gebhart/patrepeval huggingface dataset:
Modulation by prednisolone of calcium handling in skeletal muscle cells (1995)

1. Increased calcium (Ca2+) influx has been incriminated as a potential pathological mechanism in the chronic skeletal muscle degeneration exhibited by Duchenne muscular dystrophy (DMD) patients. We have studied the influence of the glucocorticoid alpha-methylprednisolone (PDN), the only drug known to have a beneficial effect on the degenerative course of DMD, on Ca2+ handling in the C2 skeletal muscle cell line. 2. PDN, when added 3 days (when myoblasts start to fuse into myotubes) after cell seeding, led to a 2 to 4 fold decrease in cellular Ca2+ uptake. This decrease was independent of the extracellular Ca2+ concentration applied to cells. The effect took at least 24 h in order to become established (PDN of 10(-5) M) and took longer for lower PDN concentrations (EC50 of ca. 10(-6) M at day 5, 10(-6.5) M at day 7 and 10(-7.5) M at day 9 in culture). 3. Cellular calcium accumulation was also decreased in PDN-treated myotubes exposed to 45Ca(2+)-containing medium for 1 to 6 days. 4. No effect of PDN was seen on 45Ca2+ efflux; a decrease in the amount of 45Ca2+ released was observed due to the reduction of cellular 45Ca2+ loading. 5. PDN treatment led to an approximately 2 fold decrease in basal cytosolic Ca2+ concentration. 6. Three antioxidant drugs (lazaroids), previously shown to enhance in vitro skeletal muscle cell differentiation to the same extent as PDN, induced a similar decrease in Ca2+ influx. 7. Our results suggest that long-term incubation of C2 cells with PDN leads to a decrease of the size of the cellular Ca2+ pools and to reduced resting cytosolic Ca2+ levels. Part of the beneficial effect of PDN in DMD patients could be attributed to a reduction of Ca2+ influx and of the size of Ca2+ pools in dystrophic muscle fibres.
An Immunoradiometric Assay for Factor III (Tissue Thromboplastin) (1978)

A solid-phase immunoradiometric assay for tissue thromboplastin (factor III) has been established based on its displacing effect on the binding of 125I-labelled factor III-antibodies to polyvinyl tubes coated with the purified protein component of factor III (apoprotein III). By this method circulating tissue thromboplastin can be detected in experimental animals receiving infusions of crude or purified tissue thromboplastin and in patients undergoing major orthopaedic surgery.
Preclinical Safety Evaluation of AAV2-sFLT01— A Gene Therapy for Age-related Macular Degeneration (2010)

AAV2-sFLT01 is a vector that expresses a modified soluble Flt1 receptor designed to neutralize the proangiogenic activities of vascular endothelial growth factor (VEGF) for treatment of age-related macular degeneration (AMD) via an intravitreal injection. Owing to minimal data available for the intravitreal route of administration for adeno-associated virus (AAV), we initiated a 12-month safety study of AAV2-sFLT01 administered intravitreally at doses of 2.4 × 10(9) vector genomes (vg) and 2.4 × 10(10) vg to cynomolgus monkeys. Expression of sFlt01 protein peaked at ~1-month postadministration and remained relatively constant for the remainder of the study. Electroretinograms, fluorescein angiograms, and tonometry were assessed every 3 months, with no test article-related findings observed in any group. Indirect ophthalmoscopy and slit lamp exams performed monthly revealed a mild to moderate but self-resolving vitreal inflammation in the high-dose group only, which follow-up studies suggest was directed against the AAV2 capsid. Histological evaluation revealed no structural changes in any part of the eye and occasional inflammatory cells in the trabecular meshwork, vitreous and retina in the high-dose group. Biodistribution analysis in rats and monkeys found only trace amounts of vector outside the injected eye. In summary, these studies found AAV2-sFLT01 to be well-tolerated, localized, and capable of long-term expression.
Campiello— new user interface approaches for community networks (1999)

Existing community networks have a broad agenda of using technology to foster social objectives of community cohesion, enhanced education and strong democracy. They are intended to advance social goals such as building community awareness, encouraging involvement in local decision making, or developing economic opportunities in disadvantaged communities [Schuler94].Given these objectives, the availability and modality of access can be considered a major issue, because only a broad participation to the community network activities can sustain their growth and wealth. However the experience so far demonstrate that the common user base is mainly composed by computer literate, accessing the network because the already have a PC at home or at the workplace. In fact from the technology point of view community networks are based on large bulletin boards and the main user interface is usually PC-based (proprietary application), a Web browser.In this paper we briefly present some ideas from the EU funded project Campiello. Campiello (Esprit Long Term Research Project 25572) started in September 1997 and will last until August 2000. The main point in the Campiello approach is, that the community network access will be broadly extended into the real places through new interface metaphors (paper, large screen displays) and not only be accessible from Home PCs. This greater penetration and connection with real life should support a user base truly representative of the community and possibly open the community to more interactions with external members.
Assessment of glycosaminoglycan concentration changes in the intervertebral disc via chemical exchange saturation transfer (2011)

In this study, it is shown that the chemical exchange saturation transfer (CEST) method for hydroxyl protons can be used to detect changes in glycosaminoglycan (GAG) concentration in the intervertebral disc. The method, termed gagCEST, was demonstrated ex vivo by correlating the CEST effect with the fixed charge density (FCD) of the nucleus pulposus (NP), as well as by correlating tissue CEST images with their corresponding (23)Na images. Incubation of five NP samples with trypsin produced samples with varying GAG content (n = 19). A good correlation was found between the -OH CEST effect and FCD, as well as with the N-acetyl signal amplitude. gagCEST images in vitro further illustrated the amount of detail obtainable from this contrast mechanism when compared with conventional imaging. The large concentration of GAG and the relatively long T(1) of water in NP make the method sensitive, in particular, for the assessment of GAG depletion in this tissue. It is the loss of GAG in NP that indicates the early stage of disc degeneration.
The Novel Hydroxylamine Derivative NG-094 Suppresses Polyglutamine Protein Toxicity in Caenorhabditis elegans (2011)

Aggregation-prone polyglutamine (polyQ) expansion proteins cause several neurodegenerative disorders, including Huntington disease. The pharmacological activation of cellular stress responses could be a new strategy to combat protein conformational diseases. Hydroxylamine derivatives act as co-inducers of heat-shock proteins (HSPs) and can enhance HSP expression in diseased cells, without significant adverse effects. Here, we used Caenorhabditis elegans expressing polyQ expansions with 35 glutamines fused to the yellow fluorescent protein (Q35-YFP) in body wall muscle cells as a model system to investigate the effects of treatment with a novel hydroxylamine derivative, NG-094, on the progression of polyQ diseases. NG-094 significantly ameliorated polyQ-mediated animal paralysis, reduced the number of Q35-YFP aggregates and delayed polyQ-dependent acceleration of aging. Micromolar concentrations of NG-094 in animal tissues with only marginal effects on the nematode fitness sufficed to confer protection against polyQ proteotoxicity, even when the drug was administered after disease onset. NG-094 did not reduce insulin/insulin-like growth factor 1-like signaling, but conferred cytoprotection by a mechanism involving the heat-shock transcription factor HSF-1 that potentiated the expression of stress-inducible HSPs. NG-094 is thus a promising candidate for tests on mammalian models of polyQ and other protein conformational diseases.
Enhanced tumor binding using immunohistochemical analyses by second generation anti-tumor-associated glycoprotein 72 monoclonal antibodies versus monoclonal antibody B72.3 in human tissue. (1990)

Monoclonal antibody (MAb) B72.3 was generated using a membrane-enriched fraction of a human mammary carcinoma biopsy. It has demonstrated reactivity to the majority of human adenocarcinomas including colorectal, gastric, pancreatic, ovarian, endometrial, mammary, and nonsmall cell lung cancer as well as weak or nondetectable reactivity to the majority of normal adult tissues, with the exception of secretory endometrium. Radiolabeled B72.3 has demonstrated MAb localization of carcinoma in approximately 70% of several hundred colorectal and ovarian carcinoma patients. The B72.3-reactive antigen, tumor-associated glycoprotein 72, has been purified from a human colon cancer xenograft and used as an immunogen to generate second generation MAbs. Twenty-eight of these MAbs, designated CC (colon cancer), were shown to be reactive with tumor-associated glycoprotein 72; direct-binding radioimmunoassays, Western blotting, live cell surface binding assays, liquid competition radioimmunoassays, and affinity constant measurements distinguished CC MAbs from each other and from B72.3. Two of these MAbs, CC49 and CC112, were selected for further immunohistochemical characterization. These MAbs were tested here against a spectrum of normal, benign, and malignant human adult tissues using the avidin-biotin-peroxidase technique, and their reactivity was compared with B72.3. Both CC MAbs were more reactive than B72.3 against a range of tumors. Extensive testing with MAbs CC49 and B72.3 using serial tissue sections demonstrated that both MAbs reacted similarly to most normal adult tissues with MAb CC49 reacting stronger to inflammatory colonic tissue. In 35 of 48 (72%) carcinoma biopsies of the gastrointestinal tract, ovary, breast, and lung in which one of the MAbs reacted to at least 20% of the cells, CC49 reacted to a greater percentage of carcinoma cells and/or tumor-associated mucin than B72.3. The reciprocal was observed in only 2% of the carcinomas. This study thus provides evidence that these second generation anti-tumor-associated glycoprotein MAbs may be more efficient than B72.3 in the further study of human carcinoma cell populations and in the diagnostic and therapeutic procedures presently being pursued with MAb B72.3.
Cytokine regulation of human monocyte differentiation in vitro: the tumor-cytotoxic phenotype induced by macrophage colony-stimulating factor is developmentally regulated by gamma-interferon. (1993)

In this study we continue our examination of the unique form of antibody-dependent antitumor cytotoxicity (ADCC) which develops when human monocytes are exposed to macrophage colony-stimulating factor (MCSF) in vitro. This form of ADCC is not present in fresh monocytes and emerges only as monocytes differentiate into monocyte-derived macrophages (MDM). We now report that the presence of gamma-interferon (IFN-gamma) during this period of differentiation markedly affects the development of MCSF-induced cytotoxicity. The addition of IFN-gamma on day 0 resulted in a pronounced dose-dependent inhibition of ADCC measured on day 7 (mean inhibition, 76 +/- 12%; range, 66-94%). MDM exposed to MCSF plus IFN-gamma required 3-10-fold higher effector to target cell ratios to achieve a level of cytotoxicity comparable to that of MDM cultured with MCSF alone. This inhibitory effect was attributable to an IFN-gamma-induced shift in the basic mechanism of target cell killing, away from the phagocytic form normally seen with MCSF and toward a significantly less effective extracellular form. In order to influence ADCC, IFN-gamma had to be present prior to the onset of differentiation; if it was added later it had progressively less effect, and if it was added after differentiation was complete it was inactive. The modulatory effects of IFN-gamma also extended to a number of other developmentally regulated attributes. MDM cultured with MCSF normally acquired a characteristic morphology and immunophenotype, re-entered the cell cycle, and changed functionally from accessory cells for mitogen-induced lymphocyte activation to suppressor cells. The presence of IFN-gamma prevented the emergence of each of these attributes. Despite these negative effects, MDM exposed to IFN-gamma remained viable, continued to bind and internalize MCSF, and displayed superior accessory cell function and oxidative burst activity. Taken together, our findings suggest that human monocytes are capable of following at least two distinct differentiation pathways in response to the local cytokine environment, with significant impact on the resultant macrophage phenotype in general and antitumor cytotoxicity in particular.
Quantized conductance in a heterostructurally defined Ga0.25In0.75As/InP quantum wire (1997)

We report on the observation of quantized conductance up to 10 K in epitaxially regrown, heterostructurally defined, 100-nm-wide Ga0.25In0.75As/InP quantum wires. In addition to the plateaus at integer steps of 2e2/h, we observe plateaus at 0.2(2e2/h), 0.7(2e2/h), and 1.5(2e2/h), indicating spin polarization at zero magnetic field. Of these, the first two plateaus appear to evolve into one at around 0.5(2e2/h) when the sample is subjected to a magnetic field parallel to the quantum wire. The observation of quantized conductance is made possible by the substantial improvement in the quality of the interface by regrowth.
Mutation spectrum of the rhodopsin gene among patients with autosomal dominant retinitis pigmentosa. (1991)

We searched for point mutations in every exon of the rhodopsin gene in 150 patients from separate families with autosomal dominant retinitis pigmentosa. Including the 4 mutations we reported previously, we found a total of 17 different mutations that correlate with the disease. Each of these mutations is a single-base substitution corresponding to a single amino acid substitution. Based on current models for the structure of rhodopsin, 3 of the 17 mutant amino acids are normally located on the cytoplasmic side of the protein, 6 in transmembrane domains, and 8 on the intradiscal side. Forty-three of the 150 patients (29%) carry 1 of these mutations, and no patient has more than 1 mutation. In every family with a mutation so far analyzed, the mutation cosegregates with the disease. We found one instance of a mutation in an affected patient that was absent in both unaffected parents (i.e., a new germ-line mutation), indicating that some "isolate" cases of retinitis pigmentosa carry a mutation of the rhodopsin gene.
Histopathologic Subsets of Fibrosing Alveolitis in Patients with Systemic Sclerosis and Their Relationship to Outcome (2002)

Fibrosing alveolitis associated with systemic sclerosis (FASSc) has a better prognosis than idiopathic pulmonary fibrosis. In view of recent reports that idiopathic nonspecific interstitial pneumonia (NSIP) has a better prognosis than idiopathic usual interstitial pneumonia (UIP), we classified histologic appearances of surgical lung biopsies performed in 80 patients with FASSc. NSIP (n = 62, 77.5%), subcategorized as cellular NSIP (n = 15) and fibrotic NSIP (n = 47) was much more prevalent than UIP (n = 6), end-stage lung disease (ESL, n = 6), or other patterns (n = 6). There were 25 deaths (NSIP 16/62, 26%; UIP/ESL 6/12, 50%). Five-year survival differed little between NSIP (91%) and UIP/ESL (82%); mortality was associated with lower initial carbon monoxide diffusing capacity (DL(CO)) and FVC levels (p = 0.004 and p = 0.007, respectively). Survival and serial FVC and DL(CO) trends did not differ between cellular and fibrotic NSIP. Increased mortality in NSIP was associated with lower initial DL(CO) levels (p = 0.04), higher BAL eosinophil levels (p = 0.03), and deterioration in DL(CO) levels during the next 3 years (p < 0.005). We conclude that NSIP is the histopathologic pattern in most patients with FASSc. However, outcome is linked more strongly to disease severity at presentation and serial DL(CO) trends than to histopathologic findings.
Indirect Reduction and Percutaneous Screw Fixation of Displaced Tibial Plateau Fractures (1992)

Summary Indirect reduction and percutaneous screw fixation were attempted in 20 displaced tibial plateau fractures in 20 patients. Closed, indirect reduction was successful in 18 fractures; two others, both Schatzker type II fractures, required open reduction. The 18 fractures were followed for an average of 16.2 months (range, 12–24 months). Of the fractures successfully reduced with indirect techniques, 13 were reduced anatomically (72.2%), and five were considered nonanatomic (27.8%). Four of the five fractures with a nonanatomic reduction were type II fractures. Clinically, there were six excellent (33%), 10 good (56%), and two fair (11%) results. No fracture lost reduction; no patient developed an infection. Indirect techniques could effectively reduce only split fragments. Depressed fragments could not be reduced reliably with either lig-amentotaxis or percutaneous elevation with a tamp. There was no correlation between radiographic reduction and clinical outcome. It did not matter whether two, three, or four screws were used to stabilize the fracture.
Novel dual-frequency and broad-band designs of slot-loaded equilateral triangular microstrip antennas (2000)

By loading properly arranged slots in an equilateral-triangular microstrip patch, novel dual-frequency and broad-band operations of a single-feed triangular microstrip antenna are presented. For dual-frequency operation, the proposed design is achieved by loading two pairs of narrow slots in the triangular patch, one embedded close to the side edges of the patch and the other inserted at the bottom edge of the patch. The obtained two operating frequencies are of same polarization planes and by varying the positions and lengths of the inserted slots at the bottom edge of the patch, a tunable frequency ratio of the two frequencies ranging from about 1.16 to 2.06 is obtained. Furthermore, it is found that by protruding a narrow slot out of the embedded slots close to the side edges, broad-band operation of the triangular microstrip antenna near its fundamental resonant mode can be achieved. Results show that the antenna bandwidth of the proposed broad-band design can be greater than 2.6 times that of a conventional triangular microstrip antenna. Details of the proposed dual-frequency and broad-band designs are described and typical experimental results are presented and discussed.
Next Generation Wireless LANs (2013)

If you've been searching for a way to get up to speed on IEEE 802.11n and 802.11ac WLAN standards without having to wade through the entire specification, then look no further. This comprehensive overview describes the underlying principles, implementation details and key enhancing features of 802.11n and 802.11ac. For many of these features the authors outline the motivation and history behind their adoption into the standard. A detailed discussion of key throughput, robustness, and reliability enhancing features (such as MIMO, multi-user MIMO, 40/80/160 MHz channels, transmit beamforming and packet aggregation) is given, plus clear summaries of issues surrounding legacy interoperability and coexistence. Now updated and significantly revised, this 2nd edition contains new material on 802.11ac throughput, including revised chapters on MAC and interoperability, plus new chapters on 802.11ac PHY and multi-user MIMO. An ideal reference for designers of WLAN equipment, network managers, and researchers in the field of wireless communications.
Real-time optical waveform convolver/cross correlator (1984)

We show that an inhomogeneously broadened sample, excited by a series of three resonant optical pulses, emits an optical signal whose electric field envelope closely approximates the convolution or cross correlation of the field envelopes belonging to two of the three excitation pulses. The convolution (cross correlation) function is obtained when the first (second) pulse in the excitation sequence is short compared to the temporal structure of the other two excitation pulses.
Myelodysplastic Syndromes (2002)

The myelodysplastic syndromes (MDS) are characterized by hemopoietic insufficiency associated with cytopenias leading to serious morbidity plus the additional risk of leukemic transformation. Therapeutic dilemmas exist in MDS because of the disease's multifactorial pathogenetic features, heterogeneous stages, and the patients' generally elderly ages. Underlying the cytopenias and evolutionary potential in MDS are innate stem cell lesions, cellular/cytokine-mediated stromal defects, and immunologic derangements. This article reviews the developing understanding of biologic and molecular lesions in MDS and recently available biospecific drugs that are potentially capable of abrogating these abnormalities. Dr. Peter Greenberg's discussion centers on decision-making approaches for these therapeutic options, considering the patient's clinical factors and risk-based prognostic category. One mechanism underlying the marrow failure present in a portion of MDS patients is immunologic attack on the hemopoietic stem cells. Considerable overlap exists between aplastic anemia, paroxysmal nocturnal hemoglobinuria, and subsets of MDS. Common or intersecting pathophysiologic mechanisms appear to underlie hemopoietic cell destruction and genetic instability, which are characteristic of these diseases. Treatment results and new therapeutic strategies using immune modulation, as well as the role of the immune system in possible mechanisms responsible for genetic instability in MDS, will be the subject of discussion by Dr. Neal Young. A common morphological change found within MDS marrow cells, most sensitively demonstrated by electron microscopy, is the presence of ringed sideroblasts. Such assessment shows that this abnormal mitochondrial iron accumulation is not confined to the refractory anemia with ring sideroblast (RARS) subtype of MDS and may also contribute to numerous underlying MDS pathophysiological processes. Generation of abnormal sideroblast formation appears to be due to malfunction of the mitochondrial respiratory chain, attributable to mutations of mitochondrial DNA, to which aged individuals are most vulnerable. Such dysfunction leads to accumulation of toxic ferric iron in the mitochondrial matrix. Understanding the broad biologic consequences of these derangements is the focus of the discussion by Dr. Norbert Gattermann.
<title>Ocular partial-coherence interferometry</title> (1996)

In the past interferometry has been applied in three main fields. Fringe interferometry has been used for the topographic measurement of surfaces. Interferometric length measurement techniques have been used for the measurement of distances, refractive indices, and wavelengths. In addition interferometric techniques have also been used in synthetic aperture imaging. Here we discuss various interferometric techniques which can be used in ophthalmology, describe the problems involved and present the results obtained so far.
Pattern Classification (2nd ed.) (1999)

NOT FOR GENERAL DISTRIBUTION; for use only by students of designated faculty. This is a pre-publication print of material to appear in Duda, Hart and Stork: Pattern Classification and Scene Analysis: Part I Pattern Classification, to be published in 1998 by John Wiley &amp;amp; Sons, Inc. This is a preliminary version and may contain errors; comments and suggestions are heartily encouraged.