gebhart/patrepeval huggingface dataset:
P-lacW insertional mutagenesis on the second chromosome of Drosophila melanogaster: isolation of lethals with different overgrowth phenotypes. (1993)

A single P-element insertional mutagenesis experiment was carried out for the second chromosome of Drosophila melanogaster using the P-lacW transposon. Out of 15,475 insertions on the second chromosome, 2,308 lethal and 403 semilethal mutants (altogether 2,711) were recovered. After eliminating clusters, 72% of the mutants represent independent insertions. Some of the mutants with larval, prepupal or pupal lethal phases have a prolonged larval period and show gradual overgrowth of the imaginal discs, brain and/or the hematopoietic organs (lymph glands). In this paper, 16 overgrowth mutants are described. As revealed by in situ hybridization, none of the mutations corresponds to any of the previously known overgrowth mutations on the second chromosome.
Fetal magnetocardiography measurements with an array of microfabricated optically pumped magnetometers (2015)

Following the rapid progress in the development of optically pumped magnetometer (OPM) technology for the measurement of magnetic fields in the femtotesla range, a successful assembly of individual sensors into an array of nearly identical sensors is within reach. Here, 25 microfabricated OPMs with footprints of 1 cm(3) were assembled into a conformal array. The individual sensors were inserted into three flexible belt-shaped holders and connected to their respective light sources and electronics, which reside outside a magnetically shielded room, through long optical and electrical cables. With this setup the fetal magnetocardiogram of a pregnant woman was measured by placing two sensor belts over her abdomen and one belt over her chest. The fetal magnetocardiogram recorded over the abdomen is usually dominated by contributions from the maternal magnetocardiogram, since the maternal heart generates a much stronger signal than the fetal heart. Therefore, signal processing methods have to be applied to obtain the pure fetal magnetocardiogram: orthogonal projection and independent component analysis. The resulting spatial distributions of fetal cardiac activity are in good agreement with each other. In a further exemplary step, the fetal heart rate was extracted from the fetal magnetocardiogram. Its variability suggests fetal activity. We conclude that microfabricated optically pumped magnetometers operating at room temperature are capable of complementing or in the future even replacing superconducting sensors for fetal magnetocardiography measurements.
Highly Decorated Lignins in Leaf Tissues of the Canary Island Date Palm Phoenix canariensis (2017)

The cell walls of leaf base tissues of the Canary Island date palm (<i>Phoenix canariensis</i>) contain lignins with the most complex compositions described to date. The lignin composition varies by tissue region and is derived from traditional monolignols (ML) along with an unprecedented range of ML conjugates: ML-acetate, ML-benzoate, ML-<i>p</i>-hydroxybenzoate, ML-vanillate, ML-<i>p</i>-coumarate, and ML-ferulate. The specific functions of such complex lignin compositions are unknown. However, the distribution of the ML conjugates varies depending on the tissue region, indicating that they may play specific roles in the cell walls of these tissues and/or in the plant's defense system.
An Improved Single-Pellet Reactor to Study the Interaction of Kinetics with Mass Transfer Effects in Heterogeneous Catalysis (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn Improved Single-Pellet Reactor to Study the Interaction of Kinetics with Mass Transfer Effects in Heterogeneous CatalysisL. Louis Hegedus and Eugene E. PetersenCite this: Ind. Eng. Chem. Fundamen. 1972, 11, 4, 579–584Publication Date (Print):November 1, 1972Publication History Published online1 May 2002Published inissue 1 November 1972https://pubs.acs.org/doi/10.1021/i160044a025https://doi.org/10.1021/i160044a025research-articleACS PublicationsRequest reuse permissionsArticle Views173Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
2-[(2-Pyridylmethyl)sulfinyl]-1H-thieno[3,4-d]imidazoles. A novel class of gastric H+/K+-ATPase inhibitors (1992)

2-[(2-Pyridylmethyl)sulfinyl]thienoimidazoles were synthesized and investigated as potential inhibitors of gastric H+/K(+)-ATPase. The [3,4-d] isomers of the two possible thienoimidazole series were found to be potent inhibitors of gastric acid secretion in vitro and in vivo. Structure-activity relationships indicate that especially lipophilic alkoxy, benzyloxy, and phenoxy substituents with additional electron-demanding properties in the 4-position of the pyridine moiety combined with an unsubstituted thieno[3,4-d]imidazole lead to highly active compounds with a favorable chemical stability. Various substitution patterns in the thieno[3,4-d]imidazole moiety result in lower biological activity. The heptafluorobutyloxy derivative saviprazole (HOE 731, 5d) was selected for further development and is currently undergoing clinical evaluation. Comprehensive pharmacological studies indicate a pharmacodynamic profile different to omeprazole, the first H+/K(+)-ATPase blocker introduced on the market.
Estudio histoquimico de los cambios en la expresion de glicanos durante una gingivitis experimental en humanos (1996)

En tres voluntarios sanos se indujo un modelo de gingivitis en la hemiarcada superior derecha (14 dias sin ninguna medida de higiene oral); la hemiarcada superior izquierda sirvio como control.
The validity of psychometric instruments for detection of dementia in the elderly general population (1998)

There is no perfect instrument for screening and diagnosis of dementia. Different threshold scores for different purposes were provided in the present study. Recommendations for improving the validity of the Delayed Word List Learning Task for discriminating dementia and cognitive health include the expansion of list length and shortening of delay.
In vivo administration of anti-CD3 monoclonal antibody can activate immune responses thus preventing malignant tumor growth. (1988)

Anti-CD3 monoclonal antibodies (mAb) have been shown to suppress T cell-mediated immune responses both in vitro and in vivo. However, in vitro studies with these antibodies have also demonstrated that they possess potent mitogenic properties, raising the possibility that they might be capable of potentiating immune responses in vivo. In this regard, we have recently shown that an anti-CD3 mAb can activate murine T cells in vivo. Furthermore, low doses of antibody induce interleukin 2 (IL-2) receptor expression and enhanced proliferation to allogeneic major histocompatibility complex (MHC) antigen without detectable modulation or blocking of the T cell receptor and without suppression of T cell-mediated immune responses. In light of these findings, we investigated the ability of low dose anti-CD3 to enhance an anti-tumor response directed against the malignant murine UV-induced skin tumor, 1591-Pro-4L. Low dose anti-CD3 administration resulted in enhanced in vitro anti-tumor activity and prevented tumor outgrowth in approximately two-thirds of animals treated at the time of tumor inoculation. Furthermore, these animals displayed lasting tumor-specific immunity. These results suggest that anti-CD3 mAb can be utilized for the enhancement of anti-tumor responses in vivo and may have general application in the treatment of immunodeficiency.
A Comparison of Murine and Human Factor XI (1997)

Factor XI is a plasma glycoprotein that is required for contact activation initiated fibrin formation in vitro and for normal hemostasis in vivo. In preparation for developing a mouse model of factor XI deficiency to facilitate investigations into this protease's contributions to coagulation, we cloned the complementary DNA for murine factor XI, expressed the protein in a mammalian expression system, and compared its properties with human recombinant factor XI. The 2.8-kb murine cDNA codes for a protein of 624 amino acids with 78% homology to human factor XI. Both recombinant murine and human factor XI are 160 kD homodimers comprised of two 80 kD polypeptides connected by disulfide bonds. Murine factor XI shortens the clotting time of human factor XI deficient plasma in an activated partial thromboplastin time assay, with a specific activity 50% to 70% that of the human protein. In a purified system, murine factor XI is activated by human factor XIIa and thrombin in the presence of dextran sulfate. Murine factor XI differs from human factor XI in that it undergoes autoactivation slowly in the presence of dextran sulfate. This is due primarily to murine factor XIa preferentially cleaving a site on zymogen factor XI within the light chain, rather than the activation site between Arg371 and Val372. Northern blots of polyadenylated messenger RNA show that murine factor XI message is expressed, as expected, primarily in the liver. In contrast, messenger RNA for human factor XI was identified in liver, pancreas, and kidney. The studies show that murine and human factor XI have similar structural and enzymatic properties. However, there may be variations in tissue specific expression and subtle differences in enzyme activity across species.
Cast Natural Rubber Films Comprising Triethyl Citrate (2013)

The various triethyl citrate-loaded natural rubber (NR) films were prepared by casting technique employing dichloromethane (DCM) as solvent. The wettability, mechanical properties and topology of prepared dry films were determined. The contact angle of distilled water onto the cast films was measured using the goniometer. Triethyl citrate-loaded NR films exhibited the continuous porous structure. The presence of triethyl citrate in NR films promoted wettability and surface free energy whereas the tensile strength decreased. The unique porous topography of triethyl citrate-loaded NR film exhibited the potential for applying this material as the scaffold or controlled drug delivery devices in the future.
4-[18F]Fluoro-m-hydroxyphenethylguanidine: A Radiopharmaceutical for Quantifying Regional Cardiac Sympathetic Nerve Density with Positron Emission Tomography (2013)

4-[(18)F]Fluoro-m-hydroxyphenethylguanidine ([(18)F]4F-MHPG, [(18)F]1) is a new cardiac sympathetic nerve radiotracer with kinetic properties favorable for quantifying regional nerve density with PET and tracer kinetic analysis. An automated synthesis of [(18)F]1 was developed in which the intermediate 4-[(18)F]fluoro-m-tyramine ([(18)F]16) was prepared using a diaryliodonium salt precursor for nucleophilic aromatic [(18)F]fluorination. In PET imaging studies in rhesus macaque monkeys, [(18)F]1 demonstrated high quality cardiac images with low uptake in lungs and the liver. Compartmental modeling of [(18)F]1 kinetics provided net uptake rate constants Ki (mL/min/g wet), and Patlak graphical analysis of [(18)F]1 kinetics provided Patlak slopes Kp (mL/min/g). In pharmacological blocking studies with the norepinephrine transporter inhibitor desipramine (DMI), each of these quantitative measures declined in a dose-dependent manner with increasing DMI doses. These initial results strongly suggest that [(18)F]1 can provide quantitative measures of regional cardiac sympathetic nerve density in human hearts using PET.
A keyword spotter which incorporates neural networks for secondary processing (2002)

Experiments using restricted Coulomb energy (RCE) and backward error propagation trained artificial neural networks (ANNs) for secondary processing in a keyword spotting application are described. Several types and configurations of neural networks are explored, including single, multiple, and hybrid networks. Several feature space transformations are used to permit the ANNs to examine the potential word in several time-invariant formats. The best performance is obtained using a multiple RCE network structure, which improves performance an average of 5% over a range of false alarm rates. The effectiveness of several ANNs as feature extraction mechanisms and as pattern classifiers is discussed relative to the keyword spotting problem. Issues pertaining to the complexity and required training time of the ANN structures are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Comparative Analysis of Folate Derived PET Imaging Agents with [18F]-2-Fluoro-2-deoxy-d-glucose Using a Rodent Inflammatory Paw Model (2013)

Activated macrophages play a significant role in initiation and progression of inflammatory diseases and may serve as the basis for the development of targeted diagnostic methods for imaging sites of inflammation. Folate receptor beta (FR-β) is differentially expressed on activated macrophages associated with inflammatory disease states yet is absent in either quiescent or resting macrophages. Because folate binds with high affinity to FR-β, development of folate directed imaging agents has proceeded rapidly in the past decade. However, reports of PET based imaging agents for use in inflammatory conditions remain limited. To investigate whether FR-β expressing macrophages could be exploited for PET based inflammatory imaging, two separate folate-targeted PET imaging agents were developed, 4-[18F]-fluorophenylfolate and [68Ga]-DOTA-folate, and their ability to target activated macrophages were examined in a rodent inflammatory paw model. We further compared inflamed tissue uptake with 2-[18F]fluoro-2-deoxy-d-glucose ([18F]-FDG). microPET analysis demonstrated that both folate-targeted PET tracers had higher uptake in the inflamed paw compared to the control paw. When these radiotracers were compared to [18F]-FDG, both folate PET tracers had a higher signal-to-noise ratio (SNR) than [18F]-FDG, suggesting that folate tracers may be superior to [18F]-FDG in detecting diseases with an inflammatory component. Moreover, both folate-PET imaging agents also bind to FR-α which is overexpressed on multiple human cancers. Therefore, these folate derived PET tracers may also find use for localizing and staging FR+ cancers, monitoring response to therapy, and for selecting patients for tandem folate-targeted therapies.
Angiogenesis from the Eighth Cranial Nerve to Vestibular Schwannomas (1996)

Vascularization between vestibular schwannomas and the adjacent eighth cranial nerve was examined in order to elucidate the angiogenic effect of these tumours. Specimens from 12 patients were examined by light microscopy and immunohistochemistry (series 1), while specimens from another 17 patients were examined by light and transmission electron microscopy (series 2). Slight to marked angiogenesis was found in 5 patients from series 1 and 7 patients from series 2. Ultrastructural examination indicated that these blood vessels were more fragile than those in the normal eighth cranial nerve. These findings suggested that sudden hearing loss in vestibular schwannoma patients may be related to the disruption of such blood vessels. In addition, surgical injury to these blood vessels may cause postoperative hearing loss despite preservation of the cochlear nerve.
Scheduling in switches with small internal buffers (2005)

Unbuffered crossbars or switching fabrics contain no internal buffers, and function using only input (VOQ) and possibly output queues. Schedulers for such switches are complex, and introduce increased delay at medium loads, because they have to admit at most one cell per input and per output, during each time slot. Buffered crossbars, on the other hand, contain Q sufficient internal buffering (N <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> buffers) to allow independent schedulers to concurrently forward packets to the same output from any number of inputs. These architectures represent the two extremes in a range of solutions, which we examine here; although intermediate points in this range are of reduced practical interest for crossbars, they are nevertheless quite interesting for switching fabrics, and they may be of interest for optical switches. We find that tolerating two cells per-output per time-slot, using small buffers inside the switch or fabric, suffices for independent and efficient scheduling. First, we introduce a novel "request-grant" credit protocol, enabling N inputs to share a small switch buffer. Then, we apply this protocol to a switch with N such buffers, one per output, and we consider the resulting scheduling problem. Interestingly, this looks like unbuffered crossbar schedulers, but it is much simpler because it comprises independent schedulers that can be pipelined. We show that individual buffer sizes do not need to grow, neither with switch size nor with propagation delay. Through simulations, we study performance as a function of the number of cells allowed per-output per-time-slot. For one cell, the switch performs very close to the iSLIP unbuffered crossbar with one iteration. For more cells, performance improves quickly; for 12 cells, packet delay under (smooth) uniform load is practically as low as ideal output queueing. Under unbalanced load, throughput is superior to buffered crossbars, due to better buffer sharing
Micropatterning Hydrophobic Liquid on a Porous Polymer Surface for Long‐Term Selective Cell‐Repellency (2013)

A simple method to form precise micropatterns of hydrophobic liquids using porous hydrophilic-hydrophobic substrates is presented. The micropatterns of hydrophobic liquid exhibit long-term stability, excellent cell-repellency, no cytotoxicity, and are more efficient than conventional PEG or superhydrophobic surfaces in controlling eukaryotic cell adhesion.
High Strength/High Modulus Aromatic Heterocyclic ABA Block Copolymers (1987)

This paper describes our research on the synthesis and properties of ABA block copolymers composed of a rigid-rod (B) block for reinforcement and a flexible coil (A) block as the matrix. In addition to better processability (Ccr), it was anticipated that the block copolymer would have better mechanical strength than those from physical blends of the rigid-rod/flexible coil composition. In the block copolymer, the rigid-rod constituent would be chemically bonded to the flexible coil matrix, while in the physically blended molecular composite the reinforcing rigid-rod polymer is only physically entangled in the matrix. Hence, the translation of stress or strain from the reinforcing rigid-rod molecule in the matrix should be more efficient in the block copolymer than in the physical blend. This would not only provide higher tensile strength, but also fracture toughness and dimensional stability.
Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein–Barr virus (2001)

The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin. Mcm2, a subunit of the MCM replication licensing complex, was found to associate with oriP during G(1) and to dissociate from it during S phase. The detection of ORC and Mcm2 at oriP was shown to require the presence of the 120-bp replicator of oriP. Licensing and initiation of replication at oriP of EBV thus seem to be mediated by ORC. This is an example of a virus apparently using ORC and associated factors for the propagation of its genome.
Intramyocardial Gene Therapy with Naked DNA Encoding Vascular Endothelial Growth Factor Improves Collateral Flow to Ischemic Myocardium (1999)

Both VEGF protein and VEGF DNA in combination with an adenoviral vector have been shown to enhance collateral formation in a porcine model of chronic myocardial ischemia. We sought to determine whether direct intramyocardial injection of naked DNA encoding for VEGF could similarly improve myocardial perfusion. Initially, 23 nonischemic pigs received either 200 microg of plasmid DNA encoding beta-galactosidase (pCMVbeta, n = 11) or 500 microg of phVEGF165 (n = 12) into four separate sites in the myocardium via a small anterolateral thoracotomy incision in the fourth intercostal space. Two additional groups of pigs received an intramyocardial injection of either phVEGF165 (n = 6) or pCMVbeta (n = 7) 3 to 4 weeks after implantation of an ameroid constrictor around the left circumflex coronary artery. The injections caused no change in heart rate or blood pressure, and no ventricular arrhythmias or histologic evidence of inflammation. VEGF protein was detected by Western blot in VEGF-treated animals, with the strongest bands closest to the injection site. Plasma VEGF concentration (ELISA) increased from 3+/-2 to 27+/-13 pg/ml (p = 0.035) by day 4 after treatment. No increase in VEGF protein was noted in pCMVbeta-treated animals whereas these did stain positive for beta-Gal. Resting myocardial blood flow (colored microspheres) was significantly reduced in the ischemic versus nonischemic territory in control animals (1.07+/-0.05 versus 1.32+/-0.05; p < 0.05) but not VEGF-treated pigs (1.32+/-0.24 versus 1.13+/-0.12; p = NS). Maximal vasodilatation with adenosine significantly increased flow to the ischemic region in VEGF-treated pigs (2.16+/-0.57 versus 1.32+/-0.24; p < 0.05) but not controls (1.31+/-0.05 versus 1.17+/-0.06;p = NS). Collateral filling of the occluded circumflex artery improved in five of six VEGF-treated pigs (mean change in Rentrop score, +1.5). We conclude that direct intramyocardial transfection phVEGF165 is safe and capable of producing sufficient VEGF protein to enhance collateral formation and myocardial perfusion. This approach may offer an alternative therapy for patients with intractable myocardial ischemia not amenable to PTCA or CABG.
Movement Artefact Resistant Photoplethysmographic Probe (2014)

Photoplethysmogram (PPG) is an exceptionally informative diagnostic tool, but the signal registered by wearable devices usually is corrupted, because the probe is affected by the external force and moves with respect to the point of the measurement during the physical activities of the human. This problem is solved usually applying some techniques for the PPG extraction from the corrupted (noisy) signal. The authors of this paper are suggesting the solutions, which prevent corruption of PPG instead of noise filtering or other PPG extraction from corrupted signal techniques. One of such solutions is described in this paper, by proposing special photoplethysmographic signal probe (PPGP). This device emits the amplitude modulated light beam with special code sequence, which like Barker code, can be easily recognized using correlation method. The experimental evaluation of proposed method showed, that even in case of a very high noise level SNR=0dB correlation between reference PPG and photoplethysmogram got using proposed method is remarkable, reaching corr = 0.985, while standard deviation of error is small and equal std(err)=0.33. DOI: http://dx.doi.org/10.5755/j01.eee.20.3.6681