gebhart/patrepeval huggingface dataset:
Thyroid hormone and breast carcinoma (2005)

Primary HYPT was associated with a reduced risk for PBC and a more indolent invasive disease. These data suggest a possible biologic role for thyroid hormone in the etiology of breast carcinoma and indicate areas of research for the prevention and treatment of breast carcinoma.
The dual‐mode solution and transport of water in poly(acrylonitrile) (1983)

Abstract Solubility (sorption) and transport measurements with water in solvent‐free poly (acrylonitrile)(PAN) (23) have been interpreted in terms of an extension of the “dual‐sorption” model for transport of small molecules in glassy polymers. Satisfactory agreement has been obtained between theory and experimental data. An earlier analysis of similar data for water in PAN containing 0.26 percent (by weight) of a residual solvent (1) has been compared with the present results in order to investigate the effects of residual solvent on the dual‐sorption and transport parameters for the water‐PAN system.
Cell separation using tilted-angle standing surface acoustic waves (2014)

Separation of cells is a critical process for studying cell properties, disease diagnostics, and therapeutics. Cell sorting by acoustic waves offers a means to separate cells on the basis of their size and physical properties in a label-free, contactless, and biocompatible manner. The separation sensitivity and efficiency of currently available acoustic-based approaches, however, are limited, thereby restricting their widespread application in research and health diagnostics. In this work, we introduce a unique configuration of tilted-angle standing surface acoustic waves (taSSAW), which are oriented at an optimally designed inclination to the flow direction in the microfluidic channel. We demonstrate that this design significantly improves the efficiency and sensitivity of acoustic separation techniques. To optimize our device design, we carried out systematic simulations of cell trajectories, matching closely with experimental results. Using numerically optimized design of taSSAW, we successfully separated 2- and 10-µm-diameter polystyrene beads with a separation efficiency of ∼ 99%, and separated 7.3- and 9.9-µm-polystyrene beads with an efficiency of ∼ 97%. We illustrate that taSSAW is capable of effectively separating particles-cells of approximately the same size and density but different compressibility. Finally, we demonstrate the effectiveness of the present technique for biological-biomedical applications by sorting MCF-7 human breast cancer cells from nonmalignant leukocytes, while preserving the integrity of the separated cells. The method introduced here thus offers a unique route for separating circulating tumor cells, and for label-free cell separation with potential applications in biological research, disease diagnostics, and clinical practice.
Clinical trials comparing norepinephrine with vasopressin in patients with septic shock: a meta-analysis (2014)

There is not sufficient evidence to prove conclusively that norepinephrine is superior to vasopressin in terms of mortality and hemodynamics. The effects of norepinephrine and vasopressin on patients with septic shock require further study in large randomized controlled trials.
Clinical aspects of MuSK antibody positive seronegative MG (2003)

Serum antibodies to muscle-specific receptor tyrosine kinase were detected in 12 of 32 patients with generalized seronegative MG. All were women, with onset between ages 21 and 59 years. Seven had prominent neck, shoulder, or respiratory muscle weakness and little or delayed ocular muscle involvement. The response to cholinesterase inhibitors was variable, and electromyographic findings suggested myopathy in several. None improved after thymectomy. All patients improved after plasma exchange, and most had a good response to selected immunotherapy. MuSK antibody status should help diagnose MG with atypical presentations and ensure appropriate patient treatment.
RsaL, a Novel Repressor of Virulence Gene Expression in Pseudomonas aeruginosa (1999)

As components of a Pseudomonas aeruginosa quorum-sensing system, LasR and PAI-1 globally regulate expression of multiple virulence determinants, as well as the second P. aeruginosa quorum-sensing system. To date, no information exists on negative regulation of the quorum-sensing cascade in P. aeruginosa. Here we describe a novel gene, rsaL, which is located downstream from lasR and transcribed antisense relative to lasR. In P. aeruginosa, overexpression of rsaL results in reduced lasB expression and decreased elastase activity. With the use of a six-His protein fusion system, we demonstrate that rsaL encodes an 11-kDa protein. Direct quantitation of PAI-1 levels in cultures and studies utilizing Escherichia coli lambda lysogens carrying lacZ transcriptional fusions reveal that RsaL specifically represses transcription of the PAI-1 autoinducer synthase gene, lasI. RsaL's repressive effect on lasI and the associated decrease in elastase activity have important implications for the expression of all LasR-PAI-1-dependent virulence genes and the overall pathogenicity of P. aeruginosa.
Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate (2014)

The erythroid stress cytokine erythropoietin (Epo) supports the development of committed erythroid progenitors, but its ability to act on upstream, multipotent cells remains to be established. We observe that high systemic levels of Epo reprogram the transcriptomes of multi- and bipotent hematopoietic stem/progenitor cells in vivo. This induces erythroid lineage bias at all lineage bifurcations known to exist between hematopoietic stem cells (HSCs) and committed erythroid progenitors, leading to increased erythroid and decreased myeloid HSC output. Epo, therefore, has a lineage instructive role in vivo, through suppression of non-erythroid fate options, demonstrating the ability of a cytokine to systematically bias successive lineage choices in favor of the generation of a specific cell type.
IP-based protocols for mobile internetworking (1991)

article Free Access Share on IP-based protocols for mobile internetworking Authors: John Ioannidis Department of Computer Science, Columbia University Department of Computer Science, Columbia UniversityView Profile , Dan Duchamp Department of Computer Science, Columbia University Department of Computer Science, Columbia UniversityView Profile , Gerald Q. Maguire Department of Computer Science, Columbia University Department of Computer Science, Columbia UniversityView Profile Authors Info & Claims ACM SIGCOMM Computer Communication ReviewVolume 21Issue 4Sept. 1991pp 235–245https://doi.org/10.1145/115994.116014Published:01 August 1991Publication History 287citation1,446DownloadsMetricsTotal Citations287Total Downloads1,446Last 12 Months84Last 6 weeks5 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Internet video category recognition (2008)

In this paper, we examine the problem of internet video categorization. Specifically, we explore the representation of a video as a ldquobag of wordsrdquo using various combinations of spatial and temporal descriptors. The descriptors incorporate both spatial and temporal gradients as well as optical flow information. We achieve state-of-the-art results on a standard human activity recognition database and demonstrate promising category recognition performance on two new databases of approximately 1000 and 1500 online user-submitted videos, which we will be making available to the community.
Self-disinfecting plastics for intravenous catheters and prosthetic inserts (1986)

A disinfectant (2,4,4'-trichloro-2'-hydroxydiphenyl ether: Irgasan, Ciba-Geigy) was incorporated into plastic washers fabricated from ethylvinyl acetate (EVA), polyethylene, polypropylene or TPX. Plastics containing 0.2 and 2% Irgasan gave zones of inhibition on nutrient and blood agar plates seeded with micro-organisms (Staphylococcus aureus, Staph. epidermidis, Escherichia coli, Proteus mirabilis or Candida albicans) even after thorough washing. Exceptionally, C. albicans was inhibited only by 2% Irgasan, and EVA gave good inhibition only against the staphylococci. Similar washers of each plastic were implanted subcutaneously into the flanks of rabbits; before insertion each was washed, had thread woven into it and was surrounded by a plasma clot containing 2 X 10(8) Staph. aureus. All the plastics without Irgasan gave rise to abscesses, none of the plastics impregnated with 2% Irgasan did, though from 2 out of 12 sites small numbers of Staph. aureus were isolated at post mortem. Using either clinical or bacteriological criteria, the results were highly significant (P less than 0.00001 and P less than 0.001 respectively), demonstrating the effectiveness of this technique in preventing plastic-associated infection.
Interaction of rat kidney P-glycoprotein with a urinary component and various drugs including cyclosporin A (1994)

The interaction of rat renal P-glycoprotein with various drugs and a hydrophobic component found in rat urine was studied to gain an understanding of both its transport function in kidney and its potential role in drug secretion and drug-induced nephrotoxicity. Rat kidney brush-border membranes (BBM) were photolabeled with [3H]azidopine, a calcium-channel blocker that covalently labeles P-glycoprotein. P-glycoprotein was immunoprecipitated with a rabbit polyclonal antibody against the human MDR1 protein (multidrug resistance gene class 1). The amount of [3H]azidopine incorporated into P-glycoprotein was quantitated following gel electrophoresis and fluorography. Photolabeling inhibition assays were conducted with a panel of drugs known to interact with P-glycoprotein in multidrug-resistant cells. Verapamil or quinidine [half-maximal inhibition constant (K0.5) = 1 microM], vinblastine (K0.5 = 3 microM), and doxorubicin or daunomycin (K0.5 = 10 microM) all blocked [3H]azidopine photolabeling of renal P-glycoprotein. Of the drugs tested, the immunosuppressant drug, cyclosporin A, interacted with kidney P-glycoprotein with the highest affinity (K0.5 = 50 nM). However, the cardiac glycoside, digoxin, failed to inhibit P-glycoprotein photolabeling. A hydrophobic rat urine extract prepared by reverse-phase chromatography also blocked photolabeling of renal P-glycoprotein. Our current hypothesis is that various drugs may inhibit urinary excretion of an endogenous substrate by virtue of their ability to bind with high affinity to P-glycoprotein. A hypothesis of drug-induced nephrotoxicity based on the interaction of various compounds like cyclosporin A with P-glycoprotein is presented.
Reaction of Imidazoles with Cyanogen Bromide: Cyanation at N 1 or Bromination at C 2? (1999)

The reaction in acetonitrile solution of a number of imidazoles(1 H -, 1-methyl-, 2-methyl-, 4-methyl-, 1,2-, 1,4- and1,5-dimethyl-, 1-ethyl-, 1-benzyl- and 1-butyl-imidazole) and imidazolecomplexes([Co(NH3)5(imH)](ClO4)3,[Co(NH3)5(im)](ClO4)2 and[Co(NH3)5(1-Meim)](ClO4)3) with BrCN has beenstudied. Those imidazoles bearing an N-alkyl substituent and having a hydrogenat C2 react to give the 2-bromo products, while the N-H imidazoles react togive N -cyano derivatives. The product(s) from thereaction of 1,2-dimethylimidazole with BrCN could not be characterized. Of thecomplexes, only[Co(NH3)5(im)](ClO4)2 reacts, giving the 2-bromoproduct. Our observations suggest a lone pair on a ring nitrogen atom isnecessary for an imidazole to react with BrCN, and a possible mechanism issuggested. The X-ray structure of 2-methylimidazole-1-carbonitrile isreported. Crystal data (–143°C) forC5H5N3:monoclinic, P 21/ c , a 10·201(5), b 7·110(3), c 7·227(3) Å, ß 100·47(2)°, V 515·4(4) Å 3 , Z 4, d calcd 1·380 gcm¯3 . Refinement of the structure converged with R 1 0·0444 for 1183reflections with F o &amp;gt;4 F ( F o) and wR 2 0·1259 for all 1278data.
A resonant inverter for electronic ballast applications (1994)

Electronic ballasts must provide enough open circuit voltage to start the fluorescent lamp and current limiting while the lamp is running. Resonant inverters may be utilized in electronic ballasts because of their load-dependent characteristics. The three basic types of resonant inverters, the series-loaded, parallel-loaded, and the series-parallel-loaded, are compared using fundamental approximation techniques for their applicability in electronic ballasts operating from a low voltage source. A parallel-loaded resonant inverter operating slightly above its resonant frequency is selected because of the high voltage gains possible. Operation above the resonant frequency allows zero-voltage turn on of the semiconductor devices. Zero-voltage turn off can be achieved with the addition of lossless snubber capacitors. Experimental results from a lab prototype are used to verify the design procedure.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Issues in Control of a Robotic Spatial Augmented Reality System (2011)

A robotic spatial augmented reality (RSAR) system combines a robotics technology with a spatial augmented reality system (SAR) where cameras are used to recognize real objects and projectors augment information and user interface directly on the surface of the recognized objects, rather than relying on handheld display devices. Moreover, a robotic module is actively used to discover and utilize the context of users and environments. The control of a RSAR system involves several issues from different technical fields such as classical inverse kinematics of motors where projector-camera pairs are mounted, inverse projection problems to find appropriate internal/external parameters of projectors and cameras, and image warping in graphics pipeline to compensate the kinematic constraints. In this paper, we investigate various control issues related to a RSAR system and propose basic approaches to handle them, specially focused on the prototype RSAR system developed in ETRI.
Drug Targeting with Monoclonal Antibodies: A review (1992)

Monoclonal antibodies have been widely used in attempts to target anti-neoplastic agents selectively to tumours. Problems associated with the use of monoclonal antibodies as the targeting moiety include lack of complete tumour selectivity, antigenic heterogeneity, tumour access and immunogenicity. Considerable effort in the targeting field is being expended in an attempt to reduce or overcome these problems. Attachment of monoclonal antibodies to low molecular weight cytotoxic drugs, protein toxins, radionuclides or enzymes capable of conversion of inactive prodrugs to cytotoxic drugs, has, despite these problems, resulted in conjugates which do have selective anti-tumour effects in animal models. The advantages and limitations of these different approaches are reviewed. It remains to be established in man if any of these approaches will result in significant therapeutic benefit in major solid tumours.
Sudden‐onset Severe Acute Asthma: Clinical Features and Response to Therapy (1998)

These data suggest that different triggers may be involved in sudden-onset SAA and that sudden onset of symptoms is independently associated with rapid recovery. In their rapid deterioration and rapid response, these subjects share certain characteristics with "sudden asphyxic asthmatics" and may constitute a population suitable for further study of factors contributing to that condition. While these visits led to admission less frequently, prospective studies are necessary to provide information on duration of response and risk for relapse.
A general algorithm to compute multilocus genotype frequencies under various mating systems (1996)

International audience
Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level (1995)

A carotenoid biosynthesis gene cluster for the production of astaxanthin was isolated from the marine bacterium Agrobacterium aurantiacum. This cluster contained five carotenogenic genes with the same orientation, which were designated crtW, crtZ, crtY, crtI, and crtB. The stop codons of individual crt genes except for crtB overlapped the start codons of the following crt genes. Escherichia coli transformants carrying the Erwinia uredovora carotenoid biosynthesis genes provide suitable substrates for carotenoid biosynthesis. The functions of the five crt genes of A. aurantiacum were determined through chromatographic and spectroscopic analyses of the pigments accumulated in some E. coli transformants carrying various combinations of the E. uredovora and A. aurantiacum carotenogenic genes. As a result, the astaxanthin biosynthetic pathway is proposed for the first time at the level of the biosynthesis genes. The crtW and crtZ gene products, which mediated the oxygenation reactions from beta-carotene to astaxanthin, were found to have low substrate specificity. This allowed the production of many presumed intermediates of astaxanthin, i.e., adonixanthin, phoenicoxanthin (adonirubin), canthaxanthin, 3'-hydroxyechinenone, and 3-hydroxyechinenone.
A recombinant trimeric surfactant protein D carbohydrate recognition domain inhibits respiratory syncytial virus infection in vitro and in vivo (1999)

The pulmonary collectin, lung surfactant protein D (SP-D), plays a role in host defense mediated by the interaction of surface carbohydrates of inhaled pathogens with the lectin domains of SP-D. Respiratory syncytial virus (RSV), the most important viral pathogen of neonates and infants, encodes a highly glycosylated attachment protein, G. Binding studies were performed with G protein from RSV (human, A2 strain) and both native and recombinant human SP-D. The effect of recombinant trimeric SP-D lectin domains (rSP-D) on the interaction between RSV and host cells was determined by two methods: an infectivity study with monolayers of Hep-2C cells and in vivo infections in BALB / c mice. These studies show that full-length and recombinant SP-D bind to RSV G protein in a concentration-dependent manner. Both EDTA and mannan inhibited binding of full-length SP-D. These results indicate that binding occurs via the carbohydrate recognition domain of the SP-D. The recombinant SP-D inhibited RSV infectivity in cell culture in a dose-dependent manner, giving 100 % inhibition of replication. Intranasal administration of recombinant SP-D to RSV-infected mice inhibited replication of the virus in the lungs, reducing levels of lung virus by 80 %. These results suggest that SP-D plays a major role in clearing RSV from the lungs.
The difference between "open-ended projects" and "design projects" (2002)

There is a difference between "engineering design projects" and good "open-ended engineering projects". There is a lot of confusion about the difference between design and an engineering project. Both types of project will follow problem solving steps. However, the "engineering design project" must also have done some conceptual design, some embodiment design and some detail design (Pahl and Beitz, 1988) or it will not be a design project. This is not to say that a design project is better or worse than an open-ended project but they are different and require different skills. Engineers will do many of both kinds of projects during their careers. It is important that we, as faculty, recognize the difference when we structure the learning experiences for our students. This paper discusses some of the design structures and gives an example of both "design projects" and a good "open-ended" engineering project.
Pattern analysis of microtubule-polymerizing and -depolymerizing agent combinations as cancer chemotherapies (2007)

Subcellular distribution of mass can be analyzed by a technique that involves culturing cells on interferometers and digitizing their interference contours. Contour sampling resulted in 102 variables per cell, which were predictors of oncogenic transformation. Cell phenotypes can be deconstructed by use of latent factors, which represent the covariance of the real variables. The reversal of the cancer-type phenotype by a combination of microtubule-stabilizing and -depolymerizing agents was described previously. The implications of these results have been explored by clinicians who treated patients with the combination of docetaxel and vinorelbine (Navelbine). The current study was performed to determine the effects of different combinations on phenotype and in phases of the cell cycle other than mitosis. Combinations of paclitaxel with either colchicine, podophyllotoxin, nocodazole, or vinblastine caused phenotype reversal. Paclitaxel analogue, 7-deoxytaxol, by itself caused reversal. Factors #4, (filopodia), #5 (displacement and/or deep invaginations in the periphery), #8, and #12 took on values typical of normal cells, whereas the values of #7 (p21-activated kinase), and #13 (rounding up) shifted toward the cancer-type. All combinations altered microtubule arrangement at the cell edge. Delivery schedules and drug ratios used in clinical studies were subjected to analysis. Clinical response rates were better when the combination was not interspersed with a single agent (P=0.004). The results support the idea that efficacy depends upon simultaneous exposure to both agents, and suggest a novel mechanism for combination therapies. These therapies appear to restore in transformed cells some of the features of a contact-inhibited cell, and to impede progress through the cell cycle even when provided at nanomolar concentrations.
Stem Cell Marker Nestin Is Critical for TGF-β1-Mediated Tumor Progression in Pancreatic Cancer (2013)

The stem cell marker nestin is an intermediate filament protein that plays an important role in cell integrity, migration, and differentiation. Nestin expression occurs in approximately one third of pancreatic ductal adenocarcinoma (PDAC), and its expression strongly correlates with tumor staging and metastasis. Little is known about the mechanisms by which nestin influences PDAC progression. Here, nestin overexpression in PDAC cells increased cell motility and drove phenotypic changes associated with the epithelial-mesenchymal transition (EMT) in vitro; conversely, knockdown of endogenous nestin expression reduced the migration rate and reverted cells to a more epithelial phenotype. Mouse xenograft studies showed that knockdown of nestin significantly reduced tumor incidence and volume. Nestin protein expression was associated with Smad4 status in PDAC cells; hence, nestin expression might be regulated by the TGF-β1/Smad4 pathway in PDAC. We examined nestin expression after TGF-β1 treatment in human pancreatic cancer PANC-1 and PANC-1 shSmad4 cells. The TGF-β1/Smad4 pathway induced nestin protein expression in PDAC cells in a Smad4-dependent manner. Moreover, increased nestin expression caused a positive feedback regulator of the TGF-β1 signaling system. In addition, hypoxia was shown to induce nestin expression in PDAC cells, and the hypoxia-induced expression of nestin is mediated by the TGF-β1/Smad4 pathway. Finally, the antimicrotubule inhibitors, cytochalasin D and withaferin A, exhibited anti-nestin activity; these inhibitors might be potential antimetastatic drugs. Our findings uncovered a novel role of nestin in regulating TGF-β1-induced EMT. Anti-nestin therapeutics may serve as a potential treatment for PDAC metastasis.