gebhart/patrepeval huggingface dataset:
Pulmonary Vascular Effects of Inhaled Nitric Oxide and Oxygen Tension in Bronchopulmonary Dysplasia (2004)

Pulmonary hypertension contributes significantly to morbidity and mortality in bronchopulmonary dysplasia (BPD), but little is known about the relative contribution of arterial tone, structural remodeling, and vessel density to pulmonary hypertension, especially in older patients. To determine the role of high pulmonary vascular tone in pulmonary hypertension, we studied the acute effects of oxygen tension, inhaled nitric oxide (iNO), and calcium channel blockers (CCB) in 10 patients with BPD who underwent cardiac catheterization for evaluation of pulmonary hypertension. During normoxic conditions, mean pulmonary arterial pressure (PAP) and pulmonary to systemic vascular resistance ratio (PVR/SVR) were 34 +/- 3 mm Hg and 0.42 +/- 0.07, respectively. In response to hypoxia, PAP and PVR/SVR increased by 50 +/- 8% and 82 +/- 14%, respectively (p < 0.01). Hyperoxia decreased PVR/SVR by 28 +/- 9% (p = 0.05). The addition of iNO treatment (20-40 ppm) to hyperoxia decreased PAP and PVR/SVR by 29 +/- 5% (p < 0.01) and 45 +/- 6% (p < 0.05) from baseline values, respectively, achieving near normal values. CCB did not alter PAP or PVR/SVR from baseline values. We conclude that hyperoxia plus iNO causes marked pulmonary vasodilatation in older patients with BPD, suggesting that heightened pulmonary vascular tone contributes to pulmonary vascular disease in BPD.
Evolving behaviors for a swarm of unmanned air vehicles (2005)

We have previously reported on a project involving the control of a swarm of unmanned air vehicles (UAVs) carrying out search or search-and-destroy missions. We developed and tested (in simulation) a number of strategies for swarm control, and proposed systematic evaluation techniques and performance metrics. In this paper we report some additional results in which we evolved some of the swarm control parameters using a genetic algorithm (GA). While the improvements were modest, the results show how evolutionary computing algorithms can be used to facilitate the design of swarm control algorithms.
TRICOM Vector Based Cancer Vaccines (2005)

For the immune system to mount an effective antitumor T-cell response, an adequate number of T-cells specific for the antigens expressed by the malignancy must be activated [1]. Since most antigens expressed by tumors are "self"-antigens, tumor antigens often lack endogenous immunogenicity and thus do not sufficiently activate T-cells to levels that can mediate tumor eradication. In addition, virtually all solid tumor cells lack the costimulatory molecules necessary to activate tumor-specific T-cells. Approaches that stimulate immune responses to these tumor antigens have the potential to alter this poor responsiveness. This theory has promoted the use of active immunotherapy to generate immune responses against tumor-associated antigens (TAAs) for the treatment of cancer. As one such vaccine strategy, we have utilized poxviruses as delivery vehicles for TAAs in combination with T-cell costimulatory molecules. Initial studies have demonstrated that the insertion of costimulatory molecule trangenes into viral vectors, along with a TAA transgene, greatly enhances the immune response to the antigen. Using this approach, a TRIad of COstimulatory Molecules (TRICOM; B7-1, ICAM-1 and LFA-3) has been shown to enhance T-cell responses to TAAs to levels far greater than any one or two of the costimulatory molecules in combination. In this article, preclinical findings and recent clinical applications of TRICOM-based vaccines as a cancer immunotherapy are reviewed.
Mac OS X Unleashed (2001)

From the Publisher:
Mac OS X Unleashed is a complete guide and reference for Mac OS users. The power of OS X is largely in its underlying BSD implementation. For an average Mac user, experiencing a command line for the first time can be an unpleasant experience. This book will take the approach of demonstrating familiar actions and tools, and then provide the equivalent information within the BSD environment. For advanced users, this book will cover system administration ranging from advanced configuration of FTP servers, SSH tunneling, to installing secure E-mail and Web servers. The progression of the text is designed to move from simple using topics through advanced configuration - making it easier for beginners and experienced users to find what they're looking for.

In short, Mac OS books have never had to deal with issues beyond the Macintosh GUI. This book will bridge the gap between the user interface and the BSD subsystem in a way that is accessible to users who are unfamiliar with UNIX and other multi-user systems.
Tyrphostin AG490, a tyrosine kinase inhibitor, blocks actively induced experimental autoimmune encephalomyelitis (1998)

Migration of lymphocytes from blood into the brain is a critical event in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Previous observations made in our laboratory showed that protein tyrosine kinase inhibitors were able to block lymphocyte adhesion to brain endothelium and prevent the entry of encephalitogenic T cell lines into the brain of SJL/J mice. Here we show that systemic administration of the protein tyrosine kinase inhibitor, tyrphostin AG490, blocks the development of actively induced EAE in a dose-dependent manner. Administration of 1 mg of drug daily significantly decreased the severity of the disease, while 3 mg of AG490 daily totally blocked the disease in 62% of treated animals, and in those that developed the disease, paralysis was delayed and clinical score was significantly reduced. Blood leukocytes isolated from mice treated with tyrphostin AG490 were less adhesive on VCAM-1 and fibronectin, when compared with control animals. AG490 treatment had no effect on the proliferation by antigen-stimulated peripheral lymph nodes cells. Interestingly, cells obtained from draining lymph nodes in AG490-treated animals and stimulated with antigen secreted two times more IFN-gamma and four times more IL-10, when compared with control animals, whereas no difference was observed in TNF-alpha production. Our results suggest that tyrphostin AG490 may have therapeutic potential by blocking tyrosine kinase activities involved in key mechanisms leading to demyelinating diseases of the central nervous system.
Microdermabrasion (2005)

In general, microdermabrasion studies have been conducted in small groups of patients. Protocols, units, and settings have differed. There remains a major disparity between the popularity of microdermabrasion in the public sector and cohesive and comprehensive scientific data documenting the efficacy of the procedure.
Modulation of radiation response and tumor-induced angiogenesis after epidermal growth factor receptor inhibition by ZD1839 (Iressa). (2002)

ZD1839 ("Iressa") is an orally-active, selective epidermal growth factor receptor-tyrosinekinase inhibitor. We evaluated the antitumor activity of ZD1839 in combination with radiation in human squamous cell carcinomas (SCCs) of the head and neck. ZD1839 produced a dose-dependent inhibition of cellular proliferation in human SCCs grown in culture. Flow cytometry analysis of cell cycle progression confirmed the accumulation of cells in G(1) phase after exposure to ZD1839. Clonogenic analysis demonstrated that treatment of SCCs with ZD1839 reduced cell survival after radiation exposure. Flow cytometric analysis further demonstrated that treatment of SCCs with ZD1839 amplified radiation-induced apoptosis. Tumor xenograft studies confirmed that oral administration of ZD1839, or focal radiation, resulted in partial and transient tumor regression in both SCC-1 and SCC-6 xenografts. In contrast, profound tumor regression and regrowth delay was observed in mice treated with the combination of ZD1839 and radiation. To examine antiangiogenic effects, we studied the impact of ZD1839 on human umbilical vascular endothelial cells (HUVECs). In the presence of reconstituted Matrigel matrix, HUVECs established a capillary-like network structure (tube formation). Treatment with ZD1839 reduced the cell-to-cell interaction of HUVECs, resulting in disruption of tube formation. The effect of ZD1839 was further examined using an in vivo tumor xenograft model of angiogenesis (Matrigel plug) in athymic mice. Systemic treatment with ZD1839 significantly inhibited tumor-induced neovascularization across the Matrigel plug. Taken together, these results suggest that the antitumor activity of ZD1839 in combination with radiation appears to derive from not only proliferative growth inhibition (with associated cell cycle arrest and enhancement of radiation-induced apoptosis) but also from inhibition of tumor angiogenesis.
Physicochemical and toxicological characterization of a new generic iron sucrose preparation (2011)

Intravenous iron preparations are key components in the management of anaemia of various etiologies. These iron-carbohydrate complexes permit safe systemic delivery of iron, whilst protecting from the potential toxic effects of over-saturation. This in turn permits efficient haematopoiesis following erythropoietin administration. Since the rate of release of iron is dependent upon the structure of this iron-carbohydrate complex, it is essential to ensure that an intravenous iron preparation is well characterized and its properties documented. This report describes physicochemical and toxicological studies into a new iron sucrose generic preparation, "Iron Sucrose Azad (ISA)", using the original iron sucrose product as reference. It could be demonstrated that the specifications and physicochemical characteristics of ISA reflect those of the reference product. Furthermore, in a rat model previously shown to identify possible toxicological effects of "unsimilar" iron sucrose preparations, ISA was found to have the same properties as the reference product, with both being well tolerated.
Antioxidative activity and phenolic composition of pilot‐plant and commercial extracts of sage and rosemary (1996)

Abstract Eight sage ( Salvia officinalis ) and twenty‐four rosemary ( Rosmarinus officinalis ) extracts, originating from pilot‐plant or commercial sources, had different antioxidative activities as measured by accelerated autoxidation of methyl linoleate. Twenty‐seven compounds were characterized in the Labiatae family extracts by high‐performance liquid chromatography (HPLC) coupled with mass spectrometry, equipped with an atmospheric pressure chemical ionization interface, and by HPLC coupled with a photodiode array spectrophotometer. Twenty‐two compounds were identified, including phenolic acids, carnosol derivatives, and flavonoids. The extracts showed great variation in their HPLC profiles, and no correlation was apparent between their antioxidative efficiency and their composition, in twenty specific phenols. Data indicated that the most effective compounds were carnosol, rosmarinic acid, and carnosic acid, followed by caffeic acid, rosmanol, rosmadial, genkwanin, and cirsimaritin.
A three-step camera calibration method (1997)

Camera calibration is a crucial problem for many industrial applications that incorporate visual sensing. In this paper, we compute the intrinsic and extrinsic calibration parameters in three steps. In the first step, the calibration parameters are approximated using the linear least-squares method. In the second step, we develop two alternative formulations to obtain an optimal rotation matrix from the calibration parameters computed in the first step. Further optimization of translational and perspective transformations is then performed based on the optimized rotation matrix. In the third step, a nonlinear optimization is performed to handle lens distortion. The solution of the nonlinear system not only minimizes the perspective transformation relations between the image points and the corresponding world coordinates, but also satisfies the orthonormality constraints on the rotational transformation. To assess the performance of our proposed method, the Euclidean norm of the error matrix between the calculated and the original 4/spl times/4 homogeneous transformation matrices is used as a basis for comparison with existing methods. Simulation results from applying the method show significant improvements both before and after the nonlinear optimization step.
Immunostimulatory DNA for Asthma (2003)

Keywords: interleukin, IL; immunostimulatory sequence-ODN, ISS-ODN; natural killer, NK; oligodeoxynucleotides, ODN; pathogen-associated molecular patterns, PAMP; pattern recognition receptors, PRRs; transforming-growth factor-β1, TGF-β1; T helper, TH; toll-like receptor 9, TLR9; T regulatory, Tr
Study of the Substrate‐Binding Properties of Bovine Liver Adenosine Kinase and Inhibition by Fluorescent Nucleoside Analogues (1997)

Adenosine kinase (AK) catalyzes the phosphorylation of adenosine to AMP with ATP as phosphate donor. Intrinsic fluorescence of bovine liver AK was shown previously to be a sensitive probe to quantify the binding of substrates to the enzyme [Elaloui, A., Divita, G., Maury, G., Imbach, J.-L. & Goody, R. S. (1994) Eur. J Biochem. 221, 839-846]. AK contains two catalytic, sites: a high-affinity site, which binds adenosine and AMP selectively; and a site for ATP and ADP. In the present work, these two sites were characterized by combining the quenching of protein fluorescence induced by the binding of the ligands and the fluorescence enhancement observed upon binding of the N-methylanthraniloyl-derivated nucleotides or adenosine. A new fluorescent analog of adenosine, 5'-N-methylanthraniloyl-adenosine, was synthesized and shown to bind selectively to the high-affinity adenosine-binding site with an affinity similar to that of adenosine (Kd 1 microM). In contrast, 2'(3')-N-methylanthraniloyl derivatives of ATP, adenosine (5')tetraphospho(5')adenosine (Ap4A), and adenosine (5')pentaphospho(5')adenosine (Ap5A), bind to the enzyme at the ATP site. Methylantraniloyl derivatives of ATP and adenosine were used as tools for selective characterization of a series of adenosine analogues. The bisubstrate inhibitors Ap4A and Ap5A bind to the ATP site with high affinity and apparently not to the adenosine site, thus acting more as ATP analogues than true bisubstrate ligands. The binding properties of a series of adenosine analogues were strongly dependent on the structural modifications on adenosine. The analogues modified at positions 2' or 3' show similar affinities for AK as that of adenosine, whereas adenosine analogues modified at the base present a relatively low affinity for the enzyme.
Clinical observation of gemcitabine combined vinorelbine×8 regimen versus vinorelbine combined vinorelbine×4 follow G or N ×4 regimen as chemotherapy for anthracycline and taxane resistant metastatic breast cancer (2011)

Objective To compare the efficacy and toxicities of gemcitabine combined vinorelbine(GN) 8 regimen and gemcitabine combined vinorelbine(GN) 4 follow G or N 4 regimen as chemotherapy for anthracycline-and taxane-resistant metastatic breast cancer(MBC).Methods 58 patients were treated by GN or GN 4-G or N regimen.Overall response rate(ORR)and 1-year survival of two groups were assessed after treatment.Results CR was 6.5%(2/31)and PR was 29.0%(9/31)in GN group.2 patients(6.9%)had a complete response(CR)and 8 patients(27.6%)had a partial response(PR)in GN×4-G or N group.No statistically significant difference was observed in terms of ORR(35.5% vs.34.51%,P= 0.935),or 1-year survival rate(67.7% vs.65.5%,P=0.586) of the two groups.No patients died in correlated with the two regimens.Myelosuppression and gastrointestinaltract reaction were the most common toxicities.Conclusions GP and GN 4-G or N regimens are both well effective for patients with anthracycline-and taxane-resistant MBC.Drug-related toxicities are tolerable.Two regimens can be considered as salvage regimens for those patients.
Word lengths are optimized for efficient communication (2011)

We demonstrate a substantial improvement on one of the most celebrated empirical laws in the study of language, Zipf's 75-y-old theory that word length is primarily determined by frequency of use. In accord with rational theories of communication, we show across 10 languages that average information content is a much better predictor of word length than frequency. This indicates that human lexicons are efficiently structured for communication by taking into account interword statistical dependencies. Lexical systems result from an optimization of communicative pressures, coding meanings efficiently given the complex statistics of natural language use.
The HspA2 protein localizes in nucleoli and centrosomes of heat shocked cancer cells (2008)

The human HSPA2 gene, which belongs to the HSP70 family of heat shock genes, is a counterpart of rodent testis-specific HspA2 gene. Rodent genes are expressed mainly in pachytene spermatocytes, while transcripts of human HSPA2 gene have been detected in various normal somatic tissues, albeit translation of the messenger RNA into corresponding protein has not been yet unambiguously demonstrated, except for several cancer cell lines. The aim of our work, a first step in search for HspA2 function in cancer cells, was to establish its intracellular localization at physiological temperature and during heat shock. First, we used qRT-PCR and a highly specific antibody to select cell lines with the highest expression of the HspA2 protein, which turned out to be A549 and NCI-H1299 lines originating from non-small cell lung carcinoma (NSCLC). Significant expression of the HspA2 was also detected by immunohistochemistry in primary NSCLC specimens. Intracellular localization of the HspA2 was studied using both the specific anti-HspA2 polyclonal antibody and transfection of cells with fusion proteins HspA2-EGFP and mRFP-HspA2. We found that, at physiological temperature, the HspA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli. Moreover, we demonstrate that in heat-shocked cells HspA2 accumulated in centrosomes. Our results suggest that the HspA2, like Hsp70 protein, can be involved in protecting nucleoli and centrosomes integrity in cancer cells subjected to heat shock and, possibly, other cellular stressors.
Pancreatic cancer (2000)

The incidence of pancreatic adenocarcinoma in the USA remains stable. Advances in management are confined to more accurate preoperative diagnosis and staging, and decreased operative mortality. Long-term survival in the absence of resection remains poor.
The Graph SLAM Algorithm with Applications to Large-Scale Mapping of Urban Structures (2006)

This article presents GraphSLAM, a unifying algorithm for the offline SLAM problem. GraphSLAM is closely related to a recent sequence of research papers on applying optimization techniques to SLAM problems. It transforms the SLAM posterior into a graphical network, representing the log-likelihood of the data. It then reduces this graph using variable elimination techniques, arriving at a lower-dimensional problems that is then solved using conventional optimization techniques. As a result, GraphSLAM can generate maps with 108 or more features. The paper discusses a greedy algorithm for data association, and presents results for SLAM in urban environments with occasional GPS measurements.
Experimental Development and Evaluation of Pneumatic Powered-Lift Super-STOL Aircraft (2006)

The powered-lift Channel Wing concept has been combined with pneumatic Circulation Control aerodynamic and propulsive technology to generate a Pneumatic Channel Wing (PCW) configuration intended to have Super-STOL or VSTOL capability while eliminating many of the operational problem areas of the original Channel Wing vehicle. Wind-tunnel development and evaluations of a PCW powered model conducted at Georgia Tech Research Institute (GTRI) have shown substantial lift capabilities for the blown configuration (CL values of 10 to 11). Variation in blowing of the channel was shown to be more efficient than variation in propeller thrust in terms of lift generation. Also revealed was the ability to operate unstalled at very high angles of attack of 40 deg - 45 deg, or to achieve very high lift at much lower angle of attack to increase visibility and controllability. In order to provide greater flexibility in Super-STOL takeoffs and landings, the blown model also displayed the ability to interchange thrust and drag by varying blowing without any moving parts. A preliminary design study of this pneumatic vehicle based on the two technologies integrated into a simple Pneumatic Channel Wing configuration showed very strong Super-STOL potential. This paper presents these experimental results, discusses variations in the configuration geometry under development, and addresses additional considerations to extend this integrated technology to advanced design studies of PCW-type vehicles.
HMG-CoA Reductase Inhibitors Regulate Inflammatory Transcription Factors in Human Endothelial and Vascular Smooth Muscle Cells (2003)

HMG-CoA reductase inhibitors downregulate the activation of transcription factors NF-kappaB, AP-1, and hypoxia-inducible factor-1alpha. These findings support the concept that statins have antiinflammatory and antiproliferative effects that are relevant in the treatment of atherosclerotic diseases.
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli (2005)

Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural protein sources rarely meet the requirements for quantity, ease of isolation or price and hence recombinant technology is often the method of choice. Recombinant cell factories are constantly employed for the production of protein preparations bound for downstream purification and processing. Eschericia coli is a frequently used host, since it facilitates protein expression by its relative simplicity, its inexpensive and fast high density cultivation, the well known genetics and the large number of compatible molecular tools available. In spite of all these qualities, expression of recombinant proteins with E. coli as the host often results in insoluble and/or nonfunctional proteins. Here we review new approaches to overcome these obstacles by strategies that focus on either controlled expression of target protein in an unmodified form or by applying modifications using expressivity and solubility tags.
Direct Regulation of ZAP-70 by SHP-1 in T Cell Antigen Receptor Signaling (1996)

The threshold at which antigen triggers lymphocyte activation is set by the enzymes that regulate tyrosine phosphorylation. Upon T cell activation, the protein tyrosine phosphatase SHP-1 was found to bind to the protein tyrosine kinase ZAP-70. This interaction resulted in an increase in SHP-1 phosphatase activity and a decrease in ZAP-70 kinase activity. Expression of a dominant negative mutant of SHP-1 in T cells increased the sensitivity of the antigen receptor. Thus, SHP-1 functions as a negative regulator of the T cell antigen receptor and in setting the threshold of activation.