gebhart/patrepeval huggingface dataset:
Cholesterol depletion disrupts lipid rafts and modulates the activity of multiple signaling pathways in T lymphocytes (2000)

Lipid rafts are specialized plasma membrane microdomains, in which glycosphingolipids and cholesterol are major structural components. In T lymphocytes, several signaling proteins are associated with lipid rafts including the protein tyrosine kinase LCK and the adapter protein LAT. To investigate their importance in T cell signaling, lipid rafts were disrupted by depleting cholesterol with methyl-beta-cyclodextrin (MbetaCD). This transiently induced tyrosine phosphorylation of multiple proteins, including the ZAP-70 tyrosine kinase, its associated T cell antigen receptor zeta chain, LAT and phospholipase Cgamma1. Tyrosine phosphorylation was dependent on expression of LCK in lipid rafts. Depletion of cholesterol also resulted in activation of the Ras-ERK pathway. This was largely dependent on phorbol ester-sensitive protein kinase C (PKC) and the PKC-theta isoform translocated to the plasma membrane following MbetaCD treatment. MbetaCD did not stimulate intracellular Ca2+ fluxes; however, consistent with its ability to stimulate Ras, MbetaCD synergized with a Ca2+ ionophore to induce formation of the transcription factor NF-AT. These data indicate a crucial role for cholesterol in the regulation of signaling pathways in T cells, which is likely to reflect its importance in the formation of plasma membrane lipid rafts.
A Novel Approach for Detecting Browser-Based Silent Miner (2018)

Transactions in the cryptocurrency market has been extremely hot in recent years, with the price of cryptocurrency climbing all the way. Hackers have turned their attentions to cryptocurrencies, and have used various means to acquire cryptocurrencies illegally, which caused huge losses to the victims. Some browsers block malicious mining activities from the network protocol level, but they do not have the ability to detect mining samples themselves, and it is difficult to make effective detection of homogenous mining samples of the network layer. To solve these problems, based on the attack pattern of browser mining, the browser-based silent mining features are analyzed, and a method to detect browser silent mining behavior is proposed. This method drives known malicious mining samples, extracts heap snapshots and stack code features of a dynamically running browser, and performs automated detection based on recurrent neural network. By modifying the kernel code of Chrome, a browser-based silent miner detection prototype system BMDetector was designed and implemented. With 1159 samples detected and analyzed, experimental results show that the recognition rate of the original mining sample is 98%, and 92% for the encrypted and confused, which is an effective and feasible method.
Enhanced nitrogen removal in SBRs bypassing nitrate generation accomplished by multiple aerobic/anoxic phase pairs (2003)

A lab-scale SBR was used for the study of nitrogen removal from a synthetic wastewater with an ammonium-nitrogen concentration of 50 mg/L. The react phase of the reactor operation was divided into three sets of consecutive aerobic and anoxic periods with a duration ratio of 1:3 (20 min aerobic and 1 h anoxic phase). Under these operating conditions, nitrogen removal was achieved via nitrite i.e. no nitratification (oxidation of nitrite to nitrate) and hence no denitratification (reduction of nitrate to nitrite) was taking place in the aerobic and anoxic phase, respectively. This was attributed to the suppression of the nitrite-oxidizers activity due to the short aerobic phase duration. This presumption was supported by the ever decreasing amount of nitrate-nitrogen generated in the react phase during the transient, even when the activated sludge of the reactor was supplemented with additional nitrite-oxidizers. On the other hand, denitrification was mainly based on stored carbon sources, as long as the organic carbon (provided in the form of acetate) was never accumulated during the anoxic/anaerobic fill phase of the reactor operation.
α‐Caryopteron, ein neues Pyrano‐juglon aus Caryopteris clandonensis (1969)

Abstract Caryopteris clandonsis B UNGE ( Verbenaceae ) enthält in der Wurzelrinde mehrere Farbstoffe, von denen einer, α‐Caryopteron, C 15 H 12 O 4 , kristallisiert und rein erhalten werden konnte. Er wurde als 9‐Hydroxy‐α‐dehydrolapachon (Ia) erkannt, wobei die Lage der Hydroxylgruppe unter Beiziehung der para ‐ ortho ‐Chinonmlagerung nach P ATERNÒ ;‐H OOKER , am Dihydroderivat festgelegt wurde.
The Cross‐Functional Imperative: The Case of Marketing and Purchasing (1994)

As purchasers increasingly seek ways to enhance performance in interfunctional work teams, it is essential to understand how and why various functions interact in a given manner. This article underscores how marketing and purchasing can learn from each other to heighten effectiveness and goal attainment. In particular, specific areas in which the two functions share commonalities are highlighted.
Comparative studies of the inhibitory properties of antibiotics on human immunodeficiency virus and avian myeloblastosis virus reverse transcriptases and cellular DNA polymerases. (1989)

The inhibition of human immunodeficiency virus (HIV) reverse transcriptase by certain antibiotics and related compounds was studied in comparison with that of avian myeloblastosis virus (AMV) reverse transcriptase and cellular DNA polymerases alpha and beta. In general, compounds that inhibited HIV reverse transcriptase also inhibited AMV reverse transcriptase. For example, 10 micrograms/ml of the isoquinoline quinones used in this study inhibited approximately 80% of the activity of reverse transcriptases of HIV and AMV, but did not inhibit the activity of DNA polymerases alpha and beta even at 50 micrograms/ml. AMV enzyme was more sensitive than HIV enzyme to colistin, enduracidins A and B, janiemycin, glysperin A, and thielavins A and B. The streptonigrin alkyl esters, however, inhibited HIV reverse transcriptase only. Sakyomicin A, luzopeptins, ellagic acid and suramine inhibited the activities of reverse transcriptases and cellular DNA polymerases.
Organization of the murine Mx gene and characterization of its interferon- and virus-inducible promoter. (1988)

Specific resistance of Mx+ mice to influenza virus is due to the interferon (IFN)-induced protein Mx. The Mx gene consists of 14 exons that are spread over at least 55 kilobase pairs of DNA. Surprisingly, the Mx gene promoter is induced as efficiently by Newcastle disease virus as it is by IFN. The 5' boundary of the region required for maximal induction by both IFN and Newcastle disease virus is located about 140 base pairs upstream of the cap site. This region contains five elements of the type GAAANN, which occurs in all IFN- and virus-inducible promoters. The consensus sequence purine-GAAAN(N/-)GAAA(C/G)-pyrimidine is found in all IFN-inducible promoters.
Inter-Vehicle Range Estimation from Periodic Broadcasts (2015)

Dedicated short-range communication (DSRC) enables vehicular communication using periodic broadcast messages. We propose to use these periodic broadcasts to perform inter-vehicle ranging. Motivated by this scenario, we study the general problem of precise range estimation between pairs of moving vehicles using periodic broadcasts. Each vehicle has its own independent and unsynchronized clock, which can exhibit significant drift between consecutive periodic broadcast transmissions. As a consequence, both the clock offsets and drifts need to be taken into account in addition to the vehicle motion to accurately estimate the vehicle ranges. We develop a range estimation algorithm using local polynomial smoothing of the vehicle motion. The proposed algorithm can be applied to networks with arbitrary number of vehicles and requires no additional message exchanges apart from the periodic broadcasts. We validate our algorithm on experimental data and show that the performance of the proposed approach is close to that obtained using unicast round-trip time ranging. In particular, we are able to achieve sub-meter ranging accuracies in vehicular scenarios. Our scheme requires additional timestamp information to be transmitted as part of the broadcast messages, and we develop a novel timestamp compression algorithm to minimize the resulting overhead.
Readmission and Late Mortality After Pediatric Severe Sepsis (2009)

Late death occurred with similar frequency as early death associated with hospitalization with severe sepsis. Almost half of the pediatric patients suffering from an episode of severe sepsis had at least 1 subsequent hospitalization, two thirds of which were emergent or urgent. These data suggest that late outcomes after an episode of severe sepsis are poor and call for the evaluation of interventions designed to reduce later morbidity and mortality.
Quality of life in breast cancer. (1997)

The data demonstrated the need for further research, assessment, and intervention across each of the quality-of-life domains. There is a significant need to address physical problems; however, the psychological domain demonstrated the greatest area of distress. The multidimensional needs of breast cancer survivors emphasize the need for multidisciplinary collaboration.
Targeting IL-15 Receptor-Bearing Cells with an Antagonist Mutant IL-15/Fc Protein Prevents Disease Development and Progression in Murine Collagen-Induced Arthritis (2004)

It has been suggested that the inflammatory cytokine IL-15 plays an important role in the development of several autoimmune diseases, including rheumatoid arthritis. We have generated a unique lytic and antagonistic IL-15 mutant/Fcgamma2a fusion protein (CRB-15) that targets the IL-15R. In the present study we examined the effects of targeting the IL-15R on the prevention and treatment of collagen-induced arthritis (CIA) in mice and probed the possible mechanisms of action of this IL-15 mutant/Fcgamma2a protein. Upon immunization with type II collagen, DBA/1 mice develop severe articular inflammation and destruction. Treatment of DBA/1 mice with a brief course of CRB-15 at the time of type II collagen challenge markedly inhibited the incidence and severity of arthritis. Moreover, in animals with ongoing established arthritis, treatment with CRB-15 effectively blocked disease progression compared with that in control-treated animals. The therapeutic effect of CRB-15 on either disease development or disease progression is remarkably stable, because withdrawal of treatment did not lead to disease relapse. A detailed analysis revealed that treatment with CRB-15 decreased synovitis in the joints; reduced bone erosion and cartilage destruction; reduced in situ production of the proinflammatory cytokines TNF-alpha, IL-1beta, IL-6, and IL-17; and decreased the responder frequency of autoreactive T cells. Our study suggests that the effective targeting of IL-15R-triggered events with CRB-15 can be of therapeutic importance in the treatment of rheumatoid arthritis.
Glycylcyclines. 1. A new generation of potent antibacterial agents through modification of 9-aminotetracyclines (1994)

This report describes the discovery of a new generation of tetracycline antibacterial agents, the "glycylcyclines". These agents are notable for their activity against a broad spectrum of tetracycline-susceptible and -resistant Gram-negative and Gram-positive aerobic and anaerobic bacteria possessing various classes of tetracycline-resistant determinants [tet B (efflux), tet M (ribosomal protection)]. The design and synthesis of a number of 7-substituted 9-substituted-amido 6-demethyl-6-deoxytetracyclines are described.
Combining Insulin-Like Growth Factor Derivatives Plus Caffeinol Produces Robust Neuroprotection After Stroke in Rats (2004)

Both GPE and caffeinol significantly protect cortex after MCA occlusion. At the doses used in this study, the GPE analogue G2MePE by itself had minimal protective effects, but when combined with caffeinol, it demonstrated robust beneficial effects on cortical and subcortical lesion size and behavioral deficit. Further study of this combination appears justified.
Immune Surveillance Properties of Human NK Cell-Derived Exosomes (2012)

Exosomes are nanovesicles released by normal and tumor cells, which are detectable in cell culture supernatant and human biological fluids, such as plasma. Functions of exosomes released by "normal" cells are not well understood. In fact, several studies have been carried out on exosomes derived from hematopoietic cells, but very little is known about NK cell exosomes, despite the importance of these cells in innate and adaptive immunity. In this paper, we report that resting and activated NK cells, freshly isolated from blood of healthy donors, release exosomes expressing typical protein markers of NK cells and containing killer proteins (i.e., Fas ligand and perforin molecules). These nanovesicles display cytotoxic activity against several tumor cell lines and activated, but not resting, immune cells. We also show that NK-derived exosomes undergo uptake by tumor target cells but not by resting PBMC. Exosomes purified from plasma of healthy donors express NK cell markers, including CD56+ and perforin, and exert cytotoxic activity against different human tumor target cells and activated immune cells as well. The results of this study propose an important role of NK cell-derived exosomes in immune surveillance and homeostasis. Moreover, this study supports the use of exosomes as an almost perfect example of biomimetic nanovesicles possibly useful in future therapeutic approaches against various diseases, including tumors.
Apoptotic cell-based therapies against transplant rejection: role of recipient’s dendritic cells (2010)

One of the ultimate goals in transplantation is to develop novel therapeutic methods for induction of donor-specific tolerance to reduce the side effects caused by the generalized immunosuppression associated to the currently used pharmacologic regimens. Interaction or phagocytosis of cells in early apoptosis exerts potent anti-inflammatory and immunosuppressive effects on antigen (Ag)-presenting cells (APC) like dendritic cells (DC) and macrophages. This observation led to the idea that apoptotic cell-based therapies could be employed to deliver donor-Ag in combination with regulatory signals to recipient's APC as therapeutic approach to restrain the anti-donor response. This review describes the multiple mechanisms by which apoptotic cells down-modulate the immuno-stimulatory and pro-inflammatory functions of DC and macrophages, and the role of the interaction between apoptotic cells and APC in self-tolerance and in apoptotic cell-based therapies to prevent/treat allograft rejection and graft-versus-host disease in murine experimental systems and in humans. It also explores the role that in vivo-generated apoptotic cells could have in the beneficial effects of extracorporeal photopheresis, donor-specific transfusion, and tolerogenic DC-based therapies in transplantation.
Guidance of neurite outgrowth by pathways of substratum‐adsorbed laminin (1985)

Neurite outgrowth is guided by narrow pathways of bioactive laminin. These pathways are created by ultraviolet light irradiation of laminin-coated coverslips masked with electron microscope grids. Patterned outgrowth of neurites is independent of gross mechanical guidance and guidance caused by substrate limitation. Cells on unirradiated laminin are less readily displaced by shear forces than cells on irradiated laminin. This study suggests that ultraviolet light alters the adhesive properties of laminin and that differential cell-substratum adhesion may guide extending neurites on the purified naturally occurring substance, laminin.
A Single Immunization with Soluble Recombinant Trimeric Hemagglutinin Protects Chickens against Highly Pathogenic Avian Influenza Virus H5N1 (2010)

Our results demonstrate that sH5(3) constitutes an attractive vaccine antigen for protection of chickens and mammals against HPAI H5N1. As these recombinant soluble hemagglutinin preparations can be produced with high yields and with relatively short lead time, they enable a rapid response to circulating and potentially pandemic influenza viruses.
Heuristic Method for Phase-Conflict Minimization in Automatic Phase-Shift Mask Design (1995)

A shifter placement program has been developed for alternate-type phase-shift masks that require a 180°-phase difference between adjacent aperture pairs. Using a heuristic approach, this program minimizes the number of phase conflicts that arise when adjacent apertures are forced to have the same phase. Adjacent aperture relationships are expressed by an undirected graph; the phase conflicts are found in the closed loops containing an odd number of apertures. Minimization is achieved by listing these loops and identifying the adjacent aperture pairs that occur in more than one loop. The validity of this algorithm was demonstrated using test patterns.
Implications of the three-dimensional structure of .alpha.1-antitrypsin for structure and function of serpins (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImplications of the three-dimensional structure of .alpha.1-antitrypsin for structure and function of serpinsR. Huber and R. W. CarrellCite this: Biochemistry 1989, 28, 23, 8951–8966Publication Date (Print):November 14, 1989Publication History Published online1 May 2002Published inissue 14 November 1989https://pubs.acs.org/doi/10.1021/bi00449a001https://doi.org/10.1021/bi00449a001research-articleACS PublicationsRequest reuse permissionsArticle Views1320Altmetric-Citations719LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Inhibition of MerTK increases chemosensitivity and decreases oncogenic potential in T-cell acute lymphoblastic leukemia (2013)

Pediatric leukemia survival rates have improved dramatically over the past decades. However, current treatment protocols are still largely ineffective in cases of relapsed leukemia and are associated with a significant rate of chronic health conditions. Thus, there is a continued need for new therapeutic options. Here, we show that mer receptor tyrosine kinase (MerTK) was abnormally expressed in approximately one half of pediatric T-cell leukemia patient samples and T-cell acute lymphoblastic leukemia (T-ALL) cell lines. Stimulation of MerTK by the ligand Gas6 led to activation of the prosurvival proteins Erk 1/2 and Stat5, and MerTK-dependent activation of the STAT pathway in leukemia represents a novel finding. Furthermore, inhibition of MerTK expression increased the sensitivity of T-ALL cells to treatment with chemotherapeutic agents and decreased the oncogenic potential of the Jurkat T-ALL cell line in a methylcellulose colony-forming assay. Lastly, inhibition of MerTK expression significantly increased median survival in a xenograft mouse model of leukemia (30.5 days vs 60 days, P<0.0001). These results suggest that inhibition of MerTK is a promising therapeutic strategy for the treatment of leukemia and may allow for dose reduction of currently used chemotherapeutics resulting in decreased rates of therapy-associated toxicities.
Early Restriction of the Human Antibody Repertoire (1987)

Diversification of the antibody repertoire in mammals results from a series of apparently random somatically propagated gene rearrangement and mutational events. Nevertheless, it is well known that the adult repertoire of antibody specificities is acquired in a developmentally programmed fashion. As previously shown, rearrangement of the gene segments encoding the heavy-chain variable regions (VH) of mouse antibodies is also developmentally ordered: the number of VH gene segments rearranged in B lymphocytes of fetal mice is small but increased progressively after birth. In this report, human fetal B-lineage cells were also shown to rearrange a highly restricted set of VH gene segments. In a sample of heavy-chain transcripts from a 130-day human fetus the most frequently expressed human VH element proved to be closely related to the VH element most frequently expressed in murine fetal B-lineage cells. These observations are important in understanding the development of immunocompetence.
Decentralized Filtering and Redundancy Management for Multisensor Navigation (1987)

Failure detection and redundancy management is discussed for avionics applications of integrated navigation involving coordinated use of multiple simultaneous sensor subsystems such as GPS, JTIDS, TACAN, VOR/DME, ILS, an inertial navigation system (INS), and possibly even Doppler AHRS. A brief high level survey is provided to assess the status of those techniques and methodologies advertized as already available for handling the challenging real-time failure detection, redundancy management, and Kalman filtering aspects of these systems with differing availabilities, differing reliabilities, differing accuracies, and differing information content/sampling rates. Following the status review, a new failure detection/redundancy management approach is developed based on voter/monitoring at both the raw data and at the filtered-data level, as well as using additional inputs from hardware built-in-testing (BIT) and from specialized tests for subsequent failure isolation in the case of ambiguous indications. The technique developed involves use of Gaussian confidence regions to reasonably account for the inherent differences in accuracy between the various sensor subsystems. Online estimates of covariances from the Kalman filter are to be used for this purpose (when available). A technique is provided for quantitatively evaluating both the probability of detecting failed component subsystems and the probability of false alarm to be incurred, which is then to be traded off as the basis for rational selection of the thresholds used in the automated decision process. Moreover, the redundancy management procedure is demonstrated to be amenable to pilot or navigation operator prompting and override, if necessary.
Total Synthesis of Siastatin B and Its Enantiomer Using Carbohydrate as a Chiral Educt (1992)

Abstract The enantioselective synthesis of siastatin B, an inhibitor of neuraminidase and its antipode from d-ribono-γ-lactone has stereospecifically been achieved. The total synthesis elucidated the absolute configuration of siastatin B as (3S,4S,5R,6R)-6-acetamido-4,5-dihydroxy-3-piperidinecarboxylic acid.
Protease-Inhibitoren im menschlichen Spermaplasma (1971)

Protease inhibitors in human sperm plasma.Isolation by affinity chromatography and inhibition characteristics.For the isolation of protease inhibitors from human sperm plasma the following procedures were used: adsorption on CM-Sephadex, affinity chromatography with trypsin cellulose-resin and gel filtration on Sephadex G-75.Two inhibitors wereIn den mnnlichen accessorischen Geschlechtsdrsen und im Sperma