gebhart/patrepeval huggingface dataset:
Induction of Phytochelatin and Variation of Glutathione Level in Fruits and Callus Cultures of Pumpkin by Treatment with Heavy Metals (1990)

Three kinds of phytochelatins, (γ-Glu-Cys)2-Gly [PC2], (γ-Glu-Cys)3-Gly [PC3], and (γ-Glu-Cys)4-Gly [PC4], were synthesized in callus induced from parenchymatous tissues of pumpkin fruit when it was transplanted to liquid medium containing 40μM CdSO4. After transplantation, PC2 and PC3 were found within 1 day and PC4 was found in several days. Glutathione level in the callus cultures rapidly decreased to 30-40% of the initial level, though glutathione appeared to be newly produced during synthesis of phytochelatin. Addition of 2mM glutathione to the Cd-containing medium increased glutathione and phytochelatin levels in the pumpkin callus cultures, suggesting that glutathione level might be one of the factors controlling the maximum amount of phytochelatin synthesis in the callus cultures. Phytochelatin synthesis was induced by Cd, Ag and Hg. High concentrations of Ni and Zn were also able to induce it. On the other hand, accumulation of the three phytochelatins and the decrease of glutathione level were also observed in the parenchymatous tissues of pumpkin fruit treated with 15μM Cd for 4 days.
Fully automated liquid extraction‐based surface sampling and ionization using a chip‐based robotic nanoelectrospray platform (2009)

A fully automated liquid extraction-based surface sampling device utilizing an Advion NanoMate chip-based infusion nanoelectrospray ionization system is reported. Analyses were enabled for discrete spot sampling by using the Advanced User Interface of the current commercial control software. This software interface provided the parameter control necessary for the NanoMate robotic pipettor to both form and withdraw a liquid microjunction for sampling from a surface. The system was tested with three types of analytically important sample surface types, viz., spotted sample arrays on a MALDI plate, dried blood spots on paper, and whole-body thin tissue sections from drug dosed mice. The qualitative and quantitative data were consistent with previous studies employing other liquid extraction-based surface sampling techniques.
Virus Levels in Untreated African Infants Infected with Human Immunodeficiency Virus Type 1 (1999)

In developed areas, human immunodeficiency virus (HIV)-infected infants have high virus levels and rapidly progress to death. HIV levels were assessed in 1994-1997 in untreated infants in Malawi by analysis of dried blood spots tested by nucleic acid silica-bound amplification. Of 24 umbilical cord blood (CB)-positive samples, 83% had >10,000 copies/mL. The median virus level was 78,000 copies/mL. First positive sample median levels were 355,000 copies/mL among 52 perinatally infected infants and 130,000 copies/mL among 43 infants infected by breast-feeding. Virus levels were stable, and initial levels predicted levels 1 year after infection (P=.005), at which time levels did not significantly differ among in utero, perinatally, or postnatally infected infants. Thus, neither age at infection nor route of infection significantly influenced HIV levels measured 1 year after infection. Most (87%) CB-positive infants were infected before labor onset, since virus levels greatly exceeded those expected in their mothers.
Damage to Lipids, Proteins, DNA, and RNA in Mild Cognitive Impairment (2007)

Free radical-mediated oxidative damage is thought to play a role in the pathogenesis of Alzheimer disease. Previous studies have shown oxidative damage to lipids, proteins, DNA, and RNA in multiple brain regions in late-stage Alzheimer disease. Recent studies on patients with amnestic mild cognitive impairment who have undergone autopsy have shown increased lipid peroxidation as well as protein, DNA, and RNA oxidation in multiple brain regions. These studies establish oxidative damage as an early event in the pathogenesis of Alzheimer disease that can serve as a therapeutic target to slow the progression or perhaps the onset of the disease.
Epitaxial growth of high-quality Ge films on nanostructured silicon substrates (2006)

Low (∼105cm−2) defect density, thick (∼10μm) epitaxially grown Ge films on nanostructured and planar silicon substrates using chemical vapor deposition are reported. The structural morphology of Ge films was evaluated using scanning electron microscopy, transmission electron microscopy, and high resolution x-ray diffraction measurements. The surface defect density was measured by counting etch pits. The growth quality of Ge films was significantly superior on nanostructured surfaces relative to the planar as demonstrated by (a) reduction in defect density from ∼6×108cm−2 for planar to ∼5×105cm−2, (b) reduction in Δω full width half maximum peaks of the reciprocal space maps of Ge epilayers from 373arcsec on planar (unpatterned) to 93arcsec on the nanostructured surfaces, and (c) the elimination of crosshatch pattern characteristic of planar surface growth.
Non-Orthogonal Multiple Access (NOMA) for Cellular Future Radio Access (2013)

This paper presents a non-orthogonal multiple access (NOMA) concept for cellular future radio access (FRA) towards the 2020s information society. Different from the current LTE radio access scheme (until Release 11), NOMA superposes multiple users in the power domain although its basic signal waveform could be based on the orthogonal frequency division multiple access (OFDMA) or the discrete Fourier transform (DFT)-spread OFDM the same as LTE baseline. In our concept, NOMA adopts a successive interference cancellation (SIC) receiver as the baseline receiver scheme for robust multiple access, considering the expected evolution of device processing capabilities in the future. Based on system-level evaluations, we show that the downlink NOMA with SIC improves both the capacity and cell-edge user throughput performance irrespective of the availability of the frequency-selective channel quality indicator (CQI) on the base station side. Furthermore, we discuss possible extensions of NOMA by jointly applying multi-antenna/site technologies with a proposed NOMA/MIMO scheme using SIC and an interference rejection combining (IRC) receiver to achieve further capacity gains, e.g., a three-fold gain in the spectrum efficiency representing a challenging target for FRA.
Down‐regulation of BRCA1 and BRCA2 in human ovarian cancer cells exposed to adriamycin and ultraviolet radiation (1998)

Germ-line mutations of the BRCA1 and BRCA2 genes pre-dispose women to develop cancers of the breast and ovary, but the biologic functions of these genes remains unclear. We have investigated the responses of the BRCA1 and BRCA2 gene products to cytotoxic agents in 3 human ovarian cancer cell lines: SK-OV-3 (which contains a p53 deletion mutation), CAOV-3 (which over-expresses a mutant p53) and PA-1 (which expresses wild-type p53). In screening studies, we determined the effects of 7 different agents on BRCA1 and BRCA2 expression. We found that Adriamycin (ADR) and ultraviolet (UV)radiation significantly down-regulated BRCA1 and BRCA2 mRNA expression in SK-OV-3 cells. On the other hand, camptothecin, nitrogen mustard, taxol, vincristine and etoposide had no effect on BRCA1 or BRCA2 mRNA levels at doses that yielded degrees of cytotoxicity similar to or greater than ADR. The down-regulation of BRCA1 and BRCA2 mRNAs was dose and time dependent; significant down-regulation was first observed at 8–16 hr after exposure to ADR. BRCA1 protein levels were also down-regulated following treatment of SK-OV-3 cells with ADR. Similar results were observed in CAOV-3 and PA-1 cells treated with ADR, and this finding could not be directly attributed to ADR-induced changes in the cell cycle distribution. The ADR doses required for significant decreases of BRCA1 and BRCA2 were about 10–15, 5–10 and 2 μM, respectively, for SK-OV-3, CAOV-3 and PA-1; the IC50 doses for loss of cell viability (determined by Trypan blue dye exclusion) were 23, 14 and 0.4 μM, respectively. Thus, at equitoxic doses of ADR, PA-1 cells were more resistant to down-regulation of BRCA1 and BRCA2 than SK-OV-3 or CAOV-3. Our findings suggest that 1) BRCA1 and BRCA2 expression in human ovarian cancer cell lines is selectively down-regulated by 2 DNA-damaging agents (ADR and UV radiation); 2) these responses are not due to non-specific cytotoxicity; and 3) the BRCA1 and BRCA2 responses may be dependent, in part, on the p53 functional status of the cells. We speculate that the down-regulation of BRCA1 and BRCA2 may be part of a cellular survival response activated by certain forms of DNA damage. Int. J. Cancer 77:600–609, 1998. © 1998 Wiley-Liss, Inc.
Incorporation of nonnatural amino acids into proteins by using five-base codon-anticodon pairs (2000)

A novel strategy for the incorporation of nonnatural amino acids into proteins was developed by using five-base codon-anticodon pairs. The streptavidin mRNA containing five-base codon CGGUA and the chemically aminoacylated tRNA with five-base anticodon UACCG were prepared, and added into E. coli in vitro translation system. As a result, the nonnatural amino acid was successfully incorporated into desired position of the protein. Other five-base codons CGGN1N2, where N1 and N2 indicate one of four nucleotides, were also available for the incorporation of the nonnatural amino acid.
Aggregated α‐synuclein activates microglia: a process leading to disease progression in Parkinson's disease (2005)

A growing body of evidence indicates that an inflammatory process in the substantia nigra, characterized by activation of resident microglia, likely either initiates or aggravates nigral neurodegeneration in Parkinson's disease (PD). To study the mechanisms by which nigral microglia are activated in PD, the potential role of alpha-synuclein (a major component of Lewy bodies that can cause neurodegeneration when aggregated) in microglial activation was investigated. The results demonstrated that in a primary mesencephalic neuron-glia culture system, extracellular aggregated human alpha-synuclein indeed activated microglia; microglial activation enhanced dopaminergic neurodegeneration induced by aggregated alpha-synuclein. Furthermore, microglial enhancement of alpha-synuclein-mediated neurotoxicity depended on phagocytosis of alpha-synuclein and activation of NADPH oxidase with production of reactive oxygen species. These results suggest that nigral neuronal damage, regardless of etiology, may release aggregated alpha-synuclein into substantia nigra, which activates microglia with production of proinflammatory mediators, thereby leading to persistent and progressive nigral neurodegeneration in PD. Finally, NADPH oxidase could be an ideal target for potential pharmaceutical intervention, given that it plays a critical role in alpha-synuclein-mediated microglial activation and associated neurotoxicity.
Tachykinin NK3 and NK1 receptor activation elicits secretion from porcine airway submucosal glands (2003)

1 We presently characterized the tachykinin receptor subtypes, using tachykinin receptor agonists and selective antagonists, that induce submucosal gland fluid flux (J(G)) from porcine tracheal explants with the hillocks technique. We also investigated the effects of the tachykinin receptor agonists on the electrophysiologic parameters of the tracheal epithelium in Ussing chambers. 2 The NK(1) tachykinin receptor agonist substance P (SP, 1 microM) and the NK(3) tachykinin receptor agonist [MePhe(7)]neurokinin B ([MePhe(7)]NKB, 1 microM) induced gland fluid fluxes of 0.29+/-0.03 microl min(-1) cm(-2) (n=26) and 0.36+/-0.05 microl min(-1) cm(-2) (n=24), respectively; while the NK(2) tachykinin receptor agonist [betaAla(8)]neurokinin A (4-10) ([betaAla(8)]NKA (4-10), 1 microM) had no effect on J(G) (n=10). 3 The NK(1) receptor antagonist CP99994 (1 microM, n=9) blocked 93% of the SP-induced J(G), whereas the NK(3) receptor antagonist SB223412 (1 microM, n=12) had no effect on the SP-induced J(G). However, SB223412 (1 microM, n=9) blocked 89% of the [MePhe(7)]NKB-induced J(G) while CP99994 (1 microM, n=10) did not affect the [MePhe(7)]NKB-induced J(G). The NK(2) receptor antagonist SR48968 (1 microM) did not block the J(G) induced by either the NK(1) (n=4) or NK(3) (n=13) receptor agonists. 4 The nicotinic ganglionic acetylcholine receptor antagonist hexamethonium (1 microM) and the muscarinic acetylcholine receptor antagonist atropine (1 microM) also decreased the NK(3) receptor agonist-induced J(G) by 67% (n=10) and 71% (n=12), respectively. 5 The potential difference (PD), short-circuit current (I(SC)), and membrane resistance (R(M)) of the porcine tracheal epithelial membranes were not significantly affected by any of the neurokinin agonists or antagonists (1 microM, basolateral) used in this study, although SP and [betaAla(8)]NKA (4-10) induced a slight transient epithelial hyperpolarization. 6 These data suggest that NK(1) and NK(3) receptors induce porcine airway gland secretion by different mechanisms and that the NK(3) receptor agonists induced secretion is likely due to activation of prejunctional NK(3) receptors on parasympathetic nerves, resulting in acetylcholine-release. We conclude that tachykinin receptor antagonists may have therapeutic potential in diseases with pathophysiological mucus hypersecretion such as asthma and chronic bronchitis.
Surface Protein IsdC and Sortase B Are Required for Heme-Iron Scavenging ofBacillus anthracis (2006)

Bacillus anthracis, the spore-forming agent of anthrax, requires iron for growth and is capable of scavenging heme-iron during infection. We show here that the B. anthracis iron-regulated surface determinants (isd) locus encompasses isdC, specifying a heme-iron binding surface protein. Anchoring of IsdC to the cell wall envelopes of vegetative bacilli requires srtB, which encodes sortase B. Purified sortase B cleaves IsdC between the threonine and the glycine of its NPKTG motif sorting signal. B. anthracis variants lacking either isdC or srtB display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme.
[An experimental study on cultivation of human trigeminal ganglionic cell in vitro and its sensitivity to infection of herpes simplex virus type 1]. (1994)

We firstly report an experimental study on the sensitivity of primary cultural human trigeminal ganglionic cells infected by herpes simplex virus type 1 (HSV-1) in different time-points. The results indicate that the neuron of human trigeminal ganglia is the most sensitive cell type, the antigen of HSV-1 replicates rapidly within the cytoplasm and cell nucleus, the fibroblasts and glia cells are infected partly by the virus. Therefore, perhaps the neuron is the main harboring cell type.
Anatomy of the 5q- deletion: different sex ratios and deleted 5q bands in MDS and AML. (1996)

Deletion of part of the long arm of chromosome 5 (5q-) is the most common structural chromosome aberration in patients with myeloid disorders. Most patients are women. Although widely varying 5q segments are deleted, the vast majority of cases share a common deleted region (5q31). Whether or not other deleted 5q regions are of clinical importance is unknown. In an attempt to throw light on this question, 439 published 5q- cases were analyzed. The female preponderance was found to be limited to refractory anemia (RA), RA with excess of blasts (RAEB) and myeloproliferative disease, all with 5q- as the only chromosome aberration. When other chromosome aberrations had developed or the diagnosis was AML, only marginal female preponderance was observed. The 5q31 was deleted in all patients with MDS and 5q- as the only aberration. In contrast, in AML and following cytogenetic progression other bands were deleted more often than 5q31. This difference, together with a different female preponderance suggests diverse pathogenetic mechanisms in MDS and AML. Furthermore, the bands 5q13-5q21 were found to be of importance for clinical progression from RA to RAEB, and 5q22-5q33 of importance for development of AML and cytogenetic progression. In conclusion, the 5q bands deleted affect both clinical and cytogenetic progression.
Dark chocolate improves endothelial and platelet function: Figure 1 (2005)

<b>Background/aims:</b> Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. The purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes. <b>Methods:</b> Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry. <b>Results:</b> Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02). <b>Conclusion:</b> Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes.
Presiones políticas y comerciales (1997)

A lo largo de gran parte de la historia de Estados Unidos, los intereses comerciales han desempanado un rol central en la politica exterior y viceversa. En una era de companias con produccion en diversos paises o multiples alianzas estrategicas con firmas de otras naciones, los intereses de Washington y los del sector de negocios discrepan en mas de un aspecto. Durante las proximas decadas, la interaccion entre ellos se volvera mas intensa, mas importante, mas dificil de manejar y mas complicada.
The expression of the urokinase plasminogen activator system in metastatic murine osteosarcoma: an in vivo mouse model. (2001)

The role of urokinase plasminogen activator (uPA) in osteosarcoma is poorly understood. We examined the importance of uPA, its receptor, uPAR, and its inhibitor, PAI-1, in our in vivo model of metastatic osteosarcoma. Rodent osteosarcoma cells (UMR 106-01) were inoculated into the tibia of athymic mice. Animals were sacrificed and autopsied at 4 days to 5 weeks after inoculation. Tibiae and lungs were excised, fixed, and examined histologically and by in situ hybridization. Osteosarcoma development was associated with tibial swelling and lameness, and radiographic changes included osteolysis and new bone formation. Lung metastases developed spontaneously. In the tibial tumors, uPAR mRNA was expressed early (4 days), whereas uPA and PAI-1 mRNA increased as the tumor invaded the surrounding tissue (3 weeks). There was also an increase in the mRNA expression of the osteoblast-related genes, alpha1(I) procollagen and osteopontin, but not matrix Gla protein. Lung metastases also expressed mRNA for the uPA system and the bone-related proteins. We have produced a model of metastatic osteosarcoma, which typifies the characteristics of the human tumor. Our results suggest that the uPA system plays a role in the local aggressiveness and metastasis of osteosarcoma and, in particular, indicates a possible therapeutic role for uPAR antagonists in the treatment of osteosarcoma.
Effect of Nitrogen Mustard on Virus of Serum Hepatitis in Whole Blood. (1952)

1. Nitrogen mustard in a concentration of 500 mg per 1 failed to inactivate the Fort Bragg strain of serum hepatitis virus in whole blood. 2. Possible reasons for this failure are discussed. 3. The virus titer of the whole blood used was found to be at least 25000 infectious units/ml.
Molecular basis of the neuronal ceroid lipofuscinoses: Mutations inCLN1,CLN2,CLN3, andCLN5 (1999)

The neuronal ceroid lipofuscinoses (NCLs), also referred to as Batten disease, are a group of neurodegenerative disorders characterised by the accumulation of an autofluorescent lipopigment in many cell types. Different NCL types are distinguished according to age of onset, clinical phenotype, ultrastructural characterisation of the storage material, and chromosomal location of the disease gene. At least eight genes underlie the NCLs, of which four have been isolated and mutations characterised: CLN1, CLN2, CLN3, CLN5. Two of these genes encode lysosomal enzymes, and two encode transmembrane proteins, at least one of which is likely to be in the lysosomal membrane. The basic defect in the NCLs appears to be associated with lysosomal function.
Real-time PCR for universal antibiotic susceptibility testing (2004)

These results demonstrate that molecular detection of bacteria could be more rapid than phenotypic methods for antibiotic susceptibility testing.
NETPLAN-a telecommunications network planning support system (2003)

A software system, NETPLAN, for supporting telecommunications network planning has been developed. It is implemented using Prolog with a user-friendly graphical interface based on X Windows and Motif. The three basic activities of network planning (routing design, one-way and both-way junction circuit design, and signaling network design for circuit-switched networks) are fully supported. The dimensioning of the junction circuits is optimized by considering noncoincidental busy hour traffic characteristics of the various routes using the 24-hour traffic profile. The object-oriented approach adopted by NETPLAN eliminates the need for defining traffic and routing matrices as required by conventional approaches. The usefulness of the system is illustrated by its application to the planning of a network with ten switching nodes and twenty interconnected links.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Tailoring Longitudinal Surface Plasmon Wavelengths, Scattering and Absorption Cross Sections of Gold Nanorods (2008)

Tailoring the longitudinal surface plasmon wavelengths (LSPWs), scattering, and absorption cross sections of gold nanorods has been demonstrated by combining anisotropic shortening and transverse overgrowth and judiciously choosing starting Au nanorods. Shortening yields Au nanorods with decreasing lengths but a fixed diameter, while overgrowth produces nanorods with increasing diameters but a nearly unchanged length. Two series of Au nanorods with LSPWs varying in the same spectral range but distinct extinction coefficients are thus obtained. The systematic changes in the LSPW and extinction for the two series of Au nanorods are found to be in good agreement with those obtained from Gans theory. Dark-field imaging performed on two representative nanorod samples with similar LSPWs shows that the scattering intensities of the overgrown nanorods are much larger than those of the shortened nanorods. The experimental results are found to be in very good agreement with those obtained from finite-difference time-domain (FDTD) calculations. FDTD calculations further reveal that the scattering-to-extinction ratio increases linearly as a function of the diameter for Au nanorods with a fixed aspect ratio.