gebhart/patrepeval huggingface dataset:
Roles of cardiac norepinephrine uptake 1 and 2 in evaluation of LMI1195, a new cardiac PET neuronal imaging agent, in rats, rabbits and nonhuman primates (2011)

1099 Objectives Norepinephrine (NE) released in the heart is mainly cleared by neuronal uptake 1 (NE transporter) in rabbits, nonhuman primates (NHP) and humans, and by uptake 1 and 2 in rats. LMI1195, like NE and 123I- meta-iodobenzylguanidine (MIBG), is a substrate for uptake 1 and labeled with 18F. This study examined species differences associated with cardiac uptake 1 and 2 for LMI1195 heart uptake. Methods Desipramine (mg/kg), a selective uptake 1 inhibitor, was used to block cardiac uptake 1 in rats (10, ip), rabbits (1, iv) and NHPs (0.5, iv). 6-hydroxydopamine (mg/kg), a neurotoxin, was injected to induce sympathetic denervation in rats (100, ip) and rabbits (25, iv for 4 days). LMI1195 or MIBG heart uptake was assessed by tissue sampling at 60 minutes post injection. LMI1195 imaging was also performed. Results In rats, uptake 1 blockade did not alter LMI1195 heart uptake compared to the control [1.41±0.07 vs. 1.47±0.22 % injected dose per gram tissue (%ID/g)]. In contrast, LMI1195 heart uptake was reduced by 68% in uptake 1 blocked rabbits. In sympathetic denervated rats, LMI1195 heart uptake was similar to the control (2.18±0.39 vs. 2.58±0.76 %ID/g). However, the uptake decreased markedly (79%) in denervated rabbits. Similar results were found in MIBG heart uptake in rats and rabbits with uptake 1 blockade or sympathetic denervation. Consistently, LMI1195 cardiac imaging showed comparable myocardial activity in sympathetic denervated rats to the control, but marked activity reduction in denervated rabbits and uptake 1 blocked rabbits and NHPs. Conclusions In rabbits and NHPs with uptake 1 as the main cardiac NE transporter, similar to humans, LMI1195 shows high selectivity to neuronal uptake 1 and can be used in evaluation of cardiac sympathetic denervation. However, due to the high cardiac uptake 2 expression, evaluation of uptake 1 substrate based neuronal imaging agents in rats should be used cautiously
Core 3 synthase is down-regulated in colon carcinoma and profoundly suppresses the metastatic potential of carcinoma cells (2005)

The core 3 structure of the O-glycan, GlcNAcbeta1-3GalNAcalpha1-Ser/Thr, an important precursor in the biosynthesis of mucin-type glycoproteins, is synthesized by beta1,3-N-acetylglucosaminyltransferase 6 (beta3Gn-T6; core 3 synthase). We generated an anti-beta3Gn-T6 mAb (G8-144 mAb) and performed immunohistochemical analyses. In normal stomach and colon, beta3Gn-T6 was strongly expressed in the Golgi region of epithelia. In contrast, its expression was markedly down-regulated in gastric and colorectal carcinomas. Tissue specimens from a familial adenomatous polyposis patient showed a clear correlation between the down-regulation of beta3Gn-T6 expression and the degree of dysplasia/neoplasia. In vitro, the level of beta3Gn-T6 transcript was increased according to the differentiation of Caco-2 cells. These results suggested that the expression of beta3Gn-T6 is closely regulated during differentiation and dedifferentiation. beta3Gn-T6 would be a useful marker for distinguishing between benign adenomas and premalignant lesions. HT1080 FP-10 cells stably transfected with the beta3Gn-T6 gene showed a decrease in the core 1 structure, Galbeta1,3GalNAcalpha1-Ser/Thr, probably due to competition between the core 1 synthase and core 3 synthase. The migration activity of the transfectants was markedly lower than that of mock transfectants in vitro, and lung metastasis after i.v. injection of the transfectants into nude mice was significantly suppressed. These findings indicated that the core structures of O-glycans are profoundly involved in the metastatic capacity of cancer cells.
Distribution of Genes Encoding Nucleoid-Associated Protein Homologs in Plasmids (2011)

Bacterial nucleoid-associated proteins (NAPs) form nucleoprotein complexes and influence the expression of genes. Recent studies have shown that some plasmids carry genes encoding NAP homologs, which play important roles in transcriptional regulation networks between plasmids and host chromosomes. In this study, we determined the distributions of the well-known NAPs Fis, H-NS, HU, IHF, and Lrp and the newly found NAPs MvaT and NdpA among the whole-sequenced 1382 plasmids found in Gram-negative bacteria. Comparisons between NAP distributions and plasmid features (size, G+C content, and putative transferability) were also performed. We found that larger plasmids frequently have NAP gene homologs. Plasmids with H-NS gene homologs had less G+C content. It should be noted that plasmids with the NAP gene homolog also carried the relaxase gene involved in the conjugative transfer of plasmids more frequently than did those without the NAP gene homolog, implying that plasmid-encoded NAP homologs positively contribute to transmissible plasmids.
The Comorbidity of Major Depression and Anxiety Disorders (2001)

BACKGROUND: Depressive and anxiety disorders commonly occur together in patients presenting in the primary care setting. Although recognition of individual depressive and anxiety disorders has increased substantially in the past decade, recognition of comorbidity still lags. The current report reviews the epidemiology, clinical implications, and management of comorbidity in the primary care setting. METHOD: Literature was reviewed by 2 methods: (1) a MEDLINE search (1980-2001) using the key words depression, depressivedisorders, and anxietydisorders; comorbidity was also searched with individual anxiety diagnoses; and (2) direct search of psychiatry, primary care, and internal medicine journals over the past 5 years. RESULTS: Between 10% and 20% of adults in any given 12-month period will visit their primary care physician during an anxiety or depressive disorder episode (although typically for a nonpsychiatric complaint); more than 50% of these patients suffer from a comorbid second depressive or anxiety disorder. The presence of depressive/anxiety comorbidity substantially increases medical utilization and is associated with greater chronicity, slower recovery, increased rates of recurrence, and greater psychosocial disability. Typically, long-term treatment is indicated, although far less research is available to guide treatment decisions. Selective serotonin reuptake inhibitor antidepressants are the preferred treatment based on efficacy, safety, and tolerability criteria. Knowledge of their differential clinical and pharmacokinetic profiles can assist in optimizing treatment. CONCLUSION: Increased recognition of the high prevalence and negative psychosocial impact of depression and anxiety disorder comorbidity will lead to more effective treatment. While it is hoped that early and effective intervention will yield long-term benefits, research is needed to confirm this.
RNA Interference in the Nucleus (2008)

Studies of a protein that shuttles small regulatory RNA molecules into the nucleus suggest that additional factors may be required for nuclear import.
Pd Nanoparticles and Thin Films for Room Temperature Hydrogen Sensor (2009)

We report the application of palladium nanoparticles and thin films for hydrogen sensor. Electrochemically grown palladium particles with spherical shapes deposited on Si substrate and sputter deposited Pd thin films were used to detect hydrogen at room temperature. Grain size dependence of H(2) sensing behavior has been discussed for both types of Pd films. The electrochemically grown Pd nanoparticles were observed to show better hydrogen sensing response than the sputtered palladium thin films. The demonstration of size dependent room temperature H(2) sensing paves the ways to fabricate the room temperature metallic and metal-metal oxide semiconductor sensor by tuning the size of metal catalyst in mixed systems. H(2) sensing by the Pd nanostructures is attributed to the chemical and electronic sensitization mechanisms.
Germicidal Power of Sodium Hypochlorite - Effect of Addition of Alkali (1934)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGermicidal Power of Sodium Hypochlorite - Effect of Addition of AlkaliC. K. JohnsCite this: Ind. Eng. Chem. 1934, 26, 7, 787–788Publication Date (Print):July 1, 1934Publication History Published online1 May 2002Published inissue 1 July 1934https://pubs.acs.org/doi/10.1021/ie50295a024https://doi.org/10.1021/ie50295a024research-articleACS PublicationsRequest reuse permissionsArticle Views48Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Construction of a Highly Symmetric Nanosphere via a One-Pot Reaction of a Tristerpyridine Ligand with Ru(II) (2014)

A three-dimensional, highly symmetric, terpyridine-based, spherical complex was synthesized via the coordination of four novel, trisdentate ligands and six Ru(2+) ions, and it exhibits excellent stability over a wide range of pH values (1-14). Structural confirmation was obtained by NMR and ESI-TWIM-MS.
Biphasic expression of stromal cell-derived factor-1 during human wound healing (2007)

Our data suggest that SDF-1/CXCL12 is tightly regulated during wound repair. Increased expression at the wound margin may contribute to the accumulation of endothelial progenitor cells, thus accelerating neovascularization.
COMPARTMENTATION OF PROTEINS IN THE ENDOMEMBRANE SYSTEM OF PLANT CELLS (1996)

This review focuses on four interrelated processes in the plant endomembrane system: compartmentation of proteins in subdomains of the endoplasmic reticulum, mechanisms that determine whether storage proteins are retained within the ER lumen or transported out, the origin and function of biochemically distinct vacuoles or prevacuolar organelles, and the cellular processes by which proteins are sorted to vacuolar compartments. We postulate that ER-localized protein bodies are formed by a series of orderly events of protein synthesis, protein concentration, and protein assembly in subdomains of the ER. Protein concentration, which facilitates protein-to-protein interactions and subsequent protein assembly, may be achieved by the interactions with chaperones and by the localization of storage protein mRNAs. We also describe recent developments on the coexistence of two biochemically distinguishable vacuolar compartments, the possible direct role of the ER in vacuole biogenesis, and proposed mechanisms for transport of proteins from the ER or Golgi apparatus to the vacuole.
Dapsone in rosacea fulminans (2001)

Rosacea fulminans is a rare disease with female predominance characterized by abrupt onset of pustules, papules, and confluent nodules on the face. The conventional treatment consists of systemic glucocorticoids and isotretinoin. We present the case of a 56-year-old woman with a marked facial papulopustular eruption that had followed an initial period of severe seborrhoea. Conventional treatment produced no clear improvement. Dapsone treatment achieved complete healing in 5 weeks.
Transcriptome Analysis on the Inflammatory Cell Infiltration of Nonalcoholic Steatohepatitis in Bama Minipigs Induced by a Long-Term High-Fat, High-Sucrose Diet (2014)

Long-term adherence to a high-fat, high-calorie diet influences human health and causes obesity, metabolic syndrome and nonalcoholic steatohepatitis (NASH). Inflammation plays a key role in the development of NASH; however, the mechanism of inflammation induced by over-nutrition remains largely unknown. In this study, we fed Bama minipigs a high-fat, high-sucrose diet (HFHSD) for 23 months. The pigs exhibited characteristics of metabolic syndrome and developed steatohepatitis with greatly increased numbers of inflammatory cells, such as lymphocytes (2.27-fold, P<0.05), Kupffer cells (2.59-fold, P<0.05), eosinophils (1.42-fold, P<0.05) and neutrophils (2.77-fold, P<0.05). High-throughput RNA sequencing (RNA-seq) was performed to explore the systemic transcriptome of the pig liver during inflammation. Approximately 18.2 gigabases of raw sequence data were generated, and over 303 million high-quality reads were assembled into 21,126 unigenes. RNA-seq data analysis showed that 822 genes were differentially expressed in liver (P<0.05) between the HFHSD and control groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the process of inflammation involved the inflammatory signal transduction-related toll-like receptor, MAPK, and PPAR signaling pathways; the cytokine-related chemokine signaling, cytokine-cytokine receptor interaction, and IL2, IL4, IL6, and IL12 signaling pathways; the leukocyte receptor signaling-related T cell, B cell, and natural killer cell signaling pathways; inflammatory cell migration and invasion- related pathways; and other pathways. Moreover, we identified several differentially expressed inflammation-related genes between the two groups, including FOS, JUN, TLR7, MYC, PIK3CD, VAV3, IL2RB and IL4R, that could be potential targets for further investigation. Our study suggested that long-term HFHSD induced obesity and liver inflammation, providing basic insight into the molecular mechanism of this condition and laying the groundwork for further studies on obesity and steatohepatitis.
Single cell detection with micromechanical oscillators (2001)

The ability to detect small amounts of materials, especially pathogenic bacteria, is important for medical diagnostics and for monitoring the food supply. Engineered micro- and nanomechanical systems can serve as multifunctional, highly sensitive, immunospecific biological detectors. We present a resonant frequency-based mass sensor, comprised of low-stress silicon nitride cantilever beams for the detection of Escherichia coli (E. coli)-cell-antibody binding events with detection sensitivity down to a single cell. The binding events involved the interaction between anti-E. coli O157:H7 antibodies immobilized on a cantilever beam and the O157 antigen present on the surface of pathogenic E. coli O157:H7. Additional mass loading from the specific binding of the E. coli cells was detected by measuring a resonant frequency shift of the micromechanical oscillator. In air, where considerable damping occurs, our device mass sensitivities for a 15 μm and 25 μm long beam were 1.1 Hz/fg and 7.1 Hz/fg, respectively. In both cases, utilizing thermal and ambient noise as a driving mechanism, the sensor was highly effective in detecting immobilized anti-E. coli antibody monolayer assemblies, as well as single E. coli cells. Our results suggest that tailoring of oscillator dimensions is a feasible approach for sensitivity enhancement of resonant mass sensors.
Identification and microbial production of a terpene-based advanced biofuel (2011)

Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels.
Regulatory domains of the Gmhsp17.5-E heat shock promoter of soybean. (1989)

Promoter domains required for in vivo transcriptional expression of soybean heat shock gene Gmhsp17.5-E were identified by insertion-deletion mutagenesis with transgenic expression monitored in Agrobacterium tumefaciens-incited tumors of sunflower. Removal of the TATA-distal domain from position -1175 to position -259 had little effect on overall activity. The four regions contributing to promoter activity identified by this study all map within 244 base pairs from the start of transcription. The most distal cis-acting element of major significance was located from -244 to -179 and contains a conserved TATA-dyad motif centered at -220. Sequences from -179 to -40 comprise the TATA-proximal domain and include an AT-rich region and two sites containing heat shock consensus elements (HSEs). Deletion of the HSE centered at -93 (site 2) severely reduced transcriptional activity. Heat-inducible expression was also eliminated by internal deletion of either the TATA motif or the overlapping HSEs at site 1, indicating that each of these regions is also a major determinant of promoter activity.
Gabapentin Adjunctive Therapy in Neuropathic Pain States (1996)

Gabapentin provides analgesic activity for patients with neuropathic pain and has the advantage of a low side effect profile and drug toxicity.
Design of ultrasensitive probes for human neutrophil elastase through hybrid combinatorial substrate library profiling (2014)

The exploration of protease substrate specificity is generally restricted to naturally occurring amino acids, limiting the degree of conformational space that can be surveyed. We substantially enhanced this by incorporating 102 unnatural amino acids to explore the S1-S4 pockets of human neutrophil elastase. This approach provides hybrid natural and unnatural amino acid sequences, and thus we termed it the Hybrid Combinatorial Substrate Library. Library results were validated by the synthesis of individual tetrapeptide substrates, with the optimal substrate demonstrating more than three orders of magnitude higher catalytic efficiency than commonly used substrates of elastase. This optimal substrate was converted to an activity-based probe that demonstrated high selectivity and revealed the specific presence of active elastase during the process of neutrophil extracellular trap formation. We propose that this approach can be successfully used for any type of endopeptidase to deliver high activity and selectivity in substrates and probes.
Correlation Between Microseismicity and Reservoir Dynamics in a Tectonically Active Area of Colombia (2008)

Abstract BP operates the Cusiana volatile oil field and the Cupiagua gas condensate field in the Andean Mountains foothills province of Colombia (Fig. 1.). In 1992, a permanent seismic network of ten surface stations was installed in Cusiana and Cupiagua to obtain data for seismic hazard models necessary for the design of field infrastructure. The network is now in its sixteenth year of continuous operation. Currently, an average of 1000 microseismic events per month is recorded. The resulting seismological dataset is of high quality covering a range of seismic magnitudes down to about 1.0 on the Ritcher scale. Over time, the Cusiana-Cupiagua Seismic Network (CCSN) has been used for different purposes. During the past few years, it has become increasingly evident that the network and its data is an invaluable asset for evaluation of conditions relevant to production/injection operations within the reservoirs and adjacent areas. From the reservoir characterization and production operation standpoints, microseismic monitoring (also known as passive seismic) has had two main applications in Cusiana and Cupiagua: (i) to identify production/injection induced high transmissibility pathways and their temporal variations, and (ii) to image the orientation, extension, complexity, and temporal growth of hydraulic fractures. This paper is focused on the first of these applications: how microseismicity has been used as a surveillance tool to track movement of reservoir fluids away from the wellbore. A short description of the seismic network is provided. Then, the methodology for data interpretation is discussed. Finally, partial results are presented showing how microseismicity monitoring is being applied to: (i) assess transmissibility changes due to stress and pore pressure changes through time, (ii) identify potential reactivation of pre-existing weak planes, and (iii) calibrate numerical models to improve history matches. Analysis of the data shows a strong correlation between reservoir dynamics and production induced microseismicity in Cusiana and Cupiagua Fields with great potential as a surveillance tool for improved reservoir characterization and management.