gebhart/patrepeval huggingface dataset:
Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. (1996)

The activation of plant defensive genes in leaves of tomato plants in response to herbivore damage or mechanical wounding is mediated by a mobile 18-amino acid polypeptide signal called systemin. Systemin is derived from a larger, 200-amino acid precursor called prosystemin, similar to polypeptide hormones and soluble growth factors in animals. Systemin activates a lipid-based signaling cascade, also analogous to signaling systems found in animals. In plants, linolenic acid is released from membranes and is converted to the oxylipins phytodienoic acid and jasmonic acid through the octadecanoid pathway. Plant oxylipins are structural analogs of animal prostaglandins which are derived from arachidonic acid in response to various signals, including polypeptide factors. Constitutive overexpression of the prosystemin gene in transgenic tomato plants resulted in the overproduction of prosystemin and the abnormal release of systemin, conferring a constitutive overproduction of several systemic wound-response proteins (SWRPs). The data indicate that systemin is a master signal for defense against attacking herbivores. The same defensive proteins induced by wounding are synthesized in response to oligosaccharide elicitors that are generated in leaf cells in response to pathogen attacks. Inhibitors of the octadecanoid pathway, and a mutation that interrupts this pathway, block the induction of SWRPs by wounding, systemin, and oligosaccharide elicitors, indicating that the octadecanoid pathway is essential for the activation of defense genes by all of these signals. The tomato mutant line that is functionally deficient in the octadecanoid pathway is highly susceptible to attacks by Manduca sexta larvae. The similarities between the defense signaling pathway in tomato leaves and those of the defense signaling pathways of macrophages and mast cells of animals suggests that both the plant and animal pathways may have evolved from a common ancestral origin.
Proton Nuclear Magnetic Resonance Spectroscopy of Urine and Plasma in Renal Transplantation Follow-Up (1993)

Urine and plasma samples from 39 patients who underwent renal transplantation were analyzed by proton nuclear magnetic resonance (NMR) spectroscopy. The most relevant resonances for evaluating renal function after transplantation were those arising from citrate, trimethylamine-N-oxide (TMAO), alanine, and lactate when compared to creatinine. A resonance at 3.7 ppm was related to cyclosporine toxicity when associated with elevated levels of TMAO. The respective variations in these metabolites in urine could contribute to prognosis and diagnosis of renal function impairment related to cyclosporine toxicity or overdosage, or to rejection. Thus, NMR spectroscopy should improve the follow-up and management of renal transplantation patients.
Composite Spatio-Temporal Event Detection in Multi-Camera Surveillance Networks (2008)

Abstract. In this paper, we present a composite event detection system for multicamera surveillance networks. The proposed framework is able to handle correlations between primitive events that are generated from either a single camera view or multiple camera views with spatial and temporal variations. Composite events are represented in the form of full binary trees, where the leaves nodes represent primitive events, the root node represents the target composite event, and the middle nodes represent defined rules. The multi-layer design of the composite events provides a great extensibility and flexibility to users in different applications. A standardized XML-style event language is designed to describe the composite events, such that inter-agent communications and event detection module construction are conveniently achieved. In our system, a set of graphical interfaces are developed for users to easily define both primitive and high-level composite events. The proposed system is designed in the distributed form, where different components of the system can be deployed on separate processors and communicating with each other over the network. The capabilities and effectiveness of our system have been demonstrated in several real life applications. 1
Antibiotic treatment of sepsis (1995)

Early, appropriate antibiotic therapy is critical to the treatment of the septicemic patient. The degree of organ dysfunction, underlying medical conditions, and physiologic abnormalities are important prognostic factors but are not important in initial antibiotic selection. Initial empiric therapy should be directed against the resident flora of the organ, which is primarily involved in the infectious process. Blood cultures should be obtained in all patients for the initiation of antibiotic therapy, and methods should be employed for the early detection of septicemia. Other conditions that mimic sepsis, e.g., pseudosepsis, should be ruled out initially to avoid an incorrect diagnosis and unnecessary antibiotic therapy. Monotherapy and fully recommended doses of antimicrobial drugs delivered by the intravenous route as soon as the diagnosis is established remain the cornerstone of therapy in treating the septic patient. Monotherapy with an antibiotic of the appropriate spectrum is more than adequate to treat the great majority of septicemic patients. Double-drug therapy is recommended to treat febrile leukopenic compromised hosts, serious P. aeruginosa infections, and selected cases of intra-abdominal sepsis. At the present time, corticosteroids and mediator therapy have no place in the treatment of the septic patient.
Separate roles for N- and C-termini of the STE4 (beta) subunit of the Saccharomyces cerevisiae G protein in the mediation of the growth arrest. Lack of growth-arresting activity of mammalian beta gamma complexes. (1996)

Mating pheromone signal transduction in Saccharomyces cerevisiae involves a G protein composed to Scg1p (Gpa1p), Ste4p and Ste18p subunits, homologous to the alpha, beta and gamma subunits of mammalian G protein. Growth arrest in G1 phase is activated by the Ste4p/Ste18p complex via a downstream pathway and it is negatively controlled by the Scg1p subunit. Here we explored whether mammalian beta or gamma subunits could functionally substitute for their yeast homologues. While no evidence was obtained for functional replacement of Ste4p and Ste18p, we found that overexpression of Ste18p potentiated the effect of hybrid proteins in which the N terminus of the Ste4p subunit was replaced by that of the mammalian beta. ste4 mutants having deletions in the N terminus showed a decreased activity in signalling to the downstream effector of the pheromone response. This defect was totally cured by overexpression of Ste18p, indicating that the truncated forms of Ste4p have retained their ability to form an active complex with Ste18p. Removal of six amino acids from the C terminus of Ste4p rendered a completely inactive subunit and this defect persisted in hybrids where the C terminus was placed by that of the beta subunit, indicating that the C terminus of Ste4p is essential to trigger the effector of the yeast pheromone response pathway.
[Effects of naloxone, nalorphine and dalargin on the course of shock due to the combination of mechanical trauma and acute blood loss]. (2005)

Acute experiments on 75 adult rabbits were made to study action of intravenous injections of naloxone (0.1 mg/kg), nalorphine (0.5 and 2.0 mg/kg) and dalargin (0.1 mg/kg) in early and late periods of irreversible shock caused by combination of non-shockogenic mechanical trauma of the hip and safe blood loss from the femoral artery. In early shock antagonists of opiate receptors did not influence the outcomes while injection of dalargin prolonged survival of the animals noticeably. In late shock injection of the drugs had no significant influence on the course of the pathological process while reinfusion did not help the animals to survive. In the late shock injection of the above drugs after blood reinfusion increased the rabbits' survival.
A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells (2007)

microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dbeta, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.
Dimethyldioxirane reactions: Rate acceleration due to intramolecular H-bonding (1996)

Absolute rate studies were carried out on a series of C(SINGLE BOND)H insertion reactions of dimethyldioxirane (1a). The substrates were chosen so that the distance between a single tertiary C(SINGLE BOND)H bond and an OH group could be varied. The measured rate constants indicate that a rate acceleration occurs when the distance between the reacting C(SINGLE BOND)H bond and the OH group permits intramolecular H-bonding stabilization of the transition state. A similar study in related compounds without the OH group showed no effect of chain length on the rate of the C(SINGLE BOND)H insertion reaction. A related study of the epoxidation reaction of la also found an increased rate when chain length permitted intramolecular H-bonding by an OH group.
Binding of insect apolipophorin III to dimyristoylphosphatidylcholine vesicles. Evidence for a conformational change. (1994)

Apolipophorin-III (apoLp-III), a hemolymph protein of Manduca sexta, can reversibly associate with the surface of lipoprotein particles. In order to examine the lipid-associated form of apoLp-III, the present studies investigate the structure and properties of apoLp-III complexes with dimyristoylphosphatidylcholine (DMPC). Association of apoLp-III with DMPC vesicles results in the formation of uniform discs with an average diameter and width of 18.5 +/- 2.0 nm and 4.8 +/- 0.8 nm, respectively, as determined by electron microscopy. ApoLp-III.DMPC complexes analyzed by pore-limiting native gradient PAGE demonstrated that a single major species of complex was formed within a wide range of lipid to protein molar ratios (DMPC:apoLp-III; 13:1 to 360:1). Flotation equilibrium experiments, conducted in an analytical ultracentrifuge, confirmed that only one species of apoLp-III.DMPC complex was formed at an initial lipid to protein molar ratio of 67:1, with an apparent molecular mass of 642,000. Complexes cross-linked with dimethyl suberimidate indicate that there are a maximum of 6 apoLp-III molecules per disc. Circular dichroism experiments revealed that apoLp-III becomes essentially completely alpha-helical on formation of apoLp-III.DMPC complexes. Compared to apoLp-III in the lipid-free state, apoLp-III.DMPC complexes were relatively resistant to denaturation by guanidine HCl, displaying denaturation transitions with midpoints at 2.2 and 3.7 M guanidine HCl, respectively. The fluorescence excitation and emission spectra of apoLp-III.DMPC complexes demonstrate a large enhancement of tyrosine fluorescence as compared to the lipid-free state, suggesting that a conformational change occurs when apoLp-III associates with a lipid surface. Denaturation of apoLp-III in the complex by guanidine HCl resulted in a tyrosine fluorescence level similar to that of lipid-free apoLp-III in the presence of guanidine HCl. The tyrosine-induced fluorescence of the complex was quenched with both Cs+ (Kq = 0.573 M-1) and KI (Kq = 0.376 M-1). The results presented in this study indicate that the conformation of apoLp-III is stabilized when complexed with phospholipids and suggest that tyrosine fluorescence provides a sensitive method to detect M. sexta apoLp-III interaction with lipid surfaces.(ABSTRACT TRUNCATED AT 400 WORDS)
Molecular biology of prostate development and prostate cancer (1998)

The molecular mechanisms that control prostate development have been intensely studied in recent years due to the emergence of prostatic cancer as a major health concern. Several recent studies have led to the identification of numerous genes that are required for prostate organogenesis, many of which also contribute to prostate carcinogenesis. These genes fall into several categories, including proto-oncogenes, transcription factors, homeobox genes, growth factors and cell adhesion molecules. This review focuses on those genes which have been implicated in prostate growth and development, and which exhibit deregulated expression in prostate cancer.
The involvement of a conserved family of RNA binding proteins in embryonic development and carcinogenesis (2002)

Vg1 RBP is a member of a family of highly conserved proteins that appear to be involved in RNA localization, stability, and/or translational control in a wide variety of cell types and organisms. Over the last few years, the human homologs of these proteins have been found to be overexpressed in an increasing number of different kinds of cancers. Although the role of these proteins in neoplasia is not understood, results from several labs, including our own, are beginning to suggest that many of these proteins may be important in cell motility, a necessary requirement for metastasis. This paper will review these data and suggest a model for the role of Vg1 RBP and its homologs in embryonic development and carcinogenesis.
CCR2 modulates inflammatory and metabolic effects of high-fat feeding (2005)

The C-C motif chemokine receptor-2 (CCR2) regulates monocyte and macrophage recruitment and is necessary for macrophage-dependent inflammatory responses and the development of atherosclerosis. Although adipose tissue expression and circulating concentrations of CCL2 (also known as MCP1), a high-affinity ligand for CCR2, are elevated in obesity, the role of CCR2 in metabolic disorders, including insulin resistance, hepatic steatosis, and inflammation associated with obesity, has not been studied. To determine what role CCR2 plays in the development of metabolic phenotypes, we studied the effects of Ccr2 genotype on the development of obesity and its associated phenotypes. Genetic deficiency in Ccr2 reduced food intake and attenuated the development of obesity in mice fed a high-fat diet. In obese mice matched for adiposity, Ccr2 deficiency reduced macrophage content and the inflammatory profile of adipose tissue, increased adiponectin expression, ameliorated hepatic steatosis, and improved systemic glucose homeostasis and insulin sensitivity. In mice with established obesity, short-term treatment with a pharmacological antagonist of CCR2 lowered macrophage content of adipose tissue and improved insulin sensitivity without significantly altering body mass or improving hepatic steatosis. These data suggest that CCR2 influences the development of obesity and associated adipose tissue inflammation and systemic insulin resistance and plays a role in the maintenance of adipose tissue macrophages and insulin resistance once obesity and its metabolic consequences are established.
In Vitro and In Vivo Liposome-Mediated Gene Transfer Leads to Human MDR 1 Expression in Mouse Bone Marrow Progenitor Cells (1996)

The ability to select bone marrow cells (BMC) expressing a selectable gene that confers resistance to anticancer drugs would be useful to protect bone marrow during chemotherapy. The human multidrug resistance (MDR1) gene encodes a 170-kD glycoprotein (P-gp), an ATP-dependent transmembrane efflux pump for many different cytotoxic drugs. In this work, we demonstrate efficient expression of the human MDR1 gene in mouse BMC after transfection with a liposomal delivery system (DLS-liposomes). The human MDR1 cDNA expression plasmid (pHaMDR1/A) was encapsulated in DLS-liposomes and delivered to mouse BMC using two approaches: (i) in vitro transfection of BMC followed by bone marrow transplantation and (ii) in vivo direct systemic gene transfer. After both the in vitro and the in vivo approaches, polymerase chain reaction (PCR) analysis confirmed that the human MDR1 gene was successfully transfected to bone marrow, spleen, and peripheral blood (PB) cells, with the human MDR1 gene detected in BMC for up to 30 days after bone marrow transplantation and 28 days after direct systemic administration. Efflux studies using rhodamine-123 demonstrated function of the MDR1 gene product in the in vitro-transfected BMC. Flow cytometry studies using the human MDR1-specific MRK16 monoclonal antibody confirmed the presence of P-gp in BMC after in vitro transfection, as well as in BMC from reconstituted or in vivo-transfected mice. Transgene expression in both lymphoid and myeloid subpopulations of BMC was demonstrated. Colony-forming units (CFU-Mix) were obtained after exposure of BMC to lethal doses of vincristine, demonstrating functional expression of the MDR1 gene in hematopoietic progenitor cells for up to 1 month.
The rapid identification of drug metabolites using capillary liquid chromatography coupled to an ion trap mass spectrometer (1999)

Capillary liquid chromatography (LC) using a 320 microns column and a flow rate of 10 microL/min has been coupled to an ion trap mass spectrometer using electrospray ionisation (ESI) to enable the rapid and effective identification of metabolites in urine, following oral administration of a novel human neutrophil elastase inhibitor, GW311616. Metabolites were identified from their mass (MS) spectra and tandem (MS/MS) mass spectra using minimal sample (1 microL of urine) and no sample pretreatment. Sensitivity assessment has shown that both molecular weight and structural information is obtainable on as little as 5 pg of compound, making the capillary LC/ion trap system as described an ideal analytical tool for the detection and characterisation of low level metabolites in biofluids (particularly when sample volume is limited). This level of detection was unattainable using a triple quadrupole mass spectrometer operating in full-scan mode, although 200 fg on column was detected using selected reaction monitoring target analysis.
Expression of tag‐72 in normal colon, transitional mucosa, and colon cancer (1989)

Monoclonal antibody (MAb) B72.3 detects an epitope carried by high-molecular-weight mucins (tumor-associated glycoprotein, TAG-72) recently identified as sialyl-Tn. B72.3 MAb has a restricted pattern of reactivity with normal tissues but it reacts with a high proportion of epithelial cancers. To determine the possible relationship between neoplastic transformation and reactivity with B72.3 MAb, we have analyzed samples of normal colon, colon cancer and transitional mucosa (mucosa adjacent to colorectal cancer) or reactive mucosa (mucosa adjacent to squamous carcinoma of the anal canal, or mucosa overlying lymphoma). B72.3 MAb reacted strongly with 21/21 specimens of transitional mucosa and with 17/21 specimens of adjacent colon cancer. Reactivity of B72.3 MAb with transitional mucosa was strong and homogeneous, whereas reactivity with cancer tissue was weaker and more heterogeneous. Reactive mucosa adjacent to squamous carcinoma or lymphoma was also reactive with B72.3 MAb. Our findings show that, in the colon, expression of TAG-72 antigen occurs during the process of epithelial cell transformation but is also regulated by factors unrelated to the process of carcinogenesis.
Characteristics of Lipase Modified with Water-soluble Acylating Reagents and Its Esterification Ability (1995)

Lip was modified with various hydrophobic groups, such as Z, Boc, lauroyl, and palmitoyl, using water-soluble acylating reagents. The chemical modification enhanced the dispersibility of Lip in organic solvents, and changed the substrate selectivity, which depends on the length of the carbon chain of triglycerides in hydrolytic reactions. As for the synthesis of triglycerides, Z-modified Lip catalyzed this reaction faster than unmodified Lip. Z-, Boc-, and fatty acid-modified Lips also catalyzed diverse esterification reactions including that of ethyl eicosapentaenoate, lactose oleate, and cholesterol oleate, and the yields of these reactions increased depending on the carbon number of the introducing group.
Use of Semipermeable Polyurethane Membrane for Skin Graft Dressings (1981)

The use of semipermeable polyurethane membrane (Op-site) for skin graft donor-site dressings is now well-accepted. We have used Op-site for skin graft coverage in 25 patients with good results. The dressing modality is not only easy to do, even in anatomically difficult areas, but it also maintains a moist environment, allows daily graft observation, and provides a barrier for exogenous bacteria.
Effects of 2-ME on proliferation, apoptosis and PET-tracer uptake in human prostate cancer cell aggregates (2004)

Effects of 2-ME on proliferation, apoptosis and PET-tracer uptake in human prostate cancer cell aggregates
Chemistry and biology of bile acids (2004)

This review makes an attempt to combine the insights gained into the biochemistry and physiology of bile acids with the elegant supramolecular systems designed from them. Bile acids are cholesterol-derived facial amphiphiles responsible for the solubilization of cholesterol and fat through mixed micelle formation with phospholipids. An intriguing aspect of bile acids is that their chemical structure has been postulated to correlate with vertebrate evolution. However, the etiology of molecular evolution of bile acids is still poorly understood. There has been a steady progress in the studies aimed at elucidating physiological functions and developing pharmacological applications of bile acids. In recent years, bile acids and their analogues have been extensively utilized as supramolecular receptors for various types of guest molecules and ions. Under certain defined conditions, the supramolecular association of bile acids and their derivatives leads to gel formation. Thus, modified bile acids might find use in the design of futuristic materials.
Synthesis of low-leakage PD-SOI circuits with body-biasing (2001)

Article Share on Synthesis of low-leakage PD-SOI circuits with body-biasing Authors: Mario Casu Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, Italy Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, ItalyView Profile , Gianluca Piccinini Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, Italy Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, ItalyView Profile , Guido Masera Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, Italy Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, ItalyView Profile , Maurizio Zamboni Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, Italy Politecnico di Torino, C.so Duca degli Abruzzi, 24, I-10129 Torino, ItalyView Profile Authors Info & Claims ISLPED '01: Proceedings of the 2001 international symposium on Low power electronics and designAugust 2001 Pages 287–290https://doi.org/10.1145/383082.383170Online:06 August 2001Publication History 2citation166DownloadsMetricsTotal Citations2Total Downloads166Last 12 Months5Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Functional interactions in internal translation initiation directed by viral and cellular IRES elements (2001)

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