gebhart/patrepeval huggingface dataset:
A platelet alpha granule membrane protein that is associated with the plasma membrane after activation. Characterization and subcellular localization of platelet activation-dependent granule-external membrane protein. (1986)

We have identified and purified a platelet integral membrane protein (140,000 mol wt), using the KC4 monoclonal antibody specific for activated platelets, that is internal in resting platelets but exposed on activated platelets (Hsu-Lin S.-C., C.L. Berman, B.C. Furie, D. August, and B. Furie, 1984, J. Biol. Chem. 259: 9121-9126.). The expression of the protein on the platelet surface is secretion-dependent. This protein has been named platelet activation-dependent granule-external membrane (PADGEM) protein. PADGEM protein is distinct from the surface glycoproteins of resting platelets, but identical to the S12 antigen, GMP-140. Using immunofluorescent staining, resting platelets failed to stain for PADGEM protein with the KC4 antibody, but after permeabilization showed a punctate staining of the cell interior. Thrombin-stimulated intact platelets stained with a peripheral rim pattern thus demonstrating the translocation of PADGEM protein from an internal location to the cell surface. PADGEM protein expression on the platelet surface at varying thrombin concentrations correlated with alpha granule release, as measured by the secretion of platelet factor 4. Further evidence for an alpha granule localization of PADGEM protein was provided by nitrogen cavitation of resting platelets followed by metrizamide density gradient centrifugation; PADGEM protein codistributed with platelet factor 4. Using immunoelectron microscopy, the protein was localized to the alpha granule in frozen ultrathin sections of resting platelets labeled using rabbit anti-PADGEM protein antibodies, whereas in thrombin-activated platelets, the plasma membrane was labeled. These studies indicate that PADGEM protein is a component of the alpha granule membrane of resting platelets and is incorporated into the plasma membrane upon activation and secretion.
Interactions That Influence the Binding of Synthetic Heparan Sulfate Based Disaccharides to Fibroblast Growth Factor-2 (2014)

Heparan sulfate (HS) is a linear sulfated polysaccharide that mediates protein activities at the cell-extracellular interface. Its interactions with proteins depend on the complex patterns of sulfonations and sugar residues. Previously, we synthesized all 48 potential disaccharides found in HS and used them for affinity screening and X-ray structural analysis with fibroblast growth factor-1 (FGF1). Herein, we evaluated the affinities of the same sugars against FGF2 and determined the crystal structures of FGF2 in complex with three disaccharides carrying N-sulfonated glucosamine and 2-O-sulfonated iduronic acid as basic backbones. The crystal structures show that water molecules mediate different interactions between the 3-O-sulfonate group and Lys125. Moreover, the 6-O-sulfonate group forms intermolecular interactions with another FGF2 unit apart from the main binding site. These findings suggest that the water-mediated interactions and the intermolecular interactions influence the binding affinity of different disaccharides with FGF2, correlating with their respective dissociation constants in solution.
Analysis of the human CD10/neutral endopeptidase 24.11 promoter region: two separate regulatory elements (1995)

The cell surface zinc metalloproteinase CD10/neutral endopeptidase 24.11 (NEP) is expressed on normal and malignant lymphoid progenitors, granulocytes, and a variety of epithelial cells. To further define the tissue-specific and developmentally related expression of CD10/NEP, we have characterized two separate regulatory regions that control the transcription of 5' alternatively spliced CD10/NEP transcripts. These type 1 and 2 CD10/NEP regulatory regions are both characterized by the presence of multiple transcription initiation sites and the absence of classic TATA boxes and consensus initiator elements. The purine-rich type 1 regulatory region, which includes 5' UTR exon 1 sequence, is characterized by multiple putative PU.1 binding sites and consensus ets-binding motifs. In marked contrast, the GC-rich type 2 regulatory region contains multiple putative Sp1 binding sites, a potential consensus retinoblastoma control element (RCE), and an inverted CCAAT box. In the majority of tissues examined to date, type 2 CD10/NEP transcripts were more abundant; the abundance of type 1 transcripts was more variable, with the highest type 1 levels in fetal thymus and certain lymphoblastic leukemia cell lines.
Experimental Measurement of the Rate of Methyl tert-Butyl Ether Hydrolysis in Sub- and Supercritical Water (2000)

Experiments were conducted to measure the isothermal rate of methyl tert-butyl ether (MTBE) decomposition in water at hydrothermal conditions (T = 150−600 °C and P = 250 bar). The primary identified products of the reaction of MTBE were methanol, isobutene, and tert-butyl alcohol. An assumed first-order rate constant for the hydrolysis of MTBE was determined at each temperature and showed a local maximum below the critical temperature of water (374 °C) followed by a local minimum above it. This behavior was modeled using an acid-catalyzed mechanism, which resulted in a rate expression with a first-order dependence on the concentration of H+. Because of the dramatic decrease in the ion dissociation constant of water (Kw) in the critical region, the drop in the rate of reaction could be modeled quantitatively over the entire temperature range.
Research on a high energy utilization efficiency electromagnetic aircraft launcher with an ultracapacitor bank (2008)

Energy utilization efficiency (EUE) is low when an ultracapacitor bank directly supplies power for an electromagnetic aircraft launcher (EMAL). To solve this problem, a boost converter is added between the ultracapacitor bank and the linear acceleration motor of the EMAL. The difference between the terminal voltage of the ultracapacitor bank and the peak back electromotive force of the linear motor is used to control the boost converter. System modeling and parametric design are presented. Simulation results show the ultracapacitor bank can discharge fast over a wide terminal voltage range and EUE is improved remarkably.
Assessment of Various Cell Lines (Including Mixed-Cell Cultures) for the Detection of Enteric Viruses in Different Water Sources (1989)

Research Article| March 01 1989 Assessment of Various Cell Lines (Including Mixed-Cell Cultures) for the Detection of Enteric Viruses in Different Water Sources D. Tougianidou; D. Tougianidou Search for other works by this author on: This Site PubMed Google Scholar M. Jacob; M. Jacob Search for other works by this author on: This Site PubMed Google Scholar K. Herbold; K. Herbold Search for other works by this author on: This Site PubMed Google Scholar T. Hahn; T. Hahn Search for other works by this author on: This Site PubMed Google Scholar B. Flehmig; B. Flehmig Search for other works by this author on: This Site PubMed Google Scholar K. Botzenhart K. Botzenhart Search for other works by this author on: This Site PubMed Google Scholar Water Sci Technol (1989) 21 (3): 311–314. https://doi.org/10.2166/wst.1989.0126 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Cite Icon Cite Permissions Search Site Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsThis Journal Search Advanced Search Citation D. Tougianidou, M. Jacob, K. Herbold, T. Hahn, B. Flehmig, K. Botzenhart; Assessment of Various Cell Lines (Including Mixed-Cell Cultures) for the Detection of Enteric Viruses in Different Water Sources. Water Sci Technol 1 March 1989; 21 (3): 311–314. doi: https://doi.org/10.2166/wst.1989.0126 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex This content is only available as a PDF. © IWA Publishing 1989 Article PDF first page preview Close Modal You do not currently have access to this content.
초등학교 1학년 학생의 독서마라톤 참여 경험에 대한 연구 (2018)

학교 현장에서는 다양한 종류의 독서 프로그램이 기획되어 운영되고 있다. 본 연구에서는 2017년 전북 전주 소재 공립초등학교에서 실시한 독서마라톤과 관련하여, 초등학교 1학년 학생들이 그 독서마라톤을 어떻게 경험하였는지, 그 영향이 어떠한 지를 탐색하였다. 연구 참여자는 독서마라톤 참여 태도가 우수한 초등학교 1학년 학습자 3명, 그들의 학부모 3명,...
Adsorptive-mediated endocytosis of a basic peptide in enterocyte-like Caco-2 cells (1998)

The internalization of a basic peptide, 001-C8 [H-MeTyr-Arg-MeArg-D-Leu-NH(CH2)8NH2], into enterocyte-like Caco-2 cells was evaluated. Internalization of 125I-labeled 001-C8 (125I-001-C8) increased time dependently and reached steady state at 60 min. The steady-state internalization of 125I-001-C8 (7.24 +/- 0. 41 microl/mg protein) was temperature and concentration dependent and was significantly decreased by dansylcadaverine (500 microM), protamine (1 mM), poly-L-lysine (1 mM), E-2078 (1 mM), and ebiratide (1 mM), whereas poly-L-glutamic acid (1 mM), tyrosine (1 mM), and glycylglycine (25 mM) were not inhibitory. Predigestion of acid mucopolysaccharides by heparinase I, heparitinase, and chondroitinase ABC also decreased the internalization. The maximal internalization, the half-saturation constant, and the nonsaturable internalization of 125I-001-C8 were 1.13 +/- 0.23 pmol/mg protein, 0. 47 +/- 0.43 microM, and 3.13 +/- 0.19 microl/mg protein, respectively. Confocal microscopy also indicated the internalization of fluorescence-derived 001-C8 [001-C8-4-nitrobenz-2-oxa-1,3-diazole (001-C8-NBD)]. Granular staining seen within the cell, excluding nuclei, indicated the sequestration of 001-C8-NBD within endocytotic vesicles. Dansylcadaverine and protamine strongly decreased the granular distribution of 001-C8-NBD within the cell. These results demonstrate that 001-C8 is taken up by Caco-2 cells via adsorptive-mediated endocytosis.
Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation (2009)

The proteasome degrades proteins modified by polyubiquitylation, so correctly controlled ubiquitylation is crucial to avoid unscheduled proteolysis of essential proteins. The mechanism regulating proteolysis of RNAPII has been controversial since two distinct ubiquitin ligases (E3s), Rsp5 (and its human homologue NEDD4) and Elongin-Cullin complex, have both been shown to be required for its DNA-damage-induced polyubiquitylation. Here we show that these E3s work sequentially in a two-step mechanism. First, Rsp5 adds mono-ubiquitin, or sometimes a ubiquitin chain linked via ubiquitin lysine 63 that does not trigger proteolysis. When produced, the K63 chain can be trimmed to mono-ubiquitylation by an Rsp5-associated ubiquitin protease, Ubp2. Based on this mono-ubiquitin moiety on RNAPII, an Elc1/Cul3 complex then produces a ubiquitin chain linked via lysine 48, which can trigger proteolysis. Likewise, for correct polyubiquitylation of human RNAPII, NEDD4 cooperates with the ElonginA/B/C-Cullin 5 complex. These data indicate that RNAPII polyubiquitylation requires cooperation between distinct, sequentially acting ubiquitin ligases, and raise the intriguing possibility that other members of the large and functionally diverse family of NEDD4-like ubiquitin ligases also require the assistance of a second E3 when targeting proteins for degradation.
Antibodies that block rhinovirus attachment map to domain 1 of the major group receptor (1990)

The vast majority of human rhinovirus serotypes utilize the intercellular adhesion molecule 1 (ICAM-1) as the attachment site on susceptible cells. Twelve murine monoclonal antibodies were isolated and shown by competition binding studies to recognize three distinct, nonoverlapping epitopes on the ICAM-1 receptor. Titration of three antibodies representing each of the binding sites demonstrated that they were equally effective at blocking viral attachment. By using in vitro transcription and translation systems, a series of progressive C-terminal truncations of ICAM-1 molecules was generated. Immunoprecipitation of these fragments with each of the three antibodies indicated that all three epitopes reside within the first 82 amino acids of the receptor. Attempts to demonstrate specific binding of these in vitro-synthesized receptor fragments to virions were unsuccessful. The inability to show virion binding was most likely due to a failure of the lysates to properly glycosylate the receptor molecule, since native, unglycosylated receptor molecules isolated from cell membranes were also inactive in virus binding assays.
Pseudomonas aeruginosa ExoS ADP-ribosyltransferase inhibits ERM phosphorylation (2006)

Pseudomonas aeruginosa causes life-threatening infections in compromised and cystic fibrosis patients. Pathogenesis stems from a number of virulence factors, including four type III translocated cytotoxins: ExoS, ExoT, ExoY and ExoU. ExoS is a bifunctional toxin: the N terminus (amino acids 96-219) encodes a Rho GTPase Activating Protein (GAP) domain. The C terminus (amino acids 234-453) encodes a 14-3-3-dependent ADP-ribosyltransferase domain which transfers ADP-ribose from NAD onto substrates such as the Ras GTPases and vimentin. Ezrin/radixin/moesin (ERM) proteins have recently been identified as high-affinity substrates for ADP-ribosylation by ExoS. Expression of ExoS in HeLa cells led to a loss of phosphorylation of ERM proteins that was dependent upon the expression of ADP-ribosyltransferase activity. MALDI-MS and site-directed mutagenesis studies determined that ExoS ADP-ribosylated moesin at three C-terminal arginines (Arg553, Arg560 and Arg563), which cluster Thr558, the site of phosphorylation by protein kinase C and Rho kinase. ADP-ribosylated-moesin was a poor target for phosphorylation by protein kinase C and Rho kinase, which showed that ADP-ribosylation directly inhibited ERM phosphorylation. Expression of dominant active-moesin inhibited cell rounding elicited by ExoS, indicating that moesin is a physiological target in cultured cells. This is the first demonstration that a bacterial toxin inhibits the phosphorylation of a mammalian protein through ADP-ribosylation. These data explain how the expression of the ADP-ribosylation of ExoS modifies the actin cytoskeleton and indicate that ExoS possesses redundant enzymatic activities to depolymerize the actin cytoskeleton.
B-Cell Chronic Lymphocytic Leukemia Cells Express a Functional Inducible Nitric Oxide Synthase Displaying Anti-Apoptotic Activity (1998)

The expression of different isoforms of nitric oxide synthase (NOS) was investigated in B-cell chronic lymphocytic leukemia (B-CLL) to delineate a possible role for nitric oxide (NO) in the control of apoptosis of the tumoral cells. By reverse transcription-polymerase chain reaction (RT-PCR), all B-CLL cells were found to express spontaneously inducible NOS (iNOS) mRNA, whereas endothelial constitutive NOS (ecNOS) mRNA was undetectable. The iNOS protein was detected by immunofluorescence in the cytoplasm of permeabilized leukemic cells and identified by Western blotting, using different anti-iNOS antibodies, as a protein of 135 kD in B-CLL cytoplasmic extracts. B-CLL cell lysates also displayed basal NOS enzymatic activity, as measured by the conversion of 14C-labeled L-arginine into 14C-L-citrulline. Ligation of CD23, expressed on the vast majority of B-CLL cells, resulted in increased iNOS expression and activity. The NO released exerted an anti-apoptotic effect on B-CLL cells that was counteracted by NOS inhibitors and engagement of the APO-1/Fas pathway. Therefore, the existence of a functional iNOS in B-CLL cells will provide further insights into the mechanisms that control proliferation and apoptosis in these tumor cells.
Real time Hand Gesture Recognition using a Range Camera (2009)

This paper proposes a real time hand gesture recognition system. The approach uses a range camera to capture the depth data. After some preprocessing procedures, the depth data is used to segment the hand and then locate the hand in 3D space. The hand shape is classified into known categories using a chamfer matching method to measure the similarities between the candidate hand image and the hand templates in the database. The 3D hand trajectory is recognized by a Finite State Machine (FSM) method. Each gesture consists of several states. The 3D hand position determines the state transition of each gesture recognizer. Experiments show that the system performs reliably for recognizing both static hand shapes and spatial-temporal trajectories in real time.
Prolongation of Canine Epidural Anesthesia by Liposome Encapsulation of Lidocaine (1992)

The purpose of our study was to produce a long-acting lidocaine by using a liposome that would entrap the drug. Egg yolk phosphatidylcholine and cholesterol were used as liposome materials. After epidural administration, the pharmacodynamics and pharmacokinetics of liposomal and free lidocaine were studied in 20 dogs. Two percent liposomal or free lidocaine (3.0 mL) was injected into the lumbar epidural space. Nerve blocking effects were estimated by measuring somatosensory evoked potentials. Recovery time from the epidural block in the liposomal lidocaine group (170 +/- 49.5 min) was approximately three times longer than that in the free lidocaine group (61 +/- 18.1 min). The areas under the drug concentration-time curves (AUC0-infinity) and time to maximal concentration (Tmax) in the liposomal lidocaine group were significantly larger than those in the free lidocaine group. These results suggest that the prolongation of epidural blockade by liposomal lidocaine is caused by a slow release of the drug from liposomes. The present study suggests that liposomal lidocaine can be used as a long-acting local anesthetic.
Biomarkers of insulin sensitivity and insulin resistance: Past, present and future (2015)

Insulin resistance in insulin target tissues including liver, skeletal muscle and adipose tissue is an early step in the progression towards type 2 diabetes. Accurate diagnostic parameters reflective of insulin resistance are essential. Longstanding tests for fasting blood glucose and HbA1c are useful and although the hyperinsulinemic euglycemic clamp remains a "gold standard" for accurately determining insulin resistance, it cannot be implemented on a routine basis. The study of adipokines, and more recently myokines and hepatokines, as potential biomarkers for insulin sensitivity is now an attractive and relatively straightforward approach. This review discusses potential biomarkers including adiponectin, RBP4, chemerin, A-FABP, FGF21, fetuin-A, myostatin, IL-6, and irisin, all of which may play significant roles in determining insulin sensitivity. We also review potential future directions of new biological markers for measuring insulin resistance, including metabolomics and gut microbiome. Collectively, these approaches will provide clinicians with the tools for more accurate, and perhaps personalized, diagnosis of insulin resistance.
Off-pump transapical mitral valve replacement☆ (2009)

Implantation of a tricuspid bovine pericardial valved stent in the mitral position is feasible in pigs through a transcatheter approach. This was possible through a smaller delivery system than previously reported. Additional studies are required to demonstrate long-term feasibility, durability, and heart function.
Importance of albumin in cross-reactivity among cat, dog and horse allergens. (2000)

Different allergenic proteins have been involved in cross-reactivity among animals. Albumins seem to be cross-sensitizing allergenic components. The aim of this study was to assess the importance of albumin as a cross-reactive allergen in patients sensitized to cat, dog and horse. One hundred and seventeen patients sensitized to cat were tested for IgE reactivity using skin prick tests and RAST assays with cat, dog and horse hair/dander extracts and their purified albumin extracts. RAST-inhibition studies were carried out to assess cross-reactivity among cat, dog and horse and among their purified albumins. It was found that 22% of patients exhibited specific IgE to cat albumin; 41% of patients sensitized to cat were also sensitized to dog and horse. Out of these patients, 21% had IgE to three albumins and 17% to two. Reciprocal inhibitions were observed among cat, dog and horse albumins and also among cat, dog and horse hair/dander extracts, using in the latter experiment sera from patients not sensitized to albumins. IgE binding to horse extract was inhibited 30% by its homologous albumin and IgE binding to cat and dog extracts in almost 15% by their respective albumins. It was concluded that albumins from these three animals share some epitopes that account for the cross-reactivity observed in around one-third of patients sensitized to cat, dog and horse. Nevertheless, more than 50% of specific IgE that cross-reacts among these three animals is directed to allergens other than albumin.
Development-dependent UV-B Responses in Red Oak Leaf Lettuce (Lactuca sativa L.): Physiological Mechanisms and Significance for Hardening (2011)

Plants protect themselves against potentially harmful UV-B radiation mainly by epidermal accumulation of UV-B shielding phenolic compounds and repair of cellular damage. The capacity and necessity for these protection mechanisms alter with the stage of plant and leaf development, which might be crucial for horticultural hardening techniques aiming at a UV-B pre-acclimation of greenhouse grown seedlings before transplantation to the field. In order to examine the development-dependence of UV-B responses and to estimate the efficiency of different hardening strategies, lettuce (Lactuca sativa L., var. crispa L. `Bughatti') was raised in greenhouses covered with a UV-B transmitting and a UV-B excluding covering material and transferred from one treatment to the other at the age of 20 days. Plant fresh mass and flavonoid content were frequently determined. Distribution of flavonoids within the plants was assessed by extraction of different leaf age classes. Continuous exposure to near ambient UV-B induced a strong enhancement in cyanidin and quercetin content and a severe growth reduction, whereas late UV-B treatment merely led to a slight enhancement of quercetin in the absence of any growth response. At the leaf level, continuous UV-B exposure caused a comparable quercetin induction at all three stages of leaf development, whereas late treatment caused a much stronger response in young compared to intermediate and old leaves. These findings indicate that UV-B effects on growth and flavonoid content and pattern of lettuce plants are highly dependent on the leaf and plant developmental stage. We conclude that UV-B exposure throughout seedling development is a more efficient hardening strategy than late short-term exposure.
Lack of Diagnostic Utility of Vimentin Immunostaining (1989)

We documented the coexpression of cytokeratins and vimentin in epithelial cells of the thyroid gland in 100 samples examined from 65 patients. These included normal, inflammatory, and neoplastic tissues that were routinely fixed in formalin and embedded in paraffin. The number of epithelial cells coexpressing the two intermediate filament proteins as well as the subcellular compartmentalization of vimentin immunoreactivity did not correlate with the various conditions of the thyroid gland. Therefore, we conclude that the immunolocalization of vimentin does not represent a useful adjunct tool for the histopathological diagnosis of thyroid diseases.