gebhart/patrepeval huggingface dataset:
Effect of off-axis illumination on the printability of opaque and transparent reticle defects (1995)

The effect of transparent and opaque reticle defects is examined with four illumination schemes used to enhance stepper resolution: standard with sigma 0.6, quadrapole, annular, and high coherence, sigma 0.3. It is found that transparent defects have greater printability than opaque defects. This difference in printability is more significant when the defect causes more than a 20% CD loss. In addition, defects centered between geometry defects have greater printability than opaque defects, and quadrapole illumination enhances the printability of defects compared to standard illumination. Process window analysis is used to shot that if both a reticle defect and a reticle CD error occur at the same point, the CD error effects add or subtract depending on their polarity. If +/- 10% CD control is required on 100% of the final gates of a circuit, one can not assign all 10% just to reticle defects. It is necessary to allow greater than +/- 10% variation on the wafer, or to allow less than 10% variation from reticle defects.
Lithography‐Free, Self‐Aligned Inkjet Printing with Sub‐Hundred‐Nanometer Resolution (2005)

Direct inkjet printing of sub-100 nm nanoscale gaps, without the need for any high-resolution lithography, is made possible by a novel self-aligned printing method (see Figure). The benefits of this method are demonstrated by fabrication of all-polymer transistor circuits, which exhibit switching speeds at least two orders of magnitude higher than those previously reported for inkjet-printed polymer devices.
Novel Preclinical and Radiopharmaceutical Aspects of [68Ga]Ga-PSMA-HBED-CC: A New PET Tracer for Imaging of Prostate Cancer (2014)

The detection of prostate cancer lesions by PET imaging of the prostate-specific membrane antigen (PSMA) has gained highest clinical impact during the last years. 68Ga-labelled Glu-urea-Lys(Ahx)-HBED-CC ([68Ga]Ga-PSMA-HBED-CC) represents a successful novel PSMA inhibitor radiotracer which has recently demonstrated its suitability in individual first-in-man studies. The radiometal chelator HBED-CC used in this molecule represents a rather rarely used acyclic complexing agent with chemical characteristics favourably influencing the biological functionality of the PSMA inhibitor. The simple replacement of HBED-CC by the prominent radiometal chelator DOTA was shown to dramatically reduce the in vivo imaging quality of the respective 68Ga-labelled PSMA-targeted tracer proving that HBED-CC contributes intrinsically to the PSMA binding of the Glu-urea-Lys(Ahx) pharmacophore. Owing to the obvious growing clinical impact, this work aims to reflect the properties of HBED-CC as acyclic radiometal chelator and presents novel preclinical data and relevant aspects of the radiopharmaceutical production process of [68Ga]Ga-PSMA-HBED-CC.
Autonomous Vehicle Control System Using an Image Processing Sensor (1995)

&lt;div class="htmlview paragraph"&gt;Development of a system which utilizes technology for the recognition of a vehicle's surroundings. Research is currently taking place in many countries, the goals of which are to improve a vehicle's safety and convenience by reducing driver strain through the automation of the recognition, judgment, and operation capabilities that are needed when driving an automobile.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;To recognize the vehicle's surroundings, we have adopted an image sensor system which offers superior three-dimensional recognition capabilities. In this paper, we will introduce a inter vehicle distance control system which controls the vehicle's throttle and braking functions by detecting the position of the driving lane and the distance to the vehicle in front based on the images obtained by a CCD camera.&lt;/div&gt;
Nitric oxide modulates oxygen consumption by arteriolar walls in rat skeletal muscle (2005)

To study the role of nitric oxide (NO) in regulating oxygen consumption by vessel walls, the oxygen consumption rate of arteriolar walls in rat cremaster muscle was measured in vivo during flow-induced vasodilation and after inhibiting NO synthesis. The oxygen consumption rate of arteriolar walls was calculated based on the intra- and perivascular PO2 values measured by phosphorescence quenching laser microscopy. The perivascular PO2 value of the arterioles during vasodilation was significantly higher than under control conditions, although the intravascular PO2 values under both conditions were approximately the same. Inhibition of NO synthesis, on the other hand, caused a significant increase in arterial blood pressure and a significant decrease in arteriolar diameter. Inhibition of NO synthesis also caused a significant decrease in both the intra- and perivascular PO2 values of the arterioles. Inhibition of NO synthesis increased the oxygen consumption rate of the vessel walls by 42%, whereas enhancement of flow-induced NO release decreased it by 34%. These results suggest that NO plays an important role not only as a regulator of peripheral vascular tone but also as a modulator of tissue oxygenation by reducing oxygen consumption by vessel walls. In addition, enhancement of NO release during exercise may facilitate efficient oxygen supply to the surrounding high metabolic tissue.
Concentration Dependent Cu2+Induced Aggregation and Dityrosine Formation of the Alzheimer's Disease Amyloid-β Peptide (2007)

The Amyloid beta peptide (Abeta) of Alzheimer's diseases (AD) is closely linked to the progressive cognitive decline associated with the disease. Cu2+ ions can induce the de novo aggregation of the Abeta peptide into non-amyloidogenic aggregates and the production of a toxic species. The mechanism by which Cu2+ mediates the change from amyloid material toward Cu2+ induced aggregates is poorly defined. Here we demonstrate that the aggregation state of Abeta1-42 at neutral pH is governed by the Cu2+:peptide molar ratio. By probing amyloid content and total aggregation, we observed a distinct Cu2+ switching effect centered at equimolar Cu2+:peptide ratios. At sub-equimolar Cu2+:peptide molar ratios, Abeta1-42 forms thioflavin-T reactive amyloid; conversely, at supra-equimolar Cu2+:peptide molar ratios, Abeta1-42 forms both small spherical oligomers approximately 10-20 nm in size and large amorphous aggregates. We demonstrate that these insoluble aggregates form spontaneously via a soluble species without the presence of an observable lag phase. In seeding experiments, the Cu2+ induced aggregates were unable to influence fibril formation or convert into fibrillar material. Aged Cu2+ induced aggregates are toxic when compared to Abeta1-42 aged in the absence of Cu2+. Importantly, the formation of dityrosine crosslinked Abeta, by the oxidative modification of the peptide, only occurs at equimolar molar ratios and above. The formation of dityrosine adducts occurs following the initiation of aggregation and hence does not drive the formation of the Cu2+ induced aggregates. These results define the role Cu2+ plays in modulating the aggregation state and toxicity of Abeta1-42.
Population genetic analysis of Borrelia burgdorferi isolates by multilocus enzyme electrophoresis (1992)

Fifty Borellia burgdorferi strains isolated from humans and ticks in Europe and the United States were analyzed by multilocus enzyme electrophoresis. Eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. Ten loci were polymorphic. The average number of alleles per locus was 5.9, with a mean genetic diversity of 0.673 among electrophoretic types (ETs). The strains were grouped into 35 ETs constituting three main divisions (I, II, and III) separated at a genetic distance greater than 0.75. Divisions I, II, and III contained 13, 6, and 16 ETs, respectively. These findings, together with previous data from DNA hybridization and restriction enzyme analysis of rRNA genes, suggest that divisions I, II, and III may represent three distinct genomic species. All three divisions contained human clinical ETs. However, in division I, which includes the ET of the type strain of B. burgdorferi, the human pathogenic ETs constituted a single clone. The ETs of division I were from west-central Europe and the United States, whereas divisions II and III contained ETs from west-central and northern Europe but not from the United States. Finally, our data show that the genetic structure of B. burgdorferi populations is clonal.
A new method of high resolution, quantitative phase scanning microscopy (2010)

Coherent Diffractive Imaging (CDI) is a method of lensless imaging that reconstructs a target object from recordings of the diffraction pattern it generates when illuminated by a coherent source. A new method of scanning CDI, 'Ptychography,' was introduced recently and has been successfully demonstrated as a method of lensless microscopy at optical and x-ray wavelengths. Here we show how it can be applied to visible light microscopy to produce high resolution quantitative phase images of low-contrast objects, such as unstained cells.
Percutaneous and Transdermal Oestrogen Replacement Therapy (1993)

The benefits of oestrogen replacement therapy (ERT) have been widely recognized. However, among the drawbacks ascribed to ERT, metabolic disturbances have been observed, particularly after oral administration of oestrogens. Therefore, other routes of delivering oestrogens have been developed. The advantages of delivering drugs through the skin for systemic therapy have been widely recognized and represent a growing sector in drug development. Transdermal delivery of steroids is also a rapidly expanding field and in various clinical situations where hormonal replacement therapy is needed this route of administration is a real breakthrough, considering the relative toxicity of some steroids when given orally. Percutaneous application of steroids has been proven successful in various disorders. Various occlusive transdermal systems have also been designed, all of them aimed at achieving a constant release rate of the molecules contained in their reservoir through the intact skin. The skin itself, and especially the outermost layer, the stratum corneum, can play the role of a reservoir and a rate-controlling membrane. In the field of reproductive hormones, so far, oestradiol, progesterone and testosterone have been demonstrated to be good candidates for transdermal delivery. The effectiveness and the acceptability of transdermal delivery of oestradiol in postmenopausal women have been demonstrated. All the beneficial effects of oestrogen therapy can be achieved through skin delivery, e.g. symptoms relief as well as osteoporosis prevention.
On the Interaction between a Fully lonized Plasma and a Neutral Gas Blanket (1970)

Experimental studies have been performed of a fully ionized magnetized plasma generated in a poloidal magnetic field by a transverse electric field applied between two electrodes and being surrounded by a neutral gas blanket: (i)The rotating plasma which has been generated in the crossed fields is released from its energy source and its rotation is being stopped. The plasma decay is then studied in the absence of macroscopic motions, with the exception of those from a slow diffusion process. The state of ionization is determined by suddenly giving the plasma a small pulse of angular momentum which, after a short time is recovered as a current pulse being extracted by means of a short-circuit between the electodes. The pulse becomes a measure of the state of ionization and shows that the rate of penetration of neutral gas into the plasma is strongly delayed at high plasma densities, in agreement with theory. (ii)The velocity field of a quasi-stationary, rotating plasma has been investigated spectroscopically by observing the Doppler shifts of various spectral lines. The results agree with theoretical perdictions of the velocity and temperature distributions within the plasma region. The results also support the prediction that the interior of the plasma should be screened from the surrounding neutral gas.
c-mpl mutations are the cause of congenital amegakaryocytic thrombocytopenia (2001)

Congenital amegakaryocytic thrombocytopenia (CAMT) is a rare disease presenting with isolated thrombocytopenia in infancy and developing into a pancytopenia in later childhood. Thrombopoietin (TPO) is the main regulator of thrombocytopoiesis and has also been demonstrated to be an important factor in early hematopoiesis. We analyzed 9 patients with CAMT for defects in TPO production and reactivity. We found high levels of TPO in the sera of all patients. However, platelets and hematopoietic progenitor cells of patients with CAMT did not show any reactivity to TPO, as measured by testing TPO-synergism to adenosine diphosphate in platelet activation or by megakaryocyte colony assays. Flow cytometric analysis revealed absent surface expression of the TPO receptor c-Mpl in 3 of 3 patients. Sequence analysis of the c-mpl gene revealed point mutations in 8 of 8 patients: We found frameshift or nonsense mutations that are predicted to result in a complete loss of c-Mpl function in 5 patients. Heterozygous or homozygous missense mutations predicted to lead to amino acid exchanges in the extracellular domain of the receptor were found in 3 other patients. The type of mutations correlated with the clinical course of the disease. We propose a defective c-Mpl expression due to c-mpl mutations as the cause for thrombocytopenia and progression into pancytopenia seen in patients with CAMT.
Extraction and Characteristics of Anti-obesity Lipase Inhibitor fromPhellinus linteus (2010)

To develop a potent anti-obesity lipase inhibitor from mushroom, the lipase inhibitory activities of various mushroom extracts were determined. Methanol extracts from Phellinus linteus fruiting body exhibited the highest lipase inhibitory activity (72.8%). The inhibitor was maximally extracted by treatment of a P. linteus fruiting body with 80% methanol at 40℃ for 24 hr. After partial purification by systematic solvent extraction, the inhibitor was stable in the range of 40~80℃ and pH 2.0~9.0. In addition to lipase inhibitory activity, the inhibitor showed 59.4% of superoxide dismutase-like activity and 56.3% of acetylcholinesterase inhibitory activity.
Exponential Current Pulse Generation for Efficient Very High-Impedance Multisite Stimulation (2010)

We describe in this paper an intracortical current-pulse generator for high-impedance microstimulation. This dual-chip system features a stimuli generator and a high-voltage electrode driver. The stimuli generator produces flexible rising exponential pulses in addition to standard rectangular stimuli. This novel stimulation waveform is expected to provide superior energy efficiency for action potential triggering while releasing less toxic reduced ions in the cortical tissues. The proposed fully integrated electrode driver is used as the output stage where high-voltage supplies are generated on-chip to significantly increase the voltage compliance for stimulation through high-impedance electrode-tissue interfaces. The stimuli generator has been implemented in 0.18-μm CMOS technology while a 0.8-μm CMOS/DMOS process has been used to integrate the high-voltage output stage. Experimental results show that the rectangular pulses cover a range of 1.6 to 167.2 μA with a DNL and an INL of 0.098 and 0.163 least-significant bit, respectively. The maximal dynamic range of the generated exponential reaches 34.36 dB at full scale within an error of ± 0.5 dB while all of its parameters (amplitude, duration, and time constant) are independently programmable over wide ranges. This chip consumes a maximum of 88.3 μ W in the exponential mode. High-voltage supplies of 8.95 and -8.46 V are generated by the output stage, boosting the voltage swing up to 13.6 V for a load as high as 100 kΩ.
Mathematics of quantitative kinetic PCR and the application of standard curves (2003)

Fluorescent monitoring of DNA amplification is the basis of real-time PCR, from which target DNA concentration can be determined from the fractional cycle at which a threshold amount of amplicon DNA is produced. Absolute quantification can be achieved using a standard curve constructed by amplifying known amounts of target DNA. In this study, the mathematics of quantitative PCR are examined in detail, from which several fundamental aspects of the threshold method and the application of standard curves are illustrated. The construction of five replicate standard curves for two pairs of nested primers was used to examine the reproducibility and degree of quantitative variation using SYBER Green I fluorescence. Based upon this analysis the application of a single, well- constructed standard curve could provide an estimated precision of +/-6-21%, depending on the number of cycles required to reach threshold. A simplified method for absolute quantification is also proposed, in which quantitative scale is determined by DNA mass at threshold.
Accelerating concurrent hardware design with behavioural modelling and system simulation (1995)

This paper describes a functional hardware verification methodology for ASIC intensive products. It spans the ASIC, board, and system level, enabling simulation of the design concurrent with ASIC and board development. The simulation strategy relies on rapid development of behavioural models of ASICs to enable work to proceed in parallel and to achieve the necessary simulation efficiency. The results from a project on which the methodology was used are presented. The process provided early visibility of over 200 issues in the system of which 32 were critical to the successful conformance and timely completion of the project.
Combination of multiple searches (1994)

The TREC-2 project at Virginai Tech focused on methods for combining the evidence from multiple retrieval runs to improve performance over any single retrieval method. This paper describes one such method that has been shown to increase performance by combining the similarity values from five different retrieval runs using both vector space and P-norm extended boolean retrieval methods
Expression of the inlAB operon by Listeria monocytogenes is not required for entry into hepatic cells in vivo (1996)

Listeria monocytogenes injected intravenously into mice is taken up in the liver, where hepatocytes serve as the principal site of intracellular replication. The factors effecting entry of L. monocytogenes into hepatic cells remain to be determined. Others have shown that the protein products of the inlAB (internalin) operon are required for maximum entry of L. monocytogenes into a number of cell lines in vitro. Likewise, we report here that expression of the inlAB operon was required for maximum uptake of L. monocytogenes by primary cultures of mouse hepatocytes. Uptake of an inlAB mutant strain of L. monocytogenes was approximately 10-fold less than that of the isogenic wild-type control. In contrast, inlAB expression was not a factor in (i) clearance of L. monocytogenes injected intravenously into mice and taken up in the liver, (ii) the distribution of L. monocytogenes among hepatocytes and nonparenchymal cells in the liver, or (iii) internalization of L. monocytogenes by hepatic cells in vivo. These latter findings suggest that infection of hepatic cells by L. monocytogenes in vivo does not require the protein products of the inlAB operon.
Cooperative Binding of 8-mer Oligonucleotides Containing 5-(1-Propynyl)-2'-deoxyuridine to Adjacent DNA Sites by Triple-Helix Formation (1994)

The design of cooperative domains between DNA-binding&#13;\nligands for modulation of the kinetics and thermodynamics of&#13;\nligand- nucleic acid interactions is at an early stage of development. Pyrimidine oligodeoxyribonucleotides 11 nucleotides&#13;\n(nt) in length are known to bind cooperatively to abutting sites&#13;\non double-helical DNA by triple-helix formation, resulting in a&#13;\n20-fold increase in the association constant for one oligonucleotide&#13;\nin the presence of the neighboring oligonucleotide. This 1.8&#13;\nkcal·mol^(-1) interaction energy may arise from π-stacking between&#13;\nthe bases at the triple-helical junction. The incorporation of&#13;\ndiscrete dimerization domains to oligonucleotides such as those&#13;\ncapable of forming a short Watson-Crick helix produces a&#13;\ncooperative interaction and results in a 44-fold enhancement in&#13;\nthe association constant of one oligonucleotide in the presence of&#13;\na neighbor.
A Model of Structure and Catalysis for Ketoreductase Domains in Modular Polyketide Synthases (2002)

A putative catalytic triad consisting of tyrosine, serine, and lysine residues was identified in the ketoreductase (KR) domains of modular polyketide synthases (PKSs) based on homology modeling to the short chain dehydrogenase/reductase (SDR) superfamily of enzymes. This was tested by constructing point mutations for each of these three amino acid residues in the KR domain of module 6 of the 6-deoxyerythronolide B synthase (DEBS) and determining the effect on ketoreduction. Experiments conducted in vitro with the truncated DEBS Module 6+TE (M6+TE) enzyme purified from Escherichia coli indicated that any of three mutations, Tyr --> Phe, Ser --> Ala, and Lys --> Glu, abolish KR activity in formation of the triketide lactone product from a diketide substrate. The same mutations were also introduced in module 6 of the full DEBS gene set and expressed in Streptomyces lividans for in vivo analysis. In this case, the Tyr --> Phe mutation appeared to completely eliminate KR6 activity, leading to the 3-keto derivative of 6-deoxyerythronolide B, whereas the other two mutations, Ser --> Ala and Lys --> Glu, result in a mixture of both reduced and unreduced compounds at the C-3 position. The results support a model analogous to SDRs in which the conserved tyrosine serves as a proton donating catalytic residue. In contrast to deletion of the entire KR6 domain of DEBS, which causes a loss in substrate specificity of the adjacent acyltransferase (AT) domain in module 6, these mutations do not affect the AT6 specificity and offer a potentially superior approach to KR inactivation for engineered biosynthesis of novel polyketides. The homology modeling studies also led to identification of amino acid residues predictive of the stereochemical nature of KR domains. Finally, a method is described for the rapid purification of engineered PKS modules that consists of a biotin recognition sequence C-terminal to the thioesterase domain and adsorption of the biotinylated module from crude extracts to immobilized streptavidin. Immobilized M6+TE obtained by this method was over 95% pure and as catalytically effective as M6+TE in solution.
<title>JPEG 2000 for efficient imaging in a client/server environment</title> (2001)

The JPEG 2000 image compression system offers significant opportunity to improve imaging over the Internet. The JPEG 2000 standard is ideally suited to the client/server architecture of the web. With only one compressed version stored, a server can transmit an image with the resolution, quality, size, and region custom specified by an individual client. It can also serve an interactive zoom and pan client application. All of these can be achieved without server side decoding while using only minimal server computation, storage, and bandwidth. This paper discusses some of the system issues involved in Internet imaging with JPEG 2000. The choices of the client, passing of control information, and the methods a server could use to serve the client requests are presented. These issues include use of JPEG 2000 encoding and the decoding options in the standard. Also, covered are some proposed techniques that are outside the existing standards.