gebhart/patrepeval huggingface dataset:
Forskolin inducibility and tissue-specific expression of the fibronectin promoter. (1989)

The mechanism of cyclic AMP (cAMP) induction of fibronectin (FN) in HT-1080 and JEG-3 cells differs (D. C. Dean, R. F. Newby, and S. Bourgeois, J. Cell Biol. 106:2159-2170, 1988). In the fibrosarcoma cell line HT-1080, induction requires both protein synthesis and a lag period of 12 to 24 h. In the choriocarcinoma cell line JEG-3, protein synthesis is not required and induction peaks before 24 h, declining thereafter. We show that the FN promoter is transcribed in vitro and that the transcripts initiate at the proper site. Based on transfection experiments with these cells and FN promoter constructions, a cAMP-responsive element (CRE) was identified between -157 and -188 base pairs upstream of the human FN gene. This sequence also conferred cAMP inducibility in both cell lines on the herpesvirus thymidine kinase promoter when it was placed upstream of a thymidine kinase-chloramphenicol acetyltransferase fusion gene. DNase I protection analysis and gel retardation experiments revealed that the CRE was bound by a protein(s) that was present in both HT-1080 and JEG-3 cells as well as in NIH 3T3 cells. Multiple protein-CRE complexes were resolved by gel retardation with extracts of both cell lines. Forskolin treatment of these cells did not alter qualitatively or quantitatively the pattern of CRE-binding proteins that was observed. The FN promoter was at least 10 times more active in HT-1080 than in JEG-3 cells, even though in JEG-3 cells both the rate of FN biosynthesis and the level of accumulated FN mRNA were greater than those in HT-1080 cells. The difference in promoter activity in HT-1080 and JEG-3 cell was mediated by sequences that were located between positions -510 and -56. Deletion of the FN promoter from positions -510 to -56 resulted in an ~30-fold decrease in promoter activity when this construction was transfected into HT-1080 cells, and similar results were observed in NIH 3T3 cells; however, less than a 2-fold effect was observed in JEG-3 cells. Results of these studies suggest that there is some degree of tissue specificity of FN gene expression and reveal that cAMP induction is mediated, in part, by the same element (CRE) in both HT-1080 and JEG-3 cells.
A low-delay CELP coder for the CCITT 16 kb/s speech coding standard (1992)

A low-delay code-excited linear prediction (LD-CELP) speech coder which is expected to be standardized in 1992 as a CCITT G Series Recommendation for universal applications of speech coding at 16 kb/s is presented. The coder achieves a one-way coding delay of less than 2 ms by making both the LPC predictor and the excitation gain backward-adaptive and by using a small excitation vector size of five samples. The official CCITT laboratory tests revealed that the speech quality of this 16 kb/s LD-CELP coder is either equivalent to or better than that of the CCITT G.721 standard 32-kb/s ADPCM coder for almost all conditions tested. A description of the LD-CELP algorithm, its implementation on the DSP32C for CCITT testing, and performance results from these tests are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A simple backprojection algorithm for 3D in vivo EPID dosimetry of IMRT treatments (2009)

Treatment plans are usually designed, optimized, and evaluated based on the total 3D dose distribution, motivating a total 3D dose verification. The purpose of this study was to develop a 2D transmission-dosimetry method using an electronic portal imaging device (EPID) into a simple 3D method that provides 3D dose information. In the new method, the dose is reconstructed within the patient volume in multiple planes parallel to the EPID for each gantry angle. By summing the 3D dose grids of all beams, the 3D dose distribution for the total treatment fraction is obtained. The algorithm uses patient contours from the planning CT scan but does not include tissue inhomogeneity corrections. The 3D EPID dosimetry method was tested for IMRT fractions of a prostate, a rectum, and a head-and-neck cancer patient. Planned and in vivo-measured dose distributions were within 2% at the dose prescription point. Within the 50% isodose surface of the prescribed dose, at least 97% of points were in agreement, evaluated with a 3D gamma method with criteria of 3% of the prescribed dose and 0.3 cm. Full 3D dose reconstruction on a 0.1 x 0.1 x 0.1 cm3 grid and 3D gamma evaluation took less than 15 min for one fraction on a standard PC. The method allows in vivo determination of 3D dose-volume parameters that are common in clinical practice. The authors conclude that their EPID dosimetry method is an accurate and fast tool for in vivo dose verification of IMRT plans in 3D. Their approach is independent of the treatment planning system and provides a practical safety net for radiotherapy.
Efficiency and selectivity of RNA packaging by Rous sarcoma virus Gag deletion mutants (1994)

In all retrovirus systems studied, the leader region of the RNA contains a cis-acting sequence called psi that is required for packaging the viral RNA genome. Since the pol and env genes are dispensable for formation of RNA-containing particles, the gag gene product must have an RNA binding domain(s) capable of recognizing psi. To gain information about which portion(s) of Gag is required for RNA packaging in the avian sarcoma and leukemia virus system, we utilized a series of gag deletion mutants that retain the ability to assemble virus-like particles. COS cells were cotransfected with these mutant DNAs plus a tester DNA containing psi, and incorporation of RNA into particles were measured by RNase protection. The efficiency of packaging was determined by normalization of the amount of psi+ RNA to the amount of Gag protein released in virus-like particles. Specificity of packaging was determined by comparisons of psi+ and psi- RNA in particles and in cells. The results indicate that much of the MA domain, much of the p10 domain, half of the CA domain, and the entire PR domain of Gag are unnecessary for efficient packaging. In addition, none of these deleted regions is needed for specific selection of the psi RNA. Deletions within the NC domain, as expected, reduce or eliminate both the efficiency and the specificity of packaging. Among mutants that retain the ability to package, a deletion within the CA domain (which includes the major homology region) is the least efficient. We also examined particles of the well-known packaging mutant SE21Q1b. The data suggest that the random RNA packaging behavior of this mutant is not due to a specific defect but rather is the result of the cumulative effect of many point mutations throughout the gag gene.
Robust multi-bit programmable flash memory using a resonant tunnel barrier (2006)

A novel multi-bit flash memory using a SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /a-Si/SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> resonant tunnel barrier was fabricated for the first time. The SONOS-type memory with a resonant tunnel barrier is programmed only at preferential bias conditions determined by quantum tunneling conditions. By doing so, the dispersion of multi-level programmed threshold voltages, Vth, are drastically reduced, and highly reliable data storage is possible. Moreover, program/erase speed, data retention, endurance and read disturb characteristics were also shown to be better than that of a conventional SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> tunnel barrier
Controlled Nanometric Phase Transitions of Phospholipid Membranes by Plasmonic Heating of Single Gold Nanoparticles (2009)

The development of remotely controlled nanoscopic sources of heat is essential for investigating and manipulating temperature sensitive processes at the nanoscale. Here, we use single gold nanoparticles to rapidly deposit controlled amounts of heat in nanoscopic regions of defined size. This allows us to induce and control nanoscale reversible gel-fluid phase transitions in phospholipid membranes. We exploit the optical control over the phase transition to determine the velocity of the fluid phase front into the gel phase membrane and to guide the nanoparticles to specific locations. These results illustrate how single gold nanoparticles enable local thermodynamic investigation and manipulation on nanoscale (bio-) systems.
Imaging diversity receivers for high-speed infrared wireless communication (1998)

We discuss two modifications to the design of wireless infrared links that can yield significant performance improvements, albeit at the price of increased complexity. In line-of-sight and non-line-of-sight links, replacement of a single-element receiver by one employing an imaging light concentrator and a segmented photodetector can reduce received ambient light noise and multipath distortion. For a fixed receiver entrance area, such an imaging receiver can reduce transmit power requirements by as much as about 14 dB, depending on the link design and the number of photodetector segments. Imaging receivers also reduce co-channel interference, and may therefore enable infrared wireless networks to employ space-division multiplexing, wherein several transmitters located in close proximity can transmit simultaneously at the same wavelength. In nondirected non-line-of-sight links, replacement of the diffuse transmitter by one that projects multiple narrow beams can reduce the path loss, further reducing the transmit power requirement by several decibels. We describe the design of an experimental 100 Mb/s infrared wireless link employing a multibeam transmitter and a 37-pixel imaging receiver.
Herbicide Combinations for Postemergent Weed Control in Safflower (Carthamus tinctorius) (1990)

Greenhouse and field studies were conducted to examine the interaction of sethoxydim or fluazifop-P tank mixed with chlorsulfuron or thiameturon for selective weed control in safflower. Under greenhouse conditions, the addition of chlorsulfuron to sethoxydim or fluazifop-P slightly improved the control of green foxtail above that achieved with either herbicide alone. Control of wild oat and wild mustard was similar with the tank mixes and each herbicide alone. In the field, control of wild oat with sethoxydim or fluazifop-P was not altered by adding chlorsulfuron or thiameturon in tank mixes. Similarly, control of common lambsquarters and wild mustard with chlorsulfuron or thiameturon was not affected by adding either sethoxydim or fluazifop-P in tank mixes. Plant height, date of flowering, seed yield, and quality data indicated that safflower tolerated these herbicides applied alone or in combination. Sethoxydim or fluazifop-P tank mixed with chlorsulfuron or thiameturon provide a postemergent alternative for selective control of grass and broadleaf weeds in safflower.
One-Dimensional Metallofullerene Crystal Generated Inside Single-Walled Carbon Nanotubes (2000)

Electron microscope imaging for gadolinium metallofullerenes encapsulating in single-wall carbon nanotubes [(Gd@C82)n@SWNTs] identifies the single Gd atom encaged in each. The intermolecular distance between Gd@C82 is extremely regular, regarding the chains of Gd@C82 as novel one-dimensional crystals. Chemical state analysis of Gd atoms suggests evidence for charge transfer from Gd to either a fullerene cage or a nanotube. The slopes of the temperature dependence of electric resistance for the mat-like films of (Gd@C82)n@SWNTs and (C60)n@SWNTs are much steeper than that for empty SWNTs, suggesting the electron scattering due to the electrostatic potential from inside fullerenes playing an important role.
Tissue factor in cancer angiogenesis and metastasis (1996)

Tumor cells frequently express tissue factor, a transmembrane glycoprotein that functions as the cellular receptor and catalytic cofactor for the serine protease factor VIIa. In human tumors, tissue factor expression correlates spatially with neovascularization, indicating that tissue factor function may be linked to angiogenic properties of malignant tumors. Tissue factor also supports hematogenous tumor dissemination. The role of tissue factor in metastasis appears to involve both the coagulation pathway triggered by interactions of the tissue factor extracellular domain as well as cellular events dependent on the short cytoplasmic domain that may participate in tissue factor-mediated outside-in signaling.
Investigation of Antiulcer and Antioxidant Activity of <i>Juniperus phoenicea</i> L. (1753) Essential Oil in an Experimental Rat Model (2015)

Juniperus phoenicea is a tree of the Cupressaceae family that is popularly known in the south of Tunisia because of its wide application in herbal medicine, including the use of its leaves to treat many diseases such as diarrhea, rheumatism, and intestinal disorders. The aim of this study was to evaluate the ulceroprotective and antioxidant activity of essential oil extracted from the leaves of J. phoenicea (EOJp) against hydrogen chloride (HCl)/ethanol-induced ulcers in rats. The antiulcer activities of 50, 75 and 100 mg/kg body weight (b.w.) EOJp were investigated on 0.3 M HCl/ethanol-induced ulcers in rats. The essential oil yield was 0.69% with 48 compounds; α-pinene was the principal component (20.24%). In vivo pretreatment with EOJp given orally provided dose-dependent protection against HCl/ethanol-induced gastric ulcers in rats. Furthermore, pretreatment with EOJp significantly decreased malondialdehyde (MDA) content and increased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). The activity of the antiulcerogenic EOJp could be from synergistic antioxidant and anti-secretory effects. Oral use of EOJp has excellent preventive effects on induced gastric ulcers comparable to those of the proton pump inhibitor (PPI) omeprazole.
Overview of Appalachian Basin High-Angle and Horizontal Air and Mud Drilling (1991)

ABSTRACT The United States Department of Energy's Morgantown Energy Technology Center has been investigating the potential of using high angle and horizontal drilling technology to improve gas production from low permeability reservoirs for more than 20 years. A chronology of 45 high angle and horizontal wells have been identified to show the date, type well, type build curve, location, formation and the type of application. The historical well drilling events that have taken place since the first well are discussed to evaluate the progress in developing the technology. Detailed discussion about how the drilling technology developed in the Appalachian Basin for directional drilling and completion was provided. A discussion of the types of applications for high and horizontal drilling in the Appalachian Basin were identified. A summary of four jointly funded DOE/Industry horizontal wells were discussed to illustrate how the air horizontal drilling technology developed and learning curves for drilling cost and feet per day were provided to illustrate the improvement in the technology and equipment reliability.
Genistein-Inhibited Cancer Stem Cell-Like Properties and Reduced Chemoresistance of Gastric Cancer (2014)

Genistein, the predominant isoflavone found in soy products, has exerted its anticarcinogenic effect in many different tumor types in vitro and in vivo. Accumulating evidence in recent years has strongly indicated the existence of cancer stem cells in gastric cancer. Here, we showed that low doses of genistein (15 µM), extracted from Millettia nitida Benth var hirsutissima Z Wei, inhibit tumor cell self-renewal in two types of gastric cancer cells by colony formation assay and tumor sphere formation assay. Treatment of gastric cancer cells with genistein reduced its chemoresistance to 5-Fu (fluorouracil) and ciplatin. Further results indicated that the reduced chemoresistance may be associated with the inhibition of ABCG2 expression and ERK 1/2 activity. Furthermore, genistein reduced tumor mass in the xenograft model. Together, genistein inhibited gastric cancer stem cell-like properties and reduced its chemoresistance. Our results provide a further rationale and experimental basis for using the genistein to improve treatment of patients with gastric cancer.
Polymerization of n-Butyl Acrylate by Atom Transfer Radical Polymerization. Remarkable Effect of Ethylene Carbonate and Other Solvents (1998)

Atom transfer radical polymerization (ATRP) of n-butyl acrylate with the homogeneous catalyst, CuIBr/4,4‘-di(5-nonyl)-2,2‘-bipyridine (dNbpy), in benzene, afforded well-defined poly(n-butyl acrylate) with predetermined molecular weights and low polydispersities, Mw/Mn = 1.1. The number-average molecular weight, Mn (measured by GPC and MALDI-TOFMS), was close to the theoretical molecular weight, predetermined by DPn = Δ[M]/[I]0. A similar polymerization in benzene using 2,2‘-bipyridine (bpy) as ligand instead of dNbpy displayed poor control, yielding a polymer whose molecular weight was higher than the theoretical value and demonstrating high polydispersity (Mw/Mn = 2.4). Several solvents were subsequently employed for the polymerizations with the CuIBr/bpy catalyst system. Good control of molecular weight and polydispersity were achieved using ethylene carbonate, and in this system the observed rate of polymerization was even faster than in bulk. The rate of polymerization with the CuIBr/bpy catalyst in ethylene carbonate was first order with respect to the catalyst concentration; a reduction in the concentration of the catalyst led to an increase in the polydispersity of the resulting polymer. Polydispersity decreased on addition of CuIIBr2 or by reducing the amount of ethylene carbonate used. The polymerization in ethylene carbonate with CuPF6/bpy catalyst gave results similar to those observed for the CuIBr/bpy catalyst system. The rapid rate of polymerization in ethylene carbonate was attributed to a monomeric structure of the CuIBr/bpy catalyst, as found for CuIPF6.
Measurement of pharmacokinetics of yttrium-86 radiopharmaceuticals with PET and radiation dose calculation of analogous yttrium-90 radiotherapeutics. (1993)

This study was performed to demonstrate the quantitative in vivo assessment of human pharmacokinetics of 90Y-radiotherapeutics using the positron-emitting substitute 86Y and PET. This technique is illustrated in a patient with disseminated bone metastases from breast cancer who was injected with 100 MBq of 86Y-citrate as an analog of the commercially available radiotherapeutic 90Y-citrate. Whole-body distribution was measured with a PET camera 4, 10, 21, 28 and 45 hr postinjection. Uptake data were determined from reconstructed transverse PET images by regions of interest placed in normal bone tissue, liver and metastases. Images of coronal and sagittal whole-body sections were obtained by reformatting the transverse PET images. The ratio of activity concentration in metastases to that in normal bone ranged from 1.5:1 to 3.5:1. Of the injected tracer, 13.4% was found in the skeleton and 0.43% in the metastasis with the highest 86Y concentration. Radiation doses per 1 MBq of injected 90Y-citrate were calculated from 86Y-citrate data and data from MIRD pamphlets 5 and 11. The doses were 1.01 MGy/MBq for red marrow, 593 microGy/MBq for the liver and approximately 3.5 MGy/MBq for the most conspicuous metastases. This study demonstrates that the use of PET via 86Y allows an individual in vivo quantification of activity uptake and radiation dose of both normal tissue and tumor in pain treatment with 90Y-labeled radiotherapeutics.
Nitric oxide synthase gene transfer to the vessel wall (1999)

A wide array of vascular disorders have been shown to benefit from gene therapy. Given the vasoprotective role that nitric oxide plays in the vasculature, it is understandable why gene therapy with the three different isoforms of nitric oxide synthase has been so successful. This review summarizes the current literature pertaining to nitric oxide synthase gene transfer to the vascular wall.
Progress toward a Malaria Vaccine: Efficient Induction of Protective Anti-Malaria Immunity (2001)

Malaria can be a very severe disease, particularly in young children, pregnant women (mostly in primipara), and malaria naïve adults, and currently ranks among the most prevalent infections in tropical and subtropical areas throughout the world. The widespread occurrence and the increased incidence of malaria in many countries, caused by drug-resistant parasites (Plasmodium falciparum and P. vivax) and insecticide-resistant vectors (Anopheles mosquitoes), indicate the need to develop new methods of controlling this disease. Experimental vaccination with irradiated sporozoites can protect animals and humans against the disease, demonstrating the feasibility of developing an effective malaria vaccine. However, developing a universally effective, long lasting vaccine against this parasitic disease has been a difficult task, due to several problems. One difficulty stems from the complexity of the parasite's life cycle. During their life cycle, malaria parasites change their residence within the host, thus avoiding being re-exposed to the same immunological environment. These parasites also possess some distinct antigens, present at different life stages of the parasite, the so-called stage-specific antigens. While some of the stage-specific antigens can induce protective immune responses in the host, these responses are usually genetically restricted, this being another reason for delaying the development of a universally effective vaccine. The stage-specific antigens must be used as immunogens and introduced into the host by using a delivery system that should efficiently induce protective responses against the respective stages. Here we review several research approaches aimed at inducing protective anti-malaria immunity, overcoming the difficulties described above.
Enhanced malignant transformation induced by expression of a distinct protein domain of ribonucleotide reductase large subunit from herpes simplex virus type 2. (1991)

The 1.3-kilobase (kb) Pst I DNA fragment C (Pst I-C) of herpes simplex virus type 2 (HSV-2) morphological transforming region III (mtrIII; map unit 0.562-0.570) encodes part of the N-terminal half of the large subunit of ribonucleotide reductase (RR1; amino acid residues 71-502) and induces the neoplastic transformation of immortalized cell lines. To assess directly the role of these RR1 protein sequences in cell transformation, the Pst I-C fragment was cloned in an expression vector (p91023) containing an adenovirus-simian virus 40 promoter-enhancer to generate recombinant plasmid p9-C. Expression of a protein domain (approximately 65 kDa) was observed in p9-C-transfected COS-7 and Rat2 cells but not in those transfected with plasmid pHC-14 (Pst I-C in a promoterless vector). In Rat2 cells, p9-C induced highly transformed foci at an elevated frequency compared with that of pHC-14. Introduction of translation termination (TAG) condons within the RR1 coding sequence and within all three reading frames inactivated RR1 protein expression from p9-C and reduced its transforming activity to the level seen with the standard pHC-14 construct. Wild-type p9-C specified a protein kinase capable of autophosphorylation. Computer-assisted analysis further revealed significant similarity between regions of mtrIII-specific RR1 and amino acid patterns conserved within the proinsulin precursor family and DNA transposition proteins. These results identify a distinct domain of the HSV-2 RR1 protein involved in the induction of enhanced malignant transformation. In addition, the data indicate that the mtrIII DNA itself can induce basal-level transformation in the absence of protein expression.