gebhart/patrepeval huggingface dataset:
Detection and Duration of Porcine Reproductive and Respiratory Syndrome Virus in Semen, Serum, Peripheral Blood Mononuclear Cells, and Tissues from Yorkshire, Hampshire, and Landrace Boars (2001)

Because transmission of porcine reproductive and respiratory syndrome virus (PRRSV) can occur through boar semen, it is important to identify persistently infected boars. However, even for boars given the same PRRSV strain and dose, variability in the duration of viral shedding in semen has been observed, suggesting that host factors are involved in PRRSV persistence. To determine whether there are host genetic factors, particularly litter and breed differences related to the persistence of PRRSV, 3 litters from 3 purebred swine breeds were used for this study. It was also determined whether PRRSV could be detected for a longer period of time in serum, semen, or peripheral blood mononuclear cells (PBMC) and if PRRSV could still be detected in tissues after these antemortem specimens were PRRSV negative for a minimum of 2-3 weeks. Three Hampshire, 3 Yorkshire, and 2 Landrace PRRSV-naive boars were obtained and inoculated intranasally with a wild-type PRRSV isolate (SD-23983). All boars within each breed were from the same litter, and litters were within 9 days of age. Serum and PBMC were collected twice weekly from each boar and analyzed for the presence of PRRSV by virus isolation and the polymerase chain reaction (PCR). Serum was also used to obtain virus neutralization titers and enzyme-linked immunosorbent assay S/P values. Semen was collected twice weekly from 7 of 8 boars and analyzed by PCR. After all specimens were PRRSV negative for a minimum of 2-3 weeks, each boar was euthanized, and 21 tissues plus saliva, serum, feces, and urine were collected. All postmortem specimens were evaluated by virus isolation. Specimens that were PRRSV negative by virus isolation were then evaluated by PCR. The mean number of days (+/-SD) for the duration of PRRSV shedding in semen was 51+/-26.9 days, 7.5+/-4.9 days, and 28.3+/-17.5 days for Landrace, Yorkshire, and Hampshire boars, respectively. Because of small sample sizes and large SDs, the differences in duration of PRRSV shedding in semen between breeds were not considered significant. However, the trend suggested that Yorkshire boars were more resistant to PRRSV shedding in semen than were Landrace boars, requiring further investigation using a larger numbers of boars. PRRSV was detected for a longer period in semen than in serum or PBMC in 4 of 7 boars. Viremia could be detected for a longer period in serum than in PBMC in 6 of 8 boars. After a minimum of 2-3 weeks of PRRSV-negative serum, semen, and PBMC, PRRSV could still be detected in the tonsil of 3 of 8 boars by virus isolation, indicating that boars still harbor PRRSV within the tonsil even though antemortem specimens are PRRSV negative.
Gonadotropin-releasing hormone neurons in the rhesus macaque are not immunoreactive for the estrogen receptor (1995)

The issue of whether gonadotropin-releasing hormone (GnRH) neurons in the primate contain the estrogen receptor was examined by immunocytochemistry using prepubertal and adult (intact and ovariectomized) female rhesus macaques. No GnRH neurons were found to contain nuclei that were immunoreactive for the estrogen receptor. These results confirm in primates what has been reported in other species and leave open the question of how the effects of gonadal steroids on GnRH neurons are mediated.
Self-supervised Monocular Road Detection in Desert Terrain (2006)

We present a method for identifying drivable surfaces in difficult unpaved and offroad terrain conditions as encountered in the DARPA Grand Challenge robot race. Instead of relying on a static, pre-computed road appearance model, this method adjusts its model to changing environments. It achieves robustness by combining sensor information from a laser range finder, a pose estimation system and a color camera. Using the first two modalities, the system first identifies a nearby patch of drivable surface. Computer Vision then takes this patch and uses it to construct appearance models to find drivable surface outward into the far range. This information is put into a drivability map for the vehicle path planner. In addition to evaluating the method's performance using a scoring framework run on real-world data, the system was entered, and won, the 2005 DARPA Grand Challenge. Post-race log-file analysis proved that without the Computer Vision algorithm, the vehicle would not have driven fast enough to win.
Transcranial Infrared Laser Therapy Improves Clinical Rating Scores After Embolic Strokes in Rabbits (2004)

This study shows that laser treatment improved behavioral performance if initiated within 6 hours of an embolic stroke and the effect of laser treatment is durable. Therefore, transcranial laser treatment may be useful to treat human stroke patients and should be further developed.
Impaired Perfusion Following Cerebrovascular Stasis (1973)

The possibility that a vascular phenomenon might contribute to the brain's inability to survive ischemia for more than several minutes was initially suggested by observations such as those of Ames and Gurian<sup>1</sup>and Neely and Youmans.<sup>2</sup>The former noted that isolated rabbit retina and optic nerve recovered nearly normal electrical activity following a 20-minute deprivation of oxygen and glucose at 37 C. The latter group demonstrated recovery of dogs from periods of up to 25 minutes of total cerebral ischemia, when produced by intrathecal infusion of saline to a pressure of 400 mm Hg resulting in exsanguination of the brain. This report summarizes the pertinent work done by the group based mostly at the Massachusetts General Hospital, demonstrating and characterizing an impairment of cerebrovascular perfusion, which begins within the first five to ten minutes of total cerebral circulatory arrest. In addition, it discusses the mechanisms that have thus
Changes in Auxin Response from Mutations in an AUX/IAA Gene (1998)

Transcription of the AUX/IAA family of genes is rapidly induced by the plant hormone auxin, but evidence that AUX/IAA genes mediate further responses to auxin has been elusive. Changes in diverse auxin responses result from mutations in the Arabidopsis AXR3 gene. AXR3 was shown to be a member of the AUX/IAA family, providing direct evidence that AUX/IAA genes are central in auxin signaling. Molecular characterization of axr3 gain-of-function and loss-of-function mutations established the functional importance of domains conserved among AUX/IAA proteins.
Signs of the presence of Th17 cells in chronic periodontal disease (2012)

The results demonstrated a significant increase in the number of some specific markers of Th17 cells in patients suffering from periodontal disease in comparison with normal control subjects.
Cloning and characterization of a testis-specific thymosin beta 10 cDNA. Expression in post-meiotic male germ cells. (1991)

Thymosin beta 10 is one of a small family of proteins closely related in sequence to thymosin beta 4, recently identified as an actin-sequestering protein. A single molecular weight species of thymosin beta 10 mRNA is present in a number of rat tissues. In adult rat testis, an additional thymosin beta 10 mRNA of higher molecular weight was identified. Nucleotide sequencing of cDNA clones complementary to the testis-specific thymosin mRNA indicated that this mRNA differed from the ubiquitous thymosin beta 10 mRNA only in its 5'-untranslated region, beginning 14 nucleotides upstream of the translation initiation codon. These results, together with primer extension experiments, suggest that the two thymosin beta 10 mRNAs are transcribed from the same gene through a combination of differential promoter utilization and alternative splicing. The novel thymosin beta 10 mRNA could be detected only in RNA isolated from sexually mature rat testis. Both mRNAs were present in pachytene spermatocytes; only the testis-specific mRNA was detected in postmeiotic haploid spermatids. Immunoblot analysis using specific antibodies showed that the thymosin beta 10 protein synthesized in adult testis was identical in size to that synthesized in brain. Immunohistochemical analysis showed that the protein was present in differentiating spermatids, suggesting that the testis-specific thymosin beta 10 mRNA is translated in haploid male germ cells.
Monte Carlo simulation applicable to the growth of rough metal overlayers: Parametric relationships related to the electrochemical roughening (1990)

Monte Carlo simulations of growth modes of overlayers built up from a finite number of particles randomly distributed in a confined space are presented. These results are applicable to the growth of metal overlayers on a metal substrate, particularly for an understanding of the development and the structure of the rough metal surfaces resulting from the electroreduction of metal-oxide layers. The roughness factor of the deposited overlayers increases with both the number and the sticking probability of depositing particles, and decreases on increasing their surface diffusion length. Otherwise, the roughness factor appears to be practically independent of the number of particles in the critical nucleus. The morphology of the deposits changes gradually from that of a compact cluster arrangement to that of a dendritic one. Correspondingly, the roughness factor increases considerably as either the sticking probability increases or the diffusion length decreases. Data obtained for the electrochemical growth of Au and Pt overlayers on metal surfaces as derived from combined scanning-tunneling-microscopy and scanning-electron-microscopy imaging are used to test the validity of the conclusions resulting from the Monte Carlo simulation.
Histogram refinement for content-based image retrieval (2002)

Color histograms are widely used for content-based image retrieval. Their advantages are efficiency, and insensitivity to small changes in camera viewpoint. However, a histogram is a coarse characterization of an image, and so images with very different appearances can have similar histograms. We describe a technique for comparing images called histogram refinement, which imposes additional constraints on histogram based matching. Histogram refinement splits the pixels in a given bucket into several classes, based upon some local property. Within a given bucket, only pixels in the same class are compared. We describe a split histogram called a color coherence vector (CCV), which partitions each histogram bucket based on spatial coherence. CCVs can be computed at over 5 images per second on a standard workstation. A database with 15,000 images can be queried using CCVs in under 2 seconds. We demonstrate that histogram refinement can be used to distinguish images whose color histograms are indistinguishable.
Trials of mobile computing applications using circuit-switched cellular and cellular digital packet data (CDPD) (2002)

As the Department of Defense (DoD) lead agency for wireless systems/services, the Defense Information System Agency (DISA) has been involved in multiple wireless activities including trials of wireless commercial products and services. In support of the DoD wireless trials, the MITRE corporation was tasked to evaluate a number of wireless services and technologies for a wireless trial of mobile computing applications. This paper describes several demonstrations that we conducted including wireless e-mail using both circuit-switched cellular and cellular digital packet data (CDPD) and Internet access using CDPD. Many issues related to the integration of different hardware and software packages were resolved and several mobile computing applications were successfully demonstrated. This paper describes our trials in detail and documents the result and the lessons learned from this project.
Emergency Physician–Verified Out‐of‐hospital Intubation: Miss Rates by Paramedics (2004)

The rate of unrecognized, misplaced out-of-hospital intubations in this urban, midwestern setting was 5.8%. This is more consistent with results of prior out-of-hospital studies that used field verification and is discordant with the only other study to exclusively use emergency physician verification performed on arrival to the emergency department.
" The effectiveness of intravenous iron for iron deficiency anemia in gastrointestinal cancer patients: a retrospective study" (2017)

Intravenous administration of iron in DIO patients with ID anemia leads to a significant increase in Hb. Moreover, differentiating between the types of ID anemia based on ferritin levels could be applied to predict therapy response, although better biomarkers are needed.
Multiple Nuclear Localization Sequences Allow Modulation of 5‐Lipoxygenase Nuclear Import (2004)

The nuclear import of proteins typically requires the presence of a nuclear localization sequence (NLS). Some proteins have more than one NLS, but the significance of having multiple NLSs is unclear. The enzyme 5-lipoxygenase (5-LO) has three NLSs that, unlike the tight cluster of basic residues of the classical SV40 large T antigen NLS, contain dispersed basic residues. When attached to green fluorescent protein (GFP), individual 5-LO NLSs caused quantitatively and statistically less import than the SV40 NLS. Combined 5-LO NLSs produced nuclear import that was comparable to that of the SV40 NLS. As expected, GFP/NLS proteins displayed relatively uniform import in all cells. However, a fusion protein of GFP plus the 5-LO protein, modified to contain only one functional NLS, produced some cells with import and some cells without import. A GFP/5-LO fusion protein containing two functional NLSs produced four identifiable levels of nuclear import. Quantitative and visual analysis of a population of cells expressing the intact GFP/5-LO protein, with three intact NLSs, indicated five levels of nuclear import. This suggested that the subcellular distribution of 5-LO may vary widely in normal cells of the body. Consistent with this, immunohistochemical staining of lung sections found that individual macrophages, in situ, displayed cell-specific levels of import of 5-LO. Since nuclear accumulation is known to affect 5-LO activity, multiple NLSs may allow graded regulation of activity via controlled import. Multiple NLSs on other proteins may likewise allow fine control of protein action through modulation of the level of import.
DNA Hybridization Using Surface Plasmon-Coupled Emission (2003)

We describe a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). Excited fluorophores are known to couple with surface oscillations of electrons in thin metal films, typically 50 nm thick silver on a glass prism. These surface plasmons then radiate into the glass at a sharply defined angle determined by the emission wavelength and the optical properties of the glass and metal. This radiation has the same spectral profile as the emission spectrum of the fluorophores. We studied the emission due to Cy3-labeled DNA oligomers bound to complementary unlabeled oligomers which were themselves bound to the metal surface. Hybridization resulted in SPCE due to Cy3-DNA into the prism. Directional SPCE was observed whether the sample was illuminated from the sample side or through the glass substrate at the surface plasmon angle for the excitation wavelength. A large fraction of the total potential emission is coupled to the surface plasmons resulting in improved sensitivity. When illuminated through the prism at the surface plasmon angle, the sensitivity is increased due to the enhanced intensity of the resonance evanescent field. It is known that SPCE depends on proximity to the silver surface. As a result, changes in emission intensity are observed due to fluorophore localization even if hybridization does not affect the quantum yield of the fluorophore. The use of SPCE resulted in suppression of interfering emission from a noncomplementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. We expect SPCE to have numerous applications to nucleic acid analysis and for the measurement of bioaffinity reactions.
Comparison of molecular abnormalities in bronchial brushings and tumor touch preparations (2004)

The current study indicated that detection of molecular abnormalities in bronchial epithelial cells via FISH was very useful in identifying patients at high risk for developing lung carcinoma. The molecular abnormalities identified in the BB/Ts were detected at elevated levels in the tumor specimens.
A pilot trial of paclitaxel, carboplatin, and concurrent radiotherapy for unresectable squamous cell carcinoma of the head and neck. (1997)

Combined chemoradiotherapy is superior to radiotherapy alone for stage III and IV squamous cell carcinoma of the head and neck, and concurrent use of both offers the advantage of synergistic interactions. Our prior trial demonstrated the ease and convenience of administering carboplatin during radiotherapy. Since paclitaxel (Taxol; Bristol-Myers Squibb Company, Princeton, NJ) has activity in squamous cell carcinoma of the head and neck and can act synergistically with both radiotherapy and platinum drugs, we initially added paclitaxel at 45 mg/m2/wk to carboplatin given at 100 mg/m2 during radiotherapy given at conventional fractions. The initial dose of paclitaxel was subsequently reduced to 40 mg/m2/wk. Thirteen of 18 patients entered so far have sufficient follow-up data; 12 are assessable for toxicity and 11 are assessable for response. One died early of progressive disease, two achieved a complete response, six achieved a partial response, and two had stable disease. Toxicities have so far been manageable for the 76 weekly doses administered. Chemotherapy dose reduction was needed in 10 patients. For the planned 100 doses of chemotherapy, 53 (53%) were administered as planned, 23 (23%) were reduced, and 24 (24%) were withheld due to neutropenia or mucositis. There were no toxic deaths, and no patient stopped therapy for toxicity. Paclitaxel/carboplatin can be administered during radiotherapy for squamous cell carcinoma of the head and neck with acceptable toxicities, and further accrual is needed to evaluate the effect of this combination.
Direct identification of sickle cell anemia by blot hybridization. (1981)

Several reports have been published on the use of polymorphisms found in the human hemoglobin genes as a means for prenatal diagnosis of sickle cell anemia. The disadvantages of this approach reside in its limited application and the need for family analysis. Here we report that, by use of restriction endonuclease Dde I and diazobenzyloxymethyl-paper transfer procedures, a direct analysis can be made. Individuals with normal hemoglobin (AA) show two bands (175 and 201 base pairs) complementary to a 5'-specific beta-globin gene probe. Sickle cell trait individuals (AS) exhibit an additional band (376 base pairs). Individuals with sickle cell anemia (SS) show the band at 376 base pairs with a concomitant loss of the 175-base pair band. We interpret these changes in banding pattern to be the result of the elimination of a restriction site for Dde I in the altered codon associated with the sickle cell allele. Because an analysis can be performed on as little as 20 micrograms of cellular DNA, the application to prenatal diagnosis of sickle cell anemia should be possible.
AG robner-Sylvester Hybrid Method for Closed-Form Displacement Analysis of Mechanisms displacement analysis problem for planar and spatial mechanisms can be written as (2000)

a system of multivariate polynomial equations. Elimination theory based on resultants and polynomial continuation are some of the methods that have been used to solve this problem. This paper presents a new approach to displacement analysis using the reduced Grobner basis form of a system of equations under degree lexicographic (dlex) term ordering of its monomials and Sylvester's Dialytic elimination method. Using the Grobner-Sylvester hybrid approach, a finitely solvable system of equations F is trans- formed into its reduced Grobner basis G using dlex term ordering. Next, using the entire or a subset of the set of generators in G, the Sylvester's matrix is assembled. The van- ishing of the resultant, given as the determinant of Sylvester's matrix, yields the necessary condition for polynomials in G (as well as F) to have a common factor. The proposed approach appears to provide a systematic and rational procedure to the problem dis- cussed by Roth, dealing with the generation of (additional) equations for constructing the Sylvester's matrix. Three examples illustrating the applicability of the proposed approach to displacement analysis of planar and spatial mechanisms are presented. The first and second examples address the forward displacement analysis of the general 6-6 Stewart mechanism and the 6-6 Stewart platform, whereas the third example deals with the de- termination of the I/O polynomial of an 8-link 1-DOF mechanism that does not contain any 4-link loop. @S1050-0472~00!01204-6#
Oxidation of fluoroalkyl-substituted carbinols by the Dess-Martin reagent (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOxidation of fluoroalkyl-substituted carbinols by the Dess-Martin reagentRussell J. Linderman and David M. GravesCite this: J. Org. Chem. 1989, 54, 3, 661–668Publication Date (Print):February 1, 1989Publication History Published online1 May 2002Published inissue 1 February 1989https://pubs.acs.org/doi/10.1021/jo00264a029https://doi.org/10.1021/jo00264a029research-articleACS PublicationsRequest reuse permissionsArticle Views4073Altmetric-Citations146LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
CO2 laser-plume interaction in materials processing (2000)

In laser materials processing, localized evaporation caused by focused laser radiation results in a partially-ionized plume above the material surface. The beam is refracted and absorbed as it traverses the plume and these effects are of interest for process development. Here, plume-beam interactions are studied using an axisymmetric, high-temperature gasdynamic model of a plume formed by vapor from a flat iron surface. The beam propagation in the plume is calculated from the paraxial wave equation including absorption and refraction. It is shown that absorption of the beam in the plume has much less direct effect on the power density at the material surface than refraction does. Helium gas is more efficient than argon for reducing the beam refraction and absorption effects. Laser energy reflected from the material surface has significant effects on the plume properties.