBa/sub 2/Cu/sub 3/O/sub 7-/spl delta// (YBCO) high-temperature superconducting (HTS) thin films is presented. Results are shown for two different designs that were chosen to optimize the power-handling capability. Both filters have a 2-GHz center frequency and 5 poles that incorporate coupled resonators with 10-/spl Omega/ internal impedances. They were made on 5-cm-diameter LaAlO/sub 3/ substrates. Both designs use parallel-coupled feed lines to avoid current crowding, The first design includes backward- and forward-coupled filters, has 1% bandwidth, and has handled over 25 watts of input power at 10 K with less than 0.25 dB compression. The second design has 1.2% bandwidth and uses only forward-coupled resonators. The dissipation loss is less than 0.2 dB at 45 K and it has a third-order intercept of 62 dBm. Another similar filter handled 36 watts of power at 45 K with less than 0.15 dB compression across the passband. We have developed a technique to visualize the power dissipation of the filter by observing the bubbles created by the filter when submerged in liquid helium, showing areas with local defects or where the current distribution is at its peak value. We also discuss several planar high-power filter issues, including material selection and fabrication, device configuration trade-offs, filter structure optimization, and design approaches to maximize power-handling capacity.
Salmonella enterica Serovar Enteritidis Core O Polysaccharide Conjugated to H:g,m Flagellin as a Candidate Vaccine for Protection against Invasive Infection withS.Enteritidis (2011)

Nontyphoidal Salmonella enterica serovars Enteritidis and Typhimurium are a common cause of gastroenteritis but also cause invasive infections and enteric fever in certain hosts (young children in sub-Saharan Africa, the elderly, and immunocompromised individuals). Salmonella O polysaccharides (OPS) and flagellar proteins are virulence factors and protective antigens. The surface polysaccharides of Salmonella are poorly immunogenic and do not confer immunologic memory, limitations overcome by covalently attaching them to carrier proteins. We conjugated core polysaccharide-OPS (COPS) of Salmonella Enteritidis lipopolysaccharide (LPS) to flagellin protein from the homologous strain. COPS and flagellin were purified from a genetically attenuated (ΔguaBA) "reagent strain" (derived from an isolate from a patient with clinical bacteremia) engineered for increased flagellin production (ΔclpPX). Conjugates were constructed by linking flagellin monomers or polymers at random COPS hydroxyls with various polysaccharide/protein ratios by 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) or at the 3-deoxy-d-manno-octulosonic acid (KDO) terminus by thioether chemistry. Mice immunized on days 0, 28, and 56 with COPS-flagellin conjugates mounted higher anti-LPS IgG levels than mice receiving unconjugated COPS and exhibited high antiflagellin IgG; anti-LPS and antiflagellin IgG levels increased following booster doses. Antibodies generated by COPS-flagellin conjugates mediated opsonophagocytosis of S. Enteritidis cells into mouse macrophages. Mice immunized with flagellin alone, COPS-CRM₁₉₇, or COPS-flagellin conjugates were significantly protected from lethal challenge with wild-type S. Enteritidis (80 to 100% vaccine efficacy).
Evaluation of images from an image mode laser projection system (2003)

Summary form only given. Image mode lasers, which consist of a voltage-controlled pixilated variable loss element placed inside a high-gain laser cavity, have been demonstrated as a feasible method for video projection display. Two methods of creating this cavity loss are examined: the scattering polymer-dispersed liquid crystal (PDLC), and the absorptive twisted nematic (IN) liquid crystal element. The effects of amplified spontaneous emission (ASE) and how they relate to the choice of the loss element are discussed. Evaluation and comparison of 1/4 VGA resolution lasing images to a commercial projection display is also presented.
Microwave-Accelerated Metal-Enhanced Fluorescence:  Platform Technology for Ultrafast and Ultrabright Assays (2005)

We describe an exciting assay platform technology that promises to fundamentally address two underlying physical constraints of modern assays and immunoassays, namely, assay sensitivity and rapidity. By combining the use of metal-enhanced fluorescence with low-power microwave heating, we can indeed significantly increase the sensitivity of surface assays as well as >95 % kinetically complete the assay within a few seconds. Subsequently, this new technology promises to fundamentally change the way we currently employ immunoassays in clinical medicine. This new model platform system can be potentially applied to many other important assays, such as to the clinical assessment of myoglobin, where both assay speed and sensitivity is paramount for the assessment and treatment of acute myocardial infarction. To demonstrate the utility of microwave-accelerated metal-enhanced fluorescence (MAMEF), we show that a simple protein-based assay system can be optically amplified approximately 10-fold by using silver nanostructures, while being kinetically complete in less than 20 s. This new platform approach is subsequently over 10-fold more sensitive and approximately 90 times faster than a control assay that operates both at room temperature and without the use of metal-enhanced fluorescence. Finally, we show that low-power heating by microwaves in our model system does not denature proteins, as evidenced by no protein structural changes, probed by fluorescence resonance energy transfer.
Highly Selective Directional Atomic Layer Etching of Silicon (2015)

Following Moore's Law, feature dimensions will soon reach dimensions on an atomic scale. For the most advanced structures, conventional plasma etch processes are unable to meet the requirement of atomic scale fidelity. The breakthrough that is needed can be found in atomic layer etching or ALE, where greater control can be achieved by separating out the reaction steps. In this paper, we study selective, directional ALE of silicon using plasma assisted chlorine adsorption, specifically selectivities to bulk silicon oxide as well as thin gate oxide. Possible selectivity mechanisms will be discussed.
From Gene to HSQC in under Five Hours:  High-Throughput NMR Proteomics (2006)

A simple, rapid, in vitro cell-free protein expression system, Expressway NMR, is introduced and used to express the small ubiquitin-related modifier protein SUMO-1. This 12 kDa molecule is challenging for NMR as it has limited solubility and requires relatively high salt (200 mM) for stability in solution. Starting with the gene, the cell-free system, and milligram amounts of nitrogen-15 isotopically enriched amino acids, sufficient protein is produced in 4 h to obtain a high-resolution 2D HSQC spectrum of the protein in 40 min. This time would be closer to 10 min with the aid of a higher sensitivity salt-tolerant cryogenic NMR probe. With all protein purification steps included, and aggressive data processing using the filter diagonalization method (FDM), it is but 6 h from gene to heteronuclear single quantum coherence (HSQC). As the cell-free system is nearly background-free, it is also possible to work with the crude reaction mixture, in which case only a total of 5 h is required. Sample stability over time, whether crude extract or purified, was notable, with no significant change in the 15N-1H HSQC spectrum over 6 months at 4 degrees C (300 muM, pH 6.1, capped NMR tube). The combination of a turnkey, high-yield, protease-free in vitro protein expression system, an optimized sensitivity-enhanced HSQC pulse sequence, and FDM processing makes this scheme an attractive first step to rapidly assess the suitability of proteins for complete solution structure determination.
The Changes of Mac-1 and L-Selectin Expression on Granulocytes and Soluble L-Selectin Level during Hemodialysis (1996)

L-selectin and Mac-1 expressed on leukocytes are critical for leukocyte adhesion to inflamed endothelium. L-selectin is known to be rapidly shed from the cell surface of granulocytes after activation. In the present study the change of expressions of these adhesion molecules on granulocytes were analyzed by flow cytometry, and the serum concentration of shed L-selectin (soluble L-selectin; sL-selectin) was measured by enzyme-linked immunosorbent assay (ELISA) during hemodialysis in patients treated with regenerated cellulose membranes (RC group) versus polysulfone membranes (PS group). In the RC group, Mac-1 expression on granulocytes increased significantly at 30 min after the initiation of hemodialysis (p < 0.05) compared with predialysis values, coinciding with the nadir of dialysis-induced granulocytopenia. Granulocyte L-selectin expression decreased significantly at 15 min after the initiation of hemodialysis (p < 0.05) and remained decreased through the course of dialysis session, compared with predialysis values. Serum sL-selectin level significantly increased at 15 min after the initiation of hemodialysis (p < 0.05), compared with predialysis values. In the PS group, no significant variation in Mac-1 and L-selectin expression on granulocytes and serum sL-selectin level were detected. This reciprocal change of Mac-1 and L-selectin on granulocyte cell surface was attributed to development of granulocytopenia and subsequent reversal during dialysis with cellulose membranes. In this study, we confirmed the shedding of L-selectin during cellulosic dialysis by ELISA. The increase in sL-selectin, which has potential activity of inhibiting L-selectin-dependent adhesion of granulocyte to endothelium, might be involved in rebound granulocytosis during dialysis with cellulose membranes and impairment of the granulocyte function in patients on chronic hemodialysis.
Interferon-alpha-2b immunoconjugate for improving immunoscintigraphy and immunotherapy. (2000)

A pretreatment with IFN-MAb conjugate is a worthwhile approach to consider in radioimmunoscintigraphy and RIT.