gebhart/patrepeval huggingface dataset:
Covalent structure of two novel neutrophile leucocyte‐derived proteins of porcine and human origin (1991)

The covalent structures of two, novel, neutrophile, leucocyte-derived, strongly basic proteins of porcine and human origin have been determined by microsequencing in combination with time-of-flight plasma desorption mass spectrometry. The porcine protein primary structure of 219 amino acid residues was shown to contain 6 cysteine residues, 2 putative carbohydrate sites and 14% basic residues. The human protein contained 221 amino acid residues of which 8 were cysteine, 4 putative carbohydrate sites and 12% basic. A 47% direct sequence similarity to human neutrophile elastase was found, but due to mutations of two of the three amino acids in the catalytic triad, proteolytic activity is absent. Modelling and alignment studies unveil a close relationship of both proteins to the serine protease family, the greatest similarity being to those serine proteases present in granules from peripheral blood cells. Both proteins have been shown to be chemotactically active for monocytes and fibroblasts in vitro.
Characterization of the full length cDNA for murine beta-1,4-galactosyltransferase. Novel features at the 5′-end predict two translational start sites at two in-frame AUGs. (1988)

We have isolated overlapping cDNA clones representing the full length (4038 base pairs) transcript for murine beta-1,4-galactosyltransferase. The coding sequence predicts a membrane-bound glycoprotein with three distinct structural features, a large COOH-terminal domain (355 amino acids), a single transmembrane domain (20 amino acids), and a short NH2-terminal domain. Primer extension analysis, S1 protection analysis, and RNA blotting demonstrate the presence of two sets of mRNA transcripts which differ in length by about 200 base pairs. The 5' boundary of the long transcripts maps upstream of two in-frame ATGs. The 5' boundary of the short transcripts maps between these two ATGs. These results predict that two related forms of beta-1,4-galactosyltransferase of 399 and 386 amino acids are synthesized as a consequence of alternative translation initiation. The long form (399 amino acids) has an NH2-terminal extension of 13 amino acids.
Analysis of failure sources in surface-micromachined MEMS (2002)

The effect of vertical stiction, foreign particles, and etch variation on the resonant frequency of a surface-micromachined resonator and accelerometer are presented. For each device, it is shown that misbehaviors resulting from different failure sources can overlap, exhibit dominance and combine to create behavior masking and construction. Such an analysis is essential for developing test and diagnosis methodologies for surface-micromachined MEMS.
Characterization of a New Class of Potent Inhibitors of the Voltage-Gated Sodium Channel Nav1.7 (2007)

Voltage-gated sodium channels (Nav1) transmit pain signals from peripheral nociceptive neurons, and blockers of these channels have been shown to ameliorate a number of pain conditions. Because these drugs can have adverse effects that limit their efficacy, more potent and selective Nav1 inhibitors are being pursued. Recent human genetic data have provided strong evidence for the involvement of the peripheral nerve sodium channel subtype, Nav1.7, in the signaling of nociceptive information, highlighting the importance of identifying selective Nav1.7 blockers for the treatment of chronic pain. Using a high-throughput functional assay, novel Nav1.7 blockers, namely, the 1-benzazepin-2-one series, have recently been identified. Further characterization of these agents indicates that, in addition to high-affinity inhibition of Nav1.7 channels, selectivity against the Nav1.5 and Nav1.8 subtypes can also be achieved within this structural class. The most potent, nonselective member of this class of Nav1.7 blockers has been radiolabeled with tritium. [3H]BNZA binds with high affinity to rat brain synaptosomal membranes (Kd = 1.5 nM) and to membranes prepared from HEK293 cells stably transfected with hNav1.5 (Kd = 0.97 nM). In addition, and for the first time, high-affinity binding of a radioligand to hNav1.7 channels (Kd = 1.6 nM) was achieved with [3H]BNZA, providing an additional means for identifying selective Nav1.7 channel inhibitors. Taken together, these data suggest that members of the novel 1-benzazepin-2-one structural class of Nav1 blockers can display selectivity toward the peripheral nerve Nav1.7 channel subtype, and with appropriate pharmacokinetic and drug metabolism properties, these compounds could be developed as analgesic agents.
Antisense Has Growing Pains (1995)

Antisense compounds once seemed the answer to drug designers' dreams: molecules precisely tailoredto block specific genes. But researchers have run into unexpected difficulties, and they are no longer sure what antisense drugs are doing inside the body. Are clinical trials premature?
Proteome characterization and proteomics (2003)

Proteomics in the Postgenomic Age The Tools of Proteomics Proteomic Analysis by Two-Dimensional Polyacrylamide Gel Electrophoresis High Perfoemance Seperations and Mass Spectrometric Methods for High Throughput Proteomics Using Accurate Mass Tags Current Strategie for Quantitative Proteomics Proteome Analysis of Posttranslational Modifications Mapping Prtein Modifications with LC-MS and the SALSA Algorithm Emerging Role of Mass Spectrometry in Structural and Functional Proteomics The Role of Seperation Science in Proteomics Research Proteomics of Membrane Proteins Proteomics in Drug Discovery From Clone to Crystal: Maximizing the Amount of Protein Samples for Structure Determination Proteomics and Bioinformatics
Adaptive soft-input soft-output algorithms for iterative detection with parametric uncertainty (2000)

The soft-input soft-output (SISO) module is the basic building block for established iterative detection (ID) algorithms for a system consisting of a network of finite state machines. The problem of performing ID for systems having parametric uncertainty has received relatively little attention in the open literature. Previously proposed adaptive SISO (A-SISO) algorithms are either based on an oversimplified channel model, or have a complexity that grows exponentially with the observation length N (or the smoothing lag D). In this paper, the exact expressions for the soft metrics in the presence of parametric uncertainty modeled as a Gauss-Markov process are derived in a novel way that enables the decoupling of complexity and observation length. Starting from these expressions, a family of suboptimal (practical) algorithms is motivated, based on forward/backward adaptive processing with linear complexity in N. Previously proposed A-SISO algorithms, as well as existing adaptive hard decision algorithms are interpreted as special cases within this framework. Using a representative application-joint iterative equalization-decoding for trellis-based codes over frequency-selective channels-several design options are compared and the impact of parametric uncertainty on previously established results for ID with perfect channel state information is assessed.
Serum concentrations of tumour necrosis factor alpha in childhood chronic inflammatory bowel disease. (1991)

Serum tumour necrosis factor alpha (TNF alpha) concentrations were measured by enzyme linked immunoadsorbent assay in 31 normal children and during 65 episodes of clinical remission and 54 episodes of relapse in 92 children with chronic inflammatory bowel disease. An appreciable rise in TNF alpha was found only in children in relapse of ulcerative colitis and colonic Crohn's disease. The group of children with small bowel Crohn's disease in relapse did not show increases of TNF alpha above control concentrations, despite an equivalent rise in disease indices. Height velocity was depressed in children with relapse of large bowel Crohn's disease and ulcerative colitis compared with the equivalent condition in remission. The impairment of growth velocity was significantly greater in relapse of large bowel Crohn's disease and ulcerative colitis than in small bowel Crohn's disease alone, although for the subgroups in stage 1 puberty (prepubertal) the differences were not significant. Inadequate growth in chronic inflammatory bowel disease is currently ascribed to inadequate nutrition and TNF alpha may contribute to this through its cachexia inducing effects. It may, in addition, diminish pituitary growth hormone release. These results suggest that production of TNF alpha may be associated with growth failure in relapse of colonic inflammatory bowel disease.
Polyimide-Enhanced Stretchable Interconnects: Design, Fabrication, and Characterization (2011)

This paper discusses the optimization of a stretchable electrical interconnection between integrated circuits in terms of stretchability and fatigue lifetime. The interconnection is based on Cu stripes embedded in a polyimide-enhanced (PI-enhanced) layer. Design-of-experiment (DOE) methods and finite-element modeling were used to obtain an optimal design and to define design guidelines, concerning both stripe and layer dimensions and material selection. Stretchable interconnects with a PI-enhanced layer were fabricated based on the optimized design parameters and tested. In situ experimental observations did validate the optimal design. Statistical analysis indicated that the PI width plays the most important role among the different design parameters. By increasing the PI width, the plastic strain in the Cu stripes is reduced, and thus, the stretchability and fatigue lifetime of the system is increased. The experimental results demonstrate that the PI-enhanced stretchable interconnect enables elongations up to 250% without Cu rupture. This maximum elongation is two times larger than the one in samples without PI enhancement . Moreover, the fatigue life at 30% elongation is 470 times higher.
Determination of long chain fatty acids in human total plasma lipids using gas chromatography (1982)

エスキモー人の疫学調査により魚脂中に多く含まれるω-3の高級多価不飽和脂肪酸の抗血栓,抗動脈硬化作用が注目されている.ヒト血しょう総脂質中の高級脂肪酸を精度よく測定するためにキャピラリーカラムを装着したガスクロマトグラフを用い血しょうよりFolchらの方法で抽出しBF3メタノールでエステル化した試料を分離分析したところ良好な成績が得られた.本法での抽出操作を含めた再現性はアラキドン酸,エイコサペンタエン酸,ドコサヘキサエン酸のいずれについても5%以下と良好で各各のメチルエステルを用いて得られた添加回収率もほぼ100%と良好な値を示した.又検量線もいずれの脂肪酸について良好な成績が得られ,又実際の実験食投与の健常人においても有意の脂肪酸の変動が認められたことから本法は血しょう総脂質中の高級脂肪酸の定量法の一つとして有用と考えられる.
Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation. (1996)

Low milk SCC after calving may increase susceptibility to severe coliform mastitis.
Chronic Traumatic Encephalopathy in Athletes: Progressive Tauopathy After Repetitive Head Injury (2009)

Since the 1920s, it has been known that the repetitive brain trauma associated with boxing may produce a progressive neurological deterioration, originally termed dementia pugilistica, and more recently, chronic traumatic encephalopathy (CTE). We review 48 cases of neuropathologically verified CTE recorded in the literature and document the detailed findings of CTE in 3 profession althletes, 1 football player and 2 boxers. Clinically, CTE is associated with memory disturbances, behavioral and personality changes, parkinsonism, and speech and gait abnormalities. Neuropathologically, CTE is characterized by atrophy of the cerebral hemispheres, medial temporal lobe, thalamus, mammillary bodies, and brainstem, with ventricular dilatation and a fenestrated cavum septum pellucidum. Microscopically, there are extensive tau-immunoreactive neurofibrillary tangles, astrocytic tangles, and spindle-shaped and threadlike neurites throughout the brain. The neurofibrillary degeneration of CTE is distinguished from other tauopathies by preferential involvement of the superficial cortical layers, irregular patchy distribution in the frontal and temporal cortices, propensity for sulcal depths, prominent perivascular, periventricular, and subpial distribution, and marked accumulation of tau-immunoreactive astrocytes. Deposition of beta-amyloid, most commonly as diffuse plaques, occurs in fewer than half the cases. Chronic traumatic encephalopathy is a neuropathologically distinct slowly progressive tauopathy with a clear environmental etiology.
The monomer–dimer equilibrium of stromal cell‐derived factor‐1 (CXCL 12) is altered by pH, phosphate, sulfate, and heparin (2005)

Chemokines, like stromal cell-derived factor-1 (SDF1/CXCL12), are small secreted proteins that signal cells to migrate. Because SDF1 and its receptor CXCR4 play important roles in embryonic development, cancer metastasis, and HIV/AIDS, this chemokine signaling system is the subject of intense study. However, it is not known whether the monomeric or dimeric structure of SDF1 is responsible for signaling in vivo. Previous structural studies portrayed the SDF1 structure as either strictly monomeric in solution or dimeric when crystallized. Here, we report two-dimensional NMR, pulsed-field gradient diffusion and fluorescence polarization measurements at various SDF1 concentrations, solution conditions, and pH. These results demonstrate that SDF1 can form a dimeric structure in solution, but only at nonacidic pH when stabilizing counterions are present. Thus, while the previous NMR structural studies were performed under acidic conditions that strongly promote the monomeric state, crystallographic studies used nonacidic buffer conditions that included divalent anions shown here to promote dimerization. This pH-sensitive aggregation behavior is explained by a dense cluster of positively charged residues at the SDF1 dimer interface that includes a histidine side chain at its center. A heparin disaccharide shifts the SDF1 monomer-dimer equilibrium in the same manner as other stabilizing anions, suggesting that glycosaminoglycan binding may be coupled to SDF1 dimerization in vivo.
<title>Scatterometry for CD measurements of etched structures</title> (1996)

Scatterometry, the characterization of periodic structures via diffracted light analysis, has been shown to be a versatile technique for measuring critical dimensions in photoresist as small as 0.160 micrometer. Rapid, non-destructive and inexpensive, scatterometry has the potential to be applied to other microlithographic features as well. This paper discusses applications of scatterometry in the measurement of etched sub-um poly-Si line/space patterns. Since etched features represent the final dimensions of a finished product, the characterization of such features is important. Initial attempts at measuring the etched linewidth and height using scatterometry assumed the sidewalls were perfectly vertical. Although results from these two parameter predictions were good, our measurement algorithms suggested that the etch profiles were not square. Thus, sidewall angle was left as an unknown in our model and three parameter predictions were made. These improved results from measuring the linewidth, height and sidewall angle are presented, and comparisons to SEM measurements of the same samples are made. Finally, experiments to determine the repeatability of the scatterometer for measuring etched features were performed. Results show that the repeatability of the instrument, for both static and dynamic measurements of nominal 0.25 micrometer structures, is sub-nanometer for all parameters measured; the 3(sigma) repeatability for static CD measurements is 0.63 nm, and for dynamic measurements is 0.78 nm.
Bluetooth End to End (2002)

From the Book:
PrefaceIn today's mobile society, the need to connect communications and networking devices together with a physical cable is becoming more and more of a nuisance. With the emergence of the digital cellular telephone and handheld (and highly portable) computing devices, users are looking for a way to connect to their local network, the Internet, or even their own desktop computer from wherever they might be. Several emerging technologies can create a wireless world that supports this kind of freedom @md freedom from wires; freedom from land-based telephone systems; and the freedom to access data truly anytime, anywhere.
The Bluetooth technology, which was born from the cellular industry, is only one of the new cable-replacement technologies. Bluetooth, funny name and all, is being developed to provide mobile users with the levels of freedom they desire. Bluetooth technology is certainly a key part of creating what is being called The Mobile Society. In a Bluetooth world, you can connect your handheld device @md be it a cell phone, a notebook computer, or a personal digital assistant (PDA) @md to your network or even to other Bluetooth-enabled devices to create ad hoc, peer-to-peer networks, using wireless technology. If you and a friend want to share data from one Palm Pilot to another, you don't need to hook up with a link cable. Bluetooth technology enables you and your friend to connect up over a wireless link that is automatically configured.
In this book, we offer many other examples of how Bluetooth can be put to use. We provide you with both a general overview of the Bluetooth technology and its applications as well as a more technical, but not too technical, look at the components and how Bluetooth operates to support its applications.
Transport of hydrophobized fragments of antibodies through the blood–brain barrier (1995)

Hydrophobized and non-hydrophobized Fab fragments of human antibodies against gliofibrillar acid protein (GFAP) and brain specific α2-glycoprotein (α2GP) were used to study their penetration through the blood–brain barrier (BBB). These Fab fragments were modified by stearoyl chloride in reversed micelles of aerosol OT in octane (one or two fatty acid residues attached to protein molecule). Modified and non-modified 125I-labelled Fab fragments were intracardially administered to rats. The amount of label accumulated in brain was 55% higher than the total amount in all other organs. In contrast, non-hydrophobized Fab fragments did not penetrate through the BBB. We assume that the artificial hydrophobization of Fab fragments can increase their capability to penetrate through the cell membranes and, in particular, the BBB.
Signal and noise in picked reflection times from partial‐stack seismic data (1988)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 1988Signal and noise in picked reflection times from partial‐stack seismic dataAuthors: Leigh WoodMilo M. BackusLeigh WoodUnocal Science & Technology and Milo M. BackusUniversity of Texas at Austinhttps://doi.org/10.1190/1.1892307 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1892307FiguresReferencesRelatedDetails SEG Technical Program Expanded Abstracts 1988ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 1988 Pages: 1359 publication data© 1988 Copyright © 1988 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished Online: 10 Feb 2005 CITATION INFORMATION Leigh Wood and Milo M. Backus, (1988), "Signal and noise in picked reflection times from partial‐stack seismic data," SEG Technical Program Expanded Abstracts : 775-779. https://doi.org/10.1190/1.1892307 Plain-Language Summary PDF DownloadLoading ...
Stationary‐phase regulation of the Saccharomyces cerevisiaeSOD2 gene is dependent on additive effects of HAP2/3/4/5‐ and STRE‐binding elements (1997)

SOD2, encoding manganese superoxide dismutase (MnSOD), is essential for stationary-phase survival of yeast cells. In addition, stationary-phase cells are more resistant to oxidative stress than exponential-phase cells. The use of a SOD2::lacZ fusion construct in this study shows that transcription of SOD2 increases 6.5-fold as cells enter stationary phase in rich, glucose medium. The increase in SOD2 expression appears to be due to two phenomena-the switch to a non-fermentable carbon source and nutrient limitation. Analysis of SOD2 transcription in mutant Saccharomyces cerevisiae strains showed that the gene was negatively regulated by intracellular cAMP levels which decrease as cells enter stationary phase. Mutation of 'stress-responsive' (STRE) elements in the SOD2 promoter which respond to cAMP levels resulted in the loss of cAMP-dependent expression but only partially reduced the increase in expression as cells entered stationary phase. A putative Yap1p-binding site was found to be inactive and mutation of YAP1 had no effect on the STRE-mediated expression. To fully eliminate the stationary-phase response, it was necessary to mutate a HAP2/3/4/5 complex binding site in addition to the STRE elements. It is postulated that the effects of the STRE sites and the HAP2/3/4/5 complex binding site are additive.
Ultrasonic relaxations in aqueous solutions of alcohols and the balance between hydrophobicity and hydrophilicity of the solutes (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUltrasonic relaxations in aqueous solutions of alcohols and the balance between hydrophobicity and hydrophilicity of the solutesSadakatsu Nishikawa and Masayuki UedaCite this: J. Phys. Chem. 1993, 97, 14, 3539–3544Publication Date (Print):April 1, 1993Publication History Published online1 May 2002Published inissue 1 April 1993https://doi.org/10.1021/j100116a017RIGHTS & PERMISSIONSArticle Views72Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (623 KB) Get e-Alerts