gebhart/patrepeval huggingface dataset:
HermesD: A High-Rate Long-Range Wireless Transmission System for Simultaneous Multichannel Neural Recording Applications (2010)

HermesD is a high-rate, low-power wireless transmission system to aid research in neural prosthetic systems for motor disabilities and basic motor neuroscience. It is the third generation of our "Hermes systems" aimed at recording and transmitting neural activity from brain-implanted electrode arrays. This system supports the simultaneous transmission of 32 channels of broadband data sampled at 30 ks/s, 12 b/sample, using frequency-shift keying modulation on a carrier frequency adjustable from 3.7 to 4.1 GHz, with a link range extending over 20 m. The channel rate is 24 Mb/s and the bit stream includes synchronization and error detection mechanisms. The power consumption, approximately 142 mW, is low enough to allow the system to operate continuously for 33 h, using two 3.6-V/1200-mAh Li-SOCl2 batteries. The transmitter was designed using off-the-shelf components and is assembled in a stack of three 28 mm ? 28-mm boards that fit in a 38 mm ? 38 mm ? 51-mm aluminum enclosure, a significant size reduction over the initial version of HermesD. A 7-dBi circularly polarized patch antenna is used as the transmitter antenna, while on the receiver side, a 13-dBi circular horn antenna is employed. The advantages of using circularly polarized waves are analyzed and confirmed by indoor measurements. The receiver is a stand-alone device composed of several submodules and is interfaced to a computer for data acquisition and processing. It is based on the superheterodyne architecture and includes automatic frequency control that keeps it optimally tuned to the transmitter frequency. The HermesD communications performance is shown through bit-error rate measurements and eye-diagram plots. The sensitivity of the receiver is -83 dBm for a bit-error probability of 10(-9). Experimental recordings from a rhesus monkey conducting multiple tasks show a signal quality comparable to commercial acquisition systems, both in the low-frequency (local field potentials) and upper-frequency bands (action potentials) of the neural signals. This system can be easily scaled up in terms of the number of channels and data rate to accommodate future generations of Hermes systems.
The Effect of Insulin Growth Factor-1 on Calvarial Sutures in a Sprague-Dawley Rat (1993)

Early investigations attempting to determine the exact pathogenesis of premature closure of the cranial sutures were limited by the untoward sequelae caused by surgical procedures in animal models to simulate the development of craniosynostosis. In an attempt to create a model for evaluating the effects of manipulating the craniofacial sutures without the limitations of associated scarring, we conceived an animal prototype dependent on the effects of insulin growth factor-1 on the anterior frontal suture in a Sprague-Dawley rat model. The experimental group received 2 mg of IGF-1 over a 14-day period via a subcutaneously placed osmotic infusion pump, whereas the age-matched control group received no treatment. We present our preliminary histological results describing the effect of IGF-1 on calvarial sutures.
Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay (2000)

A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.
Study of Insertion of Olefins and/or Carbon Monoxide into Phosphine−Imine Palladium Methyl Complexes (2001)

Palladium methyl complexes with a phosphine−imine (P∼N) bidentate ligand, [Pd(P∼N)MeCl] (1), [Pd(P∼N)Me(CH3CN)](BF4) (2), and [Pd(P∼N)Me(PPh3)](BF4) (3), are treated with CO to result in the corresponding Pd−acyl complexes 4−6. NMR studies indicate the formation of a sole isomer with the acyl group cis to the phosphine donor. The neutral complex 4 and the triphenylphosphine-substituted complex 6 cannot serve for the insertion of ethylene. However, the acetonitrile complex 5 appears to be active in the insertion reaction with various alkenes as well as ethylene/CO (E-CO) co-oligomerization, resulting in the products 7−14 and 16, which have been isolated and characterized by spectroscopic methods. X-ray structures of 1, 2, 3, 4, 7, 10, 11, and 13 are revealed.
Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers (1989)

An efficient method for the construction of multiple mutations in a sequential manner is described. It is based on the gapped duplex DNA approach to oligonucleotide-directed mutagenesis (Kramer et al. 1984, Nucl. Acids Res. 12, 9441-9456) and a set of newly constructed phasmid vectors. These are characterized by the following features. Presence of the phage fl replication origin permits ready conversion to the single stranded DNA form. An amber mutation within, alternatively, the bla or cat gene provides a means for ready selection of the strand into which the mutagenic oligonucleotide has been incorporated. By means of the alternating antibiotic resistance markers any number of mutations can be constructed in consecutive rounds of mutagenesis. The optional presence of gene expression signals allows the direct overproduction of structurally altered proteins without re-cloning. Both the mutagenesis and expression aspects were tested using the lacZ gene as a model.
Discrete radon transform (1987)

This paper describes the discrete Radon transform (DRT) and the exact inversion algorithm for it. Similar to the discrete Fourier transform (DFT), the DRT is defined for periodic vector-sequences and studied as a transform in its own right. Casting the forward transform as a matrix-vector multiplication, the key observation is that the matrix-although very large-has a block-circulant structure. This observation allows construction of fast direct and inverse transforms. Moreover, we show that the DRT can be used to compute various generalizations of the classical Radon transform (RT) and, in particular, the generalization where straight lines are replaced by curves and weight functions are introduced into the integrals along these curves. In fact, we describe not a single transform, but a class of transforms, representatives of which correspond in one way or another to discrete versions of the RT and its generalizations. An interesting observation is that the exact inversion algorithm cannot be obtained directly from Radon's inversion formula. Given the fact that the RT has no nontrivial one-dimensional analog, exact invertibility makes the DRT a useful tool geared specifically for multidimensional digital signal processing. Exact invertibility of the DRT, flexibility in its definition, and fast computational algorithm affect present applications and open possibilities for new ones. Some of these applications are discussed in the paper.
Triple-negative breast cancer is associated with EGFR, CK5/6 and c-KIT expression in Malaysian women (2012)

In conclusion, our study confirms the association of TNBC with basal-like marker expression (EGFR, CK5/6 and c-KIT) in Malaysian women, consistent with studies in other populations.
All-phase motion compensated prediction in the wavelet domain for high performance video coding (2002)

This paper presents a novel framework of motion compensated prediction (MCP) techniques in the wavelet domain for high performance video coding. Our analysis reveals fundamental limitations with previous ad-hoc wavelet-based video coders from the motion accuracy point of view. We demonstrate that the phase associated with any wavelet transform carries critical information of the motion accuracy and we propose to restore the motion accuracy by considering the wavelet coefficients of the previous frame with all different phases. Our all-phase MCP approach can be viewed as predicting the wavelet coefficients from an over-complete expansion of the previous frame. Experimental results have shown that restoration of motion accuracy in the wavelet domain can dramatically improve the efficiency of MCP. The video coder (MCP-WT) built upon the MCP of wavelet coefficients has achieved 2 to 3 dB gain over existing the MPEG-2 coder at the bit rate of 1 to 9 Mbps. Moreover, MCP techniques in the wavelet domain offer a promising new ground for developing efficient scalable video coders.
Maladie de Fabry (déficit en α-galactosidase A) : innovations thérapeutiques récentes (2002)

Fabry disease (FD, OMIM 301500) is an X-linked inherited disorder of metabolism due to mutations in the gene encoding alpha-galactosidase A, a lysosomal enzyme. The enzymatic defect leads to the accumulation of neutral glycosphingolipids throughout the body, particularly within endothelial cells. Resulting narrowing and tortuosity of small blood vessels with endothelial dysfunction lead to tissue ischaemia and infarction. Inability to prevent the progression of glycosphingolipid deposition causes significant morbidity and mortality from early onset strokes, cardiomyopathy and renal failure in adulthood. Medical management is symptomatic and consists of partial pain relief with analgesic drugs (gabapentin, carbamazepine), antihypertensive drugs, whereas renal transplantation or dialysis is available for patients experiencing end-stage renal failure. However, the ability to produce high doses of alpha-galactosidase A in vitro has opened the way to preclinical studies in the mouse model, and to the development of the first clinical trials in patients with Fabry disease. Enzyme replacement therapy has recently been validated as a therapeutic agent for Fabry disease patients. Long term safety and efficacy of replacement therapy are currently being investigated. Substrate deprivation and gene therapy may also prove future alternative therapeutic options.
CATALYSIS OF HETEROPOLY ACIDS ENTRAPPED IN ACTIVATED CARBON (1981)

Abstract Activated carbon was found to be able to entrap a certain amount of heteropoly acids, resulting in solid acid catalysts. Heteropoly acids thus entrapped were hardly removed even by extraction with hot water or hot methanol. The entrapped catalysts offer convenient methods for liquid-phase etherification of alcohols and vapor-phase selective esterification.
The glutamate antagonist MK‐801 reduces focal ischemic brain damage in the rat (1988)

Excessive activation of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor has been implicated in the sequence of neurochemical events that results in irreversible neuronal damage in cerebral ischemia. The effects of the NMDA antagonist (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK-801) upon the amount of ischemic brain damage has been assessed quantitatively in the lightly anesthetized rat. Focal cerebral ischemia was produced by the permanent occlusion of one middle cerebral artery (MCA), and the animals were killed 3 hours after the arterial occlusion. MK-801 (0.5 mg/kg) was administered intravenously either 30 minutes prior to MCA occlusion or 30 minutes after the induction of ischemia. Pretreatment with MK-801 reduced the volume of ischemic damage both in the cerebral cortex (by 38% compared with untreated rats with MCA occlusion; p less than 0.01) and in the caudate nucleus (by 18% compared with controls; p less than 0.05). Treatment with MK-801, initiated 30 minutes after MCA occlusion, reduced the volume of ischemic damage in the cerebral cortex (by 52% compared with controls; p less than 0.01). The volume of ischemic damage in the caudate nucleus was minimally influenced by MK-801 treatment initiated after MCA occlusion. The antiischemic effects of MK-801 were readily demonstrable despite the hypotension that MK-801 induced in rats anesthetized with halothane (0.5%), nitrous oxide (70%), and oxygen (30%). The potency of MK-801 in reducing ischemic brain damage, even when administered after the induction of ischemia, highlights the potential use of NMDA receptor antagonists for the treatment of focal cerebral ischemia in humans.
Non-antibiotic Resistance Selection Marker Systems for Lactic Acid Bacteria (2007)

Lactic acid bacteria (LAB) are safe bacteria with a GRAS (generally regarded as safe) status. They have long been recognized as essential constituents of the microflora present in food products or live vehicles for expression of heterologous proteins. The considerable exploit of lactic acid bacteria and the development of molecular biology have allowed to construct many non-antibiotic resistance selection markers for LAB that can be categorized into four kinds: dominant selection marker, complementation selection marker, dominant/complementation selection marker and double-plasmid selection marker. The principle,application, advantages, defection and the recently development of these non-antibiotic resistance selection markers are reviewed in this paper. These selection marker systems are potential for variety of application in food fermentations or live vehicles and so on.
Chemistry of Protein Conjugation and Cross Linking (1991)

INTRODUCTION REACTIVE GROUPS OF PROTEINS AND THEIR MODIFYING AGENTS Compositions of Proteins Functional Groups of Proteins Group Directed Reagents CHOICE AND DESIGN OF CROSS-LINKING REAGENTS Nucleophilic Reactions Electrophilic Reactions Group Directed Reactions Nonspecific Reactions Cleavable Reagents Labeled Cross-Linking Reagents Hydrophobicity and Hydrophilicity of Reagents HOMOBIFUNCTIONAL CROSS-LINKING REAGENTS Amino Group Directed Cross-Linkers Sulfhydryl Group Directed Cross-Linkers Carboxyl Group Directed Cross-Linking Reagents Phenolate and Imidazolyl Group Directed Cross-Linking Reagents Arginine Residue Directed Cross-Linker Nondiscriminatory Photoactivatable Cross-Linkers Noncovalent Homobifunctional Cross-Linking Reagents Miscellaneous Bifunctional Reagents HETEROBIFUNCTIONAL CROSS-LINKERS Group Selective Heterobifunctional Cross-Linking Reagents Photosensitive Heterobifunctional Cross-Linking Reagents Disguised or Masked Reagents ZERO-LENGTH CROSS-LINKING REAGENTS Carboxyl Group Activating Reagents Reagents for Carbohydrate Activation Reagents for Disulfide Formation Enzymes as Zero-Length Cross-Linkers PROCEDURES, ANALYSIS, AND COMPLICATIONS Procedures for Cross-Linking Analysis of Cross-Linked Products Reaction Complications APPLICATION OF CHEMICAL CROSS-LINKING TO SOLUBLE PROTEINS Interresidue Distance Determinations Nearest Neighbor Analysis Conformational Analysis Structural Stability CROSS-LINKING OF MEMBRANE PROTEINS General Methodology of Membrane Cross-Linking Possible Artifacts of Membrane Cross-Linking Identification of Membrane Receptors Nearest-Neighbor Analysis of Membrane Components Membrane Protein Conformational Analysis Stabilization of Membrane Proteins PREPARATION OF ENZYME IMMUNOCONJUGATES AND OTHER IMMUNOASSAY COMPONENTS Components of Enzyme Immunoassays Conjugation of Enzymes to Antibodies and their Fragments Coupling Proteins to Antibodies and their Fragments Coupling Enzymes to Proteins and Antigens Characterization of Conjugation Methods PREPARATION OF IMMUNOTOXINS AND OTHER DRUG CONJUGATES FOR TARGETING THERAPEUTICS Selection of Cross-Linking Reagents and Toxins Conjugation through Disulfide Bond Conjugation through Thioether Linkage Conjugation with Actuvated Chlorambucil Conjugation with Acid-Labile and Photocleaveable Cross-Linkers Conjugation through Carbohydrate Residues Preparation of Immunotoxins Using Avidin-Biotin Linkage Miscellaneous Coupling Methods CONJUGATION OF PROTEINS TO SOLID MATRICES Functionalities of Matrices Protein Immobilization by Matrix Activation Cross-Linking Reagents Used in Protein Immobilization Cross-Linking through Carbohydrate Chains OTHER APPLICATIONS AND FUTURE PROSPECTS Application to Nucleic Acids Cross-Linking Small Molecules to Proteins Synopsis INDEX Note: Each chapter also contains an Introduction and References
Cell Lines for the Production of Recombinant Adeno-Associated Virus (1995)

Adeno-associated virus (AAV) is a replication-defective parvovirus that is being developed as a vector for human gene transfer. However, a major obstacle to commonplace usage of AAV vectors is the production of recombinant virions (rAAV) in sufficient quantities for not only human trials, but also for preclinical studies of basic biology, toxicology, and efficacy. Unfortunately, current methods for large-scale production are cumbersome and expensive. We have developed a simplified method for generating rAAV by establishing neomycin-resistant cell lines containing copies of the AAV rep-cap genes and a rAAV vector. After infection with adenovirus, these cell lines are shown to produce infectious rAAV in relatively high titer. This method eliminates the need for exogenous DNA transfection and scale-up procedures are limited only by the normal constraints of growing cells in culture.
Marijuana use and epilepsy (2004)

The authors sought to determine the prevalence of marijuana use in patients with epilepsy by performing a telephone survey in a tertiary care epilepsy center. Twenty-one percent of subjects had used marijuana in the past year with the majority of active users reporting beneficial effects on seizures. Twenty-four percent of all subjects believed marijuana was an effective therapy for epilepsy. Despite limited evidence of efficacy, many patients with epilepsy believe marijuana is an effective therapy for epilepsy and are actively using it.
Ejection Time by Ear Densitogram and Its Derivative (1973)

Ear densitographic ejection times (EDET) and first derivative ear densitogram ejection times (dEDET) were studied to determine whether their reliability and validity justify their substitution for ejection times derived from the far less stable carotid pulse tracing. Inter- and intra-subject comparisons were made on thirty individuals under a wide variety of disease and challenge states. Statistical analysis of the data-which had been obtained through a blinded procedure-showed an overall correlation ( r ) of .98 for carotid vs EDET and .99 for carotid vs dEDET. The t -test demonstrated no significant differences among ejection times derived from the three methods. Moreover, the close tracking at rest and during challenges of ejection times derived from these curves with those from the carotid indicate that either method may be substituted for standard carotid curves without sacrificing reliability or validity of the measure. Both ear curves offer distinct advantages over carotid pulse curves because their sensor is self-retaining and they remain stable during exercise and other body and respiratory movements. The additional feature of simplicity in reading the first derivative of the ear densitogram over its undifferentiated curve makes dEDET the preferred method.
Regulation of Immune Response by P2X7 Receptor (2006)

The P2X7 receptor is an ATP-gated cation channel that is widely expressed in cells of the immune system. Signal transduction is accompanied by fast influx of Ca2+ and Na+, and efflux of K+. This receptor differs from other members of the P2X family in its relatively low affinity for ATP, the presence of a long C-terminal region that contains several protein-protein interaction motifs, and the activation of two membrane conductance states following receptor ligation. In the immune system, this receptor has been implicated in the processing and release of cytokines such as IL-1 beta, and in the initiation of cell death via both apoptotic and necrotic pathways. As such, it has been proposed to function as a major regulator of inflammation. Consistent with this hypothesis, inactivation of this receptor in mice modulates disease pathogenesis in several animal models of inflammatory and autoimmune diseases. Loss-of-function polymorphisms have also been noted in the human population, and there is accumulating evidence that these polymorphisms are linked to certain diseases. In this article, we review the current status of research in this field, with particular emphasis on the signaling pathways activated by this receptor, the mechanisms involved in the initiation of cell death, and associations with disease states in mice and humans.
[The eye and pregnancy]. (1991)

The principal effects of pregnancy on the eye are reviewed, these effects being subdivided into three main sections: paraphysiological changes, pathological conditions developing in pregnancy and related to it, pre-existing pathological conditions that are to some extent altered by pregnancy. On the basis of the data supplied, a continuous, increasingly assiduous clinico-experimental give-and-take between oculist and gynaecologist is recommended to prevent possible eye complications.
Monoclonal antibody αIR-3 inhibits non-small cell lung cancer growth in vitro and in vivo (1996)

The ability of monoclonal antibody (mAb) alpha IR-3 to interact with non-small cell lung cancer (NSCLC) cells was investigated. MAb alpha IR-3 inhibited specific binding of 125I-IGF-I and 125I-alpha IR-3 to a panel of 8 NSCLC cell lines with high affinity (IC50 = 200 and 50 ng/ml, respectively). 125I-alpha IR-3 bound with high affinity (Kd = 40 ng/ml) to a single class of sites (Bmax = 8,000/cell) using NCI-H838 cells. 125I-alpha IR-3 was internalized when exposed to NCI-H838 or H1299 cells at 37 degrees C but not 4 degrees C. alpha IR-3 immunoprecipitated major 90 and 130 kD proteins. IGF-I stimulated and alpha IR-3 inhibited the clonal growth of NCI-H1299 cells. alpha IR-3 slowed the growth of NCI-H157 and H838 xenografts in nude mice. In a biodistribution study 125I-alpha IR-3 was preferentially localized to the tumor as opposed to other organs. These data suggest that IGF-I may be a regulatory agent in NSCLC.