gebhart/patrepeval huggingface dataset:
Hierarchical Multitask Learning With CTC (2018)

In Automatic Speech Recognition, it is still challenging to learn useful intermediate representations when using high-level (or abstract) target units such as words. For that reason, when only a few hundreds of hours of training data are available, character or phoneme-based systems tend to outperform word-based systems. In this paper, we show how Hierarchical Multitask Learning can encourage the formation of useful intermediate representations. We achieve this by performing Connectionist Temporal Classification at different levels of the network with targets of different granularity. Our model thus performs predictions in multiple scales for the same input. On the standard 300h Switchboard training setup, our hierarchical multitask architecture demonstrates improvements over singletask architectures with the same number of parameters. Our model obtains 14.0% Word Error Rate on the Switchboard subset of the Eval2000 test set without any decoder or language model, outperforming the current state-of-the-art on non-autoregressive Acoustic-to-Word models.
The novel mechanism of lenalidomide activity (2015)

Lenalidomide acts by a novel drug mechanism-modulation of the substrate specificity of the CRL4(CRBN) E3 ubiquitin ligase. In multiple myeloma, lenalidomide induces the ubiquitination of IKZF1 and IKZF3 by CRL4(CRBN). Subsequent proteasomal degradation of these transcription factors kills multiple myeloma cells. In del(5q) myelodysplastic syndrome, lenalidomide induces the degradation of CK1α, which preferentially affects del(5q) cells because they express this gene at haploinsufficient levels. In the future, modulation of ubiquitin ligase function may enable us to target previously "undruggable" proteins.
An Electrochemical Gate Based on a Stimuli-Responsive Membrane Associated with an Electrode Surface (2007)

The electrochemical gate based on a chemical signal-responsive membrane was assembled on a Au electrode surface. The polyelectrolyte gel membrane was capable to bind cholesterol because of the hydrogen bonding between cholesterol and the polymer backbone resulting in the gel swelling. The membrane channels were reversibly closed and opened upon addition and washing out cholesterol, respectively. Thus, the electrochemical process of a soluble redox probe, [Fe(CN)(6)](3-/4-), at the membrane-modified electrode was reversibly switched "on-off" by the cyclic addition and washing out cholesterol. The electrochemical reaction was also tuned by the variation of the concentration of the added cholesterol that controlled the extent of the channels closing. The switchable and tuneable operation of the chemically controlled electrochemical gate was characterized by Faradaic impedance spectroscopy and atomic force microscopy, indicating that the extent of the pores opening and closing is controlled by the concentration of the membrane-associated cholesterol. The chemical-responsive electrochemical gate was suggested to be a part of future biochemical/electrochemical systems with logic operations.
Optimization and characterization of tRNA-shRNA expression constructs (2007)

Expression of short hairpin RNAs via the use of PolIII-based transcription systems has proven to be an effective mechanism for triggering RNAi in mammalian cells. The most popular promoters for this purpose are the U6 and H1 promoters since they are easily manipulated for expression of shRNAs with defined start and stop signals. Multiplexing (the use of siRNAs against multiple targets) is one strategy that is being developed by a number of laboratories for the treatment of HIV infection since it increases the likelihood of suppressing the emergence of resistant virus in applications. In this context, the development of alternative small PolIII promoters other than U6 and H1 would be useful. We describe tRNA(Lys3)-shRNA chimeric expression cassettes which produce siRNAs with comparable efficacy and strand selectivity to U6-expressed shRNAs, and show that their activity is consistent with processing by endogenous 3' tRNAse. In addition, our observations suggest general guidelines for expressing effective tRNA-shRNAs with the potential for graded response, to minimize toxicities associated with competition for components of the endogenous RNAi pathway in cells.
Monolayered versus multilayered electroless NiP coatings: Impact of the plating approach on the microstructure, mechanical and corrosion properties of the coatings (2019)

Electroless nickel-phosphorous (NiP) coatings were produced on low carbon steel substrates for a total plating time of 3 h. Different preparation modalities were pursued. Multilayered coatings were produced by stacking three layers of the same composition by successive electroless plating with rinsing steps in between. On the other hand, coatings termed ‘monolayered’ for the sake of comparison were deposited by one step electroless process, with and without undergoing bath replenishment of the electrolyte during plating. All the samples were subjected to thermal annealing at 400 °C for 1 h under argon atmosphere. The results show that the multilayer approach prevents crack propagation in the as-deposited coatings because the interfaces between layers block the advance of defects. Bath replenishment during monolayered coatings production creates pseudo-interfaces similar to those of the multilayered case but they are ineffective in terms of corrosion protection. Un-replenishment of the electrolyte promotes a change in the coating's microstructure from lamellar to columnar which severely worsens their performance. Upon annealing, the presence of interfaces, along with the recrystallization of the metallic matrix, promotes an upgrading of the corrosion performance of the multi-layered coatings. The corrosion products spread laterally at the interface where they stockpile. At a certain point, the accumulation of these by-products provokes the exfoliation of the outermost layer exposing the layer underneath to the corrosive media, thereby delaying the advancement of the corrosion attack. The results of this study highlight the importance of the plating approach selection, as well as the need for proper electrolyte maintenance during the production of high-performance electroless coatings.
Infantile Fibrosarcoma: Management Based on the European Experience (2009)

Although complete resection is rarely feasible at diagnosis, conservative surgery remains the mainstay treatment for infantile fibrosarcoma. An alkylating agent-free and anthracycline-free regimen is usually effective and should be chosen as first-line chemotherapy for inoperable tumors. Overall prognosis is good, but progression or relapse, mainly local, remains possible.
Mass Tag-Assisted Identification of Naturally Processed HLA Class II-Presented Meningococcal Peptides Recognized by CD4+ T Lymphocytes (2005)

The meningococcal class I outer membrane protein porin A plays an important role in the development of T cell-dependent protective immunity against meningococcal serogroup B infection and is therefore a major component of candidate meningococcal vaccines. T cell epitopes from porin A are poorly characterized because of weak in vitro memory T cell responses against purified Ag and strain variation. We applied a novel strategy to identify relevant naturally processed and MHC class II-presented porin A epitopes, based on stable isotope labeling of Ag. Human immature HLA-DR1-positive dendritic cells were used for optimal uptake and MHC class II processing of (14)N- and (15)N-labeled isoforms of the neisserial porin A serosubtype P1.5-2,10 in bacterial outer membrane vesicles. HLA-DR1 bound peptides, obtained after 48 h of Ag processing, contained typical spectral doublets in mass spectrometry that could easily be assigned to four porin A regions, expressed at diverging densities ( approximately 30-4000 copies/per cell). Epitopes from two of these regions are recognized by HLA-DR1-restricted CD4(+) T cell lines and are conserved among different serosubtypes of meningococcal porin A. This mass tag-assisted approach provides a useful methodology for rapid identification of MHC class II presented bacterial CD4(+) T cell epitopes relevant for vaccine development.
METABOLIC COUPLING FACTORS IN PANCREATIC β-CELL SIGNAL TRANSDUCTION (1995)

This chapter focuses on the biochemical mechanisms that mediate glucose-stimulated insulin secretion (GSIS) from beta-cells of the islets of Langerhans and the potentiating role played by fatty acids. We summarize evidence supporting the idea that glucose metabolism is required for GSIS and that the GLUT-2 facilitated glucose transporter and the glucose phosphorylating enzyme glucokinase play important roles in measuring changes in extracellular glucose concentration. The idea that glucose metabolism is linked to insulin secretion through a sequence of events involving changes in ATP:ADP ratio, inhibition of ATP-sensitive K+ channels, and activation of voltage-gated Ca2+ channels is critically reviewed, and the relative importance of ATP generated from glycolytic versus mitochondrial metabolism is evaluated. We also present the growing concept that an important signal for insulin secretion may reside at the linkage between glucose and lipid metabolism, specifically the generation of the regulatory molecule malonyl CoA that promotes fatty acid esterification and inhibits oxidation. Finally, we show that in contrast to its short term potentiating effect on GSIS, long-term exposure of islets to high levels of fatty acids results in beta-cell dysfunction, suggesting that hyperlipidemia associated with obesity may play a causal role in the diminished GSIS characteristic of non insulin-dependent diabetes mellitus (NIDDM).
Synthesis and Pharmacology of α/β3-Peptides Based on the Melanocortin Agonist Ac-His-dPhe-Arg-Trp-NH2 Sequence (2015)

The melanocortin-3 and -4 receptors are expressed in the brain and play key roles in regulating feeding behavior, metabolism, and energy homeostasis. In the present study, incorporation of β(3)-amino acids into a melanocortin tetrapeptide template was investigated. Four linear α/β(3)-hybrid tetrapeptides were designed with the modifications at the Phe, Arg, and Trp residues in the agonist sequence Ac-His-dPhe-Arg-Trp-NH2. The most potent mouse melanocortin-4 receptor (mMC4R) agonist, Ac-His-dPhe-Arg-β(3)hTrp-NH2 (8) showed 35-fold selectivity versus the mMC3R. The study presented here has identified a new template with heterogeneous backbone for designing potent and selective melanocortin receptor ligands.
Two-shot SVBRDF capture for stationary materials (2015)

Material appearance acquisition usually makes a trade-off between acquisition effort and richness of reflectance representation. In this paper, we instead aim for both a light-weight acquisition procedure and a rich reflectance representation simultaneously, by restricting ourselves to one, but very important, class of appearance phenomena: texture-like materials. While such materials' reflectance is generally spatially varying, they exhibit self-similarity in the sense that for any point on the texture there exist many others with similar reflectance properties. We show that the texturedness assumption allows reflectance capture using only two images of a planar sample, taken with and without a headlight flash. Our reconstruction pipeline starts with redistributing reflectance observations across the image, followed by a regularized texture statistics transfer and a non-linear optimization to fit a spatially-varying BRDF (SVBRDF) to the resulting data. The final result describes the material as spatially-varying, diffuse and specular, anisotropic reflectance over a detailed normal map. We validate the method by side-by-side and novel-view comparisons to photographs, comparing normal map resolution to sub-micron ground truth scans, as well as simulated results. Our method is robust enough to use handheld, JPEG-compressed photographs taken with a mobile phone camera and built-in flash.
"There They Go": Short Term Mission Trips as a Metaphor and Model for Youth Slipping out the Back Door (2009)

Millennials slip out the back door of church involvement for a variety of reasons. One currently popular youth ministry activity, Short-Term Mission Trips (STMT), provides a metaphor and a model for this phenomenon. STMT take into account both positive and negative elements as well as a host of factors with intensities that vary from one millennial to another. Utilizing STMT as a metaphor, and the varieties within the model, demonstrate the complexity of the issue rather than providing a singular and simplistic explanation and solution. Inherent in this model is a proactive sending motif rather than a passive clinging for retention.
Sunitinib and other targeted therapies for renal cell carcinoma (2011)

Targeted therapy has radically altered the way metastatic renal cancer is treated. Six drugs are now licensed in this setting, with several other agents under evaluation. Sunitinib is currently the most widely used in the first line setting with impressive efficacy and an established toxicity profile. However, as further randomised studies report and as newer drugs become available this may change. In this review, we address our current understanding of targeted therapy in renal cancer. We also discuss areas in which our knowledge is incomplete, including the identification of correlative biomarkers and mechanisms of drug resistance. Finally, we will describe the major areas of clinical research that will report over the next few years.
Using growth factor conditioning to modify the properties of human cell derived extracellular matrix (2012)

We have recently reported on a bench-top approach for isolating extracellular matrix (ECM) from pure populations of cells grown in culture using sacrificial, open-celled foams to concentrate and capture the ECM. To increase both the accumulation and the strength of the ECM harvested, cell-seeded polyurethane (PU) foams were cultured in media supplemented with either transforming growth factor β-1 (TGFβ1) or hepatocyte growth factor (HGF). At the end of a 3-week culture period, ECM yield was significantly increased for samples conditioned in supplemented media. Control foams yielded 48 ± 12 mg of material for every gram of PU foam seeded. Yield values increased to 102 ± 21 and 243 ± 25 mg for HGF and TGFβ1-treated samples, respectively. HGF supplementation increased the modulus by 59%, while TGFβ1 treatment increased the elastic modulus by 204%. TGFβ1-stimulated material was organized into a network that was markedly denser than control material, with HGF-stimulated network density intermediate to TGFβ1 and controls. Our study showed that TGFβ1-treated samples were collagen enriched while HGF samples had an increased gylcosaminoglycan concentration. The results demonstrate that growth factor supplementation, particularly with TGFβ1, can significantly alter the biomechanical properties of cell-derived ECM that may be used for therapeutic applications.
Answering why and why not questions in user interfaces (2006)

Modern applications such as Microsoft Word have many automatic features and hidden dependencies that are frequently helpful but can be mysterious to both novice and expert users. The ""Crystal"" application framework provides an architecture and interaction techniques that allow programmers to create applications that let the user ask a wide variety of questions about why things did and did not happen, and how to use the related features of the application without using natural language. A user can point to an object or a blank space and get a popup list of questions about it, or the user can ask about recent actions from a temporal list. Parts of a text editor were implemented to show that these techniques are feasible, and a user test suggests that they are helpful and well-liked.
Activation of cyclin D1 and D2 promoters by human T‐cell leukemia virus type I tax protein is associated with IL‐2–independent growth of T cells (2002)

Our aim was to examine the involvement of G(1) cell-cycle regulators in cell growth dysregulation induced by HTLV-I. Compared to uninfected cells, higher expression levels of cyclin D1 and D2 mRNA were detected in HTLV-I-infected T-cell lines, which were at least in part mediated by the viral transforming protein Tax since Tax activated both cyclin D1 and D2 promoters in the human T-cell line Jurkat. A Tax mutant that did not activate NF-kappaB failed to activate cyclin D1 and D2 promoters. Inhibitors of NF-kappaB (dominant negative IkappaBs mutants) suppressed Tax-dependent activation of cyclin D1 and D2 promoters, indicating that Tax-induced activation was mediated by NF-kappaB. Wild-type and mutant Tax capable of activating NF-kappaB, but not Tax mutant incapable of activating NF-kappaB, converted cell growth of a T-cell line from being IL-2-dependent to being IL-2-independent; and this conversion was associated with IL-2-independent induction of cyclins D1 and D2. Our data suggest that induction of cyclins D1 and D2 by Tax is involved in IL-2-independent cell-cycle progression as well as IL-2-independent transformation of primary human T cells by HTLV-I. High expression levels of cyclin D1 and D2 mRNAs were also detected in some patients with ATL. Our findings link HTLV-I infection to changes in cellular D-type cyclin gene expression, transformation of T cells and subsequent development of T-cell leukemia.
Mechanisms of Azole Resistance in Clinical Isolates of Candida glabrata Collected during a Hospital Survey of Antifungal Resistance (2005)

The increasing use of azole antifungals for the treatment of mucosal and systemic Candida glabrata infections has resulted in the selection and/or emergence of resistant strains. The main mechanisms of azole resistance include alterations in the C. glabrata ERG11 gene (CgERG11), which encodes the azole target enzyme, and upregulation of the CgCDR1 and CgCDR2 genes, which encode efflux pumps. In the present study, we evaluated these molecular mechanisms in 29 unmatched clinical isolates of C. glabrata, of which 20 isolates were resistant and 9 were susceptible dose dependent (S-DD) to fluconazole. These isolates were recovered from separate patients during a 3-year hospital survey for antifungal resistance. Four of the 20 fluconazole-resistant isolates were analyzed together with matched susceptible isolates previously taken from the same patients. Twenty other azole-susceptible clinical C. glabrata isolates were included as controls. MIC data for all the fluconazole-resistant isolates revealed extensive cross-resistance to the other azoles tested, i.e., itraconazole, ketoconazole, and voriconazole. Quantitative real-time PCR analyses showed that CgCDR1 and CgCDR2, alone or in combination, were upregulated at high levels in all but two fluconazole-resistant isolates and, to a lesser extent, in the fluconazole-S-DD isolates. In addition, slight increases in the relative level of expression of CgSNQ2 (which encodes an ATP-binding cassette [ABC] transporter and which has not yet been shown to be associated with azole resistance) were seen in some of the 29 isolates studied. Interestingly, the two fluconazole-resistant isolates expressing normal levels of CgCDR1 and CgCDR2 exhibited increased levels of expression of CgSNQ2. Conversely, sequencing of CgERG11 and analysis of its expression showed no mutation or upregulation in any C. glabrata isolate, suggesting that CgERG11 is not involved in azole resistance. When the isolates were grown in the presence of fluconazole, the profiles of expression of all genes, including CgERG11, were not changed or were only minimally changed in the resistant isolates, whereas marked increases in the levels of gene expression, particularly for CgCDR1 and CgCDR2, were observed in either the fluconazole-susceptible or the fluconazole-S-DD isolates. Finally, known ABC transporter inhibitors, such as FK506, were able to reverse the azole resistance of all the isolates. Together, these results provide evidence that the upregulation of the CgCDR1-, CgCDR2-, and CgSNQ2-encoded efflux pumps might explain the azole resistance in our set of isolates.
A 128 K flash EEPROM using double-polysilicon technology (1987)

An easily manufacturable 128 K flash EEPROM (electrically erasable programmable read-only memory) was developed based on a novel cell. Programming is achieved through hot-electron injection and erasing through electron tunneling from the floating gate to the drain. The cell is 20% larger than an EPROM cell and contains an integral series transistor which ensures selflimited erasing, reduces leakage, and increases the cell current. The flash EEPROM device can withstand thousands of program/erase cycles. Endurance failures are due to threshold window closing caused by electron trapping in the gate oxide. Typical erasure time is 1 s to clear the entire memory.
Discover Desktop Conferencing with Netmeeting (1997)

From the Publisher:
As if multimedia Web sites and worldwide e-mail weren't enough, the Internet has blossomed into a great medium for far-flung groups of people to communicate and collaborate with one another. This is possible, thanks to cutting-edge software like Microsoft's NetMeeting.

Now, to make sure you're not left out when the next meeting in cyberspace is called to order, authors Mike Britton and Suzanne Van Cleve offer Discover Desktop Conferencing with NetMeeting 2.0 -- with the latest, ready-to-use versions (not demos or trial versions) of NetMeeting, Chat, and Internet Explorer right on the accompanying bonus CD-ROM. Comprehensive and easy-to-follow even if you're an Internet newbie, Discover Desktop Conferencing with NetMeeting 2.0 shows you step-by-step how to 
Install and customize NetMeeting Make a phone call over the Internet Share an application like Word or Excel with others over the Internet at the same time Transfer files -- to one recipient or many simultaneously Get creative electronically by whiteboarding ideas Have a chat session in which participants type out messages to one another Meet face-to-face even when participants are thousands of miles apart 

Discover Desktop Conferencing with NetMeeting 2.0 also covers how to implement NetMeeting's features in your professional life for virtual meetings, customer service and technical support, telecommuting, and distance learning, as well as in your personal life for staying in touch with friends and family and even making new friends and acquaintances. 
Plus, like all the titles in our Discover series, Discover DesktopConferencing with NetMeeting 2.0 features the 6-Point Advantage: 
Color Tear-Out Card -- Key information and shortcuts that get you up to speed fast Discovery Center -- Quick summaries of the chapters' major points collected at the back of the book Real-Life Vignettes -- Winning stories on how real people use technology to improve their lives Visual Index -- Convenient picture maps direct you to the features you're looking for Web Path -- Handy references to feature- or topic-related Web sites Bonus Section -- Value-added tips and timesaving techniques at the end of each chapter