gebhart/patrepeval huggingface dataset:
PCR mediated detection of a new human receptor-tyrosine-kinase, HEK 2. (1993)

We have previously amplified cDNA subfragments of protein-tyrosine-kinases (PTKs) by using the polymerase chain reaction (PCR) and specific sets of oligonucleotide primers derived from nucleotide sequences of their kinase domain. In this study we have used a more directed approach to identify new members of the EPH/elk-family by PCR of human embryonic cDNA: we utilized oligonucleotide primers specifically designed to a highly conserved N-terminal motif and the kinase region of EPH/elk-PTKs in RNA-PCRs. The 5' and 3' elongation of the primary PCR product was achieved by the RACE (rapid amplification of cDNA ends)-technique. Sequence analysis of 3.8 kb of overlapping PCR products allowed to identify a novel receptor-PTK, HEK 2 (human embryo kinase 2), as an additional member of this family, without the need to screen a cDNA library. This approach should be useful for the rapid isolation of other PTK-genes as well. Analysis of genomic DNA placed HEK 2 on chromosome 3. Northern blot analysis demonstrated the expression of a 4.6 kb HEK 2-mRNA in lung, brain, pancreas, liver, placenta, kidney, skeletal muscle, heart and several human cells. In a protein kinase assay with HEK 2-specific immunoprecipitates from the human epidermoid carcinoma cell line A431, a protein of 130 kDa was found phosphorylated.
Voriconazole: A New Triazole Antifungal Agent (2003)

Voriconazole is a second-generation azole antifungal agent that shows excellent in vitro activity against a wide variety of yeasts and molds. It can be given by either the intravenous or the oral route; the oral formulation has excellent bioavailability. The side effect profile of voriconazole is unique in that non-sight-threatening, transient visual disturbances occur in approximately 30% of patients given the drug. Rash (which can manifest as photosensitivity) and hepatitis also occur. The potential for drug-drug interactions is high and requires that careful attention be given to dosage regimens and monitoring of serum levels and effects of interacting drugs. Voriconazole has been approved for the treatment of invasive aspergillosis and refractory infections with Pseudallescheria/Scedosporium and Fusarium species, and it will likely become the drug of choice for treatment of serious infections with those filamentous fungi.
Degradation of extracellular-matrix proteins by human cathepsin B from normal and tumour tissues (1992)

Our laboratory has previously demonstrated that increased malignancy of several histological types of human and animal tumours is associated with increases in their cathepsin B activity, particularly cathepsin B activity associated with plasma-membrane/endosomal vesicles or shed vesicles. Here we report that cathepsin B from normal or tumour tissues degrades purified extracellular-matrix components, type IV collagen, laminin and fibronectin, at both acid pH and neutral pH. The number and sizes of degradation products were analysed by SDS/PAGE. Cathepsin B from both sources exhibited similar activities towards, and similar patterns of cleavage of, the extracellular-matrix proteins. At neutral pH, cathepsin B from both sources appeared to undergo autodegradation, a process that was decreased in the presence of alternative substrates such as the extracellular-matrix proteins. Cathepsin B readily degraded type IV collagen at 25 degrees C, indicating activity towards native type IV collagen. Fibronectin degradation products of 100-200 kDa and of 18 and 22 kDa were observed. A single 70 kDa fragment was released from laminin under non-reducing conditions and multiple fragments ranging from 45 to 200 kDa under reducing conditions. These results suggest that cathepsin B at or near the surface of malignant tumour cells may play a functional role in the focal dissolution of extracellular matrices.
The Feasibility Study of Non-Invasive Fetal Trisomy 18 and 21 Detection with Semiconductor Sequencing Platform (2014)

These results provide the evidence that fetal trisomy 18 and 21 detection can be performed with semiconductor sequencer. Our data also suggest that a prospective study should be performed with a larger cohort of clinically diverse obstetrics patients.
The Vacuum Assisted Closure Device (2002)

The VAC provides a safe and effective method for securing STSGs and is associated with improved graft survival as measured by a reduction in number of repeated STSGs.
Microcin MccPDI reduces the prevalence of susceptible Escherichia coli in neonatal calves (2014)

MccPDI-producing E. coli strongly inhibit pathogenic E. coli strains in vitro. We show herein that MccPDI functions in vivo, and thus, these strains may be candidate probiotics against pathogenic strains of E. coli.
The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region. (1991)

The human foamy virus (HFV) genome possesses three open reading frames (bel 1, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most likely candidate to encode the viral transactivator. Results presented here confirmed this and showed further that a deletion introduced only into the bel 1 open reading frame of a plasmid derived from an infectious molecular clone of HFV abolished transactivation. In contrast, deletions in bel 2 and bel 3 had only minor effects on the ability to transactivate. The role of the bel 1 genomic region as a transactivator was further investigated by eukaryotic expression of a genome fragment of HFV spanning the bel 1 open reading frame. A construct expressing bel 1 under control of a heterologous promoter was found to transactivate the HFV long terminal repeat in a dose-dependent fashion. Furthermore, it is shown that the U3 region of the HFV long terminal repeat is sufficient to respond to the HFV transactivator.
Life span of naive and memory t cells (1995)

The life span of mature T cells is reviewed. Peripheral T lymphocytes are a heterogeneous population and comprise a mixture of naive, effector and memory cells. The recirculating pool of mature T cells is formed during young life through gradual release of naive T cells from the thymus. In adults, the pool of mature T cells is relatively self-sufficient, and input of new T cells from the thymus declines to low levels. Studies on T cell turnover indicate that most peripheral T cells can remain in a resting state for long periods (months in rodents and years in humans). Examination of the phenotype of dividing versus nondividing cells suggests that typical naive T cells are long-lived resting cells whereas the majority of effector and memory T cells have a much more rapid turnover. However, some memory T cells appear to divide very infrequently and eventually return to a resting state. The factors controlling the generation and maintenance of memory T cells are discussed.
Development of New Semiconducting Polymers for High Performance Solar Cells (2008)

A new low band gap semiconducting polymer, PTB1, was synthesized and found promising for solar energy harvesting. Simple polymer solar cells based on PTB1 and methanofullerene [6,6]-phenyl-C(71)-butyric acid methyl esters (PC(71)BM) exhibit a solar conversion efficiency of 5.6%. An external quantum efficiency of 67% and fill-factor of 65% are achieved, both of which are among the highest values reported for a solar cell system based on a low band gap polymer.
Structure and dynamics of silica aerogels (1992)

Abstract The current status of our experiments on fractal silica aerogels is reviewed. Remarks are made concerning the direct space observations of the structure compared with reciprocal space measurements. The scaling of the intensities observed in the Raman scattering from fractons is analysed in the light of recent theoretical progess. The origin of, and evidence for, an elasticity cross-over in the fracton frequency range are discussed. The fracton dimension ᵭ is clearly shown to be an additional dimension to characterize the fractal rather than a universal quantity.
Polymorphism at codon 54 of mannose-binding protein gene influences AIDS progression but not HIV infection in exposed children (1999)

The present study shows the polymorphism at codon 54 of the MBP gene investigated by amplificationrefractory mutation system–polymerase chain reaction (ARMS–PCR) in 52 children with perinatal HIV-1 infection. In this cohort, the time of infection exposure was precisely determined because of vertical transmission at delivery.
Mannoproteins fromCryptococcus neoformansPromote Dendritic Cell Maturation and Activation (2005)

Our previous data show that mannoproteins (MPs) from Cryptococcus neoformans are able to induce protective responses against both C. neoformans and Candida albicans. Here we provide evidence that MPs foster maturation and activation of human dendritic cells (DCs). Maturation was evaluated by the ability of MPs to facilitate expression of costimulatory molecules such as CD40, CD86, CD83, and major histocompatibility complex classes I and II and to inhibit receptors such as CD14, CD16, and CD32. Activation of DCs was measured by the capacity of MPs to promote interleukin-12 and tumor necrosis factor alpha secretion. DC-induced maturation and interleukin-12 induction are largely mediated by engagement of mannose receptors and presume MP internalization and degradation. DC activation leads to IkappaBalpha phosphorylation, which is necessary for nuclear factor kappaB transmigration into the nucleus. MP-loaded DCs are efficient stimulators of T cells and show a remarkable capacity to promote CD4 and CD8 proliferation. In conclusion, we have evidenced a novel regulatory role of MPs that promotes their candidacy as a vaccine against fungi.
Efficient Photoluminescence and Electroluminescence from a Soluble Polyfluorene (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEfficient Photoluminescence and Electroluminescence from a Soluble PolyfluoreneQibing Pei and YangView Author Information UNIAX Corporation, 6780 Cortona Drive Santa Barbara, California 93117 Cite this: J. Am. Chem. Soc. 1996, 118, 31, 7416–7417Publication Date (Web):August 7, 1996Publication History Received7 May 1996Published online7 August 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja9615233https://doi.org/10.1021/ja9615233rapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views5922Altmetric-Citations737LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Conjugated polymers,Degradation,Light,Polymers,Thin films Get e-Alerts
Dance Band on the Titanic (2002)

Biomechanical signaling is a complex interaction of both intracellular and extracellular components. Both passive and active components are involved in the extracellular environment to signal through specific receptors to multiple signaling pathways. This review provides an overview of extracellular matrix, specific receptors, and signaling pathways for biomechanical stimulation in cardiac hypertrophy.
OctNet: Learning Deep 3D Representations at High Resolutions (2017)

We present OctNet, a representation for deep learning with sparse 3D data. In contrast to existing models, our representation enables 3D convolutional networks which are both deep and high resolution. Towards this goal, we exploit the sparsity in the input data to hierarchically partition the space using a set of unbalanced octrees where each leaf node stores a pooled feature representation. This allows to focus memory allocation and computation to the relevant dense regions and enables deeper networks without compromising resolution. We demonstrate the utility of our OctNet representation by analyzing the impact of resolution on several 3D tasks including 3D object classification, orientation estimation and point cloud labeling.
Hemorrhage associated with pelvic fractures: causes, diagnosis, and emergent management. (1991)

The high risk of exsanguinating hemorrhage in patients with pelvic ring disruption demands aggressive, yet balanced orthopedic and angiographic management as soon as patients are admitted to the emergency department. We present a perspective of our experience in two trauma centers and propose a logical approach to early prediction, diagnosis, and management of hemorrhage associated with pelvic fractures. Our method is based on knowledge of pelvic anatomy and an understanding of the mechanisms of injury and their wounding capacity, given that the mechanism of injury determines the type of pelvic ring disruption and that the probability of arterial hemorrhage is--to a great extent--a function of the type of pelvic fracture. The risks of diagnostic peritoneal lavage and of excessive radiologic studies of noncritical injuries are emphasized. The principles guiding arterial embolization and the application of external fixators are discussed.
Use of Modified Free Energy Theorems to Predict Equilibrium Growing and Etching Shapes (1962)

This paper offers a construction that gives the limiting or ``equilibrium'' shape of an etching crystal. The conditions for hillock and pit stability and face rounding are also obtained. Experimentally, germanium cylinders with different zone axes were etched to develop ``equilibrium cross sections.'' These were in good agreement with the construction. Other uses of the construction are discussed.
Graphene-Based Quantum Capacitance Wireless Vapor Sensors (2014)

A wireless vapor sensor based on the quantum capacitance effect in graphene is demonstrated. The sensor consists of a metal-oxide-graphene variable capacitor (varactor) coupled to an inductor, creating a resonant oscillator circuit. The resonant frequency is found to shift in proportion to water vapor concentration for relative humidity (RH) values ranging from 1% to 97% with a linear frequency shift of 5.7 kHz/%RH ± 0.3 kHz/%RH. The capacitance values extracted from the wireless measurements agree with those determined from capacitance-voltage measurements, providing strong evidence that the sensing arises from the variable quantum capacitance in graphene. These results represent a new sensor transduction mechanism and pave the way for graphene quantum capacitance sensors to be studied for a wide range of chemical and biological sensing applications.
Combining Antibody-Directed Presentation of IL-15 and 4-1BBL in a Trifunctional Fusion Protein for Cancer Immunotherapy (2013)

Influencing the cytokine receptor network that modulates the immune response holds great potential for cancer immunotherapy. Although encouraging results have been obtained by focusing on individual members of the common γ-chain (γc) receptor family and TNF receptor superfamily so far, combination strategies might be required to further improve the effectiveness of the antitumor response. Here, we propose the combination of interleukin (IL)-15 and 4-1BBL in a single, tumor-directed molecule. Therefore, a trifunctional antibody fusion protein was generated, composed of a tumor-specific recombinant antibody, IL-15 linked to a fragment of the IL-15Rα chain (RD) and the extracellular domain of 4-1BBL. In soluble and targeted forms, the trifunctional antibody fusion protein RD_IL-15_scFv_4-1BBL was shown to stimulate activated T-cell proliferation and induce T-cell cytotoxicity to a similar degree as the bifunctional scFv_RD_IL-15 fusion protein. On the other hand, in targeted form, the trifunctional fusion protein was much more effective in inducing T-cell proliferation and IFN-γ release of unstimulated peripheral blood mononuclear cells (PBMC). Here, the additional signal enhancement could be attributed to the costimulatory activity of 4-1BBL, indicating a clear benefit for the simultaneous presentation of IL-15 and 4-1BBL in one molecule. Furthermore, the trifunctional antibody fusion protein was more effective than the corresponding bifunctional fusion proteins in reducing metastases in a tumor mouse model in vivo. Hence, the targeted combination of IL-15 and 4-BBL in the form of a trifunctional antibody-fusion protein is a promising new approach for cancer immunotherapy.
A study of Bi-Pb-Sr-Ca-Cu-O/Ag tape prepared by the jelly-roll process (1994)

We combined the electrophoretic deposition technique and the jelly-roll process to fabricate Bi-Pb-Sr-Ca-Cu-O (BPSCCO)/Ag tapes. A thick film of BPSCCO on an Ag substrate was prepared by electrophoretic deposition. The BPSCCO/Ag sheet was rolled up to the jelly-roll configuration and then cold rolled to form tape with six superconducting layers. The critical current density of the tape determined by magnetic measurement at 5 K in a 0.2 T field was about 6.5*104 A cm-2 and decreased to 1.3*104 A cm-2 near 5 T.
Vaccines in Civilian Defense Against Bioterrorism (1999)

In the United States, over the past half century, we have lived under the protective umbrella of vaccination programs that shield our population from a dozen serious and sometimes fatal naturally transmitted illnesses. Vaccination has been the single most cost-effective public health intervention. However, the value of vaccines in protecting the population against the deliberate release of infectious organisms is not so clear-cut. The U.S. armed forces have recognized the military value of vaccines against biological threats and have a long-standing research and development program for a series of vaccines to protect service members from hostile use of a biological agent. Vaccination against anthrax is under way in all three armed services. The Department of Defense has a large program to develop and license additional vaccines for biological defense. For the military, vaccination is an effective means of countering a known threat because the population at risk is easily defined and a high level of vaccine coverage can be achieved. In evaluating the role of vaccines for protecting the civilian population, quite different answers are reached. Despite the protective efficacy of vaccines against individual organisms, the very high costs and the great difficulties involved in vaccinating large populations, along with the broad spectrum of potential agents, make it impossible to use vaccines to protect the general population against bioterrorism. Thus, vaccines cannot be considered a first line of defense against bioterrorism for the general population, as they can be for the relatively small military population. However, if suitable vaccines can be made available, they have several potential uses: control of a smallpox epidemic and prevention of a global pandemic, postexposure prophylaxis against anthrax (with antibiotics), and preexposure prophylaxis in first-responders at high risk, laboratory workers, and health-care providers. Smallpox and anthrax, which pose the greatest risk for causing large numbers of casualties in the event of an effective release by a terrorist group, are at the top of the list of threat agents. Licensed vaccines against both anthrax and smallpox that protect against aerosol transmission are available. An existing licensed plague vaccine is protective against fleatransmitted disease but not against aerosol challenge in animal experiments or against pneumonic plague. This vaccine is in limited supply, and the manufacturer has recently ceased production. The Department of Defense Joint Vaccine Acquisition Program has several experimental vaccines in development (Table). These vaccines will be further developed and tested with the intent of obtaining products licensed by the U.S. Food and Drug Administration.
New cache designs for thwarting software cache-based side channel attacks (2007)

Software cache-based side channel attacks are a serious new class of threats for computers. Unlike physical side channel attacks that mostly target embedded cryptographic devices, cache-based side channel attacks can also undermine general purpose systems. The attacks are easy to perform, effective on most platforms, and do not require special instruments or excessive computation power. In recently demonstrated attacks on software implementations of ciphers like AES and RSA, the full key can be recovered by an unprivileged user program performing simple timing measurements based on cache misses. We first analyze these attacks, identifying cache interference as the root cause of these attacks. We identify two basic mitigation approaches: the partition-based approach eliminates cache interference whereas the randomization-based approach randomizes cache interference so that zero information can be inferred. We present new security-aware cache designs, the Partition-Locked cache (PLcache) and Random Permutation cache (RPcache), analyze and prove their security, and evaluate their performance. Our results show that our new cache designs with built-in security can defend against cache-based side channel attacks in general-rather than only specific attacks on a given cryptographic algorithm-with very little performance degradation and hardware cost.
Managing infrastructure EMC (2009)

Integration is the term given to ensuring that the different elements of an electrified railway operate together to achieve the desired result. It is particularly necessary where interfaces exist between the high power traction units or electrification distribution system, and the low power signalling and communications control systems. Railway authorities in the past have found difficulty in implementing a management strategy that will ensure railway electrical systems are integrated. New railway projects and the privatisation of railways tend to divide the design process and contracts into separate engineering disciplines. This presentation seeks to explain the challenge of integrating the railway and the difficulties of determining suitable procurement arrangements commonly experienced in rail projects. Additionally it will demonstrate how railway authorities are being challenged by this difficult task of integrating electrical systems. The challenge of integrating the electrical systems will not be made any easier as client and public expectations of new rail transit systems require the railway designers to incorporate more versatile and sophisticated electrified railway and tram networks. (27 pages)