gebhart/patrepeval huggingface dataset:
Molecular analyses of 17p11.2 deletions in 62 Smith-Magenis syndrome patients. (1996)

Smith-Magenis syndrome (SMS) is a clinically recognizable, multiple congenital anomalies/mental retardation syndrome caused by an interstitial deletion involving band p11.2 of chromosome 17. Toward the molecular definition of the interval defining this microdeletion syndrome, 62 unrelated SMS patients in conjunction with 70 available unaffected parents were molecularly analyzed with respect to the presence or absence of 14 loci in the proximal region of the short arm of chromosome 17. A multifaceted approach was used to determine deletion status at the various loci that combined (i) FISH analysis, (ii)PCR and Southern analysis of somatic cell hybrids retaining the deleted chromosome 17 from selected patients, and (iii) genotype determination of patients for whom a parent(s) was available at four microsatellite marker loci and at four loci with associated RFLPs. The relative order of two novel anonymous markers and a new microsatellite marker was determined in 17p11.2. The results confirmed that the proximal deletion breakpoint in the majority of SMS patients is located between markers D17S58 (EW301) and D17S446 (FG1) within the 17p11.1-17p11.2 region. The common distal breakpoint was mapped between markers cCI17-638, which lies distal to D17S71, and cCI17-498, which lies proximal to the Charcot Marie-Tooth disease type 1A locus. The locus D17S258 was found to be deleted in all 62 patients, and probes from this region can be used for diagnosis of the SMS deletion by FISH. Ten patients demonstrated molecularly distinct deletions; of these, two patients had smaller deletions and will enable the definition of the critical interval for SMS.
Uplink and Downlink Coverage Improvements of 802.11g Signals Using a Distributed Antenna Network (2007)

A distributed antenna network (DAN) is demonstrated to improve the coverage of in-building wireless services. A doubling in the number of locations with a high throughput is achieved. A detailed analysis of the performance improvement of a three-antenna DAN over a single-antenna system shows that 10-dB more power would be required from the single antenna to achieve a comparable performance. The effect of the additional delay spread generated by the DAN is also discussed, and the conditions under which it does not degrade performance are investigated.
The Utilization of Abomasal Supplements of Proteins and Amino Acids by Sheep With Special Reference to Wool. Growth (1972)

Proteins of different amino acid composition (Promine-D, wheat gluten, and zein) were given as abomasal infusions to sheep and effects on wool growth rate, body weight gain, and nitrogen retention were compared with those of casein. These results were considered together with earlier data obtained for whole egg protein, egg albumen, maize gluten, and gelatin. The nutritive value of bloodmeal supplements was also studied. In addition the effects on wool growth of adding lysine and tryptophan to zein, and of adding leucine to casein, were examined.
Harmonic and noise coding of LPC residuals with classified vector quantization (2002)

An efficient coding scheme for linear predictive coding (LPC) residuals is proposed based on harmonic and noise representation. New features of the scheme include classified vector quantization of the spectral envelope of LPC residuals with a weighted distortion measure. The improvement in performance obtained by classifying codebooks based on a voiced/unvoiced (V/UV) decision is shown. Sequences of the short-term RMS power of the time domain waveforms are also vector quantized and transmitted for unvoiced signals. A fast synthesis algorithm for voiced signals using an FFT is also presented, which reduces the high complexity of the direct sinusoidal synthesis method with interpolated magnitudes and phases. Informal listening tests indicate that, in combination with a known LSP quantization technique, this residual coding scheme provides good communication quality at a total bit rate of less than 2.0 kbps.
<title>Fundamental system for biosensor characterization: application to surface plasmon resonance (SPR)</title> (1996)

The aim of the described research is to develop a general system for characterizing and developing signal transduction systems for microbiosensors. The approach that we are using is applicable to signal transduction systems based on surface plasmon resonance, chemiluminescence, fluorescence, mass as well as other phenomena. The specific goal of our approach is to develop a general system that will allow for the systematic characterization of the effects of the affinity of the sensor specificity element for the target analyte, the effect of analyte mass on signal size and the general performance of the sensor system with respect to sensitivity and selectivity. At the same, time this system should allow for the characterization of the distribution of biospecificity elements on the sensor surface. We chose the anti-fluorescein monoclonal antibody approach for this development system, since the antigen fluorescein can be attached to many different molecules and organisms through free amine groups via reaction with fluorescein isothiocyanate. Also, well characterized monoclonal antibodies with a broad range of Kd values are available. We also describe rapid procedures for generating proteins for use in biosensor applications.
Scaling Up the Ligase Chain Reaction-Based Approach to Gene Synthesis (2001)

liter each of these suspensions was used as a template for PCR. Sequencing of the plasmid DNA can now be performed using two PCR primers flanking the MCS of the vector (we used M13 universal forward primer and M13 universal reverse primer; Figure
Phase Behavior of Polymers in Supercritical Fluid Solvents (1999)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhase Behavior of Polymers in Supercritical Fluid SolventsChristopher F. Kirby and Mark A. McHughView Author Information Department of Chemical Engineering, 3400 N. Charles Street, Johns Hopkins University, Baltimore, Maryland 21218 Cite this: Chem. Rev. 1999, 99, 2, 565–602Publication Date (Web):January 13, 1999Publication History Received6 July 1998Revised13 October 1998Published online13 January 1999Published inissue 1 February 1999https://pubs.acs.org/doi/10.1021/cr970046jhttps://doi.org/10.1021/cr970046jresearch-articleACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views2643Altmetric-Citations336LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Copolymers,Hydrocarbons,Organic compounds,Polymers,Solvents Get e-Alerts
Sensor Technology in Assessments of Clinical Skill (2015)

To the Editor: Blair et al. (Dec. 4 issue) 1 are thorough and insightful in their review of conduct disorder and related entities, except that there is no reference to sex, sex ratios, or male sex. Yet for childhood-onset conduct disorder that persists throughout life, there is consensus that there is a massive male predominance, by a factor of 10 to 15, whereas for conduct disorder generally, the ratio is still between 2:1 and 4:1. 2 Mounting evidence suggests that prenatal androgenization affects the brains of human male fetuses as it does the brains of many other mammals, resulting in a partially gendered brain. 3 The hypothalamus and amygdala, which Blair et al. identify as participating in hypothesized conduct disorder-related neural circuits, are affected by this organizational effect of androgens, and activational effects of pubertal and postpubertal androgens further influence their function. Violence, which is overwhelmingly used by young men, cannot be understood in terms of brain function, prevention, or treatment without reference to and investigation of sex differences and male-predominant neuroendocrine effects.
Steroid Hormone Action (1993)

Abstract A comprehensive overview of the molecular mechanisms by which these hormones regulate the expression of specific target genes. The book covers the structure of steroid receptors, transcriptional activation and repression, and also contains chapters on steroid binding proteins and steroidogenesis. Steroid receptors represent one of the best characterized transcription factors so that the book will be of general interest to molecular biologists studying eucaryotic gene regulation. Chapters on the mechanisms of action of steroid antagonists and defective receptors in target cells will be of particular interest to endocrinologists and clinicians. In such a rapidly progressing field it is difficult to keep up with all the literature but this book, written by international experts, focuses on the most important advances that have been made recently.
Platelet Production (1970)

PhysiologyMEGAKARYOCYTES arise from mononuclear precursors and undergo nuclear replication within a common cytoplasm as the initial identifiable phase of the life cycle of the cells. The number of nuclei doubles with each mitosis. In normal bone marrow about 65 per cent of the megakaryocytes contain eight diploid nuclei, 25 per cent have 16 nuclei, and 10 per cent have four nuclei.1 , 2 The cell then accumulates cytoplasm in direct proportion to the number of nuclei formed during nuclear proliferation — that is, the cell consists of four, eight or 16 "nuclear units."3 Cell maturation, which occurs mainly after nuclear proliferation, . . .
Vizster: Visualizing Online Social Networks (2006)

Recent years have witnessed the dramatic popularity of online social networking services, in which millions of members publicly articulate mutual friendship relations. Guided by ethnographic research of these online communities, we have designed and implemented a visualization system for playful end-user exploration and navigation of large scale online social networks. Our design builds upon familiar node link network layouts to contribute customized techniques for exploring connectivity in large graph structures, supporting visual search and analysis, and automatically identifying and visualizing community structures. Both public installation and controlled studies of the system provide evidence of the system's usability, capacity for facilitating discovery, and potential for fun and engaged social activity
Automated High-Throughput Infusion ESI-MS with Direct Coupling to a Microtiter Plate (2001)

This paper describes the design and application of instrumentation for automated high-throughput infusion ESI-mass spectrometry. The approach, based on a subatmospheric ESI interface, allows sample introduction from a commercially available microtiter plate without the need for a separate fluid delivery system. The microtiter plate was placed vertically on a three-dimensional translation stage in front of the sampling ESI interface. A single, 7-cm, 20-microm-i.d. fused-silica capillary (total volume, 70 nL), with a tapered tip, served as a combination of sample delivery and spraying capillary. The tapered tip of the capillary was enclosed in a subatmospheric chamber attached in front of the orifice of the mass spectrometer. The sample aspiration rate (flow rate) was regulated by computer-controlled pneumatic valves, which allowed fast switching of the pressure in the subatmospheric ESI chamber. A flow-through wash device was positioned between the microtiter plate and the ESI interface. This design allowed alternate filling of the capillary with (a) sample from the wells and (b) wash solution from the wash device. Sample turnaround times of 10 s/sample, with a 120-nL sample consumption/analysis, and a duty cycle (percentage of total analysis time spent acquiring data) of 40% were achieved. The infusion system was demonstrated in the analysis of preparative HPLC fractions from a small molecule combinatorial library.
A wide field electron microscopic analysis of the fiber constituents of the major splanchnic nerve in cat (1982)

The fiber composition of the left major splanchnic nerve was studied in cats by electron microscopy. Comparisons were made between normal and partially degenerated nerve specimens following ventral rhizotomy (T3-L1), or spinal nerve division (T3-L1). Normal, major splanchnic nerves contained 2,500-4,000 myelinated and 10,000-15,000 unmyelinated fibers. Preganglionic fibers included approximately 90% of the finely myelinated (1-7 micrometers) and over 50% of the unmyelinated fibers. Removal of the sensory and preganglionic components by spinal nerve division revealed a third postganglionic fiber category. This included 13-38 small myelinated (1-5 micrometers) and 1,645-7,619 unmyelinated fibers. Finally, a comparison of normal and partially degenerated nerve specimens of both groups (ventral rhizotomy and spinal nerve cut) indicated that splanchnic afferents are made up of virtually all of the 120-350 large myelinated (8-14 micrometers) and 10% of the small myelinated (1-7 micrometers) fibers. A preliminary estimate indicated that about 10-20% of the unmyelinated fibers were sensory. The implications of these findings are discussed.
Two-Dimensional Polymerization of Lipid Bilayers:Degree of Polymerization of Sorbyl Lipids (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTwo-Dimensional Polymerization of Lipid Bilayers:Degree of Polymerization of Sorbyl LipidsHenry Lamparski and David F. O'BrienCite this: Macromolecules 1995, 28, 6, 1786–1794Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/ma00110a010https://doi.org/10.1021/ma00110a010research-articleACS PublicationsRequest reuse permissionsArticle Views277Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Detection of serum TNF-α, IFN-γ, IL-6 and IL-8 in patients with hepatitis B (1999)

AIM:To assess the possible roles of cytokines (TNF-alpha, IFN-beta, IL-6 and IL-8) in liver damage of hepatitis B.METHODS:The serum TNF-alpha, IFN-beta, IL-6 and IL-8 were detected by ELISA in 66 patients with hepatitis B and 20 healthy blood donors.RESULTS:TNF-alpha and IL-6 in all types of clinical hepatitis B were significantly higher than those in healthy blood donors (P < 0.05); meanwhile the levels of TNF-alpha, IFN-beta, IL-6 and IL-8 in the patients with fulminant hepatitis B were much higher than those in the patients with acute hepatitis B (P < 0.05); the level of TNF-alpha was positively correlated with the levels of IFN-beta, Il-6 and IL-8 in all types of hepatitis B (r(IFN) = 0.24,r(IL6) = 0.35,r(IL8) = 0.44) and the TNF-alpha, IFN-beta, IL-6 and IL-8 were positively correlated with serum bilirubin (P < 0.05). Dynamic changes of these cytokines were observed in the course of acute and fulminant hepatitis. The level of IFN-beta peaked in the initial period of acute hepatitis and early stage of hepatic coma in fulminant hepatitis; TNF-alpha, IL-6 and IL-8 increased with exacerbation, and reached a peak when the liver damage was most serious, then decreased when patient conditions were improved.CONCLUSION:The increased cytokines were related to the inflammation of liver cells and multiple factors may play certain roles in liver damage.
Integrated optic laser emitting at 906, 1057, and 1358 nm (1993)

Laser oscillation at 906, 1057, and 1358 nm has been achieved in the same neodymium-doped-glass integrated optic laser. This is believed to be the first report of a 906 nm integrated optic laser. High slope efficiency and high output power were observed at 1057 nm. The laser was fabricated by silver ion exchange in a phosphate glass.
Expression of the regulatory protein LndI for landomycin E production inStreptomyces globisporus1912 is controlled by the availability of tRNA for the rare UUA codon (2006)

The gene lndI encodes the activator of landomycin biosynthesis. The utilization of LndI-EGFP fusions led us to investigate the temporal pattern of this gene expression and demonstrated the delay between lndI transcription and translation. The TTA codon in lndI is thought to be the reason for this delay. The replacement of TTA with CTC cancelled the pause between lndI transcription and the translation. The wild-type of the lndI gene is not expressed in the Streptomyces coelicolor bldA- mutant strain, indicating the importance of the bldA tRNA in its mRNA translation.
Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content (2008)

Top-down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic pathways that might be overcome by biotechnological approaches. Previous studies on lipid accumulation in oilseed rape have suggested that diacylglycerol acyltransferase (DGAT), which catalyses the final step in seed oil biosynthesis, might be an effective target for enhancing seed oil content. Here, increased seed oil content, increased DGAT activity, and reduced substrate:product ratio are demonstrated, as well as reduced flux control by complex lipid assembly, as determined by TDCA in Brassica napus (canola) lines which overexpress the gene encoding type-1 DGAT. Lines overexpressing DGAT1 also exhibited considerably enhanced seed oil content under drought conditions. These results support the use of TDCA in guiding the rational selection of molecular targets for oilseed modification. The most effective lines had a seed oil increase of 14%. Moreover, overexpression of DGAT1 under drought conditions reduced this environmental penalty on seed oil content.
Regulation of activation-induced receptor activator of NF-κB ligand (RANKL) expression in T cells (2002)

Receptor activator of NF-kappaB ligand (RANKL) is a type II membrane protein of the TNF family and plays a critical role in the regulation of osteoclastogenesis. RANKL expressed on osteoblastic stromal cells has been shown to support osteoclast differentiation originated from hematopoietic precursors. Interestingly, RANKL is also expressed on cells of the immune system including T cells and dendritic cells. We have shown that anti-CD3 could induce RANKL expression in T cell hybridoma A1.1 cells and splenic T cells. RANKL expressed on T cells could effectively induce osteoclast formation from the whole population of murine splenocytes. Furthermore, we have found that the induction of RANKL expression is solely dependent on TCR activation-induced Ca2+ mobilization since its expression can be blocked by cyclosporine A and TMB-8, a Ca2+ mobilization inhibitor. Additionally, treatment of A1.1 cells with ionomycin alone also strongly induces RANKL expression, while phorbol myristate acetate by itself does not. Moreover, although inhibition of c-myc has significant effects on anti-CD3-induced Fas ligand (FasL) expression, we have found that the anti-CD3-induced RANKL expression is independent of c-myc. Surprisingly, in contrast to its inhibitory effect on FasL expression, TGF-beta dramatically increased the expression of anti-CD3-induced RANKL expression. In addition to its potential role in immune responses, RANKL expressed on activated T lymphocytes may provide a mechanism for the communication between the immune and skeletal systems during immune responses and disease states such as rheumatoid arthritis.
Web Mining Overview (2005)

In the span of a decade, the World Wide Web has been transformed from a tool for information sharing among researchers into an indispensable part of everyday activities. This transformation has been characterized by an explosion of heterogeneous data and information available electronically, as well as increasingly complex applications driving a variety of systems for content management, e-commerce, e-learning, collaboration, and other Web services. This tremendous growth, in turn, has necessitated the development of more intelligent tools for end users as well as information providers in order to more effectively extract relevant information or to discover actionable knowledge.
Layout-Aware Switching Activity Localization to Enhance Hardware Trojan Detection (2011)

Government agencies and the semiconductor industry have raised serious concerns about malicious modifications to the integrated circuits. The added functionality known as hardware Trojan poses major detection and isolation challenges. This paper presents a new hardware trust architecture to magnify functional Trojans activity. Trojan detection resolution depends on Trojan activity directly and circuit activity reversely. The proposed architecture reorders scan cells based on their placement during physical design to reduce circuit switching activity by limiting it into a specific region. This helps magnify Trojan contribution to the total circuit transient power by increasing Trojan-to-circuit switching activity (TCA) and Trojan-to-circuit power consumption (TCP). The proposed technique aims to improve the efficiency of power-based side-channel signal analysis techniques for detecting hardware Trojans. Our simulation results demonstrate the efficiency of the method in significantly increasing TCA and TCP.