gebhart/patrepeval huggingface dataset:
Mucosal Immunization with a Novel Nanoemulsion-Based Recombinant Anthrax Protective Antigen Vaccine Protects againstBacillus anthracisSpore Challenge (2007)

The currently available commercial human anthrax vaccine requires multiple injections for efficacy and has side effects due to its alum adjuvant. These factors limit its utility when immunizing exposed populations in emergent situations. We evaluated a novel mucosal adjuvant that consists of a nontoxic, water-in-oil nanoemulsion (NE). This material does not contain a proinflammatory component but penetrates mucosal surfaces to load antigens into dendritic cells. Mice and guinea pigs were intranasally immunized with recombinant Bacillus anthracis protective antigen (rPA) mixed in NE as an adjuvant. rPA-NE immunization was effective in inducing both serum anti-PA immunoglobulin G (IgG) and bronchial anti-PA IgA and IgG antibodies after either one or two mucosal administrations. Serum anti-PA IgG2a and IgG2b antibodies and PA-specific cytokine induction after immunization indicate a Th1-polarized immune response. rPA-NE immunization also produced high titers of lethal-toxin-neutralizing serum antibodies in both mice and guinea pigs. Guinea pigs nasally immunized with rPA-NE vaccine were protected against an intradermal challenge with approximately 1,000 times the 50% lethal dose ( approximately 1,000x LD(50)) of B. anthracis Ames strain spores (1.38 x 10(3) spores), which killed control animals within 96 h. Nasal immunization also resulted in 70% and 40% survival rates against intranasal challenge with 10x LD(50) and 100x LD(50) (1.2 x 10(6) and 1.2 x 10(7)) Ames strain spores. Our results indicate that NE can effectively adjuvant rPA for intranasal immunization. This potentially could lead to a needle-free anthrax vaccine requiring fewer doses and having fewer side effects than the currently available human vaccine.
2-Oxoacid dehydrogenase multienzyme complexes in the halophilic Archaea? Gene sequences and protein structural predictions The GenBank accession number for the sequence reported in this paper is AF068743. (2000)

All Archaea catalyse the conversion of pyruvate to acetyl-CoA via a simple pyruvate oxidoreductase. This is in contrast to the Eukarya and most aerobic bacteria, which use the pyruvate dehydrogenase multienzyme complex [PDHC], consisting of multiple copies of three component enzymes: E1 (pyruvate decarboxylase), E2 (lipoate acetyl-transferase) and E3 (dihydrolipoamide dehydrogenase, DHLipDH). Until now no PDHC activity has been found in the Archaea, although DHLipDH has been discovered in the extremely halophilic Archaea and its gene sequence has been determined. In this paper, the discovery and sequencing of an operon containing the DHLipDH gene in the halophilic archaeon Haloferax volcanii are reported. Upstream of the DHLipDH gene are 3 ORFs which show highest sequence identities with the E1alpha, E1beta and E2 genes of the PDHC from gram-positive organisms. Structural predictions of the proposed protein product of the E2 gene show a domain structure characteristic of the E2 component in PDHCs, and catalytically important residues, including the lysine to which the lipoic acid cofactor is covalently bound, are conserved. Northern analyses indicate the transcription of the whole operon, but no PDHC enzymic activity could be detected in cell extracts. The presence in the E2 gene of an insertion (equivalent to approximately 100 aa) not found in bacterial or eukaryal E2 proteins, might be predicted to prevent multienzyme complex assembly. This is the first detailed report of the genes for a putative 2-oxoacid dehydrogenase complex in the Archaea, and the evolutionary and metabolic consequences of these findings are discussed.
Efficacy of Ibuprofen‐Hydrocodone for the Treatment of Postoperative Pain After Periodontal Surgery (2004)

The findings suggest that a combination analgesic preparation of ibuprofen (400 mg) with hydrocodone (5 mg) results in better pain control compared to ibuprofen used alone.
Geometric quantum gates that are robust against stochastic control errors (2005)

The realistic application of geometric quantum computation is crucially dependent on an unproved robustness conjecture, claiming that geometric quantum gates are more resilient against random noise than dynamic gates. We propose a suitable model that allows a direct and fair comparison between geometrical and dynamical operations. In the presence of stochastic control errors we find that the maximum of gate fidelity corresponds to quantum gates with a vanishing dynamical phase. This is a clear evidence for the robustness of nonadiabatic geometric quantum computation. The predictions here presented can be experimentally tested in almost all of the already existing quantum computer candidates.
T and B cell epitope mapping of SM23, an integral membrane protein of Schistosoma mansoni (1992)

SM23 is an integral membrane protein of the blood-vessel dwelling parasitic worm Schistosoma mansoni. This protein has been detected with antibodies in all stages of the parasite found in the human host, notably the lung stage, and therefore is of interest as a vaccine candidate. In addition SM23 has been shown to be a member of a proposed new superfamily of membrane proteins whose structures do not conform to the previously known classifications. To date there are 13 members including ME491 (CD63, Pltgp40), CD9 (p23), TAPA-1, CD37, CD53, MRC OX-44, CO-029, MRP-1, L6, the gene product of TI-1, the target of mAb AD-1, SM23, and SJ23 (the Schistosoma japonicum homologue). Most of these molecules except for those in the two blood vessel-dwelling parasites are found in membranes of hemopoietic and/or malignant cells and all have unknown function. In this study we used recombinantly expressed full-length and partial molecules as well as synthesized peptides to map T cell and B cell epitopes of SM23. The two predicted external hydrophilic domains were found to be highly immunogenic and contained several B cell epitopes. There were at least four T cell epitopes in the large hydrophilic domain. One segment of 23 amino acids contained both a T cell and B cell epitope as well as the putative glycosylation site. This particular segment was recognized by immune sera and cells of every mouse strain tested. The elucidation of these epitopes demonstrates the immunogenic nature of this molecule and raises questions as to the role of SM23 in the host/parasite relationship.
Impact of ustekinumab on health‐related quality of life and sexual difficulties associated with psoriasis: results from two phase III clinical trials (2011)

Ustekinumab treatment is associated with significant improvement in HRQoL and sexual difficulties due to psoriasis.
Real-time video sequences matching using the spatio-temporal fingerprint (2010)

With the growing popularity of digital multimedia distributed over broadband channels and networks copyright owners are requesting broadcasted content monitoring to keep their rights. A special need for multimedia monitoring is coming from advertisement submitters who would like to know how exactly their commercials are broadcasted and which categories of beholders are addressed. The basic unit needed for automated broadcast monitoring and its content evaluation is a robust and fast-enough video matching method. This paper proposes a method that can be used for matching of video streams or fast seeking of a short video sequence in a long video stream. According to real-time run requirements, the developed method is based on the brightness profile processing of significant image regions. Output from the algorithm is a one dimensional time-discretized vector of video sequence fingerprints that contains information about temporal and spatial video content. These fingerprints are appropriate to be stored in databases because of their significantly lower size in comparison to the source video. Having analyzed real video data, we show that reliable video sequence matching using introduced method can be achieved by using a video fingerprint data flow of 32 bytes per second of video stream. According to the robustness of proposed method this rate can be changed. This feature is very important for image processing systems with reduced data transfer bandwidth or low storage capacity, e.g. for the purpose of video-based TV channels rating measurement.
Unconventional method based on circular dichroism to detect peanut DNA in food by means of a PNA probe and a cyanine dye (2005)

In this paper we report an innovative and unconventional method based on circular dichroism for the identification of peanut DNA in food, which can be detected after PCR amplification at the nanomolar level by using an achiral PNA probe complementary to a tract of the peanut Ara h 2 gene and an achiral 3,3'-diethylthiadicarbocyanine dye [DiSC(2)(5)]. Peanuts are one of the most common causes of severe allergic reactions to foods and are particularly dangerous when they are "hidden" (undeclared) in food. For better protection of consumers, detection methods are required to specifically detect the presence of hidden allergens in a wide variety of food items. Alternative to the detection of the proteins is the determination of species-specific DNA, which is more resistant to technological treatments. PNAs are very specific probes able to recognize DNA sequences with high affinity and evidence for the binding can be obtained by using the DiSC(2)(5) dye, which aggregates onto the PNA-DNA duplex giving rise to a characteristic visibile band at 540 nm. Because the PNA-DNA duplex is in a right-handed helical conformation, the aggregation of the dye to the duplex gives also rise to a strong CD signal in the 500-600 nm region with a strong exciton coupling due to the formation of multimeric species, since the handedness of the helix is transferred to the dye aggregate. The dye does not interact with the free single-stranded DNA and although aggregating on the achiral PNA, this interaction is obviously not detectable by circular dichroism. Thus, only the formation of the PNA-DNA duplex, which takes place only upon specific Watson-Crick hydrogen binding between the PNA and the DNA bases, is detected, ensuring a very high specificity and sensitivity. The method has been optimized in a model system by using a synthetic oligonucleotide complementary to the PNA probe, showing that the intensity of the signal is linearly related to the amount of the DNA. The optimized method has been applied to the identification and quantitation of DNA extracted and amplified by PCR from peanuts and from peanut-containing foods, allowing for a very sensitive detection at a very low level (few pmol).
Nature of the iron-ligand bond in ferrous low spin hemoproteins studied by resonance Raman scattering (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNature of the iron-ligand bond in ferrous low spin hemoproteins studied by resonance Raman scatteringT. Kitagawa, Y. Kyogoku, T. Iizuka, and M. I. SaitoCite this: J. Am. Chem. Soc. 1976, 98, 17, 5169–5173Publication Date (Print):August 1, 1976Publication History Published online1 May 2002Published inissue 1 August 1976https://pubs.acs.org/doi/10.1021/ja00433a019https://doi.org/10.1021/ja00433a019research-articleACS PublicationsRequest reuse permissionsArticle Views360Altmetric-Citations127LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Overland Flow on Converging and Diverging Surfaces: Approximate Analytical Solutions (1984)

Approximate analytic solutions to overland flow on a diverging conical surface and a converging inverted conical surface are developed. The magnitude of error in these solutions is determined by undertaking a comparison between it and the solution given initially by an asymptotic expansion and then a numerical scheme.
Expression of the MyoD1 Muscle Determination Gene Defines Differentiation Capability but Not Tumorigenicity of Human Rhabdomyosarcomas (1989)

Several human rhabdomyosarcoma cell lines, cultured primary tumor explants, and biopsies of tumor and normal skeletal muscle tissue expressed a 2.0-kilobase transcript that hybridized to the mouse muscle determination gene MyoD1. This transcript was found in tumor cell lines and primary explants that developed multinucleated myotubes but was absent in Wilms' tumors or cell lines and primary explants that developed multinucleated myotubes but was absent in Wilms' tumors or cell lines derived from other mesenchymal tumor cell types. Expression of the human homolog of MyoD1 therefore can define a tumor as a rhabdomyosarcoma. Transfection of the mouse MyoD1 gene into the human rhabdomyosarcoma cell line RD increased the ability of the tumor cells to differentiate into multinucleated myotubes and enhanced myosin heavy-chain gene expression but did not decrease tumorigenicity in nude mice.
Protein Purification Techniques (2001)

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
[Asn31(N-beta-D-Glucopyranosyl)]hMOG(30-50): an antigen to identify anti-hMOG peptide antibodies in multiple sclerosis patients. (1998)

*
Classification of vestibular symptoms: Towards an international classification of vestibular disorders (2009)

Orthostatic dizziness * Other triggered dizziness 3. Vestibulo-
Treatment of Depression in Chronic Cocaine and Phencyclidine Abuse With Desipramine (1986)

An open field trial was conducted comparing desipramine and an active placebo in separate populations of chronic cocaine and phencyclidine (PCP) abusers, who discontinued their abuse. Subjects who received desipramine showed a decrease in depressive symptoms after a 20-40 day period regardless of whether they abused PCP or cocaine.
Multi-modal tracking of faces for video communications (2002)

Visual processes to detect and track faces for video compression and transmission. The system is based on an architecture in which a supervisor selects and activates visual processes in cyclic manner. Control of visual processes is made possible by a confidence factor which accompanies each observation. Fusion of results into a unified estimation for tracking is made possible by estimating a covariance matrix with each observation. Visual processes for face tracking are described using blink detection, normalised color histogram matching, and cross correlation (SSD and NCC). Ensembles of visual processes are organised into processing states so as to provide robust tracking. Transition between states is determined by events detected by processes. The result of face detection is fed into recursive estimator (Kalman filter). The output from the estimator drives a PD controller for a pan/tilt/zoom camera. The resulting system provides robust and precise tracking which operates continuously at approximately 20 images per second on a 150 megahertz computer workstation.
Blind Separation and Dereverberation of Speech Mixtures by Joint Optimization (2010)

This paper proposes a method for performing blind source separation (BSS) and blind dereverberation (BD) at the same time for speech mixtures. In most previous studies, BSS and BD have been investigated separately. The separation performance of conventional BSS methods deteriorates as the reverberation time increases while many existing BD methods rely on the assumption that there is only one sound source in a room. Therefore, it has been difficult to perform both BSS and BD when the reverberation time is long. The proposed method uses a network, in which dereverberation and separation networks are connected in tandem, to estimate source signals. The parameters for the dereverberation network (prediction matrices) and those for the separation network (separation matrices) are jointly optimized. This enables a BD process to take a BSS process into account. The prediction and separation matrices are alternately optimized with each depending on the other; hence, we call the proposed method the conditional separation and dereverberation (CSD) method. Comprehensive evaluation results are reported, where all the speech materials contained in the complete test set of the TIMIT corpus are used. The CSD method improves the signal-to-interference ratio by an average of about 4 dB over the conventional frequency-domain BSS approach for reverberation times of 0.3 and 0.5 s. The direct-to-reverberation ratio is also improved by about 10 dB.
Capacitive Sensing of Electrocardiographic Potential Through Cloth From the Dorsal Surface of the Body in a Supine Position: A Preliminary Study (2007)

A method for obtaining electrocardiographic potential through thin cloth inserted between the measuring electrodes and the skin of a subject's dorsal surface when lying supine has been proposed. The method is based on capacitive coupling involving the electrode, the cloth, and the skin. Examination of a pilot device which employed the method revealed the following: (1) In spite of the gain attenuation in the high frequency region, the proposed method was considered useful for monitoring electrogardiogram (ECG) for nondiagnostic purpose. (2) The method was able to yield a stable ECG from a subject at rest for at least 7 h, and there was no significant adverse effect of long-term measurement on the quality of the signal obtained. (3) Electrode area was the factor that had most influence on the signal, compared with other factors such as cloth thickness and coupling pressure, but could be reduced to 10 cm2 for heart rate detection. (4) Input capacitance of the device was assumed to be the dominant factor for the gain attenuation in the high frequency region, and should be reduced with a view to diagnostic use. Although there is still room for improvement in terms of practical use, the proposed method appears promising for application to bedding as a noninvasive and awareness-free method for ECG monitoring.
Lymphoid Follicle-Dense Mucosa at the Terminal Rectum Is the Principal Site of Colonization of Enterohemorrhagic Escherichia coli O157:H7 in the Bovine Host (2003)

Escherichia coli O157:H7 causes bloody diarrhea and potentially fatal systemic sequelae in humans. Cattle are most frequently identified as the primary source of infection, and E. coli O157:H7 generally colonizes the gastrointestinal tracts of cattle without causing disease. In this study, persistence and tropism were assessed for four different E. coli O157:H7 strains. Experimentally infected calves shed the organism for at least 14 days prior to necropsy. For the majority of these animals, as well as for a naturally colonized animal obtained from a commercial beef farm, the highest numbers of E. coli O157:H7 were found in the feces, with negative or significantly lower levels detected in lumen contents taken from the gastrointestinal tract. Detailed examination demonstrated that in these individuals the majority of tissue-associated bacteria were adherent to mucosal epithelium within a defined region extending up to 5 cm proximally from the recto-anal junction. The tissue targeted by E. coli O157:H7 was characterized by a high density of lymphoid follicles. Microcolonies of the bacterium were readily detected on the epithelium of this region by immunofluorescence microscopy. As a consequence of this specific distribution, E. coli O157:H7 was present predominantly on the surface of the fecal stool. In contrast, other E. coli serotypes were present at consistent levels throughout the large intestine and were equally distributed in the stool. This is a novel tropism that may enhance dissemination both between animals and from animals to humans. The accessibility of this site may facilitate simple intervention strategies.
Phase I/II clinical radioimmunotherapy with an iodine-131-labeled anti-carcinoembryonic antigen murine monoclonal antibody IgG. (1997)

These results suggest that prior chemotherapy or external beam radiation is an important risk factor for the development of hematological toxicity in radioimmunotherapy and that higher radiation doses may be delivered to tumors of patients without prior therapy compromising the bone marrow reserve. The different and, in the individual cases, unpredictable clearance rates suggest the necessity of dosimetry-based treatment planning rather than mCi/m2 dosing. Small tumors seem to be more suitable for radioimmunotherapy because of their favorable dosimetry, but to achieve better therapeutic results in patients with bulky disease, the application of higher, potentially myeloablative doses is indicated.