gebhart/patrepeval huggingface dataset:
Rapid and simple LC‐MS/MS screening of 64 novel psychoactive substances using dried blood spots (2013)

The range of novel psychoactive substances (NPS) including phenethylamines, cathinones, piperazines, tryptamines, etc. is continuously growing. Therefore, fast and reliable screening methods for these compounds are essential and needed. The use of dried blood spots (DBS) for a fast straightforward approach helps to simplify and shorten sample preparation significantly. DBS were produced from 10 µl of whole blood and extracted offline with 500 µl methanol followed by evaporation and reconstitution in mobile phase. Reversed-phase chromatographic separation and mass spectrometric detection (RP-LC-MS/MS) was achieved within a run time of 10 min. The screening method was validated by evaluating the following parameters: limit of detection (LOD), matrix effect, selectivity and specificity, extraction efficiency, and short-term and long-term stability. Furthermore, the method was applied to authentic samples and results were compared with those obtained with a validated whole blood method used for routine analysis of NPS. LOD was between 1 and 10 ng/ml. No interference from matrix compounds was observed. The method was proven to be specific and selective for the analytes, although with limitations for 3-FMC/flephedrone and MDDMA/MDEA. Mean extraction efficiency was 84.6 %. All substances were stable in DBS for at least a week when cooled. Cooling was essential for the stability of cathinones. Prepared samples were stable for at least 3 days. Comparison to the validated whole blood method yielded similar results. DBS were shown to be useful in developing a rapid screening method for NPS with simplified sample preparation.
Recent Advances in Seismic Monitoring of Thermal EOR (2014)

Abstract Well-planned and executed reservoir surveillance has proven to add significantly to the production and ultimate recovery of hydrocarbons, notably in areas of Improved and Enhanced Oil Recovery (IOR/EOR). Recent technological advances in the area of data acquisition and integration have led to increased use of well and reservoir surveillance data to optimize such processes. In the case of thermal EOR, one of the most important subsurface uncertainties impacting performance is heat and steam front conformance, both vertically and arealy. This paper illustrates new geophysical technologies used for monitoring various thermal EOR recovery strategies in The Netherlands, Canada, and Oman. We focus on permanently buried seismic sources and receivers, refraction seismic, down-hole seismic, and the newly developed Distributed Acoustic Sensing (DAS) to enable low-cost and non-intrusive seismic surveillance. These technologies are not without challenges, but our field trials indicate they have the potential to broaden the successful application of reservoir monitoring onshore. Introduction Enhanced Oil Recovery (EOR) is well established as a tool for increasing recovery beyond secondary production, extending the life of fields, and postponing field abandonment activities and costs. Thermal EOR in particular has been instrumental to unlocking resources that may not be producible otherwise (e.g., large bitumen resources in Canada), and increasing recovery from heavy oil fields that have already undergone waterflood. Currently, thermal EOR is done only onshore, and for relatively shallow reservoirs (e.g., up to 1 km depth - a limitation imposed by heat losses that would condense steam to hot water for deeper injection wells). To optimize EOR, one must monitor reservoir changes induced by the treatment. Seismic monitoring is attractive for its ability to sense a large volume of the subsurface, illuminating it between and away from existing wells. It has proven useful for reservoir monitoring offshore (e.g., Hatchel et al., 2013; Stammeijer et al., 2013; El Ouair and Strønen, 2006; Osdal et al., 2006; Howie et al., 2005; Whitcombe et al., 2004). However, it has been slow to mature onshore, both because the Value-of-Information hurdle is higher in fields with high well density (typical for mature onshore developments) and because of a number of technical and non-technical challenges. The technical challenges include spatial and temporal variability of the near surface, changes in source and receiver coupling to the formation, complex and evolving infrastructure, high noise levels caused by activities above ground, and the relatively high cost of seismic operations in forested or populated areas. Non-technical challenges may include land access (e.g., restrictions due to competition with other uses such as farming or aboriginal activities), regulatory and environmental issues (surface footprint) and public perception of risk.
Sensor Fault Detection via Multiscale Analysis and Nonparametric Statistical Inference (1998)

Sensor validation is a topic of widespread importance. A new approach to sensor validation in real time is described that is based on (1) representation of the sensor signal by wavelets, (2) decomposition of the signal into different frequency ranges, (3) calculation of useful features of the signal at different frequencies, and (4) diagnosis of faulty operation via nonparametric statistical tests. The proposed strategy is able to isolate the effect of noise and process changes from the effects of physical changes in the sensor itself. To clarify the circumstances under which the above strategy could be used, a noisy signal from a simulated thermocouple in a dynamic continuous nonlinear unsteady state stirred tank reactor (CSTR) was analyzed. Faults were introduced into the thermocouple, and the diagnosis was carried out. The results of the diagnosis indicated that the proposed strategy had low type I (false alarm) and type II (failure to detect faults) errors and was distinctly better than a standard test for changes in a nonstationary signal of unknown characteristics.
Bias-Induced Photoluminescence Quenching of Single Colloidal Quantum Dots Embedded in Organic Semiconductors (2007)

We demonstrate reversible quenching of the photoluminescence from single CdSe/ZnS colloidal quantum dots embedded in thin films of the molecular organic semiconductor N,N'-diphenyl-N,N'-bis(3-methylphenyl)-(1,1'-biphenyl)-4,4'-diamine (TPD) in a layered device structure. Our analysis, based on current and charge carrier density, points toward field ionization as the dominant photoluminescence quenching mechanism. Blinking traces from individual quantum dots reveal that the photoluminescence amplitude decreases continuously as a function of increasing forward bias even at the single quantum dot level. In addition, we show that quantum dot photoluminescence is quenched by aluminum tris(8-hydroxyquinoline) (Alq3) in chloroform solutions as well as in thin solid films of Alq3 whereas TPD has little effect. This highlights the importance of chemical compatibility between semiconductor nanocrystals and surrounding organic semiconductors. Our study helps elucidate elementary interactions between quantum dots and organic semiconductors, knowledge needed for designing efficient quantum dot organic optoelectronic devices.
Antagonism of the Sodium-Potassium ATPase Impairs Chikungunya Virus Infection (2016)

Mitigation of disease induced by globally spreading, mosquito-borne arthritogenic alphaviruses requires the development of new antiviral strategies. High-throughput screening of clinically tested compounds provides a rapid means to identify undiscovered, antiviral functions for well-characterized therapeutics and illuminate host pathways required for viral infection. Our study describes the potent inhibition of Chikungunya virus and related alphaviruses by the cardiac glycoside digoxin and demonstrates a function for the sodium-potassium ATPase in Chikungunya virus infection.
Array-based vapor sensing using chemically sensitive, polymer composite resistors (1997)

We describe herein the construction of simple, low-power, broadly responsive vapor sensors. Insulating polymer-conductor composites have been shown to swell reversibly upon exposure to vapors. Thin films of polymer composites have been deposited across two metallic leads, with swelling-induced resistance changes of the films signaling the presence of vapors. To identify and classify vapors, arrays of such vapor-sensing elements have been constructed, with each element containing either carbon black or poly(pyrrole) as the conducting phase mixed with one of several different organic polymers as the insulating phase. A convenient chemical polymerization of poly(pyrrole) which allows a high degree of processibility is also described. The differing gas-solid partition coefficients for the various polymers of the sensor array produce a pattern of resistance changes that can be used to classify vapors and vapor mixtures. This type of sensor array has been shown to resolve common organic solvents, including molecules of different classes (such as aromatics from alcohols) as well as those within a particular class (such as benzene from toluene and methanol from ethanol). The response of an individual composite to varying concentrations of solvent is shown to be consistent with the predictions of percolation theory. Accordingly, significant increases in the signals of array elements have been observed for carbon black-polymer composites that were operated near their percolation thresholds.
Mesenchymal Stem/Stromal Cells in Stromal Evolution and Cancer Progression (2015)

The study of cancer biology has mainly focused on malignant epithelial cancer cells, although tumors also contain a stromal compartment, which is composed of stem cells, tumor-associated fibroblasts (TAFs), endothelial cells, immune cells, adipocytes, cytokines, and various types of macromolecules comprising the extracellular matrix (ECM). The tumor stroma develops gradually in response to the needs of epithelial cancer cells during malignant progression initiating from increased local vascular permeability and ending to remodeling of desmoplastic loosely vascularized stromal ECM. The constant bidirectional interaction of epithelial cancer cells with the surrounding microenvironment allows damaged stromal cell usage as a source of nutrients for cancer cells, maintains the stroma renewal thus resembling a wound that does not heal, and affects the characteristics of tumor mesenchymal stem/stromal cells (MSCs). Although MSCs have been shown to coordinate tumor cell growth, dormancy, migration, invasion, metastasis, and drug resistance, recently they have been successfully used in treatment of hematopoietic malignancies to enhance the effect of total body irradiation-hematopoietic stem cell transplantation therapy. Hence, targeting the stromal elements in combination with conventional chemotherapeutics and usage of MSCs to attenuate graft-versus-host disease may offer new strategies to overcome cancer treatment failure and relapse of the disease.
Efficient extracavity generation of radially and azimuthally polarized beams (2007)

We demonstrate an efficient transformation of a linearly polarized Gaussian beam to a radially or an azimuthally polarized doughnut (0,1)* Laguerre-Gaussian beam of high purity. We use a spatially variable retardation plate, composed of eight sectors of a lambda/2 retardation plate, to transform a linear polarization distribution to radial/azimuthal distribution. We transformed an Nd:YAG Gaussian beam with M(2)=1.3 to a radially and azimuthally polarized (0,1)* Laguerre-Gaussian beams with M(2)=2.5 and degree of radial/azimuthal polarization of 96-98%.
Gene regulation of siderophore‐mediated iron acquisition in Pseudomonas: not only the Fur repressor (1995)

Pseudomonads have several siderophore-mediated iron-acquisition systems. These can be classified onto two groups: (i) the biosynthesis of a siderophore (iron-transport agent) followed by its uptake; and (ii) uptake of heterologous ferric-siderophores in which the siderophore is produced by other microbial species. The regulation of these mechanisms employs both positive and negative elements ensuring expression of the relevant genes only when they are absolutely required. Siderophore biosynthesis in induced in response to iron limitation. In contrast, activation of the heterologous transport systems is not only regulated by iron availability but also requires the presence of their cognate ferric-siderophores. The investigation of these regulation systems in three different Pseudomonas species gave similar results consisting of regulatory elements new to the field of iron regulation. These elements are superimposed upon the regulation by the Fur repressor, which in other bacteria directly regulate the expression of the iron-assimilation genes in response to iron availability.
Muhaddislerin Hadis İlmindeki Dereceleri ve Meşhur Hadis Âlimlerine Dair İki Önemli Kaynak (2004)

https://www.marife.org/marife/article/view/208
Performance evaluation of some clustering algorithms and validity indices (2002)

In this article, we evaluate the performance of three clustering algorithms, hard K-Means, single linkage, and a simulated annealing (SA) based technique, in conjunction with four cluster validity indices, namely Davies-Bouldin index, Dunn's index, Calinski-Harabasz index, and a recently developed index I. Based on a relation between the index I and the Dunn's index, a lower bound of the value of the former is theoretically estimated in order to get unique hard K-partition when the data set has distinct substructures. The effectiveness of the different validity indices and clustering methods in automatically evolving the appropriate number of clusters is demonstrated experimentally for both artificial and real-life data sets with the number of clusters varying from two to ten. Once the appropriate number of clusters is determined, the SA-based clustering technique is used for proper partitioning of the data into the said number of clusters.
Doxorubicin sensitizes human bladder carcinoma cells to Fas-mediated cytotoxicity (1997)

This study demonstrated that treatment of bladder carcinoma cells with doxorubicin sensitized the cells to lysis by anti-Fas MoAb. The synergistic effect obtained with established doxorubicin-resistant bladder carcinoma cells and freshly isolated bladder carcinoma cells suggests that drug-resistant bladder carcinoma cells can be sensitized by doxorubicin to Fas- and Fas ligant-mediated cytotoxicity by lymphocytes. Furthermore, the sensitization required low concentrations of doxorubicin, thus supporting the in vivo application of a combination of chemotherapy and immunotherapy in the treatment of drug-resistant and/or immunotherapy-resistant bladder carcinoma.
Conversion of glutaconate CoA‐transferase from Acidaminococcus fermentans into an acyl‐CoA hydrolase by site‐directed mutagenesis (1997)

The heterooctameric (alphabeta)4 glutaconate CoA-transferase (EC 2.8.3.12) from the anaerobic bacterium Acidaminococcus fermentans catalyses the transfer of CoASH from acetyl-CoA to the 1-carboxylate of glutaconate. During this reaction the glutamate residue 54 of the beta-subunit (betaE54) forms a CoA-ester. The single amino acid replacement betaE54D resulted in a drastic change of enzymatic function. The CoA-transferase activity decreased from 140 to less than 0.01 s(-1), whereas the acyl-CoA hydrolase activity increased from less than 0.01 to 16 s(-1). The new enzyme was able to catalyse the hydrolysis of glutaryl-CoA, acetyl-CoA and 3-butenoyl-CoA. Since the mutants betaE54A and betaE54N showed neither acyl-CoA hydrolase nor CoA-transferase activity, it was concluded that the aspartate carboxylate of the mutant betaE54D acted as a general base which facilitated the attack of water at the thiolester carbonyl. Surprisingly, Km for glutaryl-CoA hydrolysis by the mutant (0.7 microM) as compared to CoA-transfer by the wild-type (28 microM) was 40 times lower. A 65 kDa protein, obtained by fusing the genes, gctA-gctB, coding for glutaconate CoA-transferase, retained 30% of the wild-type activity. Comparison of the amino acid sequences of 13 related enzymes demonstrated that Nature already has applied gene fusion in the case of pig heart CoA-transferase and has been using the E --> D mutation for catalysis by a yeast acetyl-CoA hydrolase.
Multidimensional Subcarrier Mapping for Bit-Interleaved Coded OFDM With Iterative Decoding (2007)

A power and bandwidth-efficient bit-interleaved coded modulation (BICM) with orthogonal frequency-division multiplexing (OFDM) and iterative decoding (BI-COFDM-ID) using combined multidimensional mapping and subcarrier grouping is proposed for broadband transmission in a frequency-selective fading environment. A tight bound on the asymptotic error performance is developed, which shows that subcarrier mapping and grouping have independent impacts on the overall error performance, and hence, they can be independently optimized. Specifically, it is demonstrated that the optimal subcarrier mapping is similar to the optimal multidimensional mapping for bit-interleaved coded modulation with iterative decoding (BICM-ID) in frequency-flat Rayleigh fading environment, whereas the optimal subcarrier grouping is the same with that of OFDM with linear constellation preceding (LCP). Furthermore, analytical and simulation results show that the proposed system with the combined optimal subcarrier mapping and grouping can achieve the full channel diversity without using LCP and provide significant coding gains as compared to the previously studied BI-COFDM-ID with the same power, bandwidth, and receiver complexity.
Calcium Based Test Method for Evaluation of Photovoltaic Edge-Seal Materials (2011)

Because of the sensitivity of some photovoltaic devices to moisture-induced corrosion, they are packaged using impermeable front- and back-sheets with an edge seal to prevent moisture ingress. Evaluation of edge seal materials can be difficult because of the low permeation rates involved and/or non-Fickian behavior. Here, using a Ca film deposited on a glass substrate, we demonstrate the evaluation of edge seal materials in a manner that effectively duplicates their use in a photovoltaic application and compare the results with standard methods for measuring water vapor transport. We demonstrate how moisture permeation data from polymer films can be used to estimate moisture ingress rates and compare the results of these two methods. Encapsulant materials were also evaluated for comparison and to highlight the need for edge seals. Of the materials studied, dessicant-filled polyisobutene materials demonstrate by far the best potential to keep moisture out for a 20 to 30 year lifetime.
Electrochemical Stability of Thin-Film LiCoO2 Cathodes by Aluminum-Oxide Coating (2003)

High-performance thin-film LiCoO2 cathodes were successfully fabricated by aluminum-oxide coating. Both the galvanostatic charge−discharge experiments and the cyclic voltammograms (CVs) showed enhanced electrochemical properties in the Al2O3-coated LiCoO2 films compared to those in the uncoated ones. The improved cycling behaviors in the coated samples are caused by the suppression of cobalt dissolution from the LiCoO2 thin films, with the formation of an aluminum-oxide solid electrolyte residing between the LiCoO2 cathode and liquid electrolyte. Galvanostatic intermittent titration technique (GITT) results clearly showed that the Al2O3-coated samples had higher Li diffusivities than the uncoated ones after 80 cycles. The effect of Al2O3 thickness on the electrochemical properties up to 300 nm was also studied.
Swelling and Mechanical Properties of Modified HEMA-based Superporous Hydrogels (2010)

Superporous hydrogels (SPHs), based on poly(2-hydroxyethyl methacrylate) (PHEMA), were prepared by adding minute amounts of an ion-complexable hydrophilic acrylic acid. PHEMA SPHs are generally strong, but their swelling is minimal. To improve the swelling, different poly(HEMA-co-acrylic acid) hydrogels were polymerized and crosslinked, then physically treated with divalent calcium and trivalent aluminum cations. The incorporation of acrylic acid copolymer into the SPH, followed by crosslinking of the copolymer with calcium or aluminum ions produced SPHs with improved swelling and strength. Cells in the presence of hydrogel showed high viability indicating the absence of cytotoxicity and stimulatory effect.
Cytotoxic activity of lymphocytes. VI. Heterogeneity of cytotoxins in supernatants of mitogen-activated lymphocytes. (1976)

Two different lymphotoxins synthesized by human blood lymphocytes stimulated with phytohemagglutin (PHA) are described. The two toxins are called alpha-LT and beta-LT relative to their elution order on gel chromatography. Their m.w. are 75,000 and 45,000 daltons, respectively. Both toxins appear as early as 7 hr after the addition of PHA, with the amount of beta-toxin exceeding that of alpha-LT initially. Both toxins are differentiated from a third toxin (adherent cell toxin, ACT) made by plastic-adherent cells without requiring mitogen-stimulation. Depletion of macrophages or B cells does not affect the synthesis of either lymphotoxin. Monospecific antisera were elicited to alpha-LT. Antisera elicited to beta-LT also neutralized alpha-LT but to a significantly lesser degree. Alpha-LT appears to be the lymphotoxin described by most other workers. Beta-LT is unstable at 37 degrees C which may explain why the low m.w. lymphotoxin has not been described previously.
Secure coprocessor-based intrusion detection (2002)

The goal of an intrusion detection system (IDS) is to recognize attacks such that their exploitation can be prevented. Since computer systems are complex, there are a variety of places where detection is possible. For example, analysis of network traffic may indicate an attack in progress [11], a compromised daemon may be detected by its abnormal behavior [14, 12, 5, 10, 15], and subsequent attacks may be prevented by the detection of backdoors and stepping stones [16, 17].
Successful retroviral mediated transduction of a reporter gene in human dendritic cells: feasibility of therapy with gene-modified antigen presenting cells. (1997)

In this study we have analyzed the feasibility of gene transfer in human dendritic cells (DCs). DCs were generated from T and B cell-depleted peripheral blood mononuclear cells cultured for 7 days in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). The cells showed morphologic and immunophenotypical features typical of DCs, including expression of major histocompatibility complex (MHC) class I and II molecules, CD1a, CD80, CD86, CD13, CD33, CD40, and CD54. The cells showed high stimulatory activity in both allogeneic and autologous mixed lymphocyte reaction (MLR). The bacterial reporter gene lacZ coding for beta-galactosidase (beta-gal) was introduced in DCs by three sequential cycles of infection using a MFG retroviral vector system. After 7 days of culture 35-67% of the cells showed high expression of beta-gal activity, proving successful gene transfer. Stable integration of the lacZ gene was demonstrated by genomic DNA-polymerase chain reaction (PCR) up to 20 days after gene transfer. The percentage of transduction was similar when DCs were further purified by immunomagnetic separation according to CD1a-expression. We conclude that human DCs can be efficiently gene modified, further broadening the spectrum of possible DC-based clinical applications.