gebhart/patrepeval huggingface dataset:
Effects of particulate beta-1,3 glucan on human, rat, and guinea pig complement activity. (1983)

Particulate glucan, a beta-1,3-linked polyglucose derived from Saccharomyces cerevisiae, has been demonstrated to have a wide range of immunopotentiating effects. Glucan administration is associated with the modification of a variety of experimentally induced infectious disease states as well as the inhibition of growth of implantable and spontaneous tumors. The present study was designed to evaluate the effect of glucan upon activation of the complement system in rats and guinea pigs. Additional studies were performed to determine the in vitro activating effect of glucan and zymosan on complement activity of human serum. Glucan activated both the classical pathway of normal human sera and the alternate pathways in C2hu-deficient sera in vitro releasing anaphylatoxins such as C3a. The intravenous injection of glucan activated the alternate pathway of guinea pig plasma. The influence of glucan on complement depletion induced by cobra venom factor (CVF) was also ascertained. Complement activation by glucan may contribute, in part, to the enhanced resistance of the host against tumor growth as well as infectious episodes.
Further Characterization of Proteins Associated with Elastic Fiber Microfibrils Including the Molecular Cloning of MAGP-2 (MP25) (1996)

Together with the 31-kDa microfibril-associated glycoprotein (MAGP), four polypeptides designated MP340 (340 kDa), MP78 (78 kDa), MP70 (70 kDa), and MP25 (25 kDa) have previously been identified in tissue extracts designed specifically to solubilize the microfibrillar component of elastic fibers. In the present study, both MP78 and MP70 were shown to be forms of a protein which is closely related to the human protein beta ig-h3, and MP340 was confirmed to be the bovine form of fibrillin-1. Peptide sequences from MP25 proved to be unique, and affinity-purified anti-MP25 antibodies were shown, by immunofluorescence and immunoelectron microscopy, to localize specifically to the elastin-associated microfibrils. This confirmed that MP25 was a distinct component of these structures. Expression screening of nuchal ligament cDNA libraries yielded a cDNA, cM10A (770 base pairs) which encodes amino acid sequences matching those of the MP25 peptides. Further library screening with cM10A identified cDNAs which encode the complete primary structures of bovine and human MP25. Bovine and human MP25 were found to be around 80% homologous and contain 170 and 173 amino acids, respectively. Data base searches revealed that MP25 had significant similarity of structure only with MAGP, indicating that the two proteins form a new family of microfibrillar proteins. In acknowledgment, MP25 has been formally renamed MAGP-2, and MAGP is referred to as MAGP-1. The close similarity between the two proteins (57%) is confined to a central region of 60 amino acids where there is precise alignment of 7 cysteine residues. Elsewhere the MAGP-2 molecule is rich in serine and threonine residues and contains an RGD motif. MAGP-2 lacks the proline-, glutamine-, and tyrosine-rich sequences and a hydrophobic carboxyl terminus, characteristic of MAGP-1. These structural differences suggest that MAGP-2 has some functions which are distinct from those of MAGP-1. The locus of the human MAGP-2 gene was identified on chromosome 12 in the region of 12p12.3-12p13.1.
Plasma concentrations of disodium cromoglycate after various inhalation methods in healthy subjects (1999)

These findings may have important implications for the evaluation of inhalation treatment with DSCG for bronchial asthma.
Residential energy monitoring and computerized surveillance via utility power flows (1989)

The author notes that all novel technologies have the potential to affect society in a complex manner, with both beneficial and detrimental consequences. He considers an illustrative case study: a nonintrusive appliance load monitoring technique that can provide vital information to help avoid future energy crises, but can also be used for surveillance purposes. He notes that there appears to be a significant potential for the technology to be abused. The danger that the technology might eventually lead to an erosion of civil liberties and privacy rights leaves its developers in an ethical quandary. The author examines how this technology should be controlled.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Advanced Biotelemetry Systems for Space Life Sciences (1994)

The Sensors 2000! Program at NASA-Ames Research Center is developing an Advanced Biotelemetry System (ABTS) for Space Life Sciences applications. This modular suite of instrumentation is planned to be used in operational spaceflight missions, ground-based research and development experiments, and collaborative, technology transfer and commercialization activities. The measured signals will be transmitted via radio-frequency (RF), electromagnetic or optical carriers and direct-connected leads to a remote ABTS receiver and data acquisition system for data display, storage, and transmission to Earth. Intermediate monitoring and display systems may be hand held or portable, and will allow for personalized acquisition and control of medical and physiological data.
A Human FcγRI/CD64 Transgenic Model for In Vivo Analysis of (Bispecific) Antibody Therapeutics (1995)

The human high-affinity IgG receptor, hFcγRI (CD64), is exclusively expressed on myeloid cells, where it serves an important role as a (cytotoxic) trigger molecule. To establish an in vivo model for analysis of the role of hFcγRI in immunity, we developed a novel transgenic mouse model. The human FcγRIA gene, with endogenous regulatory sequences, was used to generate two lines of transgenic FVB/N mice. Immunohistochemical and flow cytometric studies showed that hFcγRI expression was restricted to myeloid cells. Monocytes, macrophages, and polymorphonuclear neutrophils (PMN) expressed physiologic hFcγRI levels, whereas lymphocytes and mast cells lacked expression. Human FcγRI expression was regulated in vivo by the cytokines IFN-γ (exactly as in humans) and IL-10. The transgenic receptor proved functional and bound human tumor cells via anti-hFcγRI-based bispecific antibodies. hFcγRI could, furthermore, be efficiently targeted in vivo by CD64 antibodies. These data demonstrate that the hFcγRI transgenic mouse model closely parallels the situation in humans. This mouse model seems useful for in vivo evaluation of the therapeutic potential of novel bispecific reagents in tumor and infection models.
Purification and Properties of 7β-(4-Carboxybutanamido)-cephalosporanic Acid Acylase Produced by Mutants Derived fromPseudomonas (1981)

7β-(4-Carboxybutanamido)cephalosporanic acid acylase (penicillin amidohydrolase, EC 3.5.1.11) was crystallized from cell-free extracts of a mutant derived from Pseudomonas SY-77-1. Purification of the enzyme was performed by a procedure involving ammonium sulfate fractionation and column chromatographies on DEAE-Sephadex, TEAE-cellulose and Sephadex G-200. The crystalline enzyme was homogeneous on polyacrylamide gel disc electrophoresis. The molecular weight of the acylase was estimated to be 1.3 × 105 by gel filtration. The enzyme was fully active at pH above 6.5 and was highly stable at a pH range of 6.0 to 8.0 and below 38°C. The Michaelis-Menten constant (Km) and Vmax for 7β-(4-carboxybutanamido)cephalosporanic acid were 0.16mM and 4.91 μmol/min/mg-protein, respectively. It was also indicated that this enzyme-protein occupied 2.3% of the dry-cell weight.
The M2 Ectodomain Is Important for Its Incorporation into Influenza A Virions (1998)

M2 is an integral protein of influenza A virus that functions as an ion channel. The ratio of M2 to HA in influenza A virions differs from that found on the cell surface, suggesting selective incorporation of M2 and HA into influenza virions. To examine the sequences that are important for M2 incorporation into virions, we used an incorporation assay that involves expressing M2 from a plasmid, transfecting the plasmid into recipient cells, and then infecting those cells with influenza virus. To test the importance of the different regions of the protein (extracellular, transmembrane, and cytoplasmic) in determining M2 incorporation, we created chimeric mutants of M2 and Sendai virus F proteins, exchanging corresponding extracellular, transmembrane, and cytoplasmic domains. Of the six possible chimeric mutants, only three were expressed on the cell surface. Of these three chimeric proteins, only one mutant (with the extracellular domain from M2 and the rest from F) was incorporated into influenza virions. These results suggest that the extracellular domain of M2 is important for its incorporation into virions.
Quantitative assessment of liver fat with magnetic resonance imaging and spectroscopy (2011)

Abstract Hepatic steatosis is characterized by abnormal and excessive accumulation of lipids within hepatocytes. It is an important feature of diffuse liver disease, and the histological hallmark of nonalcoholic fatty liver disease (NAFLD). Other conditions associated with steatosis include alcoholic liver disease, viral hepatitis, human immunodeficiency virus (HIV) and genetic lipodystrophies, cystic fibrosis liver disease, and hepatotoxicity from various therapeutic agents. Liver biopsy, the current clinical gold standard for assessment of liver fat, is invasive and has sampling errors, and is not optimal for screening, monitoring, clinical decision‐making, or well suited for many types of research studies. Noninvasive methods that accurately and objectively quantify liver fat are needed. Ultrasound (US) and computed tomography (CT) can be used to assess liver fat but have limited accuracy as well as other limitations. Magnetic resonance (MR) techniques can decompose the liver signal into its fat and water signal components and therefore assess liver fat more directly than CT or US. Most MR techniques measure the signal fat‐fraction (the fraction of the liver MR signal attributable to liver fat), which may be confounded by numerous technical and biological factors and may not reliably reflect fat content. By addressing the factors that confound the signal fat‐fraction, advanced MR techniques measure the proton density fat‐fraction (the fraction of the liver proton density attributable to liver fat), which is a fundamental tissue property and a direct measure of liver fat content. These advanced techniques show promise for accurate fat quantification and are likely to be commercially available soon. J. Magn. Reson. Imaging 2011;. © 2011 Wiley‐Liss, Inc.
Mechanisms of Recombination: Lessons fromE. coli (2008)

The genetics and biochemistry of genetic recombination in E. coli has been studied for over four decades and provides a useful model system to understand recombination in other organisms. Here we provide an overview of the mechanisms of recombination and how such processes contribute to DNA repair. We describe the E. coli functions that are known to contribute to these mechanisms, step by step, and summarize their biochemical properties in relation to the role these proteins play in vivo. We feature areas of investigation that are newly emerging, as well as work that provides a historical perspective to the field. Finally, we highlight some of the questions that remain unanswered.
Cycloadditions dipolaires‐1,3 II. Addition des diarylnitrilimines au N‐methyl‐indole. Etude expérimentale et essai d'interprétation (1977)

Abstract On étudie la réactivite du N ‐méthylindole vis‐à‐vis de plusieurs diarylnitrilimines. Les cycloadduits obtenus sont des dérivés du méthyl‐8 dihydro‐3a,8a pyrazolo [3,4‐ b ] indole. La structure proposée est en accord avec les données analytiques et spectrales. Elle est confirmée par voie chimique. Une interprétation fondee sur la méthode de perturbation au second ordre des propriétés des orbitales frontières rend compte de la régiospécificité observée.
Early and late events in Fc epsilon RI signal transduction in human cultured mast cells (1997)

Protein tyrosine phosphorylation and other biochemical events have been shown to occur after cross-linking of Fc epsilonRI in rodent mast cells. To investigate the mechanism of Fc epsilonRI signal transduction in human mast cells, we used human cultured mast cells (HCMC) generated from cord blood cells in the presence of recombinant human stem cell factor and IL-6. We found that on cross-linking of Fc epsilonRI: 1) HCMC released histamine; 2) rapid tyrosine phosphorylation of multiple cellular substrates, including Syk, HS1, c-Cbl, ERK-1, and ERK-2, was observed; 3) intracellular Ca2+ and inositol phosphate production were increased within the first minute after Fc epsilonRI cross-linking; and 4) genistein, a tyrosine kinase inhibitor, inhibited both protein tyrosine phosphorylation and histamine release in a dose-dependent manner. These results were consistent with previous studies in rodent mast cells. In contrast, no tyrosine phosphorylation of phospholipase C gamma1 and Btk (Bruton's tyrosine kinase) were observed in our experimental conditions. These results suggest that the greater part of the early and late signaling events in HCMC is similar to those obtained with rodent mast cells and indicated that the requirement of tyrosine phosphorylation in the activation process of each of the signaling molecules might be different in HCMC and rodent mast cells. Our finding indicates that HCMC may be useful for analysis of Fc epsilonRI-mediated signal transduction in human mast cells.
Microemulsion Approach to Non‐Agglomerated and Crystalline Nanomaterials (2008)

Abstract A wide variety of different nanomaterials, including ZnO, (NH 4 )Y(C 2 O 4 ) 2 ·H 2 O, Y(OH) 3 , Y 2 O 3 , In 2 O 3 :Sn, CePO 4 :Tb, CaCO 3 , CuS, Co 3 [Co(CN) 6 ] 2 , and K 3 [Co(NO 2 ) 6 ] are realized by a microemulsion approach. While heating the micellar system to reflux (200 to 215 °C), highly crystalline materials can be realized, which are still nanosized and do not agglomerate afterwards. Furthermore, a phase separation is initiated by the addition of diethylene glycol, which allows a facile removal of the nanomaterials by the application of low solvent quantitities, and again excludes agglomeration. Both aspects—the realization of highly crystalline materials and the facile separation of non‐agglomerated particles—represent a useful extension of microemulsion techniques. Besides the number and quality of the nanomaterials, the success of the experimental approach is further proven by the realization of physical properties that are restricted to crystalline materials. Namely, these are the bright colors of Co 3 [Co(CN) 6 ] 2 and K 3 [Co(NO 2 ) 6 ] as pigments, the electrical conductivity of In 2 O 3 :Sn (ITO), and the luminescence of CePO 4 :Tb.
Analysis of the LNS implementation of the fast affine projection algorithms (2002)

Abstract-- It has been shown that a 32-bit logarithmic arithmetic unit can operate faster than, and maintain the accuracy of, a 32-bit floating point unit. It uses the logarithmic number system (LNS), in which a real number is represented as a fixedpoint logarithm. In this paper we investigate the implementation of three fast affine projection (FAP) algorithms using LNS. We propose a simplified Conjugate Gradient FAP (SCGFAP) algorithm. We show that the 32-bit or 20-bit LNS implementation of the CGFAP and the SCGFAP algorithm are superior to those of the classical FAP algorithm.
A Novel Class of Nonpeptidic Biaryl Inhibitors of Human Cathepsin K (2003)

A novel series of nonpeptidic biaryl compounds was identified as potent and reversible inhibitors of cathepsin K. The P2-P3 amide bond of a known amino acetonitrile dipeptide 1 was replaced with a phenyl ring, thereby giving rise to this biaryl series that retained potency vs cathepsin K and showed an improved selectivity profile against other cathepsins. Structural modification within this series resulted in the identification of compound (R)-2, a potent human cathepsin K inhibitor (IC(50) = 3 nM) that is selective versus cathepsins B (IC(50) = 3950 nM), L (IC(50) = 3725 nM), and S (IC(50) = 2010 nM). In an in vitro assay involving rabbit osteoclasts and bovine bone, compound (R)-2 inhibited bone resorption with an IC(50) of 95 nM. It was shown that, unlike some peptidic nitrile inhibitors of cysteine proteases, the nitrile moiety of (R)-2 is not converted to the corresponding amide 3 by cathepsin K. This indicates that this class of nonpeptidic nitrile inhibitors is unlikely to be hydrolyzed by cysteine proteases. Furthermore, the inhibition of cathepsin K by compound (R)-2 was shown to be fully reversible and not observably time-dependent. To demonstrate the efficacy of compound (R)-2 in vivo, it was administered to ovariectomized (OVX) rhesus monkeys at 20 mg/kg, po once daily for 8 days, and a urinary marker of bone turnover, N-telopeptide of type I collagen (uNTx), was measured. During the eight-day dosing period, the mean reduction by compound (R)-2 in uNTx was 80% (p < 0.001). This demonstrates that inhibition of cathepsin K leads to an inhibition of this bone resorption marker in OVX rhesus monkeys and strongly suggests that inhibition of cathepsin K is a viable therapeutic approach for the treatment of osteoporosis.
Molecular mechanism underlying the action of a novel fusion inhibitor of influenza A virus (1997)

In the initial stages of influenza virus infection, the hemagglutinin (HA) protein of influenza virus mediates both adsorption and penetration of the virus into the host cell. Recently, we identified and characterized BMY-27709 as an inhibitor of the H1 and H2 subtypes of influenza A virus that specifically inhibits the HA function necessary for virus-cell membrane fusion (G.-X. Luo, R. Colonno, and M. Krystal, Virology 226:66-76, 1996). Studies presented herein show that the inhibition is mediated through specific interaction with the HA protein. This binding represses the low-pH-induced conformational change of the HA protein which is a prerequisite for membrane fusion. In an attempt to define the binding pocket within the HA molecule, a number of drug-resistant viruses have been isolated and characterized. Sequence analyses of the HA gene of these drug-resistant viruses mapped amino acid changes responsible for drug resistance to a region located near the amino terminus of HA2. In addition, we have identified inactive analogs of BMY-27709 which are able to compete out the inhibitory activity of BMY-27709. This finding suggests that inhibition of the HA-mediated membrane fusion by this class of compounds is not solely the result of binding within the HA molecule but requires specific interactions.
Comparison of PCR-RFLP, ribotyping and ERIC-PCR for typingBacillus anthracisandBacillus cereusstrains (2000)

PCR-RFLP analysis of the vrrA gene and cerAB gene was used to investigate the genomic diversity in 21 strains of Bacillus anthracis and 28 strains of Bacillus cereus, and was compared with results obtained by ribotyping and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) analysis. VrrA-typing divided the B. anthracis into four groups. Except for one Pasteur vaccine strain, the vrrA PCR-RFLP profiles of the B. anthracis were separated into three groups, which were different from those of the B. cereus strains. Ribotyping separated the B. anthracis isolates into seven ribotypes, and a common fragment of an approximately 850 bp band from the ERIC-PCR fingerprints separated most B. anthracis strains into two groups. VrrA/cerAB PCR-RFLP, ribotyping and ERIC-PCR generated 18, 22 and 23 types, respectively, from B. cereus strains. The results suggest that a combination of all three methods provides a high resolution typing method for B. anthracis and B. cereus. Compared with ribotyping and ERIC-PCR, PCR-RFLP is simple to perform and has potential as a rapid method for typing and discriminating B. anthracis strains from other B. cereus group bacteria.
Using likely program invariants to detect hardware errors (2008)

In the near future, hardware is expected to become increasingly vulnerable to faults due to continuously decreasing feature size. Software-level symptoms have previously been used to detect permanent hardware faults. However, they can not detect a small fraction of faults, which may lead to Silent Data Corruptions(SDCs). In this paper, we present a system that uses invariants to improve the coverage and latency of existing detection techniques for permanent faults. The basic idea is to use training inputs to create likely invariants based on value ranges of selected program variables and then use them to identify faults at runtime. Likely invariants, however, can have false positives which makes them challenging to use for permanent faults. We use our on-line diagnosis framework for detecting false positives at runtime and limit the number of false positives to keep the associated overhead minimal. Experimental results using microarchitecture level fault injections in full-system simulation show 28.6% reduction in the number of undetected faults and 74.2% reduction in the number of SDCs over existing techniques, with reasonable overhead for checking code.
Experimental Animal Models for Anaerobic Infections (1979)

An experimental animal model that stimulates the mixed aerobic-anaerobic microflora of intraabdominal sepsis was used to study antimicrobial efficacy in vivo. Treatment of infected rats with chloramphenicol resulted in only a modest reduction in the percentage of animals surviving infection with abscesses at necropsy. This unanticipated observation led to further exploration of the predominant anaerobes associated with the experimental infection. In vitro cultures of Bacteroides fragilis, susceptible to chloramphenicol in traditional tests, were capable of reducing chloramphenicol to its aryl amine derivative, which is biologically inactive. In contrast, metronidazole, an antimicrobial agent active against anaerobes, reduced the coli-metronidazole, an antimicrobial agent active against anaerobes, reduced the coli-form-associated mortality in this animal model. Subsequent studies showed that this reduction in mortality is dependent on the presence of an anaerobe and that the levels of Escherichia coli in mixed continuous culture with B. fragilis are reduced by addition of metronidazole. This reduction following addition of metronidazole was not observed either in a pure culture of E. coli or when clindamycin was added to a mixed culture. In a modification of the previously described model, infective material was placed subcutaneously into Wistar rats. Studies with this model suggested that the host's response to bacterial challenge is dependent on the site of infection and that organotropism of the implanted bacterial species is an important determinant of infection.
Experimental Verification of Designer Surface Plasmons (2005)

We studied the microwave reflectivity of a structured, near perfectly conducting substrate that was designed to verify the existence of a theoretically proposed new class of surface mode. Measurements of the mode's dispersion curve show that it correctly approaches the predicted asymptotic frequency; the curve also agrees well with that derived from a computer simulation. Modeling of the field distribution on resonance provides evidence of strong localization of the electric field at the interface and substantial power flow along the interface, thus verifying the surface plasmon-like nature of the mode.