gebhart/patrepeval huggingface dataset:
Analysis and design considerations of a load and line independent zero voltage switching full bridge DC/DC converter topology (2002)

The analysis and design of a zero voltage switching (ZVS) full bridge DC/DC power converter topology is presented in this paper. The converter topology presented here employs an asymmetrical auxiliary circuit consisting of a few passive components. With this auxiliary circuit, the full bridge converter can achieve ZVS independent of line and load conditions. The operating principle of the circuit is demonstrated, and the steady state analysis is performed. Based on the analysis, a criterion for optimal design is given. Experiment and simulation on a 350-400 V to 55 V, 500 W prototype converter operated at 100 kHz verify the design and show an overall efficiency of greater than 97% at full load.
The Most Abundant Protein in Bovine Herpes 1 Virions is a Homologue of Herpes Simplex Virus Type 1 UL47 (1991)

The bovine herpesvirus type 1 (BHV-1) protein VP8 is present, in large amounts, in the tegument of virions. As a preliminary step towards determining the function of VP8 and the biological relevance for its abundant presence, we describe the mapping of the location of its gene and determination of its nucleotide sequence. The gene for VP8 was located between 0.088 and 0.108 map units on the BHV-1 genome and contained a 2226 bp reading frame encoding a 742 amino acid protein. The protein, produced in vitro by transcribing and translating the reading frame, was precipitated by monoclonal antibodies and polyclonal serum directed against VP8. The primary structure of VP8 showed considerable homology with the product of the UL47 reading frame of herpes simplex virus type 1.
Role of TLR in B Cell Development: Signaling through TLR4 Promotes B Cell Maturation and Is Inhibited by TLR2 (2005)

The role of TLR4 in mature B cell activation is well characterized. However, little is known about TLR4 role in B cell development. Here, we analyzed the effects of TLR4 and TLR2 agonists on B cell development using an in vitro model of B cell maturation. Highly purified B220(+)IgM(-) B cell precursors from normal C57BL/6 mouse were cultured for 72 h, and B cell maturation in the presence of the TLR agonists was evaluated by expression of IgM, IgD, CD23, and AA4. The addition of LPS or lipid A resulted in a marked increase in the percentage of CD23(+) B cells, while Pam3Cys had no effect alone, but inhibited the increase of CD23(+) B cell population induced by lipid A or LPS. The TLR4-induced expression of CD23 is not accompanied by full activation of the lymphocyte, as suggested by the absence of activation Ag CD69. Experiments with TLR2-knockout mice confirmed that the inhibitory effects of Pam3Cys depend on the expression of TLR2. We studied the effects of TLR-agonists on early steps of B cell differentiation by analyzing IL-7 responsiveness and phenotype of early B cell precursors: we found that both lipid A and Pam3Cys impaired IL-7-dependent proliferation; however, while lipid A up-regulates B220 surface marker, consistent with a more mature phenotype of the IgM(-) precursors, Pam3Cys keeps the precursors on a more immature stage. Taken together, our results suggest that TLR4 signaling favors B lymphocyte maturation, whereas TLR2 arrests/retards that process, ascribing new roles for TLRs in B cell physiology.
Earthshine observations of the Earth's reflectance (2001)

Regular photometric observations of the moon's “ashen light” (earthshine) from the Big Bear Solar Observatory (BBSO) since December 1998 have quantified the earth's optical reflectance. We find large (∼5%) daily variations in the reflectance due to large‐scale weather changes on the other side of the globe. Separately, we find comparable hourly variations during the course of many nights as the earth's rotation changes that portion of the earth in view. Our data imply an average terrestrial albedo of 0.297±0.005, which agrees with that from simulations based upon both changing snow and ice cover and satellite‐derived cloud cover (0.296±0.002). However, we find seasonal variations roughly twice those of the simulation, with the earth being brightest in the spring. Our results suggest that long‐term earthshine observations are a useful monitor of the earth's albedo. Comparison with more limited earthshine observations during 1994–1995 show a marginally higher albedo then.
Surface Science Studies of the Photoactivation of TiO2New Photochemical Processes (2006)

ADVERTISEMENT RETURN TO ISSUEPREVArticleSurface Science Studies of the Photoactivation of TiO2New Photochemical ProcessesTracy L. Thompson and John T. YatesView Author Information Surface Science Center, Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260 Cite this: Chem. Rev. 2006, 106, 10, 4428–4453Publication Date (Web):October 11, 2006Publication History Received11 January 2006Published online11 October 2006Published inissue 1 October 2006https://pubs.acs.org/doi/10.1021/cr050172khttps://doi.org/10.1021/cr050172kresearch-articleACS PublicationsCopyright © 2006 American Chemical SocietyRequest reuse permissionsArticle Views19621Altmetric-Citations1882LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Defects,Heat transfer,Materials,Oxides,Thermodynamic modeling Get e-Alerts
Positively charged sequences of human papillomavirus type 16 capsid proteins are sufficient to mediate gene transfer into target cells via the heparan sulfate receptor (2003)

Using synthetic peptides we have shown that positively charged sequences present at the C terminus of the L1 protein and the N and C termini of the L2 protein of human papillomavirus type 16 (HPV-16) bind to both DNA and heparan sulfate receptors. Moreover, these short amino acid sequences are sufficient to mediate gene transfer in COS-7 cells. The L1 proteins of other HPVs were shown to contain one or two DNA- and heparin-binding sequences that have the capacity to transfer genes. These DNA-binding sequences also recognized the enhancing packaging sequence of bovine papillomavirus type 1. The results suggest that the L2 protein could participate in DNA packaging during maturation of virions.
Serum interleukin‐15 is elevated in systemic lupus erythematosus (2001)

Serum IL-15 may in part be responsible for the immunological abnormalities seen in active SLE.
Tubules-Within-a-Tubule Hierarchical Order of Mesoporous Molecular Sieves in MCM-41 (1996)

The recently discovered mesoporous aluminosilicate MCM-41 consists of hexagonal arrays of nanometer-sized cylindrical pores. It is shown that this material can be synthesized by cooperative condensation of silicate and cylindrical cationic micelles. Careful control of the surfactant-water content and the rate of condensation of silica at high alkalinity resulted in hollow tubules 0.3 to 3 micrometers in diameter. The wall of the tubules consisted of coaxial cylindrical pores, nanometers in size, that are characteristic of those of MCM-41. The formation of this higher order structure may take place through a liquid crystal phase transformation mechanism involving an anisotropic membrane-to-tubule phase change. The hierarchical organization of this "tubules-within-a-tubule" particle texture is similar to that of the frustules of marine diatoms.
Altered Stationary-Phase Response in a Borrelia burgdorferi rpoS Mutant (2000)

The homolog of the chromosomally encoded stationary-phase sigma factor RpoS in Borrelia burgdorferi was inactivated using gyrB(r) as a selectable marker. Two-dimensional nonequilibrium pH gradient electrophoresis of stationary-phase cell lysates identified at least 11 differences between the protein profiles of the rpoS mutant and wild-type organisms. Wild-type B. burgdorferi had a growth phase-dependent resistance to 1 N NaCl, similar to the stationary-phase response reported for other bacteria. The B. burgdorferi rpoS mutant strain was less resistant to osmotic stress in stationary phase than the isogenic rpoS wild-type organism. The results indicate that the B. burgdorferi rpoS homolog influences protein composition and participates in stationary-phase-dependent osmotic resistance. This rpoS mutant will be useful for studying regulation of gene expression in response to changing environmental conditions.
Hyperthyroidism and Hepatic Dysfunction (1992)

Liver dysfunction in hyperthyroid patients has not been well characterized. We analyzed the clinical records of 43 patients with hyperthyroidism to define the spectrum of clinical and liver test abnormalities. The patients were divided into three categories: (a) 18 patients with uncomplicated hyperthyroidism (HT) (b) 19 with hyperthyroidism and congestive heart failure (HT/CHF), and (c) 6 with hyperthyroidism and concomitant unrelated liver disease (HT/ULD). Hepatomegaly and/or spenomegaly were noted in 15 of 19 (79%) patients with HT/CHF as compared to 6 of 18 (33%) patients with HT and 3 of 6 (50%) patients with HT/ULD. Four patients with HT/CHF had ascites. Serum aminotransferase levels greater than 250 IU/L were noted in only 1 of 37 (3%) patients without unrelated liver disease. Patients with HT/ULD or HT/CHF had markedly low prothrombin time. Serum bilirubin levels as high as 323 microM were noted in patients with HT. No characteristic liver histology due to hyperthyroidism was noted. Severe liver test abnormalities, including deep jaundice and prolonged prothrombin time, can occur in patients with hyperthyroidism alone or with HT/CHF. This makes the diagnosis of concomitant, unrelated liver disease difficult until the hyperthyroidism has been controlled.
Modification of Irradiation Injury in Mice and Guinea Pigs by Bone Marrow Injections (1951)

Modification of Irradiation Injury in Mice and Guinea Pigs by Bone Marrow Injections Egon Lorenz, Egon Lorenz National Cancer Institute2Bethesda, Md., and The Division of Biological and Medical Research, Argonne National Laboratory, Chicago, Ill. Search for other works by this author on: Oxford Academic PubMed Google Scholar Delta Uphoff, Delta Uphoff National Cancer Institute2Bethesda, Md., and The Division of Biological and Medical Research, Argonne National Laboratory, Chicago, Ill. Search for other works by this author on: Oxford Academic PubMed Google Scholar T. R. Reid, T. R. Reid National Cancer Institute2Bethesda, Md., and The Division of Biological and Medical Research, Argonne National Laboratory, Chicago, Ill. Search for other works by this author on: Oxford Academic PubMed Google Scholar Emma Shelton Emma Shelton National Cancer Institute2Bethesda, Md., and The Division of Biological and Medical Research, Argonne National Laboratory, Chicago, Ill. Search for other works by this author on: Oxford Academic PubMed Google Scholar 1National Institutes of Health, Public Health Service, Federal Security Agency. Author Notes JNCI: Journal of the National Cancer Institute, Volume 12, Issue 1, August 1951, Pages 197–201, https://doi.org/10.1093/jnci/12.1.197 Published: 01 August 1951 Article history Received: 28 May 1951 Published: 01 August 1951
Williams, John ( c . 1636–1709) (2000)

Tillotson, John Williams, John John Williams was born in Northamptonshire and died in London on 24 April 1709. He was educated at Magdalen Hall, Oxford, graduating BA in 1655 and MA in 1658, and was incorporated at Cambridge in 1660, being awarded a DD there in 1690. Williams became rector of St Mil
Deoxyadenosine analogs induce programmed cell death in chronic lymphocytic leukemia cells by damaging the DNA and by directly affecting the mitochondria (2000)

Abstract Adenine deoxynucleosides induce apoptosis in quiescent lymphocytes and are thus useful drugs for the treatment of indolent lymphoproliferative diseases. To explain why deoxyadenosine and its analogs are toxic to a cell that is not undergoing replicative DNA synthesis, several mechanisms have been proposed, including the direct binding of dATP to the pro-apoptotic factor Apaf-1 and the activation of the caspase-9 and -3 pathways. In this study it is shown, by means of several assays on whole cells and isolated mitochondria, that 2-chloro-2′-deoxyadenosine (2CdA) and 2-choloro-2′-ara-fluorodeoxyadenosine (CaFdA) disrupt the integrity of mitochondria from primary chronic lymphocytic leukemia (B-CLL) cells. The nucleoside-induced damage leads to the release of the pro-apoptotic mitochondrial proteins cytochrome c and apoptosis-inducing factor. The other adenine deoxynucleosides tested displayed comparable DNA-damaging potency but did not affect mitochondrial function. Interference with mitochondrial integrity, thus, may be a factor in the potent cytotoxic effects of 2CdA and CaFdA toward nondividing lymphocytes.
Ocular Distribution, Spectrum of Activity, and In Vivo Viral Neutralization of a Fully Humanized Anti-Herpes Simplex Virus IgG Fab Fragment following Topical Application (2011)

Herpes simplex ocular infection is a major cause of corneal blindness. Local antiviral treatments exist but are associated with corneal toxicity, and resistance has become an issue. We evaluated the biodistribution and efficacy of a humanized anti-herpes simplex virus (anti-HSV) IgG FAb fragment (AC-8; 53 kDa) following repeated topical administration. AC-8 was found in the corneal epithelium, anterior stroma, subepithelial stromal cells, and retinal glial cells, with preferential entry through the ocular limbus. AC-8 was active against 13 different strains of HSV-1, with 50% and 90% mean effective concentrations (MEC(50) and MEC(90), respectively) ranging from 0.03 to 0.13 μg/ml, indicating broad-spectrum activity. The in vivo efficacy of AC-8 was evaluated in a mouse model of herpes-induced ocular disease. Treatment with low-dose AC-8 (1 mg/ml) slightly reduced the ocular disease scores. A greater reduction of the disease scores was observed in the 10-mg/ml AC-8-treated group, but not as much as with trifluridine (TFT). AC-8 treatment reduced viral titers but less than trifluridine. AC-8 did not display any toxicity to the cornea or other structures in the eye. In summary, topical instillation of an anti-HSV FAb can be used on both intact and ulcerated corneas. It is well tolerated and does not alter reepithelialization. Further studies to improve the antiviral effect are needed for AC-8 to be considered for therapeutic use.
Comparative analysis of human and mouse immunoglobulin variable heavy regions from IMGT/LIGM-DB with IMGT/HighV-QUEST (2014)

These findings are a benchmark for understanding VH repertoires and can be used to characterize the VH repertoire during immune responses. The study will allow standardized comparison for high throughput results obtained by IMGT/HighV-QUEST, the reference portal for NGS repertoire.
Ischemic intestinal complications in patients with burns. (1991)

Enteral bacterial translocation has been implicated as a major cause of morbidity in instances of trauma, but little clinical evidence of the loss of intestinal mucosal integrity has been documented. A retrospective review of the medical and autopsy records of all patients admitted between 1982 and 1988 was performed to describe the incidence of intestinal pathologic findings. Of all deaths, a total of 53 per cent of adults (greater than 18 years old) and 61 per cent of children (zero to 17 years old) were noted to have ischemic intestinal pathologic findings on autopsy, ranging from superficial necrosis to full thickness mucosal necrosis extending into the omentum. More than 80 per cent of these patients were septic at the time of death, with endogenous intestinal flora species most frequently identified as the causative agent. Less than 1 per cent (n = 16) of the patients admitted were clinically identified with intestinal pathologic examination, surgically explored and underwent resection of the intestine. Despite intervention, this group suffered a 69 per cent mortality rate. Although the documentation of bacterial translocation in a human model may be obscured by other potential sources of contamination, for example, burn wound, in combination with the immunosuppression inherent to the thermally injured, may lead to bacterial contamination of the systemic circulation and, therefore, may be a significant contributor to morbidity and mortality after thermal injury.
CFTR activity and mitochondrial function (2013)

Cystic Fibrosis (CF) is a frequent and lethal autosomal recessive disease, caused by mutations in the gene encoding the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Before the discovery of the CFTR gene, several hypotheses attempted to explain the etiology of this disease, including the possible role of a chloride channel, diverse alterations in mitochondrial functions, the overexpression of the lysosomal enzyme α-glucosidase and a deficiency in the cytosolic enzyme glucose 6-phosphate dehydrogenase. Because of the diverse mitochondrial changes found, some authors proposed that the affected gene should codify for a mitochondrial protein. Later, the CFTR cloning and the demonstration of its chloride channel activity turned the mitochondrial, lysosomal and cytosolic hypotheses obsolete. However, in recent years, using new approaches, several investigators reported similar or new alterations of mitochondrial functions in Cystic Fibrosis, thus rediscovering a possible role of mitochondria in this disease. Here, we review these CFTR-driven mitochondrial defects, including differential gene expression, alterations in oxidative phosphorylation, calcium homeostasis, oxidative stress, apoptosis and innate immune response, which might explain some characteristics of the complex CF phenotype and reveals potential new targets for therapy.
Hormonal regulation of a cysteine proteinase gene, EPB‐1, in barley aleurone layers: cis‐ and trans‐acting elements involved in the co‐ordinated gene expression regulated by gibberellins and abscisic acid (1999)

The synthesis of EPB, a cysteine proteinase responsible for the degradation of seed endosperm storage proteins in barley (Hordeum vulgare), is induced by gibberellins (GA) and repressed by abscisic acid (ABA). The EPB gene family consists of two very similar members, EPB-1 and EPB-2, with the former being more highly induced by GA. We have functionally characterized the cis-acting elements in the EPB-1 promoter and determined that a gibberellin response element (GARE), a pyrimidine box and an upstream element are necessary for GA induction. By comparison with the promoters of alpha-amylase genes, which are also induced by GA, we suggest that GARE is coupled with the upstream element and the pyrimidine box to form a GA response complex. In addition, we have shown that the 3'-untranslated/untranscribed region of the EPB-1 gene is required for a low background expression in the absence of GA. Constitutive expression of a transcription factor, GAMyb, in the absence of GA leads to the transactivation of EPB-1 expression in a dosage dependent manner with the highest level comparable to that in fully GA-induced tissue. Co-expression of a truncated version of GAMyb containing only the DNA binding domain blocks the GA-induction of EPB-1, further supporting the role of GAMyb in the regulation of gene expression. Although ABA is very effective in blocking the GA induction of EPB-1, it has no effect on the GAMyb-mediated expression of EPB-1. We suggest that ABA acts upstream of the formation of functional GAMyb which co-ordinates the hormonal regulation of a diverse group of genes in cereal aleurone layers, including those encoding EPB and alpha-amylases.
Development of a novel therapy for Lipo‐oligosaccharide‐induced experimental neuritis: use of peptide glycomimics (2010)

Recent etiological studies have revealed that molecular mimicry between the lipo-oligosaccharide (LOS) component of Campylobacter jejuni and gangliosides of peripheral nervous system plays an important role in the pathogenesis of Guillain-Barré syndrome (GBS). Previously, we demonstrated GD3 ganglioside molecular mimicry in a model of GBS in Lewis rats by sensitization with GD3-like LOS (LOS(GD3)) from C. jejuni. Since the neuropathophysiological consequences were due largely to the anti-GD3-like antibodies, we subsequently focused our effort upon eliminating the pathogenic antibodies using several strategies to mimic GD3 in this model. Here, we have validated this strategy by the use of peptide glycomimics based on epitopic mimicry between carbohydrates and peptides. We treated rats by i.p. administration of phage-displayed GD3-like peptides. One GD3-like peptide (P(GD3)-4; RHAYRSMAEWGFLYS) induced in treated rats a remarkable restoration of motor nerve functions, as evidenced by improved histopathology, rotarod performance, and motor nerve conduction velocity. P(GD3)-4 effectively decreased the titer of anti-GD3/anti-LOS(GD3) antibodies and ameliorated peripheral nerve dysfunction in the sera of treated rats. The data suggest that peptide glycomimics of ganglioside may be potential powerful reagents for therapeutic intervention in GBS by neutralizing specific pathogenic anti-ganglioside antibodies.
Novel Chiral Auxiliaries of BIAZOLs (2001)

Asymmetric catalysis is one of the most challenging and formidable endeavor in organic synthesis. The development of chiral auxiliaries is a key in the asymmetric catalysis. Azulenoids, a parent structure of bicyclo[5.3.0]decapentaene with 10 πelectrons, are useful as dye materials, medical treatment of inflammation and hypertension, and the development of liquid crystals. In continuing to investigate synthetic application of azulenoids, we have studied to develop novel chiral auxiliaries of BIAZOLs. The BIAZOLs were synthesized from dicyclopentadiene and characterized using spectroscopies.
Diesters from Oleic Acid: Synthesis, Low Temperature Properties, and Oxidation Stability (2007)

Abstract Several diesters were prepared from commercially available oleic acid and common organic acids. The key step in the three step synthesis of oleochemical diesters entails a ring opening esterification of alkyl 9,10‐epoxyoctadecanoates (alkyl: propyl, isopropyl, octyl, 2‐ethylhexyl) using propionic and octanoic acids without the need for either solvent or catalyst. Each synthetic diester was evaluated for both low temperature operability and oxidation stability through measurement of cloud point, pour point, oxidation onset temperature, and signal maximum temperature. It was discovered that increasing chain length of the mid‐chain ester and branching in the end‐chain ester had a positive influence on the low temperature properties of diesters. Improved oxidation stability is achieved when the chain length of the mid‐chain ester is decreased. Additionally, the mid‐chain ester plays a larger role in oxidation stability than the end‐chain ester. These products may prove useful in the search for bio‐based industrial materials, such as lubricants, surfactants, and fuel additives.