gebhart/patrepeval huggingface dataset:
Amino acid sequence of monkey amyloid protein A (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmino acid sequence of monkey amyloid protein AMark A. Hermodson, Robert W. Kuhn, Kenneth A. Walsh, Hans Neurath, Nils Eriksen, and Earl P. BendittCite this: Biochemistry 1972, 11, 16, 2934–2938Publication Date (Print):August 1, 1972Publication History Published online1 May 2002Published inissue 1 August 1972https://doi.org/10.1021/bi00766a002RIGHTS & PERMISSIONSArticle Views46Altmetric-Citations69LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (502 KB) Get e-Alerts Get e-Alerts
Improvements in laser welding of chicken bone tibias in vitro (1995)

Techniques are being developed to `tack weld' broken bones together with the help of a laser. These techniques would, in some instances, allow doctors to avoid the use of foreign fixation devices such as metal plates and pins for the repair of small bones as in the delicate maxillofacial and nasal bones. Successful laser `welding' of chicken tibias in vitro was reported by the authors last year. Since that time, parameters affecting the strength of the weld have been examined. Several different compositions have been tested as possible `solders' for the welding process. These include substances such as reagent grade gelatin and a hydroxyapatite-based cement. The bones welded with the gelatin mix and subsequently refrigerated have been consistently stronger than those welded without gelatin. The hydroxyapatite-based cement did not increase weld strength. The effect of the concentration of a demineralization solution, hydrochloric acid, has also been examined. Lower pH values correspond to stronger welds. The tensile strength of the weld has also been shown to depend on the extent of hydration during the 24 hours after laser welding.
Fenomenologia trascendental y ontologia (1990)

La tesis doctoral de don agustin serrano de haro lleva a
cabo una exposicion de los fundamentos de la
fenomenologia husserliana, en especial de la
fenomenologia trascendental, desde un punto de vista
ontologico. La perspectiva que asume el trabajo es, mas
en concreto, la de la teoria general de los todos y las
partes que husserl desarrolla en la tercera
investigacion. El autor cree poder mostrar que la
fenomenologia trascendental nace como una superacion de
los principios basicos de esta teoria general, del
principio de que cualquier parte efectica de un todo es
una parte ingrediente en el y del principio correlativo
de que el todo es una complexion de contenidos
ingredientes. La conciencia pura como correlacion
noetico-noematica incluiria precisamente componentes que
se definen por no ser ingredientes al todo (a saber: Los
noematicos) y el todo de la conciencia no respondera por
tanto a la forma de una complexion, sino a la de una
indisoluble correlacion.
Myelination and remyelination of aggregate rat brain cell cultures enriched with macrophages (1997)

We reported previously that accumulation of myelin basic protein (MBP) in foetal brain aggregate cultures is enhanced by supplementation with peritoneal macrophages. The present study demonstrates that the rate of MBP accumulation in macrophage-enriched cultures continues to increase over time unaccompanied by a matching increase in the oligodendrocyte marker cyclic nucleotide phosphodiesterase, while that of control cultures reaches a plateau. These observations are supported by electron microscopic evidence of cumulative numbers of myelinated axons in the aggregates over time and by enhanced expression of myelin protein genes in macrophage-enriched relative to control cultures. Aggregates demyelinate following short-term exposure to cytokines and antimyelin oligodendrocyte glycoprotein antibody, and MBP synthesis resumes following removal of demyelinating agents. Supplementation of cultures with macrophages influences the degree of myelin breakdown and remyelination, drawing attention to the role that macrophage-derived growth factors may play in myelinogenesis and myelin repair in inflammatory demyelinating disease.
Can novice drivers be trained to scan for information that will reduce their likelihood of a crash? (2006)

Sixteen year old drivers are involved in 10.3 fatal crashes per 100 million vehicle miles, a rate almost double that of 18 year olds and almost eight times that of 45-64 year olds, who are the safest group of drivers. Crash rates are particularly higher during the first month of licensure and decline rapidly for about six months and 1000 miles and then much more slowly for at least two years, consistent with a typical learning curve. Research indicates that drivers who have their learner's permit or are just newly licensed have particular difficulties identifying areas of a scenario from which hidden risks could emerge. Standard driver education programs do not appear to address these difficulties adequately. This suggests that some alternative form of driver training could reduce the crashes, either in the classroom or on the road. A PC based program designed to teach drivers to recognize risks early on is shown to improve their awareness of hazards, both on an advanced driving simulator and on the road.
Drug Delivery Systems (2017)

This chapter describes the Food and Drug Administration (FDA's) origin and its organization, followed by a description of some of the standards that have been either adopted or developed to address critical technical issues in drug delivery systems (DDS). It provides an overview of the FDA regulatory pathways involved in the regulation of DDS and a brief overview on micro- and nanotechnologiesm which are predicted to play a critical role in the future of DDS. One of the most important issues in drug therapy is the route of administration that determines the onset of action of the drug and some side effects that are specifically related to each route. Several routes of administration are available in the human body with an amazing repertoire of DDS developed for each one of them. A first classification of DDS is based on the different timing/rate of release, dividing oral DDS into immediate-release and modified-release DDS.
Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28 (1995)

The expression of the murine T cell Ag 4-1BB, a member of the TNF-R family, is induced by T cell activation. Previously, we and others had shown that signaling through 4-1BB enhanced proliferative T cell responses. To investigate a potential role for the interaction of 4-1BB with its ligand (4-1BBL) in T cell activation, we studied the ability of a soluble chimera of 4-1BB (4-1BBFc) to interfere with proliferative responses and cytokine production in models of activation dependent in intercellular interactions. The potential blocking effect of 4-1BBFc was compared with that of the chimeric molecule CTLA-4Ig, a reagent known to interfere with the interaction of CD28 (and/or CTLA-4) with B7 costimulatory receptors. In this study, we report that 4-1BBFc partially blocked both the activation of unfractionated splenocytes triggered by soluble anti-CD3 (anti-CD3s), and the more physiologically relevant responses to alloantigen. In addition, we show that both chimeric molecules partially blocked proliferative responses and IL-2 secretion by highly purified resting T cells activated with anti-CD3s in the presence of fixed accessory cells that express B7 receptors and 4-1BBL. Furthermore, in this model system, the blocking capacity of 4-1BBFc and CTLA-4Ig appears to correlate with the relative expression of their respective cognate receptors (4-1BBL and B7) on the accessory cell. Simultaneous addition of both blocking reagents produced an additive effect in the model systems studied.
Microfracture Surface Characterizations: Implications for In Situ Remedial Methods in Fractured Rock (2006)

Agreement No. CR-827878-01-0 to the USEPA. It has been subjected to the Agency’s peer and administrative review and has been approved for publication as an EPA document. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. All research projects making conclusions or recommendations based on environmentally related measurements and funded by the U.S. Environmental Protection Agency are required to participate in the Agency Quality Assurance Program. This project did not involve environmentally related measurements and, as such, did not require a Quality Assurance Plan. ii Foreword The U.S. Environmental Protection Agency is charged by Congress with protecting the Nation’s land, air, and water resources. Under a mandate of national environmental laws, the Agency strives to formulate and implement actions leading to a compatible balance between human activities and the ability of natural systems to support and nurture life. To meet this mandate, EPA’s research program is providing data
Retrieval of commercials by video semantics (2002)

Videos convey information through several planes of communication, encompassing what is represented in the images how the images are linked together and how the subject is imaged. This feature is stressed in commercials where colors, editing effects, rhythms, and object motion are exploited to influence human purchasing habits. In this paper, based on researches in the marketing field, a link is formalized between low level features of a commercial video and feelings that the video would inspire in the observer. This link is used to define high level indices capturing the main semantics of the video. These indices are embedded in a video retrieval system to support access to a database of video based on their semantics.
Bordetella pertussis adenylate cyclase (1988)

Exposure of Chinese hamster ovary, mouse adrenal cortex tumor (Y-1), THP-1 and U-937 cells and human erythrocytes to adenylate-cyclase-containing urea extracts of Bordetella pertussis (strain 114) organisms promotes the formation of large concentrations of intracellular cAMP. Accumulation is dependent on dose and temperature, with significant accumulation occurring at 4 degrees C, and is virtually instantaneous, with a doubling at 1 min. There is an absolute Ca2+ requirement but external calmodulin (the activator of cyclase activity) has no effect except in erythrocytes and U-937 cells, where it reduces cAMP accumulation. However, calmodulin antagonists inhibit cAMP accumulation. In Y-1 adrenal cells the urea-extract adenylate cyclase stimulates steroidogenesis. Anti-(B. pertussis) antibodies inhibit cyclase activity and prevent further cAMP accumulation after 10 min in cells previously exposed to urea extract. The same effect is obtained by washing. This suggests that a portion of the cyclase is associated with cells in a form not accessible to antibody or washing but accessible to substrate, which we interpret as internalized enzyme with a short lifetime. Continuing cAMP accumulation thus appears to need a continuing source of external cyclase. Inhibitors of the effect of diphtheria toxin, such as NH4Cl, methylamine, chloroquine or monensin, have no inhibitory effect on the accumulation of intracellular cAMP promoted by the internalized adenylate cyclase of urea extracts of B. pertussis organisms. We conclude that entry of the cyclase into cells is not by receptor-mediated endocytosis.
The Impact of Antimicrobial Resistance on Health and Economic Outcomes (2003)

Despite an increasing prevalence of antimicrobial-resistant pathogens, the health and economic impact of colonization and infection with these organisms has not been fully elucidated. We explore how antimicrobial resistance can affect patient outcomes by enhancing virulence, causing a delay in the administration of appropriate therapy, and limiting available therapy. Next, we examine the different perspectives held by hospitals, third-party payers, patients, and society on the impact of resistance. Finally, we review methodological issues in designing and assessing studies that address the clinical outcomes for patients infected or colonized with resistant pathogens, including adjustment for important confounding variables, control group selection, and the quantification of economic outcomes.
Temporal regulation of hyaluronan and proteoglycan metabolism by human bone cells in vitro. (1990)

Osteoblasts elaborate a dynamic extracellular matrix that is constructed and mineralized as bone is formed. This matrix is primarily composed of collagen, along with noncollagenous proteins which include glycoproteins and proteoglycans. After various times in culture, human bone cells were labeled with [35S]sulfate, [3H] leucine/proline, or [3H]glucosamine and the metabolism of hyaluronan and four distinct species of proteoglycans (PGs) was assayed in the medium, cell layer, and intracellular pools. These cells produce hyaluronan (Mr approximately 1,400,000; a chondroitin sulfate PG (CSPG), Mr approximately 600,000; a heparan sulfate PG (HSPG), Mr approximately 400,000; and two dermatan sulfate PGs with Mr approximately 270,000 (biglycan, PG I) and Mr approximately 135,000 (decorin, PG II) that distribute between the medium and cell layer. Two days following subculture, 12 h [35S]sulfate steady-state labeling yielded a composition of 24, 27, 31, and 18% for total CSPG, HSPG, biglycan, and decorin, respectively. While HSPG and decorin levels and distribution between medium and cell layer remained relatively constant during steady-state labeling at different times in culture, CSPG and biglycan levels increased dramatically at late stages of growth, and their distribution changed throughout culture. These results were independent of cell density, media depletion, and labeling pool effects. In contrast, hyaluronan synthesis was uncoupled from PG synthesis and apparently density-dependent. Pulse chase labeling at different stages of culture showed that the CSPG and decorin behaved as secretory PGs. Both HSPG and biglycan underwent catabolism, with HSPG possessing a t1/2 of 8 h and biglycan a t1/2 of 4 h. While the rate of HSPG turnover did not appreciably change between early and late culture, that of biglycan decreased. The mRNA for decorin was constant, while that of biglycan changed during culture. These results suggest that each PG possesses a distinct pattern of cellular and temporal distribution that may reflect specific stages in matrix formation and maturation.
Evaluation of selective encryption techniques for secure transmission of MPEG-compressed bit-streams (2003)

"Total" encryption of MPEG-I compressed bit-streams requires a large processing time. To reduce this processing time, Spanos and Maples (1996) propose encrypting only the data in the Intracoded frames. In this paper, experimental results are presented which indicate that Spanos and Maples' method does not provide an adequate level of security. A better method that calls for encrypting all the Intra-coded macroblocks (I-MB's) in all frames is then presented. Although this method achieves a 69% reduction in the processing time over "total" encryption, the motion content of the video sequence is apparent. Therefore, another method that calls for encrypting the headers of all predicted, macroblocks (MBs) in addition to encrypting the data in all I-MB's is presented. Simulation results for this method show that the decoded video is fully disguised, but at the expense of decreasing the savings in the processing time to 21-60% of the processing time of "total" encryption.
Negative Transconductance Heterojunction Organic Transistors and their Application to Full‐Swing Ternary Circuits (2019)

Multivalued logic (MVL) computing could provide bit density beyond that of Boolean logic. Unlike conventional transistors, heterojunction transistors (H-TRs) exhibit negative transconductance (NTC) regions. Using the NTC characteristics of H-TRs, ternary inverters have recently been demonstrated. However, they have shown incomplete inverter characteristics; the output voltage (V<sub>OUT</sub> ) does not fully swing from V<sub>DD</sub> to G<sub>ND</sub> . A new H-TR device structure that consists of a dinaphtho[2,3-b:2',3'-f]thieno[3,2-b]thiophene (DNTT) layer stacked on a PTCDI-C13 layer is presented. Due to the continuous DNTT layer from source to drain, the proposed device exhibits novel switching behavior: p-type off/p-type subthreshold region /NTC/ p-type on. As a result, it has a very high on/off current ratio (≈10<sup>5</sup> ) and exhibits NTC behavior. It is also demonstrated that an array of 36 of these H-TRs have 100% yield, a uniform on/off current ratio, and uniform NTC characteristics. Furthermore, the proposed ternary inverter exhibits full V<sub>DD</sub> -to-G<sub>ND</sub> swing of V<sub>OUT</sub> with three distinct logic states. The proposed transistors and inverters exhibit hysteresis-free operation due to the use of a hydrophobic gate dielectric and encapsulating layers. Based on this, the transient operation of a ternary inverter circuit is demonstrated for the first time.
Selective Downregulation of JAK2 and JAK3 by an ATP-Competitive pan-JAK Inhibitor (2017)

PF-956980 has been used previously as a JAK3-selective chemical probe in numerous cell-based experiments. Here, we report that not only is PF-956980 a pan-JAK ATP-competitive inhibitor but it also causes selective reduction of endogenous JAK2 and JAK3 protein levels in human primary immune cells (in a time-dependent manner), leaving the other JAK family members (JAK1 and TYK2) unchanged. We found that PF-956980 selectively downregulated JAK2 and JAK3 mRNA, corresponding to changes observed at the protein level. This work highlights therapeutic opportunities for the development of pharmacological inhibitors that also modulate the expression of their cognate binding proteins.
The significance of 3‐D transfer functions in confocal scanning microscopy (1992)

SUMMARY The three‐dimensional (3‐D) transfer function is a useful concept for describing image formation in confocal scanning microscopy. From it we can derive the corresponding 2‐D transfer function for in‐focus imaging. In confocal transmission this can be derived analytically. The 1‐D transfer function for on‐axis imaging, which can be expressed in an analytical form even for confocal fluorescence with differing wavelengths of excitation and fluorescence, can be derived from the 3‐D transfer function. The 2‐D transfer function for in‐focus imaging in confocal fluorescence microscopy with a finite‐sized detector is also presented, which is shown to exhibit sign changes and can therefore result in reversals of image contrast.
Nanopore analysis of nucleic acids (2007)

Thesis (Ph. D.)--University of Washington, 2007.
Efficacy of entomopathogenic fungi against soil‐dwelling life stages of western flower thrips, Frankliniella occidentalis, in plant‐growing media (2008)

Abstract The potential of several entomopathogenic fungi to control soil‐dwelling stages of western flower thrips, Frankliniella occidentalis Pergande (Thysanoptera: Thripidae), was evaluated in various growing media. Two Metarhizium anisopliae (Metsch) Sorokin strains, V275 and ERL700, were the most effective, causing 85–96% mortality of thrips larvae and pupae (as measured by relative adult emergence rates), 11 days after inoculation. Mortality in other M. anisopliae ‐treated media ranged from 51–84%; Beauveria bassiana (Balsamo) Vuillemin strains caused 54–84% mortality, and Paecilomyces fumosoroseus (Wize) 63–75% mortality. In contrast, mortality from the insecticide treatment (fipronil 1 g l −1 medium) was only 15–54%. Metarhizium anisopliae V275 was selected for more in‐depth evaluation under greenhouse conditions. There was no significant difference in M. anisopliae efficacy in growing media whether it was applied as drench (84–93%) or premixed into the medium as dry conidia (85–92%). The use of M. anisopliae with reduced rates of either fipronil or imidacloprid did not significantly improve control. Overall, our study shows that M. anisopliae V275 is robust and offers much promise for the control of soil‐dwelling stages of thrips as part of an integrated pest‐management programme.
Development of Defined Media for the Serum-Free Expansion of Primary Keratinocytes and Human Embryonic Stem Cells (2008)

Primary keratinocyte (Kc) cells and human embryonic stem (hES) cells are routinely propagated on a mouse fibroblast feeder layer in media containing fetal bovine serum or other nondefined factors. One disadvantage of using these nondefined factors is that they may inadvertently contaminate the culture system with infectious agents; thus, there remains a need to develop safe culture conditions free from poorly defined and/or animal products. Our laboratory has discovered that growth factors (GFs) and vitronectin (VN) can bind to each other resulting in synergistic short-term functional effects in several cell types. The aim of the current study was to determine whether primary Kc and hES cells can be established and serially propagated serum-free using medium containing VN, insulin-like growth factor-I, and insulin-like growth factor binding protein-3 (VN:GF). Here we demonstrate that primary Kc cells can be isolated, established, serially propagated, and re-form an epidermal layer using the VN:GF combination. Additionally, cell proliferation studies indicate that the Kcs proliferate using the VN:GF combination at a rate comparable to cells grown using serum. Similarly, we verified that this VN:GF combination could be employed for the serial propagation of hES cells. Importantly, both the Kc and hES cells retain their undifferentiated phenotype when cultured using the VN:GF combinations as a serum-free medium for up to 4 passages for Kc and at least 10 passages for hES cells as indicated by the expression of a range of cell surface markers. This study demonstrates that the novel, fully defined VN:GF medium is a viable alternative to media containing serum and highlights the potential of this technology for generating therapeutically viable cells and tissues.