gebhart/patrepeval huggingface dataset:
Inhibitory effect of Qinling granule on duck hepatitis B virus hepatitis model in nestling ducks in vivo (1999)

AIM To study the inhibitory effect of Qinling granule(QG) on duck hepatitis B virus (DHBV) hepatitis model innestling ducks in vivo.METHODS Seven-day--old DHBV infected nestling duckswere randomly divided into five groups: normal saline(NS); positive control drug, ACV 100 mg/ kg; QG10 g/ kg; QG 5g/ kg; and QG 2.5 g/ kg. Five animalswere used in each group. Drugs were administered orally,twice daily for 10 days. Blood was taken from leg vein andwere separated before treatment (TO), on day 5 and day10 (T5,T10) after treatment, and on day 3 (P3) after termination of treatment. The presence of DHBV DNA in serum was detected by dot-blot hybridization. Probe was labelled with 32 P. Livers were cut out for pathological examination.RESUI.TS In middle and high dosage groups of QG (5 and10 g/ kg), serum DHBV--DNA levels were significantly lowered (P0. 05 and P 0 .01 ), and the effect was similar inlow dose group.CONCLUSION Qingling granule can inhibit DHBV in vivo.It is worthy of further studies as an anti--hepatitis drug.
Human breast carcinoma patients develop clonable oncofetal antigen-specific effector and regulatory T lymphocytes. (1999)

Oncofetal Ag (OFA) is a 44-kDa glycoprotein expressed during early to mid-gestation fetal development and re-expressed as a surface Ag by tumor cells soon after transformation. The Ag is detectable on all types of human and rodent tumors tested, but is undetectable on normal cells. In experimental animals it is autoimmunogenic and induces potentially protective T cell responses both after experimental immunization and during tumor development subsequent to carcinogenic insult. To determine whether this tumor-associated Ag is also immunogenic for human T lymphocytes, breast carcinoma patients' peripheral blood mononuclear leucocytes were stimulated in vitro with autologous tumor cells in the presence of IL-2, gamma-IFN, and IL-6 for 2 wk. The tumor-reactive cells were then restimulated and cloned by limiting dilution, and the clones were analyzed. We established 24, 19, 11, and 16 tumor-reactive clones from the four respective patients. Of those, 4, 6, 4, and 7, respectively, proliferated specifically to purified OFA. Both CD4 and CD8 OFA-specific clones were established, which responded equally well to purified OFA or 32- to 44-kDa immature laminin receptor protein. All were CD3+, TCR-alpha beta+. All CD4 clones secreted gamma-IFN, but neither secreted IL-4 nor IL-10. Both IFN-gamma-secreting cytotoxic CD8 clones and IL-10-secreting inhibitory CD8 clones were established. Thus, during human cancer development, the same types of OFA-specific effector and regulatory T cells are induced as during murine T lymphomagenesis.
[Effect of wuzi yanzong liquid on hypothalamus, monoamines, sexual hormones and reproductivity in male rats]. (1993)

About 100 male SD rats aged 2, 5, 10, 18 and 24 months (each age group for about 20 rats) were used in the anti-senility experiment. The animals of each age group were further subdivided into two groups randomly: one group administered with Wuzi Yanzong Liquid (WYL) daily for 5 weeks, and the other group with normal saline. After treatment, plasma testosterone (T) and estradiol (E2), hypothalamus norepinephrine (NE), dopamine (DA) and serotonin (5-HT) levels were determined. In comparison with younger groups, the 18 and 24 months old rats administered with saline had markedly lowered plasma T, hypothalamus NE and DA levels, and elevated 5-HT, E2 levels and 5-HT/DA, E2/T ratios; while in the 18 and 24 months rats administered with WYL the above mentioned changes were much less remarkable. Since WYL only raised plasma T level in old rats but not in younger ones, this drug seems to have different action from testosterone preparations. In another animal experiment, the reproduction promoting effect of WYL was shown by the increase of sperm count and motility of sperms in old male rats as well as its reproductive ability.
Amniotic Membranes as Dressings following Facial Dermabrasion (1982)

Facial dermabrasion produces a raw, painful, partial-thickness wound, quite similar to a split-thickness skin graft donor site. The various methods of dressing such wounds employing ointments, impregnated gauze, bulky absorptive dressings, xenografts, or allografts are time consuming, uncomfortable for the patient, and not infrequently characterized by localized purulence and delayed healing. Experience with amniotic membranes as biologic dressings, both experimental and clinical, prompted a trial of these membranes as a dressing following facial dermabrasion. Thirty-three patients undergoing facial dermabrasion were dressed with amniotic membranes following the procedures. The results following this dressing method were excellent. The biologic basis and the techniques of preparing and applying amniotic membranes as dressings following facial dermabrasion are presented. The advantages of amniotic membranes over the other presently employed dressing techniques following facial dermabrasion are discussed.
Oxidative damage and mitochondrial dysfunction in neurodegenerative diseases (1994)

Conference Article| November 01 1994 Oxidative damage and mitochondrial dysfunction in neurodegenerative diseases Susan E. Browne; Susan E. Browne 1Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar M. F. Beal M. F. Beal * *To whom correspondence should be addressed, at: Neurology Research, Warren 408, Massachusetts General Hospital, Fruit St., Boston, MA 02114, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1994) 22 (4): 1002–1006. https://doi.org/10.1042/bst0221002 Article history Received: June 13 1994 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation Susan E. Browne, M. F. Beal; Oxidative damage and mitochondrial dysfunction in neurodegenerative diseases. Biochem Soc Trans 1 November 1994; 22 (4): 1002–1006. doi: https://doi.org/10.1042/bst0221002 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: AD, Alzheimer's disease, ALS, amyotrophic lateral sclerosis, 3-AP, 3-acetylpyridine, DMPO, 5,5-dimethyl-1-pyrroline-N-oxide, HD, Huntington's disease, MPP+, 1-methyl-4-phenylpyridinium, mtDNA, mitochondrial DNA, NMDA, N-methyl-d-aspartate, 3-NP, 3-nitropropionic acid, OH8dG, 8-hydroxy-deoxyguanosine This content is only available as a PDF. © 1994 Biochemical Society1994 Article PDF first page preview Close Modal You do not currently have access to this content.
Phosphatidylethanolamine augments factor VIIa-tissue factor activity: enhancement of sensitivity to phosphatidylserine (1995)

The effect of phosphatidylethanolamine (PE) on the activity of the factor VIIa-tissue factor complex (fVIIa-TF) has been examined with respect to plasma clotting activity and activation of factor X (fX) in a purified system. Vesicles prepared by relipidating membrane-anchored TF (dcTF; TF1-244, lacking the C-terminal cytoplasmic tail) into phospholipid vesicles containing 6 mol % phosphatidylserine (PS) and increasing levels of PE up to 40 mol % (the balance consisting of phosphatidylcholine) were found to progressively shorten TF-initiated clotting in normal human plasma to levels comparable to those observed using dcTF relipidated with cephalin. The shortened clotting times were at least in part due to the ability of PE-containing membranes to better support the activation of fX by the fVIIa.TF complex, as vesicles with increased PE content yielded progressively higher initial rates of fX activation. Surprisingly, PE substantially altered the sensitivity of fX activation to low levels of PS, yielding near-maximal rates of activation at only 3 mol % PS compared to 15-20 mol % PS required in the absence of PE. The effect of PE was not synergistic with that of PS since PE did not increase fX activation rates at high levels of PS (20 mol %). Examination of the kinetic parameters for fX activation revealed that the majority of the effect of PE was in decreasing the apparent Km for fX.(ABSTRACT TRUNCATED AT 250 WORDS)
Repair function in organ cultured human cartilage. Replacement of enzymatically removed proteoglycans during longterm organ culture. (1985)

The effect of enzymatic removal of proteoglycans (PG) from the extracellular matrix of human articular cartilage on subsequent PG metabolism of the remaining chondrocytes was investigated for periods of up to 12 weeks. Chondrocytes resynthesized normally aggregated PG but these macromolecules failed to stabilize in the intercellular matrix of the organ cultured explants and consequently diffused into the nutrient medium. After being in culture for about 2 months the chondrocytes no longer compensated for this PG loss and ceased to synthesize these biopolymers. A shift towards hyaluronic acid synthesis--as seen in dedifferentiated chondrocyte cultures--was not observed during the culture period. In this model some attempts at repair were demonstrated.
Expression of a Soluble Complement Inhibitor Protects Transgenic Mice from Antibody-Induced Acute Renal Failure (2001)

Crry is a potent complement regulator in rodents that inhibits C3 convertases. In rats, intrarenal arterial injection of anti-glomerular endothelial cell (GEN) antibodies leads to complement-dependent microvascular injury and acute renal failure. In this study, a mouse variant of this model and the effects of complement inhibition were examined. Transgenic mice that overexpressed soluble Crry systemically and in their kidneys were studied. Anti-GEN IgG was injected intravenously into eight Crry transgenic mice and seven transgene-negative littermates (which were used as control animals). Thirty h after injection, blood urea nitrogen (BUN) levels were 30.3 +/- 4.4 and 114.8 +/- 23.5 mg/dl for transgene-positive and -negative animals, respectively (P = 0.012). Four of five transgene-negative animals with BUN levels of > 100 mg/dl were anuric; the remaining animal exhibited minimal albuminuria and no detectable urinary C3. In animals with renal failure, glomerular capillary collapse and tubular necrosis were observed. There was significant tubular staining for C3 in transgene-negative animals, with cellular and basal distributions, both of which were statistically greater than those in transgene-positive animals. Tubular cell C3 staining was strongly correlated with BUN values (r = 0.83, P < 0.001), as was C9 staining (r = 0.56, P = 0.037), suggesting that complement activation to the C5b-9 membrane attack complex had a casual role in renal failure. Thus, systemic injection of anti-GEN antibodies into mice leads to acute renal failure, with glomerular and tubular injury. Animals that overexpress soluble Crry in renal tubules and elsewhere are protected from the acute renal failure that occurs in this model, which ultimately seems to develop because of complement activation focused on tubules.
THE ROLE OF NITROGEN NUTRITION IN HIGH‐TEMPERATURE TOLERANCE OF THE KELP, LAMINARIA SACCHARINA (CHROMOPHYTA)1 (1997)

ABSTRACT Mechanisms of high‐temperature tolerance in the kelp Laminaria saccharina (L.) Lamour. were examined by comparing a heat‐tolerant ecotype from Long Island Sound (LIS), New York, and a population from the Atlantic (ATL) coast of Maine. Greater heat tolerance was not attributable to greater thermal stability of the photosynthetic apparatus: LIS and ATL plants exhibited similar short‐term effects of high temperature on photosynthetic capacity (P max ) and quantum yield (estimated as the ratio of variable to maximum chlorophyll fluorescence, F v /F m . As LIS plants had consistently higher N and protein content than ATL plants, the interaction between nitrogen nutrition and high‐temperature tolerance was examined. When grown under high N supply and optimal temperature (12° C), LIS plants had a higher density of photosystem II reaction centers (RCII), higher activity of two Calvin cycle enzymes (ribulose bisphosphate carboxylase oxygenase [RUBISCO] and NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase [G3PDH]), and higher P max and F v /F m than ATL plants. Individual ATL plants, furthermore, exhibited close correlations of RCII density and enzyme activity with N and/or protein content. Variation in RCII density and enzyme activity, in turn, largely accounted for plant‐to‐plant differences in P max and F v /F m . Relationships among these parameters were generally weak or lacking among individual LIS plants grown under optimal conditions, apparently because luxury N consumption resulted in excess reserves of photosynthetic apparatus components. Exposure of N‐replete LIS and ATL plants to a superoptimal temperature (22° C) for 4 days caused an increase in the minimum turnover time of the photosynthetic apparatus (tau) and a decrease in P max , but had no consistent effect on F v /F m RCII density, PSU size (chlorophyll a/RCII), or enzyme activities. When plants were subjected to concurrent N limitation and heat stress, however, LIS and ATL populations exhibited quite different responses. All photosynthetic parameters of N‐limited ATL plants declined sharply in response to high temperature, resulting in a negative rate of daily net C fixation. In contrast, LIS plants showed a reduction in PSU size, but maintained other parameters, including daily C fixation, at levels similar to those of N‐limited plants at optimal temperature. Overall, the ability of LIS plants to accumulate and maintain high N reserves appears to be critical for heat tolerance and, therefore, for survival during summer periods of simultaneous low N supply and superoptimal temperature. ATL plants, which also experience low summer N supply but not superoptimal temperatures, do not accumulate large reserves of nitrogenous components and are unable to tolerate the combined stress. Because low N supply often co‐occurs with high temperatures in temperate marine systems, large‐scale declines in algal productivity, such as during El Niño events, are probably due to the interactive effect of N limitation and heat stress.
VLSI architectures for multiplication in GF(2/sup m/) for application tailored digital signal processors (2002)

Finite field arithmetic plays an important role in coding theory, cryptography and their applications. Several hardware solutions using finite field arithmetic have already been developed but none of them are user programmable. This is probably one reason why BCH codes are not commonly used in mobile communication applications even though these codes have very desirable properties regarding burst error correction. This article presents architectures for multiplication in GF(2/sup m/) applicable to digital signal processors. First a method is proposed to build an array of gates for hardware multiplication in GF(2/sup m/). Then an approach is shown that combines the hardware of a typical standard binary arithmetic multiplier with a GF(2/sup m/) multiplier. Using this approach saves a considerable number of gates and decreases the bus load while increasing the latency of the standard binary multiplier unit only marginally. Finally, a solution of a combined 17/spl times/17 integer/GF(2/sup m/spl les/8/) multiplier is presented and discussed.
A note on -conformally flat contact manifolds. (2006)

We prove that a contact manifold with the structure vector field ξ belonging to the k-nullity distribution is ξ -conformally flat if and only if it is an η-Einstein manifold and we give some applications. 2000 Mathematics Subject Classification: 53C15, 53C25
Gaba in the nervous system : the view at fifty years (2000)

Adventures with GABA:Fifty Years On Mechanisms Controlling GABA Synthesis and Degradation in the Brain Cytoplasmic Membrane Transporters for GABA Uptake and Storage of GABA in Synaptic Vesicles GABA Chemistry:Analogs of GABA as Therapeutic and Investigational Agents Function of GABA Receptors:Insights from Mutant and Knockout Mice The Benzodiazepine Site of GABA Receptors Pharmacologic Subtypes of GABA Receptors Based on Subunit Composition Functional Domains of GABA Receptors Kinetic Properties of GABA Receptor Channels Alcohol and General Anesthetic Actions on the GABA Receptor Regulation of GABA Receptors by Protein Phosphorylation Neurosteroids and GABA Receptor Plasticity GABA Receptors:Structure and Function GABA Emerges as a Developmental Signal during Neurogenesis of the Rat Central Nervous System GABA in the Basal Ganglia Epilepsy GABAergic Interneuronal Networks in the Hippocampus GABA Neurons of the Hippocampal Formation:Localization, Vulnerability, and Plasticity GABA Neurons of the Neocortex GABA Receptor Diversity:A View from the Cerebellum GABAergic Inhibition in the Cerebellar System Neuromodulation of GABA in Developing and Mature Hypothalamic Neurons GABA in the Mammalian Spinal Cord GABA in the Retina The Role of Glutamic Acid Decarboxylase and GABA in the Pancreas and Diabetes Index
Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR (1995)

Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H7 strains were identified simultaneously with the SLT types encoded by these strains.
A CONVENIENT METHOD FOR THE DIRECT PREPARATION OF KETONES FROM 2-(6-(2-METHOXYETHYL)PYRIDYL)CARBOXYLATES AND ALKYL IODIDES BY USE OF ZINC DUST AND A CATALYTIC AMOUNT OF NICKEL DICHLORIDE (1981)

Abstract Treatment of 2-(6-(2-methoxyethyl)pyridyl) carboxylates with alkyl iodides in the presence of zinc dust and a catalytic amount of NiCl2 in DMF at 50 °C affords unsymmetrical ketones in good yields by a one-pot procedure.
Human Leukocyte Antigen-G Expression After Heart Transplantation Is Associated With a Reduced Incidence of Rejection (2002)

There is a significant correlation between rejection and HLA-G expression in the heart after transplantation. HLA-G expression and its effect in reducing the incidence and severity of rejection seem to be stable throughout the evolution.
Synthesis of benzo[1,2-d;3,4-d′]diimidazole and 1H-pyrazolo[4,3-b]pyridine as putative A2A receptor antagonists (2007)

The synthesis and the binding affinity for the putative adenosine receptor antagonist 6-methyl-7-[1,2,3]triazol-2-yl-1,6-dihydrobenzo[1,2-d;3,4-d']diimidazole (10) and 5-oxazol-2-yl-1H-pyrazolo[4,3-b]pyridin-3-ylamine (16) are reported. The title compounds were prepared from commercially available 1-chloro-2,4-dinitrobenzene (1) and 2-chloro-6-methoxy-3nitropyridine (11), respectively, but proved devoid of affinity for the adenosine A1 and A2A receptors.
Stability and assembly in vitro of bacteriophage PP7 virus-like particles (2007)

The presence of inter-dimer disulfide bonds greatly stabilizes the PP7 virus-like particle against thermal denaturation. Covalently cross-linking the subunits of the dimers themselves by genetically fusing them through a dipeptide linker sequence, offers no further stabilization of the VLP, although it does stabilize the dimer. PP7 capsids readily assemble in vitro in a reaction that requires RNA.
Totally syntheticanalogues of siastatin B. III. Trifluoroacetamide analogues having inhibitory activity for tumor metastasis. (1994)

A trifluoroacetamide analogue of siastatin B, (3S,4S,5R,6R)-6-(trifluoroacetamido)-4,5-dihydroxy-3-piperidine carboxylic acid has been chemically synthesized. This compound, as well as the previously synthesized analogue, (3R,4R,5R,6R)-6-(trifluoroacetamido)-3,4,5-trihydroxy-3-piperid inecarboxylic acid, showed marked inhibitory activity against beta-glucuronidase and significant inhibition of experimental pulmonary metastasis of the highly metastatic melanoma B16.
Hypomethylation Status of CpG Sites at the Promoter Region and Overexpression of the Human MDR1 Gene in Acute Myeloid Leukemias (1998)

Abstract Selection of human cells for resistance to vincristine or doxorubicin often induces overexpression of the multidrug resistance 1 gene (MDR1), which encodes the cell surface P-glycoprotein, as a result of gene amplification or transcriptional activation. Moreover, overexpression of the MDR1 gene has been shown to be associated closely with clinical outcome in various hematological malignancies, including acute myeloid leukemia (AML). However, the precise mechanism underlying overexpression of the MDR1 gene during acquisition of drug resistance remains unclear. We recently described an inverse correlation between the methylation status of CpG sites at the promoter region and expression of the MDR1 gene in malignant cell lines. In this study, we expanded this analysis to 42 clinical AML samples. We adapted a quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay for gene expression and a quantitative PCR after digestion by Hpa II for methylation status of the MDR1gene. We observed a statistically significant inverse correlation between methylation and MDR1 expression in clinical samples. The hypomethylation status of the MDR1 promoter region might be a necessary condition for MDR1 gene overexpression and establishment of P-glycoprotein–mediated multidrug resistance in AML patients.
Characterization of quantitative mucin variants from a human colon cancer cell line. (1987)

Colonic mucins are high molecular weight glycoproteins produced by goblet cells of colonic epithelium. Some studies have indicated that patients with colonic cancers that produce high amounts of mucin have a poorer prognosis than patients whose tumors produce low amounts of mucin. At present, however, the role of mucin in affecting the behavior of colon cancer cells is not well understood. To further elucidate the relationship between cellular mucin content and the growth characteristics and morphology of tumor cells, we utilized a replica plating technique and immunoscreening method to identify and purify variant clones of the human colon cancer cell line LS174T that produce high and low levels of mucin. This procedure enabled us to isolate two high mucin-containing variants (HM3 and HM7) and one low mucin-containing variant (LM12). These variants exhibited different morphology. Both high mucin variants tended to form cell aggregates and suspended cells with adjoining mucoid threads. The low mucin variant formed spread monolayers on the substratum with the formation of cell processes. Metabolic labeling using [3H]glucosamine demonstrated that high mucin variants synthesized 2-fold more mucin in the cell layer and secreted 3-fold more mucin into the culture medium than the low mucin variant. The colony-forming efficiency in semisolid agar for these variants positively correlated with their mucin content. High mucin variant cells when injected into athymic nude mice formed tumors 2-fold larger than those of the parental cells while the low mucin variant formed tumors only one-half as large as those of the parental cell line. These mucin variants should provide a useful model for understanding the biological behavior of mucinous colon cancer cells in vivo and in vitro.