gebhart/patrepeval huggingface dataset:
THE ORIGIN OF SERUM PROTEIN, A, B AND H BLOOD GROUP, AND Gm AND Inv ANTIGENS IN HOUSE DUST (1976)

Materials precipitated from an aqueous extract of house dust by saturation with ammonium sulphate showed immunological reaction with antisera to human serum albumin, human alpha1-acid glycoprotein, human IgG, Gm and Inv antigens and to A, B and H antigens. It is concluded that the albumin and alpha1-acid glycoprotein are of human origin. It seems that the apparent IgG activity is due to cross-reactivity since, from the anticipated specificities of the Fab and Fc fragments of human IgG, the former could not be detected, and since Gm (6) activity was present, unexpectedly, in Swiss house dust. The A, B and H blood group antigenic activities were detected in relative concentration different from those expected if they were solely of human origin. It is concluded that they are from both human and non-human sources.
Disease patterns and tissue cytokine profiles in giant cell arteritis (1997)

Variations in the clinical presentation of GCA were correlated with cytokine mRNA expression in the affected temporal arteries. Differences in the effector functions of tissue-infiltrating T cells distinguished disease patterns in which either local ischemic symptoms or systemic involvement was dominant, or in which there was co-occurrence of PMR. Definition of different patterns of inflammation in GCA might, therefore, facilitate the design of differentiated therapeutic approaches.
Facilitating polymer conjugation via combination of RAFT polymerization and activated ester chemistry (2010)

Abstract The synthesis of block copolymers via polymer conjugation of well‐defined building blocks offers excellent control over the structures obtained, but often several coupling strategies need to be explored to find an efficient one depending on the building blocks. To facilitate the synthesis of polymers with adjustable functional end‐groups for polymer conjugation, we report on the combination of activated ester chemistry with RAFT polymerization using a chain transfer agent (CTA) with a pentafluorophenyl ester (PFP‐CTA), which allows for flexible functionalization of either the CTA prior to polymerization or the obtained polymer after polymerization. Different polymethacrylates, namely PMMA, P( t ‐BuMA) and PDEGMEMA, were synthesized with an alkyne‐CTA obtained from the aminolysis of the PFP‐CTA with propargylamine, and the successful incorporation of the alkyne moiety could be shown via 1 H and 13 C NMR spectroscopy and MALDI TOF MS. Further, the reactive α‐end‐groups of polymers synthesized using the unmodified PFP‐CTA could be converted into azide and alkyne end‐groups after polymerization, and the high functionalization efficiencies could be demonstrated via successful coupling of the resulting polymers via CuAAC. Thus, the PFP‐CTA allows for high combinatory flexibility in polymer synthesis facilitating polymer conjugation as useful method for the synthesis of block copolymers. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
Mesenchymal stem cells generate a CD4+CD25+Foxp3+ regulatory T cell population during the differentiation process of Th1 and Th17 cells (2013)

This study demonstrated that MSCs contribute to the generation of an immunosuppressive environment via the inhibition of proinflammatory T cells and the induction of T cells with a regulatory phenotype. Together, these results might have important clinical implications for inflammatory and autoimmune diseases.
Molecular studies of human genetic disease (1991)

A wide variety of techniques are available for detecting disease-causing mutations within human genes; this report provides a brief review of such procedures. Good communication and exchange of materials between the clinical genetics field and the Human Genome Initiative will benefit both.
Cellular proliferation, estrogen receptor, progesterone receptor, and bcl-2 expression in GnRH agonist-treated uterine leiomyomas (1998)

Gonadotropin-releasing hormone (GnRH) agonists are commonly used in the treatment of uterine leiomyomas, but little is known about their histological and cellular effects on these neoplasms. We examined a cellular proliferation index as determined by the nuclear antigen Ki-67 and proliferating cell nuclear antigen (PCNA) expression, estrogen receptor (ER), and progesterone receptor (PR) expression in 27 leiomyomas from patients treated with the GnRH agonist leuprolide acetate (LA) and compared them with 33 untreated controls. All leiomyomas were removed by myomectomies from premenopausal woman after 2 to 6 months of LA treatment or in the follicular phase of the menstrual cycle in the untreated controls. Histological features examined included cellularity, nuclear atypia, vascular changes (dilated, thickened, or thrombosed vessels), edema, calcification, hemorrhage, necrosis, hyalinization, and mitotic activity. Although no difference was found between GnRH-treated and nontreated groups with respect to most histological features examined, immunohistochemical studies showed a significant decrease in the cellular proliferation index, ER, and PR expression in the LA-treated cases compared with nontreated controls. The cellular proliferation index, ER, and PR expression decreased by 85%, 49%, and 36%, respectively, in the LA-treated group as compared with controls (P < .001). A subset of cases from the LA-treated and nontreated groups were also analyzed with respect to bcl-2 (an inhibitor of apoptosis) expression, and no significant difference between the LA-treated and nontreated groups was observed with both groups showing a strong (> 75% of cells) cytoplasmic staining pattern. Results of this study show that LA treatment of leiomyomas results in a decrease in number of cycling cells.
Frequency-resolved optical gating using cascaded second-order nonlinearities (1998)

We demonstrate frequency-resolved optical gating (FROG) using cascaded second-order nonlinearities (up-conversion followed by down-conversion). We describe two different cascaded second-order beam geometries-self-diffraction and polarization-gate-which are identical to their third-order nonlinear-optical cousins, except that they use second-harmonic-generation crystals instead of (weaker) third-order materials. Like the corresponding third-order processes, these new versions of FROG yield the same intuitive traces, uniquely determine the pulse intensity and phase (without direction-of-time ambiguity), and yield signal light at the input-pulse wavelength (which simplifies the required spectral measurements). Most importantly, however, we show that these techniques are significantly more sensitive than the corresponding third-order FROG methods, conveniently allowing, for the first time, the unambiguous measurement of ultrashort /spl sim/1-nJ pulses, that is, unamplified Ti:sapphire oscillator pulses.
Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation (2004)

BACKGROUND: Antibody fragments are molecules widely used for diagnosis and therapy. A large amount of protein is frequently required for such applications. New approaches using folding reporter enzymes have recently been proposed to increase soluble expression of foreign proteins in Escherichia coli. To date, these methods have only been used to screen for proteins with better folding properties but have never been used to select from a large library of mutants. In this paper we apply one of these methods to select mutations that increase the soluble expression of two antibody fragments in the cytoplasm of E. coli. RESULTS: We used the beta-galactosidase alpha-complementation system to monitor and evolve two antibody fragments for high expression levels in E. coli cytoplasm. After four rounds of mutagenesis and selection from large library repertoires (>107 clones), clones exhibiting high levels of beta-galactosidase activity were isolated. These clones expressed a higher amount of soluble fusion protein than the wild type in the cytoplasm, particularly in a strain deficient in the cytoplasmic Lon protease. The increase in the soluble expression level of the unfused scFv was, however, much less pronounced, and the unfused proteins proved to be more aggregation prone than the wild type. In addition, the soluble expression levels were not correlated with the beta-galactosidase activity present in the cells. CONCLUSION: This is the first report of a selection for soluble protein expression using a fusion reporter method. Contrary to anticipated results, high enzymatic activity did not correlate with the soluble protein expression level. This was presumably due to free alpha-peptide released from the protein fusion by the host proteases. This means that the alpha-complementation assay does not sense the fusion expression level, as hypothesized, but rather the amount of free released alpha-peptide. Thus, the system does not select, in our case, for higher soluble protein expression level but rather for higher protease susceptibility of the fusion protein.
Visualization of Transepithelial Passage of the Immunogenic 33-Residue Peptide from α-2 Gliadin in Gluten-Sensitive Macaques (2010)

The rhesus macaque model of gluten sensitivity not only resembles the histopathology of CD but it also may provide a model for studying intestinal permeability in states of epithelial integrity and disrepair.
The Estimation of Field-Dependent Conductance Change of Nanopore by Field-Induced Charge in the Translocations of AuNPs-DNA Conjugates (2014)

Solid-state nanopores have been proven to be a powerful tool for the characterization of individual molecules and nanoparticles. The basic motivation of this technique is to determine the particle size by the conductance change during the translocation of the particle. However, there still has not been a quantitative estimation of the dependence of electric field on the conductance change due to a particle translocation. Here, we present the first observations of the intriguing biphasic and asymmetrical events in the translocations of DNA-modified gold nanoparticles through ∼60 nm nanopores. An electric field-dependent conductance change and quadratic nonlinear electrophoresis were observed as well. Thus, we develop an approximation of the conductance change of nanopore based on induced-charge electrophoresis. The effects of salt concentration, the applied voltage, and particle radius on the conductance change are studied. This study gives a fundamental understanding and provides valuable suggestions to understand the translocation of biomolecular attached metal nanoparticles through nanopores. The results indicate a novel way for direct observation and study of nonlinear electrophoresis of single nanoparticles using nanopore technique as well.
Genomic organization of mouse Fc gamma receptor genes. (1990)

We have isolated and characterized the gene coding for the mouse Fc receptor that is termed Fc gamma RIIa. The gene contains five exons and spans approximately 9 kilobases. Unlike most members of the immunoglobulin gene superfamily, this gene utilizes multiple exons to encode its leader peptide. The first exon encodes the hydrophobic region of the signal sequence; the second exon, which contains only 21 base pairs, encodes a segment of the signal peptidase recognition site; and the beginning of the third exon encodes the predicted site of peptidase cleavage. The third and fourth exons each code for immunoglobulin-like extracellular domains. The fifth exon encodes the hydrophobic transmembrane domain and the cytoplasmic tail. Partial characterization of the Fc gamma RIIb gene indicates that it also contains multiple leader exons, including a 21-base-pair exon and two exons coding for homologous immunoglobulin-like extracellular domains. However, the Fc gamma RIIb gene uses four exons to encode its intracytoplasmic region. Analysis using contour-clamped homogeneous electric field (CHEF) gels indicates that the Fc gamma RIIa and Fc gamma RIIb genes are linked within 160 kilobases on mouse chromosome 1.
Clinical Trials of Amniotic Membranes in Burn Wound Care (1982)

Four test conditions of increasing complexity were used to evaluate the clinical efficacy of amniotic membranes as biologic dressings on donor sites and burn wounds in children. These were the clean-skin donor-site wound, the uncontaminated shallow partial-thickness burn wound, the bed of freshly excised full-thickness wounds, and the granulating surface of colonized burn wounds. The rate of epithelialization under amniotic membranes was the same as that under 5% scarlet red ointment or 0.5% silver nitrate solution dressings. Preservation of a healthy excised wound bed and maintenance of a low bacterial count in contaminated wounds paralleled the experience with human allograft dressings despite technical difficulties and the absence of vascularization of amniotic membrane and its fragile structure. Tentative conclusions are drawn as to the mechanisms by which biologic dressings exert their beneficial effects.
Requirement for CD8 + Cells in T Cell Receptor Peptide-Induced Clonal Unresponsiveness (1993)

T cell receptor (TCR) vaccination in rats prevents the development of experimental allergic encephalomyelitis (EAE), an animal model of multiple sclerosis. The mechanism of this potential immunotherapy was examined by vaccinating mice with an immunogenic peptide fragment of the variable region of the TCR V beta 8.2 gene. Another immunogen that usually induces an immune response mediated by V beta 8.2+ T cells was subsequently inhibited because specific clonal unresponsiveness (anergy) had been induced. Depletion of CD8+ cells before TCR peptide vaccination blocked such inhibition. Thus, the clonal anergy was dependent on CD8+ T cells, and such immunoregulatory T cells may participate in the normal course of EAE.
Eukaryotic DNA Polymerases (2002)

Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during cell division, DNA repair, DNA recombination, and the bypassing of damage in DNA. DNA polymerases (pols) alpha, beta, gamma, delta, and epsilon are the key enzymes required to maintain the integrity of the genome under all these circumstances. In the last few years the number of known pols, including terminal transferase and telomerase, has increased to at least 19. A particular pol might have more than one functional task in a cell and a particular DNA synthetic event may require more than one pol, which suggests that nature has provided various safety mechanisms. This multi-functional feature is especially valid for the variety of novel pols identified in the last three years. These are the lesion-replicating enzymes pol zeta, pol eta, pol iota, pol kappa, and Rev1, and a group of pols called pol theta;, pol lambda, pol micro, pol sigma, and pol phi that fulfill a variety of other tasks.
What Is the Role of Cytotoxic T Lymphocyte–Associated Antigen 4 Blockade in Patients with Metastatic Melanoma? (2009)

With increasing knowledge of the molecular basis of the immune system and mechanisms of tumor tolerance, novel approaches to treating malignant diseases refractory to standard therapies are being investigated. Monoclonal antibodies (mAbs) that bind cytotoxic T lymphocyte-associated antigen (CTLA)-4 can block inhibitory signals normally generated through this receptor, thus prolonging and sustaining T-cell activation and proliferation. These antibodies are being developed and tested in patients with metastatic melanoma. This article reviews data published or presented at scientific congresses describing the clinical safety and antitumor activity of two different anti-CTLA-4 mAbs: tremelimumab (CP-675,206) and ipilimumab (MDX-010). Overall, although the response rate has not been consistently higher than the response rates associated with other treatments, the induction of durable responses and the favorable safety profile observed with anti-CTLA-4 mAbs are encouraging. However, the true advantage of these new drugs may depend largely on the characterization of predictive biomarkers of activity and subsequent targeting of responsive patients.
Hybrid wireless-broadband over power lines: A promising broadband solution in rural areas (2009)

In rural areas, due to the low population density and the poor familiarization with new technologies, new projects related to broadband access are less profitable. In this article a hybrid wireless-broadband over power lines network, suitable for rural and remote areas is presented, offering smart grid applications and broadband access along a 107 km medium voltage power grid in Larissa, a rural area in central Greece. This hybrid solution exploits the ubiquitous presence of the power grid along with the adaptability and accessibility of wireless technology. Through the design and implementation of this hybrid network many aspects of BPL technology were successfully dealt with demonstrating that, in addition to enhancing the power grid performance, W-BPL technology may be considered as an alternative broadband solution in rural and/or sparsely populated areas.
Built-in via module test structure for backend interconnection in-line process monitor (2005)

The best in-line process monitoring test structure should have multiple purposes, one of which is to allow easy assessing and quick defect identification. In this study, the via module test structure has been designed allowing easy assessment of the via profiles of via, coupled with the resistance measurement of the via, such can be used as effect via module defect monitor. This structure can be used as frame monitor structure. Via module test structure is realized in Cu/low K (Black Diamond /spl trade/) interconnection.
Ability of the hydrophobic FGF and basic TAT peptides to promote cellular uptake of recombinant Cre recombinase: A tool for efficient genetic engineering of mammalian genomes (2002)

Conditional mutagenesis is a powerful tool to analyze gene functions in mammalian cells. The site-specific recombinase Cre can be used to recombine loxP-modified alleles under temporal and spatial control. However, the efficient delivery of biologically active Cre recombinase to living cells represents a limiting factor. In this study we compared the potential of a hydrophobic peptide modified from Kaposi fibroblast growth factor with a basic peptide derived from HIV-TAT to promote cellular uptake of recombinant Cre. We present the production and characterization of a Cre protein that enters mammalian cells and subsequently performs recombination with high efficiency in a time- and concentration-dependent manner. Histidine-tagged Cre recombinase transduced inefficiently unless fused to a nuclear localization signal (NLS). Fusion of NLS-Cre to the fibroblast growth factor transduction peptide did not improve the transducibility, whereas fusion with the TAT peptide significantly enhanced cellular uptake and subsequent recombination. More than 95% recombination efficiency in fibroblast cells, as well as murine embryonic stem cells, was achieved after His-TAT-NLS-Cre transduction. Efficient recombination could also be obtained in primary splenocytes ex vivo. We expect that application of His-TAT-NLS-Cre, which can be produced readily in large quantities from a bacterial source, will expand our abilities to manipulate mammalian genomes.
Defining the extracellular matrix using proteomics (2013)

The cell microenvironment has a profound influence on the behaviour, growth and survival of cells. The extracellular matrix (ECM) provides not only mechanical and structural support to cells and tissues but also binds soluble ligands and transmembrane receptors to provide spatial coordination of signalling processes. The ability of cells to sense the chemical, mechanical and topographical features of the ECM enables them to integrate complex, multiparametric information into a coherent response to the surrounding microenvironment. Consequently, dysregulation or mutation of ECM components results in a broad range of pathological conditions. Characterization of the composition of ECM derived from various cells has begun to reveal insights into ECM structure and function, and mechanisms of disease. Proteomic methodologies permit the global analysis of subcellular systems, but extracellular and transmembrane proteins present analytical difficulties to proteomic strategies owing to the particular biochemical properties of these molecules. Here, we review advances in proteomic approaches that have been applied to furthering our understanding of the ECM microenvironment. We survey recent studies that have addressed challenges in the analysis of ECM and discuss major outcomes in the context of health and disease. In addition, we summarize efforts to progress towards a systems-level understanding of ECM biology.
Supersmall flying optical head for phase change recording media (1989)

A supersmall optically switched laser (OSL) head is proposed. A laser diode attached to an air bearing slider forms a complex cavity together with a recording medium, and its light output is detected by a photodetector placed at the other end of the laser facet. Data signals and track error signals read from the sampled servo marks are successfully detected. The signal amplitude variation caused by the flying height change is much reduced, and the SNR is increased to 36 dB (40 kHz to 20 MHz for a phase change medium) by decreasing the reflectivity of the laser facet facing the medium to <5%.
Discrete-time variance tracking with application to speech processing (2001)

Two new discrete-time algorithms are presented for tracking variance and reciprocal variance. The closed&#13;\nloop nature of the solutions to these problems makes this approach highly accurate and can be used&#13;\nrecursively in real time. Since the Least-Mean Squares (LMS) method of parameter estimation requires an&#13;\nestimate of variance to compute the step size, this technique is well suited to applications such as speech&#13;\nprocessing and adaptive filtering.
Financial and Property (2009)

Financial and property orders include: transfer or sale of land and other assets; payment of moneys; who is to retain motor vehicles, furniture, etc; superannuation orders; and spousal maintenance.
Oral desensitizing treatment in food allergy: clinical and immunological results (2003)

The immunological findings induced by oral desensitization in food allergy allow us to hypothesize that oral tolerance may be mediated by the same mechanisms as those involved in traditional desensitizing treatments for respiratory allergies. Moreover, the proposed standardized oral desensitization protocols may represent an effective alternative approach in the management of food-allergic patients.