gebhart/patrepeval huggingface dataset:
Oral Insulin Product Hexyl-Insulin Monoconjugate 2 (HIM2) in Type 1 Diabetes Mellitus: The Glucose Stabilization Effects of HIM2 (2002)

This study was designed to determine plasma glucose and insulin levels after administration of three escalating doses of the oral insulin product hexyl-insulin monoconjugate 2 (HIM2) in fasting, insulin-deprived adult patients with type 1 diabetes. The study was also designed to assess the safety of the product. Sixteen patients with daily insulin requirements of 27-60 units and glycosylated hemoglobin levels of 5.8-11.1% completed the study. Patients' regular insulin regimens were discontinued at bedtime, and they fasted overnight. Blood glucose levels were stabilized overnight by intravenous insulin infusion. In the morning, intravenous insulin was discontinued 30 min prior to an oral dose of HIM2 (0.6, 0.8, or 1.0 mg/kg). A second oral dose of HIM2 was administered 120 min later. Plasma glucose and insulin levels were measured during a 240-min evaluation period after the first HIM2 dose. Identical HIM2 dosing and study procedures were repeated 1 week later with the same patients. Stable or declining plasma glucose levels were observed on 31 out of a total of 32 dosing days beginning at 20 min after the initial administration of HIM2. After plasma glucose levels declined or were stable for 30 min to 2 h, increases were observed for some patients. However, for the majority of patients (68.8%), plasma glucose levels were <150% of predose levels throughout the postdose evaluation period. Similar results were observed after repeating the study procedures 1 week later. Also, plasma glucose area under the concentration-time curves (AUCs) were inversely correlated with plasma insulin AUCs. HIM2 appeared to be safe and well-tolerated in this study; no episodes of symptomatic hypoglycemia were observed. Thus, HIM2 prevented the expected rise in plasma glucose concentrations in insulin-deprived adult patients with type 1 diabetes. The lack of hypoglycemic events in this exploratory study is encouraging and suggests that there may be less risk of severe hypoglycemia associated with HIM2 when compared with injectable insulin. The promising data in this study support the hypothesis that oral HIM2 reproduces the physiological pathway of insulin secreted by the pancreas - through the portal vein directly to the liver - suggesting a therapeutic advantage in the management of type 1 diabetes mellitus.
Deletion of the Gene Encoding Proprotein Convertase 5/6 Causes Early Embryonic Lethality in the Mouse (2005)

PC5 belongs to the proprotein convertase family and activates precursor proteins by cleavage at basic sites during their transit through the secretory pathway and/or at the cell surface. These precursors include prohormones, proreceptors, growth factors, adhesion molecules, and viral glycoproteins. The Pcsk5 gene encodes two alternatively spliced isoforms, the soluble PC5A and transmembrane PC5B. We have carefully analyzed the expression of PC5 in the mouse during development and in adulthood by in situ hybridization, as well as in mouse tissues and various cell lines by quantitative reverse transcription-PCR. The data show that adrenal cortex and intestine are the richest sources of PC5A and PC5B, respectively. To better define the specific physiological roles of PC5, we have generated a mouse Pcsk5(Delta4)-deficient allele missing exon 4 that encodes the catalytic Asp173. While Delta4/+ heterozygotes were healthy and fertile, genotyping of progeny obtained from Delta4/+ interbreeding indicated that Delta4/Delta4 embryos died between embryonic days 4.5 and 7.5. These data demonstrate that Pcsk5 is an essential gene.
Automatic recording of corneal thickness in vitro. (1976)

An addition to the specular microscope is described which allows it to record the thickness of the excised cornea automatically as a function of time. The focus of the instrument is scanned mechanically through the tissue, and the position of the reflecting surfaces is detected by a photo-electric system and marked on a chart recorder. The system is able to follow thickness changes over periods of many hours and with an accuracy greater than obtainable by manual operation. This system has been helpful in the evaluation of a new medium which considerably extends the useful lifetime of the corneal endothelial fluid pump.
Pyrolysis of sawdust in a conical spouted‐bed reactor with a HZSM‐5 catalyst (2000)

Abstract The effect was studied of using in‐situ catalyst based on a HZSM‐5 zeolite in flash pyrolysis with an inert gas (N 2 ) of sawdust of pinus insignis in a conical spouted‐bed reactor in the 400–500°C range and for a gas residence time of 50 ms. The use of the catalyst increases the yield of gases and decreases the yields of liquid and char. Likewise, the yield of CO 2 decreases, whereas the yield of C 4− hydrocarbons increases (15.9 wt. % at 450°C). The catalyst is efficient for partial deoxygenation of the liquid product.
The Primary Antibody Repertoire Represents a Linked Network of Degenerate Antigen Specificities (2002)

In this study, germline Abs were used to select clones from a random dodecapeptide phage-display library. This revealed a much greater heterogeneity of binders than could be obtained with mutated daughter Abs that presumably had been selected in vivo by nominal Ag during active immune responses. We demonstrate that the pluripotency of germline Abs can subsequently be optimized by binding interactions that correlate with thermodynamic changes indicative of structural adaptations at the interface. This singular feature confers on each Ab a distinct window of Ag specificities, where the entropic space explored constitutes a thermodynamic signature of that particular Ab. Combining site plasticity may facilitate overlaps in such windows, with independent Abs converging onto common determinants with near identical binding affinities. In addition to providing for an amplified recognition potential, this networking of individual spectra of Ag specificities simultaneously facilitates the rapid recognition of Ag. Importantly, it also ensures that the primary response is composed of Abs with a high degree of "evolvability."
Multipole-accelerated capacitance extraction algorithms for 3-D structures with multiple dielectrics (1992)

The authors describe how to extend the multiple-accelerated boundary-element method for 3-D capacitance computation to the case where conductors are embedded in an arbitrary piecewise-constant dielectric medium. Results are presented to demonstrate that the method is accurate, has nearly linear computational growth, and can be nearly two orders of magnitude faster than the standard boundary-element method based on matrix factorization.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
An Ophthalmic Anesthesia Training System Using Integrated Capacitive and Hall Effect Sensors (2014)

Regional anesthesia delivery for ocular surgery involves insertion of a syringe needle into the orbital space at the proper position and orientation such that ocular structures remain undamaged while avoiding adverse systemic reactions. Additionally, anesthetic fluid must be injected at an appropriate rate to achieve painless and rapid akinesia. Training on human subjects is risky and animal cadavers do not emulate human ocular anatomy. Thus, a training system, which closely replicates human ocular anatomical structure and provides real-time qualitative feedback on the effectiveness and safety of the anesthetic procedure would significantly mitigate risks associated with real life procedures. This paper presents a rapid prototyped, anatomically precise training manikin that detects the proximity and touch of syringe needle to the extraocular muscles and alarms the trainee to avoid injury. The proximity of the needle to the muscle structure is detected using capacitive sensors integrated in the manikin. A Hall-effect sensor-based measurement scheme for detection of rate of injection from a syringe has been developed. The specially designed syringe piston provides illusion of fluid flow inside the manikin for the trainee while reducing anesthesia wastage. A virtual instrument developed measures the output from capacitive sensing electrodes and Hall-effect sensor and displays it to the trainee through a graphical user interface. The proposed capacitive touch and proximity detection schemes have been validated by tests performed on the prototype system. The rate of injection was measured in real time on a prototype syringe, demonstrating the practical use of the system for medical training purposes.
Human T and B Lymphocytes Express a Structurally Conserved Focal Adhesion Kinase, pp125 FAK (1993)

Clustering of beta 1-integrins on adherent cells with antibodies or ligands results in increased tyrosine phosphorylation and activation of a novel focal adhesion tyrosine kinase, pp125FAK. The genes encoding pp125FAK have been cloned previously from both chicken and mouse cDNA libraries, and the deduced amino acid sequences are nearly identical (94%). Two synthetic peptides derived from sequences at the carboxyl terminus of chicken pp125FAK were conjugated to ovalbumin to generate rabbit heteroantisera. Human pp125FAK was immunodetected in both T and B lymphocytes with these antisera. A basal state of pp125FAK tyrosine phosphorylation was observed in T and B lymphocytes, and its expression level was in general augmented among human T- and B-cell leukemia/lymphoma lines. Additionally, the full-length sequence of human T-cell pp125FAK (huT-FAK) was derived from a Jurkat T-cell cDNA library. huT-FAK is structurally identical with both mouse and chicken FAK, and shares 95% amino acid identity with chicken pp125FAK and has 97% homology with the mouse sequence. This high degree of evolutionary conservation between species suggests that pp125FAK is likely to have a crucial function in the cell. Expression of the full-length huT-FAK gene in COS cells showed an immunologically indistinct human pp125FAK protein compared with the endogenous primate pp125FAK. Taken together, the data indicate that this structurally conserved human T-cell pp125FAK likely functions in T- and B-cell lineages, and its altered expression in human lymphocyte tumor cell lines may contribute to their transformed phenotype.
노인의 급성 혼돈에 관한 고찰 (2004)

Purpose: The purpose of this study is to review acute confusion of aged. Method: Review article. Rusult: Acute confusion is a common problem seen by...
Myofibroblast Differentiation by PTEN (2006)

Peer Reviewed
Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference (2015)

The discovery that the machinery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 bacterial immune system can be re-purposed to easily create deletions, insertions and replacements in the mammalian genome has revolutionized the field of genome engineering and re-invigorated the field of gene therapy. Many parallels have been drawn between the newly discovered CRISPR-Cas9 system and the RNA interference (RNAi) pathway in terms of their utility for understanding and interrogating gene function in mammalian cells. Given this similarity, the CRISPR-Cas9 field stands to benefit immensely from lessons learned during the development of RNAi technology. We examine how the history of RNAi can inform today's challenges in CRISPR-Cas9 genome engineering such as efficiency, specificity, high-throughput screening and delivery for in vivo and therapeutic applications.
Les enjeux des transformations de la recherche (2006)

La recherche fait aujourd'hui l'objet de reformes importantes dont un objectif majeur est de redefinir les relations entre la recherche publique et le monde economique. En France, l'organisation de la recherche est, dans cette perspective, durement critiquee : trop coupee du monde economique, elle encouragerait des travaux depourvus d'utilite economique immediate. Les AA. de cet article comparent la situation aux Etats Unis, ou les politiques d'inspiration neoliberale ont ete mises en oeuvre des 1980, mais leurs effets donnent aujourd'hui lieu a un debat qui risque de se developper en Europe d'ici peu
Retinal vein occlusion. (1992)

Retinal vein occlusion is a common form of retinal vascular disease, especially in middle-aged and older individuals. The diagnosis is based on the funduscopic finding of retinal vein dilatation in association with retinal hemorrhages and cotton-wool spots. The pathology can involve the entire venous system or can be limited to a branch of the central retinal vein. Retinal vein occlusion can be distinguished clinically from diabetic retinopathy and other retinal diseases. Treatment for the acute phase of retinal vein occlusion has been disappointing. However, some late complications, such as persistent macular edema and neovascularization of the iris and retina, respond well to retinal photocoagulation. The family physician has an important role in detecting and controlling risk factors for retinal vein occlusion, including hypertension, diabetes mellitus and hyperviscosity syndromes.
Amniotic membrane grafts, "fresh" or frozen? A clinical and in vitro comparison (2001)

Although both procedures led to an improvement in visual acuity, "fresh" tissue performed no better than frozen in promoting re-epithelialisation. The authors suggest that logistical, safety, and cost considerations outweigh any benefits of using "fresh" as opposed to frozen graft material.
Effects of Long-Term Cholecalciferol Supplementation on Mineral Metabolism and Calciotropic Hormones in Chronic Kidney Disease (2008)

Vitamin D insufficiency/deficiency in CKD significantly improved after the 12-month cholecalciferol treatment, with higher dose being more effective and equally safe. Further studies of vitamin D(3) effects on bone metabolism are warranted.
-Mannosidosis:Functional Cloning of the Lysosomal -Mannosidase cDNA and Identification of a Mutation in Two Affected Siblings (1997)

a-Mannosidosis (MIM 248500) is an autosomal recessive lysosomal storage disorder resulting from deficient activity of lysosomal alpha-mannosidase (LAMAN) (EC 3.2.1.24). The disease is characterized by massive intracellular accumulation of mannose-rich oligosaccharides with resulting mental retardation, hearing loss, immune deficiency and skeletal changes. We report here the purification and characterization of human placenta LAMAN. The enzyme is synthesized as a single-chain precursor which is processed into three glycopeptides of 70, 42 and 15 kDa. The 70 kDa peptide is further partially proteolysed into three more peptides that are joined by disulfide bridges. The laman cDNA sequence was assembled from overlapping fragments obtained by PCR on human fibroblast and human lung cDNA. The deduced amino acid sequence contains a putative signal peptide of 48 amino acids followed by a polypeptide sequence of 962 amino acids. Northern blot analyses revealed a single transcript of approximately 3.5 kb present in all tissues examined but at varying levels. Two affected siblings of Palestinian origin were homozygous for a mutation that causes a His-->Leu replacement at a position which is conserved among class 2 alpha-mannosidases from several species.
Maltose Transport in Escherichia coli K12. A Comparison of Transport Kinetics in Wild-Type and lamba-Resistant Mutants with the Dissociation Constants of the Maltose-Binding Protein as Measured by Fluorescence Quenching (1976)

The kinetic parameters for the maltose transport system in Escherichia coli K12 were determined with maltose and maltotriose as substrates. The system exhibits an apparent Km of 1 muM for maltose and 2 muM for maltotriose. The V of entry was determined as 2.0 and 1.1 nmol substrate/min per 10(8) cells. Mutations in lamB, the structural gene for the receptor protein of phage lambda, increased the Km for maltose transport by a factor of 100-500 without influencing the maximal rate of transport. Maltotriose is no longer transported in these lamB mutants. The maltose-binding protein, an essential component of the maltose transport system, was found to exhibit substrate-dependent fluorescence quenching. This phenomenon was used to determine dissociation constants and to estimate the rate of ligand dissociation. A Kd of 1 muM for maltose and of 0.16 muM for maltotroise was found. From the comparison of the kinetic parameters of transport of maltose and maltotriose in wild-type and lambda-resistant mutants with the binding constants for both sugars to purified maltose-binding protein, we conclude that the lambda receptor facilitates the diffusion of maltose and maltodextrins through the outer membrane.
Highly Conductive and Stretchable Silver Nanowire Conductors (2012)

A highly conductive and stretchable conductor with silver nanowires (AgNWs) embedded just below the surface of poly(dimethylsiloxane) (PDMS) is fabricated. Stable conductivity of 5285 S cm−1 is achieved in a large range of tensile strain (0–50%) after a few cycles of stretching/releasing of the substrate. This stable electric response is due to buckling of the AgNW/PDMS thin layer, which is attributed to irreversible sliding of the AgNWs in the PDMS matrix. AgNWs can be printed to fabricate patterned stretchable conductors with feature size as small as 50 μm.
A generalized entropy criterion for Nevanlinna-Pick interpolation with degree constraint (2001)

We present a generalized entropy criterion for solving the rational Nevanlinna-Pick problem for n+1 interpolating conditions and the degree of interpolants bounded by n. The primal problem of maximizing this entropy gain has a very well-behaved dual problem. This dual is a convex optimization problem in a finite-dimensional space and gives rise to an algorithm for finding all interpolants which are positive real and rational of degree at most n. The criterion requires a selection of a monic Schur polynomial of degree n. It follows that this class of monic polynomials completely parameterizes all such rational interpolants, and it therefore provides a set of design parameters for specifying such interpolants. The algorithm is implemented in a state-space form and applied to several illustrative problems in systems and control, namely sensitivity minimization, maximal power transfer and spectral estimation.
Coexistence of papillary thyroid cancer with Hashimoto thyroiditis (2012)

HT was associated with a threefold increase of PTC prevalence as compared to other non-HT thyroid diseases, and the spread of PTC to level VI lymph nodes was four times more frequent in HT than in non-HT patients.
Use of a bacterial expression vector to identify the gene encoding a major core protein of vaccinia virus (1985)

The DNA sequence of a vaccinia virus late gene contains an open reading frame that corresponds to the 28,000-dalton (28K) polypeptide made by in vitro translation of hybrid-selected mRNA. To further characterize the protein product of this late gene, we cloned a segment of DNA containing part of the open reading frame into a bacterial expression vector. The fusion protein produced from this vector, containing 151 amino acids of the predicted vaccinia virus protein, was used to immunize rabbits. The resulting antiserum specifically bound to a major 25K structural protein that is localized in the core of vaccinia virions, as well as to a 28K protein found in infected cells. Pulse-chase experiments indicated that the 25K core protein is originally made as a 28K precursor.
Positional distribution of highly unsaturated fatty acids in triacyl‐sn‐glycerols of Artemia nauplii enriched with docosahexaenoic acid ethyl ester (2001)

This paper presents the positional distribution of fatty acids in triacyl-sn-glycerols (TAG) of Artemia nauplii used in aquaculture as a live food for marine fish larvae. The nauplii were enriched with docosahexaenoic acid (DHA) ethyl ester (EE) in the form of gelatin-acacia microcapsules for 4, 18, and 24 h. TAG of the initial, enriched, and unenriched Artemia nauplii were subjected to stereospecific analysis. A remarkable increase of DHA content in the enriched Artemia TAG confirmed the view that DHA-EE is effectively assimilated and incorporated into the TAG fraction of Artemia nauplii. TAG of the nauplii enriched with 25 mg/L of DHA-EE contained DHA at concentrations of 5.9-6.8, 4.3-6.0, and 14.3-22.3 mol% in the sn-1, sn-2, and sn-3 positions, respectively. When the nauplii were enriched with 100 mg/L of DHA-EE, proportions of DHA in the sn-1, sn-2, and sn-3 positions were 5.2-8.6, 3.9-6.0, and 12.2-25.4 mol%, respectively. In all of the enriched Artemia, DHA was preferentially located in the sn-3 position followed in sequence by the sn-1 and sn-2 positions. The lower content of DHA in the sn-1 and sn-2 positions was consistent with low content of this acid in 1,2-diacyl-sn-glycerophospholipids. When fish larvae are reared on Artemia nauplii enriched with EE-type DHA oil, the larvae feed on DHA esterified in TAG with a positional distribution pattern similar to that of marine mammals (sn-3 >> sn-1 > sn-2) rather than that of fish or marine invertebrates (sn-2 >> sn-3 > sn-1).
Biomarkers of Inflammatory Bowel Disease (2014)

Inflammatory bowel disease (IBD) is a chronic disease mostly involved with intestine with unknown etiology. Diagnosis, evaluation of severity, and prognosis are still present as challenges for physicians. An ideal biomarker with the characters such as simple, easy to perform, noninvasive or microinvasive, cheap, rapid, and reproducible is helpful for patients and clinicians. Currently biomarkers applied in clinic include CRP, ESR, pANCA, ASCA, and fecal calprotectin. However, they are far from ideal. Lots of studies are focused on seeking for ideal biomarker for IBD. Herein, the paper reviewed recent researches on biomarkers of IBD to get advances of biomarkers in inflammatory bowel disease.
Developing Lipid Nanoparticle-Based siRNA Therapeutics for Hepatocellular Carcinoma Using an Integrated Approach (2013)

Successful siRNA therapeutics requires the optimal integration of multiple components, including an efficient delivery system, a disease indication that is appropriate for siRNA-based therapy, and a potent and nontoxic siRNA against a robust therapeutic target. Although all currently available delivery systems have limitations, it is important to recognize that a careful selection of the disease indication, therapeutic target, and siRNA molecule could partially compensate for deficiencies associated with the delivery system and makes it possible to advance a therapeutic siRNA regimen. In this study, we present the development of siRNA therapeutics for hepatocellular carcinoma using an integrated approach, including the development of an efficient lipid nanoparticle delivery system, the identification of a robust therapeutic target that does not trigger liver toxicity upon target knockdown, and the selection of potent and nonimmunogenic siRNA molecules against the target. The resulting siRNA-containing lipid nanoparticles produced significant antitumor efficacy in orthotopic hepatocellular carcinoma models, and, thus, represent a promising starting point for the development of siRNA therapeutics for hepatocellular carcinoma.
Ultrasound in Liquid and Solid Metals (1994)

Ultrasound in Liquid and Solid Metals focuses on the effect of intensive ultrasound on metals, including the analysis of the development of cavitation and acoustic flows in melts, mechanism of metals' spraying and crystallization, the formation of dislocation structure in crystals, diffusion, phase transformation and plastic deformation. Physical fundamentals of intensive ultrasound effects are covered, and detailed discussions are presented on the engineering principles of equipment and material design for the practical use of ultrasound in the refining of melts, crystallization of ingots and moulds, pulverization, plating, pressure working of metals and surface strengthening.