gebhart/patrepeval huggingface dataset:
Mapping of breakpoints, and relationship to BCR-ABL RNA expression, in Philadelphia-chromosome-positive chronic myeloid leukaemia patients with a breakpoint around exon 14 (b3) of the BCR gene. (1991)

The BCR gene, on chromosome 22, is involved in the Philadelphia (Ph1) chromosome which is a characteristic cytogenetic marker of chronic myeloid leukaemia (CML). Breakpoints in CML occur within the M-bcr region (5.8 kb) which encompasses exons 12-15 (b1-b4), and the M-bcr can be conveniently divided into five zones by restriction mapping. One of these zones (3) contains exon b3 which can be either present or absent from the hybrid mRNA, even if it is present in the chimaeric gene. We have mapped the breakpoints around BCR exon b3 and related this to the type of RNA splice site expressed, in CML patients at diagnosis. Breakpoints within zone 3 were restriction mapped to one of six sub-zones and the site related to the type of RNA splice site. Two clusters of breakpoints within zone 3 were observed. One cluster was located around exon b3 and often resulted in deletion of exon b3 from the chimaeric gene. The majority of this cluster expressed b2-a2 spliced RNA, usually as a consequence of a deletion removing exon b3. The second cluster occurred within two sub-zones that spanned an Alu sequence, and 90% of this cluster exhibited b3-a2 spliced RNA. Furthermore, a greater number of patients had entered blast crisis if the RNA contained BCR exon b3 (8 of 10 patients), compared to those with b2-a2 spliced RNA (3 of 12 patients). The high degree of heterogeneity in the site of the breakpoint within zone 3 of the M-bcr, combined with the type of BCR-ABL hybrid mRNA expressed, further implicates BCR exon b3 in the pathogenesis of CML.
Transcoronary transplantation of autologous mesenchymal stem cells and endothelial progenitors into infarcted human myocardium (2005)

The aim of the study was to investigate whether a combination of mesenchymal stem cells (MSCs) capable of differentiating into cardiac myocytes and endothelial progenitors (EPCs) that mainly promote neoangiogenesis might be able to facilitate tissue repair in myocardial scars. Previous studies have shown that intracoronary transplantation of autologous bone marrow stem cells results in improvement of contractility in infracted areas of human myocardium. Eleven patients with an anteroseptal myocardial infarction (MI) underwent transcoronary transplantation of bone marrow-derived MSCs and EPCs to the infarcted area through the left anterior descending artery. Eleven age- and sex-matched patients served as controls. Wall motion score index was significantly lower at follow-up in the transplantation (P = 0.04) but not in the control group. On stress echocardiography, there was improvement of myocardial contractility in one or more previously nonviable myocardial segments in 5 out of 11 patients (all with recent infarctions) and in none of the controls (P = 0.01). Restoration of uptake of Tc(99m) sestamibi in one or more previously nonviable myocardial scars was seen in 6 out of 11 patients subjected to transplantation and in none of the controls (P = 0.02). Cell transplantation was an independent predictor of improvement of nonviable tissue. Intracoronary transplantation of MSCs and EPCs is feasible, safe, and may contribute to regional regeneration of myocardial tissue early or late following MI.
FGFR2 -Amplified Gastric Cancer Cell Lines Require FGFR2 and Erbb3 Signaling for Growth and Survival (2008)

We have identified a critical role for amplified FGFR2 in gastric cancer cell proliferation and survival. In a panel of gastric cancer cell lines, fibroblast growth factor receptor 2 (FGFR2) was overexpressed and tyrosine phosphorylated selectively in FGFR2-amplified cell lines KatoIII, Snu16, and OCUM-2M. FGFR2 kinase inhibition by a specific small-molecule inhibitor resulted in selective and potent growth inhibition in FGFR2-amplified cell lines, resulting in growth arrest in KatoIII cells and prominent induction of apoptosis in both Snu16 and OCUM-2M cells. FGFR2-amplified cell lines also contained elevated phosphotyrosine in EGFR, Her2, and Erbb3, but the elevated phosphorylation in EGFR could not be inhibited by gefitinib or erlotinib. We show that the elevated EGFR, Her2, and Erbb3 phosphotyrosine is dependent on FGFR2, revealing EGFR family kinases to be downstream targets of amplified FGFR2. Moreover, shRNA to Erbb3 resulted in a loss of proliferation, confirming a functional role for the activated EGFR signaling pathway. These results reveal that both the FGFR2 and EGFR family signaling pathways are activated in FGFR2-amplified gastric cancer cell lines to drive cell proliferation and survival. Inhibitors of FGFR2 or Erbb3 signaling may have therapeutic efficacy in the subset of gastric cancers containing FGFR2 amplification.
Simple Bargaining Agents for Decentralized Market-Based Control (1998)

market-based control, economic agents, bargaining, autonomous agent Market-Based Control (MBC) is a resource allocation and control technique where multi-agent systems are built to resemble free-market economies. The aim is that MBC systems exhibit the same decentralization, robustness, and capacity for self-organization as do real economies. MBC systems are relevant to Artificial Intelligence (AI) and robotics in at least two ways: first, the agents in a MBC system need to be robot-like in their ability to autonomously coordinate perception and action in dynamic and uncertain environments that include other agents; second, MBC systems could be used as the control technologies for robots and other “intelligent” autonomous agents. We critically review a selection of MBC systems. We argue that the MBC systems reviewed here are either implicitly reliant on centralized knowledge, or require human operators and hence are not truly automatic. We identify a major issue in creating truly decentralized and automatic MBC systems: the need for the system's agents to be capable of bargaining behaviors. Following this, we briefly summarize our current results and ongoing work in creating multi-agent systems where each autonomous agent has the ability to bargain with other agents. We demonstrate that markets composed of such agents exhibit desirable behaviors, and that such agents could form the basis of truly decentralized MBC systems.
Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii (2008)

The opportunistic human pathogen Acinetobacter baumannii strain M2 was found to produce distinct acyl-homoserine lactone (AHL) signals based on the use of an Agrobacterium tumefaciens traG-lacZ biosensor. An A. baumannii gene, designated abaI, was cloned and directed AHL production in recombinant Escherichia coli. The AbaI protein was similar to members of the LuxI family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by A. baumannii. The primary AHL signal directed by AbaI was identified by mass spectrometry as being N-(3-hydroxydodecanoyl)-L-HSL (3-hydroxy-C(12)-HSL). Minor amounts of at least five additional AHLs were also identified. The expression of abaI at the transcriptional level was activated by ethyl acetate extracts of culture supernatants or by synthetic 3-hydroxy-C(12)-HSL. An abaI::Km mutant failed to produce any detectable AHL signals and was impaired in biofilm development.
Tumor necrosis factor receptor signaling. A dominant negative mutation suppresses the activation of the 55-kDa tumor necrosis factor receptor. (1992)

To investigate the signaling mechanism of the 55-kDa tumor necrosis factor (TNF) receptor a functional transfection based assay was developed. The human 55-kDa TNF receptor, stably expressed in mouse L929 cells, was demonstrated to be activated specifically by agonist antibodies and to initiate a signal for cellular cytotoxicity. A deletion mutant of the human TNF receptor lacking most of the cytoplasmic domain was found to be completely defective in generating the signal for cytotoxicity. Additionally, expression of the truncated receptor substantially suppressed signaling by endogenous mouse TNF receptors in response to TNF, but not in response to specific anti-murine TNF receptor antibodies. These results suggest that aggregation of 55-kDa TNF receptor intracellular domains, which are not associated in the absence of ligand, is an important component of the signal for cellular toxicity. This work also provides an example of a dominant negative mutation in a transmembrane receptor that lacks a tyrosine kinase domain, and suggests a more general utility of dominant negative mutations in the investigation of cytokine receptor function.
Emergency Medical Services and Sudden Cardiac Arrest: The "Chain of Survival" Concept (1993)

Early access to the EMS system helps insure early CPR, defibrillation, and advanced care. Early access is easiest to achieve with 911 systems and widespread community education and publicity. It may also be taught during citizen-CPR classes. Early CPR helps patients by slowing the process of dying, but its effectiveness disappears within minutes, and defibrillation must soon follow. A citizenry well-informed about cardiac emergencies and well-trained in CPR appears to be the best method to achieve early recognition and early CPR. The earliest possible delivery of defibrillation is critical and, almost by itself, sufficient for many sudden death cases. It has, therefore, emerged as the single most effective intervention for patients in nontraumatic cardiac arrest. Automated external defibrillators help accomplish this goal and now permit widespread implementation of a variety of early defibrillation programs. Early advanced care helps those who do not immediately convert to an organized cardiac activity or who do not achieve a spontaneous circulation following early defibrillation. Advanced care allows a system to approach its highest possible survival rate by respiratory and anti-arrhythmic stabilization and monitoring of patients in the post-resuscitation period. At present, early CPR and rapid defibrillation, combined with early advanced care, can result in long-term survival rates as high as 30% for witnessed VF. Because neurological and psychological recovery from cardiac arrest are tied to the time within which these critical interdependent treatment modalities are delivered (1, 87), high resuscitation rates will also lead to a high percentage of patients who recover to their pre-arrest neurologic level. The public health challenge is to develop programs that will allow recognition, access, bystander-CPR, defibrillation, and advanced care to be delivered as quickly as possible, ideally within moments of the collapse of a sudden death victim. Achievement of such a goal requires the deployment of multiple properly directed programs within an EMS system; each program lends strength to the chain of survival, thereby enhancing successful recovery and long-term survival. What benefits would occur if a majority of EMS systems in the United States could establish cost-effective programs with respectable survival rates? The AHA estimates that full implementation of potential life-saving mechanisms in the community may save 10,000-100,000 lives each year in the US (2). If the maximum survival rate for all nontraumatic cardiac arrests in mature EMS systems is about 20% (33) among the annual 400,000 out-of-hospital cardiac arrests, 80,000 persons would be saved (33).(ABSTRACT TRUNCATED AT 400 WORDS)
Portable Rapid Visual Workflow Simulation Tool for Human Robot Coproduction (2017)

Within the European Factory-in-a-day project, the aim is to improve communication between automation integrator and factory owner, in their analysis of feasibility and appropriateness of automating a manual task. A visualisation tool with preconfigured workflows and working principles, with specific focus on efficient human-robot coproduction workflows can improve this process. This paper describes the Workflow Simulation Tool, which is part of the Human-Robot Coproduction Methodology, currently in development. The tool encompasses a portable tablet PC, which runs a visual modelling environment combined with a handheld 3D scanning solution. The tool also features pre-modelled template layouts, implementation of a checklist of persistent notes and portable visual documentation. The tool's appropriateness was iteratively validated in collaboration with automation integrators. This evaluation showed that offering an interactive visual simulation enriches the dialogue during conceptual design and helps in revealing requirements that otherwise only appear during or after implementation.
Concentration-specific hydrogen sensing behavior in monosized Pd nanoparticle layers (2008)

In this study, H2 sensing behavior of monosized and monocrystalline Pd nanoparticles has been studied as a function of H2 concentration and measurement temperature. A unique concentration-specific H2 sensing behavior with a 'pulsed' response at larger H2 concentrations and 'saturated' response at lower concentrations has been observed. The threshold concentration required for transition from 'saturated' to 'pulsed' response is very sensitive to measurement temperature. The characteristic change in the sensing behavior can be used to develop a novel sensor capable of determining H2 concentration level having high sensitivity and fast response. This study demonstrates that electrical and gas sensing properties of the nanoparticle layer depend critically on interparticle gaps.
A Hardware Platform for Utilizing TV Bands With a Wi-Fi Radio (2007)

The Federal Communications Commission (FCC) is currently exploring the use of TV bands for unlicensed communication. This step has sparked significant interest in the research and corporate community as it opens up new possibilities for high speed and long range wireless communication. In this paper, we present the design and implementation of a complete system that can detect the presence of TV signals, and perform high-speed data communication in an available TV band without interfering with neighboring TV bands. To the best of our knowledge, this is the first known system with all the above capabilities.
Physical properties of a complete volume-limited sample of Ap/Bp stars: Magnetic. rotational, multiplicity, and evolutionary properties of magnetic intermediate-mass stars within 100 pc of the sun (2007)

Thesis (Master, Physics, Engineering Physics and Astronomy) -- Queen's University, 2007-09-20 09:23:09.29
A novel p16INK4A transcript. (1995)

p16INK4A and p15INK4B were initially identified as potent inhibitors of activated cyclin/cyclin-dependent kinase complexes. These genes were colocalized to chromosome 9p21, and p16 was subsequently found to be mutated in familial melanoma and deleted in a wide variety of sporadic cancers. We recently found that de novo methylation of a 5' CpG island led to transcriptional block of full-length p16 in many neoplasms. However, the presence of a truncated p16 transcript in methylated cell lines led us to investigate the presence of an alternative promoter or initiation site. We have now identified an abundant alternative p16 transcript in both methylated and unmethylated cell lines generated from a novel sequence (exon 1 beta) potentially involved in the complex regulation of these critical cell cycle genes.
The Action of Formaldehyde on Amines and Amino Acids1 (1933)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Action of Formaldehyde on Amines and Amino Acids1H. T. Clarke, H. B. Gillespie, and S. Z. WeisshausCite this: J. Am. Chem. Soc. 1933, 55, 11, 4571–4587Publication Date (Print):November 1, 1933Publication History Published online1 May 2002Published inissue 1 November 1933https://pubs.acs.org/doi/10.1021/ja01338a041https://doi.org/10.1021/ja01338a041research-articleACS PublicationsRequest reuse permissionsArticle Views6723Altmetric-Citations439LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of the retinal-binding protein in halorhodopsin. (1982)

Cell envelope vesicles prepared from a retinal-deficient strain of Halobacterium halobium contained the apoprotein of halorhodopsin, but not the apoprotein of bacteriorhodopsin. Halorhodopsin was reconstituted in these membranes with tritium-labeled retinal and the preparation was reduced with sodium cyanoborohydride. The product was a bleached pigment in which the retinal-protein bond was resistant to hydroxylamine cleavage. Fluorography of sodium dodecyl sulfate/urea-polyacrylamide gels showed that one protein was radioactively labeled, almost exclusively. This protein migrated with an apparent molecular weight somewhat lower than that of bacteriorhodopsin, which was reconstituted in membranes from another strain and radioactively labeled under the same conditions. Thus, the retinal-binding component of halorhodopsin is a small protein, with an apparent molecular weight of approximately 25,000.
Effect of radiotherapy delay in overall treatment time on local control and survival in head and neck cancer: Review of the literature (2015)

Treatment delays in completing radiotherapy (RT) for many neoplasms are a major problem affecting treatment outcome, as increasingly shown in the literature. Overall treatment time (OTT) could be a critical predictor of local tumor control and/or survival. In an attempt to establish a protocol for managing delays during RT, especially for heavily overloaded units, we have extensively reviewed the available literature on head and neck cancer. We confirmed a large deleterious effect of prolonged OTT on both local control and survival of these patients.
Mutagenesis of the Glu-89 residue in human immunodeficiency virus type 1 (HIV-1) and HIV-2 reverse transcriptases: effects on nucleoside analog resistance (1992)

A Glu-89-->Gly alteration in the human immunodeficiency virus type 1 reverse transcriptase (RT) was previously shown to result in resistance to several dideoxynucleoside analogs and to phosphonoformic acid (PFA; foscarnet). This residue was altered to Ala, Val, Ser, Thr, Gln, Asp, Asn, or Lys, and the ddGTP and PFA sensitivities of the mutant RTs were measured. Replacements with Ala, Gly, Val, and Thr led to resistance to inhibition by ddGTP, while mutants with amino acid Ser, Gln, Asn, Asp, or Lys displayed only moderate or no resistance. A similar result was obtained with inhibition by PFA, except that the Asp-89 mutant also displayed resistance. Furthermore, the introduction of Glu-89-->Gly alteration into the RT of human immunodeficiency virus type 2 likewise rendered it resistant to both ddGTP and PFA.
The GPU Computing Era (2010)

GPU computing is at a tipping point, becoming more widely used in demanding consumer applications and high-performance computing. This article describes the rapid evolution of GPU architectures-from graphics processors to massively parallel many-core multiprocessors, recent developments in GPU computing architectures, and how the enthusiastic adoption of CPU+GPU coprocessing is accelerating parallel applications.
Miniaturized capillary isoelectric focusing in plastic microfluidic devices (2002)

We report the demonstration of miniaturized capillary isoelectric focusing (CIEF) in plastic microfluidic devices. Conventional CIEF technique was adapted to the microfluidic devices to separate proteins and to detect protein-protein interactions. Both acidic and basic proteins with isoelectric points (pI) ranging from 5.4 to 11.0 were rapidly focused, mobilized, and detected in a 1.2 cm long channel (50 microm deep x 120 microm wide) with a total analysis time of 150 s. In a device with a focusing distance of 4.7 cm, the separation efficiency for a basic protein, lysozyme, was achieved as high as 1.5 x 10(5) plates, corresponding to 3.2 million plates per meter. We also experimentally confirmed that IEF resolution is essentially independent of focusing length when the applied voltage is kept the same and within a range that it does not cause Joule heating. Further, we demonstrated the use of miniaturized CIEF to study the interactions between two pairs of proteins, immunoglobulin G (IgG) with protein G and anti-six histidine (anti-6xHis) with 6xHis-tagged green fluorescent protein (GFP). Using this approach, protein-protein interactions can be detected for as little as 50 fmol of protein. We believe miniaturized CIEF is useful for studying protein-protein interactions when there is a difference in pI between a protein-protein complex and its constitutent proteins.
A Review of Charge Scheduling of Electric Vehicles in Smart Grid (2014)

Electric vehicles (EVs) are being introduced by different manufacturers as an environment-friendly alternative to vehicles with internal combustion engines, with several benefits. The number of EVs is expected to grow rapidly in the coming years. However, uncoordinated charging of these vehicles can put a severe stress on the power grid. The problem of charge scheduling of EVs is an important and challenging problem and has seen significant research activity in the last few years. This review covers the recent works done in the area of scheduling algorithms for charging EVs in smart grid. The works are first classified into two broad classes of unidirectional versus bidirectional charging, and then, each class is further classified based on whether the scheduling is centralized or distributed and whether any mobility aspects are considered or not. It then reviews the key results in this field following the classification proposed. Some interesting research challenges that can be addressed are also identified.
Escherichia coli Sequence Type ST131 as an Emerging Fluoroquinolone-Resistant Uropathogen among Renal Transplant Recipients (2009)

Among 40 Escherichia coli urine isolates from renal transplant recipients (Galveston, TX, 2003 to 2005), sequence type ST131 (O25:H4) was highly prevalent (representing 35% of isolates overall and 60% of fluoroquinolone-resistant isolates), virulent appearing, antimicrobial resistant (but extended-spectrum-cephalosporin susceptible), and associated with black race. Pulsotypes were diverse; some were linked to other locales. ST131 emerged significantly during the study period. These findings suggest that E. coli ST131 may constitute an important new multidrug-resistant threat to renal transplant recipients.