gebhart/patrepeval huggingface dataset:
A systematic review of the histological and molecular changes in rotator cuff disease (2012)

The pathogenesis of RCD is being slowly unravelled as a result of the significant recent advances in molecular medicine. Future research aimed at further unlocking these key molecular processes will be pivotal in developing new surgical interventions both in terms of the diagnosis and treatment of RCD.
Teachers’ Grading Decision Making: Multiple Influencing Factors and Methods (2015)

This study investigated Chinese secondary school English language teachers’ grading decision making, focusing on the factors they considered and types of assessment they used for grading. A questionnaire was issued to 350 secondary school English language teachers in China. Descriptive analyses of the questionnaire data showed that these teachers of English considered achievement and non-achievement factors in grading, placing greater weight on non-achievement factors, such as effort, homework, and study habits, and that they used multiple types of assessment, including performance and project-based assessment, teacher self-developed assessment, as well as paper and pencil tests for grading. MANOVA results suggested that both internal and external factors, such as the grade level teachers teach, the assessment training they have received, and their class size affect different aspects of their grading decision making. Multiple regression results further showed a significant relationship between the factors teachers considered and the types of assessment they used for grading. This study contributes to the understanding of the classroom English language teachers’ grading decision making in general and especially in the Chinese context and has significant implications for teacher education.
Solid Lipid Nanoparticles and Nanostructured Lipid Carriers: Structure, Preparation and Application (2015)

Lipid nanoparticles (LNPs) have attracted special interest during last few decades. Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) are two major types of Lipid-based nanoparticles. SLNs were developed to overcome the limitations of other colloidal carriers, such as emulsions, liposomes and polymeric nanoparticles because they have advantages like good release profile and targeted drug delivery with excellent physical stability. In the next generation of the lipid nanoparticle, NLCs are modified SLNs which improve the stability and capacity loading. Three structural models of NLCs have been proposed. These LNPs have potential applications in drug delivery field, research, cosmetics, clinical medicine, etc. This article focuses on features, structure and innovation of LNPs and presents a wide discussion about preparation methods, advantages, disadvantages and applications of LNPs by focusing on SLNs and NLCs.
The conversion of citrate into cis-aconitate and isocitrate in the presence of aconitase (1952)

have expressed doubts on the inter- mediary formation of ci-aconitate in the aconitase system because a study of the kinetics of the con- version of citrate into isocitrate failed to show a lag period. The authors argue that if the conversion followed the original scheme of
Electrical Stimulation as Treatment for Obesity and Diabetes (2007)

The prevalence of obesity is growing, is driving an increase in the prevalence of diabetes, and is creating a major public health crisis in the United States. Lifestyle and behavior therapy rarely give durable weight loss. There are few medications approved for the treatment of obesity. Those that exist are limited in efficacy and using them in combination does not result in greater weight loss. Surgical treatments for obesity are effective and give durable weight loss, but are accompanied by measurable morbidity and mortality. Several pacing approaches are being tried and are an outgrowth of pacing for gastroparesis. The Transcend(R) pacemaker blocks vagal efferents and delays gastric emptying, giving a 40% loss of excess body weight, if certain screening procedures are employed. The Tantulus pacemaker is still in development but increases antral muscular contractions and delays gastric emptying by stimulation during the absolute refractory period. Weight loss has been 30% of excess body weight, and glycohemoglobin decreased 1.6% in a trial of obese type 2 diabetes. Stimulation to the subdiaphragmatic sympathetics, vagal nerve stimulation with or without unilateral vagotomy, and intestinal pacing are other approaches that are still being evaluated preclinically. Clearly a safe, effective, and durable treatment for obesity is desperately needed. Electrical pacing of the gastrointestinal tract is promising therapeutically, and because pacemakers work through different mechanisms, combining pacemaker treatments may be possible. Rapid progress is being made in the field of electrical stimulation as a treatment for obesity and even greater progress can be expected in the foreseeable future.
Agricultural Plants (1991)

The future of mankind depends on ever-increasing agricultural production to provide food, fibre, fuel and other essential commodities. This can only be achieved through a sound knowledge of the plants which feature prominently in agriculture. This book describes these plants in detail, together with the products which are obtained from them. The opening chapter on world population and food supply is followed by a general introduction to plant structure. The major part of the book then considers economically important field crops and pasture plants of temperate and subtropical regions on a family-by-family basis. Fruit crops, flowers and trees are not considered. The book concludes with a discussion of physiological principles of crop growth and yield. This is an introductory text, suitable for the teaching of agricultural botany to undergraduate students, and is intended to bridge the gap between classical botany and agronomy.
Single Step Reconstitution of Multifunctional High-Density Lipoprotein-Derived Nanomaterials Using Microfluidics (2013)

High-density lipoprotein (HDL) is a natural nanoparticle that transports peripheral cholesterol to the liver. Reconstituted high-density lipoprotein (rHDL) exhibits antiatherothrombotic properties and is being considered as a natural treatment for cardiovascular diseases. Furthermore, HDL nanoparticle platforms have been created for targeted delivery of therapeutic and diagnostic agents. The current methods for HDL reconstitution involve lengthy procedures that are challenging to scale up. A central need in the synthesis of rHDL, and multifunctional nanomaterials in general, is to establish large-scale production of reproducible and homogeneous batches in a simple and efficient fashion. Here, we present a large-scale microfluidics-based manufacturing method for single-step synthesis of HDL-mimicking nanomaterials (μHDL). μHDL is shown to have the same properties (e.g., size, morphology, bioactivity) as conventionally reconstituted HDL and native HDL. In addition, we were able to incorporate simvastatin (a hydrophobic drug) into μHDL, as well as gold, iron oxide, quantum dot nanocrystals or fluorophores to enable its detection by computed tomography (CT), magnetic resonance imaging (MRI), or fluorescence microscopy, respectively. Our approach may contribute to effective development and optimization of lipoprotein-based nanomaterials for medical imaging and drug delivery.
Mast cell IL-4 expression is regulated by Ikaros and influences encephalitogenic Th1 responses in EAE (2006)

When exposed to a pathogen, a naive CD4(+) T cell is forced to make a cell fate decision that leads to a polarized population of Th1 IFN-gamma- or Th2 IL-4- producing cells. Although IL-4 has traditionally been considered a factor that promotes Th2 cell differentiation, recent evidence has demonstrated that the site and timing of IL-4 expression in an immune response determines its ultimate effects on CD4(+) T cell fate. Using a mast cell (MC) reconstitution model, we demonstrate that MC-derived IL-4 promoted Th1 responses in vivo. Furthermore, MCs from genetically disparate mouse strains varied in their potential for IL-4 expression. Independent of the activation mode, MCs from Th1-prone C57BL/6 mice exhibited a more robust Il4 response than did the Th2-prone strain Balb/c. The hierarchy of IL-4 expression potential was directly associated with the degree of basal chromatin accessibility at cis-regulatory elements conserved noncoding sequence-1 and V(A) enhancer within the Th2 locus. GATA1/2 and Ikaros, factors with opposing roles in chromatin remodeling, acted at these sites. We propose that GATA and Ikaros proteins coordinately fine-tune accessibility at the Il4 locus during development to variably regulate IL-4 expression. These events likely contribute to the genetically determined heterogeneity in Th1 responses that underlie susceptibility to many diseases.
Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. (1987)

Synaptophysin is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. The authors have studied a wide spectrum of neuroendocrine (NE) neoplasms by immunofluorescence microscopy on cryostat sections of freshly frozen tissues using a monoclonal antibody to this protein (SY 38). Without exception, they found the identical--or a very similar--protein expressed in all neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, and paragangliomas studied. In these "neural" type NE neoplasms, synaptophysin was coexpressed with neurofilament proteins. Synaptophysin was also demonstrated in NE neoplasms of "epithelial" type in which it was predominantly coexpressed with cytokeratins and desmoplakin. It was invariably found in all variants of islet cell neoplasms and in all medullary thyroid carcinomas. Synaptophysin was also demonstrated in several adenomas of the hypophysis and parathyroids, in the majority of carcinoids of the bronchopulmonary and gastrointestinal tracts, and in many, though not all, NE carcinomas of the same sites, and of the skin. Conversely, SY 38 did not immunostain any of a large number of benign and malignant non-NE epithelial neoplasms; nor was any immunostaining obtained in a group of mesenchymal tumors. It is remarkable that SY 38 did not immunostain a number of malignant melanomas, including several that were immunostained for neuron-specific enolase (NSE) and several neuropeptides. Parallel studies conducted on conventionally fixed, paraffin-embedded tissue sections immunostained by the use of the avidin-biotin complex technique yielded very similar results. The findings indicate that synaptophysin is expressed in the whole range of NE neoplasms without detectable relation to the expression of other NE markers such as NSE, serotonin, and neuropeptides. Nor could the expression of synaptophysin by these tumors be correlated with their epithelial and/or neural cytoskeletal characteristics, their clinical aggressiveness, or the presence or absence of endocrinologic abnormalities. While the consistent expression of synaptophysin by the "neural" type of NE neoplasms would seem predictable its presence in diverse benign and malignant NE tumors of "epithelial" type is remarkable. It is concluded that synaptophysin is a significant as well as novel NE marker, and the use of antibody SY 38 as a broad range marker for the study and diagnosis of NE neoplasms is proposed.
Detection of Distribution High Impedance Faults Using Burst Noise Signals near 60 HZ (1987)

Previous papers have described a method for the detection of arcing fallen distribution primiary conductor faults using the electrical noise in feeder current above 2kHz. While this method provided improved detection of such faults, this high frequency signal often would not propagate past capacitor banks. In the present paper, we describe a technique for the identification of arcing high impedance faults using burst noise signals at frequencies near the power system fundamental and low order harmonics. Arcing generates non-synchronous burst noise signals which approximate white noise, providing a signal which can be differentiated from synchronous power system signals in the frequency bands of interest. The primary advantage of monitoring frequencies near the fundamental is that this arcing fault signal at low frequencies will exhibit little attenuation from capacitor banks or other sources. This paper provides preliminary results that arcing faults can be detected effectively using frequency components below 60 Hz or between low order harmonics of 60 Hz. The technique is demonstrated through analysis of analog signals recorded during numerouis staged utility downed conductor tests.
Analysis of the Short Consensus Repeats of Human Complement Factor B by Site-directed Mutagenesis (1995)

Human factor B is required for the initiation and propagation of the complement alternative pathway. It also participates in the amplification of the complement classical pathway. Alone, factor B is a zymogen with little known biochemical activity, but in the context of the alternative pathway convertases, the factor B serine protease is activated in a process that first involves the association with C3b and subsequently the cleavage of factor B into two fragments, Ba and Bb. Ba, the NH2-terminal fragment, is composed mainly of three tandem short consensus repeats, globular domains found in other complement proteins. It dissociates from the convertase during assembly, leaving the active C3 convertase, C3bBb. Previous reports suggest that the Ba region may be instrumental in convertase assembly. This hypothesis was tested using site-directed mutagenesis of recombinant factor B and monoclonal antibody epitope mapping to evaluate the relative importance of specific short consensus repeat amino acid residues. Three sites of interest were identified. Site 1 is a stretch of 19 contiguous amino acids in short consensus repeat 1 that form the epitope of a monoclonal antibody that effectively blocks factor B function. Site 2, composed of 6 contiguous amino acids in short consensus repeat 2, and site 3, consisting of 7 contiguous amino acids in short consensus repeat 3, were defined by mutations that reduce factor B hemolytic activity to 3% or less. Further analyses indicated that sites 2 and 3 contribute to factor B-C3b interactions.
Inhibition of chick embryo lysyl oxidase by various lathyrogens and the antagonistic effect of pyridoxal. (1992)

Lysyl oxidase, which cross-links collagen and elastin, was obtained from chick embryo bone and cartilage and its substrate, elastin, from aorta. The enzyme was studied using an improved assay which enabled the stability of the substrate to be monitored. The enzyme was fully inhibited in vivo by beta-aminopropionitrile, semicarbazide, thiosemicarbazide and isoniazid and in vitro by beta-aminopropionitrile and semicarbazide but only partially by thiosemicarbazide and isoniazid. Penicillamine, which solubilizes collagen by labilizing Schiff base cross-links in vivo and which prevents stable cross-link formation in vitro indirectly by binding to aldehyde groups on collagen, was shown to have no direct inhibitory effect on lysyl oxidase in vivo or in vitro. Homocysteine, which also solubilizes collagen by a mechanism similar to penicillamine does not inhibit lysyl oxidase either in vivo or in vitro. Pyridoxal reversed the inhibition of lysyl oxidase by semicarbazide and isoniazid in vivo but was unable to reverse that produced by either beta-aminopropionitrile or thiosemicarbazide. These results can be explained by the presence of a sulphydryl group near the active site of lysyl oxidase, which can form a complex with the nitrile group on beta-aminopropionitrile or with the thiol group on thiosemicarbazide leading to irreversible inhibition.
B-RAF Regulation of Rnd3 Participates in Actin Cytoskeletal and Focal Adhesion Organization (2007)

The actin cytoskeleton controls multiple cellular functions, including cell morphology, movement, and growth. Accumulating evidence indicates that oncogenic activation of the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase 1/2 (MEK/ERK1/2) pathway is accompanied by actin cytoskeletal reorganization. However, the signaling events contributing to actin cytoskeleton remodeling mediated by aberrant ERK1/2 activation are largely unknown. Mutant B-RAF is found in a variety of cancers, including melanoma, and it enhances activation of the MEK/ERK1/2 pathway. We show that targeted knockdown of B-RAF with small interfering RNA or pharmacological inhibition of MEK increased actin stress fiber formation and stabilized focal adhesion dynamics in human melanoma cells. These effects were due to stimulation of the Rho/Rho kinase (ROCK)/LIM kinase-2 signaling pathway, cumulating in the inactivation of the actin depolymerizing/severing protein cofilin. The expression of Rnd3, a Rho antagonist, was attenuated after B-RAF knockdown or MEK inhibition, but it was enhanced in melanocytes expressing active B-RAF. Constitutive expression of Rnd3 suppressed the actin cytoskeletal and focal adhesion effects mediated by B-RAF knockdown. Depletion of Rnd3 elevated cofilin phosphorylation and stress fiber formation and reduced cell invasion. Together, our results identify Rnd3 as a regulator of cross talk between the RAF/MEK/ERK and Rho/ROCK signaling pathways, and a key contributor to oncogene-mediated reorganization of the actin cytoskeleton and focal adhesions.
Determining calcium‐binding stoichiometry and cooperativity of parvalbumin and calmodulin by mass spectrometry (1995)

Abstract The calcium‐binding properties of parvalbumin and calmodulin were investigated by electrospray ionization mass spectrometry (ESI‐MS). The two calcium sites of parvalbumin were found to be strongly cooperative in binding Ca 2+ ion. Up to four calcium ions were found to bind to calmodulin at high calcium concentration levels. Strong cooperativity was detected between the third and fourth Ca 2+ binding sites of calmodulin (ordered by loading sequence). Strong interactions were also indicated between the two halves of the calmodulin molecule. Demetallation of the gas‐phase ions can occur during the desolvation process, especially for ESI of aqueous solutions. However, the ESI‐MS methodology has the potential to provide insight into the intricate processes involved in metal site communications for other metalloproteins.
2002 Global update of available data on visual impairment: a compilation of population-based prevalence studies (2004)

Two-hundred-and-eight population-based studies on visual impairment for 68 countries are reported in detail, providing an up-to-date, comprehensive compilation of the available information on visual impairment and its causes globally.
A Study of Highly Oriented Pyrolytic Graphite as a Model for the Graphite Anode in Li‐Ion Batteries (1999)

The mechanisms of oxidation of the basal plane and of the cross‐sectional face of highly oriented pyrolytic graphite (HOPG) and the formation of a solid electrolyte interphase (SEI) on HOPG samples that were cycled in ethylene carbonate:diethyl carbonate (EC:DEC 1:2) solutions containing 1 M were studied. X‐ray photoelectron spectroscopy, energy dispersive spectrometry, and scanning electron microscope techniques were used for the analysis of the surface layer formed on the basal plane and cross section of HOPG. The analysis indicates that the oxidation mechanisms of the basal plane and the cross section are entirely different. The SEI formed in the solution is thinner on the basal plane than on the cross section and its composition is different. The SEI formed on the cross section is rich in inorganic compounds whereas the SEI formed on the basal plane is rich in organic compounds. Thus it can be concluded that on the basal plane, the greatest contribution to SEI formation is solvent reduction (EC and DEC), whereas on the cross‐sectional face, it is electrolyte salt reduction. © 1999 The Electrochemical Society. All rights reserved.
Disease-modifying therapies for Alzheimer disease (2007)

Prevention of Alzheimer disease (AD) is a national and global imperative. Therapy is optimally initiated when individuals are asymptomatic or exhibit mild cognitive impairment (MCI). Development of therapeutically beneficial compounds requires the creation of clinical trial methodologies for primary and secondary prevention. Populations in primary prevention trials selected only on the basis of age will have low rates of emergent MCI or AD. Epidemiologically based risk factors or biomarkers can be used to enrich trials and increase the likelihood of disease occurrence during the trial. Enrichment strategies for clinical trials with MCI include use of biomarkers such as amyloid imaging, MRI with demonstration of medial temporal lobe atrophy, bilateral parietal hypometabolism on PET, and reduced amyloid beta peptide and increased tau protein in CSF. Neuropsychological measures appropriate for trials of MCI may not be identical to those measures most suited for AD trials. Attention to these and other features of trial design, clinical assessment, and use of biomarkers is critical to improving the detection of disease-modifying effects of emerging therapies in presymptomatic or minimally symptomatic populations. The neurologic health of the growing aging population demands disease-modifying therapies and the development of methods to identify and test promising candidate agents.
The status of Bogidiella Balearica Dancau, 1973, a stygobiont amphipod from Mallorca (1987)

&amp;#091;spa&amp;#093; Se presenta la redescripción de Bogidiella balearica Dancau, 1973, un anfípodo estigobionte de las cuevas anquihalinas de Mallorca. Queda demostrado que se trata de una buena especie, clasificable dentro del subgénero Bogidiella s. str., y que se puede diferenciar fácilmente de Bogidiella chappuisi Ruffo, 1952 (con la cual se intentó sinonimizar con anterioridad), perteneciente a otro subgénero, Medigidiella.
Nuclear Delivery of Antisense Oligodeoxynucleotides Through Reversible Permeabilization of Human Leukemia Cells with Streptolysin O (1995)

Most mammalian cell types appear to take up antisense oligonucleotides and oligonucleotide analogs from the bathing medium by highly inefficient endocytic mechanisms, and most if not all intracellular oligomer is sequestered in vesicles, still separated by a membrane from the target mRNA. On the other hand, oligonucleotides introduced directly into the cytoplasm by microinjection rapidly accumulate in the cell nucleus. Poor delivery to the designated site of action of antisense oligonucleotides is a major problem limiting their routine use in genetic research and their development as potential therapeutic agents. In view of this difficulty, various means of membrane permeabilization were applied to cultured human leukemia cells in an attempt to enhance intracytoplasmic delivery of fluorescein-tagged oligodeoxynucleotides. The outcome of the manipulations was monitored by flow cytometry and fluorescence microscopy. This work has directly confirmed the conclusion suggested by reported antisense effects, that streptolysin O reversibly permeabilizes the plasma membrane toward oligonucleotides and may be utilized to effect biochemical "microinjection" of these molecules directly into the cytoplasm. KY01 myelogenous leukemia cells treated in this way accumulated over 100-fold higher intracellular levels of oligodeoxynucleotides than in the absence of streptolysin O and, in contrast to the latter case, were observed to concentrate internalized molecules in their nuclei.