gebhart/patrepeval huggingface dataset:
Induced stereo- and substrate selectivity of bioimprinted .alpha.-chymotrypsin in anhydrous organic media (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInduced stereo- and substrate selectivity of bioimprinted .alpha.-chymotrypsin in anhydrous organic mediaMarianne Staahl, Ulla Jeppsson-Wistrand, Mats Olle Maansson, and Klaus MosbachCite this: J. Am. Chem. Soc. 1991, 113, 24, 9366–9368Publication Date (Print):November 1, 1991Publication History Published online1 May 2002Published inissue 1 November 1991https://pubs.acs.org/doi/10.1021/ja00024a051https://doi.org/10.1021/ja00024a051research-articleACS PublicationsRequest reuse permissionsArticle Views311Altmetric-Citations102LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Fat splitting (1979)

Abstract Fat splitting, particularly the continuous, high pressure, countercurrent hydrolysis of fats and oils, typified by the Colgate‐Emery or modified processes, represents the technological cornerstone for today's American fatty acid industry. Internationally, other methods such as Twitchell or batch autoclave “medium‐pressure” catalyzed or uncatalyzed splitting are still important. All industrial fat splitting methods have as their objectives the attainment of a high rate of hydrolysis together with a high degree of completeness. This objective is achieved, more or less, by the proper optimum balance of: (a) use of excess water; (b) selection of appropriate combination of temperature and pressure to optimize the solubility of liquid water in the fat phases with or without use of suitable “water‐in‐oil” emulsifiers; (c) use or nonuse of acidic catalyst (rarely basic catalysts); and (d) removal of byproduct glycerol. Significant conditions and details in fat splitting by the important commercial processes are described.
[125I]‐(Pyr1)Apelin‐13 is a novel radioligand for localizing the APJ orphan receptor in human and rat tissues with evidence for a vasoconstrictor role in man (2001)

1. We have determined the binding characteristics of [(125)I]-(Pyr(1))Apelin-13, a putative ligand for the APJ orphan receptor in human cardiovascular and rat tissue and investigated the functional properties of (Pyr(1))Apelin-13 in human saphenous vein. 2. The binding of [(125)I]-(Pyr(1))Apelin-13 to sections of human heart tissue was time dependent and rapid at 23 degrees C. Data were fitted to a single site model with an association rate constant (k(obs)) of 0.115 min(-1). [(125)I]-(Pyr(1))Apelin-13 also dissociated from a single site with a dissociation rate constant of 0.0105 min(-1). 3. In saturation binding experiments [(125)I]-(Pyr(1))Apelin-13 bound to human left ventricle with a K(D) value of 0.35+/-0.08 nM, B(max) of 4.3+/-0.9 fmol mg(-1) protein with a Hill slope of 0.97+/-0.04 and to the right atria with a K(D) of 0.33+/-0.09 nM, B(max) of 3.1+/-0.6 fmol mg(-1) protein and a Hill slope of 0.93+/-0.05. 4. [(125)I]-(Pyr(1))Apelin-13 binding sites were localized using autoradiography to human cardiovascular tissue, including coronary artery, aorta and saphenous vein grafts. In rat tissue a high density of receptors were localized to the molecular layer of the rat cerebellum, rat lung, rat heart and low levels in the rat kidney cortex. 2. (Pyr(1))Apelin-13 potently contracted human saphenous vein with a pD(2) value of 8.4+/-0.2 (n=8). The maximum response elicited by the peptide was 22.6+/-6% of 100 mM KCl. 6. We provide the first evidence of APJ receptor expression, relative densities and functional properties of (Pyr(1))Apelin-13 in human cardiovascular tissue.
Self assembled nanoparticle aggregates from line focused femtosecond laser ablation (2010)

In this paper we present the use of a line focused femtosecond laser beam that is rastered across a 2024 T3 aluminum surface to produce nanoparticles that self assemble into 5-60 micron diameter domed and in some cases sphere-shaped aggregate structures. Each time the laser is rastered over initial aggregates their diameter increases as new layers of nanoparticles self assemble on the surface. The aggregates are thus composed of layers of particles forming discrete layered shells inside of them. When micron size aggregates are removed, using an ultrasonic bath, rings are revealed that have been permanently formed in the sample surface. These rings appear underneath, and extend beyond the physical boundary of the aggregates. The surface is blackened by the formation of these structures and exhibits high light absorption.
How the Solute Polydispersity Affects the Cloud-Point and Coexistence Pressures in Propylene and Ethylene Solutions of Alternating Poly(ethylene-co-propylene) (1997)

Cloud-point and coexistence pressures, sub- and supercritical, are determined experimentally for a series of ethylene and propylene solutions of nearly monodisperse and polydisperse poly(ethylene-co-propylene), referred to as PEP. The cloud-point and coexistence pressures are found to be close for solutions of nearly monodisperse PEP polymers but not for solutions of polydisperse PEP. The SAFT parameters derived from the experimental cloud points for nearly monodisperse solutes alone are found to predict the cloud-point pressures and solubilities for polydisperse PEP as well.
Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2‐T1R3 receptor (2002)

Sweet tasting proteins interact with the same receptor that binds small molecular weight sweeteners, the T1R2-T1R3 G-protein coupled receptor, but the key groups on the protein surface responsible for the biological activity have not yet been identified. I propose that sweet proteins, contrary to small ligands, do not bind to the 'glutamate-like' pocket but stabilize the free form II of the T1R2-T1R3 receptor by attachment to a secondary binding site. Docking of brazzein, monellin and thaumatin with a model of the T1R2-T1R3 sweet taste receptor shows that the most likely complexes can indeed stabilize the active form of the receptor.
Predicting olfactory receptor neuron responses from odorant structure (2007)

The chemical meaning of molecular descriptors helps understand structure-response relationships for olfactory receptors and their "receptive fields". Moreover, it is possible to predict receptor neuron activation from chemical structure using machine-learning techniques, although this is still complicated by a lack of training data.
Determination of the oxidative stability of fats and oils: Comparison between the active oxygen method (AOCS Cd 12‐57) and the rancimat method (1986)

Abstract Oxidative stability is an important parameter in the characterization of fats and oils. The determination of this parameter with the Active Oxygen Method (AOM; AOCS Method Cd 12‐57) is both very costly and labor intensive, owing to the repeated peroxide value determinations involved. The alternative rancimat method is based on the conductometric determination of volatile degradation products and features automatic plotting of the conductivity against time. The evaluation is performed graphically after completion of the experiment. The labor required for this method is considerably less as it is not necessary to perform titrations at regular intervals. In the determination of the peroxide values of six samples at three temperatures, ca 151 mixed solvent and chemicals valued at SFr. 400 (ca $180 US) were consumed. The induction times (t I ) determined with both methods using six different fats and oils show a good correlation (slope 1.005, correlation coefficient 0.987). The rancimat method thus yields results equivalent to the AOCS Method Cd 12–57, but offers a real alternative for the determination of oxidative stabilities owing to the appreciable saving in labor.
Bifurcated traffic and channel assignment (BITCA) to interconnected metro rings (2002)

We have proposed a bifurcated approach to channel assignment in interconnected metro-rings and are able to reduce the net kind of transponder cards needed for dynamic traffic requests. We have compared different tunable cards and estimated a relation for economical optimized tunability for a given number of wavelengths and see that the optimized transponder need not have tunability greater than /spl lambda//sub max//10 for optimized throughput and blocking probability.
Asymmetric Claisen rearrangement catalyzed by chiral organoaluminum reagent (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric Claisen rearrangement catalyzed by chiral organoaluminum reagentKeiji Maruoka, Hiroshi Banno, and Hisashi YamamotoCite this: J. Am. Chem. Soc. 1990, 112, 21, 7791–7793Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October 1990https://pubs.acs.org/doi/10.1021/ja00177a047https://doi.org/10.1021/ja00177a047research-articleACS PublicationsRequest reuse permissionsArticle Views1158Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice (1998)

Receptor tyrosine kinases Flt-1 and Flk-1/KDR, and their ligand, the vascular endothelial growth factor (VEGF), were shown to be essential for angiogenesis in the mouse embryo by gene targeting. Flk-1/KDR null mutant mice exhibited impaired endothelial and hematopoietic cell development. On the other hand, Flt-1 null mutation resulted in early embryonic death at embryonic day 8.5, showing disorganization of blood vessels, such as overgrowth of endothelial cells. Flt-1 differs from Flk-1 in that it displays a higher affinity for VEGF but lower kinase activity, suggesting the importance of its extracellular domain. To examine the biological role of Flt-1 in embryonic development and vascular formation, we deleted the kinase domain without affecting the ligand binding region. Flt-1 tyrosine kinase-deficient homozygous mice (flt-1(TK-/-)) developed normal vessels and survived. However, VEGF-induced macrophage migration was strongly suppressed in flt-1(TK-/-) mice. These results indicate that Flt-1 without tyrosine kinase domain is sufficient to allow embryonic development with normal angiogenesis, and that a receptor tyrosine kinase plays a main biological role as a ligand-binding molecule.
Gene therapy of gliomas: receptor and transcriptional targeting. (1998)

Through incremental increases in the overall therapeutic ratio of combined modality regimens, each addition of unique selective toxicity to a tumor moves one step closer to a cure. The primary advantage of adding gene therapy strategies to current oncologic regimens is the ability to design multiple levels of unique biologic selectivity into vectors using recombinant technology. This article presents an overview of current and potential methods for designing vectors targeted to high grade gliomas through selective cell entry or transcriptional regulation. Cell entry based methodologies are founded on increasing relative uptake of the vector through the chemical or recombinant addition of epitopes which bind to receptors selectively expressed on target cells. Transcriptional targeting utilizes promoter and enhancer systems which have potential for selectively activating transcription for transgene expression or vector propagation in target cells.
Producing “Self-Plasticizing” Ion-Selective Membranes (1999)

Polymer membranes have been explored for the analysis of ions that do not require plasticizers and with photocurable properties. This work was focused on investigating the viability of the methacrylic-acrylic copolymers as new self-plasticizing membrane matrixes for ion-selective electrodes or other ion-sensor applications. Copolymers with glass transition temperatures ranging from -20 to -44 degrees C could be prepared without added plasticizer and were found to be functional as ion-selective membranes. Both free-radical solution polymerization and photopolymerization could be used, and "self-plasticizing" behavior of copolymers was observed with a high alkylacrylate (R = C4) content. This was found to be compatible with most commercially available ionophores, and sensors for potassium, sodium, calcium and pH were fabricated entirely by photocure procedures; single-step procedures for the immobilization of benzo-15-crown-5 ionophore on these self-plasticizing copolymer matrixes were also developed. Even though the ionophore was immobilized, potentiometric studies revealed that the ionophore remained functional, and thus, these copolymers have the advantage of suffering neither leaching of ionophore nor plasticizers. All these sensors exhibited a Nernstian or near Nernstian response with selectivity comparable to plasticized PVC membranes or other plasticized and photocurable polymer membranes. The long-term response of the potassium sensor with immobilized ionophore and the sodium sensor showed little deterioration for as long as one month and three months, respectively, under continuous use.
Cathode Material with Nanorod Structure—An Application for Advanced High-Energy and Safe Lithium Batteries (2013)

We have developed a novel cathode material based on lithium–nickel–manganese–cobalt oxide, where the manganese concentration remains constant throughout the particle, while the nickel concentration decreases linearly and the cobalt concentration increases from the center to the outer surface of the particle. This full concentration gradient material with a fixed manganese composition (FCG–Mn-F) has an average composition of Li[Ni0.60Co0.15Mn0.25]O2 and is composed of rod-shaped primary particles whose length reaches 2.5 μm, growing in the radial direction. In cell tests, the FCG–Mn-F material delivered a high capacity of 206 mAh g–1 with excellent capacity retention of 70.3% after 1000 cycles at 55 °C. This cathode material also exhibited outstanding rate capability, good low-temperature performance, and excellent safety, compared to a conventional cathode having the same composition (Li[Ni0.60Co0.15Mn0.25]O2), where the concentration of the metals is constant across the particles.
A simplified method for the preparation of detergent-free lipid rafts (2005)

Lipid rafts are small plasma membrane domains that contain high levels of cholesterol and sphingolipids. Traditional methods for the biochemical isolation of lipid rafts involve the extraction of cells with nonionic detergents followed by the separation of a low-density, detergent-resistant membrane fraction on density gradients. Because of concerns regarding the possible introduction of artifacts through the use of detergents, it is important to develop procedures for the isolation of lipid rafts that do not involve detergent extraction. We report here a simplified method for the purification of detergent-free lipid rafts that requires only one short density gradient centrifugation, but yields a membrane fraction that is highly enriched in cholesterol and protein markers of lipid rafts, with no contamination from nonraft plasma membrane or intracellular membranes.
Twilight zone of protein sequence alignments (1999)

Sequence alignments unambiguously distinguish between protein pairs of similar and non-similar structure when the pairwise sequence identity is high (>40% for long alignments). The signal gets blurred in the twilight zone of 20-35% sequence identity. Here, more than a million sequence alignments were analysed between protein pairs of known structures to re-define a line distinguishing between true and false positives for low levels of similarity. Four results stood out. (i) The transition from the safe zone of sequence alignment into the twilight zone is described by an explosion of false negatives. More than 95% of all pairs detected in the twilight zone had different structures. More precisely, above a cut-off roughly corresponding to 30% sequence identity, 90% of the pairs were homologous; below 25% less than 10% were. (ii) Whether or not sequence homology implied structural identity depended crucially on the alignment length. For example, if 10 residues were similar in an alignment of length 16 (>60%), structural similarity could not be inferred. (iii) The 'more similar than identical' rule (discarding all pairs for which percentage similarity was lower than percentage identity) reduced false positives significantly. (iv) Using intermediate sequences for finding links between more distant families was almost as successful: pairs were predicted to be homologous when the respective sequence families had proteins in common. All findings are applicable to automatic database searches.
Small Molecule Modulators of Protein–Protein Interactions: Selected Case Studies (2014)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTSmall Molecule Modulators of Protein–Protein Interactions: Selected Case StudiesMadhu Aeluri, Srinivas Chamakuri, Bhanudas Dasari, Shiva Krishna Reddy Guduru, Ravikumar Jimmidi, Srinivas Jogula, and Prabhat Arya*View Author Information Dr. Reddy's Institute of Life Sciences (DRILS), University of Hyderabad Campus Gachibowli, Hyderabad 500046, India*Phone: (+91) (40) 6657 1500. E mail: [email protected]Cite this: Chem. Rev. 2014, 114, 9, 4640–4694Publication Date (Web):March 27, 2014Publication History Received25 July 2013Published online27 March 2014Published inissue 14 May 2014https://pubs.acs.org/doi/10.1021/cr4004049https://doi.org/10.1021/cr4004049review-articleACS PublicationsCopyright © 2014 American Chemical SocietyRequest reuse permissionsArticle Views6379Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Apoptosis,Cells,Inhibitors,Peptides and proteins,Small molecules Get e-Alerts
Development of advanced 3D chip stacking technology with ultra-fine interconnection (2002)

The development of 3D chip stacking technology with ultra-fine pitch interconnection initiated in 1999, which is a part of "Ultra High-Density Electronic System Integration" project. The development involves the backend of wafer fabrication process, packaging and testing. The extended study revealed possibilities and advantages of 3D chip stacking structure with ultra-fine interconnection.
Expression patterns of CEACAM5 and CEACAM6 in primary and metastatic cancers (2007)

CEACAM6 expression is elevated in many solid tumors, but variable as a function of histotype. Based on previous work demonstrating a role for CEACAM6 in tumor cell migration, invasion and adhesion, and formation of distant metastases (Blumenthal et al., Cancer Res 65: 8809-8817, 2005), it may be a promising target for antibody-based therapy.
High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry (2006)

MIMS makes it possible for the first time to both image and quantify molecules labeled with stable or radioactive isotopes within subcellular compartments.
Chloroplast Import of Four Carotenoid Biosynthetic Enzymes In Vitro Reveals Differential Fates Prior to Membrane Binding and Oligomeric Assembly (1997)

The precursor proteins of the carotenogenic enzymes geranylgeranyl diphosphate synthase, phytoene synthase, phytoene desaturase and lycopene cyclase were imported into isolated pea chloroplasts. Geranylgeranyl diphosphate synthase remained soluble in the stroma in a free form and phytoene synthase associated to thylakoid membranes upon import, both as expected. Surprisingly, phytoene desaturase and lycopene cyclase, which strongly depend on membrane association for enzymatic activity, also remained soluble in the chloroplast stroma. The soluble forms of these enzymes were, however, still competent for membrane-association, e.g. with protein-free liposomal membranes. Indeed the soluble forms of phytoene synthase, phytoene desaturase and lycopene cyclase occurred as ATP- and cold-sensitive high-molecular-mass complexes. Gel-filtration experiments and blue native-PAGE plus autoradiography and western blot analysis indicated a participation of the chloroplast 60-kDa chaperonin (Cpn60) in the soluble high-molecular-mass complexes of imported carotenogenic enzymes. Finally, it was inferred that a membrane-bound regulatory factor plays a decisive role in membrane-binding.
S-106 (2012)

Sacks, Jason D.; Rappold, Ana G.; Davis, J. Allen; Richardson, David B.; Waller, Anna E.; Luben, Thomas J. Author Information