gebhart/patrepeval huggingface dataset:
Influences of the lysosomal associated membrane proteins (Lamp‐1, Lamp‐2) and Mac‐2 binding protein (Mac‐2‐BP) on the prognosis of pancreatic carcinoma (2001)

Lamp-1 might influence local tumor progression rather than the formation of tumor metastasis in pancreatic carcinoma, whereas Mac-2-BP and Lamp-2 seem to have little influence on these parameters in pancreatic carcinoma.
The Low-Density Lipoprotein Pathway and its Relation to Atherosclerosis (1977)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Serial analysis of gene expression identifies putative metastasis-associated transcripts in colon tumour cell lines (2000)

We have used serial analysis of gene expression (SAGE) to identify gene expression differences between a primary colon tumour cell line (SW480) and an isogenic lymph-node metastasis cell line (SW620). Differential expression was confirmed for the following genes: keratin K5, cystatin S, serum amyloid A, the human homologue of yeast ribosomal S28 and the p32 subunit of human pre-mRNA splicing factor SF2. Expression of confirmed differences were also analysed in other metastatic cell lines.
Post-CMOS Processing for High-Aspect-Ratio (2002)

We present a new fabrication sequence for inte- grated-silicon microstructures designed and manufactured in a conventional complentary metal-oxide-semiconductor (CMOS) process. The sequence employs a post-CMOS deep silicon back- side etch, which allows fabrication of high aspect ratio (25:1) and flat (greater than 10 mm radius of curvature) MEMS devices with integrated circuitry. A comb-drive resonator, a cantilever beam array and a -axis accelerometer were fabricated using this process sequence. Electrical isolation of single-crystal silicon was realized by using the undercut of the reactive ion etch (RIE) process. Measured out-of-plane curling across a 120- m-wide 25- m-thick silicon released plate was 0.15 m, which is about ten times smaller than curl of the identical design as a thin-film CMOS microstructure. The -axis DRIE accelerometer structure is 0.4 mm by 0.5 mm in size and has a 25- m-thick single-crystal silicon proof mass. The measured noise floor is 1 mG/ Hz, limited by electronic noise. A vertical electrostatic spring hardening effect was theoretically predicted and experimentally verified. (687)
Electrophilic aromatttic substitution reactions by platinum(II) and palladium(II) chlorides on N,N-dimethylbenzylamines (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrophilic aromatttic substitution reactions by platinum(II) and palladium(II) chlorides on N,N-dimethylbenzylaminesArthur C. Cope and Edwin C. FriedrichCite this: J. Am. Chem. Soc. 1968, 90, 4, 909–913Publication Date (Print):February 14, 1968Publication History Published online1 May 2002Published inissue 14 February 1968https://pubs.acs.org/doi/10.1021/ja01006a012https://doi.org/10.1021/ja01006a012research-articleACS PublicationsRequest reuse permissionsArticle Views2332Altmetric-Citations463LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Lesioni recenti della colonna toracolombare (2005)

Riassunto Nell’esperienza degli Autori (escludendo lesioni vertebrali minori che possono essere trattate funzionalmente), circa il 60% delle lesioni piu severe puo essere trattato con terapia conservativa, e solo il 30% orca richiede un intervento.
Epigenetic analysis of childhood acute lymphoblastic leukemia (2009)

We used a chromosome 3 wide NotI microarray for identification of epigenetically inactivated genes in childhood acute lymphoblastic leukemia (ALL). Three novel genes demonstrated frequent methylation in childhood ALL. PPP2R3A (protein phosphatase 2, regulatory subunit B", alpha) was frequently methylated in T (69%) and B (82%)-ALL. Whilst FBLN2 (fibulin 2) and THRB (thyroid hormone receptor, beta) showed frequent methylation in B-ALL (58%; 56% respectively), but were less frequently methylated in T-ALL (17% for both genes). Recently it was demonstrated that BNC1 (Basonuclin 1) and MSX1 (msh homeobox 1) were frequently methylated across common epithelial cancers. In our series of childhood ALL BNC1 was frequently methylated in both T (77%) and B-ALL (79%), whilst MSX1 showed T-ALL (25%) specific methylation. The methylation of the above five genes was cancer specific and expression of the genes could be restored in methylated leukemia cell lines treated with 5-aza-2'-deoxycytidine. This is the first report demonstrating frequent epigenetic inactivation of PPP2R3A, FBLN2, THRB, BNC1 and MSX1 in leukemia. The identification of frequently methylated genes showing cancer specific methylation will be useful in developing early cancer detection screens and for targeted epigenetic therapies.
Amphiphilic Phenyl−Ethynylene Polymers and Copolymers. Synthesis, Characterization, and Optical Emission Properties (2003)

A series of new rigid amphiphilic aryl−ethynylene homopolymers and copolymers with phenyl, pyridinyl, thionyl, or carbazolyl as aryl units has been synthesized. A single side chain on the phenyl rings (alkyl−hydroxyl ester group) provides an amphiphilic character to the polymer backbone. The polymers are characterized by NMR, elemental analysis and size exclusion chromatography (SEC); the thermal properties are analyzed by TGA, DSC, and X-ray diffraction. The polymers with side chains are enough amphiphilic to give stable Langmuir films on water, and the LB films can be transferred on hydrophilic or hydrophobic substrates. The films are deposited on treated glass or silicon substrate. Analyzed by grazing incidence X-ray analysis (GIXA) and by atomic force microscopy (AFM), they reveal well-structured layering periods of 3.5 and 2.7−2.9 nm for the phenyl homopolymer (pPEnBz) and the copolymers with pyridine (pPEn(Bz-co-Py)) and thiophene (pPEn(Bz-co-Ti)), respectively. These results suggest a rearrangement in a Y-type bilayer for the pPEnBz on a hydrophilic substrate. For copolymers pPEn(Bz-co-Py), and pPEn(Bz-co-Ti), deposited on a hydrophobic substrate, the self-assembly within one layer consists of interdigitated alkyl chains. In solid-state films the polymers and copolymers show a rather good photoluminescence yield and large Stokes-shifts with emission peaks at 539, 502, 569, and 563 nm for pPEnBz, pPEn(Bz-co-Py), pPEn(Bz-co-Ti), and pPEn(Bz-co-Cz), respectively. The photoluminescence quantum yields are decreasing in the series by 23, 15, 12, and 7% respectively, while no emission is observed from pPEnPy. A dichroism on LB films has been found in absorption (R = 2.0, 2.04 and 1.3) and in emission spectra (R = 3.4, 4.0 and 2.6) for pPEnBz, pPEn(Bz-co-Ti) and pPEn(Bz-co-Py), respectively. LED properties are demonstrated using the ITO/polymer/LiF−Al sandwich, yielding photon emission at 555 and 550 nm for pPEnBz and pPEn(Bz-co-Py). These results are very important for the future realization by LB deposition technique of more sophisticated devices for polarized emission with further enhanced brightness and improved light efficiency.
Electrical and Structural Properties of Ti/Au Ohmic Contacts to n-ZnO (2001)

We have investigated Ti/Au (30/50 nm) ohmic contacts to The samples are annealed at temperatures of 300 and 500°C for 60 s in a flowing atmosphere. Current-voltage measurements show that the as-deposited sample is ohmic with a specific contact resistance of However, annealing of the sample at 300°C results in much better ohmic behavior with a contact resistance of Further increase in annealing temperature (500°C) causes the degradation of the ohmic property. Glancing angle X-ray diffraction and Auger electron spectroscopy are used to investigate interfacial reactions between the Ti/Au and ZnO layers. It is shown that both rutile and srilankite phases are formed in the as-deposited and annealed samples. It is further shown that annealing at 500°C results in the formation of new phases such as and A possible explanation is given to describe the annealing temperature dependence of the specific contact resistance. © 2001 The Electrochemical Society. All rights reserved.
Amelioration of progressive renal injury by genetic manipulation of Klotho gene (2007)

Klotho, an antiaging gene with restricted organ distribution, is mainly expressed in the kidney tubules; the mutant mice have shortened life span, arteriosclerosis, anemia, and osteoporesis, features common to patients with chronic renal failure. Conceivably, the reduction of the Klotho gene expression may contribute to the development of kidney failure; alternatively, its overexpression may lead to the amelioration of renal injury in an ICR-derived glomerulonephritis (ICGN) mouse model with subtle immune complex-mediated disease. To address this issue, four different strains of mice were generated by cross-breeding: ICGN mice without the Klotho transgene (ICGN), ICGN mice with the Klotho transgene (ICGN/klTG), wild-type mice with the Klotho transgene (klTG), and wild-type mice without the Klotho transgene (control). At 40 weeks old, the survival rate was approximately 30% in ICGN mice, and approximately 70% in the ICGN/klTG group. This improvement was associated with dramatic improvement in renal functions, morphological lesions, and cytochrome c oxidase activity but a reduction in beta-galactosidase activity (a senescence-associated protein), mitochondrial DNA fragmentation, superoxide anion generation, lipid peroxidation, and Bax protein expression and apoptosis. Interestingly, improvement was seen in both the tubular and glomerular compartments of the kidney, although Klotho is exclusively confined to the tubules, suggesting that its gene product has a remarkable renoprotective effect by potentially serving as a circulating hormone while mitigating the mitochondrial oxidative stress.
Molecular and Biochemical Characterization of the ADP-dependent Phosphofructokinase from the Hyperthermophilic Archaeon Pyrococcus furiosus (1999)

Pyrococcus furiosus uses a modified Embden-Meyerhof pathway involving two ADP-dependent kinases. Using the N-terminal amino acid sequence of the previously purified ADP-dependent glucokinase, the corresponding gene as well as a related open reading frame were detected in the genome of P. furiosus. Both genes were successfully cloned and expressed in Escherichia coli, yielding highly thermoactive ADP-dependent glucokinase and phosphofructokinase. The deduced amino acid sequences of both kinases were 21.1% identical but did not reveal significant homology with those of other known sugar kinases. The ADP-dependent phosphofructokinase was purified and characterized. The oxygen-stable protein had a native molecular mass of approximately 180 kDa and was composed of four identical 52-kDa subunits. It had a specific activity of 88 units/mg at 50 degrees C and a pH optimum of 6.5. As phosphoryl group donor, ADP could be replaced by GDP, ATP, and GTP to a limited extent. The K(m) values for fructose 6-phosphate and ADP were 2.3 and 0.11 mM, respectively. The phosphofructokinase did not catalyze the reverse reaction, nor was it regulated by any of the known allosteric modulators of ATP-dependent phosphofructokinases. ATP and AMP were identified as competitive inhibitors of the phosphofructokinase, raising the K(m) for ADP to 0.34 and 0.41 mM, respectively.
Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry (2007)

Ubiquitination plays an essential role in maintaining cellular homeostasis by regulating a multitude of essential processes. The ability to identify ubiquitinated proteins by MS currently relies on a strategy in which ubiquitinated peptides are identified by a 114.1 Da diglycine (GG) tag on lysine residues, which is derived from the C-terminus of ubiquitin, following trypsin digestion. In the following study, we report a more comprehensive approach for mapping ubiquitination sites by trypsin digestion and MS/MS analysis. We demonstrate that ubiquitination sites can be identified by signature peptides containing a GG-tag (114.1 Da) and an LRGG-tag (383.2 Da) on internal lysine residues as well as a GG-tag found on the C-terminus of ubiquitinated peptides. Application of this MS-based approach enabled the identification of 96 ubiquitination sites from proteins purified from human MCF-7 breast cancer cells, representing a 2.4-fold increase in the number of ubiquitination sites that could be identified over standard methods. Our improved MS-based strategy will aid future studies which aim to identify and/or characterize ubiquitinated proteins in human cells.
Antimicrobial properties of liposomal azithromycin for Pseudomonas infections in cystic fibrosis patients (2014)

The results of this research indicate that this novel liposomal azithromycin formulation could be a useful therapy to enhance the safety and efficacy of azithromycin against P. aeruginosa-infected persons.
Quantitative Detection of Borrelia burgdorferi in 2-Millimeter Skin Samples of Erythema Migrans Lesions: Correlation of Results with Clinical and Laboratory Findings (2002)

Variability of disease manifestations has been noted in patients with Lyme disease. A contributing factor to this variation may be the number of spirochetes present in infected patients. We evaluated clinical and laboratory findings for patients with erythema migrans with regard to the number of Borrelia burgdorferi organisms detected by quantitative PCR (qPCR) in 2-mm skin biopsy specimens. B. burgdorferi was detected in 80% (40 of 50) of the specimens tested; the mean number of spirochetes in these specimens ranged over 3 orders of magnitude (10 to 11,000 spirochetes per 2-mm biopsy specimen). Larger numbers of spirochetes were significantly associated with a shorter duration of the erythema migrans skin lesion (P = 0.020), smaller skin lesions (P = 0.020), and infection with a specific genotype of B. burgdorferi (P = 0.008) but not with the number or severity of symptoms. Skin culture positivity was significantly associated with skin lesions containing larger numbers of spirochetes (P = 0.019).
Towards Automated Assessment of Public Speaking Skills Using Multimodal Cues (2014)

Traditional assessments of public speaking skills rely on human scoring. We report an initial study on the development of an automated scoring model for public speaking performances using multimodal technologies. Task design, rubric development, and human rating were conducted according to standards in educational assessment. An initial corpus of 17 speakers with 4 speaking tasks was collected using audio, video, and 3D motion capturing devices. A scoring model based on basic features in the speech content, speech delivery, and hand, body, and head movements significantly predicts human rating, suggesting the feasibility of using multimodal technologies in the assessment of public speaking skills.
Zinc-Finger Nuclease Based Genome Surgery: Its All About Specificity (2011)

Therapeutic genome engineering is a hallmark of gene therapy but only recent technological advances have permitted the modification of complex genomes in a targeted fashion. Zinc-finger nucleases (ZFNs) have developed into a major playmaker in the genome engineering field and have been employed to trigger the targeted editing of genomes at over 50 gene loci in 11 model organisms, including fruitfly, zebrafish and rat, with allelic frequencies reaching the double digit percentage range. Moreover, ZFN-mediated genome surgery in primary human cells has become a reality and two phase I clinical trials aiming at knocking out the CCR5 receptor in T cells isolated from HIV patients to protect these cells from infection with the virus have been initiated. Considering that specificity is closely linked to ZFN activity and ZFN-associated toxicity, this parameter has been and will be a key quality in any therapeutic application of the designer nucleases. This review summarizes the technological innovations that have successfully catapulted ZFNs into the genome engineering arena and provides an overview of the current state of the art of these nucleases with reference to human gene therapy.
Trehalose Is a Potent PCR Enhancer: Lowering of DNA Melting Temperature and Thermal Stabilization of Taq Polymerase by the Disaccharide Trehalose (2004)

patients with severe hemolytic jaundice and high copper, but many countries have limited use of chelation therapy to late childhood and adulthood. -Tocopherol or other antioxidants may be administrated as usual (25 ).
Near Infrared Photoelasticity of Polycrystalline Silicon and it's Relation to In-Plane Residual Stresses (2005)

The goal of this research was to investigate an experimental infrared transmission technique and associated analysis tools that extract the in-plane residual stresses in thin single and poly-crystalline silicon sheet, and try to relate the residual stresses to physical parameters associated with silicon growth and cell processing.\n\nPrevious research has suggested this concept, but many engineering and analytical details had not been addressed. In this research, a system has been designed and built. A fringe multiplier was incorporated into the system to increase the sensitivity. The error was analyzed and the resolution of the system was found to be 1.2~MPa. To convert the experimental results to residual stresses, the stress-optic coefficients of (001), (011) and (111) silicon were analyzed analytically and calibrated using a four-point bending fixture. Anisotropy in (001) and (011) silicon was found to be 33%, and the coefficient of EFG silicon is 1.7 times larger than that of (001) silicon.\n\nThe polariscope together with other techniques was applied to silicon wafers after various\nprocessing steps in the manufacture of photovoltaic cells. The influence of the processing on residual stress was investigated and positive correlations between residual stresses, PL and efficiency were obtained.
Current management of massive hemorrhage in trauma (2012)

Hemorrhage remains a major cause of potentially preventable deaths. Trauma and massive transfusion are associated with coagulopathy secondary to tissue injury, hypoperfusion, dilution, and consumption of clotting factors and platelets. Concepts of damage control surgery have evolved prioritizing early control of the cause of bleeding by non-definitive means, while hemostatic control resuscitation seeks early control of coagulopathy.Hemostatic resuscitation provides transfusions with plasma and platelets in addition to red blood cells in an immediate and sustained manner as part of the transfusion protocol for massively bleeding patients. Although early and effective reversal of coagulopathy is documented, the most effective means of preventing coagulopathy of massive transfusion remains debated and randomized controlled studies are lacking. Viscoelastical whole blood assays, like TEG and ROTEM however appear advantageous for identifying coagulopathy in patients with severe hemorrhage as opposed the conventional coagulation assays.In our view, patients with uncontrolled bleeding, regardless of it's cause, should be treated with hemostatic control resuscitation involving early administration of plasma and platelets and earliest possible goal-directed, based on the results of TEG/ROTEM analysis. The aim of the goal-directed therapy should be to maintain a normal hemostatic competence until surgical hemostasis is achieved, as this appears to be associated with reduced mortality.
Genetic Mapping of Quantitative Trait Loci for Growth and Fatness in Pigs (1994)

The European wild boar was crossed with the domesticated Large White pig to genetically dissect phenotypic differences between these populations for growth and fat deposition. The most important effects were clustered on chromosome 4, with a single region accounting for a large part of the breed difference in growth rate, fatness, and length of the small intestine. The study is an advance in genome analyses and documents the usefulness of crosses between divergent outbred populations for the detection and characterization of quantitative trait loci. The genetic mapping of a major locus for fat deposition in the pig could have implications for understanding human obesity.
ZenSheet studio: a spreadsheet-inspired environment for reactive computing (2017)

Widely criticized as error-prone, causing millions of dollars in losses, spreadsheets continue to be extremely popular, decades after their introduction. The ZenSheet project aims to turn spreadsheets into modern and robust computing environments, with minimal impact on the learning curve. This paper focuses on ZenSheet Studio: a web client that delivers a generalized spreadsheet computing experience.
Ex Vivo Expansion of Hematopoietic Stem Cells for Therapeutic Purposes: Lessons from Development and the Niche (2019)

Expansion of hematopoietic stem cells (HSCs) for therapeutic purposes has been a "holy grail" in the field for many years. Ex vivo expansion of HSCs can help to overcome material shortage for transplantation purposes and genetic modification protocols. In this review, we summarize improved understanding in blood development, the effect of niche and conservative signaling pathways on HSCs in mice and humans, and also advances in ex vivo culturing protocols of human HSCs with cytokines or small molecule compounds. Different expansion protocols have been tested in clinical trials. However, an optimal condition for ex vivo expansion of human HSCs still has not been found yet. Translating and implementing new findings from basic research (for instance by using genetic modification of human HSCs) into clinical protocols is crucial to improve ex vivo expansion and eventually boost stem cell gene therapy.
Precursor Frequency and Competition Dictate the HLA-A2–Restricted CD8+ T Cell Responses to Influenza A Infection and Vaccination in HLA-A2.1 Transgenic Mice (2011)

The human HLA-A2-restricted CD8(+) T cell response to influenza A virus (IAV) is largely directed against the matrix protein-derived M1(58-66) epitope and represents an archetypal example of CD8(+) T cell immunodominance. In this study, we examined the CD8(+) T cell hierarchy to M1(58-66) and two subdominant IAV-specific epitopes: NS1(122-130) and PA(46-55) in HLA-A2(+) human subjects and HLA-A2.1 transgenic (HHD) mice. Using epitope-based lipopeptides, we show that the CD8(+) T cell hierarchy induced by IAV infection could also be induced by lipopeptide vaccination in a context outside of viral infection when the Ag load is equalized. In the HHD HLA-A2.1 mouse model, we show that the naive T cell precursor frequencies, and competition at the Ag presentation level, can predict the IAV-specific CD8(+) T cell hierarchy. Immunization of mice with subdominant epitopes alone was unable to overcome the dominance of the M1(58-66)-specific response in the face of IAV challenge; however, a multiepitope vaccination strategy was most effective at generating a broad and multispecific response to infection.