gebhart/patrepeval huggingface dataset:
Biochemical characterization of peptide alpha-amidation enzyme activities of human neuroendocrine lung cancer cell lines. (1993)

Peptide alpha-amidation is a posttranslational modification of approximately half of all endocrine and neuroendocrine peptide hormones, including several hormones with mitogenic effects for tumor cells, and is typically essential for complete hormonal bioactivity. alpha-Amidated peptide hormones have been reported to be autocrine growth factors for small cell lung cancer cells. We report here that a variety of human lung tumor cell lines express both enzymes required for the two-step conversion of inactive glycine-extended peptides into their active COOH-terminal alpha-amide analogues. Human tumor cell peptidylglycine alpha-amidation enzymes are present in multiple molecular forms. Both proteins are metalloenzymes which are present at highest concentrations in secretory granules in neuroendocrine cell lines. The expression of these enzymes is positively correlated with expression of other markers of the neuroendocrine phenotype, such as DOPA decarboxylase. Peptidylglycine alpha-amidating enzyme-specific activities are approximately 50-fold higher in extracts of endocrine cell lines (lung small cell and carcinoid) than of nonendocrine lines. Biochemical characterization of the peptidylglycine alpha-amidating enzymes will enable development of tools for detection of endocrine processes in the early stages of neoplasia and for interruption of autocrine stimulation pathways in tumor cells.
Broadband high frequency differential coupler (2002)

This paper describes the design methodology of a broadband, high frequency differential coupler. The output signal of this differential coupler is proportional to the difference of 2 input ports, i.e. proportional to the differential signal of the 2 inputs. This signal is made using a hybrid structure. The overall performance of this differential coupler and the hybrid structure is characterized by the differential coupling factor and by the common mode rejection ratio. A pair of broadband impedances are used to scale the 2 incoming signals with 2 complex coefficients. A symmetric structure of two identical broadband directional couplers and a symmetric power combiner are used afterwards to obtain the differential signal. The high bandwidth of both the terminations, the directional couplers and the power combiner make it possible to obtain a differential coupling factor and a common mode rejection ratio which is frequency insensitive over several decades. Measurements demonstrate a differential coupler with a bandwidth of more than 3 decades.
Compositional pointer and escape analysis for Java programs (1999)

This paper presents a combined pointer and escape analysis algorithm for Java programs. The algorithm is based on the abstraction of points-to escape graphs, which characterize how local variables and fields in objects refer to other objects. Each points-to escape graph also contains escape information, which characterizes how objects allocated in one region of the program can escape to be accessed by another region. The algorithm is designed to analyze arbitrary regions of complete or incomplete programs, obtaining complete information for objects that do not escape the analyzed regions.
Genetic elimination of dengue vector mosquitoes (2011)

An approach based on mosquitoes carrying a conditional dominant lethal gene (release of insects carrying a dominant lethal, RIDL) is being developed to control the transmission of dengue viruses by vector population suppression. A transgenic strain, designated OX3604C, of the major dengue vector, Aedes aegypti, was engineered to have a repressible female-specific flightless phenotype. This strain circumvents the need for radiation-induced sterilization, allows genetic sexing resulting in male-only releases, and permits the release of eggs instead of adult mosquitoes. OX3604C males introduced weekly into large laboratory cages containing stable target mosquito populations at initial ratios of 8.5-101 OX3604Ctarget eliminated the populations within 10-20 weeks. These data support the further testing of this strain in contained or confined field trials to evaluate mating competitiveness and environmental and other effects. Successful completion of the field trials should facilitate incorporation of this approach into area-wide dengue control or elimination efforts as a component of an integrated vector management strategy.
VCO FQR millimeter-wave phase-locked sources (1989)

A high quality mm-wave source is absolutely necessary in various mm-nave engineering systems. It determines, to we extent, the upper performance limit of the system. It CM be said that a phase-locked source ( PIS ) is the only choice which possesses both very high long-term stability and good near-end and far-end phase noise characteristics. So a PLS is the key device in many mm-wave systems.
Grammar-based whitebox fuzzing (2008)

Whitebox fuzzing is a form of automatic dynamic test generation, based on symbolic execution and constraint solving, designed for security testing of large applications. Unfortunately, the current effectiveness of whitebox fuzzing is limited when testing applications with highly-structured inputs, such as compilers and interpreters. These applications process their inputs in stages, such as lexing, parsing and evaluation. Due to the enormous number of control paths in early processing stages, whitebox fuzzing rarely reaches parts of the application beyond those first stages.
An Elisa for PGE2Utilizing Monoclonal Antibody (1988)

An ELISA for PGE2 has been developed which is sensitive to concentrations of 0.5 to 20.0 ng PGE2/ml. Mouse monoclonal anti-PGE2 ascites is utilized in a binding competition between the test sample and an adsorbed conjugate of PGE2-BSA. The antibody which remains bound to the solid phase is quantitated colorimetrically by incubation with alkaline phosphatase-conjugated goat anti-mouse IgG followed by incubation with p-nitro-phenylphosphate. PGE1, PGA1, PGA2, PGB2, 6-keto-PGF1 alpha, PGF2 alpha, 13,14-dihydro-15-keto-PGE2, thromboxane B2 and arachidonic acid showed minimal cross-reactivity with the anti-PGE2. The PGE2 ELISA permits the quantitative analysis of large numbers of samples at a fraction of the cost and time required to process a commercial RIA kit. When linked to the appropriate computer software, data collection and analysis can be performed in less than 10 minutes per 96-well plate. Furthermore, the use of an ELISA system eliminates the radioactive and toxic chemical waste generated by RIA methods.
Effect of 18 months of treatment with alfacalcidol on bone in patients with mild to moderate chronic renal failure (2004)

Long-term treatment with alfacalcidol is safe and might be beneficial for the preservation of bone mass in the pre-dialysis stages of CRF, most likely through a reduction in bone turnover as estimated from the changes of the biochemical bone markers.
A randomized phase II study of concurrent docetaxel plus vaccine versus vaccine alone in metastatic androgen-independent prostate cancer. (2006)

This is the first clinical trial to show that docetaxel can be administered safely with immunotherapy without inhibiting vaccine specific T-cell responses. Furthermore, patients previously vaccinated with an anticancer vaccine may respond longer to docetaxel compared with a historical control of patients receiving docetaxel alone. Larger prospective clinical studies will be required to validate these findings.
Tau‐tubulin kinase 1 (TTBK1), a neuron‐specific tau kinase candidate, is involved in tau phosphorylation and aggregation (2006)

Neurofibrillary tangles, which are major pathological hallmarks of Alzheimer's disease (AD), are composed of paired helical filaments (PHFs) containing hyperphosphorylated tau. Specific kinases regulate tau phosphorylation and are closely linked to the pathogenesis of AD. We have characterized a human tau-tubulin kinase 1 (TTBK1) gene located on chromosome 6p21.1. TTBK1 is a serine/threonine/tyrosine kinase that is conserved among species and belongs to the casein kinase 1 superfamily. It is specifically expressed in the brain, especially in the cytoplasm of cortical and hippocampal neurons. TTBK1 phosphorylates tau proteins in both a Mg2+- and a Mn2+-dependent manner. Phosphopeptide mapping and immunoblotting analysis confirmed a direct tau phosphorylation by TTBK1 at Ser198, Ser199, Ser202 and Ser422, which are also phosphorylated in PHFs. TTBK1 also induces tau aggregation in human neuronal cells in a dose-dependent manner. We conclude that TTBK1 is a neuron-specific dual kinase involved in tau phosphorylation at AD-related sites and is also associated with tau aggregation.
Growth factor regulation of the novel class II phosphoinositide 3-kinases (2001)

It is well established that the class-I phosphoinositide (PI) 3-kinases play a crucial role in growth factor signalling pathways. However, evidence has recently emerged that the alpha isoform of the class-II PI 3-kinase (PI 3K-C2alpha) is activated by growth factors, although the consequences of this are poorly understood. Here we demonstrate that the activation of PI 3K-C2alpha is not associated with a change in subcellular localization. Furthermore, we provide the first evidence that PI 3K-C2beta is activated by insulin, albeit with slower kinetics than activation of PI 3K-C2alpha. These findings suggest that both these class-II PI 3-kinase isoforms are likely to participate in insulin-signalling pathways in the cell.
Topological quantum computation based on chiral Majorana fermions (2018)

The chiral Majorana fermion is a massless self-conjugate fermion which can arise as the edge state of certain 2D topological matters. It has been theoretically predicted and experimentally observed in a hybrid device of a quantum anomalous Hall insulator and a conventional superconductor. Its closely related cousin, the Majorana zero mode in the bulk of the corresponding topological matter, is known to be applicable in topological quantum computations. Here we show that the propagation of chiral Majorana fermions leads to the same unitary transformation as that in the braiding of Majorana zero modes and propose a platform to perform quantum computation with chiral Majorana fermions. A Corbino ring junction of the hybrid device can use quantum coherent chiral Majorana fermions to implement the Hadamard gate and the phase gate, and the junction conductance yields a natural readout for the qubit state.
Cardiac Stem Cells Regenerate the Infarcted Heart (2005)

Extended abstract of a paper presented at Microscopy and Microanalysis 2005 in Honolulu, Hawaii, USA, July 31--August 4, 2005
Authentication in the Taos operating system (1993)

We describe a design and implementation of security for a distributed system. In our system, applications access security services through a narrow interface. This interface provides a notion of identity that includes simple principals, groups, roles, and delegations. A new operating system component manages principals, credentials, and secure channels. It checks credentials according to the formal rules of a logic of authentication. Our implementation is efficient enough to support a substantial user community.
Systematic characterization of mutations in yeast acetohydroxyacid synthase (2003)

Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyses the first step in branched-chain amino acid biosynthesis and is the target for sulfonylurea and imidazolinone herbicides, which act as potent and specific inhibitors. Mutants of the enzyme have been identified that are resistant to particular herbicides. However, the selectivity of these mutants towards various sulfonylureas and imidazolinones has not been determined systematically. Now that the structure of the yeast enzyme is known, both in the absence and presence of a bound herbicide, a detailed understanding of the molecular interactions between the enzyme and its inhibitors becomes possible. Here we construct 10 active mutants of yeast AHAS, purify the enzymes and determine their sensitivity to six sulfonylureas and three imidazolinones. An additional three active mutants were constructed with a view to increasing imidazolinone sensitivity. These three variants were purified and tested for their sensitivity to the imidazolinones only. Substantial differences are observed in the sensitivity of the 13 mutants to the various inhibitors and these differences are interpreted in terms of the structure of the herbicide-binding site on the enzyme.
History of Erythropoiesis-Stimulating Agents, the Development of Biosimilars, and the Future of Anemia Treatment in Nephrology (2017)

Biosimilars, or biologic drugs that are designed to be highly similar to approved reference biologic drugs, have been available in Europe for more than 10 years with no unusual or unexpected effects compared to their reference biologics whose patents have expired. Given the success of the biosimilar approval pathway pioneered in Europe, it has served as a global reference for other regulatory authorities to establish and implement biosimilar licensure frameworks, including the United States (US), the largest pharmaceutical market in the world. Given 10 of the top 25 drugs sold in 2014 were biologics, and considering the rising costs of healthcare, biosimilars have the potential to become a significant part of the US market. Key Messages: For the nephrology community, the recent patent expiries for epoetin alfa (Epogen®, Amgen and Procrit®, Johnson &amp; Johnson) have created the opportunity to develop biosimilar EPOs. And while no biosimilar in this therapeutic class is approved in the US, there are proposed biosimilars in development.
Up-regulation of vaults may be necessary but not sufficient for multidrug resistance (2001)

Vaults are ribonucleoprotein complexes comprised of the 100 kDa major vault protein (MVP), the 2 high m.w. vault proteins p193 (VPARP) and p240 (TEP1) and an untranslated small RNA (vRNA). Increased levels of MVP, vault-associated vRNA and vaults have been linked directly to non-P-glycoprotein-mediated multidrug resistance (MDR). To further characterize the putative role of vaults in MDR, expression levels of all of the vault proteins were examined in various MDR cell lines. Subcellular fractionation of vault particles revealed that all 3 vault proteins are increased in MDR cells compared to the parental, drug-sensitive cells. Furthermore, protein analysis of subcellular fractions of the drug-sensitive, MVP-transfected AC16 cancer cell line indicated that vault levels are increased, in this stable line. Since TEP1 is shared by both vaults and the telomerase complex, TEP1 protein (and vault) levels were compared with telomerase activity in a variety of cell lines, including various MDR lines. Our studies demonstrate that while vault levels may be a good predictor of drug resistance, their up-regulation alone is not sufficient to confer the drug-resistant phenotype. This implies a requirement of an additional factor(s) for vault-mediated MDR.
Adaptive Forward Error Control for Digital Satellite Systems (1985)

Two hybrid schemes of time-frequency resource sharing to increase the rain margin of Ku-and Ka-band satellite systems are proposed. Scheme 1 requires sharing a small pool of bandwidth for adaptive forward error control coding, sharing a small pool of time frame for rate reduction, and sharing a portion of low frequency time-division multiple access (TDMA) back-up frame for downlink transmission to the rain affected stations. Scheme 2 utilizes variable rate modulation and forward error correction, shares a small pool of time frame for rate reduction, and shares a portion of low frequency TDMA back-up frame. Effective usable capacities of the system using these schemes are calculated. Distribution of resources in order to maximize the effective usable capacity is also analyzed. The results obtained are compared with other adaptive schemes. Preliminary analysis shows that the utilized capacity of scheme 1 exceeds 99 percent of the effective usable capacity possible if it never rains for an outage of 0.05 percent and fade margin of 2.5 dB. For scheme 2 similar performance is achievable at a fade margin of 1.5 dB. For higher outage objectives the loss of effective utilized capacity is higher for scheme 2.
Swelling and Elastic Properties of Polyelectrolyte Gels (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSwelling and Elastic Properties of Polyelectrolyte GelsR. Skouri, F. Schosseler, J. P. Munch, and S. J. CandauCite this: Macromolecules 1995, 28, 1, 197–210Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ma00105a026https://doi.org/10.1021/ma00105a026research-articleACS PublicationsRequest reuse permissionsArticle Views1688Altmetric-Citations183LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
NO-induced migraine attack: strong increase in plasma calcitonin gene-related peptide (CGRP) concentration and negative correlation with platelet serotonin release (2003)

The aim of the present study was to investigate changes in the plasma calcitonin gene-related peptide (CGRP) concentration and platelet serotonin (5-hydroxytriptamine, 5-HT) content during the immediate headache and the delayed genuine migraine attack provoked by nitroglycerin. Fifteen female migraineurs (without aura) and eight controls participated in the study. Sublingual nitroglycerin (0.5 mg) was administered. Blood was collected from the antecubital vein four times: 60 min before and after the nitroglycerin application, and 60 and 120 min after the beginning of the migraine attack (mean 344 and 404 min; 12 subjects). In those subjects who had no migraine attack (11 subjects) a similar time schedule was used. Plasma CGRP concentration increased significantly (P<0.01) during the migraine attack and returned to baseline after the cessation of the migraine. In addition, both change and peak, showed significant positive correlations with migraine headache intensity (P<0.001). However, plasma CGRP concentrations failed to change during immediate headache and in the subjects with no migraine attack. Basal CGRP concentration was significantly higher and platelet 5-HT content tended to be lower in subjects who experienced a migraine attack. Platelet serotonin content decreased significantly (P<0.01) after nitroglycerin in subjects with no migraine attack but no consistent change was observed in patients with migraine attack. In conclusion, the fact that plasma CGRP concentration correlates with the timing and severity of a migraine headache suggests a direct relationship between CGRP and migraine. In contrast, serotonin release from platelets does not provoke migraine, it may even counteract the headache and the concomitant CGRP release in this model.
Thermo-Mechanical Reliability of Nano-Silver Sintered Joints versus Lead-Free Solder Joints for Attaching Large-Area Silicon Devices (2010)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;Nano-silver sintered bonding was carried out at 275°C and under 3MPa pressures, and soldering in a vacuum reflowing oven to reduce voiding. Both joints are subject to large shear stresses due to the mismatch in coefficients of thermal expansion (CTE) between the chip and the substrate. In this study, residual stresses in the chip-on-substrate assemblies were determined by measuring the bending curvatures of the bonded structures. An in-house optical setup measured the bending curvatures using a thin-film stress measurement technique. From the measured bending curvatures and the mechanical properties of the constituent materials, residual stresses were calculated. The thermo-mechanical reliabilities of both joining techniques were tested by thermal cycling. The chip assemblies were cycled between -40°C and 125°C (100 minutes of cycle time, 10 minutes of dwell time) and the changes in their bending curvatures were measured. Residual stresses were found to decrease with temperature cycling, which may be due to stress relaxation from creep or crack formation in the die-attach materials. Failure analysis of sintered nano-silver joints, eutectic Sn-3.5Ag solder (SAC305) joints and eutectic SnCu0.7Ni solder (SN100C) joints was performed by means of cross-sectioning and scanning electron microscopy (SEM) after 800 thermal cycles. For both solder materials with copper substrate, fatigue fracture started at the edge of the solder, and crack propagation across the solder joint was observed. No cracks were found at the joints between silicon and sintered nano-silver. The stress-relaxation behavior of all die-attach layers are hypothesized.&lt;/div&gt;&lt;/div&gt;
Structural refinement of protein A mimetic peptide (2011)

A novel dendrimeric peptide ligand dubbed D-PAM-Φ was designed to achieve a high capacity for human IgG through the decoration of the D-PAM scaffold. The design criteria based on the introduction of small hydrophobic groups on the D-PAM structure were supported by the recently published solid-state structure of D-PAM complexed to the Fc fragment of a recombinant human IgG1 and by molecular dynamic simulations that provided information on the mode of binding of phenylacetyl-D-PAM (D-PAM-Φ). D-PAM-Φ was immobilised on an activated solid support and compared with the parent D-PAM affinity matrix. The newly obtained affinity sorbent was evaluated for its capacity to selectively capture polyclonal human IgG; the binding capacity was approximately 10 mg/ml, an almost 10-fold enhancement with respect to the D-PAM-functionalised matrices without the specificity of binding being reduced. The new ligand was also effective in the capturing of recombinant humanised IgG1 from a clarified cell culture supernatant. Under a typical laboratory-scale affinity chromatography assembly and preliminarily optimised binding conditions, the affinity purification of humanised IgG1 from culture supernatants rendered the desired product, with purity higher than 90%. The results suggest that the application of the computational approach on the structure of the D-PAM-Fc complex may be very valuable in the development of novel lead molecules for the downstream processing of human or humanised antibodies used in therapy.