gebhart/patrepeval huggingface dataset:
Retail price response to quality characteristics of fresh peaches by store type (1993)

Information concerning retail price response to quality characteristics is useful when evaluating where fruit with different levels of quality characteristics should be marketed. This article shows how price/quality relationships for produce vary between different types of retail outlets and explores several hypotheses explaining these differences. The results of the analysis confirm the hypothesis that prices at produce markets are more responsive to quality characteristics than prices as supermarkets. Independent markets are responsive to some quality characteristics, time of season, and location, while chain markets show little price response to quality characteristics. © 1993 John Wiley & Sons, Inc.
CD44v6-targeted T cells mediate potent antitumor effects against acute myeloid leukemia and multiple myeloma (2013)

Genetically targeted T cells promise to solve the feasibility and efficacy hurdles of adoptive T-cell therapy for cancer. Selecting a target expressed in multiple-tumor types and that is required for tumor growth would widen disease indications and prevent immune escape caused by the emergence of antigen-loss variants. The adhesive receptor CD44 is broadly expressed in hematologic and epithelial tumors, where it contributes to the cancer stem/initiating phenotype. In this study, silencing of its isoform variant 6 (CD44v6) prevented engraftment of human acute myeloid leukemia (AML) and multiple myeloma (MM) cells in immunocompromised mice. Accordingly, T cells targeted to CD44v6 by means of a chimeric antigen receptor containing a CD28 signaling domain mediated potent antitumor effects against primary AML and MM while sparing normal hematopoietic stem cells and CD44v6-expressing keratinocytes. Importantly, in vitro activation with CD3/CD28 beads and interleukin (IL)-7/IL-15 was required for antitumor efficacy in vivo. Finally, coexpressing a suicide gene enabled fast and efficient pharmacologic ablation of CD44v6-targeted T cells and complete rescue from hyperacute xenogeneic graft-versus-host disease modeling early and generalized toxicity. These results warrant the clinical investigation of suicidal CD44v6-targeted T cells in AML and MM.
Computational models of neural auditory processing (2005)

Explicit neuron firing models are investigated for use in computational modeling of auditory processing. Models for primary auditory neurons are driven by the receptor signal from a hair cell model, which is driven in turn by a filtering model of basilar membrane motion. The output of the primary auditory neuron model is a times-of-firing representation of neural signals. Certain types of processing, such as auto-correlation and cross-correlation, are very simple with this representation, not requiring multiplication. The neuron model used is a leaky-integrate-to-threshold model with a refractory period. Several neurons are modeled at each hair cell, or filter channel. It is found in experiments with these models that the detailed time-of-firing information contains most of the cues of speech formants, pitch, direction, etc. The more conventionally studied firing rate vs. place representation misses important aspects of these cues. Models of pitch perception, binaural directional perception, and sound separation are being based on the cochlear and neural models. The models are all implemented as computational algorithms, and are used in support of related speech recognition and hearing research.
Pharmacokinetics and pharmacodynamics of glucocorticoid suspensions after intra-articular administration (1986)

Triamcinolone acetonide, triamcinolone hexacetonide, and a combination of betamethasone phosphate and acetate were given intra-articularly in different doses. Plasma levels of the steroids were measured and pharmacokinetic parameters were calculated. As a pharmacodynamic parameter for systemic steroid activity, plasma hydrocortisone levels were monitored for 3 weeks. Results indicate complete absorption from the site of injection over a period of 2 to 3 weeks. Because of its lower solubility, triamcinolone hexacetonide is absorbed slower than triamcinolone acetonide, thus maintaining synovial levels for a longer time and creating lower systemic corticoid levels. Endogenous hydrocortisone suppression correlated with exogenous steroid levels. Threshold concentrations for maximum suppression were determined.
Interaction of myelin basic protein with artificial membranes (1993)

The interaction of myelin basic protein (MBP) with large unilamellar vesicles, composed of phosphatidylserine (PtdSer), phosphatidylserine/phosphatidylcholine (PtdSer/Ole2GroPCho) and phosphatidylcholine/cholesterol (Ole2GroPCho/cholesterol) was examined. Binding of MBP to the bilayers as well as the kinetics of this process were determined by a resonance energy transfer procedure. The ability of the protein to aggregate the vesicles subsequently was monitored continuously by absorbance measurements. The interaction was further characterized by determining the ability of MBP to induce membrane perturbations, as reflected by release of aqueous vesicle contents, and lipid mixing. The results demonstrate that Ole2GroPCho inhibits, while PtdSer and cholesterol strongly facilitate MBP-induced membrane aggregation. Furthermore, binding of MBP to vesicles and the subsequent aggregation event are separate processes, i.e. the extent of binding does not necessarily reflect the aggregation susceptibility. Overall, aggregation appears to be the rate-limiting step. Interaction of MBP with PtdSer bilayers results in a limited degree of lipid mixing, which is accompanied by extensive release of vesicle contents. For all other compositions, no lipid mixing occurs, while cholesterol effectively prevents release of vesicle contents. pH-dependent experiments indicate distinct mechanisms to be operative in MBP-induced aggregation of PtdSer and Ole2GroPCho/cholesterol bilayers. At neutral pH, protein-protein interactions appear relevant, while at acidic pH intervesicular bridges, established by monomers that may cause aggregation of PtdSer vesicles, but not of Ole2GroPCho/cholesterol vesicles. The observation that divalent cations reverse MBP-induced vesicle aggregation may have physiological relevance.
Domain Name System Security (DNSSEC) Signing Authority (2000)

This document proposes a revised model of Domain Name System Security (DNSSEC) Signing Authority.The revised model is designed to clarify earlier documents and add additional restrictions to simplify the secure resolution process.
Image wavelet transforms implemented by discrete wavelet chips (1994)

Both discrete wavelet transform (DWT) and inverse DWT are implemented using the lossless quadrature mirror filter (QMF) bank. The image passing through the finite impulse response QMF filtering becomes blurred and thus requires fewer number of pixels. Such a decimation amounts to the critical sampling that leads to the complexity <i>O</i>(<i>N</i>) for <i>N</i> data. The data compression comes from the permissible bits per pixel dynamic range compression of those filtered images having fewer details. The image reconstruction at a telereceiving station is accomplished by means of the inverse DWT. Thus, a complete biorthogonal basis of QMF is implemented by a fast wavelet transform chip designed with Verilog HDL and the image processing is demonstrated numerically. Adaptive DWT is sketched.
Double Cycloisomerization as a Novel and Expeditious Route to Tricyclic Heteroaromatic Compounds: Short and Highly Diastereoselective Synthesis of (.+‐.)‐Tetraponerine T6. (2003)

Cu-Assisted double cycloisomerization of bis-alkynylpyrimidines afforded the 5−6−5 tricyclic heteroaromatic skeleton. This transformation\nwas used as a key step in the highly diastereoselective total synthesis of (±)-tetraponerine T6.
Chick production by in vitro fertilization of the fowl ovum (1994)

The aim of this study was to produce viable chicks by in vitro fertilization and transfer of fertilized ova to the oviduct of recipient hens. Out of a total of 76 transferred ova, 53 were laid with fully calcified shells, 31 of which were fertile (58%). Despite the high rate of embryonic loss, six live chicks were hatched from 12 fertile ova exposed to 0.05 ml of semen (1:200 dilution). Nine healthy chicks were hatched from ten control ova which were recovered from the oviduct following artificial insemination and subsequent transfer to recipient hens. This experimental approach provides a useful model for production of transgenic chicks.
Computational Optogenetics: Empirically-Derived Voltage- and Light-Sensitive Channelrhodopsin-2 Model (2013)

Channelrhodospin-2 (ChR2), a light-sensitive ion channel, and its variants have emerged as new excitatory optogenetic tools not only in neuroscience, but also in other areas, including cardiac electrophysiology. An accurate quantitative model of ChR2 is necessary for in silico prediction of the response to optical stimulation in realistic tissue/organ settings. Such a model can guide the rational design of new ion channel functionality tailored to different cell types/tissues. Focusing on one of the most widely used ChR2 mutants (H134R) with enhanced current, we collected a comprehensive experimental data set of the response of this ion channel to different irradiances and voltages, and used these data to develop a model of ChR2 with empirically-derived voltage- and irradiance- dependence, where parameters were fine-tuned via simulated annealing optimization. This ChR2 model offers: 1) accurate inward rectification in the current-voltage response across irradiances; 2) empirically-derived voltage- and light-dependent kinetics (activation, deactivation and recovery from inactivation); and 3) accurate amplitude and morphology of the response across voltage and irradiance settings. Temperature-scaling factors (Q10) were derived and model kinetics was adjusted to physiological temperatures. Using optical action potential clamp, we experimentally validated model-predicted ChR2 behavior in guinea pig ventricular myocytes. The model was then incorporated in a variety of cardiac myocytes, including human ventricular, atrial and Purkinje cell models. We demonstrate the ability of ChR2 to trigger action potentials in human cardiomyocytes at relatively low light levels, as well as the differential response of these cells to light, with the Purkinje cells being most easily excitable and ventricular cells requiring the highest irradiance at all pulse durations. This new experimentally-validated ChR2 model will facilitate virtual experimentation in neural and cardiac optogenetics at the cell and organ level and provide guidance for the development of in vivo tools.
Notch Signaling in Vascular Development (2003)

Notch signaling is an extremely conserved and widely used mechanism regulating cell fate in metazoans. Interaction of Notch receptors (Notch) with their ligands (Delta-like or Jagged) leads to cleavage of the Notch intracellular domain (NICD) that migrates into the nucleus. In the nucleus, NICD associates with a transcription factor, RBP-Jk. The NICD-RBP-Jk complex, in turn, upregulates expression of primary target genes of Notch signaling, such as hairy and enhancer of split (HES) and HES-related repressor protein (HERP) transcriptional repressors. Recent evidence has demonstrated that the Notch pathway is involved in multiple aspects of vascular development, including proliferation, migration, smooth muscle differentiation, angiogenic processes, and arterial-venous differentiation. In this brief review, we focus on ligands, receptors, and target genes of Notch signaling in the vascular system and discuss (1) tissue distribution; (2) gain- and loss-of-function studies; and (3) the role of Notch components in human diseases involving the vascular system.
Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage (2004)

Abstract Objective To determine the presence of mesenchymal progenitor cells (MPCs) in human articular cartilage. Methods Primary cell cultures established from normal and osteoarthritic (OA) human knee articular cartilage were analyzed for the expression of CD105 and CD166, cell surface markers whose coexpression defines mesenchymal stem cells (MSCs) in bone marrow and perichondrium. The potential of cartilage cells to differentiate to adipogenic, osteogenic, and chondrogenic lineages was analyzed after immunomagnetic selection for CD105+/CD166+ cells and was compared with bone marrow–derived MSCs (BM‐MSCs). Results Up to 95% of isolated cartilage cells were CD105+ and ∼5% were CD166+. The mean ± SEM percentage of CD105+/CD166+ cells in normal cartilage was 3.49 ± 1.93%. Primary cell cultures from OA cartilage contained significantly increased numbers of CD105+/CD166+ cells. Confocal microscopy confirmed the coexpression of both markers in the majority of BM‐MSCs and a subpopulation of cartilage cells. Differentiation to adipocytes occurred in cartilage‐derived cell cultures, as indicated by characteristic cell morphology and oil red O staining of lipid vacuoles. Osteogenesis was observed in isolated CD105+/CD166+ cells as well as in primary chondrocytes cultured in the presence of osteogenic supplements. Purified cartilage‐derived CD105+/CD166+ cells did not express markers of differentiated chondrocytes. However, the cells were capable of chondrocytic differentiation and formed cartilage tissue in micromass pellet cultures. Conclusion These findings indicate that multipotential MPCs are present in adult human articular cartilage and that their frequency is increased in OA cartilage. This observation has implications for understanding the intrinsic repair capacity of articular cartilage and raises the possibility that these progenitor cells might be involved in the pathogenesis of arthritis.
Development of fluorescent tracers for the real-time monitoring of renal function (2011)

Accurate measurement of glomerular filtration rate (GFR) at the bedside is highly desirable in order to assess renal function in real-time, which is currently an unmet clinical need. In our pursuit to develop exogenous fluorescent tracers as GFR markers, various hydrophilic derivatives of 3,6-diaminopyrazine-2,5-dicarboxylic acid with varying molecular weights and absorption/emission characteristics were synthesized. These include polyhydroxyalkyl based small molecules and poly(ethylene glycol) (PEG) substituted moderate molecular weight compounds, which were further sub-grouped into analogs having blue excitation with green emission, and relatively longer wavelength analogs having green excitation with orange emission. Lead compounds were identified in each of the four classes on the basis of structure- activity relationship studies, which included in vitro plasma protein binding, in vivo urine recovery of administered dose, and in vivo optical monitoring. The in vivo optical monitoring experiments with lead candidates have been correlated with plasma pharmacokinetic (PK) data for measurement of clearance and hence GFR. Renal clearance of these compounds, occurring exclusively via glomerular filtration, was established by probenecid blocking experiments. The renal clearance property of all these advanced candidates was superior to that of the iothalamate, which is currently an accepted standard for the measurement of GFR.
DNA Aptamer Folding on Gold Nanoparticles:  From Colloid Chemistry to Biosensors (2008)

We have investigated the effect of the folding of DNA aptamers on the colloidal stability of gold nanoparticles (AuNPs) to which an aptamer is tethered. On the basis of the studies of two different aptamers (adenosine aptamer and K+ aptamer), we discovered a unique colloidal stabilization effect associated with aptamer folding: AuNPs to which folded aptamer structures are attached are more stable toward salt-induced aggregation than those tethered to unfolded aptamers. This colloidal stabilization effect is more significant when a DNA spacer was incorporated between AuNP and the aptamer or when lower aptamer surface graft densities were used. The conformation that aptamers adopt on the surface appears to be a key factor that determines the relative stability of different AuNPs. Dynamic light scattering experiments revealed that the sizes of AuNPs modified with folded aptamers were larger than those of AuNPs modified with unfolded (but largely collapsed) aptamers in salt solution. From both the electrostatic and steric stabilization points of view, the folded aptamers that are more extended from the surface have a higher stabilization effect on AuNP than the unfolded aptamers. On the basis of this unique phenomenon, colorimetric biosensors have been developed for the detection of adenosine, K+, adenosine deaminase, and its inhibitors. Moreover, distinct AuNP aggregation and redispersion stages can be readily operated by controlling aptamer folding and unfolding states with the addition of adenosine and adenosine deaminase.
Dimethylformamide: An Unusual Glycosylation Modulator (2011)

A simple solution: When N,N-dimethylformamide was used to direct the stereochemical course of glycosylation reactions, 1,2-cis glycosylation products were formed with excellent selectivity. A straightforward highly α-stereoselective glycosylation involving preactivation (see scheme) should find broad application and be especially useful for sequential glycosylation reactions to form oligosaccharides. LG=leaving group. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
A Preliminary Pharmacokinetic Study of Intravenous Photofrin ® in Patients (1996)

Photofrin is a light-activated compound used for photodynamic therapy (PDT) of malignant tumors. Although PDT with this drug has been approved for clinical use in the United States, Canada, Japan, and the Netherlands there are few published reports on the biodistribution of Photofrin in humans. In this study we report measurable amounts of Photofrin in human serum up to approximately 1 year following injection of two different Photofrin doses. Concentration-time data were collected from 3, 12, 19, and 10 patients after 0.75, 0.875, 1, and 2 mg Photofrin/kg body weight. Patients who received 2 mg Photofrin/kg were scheduled to undergo intraoperative PDT for the treatment of mesothelioma or carcinoma of the lung. Patients receiving 0.75, 0.875, or 1 mg Photofrin/kg were treated for basal cell carcinoma; 1 mg Photofrin/kg is now a standard dose for PDT of cutaneous malignancies at this institute. For the 1 mg Photofrin/kg dose, a triexponential 3-compartment pharmacokinetic model was fitted to 30 data points pooled from the 19 patients, as if we had one "superpatient." The alpha, beta, and gamma halflives were approximately 16 h, 7.53 days, and 155.56 days, respectively. The mean (+/- SEM) serum concentrations 48 after injection (when most tumors are exposed to drug-activating light) of 0.875, 1, or 2 mg Photofrin/kg were 2.70 +/- 0.47, 4.00 +/- 0.66, and 3.47 +/- 0.97 micrograms Photofrin/ml, respectively. No porphyrin fluorescence could be detected in serum collected from patients 560 to 1335 days after Photofrin injection.
On the crosstalk of reversed- Delta beta directional coupler switches (1988)

Coupled-mode theory applied to reversed- Delta beta parallel directional coupler switches with tapered-sections input and output waveguide predicts a residual crosstalk in the straight through (bar) state due to coupling within the tapered regions which could be as low as 10-15 dB. Complete switching to the cross state, however, can be achieved assuming ideal conditions. Means of reducing the crosstalk in the bar state using phase control are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Features of similarity. (1977)

The metric and dimensional assumptions that underlie the geometric representation of similarity are questioned on both theoretical and empirical grounds. A new set-theoretical approach to similarity is developed in which objects are represented as collections of features, and similarity is described as a feature-matching process. Specifically, a set of qualitative assumptions is shown to imply the contrast model, which expresses the similarity between objects as a linear combination of the measures of their common and distinctive features. Several predictions of the contrast model are tested in studies of similarity with both semantic and perceptual stimuli. The model is used to uncover, analyze, and explain a variety of empirical phenomena such as the role of common and distinctive features, the relations between judgments of similarity and difference, the presence of asymmetric similarities, and the effects of context on judgments of similarity. The contrast model generalizes standard representations of similarity data in terms of clusters and trees. It is also used to analyze the relations of prototypicality and family resemblance
The Noncanonical WNT Pathway Is Operative in Idiopathic Pulmonary Arterial Hypertension (2008)

Idiopathic pulmonary arterial hypertension (IPAH) is a fatal disease that comprises sustained vasoconstriction, enhanced proliferation of pulmonary vascular cells, and in situ thrombosis. The discovery of several contributing signaling pathways in recent years has resulted in an expanding array of novel therapies; however, IPAH remains a progressive disease with poor outcome in most instances. To identify new regulatory pathways of vascular remodeling in IPAH, we performed transcriptome-wide expression profiling of laser-microdissected pulmonary arterial resistance vessels derived from explanted IPAH and nontransplanted donor lung tissues. Statistical analysis of the data derived from six individuals in each group showed significant regulation of several mediators of the canonical and noncanonical WNT pathway. As to the noncanonical WNT pathway, the planar cell polarity (PCP) pathway, the ras homolog gene family member A (RHOA), and ras-related C3 botulinum toxin substrate-1 (RAC1) were strongly up-regulated. Real-time PCR of laser-microdissected pulmonary arteries confirmed these array results and showed in addition significant up-regulation of further PCP mediators wingless member 11 (WNT11), disheveled associated activator of morphogenesis-1 (DAAM1), disheveled (DSV), and RHO-kinase (ROCK). Immunohistochemical staining and semiquantitative expression analysis confirmed the markedly enhanced expression of the PCP mediators in the pulmonary resistance vessels, in particular in the endothelial layer in IPAH. Therefore we propose the PCP pathway to be critically involved in the regulation of vascular remodeling in IPAH.