gebhart/patrepeval huggingface dataset:
Effects of Calsporin® on Turkey Performance, Carcass Yield and Nitrogen Reduction (2003)

Calsporin®, a probiotic that contains a strain of Bacillus subtilus in a spore form, has been used to increase performance of several species. An experiment was conducted to determine the effects of Calsporin® addition on gain, feed conversion, nitrogen excretion, and salmonella spp. count from the intestinal tract compared to a commercial antibiotic (Bacitracin-Zinc) in male turkeys to market age. Six hundred Hybrid large white tom poults were purchased from a commercial hatchery at day of hatch and randomly allotted into 24 pens, blocked by treatment. Treatments consisted of Calsporin® added at 3 0 grams per ton of the diet , Bacitracin-zinc at 50 grams per ton or no additive. Birds were housed in an industry standard curtain-sided barn on used pine shavings. Standard corn, soybean meal, animal by-product diets where used for all treatments with nutrient levels based on the 1994 NRC requirements for turkeys. Individual bird weights and pen feed consumption where collected at 21, 42, 63, 84, 105, 126 days. Daily mortality was collected and feed:gain was adjusted to account for mortality. Litter samples where taken from ten random pens at day 63. At 126 days of age three toms per pen where selected based on average pen weight and processed the following day to determine carcass and parts yield. Data were analyzed by analysis of variance (ANOVA) with a randomized block design using the general linear model. By week 12 the treatments that contained either Calsporin® or Bacitracin-zinc had a significantly (p < 0.05) improved gain compared to the treatment without either of the products . For the 0 to 15 and 0 to 18 week phases the birds without additives gained significantly (p< 0.05) less weight compared to all other treatments. No differences in feed efficiency, yield or mortality were observed. Litter samples indicated a reduction in ammonia volitization in Calsporin® fed birds. These data would indicate that Calsporin® may be used successfully as an antibiotic replacement in market turkeys.
Differential regulation of two glucose transporters by chronic growth hormone treatment of cultured 3T3-F442A adipose cells. (1990)

New methods for the analysis of glucose transporters were used to analyze the molecular mechanisms involved in the insulin-antagonistic effects of growth hormone (GH), which is known as a diabetogenic hormone. The ability of GH to alter the number and mRNA levels of two different glucose transporters in cultured 3T3-F442A adipocytes was investigated using specific antibodies and cDNA probes. At concentrations of GH as low as 0.5 and 5 ng/ml and at incubation times as short as 4 h, GH decreased rates of 2-deoxyglucose uptake in 3T3-F442A adipocytes. 3-O-Methyl-D-glucose uptake was inhibited to an extent similar to that of 2-deoxyglucose uptake (60-80%) after a 24-h incubation with GH (500 ng/ml), indicating that GH inhibits glucose metabolism specifically at the step of glucose transport. To determine whether reduced rates of glucose transport might result from reduced numbers of glucose transporters, whole cell lysates were prepared from GH-treated cells and subjected to immunoblotting using antibodies that identify Glut 1 (HepG2/rat brain) and Glut 4 (muscle/adipose) transporters. GH caused a time- and dose-dependent decrease in the number of Glut 1 transporters in the cell. Northern and slot-blot analyses showed a GH-induced dose-dependent decrease in levels of Glut 1 mRNA. In contrast, levels of Glut 4 transporter and mRNA were unchanged by GH. These data suggest that GH regulates Glut 1 and Glut 4 transporters differentially and that it exerts its inhibitory effect on glucose uptake at least in part by decreasing the synthesis of Glut 1 transporters. These studies provide the first evidence that GH regulates a key gene in metabolic regulation and can interfere with gene expression.
Differential transforming abilities of non-secreted and secreted forms of human fibroblast growth factor-1. (1993)

Fibroblast growth factor (FGF)-1(1-154), the precursor for acidic FGF-1(21-154), is a potent angiogenic polypeptide, the structure of which lacks a signal peptide sequence for secretion. To investigate the biological significance of this structural feature, we have attempted forced secretion of FGF-1 through fusion of the entire FGF-1 coding frame with the signal peptide (sp) from the hst/KS3 gene, a secretory member of the heparin-binding growth factor family. We also studied the transforming ability of the signal-less forms of FGF-1 comprising FGF(1-154) and FGF-1(21-154). The presence of a soluble and biologically active form of FGF-1 was readily detected in the conditioned medium of NIH 3T3 cells transfected with sp-hst/KS3:FGF-1(1-154) as demonstrated by Western blot analysis and DNA synthesis assays, whereas sp-hst/KS3:FGF-1(21-154) was not detectable in conditioned medium even though the protein was detected in cellular extracts. The secreted form of sp-hst/KS3:FGF-1(1-154) stimulated the proliferation of human umbilical vein endothelial cells in vitro and was able to induce receptor-mediated tyrosine phosphorylation. Furthermore, the forced secretion of biologically active FGF-1 resulted in NIH 3T3 cell transformation as demonstrated by altered morphology in vitro, the formation of discrete colonies in soft agarose, growth under serum-free conditions, and ability to rapidly form highly vascular tumors in vivo. Interestingly, sp-hst/KS3:FGF-1(21-154) also mediated the transition to a transformed phenotype despite the inability to detect extracellular FGF-1 in the media conditioned by these NIH 3T3 cell transfectants. Although the transfection of FGF-1(21-154) yielded similar NIH 3T3 cell morphologic changes, these transfectants did not grow under serum-free conditions or yield colonies in soft agarose, and formed tumors in vivo with delayed kinetics. Furthermore, the FGF-1(1-154) NIH 3T3 cell transfectants did not exhibit morphologic changes, and this may be due to the inability of mRNA to express protein. These data suggest that although non-sp forms of FGF-1 may alter the monolayer phenotype of NIH 3T3 cells in vitro, the ability of FGF-1 to transform NIH 3T3 cells requires the function of a sp-directed secretory pathway and suggests that this pathway increases tumorigenicity in vivo.
Lessons from a large outbreak of Escherichia coli O157[ratio ]H7 infections: insights into the infectious dose and method of widespread contamination of hamburger patties (1999)

Between November 1992 and February 1993, a large outbreak of Escherichia coli O157:H7 infections occurred in the western USA and was associated with eating ground beef patties at restaurants of one fast-food chain. Restaurants that were epidemiologically linked with cases served patties produced on two consecutive dates; cultures of recalled ground beef patties produced on those dates yielded E. coli O157:H7 strains indistinguishable from those isolated from patients, confirming the vehicle of illness. Seventy-six ground beef patty samples were cultured quantitatively for E. coli O157:H7. The median most probable number of organisms was 1.5 per gram (range, < 0.3-15) or 67.5 organisms per patty (range, < 13.5-675). Correlation of the presence of E. coli O157:H7 with other bacterial indicators yielded a significant association between coliform count and the presence of E. coli O157:H7 (P = 0.04). A meat traceback to investigate possible sources of contamination revealed cattle were probably initially colonized with E. coli O157:H7, and that their slaughter caused surface contamination of meat, which once combined with meat from other sources, resulted in a large number of contaminated ground beef patties. Microbiological testing of meat from lots consumed by persons who became ill was suggestive of an infectious dose for E. coli O157:H7 of fewer than 700 organisms. These findings present a strong argument for enforcing zero tolerance for this organism in processed food and for markedly decreasing contamination of raw ground beef. Process controls that incorporate microbiological testing of meat may assist these efforts.
Source of the Ice-Binding Specificity of Antifreeze Protein Type I (2000)

Antifreeze proteins (AFPs) are a group of structurally very diverse proteins with the unique capability of binding to the surface of seed ice crystals and inhibiting ice crystal growth. The AFPs bind with high affinity to specific planes of the ice crystal. Previously, this affinity of AFPs has been ascribed to the formation of multiple hydrogen bonds across the protein-ice interface, but more recently van der Waals interactions have been suggested to be the dominant energetic factors for the adsorption. To determine whether van der Waals interactions are also responsible for the binding specificities of AFPs, the protein-ice interaction of the helical AFP Type I from winter flounder (HPLC6) was studied using a Monte Carlo rigid body docking approach. HPLC6 binds in the [1102] direction of the [2021] plane, with the Thr-Ala-Asn surface comprising the protein's binding face. The binding of HPLC6 to this ice plane is highly preferred, but the protein is also found to bind favorably to the [1010] prism plane using a different protein surface comprised of Thr and Ala residues. The results show that van der Waals interactions, despite accounting for most of the intermolecular energy (>80%), are not sufficient to completely explain the AFP binding specificity.
A strategy for the generation of non‐aggregating mutants of Anthozoa fluorescent proteins (2001)

Recently, we cloned several fluorescent proteins of different colors homologous to Aequorea victoria green fluorescent protein, which have great biotechnological potential as in vivo markers of gene expression. However, later investigations revealed severe drawbacks in the use of novel fluorescent proteins (FPs), in particular, the formation of tetramers (tetramerization) and high molecular weight aggregates (aggregation). In this report, we employ a mutagenic approach to resolve the problem of aggregation. The elimination of basic residues located near the N-termini of FPs results in the generation of non-aggregating versions of several FPs, specifically, drFP583 (DsRed), DsRed-Timer, ds/drFP616, zFP506, zFP538, amFP486, and asFP595.
Theory of random population for quantum dots (1997)

Carrier capture and recombination in quantum dots are random processes. Conventional rate equation models do not take into account this property. Based on our theory of random population we predict recombination spectra, transients, and gain of quantum-dot ensembles. Even with infinitely fast interlevel energy relaxation excited levels become considerably populated. The impact of a slowdown of energy relaxation is modeled and criteria for a conclusive experimental observation of a finite interlevel-scattering time are given.
Optimal design of structured nanospheres for ultrasharp light-scattering resonances as molecular imaging multilabels (2005)

Optical molecular imaging could potentially enable noninvasive high-resolution characterization and diagnosis of living tissue. The capability to image multiple molecular targets simultaneously is particularly important. Currently this task cannot be achieved using conventional optical contrast agents, due to their broad spectral responses (approximately 80 to 200 nm). Developments in research on semiconductor nanocrystals (quantum dots) provide one possible solution. We describe a different concept of multilabel molecular imaging that utilizes resonant light-scattering spectroscopy of multilayered nanospheres to achieve tunable ultrasharp resonance peaks with widths as narrow as 10 nm. Our theoretical study demonstrates that dozens of molecular targets can potentially be imaged simultaneously using this approach.
High Efficiency White Organic Light‐Emitting Devices Incorporating Yellow Phosphorescent Platinum(II) Complex and Composite Blue Host (2013)

Abstract A new class of charge neutral, strongly luminescent cyclometalated platinum(II) complexes supported by dianionic tetradentate ligand are synthesized. One of these platinum(II) complexes, Y‐Pt , displays a high photoluminescence quantum yield of 86% and electroluminescence efficacy ( η power ) of up to 52 lm W −1 , and is utilized as a yellow phosphorescent dopant in the fabrication of white organic light‐emitting devices (WOLEDs). WOLEDs based on conventional structures with yellow emission from Y‐Pt in combination with blue emission from bis (4,6‐difluorophenyl‐pyridinato‐ N , C 2 ′) (picolinate) iridium(III) (FIrpic) show a total η power of up to 31 lm W −1 . A two‐fold increase in η power by utilizing a modified WOLED structure comprising of a composite blue host is realized. With this modified device structure, the total η power and driving voltage at a luminance of 1000 cd m −2 can be improved to 61 lm W −1 and 7.5 V (i.e., 10 V for control devices). The performance improvement is attributed to an effectively broaden exciton formation‐recombination zone and alleviation of localized exciton accumulation within the FIrpic‐doped composite host for reduced triplet‐triplet annihilation, yielding blue light‐emission with enhanced intensity. The modified device structure can also adopt a higher concentration of Y‐Pt towards its optimal value, leading to WOLEDs with high efficiency.
Drying of Organic Solvents: Quantitative Evaluation of the Efficiency of Several Desiccants (2010)

Various commonly used organic solvents were dried with several different drying agents. A glovebox-bound coulometric Karl Fischer apparatus with a two-compartment measuring cell was used to determine the efficiency of the drying process. Recommendations are made relating to optimum drying agents/conditions that can be used to rapidly and reliably generate solvents with low residual water content by means of commonly available materials found in most synthesis laboratories. The practical method provides for safer handling and drying of solvents than methods calling for the use of reactive metals, metal hydrides, or solvent distillation.
Fibrinogen-Fibrin Conversion and Inhibition of Fibrinotysis* (1982)

The fibrin adhesion technique is the imitation of the last step of the coagulation system. Fibrinogen is converted by thrombin into fibrin and stabilized by factor XIII. Fibrin sticks to the tissue and the tissue is adapted by syneresis. Local application of aprotinin to the thrombin solution is necessary in order to inhibit premature lysis of the fibrin film. This technique is now used in some selected cases in man such as fixation of cartilage, tendon, sealing of colonic anastomoses and preclotting of vessel grafts. An excellent hemostyptic effect of the fibrin glue in combination with absorbable collagen tampons was found. This combination technique is now used to seal parenchymatous organs and to stop hemorrhage in patients with defective hemostasis particularly those undergoing open heart surgery.
Flexible dry surface-electrodes for ECG long-term monitoring (2007)

In this paper a new generation of dry electrodes for ECG-recording purposes is presented. These electrodes are flexible, biocompatible, biostable and perform a dry signal acquisition without the use of any electrolyte gel or adhesive. Thereby no skin preparation is needed. The material properties allow integration into textiles to form intelligent clothes for long-term monitoring duties. The electrodes can be washed together with the carrier textiles and provide a very good wearing comfort resulting in wide acceptance by the patients. The Department Medical Engineering and Neuroprosthetics of the Fraunhofer Institute for Biomedical Engineering developed these electrodes mainly to process the Electrocardiogram, however, in first tests they proved their capability even for other biosignal requirements like surface-EMG. In either case the signal quality of the dry electrodes is comparable to commercial Ag/AgCl gel-electrodes.
Proof of Finite Surface Code Threshold for Matching (2012)

The field of quantum computation currently lacks a formal proof of experimental feasibility. Qubits are fragile and sophisticated quantum error correction is required to achieve reliable quantum computation. The surface code is a promising quantum error correction code, requiring only a physically reasonable 2D lattice of qubits with nearest neighbor interactions. However, existing proofs that reliable quantum computation is possible using this code assume the ability to measure four-body operators and, despite making this difficult to realize assumption, require that the error rate of these operator measurements is less than 10(-9), an unphysically low target. High error rates have been proved tolerable only when assuming tunable interactions of strength and error rate independent of distance, which is also unphysical. In this work, given a 2D lattice of qubits with only nearest neighbor two-qubit gates, and single-qubit measurement, initialization, and unitary gates, all of which have error rate p, we prove that arbitrarily reliable quantum computation is possible provided p < 7.4 × 10(-4), a target that many experiments have already achieved. This closes a long-standing open problem, formally proving the experimental feasibility of quantum computation under physically reasonable assumptions.
Modeling the spread of active worms (2003)

Active worms spread in an automated fashion and can flood the Internet in a very short time. Modeling the spread of active worms can help us understand how active worms spread, and how we can monitor and defend against the propagation of worms effectively. In this paper, we present a mathematical model, referred to as the Analytical Active Worm Propagation (AAWP) model, which characterizes the propagation of worms that employ random scanning. We compare our model with the Epidemiological model and Weaver's simulator. Our results show that our model can characterize the spread of worms effectively. Taking the Code Red v2 worm as an example, we give a quantitative analysis for monitoring, detecting and defending against worms. Furthermore, we extend our AAWP model to understand the spread of worms that employ local subnet scanning. To the best of our knowledge, there is no model for the spread of a worm that employs the localized scanning strategy and we believe that this is the first attempt on understanding local subnet scanning quantitatively.
Growth-regulating functions of human papillomavirus early gene products in cervical cancer cells acting dominant over enhanced epidermal growth factor receptor expression. (1990)

Squamous cell carcinomas of the human anogenital tract are usually associated with infection of specific types of human papillomaviruses (HPV 16, 18, 31, 33, 35). The intracellular concentration of human papillomavirus early gene products E6 and E7 has been directly linked to the proliferative capacity of cervical cancer cells. Since the expression rate of epidermal growth factor receptor correlates to growth properties in squamous carcinoma cell lines, it has been presumed that human papillomavirus early genes influence cell growth via enhanced epidermal growth factor receptor expression. This hypothesis implies that growth regulation by epidermal growth factor receptor overexpression dominates over a growth-regulatory influence of human papillomavirus early gene products in squamous carcinoma cells. To test this hypothesis epidermal growth factor receptor expression was analyzed in various clones of the C4-1 cervical cancer cell line which, upon dexamethasone treatment, express either increased or decreased levels of human papillomavirus 18 early gene products. In C4-1 clones expressing reduced levels of viral E6/E7 gene products upon glucocorticoid treatment expression of epidermal growth factor receptor was the same as in those clones displaying increased levels of papillomavirus proteins under identical culture conditions. The growth rate of the cells correlated with the level of viral gene products rather than with the expression of epidermal growth factor receptor. These findings suggest that unregulated overexpression of epidermal growth factor receptor is not the dominant mechanism of growth control in papillomavirus-positive carcinoma cells. Other, yet unknown pathways associated with papillomavirus early genes are essentially involved in growth control mechanisms of human cervical cancer cells.
Behavioral Disinhibition Induced by Tryptophan Depletion in Nonalcoholic Young Men With Multigenerational Family Histories of Paternal Alcoholism (1999)

Low serotonin levels may be implicated in the high disinhibition or impulsivity observed in some individuals with a genetic vulnerability to alcohol abuse or dependence.
Purification, Characterization, and Prebiotic Properties of Pectic Oligosaccharides from Orange Peel Wastes (2014)

Pectic oligosaccharides (POS) were obtained by hydrothermal treatment of orange peel wastes (OPW) and purified by membrane filtration to yield a refined product containing 90 wt % of the target products. AraOS (DP 3-21), GalOS (DP 5-12), and OGalA (DP 2-12, with variable DM) were identified in POS mixtures, but long-chain products were also present. The prebiotic potential of the concentrate was assessed by in vitro fermentation using human fecal inocula. For comparative purposes, similar experiments were performed using orange pectin and commercial fructo-oligosaccharides (FOS) as substrates for fermentation. The dynamics of selected microbial populations was assessed by fluorescent in situ hybridization (FISH). Gas generation, pH, and short-chain fatty acid (SCFA) production were also measured. Under the tested conditions, all of the considered substrates were utilized by the microbiota, and fermentation resulted in increased numbers of all the bacterial groups, but the final profile of the microbial population depended on the considered carbon source. POS boosted particularly the numbers of bifidobacteria and lactobacilli, so that the ratio between the joint counts of both genera and the total cell number increased from 17% in the inocula to 27% upon fermentation. SCFA generation from POS fermentation was similar to that observed with FOS, but pectin fermentation resulted in reduced butyrate generation.
Poly(2‐oxazoline)s: A Polymer Class with Numerous Potential Applications (2009)

The living cationic ring-opening polymerization of 2-oxazolines has been studied in great detail since its discovery in 1966. The versatility of this living polymerization method allows copolymerization of a variety of 2-oxazoline monomers to give a range of tunable polymer properties that enable, for example, hydrophilic, hydrophobic, fluorophilic, as well as hard and soft materials to be obtained. However, this class of polymers was almost forgotten in the 1980s and 1990s because of their long reaction times and limited application possibilities. In the new millennium, a revival of poly(2-oxazoline)s has arisen because of their potential use as biomaterials and thermoresponsive materials, as well as the easy access to defined amphiphilic structures for (hierarchical) self-assembly. Recent developments that illustrate the potential of poly(2-oxazoline)s are discussed in this Review. In addition, the promising combination of poly(2-oxazoline)s and click chemistry is illustrated.