gebhart/patrepeval huggingface dataset:
Natural language, knowledge representation and discourse (1991)

The principal objective of this project is to develop a system for representing and reasoning about the discourse context in extended man-machine dialogs. Current focus areas include the development of a general theory of multi-agent planning to account for the structure of natural-language dialog, the development of a general knowledge representation for capturing a wide range of natural language semantics, and the development of a general, error-tolerant parser and semantic interpreter for English that can be guided by discourse information. Specifically, we are developing a model of discourse plans that includes actions such as introducing a new topic, as well as the actions of clarifying, correcting or acknowledging parts of the previous dialog. We are exploring how far the planning approach can be extended, and how the "traditional" language components. i.e. parsing, semantic interpretation and discourse processing, relate to the planning component.
Characterization of epitaxial layers by the depth dependence of boron diffusivity (1992)

Differences in boron diffusivity have been used to characterize epitaxially grown silicon layers. After oxidation-enhanced diffusion of boron spikes, a decrease in boron diffusivity with increasing depth is observed in epitaxial silicon layers grown by molecular beam epitaxy and fast gas switching vapor deposition, in contrast to layers grown by low-temperature chemical vapor deposition. The reduced boron diffusivity is thought to be caused by an oversaturation of vacancy defects, acting as interstitial traps, suppressing the diffusion of boron.
A road to future broadband wireless access: MIMO-OFDM-Based air interface (2005)

Orthogonal frequency-division multiplexing is a popular method for high-data-rate wireless transmission. OFDM may be combined with multiple antennas at both the access point and mobile terminal to increase diversity gain and/or enhance system capacity on a time-varying multipath fading channel, resulting in a multiple-input multiple-output OFDM system. In this article we give a brief technical overview of MIMO-OFDM system design. We focus on various research topics for the MIMO-OFDM-based air interface, including spatial channel modeling, MIMO-OFDM transceiver design, MIMO-OFDM channel estimation, space-time techniques for MIMO-OFDM, and error correction code. The corresponding link-level simulation results are encouraging, and show that MIMO-OFDM is a promising road to future broadband wireless access.
Molecular Cloning of a Human Genomic Region Containing the H Blood Group α(1,2)Fucosyltransferase Gene and Two H Locus-related DNA Restriction Fragments (1995)

We have used the human H blood group alpha(1,2)fucosyltransferase (FUT1) cDNA to screen chromosome 19 cosmid libraries in a search for the human Secretor (Se) blood group gene (FUT2). One cosmid has been isolated that contains two distinct segments that cross-hybridize with FUT1. We have assembled a 100-kilobase (kb) cosmid contig, localized to 19q13.3, encompassing FUT1 and the two FUT1-related sequences, termed Sec1 and Sec2, for Secretor candidate 1 and 2. Sec1 and Sec2 are separated by 12 kb and are 65.5 kb and 35 kb apart, respectively, from the FUT1 gene. We used a cosmid-dependent direct cDNA selection method to clone a cDNA corresponding to a transcript that emanates from Sec2. This cDNA detects a 3.35-kb transcript in human tissues known to express the Se locus. Together with sequence and expression data reported in the accompanying article (Kelly, R. J., Rouquier, S., Giorgi, D., Lennon, G. G., and Lowe, J. B. (1995) J. Biol. Chem. 270, 4640-4649), these data demonstrate that Sec2 corresponds to the human Se blood group locus (FUT2). Our results furthermore define the physical relationship between the H and Se loci and confirm a hypothesis that these two loci represent distinct but closely linked alpha(1,2)fucosyltransferase genes.
Sirolimus for Angiomyolipoma in Tuberous Sclerosis Complex or Lymphangioleiomyomatosis (2008)

Angiomyolipomas regressed somewhat during sirolimus therapy but tended to increase in volume after the therapy was stopped. Some patients with lymphangioleiomyomatosis had improvement in spirometric measurements and gas trapping that persisted after treatment. Suppression of mTOR signaling might constitute an ameliorative treatment in patients with the tuberous sclerosis complex or sporadic lymphangioleiomyomatosis. (ClinicalTrials.gov number, NCT00457808.)
Synthesis and Biological Activities of 4-Phenyl-5-pyridyl-1,3-thiazole Derivatives as p38 MAP Kinase Inhibitors (2005)

A novel series of 4-phenyl-5-pyridyl-1,3-thiazole analogues possessing potent in vitro inhibitory activity against p38 mitogen-activated protein kinase and the release of tumor necrosis factor-alpha (TNF-alpha) from human monocytic THP-1 cells stimulated by lipopolysaccharide has been identified. Subsequent structure-activity relationship (SAR) studies and optimization for absorption, distribution, metabolism, and elimination (ADME) profiles led to the identification of compounds 7 g and 10b as orally active lead candidates that block the in vivo production of proinflammatory cytokine (TNF-alpha). In pharmacokinetic studies, compound 10b showed good oral administration in mice and demonstrated significant in vivo anti-inflammatory activity in an anti-collagen monoclonal antibody-induced arthritis mouse model (minimum effective dose (MED)=30 mg/kg). Further elucidation of this class of compounds may provide novel anti-inflammatory agents, such as anti-rheumatoid arthritis drugs.
Inhibition of nuclear factor κB by prostaglandin A1: An effect associated with heat shock transcription factor activation (1997)

Prostaglandins (PGs) function as intracellular signal mediators in the regulation of a variety of physiological and pathological processes, including inflammation and immune responses. Cyclopentenone PGs are characterized by antiviral activity against several viruses, including human immunodeficiency virus type 1 (HIV-1), and by the ability to induce heat shock protein expression through activation of the heat shock transcription factor. Here we report that PGA1 is a potent inhibitor of nuclear factor-kappa B (NF-kappa B) activation in human cells and of NF-kappa B-dependent HIV-1 transcription in long terminal repeat-chloramphenicol acetyl-transferase transient transfection experiments. PGA1 acts by inhibiting phosphorylation and preventing degradation of the NF-kappa B inhibitor I kappa B-alpha. Inhibition does not require protein synthesis, is dependent on the presence of a reactive cyclopentenonic moiety, and is associated with heat shock transcription factor activation. Because NF-kappa B is critically involved in the activation of immunoregulatory and viral genes, inhibition of its activity could be a major component of the immunosuppressive and antiviral activity of PGs.
Effect of Ferric Carboxymaltose on Exercise Capacity in Patients With Chronic Heart Failure and Iron Deficiency (2017)

URL: http://www.clinicaltrials.gov. Unique identifier: NCT01394562.
ERα down-regulation plays a key role in silibinin-induced autophagy and apoptosis in human breast cancer MCF-7 cells (2015)

The estrogen receptor alpha (ERα) has been proven to be one of the most important therapeutic targets in breast cancer over the last 30 years. Previous studies pointed out that a natural flavonoid, silibinin, induced apoptosis in human breast cancer MCF-7 cells. In the present study we report that exposure of MCF-7 cells to silibinin led to cell death through the down-regulation of ERα expression. Silibinin-induced apoptosis of MCF-7 cells through up-regulation of caspase 6 due to ERα signalling repression was further boosted by ERα antagonist. Moreover, up-regulation of autophagy induced by silibinin accounted for apoptotic exacerbation, being further enhanced by ERα inhibition. Upon ERα activation, series of downstream signalling pathways can be activated. We found that silibinin reduced the expressions of Akt/mTOR and extracellular-signal-related kinase (ERK), which respectively accounted for the induction of autophagy and apoptosis. These effects were further augmented by co-treatment with ERα inhibitor. We conclude that the treatment with silibinin of ERα-positive MCF-7 cells down-regulates the expression of ERα, and subsequently mTOR and ERK signaling pathways, ERα downstream, finally resulting in induction of autophagy and apoptosis.
Enhanced immunogenicity of recombinant peptide fusions containing multiple copies of a heterologous T helper epitope (1990)

We have examined the immune response against the nonimmunogenic heat-stable enterotoxin (STa) of enterotoxigenic Escherichia coli using recombinant fusion proteins containing the STa-peptide linked to an IgG-binding analogue of protein A and varying numbers of the T helper epitope 323-339 from ovalbumin (ova). By immunization of inbred strains of mice with a series of STa fusion proteins, containing up to four copies of ova tandemly multiplied, we demonstrated that the anti-STa antibody response is controlled by ova-specific T helper cells in a genetically restricted manner. In the responding mouse strains (2 out of 3 tested), the level of antibody production was increased by addition of multiple ova epitopes, the anti-STa response being considerably higher to fusion proteins containing four than one or two ova epitopes.
Plant Protein Kinase Families and Signal Transduction (1995)

Enzymes of the eukaryotic protein kinase superfamily catalyze the reversible transfer of the gamma-phosphate from ATP to amino acid side chains of proteins. Protein kinase function can be counteracted by the action of phosphoprotein phosphatases. Phosphorylation status of a protein can have profound effects on its activity and interaction with other proteins. An estimated 1 to 3% of functional eukaryotic genes encode protein kinases, suggesting that they are involved in many aspects of cellular regulation and metabolism. In plants, protein phosphorylation has been implicated in responses to many signals, including light, pathogen invasion, hormones, temperature stress, and nutrient deprivation. Activities of several plant metabolic and regulatory enzymes are also controlled by reversible phosphorylation. As might be expected from this diversity of function, there is a large array of different protein kinases. Purification of protein kinases and their subsequent cloning, facilitated by the PCR and advances in homology-based cloning techniques, as well as functional analyses, including complementation of conditional yeast mutants and positional cloning of mutant plant genes, has already led to identification of more than 70 plant protein kinase genes. However, the precise functional roles of specific protein kinases and phosphatases during plant growth and development have been elucidated for only a few.
Lectin Interactions With the Jurkat Leukemic T-Cell Line: Quantitative Binding Studies and Interleukin-2 Production (1988)

Phytohemagglutinin (PHA), concanavalin A (Con A), pea lectin, and wheat germ agglutinin (WGA) have been used to investigate their binding properties to Jurkat 77 6.8 leukemic human T cells and their ability to induce these cells to produce interleukin-2 (IL-2). Binding studies showed that the Jurkat cells fixed 0.82 +/- 0.11 microgram pea lectin, 2.02 +/- 0.17 micrograms Con A, 1.85 +/- 0.07 micrograms PHA and 8.88 +/- 0.61 micrograms WGA. Scatchard plots were linear, indicating that the binding process was homogeneous with respect to the binding constant. PHA and Con A bound with the highest affinity [Kass (apparent) approximately equal to 9 x 10(9) M-1], followed by pea lectin and WGA [Kass (apparent) approximately equal to 3 x 10(9) M-1]. The number of lectin binding sites was in agreement with the results of saturation experiments. We also evaluated the effect of the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the binding process. Results show that there were no gross alterations in the value of (apparent) Kass in the case of PHA and WGA. In contrast, the presence of TPA decreased the affinity of Con A and modified the Scatchard profile for pea lectin, which was curvilinear with a concavity turned upward. In this case, data were (apparent) K1 = 17.7 x 10(9) M-1 ("high-affinity" sites) and (apparent) K2 = 2.6 x 10(9) M-1 ("low-affinity" sites). The four lectins shared the ability to stimulate Jurkat 77 6.8 cells to secrete IL-2. Optimal lectin concentrations were 20 micrograms/ml (PHA) and 50 micrograms/ml (WGA and Con A). Pea lectin failed to display a dose-response relationship, and IL-2 production increased proportionally with lectin concentration. Con A was the most efficient stimulator (250 U/ml), followed by WGA (160 U/ml) and PHA (108 U/ml). Addition of TPA potentiated Jurkat cell response. In this case, Con A and pea lectin were the most efficient stimulators (470 U/ml), followed by PHA (316 U/ml) and WGA (271 U/ml). Here, also, pea lectin failed to show a dose-response relationship. Our data do not reveal an obvious relationship between the binding parameters and the ability of the lectins to induce IL-2 production. We suggest that the efficiency of the four lectins to stimulate IL-2 production in the case of the Jurkat 77 6.8 variant is most likely related to the nature of their specific cell surface receptor(s).
Convolution engine (2013)

This paper focuses on the trade-off between flexibility and efficiency in specialized computing. We observe that specialized units achieve most of their efficiency gains by tuning data storage and compute structures and their connectivity to the data-flow and data-locality patterns in the kernels. Hence, by identifying key data-flow patterns used in a domain, we can create efficient engines that can be programmed and reused across a wide range of applications. We present an example, the Convolution Engine (CE), specialized for the convolution-like data-flow that is common in computational photography, image processing, and video processing applications. CE achieves energy efficiency by capturing data reuse patterns, eliminating data transfer overheads, and enabling a large number of operations per memory access. We quantify the tradeoffs in efficiency and flexibility and demonstrate that CE is within a factor of 2-3x of the energy and area efficiency of custom units optimized for a single kernel. CE improves energy and area efficiency by 8-15x over a SIMD engine for most applications.
In Vitro Methods in Developmental Toxicology : Use in Defining Mechanisms and Risk Parameters (1990)

Introduction. In Vitro Evaluations of Human Placental Functions and Toxic Responses. Pharmacokinetic Aspects of In Vitro Teratogenicity Studies: Comparison to In Vivo. Application of Cell-Cell Communication Assays to Problems of Developmental Toxicology. Cell Migration and Contractility. Analysis of Cell Proliferation in Developing Orofacial Tissue. Membrane Lipid Metabolism and Development of the Palate. In Vitro Assays of Cell Adhesion: Application to Teratogenicity Testing. In Vitro Approaches to the Study of Embryonic Cell Death in Developmental Toxicity. The Regulation of Genes Encoding Extracellular Matrix Proteins: In Vitro Studies and Relationships to Teratogenic Mechanisms. In Vitro Assays in Developmental Toxicology: Their Potential Application in Risk Assessment.
Creating biomimetic micro-environments with synthetic polymer-peptide hybrid molecules (1998)

In designing polymers that can act as tissue engineering templates it is beneficial to consider methods of mimicking the natural support structures used by the human body to guide the behavior and development of cells within tissues. The well-known RGD cell adhesion ligand provides a simple mechanism of creating polymer surfaces that mimic the extracellular matrix. This paper considers the methods that have been used to attach such motifs to synthetic polymers. In general there are two strategies: the formation of polymer-peptide hybrid molecules, or the immobilization of the ligand on the fabricated surface of the polymer. The three major synthetic strategies of creating polymer-peptide hybrids are reviewed.
Percutaneous endovascular embolization of intracerebral arteriovenous malformations. Experience in 72 cases. (1995)

Endovascular therapeutic embolization of arteriovenous malformations (AVMs) of the brain was performed in 72 patients between October 1986 and March 1993. From October 1986 to May 1991, 38 patients in this series were treated with isobuty1-2-cyanoacrylate (IBCA) glue. After June 1991, in the remaining 34 patients the embolic materials used included surgical silk (5-0), polyvinyl alcohol (PVA), ethanol, and estrogen. After treatment, 29 patients (40.3%) had complete angiographic obliteration of AVM, 15 (21%) transient neurologic postembolization deficit, and 3 (4%) permanent deficit without death. Embolic agents and procedures are discussed as to their curative effects, safety, normal perfusion pressure breakthrough (NPPB) problem, with silk mixture fluid being considered preferable.
Subambient Pressure Ionization with Nanoelectrospray Source and Interface for Improved Sensitivity in Mass Spectrometry (2008)

A nanoelectrospray ionization mass spectrometry (ESI-MS) source and interface has been designed that enables efficient ion production and transmission in a 30 Torr pressure environment using solvents compatible with typical reversed-phase liquid chromatography (RPLC) separations. In this design, the electrospray emitter is located inside the mass spectrometer in the same region as an electrodynamic ion funnel. This avoids the use of a conductance limiting ion inlet, as required by a conventional atmospheric pressure ESI source, and allows more efficient ion transmission to the mass analyzer. The new subambient pressure ionization with nanoelectrospray (SPIN) source improves instrument sensitivity and enables new electrospray interface designs, including the use of multi-emitter approaches. Performance of the SPIN source was evaluated by electrospraying standard solutions at 300 nL/min and comparing results with those obtained from a standard atmospheric pressure ESI source that used a heated capillary inlet. This initial study demonstrated an approximately 5-fold improvement in sensitivity when the SPIN source was used compared to a standard atmospheric pressure ESI source. The importance of desolvation was also investigated by electrospraying at different flow rates, which showed that the ion funnel provided an effective desolvation region to aid the creation of gas-phase analyte ions.
Unidirectional long short-term memory recurrent neural network with recurrent output layer for low-latency speech synthesis (2015)

Long short-term memory recurrent neural networks (LSTM-RNNs) have been applied to various speech applications including acoustic modeling for statistical parametric speech synthesis. One of the concerns for applying them to text-to-speech applications is its effect on latency. To address this concern, this paper proposes a low-latency, streaming speech synthesis architecture using unidirectional LSTM-RNNs with a recurrent output layer. The use of unidirectional RNN architecture allows frame-synchronous streaming inference of output acoustic features given input linguistic features. The recurrent output layer further encourages smooth transition between acoustic features at consecutive frames. Experimental results in subjective listening tests show that the proposed architecture can synthesize natural sounding speech without requiring utterance-level batch processing.
Genetic engineering of crop plants: from genome to gene (1997)

Genetic engineering of crop plants has been in progress since the dawn of agriculture, about 10 000 years ago. For millennia the genetic make-up of our crop plants has been changed by mankind's selection of naturally occurring variants. As the trade routes were developed, novel plant types were introduced into new environments and provided more variation from which to choose. At the end of the nineteenth century an understanding of the laws of heredity was gained and plant breeding protocols were devised whereby selection became accompanied by deliberate crossing. As the knowledge of the genetic structure of crop plants improved, new ways of manipulation were invented and exploited. Indeed plant breeding became a testing bed for new ideas in genetics. For the plant breeder the techniques which were most widely employed in the past were those which aided breeding, for example techniques which speeded up the production of new varieties, but still used traditional routes of crossing and selection. This was a transitional phase between plant breeding as an art and plant breeding as a science.
Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae (1996)

The nifLA operon of Klebsiella pneumoniae codes for the two antagonistic regulatory proteins which control expression of all other nitrogen fixation genes. NifA is a transcriptional activator, and NifL inhibits NifA. The importance of a correct NifL-NifA stoichiometry for efficient regulation of nitrogen fixation genes has been investigated by constructing a strain with an altered nifL-nifA gene dosage ratio, resulting from the integration of an extra copy of nifA. Results showed that a balanced synthesis of both gene products is essential for correct regulation. Effects of mutations provoking translation termination of nifL upstream or downstream of its natural stop codon, combined with overproduction of both proteins when the genes are transcribed and translated from signals of the phi10 gene of the phage T7, showed that, in addition to the previously reported transcriptional polarity, there is translational coupling between nifL and nifA. In spite of the apparently efficient ribosome binding site of nifA, its rate of independent translation is very low. This is due to a secondary structure masking the Shine-Dalgarno sequence of nifA, which could be melted by ribosomes translating nifL. Mutational analysis confirmed the functional significance of the secondary structure in preventing independent translation of nifA. Translational coupling between the two cistrons is proposed as an efficient mechanism to prevent production of an excess of NifA, which would affect the normal regulation of nitrogen fixation genes.
Secondary prophylaxis with factor IX concentrates: continuous infusion. (2008)

Haemophilia patients may require prolonged treatment during peri-operative period or life-threatening bleedings. Intermittent bolus infusions of factor concentrates have been used successfully for many years. However, pharmacokinetics may vary among products and patients, and the wide fluctuations in factor levels during therapy can make management inaccurate and unsafe. Specific protocols for continuous infusion have been developed, which may decrease factor utilization, facilitate laboratory monitoring of factor levels, and may decrease the overall cost of therapy. Continuous infusion of different clotting factor concentrates (factor VIII, factor IX, porcine factor VIII, rFVIIa, and activated prothrombin complex concentrates) has been associated with excellent haemostasis and safety: indeed, by continuous infusion unsafe low troughs or dangerous high concentrations can be avoided. In this review, the pharmacokinetic aspects of the continuous infusion of plasma-derived and recombinant factor IX concentrates are showed. Finally, the advantages and disadvantages of continuous infusion are discussed.