gebhart/patrepeval huggingface dataset:
Inhibition of .alpha.-chymotrypsin by D-tryptophan amide covalently bound to macromolecular carriers: specific, steric, and electrostatic effects (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInhibition of .alpha.-chymotrypsin by D-tryptophan amide covalently bound to macromolecular carriers: specific, steric, and electrostatic effectsShmaryahu Blumberg and Ephraim Katchalski-KatzirCite this: Biochemistry 1979, 18, 10, 2126–2133Publication Date (Print):May 1, 1979Publication History Published online1 May 2002Published inissue 1 May 1979https://pubs.acs.org/doi/10.1021/bi00577a044https://doi.org/10.1021/bi00577a044research-articleACS PublicationsRequest reuse permissionsArticle Views50Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
New Biodegradable Thermogelling Copolymers Having Very Low Gelation Concentrations (2006)

New biodegradable multiblock amphiphilic and thermosensitive poly(ether ester urethane)s consisting of poly[(R)-3-hydroxybutyrate] (PHB), poly(ethylene glycol) (PEG), and poly(propylene glycol) (PPG) blocks were synthesized, and their aqueous solutions were found to undergo a reversible sol-gel transition upon temperature change at very low copolymer concentrations. The multiblock poly(ether ester urethane)s were synthesized from diols of PHB, PEG, and PPG using 1,6-hexamethylene diisocyanate as a coupling reagent. The chemical structures and molecular characteristics of the copolymers were studied by GPC, 1H NMR, 13C NMR, and FTIR. The thermal stability of the poly(PEG/PPG/PHB urethane)s was studied by thermogravimetry analysis (TGA), and the PHB contents were calculated based on the thermal degradation profile. The results were in good agreement with those obtained from the 1H NMR measurements. The poly(PEG/PPG/ PHB urethane)s presented better thermal stability than the PHB precursors. The water soluble poly(ether ester urethane)s had very low critical micellization concentration (CMC). Aqueous solutions of the new poly(ether ester urethane)s underwent a sol-gel-sol transition as the temperature increased from 4 to 80 degrees C, and showed a very low critical gelation concentration (CGC) ranging from 2 to 5 wt %. As a result of its multiblock architecture, a novel associated micelle packing model can be proposed for the sol-gel transition for the copolymer gels of this system. The new material is thought to be a promising candidate for injectable drug systems that can be formulated at low temperatures and forms a gel depot in situ upon subcutaneous injection.
Abnormal patterns of DNA methylation in human neoplasia: potential consequences for tumor progression. (1991)

An imbalance of DNA methylation, involving widespread hypomethylation, regional hypermethylation and increased cellular capacity for methylation, is characteristic of human neoplasia. This imbalance begins in preneoplastic cells and becomes more extensive throughout subsequent stages of tumor progression. In normal cells, a primary function of DNA methylation may be to modulate compartmentalization of DNA to ensure that regional areas of transcriptionally active chromatin replicate earlier than the bulk transcriptionally inactive chromatin. We argue here that the altered methylation patterns observed during tumor progression, especially regional hypermethylation, may mark--or even help to establish--abnormalities of chromatin organization. In turn, these changes in chromatin structure may, through direct transcriptional inactivation of genes, predisposition to mutations, and allelic deletions, mediate the progressive losses of gene expression associated with tumor development.
Retrieving collocations from text: Xtract (1993)

Natural languages are full of collocations, recurrent combinations of words that co-occur more often than expected by chance and that correspond to arbitrary word usages. Recent work in lexicography indicates that collocations are pervasive in English; apparently, they are common in all types of writing, including both technical and nontechnical genres. Several approaches have been proposed to retrieve various types of collocations from the analysis of large samples of textual data. These techniques automatically produce large numbers of collocations along with statistical figures intended to reflect the relevance of the associations. However, noue of these techniques provides functional information along with the collocation. Also, the results produced often contained improper word associations reflecting some spurious aspect of the training corpus that did not stand for true collocations. In this paper, we describe a set of techniques based on statistical methods for retrieving and identifying collocations from large textual corpora. These techniques produce a wide range of collocations and are based on some original filtering methods that allow the production of richer and higher-precision output. These techniques have been implemented and resulted in a lexicographic tool, Xtract. The techniques are described and some results are presented on a 10 million-word corpus of stock market news reports. A lexicographic evaluation of Xtract as a collocation retrieval tool has been made, and the estimated precision of Xtract is 80%.
The Preparation of Boron Hydrides by the Reduction of Boron Halides (1949)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation of Boron Hydrides by the Reduction of Boron HalidesDallas T. HurdCite this: J. Am. Chem. Soc. 1949, 71, 1, 20–22Publication Date (Print):January 1, 1949Publication History Published online1 May 2002Published inissue 1 January 1949https://pubs.acs.org/doi/10.1021/ja01169a007https://doi.org/10.1021/ja01169a007research-articleACS PublicationsRequest reuse permissionsArticle Views235Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Comparative Assessment of Genotyping Methods for Epidemiologic Study of Burkholderia cepacia Genomovar III (2002)

ABSTRACT We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory power of these techniques were evaluated, and the results were compared to each other and to data obtained in previous studies by using multilocus restriction typing (MLRT). All methods showed excellent typeability. PFGE with Spe I was more reproducible than RAPD and BOX-PCR fingerprinting. The discriminatory power of the methods was variable, with PFGE and RAPD typing having a higher index of discrimination than BOX-PCR fingerprinting. In general, the results obtained by PFGE, BOX-PCR fingerprinting, and MLRT were in good agreement. Our data indicate that different genomic-based methods can be used to type B. cepacia genomovar III isolates depending on the situation and the epidemiologic question being addressed. PFGE and RAPD fingerprinting are best suited to addressing small-scale studies (i.e., local epidemiology), whereas BOX-PCR fingerprinting is more appropriate for large-scale studies (i.e., global epidemiology). In this regard, BOX-PCR fingerprinting can be considered a rapid and easy alternative to MLRT.
GENETIC ENGINEERING OF PLANT LIPIDS (1999)

Vegetable oils are a major component of human diets, comprising as much as 25% of average caloric intake. Until recently, it was not possible to exert significant control over the chemical composition of vegetable oils derived from different plants. However, the advent of genetic engineering has provided novel opportunities to tailor the composition of plant-derived lipids so that they are optimized with respect to food functionality and human dietary needs. In order to exploit this new capability, it is essential for food scientists and nutritionists to define the lipid compositions that would be most desirable for various purposes.
Differential proteomic serum pattern of low molecular weight proteins expressed by adenocarcinoma lung cancer patients. (2005)

Based on the assumption that proteins can emanate from tumour to serum, we investigated whether serum low molecular weight proteins (LMW) can discriminate lung cancer patients from healthy donors. Pooled sera from 20 lung cancer patients matched in sex (men), histological type (adenocarcinoma) and stage (IIIB and IV) and from 20 healthy donors (men) were submitted to 2-DE coupled to MALDI-TOF peptide mass fingerprinting. Results of 2D-E/ MALDI-TOF showed five up regulated proteins (immunoglobulin lambda chain, transthyretin monomer, haptoglobin-alfa 2 and two isoforms of serum amyloid protein) and one down regulated (fragment of apolipoprotein A-I) in patients. All differentially expressed proteins, except immunoglobulin, may be acting as a non-specific sign of inflammation in cancer and transthyretin monomer may act as a possible blood marker to CSF barrier disruption that occurs, e.g., in cerebral metastasis. In conclusion, this work shows the usefulness of 2D-gel electrophoresis and mass spectrometry proteomic techniques for the identification of up- and down-regulated proteins in serum from adenocarcinoma lung cancer patients.
aroA -Deficient Salmonella enterica Serovar Typhimurium Is More Than a Metabolically Attenuated Mutant (2016)

Recombinant attenuated bacterial vector systems based on genetically engineered Salmonella have been developed as highly potent vaccines. Due to the pathogenic properties of Salmonella, efficient attenuation is required for clinical applications. Since the hallmark study by Hoiseth and Stocker in 1981 (S. K. Hoiseth and B. A. D. Stocker, Nature 291:238-239, 1981, http://dx.doi.org/10.1038/291238a0), the auxotrophic ΔaroA mutation has been generally considered safe and universally used to attenuate bacterial strains. Here, we are presenting the remarkable finding that a deletion of aroA leads to pronounced alterations of gene expression, metabolism, and cellular physiology, which resulted in increased immunogenicity, virulence, and adjuvant potential of Salmonella. These results suggest that the enhanced immunogenicity of aroA-deficient Salmonella strains might be advantageous for optimizing bacterial vaccine carriers and immunotherapy. Accordingly, we demonstrate a superior performance of ΔaroA Salmonella in bacterium-mediated tumor therapy. In addition, the present study highlights the importance of a functional shikimate pathway to sustain bacterial physiology and metabolism.
Electrochemical Capacitance of a Nanoporous Composite of Carbon Nanotubes and Polypyrrole (2002)

This work reports the supercapacitive properties of electrochemically grown composite films of multiwalled carbon nanotubes (MWNT) and polypyrrole (PPy), a conducting polymer. Scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy revealed that the nanoporous three-dimensional arrangement of PPy-coated MWNTs in these films facilitated improved electron and ion transfer relative to pure PPy films. The low-frequency capacitance was measured for films of varying thickness, revealing specific capacitances per mass (Cmass) and geometric area (Carea) as high as 192 F g-1 and 1.0 F cm-2, respectively. Rates of charge and discharge about an order of magnitude faster than similarly prepared pure PPy films were also observed.
On-Chip Digital Inductor Current Sensor for Monolithic Digitally Controlled DC-DC Converter (2013)

Conventionally, inductor current is sensed and quantized before it can be used by digitally controlled DC-DC converters. These are intuitively done by using two separated functional blocks-an analog current sensor and an ADC. A few research works have investigated other ways of obtaining the inductor current information in the digital domain. This paper proposes an on-chip digital inductor current sensor that combines both the inductor current-sensing and quantization into a single functional block. In this way, the redundancies found in the conventional approach can be reduced and optimizations can be made to save chip area and power consumption. An 8-bit digital inductor current sensor has been designed and fabricated with a UMC 0.13 μm CMOS process. The measurement results show that the digital sensor can provide digital inductor current information with a conversion time of 225 ns. This can be used by a buck converter with a switching frequency up to 4 MHz. The digital sensor has linear and monotonic input-output transfer curve properties with an LSB of 6.79 mA. It consumes current of 700 μA at 1.2 V supply voltage.
AMERSHAM PHARMACIA BIOTECH &AMP; SCIENCE PRIZE:The Rotary Enzyme of the Cell: The Rotation of F1-ATPase (1998)

The winner of this year's Amersham Pharmacia Biotech & Science Prize is Hiroyuki Noji, from Japan. In his winning essay , Noji describes his thesis experiments in which he visualized the rotation of the gamma subunit of F1-ATPase by attaching long actin molecules. This result proved that the gamma subunit does indeed rotate within the bearing-like structure created by the alpha and beta subunits. F1-ATPase is a true molecular motor, converting chemical to mechanical energy on a very small scale.
Interleukin-7 Is a Survival Factor for CD4+ CD25+ T-Cells and Is Expressed by Diabetes-Suppressive Dendritic Cells (2006)

Dendritic cells can facilitate allograft survival and prevent autoimmunity via direct and indirect cell-mediated mechanisms. Recent studies demonstrate that immunoregulatory dendritic cells (iDCs) confer immune hyporesponsiveness in part through CD4(+) CD25(+) T regulatory cells (Tregs). Herein, we provide evidence to support the hypothesis that dendritic cells derived from NOD mice and engineered ex vivo to exhibit suppressed expression of the CD40, CD80, and CD86 costimulatory molecules motivate an increase in the prevalence of regulatory CD4(+) CD25(+) T-cells via interleukin (IL)-7. Unlike control dendritic cells, these dendritic cells expressed significant levels of IL-7. Exogenous addition of IL-7 to NOD T-cells did not promote expansion or proliferation, but instead selectively maintained the number of CD4(+) CD25(+) T-cells by inhibiting activation of apoptosis in these cells. In vitro, IL-7 receptor alpha-chain (IL-7Ralpha) was expressed at significantly higher levels on CD4(+) CD25(+) T-cells compared with CD4(+) CD25(-) T-cells irrespective of resting or stimulated state. In vivo, CD4(+) CD25(+) T-cells obtained from NOD-scid mice reconstituted with ex vivo engineered iDCs and NOD splenocytes expressed significantly higher levels of IL-7Ralpha compared with levels in the CD4(+) CD25(-) subset, especially in diabetes-suppressive dendritic cell-administered NOD-scid recipients. Taken together, our data suggest a novel mechanism by which iDCs delay autoimmunity through the CD4(+) CD25(+) Treg pathway and suggest IL-7 as a survival factor for these putative Tregs, which express the alpha-chain of its receptor at considerably higher levels than CD4(+) CD25(-) T-cells.
Regulation of estrogen receptor gene mediated by promoter B responsible for its enhanced expression in human breast cancer (1999)

We have previously reported that transcription from a distal promoter (promoter B) of the estrogen receptor alpha (ERalpha) gene is responsible for the increased expression of ERalpha in human breast carcinomas. This paper first characterized the promoter B region in terms of transient transfection experiments with luciferase using MCF-7 cells. Gradual deletions from the 5'-end of promoter B resulted in a decrease in promoter activity corresponding to the deleted lengths; a deletion of 39 bp in a non-coding exon 1a, drastically diminished the activity, indicating existence of an important cis -element. Furthermore, electrophoretic mobility shift assay and subsequent mutational analysis indicated that this element containing nucleotide sequence CTGGAAAG forms a specific DNA-protein complex. This element was capable of transactivating a heterogeneous SV40 promoter in MCF-7 cells, confirming that the element is a transcriptional enhancer; the trans -acting factor binding to the element was named ERBF-1 (estrogen receptor promoter B associated factor-1). The ERBF-1 was exclusively expressed in those cells expressing ERalpha mRNA transcribed from promoter B. Our findings indicate that ERBF-1 plays an important role in the expression of the ERalpha gene transcribed from promoter B, which is selectively utilized in breast cancer.
[Randomized phase II trial on escalated doses of Rh-endostatin (YH-16) for advanced non-small cell lung cancer]. (2006)

Rh-endostatin may have anti-tumor activity with high clinical benefit rate and is well tolerated in pretreated advanced NSCLC patients. The dose of 7.5 mg x (m(2))(-1) x d(-1) is clinically recommended.
Mutation rate varies among alleles at a microsatellite locus: Phylogenetic evidence (1996)

The understanding of the mutational mechanism that generates high levels of variation at microsatellite loci lags far behind the application of these genetic markers. A phylogenetic approach was developed to study the pattern and rate of mutations at a dinucleotide microsatellite locus tightly linked to HLA-DQB1 (DQCAR). A random Japanese population (n = 129) and a collection of multiethnic samples (n = 941) were typed at the DQB1 and DQCAR loci. The phylogeny of DQB1 alleles was then reconstructed and DQCAR alleles were superimposed onto the phylogeny. This approach allowed us to group DQCAR alleles that share a common ancestor. The results indicated that the DQCAR mutation rate varies drastically among alleles within this single microsatellite locus. Some DQCAR alleles never mutated during a long period of evolutionary time. Sequencing of representative DQCAR alleles showed that these alleles lost their ability to mutate because of nucleotide substitutions that shorten the length of uninterrupted CA repeat arrays; in contrast, all mutating alleles had relatively longer perfect CA repeat sequences.
Autologous mesenchymal stem cells for the treatment of secondary progressive multiple sclerosis: an open-label phase 2a proof-of-concept study (2012)

Medical Research Council, Multiple Sclerosis Society of Great Britain and Northern Ireland, Evelyn Trust, NHS National Institute for Health Research, Cambridge and UCLH Biomedical Research Centres, Wellcome Trust, Raymond and Beverly Sackler Foundation, and Sir David and Isobel Walker Trust.
Most germ-line mutations in the nevoid basal cell carcinoma syndrome lead to a premature termination of the PATCHED protein, and no genotype-phenotype correlations are evident. (1997)

The human homologue of the Drosophila segment polarity gene patched is implicated in the development of nevoid basal cell carcinoma syndrome (NBCCS) and in the genesis of sporadic basal cell carcinomas. In order to examine the phenotypic variability in NBCCS and to highlight functionally important domains of the PTCH protein, we have now screened 71 unrelated NBCCS individuals for mutations in the PTCH exons. We identified 28 mutations that are distributed throughout the entire gene, and most (86%) cause protein truncation. As part of this analysis, we demonstrate that failure of one NBCCS family to show clear linkage to chromosome 9q22.3-31 is most likely due to germinal mosaicism. We have identified three families bearing identical mutations with variable phenotypes, suggesting phenotypic variability in NBCCS is a complex genetic event. No phenotype genotype correlation between the position of truncation mutations and major clinical features was evident. Two missense mutations have been identified, and their location within transmembrane domains supports the notion that PTCH may have a transport function. The preponderance of truncation mutants in the germ line of NBCCS patients suggests that the developmental defects associated with the disorder are most likely due to haploinsufficiency.
Latency, histology, and antigenicity of tumors induced by ultraviolet light in three inbred mouse strains. (1977)

These data are collected from studies looking at Chinese training courses for African officials, Chinese trade in agricultural commodities with Africa, and registered Chinese investments in Africa. These formed an important contextualisation of our research in the four case study countries which we then built upon in our fieldwork. Those final analyses and interpretations of the data are also included as data here in the form of working papers. Based on qualitative research methods, they present the reality on the ground which can often be quite different to the image presented by the raw figures, often published by government departments. The “China and Brazil in African Agriculture” (CBAA) project will explore the new development cooperation engagements in agriculture across four African countries. The project will examine the politics of aid and investment policy in China and Brazil, exploring how understandings of agricultural development are translated in aid and investment projects. The project will be carried out through an innovative international partnership connecting researchers from institutions in the UK and Africa, already linked through the IDS-led Future Agricultures Consortium, with colleagues from China and Brazil. The research will begin with a mapping phase that will generate a geo-referenced database of Chinese and Brazilian agricultural development cooperation projects in Ethiopia, Ghana, Mozambique and Zimbabwe. This will be followed by in-depth case studies of a sample of these projects, examining the ways in which experience and expertise from China and Brazil engage with the realities of African agriculture and the perspectives of African scientists and farmers. Comparative analysis across projects, countries and types of intervention will address the question of whether a new paradigm of development cooperation is emerging, and assess the implications for the future of aid and investment policy.