gebhart/patrepeval huggingface dataset:
Prolonged androgen deprivation leads to downregulation of androgen receptor and prostate-specific membrane antigen in prostate cancer cells (2012)

Emergence of androgen-independent cancer cells during androgen deprivation therapy presents a significant challenge to successful treatment outcomes in prostate cancer. Elucidating the role of androgen deprivation in the transition from an androgen-dependent to an androgen-independent state may enable the development of more effective therapeutic strategies against prostate cancer. Herein, we describe an in vitro model for assessing the effects of continuous androgen-deprivation on prostate cancer cells (LNCaP) with respect to the expression of two prostate-specific markers: the androgen receptor (AR) and prostate-specific membrane antigen (PSMA). Compared with androgen-containing normal growth medium, androgen-deprived medium apparently induced the concomitant downregulation of AR and PSMA over time. Decreased protein levels were confirmed by fluorescence imaging, western blotting and enzymatic activity studies. In contrast to the current understanding of AR and PSMA in prostate cancer progression, our data demonstrated that androgen-deprivation induced a decrease in AR and PSMA levels in androgen-sensitive LNCaP cells, which may be associated with the development of more aggressive disease-state following androgen deprivation therapy.
Prefrontal and Cerebellar Abnormalities in Major Depression: Evidence from Oculomotor Studies (1998)

These results provide quantitative documentation of significant disturbances in neurophysiological processes subserved by prefrontal cortex and the cerebellar vermis during episodes of major depression.
Ironing Iron Out in Parkinson's Disease and Other Neurodegenerative Diseases with Iron Chelators: A Lesson from 6‐Hydroxydopamine and Iron Chelators, Desferal and VK‐28 (2004)

In Parkinson's disease (PD) and its neurotoxin-induced models, 6-hydroxydopamine (6-OHDA) and N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), significant accumulation of iron occurs in the substantia nigra pars compacta. The iron is thought to be in a labile pool, unbound to ferritin, and is thought to have a pivotal role to induce oxidative stress-dependent neurodegeneration of dopamine neurons via Fenton chemistry. The consequence of this is its interaction with H(2)O(2) to generate the most reactive radical oxygen species, the hydroxyl radical. This scenario is supported by studies in both human and neurotoxin-induced parkinsonism showing that disposition of H(2)O(2) is compromised via depletion of glutathione (GSH), the rate-limiting cofactor of glutathione peroxide, the major enzyme source to dispose H(2)O(2) as water in the brain. Further, radical scavengers have been shown to prevent the neurotoxic action of the above neurotoxins and depletion of GSH. However, our group was the first to demonstrate that the prototype iron chelator, desferal, is a potent neuroprotective agent in the 6-OHDA model. We have extended these studies and examined the neuroprotective effect of intracerebraventricular (ICV) pretreatment with the prototype iron chelator, desferal (1.3, 13, 134 mg), on ICV induced 6-OHDA (250 micro g) lesion of striatal dopamine neurons. Desferal alone at the doses studied did not affect striatal tyrosine hydroxylase (TH) activity or dopamine (DA) metabolism. All three pretreatment (30 min) doses of desferal prevented the fall in striatal and frontal cortex DA, dihydroxyphenylacetic acid, and homovalinic acid, as well as the left and right striatum TH activity and DA turnover resulting from 6-OHDA lesion of dopaminergic neurons. A concentration bell-shaped neuroprotective effect of desferal was observed in the striatum, with 13 micro g being the most effective. Neither desferal nor 6-OHDA affected striatal serotonin, 5-hydroxyindole acetic acid, or noradrenaline. Desferal also protected against 6-OHDA-induced deficit in locomotor activity, rearing, and exploratory behavior (sniffing) in a novel environment. Since the lowest neuroprotective dose (1.3 micro g) of desferal was 200 times less than 6-OHDA, its neuroprotective activity may not be attributed to interference with the neurotoxin activity, but rather iron chelation. These studies led us to develop novel brain-permeable iron chelators, the VK-28 series, with iron chelating and neuroprotective activity similar to desferal for ironing iron out from PD and other neurodegenerative diseases, such as Alzheimer's disease, Friedreich's ataxia, and Huntington's disease.
A Framework for Feature Selection for Background Subtraction (2006)

Background subtraction is a widely used paradigm to detect moving objects in video taken from a static camera and is used for various important applications such as video surveillance, human motion analysis, etc. Various statistical approaches have been proposed for modeling a given scene background. However, there is no theoretical framework for choosing which features to use to model different regions of the scene background. In this paper we introduce a novel framework for feature selection for background modeling and subtraction. A boosting algorithm, namely RealBoost, is used to choose the best combination of features at each pixel. Given the probability estimates from a pool of features calculated by Kernel Density Estimate (KDE) over a certain time period, the algorithm selects the most useful ones to discriminate foreground objects from the scene background. The results show that the proposed framework successfully selects appropriate features for different parts of the image.
Compatibility of Imidacloprid with Fungicides as a Seed-Treatment Control of Russian Wheat Aphid (Homoptera: Aphididae) and Effect on Germination, Growth, and Yield of Wheat and Barley (1993)

Imidacloprid insecticide, applied as a seed treatment, singly or in combination with fungicides (carboxin-thiram, triadimenol-captan, and tebuconazole-thiram), protected growing plants of wheat and barley from developing infestations of Russian wheat aphid, Diuraphis noxia (Mordvilko) for 27-85 d after planting based on four greenhouse and four field trials. There were no compatibility problems in insecticide performance by combining imidacloprid with fungicides on the seed. Imidacloprid significantly reduced plant damage by D. noxia in all trials and increased yields in three of three field trials where plots were entirely infested. As a seed treatment, imidacloprid offers an environmentally safer approach to the control of D. noxia than is possible with wide-spectrum aerial or in-furrow granular insecticide treatments.
Factorial Hidden Markov Models for Speech Recognition: Preliminary Experiments (1997)

The Cambridge Research Laboratory was founded in 1987 to advance the state of the art in both core computing and human-computer interaction, and to use the knowledge so gained to support the Company’s corporate objectives. We believe this is best accomplished through interconnected pursuits in technology creation, advanced systems engineering, and business development. We are actively investigating scalable computing; mobile computing; vision-based human and scene sensing; speech interaction; computer-animated synthetic persona; intelligent information appliances; and the capture, coding, storage, indexing, retrieval, decoding, and rendering of multimedia data. We recognize and embrace a technology creation model which is characterized by three major phases: Freedom: The life blood of the Laboratory comes from the observations and imaginations of our research staff. It is here that challenging research problems are uncovered (through discussions with customers, through interactions with others in the Corporation, through other professional interactions, through reading, and the like) or that new ideas are born. For any such problem or idea, this phase culminates in the nucleation of a project team around a well articulated central research question and the outlining of a research plan. Focus: Once a team is formed, we aggressively pursue the creation of new technology based on
Mapping Complex Genetic Traits in Humans: New Methods Using a Complete RFLP Linkage Map (1986)

It has been clear since the rediscovery of Mendel that humans obey laws of heredity identical with those of other organisms. The central features of Mendelism were observable in humans by following simply inherited common traits, including some diseases. However, the systematic study of human heredity using the standard concepts (complementation and recombination, tests of epistasis, etc.) has been infeasible in humans for two reasons: (1) because Homo sapiens is not an experimental animal that can be manipulated at will and (2) because few genetic markers had been found that were heterozygous often enough to allow random matings to be informative. The advent of recombinant DNA technology led to the suggestion (Botstein et al. 1980) that common polymorphisms in DNA sequence (conveniently observed as restriction fragment length polymorphisms, or RFLPs) could be used as generally informative genetic markers allowing the systematic study of heredity in humans, including the construction of...
Kinetics and mechanism of complex-formation reactions of ammonia and methylamine with copper(II) complexes in aqueous solution (1994)

The complex-formation kinetics of copper(II) complexes with ammonia and methylamine was investigated by the temperature-jump relaxation technique. At 2 mol dm–3 ionic strength the forward rate constants (dm3 mol–1 s–1) for the studied reactions are: [Cu(NH3)]2++ NH3⇌[Cu(NH3)2]2+, (1.2 ± 0.1)× 108(7 °C); [Cu(ida)]+ NH3⇌[Cu(ida)(NH3)], (3.7 ± 0.3)× 108; [Cu(ida)]+ NMeH2⇌[Cu(ida)(NMeH2)], (8.7 ± 0.3)× 107; [Cu(mida)]+ NMeH2⇌[Cu(mida)(NMeH2)]. (5.4 ± 0.3)× 107(25 °C); ida = iminodiacetate, mida =N-methyliminodiacetate. The results presented and those from previous literature strongly suggest that specific interactions between the co-ordinated groups and the central metal ion do not modify the kinetics and mechanism of the complex formation. The kinetic data are consistent with the Eigen–Wilkins mechanism.
Flow effect on lesion formation in RF cardiac catheter ablation (2001)

This study investigated the flow effect on the lesion formation during radio-frequency cardiac catheter ablation in temperature-controlled mode. The blood flow in heart chambers carries heat away from the endocardium by convection. This cooling effect requires more power from the ablation generator and causes a larger lesion. We set up a flow system to simulate the flow inside the heart chamber. We performed in vitro ablation on bovine myocardium with three different flow rates (0 L/min, 1 L/min and 3 L/min) and two target temperatures (60 degrees C and 80 degrees C). During ablation, we also recorded the temperatures inside the myocardium with a three-thermocouple temperature probe. The results show that lesion dimensions (maximum depth, maximum width and lesion volume) are larger in high flow rates (p<0.01). Also, the temperature recordings show that the tissue temperature rises faster and reaches a higher temperature under higher flow rate.
Effects of Probiotics and Synbiotics on Obesity, Insulin Resistance Syndrome, Type 2 Diabetes and Non-Alcoholic Fatty Liver Disease: A Review of Human Clinical Trials (2016)

The use of probiotics and synbiotics in the prevention and treatment of different disorders has dramatically increased over the last decade. Both probiotics and synbiotics are well known ingredients of functional foods and nutraceuticals and may provide beneficial health effects because they can influence the intestinal microbial ecology and immunity. The present study reviews the effects of probiotics and synbiotics on obesity, insulin resistance syndrome (IRS), type 2 diabetes (T2D) and non-alcoholic fatty liver disease (NAFLD) in human randomized clinical trials. Select probiotics and synbiotics provided beneficial effects in patients with obesity, mainly affecting the body mass index and fat mass. Some probiotics had beneficial effects on IRS, decreasing the cell adhesion molecule-1 levels, and the synbiotics decreased the insulin resistance and plasma lipid levels. Moreover, select probiotics improved the carbohydrate metabolism, fasting blood glucose, insulin sensitivity and antioxidant status and also reduced metabolic stress in subjects with T2D. Some probiotics and synbiotics improved the liver and metabolic parameters in patients with NAFLD. The oral intake of probiotics and synbiotics as co-adjuvants for the prevention and treatment of obesity, IRS, T2D and NAFLD is partially supported by the data shown in the present review. However, further studies are required to understand the precise mechanism of how probiotics and synbiotics affect these metabolic disorders.
Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer (2003)

The ability to transfect cells with native genometic DNA and engineered gene/vector constructs has played a leading role in accelerating our understanding of gene regulation and function. Yet, despite significant diversification of gene delivery strategies, efforts to expand the repertory of cell types amenable to DNA-mediated gene transfer continue to stumble over a recurrent obstacle—namely, the sensitivity that recipient cells often manifest towards the gene transfer procedure itself (,). To minimize the likelihood of cytopathic effects engendered by chemical and physical methods investigators, more and more, are adopting low toxicity synthetic polymers and ligand-mediated strategies to dispatch copious amounts of exogenous DNA to the surfaces of target cells (, , , , , , , ).
Postsurgical Assessment and Long-term Safety of Recombinant Adeno-Associated Virus–Mediated Gene Transfer Into the Retinas of Dogs and Primates (2005)

These short- and long-term studies on rAAV transgene expression using large animals are encouraging for the prospects of ocular gene therapy applications in humans.
Differential expression of estrogen, progesterone, and epidermal growth factor receptors in normal, benign, and malignant human breast tissues using dual staining immunohistochemistry. (1994)

Distribution of estrogen (ER), progesterone (PR) receptors, and epidermal growth factor (EGF) receptors was assayed by dual staining immunohistochemistry on 28 selected cytosolic ER-positive breast carcinomas and 22 nonmalignant breast tissues. ER-positive tumor cells were detected in 26 (93%) and EGF receptor positive tumor cells were detected in 7 (25%) carcinomas. In five tumors both ER and EGF receptors were detected but localized in distinct tumor cells. Only in one case of ductal carcinoma in situ co-expression was observed in a subset of tumor cells. In contrast, simultaneous expression of ER/PR and EGF receptors was observed in non-neoplastic ductal remnants in the majority of the carcinomas and the fibroadenomas. In addition, double-positive cells were occasionally detected in luminal epithelial cells of normal breast tissue and mastopathies. This study shows that ER/PR and EGF receptors in breast tumor cells are inversely related at the single cell level. However, demonstration of ER/PR and EGF receptors in individual normal luminal cells shows that expression is not mutually exclusive.
Friends, not foes: Magnetoencephalography as a tool to uncover brain dynamics during transcranial alternating current stimulation (2015)

Brain oscillations are supposedly crucial for normal cognitive functioning and alterations are associated with cognitive dysfunctions. To demonstrate their causal role on behavior, entrainment approaches in particular aim at driving endogenous oscillations via rhythmic stimulation. Within this context, transcranial electrical stimulation, especially transcranial alternating current stimulation (tACS), has received renewed attention. This is likely due to the possibility of defining oscillatory stimulation properties precisely. Also, measurements comparing pre-tACS with post-tACS electroencephalography (EEG) have shown impressive modulations. However, the period during tACS has remained a blackbox until now, due to the enormous stimulation artifact. By means of application of beamforming to magnetoencephalography (MEG) data, we successfully recovered modulations of the amplitude of brain oscillations during weak and strong tACS. Additionally, we demonstrate that also evoked responses to visual and auditory stimuli can be recovered during tACS. The main contribution of the present study is to provide critical evidence that during ongoing tACS, subtle modulations of oscillatory brain activity can be reconstructed even at the stimulation frequency. Future tACS experiments will be able to deliver direct physiological insights in order to further the understanding of the contribution of brain oscillations to cognition and behavior.
Drug ratio–dependent antitumor activity of irinotecan and cisplatin combinations in vitro and in vivo (2009)

Irinotecan and cisplatin are two established anticancer drugs, which together constitute an effective combination for treating small-cell lung cancer. We investigated whether the efficacy of this combination could be improved by controlling drug ratios following in vivo administration. Irinotecan and cisplatin combinations were evaluated systematically for drug ratio-dependent synergy in vitro using a panel of 20 tumor cell lines. In vitro screening informatics on drug ratio-dependent cytotoxicity identified a consistently antagonistic region between irinotecan/cisplatin molar ratios of 1:2 to 4:1, which was bordered by two synergistic regions. Liposomal co-formulations of these two agents were developed that exhibited plasma drug half-lives of approximately 6 hours and maintained a fixed drug ratio for more than 24 hours. Drug ratio-dependent antitumor activity was shown in vivo for these liposome formulations, and irinotecan/cisplatin ratios between 5:1 and 10:1 were identified as therapeutically optimal. The relationship between irinotecan/cisplatin ratio and in vivo efficacy was consistent with in vitro drug ratio dependency results. Superior antitumor activity was observed for the liposome-encapsulated 7:1 molar ratio of irinotecan/cisplatin (designated CPX-571) compared with the free-drug cocktail in all models tested. Further efficacy studies in a range of human tumor xenografts, including an irinotecan-resistant model, showed that both liposomal agents contributed to the overall efficacy in a manner consistent with in vivo synergy. These results show the ability of drug delivery technology to enhance the therapeutic activity of irinotecan/cisplatin combination treatment by maintaining synergistic ratios in vivo. CPX-571, a fixed-ratio formulation of irinotecan and cisplatin, is a promising candidate for clinical development.
Variation der Ringgröße von Epothilonen – Totalsynthese von [14]-, [15]-, [17]- und [18]Epothilon A (1998)

Kaum tolerant gegenüber Änderungen der Ringgröße ist das Pharmakophor des Epothilons A: Die 14-, 15-, 17- und 18gliedrigen Ringanaloga 1 (n = 1, 2, 4 bzw. 5) von Epothilon A und deren Desoxy-Verbindungen weisen im Tubulin-Polymerisations-Assay mit Ausnahme von [18]Desoxyepothilon A nur geringe Aktivitäten auf. Dies unterstreicht einmal mehr die hohe Spezifität des Tubulin-Rezeptors.
BCR-ABL mediates arsenic trioxide-induced apoptosis independently of its aberrant kinase activity. (2000)

In the prechemotherapy era arsenic derivatives were used for treatment of chronic myelogenous leukemia, a myeloproliferative disorder characterized by the t(9;22) translocation, the Philadelphia chromosome (Ph+). In acute promyelocytic leukemia response to arsenic trioxide (As2O3) has been shown to be genetically determined by the acute promyelocytic leukemia-specific t(15;17) translocation product PML/RARalpha. Hence, we reasoned that As2O3 might have a selective inhibitory effect on proliferation of BCR-ABL-expressing cells. Here, we report that: (a) As2O3 induced apoptosis in Ph+ but not in Ph- lymphoblasts; (b) enforced expression of BCR-ABL in U937 cells dramatically increased the sensitivity to As2O3; (c) the effect of As2O3 was independent of BCR-ABL kinase activity; and (d) As2O3 reduced proliferation of chronic myelogenous leukemia blasts but not of peripheral CD34+ progenitors. In summary, these data establish As2O3 as a tumor cell-specific agent, making its clinical application in Ph+ leukemia feasible.
Transaction support in read optimized and write optimized file systems (1990)

This paper provides a comparative analysis of five implementations of transaction support. The first of the methods is the traditional approach of implementing transaction processing within a data manager on top of a read optimized file system. The second also assumes a traditional file system but embeds transaction support inside the file system. The third model considers a traditional data manager on top of a write optimized file system. The last two models both embed transaction support inside a write optimized file system, each using a different logging mechanism. Our results show that in a transaction processing environment, a write optimized file system often yields better performance than one optimized for reads. In addition, we show that file system embedded transaction managers can perform as well as data managers when transaction throughput is limited by I/O bandwidth. Finally, even when the CPU is the critical resource, the difference in performance between a data manager an...
Idebenone, green tea, and Coffeeberry® extract: new and innovative antioxidants (2007)

The use of topical antioxidants is gaining favor among dermatologists because of their broad biologic activity. Many are not only antioxidants but also have antiinflammatory and anticarcinogenic activities. Thus for dermatologists these cosmeceuticals have many potential applications. In general, topical antioxidants exert their effects by down-regulating free radical mediated pathways that damage skin. The present study will describe the science behind some of the newest topical antioxidants and outline how they can be used as part of a comprehensive skin care regimen.
The Dark Side of Mast Cell–Targeted Therapy in Prostate Cancer (2012)

Tumor development requires accomplices among white blood cells. Other than macrophages, mast cells have been observed to support the outgrowth of certain neoplasias because of their proangiogenic properties. In some tumor settings, however, mast cells may have a protective role, exerted by their proinflammatory mediators. In prostate cancer, no conclusive data on mast cell function were available. Here, we discuss recent work on the role of mast cells in mouse and human prostate cancer, showing that mast cells can behave alternatively as dangerous promoters, innocent bystanders, or essential guardians of tumors, according to the stage and origin of transformed cells. In particular, mast cells are essential for the outgrowth of early-stage tumors due to their matrix metalloproteinase-9 production, become dispensable in advanced-stage, post-epithelial-to-mesenchymal transition, and are protective against neuroendocrine prostate tumor variants. The common expression of c-Kit by mast cells and neuroendocrine clones suggests a possible competition for the ligand Stem cell factor and offers the chance of curing early-stage disease while preventing neuroendocrine tumors using c-Kit-targeted therapy. This review discusses the implications of these findings on the advocated mast cell-targeted cancer therapy and considers future directions in the study of mast cells and their interactions with other c-Kit-expressing cells.