gebhart/patrepeval huggingface dataset:
Randomization of autogenous vein and polytetrafluorethylene grafts in femoral-distal reconstruction. (1982)

A randomized, prospective, controlled study comparing autogenous saphenous vein and polytetrafluoroethylene (PTFE) grafts in infrainguinal arterial reconstructions has been initiated in three different institutions. A total of 446 operative procedures were divided into six groups, depending on the site of distal bypass insertion into the popliteal or an infrapopliteal artery and on whether the patient received a randomized vein or PTFE graft or an obligatory PTFE graft. The randomized saphenous vein graft patency to infrapopliteal arteries was significantly better (P less than 0.005) at 2 1/2 years than the patency of randomized or obligatory PTFE grafts to the same level. No significant differences between randomized vein grafts and randomized or obligatory PTFE grafts in the femoropopliteal position could be demonstrated up to 2 1/2 years, either above or below the knee. The need for such a study and the limitations of this preliminary report are discussed. The requirement for longer observation of greater numbers of cases is stressed. A current plan for usage of the PTFE graft in the lower extremity is presented.
High solder joint reliability with lead free solders (2004)

Recently. preventing environmental pollutions. lead-free (Pb-free) soldcrs are about to replace tin-lead (Sn-Pbj eutectic solders. Sn-Ag-Cu alloys arc lcading candidatcs for lead free solders. However. Sn-Ag-Cu alloys were not satisfied to meet sever custoiner requirements. At first, optimum silver wt%
Neuroprotective role of melatonin in oxidative stress vulnerable brain. (2003)

The brain is deficient in oxidative defense mechanisms and hence is at greater risk of damage mediated by reactive oxygen species (ROS) resulting in molecular and cellular dysfunction. Emerging evidence suggesting the activation of glutamate gated cation channels, may be another source of oxidative stress, leading to neuronal degeneration. Oxidative stress has been implicated in the development of neurodegenerative diseases like Parkinsonism, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, epileptic seizures, and stroke. Melatonin, the pineal hormone, acts as a direct free radical scavenger and indirect antioxidant. It is suggested that the increase in neurodegenerative diseases is attributable to a decrease in the levels of melatonin with age. Melatonin has been shown to either stimulate gene expression for the antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) or to increase their activity. Additionally, it neutralizes hydoxyl radical, superoxide radical, peroxyl radical, peroxynitrite anion, singlet oxygen, hydrogen peroxide, nitric oxide, and hypochlorous acid. Unlike other antioxidants, melatonin can easily cross all morphophysiological barriers, e.g., the blood brain barrier, and enters cells and subcellular compartments. Though evidence are accumulating to suggest the potential of melatonin in neurodegenerative conditions, much information needs to be generated before the drug can find place in neurology clinics.
Biochemistry and genetics of mannan-binding lectin (MBL) (2003)

Mannose- or mannan-binding lectin (MBL) is a member of the collectin protein family, which includes lung surfactant proteins SP-A and SP-D. Each member consists of similar or identical polypeptide chains with a region of collagen-like sequence followed by a C-type lectin domain. The polypeptides associate in threes to form a subunit containing a collagen-like helix, with three clustered lectin domains. These subunits associate into larger structures, usually with 12-18 polypeptides. The collectins bind to patterns of neutral sugars on surfaces (e.g. of micro-organisms) and mediate effector functions associated with killing/phagocytosis. MBL is the only collectin which activates complement. It resembles in quaternary structure the complement protein C1q, which recognizes targets via charge clusters. Binding of MBL to a surface activates MBL-associated serine proteases (MASPs) attached to MBL, and MASP-2 activates complement proteins C4 and C2. The MASPs are homologous to the C1q-associated proteases, C1r and C1s. MBL therefore activates complement by a mechanism very similar to C1q, and engages the opsonic activity of complement to clear micro-organisms. The serum concentration of MBL is very variable in humans. The variability is largely associated with mutations leading to amino acid substitutions in the collagen-like region which decrease MBL assembly and stability. Many studies demonstrate that MBL deficiency is associated with susceptibility to a range of infectious and inflammatory diseases.
ADOPTIVE TRANSFER OF EBV SPECIFIC T-CELLS (EBV-CTL) FOR TREATMENT OF DOCUMENTED EBV LYMPHOMA FOLLOWING ALLOGENEIC HEMATOPOIETIC STEM CELL (HSCT) OR ORGAN TRANSPLANTS (OT): CLINICAL, VIRAL AND IMMUNOLOGIC CORRELATES. (2010)

Doubrovina, E.1; Oflaz-Sozmen, B.1; Kernan, N.1; Young, J.2; Abramson, S.3; Barker, J.2; Boulad, F.3; Castro-Malaspina, H.2; Teruya-Feldstein, J.3; Filippa, D.3; Jakubowski, A.2; Kanaeva, E.3; Papadopoulos, E.2; Prockop, S.3; Scaradavou, A.3; Small, T.3; O'Reilly, R.3 Author Information
Localization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptides (1984)

The binding sites for four monoclonal antibodies, rho 1D4, rho 3C2, rho 3A6, and rho 1C5, have been localized within the C-terminal region of bovine rhodopsin: Asp18'-Glu-Ala16'-Ser-Thr-Thr-Val12'-Ser-Lys-Thr-Gl u8'-Thr-Ser-Gln-Val4'-Ala-Pr o -Ala1'. Antibody binding sites were localized by using synthetic C-terminal peptides in conjunction with solid-phase competitive inhibition assays and limited proteolytic digestion of rhodopsin in conjunction with electrophoretic immunoblotting techniques. Binding of the rho 1D4 and rho 3C2 antibodies to immobilized rhodopsin was inhibited with peptides of length 1'-8' and longer. Antibody rho 1D4 binding was not inhibited by peptides 2'-13' or 3'-18', indicating that the C-terminal alanine residue of rhodopsin was required. Similar competitive inhibition studies indicated that the antibody rho 3A6 required peptides of length 1'-12' and longer whereas rho 1C5 required peptide 1'-18'. Peptide 3'-18' was as effective as 1'-18' in inhibiting rho 3A6 binding to rhodopsin, but replacement of glutamic acid in position 8' with glutamine abolished competition. This substitution had little effect on the binding of antibody rho 1C5. Thus, Glu8' was essential for rho 3A6 binding but not for the binding of the rho 1C5 antibody. Cleavage of the seven amino acid C-terminus from rhodopsin and further cleavage to F1 (Mr 25 000) and F2 (Mr 12 000) fragments with Staphylococcus aureus V8 protease abolished binding of rho 1D4 antibody to the membrane-bound rhodopsin fragments.(ABSTRACT TRUNCATED AT 250 WORDS)
Essential Role of AKT-1/Protein Kinase Bα in PTEN-Controlled Tumorigenesis (2002)

PTEN is mutated at high frequency in many primary human cancers and several familial cancer predisposition disorders. Activation of AKT is a common event in tumors in which the PTEN gene has been inactivated. We previously showed that deletion of the murine Pten gene in embryonic stem (ES) cells led to increased phosphatidylinositol triphosphate (PIP(3)) accumulation, enhanced entry into S phase, and better cell survival. Since PIP(3) controls multiple signaling molecules, it was not clear to what degree the observed phenotypes were due to deregulated AKT activity. In this study, we mutated Akt-1 in Pten(-/-) ES cells to directly assess the role of AKT-1 in PTEN-controlled cellular processes, such as cell proliferation, cell survival, and tumorigenesis in nude mice. We showed that AKT-1 is one of the major downstream effectors of PTEN in ES cells and that activation of AKT-1 is required for both the cell survival and cell proliferation phenotypes observed in Pten(-/-) ES cells. Deletion of Akt-1 partially reverses the aggressive growth of Pten(-/-) ES cells in vivo, suggesting that AKT-1 plays an essential role in PTEN-controlled tumorigenesis.
The Use of Peptide Library for the Determination of Kinase Peptide Substrates (2003)

Protein phosphorylation plays a crucial role in regulating a plethora of intracellular biological activities. Because protein phosphorylation is a reversible reaction, it allows living cells to reset to the basal state rapidly after stimulation. There are hundreds of protein kinases involved in this general signaling machinery. These kinases are classified into three different categories based on their abilities to phosphorylate serine/threonine, or tyrosine, or both residues A comparison of primary sequences of protein kinases has indicated that the catalytic site (the kinase domain) is highly conserved, suggesting a common ancestor for these kinases (1) However, different kinases have evolved to function distinctly in response to diverse cellular stimuli. The specificities of protein kinases has thus become a critical issue in understanding signal transduction.
The embryoid body as a novel in vitro assay system for antiangiogenic agents. (1998)

Tumor progression necessitates the induction of blood vessels that converge upon the tumor and enhance the diffusibility of oxygen and nutrients. Approaches to treat cancer by antiangiogenic therapy are therefore straightforward, and there is a great need for suitable in vitro systems to test antiangiogenic agents. In the present study, embryoid bodies (EBs) differentiated from totipotent mouse embryonic stem (ES) cells and cultivated using the spinner flask technique are introduced as an in vitro system for antiangiogenesis research. ES cells effectively differentiated endothelial cells within the three-dimensional tissue of EBs. The total area of capillary-like structures, which were positive for CD31 (platelet endothelial cell adhesion molecule, PECAM-1), was assessed by confocal laser scanning microscopy and image analysis of a series of optical sections. Endothelial differentiation occurred between Day 4-5 and Day 8 of EB development. Within 7 days, 100% of EBs contained capillary-like structures. Suramin, tamoxifen, tetrahydrocortisol, and a combination of tetrahydrocortisol and heparin were tested for their antiangiogenic capacity in the EB system and were found to efficiently inhibit endothelial differentiation. Diffusion studies of a 10-kd 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF)-dextran and the fluorescent, amphiphilic agent doxorubicin in avascular and vascularized EBs revealed that the endothelial structures formed functional vessels that facilitated diffusion. The diffusion coefficient D for doxorubicin was 296 x 10(-9) cm2 s(-1) in vascularized 8-day-old EBs, ie, about 10-fold larger than in avascular 3-day-old EBs (18 x 10(-9) cm2 s(-1)) and EBs treated with suramin (14 x 10(-9) cm2 s(-1)), tamoxifen (13.5 x 10(-9) cm2 s(-1)), and tetrahydrocortisol/heparin (18.5 x 10(-9) cm2 s(-1)). Consequently, avascular EBs treated with antiangiogenic agents developed central necrosis, which was absent in vascularized EBs. Our findings indicate that EBs are a suitable in vitro model system to study the effects of antiangiogenic agents in a three-dimensional tissue context. Furthermore, EBs provide a unique model to investigate the diffusion of anticancer agents in a tissue in both the avascular and vascularized states.
Salicylaldehyde Ester-Induced Chemoselective Peptide Ligations: Enabling Generation of Natural Peptidic Linkages at the Serine/Threonine Sites (2010)

A serine/threonine-based chemoselective ligation method is described. It uses an O-salicylaldehyde ester at the C-terminus, reacting with N-terminal serine or threonine to realize peptide ligations. The utility of the O-salicylaldehyde ester enables the rapid coupling reaction and the production of an N,O-benzylidene acetal intermediate, which is readily converted into natural peptidic linkages (Xaa-Ser/Thr) at the ligation site.
Respiratory syncytial virus infection and the immunocompromised host (1988)

From the Departments of Pediatrics and Microbiology, School of Medicine, State University of New York at Buffalo, and the Division of Infectious Diseases, Children's Hospital of Buffalo, Buffalo, NY.
Charge-insensitive qubit design derived from the Cooper pair box (2007)

Short dephasing times pose one of the main challenges in realizing a quantum computer. Different approaches have been devised to cure this problem for superconducting qubits, a prime example being the operation of such devices at optimal working points, so-called ``sweet spots.'' This latter approach led to significant improvement of ${T}_{2}$ times in Cooper pair box qubits [D. Vion et al., Science 296, 886 (2002)]. Here, we introduce a new type of superconducting qubit called the ``transmon.'' Unlike the charge qubit, the transmon is designed to operate in a regime of significantly increased ratio of Josephson energy and charging energy ${E}_{J}∕{E}_{C}$. The transmon benefits from the fact that its charge dispersion decreases exponentially with ${E}_{J}∕{E}_{C}$, while its loss in anharmonicity is described by a weak power law. As a result, we predict a drastic reduction in sensitivity to charge noise relative to the Cooper pair box and an increase in the qubit-photon coupling, while maintaining sufficient anharmonicity for selective qubit control. Our detailed analysis of the full system shows that this gain is not compromised by increased noise in other known channels.
Cylindrical Slot FSS Configuration for Beam-Switching Applications (2014)

A novel design for a beam-switching antenna using active cylindrical slot frequency selective surface (ACSFSS) is presented. The antenna system is composed of an omnidirectional monopole antenna and the ACSFSS, which employs a new technique of switching slot arrays. The ACSFSS is made up of 12 columns with 8 slots each, dividing the cylinder by 30°. To steer the beam of the antenna the diodes are set off and on, so that the radiation pattern of the antenna is determined by the number of off state columns. To estimate the general dimension of the cylindrical FSS, an equivalent metallic reflector is introduced and optimized, and then parametric studies for the unit cell dimensions are discussed. The fabricated prototype works within the WLAN band, centered around 2.45 GHz, and can agilely select either a narrow-beam or wide-beam operating mode. Simulation and measurements confirm the operation of the ACSFSS antenna, with good matching and gain observed. In particular, the narrow-beam mode -3 dB beamwidth is 47° which offers enhanced angular resolution compared with other reported beam-sweeping work.
Synthesis and maturation of recombinant human tumor necrosis factor in eukaryotic systems (1986)

The biosynthesis of human tumor necrosis factor (hTNF) was studied. The amino-terminal extension of the hTNF precursor (26 kDa polypeptide) was not cleaved off in a cell-free system supplemented with dog pancreas microsomes. Correct maturation of pre-hTNF was nevertheless not restricted to the macrophage system: in the medium of a TNF-producing, transformed CHO cell line, a (weak) approximately 20 kDa, an approximately 18.5 kDa (doublet) and a 17 kDa TNF polypeptide, the latter corresponding to mature hTNF, were revealed by specific immunoprecipitation. Similar results were obtained with Xenopus laevis oocytes, injected with hTNF mRNA, except that the 20 kDa band was lacking. The results are discussed in relation to the secretion mechanism of hTNF.
Split menus (1994)

When some items in a menu are selected more frequently than others, as is often the case, designers or individual users may be able to speed performance and improve preference ratings by placing several high-frequency items at the top of the menu. Design guidelines for split menus were developed and applied. Split menus were implemented and tested in two in situ usability studies and a controlled experiment. In the usability studies performance times were reduced by 17 to 58% depending on the site and menus. In the controlled experiment split menus were significantly faster than alphabetic menus and yielded significantly higher subjective preferences. A possible resolution to the continuing debate among cognitive theorists about predicting menu selection times is offered. We conjecture and offer evidence that, at least when selecting items from pull-down menus, a logarithmic model applies to familiar (high-frequency) items, and a linear model to unfamiliar (low-frequency) items.
Frequency stabilization of gas lasers (1965)

The state of the art in the field of frequency stabilization of gas lasers is surveyed. A brief discussion of the methods employed to determine the frequency stability of lasers is followed by a listing of the principle causes of frequency instability. The close relationship existing between the control system design and the laser environment is pointed out. Stabilization techniques based on the use of atomic resonance and on the use of interferometers are discussed in detail. Many of these techniques are capable of achieving long-term frequency stabilities of one part in 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">9</sup> or better, which appears to be entirely adequate for most systems applications.
Molecular characterization of the ferric-uptake regulator, Fur, from Staphylococcus aureus The GenBank accession numbers for the S. aureus fur gene and fhu operon reported in this paper are AF118839 and AF132117, respectively. (2000)

Iron is an essential nutrient for the survival and pathogenesis of bacteria, but relatively little is known regarding its transport and regulation in staphylococci. Based on the known sequences of ferric-uptake regulatory (fur) genes from several Gram-positive and Gram-negative bacteria, a fragment containing the fur homologue was cloned from a genomic library of Staphylococcus aureus RN450. Nucleotide sequence analysis of this fragment revealed the presence of a 447 bp ORF that encodes a putative 149 aa polypeptide with an apparent molecular mass of 17 kDa. A putative ferrichrome-uptake (fhu) operon, containing the conserved Fur-binding sequences (Fur box) in the promoter region, was also cloned from the same S. aureus library. To characterize the impact of Fur on the fhu operon, fur was cloned, overexpressed as a His-tagged protein and purified by Ni2+-affinity column chromatography. The recombinant protein was digested with enterokinase to remove the His tag. Electrophoretic mobility-shift assays indicated that Fur binds to the promoter region of the fhu operon in the presence of divalent cations. Fur also interacted with the promoter region of the recently reported sir operon that has been proposed to constitute a siderophore-transport system in S. aureus. The DNase I-protection assay revealed that Fur specifically binds to the Fur box located in the promoter region of the fhu operon. The primer-extension reaction indicated that the transcription-start site of the fhu operon was located inside the Fur box. S. aureus fur partially complemented a fur- mutation in Bacillus subtilis. The data suggest that Fur regulates iron-transport processes in S. aureus.
Advanced power management techniques in next-generation wireless networks [Topics in Wireless Communications] (2010)

Because mobile devices are equipped with a limited amount of battery power, it is essential to have efficient power management mechanisms in mobile broadband networks such as mobile WiMAX and 3GPP Long Term Evolution that enable always on connectivity. This article presents the state-of-the-art power management methods in next-generation wireless networks with a focus on IEEE 802.16m based next-generation WiMAX networks and 3GPP LTE. To minimize and optimize user equipment power consumption, and further to support various services and large amounts of data transmissions, advanced power conservation mechanisms are being developed in IEEE 802.16m and 3GPP. Two advanced power conservation mechanisms, sleep and idle modes, which are enhanced versions of the legacy IEEE 802.16 system's sleep and idle modes, were proposed and adopted in IEEE 802.16m. Similarly, 3GPP LTE adopts a discontinuous reception mechanism for power conservation in RRC_CONNECTED and RRC_IDLE states. Power management techniques in WiMAX and 3GPP LTE provide less control signaling and operational overhead while providing more efficient power saving, and use simpler operation procedures than the existing power management techniques.
Micro-NMR for Rapid Molecular Analysis of Human Tumor Samples (2011)

Although tumor cells obtained from human patients by image-guided intervention are a valuable source for diagnosing cancer, conventional means of analysis are limited. Here, we report the development of a quantitative micro-NMR (nuclear magnetic resonance) system for rapid, multiplexed analysis of human tumors. We implemented the technology in a clinical setting to analyze cells obtained by fine-needle aspirates from suspected lesions in 50 patients and validated the results in an independent cohort of another 20 patients. Single fine-needle aspirates yielded sufficient numbers of cells to enable quantification of multiple protein markers in all patients within 60 min. Moreover, using a four-protein signature, we report a 96% accuracy for establishing a cancer diagnosis, surpassing conventional clinical analyses by immunohistochemistry. Our results also show that protein expression patterns decay with time, underscoring the need for rapid sampling and diagnosis close to the patient bedside. We also observed a surprising degree of heterogeneity in protein expression both across the different patient samples and even within the same tumor, which has important implications for molecular diagnostics and therapeutic drug targeting. Our quantitative point-of-care micro-NMR technique shows potential for cancer diagnosis in the clinic.
Use of Kv1.3 Blockers for Inflammatory Skin Conditions (2010)

Recent results using animal models of inflammatory skin conditions have shown that blockers of the voltage-gated potassium channel, Kv1.3 hold great promise for clinical utility. Kv1.3 blockers act as immunosuppressants by modulating the various subsets of inflammatory T and B cells involved in autoimmune disorders. While peptidic inhibitors based on naturally occurring venoms demonstrate potent and selective Kv1.3 blockade, these require parenteral administration and may face potential immunogenicity problems. Small molecule blockers show considerable diversity, however selectivity over other Kv1-family channels has been difficult to achieve. More recent advances have added to the evidence that Kv1.3 channels are a suitable therapeutic target and that the development of novel and selective agents will herald new drugs for inflammatory skin disorders.
Multidimensional transcriptomics provides detailed information about immune cell distribution and identity in HER2+ breast tumors (2018)

Abstract The comprehensive analysis of tumor tissue heterogeneity is crucial for determining specific disease states and establishing suitable treatment regimes. Here, we analyze tumor tissue sections from ten patients diagnosed with HER2+ breast cancer. We obtain and analyze multidimensional, genome-wide transcriptomics data to resolve spatial immune cell distribution and identity within the tissue sections. Furthermore, we determine the extent of immune cell infiltration in different regions of the tumor tissue, including invasive cancer regions. We combine cross-sectioning and computational alignment to build three-dimensional images of the transcriptional landscape of the tumor and its microenvironment. The three-dimensional data clearly demonstrates the heterogeneous nature of tumor-immune interactions and reveal interpatient differences in immune cell infiltration patterns. Our study shows the potential for an improved stratification and description of the tumor-immune interplay, which is likely to be essential in treatment decisions.