gebhart/patrepeval huggingface dataset:
NO‐ and haem‐independent activation of soluble guanylyl cyclase: molecular basis and cardiovascular implications of a new pharmacological principle (2002)

1. Soluble guanylyl cyclase (sGC) is the only proven receptor for the ubiquitous biological messenger nitric oxide (NO) and is intimately involved in many signal transduction pathways, most notably in regulating vascular tone and platelet function. sGC is a heterodimeric (alpha/ss) protein that converts GTP to cyclic GMP; NO binds to its prosthetic haem group. Here, we report the discovery of a novel sGC activating compound, its interaction with a previously unrecognized regulatory site and its therapeutic implications. 2. Through a high-throughput screen we identified BAY 58-2667, an amino dicarboxylic acid which potently activates sGC in an NO-independent manner. In contrast to NO, YC-1 and BAY 41-2272, the sGC stimulators described recently, BAY 58-2667 activates the enzyme even after it has been oxidized by the sGC inhibitor ODQ or rendered haem deficient. 3. Binding studies with radiolabelled BAY 58-2667 show a high affinity site on the enzyme. 4. Using photoaffinity labelling studies we identified the amino acids 371 (alpha-subunit) and 231 - 310 (ss-subunit) as target regions for BAY 58-2667. 5. sGC activation by BAY 58-2667 results in an antiplatelet activity both in vitro and in vivo and a potent vasorelaxation which is not influenced by nitrate tolerance. 6. BAY 58-2667 shows a potent antihypertensive effect in conscious spontaneously hypertensive rats. In anaesthetized dogs the hemodynamic effects of BAY 58-2667 and GTN are very similar on the arterial and venous system. 7. This novel type of sGC activator is a valuable research tool and may offer a new approach for treating cardiovascular diseases.
Bilateral Carpal Tunnel Release at One Operation (1992)

THE DIVISION OF the transverse carpal ligament for relief of carpal tunnel syndrome has been a standard operative procedure since the early 1950s. The surgical technique, however, is controversial, and the literature is rife with individual preferences and biases. This report describes our results in 228 tensor fasciae patients who underwent a bilateral carpal ligament release in one procedure that used a small transverse incision in the wrist crease. When the patients' responses to a questionnaire, records of postoperative visits, and follow-up phone calls were analyzed, 216 patients (94.7%) had a satisfactory outcome. One hundred fifty-five patients were working before surgery, with 88.3% back to work in 6 weeks or less. There were no major complications.
What is the role of α‐linolenic acid for mammals? (2002)

This review examines the data pertaining to an important and often underrated EFA, alpha-linolenic acid (ALA). It examines its sources, metabolism, and biological effects in various population studies, in vitro, animal, and human intervention studies. The main role of ALA was assumed to be as a precursor to the longer-chain n-3 PUFA, EPA and DHA, and particularly for supplying DHA for neural tissue. This paper reveals that the major metabolic route of ALA metabolism is beta-oxidation. Furthermore, ALA accumulates in specific sites in the body of mammals (carcass, adipose, and skin), and only a small proportion of the fed ALA is converted to DHA. There is some evidence that ALA may be involved with skin and fur function. There is continuing debate regarding whether ALA has actions of its own in relation to the cardiovascular system and neural function. Cardiovascular disease and cancer are two of the major burdens of disease in the 21st century, and emerging evidence suggests that diets containing ALA are associated with reductions in total deaths and sudden cardiac death. There may be aspects of the action and, more importantly, the metabolism of ALA that need to be elucidated, and these will help us understand the biological effects of this compound better. Additionally, we must not forget that ALA is part of the whole diet and should be seen in this context, not in isolation.
Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. (1990)

Prior to constructing a library of yeast artificial chromosomes (YACs) containing very large human DNA fragments, we performed a series of preliminary experiments aimed at developing a suitable protocol. We found an inverse relationship between YAC insert size and transformation efficiency. Evidence of occasional rearrangement within YAC inserts was found resulting in clonally stable internal deletions or clonally unstable size variations. A protocol was developed for preparative electrophoretic enrichment of high molecular mass human DNA fragments from partial restriction digests and ligation with the YAC vector in agarose. A YAC library has been constructed from large fragments of DNA from an Epstein-Barr virus-transformed human lymphoblastoid cell line. The library presently contains 50,000 clones, 95% of which are greater than 250 kilobase pairs in size. The mean YAC size of the library, calculated from 132 randomly isolated clones, is 430 kilobase pairs. The library thus contains the equivalent of approximately seven haploid human genomes.
Suicide in major depression (1994)

Although about half of the suicide victims with major depression were receiving psychiatric care at the time of death, few were receiving adequate treatment for depression. There were significant sex differences in current and previous treatment and suicide methods. For suicide prevention in major depression, it would seem crucial to improve treatment and follow-up, for males with major depression, in particular.
Targeting the glucocorticoid receptor in breast and prostate cancers (2015)

Steroid receptors for androgens and estrogens have essential roles in prostate and breast cancers. Recently, glucocorticoid receptor (GR) activity has also been proposed as having an important role in these cancers. Underscoring the cooperative nature of nuclear receptor activity, data now suggest that GR function in prostate and breast cancers is dependent on the tumor's concomitant androgen or estrogen receptor activity.
Atomic Layer Deposition of Tungsten Nitride Films Using Sequential Surface Reactions (2000)

Tungsten nitride films were deposited with atomic layer control using sequential surface reactions. The tungsten nitride film growth was accomplished by separating the binary reaction into two half‐reactions. Successive application of the and half‐reactions in an ABAB... sequence produced tungsten nitride deposition at substrate temperatures between 600 and 800 K. Transmission Fourier transform infrared (FTIR) spectroscopy monitored the coverage of and surface species on high surface area particles during the and half‐reactions. The FTIR spectroscopic results demonstrated that the and half‐reactions were complete and self‐limiting at temperature ≥600 K. In situ spectroscopic ellipsometry monitored the film growth on Si(100) substrates vs. temperature and reactant exposure. A tungsten nitride deposition rate of 2.55 Å/AB cycle was measured at 600–800 K for and reactant exposures ≥3000 L and 10,000 L, respectively. X‐ray photoelectron spectroscopy depth‐profiling experiments determined that the films had a stoichiometry with low C and O impurity concentrations. X‐ray diffraction investigations revealed that the tungsten nitride films were microcrystalline. Atomic force microscopy measurements of the deposited films observed remarkably flat surfaces indicating smooth film growth. These smooth tungsten nitride films deposited with atomic layer control should be useful as diffusion barriers for Cu on contact and via holes. © 2000 The Electrochemical Society. All rights reserved.
Structural domains of heparan sulphate for specific recognition of the C-terminal heparin-binding domain of human plasma fibronectin (HEPII) (1996)

Heparan sulphate (HS) is an abundant polysaccharide component of the pericellular domain and is found in most soft tissues and all adherent cells in culture. It interacts with a wide spectrum of proteins including polypeptide growth factors and glycoproteins of the extracellular matrix. These interactions might influence fundamental cellular activities such as adhesion, growth and migration. HS might therefore represent a highly adaptive mechanism by which cells respond to their environment. The present study shows that the interaction between fibroblast HS, metabolically labelled with [3H]glucosamine, and the C-terminal heparin-binding domain of human plasma fibronectin (HEPII), is determined by distinct regions of the polysaccharide chain. By using a very sensitive affinity-chromatography method and specific polysaccharide scission it was shown that the HEPII-binding regions of HS reside within sulphated domains that are resistant to degradation by heparinase III. In addition, optimal binding was achieved with specific heparinase III-resistant fragments of 14-16 monosaccharides in length. The affinity of HS for HEPII was significantly decreased when the polysaccharide was cleaved with heparinase I. Chondroitin sulphate and dermatan sulphate were poor competitive inhibitors of [3H]HS binding to HEPII whereas unlabelled HS and heparin gave a strong inhibitory activity, with heparin being the most potent inhibitor. These findings suggest that the interaction between HEPII and HS is specific and requires extended sequences of seven to eight N-sulphated disaccharides in which a proportion of the iduronate residues are sulphated at C-2. The results have important implications for the functions of HS in cell adhesion and migration.
Second Harmonic Generation Properties of Fluorescent Polymer-Encapsulated Gold Nanoparticles (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSecond Harmonic Generation Properties of Fluorescent Polymer-Encapsulated Gold NanoparticlesHeather A. Clark, Paul J. Campagnola, Joseph P. Wuskell, Aaron Lewis, and Leslie M. LoewView Author Information Center for Biomedical Imaging Technology and Department of Physiology University of Connecticut Health Center Farmington, Connecticut 06030 Division of Applied Physics, Hebrew University Jerusalem, Israel Cite this: J. Am. Chem. Soc. 2000, 122, 41, 10234–10235Publication Date (Web):September 28, 2000Publication History Received22 June 2000Published online28 September 2000Published inissue 1 October 2000https://pubs.acs.org/doi/10.1021/ja002223vhttps://doi.org/10.1021/ja002223vrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1155Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Dyes and pigments,Gold,Metal nanoparticles,Nanoparticles,Nonlinear optics Get e-Alerts
Engineered voltage-responsive nanopores (2009)

The creation of synthetic devices that mimic functionality of biological systems is a task of fundamental importance for the future development of bio- and nanotechnology and also an ultimate test of our understanding of the biological systems. Among a plethora of bio-inspired devices, designed nanopores and nanochannels with an embedded functionality are of particular interest because of their potential applications in nanofluidic electronics, biosensing, separation, synthetic biology, and single-molecule manipulation. In this respect, nanopores with built-in stimulus-responsive properties are of special benefit. A transmembrane potential is a particularly useful stimulus as it is non-invasive, tunable, and can act over a short time scale. This critical review considers engineered solid-state and protein nanopores with voltage-responsive properties. The engineered systems show nonlinear current-voltage curves, and/or voltage-dependent switching between discrete conductance states (141 references).
Digital optical networks using photonic integrated circuits (PICs) address the challenges of reconfigurable optical networks (2008)

To reduce costs and simplify operations, carriers are deploying flexible optical networks that can be easily reconfigured and managed remotely. This article provides an overview of typical all-optical reconfigurable optical add/drop multiplexer (ROADM) systems and their associated network issues. We describe a novel digital optical network architecture based on digital ROADM systems, which use photonic integrated circuits (PICs) to overcome many of these issues. Digital ROADM systems use monolithic PICs to integrate over 60 discrete optical components, including lasers, modulators and detectors, into a single pair of optical components, allowing cost-effective optical-electrical-optical conversion at every node. This also allows key functions such as service reconfiguration, add/drop and protection to be implemented in the digital domain, and enables de-coupling of service provisioning from optical link engineering, termed bandwidth virtualization. Finally, key deployment, reliability and operational metrics for PIC-based digital ROADM systems are presented.
Programming for graphics files in C and C (1994)

From the Publisher:
Even the best programmers can find the details of handling graphics files to be a tricky and often frustrating experience. You have to resolve memory management, format conversion, file compression, and a host of other complex issues before you can even think about reading, writing, and processing graphics files. This technical source book gives C and C++ programmers the tools they need to incorporate efficient graphics-handling routines in their code, including compression techniques for very large graphics files, with detailed analyses of the most popular and innovative schemes, including JPEG; memory-management strategies for segmented (DOS) and virtual (Macintosh, UNIX, NT, and OS/2) environments; algorithms and code for format conversions, as well as a guide to reconciling file formats with printer formats; a special framework for bitmap files; and platform-independent C and C++ source code.
A Phase II Study of Troglitazone, an Activator of the PPAR?? Receptor, in Patients with Chemotherapy-Resistant Metastatic Colorectal Cancer (2002)

Troglitazone is not an active agent for the treatment of metastatic colorectal cancer.
Effect of TRH analogs on neurologic recovery after experimental spinal trauma (1985)

Effects of two stable TRH analogs were compared after experimental spinal trauma in cats. CG3509, which has a substitution at the amino-terminus of the peptide, improved neurologic recovery; MK771, which has modifications at both ends of the tripeptide, was ineffective. These findings indicate that TRH analogs may be of use in spinal cord injury, and suggest that the C-terminal amino acid may be critical for the beneficial effects of TRH or TRH-analogs in CNS injury.
Constitutive Overexpression of Secreted Heterologous Proteins Decreases Extractable BiP and Protein Disulfide Isomerase Levels in Saccharomyces cerevisiae (1995)

High-level gene expression does not always lead to corresponding high-level secretion of heterologous proteins in yeast. The rate-limiting step in many cases has been shown to exit from the endoplasmic reticulum (ER). Within the ER, the correct folding of secreted proteins is required for export competence; hence, the cellular proteins involved in these events are likely to be important for efficient secretion. We have found that the extractable levels of two ER-resident proteins involved in folding--heavy chain binding protein (BiP) and protein disulfide isomerase (PDI)--are significantly reduced by prolonged constitutive overexpression of human granulocyte colony stimulating factor (GCSF), human erythropoietin, or Schizosaccharomyces pombe acid phosphatase. However, the rate of BiP synthesis measured in pulse--chase radiolabeling experiments is not reduced by GCSF overexpression, and galactose-directed transcription of the BiP gene does not restore normal BiP protein levels once they have been depleted. The observed loss of lumenal resident proteins, either by proteolysis or irreversible aggregation, is expected to contribute significantly to the inefficiency of foreign protein secretion in yeast.
Intralipid infusion abolishes ability of human serum to cholesterol-load cultured macrophages. (1989)

Intralipid is widely used for intravenous alimentation and contains triglyceride-emulsion particles and phospholipid liposomes. After infusion, triglyceride-emulsion particles resemble chylomicron remnants and thus may be atherogenic. On the other hand, intravenous infusion of phospholipid liposomes produces regression of experimental atherosclerosis and abolishes the ability of hypercholesterolemic rabbit plasma to cholesterol-load cultured macrophage foam cells. To determine the net effect of intralipid infusion on cellular cholesterol balance, J-774 macrophages were incubated for 18 hours with human serum obtained before, during, and after a 6-hour infusion of 10% Intralipid. Compared to serum-free medium, pre-infusion serum increased cellular unesterified cholesterol by 76% and cholesteryl ester by 78%. In contrast, serum obtained after the 6-hour infusion reduced cellular unesterified cholesterol by 23% and cholesteryl ester by 15%. Serum obtained 18 hours after the end of the infusion still showed impaired cholesterol-loading ability. Mouse peritoneal macrophages incubated with these serum samples behaved similarly. Compared to pre-infusion serum, postinfusion serum inhibited cellular uptake of 125I-low density lipoprotein and 125I-very low density lipoprotein by 50% and 80%, respectively, and also enhanced the efflux of cellular cholesterol by 46%. We conclude that the ability of human serum to cause cholesterol accumulation in cultured macrophages is abolished by an infusion of Intralipid. This effect is mediated by a reduction in cholesterol uptake by the cells and by an increase in cell cholesterol efflux. If similar events occur in the arterial wall, Intralipid infusion might inhibit foam cell formation in vivo.
A Phase I Study of Topical Tempol for the Prevention of Alopecia Induced by Whole Brain Radiotherapy (2004)

This study demonstrates that topical application of Tempol to the scalp before whole brain radiation is safe and well tolerated. Evidence of protection against radiation-induced alopecia was observed. A phase II study that uses a gel formulation to increase the exposure of scalp to Tempol has been initiated.
Parallel and Distributed Processing Techniques and Applications: The 2013 WorldComp International Conference Proceedings (2014)

Parallel and Distributed Processing Techniques and Applications is a compendium of articles and papers that were presented at PDPTA '13, an international conference that serves researchers, scholars, professionals, students, and academicians. Hamid R. Arabnia, Hiroshi Ishii, Minoru Ito, Hiroaki Nishikawa, Fernando G. Tinetti, George A. Gravvanis, George Jandieri, and Ashu M. G. Solo (Editors) Selected topics include parallel and distributed algorithms and applications, mathematical modeling and problem solving, and grid + cloud computing and supporting tools + applications, etc. Selected Sessions and Topics for example: Resource Allocation, Scheduling, Energy-Aware Computing + Load-Balancing + Fault-Tolerant Systems Parallel and Distributed Algorithms and Applications Mathematical Modeling and Problem Solving - MPS Grid + Cloud Computing and Supporting Tools + Applications Systems Software + Programming Models + Threads + Caching + File Systems + Testing and Monitoring Methods Performance Evaluation, Estimation, and Related Issues Communication Systems + Networks and Interconnection Networks + Peer-To-Peer Networks Ad-Hoc Networks + Sensor Networks and Applications Cluster Computing + Multi-Core, GPU, FPGA Processing and Applications Data-Driven Networking Systems with High Tolerance for Disaster, Fault and Congestion Position and Regular - Communication Systems, Cloud Computing, Reconfigurable Systems, Parallel and Distributed Computing, Scheduling, Architectures, and Applications Cloud Computing, Optimization, Sensor Networks, Scheduling, and Applications
Identification of a novel peptide motif that mediates cross‐linking of proteins to cell walls (1999)

A cDNA clone representing a member of a novel class of cell wall proteins was isolated from tobacco plants. We have designated this protein NtTLRP for tyrosine- and lysine-rich protein. It is structurally related to the previously identified TLRP from tomato plants, sharing a high amino-acid sequence similarity at the C-terminal region. This region contains what appears to be a novel peptide motif which we call CD for cysteine-rich domain, and which is common to several other cell-wall proteins. By using a functional test in transgenic plants, we demonstrate that the presence of the CD domain is per se sufficient to cross-link previously soluble proteins to the cell wall. We present evidence that NtTLRP is cross-linked and specifically localizes to the cell wall of lignified cells. The highly localized deposition of NtTLRP in these cells indicates that this class of cell-wall proteins may have a specialized function in the formation of xylem tissue.
Synergistic fibrinolysis: combined effects of plasminogen activators and an antibody that inhibits alpha 2-antiplasmin. (1990)

To improve the efficacy of plasminogen activators, we produced a monoclonal antibody (RWR) that inhibits human alpha 2-antiplasmin (alpha 2AP). In addition to inhibiting alpha 2AP in plasma, RWR binds to and inhibits fibrin cross-linked alpha 2AP and reproduces the "spontaneous" clot lysis that is the hallmark of human alpha 2AP deficiency. By inhibiting the inactivation of plasmin by alpha 2AP, RWR interacts synergistically with plasminogen activators to increase the potency (for 50% clot lysis) of urokinase by 80-fold, tissue plasminogen activator by 27-fold, and streptokinase by 20-fold. Yet, for a given amount of fibrinolysis, the combination of RWR and lower doses of plasminogen activator leads to less fibrinogen consumption than is obtained with higher, equipotent doses of plasminogen activator alone. These results suggest a strategy for increasing the efficacy of plasminogen activators. More generally, this approach to amplifying enzymatic activity by immunoneutralizing an inhibitor may be useful in other biologic processes that are rigidly governed by inhibitors.