gebhart/patrepeval huggingface dataset:
In vitro Hepatic Differentiation of Human Mesenchymal Stem Cells (2004)

This study examined whether mesenchymal stem cells (MSCs), which are stem cells originated from embryonic mesoderm, are able to differentiate into functional hepatocyte-like cells in vitro. MSCs were isolated from human bone marrow and umbilical cord blood, and the surface phenotype and the mesodermal multilineage differentiation potentials of these cells were characterized and tested. To effectively induce hepatic differentiation, we designed a novel 2-step protocol with the use of hepatocyte growth factor and oncostatin M. After 4 weeks of induction, cuboidal morphology, which is characteristic of hepatocytes, was observed, and cells also expressed marker genes specific of liver cells in a time-dependent manner. Differentiated cells further demonstrated in vitro functions characteristic of liver cells, including albumin production, glycogen storage, urea secretion, uptake of low-density lipoprotein, and phenobarbital-inducible cytochrome P450 activity. In conclusion, human MSCs from different sources are able to differentiate into functional hepatocyte-like cells and, hence, may serve as a cell source for tissue engineering and cell therapy of hepatic tissues. Furthermore, the broad differentiation potential of MSCs indicates that a revision of the definition may be required.
Augmenting a microprocessor with reconfigurable hardware (2000)

As VLSI technology continues to improve, configurable hardware devices such as PLDs are progressively replacing many specialized digital integrated circuits. Field-programmable gate arrays (FPGAs) are one class of such devices, characterized by their ability to be reconfigured as often as desired. Lately, FPGAs have advanced to the stage where they can host large computational circuits, giving rise to the study of reconfigurable computing as a potential alternative to traditional microprocessors. Most previous reconfigurable computers, however, have been ad hoc designs that are not fully compatible with existing general-purpose computing paradigms. This thesis
The non-pharmacological treatment of osteoarthritis (1995)

The term osteoarthritis embraces a wide spectrum of clinical conditions with the common feature of
DNA Repair and Cell Cycle Control Genes and the Risk of Young-Onset Lung Cancer (2006)

Exposure to tobacco smoke and to mutagenic xenobiotics can cause various types of DNA damage in lung cells, which, if not corrected by DNA repair systems, may lead to deregulation of the cell cycle and, ultimately, to cancer. Genetic variation could thus be an important factor in determining susceptibility to tobacco-induced lung cancer with genetic susceptibility playing a larger role in young-onset cases compared with that in the general population. We have therefore studied 102 single-nucleotide polymorphisms (SNP) in 34 key DNA repair and cell cycle control genes in 299 lung cancer cases diagnosed before the age of 50 years and 317 controls from six countries of Central and Eastern Europe. We have found no association of lung cancer risk with polymorphisms in genes related to cell cycle control, single-strand/double-strand break repair, or base excision repair. Significant associations (P < 0.05) were found with polymorphisms in genes involved in DNA damage sensing (ATM) and, interestingly, in four genes encoding proteins involved in mismatch repair (LIG1, LIG3, MLH1, and MSH6). The strongest associations were observed with heterozygote carriers of LIG1 -7C>T [odds ratio (OR), 1.73; 95% confidence interval (95% CI), 1.13-2.64] and homozygote carriers of LIG3 rs1052536 (OR, 2.05; 95% CI, 1.25-3.38). Consideration of the relatively large number of markers assessed diminishes the significance of these findings; thus, these SNPs should be considered promising candidates for further investigation in other independent populations.
FIELD STUDY OF A PEDESTRIAN BRIDGE OF REINFORCED PLASTIC (1987)

A discussion of the behavior of the superstructure of a pedestrian bridge fabricated with glass-reinforced plastic under a field load test is presented. Experimental measurements of elastic vertical deflections were 1.8 times greater than those predicted by means of a finite element solution. A live load of 3.5 times the dead load of the superstructure and polymer concrete deck was used for the elastic load test. Elastic strains were uniform among the different elements of the superstructure and computed stresses did not exceed 10,000 lbf/sq in. at full live load. A residual deflection in the superstructure of 0.10 in. on removal of the live load was concentrated in the supports. Creep deflection and strain measurements recorded over 61 days indicated that negligible creep occurred under a load of 2.6 times the dead load. Air temperature variations produced pronounced changes in deflection and strain readings, but were reversible. The overall structural behavior of the bridge and resistance to handling abuse exceeded expectations.
Targeting epidermal growth factor receptor variant III: a novel strategy for the therapy of malignant glioma (2007)

Immunotherapy represents an exciting approach for the treatment of glioblastoma multiforme. The success of this approach depends on the identification of tumor-specific antigens that can then be exploited to target the tumor. One such antigen, the epidermal growth factor receptor variant III (EGFRvIII) is found in up to 50% of malignant gliomas. This molecule is an interesting vaccine candidate since its expression is tumor specific. Recent preclinical, as well as clinical, studies of EGFRvIII-based vaccines suggest a promising role for immunotherapy of malignant gliomas. This review focuses on vaccines that target EGFRvIII. We review preclinical and clinical data and emphasize the immunological mechanisms involved in its therapeutic effect.
Communicating with Calcium (1999)

### Calcium as a Ubiquitous Signal in Plants

All living cells use a network of signal transduction pathways to conduct developmental programs, obtain nutrients, control their metabolism, and cope with their environment. A major challenge for cell biologists is to understand the “language” of
Contrast enhancement methods for optical coherence tomography (2004)

Contrast in optical coherence tomography (OCT) images is often limited, particularly when pathological tissue is morphologically or optically similar to normal tissue. We present novel contrast enhancing methods designed to selectively identify tissues of interest.
Control of Self-Assembly of DNA Tubules Through Integration of Gold Nanoparticles (2009)

The assembly of nanoparticles into three-dimensional (3D) architectures could allow for greater control of the interactions between these particles or with molecules. DNA tubes are known to form through either self-association of multi-helix DNA bundle structures or closing up of 2D DNA tile lattices. By the attachment of single-stranded DNA to gold nanoparticles, nanotubes of various 3D architectures can form, ranging in shape from stacked rings to single spirals, double spirals, and nested spirals. The nanoparticles are active elements that control the preference for specific tube conformations through size-dependent steric repulsion effects. For example, we can control the tube assembly to favor stacked-ring structures using 10-nanometer gold nanoparticles. Electron tomography revealed a left-handed chirality in the spiral tubes, double-wall tube features, and conformational transitions between tubes.
In-fibre Bragg grating sensors (1997)

In-fibre Bragg grating (FBG) sensors are one of the most exciting developments in the field of optical fibre sensors in recent years. Compared with conventional fibre-optic sensors, FBG sensors have a number of distinguishing advantages. Significant progress has been made in applications to strain and temperature measurements. FBG sensors prove to be one of the most promising candidates for fibre-optic smart structures. This article presents a comprehensive and systematic overview of FBG sensor technology regarding many aspects including sensing principles, properties, fabrication, interrogation and multiplexing of FBG sensors. It is anticipated that FBG sensor systems will be commercialized and widely applied in practice in the near future due to the maturity of economical production of FBGs and the availability of cost effective interrogation and multiplexing techniques.
IL-12 activates IFN-gamma production through the preferential activation of CD30+ T cells. (1995)

CD30 is normally expressed by a subset (15 to 20%) of CD45RO+ T cells after activation by a variety of T cell stimuli and defines the principal IFN-gamma-producing T cells subset. Inasmuch as the production of IFN-gamma is regulated by IL-2 and IL-12, we have examined the effects of these cytokines on the proliferation, induction, and function of CD30+ and CD30- T cell subsets. Upon isolation, CD30+CD25+ T cells exhibit high levels of baseline proliferation compared with CD30-CD25+ T cells. Neutralizing Abs specific for IL-2, IL-4, IL-6, or IL-12 had no effect on basal levels of proliferation in CD30+CD25+ T cells, whereas anti-IL-2 inhibited the basal proliferative activity of CD30-CD25+ T cells. The addition of exogenous rIL-12 to purified CD30+CD25+ and CD30-CD25+ T cells subsets induced significantly higher maximal levels of proliferation in the CD30+ subset, whereas rIL-2 supported comparable levels of maximal proliferation in each subset. The addition of rIL-2 to anti-CD3-activated PBMC increased the relative numbers of CD30+ T cells by expansion of CD30+ T cells, as opposed to recruitment of CD30+ T cells from the CD30- population. Furthermore, the development of the CD30+ T cell subset was inhibited by either anti-IL-2, anti-IL-12, or rIL-10. Inhibition by anti-IL-2 and rIL-10 was overcome by the addition of rIL-12, but not IL-2, to the cultures. Finally, rIL-12 increased IFN-gamma production by CD30+ T cells, yet had little effect on IFN-gamma production by CD30- T cells. Thus, CD30+ T cells are preferentially regulated by IL-12, and the effects of IL-12 on T cell IFN-gamma production are mediated largely through its effects on the CD30+ subset.
Elevated Function of Blood Clotting Factor VIIa Mutants That Have Enhanced Affinity for Membranes (2001)

Blood clotting factor VIIa is involved in the first step of the blood coagulation cascade, as a membrane-associated enzyme in complex with tissue factor (TF). Factor VIIa is also an important therapeutic agent for hemophilia where its function may include TF-independent as well as TF-dependent mechanisms. This study compared the activity of wild type factor VIIa (WT-VIIa) with that of a mutant with elevated affinity for membrane (P10Q/Q32E, QE-VIIa). Phospholipid and cell-based assays showed the mutant to have up to 40-fold higher function than WT-VIIa in both TF-dependent and TF-independent reactions. Tissue factor-dependent reactions displayed the maximum enhancement when binding had reached equilibrium in competition with another TF-binding protein. In liposome-based assays, the association rate of WT-VIIa with TF occurred at a physical maximum and could not be improved by site-directed mutagenesis. A practical consequence was identical function of WT-VIIa and QE-VIIa in assays that depended entirely on assembly kinetics. Thus, factor VIIa mutants provided unique reagents for probing the mechanism of factor VIIa action. They may also offer superior agents for therapy.
Database (1983)

This paper proposes a procedure for determining whether a database management system should be used for the implementation of some particular computer application. The procedure calculates the present value of the expected costs for development and maintenance of the system for each of its implementation methods. The procedure is most applicable to data processing shops that use database management systems but that also acknowledge the pertinence of standard file access methods to some applications. The paper compares this procedure to other procedures for making such a determination.
The Evolution of a Capacity to Build Supra-Cellular Ropes Enabled Filamentous Cyanobacteria to Colonize Highly Erodible Substrates (2009)

Rope building is a supracellular morphological adaptation in filamentous cyanobacteria that allows them to colonize physically unstable sedimentary environments, and to act as successful pioneers in the biostabilization process.
Neurophysiological Aspects of Penile Erection: the Role of the Sympathetic Nervous System (1989)

Recent studies have revealed that the initiation, maintenance and rigidity of penile erection are under parasympathetic and somatotopic control. The mechanism of penile detumescence is still under debate. Single and combined cavernous and hypogastric nerve stimulation was used to study the influence of the sympathetic nervous system on penile erection and to identify the mechanism of penile detumescence. Penile arterial flow and intracorporeal pressure recordings during various stimulation patterns indicated that erection induced by stimulation of the cavernous nerve could be blocked by excitation of the upper hypogastric nerves. Venous outflow studies demonstrated that the inhibitory effect of the sympathetic nervous system was due to active vaso- and corporeal smooth muscle constriction. It was concluded that subsidence and suppression of erection is a sympathetic function. Previous studies have shown that the neurophysiology of erection can be explained by 3 major inhibitory (sympathetic). Our findings are of clinical importance, since a better appreciation of the basic erectile mechanism is essential for successful management of erectile dysfunction.
Evaluation of the fibroblast growth factor system as a potential target for therapy in human prostate cancer (2005)

Overexpression of fibroblast growth factors (FGFs) has been implicated in prostate carcinogenesis. FGFs function via their high-affinity interactions with receptor tyrosine kinases, FGFR1-4. Expression of FGFR1 and FGFR2 in prostate cancer (CaP) was not found to be associated with clinical parameters. In this report, we further investigated for abnormal FGFR expression in prostate cancer and explore their significance as a potential target for therapy. The expression levels of FGFR3 and FGFR4 in CaP were examined and corroborated to clinical parameters. FGFR3 immunoreactivity in benign prostatic hyperplasia (BPH) and CaP (n=26 and 57, respectively) had similar intensity and pattern. Overall, FGFR4 expression was significantly upregulated in CaP when compared to BPH. A significant positive correlation between FGFR4 expression and Gleason score was noted: Gleason score 7-10 tumours compared to BPH (P<0.0001, Fisher's exact test), Gleason score 4-6 tumours compared to BPH (P<0.0004), and Gleason 7-10 compared to Gleason 4-6 tumours (P<0.005). FGFR4 overexpression was associated with an unfavourable outcome with decreased disease-specific survival (P<0.04, log rank test). FGF-induced signalling is targeted using soluble FGF receptor (sFGFR), potent inhibitor of FGFR function. We have previously shown that sFGFR expression via a replication-deficient adenoviral vector (AdlllcRl) suppresses in vitro FGF-induced signalling and function in human CaP DU145 cells. We tested the significance of inhibiting FGF function along with conventional therapeutic modalities in CaP, and confirmed synergistic effects on in vitro cell growth (proliferation and colony formation) by combining sFGFR expression and treatment with either Paclitaxel (Taxol) or gamma-irradiation. In summary, our data support the model of FGF system as valid target for therapy in CaP.
Modification of blood flow in the HSN tumour and normal tissues of the rat by the endothelin ETB receptor agonist, IRL 1620 (1999)

Activation of endothelin receptors on the vasculature can produce a variety of responses from potent vasoconstriction to mild vasodilation, depending on the receptor complement within the tissue. To elucidate the potential role of endothelin analogues as tumour blood flow modifiers, we have evaluated the effect of the ETB receptor agonist, IRL 1620 ([Suc-(Glu9, Ala11,15)-ET-1(8-21)]) in CBH/CBi rats bearing an HSN fibrosarcoma. Tissue blood flow and vascular resistance were determined, 20 min following administration of IRL 1620 (bolus intravenous), using the uptake of radiolabelled iodoantipyrine (125I-IAP). Blood flow was unchanged in most tissues. However, at doses ≥1.0 nmol kg−1 IRL 1620, blood flow in the brain and heart was increased, whereas in the small intestine it was reduced. Blood flow in the skeletal muscle was reduced at 1.0 nmol kg−1 only. Tumour blood flow was significantly reduced at 3.0 and 5.0 nmol kg−1. Vascular resistance was unchanged in most tissues although it was increased in the skeletal muscle at 1.0 nmol kg−1, in the kidney at 1.0 and 3.0 nmol kg−1 and in the brain and heart, it was reduced at 5.0 nmol kg−1 IRL 1620. Vascular resistance was significantly increased in the tumour and the small intestine at doses ≥1 nmol kg−1 IRL 1620. Pretreatment of rats with BQ-788, an ETB receptor antagonist, selectively attenuated the tumour vascular response to 3 nmol kg−1 IRL 1620 with no changes observed in the normal tissue responses. Our results demonstrate that the HSN tumour vasculature is selectively responsive to IRL 1620 at doses >1 nmol kg−1 compared with the majority of normal tissues with the exception of the small intestine, and that only the tumour response is highly sensitive to BQ-788 antagonism, under the experimental dosing regime investigated. These differences may be exploitable for therapeutic benefit. Int. J. Cancer 80:295–302, 1999. © 1999 Wiley-Liss, Inc.
Norepinephrine-evoked pain in fibromyalgia. A randomized pilot study ISCRTN70707830 (2002)

Fibromyalgia patients have norepinephrine-evoked pain. This finding supports the hypothesis that fibromyalgia may be a sympathetically maintained pain syndrome.
Application of Multiplicative Regularization to the Finite-Element Contrast Source Inversion Method (2011)

Multiplicative regularization is applied to the finite-element contrast source inversion (FEM-CSI) algorithm recently developed for microwave tomography. It is described for the two-dimensional (2D) transverse-magnetic (TM) case and tested by inverting experimental data where the fields can be approximated as TM. The unknown contrast, which is to be reconstructed, is represented using nodal variables and first-order basis functions on triangular elements; the same first-order basis functions used in the FEM solution of the accompanying field problem. This approach is different from other MR-CSI implementations where the contrast variables are located on a uniform grid of rectangular cells and represented using pulse basis functions. The linear basis function representation of the contrast makes it difficult to apply the weighted <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$L_{2}$</tex></formula> -norm total variation multiplicative regularization which requires that gradient and divergence operators be applied to the predicted contrast at each iteration of the inversion algorithm; the use of finite-difference operators for this purpose becomes unwieldy. Thus, a new technique is introduced to perform these operators on the triangular mesh.
Effect of isoflayones genistein and daidzein in the inhibition of lung metastasis in mice induced by B16F‐10 melanoma cells (1998)

Two dietary soybean isoflavones, genistein and daidzein, were studied for the inhibition of lung metastasis induced by B16F-10 melanoma cells in C57BL/6 mice. The isoflavone genistein at 200 mumol/kg body wt significantly inhibited (53.6%, p < 0.001) lung tumor nodule formation. The lung collagen hydroxyproline content and the serum sialic acid level, a marker of metastasis, were also significantly lower in these animals than in untreated tumor-bearing animals. Genistein treatment also increased the life span of the tumor-bearing animals (47.7%, p < 0.001). The isoflavone daidzein had no significant effect on the reduction of lung metastasis induced by B16F-10 melanoma cells.
Performance trends for analog to digital converters (1999)

Analog-to-digital converters are key components of signal processing systems, and may even dictate system architectures due to their limitations on sampling rate and resolution. The state of the art for ADCs, including both experimental converters and commercially available parts, is reviewed. The data imply that the performance measure, P=2/sup SNRbits//spl middot/f/sub samp/, uncertainty in the sampling process (aperture jitter) over a very wide range of sampling rates. For ADCs operating at multi-GSPS rates, the speed of the device technology is also a limiting factor (due to comparator ambiguity). Technological progress as measured by P is discussed.
O Glycosylation of an Sp1-Derived Peptide Blocks Known Sp1 Protein Interactions (1997)

The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins is dynamic and abundant in the nucleus and cytosol. Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not been determined. In this paper we describe the use of the previously characterized glutamine-rich transactivation domain of Sp1 (B-c) as a model to investigate the role of O-GlcNAc in Sp1's transcriptionally relevant protein-to-protein interactions with the TATA-binding-protein-associated factor (TAF110) and holo-Sp1. When the model Sp1 peptide was overexpressed in primate cells, this 97-amino-acid domain of Sp1 was found to contain a dominant O-GlcNAc residue at high stoichiometry, which allowed the mapping and mutagenesis of this glycosylation site. In vitro interaction studies between this segment of Sp1 and Drosophila TAF110 or holo-Sp1 indicate that the O-GlcNAc modification functions to inhibit the largely hydrophobic interactions between these proteins. In HeLa cells, the mutation at the mapped glycosylation site was permissive for transcriptional activation. We propose the hypothesis that the removal of O-GlcNAc from an interaction domain can be a signal for protein association. O-GlcNAc may thereby prevent untimely and ectopic interactions.