gebhart/patrepeval huggingface dataset:
Chemical modification of prostaglandin H synthase with diethyl pyrocarbonate (1992)

The role of histidine in catalysis by prostaglandin H synthase has been investigated using chemical modification with diethyl pyrocarbonate (DEPC), an agent that has been found to rather selectively derivatize histidine residues in proteins under mild conditions. Incubation of the synthase apoprotein with DEPC at pH 7.2 resulted in a progressive loss of the capacity for both cyclooxygenase and peroxidase catalytic activities. The kinetics of inactivation of the cyclooxygenase activity were dependent on the concentration of DEPC; a second-order rate constant of 680 M-1 min-1 was estimated for reaction of the apoenzyme at pH 7.2 and 0 degrees C. The kinetics of inactivation of the cyclooxygenase by DEPC exhibited a sigmoidal dependence on the pH, indicating that deprotonation of a group with a pKa of 6.3 was required for inactivation. The presence of the heme prosthetic group slowed, but did not prevent, inactivation by DEPC. The stoichiometry of histidine modification of apoenzyme during inactivation determined from absorbance increases at 242 nm agreed well with the overall stoichiometry of derivatized residues determined with [14C]DEPC, indicating that modification by DEPC was quite selective for histidine residues on the synthase. Although modification of several histidine residues by DEPC was observed, only one of the histidine residues was essential for cyclooxygenase activity. Modification of the holoenzyme with DEPC altered the EPR signal of the hydroperoxide-induced tyrosyl free radical from the wide doublet (35 G, peak-to-trough) found with the native synthase to a narrower singlet (28 G, peak-to-trough) quite like that found in the indomethacin-synthase complex. Reaction of the indomethacin-synthase complex with DEPC was found to increase the cyclooxygenase velocity by 9 times its initial value, to about one-third of the uninhibited value, without displacement of the indomethacin; the peroxidase was significantly inactivated under the same conditions. Histidyl residues in the synthase are thus likely to have important roles not only in cyclooxygenase and peroxidase catalysis but also in the interaction of the synthase with indomethacin.
Focal Cerebral Ischaemia in the Rat: 1. Description of Technique and Early Neuropathological Consequences following Middle Cerebral Artery Occlusion (1981)

A procedure for occluding the stem of the proximal middle cerebral artery of the rat is described. The operation is performed under anaesthesia through a small subtemporal craniectomy. After occlusion, 3 animals were perfused with carbon block and 8 with a FAM fixative (40% formaldehyde, glacial acetic acid, and methanol). The findings were compared with sham-operated animals. Carbon black studies demonstrated an area of impaired perfusion corresponding to the territory of the occluded artery in each animal. Neuropathological studies invariably showed that there was ischaemic brain damage in the cortex and basal ganglia. The frontal cortex was involved in every animal, as was the lateral part of the neostriatum; the sensorimotor and auditory cortex were involved in most animals, whereas the occipital cortex and medial striatum were involved only infrequently. The damage produced by ischaemia could be readily distinguished from the small local lesion seen at the surgical site in sham-operated animals. The ability to produce a consistent focal ischaemic lesion in the rodent brain provides a technical approach that is sufficiently reproducible to enable investigation of the pathophysiology of ischaemia using recently developed autoradiographic and neurochemical methods.
Automatic Recognition of Lyrics in Singing (2010)

The paper considers the task of recognizing phonemes and words from a singing input by using a phonetic hidden Markov model recognizer. The system is targeted to both monophonic singing and singing in polyphonic music. A vocal separation algorithm is applied to separate the singing from polyphonic music. Due to the lack of annotated singing databases, the recognizer is trained using speech and linearly adapted to singing. Global adaptation to singing is found to improve singing recognition performance. Further improvement is obtained by gender-specific adaptation. We also study adaptation with multiple base classes defined by either phonetic or acoustic similarity. We test phoneme-level and word-level n-gram language models. The phoneme language models are trained on the speech database text. The large-vocabulary word-level language model is trained on a database of textual lyrics. Two applications are presented. The recognizer is used to align textual lyrics to vocals in polyphonic music, obtaining an average error of 0.94 seconds for line-level alignment. A query-by-singing retrieval application based on the recognized words is also constructed; in 57&#37; of the cases, the first retrieved song is the correct one.
A Study on the Effects of Turbulence Model and Numerical Scheme on Analysis of the Flow through Airfoil Type Tubular Fan (2003)

Three-dimensional flow through a tubular centrifugal fan with airfoil type blades is analyzed, and the effects of turbulence model and numerical scheme on the results are investigated. Standard k - ε model and k - ω model are tested as turbulence closures. The numerical schemes for convection terms, i.e., Upwind Differencing Scheme (UDS), Mass Weighted Skewed upstream differencing scheme (MWS), Linear Profile Skewed upstream differencing scheme (LPS), and Modified Linear Profile Skewed upstream differencing scheme (MLPS) are also tested, and the performances of these schemes coupled with two turbulence models are evaluated. The static pressure distributions are compared with experimental data obtained in this work, which shows that the k-ε model gives better results than the k-ω model.
Perceptions about quality of life in a school‐based population of adolescents with menorrhagia: implications for adolescents with bleeding disorders (2008)

This study examined the effect of menorrhagia on the quality of life (QOL) of adolescents. We used data from a survey of 45 adolescents. Using the Pictorial Blood Assessment Chart (PBAC) and a PBAC score of > or =100 we identified adolescents likely to have menorrhagia. Our statistical analysis indicated that menorrhagia adversely affects the QOL of adolescents. In this article we discuss the implications of our study and future research avenues.
Neutrophil-Activating Peptide (Interleukin-8) in Colonic Mucosa from Patients with Crohn's Disease (1993)

We considered the role of two neutrophil chemotactic agents (interleukin-8 and leukotriene B4) and of myeloperoxidase (a neutrophil-associated enzyme) in the pathologic condition of Crohn's disease (CD). Serial biopsy samples were taken at different sites in the colon, washed in 0.02 M phosphate-saline buffer, homogenized, and then sonicated. Interleukin-8 levels were significantly increased throughout the colonic mucosa (> 300 pg/mg protein) in patients with CD compared with control groups (< 40 pg/mg protein) (p < or = 0.01). A two- to six-fold increase in leukotriene B4 was also found in CD, whereas mucosal levels of myeloperoxidase were unchanged compared with control subjects. This study demonstrates that interleukin-8 and leukotriene B4 may have an immunologic role in the pathologic condition of CD.
Monitoring and mitigating the voltage collapse problem in the Natal network (2009)

The two main load centers in the Natal region of the Eskom power system in South Africa are susceptible to voltage collapse during stressed operating conditions. Combined, these two load centers carry about 6,700 MW during peak conditions. Potential voltage collapse problems near these load centers can be avoided by shedding load. This paper proposes a system for real-time prediction and mitigation of the voltage collapse problem. The prediction and mitigation system gathers synchrophasor data to compute the power system state. Then the system uses the state and network data to obtain voltage collapse indices, which provide evidence of proximity to voltage collapse and suggest optimal load-shedding schemes.
Video-Based In Situ Tagging on Mobile Phones (2011)

We propose a novel way to augment a real-world scene with minimal user intervention on a mobile phone; the user only has to point the phone camera to the desired location of the augmentation. Our method is valid for horizontal or vertical surfaces only, but this is not a restriction in practice in manmade environments, and it avoids going through any reconstruction of the 3-D scene, which is still a delicate process on a resource-limited system like a mobile phone. Our approach is inspired by recent work on perspective patch recognition, but we adapt it for better performances on mobile phones. We reduce user interaction with real scenes by exploiting the phone accelerometers to relax the need for fronto-parallel views. As a result, we can learn a planar target in situ from arbitrary viewpoints and augment it with virtual objects in real-time on a mobile phone.
Possible involvement of advanced glycation end products in carry-over benefits of atorvastatin in ASCOT-BPLA (2008)

I read with interest the recent study by Sever et al. ,1 which reported that patients originally assigned atorvastatin continued to demonstrate lower event rates in most cardiovascular endpoints after the termination of Lipid Lowering Arm of the ASCOT (ASCOT-LLA) trial, compared with those originally assigned placebo, although LDL-cholesterol levels were almost identical in the two groups during the 2 years extended follow-up period.1 These observations suggest sustained cardioprotective effects of atorvastatin after the cessation of active treatment in at-risk patients with …
Advances in RF MEMS phase shifters from 15 GHz to 35 GHz (2012)

This work reports progress towards building low-loss phase shifters using RF MEMS switch technology for use in communication-on-the-move phased array antennas. Four phase shifters centered at 15 GHz, 21 GHz, 30 GHz and 35 GHz were designed, built, packaged and tested. The average packaged insertion losses were –1.7 dB, –1.8 dB, –2.3 dB and –2.7 dB respectively with RMS phase errors less than 7 degrees in all cases. An innovative liquid crystal polymer packaging technique was used to package these parts at the wafer level. Further reductions in loss are currently being implemented in ongoing work.
Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping (2007)

A potential industrial substrate (liquefied corn starch; LCS) has been employed for successful acetone butanol ethanol (ABE) production. Fermentation of LCS (60 g l(-1)) in a batch process resulted in the production of 18.4 g l(-1) ABE, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l(-1) ABE). A batch fermentation of LCS integrated with product recovery resulted in 92% utilization of sugars present in the feed. When ABE was recovered by gas stripping (to relieve inhibition) from the fed-batch reactor fed with saccharified liquefied cornstarch (SLCS), 81.3 g l(-1) ABE was produced compared to 18.6 g l(-1) (control). In this integrated system, 225.8 g l(-1) SLCS sugar (487 % of control) was consumed. In the absence of product removal, it is not possible for C. beijerinckii BA101 to utilize more than 46 g l(-1) glucose. A combination of fermentation of this novel substrate (LCS) to butanol together with product recovery by gas stripping may economically benefit this fermentation.
The phase-shifting mask II: Imaging simulations and submicrometer resist exposures (1984)

Submicrometer optical lithography is possible with conventional projection cameras when the mask controls the phase of the light at the object plane. Two-dimensional imaging simulations for the Mann 4800 projection camera show that the maximum spatial frequency for 60-percent contrast increases from 640 1/mm to 896 1/mm. The geometrical quality of the images of typical microcircuit patterns was shown to be acceptable for feature sizes of 0.7, 0.6, and 0.5 µ, respectively, and various parameters of the irradiance patterns were calculated. Exposures were made using a high-performance two-layer photoresist system and a mask containing patterns similar to those in the simulation. The phase-shifting mask was shown to increase exposure latitude and to produce a 95-percent yield of 833 1/mm (0.6 µ line and gap) patterns, whereas the transmission mask gave a 7-percent yield. Half micrometer features were patterned with a 22-percent yield using 0.436-µ light.
Variation in Attractiveness of Human Subjects to Malaria Mosquitoes (Diptera: Culicidae) in The Gambia (1993)

During experimental hut trials to assess the efficacy of insecticide-treated bednets against malaria mosquitoes, we observed that human subjects varied consistently in their attractiveness to mosquitoes. Attractiveness was assessed by estimating the numbers of wild Anopheles gambiae Giles mosquitoes entering a hut in which a man was sleeping, and the numbers of human-bloodfed An. gambiae sensu lato collected from each hut each morning. Five trials were carried out at Wali Kunda in rural Gambia during 2.5 yr. During each 6-wk trial a man slept under a bednet in each of the six huts. Morning collections of mosquitoes from the room, enclosed verandas, and window traps of each hut provided estimates of the number of mosquitoes that had entered during the night. Blood meals were analyzed using an ELISA technique to identify those mosquitoes feeding on humans. Specimens were collected by field workers, not the subjects; therefore, sampling was independent of the subjects' ability to catch mosquitoes. Moreover, the trials were designed to measure the relative attractiveness of individual sleepers to mosquitoes, allowing for other sources of variation (i.e., among huts, bednets, nights, and day of the week). Attractiveness of men to mosquitoes differed significantly among individuals as indicated by the consistent differences between the numbers of mosquitoes entering each man's hut and the numbers feeding on each man. However, the two measures of attractiveness were apparently independent of each other: subjects who attracted consistently high numbers of vectors into their hut did not necessarily have high numbers of mosquitoes feeding on them. These findings support the view that some individuals within a community are at greater risk from mosquito-borne pathogens than others.
A comparison of gene organization in the zwf region of the genomes of the cyanobacteria Synechococcus sp. PCC 7942 and Anabaena sp. PCC 7120 (1995)

The region of the genome encoding the glucose-6-phosphate dehydrogenase gene zwf was analysed in a unicellular cyanobacterium, Synechococcus sp. PCC 7942, and a filamentous, heterocystous cyanobacterium, Anabaena sp. PCC 7120. Comparison of cyanobacterial zwf sequences revealed the presence of two absolutely conserved cysteine residues which may be implicated in the light/dark control of enzyme activity. The presence in both strains of a gene fbp, encoding fructose-1,6-bisphosphatase, upstream from zwf strongly suggests that the oxidative pentose phosphate pathway in these organisms may function to completely oxidize glucose 6-phosphate to CO2. The amino acid sequence of fructose-1,6-bisphosphatase does not support the idea of its light activation by a thiol/disulfide exchange mechanism. In the case of Anabaena sp. PCC 7120, the tal gene, encoding transaldolase, lies between zwf and fbp.
Exposure to Rosiglitazone and Fluoxetine in the First Trimester of Pregnancy (2006)

Rosiglitazone is a thiazolidinedione oral hypoglycemic drug that seems to be a promising alternative not only as an oral hypoglycemic agent but also for women with polycystic ovary syndrome. However, information regarding exposure to rosiglitazone in pregnancy is limited to two previous case reports. In the first case, a 35-year-old woman was exposed until the 8th week of pregnancy to 4 mg/day rosiglitazone and to glicazide, acarbose, atorvastatin, spironolactone, hydrochlorothiazide, carbamazepine, thiridazine, amitryptiline, chlordiazepoxide, and pipenzolate bromide (1). The second case was a woman exposed to 4 mg/day rosiglitazone between gestational weeks 13 and 17 (2). The two cases delivered normal babies at gestational weeks 36 and 37, …
Controlled trial of aspirin in cerebral ischemia. (1977)

Adouble-blind trial of aspirin for the treatment of cerebral ischemia was begun in 1972 and continued for 37 months. This was accomplished despite difficulties in controlling a long-term study of a drug which has widespread availability and consumption. The study design, criteria for selection of patients, follow-up surveillance, and methods of data analysis are presented. We report only subjects without carotid surgery before randomization. Patients (178) who had carotid transient ischemic attacks (TIAs) were randomly allocated to aspirin or placebo and followed to determine the incidence of subsequent TIAs,death, cerebral infarction or retinal infarction. Analysis of the first six months of follow-up revealed a statistically significant differential in favar of aspirin when death or cerebral or retinal infarction and the occurrence of TIAs were grouped and considered together as end points. Significance in favor of aspirin treatment was mainly revealed in patients with a history of multiple TIAs and was most evident in those individuals having carotid lesions appropriate to the TIA symptoms. It cannot be inferred from this study that aspirin prevents stroke because when end points were restriced to death or cerebral or retinal infarction, there was no statistically significant differential between the aspirin and placebo treatments.
Monoclonal antibodies in human cancer. (2003)

Mouse, chimeric, humanized and human monoclonal antibodies (MABs) are all in use for treatment of human cancer. Unconjugated antibodies have a complex mechanism of action, dependent on the nature of the target structure. Antibodies can activate the immune system (antibody-dependent cellular cytotoxicity [ADCC], complement-dependent cytotoxicity [CDC], induction of tumor immunity [idiotype network]). ADCC appears to be one of the most important immune effector functions. Antibodies may also induce apoptosis, cell cycle arrest, inhibition of cell proliferation as well as angiogenesis and metastatic spread. For most antibodies there is no clear dose-response relationship in vivo. The effect of antibodies can be enhanced by combination with chemotherapy and/or by agents which activate the immune system. The best therapeutic effect may be obtained if MABs are used early in the course of the disease. Rituximab (anti-CD20) was the first registered MAB for the therapy of follicular lymphoma. Impressive results have been seen in combination with CHOP chemotherapy (cyclophosphamide, doxorubicin, vincristine and prednisone) in follicular and high-grade lymphomas. In other non-Hodgkin's lymphoma subtypes, promising results are also seen in combination with chemotherapy. Trastuzumab (anti-Her2) is a breakthrough in the treatment of breast cancer in combination with chemotherapeutic agents. This antibody is also in clinical testing for adjuvant treatment. Alemtuzumab (anti-CD52) has shown impressive results both in refractory chronic lymphocytic leukemia and as up-front therapy. There are many other antibodies in late stages of testing for registration. Interesting MABs include cetuximab (anti-epidermal growth factor receptor [EGFR]), especially in combination with radiotherapy in head and neck cancer; ABX-EGF (anti-EGFR) in renal carcinoma; bevacizumab (anti-vascular endothelial growth factor) in several solid tumors. Antiepithelial cell adhesion molecule antibodies show promise in combination with chemotherapy in the adjuvant setting of colorectal carcinoma. It is estimated that about 20 antibodies will be in clinical use by the year 2010.
High performance, sub-50nm MOSFETS for mixed signal applications (2006)

We have fabricated and tested sub-50nm gate length nMOSFETs with fT up to 290GHz to assess their suitability for mixed signal applications in the super high frequency (SHF) band, i.e. 3-30GHz. We have also developed an accurate, high frequency (1 -50GHz) model suitable for integration with digital CMOS
Perceiving without Learning: From Spirals to Inside/Outside Relations (1998)

As a benchmark task, the spiral problem is well known in neural net-works. Unlike previous work that emphasizes learning, we approach the problem from a generic perspective that does not involve learning. We point out that the spiral problem is intrinsically connected to the in-side/outside problem. A generic solution to both problems is proposed based on oscillatory correlation using a time delay network. Our simu-lation results are qualitatively consistent with human performance, and we interpret human limitations in terms of synchrony and time delays, both biologically plausible. As a special case, our network without time delays can always distinguish these figures regardless of shape, position, size, and orientation. 1
Purification and Chemical Characterisation of Membrane Glycoproteins from Rat Thymocytes and Brain, Recognised by Monoclonal Antibody MRC OX 2 (1982)

The MRC OX 2 monoclonal antibody recognises antigens present on rat thymocytes, brain, follicular dendritic cells in lymphoid organs, vascular endothelium, some smooth muscle and B-lymphocytes. The OX 2 antigens recognised by this antibody were purified from brain and thymus, by solubilisation with sodium deoxycholate, affinity chromatography with MRC OX 2 antibody and gel filtration. The purified brain and thymocyte OX 2 antigens were glycoproteins with apparent Mr 41000 and 47000 respectively as determined by polyacrylamide gel electrophoresis in sodium dodecyl sulphate. Rabbit antisera raised against the purified antigens were analysed by radioimmunoassay and immunoperoxidase-staining of tissue sections. The brain and thymocyte OX 2 antigens were antigenically very similar to those on the other tissues. This indicates that the unusual pattern of distribution was not the result of fortuitous cross-reaction of the MRC OX 2 antibody, as the rabbit sera would be expected to recognise more determinants on the antigen than that recognised by the monoclonal antibody. The amino acid compositions of brain and thymus OX 2 antigens were very similar but with no distinguishing features. Carbohydrate compositions showed that the OX 2 antigens were highly glycosylated, with brain OX 2 antigen containing 24% and thymocyte OX 2 antigen 33% by weight of carbohydrate. Both OX 2 antigens contained carbohydrate residues typical of structures N-linked to asparagine but lacked galactosamine, indicating the absence of O-linked structures. Thymocyte OX 2 contained higher levels of galactose and sialic acid but less fucose than brain OX 2. Similar differences had been observed for brain and thymocyte Thy-1 antigens and were also observed in pooled glycoproteins purified by lentil affinity chromatography from these tissues, reflecting overall differences in the patterns of glycosylation in the two tissues. The OX 2 antigens showed many similarities to Thy-1 antigens in their odd patterns of distribution, characteristic migration on polyacrylamide gels in sodium dodecyl sulphate, and carbohydrate compositions. It is possible that OX 2 antigens, like Thy-1 antigens, have homologies with immunoglobulin domains. A possible role for OX 2 antigens in cell interactions necessary for tissue organisation is discussed.