gebhart/patrepeval huggingface dataset:
Ampullary pyogenic granuloma as a complication of lacrimal plug migration. (2002)

We present a patient with ampullary pyogenic granuloma caused by spontaneous migration of a silicone lacrimal plug. A 43-year-old woman with severely dry eyes was treated with non-absorbable silicone punctal plugs insertion in both superior and inferior puncta. Irritation and purulent discharge gradually developed 3 weeks after implantation. A pink, fleshly ampullary lesion over the left superior punctum was noted later. The migrated silicone plug was found close to the common canaliculus during surgery. Histopathologic examination confirmed the diagnosis of pyogenic granuloma. A migrated lacrimal plug resulting in pyogenic granuloma is a rare complication. The treatment of choice is removal of the migrated plug as early as possible. Patients with lacrimal plugs insertions should be informed of possible complications and should be followed up regularly for early detection of plug-related problems.
The pharmacokinetics of oclacitinib maleate, a Janus kinase inhibitor, in the dog (2013)

The pharmacokinetics of oclacitinib maleate was evaluated in four separate studies. The absolute bioavailability study used a crossover design with 10 dogs. The effect of food on bioavailability was investigated in a crossover study with 18 dogs. The breed effect on pharmacokinetics was assessed in a crossover study in beagles and mongrels dogs. Dose proportionality and multiple dose pharmacokinetics were evaluated in a parallel design study with eight dogs per group. In all four studies, serial blood samples for plasma were collected. Oclacitinib maleate was rapidly and well absorbed following oral administration, with a time to peak plasma concentration of <1 h and an absolute bioavailability of 89%. The prandial state of dogs did not significantly affect the rate or extent of absorption of oclacitinib maleate when dosed orally, as demonstrated by the lack of significant differences in pharmacokinetic parameters between the oral fasted and oral fed treatment groups. The pharmacokinetics of oclacitinib in laboratory populations of beagles and mixed breed dogs also appeared similar. Following oral administration, the exposure of oclacitinib maleate increased dose proportionally from 0.6 to 3.0 mg/kg. Additionally, across the pharmacokinetic studies, there were no apparent differences in oclacitinib pharmacokinetics attributable to sex.
Cytokine Profiles in the Respiratory Tract During Primary Infection With Human Metapneumovirus, Respiratory Syncytial Virus, or Influenza Virus in Infants (2007)

These findings show that viral respiratory infections in infants elicit different cytokine responses and that the pathogeneses of these agents should be studied individually.
Sustainability of Keratinocyte Gene Transfer and Cell Survival In Vivo (1997)

The epidermis is an attractive site for therapeutic gene delivery because it is accessible and capable of delivering polypeptides to the systemic circulation. A number of difficulties, however, have emerged in attempts at cutaneous gene delivery, and central among these is an inability to sustain therapeutic gene production. We have examined two major potential contributing factors, viral vector stamina and involvement of long-lived epidermal progenitor cells. Human keratinocytes were either untreated or transduced with a retroviral vector for beta-galactosidase (beta-Gal) at > 99% efficiency and then grafted onto immunodeficient mice to regenerate human epidermis. Human epidermis was monitored in vivo after grafting for clinical and histologic appearance as well as for gene expression. Although integrated vector sequences persisted unchanged in engineered epidermis at 10 weeks post-grafting, retroviral long terminal repeat (LTR)-driven beta-Gal expression ceased in vivo after approximately 4 weeks. Endogenous cellular promoters, however, maintained consistently normal gene expression levels without evidence of time-dependent decline, as determined by immunostaining with species-specific antibodies for human involucrin, filaggrin, keratinocyte transglutaminase, keratin 10, type VII collagen, and Laminin 5 proteins out to week 14 post-grafting. Transduced human keratinocytes generated multilayer epidermis sustained through multiple epidermal turnover cycles; this epidermis demonstrated retention of a spatially appropriate pattern of basal and suprabasal epidermal marker gene expression. These results confirm previous findings suggesting that viral promoter-driven gene expression is not durable and demonstrate that keratinocytes passaged in vitro can regenerate and sustain normal epidermis for prolonged periods.
Effect of terminal amino acids on the stability and specificity of PNA–DNA hybridisation (2007)

The effect of various charged or hydrophobic amino acids on the hybridisation of fully complementary and mismatch PNA-DNA duplexes was investigated via UV melting curve analysis. The results described here show that the thermal stability and binding specificity of PNA probes can be modified by conjugation to amino acids and these effects should be considered in experimental design when conjugating PNA sequences to solubility enhancing groups or cell transport peptides. Where stabilisation of a duplex is important, without there being a corresponding need for specific binding to fully complementary targets, the conjugation of multiple lysine residues to the C-terminus of PNA may be the best probe design. If, however, the key is to obtain maximum discrimination between fully complementary and mismatch targets, a replacement of glutamic acid for lysine as the routine solubility enhancing group is recommended.
Reflection confocal microscope readout system for three-dimensional photochromic optical data storage (1998)

We propose to use a reflection confocal microscope (RCM) as a readout system for digital data stored in a three-dimensional (3D) photochromic optical memory. We describe the merits and the difficulties for 3D optical memory that are associated with the use of such a RCM.It is shown by means of 3D Fourier space analysis that successful reading of 3D data can be obtained by selection of the appropriate parameter for the RCM.The system parameters include the numerical aperture of the objective lens and the wavelength of the light used for analysis (reading). Experimental results of multilayer recording and reflection confocal reading in photochromic-molecule-doped poly(methyl methacrylate) are presented. Good-contrast images are obtained.
CCR2 Antagonism Alters Brain Macrophage Polarization and Ameliorates Cognitive Dysfunction Induced by Traumatic Brain Injury (2015)

Traumatic brain injury (TBI) is a major risk factor for the development of multiple neurodegenerative diseases. With respect to the increasing prevalence of TBI, new therapeutic strategies are urgently needed that will prevent secondary damage to primarily unaffected tissue. Consistently, neuroinflammation has been implicated as a key mediator of secondary damage following the initial mechanical insult. Following injury, there is uncertainty regarding the role that accumulating CCR2(+) macrophages play in the injury-induced neuroinflammatory sequelae and cognitive dysfunction. Using CX3CR1(GFP/+)CCR2(RFP/+) reporter mice, we show that TBI initiated a temporally restricted accumulation of peripherally derived CCR2(+) macrophages, which were concentrated in the hippocampal formation, a region necessary for learning and memory. Multivariate analysis delineated CCR2(+) macrophages' neuroinflammatory response while identifying a novel therapeutic treatment window. As a proof of concept, targeting CCR2(+) macrophages with CCX872, a novel Phase I CCR2 selective antagonist, significantly reduced TBI-induced inflammatory macrophage accumulation. Concomitantly, there was a significant reduction in multiple proinflammatory and neurotoxic mediators with this treatment paradigm. Importantly, CCR2 antagonism resulted in a sparing of TBI-induced hippocampal-dependent cognitive dysfunction and reduced proinflammatory activation profile 1 month after injury. Thus, therapeutically targeting the CCR2(+) subset of monocytes/macrophages may provide a new avenue of clinical intervention following TBI.
Administration of Growth Hormone to Underweight Patients with Chronic Obstructive Pulmonary Disease (1997)

Patients with chronic obstructive pulmonary disease (COPD) often develop weight loss, which is associated with increased mortality. Recombinant human growth hormone (rhGH) treatment has been proposed to improve nitrogen balance and to increase muscle strength in these patients. The aim of this study was to assess the effects of rhGH administration on the nutritional status, resting metabolism, muscle strength, exercise tolerance, dyspnea, and subjective well-being of underweight patients with stable COPD. Sixteen patients attending a pulmonary rehabilitation program (age: 66 +/- 9 yr; weight: 77 +/- 7% of ideal body weight; FEV1: 39 +/- 13% of predicted) were randomly treated daily with either 0.15 IU/kg rhGH or placebo during 3 wk in a double-blind fashion. Measurements were made at the beginning (DO) and at the end (D21) of treatment and 2 mo later (D81). Body weight was similar in the two groups during the study, but lean body mass was significantly higher in the rhGH group at D21 (p < 0.01) and D81 (p < 0.05). The increase in lean body mass was 2.3 +/- 1.6 kg in the rhGH group and 1.1 +/- 0.9 kg in the control group at D21 and 1.9 +/- 1.6 kg in the rhGH group and 0.7 +/- 2.1 kg in the control group at D81. At D21, the resting energy expenditure was increased in the rhGH group (107.8% of DO, p < 0.001 compared with the control group). At D21 and D81, the changes in maximal respiratory pressures, handgrip strength, maximal exercise capacity, and subjective well-being were similar in the two groups. At D21, the 6-min walking distance decreased in the rhGH group (-13 +/- 31%) and increased in the control group (+10 +/- 14%; p < 0.01). We conclude that the daily administration of 0.15 IU/kg rhGH during 3 wk increases lean body mass but does not improve muscle strength or exercise tolerance in underweight patients with COPD.
ChemInform Abstract: A Synthesis of Pyrrole Derivatives from O‐(2‐Hydroxyethyl)‐ketoximes. (1986)

Abstract Reaction of the oxime (III) with the phosphonium iodide (IV) and subsequent treatment with potassium tert‐butoxide afford 2‐phenylpyrrole (V).
Sequential photobleaching of fluorochromes for polychromatic slide‐based cytometry (2006)

The sequential photobleaching method results in an increased information density on a single cell level and represents an important component to perform polychromatic cytometry.
Psoralens potentiate ultraviolet light-induced inhibition of epidermal growth factor binding. (1986)

The psoralens, when activated by ultraviolet light of 320-400 nm (UVA light), are potent modulators of epidermal cell growth and differentiation. Previously, we reported that, in mammalian cells, these compounds bind to specific saturable high-affinity cellular receptor sites. In the present studies, we demonstrate that binding of psoralens to their receptors followed by UVA light activation is associated with inhibition of epidermal growth factor (EGF) receptor binding. Inhibition of EGF binding, which required UVA light, was rapid and dependent on the dose of UVA light (0.5-2.0 J/cm2), as well as the concentration of psoralens (10 nM to 1 microM). Higher doses of UVA light (2.0-6.0 J/cm2) by themselves were also inhibitory, indicating that psoralens potentiate the UVA-induced inhibition of EGF binding. A number of biologically active analogs of psoralen, including 8-methoxypsoralen, 5-methoxypsoralen, and 4,5',8-trimethylpsoralen, when activated by UVA light, were found to be inhibitors of binding. Inhibition of EGF binding by psoralens was observed in a variety of human and mouse cell culture lines known to possess psoralen receptors. In the epidermal-derived line PAM 212, at least two populations of receptors with different affinities for EGF were found. Psoralens and UVA light selectively inhibited binding to the higher-affinity EGF receptors, an effect analogous to that of the phorbol ester tumor promoters. As observed with phorbol esters, photoactivated psoralens appeared to inhibit EGF binding by an indirect mechanism. These data demonstrate that the psoralens and UVA light have direct biological effects on cell-surface membranes. Since EGF is a growth-regulatory peptide, the ability of psoralens and UVA light to inhibit EGF binding may underlie the biologic effects of these agents in the skin.
Theoretical Basis of Protocols for Seed Storage (1990)

The protocols presently established for optimum seed storage do not account for the chemical composition of different seed species, the physiological status of the seed, and the physical status of water within the seed. The physiological status of seeds from five species with varying chemical compositions was determined by measurements of rates of oxygen uptake and seed deterioration. The physical status of water was determined by water sorption characteristics. For each species studied, there was a specific moisture content for the onset of respiration, chemical reactions, and accelerated aging rates. The moisture contents at which these physiological levels were observed varied among the species and correlated with the lipid content of the seed. However, the changes in physiological activities and the physical status of water occurred at specific relative humidities: 91% for the onset of respiration, 27% for the increased rates of thermal-chemical reactions, and 19% for optimum longevity. Based on these observations, we propose that equilibrating seeds between 19 and 27% relative humidity provides the optimum moisture level for maintaining seed longevity during longterm storage.
Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs (2012)

Photoacoustic tomography (PAT) can create multiscale multicontrast images of living biological structures ranging from organelles to organs. This emerging technology overcomes the high degree of scattering of optical photons in biological tissue by making use of the photoacoustic effect. Light absorption by molecules creates a thermally induced pressure jump that launches ultrasonic waves, which are received by acoustic detectors to form images. Different implementations of PAT allow the spatial resolution to be scaled with the desired imaging depth in tissue while a high depth-to-resolution ratio is maintained. As a rule of thumb, the achievable spatial resolution is on the order of 1/200 of the desired imaging depth, which can reach up to 7 centimeters. PAT provides anatomical, functional, metabolic, molecular, and genetic contrasts of vasculature, hemodynamics, oxygen metabolism, biomarkers, and gene expression. We review the state of the art of PAT for both biological and clinical studies and discuss future prospects.
A New Lesson Plan for Educational Institutions: Expanded Rules Governing Liability Under Title IX of the Education Amendments of 1972 For Student and Faculty Sexual Harassment (2000)

Since Justice Thomas' confirmation hearing in 1991, much public debate and discussion has focused on sexual harassment issues.'Despite this increased public awareness, the incidence of sexual harassment in America's schools 2 is widespread. 3It remains a major barrier to the ability of schools to provide a non-discriminatory, safe learning environment in which students can succeed and achieve their potential.The elimination of sexual harassment in schools is certainly a high priority for students, parents and educators.Despite increased education and public information, a significant number of male and female students experience some form of sexual harassment during their school careers.According to a leading study conducted by the American Association of University Women (AAUW), eighty-five percent of girls and seventy-six percent of boys in grades eight 1. "The nation's sensitivity to sexual harassment has changed profoundly since October 1991, when Anita Hill's charges against then Supreme Court nominee Clarence Thomas riveted Americans to their television sets.Sexual harassment claims filed with the federal government have increased dramatically, as have damages paid to successful plaintiffs."Sarah Glazer, Crackdown on Sexual Harassment: Is the Nation Overreacting to the Problem (July 19, 1996) <http:// libraryip.CQ.com/>.2. As used herein, the term "schools" applies to all public and private educational institutions that receive federal funds, such as elementary and secondary schools, school districts, proprietary schools, colleges and universities.See Sexual Harassment Guidance: Harassment of Students by School Employees, Other Students, or Third Parties, 62 Fed.Reg.12,034 (1997) [hereinafter OCR Guidance].The OCR Guidance provides detailed information regarding standards to identify, prevent, and resolve sexual harassment allegations made by students in educational institutions.As indicated in the Guidance, although such standards do not carry the force of law, "courts generally benefit from, and defer to, the expertise of an agency with authority."Id. at 12,036.See also 20 U.S.C. 1681-1688 (1994) (defining and addressing issues arising from discrimination based on sex or blindness); 34 C.F.R. 106.1-106.71(implementing regulations regarding discrimination in educational programs receiving federal assistance) (1999).3. In a survey of over 200 female students at more than 20 colleges and universities, 67% have felt sexually harassed: 16% by a teacher and 41% by another student.
Physical determining factors of the arterial pulse waveform: theoretical analysis and calculation using the 1-D formulation (2012)

The shape of the arterial pulse waveform is intimately related to the physical properties of the cardiovascular system. It is clinically relevant to measure those properties that are related to cardiovascular function, such as the local elasticity and viscosity of the arterial wall, total compliance and net peripheral resistance of the systemic arterial tree. Most of these properties cannot be directly measured in vivo, but they can be calculated from pressure, flow and wall displacement measurements that can be obtained in vivo. We carry out a linear analysis of the one-dimensional (1-D) equations of blood flow in Voigt-type visco-elastic vessels to study the effects on pulse wave propagation of blood viscosity, flow inertia, wall visco-elasticity, total arterial compliance, net resistance, peripheral outflow pressure, and flow rate at the aortic root. Based on our analysis, we derive methods to calculate the local elastic and viscous moduli of the arterial wall, and the total arterial compliance, net resistance, time constant and peripheral outflow pressure of the systemic arterial tree from pressure, flow and wall displacement data that can be measured in vivo. Analysis of in vivo data is beyond the scope of this study, and therefore, we verify the results of our linear analysis and assess the accuracy of our estimation methods using pulse waveforms simulated in a nonlinear visco-elastic 1-D model of the larger conduit arteries of the upper body, which includes the circle of Willis in the cerebral circulation.
Deregulation of a Homeobox Gene, HOX11, by the t(10;14) in T Cell Leukemia (1991)

Molecular cloning of the t(10;14)(q24;q11) recurrent breakpoint of T cell acute lymphoblastic leukemia has demonstrated a transcript for the candidate gene TCL3. Characterization of this gene from chromosome segment 10q24 revealed it to be a new homeobox, HOX11. The HOX11 homeodomain is most similar to that of the murine gene Hlx and possesses a markedly glycine-rich variable region and an acidic carboxyl terminus. HOX11, while expressed in liver, was not detected in normal thymus or T cells. This lineage-restricted homeobox gene is deregulated upon translocation into the T cell receptor locus where it may act as an oncogene.
Destabilization of apoprotein is insufficient to explain Cu,Zn-superoxide dismutase-linked ALS pathogenesis (2005)

The relative stabilities and structural properties of a representative set of 20 ALS-mutant Cu,Zn-superoxide dismutase apoproteins were examined by using differential scanning calorimetry and hydrogen-deuterium (H/D) exchange followed by MS. Contrary to recent reports from other laboratories, we found that ALS-mutant apoproteins are not universally destabilized by the disease-causing mutations. For example, several of the apoproteins with substitutions at or near the metal binding region (MBR) (MBR mutants) exhibited melting temperatures (Tm) in the range 51.6 degrees C to 56.2 degrees C, i.e., similar to or higher than that of the WT apoprotein (Tm = 52.5 degrees C). The apoproteins with substitutions remote from the MBR (WT-like mutants) showed a wide range of Tms, 40.0 degrees C to 52.4 degrees C. The H/D exchange properties of the mutants were also wide-ranging: the MBR mutant apoproteins exhibited H/D exchange kinetics similar to the WT apoprotein, as did some of the more stable WT-like mutant apoproteins, whereas the less stable apoproteins exhibited significantly less protection from H/D exchange than the WT apoprotein. Most striking were the three mutant apoproteins, D101N, E100K, and N139K, which have apparently normal metallation properties, and differ little from the WT apoprotein in either thermal stability or H/D exchange kinetics. Thus, the ALS mutant Cu,Zn-superoxide dismutase apoproteins do not all share reduced global stability, and additional properties must be identified and understood to explain the toxicity of all of the mutant proteins.
DIABETES AND HYPERTENSION IN A COMMUNITY OF OLDER ADULTS (1981)

The relationship between diabetes and hypertension in 3456 residents of Rancho Bernardo, California, aged 50-79 years, surveyed in 1972-1974, was analyzed in depth in both the univariate mode and after adjustments for the potential confounding effects of age, obesity and diuretic medication. An association between diabetes and hypertension was present in both men and women at all ages, and the association was strongest for subjects having the best evidence for diabetes, i.e., both historical diabetes and fasting hyperglycemia. Adjustment for obesity reduced the association considerably, but a consistent association remained. diabetes and hypertension are linked only partially by obesity. Some of the excess risk of coronary heart disease in diabetes is probably due to hypertension.
Identification of Staphylococcal Nuclease Domain-containing 1 (SND1) as a Metadherin-interacting Protein with Metastasis-promoting Functions (2011)

Metastasis is the deadliest and most poorly understood feature of malignant diseases. Recent work has shown that Metadherin (MTDH) is overexpressed in over 40% of breast cancer patients and promotes metastasis and chemoresistance in experimental models of breast cancer progression. Here we applied mass spectrometry-based screen to identify staphylococcal nuclease domain-containing 1 (SND1) as a candidate MTDH-interacting protein. After confirming the interaction between SND1 and MTDH, we tested the role of SND1 in breast cancer and found that it strongly promotes lung metastasis. SND1 was further shown to promote resistance to apoptosis and to regulate the expression of genes associated with metastasis and chemoresistance. Analyses of breast cancer clinical microarray data indicated that high expression of SND1 in primary tumors is strongly associated with reduced metastasis-free survival in multiple large scale data sets. Thus, we have uncovered SND1 as a novel MTDH-interacting protein and shown that it is a functionally and clinically significant mediator of metastasis.
Segregation Analysis of Esophageal Cancer in 221 High-Risk Chinese Families (1992)

Both an autosomal recessive gene and unexplained environmental factors appear to be important in the etiology of esophageal cancer in the subpopulation studied.