gebhart/patrepeval huggingface dataset:
Variant-specific surface proteins of Giardia lamblia are zinc-binding proteins. (1993)

Giardia lamblia undergoes surface antigenic variation. The variant-specific surface proteins (VSPs) are a distinct family of cysteine-rich proteins. Characteristically, cysteine residues occur mostly as CXXC tetrapeptides. Four of the reported five VSPs contain a putative metal-binding domain that resembles other metal-binding motifs; the fifth is closely related but lacks an essential histidine. Three different native VSPs bound Zn2+. Co2+, Cu2+, and Cd2+ inhibited Zn2+ binding. Analysis of recombinant VSP fusion proteins showed that the putative binding motif bound Zn2+. Surprisingly, peptide fragments from other regions of the VSP contain numerous CXXCXnCXXC motifs that also bound Zn2+. Analysis of deduced amino acid sequences showed well-conserved CXXC spacing in three out of five VSPs, suggesting conservation of structure despite amino acid sequence divergence. The function of VSPs is unknown, but by binding Zn2+ or other metals in the intestine, VSPs may contribute to Zn2+ malnutrition or inhibition of metal-dependent intestinal enzymes, which would lead to malabsorption, a well-known consequence of giardiasis.
Antigenic analysis of Campylobacter species and an intracellular Campylobacter-like organism associated with porcine proliferative enteropathies (1989)

Whole-cell and outer membrane preparations of Campylobacter mucosalis, C. hyointestinalis, C. jejuni, and C. coli isolated from porcine intestines were compared with preparations of intracellular Campylobacter-like organisms extracted directly from the lesions of pigs with proliferative enteropathy. By gradient polyacrylamide gel electrophoresis, outer membrane and total protein profiles of C. mucosalis, C. hyointestinalis, C. jejuni, and C. coli were significantly different from each other and from those of the Campylobacter-like organisms. Immunoblotting of these preparations with rabbit antisera or monoclonal antibodies prepared against the intracellular Campylobacter-like organisms showed strong reactions only with a 25,000- to 27,000-molecular-weight component of preparations of the intracellular organisms. Antisera to cultivable Campylobacter species isolates did not react with preparations of intracellular organisms. Isoelectric focusing of sonicated preparations showed protein profile differences and an immune-reactive component in the intracellular organisms with a pI of 4.5. This study suggests that the intracellular Campylobacter-like organism associated with proliferative enteropathy may be a novel bacterium with significant antigenic differences from the Campylobacter species previously associated with the disease.
Machine Learning, Neural and Statistical Classification (1995)

Survey of previous comparisons and theoretical work descriptions of methods dataset descriptions criteria for comparison and methodology (including validation) empirical results machine learning on machine learning.
Effect of denosumab versus zoledronic acid in preventing skeletal-related events in patients with bone metastases by baseline characteristics (2015)

Denosumab was superior to ZA in preventing SREs in patients with bone metastases from advanced cancer, regardless of ECOG PS, bone metastasis number, baseline visceral metastasis presence/absence, and uNTx level.
Heterogeneity of structural relaxation in a particle-suspension system (2007)

We found the mechanism of how a silica-particle–reinforced elastomer after being sheared in oscillation at a strain could produce a spectrum hole or a drop in its dissipation spectrum at that amplitude, but not at other amplitudes. We also found that sequential holding the system at two strains could produce one or two holes depending on the deformation histories. When the second holding strain was higher than the first, the effect of hole burning of only high strain was evident, whereas the effects of hole burning at both strains were revealed when the second strain was lower than the first. This unexpected discovery suggests that the structural relaxations of a particle-suspension system are heterogeneous, and shearing at a fixed strain only affects part of its relaxation spectra.
Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection (1988)

Culture conditions were developed that induce Arabidopsis thaliana (L.) Heynh. root cuttings to regenerate shoots rapidly and at 100% efficiency. The shoots produce viable seeds in vitro or after rooting in soil. A transformation procedure for Arabidopsis root explants based on kanamycin selection was established. By using this regeneration procedure and an Agrobacterium tumor-inducing Ti plasmid carrying a chimeric neomycin phosphotransferase II gene (neo), transformed seed-producing plants were obtained with an efficiency between 20% and 80% within 3 months after gene transfer. F(1) seedlings of these transformants showed Mendelian segregation of the kanamycin-resistance trait. The transformation method could be applied to three different Arabidopsis ecotypes. In addition to the neo gene, a chimeric bar gene conferring resistance to the herbicide Basta was introduced into Arabidopsis. The expression of the bar gene was shown by enzymatic assay.
Thiolation of Chitosan. Attachment of Proteins via Thioether Formation (2005)

Chitosan has a variety of biological functions through conjugating of other compounds to their amino and hydroxyl groups. To further expand applicability of chitosan, we have modified the amino group of chitosan with 2-iminothiolane to bestow thiol groups and obtained about 20% yield, which is equivalent to 913 microequiv SH/g chitosan or 457 nequiv SH/nmol chitosan. Bovine serum albumin (BSA) was reacted with N-(epsilon-maleimidocaproyloxy)sulfosuccinimide ester (sulfo-EMCS), and maleimide-modified BSA (MalN-BSA) was obtained. The yield of sulfo-EMCS addition was 12.8-36.8 mol MalN/mol BSA. When the chitosan-SH was reacted with MalN-BSA via thioether, 97.8% of the maleimide group was reacted, and 37.2% of the SH group was consumed. The remaining SH group was quenched by bromoacetamide. This is the first report of covalent conjugation of a protein to chitosan. Our method should find many applications in developing new chitosan-based biomedical materials containing other components such as growth factors and cell adhesion molecules, known to be crucial to cells. Our thiolated chitosan will facilitate conjugation of such biomedical components to provide new types of materials for tissue engineering.
Quantification of Demodex folliculorum by PCR in rosacea and its relationship to skin innate immune activation (2012)

The aim of this study is to quantify D. folliculorum colonisation in rosacea subtypes and age-matched controls and to determine the relationship between D. folliculorum load, rosacea subtype and skin innate immune system activation markers. We set up a multicentre, cross-sectional, prospective study in which 98 adults were included: 50 with facial rosacea, including 18 with erythematotelangiectatic rosacea (ETR), and 32 with papulopustular rosacea (PPR) and 48 age- and sex-matched healthy volunteers. Non-invasive facial samples were taken to quantify D. folliculorum infestation by quantitative PCR and evaluate inflammatory and immune markers. Analysis of the skin samples show that D. folliculorum was detected more frequently in rosacea patients than age-matched controls (96% vs 74%, P < 0.01). D. folliculorum density was 5.7 times higher in rosacea patients than in healthy volunteers. Skin sample analysis showed a higher expression of genes encoding pro-inflammatory cytokines (Il-8, Il-1b, TNF-a) and inflammasome-related genes (NALP-3 and CASP-1) in rosacea, especially PPR. Overexpression of LL-37 and VEGF, as well as CD45RO, MPO and CD163, was observed, indicating broad immune system activation in patients with rosacea. In conclusion, D. folliculorum density is highly increased in patients with rosacea, irrespective of rosacea subtype. There appears to be an inverse relationship between D. folliculorum density and inflammation markers in the skin of rosacea patients, with clear differences between rosacea subtypes.
Cytokines and depression in cases with fibromyalgia. (2002)

IL-8 may play an important role in the occurrence of pain in FM.
Development of an In Vitro Activity Assay as an Alternative to the Mouse Bioassay for Clostridium botulinum Neurotoxin Type A (2008)

Currently, the only accepted assay with which to detect active Clostridium botulinum neurotoxin is an in vivo mouse bioassay. The mouse bioassay is sensitive and robust and does not require specialized equipment. However, the mouse bioassay is slow and not practical in many settings, and it results in the death of animals. Here, we describe an in vitro cleavage assay for SNAP-25 (synaptosome-associated proteins of 25 kDa) for measuring the toxin activity with the same sensitivity as that of the mouse bioassay. Moreover, this assay is far more rapid, can be automated and adapted to many laboratory settings, and has the potential to be used for toxin typing. The assay has two main steps. The first step consists of immunoseparation and concentration of the toxin, using immunomagnetic beads with monoclonal antibodies directed against the 100-kDa heavy chain subunit, and the second step consists of a cleavage assay targeting the SNAP-25 peptide of the toxin, labeled with fluorescent dyes and detected as a fluorescence resonance energy transfer assay. Our results suggest that the sensitivity of this assay is 10 pg/ml, which is similar to the sensitivity of the mouse bioassay, and this test can detect the activity of the toxin in carrot juice and beef. These results suggest that the assay has a potential use as an alternative to the mouse bioassay for analysis of C. botulinum type A neurotoxin.
Birth Weight and Its Relationship to Size Attained and Relative Fat Distribution at 7 to 12 Years of Age (1996)

The relationship between birth weight and relative subcutaneous fat distribution at school age was considered in 131 boys and 106 girls 7 to 12 years of age. Relative fat distribution at school age was estimated with the ratio of the subscapular to triceps skinfolds (S/T) for the total sample, and with the ratio of the sum of two trunk (subscapular, midaxillary) to the sum of two extremity (triceps, medial calf) skinfolds (T/E) for subsamples of 102 boys and 63 girls. There were no sex differences in the S/T ratio (mm/mm), boys 0.62 +/- 0.15, girls 0.63 +/- 0.18; T/E ratio (mm/mm), boys 0.58 +/- 0.13, girls 0.59 +/- 0.16; and BMI (kg/m2), boys 17.1 +/- 2.4, girls 16.9 +/- 2.2. Second order partial correlations, controlling for age and the BMI or age and sum of skinfolds, between birth weight and the skinfold ratios are, respectively, -0.22 and -0.20 (p < 0.01) for S/T and -0.29 and -0.32 (p < 0.01) for T/E in girls, and -0.18 and -0.17 (p < 0.05) for S/T and -0.06 and -0.6 for T/E in boys. Though low, the correlations suggest that as birth weight decreases proportionally more subcutaneous fat is accumulated on the trunk than on the extremities, more so in females than in males. Results of stepwise multiple regression analyses indicate that birth weight accounts for from 2% to 8% of the variance in relative subcutaneous fat distribution at school age.
Psychotherapists' Duty to Warn: Ten Years After Tarasoff (1985)

This Comment discusses the Tarasoff decisions and subsequent cases defining the scope of the psychotherapists' duty to protect persons other than their patients. It examines the rationale behind A.B. 2900, and assesses the bill's effect upon the Tarasoff-related objections it addresses. In spite of the Governor's veto of A.B. 2900, there is a need for statutory guidelines to clearly and equitably define the scope of the psychotherapists' duty to protect. This Comment proposes a model statute that attempts to strike a favorable balance among the complex, overlapping interests of psychotherapists, patients, and the public.
[The role of opioid antagonists in the treatment of obesity. Results of a clinical trial with naltrexone]. (1991)

On the basis of literature data relative to the alteration of beta-endorphin tone in obese subjects, we conducted a clinical trial employing Naltrexone (a receptorial antagonist of endogen opioids) in order to evaluate its efficacy in increasing the compliance of these subjects on dietary treatment. The drug dosage was 50 mg/daily (100 mg/die). The clinical study was carried out following the double-blind crossover method for a period of 4 months in a group of 17 obese subjects who also underwent to psychodiagnostic interviews. Significant weight reduction was observed in the 9 patients who carried out the treatment by assuming Naltrexone (kg 4.00 +/- 3.97) rather than with placebo (kg 0.96 +/- 4.95). The drug was subjectively well tolerated and it did not alter the endocrine, metabolic, psychometric, and cardiovascular monitored parameters. The insulin secretion during OGTT did not show significant alterations. Our data emphasize the Naltrexone efficacy in improving the compliance of the obese subjects during dietary treatment.
“Living” Free Radical Polymerization:  A Unique Technique for the Preparation of Controlled Macromolecular Architectures (1997)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT"Living" Free Radical Polymerization: A Unique Technique for the Preparation of Controlled Macromolecular ArchitecturesCraig J. HawkerView Author Information Center for Polymeric Interfaces and Macromolecular Assemblies, IBM Almaden Research Center, 650 Harry Road, San Jose, California, 95120-6099 Cite this: Acc. Chem. Res. 1997, 30, 9, 373–382Publication Date (Web):September 11, 1997Publication History Received18 March 1997Published online11 September 1997Published inissue 1 September 1997https://pubs.acs.org/doi/10.1021/ar960248mhttps://doi.org/10.1021/ar960248mresearch-articleACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views3136Altmetric-Citations340LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Copolymers,Free radicals,Living polymerization,Physical and chemical properties,Polymers Get e-Alerts
Pathogenesis of the Human Opportunistic PathogenPseudomonas aeruginosa PA14 in Arabidopsis (2000)

The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 is a multihost pathogen that can infect Arabidopsis. We found that PA14 pathogenesis in Arabidopsis involves the following steps: attachment to the leaf surface, congregation of bacteria at and invasion through stomata or wounds, colonization of intercellular spaces, and concomitant disruption of plant cell wall and membrane structures, basipetal movement along the vascular parenchyma, and maceration and rotting of the petiole and central bud. Distinctive features of P. aeruginosa pathogenesis are that the surface of mesophyll cell walls adopt an unusual convoluted or undulated appearance, that PA14 cells orient themselves perpendicularly to the outer surface of mesophyll cell walls, and that PA14 cells make circular perforations, approximately equal to the diameter of P. aeruginosa, in mesophyll cell walls. Taken together, our data show that P. aeruginosa strain PA14 is a facultative pathogen of Arabidopsis that is capable of causing local and systemic infection, which can result in the death of the infected plant.
Liposomes as vehicles for vaccines. (1980)

Lipid A from Shigella flexneri LPS, or acylated derivatives of muramyl dipeptide (MDP), were incorporated into the lipid bilayer of liposomes to enhance the adjuvanticity of the liposomes in rabbits. Liposomes containing lipid A induced antibodies against lipid A, phosphocholine, phosphatidylcholine, and sphingomyelin. Lipid A was resolved into eight fractions, some of which did, and others of which did not, induce antibodies against liposomes. Anti-liposome antibodies also were induced (in the absence of liposomes) by complete Freund's adjuvant, and by acid treated bacterial cells coated with lipid A, but were not induced either by lipid A or liposomes alone, or by liposomes containing acylated MDP. We tested the liposomes for the ability to enhance the immunogenicity of a protein antigen. The antigen consisted of liposomes having acylated MDP and ganglioside GM1 (the receptor for cholera toxin, or CT) in the lipid membrane, and CT bound to the outer surface of the liposomes. The liposomal antigen (having surface-bound CT) produced a much greater anti-CT titer than that obtained by injection of CT alone. We conclude that liposomes containing only phosphatidylcholine, cholesterol, and dicetyl phosphate are poorly immunogenic, but that antibodies against them can be induced by inclusion of lipid A. Liposomes that are appropriately formulated can strongly enhance the immunogenicity of a liposome-bound protein antigen.
Database on lead-free solders (2002)

The worldwide movement in the electronics industry to replace lead-tin eutectic solders with lead-free solders creates a need for critical data on the industry's new lead-free solder compositions for these design and reliability models. Our team is working with the NEMI Lead-free Alloy Task Group to develop a comprehensive database.
Androgens and the skeleton. (2005)

Loss of estrogens or androgens causes bone loss by increasing the rate of bone remodeling, and also causes an imbalance between resorption and formation by prolonging the lifespan of osteoclasts and shortening the lifespan of osteoblasts. Conversely, treatment with androgens, as well as estrogens, maintains cancellous bone mass and integrity, regardless of age or sex. Both androgens, via the androgen receptor (AR), and estrogens, via the estrogen receptors (ERs) can exert these effects, but the relative contribution of these 2 pathways remains uncertain. Androgens, like estrogens, stimulate endochondral bone formation at the start of puberty, whereas they induce epiphyseal closure at the end of puberty, thus, they have a biphasic effect. Androgen action on the growth plate is, however, clearly mediated via aromatization into estrogens and interaction with ER alpha. Androgens increase, while estrogens decrease radial growth. This differential effect of the sex steroids may be important because bone strength in males seems to be determined by higher periosteal bone formation and, therefore, greater bone dimensions. Experiments in mice suggest that both the AR and ER alpha pathways are involved in androgen action on radial bone growth. ER beta may mediate growth-limiting effects of estrogens in the female but does not seem to be involved in the regulation of bone size in males. In conclusion, androgens may protect men against osteoporosis via maintenance of cancellous bone mass and expansion of cortical bone. This androgen action on bone is mediated by the AR and ER alpha.
Animating the escape response of the sea anemone, Stomphia coccinea from the starfish, Dermasterins imbricata modeled using implicit surfaces (2002)

Many of the most interesting simulations in the biological world have to do with interactions between species. The predator-prey interactions among aquatic organisms are an interesting part of the natural world which has not been seen much in computer animation. This paper explores the interaction between various sea anemones and the starfish Dermasterias imbricata. Although a simulation between a specific sea anemone, Stomphia coccinea to Dermasterias imbricata was created, an approach was taken such that different anemones with minor parameter changes can be used to replace Stomphia coccinea. The animation was created using a parametric key-frame approach using tracks and a hierarchical procedural implicit modeling method. The anemone and starfish were modeled using the Blob-Tree. Using a hierarchical construction, the model is refined locally and deformed globally at arbitrary layers while maintaining the consistency and integrity of surface details. A hierarchy of tracks was used to control the object's motion.
Bokode (2009)

We show a new camera based interaction solution where an ordinary camera can detect small optical tags from a relatively large distance. Current optical tags, such as barcodes, must be read within a short range and the codes occupy valuable physical space on products. We present a new low-cost optical design so that the tags can be shrunk to 3mm visible diameter, and unmodified ordinary cameras several meters away can be set up to decode the identity plus the relative distance and angle. The design exploits the bokeh effect of ordinary cameras lenses, which maps rays exiting from an out of focus scene point into a disk like blur on the camera sensor. This bokeh-code or Bokode is a barcode design with a simple lenslet over the pattern. We show that a code with 15 μm features can be read using an off-the-shelf camera from distances of up to 2 meters. We use intelligent binary coding to estimate the relative distance and angle to the camera, and show potential for applications in augmented reality and motion capture. We analyze the constraints and performance of the optical system, and discuss several plausible application scenarios.
Preparation and properties of dioxomolybdenum alkoxides, phenoxide, and siloxides prepared by the cocondensation reaction of molybdenum trioxide with alkoxy- or (aryloxy)silanes (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and properties of dioxomolybdenum alkoxides, phenoxide, and siloxides prepared by the cocondensation reaction of molybdenum trioxide with alkoxy- or (aryloxy)silanesGyu Shik Kim, David Huffman, and Carroll W. DeKockCite this: Inorg. Chem. 1989, 28, 7, 1279–1283Publication Date (Print):April 1, 1989Publication History Published online1 May 2002Published inissue 1 April 1989https://pubs.acs.org/doi/10.1021/ic00306a015https://doi.org/10.1021/ic00306a015research-articleACS PublicationsRequest reuse permissionsArticle Views249Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts