gebhart/patrepeval huggingface dataset:
Continuous Subcutaneous Heparin Infusion for Treatment of Trousseau's Syndrome (1995)

Continuous subcutaneous heparin infusion may be an effective option for the treatment of Trousseau's syndrome in the ambulatory setting.
Tissue Engineering: Engineering Principles for the Design of Replacement Organs and Tissues (2004)

PART 1: TISSUE EXCHANGE AND TISSUE DEVELOPMENT 1. The State-of-the-Art in Tissue Exchange 2. Objectives of Tissue Engineering 3. Elements of Tissue Development PART 2: TISSUE ENGINEERING FUNDAMENTALS 4. Cell Growth and Differentiation 5. Cell and Tissue Mechanics 6. Cell Adhesion 7. Cell Migration 8. Cell Aggregation and Tissue Equivalents 9. Tissue Barriers to Molecular and Cellular Transport PART 3: TISSUE ENGINEERING PRACTICE 10. Cell Delivery and Recirculation 11. Delivery of Molecular Agents in Tissue Engineering 12. Cell Interactions With Polymers 13. Approaches to Tissue Engineering 14. Case Studies in Tissue Engineering Appendices A. Introduction to Polymers B. Analysis of Molecular Transport C. Useful Data D. Nomenclature and Abbreviations
HOW DO CORROLES STABILIZE HIGH VALENT METALS? (2006)

Trianionic corroles are readily oxidized, yet they are able to stabilize metals in high oxidation states. In an attempt to solve this apparent puzzle, we have estimated both metal d and corrole π orbital energies by analysis of well established experimental data. Although the corrole π orbitals are at higher energies than those of closely related porphyrins, the d orbital energies are even higher, owing to greatly enhanced σ bonding dictated by the relatively rigid macrocyclic trianionic—ligand framework. Understanding the relative importance of corrole π and σ effects sheds new light on metallocorrole stability and reactivity that in turn will further advance this hot area of chemistry.
Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients (1999)

The clinical significance of detecting supposed tumour cell-derived mRNA transcripts in blood using the polymerase chain reaction (PCR) remains unclear. We have used a fully quantitative 5'-nuclease RT-PCR assay to screen for the expression of cytokeratins (ck) 19 and 20 and guanylyl cyclase C (GCC) in the peripheral blood of 21 healthy controls and 27 colorectal cancer patients. Expression of cytokeratin 19 and 20 mRNA was detected in 30% and 100% of samples, respectively, taken from healthy volunteers. There was no apparent difference in ck19 and ck20 mRNA transcription levels between controls and patients, or between patients with different Dukes' stages. While GCC mRNA was detected in only 1/21 control samples, it was expressed in approximately 80% of patients, although again there was no correlation between GCC levels and disease stage. Transcription levels of all three markers varied considerably between samples, even between samples taken from the same person at different times. We conclude that neither ck19 nor ck20 are reliable markers for the detection of colon epithelial cells in peripheral blood and that an evaluation of the usefulness of GCC awaits further longitudinal studies.
Influence of the route of administration on targeting of ovarian cancer with the chimeric monoclonal antibody MOv18: i.v.vs. i.p. (2001)

MOv18 antibody binds the membrane folate receptor highly expressed on ovarian carcinoma cells. Since ovarian cancer is mainly limited to the peritoneal cavity, locoregional delivery of therapeutics can be an option. The same patient was injected i.v. and i.p. with c-MOv18 IgG labeled with different radionuclides. To study the kinetics of c-MOv18, patients were divided into 2 groups. Fifteen patients received c-MOv18 labeled with (131)I, (125)I and (123)I (for imaging). Seven patients were operated 2 days, 7 patients 6 days and 1 patient 3 days post-injection. Radioactivity was determined in blood, ascites and biopsies of tumor and of several normal tissues. No adverse events occurred. No anti-MOv18 responses were observed. The area under the blood activity vs. time curve (AUC) was significantly lower after i.p. injection for 2 and 6 days post-injection (p = 0.01 and p = 0.02, respectively). At 2 days post-injection, a significant difference in tumor uptake was found in favor of the i.v. route of administration (4.9% and 2.4%ID/kg for i.v. and i.p., respectively; p < 0.0001). Uptake in solid tumor tissue in ovarian cancer patients operated 6 days post-injection was not significantly different (p = 0.79) for both routes (3.8% and 3.9%ID/kg for i.v. and i.p., respectively). In conclusion, no advantage could be demonstrated for the i.p. route with respect to tumor uptake. The i.p. route could be advantageous with respect to bone marrow toxicity since the AUC was significantly lower for the i.p. route. However, within 2 days post-injection, the blood clearance followed the same pattern for both routes.
4-1BB Promotes the Survival of CD8+ T Lymphocytes by Increasing Expression of Bcl-xL and Bfl-1 (2002)

4-1BB, a T cell costimulatory receptor, prolongs CD8(+) T cell survival. In these studies, 4-1BB stimulation was shown to increase expression of the antiapoptotic genes bcl-x(L) and bfl-1 via 4-1BB-mediated NF-kappaB activation. This signaling pathway was specifically inhibited by PDTC and was different from the pathways that enhanced CD8(+) T cell proliferation. The results suggest a role for the antiapoptotic activities of Bcl-x(L) and Bfl-1 proteins in 4-1BB-mediated CD8(+) T cell survival in vivo.
Kinetic Evidence of the Regeneration of Multilineage Hematopoiesis From Primitive Cells in Normal Human Bone Marrow Transplanted Into Immunodeficient Mice (1997)

Based on initial observations of human CD34+ Thy-1+ cells and long-term culture-initiating cells (LTC-IC) in the bone marrow of some sublethally irradiated severe combined immunodeficient (SCID) mice transplanted intravenously with normal human marrow cells, and the subsequent finding that the NOD/LtSz-scid/scid (NOD/SCID) mouse supports higher levels of human cell engraftment, we undertook a series of time course experiments to examine posttransplant changes in the number, tissue distribution, cycling activity, and in vivo differentiation pattern of various human hematopoietic progenitor cell populations in this latter mouse model. These studies showed typical rapid posttransplant recovery curves for human CD34- CD19+ (B-lineage) cells, CD34+ granulopoietic, erythroid, and multilineage colony-forming cells (CFC), LTC-IC, and CD34+ Thy-1+ cells from a small initial population representing <0.1% of the original transplant. The most primitive human cell populations reached maximum values at 5 weeks posttransplant, after which they declined. More mature cell types peaked after another 5 weeks and then declined. A 2-week course of thrice weekly injections of human Steel factor, interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and erythropoietin (administered just before the mice were killed for analysis) did not alter the pace of regeneration of either primitive or mature human hematopoietic cells, or their predominantly granulopoietic and B-lymphoid pattern of differentiation, although a significant enhancing effect on the level of human cell engraftment sustained after 3 months was noted. Cycling studies showed the human CFC present at 4 to 5 weeks posttransplant to be rapidly proliferating even in mice not given human growth factors. However, by 10 weeks and thereafter, only quiescent human CFC were detected; interestingly, even in mice that were given the 2-week course of growth factor injections. These studies indicate the use of this model for future analysis of the properties and in vivo regulation of primitive human hematopoietic cells that possess in vivo repopulating ability.
The complement-inhibitory activity of CD59 resides in its capacity to block incorporation of C9 into membrane C5b-9. (1990)

A human E membrane protein that inhibits lysis by the purified human C5b-9 proteins was isolated and characterized. After final purification, the protein migrated as an 18- to 20-kDa band by SDS-PAGE. Elution from gel slices and functional assay after SDS-PAGE (nonreduced) confirmed that all C5b-9 inhibitory activity of the purified protein resided in the 18- to 20-kDa band. Phosphatidylinositol-specific phospholipase C digestion of the purified protein abolished 50% of its C5b-9 inhibitory activity, and removed approximately 15% of the protein from human E. Western blots of normal and paroxysmal nocturnal hemoglobinuria E revealed an absence of the 18- to 20-kDa protein in the paroxysmal nocturnal hemoglobinuria E cells. The identity of this E protein with leukocyte Ag CD59 (P18, HRF20) was confirmed immunochemically and by N-terminal amino acid sequence analysis. A blocking antibody raised against the purified protein reacted with a single 18- to 20-kDa band on Western blots of human erythrocyte membranes. Prior incubation of human E with the F(ab) of this antibody increased subsequent lysis by the purified human C5b-9 proteins. Potentiation of C5b-9-mediated lysis was observed when erythrocytes were preincubated with this blocking antibody before C5b-9 assembly was initiated, or, when this antibody was added after 30 min, 0 degrees C incubation of C5b-8-treated E with C9. Chicken E incubated with purified CD59 were used to further characterize the mechanism of its C-inhibitory activity. Preincorporation of CD59 into these cells inhibited lysis by C5b-9, regardless of whether CD59 was added before or after assembly of the C5b-8 complex. When incorporated into the membrane, CD59 inhibited binding of 125I-C9 to membrane C5b-8 and reduced the extent of formation of SDS-resistant C9 polymer. The inhibitory effect of CD59 on 125I-C9 incorporation was most pronounced at near-saturating input of C9 (to C5b-8). By contrast, CD59 did not inhibit either C5b67 deposition onto the cell surface, or, binding of 125I-C8 to preassembled membrane C5b67. Taken together, these data suggest that CD59 exerts its C-inhibitory activity by limiting incorporation of multiple C9 into the membrane C5b-9 complex.
The Tetraspan Protein Epithelial Membrane Protein-2 Interacts with β1 Integrins and Regulates Adhesion (2002)

The growth arrest-specific-3 (GAS3)/PMP22 proteins are members of the four-transmembrane (tetraspan) superfamily. Although the function of these proteins is poorly understood, GAS3/PMP22 proteins have been implicated in the control of growth and progression of certain cancers. Epithelial membrane protein-2 (EMP2), a GAS3/PMP22 family member, was recently identified as a putative tumor suppressor gene. Here, we addressed the normal function of EMP2 by testing the prediction that it influences integrin-related cell functions. We observed that EMP2 associates with the beta(1) integrin subunit. Co-immunoprecipitation and immunodepletion experiments indicated that approximately 60% of beta(1) integrins and EMP2 can be isolated in common protein complexes. Whereas this association between EMP2 and beta(1) integrin may be direct or indirect, it has features of integrin heterodimer selectivity. Thus, by laser confocal microscopy, EMP2 colocalized with alpha(6)beta(1) but not alpha(5)beta(1) integrin. Increased expression of EMP2 also influenced the integrin heterodimer repertoire present on the plasma membrane. EMP2 specifically increased the surface expression of the alpha(6)beta(1) integrin while decreasing that of the alpha(5)beta(1) protein. Reciprocally, reduction in EMP2 expression using a specific ribozyme decreased surface expression of alpha(6)beta(1) integrin. Accordingly, these EMP2-mediated changes resulted in a dramatic alteration in cellular adhesion to extracellular matrix proteins. This study demonstrates for the first time the interaction of a GAS3/PMP22 family member with an integrin protein and suggests that such interactions and their functional consequences are a physiologic role of GAS3/PMP22 proteins.
SILICON ON INSULATOR TECHNOLOGY REVIEW (2011)

An effort to reduce the power consumption of the circuit, the supply voltage can be reduced leading to reduction of dynamic and static power consumption. This paper introduces one of the greatest future technologies of this decade and that is SOI technology. Silicon-On-Insulator transistors are fabricated in a small (~100 nm) layer of silicon, located on top of a silicon dioxide layer, called buried oxide. This oxide layer provides full dielectric isolation of the transistor and thus most of the parasitic effects present in bulk silicon transistors are eliminated. The structure of the SOI transistor is depicted and is very similar to that of the bulk transistor. The main difference is the presence of the buried oxide it provides attractive properties to the SOI transistor. Power has become one of the most important paradigms of design convergence for multi gigahertz communication such as optical data links wireless products and microprocessor ASIC/SOC designs. POWER consumption has become a bottleneck in microprocessor design. For more than three decades, scientists have been searching for a way to enhance existing silicon technology to speed up the computer performance. This new success in harnessing SOI technology will result in faster computer chips that also require less power a key requirement for extending the battery life of small, hand-held devices that will be pervasive in the future. SOI is a major breakthrough because it advances chip manufacturing one to two years ahead of conventional bulk silicon. The following provides a step-by-step look at the developments leading up to the development of SOI technology.
Crest factor reduction for carrier aggregated OFDM systems (2012)

To achieve higher data rate as specified in LTE-Advanced, support of wider Bandwidths are required than the existing 20 MHz system bands. Carrier Aggregation is the technique deployed in LTE-A system that can extends system bandwidth up to 100 MHz which also increases the downlink spectral efficiency. N-x-OFDMA will be used as the downlink transmission scheme in LTE-A. The primary concern in carrier aggregation is the significant increase in PAPR of the time domain signal, to accommodate large peaks PA has to operate with sufficient power back-off which results in lower operating efficiency. We propose two stages of Noise Shaping process for reducing the PAPR of the carrier aggregated composite signal which is a transmitter- side processing technique and does not require any information at the receiver. This paper analyzes different PAPR scenarios using contiguous & non-contiguous frequency bands for carrier aggregation and the simulation result shows the performance improvement in PAPR of about 3 & 4dB respectively.
A novel approach for preparing anti‐T cell receptor constant region antibodies (1986)

To obtain antibodies against the individual chains of the T cell receptor (TCR) complex, we have produced chimeric proteins containing domains from immunoglobulin (Ig) and TCR polypeptide chains. Basically, the Ig light chains were used as carriers for the TCR constant (C) region domains. The exons which encode the main body of the C regions of the alpha, beta and the related gamma polypeptide chains were "engineered" into the intronic region between the rearranged Ig variable (V) region and C kappa region genes. All three chimeric genes were expressed in myeloma cells, and the proteins of expected apparent molecular weight were produced. Secreted proteins containing the C beta domain were purified from the culture supernatant by using anti-kappa antibody affinity columns, and two rabbits were then immunized with the purified protein. Both rabbits produced antibodies able to immunoprecipitate the heterodimeric TCR protein.
Sort vs. Hash revisited (2009)

Join is an important database operation. As computer architectures evolve, the best join algorithm may change hand. This paper re-examines two popular join algorithms -- hash join and sort-merge join -- to determine if the latest computer architecture trends shift the tide that has favored hash join for many years. For a fair comparison, we implemented the most optimized parallel version of both algorithms on the latest Intel Core i7 platform. Both implementations scale well with the number of cores in the system and take advantages of latest processor features for performance. Our hash-based implementation achieves more than 100M tuples per second which is 17X faster than the best reported performance on CPUs and 8X faster than that reported for GPUs. Moreover, the performance of our hash join implementation is consistent over a wide range of input data sizes from 64K to 128M tuples and is not affected by data skew. We compare this implementation to our highly optimized sort-based implementation that achieves 47M to 80M tuples per second. We developed analytical models to study how both algorithms would scale with upcoming processor architecture trends. Our analysis projects that current architectural trends of wider SIMD, more cores, and smaller memory bandwidth per core imply better scalability potential for sort-merge join. Consequently, sort-merge join is likely to outperform hash join on upcoming chip multiprocessors. In summary, we offer multicore implementations of hash join and sort-merge join which consistently outperform all previously reported results. We further conclude that the tide that favors the hash join algorithm has not changed yet, but the change is just around the corner.
Increased erythritol production in fed-batch cultures of Torula sp. by controlling glucose concentration (2001)

The effect of glucose concentration on erythritol production by Torula sp. was investigated. The maximum volumetric productivity of erythritol was obtained at an initial glucose concentration of 300 g l(-1) in batch culture. The volumetric productivity was maximal at a controlled glucose concentration of 225 g l(-1), reducing the lag time of the erythritol production. A fed-batch culture was established with an initial glucose concentration of 300 g l(-1) and with a controlled glucose concentration of 225 g l(-1) in medium containing phytic acid as a phosphate source. In this fed-batch culture, a final erythritol production of 192 g l(-1) was obtained from 400 g l(-1) glucose in 88 h. This corresponded to a volumetric productivity of 2.26 g l(-1) h(-1) and a 48% yield.
Enzymic Grafting of Amylose from Poly(dimethylsiloxanes) (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnzymic Grafting of Amylose from Poly(dimethylsiloxanes)Volker v. Braunmuehl, Gerd Jonas, and Reimund StadlerCite this: Macromolecules 1995, 28, 1, 17–24Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://doi.org/10.1021/ma00105a003RIGHTS & PERMISSIONSArticle Views323Altmetric-Citations98LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (986 KB) Get e-Alerts Get e-Alerts
Relations between depression and cultural contingencies in modern societies: a behavioral analytic account (2011)

Na analise do comportamento, a depressao tem sido definida com a referencia a um conjunto de relacoes envolvendo variaveis ontogeneticas e culturais. Este trabalho salienta, a partir de uma interpretacao analitico-comportamental, variaveis culturais que definem como a depressao se configura em sociedades individualizadas. Apresenta-se, inicialmente, uma sintese da abordagem analitico-comportamental para a depressao. Em seguida, discute-se a depressao a luz de um modelo de complexidade, com base no qual e concebida em termos das relacoes que a constituem. Assinala-se que a participacao de relacoes determinadas por variaveis culturais confere maior complexidade ao fenomeno, refletindo sobre os tipos de intervencao necessarios. Finalmente, sao elencados aspectos centrais do processo de individualizacao, indicando-se como arranjos de contingencias especificas das sociedades modernas, individualizadas, promoveram modos especificos de relacao dos individuos consigo mesmos e com os outros, determinando a aquisicao e manutencao de certos repertorios comportamentais classificados como depressao.
Proteolytically cleaved MLL subunits are susceptible to distinct degradation pathways (2011)

The mixed lineage leukemia (MLL) proto-oncogenic protein is a histone-lysine N-methyltransferase that is produced by proteolytic cleavage and self-association of the respective functionally distinct subunits (MLL(N) and MLL(C)) to form a holocomplex involved in epigenetic transcriptional regulation. On the basis of studies in Drosophila it has been suggested that the separated subunits might also have distinct functions. In this study, we used a genetically engineered mouse line that lacked MLL(C) to show that the MLL(N)-MLL(C) holocomplex is responsible for MLL functions in various developmental processes. The stability of MLL(N) is dependent on its intramolecular interaction with MLL(C), which is mediated through the first and fourth plant homeodomain (PHD) fingers (PHD1 and PHD4) and the phenylalanine/tyrosine-rich (FYRN) domain of MLL(N). Free MLL(N) is destroyed by a mechanism that targets the FYRN domain, whereas free MLL(C) is exported to the cytoplasm and degraded by the proteasome. PHD1 is encoded by an alternatively spliced exon that is occasionally deleted in T-cell leukemia, and its absence produces an MLL mutant protein that is deficient for holocomplex formation. Therefore, this should be a loss-of-function mutant allele, suggesting that the known tumor suppression role of MLL may also apply to the T-cell lineage. Our data demonstrate that the dissociated MLL subunits are subjected to distinct degradation pathways and thus not likely to have separate functions unless the degradation mechanisms are inhibited.
Enhanced optical waveguide light mode spectroscopy via detection of fluorophore absorbance (2006)

A novel technique based on surface sensitive absorbance detection using an optical waveguide light mode spectroscopy (OWLS) instrument is presented. The proof of concept for this extension of a standard technique is demonstrated by painting an increasing number of ink lines on a waveguide, perpendicular to the light path, while monitoring the outcoupled light intensity. Furthermore, by the adsorption of poly(L-lysine)-graft-poly(ethylene glycol) as a model system with contents of 5%, 10%, 25%, and 50% labeled polymer, the in situ performance is demonstrated, and the absorbance signal is calibrated such that it can be converted into adsorbed mass. The simultaneous detection of labeled and label-free species allows for the study of complex experimental setups whereby monitoring of adsorption, desorption, and even exchange processes becomes possible. The sensitivity of the absorbance detection exceeds standard OWLS by one to two orders of magnitude.