gebhart/patrepeval huggingface dataset:
Advances in Virus-Like Particle Vaccines for Filoviruses (2011)

Ebola virus (EBOV) and Marburg virus (MARV) are among the deadliest human pathogens, with no vaccines or therapeutics available. Multiple vaccine platforms have been tested for efficacy as prophylactic pretreatments or therapeutics for prevention of filovirus hemorrhagic fever. Most successful vaccines are based on a virus-vectored approach expressing the protective glycoprotein (GP); protein-based subunit and DNA vaccines have been tested with moderate success. Virus-like particle (VLP) vaccines have realized promising results when tested in both rodents and nonhuman primates. VLPs rely on the natural properties of the viral matrix protein (VP) 40 to drive budding of filamentous particles that can also incorporate ≥ 1 other filovirus protein, including GP, VP24, and nucleoprotein (NP). Filovirus VLP vaccines have used particles containing 2 or 3 (GP and VP40, with or without NP) viral proteins generated in either mammalian or insect cells. Early studies successfully demonstrated efficacy of bivalent VLP vaccines in rodents; more recent studies have shown the ability of the VLP vaccines containing GP, NP, and VP40 to confer complete homologous protection against Ebola virus and Marburg virus in a prophylactic setting against in macaques. This review will discuss published work to date regarding development of the VLP vaccines for prevention of lethal filovirus hemorrhagic fever.
Bronchopulmonary dysplasia of the premature baby (1997)

Prematurely born infants who required assisted ventilation may develop chronic lung disease or bronchopulmonary dysplasia (BPD). The cells involved in the reparative process of the premature lung are not well defined. The repair of injured tissues is a highly standardized process and the most important cells are activated (modulated) fibroblasts (myofibroblasts). A key cytokine in controlling repair is transforming growth factor-β (TGF-β). To characterize the cells involved in the repair process of the premature lung, we employed immunocytochemical techniques and examined the lungs of 39 autopsied premature babies who had neonatal respiratory distress syndrome (RDS). All were treated in neonatal intensive care units and required mechanical ventilation and supplemental oxygen; all survived for at least 12 hours. Antibodies were employed against vimentin, α-smooth muscle (α-SM) actin, total muscle actin, desmin, MAC387, and TGF-β. Our study indicates that myofibroblasts are normally present along terminal airways in the developing lung. These cells increase in number some days after lung injury, form bundles of cells encircling terminal air spaces, and acquire desmin contractile filaments shortly thereafter. Myofibroblasts do not lose their contractile filaments with time, suggesting a conversion to smooth muscle metaplasia. The proliferation and migration of such myofibroblasts at sites of lung injury is associated with the presence of TGF-β. These findings suggest that myofibroblasts play an important role in premature lung repair. They may point the way to experimental and clinical trials that will identify drugs antagonistic to TGF-β (or other cytokines). Such antagonists may protect the neonates who are at high risk of developing BPD. Pediatr. Pulmonol. 1997;24:22–28. © 1997 Wiley-Liss, Inc.
Complex of Meningococcal Group B Polysaccharide and Type 2 Outer Membrane Protein Immunogenic in Man (1979)

A noncovalent complex of meningococcal group B polysaccharide and type 2 outer membrane protein has been characterized and its potential as a vaccine against group B meningococcal disease investigated. The polysaccharide component was found to have a partition coefficient, K(d), of 0.34 on Sepharose CL-4B in the presence of sodium deoxycholate. The protein consisted of four to five major proteins including the principal outer membrane protein. Hydrophobic binding between the protein and polysaccharide was demonstrated by gel filtration and isopycnic CsCl density gradient centrifugation and found to involve all of the proteins. After demonstrating safety and immunogenicity in animals, two lots of vaccine were tested in a total of eight volunteers. Two 120-mug doses were given subcutaneously at 0 and 5 wk. Mild local reactions occurred in all eight volunteers, but no systemic reactions were observed. 2 wk after the first dose, six of the volunteers had increased levels of bactericidal antibodies against both the group B polysaccharide and the outer membrane proteins. Antibody rises to the group B polysaccharide (mean 6-fold) were confirmed by passive hemagglutination assays and rises to the proteins (mean 10-fold) by a solid phase radioimmunoassay. The second dose resulted in little or no increase in antibody titers. Antibody titers declined over a period of 14 wk but mostly remained above preimmunization levels. Bactericidal antibodies with specificity for the group B polysaccharide were mostly of the immunoglobulin (Ig)M class, and were directed against a determinant associated only with high molecular weight polysaccharides. We conclude that both the group B polysaccharide and the outer membrane protein are immunogenic in man when presented as a complex and that the complex warrants further testing and development as a vaccine against group B meningococcal disease.
A niche for enhanced oil recovery in the 1990s (1992)

Aging oil fields and shrinking reserves are making new demands for enhanced oil recovery. New understanding of reservoir dynamics is enhancing the efficiency of the three main Enhanced Oil Recovery (EOR) methods: thermal, miscible and chemical recovery methods, describing which situations favor the techniques and which rule them out. 9 figs., 14 refs.
The Role of Melatonin in the Pathogenesis of Adolescent Idiopathic Scoliosis (1996)

Although melatonin deficiency may cause scoliosis in the chicken, this study suggests that it is not a mechanism in the pathogenesis of adolescent idiopathic scoliosis in humans.
Catalytic Asymmetric C−H Activation of Alkanes and Tetrahydrofuran (2000)

Rhodium carbenoids derived from methyl aryldiazoacetates are capable of effective catalytic asymmetric C−H activation of a range of alkanes and tetrahydrofuran by a C−H insertion mechanism. Dirhodium tetrakis(S-(N-dodecylbenzenesulfonyl)prolinate) (Rh2(S-DOSP)4) catalyzed decomposition of methyl aryldiazoacetates in the presence of alkanes results in intermolecular C−H insertions with good control of regioselectivity, diastereoselectivity, and enantioselectivity. The carbenoids derived from methyl aryldiazoacetates are considerably more chemoselective than carbenoids derived from diazoacetates. They strongly favor C−H insertions into secondary and tertiary sites. Formation of side products due to carbene dimerization is not a major problem with rhodium carbenoids derived from aryldiazoacetates.
PCA-based network Traffic anomaly detection (2016)

The use of a Traffic Matrix (TM) to describe the characteristics of a global network has attracted significant interest in network performance research. Due to the high dimensionality and sparsity of network traffic, Principal Component Analysis (PCA) has been successfully applied to TM analysis. PCA is one of the most common methods used in analysis of high-dimensional objects. This paper shows how to apply PCA to TM analysis and anomaly detection. The experiment results demonstrate that the PCA-based method can detect anomalies for both single and multiple nodes with high accuracy and efficiency.
Molecular cloning and characterization of Stevia rebaudiana UDP-glucosyltransferase. (2003)

We report here the cloning and characterization of a UDP-glucose flavonoid glucosyltransferase (srUFGT) in Stevia rebaudiana. The isolated cDNA was 1419 bp in length encoding 473 deduced amino acids with a predicted molecular mass of 53.2 kDa. The products of in vitro translation from an expression vector had anthocyanidins and steviol glucosyltransferase activity. Comparison of the activity of the recombinant UDP-glucosyltransferase toward a range of acceptor substrates suggests that it may participate in the synthesis of steviol glycosides. The results support the hypothesis that the flavonoid glucosyltransferases, which have a broad substrate specificity, may be not only involved in flavonoid glucosylation but also play a role in producing the water-soluble steviol-glycosides in S. rebaudiana.
Tools for privacy preserving distributed data mining (2002)

Privacy preserving mining of distributed data has numerous applications. Each application poses different constraints: What is meant by privacy, what are the desired results, how is the data distributed, what are the constraints on collaboration and cooperative computing, etc. We suggest that the solution to this is a toolkit of components that can be combined for specific privacy-preserving data mining applications. This paper presents some components of such a toolkit, and shows how they can be used to solve several privacy-preserving data mining problems.
Isolation and characterization of bovine factor IX (Christmas factor) (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation and characterization of bovine factor IX (Christmas factor)Kazuo Fujikawa, Arthur R. Thompson, Mark E. Legaz, Robert G. Meyer, and Earl W. DavieCite this: Biochemistry 1973, 12, 24, 4938–4945Publication Date (Print):November 1, 1973Publication History Published online1 May 2002Published inissue 1 November 1973https://doi.org/10.1021/bi00748a019RIGHTS & PERMISSIONSArticle Views150Altmetric-Citations156LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alertsclose Get e-Alerts
The Development of a Thermo-Oxidation Engine Oil Simulation Test (TEOST) (1993)

&lt;div class="htmlview paragraph"&gt;This paper concerns a bench test developed to simulate the effect of engine operating conditions on the oxidation and deposit-forming tendencies of engine oils. The so-called Thermo-oxidation Engine Oil Simulation Test (TEOST) is carried out under temperatures and other environmental conditions identified as being significant in the internal combustion engine. These parameters can be readily modified to reflect different aspects of deposit conditions and/or different forms of the mechanical design of reciprocating engines.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;The most important aspect of the TEOST is the separation of the oxidation process into the two aspects believed to be present in the engine, (1) the preparation of oxidation precursors in the so-called ‘Reactor’ representing the engine sump and other moderately heated areas of oil exposure, and (2) the ‘Depositor’ representing those areas of the engine where temperatures are such that the completion of the deposit-forming oxidation mechanism can be induced. Many variations of time, temperature, oil flow rate, cycling rate of oil temperature, and condition of oxidation precursor formation were investigated. These ultimately led to a test protocol showing particular promise regarding the evaluation of modern engine oils.&lt;/div&gt;
Impact of different intensities of intermittent theta burst stimulation on the cortical properties during TMS‐EEG and working memory performance (2017)

Intermittent theta burst stimulation (iTBS) is a noninvasive brain stimulation technique capable of increasing cortical excitability beyond the stimulation period. Due to the rapid induction of modulatory effects, prefrontal application of iTBS is gaining popularity as a therapeutic tool for psychiatric disorders such as depression. In an attempt to increase efficacy, higher than conventional intensities are currently being applied. The assumption that this increases neuromodulatory may be mechanistically false for iTBS. This study examined the influence of intensity on the neurophysiological and behavioural effects of iTBS in the prefrontal cortex. Sixteen healthy participants received iTBS over prefrontal cortex at either 50, 75 or 100% resting motor threshold in separate sessions. Single-pulse TMS and concurrent electroencephalography (EEG) was used to assess changes in cortical reactivity measured as TMS-evoked potentials and oscillations. The n-back task was used to assess changes in working memory performance. The data can be summarised as an inverse U-shape relationship between intensity and iTBS plastic effects, where 75% iTBS yielded the largest neurophysiological changes. Improvement in reaction time in the 3-back task was supported by the change in alpha power, however, comparison between conditions revealed no significant differences. The assumption that higher intensity results in greater neuromodulatory effects may be false, at least in healthy individuals, and should be carefully considered for clinical populations. Neurophysiological changes associated with working memory following iTBS suggest functional relevance. However, the effects of different intensities on behavioural performance remain elusive in the present healthy sample.
CXCR-4 (Fusin), a co-receptor for the type 1 human immunodeficiency virus (HIV-1), is expressed in the human brain in a variety of cell types, including microglia and neurons. (1997)

Entry of the type 1 human immunodeficiency virus into most cells requires the presence of the CD4 protein in combination with one of several recently described co-receptors. CXCR-4 (fusin) was the first identified, and it serves as co-receptor for T-cell-line tropic (T-tropic) HIV-1 isolates. To determine the expression of CXCR-4 in the brain, a major target of HIV pathology, we used immunohistochemistry and reverse transcriptase polymerase chain reaction with CXCR-4-specific antibodies and probes. We found that CXCR-4 was expressed in several cell types in brain, but notably in neurons and microglia, a finding that was replicated in tissue culture. The study of the expression of CXCR-4 in the brain, which may be one of many chemokine receptors in the central nervous system, may provide further insight into the interactions between brain cells, pathogens, and the immune system, and help understand the pathogenesis of HIV dementia.
1 alpha,25-dihydroxyvitamin D3 receptors in cultured rat osteoblast-like cells. Glucocorticoid treatment increases receptor content. (1983)

The direct actions of 1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) in target cells are initiated by the binding of hormone to specific receptor sites. The aims of this study were to characterize the receptor for 1,25(OH)2D3 in cultured rat osteoblast-like (OB) cells and to determine whether these receptors are regulated by either the culture cycle itself or glucocorticoid treatment. The 1,25(OH)2D3 receptor in rat OB cells exhibited the same apparent binding affinity (Kd = 0.1 nM) and sedimentation coefficient (3.2 S) as mouse OB cells and receptors in other target organs. However, the receptor concentration in rat OB cells was substantially lower than mouse OB cells (approximately 20%). The concentration of receptors in rat OB cells did not show a correlation with the rate of DNA synthesis and therefore did not exhibit an endogenous rhythm in receptor concentration as was previously seen in mouse OB cells. Also in contrast to mouse cells, where glucocorticoids caused a decrease in receptor level, dexamethasone induced a marked increase in receptor binding throughout the culture cycle. This increase was due to an increase in receptor number with no change in receptor affinity. The change was glucocorticoid-specific, dose-dependent with half-maximal stimulation occurring between 1.3 and 13 nM and exhibited a latent period of at least 4 h. The independence of receptor concentration from DNA synthesis rate was established by assessing receptors after stimulating cell proliferation with epidermal growth factor and inhibiting it with hydroxyurea. Neither treatment altered basal 1,25(OH)2D3 receptor concentration or prevented the marked increase in receptor levels elicited by dexamethasone. We conclude that although the biochemical characteristics of 1,25(OH)2D3 receptors are indistinguishable in rat and mouse OB cells, there are genuine species differences in the regulation of the receptor number as it relates to DNA synthesis rate and response to glucocorticoids.
Induction of 103-kDa gelatinase/type IV collagenase by acidic culture conditions in mouse metastatic melanoma cell lines. (1992)

Gelatinases/type IV collagenases have been shown to be involved in tumor invasion and metastasis. In this study, we examined the effect of culture medium pH on the secretion of the gelatinases from mouse B16 melanoma cell lines and human tumor cell lines using zymography analysis. The highly metastatic clone F10 of B16 melanoma did not secrete any gelatinase in neutral culture media (pH 7.1-7.3), whereas it secreted a high level of a 103-kDa gelatinase in an initial pH range of 5.4-6.1. The addition of an excess amount of glucose into a neutral culture medium also induced the gelatinase secretion from the cells by decreasing the medium pH during incubation. The extent of the acid-induced gelatinase secretion by the B16 melanoma cell lines was in the order of BL6 greater than F10 greater than F1 much greater than the parent B16 line, in good agreement with the order of their metastatic potentials. Two human cell lines (A549 and HT1080) secreted a higher level of a 90-kDa gelatinase at pH 6.8 compared with pH 7.3. The acid-induced gelatinase secretion from B16-F10 cells was blocked by cycloheximide, indicating that the enzyme induction was due to de novo synthesis. When in vitro tumor cell invasion was assayed in Boyden chambers, B16-F10 cells incubated in an acidic medium exerted a more active migration through type IV collagen gel than those in a neutral medium. These results suggest that the acidic environment formed around tumor tissues may be an important factor in invasion and metastasis of some types of tumors.
A Stabilizing Influence: CAL PDZ Inhibition Extends the Half‐Life of ΔF508‐CFTR (2010)

It pays to recycle! The most common mutation in cystic fibrosis (CF) impedes maturation and accelerates breakdown of the ion channel CFTR. A “stabilizer” has been characterized that blocks a PDZ domain responsible for CFTR degradation. The inhibitor iCAL36 extends the chloride channel's half-life in airway epithelial cells. It also complements the activity of a corrector of the maturation defect, thus suggesting the potential for combination CF therapies.
A quantitative immunohistochemical study of the expression of integrins by nerves in psoriatic and normal skin (1992)

Qualitative and quantitative assessment of integrin expression by dermal nerves was made by an avidin-biotin immunoperoxidase method on snap-frozen biopsies from affected psoriatic skin, and skin from normal control subjects with no history of skin disease. Nerves expressed alpha 1, alpha 2, alpha 3, alpha 6, beta 1 and beta 4 integrin subunits, and perineural sheaths in the mid-dermis also expressed these subunits, with the exception of alpha 2. There were more upper dermal nerve segments expressing alpha 1 integrin compared with other integrins both in controls and in psoriatic skin. The greater number of nerves expressing alpha 1 integrin compared with other integrins may be due to anatomical or functional differences between groups of nerves. There were significantly more nerves expressing alpha 1, alpha 2, alpha 3, alpha 6 and beta 4 integrins in psoriatic skin compared with control skin. This generalized increase may indicate a secondary trophic effect on all nerves rather than a specific increase in one type of nerve. However, the expression of alpha 2 integrin may be significant in the pathogenesis of the psoriatic plaque, in that it was barely detectable in the normal site-matched biopsies, but much greater in psoriatic plaques. The study of the expression of adhesion molecules by neurones in psoriasis offers a new avenue for investigation of the role of neuronal hypertrophy in the initiation and maintenance of psoriatic plaques.
Trafficking of Cholera Toxin and Related Bacterial Enterotoxins: Pathways and Endpoints (2014)

This chapter focuses on toxins of the cholera family as a paradigm for the opportunistic invasion of the mammalian intestine by exploitation of eukaryotic mechanisms of membrane trafficking, protein transport, and signal transduction. Cholera toxin (CT) enters epithelial cells of the human intestine after binding specific lipids on the host cell apical membrane. The mechanism of toxin entry is a near mirror image of protein biosynthesis in eukaryotic cells. CT represents one of the best-characterized virulence factors produced by pathogenic microorganisms, and its mode of action is discussed in detail. Labile toxins from Escherichia coli that are antigenically similar to CT are classified as type I toxins. Montesano and coauthors first reported the localization of gold-labeled CT in non-clathrin-coated membrane invaginations of cultured liver cells and proposed the existence of at least two distinct pathways for internalization of surface-bound ligands. Human intestinal epithelial cells, however, express low levels of caveolin-1 and -2 and do not exhibit caveolae as assessed morphologically. In eukaryotic cells, translocation of nascent proteins into the endoplasmic reticulum (ER) occurs via the protein conducting channel Sec61, termed as the translocon. Recent studies indicate that protein translocation through the translocon and protein folding in the ER are reversible processes.
Extracting concentrated guided light (1997)

The maximum concentration of radiation is proportional to the square of the refractive index of the medium in which it propagates. A medium with a high refractive index can also serve as a lightguide for concentrated radiation. However, if concentrated radiation is extracted from one medium, with a high refractive index, to another, whose index is lower (e.g., from fused silica into air), part of the radiation may be lost because of the total internal reflection at the interface. We present polygonal shapes suitable for efficient extraction of the concentrated radiation in a controllable way, without increasing the cross-section area (or diameter) of the lightguide. It is shown analytically and experimentally that the use of a secondary concentrator, followed by such a light extractor, both having a high refractive index, can provide considerably more power to a solar receiver with a specific aperture.