gebhart/patrepeval huggingface dataset:
Significance of quiescent smooth muscle migration in the injured rat carotid artery. (1985)

Cellular accumulation in the intima of injured artery has generally been attributed to smooth muscle cell proliferation. Since smooth muscle cells in normal artery are found mainly in the media, migration of smooth muscle cells into the intima has been considered a necessary prerequisite for subsequent myointimal thickening. The nondividing medial cells would appear to have no role in the reparative process. We have investigated in the rat ballooned carotid the possibility that nondividing cells might also contribute to injury-induced intimal thickening. All proliferating smooth muscle cells were labeled by 3H-thymidine given by continuous intraperitoneal infusion. The amounts of 3H-thymidine used were not toxic and did not inhibit smooth muscle cell proliferation. Autoradiograms performed on histological cross-sections showed a progressive decrease in the fraction of unlabeled cells at 3, 7, and 14 days after carotid injury. However, the actual number of nondividing cells remained constant. The calculated growth fraction for the 14-day period was 40%. A substantial number of unlabeled cells was observed in the intima. These data have led us to conclude that only a small fraction of smooth muscle cells in an artery proliferate in response to the injury stimulus, and do so shortly after injury, or not at all. Furthermore, nondividing, as well as proliferating smooth muscle cells, can migrate and contribute, in a substantial way, to the increase in intimal smooth muscle cell number.
Intraspinal stimulation for bladder voiding in cats before and after chronic spinal cord injury (2007)

The long-term objective of this study is to develop neural prostheses for people with spinal cord injuries who are unable to voluntarily control their bladder. This feasibility study was performed in 22 adult cats. We implanted an array of microelectrodes into locations in the sacral spinal cord that are involved in the control of micturition reflexes. The effect of microelectrode stimulation was studied under light Propofol anesthesia at monthly intervals for up to 14 months. We found that electrical stimulation in the sacral parasympathetic nucleus at S(2) level or in adjacent ventrolateral white matter produced bladder contractions insufficient for inducing voiding, while stimulation at or immediately dorsal to the dorsal gray commissure at S(1) level produced strong (at least 20 mmHg) bladder contractions as well as strong (at least 40 mm Hg) external urethral sphincter relaxation, resulting in bladder voiding in 14 animals. In a subset of three animals, spinal cord transection was performed. For several months after the transection, intraspinal stimulation continued to be similarly or even more effective in inducing the bladder voiding as before the transection. We speculate that in the absence of the supraspinal connections, the plasticity in the local spinal circuitry played a role in the improved responsiveness to intraspinal stimulation.
Preliminary characterization of phosphotyrosine phosphatase activities in human peripheral blood lymphocytes: Identification of CD45 as a phosphotyrosine phosphatase (1990)

A preliminary characterization of the protein phosphotyrosine phosphatase (PTPase) activity in human peripheral blood lymphocytes (PBL) has been made using two tyrosine-containing peptides and the epidermal growth factor receptor from A-431 cells as substrates. High PTPase activity with a pH optimum near 7.4 was observed in both the membrane and the cytosolic fractions. At least three distinct fractions with PTPase activity were separated on DEAE cellulose columns, indicating that the enzyme is heterogeneous. Vanadate, molybdate, and salts of zinc, copper, and mercury were all effective enzyme inhibitors, although the inhibition was generally incomplete and showed some variation with the enzyme preparation. The difficulty in completely inhibiting PTPase activity in lymphocytes may help explain the variation between laboratories in studies of tyrosine phosphorylation in these cells. Studies with highly purified T lymphocytes obtained by filtration of PBL through nylon wool columns indicated that the activity is present in T cells. Absorption with agarose containing anti-HLe-1, a mouse monoclonal IgG1 antibody specific for the leukocyte-specific surface protein T-200 (CD45), removed up to 40% of the PTPase activity. Enzyme activity was recovered on the immunoadsorbent after extensive washing, confirming that the enzyme was being bound to the beads. Immunoabsorbents containing other mouse IgG1 antibodies failed to bind PTPase activity, indicating that the binding to beads with anti-HLe-1 antibody is specific. Further characterization of the CD45 and PTPase activities in lymphocytes may provide a better understanding of the role of protein tyrosine phosphorylation in the regulation of proliferation and differentiation in these cells.
A stomatin-like protein encoded by the slp gene of Rhizobium etli is required for nodulation competitiveness on the common bean (1998)

Rhizobium etli strain TAL182 is a competitive strain for effective nodulation of beans. From this strain, a novel gene was isolated, slp, which is 669 bp in size and required for nodulation competition on the common bean. The slp knockout mutant of TAL182 is defective in nodulation competition, shows reduced growth in the presence of 200 mM NaCl, KCl or LiCl and is complemented by the cloned slp gene. The deduced amino acid sequence of slp shows 66-72% similarity to stomatin proteins of Homo sapiens, Mus musculus and Caenorhabditis elegans. Expression of slp in Escherichia coli from a T7 promoter shows a 26 kDa protein which cross-reacts with human-stomatin-specific polyclonal antibody. Like the human stomatin protein, the slp-deduced protein, Slp, is very hydrophilic except for a single hydrophobic membrane-spanning domain. Among various bean-nodulating rhizobia, slp is present in R. etli, Rhizobium leguminosarum bv. phaseoli and Rhizobium tropici type A strains but is absent in R. tropici type B strains. It is also absent in Bradyrhizobium and several other Rhizobium spp.
Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone-related peptide gene. (1994)

The parathyroid hormone-related peptide (PTHrP) gene was disrupted in murine embryonic stem cells by homologous recombination, and the null allele was introduced into the mouse germ line. Mice homozygous for the PTHrP null mutation died postnatally, probably from asphyxia, and exhibited widespread abnormalities of endochondral bone development. Histological examination revealed a diminution of chondrocyte proliferation, associated with premature maturation of chondrocytes and accelerated bone formation. Analysis of earlier developmental stages revealed that disturbance in cartilage growth preceded abnormal endochondral bone formation. There were no morphological abnormalities apparent in other tissues. These results provide direct evidence implicating PTHrP in normal skeletal development and serve to emphasize its potential involvement in human osteochondrodysplasias.
Detecting Emails Containing Requests for Action (2010)

Automatically finding email messages that contain requests for action can provide valuable assistance to users who otherwise struggle to give appropriate attention to the actionable tasks in their inbox. As a speech act classification task, however, automatically recognising requests in free text is particularly challenging. The problem is compounded by the fact that typical emails contain extraneous material that makes it difficult to isolate the content that is directed to the recipient of the email message. In this paper, we report on an email classification system which identifies messages containing requests; we then show how, by segmenting the content of email messages into different functional zones and then considering only content in a small number of message zones when detecting requests, we can improve the accuracy of message-level automated request classification to 83.76%, a relative increase of 15.9%. This represents an error reduction of 41 % compared with the same request classifier deployed without email zoning. 1
Three Levels of Networking for Sourcing Intellectual Capital in Biotechnology (1997)

(1997). Three Levels of Networking for Sourcing Intellectual Capital in Biotechnology. International Studies of Management & Organization: Vol. 27, The Construction, Forms, and Consequences of Industry Networks, pp. 76-103.
An Efficient Bayesian PAPR Reduction Method for OFDM-Based Massive MIMO Systems (2016)

We consider the problem of peak-to-average power ratio (PAPR) reduction in orthogonal frequency-division multiplexing (OFDM) based massive multiple-input multiple-output (MIMO) downlink systems. Specifically, given a set of symbol vectors to be transmitted to K users, the problem is to find an OFDM-modulated signal that has a low PAPR and meanwhile enables multiuser interference (MUI) cancellation. Unlike previous works that tackled the problem using convex optimization, we take a Bayesian approach and develop an efficient PAPR reduction method by exploiting the redundant degrees of freedom of the transmit array. The sought-after signal is treated as a random vector with a hierarchical truncated Gaussian mixture prior, which has the potential to encourage a low PAPR signal with most of its samples concentrated on the boundaries. A variational expectation-maximization (EM) strategy is developed to obtain estimates of the hyperparameters associated with the prior model, along with the signal. In addition, the generalized approximate message passing (GAMP) is embedded into the variational EM framework, which results in a significant reduction in computational complexity of the proposed algorithm. Simulation results show our proposed algorithm achieves a substantial performance improvement over existing methods in terms of both the PAPR reduction and computational complexity.
Efficient Image Retrieval Using Adaptive Segmentation of HSV Color Space (2008)

This paper presents an efficient image retrieval system using adaptive segmentation of hue, saturation and value (HSV) color space. We classify the image into n number of areas based on different selected ranges of hue and value, then each area is partitioned into m number of segments based on the number of pixels it contains, the area which has more pixels will be partitioned into more segments and the area which has less pixels will be partitioned into less number of segments. This is used as a feature vector. Retrieval system outputs the image with a high matching factor. A small demonstration system has been tested and shows superior performance compared with the simple color based retrieval systems.
Faecal tumour M2 pyruvate kinase: a new, sensitive screening tool for colorectal cancer (2004)

Proliferating cells, especially tumour cells, express a special isoenzyme of pyruvate kinase, termed M2-PK, which can occur in a tetrameric form with a high affinity to its substrate, phosphoenolpyruvate (PEP), and in a dimeric form with a low PEP affinity. In tumour cells, the dimeric form is usually predominant and is therefore termed Tumour M2-PK. The levels of Tumour M2-PK within tumours and in EDTA-plasma correlate with staging and the ability of the tumour cells to metastasise. Since most colorectal tumours grow intraluminally, it appeared interesting to determine whether Tumour M2-PK is detectable in the faeces of tumour patients. Stool samples were tested by ELISA from controls without colorectal cancer and colorectal cancer patients. Whereas Tumour M2-PK levels were low in the control group (mean value+/-s.e.m.: 3.3+/-0.4, n=144), they were high in the case of colorectal cancer (56.1+/-15.3, n=60). At a cutoff value of 4 U ml(-1), the sensitivity was 73%. TNM and Dukes' classification of the tumours revealed a strong correlation between faecal Tumour M2-PK levels and staging. The determination of Tumour M2-PK in faeces provides a new promising screening tool for colorectal tumours.
Application of a Nested Polymerase Chain Reaction Assay to DetectMycoplasma Hyopneumoniaefrom Nasal Swabs (1999)

The porcine respiratory disease complex (PRDC) is an increasingly important cause of decreased swine productivity and is characterized by slow growth, decreased feed efficiency, anorexia, cough, and dyspnea. Mycoplasma hyopneumoniae is among the most prevalent and important infectious agents associated with PRDC. Understanding of mycoplasmal pneumonia has been hindered by inadequate diagnostic methods. Many of the currently available tests are relatively insensitive or nonspecific when used in a diagnostic laboratory setting or are too costly or difficult for routine diagnostic use. Several polymerase chain reaction (PCR) assays have been described, but they are not sensitive enough to detect the microorganisms in live pigs, from either nasal or tracheal swabs. A nested PCR using 2 species-specific sets of primers from the 16S ribosomal DNA gave positive results with as little as 80 microorganisms and did not cross-react with other mycoplasma species or with other microorganisms commonly found in the respiratory tract of pigs. This assay was better suited for detection of M. hyopneumoniae from nasal swabs than was conventional PCR. Nasal swab samples were taken at different time periods following experimental challenge of 10 susceptible pigs. Only 2 of the 55 swabs examined gave a positive result with conventional PCR, whereas 30 of the 55 swabs gave a positive result using the nested PCR. Twenty of 40 (50%) nasal swabs from pigs experiencing a respiratory disease outbreak where M. hyopneumoniae had been diagnosed also gave a positive result with the nested PCR. To confirm that the amplified product was specific, 4 nested PCR products were purified, sequences were determined and aligned, and they were confirmed to be from M. hyopneumoniae.
The GPR54 Gene as a Regulator of Puberty (2003)

Mutations in GPR54, a G protein-coupled receptor gene, cause autosomal recessive idiopathic hypogonadotropic hypogonadism in humans and mice, suggesting that this receptor is essential for normal gonadotropin-releasing hormone physiology and for puberty.
DEVELOPMENTAL CYTOLOGY OF 13 GENETIC MALE STERILE LOCI IN MAIZE (1981)

Thirteen nonallelic genetic-male sterile loci of maize (Zea mays L.) were investigated cytologically to determine the microsporogenesis breakdown characteristics for each mutant. These male-sterile mutants included ms1, ms2, ms5, ms6, ms7, ms8, ms9, ms10, ms11, ms12, ms13, ms14, and ms17 in A632 and 0h43 inbred backgrounds. Male-sterile mutants ms8 and ms9 resulted in abnormal microspore (pollen) mother cells that exhibited nearly normal nuclear development but abnormal cellular development. These mutants had the earliest effect on microsporogenesis. Male-sterile mutants ms5, ms11, and ms14 had the latest effect on microsporogenesis in that microspores developed until the microspore mitosis stage. Other male-sterile mutants seemed to have similar expressions when compared with each other. Mutants ms2 and ms7 both lacked significant microspore wall formation at the time of microspore collapse. Mutants ms10 and ms13 were similar in that the microspore wall developed to approximately one-half the normal thickness before microspore collapse. A unique feature of ms1 was the occurrence of an abnormally thickened microspore wall. Almost complete microspore wall development occurred in ms12 plants despite nuclear degradation. Mutant ms6 was cytologically and genetically similar to polymitotic (po). Mutant ms17 had variable expression that most notably affected spindle formation. These observations may be useful in utilizing genetic male sterility in maize hybrid seed production schemes.
The effects of harp music in vascular and thoracic surgical patients. (2002)

Subjects in this study experienced decreased pain and anxiety with the harp intervention, and slight reductions in physiologic variable values. It is not possible in this study to determine if the results were due to the harp music, the presence of the harpist and data collector, or both. Future research is recommended using a control group and comparison of live versus recorded harp music with a wider variety of diagnoses and procedures.
Studies on hindered phenols and analogs. 1. Hypolipidemic and hypoglycemic agents with ability to inhibit lipid peroxidation (1989)

A series of hindered phenols were investigated as hypolipidemic and/or hypoglycemic agents with ability to inhibit lipid peroxidation. 1,3-Benzoxathioles (9 and 22), phenoxypentanoic acid (34), phenoxypentanol (35a), phenoxynonanol (35b), phenylchloropropionic acid having a chromanyl group (25), and a thiazolidine compound (27) derived from 25, all having a hindered phenol group, were prepared and examined. Compound 27 showed the expected biological properties in vivo and in vitro without any liver weight increase. Biological activities of the analogous thiazolidine compounds, 43-58, were compared. Thus, (+/-)-5-[4-[(6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)methoxy]- benzyl]-2,4-thiazolidinedione (27) (CS-045) was found to have all of our expected properties and was selected as a candidate for further development as a hypoglycemic and hypolipidemic agent.
Technology management information exchange (TEMINEX). Applied technology assessment for HMOs. (1992)

In an effort to obtain timely, comprehensive and valid information, The HMO Group medical directors have pooled resources to facilitate decision-making on the appropriate use of new or unfamiliar technologies. The HMO Group has responded to over 175 requests for information on topics such as organ transplants, testing and screening procedures, drugs and devices.
Room-Temperature Hiyama Cross-Couplings of Arylsilanes with Alkyl Bromides and Iodides (2003)

The first method for achieving Hiyama couplings of unactivated alkyl bromides and iodides is reported. The desired carbon-carbon bond formation proceeds under mild conditions (room temperature) with good functional-group tolerance.
Kinetics of Energy Flow in the Phycobilisome Core (1985)

Light energy absorbed by the 576 bilin chromophores in the six rods of the phycobilisome of the cyanobacterium Synechocystis 6701 is funneled into a 1.5 x 10(6) dalton core. The 72 bilins of the core function as a single unit with respect to the rate-limiting processes for energy flow within these particles.
On-line adaptive learning of the continuous density hidden Markov model based on approximate recursive Bayes estimate (1997)

We present a framework of quasi-Bayes (QB) learning of the parameters of the continuous density hidden Markov model (CDHMM) with Gaussian mixture state observation densities. The QB formulation is based on the theory of recursive Bayesian inference. The QB algorithm is designed to incrementally update the hyperparameters of the approximate posterior distribution and the CDHMM parameters simultaneously. By further introducing a simple forgetting mechanism to adjust the contribution of previously observed sample utterances, the algorithm is adaptive in nature and capable of performing an online adaptive learning using only the current sample utterance. It can, thus, be used to cope with the time-varying nature of some acoustic and environmental variabilities, including mismatches caused by changing speakers, channels, and transducers. As an example, the QB learning framework is applied to on-line speaker adaptation and its viability is confirmed in a series of comparative experiments using a 26-letter English alphabet vocabulary.
Measurement of the Nanoscale Roughness by Atomic Force Microscopy: Basic Principles and Applications (2012)

Measurement of the Nanoscale Roughness by Atomic Force Microscopy: Basic Principles and Applications
Neuronopathic Gaucher disease in the mouse: viable combined selective saposin C deficiency and mutant glucocerebrosidase (V394L) mice with glucosylsphingosine and glucosylceramide accumulation and progressive neurological deficits (2010)

Gaucher disease is caused by defective acid beta-glucosidase (GCase) function. Saposin C is a lysosomal protein needed for optimal GCase activity. To test the in vivo effects of saposin C on GCase, saposin C deficient mice (C-/-) were backcrossed to point mutated GCase (V394L/V394L) mice. The resultant mice (4L;C*) began to exhibit CNS abnormalities approximately 30 days: first as hindlimb paresis, then progressive tremor and ataxia. Death occurred approximately 48 days due to neurological deficits. Axonal degeneration was evident in brain stem, spinal cord and white matter of cerebellum accompanied by increasing infiltration of the brain stem, cortex and thalamus by CD68 positive microglial cells and activation of astrocytes. Electron microscopy showed inclusion bodies in neuronal processes and degenerating cells. Accumulation of p62 and Lamp2 were prominent in the brain suggesting the impairment of autophagosome/lysosome function. This phenotype was different from either V394L/V394L or C-/- alone. Relative to V394L/V394L mice, 4L;C* mice had diminished GCase protein and activity. Marked increases (20- to 30-fold) of glucosylsphingosine (GS) and moderate elevation (1.5- to 3-fold) of glucosylceramide (GC) were in 4L;C* brains. Visceral tissues had increases of GS and GC, but no storage cells were found. Neuronal cells in thick hippocampal slices from 4L;C* mice had significantly attenuated long-term potentiation, presumably resulting from substrate accumulation. The 4L;C* mouse mimics the CNS phenotype and biochemistry of some type 3 (neuronopathic) variants of Gaucher disease and is a unique model suitable for testing pharmacological chaperone and substrate reduction therapies, and investigating the mechanisms of neuronopathic Gaucher disease.
Universal Parameters for Carbon Nanotube Network-Based Sensors: Can Nanotube Sensors Be Reproducible? (2011)

Carbon nanotube (CNT) network-based sensors have been often considered unsuitable for practical applications due to their unpredictable characteristics. Herein, we report the study of universal parameters which can be used to characterize CNT network-based sensors and make their response predictable. A theoretical model is proposed to explain these parameters, and sensing experiments for mercury (Hg(2+)) and ammonium (NH(4)(+)) ions using CNT network-based sensors were performed to confirm the validity of our model.
Rapid Construction of Capsid-Modified Adenoviral Vectors Through Bacteriophage λ Red Recombination (2004)

There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be time-consuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage lambda Red recombination system as a valuable tool for the easy and rapid construction of capsid-modified adenoviral genomes.
Carotid Plaque MRI and Stroke Risk (2013)

The presence of intraplaque hemorrhage, lipid-rich necrotic core, and thinning/rupture of the fibrous cap on MRI of carotid plaque is associated with increased risk of future stroke or transient ischemic attack in patients with carotid atherosclerotic disease. Dedicated MRI of plaque composition offers stroke risk information beyond measurement of luminal stenosis in carotid atherosclerotic disease.