gebhart/patrepeval huggingface dataset:
Direct-Detection Optical OFDM Transmission System Without Frequency Guard Band (2010)

Interleaver and turbo code techniques are used in high spectral-efficient direct-detection optical orthogonal frequency-division-multiplexing (OFDM) transmission systems allowing significant improvement of optical receiver sensitivity by mitigating the unbalanced noise distribution in subcarrier signals. The optical signal-to-noise ratio of 5.18 Gb/s, 7.77-Gb/s coded OFDM (5.18 Gb/s after decoding), and 7.77-Gb/s coded OFDM with interleaver (5.18 Gb/s after decoding) over 200-km single-mode fiber at a bit-error rate of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-4</sup> are 23.7, 18.0, and 17.1 dB, respectively.
Interleukin 12 (IL-12) induces tyrosine phosphorylation of JAK2 and TYK2: differential use of Janus family tyrosine kinases by IL-2 and IL-12. (1995)

Interleukin (IL-12) has many effects on the function of natural killer and T cells, and is important in the control of cell-mediated immunity. IL-2 and IL-12 display many similar activities, yet each also induces a distinct set of responses. A human IL-12 receptor subunit has recently been cloned and, like the IL-2R beta and IL-2R gamma, is a member of the hematopoietic receptor superfamily; however, the molecular mechanisms of IL-12 action are unknown. In this report we show that IL-12 and IL-2 induce tyrosine phosphorylation of distinct members of the Janus (JAK) family of protein tyrosine kinases in human T lymphocytes. IL-12, but not IL-2, stimulates the tyrosine phosphorylation of TYK2 and JAK2, whereas JAK1 and JAK3, which are phosphorylated in response to IL-2, are not phosphorylated after IL-12 treatment. The use of distinct but related JAK family tyrosine kinases by IL-12 and IL-2 may provide a biochemical basis for their different biological activities.
Expression and secretion of the cloned Pseudomonas aeruginosa exotoxin A by Escherichia coli (1988)

The exotoxin A gene from Pseudomonas aeruginosa PAK was expressed in Escherichia coli from recombinant plasmids when transcription was initiated from a promoter in the cloning vector. The exotoxin A polypeptide synthesized was found to have an electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels of 66,000 daltons, identical in size to the mature exotoxin A made by P. aeruginosa. Analysis of the location of exotoxin A in various bacterial compartments by immunoblotting revealed that exotoxin A was exported by E. coli into its periplasmic space. Several functional assays, including analyses of disulfide bond formation, potentiation of ADP-ribosyltransferase activity, and HeLa cell cytotoxicity, were used to establish that the conformation of exotoxin A isolated from the E. coli periplasmic space is identical to that of exotoxin exported by P. aeruginosa to its extracellular space. Previous studies with recombinant plasmids expressing exotoxin A from P. aeruginosa PA103 (G. D. Gray, D. Smith, J. Baldridge, R. Markins, M. Vasil, E. Chen, and M. Heyneker, Proc. Natl. Acad. Sci. USA 81:2645-2649, 1984) showed a complete lack of processing and export of pre-exotoxin A in E. coli, differing from results reported here. These discrepancies may be explained by observed differences in the sequence of signal peptides encoded by the exotoxin A genes of PAK and PA103 strains of P. aeruginosa.
Chronopharmacology of antitumor effect induced by interferon-beta in tumor-bearing mice. (2000)

The mechanisms underlying the dosing time-dependent change in the antitumor effect of interferon-beta (IFN-beta) were investigated based on the sensitivity of tumor cells and the pharmacokinetics of the drug. Tumor-bearing mice were housed under standardized light-dark cycle conditions (lights on at 7:00 AM, off at 7:00 PM) with food and water available ad libitum. The antitumor effect of IFN-beta (0.5 MI.U./kg, intratumoral) was more efficient in early light phase than in early dark phase. The higher antitumor effect of IFN-beta was observed when specific binding of IFN receptor and DNA synthesis in tumor cells increased, and the lower effect was observed when these levels decreased. The dosing time-dependent effect of IFN-beta was supported by the time-dependent expression of transcription factor (signal transducers and activators of transcription 1) and cell proliferation inhibitor (p21 wild-type p53-activated fragment 1) protein induced by IFN-beta. There was a significant dosing time-dependent change in IFN-beta concentration in tumor, with a higher level in early light phase and a lower level in early dark phase. The dosing time-dependent change of IFN-beta concentration in tumor was associated with that of IFN-beta-induced antitumor effect. These results suggest that by choosing the most suitable dosing time for IFN-beta, the efficacy of the drug can be increased in certain experimental and clinical situations.
Subcutaneous trastuzumab: development of a new formulation for treatment of HER2-positive early breast cancer (2013)

Trastuzumab is a monoclonal antibody directed against the human epidermal growth factor receptor 2 (HER2). HER2 is amplified or overexpressed in about 15% of breast cancers and is associated with aggressive disease. Clinical benefits of trastuzumab have been established in the treatment of both early and metastatic HER2-positive breast cancer. Patients with HER2-positive early breast cancer have to be treated with trastuzumab for one year in combination with and sequentially after chemotherapy. This requires that trastuzumab is intravenously infused over 30-90 minutes every 3 weeks for one year which is time-consuming for both the patient and the health care provider. Consequently, a subcutaneous formulation of trastuzumab using a recombinant human hyaluronidase has been developed. Recombinant human hyaluronidase transiently increases absorption and dispersion in the subcutaneous space of large therapeutic proteins, such as monoclonal antibodies, allowing subcutaneous administration of trastuzumab in about 5 minutes. Thus, subcutaneous trastuzumab could represent a new treatment option that could have benefit to both the patient and the health care system. This review focuses on the development of the subcutaneous trastuzumab formulation and analyzes clinical trials assessing the pharmacokinetics, efficacy, and safety of this new formulation.
Purification and properties of an extracellular endoglucanase from Myceliophthora thermophila D-14 (ATCC 48104) (1990)

An extracellular endoglucanase (1,4-beta-glucanohydrolase, EC 3.2.1.4) produced by Myceliophthora thermophila D-14 (ATCC 48104) has been purified to homogeneity by ammonium sulphate precipitation and two consecutive ion-exchange chromatographic steps on DEAE-Sephadex A-50 columns. The enzyme was purified 13.8-fold and was homogeneous by analytical PAGE and SDS-PAGE. It has a high apparent Mr, of about 100,000. The pH and temperature optima for its activity were 4.8 and 65 degrees C respectively. The Km of the purified enzyme for CMC (sodium salt) was 3 mg ml-1. The enzyme displayed low activity toward salicin and p-nitrophenyl beta-D-glucoside. The activity was enhanced in the presence of Na+, K+ and Ca2+ but effectively inhibited by Hg2+, Fe2+, Mg2+, Cu2+ and NH4+. Inhibition studies indicated that the enzyme may be a metalloprotein and/or that it requires metal ions for its optimum activity.
Phylogenetic Analysis Using PHYLIP (2003)

Phylogenetic analysis is a powerful tool to study the relationships among sequences. From such relationships the origins, evolution, and possible changes in structural and functional properties of genes can be inferred.
Properties of a new alkaline proteinase from Aspergillus niger. (1989)

A. niger LCF 9 synthesizes a new aspergillopeptidase of potential interest in therapeutics. The properties and operating range of the enzyme were determined. It is a semi-alkaline aspergillopeptidase (EC 3.4.23.4) with one endopeptidase activity. Its pI is 4.10, its molecular weight is 21000 Da and its A1%(1 cm) at 280 nm is 9.75. It rapidly hydrolyzes casein and hemoglobin. Its optimal pH is 7.8 and optimal temperature is 45 degrees C. It is thermally labile above 40 degrees C but can be stabilized by adding calcium ions. It is inhibited by phenylmethylsulfonyl fluoride (PMSF), by ethylenediaminetetraacetic acid (EDTA) and by certain metals ions, e.g. copper, manganese and cobalt ions. It has no dipeptidase or tripeptidase activity and its esterase activity is weak. It has a high collagenase activity and is to our knowledge the only aspergillopeptidase that is active towards benzoyl-arginine p-nitroanilide (BAPNA).
Surface-Responsive Materials (2002)

Synthetic polymers offer a wealth of opportunities to design responsive materials triggered by external stimuli. Changing the length, chemical composition, architecture, and topology of the chains allows response mechanisms and rates to be easily manipulated; and devices based on the entropy of the chains, surface energies, and specific segmental interactions can readily be made. Although numerous applications exist, intriguing possibilities are emerging that have tremendous potential to further developments in surface-responsive materials.
Profane Intuitions: Kurdish Diaspora in the Turkish City (2001)

Contemporary analysis of the ‘Kurdish question’ in Turkey has often enough noted the recent explosion of Kurdish refugees and immigrants to the major Turkish cities. Interest in the re‐constitution of Kurdish identity in the metropolis, however, has been less evident. This article seeks to identify some of the main themes that need to be addressed in any such investigation. It begins by arguing that discourse on the ‘Kurdish question’ often frames the Kurds, rather than the Turkish state, as the problem. The response of the Turkish Republic to such a problem has been to assimilate the Kurds. The struggle against assimilation then is the common theme binding the multiple visions and experiences of the Kurdish diaspora.
Photon Counting (1968)

The fundamentals of photon counting using photomultipliers are described, including criteria for selecting suitable photomultipliers, some of the precautions that must be taken in using these devices, and methods of calculating the counting errors that may occur under various conditions of measurement. Problems of determining the time distribution of photons and, in particular, the coincident emission of photons which may be encountered in lasers and other simulated emission sources are also discussed. The question of photon counting with photoconductors is reviewed, and it is shown that it is extremely difficult, if not impossible, to achieve photon counting with simple photoconductors. However, carrier multiplication with photoconductive multipliers should eventually make possible photon counting with photoconductors. Photoconductive multipliers in one form or another have high quantum efficiency and wide spectral response, and will almost inevitably replace photomultipliers for photon counting.
Apodized surface-corrugated gratings with varying duty cycles (2000)

We present the first realization of a Bragg grating apodization based on a concatenation of different duty cycles. The gratings were fabricated in SiON planar waveguides. The reflected signal exhibits a sidelobe suppression better than -20 dB outside a bandwidth of 1.36 nm. At the same time the reflection is stronger than 99.9%, i.e., the resonant light is suppressed better than -30 dB in transmission over 0.22 nm. The gratings are perfectly suited to serve as the wavelength-selective element of a Mach-Zehnder add/drop filter.
Retrieval of sketches based on spatial relation between strokes (2003)

Proposes a method to retrieve sketches stored in the form of multiple strokes, by extracting the shape information for each stroke and by considering the spatial relation between the strokes. The spatial relation between the strokes can be used to determine the stroke correspondence between the query sketch and the database sketch. Moreover, the similarity between the relationship among query strokes and database strokes can be used as part of the matching score. Sketch retrieval is useful in various applications, such as retrieval of free-form hand-drawings, hand-written or printed text, and trademarks. For freeform hand-drawings, such as lecture notes captured digitally from a whiteboard, a user can easily search through a database of hand-drawings by inputting a sketch about what he/she is looking for, without the trouble of describing it using keywords. For hand-written or printed text, such as a protest sign in a video scene, sketch retrieval can be used to recognize the text without the need to first identify each character. For trademarks, such as company logos, sketch retrieval is useful in adding automation to the feature extraction and the retrieval process.
A murine model of neurofibromatosis type 2 that accurately phenocopies human schwannoma formation (2014)

Neurofibromatosis type 2 (NF2) is an autosomal dominant genetic disorder resulting from germline mutations in the NF2 gene. Bilateral vestibular schwannomas, tumors on cranial nerve VIII, are pathognomonic for NF2 disease. Furthermore, schwannomas also commonly develop in other cranial nerves, dorsal root ganglia and peripheral nerves. These tumors are a major cause of morbidity and mortality, and medical therapies to treat them are limited. Animal models that accurately recapitulate the full anatomical spectrum of human NF2-related schwannomas, including the characteristic functional deficits in hearing and balance associated with cranial nerve VIII tumors, would allow systematic evaluation of experimental therapeutics prior to clinical use. Here, we present a genetically engineered NF2 mouse model generated through excision of the Nf2 gene driven by Cre expression under control of a tissue-restricted 3.9kbPeriostin promoter element. By 10 months of age, 100% of Postn-Cre; Nf2(flox/flox) mice develop spinal, peripheral and cranial nerve tumors histologically identical to human schwannomas. In addition, the development of cranial nerve VIII tumors correlates with functional impairments in hearing and balance, as measured by auditory brainstem response and vestibular testing. Overall, the Postn-Cre; Nf2(flox/flox) tumor model provides a novel tool for future mechanistic and therapeutic studies of NF2-associated schwannomas.
APC binds to the novel protein EB1. (1995)

Mutations of the APC gene play a critical role in both sporadic and familial forms of colorectal cancer. The vast majority of these mutations result in the loss of the carboxyl terminus of the protein. To further elucidate the function of APC, we searched for cellular proteins that associate with its carboxyl terminus. One million human cDNA clones were screened with the use of the interaction trap two-hybrid system, and 67 clones were found to have a phenotype suggestive of an APC-interacting protein. Nucleotide sequence analysis revealed that 48 of these clones were derived from a single novel named EBI. The association of APC and EB1 proteins was confirmed with in vitro binding assays. mAbs against EB1 were then produced and used to demonstrate the association of APC and EB1 in vivo. The EB1 gene was predicted to encode a 268-amino acid protein without significant homology to proteins with known function. However, searches of nucleotide databases did identify evidence for at least two related human genes and a yeast homologue. This conservation suggests an essential function for EB1 that might provide clues to the mechanism through which APC suppresses colonic neoplasia.
Botulinum toxin type A treatment in neurogenetic syndromes (2005)

The results suggest that obtaining family input may be useful when treating spasticity in unusual circumstances. The use of BTX-A in uncommon neurogenetic syndromes was supported by the majority of families interviewed.
Skin Arthroplasty of the Hip (1961)

The rationale of interposition of skin in arthroplasty was examined. Sixty operations were performed on thirty dogs; of these, fifty-three were arthroplastic operations, with implantation of fresh autogenous skin, or a part of it, as an interposing membrane. Six hip joints became dislocated postoperatively, leaving forty-seven technically successful operations. The animals were observed for periods of one week to twenty-four months. It was found that skin membranes did not survive within the hip joints of dogs or contribute to formation of new articular surfaces. Progressive formation of a new joint surface was observed in most animals. This consisted of tissue that had the morphological characteristics of fibrocartilage. Its formation occurred similarly with or without implantation of full-thickness skin or portions of skin. It is suggested that the newly formed joint surface in these animals is derived from immature connective tissue, probably of local origin, and is dependent for its formation on the compressive force of the joint environment and on an adequate blood supply of the underlying bone.
Potential mechanisms of human natural killer cell expansion in vivo during low-dose IL-2 therapy (2000)

The continuous, in vivo infusion of low-dose IL-2 selectively expands the absolute number of human natural killer (NK) cells after 4-6 weeks of therapy. The mechanism responsible for this expansion is unknown and was examined in this study. NK cells cultured at low concentrations of IL-2, comparable to those found during in vivo therapy, proliferate for 6 days and then exit the cell cycle. However, NK cells in vivo did not traverse the S/G(2)/M phase of the cell cycle during low-dose IL-2 therapy. Low concentrations of IL-2 delay programmed cell death of NK cells but have the same effect on resting T cells that do not expand in vivo. When CD34(+) bone marrow hematopoietic progenitor cells are cultured for 21 days with low concentrations of IL-2, they differentiate into CD56(+)CD3(-) NK cells, not T cells. Thus, the selective expansion of human NK cells during continuous in vivo infusion of low-dose IL-2 likely results from enhanced NK-cell differentiation from bone marrow progenitors, combined with an IL-2-dependent delay in NK-cell death, rather than proliferation of mature NK cells in the periphery.
Characterization of CMRF-44, a novel monoclonal antibody to an activation antigen expressed by the allostimulatory cells within peripheral blood, including dendritic cells. (1994)

Dendritic cells (DC) are the most potent antigen-presenting cells (APC) involved in the initiation of primary T-lymphocyte responses. However, despite their importance, no DC-specific surface marker has been identified in humans and many aspects of their ontogeny and the mechanisms underlying their potent functional activity remain unknown. In this report we describe a novel monoclonal antibody (mAb), CMRF-44, which recognizes an early activation antigen expressed by human DC and acts as a marker of allostimulatory activity within preparations of peripheral blood mononuclear cells (PBMC). The CMRF-44 antigen was expressed strongly on DC isolated from blood and tonsil by standard techniques, but was not detectable on Langerhans' cells within skin or on DC isolated directly from blood using a cell-sorting method which involves minimal DC manipulation/activation. Normal resting peripheral blood leucocytes did not label with CMRF-44, although weak staining of a small subpopulation (15%) of blood B lymphocytes was identified by double labelling. However, following overnight culture at 37 degrees, moderate staining of a subpopulation of PBMC was detected. Confirmation that CMRF-44 recognized an early marker of activated DC and hence allostimulatory activity was obtained by sorting cultured cell preparations on the basis of CMRF-44 reactivity. A marked enrichment of allostimulatory activity was observed in the CMRF-44-positive cellular population, whereas the CMRF-44-negative cells showed only minimal stimulatory activity. Activation studies established that the CMRF-44 antigen was an early activation marker, expressed constitutively on the majority of tonsil B lymphocytes, which can be induced on peripheral blood B lymphocytes and subpopulations of monocytes. Expression of the CMRF-44 antigen on cell lines was similarly restricted, CMRF-44 antigen being detected only on Hodgkin's disease-derived and B-lymphoid lines. The cellular distribution, expression kinetics and biochemical characteristics of the CMRF-44 antigen identify it as a new early marker of activated allostimulatory (DC) populations.
Increased IL-6 production and IL-6-mediated Ig secretion in murine host-vs-graft disease (1993)

BALB/c mice neonatally injected with semiallogenic (A/J x BALB/c)F1 splenocytes develop a host-vs-graft (HVG) reaction between host T cells and donor B cells, resulting in hypergammaglobulinemia, splenomegaly, and increased serum levels of various autoantibodies. This syndrome is associated with a polyclonal activation of the donor-derived B cells. High serum levels of IL-6 were found in 4-wk-old mice undergoing HVG disease (mean +/- SEM, 132 +/- 93 as compared with 12 +/- 2 in control mice, p < 0.05). Also supernatants of spleen cell cultures from HVG mice contained increased levels of IL-6. In situ hybridization and cell depletion experiments demonstrated that host macrophages were responsible for this pathologic IL-6 secretion. The spontaneous in vitro production of autoreactive antibodies by donor B cells from HVG mice was further enhanced by adding human rIL-6, whereas addition of human rIL-1 beta, human rIL-2, murine rIL-4, murine rIL-5, or combinations of these cytokines had no effect. Finally, addition of blocking anti-IL-6 and anti-IL-6 receptor mAb markedly reduced hyper IgG1 production in cultures of spleen cells from HVG mice. These data suggest that an increased production of IL-6 by persistently stimulated host macrophages is involved in the activation of donor B cells leading to HVG disease.
Quasi-optical techniques (1992)

The basic theory of quasi-optical Gaussian beam propagation and beam transformation by simple optical elements is summarized, and coupling to and between Gaussian beams is briefly discussed. Guidelines for Gaussian optics system design are reviewed, the most important being beam truncation and matching. Passive components in the terahertz frequency range based on quasi-optical propagation, including polarization processors, filters, diplexers, and ferrite devices, are examined. Some active quasi-optical devices, including multielement oscillators, frequency multipliers, and phase shifters, are described. Some specific applications of quasi-optical systems are briefly described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Nutritional Factors Affecting the Ratio of Solvents Produced by Clostridium acetobutylicum (1986)

Fermentation of whey by Clostridium acetobutylicum yielded butanol and acetone in a ratio of approximately 100:1. This ratio amounted to only 2:1 in synthetic media with glucose, lactose, or glucose plus galactose as substrates. Removal of citrate from whey and addition of minerals resulted in an increase in the amount of acetone produced. Experiments carried out in a chemostat with a low-phosphate synthetic medium revealed that the butanol/acetone ratio could be increased from 2:1 to 3.8:1 by cofermentation of l-lactate and from 2:1 to 8:1 by iron limitation. The performance of the fermentation in a low-iron glucose medium above pH 5.1 yielded l-lactate as the main product.