gebhart/patrepeval huggingface dataset:
Excitation of surface waves on conducting, stratified, dielectric-clad, and corrugated surfaces (1957)

An expressio n for t he fi eld of an elect ric dipole located over a fiat surface with a specifi ed surface impedance Z is d erived from t he form al integra l solution by a modified saddlepoint m ethod . Using t he value of Z appropriate for a homogeneous conducting g round, the general expressions reduce to those given bgr Norton. In this case the phase of Z li es between 0 a nd 45. When t he phase exceed s 45 , as it m ay for a stratified ground, t h e r adiated wave of the dipole becomes partially t rapp ed to t he interface. This effect is most pronounced for an inductive surface where t he phase of Z is 90 0 , in which case t he energy of the wave is confin ed within a sm all distance from t h e surface. Such inductivc surfaces are a metallic pla ne with a t hin dielectric film or a co rrugated s urface.
Do New Teaching Standards Undermine Performance on Old Tests? (1998)

As almost every state attempts to reform mathematics instruction by implementing new teaching standards, state testing practices remain largely unchanged. Do these new standards undermine student performance on old tests? This question is investigated by examining whether middle and high school algebra students taught in a manner consistent with the National Council for Teachers of Mathematics Professional Standards performed differently on three standardized algebra assessments than students taught in traditional classrooms. The data come from 94 teachers, 2,369 students, and 40 schools in one of the nation’s largest school districts. Results indicate that the Standards do not undermine performance on the old tests. In fact, middle school algebra students whose teachers spent more time using the NCTM teaching approach had higher growth rates than students whose teachers spent less time using the approach. However, students with higher ability levels benefited more. The growth rates of the lowest achieving students, the high school students (who are disproportionately Black and poor), were not helped or hindered by the NCTM teaching approach. If, as other studies indicate, the new standards help students on more novel tests, the finding that students benefit or at least are not hurt on traditional tests strengthens the case for implementing the NCTM reforms.
Rigid Multivalent Scaffolds Based on Adamantane (2008)

We present two new synthetic strategies to rigid multivalent scaffolds of the general structure 1 based on adamantane. Both routes start from arylated adamantane derivatives and give the target compounds 12 and 18 in 5 and 7 steps, respectively. These scaffolds have been designed for the assembly of multivalent binders for cell surface epitopes. The adamantane nucleus exposes three carboxylic acid groups in a well-defined tripodal geometry for conjugation of targeting ligands. In addition, an amino group at the fourth bridgehead position provides a flexible linker for attachment of effector molecules such as contrast agents, radiotracers, or cytotoxins without interfering with the cell binding process.
Single Molecule Dynamics Studied by Polarization Modulation (1996)

We observed and made unambiguous distinctions between abrupt photophysical events of single molecules: a rotational jump of a single dipole, a transition to a dark state (reversible and irreversible photobleaching), and a spectral jump. The study was performed in the far field by modulating the excitation polarization and monitoring the fluorescence in time. This technique also allowed us to measure the in-plane dipole orientation of stationary single molecular dipoles with subdegree accuracy and to resolve desorption and readsorption of fluorophores from and onto a glass surface. In one case, clear evidence was obtained for rapid rotation of the dipole after a desorption process.
Shaped crystal growth (1993)

Preface. Introduction. 1. Dynamic Stability of Crystallization - the Basis of Shaped Crystal Growth. 2. The Czochralski Technique. 3. Techniques of Pulling from Shaper. 4. The Verneuil Technique. 5. The Floating-Zone Technique. 6. Radial Instability of Vapor Whiskers. 7. Stability of Cylindrical Pore Growth. 8. Two Shaping Elements Technique. 9. Variable and Local Shaping Techniques. 10. Crystal Cross-Section Shapes. 11. Shaped Crystal Growth by TPS. 12. Crystallization under Microgravity Conditions. Appendix: Calculation of the Growth Angle from the Shape of a Crystallization Drop. References.
Establishing Preferred Product Characterization for the Evaluation of RNA Vaccine Antigens (2019)

The preferred product characteristics (for chemistry, control, and manufacture), in addition to safety and efficacy, are quintessential requirements for any successful therapeutic. Messenger RNA vaccines constitute a relatively new alternative to traditional vaccine development platforms, and thus there is less clarity regarding the criteria needed to ensure regulatory compliance and acceptance. Generally, to identify the ideal product characteristics, a series of assays needs to be developed, qualified and ultimately validated to determine the integrity, purity, stability, and reproducibility of a vaccine target. Here, using the available literature, we provide a summary of the array of biophysical and biochemical assays currently used in the field to characterize mRNA vaccine antigen candidates. Moreover, we review various in vitro functional cell-based assays that have been employed to facilitate the early assessment of the biological activity of these molecules, including the predictive immune response triggered in the host cell. Messenger RNA vaccines can be produced rapidly and at large scale, and thus will particularly benefit from well-defined and well-characterized assays ultimately to be used for in-process, release and stability-indications, which will allow equally rapid screening of immunogenicity, efficacy, and safety without the need to conduct often lengthy and costly in vivo experiments.
Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification (2002)

We describe a new method for relative quantification of 40 different DNA sequences in an easy to perform reaction requiring only 20 ng of human DNA. Applications shown of this multiplex ligation-dependent probe amplification (MLPA) technique include the detection of exon deletions and duplications in the human BRCA1, MSH2 and MLH1 genes, detection of trisomies such as Down's syndrome, characterisation of chromosomal aberrations in cell lines and tumour samples and SNP/mutation detection. Relative quantification of mRNAs by MLPA will be described elsewhere. In MLPA, not sample nucleic acids but probes added to the samples are amplified and quantified. Amplification of probes by PCR depends on the presence of probe target sequences in the sample. Each probe consists of two oligonucleotides, one synthetic and one M13 derived, that hybridise to adjacent sites of the target sequence. Such hybridised probe oligonucleotides are ligated, permitting subsequent amplification. All ligated probes have identical end sequences, permitting simultaneous PCR amplification using only one primer pair. Each probe gives rise to an amplification product of unique size between 130 and 480 bp. Probe target sequences are small (50-70 nt). The prerequisite of a ligation reaction provides the opportunity to discriminate single nucleotide differences.
Statistical parametric speech synthesis based on product of experts (2010)

Multiple-level acoustic models (AMs) are often combined in statistical parametric speech synthesis. Both linear and non-linear functions of the observation sequence are used as features in these AMs. This combination of multiple-level AMs can be expressed as a product of experts (PoE); the likelihoods from the AMs are scaled, multiplied together and then normalized. Currently these multiple-level AMs are individually trained and only combined at the synthesis stage. This paper discusses a more consistent PoE framework where the AMs are jointly trained. A generalization of trajectory HMM training can be used for multiple-level Gaussian AMs based on linear functions. However for the non-linear case this is not possible, so a scheme based on contrastive divergence learning is described. Experimental results show that the proposed technique provides both a mathematically elegant way to train multiple-level AMs and statistically significant improvements in the quality of synthesized speech.
Screen design for flat-panel imagers in diagnostic radiology (1998)

The image quality of Gd<SUB>2</SUB>O<SUB>2</SUB>S:Tb phosphor screens used in a flat-panel photodiode array system is examined. The presampled Modulation Transfer Function (MTF), the Normalized Noise Power Spectrum surface (NNPS) and the resulting Detective Quantum Efficiency (DQE) are discussed for a variety of screens used in such a system. A technique for extracting the limiting DQE for a system with significant electronic noise is described. This allows for the examination of the imaging performance of the X-ray converter (or phosphor screen) and removes issues of photodiode array and readout electronics performance. It is shown that depending on the metric being used to judge the imaging performance (i.e., MTF or DQE), it is possible to design a more optimal screen than those currently available for use in screen-film imaging. Evidence is also presented for a significant degradation of the DQE at higher spatial frequencies due to the variation in the light spread MTF through the depth of the screen.
Immune Responses to Reporter Proteins and High Viral Dose Limit Duration of Expression with Adenoviral Vectors: Comparison of E2a Wild Type and E2a Deleted Vectors (1997)

Experiments designed to evaluate the effect of deletion of E2a on duration of expression using adenoviral vectors led to a series of observations regarding host responses to adenoviral vectors and reporter proteins. In studies using human alpha1-antitrypsin (hAAT) as a reporter gene, we found that the duration of expression is very brief for C3H/J and CBA/J mice but is prolonged for C57BL/6J mice, that disappearance of hAAT from the blood is correlated with the appearance of antibodies, and that immunization against hAAT can prevent appearance of the protein in the blood after administration of an adenoviral vector. Deletion of E2a in hAAT vectors did not prolong expession in C3H/J or CBA/J mice and did not shorten duration of expression in C57BL/6J mice. Using similar vectors expressing Escherichia coli beta-galactosidase (beta-Gal) in immunocompetent mice, short duration of expression with a beta-Gal reporter was remarkably different from the long expression with an identical vector expressing hAAT in C57BL/6J. In the case of vectors expressing hAAT, adenoviral sequences persisted in the liver, and inflammatory responses were minimal compared to vectors expressing beta-Gal, where adenoviral sequences disappeared from the liver concomitant with a prominent inflammatory response. The duration of expression of beta-Gal in hepatocytes was increased in transgenic mice expressing the reporter in keratinocytes, indicating that host immune responses to the reporter can limit duration of expression. Dosage studies indicated that persistence of expression of hAAT can be markedly decreased by administration of high doses of vector in a manner consistent with a nonimmune-mediated toxicity following injection. These experiments indicate that host responses to reporter genes rather than host responses to adenoviral proteins can be the primary determinant of duration of expression under many experimental conditions.
ACR and EULAR improvement criteria have comparable validity in rheumatoid arthritis trials. American College of Rheumatology European League of Associations for Rheumatology. (1999)

We compared the validity of the American College of Rheumatology (ACR) and the European League of Associations for Rheumatology (EULAR) definitions of response in rheumatoid arthritis (RA) clinical trials. US: ACR and EULAR improvement criteria were calculated in 7 large randomized RA clinical trials. The discriminant validity of the response criteria between treatment groups was studied using the Mantel-Haenszel chi-squared value. To compare both sets of criteria the chi-squared ratio was determined for each trial. Europe: In 2 large randomized RA clinical trials, ACR and EULAR criteria were calculated, once with extensive and once with 28 joint counts. The classification of patients with these 4 criteria were compared with each other using cross tables. We further studied the difference in response between treatment groups per trial, the association of response with patient and investigator assessment of improvement, and the association of response with radiological progression. US: The chi-squared ratio for most trials was close to 1. There was no clear pattern suggesting that the discriminant validity of the ACR criteria was stronger than the discriminant validity of the EULAR definition of response or vice versa. Europe: Conflicting results between ACR and EULAR were present in only 3% of patients in both trials. The discriminant validity of all 4 criteria (ACR and EULAR with reduced and extensive joint counts) was comparable. All criteria were related with the overall assessment of improvement by both investigator and patient. The association with radiographic progression was comparable for EULAR and ACR improvement criteria. There is a high level of agreement between ACR and EULAR improvement classification, and their validity is equivalent. The discriminating potential of the criteria between treatment groups is comparable, as is the association with patient's and investigator's overall assessment and with radiographic progression.
Autologous stromal vascular fraction therapy for rheumatoid arthritis: rationale and clinical safety (2012)

Advancements in rheumatoid arthritis (RA) treatment protocols and introduction of targeted biological therapies have markedly improved patient outcomes, despite this, up to 50% of patients still fail to achieve a significant clinical response. In veterinary medicine, stem cell therapy in the form of autologous stromal vascular fraction (SVF) is an accepted therapeutic modality for degenerative conditions with 80% improvement and no serious treatment associated adverse events reported. Clinical translation of SVF therapy relies on confirmation of veterinary findings in targeted patient populations. Here we describe the rationale and preclinical data supporting the use of autologous SVF in treatment of RA, as well as provide 1, 3, 6, and 13 month safety outcomes in 13 RA patients treated with this approach.
In SituElectron Microscopy Studies of the Sintering of Palladium Nanoparticles on Alumina during Catalyst Regeneration Processes (2004)

Sintering of a palladium catalyst supported on alumina (Al2O3) in an oxidizing environment was studied by in situ transmission electron microscopy (TEM). In the case of a fresh catalyst, sintering of Pd particles on an alumina surface in a 500 mTorr steam environment happened via traditional ripening or migration and coalescence mechanisms and was not significant unless heating above 500 degrees C. After the catalyst was used for the hydrogenation of alkynes, TEM coupled with convergent beam electron diffraction and electron energy loss spectroscopy analysis revealed that most of the Pd particles were lifted from the alumina surface by hydrocarbon buildup. This dramatically different morphology totally changed the sintering mechanism of Pd particles during the regeneration process. Catalytic gasification of hydrocarbon around these particles in an oxidizing environment allowed the Pd particles to move around and coalesce with each other at temperatures as low as 350 degrees C. For catalysts heating under 500 mTorr steam at 350 degrees C, steam stripped hydrocarbon catalytically at the beginning, but the reaction stopped after 4 h. Heating in air resulted in both catalytic and noncatalytic stripping of hydrocarbon.
Antisense Aims for a Renaissance (1997)

CAMBRIDGE, MASSACHUSETTS-- Once plagued by side effects and mysterious failures, antisense technologies are now shedding their troubled image. Basic research has uncovered explanations--and solutions--to some of the earlier problems, and antisense drugs have shown promise in early clinical trials against cancer and Crohn9s disease.
Molecular design for missile drug: Synthesis of adriamycin conjugated with immunoglobulin G using poly(ethylene glycol)‐block‐poly(aspartic acid) as intermediate carrier (1989)

Abstract Synthesis of a novel conjugate of anticancer drug adriamycin, poly(ethylene glycol)‐ block ‐poly(aspartic acid), and immunoglobulin G is reported. Poly(ethylene glycol)‐ block ‐poly(aspartic acid), binding adriamycin on the poly(aspartic acid) chain, showed an excellent water‐solubility despite the introduction of a large amount of strongly hydrophobic adriamycin, and was found to form a micellar structure in aqueous solution. Efficient coupling between poly(ethylene glycol)‐ block ‐poly(aspartic acid) binding adriamycin and immunoglobulin G was achieved via disulfide linkages.
Mutational analysis of loxP sites for efficient Cre‐mediated insertion into genomic DNA (2003)

The Cre/loxP system has been used in transgenic models primarily to excise DNA flanked by loxP sites for gene deletion. However, the insertion reaction is more difficult to control since the excision event is kinetically favored. Mutant loxP sites favoring integration were identified using a novel, bacterial screening system. Utilizing lambda integrase, mutant loxP sites were placed at the E. coli attB site and the excision-insertion ratios of incoming DNA plasmids carrying a second, complementary mutant loxP site were determined. Comparison of 50 mutant loxP sites combinations to the native loxP site revealed that mutations to the inner 6 bp of the Cre binding domain severely inhibited recombination, while those in the outer 8 bps were more tolerated. The most efficient loxP combinations resulted in 1421-fold and 1529-fold increases in relative integration rates over wild-type loxP sites. These loxP mutants could be exploited for site-directed "tag and insert" recombination experiments.
Role of L-selectin in the development of autoimmune diabetes in non-obese diabetic mice (2004)

Autoimmune diabetes is characterized by an early mononuclear infiltration of pancreatic islets and later selective autoimmune destruction of insulin-producing beta cells. Lymphocyte homing receptors have been considered candidate targets to prevent autoimmune diabetes. L-selectin (CD62L) is an adhesion molecule highly expressed in naive T and B cells. It has been reported that blocking L-selectin in vivo with a specific antibody (Mel-14) partially impairs insulitis and diabetes in autoimmune diabetes-prone non-obese diabetic (NOD) mice. In the present study we aimed to elucidate whether genetic blockade of leukocyte homing into peripheral lymph nodes would prevent the development of diabetes. We backcrossed L-selectin-deficient mice onto the NOD genetic background. Surprisingly NOD/L-selectin-deficient mice exhibited unaltered islet mononuclear infiltration, timing of diabetes onset and cumulative incidence of spontaneous diabetes when compared to L-selectin-sufficient animals. CD4, CD8 T cells and B cells were present in islet infiltrates from 9-week-old L-selectin-sufficient and -deficient littermates. Moreover, total splenocytes from wild-type, heterozygous or NOD/L-selectin-deficient donor mice showed similar capability to adoptively transfer diabetes into NOD/SCID recipients. On the other hand, homing of activated, cloned insulin-specific autoaggressive CD8 T cells (TGNFC8 clone) is not affected in NOD/L-selectin-deficient recipients. We conclude that L-selectin plays a small role in the homing of autoreactive lymphocytes to regional (pancreatic) lymph nodes in NOD mice.
Rude unhinging of the machinery of life: metabolic approaches to hemorrhagic shock (2001)

In 1862, Samuel Gross described shock as the "rude unhinging" of the machinery of life. As noted above, adequate oxygen delivery and metabolism are essential to the maintenance of cellular energy stores. Failure of adequate tissue oxygen delivery and utilization during shock can lead to organ dysfunction and death. Hemorrhagic shock after trauma can result in inherent mitochondrial dysfunction as manifested by decoupling. This pathologic condition has been recently termed cytopathic hypoxia. Since mitochondria are the ultimate consumer of oxygen in cells, mitochondria might indeed be the machinery of life rudely unhinged by shock. Yet, therapeutic strategies have been recently developed to support mitochondrial function in shock and related states. If these therapeutic interventions directed towards organelle and cellular resuscitation are proven to enhance human organ function and improve survival, then these strategies could augment current therapeutic regimens directed exclusively towards hemodynamic and ventilatory homeostasis.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies. (1984)

Mouse monoclonal antibodies to several cell surface antigens of human ovarian and endometrial carcinomas have been produced. The distribution of the antigens was determined by mixed hemagglutination assays on 153 normal and malignant cell cultures of various types and by immuno-peroxidase staining of frozen sections of 27 normal adult and 24 fetal tissues. Five distinct antigens were characterized. MD144 antigen was detected on only a single ovarian carcinoma cell line and has the biochemical properties of a lipid. MH55 antigen is weakly expressed on ovarian and uterine cancer cell lines but not on other cells and tissues tested. MF61 antigen was detected on an ovarian carcinoma and some renal carcinoma cell lines but not on other cell lines tested. It was also detected by immunoperoxidase staining in the noncellular follicles of the thyroid and in uterine glandular epithelial cells. This antigen also has the properties of a lipid. MF116 antigen was detected on a proportion of ovarian, uterine, renal, and bladder carcinoma and neuroblastoma cell lines and on normal kidney epithelial cell cultures but not on other cell lines tested. It was not detected in sections of any normal tissue tested using the immunoperoxidase method. MF116 was readily detected in the spent culture medium but not in detergent-solubilized extracts of metabolically radiolabeled cells. This shed antigen is a glycoprotein of Mr 105,000 and isoelectric point lower than pH 4.0. MH94 antigen was detected on a proportion of ovarian, uterine, colon, breast, lung, cervical, and pancreatic carcinoma cell lines. In tissue sections it was detected in many but not all epithelia, predominantly in secretory epithelial cells. Antibody MH94 did not immunoprecipitate a detectable antigen.
Inducing achiral aliphatic oligoureas to fold into helical conformations (2014)

The ability of urea-linked oligomers of achiral diamines (achiral analogues of the well-established chiral oligourea foldamers) to adopt helical conformations was explored spectroscopically. Up to four achiral units were ligated either to a well-formed helical trimer or to a single chiral diamine, and the extent to which they adopted a screw-sense preference was determined by NMR and CD. In the best performing cases, a trimeric chiral oligourea and even a single cis-cyclohexanediamine monomer induced folding into a helical conformation.