gebhart/patrepeval huggingface dataset:
Synthesis of a nonionic polymer surfactant from cyclic imino ethers by the initiator method (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of a nonionic polymer surfactant from cyclic imino ethers by the initiator methodShiro Kobayashi, Susumu Iijima, Toshio Igarashi, and Takeo SaegusaCite this: Macromolecules 1987, 20, 8, 1729–1734Publication Date (Print):August 1, 1987Publication History Published online1 May 2002Published inissue 1 August 1987https://pubs.acs.org/doi/10.1021/ma00174a001https://doi.org/10.1021/ma00174a001research-articleACS PublicationsRequest reuse permissionsArticle Views405Altmetric-Citations57LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Reconstitution of the vitamin D-responsive osteocalcin transcription unit in Saccharomyces cerevisiae. (1989)

The human osteocalcin gene is regulated in mammalian osteoblasts by 1,25(OH)2D3-dependent and -independent mechanisms. The sequences responsible for this activity have been mapped to within the -1339 region of the gene. We show here that this enhancer region functions analogously in Saccharomyces cerevisiae cells engineered to produce active 1,25(OH)2D3 receptor. When fused to the proximal promoter elements of the yeast iso-1-cytochrome c gene, the enhancer demonstrated substantial promoter activity. This activity was elevated further by 1,25(OH)2D3 when the reporter constructs were assayed in cells containing the 1,25(OH)2D3 receptor. This system affords a model for 1,25(OH)2D3 action and represents a simple assay system that will enable definition of the important cis-acting regulatory sequences within the osteocalcin gene and identification of their cognate transcription factors.
Error burst metrics for failure trajectory analysis (1994)

The current performance monitoring strategies for International digital transmission systems are of a 'just too late' nature, whereby systems report as their performance crosses preset bounds which are deemed unacceptable. A new strategy increases network resilience and survivability, together with customer and operator satisfaction. This is achieved with a performance monitoring system based on predicting failure, postulating the cause and thereby allowing planned preventative maintenance.
Serum concentrations of acute phase proteins in dogs with leishmaniasis (2002)

The concentrations of haptoglobin, C-reactive protein and ceruloplasmin were measured in symptomatic and asymptomatic dogs naturally infected by Leishmania infantum, and in healthy uninfected dogs to determine the potential value of these proteins for the diagnosis and prognosis of leishmaniasis. The concentrations of the acute phase proteins were significantly higher in the dogs with leishmaniasis than in the control dogs, and the concentration of C-reactive protein was significantly higher in the symptomatic dogs than in the asymptomatic dogs. There were no correlations between the acute phase proteins and the gamma globulins, the albumin/globulin ratio or the titre of anti-leishmanial antibodies.
Controlled‐release of IGF‐I and TGF‐β1 in a photopolymerizing hydrogel for cartilage tissue engineering (2001)

Photopolymerizing hydrogel systems provide a method to encapsulate cells and implant materials in a minimally invasive manner. Controlled release of growth factors in the hydrogels may enhance the ability to engineer tissues. IGF-I and TGF-beta1 were loaded in PLGA microspheres using a double emulsion technique. 125 ng and 200 pg of active IGF-I and TGF-beta, respectively, as measured by ELISA, were released over 15 days. The growth factor containing microspheres were photoencapsulated with bovine articular chondrocytes in PEO-based hydrogels and incubated in vitro for two weeks. Statistically significant changes in glycosaminoglycan (GAG) production compared to control gels either without microspheres or with blank spheres were observed after a 14 day incubation with IGF-I and IGF-I/TGF-beta microspheres combined, with a maximum density of 8.41+/-2.5% wet weight GAG. Total collagen density was low and decreased with the IGF-I/TGF-beta microspheres after two weeks incubation, but otherwise remained unchanged in all other experimental groups. Cell content increased 10-fold to 0.18+/-0.056 x 10(6) cells/mg wet weight and extracellular matrix (ECM) staining by H&E increased in hydrogels with IGF-I/TGF-beta microspheres. In conclusion, photoencapsulation of microspheres in PEO-based hydrogels provides a method to deliver molecules such as growth factors in porous hydrogel systems.
Effect of the tripartite leader on synthesis of a non-viral protein in an adenovirus 5 recombinant (1985)

The EIa region of an Adenovirus 5 recombinant has been substituted by a modular gene encoding dihydrofolate reductase (DHFR). In this recombinant, the mouse DHFR cDNA was positioned behind sequences of the major late promoter and the complete tripartite leader. The leader sequences end in the normal 5' splice site (SS) of the third leader, so that RNA splicing joins the tripartite leader to a 3' splice site immediately upstream of the DHFR cDNA. At late stages of infection, high levels of DHFR mRNAs were synthesized. At early times in the late stage, this mRNA was efficiently translated; however, at later times translation of DHFR decreased probably due to poor competition with other late mRNAs. Synthesis of DHFR protein from an analogous Adenovirus 5 recombinant containing only the first late leader was studied in parallel. Equivalent levels of DHFR mRNA were expressed after infection with this recombinant virus; however, the efficiency of DHFR translation was at least 20 fold lower than that of the DHFR mRNA containing the tripartite leader. This suggests that the tripartite leader sequence is important for translation in the late stage of infection. As reported previously, the Ad5 recombinant containing only the first leader vastly overexpresses polypeptide IX from a novel mRNA, formed by the splicing of the first leader in the modular DHFR gene to the 3' splice site in the EIb region. Cells infected with this recombinant synthesize very little normal mRNA from the EIb region. Here, we demonstrated that coinfection of 293 cells with this recombinant and wild type Adenovirus 5 also results in decreased EIb mRNA synthesis. We propose that the overproduction of polypeptide IX suppresses mRNA expression from the EIb and IX promoter sites, probably by an autoregulation loop active during lytic growth.
Reaction between Ruthenium(0) Complexes and Dihalo Compounds. A New Method for the Synthesis of Ruthenium Olefin Metathesis Catalysts (1997)

The synthesis of ruthenium carbenes by the reaction of a Ru(0) compound or Ru(0) precursors with dihalo compounds is reported. A new procedure for the preparation of [Ru(H)2(H2)2(PCy3)2] and the use of this complex as a starting material for the synthesis of ruthenium carbenes are presented.
Pharmaceutical care - an overview (1996)

In discussing the concepts of pharmaceutical care there are a number of things to keep clearly in mind. Firstly, pharmaceutical care is patient specific i.e the patient rather than the drug is the focus of the service. Implicit in pharmaceutical care is the pharmacist taking responsibility for drug therapy decisions and outcomes. There is also a number of clear ethical principles involved in pharmaceutical care. (non-author abstract)
GM1 ganglioside therapy in acute ischemic stroke. (1990)

The statistically significant difference between GMl-treated and&#13;\nplacebo-treated patients that we found did not refer to the mean values of neurologic scores, which were similar among the groups, as clearly depicted in our Tables 4 and 5, but to the degree of neurologic improvement, as obtained by subtracting the Canadian Neurological Scale score at entry from the scores on days 1-15, 21, and 120, respectively. However, it is not clear what these differences in degree of neurologic improvement could mean in terms of functional recovery.&#13;\nWe completely agree with Hoffbrand and colleagues that the&#13;\nsuccess of a stroke treatment depends more on whether the&#13;\npatient is able to return home to useful function than on the activities of daily living scales are demanding and could not be&#13;\nused for stroke patients in the acute phase because performance is often limited by factors that are not dependent on their neurologic deficit. Furthermore, taking into account functional scales for long-term follow-up, we cannot know how much of their recovery is due to rehabilitation. In this context, it is interesting, rather than surprising, that we found a good correlation between mean neurologic global scores and the disability index, neither of which was statistically significant, confirming the functional value of the Canadian Neurological Scale score. Nevertheless, we think that no available parameters for evaluation of functional outcome in stroke patients, whether based on neurologic or disability scales, are sufficient. At present, one way to overcome this handicap could be to define more homogeneous and comparable subgroups of patients by stratifying them according to prognostic parameters such as age, severity of neurologic status at admission, and interval time between onset of symptoms and treatment. We think that only multicenter studies, capable of larger enrollment of a continuous series of patients, could help to reach this goal.&#13;\nOur study only partially fulfilled these criteria; for instance, the&#13;\ntime interval of 12 hours between stroke onset and treatment,&#13;\nalthough shorter than that of previous studies, was well over the socalled "therapeutic window." Moreover, our data are hardly comparable with those of previous studies due to significant differences in the study design, such as treatment not started in the acute phase, too small a sample size, and different outcome measures.&#13;\nWe therefore believe that the results we obtained need to be&#13;\nfurther tested in a larger population of 800 patients, stratified&#13;\naccording to sex, age, and Canadian Neurological Scale score at&#13;\nentry, and who are treated within 5 hours of stroke and for a longer period (21 days). Our Early Stroke Treatment trial will have the enrollment of the established number of patients, and we think that at the end of 1990 we will be able to provide our definitive results.
Triple-Reassortant Swine Influenza A (H1) in Humans in the United States, 2005–2009 (2009)

From December 2005 until just before the current human epidemic of swine-origin influenza viruses, there was sporadic infection with triple-reassortant swine influenza A (H1) viruses in persons with exposure to pigs in the United States. Although all the patients recovered, severe illness of the lower respiratory tract and unusual influenza signs such as diarrhea were observed in some patients, including those who had been previously healthy.
Triple-Negative Breast Cancer (2010)

Triple-negative breast cancer, so called because it lacks expression of the estrogen receptor, progesterone receptor, and HER2, is often, but not always, a basal-like breast cancer. This review focuses on its origin, molecular and clinical characteristics, and treatment.
Use of an Automated Method Improves the Yield and Quality of Cell-Free Fetal DNA Extracted from Maternal Plasma (2005)

Cell-free fetal DNA in the maternal circulation holds great potential for noninvasive prenatal diagnosis (1)(2). However, the manual isolation of cell-free DNA from plasma can be a time-consuming process, and current techniques involving spin columns usually require multiple reloadings of the columns with sample material, increasing the risk of contamination (3)(4)(5). Use of an automated system for DNA isolation can reduce the time involved, increase the throughput, and help reduce inconsistencies resulting from human error in processing of different plasma samples. We compared 2 techniques for extracting cell-free DNA from maternal plasma: the MagNA Pure™ LC Instrument (Roche Applied Science), an automated DNA isolation system; and a frequently used manual technique involving spin-column technology (High Pure PCR Template Preparation Kit™; Roche Diagnostics).

After approval was granted from the Cantonal Institutional Review Board of Basel, we obtained 9 plasma …
In vitro activities in new oxazolidinone antimicrobial agents against enterococci (1996)

The comparative in vitro activities of two new oxazolidinone antimicrobial agents, U-100592 and U-100766, against 180 isolates of enterococci representing several resistance profiles were examined by using an agar dilution technique. The two oxazolidinones inhibited all isolates, including strains resistant to vancomycin, ampicillin, and minocycline, at concentrations between 1 and 4 micrograms/ml.
Cell Adhesion to Tropoelastin Is Mediated via the C-terminal GRKRK Motif and Integrin αVβ3 (2009)

Elastin fibers are predominantly composed of the secreted monomer tropoelastin. This protein assembly confers elasticity to all vertebrate elastic tissues including arteries, lung, skin, vocal folds, and elastic cartilage. In this study we examined the mechanism of cell interactions with recombinant human tropoelastin. Cell adhesion to human tropoelastin was divalent cation-dependent, and the inhibitory anti-integrin alpha(V)beta(3) antibody LM609 inhibited cell spreading on tropoelastin, identifying integrin alpha(V)beta(3) as the major fibroblast cell surface receptor for human tropoelastin. Cell adhesion was unaffected by lactose and heparin sulfate, indicating that the elastin-binding protein and cell surface glycosaminoglycans are not involved. The C-terminal GRKRK motif of tropoelastin can bind to cells in a divalent cation-dependent manner, identifying this as an integrin binding motif required for cell adhesion.
T Cell Expression Cloning of a Mycobacterium tuberculosis Gene Encoding a Protective Antigen Associated with the Early Control of Infection (2000)

Infection of C57BL/6 mice with Mycobacterium tuberculosis results in the development of a progressive disease during the first 2 wk after challenge. Thereafter, the disease is controlled by the emergence of protective T cells. We have used this infection model in conjunction with direct T cell expression cloning to identify Ags involved with the early control of the disease. A protective M. tuberculosis-specific CD4 T cell line derived from mice at 3 wk postchallenge was used to directly screen an M. tuberculosis genomic expression library. This screen resulted in the identification of a genomic clone comprising two putative adjacent genes with predicted open reading frames of 10 and 41 kDa, MTB10 and MTB41, respectively (the products of Rv0916c and Rv0915c, respectively, in the TubercuList H37Rv database). MTB10 and MTB41 belong to the PE and PPE family of proteins recently identified to comprise 10% of the M. tuberculosis genome. Evaluation of the recombinant proteins revealed that MTB41, but not MTB10, is the Ag recognized by the cell line and by M. tuberculosis-sensitized human PBMC. Moreover, C57BL/6 mice immunized with MTB41 DNA developed both CD4- (predominantly Th1) and CD8-specific T cell responses to rMTB41 protein. More importantly, immunization of C57BL/6 mice with MTB41 DNA induced protection against infection with M. tuberculosis comparable to that induced by bacillus Calmette-Guérin. Thus, the use of a proven protective T cell line in conjunction with the T cell expression cloning approach resulted in the identification of a candidate Ag for a subunit vaccine against tuberculosis.
RMCE-ASAP: a gene targeting method for ES and somatic cells to accelerate phenotype analyses (2006)

In recent years, tremendous insight has been gained on p53 regulation by targeting mutations at the p53 locus using homologous recombination in ES cells to generate mutant mice. Although informative, this approach is inefficient, slow and expensive. To facilitate targeting at the p53 locus, we developed an improved Recombinase-Mediated Cassette Exchange (RMCE) method. Our approach enables efficient targeting in ES cells to facilitate the production of mutant mice. But more importantly, the approach was Adapted for targeting in Somatic cells to Accelerate Phenotyping (RMCE-ASAP). We provide proof-of-concept for this at the p53 locus, by showing efficient targeting in fibroblasts, and rapid phenotypic read-out of a recessive mutation after a single exchange. RMCE-ASAP combines inverted heterologous recombinase target sites, a positive/negative selection marker that preserves the germline capacity of ES cells, and the power of mouse genetics. These general principles should make RMCE-ASAP applicable to any locus.
Remediation of Sleep-Deprivation-Induced Working Memory Impairment with fMRI-Guided Transcranial Magnetic Stimulation (2008)

Repetitive transcranial magnetic stimulation (rTMS) was applied to test the role of selected cortical regions in remediating sleep-deprivation-induced deficits in visual working memory (WM) performance. Three rTMS targets were chosen using a functional magnetic resonance imaging (fMRI)-identified network associated with sleep-deprivation-induced WM performance impairment: 2 regions from the network (upper left middle occipital gyrus and midline parietal cortex) and 1 nonnetwork region (lower left middle occipital gyrus). Fifteen participants underwent total sleep deprivation for 48 h. rTMS was applied at 5 Hz during a WM task in a within-subject sham-controlled design. The rTMS to the upper-middle occipital site resulted in a reduction of the sleep-induced reaction time deficit without a corresponding decrease in accuracy, whereas stimulation at the other sites did not. Each subject had undergone fMRI scanning while performing the task both pre- and postsleep deprivation, and the degree to which each individual activated the fMRI network was measured. The degree of performance enhancement with upper-middle occipital rTMS correlated with the degree to which each individual failed to sustain network activation. No effects were found in a subset of participants who performed the same rTMS procedure after recovering from sleep deprivation, suggesting that the performance enhancements seen following sleep deprivation were state dependent.
A Randomized, Double-Blind, Placebo-Controlled Trial of Buspirone in Combination With an SSRI in Patients With Treatment-Refractory Depression (1998)

Adding buspirone to an SSRI is a safe and well-tolerated drug regimen. This study failed to demonstrate any difference in efficacy between buspirone or placebo augmentation of an SSRI. It could be argued, however, that the study was inconclusive due to the unusually high placebo response.
GPx3 supports ovarian cancer progression by manipulating the extracellular redox environment (2018)

Ovarian cancer remains the most lethal gynecologic malignancy, and is primarily diagnosed at late stage when considerable metastasis has occurred in the peritoneal cavity. At late stage abdominal cavity ascites accumulation provides a tumor-supporting medium in which cancer cells gain access to growth factors and cytokines that promote survival and metastasis. However, little is known about the redox status of ascites, or whether antioxidant enzymes are required to support ovarian cancer survival during transcoelomic metastasis in this medium. Gene expression cluster analysis of antioxidant enzymes identified two distinct populations of high-grade serous adenocarcinomas (HGSA), the most common ovarian cancer subtype, which specifically separated into clusters based on glutathione peroxidase 3 (GPx3) expression. High GPx3 expression was associated with poorer overall patient survival and increased tumor stage. GPx3 is an extracellular glutathione peroxidase with reported dichotomous roles in cancer. To further examine a potential pro-tumorigenic role of GPx3 in HGSA, stable OVCAR3 GPx3 knock-down cell lines were generated using lentiviral shRNA constructs. Decreased GPx3 expression inhibited clonogenicity and anchorage-independent cell survival. Moreover, GPx3 was necessary for protecting cells from exogenous oxidant insult, as demonstrated by treatment with high dose ascorbate. This cytoprotective effect was shown to be due to GPx3-dependent removal of extracellular H<sub>2</sub>O<sub>2</sub>. Importantly, GPx3 was necessary for clonogenic survival when cells were cultured in patient-derived ascites fluid. While oxidation reduction potential (ORP) of malignant ascites was heterogeneous in our patient cohort, and correlated positively with ascites iron content, GPx3 was required for optimal survival regardless of ORP or iron content. Collectively, our data suggest that HGSA ovarian cancers cluster into distinct groups of high and low GPx3 expression. GPx3 is necessary for HGSA ovarian cancer cellular survival in the ascites tumor environment and protects against extracellular sources of oxidative stress, implicating GPx3 as an important adaptation for transcoelomic metastasis.
Syntheses of 1,2,4-benzothiadiazine 1-oxides and 1,2,4-benzothiadiazines (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSyntheses of 1,2,4-benzothiadiazine 1-oxides and 1,2,4-benzothiadiazinesNeville Finch, Salvatore Ricca Jr., Lincoln H. Werner, and Ronald RodebaughCite this: J. Org. Chem. 1980, 45, 17, 3416–3421Publication Date (Print):August 1, 1980Publication History Published online1 May 2002Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/jo01305a009https://doi.org/10.1021/jo01305a009research-articleACS PublicationsRequest reuse permissionsArticle Views322Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts