gebhart/patrepeval huggingface dataset:
An Introduction to Peptide Chemistry (1990)

Amino Acids. Purification and Isolation of Peptides. Amino Acid Analysis. Sequencing. Synthesis. The Structure of LH-RH. Appendices. Index and Abbreviations.
The intranuclear location of simian virus 40 polypeptides VP2 and VP3 depends on a specific amino acid sequence (1987)

A cDNA fragment coding for poliovirus capsid polypeptide VP1 was inserted into a simian virus 40 (SV40) genome in the place of the SV40 VP1 gene and fused in phase to the 3' end of the VP2-VP3 genes. Simian cells were infected with the resulting hybrid virus in the presence of an early SV40 mutant used as a helper. Indirect immunofluorescence analysis of the infected cells using anti-poliovirus VP1 immune serum revealed that the SV40/poliovirus fusion protein was located inside the cell nucleus. Deletions of various lengths were generated in the SV40 VP2-VP3 portion of the hybrid gene using BAL31 nuclease. The resulting virus genomes expressed spliced fusion proteins whose intracellular location was either intranuclear or intracytoplasmic, depending on the presence or absence of VP2 amino acid residues 317 to 323 (Pro-Asn-Lys-Lys-Lys-Arg-Lys). This was confirmed by site-directed mutagenesis of the Lys residue at position 320. Modification of Lys-320 into either Thr or Asn abolished the nuclear accumulation of the fusion protein. It is concluded that at least part of the sequence of VP2 amino acids 317 to 323 allows VP2 and VP3 to remain stably located inside the cell nucleus. The proteins are most probably transported from the cell cytoplasm to the cell nucleus by interaction, with VP1 acting as a carrier.
Fibrosis and disease progression in hepatitis C (2002)

The progression of fibrosis in chronic hepatitis C determines the ultimate prognosis and thus the need and urgency of therapy. Fibrogenesis is a complex dynamic process, which is mediated by necroinflammation and activation of stellate cells. The liver biopsy remains the gold standard to assess fibrosis. Scoring systems allow a semiquantitative assessment and are useful for cross-sectional and cohort studies and in treatment trials. The rate at which fibrosis progresses varies markedly between patients. The major factors known to be associated with fibrosis progression are older age at infection, male gender, and excessive alcohol consumption. Viral load and genotype do not seem to influence significantly the progression rate. Progression of fibrosis is more rapid in immunocompromised patients. Hepatic steatosis, obesity, and diabetes may also contribute to more rapid progression of fibrosis. There are no tests that reliably predict the rate of progression of fibrosis in an individual patient. High serum alanine aminotransferase (ALT) levels are associated with a higher risk of fibrosis progression, and worsening of fibrosis is uncommon in patients with persistently normal serum aminotransferase levels. Serum markers for fibrosis are not reliable and need to be improved and validated. Liver biopsy provides the most accurate information on the stage of fibrosis and grade of necroinflammation, both of which have prognostic significance. Repeating the liver biopsy, 3 to 5 years after an initial biopsy is the most accurate means of assessing the progression of fibrosis.
Isolation and Structure of Eucosterol and 16 β‐Hydroxyeucosterol, Two Novel Spirocyclic Nortriterpenes, and of a New 24‐ Nor‐5α‐chola‐8, 16‐diene‐23‐oic Acid from Bulbs of Several Eucomis Species (1976)

Abstract Eucosterol and 16 β‐hydroxy‐eucosterol which have been isolated from several Eucomis species have been shown to be (23 S )‐17,23‐epoxy‐3β,31‐dihydroxy‐27‐nor‐5α‐lanost‐8‐ene‐15,24‐dione ( 1 ) and (23 S )‐17,23‐epoxy‐3β,16β,31‐trihydroxy‐27‐nor‐5α‐lanost‐8‐ene‐15,24‐dione ( 2 ) by chemical transformations and spectral data. The spiro‐fused furanoic ether linkage of both metabolites represents a novel structural element for natural nortriterpenes. The structure of another metabolite ( 16 ), 3β‐hydroxy‐4β‐hydroxymethyl‐4,14α‐dimethyl‐15‐oxo‐24‐nor‐5α‐chola‐8,16‐diene‐23‐oic acid, from Eucomis autumnalis (Mill.) Chitt. was elucidated by chemical correlation of its methyl ester 17 with a degradation product of eucosterol ( 1 ).
Therepeutic future direction with new clinical trials for refractory lymphoid malignancies. (1997)

There are some refractory lymphomas those 5-year overall survival rate is less than 50%, including relapsed or chemotherapy-resistant aggressive NHL, high-intermediate-, or high-risk group of aggressive NHL categorized by International Prognostic Index, mantle cell lymphoma, advanced NK/T cell lymphoma, adult T-cell leukemia/lymphoma (ATL) and advanced and refractory follicular lymphoma. We report here the results of three clinical trials for these refractory lymphomas, as follows; (1) phase I/II trial of cure-oriented high-dose chemoradiotherapy with transplantation of CD34+ peripheral blood stem cells purified by the immunomagnetic bead method for refractory hematological malignancies. (2) phase I study of cladribine (2-chlorodeoxyadenosine) in lymphoid malignancies. (3) clinical trial of chimeric anti-CD20 monoclonal antibody (IDEC-C2B8) in patients with recurrent B-cell lymphoma.(1) Nine patients with relapsed or refractory hematological malignancies including 8 NHL patients were enrolled and treated by high-dose chemoradiotherapy with transplantation of CD34+ peripheral blood stem cells purified by the immunomagnetic bead method. In each patient, a median of 1.75×106cells/kg purified CD34+ PBSCs (purity; 92%) were infused. Median times to hematopoietic recovery were as same as those in non-purged PBSCT. Most patients experienced only transient toxicities previously observed with the preparative chemotherapy regimens used. Seven patients are alive in remission. This phase I/II trial showed PBSCT with the immunomagnetic bead method to be an active and safe therapy. (2) To clarify the toxicity profiles of cladribine, we conducted a phase I and pharmacological study of cladribine with a schedule of seven-day continuous intravenous infusion every 28 days up to a maximun of three cycles. Anti-tumor effect was observed in the patients with ATL, cutaneous T-cell lymphoma and mantle cell lymphoma. The encouraging results prompted us to plan subsequent phase II studies of cladribine against ATL, hairy cell leukemia and indolent lymphoma. (3) A phase I/II trial of a chimeric mouse human monoclonal antibody to CD20 for CD20 positive recurrent indolent B-cell lymphoma in USA showed a response rate of 50%. A phase II trial has been started for recurrent or relapsed mantle cell lymphoma and indolent B-cell lymphoma in Japan. These three clical trials showed that CD34+ PBSCT by the immunomagnetic bead method, cladribine and IDEC-C2B8 are safe and active therapy or drugs, and suggested promising method or agents for refractory lymphomas.
Levels of Alpha-Fetoprotein During Pregnancy and Early Infancy in Normal and Disease States (2003)

Alpha-fetoprotein (AFP) was 1 of the first serum protein markers to serve in the dual capacities of tumor marker and fetal defect marker, ie, an oncofetal protein, in the clinical laboratory. Although the serum-marker capacity of AFP has long been used, less is known of the fluid compartments of this oncofetal protein during fetal and perinatal development. In this review, the biologic activities of AFP are discussed in light of its presence in the various biologic fluid compartments: fetal serum, amniotic fluid, cord blood, urine, and maternal serum. AFP concentrations within the biologic fluids are considered in the context of gestational age, sex, body weight, and anatomic location. Discussion follows concerning the relationships and roles of AFP in various developmental disorders such as hypothyroidism, folate deficiencies, autoimmune disorders, acquired immunodeficiency disorder (AIDS), congenital heart defects, cystic fibrosis, preeclampsia/hypertension, and platelet aggregation disorders. Based on its presence in so many types of birth defects, malformations, and congenital anomalies, AFP can be seen to serve as a form of molecular "duct tape" during pregnancy and postnatal development.
Allogeneic Cardiospheres Delivered via Percutaneous Transendocardial Injection Increase Viable Myocardium, Decrease Scar Size, and Attenuate Cardiac Dilatation in Porcine Ischemic Cardiomyopathy (2014)

Dose-optimized injection of allogeneic cardiospheres is safe, decreases scar size, increases viable myocardium, and attenuates cardiac dilatation in porcine chronic ischemic cardiomyopathy. The decreases in scar size, mirrored by increases in viable myocardium, are consistent with therapeutic regeneration.
Visual pattern recognition by moment invariants (1962)

In this paper a theory of two-dimensional moment invariants for planar geometric figures is presented. A fundamental theorem is established to relate such moment invariants to the well-known algebraic invariants. Complete systems of moment invariants under translation, similitude and orthogonal transformations are derived. Some moment invariants under general two-dimensional linear transformations are also included. Both theoretical formulation and practical models of visual pattern recognition based upon these moment invariants are discussed. A simple simulation program together with its performance are also presented. It is shown that recognition of geometrical patterns and alphabetical characters independently of position, size and orientation can be accomplished. It is also indicated that generalization is possible to include invariance with parallel projection.
NS5A - From Obscurity to New Target for HCV Therapy (2008)

The hepatitis C virus (HCV) non-structural 5A (NS5A) protein is essential for viral RNA replication and may play a role in subverting host intracellular signaling pathways. Although no intrinsic enzymatic activity has been ascribed to NS5A, this proline-rich hydrophilic phosphoprotein is likely to exert its functions by interacting with viral and cellular factors. Recent studies using the HCV replicon cell culture system as a model for HCV RNA replication as well as for high-throughput screening of pharmacological inhibitors have revealed blockade of NS5A as a promising therapeutic strategy for the treatment of HCV. This review will summarize our progress in understanding the role of NS5A in HCV RNA replication and will introduce the most recent patents on inhibitors of NS5A.
Another Generalization of Resolution (1978)

article Free Access Share on Another Generalization of Resolution Authors: Norman Rubin Polytechnic Institute of New York, 230 Jay Street, Brooklyn, NY and New York University, New York, New York Polytechnic Institute of New York, 230 Jay Street, Brooklyn, NY and New York University, New York, New YorkView Profile , Malcolm C. Harrison Courant Institute of Mathematical Sciences, New York University, 251 Mercer Street, New York, NY Courant Institute of Mathematical Sciences, New York University, 251 Mercer Street, New York, NYView Profile Authors Info & Claims Journal of the ACMVolume 25Issue 3July 1978 pp 341–351https://doi.org/10.1145/322077.322078Published:01 July 1978Publication History 12citation294DownloadsMetricsTotal Citations12Total Downloads294Last 12 Months3Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
PURIFIED HUMAN PLASMA PROTEINS OF UNKNOWN FUNCTION (1981)

The development of new analytic and preparative techniques in the field of protein chemistry has essentially extended our knowledge about the variety of human plasma proteins in the last 15 years. In many cases plasma proteins have been particularly determined by immunologic techniques, partially highly purified and physicochemically well characterized, before knowing the biological function. To these proteins belong among others the alpha 1-antitrypsin, Cl-inactivator, alpha 2-macroglobulin, Gc-globulin and the cold-insoluble globulin. Today we know more than 100 proteins being isolated from human plasma and among these are nearly 20, of which the biological function is not yet known. To this group are belonging proteins, which are known since many years, like the alpha 1-acid glycoprotein and the C-reactive proteins as well as proteins, which have only been described in the last years and which partially have an interesting chemical structure, e.g. the histidinerich 3,8S-alpha 2-glycoprotein and the leucine-rich 3,1S-alpha 2-glycoprotein, of which every fifth amino acid is formed by leucine. It is to be hoped that the special chemical structure of some of these human plasma proteins as well as the quantitative immunologic determination in different patient sera will give hints to their biological function.
Rapid Genetic Selection of Inhibitor-Resistant Protease Mutants: Clinically Relevant and Novel Mutants of the HIV Protease (2001)

Site-specific proteolysis is an important regulatory mechanism in many basic cellular processes as well as playing critical roles in the life cycle of viruses and other pathogenic organisms. For the human immunodeficiency virus (HIV) the encoded protease is required for the replication of the virus and has been the target of novel antiviral therapeutics. However, the emergence of inhibitor-resistant viral strains has become an increasingly significant clinical problem. Using a bacteriophage-based genetic selection, a bank of inhibitor-resistant mutants in this protease has been isolated that includes mutations that correlate with resistant clinical isolates. The rapid selection of such mutations has implications for the prediction of relevant mutations and may be applicable to other viral systems.
BID: a novel BH3 domain-only death agonist. (1996)

The BCL-2 family of proteins consists of both antagonists (e.g., BCL-2) and agonists (e.g., BAX) that regulate apoptosis and compete through dimerization. The BH1 and BH2 domains of BCL-2 are required to heterodimerize with BAX and to repress cell death; conversely, the BH3 domain of BAX is required to heterodimerize with BCL-2 and to promote cell death. To extend this pathway, we used interactive cloning to identify Bid, which encodes a novel death agonist that heterodimerizes with either agonists (BAX) or antagonists (BCL-2). BID possesses only the BH3 domain, lacks a carboxy-terminal signal-anchor segment, and is found in both cytosolic and membrane locations. BID counters the protective effect of BCL-2. Moreover, expression of BID, without another death stimulus, induces ICE-like proteases and apoptosis. Mutagenesis revealed that an intact BH3 domain of BID was required to bind the BH1 domain of either BCL-2 or BAX. A BH3 mutant of BID that still heterodimerized with BCL-2 failed to promote apoptosis, dissociating these activities. In contrast, the only BID BH3 mutant that retained death promoting activity interacted with BAX, but not BCL-2. This BH3-only molecule supports BH3 as a death domain and favors a model in which BID represents a death ligand for the membrane-bound receptor BAX.
Functional Analysis of a 450–Amino Acid N-Terminal Fragment of Phytochrome B in Arabidopsis (2004)

Phytochrome, a major photoreceptor in plants, consists of two domains: the N-terminal photosensory domain and the C-terminal domain. Recently, the 651-amino acid photosensory domain of phytochrome B (phyB) has been shown to act as a functional photoreceptor in the nucleus. The phytochrome (PHY) domain, which is located at the C-terminal end of the photosensory domain, is required for the spectral integrity of phytochrome; however, little is known about the signal transduction activity of this domain. Here, we have established transgenic Arabidopsis thaliana lines expressing an N-terminal 450-amino acid fragment of phyB (N450) lacking the PHY domain on a phyB-deficient background. Analysis of these plants revealed that N450 can act as an active photoreceptor when attached to a short nuclear localization signal and beta-glucuronidase. In vitro spectral analysis of reconstituted chromopeptides further indicated that the stability of the N450 Pfr form, an active form of phytochrome, is markedly reduced in comparison with the Pfr form of full-length phyB. Consistent with this, plants expressing N450 failed to respond to intermittent light applied at long intervals, indicating that N450 Pfr is short-lived in vivo. Taken together, our findings show that the PHY domain is dispensable for phyB signal transduction but is required for stabilizing the Pfr form of phyB.
Novel Short Oligonucleotide Conjugates as Inhibitors of Human Telomerase (2003)

A series of oligonucleotide conjugates were designed and synthesized as novel inhibitors of human telomerase. These compounds contain a relatively short (6-7-mer) oligonucleotide domain, with an N3'-->P5' phosphoramidate (np) or thio-phosphoramidate (nps) backbone, targeted to the template region of the RNA component of the enzyme and various pendant groups attached to either their 5'- or preferably to the 3'-termini. The most potent compounds in the series inhibited telomerase with low nM IC50 values in biochemical assays whereas the cognate oligonucleotides without the pendant groups were significantly less active having IC50 values 100-1000-fold higher.
GaAs integrated optical circuits by wet chemical etching (1979)

An integrated optical circuit containing a laser, passive waveguide, and extra-cavity detector is described. These devices are fabricated from Al <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</inf> Ga <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1-x</inf> As layers grown by liquid-phase epitaxy. Reflectors are formed by a two-step preferential etch procedure. For proper laser orientation on {100} wafers, the mirrors are found to be oblique, tapered so as to enhance coupling into the underlying passive waveguide; this increases the efficiency of radiation transfer to the detector. Device operation with high differential transfer efficiency and low threshold has been achieved; e.g., for devices with one etched mirror and one cleaved mirror, η <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</inf> = 16 percent and <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">J_{t} = 2.4</tex> kA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , whereas these values are <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\eta_{t} = 6.5</tex> percent and J <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</inf> = 3.0 kA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> for devices with two etched mirrors. Other orientations on {100} and {110} wafers have also been investigated. The reflectivity of the etched mirrors is small, <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">R_{e} \simeq 1-2</tex> percent, but transfer efficiencies into the external passive waveguide as large as <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T = 50</tex> percent have been observed. The effect of small R <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">e</inf> on device performance is examined both experimentally and theoretically. The fabrication of ribbed interconnections between active circuit components is also described.
IL-2-induced expression of TTK, a serine, threonine, tyrosine kinase, correlates with cell cycle progression. (1994)

We have recently isolated the cDNA for a unique human 97-kDa kinase, TTK, by expression screening of a cDNA expression library using anti-phosphotyrosine antibodies. When expressed in Escherichia coli, TTK can phosphorylate serine, threonine, and tyrosine residues. Thus TTK appears to belong to a newly described family of kinases able to phosphorylate all three hydroxy amino acids. This family of multispecific kinases includes several other kinases involved in cell cycle progression. In support of a possible role in regulating cell cycle progression, TTK message is readily detected in rapidly proliferating tissues in vivo including testes, thymus, bone marrow, and many malignant tumors, but not in benign tissues with a low proliferative rate in vivo. To determine the effect of cell activation and cell cycle progression on TTK expression, we measured TTK mRNA and protein levels as well as kinase activity in freshly isolated T cells or IL-2-expanded T cell blasts activated to proliferate by the addition of a variety of mitogens. TTK mRNA levels, protein levels, and kinase activity were greatly enhanced when either freshly isolated PBL or T cell blasts were activated by cross-linking the TCR complex by mitogenic lectins or by bypassing the TCR with phorbol esters and cation ionophores. Incubation with IL-2 increased TTK expression in PBL blasts, which proliferate in response to IL-2, but not in fresh PBL, which do not proliferate in response to IL-2. TTK expression was blocked by either cyclosporin A or FK520, which inhibit IL-2 production and could be recovered by the addition of exogenous IL-2. Furthermore, TTK expression was prevented by incubation of the cells with rapamycin, which blocks IL-2 signaling. Thus, TTK expression in T cells appears to be a consequence of IL2-induced cell proliferation. Agonist-induced TTK expression was a delayed event occurring 12 to 24 h after activation of PBL blasts and 48 to 72 h after activation of fresh PBL. TTK protein and mRNA expression increased in both fresh PBL and T cell blasts concurrently with passage of cells through S phase as indicated by [3H]TdR incorporation and cell cycle analysis of propidium iodide-stained cells. TTK mRNA and protein levels reached a maximum as cells entered the G2 phase of the cell cycle. These results were confirmed by cell cycle blockade studies with aphidicolin and nocodazole wherein TTK protein levels are not detected in cells in G1 and are readily detectable in cells in the S and G2 phases of the cell cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
From documents to objects (1998)

article Free Access Share on From documents to objects: an overview of LiveDoc Authors: James R. Miller View Profile , Thomas Bonura View Profile Authors Info & Claims ACM SIGCHI BulletinVolume 30Issue 2April 1998 pp 53–58https://doi.org/10.1145/279044.279155Published:01 April 1998Publication History 4citation73DownloadsMetricsTotal Citations4Total Downloads73Last 12 Months14Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Trichlorosilane-Dimethylformamide (Cl3SiH-DMF) as an Efficient Reducing Agent. Reduction of Aldehydes and Imines and Reductive Amination of Aldehydes under Mild Conditions Using Hypervalent Hydridosilicates (1996)

Abstract Trichlorosilane-dimethylformamide (Cl3SiH-DMF) was found to be an effective reducing agent for reduction of aldehydes to alcohols, imines to amines, and also reductive amination of aldehydes. Hypervalent silicates are active species, which enable efficient reduction under mild conditions.