gebhart/patrepeval huggingface dataset:
Design of a wireless backhaul network for microcells (2003)

The use of microcells is an attractive option for providing high data rate access for fixed and low mobility users. We propose and analyze a wireless backhaul network for connecting microcells to the wireline network. In the proposed network, several microcellular radio ports are connected to a so called hub through radio links. These hubs are then connected to the wireline network using cable or fiber. For ease of deployment, we propose that omnidirectional antennas be used at the radio ports and sectored antennas be used at the hubs. Our results show that for 95% of the links, a link reliability of 99% may be achieved with an effective frequency reuse of 3 by using site-selection, flexible channel assignment, power control and two-branch minimum mean square error combining. For the remaining 5% of the more vulnerable links, a direct wireline connection may be provided. We also find that power control plays a critical role in reducing the variation of the link performance over time. In addition, the best performance is achieved by jointly optimizing the receiver, i.e., the diversity combining weights, and the transmit power settings. We show that this joint optimization may be implemented in a distributed manner, making it attractive for use in real systems.
Image placement issues for ITO-based step and flash imprint lithography templates (2004)

Step and flash imprint lithography (SFIL) is an attractive, low-cost method for printing sub-100 nm geometries. The imprint process is performed at low pressures and room temperature, which minimizes magnification and distortion errors. Since SFIL is a 1× lithography technology, the template will require precise image placement in order to meet overlay specifications for multiple level device fabrication. In order to simplify the template fabrication process and facilitate post fabrication scanning-electron-microscope-based inspection, an integrated charge dissipation layer, such as indium tin oxide (ITO), is desired that is transparent to the SFIL exposure wavelength. The use of low-stress dielectric films such as SiON for the image relief layer minimizes the pattern distortions (&amp;lt;9 nm, mean+3σ) that occur after the pattern transfer process. Although ITO uniformity was also significantly improved by switching the ITO deposition process to an MRC sputter deposition system, image placement results were adversely affected.
From Peptide to Protein:  Comparative Analysis of the Substrate Specificity of N-Linked Glycosylation in C. jejuni (2007)

The gram-negative bacterium Campylobacter jejuni was recently discovered to contain a general N-linked protein glycosylation pathway. Central to this pathway is PglB, a homologue of the Stt3p subunit of the eukaryotic oligosaccharyl transferase (OT), which is involved in the transfer of an oligosaccharide from a polyisoprenyl pyrophosphate carrier to the asparagine side chain of proteins within the conserved glycosylation sites D/E-X1-N-X2-S/T, where X1 and X2 can be any amino acids except proline. Using a library of peptide substrates and a quantitative radioactivity-based in vitro assay, we assessed the amino acids at each position of the consensus glycosylation sequence for their impact on glycosylation efficiency, whereby the sequence DQNAT was found to be the optimal acceptor substrate. In the context of a full-length folded protein, the differences between variations of the glycosylation sequences were found to be consistent with the trends observed from their peptidyl counterparts, though less dramatic because of additional influences. In addition to characterizing the acceptor preferences of PglB, we also assessed the selectivity toward the glycan donor. Interestingly, despite recent reports of relaxed selectivity toward the glycan donor, PglB was not found to be capable of utilizing glycosyl donors such as dolichyl-pyrophosphate-chitobiose, which is the minimum substrate for the eukaryotic OT process.
Shrinker: efficient live migration of virtual clusters over wide area networks (2012)

SUMMARY Live virtual machine migration is a powerful feature of virtualization technologies. It enables efficient load balancing, reduces energy consumption through dynamic consolidation, and makes infrastructure maintenance transparent to users. Although live migration is available across wide area networks with state of the art systems, it remains expensive to use because of the large amounts of data to transfer, especially when migrating virtual clusters rather than single virtual machine instances. As evidenced by previous research, virtual machines running identical or similar operating systems have significant portions of their memory and storage containing identical data. We propose Shrinker, a live virtual machine migration system leveraging this common data to improve live virtual cluster migration between data centers interconnected by wide area networks. Shrinker detects memory pages and disk blocks duplicated in a virtual cluster to avoid sending the same content multiple times over wide‐area network links. Virtual machine data is retrieved in the destination site with distributed content‐based addressing. We implemented a prototype of Shrinker in the KVM (Kernel‐based Virtual Machine) hypervisor and present a performance evaluation in a distributed environment. Experiments show that it reduces both total data transferred and total migration time. Copyright © 2012 John Wiley &amp; Sons, Ltd.
Chylomicron metabolism (1989)

Previously it was shown in rabbits that 20-40% of the injected dose of chylomicrons was cleared from the plasma by perisinusoidal bone marrow macrophages. The present study was undertaken to determine whether the bone marrow of other species also cleared significant amounts of chylomicrons. Canine chylomicrons, labeled in vivo with [14C]cholesterol and [3H] retinol, were injected into marmosets (a small, New World primate), rats, guinea pigs, and dogs. Plasma clearance and tissue uptake of chylomicrons in these species were contrasted with results obtained in rabbits in parallel studies. The chylomicrons were cleared rapidly from the plasma in all animals; the plasma clearance of chylomicrons was faster in rats, guinea pigs, and dogs compared with their clearance from the plasma of rabbits and marmosets. The liver was a major site responsible for the uptake of these lipoproteins in all species. However, as in rabbits, the bone marrow of marmosets accounted for significant levels of chylomicron uptake. The uptake by the marmoset bone marrow ranged from one-fifth to one-half the levels seen in the liver. The marmoset bone marrow also took up chylomicron remnants. Perisinusoidal macrophages protruding through the endothelial cells into the marrow sinuses were responsible for the accumulation of the chylomicrons in the marmoset bone marrow, as determined by electron microscopy. In contrast to marmosets, chylomicron clearance by the bone marrow of rats, guinea pigs, and dogs was much less, and the spleen in rats and guinea pigs took up a large fraction of chylomicrons. The uptake of chylomicrons by the non-human primate (the marmoset), in association with the observation that triglyceride-rich lipoproteins accumulate in bone marrow macrophages in patients with type I, III, or V hyperlipoproteinemia, suggests that in humans the bone marrow may clear chylomicrons from the circulation. It is reasonable to speculate that chylomicrons have a role in the delivery of lipids to the bone marrow as a source of energy and for membrane biosynthesis or in the delivery of fat-soluble vitamins.
Lipid Tubules: A Paradigm for Molecularly Engineered Structures (1993)

The use of molecular self-assembly to fabricate microstructures suitable for advanced material development is described. Templating techniques that transform biomolecular self-assemblies into rugged and stable nano- and microstructures are described. By using a lipid-based microcylinder (tubule) as a paradigm, the path followed from research and development to emerging technological applications is detailed. This process includes modification of the lipid molecular structure, the formation and subsequent characterization of cylindrical microstructures, the use of these structures as templates for metallization, and the characterization and assessment of these hollow metal microcylinders for several potential applications.
Choices for the epitaxial growth of GalnP/GaAs dual junction concentrator solar cells (2005)

In this work we analyse the different possibilities that the GalnP/GaAs double junction solar cell structures grow by MOCVD offers, so as to achieve energy conversion efficiencies higher than 30 %. These possibilities comprise changes in the configuration of layers as well as the variation of the different parameters that affect the epitaxy process, such as temperature, growing time, dopants, partial pressure of gases, etc.
Probing the Mechanism of CRP Activation by Site-Directed Mutagenesis: The Role of Serine 128 in the Allosteric Pathway of cAMP Receptor Protein Activation (1995)

Upon activation by cAMP, Escherichia coli cAMP receptor protein (CRP) controls the expression of a network of catabolite sensitive genes. The activation of CRP by cAMP involves conformational changes such as realignments between subunits and domains within the protein. To understand the molecular events that lead to the activation of CRP, point mutations at position 128 were introduced via site-directed mutagenesis in an attempt to specifically affect the subunit interfacial interactions, as well as the ligand-binding reaction. The biochemical and biophysical properties of these mutants were rigorously tested with the goal of identifying the partial reactions in the activation pathway that are perturbed by this specific mutation. Results from this study suggest that mutation of Ser 128 to Ala or Pro does not significantly disturb the overall secondary structure as monitored by circular dichroism. The energetics of subunit-subunit interaction and protein stability were monitored by sedimentation and spectroscopic techniques. Although these mutants were designed to interrupt intersubunit interactions, the energetics of subunit association and protein stability remain quantitatively the same as those of the wild-type CRP. Nevertheless, the ability of the subunit to be realigned to the DNA-binding form is significantly affected as reflected by the pronounced decrease in the susceptibility of mutant CRP to proteolytic digestion in the presence of cAMP. In addition, the binding affinity of cAMP to the first ligand site in mutants S128A and S128P is the same as that of the wild type, but the affinity to the second ligand site is reduced. This observation indicates that mutation at position 128 affects ligand binding by amplifying the magnitude of negative cooperativity. Mutation at residue 128 does not impair the ability of interdomain interactions as indicated by the quantitative response of a spectroscopic probe in the DNA-binding domain to the binding of cAMP to the ligand-binding domain. The S128A mutant binds to a specific DNA sequence about 50-fold weaker than the wild-type CRP, while the mutant S128P has no measurable DNA affinity under the same conditions. This observation is consistent with the in vivo result that both mutants display an inactive CRP phenotype (CRP-). In summary, these results suggest that communication between domains induced by cAMP binding can be dissociated from the proper subunit realignment of the CRP dimer that is crucial for the activation of CRP.(ABSTRACT TRUNCATED AT 400 WORDS)
Bias compensation for the bearings-only pseudolinear target track estimator (2005)

The bearings-only pseudolinear target track estimator is known to suffer from severe bias problems. This paper presents a bias analysis for the pseudolinear estimator and develops a method of bias compensation, resulting in a closed-form reduced-bias pseudolinear estimator. The reduced-bias estimator is then incorporated into an instrumental variable estimator to produce asymptotically unbiased target motion parameter estimates. Unlike batch iterative estimators, the proposed instrumental variable estimator has a closed-from solution and therefore avoids the convergence problems associated with iterative estimators. The performance of the proposed instrumental variable estimator is illustrated by way of simulation examples and is shown to be almost identical to that of the computationally more demanding iterative maximum likelihood estimator.
Imaging Electromigration during the Formation of Break Junctions (2007)

Using a scanning electron microscope, we make real-time movies of gold nanowires during the process of electromigration. We confirm the importance of using a small series resistance when employing electromigration to make controlled nanometer-scale gaps suitable for molecular-electronics studies. We are also able to estimate the effective temperature experienced by molecular adsorbates on the nanowire during the electromigration process.
Study of a novel planar transmission line (2002)

A new type of monolithic planar transmission line, the microshield line, is proposed. This line can operate without the need for via-holes or the use of air-bridges for ground equalization. Furthermore, it has shown the tendency to radiate less than the conventional microstrip or coplanar waveguide (CPW) and can provide a wide range of impedances due to the many available parameters for design. The space domain integral equation method is used to analyze four different discontinuities of the proposed type. A comparison to conventional CPW with respect to radiation shows very good performance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Room-temperature electron mobility in strained Si/SiGe heterostructures (1993)

We report on room-temperature electron transport measurements in modulation-doped strained Si/SiGe heterostructures, grown by ultrahigh-vacuum chemical vapor deposition. A high room-temperature mobility is expected in such samples because of the strain-induced splitting of the conduction band in the silicon channel. Record values of over 2600 cm2/V s have been measured, almost twice the theoretical maximum for relaxed silicon.
Therapeutic Antibodies: Current State and Future Trends – Is a Paradigm Change Coming Soon? (2008)

Antibody-based therapeutics currently enjoy unprecedented success, growth in research and revenues, and recognition of their potential. It appears that the promise of the "magic bullet" has largely been realized. There are currently 22 monoclonal antibodies (mAbs) approved by the United States Food and Drug Administration (FDA) for clinical use and hundreds are in clinical trials for treatment of various diseases including cancers, immune disorders, and infections. The revenues from the top five therapeutic antibodies (Rituxan, Remicade, Herceptin, Humira, and Avastin) nearly doubled from $6.4 billion in 2004 to $11.7 billion in 2006. During the last several years major pharmaceutical companies raced to acquire antibody companies, with a recent example of MedImmune being purchased for $15.6 billion by AstraZeneca. These therapeutic and business successes reflect the major advances in antibody engineering which have resulted in the generation of safe, specific, high-affinity, and non-immunogenic antibodies during the last three decades. Currently, second and third generations of antibodies are under development, mostly to improve already existing antibody specificities. However, although the refinement of already known methodologies is certainly of great importance for potential clinical use, there are no conceptually new developments in the last decade comparable, for example, to the development of antibody libraries, phage display, domain antibodies (dAbs), and antibody humanization to name a few. A fundamental question is then whether there will be another change in the paradigm of research as happened 1-2 decades ago or the current trend of gradual improvement of already developed methodologies and therapeutic antibodies will continue. Although any prediction could prove incorrect, it appears that conceptually new methodologies are needed to overcome the fundamental problems of drug (antibody) resistance due to genetic or/and epigenetic alterations in cancer and chronic infections, as well as problems related to access to targets and complexity of biological systems. If new methodologies are not developed, it is likely that gradual saturation will occur in the pipeline of conceptually new antibody therapeutics. In this scenario we will witness an increase in combination of targets and antibodies, and further attempts to personalize targeted treatments by using appropriate biomarkers as well as to develop novel scaffolds with properties that are superior to those of the antibodies now in clinical use.
ARA Criteria Rheumatoid Arthritis (1989)

Journal Article ARA Criteria Rheumatoid Arthritis Get access J. EDWARDS J. EDWARDS Department of Rheumatology Research, Arthur Stanley HouseTottenham Street, London Wl 9PG, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Rheumatology, Volume 28, Issue 2, April 1989, Pages 176–177, https://doi.org/10.1093/rheumatology/28.2.176 Published: 01 April 1989 Article history Received: 09 November 1988 Published: 01 April 1989
Assembly of transgenic human P301S Tau is necessary for neurodegeneration in murine spinal cord (2019)

A pathological pathway leading from soluble monomeric to insoluble filamentous Tau is characteristic of many human neurodegenerative diseases, which also exhibit dysfunction and death of brain cells. However, it is unknown how the assembly of Tau into filaments relates to cell loss. To study this, we first used a mouse line transgenic for full-length human mutant P301S Tau to investigate the temporal relationship between Tau assembly into filaments, assessed using anti-Tau antibody AT100, and motor neuron numbers, in the lumbar spinal cord. AT100 immunoreactivity preceded nerve cell loss. Murine Tau did not contribute significantly to either Tau aggregation or neurodegeneration. To further study the relevance of filament formation for neurodegeneration, we deleted hexapeptides <sup>275</sup>VQIINK<sup>280</sup> and <sup>306</sup>VQIVYK<sup>311</sup>, either singly or in combination, from human 0N4R Tau with the P301S mutation. These hexapeptides are essential for the assembly of Tau into filaments. Homozygous mice transgenic for P301S Tau with the hexapeptide deletions, which expressed Tau at a similar level to the heterozygous line transgenic for P301S Tau, had a normal lifespan, unlike mice from the P301S Tau line. The latter had significant levels of sarkosyl-insoluble Tau in brain and spinal cord, and exhibited neurodegeneration. Mice transgenic for P301S Tau with the hexapeptide deletions failed to show significant levels of sarkosyl-insoluble Tau or neurodegeneration. Recombinant P301S Tau with the hexapeptide deletions failed to form β-sheet structure and filaments following incubation with heparin. Taken together, we conclude that β-sheet assembly of human P301S Tau is necessary for neurodegeneration in transgenic mice.
Adaptive-critic-based neural networks for aircraft optimal control (1996)

A dual neural network architecture for the solution of aircraft control problems is presented. The neural network structure, consisting of an action network and a critic network, is used to approximately solve the dynamic programming equations associated with optimal control with a high degree of accuracy. Numerical results from applying this methodology to optimally control the longitudinal dynamics of an aircraft are presented. The novelty in this synthesis of the optimal controller network is that it needs no external training inputs; it needs no a priori knowledge of the form of control. Numerical experiments with neural-network-based control as well as other pointwise optimal control techniques are presented. These results show that this network architecture yields optimal control over the entire range of training. In other words, the neural network can function as an autopilot. A scalar problem is also used in this study for easier illustration of the solution development.
Synthese und Halbleitereigenschaften arylsubstituierter Imidazole (1967)

Eine Anzahl tri- und tetrasubstituierter Mono- und Diimidazole wurde synthetisiert und die Temperatur-Abhängigkeit ihrer Gleichstrom-Dunkelleitfähigkeit gemessen. Die Beziehungen zwischen der Leitfähigkeit, ihrer Aktivierungs-Energie, der UV-Absorption, der Zahl der π-Elektronen und dem räumlichen Bau der Verbindungen werden erörtert.
Morphological, biochemical, and electrophysiological characterization of a clonal cell (H9c2) line from rat heart. (1991)

Morphological, electrophysiological, and biochemical properties of H9c2 cells, a permanent cell line derived from rat cardiac tissue, were studied. Although the lectin binding pattern revealed similar sugar residues in the surface coat of H9c2 cells and isolated rat cardiocytes, heart-specific morphological structures could not be detected in H9c2 cells. Under physiological ionic conditions, H9c2 cells exhibited an outwardly rectifying, transient K+ current. When this current component was blocked by Ba2+ and Cs+, we observed an inward current through Ca2+ channels (15.8 +/- 2.2 pA/pF, n = 18, measured as Ba2+ current) that showed all characteristics of cardiac L-type currents. The activation kinetics were fast, and the current was stimulated by isoproterenol. The effect of isoproterenol was mimicked by forskolin or intracellularly applied cAMP. In radioligand binding experiments, we identified a specific, saturable, stereoselective and reversible high-affinity [3H]-(+)PN 200-110 binding with a dissociation constant Kd = 0.53 +/- 0.28 nM and a maximal specific binding of Bmax = 129.3 +/- 16.1 fmol/mg protein. There was an additional low-affinity/high-capacity binding site, which is unlikely to be related to a Ca2+ channel protein. Signal-transducing G proteins in membranes were characterized by [32P]ADP-ribosylation catalyzed by bacterial toxins and by the use of various antibodies. Cholera toxin substrates of 42 and 45 kd were identified that apparently correlated to Gs alpha-subunits. Pertussis toxin substrates of 40-41 kd were tentatively identified as Gi alpha-subunits. The G protein Go was absent or at least extremely low in concentration.
Chronic nitric oxide inhibition with l-NAME: effects on autonomic control of the cardiovascular system (1998)

To determine whether increased sympathetic activity contributes to the hypertension induced by chronic exposure to moderate nitric oxide synthase (NOS) inhibition, various indexes of autonomic function were measured in rats given the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 10 mg/100 ml, approximately equal to 16 mg.kg-1.day-1) in the drinking water. One week of treatment raised blood pressure (139 +/- 3 vs. 106 +/- 1 mmHg; P < 0.01) and lowered heart rate (319 +/- 4 vs. 379 +/- 6 beats/min, P < 0.01). L-NAME had no effect on cardiac sympathetic tone, but elevated cardiac parasympathetic tone (-73 +/- 4 vs. -56 +/- 7 beats/min; P < 0.05). Depressor responses to ganglionic blockade were greater in L-NAME-treated rats (-50 +/- 5 vs. -34 +/- 5 mmHg; P < 0.05), whereas resting plasma, renal, and adrenal catecholamine values did not differ between groups. Treated rats also showed evidence of reduced baroreflex sympathetic stimulation of heart rate during hypotension and reduced parasympathetic activation during hypertension. Together, these data provide only very limited, indirect evidence that sympathetic stimulation contributes to the hypertension associated with moderate NOS inhibition.