gebhart/patrepeval huggingface dataset:
Mobility management in next generation networks (2009)

La definizione che l’ITU-T fornisce delle “Next Generation Network” (NGN) include la possibilità di poter utilizzare differenti tecnologie di accessi a banda larga e di supportare la mobilità. Inoltre le reti NGN hanno la necessità di integrare le diverse tecnologie di accesso alla rete IP in maniera del tutto trasparente. In questa tesi dapprima si definiscono i requisiti che una soluzione per la gestione della mobilità nei sistemi di comunicazione real-time dovrebbe avere. Successivamente si analizzano le soluzioni per la gestione della mobilità presenti in letteratura. A partire da quest’analisi nasce MMUSE (Mobility Management Using Extension) una soluzione per la gestione della mobilità a livello applicativo basata sul protocollo SIP in grado di soddisfare la maggior parte dei requisiti richiesti ad un sistema di gestione della mobilità fra reti eterogenee. Tale soluzione è stata inoltre implementata, per testare sul campo le sue capacità e la sua interoperabilità con diversi terminali e Server SIP. Inoltre, al fine di valutarne le prestazioni è stata effettuata una campagna di misure nel mondo reale utilizzando alcune reti di accesso commerciali (sia 3G che WiFi). Infine, si propone una metodologia per la valutazione delle prestazioni delle differenti soluzioni di gestione della mobilità precedentemente illustrate. Tale metodologia si compone di un semplice modello analitico applicabile sia ad un caso ideale (senza perdite) che un caso reale con un predefinito tasso di perdita dei pacchetti. Il modello è stato applicato a tre soluzioni MMUSE, MIPv4 e SIP re-INVITE.
Loss of heterozygosity of chromosome 17 in human borderline and invasive epithelial ovarian tumors. (1996)

Polymerase chain reaction (PCR) analysis of microsatellite polymorphisms corresponding to four loci which map to chromosome 17p and 11 loci which map to chromosome 17q was performed to screen for loss of heterozygosity (LOH) in paired normal and tumor tissues from 27 cases of borderline epithelial ovarian tumors (BEOT) and 32 cases of invasive epithelial ovarian cancers (IOC). LOH was observed in six of 27 (22%) of the borderline tumors and in 29 of 32 (90%) of the invasive ovarian cancers (P<0.001). At all 15 loci studied, a lower percentage of allelic loss was detected in borderline tumors (0-14%) vs invasive cancer (8-93%). At eight loci this difference was statistically significant. For IOC, one common loss region was identified on chromosome 17p and four distinct common loss regions were on chromosome 17q, which supports the notion that multiple tumor suppressors may reside on chromosome 17 in IOC. These data suggest that LOH on chromosome 17 is an infrequent event in BEOT compared with IOC and therefore may not be important in the distinct pathogenesis of BEOT.
Chlorin-based symmetrical and unsymmetrical dimers with amide linkages: effect of the substituents on photodynamic and photophysical properties (2000)

In this study, we report syntheses, in vivo biological activity, and photophysical properties of a series of chlorin-based symmetrical and unsymmetrical dimers with amide linkages. All compounds exhibited strong absorption maxima at wavelengths ranging between λmax 660 and 702 nm. Compared with the formylpyropheophorbide a dimer 7 and purpurin 18 dimer 9 containing electron-withdrawing substituents at peripheral positions, pyropheophorbide a dimer 6, 3-devinyl-3-(1-hexyloxyethyl)pyropheophorbide a dimer 8, and unsymmetrical dimer 12 in which the chlorin e6 and 3-devinyl-3-(1-hexyloxyethyl)pyropheophorbide a moieties are linked with amide bonds, produced high fluorescence yields. For all photosensitizers, energy transfer from the sensitizer triplet to the ground state of oxygen is irreversible with rate constants kTΣ ≈ 2 × 109 M−1 s−1, a value in the diffusion-limited rate range. This energy transfer resulted in relatively high singlet oxygen quantum yields (ΦΔ ≈ 0.50 for compounds 12 and 8; and ΦΔ ≈ 0.30 for compounds 6 and 7). Among these dimers, compound 9 with a six-membered anhydride ring system produced the lowest singlet oxygen quantum yield (ΦΔ 0.06). The in vivo PDT efficacy of these compounds was evaluated in DBA/2 mice bearing SMT/F tumors. Among all the dimers, the unsymmetrical dimer 12 was found to be most effective, but it was significantly less active than the related monomer 3-devinyl-3-(1-hexyloxyethyl)pyropheophorbide a2.
Structure and Enzymatic Properties of Genetically Truncated Forms of the Water-Insoluble Glucan-Synthesizing Glucosyltransferase from Streptococcus sobrinus (1999)

Glucosyltransferase-I (GTF-I: 175 kDa) of a cariogenic bacterium, Streptococcus sobrinus 6715, mediates the conversion of water-soluble dextran (alpha-1,6-glucan) into a water-insoluble form by making numerous alpha-1,3-glucan branches along the dextran chains with sucrose as the glucosyl donor. The structures and catalytic properties were compared for two GTF-I fragments, GTF-I' (138 kDa) and GS (110 kDa). Both lack the N-terminal 84 residues of GTF-I. While GTF-I' still contains four of the six C-terminal repeats characteristic of streptococcal glucosyltransferases, GS lacks all of them. Electron microscopy of negatively stained samples indicated a double-domain structure for GTF-I', consisting of a spherical head with a smaller spherical tail, which was occasionally seen as a long extension. GS was seen just as the head portion of GTF-I'. In the absence of dextran, both fragments simply hydrolyzed sucrose with similar K(m) and k(cat) values at low concentrations (<5 mM). At higher sucrose concentrations (>10 mM), however, GTF-I' exhibited glucosyl transfer activity to form insoluble alpha-1, 3-glucans. So did GS, but less efficiently. Dextran increased the rate and efficiency of the glucosyl transfer by GTF-I'. On removal of the C-terminal repeats of GTF-I' by mild trypsin treatment, this dextran-stimulated transfer was completely lost and the dextran-independent transfer became less efficient. These results indicate that the N-terminal two-thirds of the GTF-I sequence are organized as a structurally and functionally independent domain to catalyze not only sucrose hydrolysis but also glucosyl transfer to form alpha-1,3-glucan chains, although not efficiently; the C-terminal repeat increases the efficiency of the intrinsic glucosyl transfer by the N-terminal domain as well as rendering the whole molecule primer-dependent for far more efficient insoluble glucan synthesis.
Rituximab for the treatment of acquired antibodies to factor VIII (2007)

All in all, both treatment schemes are effective therapies in patients with spontaneous antibodies to factor VIII. Our data analysis is only descriptive and no conclusions can be drawn as to the relative efficacy of the two regimens. However, these data may serve as a useful basis for planning randomized studies to definitively resolve these issues.
Artifact reduction in photoplethysmography (1998)

Motion artifact reduction in photoplethysmography, and therefore by implication in pulse oximetry, is achieved with a novel nonlinear methodology. The physical origins of the photoplethysmographic signals are explored in relation to a nonlinear measure of the observed intensity fluctuations. It is demonstrated that the nonlinearity renormalizes the received pulsations with optical information in a manner that aids physical interpretation. A heuristic physical model for the motion artifact is introduced and experimentally justified, with an inversion for artifact reduction being simplified by the nonlinearity. A practical implementation technique is discussed with emphasis placed on the resultant rescaling of the static and the dynamic portions of the signals. It is noted that this implementation also has the desirable effect of reducing any residual ambient artifact. The scope and power of this methodology is investigated with the presentation of results from a practical electronic system.
Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells (2007)

We have studied the plasma membrane protein phenotype of human culture-amplified and native bone marrow mesenchymal stem cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in colony-forming units-fibroblasts was low for CD49b, CD90, and CD105, but high for CD73, CD130, CD146, CD200, and integrin alphaV/beta5. In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies.
A new method for describing predator‐prey relations in discontinous environments (1984)

Summary I propose a new method for anlysing predatorprey interactive systems in discontinuous environments. The basic index used here is a generalized version of Lloyd 's (1967) “interspecies mean crowding”, which is defined as the number of individuals of one species existing in a given patch per that of the other species in either the same or different patches at either the same or different times. Four indices are derived from different combinations of the numbers of the prey and the predator in habitat patches. Then, the correlation coefficients between the numbers of individuals in patches in both different locations and times are derived by modifying the above new indices. Using this technique, dynamical changes of the joint distributions of the numbers of predators and prey which reflect variation in local conditions, can readily be described. As an example, this method was applied to an analysis of the outcomes of a multi‐patch version of the Lotka‐Volterra model of predator‐prey interactions.
Restoration in vivo of erythrocyte adenosine triphosphate, 2,3-diphosphoglycerate, potassium ion, and sodium ion concentrations following the transfusion of acid-citrate-dextrose-stored human red blood cells. (1969)

Abstract With the use of a manual differential agglutination procedure to recover liquid-preserved group O red cells after transfusion to group A 1 recipients, changes in intracellular levels of 2,3-DPG, ATP, sodium ion, and potassium ion were studied. A significant increase in cellular 2,3-DPG occurred immediately at the completion of the 234 hour transfusions. Within 3 hours following transfusion the level of 2,3-DPG was at least 1.27 μmoles per 10 10 RBC, 1.12 μmoles per milliliter of RBC, 3.48 μmoles per gram of Hb, or 0.233 moles per mole of Hb. The level of 2,3-DPG was above 50 per cent of the final level within 24 hours after transfusion, and it then continued to increase gradually up to 11 days. The ATP level of the transfused red cells increased rapidly, a change that was associated with a rapid decrease of the donor cell sodium ion content during the 24 hour posttransfusion period. Donor cellular potassium ion concentration increased slowly; the rate of rise appeared to be related to the increasing level of intracellular 2,3-DPG. Other investigators have established a relation between the organic phosphates, ATP and 2,3-DPG, and oxyhemoglobin dissociation characteristics of preserved red cells. The methods used for red cell preservation ideally should maintain the levels of organic phosphates, so that the cells will have normal affinity for oxygen at the time of transfusion.
Strong Means Slow: Dynamic Contributions to the Bulk Mechanical Properties of Supramolecular Networks (2005)

Hold on tight: The dynamics, rather than thermodynamics, of reversible cross-links are the primary determinants of bulk viscosities in a family of supramolecular networks. This result comes from a study in which a series of structurally similar pincer complexes (see picture, OTf=CF3SO3−) that have different ligand-exchange dynamics is used to cross-link poly(4-vinylpyridine). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z500026_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Use of a colorimetric system for yeast susceptibility testing (1995)

We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified with the Alamar reagent and a microdilution method modified with the Alamar reagent. The MICs of fluconazole and itraconazole for 97 isolates of C. albicans and 3 control isolates were tested. For fluconazole, the Alamar-modified broth macrodilution method yielded 94% (91 of 97) concordance within 2 dilutions compared with the NCCLS method, while the microdilution method yielded 95% (92 of 97) concordance. With Alamar-modified methods for itraconazole, broth macrodilution yielded 97% (94 of 97) concordance within 2 dilutions. MICs obtained by the microdilution method, although tightly nested, were shifted to a higher value when compared with those obtained by the NCCLS method; there was only 77% (75 of 97) concordance within 2 dilutions but 97% concordance (94 of 97) within 3 dilutions. Tests by all methods with quality control strains showed excellent reproducibilities. For fluconazole, the methods modified with the Alamar reagent yielded clear endpoints and excellent correlation for the broth macrodilution and microdilution methods. For itraconazole, the methods modified with the Alamar reagent yielded clear endpoints and were reproducible, but higher MICs were obtained by the microdilution methods compared with those obtained by the NCCLS methods.
Clinical Uses of In-Shoe Pressure Analysis in Podiatric Sports Medicine (2007)

Athletic injuries of the foot and lower extremity are commonly treated with custom foot orthoses. These devices usually provide immediate relief of the athlete's pain and dysfunction. Occasionally, however, they do not help, or even increase the patient's discomfort. We discuss a method of using in-shoe pressure-measurement systems to analyze the athletic patient's foot and lower-extremity function before and after treatment with custom foot orthoses, with a focus on sagittal plane biomechanics. Case histories are presented of athletes whose gait pathologies were identified and treated successfully using an in-shoe pressure-measurement system.
Proton pump inhibitors and gastric acid secretion (2001)

Peer Reviewed
Fabry–Perot metrology for displacements up to 50 mm (2005)

A system designed to apply Fabry-Perot interferometry to the measurement of displacements is described. Two adjacent modes of a Fabry-Perot cavity are probed, and both the absolute optical frequencies and their difference are used to determine displacements via changes in cavity length. Light is coupled to the cavity via an optical fiber, making the system ideal for remote sensing applications. Continuous interrogation is not necessary, as the cavity length is encoded in the free spectral range. The absolute uncertainty is determined to be below 10 pm, which for the largest displacement measured corresponds to a relative uncertainty of 4 x 10(-10). To my knowledge this is the smallest relative uncertainty in a displacement measurement ever demonstrated.
Simple and Sensitive Detection of Mutations in the Ras Proto-Oncogenes Using PNA-Mediated PCR Clamping (1996)

Point mutations in the ras proto-oncogenes are amongst the most frequent changes found in human malignancies (1) and may have prognostic importance. A variety of methods have been published for the detection of ras mutations (2-4). However, the most frequently used assays are limited either by an unsatisfactory sensitivity (2,3), the spectrum of detectable mutations (4) or require radioactive labeling (2,3). Here, we present a novel approach for the detection of ras point mutations based on the recently described method of peptide nucleic acid (PNA) mediated PCR clamping (5). PNAs are DNA-mimics where the phosphoribose backbone is replaced by a peptide-like repeat of (2-aminoethyl)-glycine units. Due to this chemical difference PNAs differ from DNA molecules in several aspects: (i) PNA/DNA-hybrids have a higher thermal stability compared with the corresponding DNA/DNA hybrids (1_C/base for mixed sequences); (ii) PNA/DNA hybrids are more destabilized by single base pair mismatches than the corresponding DNA/DNA hybrids (5); and (iii) PNAs could not serve as primer molecules in PCR.
Purification and characterization of recombinant equine infectious anemia virus reverse transcriptase (1991)

A 1.67-kb segment of the equine infectious anemia virus pol gene, encoding a 66-kDa reverse transcriptase (RT), was cloned and expressed in Escherichia coli. Recombinant RT, purified by a combination of metal chelate affinity chromatography and ion-exchange chromatography, displays both RNA-dependent DNA polymerase and RNase H activity. The affinity of purified RT for its replication primer, tRNA(3Lys) was equivalent to that observed for human immunodeficiency virus RT. Our data suggest that an additional domain between RT-RNase H and integrase on the equine infectious anemia virus pol open reading frame is not an integral component of the RT polypeptide.
Neuropsychological Outcomes of Army Personnel Following Deployment to the Iraq War (2006)

Deployment to Iraq is associated with increased risk of neuropsychological compromise. Findings point to the need to investigate further the impact of deployment on neural functioning. Public health implications include consideration of neuropsychological compromise in health prevention and postdeployment clinical and occupational management.
Human BAC Ends (2000)

The Human BAC Ends database includes all non-redundant human BAC end sequences (BESs) generated by The Institute for Genomic Research (TIGR), the University of Washington (UW) and California Institute of Technology (CalTech). It incorporates the available BAC mapping data from different genome centers and the annotation results of each end sequence for the contents of repeats, ESTs and STS markers. For each BAC end the database integrates the sequence, the phred quality scores, the map and the annotation, and provides links to sites of the library information, the reports of GenBank, dbGSS and GDB, and other relevant data. The database is freely accessible via the web and supports sequence or clone searches and anonymous FTP. The relevant sites and resources are described at http://www.tigr.org/ tdb/humgen/bac_end_search/bac_end_intro.html
Immunogenicity of Low-Dose Prime-Boost Vaccination of mRNA Vaccine CV07050101 in Non-Human Primates (2021)

Many different vaccine candidates against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, are currently approved and under development. Vaccine platforms vary from mRNA vaccines to viral-vectored vaccines, and several candidates have been shown to produce humoral and cellular responses in small animal models, non-human primates, and human volunteers. In this study, six non-human primates received a prime-boost intramuscular vaccination with 4 µg of mRNA vaccine candidate CV07050101, which encodes a pre-fusion stabilized spike (S) protein of SARS-CoV-2. Boost vaccination was performed 28 days post prime vaccination. As a control, six animals were similarly injected with PBS. Humoral and cellular immune responses were investigated at time of vaccination, and two weeks afterwards. No antibodies could be detected at two and four weeks after prime vaccination. Two weeks after boost vaccination, binding but no neutralizing antibodies were detected in four out of six non-human primates. SARS-CoV-2 S protein-specific T cell responses were detected in these four animals. In conclusion, prime-boost vaccination with 4 µg of vaccine candidate CV07050101 resulted in limited immune responses in four out of six non-human primates.
Phage presentation (1996)

There has recently been great interest in the use of the filamentous bacteriophage fd as a vehicle for the display of peptides and proteins. Phage libraries displaying random peptides up to 38 amino acids in length can be used (i) to select for ligands able to bind specific target molecules; (ii) to mimic non-proteinaceous ligands; and (iii) as a tool to map epitopes recognized by antibodies. The display of proteins or their functional domains provides a system for the analysis of structure-function relationships, and the potential to generate proteins with altered binding characteristics or novel catalytic properties. The display of short immunogenic determinants on fusion phage may provide a basis for the development of novel peptide vaccines, whilst the expression of libraries of antibody fragments may provide a method to by-pass hybridoma technology in the generation of monoclonal antibodies.
High-molecular-weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells (1983)

The carriers of the carbohydrate differentiation antigens I, i and SSEA-1 were investigated in embryonal carcinoma cell lines of mouse and differentiated cell lines derived from them. Glycoproteins were studied by immunostaining ('Western blotting') of total cell lysates and immunoprecipitation from lysates of galactose oxidase/NaB3H4-labelled cells; glycolipids were investigated by immunostaining of thin layer chromatograms. The antigenic activities detected by immunofluorescence of cell smears were reflected in the antigenicities of high-molecular-weight glycoproteins. These were polydisperse and markedly susceptible to digestion with endo-beta-galactosidase. Only the I antigen was detected on minor glycolipids. These observations indicate that glycoproteins rather than glycolipids are the major carriers of carbohydrate differentiation antigens I, i and SSEA-1 in the teratocarcinoma cell lines.
Driver Gaze Region Estimation Without Using Eye Movement (2015)

Automated estimation of the allocation of a driver's visual attention may be a critical component of future Advanced Driver Assistance Systems. In theory, vision-based tracking of the eye can provide a good estimate of gaze location. In practice, eye tracking from video is challenging because of sunglasses, eyeglass reflections, lighting conditions, occlusions, motion blur, and other factors. Estimation of head pose, on the other hand, is robust to many of these effects, but cannot provide as fine-grained of a resolution in localizing the gaze. However, for the purpose of keeping the driver safe, it is sufficient to partition gaze into regions. In this effort, we propose a system that extracts facial features and classifies their spatial configuration into six regions in real-time. Our proposed method achieves an average accuracy of 91.4% at an average decision rate of 11 Hz on a dataset of 50 drivers from an on-road study.
Regiodependent Luminescence Quenching of BiotinylatedN-Sulfonyl-acridinium-9-carboxamides by Avidin (2003)

[reaction: see text] Biotin was conjugated to chemiluminescent N-sulfonylacridinium-9-carboxamides at the N-10 or 9-position carboxamide. Upon binding to avidin, the light output of the N-10 derivative (8) was quenched up to 92% upon triggering with basic peroxide, while the 9-position carboxamide conjugate (9) was quenched only 33%. The utility of this effect was demonstrated in a model homogeneous chemiluminescence assay.