gebhart/patrepeval huggingface dataset:
The Somatogenic Hormones and Insulin-Like Growth Factor-1: Stimulators of Lymphopoiesis and Immune Function (1997)

I. Introduction II. Local GH Axis in Lymphoid Tissue A. Background: extrapituitary production? B. GH expression C. GH regulation D. GHRs E. GHR signaling III. GH Administration A. Effects on the thymus B. Pattern of GH exposure C. GH and immune function in humans IV. PRL A. PRL expression in lymphoid tissues B. PRL receptors C. Administration of PRL, anti-PRL antibodies, or bromocriptine V. Insulin-like Growth Factors A. Background B. IGF peptides C. Regulation of lymphocyte IGF-I D. IGF receptors on lymphocytes E. IGFBPs VI. Actions of IGF-I A. Bone marrow B. Effects on lymphoid organ size C. Effect of in vivo treatment on lymphocyte number and in vitro function D. Functional effects of IGF-I in vivo: antibody responses E. Immune reconstitution F. Mechanism of action: apoptosis VII. IGF-I in Different Physiological States A. IGF-I in pregnancy B. IGF-I in diabetes C. IGF-I in gastrointestinal disorders D. IGF-I action in polycythemia vera VIII. GH/IGF-I as Antistress Hormones IX. Therapeutic Potential X....
Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability (2008)

In vitro-transcribed mRNAs encoding physiologically important proteins have considerable potential for therapeutic applications. However, in its present form, mRNA is unfeasible for clinical use because of its labile and immunogenic nature. Here, we investigated whether incorporation of naturally modified nucleotides into transcripts would confer enhanced biological properties to mRNA. We found that mRNAs containing pseudouridines have a higher translational capacity than unmodified mRNAs when tested in mammalian cells and lysates or administered intravenously into mice at 0.015-0.15 mg/kg doses. The delivered mRNA and the encoded protein could be detected in the spleen at 1, 4, and 24 hours after the injection, where both products were at significantly higher levels when pseudouridine-containing mRNA was administered. Even at higher doses, only the unmodified mRNA was immunogenic, inducing high serum levels of interferon-alpha (IFN-alpha). These findings indicate that nucleoside modification is an effective approach to enhance stability and translational capacity of mRNA while diminishing its immunogenicity in vivo. Improved properties conferred by pseudouridine make such mRNA a promising tool for both gene replacement and vaccination.
Effective treatment of collagen‐induced arthritis by adoptive transfer of CD25+ regulatory T cells (2005)

Our data indicate that CD25+ regulatory T cells can be used for the treatment of systemic, antibody-mediated autoimmune diseases, such as CIA.
Recent advances in polymer shape memory (2011)

Traditional shape memory polymers (SMPs) are those capable of memorizing a temporary shape and recovering to the permanent shape upon heating. Although such a basic concept has been known for half a century, recent progresses have challenged the conventional understanding of the polymer shape memory effect and significantly expanded the practical potential of SMPs. In this article, notable recent advances in the field of SMPs are highlighted. Particular emphasis is placed on how the new developments have changed the conventional view of SMPs, what they mean for practical applications, and where the future opportunities are.
Integrating an aboriginal perspective: issues and challenges faced by non-aboriginal biology teachers (2010)

This exploratory case study investigated the ways non-Aboriginal teachers of Biology conceive of incorporating Aboriginal perspectives into their delivery of the Biology curriculum in Alberta. The participants in this study were non-Aboriginal Biology teachers teaching in schools with predominantly non-Aboriginal students. Semi-structured interviews were conducted with each of the teacher participants and explored issues and challenges that they face infusing Aboriginal contexts into their teaching. The qualitative data generated were coded using themes developed from a conceptual framework for curriculum implementation. The majority of the teachers saw value in incorporating Aboriginal perspectives but shared concerns due to: unclear definitions of Aboriginal and Aboriginal perspectives; an inadequate knowledge base; and lack of material resources and professional development opportunities. Recommendations to help non-Aboriginal teachers include: better access to and targeted professional development and resources; greater clarification on the definitions of Aboriginal and Aboriginal perspectives; and greater amounts of administrative and governmental support.
Ena/VASP Proteins Can Regulate Distinct Modes of Actin Organization at Cadherin-adhesive Contacts (2005)

Functional interactions between classical cadherins and the actin cytoskeleton involve diverse actin activities, including filament nucleation, cross-linking, and bundling. In this report, we explored the capacity of Ena/VASP proteins to regulate the actin cytoskeleton at cadherin-adhesive contacts. We extended the observation that Ena/vasodilator-stimulated phosphoprotein (VASP) proteins localize at cell-cell contacts to demonstrate that E-cadherin homophilic ligation is sufficient to recruit Mena to adhesion sites. Ena/VASP activity was necessary both for F-actin accumulation and assembly at cell-cell contacts. Moreover, we identified two distinct pools of Mena within individual homophilic adhesions that cells made when they adhered to cadherin-coated substrata. These Mena pools localized with Arp2/3-driven cellular protrusions as well as at the tips of cadherin-based actin bundles. Importantly, Ena/VASP activity was necessary for both modes of actin activity to be expressed. Moreover, selective depletion of Ena/VASP proteins from the tips of cadherin-based bundles perturbed the bundles without affecting the protrusive F-actin pool. We propose that Ena/VASP proteins may serve as higher order regulators of the cytoskeleton at cadherin contacts through their ability to modulate distinct modes of actin organization at those contacts.
Gene Based Prediction of Clinically Localized Prostate Cancer Progression After Radical Prostatectomy (2010)

This new gene based classifier has superior predictive power compared to that of the 5-year nomogram to assess the risk of prostate specific antigen recurrence in patients with organ confined prostate cancer. Our classifier should provide more accurate stratification of patients into high and low risk groups for treatment decisions and adjuvant clinical trials.
Convective Assembly and Dry Transfer of Nanoparticles Using Hydrophobic/Hydrophilic Monolayer Templates (2009)

A convective directed-assembly process on a flat substrate that does not require motion and is followed by a dry-transfer process of nanoparticles is presented. The convective assembly process was achieved using Au nanoparticles on hydrophobic/hydrophilic-surface-patterned Si substrates as functions of temperature, gap height, and particle size. An investigation of the particle assembly mechanism showed that the effects of temperature, gap height, and particle size were responsible for controlling the evaporation time, the evaporation length, and the assembly speed, respectively. To ensure conformal contact during the dry-transfer process, chemically patterned hybrid templates with elastic and flexible properties were fabricated and used. The hybrid templates provided conformal contact with a target silicon substrate coated with MPTMS (3-mercaptopropyltrimethoxysilane) and successfully transferred Au particles to the target substrates.
Study on Pudong large-scale development project plan based on detailed district plan in Shanghai city, China (2008)

本稿において、上海市浦東地区を事例として、中国の都市計画における抑制性詳細計画の位置つけとその計画作成と計画審査の特徴を考察した。浦東地区における陸家咀中心区の抑制性詳細計画では、都市デザインの模型により市街地像を提示し、都市デザインのガイドラインを作成した。なお、抑制性詳細計画は土地利用及び建物の計画規制と都市デザイン案を合わせて設けられた土地利用のコントロールの方法であるとわかった。なお、これまでは抑制性詳細計画は権利処分そのものを示すものではなく、計画案は、地元の住民との合意で作成されたものではない。
Fibrillogenesis of β-amyloid (1998)

Conference Article| August 01 1998 Fibrillogenesis of β-amyloid D. Allsop; D. Allsop 1Neurosciences Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex CM19 5AW, U.K. Search for other works by this author on: This Site PubMed Google Scholar D. Howlett; D. Howlett 1Neurosciences Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex CM19 5AW, U.K. Search for other works by this author on: This Site PubMed Google Scholar G. Christie; G. Christie 1Neurosciences Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex CM19 5AW, U.K. Search for other works by this author on: This Site PubMed Google Scholar E. Karran E. Karran 1Neurosciences Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex CM19 5AW, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1998) 26 (3): 459–463. https://doi.org/10.1042/bst0260459 Article history Received: April 16 1998 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation D. Allsop, D. Howlett, G. Christie, E. Karran; Fibrillogenesis of β-amyloid. Biochem Soc Trans 1 August 1998; 26 (3): 459–463. doi: https://doi.org/10.1042/bst0260459 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: Aβ, β-amyloid peptide, APP, amyloid precursor protein, CLIP, chymotrypsin-like activity of proteasome This content is only available as a PDF. © 1998 Biochemical Society1998 Article PDF first page preview Close Modal You do not currently have access to this content.
[Synergic action of the combination of fosfomycin and kanendomycin against gram-positive and gram-negative bacteria]. (1981)

Association between kanendomycin, an aminoglycoside antibiotic with a high antibacterial activity, non susceptible to inactivating action of acetylating enzymes and fosfomycin, an antimicrobial drug active only on bacterial cells, and without toxicity in man, was studied by Authors. An evidence synergism of two examined drugs was found particularly against Proteus spp. and Pseudomonas aeruginosa. A discussion about mechanisms of action of fosfomycin and kanendomycin is referred by Authors to explain this synergism.
Factors influencing endothelial cell proliferation in vitro (1978)

The relative roles of blood cell products and plasma factors on endothelial cell proliferation were evaluated by studying the proliferative response of human umbilical vein endothelial cells to cell free plasma derived serum (CFPDS), whole blood serum (WBS), platelet released factors, fibroblast growth factor and macrophage conditioned medium in vitro. Human adult arterial smooth muscle cells were treated in a similar manner for comparison. The rate of endothelial cell proliferation was directly related to the concentrations of both WBS and CFPDS. Grwoth rate in WBS was marginally greater than that observed in CFPDS during early culture, however, similar confluent densities were achieved. The addition of platelet released factors to CFPDS did not further stimulate endothelial cell proliferation. In contrast smooth muscle cells were quiescent in CFPDS despite increasing serum concentrations, but proliferated actively in response to platelet released factors. Both human macrophage conditioned medium and fibroblast growth factor increased endothelial cell proliferation significantly when compared with CFPDS alone. It is concluded that endothelial cell proliferation in preconfluent cultures is dependent on plasma factors while human vascular smooth muscle cells also require cell derived mitogens such as platelet growth factor to proliferate. The release of a substance by human macrophages mitogenic for endothelial cells may be involved in endothelial cell proliferation in vivo.
Controlled atmosphere electron microscopy (1972)

The use of a gas-reaction stage in an electron microscope extends the range of applications for the instrument and provides a new tool for the study of reaction mechanism in many chemical systems. A JEM 7A high resolution electron microscope has been adapted to incorporate a modified JEOL AGI gas reaction attachment. The technique developed allows for continuous observation of reactions between gases and solids at temperatures up to 1500 K and pressures of up to 30 kN m-2. Continuous recording of the transmission image by a simple video system provides immediate recall and a record which is readily available for subsequent detailed analysis. The limitations and advantages of the technique are critically assessed.
BaO‐TiO 2 ‐WO 3 Microwave Ceramics and Crystalline BaWO 4 (1988)

Microwave ceramic resonators composed of BaO‐TiO 2 ‐WO 3 were developed. The effect of WO 3 addition on the system of BaO·xTiO 2 ·(1+x)yWO 3 (x=4 and 4.5, y=0 to 0.04) was studied. The ceramics of this system are composed of crystallines including Ba 2 Ti 9 O 20 , BaTi 4 O 9 , BaWO 4 , and TiO 2 . At y=0.02, the BaO·4TiO 2 ·0.1WO 3 ceramic was found to have excellent microwave properties such as ε=35, Q=8400 at 6 GHz, and nearly 0 ppm/°C of τ f .
Long-Lasting Enfuvirtide Carrier Pentasaccharide Conjugates with Potent Anti-Human Immunodeficiency Virus Type 1 Activity (2009)

Enfuvirtide (also known as Fuzeon, T-20, or DP-178) is an antiretroviral fusion inhibitor which prevents human immunodeficiency virus type 1 (HIV-1) from entering host cells. This linear 36-mer synthetic peptide is indicated, in combination with other antiretroviral agents, for the treatment of HIV-1-infected individuals and AIDS patients with multidrug-resistant HIV infections. Although enfuvirtide is an efficient anti-HIV-1 drug, its clinical use is limited by a short plasma half-life, i.e., approximately 2 h, which requires twice-daily subcutaneous injections, often resulting in skin sensitivity reaction side effects at the injection sites. Ultimately, 80% of patients stop enfuvirtide treatment within 6 months because of these side effects. We report on the development of long-lasting enfuvirtide conjugates by the use of the site-specific conjugation of enfuvirtide to an antithrombin-binding carrier pentasaccharide (CP) through polyethylene glycol (PEG) linkers of various lengths. These conjugates showed consistent and broad anti-HIV-1 activity in the nanomolar range. The coupling of the CP to enfuvirtide only moderately affected the in vitro anti-HIV-1 activity in the presence of antithrombin. Most importantly, one of these conjugates, enfuvirtide-PEG(12)-CP (EP40111), exhibited a prolonged elimination half-life of more than 10 h in rat plasma compared to the half-life of native enfuvirtide, which was 2.8 h. On the basis of the pharmacokinetic properties of antithrombin-binding pentasaccharides, the anticipated half-life of EP40111 in humans would putatively be about 120 h, which would allow subcutaneous injection once a week instead of twice daily. In conclusion, EP40111 is a promising compound with strong potency as a novel long-lasting anti-HIV-1 drug.
Temperature Dependence of the Energetics of Oligonucleotide-Directed Triple-Helix Formation at a Single DNA Site (1994)

The influence of temperature on the energetics of oligonucleotide-directed triple-helix formation has been investigated in mixed valence salt solutions at pH 7.0. Equilibrium constants for formation of the local pyrimidine.purine.pyrimidine structure afforded by binding of the oligonucleotide 5'-d(T*TTTTCTCTCTCTCT)-3' to a single 15-bp site within a 339-bp plasmid fragment were measured using quantitative affinity cleavage titrations between 8 and 37 °C. In three different solutions buffered by 10 mM Bis-Tris at pH 7.0, BTNS 100 mM NaCl and 1 mM spermine tetrahydrochloride (SpmCl_4)], BTP1 (10 mM NaCl, 140 mM KCl, 1 mM MgCl_2, and 1 mM SpmCl_4), and BTP4 (10 mM NaCl, 140 mM KCl, 1 mM MgCl_2, and 4 mM SpmCl_4), the equilibrium association constants decreased at least 100-fold (from &gt; 10^7 M^(-1) to ca. 10^5 M^(-1)) as the temperature was increased from 8 to 37 °C. Least squares analysis of van't Hoff plots (In K versus 1/T) of the data revealed that in each solution the tripler is enthalpically stabilized by ca. 2 kcal per mol of base triplets. This average value for single-site tripler formation on large DNA at pH 7.0 in mixed valence salt solutions, which reflects contributions from both T•AT and C+GC base triplets, is consistent with those reported previously for a number of oligonucleotide triplexes in solutions containing single cationic species and in excellent agreement with the calorimetrically determined enthalpy for binding of this 15mer to a 21-bp oligonucleotide DNA duplex.
Immunogenicity and antigenicity in rabbits of a repeated sequence of Plasmodium falciparum antigen Pf155/RESA fused to two immunoglobulin G-binding domains of staphylococcal protein A (1990)

A synthetic gene encoding a tetramer of the repeated subunit EENVEHDA of the Plasmodium falciparum antigen Pf155/RESA was expressed in a dual-expression system. The resulting fusion proteins, designated ZZ-M1 and BB-M1, comprised the EENVEHDA repeats and either two immunoglobulin G-binding domains from staphylococcal protein A or the human serum albumin-binding domains from streptococcal protein G, respectively. The soluble fusion proteins were affinity purified to homogeneity in one-step procedures. ZZ-M1 was used for immunization of rabbits. The rabbit antisera reacted with BB-M1 in an enzyme-linked immunosorbent assay and with Pf155/RESA in immunofluorescence of infected erythrocytes and immunoblotting. Inhibition studies revealed that the antibodies mainly recognized epitopes formed by two or more EENVEHDA subunits and were remarkably specific for Pf155/RESA. Importantly, the antibodies also inhibited P. falciparum merozoite reinvasion in vitro efficiently, indicating that they reacted with biologically important epitopes exposed on the native antigen. Immunization with Freund complete adjuvant resulted in high levels of specific immunoglobulin G antibodies over a 1-year period, whereas the antibody response obtained after immunization without adjuvant was generally weaker, immunoglobulin G and M mediated, and not sustained for longer periods. However, these titers were restored after booster injection. Taken together, the results support the usefulness of recombinant gene constructs of this type as immunogens for malaria vaccines.
An architecture for packet-striping protocols (1999)

Link-striping algorithms are often used to overcome transmission bottlenecks in computer networks. Traditional striping algorithms suffer from two major disadvantages. They provide inadequate load sharing in the presence of variable-length packets, and may result in non-FIFO delivery of data. We describe a new family of link-striping algorithms that solves both problems. Our scheme applies to any layer that can provide multiple FIFO channels. We deal with variable-sized packets by showing how fair-queuing algorithms can be transformed into load-sharing algorithms. Our transformation results in practical load-sharing protocols, and shows a theoretical connection between two seemingly different problems. The same transformation can be applied to obtain load-sharing protocols for links with different capacities. We deal with the FIFO requirement for two separate cases. If a sequence number can be added to each packet, we show how to speed up packet processing by letting the receiver simulate the sender algorithm. If no header can be added, we show how to provide quasi FIFO delivery. Quasi FIFO is FIFO except during occasional periods of loss of synchronization. We argue that quasi FIFO is adequate for most applications. We also describe a simple technique for speedy restoration of synchronization in the event of loss. We develop an architectural framework for transparently embedding our protocol at the network level by striping IP packets across multiple physical interfaces. The resulting stripe protocol has been implemented within the NetBSD kernel. Our measurements and simulations show that the protocol offers scalable throughput even when striping is done over dissimilar links, and that the protocol synchronized quickly after packet loss. Measurements show performance improvements over conventional round-robin striping schemes and striping schemes that do not resequence packets. Some aspects of our solution have been implemented in Cisco's router operating system (IOS 11.3) in the context of Multilink PPP striping.
Evidence for tissue-resident mesenchymal stem cells in human adult lung from studies of transplanted allografts (2007)

The origin and turnover of connective tissue cells in adult human organs, including the lung, are not well understood. Here, studies of cells derived from human lung allografts demonstrate the presence of a multipotent mesenchymal cell population, which is locally resident in the human adult lung and has extended life span in vivo. Examination of plastic-adherent cell populations in bronchoalveolar lavage samples obtained from 76 human lung transplant recipients revealed clonal proliferation of fibroblast-like cells in 62% (106 of 172) of samples. Immunophenotyping of these isolated cells demonstrated expression of vimentin and prolyl-4-hydroxylase, indicating a mesenchymal phenotype. Multiparametric flow cytometric analyses revealed expression of cell-surface proteins, CD73, CD90, and CD105, commonly found on mesenchymal stem cells (MSCs). Hematopoietic lineage markers CD14, CD34, and CD45 were absent. Multipotency of these cells was demonstrated by their capacity to differentiate into adipocytes, chondrocytes, and osteocytes. Cytogenetic analysis of cells from 7 sex-mismatched lung transplant recipients harvested up to 11 years after transplant revealed that 97.2% +/- 2.1% expressed the sex genotype of the donor. The presence of MSCs of donor sex identity in lung allografts even years after transplantation provides what we believe to be the first evidence for connective tissue cell progenitors that reside locally within a postnatal, nonhematopoietic organ.
Antigen selection in human lymphomagenesis. (1992)

Although surface immunoglobulin plays a central role in the differentiation and growth of normal B-cells, its role in the growth of human B-cell malignancies is largely a matter of conjecture. Human follicular lymphomas are attractive systems to study in part because they are clones of cells sharing many similarities with germinal center B-cells which are critically dependent on antigen selection for survival. Nucleotide sequence information was determined for the immunoglobulin heavy chain variable genes expressed by two cases of follicular lymphoma. In addition, the germ line variable gene counterparts were also cloned and sequenced from biopsy material obtained from both of these patients. Numerous mutations from germ line were present in the variable genes from both of these cases, many of which accumulated during expansion and growth of these lymphomas. Moreover, the mutations that accumulated during tumor expansion were distributed in a manner that almost certainly was dependent on positive selection presumably mediated by contact with an antigen. These data indicate that antigen selection is probably important for the growth and clonal evolution of follicular lymphomas.
Dissecting the T‐cell response to hordeins in coeliac disease can develop barley with reduced immunotoxicity (2010)

In vivo oral barley challenge offers a convenient and rapid approach to test the immunotoxicity of small amounts of purified hordeins using fresh T-cells from patients in high throughput overnight assays. Barley lines that did not accumulate B- and C-hordeins were viable, yet had substantially reduced immunotoxicity. Creation of hordein-free barley may therefore be possible.