gebhart/patrepeval huggingface dataset:
A design methodology for operational control elements for automatic guided vehicle based material handling systems (1982)

A methodology for the design of operational control aspects of an Automatic Guided Vehicle (AGV) based material handling system is presented. The methodology, which is composed of an integrated model of an AGV based system, was implemented using simulation techniques. The model views a manufacturing function as consisting of machining, queueing, and moving of parts in a shop and that these components of manufacturing must be integrated and coordinated if the production objectives of an enterprise are to be realized. A machining center is modeled as a physical region of a plant and it consists of machines for part processing and capacitated queues in which inbound and outbound parts reside, queueing for machining or handling resources. Automatic guided vehicles provide the transport mechanism required to interface the machining centers. A network approach is employed to represent the layout of the facility, including the location of departments, input and output queues in each department, and the layout of the guidance system on which the AGVs operate. The network approach, along with the coordinate system are employed for modeling the actual translation of vehicles and parts through the shop. The travel time of vehicles and parts between points depends on vehicle speed and the prevailing traffic condition along the path of travel. Several shop control strategies in the application of AGVs have been modeled, implemented, and their effects on shop performance demonstrated. Among these factors are vehicle dispatching, vehicle routing, unit load size selection, job sequencing, shop loading, queue constraints, and capacity constraints due to vehicles and machines. A job in the shop is considered to consist of one or more parts grouped in portable unit load sizes. Therefore, it is unit loads rather than jobs that make the flow transitions. The results of the simulation experiments conducted indicated vital control elements in the design of AGV systems. Through a series of output statistics on system performance, the model provides an easy to use tool to analyze, evaluate, and design of AGV based manufacturing system.
Epsilon-logic is more expressive than first-order logic over finite structures (2000)

Abstract There are properties of finite structures that are expressible with the use of Hilbert's ∈-operator in a manner that does not depend on the actual interpretation for ∈-terms. but not expressible in plain first-order. This observation strengthens a corresponding result of Gurevich, concerning the invariant use of an auxiliary ordering in first-order logic over finite structures. The present result also implies that certain non-deterministic choice constructs, which have been considered in database theory, properly enhance the expressive power of first-order logic even as far as deterministic queries are concerned, thereby answering a question raised by Abiteboul and Vianu.
Polymer Brushes that Resist Adsorption of Model Proteins:  Design Parameters (1999)

The adsorption of model proteins onto brush-coated surfaces can occur via two modes. Primary adsorption at the surface, where short range attraction is dominant, is important for small proteins and may be repressed by increasing the grafting density. Secondary adsorption, due to van der Waals attraction, occurs at the outer edge of the brush. Large rodlike proteins are likely to adsorb in this fashion. This mode may be repressed by increasing the brush thickness. The thermodynamics and kinetics of adsorption of model proteins are considered within a simple analytical theory distinguishing between the different adsorption mechanisms of small and big proteins.
Using Advanced Encryption Standard (AES) CCM Mode with IPsec Encapsulating Security Payload (ESP) (2005)

protocol for the Internet community, and requests discussion and suggestions for improvements.
Thrombospondin‐1 mediates platelet adhesion at high shear via glycoprotein Ib (GPIb): an alternative/backup mechanism to von Willebrand factor (2003)

Acute thrombotic arterial occlusion is the leading cause of morbidity and mortality in the Western world. Von Willebrand factor is thought to be the only indispensable adhesive substrate to promote thrombus formation in high shear environments. We found that thrombospondin-1, a glycoprotein enriched in arteriosclerotic plaques, might function as an alternative substrate for thrombus formation. Platelets adhered to thrombospondin-1 in a shear dependent manner with an optimum shear as found in stenosed arteries. Adhesion is extremely firm, with no detachment of platelets up to a shear rate of 4000 s(-1). Experiments using platelets from a patient completely lacking von Willebrand factor showed that von Willebrand factor is not involved in platelet binding to thrombospondin-1. Platelet adhesion to thrombospondin-1 is not mediated via beta3-integrins or GPIa. CD36 partially mediates the adhesion of pre-activated platelets. We identified GPIb as high shear adhesion-receptor for thrombospondin-1. Soluble GPIb, as well as antibodies against the GPIb, blocked platelet adhesion almost completely. The new discovered thrombospondin-1-GPIb adhesion axis under arterial shear conditions might be important, not only during thrombus formation but also for pathological processes where other cells bind to the endothelium or subendothelium, including arteriosclerosis, inflammation and tumor metastasis, and a promising therapeutic target.
Nuclear export of OLIG2 in neural stem cells is essential for ciliary neurotrophic factor–induced astrocyte differentiation (2004)

Neural stem cell (NSC) differentiation is precisely controlled by a network of transcription factors, which themselves are regulated by extracellular signals (Bertrand, N., D.S. Castro, and F. Guillemot, 2002. Nat. Rev. Neurosci 3:517-530; Shirasaki, R. and S.L. Pfaff, 2002. Annu. Rev. Neurosci 25:251-281). One way that the activity of such transcription factors is controlled is by the regulation of their movement between the cytosol and nucleus (Vandromme, M., C. Gauthier-Rouviere, N. Lamb, and A. Fernandez, 1996. Trends Biochem.Sci. 21:59-64; Lei, E.P. and P.A. Silver, 2002. Dev. Cell 2:261-272). Here we show that the basic helix-loop-helix transcription factor OLIG2, which has been shown to be required for motor neuron and oligodendrocyte development, is found in the cytoplasm, but not the nucleus, of astrocytes in culture and of a subset of astrocytes in the subventricular zone. We demonstrate that the accumulation of OLIG2 in the nucleus of NSCs blocks the CNTF-induced astrocyte differentiation and that the translocation of OLIG2 to the cytoplasm is promoted by activated AKT. We propose that the AKT-stimulated export of OLIG2 from the nucleus of NSCs is essential for the astrocyte differentiation.
RNA interference: Antiviral weapon and beyond (2003)

RNA interference (RNAi) is a remarkable type of gene regulation based on sequence-specific targeting and degradation of RNA. The term encompasses related pathways found in a broad range of eukaryotic organisms, including fungi, plants, and animals. RNA interference is part of a sophisticated network of interconnected pathways for cellular defense, RNA surveillance, and development and it may become a powerful tool to manipulate gene expression experimentally. RNAi technology is currently being evaluated not only as an extremely powerful instrument for functional genomic analyses, but also as a potentially useful method to develop specific dsRNA based gene-silencing therapeutics. Several laboratories have been interested in using RNAi to control viral infection and many reports in Nature and in Cell show that short interfering (si) RNAs can inhibit infection by HIV-1, polio and hepatitis C viruses in a sequence-specific manner. RNA-based strategies for gene inhibition in mammalian cells have recently been described, which offer the promise of antiviral therapy.
Plaque inhibition of two commercially available chlorhexidine mouthrinses (2005)

Within the limitations of the present study design, it can be concluded that rinsing with a 0.12% CHX mouthrinse on a non-alcohol base with 0.05% Cpc (Perio-Aid) is not significantly different from rinsing with a 0.2% CHX mouthrinse on an alcohol base (Corsodyl). It appears that the subjects appreciated the taste of the non-alcohol CHX solution better but the after-taste of the rinse remained longer in the mouth.
Uso del vídeo como recurso educativo no formal en la docencia universitaria en ingeniería informática = Use of video as a non-formal educational resource in higher education teaching in computer engineering (2018)

ResumenEl extenso programa curricular de las asignaturas, en los actuales programas de grado, y las actividades asociadas suponen una limitación temporal para el docente a la hora de impartir de manera precisa los contenidos de asignaturas técnicas en grados de ingeniería, que requieren además la resolución de problemas y la dedicación de horas de laboratorio enfocadas a la realización de prácticas.Para el caso concreto de asignaturas de primer año supone un reto adicional la adaptación del estudiante que proviene de enseñanzas medias, y necesita de una mayor guía por parte del docente. Esta experiencia expone una práctica docente cuyo objetivo principal ha sido actuar en tiempo real, sobre las necesidades de los estudiantes, que cuentan con conocimientos heterogéneos, a la vez que mejorar los resultados académicos de la asignatura, que exige unas competencias básicas de pensamiento computacional de las que carecen los estudiantes.Para alcanzar este objetivo se ha optado por el uso del vídeo como refuerzo a la práctica docente, a través de un lenguaje y medio no formal como es la plataforma YouTube. Los resultados de la asignatura y en concreto el uso de los vídeos han mostrado un buen grado de una aceptación para los alumnos que los utilizaron a lo largo de toda la asignatura y una percepción de aprendizaje notable, además de obtener unos resultados satisfactorios en el número de alumnos que superaron la asignatura.AbstractThe broad curricular program of the subjects, in the current degree programs, and the associated activities suppose a temporary limitation for the teacher at the time of teaching more accurately the contents of technical subjects in degrees of engineering, which also require the resolution of problems and the dedication of laboratory hours focused on the realization of practices.For the specific case of first year subjects, it is an additional challenge to adapt the student that comes from middle school, and needs more guidance from the teacher. This experience exposes a teaching practice whose main objective has been to act in real time, on the needs of the students, who have heterogeneous knowledge, at the same time as improving the academic results of the subject, which demands some basic computational thinking skills of those that students lack.To achieve this goal, the use of video has been chosen as a reinforcement to the teaching practice, through a non-formal language and means, such as the YouTube platform. The results of the subject and in particular the use of the videos have shown an acceptance for the students who used them throughout the course and a remarkable learning perception, in addition to obtaining satisfactory results in the number of students who passed the subject.
Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement. (1989)

As human E (HuE) treated with neuraminidase (Neu) are resistant to hemolysis by human serum but are readily lysed by heterologous serum via the alternative C pathway, we attempted to produce mAb which might modify Neu-treated HuE (Neu-HuE) so as to render them sensitive to homologous C. A hybridoma, clone -1F5, was obtained by screening for antibody which caused hemolysis of Neu-HuE by human serum via the alternative C pathway. We have shown that this antibody (1F5) of IgG1 isotype blocks the action of a 20-kDa membrane inhibitor capable of interfering with the terminal step in the homologous C cascade. The antigenic molecule can be termed HRF20, which stands for homologous restriction factor (HRF) with m.w. 20,000, because its function is essentially the same as that of HRF (68,000) reported by others.
The role of tumor necrosis factor receptor superfamily members in mammalian brain development, function and homeostasis (2011)

Tumor necrosis factor receptor superfamily (TNFRSF) members were initially identified as immunological mediators, and are still commonly perceived as immunological molecules. However, our understanding of the diversity of TNFRSF members' roles in mammalian physiology has grown significantly since the first discovery of TNFRp55 (TNFRSF1) in 1975. In particular, the last decade has provided evidence for important roles in brain development, function and the emergent field of neuronal homeostasis. Recent evidence suggests that TNFRSF members are expressed in an overlapping regulated pattern during neuronal development, participating in the regulation of neuronal expansion, growth, differentiation and regional pattern development. This review examines evidence for non-immunological roles of TNFRSF members in brain development, function and maintenance under normal physiological conditions. In addition, several aspects of brain function during inflammation will also be described, when illuminating and relevant to the non-immunological role of TNFRSF members. Finally, key questions in the field will be outlined.
Specific Inhibition of the Classical Complement Pathway by C1q-Binding Peptides (2001)

Undesired activation of the complement system is a major pathogenic factor contributing to various immune complex diseases and conditions such as hyperacute xenograft rejection. We aim for prevention of complement-mediated damage by specific inhibition of the classical complement pathway, thus not affecting the antimicrobial functions of the complement system via the alternative pathway and the lectin pathway. Therefore, 42 peptides previously selected from phage-displayed peptide libraries on basis of C1q binding were synthesized and examined for their ability to inhibit the function of C1q. From seven peptides that showed inhibition of C1q hemolytic activity but no inhibition of the alternative complement pathway, one peptide (2J) was selected and further studied. Peptide 2J inhibited the hemolytic activity of C1q from human, chimpanzee, rhesus monkey, rat, and mouse origin, all with a similar dose-response relationship (IC(50) 2-6 microM). Binding of C1q to peptide 2J involved the globular head domain of C1q. In line with this interaction, peptide 2J dose-dependently inhibited the binding of C1q to IgG and blocked activation of C4 and C3 and formation of C5b-9 induced via classical pathway activation, as assessed by ELISA. Furthermore, the peptide strongly inhibited the deposition of C4 and C3 on pig cells following their exposure to human xenoreactive Abs and complement. We conclude that peptide 2J is a promising reagent for the development of a therapeutic inhibitor of the earliest step of the classical complement pathway, i.e., the binding of C1q to its target.
Structural and Functional Differences between 3-Repeat and 4-Repeat Tau Isoforms (2000)

Tau, MAP2, and MAP4 are members of a microtubule-associated protein (MAP) family that are each expressed as "3-repeat" and "4-repeat" isoforms. These isoforms arise from tightly controlled tissue-specific and/or developmentally regulated alternative splicing of a 31-amino acid long "inter-repeat:repeat module," raising the possibility that different MAP isoforms may possess some distinct functional capabilities. Consistent with this hypothesis, regulatory mutations in the human tau gene that disrupt the normal balance between 3-repeat and 4-repeat tau isoform expression lead to a collection of neurodegenerative diseases known as FTDP-17 (fronto-temporal dementias and Parkinsonism linked to chromosome 17), which are characterized by the formation of pathological tau filaments and neuronal cell death. Unfortunately, very little is known regarding structural and functional differences between the isoforms. In our previous analyses, we focused on 4-repeat tau structure and function. Here, we investigate 3-repeat tau, generating a series of truncations, amino acid substitutions, and internal deletions and examining the functional consequences. 3-Repeat tau possesses a "core microtubule binding domain" composed of its first two repeats and the intervening inter-repeat. This observation is in marked contrast to the widely held notion that tau possesses multiple independent tubulin-binding sites aligned in sequence along the length of the protein. In addition, we observed that the carboxyl-terminal sequences downstream of the repeat region make a strong but indirect contribution to microtubule binding activity in 3-repeat tau, which is in contrast to the negligible effect of these same sequences in 4-repeat tau. Taken together with previous work, these data suggest that 3-repeat and 4-repeat tau assume complex and distinct structures that are regulated differentially, which in turn suggests that they may possess isoform-specific functional capabilities. The relevance of isoform-specific structure and function to normal tau action and the onset of neurodegenerative disease are discussed.
618. Steric effects in 2: 2′-bridged diphenyls with a heterocyclic bridging ring. Part I. Optically active dihydrodibenzazepines (1958)

S. R. Ahmed and D. M. Hall, J. Chem. Soc., 1958, 3043 DOI: 10.1039/JR9580003043
Whole exome sequencing identifies a recurrentRQCD1P131L mutation in cutaneous melanoma (2014)

Melanoma is often caused by mutations due to exposure to ultraviolet radiation. This study reports a recurrent somatic C > T change causing a P131L mutation in the RQCD1 (Required for Cell Differentiation1 Homolog) gene identified through whole exome sequencing of 20 metastatic melanomas. Screening in 715 additional primary melanomas revealed a prevalence of ~4%. This represents the first reported recurrent mutation in a member of the CCR4-NOT complex in cancer. Compared to tumors without the mutation, the P131L mutant positive tumors were associated with increased thickness (p = 0.02), head and neck (p = 0.009) and upper limb (p = 0.03) location, lentigo maligna melanoma subtype (p = 0.02) and BRAF V600K (p = 0.04) but not V600E or NRAS codon 61 mutations. There was no association with nodal disease (p = 0.3). Mutually exclusive mutations of other members of the CCR4-NOT complex were found in ~20% of the TCGA melanoma dataset suggesting the complex may play an important role in melanoma biology. Mutant RQCD1 was predicted to bind strongly to HLA-A0201 and HLA-Cw3 MHC1 complexes. From thirteen patients with mutant RQCD1, an anti-tumor CD8⁺ T cell response was observed from a single patient's peripheral blood mononuclear cell population stimulated with mutated peptide compared to wildtype indicating a neoantigen may be formed.
A New Technique for the Isolation and Surface Immobilization of Mesenchymal Stem Cells from Whole Bone Marrow Using High‐Specific DNA Aptamers (2006)

Adult mesenchymal stem cells (aMSCs) are a stem cell population present in bone marrow, which can be isolated and expanded in culture and characterized. Due to the lack of specific surface markers, it is difficult to separate the MSCs from bone marrow directly. Here, we present a novel method using high-specific nucleic acids called aptamers. Porcine MSCs were used as a target to generate aptamers by combinatorial chemistry out of a huge random library with in vitro technology called systematic evolution of ligands by exponential enrichment (SELEX). After cloning and sequencing, the binding affinity was detected using a cell-sorting assay with streptavidin-coated magnetic microbeads. We also used 12-well plates immobilized with aptamers to fish out MSCs from the cell solution and a fluorescein isothiocyanate-labeled aptamer to sort MSCs from bone marrow using high-speed fluorescence-activated cell sorting. The cells showed high potency to differentiate into osteogenic, as well as into adipogenic, lineages with typical morphological characteristics. Surface marker staining showed that the attached cells were CD29(+), CD44(+), CD45(-), CD90(+), SLA class I(+), SLA DQ(-), and SLA DR(-). Compared with existing methods, this study established a novel, rapid, and efficient method for direct isolation of aMSCs from porcine bone marrow by using an aptamer as a probe to fish out the aMSCs. This new application of aptamers can facilitate aMSC isolation and enrichment greatly, thereby enhancing the rate of aMSC-derived cells after in vitro differentiation for various applications in the emerging field of tissue engineering and regenerative medicine.
Healing following implantation of periodontitis affected roots into bone tissue (1980)

The aim of the present experiment was to study whether new connective tissue attachment can occur to root surfaces which have been exposed to the oral environment and subsequently implanted into bone tissue. Twelve teeth in three beagle dogs were subjected to progressive periodontal breakdown to half the root length by placing cotton floss ligatures around the neck of the teeth. Following crown resection and root hemisection, the teeth were root filled and the roots thoroughly scaled and planed. Each root was extracted and implanted into bone cavities prepared in edentolous areas of the jaws in such a way that epithelial migration into the wound and bacterial infection were prevented during healing. Root implantation and sacrifice of the animals were scheduled to allow for observation periods of 1, 2 and 3 months. The results demonstrated that new connective tissue attachment did not occur to root surfaces which had been exposed to the oral environment, but healing was characterized by repair phenomena, i.e. mainly root resorption and ankylosis. In those areas of the roots where periodontal ligament tissue was preserved following tooth extraction, a functionally oriented attachment apparatus was reformed. The results indicate that in addition to apical migration of junctional epithelium and regrowth of subgingival plaque, the type of cells which repopulate the wound area may jeopardize new connective tissue attachment.
Heterogeneous nuclear RNA promotes synthesis of (2',5')oligoadenylate and is cleaved by the (2',5')oligoadenylate-activated endoribonuclease. (1982)

Heterogeneous nuclear RNA contains double-stranded regions that are not found in mRNA and that may serve as recognition elements for processing enzymes. The double-stranded regions of heterogeneous nuclear RNA prepared from HeLa cells promoted the synthesis of (2',5')oligoadenylate [(2',5')oligo(A) or (2'5')An] when incubated with (2',5')An polymerase. This enzyme is present in elevated levels in interferon-treated cells, and labeled heterogeneous nuclear RNA incubated with extracts of these cells is preferentially cleaved, since mRNA included in the same incubations is not appreciably degraded. The cleavage of heterogenous nuclear RNA is caused by the synthesis of (2'5')An and by a "localized" activation of the (2',5')An-dependent endonuclease, since it was enhanced by ATP, the substrate of the (2',5')An polymerase, and inhibited by 2'-dATP and ethidium bromide. Both of these compounds suppress the synthesis of (2',5')An, the first by competitive inhibition and the latter by intercalating into double-stranded RNA. The possible role of double-stranded regions and of the (2',5')An polymerase-endonuclease system in the processing of heterogeneous nuclear RNA is discussed.