gebhart/patrepeval huggingface dataset:
LA GESTIÓN FORESTAL SOSTENIBLE EN LA PROVINCIA DE ESMERALDAS, ECUADOR: UN ANÁLISIS CON ENFOQUE DE CIENCIA, TECNOLOGÍA Y SOCIEDAD (2014)

En este articulo se aborda la gestion forestal sostenible en la Provincia de Esmeraldas Ecuador, basada en los analisis de la Ciencia Tecnologia y Sociedad. La provincia de Esmeraldas es una de las mas biodiversas del Ecuadory la unica en la costa ecuatoriana donde aun se puede observar bosques primarios.En los ultimos anosdichos bosques estan siendo destruidos por la explotacion maderera (aproximadamente el 30% de la madera que se extraees comercializada ilegalmente), la agricultura itinerante yla expansion de asentamientos humanos. Esta situacion promueve acciones para mejorar la calidad de vida en el sector, creandose grandes diferencias economicas a expensas de una gran presion sobre los recursos forestales, que no propician una mejora en la formacion del individuo; se suma a ello el impuesto a la tierra,entre otros aspectos del marco regulatorio de la actividad. La investigacion realizada permitio identificar vacios teorico-metodologicos, y contradicciones que existen respecto a la gestion forestalen Esmeraldas para enfrentar y resolver los problemas sociales existentes en la region. Palabras clave:bosques ecuatoriales, economia local, juntas parroquiales
Accurate Three-Dimensional Reconstruction of Intravascular Ultrasound Data (1996)

These preliminary data support the use of this new method of 3D reconstruction of vascular structures with use of combined vascular ultrasound data and simultaneous ECG-gated biplane cinefluoroscopy.
[Serum superoxide dismutase (SOD) activity in diabetes mellitus]. (1993)

The serum superoxide dismutase (s-SOD) activities in patients with diabetes mellitus (DM) were assayed in order to evaluate its usefulness for monitoring of DM and also evaluate the relation between s-SOD activities and microangiopathies (nephropathy, neuropathy and retinopathy). As results followings were obtained; 1) s-SOD activities in DM patients were significantly higher than those in healthy controls (12.56 +/- 7.73 vs 10.51 +/- 1.69, p < 0.01). 2) There was no relations between s-SOD activities and FBS-, fructosamine- and HbA1-levels, respectively. 3) Among DM patients s-SOD activities were significantly higher in patients with microangiopathy than those in patients without microangiopathy (14.18 +/- 11.00 vs 11.24 +/- 3.13, p < 0.01). 4) Among DM patients with microangiopathy higher s-SOD activities tended to be observed in patients with triopathy such as nephropathy, neuropathy and retinopathy than in those with one or two microangiopathic complications. 5) Among DM patients with nephropathy the correlation was present between s-SOD activities and levels of creatinine. These results suggest that the assay of s-SOD activity is not useful for the monitoring of DM, however, it is suggested that the high s-SOD activity reflects the microangiopathic complications, particularly nephropathy.
Direct sulfation of calcium carbonate: the influence of carbon dioxide partial pressure (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDirect sulfation of calcium carbonate: the influence of carbon dioxide partial pressureClaes Tullin, Gunnar Nyman, and Sohbat GhardashkhaniCite this: Energy Fuels 1993, 7, 4, 512–519Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/ef00040a012https://doi.org/10.1021/ef00040a012research-articleACS PublicationsRequest reuse permissionsArticle Views251Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The role of the CFP2C9-Leu 359 allelic variant in the tolbutamide polymorphism (1996)

Tolbutamide undergoes hydroxylation in humans via a cytochrome P450-mediated pathway. The primary P450 isozyme responsible for this metabolism is thought to be CYP2C9. Population studies have indicated the existence of slow metabolizers of tolbutamide (approximately 1 in 500) suggesting a rare polymorphism associated with 2C9. Several allelic variants of 2C9 have been identified; however, the effect of these allelic variations on metabolism in vivo is not established. In the present study, the coding regions, intron-exon junctions, and upstream region of CYP2C9 were amplified by PCR and sequenced in two slow metabolizers. One individual was homozygous for Leu359/Leu359 and the other individual was heterozygous for Arg144/Cys144 and for Ile359/Leu359. No other genetic variations in 2C9 were detected in these individuals. PCR-RFLP tests showed that Arg144 Tyr358 Ile359 Gly417 is the principle CYP2C9 allele. Frequencies of the rarer Leu359 and Cys144 alleles were 0.06 and 0.08, respectively, in a Caucasian-American population and 0.005 and 0.01 respectively in African-Americans. The frequency of the Leu359 allele was 0.026 in Chinese-Taiwanese, but the Cys144 allele was not detected in this population. Studies in a recombinant yeast expression system showed that the Leu359 variant had the highest Km and the lowest Vmac for hydroxylation of tolbutamide of all the CYP2C9 allelic variants. This allelic variant also had the highest Km for the 7-hydroxylation of S-warfarin. The present data suggest that the incidence of the Leu359 allelic variant of CYP2C9 may account for the occurrence of poor metabolizers of tolbutamide.
Soft lithography and microfabrication (1998)

Miniaturization is a central theme in technology. As the computer industry knows, smaller microelectronic devices have made computers faster, cheaper and more portable. These advances in microelectronics have also spawned a huge variety of other tiny devices. For example, micro-electro-mechanical systems are now being used in medicine as disposable blood-pressure sensors, and in the automotive industry as tiny accelerometers in airbags that protect drivers in crashes.
Quantum Walks of Correlated Photons (2010)

Quantum walks of correlated particles offer the possibility of studying large-scale quantum interference; simulating biological, chemical, and physical systems; and providing a route to universal quantum computation. We have demonstrated quantum walks of two identical photons in an array of 21 continuously evanescently coupled waveguides in a SiO(x)N(y) chip. We observed quantum correlations, violating a classical limit by 76 standard deviations, and found that the correlations depended critically on the input state of the quantum walk. These results present a powerful approach to achieving quantum walks with correlated particles to encode information in an exponentially larger state space.
Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells (1987)

Two monoclonal antibodies (To15 and 4KB128) specific for the B cell-associated CD22 antigen (135,000 mol wt) are described. On immunoenzymatic analysis of cryostat tissue sections, these antibodies strongly label both mantle zone and germinal center B lymphoid cells in secondary lymphoid follicles (and also scattered extrafollicular lymphoid cells) but are unreactive with other cell types (with the exception of weak reactivity with some epithelioid histiocytes). These reactions differ from those of monoclonal antibodies B1 and B2 (anti-CD20 and CD21) but are similar to those of the pan-B antibody B4 (anti-CD19). One of the anti-CD22 antibodies (To15) has been tested extensively by immunoenzymatic labeling on greater than 350 neoplastic lymphoid and hematological samples. The CD22 antigen was found in tissue sections in most B cell-derived neoplasms, the major exceptions being myeloma (all cases negative) and a small proportion of high-grade lymphoma (6% of cases negative). In cell smears, the antigen could be found on neoplastic cells in most B cell lymphoproliferative disorders, including common acute lymphoblastic leukemia (ALL) (90% positive) and B cell chronic lymphocytic leukemia (CLL) (89% positive). We conclude that anti-CD22 antibodies are of value for identification of human B cell lymphoproliferative disorders (especially when used in conjunction with anti-CD19 antibodies). Previous reports that the CD22 antigen is absent from many B cell neoplasms are probably due to its being expressed within the cytoplasm of immature B cells rather than on their surface.
Molecular mechanisms of CRISPR-mediated microbial immunity (2013)

Bacteriophages (phages) infect bacteria in order to replicate and burst out of the host, killing the cell, when reproduction is completed. Thus, from a bacterial perspective, phages pose a persistent lethal threat to bacterial populations. Not surprisingly, bacteria evolved multiple defense barriers to interfere with nearly every step of phage life cycles. Phages respond to this selection pressure by counter-evolving their genomes to evade bacterial resistance. The antagonistic interaction between bacteria and rapidly diversifying viruses promotes the evolution and dissemination of bacteriophage-resistance mechanisms in bacteria. Recently, an adaptive microbial immune system, named clustered regularly interspaced short palindromic repeats (CRISPR) and which provides acquired immunity against viruses and plasmids, has been identified. Unlike the restriction–modification anti-phage barrier that subjects to cleavage any foreign DNA lacking a protective methyl-tag in the target site, the CRISPR–Cas systems are invader-specific, adaptive, and heritable. In this review, we focus on the molecular mechanisms of interference/immunity provided by different CRISPR–Cas systems.
Cytotoxic activity of methanolic extract and two alkaloids extracted from seeds of Peganum harmala L. (2005)

Objective: To study the cytotoxic activity of P. harmala . Materials and method: The alkaloids harmine and harmaline have been isolated from a methanolic extract from the seeds of P. harmala L. and have been characterized by spectroscopic-Mass and NMR methods. The cytotoxicity of the methanolic extract and both alkaloids has been investigated in the three human cancer cell lines UACC-62 (melanoma), TK- 10 (renal) and MCF-7 (breast) and then compared to the positive control effect of the etoposide. Results and conclusion: The methanolic extract and both alkaloids have inhibited the growth of these three cancer cell-lines and we have discussed possible mechanisms involved in their cytotoxicity.
Privacy-Preserving Logistic Regression with Distributed Data Sources via Homomorphic Encryption (2016)

Logistic regression is a powerful machine learning tool to classify data. When dealing with sensitive or private data, cares are necessary. In this paper, we propose a secure system for privacy-protecting both the training and predicting data in logistic regression via homomorphic encryption. Perhaps surprisingly, despite the non-polynomial tasks of training and predicting in logistic regression, we show that only additively homomorphic encryption is needed to build our system. Indeed, we instantiate our system with Paillier, LWE-based, and ring-LWE-based encryption schemes, highlighting the merits and demerits of each instantiation. Besides examining the costs of computation and communication, we carefully test our system over real datasets to demonstrate its utility.
The Radioisotope Contributes Significantly to the Activity of Radioimmunotherapy (2004)

Unlabeled Tositumomab showed single agent activity, but in this direct comparison, all of the therapeutic outcome measures were significantly enhanced by the conjugation of (131)I to Tositumomab.
Towards integrated microliquid handling systems (1994)

In this paper we describe components for integrated microliquid handling systems such as fluid injection analysis, and first results of planar integration of components. The components discussed are channels, passive and active valves, actuators for micropumps, micromixers, microflow sensors, optical detectors, pumps and dosage systems. The dosage system described comprises a flow sensor and a pump micromachined on a single silicon wafer sandwiched between Pyrex wafers. The liquid pump is of the reciprocating type with a thermo-pneumatic actuator. The microliquid flow sensor is based on the thermal anemometer type. Both pump and flow sensor are realized in a 3 inch (100)- Si wafer using a KOH bulk etch from both sides of the wafer. The dosing system allows accurate dosing of liquid in the wl regime and can easily be integrated with components as mixers and detectors to microliquid handling systems. A new concept for micromixing of liquids is introduced and its feasibility is demonstrated. The mixer allows fast mixing of small amounts of two liquids and it is applicable to microliquid handling systems. The,mixer has a channel for the liquid, an inlet port for the reagent, and a mixing area, the bottom of which has 400 micronozzles (15 pm xi5 Nm). Through these nozzles, a reagent is injected into the sample liquid, making many microplumes. These plumes speed up mixing by diffusion over a short distance
Conditional transformation of a pancreatic beta-cell line derived from transgenic mice expressing a tetracycline-regulated oncogene. (1995)

Conditional oncogene expression in transgenic mice is of interest for studying the oncoprotein requirements during tumorigenesis and for deriving cell lines that can be induced to undergo growth arrest and enhance their differentiated functions. We utilized the bacterial tetracycline (Tet)-resistance operon regulatory system (tet) from Tn10 of Escherichia coli to control simian virus 40 (SV40) large tumor (T) antigen (TAg) gene expression and to generate conditionally transformed pancreatic beta cells in transgenic mice. A fusion protein containing the tet repressor (tetR) and the activating domain of the herpes simplex virus protein VP16, which converts the repressor into a transcription activator, was produced in beta cells of transgenic mice under control of the insulin promoter. In a separate lineage of transgenic mice, the TAg gene was introduced under control of a tandem array of tet operator sequences and a minimal promoter, which by itself is not sufficient for gene expression. Mice from the two lineages were then crossed to generate double-transgenic mice. Expression of the tetR fusion protein in beta cells activated TAg transcription, resulting in the development of beta-cell tumors. Tumors arising in the absence of Tet were cultured to derive a stable beta-cell line. Cell incubation in the presence of Tet led to inhibition of proliferation, as shown by decreased BrdUrd and [3H]thymidine incorporation. The Tet derivative anhydrotetracycline showed a 100-fold stronger inhibition compared with Tet. When administered in vivo, Tet efficiently inhibited beta-cell proliferation. These findings indicate that transformed beta cells selected for growth during a tumorigenesis process in vivo maintain a dependence on the continuous presence of the TAg oncoprotein for their proliferation. This system provides an approach for generation of beta-cell lines for cell therapy of diabetes as well as conditionally transformed cell lines from other cell types of interest.
High-speed ultrasound volumetric imaging system. I. Transducer design and beam steering (1991)

Transducer design and phased array beam steering are developed for a volumetric ultrasound scanner that enables the 3-D visualization of dynamic structures in real time. The authors describe the design considerations and preliminary evaluation of a high-speed, online volumetric ultrasound imaging system that uses the principles of pulse-echo, phased array scanning with a 2-D array transducer. Several 2-D array designs are analyzed for resolution and main lobe-side lobe ratio by simulation using 2-D fast Fourier transform methods. Fabrication techniques are described for 2-D array transducer. Experimental measurements of pulse-echo point spread responses for 2-D arrays agree with the simulations. Measurements of pulse-echo sensitivity, bandwidth, and crosstalk are included.
The Autotrophic Pathway of Acetate Synthesis in Acetogenic Bacteria (1986)

Human coronavirus (HCoV) infection causes respiratory diseases with mild to severe outcomes. In the last 15 years, we have witnessed the emergence of two zoonotic, highly pathogenic HCoVs: severe acute respiratory syndrome coronavirus (SARS-CoV) and ...Read More
Formation of Cartilage In Vivo with Immobilized Autologous Rabbit Auricular Cultured Chondrocytes in Collagen Matrices (2007)

The authors suggest that this approach is of value for future clinical use.
Structure and formation of microplasmin. (1987)

The structure of human microplasmin, prepared from plasmin in alkaline solution, has been studied. Microplasmin consists of two polypeptide chains connected by disulfide bonds. One polypeptide is the B chain of plasmin consisting of 230 amino acids, and the other peptide is the COOH-terminal portion of the A chain of plasmin consisting of 31 amino acid residues. Microplasmin has a molecular weight of 28,635, calculated from its primary sequence. It is slightly more positively charged than plasminogen and is a more hydrophobic molecule. The proposed scheme for the formation of microplasmin involves autolysis at specific peptide bonds and scrambling of especially sensitive disulfide bonds in alkaline solution.
The human peroxisomal ABC half transporter ALDP functions as a homodimer and accepts acyl–CoA esters (2008)

Peroxisomes play a major role in human cellular lipid metabolism, including the beta-oxidation of fatty acids. The most frequent peroxisomal disorder is X-linked adrenoleukodystrophy (X-ALD), which is caused by mutations in the ABCD1 gene. The protein involved, called ABCD1, or alternatively ALDP, is a member of the ATP-binding-cassette (ABC) transporter family and is located in the peroxisomal membrane. The biochemical hallmark of X-ALD is the accumulation of very long-chain fatty acids (VLCFAs), due to an impaired peroxisomal beta-oxidation. Although this suggests a role of ALDP in VLCFA import, no experimental evidence is available to substantiate this. In the yeast Saccharomyces cerevisiae, peroxisomes are the exclusive site of fatty acid beta-oxidation. Earlier work has shown that uptake of fatty acids into peroxisomes may occur via two routes, either as free fatty acids thus requiring intraperoxisomal activation into acyl-CoA esters or as long-chain acyl-CoA esters. The latter route involves the two peroxisomal half ABC transporters Pxa1p and Pxa2p that form a heterodimeric complex in the peroxisomal membrane. Using different strategies, including the analysis of intracellular acyl-CoA esters by tandem-MS, we show that the Pxa1p/Pxa2p heterodimer is involved in the transport of a spectrum of acyl-CoA esters. Interestingly, we found that the mutant phenotype of the pxa1/pxa2Delta mutant can be rescued, at least partially, by the sole expression of the human ABCD1 cDNA coding for ALDP, the protein that is defective in the human disease X-linked adrenoleukodystrophy. Our data indicate that ALDP can function as a homodimer and is involved in the transport of acyl-CoA esters across the peroxisomal membrane.
Transcription Inhibition Using Oligonucleotide-Modified Gold Nanoparticles (2006)

The capture of T7 RNA polymerase using double-stranded promoter DNA on the surface of gold nanoparticles has been demonstrated. The competitive binding and inhibition of T7 RNA polymerase due to specific interactions on the nanoparticle surface represents a transcription factor decoy approach in a model system. The efficiency of inhibition was determined for various nanoparticle sizes, surface coverage, and linker length for double-stranded promoter DNA on gold nanoparticles. The experiments provide a basis for determining the accessibility of binding sites on nanoparticle surfaces for applications involving cell targeting or the use of nanoparticles as binding agents in solution.
Affinity purification of the hepatic high-density lipoprotein receptor identifies two acidic glycoproteins and enables further characterization of their binding properties (1992)

Several high-density lipoprotein (HDL)-binding proteins, candidates for the putative HDL receptor, have recently been identified, including two membrane proteins: HB1 of 120 kDa and HB2 of 100 kDa, present in rat and human liver plasma membranes respectively. Further insights into their function however, have been hampered by poor recoveries of these hydrophobic peptides, and the present work was undertaken to improve yields and enable a more detailed investigation of their properties. A significant improvement has been achieved using two affinity chromatographic procedures, one exploiting the glycoprotein nature of the proteins and the other exploiting their ligand properties, which in combination resulted in considerable enrichment of HB1 and HB2. Thus DEAE-Sephacel fractionation (0.05-0.2 M-NaCl) of CHAPS-solubilized plasma membranes yielded active HDL-binding proteins which bound to concanavalin A-Sepharose or wheat-germ-lectin-Sepharose columns and retained their binding activity after eluting with methyl-alpha-D-mannoside or N-acetylglucosamine respectively. These glycoproteins were further purified by affinity chromatography using apo-HDL-Sepharose columns. Final purification required preparative SDS/PAGE. Investigation of the carbohydrate moieties of the proteins using glycosidases and two-dimensional gel electrophoresis revealed pI values ranging from 4.6 to 4.9 and from 4.5 to 4.7 for HB1 and HB2 respectively, which after treatment with neuraminidase shifted towards basic pH (5.4-5.7 and 5.3-5.5 respectively). The molecular masses were decreased to 115 kDa and 95 kDa respectively, demonstrating that sialic acid residues contributed significantly to the negative charge of the glycosylated peptides. Treatment with the enzyme peptide N-glycosidase F (N-glycanase) resulted in a decrease in molecular mass of HB1 and HB2 to 105 kDa and 80 kDa respectively, but endo-alpha-N-acetylgalactosaminidase (O-glycanase) treatment was not effective. Neither neuraminidase nor N-glycanase treatment destroyed activity, suggesting that sialic acids or N-linked oligosaccharides are not important determinants of HDL binding. Digestion of plasma membranes with trypsin or Pronase resulted in a loss of activity of both HB1 and HB2 that was not influenced by prior treatment with neuraminidase, suggesting that sialic acid residues play no protective role against proteolytic cleavage of HDL receptor proteins.
Clinical characteristics and primary treatment of prostate cancer in Sweden between 1996 and 2005 (2007)

All changes in the register seen over time are consistent with increased diagnostic activity, especially PSA testing, resulting in an increased number of cases with early disease, predominantly tumors in category T1c. The patterns of diagnosis and treatment of prostate cancer vary considerably in different parts of Sweden. The NPCR continues to be an important source for research, epidemiological surveillance of the incidence, diagnosis and treatment of prostate cancer.