gebhart/patrepeval huggingface dataset:
α-Synucleinopathy and selective dopaminergic neuron loss in a rat lentiviral-based model of Parkinson's disease (2002)

Parkinson's disease (PD) is characterized by the progressive loss of substantia nigra dopaminergic neurons and the presence of cytoplasmic inclusions named Lewy bodies. Two missense mutations of the alpha-synuclein (alpha-syn; A30P and A53T) have been described in several families with an autosomal dominant form of PD. alpha-Syn also constitutes one of the main components of Lewy bodies in sporadic cases of PD. To develop an animal model of PD, lentiviral vectors expressing different human or rat forms of alpha-syn were injected into the substantia nigra of rats. In contrast to transgenic mice models, a selective loss of nigral dopaminergic neurons associated with a dopaminergic denervation of the striatum was observed in animals expressing either wild-type or mutant forms of human alpha-syn. This neuronal degeneration correlates with the appearance of abundant alpha-syn-positive inclusions and extensive neuritic pathology detected with both alpha-syn and silver staining. Lentiviral-mediated expression of wild-type or mutated forms of human alpha-syn recapitulates the essential neuropathological features of PD. Rat alpha-syn similarly leads to protein aggregation but without cell loss, suggesting that inclusions are not the primary cause of cell degeneration in PD. Viral-mediated genetic models may contribute to elucidate the mechanism of alpha-syn-induced cell death and allow the screening of candidate therapeutic molecules.
Expression of EDA/EDB isoforms of fibronectin in papillary carcinoma of the thyroid (1999)

Cellular fibronectins containing the extracellular domain A or B (EDA and EDB) are particularly abundant in fetal and neoplastic tissues. The presence of EDA and EDB was investigated in 28 cases of papillary carcinoma of the thyroid using IST-9 and BC-1 monoclonal antibodies. Immunostaining for EDA and EDB was detected in tumour stroma, in tumour basement membranes, and in tumour blood vessels. EDA was present in 27 of the 28 cases, in 20 of which more than 75 per cent of the tumour stroma was stained. Immunostaining for EDB was detected in 23 of the 28 cases and was less pronounced than that for EDA, being present in less than 25 per cent of the tumour stroma in most cases. Reactivity for EDA/EDB was not observed in the adjacent normal thyroid in any of the cases investigated. In a group of 20 non-papillary tumours, immunostaining for EDA was present in the stroma of three follicular carcinomas (one minimally and two widely invasive), one medullary carcinoma, and 5 of 16 follicular adenomas; expression of EDB was more restricted, being present in only the two cases of widely invasive follicular carcinoma. The presence of EDA and EDB was not correlated with the extent of fibrosis or the degree of tumour cell differentiation. Immunoreactivity was already present in microcarcinomas. These observations raise the possibility that the production of oncofetal fibronectins is an important step in papillary carcinoma tumourigenesis, perhaps facilitating adhesion and spreading of tumour cells.
ZFHX4 Interacts with the NuRD Core Member CHD4 and Regulates the Glioblastoma Tumor-Initiating Cell State (2014)

Glioblastoma (GBM) harbors subpopulations of therapy-resistant tumor-initiating cells (TICs) that are self-renewing and multipotent. To understand the regulation of the TIC state, we performed an image-based screen for genes regulating GBM TIC maintenance and identified ZFHX4, a 397 kDa transcription factor. ZFHX4 is required to maintain TIC-associated and normal human neural precursor cell phenotypes in vitro, suggesting that ZFHX4 regulates differentiation, and its suppression increases glioma-free survival in intracranial xenografts. ZFHX4 interacts with CHD4, a core member of the nucleosome remodeling and deacetylase (NuRD) complex. ZFHX4 and CHD4 bind to overlapping sets of genomic loci and control similar gene expression programs. Using expression data derived from GBM patients, we found that ZFHX4 significantly affects CHD4-mediated gene expression perturbations, which defines ZFHX4 as a master regulator of CHD4. These observations define ZFHX4 as a regulatory factor that links the chromatin-remodeling NuRD complex and the GBM TIC state.
Iron metabolism disturbances in the MCF-7 human breast cancer cells with acquired resistance to doxorubicin and cisplatin (2013)

The development of resistance of cancer cells to therapeutic agents is the major obstacle in the successful treatment of breast cancer and the main cause of breast cancer recurrence. The results of several studies have demonstrated an important role of altered cellular iron metabolism in the progression of breast cancer and suggested that iron metabolism may be involved in the acquisition of a cancer cell drug-resistant phenotype. In the present study, we show that human MCF-7 breast cancer cells with an acquired resistance to the chemotherapeutic drugs doxorubicin (MCF-7/DOX) and cisplatin (MCF-7/CDDP) exhibited substantial alterations in the intracellular iron content and levels of iron-regulatory proteins involved in the cellular uptake, storage and export of iron, especially in profoundly increased levels of ferritin light chain (FTL) protein. The increased levels of FTL in breast cancer indicate that FTL may be used as a diagnostic and prognostic marker for breast cancer. Additionally, we demonstrate that targeted downregulation of FTL protein by the microRNA miR-133a increases sensitivity of MCF-7/DOX and MCF-7/CDDP cells to doxorubicin and cisplatin. These results suggest that correction of iron metabolism abnormalities may substantially improve the efficiency of breast cancer treatment.
In vitro translation of the full-length RNA transcript of figwort mosaic virus (Caulimovirus). (1996)

The circular DNA genome of FMV consists of seven tandemly arranged genes placed successively on a full-length RNA transcript that spans the entire circular viral genome. This transcript is a tentative mRNA for at least five of the six major conserved genes of this virus (genes I-V) that are positioned on this transcript. The sixth major gene (gene VI) is expressed as a separate monocistronic transcript. A long 5'-nontranslated leader (598 nucleotides), a small nonconserved gene (VII), and a short intergenic region (57 nucleotides) precede the five major conserved genes (I through V) on the full-length transcript. A reporter gene (CAT), as a separate cistron or fused in-frame, to viral cistrons in various downstream positions in cloned versions of the viral genome was used in a transcription vector to generate artificial full-length transcripts of FMV. When these mRNAs were translated in vitro (rabbit reticulocyte lysate system), the reporter gene was translated efficiently in all positions. Translation of internal native viral gene positioned on the full-length transcript of FMV was also determined (the gene VI product). These observations suggest that the full-length FMV transcript functions as a polycistronic mRNA in plants. Results are best explained on the basis of translational coupling/relay race model.
Surgical therapy for Parkinson's disease (2002)

Surgical therapies for Parkinson's disease (PD) are now being performed with increasing frequency due to the limitations of conventional dopaminergic therapies, improvements in operative procedures, and increased information on the organization of the basal ganglia in normal and pathologic conditions. Ablation procedures have now been largely replaced with deep brain stimulation, which permits benefits to be obtained without the need to make a destructive brain lesion. Several studies now demonstrate the value of stimulating the subthalamic nucleus or the globus pallidus pars interna in patients with advanced PD. Nonetheless, there are limitations associated with these procedures and benefits do not exceed those obtained with levodopa, albeit with reduced motor complications. Fetal transplantation remains an experimental procedure that has shown limited benefits in a double-blind trial and is complicated by persistent dyskinesia. Stem cell, trophic factor, and gene therapy approaches are promising and are currently under intensive investigation.
Silicon MOSFET-based field-induced band-to-band tunneling eftect transistor - "FIBTET" (2004)

In this paper, a novel FIBTETs with degenerate channel doping concentration in the simple SOI MOSFET structure were fabricated and their reproducible NDT (negative-differential transconductance) characteristics was observed at room temperature both for n-FIBTETs and p-FIBTETs. The size dependence of the device characteristics showed a good controllability and reproducibility, which is advantageous for the mass production of silicon-based tunneling devices in the IC industry.
Noisy silence (2013)

A significant fraction of eukaryotic genomes comprises repetitive sequences, including rRNA genes, centromeres, telomeres, and retrotransposons. Repetitive elements are hotspots for recombination and represent a serious challenge for genome integrity. Maintaining these repeated elements in a compact heterochromatic structure suppresses recombination and unwanted mutagenic transposition, and is therefore indispensable for genomic stability. Paradoxically, repetitive elements are not transcriptionally inert, but produce RNA that has important functions in regulating and reinforcing the heterochromatic state. Here, we review the role of non-coding RNA (ncRNA) in recruiting chromatin-modifying enzymes to repetitive genomic loci to establish a repressive chromatin structure that safeguards chromosome integrity and genome stability.
Intranasal dexmedetomidine vs midazolam for premedication in children undergoing complete dental rehabilitation: a double‐blinded randomized controlled trial (2013)

Intranasal dexmedetomidine (1 μg·kg(-1)) is an effective and safe alternative for premedication in children; it resulted in superior sedation in comparison to 0.2 mg·kg(-1) intranasal midazolam. However, it has relatively prolonged onset of action.
Metric projector camera calibration for measurement applications (2006)

Structured light is widely used for shape measurement of surfaces using the triangulation principle. To fulfill this task, precise information about the optical path is necessary. We propose a novel method for the overall calibration of a setup consisting of a structured light source, a projection screen and a camera. As computer controlled video projectors become cheaper and cheaper, it is reasonable to use these off-the-shelf devices for measurement applications. However, to achieve high accuracy with standard components, a precise calibration of the measurement system is indispensable. Absolute position and orientation of the camera, the projector and the projection screen has to be known. Furthermore, intrinsic calibration of both the camera and the projector is necessary. After acquiring a large set of data points using a versatile phase encoding technique, we estimate the optimal parameters using a bundle adjustment technique. We consider all extrinsic and intrinsic parameters for the optical mapping including a distortion model for the projector and for the camera, respectively. We propose a method which ensures complete knowledge about the optical mapping of each ray observed by the camera. The proposed metric calibration method has also importance for other measurement applications as e.g. shape reconstruction of specular surfaces. Hereby structured light patterns are projected on the screen, and their reflection on the specular surface is observed by the camera.
Recognition of Simple Gestures Using a PIR Sensor Array (2012)

We present an approach that is intended for simple gesture control using a relatively inexpensive pyroelectric array detector. The detector is manufactured using standard wafer processing techniques. It consists of a 16 element passive infrared sensor array that responds to changing infrared signals, such as are generated by a hand moving at a distance of some tens of centimetres in front of the array. There is quite a large variation in the responsivity of the pixels within the array, but despite that it is relatively easy to use differential signals from the array or to apply a simplified version of an image processing algorithm to track movement in front of the detector. We have developed a prototype system that can recognise hand movements in different directions in front of the detector. This has allowed us to develop a demonstrator system that can be used to control, for instance, a PowerPoint presentation by gesture.
IMGT-Choreography for immunogenetics and immunoinformatics. (2005)

IMGT, the international ImMunoGeneTics information system (http://imgt.cines.fr), was created in 1989 at Montpellier, France. IMGT is a high quality integrated knowledge resource specialized in immunoglobulins (IG), T cell receptors (TR), major histocompatibility complex (MHC) of human and other vertebrates, and related proteins of the immune system (RPI) which belong to the immunoglobulin superfamily (IgSF) and MHC superfamily (MhcSF). IMGT provides a common access to standardized data from genome, proteome, genetics and three-dimensional structures. The accuracy and the consistency of IMGT data are based on IMGT-ONTOLOGY, a semantic specification of terms to be used in immunogenetics and immunoinformatics. IMGT-ONTOLOGY has been formalized using XML Schema (IMGT-ML) for interoperability with other information systems. We are developing Web services to automatically query IMGT databases and tools. This is the first step towards IMGT-Choreography which will trigger and coordinate dynamic interactions between IMGT Web services to process complex significant biological and clinical requests. IMGT-Choreography will further increase the IMGT leadership in immunogenetics and immunoinformatics for medical research (repertoire analysis of the IG antibody sites and of the TR recognition sites in autoimmune and infectious diseases, AIDS, leukemias, lymphomas, myelomas), veterinary research (IG and TR repertoires in farm and wild life species), genome diversity and genome evolution studies of the adaptive immune responses, biotechnology related to antibody engineering (single chain Fragment variable (scFv), phage displays, combinatorial libraries, chimeric, humanized and human antibodies), diagnostics (detection and follow up of residual diseases) and therapeutical approaches (grafts, immunotherapy, vaccinology). IMGT is freely available at http://imgt.cines.fr.
Imitating human dance motions through motion structure analysis (2003)

This paper presents a method for importing human dance motion into humanoid robots through visual observation. The human motion data is acquired from a motion capture system consisting of 8 cameras and 8 PC clusters. Then the whole motion sequence is divided into motion elements and clustered into groups according to the correlation of end-effector trajectories. We call these segments 'motion primitives'. New dance motions are generated by concatenating these motion primitives. We are also trying to make a humanoid dance these original or generated motions using inverse-kinematics and dynamic balancing techniques.
Manipulation of confined bubbles in a thin microchannel: Drag and acoustic Bjerknes forces (2011)

Bubbles confined between the parallel walls of microchannels experience an increased drag compared to freestanding bubbles. We measure and model the additional friction from the walls, which allows the calibration of the drag force as a function of velocity. We then develop a setup to apply locally acoustic waves and demonstrate the use of acoustic forces to induce the motion of bubbles. Because of the bubble pulsation, the acoustic forces—called Bjerknes forces—are much higher than for rigid particles. We evaluate these forces from the measurement of bubble drift velocity and obtain large values of several hundreds of nanonewtons. Two applications have been developed to explore the potential of these forces: asymmetric bubble breakup to produce very well controlled bidisperse populations and intelligent switching at a bifurcation.
Mirroring the ADS Bibliographic Databases (1998)

. During the past year the Astrophysics Data System has set up two mirror sites for the benefit of users around the world with a slow network connection to the main ADS server in Cambridge, MA. In order to clone the ADS abstract and article services on the mirror sites, the structure of the bibliographic databases, query forms and search scripts has been made both site- and platform-independent by creating a set of configuration parameters that define the characteristics of each mirror site and by modifying the database management software to use such parameters. Regular updates to the databases are performed on the main ADS server and then mirrored on the remote sites using a modular set of scripts capable of performing both incremental and full updates. The use of software packages capable of authentication, as well as data compression and encryption, permits secure and fast data transfers over the network, making it possible to run the mirroring procedures in an unsupervised fashio...
The Porcine Ig δ Gene: Unique Chimeric Splicing of the First Constant Region Domain in its Heavy Chain Transcripts (2003)

The pig delta gene is located approximately 3.4 kb downstream of the second transmembrane exon of the micro gene and shows a similar genomic structure to its counterpart in cow with three exons encoding the CH1, CH2, and CH3 domains. The porcine genomic deltaCH1 exon has been replaced by a recent duplication of the micro CH1 and its flanking sequences, a genetic event that also led to the formation of a short switch delta region, immediately upstream of the delta gene. The deltaCH1 exhibits a 98.7% similarity (314 of 318 bp) to the micro CH1 at the DNA level, whereas the homologies between the deltaCH2 and micro CH3, and the deltaCH3 and micro CH4 are only 33.3 and 35.8%, respectively. Either of the two CH1 exons ( micro and delta) could be observed in the expressed porcine IgD H chain cDNA sequences VDJ- micro CH1-H-deltaCH2-deltaCH3 or VDJ-deltaCH1-H-deltaCH2-deltaCH3, showing a pattern that has not been observed previously in vertebrates. In addition, transfection of a human B cell line, using artificial constructs resembling the porcine C micro -Cdelta locus, also generated both VDJ- micro CH1-deltaCH1-H1-deltaCH2 and VDJ -deltaCH1-H1-deltaCH2 transcripts. An examination of the pig delta genomic sequence shows a putative, second hinge region-encoding exon. Due to the lack of a normal branchpoint sequence for RNA splicing, this exon is not present in the normal pig delta cDNA. However, the exon could be spliced into most of the expressed transcripts in vitro in cell transfection experiments after introduction of a single T nucleotide to restore the branchpoint sequence upstream of the putative H2 exon.
A New Lens For Submicron Lithography And Its Consequences For Wafer Stepper Design (1986)

The imaging properties of a prototype from the new generation of i-line, high numerical aperture projection lenses have been investigated. The production resolution of this submicron lens is 0.7 micron at a depth of focus of +/- 1.5 microns. The diameter of the image field is 14.1 mm. The curvature of the focal plane is about 1.5 microns between a position at the optical axis and a position at the edge of the image field. The lens has been incorporated in a wafer stepper. Improvements which have been carried out in order to meet the submicron requirements from the side of the stepper are the accuracy of the wafer positioning (3 sigma = 50 nm) and of reticle-to-wafer alignment ( 3 sigma = 60 nm ). Systems for correcting for the influence of variations of the atmospheric pressure have been incorporated.
Salicylate Regulates COX‐2 Expression Through ERK and Subsequent NF‐κB Activation in Osteoblasts (2004)

The expression of cyclooxygenase-2 (COX-2) is a characteristic response to inflammation and can be inhibited with sodium salicylate. TNF-alpha plus IFN-gamma can induce extracellular signal-regulated kinase (ERK), IKK, IkappaB degradation and NF-kappaB activation. The inhibition of the ERK pathway with selective inhibitor, PD098059, blocked cytokine-induced COX-2 expression and PGE2 release. Salicylate treatment inhibited COX-2 expression induced by TNF-alpha/IFN-gamma and regulated the activation of ERK, IKK and IkappaB degradation and subsequent NF-kappaB activation in MC3T3E1 osteoblasts. As well, antioxidant-catalase, N-acetyl-cysteine or reduced glutathione-attenuated COX-2 expression in combined cytokines-treated cells. These antioxidants also inhibited the activation of ERK, IKK and NF-kappaB in MC3T3E1 osteoblasts. In addition, TNF-alpha/IFN-gamma stimulated ROS release in the osteoblasts. However salicylate had no obvious effect on ROS release in DCFDA assay. The results showed that salicylate inhibited the activation of ERK and IKK, IkappaB degradation and NF-kappaB activation independent of ROS release and suggested that salicylate exerts its anti-inflammatory action in part through inhibition of the ERK, IKK, IkappaB, NF-kappaB and resultant COX-2 expression pathway.
Lewis acid mediated nucleophilic substitution reactions of 2-alkoxy-3,4-dihydro-2H-1-benzopyrans: regiochemistry and utility in the synthesis of 3,4-dihydro-2H-1-benzopyran-2-carboxylic acids (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLewis acid mediated nucleophilic substitution reactions of 2-alkoxy-3,4-dihydro-2H-1-benzopyrans: regiochemistry and utility in the synthesis of 3,4-dihydro-2H-1-benzopyran-2-carboxylic acidsNoal Cohen, Beatrice Schaer, Gabriel Saucy, Rene Borer, Louis Todaro, and Anne Marie ChiuCite this: J. Org. Chem. 1989, 54, 14, 3282–3292Publication Date (Print):July 1, 1989Publication History Published online1 May 2002Published inissue 1 July 1989https://pubs.acs.org/doi/10.1021/jo00275a010https://doi.org/10.1021/jo00275a010research-articleACS PublicationsRequest reuse permissionsArticle Views1176Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts