gebhart/patrepeval huggingface dataset:
Derivatization of amino acids with N,N ‐dimethyl‐2,4‐dinitro‐5‐fluorobenzylamine for liquid chromatography/electrospray ionization mass spectrometry (2004)

Derivatization, separation and identification of amino acids with a novel compound, N,N-dimethyl-2,4-dinitro-5-fluorobenzylamine (DMDNFB), using high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) was demonstrated. Compared to derivatization with 2,4-dinitrofluorobenzene (DNFB), DMDNFB-derivatized amino acids and dipeptides exhibit much larger ion current signals in the commonly used ESI positive mode, which was attributed to the introduction of the N,N-dimethylaminomethyl protonatable site.
A review of the methods of processing EMG for use as a proportional control signal. (1976)

The use of EMG as a proportional control signal for prostheses is reviewed. The lack of success of existing proportional EMG-controlled prostheses is shown to be attributable in the greater part to the presence of low frequency "noise" in the processed EMG signal. If EMG is to yield useful proportional information, this noise must be eliminated, and to this end the methods of processing EMG are reviewed and the sources of noise examined. A number of methods for its removal are discussed.
Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor (2000)

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220(+)BP-1(+) pre-B cells.
Novel Triazole Ribonucleoside Down-Regulates Heat Shock Protein 27 and Induces Potent Anticancer Activity on Drug-Resistant Pancreatic Cancer (2009)

A series of novel 3-arylethynyltriazolyl ribonucleosides were synthesized and assessed for their anticancer activity on the drug-resistant pancreatic cancer cell line MiaPaCa-2. Among them, one compound exhibited potent apoptosis-inducing properties and anticancer activity against the pancreatic cancer model MiaPaCa-2 both in vitro and in vivo with no adverse effects. This compound did not inhibit DNA synthesis and therefore does not resemble the clinical drug gemcitabine. It did, however, significantly down-regulate the expression of heat shock protein 27 (Hsp27), a small molecular chaperone playing an important role in drug resistance and highly expressed in drug-resistant cancer forms, and thus represents the first small molecular anticancer lead with such a mode of action.
Collection of mRNA-like non-coding RNAs (1999)

In last few years much data has accumulated which shows that in different cells various RNA transcripts are synthesized. They lack protein coding capacity and do not produce mature protein. It seems that they work mainly or exclusively on the RNA level. Their function and mechanism of action is poorly understood. In this paper we have collected all known RNA transcript and prepared a database for further structural and functional studies. This is the first collection of the nucleotide sequences of RNAs of this kind. The data can be accessed via WWW at: http://www.man.poznan.pl/5SData/ncRNA/inde x.html
A VLSI implementation of MIMO detection for future wireless communications (2004)

This paper describes a VLSI implementation of V-BLAST detection for future multiple-input-multiple-output (MIMO) wireless communications. This design is implemented using a 0.35-/spl mu/m 5-layer metal 3.3 V CMOS technology. For a 4-transmit and 4-receive antennas system using QPSK modulation scheme, a detecting throughput of 128 Mb/s can be achieved. Furthermore, it is shown that the implementation complexity can be reduced further to meet the requirements for future high speed downlink packet access (HSDPA) systems with MIMO extensions in 3/sup rd/ generation (3G) mobile wireless systems.
A simplified but robust method for the isolation of avian and mammalian muscle satellite cells (2012)

Our simplified method presents a quick and simple alternative to isolating highly enriched muscle satellite cell cultures which can subsequently rapidly differentiate into well developed primary myotubes. The use of the same isolation protocol allows better inter-species comparisons of muscle satellite cells. Of all the farm animal species investigated, harvested chicken muscle cells showed the highest percentage of muscle satellite cells, and equine muscle cells presented the highest fusion index, an impressive ≈ 77%. Porcine cells displayed the lowest amount of satellite cells but still achieved a modest fusion rate of ≈ 41%.
The identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in Bordetella pertussis (1996)

Bordetella pertussis lipopolysaccharide (LPS) is biologically active, being both toxic and immunogenic. Using transposon mutagenesis we have identified a genetic locus required for the biosynthesis of LPS in B. pertussis, which has been cloned and sequenced. We have also identified equivalent loci in Bordetella bronchiseptica and Bordetella parapertussis and cloned part of it from B. parapertussis. The amino acid sequences derived from most of the genes present in the sequenced B. pertussis locus are similar to proteins required for the biosynthesis of LPS and other complex polysaccharides from a variety of bacteria. The genes are in a unique arrangement in the locus. Several of the genes identified are similar to genes previously shown to play specific roles in LPS O-antigen biosynthesis. In particular, the amino acid sequence derived from one of the genes is similar to the enzyme encoded by rfbP from Salmonella enterica, which catalyses the transfer of galactose to the undecaprenol phosphate antigen carrier lipid as the first step in building oligosaccharide O-antigen units, which are subsequently assembled to form polymerized O-antigen structures. Defined mutation of this gene in the B. pertussis chromosome results in the inability to express band A LPS, possibly suggesting that the trisaccharide comprising band A is a single O-antigen-like structure and that B. pertussis LPS is similar to semi-rough LPS seen in some mutants of enteric bacteria.
Phase retrieval of arbitrary complex-valued fields through aperture-plane modulation (2007)

We propose and experimentally demonstrate a simple and robust approach for retrieving arbitrary complex-valued fields from three or more diffraction intensity recordings. We need no a priori knowledge about the object field. The convergence rate is rapid. We obtained good results using experimental data with only 80 iterations (160 fast Fourier transforms). The method does not suffer any stagnation or ambiguity problem, and it also exhibits a high immunity to noise. The technique exhibits great potential in lensless phase-contrast imaging, wave-front sensing, and metrology for a wide spectral range.
The Car as a Peripheral, Adapting a Portable Computer to a Vehicle Intranet (1998)

This paper examines the feasibility of integrating a Handheld Personal Computer (HPC), cellular telephone and modem to integrate with a vehicle's audio, climate and system controls. A standard infrared serial data link is used to establish connectivity between the HPC and the vehicle. A cellular telephone provides voice connectivity outside the vehicle and a Cellular Digital Packet Data (CDPD) modem is used for data connectivity. The paper also describes how the system is built into a Dodge ESX-2 hybrid powered concept vehicle.
Platelet aggregation in partially obstructed vessels and its elimination with aspirin. (1976)

In 35 open chest anesthetized dogs coronary and aortic blood flow were measured with electromagnetic flowmeters while aortic and distal coronary blood pressure and an epicardial ECG were recorded. A fixed amount of stenosis (60-80%) was produced in the coronary artery by an externally applied plastic cylinder. In 24 of the 35 dogs the coronary blood flow showed cyclical reductions to near zero, with a sudden spontaneous return to near control levels. During reduced flow the epicardial ECG showed ST-segment depression suggestive of ischemia, and ventricular premature beats were often noted. Six animals died acutely during episodes of reduced flow. After 35 mg/kg of aspirin were given intravenously the cyclical reductions in coronary blood flow were abolished and the in vitro platelet aggregations were reduced from a control of 62.1 +/- 15 units (Born technique) to an average of 23.7 +/- 12 units. Histologic sections of the narrowed coronary artery obtained when coronary flow was reduced show an amorphous mass in the lumen which was thought to be a platelet aggregate. Perhaps a similar process of platelet aggregation occurs in the stenosed coronary arteries in man, producing acute coronary obstruction, ischemia, and sudden death.
Virus-Associated Receptors for Polymerized Human Serum Albumin (RpHSA) in Patients with Chronic Active Hepatitis B Treated with Recombinant Leukocyte A Interferon (1987)

Hepatitis B surface antigen (HBsAg)-associated receptors for polymerized human serum albumin (RpHSA) are assumed to mediate viral attachment to hepatocytes in hepatitis B virus (HBV) infection. RpHSA was found to be coded by the pre-S region of HBV genome. Recently, the antiviral effect of recombinant leukocyte alpha-interferon was shown in patients with hepatitis B. Our study evaluated the detection and the clinical implications of RpHSA in patients with chronic active hepatitis B under treatment with recombinant alpha-interferon. Two out of nine patients eliminated all HBV markers including RpHSA. Four out of nine patients became negative for markers of viral replication but remained positive for HBsAg and in part for RpHSA. In three out of nine patients HBV markers including RpHSA remained unchanged. In summary, the titer for RpHSA is a reliable indirect marker for infectivity and of prognostic value in patients with chronic active hepatitis B during interferon treatment. Future studies should evaluate a putative immune response to RpHSA-containing viral surface proteins, which could be relevant for viral clearance in HBV infection.
Tumour lymphangiogenesis is a possible predictor of sentinel lymph node status in cutaneous melanoma: a case–control study (2006)

This study provides evidence that the presence of high peritumorous and intratumorous lymphatic microvessel density is associated with SLN metastasis and shorter survival. The intratumorous lymphatic vessel area is the most significant factor predicting SLN metastasis. The tumour associated lymphatic network constitutes a potential criterion in the selection of high risk patients for complementary treatment and a new target for antimelanoma therapeutic strategies.
연구논문 : 유전독성 대체시험법(in vitro소핵시험)의 국내검증시험연구 (2011)

This validation study was performed to introduce and set up the in vitro micronucleus test, as an alternative to the existing chromosome aberration test, in the test facilities in Korea, and to establish the competence of each facility to perform the in vitro micronucleus test independently. The 10 coded substances, including 6 positive and 4 negative in genotoxicity assay, were tested. Six facilities were divided into two subgroups, A and B. Three coded positive compounds and two negative compounds were sent to each test facility. The compounds were tested according to the facility`s GLP system. In group A, positive results were obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. In group B, positive results were also obtained from testing of the three positive compounds, and negative results were obtained from testing of the two negative compounds in all three facilities. For the Urethane, an in vitro equivocal compound tested by group B, negative results were obtained. These results suggested that the compound may be effectively activated only in in vivo environment. The results show that the six test facilities are capable of performing the in vitro micronucleus test independently and producing consistent results.
Porphyria Cutanea Tarda in a Patient with End-Stage Renal Disease: A Case of Successful Treatment with Deferoxamine and Ferric Carboxymaltose (2017)

<i>Porphyria cutanea tarda</i> (PCT) is a rare disease, with a strong association with hepatitis C virus. PCT is particularly problematic in end-stage renal disease patients as they have no renal excretion of porphyrins and these are poorly dialyzed. Also, conventional treatment of PCT is compromised in these patients as hydroxychloroquine is contraindicated, phlebotomies with the stipulated frequency are poorly tolerated in already anaemia-prone patients, and iron-chelating agents are less efficient in removing iron and contribute to worsening anaemia. The authors report a patient on haemodialysis, with hepatitis C infection, that is diagnosed with PCT. Despite the good clinical results with deferoxamine, she became dependent on blood transfusions because of her ferropenic state. Every time oxide iron was started, the patient developed clinical features of the disease, resolving after the suspension of the drug. A decision was made to start the patient on ferric carboxymaltose, which was well tolerated without disease symptoms and need of further blood transfusions. This case suggests that deferoxamine is efficient in treatment of porphyria cutanea tarda. Also, ferric carboxymaltose may be a valuable option for refractory anaemia in patients with this disease and end-stage renal disease, as it seems to provide iron without clinical relapse of the disease.
A Proteomic Analysis of Lysosomal Integral Membrane Proteins Reveals the Diverse Composition of the Organelle (2004)

Lysosomes are endocytic subcellular compartments that contribute to the degradation and recycling of cellular material. Using highly purified rat liver tritosomes (Triton WR1339-filled lysosomes) and an ion exchange chromatography/LC-tandem MS-based protein/peptide separation and identification procedure, we characterized the major integral membrane protein complement of this organelle. While many of the 215 proteins we identified have been previously associated with lysosomes and endosomes, others have been associated with the endoplasmic reticulum, Golgi, cytosol, plasma membrane, and lipid rafts. At least 20 proteins were identified as unknown cDNAs that have no orthologues of known function, and 35 proteins were identified that function in protein and vesicle trafficking. This latter group includes multiple Rab and SNARE proteins as well as ubiquitin. Defining the roles of these proteins in the lysosomal membrane will assist in elucidating novel lysosomal functions involved in cellular homeostasis and pathways that are affected in various disease processes.
The Rechargeable LiAl / Li3.6Ge0.6 V 0.4 O 4 / TiS2 High Temperature Solid‐State Cell (1994)

An all solid‐state high temperature cell is described using a solid solution of lithium germanium oxide and lithium vanadium oxide with a general formula, , as the lithium ion conducting solid electrolyte. The cyclic voltammetric experiments with the solid electrolyte exhibited the reversible deposition of lithium metal at platinum and gold working electrodes at potentials below ca. 0.5 V vs. the lithium‐aluminum reference and showed the solid electrolyte to be electrochemically stable toward any oxidation up to a potential of ca. 4.5 V vs. a lithium‐aluminum reference electrode. The cells employed a lithium‐aluminum alloy as the anode and a chemical vapor deposited thin film of titanium disulfide as the cathode, and exhibited an open‐circuit potential of ca. 2.1 V at 300°C. The cells exhibited excellent high rate capabilities and were discharged at current densities up to 100 mA/cm2.
How do humans sketch objects? (2012)

Humans have used sketching to depict our visual world since prehistoric times. Even today, sketching is possibly the only rendering technique readily available to all humans. This paper is the first large scale exploration of human sketches. We analyze the distribution of non-expert sketches of everyday objects such as 'teapot' or 'car'. We ask humans to sketch objects of a given category and gather 20,000 unique sketches evenly distributed over 250 object categories. With this dataset we perform a perceptual study and find that humans can correctly identify the object category of a sketch 73% of the time. We compare human performance against computational recognition methods. We develop a bag-of-features sketch representation and use multi-class support vector machines, trained on our sketch dataset, to classify sketches. The resulting recognition method is able to identify unknown sketches with 56% accuracy (chance is 0.4%). Based on the computational model, we demonstrate an interactive sketch recognition system. We release the complete crowd-sourced dataset of sketches to the community.
Synthesis of the polyether antibiotic monensin. 2. Preparation of intermediates (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of the polyether antibiotic monensin. 2. Preparation of intermediatesDavid B. Collum, John H. McDonald III, and W. Clark StillCite this: J. Am. Chem. Soc. 1980, 102, 6, 2118–2120Publication Date (Print):March 1, 1980Publication History Published online1 May 2002Published inissue 1 March 1980https://pubs.acs.org/doi/10.1021/ja00526a074https://doi.org/10.1021/ja00526a074research-articleACS PublicationsRequest reuse permissionsArticle Views2012Altmetric-Citations128LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Spherical harmonic lighting : the gritty details (2003)

Spherical Harmonic lighting (SH lighting) is a technique for calculating the lighting on 3D models from area light sources that allows us to capture, relight and display global illumination style images in real time. It was introduced in a paper at Siggraph 2002 by Sloan, Kautz and Snyder as a technique for ultra realistic lighting of models. Looking a little closer at it’s derivation we can show that it is in fact a toolbox of interrelated techniques that the games community can use to good effect.
Osmium‐ and Ruthenium‐Based Phosphorescent Materials: Design, Photophysics, and Utilization in OLED Fabrication (2006)

Abstract Osmium(II) complexes possessing β‐diketonate, quinolinate, diimine, and C‐linked pyridyl azolate chelates reveal interesting structural and photophysical properties. Spectroscopic and dynamic measurements, in combination with theoretical analyses, have provided an important understanding of the electronically excited state properties of these complexes, such as the energy gap and nature of the lower lying states, rate for intersystem crossing, and the efficiency of corresponding radiative decay and nonradiative deactivation processes. This review also reports on the synthetic processes that lead to the neutral Os II and Ru II complexes that possess two trans ‐substituted phosphane donor ligands together with two anti ‐parallel, aligned azolate chromophores. Considerable efforts have been made to focus on utilizing these emitting materials as phosphorescent dopants for practical PLED and OLED fabrication. Consequently, the interplay between these emitting materials and device configurations is discussed. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2006)
Plasma and skin carriers for natural and synthetic retinoids (1987)

• Human skin contains several proteins that could bind the hydrophobic retinoids. One is retinol-binding protein (RBP), the plasma carrier of natural vitamin A, retinol, albumin, and two specific cellular-binding proteins, one for retinol (CRBP) and one for retinoic acid (CRABP). Adequate techniques for the study of these proteins in human skin have been developed; this was considered a necessary step in the understanding of how natural and synthetic retinoids work in the skin. A polyacrylamide gel electrophoresis technique followed by protein immunoblot analysis with an antiserum specific against RBP was developed. This technique allowed study of the binding of several natural and synthetic retinoids on the plasma carrier of natural vitamin A for the first time; it was found that only natural retinoids, including retinoic acid, bind to RBP in vitro; retinoic acid binding was found to induce major conformational changes in the protein. Isotretinoin did not bind to RBP. The technique was then used for the study of RBP in human epidermal and dermal extracts; it showed that RBP degradation with loss of binding properties for retinol occurred within the epidermis; this suggests that retinol supply is decreased within the epidermis, a fact that could be linked to cornification. A polyacrylamide gel electrophoresis binding assay was developed for studying CRBPs and CRABPs in human skin cytosolic extracts. This assay is more specific and more convenient than previous ones for studying tissue extracts. The CRABP was (1) in much higher levels in normal epidermis than in normal dermis, (2) dramatically elevated in psoriatic plaques and in some retinoid-responsive dermatoses, and (3) up modulated by both topical and systemic administration of natural and synthetic retinoids. Such alterations were not observed for CRBP. From these and other observations reviewed herein, it seems that CRABP should be one of the first candidates to be carefully analyzed in the search of the cellular receptor for the mediation of the synthetic retinoids in pharmacologic effects in human skin. (<i>Arch Dermatol</i>1987;123:1690a-1692a)
Gene-Based Immunotherapy for Human Immunodeficiency Virus Infection and Acquired Immunodeficiency Syndrome (2006)

More than 40 million people are infected with human immunodeficiency virus (HIV), and a successful vaccine is at least a decade away. Although highly active antiretroviral therapy prolongs life, the maintenance of viral latency requires life-long treatment and results in cumulative toxicities and viral escape mutants. Gene therapy offers the promise to cure or prevent progressive HIV infection by interfering with HIV replication and CD4+ cell decline long term in the absence of chronic chemotherapy, and approximately 2 million HIV-infected individuals live in settings where there is sufficient infrastructure to support its application with current technology. Although the development of HIV/AIDS gene therapy has been slow, progress in a number of areas is evident, so that studies to date have significantly advanced the field of gene-based immunotherapy. Advances have helped to define a series of ongoing and planned trials that may shed light on potential mechanisms for the successful clinical gene therapy of HIV.