gebhart/patrepeval huggingface dataset:
Pretreatment of donor mice with granulocyte colony-stimulating factor polarizes donor T lymphocytes toward type-2 cytokine production and reduces severity of experimental graft-versus-host disease (1995)

The incidence and severity of acute graft-versus-host disease (GVHD) after allogeneic transplantation using peripheral blood progenitor cells mobilized by granulocyte colony-stimulating factor (G-CSF) appear to be no worse than those after bone marrow transplantation, despite the presence of large numbers of T cells in the donor infusion. Experimental studies have shown that type-1 T cells (secreting interleukin-2 [IL-2] and interferon-gamma) mediate acute GVHD, whereas type-2 T cells (secreting IL-4 and IL-10) can prevent acute GVHD. We tested the hypothesis that G-CSF modulates T-cell function toward a type-2 response and thus reduces the severity of acute GVHD. B6 mice were injected with G-CSF or diluent for 4 days, and their splenic T cells were stimulated in vitro with alloantigen or mitogen in the absence of G-CSF. T cells from G-CSF-treated mice showed a significant increase in IL-4 production, with a simultaneous decrease in IL-2 and interferon-gamma production in response to both stimuli. We also examined the effect of G-CSF pretreatment of donors in a GVHD model (B6-->B6D2F1). Survival was significantly improved in recipients of G-CSF-treated donors. Concanavalin-A-induced cytokine production at day 13 after transplantation also showed an increase in IL-4 along with a decrease in IL-2 and IFN-gamma production by splenocytes from recipients of G-CSF-treated bone marrow and T cells. These data show that pretreatment of donors with G-CSF polarizes donor T cells toward the production of type-2 cytokines, which is associated with reduced type-1 cytokine production and reduced severity of acute GVHD.
Novel Lipid System Forming Hollow Microtubes at High Yields and Concentration (2006)

There is considerable interest in constructing supramolecular hollow tube architectures based on amphiphilic molecules. This can be achieved by using relatively expensive synthetic lipids. Herein, we report on the facile preparation of self-assembled microtubes from a novel low-cost lipid mixture that does not require a previous chemical synthesis step and consists of the ethanolamine salt of 12-hydroxy-stearic acid in water. Tubes of more than 10 microm in length spontaneously form upon cooling from an isotropic solution. They exhibit inner and outer diameters of 400 and 600 nm, respectively, and their walls consist of concentric stacked bilayers of fatty acid salts, each separated by a layer of water.
Secretase inhibitors for Alzheimer's disease: challenges of a promiscuous protease. (2005)

The proteolytic enzyme gamma-secretase cleaves amyloid precursor protein (beta-APP), following beta-secretase cleavage to generate the amyloid-beta peptides that are causally linked to Alzheimer's disease (AD). However, gamma-secretase is also responsible for intramembranous cleavage of a growing list of additional transmembrane proteins, and therefore therapeutic inhibition of gamma-secretase might also affect these substrates. Such blockade over a chronic period may be deleterious, due to interference with potential cell signaling pathways activated by any of the products of these novel gamma-secretase substrates. In addition, inhibition of gamma-secretase leads to alterations in other beta-APP metabolites, with potential toxicity and signaling implications. The potential consequences of these off-target effects of gamma-secretase inhibitors are reviewed.
Both physical fitness and acute exercise regulate nitric oxide formation in healthy humans (1997)

We analyzed nitrate, a major stable end product of nitric oxide (NO) metabolism in vivo in plasma and urine from groups of healthy subjects with different working capacities. Resting plasma nitrate was higher in athletic subjects than in nonathletic controls [45 +/- 2 vs. 34 +/- 2 (SE) microM; P < 0.01]. In other subjects, both the resting plasma nitrate level (r = 0.53; P < 0.01) and the urinary excretion of nitrate at rest (r = 0.46; P < 0.01) correlated to the subjects' peak work rates, as determined by bicycle ergometry. Two hours of physical exercise elevated plasma nitrate by 18 +/- 4 (P < 0.01) and 16 +/- 6% (P < 0.01), respectively, in athletes and nonathletes, compared with resting nitrate before exercise. We conclude that physical fitness and formation of NO at rest are positively linked to each other. Furthermore, a single session of exercise elicits an acute elevation of NO formation. The observed positive relation between physical exercise and NO formation may help to explain the beneficial effects of physical exercise on cardiovascular health.
Query processing of streamed XML data (2002)

We are addressing the efficient processing of continuous XML streams, in which the server broadcasts XML data to multiple clients concurrently through a multicast data stream, while each client is fully responsible for processing the stream. In our framework, a server may disseminate XML fragments from multiple documents in the same stream, can repeat or replace fragments, and can introduce new fragments or delete invalid ones. A client uses a light-weight database based on our proposed XML algebra to cache stream data and to evaluate XML queries against these data. The synchronization between clients and servers is achieved through annotations and punctuations transmitted along with the data streams. We are presenting a framework for processing XML queries in XQuery form over continuous XML streams. Our framework is based on a novel XML algebra and a new algebraic optimization framework based on query decorrelation, which is essential for non-blocking stream processing.
44.2: Novel Front‐light System Using Fine‐pitch Patterned OLED (2008)

Abstract We have developed a novel front‐light system that uses an organic light emitting diode (OLED) light source patterned with a fine pitch. The front‐light system has the following characteristics: 1. Excellent uniformity within the light emitting area; 2. Emittance that is consistent at all viewing angles; 3. No light leakage at any viewing angle from the side of the observer. This system can be adopted for reflective LCDs, electrophoretic displays (EPD), microelectromechanical systems (MEMS) and other applications.
Recombinant human granulocyte-macrophage colony-stimulating factor reduces hematologic toxicity and widens clinical applicability of high-dose cyclophosphamide treatment in breast cancer and non-Hodgkin's lymphoma. (1990)

High-dose administration of anticancer agents is attractive both on theoretic and clinical grounds. Yet, high-dose regimens are usually used as salvage treatments, mainly as a consequence of their considerable hematologic toxicity. One pertinent example is represented by cyclophosphamide, an alkylating agent with a wide spectrum of marked antitumor activity. When used at doses up to 7 g/m2 (190 to 200 mg/kg) this drug does not cause myeloablation, but induces a severe, albeit transient, myelosuppression, which requires platelet transfusions in approximately 50% of treated patients, and is frequently complicated by infectious episodes, occasionally lethal. To accelerate hematopoietic recovery, we continuously infused for 14 consecutive days 5.5 micrograms/kg/d of the glycosylated human recombinant granulocyte-macrophage colony-stimulating factor (rhGM-CSF) into 15 patients with breast cancer and non-Hodgkin's lymphoma treated with 7 g/m2 cyclophosphamide. This schedule was chosen having obtained the fastest hematopoietic recovery among four different options during an initial schedule-finding phase on 12 overall patients. Twenty-one comparable subjects with solid tumors served as controls. We report here that this relatively low, well-tolerated dose of rhGM-CSF reduces from 20 to 14 (median) and from 24 to 14, the number of days required to recover circulating granulocyte counts over 1,000 and 2,500/microL, respectively. The stimulatory effect was associated with a remarkable clinical benefit. In fact, treated patients experienced less infectious complications (7% v 24%) were eligible to receive chemotherapy earlier (median, by day +14 v day +20 for controls), and fewer required prophylactic platelet transfusions (13% v 43%). Our results show that even very high doses of cyclophosphamide can be administered with improved hematologic toxicity, tolerable morbidity, and reduced supportive care requirements. The increase in the therapeutic index made possible by rhGM-CSF infusion prompts the use of high-dose cyclophosphamide, and possibly of other agents with similar myelotoxic activity, early in the clinical course of chemotherapy-sensitive tumors.
Role of SOX2 in maintaining pluripotency of human embryonic stem cells (2010)

Human embryonic stem cell (ESC) pluripotency is thought to be regulated by several key transcription factors including OCT4, NANOG, and SOX2. Although the functions of OCT4 and NANOG in human ESCs are well defined, that of SOX2 has not been fully characterized. To investigate the role of SOX2, we modulated the level of SOX2 expression in human ESCs. Reduction of SOX2 expression in human ESCs induced trophectodermal and partial endodermal differentiation. Interestingly, CDX2, a typical trophectoderm-associated gene, was not up-regulated. In contrast, using the Tet-on gene inducible system, SOX2 over-expression in human ESCs induced trophectoderm differentiation accompanied by increased CDX2 expression. Additionally, SOX2 over-expression resulted in an increase in CGalpha-positive cells, which marks later stage trophectoderm development, rather than placental lactogen-positive cells. Thus, over-expression as well as repression of SOX2 expression in human ESCs resulted in their differentiation into the trophectoderm lineage. Our data show that SOX2 plays an important role in the maintenance of pluripotency of human ESCs and possibly, trophoblast development.
Role of Galectin-3 in Human Pulmonary Fibrosis (2007)

These results suggest that galectin-3 is involved in the pathogenesis of human IPF and CVD-IP by activating macrophages and fibroblasts.
New Insights into Structure and Luminescence of EuIII and SmIII Complexes of the 3,4,3-LI(1,2-HOPO) Ligand (2015)

We report the preparation and new insight into photophysical properties of luminescent hydroxypyridonate complexes [M(III)L](-) (M = Eu or Sm) of the versatile 3,4,3-LI(1,2-HOPO) ligand (L). We report the crystal structure of this ligand with Eu(III) as well as insights into the coordination behavior and geometry in solution by using magnetic circular dichroism. In addition TD-DFT calculations were used to examine the excited states of the two different chromophores present in the 3,4,3-LI(1,2-HOPO) ligand. We find that the Eu(III) and Sm(III) complexes of this ligand undergo a transformation after in situ preparation to yield complexes with higher quantum yield (QY) over time. It is proposed that the lower QY in the in situ complexes is not only due to water quenching but could also be due to a lower degree of f-orbital overlap (in a kinetic isomer) as indicated by magnetic circular dichroism measurements.
Increasing methylation of the CDKN2A gene is associated with the progression of adult T-cell leukemia. (2000)

In this study, we examined the methylation status of the CDKN2A gene in patients with different forms of adult T-cell leukemia (ATL) using Southern blot analysis, methylation-specific PCR (MSPCR), and nucleotide sequencing. We found that the CDKN2A gene was more frequently methylated in fresh tumor cells isolated from patients with acute ATL (47%) or lymphoma-type ATL (73%) than in those with less malignant chronic (17%) and smoldering (17%) ATL. In addition, deletions of the CDKN2A gene were found in 24% of acute ATL patients; thus, abnormalities of the CDKN2A gene totaled 71% in acute ATL patients. In contrast, no CDKN2A gene methylation was found in asymptomatic carriers or uninfected individuals. Methylation of the p15 gene was not found in any samples from 36 ATL patients. Direct sequencing of the CDKN2A gene after sodium bisulfite treatment of genomic DNA revealed that the methylation of CpG sites had occurred in 24 of 32 ATL cases (75%) including chronic and smoldering ATL, even when MSPCR and the Southern blot had failed to detect CDKN2A gene methylation. Among fresh ATL samples with methylation, methylation was detected in the promoter region and exon in 17 of 24 cases, and methylation in the exon without promoter region was detected in 7 of 24 cases. In one case, the pattern of methylation proved to be different between peripheral blood cells and lymph node cells, suggesting the presence of multiple subclones with regard to methylation patterns, despite the same HTLV-I integration site. Quantitative PCR showed a marked decrease in CDKN2A mRNA expression in the cells with a methylated CDKN2A gene, especially if the promoter region was methylated. These findings suggest that CpG methylation decreases CDKN2A expression and represents a critical factor in the disease progression of ATL.
Synthesis and Evaluation of Phenylalanine-Modified Hyperbranched Poly(amido amine)s as Promising Gene Carriers (2009)

Hyperbranched poly(amido amine) (HPAMAM), which is structurally analogous to PAMAM dendrimers, has been proposed to be an effective agent for gene delivery. The facile synthesis of HPAMAM with scalable productivity by one-pot polymerization of monomers of methyl acrylate (MA) and diethylenetriamine (DETA) has been set up previously. In this study, the HPAMAM was further modified on the terminal amino groups with phenylalanine to various degrees (HPAMAM-PHE30, PHE45, PHE60). We showed that HPAMAM and HPAMAM-PHEs were all able to form complexes with plasmid DNA (pDNA) at various mass ratios. The cytotoxicity and transfection efficiencies of these polymers were evaluated in SMMC-7721 and COS-7 cell lines. The PHE modifications affected the cell transfection efficiency significantly. The HPAMAM-PHE60 was the most efficient, with transfection activities consistently higher than the commercial transfection reagent PEI. Our study demonstrated that HPAMAM-PHEs may be good new materials for gene delivery and other applications because of its large-scale availability, economical cost, and low toxicity.
Lifetime exposure to radiation from imaging investigations. (2006)

The current lifetime level of radiation to which patients are exposed by diagnostic imaging appears to be far below the maximum recommended level. Some patients do exceed the maximum recommended annual level, but this overexposure is generally warranted due to serious medical illness or injury, and the benefit outweighs the risk. We found no evidence of an association between these low levels of radiation and development of cancer.
Neutrophils, host defense, and inflammation: a double-edged sword (1994)

Neutrophils play important roles in host defense against all classes of infectious agents but, paradoxically, they are also involved in the pathology of various inflammatory conditions. Their microbicidal armory consists of oxidative and nonoxidative processes that are activated simultaneously upon phagocytosis. Although destruction of infectious agents occurs intracellularly, release of cytotoxic molecules into the extracellular milieu can damage body tissues. Neutrophils are heterogeneous. Subpopulations exist in various stages from dormant to primed to fully activated. The activities of neutrophils are regulated locally in microenvironments and systemically by a plethora of mediators including cytokines, "classical" neuroendocrine hormones, and bioactive lipids. The net response depends on a complex balance of stimulatory and inhibitory pathways that are regulated by these mediators. Although some effector and regulatory pathways are vital, considerable redundancy is also evident. Identification of the essential mediators and the unraveling of any interactions may be the keys to understanding the neutrophil paradox and developing therapeutic strategies that optimize microbial killing and minimize host tissue damage. Finally, reports that neutrophils can act as drug delivery vectors and that their function is influenced by stress and other lifestyle factors suggest that new homeostatic functions for these cells, outside their traditional roles in host defense and inflammation, remain to be identified: some are speculated on here.
Adenoviral Gene Therapy Leads to Rapid Induction of Multiple Chemokines and Acute Neutrophil-Dependent Hepatic Injury in Vivo (1999)

Replication-deficient adenoviruses are known to induce acute injury and inflammation of infected tissues, thus limiting their use for human gene therapy. However, molecular mechanisms triggering this response have not been fully defined. To characterize this response, chemokine expression was evaluated in DBA/2 mice following the intravenous administration of various adenoviral vectors. Administration of adCMVbeta gal, adCMV-GFP, or FG140 intravenously rapidly induced a consistent pattern of C-X-C and C-C chemokine expression in mouse liver in a dose-dependent fashion. One hour following infection with 10(10) PFU of adCMVbeta gal, hepatic levels of MIP-2 mRNA were increased >60-fold over baseline. MCP-1 and IP-10 mRNA levels were also increased immediately following infection with various adenoviral vectors, peaking at 6 hr with >25- and >100-fold expression, respectively. Early induction of RANTES and MIP-1beta mRNA by adenoviral vectors also occurred, but to a lesser degree. The induction of chemokines occurred independently of viral gene expression since psoralen-inactivated adenoviral particles produced an identical pattern of chemokine gene transcription within the first 16 hr of administration. The expression of chemokines correlated as expected with the influx of neutrophils and CD11b+ cells into the livers of infected animals. At high titers, all adenoviral vectors caused significant hepatic necrosis and apoptosis following systemic administration to DBA/2 mice. To investigate the role of neutrophils in this adenovirus-induced hepatic injury, animals were pretreated with neutralizing anti-MIP-2 antibodies or depleted of neutrophils. MIP-2 antagonism and neutrophil depletion both resulted in reduced serum ALT/AST levels and attenuation of the adenovirus-induced hepatic injury histologically, confirming that this early injury is largely due to chemokine production and neutrophil recruitment. Our findings further clarify the early immune response against replication-deficient adenoviral vectors and suggest a strategy to prevent adenovirus-mediated inflammation and tissue injury by interfering with chemokine or neutrophil function.
IDENTIFICATION OF THE ANTIBODY TO VASCULAR ENDOTHELIAL CELLS IN PATIENTS UNDERGOING CARDIAC TRANSPLANTATION (1985)

Acute cardiac dysfunction occurred in four cardiac allograft recipients with negative donor-specific lymphocyte crossmatches. In two recipients the transplanted heart was removed and the patients were maintained on bypass for several hours until a second cardiac allograft was available. In these patients the second transplanted heart also underwent acute dysfunction. The lymphocyte crossmatch was again negative in both second transplants. Two of the four recipients had no detectable antibody to a panel of lymphocytes. Examination of the hearts demonstrated histologic findings consistent with hyperacute rejection. Direct immunofluorescence performed on the transplanted hearts revealed the presence of immunoglobulin and complement deposited on the vascular endothelium. Pathology data was available on 3 of the 4 patients who experienced acute cardiac dysfunction. Pretransplant sera from these four recipients were screened for the presence of antivascular endothelial cell (VEC) antibody. The sera from all four recipients were found to contain antibody against an endothelial cell panel. In addition, donor-specific aorta and vena cava were available from one of the heart donors. The recipient was found to have donor-specific antibody to VEC. Thus, antibody directed against VEC specific antigens appears to be related to hyperacute rejection of heart allografts.
Corrosion behaviour of gold surfaces protected with bonded perfluoro polyethers (2004)

Abstract Perfluoro polyethers (PFPE) are known to offer some protection against the corrosion occurring on electroplated gold surfaces used in electrical contact applications. In this field, reliability requirements and severity of the environment of use are permanently increasing. We describe here the results of corrosion tests performed on electroplated gold coupons protected with layers of various PFPE. XPS measurements show that functionalized PFPE can be grafted to the surface. Attempts to bond the PFPE layer to the surface were also performed by exposure to UV light. Lubricant layers are shown to have better properties of protection against corrosion when they are bonded to the surface. Correlation with the mechanical and electrical performances required from such layers is also discussed. Copyright © 2004 John Wiley &amp; Sons, Ltd.
Comparison of Six Cropping Systems for Yellow Nutsedge (Cyperus esculentus) Control (1983)

Six cropping/weed control systems were evaluated from 1978 to 1980 for the control of yellow nutsedge ( Cyperus esculentus L.). Supplementing cultivation of cotton ( Gossypium hirsutum L. ‘Acala SJ-2′) with either preplant applications of fluridone {1 - methyl - 3 - phenyl - 5 - [3 - (trifluoromethyl)phenyl] -4(1 H -pyridinone} or two hoeings for 2 yr preceding cotton treated with DSMA (disodium methanearsonate) and MSMA (monosodium methanearsonate) reduced populations of viable yellow nutsedge tubers 98 to 99% within 3 yr. Dry- or wet - fallowing plus tillage after barley ( Hordeum vulgare L. ‘Kombyne’), and double cropping potatoes ( Solanum tuberosum L. ‘White Rose’) treated with EPTC ( S -ethyl dipropylthiocarbamate) with soybeans [ Glycine max (L.) Merr. ‘Williams’] treated with alachlor [2-chloro - 2′, 6’ - diethyl - N - (methoxymethyl)acetanilide] for 2 yr preceding cotton, reduced populations of tubers 98 to 99% within 3 yr. A similar reduction of tubers (97%) was obtained by double cropping potatoes with milo [ Sorghum bicolor (L.) Moench. ‘NK- 265′] for 2 yr preceding cotton.
Coat Protein-Mediated Resistance Against Virus Infection (1990)

The common phenotype of different resistant plants are described and individual examples are reviewed in order to compare and contrast the characteristics of resistance to different viruses. The limited number of experiments that explore the fundamental basis of resistance are described. Ideas are presented for the types of experiments that are needed to more completely understand the mechanisms of resistance and to extend the resistance to other important plant viruses. Topics covered include: definition; strategy to create coat protein-mediated resistance; phenotype of coat protein-mediated resistance; examples of coat protein-mediated resistance; understanding the mechanisms of CP-mediated resistance; field tests of coat protein-mediated resistance; and conclusions and future directions
Possible Antifertility Agents. Part-I. Synthesis of 2-(N,N-Substituted-aminomethyI )-3- (2-pyridyl)-4(3H)-oxo-3, 1-quinazolines (1984)

Department of Chemistry, University of Lucknow, Lucknow-226 007 <em>Manuscript received 3 September 1983, revised 2 June 1984, accepted 17 June 1984</em> Possible Antifertility Agents. Part-I. Synthesis of 2-(N,N-Substituted-aminomethyl)-3- (2-pyridyl)-4(3H)-oxo-3, 1-quinazolines
Reversible Cerebral Hypoperfusion Observed With Tc-99m HMPAO SPECT in Reversible Dementia Caused by Hypothyroidism (1999)

A 69-year-old man had hypothyroid dementia as a result of I-131 therapy and an overdose of methimazole. Tc-99m HMPAO SPECT revealed diffuse cerebral hypoperfusion. The findings of brain SPECT normalized with the disappearance of symptoms and a return to the euthyroid state. There was a 25% or 26% reduction of the mean cerebral blood flow during dementia. This may be the first report in which SPECT brain imaging revealed reversible hypoperfusion associated with reversible hypothyroid dementia.