gebhart/patrepeval huggingface dataset:
Vaccine adjuvants: Current state and future trends (2004)

The problem with pure recombinant or synthetic antigens used in modern day vaccines is that they are generally far less immunogenic than older style live or killed whole organism vaccines. This has created a major need for improved and more powerful adjuvants for use in these vaccines. With few exceptions, alum remains the sole adjuvant approved for human use in the majority of countries worldwide. Although alum is able to induce a good antibody (Th2) response, it has little capacity to stimulate cellular (Th1) immune responses which are so important for protection against many pathogens. In addition, alum has the potential to cause severe local and systemic side-effects including sterile abscesses, eosinophilia and myofascitis, although fortunately most of the more serious side-effects are relatively rare. There is also community concern regarding the possible role of aluminium in neurodegenerative diseases such as Alzheimer's disease. Consequently, there is a major unmet need for safer and more effective adjuvants suitable for human use. In particular, there is demand for safe and non-toxic adjuvants able to stimulate cellular (Th1) immunity. Other needs in light of new vaccine technologies are adjuvants suitable for use with mucosally-delivered vaccines, DNA vaccines, cancer and autoimmunity vaccines. Each of these areas are highly specialized with their own unique needs in respect of suitable adjuvant technology. This paper reviews the state of the art in the adjuvant field, explores future directions of adjuvant development and finally examines some of the impediments and barriers to development and registration of new human adjuvants.
YC‐1 attenuates LPS‐induced proinflammatory responses and activation of nuclear factor‐κB in microglia (2007)

YC-1 was able to inhibit LPS-induced iNOS and COX-2 expression and NF-kappaB activation, indicating that YC-1 may be developed as an anti-inflammatory neuroprotective agent.
Tetracycline-Controlled Gene Expression System Achieves High-Level and Quantitative Control of Gene Expression (1996)

The tetracycline-controlled gene expression system utilizes the control elements of the tetracycline resistance operon encoded in TnlO of Escherichia coli to control gene expression in eukaryotic cells. Here we demonstrate the quantitative control of the expression of the luciferase gene, dihydrofolate reductase gene, and bcl-2 gene in HeLa S3 or Chinese hamster ovary AA8 cells using the tetracycline-controlled gene expression system. Regardless of the host cell lines or the genes being expressed, there is a common range of tetracycline concentration within which the expression of genes is most sensitively regulated. In addition, the maximal gene expression level of the tetracycline-controlled gene expression system is higher than that of the wild-type CMV promoter/enhancer-driven system. Nonetheless, careful selection of stably transfected clones is necessary to achieve the optimally regulated gene expression using this system.
Roles of mitochondria in human disease (2010)

The chapters throughout this volume illustrate the many contributions of mitochondria to the maintenance of normal cell and tissue function, experienced as the health of the individual. Mitochondria are essential for maintaining aspects of physiology as fundamental as cellular energy balance, the modulation of calcium signalling, in defining cellular redox balance, and they house significant biosynthetic pathways. Mitochondrial numbers and volume within cells are regulated and have an impact on their functional roles, while, especially in the CNS (central nervous system), mitochondrial trafficking is critical to ensure the cellular distribution and strategic localization of mitochondria, presumably driven by local energy demand. Maintenance of a healthy mitochondrial population involves a complex system of quality control, involving degrading misfolded proteins, while damaged mitochondria are renewed by fusion or removed by autophagy. It seems evident that mechanisms that impair any of these processes will impair mitochondrial function and cell signalling pathways, leading to disordered cell function which manifests as disease. As gatekeepers of cell life and cell death, mitochondria regulate both apoptotic and necrotic cell death, and so at its most extreme, disturbances involving these pathways may trigger untimely cell death. Conversely, the lack of appropriate cell death can lead to inappropriate tissue growth and development of tumours, which are also characterized by altered mitochondrial metabolism. The centrality of mitochondrial dysfunction to a surprisingly wide range of major human diseases is slowly becoming recognized, bringing with it the prospect of novel therapeutic approaches to treat a multitude of unpleasant and pervasive diseases.
Antisense Inhibition of Rubisco Activase Increases Rubisco Content and Alters the Proportion of Rubisco Activase in Stroma and Thylakoids in Chloroplasts of Rice Leaves (2006)

From the changes in RCA distribution and alterations in Rubisco activity, RCA in the stroma of the chloroplast probably contributes to the activation of Rubisco, and RCA in thylakoids compensates for the reduction of RCA in the stroma, allowing steady-state photosynthesis to be maintained when RCA is depleted. RCA may also have a second role in protecting membranes against environmental stresses as a chaperone.
Effects of degree of fat saturation on fiber digestion and microbial protein synthesis when diets are fed twelve times daily. (2000)

Three Holstein heifers and one nonlactating cow, fitted with ruminal and duodenal cannulas, were arranged in a 4 × 4 Latin square design to determine the effects of degree of fat saturation on ruminal neutral detergent fiber digestion and microbial protein synthesis and to determine whether changes in the efficiency of microbial protein synthesis were related to protozoal populations in the rumen. Corn silage-based diets contained no added fat or 4.85% of diet dry matter as partially hydrogenated tallow, tallow, or animal-vegetable fat. Iodine values of fat sources were 12.8, 50.6, and 109.7 for partially hydrogenated tallow, tallow, and animal-vegetable fat, respectively. Cattle were fed every 2 h and consumed 1.5% of body weight as dry matter daily. Ruminal neutral detergent fiber digestibility was decreased by added fat but was not affected by increasing iodine value. Flows of microbial N and non-NH3-nonmicrobial N to the duodenum were not affected by treatment. Ruminal protozoa concentration decreased linearly as the iodine value of fats increased. The efficiency of microbial protein synthesis was increased and protozoa concentrations tended to decrease when fat was fed. Decreased ruminal protozoa concentration may have decreased intraruminal N recycling. Biohydrogenation of added fat may result in a low ruminal concentration of unsaturated fatty acids when cows are fed frequently, reducing the negative effects of unsaturated fat sources on ruminal neutral detergent fiber digestibility. Protozoa were inhibited by unsaturated fat, but it is not clear if biohydrogenation and frequent feeding lessened inhibition.
On parallelizability of stochastic gradient descent for speech DNNS (2014)

This paper compares the theoretical efficiency of model-parallel and data-parallel distributed stochastic gradient descent training of DNNs. For a typical Switchboard DNN with 46M parameters, the results are not pretty: With modern GPUs and interconnects, model parallelism is optimal with only 3 GPUs in a single server, while data parallelism with a minibatch size of 1024 does not even scale to 2 GPUs. We further show that data-parallel training efficiency can be improved by increasing the minibatch size (through a combination of AdaGrad and automatic adjustments of learning rate and minibatch size) and data compression. We arrive at an estimated possible end-to-end speed-up of 5 times or more. We do not address issues of robustness to process failure or other issues that might occur during training, nor of speed of convergence differences between ASGD and SGD parameter update patterns.
The extraction and purification of human pituitary follicle-stimulating hormone and luteinizing hormone. (1988)

Techniques are described for the extraction and purification of FSH and LH from human pituitary glands. The methodology uses acetone and alcohol for dehydration, extraction and precipitation of materials from both acetone preserved and fresh frozen glands. The processing involves the use of anion and cation exchange chromatography, adsorption techniques, and molecular sieve chromatography. Immunoaffinity extraction and purification of the gonadotropic hormones are also described. The specific activities of the LH preparations from acetone-preserved glands and from fresh frozen glands ranged from 11,200 to 12,640 IU/mg (68/40) and contained less than 0.2% TSH and FSH contamination. The yield varied from 9% to 18%. The specific activities of the FSH preparations from fresh frozen glands and from acetone preserved glands by affinity extraction (4990 and 4600 IU/mg 78/549) were higher than that from acetone preserved glands (3235 IU/mg). The LH and TSH contamination was less than 0.5%. The highest yield of FSH (34%) was obtained by affinity extraction. The preparations are of sufficient purity for use as immunogens and standards and for the preparation of radioligands to be used in specific and sensitive immunoassays.
Preferential Destruction of Metallic Single-Walled Carbon Nanotubes by Laser Irradiation (2006)

Upon laser irradiation in air, metallic single-walled carbon nanotubes (SWNTs) in carbon nanotube thin film can be destroyed in preference to their semiconducting counterparts when the wavelength and power intensity of the irradiation are appropriate and the carbon nanotubes are not heavily bundled. Our method takes advantage of these two species' different rates of photolysis-assisted oxidation, creating the possibility of defining the semiconducting portions of carbon nanotube (CNT) networks using optical lithography, particularly when constructing all-CNT FETs (without metal electrodes) in the future.
Noninvasive Estimation of Tissue Temperature Via High-Resolution Spectral Analysis Techniques (2005)

We address the noninvasive temperature estimation from pulse-echo radio frequency signals from standard diagnostic ultrasound imaging equipment. In particular, we investigate the use of a high-resolution spectral estimation method for tracking frequency shifts at two or more harmonic frequencies associated with temperature change. The new approach, employing generalized second-order statistics, is shown to produce superior frequency shift estimates when compared to conventional high-resolution spectral estimation methods Seip and Ebbini (1995). Furthermore, temperature estimates from the new algorithm are compared with results from the more commonly used echo shift method described in Simon et al. (1998).
Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase (1992)

In Escherichia coli, chorismate lyase catalyzes the first step in ubiquinone biosynthesis, the conversion of chorismate to 4-hydroxybenzoate. 4-Hydroxybenzoate is converted to 3-octaprenyl-4-hydroxybenzoate by 4-hydroxybenzoate octaprenyltransferase. These two enzymes are encoded by ubiC and ubiA, respectively, and have been reported to map near one another at 92 min on the E. coli chromosome. We have cloned the ubiCA gene cluster and determined the nucleotide sequence of ubiC and a portion of ubiA. The nucleotide sequence abuts with a previously determined sequence that encodes a large portion of ubiA. ubiC was localized by subcloning, and overproducing plasmids were constructed. Overexpression of ubiC allowed the purification of chorismate lyase to homogeneity, and N-terminal sequence analysis of chorismate lyase unambiguously defined the beginning of the ubiC coding region. Although chorismate lyase showed no significant amino acid sequence similarity to 4-amino-4-deoxychorismate lyase (4-amino-4-deoxychroismate----4-aminobenzoate), the product of E. coli pabC, chorismate lyase overproduction could complement the growth requirement for 4-aminobenzoate of a pabC mutant strain. Of the several enzymes that convert chorismate to intermediates of E. coli biosynthetic pathways, chorismate lyase is the last to be isolated and characterized.
Vapor-Liquid Equilibria of Formaldehyde-Methanol-Water (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVapor-Liquid Equilibria of Formaldehyde-Methanol-WaterStanley J. Green and Raymond E. VenerCite this: Ind. Eng. Chem. 1955, 47, 1, 103–109Publication Date (Print):January 1, 1955Publication History Published online1 May 2002Published inissue 1 January 1955https://pubs.acs.org/doi/10.1021/ie50541a037https://doi.org/10.1021/ie50541a037research-articleACS PublicationsRequest reuse permissionsArticle Views1248Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Amphiphilic Elastin-Like Block Co-Recombinamers Containing Leucine Zippers: Cooperative Interplay between Both Domains Results in Injectable and Stable Hydrogels (2015)

Many biological processes are regulated by reversible binding events, with these interactions between macromolecules representing the core of dynamic chemistry. As such, any attempt to gain a better understanding of such interactions, which would pave the way to the extrapolation of natural designs to create new advanced systems, is clearly of interest. This work focuses on the development of a leucine zipper-elastin-like recombinamer (ZELR) in order to elucidate the behavior of such domains when coexisting along the same molecule and to engineer reversible, injectable and stable hydrogels. The unique propensity of the Z-moiety selected to dimerize, together with the thermosensitive behavior of the ELR, which has been constructed as a thermosensitive amphiphilic tetrablock, has been engineered into a single recombinant molecule. In this molecular design, the Z-moieties are unable to form a network, while the ELR is below its Tt, thus, guaranteeing the liquid-like state of the system. However, this situation changes rapidly as the temperature increases above Tt, where a stable hydrogel is formed, as demostrated by rheological tests. The inability of the ELR molecule (without Z-domains) to form such a stable hydrogel above Tt clearly points to a positive cooperative effect between these two domains (Z and EL), and no conformational changes in the former are involved, as demonstrated by circular dichroism analysis. AFM shows that Z-motifs seem to induce the aggregation of micelles, which supports the enhanced stability displayed by ZELRs when compared to ELR at the macroscale level. To the best of our knowledge, this is the first time that such an interplay between these two domains has been reported. Furthermore, the cytocompatibility of the resulting hydrogels opens the door to their use in biomedical applications.
Enzyme immunoconjugates utilizing glucose oxidase and myeloperoxidase are cytotoxic to Candida tropicalis (1990)

A dual-enzyme immunoconjugate system was evaluated for its cytotoxic effect on Candida tropicalis. Glucose oxidase, which generates hydrogen peroxide in the presence of glucose and oxygen and myeloperoxidase, which catalyzes the oxidation of halides in the presence of hydrogen peroxide, were each conjugated to a C. tropicalis-specific monoclonal antibody. Neither the glucose oxidase nor the myeloperoxidase conjugates exhibited any significant cytotoxic effect by themselves. A combination of glucose oxidase conjugate (3.2 ng/ml) and myeloperoxidase conjugate (12.8 ng/ml) in the presence of 5 mg of glucose per ml, 150 mM chloride, and 50 microM iodide was cytotoxic to C. tropicalis, killing 99.9% of the treated sample. Flow cytometry was used to characterize the binding of the conjugates to yeast cells and demonstrated that the binding of both conjugates to the yeast cell surface is required for cytotoxicity. In addition, the concentrations of conjugates required for a cytotoxic effect were below the concentrations required to saturate all of the yeast cell surface antibody-binding sites.
Reading DNA differently (1998)

Product inhibition has provided the limiting barrier to efficient template-directed ligation and polymerization reactions. Here we review the attempts to circumvent this limitation and outline a translation strategy that does overcome the barrier and allows the information encoded in DNA to be read and amplified into backbone-modified oligonucleotides.
A flash-memory based file system (1995)

A flash memory device driver that supports a conventional UNIX file system transparently was designed. To avoid the limitations due to flash memory&amp;apos;s restricted number of write cycles and its inability to be overwritten, this driver writes data to the flash memory system sequentially as a Log-structured File System (LFS) does and uses a cleaner to collect valid data blocks and reclaim invalid ones by erasing the corresponding flash memory regions. Measurements showed that the overhead of the cleaner has little effect on the performance of the prototype when utilization is low but that the effect becomes critical as the utilization gets higher, reducing the random write throughput from 222 Kbytes/s at 30% utilization to 40 Kbytes/s at 90% utilization. The performance of the prototype in the Andrew Benchmark test is roughly equivalent to that of the 4.4BSD Pageable Memory based File System (MFS). 1. Introduction Flash memory, a nonvolatile memory IC (Integrated Circuit) that can hold d...
High-radiance room-temperature GaAs laser with controlled radiation in a single transverse mode (1972)

A GaAs laser for room-temperature operation is reported with high radiance and short delay times. For high radiance, the radiation of a 200-μ-wide GaAs laser diode is confined to the lowest order transverse mode. This is accomplished by operating the laser diode with an optical cavity in which the transverse mode is controlled by aperture-limiting slits. In the cavity, built of spherical lenses and plane mirrors, the mode width and height coincide with the width and height of the active region of the injection laser. The diffraction losses of the higher order transverse modes introduced by the apertures are sufficiently high to yield controlled radiation in the TEM <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">00</inf> mode ; this control was not always assured in the earlier design of the GaAs laser with the external resonator [1]. For short delays of the onset of stimulated emission, GaAs lasers are used for which the transition from short to long delays occurs above room temperature. Also, the external cavity is coupled closer to the active region of the semiconductor diode than reported previously [1] to minimize losses that would increase the delay times. Test results on the radiance, room-temperature delay times, and mode structure of the GaAs laser with the optical cavity are presented and the observed spiking pattern is discussed.
Characterisation of Bifidobacterium animalis subsp. lactis BB-12 and other probiotic bacteria using genomics, transcriptomics and proteomics (2005)

Determination of the complete genome sequence of a probiotic bacterium allows a number of new approaches to understanding the biology of the bacterium and provides new insight into the beneficial effects obtained through the use of probiotic products. Genomic information for B. animalis subsp. lactis strain BB-12 has been used to make microarrays and characterise the proteome of this important probiotic strain. These tools have been used to characterise other Bifidobacterium strains and to develop a better understanding of the stresses BB-12 is exposed to during industrial-scale production. In addition, changes in gene expression when cells are grown in the presence of bile salts or fructooligosaccharides have been determined. A comparison of information derived from the genome sequence of BB-12 to information derived from the published genome sequences of other probiotic bacteria is also provided.
Nodular lymphocyte-predominant Hodgkin lymphoma with nodules resembling T-cell/histiocyte-rich B-cell lymphoma: differential diagnosis between nodular lymphocyte-predominant Hodgkin lymphoma and T-cell/histiocyte-rich B-cell lymphoma (2003)

Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) and T-cell/histiocyte-rich B-cell lymphoma (T/HRBCL) are distinct tumors and are treated differently. They are linked by a morphologic and probably a biologic continuum, which renders the differential diagnosis difficult. To develop criteria to distinguish the entities along the morphologic continuum, we correlated the lymph node architecture and immunophenotype of both tumor cells and reactive components of 235 neoplasms in the spectrum of NLPHL and T/HRBCL with clinical data. Two hundred and eighteen cases fitted the World Health Organization (WHO) criteria of NLPHL (139) or T/HRBCL (79). While tumor cells in both entities were immunophenotypically similar, background composition differed: in NLPHL small B cells and CD3+CD4+CD57+ T cells were common, whereas in T/HRBCL, CD8+ cytotoxic T cells and histiocytes dominated. Follicular dendritic cells (FDCs) formed expanded meshworks in NLPHL, whereas they were absent in T/HRBCL. Seventeen cases represented a gray zone: within FDC meshworks, neoplastic B cells resided in a background depleted of small B cells but rich in T cells and histiocytes. Tumor cells either were loosely scattered or formed clusters, thus resembling areas of either T/HRBCL or inflammatory diffuse large BCL (DLBCL) within the nodules. Patients with these NLPHLs with T-cell/histiocyte-rich nodules presented at a high stage and with B symptoms, as in T/HRBCL, but had an excellent survival, as in NLPHL. This morphologic pattern suggests a biologic continuum between NLPHL and T/HRBCL.
Growth of strained Si on high-quality relaxed Si1−xGex with an intermediate Si1−yCy layer (2005)

An intermediate Si1−yCy layer in the Si1−xGex film, replacing the conventionally graded buffer layer, was used to form the high-quality relaxed SiGe substrate. With the 700 nm thick Si0.8Ge0.2 overlayer, such a Si1−xGex∕Si1−yCy∕Si1−xGex (x=0.2, y−0.014) heterostructure has a threading dislocation density of 5.5×105cm−2 and a residual strain of only 2%. The surface roughness was measured to be about 4.2 nm. The long-range misfit dislocation array was formed mainly at the interface of top Si1−xGex and Si1−yCy. Strained-Si n-channel metal-oxide-semiconductor transistors with various buffer layers were fabricated and examined. Effective electron mobility for the strained-Si device with this substrate technology was found to be 95% higher than that of Si control device. The scheme for the formation of the relaxed Si1−xGex film serving as a virtual substrate shall be applicable to high-speed strained-Si devices.