gebhart/patrepeval huggingface dataset:
Respiratory pump contributes to increased physiological reserve for compensation during simulated haemorrhage (2014)

Intrathoracic pressure regulation (IPR) represents a therapy for increasing systemic circulation through the creation of negative intrathoracic pressure. We hypothesized that using this 'respiratory pump' effect would slow the diminution of the physiological reserve to compensate during progressive reductions in central blood volume. The compensatory reserve index (CRI) algorithm was used to measure the proportion (from 100 to 0%) of reserve capacity that remained to compensate for central volume loss before the onset of cardiovascular decompensation. Continuous analog recordings of arterial waveforms were extracted from data files of seven healthy volunteers. Subjects had previously participated in experiments designed to induce haemodynamic decompensation (presyncope) by progressive reduction in central blood volume using graded lower-body negative pressure. The lower-body negative pressure protocol was completed while breathing spontaneously through a standard medical face mask without (placebo) and with a resistance (approximately -7 cmH2O; active IPR) applied during inspiration. At the onset of presyncope in the placebo conditions, CRI was smaller than the CRI observed at the same time point in the active IPR conditions. The CRI at the onset of presyncope during active IPR (0.08 ± 0.01) was similar to the CRI at presyncope with placebo. Kaplan-Meier and log rank tests indicated that CRI survival curves were shifted to the right by active IPR. Optimizing the respiratory pump contributed a small but significant effect of increasing tolerance to progressive reductions in central blood volume by extending the compensatory reserve.
Chemoselective Esterification of Phenolic Acids and Alcohols (2002)

[formula: see text] The Mitsunobu reaction can distinguish between alcohol and phenol hydroxyls in esterification reactions, providing an expeditious and broadly applicable entry into various phenolics and polyphenolics of biomedical and nutritional relevance.
Novel Building Blocks for Biomimetic Assemblies. Synthesis, Characterization, and Spectroscopic and Electrochemical Properties of New Bidentate Ligands Derived from Lysine and Cysteine and Their Complexes with Bis(2,2‘-bipyridine)ruthenium(II) (1996)

Synthetic amino acids suitable for the assembly of small, redox-active metallopeptides are described. Nα-((1,1-Dimethylethoxy)carbonyl)-Nε-(2-pyridylmethyl)-l-lysine (1), Nα-acetyl-S-(2-pyridylmethyl)-l-cysteine (2), and Nα-acetyl-S-(2-(2-pyridyl)ethyl)-l-cysteine (3) have been synthesized by alkylation of the Nα-protected amino acids. Their [Ru(bipy)2]2+ complexes [(bipy)2Ru(BocLysCH2py)]2+ (4), [(bipy)2Ru(AcCysCH2py)]2+ (5), and [(bipy)2Ru(AcCys(CH2)2py)]2+ (6) have been prepared by reactions of the ligands with [Ru(bipy)2Cl2]. On the basis of 1H-NMR spectroscopy, 4−6 can be described best as trans-tetrapyridine complexes with the lysine amino N atom and the cysteine S atom occupying one of the apical positions. It was shown by luminescence spectroscopy that 4 can serve as a possible photoredox-active module for the construction of photochemically active peptides. The redox properties of the complexes are described with the aid of the Lever parameters. It was demonstrated that the amino acid ligands in 5 and 6 can be viewed as methionine units. Particularly interesting is the unique redox chemistry of 4. Upon metal oxidation, a two-electron ligand oxidation occurred, followed by fast hydrolytic cleavage of the lysine−methylpyridine N−C bond. The physical and chemical properties of the compounds are discussed in terms of future applications in biomimetic chemistry such as the activation of small molecules.
Nonexcitatory Stimulus Delivery Improves Left Ventricular Function in Hearts with Left Bundle Branch Block (2002)

Our preliminary data demonstrate that NES is superior to BV pacing in improving LV function in an animal model with LBBB. Moreover, we demonstrated that NES does not affect transmitral valve flow and subsequently LV diastolic function.
Delineating the pharmacophoric elements of huperzine A: importance of the unsaturated three-carbon bridge to its AChE inhibitory activity (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDelineating the pharmacophoric elements of huperzine A: importance of the unsaturated three-carbon bridge to its AChE inhibitory activityAlan P. Kozikowski, Chris P. Miller, Fumio Yamada, Yuan Ping Pang, Jacqueline H. Miller, Michael McKinney, and Richard G. BallCite this: J. Med. Chem. 1991, 34, 12, 3399–3402Publication Date (Print):December 1, 1991Publication History Published online1 May 2002Published inissue 1 December 1991https://pubs.acs.org/doi/10.1021/jm00116a010https://doi.org/10.1021/jm00116a010research-articleACS PublicationsRequest reuse permissionsArticle Views332Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Sonographic monitoring of hepatic cryosurgery in an experimental animal model (1985)

Several cryosurgical techniques for freezing hepatic parenchyma have been developed. To assess the efficacy of sonographic monitoring of hepatic cryosurgery, an experimental animal model was devised. Real-time sonography was used to monitor the in vivo evolution of hypothermically induced hepatic cryolesions in four mongrel dogs. The results suggest that sonography is an effective and accurate means of monitoring the entire freezing and thawing cycle in hepatic cryosurgery.
Structure and expression of the nuclear gene coding for the chloroplast ribosomal protein L21: developmental regulation of a housekeeping gene by alternative promoters. (1993)

We have cloned and sequenced the nuclear gene of the chloroplast ribosomal protein L21 (rpl21) of Spinacia oleracea. The gene consists of five exons and four introns. All introns are located in the sequence which corresponds to the Escherichia coli-like central core of the protein. L21 mRNA is present in photosynthetic (leaves) and nonphotosynthetic (roots and seeds) plant organs, although large quantitative differences exist. Primer extension and S1 nuclease mapping experiments revealed the existence of two types of transcripts in leaves. The two corresponding start sites were defined as P1 and P2. In roots and seeds, we found only the shorter of the two transcripts (initiated at P2). The nucleotide sequence surrounding P2 resembles promoters for housekeeping and vertebrate r-protein genes. Analysis of several promoter constructions by transient expression confirmed that both transcripts originate from transcription initiation. Results are interpreted to mean that the expression of the rpl21 gene is regulated by alternative promoters. One of the promoters (P2) is constitutive, and the other one (P1) is specifically induced in leaves, i.e., its activation should be related to the transformation of amyloplasts or proplastids to chloroplasts. The gene thus represents the first example of a housekeeping gene which is regulated by the organ-specific usage of alternative promoters. Primer extension analysis and S1 nuclease mapping of another nucleus-encoded chloroplast ribosomal protein gene (rps1) give evidence that the same type of regulation by two-promoter usage might be a more general phenomenon of plant chloroplast-related ribosomal protein genes. Preliminary results indicate that presence of conserved sequences within the rpl21 and rps1 promoter regions which compete for the same DNA binding activities.
802.11ac: A Survival Guide (2013)

The next frontier for wireless LANs is 802.11ac, a standard that increases throughput beyond one gigabit per second. This concise guide provides in-depth information to help you plan for 802.11ac, with technical details on design, network operations, deployment, and monitoring. Author Matthew Gastan industry expert who led the development of 802.11-2012 and security task groups at the Wi-Fi Allianceexplains how 802.11ac will not only increase the speed of your network, but its capacity as well. Whether you need to serve more clients with your current level of throughput, or serve your existing client load with higher throughput, 802.11ac is the solution. This book gets you started.Understand how the 802.11ac protocol works to improve the speed and capacity of a wireless LAN Explore how beamforming increases speed capacity by improving link margin, and lays the foundation for multi-user MIMO Learn how multi-user MIMO increases capacity by enabling an AP to send data to multiple clients simultaneously Plan when and how to upgrade your network to 802.11ac by evaluating client devices, applications, and network connections
Automated System Monitoring and Notification With Swatch (1993)

This paper describes an approach to monitoring events on a large number of servers and workstations. While modern UNIX systems are capable of logging a variety of information concerning the health and status of their hardware and operating system software, they are generally not configured to do so. Even when this information is logged, it is often hidden in places that are either not monitored regularly or are susceptible to deletion or modification by a successful intruder. Also, a system administrator must often monitor several, perhaps dozens, of systems. To address these problems, our approach begins with the modification of certain system programs to enhance their logging capabilities. In addition, our approach calls for the logging facilities on each of these systems to be configured in such a way as to send a copy of the critical system and security related information to a dependable, secure, central logging host system. As one might expect, this central log can see a megabyte...
The distribution of repeating [Gal beta 1,4GlcNAc beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines BW5147 and PHAR 2.1. (1984)

The occurrence and distribution of the repeating disaccharide [Gal beta 1,4GlcNAc beta 1,3] in the different types of Asn-linked oligosaccharides in mouse lymphoma BW5147 cells have been studied. Glycopeptides were prepared from cells grown in medium containing [6-3H]galactose, and the bi-, tri-, and tetraantennary Asn-linked oligosaccharides were fractionated by serial lectin affinity chromatography on concanavalin A-Sepharose, pea lectin -Sepharose, leukoagglutinating phytohemagglutinin-agarose, and Datura stramonium agglutinin-agarose. As described in this report, the latter lectin binds glycopeptides that contain either the repeating N-acetyllactosamine sequence or an outer mannose residue substituted at C-2 and C-6 by N-acetyllactosamine. The isolated glycopeptides were subjected to methylation analysis, specific exoglycosidase treatments, and digestion with Escherichia freundii endo-beta-galactosidase. Our data indicate that approximately two-thirds of the tetraantennary and one-half of the triantennary Asn-linked oligosaccharides contain repeating N-acetyllactosamine sequences in at least one branch. Many of the repeating sequences contain an additional galactose residue linked alpha 1,3 to a penultimate galactose residue. By contrast, less than 10% of the biantennary oligosaccharides contain the repeating disaccharide. The distribution of the repeating N-acetyllactosamine unit was also examined in a cell line ( PHAR 2.1) that is deficient in UDP-GlcNAc:alpha-mannoside beta 1,6-N-acetylglucosaminyltransferase. These cells are unable to synthesize tetraantennary and certain triantennary species and instead accumulate biantennary oligosaccharides. The total content of repeating N-acetyllactosamine units is greatly decreased in this line, and those that are present are found predominantly in triantennary Asn-linked oligosaccharides. These results demonstrate that the repeating N-acetyllactosamine sequence occurs commonly in complex-type Asn-linked oligosaccharides in BW5147 cells but is confined primarily to tri- and teraantennary species.
Toward Metabolic Phenomics: Analysis of Genomic Data Using Flux Balances (1999)

Small genome sequencing and annotations are leading to the definition of metabolic genotypes in an increasing number of organisms. Proteomics is beginning to give insights into the use of the metabolic genotype under given growth conditions. These data sets give the basis for systemically studying the genotype-phenotype relationship. Methods of systems science need to be employed to analyze, interpret, and predict this complex relationship. These endeavors will lead to the development of a new field, tentatively named phenomics. This article illustrates how the metabolic characteristics of annotated small genomes can be analyzed using flux balance analysis (FBA). A general algorithm for the formulation of in silico metabolic genotypes is described. Illustrative analyses of the in silico Escherichia coli K-12 metabolic genotypes are used to show how FBA can be used to study the capabilities of this strain.
Low–interfacial toughness materials for effective large-scale deicing (2019)

Easy ice removal The accumulation of ice on a surface can lead to hazardous conditions, such as on the surface of an airplane wing or the side of a tall building. Ice adhesion, even to a surface treated to minimize the bonded force, will usually depend on the amount of surface coverage. Golovin et al. compared strength-limited deicing with toughness-limited deicing. Whereas normal deicing materials focus on minimizing the adhesion strength, the authors show that if a material is designed with low-adhesion toughness, deicing is no longer a function of the coverage area. Science , this issue p. 371
Physical principles for scalable neural recording (2013)

Simultaneously measuring the activities of all neurons in a mammalian brain at millisecond resolution is a challenge beyond the limits of existing techniques in neuroscience. Entirely new approaches may be required, motivating an analysis of the fundamental physical constraints on the problem. We outline the physical principles governing brain activity mapping using optical, electrical, magnetic resonance, and molecular modalities of neural recording. Focusing on the mouse brain, we analyze the scalability of each method, concentrating on the limitations imposed by spatiotemporal resolution, energy dissipation, and volume displacement. Based on this analysis, all existing approaches require orders of magnitude improvement in key parameters. Electrical recording is limited by the low multiplexing capacity of electrodes and their lack of intrinsic spatial resolution, optical methods are constrained by the scattering of visible light in brain tissue, magnetic resonance is hindered by the diffusion and relaxation timescales of water protons, and the implementation of molecular recording is complicated by the stochastic kinetics of enzymes. Understanding the physical limits of brain activity mapping may provide insight into opportunities for novel solutions. For example, unconventional methods for delivering electrodes may enable unprecedented numbers of recording sites, embedded optical devices could allow optical detectors to be placed within a few scattering lengths of the measured neurons, and new classes of molecularly engineered sensors might obviate cumbersome hardware architectures. We also study the physics of powering and communicating with microscale devices embedded in brain tissue and find that, while radio-frequency electromagnetic data transmission suffers from a severe power-bandwidth tradeoff, communication via infrared light or ultrasound may allow high data rates due to the possibility of spatial multiplexing. The use of embedded local recording and wireless data transmission would only be viable, however, given major improvements to the power efficiency of microelectronic devices.
Central Nervous System Delivery of Recombinant Ciliary Neurotrophic Factor by Polymer Encapsulated Differentiated C 2 C 12 Myoblasts (1996)

Neurotrophic factors hold promise for the treatment of neurodegenerative diseases. Intrathecal transplantation of polymer encapsulated cell lines genetically engineered to release neurotrophic factors provides a means to deliver them continuously behind the blood-brain barrier. Long-term delivery, however, may benefit from the use of conditionally mitotic cells to avoid the overgrowth observed with continuously dividing cell lines. Myoblast lines have all the advantages of dividing cell lines, i.e., unlimited availability, possibility for in vitro screening for the presence of pathogens, suitability for stable gene transfer and clonal selection. Furthermore they can be differentiated into a nonmitotic stage upon exposure to low-serum-containing medium. In this study, mouse C2C12 myoblasts were transfected with a pNUT expression vector containing the human ciliary neurotrophic factor (CNTF) gene. hCNTF expression and bioactivity were demonstrated by Northern blot, ELISA assay, and measurement of choline acetyltransferase (ChAT) activity in embryonic spinal cord motor neuron cultures. One C2C12 clone was found to secrete 200 ng of CNTF/10(6) cells per day. The rate of secretion of hCNTF was not altered upon differentiation of C2C12 myoblasts. A bromodeoxyuridine (BrdU) proliferation assay indicated that approximately 12% of the myoblasts continue to divide after 4 days in low-serum-containing medium. The presence of the herpes simplex thymidine kinase gene (HSV-tk) in the expression vector, however, provides a way to eliminate these dividing myoblasts upon exposure to ganciclovir, therefore increasing the safety of the encapsulation technology using established cell lines. Encapsulated hCNTF-C2C12 cells can partially rescue motor neurons from axotomy-induced cell death. In adult rats, intrathecal implantation of encapsulated hCNTF-C2C12 cells or control C2C12 confirmed the long-term survival of these cells and their potential use as a source of neurotophic factors for the treatment of neurodegenerative diseases.
Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy (2009)

Analysis of the multilineage differentiation capacity of SCD parent clones and daughter clones enabled us to formulate a new hierarchical schema for MSC self-renewal and differentiation in which a self-renewing multipotent MSC gives rise to more restricted self-renewing progenitors that gradually lose differentiation potential until a state of complete restriction to the fibroblast is reached.
Recent Progress in Nucleic Acid Aptamer-Based Biosensors and Bioassays (2008)

As the key constituents of the genetic code, the importance of nucleic acids to life has long been appreciated. Despite being composed of only four structurally similar nucleotides, single-stranded nucleic acids, as in single-stranded DNAs and RNAs, can fold into distinct three-dimensional shapes due to specific intramolecular interactions and carry out functions beyond serving as templates for protein synthesis. These functional nucleic acids (FNAs) can catalyze chemical reactions, regulate gene expression, and recognize target molecules. Aptamers, whose name is derived from the Latin word aptus meaning "to fit", are oligonucleotides that can bind their target ligands with high affinity and specificity. Since aptamers exist in nature but can also be artificially isolated from pools of random nucleic acids through a process called in vitro selection, they can potentially bind a diverse array of compounds. In this review, we will discuss the research that is being done to develop aptamers against various biomolecules, the progress in engineering biosensors by coupling aptamers to signal transducers, and the prospect of employing these sensors for a range of chemical and biological applications. Advances in aptamer technology emphasizes that nucleic acids are not only the fundamental molecules of life, they can also serve as research tools to enhance our understanding of life. The possibility of using aptamer-based tools in drug discovery and the identification of infectious agents can ultimately augment our quality of life.
Development of a safe oral Aβ vaccine using recombinant adeno-associated virus vector for Alzheimer's disease (2004)

A new oral vaccine for Alzheimer's disease was developed using recombinant adeno-associated virus vector carrying Abeta cDNA (AAV/Abeta). Oral administration of the vaccine without adjuvant induced the expression and secretion of Abeta1-43 or Abeta1-21 in the epithelial cell layer of the intestine in amyloid precursor protein transgenic mice. Serum antibody levels were elevated for more than six months, while T cell proliferative responses to Abeta was not detected. Brain Abeta burden was significantly decreased compared to the control without inflammatory changes. This oral AAV/Abeta vaccine seems to be promising for prevention and treatment of Alzheimer's disease.
The synthesis and properties of a calixarene-based ‘sugar bowl’ (1995)

P. Linnane, T. D. James and S. Shinkai, J. Chem. Soc., Chem. Commun., 1995, 1997 DOI: 10.1039/C39950001997
Malignant transformation by overproduction of translation initiation factor eIF4G. (1997)

N4G3, a cell line that overexpresses translation initiation factor eIF4G, one of the components of eIF4F, was made by stable transfection of the human eIF4G cDNA into NIH3T3 cells. The cells expressed 80-100 times greater levels of eIF4G mRNA than did NIH3T3 cells. N4G3 cells formed transformed foci on a monolayer of cells, showed anchorage-independent growth, and formed tumors in nude mice. These results indicate that overexpression of eIF4G caused malignant transformation of NIH3T3 cells. It is also known that overexpression of eIF4E, another component of eIF4F, causes transformation of NIH3T3 cells. However, there was no difference in the amount of eIF4E protein between N4G3 and NIH3T3 cells, indicating that cell transformation does not involve a change in eIF4E levels. The results may be due to an effect of eIF4G on translational control of protein synthesis directed by mRNAs having long 5'-untranslated region.