gebhart/patrepeval huggingface dataset:
Intravenous Administration of S-ketamine in a Severely Depressed Treatment-resistant Patient Receiving Tranylcypromine: a Case Report (2015)

Tranylcypromine, an irreversible monoamine-oxidase (MAO) inhibitor, has been frequently used to treat the most challenging mood disorders since 1950s. Due to dietary restrictions as well as feared drug interactions, tranylcypromine has been increasingly replaced by modern antidepressants lacking such challenging pharmacology. Ketamine, a high-affinity non-competitive antagonist at the N-methyl-D-aspartate receptor, has been increasingly appreciated as rapid-acting antidepressant with good anti-suicidal properties. Previous in-vitro and ex-vivo studies suggested that ketamine acts as a monoamine-reuptake inhibitor. Since inhibition of monoamine-reuptake with concurrent blockade of MAO should have the potential to precipitate a sympathomimetic crisis, a combination of ketamine and tranylcypromine is considered hazardous. Here we report on a 43-year-old, 120 kg female patient suffering from treatment-resistant depression with recurrent severe suicidal crises, who was treated with tranylcypromine. Because of elective surgery requiring general anaesthesia, tranylcypromine was discontinued for two weeks. After reinstating tranylcypromine 10 mg p.o.q.d., she became acutely suicidal again. Hence, intravenous S-ketamine 12.5 mg was slowly administered under cardiovascular monitoring. We observed good anti-suicidal effects, while no relevant changes in blood pressure or heart rate occurred during or after s-ketamine infusion. Under plasma level monitoring tranylcypromine was increased to 80 mg p.o.q.d., and intravenous S-ketamine up to 75 mg was repeatedly administered. This report is believed to be the first to demonstrate repeated concomitant administration of S-ketamine and tranylcypromine as antidepressant treatment in humans lacking any relevant changes in cardiovascular parameters. Hence, we put serious doubt on whether monoamine-reuptake inhibition is a relevant pharmacological effect of ketamine in humans.
Sialyl Lewisx (sLex) and an sLexMimetic, CGP69669A, Disrupt E-Selectin–Dependent Leukocyte Rolling In Vivo (1998)

Leukocyte rolling is the earliest observable event in their recruitment from the circulation to inflamed tissue. This rolling is mediated largely by interaction between the selectin family of adhesion molecules and their glycosylated ligands. Although the nature of these ligands and their interaction with the selectins is not fully understood, it is accepted that expression of fucosylated sialylated glycans such as sialyl Lewisx (sLex) is required for function. Despite findings that sLex inhibits binding of leukocytes to E-selectin in vitro, and has beneficial effects in inflammatory disease models, inhibition of E-selectin–dependent leukocyte rolling in vivo has not been described. Functional overlap between the selectins has been noted and reduction of rolling by E-selectin antibodies only occurs if P-selectin is absent or blocked. We demonstrate that leukocyte rolling velocity in tumor necrosis factor alpha (TNFα)-stimulated mouse cremaster is increased following treatment with either sLex or the sLex-mimetic CGP69669A and that rolling is dramatically reduced if CGP69669A is applied in the presence of anti–P-selectin antibody. These effects are characteristic of E-selectin antagonism. In contrast, surgically stimulated (L- or P-selectin–dependent) rolling is unaffected by either sLex or CGP69669A. Our data demonstrate that CGP69669A is an effective and selective antagonist of E-selectin in vivo.
Comparative Evaluation of Viability of Encapsulated Lactobacillus casei Using Two Different Methods of Microencapsulation (2014)

Microencapsulation using two different methods, spray- drying and emulsion technique were applied to preserve the viability of the probiotic Lactobacillus casei during manufacture and refrigerated storage. As coating materials to encapsulate the probiotic by spray-drying method, compatible biopolymers alginate and chitosan were utilized, while as a cross-linking agent, CaCl2 was used. In addition to the probiotic, oligofructose enriched inulin (Synergy 1®) as prebiotic was added to the medium intended for spray-drying. For microencapsulation of the probiotic by emulsion method, alginate and whey proteins were applied. Further, protective effects of four potential cryoprotectants (oligofructose enriched inulin, sorbitol, sucrose, lactose) were investigated when added to the whey proteins-Ca-alginate microparticles before freeze-drying. Experiments showed that chitosan-Ca-alginate microparticles and whey protein-Ca-alginate microparticles with high viability of L. casei were obtained using spray-drying and emulsion method, respectively. Lactobacillus casei encapsulated by emulsion method survived better during 6-months storage at refrigerated conditions than L. casei encapsulated by spraydrying.
Cryoprotectants have no additional effect on the cells survival during freeze-drying, while sorbitol has shown negative impact on the probiotic viability encapsulated in whey protein-alginate and subsequently freeze-dried. No significant differences in viability of L. casei during storage at refrigerated conditions were observed, both in whey proteins-Ca-alginate microparticles as control and formulations containing cryoprotective agent.
Vasoactive intestinal peptide hydrolysis by antibody light chains (1991)

This paper describes evidence for hydrolysis of a neuropeptide, vasoactive intestinal peptide (VIP), by light chains purified from the IgG of a human subject positive for VIP binding antibodies. Purified IgG was digested with papain, resultant fragment antigen binding (Fab) fragments were reduced with 2-mercaptoethanol and alkylated with iodoacetamide, and light chains were purified by chromatography on immobilized antibodies to light chains and immobilized antibodies to heavy chains. Non-immunoglobulin components were undetectable in the light chain preparation, judged by sodium dodecyl sulfate-electrophoresis and Western blotting with anti-heavy and anti-light chain antibodies. The light chains hydrolyzed VIP with specific activity 32-fold greater than that of Fab, the pH optimum for light chain-mediated VIP hydrolysis was 7.0-7.5, and the hydrolytic activity was saturable (Vmax, 0.19 pmol/min/microgram light chains; substrate concentration at Vmax/2,380 nM).
Lipid Binding of the Exchangeable Apolipoprotein Apolipophorin III Induces Major Changes in Fluorescence Properties of Tryptophans 115 and 130 (2000)

The effect of lipid association on the local environment of the two tryptophan residues of Locusta migratoria apolipophorin III (apoLp-III) has been studied. In the lipid-free state, Trp115 in helix 4 is buried in the hydrophobic interior of the helix bundle, while Trp130 is located in a loop connecting helices 4 and 5. Fluorescence spectroscopy of single Trp mutants revealed an emission maximum (lambda(max)) of 321 nm for apoLp-III-W@115 (excitation 280 nm) which red-shifted to 327 nm upon binding to dimyristoylphosphatidylcholine (DMPC). ApoLp-III-W@130 displayed a lambda(max) of 338 nm while interaction with DMPC resulted in a blue shift to 331 nm. Quenching studies with KI and acrylamide revealed decreased accessibility to Trp115 compared to Trp130, while lipid binding induced a decrease in quenching of Trp130. Aromatic circular dichroism (CD) spectra showed that Trp vibronic transitions at 278, 286, and 294 nm for lipid-free apoLp-III were caused by Trp115. Upon lipid association, aromatic extrema are reversed in sign, becoming entirely negative with both Trp residues contributing to the vibronic transitions, implying restriction in side-chain mobility of these residues. Thus, lambda(max), quencher accessibility, and aromatic CD analysis indicate that Trp115 is much less solvent-exposed than Trp130. Differences in fluorescence properties of these residues are minimized in the lipid-bound state, a result of relocation of Trp115 and Trp130 into the lipid milieu. Thus, in addition to the hydrophobic faces of apoLp-III amphipathic alpha-helices, the loop region containing Trp130 comes in close contact with DMPC.
Role of follow-up in management of local recurrences of colorectal cancer (1998)

Our data support use of an intense follow-up plan after primary resection of large-bowel cancer, at least in patients with rectal cancer.
A New Peptide Ligand for Targeting Human Carbonic Anhydrase IX, Identified through the Phage Display Technology (2010)

These data indicate that CaIX-P1 is a promising candidate for the development of new ligands targeting human carbonic anhydrase IX.
Applications of antenna arrays to mobile communications. I. Performance improvement, feasibility, and system considerations (1997)

The demand for wireless mobile communications services is growing at an explosive rate, with the anticipation that communication to a mobile device anywhere on the globe at all times will be available in the near future. An array of antennas mounted on vehicles, ships, aircraft, satellites, and base stations is expected to play an important role in fulfilling the increased demand of channel requirement for these services, as well as for the realization of the dream that a portable communications device the size of a wristwatch be available at an affordable cost for such services. This paper is the first of a two-part study. It provides a comprehensive treatment, at a level appropriate to nonspecialists, of the use of an antenna array to enhance the efficiency of mobile communications systems. It presents an overview of mobile communications as well as details of how an array may be used in various mobile communications systems, including land-mobile, indoor-radio, and satellite-based systems. It discusses advantages of an array of antennas in a mobile communications system, highlights improvements that are possible by using multiple antennas compared to a single antenna in a system, and provides details on the feasibility of antenna arrays for mobile communications applications.
A maintenance support system based on high-level control-flow and data dependency (2002)

We propose a new method to support the maintenance of large scale business application systems, and discuss its implementation. Large scale business applications handle huge numbers of data items. To improve the maintenance of business application systems, our method proposes integrating the following features: (1) a high level control flow diagram which displays an unstructured program in a structured form; (2) a data dependency diagram which is essential in understanding business rules; and (3) ripple effect analysis functions to find the side effects of program modification. This paper also describes an execution path model and a repository which enable us to efficiently implement the above features. The system has been developed to reflect the discussions with software maintainers.
Potential Role of High-Mobility Group Box 1 in Cystic Fibrosis Airway Disease (2008)

HMGB1 expression contributes to pulmonary inflammation and lung matrix degradation in CF airway disease and deserves further investigation as a biomarker and potential therapeutic target.
Plasma elevation of stromal cell–derived factor-1 induces mobilization of mature and immature hematopoietic progenitor and stem cells (2001)

The chemokine, stromal cell-derived factor-1 (SDF1), is produced in the bone marrow and has been shown to modulate the homing of stem cells to this site by mediating chemokinesis and chemotaxis. Therefore, it was hypothesized that elevation of SDF1 level in the peripheral circulation would result in mobilization of primitive hematopoietic stem and progenitor cells. SDF1 plasma level was increased by intravenous injection of an adenoviral vector expressing SDF1alpha (AdSDF1) into severe combined immunodeficient mice. This resulted in a 10-fold increase in leukocyte count, a 3-fold increase in platelets, and mobilization of progenitors, including colony-forming units-granulocyte-macrophage to the peripheral circulation. In addition, AdSDF1 induced mobilization of cells with stem cell potential, including colony-forming units in spleen and long-term reconstituting cells. These data demonstrate that overexpression of SDF1 in the peripheral circulation results in the mobilization of hematopoietic cells with repopulating capacity, progenitor cells, and precursor cells. These studies lay the foundation for using SDF1 to induce mobilization of hematopoietic stem and progenitor cells in in vivo studies. (Blood. 2001;97:3354-3360)
A transgenic mouse that expresses a diversity of human sequence heavy and light chain immunoglobulins (1992)

We have generated transgenic mice that express a diverse repertoire of human sequence immunoglobulins. The expression of this repertoire is directed by light and heavy chain minilocus transgenes comprised of human protein coding sequences in an unrearranged, germ-line configuration. In this paper we describe the construction of these miniloci and the composition of the CDR3 repertoire generated by the transgenic mice. The largest transgene discussed is a heavy chain minilocus that includes human mu and gamma 1 coding sequences together with their respective switch regions. It consists of a single 61 kb DNA fragment propagated in a bacterial plasmid vector. Both human heavy chain classes are expressed in animals that carry the transgene. In light chain transgenic animals the unrearranged minilocus sequences recombine to form VJ joints that use all five human J kappa segments, resulting in a diversity of human-like CDR3 regions. Similarly, in heavy chain transgenics the inserted sequences undergo VDJ joining complete with N region addition to generate a human-like VH CDR3 repertoire. All six human JH segments and at least eight of the ten transgene encoded human D segments are expressed. The transgenic animals described in this paper represent a potential source of human sequence antibodies for in vivo therapeutic applications.
Evaluation of infrared spectroscopic techniques for analysing chitosan (1985)

Abstract The use of the 3450 cm −1 absorption band as an internal standard in the determination of the extent of N ‐acetylation of chitosan from the absorbance of the amide I band at 1655 cm −1 was studied. The results show that the technique gives consistent results that are relatively unaffected by whether the sample is air‐dried or oven‐dried. A number of chitosan samples, covering the range of N ‐acetyl values from 14% to 72% were analysed by three techniques: infrared spectroscopy using the ratio A 1655 / A 3450 , hydrobromide salt titration and residual salicylaldehyde determination. There is a very good correlation between the results from infrared spectroscopy and those from the other two analytical techniques, confirming the validity of the former technique.
Distinct rat genes with related profiles of expression define a TIE receptor tyrosine kinase family. (1993)

We have isolated rat cDNAs that encode two related receptor-like tyrosine kinases. One of these receptors, TIE-1, is the rat homolog of a recently described human receptor-like kinase termed TIE (Partanen et al., 1992). The related TIE-2 receptor has the same organization of amino acid sequence motifs characteristic of TIE-1: two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin III-like repeats in the extracellular region and a short kinase insert sequence and C-terminal tail in the intracellular region. The amino acid sequences of the intracellular and extracellular regions of TIE-1 and TIE-2 are 79% and 32% identical respectively. Both tie genes are broadly expressed in embryonic, neonatal and adult tissues, accounted for largely by their coexpression in endothelial cells. The tie-2 gene is also uniquely expressed in several additional embryonic tissues, including the lens epithelium and the heart epicardium.
Helix-Forming Propensity of Aliphatic Urea Oligomers Incorporating Noncanonical Residue Substitution Patterns (2013)

Aliphatic N,N'-linked oligoureas are peptidomimetic foldamers that adopt a well-defined helical secondary structure stabilized by a collection of remote three-center H-bonds closing 12- and 14-membered pseudorings. Delineating the rules that govern helix formation depending on the nature of constituent units is of practical utility if one aims to utilize this helical fold to place side chains in a given arrangement and elaborate functional helices. In this work, we tested whether the helix geometry is compatible with alternative substitution patterns. The central -NH-CH(R)-CH2-NH-CO- residue in a model oligourea pentamer sequence was replaced by guest units bearing various substitution patterns [e.g., -NH-CH2-CH2-NH-CO-, -NH-CH2-CH(R)-NH-CO-, and -NH-CH(R(1))-CH(R(2))-NH-CO-], levels of preorganization (cyclic vs acyclic residues), and stereochemistries, and the helix formation was systematically assessed. The extent of helix perturbation or stabilization was primarily monitored in solution by Fourier transform IR, NMR, and electronic circular dichroism spectroscopies. Our results indicate that although three new substitution patterns were accommodated in the 2.5-helix, the helical urea backbone in short oligomers is particularly sensitive to variations in the residue substitution pattern (position and stereochemistry). For example, the trans-1,2-diaminocyclohexane unit was experimentally found to break the helix nucleation, but the corresponding cis unit did not. Theoretical calculations helped to rationalize these results. The conformational preferences in this series of oligoureas were also studied at high resolution by X-ray structure analyses of a representative set of modified oligomers.
Enhanced graphic matrix analysis of nucleic acid and protein sequences. (1981)

The enhanced graphic matrix procedure analyzes nucleic acid and amino acid sequences for features of possible biological interest and reveals the spatial patterns of such features. When a sequence is compared to itself the technique shows regions of self-complementarity, direct repeats, and palindromic subsequences. Comparison of two different sequences, exemplified by immunoglobulin kappa light chain genes, by using colored graphic matrices showed domains of similarity, regions of divergence, and features explainable by transpositions. Analysis of mouse constant domain immunoglobulin sequences revealed self-complementary regions that can be used to fold the molecule into a structure consistent with electron microscopic observations. Computer translation of nucleic acid sequences into all possible amino acid sequences followed by graphic matrix analysis provides a way to detect the most likely protein encoding regions and can predict the correct reading frames in sequences in which splicing patterns are not defined. Application of this technique to regions of simian virus 40 and polyoma virus demonstrates the frames of translation and shows the agreement of sequences determined in separate laboratories with different virus isolates. The graphic matrix technique can also be used to assemble fragmentary sequences during determination, to display local variations in base composition, to detect distant evolutionary relationships, and to display intragenic variation in rates of evolution.
Competition between Bactericidal/Permeability-Increasing Protein and Lipopolysaccharide-Binding Protein for Lipopolysaccharide Binding to Monocytes (1993)

The bactericidal/permeability-increasing protein (BPI) inhibits the lipopolysaccharide (LPS)-mediated activation of monocytes. Due to its inhibitory activity for various LPS, BPI has therapeutic potential in endotoxic shock. To be efficient in vivo, BPI should overcome the action of LPS-binding protein (LBP), a serum molecule that increases the expression of LPS-inducible genes via CD14 of monocytes, rBPI23, a recombinant fragment of BPI, prevented in a dose-dependent manner the binding and the internalization of LPS mediated by LBP. Consequently, rBPI23 also inhibited LPS-induced tumor necrosis factor (TNF alpha) synthesis from monocytes. LPS- and LBP-mediated activation of monocytes was totally inhibited when LPS was preincubated with rBPI23. Adding rBPI23 at the same time as LBP resulted in an important but partial inhibition of TNF alpha release, but this inhibition vanished with delaying the time of addition of rBPI23. These studies suggest that the inhibitory activity of BPI is related to its ability to compete with LBP for LPS.
Synthesis of Polyacetylenic Acids Isolated from Heisteria acuminata (2001)

Four linear polyacetylenic compounds were synthesized. Pentadeca-6,8,10-triynoic acid 1 and octadeca-8,10,12-triynoic acid 2 were synthesized by using acetylene coupling reactions. The syntheses of (Z)-hexadec-11-en-7,9-diynoic acid 3 and (Z)-octadec-12-en-7,9-diynoic acid 4 by using vinylic telluride coupling reactions were accomplished.
Interleukin-13 induces the production of interleukin-1 receptor antagonist (IL-1ra) and the expression of the mRNA for the intracellular (keratinocyte) form of IL-1ra in human myelomonocytic cells (1994)

Abstract The aim of this study was to examine the expression of interleukin-1 receptor antagonist (IL-1ra) in human myelomonocytic cells treated with IL-13. IL-13 induced IL-1ra transcripts in human circulating monocytes and polymorphonuclear cells (PMN). Induction by IL-13 was not blocked, but rather superinduced, in the presence of the protein synthesis inhibitor cycloheximide. Actinomycin D blocked induction, suggesting involvement of gene transcription. The half-life of IL-1ra transcripts was prolonged by IL-13 from 1.3 hours to 4.5 hours in monocytes and to 12 hours in PMN. By reverse transcriptase-polymerase chain reaction, IL- 13 was found to augment the transcripts coding for the soluble form of IL-1ra, but also to induce the expression of the intracellular (keratinocyte) form of IL-1ra, the latter being extremely low or undetectable in myelomonocytic cells. IL-13 induced production of IL- 1ra in myelomonocytic cells, augmenting both cell-associated and released protein. Induction of IL-1ra by IL-13 may represent a further mechanism by which this molecule can counteract the potent proinflammatory properties of IL-1.
Potential impact of omega-3 treatment on cardiovascular disease in type 2 diabetes (2008)

This systematic review and meta-analysis confirms the triglyceride lowering effects of omega-3 PUFAs, demonstrates potential dose-response effects and shows improvements in thrombogenesis. Omega-3 PUFAs raise LDL levels without concomitant changes in lipid particle size. Changes seen in conventional risk factors are insufficient to explain the cardiovascular disease risk reductions suggested to occur with omega-3 PUFAs.
Glass capillary optics for making x-ray beams of 0.1 to 50 microns diameter (1997)

We have fabricated a unique computerized glass puller that can make parabolic or elliptically tapered glass capillaries for microbeam x-ray experiments from hollow glass tubing. We have produced optics that work in a single-bounce imaging mode or in a multi-bounce condensing mode. The imaging-mode capillaries have been used to create 20 to 50 micron diameter x-ray beams at 12 keV that are quite useful for imaging diffraction patterns from tiny bundles of carbon and Kevlar fibers. The condensing-mode capillaries are useful for creating submicron diameter beams and show great promise in x-ray fluorescence applications with femtogram sensitivity for patterned Er and Ti dopants diffused into an optically-active lithium niobate wafer.