gebhart/patrepeval huggingface dataset:
PPAR-γ Ligands Repress TGFβ-Induced Myofibroblast Differentiation by Targeting the PI3K/Akt Pathway: Implications for Therapy of Fibrosis (2011)

Transforming growth factor beta (TGFβ) induced differentiation of human lung fibroblasts to myofibroblasts is a key event in the pathogenesis of pulmonary fibrosis. Although the typical TGFβ signaling pathway involves the Smad family of transcription factors, we have previously reported that peroxisome proliferator-activated receptor-γ (PPAR-γ) ligands inhibit TGFβ-mediated differentiation of human lung fibroblasts to myofibroblasts via a Smad-independent pathway. TGFβ also activates the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway leading to phosphorylation of Akt(S473). Here, we report that PPAR-γ ligands, 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid (CDDO) and 15-deoxy-(12,14)-15d-prostaglandin J(2) (15d-PGJ(2)), inhibit human myofibroblast differentiation of normal and idiopathic pulmonary fibrotic (IPF) fibroblasts, by blocking Akt phosphorylation at Ser473 by a PPAR-γ-independent mechanism. The PI3K inhibitor LY294002 and a dominant-negative inactive kinase-domain mutant of Akt both inhibited TGFβ-stimulated myofibroblast differentiation, as determined by Western blotting for α-smooth muscle actin and calponin. Prostaglandin A(1) (PGA(1)), a structural analogue of 15d-PGJ(2) with an electrophilic center, also reduced TGFβ-driven phosphorylation of Akt, while CAY10410, another analogue that lacks an electrophilic center, did not; implying that the activity of 15d-PGJ(2) and CDDO is dependent on their electrophilic properties. PPAR-γ ligands inhibited TGFβ-induced Akt phosphorylation via both post-translational and post-transcriptional mechanisms. This inhibition is independent of MAPK-p38 and PTEN but is dependent on TGFβ-induced phosphorylation of FAK, a kinase that acts upstream of Akt. Thus, PPAR-γ ligands inhibit TGFβ signaling by affecting two pro-survival pathways that culminate in myofibroblast differentiation. Further studies of PPAR-γ ligands and small electrophilic molecules may lead to a new generation of anti-fibrotic therapeutics.
Military and civilian burn injuries during armed conflicts. (2007)

Burn injury is a ubiquitous threat in the military environment, and war burns have been described for more than 5,000 years of written history. Fire was probably utilized as a weapon long before that. With the ever-increasing destructive power and efficiency of modern weapons, casualties, both fatal and non-fatal, are reaching new highs, particularly among civilians who are becoming the major wartime targets in recent wars, accounting for most of the killed and wounded. Even though medical personnel usually believe that a knowledge of weaponry has little relevance to their ability to effectively treat injuries and that it may in some way be in conflict with their status, accorded under the Geneva and Hague treaties, it is imperative that they know how weapons are used and understand their effects on the human body. The present review explores various categories of weapons of modern warfare that are unfamiliar to most medical and paramedical personnel responsible for burn treatment. The mechanisms and patterns of injury produced by each class of weapons are examined so that a better understanding of burn management in a warfare situation may be achieved.
Neuropsychology and emotion processing in violent individuals with antisocial personality disorder or schizophrenia: The same or different? A systematic review and meta-analysis (2017)

There are qualitatively similar, but quantitatively different, neuropsychological and emotion processing deficits in violent individuals with schizophrenia and antisocial personality disorder which could be developed into transdiagnostic treatment targets for violent behaviour. Future research should aim to characterise specific subgroups of violent offenders, including those with comorbid diagnoses.
QUASI-ELASTIC LIGHT SCATTERING APPLICATIONS IN BIOCHEMISTRY AND BIOLOGY (1981)

Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific ...Read More
cDNA cloning of the B cell membrane protein CD22: a mediator of B-B cell interactions. (1991)

We have cloned a full-length cDNA for the B cell membrane protein CD22, which is referred to as B lymphocyte cell adhesion molecule (BL-CAM). Using subtractive hybridization techniques, several B lymphocyte-specific cDNAs were isolated. Northern blot analysis with one of the clones, clone 66, revealed expression in normal activated B cells and a variety of B cell lines, but not in normal activated T cells, T cell lines, Hela cells, or several tissues, including brain and placenta. One major transcript of approximately 3.3 kb was found in B cells although several smaller transcripts were also present in low amounts (approximately 2.6, 2.3, and 1.6 kb). Sequence analysis of a full-length cDNA clone revealed an open reading frame of 2,541 bases coding for a predicted protein of 847 amino acids with a molecular mass of 95 kD. The BL-CAM cDNA is nearly identical to a recently isolated cDNA clone for CD22, with the exception of an additional 531 bases in the coding region of BL-CAM. BL-CAM has a predicted transmembrane spanning region and a 140-amino acid intracytoplasmic domain. Search of the National Biological Research Foundation protein database revealed that this protein is a member of the immunoglobulin super family and that it had significant homology with three homotypic cell adhesion proteins: carcinoembryonic antigen (29% identity over 460 amino acids), myelin-associated glycoprotein (27% identity over 425 amino acids), and neural cell adhesion molecule (21.5% over 274 amino acids). Northern blot analysis revealed low-level BL-CAM mRNA expression in unactivated tonsillar B cells, which was rapidly increased after B cell activation with Staphylococcus aureus Cowan strain 1 and phorbol myristate acetate, but not by various cytokines, including interleukin 4 (IL-4), IL-6, and gamma interferon. In situ hybridization with an antisense BL-CAM RNA probe revealed expression in B cell-rich areas in tonsil and lymph node, although the most striking hybridization was in the germinal centers. COS cells transfected with a BL-CAM expression vector were immunofluorescently stained positively with two different CD22 antibodies, each of which recognizes a different epitope. Additionally, both normal tonsil B cells and a B cell line were found to adhere to COS transfected with BL-CAM in the sense but not the antisense direction.(ABSTRACT TRUNCATED AT 400 WORDS)
Cloning and characterization of a bifunctional metabotropic receptor activated by both extracellular calcium and glutamate (1996)

A cDNA from salmon brain which induces a consistent activation of Ca2+ dependent Cl- (Ca(2+)-Cl-) current in Xenopus oocytes in the saline solution was isolated by an expression cloning method. The primary structure showed a high homology with the metabotropic glutamate receptors (mGluR), and a limited homology with the Ca2+/polyvalent cation receptors (CaR). The encoded protein was functionally characterized for the sensitivity to extracellular Ca2+ (Cao2+), and the consistent activation of Ca(2+)-Cl- current was determined to be due to Ca2+ contained in the saline solution. It was also confirmed that the encoded protein has a function to sense glutamate. An increase in inositol 1,4,5-triphosphate turnover was observed when either Ca2+ or glutamate was applied. Thus, the encoded protein was identified as a bifunctional metabotropic receptor for both Ca2+ and glutamate. In addition, rat mGluR1 alpha was also shown to be activated by Cao2+. This is the first report of a G-protein coupled receptor which is activated by two totally different physiological agonists.
Cardiac muscarinic receptors decrease with age. In vitro and in vivo studies. (1998)

The M1 muscarinic receptor antagonist pirenzepine in low doses decreases resting heart rate; this effect declines with age (Poller, U., G. Nedelka, J. Radke, K. Pönicke, and O.-E. Brodde. 1997. J. Am. Coll. Cardiol. 29:187-193). To study possible mechanisms underlying this effect, we assessed (a) in six young (26 yr old) and six older volunteers (61 yr old), pirenzepine effects (0.32 and 0.64 mg intravenous [i.v.] bolus) on isoprenaline-induced heart rate increases; (b) in five heart transplant recipients, pirenzepine effects (0.05-10 mg i.v. bolus) on resting heart rate in the recipient's native and transplanted sinus nodes; and (c) in right atria from 39 patients of different ages (5 d-76 yr) undergoing open heart surgery, M2 muscarinic receptor density (by [3H]N-methyl-scopolamine binding) and adenylyl cyclase activity. (a) Pirenzepine at both doses decreased heart rate in young volunteers significantly more than in older volunteers; (b) pirenzepine (< 1 mg) decreased resting heart rate in the recipient's native but not transplanted sinus node; and (c) M2 receptor density and carbachol-induced inhibition of forskolin-stimulated adenylyl cyclase activity decreased significantly with the age of the patients. We conclude that pirenzepine decreases heart rate via inhibition of presynaptic M1 autoreceptors, thereby releasing endogenous acetylcholine, and that the heart rate-decreasing effect of acetylcholine declines with age because right atrial M2 receptor density and function decrease.
Advanced acoustic design for a new seismic streamer (1983)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 1983Advanced acoustic design for a new seismic streamerAuthors: J. H. PeacockC. G. SykesN. W. CameronL. G. PeardonJ. H. PeacockBritoil, C. G. SykesStandard Telephones and Cables, N. W. CameronStandard Telephones and Cables, and L. G. PeardonBritoil, Englandhttps://doi.org/10.1190/1.1893760 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1893760FiguresReferencesRelatedDetailsCited ByNoise types and their attenuation in towed marine seismic: A tutorialVolodya Hlebnikov, Thomas Elboth, Vetle Vinje, and Leiv-J. Gelius23 February 2021 | GEOPHYSICS, Vol. 86, No. 2Flow noise reduction from superhydrophobic surfacesGEOPHYSICS, Vol. 77, No. 1Investigation of flow and flow noise around a seismic streamer cableGEOPHYSICS, Vol. 75, No. 1Flow and swell noise in marine seismic dataGEOPHYSICS, Vol. 74, No. 2Attenuation of noise in marine seismic dataThomas Elboth, Fugro Geoteam, and Dag Hermansen14 October 2009 SEG Technical Program Expanded Abstracts 1983ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 1983 Pages: 646 publication data© 1983 Copyright © 1983 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished: 24 Mar 2005 CITATION INFORMATION J. H. Peacock, C. G. Sykes, N. W. Cameron, and L. G. Peardon, (1983), "Advanced acoustic design for a new seismic streamer," SEG Technical Program Expanded Abstracts : 465-466. https://doi.org/10.1190/1.1893760 Plain-Language Summary PDF DownloadLoading ...
Fault analysis based on integration of digital relay and DFR data (2005)

This paper discusses integration of two existing automated analysis applications, DFR data analysis and digital relay data analysis, to achieve comprehensive fault analysis. As inputs to the integrated application, digital relay files and reports are introduced. The proposed strategy and implementation of integration are outlined. An example is used to demonstrate features of the integrated application developed so far.
The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2 (1993)

The adenovirus E1A oncoproteins form stable complexes with several cellular proteins. Association of E1A with these proteins has been shown to be important for the oncogenic potential of E1A. Several of these proteins have been identified and include the product of the retinoblastoma gene and a key cell cycle regulatory protein, cyclin A. E1A also associates with a potent histone H1 kinase. The two major components of this activity are the cyclin E-p33cdk2 and cyclin A-p33cdk2 complexes. Both the cyclin E-p33cdk2 and cyclin A-p33cdk2 complexes have been implicated in regulatory events controlling entry into or passage through DNA synthesis. Although the architecture of such interactions remains unclear, it is likely that by targeting such complexes, adenovirus is affecting some aspect of cell cycle control.
On Distributed Communications Networks (1964)

This paper briefly reviews the distributed communication network concept in which each station is connected to all adjacent stations rather than to a few switching points, as in a centralized system. The payoff for a distributed configuration in terms of survivability in the cases of enemy attack directed against nodes, links or combinations of nodes and links is demonstrated. A comparison is made between diversity of assignment and perfect switching in distributed networks, and the feasibility of using low-cost unreliable communication links, even links so unreliable as to be unusable in present type networks, to form highly reliable networks is discussed. The requirements for a future all-digital data distributed network which provides common user service for a wide range of users having different requirements is considered. The use of a standard format message block permits building relatively simple switching mechanisms using an adaptive store-and-forward routing policy to handle all forms of digital data including digital voice. This network rapidly responds to changes in the network status. Recent history of measured network traffic is used to modify path selection. Simulation results are shown to indicate that highly efficient routing can be performed by local control without the necessity for any central, and therefore vulnerable, control point.
Improving relevance judgment of web search results with image excerpts (2008)

Current web search engines return result pages containing mostly text summary even though the matched web pages may contain informative pictures. A text excerpt (i.e. snippet) is generated by selecting keywords around the matched query terms for each returned page to provide context for user's relevance judgment. However, in many scenarios, we found that the pictures in web pages, if selected properly, could be added into search result pages and provide richer contextual description because a picture is worth a thousand words. Such new summary is named as image excerpts. By well designed user study, we demonstrate image excerpts can help users make much quicker relevance judgment of search results for a wide range of query types. To implement this idea, we propose a practicable approach to automatically generate image excerpts in the result pages by considering the dominance of each picture in each web page and the relevance of the picture to the query. We also outline an efficient way to incorporate image excerpts in web search engines. Web search engines can adopt our approach by slightly modifying their index and inserting a few low cost operations in their workflow. Our experiments on a large web dataset indicate the performance of the proposed approach is very promising.
T-Cell Subsets (1982)

To the Editor.— I'm OKT3, you're OKT4... Dr Goodwin surely jests in his diatribe against the T-cell subsets as he bemoans the volume and triviality of these determinations. His complaints seem similar to those bandied about regularly when a new procedure points to new understanding of metabolic processes, requiring an intellectual adjustment. As for the plethora of articles on the subject of T cells, every new insight into body function leads to an initial accumulation of data to answer the basic three questions: What? When? Where? After these are tabulated the crucial questions How? and Why? can be addressed. Medical residents, who obviously make Dr Goodwin uneasy, are still close enough to the learning process to be both flexible in applying new techniques and curious in continuing to ask why. Identification of T-cell function may be closer to the reality of medicine than even Dr Goodwin's preferred creatinine clearance testing
In vitro propagated dendritic cells from prostate cancer patients as a component of prostate cancer immunotherapy (1995)

T cell-mediated cancer immunotherapy requires efficient antigen-presenting cells. Dendritic cells (DCs) are arguably the most efficient antigen-presenting cells studied to date. Individuals with prostate cancer often undergo various therapies which may compromise their immune system, including the state of their DC precursors. We report the in vitro propagation of DCs from peripheral blood of patients with prostate cancer, most of whom are in clinical stages D1 or D2 and have undergone radiation therapy. After 7 days in culture, the number of DCs recovered were 20-50-fold higher than those isolated directly from peripheral blood. This number is comparable to findings of previous studies with healthy individuals. Cultured patients' DCs were capable of presenting tetanus toxoid to autologous T cells in vitro. Furthermore, T cells from 2 of 4 patients proliferated when cultured with their DCs and the lysate of a human prostate cancer cell line (LNCaP), demonstrating the potential role of autologous DCs in prostate cancer immunotherapy studies.
The visual instruction set (VIS) in UltraSPARC (2002)

This paper describes the visual instruction set (VIS). This is a RISC-like extension to the SPARC V9 instruction set that provides core instructions that greatly enhance the graphics and image processing capabilities of SPARC processors. VIS's first implementation is in the new UltraSPARC microprocessor.
Improved engraftment of human hematopoietic cells in severe combined immunodeficient (SCID) mice carrying human cytokine transgenes. (1995)

We have generated immunodeficient scid-/scid- (SCID)-transgenic mice expressing the genes for human interleukin 3, granulocyte/macrophage-colony stimulating factor, and stem cell factor. We have compared engraftment and differentiation of human hematopoietic cells in transgenic SCID mice with two strains of nontransgenic SCID mice. Human bone marrow cells carrying the CD34 antigen or human umbilical cord blood were injected into sublethally irradiated recipients. Human DNA was detected by polymerase chain reaction in peripheral blood and bone marrow of 14 of 28 transgenic SCID mice after transplantation, but in only 2 of 15 nontransgenic SCID littermates at a 10-fold lower level. Bone marrow cultures 8 wk after transplantation of cord blood gave rise to human burst-forming unit erythroid, colony-forming unit granulocyte/macrophage, or granulocyte/erythroid/macrophage/megakaryocyte colonies. Engraftment was observed for up to 6 mo in transgenic SCID mice, twice as long as nontransgenic littermates or previous studies in which transplanted SCID mice were given daily injections of growth factors. We conclude that the level and duration of engraftment of human cells in SCID mice can be improved by expression of human cytokine transgenes and that transgenic SCID mice are an efficient model system for the study of human hematopoiesis.
Integration of Adeno-Associated Virus (AAV) and Recombinant AAV Vectors (2004)

The driving interest in adeno-associated virus (AAV) has been its potential as a gene delivery vector. The early observation that AAV can establish a latent infection by integrating into the host chromosome has been central to this interest. However, chromosomal integration is a two-edged sword, imparting on one hand the ability to maintain the therapeutic gene in progeny cells, and on the other hand, the risk of mutations that are deleterious to the host. A clearer understanding of the mechanism and efficiency of AAV integration, in terms of contributing viral and host-cell factors and circumstances, will provide a context in which to evaluate these potential benefits and risks. Research to date suggests that AAV integration in any context is inefficient, and that the persistence of AAV gene delivery vectors in tissues is largely attributable to episomal genomes.
Tear glucose levels in normal people and in diabetic patients. (1980)

Fifty normal people and 50 diabetic patients were studied by means of an enzymatic method for the glucose content in the blood, tears, and urine before and after the peroral glucose load. In normal subjects the fasting glucose levels in the tears averaged 3.6 mg/100 ml (0.2 mmol/l) and in the diabetic patients it averaged 16.6 mg/100 ml (0.92 mmol/l). As in blood and urine, tear glucose levels are significantly higher in diabetic patients than normal persons both at fasting and after the peroral glucose load test. The fasting tear glucose level did not appear to be a satisfactory index for classifying a person as a diabetic or normal. It was found that after the peroral glucose load test a tear glucose level at 11 mg/100 ml (0.61 mmol/l) resulted in only 4.6% of the diabetics being missed, while 5.8% of the normal persons were misclassified as diabetics.
Pharmacokinetic Interactions of Concomitant Administration of Febuxostat and NSAIDs (2006)

To evaluate the effect of febuxostat on the pharmacokinetics of indomethacin and naproxen and vice versa, 2 multiple-dose, 3-period crossover studies were performed in healthy subjects. In study 1, subjects received febuxostat 80 mg once daily, indomethacin 50 mg twice daily, or both. In study 2, subjects received febuxostat 80 mg, naproxen 500 mg twice daily, or both. Twenty-four-hour blood samples were collected on day 5 in study 1 and day 7 in study 2. In study 1, 90% confidence intervals of geometric mean ratios for maximum plasma concentration (Cmax) and area under the curve (AUC) were within the 0.80 to 1.25 no-effect range for febuxostat and indomethacin. In study 2, 90% confidence intervals for febuxostat C(max) and AUC extended above that range, with increases of 28% and 40% in Cmax and AUC24, respectively. However, 90% confidence intervals for naproxen C(max) and AUC were within the 0.80 to 1.25 range. Febuxostat had no effect on the plasma pharmacokinetics of indomethacin and naproxen. Similarly, indomethacin had no effect on the plasma pharmacokinetics of febuxostat. Although naproxen caused an increase in plasma exposure to febuxostat, this increase is not expected to be clinically significant. Therefore, based on the plasma pharmacokinetic data in healthy subjects, febuxostat may be administered with indomethacin or naproxen with no dose adjustments for febuxostat, indomethacin, or naproxen.
Vectors for rapid selection of integrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1 (1999)

Plasmids with different selectable markers were constructed and used to transform the Hansenula polymorpha strain DL1. It was shown that, depending on the host mutant strain, the use of these plasmids enables rapid selection of transformants with plasmids integrated in low (1–2), moderate (6–9) or high (up to 100) copy numbers. The vectors and mutants described are potentially useful for the construction of efficient producers of heterologous proteins in H. polymorpha. Copyright © 1999 John Wiley & Sons, Ltd.