gebhart/patrepeval huggingface dataset:
Long-Term Function of Porcine Islets and Single Cells Embedded in Barium-Alginate Matrix (1993)

The influence of alginate-embedding on the maintenance of functioning and morphological integrity in long-term culture of isolated porcine islets and islet cells was studied. Free-floating islets and islet cells served for control. Function was tested after the 1st, 2nd and 4th week. Basal and glucose-stimulated insulin secretion of embedded islets decreased slightly, but significantly after the first week (from 4.39 +/- 0.64 to 2.87 +/- 0.47 at normal and from 11.96 +/- 1.44 to 4.76 +/- 0.78 microU pro 24 h pro islet at elevated glucose concentration, p < 0.05 and < 0.01, resp.) and remained unchanged thereafter. Glucose-stimulation resulted in significant increases in insulin secretion at all three testings (p < 0.001, < 0.01 and < 0.01). Single cells in alginate matrix had even more stable insulin secretion throughout the whole cultivation with significant increases to glucose challenge (p < 0.01, < 0.01 and < 0.05). In contrast, insulin secretion of free-floating islet cells decreased from 5.70 +/- 1.19 to 2.04 +/- 0.64 and to 1.05 +/- 0.33 at basal conditions (p < 0.01 and < 0.05) and from 11.39 +/- 1.87 to 2.76 +/- 0.76 and to 2.15 +/- 0.71 microU pro 24 h pro islet under stimulation (p < 0.01 and not sign). In addition, the secretory response to glucose challenge was significant only at the first testing (p < 0.05). Non-embedded islets could be tested only at the first week since after this time they dissociated to single cells. Embedded islets and single cells showed intact morphology after four weeks with trypan blue (TPB) positivity of less than 5%.(ABSTRACT TRUNCATED AT 250 WORDS)
Radial microstrip slotline feed network for circular mobile communications array (2002)

A planar circular array with endfire linearly tapered slot antenna (LTSA) elements [Simons and Lee, 1992] is described. The LTSA has several advantages which include high element gain, wide bandwidth, equal beam width in the two orthogonal planes by proper choice of flare angle and simple construction. The circular array radiates in the radial direction in the azimuth plane and hence eliminates the need for phase shifters and mechanical scanning hardware. To achieve the elevation angle a ground plane is placed below the array which causes the beam to tilt above the horizon. The ground plane also simulates the metal roof top of vehicles. The feed network consists of a sixteen way radial power splitter which is electromagnetically coupled to the LTSA circular array. Electromagnetic coupling eliminates pin and solder connections and therefore improves reliability and lowers the cost. It also results in a wider bandwidth. This type of feed network is very simple and therefore has potential applications in several other configurations of planar microstrip patch arrays also.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Path planning algorithm for autonomous mobile robot in dynamic environment (2015)

Mobile robots are mostly used in many industrial and military applications. Researches in the path planning is one of the most important aspects in mobile robot researches. Path planning for a mobile robot need to find a collision free path through the robot's environment with obstacles from the specified start location to a desired (target) goal location while satisfying certain optimization conditions. Existing path planning methods like graphical methods such as visibility graph, the potential field and the cell decomposition are designed for static environments, in which there are stationary obstacles. In practical systems such as Marine Science Research, Robots in Industry, and military combat applications, robots usually face dynamic environments where both moving and stationary obstacles exist. This project proposes a heuristic based method to search the feasible initial path efficiently. The heuristic based method is then combined into the simulated annealing algorithm based approach for dynamic robot path planning. Therefore the quality of the solution is characterised by the length of the planned path and it is improved with the combined heuristic method in the simulated annealing based approach for both runtime and offline path planning.
SRI's experience with the ATIS evaluation (1990)

SRI International participated in the June 1990 Air Travel Information System (ATIS) natural-language evaluation. This report briefly describes the system that SRI used in the evaluation, analyzes SRI's results, and makes some recommendations for changes in the database structure and data collection system to be used for future ATIS evaluations.
Thermally excited ferromagnetic resonance as diagnostic tool for spin valve heads (2003)

Thermally excited ferromagnetic resonance (TEFMR) in a spin valve head gives rise to mag-noise while its power spectral density directly reflects the magnetic field distribution in the sensing layer as well as the corresponding micromagnetic states. In this article, we report an experimental study on utilization of TEFMR spectral characteristics for spin valve head diagnosis and characterization. The study suggests that the variation of the micromagnetic states in the PM layer at the junctions is one of the most important factors causing the head-to-head variations of the recording performance, especially the heads from the same wafer.
Isolation and Initial Application of a Novel Peptide That Specifically Recognizes the Neural Stem Cells Derived from Rhesus Monkey Embryonic Stem Cells (2010)

The search for new receptor ligands is important in the study of embryonic stem (ES) cell differentiation processes. In this study, a novel peptide (HGEVPRFHAVHL) with a specific ability to bind with neural stem cells derived from rhesus monkey ES cells was successfully screened out using a Ph.D-12 peptide phage display library. High affinity and specificity of the HGE phage were shown in an enzyme-linked immunosorbent assay. The binding ability of the phage could be matched with that of a chemically synthesized peptide with a sequence identical to that displayed by the phage, indicating that this binding capability manifests a peptide sequence. Combined with quantum dots, the HGE peptide can be used as a direct tool to show optical imaging of specific binding on a single cell membrane. Further results of Western blot showed that the HGE peptide interacted with 48/34-kDa proteins on the membrane of neural stem cells. This work is the first time that a phage display technique has been applied in ES cell differentiation studies. The findings extend the utilization of a targeting agent for neural stem cells and can also be used as a research tool in studying other cell lineages derived from ES cells.
Solubility of Polymers and Copolymers in Supercritical CO2 (1996)

Cloud-point data to temperatures of 270 °C and 3000 bar are presented for CO2 with the family of poly(acrylates) including methyl, ethyl, propyl, butyl, ethylhexyl, and octadecyl, with poly(butyl methacrylate), with poly(vinyl acetate), with statistically random copolymers of poly(ethylene-co-methyl acrylate) with 41, 31, and 18 mol % acrylate, with poly(tetrafluoroethylene-co-hexafluoropropylene) and poly(vinylidene-co-hexafluoropropylene) copolymers, each with ∼20 mol % hexafluoropropylene, and with Teflon AF. Over the same range of conditions, CO2 cannot dissolve polyethylene, poly(acrylic acid), poly(methyl methacrylate), poly(ethyl methacrylate), polystyrene, poly(vinyl fluoride), or poly(vinylidene fluoride). CO2 is a weak solvent that exhibits the temperature-sensitive characteristics observed with polar solvents. The solubility of a nonpolar hydrocarbon polymer or a copolymer in CO2 can be increased by at least partially fluorinating the polymer or by incorporating some polar groups into the backbone architecture of the polymer. Because it is such a weak solvent, CO2 can distinguish differences in polymer architecture even for polymers from the same chemical family, which means that polymer free volume plays a role in determining solubility.
Properties of carbon ion deposited tetrahedral amorphous carbon films as a function of ion energy (1996)

Ion energy, controlled by the substrate bias, is an important parameter in determining properties of films deposited by the filtered cathodic vacuum arc technique. The substrate bias determines the ion energy distribution of the growth species. The ion energy is varied, while keeping the other deposition conditions constant, in order to study the effect of ion energy on the film properties. The films were characterized by their optical and mechanical parameters using an ellipsometer, surface profilometer, optical spectrometer, and nanoindenter. Electron energy-loss spectroscopy and Raman spectroscopy were used for structural analysis of the films.
Synthesis and some transformations of 5‐benzotriazolyl‐3,4‐dihydropyrid‐2‐ones (1996)

Abstract 5‐(Benzotriazolyl)‐substituted 3,4‐dihydropyrid‐2‐ones were obtained by condensation of 4‐benzo‐triazolyl‐5‐phenyl(methyl)‐5‐oxopentanoic acids with benzyl or aryl amines. Dehydrogenation of 3,4‐dihydropyrid‐2‐ones resulted in 5‐benzotriazolyl‐substituted pyrid‐2‐ones and/or 5‐(phenylamino)pyrid‐2‐ones, products of nitrogen elimination from the benzotriazolyl substituent. In turn, 5‐benzotriazol‐1‐yl‐substituted pyrid‐2‐ones eliminated nitrogen under Graebe‐Ullmann conditions to give indolopyridones.
15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-β-induced suppressor of human gastrointestinal cancers (2004)

Marked increased expression of cyclooxygenase 2 (COX-2), a prostaglandin-synthesizing enzyme that is pharmacologically inhibited by nonsteroid anti-inflammatory-type drugs, is a major early oncogenic event in the genesis of human colon neoplasia. We report that, in addition to inducing expression of COX-2, colon cancers further target the prostaglandin biogenesis pathway by ubiquitously abrogating expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2. We find that 15-PGDH transcript and protein are both highly expressed by normal colonic epithelia but are nearly undetectable in colon cancers. Using gene transfection to restore 15-PGDH expression in colon cancer cells strongly inhibits the ability of these cells to form tumors in immune-deficient mice and demonstrates 15-PGDH to have functional colon cancer tumor suppressor activity. In interrogating the mechanism for 15-PGDH expression loss in colon cancer, we determined that colonic 15-PGDH expression is directly controlled and strongly induced by activation of the TGF-beta tumor suppressor pathway. These findings thus delineate an enzymatic pathway that induces colon cancer suppression, a pathway that is activated by TGF-beta and mediated by 15-PGDH.
Bioinspired Oil Strider Floating at the Oil/Water Interface Supported by Huge Superoleophobic Force (2012)

Oil pollution to aquatic devices, especially to those oil-cleaning devices and equipment-repairing robots during oil spill accidents, has drawn great attention and remains an urgent problem to be resolved. Developing devices that can move freely in an oil/water system without contamination from oil has both scientific and practical importance. In nature, the insect water strider can float on water by utilizing the superhydrophobic supporting force received by its legs. Inspired by this unique floating phenomenon, in this article, we designed a model device named "oil strider" that could float stably at the oil/water interface without contamination by oil. The floating capability of the oil strider originated from the huge underwater superoleophobic supporting force its "legs" received. We prepared the micro/nanohierarchical structured copper-oxide-coated copper wires, acting as the artificial legs of oil strider, by a simple base-corrosion process. The surface structures and hydrophilic chemical components of the coatings on copper wires induced the huge superoleophobic force at the oil/water interface, to support the oil strider from sinking into the oil. Experimental results and theoretical analysis demonstrate that this supporting force is mainly composed of three parts: the buoyancy force, the curvature force, and the deformation force. We anticipate that this artificial oil strider will provide a guide for the design of smart aquatic devices that can move freely in an oil/water system with excellent oil repellent capability, and be helpful in practical situations such as oil handling and oil spill cleanup.
Efficacy of the Herpes Simplex Virus Types 1 and 2 Mutant Viruses to Confer Protection against Zosteriform Spread in Mice (1993)

Two mutant viruses, HSV-2 XD192 and HSV-1 1716, failed to generate zosteriform lesions when injected in high dose into BALB/c and C3H mice. Mice exposed to mutant viruses were solidly immune to challenge by wild-type homologous or heterologous virus. However, at lower immunizing doses protection was evident against lethality, but not skin lesions, especially in the case of mutant XD192. Protection could be conferred with lymphoid cells from mutant virus immune mice and again, protection against lethality was more frequent than prevention of skin lesions. On the basis of cell fractionation studies, protection against lethality was assumed to be principally the function of CD8+ T lymphocytes. The implications of the results in terms of vaccine development were briefly discussed.
INTRAGRAFT ACTIVATION OF GENES ENCODING CYTOTOXIC T LYMPHOCYTE EFFECTOR MOLECULES PRECEDES THE HISTOLOGICAL EVIDENCE OF REJECTION IN HUMAN CARDIAC TRANSPLANTATION1 (2001)

The assessment of intragraft levels of cytotoxic T lymphocyte effector molecule mRNA represents a valuable tool in the monitoring of cardiac allograft rejection, especially considering the predictive value for warning of impending acute rejection.
[The analysis on the hepatitis B awareness and factors influencing of reproductive women in poverty rural areas of Gansu province]. (2009)

The HB awareness of women of childbearing age was low. There was discrimination to the HB virus infected people. It is necessary to conduct various health education among different people.
Structure-activity relationships of antibacterial 6,7- and 7,8-disubstituted 1-alkyl-1,4-dihydro-4-oxoquinoline-3-carboxylic acids (1980)

Previous quantitative and qualitative structure-activity studies in antibacterial monosubstituted 1-ethyl-1,4-dihydro-4-oxoquinoline-3-carboxylic acids prompted us to synthesize the 6,7,8-polysubstituted compounds. In this paper, the preparation and antibacterial activity of the 6,7- and 7,8-disubstituted compounds and their derivatives are described. Among these compounds, 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)quinoline-3-carboxylic acid (34) possessed many significant activities and was more active than oxolinic acid (84) against Gram-positive and Gram-negative bacteria. Structure-activity relationships are discussed.
Synthesis and structure-activity studies of N,N'-diarylguanidine derivatives. N-(1-naphthyl)-N'-(3-ethylphenyl)-N'-methylguanidine: a new, selective noncompetitive NMDA receptor antagonist (1994)

Diarylguanidines, acting as NMDA receptor ion channel site ligands, represent a new class of potential neuroprotective drugs. Several diarylguanidines structurally related to N,N'-di-o-tolylguanidine (DTG), a known selective sigma receptor ligand, were synthesized and evaluated in in vitro radioligand displacement assays, with rat or guinea pig brain membrane homogenates, using the NMDA receptor ion channel site specific radioligand [3H]-(+)-5(S)-methyl-10(R),11-dihydro-5H-dibenzo[a,d]cyclohepten-5 ,10- imine (MK-801, 3), and the sigma receptor-specific radioligand [3H]-di-o-tolylguanidine (DTG, 5). This paper presents the structure-activity relationships leading to novel tri- and tetrasubstituted guanidines, which exhibit high selectivity for NMDA receptor ion channel sites and weak or negligible affinity for sigma receptors. The in vitro binding results from symmetrically substituted diphenylguanidines indicated that compounds having ortho or meta substituents (with respect to the position of the guanidine nitrogen) on the phenyl rings showed greater affinity for the NMDA receptor ion channel site compared with para-substituted derivatives. Among the group of ring substituents studied for symmetrical diarylguanidines, an isopropyl group was preferred at the ortho position and an ethyl group was preferred at the meta position. Several unsymmetrical guanidines containing a naphthalene ring on one nitrogen atom and an ortho- or a meta-substituted phenyl ring on the second nitrogen atom, e.g., N-1-naphthyl-N'-(3-ethylphenyl)guanidine (36), showed a 3-5-fold increase in affinity for the NMDA receptor ion channel site and no change in sigma receptor affinity compared to the respective symmetrical counterparts. Additional small substituents on the guanidine nitrogen atoms bearing the aryl rings resulted in tri- and tetrasubstituted guanidine derivatives which retained affinity for NMDA receptor ion channel sites but exhibited a significant reduction in their affinities for sigma receptors. For example, N-1-naphthyl-N'-(3-ethylphenyl)-N'-methylguanidine (40) showed high affinity for the NMDA receptor ion channel site (IC50 = 36 nM vs [3H]-3) and low affinity for sigma receptors (IC50 = 2540 nM vs [3H]-5). Selectivity for the NMDA receptor ion channel sites over sigma receptors appears to be dependent upon the structure of the additional substituents on the guanidine nitrogen atoms bearing the aryl groups. Methyl and ethyl substituents are most preferred in the tri- and tetrasubstituted diarylguanidines. The trisubstituted guanidine, N-1-naphthyl-N'-(3-ethylphenyl)-N'-methylguanidine (40) and its close analogues showed good in vivo neuroprotection and are potential neuroprotective drug candidates for the treatment of stroke and other neurodegenerative disorders.
Cutting Edge: Cyclooxygenase-2 Activation Suppresses Th1 Polarization in Response to Helicobacter pylori (2003)

Helicobacter pylori infection causes a Th1-driven mucosal immune response. Cyclooxygenase (COX)-2 is up-regulated in lamina propria mononuclear cells in H. pylori gastritis. Because COX-2 can modulate Th1/Th2 balance, we determined whether H. pylori activates COX-2 in human PBMCs, and the effect on cytokine and proliferative responses. There was significant up-regulation of COX-2 mRNA and PGE(2) release in response to H. pylori preparations. Addition of COX-2 inhibitors or an anti-PGE(2) Ab resulted in a marked increase in H. pylori-stimulated IL-12 and IFN-gamma production, and a decrease in IL-10 levels. Addition of PGE(2) or cAMP, the second messenger activated by PGE(2), had the opposite effect. Similarly, stimulated cell proliferation was increased by COX-2 inhibitors or anti-PGE(2) Ab, and was decreased by PGE(2). Our findings indicate that COX-2 has an immunosuppressive role in H. pylori gastritis, which may protect the mucosa from severe injury, but may also contribute to the persistence of the infection.
A Mutant at Position 87 of the GroEL Chaperonin Is Affected in Protein Binding and ATP Hydrolysis (1995)

The highly conserved aspartic acid residue at position 87 of the Escherichia coli chaperonin GroEL was mutated to glutamic acid. When expressed in an E. coli groEL mutant strain deficient for phage morphogenesis, plasmid-encoded GroEL mutant D87E restored T4 phage morphogenesis. It did not, however, reactivate the transcription of a recombinant luciferase operon from Vibrio fischeri. In vitro, GroEL mutant D87E was found to be impaired in the ability to bind nonnative proteins and to hydrolyze ATP, resulting in less efficient refolding of urea-denatured ribulose-1,5-bisphosphate carboxylase/oxygenase. Mutant oligomer D87E GroEL14 was able to bind GroES7 as efficiently as wild-type GroEL14. The conserved aspartic acid residue at position 87 located in the equatorial domain of GroEL (Braig, K., Otwinowski, Z., Hegde, R., Boisvert, D.C., Joachimiak, A., Horwich, A.L., and Sigler, P.B. (1994) Nature 371, 578-586) is thus inferred to have a dual effect on the binding of nonnative proteins to the GroEL14 core chaperonin and on ATP hydrolysis.
Bordatella pertussisRespiratory Infection in Children Is Associated with Preferential Activation of Type 1 T Helper Cells (1997)

The mechanism of protective immunity against Bordetella pertussis generated following recovery from whooping cough in childhood has not yet been elucidated. Studies with a murine respiratory infection model have indicated that cellular immunity, mediated by Th1 cells, plays a role in the clearance of a primary infection with B. pertussis and in protection against subsequent challenge. In the present study, the induction of B. pertussis-specific Th cell subsets in children was examined. Peripheral blood mononuclear cells from B. pertussis-infected or convalescent children proliferated and secreted cytokines following antigen stimulation in vitro. In contrast, responses were weak or undetectable in the majority of children who had not been infected or vaccinated. In all cases, responding T cells produced interferon-gamma but low or undetectable interleukin-5. The findings suggest that Th1 cells may play a role in protective immunity generated following infection with B. pertussis in children.
Photoaffinity radioligand for NADH:ubiquinone oxidoreductase: [S-C3H2](trifluoromethyl)diazirinyl-pyridaben (1998)

Pyridaben is a new and very potent insecticide and miticide that acts by inhibiting the activity of NADH:ubiquinone oxidoreductase (the most complex of all the respiratory enzymes). The binding site, presumed to be the same as that of rotenone and fenazaquin, resides at an unknown location within the 43-polypeptide-subunit Complex I. To define the structure of the pyridaben-inhibition site(s), we prepared [S-C3H2](trifluoromethyl)diazirinyl-pyridaben as a photoaffinity probe. Tritium was incorporated by reducing 4-(trifluoromethyl)diazirinylphenylacetyl fluoride to the benzyl alcohol with freshly prepared LiB3H4 at 97% tritium enrichment. The tritium-labeled alcohol was converted to the benzyl bromide derivative and coupled to 2-tert-butyl-4-chloro-5-mercapto-3(2H)-pyridazinone to obtain the photoaffinity probe (56 Ci/mmol) with an IC50 of 3.0 nM for NADH:ubiquinone oxidoreductase activity of bovine heart electron transport particles. [S-C3H2](Trifluoromethyl)diazirinyl-pyridaben is an improved photoaffinity radioligand combining outstanding potency for inhibiting NADH:ubiquinone oxidoreductase activity, high specific activity close to the theoretical value, and a preferred photolabile substituent (known to combine high reactivity and generation of a carbene species at wavelengths not damaging to proteins). © 1998 John Wiley & Sons, Ltd.
200mm GaN-on-Si epitaxy and e-mode device technology (2015)

In this work, three types of high-voltage buffer architectures for GaN-on-200mm-Si epitaxy are compared and discussed. Two device architectures, recessed gate MISHEMTs and p-GaN HEMTs technology, to obtain e-mode operation developed on these buffers are also discussed. Threshold voltage/output current tuning, threshold voltage stability and possible issues are highlighted. A possible device architecture that combines the best of the two approaches is proposed together with preliminary test results.