gebhart/patrepeval huggingface dataset:
Design, Execution, and Evaluation of a "Typical" Marcellus Shale Slickwater Stimulation: A Case History (2008)

Abstract In 2004 the first Appalachian Basin Marcellus Shale gas well stimulation utilizing a large slickwater treatment was performed in southwestern Pennsylvania. Since that time, based upon various publically reported results, it has become the most widely discussed play in the basin with numerous operators drilling and completing Marcellus wells in Pennsylvania, West Virginia, Ohio, and New York. This wide geographic range has led to numerous different completion schemes being utilized as with geographic variation comes geologic variability within the formation itself. A primary topic of discussion has been determining the optimal size and type of stimulation treatment for a given area. This paper will discuss the stimulation treatment designed and performed on a Marcellus well operated by Guardian Exploration LLC.
Total Synthesis of an Immunosuppressive Glycolipid, (2S,3S,4R)-1-O- (α-d-Galactosyl)-2- tetracosanoylamino-1,3,4-nonanetriol (2005)

[reaction: see text] A practical and efficient total synthesis of (2S,3S,4R)-1-O-(alpha-d-galactosyl)-2-tetracosanoylamino-1,3,4-nonanetriol, OCH 1b, a potential therapeutic candidate for Th1-mediated autoimmune diseases, is described. The synthesis incorporates direct alkylation onto epoxide 5 and stereospecific halide ion catalyzed alpha-glycosidation reaction. A key intermediate 10 was obtained in only eight steps and 37% overall yield from commercially available d-arabitol 2, and the total synthesis of 1b was accomplished in 12 steps and 19% overall yield. This method will enable the synthesis of a variety of phytosphingolipids, especially that with the shorter sphingosine side chain than 1a, in a highly stereoselective manner.
System design for optical PPM communications with diode combining (1988)

The most efficient way to encode laser diodes for maximum data rate is studied. Three different system architectures are considered, with combining achieved by dichroic mirrors operating in conjunction with a pulse-position-modulated (PPM) format. The basic criterion is to maximize the data rate with increasing number of diodes, while maintaining diode power constraints and decoding bit error probability. The three systems are: power combining into a single pulse, followed by PPM encoding; parallel channels, in which each diode is separately PPM encoded; and color coding, in which diodes are encoded over a common wavelength-time slot alphabet. Data rate equations are presented as a function of the number of diodes, mirror combining losses, PPM alphabet size, and the operating optical signal-to-noise ratio.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Chemerin Contributes to Inflammation by Promoting Macrophage Adhesion to VCAM-1 and Fibronectin through Clustering of VLA-4 and VLA-5 (2010)

Chemerin is a potent macrophage chemoattractant protein. We used murine peritoneal exudate cells (PECs) in adhesion, flow cytometry, and confocal microscopy assays to test the hypothesis that chemerin can also contribute to inflammation by promoting macrophage adhesion. Chemerin stimulated the adhesion of PECs to the extracellular matrix protein fibronectin and to the adhesion molecule VCAM-1 within a minute, with an EC(50) of 322 and 196 pM, respectively. Experiments using pertussis toxin and PECs from ChemR23(-/-) mice demonstrated that chemerin stimulated the adhesion of macrophages via the Gi protein-coupled receptor ChemR23. Blocking Abs against integrin subunits revealed that 89% of chemerin-stimulated adhesion to fibronectin was dependent on increased avidity of the integrin VLA-5 (alpha(5)beta(1)) and that 88% of adhesion to VCAM-1 was dependent on increased avidity of VLA-4 (alpha(4)beta(1)). Although chemerin was unable to induce an increase in integrin affinity as judged by the binding of soluble ligand, experiments using confocal microscopy revealed an increase in valency resulting from integrin clustering as the mechanism responsible for chemerin-stimulated macrophage adhesion. PI3K, Akt, and p38 were identified as key signaling mediators in chemerin-stimulated adhesion. The finding that chemerin can rapidly stimulate macrophage adhesion to extracellular matrix proteins and adhesion molecules, taken together with its ability to promote chemotaxis, suggests a novel role for chemerin in the recruitment and retention of macrophages at sites of inflammation.
Traffic Detector Handbook: Third Edition - Volume II (2006)

The objective of this Handbook is to provide a comprehensive resource for selecting, designing, installing, and maintaining traffic sensors for signalized intersections and freeways. It is intended for use by traffic engineers and technicians having responsibility for traffic sensors, whether in-roadway or over-roadway sensors. These two families of sensors have different characteristics and thus corresponding advantages and disadvantages that are discussed throughout the Handbook. Topics covered include sensor technology, applications, in-roadway sensor design, installation techniques, and maintenance. The sensor technology chapter discusses the operation and uses of inductive loop detectors, magnetic sensors and detectors, video image processors, microwave radar sensors, laser radars, passive infrared and passive acoustic array sensors, and ultrasonic sensors, plus combinations of sensor technologies. The sensor application topics address safety, operational performance, multimodal issues, and physical and economic factors that the practitioner should consider. Appendixes include research, background papers, and implementation guidance. The information contained in this Handbook is based on the latest research available on treatments and best practices in use by the surveyed jurisdictions. References are provided for the student, practitioner, researcher, or decision maker who wishes to learn more about a particular subject.
Pharmacological interventions for agitation in patients with traumatic brain injury: protocol for a systematic review and meta-analysis (2016)

PROSPERO CRD42016033140.
Binding and Stability Determinants of the PPARγ Nuclear Receptor−Coactivator Interface As Revealed by Shotgun Alanine Scanning and in Vivo Selection (2006)

We modified an existing selection for protein-protein interactions based on the fragment complementation of the enzyme DHFR. Using shotgun alanine scanning in conjunction with this selection, we analyzed the interaction of the nuclear receptor PPARgamma with two peptides derived from nuclear receptor coactivators SRC1 and TRAP220. A large binding epitope stretching between and including the charge clamp residues K301 and E471 of PPARgamma was identified as necessary for PPARgamma-coactivator interaction. To decouple protein stability from the propensity to form a receptor-coactivator interface, libraries of PPARgamma variants generated by shotgun scanning were further processed using a high-throughput screen measuring their in vivo stabilities. Our findings demonstrate that many of the residues that make up the binding epitope of PPARgamma are also crucial for the stability of the PPARgamma.
Α genetic fusion construct between the tetanus toxin C fragment and the lysosomal acid hydrolase β‐glucuronidase expresses a bifunctional protein with enhanced secretion and neuronal uptake (2005)

The neurotropic atoxic fragment of tetanus toxin has been used as a carrier for transporting macromolecules into neurons but all studies to date have tested cytosolic proteins. In this study we investigated the effect of a genetic addition of the tetanus toxin C fragment sequence to a lysosomal enzyme which contains a signal sequence for insertion into the membrane-bound compartment and must be extensively modified in the endoplasmic reticulum (ER) and Golgi to attain functionality. In-frame fusion constructs between the atoxic C fragment and beta-glucuronidase were compared with the wild-type enzyme for: (i) enzymatic activity; (ii) heat stability; (iii) pH dependence; (iv) specific activity; (v) apparent molecular mass and (vi) receptor-mediated uptake by fibroblasts and neurons. The modified proteins had biochemical properties similar to wild-type enzyme but exhibited different enzyme secretion profiles. Addition of the secreted fusion enzyme to cultures of primary neurons showed significantly increased neuronal uptake of the modified protein compared with the wild-type, demonstrating the bifunctionality of the chimeric molecule.
Landscape of EGFR-Dependent and -Independent Resistance Mechanisms to Osimertinib and Continuation Therapy Beyond Progression in EGFR -Mutant NSCLC (2018)

Osimertinib resistance is associated with diverse, predominantly EGFR-independent genomic alterations. Continuation of osimertinib after progression, alone or in conjunction with radiotherapy, may provide prolonged clinical benefit in selected patients.<i>See related commentary by Devarakonda and Govindan, p. 6112</i>.
Maternal serum hCG and fetal nuchal translucency thickness for the prediction of fetal trisomies in the first trimester of pregnancy (1995)

An improved estimate of risk for fetal trisomies at 10 to 13 weeks gestation can be derived by combining data on maternal age, maternal serum total or free beta-hCG and fetal nuchal translucency thickness.
Coronavirus Neurovirulence Correlates with the Ability of the Virus To Induce Proinflammatory Cytokine Signals from Astrocytes and Microglia (2004)

The molecular and cellular basis of coronavirus neurovirulence is poorly understood. Since neurovirulence may be determined at the early stages of infection of the central nervous system (CNS), we hypothesize that it may depend on the ability of the virus to induce proinflammatory signals from brain cells for the recruitment of blood-derived inflammatory cells. To test this hypothesis, we studied the interaction between coronaviruses (mouse hepatitis virus) of different neurovirulences with primary cell cultures of brain immune cells (astrocytes and microglia) and mouse tissues. We found that the level of neurovirulence of the virus correlates with its differential ability to induce proinflammatory cytokines (interleukin 12 [IL-12] p40, tumor necrosis factor alpha, IL-6, IL-15, and IL-1beta) in astrocytes and microglia and in mouse brains and spinal cords. These findings suggest that coronavirus neurovirulence may depend on a novel discriminatory ability of astrocytes and microglia to induce a proinflammatory response in the CNS.
Conversion of vegetable oils and animal fats into paraffinic cetane enhancers for diesel fuels (1995)

The two principal methods of producing biodiesel fuels are (a) transesterification of vegetable oils and animal fats with a monohydric alcohol, and (b) direct hydrotreating of tree oils, vegetable oils and animal fats. The patented hydrotreating technology is based on the catalytic processing of biomass oils and fats with hydrogen, under elevated temperature and pressure conditions. The typical mix of hydrotreated products is as follows: 5-15% light distillate (naphta), 40-60% middle distillate (cetane), 5-15% heavy distillate and 5-10% burner gas. The naptha fraction may be used as a gasoline supplement. The middle distillate is designed for use as a cetane booster for diesel fuels. Both heavy distillate and light hydrocarbon gases are usable as power boiler fuels. Typically, the cetane enhancer would be admixed with diesel fuel in the range of 5 to 30% by volume. This new diesel blend meets the essential quality characteristics of the basic diesel fuel, for direct use in diesel engines without any modifications. The basic hydrotreatment technology has been evaluated further in the laboratory on degummed soya oil, yellow grease and animal tallow. The preliminary findings suggest that the technology can provide efficient conversion of these materials into cetane enhancers for diesel fuels.
Acetate utilization as a survival strategy of peat‐inhabiting Methylocystis spp. (2010)

Representatives of the genus Methylocystis are traditionally considered to be obligately methanotrophic bacteria, which are incapable of growth on multicarbon substrates. Here, we describe a novel member of this genus, strain H2s, which represents a numerically abundant and ecologically important methanotroph population in northern Sphagnum-dominated wetlands. This isolate demonstrates a clear preference for growth on methane but is able to grow slowly on acetate in the absence of methane. Strain H2s possesses both forms of methane monooxygenase (particulate and soluble MMO) and a well-developed system of intracytoplasmic membranes (ICM). In cells grown for several transfers on acetate, these ICM are maintained, although in a reduced form, and mRNA transcripts of particulate MMO are detectable. These cells resume their growth on methane faster than those kept for the same period of time without any substrate. Growth on acetate leads to a major shift in the phospholipid fatty acid composition. The re-examination of all type strains of the validly described Methylocystis species showed that Methylocystis heyeri H2(T) and Methylocystis echinoides IMET10491(T) are also capable of slow growth on acetate. This capability might represent an important part of the survival strategy of Methylocystis spp. in environments where methane availability is variable or limited.
Effects of arginine vasotocin, oxytocin, and arginine vasopressin on steroid-induced surges of luteinizing hormone and prolactin in ovariectomized rats (1980)

Abstract. Several studies have indicated that arginine vasotocin (AVT), a nonapeptide closely related to vasopressin (ADH) and oxytocin (OT), may act as a pineal antigonadotrophic factor. The present studies were designed to investigate the effects of AVT on the luteinizing hormone (LH) and prolactin (Prl) release induced by sequential steroid priming of ovariectomized (OVX). rats. Sprague-Dawley rats were used 6–8 weeks post-OVX. After implantation of an intra-atrial bleeding catheter, animals were primed with 5 μg of oestradiol benzoate (EB) at 08.00 h on the next morning. Forty-eight hours later 1.5 mg of progesterone (P) was injected and animals were divided into groups which received either saline, AVT (1 of 4 doses), ADH, or OT. The saline or peptides were infused via the intra-atrial catheter at 10.00, 11.00, 12.00, and 13.00 h. Hourly blood sampling was performed at 11.00–18.00 h, and at 21.00 h. The 11.00, 12.00, and 13.00 h samples were taken 10 min after saline or peptide infusion. LH and Prl responses to the peptide infusions could be divided into pre-surge and surge effects. AVT caused a slight, but significant elevation of the normally low levels of LH and Prl which occurred before the onset of their surges. Only the highest dose of AVT (1.0 μg) blocked the LH surge. ADH, however, was capable of stimulating LH and Prl release during the pre-surge period and of inhibiting the LH surge. AVT at a dose of 0.5 or 1.0 μg specifically blocked the onset of the Prl surge, causing Prl to drop to its lowest level at 14.00 h - the time at which Prl levels were maximal in saline-treated animals. After this initial inhibition, however, Prl levels rebounded to show a delayed surge. OT infusion, on the other hand, caused a significant augmentation of the Prl surge. These data indicate that AVT may specifically block the onset of the Prl surge seen after sequential steroid priming of OVX rats, while OT may facilitate the Prl surge.
Optimization and delivery of plasmid DNA for vaccination (2006)

Vaccination with DNA is one of the most promising novel immunization techniques against a variety of pathogens and tumors, for which conventional vaccination regimens have failed. DNA vaccines are able to stimulate both arms of the immune system simultaneously, without carrying the safety risks associated with live vaccines, therefore representing not only an alternative to conventional vaccines but also significant progress in the prevention and treatment of fatal diseases and infections. However, translation of the excellent results achieved in small animals to similar success in primates or large animals has so far proved to be a major hurdle. Moreover, biosafety issues, such as the removal of antibiotic resistance genes present in plasmid DNA used for vaccination, remain to be addressed adequately. This review describes strategies to improve the design and production of conventional plasmid DNA, including an overview of safety and regulatory issues. It further focuses on novel systems for the optimization of plasmid DNA and the development of diverse plasmid DNA delivery systems for vaccination purposes.
Four enzymes define the incorporation of coenzyme A in thienamycin biosynthesis (2008)

The enzymatic activities of three proteins encoded by the thienamycin gene cluster of Streptomyces cattleya (ThnR, ThnH, and ThnT) have been shown to incrementally cleave CoA to afford the active side-chain component of the beta-lactam antibiotic thienamycin. These results supersede proposals based on earlier radiochemical incorporation experiments. For 20 years it has been thought that cysteine was directly incorporated into the antibiotic. Specific, stepwise truncation of CoA to 4-phosphopantetheine, pantetheine, and finally cysteamine was observed with ThnR, ThnH, and ThnT, respectively, in a series of coupled enzymatic assays. Pantetheinylated carbapenams were synthesized to address possible thienamycin biosynthetic intermediates and were shown to be effective substrates for the pantetheine-cleaving enzyme ThnT. Finally, a fourth gene, thnF, was shown to encode a protein capable of N-acetylating a model compound containing cysteamine in the presence of acetyl-CoA, consistent with the production of the S. cattleya cometabolite, N-acetylthienamycin. Taken together, these four enzymes are proposed to siphon CoA from primary metabolism to create the side chains for the predominant S. cattleya carbapenems, thienamycin and N-acetylthienamycin, in a process likely to be general for the broader class of these antibiotics.
Polysaccharides of Chlorella pyrenoidosa (1962)

HEAVY-METAL IONS AND p-GLUCURONIDASE 509
Electromagnetic Heating Methods for Heavy Oil Reservoirs (2000)

Abstract The most widely used method of thermal oil recovery is by injecting steam into the reservoir. A well-designed steam injection project is very efficient in recovering oil, however its applicability is limited in many situations. Simulation studies and field experience has shown that low injectivity reservoirs and small thickness of the oil-bearing zone limit the performance of steam injection. This paper discusses alternative methods of transferring heat to heavy oil reservoirs, based on electromagnetic energy. We present a detailed analysis of low frequency electric resistive (ohmic) heating and higher frequency electromagnetic heating (microwave frequency). We show the applicability of electromagnetic heating in two example reservoirs. The first reservoir has thin sand zones separated by impermeable shale layers and viscous oil (9541 cp at initial reservoir conditions). We model preheating the reservoir with low frequency current using two horizontal electrodes, before injecting steam. The second reservoir has low permeability and oil viscosity of 33 cp at initial reservoir conditions. In this case we use a high frequency microwave antenna located 30 ft from the producing well as the heat source. Simulation results presented in this paper suggest that electromagnetic heating may be a good stimulation technique for heavy oil reservoirs with low injectivity or with thin payzones. We identify the parameters which are critical for electromagnetic heating. We also discuss past field applications of electromagnetic heating as well as technical challenges and limitations.
Gap Junction Modulation in Rat Uterus. III. Structure-Activity Relationships of Estrogen Receptor-Binding Ligands on Myometrial and Serosal Cells1 (1987)

A number of steroidal and nonsteroidal estrogen receptor-binding ligands were tested for their ability to affect the formation and internalization of gap junctions in hypophysectomized rat uterine myometrial and serosal cells. Potent estrogen, including diethylstilbestrol, estradiol benzoate (EB), estradiol-17 beta, and the weak estrogens, estriol and estrone, stimulate formation of macular and annular gap junctions in myometrium in a dose-dependent fashion when administered in daily injections over 5 days. Induction of annular gap junctions in the uterine serosal epithelium follows a similar dose-dependent pattern of estrogen stimulation but requires lower levels of hormone to initiate the response. In myometrium, differential stimulation of circular and longitudinal myometrial cell layers was observed, with 3 to 5 times more gap junctions detected in the circular than in the longitudinal layer. Progesterone, estriol, or estrone suppress the myometrial gap junction response to EB when administered concurrently with EB. However, the EB-stimulated appearance of myometrial cell gap junctions was blocked when the progesterone-to-estrogen ratio exceeded 100:1. The estrogen receptor-binding androgens, 5 alpha-androstane-3 beta,17 beta-diol (Adiol) and delta 5-androstene-3 beta,17 beta-diol failed to induce myometrial gap junctions at doses up to 5 mg/day for 5 days, whereas Adiol did induce annular gap junctions in the serosal cells at the highest dosage tested. Of the triphenylethylene derivatives and related compounds evaluated, including mixed isomers of tamoxifen and CI 628, the cis (zuclomiphene, ZUC) and trans (enclomiphene) isomers of clomiphene citrate, and a fixed-ring antiestrogen, nafoxidine, only ZUC was able to induce gap junctions in myometrial and serosal cells. These studies indicate that induction of gap junctions in rat uterine myometrial cells is an estrogen-dependent response that requires higher levels of estrogen than other estrogen-dependent target cell responses in the rodent uterus.
Recent developments in indole ring synthesis—methodology and applications (1994)

G. W. Gribble, Contemp. Org. Synth., 1994, 1, 145 DOI: 10.1039/CO9940100145
Ultrathin-layer gel electrophoresis of biopolymers (2000)

Emerging need for large-scale, high-resolution analysis of biopolymers, such as DNA sequencing polymerase chain reaction, (PCR) product sizing, single nucleotide polymorphism (SNP) hunting and analysis of protein molecules necessitated the development of automated and high-throughput gel electrophoresis based methods enabling rapid, high-performance separations in a wide molecular weight range. Scaling down electric field mediated separation processes supports higher throughput due to the applicability of higher voltages, thus speeding up analysis time. Indeed, efforts in miniaturization resulted in faster, easier, less costly and more convenient analyses, fulfilling the needs of the emerging biotechnology industry for microscale and massively parallel assays. The two primary approaches in miniaturizing electrophoresis dimensions are the capillary and microslab formats. This latter one evolved towards ultrathin-layer gel electrophoresis which is, except from the thickness of the separation platform, slightly in the upper side of the scale, resulting in considerably easier handling. Ultrathin-layer gel electrophoresis combines the advantages of conventional slab-gel electrophoresis (multilane format) and capillary gel electrophoresis (rapid, high-efficiency separations). It is readily automated, automatic versions of it have been extensively used for large-scale DNA sequencing in the Human Genome Project and more recently became popular in high throughput DNA fragment analysis. Ultrathin-layer techniques are the first step towards the wider use of electrophoresis microchips in perfecting a user-friendly interface between the user and the microdevice.
Rehabilitation in progressive supranuclear palsy: Effectiveness of two multidisciplinary treatments (2017)

This trial was registered on ClinicalTrials.gov, NCT02109393.