gebhart/patrepeval huggingface dataset:
Role of Natural Killer Cell Subsets in Cardiac Allograft Rejection (2006)

To achieve donor-specific immune tolerance to allogeneic organ transplants, it is imperative to understand the cell types involved in acute allograft rejection. In wild-type mice, CD4(+) T cells are necessary and sufficient for acute rejection of cardiac allografts. However, when T-cell responses are suboptimal, such as in mice treated with costimulation-targeting agents or in CD28-deficient mice, and perhaps in transplanted patients taking immunosuppressive drugs, the participation of other lymphocytes such as CD8(+) T cells and NK1.1(+) cells becomes apparent. We found that host NK but not NKT cells were required for cardiac rejection. Ly49G2(+) NK cells suppressed rejection, whereas a subset of NK cells lacking inhibitory Ly49 receptors for donor MHC class I molecules was sufficient to promote rejection. Notably, rejection was independent of the activating receptors Ly49D and NKG2D. Finally, our experiments supported a mechanism by which NK cells promote expansion and effector function of alloreactive T cells. Thus, therapies aimed at specific subsets of NK cells may facilitate transplantation tolerance in settings of impaired T-cell function.
N-alkylated oligoamide α-helical proteomimetics (2010)

Generic approaches for the design and synthesis of small molecule inhibitors of protein-protein interactions (PPIs) represent a key objective in modern chemical biology. Within this context, the alpha-helix mediated PPIs have received considerable attention as targets for inhibition using small molecules, foldamers and proteomimetics. This manuscript describes a novel N-alkylated aromatic oligoamide proteomimetic scaffold and its solid-phase synthesis--the first time such an approach has been used for proteomimetics. The utility of these scaffolds as proteomimetics is exemplified through the identification of potent microM inhibitors of the p53-hDM2 helix mediated PPI--a key oncogenic target.
Estimating the prevalence of pyruvate kinase deficiency from the gene frequency in the general white population (2000)

Pyruvate kinase (PK) deficiency is the most common cause of hereditary nonspherocytic hemolytic anemia. The prevalence of this deficiency is unknown, though some estimates have been made based on the frequency of low red cell PK activity in the population. An additional 20 patients with hereditary nonspherocytic hemolytic anemia caused by PK deficiency have been genotyped. One previously unreported mutation 1153C-->T (R385W) was encountered. The relative frequency of PK mutations in patients with hemolytic anemia caused by PK deficiency was calculated from the 18 white patients reported here and from 102 patients previously reported in the literature. DNA samples from 3785 subjects from different ethnic groups have been screened for the 4 more frequently encountered mutations-c.1456 C-->T(1456T), c.1468 C-->T(1468T), c.1484 C-->T(1484T), and c.1529 G6A (1529A)-by allele-specific oligonucleotide hybridization. Among white patients the frequency of the 1456T mutation was 3.50 x 10(-3); that of the 1529A mutation was 2.03 x 10(-3). Among African Americans the frequency of the 1456T mutation was 3.90 x 10(-3) The only mutation found in the limited number of Asians tested was 1468T at a frequency of 7.94 x 10(-3). Based on the gene frequency of the 1529A mutation in the white population and on its relative abundance in patients with hemolytic anemia caused by PK deficiency, the prevalence of PK deficiency is estimated at 51 cases per million white population. This number would be increased by inbreeding and decreased by failure of patients with PK deficiency to survive.
Visualizing large trees using the hyperbolic browser (1996)

We demonstrate a focus+context (fisheye) scheme for viswdizing and manipulating kuge hierarchies. Our approach is to lay out the hierarchy uniformly on the hyperbolic plane and map this plane onto a circular display region. 'l'he projection onto the disk provides a natural mechanism for assigning more space to a portion of the hierarchy while still embedding it in a much larger context. Change of focus is accomplished by tmnslatingthe structure on the hyperbolic plane, which allows a smooth transition without compromising the presentation of the context.
Effect of Molecular Crowding on the Response of an Electrochemical DNA Sensor (2007)

E-DNA sensors, the electrochemical equivalent of molecular beacons, appear to be a promising means of detecting oligonucleotides. E-DNA sensors are comprised of a redox-modified (here, methylene blue or ferrocene) DNA stem-loop covalently attached to an interrogating electrode. Because E-DNA signaling arises due to binding-induced changes in the conformation of the stem-loop probe, it is likely sensitive to the nature of the molecular packing on the electrode surface. Here we detail the effects of probe density, target length, and other aspects of molecular crowding on the signaling properties, specificity, and response time of a model E-DNA sensor. We find that the highest signal suppression is obtained at the highest probe densities investigated, and that greater suppression is observed with longer and bulkier targets. In contrast, sensor equilibration time slows monotonically with increasing probe density, and the specificity of hybridization is not significantly affected. In addition to providing insight into the optimization of electrochemical DNA sensors, these results suggest that E-DNA signaling arises due to hybridization-linked changes in the rate, and thus efficiency, with which the redox moiety collides with the electrode and transfers electrons.
The Business Models of E-Marketplace (2015)

An electronic marketplace (EM) is an inter-organizational information intermediary that enables participating buyers and sellers to exchange information about price and product offerings and to cooperate on commodity exchange. Prior researches on EM design have not defined EM business model clearly. With a review of existing literatures on business model, this paper developed taxonomy of EM business models and classified existing EMs business model from a network role perspective. Further discussion of the evolution of EM business model is provided at the end.
Oncolytic enteroviruses (2012)

Increasing information concerning molecular biology of viruses and virus-cell interactions makes it possible to use viruses as a tool in effort to treat cancer diseases. As a rule, tumor cells are highly sensitive to viruses that may be used in cancer therapy. Therewith, applications of viral oncolysis in treatment of cancer diseases assume maximum possible safety of used viruses for patient and environment. Human enteroviruses are one of the most convenient sources to generate oncolytic viruses. Many of enteroviruses are non-pathogenic for humans or cause mild disease. Progress in genetic engineering permits to develop attenuated enterovirus variants with high safety and selectivity. This review focuses on the main members of Enterovirus genus, such as Coxsackieviruses, and vaccine strains as promising source for development of oncolytic agents, applicable for cancer therapy. It reviews data concerning recently developed and tested oncolytic variants of enteroviruses and discusses perspectives of their application in cancer therapy and problems, concerning their improvement and practical use.
Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli. (1981)

A herpes simplex virus type I DNA fragment containing the sequence coding for thymidine kinase was fused to the very beginning of the Escherichia coli lac Z gene in the three possible reading frames. When the thymidine kinase sequence was in the orientation fit to be transcribed from the lac promoter, functional thymidine kinase was made under lac control in all three cases. Sequences data indicate that translation reinitiation occurs at the 5' end of the thymidine kinase gene after stop signals. Two T+A-rich sequences, which may be part of eukaryotic promoters, are found in the same region.
Formation of acetamido‐Aza‐steroids (1990)

Abstract Beckmann rearrangement of 3α‐ and 3β‐acetamido‐5α‐androstan‐17‐one oximes gives homo‐aza compounds and the corresponding “second order” Beckmann cleavage ω‐cyanoolefin. The mixture of the compounds produced, was separated by column chromatography. The structure of the lactams and the exocyclic nitriles was apparent from ir and nmr spectra.
Theory of the Impedance of Electron Diffusion and Recombination in a Thin Layer (2001)

This paper analyzes the small signal ac impedance of electron diffusion and recombination in a spatially restricted situation with application in systems such as porous TiO2 nanostructured photoelectrodes and intrinsically conducting polymers. It is shown that the diffusion−recombination model with the main types of boundary conditions assumes a finite set of possible behaviors in the frequency domain, which are classified according to relevant physical parameters. There are four possible cases: (i) the impedance of finite diffusion with reflecting boundary, (ii) the impedance of finite diffusion with absorbing boundary, (iii) the impedance of diffusion-reaction in semiinfinite space or Gerischer impedance, and (iv) the impedance that combines Warburg response at high frequency and a reaction arc at low frequency. The generality of the approach is discussed in terms of the spatial distribution of the electrochemical potential or quasi-Fermi level and also in terms of the transmission line representation. An extension is considered to the diffusion in lithium intercalation electrodes coupled to a homogeneous solid-state reaction. The connection is established with other frameworks for the description of transport and reaction in electrochemical systems.
Recurring loss involving chromosomes 1, 3, and 22 in malignant mesothelioma: Possible sites of tumor suppressor genes (1989)

Cytogenetic analysis was performed on short-term cultures of primary tumor tissue obtained from five patients with pleural malignant mesothelioma. Clonal karyotypic abnormalities were detected in four patients, none of whom had received cytotoxic therapy prior to karyotypic evaluation. Recurring chromosomal changes included partial deletions of 1p and 3p, and monosomy of 18, 19, and 22. We also reviewed data on 24 previously reported pretreatment patients and determined that alterations of chromosomes 1, 3, and 22 are frequently associated with malignant mesothelioma. Partial loss of chromosome 1 due to deletions or other rearrangements most frequently involve bands 1p11-pter with the shortest region of overlap (SRO) occurring at 1p21-p22 in our patients. Deletions and other structural changes of chromosome 3 usually involve the region 3p14-p25. The SRO of 3p deletions appeared to be at band 3p21. Monosomy 22 represents the most consistent specific whole chromosome loss seen in malignant mesothelioma, being observed in 11 of 28 cases summarized. In addition, structural changes of 22q have been observed in three patients, and a breakpoint at 22q11 was reported in each case. Taken collectively, these data suggest that a cascade of events involving alterations of genes on more than one specific chromosome may play a critical role in the development of malignant mesothelioma. The pattern of recurring chromosomal loss, particularly of 1p, 3p, and 22q, indicates that these regions should be targeted for future molecular investigations into the possible involvement of suppressor genes in this malignancy.
Fracture of first rib after sternotomy (2011)

Sir, Rib fracture is a known complication following median sternotomy mainly attributed to excessive retraction.[12] The symptoms depend upon the location of the fracture and associated brachial plexus injury. Sometimes symptoms persist because of occult rib fracture requiring special investigations like bone scan.[3] A 71-year-old gentleman with coronary artery and ischemic mitral regurgitation presented for coronary artery bypass grafting (CABG) and mitral valve repair. He was suffering from hypertension and chronic renal disease. His preoperative investigations revealed serum creatinine of 1.8 mg/dl and blood urea of 109 mg/dl. The rest of the investigations were within normal limits. Chest radiograph revealed cardiomegaly and congested lung fields [Figure 1]. Echocardiography showed global hypokinesia of the left ventricle with ejection fraction of 30% and moderate to severe mitral regurgitation with predicted pulmonary artery pressure (PAP) of 50 mmHg. He underwent CABG with three saphenous vein grafts and mitral valve repair with Carpentier Edwards classic ring. Total cardiopulmonary bypass (CPB) and aortic cross clamp time was 161 and 108 min respectively. Total surgical time was 350 min. Sternal retraction was done with double-blade retractor. Intraoperative period was uneventful. During the postoperative period, the patient was haemodynamically stable with minimal inotropic support. Postoperative portable chest radiograph revealed fracture of the left first rib [Figure 2]. Patient was electively ventilated for 16 h postoperatively in view of high PAP and combined surgical procedure. Patient did not have pain, numbness or parasthesia in the left upper limb. There was no evidence suggestive of associated brachial plexus injury.Figure 1: Preoperative chest radiographFigure 2: Postoperative chest radiograph showing fracture of left first ribRib fracture is common after sternotomy and often goes unnoticed. The symptoms depend on location of the fracture. One-third of the fractures are reported each in the first and second rib and the remaining third throughout the thorax.[3] Occult rib fractures are often a major cause of non-incisional chest pain in patients who have undergone sternotomy.
The ratio between CcdA and CcdB modulates the transcriptional repression of the ccd poison–antidote system (2001)

The ccd operon of the F plasmid encodes CcdB, a toxin targeting the essential gyrase of Escherichia coli, and CcdA, the unstable antidote that interacts with CcdB to neutralize its toxicity. Although work from our group and others has established that CcdA and CcdB are required for transcriptional repression of the operon, the underlying mechanism remains unclear. The results presented here indicate that, although CcdA is the DNA-binding element of the CcdA-CcdB complex, the stoichiometry of the two proteins determines whether or not the complex binds to the ccd operator-promoter region. Using electrophoretic mobility shift assays, we show that a (CcdA)2-(CcdB)2 complex binds DNA. The addition of extra CcdB to that protein-DNA complex completely abolishes DNA retardation. Based on these results, we propose a model in which the ratio between CcdA and CcdB regulates the repression state of the ccd operon. When the level of CcdA is superior or equal to that of CcdB, repression results. In contrast, derepression occurs when CcdB is in excess of CcdA. By ensuring an antidote-toxin ratio greater than one, this mechanism could prevent the harmful effect of CcdB in plasmid-containing bacteria.
Rancidity and its measurement in edible oils and snack foods. A review (1988)

K. Robards, A. F. Kerr and E. Patsalides, Analyst, 1988, 113, 213 DOI: 10.1039/AN9881300213
Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat Germ (1973)

Extracts prepared from commercially available wheat germ efficiently translate messenger RNAs from either viral or eukaryotic origin. The addition of tobacco mosaic virus RNA stimulated amino-acid incorporation more than 100-times and rabbit globin 9S RNA between 20- and 30-times. The in vitro product directed by globin 9S RNA comigrated precisely with authentic rabbit globin on sodium dodecyl sulfate-polyacrylamide gels. Furthermore, during high voltage ionophoresis two [(35)S]methionine-labeled tryptic peptides synthesized in vitro comigrated in two dimensions with alphaT5 and betaT5 tryptic peptides from authentic [(35)S]methionine-labeled rabbit globin.
Testing 144- and 256-crystal BGO block detectors (1994)

New block detectors have been fabricated incorporating large numbers of small crystals. We evaluate and compare the performance of the new detectors with a standard 64-crystal block detector from Siemans-CTI. The new detectors demonstrate greatly improved imaging capability for 511 keV gamma rays. Future PET tomographs incorporating such detectors should produce substantially better volume images with little increase in tomograph manufacturing costs. The detectors will require a new type of automatic calibration procedure. Various such procedures have been tested and are discussed. A technique using higher energy gamma rays has shown special promise.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Traumatic Brain Injury and Delayed Sequelae: A Review - Traumatic Brain Injury and Mild Traumatic Brain Injury (Concussion) are Precursors to Later-Onset Brain Disorders, Including Early-Onset Dementia (2007)

Brain injuries are too common. Most people are unaware of the incidence of and horrendous consequences of traumatic brain injury (TBI) and mild traumatic brain injury (MTBI). Research and the advent of sophisticated imaging have led to progression in the understanding of brain pathophysiology following TBI. Seminal evidence from animal and human experiments demonstrate links between TBI and the subsequent onset of premature, psychiatric syndromes and neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD). Objectives of this summary are, therefore, to instill appreciation regarding the importance of brain injury prevention, diagnosis, and treatment, and to increase awareness regarding the long-term delayed consequences following TBI.
Search for Potential Antiviral Drugs. Part I. Synthesis of Substituted Butyrophenone Thiosemicarbazones (1974)

Department of Chemistry, University of Lucknow, Lucknow. <em>Manuscript received 3 September 1973; revised 11 April 974; accepted 23 May 1974</em> Search for Potential Antiviral Drugs. Part I. Synthesis of Substituted Butyrophenone Thiosemicarbazones
Cross‐presentation of epitopes on virus‐like particles via the MHC I receptor recycling pathway (2011)

Effective vaccines and immunotherapies against cancer require professional antigen-presenting cells to cross-present exogenous antigen to initiate cytotoxic T-cell responses to destroy tumors. Virus-like particles (VLPs), containing tumor antigens, which can immunize against cancers, are cross-presented by dendritic cell (DC) but the mechanism by which this occurs is not fully understood. Here, we used VLPs, derived from rabbit hemorrhagic disease virus (RHDV) with both murine and human DCs, to elucidate these pathways. We have employed inhibitors to demonstrate that these VLPs are taken up by clathrin-dependent macropinocytosis and phagocytosis before being degraded in acidic lysosomal compartments. VLP-derived peptides are loaded onto major histocompatibility complex I that have been recycled from the cell surface. Antigen-coupled VLPs and murine ovalbumin-specific and human melanoma-associated antigen recognized by T cells (MART-1)-specific CD8(+) T cells were used to demonstrate cross-presentation via this alternate, receptor recycling pathway, which operated independently of the proteasome and the transporter-associated with antigen presentation. Finally, we found that cross-presentation of VLPs in vivo was not confined to CD8α(+) DC subsets. These data define the cross-presentation pathway for RHDV VLPs and may lead to improved cancer immunotherapies.
Synchronization properties in multimedia systems (1990)

Multimedia is defined as the integrated generation, representation, processing, storage, and dissemination of independent machine-processable information expressed in multiple time-dependent and time-independent media such as data, graphics, drawings, voice, audio, and video. The characteristics of synchronization mechanisms desirable for central and distributed multimedia systems are addressed. The concept of multimedia objects as components of an object-based model for a multimedia system is introduced. The essential new synchronization requirement is restricted blocking together with synchronization features covering real-time aspects. Existing synchronization mechanisms can be altered or new ones defined to meet these requirements.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Activity of bilobalide, a sesquiterpene from Ginkgo biloba, on Pneumocystis carinii (1993)

The sesquiterpene bilobalide, extracted from Ginkgo biloba leaves, was tested in vitro and in vivo for the ability to inhibit Pneumocystis carinii growth. Bilobalide was inhibitory to trophozoites cultured on human embryonic lung fibroblasts (HEL 299) at approximately the same concentration as trimethoprim plus sulfamethoxazole (lowest effective concentration, 50 micrograms of bilobalide per ml versus 9/45 microgram of trimethoprim-sulfamethoxazole per ml), inducing microscopically detectable morphological changes in the cytoplasm of the parasite. In pharmacologically immunosuppressed Sprague-Dawley rats transtracheally infected with a suspension of about 5 x 10(6) P. carinii trophozoites per ml, the daily intraperitoneal administration of bilobalide (10 mg/kg of body weight for 8 days) lowered the number of organisms by approximately 2 logs (that is, about 99%). There was no apparent toxicity either in uninfected HEL 299 feeder cells or in infected and uninfected animals. These studies suggest that the sesquiterpene bilobalide might be useful for therapy of and prophylaxis against P. carinii infections in humans.
The structure of tight junctions in human thyroid tumors. A systematic freeze-fracture study. (1979)

Tight junctions of normal human epithelium comprise 6--8 intramembranous ridges. Tight junctions of adenomas and one case of follicular carcinoma were morphologically identical with normal controls. Another follicular carcinoma (clinically less malignant than the case mentioned above), however, showed loss of junctional polarity with spreading of ridges on lateral cell membranes. One case of papillary carcinoma (clinically low malignancy) displayed only focal attenuations of the tight junction belt. Another more malignant tumor of the same histologic type showed breaks of ridges and focal interruptions of the tight junction meshwork. Cell membranes of two anaplastic carcinomas of high malignancy were completely devoid of tight junction ridges. We conclude that tight junction alterations are not necessarily implicated in malignant transformation and that they correspond with tumor differentiation rather than directly with a single parameter of thyroid tumor malignancy.
Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma (2012)

Cancer-testis genes are frequently expressed in bladder cancer and especially in tumours of high stage and grade. In addition, the CT gene expression may have both prognostic and predictive value. Development of specific immunotherapy against the CT antigens in bladder cancer may ultimately increase patient survival.
Algorithms for fast vector quantization (2002)

This paper shows that if one is willing to relax the requirement of finding the true nearest neighbor, it is possible to achieve significant improvements in running time and at only a very small loss in the performance of the vector quantizer. The authors present three algorithms for nearest neighbor searching: standard and priority k-d tree search algorithms and a neighborhood graph search algorithm in which a directed graph is constructed for the point set and edges join neighboring points.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>