gebhart/patrepeval huggingface dataset:
The cell cycle-dependent localization of the CP190 centrosomal protein is determined by the coordinate action of two separable domains. (1995)

CP190, a protein of 1,096 amino acids from Drosophila melanogaster, oscillates in a cell cycle-specific manner between the nucleus during interphase, and the centrosome during mitosis. To characterize the regions of CP190 responsible for its dynamic behavior, we injected rhodamine-labeled fusion proteins spanning most of CP190 into early Drosophila embryos, where their localizations were characterized using time-lapse fluorescence confocal microscopy. A single bipartite 19-amino acid nuclear localization signal was detected that causes nuclear localization. Robust centrosomal localization is conferred by a separate region of 124 amino acids; two adjacent, nonoverlapping fusion proteins containing distinct portions of this region show weaker centrosomal localization. Fusion proteins that contain both nuclear and centrosomal localization sequences oscillate between the nucleus and the centrosome in a manner identical to native CP190. Fusion proteins containing only the centrosome localization sequence are found at centrosomes throughout the cell cycle, suggesting that CP190 is actively recruited away from the centrosome by its movement into the nucleus during interphase. Both native and bacterially expressed CP190 cosediment with microtubules in vitro. Tests with fusion proteins show that the domain responsible for microtubule binding overlaps the domain required for centrosomal localization. CP60, a protein identified by its association with CP190, also localizes to centrosomes and to nuclei in a cell cycle-dependent manner. Experiments in which colchicine is used to depolymerize microtubules in the early Drosophila embryo demonstrate that both CP190 and CP60 are able to attain and maintain their centrosomal localization in the absence of microtubules.
Ferric carboxymaltose reduces transfusions and hospital stay in patients with colon cancer and anemia (2015)

Preoperative ferric carboxymaltose treatment in patients with CC and iron deficiency anemia significantly reduced RBC transfusion requirements and hospital length of stay, reaching higher response rates and percentages of normalized hemoglobin levels both at hospital admission and 30 days post-surgery.
A Novel Targeting Drug Delivery System Based on Self-Assembled Peptide Hydrogel (2011)

In the last two decades, 5-fluorouracil (5-FU) is widely used in clinical practice to inhibit the fibroblasts to proliferate and improve the success rate of glaucoma-filtering surgery, but 5-FU has many toxic effects to normal ocular tissues. The self-assembled peptide hydrogels may serve as a new class of biomaterials for applications including tissue engineering and drug delivery. How to deliver 5-FU quickly and precisely to the target sites of ocular tissue by a self-assembled peptide hydrogel remains unexplored. RGD (arginine-glycine-aspartic acid) sequence is cell attachment site in extracellular matrix (ECM). Thus, If the self-assembled peptide hydrogel containing the RGD sequence that act as a specific attachment site for the proliferated fibroblasts adhesion could be designed, after integrated 5-FU, a novel targeting drug delivery system will be put into practice in the future.
A Group II Intron-Type Open Reading Frame from the Thermophile Bacillus ( Geobacillus ) stearothermophilus Encodes a Heat-Stable Reverse Transcriptase (2004)

The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the synthesis of full-length cDNAs is frequently inefficient, because the RT commonly used often produces truncated cDNAs. Synthesizing cDNA at higher temperatures, on the other hand, can provide a number of improvements. These include increasing the length of cDNA product, greater accuracy, and greater specificity during reverse transcription. Thus, an RT that remains stable and active at hot temperatures may produce better-quality cDNAs and improve the yield of full-length cDNAs. Described here is the discovery of a gene, designated trt, from the genome of the thermophilic bacterium Bacillus (Geobacillus) stearothermophilus strain 10. The gene codes for an open reading frame (ORF) similar to the ORFs encoded by group II introns found in bacteria. The gene was cloned and overexpressed in Escherichia coli, and its protein product was partially purified. Like the host organism, the Trt protein is a heat-stable protein with RT activity and can reverse transcribe RNA at temperatures as high as 75 degrees C.
PERP gene therapy attenuates lung cancer xenograft via inducing apoptosis and suppressing VEGF (2011)

Inducing apoptosis is an attractive antitumor strategy. PERP is an apoptosis-associated target of p53, and its activation alone is sufficient to induce apoptotic pathway leading to cell death. We have previously demonstrated that overexpression of PERP in tumor cell lines with low intrinsic PERP activity suppressed cancer cell growth and enhanced sensitivity to chemotherapeutical agents. We further identified that PERP was present in surgical normal lung tissue, but absent in cancerous tissue of the same patient. Here, we sought to investigate the anti-tumor effects of PERP gene therapy in vivo. Then nude mice were transplanted with p53-mutanted Anip973 human lung cancer xenografts and treated with normal saline, pcDNA3.1 (vector) and pcDNA3.1-PERP, respectively. Successful transfection and robust expression of PERP was detected. Treatment with pcDNA3.1-PERP increased apoptosis and retarded growth in the xenografts, which contributed to a 55% decrease in tumor volume compared with controls. Furthermore, PERP gene therapy activated pro-apoptotic Caspase-3 cascade and upregulated the expression of the second mitochondria-derived activator of caspase (Smac) and human TNF-related apoptosis-inducing ligand (TRAIL), while suppressed vascular endothelial growth factor (VEGF) expression, indicating apoptosis and anti-angiogenesis are involved in the inhibitory effect of the PERP gene therapy. Taken together, our results suggest PERP gene therapy may supply an alternative strategy for lung adenocarcinoma management. Furthermore, Anip973 is a p53-mutanted cell line and the findings of this study provide reference value for other p53-mutanted cancers which is common among malignant tumors.
Prognosis with neural networks using statistically based feature sets (2003)

The authors report on several techniques for feature selection utilized in the development of a prognostic tool of predicting recovery for patients with head trauma injuries. The database was examined for features, which were extracted using statistical techniques. ANN (artificial neural network) models were built based on the feature selection of the statistical techniques. These models were trained and tested. Results showed that the ability of the ANN to generalize was dependent on three factors: method of data representation, number of outcome classes, and specific features in the data set. The ANN architecture was kept constant for all the cases. Of the statistical techniques used, the backward selection applied to RA (regression analysis) and stepwise selection applied to LDA (linear disciminant analysis) feature models yielded the best generalizations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Microfiberoptic evaluation of the middle ear cavity. (1994)

Endoscopic instruments have revolutionized surgical diagnosis and treatment. Recently, a high resolution microfiberoptic endoscope has been developed that has vast potential for otologic use. This microfiberoptic endoscope was used in cadaver and human studies to visualize the middle ear cavity. The technique used involved placing a 1.0-mm or smaller microfiberoptic scope into the middle ear via a tympanic membrane perforation, through a myringotomy tube or up the eustachian tube. Using the scope, the mesotympanum and hypotympanum can be well visualized. Similarly, the round window, oval window, ossicular chain, and related structures can be clearly demonstrated and recorded photographically. This technique has great potential to enhance diagnosis without open surgery.
Predicting Alzheimer’s Disease Using Combined Imaging-Whole Genome SNP Data (2015)

The growing public threat of Alzheimer's disease (AD) has raised the urgency to discover and validate prognostic biomarkers in order to predicting time to onset of AD. It is anticipated that both whole genome single nucleotide polymorphism (SNP) data and high dimensional whole brain imaging data offer predictive values to identify subjects at risk for progressing to AD. The aim of this paper is to test whether both whole genome SNP data and whole brain imaging data offer predictive values to identify subjects at risk for progressing to AD. In 343 subjects with mild cognitive impairment (MCI) enrolled in the Alzheimer's Disease Neuroimaging Initiative (ADNI-1), we extracted high dimensional MR imaging (volumetric data on 93 brain regions plus a surface fluid registration based hippocampal subregion and surface data), and whole genome data (504,095 SNPs from GWAS), as well as routine neurocognitive and clinical data at baseline. MCI patients were then followed over 48 months, with 150 participants progressing to AD. Combining information from whole brain MR imaging and whole genome data was substantially superior to the standard model for predicting time to onset of AD in a 48-month national study of subjects at risk. Our findings demonstrate the promise of combined imaging-whole genome prognostic markers in people with mild memory impairment.
Antineoplastic cyclic peptides from the marine tunicate Lissoclinum patella (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAntineoplastic cyclic peptides from the marine tunicate Lissoclinum patellaChris M. Ireland, Augustine R. Durso Jr., Robert A. Newman, and Miles P. HackerCite this: J. Org. Chem. 1982, 47, 10, 1807–1811Publication Date (Print):May 1, 1982Publication History Published online1 May 2002Published inissue 1 May 1982https://pubs.acs.org/doi/10.1021/jo00349a002https://doi.org/10.1021/jo00349a002research-articleACS PublicationsRequest reuse permissionsArticle Views571Altmetric-Citations157LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Effects of Activators on Zirconium Phosphinimide Ethylene Polymerization Catalysts (2004)

Synthetic routes to the species CpZr(NPt-Bu3)2Cl, 7, Cp2Zr(NPt-Bu3)Cl, 8, CpZr(NPt-Bu3)2Me, 9, Cp2Zr(NPt-Bu3)Me, 10, and CpZr(NPt-Bu3)2Bn, 11, were developed in a manner similar to that previously reported for zirconium phosphinimide complexes. Rather than employing metathesis routes, transamination was considered to synthesize bis-phosphinimide zirconium complexes. At ambient temperature, Zr(NPt-Bu3)3(NMe2), 15, was isolated in less than 5% yield, but could be obtained cleanly via reaction of Zr(NPt-Bu3)3Cl, 14, with LiNMe2. However, thermolysis of Zr(NEt2)4 with HNPt-Bu3 afforded Zr(NPt-Bu3)2(NEt2)2, 12, which was subsequently converted to Zr(NPt-Bu3)2Cl2, 13, upon reaction with trimethylsilyl chloride. Cationic products were generated from the reaction of Lewis acids in the presence of a donor to provide the salts [CpZr(NPt-Bu3)Me(THF)][MeB(C6F5)3], 16, [Cp*Zr(NPt-Bu3)((i-PrN)2CMe)][MeB(C6F5)3], 17, and [CpZr(NPt-Bu3)((i-PrN)2CMe)][MeB(C6F5)3], 18. Similarly, reaction of [HNMe2Ph][B(C6F5)4] with 4 generated the salt [CpZr(NPt-Bu3)Me(NMe2Ph)][B(C6F5)4], 19, while reaction of 11 with B(C6F5)3 gave the base-free product [CpZr(NPt-Bu3)2][BnB(C6F5)3], 20. Structural considerations and preliminary MO calculations support the reactivity studies that augur well for olefin polymerization activity. Experimentally, previously reported screening using MAO as a solvent scrubber/activator with 1−4 showed only moderate polymerization activities. However, use of 20 equiv of Al(i-Bu)3 as scavenger and 2 equiv of B(C6F5)3 as cocatalyst resulted in a significant increase in activity relative to that observed upon activation with MAO. Use of [Ph3C][B(C6F5)4] as the cocatalyst led to even higher ethylene polymerization activities.
Genetic background determines the requirement for B7 costimulation in induction of autoimmunity (2002)

B7 costimulatory molecules play an important role in inducing autoimmunity, tumor immunity, and transplant rejection, and therapeutic manipulation of B7 is being investigated in human diseases. To determine whether B7 costimulation is essential for inducing autoimmunity on different genetic backgrounds, we backcrossed B7.1/B7.2 deficient ((-/-)) mice on to the C57BL/6 (B6) and SJL backgrounds and induced experimental autoimmune encephalomyelitis (EAE) in these mice. B7.1/B7.2(-/-) mice on the B6 background were resistant to EAE induced with MOG 35-55, whereas the SJL B7.1/B7.2(-/-) mice were susceptible to PLP 139-151 or PLP 178-191-induced EAE. The SJL B7.1/B7.2(-/-) mice had a qualitatively different lesion pattern in that they showed increased white matter vacuolation compared to wild-type SJL mice when immunized with either PLP 139-151 or PLP 178-191. (B6xSJL)F1 B7.1/B7.2(+/+) mice were susceptible to EAE whereas (B6xSJL)F1 B7.1/B7.2(-/-) mice were resistant to EAE induced with either encephalitogenic peptide. Thus, genetic background determines the B7 requirement for inducing autoimmunity. These data have important implications for developing B7-based immunotherapies for human diseases.
Thiazide Diuretics in the Treatment of Hypertension (2006)

Thiazide diuretics were the first tolerated efficient antihypertensive drugs that significantly reduced cardiovascular morbidity and mortality in placebo-controlled clinical studies. Although these drugs today still are considered a fundamental therapeutic tool for the treatment of hypertensive patients, the following considerations should be taken into account. Although there are some indications that chlorthalidone can offer additional advantages as compared with other compounds, a recent meta-analysis of placebo-controlled trials suggested that the beneficial effects of thiazide diuretics could be a class effect. Thiazide diuretics must be used at appropriate and/or optimal doses to achieve the optimal antihypertensive effect with the smallest occurrence of side effects, including alterations in glucose and lipid profiles and hypokalemia. Moreover, because thiazide diuretics can increase the incidence of new-onset diabetes, especially when combined with beta blockers, caution is advised in using these drugs above all in patients who are at high risk for developing diabetes, in whom thiazide diuretics should be used at the lowest active dose and possibly in combination with drugs that block the renin-angiotensin system. Finally, the current debate on whether thiazide diuretics are the first-choice drug for most patients with uncomplicated hypertension, as stated in the Seventh Joint National Committee Report, or are included in the major classes of antihypertensive agents that are suitable for initiation and maintenance of therapy, as reported in the European Society of Hypertension-European Society of Cardiology Guidelines, derives from different interpretations of controlled clinical trial data on drug class comparison and of cost-benefit analyses. However, considering that the benefit of antihypertensive drugs seems to be due principally to BP lowering per se without definitive evidence of the superiority of a particular drug class and that there is no cost-benefit analysis showing the superiority of thiazide diuretics, it is believed that these drugs should not be considered as the only first-choice drug but included among first-choice drugs.
NEURONAL Ca2+/CALMODULIN-DEPENDENT PROTEIN KINASES (1992)

Widespread localization, responsiveness to numerous signal transduction systems, and broad substrate specificity enable the multifunctional CaM kinase to mediate regulation of many cellular functions. The abundance and diversity of CaM kinase substrates attest to its role as a multifunctional kinase. However, expanded identification of its in situ substrates as well as the consequences of their regulation by phosphorylation needs to be accomplished. Recently identified substrates have contributed to the list of potential functions for the CaM kinase. CREB is a hormonally stimulated transcriptional activator, and CaM kinase may lie on the pathway to its activation. This pathway could provide an interface between the potentiation of Ca2+ signals by CaM kinase and longer-term modifications of neuronal gene expression. The ryanodine receptor, as well as phospholamban, are involved in cardiac Ca2+ homeostasis, and their regulation by CaM kinase phosphorylation suggests the possibility of some feedback control of intracellular Ca2+ levels by CaM kinase. Regulation of neuronal plasticity by phosphorylation of synapsin I and of postsynaptic substrates necessary for long-term potentiation is another dynamic area of investigation. The study of substrates and their functions promises to continue providing exciting insights into the control of cellular signalling by Ca2+. Molecular cloning has enabled structural comparison of neuronal isoforms of the kinase, and has revealed the existence of closely related subunits. Subunits identified to data differ substantially only in two small variable domains, yet their expression in various tissues and during the course of development is precisely controlled. What unique properties do these small variable domains impart to the different isoforms? What directs high concentrations of kinase to a particular subcellular localization, and especially to the PSD? Further molecular cloning will undoubtedly determine whether other multifunctional CaM kinases with unique structures and properties exist. Finally, studies on the autoregulatory properties of CaM kinase have provided a fascinating picture of how this molecule can alone encode responses to Ca2+ signals, potentiating both the duration and magnitude of its activity. Autophosphorylation of the Thr286 autonomy site both traps calmodulin and permits Ca(2+)-independent activity after calmodulin dissociates. Further analysis of the role of the holoenzyme structure in these modulations will help clarify remaining mechanistic questions. Studies performed during the past few years have clearly established that this Ca(2+)-independent activity is generated in situ in response to a variety of cell stimuli.(ABSTRACT TRUNCATED AT 400 WORDS)
Hereditary Protein S Deficiency in Young Adults with Arterial Occlusive Disease (1990)

Protein S is the vitamin K dependent cofactor of activated protein C. It has an important role in the regulation of blood coagulation and fibrinolysis. Hereditary protein S deficiency is associated with familial venous thrombophilia. Since a few patients with arterial occlusions have been reported to be protein S deficient, it is speculated that hereditary protein S deficiency may be also a risk factor for the development of arterial thrombosis. In a group of 37 consecutive patients with arterial occlusive disease presenting before the age of 45, three patients were found heterozygous for hereditary protein S deficiency. None of the patients had a protein C deficiency or an antithrombin III deficiency. Family investigations showed a clear association between the hereditary deficiency and venous thrombosis, but a relation between the deficiency and arterial thrombosis was less obvious. A review of previous literature on patients with arterial thrombosis and protein S deficiency revealed that more extensive studies are needed to demonstrate whether or not hereditary protein S deficiency is a risk factor for the development of arterial thrombosis.
Towards the development of latent heat storage electrodes for electroporation-based therapies (2012)

Phase change materials (PCMs) capable of storing a large amount of heat upon transitioning from the solid-to-liquid state have been widely used in the electronics and construction industries for mitigating temperature development. Here, we show that they are also beneficial for reducing the peak tissue temperature during electroporation-based therapies. A numerical model is developed of irreversible electroporation (IRE) performed with hollow needle electrodes filled with a PCM. Results indicate that this electrode design can be utilized to achieve large ablation volumes while reducing the probability for thermal damage.
Device study, chemical doping, and logic circuits based on transferred aligned single-walled carbon nanotubes (2008)

In this paper, high-performance back-gated carbon nanotube field-effect transistors based on transferred aligned carbon nanotubes were fabricated and studies found that the on/off ratio can reach 107 and the current density can reach 1.6μA∕μm after electrical breakdown. In addition, chemical doping with hydrazine was used to convert the p-type aligned nanotube devices into n-type. These devices were further utilized to demonstrate various logic circuits, including p-type metal-oxide-semiconductor inverters, diode-loaded inverters, complementary metal-oxide-semiconductor inverters, NAND, and NOR gates. This approach could work as the platform for future nanotube-based nanoelectronics.
Variability in pain expression characteristics in former preterm infants (2005)

Correlations of PCA with pre-procedural and relative increase in heart rate reflect the maturational decrease in heart rate. LOS had an effect on pain expression, potentially reflecting the impact of cumulative 'minor' procedural pain.
Modulation of human dendritic cell phenotype and function by probiotic bacteria (2004)

Probiotic bacteria differ in their immunomodulatory activity and influence polarisation of immune responses at the earliest stage of antigen presentation by dendritic cells.
A Morphogenetically Competent Soybean Suspension Culture (1983)

A morphogenetically competent suspension culture was derived from embryonic axes of Glycine max cv. Mitchell. The cultural history included visual selection for nonfriable, embryo-like structures, recurrent selection in a regime of 2,4-dichlorophenoxyacetic acid exposure and withdrawal, and the replacement of the nitrogen in a Murashige and Skoog salts-based medium with 20 millimolar ammonium citrate. The embryoids produced by this suspension are capable of completing plantlet development. The suspension can be maintained by serial subculture.