gebhart/patrepeval huggingface dataset:
P-Selectin Glycoprotein Ligand-1 Is Essential for Adhesion to P-Selectin But Not E-Selectin in Stably Transfected Hematopoietic Cell Lines (1997)

P-selectin (CD62P) is a member of the selectin family of adhesion molecules involved in the regulation of leukocyte traffic. P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like molecule that is thought to be a primary ligand for P-selectin. The interaction of P-selectin with PSGL-1 results in leukocyte rolling and recruitment of leukocytes to sites of inflammation and tissue injury. However, expression of PSGL-1 protein alone is insufficient for binding to P-selectin. Several posttranslational modifications of PSGL-1, including sialylation, sulfation, and fucosylation by alpha 1,3-fucosyltransferase(s) (FucT), are required for functional interaction with P-selectin. Recently, several groups have reported that PSGL-1 might also serve as a ligand for E-selectin. Differential posttranslational modifications of PSGL-1 may determine whether it can interact with either P- or E-selectin or both. To determine whether PSGL-1 is essential for adhesion to P- or E-selectin, we have constructed and analyzed a panel of stably transfected K562 cells. K562 cells express FucT-IV but not FucT-VII or PSGL-1, and do not bind to either E- or P-selectin. K562 cells transfected with PSGL-1 cDNA also did not bind to either P- or E-selectin. Binding to P-selectin occurred only when K562 cells were cotransfected with both FucT-VII and PSGL-1. In contrast, expression of FucT-VII alone was sufficient for E-selectin binding. These data demonstrate that expression of PSGL-1 is not required for adhesion of a stably transfected hematopoietic cell line to E-selectin, and suggest that FucT-IV alone cannot properly modify PSGL-1, expressed in transfected K562 cells, to bind P-selectin.
Nitrogen-doped carbon dots derived from polyvinyl pyrrolidone and their multicolor cell imaging (2014)

Nitrogen-doped carbon dots (N-CDs) with a high quantum yield of 19.6% were prepared by calcining polyvinyl pyrrolidone (PVP, K-30), and then modified with 4,7,10-trioxa-1,13-tridecanediamine. The as-prepared N-CDs exhibited excitation-dependent and pH-sensitive photoluminescence. Transmission electron microscopy and Raman spectra demonstrated the graphitic structure of the N-CDs. Fourier transform infrared spectroscopy and x-ray diffraction studies revealed successful passivation and the presence of hydrophilic groups on the surface. Importantly, such modified quantum dots acted as good multicolor cell imaging probes due to their excellent fluorescent properties, low cytotoxicity and fine dispersity.
High Plasma Levels of MCP-1 and Eotaxin Provide Evidence for an Immunological Basis of Fibromyalgia (2008)

Fibromyalgia (FMS), a predominantly female (85%) syndrome, affects an estimated 2% of the US population with skeletal muscle ache, fatigue, headache, and sleep disorder. The pathogenesis of FMS is unknown and there is no laboratory test for diagnosis. In this study, plasma levels of 25 cytokines and chemokines in 92 female patients with FMS and 69 family members were measured compared to 77 controls. Trans-endothelial migration of normal leukocytes in response to FMS plasma and the cytokine profile of human myoblasts were analyzed. High levels of MCP-1 (P<0.001) and eotaxin (P<0.01) were found in patients and family members compared to controls. Patients (56/92) treated with the single agent guaifenesin (>3 months) had higher levels of eotaxin than those not treated (P<0.01). Diluted plasma from patients increased the migration of normal eosinophils and monocytes, but not neutrophils, through an endothelial/Matrigel barrier only when mast cells are included in the lower wells (P<0.05). Furthermore, myoblasts can secrete MCP-1, eotaxin, and IP-10, while treatment with MCP-1 caused secretion of IL-1beta, eotaxin and IP-10. FMS is associated with inflammatory chemokines, that MCP-1 and eotaxin may contribute to the symptoms of FMS, and that similar cytokine profiles found in family members support the idea that FMS has a genetic component. Furthermore, the chemokine profile associated with FMS has direct effects on the migration of eosinophils and monocytes in the presence of mast cells, and skeletal muscle itself may secrete.
The surgical management of the refractory overactive bladder (2010)

The refractory overactive bladder is a clinically challenging entity to manage and affects millions of people worldwide. Current surgical treatment options include botulinum toxin type A, sacral neuromodulation, and bladder reconstruction surgery all of which require careful attention to the individual patients needs and circumstances. In our paper we present a detailed up-to-date review on all the above mentioned surgical techniques from current literature and briefly describe our units experience with sacral neuromodulation.
T-DNA Binary Vectors and Systems (2008)

For more than two decades, scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants. Initial technologies to introduce genes of interest (goi) into Agrobacterium involved complex microbial genetic methodologies that inserted these goi into the transfer DNA (T-DNA) region of large tumor-inducing plasmids (Ti-plasmids). However, scientists eventually learned that T-DNA transfer could still be effected if the T-DNA region and the virulence (vir) genes required for T-DNA processing and transfer were split into two replicons. This binary system permitted facile manipulation of Agrobacterium and opened up the field of plant genetic engineering to numerous laboratories. In this review, we recount the history of development of T-DNA binary vector systems, and we describe important components of these systems. Some of these considerations were previously described in a review by Hellens et al. (2000b).
Engineering Camel Single-Domain Antibodies and Immobilization Chemistry for Human Prostate-Specific Antigen Sensing (2005)

The specificity and affinity characteristics of antibodies make them excellent probes in biosensor applications. Unfortunately, their large size, unstable behavior, and random immobilization properties create numerous problems. The single-domain antigen-binding fragment derived from heavy-chain antibodies of camelids (termed VHH) offers special advantages in terms of size, stability, and ease of generating different antibody constructs. In this study, we show the potential of those VHHs in sensing human prostate-specific antigen (hPSA) by SPR technology. Different VHH constructs were immobilized onto commercial and custom-built sensor surfaces by metal chelation, biotin-streptavidin interaction, or covalent coupling. The detection of subnanogram per milliliter hPSA concentrations could be attained on a covalently coupled three-dimensional dextran surface. Moreover, the ratio of different hPSA isoform concentrations could be assessed via a sandwich assay and resulted in the detection of clinically significant antigen concentrations within 15 min. In addition, for the first time, the intrinsic protein stability is presented as an important probe design factor, since our results reveal that higher intrinsic stability offers higher resistance to harsh regeneration conditions. In conclusion, we present VHHs as a novel class of biosensor probes rivaling conventional antibodies and their derived antibody fragments.
Circulating MYCN DNA as a tumor-specific marker in neuroblastoma patients. (2002)

MYCN oncogene amplification is an established indicator of the aggressiveness of neuroblastomas; it is used internationally for stratifying patients for therapy. The present study shows that high levels of MYCN DNA sequences are present in the peripheral blood of patients with MYCN-amplified neuroblastomas. Circulating MYCN DNA may be a powerful and noninvasive prognostic marker at the time of diagnosis. Furthermore, preliminary data strongly suggest that the release of MYCN sequences in the peripheral blood is an early process in disease progression, permitting us to propose this novel marker for the follow-up of patients after chemotherapy.
CSF corticotropin-releasing factor-like immunoreactivity in depression and schizophrenia (1987)

To further investigate the hypothesis that hyperactivity of the hypothalamic-pituitary-adrenal axis in patients with depression may be mediated by hypersecretion of corticotropin-releasing factor (CRF), the authors measured CRF-like immunoreactivity in CSF samples from 138 neurological control, 54 depressed, and 27 nondepressed (23 schizophrenic and four manic) subjects. The CSF CRF concentration was markedly higher (almost twofold) in depressed patients than in control subjects and nondepressed psychiatric patients. The concentration of CSF CRF was slightly but significantly higher in schizophrenic patients than in control subjects. These findings provide further support for the hypothesis that CRF hypersecretion occurs in major depression.
Metabolism of Pyruvate by Epididymal-Like Bovine Spermatozoa (1964)

Various factors affecting aerobic and anaerobic pyruvate metabolism by bovine spermatozoa were studied. With pyruvate as the sole exogenous substrate, DNP (10' ~) stimulated the respiration of ELC (epididymal-like sperm cells) of some bulls but had no effect on the respiration of others. DNP also depressed the aerobic pyruvate utilization and lactate accumulation by ELC. The dismutation reaction, in which two molecules of pyruvate undergo dismutatlon to one molecule each of lactate, CO~, and acetate, was studied and was found to be stimulated anaerobically by both phosphate and sulfite. Phosphate, NAD, lipoic acid, and ATP stimulated the respiratory activity of ELC in the presence of pyruvate.
Smallpox as a Biological Weapon (1999)

An aerosolized plague weapon could cause fever, cough, chest pain, and hemoptysis with signs consistent with severe pneumonia 1 to 6 days after exposure. Rapid evolution of disease would occur in the 2 to 4 days after symptom onset and would lead to septic shock with high mortality without early treatment. Early treatment and prophylaxis with streptomycin or gentamicin or the tetracycline or fluoroquinolone classes of antimicrobials would be advised.
Reactions of Nitrogen(II) Oxide (1962)

The structure, properties, and familiar reactions of nitrogen(II) oxide are reviewed briefly. The discussion is mainly concerned with reactions of basic molecules with NO that give rise to products containing the N2O2 group. The reactions of nitric oxide with sulfite ion, ethyl alcohol, amines, and oximes are discussed. These reactions are correlated through a generalized reaction scheme that involves formation of the reactive free radical intermediate BNO, where B is a base. In addition, reactions that involve base attack on nitric oxide but lead to other products are reviewed. These include nitric oxide reactions with phosphorus and sulfur donors and with organic nitrites.
Lysophospholipid Acyltransferases and Arachidonate Recycling in Human Neutrophils (2008)

The cycle of deacylation and reacylation of phospholipids plays a critical role in regulating availability of arachidonic acid for eicosanoid production. The major yeast lysophospholipid acyltransferase, Ale1p, is related to mammalian membrane-bound O-acyltransferase (MBOAT) proteins. We expressed four human MBOATs in yeast strains lacking Ale1p and studied their acyl-CoA and lysophospholipid specificities using novel mass spectrometry-based enzyme assays. MBOAT1 is a lysophosphatidylserine (lyso-PS) acyltransferase with preference for oleoyl-CoA. MBOAT2 also prefers oleoyl-CoA, using lysophosphatidic acid and lysophosphatidylethanolamine as acyl acceptors. MBOAT5 prefers lysophosphatidylcholine and lyso-PS to incorporate linoleoyl and arachidonoyl chains. MBOAT7 is a lysophosphatidylinositol acyltransferase with remarkable specificity for arachidonoyl-CoA. MBOAT5 and MBOAT7 are particularly susceptible to inhibition by thimerosal. Human neutrophils express mRNA for these four enzymes, and neutrophil microsomes incorporate arachidonoyl chains into phosphatidylinositol, phosphatidylcholine, PS, and phosphatidylethanolamine in a thimerosal-sensitive manner. These results strongly implicate MBOAT5 and MBOAT7 in arachidonate recycling, thus regulating free arachidonic acid levels and leukotriene synthesis in neutrophils.
30Gbit/s PAM-4 transmission by modulating a dual silicon ring resonator modulator (2016)

PAM-4 transmission is established by modulating two silicon ring modulators in series with two uncorrelated NRZ encoded data sources. The two modulators are thermally tuned to obtain different extinction ratios. 30Gb/s PAM-4 transmissions are achieved with only 1Vpp.
Neural networks for control (1999)

Provides a quick overview of neural networks and explains how they can be used in control systems. We introduce the multilayer perceptron neural network and describe how it can be used for function approximation. The backpropagation algorithm (including its variations) is the principal procedure for training multilayer perceptrons; it is briefly described here. Care must be taken, when training perceptron networks, to ensure that they do not overfit the training data and then fail to generalize well in new situations. Several techniques for improving generalization are discussed. The article also presents several control architectures, such as model reference adaptive control, model predictive control, and internal model control, in which multilayer perceptron neural networks can be used as basic building blocks.
The Lipid Storage Diseases: New Concepts and Control (1975)

The nature of the enzymatic defect is now well established in ten inherited disorders of lipid metabolism. This information has provided for the development of facile, sensitive tests using readily available materials such as washed leukocytes or cultured skin fibroblasts for the diagnosis of these disorders; the detection of heterozygous carriers of these traits; and the monitoring of pregnancies at risk for any of these conditions. Recent investigations of enzyme replacement therapy have shown great promise for the treatment of patients with Fabry's disease and those with the adult form of Gaucher's disease. Additional procedures must be developed for successful enzyme replacement in patients where the central nervous system is damaged by the accumulation of lipids.
Analyzing the potato abiotic stress transcriptome using expressed sequence tags (2005)

To further increase our understanding of responses in potato to abiotic stress and the potato transcriptome in general, we generated 20 756 expressed sequence tags (ESTs) from a cDNA library constructed by pooling mRNA from heat-, cold-, salt-, and drought-stressed potato leaves and roots. These ESTs were clustered and assembled into a collection of 5240 unique sequences with 3344 contigs and 1896 singleton ESTs. Assignment of gene ontology terms (GOSlim/Plant) to the sequences revealed that 8101 assignments could be made with a total of 3863 molecular function assignments. Alignment to a set of 78 825 ESTs from other potato cDNA libraries derived from root, leaf, stolon, tuber, germinating eye, and callus tissues revealed 1476 sequences unique to abiotic stressed potato leaf and root tissue. Sequences present within the 5240 sequence set had similarity to genes known to be involved in abiotic stress responses in other plant species such as transcription factors, stress response genes, and signal transduction processes. In addition, we identified a number of genes unique to the abiotic stress library with unknown function, providing new candidate genes for investigation of abiotic stress responses in potato.
Deconvolutional networks (2010)

Building robust low and mid-level image representations, beyond edge primitives, is a long-standing goal in vision. Many existing feature detectors spatially pool edge information which destroys cues such as edge intersections, parallelism and symmetry. We present a learning framework where features that capture these mid-level cues spontaneously emerge from image data. Our approach is based on the convolutional decomposition of images under a spar-sity constraint and is totally unsupervised. By building a hierarchy of such decompositions we can learn rich feature sets that are a robust image representation for both the analysis and synthesis of images.
Learning over sets using kernel principal angles (2003)

We consider the problem of learning with instances defined over a space of sets of vectors. We derive a new positive definite kernel f (A,B) defined over pairs of matrices A,B based on the concept of principal angles between two linear subspaces. We show that the principal angles can be recovered using only inner-products between pairs of column vectors of the input matrices thereby allowing the original column vectors of A,B to be mapped onto arbitrarily high-dimensional feature spaces. We demonstrate the usage of the matrix-based kernel function f (A,B) with experiments on two visual tasks. The first task is the discrimination of “irregular ” motion trajectory of an individual or a group of individuals in a video sequence. We use the SVM approach using f (A,B) where an input matrix represents the motion trajectory of a group of individuals over a certain (fixed) time frame. We show that the classification (irregular versus regular) greatly outperforms the conventional representation where all the trajectories form a single vector. The second application is the visual recognition of faces from input video sequences representing head motion and facial expressions where f (A,B) is used to compare two image sequences.
Retinal nerve fiber layer thickness map determined from optical coherence tomography images (2005)

We introduce a method to determine the retinal nerve fiber layer (RNFL) thickness in OCT images based on anisotropic noise suppression and deformable splines. Spectral-Domain Optical Coherence Tomography (SDOCT) data was acquired at 29 kHz A-line rate with a depth resolution of 2.6 mum and a depth range of 1.6 mm. Areas of 9.6x6.4 mm2 and 6.4x6.4 mm2 were acquired in approximately 6 seconds. The deformable spline algorithm determined the vitreous-RNFL and RNFL-ganglion cell/inner plexiform layer boundary, respectively, based on changes in the reflectivity, resulting in a quantitative estimation of the RNFL thickness. The thickness map was combined with an integrated reflectance map of the retina and a typical OCT movie to facilitate clinical interpretation of the OCT data. Large area maps of RNFL thickness will permit better longitudinal evaluation of RNFL thinning in glaucoma.
VEGF-C–induced lymphangiogenesis in sentinel lymph nodes promotes tumor metastasis to distant sites (2006)

The mechanisms by which tumors metastasize to sentinel and distant lymph nodes, and beyond, are poorly understood. We developed transgenic mice that overexpress vascular endothelial growth factor-C (VEGF-C) and green fluorescent protein specifically in the skin and studied the effects of chemically-induced skin carcinogenesis in this model. We found that in contrast to VEGF-A, VEGF-C does not increase the growth of primary tumors, but instead induces expansion of lymphatic networks within sentinel lymph nodes, even before the onset of metastasis. Once the metastatic cells arrived at the sentinel lymph nodes, the extent of lymphangiogenesis at these sites increased. Of importance, in mice with metastasis-containing sentinel lymph nodes, tumors that expressed VEGF-C were more likely to metastasize to additional organs, such as distal lymph nodes and lungs. No metastases were observed in distant organs in the absence of lymph node metastases. These findings indicate an important role of VEGF-C-induced lymph node lymphangiogenesis in the promotion of cancer metastasis beyond the sentinel lymph nodes. VEGF-C is therefore a good target to slow or even prevent the onset of metastasis.
Organ-associated macrophage precursor activity: isolation of candidacidal and tumoricidal effectors from the spleens of cyclophosphamide-treated mice. (1986)

We recently reported the modulating effects of a single injection of the anti-neoplastic drug cyclophosphamide (Cy; 150 mg/kg i.p.) on in vivo resistance against the experimental Candida albicans infection. Increased resistance to microbial challenge occurred 12 to 18 days after treatment. We now show that the increased resistance is paralleled by the appearance of potent nonadherent nonphagocytic effectors in the spleen (day 12) that are capable both of candidacidal activity and natural killer (NK) activity against YAC-1 cells. The cells mediating the two reactivities have a low buoyant density, a strong proliferating activity in response to the macrophage colony stimulating factor (CSF-1), and are unable to kill the NK-insensitive lines EL-4 and P815. A clear cut isolation of macrophage precursor cells from this Percoll low density fraction has been performed in an indirect rosette assay on the basis of their positivity for the surface markers recognized by the highly specific rat-anti-mouse macrophages, monoclonal antibodies M143 and F4/80. We obtained an extremely homogeneous population of cells in the early stage of macrophage differentiation that is responsible for all the candidacidal activity and for a major part of the NK activity observed in the spleen of Cy-treated mice, and which is extremely sensitive to CSF-1 induction.
Propagation model for the HVAC duct as a communication channel (2003)

Heating, ventilation, and air conditioning (HVAC) ducts in buildings are typically hollow metal pipes which can be used as waveguides to carry signals and provide network access to offices. Knowledge of channel properties is crucial to designing such a communication system. The paper presents a propagation model for a straight HVAC duct terminated at both ends. At high frequencies, this duct behaves as a multimode waveguide with a transmitting antenna coupling in and a receiving antenna coupling out. We derive a simple analytical expression for the frequency response of this channel using conventional techniques. Experimental data taken on real circular ducts excited by monopole probe antennas confirm the theoretical results. This model represents an initial step toward the development of a tool for planning a wireless distribution system using building HVAC ducts.
Combined shear force and near-field scanning optical microscopy (1992)

A distance regulation method has been developed to enhance the reliability, versatility, and ease of use of near-field scanning optical microscopy (NSOM). The method relies on the detection of shear forces between the end of a near-field probe and the sample of interest. The system can be used solely for distance regulation in NSOM, for simultaneous shear force and near-field imaging, or for shear force microscopy alone. In the latter case, uncoated optical fiber probes are found to yield images with consistently high resolution.
Specific Language Impairment: A Review of Current Trends and Issues for Future Research (2014)

特異的言語障害の症状の最も顕著な特徴は、発話時にみられる屈折接辞や機能語などの文法形態素の脱落である。この文法障害の発現機構はまだ解明されておらず、さまざまな仮説が提唱されている。本稿では、これらの仮説の中で音韻処理障害仮説に属する2つの説を検証し、その妥当性について検討した。具体的には、1）急速な音の変化を伴う音の弁別過程の問題、2）音韻ワーキングメモリ・音韻短期記憶の能力の低下、で文法障害の諸症状を論理的に説明することができるのか検証した。今後の課題としては、横断的な研究をする場合には、言語特徴の異なるサブタイプの存在に十分に留意して適切に対象児を選択する、縦断的な研究をする場合には、対象児の年齢層に留意する、ことが重要であると指摘した。また、日本語において特異的言語障害の文法障害の言語特徴を明らかにするにあたって、言語学的な視点から今後の研究の方向性について提言をするとともに研究手法上の留意する点を指摘した。