gebhart/patrepeval huggingface dataset:
Highly Efficient Synthesis of Low Polydispersity Core Cross‐Linked Star Polymers by Ru‐Catalyzed Living Radical Polymerization (2011)

The efficient formation of low polydispersity core cross-linked star (CCS) polymers via controlled/living radical polymerization (LRP) and the arm-first approach was found to be dependent on the mediating catalyst system. The Ru catalyst, Ru(Ind)Cl(PPh₃)₂ Cat. 1, and tertiary amine co-catalyst were used to synthesize highly living poly(methyl methacrylate) (PMMA) macroinitiators, which were then linked together with ethylene glycol dimethacrylate (EGDMA) to form PMMA(arm)PEGDMA(core) CCS polymers. The quantitative and near-quantitative synthesis of CCS polymers were observed for low to moderate molecular weight macroinitiators (M(n) = 8 and 20 kDa), respectively. Lower conversions were observed for high-molecular weight macroinitiators (M(n) ≥ 60 kDa). Overall, an improvement of between 10 and 20% was observed when comparing the Cat. 1 system to a conventional Cu-catalyzed system. This significant improvement in macroinitiator-to-star conversion is explained in the context of catalyst system selection and CCS polymer formation.
Synthesis and HIV‐1 Reverse Transcriptase Inhibitory Activity of Non‐Nucleoside Phthalimide Derivatives (2008)

Abstract A new type of non‐nucleoside HIV‐1 reverse transcriptase inhibitors in phthalimide series has been synthesized from either the reaction of N ‐carboethoxyphthalimide with amines or phthalimide with appropriate alkyl halides. The in vitro inhibitory activity of the synthesized compounds was studied by a radiometric assay at a concentration of 200 µg/mL using poly(rA)·oligo(dT) as a template‐primer and methyl‐[ 3 H]dTTP as a substrate. The three most potent compounds, N ‐( m , p ‐dihydroxybenzyl)phthalimide ( 11 ), N ‐[2‐( α ‐furyl)ethyl]phthalimide ( 29 ) and N ‐(5‐methylpyrazin‐2‐ylmethyl)phthalimide ( 25 ) exhibited IC 50 values of 60.90, 98.10 and 120.75 µg/mL, respectively, lower than IC 50 of delavirdine (502.22 µg/mL, using poly(rA)·oligo(dT) as a template‐primer and [ 3 H]dTTP as a substrate).
Imaging brain tumor by dendrimer-based optical/paramagnetic nanoprobe across the blood-brain barrier (2011)

A multimodal optical/paramagnetic nanoprobe, Den-Angio, was developed and demonstrated a capability to circumvent the blood brain barrier (BBB) and visualize brain tumors with high sensitivity in vivo. Den-Angio holds promise to pre-operatively localize brain tumors and make image-guided tumor resection possible during surgery.
p53-Dependent Regulation of Cdc6 Protein Stability Controls Cellular Proliferation (2005)

Activation of tumor suppressor p53 in response to genotoxic stress imposes cellular growth arrest or apoptosis. We identified Cdc6, a licensing factor of the prereplication complex, as a novel target of the p53 pathway. We show that activation of p53 by DNA damage results in enhanced Cdc6 destruction by the anaphase-promoting complex. This destruction is triggered by inhibition of CDK2-mediated CDC6 phosphorylation at serine 54. Conversely, suppression of p53 expression results in stabilization of Cdc6. We demonstrate that loss of p53 results in more replicating cells, an effect that can be reversed by reducing Cdc6 protein levels. Collectively, our data suggest that initiation of DNA replication is regulated by p53 through Cdc6 protein stability.
Synthesis of Open‐Chain 2,3‐Disubstituted 4‐nitroketones by Diastereoselective Michael‐addition of (E)‐Enamines to (E)‐Nitroolefins. A topological rule for C, C‐bond forming processes between prochiral centres. Preliminary communication (1981)

Abstract The Michael ‐additions of aliphatic, alicyclic, and arylsubstituted nitroolefins and enamines lead to γ‐nitroketones 3 in good chemical and excellent (&gt; 90%) diastereomeric yields (see Table 1 ). The known threo ‐configuration of one type of adducts 3 (entries 8, 10, and 11 of Table 1 ) can be arrived at by assuming the approach 8 of the Michael ‐acceptor and ‐donor; the reaction follows a topological rule , which is formulated and which is applicable to such diverse reactions as the diene synthesis, cyclopropanations, carbonyl olefinations and methylenations, aldol‐ and nitroaldol‐type additions, as well as additions of lithium, boron, and chromium derivatives to aldehydes (see 9 , 10 , 11 , and Table 2 ).
Real-Time Monocular SLAM with Straight Lines (2006)

The use of line features in real-time visual tracking applications is commonplace when a prior map is available, but building the map while tracking in real-time is much more difficult. We describe how straight lines can be added to a monocular Extended Kalman Filter Simultaneous Mapping and Localisation (EKF SLAM) system in a manner that is both fast and which integrates easily with point features. To achieve real-time operation, we present a fast straight-line detector that hypothesises and tests straight lines connecting detected seed points. We demonstrate that the resulting system provides good camera localisation and mapping in real-time on a standard workstation, using either line features alone, or lines and points combined.
Metal-inducible pathology in the liver, pancreas, and kidney of transgenic mice expressing SV40 early region genes. (1989)

Transgenic mice (SV-202) that carry the SV40 early region genes under the control of an inverted metallothionein promoter developed islet cell adenomas, hepatocellular carcinomas, and a generalized peripheral neuropathy. Both the islet cell adenomas and the hepatocellular carcinomas developed from the proliferation of T antigen-positive cells. However, T antigen expression was not seen in either the peripheral or central nervous systems. Stimulation of the metallothionein promoter with heavy metals altered the temporal onset of hepatic expression and broadened the distribution of oncogene expression to include exocrine pancreas and renal tubular epithelium. Although solid tumors were not seen in the exocrine pancreas or kidneys of SV-202 mice, all immunopositive tissues developed histologic changes. These results indicate that metallothionein-directed T antigen expression can induce abnormal cellular growth in a variety of tissues, and the distribution of these tissues can be manipulated with heavy metals.
Separate control of B lymphocyte early activation and proliferation in response to anti-IgM antibodies. (1985)

Resting B cells enter and progress through the G1 phase of the cell cycle in response to low concentrations (1 to 5 micrograms/ml) of anti-IgM antibodies. Commitment to enter S phase requires the presence of a fivefold to 50-fold higher concentration of anti-IgM. These and other results strongly suggest that two separately controlled events are involved in B cell activation. The current studies demonstrate that B cells incubated with high concentrations of anti-IgM from the initiation of culture become independent of additional anti-IgM approximately 10 hr before entry into S phase. We have designated this anti-IgM independent portion of the G1 phase of the cell cycle as G1 beta, whereas the earlier phase is referred to as G1 alpha. Furthermore, low concentrations of anti-IgM are sufficient for progress through early portions of G1 alpha, but high concentrations are required for the last 4 to 8 hr (G1 alpha') if the cells are to go through the rest of the cell cycle. Removal of anti-IgM at any time during G1 alpha causes prompt cessation of the size enlargement that accompanies progress through G1. Such cells retain their size and their relative place in G1 for periods of at least 17 hr and recommence movement through G1 alpha phase when anti-IgM is readded. Thus, B cells may exist in states of partial activation and must possess a mechanism to integrate the amount of stimulatory signal they have received; they enter a commitment period for S phase only when that signal passes some threshold value.
The renaturation of reduced chymotrypsinogen A in guanidine HCl. Refolding versus aggregation. (1978)

The refolding and reoxidation of fully reduced and denatured chymotrypsinogen A have been studied in the presence of low concentrations of guanidine HCl or urea. Renaturation yields of 60 to 70% were observed when the reoxidation was facilitated by mixtures of reduced and oxidized glutathione. Refolding occurred within a narrow range of denaturant concentration (1.0 to 1.3 M guanidine HCl and 2 M urea) in which the native protein was shown to be stable, and the reduced protein was shown to regain the correct disulfide pairing. Renatured chymotrypsinogen is indistinguishable from the native zymogen in chromatographic behavior, potential chymotryptic activity, sedimentation coefficient, and spectral properties. The kinetics of renaturation were determined. Some of the protein species obtained at various times of renaturation were characterized as incorrectly oxidized molecules which could be renatured by thiol-catalyzed interchange of disulfide bonds.
Acquired multicellular-mediated resistance to alkylating agents in cancer. (1993)

EMT-6 murine mammary tumor sublines highly resistant to cyclophosphamide, cis-diamminedichloro-platinum(II), or N,N',N"-triethylenethiophosphoramide were generated in vivo by sequential treatment of tumor-bearing mice with the respective drugs. Previous studies demonstrated the drug-resistant phenotypes of the sublines were not expressed in vitro when the cells were grown as monolayer cultures. We now show that expression of drug resistance--including patterns of cross-drug resistance observed in vivo--can be fully recapitulated in vitro when the cells are grown under in vivo-like, three-dimensional conditions--namely, as multicellular tumor spheroids. Moreover, the spheroids generated from all of the drug-resistant sublines manifested a much more compact structure. Immediate drug-sensitivity testing of single cells released by trypsin treatment from compact drug-resistant spheroids revealed that such cells lost much of their drug-resistant properties. The results suggest a possible mechanism of acquired drug resistance in tumors based on the response of a cell population (i.e., multicellular or tissue resistance) as opposed to classic (uni)cellular resistance mechanisms.
Ambient RF Energy-Harvesting Technologies for Self-Sustainable Standalone Wireless Sensor Platforms (2014)

In this paper, various ambient energy-harvesting technologies (solar, thermal, wireless, and piezoelectric) are reviewed in detail and their applicability in the development of self-sustaining wireless platforms is discussed. Specifically, far-field low-power-density energy-harvesting technology is thoroughly investigated and a benchmarking prototype of an embedded microcontroller-enabled sensor platform has been successfully powered by an ambient ultrahigh-frequency (UHF) digital TV signal (512-566 MHz) where a broadcasting antenna is 6.3 km away from the proposed wireless energy-harvesting device. A high-efficiency dual-band ambient energy harvester at 915 MHz and 2.45 GHz and an energy harvester for on-body application at 460 MHz are also presented to verify the capabilities of ambient UHF/RF energy harvesting as an enabling technology for Internet of Things and smart skins applications.
The Proteolytic Activation Systems of Complement (1981)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
5-Hydroxytryptamine and 5-Hydroxytryptamine-3 Receptor Antagonists (1991)

5-Hydroxytryptamine (5-HT) is present throughout the gastrointestinal tract, which acts as the major reservoir of this substance in the body. Its physiologic role has not been clearly established, although it seems likely that 5-HT is involved in the regulation of aspects of intestinal motility such as peristalsis and the migrating motor complex. In disease states the contribution of 5-HT is perhaps more clearly established, particularly its role in chemotherapy-induced emesis, in the carcinoid syndrome, and, possibly, in mediating the effect of some intestinal secretagogues, notably cholera toxin. Many of the functions of 5-HT in the gut have been elucidated as a result of the development of antagonists to 5-HT receptors. However, some of these compounds have 5-HT agonist activity as well as 5-HT receptor blocking activity, making interpretation of their effects in health and disease difficult. Nevertheless, 5-HT receptor antagonists are finding an important place in the management of the carcinoid syndrome and in chemotherapy-induced emesis and may well evolve as important agents for modulating gut motility and for inhibiting secretory states in the small and large intestine. The suggestion that 5-HT3 receptor antagonists might also modulate visceral sensation in the gut is of great interest because of their potential to relieve symptoms of functional bowel disorders such as pain, urgency, and bowel frequency.
Topology design and bandwidth allocation in ATM nets (1989)

The design of a P/S network embedded into a backbone facility network is discussed. The problem is formulated as a network optimization problem where a congestion measure based on the average packet delay is minimized, subject to capacity constraints posed by the underlying facility trunks. The variables in this problem are the routing on the express pipes (i.e. the channels that interconnect the P/S modes) and the allocation of bandwidth to such pipes. An efficient algorithm is presented for the solution of the above problem and it is applied to some representative examples. It is shown that for some test cases the congestion measure is substantially reduced with respect to the values obtained when the embedded topology is kept identical to the backbone topology. Dynamic reconfiguration schemes where the embedded topology is periodically adjusted to track the fluctuations in traffic requirements are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A BAW antenna duplexer for the 1900 MHz PCS band (2003)

Miniature antenna duplexers are used in modem cellular telephone handsets. We present a new solution utilizing micro-machined, thin Film Bulk Acoustic Resonators (FBAR). A 1900 MHz duplexer with dimensions 5/spl times/8/spl times/2 mm is achieved. The duplexer, a three port device, achieves low loss from transmitter (Tx) to antenna (Ant) ports with a filter at 1880 MHz and from Ant to receiver (Rx) ports with a filter at 1960 MHz. High isolation from Tx to Rx port is achieved by coupling the two filters with a quarter wavelength line. Filter bandwidth is /spl sim/3%. The filters are realized as 21/2 or 31/2 section ladder connected FBARs with the shunt FBARs tuned 3% lower in frequency than the series FBARs to provide a flat pass band. Individual FEAR resonators are free membranes formed of Aluminum Nitride piezoelectric and metal electrode films micro-machined on a silicon substrate. Resonators with electro-acoustic coupling k/sub t//sup 2//spl sim/3 to 5% and Q/spl sim/300 are achieved. Filters fabricated in this manner achieve minimum insertion loss /spl sim/1 dB, out-of-band rejection of /spl sim/20 dB, 60 MHz bandwidth, and an input power capability of 2 Watts. Duplexers are assembled from one each Tx and Rx filter, and a lumped /spl lambda//4 line of two inductors and a capacitor. These duplexers have insertion loss <2 dB in the pass bands, and Tx to Rx isolation >45 dB.
Safety and Pharmacokinetics of Hyperimmune Anti-Human Immunodeficiency Virus (HIV) Immunoglobulin Administered to HIV-Infected Pregnant Women and Their Newborns (1997)

The pharmacokinetics and safety of hyperimmune anti-human immunodeficiency virus (HIV) intravenous immunoglobulin (HIVIG) were evaluated in the first 28 maternal-infant pairs enrolled in a randomized, intravenous immunoglobulin (IVIG)-controlled trial of HIVIG maternal-infant HIV transmission prophylaxis. Using 200 mg/kg, mean half-life and volume of distribution (Vd) in women were 15 days and 72 mL/kg, respectively, after one and 32 days and 154 mL/kg after three monthly infusions, with stable 4 mL/kg/day clearance. Transplacental passage occurred. Newborn single-dose half-life, Vd, and clearance were 30 days, 143 mL/kg, and 4 mL/kg/day, respectively. HIVIG rapidly cleared maternal serum immune complex-dissociated p24 antigen, and plasma HIV-1 RNA levels were stable. Mild to moderate adverse clinical effects occurred in 2 of 103 maternal and 2 of 25 infant infusions. No adverse hematologic, blood chemistry, or immunologic effects were seen. HIVIG is well-tolerated in HIV-infected pregnant women and their newborns, clears antigenemia, crosses the placenta, and exhibits pharmacokinetics similar to those of other immunoglobulin preparations.
Design of a multi-mode pipelined multiplier for floating-point applications (2002)

The design of a VLSI multimode pipelined multiplier is presented. The multiplier is designed to accept three types of operands: IEEE FP (floating point) double- and single-format and 32-b two's complement integers. The multiplier complies fully with the IEEE standard for floating point arithmetic, with the exception of denormalized numbers. All rounding modes and exception handling are supported. The high performance of the multiplier is made possible by the use of octal Booth encoding of the multiplier to reduce the number of partial products. In addition, a novel fast carry adder, the optimized-carry-multiplexed adder, is used to accelerate the critical carry propagation paths. The multiplier is designed to operate at 40 MHz and is pipelined to allow a new operation to begin every clock cycle. Thus, 40-MFLOPS operation is possible in double-precision and integer modes. In single-precision mode, two operations are performed in parallel, yielding 80-MFLOPS performance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Activation of Cardiac Progenitor Cells Reverses the Failing Heart Senescent Phenotype and Prolongs Lifespan (2008)

Heart failure is the leading cause of death in the elderly, but whether this is the result of a primary aging myopathy dictated by depletion of the cardiac progenitor cell (CPC) pool is unknown. Similarly, whether current lifespan reflects the ineluctable genetic clock or heart failure interferes with the genetically determined fate of the organ and organism is an important question. We have identified that chronological age leads to telomeric shortening in CPCs, which by necessity generate a differentiated progeny that rapidly acquires the senescent phenotype conditioning organ aging. CPC aging is mediated by attenuation of the insulin-like growth factor-1/insulin-like growth factor-1 receptor and hepatocyte growth factor/c-Met systems, which do not counteract any longer the CPC renin-angiotensin system, resulting in cellular senescence, growth arrest, and apoptosis. However, pulse-chase 5-bromodeoxyuridine-labeling assay revealed that the senescent heart contains functionally competent CPCs that have the properties of stem cells. This subset of telomerase-competent CPCs have long telomeres and, following activation, migrate to the regions of damage, where they generate a population of young cardiomyocytes, reversing partly the aging myopathy. The senescent heart phenotype and heart failure are corrected to some extent, leading to prolongation of maximum lifespan.
Standard Operating Procedures for Female Orgasmic Disorder: Consensus of the International Society for Sexual Medicine (2012)

Evidence-based, practice recommendations for the treatment of FOD are provided that will hopefully inform clinical decision making for those treating this common condition.
Hypersensitivity reactions from taxol. (1990)

Taxol is an antitumor agent in clinical trial that has been shown to have activity against advanced ovarian carcinoma and melanoma. Hypersensitivity reactions (HSRs) have been one of the toxicities observed with administration of this drug. Of 301 patients treated, 32 patients have had definite (27 patients) or possible (five patients) hypersensitivity reactions to taxol. All but one patient had the reaction from the first or second exposure to this agent. Reactions occurred at a variety of doses and were characterized most frequently by dyspnea, hypotension, bronchospasm, urticaria, and erythematous rashes. Thirteen (41%) patients had received premedication designed to prevent such toxicity; nevertheless, they sustained HSRs. Prolonging the drug infusion appears to have somewhat reduced, but not obviated, the risk of HSRs. The cause (taxol itself or its excipient Cremophor EL; Badische Anilin und Soda-Fabrik AG [BASF], Ludwigshafen, Federal Republic of Germany) and the mechanism of these reactions to taxol are unknown. We provide guidelines to prevent or minimize such toxicity and treat reactions if they still occur.
Sodium channels and pain (1999)

Although it is well established that hyperexcitability and/or increased baseline sensitivity of primary sensory neurons can lead to abnormal burst activity associated with pain, the underlying molecular mechanisms are not fully understood. Early studies demonstrated that, after injury to their axons, neurons can display changes in excitability, suggesting increased sodium channel expression, and, in fact, abnormal sodium channel accumulation has been observed at the tips of injured axons. We have used an ensemble of molecular, electrophysiological, and pharmacological techniques to ask: what types of sodium channels underlie hyperexcitability of primary sensory neurons after injury? Our studies demonstrate that multiple sodium channels, with distinct electrophysiological properties, are encoded by distinct mRNAs within small dorsal root ganglion (DRG) neurons, which include nociceptive cells. Moreover, several DRG neuron-specific sodium channels now have been cloned and sequenced. After injury to the axons of DRG neurons, there is a dramatic change in sodium channel expression in these cells, with down-regulation of some sodium channel genes and up-regulation of another, previously silent sodium channel gene. This plasticity in sodium channel gene expression is accompanied by electrophysiological changes that poise these cells to fire spontaneously or at inappropriate high frequencies. Changes in sodium channel gene expression also are observed in experimental models of inflammatory pain. Thus, sodium channel expression in DRG neurons is dynamic, changing significantly after injury. Sodium channels within primary sensory neurons may play an important role in the pathophysiology of pain.