gebhart/patrepeval huggingface dataset:
The Kinetics of the Synthesis of Photopigments in Rhodopseudomonas spheroides (1962)

SUMMARY: The magnitude of self-shading in light-grown cultures of Rhodopseudomonas spheroides has been estimated. When a culture of R. spheroides is transferred from bright light to dim light there is preferential synthesis of bacteriochlorophyll and carotenoid pigments relative to net protein synthesis. Protein turnover does not account for this. The kinetics of the synthesis of bacteriochlorophyll relative to the incorporation of amino acids into protein have been estimated under a variety of conditions. On the basis of these findings a mechanism for the cellular control of pigment synthesis is presented.
Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination (2007)

We report the isolation, purification, genome-sequencing and characterization of a picorna-like virus from dead bees in Israel. Sequence analysis indicated that IAPV (Israeli acute paralysis virus) is a distinct dicistrovirus. It is most homologous to Kashmir bee virus and acute bee paralysis virus. The virus carries a 9487 nt RNA genome in positive orientation, with two open reading frames separated by an intergenic region, and its coat comprises four major proteins, the sizes of which suggest alternate processing of the polyprotein. IAPV virions also carry shorter, defective-interfering (DI)-like RNAs. Some of these RNAs are recombinants of different segments of IAPV RNA, some are recombinants of IAPV RNA and RNA from another dicistrovirus, and yet others are recombinants of IAPV and non-viral RNAs. In several of the DI-like RNAs, a sense-oriented fragment has recombined with its complement, forming hairpins and stem-loop structures. In previous reports, we have shown that potyviral and IAPV sequences are integrated into the genome of their respective hosts. The dynamics of information exchange between virus and host and the possible resistance-engendering mechanisms are discussed.
Chemical Typing of Amyloid Protein Contained in Formalin-Fixed Paraffin-Embedded Biopsy Specimens (2001)

The human amyloidoses represent a heterogeneous group of disorders characterized by the deposition of fibrillar protein in vital organs. Given the fact that at least 20 different molecules can form fibrils, the unambiguous identification of the type of amyloid deposited is critical to the correct diagnosis and treatment of patients with these disorders. Heretofore, this information has been inferred from particular clinical features of the disease, ancillary laboratory tests, and results of immunohistochemical analyses. However, to establish unequivocally the kind of protein that is deposited as amyloid, it is necessary to determine its chemical composition through amino acid sequencing or mass spectroscopy of material extracted from fibrillar deposits. We have developed a micromethod whereby such studies can be performed readily using sections of formalin-fixed, paraffin-embedded biopsy specimens. The ability to identify precisely the nature of the tissue deposits has diagnostic, therapeutic, and prognostic implications for patients with amyloid-associated disorders.
Use of phosphorus oxychloride in synthesizing nucleotides and ollgonucleotides (1974)

Procedures are described for phosphorylating protected nucleotides, oligonucleotides and phosphoramidate oligonucleotide derivatives at the 3′-hydroxyl group. The conditions (phosphorylation with phosphorus oxychloride and pyridine in dioxane followed by hydrolysis with aqueous pyridine) are sufficiently mild that base labile (trifluoroacetylamino; β-cyanoethyl phosphotriester) and acid labile (O-monomethoxytrityl; phosphoramidate) functions are retained intact. Application of the technique is illustrated by the synthesis of dpT, dTp, d(CF3CONH)Tp, dTpNTp, and dTpNTpNTp. In addition, the utilization of phosphorus oxychloride in joining thymidine derivatives and dinucleoside phosphotriester blocks via phosphodiester links is described.
Two-dimensional materials for miniaturized energy storage devices: from individual devices to smart integrated systems (2018)

Nowadays, the increasing requirements of portable, implantable, and wearable electronics have greatly stimulated the development of miniaturized energy storage devices (MESDs). Electrochemically active materials and microfabrication techniques are two indispensable parts in MESDs. Particularly, the architecture design of microelectrode arrays is beneficial to the accessibility of two-dimensional (2D) active materials. Therefore, this study reviews the recent advancements in microbatteries and microsupercapacitors based on electrochemically active 2D materials. Emerging microfabrication strategies enable the precise control over the thickness, homogeneity, structure, and dimension in miniaturized devices, which offer tremendous opportunities for achieving both high energy and power densities. Furthermore, smart functions and integrated systems are discussed in detail in light of the emergence of intelligent and interactive modes. Finally, future developments, opportunities, and urgent challenges related to 2D materials, device fabrications, smart responsive designs, and microdevice integrations are provided.
A Continuous Flow Synthesis of Micrometer‐Sized Actuators from Liquid Crystalline Elastomers (2009)

We demonstrate the use of a microfluidic setup to prepare monodisperse and spherical beads from a liquid crystalline elastomer. These particles show a strong and reversible shape change into a cigar-like conformation during the transition into the isotropic phase. These properies are a result of the monodomainic alignment of the mesogens in the flow field of the microfluidic setup. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
H3 Relaxin Is a Specific Ligand for LGR7 and Activates the Receptor by Interacting with Both the Ectodomain and the Exoloop 2 (2003)

Leucine-rich repeat-containing, G protein-coupled receptors (LGRs) represent a unique subgroup of G protein-coupled receptors with a large ectodomain. Recent studies demonstrated that relaxin activates two orphan LGRs, LGR7 and LGR8, whereas INSL3/Leydig insulin-like peptide specifically activates LGR8. Human relaxin 3 (H3 relaxin) was recently discovered as a novel ligand for relaxin receptors. Here, we demonstrate that H3 relaxin activates LGR7 but not LGR8. Taking advantage of the overlapping specificity of these three ligands for the two related LGRs, chimeric receptors were generated to elucidate the mechanism of ligand activation of LGR7. Chimeric receptor LGR7/8 with the ectodomain from LGR7 but the transmembrane region from LGR8 maintains responsiveness to relaxin but was less responsive to H3 relaxin based on ligand stimulation of cAMP production. The decreased ligand signaling was accompanied by decreases in the ability of H3 relaxin to compete for (33)P-relaxin binding to the chimeric receptor. However, replacement of the exoloop 2, but not exoloop 1 or 3, of LGR7 to the chimeric LGR7/8 restored ligand binding and receptor-mediated cAMP production. These results suggested that activation of LGR7 by H3 relaxin involves specific binding of the ligand to both the ectodomain and the exoloop 2, thus providing a model with which to understand the molecular basis of ligand signaling for this unique subgroup of G protein-coupled receptors.
Randomized controlled trial of cannabis‐based medicine in spasticity caused by multiple sclerosis (2007)

Symptoms relating to spasticity are common in multiple sclerosis (MS) and can be difficult to treat. We have investigated the efficacy, safety and tolerability of a standardized oromucosal whole plant cannabis-based medicine (CBM) containing delta-9 tetrahydrocannabinol (THC) and cannabidiol (CBD), upon spasticity in MS. A total of 189 subjects with definite MS and spasticity were randomized to receive daily doses of active preparation (n = 124) or placebo (n = 65) in a double blind study over 6 weeks. The primary endpoint was the change in a daily subject-recorded Numerical Rating Scale of spasticity. Secondary endpoints included a measure of spasticity (Ashworth Score) and a subjective measure of spasm. The primary efficacy analysis on the intention to treat (ITT) population (n = 184) showed the active preparation to be significantly superior (P = 0.048). Secondary efficacy measures were all in favour of active preparation but did not achieve statistical significance. The responder analysis favoured active preparation, 40% of subjects achieved >30% benefit (P = 0.014). Eight withdrawals were attributed to adverse events (AEs); six were on active preparation and two on placebo. We conclude that this CBM may represent a useful new agent for treatment of the symptomatic relief of spasticity in MS.
Duration of antimicrobial therapy for non-typhoid Salmonella bacteremia in healthy children. (2002)

Medical records of patients less than 17 years of age with positive blood culture for non-typhoid Salmonella from June 1996 through December 1998 at the Chang Gung Children's Hospital were collected. Patients were categorized into 3 groups according to the duration of effective antibiotic therapy they received. Group 1 received effective antibiotic therapy for less than 7 days; Group 2 for 7 to 9 days; and Group 3 for 10 days or more. Information on clinical presentations, laboratory data, and outcome were collected and analyzed. A total of 59 children were eligible, with 21 in Group 1, 17 in Group 2, and 21 in Group 3. Demographic data were similar among the 3 groups. No significant difference was found in the clinical symptoms and laboratory data among these groups, including white blood cell count, immature to total ratio of white blood cell, and serum C-reactive protein level. Salmonella group B (n = 37) were the most common isolates, followed by group D (n = 9), group C2 (n = 8), cholerasuis (n = 4), and C1 (n = 2). Third-generation cephalosporins were the final antibiotic therapy in most (90%) patients, and no isolates showed resistance to these agents in this study. No focal suppurative complications were noted at the initial evaluation and in the 1-year period after treatment. No clinical recurrent diseases were noted during a follow-up period of at least 1 year. Results suggest that for otherwise healthy children with non-typhoid Salmonella bacteremia, less than 10 days of antibiotics therapy could be adequate if they are more than 1 year old and no focal extra-intestinal infection is noted in the initial evaluation.
Diagnosing abdominal pain in a pediatric emergency department (1992)

We undertook a prospective study of 377 children (two to 16 years old) presenting with abdominal pain to determine: 1) common discharge diagnoses; 2) what signs and symptoms are associated with appendicitis; and 3) follow-up of patients discharged from the emergency department (ED). Nine diagnoses accounted for 86% of all diagnoses made. The most common final diagnosis was "abdominal pain" (36%). The following findings were significantly associated with appendicitis: vomiting, right lower quadrant(RLQ) pain, tenderness, and guarding (all P less than 0.001). Ninety-seven percent (28/29) of patients with appendicitis had at least two of these four signs and symptoms, as did 28% (96/348) of patients without appendicitis. The sensitivity of the model is 0.96, and the specificity is 0.72 (positive predictive value = 0.24; negative predictive value = 0.99). Of the patients contacted within one week of the visit (237), 75% reported that the pain had resolved (mean contact time, 2.6 days). We conclude that 1) patients presenting to the ED with abdominal pain often leave with the diagnosis of abdominal pain; 2) of the patients contacted, the majority reported that their pain has resolved; and 3) a diagnosis of appendicitis should be considered in any patient with any two of the following signs or symptoms: vomiting, guarding, tenderness, or RLQ pain. Such patients should be evaluated and observed carefully for the possible diagnosis of appendicitis.
A Heat-And-Moisture Exchanger for Post-Tracheotomy CareAn Experimental Study (1960)

AbstractThis paper deals with the variations in perspiratio insensibilis during normal nasal respiration and breathing through a tracheostoma. Widely used methods for moistening the inspired air of tracheotomized patients are listed and discussed. An aluminium foil heat-and-moisture exchanger (HME) for direct application to the tracheal cannula is described and tested experimentally in order to determine its air flow resistance as well as its heat and moisture recovery efficiency.
Future prospects of MRAM technologies (2013)

This paper presents a review and future prospects for the tunnel magnetoresistance (TMR) effect in magnetic tunnel junction (MTJ) and spin manipulation technologies such as spin-transfer torque (STT) for magnetoresistive random access memory (MRAM). Major challenges for ultrahigh-density STT-MRAM with perpendicular magnetization and novel functional devices related to MRAM are discussed.
A Study of Early Stage Self-Loosening of Bolted Joints (2003)

Both experimental investigation and finite element analysis were conducted to explore the mechanisms for the early stage self-loosening of bolted joints under transverse cyclic loading. The nuts were glued to the bolts using a strong thread locker in the self-loosening experiments to ensure that no backing-off of the nut occurred. Depending on the loading magnitude, the clamping force reduction ranged from 10% to more than 40% of the initial preload after 200 loading cycles. Three-dimensional elastic-plastic finite element analysis was conducted with the implementation of an advanced cyclic plasticity model. The finite element results revealed that the local cyclic plasticity occurring near the roots of the engaged threads resulted in cyclic strain ratcheting. The localized cyclic plastic deformation caused the stresses to redistribute in the bolt, and the result was the gradual loss of clamping force with loading cycles. The finite element results agreed with the experimental observations quantitatively. When the two clamped plates started to slip and the slip displacement was controlled, both experiments and finite element simulations suggested that the friction between the clamped plates has an insignificant influence on the early stage self-loosening.
Localization of sites within dorsal motor nucleus of vagus that affect gastric motility (1985)

The purpose of our study was to determine the localization of sites within the dorsal motor nucleus of the vagus (DMV) of the cat that when stimulated would increase gastric motility. To do this, two types of experiments were performed. First, the retrograde tracer fast blue was injected into the antrum and pylorus, and labeled neurons in the DMV were identified. Second, electrical stimulation was performed in areas of the DMV labeled with fast blue as well as in nearby areas with no labeling while monitoring gastric motility, arterial pressure, and heart rate. Results from the first type of studies revealed that peak labeling in the DMV occurred between 0.56 and 1.56 mm rostral to obex. Electrical stimulation in this area using 100 microA, 0.2 ms duration pulses, and 50 Hz resulted in increases in antral and pyloric contractions in 20 animals. The magnitude of pyloric and antral responses elicited by stimulation of the DMV generally correlated to the number of cell bodies labeled with fast blue within the DMV. No changes in arterial pressure occurred, and only a slight (-4%) decrease in heart rate was observed. Maximal increases in motility occurred with 20 Hz (antrum) or 100 Hz (pylorus). These increases in motility were maintained even at 200- and 400-Hz stimulation. Ipsilateral vagotomy or pretreatment with propantheline bromide prevented the increases in gastric motility produced by electrical stimulation of the DMV. Electrical stimulation of more rostral sites in the DMV, the medial nucleus of the solitary tract (NTS), and an area within 1.0 mm medial to the DMV resulted in attenuated or no motility responses. Stimulation of the medial nucleus of the NTS did result in pronounced slowing in heart rate (-61 +/- 21 beats/min). These results suggest that there is a localization of a "stomach area" within the DMV and that electrical stimulation of this area results in gastric motility responses that are mediated by vagal fibers projecting directly to the stomach. In addition, electrical stimulation of the DMV results in selective effects on the gastrointestinal tract in that no pronounced changes in heart rate and arterial pressure occur.
Biotechnological Production of Lactic Acid and Its Recent Applications (2006)

Lactic acid is widely used in the food, cosmetic, pharmaceutical, and chemical industries and has received increased attention for use as a monomer for the production of biodegradable poly(lactic acid). It can be produced by either biotechnological fermentation or chemical synthesis, but the former route has received considerable interest recently, due to environmental concerns and the limited nature of petrochemical feedstocks. There have been various attempts to produce lactic acid efficiently from inexpensive raw materials. We present a review of lactic acid-producing microorganisms, raw materials for lactic acid production, fermentation approaches for lactic acid production, and various applications of lactic acid, with a particular focus on recent investigations. In addition, the future potentials and economic impacts of lactic acid are discussed.
NF-κB/Rel Participation in the Lymphokine-Dependent Proliferation of T Lymphoid Cells (2001)

Proliferative responses of lymphoid cells to IL-2 and IL-4 depend on activation of the cells, but the mechanism(s) by which activation enhances cellular competence to respond to cytokines is not fully understood. The NF-kappaB/Rel family represents one signal transduction pathway induced during such activation. We show in this study that inhibition of NF-kappaB through the expression of an IkappaBalpha (inhibitory protein that dissociates from NF-kappaB) mutant refractory to signal-induced degradation (IkappaBalpha(DeltaN)) interfered with the acquisition of competence to proliferate in response to IL-4 as well as IL-2. Thymocytes and T cells from IkappaBalpha(DeltaN) transgenic mice expressed normal levels of IL-2R subunits. However, transgenic cells exhibited a dramatic defect in Stat5A activation treatment with IL-2, and a similar defect was observed for IL-4-induced Stat5. In contrast, T lymphoid cells with inhibition of NF-kappaB showed normal insulin receptor substrate-2 phosphorylation and only a modest decrease in Stat6 activation and insulin receptor substrate-1 phosphorylation after IL-4 stimulation. These results indicate that the NF-kappaB/Rel/IkappaBalpha system can regulate cytokine receptor capacitation through effects on the induction of downstream signaling by the Stat transcription factor family.
Interdiffusion and self-diffusion in polymer mixtures: A Monte Carlo study (1991)

A lattice model for dense polymer solutions and polymer mixtures in three dimensions is presented, aiming to develop a model suitable for efficient computer simulation on vector processors, with a qualitatively realistic local dynamics. It is shown that the bond fluctuation algorithm for a suitable set of allowed bond vectors has the property that due to the excluded volume constraint no crossing of bonds by local motions can occur, and entanglement restrictions thus are fully taken into account. For athermal binary (AB) symmetrical polymer mixtures, the dependence of both self-diffusion coefficient and interdiffusion coefficient on polymer density is obtained, simulating a thin film geometry where a film of polymer A is coated with a film of polymer B. For one density, the dependence of the interdiffusion coefficient on an attractive energy between unlike monomers is also studied. For weak attraction an enhancement of interdiffusion proportional to this energy occurs. For strong attraction, however, a rather immobile tightly bound AB layer forms in the interface which hampers further unmixing.
Ocular Cell Monolayers Cultured on Biodegradable Substrates (1999)

The aim of this study was to culture retinal pigment epithelial (RPE) and corneal endothelial cells on biodegradable substrates for future use in monolayer transplantation in the eye. The biodegradable polymers, poly-l-lactic (PLLA) and poly-dl-lactic-co-glycolic acid (85:15) (PLGA) (both of molecular weight 105 kd) were the biomaterials used. All materials were seeded with either pig/human retinal pigment epithelial cells or rabbit corneal endothelial cells and were maintained in tissue culture conditions. Upon confluency, the cell density was calculated and cell viability determined. All monolayers were stained with phalloidin-rhodamine for F-actin and antibodies to the tight junction (zonula occludens) protein, ZO1, to demonstrate the presence of tight junctions. The final cell density of human RPE monolayers on PLLA films was 2950 cells/mm(2) (+/-185). The final cell density of pig RPE on PLLA and PLGA film was 2350 cells/mm(2) (+/-152 and 178, respectively). Rabbit corneal endothelial cells had a final cell density of 2650 cells/mm(2) (+/-164). F-actin staining revealed a circumferential ring of actin filaments in all of the cells grown on substrates. ZO(1) immunohistochemistry demonstrated staining along the lateral cell borders of all cell types. The successful culture of retinal pigment epithelial and corneal endothelial monolayers on these substrates may have potential for transplanting cell monolayers in the eye to improve vision.
Receptor subtype-specific pronociceptive and analgesic actions of galanin in the spinal cord: Selective actions via GalR1 and GalR2 receptors (2001)

Galanin is a 29-aa neuropeptide with a complex role in pain processing. Several galanin receptor subtypes are present in dorsal root ganglia and spinal cord with a differential distribution. Here, we describe a generation of a specific galanin R2 (GalR2) agonist, AR-M1896, and its application in studies of a rat neuropathic pain model (Bennett). The results show that in normal rats mechanical and cold allodynia of the hindpaw are induced after intrathecal infusion of low-dose galanin (25 ng per 0.5 microl/h). The same effect is seen with equimolar doses of AR-M1896 or AR-M961, an agonist both at GalR1 and GalR2 receptors. In allodynic Bennett model rats, the mechanical threshold increased dose-dependently after intrathecal injection of a high dose of AR-M961, whereas no effect was observed in the control or AR-M1896 group. No effect of either of the two compounds was observed in nonallodynic Bennett model rats. These data indicate that a low dose of galanin has a nociceptive role at the spinal cord level mediated by GalR2 receptors, whereas the antiallodynic effect of high-dose galanin on neuropathic pain is mediated by the GalR1 receptors. Thus, a selective GalR1 agonist may be used to treat neuropathic pain.