gebhart/patrepeval huggingface dataset:
Antisense peptide recognition of sense peptides: sequence simplification and evaluation of forces underlying the interaction (1989)

Structural principles were studied which underlie the recognition of sense peptides (sense DNA encoded) by synthetic peptides encoded in the corresponding antisense strand of DNA. The direct-readout antisense peptides corresponding to ribonuclease S-peptide bind to an affinity matrix containing immobilized S-peptide with significant selectivity and with dissociation constants in the range of 10(-6) M as judged by analytical affinity chromatography. Synthetic, sequence-modified forms of antisense peptides also exhibit substantial binding affinity, including a "scrambled" peptide in which the order of residue positions is changed while the overall residue composition is retained. The antisense mutants, as the original antisense peptides, bind at saturation with greater than 1:1 stoichiometry to immobilized S-peptide. The data suggest significant sequence degeneracy in the interaction of antisense with sense peptide. In contrast, selectivity was confirmed by the inability of several control peptides to bind to immobilized S-peptide. The idea was tested that the hydropathic pattern of the amino acid sequence serves to induce antisense peptide recognition. A hydropathically sequence-simplified mutant of antisense peptide was made in which all strongly hydrophilic (charged) residues were replaced by Lys, all strongly hydrophobic residues by Leu, and all weakly hydrophilic and hydrophobic residues by Ala, except Gly which was unchanged. This "KLAG" mutant also binds to immobilized S-peptide, with an affinity only an order of magnitude less than that with the original antisense peptide and with multiple stoichiometry. Mutants of the KLAG model, in which the hydropathic pattern was changed substantially, exhibited a lower binding affinity for S-peptide.(ABSTRACT TRUNCATED AT 250 WORDS)
Transcutaneous electrical stimulation and acupuncture: Comparison of treatment for low-back pain (1976)

Twelve patients suffering chronic low-back pain were treated with both acupuncture and transcutaneous electrical stimulation. The order of treatments was balanced, and changes in the intensity and quality of pain were measured with the McGill Pain Questionnaire. The results, based on a measure of overall pain intensity, show that pain relief greater than 33% was produced in 75% of the patients by acupuncture and in 66% by electrical stimulation. The mean duration of pain relief was 40 h after acupuncture and 23 h after electrical stimulation. Although the mean scores are larger for acupuncture than for transcutaneous stimulation, statistical analyses of the data failed to reveal significant differences between the two treatments on any of the measures. Both methods, therefore, appear to be equally effective, and probably have the same underlying mechanism of action. Consideration of the advantages and disadvantages of the two methods suggests that that transcutaneous electrical stimulation is potentially the more practical, since it can be administered under supervision by paramedical personnel.
Nucleotide sequences of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1a and 1b (1992)

The nucleotide sequence of a 3,378-bp DNA fragment of Streptococcus pneumoniae that included the structural gene for penicillin-binding protein (PBP) 1a (ponA), which encodes 719 amino acids, was determined. Homologous DNA fragments from an S. oralis strain were amplified with ponA-specific oligonucleotides. The 2,524-bp S. oralis sequence contained the coding region for the first 636 amino acids of a PBP. The coding sequence differed by 437 nucleotides (27%) and one additional triplet, resulting in 87 amino acid substitutions (14%), from S. pneumoniae PBP 1a. Both PBPs are highly homologous to bifunctional high-M(r) Escherichia coli PBPs 1a and 1b.
Resistance of Tumor Interstitial Pressure to the Penetration of Intraperitoneally Delivered Antibodies into Metastatic Ovarian Tumors (2005)

The results only partially support our hypothesis and imply that the microenvironment of the tumor is in itself a major barrier to delivery of charged macromolecules.
Strange bedfellows: issues in object naming under Unix (2002)

Naming plays a key role in the design of any system that exports services or resources. Object systems may export many different categories of names: instances, components of records, types, etc. Operating systems export the names of files, devices, and services. Integrating an object base with existing operating system facilities can improve accessibility of the object base resources. We consider the benefits and pitfalls of integrating an object base namespace with the Unix namespace.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
MPEG: a technological basis for multimedia applications (1995)

The Moving Picture Experts Group was established in 1988 in the framework of the Joint ISO/IEC Technical Committee (JTC 1) on Information Technology. They were given the mandate to develop standards for coded representation of moving pictures, associated audio, and their combination when used for storage and retrieval on digital storage media (DSM). In April 1990 they became Working Group 11 of JTC 1/SC 2, then in November 1991, WG 11 of JTC 1/SC 29. The three original work items of the group-coding up to 1.5, 10, and 40 Mbps-were nicknamed MPEG-1, -2 and -3, respectively. The group dropped the MPEG3 work item in July 1992 when it became apparent that the functionalities supported by the MPEG-2 requirements made it redundant. The current MPEG-4 work item, first proposed in May 1991 and approved in July 1993, targets audiovisual coding at very low bit-rates.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Effect of “Free” Cation Substituent on Gas Separation Performance of Polymer−Room-Temperature Ionic Liquid Composite Membranes (2009)

Room-temperature ionic liquid (RTIL) based monomers were photopolymerized in the presence of nonpolymerizable RTILs with various types of organic functional groups attached to the imidazolium cation. Groups employed include alkyl, ether, nitrile, fluoroalkyl, and siloxane functionalities. This straightforward method allows for a broad range of functional groups to be incorporated into poly(RTIL) matrices without the need to develop new monomers. The resultant poly(RTIL)−RTIL composites were homogeneous, optically transparent solids that exhibited no signs of phase separation, even after many months of storage. As thin films, poly(RTIL)−RTIL composites were utilized as gas separation membranes and tested for their permeabilities to CO2, N2, and CH4. The presence of 20 mol % "free" RTIL within the poly(RTIL) matrix was shown to increase CO2 permeability by ∼100−250% relative to the neat poly(RTIL) membrane with no free RTIL component. The nature of the organic functional group associated with the free RTIL cation can influence both gas permeability and CO2/N2 and CO2/CH4 selectivity.
Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus (1987)

Human cytomegalovirus particles contain a phosphoprotein of 150,000 (pp150) apparent molecular weight in their matrix; the protein appears particularly reactive in Western blot analyses with human antisera. The gene for pp150 was mapped by screening a bacteriophage lambda gt11 cDNA expression library with monospecific rabbit antisera. Subsequent hybridization of cDNA with cosmid and plasmid clones containing the human cytomegalovirus strain AD169 genome mapped the gene to HindIII fragments J and N. The gene is transcribed into a late 6.2-kilobase RNA. The nucleotide sequence of this region was determined, and a transcription initiation site and two polyadenylation sites of an abundant transcript were located by primer extension and nuclease protection experiments. The reading frame for pp150, deduced from computer analyses, gives rise to a polypeptide of 1,048 amino acids in length; protein secondary structure analysis revealed multiple beta-pleated sheets in hydrophilic clusters, providing a possible explanation for the immunogenic properties of the polypeptide.
Photodamage Induced by Zinc(II)-phthalocyanine to Microtubules, Actin, α-Actinin and Keratin of HeLa Cells¶ (2001)

We have studied the photosensitizing effects of zinc(II)-phthalocyanine (ZnPc) on the cytoskeleton of HeLa cells using sublethal (10(-7) M, followed by 1 or 3 min of red light to induce 20%, LD20, or 60%, LD60, cell death, respectively) or lethal (5 x 10(-6) M and 15 min of irradiation, LD100) experimental conditions. The immunofluorescent analysis of the cytoskeleton showed a variable photodamage to microtubules (MT), actin microfilaments (AF) and intermediate filaments of keratin (KF), as well as on alpha-actinin, which was dependent on treatment conditions. Both sublethal treatments induced deep alterations on interphase and mitotic MT. The mitotic index increased with time with the maximum at 18 h (12%) or 24 h (14%) after LD20 or LD60, respectively. The alterations on AF and alpha-actinin were much more severe than those observed on KF at any evaluated time. With the exception of the KF, which remained partially organized, the MT and AF network was severely damaged by the lethal treatment. Western blot analysis for alpha-tubulin, G-actin and alpha-actinin from soluble and insoluble fractions confirmed the results observed by immunofluorescence, thus indicating that these cytoskeletal components are involved in cell damage and death by ZnPc photosensitization.
4G/Multiband handheld device ground antennas (2013)

Promising internal handheld device ground antennas are presented. Using the ground antenna concept, the antenna element functions mainly as an exciter and can have a very small size to achieve 4G/multiband operation. In this paper, ground antenna structures for handheld devices including the use of a loop strip, a half-loop strip, a simple shorted strip, or an inverted-F strip with the aid of a capacitive element to achieve decreased resonant length and the use of a small-size strip or patch with an inductive element to combine with the device ground plane to form as an asymmetric dipole structure are discussed. By aided with proper matching circuits, the ground antennas are promising to provide wideband operations. A small corner strip antenna with its resonant length 29 mm only (less than 0.08λ at 750 MHz) is also demonstrated for the LTE/WWAN tablet computer application. The ground antennas are also very promising to form high-isolation antenna systems for MIMO and diversity operation in the slim handheld devices.
Breast Cancer Molecular Subtypes Respond Differently to Preoperative Chemotherapy (2005)

The basal-like and erbB2+ subtypes of breast cancer are more sensitive to paclitaxel- and doxorubicin-containing preoperative chemotherapy than the luminal and normal-like cancers.
Regiospecificity and isotope effects associated with the methyl-methylene eliminations in the enzyme-catalyzed biosynthesis of (R)- and (S)-limonene (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRegiospecificity and isotope effects associated with the methyl-methylene eliminations in the enzyme-catalyzed biosynthesis of (R)- and (S)-limoneneHyung Jung Pyun, Robert M. Coates, Kurt C. Wagschal, Paul McGeady, and Rodney B. CroteauCite this: J. Org. Chem. 1993, 58, 15, 3998–4009Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://doi.org/10.1021/jo00067a037RIGHTS & PERMISSIONSArticle Views548Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Methemoglobinemia Related to Local Anesthetics: A Summary of 242 Episodes (2009)

Benzocaine should no longer be used. Prilocaine should not be used in children younger than 6-mo-old, in pregnant women, or in patients taking other oxidizing drugs. The dose should be limited to 2.5 mg/kg.
Semantic contours from inverse detectors (2011)

We study the challenging problem of localizing and classifying category-specific object contours in real world images. For this purpose, we present a simple yet effective method for combining generic object detectors with bottom-up contours to identify object contours. We also provide a principled way of combining information from different part detectors and across categories. In order to study the problem and evaluate quantitatively our approach, we present a dataset of semantic exterior boundaries on more than 20, 000 object instances belonging to 20 categories, using the images from the VOC2011 PASCAL challenge [7].
A monolithically integrated 1 x 4 switchable photodiode array with preamplifier for programmable frequency filters and optical interconnects (1997)

An InGaAs-InP 1×4 p-i-n photodiode array has been monolithically integrated with switching junction field-effect transistors and a transimpedance preamplifier. The switching on-off ratio at the output of the preamplifier is 60 dB. As an optical receiver, the circuit has a 3-dB bandwidth of 1.1 GHz and a sensitivity of -25 dBm at 1 Gb/s, BER=10/sup -9/, and 1.55-μm wavelength. A programmable frequency filter has been demonstrated as an application of this novel circuit.
The Synthesis of N-(2-Benzyl-4-Δ2-oxazolinoyl)-valine1 (1954)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Synthesis of N-(2-Benzyl-4-Δ2-oxazolinoyl)-valine1Homer Adkins, Robert M. Ross, Dorothy Chynoweth Schroeder, C. Lynn Mahoney, and Walter W. GilbertCite this: J. Am. Chem. Soc. 1954, 76, 1, 147–152Publication Date (Print):January 1, 1954Publication History Published online1 May 2002Published inissue 1 January 1954https://pubs.acs.org/doi/10.1021/ja01630a040https://doi.org/10.1021/ja01630a040research-articleACS PublicationsRequest reuse permissionsArticle Views171Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Virosome‐Mimotope Approach to Synthetic Vaccine Design and Optimization: Synthesis, Conformation, and Immune Recognition of a Potential Malaria‐Vaccine Candidate (2003)

An optimized cyclic peptidomimetic (see picture) of the NPNA-repeat region in the circumsporozoite (CS) protein of P. falciparum was designed. When delivered to the immune system on human-compatible viruslike particles (virosomes), the mimetic effectively elicits sporozoite-binding antibodies. This virosome-mimotope (virotope) approach may be of general value for the design and testing of new synthetic-vaccine candidates. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2003/z50348_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The LIM-homeobox gene Lhx8 is required for the development of many cholinergic neurons in the mouse forebrain (2003)

Forebrain cholinergic neurons play important roles as striatal local circuit neurons and basal telencephalic projection neurons. The genetic mechanisms that control development of these neurons suggest that most of them are derived from the basal telencephalon where Lhx8, a LIM-homeobox gene, is expressed. Here we report that mice with a null mutation of Lhx8 are deficient in the development of forebrain cholinergic neurons. Lhx8 mutants lack the nucleus basalis, a major source of the cholinergic input to the cerebral cortex. In addition, the number of cholinergic neurons is reduced in several other areas of the subcortical forebrain in Lhx8 mutants, including the caudate-putamen, medial septal nucleus, nucleus of the diagonal band, and magnocellular preoptic nucleus. Although cholinergic neurons are not formed, initial steps in their specification appear to be preserved, as indicated by a presence of cells expressing a truncated Lhx8 mRNA and mRNA of the homeobox gene Gbx1. These results provide genetic evidence supporting an important role for Lhx8 in development of cholinergic neurons in the forebrain.
Evaluation of 5-(11)C-methyl-A-85380 as an imaging agent for PET investigations of brain nicotinic acetylcholine receptors. (2004)

These results suggest that (11)C-5MA is a potential PET radiopharmaceutical for nuclear medical studies of nAChRs in the brain.
Replicative function and neutralization sensitivity of envelope glycoproteins from primary and T-cell line-passaged human immunodeficiency virus type 1 isolates (1995)

The structure, replicative properties, and sensitivity to neutralization by soluble CD4 and monoclonal antibodies were examined for molecularly cloned envelope glycoproteins derived from human immunodeficiency virus type 1 (HIV-1) viruses either isolated directly from patients or passaged in T-cell lines. Complementation of virus entry into peripheral blood mononuclear cell targets by primary patient envelope glycoproteins exhibited efficiencies ranging from that observed for the HXBc2 envelope glycoproteins, which are derived from a T-cell line-passaged virus, to approximately fivefold-lower values. The ability of the envelope glycoproteins to complement virus entry roughly correlated with sensitivity to neutralization by soluble CD4. Laboratory-adapted viruses were sensitive to neutralization by monoclonal antibodies directed against the CD4-binding site and the third variable (V3) loop of the gp120 glycoprotein. By comparison, viruses with envelope glycoproteins from primary patient isolates exhibited decreased sensitivity to neutralization by these monoclonal antibodies; for these viruses, neutralization sensitivity correlated with replicative ability. Subinhibitory concentrations of soluble CD4 and a CD4-binding site-directed antibody significantly enhanced the entry of viruses containing envelope glycoproteins from some primary patient isolates. The sensitivity of viruses containing the different envelope glycoproteins to neutralization by soluble CD4 or monoclonal antibodies could be predicted by assays dependent on the binding of the inhibitory molecule to the oligomeric envelope glycoprotein complex but less well by assays measuring binding to the monomeric gp120 glycoprotein. These results indicate that the intrinsic structure of the oligomeric envelope glycoprotein complex of primary HIV-1 isolates, while often less than optimal with respect to the mediation of early events in virus replication, allows a relative degree of resistance to neutralizing antibodies. The interplay of selective forces for higher virus replication efficiency and resistance to neutralizing antibodies could explain the temporal course described for the in vivo emergence of HIV-1 isolates with differing phenotypes.
Synthesis, Characterization, and Computational Studies of Cycloparaphenylene Dimers (2012)

Two novel arene-bridged cycloparaphenylene dimers (1 and 2) were prepared using a functionalized precursor, bromo-substituted macrocycle 7. The preferred conformations of these dimeric structures were evaluated computationally in the solid state, as well as in the gas and solution phases. In the solid state, the trans configuration of 1 is preferred by 34 kcal/mol due to the denser crystal packing structure that is achieved. In contrast, in the gas phase and in solution, the cis conformation is favored by 7 kcal/mol (dimer 1) and 10 kcal/mol (dimer 2), with a cis to trans activation barrier of 20 kcal/mol. The stabilization seen in the cis conformations is attributed to the increased van der Waals interactions between the two cycloparaphenylene rings. These calculations indicate that the cis conformation is accessible in solution, which is promising for future efforts toward the synthesis of short carbon nanotubes (CNTs) via cycloparaphenylene monomers. In addition, the optoelectronic properties of these dimeric cycloparaphenylenes were characterized both experimentally and computationally for the first time.
Outer membrane protein D15 is conserved among Haemophilus influenzae species and may represent a universal protective antigen against invasive disease (1997)

We have cloned and sequenced the d15 gene from two strains of Haemophilus influenzae type b (Hib) and two strains of nontypeable H. influenzae (NTHI). The nucleotide and deduced protein sequences of d15 are highly conserved, with only a small variable region identified near the carboxyl terminus of the protein. Analysis of upstream sequences revealed that the H. influenzae d15 gene may be part of a large potential operon of closely spaced open reading frames, including one with significant homology to the Escherichia coli cds gene encoding CDP-diglyceride synthetase. Southern blot analysis demonstrated that the d15 gene is also present in H. influenzae types a, c, d, e, and f and in Haemophilus parainfluenzae. A recombinant D15 (rD15) protein was expressed in good quantity in E. coli from the inducible T7 promoter, and monospecific anti-rD15 antibodies were raised. Immunoblot analysis of H. influenzae serotypes a, b, c, d, e, and f, NTHI, and H. parainfluenzae lysates revealed that they all expressed a cross-reactive D15-like protein. Purified rD15 was found to be highly immunogenic in mice, guinea pigs, and rabbits, and passive transfer of anti-rD15 antibodies protected infant rats from challenge with H. influenzae type b or type a in infant rat models of bacteremia. Thus, D15 is a highly conserved antigen that is protective in animal models and it may be a useful component of a universal subunit vaccine against Haemophilus infection and disease.
Mutation site and context dependent effects of ESR1 mutation in genome-edited breast cancer cell models (2017)

Taken together, ESR1 mutations in genome-edited breast cancer cell lines confer ligand-independent growth and endocrine resistance. These biologically relevant models can be used for further mechanistic and translational studies, including context-specific and mutation site-specific analysis of the ESR1 mutations.