gebhart/patrepeval huggingface dataset:
Genomic structure and chromosomal mapping of the human CD22 gene (1993)

The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. A nearly full length human CD22 cDNA clone was used to isolate genomic clones that span the CD22 gene. The CD22 gene is spread over 22 kb of DNA and is composed of 15 exons. The first exon contains the major transcriptional start sites. The translation initiation codon is located in exon 3, which also encodes a portion of the signal peptide. Exons 4 to 10 encode the seven Ig domains of CD22, exon 11 encodes the transmembrane domain, exons 12 to 15 encode the intracytoplasmic domain of CD22, and exon 15 also contains the 3' untranslated region. A minor form of CD22 mRNA likely results from splicing of exon 5 to exon 8, skipping exons 6 and 7. A 4.6-kb XbaI fragment of the CD22 gene was used to map the chromosomal location of CD22 by fluorescence in situ hybridization. The hybridization locus was identified by combining fluorescent images of the probe with the chromosomal banding pattern generated by an Alu probe. The results demonstrate that CD22 is located within the band region q13.1 of chromosome 19. Two closely clustered major transcription start sites and several minor start sites were mapped by primer extension. Similarly to many other lymphoid-specific genes, the CD22 promoter lacks an obvious TATA box. Approximately 4 kb of DNA 5' of the transcription start sites were sequenced and found to contain multiple Alu elements. Potential binding sites for the transcriptional factors NF-kappa B, AP-1, and Oct-2 are located within 300 bp 5' of the major transcription start sites. A 400-bp fragment (bp -339 through +71) of the CD22 promoter region was subcloned into a pGEM-chloramphenicol acetyltransferase vector and after transfection into B and T cells was found to be active in both B and T cells. Further studies of the CD22 gene should lead to a greater understanding of the expression of CD22 during B cell development and differentiation.
Synergistic anti-influenza virus A (H1N1) activities of PM-523 (polyoxometalate) and ribavirin in vitro and in vivo (1997)

A Kegin-type polyoxometalate, PM-523, in combination with ribavirin, was tested for its therapeutic effectiveness against influenza virus (FluV) A (H1N1) infection in tissue culture and in mice. PM-523 [(PriNH3)6H [PTi2W10O38(O2)2] x H2O, where Pri is isopropanol] and ribavirin individually inhibited FluV A-induced cytopathic effects in Madin-Darby canine kidney (MDCK) cells at median effective concentrations (EC50s) of 30 and 34 microM, respectively, and at 70% effective concentrations (EC70s) of 48 and 72 microM, respectively. On the other hand, a combination of PM-523 and ribavirin at a ratio of 1:16 exhibited lower EC50s and EC70s than each compound used singly, and combination indices were less than 1. A wide range of combinations of PM-523 and ribavirin at ratios of from 1:128 to 1:1 exhibited additive or synergistic anti-FluV effects in MDCK cells. When these compounds were tested for their anti-FluV A activities in vivo by aerosol exposure of mice which had been infected with a lethal dose of FluV A by an intranasal route, a 1:16 combination of PM-523 and ribavirin was found to have a significantly better therapeutic effect than a single dose of either compound used singly with respect to both the survival rate of the mice and the virus titer in the lungs of the infected mice. PM-523 was effective for the treatment of experimental FluV infection, and in combination with ribavirin, PM-523 exhibited enhanced anti-FluV effects in vitro and in vivo compared with the effect of PM-523 alone.
Myeloperoxidase produces nitrating oxidants in vivo (2002)

Despite intense interest in pathways that generate reactive nitrogen species, the physiologically relevant mechanisms for inflammatory tissue injury remain poorly understood. One possible mediator is myeloperoxidase, a major constituent of neutrophils, monocytes, and some populations of macrophages. The enzyme uses hydrogen peroxide and nitrite to generate 3-nitrotyrosine in vitro. To determine whether myeloperoxidase produces nitrating intermediates in vivo, we used isotope dilution gas chromatography/mass spectrometry to quantify 3-nitrotyrosine in two models of peritoneal inflammation: mice infected with Klebsiella pneumoniae and mice subjected to cecal ligation and puncture. Both models developed an intense neutrophil inflammatory response, and the inflammatory fluid contained markedly elevated levels of 3-chlorotyrosine, a marker of myeloperoxidase action. In striking contrast, 3-nitrotyrosine levels rose only in the mice infected with K. pneumoniae. Levels of total nitrite and nitrate were 20-fold higher in mice injected with K. pneumoniae than in mice subjected to cecal ligation and puncture. Levels of 3-nitrotyrosine failed to increase in mice infected with K. pneumoniae that lacked functional myeloperoxidase. Our observations provide strong evidence that myeloperoxidase generates reactive nitrogen species in vivo and that it operates in this fashion only when nitrite and nitrate become available.
Evaluating signal processing and multimedia applications on SIMD, VLIW and superscalar architectures (2002)

This paper aims to provide a quantitative understanding of the performance of DSP and multimedia applications on very long instruction word (VLIW), single instruction multiple data (SIMD), and superscalar processors. We evaluate the performance of the VLIW paradigm using Texas Instruments Inc.'s TMS320C62xx processor and the SIMD paradigm using Intel's Pentium II processor (with MMX) on a set of DSP and media benchmarks. Tradeoffs in superscalar performance are evaluated with a combination of measurements on Pentium II and simulation experiments on the SimpleScalar simulator. Our benchmark suite includes kernels (filtering, autocorrelation, and dot product) and applications (audio effects, G.711 speech coding, and speech compression). Optimized assembly libraries and compiler intrinsics were used to create the SIMD and VLIW code. We used the hardware performance counters on the Pentium II and the stand-alone simulator for the C62xx to obtain the execution cycle counts. In comparison to non-SIMD Pentium II performance, the SIMD version exhibits a speedup ranging from 1.0 to 5.5 while the speedup of the VLIW version ranges from 0.63 to 9.0. The benchmarks are seen to contain large amounts of available parallelism, however, most of it is inter-iteration parallelism. Out-of-order execution and branch prediction are observed to be extremely important to exploit such parallelism in media applications.
Expression of Fc gamma RIII on HeLa 229 cells: possible effect on in vitro neutralization of Chlamydia trachomatis (1991)

The neutralizing activities of a murine immunoglobulin G3 (IgG3) monoclonal antibody specific for the major outer membrane protein of Chlamydia trachomatis and its monovalent Fab fragments were studied by using Syrian hamster kidney (HaK) cells and human epithelial (HeLa 229) cells. The intact IgG3 antibody was neutralizing for HaK cells but was nonneutralizing for HeLa cells. In contrast, monovalent Fab antibody fragments neutralized chlamydial infectivity for both HaK and HeLa cells. Immunofluorescence analysis of HeLa 229 cells with a panel of monoclonal antibodies specific to human Fc gamma receptors revealed the expression of cell surface Fc gamma RIII. We propose that Fc gamma RIII may obscure the chlamydia-neutralizing activity of certain IgG isotypes by facilitating the Fc gamma R-mediated entry of chlamydiae into HeLa 229 cells. These findings may help explain the inconsistencies that are commonly observed in results when HeLa 229 cells are used in chlamydia neutralization assays.
An open-systems approach to video on demand (1994)

Open systems will enable video servers and set-tops to provide different services in a variety of environments. Hewlett-Packard is interested in applying the principles of open systems to video on demand (VOD). In particular, the company is developing a technology base that will allow their servers and set-tops to operate in a variety of environments and enable the provision of a variety of services. The impact that the different VOD system elements have on the video server and set-top are examined from a communications standpoint. Opportunities for open or standard interfaces are identified and recommendations are made on what these should be where possible.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
ISCOMs (immunostimulating complexes): The first decade (1996)

A little over a decade ago, novel immunostimulating complexes (ISCOMs) were described. This review examines the position and progress that ISCOM technology has achieved in the fields of vaccine research and medicine over this period. Much of the work on ISCOMs has remained in the area of vaccine research where there is still an urgent need for improved adjuvants to help combat important diseases such as AIDS, malaria and influenza. Currently the only widely licensed adjuvants for human use are the aluminium salts, but with the trend towards highly purified subunit vaccines, which are inherently less immunogenic than some of the older vaccines, potent adjuvants capable of promoting specific immune responses are required. ISCOMs are one such technology that offers many of these requirements and as their use in vaccines enters its second decade clinical trials are commencing that will establish whether these submicron, non-living particles composed of saponin, cholesterol, phospholipid and in many cases protein, are useful components for a range of human vaccines.
Dependence Map-Based Flow Control to Reduce Void Content in Liquid Composite Molding (2005)

ABSTRACT Prohibitive costs are preventing liquid composite molding processes, such as resin transfer molding, from gaining greater popularity. The high costs are mainly due to lack of automation and repeatability. Variations inherent in the preform cutting and placement process lead to unpredictable resin infusion patterns that sometimes form regions devoid of resin. This compromises the structural integrity of the composite and the part is rejected as scrap. Control during the filling stage of liquid composite molding processes have been shown to redirect the flow towards regions difficult to impregnate, providing a greater percentage of acceptable manufactured composite parts. However, many of the current techniques have various limitations. Many off-line control approaches depend on anticipation of problems, and on-line approaches are geometrically limited and can be computationally intensive if executed during manufacturing. The present work combines off-line and on-line approaches to infuse and fill ...
Fabrication of monolithic microchannels for IC chip cooling (2005)

A novel method to fabricate microchannels to be used in microelectronics chip cooling has been developed. The channels are made by microelectroplating with thick photoresist as the sacrificial molds. By removing the photoresist mold before sealing is completed, the channel can be made with only one mask. The cross section of the channels presented in the paper are 5-10 pm wide and 8-10 pm high. This method solves the problem of removing the mold from inside the extremely long channels (e.g., length-to-width ratio as large as a thousand). Unlike other methods for fabrication of microchannels, this method can be integrated into existing IC processes and delivers a practical way to cool IC chips with microchannels.
The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution (2008)

Microelectrodes enable localized electrical stimulation and recording, and they have revolutionized our understanding of the spatiotemporal dynamics of systems that generate or respond to electrical signals. However, such comprehensive understanding of systems that rely on molecular signals-e.g., chemical communication in multicellular neural, developmental, or immune systems-remains elusive because of the inability to deliver, capture, and interpret complex chemical information. To overcome this challenge, we developed the "chemistrode," a plug-based microfluidic device that enables stimulation, recording, and analysis of molecular signals with high spatial and temporal resolution. Stimulation with and recording of pulses as short as 50 ms was demonstrated. A pair of chemistrodes fabricated by multilayer soft lithography recorded independent signals from 2 locations separated by 15 mum. Like an electrode, the chemistrode does not need to be built into an experimental system-it is simply brought into contact with a chemical or biological substrate, and, instead of electrical signals, molecular signals are exchanged. Recorded molecular signals can be injected with additional reagents and analyzed off-line by multiple, independent techniques in parallel (e.g., fluorescence correlation spectroscopy, MALDI-MS, and fluorescence microscopy). When recombined, these analyses provide a time-resolved chemical record of a system's response to stimulation. Insulin secretion from a single murine islet of Langerhans was measured at a frequency of 0.67 Hz by using the chemistrode. This article characterizes and tests the physical principles that govern the operation of the chemistrode to enable its application to probing local dynamics of chemically responsive matter in chemistry and biology.
Clinical and Laboratory Features That Differentiate Dengue from Other Febrile Illnesses in an Endemic Area—Puerto Rico, 2007–2008 (2010)

Dengue infection can be challenging to diagnose early in the course of infection before severe manifestations develop, but early diagnosis can improve patient outcomes and promote timely public health interventions. We developed age-based predictive models generated from 2 years of data from an enhanced dengue surveillance system in Puerto Rico. These models were internally validated and were able to differentiate dengue infection from other acute febrile illnesses with moderate accuracy. The accuracy of the models was greater than either the current World Health Organization case definition for dengue fever or a proposed modification to this definition, while requiring the collection of fewer data. In young children, thrombocytopenia and the absence of cough were associated with dengue infection; for adults, rash, leucopenia, and the absence of sore throat were associated with dengue infection; in all age groups, retro-orbital pain was associated with dengue infection.
Design of an RFID-Based Battery-Free Programmable Sensing Platform (2008)

This paper presents the wireless identification and sensing platform (WISP), which is a programmable battery-free sensing and computational platform designed to explore sensor-enhanced radio frequency identification (RFID) applications. WISP uses a 16-bit ultralow-power microcontroller to perform sensing and computation while exclusively operating from harvested RF energy. Sensors that have successfully been integrated into the WISP platform to date include temperature, ambient light, rectified voltage, and orientation. The microcontroller encodes measurements into an electronic product code (EPC) Class 1 Generation 1 compliant ID and dynamically computes the required 16-bit cyclical redundancy checking (CRC). Finally, WISP emulates the EPC protocol to communicate the ID to the RFID reader. To the authors' knowledge, WISP is the first fully programmable computing platform that can operate using power transmitted from a long-range (UHF) RFID reader and communicate arbitrary multibit data in a single response packet.
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis (1981)

We describe the use of gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions. The method, which we call protein distribution analysis, is simple, sensitive and yields thermodynamically rigorous results. It is particularly well suited to studies of simultaneous binding of several proteins to a single nucleic acid. In studies of the lac repressor-operator interaction, we found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites. Pseudo-first order dissociation kinetics of the repressor-203 bp operator complex were found to be temperature sensitive, with delta E of 80 kcal mol-1 above 29 degrees C and 26 kcal mol-1 below. The half life of the complex (5 min at 21 degrees C) is shorter than that reported for very high molecular weight operator-containing DNAs, but longer than values reported for much shorter fragments. The binding of lac repressor core to DNA could not be detected by this technique: the maximum binding constant consistent with this finding is 10(5) M-1.
Closed-loop Identification : Methods, Theory, and Applications (1999)

System identification deals with constructing mathematical models of dynamical systems from measured data. Such models have important applications in many technical and nontechnical areas, such as ...
Highly reactive polyisobutene as a component of a new generation of lubricant and fuel additives (1999)

Abstract This paper describes the synthesis of highly reactive polyisobutene (PIB), a special type of PIB used as a component in the production of lubricant and fuel additives. The reactivity and the chemical structure are described in detail, in relation to the nature of the product and its uses as an additive component.
Steady flow generation in microcirculatory systems (2006)

In this paper we explore the mechanical generation of steady-non pulsatile-flow in microfluidic systems. The rationale of the paper is inspired in the example of cardiovascular systems where at the microscale (i.e. capillaries) the flow is steady rather than pulsatile to optimize performance. We present a solution to the generation of steady flow in engineered microfluidic systems either in open or closed loop configurations via the use of disc pumps. The disc pump consists of a flat rotating disc and utilizes both viscous drag and centrifugal force to achieve pumping. Experiments using single loop and double loop microfluidic systems are presented to characterize the disc pump. Continuous flow generated by the disc pumps can be used to separate particles based on size using recirculating loops and for extraction of small particles without disturbing the concentration of bigger particles. The potential impact of this technology includes sample separation and extraction techniques into portable microfluidic labs-on-a-chip, and long term culture systems for cells in suspension.
Transmission of Mother’s Microflora to the Newborn at Birth (1996)

Our aim was to study the initial microbial colonization of the newborns by comparing it with their mothers' vaginal microflora. Nineteen mother-newborn pairs were examined at delivery. We found a close association, both qualitative and quantitative, between the individually different microflora of a mother's vagina and that of her newborn. The degree of contamination of the newborn significantly correlated with the counts of microorganisms found in the vagina of mothers. In 85% of investigated individual mother-newborn pairs we revealed similar predominant microorganisms. There were no cases of the mothers and their newborns harbouring similar potentially pathogenic prevailing microorganisms.
Selective block of gap junction channel expression with connexin-specific antisense oligodeoxynucleotides (1994)

Gap junctions in vascular smooth muscle provide a cell-to-cell conduction pathway that may contribute to regulation and coordination of changes in vascular tone. A7r5 cells, a cell line derived from embryonic rat aorta, express connexin 43 (Cx43) and connexin 40 (Cx40). Gap junction channels with three distinct unitary conductances (70, 108, and 141 pS) were observed in these cells. Events of each channel type were equally common, with an approximate frequency of 30-35%; however, the frequency at which each channel type was observed in individual cell pairs ranged between 10 and 65%. Treatment of the cells for 24-72 h with an antisense oligodeoxynucleotide (ODN) to Cx43 reduced the relative frequency of the 108- and 141-pS channel events, whereas comparable treatment with antisense Cx40 ODN reduced the frequency at which 70-pS channel events were observed. The simplest explanation of these findings is that Cx43 forms the 108- and 141-pS channels, whereas Cx40 forms the 70-pS channels in A7r5 cells.
“Isolated” Low High-Density Lipoprotein Cholesterol (1997)

An isolated low HDL-C concentration poses a risk for coronary heart disease. The management of this state is controversial. The first step in management is in agreement with experts and includes lifestyle modification (e.g., weight reduction, diet, smoking cessation, aerobic exercise). Estrogen replacement therapy and discontinuance of drugs that secondarily lower HDL-C are additional treatment options. The use of lipid-altering agents has been used in some patients. Nicotinic acid appears to be an effective agent for an isolated low HDL-C. A large clinical trial evaluating the effect of treating an isolated low HDL-C for primary and secondary prevention of coronary events is needed.
Hyaluronidase in human somatic tissues and urine: polymorphism and the activity in diseases. (1995)

The polymorphism of hyaluronidase (EC 3.2.1.35) (Hyase) was studied on a hyaluronan-polyacrylamide gel. Liver, placenta, ovary and breast tissue were found to have 7 active isoforms while leukocytes and platelets 5 and fibroblasts displayed no hyaluronidase activity. In serum, synovial fluid and urine soluble the most acidic forms are present. Desialylation showed that most of the hyaluronidase isoforms differ in the content of sialic acid. In patients with rheumatoid arthritis, hyaluronidase activity in the synovial fluid varied from not detectable to very high. A partial deficiency was demonstrated in sera from some patients with dysostosis multiplex without mucopolysacchariduria. In I-cell disease, hyaluronidase activity in serum was as that in controls.
Demonstration of Functional Similarity of Proposed Biosimilar ABP 501 to Adalimumab (2016)

Similarity in biofunctional activity is a critical component of the totality of evidence required for demonstration of biosimilarity. The functional similarity demonstrated for ABP 501 comprehensively assesses the known mechanisms of action of adalimumab, supporting the conclusion that ABP 501, adalimumab (US), and adalimumab (EU) are likely to be clinically similar.
Gliding arc discharges as a source of intermediate plasma for methane partial oxidation (2005)

Experimental study of intermediate plasma catalyzed partial oxidation of methane is completed and results are presented along with numerical simulations. To establish the best plasma parameters for industrial chemical applications like partial oxidation, the stability mechanism of different plasma sources is reviewed. Various kinds of intermediate plasma sources are discussed for their application to plasma-chemical processes. Diagnostics of gliding arc discharge: electron temperature, gas temperature, current-voltage characteristics, etc. is done. Different configurations of gliding arc such as the gliding arc in tornado and the plasma disc to enhance intermediate plasma properties are presented along with their advantages. Reverse vortex or "tornado" stabilization is used for methane partial oxidation catalysis. For applications where gas velocity is low or gas is stationary, plasma disc can be employed. Analysis of experimental results and comparison with nonplasma system is done to establish clear advantage of intermediate plasma catalysis for the partial oxidation process.