gebhart/patrepeval huggingface dataset:
Shedding of the Type II IL-1 Decoy Receptor Requires a Multifunctional Aminopeptidase, Aminopeptidase Regulator of TNF Receptor Type 1 Shedding (2003)

Proteolytic cleavage of the extracellular domain of the type II IL-1 decoy receptor (IL-1RII) generates soluble IL-1-binding proteins that prevent excessive bioactivity by binding free IL-1. In this study we report that an aminopeptidase, aminopeptidase regulator of TNFR1 shedding (ARTS-1), is required for IL-1RII shedding. Coimmunoprecipitation experiments demonstrate an association between endogenous membrane-associated ARTS-1 and a 47-kDa IL-1RII, consistent with ectodomain cleavage of the membrane-bound receptor. A direct correlation exists between ARTS-1 protein expression and IL-1RII shedding, as cell lines overexpressing ARTS-1 have increased IL-1RII shedding and decreased membrane-associated IL-1RII. Basal IL-1RII shedding is absent from ARTS-1 knockout cell lines, demonstrating that ARTS-1 is required for constitutive IL-1RII shedding. Similarly, PMA-mediated IL-1RII shedding is almost entirely ARTS-1-dependent. ARTS-1 expression also enhances ionomycin-induced IL-1RII shedding. ARTS-1 did not alter levels of membrane-associated IL-1RI or IL-1R antagonist release from ARTS-1 cell lines, which suggests that the ability of ARTS-1 to promote shedding of IL-1R family members may be specific for IL-1RII. Further, increased IL-1RII shedding by ARTS-1-overexpressing cells attenuates the biological activity of IL-1beta. We conclude that the ability of ARTS-1 to enhance IL-1RII shedding represents a new mechanism by which IL-1-induced cellular events can be modulated. As ARTS-1 also promotes the shedding of the structurally unrelated 55-kDa, type I TNF receptor and the IL-6R, we propose that ARTS-1 may play an important role in regulating innate immune and inflammatory responses by increasing cytokine receptor shedding.
Stereoselective aldol reactions of chlorotitanium enolates. An efficient method for the assemblage of polypropionate-related synthons (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereoselective aldol reactions of chlorotitanium enolates. An efficient method for the assemblage of polypropionate-related synthonsDavid A. Evans, Dale L. Rieger, Mark T. Bilodeau, and Felix UrpiCite this: J. Am. Chem. Soc. 1991, 113, 3, 1047–1049Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January 1991https://pubs.acs.org/doi/10.1021/ja00003a051https://doi.org/10.1021/ja00003a051research-articleACS PublicationsRequest reuse permissionsArticle Views4000Altmetric-Citations288LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. (1996)

A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs within the target population and the subtraction step excludes the common sequences between the target and driver populations. In a model system, the SSH technique enriched for rare sequences over 1,000-fold in one round of subtractive hybridization. We demonstrate its usefulness by generating a testis-specific cDNA library and by using the subtracted cDNA mixture as a hybridization probe to identify homologous sequences in a human Y chromosome cosmid library. The human DNA inserts in the isolated cosmids were further confirmed to be expressed in a testis-specific manner. These results suggest that the SSH technique is applicable to many molecular genetic and positional cloning studies for the identification of disease, developmental, tissue-specific, or other differentially expressed genes.
Biochemical Analysis of Angelman Syndrome-associated Mutations in the E3 Ubiquitin Ligase E6-associated Protein (2004)

Angelman syndrome is a severe neurological disorder characterized by mental retardation, absent speech, ataxia, seizures, and hyperactivity. The gene affected in this disorder is UBE3A, the gene encoding the E6-associated protein (E6AP) ubiquitin-protein ligase. Most patients have chromosomal deletions that remove the entire maternal allele of UBE3A. However, a small subset of patients have E6AP point mutations that result in single amino acid changes or short in-frame deletions that still allow translation of a full-length protein. By studying these point mutations in E6AP, we found a strong correlation between Angelman-associated mutations and a loss of E3 ubiquitin ligase activity. Interestingly the point mutations affect E6AP activity in different ways. Some mutant proteins cannot form thiol ester intermediates with ubiquitin, others retain the thiol ester formation activity but cannot efficiently transfer ubiquitin to a substrate, and still others are unstable in cells. Our results suggest that the loss of E6AP catalytic activity and likely the improper regulation of E6AP substrate(s) are important in the development of Angelman syndrome.
Radial Keratotomy: Preliminary Report of Complications (1982)

We have observed the following complications with radial keratotomy which require further evaluation: missing the visual axis while marking the central cornea, regression of myopic flattening, epithelial defects, recurrent erosions, stromal overgrowth, Cogan's map-dot fingerprint corneal dystrophy with Moncreiff iron lines, blood in the incisions, vascular ingrowth, perforation of the anterior chamber, induction of astigmatic errors, epithelial ingrowth, glare complaints and decreased night vision, pain, fluctuating vision from morning to evening, overcorrection and unpredictable results, contact lens refitting difficulties, endothelial cell loss, and corneal scarring. We feel these complications require extensive further study before the relative safety and long-term efficacy of this experimental procedure may be determined.
Speech enhancement and coding in harsh acoustic noise environments (2003)

Recent advances in speech enhancement and noise pre-processing algorithms have dramatically improved the quality and intelligibility of speech signals, both in the presence of acoustic noise as well as in benign environments. The use of speech enhancement in combination with voice coding algorithms and applied to governmental wireless communications systems is an important application area. This paper will first introduce one such system, the 2.4 kbps US Government Military Standard mixed excitation linear prediction (MELP) speech coding algorithm coupled with a speech enhancement algorithm developed by AT&T Research Labs. Next, the paper presents a discussion of the test conditions and results, and provide an interpretation of these results. Finally, a general discussion of related issues and conclusions is presented.
Tumor-Infiltrating Dendritic Cells in Cancer Pathogenesis (2015)

Dendritic cells (DCs) play a pivotal role in the tumor microenvironment, which is known to affect disease progression in many human malignancies. Infiltration by mature, active DCs into the tumors confers an increase in immune activation and recruitment of disease-fighting immune effector cells and pathways. DCs are the preferential target of infiltrating T cells. However, tumor cells have means of suppressing DC function or of altering the tumor microenvironment in such a way that immune-suppressive DCs are recruited. Advances in understanding these changes have led to promising developments in cancer-therapeutic strategies targeting tumor-infiltrating DCs to subdue their immunosuppressive functions and enhance their immune-stimulatory capacity.
Low-temperature thermal oxide to plasma-enhanced chemical vapor deposition oxide wafer bonding for thin-film transfer application (2003)

Low-temperature direct plasma-enhanced chemical vapor deposition (PECVD) oxide to thermal oxide bonding is described. The PECVD oxide is densified at 350 °C and chemical-mechanically polished to obtain reasonably smooth surface for bonding. The PECVD oxide wafer is bonded to the thermal oxide wafer at room temperature after piranha clean that leaves the wafer surfaces hydrophilic. A postbonding anneal at 300 °C completes the bonding. A void-free bonding interface is observed from infrared imaging and the bonding strength is estimated to be 432 mJ/m2. This bonding method can be used in a variety of applications, including three-dimensional integration.
Peptides as receptor ligand drugs and their relationship to G-coupled signal transduction (2001)

Peptides act as effector agents that regulate and/or mediate physiological processes, serving as hormones, neurotransmitters and signal transducing factors. The low molecular weight peptides affect receptor-mediated events, which influence cardiovascular, gastrointestinal and neurocranial systems. While some peptides have been marketed as drugs, many have served as leads or templates for the development of non-peptide drugs that mimic peptide actions. This review presents the advantages and disadvantages of using peptides as drugs that bind as ligands to cell-surface receptors and considers their applications in such events. The value of both the peptides and their mimics is based on their participation in the biomodulation of physiological processes, which frequently employ scaffolding proteins acting in a cascading sequence of protein-to-protein interactions. The peptides bind to G-coupled surface receptors to initiate a signal that is transduced to the interior of the cell through multiple layers of phosphorylating enzymes and binding proteins. Peptides have been further employed to identify the molecular targets of signal transduction, the uncoupling of which might provide a means for various disease therapies. The exploitation of such peptide-mediated signal pathways, which are of primary importance to tumour cells, may provide an attractive strategy for anticancer therapy in the future.
Speciation Analysis for Iodine in Groundwater Using High Performance Liquid Chromatography‐Inductively Coupled Plasma‐Mass Spectrometry (HPLC‐ICP‐MS) (2007)

Methods based on high performance liquid chromatography coupled with inductively coupled plasma‐mass spectrometry (HPLC‐ICP‐MS) for iodine speciation analysis are described. Experiments were performed relating to iodine species, preservative medium and time. The concentration and pH of the buffer are also discussed in relation to separation efficiency. The developed methods allowed the fast and sensitive determination of iodine species with detection limits of 0.025 μg l −1 (as 1) for both iodide and iodate with &lt; 5% relative standard deviation (RSD) for 50 nmol l −1 . Attempts were made to quantify total organo‐iodine by size‐exclusion chromatography (SEC). By way of example, a number of groundwater samples were analysed by these methods, revealing that iodide is the main iodine species in the sampled waters, but that high concentrations of organo‐iodine compounds were observed in some samples as well. In summary, the total concentration and the dominant species of iodine in aquatic freshwater environments is easily and accurately measured using this new method.
Combined Source and Channel Coding for Variable-Bit-Rate Speech Transmission (1983)

Motivated by potential applications to mobile radio, we studied variable-bit-rate speech communication through Gaussian-noise and Rayleigh-fading channels. For convenience we used a constant signaling rate of 32 kb/s and adjusted the source-coding and channel-coding rates in response to changing transmission quality. When the channel quality was good enough, we used all 32 kb/s for speech transmission. When the channel quality was lower, we reduced the source rate to 24 or 16 kb/s and introduced channel coding to control distortion due to transmission errors. We concentrated on specific source and channel codes that could be implemented with hardware of modest complexity. The source code was embedded differential pulse code modulation, which is amenable to variable-bit-rate operation and economical to implement. For error control we introduced punctured convolutional codes and a Viterbi decoder with only 16 states. Although the source/channel codec was simple, it offered good performance. Speech quality was at the level of normal telephony when the channel was good; the error-correcting codes extended by up to 13 dB the range of channel signal-to-noise ratios that support adequate quality. Our performance estimates were based on a new analysis of transmission errors in embedded differential pulse code modulation and on computer simulations of speech transmission through fixed and fading channels.
Architecture of the Human Ndc80-Hec1 Complex, a Critical Constituent of the Outer Kinetochore (2005)

The Ndc80 complex is a constituent of the outer plate of the kinetochore and plays a critical role in establishing the stable kinetochore-microtubule interactions required for chromosome segregation in mitosis. The Ndc80 complex is evolutionarily conserved and contains the four subunits Spc24, Spc25, Nuf2, and Ndc80 (whose human homologue is called Hec1). All four subunits are predicted to contain globular domains and extensive coiled coil regions. To gain an insight into the organization of the human Ndc80 complex, we reconstituted it using recombinant methods. The hydrodynamic properties of the recombinant Ndc80 complex are identical to those of the endogenous HeLa cell complex and are consistent with a 1:1:1:1 stoichiometry of the four subunits and a very elongated shape. Two tight Hec1-Nuf2 and Spc24-Spc25 subcomplexes, each stabilized by a parallel heterodimeric coiled coil, maintain this organization. These subcomplexes tetramerize via an interaction of the C- and N-terminal portions of the Hec1-Nuf2 and Spc24-Spc25 coiled coils, respectively. The recombinant complex displays normal kinetochore localization upon injection in HeLa cells and is therefore a faithful copy of the endogenous Ndc80 complex.
Biotype‐specific expression of dsRNA in the sweetpotato whitefly (1994)

Abstract This study was conducted to evaluate the effect of two different biotypes of the sweetpotato whitefly, Bemisia tabaci (Gennadius), on the induction of squash silverleaf (SSL), and to determine if double‐stranded RNA (dsRNA) occurs in geographically remote populations of the two biotypes. Recently collected B‐biotype whiteflies from Florida, Arizona, Mississippi, and Texas (SPW‐B) all contained a 7.0 kb dsRNA molecule. Kb dsRNA molecule. Laboratory colonies of A‐biotype whiteflies that were originally collected in 1981 from cotton in Arizona and California did not contain the 7.0 Kb dsRNA. When the two biotypes were compared only the SPW‐B induced rapid onset, grade 5, SSL. DsRNA similar to that found in adult SPW‐B was concentrated in whitefly nymphs, but host plant leaf tissue did not contain any consistent dsRNA molecules. SPW‐A only induced low‐grade SSL and progeny of SPW‐A that were fed on pumpkin plants displaying SSL did not acquire the ability to express dsRNA or induce SSL. Our data suggest that dsRNA is not directly involved in the induction of SSL and that SSL is a host‐specific response to a feeding injury induced by B‐biotype whiteflies. The origin and source of the 7.0 Kb dsRNA molecule remains enigmatic but its expression is constant in the whitefly biotype that is responsible for the induction of SSL and several other plant disorders in the U.S.
Seeing through the Human Reporting Bias: Visual Classifiers from Noisy Human-Centric Labels (2016)

When human annotators are given a choice about what to label in an image, they apply their own subjective judgments on what to ignore and what to mention. We refer to these noisy "human-centric" annotations as exhibiting human reporting bias. Examples of such annotations include image tags and keywords found on photo sharing sites, or in datasets containing image captions. In this paper, we use these noisy annotations for learning visually correct image classifiers. Such annotations do not use consistent vocabulary, and miss a significant amount of the information present in an image, however, we demonstrate that the noise in these annotations exhibits structure and can be modeled. We propose an algorithm to decouple the human reporting bias from the correct visually grounded labels. Our results are highly interpretable for reporting "what's in the image" versus "what's worth saying." We demonstrate the algorithm's efficacy along a variety of metrics and datasets, including MS COCO and Yahoo Flickr 100M.We show significant improvements over traditional algorithms for both image classification and image captioning, doubling the performance of existing methods in some cases.
附：鲁迅的《野草》 (2010)

《野草》是鲁迅先生唯一的散文集子。这集子里的文章是他在一九二四到一九二六年中写成的。鲁迅先生的作品，以杂文最多，小说、译作次之，唯散文则仅此一集；但他散文的精美坚实，在中国文学史上，实无人足以比拟。这集子里所收的每一篇，都可以说是最真实的诗篇，是作者从当时个人生活所遭受的惨痛和激动中所直接抒发的思想情感的结晶。在这里，我们所感到的是种热辣辣的火与剑的情感，一个单枪匹马在重重黑暗包围中坚韧不屈地战斗着的战士底情感。它呈现在我们面前的，“有深奥或陡峻的境界，特殊地美而且根底上也是善的，有惨美的病态的情绪和意境，根底上是善的，
PROMALS: towards accurate multiple sequence alignments of distantly related proteins (2007)

Supplementary data are available at Bioinformatics online.
A Synthetic Factor VIII Peptide of Eight Amino Acid Residues (1677-1684) Contains the Binding Region of an Anti-Factor VIII Antibody which Inhibits the Binding of Factor VIII to von Willebrand Factor (1990)

The monoclonal anti-factor VIII (FVIII) antibody C4 has previously been reported to inhibit the binding of purified FVIII to immobilized von Willebrand factor (vWF). The binding area of C4 was identified to be within fifteen amino acid residues (1670-1684) based on the ability of a synthetic FVIII peptide consisting of amino acid residues 1670-1684 to completely inhibit the binding of C4 to FVIII. We now report the further localization of the binding region of C4 to within eight amino acid residues (1677-1684) of FVIII light chain. Nine new overlapping FVIII peptides were synthesized based on the amino acid sequence of the acidic region of FVIII light chain and tested, along with seven previously tested peptides, for the ability to inhibit C4 binding to FVIII in an ELISA assay. Three synthetic FVIII peptides 1670-1684, 1675-1690, and 1677-1684 demonstrated dose dependent inhibition of C4 binding to FVIII. The three reactive peptides contain residues 1677-1684 in common. Since C4 can completely inhibit the binding of FVIII to vWF, this report further localizes an eight amino acid residue region of FVIII which may be important in the mediation of vWF binding.
Blockade of Epidermal Growth Factor- or Heregulin-Dependent ErbB2 Activation with the Anti-ErbB2 Monoclonal Antibody 2C4 Has Divergent Downstream Signaling and Growth Effects (2004)

Due to heterodimerization and a variety of stimulating ligands, the ErbB receptor system is both diverse and flexible, which proves particularly advantageous to the aberrant signaling of cancer cells. However, specific mechanisms of how a particular receptor contributes to generating the flexibility that leads to aberrant growth regulation have not been well described. We compared the utilization of ErbB2 in response to epidermal growth factor (EGF) and heregulin stimulation in colon carcinoma cells. Anti-ErbB2 monoclonal antibody 2C4 blocked heregulin-stimulated phosphorylation of ErbB2 and ErbB3; activation of mitogen-activated protein kinase (MAPK), phosphatidylinositol 3'-kinase (PI3K), and Akt; proliferation; and anchorage-independent growth. 2C4 blocked EGF-mediated phosphorylation of ErbB2 and inhibited PI3K/Akt and anchorage-independent growth but did not affect ErbB1 or MAPK. Immunoprecipitations showed that ErbB3 and Grb2-associated binder (Gab) 1 were phosphorylated and associated with PI3K activity after heregulin treatment and that Gab1 and Gab2, but not ErbB3, were phosphorylated and associated with PI3K activity after EGF treatment. These data show that monoclonal antibody 2C4 inhibited all aspects of heregulin signaling as well as anchorage-independent and monolayer growth. Furthermore, we identify ErbB2 as a critical component of EGF signaling to the Gab1/Gab2-PI3K-Akt pathway and anchorage-independent growth, but EGF stimulation of MAPK and monolayer growth can occur efficiently without the contribution of ErbB2.
p-Nitroaniline/glycerol: a binary liquid matrix for MALDI analysis (1998)

Liquid matrices for matrix-assisted laser desorption/ionization (MALDI) analysis offer advantages over solid matrices including increased shot-to-shot reproducibility and the ability to provide conditions more suitable for the retention of non-covalent complexes. Unfortunately, most of the liquid matrices available do not absorb at UV wavelengths. A second component to increase chromophoric absorption and to permit control over the chemical properties of the matrix can aid in overcoming some of the limitations currently faced by the MALDI technique. In the work reported here, a binary matrix that combines the advantages of the fluidity and low vapor pressure of glycerol with the weak basicity and chromophoric properties of p-nitroaniline is evaluated. Both positive-ion and negative-ion spectra are obtained for proteins, carbohydrates and oligonucleotides. The potential for analysis of chelates and non-covalent interactions using this liquid binary matrix is also explored.
Molecular cloning, characterization, and chromosomal localization of dapF, the Escherichia coli gene for diaminopimelate epimerase (1987)

The Escherichia coli dapF gene was isolated from a cosmid library as a result of screening for clones overproducing diaminopimelate epimerase. Insertional mutagenesis was performed on the cloned dapF gene with a mini-Mu transposon, leading to chloramphenicol resistance. One of these insertions was transferred onto the chromosome by a double-recombination event, allowing us to obtain a dapF mutant. This mutant accumulated large amounts of LL-diaminopimelate, confirming the blockage in the step catalyzed by the dapF product, but did not require meso-diaminopimelate for growth. The dapF gene was localized in the 85-min region of the E. coli chromosome between cya and uvrD.