gebhart/patrepeval huggingface dataset:
Methods for reducing non-specific antibody binding in enzyme-linked immunosorbent assays (1985)

Enzyme-linked immunosorbent assays were used to study (i) binding of rabbit antibodies (raised against litter mate liver plasma membrane fraction) to the immunizing membrane fraction, and (ii) binding of human antibodies to liver membrane fractions and to liver-specific lipoprotein (a liver membrane-derived antigen complex). When assays were conducted using the non-ionic detergent Tween 20 as blocking agent, high non-specific binding was encountered. With the low titre rabbit antisera high binding of non-immune test antibody and of second antibody (anti-rabbit IgG) to the immunogen, and also directly to the solid phase, was found. This was abolished by replacement of Tween 20 in the antibody diluent buffers by a non-reactive protein, casein proving to be a more effective blocking agent than either bovine serum albumin or gelatin. With human sera, high binding of human IgG to the solid phase was noted. This too was blocked by casein, but only when the anti-microbial agent Thimerosal was included in the casein buffer, and when Tween 20 in the wash buffer was replaced by casein-Thimerosal so that the solid phase was exposed to casein before incubation with the test serum. The casein buffers described may prove of general value in solid-phase assays where high non-specific binding is encountered.
Cerebral emboli and depressive symptoms in dementia (2006)

Spontaneous cerebral emboli are associated with clinically relevant depressive symptoms in dementia, and further research is needed to explore the nature of this relationship.
Combination Therapy of Established Tumors by Antibodies Targeting Immune Activating and Suppressing Molecules (2010)

The blockade of immune suppression against antitumor responses is a particularly attractive strategy when combined with agents that promote tumor-specific CTLs. In this study, we have attempted to further improve the CTL induction and potent antitumor efficacy of a combination mAb-based therapy (termed "trimAb therapy") that comprises tumor cell death-inducing anti-death receptor 5 mAb and immune activating anti-CD40 and anti-CD137 mAbs. Among trimAb-treated tumors, the infiltration of CD4(+) Foxp3(+) cells was greater in progressing tumors compared with stable tumors. Blockade of CTLA-4 (CD152)-mediated signals by an antagonistic mAb substantially increased the tumor rejection rate of trimAb therapy, although the immune responses of draining lymph node cells were not augmented. Interestingly, by comparison, additional treatment with agonistic anti-glucocorticoid-induced TNF receptor mAb, antagonistic anti-programmed death-1 (CD279) mAb, or agonistic anti-OX40 (CD134) mAb significantly augmented immune responses of draining lymph node cells, but did not augment the therapeutic effect of trimAb. CD4 T cell depletion reduced the antitumor effect of anti-CTLA-4 mAb treatment alone, but did not reduce the tumor rejection rate of trimAb in conjunction with anti-CTLA-4 mAb. Thus, the blockade of the CTLA-4-mediated inhibitory signal in tumor infiltrating CTL may be the most effective strategy to augment the effect of immune therapies that generate tumor-specific CTL.
Thermal Stress Distribution in Thick Wall Cylinder Under Thermal Shock (2009)

This paper presented the stress distribution in a thick walled cylinder under thermal shock. Dirac function was introduced to model thermal shock. An analytical solution of the temperature field was obtained by Laplace transform. Based on the temperature solution, the thermal stress response of the thick walled cylinder was solved. The time dependent variations of the temperature field—thermal stress field—were discussed, and the effect of cylinder radius ratio on the problem was given. The exploration in this paper will lay a theoretical reference to further study on the decrease in fatigue damage of the superhigh pressure tubular reactor under thermal shock.
Activation of Immature Hepatic NK Cells As Immunotherapy for Liver Metastatic Disease (2007)

NK cells can identify and eliminate emerging tumors due to altered expression of activating and inhibitory ligands on aberrant cells, a process that is greatly enhanced following NK cell activation. As a principal site of both tumor metastases and immature NK cells, the liver represents a unique anatomic location in which activation of the innate immune system could provide substantial therapeutic benefit. We describe here the NK cell-dependent destruction of a primary hepatic tumor following infection with an attenuated intracellular bacterium derived from Listeria monocytogenes. NK cell-mediated immunity correlated with the ordered migration and maturation of NK cells within the liver. Cytolytic activity was partially dependent on NKG2D-mediated tumor cell recognition, but surprisingly was still effective in the absence of type I IFN. Significantly, NK cell-mediated destruction of a primary hepatic tumor in infected mice led to long-lived CD4- and CD8 T cell-dependent tumor-specific adaptive immunity. These findings establish that activation and differentiation of immature NK cells using complex microbial stimuli can elicit potent anti-tumor activity within the liver, promote cross-presentation of tumor-derived Ags leading to long-lived systemic anti-tumor immunity, and suggests a paradigm for clinical intervention of liver metastatic carcinoma.
The Efficacy and Safety of Liquid-Type Botulinum Toxin Type A for the Management of Moderate to Severe Glabellar Frown Lines (2015)

Therapeutic, II.
Immunological measurement of transforming growth factor-beta 1 (TGF-beta1) in blood; assay development and comparison. (1997)

Development of a new, sensitive immunoassay for measuring transforming growth factor beta 1 (TGF-beta1) is described and compared with four commercially available TGF-beta1 immunoassays. Preanalytical conditions were evaluated. The nonlinearity found in serum or plasma is due to masking of TGF-beta1 by binding proteins in blood. Mixing TGF-beta1 with latency-associated peptide or alpha2-macroglobulin at physiological concentrations suppressed most of the TGF-beta1 signal. Plasma fibronectin showed no effect, even at concentrations exceeding its physiological range. Equilibrium concentrations computed from a model system confirmed the experimental results. Dilutional nonlinearity could be markedly reduced by an appropriately designed activation procedure that minimized the effects of reassociation between TGF-beta1 and its binding partners during restoration of a neutral pH. Plasma should be used for measuring TGF-beta1 in blood. Because serum TGF-beta1 is highly significantly correlated with the platelet count, probably most of the TGF-beta1 is released by platelet degranulation.
Graphene oxide–chitosan composite hydrogels as broad-spectrum adsorbents for water purification (2012)

Water pollution is one of the most pervasive problems afflicting people throughout the world, while adsorption is the most widely used method to remove the contaminants from water. Here, in this paper, we report an eco-friendly graphene oxide–chitosan (GO–CS) hydrogel as a new type of adsorbent for water purification. The GO–CS hydrogels were prepared via self-assembly of GO sheets and CS chains. A three-dimensional network composed of GO sheets crosslinked by CS was found in GO–CS hydrogels. The GO–CS composite hydrogels showed high adsorption capacity towards different contaminants, including cationic and anionic dyes, as well as heavy metal ions. The mechanism of the dye adsorption was investigated with a spectral method, and an electrostatic interaction was found to be the major interaction between ionic dyes and the hydrogel. The influence of the hydrogel composition on the adsorption capacity towards different adsorbates was also studied. Finally, it was demonstrated that the GO–CS hydrogel can be used as column packing, to fabricate a column for water purification by filtration.
Automated Web Patrol with Strider HoneyMonkeys: Finding Web Sites That Exploit Browser Vulnerabilities. (2006)

Internet attacks that use Web servers to exploit browser vulnerabilities to install malware programs are on the rise [D04,R04,B04,S05]. Several recent reports suggested that some companies may actually be building a business model around such attacks [IF05,R05]. Expensive, manual analyses for individually discovered malicious Web sites have recently emerged [F04,G05]. In this paper, we introduce the concept of Automated Web Patrol, which aims at significantly reducing the cost for monitoring malicious Web sites to protect Internet users. We describe the design and implementation of the Strider HoneyMonkey Exploit Detection System [L05,N05], which consists of a network of monkey programs running on virtual machines with different patch levels and constantly patrolling the Web to hunt for Web sites that exploit browser vulnerabilities. Within the first month of utilizing this new system, we identified 752 unique URLs that are operated by 287 Web sites and that can successfully exploit unpatched WinXP machines. The system automatically constructs topology graphs that capture the connections between the exploit sites based on traffic redirection, which leads to the identification of several major players who are responsible for a large number of exploit pages.
Chemically Modified Electrodes by Nucleophilic Substitution of Chlorosilylated Platinum Oxide Surfaces (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemically Modified Electrodes by Nucleophilic Substitution of Chlorosilylated Platinum Oxide SurfacesC. Chen, James E. Hutchison, Timothy A. Postlethwaite, John N. Richardson, and Royce W. MurrayCite this: Langmuir 1994, 10, 9, 3332–3337Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September 1994https://pubs.acs.org/doi/10.1021/la00021a069https://doi.org/10.1021/la00021a069research-articleACS PublicationsRequest reuse permissionsArticle Views190Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Studies of aggregated gamma-globulin. I. Sedimentation, electrophoretic and anticomplementary properties. (1960)

Summary Molecular aggregation occurs when solutions of γ-globulin are heated to 56°C. Aggregated γ-globulin, when concentrated by salt fractionation, demonstrated marked anticomplementary properties. Preliminary C′ studies suggested that absorption of guinea pig serum with solid γ-globulin resulted in C′ component destruction that resembled C′ inactivation by immune systems.
Involvement of Nitric Oxide in Biofilm Dispersal of Pseudomonas aeruginosa (2006)

Bacterial biofilms at times undergo regulated and coordinated dispersal events where sessile biofilm cells convert to free-swimming, planktonic bacteria. In the opportunistic pathogen Pseudomonas aeruginosa, we previously observed that dispersal occurs concurrently with three interrelated processes within mature biofilms: (i) production of oxidative or nitrosative stress-inducing molecules inside biofilm structures, (ii) bacteriophage induction, and (iii) cell lysis. Here we examine whether specific reactive oxygen or nitrogen intermediates play a role in cell dispersal from P. aeruginosa biofilms. We demonstrate the involvement of anaerobic respiration processes in P. aeruginosa biofilm dispersal and show that nitric oxide (NO), used widely as a signaling molecule in biological systems, causes dispersal of P. aeruginosa biofilm bacteria. Dispersal was induced with low, sublethal concentrations (25 to 500 nM) of the NO donor sodium nitroprusside (SNP). Moreover, a P. aeruginosa mutant lacking the only enzyme capable of generating metabolic NO through anaerobic respiration (nitrite reductase, DeltanirS) did not disperse, whereas a NO reductase mutant (DeltanorCB) exhibited greatly enhanced dispersal. Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilms and biofilm-related infections. We observed that exposure to SNP (500 nM) greatly enhanced the efficacy of antimicrobial compounds (tobramycin, hydrogen peroxide, and sodium dodecyl sulfate) in the removal of established P. aeruginosa biofilms from a glass surface. Combined exposure to both NO and antimicrobial agents may therefore offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections.
Plaque Gruel of Atheromatous Coronary Lesion May Contribute to the No-Reflow Phenomenon in Patients With Acute Coronary Syndrome (2002)

No-reflow after angioplasty may be caused by gruel that formed from an atheroma attributable to mechanical plaque disruption during intervention.
Efficient inverted polymer solar cells (2006)

We investigate the effect of interfacial buffer layers—vanadium oxide (V2O5) and cesium carbonate (Cs2CO3)—on the performance of polymer solar cells based on regioregular poly-(3-hexylthiophene) and [6,6]-phenyl C60 butyric acid methyl ester blend. The polarity of solar cells can be controlled by the relative positions of these two interfacial layers. Efficient inverted polymer solar cells were fabricated with the structure of indium tin oxide (ITO)/Cs2CO3/polymer blend/vanadium oxide (V2O5)/aluminum (Al). Short-circuit current of 8.42mA∕cm2, open-circuit voltage of 0.56V, and power conversion efficiency of 2.25% under a AM1.5G 130mW∕cm2 condition were achieved. The interfacial layers were also used to fabricate polymer solar cells using ITO and a thin gold (Au) layer as the transparent electrodes. The thickness of V2O5 layer (10nm) makes it an effective protective layer for the active layer so that ITO can be used for both the electrodes, enabling highly efficient transparent polymer solar cells (i.e., polymer solar cells with transparent electrodes). Application of this structure for multiple-stacking polymer solar cells is also discussed.
Persistent Activation of c-Jun N-terminal Kinase 1 (JNK1) in γ Radiation-induced Apoptosis (1996)

The c-Jun N-terminal kinases (JNK) are activated by various stimuli, including UV light, interleukin-1, tumor necrosis factor-alpha (TNF-alpha), and CD28 costimulation. Induction of JNK by TNF-alpha, a strong apoptosis inducer, implies a possible role of JNK in the regulation of programmed cell death. Present studies show that lethal doses of gamma radiation (GR) induced JNK activities at the early phase of apoptosis in Jurkat T-cells. We demonstrate that JNK1 was activated by either the T-cell activation signals, anti-CD28 monoclonal antibody plus phorbol 12-myristate 13-acetate (PMA), or the apoptosis-inducing treatment, GR; however, the induction patterns were different. In contrast to the rapid and transient JNK1 activation caused by CD28 signaling plus PMA, GR induced a delayed and persistent JNK1 activation. This implies a distinct regulatory mechanism and specific function of JNK1 in irradiated cells. The nuclear and cytosolic JNK1 activities were simultaneously increased in the irradiated cells without an evident change in the protein levels. The abilities of GR to induce JNK1 activation and DNA fragmentation were correlated. Peripheral blood lymphocytes were more sensitive to GR than Jurkat cells in JNK1 induction. The responsiveness of JNK1 to GR suggests the involvement of JNK1 in the initiation of the apoptosis process.
Systemicin vivodistribution of activatable cell penetrating peptides is superior to that of cell penetrating peptides (2009)

Cell penetrating peptides (CPPs) have been developed as vehicles for payload delivery into cells in culture and in animals. However several biologic features limit their usefulness in living animals. Activatable cell penetrating peptides (ACPPs) are polycationic CPPs whose adsorption and cellular uptake are minimized by a covalently attached polyanionic inhibitory domain. Cleavage of the linker connecting the polyanionic and polycationic domains by specific proteases (tumor associated matrix metalloproteases discussed herein) dissociates the polyanion and enables the cleaved ACPP to enter cells. In contrast to their CPP counterpart, ACPPs are relatively nonadherent and distributed uniformly to normal tissues. While nonaarginine (r(9)) CPP administered intravenously into mice initially bind to the local vasculature and redistribute to the liver, where >90% of the injected dose accumulates 30 min after injection. Regardless of the presence of the polyanionic inhibitory domain, confocal imaging of live tissues reveals that the majority of the ACPP and CPP remain in punctate organelles, presumably endosomes. Therefore further improvements in the efficiency of delivery to the cytosol and nucleus are necessary. In addition to improved target specificity, a major advantage of ACPPs over CPPs for potential clinical applications is reduced toxicity. Systemically administered r(9) CPP causes acute toxicity in mice at a dose 4-fold lower than the MMP cleavable ACPP, a complication not observed with an uncleavable ACPP presumably because the polycationic charge remains masked systemically. These data suggest that ACPPs have greater potential than CPPs for systemic delivery of imaging and therapeutic agents.
Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. (1993)

NCCIT is a developmentally pluripotent cell line that can differentiate into derivatives of all three embryonic germ layers (i.e., ectoderm, mesoderm, and endoderm) and extraembryonic cell lineages. We suggest that this cell line could be a malignant replica of human cleavage stage embryonic cells with features intermediate between seminoma and embryonal carcinoma.
Contribution of NADH Oxidase to Aerobic Metabolism of Streptococcus pyogenes (2000)

ABSTRACT An understanding of how the heme-deficient gram-positive bacterium Streptococcus pyogenes establishes infections in O 2 -rich environments requires careful analysis of the gene products important in aerobic metabolism. NADH oxidase (NOXase) is a unique flavoprotein of S. pyogenes and other lactic acid bacteria which directly catalyzes the four-electron reduction of O 2 to H 2 O. To elucidate a putative role for this enzyme in aerobic metabolism, NOXase-deficient mutants were constructed by insertional inactivation of the gene that encodes NOXase. Characterization of the resulting mutants revealed that growth in rich medium under low-O 2 conditions was indistinguishable from that of the wild type. However, the mutants were unable to grow under high-O 2 conditions and demonstrated enhanced sensitivity to the superoxide-generating agent paraquat. Mutants cultured in liquid medium under conditions of carbohydrate limitation and high O 2 tension were characterized by an extended lag phase, a reduction in growth, and a greater accumulation of H 2 O 2 in the growth medium compared to the wild-type strain. All of these mutant phenotypes could be overcome by the addition of glucose. Either the addition of catalase to the culture medium of the mutants or the introduction of a heterologous NADH peroxidase into the mutants eliminated the accumulation of H 2 O 2 and rescued the growth defect of the mutants under high-O 2 conditions in carbohydrate-limited liquid medium. Taken together, these data show that NOXase is important for aerobic metabolism and essential in environments high in O 2 with carbohydrate limitation.
Stimulation of protective CD8+ T lymphocytes by vaccination with nonliving bacteria. (1995)

Infectious diseases caused by intracellular microbes are responsible for major health problems, and satisfactory control will ultimately depend on efficient vaccination strategies. The general assumption is that activation of protective immune responses against intracellular microbes dominated by CD8+ T cells are achieved only by live vaccines. In contrast, we here demonstrate stimulation of protective immunity in mice against the intracellular pathogen Listeria monocytogenes by vaccination with heat-killed listeriae. Vaccine-induced immunity comprised cytolytic and interferon gamma-producing CD8+ T lymphocytes. CD8+ T cells from vaccinated donor mice transferred protection against listeriosis. Moreover, vaccination with heat-killed listeriae induced production in CD4+ T-cell-deficient, H2-A beta gene-disrupted mutant mice. We conclude that antigens from killed listeriae are introduced into the major histocompatibility complex class I pathway and thus are recognized by CD8+ T cells. The practicability of killed vaccines against human infectious diseases therefore should be reevaluated.
Substituted N-phenylcarbamates as histamine H3 receptor antagonists with improved in vivo potency. (2000)

Novel substituted N-phenylcarbamates as derivatives of 3-(1 H-imidazol-4-yl)propanol were prepared and tested for their antagonist potency in vitro and in vivo at histamine H3 receptors. Structural modifications with different alkyl and acetyl moieties were performed in an attempt to optimize pharmacodynamic and pharmacokinetic effects. Most compounds are active in a functional test for histamine H3 receptors on rat cerebral cortex synaptosomes as well as in a peripheral model on guinea pig ileum. But only carbamates without too bulky lipophilic residues showed pronounced to high antagonist potency on the enhancement of endogenous histamine in brain after p.o. administration to mice (ED50 values of 5.5 to 0.86 mg.kg-1). The tested compounds presented weak activities at histamine H1, H2, and muscarinic M3 receptors thus demonstrating their H3-receptor selectivity.
Viruses and bone marrow : basic research and clinical practice (1993)

Part 1 General aspects of virus infection of haematopoietic cells: mechanisms of viral pathogenesis, Raymond M. Welsh and Hugh I. McFarland immune response to viral infection, Caroline I.B. Kurtz and Robert S. Fujinami. Part 2 Parvoviruses: B19 parvoviruses, Neal S. Young feline panleukopenia virus, Gary Kurtzman. Part 3 Herpesviruses: Epstein-Barr virus, Bruce G. Baranski and Ian T. Magrath human cytomegalovirus, Stephen St Jeor and Jaroslaw P. Maciejewski. Part 4 Flaviviruses: Dengue virus and haematopoiesis, Stephen J. Rosenfeld et al hepatitis viruses and bone marrow depression, Pascal Bouffard and Jerome B. Zeldis. Part 5 Retroviruses: human immunodeficiency virus and the bone marrow, Jerry L. Spivak and Barbara Potts friend spleen focus-forming virus, Sandra K. Ruscetti feline leukaemia virus, Janis L. Abkowitz and Michael L. Linenberger. Part 6 Viruses as vectors for gene therapy: viruses as molecular tools, Arthur W. Nienhuis et al.