gebhart/patrepeval huggingface dataset:
Mechanisms of Neointima Formation—Lessons from Experimental Models (1997)

The lamina intima of an artery is the region between the endothelial cell surface and the internal elastic lamina, which forms the luminal margin of the media. In humans the intima of atherosclerosis-prone arteries becomes thicker due to accumulation of smooth muscle cells, which originate from the media. The introduction of percutaneous transluminal coronary angioplasty (PTCA) boosted scientific interest in intimal thickening, because restenosis remains an unresolved problem of this intervention. In order to unravel the mechanisms of intimal thickening there is a need for appropriate animal models. A brief overview of these models is given together with factors that control proliferation and/or migration. Despite intensive research on neointima formation, an effective therapy for restenosis has not emerged to date. This may be due to the fact that other processes, such as acute elastic recoil and chronic constrictive remodeling may contribute to lumen narrowing as well. Other limitations of neointima models are related to species and anatomical differences. Most studies are performed in arteries that are either lesion-free, or contain relatively mild plaques, in contrast to the complicated, stenotic lesions that are the substrate for human PTCA. Other differences are the severity of the injury and incorporation of a mural fibrin-rich thrombus. Nevertheless, studies based on superficial injury, like the frequently used balloon denudation model, are useful. There are similarities with angioplasty, such as endothelial cell damage and proliferation of medial and intimal smooth muscle cells. The use of techniques such as differential display, gene transfer and application of antisense oligonucleotides may provide new therapeutic approaches to reduce neointima formation.
An Ontology Capturing the Interdependence of the General Data Protection Regulation (GDPR) and Information Security (2018)

High returns for processing personal data and low penalties for privacy violations led to the circumstance that protection of privacy was often not considered a priority. To counter this habit and to harmonize data protection laws throughout the European Union, the EU-Commission has adopted the General Data Protection Regulation (GDPR), clarifying data subject rights and ensuring an appropriate level of privacy protection.
Direct Assay for Cobalamin Bound to Transcobalamin (Holo-Transcobalamin) in Serum (2002)

The solid-phase capture assay provides a simple, reliable method for quantitative determination of holo-transcobalamin in serum.
Simian-Human Immunodeficiency Virus SHIV89.6-Induced Protection against Intravaginal Challenge with Pathogenic SIVmac239 Is Independent of the Route of Immunization and Is Associated with a Combination of Cytotoxic T-Lymphocyte and Alpha Interferon Responses (2003)

Attenuated primate lentivirus vaccines provide the most consistent protection against challenge with pathogenic simian immunodeficiency virus (SIV). Thus, they provide an excellent model to examine the influence of the route of immunization on challenge outcome and to study vaccine-induced protective anti-SIV immune responses. In the present study, rhesus macaques were immunized with live nonpathogenic simian-human immunodeficiency virus (SHIV) 89.6 either intravenously or mucosally (intranasally or intravaginally) and then challenged intravaginally with pathogenic SIVmac239. The route of immunization did not affect mucosal challenge outcome after a prolonged period of systemic infection with the nonpathogenic vaccine virus. Further, protection from the SIV challenge was associated with the induction of multiple host immune effector mechanisms. A comparison of immune responses in vaccinated-protected and vaccinated-unprotected animals revealed that vaccinated-protected animals had higher frequencies of SIV Gag-specific cytotoxic T lymphocytes and gamma interferon (IFN-gamma)-secreting cells during the acute phase postchallenge. Vaccinated-protected animals also had a more pronounced increase in peripheral blood mononuclear cell IFN-alpha mRNA levels than did the vaccinated-unprotected animals in the first few weeks after challenge. Thus, innate as well as cellular anti-SIV immune responses appeared to contribute to the SHIV89.6-induced protection against intravaginal challenge with pathogenic SIVmac239.
Axonal protection by Nmnat3 overexpression with involvement of autophagy in optic nerve degeneration (2013)

Axonal degeneration often leads to the death of neuronal cell bodies. Previous studies demonstrated the crucial role of nicotinamide mononucleotide adenylyltransferase (Nmnat) 1, 2, and 3 in axonal protection. In this study, Nmnat3 immunoreactivity was observed inside axons in the optic nerve. Overexpression of Nmnat3 exerts axonal protection against tumor necrosis factor-induced and intraocular pressure (IOP) elevation-induced optic nerve degeneration. Immunoblot analysis showed that both p62 and microtubule-associated protein light chain 3 (LC3)-II were upregulated in the optic nerve after IOP elevation. Nmnat3 transfection decreased p62 and increased LC3-II in the optic nerve both with and without experimental glaucoma. Electron microscopy showed the existence of autophagic vacuoles in optic nerve axons in the glaucoma, glaucoma+Nmnat3 transfection, and glaucoma+rapamycin groups, although preserved myelin and microtubule structures were noted in the glaucoma+Nmnat3 transfection and glaucoma+rapamycin groups. The axonal-protective effect of Nmnat3 was inhibited by 3-methyladenine, whereas rapamycin exerted axonal protection after IOP elevation. We found that p62 was present in the mitochondria and confirmed substantial colocalization of mitochondrial Nmnat3 and p62 in starved retinal ganglion cell (RGC)-5 cells. Nmnat3 transfection decreased p62 and increased autophagic flux in RGC-5 cells. These results suggest that the axonal-protective effect of Nmnat3 may be involved in autophagy machinery, and that modulation of Nmnat3 and autophagy may lead to potential strategies against degenerative optic nerve disease.
Sharp exciton emission from single InAs quantum dots in GaAs nanowires (2003)

We have performed photoluminescence spectroscopy on single GaAs nanowires with InAs quantum dots in the form of thin slices of InAs, possibly alloyed with Ga as InGaAs, incorporated into the GaAs. The nanowires were grown by chemical beam epitaxy using gold nanoparticles as catalysts. The photoluminescence measurements showed rich spectra consisting of sharp lines with energies and excitation power dependency behavior very similar to that observed for Stranski–Krastanow-grown InAs/GaAs quantum dots. By reducing the excitation power density we were able to obtain a quantum dot spectrum consisting of only one single sharp line—the exciton line.
Ist die Konformation eines Moleküls im Kristall und in Lösung gleich? NMR-Befunde an Festkörper und Lösung im Vergleich zur Röntgen-Strukturbestimmung (1981)

Der Vergleich von Strukturen im Festkörper und in Lösung mit einer Methode – der NMR-Spektroskopie – ermöglichte den zweifelsfreien Nachweis von Strukturänderungen beim Lösen einer festen Verbindung. So ist z. B. im Kristall das untersuchte vinyloge Amid (R = C6H11) an der CC-Doppelbindung E-konfiguriert; in Lösung ist eine Z-Form am stabilsten.
Versatile endless optical polarization controller/tracker/demultiplexer (2014)

Following an initial discussion of control error signal generation, we present new developments and applications of automatic endless optical polarization control based on a commercial electrooptic LiNbO(3) polarization transformer: (i) Fast tracking and subsequent demultiplexing of DPSK/DQPSK/QAM polarization channels was hitherto limited to a fairly fixed optical input power. With APD photoreceivers used for residual interference detection, we demonstrate here an optical level tolerance of at least 7 dB, compared to only 3 dB for PIN photoreceivers. DPSK channel polarizations are tracked at up to 40 krad/s and higher speed on the Poincaré sphere. (ii) High-order optical modulation schemes require increased accuracy of the polarization controller in the demultiplexer. This is possible at the expense of a reduced tracking speed. We achieve a mean polarization extinction ratio of >40 dB or <0.02 rad error while tracking arbitrary endless polarization changes of up to 1000 rad/s. (iii) While electronic polarization tracking in coherent receivers is currently limited to a symbol rate of about 28 GBaud we show optical polarization tracking of a signal with 1 THz bandwidth.
Level 5 Multilateral Completed on Alaska's North Slope: A Case History (1999)

Abstract Recent advances in multilateral technology have broadened the perspective of both re-entry and grass root wells. New tool designs, procedures, and completion equipment allow increased possibilities and functionality of multilateral wells. Multiple reservoir penetrations of a single or several reservoirs are possible from one well. Injection or production can be controlled in each leg of the well by means of downhole chokes or independent production strings. Advantages of this technology include, reduced surface footprint in environmentally sensitive or space confined areas, lower capital expenditures on surface facilities and existing wellbore infrastructure, and elimination of drilling new wells through potentially hazardous formations. In late 1995 ARCO Alaska, Inc. began to evaluate multilateral technology and its application for adding value in the Kuparuk River Field on the North Slope of Alaska. The incentive for bringing multilateral technology to Kuparuk was access to smaller pockets of reserves, not economically recoverable at new well costs. This paper provides an in depth review of the first level 5 multilateral well constructed on the North Slope. Discussed are characteristics of a level 5 multilateral and the reasons this wellbore configuration was needed in Kuparuk. The discussion portrays equipment and procedures developed to accomplish well construction and provides a detailed review of the actual operations, with lessons learned during the process.
Chronic lymphocytic leukemia incidence is substantially higher than estimated from tumor registry data (2001)

The incidence of CLL in the CAVHS was 37.6% higher than estimated from tumor registry data due to an increase in the use of peripheral blood immunophenotype as the only diagnostic test for CLL over the time period of the study. These data suggest that the true incidence of CLL may be substantially higher than estimated from tumor registry data.
Manufacture of Clinical-Grade CD19-Specific T Cells Stably Expressing Chimeric Antigen Receptor Using Sleeping Beauty System and Artificial Antigen Presenting Cells (2013)

Adoptive transfer of T cells expressing a CD19-specific chimeric antigen receptor (CAR) is being evaluated in multiple clinical trials. Our current approach to adoptive immunotherapy is based on a second generation CAR (designated CD19RCD28) that signals through a CD28 and CD3-ζ endodomain. T cells are electroporated with DNA plasmids from the Sleeping Beauty (SB) transposon/transposase system to express this CAR. Stable integrants of genetically modified T cells can then be retrieved when co-cultured with designer artificial antigen presenting cells (aAPC) in the presence of interleukin (IL)-2 and 21. Here, we reveal how the platform technologies of SB-mediated transposition and CAR-dependent propagation on aAPC were adapted for human application. Indeed, we have initiated clinical trials in patients with high-risk B-lineage malignancies undergoing autologous and allogeneic hematopoietic stem-cell transplantation (HSCT). We describe the process to manufacture clinical grade CD19-specific T cells derived from healthy donors. Three validation runs were completed in compliance with current good manufacturing practice for Phase I/II trials demonstrating that by 28 days of co-culture on γ-irradiated aAPC ∼10(10) T cells were produced of which >95% expressed CAR. These genetically modified and propagated T cells met all quality control testing and release criteria in support of infusion.
Multistation multiparameter flow cytometry: A critical review and rationale (1983)

The capacity for fluorescence excitation by beams of different wavelengths at separate points along the sample stream, and the capacity for computer analysis of multiparameter data thus obtained, are now available in flow cytometer/sorter systems from commercial producers. It is now readily apparent to most experienced users of flow cytometers that such multiparameter analysis offers the most convenient solution to the problem of characterizing subpopulations of cells within a mixed population. The use of multiple beams facilitates resolution of fluorescence signals from several probes within or upon a single cell and widens the range of analytical alternatives available to experimenters. This critical review discusses the history of the instrumentation, the parameters now measurable and the probes used for their measurement, and the methods for data analysis. Required sensitivity and precision are discussed, leading to the conclusion that many of the advantages of multistation, multiparameter flow cytometry can be made available in less complex and less costly instruments using less powerful sources and less elaborate computer hardware than are presently incorporated in commercial apparatus.
Intrinsic Defects and the Failure Properties of cis-1,4-Polyisoprenes (1996)

Abstract Inherent flaw sizes were determined from fatigue lifetimes, and from the crack length dependence of the strain energy to break, for four cis -1,4-polyisoprenes compounded to have the same crosslink density and low strain hysteresis. Both techniques indicated that the flaws intrinsic to guayule rubber (GR), and to a lesser extent conventional natural rubber, are larger than those found in deproteinized NR. This result may not be surprising; however, the failure properties of the elastomers, expected to depend on flaw size, were surprising. The guayule rubber and a natural rubber of relatively low purity (SMR-10) had the highest tensile strengths, tear strengths, breaking energies, and fatigue lifetimes, while DPNR exhibited the worst failure properties. Such an inverse correlation between flaw size and failure performance is due to the dominant effect of strain-induced crystallization. GR and SMR-10 have the highest propensity for strain-induced crystallization, while DPNR is the least strain-crystallizable. Interestingly, all rubbers exhibited the same isotropic crystallization behavior.
Targeted genome regulation via synthetic programmable transcriptional regulators (2016)

Regulation of gene transcription controls cellular functions and coordinates responses to developmental, physiological and environmental cues. Precise and efficient molecular tools are needed to characterize the functions of single and multiple genes in linear and interacting pathways in a native context. Modular DNA-binding domains from zinc fingers (ZFs) and transcriptional activator-like proteins (TALE) are amenable to bioengineering to bind DNA target sequences of interest. As a result, ZF and TALE proteins were used to develop synthetic programmable transcription factors. However, these systems are limited by the requirement to re-engineer proteins for each new target sequence. The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR associated 9 (Cas9) genome editing tool was recently repurposed for targeted transcriptional regulation by inactivation of the nuclease activity of Cas9. Due to the facile engineering, simplicity, precision and amenability to library construction, the CRISPR/Cas9 system is poised to revolutionize the functional genomics field across diverse eukaryotic species. In this review, we discuss the development of synthetic customizable transcriptional regulators and provide insights into their current and potential applications, with special emphasis on plant systems, in characterization of gene functions, elucidation of molecular mechanisms and their biotechnological applications.
Phase III evaluation of four doses of megestrol acetate as therapy for patients with cancer anorexia and/or cachexia. (1993)

The positive dose-response effect that we observed for megestrol acetate on appetite stimulation supports both our prestudy hypothesis and other available literature. Nonetheless, based primarily on the cost and inconvenience associated with the use of higher doses of this drug, it is reasonable to use 160 mg/d for the initial treatment of cancer anorexia/cachexia in routine clinical practice.
Cystic Fibrosis Liver Disease (2012)

On the whole, CFLD can be classified into 3 stages:
The essence of command injection attacks in web applications (2006)

Web applications typically interact with a back-end database to retrieve persistent data and then present the data to the user as dynamically generated output, such as HTML web pages. However, this interaction is commonly done through a low-level API by dynamically constructing query strings within a general-purpose programming language, such as Java. This low-level interaction is ad hoc because it does not take into account the structure of the output language. Accordingly, user inputs are treated as isolated lexical entities which, if not properly sanitized, can cause the web application to generate unintended output. This is called a command injection attack, which poses a serious threat to web application security. This paper presents the first formal definition of command injection attacks in the context of web applications, and gives a sound and complete algorithm for preventing them based on context-free grammars and compiler parsing techniques. Our key observation is that, for an attack to succeed, the input that gets propagated into the database query or the output document must change the intended syntactic structure of the query or document. Our definition and algorithm are general and apply to many forms of command injection attacks. We validate our approach with SqlCheckS, an implementation for the setting of SQL command injection attacks. We evaluated SqlCheckS on real-world web applications with systematically compiled real-world attack data as input. SqlCheckS produced no false positives or false negatives, incurred low runtime overhead, and applied straightforwardly to web applications written in different languages.
Serendipity in analytical chemistry (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSerendipity in analytical chemistryPeter F. LottCite this: Anal. Chem. 1983, 55, 2, 245–248Publication Date (Print):February 1, 1983Publication History Published online1 May 2002Published inissue 1 February 1983https://pubs.acs.org/doi/10.1021/ac00253a002https://doi.org/10.1021/ac00253a002research-articleACS PublicationsRequest reuse permissionsArticle Views89Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Reaction of serine proteases with substituted 3-alkoxy-4-chloroisocoumarins and 3-alkoxy-7-amino-4-chloroisocoumarins: new reactive mechanism-based inhibitors (1985)

The time-dependent inactivation of several serine proteases including human leukocyte elastase, cathepsin G, rat mast cell proteases I and II, and human skin chymase by a number of 3-alkoxy-4-chloroisocoumarins, 3-alkoxy-4-chloro-7-nitroisocoumarins, and 3-alkoxy-7-amino-4-chloroisocoumarins at pH 7.5 and the inactivation of several trypsin-like enzymes including human thrombin and factor XIIa by 7-amino-4-chloro-3-ethoxyisocoumarin and 4-chloro-3-ethoxyisocoumarin are reported. The 3-alkoxy substituent of the isocoumarin is likely interacting with the S1 subsite of the enzyme since the most reactive inhibitor for a particular enzyme had a 3-substituent complementary to the enzyme's primary substrate specificity site (S1). Inactivation of several enzymes including human leukocyte elastase by the 3-alkoxy-7-amino-4-chlorisocoumarins is irreversible, and less than 3% activity is regained upon extensive dialysis of the inactivated enzyme. Addition of hydroxylamine to enzymes inactivated by the 3-alkoxy-7-amino-4-chloroisocoumarins results in a slow (t1/2 greater than 6.7 h) and incomplete (32-57%) regain in enzymatic activity at pH 7.5. Inactivation by the 3-alkoxy-4-chloroisocoumarins and 3-alkoxy-4-chloro-7-nitroisocoumarins on the other hand is transient, and full enzyme activity is regained rapidly either upon standing, after dialysis, or upon the addition of buffered hydroxylamine. The rate of inactivation by the substituted isocoumarins is decreased when substrates or reversible inhibitors are present in the incubation mixture, which indicates active site involvement. The inactivation rates are dependent upon the pH of the reaction mixture, the isocoumarin ring system is opened concurrently with inactivation, and the reaction of 3-alkoxy-7-amino-4-chloroisocoumarins with porcine pancreatic elastase is shown to be stoichiometric. The results are consistent with a scheme where 3-alkoxy-7-amino-4-chloroisocoumarins react with the active site serine of a serine protease to give an acyl enzyme in which a reactive quinone imine methide can be released. Irreversible inactivation could then occur upon alkylation of an active site nucleophile (probably histidine-57) by the acyl quinone imine methide. The finding that hydroxylamine slowly catalyzes partial reactivation indicates that several inactivated enzyme species may exist. The 3-alkoxy-substituted 4-chloroisocoumarins and 4-chloro-7-nitroisocoumarins are simple acylating agents and do not give stable inactivated enzyme structures.(ABSTRACT TRUNCATED AT 400 WORDS)
Space-Time Representation of People Based on 3D Skeletal Data: A Review (2016)

Spatiotemporal human representation based on 3D visual perception data is a rapidly growing research area. Based on the information sources, these representations can be broadly categorized into two groups based on RGB-D information or 3D skeleton data. Recently, skeleton-based human representations have been intensively studied and kept attracting an increasing attention, due to their robustness to variations of viewpoint, human body scale and motion speed as well as the realtime, online performance. This paper presents a comprehensive survey of existing space-time representations of people based on 3D skeletal data, and provides an informative categorization and analysis of these methods from the perspectives, including information modality, representation encoding, structure and transition, and feature engineering. We also provide a brief overview of skeleton acquisition devices and construction methods, enlist a number of public benchmark datasets with skeleton data, and discuss potential future research directions.