gebhart/patrepeval huggingface dataset:
Genetic disease detection and DNA amplification using cloned thermostable ligase. (1991)

Polymerase chain reaction, using thermostable DNA polymerase, has revolutionized DNA diagnostics. Another thermostable enzyme, DNA ligase, is harnessed in the assay reported here that both amplifies DNA and discriminates a single-base substitution. This cloned enzyme specifically links two adjacent oligonucleotides when hybridized at 65 degrees C to a complementary target only when the nucleotides are perfectly base-paired at the junction. Oligonucleotide products are exponentially amplified by thermal cycling of the ligation reaction in the presence of a second set of adjacent oligonucleotides, complementary to the first set and the target. A single-base mismatch prevents ligation/amplification and is thus distinguished. This method was exploited to detect 200 target molecules as well as to discriminate between normal beta A- and sickle beta S- globin genotypes from 10-microliters blood samples.
Analysis of pigmented villonodular synovitis with genome‐wide complementary DNA microarray and tissue array technology reveals insight into potential novel therapeutic approaches (2006)

The gene expression signature in PVNS is similar to that of activated macrophages and is consistent with the local destructive course of the disease. The gene and protein expression patterns suggest that the ongoing proliferation in PVNS is sustained by apoptosis resistance. This result suggests the possibility of a potential novel therapeutic intervention against PVNS.
Design of a Pilot SOFC System for the Combined Production of Hydrogen and Electricity under Refueling Station Requirements (2019)

The objective of the current work is to support the design of a pilot hydrogen and electricity producing plant that uses natural gas (or biomethane) as raw material, as a transition option towards a 100% renewable transportation system. The plant, with a solid oxide fuel cell (SOFC) as principal technology, is intended to be the main unit of an electric vehicle station. The refueling station has to work at different operation periods characterized by the hydrogen demand and the electricity needed for supply and self-consumption. The same set of heat exchangers has to satisfy the heating and cooling needs of the different operation periods. In order to optimize the operating variables of the pilot plant and to provide the best heat exchanger network, the applied methodology follows a systematic procedure for multi-objective, i.e. maximum plant efficiency and minimum number of heat exchanger matches, and multi-period optimization. The solving strategy combines process flow modeling in steady state, superstructure-based mathematical programming and the use of an evolutionary-based algorithm for optimization. The results show that the plant can reach a daily weighted efficiency exceeding 60%, up to 80% when considering heat utilization.
A modular transmission line/reluctance head model (1988)

A transmission line model for calculating recording-head fields and efficiencies is described. Exact solutions are obtained for transmission line segments with fixed or varying gap and width. Using these solutions. Thevenin T-reluctance circuit equivalents are obtained for each segment of the total head transmission line structure, and the magnetic flux and fields are obtained by circuit analysis. External fringe fields at edges are also included. Using three basic building blocks: (1) constant-gap, variable or fixed-width regions, (2) graded-gap, variable or fixed-width regions, and (3) external fringe reluctances, in any number and sequence, virtually any head geometry can be quickly and reasonably modeled, without the need for reformulating the problem or respecifying boundary conditions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
N-Methyl-D-Aspartate Receptor (NMDA) Antagonists as Potential Pain Therapeutics (2006)

NMDA receptors are known to be involved in nociceptive transmission and pain processing. Many structurally diverse NMDA antagonists have been reported to have activity in both animal models and clinical models of neuropathic pain. Untoward side effects such as ataxia and sedation have severely limited the clinical uses of this class of potential therapeutics. However, antagonists at the glycine-site, NR2B sites and weak-binding channel blockers have demonstrated an improved side effect profile in animal models of pain. These types of compounds may hold potential promise for future pain therapies. This review covers reported pain data surrounding representative examples of NMDA antagonists and provides a current assessment of potential clinical utility.
Learning interaction for collaborative tasks with probabilistic movement primitives (2014)

This paper proposes a probabilistic framework based on movement primitives for robots that work in collaboration with a human coworker. Since the human coworker can execute a variety of unforeseen tasks a requirement of our system is that the robot assistant must be able to adapt and learn new skills on-demand, without the need of an expert programmer. Thus, this paper leverages on the framework of imitation learning and its application to human-robot interaction using the concept of Interaction Primitives (IPs). We introduce the use of Probabilistic Movement Primitives (ProMPs) to devise an interaction method that both recognizes the action of a human and generates the appropriate movement primitive of the robot assistant. We evaluate our method on experiments using a lightweight arm interacting with a human partner and also using motion capture trajectories of two humans assembling a box. The advantages of ProMPs in relation to the original formulation for interaction are exposed and compared.
Cloning and sequencing of the meso-diaminopimelate-D-dehydrogenase (ddh) gene of Corynebacterium glutamicum. (1988)

The ddh gene of Corynebacterium glutamicum encoding meso-diaminopimelate (meso-DAP)-Ddehydrogenase (DDH) involved in the lysine biosynthesis was cloned in a DAP auxotroph (dap D4) of Escherichia coli by complementation of the DAP auxotroph. Deletion analysis revealed that a - 1.7-kb XhoI-KpnI fragment contained the ddh structure gene. The specific activity of DDH was increased fourteen-fold when C. glutamicum was transformed with a recombinant plasmid harbouring the cloned ddh gene. Furthermore, the ddh gene has been sequenced [S. Ishino et al., Nucleic Acids Res., 15, 3917 (1987)] and some properties of the ddh gene are discussed.
Treatment of Psoriasis With 6-Aminonicotinamide (1975)

Topical applications of a 1.5 percent aqueous solution of 6-aminonicotinamide for four weeks resulted in substantial improvement or complete clearing of plaques of psoriasis in 27 of 34 patients. These results were clearly superior to those that were obtained with the solvent alone. Dermatitis or marked itching occurred in five of 34 patients. Topically applied thionicotinamide also produced improvement or clearing of some psoriatic lesions in an initial screening trial. The toxic hazards of 6-aminonicotinamide to the central nervous system are discussed.
Trichoepithelioma. (2008)

A 29-year-old man presented with a long-standing history of asymptomatic, skin-colored, facial papules and nodules. Histopathologic examination of a representative papule demonstrated trichoepithelioma. The patient had a history of a brother with a similar phenotype, which suggests a diagnosis of familial trichoepithelioma. Linkage and mutational analyses support genetic heterogeneity of familial trichoepithelioma, possibly sharing a clinical spectrum with Brooke-Spiegler syndrome and familial cylindromatosis since each entity has been associated with mutations the CYLD gene.
WT1 Promotes Cell Proliferation in Non-Small Cell Lung Cancer Cell Lines through Up-Regulating Cyclin D1 and p-pRb In Vitro and In Vivo (2013)

The Wilms' tumor suppressor gene (WT1) has been identified as an oncogene in many malignant diseases such as leukaemia, breast cancer, mesothelioma and lung cancer. However, the role of WT1 in non-small-cell lung cancer (NSCLC) carcinogenesis remains unclear. In this study, we compared WT1 mRNA levels in NSCLC tissues with paired corresponding adjacent tissues and identified significantly higher expression in NSCLC specimens. Cell proliferation of three NSCLC cell lines positively correlated with WT1 expression; moreover, these associations were identified in both cell lines and a xenograft mouse model. Furthermore, we demonstrated that up-regulation of Cyclin D1 and the phosphorylated retinoblastoma protein (p-pRb) was mechanistically related to WT1 accelerating cells to S-phase. In conclusion, our findings demonstrated that WT1 is an oncogene and promotes NSCLC cell proliferation by up-regulating Cyclin D1 and p-pRb expression.
Structural elements of the ubiquitin-independent proteasome degron of ornithine decarboxylase (2008)

Mouse ODC (ornithine decarboxylase) is quickly degraded by the 26S proteasome in mammalian and fungal cells. Its degradation is independent of ubiquitin but requires a degradation signal composed of residues 425-461 at the ODC C-terminus, cODC (the last 37 amino acids of the ODC C-terminus). Mutational analysis of cODC revealed the presence of two essential elements in the degradation signal. The first consists of cysteine and alanine at residues 441 and 442 respectively. The second element is the C-terminus distal to residue 442; it has little or no sequence specificity, but is intolerant of insertions or deletions that alter its span. Reducing conditions, which preclude all well-characterized chemical reactions of the Cys(441) thiol, are essential for in vitro degradation. These experiments imply that the degradative function of Cys(441) does not involve its participation in chemical reaction; it, instead, functions within a structural element for recognition by the 26S proteasome.
Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8. (1989)

Abstract To identify possible regulatory sites of the gene expression, we cloned the genomic DNA of human monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8), whose mRNA is induced by IL-1 or TNF and determined its entire nucleotide sequence. The results show that the MDNCF/IL-8 gene consists of 4 exons and 3 introns with a single "CAT"- and "TATA"-like structure. The 5'-flanking region of MDNCF/IL-8 gene shows no overall sequence similarity with that of other cytokine and acute phase reactant genes whose production is also affected by IL-1 and TNF. The 5' flanking region, however, contains potential binding sites for several nuclear factors including activation factor-1, activation factor-2, IFN regulatory factor-1, and hepatocyte nuclear factor-1. In addition, the glucocorticoid responsive element and heat shock element were located in the 5'-flanking region. Inasmuch as PMA induces MDNCF/IL-8 mRNA accumulation in human PBMC and a glucocorticoid inhibits the induction of MDNCF/IL-8 mRNA by LPS, the expression of this gene is probably regulated by interaction of these nuclear factors with the 5'-flanking DNA.
Design Considerations for Robotic Needle Steering (2006)

Many medical procedures involve the use of needles, but targeting accuracy can be limited due to obstacles in the needle’s path, shifts in target position caused by tissue deformation, and undesired bending of the needle after insertion. In order to address these limitations, we have developed robotic systems that actively steer a needle in soft tissue. A bevel (asymmetric) tip causes the needle to bend during insertion, and steering is enhanced when the needle is very flexible. An experimental needle steering robot was designed that includes force/torque sensing, horizontal needle insertion, stereo image data acquisition, and controlled actuation of needle rotation and translation. Experiments were performed with a phantom tissue to determine the effects of insertion velocity and bevel tip angle on the needle path, as well as the forces acting on the needle during insertion. Results indicate that needle steering inside tissue does not depend on insertion velocity, but does depend on bevel tip angle. In addition, the forces acting on the needle are directly related to the insertion velocity.
LA FLEXIBILIDAD DE LA LOGÍSTICA COMO UNA ESTRATEGIA EMPRESARIAL EN LA INDUSTRIA DEL MUEBLE DE ESPAÑA (2013)

In a highly globalized market in which companies are currently developing, logistics appears as one business stategy applied in various organizations to achieve competitive advantage and stay in the market. Market pressure to reduce the costs of goods and increasingly customized products and services, are leading companies to adopt new business strategies, and logistics seems to be a good strategy to achieve their goals. Therefore, in this study with a sample of 322 companies in the furniture industry of Spain examines the impact that these companies flexibility in their logistics processes. The results show that physical supply flexibility, the flexibility of shopping, l to physical distribution flexibility and flexibility of demand management positively impact the flexibility of logistics companies, which can be considered as a good business strategy.
Adaptive motion vector resolution with implicit signaling (2010)

In all video coding standards, motion vectors are restricted to have the same resolution for the whole sequence. In this paper, we propose an adaptive motion vector resolution scheme to improve the coding efficiency. The scheme is based on an analytical model of motion compensated residue which studies the relationship between motion vector errors and the variance of the residue. With this model, the proposed scheme can adaptively select motion vector resolution with implicit signaling. Experimental results show that the proposed scheme can improve coding efficiency at slightly increased encoding complexity.
Sum-frequency spectroscopy of monolayers of alkoxy-terminated alkanethiols in contact with liquids (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSum-frequency spectroscopy of monolayers of alkoxy-terminated alkanethiols in contact with liquidsT. Hui Ong, Paul B. Davies, and Colin D. BainCite this: Langmuir 1993, 9, 7, 1836–1845Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/la00031a035https://doi.org/10.1021/la00031a035research-articleACS PublicationsRequest reuse permissionsArticle Views421Altmetric-Citations93LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Calorimetrically Determined Dynamics of Complex Unfolding Transitions in Proteins (1990)

Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific ...Read More
The American College of Rheumatology response criteria for systemic lupus erythematosus clinical trials: Measures of overall disease activity (2004)

To our knowledge, these are the first response criteria in any disease where a clinically relevant change has been determined a priori and mapped to standardized measures. This criterion should aid the clinical evaluation of new therapies, improve comparability between trials, and facilitate innovative trial designs.
Modeling High-Efficiency Quantum Dot Sensitized Solar Cells (2010)

With energy conversion efficiencies in continuous growth, quantum dot sensitized solar cells (QDSCs) are currently under an increasing interest, but there is an absence of a complete model for these devices. Here, we compile the latest developments in this kind of cells in order to attain high efficiency QDSCs, modeling the performance. CdSe QDs have been grown directly on a TiO(2) surface by successive ionic layer adsorption and reaction to ensure high QD loading. ZnS coating and previous growth of CdS were analyzed. Polysulfide electrolyte and Cu(2)S counterelectrodes were used to provide higher photocurrents and fill factors, FF. Incident photon-to-current efficiency peaks as high as 82%, under full 1 sun illumination, were obtained, which practically overcomes the photocurrent limitation commonly observed in QDSCs. High power conversion efficiency of up to 3.84% under full 1 sun illumination (V(oc) = 0.538 V, j(sc) = 13.9 mA/cm(2), FF = 0.51) and the characterization and modeling carried out indicate that recombination has to be overcome for further improvement of QDSC.
Light-Regulated Changes in Abundance and Polyribosome Association of Ferredoxin mRNA Are Dependent on Photosynthesis (1997)

Marie E. Petracek, Lynn F. Dickey, Steven C. Huber, William F. Thompson, Light-Regulated Changes in Abundance and Polyribosome Association of Ferredoxin mRNA Are Dependent on Photosynthesis, The Plant Cell, Vol. 9, No. 12 (Dec., 1997), pp. 2291-2300
Diabetes-Obesity Syndromes in Mice (1982)

Several different rodent models are available for metabolic studies on the development of diabetes. Although the abnormalities associated with each diabetes type have many features in common, the documentation of several different genes being involved makes it unlikely that the various syndromes will be reduced to a single disturbance in one metabolic pathway. The severity of the diabetes produced depends on the interaction of the individual mutation with genetic factors in the inbred background of the host. Establishing the nature of these gene-host interactions in rodents should aid us in understanding similar interactions that occur in human diabetes. The development of the syndrome in most models is similar and includes hyperinsulinemia, hyperphagia, and attempts at increasing insulin supply by beta-cell hyperplasia and hypertrophy in the early stages. Hyperglycemia, obesity, and severe diabetes are secondary features that result from a combination of insulin resistance and a failure to sustain the secretion of the large amounts of insulin. Most models utilize ingested food and stored food reserves more efficiently. This increased metabolic efficiency extends to heterozygotes that are normal in all respects having only one dose of the deleterious gene. Establishing this increased metabolic efficiency in heterozygotes lends credence to the thrifty gene hypothesis of diabetes and suggests a mechanism whereby some deleterious diabetes genes may be favored in the human population. The best studied mouse models, and those for which the most complete information is available, are those caused by single genes, e.g., yellow, obese, diabetes, tubby, and fat. In the other models, the mode of inheritance is either polygenic or otherwise unclear, features which interfere with the interpretation of the data. This report briefly summarizes the developing syndrome in each model, points out any differences, and suggests the most appropriate areas where future research should be most productive in the light of contemporary studies.
Reactivity of the essential thiol of Klebsiella aerogenes urease. Effect of pH and ligands on thiol modification (1991)

The kinetics of Klebsiella aerogenes urease inactivation by disulfide and alkylating agents was examined and found to follow pseudo-first-order kinetics. Reactivity of the essential thiol is affected by the presence of substrate and competitive inhibitors, consistent with a cysteine located proximal to the active site. In contrast to the results observed with other reagents, the rate of activity loss in the presence of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) saturated at high reagent concentrations, indicating that DTNB must first bind to urease before inactivation can occur. The pH dependence for the rate of urease inactivation by both disulfide and alkylating agents was consistent with an interaction between the thiol and a second ionizing group. The resulting macroscopic pKa values for the 2 residues are less than 5 and 12. Spectrophotometric studies at pH 7.75 demonstrated that 2,2'-dithiodipyridine (DTDP) modified 8.5 +/- 0.2 mol of thiol/mol of enzyme or 4.2 mol of thiol/mol of catalytic unit. With the slow tight binding competitive inhibitor phenyl-phosphorodiamidate (PPD) bound to urease, 1.1 +/- 0.1 mol of thiol/mol of catalytic unit were protected from modification. PPD-bound DTDP-modified urease could be reactivated by dialysis, consistent with the presence of one thiol per active site. Analogous studies at pH 6.1, using the competitive inhibitor phosphate, confirmed the presence of one protected thiol per catalytic unit. Under denaturing conditions, 25.5 +/- 0.3 mol of thiol/mol of enzyme (Mr = 211, 800) were modified by DTDP.
Glia as a Therapeutic Target: Selective Suppression of Human Amyloid-β-Induced Upregulation of Brain Proinflammatory Cytokine Production Attenuates Neurodegeneration (2006)

A corollary of the neuroinflammation hypothesis is that selective suppression of neurotoxic products produced by excessive glial activation will result in neuroprotection. We report here that daily oral administration to mice of the brain-penetrant compound 4,6-diphenyl-3-(4-(pyrimidin-2-yl)piperazin-1-yl)pyridazine (MW01-5-188WH), a selective inhibitor of proinflammatory cytokine production by activated glia, suppressed the human amyloid-beta (Abeta) 1-42-induced upregulation of interleukin-1beta, tumor necrosis factor-alpha, and S100B in the hippocampus. Suppression of neuroinflammation was accompanied by restoration of hippocampal synaptic dysfunction markers synaptophysin and postsynaptic density-95 back toward control levels. Consistent with the neuropathophysiological improvements, MW01-5-188WH therapy attenuated deficits in Y maze behavior, a hippocampal-linked task. Oral MW01-5-188WH therapy begun 3 weeks after initiation of intracerebroventricular infusion of human Abeta decreased the numbers of activated astrocytes and microglia and the cytokine levels in the hippocampus without modifying amyloid plaque burden or altering peripheral tissue cytokine upregulation in response to an in vivo inflammatory challenge. The results provide a novel integrative chemical biology proof in support of the neuroinflammation hypothesis of disease progression, demonstrate that neurodegeneration can be attenuated independently of plaque modulation by targeting innate brain proinflammatory cytokine responses, and indicate the feasibility of developing efficacious, safe, and selective therapies for neurodegenerative disorders by targeting key glial activation pathways.