gebhart/patrepeval huggingface dataset:
Effects of autonomic nerve stimulation, asynchrony, and load on dP/dtmax and on dP/dtmin (1991)

We studied asynchronous depolarization effects on mechanical responses to autonomic nerve stimulation (ANS) in 21 dogs with AV nodal block. In 11 of these dogs, we kept mean aortic pressure (AoP) at 85 mmHg and paced the hearts at 120 beats/min. We paced, in random order, either the LV apex or the RV anterior or lateral wall to increase the asynchrony of ventricular contraction and relaxation. During pacing at each site, we determined maximum rate of LVP rise (dP/dtmax) and fall (dP/dtmin) before and during stimulation of cardiac sympathetic and vagal nerves and during combined nerve stimulation. Changing the pacing site from LV to RV decreased dP/dtmax and also dP/dtmin (P less than 0.001); the decrease was most pronounced when we shifted pacing from LV apex to RV anterior wall. Sympathetic stimulation increased (P less than 0.0001) and vagal stimulation decreased (P less than 0.001) dP/dtmax and dP/dtmin during pacing at each site. Effect of vagal stimulation was substantially enhanced during concomitant sympathetic stimulation. However, the magnitude of changes in dP/dtmax and also in dP/dtmin due to nerve stimulations during pacing at each ventricular site was similar. In 10 additional dog experiments, we also varied AoP from a mean value of 74 to 113 mmHg during pacing at each site. An increase in AoP augmented dP/dtmax by a mean value of 582 mmHg/s and increased dP/dtmin by a mean value of 617 mmHg/s (both P less than 0.0001). Sympathetic stimulation increased, whereas vagal stimulation decreased, dP/dtmax and dP/dtmin at each afterload (P less than 0.001).
Synthesis of a Monocharged Peptide Nucleic Acid (PNA) Analog and Its Recognition as Substrate by DNA Polymerases (1999)

The preparation of a novel phosphoramidite monomer based on thyminyl acetic acid coupled to the secondary nitrogen of 2-(2-amino-ethylamino)ethanol is described. This monomer can be used to attach a deoxynucleotide to the carboxy terminus of a PNA oligomer by solid-phase synthesis. The resulting PNA primer is recognized as a substrate by various DNA polymerases.
Interference systems of controllable mirrors based on vanadium dioxide for the spectral range 06-106 micrometer (1999)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text O. P. Konovalova, A. I. Sidorov, and I. I. Shaganov, "Interference systems of controllable mirrors based on vanadium dioxide for the spectral range 0.6-10.6 micrometer," J. Opt. Technol. 66, 391- (1999) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
The NEF Reaction (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe NEF ReactionWayland E. NolandCite this: Chem. Rev. 1955, 55, 1, 137–155Publication Date (Print):February 1, 1955Publication History Published online1 May 2002Published inissue 1 February 1955https://pubs.acs.org/doi/10.1021/cr50001a003https://doi.org/10.1021/cr50001a003research-articleACS PublicationsRequest reuse permissionsArticle Views2845Altmetric-Citations206LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Selected base sequence outside the target binding site of zinc finger protein Sp1 (2001)

Human transcription factor Sp1 contains three contiguous repeats of the C2H2-type zinc finger motif and binds to the decanucleotide sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box). In order to determine whether the three-zinc finger peptide Sp1(530-623) has selectivity for sequence outside the GC box, we used a selection and amplification of binding experiment. The high affinity sequence generated from this selection is 5'-GGGTGGGCGTGGC-3' (s-GC box), which is flanked by a novel conserved guanine triplet on the 5'-side of the core decanucleotide. Gel mobility shift assays reveal that Sp1(530-623) binds to the s-GC box with 2.3-fold higher affinity than to the wild-type GC box, 5'-GGGGCGGGGC-3' (c-GC box). DNase I and hydroxyl radical footprinting analyses show that the area of the s-GC box protected by binding of Sp1(530-623) is wider by 1 nt than that of the c-GC box. On the other hand, alkylation interference analyses demonstrate that Sp1(530-623) forms only one special base contact at the guanine triplet. With respect to cleavage of the c-GC and s-GC boxes by the 1,10-phenanthroline-copper complex (OP-Cu), binding of Sp1(530-623) has no effect on the cleavage pattern of the s-GC box, whereas OP-Cu actually enhances cleavage of the c-GC box. Additionally, the extent of cleavage of the s-GC box by DNase I and OP-Cu is clearly different from that of the c-GC box under peptide-free conditions. The results strongly indicate that: (i) the conformation of the s-GC box is evidently distinct from that of the c-GC box; (ii) Sp1(530-623) binds to the s-GC box without induction of a conformational change in DNA detectable by cleavage with OP-Cu. The present study provides useful information for the design of multi-zinc finger proteins with various sequence specificities.
Spatio‐temporal prediction of snow cover in the Black Forest mountain range using remote sensing and a recurrent neural network (2009)

Abstract Winter tourism is the main economic factor for many different regions in the German Mountain Range. Owing to warming trends experienced in the past and predicted for the future, precise knowledge about the development of snow cover and snow duration in the future is becoming more and more important. On the basis of the International Panel on Climate Change (IPCC) A1B scenario, this paper investigates the possible regional development of snow cover and snow duration in the Black Forest in southwest Germany until 2050. For this purpose, we developed a new method that combines Non‐linear AutoRegressive networks with eXogenous inputs (NARX) with Remote Sensing and Geographic Information System (GIS). With this non‐parametric approach, we try to define with preferably high accuracy a simple transferable model. Besides the general problem of developing a robust statistical model, our main focus is on the enhancement of the spatial resolution of snow patterns by incorporating complex structures of the underlying terrain using Moderate Resolution Imaging Spectroradiometer (MODIS) satellite data. The results suggest a possible decrease in the number of snow days (snow cover ≥ 10 cm) in the decade 2041–2050 by 10 to 44% at altitudes higher than 1200 m, by 17 to 57% at 1000–1200 m and 25 to 66% at 500–1000 m. This results in a dramatic shortening of the snow season mainly caused by earlier snow melt initiation rather than by later first snow precipitation in autumn. These considerable changes in the snow season would cause enormous losses in the skiing and tourism industries. In this context, the obtained high‐resolution snow data and maps can provide a useful tool and decision‐making aid for the economy and politics. Copyright © 2009 Royal Meteorological Society
A new conjugated linoleic acid isomer, 7 trans, 9 cis‐octadecadienoic acid, in cow milk, cheese, beef and human milk and adipose tissue (1998)

The identity of a previously unrecognized conjugated linoleic acid (CLA) isomer, 7 trans, 9 cis-octadecadienoic acid (18:2) was confirmed in milk, cheese, beef, human milk, and human adipose tissue. The 7 trans, 9 cis-18:2 isomer was resolved chromatographically as the methyl ester by silver ion-high-performance liquid chromatography (Ag+-HPLC); it eluted after the major 9 cis, 11 trans-18:2 isomer (rumenic acid) in the natural products analyzed. In the biological matrices investigated by Ag+-HPLC, the 7 trans, 9 cis-18:2 peak was generally due to the most abundant minor CLA isomer, ranging in concentration from 3 to 16% of total CLA. By gas chromatography (GC) with long polar capillary columns, the methyl ester of 7 trans, 9 cis-18:2 was shown to elute near the leading edge of the major 9 cis, 11 trans-18:2 peak, while the 4,4-dimethyloxazoline (DMOX) derivative permitted partial resolution of these two CLA isomers. The DMOX derivative of this new CLA isomer was analyzed by gas chromatography-electron ionization mass spectrometry (GC-EIMS). The double bond positions were at delta7 and delta9 as indicated by the characteristic mass spectral fragment ions at m/z 168, 180, 194, and 206, and their allylic cleavages at m/z 154 and 234. The cis/trans double-bond configuration was established by GC-direct deposition-Fourier transform infrared as evidenced from the doublet at 988 and 949 cm(-1) and absorptions at 3020 and 3002 cm(-1). The 7 trans, 9 cis-18:2 configuration was established by GC-EIMS for the DMOX derivative of the natural products examined, and by comparison to a similar product obtained from treatment of a mixture of methyl 8-hydroxy- and 11-hydroxyoctadec-9 cis enoates with BF3 in methanol.
Absorbable ligating clips. (1982)

Surgical clips have gained wide spread acceptance because of speed and utility in operative fields with difficult exposure. Certain disadvantages make metallic clips less than optimal for many operative situations. Such problems include difficulty in loading metallic clips into the applier, clips falling from the applier in the transfer from nurse to surgeon or while the surgeon is positioning the clip for application and clips coming off a vessel to which it was applied. The interference which metallic clips produce in computerized tomography and their permanency must be added as factors to be evaluated in comparison with absorbable clips.
Surgical treatment of intracranial aneurysms: six-year experience in Belo Horizonte, MG, Brazil (2004)

Spontaneous subarachnoid hemorrhage accounts for 5 to 10 % of all strokes, with a worldwide incidence of 10.5 / 100000 person/year, varying in individual reports from 1.1 to 96 /100000 person/year. Angiographic and autopsy studies suggest that between 0.5% and 5% of the population have intracranial aneurysms. Approximately 30000 people suffer aneurysmal subarachnoid hemorrhage in the United States each year, and 60% die or are left permanently disabled. We report our experience in the surgical treatment of intracranial aneurysms in a six year period, in Belo Horizonte, Minas Gerais, Brazil. We reviewed the hospital files, surgical and out-patient notes of all patients operated on for the treatment of intracranial aneurysms from January 1997 to January 2003. Four hundred and seventy-seven patients were submitted to 525 craniotomies for treatment of 630 intracranial aneurysms. The majority of patients were female (72.1%) in the fourth or fifth decade of life. Anterior circulation aneurysms were more common (94.4%). The most common location for the aneurysm was the middle cerebral artery bifurcation. The patients were followed by a period from 1 month to 5 years. The outcome was measured by the Glasgow Outcome Scale (GOS). At discharge, 62.1% of the patients were classified as GOS 5, 13.9% as GOS 4, 8.7% as GOS 3, 1.7% as GOS 2 and 14.8% as GOS 1.
An empirical study of secure MPEG video transmissions (2002)

MPEG (Moving Pictures Expert Group) is an industrial standard for video processing and is widely used in multimedia applications in the Internet. However, no security provision is specified in the standard. We conducted an experimental study of previously proposed selective encryption schemes for MPEG video security. This study showed that these methods are inadequate for sensitive applications. We discuss the tradeoffs between levels of security and computational and compression efficiency.
Thetransformergene inCeratitis capitataprovides a genetic basis for selecting and remembering the sexual fate (2002)

The medfly Ceratitis capitata contains a gene (Cctra) with structural and functional homology to the Drosophila melanogaster sex-determining gene transformer (tra). Similar to tra in Drosophila, Cctra is regulated by alternative splicing such that only females can encode a full-length protein. In contrast to Drosophila, however, where tra is a subordinate target of Sex-lethal (Sxl), Cctra seems to initiate an autoregulatory mechanism in XX embryos that provides continuous tra female-specific function and act as a cellular memory maintaining the female pathway. Indeed, a transient interference with Cctra expression in XX embryos by RNAi treatment can cause complete sexual transformation of both germline and soma in adult flies, resulting in a fertile male XX phenotype. The male pathway seems to result when Cctra autoregulation is prevented and instead splice variants with truncated open reading frames are produced. We propose that this repression is achieved by the Y-linked male-determining factor (M).
Recombinant Capsular Antigen (Fraction 1) from Yersinia Pestis Induces a Protective Antibody Response in BALB/c Mice (1990)

Yersinia pestis produces a glycoprotein capsule, the biosynthesis of which appears to be temperature dependent. The fraction I (F1) component of this capsule is specific to Y. pestis and the detection of F1 antibodies is the basis for several serological tests. We report the cloning of the F1 gene and its expression in Escherichia coli using the phagemid vector lambda ZAPII and a F1-specific monoclonal antibody. The recombinant F1 antigen had a molecular weight of 17 kDa, which proved to be identical to that of the F1 antigen produced by Y. pestis. The recombinant cells produced F1 antigen at 37 degrees C but only minimal amounts at 27 degrees C, suggesting that the genetic features affected by temperature in Y. pestis may be operating in the E. coli clone. It is not known if their similar molecular weights reflect the glycosylated nature of both proteins. F1 antigen purified from the E. coli recombinant induced a protective immune response in BALB/c mice challenged with up to 10(5) virulent Y. pestis. The resistance of immunized mice to plague infection correlated with high titers of F1 antibody. The cloned gene expresses an immunogenically competent F1 antigen suitable for use in plague serodiagnostics and vaccine development.
Rapid HPLC assay for the .beta.-exotoxin of Bacillus thuringiensis (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRapid HPLC assay for the .beta.-exotoxin of Bacillus thuringiensisDavid P. Campbell, Donald E. Dieball, and John M. BrackettCite this: J. Agric. Food Chem. 1987, 35, 1, 156–158Publication Date (Print):January 1, 1987Publication History Published online1 May 2002Published inissue 1 January 1987https://pubs.acs.org/doi/10.1021/jf00073a033https://doi.org/10.1021/jf00073a033research-articleACS PublicationsRequest reuse permissionsArticle Views163Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Progressive horizon planning-planning exploratory-corrective behavior (1993)

TraumAID is a consultation system for the diagnosis and treatment of multiple trauma. It integrates diagnostic reasoning, planning, and action. Its reasoner identifies diagnostic and therapeutic goals appropriate to the physician's knowledge of the patient's state, while its planner advises on beneficial actions to next perform. The physician's lack of complete knowledge of the situation and the time limitations of emergency medicine constrain the ability of any planner to identify what would be the best thing to do. TraumAID's Progressive Horizon Planner has been designed to create a plan for patient care that is in keeping with the standards of managing trauma.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Exploring Strategies for the Design of Artificial Transcription Factors (2004)

Artificial transcription factors can be engineered to interact with specific DNA sequences to modulate endogenous gene expression within cells. A significant hurdle to implementation of this approach is the selection of the appropriate DNA sequence for targeting. We reasoned that a good target site should be located in chromatin, where it is accessible to DNA-binding proteins, and it should be in the close vicinity of known transcriptional regulators of the gene. Here we have explored the efficacy of these criteria to guide our selection of potential regulators of gamma-globin expression. Several zinc finger-based transcriptional activators were designed to target the sites proximal to the -117-position of the gamma-globin promoter. This region is proximal to the binding sites of known and potential natural transcription factors. Design and study of three transcription factors identified the potent transcriptional activator, gg1-VP64-HA. This transcription factor was able to interact directly with the gamma-globin promoter and up-regulate expression of reporter gene constructs as well as the endogenous gene in a selective manner. Transfection of a gg1-VP64-HA expression vector or retroviral delivery of this transcription factor into the erythroleukemia cell line K562 resulted in an increase of fetal hemoglobin. The gamma-globin content of cells expressing gg1-vp64-HA showed up to 16-fold higher levels of fetal hemoglobin than the native K562 cell line. These transcriptional activators constitute a novel class of regulators of the globin locus that may be suitable for treatment of diseases arising from mutations in this locus such as sickle cell disease and thalassemic diseases.
In vivo inhibition of the pyrimidine de novo enzyme dihydroorotic acid dehydrogenase by brequinar sodium (DUP-785; NSC 368390) in mice and patients. (1990)

Little is known about the in vivo effects of inhibition of the mitochondrial pyrimidine de novo synthesis enzyme dihydroorotic acid dehydrogenase (DHO-DH). In mice a new inhibitor of DHO-DH, Brequinar sodium (DUP-785, NSC 368390) depleted the plasma uridine concentration to 40% within 2 h, followed by a small rebound after 7-9 days. The drug was subsequently evaluated in a Phase I clinical trial, during which it was possible to follow its biochemical effects in 24 patients (27 courses). In addition to the measurement of plasma uridine concentrations, we also measured in lymphocytes of 9 patients (10 courses) the duration of DHO-DH inhibition. Brequinar sodium was administered every 3 weeks as an i.v. infusion at dose levels of 15-2250 mg/m2. The biochemical effects were studied following the first administration of the drug. In sonicated extracts of lymphocytes from 7 healthy volunteers the activity of DHO-DH varied from 2.0 to 3.9 nmol/h per 10(6) cells, while in the lymphocytes of 9 patients obtained immediately before treatment this value was between 0.5 and 4.8 nmol/h per 10(6) cells. Within 15 min of drug administration DHO-DH activity was not detectable and was still low up to 1 week later. Duration of the inhibition appeared to be related to the extent of clinical toxicity, e.g., myelosuppression, nausea, vomiting, diarrhea, and mucositis. Severe lymphopenia was observed in patients receiving Brequinar sodium at the maximum tolerated dose. At dose levels of greater than or equal to 600 mg/m2, uridine depletion (40-85%) was observed between 6 h and 4 days, followed by a rebound of 160-350% after 4-7 days. The extent of the depletion and of the accompanying rebound of uridine levels and the extent and duration of DHO-DH inhibition in the individual patients could be partially associated with drug toxicity in these patients. This is the first report describing biological effects of DHO-DH inhibition in humans in relation to the degree and duration of inhibition of this enzyme.
Second genital primary squamous neoplasms in vulvar carcinoma: viral and histopathologic correlates. (1993)

To determine whether vulvar squamous cell carcinomas associated with certain morphologic features and/or human papillomavirus (HPV) nucleic acids were more likely to be associated with other genital primary squamous neoplasms.We surveyed 169 invasive squamous cell carcinomas of the vulva and correlated associated vulvar intraepithelial neoplasia (VIN), invasive growth patterns resembling VIN (intraepithelial-like or basaloid), and the presence of HPV nucleic acids by in situ hybridization with a history of a second primary squamous neoplasm of the genital tract.Twenty-two patients (13%) had a history of a second primary. An intraepithelial growth pattern or an associated VIN correlated significantly with HPV, at P = .0005 and P = .007, respectively, and with a second primary, at P = .077 and P = .009, respectively. When HPV-positive, the same histologic variables correlated with a second primary at P = .099 and P = .25, respectively. Compared with cases lacking both these histologic features and HPV, they correlated with multifocal disease at P = .01 and P = .003.The findings of HPV nucleic acids, tumor growth patterns, and associated VIN are interrelated and confer risk of other genital primary neoplasms in women with vulvar carcinoma. This supports the concept that subsets of vulvar carcinoma may be distinguished not only by morphology and HPV DNA, but also by a distinctly different risk of a second genital primary neoplasm.
Performance Enhancement of Whistle Sound Source Tracking Algorithm using Time-Scale Filter Based on Wavelet Transform (2004)

A purpose of developing a sound source tracking system in this paper is to reduce the noise efficiently from the received signal by microphone array and measure the signal's time delay between the microphones. I have applied the wavelet analysis algorithm to the system and calculated the sound source's relative position For the performance evaluation, I have compared with the results of utilizing the digital filtering methods based on the FIR LPF using Kaiser window function and the inverse Chebyshev IIR LPF. As a result, I have confirmed the fact that 'time-scale' filter using inverse discrete wavelet transform was suitable for this system.