gebhart/patrepeval huggingface dataset:
Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases. (1988)

The 14-3-3 protein is a family of acidic proteins present exclusively in the brain and is believed to have a function in monoamine biosynthesis because of its ability to activate tyrosine hydroxylase and tryptophan hydroxylase in the presence of Ca2+/calmodulin-dependent protein kinase type II. In this study, we resolved bovine brain 14-3-3 protein into seven polypeptide components by means of reversed-phase chromatography and determined the amino acid sequence of one of these components (eta chain) by cloning its cDNA from a bovine cerebellum cDNA library. The eta-chain mRNA is 1.8 kilobases long and encodes a polypeptide of 246 amino acids and Mr 28,221. Computer-assisted analysis of the sequence indicates that the eta chain exhibits no internal sequence repeats, nor does it have significant sequence similarity to other proteins with known amino acid sequence. However, the eta chain appears to consist of two structural regions that are distinguishable in their clearly different charge characteristics: the almost neutral amino-terminal region and the strongly acidic carboxyl-terminal region. The structural features of the eta chain and the domain organization of tyrosine and tryptophan hydroxylases suggest that the 14-3-3 protein binds to the regulatory domain of the phosphorylated hydroxylases through its acidic carboxyl-terminal region and activates the hydroxylases by inducing an active conformation.
Spontaneous brain magnetic activity in schizophrenia patients treated with aripiprazole. (1998)

This magnetoencaphalographic (MEG) study was conducted as part of a multicenter clinical trial to study the efficacy of aripiprazole. Participants included 5 DSM-IV schizophrenia subjects and 10 age-matched normal controls. The schizophrenia subjects underwent a second MEG recording after 8 weeks of open-label treatment with aripiprazole. Overall, control subjects showed no abnormal spontaneous magnetic brain activity. At washout, 3 patients showed increased delta and theta activity along with paraxosymal bitemporal slow waves. In 2 of these patients, the slow waves were generated in the superior temporal plane, as determined by dipole modeling. In the third patient, the slow waves appeared to have been generated at multiple regions throughout the temporal and inferior parietal lobes. As a group, schizophrenia patients, when compared with normal controls, demonstrated significant decreases in alpha peak frequency and power. Following treatment, aripiprazole had a significant normalizing effect on delta and theta activity. Patients on aripiprazole continued to demonstrate significant abnormalities in alpha frequency and power.
Direct Printing of Circuit Boards Using Aerosol Jet (2011)

Printed Circuit boards (PCBs) are traditionally fabricated using subtractive technologies such as lithography and etching. Discrete passive and active components are typically attached by pick and place and then connected using wire bonding and soldering. In this paper we show that direct printing can replace many of the traditional steps and consequently allow circuits to be fabricated on novel substrates and 3D geometries. Specifically, we report on the integration of Aerosol Jet with the printing of interconnects, passives and COTS attachment. The automated generation of the printed patterns (tool paths) is based on standard Eagle CAD PCB layout software. Multilayer circuits are fabricated by alternately printing metallic wires and a polymeric insulator. Passive components such as resistors and capacitors are printed from a library. The carbon-based resistors have a range of 50 Ω to 1 MΩ and the parallel plate capacitors range from 1 pF to 1 nF. Active devices can be incorporated into the circuit by attaching discrete IC chips with metallic and adhesive inks. The processing temperature is below 150°C for all the printing and curing steps. Overall, Aerosol Jet ® is a cost effective method for directly printing circuits onto non-traditional substrates and is also desirable for prototyping and short-run manufacturing
Optimization of acoustic dispersion for high performance thin film BAW resonators (2006)

The dominant trend in mobile communication is the reduction of cost and size of the components. Bulk-Acoustic-Wave (BAW) filters are ideally suited to replace conventional RF-fil- ters for all major cell phone standards such as GSM, CDMA and WCDMA. In order to achieve excellent filter characteristics it is necessary to get rid of the so called spurious modes. These are undesired resonances due to the finite boundaries of the device. The suppression scheme proposed by us in earlier publications only works if the layer stack fulfills some important requirements. The key issue here is to understand the concept of acoustic layer stack dispersion. It will be shown, that the overlap scheme can only work with a certain dispersion type. For a SMR (surface mounted resonator) it can be shown, that certain stack modifications can influence the dispersion in a desired way, while maintaining most of the other resonator performance parameters. Simulations and interferometrical measurements will be shown, proving this con- cept. It will be shown, that without this stack modification a clean response of a high-Q SMR-type BAW resonator is virtually impos- sible to achieve. BAW; FBAR;Spurious modes; Acoustic dispersion;
Targeting T Cell Metabolism for Improvement of Cancer Immunotherapy (2018)

There has been significant progress in utilizing our immune system against cancer, mainly by checkpoint blockade and T cell-mediated therapies. The field of cancer immunotherapy is growing rapidly but durable clinical benefits occur only in a small subset of responding patients. It is currently recognized that cancer creates a suppressive metabolic microenvironment, which contributes to ineffective immune function. Metabolism is a common cellular feature, and although there has been significant progress in understanding the detrimental role of metabolic changes of the tumor microenvironment (TEM) in immune cells, there is still much to be learned regarding unique targetable pathways. Elucidation of cancer and immune cell metabolic profiles is critical for identifying mechanisms that regulate metabolic reprogramming within the TEM. Metabolic targets that mediate immunosuppression and are fundamental in sustaining tumor growth can be exploited therapeutically for the development of approaches to increase the efficacy of immunotherapies. Here, we will highlight the importance of metabolism on the function of tumor-associated immune cells and will address the role of key metabolic determinants that might be targets of therapeutic intervention for improvement of tumor immunotherapies.
Incorporation of Bevacizumab in the Primary Treatment of Ovarian Cancer (2011)

The use of bevacizumab during and up to 10 months after carboplatin and paclitaxel chemotherapy prolongs the median progression-free survival by about 4 months in patients with advanced epithelial ovarian cancer. (Funded by the National Cancer Institute and Genentech; ClinicalTrials.gov number, NCT00262847.).
Electron Mobility Model of Strained Si<sub>1-x</sub>Ge<sub>x</sub>(001) (2011)

Solving the Schrödinger equation with strain Hamiltonian and combining with KP theory, we obtained the conductivity effective mass and density of states effective mass of strained Si 1-x Ge x (001) in this paper. On the basis of conductivity effective mass and density of states effective mass, considered of Fermi golden rule and Boltzman collision term approximation theory, scattering rate model was established in strained Si 1-x Ge x (001). Based on the conductivity effective mass and scattering rate models we discussed the dependence of electron mobility on stress and doping concentration in strained Si 1-x Ge x (001), it shows that electron mobility decrease with the increasing of stress and doping concentration. This result can provide valuable references to the research of electron mobility of strained Si 1-x Ge x materials and the design of devices.
Elastic Behavior of Methyltrimethoxysilane Based Aerogels Reinforced with Tri-Isocyanate (2010)

The elastic properties and/or flexibility of polymer reinforced silica aerogels having methyltrimethoxysilane (MTMS) and bis(trimethoxysilylpropyl)amine (BTMSPA) making up the silica structure are examined. The dipropylamine spacer from BTMSPA is used both to provide a flexible linking group in the silica structure, and as a reactive site via its secondary amine for reaction with a tri-isocyanate, Desmodur N3300A. The tri-isocyanate provides an extended degree of branching or reinforcement, resulting in increased compressive strength of the aerogel monoliths while the overall flexibility arising from the underlying silica structure is maintained. The compressive moduli of the reinforced aerogel monoliths in this study range from 0.001 to 158 MPa. Interestingly, formulations across this entire range of modulus recover nearly all of their length after two compressions to 25% strain. Differences in pore structure of the aerogels due to processing conditions and solvent are also discussed.
Direct evidence that oxygen-derived free radicals contribute to postischemic myocardial dysfunction in the intact dog. (1989)

Electron paramagnetic resonance (EPR) spectroscopy was used to investigate whether (i) the free radicals produced in the "stunned" myocardium (myocardium with postischemic contractile dysfunction) are derived from O2, (ii) inhibition of radical reactions improves function, and (iii) i.v. spin traps are effective. Open-chest dogs undergoing a 15-min coronary occlusion received an i.v. infusion of the spin trap, alpha-phenyl N-tert-butylnitrone (PBN) (50 mg/kg). In group I (n = 6), EPR signals characteristic of radical adducts of PBN appeared in the coronary venous blood during ischemia and increased dramatically after reperfusion. In group II (n = 6), which received PBN and i.v. superoxide dismutase (SOD; 16,000 units/kg) plus catalase (12,000 units/kg), myocardial production of PBN adducts was undetectable during ischemia (delta = -100%, P less than 0.01 vs. group I) and markedly inhibited after reperfusion (delta = -86%, P less than 0.001). This effect was seen at all levels of ischemic zone flow but was relatively greater in the low-flow range. In group III (n = 8), the same dosages of SOD and catalase without PBN markedly enhanced contractile recovery (measured as systolic wall thickening) after reperfusion [P less than 0.01 at 3 hr vs. controls (group IV, n = 7)]. Systemic plasma activity of SOD and catalase averaged 127 +/- 24 and 123 +/- 82 units/ml, respectively, 2 min after reperfusion. PBN produced no apparent adverse effects and actually improved postischemic contractile recovery in group I (P less than 0.05 at 3 hr vs. controls). This study shows that (i) SOD and catalase are highly effective in blocking free radical reactions in vivo, (ii) the radicals generated in the "stunned" myocardium are derived from univalent reduction of O2, and (iii) inhibition of radical reactions improves functional recovery. The results provide direct, in vivo evidence to support the hypothesis that reactive oxygen metabolites play a causal role in the myocardial "stunning" seen after brief ischemia.
Additional Studies on Flavor Components of Corn Tortilla Chips (1998)

Volatile components from tortilla chips were isolated by high flow dynamic headspace sampling using a closed loop system with excess anhydrous sodium sulfate and trapping the volatiles on Tenax. Capillary GC−MS was used to identify the components. Major volatiles identified included 2-methylpyrazine, 1-hydroxy-2-propanone, 4-vinylguaiacol, 2-acetyltetrahydropyridine, (E,E)- and (E,Z)-2,4-decadienal, acetic acid, 3-methylbutanal, γ-butyrolactone, furfuryl alcohol, and 2,5-dimethylpyrazine. Concentration/threshold ratios indicated that the components that have the highest probability to contribute to the aroma and flavor include (E,E)- and (E,Z)-2,4-decadienal, (E,E)-2,4-nonadienal, 3-methylbutanal, 2-furfurylthiol, 2-acetyl-1-pyrroline, 2-acetyltetrahydropyridine, 2-aminoacetophenone, 4-vinylguaiacol, (E)-2-nonenal, 2-methylbutanal, 2-propionyl-1-pyrroline, (E)-2-decenal, methional, and 2-ethyl-3,5-dimethylpyrazine. Keywords: Corn; tortilla chips; volatiles; flavor; identification; concentrations; odor thresholds
Autocrine Endothelin-3/Endothelin Receptor B Signaling Maintains Cellular and Molecular Properties of Glioblastoma Stem Cells (2011)

Glioblastoma stem cells (GSC) express both radial glial cell and neural crest cell (NCC)-associated genes. We report that endothelin 3 (EDN3), an essential mitogen for NCC development and migration, is highly produced by GSCs. Serum-induced proliferative differentiation rapidly decreased EDN3 production and downregulated the expression of stemness-associated genes, and reciprocally, two glioblastoma markers, EDN1 and YKL-40 transcripts, were induced. Correspondingly, patient glioblastoma tissues express low levels of EDN3 mRNA and high levels of EDN1 and YKL-40 mRNA. Blocking EDN3/EDN receptor B (EDNRB) signaling by an EDNRB antagonist (BQ788), or EDN3 RNA interference (siRNA), leads to cell apoptosis and functional impairment of tumor sphere formation and cell spreading/migration in culture and loss of tumorigenic capacity in animals. Using exogenous EDN3 as the sole mitogen in culture does not support GSC propagation, but it can rescue GSCs from undergoing cell apoptosis. Molecular analysis by gene expression profiling revealed that most genes downregulated by EDN3/EDNRB blockade were those involved in cytoskeleton organization, pause of growth and differentiation, and DNA damage response, implicating the involvement of EDN3/EDNRB signaling in maintaining GSC migration, undifferentiation, and survival. These data suggest that autocrine EDN3/EDNRB signaling is essential for maintaining GSCs. Incorporating END3/EDNRB-targeted therapies into conventional cancer treatments may have clinical implication for the prevention of tumor recurrence.
Correlation of the Kennedy Osteitis Score to clinico‐histologic features of chronic rhinosinusitis (2012)

The KOS is a simple, easy, and reproducible scale in assessing osteitic bones in patients with CRS and can predict measures of severity in eosinophilic rhinosinusitis.
Preparation of Allyl and Vinyl Silanes by the Palladium‐Catalyzed Silylation of Terminal Olefins: A Silyl‐Heck Reaction (2012)

Installing silicon is easy! A high-yielding protocol for the palladium-catalyzed silylation of terminal alkenes is reported. This method allows facile conversion of styrenes to E-β-silyl styrenes by using iodotrimethylsilane (TMSI) or chlorotrimethylsilane/lithium iodide (see scheme). Terminal allyl silanes with good E/Z ratios are also readily accessed from α-olefins.
An XQuery engine for digital library systems that support XML data (2004)

XML has become widely accepted for both representation and exchange of data over the Internet. This situation urges digital library systems to deal with XML documents as well as typical text documents. A standard query language is very helpful for interoperability among digital library systems over the Internet. XQuery is a forthcoming standard for querying XML data. We propose an XQuery engine that can be used as an XQuery processing module in digital library systems that support XML documents. Our system has the following useful aspects. First, any user interface that generates XQuery is able to access any digital library systems embedding our XQuery engine. Second, we define primitive operation trees (POTs) so that they can become a standard interface between an XQuery engine and an information retrieval engine. Third, some query optimizations over POTs can be done in the XQuery engine so that better searching performance is expected.
SCH 58261: A selective A2A adenosine receptor antagonists (1997)

The recent discovery of selective antagonists for the A2A adenosine receptors has been of great help to further research in this field. One compound, SCH 58261, 5- amino-7-(β-phenylethyl)-2-(8-furyl)pyrazolo[4,3-e]-1,2,4-triazolo[1,5-c]pyrimidine, is playing a key part in a variety of studies. This review describes its pharmacological characteristics as they are emerging in various laboratories. The compound has an affinity in the low nM range (Ki value of 1–2 nM) for A2A receptors located on membranes from a variety of tissues and cell types, including rat and bovine brain striatum, human platelets, lymphocytes and neutrophils, porcine coronary arteries, and CHO cells transfected with the cloned human A2A receptors. SCH 58261 has little or no affinity up to the μM range for adenosine A2B, A3, or other G protein-coupled receptors. Selectivity for A2A vs. A1 receptors varies from 53- to 750-fold, depending on membranes or type of assay. SCH 58261 blocks A2A-receptor mediated increase of cyclic AMP formation with high potency (e.g., IC50 values between 15 and 20 nM in human white blood cells). The tritiated form of SCH 58261 specifically labels A2A receptors in discrete regions of the rat brain such as caudate-putamen, nucleus accumbens, and tuberculum olfactorium. In classic in vitro bioassays, such as A2A-receptor-mediated vasodilation in coronary arteries, SCH 58261 displays competitive antagonistic properties (e.g., pA2 value of 9.5 in porcine coronary arteries). In assays involving responses mediated by A1 or A2B receptors, SCH 58261 shows little or no activity up to concentrations about 100-fold higher than those affecting A2A receptors (higher concentrations not being testable due to its poor water solubility). In the rat, SCH 58261 enhances locomotor activity (at 3.7 mg/kg ip), increases wakefulness (10 mg/kg ip) and slightly increases both blood pressure and heart rate (10 mg/kg ip). These activities appear to be specifically mediated by A2A receptors since the drug counteracts the effect of A2A receptor agonists in some experimental paradigms. In models mimicking CNS disorders, SCH 58261 potentiates the activity of L-DOPA or dopamine receptor agonists in the 6-hydroxydopamine-lesioned rat model. Moreover, the compound reduces brain infarct size in a rat model of cerebral ischemia. Altogether, SCH 58261 and its radiolabeled form have emerged as interesting tools for better understanding the function of A2A receptors in physiological or altered conditions. Moreover, the neuroprotective properties of SCH 58261 indicate that drugs of this class have a potential for treatment of brain damage produced by Parkinson's disease or stroke. Drug Dev. Res. 42:63–70, 1997. © 1997 Wiley-Liss, Inc.
Detection of non-A, non-B hepatitis antigen by immunocytochemical staining. (1984)

Liver tissue obtained from a chimpanzee during the acute phase of an experimental non-A, non-B (NANB) hepatitis virus infection was studied by a sensitive immunocytochemical staining procedure for the presence of NANB viral antigens. Initial investigations were conducted with a model system of hepatitis B virus (HBV) antigens for purposes of comparing two immunocytochemical staining methods. Of these two procedures, an immunoperoxidase procedure, utilizing an avidin-biotinylated enzyme complex, was at least 40-fold more sensitive than a conventional immunoperoxidase technique for the detection of HBV-specific tissue antigens. Utilization of the avidin-biotin-amplified immunoperoxidase staining procedure, in conjunction with four primary convalescent antisera obtained from NANB hepatitis-implicated donors, resulted in the observation of NANB virus-associated antigen in the cytoplasm of hepatocytes from an infected chimpanzee liver. These same human antisera were not reactive with a number of uninfected control cells nor with cells infected with HBV, hepatitis A virus, or cytomegalovirus. Preincubation of one of these convalescent NANB sera, or IgG derived thereof, with an acute-phase serum obtained from a NANB hepatitis virus-infected chimpanzee abolished the antibody reactivity. We conclude from these observations that selected convalescent sera from NANB hepatitis virus-infected patients contain low levels of antibody that specifically react with a cytoplasmic antigen associated with NANB virus-infected hepatocytes.
NMR Study of the Interaction between the B Domain of Staphylococcal Protein A and the Fc Portion of Immunoglobulin G (1998)

The solution structure of the B domain of staphylococcal protein A (FB) complexed with the Fc fragment of immunoglobulin G (IgG) is reported. A previous NMR analysis has shown that in solution FB is composed of a bundle of three alpha-helices, helix I, helix II, and helix III [Gouda, H., Torigoe, H., Saito, A., Sato, M., Arata, Y., and Shimada, I. (1992) Biochemistry 31, 9665-9672]. In contrast, the crystal structure of FB in the FB-Fc complex lacks helix III. Uniformly 15N- and 15N/13C-labeled FB were prepared, and the backbone 13C resonances were assigned. The spectral data obtained in the present study indicated that in solution all three helices including helix III are preserved in the FB-Fc complex. The mode of interaction of FB with the Fc fragment was discussed on the basis of the combined data of hydrogen-deuterium exchange experiments and 1H-15N correlation spectroscopy. It was concluded that a contiguous surface shaped by F14, Y15, E16, L18, and H19 in helix I, and N29, Q33, L35, and K36 in helix II is responsible for the binding.
Record linkage (2006)

This tutorial provides a comprehensive and cohesive overview of the key research results in the area of record linkage methodologies and algorithms for identifying approximate duplicate records, and available tools for this purpose. It encompasses techniques introduced in several communities including databases, information retrieval, statistics and machine learning. It aims to identify similarities and differences across the techniques as well as their merits and limitations.
Chitosan microparticles loaded with yeast-derived PCV2 virus-like particles elicit antigen-specific cellular immune response in mice after oral administration (2014)

Chitosan microparticles appear to be a safe, simple system on which to base PCV2 oral vaccines. Oral chitosan-mediated antigen delivery is a novel strategy that efficiently induces anti-PCV2 cellular responses in a mouse model. Further studies in swine are warranted.
Isolation of scFvs to In Vitro Produced Extracellular Domains of EGFR Family Members (2005)

The members of the epidermal growth factor receptor (EGFR) family are over expressed in a variety of malignancies and are frequently linked to aggressive disease and a poor prognosis. Although clinically effective monoclonal antibodies (MAbs) have been developed to target HER2 and EGFR, the remaining two family members, HER3 and HER4, have not been the subject of significant efforts. In this paper, we have taken the initial steps required to generate antibodies with potential clinically utility that target the members of the EGFR family. The genes for the extracellular domains (ECDs) of all four members of the EGFR family were cloned and used to stably transfect 293 (HEK) cells. Milligram quantities of each ECD were produced and characterized. The HER3, HER4, and EGFR ECDs were then employed as targets for the selection of antibodies from naïve human scFv (single-chain Fv) phage display libraries. Six unique scFv clones were isolated that bound specifically to HER3, 13 unique clones were isolated with specificity for HER4 and 52 unique anti-EGFR clones were isolated. These scFvs provide a valuable and potentially clinically relevant panel of agents to target the members of the EGFR family.
Sedation with nasal ketamine and midazolam for cryotherapy in retinopathy of prematurity. (1993)

Sedation of infants in ophthalmology is a challenge for the anaesthetist. Often general anaesthesia is not required but a quiet and immobile patient is necessary ifthe examination/ treatment is to be successful.