gebhart/patrepeval huggingface dataset:
MUOS radio resource management algorithms (2008)

The mobile user objective system (MUOS) is a next generation military satellite communication system based on the terrestrial 3GPP WCDMA (3rd generation partnership project - wideband code division multiple access) waveform. In any CDMA system, efficient radio resource management (RRM) algorithms are used to assign users to active cells, beams, carriers, and codes to minimize multiple- access-interference (MAI) and maximize system capacity. In MUOS, designing RRM algorithms becomes even more challenging due to very large cell coverage areas, constrained satellite downlink power, differences in uplink and downlink waveforms, and requirements for group services that include point-to-multipoint and netted communications. In this paper, the technical considerations used to develop MUOS RRM algorithms are described. A government provided communication scenario using realistic worldwide user terminal populations is analyzed. The analysis results show that required MUOS system performance can be optimized using two different active carrier plans, each with certain advantages. These approaches together with the tuning of RRM algorithms can be used to optimize MUOS performance for alternative scenarios and for the future growth of the MUOS user terminal and satellite population.
The Efficacy of Intranasal Administration of Dexmedetomidine and Ketamine to Yellow-Bellied Sliders (Trachemys scripta scripta) (2012)

Abstract The purpose of this study was to evaluate the efficacy of dexmedetomidine and ketamine and reversal with atipamezole administered intranasally to Trachemys scripta scripta . Eight healthy adult turtles received 0.2 and 10 mg/kg of dexmedetomidine and ketamine, respectively. Heart rate, respiratory rate, temperature, and sedation (on a scale of 0–4) were measured. Blood was collected 45 min post induction for drug plasma levels from both the subcarapacial and tail veins, followed by intranasal atipamezole administration (0.5 mg/kg). The most frequent sedation scores (2 and 3) provided a level of sedation deep enough to perform a thorough physical exam and minor clinical procedures. All of the turtles became active in 18.9 ± 7 min after atipamezole administration. No adverse effects were observed and all measured cardiopulmonary parameters were within normal limits. Plasma levels of both dexmedetomidine and ketamine indicated adequate absorption and distribution with no difference in the levels obtained from either the subcarapacial or the tail venipuncture sites. A combination of dexmedetomidine–ketamine can be used intranasally to induce moderate to heavy sedation for physical examination, venipuncture, and other minor procedures in the yellow-bellied slider without adverse effects. In addition, the intranasal administration of atipamezole appears efficacious for reversal of dexmedetomidine. Intranasal administration of anesthetic agents holds promise for sedating and anesthetizing chelonians, which are often difficult to thoroughly examine and restrain for biological sample collection, both in the clinic and in the field.
New serotypes of Treponema hyodysenteriae (1985)

Three isolates of pathogenic Treponema hyodysenteriae, B8044, B6933, and Ack 300/8, were serotyped by double immunodiffusion using lipopolysaccharide antigen and rabbit antiserum. The precipitation reactions of these three isolates were then compared with precipitin reactions from the reference strains of serotypes 1 through 4. The data indicated that B8044, B6933, and Ack 300/8 share antigenic determinants with either serotype 1 or 2 or both. However, when the cross-reacting antibodies were removed by adsorption, all reactions were found to be monospecific. Based on these results, three new serotypes of pathogenic T. hyodysenteriae are proposed: B8044 serotype 5, B6933 serotype 6, and Ack 300/8 serotype 7.
Isolation of Vi Antigen and a Simple Method for Its Measurement (1972)

Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.
Malignant transformation in a nontumorigenic human prostatic epithelial cell line. (2001)

The human prostatic epithelial cell line BPH-1 is normally nontumorigenic in nude mice. The present report demonstrates that this cell line can be permanently transformed by its microenvironment to become tumorigenic. The establishment of a series of tumorigenic sublines based on this parental cell line is described. BPH-1 cells were induced to form tumors either by recombination with human prostatic carcinoma-associated fibroblasts (CAFs) or by exposure to carcinogenic doses of testosterone and estradiol (T+E2) after recombination with rat urogenital sinus mesenchyme. Epithelial cells isolated from these tumors were established as cell strains in culture. When regrafted to nude mouse hosts epithelial cells isolated from CAF- or T+E2-induced tumors were found to be consistently tumorigenic even in the absence of CAF or T+E2. The T+E2-induced cell strains have been designated BPH1(TETD)-A and -B and the CAF-induced strains are designated BPH1(CAFTD)-01 through -08. In vitro, the cells had an epithelial morphology with a less well-defined cobblestone pattern than the parental line. They express SV40 large T antigen, confirming their derivation from the parental BPH-1 line. The BPH1(CAFTD) strains formed colonies in soft agar, whereas the parental BPH-1 cells and the BPH1(TETD) sublines did not. There was no immunocytochemically detectable expression of androgen (AR), alpha-estrogen (ERalpha), or progesterone (PR) receptors by the parental BPH-1 cell line or by any of the tumor-derived cell strains. The cells uniformly coexpressed both basal and luminal cell-type cytokeratins and the basal cell marker p63. When grafted beneath the renal capsule of athymic mouse hosts, all of the tumor-derived cell strains consistently formed tumors. These were predominantly poorly or moderately differentiated squamous or adenosquamous tumors, similar in organization to the primary tumors from which the cell strains were derived. The cell strains continued to express both basal- and luminal-type cytokeratins in vivo. Some of the cell strains also coexpressed vimentin. E-cadherin expression was absent from many of the cells, although patches of cells expressing this marker were seen. The cells continued to express SV40T antigen. These cell strains, which are all derived from a common nontumorigenic progenitor, represent a useful resource for examining genetic and phenotypic changes during carcinogenesis.
Quality of Life Before and After Liver Transplantation for Cholestatic Liver Disease (1999)

Liver transplantation (LT) is an established therapy for patients with end-stage primary biliary cirrhosis (PBC) or primary sclerosing cholangitis (PSC). In this report, we describe the health status and quality of life (QOL) in patients with these cholestatic liver diseases before and after LT. A QOL questionnaire was completed by 157 adult patients with PBC or PSC before and 1 year after liver transplantation at the Mayo Clinic or Baylor University Medical Center. This questionnaire measured four aspects of QOL, including symptoms; physical, social, and emotional functioning; health perceptions; and overall QOL. Changes in these QOL parameters before and after LT were described, and regression analysis was used to assess the relationships between clinical and QOL factors. There were no differences in QOL parameters between patients with PBC and PSC. QOL following transplantation was substantially better than before transplantation. This was observed in all four aspects of QOL. The degree of improvement as measured by effect size (difference in mean scores divided by the pretransplantation standard deviation) was 0.53 for symptoms (P <.01), 1.16 for function (P <.01), 2.37 for health satisfaction (P <.01), and 1.16 for overall QOL (P <.01). Patients' overall QOL before transplantation was significantly related to subjective and objective health status indicators and clinical factors such as ascites and renal dysfunction. QOL at 1-year follow-up, however, could not be adequately predicted by the pretransplantation subjective health status and clinical factors. Patients with end-stage cholestatic disease undergoing LT experience substantial improvement in all aspects of QOL addressed in this study. The patients' QOL 1 year after LT could not be predicted by pretransplantation variables used in this study.
Manipulation of cell-cell and cell-substratum interactions in mouse mammary tumor epithelial cells using broad spectrum antisera. (1981)

Two antisera were raised in goats against material shed by two different mammary epithelial cell lines into serum-free culture medium. These antisera, when added to the medium of intact, growing mouse mammary tumor cells in the absence of complement, cause distinct and dramatic alterations in cell morphology and adhesiveness. One antiserum (anti-SFM I) causes mouse mammary tumor epithelial cells to round and detach from the substratum. Treatment with the other antiserum (anti-SFM II) does not affect cell-substratum interactions, but causes the cells to convert from an epitheloid to a fibroblastic morphology. Statistical analysis of transmission electron micrographs of control and antibody-treated cells indicates that treatment with anti-SFM II is associated with a substantial reduction in the extent of intercellular junctions, particularly desmosomes. To identify the components with which the two antisera interact, nonionic detergent extracts of mouse mammary tumor cells were fractionated, and the ability of various fractions to block the morphological effects of either antiserum was determined. The whole Nonidet P40 (NP40) extract of the epithelial cells blocked the effects of both antisera. After the extract was subjected to ion exchange and lectin affinity chromatography, two separate fractions were obtained. One fraction blocks and anti-SFM I induced rounding and detachment of cells from the substratum. The second fraction blocks the effects of both antisera. The isolation of the former fraction, which has highly restricted number of components, represents a significant first step toward identifying the surface membrane molecule(s) involved in cell-substratum adhesion in epithelial cells.
Magnetism of Fe, Co and Ni nanowires in self-assembled arrays (2001)

Recent work on magnetic properties of transition-metal nanowire arrays produced by electro-deposition is reviewed. The wires, which are electro-deposited into self-assembled porous anodic alumina, form nearly hexagonal arrays characterized by wire diameters down to less than 10 nm, wire lengths up to about 1 µm, and variable centre-to-centre spacings of the order of 50 nm. The fabrication and structural characterization of the arrays is summarized, magnetic data are presented and theoretical explanations of the behaviour of the wires are given. Emphasis is on extrinsic phenomena such as coercivity, magnetization reversal and interactions of the magnetic nanowires. In particular, we analyse how wire imperfections give rise to magnetic localization and dominate the hysteresis behaviour of the wires. Potential applications are outlined in the last section.
BddCut: Towards Scalable Symbolic Cut Enumeration (2007)

While the covering algorithm has been perfected recently by the iterative approaches, such as DAOmap and IMap, its application has been limited to technology mapping. The main factor preventing the covering problem's migration to other logic transformations, such as elimination and resynthesis region identification found in SIS and FBDD, is the exponential number of alternative cuts that have to be evaluated. Traditional methods of cut generation do not scale beyond a cut size of 6. In this paper, a symbolic method that can enumerate all cuts is proposed without any pruning, up to a cut size of 10. We show that it can outperform traditional methods by an order of magnitude and, as a result, scales to 100K gate benchmarks. As a practical driver, the covering problem applied to elimination is shown where it can not only produce competitive area, but also provide more than 6times average runtime reduction of the total runtime in FBDD, a BDD based logic synthesis tool with a reported order of magnitude faster runtime than SIS and commercial tools with negligible impact on area.
Desaturation of linoleic acid from exogenous lipids by isolated chloroplasts (1980)

When [14C]diacylgalactosylglycerol was added to isolated pea or lettuce chloroplasts linolenate synthesis was seen. The desaturation of [14C]linoleate in diacylgalactosylglycerol to [14C]linolenate was stimulated by the addition of a soluble protein fraction containing lipid-exchange activity. Other [14C]acyl lipids were ineffective, except that [14C]phosphatidylcholine in the presence of UDP-galactose and sn-glycerol 3-phosphate could also supply [14C]linoleate for desaturation. These results are consistent with a role of diacylgalactosylglycerol in linolenate synthesis, as indirectly suggested by labelling experiments.
Mode of binding of [3H]dibenzocycloalkenimine (MK‐801) to the N‐methyl‐D‐aspartate (NMDA) receptor and its therapeutic implication (1988)

Binding of the labeled anticonvulsant drug [3H]dibenzocycloalkenimine [(3H]MK-801 to the N-methyl-D-aspartate (NMDA) receptor and its dissociation from the receptor at 25 degrees C are slow processes, both of which follow first order kinetics (t1/2 approximately equal to 70 and 180 min, respectively). Both reactions are markedly accelerated by glutamate and glycine (t1/2 approximately equal to 5-8 and 4 min, respectively), which allow bimolecular association kinetics of the labeled drug with the receptors whereas equilibrium binding of [3H]MK-801 (Kd 2-4 nM) is hardly affected by glutamate and glycine. The data suggest that MK-801 acts as a steric blocker of the NMDA receptor channel. The competitive antagonist D-(-)-2-amino-5-phosphovaleric acid (AP-5) freezes the receptor in a state which precludes either binding of [3H]MK-801 to the receptor channel or its dissociation from it. These findings have therapeutic implications.
Experimental Destruction of Substantia Nigra Initiated by Parkinson Disease Immunoglobulins (1998)

Our data suggest that PD IgG can initiate a relatively specific inflammatory destruction of SN pars compacta neurons in vivo and demonstrate the potential relevance of immune mechanisms in PD.
Influence of short‐term hydrostatic pressure on organization of stress fibers in cultured chondrocytes (1995)

The present study describes changes in the organization of stress fibers that occur in articular cartilage chondrocytes subjected to hydrostatic pressure. Primary cultures of chondrocytes from bovine articular cartilage, grown on coverslips, were subjected to 5, 15, or 30 MPa hydrostatic pressure at 37 degrees C. The pressure was applied continuously or cyclically at two frequencies: 0.125 Hz (4 seconds of pressure and 4 seconds of no pressure) or 0.05 Hz (1 second of pressure and 19 seconds of no pressure) for a period of 2 hours. Control chondrocytes showed a polygonal form with prominent stress fibers extending across the cells. The exposure of cells to 30 MPa pressure caused a nearly total disappearance of stress fibers and retraction of the cells from each other. With pressure at 15 MPa or cyclic pressure, the number of cells with stress fibers was decreased. In cells subjected to 5 MPa pressure, the stress fibers resembled those in control chondrocytes. The pressure effects were reversible after 2 hours. Pressure had no effect on the staining pattern of vinculin, which suggests that microfilaments are more vulnerable to pressure than vinculin. The results indicate that cytoskeletal changes may be an integral part of the response of chondrocytes to hydrostatic pressure.
All-Optical Analog-to-Digital Conversion Based on Polarization-Differential Interference and Phase Modulation (2007)

A novel approach of an all-optical analog-to-digital converter is proposed and demonstrated. One single-phase modulator and a continuous-wave laser diode are used to quantize the sinusoidal tone analog signal. Software sampling measurements yielded an effective number of bits of 4.1. Benefits of the proposed setup are its simple design, high bandwidth, and stability. The scheme is primarily limited in the optical domain by the speed of the phase modulator and in electronic domain by electrical comparators and logic devices
Donor antibodies to HNA-3a implicated in TRALI reactions prime neutrophils and cause PMN-mediated damage to human pulmonary microvascular endothelial cells in a two-event in vitro model (2006)

Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related mortality. Antibodies to HNA-3a are commonly implicated in TRALI. We hypothesized that HNA-3a antibodies prime neutrophils (PMNs) and cause PMN-mediated cytotoxicity through a two-event pathogenesis. Isolated HNA-3a+ or HNA-3a- PMNs were incubated with plasma containing HNA-3a antibodies implicated in TRALI, and their ability to prime the oxidase was measured. Human pulmonary microvascular endothelial cells (HMVECs) were activated with endotoxin or buffer, HNA-3a+ or HNA-3a- PMNs were added, and the coculture was incubated with plasma+/-antibodies to HNA-3a. PMN-mediated damage was measured by counting viable HMVECs/mm2. Plasma containing HNA-3a antibodies primed the fMLP-activated respiratory burst of HNA-3a+, but not HNA-3a-, PMNs and elicited PMN-mediated damage of LPS-activated HMVECs when HNA-3a+, but not HNA-3a-, PMNs were used. Thus, antibodies to HNA-3a primed PMNs and caused PMN-mediated HMVEC cytotoxicity in a two-event model identical to biologic response modifiers implicated in TRALI.
Surrogate Light Chain Expressing Human Peripheral B Cells Produce Self-reactive Antibodies (2003)

Human B cells that coexpress surrogate and conventional light chains (V-preB+L+) show an unusual heavy and light chain antibody repertoire that display evidence of receptor editing. However, it is unclear whether V-preB+L+ B cells have been silenced by receptor editing or still express autoreactive antibodies. Here we report that 68% of the antibodies expressed by V-preB+L+ B cells are autoreactive. A majority of these autoantibodies are true antinuclear antibodies (ANA), and 50% of the ANAs are also reactive with a diverse group of antigens that include dsDNA, ssDNA, immunoglobulin, insulin, and bacterial lipopolysaccharide. Such antibodies are rarely encountered among conventional B cells. We conclude that V-preB+L+ B cells are a unique subset of normal circulating human B cells that escape central tolerance mechanisms and express self-reactive antibodies including potentially harmful ANAs.
Multiparticle Entanglement of Hot Trapped Ions (1999)

We propose an efficient method to produce multiparticle entangled states of ions in an ion trap for which a wide range of interesting effects and applications have been suggested. Our preparation scheme exploits the collective vibrational motion of the ions, but it works in such a way that this motion need not be fully controlled in the experiment. The ions may, e.g., be in thermal motion and exchange mechanical energy with a surrounding heat bath without detrimental effects on the internal state preparation. Our scheme does not require access to the individual ions in the trap.
Source-to-drain nonuniformly doped channel (NUDC) MOSFET structures for high current drivability and threshold voltage controllability (1992)

The source-to-drain nonuniformly doped channel (NUDC) MOSFET has been investigated to improve the aggravation of the V/sub th/ lowering characteristics and to prevent the degradation of the current drivability. The basic concept is to change the impurity ions to control the threshold voltage, which are doped uniformly along the channel in the conventional channel MOSFET, to a nonuniform profile of concentration. The MOSFET was fabricated by using the oblique rotating ion implantation technique. As a result, the V/sub th/ lowering at 0.4- mu m gate length of the NUDC MOSFET is drastically suppressed both in the linear region and in the saturation region as compared with that of the conventional channel MOSFET. Also, the maximum carrier mobility at 0.4- mu m gate length is improved by about 20.0%. Furthermore, the drain current is increased by about 20.0% at 0.4- mu m gate length.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Structure, Molecular Mechanisms, and Evolutionary Relationships in DNA Topoisomerases (2004)

Topoisomerases are enzymes that use DNA strand scission, manipulation, and rejoining activities to directly modulate DNA topology. These actions provide a powerful means to effect changes in DNA supercoiling levels, and allow some topoisomerases to both unknot and decatenate chromosomes. Since their initial discovery over three decades ago, researchers have amassed a rich store of information on the cellular roles and regulation of topoisomerases, and have delineated general models for their chemical and physical mechanisms. Topoisomerases are now known to be necessary for the survival of cellular organisms and many viruses and are rich clinical targets for anticancer and antimicrobial treatments. In recent years, crystal structures have been obtained for each of the four types of topoisomerases in a number of distinct conformational and substrate-bound states. In addition, sophisticated biophysical methods have been utilized to study details of topoisomerase reaction dynamics and enzymology. A synthesis of these approaches has provided researchers with new physical insights into how topoisomerases employ chemistry and allostery to direct the large-scale molecular motions needed to pass DNA strands through each other.
Protein stabilization via hydrophilization (1988)

This paper experimentally verifies the idea presented earlier that the contact of nonpolar clusters located on the surface of protein molecules with water destabilizes proteins. It is demonstrated that protein stabilization can be achieved by artificial hydrophilization of the surface area of protein globules by chemical modification. Two experimental systems are studied for the verification of the hydrophilization approach. The surface tyrosine residues of trypsin are transformed to aminotyrosines using a two-step modification procedure: nitration by tetranitromethane followed by reduction with sodium dithionite. The modified enzyme is much more stable against irreversible thermoinactivation: the stabilizing effect increases with the number of aminotyrosine residues in trypsin and the modified enzyme can become even 100 times more stable than the native one. Alpha-chymotrypsin is covalently modified by treatment with anhydrides or chloroanhydrides of aromatic carboxylic acids. As a result, different numbers of additional carboxylic groups (up to five depending on the structure of the modifying reagent) are introduced into each Lys residue modified. Acylation of all available amino groups of alpha-chymotrypsin by cyclic anhydrides of pyromellitic and mellitic acids results in a substantial hydrophilization of the protein as estimated by partitioning in an aqueous Ficoll-400/Dextran-70 biphasic system. These modified enzyme preparations are extremely stable against irreversible thermal inactivation at elevated temperatures (65-98 degrees C); their thermostability is practically equal to the stability of proteolytic enzymes from extremely thermophilic bacteria, the most stable proteinases known to date.