gebhart/patrepeval huggingface dataset:
Aberrant expression of the growth factor Wnt-5A in human malignancy. (1995)

The Wnt-5A gene codes for a secreted cysteine-rich growth factor that mediates cell to cell signaling via a paracrine mechanism during development and ontogeny. We have recently determine the genomic organization and chromosomal mapping of the human Wnt-5A, and observed distinct patterns of expression in developing human embryos. In this report, we have performed a detailed expression analysis of 100 adult human tissues and tumors and 10 human cell lines. Our data show a widespread expression of Wnt-5A in adult tissues and cells, and aberrant mRNA levels in lungs, breast, and prostate carcinomas and in melanomas. The up-regulation of Wnt-5A in human malignancy was not due to either gene rearrangement or amplification. These findings document an abnormal expression of this growth factor in malignancy and implicate Wnt-5A in the genesis of human cancer.
Interaction between Particles in a Nematic Liquid Crystal: Numerical Study Using the Landau-de Gennes Continuum Theory (2005)

ABSTRACT We study numerically the interaction between particles in a nematic liquid crystal mediated by its elastic distortions with the aid of the Landau-de Gennes continuum theory. We consider the cases where two particles impose rigid normal anchoring on their surfaces and are accompanied by a hyperbolic hedgehog defect. As a function of the distance between the centers of the particles D, we evaluate the force f acting on the particles by integrating the stress tensor. The result is well described by a power law f ∝ D −x . When the “dipoles”, composed of a particle and a hyperbolic hedgehog, are in parallel directions, the interaction is attractive and the exponent is x ≃ 4, consistent with the experimental observations together with the theoretical expectation of the dipole-dipole interaction. For antiparallel dipoles, repulsive interaction is observed and x ≃ 3.6, slightly stronger than the dipole-dipole interaction.
Function of JunB in Transient Amplifying Cell Senescence and Progression of Human Prostate Cancer (2008)

JunB is an essential upstream regulator of p16 and contributes to maintain cell senescence that blocks malignant transformation of TAC. JunB thus apparently plays an important role in controlling prostate carcinogenesis and may be a new target for cancer prevention and therapy.
Further studies in man of hsw1n1 influenza viruses (1980)

Two subpopulations of antigenically different Hsw1N1 influenza viruses, cloned from 'swine' New Jersey virus 1976, were individually inoculated into antibody-free volunteers. One clone contained a haemagglutinin so far seen only in swine viruses prevalent in 1971 and after, the other a haemagglutinin of earlier strains dating back to a least 1957. Each of the viruses was infectious for man and intermediate in human virulence between a wild human virus and swine pathogens of 1966 and 1967, which had earlier been tested in man. Antigenically comparable clones segregated from viruses recovered in Wisconsin from a pig and a human contact, respectively, were also infectious for man; however, they were less virulent than their New Jersey counterparts. Differences between the Wisconsin clones themselves were small, but there was an indication of a relationship between human passage. and human virulence. There was no evidence of inherently greater human virulence in the newer Hsw1N1 serotype as compared with the earlier serotype. Hence, recent detection of swine influenza-like viruses in man is unlikely to be the consequence of a host-range mutation concurrent with an antigenic mutation. We believe that the Hsw1N1 viruses recovered from the human influenza outbreak at Fort Dix, New Jersey, and from recent single human infections were wholly derived from enzootic swine viruses that underwent limited human adaptation through man-to-man passage.
Construction and characterization of an Escherichia coli strain with a uncI mutation (1984)

A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombinant plasmid containing the mutation, in a temperature-sensitive polA strain. The mutant strain is phenotypically unc+ but has a reduced growth yield compared to a normal sibling strain.
Flip-Chip Bonding Using Superconducting Solder Bump (1995)

Flip-chip bonding using superconducting solder bumps consists of two technologies: acid-free thermosonic compression ball bonding and face-down bonding of the chip on the substrate by means of thermosonic compression at 373 K. A superconducting test chip with 68 bumps formed on Nb/Pd film pads was bonded on a ceramic substrate. The maximum shear strength of a single solder bump for the Nb/Pd films was found to be 26.5 MPa, which was about 3 times higher than that obtained for Nb/Au film pads. The flip-chip module did not undergo catastrophic failures in either prototype design or material combination during 20 thermal cyclings between 4.2 K and 273 K. Electrical check tests at 4.2 K before and after the thermal cyclings suggest that the present superconducting solder bump can be put into practical use for superconducting IC chip connection.
Varicella Vaccine Studies in Healthy Children and Adults (1986)

Immunization of normal children and adults with Oka strain live varicella vaccine from several manufacturers has been studied in our laboratory and elsewhere. This paper summarizes clinical trials designed to obtain information on minimum dose immunogenicity pre- and postexposure prophylaxis, immunization of various age groups, and booster immunizations for seropositive individuals. These studies documented a 94% to 100% seroconversion rate with 95% to 100% persistence of antibodies at 3 to 4 years. Protective efficacy was more than 90%, and the vaccine was successful in preventing varicella when a high dose was given postexposure. Clinical reactions were limited to temperature elevations and minor papulovesicular rashes that occurred in 5% to 10% of vaccinees. Herpes zoster has been absent in vaccinated healthy individuals.
Imaging and Analysis of Immobilized Particle Arrays (2003)

An automated imaging system was developed to quantify fluorescence signals from particles immobilized on hydrogel-coated slides. Arrays of submicrometer-diameter particles were printed with up to 600 particles/spot. The slides were read under 20x magnification without cover slips. Software was written to image individual spots and measure the median particle fluorescence in each spot. To locate array spots, an alignment program made use of two fiducial grids of fluorescent reference particles at either end of the slide. Focusing was adjusted locally using spots of reference particles located at the centers of focusing neighborhoods. The response was linear across a two-decade range, and the precision of readings was better than 5% down to approximately 1000 fluors/particle. Exposure times varied with signal intensity, reaching 1 s at the lowest levels of fluorescence. Data demonstrate feasibility for measuring fluorescence from immobilized particle arrays on an automated microscope with accuracy and precision similar to fluorescence measurements of microparticles with a flow cytometer. This work provides automation of imaging and analysis procedures necessary for development of immobilized particle arrays as an analytical platform that combines advantageous features of both planar and suspension arrays.
Expression of GATA-binding proteins during embryonic development in Xenopus laevis. (1991)

Proteins that recognize the core sequence GATA are important regulators of hematopoietic-specific gene transcription. We have characterized cDNAs encoding the Xenopus laevis homologues of three related transcription factors, designated GATA-1, -2, and -3. Comparative sequence analysis reveals strong conservation of the zinc-finger DNA-binding domain among all vertebrate GATA-binding proteins. GATA-2 and GATA-3 polypeptides are homologous throughout their entire sequences, whereas GATA-1 sequence is conserved only in the region responsible for DNA binding. In Xenopus, RNAs encoding GATA-binding proteins are expressed in both larval and adult erythroid cells. GATA-1, -2, and -3 RNAs are first detectable in early gastrula (Nieuwkoop developmental stage 11). This is earlier than the appearance of the early larval alpha T1 globin RNA (stage 15), beta T1 globin RNA (stage 26), or blood island formation (stage 30). The expression of GATA-1, -2, and -3 in early development may signal an early commitment of mesoderm to form hematopoietic tissue.
A quasi-passive model of human leg function in level-ground walking (2006)

In this paper, we seek to understand how leg muscles and tendons work mechanically during walking in order to motivate the design of efficient robotic legs. We hypothesize that a robotic leg comprising only knee and ankle passive and quasi-passive elements, including springs, clutches and variable-damping components, can capture the dominant mechanical behavior of the human knee and ankle during level-ground walking at self-selected speeds. As a preliminary evaluation of this hypothesis, we put forth a simple leg model that captures the gross features of the human leg musculoskeletal architecture. We vary model parameters, or spring constants, damping levels and times when clutches are engaged, using an optimization scheme where errors between model joint behaviours and biological joint mechanics are minimized. For model evaluation, kinetic and kinematic gait data are employed from a single participant walking across a level-ground surface at a self-selected gait speed (1.3 m/sec). With only a single hip actuator, we find good agreement between model predictions and experimental gait data, suggesting that knee and ankle actuators are not necessary for level-ground robotic ambulation at self-selected gait speeds. This result is in support of the idea that muscles that span the human knee and ankle mainly operate eccentrically or isometrically, affording the relatively high metabolic walking economy of humans
Replication-Competent Herpes Simplex Virus Vector G207 and Cisplatin Combination Therapy for Head and Neck Squamous Cell Carcinoma (1999)

Replication-competent virus vectors are attractive therapeutic agents for cancer. G207, a second-generation, multimutated herpes simplex virus type 1 (HSV-1), is one such vector that is safe in primates and efficacious against human tumors in athymic mice. Squamous cell carcinoma is the most frequently encountered malignancy of the head and neck, and the chemotherapeutic agent cisplatin is a standard treatment for recurrent head and neck cancer. In this study we examine the therapeutic potential of G207, alone and in combination with cisplatin, against squamous cell carcinoma. Human squamous cell carcinoma cell lines are sensitive to G207 replication and cytotoxicity in vitro at a multiplicity of infection of 0.01, including cisplatin sensitive (UMSCC-22A), moderately sensitive (UMSCC-38), and weakly sensitive (SQ20B) cell lines. Cisplatin did not inhibit the cytopathic effect of G207. G207 inhibited the growth of established subcutaneous head and neck tumors in athymic mice. The therapeutic effects of cisplatin and G207 in vivo were independent. However, in cisplatin-sensitive tumors (UMSCC-38), combination therapy resulted in 100% cures in contrast to 42% with G207 or 14% with cisplatin alone. We conclude that G207 should be considered for the treatment of head and neck cancer and that combination with chemotherapeutic agents may improve efficacy.
Time-Lapse Crosswell Seismic Tomography for Monitoring Injected CO2 in an Onshore Aquifer, Nagaoka, Japan (2006)

AbstractJapan's first pilot-scale CO2 sequestration experiment has been conducted in Nagaoka, where 10 400 t of CO2 have been injected in an onshore aquifer at a depth of about 1100 m. Among various measurements conducted at the site for monitoring the injected CO2, we conducted time-lapse crosswell seismic tomography between two observation wells to determine the distribution of CO2 in the aquifer by the change of P-wave velocities. This paper reports the results of the crosswell seismic tomography conducted at the site.The crosswell seismic tomography measurements were carried out three times; once before the injection as a baseline survey, and twice during the injection as monitoring surveys. The velocity tomograms resulting from the monitoring surveys were compared to the baseline survey tomogram, and velocity difference tomograms were generated. The velocity difference tomograms showed that velocity had decreased in a part of the aquifer around the injection well, where the injected CO2 was supposed to be distributed. We also found that the area in which velocity had decreased was expanding in the formation up-dip direction, as increasing amounts of CO2 were injected. The maximum velocity reductions observed were 3.0% after 3200 t of CO2 had been injected, and 3.5% after injection of 6200 t of CO2.Although seismic tomography could map the area of velocity decrease due to CO2 injection, we observed some contradictions with the results of time-lapse sonic logging, and with the geological condition of the cap rock. To investigate these contradictions, we conducted numerical experiments simulating the test site. As a result, we found that part of the velocity distribution displayed in the tomograms was affected by artefacts or ghosts caused by the source-receiver geometry for the crosswell tomography in this particular site. The maximum velocity decrease obtained by tomography (3.5%) was much smaller than that observed by sonic logging (more than 20%). The numerical experiment results showed that only 5.5% velocity reduction might be observed, although the model was given a 20% velocity reduction zone. Judging from this result, the actual velocity reduction can be more than 3.5%, the value we obtained from the field data reconstruction. Further studies are needed to obtain more accurate velocity values that are comparable to those obtained by sonic logging.time-lapsecrosswellseismic tomographyCO2 injectiononshore aquiferNagaoka
On the different response of Salmonella typhimurium hisG46 and TA1530 to mutagenic action of base analogues. (1979)

Various mutagens are known to induce more his+ revertants in TA1530 than in hisG46 strain. To test whether the mutator effect shown by TA1530 is limited to the his mutation, the lysA8 marker was introduced into both the TA1530 and hisG46 strain, and its reversibility, after induction by N4-hydroxycytidine, was estimated. The ability to reverse the lys marker was tenfold higher in the TA1530/lysA8 transductants than in the hisG46/lysA8 transductants or in the donor for lys, the lysA8 strain.
The N-Terminal Extension of the Influenza B Virus Nucleoprotein Is Not Required for Nuclear Accumulation or the Expression and Replication of a Model RNA (1998)

The nucleoprotein (NP) of influenza B virus is 50 amino acids longer at the N-terminus than influenza A virus NP and lacks homology to the A virus protein over the first 69 residues. We have deleted the N-terminal 51 and 69 residues of the influenza B/Ann Arbor/1/66 virus NP and show that nuclear accumulation of the protein is unaffected. This indicates that the nuclear localization signal is not located at the extreme N terminus, as in influenza A virus NP. To determine if the N-terminal mutants could support the expression and replication of a model influenza B virus RNA, the genes encoding the subunits of the viral RNA-dependent RNA polymerase (PA, PB1, and PB2) were cloned. Coexpression of NP and the P proteins in 293 cells was found to permit the expression and replication of a transfected model RNA based on segment 4 of B/Maryland/59, in which the hemagglutinin-coding region was replaced by a chloramphenicol acetyltransferase gene. The expression and replication of the synthetic RNA were not affected by the replacement of NP with NP mutants lacking the N-terminal 51 or 69 residues, indicating that the N-terminal extension is not required for transcription or replication of the viral RNA. In addition, we report that the influenza B virus NP cannot be functionally replaced by type A virus NP in this system.
Therapeutic Angiogenesis in Critical Limb and Myocardial Ischemia (2001)

Research in animal models of ischemia has shown that administration of angiogenic growth factors, either as a recombinant protein or by gene transfer, can augment nutrient perfusion through neovascularization to promote the development of supplemental collateral blood vessels that will constitute endogenous bypass conduits around occluded native arteries; a strategy termed "therapeutic angiogenesis." In animal models and clinical trials, the best studied cytokines with angiogenic activity are vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). Clinical trials of therapeutic angiogenesis in patients with critical limb ischemia demonstrated resolution of rest pain and/or improved limb integrity, increased pain-free walking time and ankle-brachial index, newly visible collateral vessels by digital subtraction angiography, and qualitative evidence of improved distal flow by magnetic resonance imaging. Initial clinical trials in patients with end-stage coronary artery disease using direct myocardial injection via thoracotomy resulted in large increases in exercise time and marked reductions in anginal symptoms, as well as objective evidence of improved perfusion and left ventricular function. Larger scale placebo-controlled trials have been limited to intracoronary and intravenous administration of recombinant protein, and have not shown significant improvement in exercise time or angina compared to placebo. Larger scale placebo-controlled studies of gene transfer using catheter-based endocardial delivery are in progress. Future clinical studies are required to determine the optimal dose, formulation, route of administration, and combinations of growth factors, as well as the requirement for endothelial progenitor cell or stem cell supplementation, to provide effective and safe therapeutic angiogenesis for patients with critical limb ischemia and chronic myocardial ischemia who are not candidates for conventional revascularization procedures.
In Vitro Generation of Functional Liver Organoid-Like Structures Using Adult Human Cells (2015)

In this study we used differentiated adult human upcyte® cells for the in vitro generation of liver organoids. Upcyte® cells are genetically engineered cell strains derived from primary human cells by lenti-viral transduction of genes or gene combinations inducing transient proliferation capacity (upcyte® process). Proliferating upcyte® cells undergo a finite number of cell divisions, i.e., 20 to 40 population doublings, but upon withdrawal of proliferation stimulating factors, they regain most of the cell specific characteristics of primary cells. When a defined mixture of differentiated human upcyte® cells (hepatocytes, liver sinusoidal endothelial cells (LSECs) and mesenchymal stem cells (MSCs)) was cultured in vitro on a thick layer of Matrigel™, they self-organized to form liver organoid-like structures within 24 hours. When further cultured for 10 days in a bioreactor, these liver organoids show typical functional characteristics of liver parenchyma including activity of cytochromes P450, CYP3A4, CYP2B6 and CYP2C9 as well as mRNA expression of several marker genes and other enzymes. In summary, we hereby describe that 3D functional hepatic structures composed of primary human cell strains can be generated in vitro. They can be cultured for a prolonged period of time and are potentially useful ex vivo models to study liver functions.
1H nuclear magnetic resonance of human seminal plasma in in vitro fertilization attempts: use of automatic spectrum analysis (1995)

Seminal plasma affects the quality of spermatozoa. The possible use of 1H nuclear magnetic resonance (NMR) analysis of human seminal plasma to determine a full biochemical profile that would allow the prediction of successful in vitro fertilization was examined. Two seminal plasma patterns (GI and GII) were found. The main compounds in GI were glycerophosphorylcholine, citrate and lactate, and in GII, choline and amino acids. These patterns did not fit into the usual spermogram classes. However, GI exhibited the higher pregnancy rate, suggesting that the equilibrium established between the components in seminal plasma affected not only the quality of spermatozoa but also embryonic quality.
STIMULATION OF THE VAGUS NERVE AND CAROTID SINUS IN MAN (1932)

The results of mechanical stimulation of the exposed vagus nerve and carotid sinus of man in a series of twenty cases have been recorded electrocardiographically. Of thirteen cases under ether anæsthesia, none showed any response to sinus stimulation, while one responded to vagal stimulation. Two cases under chloroform yielded responses to sinus stimulation, and one to vagal stimulation also. Of five cases under gas and oxygen, three gave responses to sinus or vagal stimulation, or to both. The response to sinus stimulation was usually moderate bradycardia, with or without diminution of the P‐waves. A different response was elicited under chloroform, consisting of the appearance of ventricular extrasystoles. Responses to vagal stimulation were similar in nature, but were characterised by a longer latent period and longer after‐effect. It has been demonstrated that the human vagus is in some cases excitable by mechanical means. The relation of this to Czermak's “vagal pressure” and to the “caroto‐vagal” pressure of Hering is discussed. The expenses of this research have been met by grants from the Moray Endowment Fund and from the Trustees of the Clinical Medicine Research Laboratory, Edinburgh Royal Infirmary.
User interface for a digital library to support construction of a "virtual personal library" (2002)

Describes the development of a prototype user interface to support the construction of "virtual personal libraries" from digital libraries. The virtual personal library provides broad support for user-information activities such as browsing, editing, storing and annotating. We propose the use of a book metaphor interface that represents virtual books and bookshelves on computer displays. Users can browse, edit and store information freely by using this interface. We also propose a pointer-storing method based on the concept of transclusion to legally validate the use of information resources. An extended HTML is used to realize the pointer-storing method on the World Wide Web.