gebhart/patrepeval huggingface dataset:
Rational Design of a Calcium Sensing System Based on Induced Conformational Changes of Calmodulin (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTRational Design of a Calcium Sensing System Based on Induced Conformational Changes of CalmodulinVesna Schauer-Vukasinovic, Lawrence Cullen, and Sylvia DaunertView Author Information Department of Chemistry, University of Kentucky Lexington, Kentucky 40506-0055 Cite this: J. Am. Chem. Soc. 1997, 119, 45, 11102–11103Publication Date (Web):November 12, 1997Publication History Received27 June 1997Published online12 November 1997Published inissue 1 November 1997https://pubs.acs.org/doi/10.1021/ja972139ohttps://doi.org/10.1021/ja972139orapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views260Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Fluorescence,Hydrophobicity,Labeling,Monomers,Peptides and proteins Get e-Alerts
Waveguiding mechanism in tube lattice fibers (2010)

Waveguiding mechanism and modal characteristics of hollow core fibers consisting of a single or a regular arrangement of dielectric tubes are investigated. These fibers have been recently proposed as low loss, broadband THz waveguides. By starting from a description in terms of coupling between air and dielectric modes in a single tube waveguide, a simple and useful model is proposed and numerically validated. It is able to predict dispersion curves, high and low loss spectral regions, and the conditions to ensure the existence of low loss regions. In addition, it allows a better understanding of the role of the geometrical parameters and of the dielectric refractive index. The model is then applied to improve the tradeoff between low loss and effectively single mode propagation, showing that the best results are obtained with a heptagonal arrangement of the tubes.
Hyperhomocysteinemia Confers an Independent Increased Risk of Atherosclerosis in End-Stage Renal Disease and Is Closely Linked to Plasma Folate and Pyridoxine Concentrations (1996)

A high total plasma homocysteine concentration is an independent risk factor for atherosclerotic complications of end-stage renal disease. Such patients may benefit from higher doses of B vitamins than those currently recommended.
Video Scene Parsing with Predictive Feature Learning (2016)

In this work, we address the challenging video scene parsing problem by developing effective representation learning methods given limited parsing annotations. In particular, we contribute two novel methods that constitute a unified parsing framework. (1) \textbf{Predictive feature learning}} from nearly unlimited unlabeled video data. Different from existing methods learning features from single frame parsing, we learn spatiotemporal discriminative features by enforcing a parsing network to predict future frames and their parsing maps (if available) given only historical frames. In this way, the network can effectively learn to capture video dynamics and temporal context, which are critical clues for video scene parsing, without requiring extra manual annotations. (2) \textbf{Prediction steering parsing}} architecture that effectively adapts the learned spatiotemporal features to scene parsing tasks and provides strong guidance for any off-the-shelf parsing model to achieve better video scene parsing performance. Extensive experiments over two challenging datasets, Cityscapes and Camvid, have demonstrated the effectiveness of our methods by showing significant improvement over well-established baselines.
Stability and preservative effectiveness of treprostinil sodium after dilution in common intravenous diluents (2003)

The stability of treprostinil sodium after dilution in three common i.v. infusion vehicles was assessed. The chemical stability of treprostinil sodium was tested over a 48-hour period at 40 degrees C and 75% relative humidity after dilution in each of three diluents: sterile water for injection, 0.9% sodium chloride injection, and 5% dextrose injection, and after passage through an i.v. delivery system. Chemical analysis was conducted by using a validated stability-indicating high-performance liquid chromatographic assay, visually inspecting the solutions, and measuring the pH of each solution. The preservative effectiveness of the solutions was tested by the recovery of inoculations of compendial microorganisms after 48 hours in dilute solutions of treprostinil sodium. All assay results for treprostinil were within 90.0% to 110.0% of the prepared solutions diluted at 0.004 and 0.13 mg/mL treprostinil sodium in sterile water for injection and 0.9% sodium chloride injection. The assay results were the same for dilute treprostinil solutions in 5% dextrose injection at concentrations of 0.02 and 0.13 mg/mL. The pH values for these solutions remained within acceptable values of 6.0 to 7.2 for the stability study. No change in physical appearance or any visible particulate matter was observed. Approximately 70% of metacresol, the preservative, in the dilute treprostinil sodium solutions was removed before reaching the terminal end of the tubing. None of the dilute treprostinil sodium solutions supported microbial growth in the cassette reservoirs for the organisms considered. Treprostinil sodium 0.13 mg/mL solution in sterile water for injection, 0.9% sodium chloride for injection, and 5% dextrose for injection appeared to be stable after storage in controlled ambulatory drug-delivery systems for 48 hours at 40 degrees C and 75% relative humidity. Treprostinil sodium 0.004 mg/mL in sterile water and 0.9% sodium chloride for injection and 0.02 mg/mL in 5% dextrose injection was also stable under the same conditions. None of the solutions showed signs of microbial growth.
Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G 1 /S checkpoint activation in response to DNA damage (2001)

ATR [ataxia-telangiectasia-mutated (ATM)- and Rad3-related] is a protein kinase required for both DNA damage-induced cell cycle checkpoint responses and the DNA replication checkpoint that prevents mitosis before the completion of DNA synthesis. Although ATM and ATR kinases share many substrates, the different phenotypes of ATM- and ATR-deficient mice indicate that these kinases are not functionally redundant. Here we demonstrate that ATR but not ATM phosphorylates the human Rad17 (hRad17) checkpoint protein on Ser(635) and Ser(645) in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of hRad17 in cells in early-mid G(1). Expression of kinase-inactive ATR resulted in reduced phosphorylation of these residues, but these same serine residues were phosphorylated in ionizing radiation (IR)-treated ATM-deficient human cell lines. IR-induced phosphorylation of hRad17 was also observed in ATM-deficient tissues, but induction of Ser(645) was not optimal. Expression of a hRad17 mutant, with both serine residues changed to alanine, abolished IR-induced activation of the G(1)/S checkpoint in MCF-7 cells. These results suggest ATR and hRad17 are essential components of a DNA damage response pathway in mammalian cells.
1.54-μm luminescence of erbium-implanted III-V semiconductors and silicon (1983)

Well-resolved sharply structured luminescence spectra at 1.54 μm were observed in erbium-implanted GaP, GaAs, InP, and Si. The optical transitions occur between the weakly crystal field split spin-orbit levels, 4I13/2→4I15/2, of Er3+(4f11). Typical spectral linewidths in GaAs are 2 cm−1(0.25 meV) at 6 K and 11 cm−1(1.36 meV) at room temperature.
A quantitative method for assessing stages of the rat estrous cycle (2005)

The impact of gender and/or hormone variations on a wide variety of neural functions makes the choice between studying males or females (or both) of a given species difficult. Although female rats are widely used experimentally, few studies control for the stage of estrus. More detailed information about how to distinguish the various stages of the estrous cycle is needed. For the present study, vaginal smears were obtained once a day and stained using an adaptation of the Papanicolaou (PAP) procedure. Images are provided of unstained "wet" samples and the corresponding PAP stained smears illustrating the cellular profile for each stage of the cycle as well as post-ovariectomy. The different cell populations across the cycle were quantified and ratios determined to show trends between the predominant and other cell types in each stage of the estrous cycle. Both stained and unstained images and cell quantification data provide valuable guidelines for distinguishing the stages of the estrous cycle.
Stereocontrolled Synthesis of (±)-12a-Deoxytetracycline (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTStereocontrolled Synthesis of (±)-12a-DeoxytetracyclineGilbert Stork, James J. La Clair, Peter Spargo, Ravi P. Nargund, and Nancy TotahView Author Information Department of Chemistry, Columbia University New York, New York 10027Cite this: J. Am. Chem. Soc. 1996, 118, 22, 5304–5305Publication Date (Web):June 5, 1996Publication History Received9 February 1996Published online5 June 1996Published inissue 5 June 1996https://pubs.acs.org/doi/10.1021/ja960434nhttps://doi.org/10.1021/ja960434nrapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views3569Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Adducts,Cyclization,Lactones,Organic compounds,Stereochemistry Get e-Alerts
Initial operating capability for the hypercluster parallel-processing test bed (1989)

The NASA Lewis Research Center is investigating the benefits of parallel processing to applications in computational fluid and structural mechanics. To aid this investigation, NASA Lewis is developing the Hypercluster, a multi-architecture, parallel-processing test bed. The initial operating capability (IOC) being developed for the Hypercluster is described. The IOC will provide a user with a programming/operating environment that is interactive, responsive, and easy to use. The IOC effort includes the development of the Hypercluster Operating System (HYCLOPS). HYCLOPS runs in conjunction with a vendor-supplied disk operating system on a Front-End Processor (FEP) to provide interactive, run-time operations such as program loading, execution, memory editing, and data retrieval. Run-time libraries, that augment the FEP FORTRAN libraries, are being developed to support parallel and vector processing on the Hypercluster. Special utilities are being provided to enable passage of information about application programs and their mapping to the operating system. Communications between the FEP and the Hypercluster are being handled by dedicated processors, each running a Message-Passing Kernel, (MPK). A shared-memory interface allows rapid data exchange between HYCLOPS and the communications processors. Input/output handlers are built into the HYCLOPS-MPK interface, eliminating the need for the user to supply separate I/O support programs on the FEP.
Conformational restriction of the phenylalanine residue in a cyclic opioid peptide analog: effects on receptor selectivity and stereospecificity (1991)

In an effort to determine the effect of side chain conformational restriction on opioid receptor selectivity, the cyclic phenylalanine analogues 2-aminoindan-2-carboxylic acid (Aic), 2-aminotetralin-2-carboxylic acid (Atc), and tetrahydroisoquinoline-3-carboxylic acid (Tic) were substituted for Phe in the potent cyclic opioid peptide analogue H-Tyr-D-Orn-Phe-Glu-NH2, which lacks significant opioid receptor selectivity. Compounds were tested in mu- and delta-opioid receptor representative binding assays and bioassays in vitro. The analogue H-Tyr-D-Orn-Aic-Glu-NH2 was found to be a potent agonist with high preference of mu receptors over delta receptors. Opening of the five-membered ring of Aic in the latter peptide, as achieved through substitution of C alpha-methylphenylalanine or o-methylphenylalanine, resulted in only slightly selective compounds, indicating that the high mu selectivity of the Aic analogue is exclusively the consequence of the imposed side chain conformational restriction. Both diastereoisomers of H-Tyr-D-Orn-(D,L)-Atc-Glu-NH2 were highly mu-selective and, in contrast to the weak affinity observed with the D-Phe3 analogue as compared to the L-Phe3 analogue, both had similar potency. Thus, stereospecificity was lost as a consequence of side chain conformational restriction. Further structure-activity data obtained with analogues containing L- or D-homophenylalanine (Hfe) or 3-(1'-naphthyl)alanine (Nap) in place of Phe3 and consideration of geometric interrelationships between Nap and the L and D isomers of Atc, Hfe, and Phe led to the proposal that the D-Phe3 and the D-Atc3 analogue may have different modes of binding to the receptor. The very low potency observed with H-Tyr-D-Orn-N alpha MePhe-Glu-NH2 (N alpha MePhe = N alpha-methylphenylalanine) and H-Tyr-D-Orn-Tic-Glu-NH2 indicated that N alpha-alkylation at the 3-position is detrimental to activity.
Bile Acids (1968)

Abstract 5α-Cholestan-3β-ol-4-14C (0.2 to 0.4 mg, 107 dpm) was administered intraperitoneally to rats made hyperthyroid by daily subcutaneous injections of l-thyroxine (250 µg per kg per day) for a period of 4 weeks prior to cannulation of the bile ducts. The bile secreted over 17 postoperative days contained 72% of the administered 14C. Bile from the first 2 days was hydrolyzed, and the free bile acids were separated by acetic acid partition chromatography. Of the chromatographed 14C, 38% was associated with dihydroxy acids, 48% with cholic acid, and 9% with more polar fractions. The metabolite in the first zone was separated from known acids by repeated chromatography of the methyl esters and identified by dilution with synthetic allochenodeoxycholic acid. The synthetic material was prepared from methyl chenodeoxycholate by conversion to methyl 3-keto-7α-hydroxy-5α-cholanoate with Raney nickel, followed by catalytic reduction, and its structure was supported by mass spectral studies of the acid and its derivatives.
Surface Coil Magnetic Resonance Imaging (1984)

A surface coil is used as a receiver in a crossed-coil arrangement with a conventional circumferential transmitter coil in a magnetic resonance imaging system. The improved signal-to-noise ratio for objects close to the coil, compared with a conventional circumferential saddle-shaped receiver coil, permits higher resolution imaging of relatively superficial structures such as the orbit, neck, chest wall, and lumbar spine.
Honey bee colony collapse and changes in viral prevalence associated with Varroa destructor (2010)

"Honey bee colony collapse and changes in viral prevalence associated with Varroa destructor." Journal of Apicultural Research, 49(1), pp. 93–94Keywords: Varroa destructorApis melliferadeformed wing virusslow paralysis viruscloudy wing virus
Transforming growth factor beta production during rat cytomegalovirus infection. (1997)

We analysed the production of transforming growth factor beta (TGF-beta) during a cytomegalovirus (CMV) infection in a rat model system. Splenocytes from immunocompetent rats infected with rat CMV (RCMV) released increased amounts of TGF-beta1. TGF-beta production was also evident in RCMV-infected radiation-immunosuppressed rats; their sera inhibited the interleukin 2-induced proliferation of T cells, which could be restored by anti-TGF-beta antibodies. In addition, TGF-beta production could be visualized immunohistologically in the lungs, spleen, liver and bone marrow of radiation-immunosuppressed infected rats. The virus directly induced this cytokine since TGF-beta was produced upon RCMV infection in vitro. The induction of TGF-beta production may contribute to immunosuppression during CMV infection.
Multimedia retrieval through spatio-temporal activity maps (2001)

As multiple video cameras and other sensors generate very large quantities of multimedia data in media productions and surveillance applications, a key challenge is to identify the relevant portions of the data and to rapidly retrieve the corresponding sensor data. Spatio-temporal activity maps serve as an efficient and intuitive graphical user interface for multimedia retrieval, particularly when the media streams are derived from multiple sensors observing a physical environment. We formulate the media retrieval problem in this context, and develop an architecture for interactive media retrieval by combining spatio-temporal "activity maps" with domain specific event information. Activity maps are computed from trajectories of motion of objects in the environment, which in turn are derived automatically by analysis of sensor data. We present an activity map based video retrieval system for the sport of tennis and demonstrate that the activity map based scheme significantly helps the user in a) discovering the relevant portions of the data, and b) non-linearly retrieving the corresponding media streams.
Transcription of pfl is regulated by anaerobiosis, catabolite repression, pyruvate, and oxrA: pfl::Mu dA operon fusions of Salmonella typhimurium (1989)

Pyruvate formate-lyase (EC 2.3.1.54), a key enzyme in the anaerobic metabolism of Salmonella typhimurium, catalyzes the conversion of pyruvate to acetyl coenzyme A and formate. pfl::Mu dA operon fusions were isolated for the study of transcriptional regulation. pfl was transcribed both aerobically and anaerobically, but the activity increased about sixfold under anaerobic conditions. The addition of pyruvate, formate, and acetate in nutrient broth did not have any effect on the anaerobic expression of pfl. However, the addition of pyruvate to minimal glucose medium increased the anaerobic expression of pfl. The expression of pfl varied in different growth media. Anaerobic expression of pfl was lower when the culture was grown in minimal glucose medium than when it was grown in nutrient broth. When Casamino Acids (Difco Laboratories, Detroit, Mich.) were added to minimal glucose medium, the expression of pfl increased proportionally with the amount of Casamino Acids added. The transcription of pfl was positively controlled by the oxrA gene product and was affected by both the cya and crp mutations. However, mutations in genes affecting the cyclic AMP-cyclic AMP receptor protein complex or oxrA could not completely abolish the anaerobic derepression of pfl. In merodiploid strains, pfl::Mu dA/F' pfl+, the beta-galactosidase activities were decreased. The mutations gyrA, oxrC, and oxrE, which affected anaerobic metabolism, did not affect anaerobic expression of pfl.
Asialoglycoprotein Receptor (ASGP-R) is Liver Parenchymal Cells is Involved in Elimination of Recombinant Human TFPI (2010)

We here report on a study carried out to determine the early clearance kinetics, and organ, cell(s) and receptor(s) responsible for the clearance of full length TFPI purified from BHK cells (TFPI&lt;sup&gt;BHK&lt;/sup&gt;). Following intravenous administration, &lt;sup&gt;125&lt;/sup&gt;I-TFPI&lt;sup&gt;BHK&lt;/sup&gt; was cleared with a\nbiphasic elimination curve, and with a significantly slower t&lt;sub&gt;1/2&lt;/sub&gt;α compared to recombinant human TFPI from &lt;i&gt;E.Coli&lt;/i&gt; (TFPI&lt;sup&gt;E.Coli&lt;/sup&gt;) (1.95±0.10 versus 1.42±0.07 min, respectively, p&lt;0.001). Studies on organ and cell distribution revealed that liver parenchymal cells (PCs) were responsible for 96% of the uptake of TFPI&lt;sup&gt;BHK&lt;/sup&gt; and 81% of TFPI&lt;sup&gt;E.Coli&lt;/sup&gt;, whereas the nonparenchymal cells (NPCs) were responsible for 4% and 19%, respectively. Pre-administration of excessive amounts of unlabeled TFPI&lt;sup&gt;BHK&lt;/sup&gt; prolonged blood clearance of &lt;sup&gt;125&lt;/sup&gt;I-TFPI&lt;sup&gt;BHK&lt;/sup&gt;. Unlabelled TFPI&lt;sup&gt;BHK&lt;/sup&gt; inhibited endocytosis of &lt;sup&gt;125&lt;/sup&gt;I-TFPI&lt;sup&gt;BHK&lt;/sup&gt; in PCs &lt;i&gt;in vitro&lt;/i&gt;, whereas blocking\nof LDL-receptor related protein-1 (LRP-1) by receptor-associated protein (RAP) affected\nneither blood clearance nor endocytosis of &lt;sup&gt;125&lt;/sup&gt;I-TFPI&lt;sup&gt;BHK&lt;/sup&gt; in PCs. In addition, TFPI&lt;sup&gt;BHK&lt;/sup&gt; was also\nfound in the kidneys and this could be reduced in the presence of RAP. Asialoorosomucoid (ASOR), a potent inhibitor of the asialoglycoprotein receptor (ASGP-R), prolonged the circulatory survival of &lt;sup&gt;125&lt;/sup&gt;I-m-TFPI by 1.5-fold (p&lt;0.001). &lt;i&gt;In vitro&lt;/i&gt;, ASOR and other ASGP-R antagonists significantly inhibited endocytosis of &lt;sup&gt;125&lt;/sup&gt;I-TFPI&lt;sup&gt;BHK&lt;/sup&gt; in PCs. Moreover, unlabelled TFPI&lt;sup&gt;BHK&lt;/sup&gt; markedly decreased endocytosis of &lt;sup&gt;125&lt;/sup&gt;I-asialofetuin. In conclusion, our findings\nsuggest that ASGP-R mediated endocytosis in the liver is involved in the clearance of\nTFPI&lt;sup&gt;BHK&lt;/sup&gt;.