gebhart/patrepeval huggingface dataset:
RosR (Cg1324), a Hydrogen Peroxide-sensitive MarR-type Transcriptional Regulator of Corynebacterium glutamicum* (2010)

The cg1324 gene (rosR) of Corynebacterium glutamicum encodes a MarR-type transcriptional regulator. By a comparative transcriptome analysis with DNA microarrays of a ΔrosR mutant and the wild type and subsequent EMSAs with purified RosR protein, direct target genes of RosR were identified. The narKGHJI operon, which encodes a nitrate/nitrite transporter and the dissimilatory nitrate reductase complex, was activated by RosR. All other target genes were repressed by RosR. They encode four putative monooxygenases, two putative FMN reductases, a protein of the glutathione S-transferase family, a putative polyisoprenoid-binding protein, and RosR itself. The DNA binding site of RosR was characterized as an 18-bp inverted repeat with the consensus sequence TTGTTGAYRYRTCAACWA. The in vitro DNA binding activity of RosR was reversibly inhibited by the oxidant H(2)O(2). Mutational analysis of the three cysteine residues present in RosR (Cys-64, Cys-92, and Cys-151) showed that these are responsible for the inhibition of DNA binding by H(2)O(2). A deletion mutant (Δcg1322) lacking the putative polyisoprenoid-binding protein showed an increased sensitivity to H(2)O(2), supporting the role of RosR in the oxidative stress response of C. glutamicum.
Design and experimental evaluation of multi-user beamforming in wireless LANs (2010)

Multi-User MIMO promises to increase the spectral efficiency of next generation wireless systems and is currently being incorporated in future industry standards. Although a significant amount of research has focused on theoretical capacity analysis, little is known about the performance of such systems in practice. In this paper, we present the design and implementation of the first multi-user beamforming system and experimental framework for wireless LANs. Using extensive measurements in an indoor environment, we evaluate the impact of receiver separation distance, outdated channel information due to mobility and environmental variation, and the potential for increasing spatial reuse. For the measured indoor environment, our results reveal that two receivers achieve close to maximum performance with a minimum separation distance of a quarter of a wavelength. We also show that the required channel information update rate is dependent on environmental variation and user mobility as well as a per-link SNR requirement. Assuming that a link can tolerate an SNR decrease of 3 dB, the required channel update rate is equal to 100 and 10 ms for non-mobile receivers and mobile receivers with a pedestrian speed of 3 mph respectively. Our results also show that spatial reuse can be increased by efficiently eliminating interference at any desired location; however, this may come at the expense of a significant drop in the quality of the served users.
Central circuitries for body temperature regulation and fever (2011)

Body temperature regulation is a fundamental homeostatic function that is governed by the central nervous system in homeothermic animals, including humans. The central thermoregulatory system also functions for host defense from invading pathogens by elevating body core temperature, a response known as fever. Thermoregulation and fever involve a variety of involuntary effector responses, and this review summarizes the current understandings of the central circuitry mechanisms that underlie nonshivering thermogenesis in brown adipose tissue, shivering thermogenesis in skeletal muscles, thermoregulatory cardiac regulation, heat-loss regulation through cutaneous vasomotion, and ACTH release. To defend thermal homeostasis from environmental thermal challenges, feedforward thermosensory information on environmental temperature sensed by skin thermoreceptors ascends through the spinal cord and lateral parabrachial nucleus to the preoptic area (POA). The POA also receives feedback signals from local thermosensitive neurons, as well as pyrogenic signals of prostaglandin E(2) produced in response to infection. These afferent signals are integrated and affect the activity of GABAergic inhibitory projection neurons descending from the POA to the dorsomedial hypothalamus (DMH) or to the rostral medullary raphe region (rMR). Attenuation of the descending inhibition by cooling or pyrogenic signals leads to disinhibition of thermogenic neurons in the DMH and sympathetic and somatic premotor neurons in the rMR, which then drive spinal motor output mechanisms to elicit thermogenesis, tachycardia, and cutaneous vasoconstriction. Warming signals enhance the descending inhibition from the POA to inhibit the motor outputs, resulting in cutaneous vasodilation and inhibited thermogenesis. This central thermoregulatory mechanism also functions for metabolic regulation and stress-induced hyperthermia.
Interaction Between Nitric Oxide Synthase and Cyclooxygenase Pathways in Osteoblastic MC3T3-E1 Cells (1997)

Interleukin 1 (IL-1) and tumor necrosis factor alpha (TNF-alpha) have been implicated in the pathogenesis of osteoporosis. These proinflammatory cytokines induce both cyclooxygenase (COX) and nitric oxide synthase (NOS) with the release of prostaglandin (PG) and NO, respectively. The present study was undertaken to examine the interaction between COX and NOS pathways and their role in the regulation of osteoblastic function in MC3T3-E1 cells. Addition of IL-1 alpha and TNF-alpha induced a marked increase in the production of both NO and PGE2. Reverse transcription-polymerase chain reaction analysis showed that the increase in NO production was preceded by the expression of inducible NOS mRNA. The temporal profile of PGE2 production revealed a biphasic pattern: the first small peak at 3 h was caused by de novo synthesis of PGE2 through inducible COX (COX-2) mRNA, while the subsequent progressive accumulation of PGE2 was mediated through the activation of COX pathway by NO since (1) aminoguanidine (AG), a selective inhibitor of inducible NOS, significantly suppressed the PGE2 production by IL-1 alpha and TNF-alpha, (2) NOC-18, an NO donor, reversed this suppression, and (3) NOC-18 increased PGE2 production by itself. The increase in NO production in response to IL-1 alpha and TNF-alpha was further stimulated by aspirin and inhibited by exogenous addition of PGE2, suggesting that PGE2 produced by the cytokines, in turn, negatively modulates NO production. IL-1 alpha and TNF-alpha inhibited alkaline phosphatase (ALP) activity, which was significantly reversed by AG. NOC-18 not only suppressed ALP activity by itself but also blocked the effect of AG, suggesting the role of NO in the inhibition of ALP activity. PGE2 decreased ALP activity, and the inhibitory effect of NOC-18 was attenuated in the presence of aspirin, suggesting the involvement of PGE2 in the negative modulation of ALP activity by NO. These results suggest that NO produced in response to proinflammatory cytokines participates in the modulation of ALP activity via the activation of COX pathway. The interaction between NO and the COX pathways may play an important role in the regulation of osteoblastic functions under physiologic as well as pathologic conditions.
The World Wide Web Unleashed (1994)

From the Publisher:
This is the only book to cover the Web-its uses, resources, and tools-in complete detail. Readers explore the farthest, most exotic reaches of the Web, and find out how to create and maintain Web servers for all types and uses.
Explores the Web's amazing world of electronic art museums, online magazines and catalogs, virtual malls, and video and music libraries
Shows how to navigate the World Wide Web, find Web resources and create Web pages
Includes a complete guide to using Mosaic and other Web tools
Synthesis of BILN 2061, an HCV NS3 Protease Inhibitor with Proven Antiviral Effect in Humans (2004)

The synthesis of BILN 2061, an NS3 protease inhibitor with proven antiviral effect in humans, was accomplished in a convergent manner from four building blocks. The procedure described here was suitable for the preparation of multigram quantities of BILN 2061 for preclinical pharmacological evaluation.
Definition of a new HLA‐B7 subtype (B*0704) by isoelectric focusing, family studies and DNA sequence analysis (1995)

During screening of potential bone marrow donors, a previously undescribed banding position for the serologically defined HLA-B7 antigen was identified in three unrelated families using one dimensional isoelectric focusing and class I specific Western blot analysis. The isoelectric point of the new variant is more acidic than the two HLA-B7 variants that had been defined before. In each family the new B7 variant was found linked to HLA-A2 and -Cw7. Cloning and sequencing of full-length clones of complementary DNA showed that the new allele (B*0704) differs from B*0702, the common allele encoding HLA-B7, by three nucleotide substitutions within the codon for residue 156 of the mature heavy chain. As a result of these differences amino acid 156 is changed from arginine to aspartic acid, a difference consistent with the isoelectric points. The group of three nucleotide substitutions that distinguish B*0704 from B*0702 is present in other HLA-B alleles.
Type IV Hypersensitivity Reaction (2021)

Our immune system plays a crucial role in protecting our body against pathogens, but sometimes there is an exaggerated response. This exaggerated response is triggered by the interaction of the immune system with an antigen (allergen) and is referred to as hypersensitivity. Hypersensitivity reactions are classified into four types by Coombs and Gell. The first three types are considered immediate hypersensitivity reactions because they occur within 24 hours. The fourth type is considered a delayed hypersensitivity reaction because it usually occurs more than 12 hours after exposure to the allergen, with a maximal reaction time between 48 and 72 hours. The four types of hypersensitivity are: Type I: reaction mediated by IgE antibodies Type II: cytotoxic reaction mediated by IgG or IgM antibodies Type III: reaction mediated by immune complexes Type IV: delayed reaction mediated by cellular response A Type IV hypersensitivity reaction is mediated by T cells that provoke an inflammatory reaction against exogenous or endogenous antigens. In certain situations, other cells, such as monocytes, eosinophils, and neutrophils, can be involved. After antigen exposure, an initial local immune and inflammatory response occurs that attracts leukocytes. The antigen engulfed by the macrophages and monocytes is presented to T cells, which then becomes sensitized and activated. These cells then release cytokines and chemokines, which can cause tissue damage and may result in illnesses. Examples of illnesses resulting from type IV hypersensitivity reactions include contact dermatitis and drug hypersensitivity. Type IV reactions are further subdivided into type IVa, IVb, IVc, and IVd based on the type of T cell (CD4 T-helper type 1 and type 2 cells) involved and the cytokines/chemokines produced. Delayed hypersensitivity plays a crucial role in our body's ability to fight various intracellular pathogens such as mycobacteria and fungi. They also play a principal role in tumor immunity and transplant rejection. Since patients with acquired immunodeficiency syndrome (AIDS) have a progressive decline in the number of CD4 cells, they also have a defective type four hypersensitivity reaction.
沙特阿拉伯海军“阿尔·里雅德”号新型隐形多用途护卫舰 (2001)

“阿尔·里雅德”号隐形护卫舰(AL RIYADH舷号812)是法国海军拉斐特级隐形护卫舰的改进型,由法国海军舰船制造局洛里昂造船厂建造,型号名称F-3000S,2001年9月17日首次海上试航。该舰满载排水量4650吨(比原型舰多950吨),全长135米,两座燃气轮机驱动,主机20.6兆瓦,最大航速25节。该照片为舰艏部特写。
The therapeutic potential of targeting the cell cycle (1998)

With the advent of modern molecular genetics, molecular biology and biochemistry has come a revolution in oncology drug discovery research. We are rapidly developing an increased understanding in the mechanisms driving cellular proliferation, transformation, differentiation and metastasis. The hope is that from these advances will emerge novel therapeutics that are more specific, more efficacious and less toxic than their predecessors. Uncontrolled proliferation is a hallmark of a cancer cell. Over the past two decades it has become increasingly clear that molecules that directly control cell cycle progression accumulate defects during tumourigenesis. These defects can result in the loss of checkpoint control and/or the inappropriate activation of the 'drivers' of cell cycle progression, the cyclin-dependent kinases (cdks). This review will describe the recent advances in our understanding of cell cycle regulation and its relation to tumourigenesis, and highlight the potential for the development of novel anticancer therapeutics.
Cuffless Blood Pressure Monitoring Using Hydrostatic Pressure Changes (2008)

This paper presents a new principle for noninvasive blood pressure measurements through a modified volume-oscillometric technique that eliminates an inflatable pressure cuff, and instead takes advantage of natural hydrostatic pressure changes caused by raising and lowering the subject's arm. This new methodology provides the distinct advantage of using an absolute gauge pressure reference for measurements, and does not necessarily require additional actuation.
Knee Simulation, Creep, and Friction Tests of Poly(vinyl alcohol) Hydrogels Manufactured Using Injection Molding and Solution Casting (2022)

Osteoarthritis is a widespread problem throughout the world especially as the average lifespan continues to lengthen. Relieving arthritic pain and finding less intrusive, longer lasting joint replacements revolves around finding a material to replace natural articular cartilage. Poly(vinyl alcohol) hydrogels (PVA-H) have the potential to be an articular cartilage replacement in the future with properties similar to those of natural articular cartilage. Injection molding and solution casting techniques were employed to manufacture PVA-H specimens. The mechanical properties of PVA-H were then studied using knee simulation, creep, and friction tests. The results show that injection molding produces stiffer and stronger specimens than solution casting due to the higher polymer content. However, injection molding specimens also have a higher coefficient of friction. Further improvements must be made before PVA-H is a suitable material for articular cartilage replacement.
Alba: ¿un proyecto alternativo para América Latina? (2008)

La propuesta venezolana/cubana del ALBA (Alternativa Bolivariana para las Américas), a la que se suman Bolivia, Nicaragua y Dominica, aparece como un proyecto alternativo de integración en América Latina y el Caribe. Se analiza el origen, desarrollo y propuestas de esta iniciativa. La VI Cumbre del ALBA (Alternativa Bolivariana para las Américas) realizada el 26 de enero de 2008 en Caracas, generó gran desconcierto con el anuncio de crear una alianza militar de los cinco Estados miembros del ALBA; propuesta de integración que se autodefine como “un esfuerzo de construcción del proyecto global latinoamericano, con una correlación de fuerzas políticas favorables en el continente que le permiten consolidarse como alternativa política y económica”. ¿Es este enunciado real y en verdad es el ALBA un proyecto de integración alternativo con viabilidad política, económica y estratégica para Latinoamérica, o simplemente es una contrapropuesta política al Área de Libre Comercio para las Américas (ALCA) impulsada desde Washington? El discurso del ALBA aparece fortalecido en la región, pero su propuesta de integración –incluido su proyecto militar– es inviable. No obstante, sus acciones efectivas a través de mecanismos como Petrocaribe, muestran una viabilidad e incidencia mucho mayor que la que los principales actores internacionales le endosan.
Circumvention of Methotrexate Resistance in Childhood Leukemia Subtypes by Rationally Designed Antifolates (1999)

Cellular methotrexate (MTX) resistance may cause treatment failure in childhood common/preB-acute lymphoblastic leukemia (c/preB-ALL), T-lineage ALL (T-ALL), and acute myeloid leukemia (AML). The ex vivo potency of several antifolates (MTX, trimetrexate [TMQ], GW1843U89, multitargeted antifolate [MTA], Raltitrexed, and ZD9331) was studied via in situ inhibition of thymidylate synthase (TS). After short-term exposure, relapsed c/preB-ALL (rALL, n = 21), T-ALL (n = 22), and AML (n = 22) were 3-fold, 10-fold, and 6-fold less sensitive to MTX (P ≤ .01) compared with initial c/preB-ALL (n = 43). This difference in resistance was not observed for TMQ. Also for GW1843U89 and MTA, no resistance was observed in rALL and AML compared with c/preB-ALL. T-ALL compared with c/preB-ALL tended to be less resistant to GW1843U89 (3-fold) and MTA (6-fold) than to MTX (10-fold) (P= .06). Raltitrexed was more active against c/preB-ALL compared with the other subtypes. After 21 hours continuous incubation, T-ALL and AML samples were equally sensitive as c/preB-ALL to MTX, but rALL was 3-fold resistant to MTX compared with initial c/preB-ALL (P = .003). The resistance of rALL was circumvented by TMQ (1-fold; P = .03) and GW1843U89 (1.4-fold; P= .004). Novel antifolates, except MTA, displayed a more potent TS inhibition than MTX during continuous exposure. These results suggest that MTX resistance in AML and T-ALL can be circumvented by continuous exposure, and that novel antifolates should be explored further for MTX-resistant T-ALL, rALL, and AML cells.
Messenger RNA Electroporation of Human Monocytes, Followed by Rapid In Vitro Differentiation, Leads to Highly Stimulatory Antigen-Loaded Mature Dendritic Cells (2002)

Dendritic cells (DC) are professional Ag-capturing and -presenting cells of the immune system. Because of their exceptional capability of activating tumor-specific T cells, cancer vaccination research is now shifting toward the formulation of a clinical human DC vaccine. We developed a short term and serum-free culture protocol for rapid generation of fully mature, viable, and highly stimulatory CD83(+) DC. Human monocytes were cultured for 24 h in serum-free AIM-V medium, followed by 24-h maturation by polyriboinosinic polyribocytidylic acid (polyI:C). Short term cultured, polyI:C-maturated DC, far more than immature DC, showed typical mature DC markers and high allogeneic stimulatory capacity and had high autologous stimulatory capacity in an influenza model system using peptide-pulsed DC. Electroporation of mRNA as an Ag-loading strategy in these cells was optimized using mRNA encoding the enhanced green fluorescent protein (EGFP). Monocytes electroporated with EGFP mRNA, followed by short term, serum-free differentiation to mature DC, had a phenotype of DC, and all showed positive EGFP fluorescence. Influenza matrix protein mRNA-electroporated monocytes cultured serum-free and maturated with polyI:C showed high stimulatory capacity in autologous T cell activation experiments. In conclusion, the present short term and serum-free ex vivo DC culture protocol in combination with mRNA electroporation at the monocyte stage imply an important reduction in time and consumables for preparation of Ag-loaded mature DC compared with classical DC culture protocols and might find application in clinical immunotherapy settings.
Normobaric Hyperoxia Reduces the Neurovascular Complications Associated With Delayed Tissue Plasminogen Activator Treatment in a Rat Model of Focal Cerebral Ischemia (2009)

Our results suggest that early normobaric hyperoxia treatment may represent an important strategy to increase the safety of delayed tPA thrombolysis in ischemic stroke.
Modeling the performance of a photon counting x‐ray detector for CT: Energy response and pulse pileup effects (2011)

It has been demonstrated that the performance of the DXMCT-1 agreed exceptionally well with the analytical models regarding the energy response, the count rate, and the recorded spectrum with pulse pileup effects. These models will be useful in developing methods to compensate for these effects in PCXD-based clinical CT systems.
Process variation aware thread mapping for Chip Multiprocessors (2009)

With the increasing scaling of manufacturing technology, process variation is a phenomenon that has become more prevalent. As a result, in the context of Chip Multiprocessors (CMPs) for example, it is possible that identically-designed processor cores on the chip have non-identical peak frequencies and power consumptions. To cope with such a design, each processor can be assumed to run at the frequency of the slowest processor, resulting in wasted computational capability. This paper considers an alternate approach and proposes an algorithm that intelligently maps (and remaps) computations onto available processors so that each processor runs at its peak frequency. In other words, by dynamically changing the thread-to-processor mapping at runtime, our approach allows each processor to maximize its performance, rather than simply using chip-wide lowest frequency amongst all cores and highest cache latency. Experimental evidence shows that, as compared to a process variation agnostic thread mapping strategy, our proposed scheme achieves as much as 29% improvement in overall execution latency, average improvement being 13% over the benchmarks tested. We also demonstrate in this paper that our savings are consistent across different processor counts, latency maps, and latency distributions.With the increasing scaling of manufacturing technology, process variation is a phenomenon that has become more prevalent. As a result, in the context of Chip Multiprocessors (CMPs) for example, it is possible that identically-designed processor cores on the chip have non-identical peak frequencies and power consumptions. To cope with such a design, each processor can be assumed to run at the frequency of the slowest processor, resulting in wasted computational capability. This paper considers an alternate approach and proposes an algorithm that intelligently maps (and remaps) computations onto available processors so that each processor runs at its peak frequency. In other words, by dynamically changing the thread-to-processor mapping at runtime, our approach allows each processor to maximize its performance, rather than simply using chip-wide lowest frequency amongst all cores and highest cache latency. Experimental evidence shows that, as compared to a process variation agnostic thread mapping strategy, our proposed scheme achieves as much as 29% improvement in overall execution latency, average improvement being 13% over the benchmarks tested. We also demonstrate in this paper that our savings are consistent across different processor counts, latency maps, and latency distributions.
Regional Myocardial Metabolism of High-Energy Phosphates during Isometric Exercise in Patients with Coronary Artery Disease (1990)

The decrease in the ratio of phosphocreatine to ATP during hand-grip exercise in patients with myocardial ischemia reflects a transient imbalance between oxygen supply and demand in myocardium with compromised blood flow. Exercise testing with 31P NMR is a useful method of assessing the effect of ischemia on myocardial metabolism of high-energy phosphates and of monitoring the response to treatment.
Ultrasmall Gold Nanorod Vesicles with Enhanced Tumor Accumulation and Fast Excretion from the Body for Cancer Therapy (2015)

A new kind of ultrasmall dissociable AuNR@PEG/PLGA vesicles (≈60 nm) (AuNR = gold nanorod; PEG = poly(ethylene glycol); PLGA = poly(lactic-co-glycolic acid)) assembled from small AuNRs (dimension: ≈8 nm × 2 nm) is reported. They exhibit several striking features: prolonged circulation and prominent tumor accumulation; rapid excretion from the body as AuNR@PEG after therapy; enhanced photoacoustic and photo thermal properties; and high photothermal cancer therapy efficacy.