gebhart/patrepeval huggingface dataset:
An RNA aptamer to the xanthine/guanine base with a distinctive mode of purine recognition (1998)

RNAs that bind to xanthine (2,6-dioxypurine) were isolated from a population of 10(12) random sequences by in vitro selection. These xanthine-binding RNAs were found to have a 10 nt consensus sequence at an internal loop in the most probable secondary structure. By trimming one of the xanthine-binding RNAs, a representative xanthine-binding RNA (designated as XBA) of 32 nt residues was prepared. The dissociation constant of this RNA for xanthine was determined to be 3.3 microM by equilibrium filtration experiments. The XBA RNA can bind to guanine as well, whereas it hardly accommodates adenine, cytosine or uracil. The K d values for various xanthine/guanine analogues were determined, and revealed that the N1H, N7 and O6 moieties of the ligand are involved in the binding with the XBA RNA. The ribonuclease sensitivities of some internal-loop residues changed upon the addition of xanthine, suggesting that the internal loop of the XBA RNA is involved in the ligand binding. Interestingly, the consensus sequence of the xanthine/guanine-binding RNAs is the same as a sequence in one of the internal loops of the hairpin ribozyme, except for a substitution that is neutral with respect to xanthine/guanine binding.
Retroviral Coexpression of a Multidrug Resistance Gene ( MDR 1) and Human α -Galactosidase A for Gene Therapy of Fabry Disease (1995)

Human alpha-galactosidase A (alpha-Gal A; EC.3.2.1.22) is a lysosomal exoglycosidase encoded by a gene on Xq22. Deficiencies of this enzyme result in Fabry disease, an X-chromosome-linked recessive disorder that leads to premature death in affected males. For treatment of genetic diseases, we have developed a retroviral vector system, pSXLC/pHa, that enables coexpression of drug-selectable markers with a second nonselectable gene as part of a bicistronic message using the promoter from the Harvey murine sarcoma virus and an internal ribosomal entry site (IRES) from encephalomyocarditis virus. Retroviral vectors based on this system that carry the human alpha-Gal A cDNA either upstream (pHa-alpha Gal-IRES-MDR) or downstream (pHa-MDR-IRES-alpha Gal) from the IRES relative to the drug-selectable MDR1 (P-glycoprotein) cDNA were constructed. Each of eight independent vincristine-resistant, pHa-alpha Gal-IRES-MDR-transfected clones and all four vincristine-resistant, pHa-alpha Gal-IRES-MDR retrovirus-transduced clones showed significantly higher activity of alpha-Gal A than the parental cells. More than 50% of the vincristine-resistant, pHa-MDR-IRES-alpha Gal-transfected clones and all four vincristine-resistant, pHa-MDR-IRES-alpha Gal retrovirus-transduced clones showed significantly higher activity of alpha-Gal A than the parental cells. In these bicistronic vectors, the cDNA whose translation was cap-dependent (upstream) was expressed at higher levels than when the same cDNA was translated in an IRES-dependent manner (downstream). These vectors may prove useful in the gene therapy of Fabry disease.
Use of a direct-fed microbial product as a supplement during the transition period in dairy cattle (2014)

Two studies were conducted. The objective of the first study was to assess the effects of a direct-fed microbial (DFM) product on dry matter intake, milk yield, milk components, disease incidence, and blood metabolites in dairy cattle. The objective of the second study was to assess the effects of DFM on apparent total-tract nutrient digestibility (ATTD). One hundred twenty primiparous and multiparous Holstein cows housed in a tiestall facility at the University of Guelph were used in study 1, and a subset (21) of the same cows participated in study 2. Cows were blocked by anticipated calving date (6 blocks) and then randomly assigned within parity to receive either a DFM supplement (Chr. Hansen Ltd., Milwaukee, WI) or placebo (control). The DFM supplement provided cows with 5.0 × 10(9) cfu/d of 3 strains of Enterococcus faecium and 2.0 × 10(9) cfu/d of Saccharomyces cerevisiae. The DFM supplement was mixed with 0.5 kg of ground dry corn and top-dressed during the morning feeding. The placebo supplement contained the corn only. Individual feed intakes and milk yields were recorded daily. The experiment commenced 3 wk before calving and ended 10 wk postcalving. Milk samples for component analysis were collected on 3 d per week and pooled by week. Body weights and body condition scores were assessed 1 d before enrollment in the study (wk -3), postcalving (wk 1), and at the end of wk 3, 6, and 9. Blood samples were collected before calving (wk -3) and the end of wk 1 and 3. Study 1 showed that treatment had no effect on average dry matter intake or milk yield (kg/d) over the duration of the experiment. The changes in body weights and body condition scores and net energy balance over the duration of the experiment did not differ due to treatment. Treatment had no effect on plasma concentrations of β-hydroxybutyrate, nonesterified fatty acids, glucose, or haptoglobin. Study 2 investigated the effects of DFM on ATTD of starch and neutral detergent fiber (NDF) using insoluble NDF and lignin as internal markers. Study 2 used 21 cows (block 6) from the cows that participated in study 1 while the cows were between 60 and 70 d in milk. Cows receiving DFM had lower fecal starch content (0.88 ± 0.10 vs. 1.39 ± 0.25) and greater ATTD for starch (98.76% ± 0.28 vs. 97.87% ± 0.24) compared with those receiving placebo, and the AATD of NDF did not differ. Additionally, we detected no difference between internal markers for the measurement of ATTD. In conclusion, we were unable to detect a change in overall dry matter intake, milk yield, or milk and blood parameters with DFM supplementation. However, our results demonstrated that DFM can have a positive effect on total-tract starch digestibility. More studies are needed to investigate the effects of DFM and their modes of action under multiple management conditions.
Synthesis and biological activity of five tetrahydrocannabinol metabolites (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and biological activity of five tetrahydrocannabinol metabolitesR. Mechoulam, H. Varconi, Z. Ben-Zvi, H. Edery, and Y. GrunfeldCite this: J. Am. Chem. Soc. 1972, 94, 22, 7930–7931Publication Date (Print):November 1, 1972Publication History Published online1 May 2002Published inissue 1 November 1972https://pubs.acs.org/doi/10.1021/ja00777a049https://doi.org/10.1021/ja00777a049research-articleACS PublicationsRequest reuse permissionsArticle Views353Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Proteobacteria: A Common Factor in Human Diseases (2017)

Microbiota represents the entire microbial community present in the gut host. It serves several functions establishing a mutualistic relation with the host. Latest years have seen a burst in the number of studies focusing on this topic, in particular on intestinal diseases. In this scenario, Proteobacteria are one of the most abundant phyla, comprising several known human pathogens. This review highlights the latest findings on the role of Proteobacteria not only in intestinal but also in extraintestinal diseases. Indeed, an increasing amount of data identifies Proteobacteria as a possible microbial signature of disease. Several studies demonstrate an increased abundance of members belonging to this phylum in such conditions. Major evidences currently involve metabolic disorders and inflammatory bowel disease. However, more recent studies suggest a role also in lung diseases, such as asthma and chronic obstructive pulmonary disease, but evidences are still scant. Notably, all these conditions are sustained by various degree of inflammation, which thus represents a core aspect of Proteobacteria-related diseases.
Core-shell nanoscale coordination polymers combine chemotherapy and photodynamic therapy to potentiate checkpoint blockade cancer immunotherapy (2016)

Advanced colorectal cancer is one of the deadliest cancers, with a 5-year survival rate of only 12% for patients with the metastatic disease. Checkpoint inhibitors, such as the antibodies inhibiting the PD-1/PD-L1 axis, are among the most promising immunotherapies for patients with advanced colon cancer, but their durable response rate remains low. We herein report the use of immunogenic nanoparticles to augment the antitumour efficacy of PD-L1 antibody-mediated cancer immunotherapy. Nanoscale coordination polymer (NCP) core-shell nanoparticles carry oxaliplatin in the core and the photosensitizer pyropheophorbide-lipid conjugate (pyrolipid) in the shell (NCP@pyrolipid) for effective chemotherapy and photodynamic therapy (PDT). Synergy between oxaliplatin and pyrolipid-induced PDT kills tumour cells and provokes an immune response, resulting in calreticulin exposure on the cell surface, antitumour vaccination and an abscopal effect. When combined with anti-PD-L1 therapy, NCP@pyrolipid mediates regression of both light-irradiated primary tumours and non-irradiated distant tumours by inducing a strong tumour-specific immune response.
An effective lateral fiber-optic electronic coupling and packaging technique suitable for VHSIC applications (1986)

As part of a project to develop a high speed (500 MHz) monolithic silicon fiber-optic digital receiver IC, we have produced a novel and practical lateral fiber-detector coupling scheme employing a 45° mirror fabricated on the end of the fiber. Coupling losses of 1.1 dB have been achieved. The technique shows its greatest promise at very high speeds where the IC interconnect issue is emerging as one of utmost relevance. In the work described here, the laterally coupled IC's were packaged in high speed metal flat packs. This technique rendered a fiber-optic digital receiver compatible with two-dimensional layout techniques common in high speed electronics. However, the lateral coupler concept itself is not dependent on the use of metal flat packs. In fact, it is likely to find its greatest utility in the optical coupling of many fibers to a single IC. In this paper, system design considerations, fabrication techniques, and measured results for the lateral coupler concept are discussed.
Statins Are Associated With a Reduced Incidence of Perioperative Mortality After Coronary Artery Bypass Graft Surgery (2004)

Preoperative statin therapy may reduce the risk of early mortality after primary CABG surgery with CPB.
Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service (2003)

The aim of the present study was to develop a broad-range PCR based on bacterial 16S rDNA for use in the routine diagnostic clinical microbiology service. The optimization and validation of the assay for use on clinical specimens from normally sterile sites is described, and preliminary results are reported on the use of the assay in the clinical diagnosis of bacterial infection in 382 paediatric specimens over a 2-year period. These results are compared to those obtained by standard culture techniques and show increased diagnosis of bacterial infection when both culture and PCR are used together; 16S rDNA PCR provided the sole evidence of pathogenic infection in 71 cases. Key stages in the assay development and potential pitfalls of the technique are highlighted and the improvement the assay offers in the diagnosis of infection in the paediatric setting is discussed.
A Homologue of the Mycobacterium tuberculosis PapA5 Protein, Rif‐Orf20, Is an Acetyltransferase Involved in the Biosynthesis of Antitubercular Drug Rifamycin B by Amycolatopsis mediterranei S699 (2005)

Toward antitubercular drugs. Rif-Orf20, a homologue of M. tuberculosis PapA5 protein, is an acetyltransferase that is responsible for the conversion of DMDARSV to DMRSV in rifamycin B biosynthesis. This enzyme, together with PapA5, represents a new class of acyltransferases. The enzyme also utilizes propionyl-CoA as substrate to give rise to a new analogue of rifamycin that appears, from spectroscopic results, to have the structure 1.
Term conflation for information retrieval (1984)

This paper describes two experiments concerned with term conflation for information retrieval, and the CATALOG retrieval system designed utilizing the results of the experiments. The experiments performed here has as their aim 1) finding a theoretical basis and method for maximizing the effect of conflation, and 2) determining if conflation can be automated with no loss of system performance.Experimental results indicate that,1. Experienced searchers generally truncate terms at root morpheme boundaries. When searchers do not truncate at root boundaries, the deviations are small.2. Small deviations from root boundaries do not significantly affect retrieval performance.3. There is no significant performance difference between automatic conflation and manual conflation carried out by experienced searchers.4. Based on 3, term conflation can be automated in a retrieval system with no average loss of performance, thus allowing easier and user access to the system.A retrieval system incorporating the information in 4 is described, and shown to be feasible.
<title>Attenuated phase-shifting mask with a single-layer absorptive shifter of CrO, CrON, MoSiO, and MoSiON film</title> (1994)

Attenuated phase-shifting mask with a single-layer absorptive shifter of CrO, CrON, MoSiO or MoSiON films has been developed. The optical parameter of these films can be controlled by the condition of sputtering deposition. These films satisfy the shifter requirements, both the 180-degrees phase shift and the transmittance between 5 and 20% for i-line. MoSiO and MoSiON films also satisfy the requirement for KrF excimer laser light. Conventional mask processes, such as etching, cleaning, defect inspection and defect repair, can be used for the mask fabrication. Defect-free masks for hole layers of 64 M-bit DRAM are obtained. Using this mask, the focus depth of 0.35-micrometers hole is improved from 0.6 micrometers to 1.5 micrometers for i-line lithography. The printing of 0.2-micrometers hole patterns is achieved by the combination of this mask and KrF excimer laser lithography.
Differential Utilization of Trk Autophosphorylation Sites (1996)

Tyrosine autophosphorylation controls the catalytic and signaling activities of the neurotrophin receptors, the Trks. To analyze the regulation of distinct tyrosine sites, we generated a panel of antibodies that report the phosphorylation state of individual tyrosines within the Trk cytoplasmic domain. Using pheochromocytoma-derived cell lines, we show that individual tyrosines within the nerve growth factor receptor TrkA are phosphorylated in a non-coordinate fashion following receptor activation. The non-coordinate response of these tyrosines reflects their separate functions in regulating the catalytic and signaling activities of Trk receptors. The differential utilization of distinct sites on Trk receptor tyrosine kinases suggests that the receptor can specify both the timing and the nature of neurotrophin-stimulated signal transduction pathways. Moreover, we show that these Trk autophosphorylation sites, which have hitherto been mapped and characterized only in non-neuronal cell lines, are activated in normal neurons in response to ligand stimulation.
Distribution and identification of proteolytic Bacillus spp. in paddy field soil under rice cultivation (1993)

Proteolytic bacteria in paddy field soils under rice cultivation were characterized and enumerated using azocoll agar plates. Bacillus spp. were the proteolytic bacteria that were most frequently present, comprising 59% of the isolates. They were always the numerically dominant proteolytic bacteria isolated from three kinds of fertilizer treatments (yearly application of rice-straw compost and chemical fertilizer, yearly application of chemical fertilizer, and no fertilizer application) and at three different stages of rice development (vegetative growth stage, maximal tillering stage, and harvest stage). Of the 411 proteolytic bacteria isolated, 124 isolates had stronger proteolytic activity than others on the basis of gelatin liquefaction tests and most of them were Bacillus spp. (100% in 1989 and 92.4% in 1991). Bacillus subtilis and Bacillus cereus were the main bacteria of this group and Bacillus mycoides, Bacillus licheniformis, and Bacillus megaterium were also present. We conclude that these Bacillus spp. are the primary source of soil protease in these paddy fields.
The application of FAIMS gas analysis in medical diagnostics (2015)

There is an ever increasing need to develop new tools to aid in the diagnosis and monitoring of human diseases. Such tools will ultimately reduce the cost of healthcare by identifying disease states more quickly and cheaply than current practices. One method showing promise is the analysis of gas-phase biomarkers from human breath, urine, sweat and stool that reflect bodily metabolism. Analysis of these volatiles by GC MS requires specialised infra-structure and staff, making it unsuitable for a clinical setting. Point of care sensor based technologies such as eNoses often suffer from stability and sensitivity issues. Field-Asymmetric Ion Mobility Spectrometry (FAIMS) has potential to fulfil this clinical need. In this paper we review the medical need, the technology, sampling methods and medical evidence thus far. We conclude with reflecting on future developmental steps necessary to bring the device into medical practice.
A Systematic Comparison of 18F-C-SNAT to Established Radiotracer Imaging Agents for the Detection of Tumor Response to Treatment (2015)

We have demonstrated here that (18)F-C-SNAT can sensitively detect drug-induced cell death in murine lymphoma and human NSCLC. Despite favorable image contrast obtained with (18)F-C-SNAT, the development of next-generation derivatives, using the same novel and promising uptake mechanism, but displaying improved biodistribution profiles, are warranted for maximum clinical utility.
Persistent Cryptosporidium infection in congenitally athymic (nude) mice (1984)

Nude (nu/nu) BALB/c mice and their white (nu/+) littermates were experimentally infected with Cryptosporidium sp. at 6 days of age. In white mice, the infection was transient, but in nude mice a persistent infection developed that was characterized by diarrhea and, occasionally, death. There were villus atrophy and crypt hyperplasia in the small intestine of infected nude and white mice necropsied at 11 days of age. Persistently infected nude mice had, in addition to the above small intestinal lesions, diffuse cystic mucosal hyperplasia and crypt abscesses in the large intestine at 56 days of age. These results suggest that T cells are required for recovery from the Cryptosporidium infection but are not required for epithelial cell loss in cryptosporidiosis. Both nude and white mice appeared to be relatively more resistant to Cryptosporidium infection at 42 days of age than at 6 days of age.
Perceptual media compression for multiple viewers with feedback delay (2005)

Human eyes have limited perception capabilities; for example, only 2 degrees of our 140 degree vision field provide the highest quality of perception. Due to this fact the idea of perceptual focus emerged to allow a visual content to be changed in a way that only part of the visual field where a human gaze is directed is encoded with a high quality. The image quality in the periphery can be reduced without a viewer noticing it. This compression approach allows a significant decrease in the number of bits required for image encoding, and in the case of the 3D image rendering, it decreases the computational burden. A number of previous researchers have investigated the topic of perceptual focus but only for a single viewer. In our research we investigate a dynamically changing multi-viewer scenario. In this type of scenario a number of people are watching the same visual content at the same time. Each person has his/her own perceptual focus area which changes over time. The visual content is sent through a network with a fixed delay/lag which provides an additional challenge to the whole scheme. The goal of our work was to investigate and develop a method of multi-viewer perceptual focus zones adaptation for real-time media perceptual compression and transmission. In our research we also look into the impact that such a method can have on transmission bandwidth and computational burden reduction.
Anti-Candida activity of polyoxin: example of peptide transport in yeasts (1984)

The polyoxins, nucleoside peptide antibiotics, are effective inhibitors of chitin synthesis in some fungi and yeasts. Although isolated chitin synthetases appear to be equally sensitive to inhibition by polyoxins, intact yeast cells are relatively insensitive. It has been suggested that polyoxins enter cells by a peptide carrier transport mechanism. In this paper, we report results which demonstrate that changes in the growth conditions significantly affect the degree of sensitivity of Canidida albicans to the polyoxins. For example, a minimal inhibitory concentration of 0.1 micrograms/ml was obtained in a defined medium compared with a minimal inhibitory concentration of 1,000 micrograms/ml with conventional media. Various noninhibitory di- and tripeptides, when added to the media, were found to antagonize the anti-Candida activity of polyoxins. In addition, polyoxin-resistant mutants of C. albicans were shown to exhibit cross-resistance with other dipeptide antibiotics. The data reported herein support peptide transport of polyoxins in C. albicans.
Synthesis and properties of 12-fluororetinal and 12-fluororhodopsin. Model system for fluorine-19 NMR studies of visual pigments (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and properties of 12-fluororetinal and 12-fluororhodopsin. Model system for fluorine-19 NMR studies of visual pigmentsRobert S. H. Liu, Hiroyuki Matsumoto, Alfred E. Asato, Marlene Denny, Yoshinori Shichida, Toru Yoshizawa, and F. W. DahlquistCite this: J. Am. Chem. Soc. 1981, 103, 24, 7195–7201Publication Date (Print):December 1, 1981Publication History Published online1 May 2002Published inissue 1 December 1981https://doi.org/10.1021/ja00414a026RIGHTS & PERMISSIONSArticle Views212Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (724 KB) Get e-Alertsclose Get e-Alerts