gebhart/patrepeval huggingface dataset:
Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge (2001)

Foreign glycoproteins expressed in recombinant vesicular stomatitis virus (VSV) can elicit specific and protective immunity in the mouse model. We have previously demonstrated the expression of respiratory syncytial virus (RSV) G (attachment) and F (fusion) glycoprotein genes in recombinant VSV. In this study, we demonstrate the expression of RSV F and G glycoproteins in attenuated, nonpropagating VSVs which lack the VSV G gene (VSVDeltaG) and the incorporation of these RSV proteins into recombinant virions. We also show that intranasal vaccination of mice with nondefective VSV recombinants expressing RSV G (VSV-RSV G) or RSV F (VSV-RSV F) elicited RSV-specific antibodies in serum (by enzyme-linked immunosorbent assay [ELISA]) as well as neutralizing antibodies to RSV and afford complete protection against RSV challenge. In contrast, VSVDeltaG-RSV F induced detectable serum antibodies to RSV by ELISA, but no detectable neutralizing antibodies, yet it still protected from RSV challenge. VSVDeltaG-RSV G failed to induce any detectable serum (by ELISA) or neutralizing antibodies and failed to protect from RSV challenge. The attenuated, nonpropagating VSVDeltaG-RSV F is a particularly attractive candidate for a live attenuated recombinant RSV vaccine.
Capillary isoelectric focusing‐mass spectrometry: Coupling strategies and applications (2015)

IEF on immobilized pH gradient strips is a widespread tool for protein separation, especially as first dimension in commonly utilized 2DE. In the latter arrangement, separations are based on two orthogonal molecular characteristics according to pI in the first and molecular weight in the second dimension. However, the approach is time consuming, quantification is difficult and MS can be applied only offline. Capillary IEF and related IEF techniques in combination with MS provide similar information. The major benefits are high mass resolution and mass accuracy, reproducibility, speed, automation, and quantification by using a high-resolution mass spectrometer. However, online hyphenation of CIEF with MS is interfered by the ampholytes, acids, and bases needed for high-resolution IEF. This review will give an overview about important coupling techniques, like low ampholyte concentration, interim separation by chromatography, or the use of a dialysis interface to separate the analytes from interfering substances. It is focused on strategies which allow sensitive MS detection of CIEF-separated analytes. In addition, proteomic and biopharmaceutical applications of capillary IEF techniques combined with MS are briefly summarized.
Generic Security Service Application Program Interface, Version 2 (1997)

The Generic Security Service Application Program Interface (GSS-API), as defined in RFC-1508, provides security services to callers in a generic fashion, supportable with a range of underlying mechanisms and technologies and hence allowing source-level portability of applications to different environments. This specification defines GSS-API services and primitives at a level independent of underlying mechanism and programming language environment, and is to be complemented by other, related specifications:
Sacred groves in conservation of plant biodiversity in Banaskantha district, Gujarat, India. (2013)

Nature conservation practices are very ancient tradition in India. Useful biodiversity species have much reverence in culture of our country. Sacred groves are seen through out Gujarat, having varied forms, cultural practices and belief systems. The vegetation in the groves is highly varied viz. mangroves, fresh water swamps, or other tropical forest types. Sacred  groves represent long   tradition of environmental conservation based on indigenous knowledge by the tribal communities of Banaskantha District, Gujarat, India. The study deals with the role of sacred groves in conservation of plant biodiversity in Banaskantha District, Gujarat, India. Among the 11 sacred groves surveyed, over 25 different plant species are reported. In the sacred groves surveyed, only a few are well protected while most are partially threatened due to anthropogenic pressure.  Considering all the dimensions of sacred groves, it is clear that these groves are considered as one of the most species-rich areas for plants, birds and mammals.
Peak power reduction scheme based on subcarrier scrambling for MC-CDMA systems (2004)

A new PAPR (peak-to-average power ratio) reduction scheme is proposed for MC-CDMA (multicarrier-CDMA) using subcarrier scrambling. Based on the fact that the PAPR of the MC-CDMA signal is highly dependent on the scrambling pattern of the subcarriers irrespective of users&apos; data symbols, the optimum fixed scrambling patterns for each combination of user spreading codes are searched to achieve the minimum PAPR. This enables the system to dynamically allocate the optimum scrambling pattern during system operation. The proposed scheme achieves a significant reduction in PAPR compared to random scrambling, especially for a small number of users. A simple suboptimum scrambling scheme is also proposed where a common scrambling pattern is used irrespective of the code combinations.
Redox Cycling in Nanopore-Confined Recessed Dual-Ring Electrode Arrays (2016)

A redox cycling geometry based on an array of nanopore-confined recessed dual-ring electrodes (RDREs) has been devised to amplify electrochemical signals and enhance the sensitivity of electroanalytical measurements. The RDRE arrays were fabricated using layer-by-layer deposition followed by focused ion beam milling. A characteristic feature of the nanoscale dual-ring geometry is that electrochemical reactions occurring at the bottom-ring electrode can be tuned by modulating the potential at the top-ring electrode. Thus, the resulting device was operated in generator–collector mode by holding the top-ring electrodes at a constant potential and performing cyclic voltammetry by sweeping the bottom-ring potential in aqueous Fe(CN)63–/4–. The enhanced (∼23×) limiting current, achieved by cycling the redox couple between top- and bottom-ring electrodes with high collection efficiency, was compared with that obtained in the absence of self-induced redox cycling (SIRC). Measured shifts in Fe(CN)63–/4– concentration distributions were found to be in excellent agreement with finite-element simulations. The SIRC effect in the RDRE array was also characterized by electrochemical experiments before and after oxygen plasma treatment. The plasma-treated RDRE array exhibited a significant signal amplification, with the faradaic current being augmented by a factor of ∼65 as a result of efficient redox cycling of electroactive species in the nanopores. The amplification factor of the devices was optimized by controlling the interpore distance, with larger pore density arrays exhibiting larger amplification factors.
Macromarketing: A Social Systems Perspective (1984)

The marketing system is a differentiated subsystem of the society, performing functions necessary if society is to adapt to its material environment. In the performance of these functions, the marketing system has an impact on other social systems, the cultural system, and the material environment. This dual relationship is derived from a modified social systems paradigm.
Homero eta gu: itzulpen bat(zu)en inguruko oharrak eta beste (2020)

Artikulu honek abiaburu gisa hartzen du Emily Wilsonen 'Odisea'ren itzulpena, zeinak auzitan jartzen baititu ingeleserako itzulpen kanonikoek errepikaturiko zenbait konbentzio sexista, eta genero-ikuspuntu berezi bat ematen baitio bere itzulpen-poetikari. Konbentzio horietako batzuei erreparatzen die artikuluak, eta, jarraian, azterketa informal bat egiten du gaur egun antzinako Greziako eta Erromako literatura-lanek euskal literaturan duten presentziari buruz. Azaltzen du nola lehen euskal itzultzaile sistematikoek, 1950eko urteen ingurukoek, ezinago garrantzitsutzat jotzen zuten kanon klasiko hori euskaratzea. Euskaratu beharreko kanon hori, baina, nabarmen aldatuz joan da, 1990eko hamarkadan barrena gaur egun arte, non egile greko-latinoak baztertuta geratu baitira. Azken gogoeta gisa, artikuluak defendatzen du bateratu egin behar direla historikoki bazterturiko zenbait subjekturen (emakume, Mendebaldeaz besteko) presentzia handitzeko eginahalak eta literatura greko-latinoaren berreskuratzea, Emily Wilsonen lana lekuko.
Purification and membrane reconstitution of catalytically active Menkes copper-transporting P-type ATPase (MNK; ATP7A) (2006)

The MNK (Menkes disease protein; ATP7A) is a major copper- transporting P-type ATPase involved in the delivery of copper to cuproenzymes in the secretory pathway and the efflux of excess copper from extrahepatic tissues. Mutations in the MNK (ATP7A) gene result in Menkes disease, a fatal neurodegenerative copper deficiency disorder. Currently, detailed biochemical and biophysical analyses of MNK to better understand its mechanisms of copper transport are not possible due to the lack of purified MNK in an active form. To address this issue, we expressed human MNK with an N-terminal Glu-Glu tag in Sf9 [Spodoptera frugiperda (fall armyworm) 9] insect cells and purified it by antibody affinity chromatography followed by size-exclusion chromatography in the presence of the non-ionic detergent DDM (n-dodecyl beta-D-maltopyranoside). Formation of the classical vanadate-sensitive phosphoenzyme by purified MNK was activated by Cu(I) [EC50=0.7 microM; h (Hill coefficient) was 4.6]. Furthermore, we report the first measurement of Cu(I)-dependent ATPase activity of MNK (K0.5=0.6 microM; h=5.0). The purified MNK demonstrated active ATP-dependent vectorial 64Cu transport when reconstituted into soya-bean asolectin liposomes. Together, these data demonstrated that Cu(I) interacts with MNK in a co-operative manner and with high affinity in the sub-micromolar range. The present study provides the first biochemical characterization of a purified full-length mammalian copper-transporting P-type ATPase associated with a human disease.
Enhanced Pictorial Structures for precise eye localization under incontrolled conditions (2009)

In this paper, we present an enhanced pictorial structure (PS) model for precise eye localization, a fundamental problem involved in many face processing tasks. PS is a computationally efficient framework for part-based object modelling. For face images taken under uncontrolled conditions, however, the traditional PS model is not flexible enough for handling the complicated appearance and structural variations. To extend PS, we 1) propose a discriminative PS model for a more accurate part localization when appearance changes seriously, 2) introduce a series of global constraints to improve the robustness against scale, rotation and translation, and 3) adopt a heuristic prediction method to address the difficulty of eye localization with partial occlusion. Experimental results on the challenging LFW (Labeled Face in the Wild) database show that our model can locate eyes accurately and efficiently under a broad range of uncontrolled variations involving poses, expressions, lightings, camera qualities, occlusions, etc.
Pathophysiological Basis and Rationale for Early Outpatient Treatment of SARS-CoV-2 (COVID-19) Infection (2020)

Approximately 9 months of the severe acute respiratory syndrome coronavius-2 (SARS-CoV-2 [COVID-19]) spreading across the globe has led to widespread COVID-19 acute hospitalizations and death. The rapidity and highly communicable nature of the SARS-CoV-2 outbreak has hampered the design and execution of definitive randomized, controlled trials of therapy outside of the clinic or hospital. In the absence of clinical trial results, physicians must use what has been learned about the pathophysiology of SARS-CoV-2 infection in determining early outpatient treatment of the illness with the aim of preventing hospitalization or death. This article outlines key pathophysiological principles that relate to the patient with early infection treated at home. Therapeutic approaches based on these principles include 1) reduction of reinoculation, 2) combination antiviral therapy, 3) immunomodulation, 4) antiplatelet/antithrombotic therapy, and 5) administration of oxygen, monitoring, and telemedicine. Future randomized trials testing the principles and agents discussed will undoubtedly refine and clarify their individual roles; however, we emphasize the immediate need for management guidance in the setting of widespread hospital resource consumption, morbidity, and mortality.
Structured Attention Networks (2017)

Attention networks have proven to be an effective approach for embedding categorical inference within a deep neural network. However, for many tasks we may want to model richer structural dependencies without abandoning end-to-end training. In this work, we experiment with incorporating richer structural distributions, encoded using graphical models, within deep networks. We show that these structured attention networks are simple extensions of the basic attention procedure, and that they allow for extending attention beyond the standard soft-selection approach, such as attending to partial segmentations or to subtrees. We experiment with two different classes of structured attention networks: a linear-chain conditional random field and a graph-based parsing model, and describe how these models can be practically implemented as neural network layers. Experiments show that this approach is effective for incorporating structural biases, and structured attention networks outperform baseline attention models on a variety of synthetic and real tasks: tree transduction, neural machine translation, question answering, and natural language inference. We further find that models trained in this way learn interesting unsupervised hidden representations that generalize simple attention.
Accessing to the minor proteome of red blood cells through the influence of the nanoparticle surface properties on the corona composition (2015)

Nanoparticle (NP)-protein interactions in complex samples have not yet been clearly understood. Nevertheless, several studies demonstrated that NP's physicochemical features significantly impact on the protein corona composition. Taking advantage of the NP potential to harvest different subsets of proteins, we assessed for the first time the capacity of three kinds of superparamagnetic NPs to highlight the erythrocyte minor proteome. Using both qualitative and quantitative proteomics approaches, nano-liquid chromatography-tandem mass spectrometry allowed the identification of 893 different proteins, confirming the reproducible capacity of NPs to increase the number of identified proteins, through a reduction of the sample concentration range and the capture of specific proteins on the three different surfaces. These NP-specific protein signatures revealed significant differences in their isoelectric point and molecular weight. Moreover, this NP strategy offered a deeper access to the erythrocyte proteome highlighting several signaling pathways implicated in important erythrocyte functions. The automated potentiality, the reproducibility, and the low-consuming sample demonstrate the strong compatibility of our strategy for large-scale clinical studies and may become a standardized sample preparation in future erythrocyte-associated proteomics studies.
The Progressive Smart Grid System from Both Power and Communications Aspects (2011)

The present electric power system structure has lasted for decades; it is still partially proprietary, energy-inefficient, physically and virtually (or cyber) insecure, as well as prone to power transmission congestion and consequent failures. Recent efforts in building a smart grid system have focused on addressing the problems of global warming effects, rising energy-hungry demands, and risks of peak loads. One of the major goals of the new system is to effectively regulate energy usage by utilizing the backbone of the prospectively deployed Automatic Meter Reading (AMR), Advanced Meter Infrastructure (AMI), and Demand Response (DR) programs via the advanced distribution automation and dynamic pricing models. The function of the power grid is no longer a system that only supplies energy to end users, but also allows consumers to contribute their clean energy back to the grid in the future. In the meantime, communications networks in the electric power infrastructure enact critical roles. Intelligent automation proposed in smart grid projects include the Supervisory Control And Data Acquisition/Energy Management Systems (SCADA/EMS) and Phasor Management Units (PMU) in transmission networks, as well as the AMR/AMI associated with field/neighborhood area networks (FAN/NAN) and home area networks (HAN) at the distribution and end-use levels. This article provides an overview of the essentials of the progressive smart grid paradigm and integration of different communications technologies for the legacy power system. Additionally, foreseeable issues and challenges in designing communications networks for the smart grid system are also rigorously deliberated in this paper.
Fold Rise in Antibody Titers by Measured by Glycoprotein-Based Enzyme-Linked Immunosorbent Assay Is an Excellent Correlate of Protection for a Herpes Zoster Vaccine, Demonstrated via the Vaccine Efficacy Curve (2014)

NCT00534248.
Mechanisms and Models in Heart Failure (1999)

The people who bind themselves to systems are those who are unable to encompass the whole truth and try to catch
Ciprofloxacin-resistantSalmonellaKentucky in Travelers (2006)

To the Editor: Ciprofloxacin is the treatment of choice of severe nontyphoidal Salmonella infections in adults. Resistance to ciprofloxacin has been found exceptionally in nontyphoidal Salmonella enterica isolates and only in serotypes Typhimurium, Choleraesuis, and Schwarzengrund (1-8). Such isolates have been collected from humans and animals in Europe, Asia, and North America.
In vitro activity of thalidomide against Mycobacterium avium complex (1993)

In a previous article,<sup>1</sup>we described two patients with acquired immunodeficiency syndrome with<i>Mycobacterium avium</i>complex (MAC) bacteremia, prolonged fever, and weight loss, refractory to standard therapy, who responded to the administration of thalidomide. The activity of thalidomide was probably related to its immunomodulatory effect; it is not unusual to exclude activity of the drug against MAC. To ascertain the potential effectiveness of thalidomide against MAC, we tested its "in vitro" activity against 36 different MAC strains by the agar dilution method.<sup>2,3</sup>A 10<sup>4</sup>-μg/mL stock solution was prepared using dimethylformamide as a solvent. Afterward, serial twofold dilutions were made in sterile distilled water and incorporated into an agar with a pH of 6.8 (7H10 Middlebrook) to obtain the following final concentrations: 512, 256, 128, 64, 32, 16, 8, 4, 2, 1, and 0.5 μg/L, respectively. Inocula were prepared from every strain growth on Löwenstein-Jensen media in
Automatic playlist composition in a dynamic music landscape (2007)

Music is both a social and a personal experience. Our goal is to automatically compose music playlists that combine the personal expectations of the listener with the expertise of professional playlist composers (or DJs).
Cisplatin in advanced cancer of the cervix: an update. (1991)

Cisplatin is the single most active cytotoxic agent in the treatment of patients with recurrent or metastatic squamous cell cancer of the cervix; no other standard drug has been associated consistently with objective response rates of 25% or higher. However, cervical cancer is a relatively drug-resistant disease and prolonged cisplatin treatment appears to induce multiple mechanisms of tumor resistance. The majority of objective responses to cisplatin are partial and relatively short-lived and, therefore, have little impact on survival duration. Complete responses to cisplatin are seen predominantly in patients with extrapelvic metastases rather than pelvic recurrences. This article examines the role of cisplatin in the management of advanced cervical cancer with emphasis on its efficacy in the following circumstances: as a single agent; in combination with other cytotoxic drugs; in previously treated patients; and when administered before surgery or before or concurrent with definitive radiation therapy.
Targeting LAG-3 and PD-1 to Enhance T Cell Activation by Antigen-Presenting Cells (2018)

Immune checkpoint inhibition has been shown to successfully reactivate endogenous T cell responses directed against tumor-associated antigens, resulting in significantly prolonged overall survival in patients with various tumor entities. For malignancies with low endogenous immune responses, this approach has not shown a clear clinical benefit so far. Therapeutic vaccination, particularly dendritic cell (DC) vaccination, is a strategy to induce T cell responses. Interaction of DCs and T cells is dependent on receptor-ligand interactions of various immune checkpoints. In this study, we analyzed the influence of blocking antibodies targeting programmed cell death protein 1 (PD-1), HVEM, CD244, TIM-3, and lymphocyte activation gene 3 (LAG-3) on the proliferation and cytokine secretion of T cells after stimulation with autologous TLR-matured DCs. In this context, we found that LAG-3 blockade resulted in superior T cell activation compared to inhibition of other pathways, including PD-1/PD-L1. This result was consistent across different methods to measure T cell stimulation (proliferation, IFN-γ secretion), various stimulatory antigens (viral and bacterial peptide pool, specific viral antigen, specific tumor antigen), and seen for both CD4<sup>+</sup> and CD8<sup>+</sup> T cells. Only under conditions with a weak antigenic stimulus, particularly when combining antigen presentation by peripheral blood mononuclear cells with low concentrations of peptides, we observed the highest T cell stimulation with dual blockade of LAG-3 and PD-1 blockade. We conclude that priming of novel immune responses can be strongly enhanced by blockade of LAG-3 or dual blockade of LAG-3 and PD-1, depending on the strength of the antigenic stimulus.
Efficacy of topical 1% lidocaine in the symptomatic treatment of pain associated with oral mucosal trauma or minor oral aphthous ulcer: a randomized, double-blind, placebo-controlled, parallel-group, single-dose study. (2011)

A significant reduction in pain intensity occurred after application of 1% lidocaine cream and was significantly greater than that with the placebo cream. Taking into account the study's limitations, this product seems safe to use.
[Prolonged diarrhea in infancy: effect of deconjugated bile acids and cholestyramine action]. (1980)

527 patients whose ages ranged from 3 months to 5 years, with acute diarrhea were studied. 517 showed satisfactory response to treatment. Diarrhea persisted in 10 patients (2 %) and bile acids were determined in intestinal juice before and after cholestyramine administration. 7 patients showed bile acids deconjugation, 6 of which cured clinically. 3 patients showed no deconjugation, and 1 of them improved after cholestyramine therapy. Side effects of drug were not observed.