gebhart/patrepeval huggingface dataset:
Expression of alpha v beta 5 integrin is necessary for efficient adenovirus-mediated gene transfer in the human airway (1995)

Recombinant adenoviruses are being evaluated for gene therapy of cystic fibrosis lung disease with the goal of reconstituting the expression of the cystic fibrosis transmembrane conductance regulator in pulmonary epithelia by direct administration of the virus into the airway. The therapeutic potential of recombinant adenoviruses is limited in part by the relative inefficiency by which gene transfer occurs. This study uses a human bronchial xenograft model to study adenovirus infection in the human airway in an attempt to define the molecular events that limit gene transfer. Our studies of the human airway confirm previous observations of cell lines that have indicated a two-step process for adenovirus entry, which begins with the binding of the virus to the cell through the fiber protein and continues with internalization via interactions among cellular integrins and an RGD motif (Arg-Gly-Asp) in the penton base. Furthermore, the level of maturity of the epithelia in xenografts has a major impact on gene transfer. Undifferentiated epithelia express high levels of alpha v beta 5 integrins and are easily infected with recombinant adenoviruses; gene transfer is completely inhibited with excess fiber and partially inhibited with RGD peptide and alpha v beta 5 integrin antibody. Pseudostratified epithelia do not express alpha v beta 5 integrin in differentiated columnar cells and are relatively resistant to adenovirus-mediated gene transfer; what little gene transfer occurs is inhibited by fiber but not by RGD peptide or alpha v beta 5 integrin antibody. These studies suggest that the expression of integrins in human airway epithelia limits the efficiency of gene transfer with recombinant adenoviruses. However, low-level gene transfer can occur in fully mature epithelia through alpha v beta 5 integrin-independent pathways.
Heparan Sulfate Proteoglycan Mimetics Promote Tissue Regeneration: An Overview (2012)

Once the bleeding is controlled, inflammatory cells migrate into the wound area. This is the start of the inflammatory phase, which is characterized by the sequential infiltration of neutrophils, macrophages, and lymphocytes
Electrolytic capacitor elimination in power electronic system by high frequency active filter (2002)

The application of a high frequency current-fed type active filter for elimination of electrolytic capacitor in the DC link of an inverter is described. The active filter operation has been described, preliminary analysis and design have been made, and simulation studies have been made in detail for a three-phase inverter in PWM and square-wave modes and a single-phase inverter in PWM mode to prove the feasibility of concepts. The study indicates that it is possible to eliminate the unreliable electrolytic capacitor by high frequency active filter.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
In Vitro and in Vivo Evaluation of 64Cu-Labeled DOTA−Linker−Bombesin(7−14) Analogues Containing Different Amino Acid Linker Moieties (2007)

The gastrin-releasing peptide receptor (GRPR) is overexpressed on a variety of tumor types and has been targeted with radiolabeled peptides for detection and therapy of these cancers. Analogues of the 14 amino acid bombesin (BN) peptide have been radiolabeled with both gamma- and positron-emitting radionuclides for detection of GRPR-expressing tumors. We have previously evaluated BN analogues radiolabeled with the positron-emitter, copper-64 (64Cu), that contained various aliphatic linkers placed between the BN peptide and the 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelator. These studies showed that the analogues could be used for positron-emission tomographic (PET) imaging of GRPR-positive tumors in mice but clinical translation would be hindered by significant uptake in background tissues. Therefore, the purpose of this study was to determine if the use of amino acid linkers placed between the DOTA chelate and the BN peptide would reduce nontarget tissue uptake, while maintaining good prostate tumor uptake. The linkers studied utilized three amino acid combinations of glycine (G), serine (S), or glutamic acid (E). In vitro assays in PC-3 cells showed that the glutamic acid-containing linkers had poor binding and internalization, while the other analogues had IC50 values <100 nM and good internalization. In vivo, these same analogues demonstrated tumor-specific uptake and good imaging characteristics that were comparable to, or better than the previously reported 64Cu-labeled DOTA-BN analogues. Overall, this study shows that BN analogues containing amino acid linkers can be used for the PET imaging of GRPR-expressing prostate cancer and that these linkers lead to lower background tissue uptake.
Patient-Specific Induced Pluripotent Stem-Cell Models for Long-QT Syndrome (2010)

We generated patient-specific pluripotent stem cells from members of a family affected by long-QT syndrome type 1 and induced them to differentiate into functional cardiac myocytes. The patient-derived cells recapitulated the electrophysiological features of the disorder. (Funded by the European Research Council and others.)
Inhibition of Connective Tissue Growth Factor (CTGF/CCN2) Expression Decreases the Survival and Myogenic Differentiation of Human Rhabdomyosarcoma Cells (2004)

Connective tissue growth factor (CTGF/CCN2), a cysteine-rich protein of the CCN (Cyr61, CTGF, Nov) family of genes, emerged from a microarray screen of genes expressed by human rhabdomyosarcoma cells. Rhabdomyosarcoma is a soft tissue sarcoma of childhood deriving from skeletal muscle cells. In this study, we investigated the role of CTGF in rhabdomyosarcoma. Human rhabdomyosarcoma cells of the embryonal (RD/12, RD/18, CCA) and the alveolar histotype (RMZ-RC2, SJ-RH4, SJ-RH30), rhabdomyosarcoma tumor specimens, and normal skeletal muscle cells expressed CTGF. To determine the function of CTGF, we treated rhabdomyosarcoma cells with a CTGF antisense oligonucleotide or with a CTGF small interfering RNA (siRNA). Both treatments inhibited rhabdomyosarcoma cell growth, suggesting the existence of a new autocrine loop based on CTGF. CTGF antisense oligonucleotide-mediated growth inhibition was specifically due to a significant increase in apoptosis, whereas cell proliferation was unchanged. CTGF antisense oligonucleotide induced a strong decrease in the level of myogenic differentiation of rhabdomyosarcoma cells, whereas the addition of recombinant CTGF significantly increased the proportion of myosin-positive cells. CTGF emerges as a survival and differentiation factor and could be a new therapeutic target in human rhabdomyosarcoma.
Taurolidine 2% as an Antimicrobial Lock Solution for Prevention of Recurrent Catheter‐Related Bloodstream Infections (1998)

These data support previous observations made outside North America and suggest that taurolidine may prove to be an effective and safe antimicrobial agent for the prevention of recurrent catheter-related bloodstream infections.
The influence of triamcinolone acetonide on joint stiffness in the rat. (1971)

Triamcinolone acetonide under the conditions of this experiment greatly diminished or inhibited the occurrence of stiffness in normal rat joints immobilized three weeks.
The animals had evidence of sonic systemic effect with the dosage utilized.
Structure and Function of the DNA Repair Enzyme Exonuclease III from E. Colia (1994)

The three-dimensional structure of exonuclease III, the major AP DNA repair endonuclease of Escherichia coli, has been determined using x-ray crystallographic methods at 2.7 A resolution. The atomic model was fit to an electron density map calculated with phases obtained from three isomorphous heavy atom derivatives. The overall chain fold of exonuclease III is that of a compact alpha,beta-protein of dimensions 55 by 50 by 45 A. The pair of extended beta-pleated sheets pack against each other in an approximately parallel fashion to form the hydrophobic core of a four-layered sandwich structure. These beta sheets are flanked by four alpha-helices that form the outer two layers of the fold. The individual strands of the beta-sheets are in a mostly antiparallel configuration and are linked by extensive loop regions that connect adjoining strands. The structure contains internal symmetry with the two extended beta-sheets and four alpha-helices related by a pseudo-twofold axis running approximately down the center of the two sheets. This internal symmetry is not mirrored in the structure of the loop regions, nor is it detectable within the amino acid sequence. There is a "groove" between the beta-sheets at one end of the molecule that is bordered by several of the exposed loop regions and may be significant for DNA binding.
NEWS OF THE WEEK (2005)

RETURN TO ISSUEPREVNewsNEXTNEWS OF THE WEEKBASF BUYS MERCK CHEMICAL UNITBASF to strengthen its hand in the electronic chemicals sectorALEX TULLOCite this: Chem. Eng. News 2005, 83, 6, 8Publication Date (Print):February 7, 2005Publication History Published online14 November 2010Published inissue 7 February 2005https://pubs.acs.org/doi/10.1021/cen-v083n006.p008https://doi.org/10.1021/cen-v083n006.p008newsACS PublicationsCopyright © 2005 American Chemical SocietyArticle Views9Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Manufacturing Get e-Alerts
Strategies for Developing Biomarkers of Heart Failure (2004)

Strategically, biomarkers linked to mechanisms involved in the etiology of HF, such as dysregulation of ion transport, seem best suited for serving as early biological markers to predict and diagnose disease, select therapy, or assess progression.
A rapid and simple method for inactivating chromosomal genes inYersinia (2003)

A polymerase chain reaction (PCR)-based procedure without any cloning step was developed for a rapid mutagenesis/deletion of chromosomal target genes in Yersinia. For this purpose, a PCR fragment carrying an antibiotic resistance gene flanked by regions homologous to the target locus is electroporated into a recipient strain expressing the highly proficient homologous recombination system encoded by plasmid pKOBEG-sacB. Two PCR procedures were tested to generate an amplification product formed of an antibiotic resistance gene flanked by short (55 bp) or long (500 bp) homology extensions. Using this method, three chromosomal loci were successfully disrupted in Yersinia pseudotuberculosis. The use of this technique allows rapid and efficient large-scale mutagenesis of Yersinia target chromosomal genes.
Oligomerization and cyclization reactions of acetylene (2004)

Acetylene was pyrolyzed in a flow system at pressures between 104 and 403 Torr and temperatures between 854 and 971 K. Products were analyzed by gas chromatography with flame ionization and mass spectrometric detection. In some experiments the reactor was placed in the chromatographic oven to limit condensation. Twenty-four products were detected, of which fifteen were found to be primary. Orders and Arrhenius parameters were found for thirteen products. The results were interpreted in terms of a radical mechanism with 24 reactions for pure acetylene. Radical addition to acetylene produced chemically activated radicals, which could either eliminate a hydrogen atom or add more acetylene. If the radical contained five or more atoms, cyclization to form cyclopentadiene or an aromatic compound, respectively, was also possible. It was suggested that radical recombination could produce chemically activated intermediates that could cyclize to form cyclohexadienes. Addition of neopentane introduced methyl radicals, which could participate in another series of ten chemically activated acetylene addition and hydrogen elimination reactions to form propyne, cyclopentadiene and toluene. Five quotients of rate constants were calculated for competitive reactions.
EFFECT OF AN INHIBITOR OF GLUTAMINE SYNTHESIS (METHIONINE SULFOXIMINE) ON AMMONIA TOXICITY AND METABOLISM. (1964)

Abstract Glutamine formation is the major pathway of ammonia detoxication in tissues other than the liver. When a competitive inhibitor of the glutamine synthetase reaction, methionine sulfoximine, was administered to mice, there was, however, a marked decrease in in vivo ammonia toxicity. The peak brain ammonia concentration following the injection of the LD 50 for untreated mice was actually significantly higher in the methionine sulfoximine-treated mice because of an increase in the base-line brain ammonia concentration, and yet there were no deaths in the treated group. Methionine sulfoximine had an effect on endogenous ammonia metabolism, as evidenced by a doubling of the brain ammonia concentrations 2 hours after its administration that lasted for at least 24 hours. This inhibitor of the glutamine synthetase reaction also interfered with the detoxication of an exogenous dose of ammonia and the formation of glutamine from this ammonia load. These studies indicate that ammonia intoxication does not depend upon the mere presence of high cerebral ammonia levels, but is related to the so-called mechanism of detoxication by which ammonia enters into the cerebral metabolic cycles.
Sparse Modeling for Image and Vision Processing (2014)

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci-entific research documents, whether they are pub-lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et a ̀ la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Clinical and Immunologic Effects of Subcutaneously Administered Interleukin-12 and Interferon Alfa-2b: Phase I Trial of Patients With Metastatic Renal Cell Carcinoma or Malignant Melanoma (2004)

Concurrent SC administration of IL-12 and IFN-alpha-2b is possible at the dose levels utilized. Recommended doses for phase II trials are 500 ng/kg IL-12 and 1.0 MU/m(2) IFN-alpha-2b. Consistent induction of IP-10 and Mig, as well as variable induction of B7.1, interleukin-5, and interferon gamma expression was noted in PBMCs.
Comparison of effect of nicotine and levamisole and ivermectin on mortality of leech (2014)

To study the effect of different doses of nicotine on Limnatis in comparison with levamisole and ivermectin. In this interventional experimental study in July 2012, the amount of 61 mature leeches of Limnatis nilotica species were collected and anti-parasitic effects of drug treatments using anti-leech method were assessed. So that, a leech was placed in the dishes with 600 mL spring water and leech's paralysis and death time were recorded accurately for 720 min. A total of 9 replicates were considered for each treatment. Six drug treatments were considered. Pharmacological treatments include nicotine (5, 10 and 20 mg doses), levamisole (10 mg), ivermectin (10 mg) and distilled water. Data were analyzed using the Sigma ASA 2 software and pair t-test method with less than 0.05 confidence levels. The results of present study show that doses of 5, 10 and 20 mg of nicotine, with time average of 2.44±0.52, 1.88±0.78 and 1.55±0.72 min cause to death of leeches. Ivermectin and levamisole cause to death of leeches, averaging 7.44±1.12 and 14.66±5.09 min, respectively. Distilled water treatment has been reported as an ineffective group. Data analysis showed that the group receiving 5 mg nicotine, had minimum time of death and there are statistical differences among all groups (P>0.05), but there are not significant differences between treatments receiving 10 mg nicotine, with 5 and 20 mg nicotine treatments. It appears that nicotine compound as the effective substance of tobacco plant has the strong anti-leech effect on Limnatis nilotica species and can be used as leech purposes in the future.
Water‐Soluble Ionic Liquids as Novel Stabilizers in Suspension Polymerization Reactions: Engineering Polymer Beads (2006)

Aqueous solutions of ionic liquids have been used as novel and environmentally friendly reaction media to synthesize and "control" the size of different cross-linked polymer beads by suspension polymerization reactions. It was found that the investigated ionic liquids can act as novel stabilizing agents of the suspensions as a result of their surface-active properties. The results have demonstrated that the average size of polymer beads can be varied from the macro- to the nanoscale and their surface area can also be "adjusted" by this synthetic approach. Furthermore, the use of a combination of ionic liquids and water for the synthesis of polymers, the simple isolation of the products formed in this polymerization procedure, as well as the recycling of the continuous medium for further reactions open up possibilities for the development of "new and green" polymerization processes.
Genetic improvement of seed storage proteins using three variant alleles of 7S globulin subunits in soybean(Glycine max L.). (1989)

Genetrcally fixed "7S-low lines" were developed from a cross Keburi × F1 (Oodate No. 1 × Mo-shi-dou Gong 503) in soybean. Seven S-low lines are characterized by the lack of the α'-subunit of 7S globulin and a marked decrease of both the α- and β-subunits of 7S globulin due to the simultaneous incorporation of α'-null, α-low and β-low alleles. The 7S-low lines and ordinary varieties were examined for their respective contents of 7 S and 11S globulins, total protein contents, development of protein bodies and amino acid composition in the mature seeds. The contents of 7S globulin in the 7S-low lines were only half of those in the ordlnary varieties, whereas the contents of 11S globulin in the lines were about 14% higher than those in the ordinary varieties. The marked modifications in the protein compositlon of the seeds did not adversely affect the total protein contents as well as de-velopment and morphology of the protein bodies. A high negative correlation was found be-tween the 7S and 11S globulin contents (r= -0.84, P{r=0}0.001), and the calculated absolute value of the slope of the regression line was slightly larger than 1.0 (a=-1.5). These results suggest that 11S globulin may compensate for the decrease in 7S globulin to maintain the production of total seed protein in the 7S-low lines. The mean value of the sulfur-containing amino acid contents in the seeds of four representative 7S-low lines was about 20% higher than that of four ordinary varieties.
Willful Blindness (2015)

Lack of unity among nurses and a weak position on the clinical power ladder are still marring this profession. So are the 12-hour shifts Maureen Shawn Kennedy mentions in her editorial. I have worked eight-hour shifts and 12-hour shifts on a transplant unit. After an eight-hour shift, I was able to go home and plan other activities for the day and be present in my personal relationships. By contrast, after a 12-hour shift, I felt so fatigued that I needed to go to sleep immediately. In time, 12-hour shifts take a toll on personal relationships, as well as on a nurse's performance and mental acuity. Liliana Martin, RN Cedar Park, TX
Comparative proteomic profiling of the serum differentiates pancreatic cancer from chronic pancreatitis (2017)

Finland ranks sixth among the countries having highest incidence rate of pancreatic cancer with mortality roughly equaling incidence. The average age of diagnosis for pancreatic cancer is 69 years in Nordic males, whereas the average age of diagnosis of chronic pancreatitis is 40-50 years, however, many cases overlap in age. By radiology, the evaluation of a pancreatic mass, that is, the differential diagnosis between chronic pancreatitis and pancreatic cancer is often difficult. Preoperative needle biopsies are difficult to obtain and are demanding to interpret. New blood based biomarkers are needed. The accuracy of the only established biomarker for pancreatic cancer, CA 19-9 is rather poor in differentiating between benign and malignant mass of the pancreas. In this study, we have performed mass spectrometry analysis (High Definition MS<sup>E</sup> ) of serum samples from patients with chronic pancreatitis (13) and pancreatic cancer (22). We have quantified 291 proteins and performed detailed statistical analysis such as principal component analysis, orthogonal partial least square discriminant analysis and receiver operating curve analysis. The proteomic signature of chronic pancreatitis versus pancreatic cancer samples was able to separate the two groups by multiple statistical techniques. Some of the enriched pathways in the proteomic dataset were LXR/RXR activation, complement and coagulation systems and inflammatory response. We propose that multiple high-confidence biomarker candidates in our pilot study including Inter-alpha-trypsin inhibitor heavy chain H2 (Area under the curve, AUC: 0.947), protein AMBP (AUC: 0.951) and prothrombin (AUC: 0.917), which should be further evaluated in larger patient series as potential new biomarkers for differential diagnosis.
Lipooligosaccharides: The Principal Glycolipids of the Neisserial Outer Membrane (1988)

The outer-membrane glycolipids of bacteria that colonize mucosal surfaces that are not routinely bathed by bile acids often lack the long, hydrophilic and neutral polysaccharides that protect the lipid membranes of enteric bacteria from dispersal. The glycolipid from these organisms is properly termed a lipooligosaccharide. A Neisseria strain makes from two to six lipooligosaccharide molecules that range in Mr from 3,150 to 7,100. Different species of Neisseria commonly make lipooligosaccharides of identical Mr and epitope content. Differences in oligosaccharides account for most of the observed physical heterogeneity. Oligosaccharides consist of (1) partially conserved and highly substituted basal oligosaccharides that branch at heptose residues; (2) a linear segment consisting of (hexose)n residues that determines the length of the oligosaccharide; and (3) terminal sequences that are similar to those of glycosphingolipids. Epitope expression is linked to physical heterogeneity and is modified by the molecular environment of the outer membrane. Serotype epitopes are present only on lipooligosaccharides of a certain Mr. Certain lipooligosaccharides regulate complement activation onto the bacterial surface and, hence, immune lysis.