gebhart/patrepeval huggingface dataset:
Concurrent Prediction of Hospital Mortality and Length of Stay from Risk Factors on Admission (2002)

Piecewise exponential models may be useful in predicting LOS, especially if determinants of mortality are separated from determinants of discharge alive.
Instant radiosity (1997)

We present a fundamental procedure for instant rendering from the radiance equation. Operating directly on the textured scene description, the very efficient and simple algorithm produces photorealistic images without any finite element kernel or solution discretization of the underlying integral equation. Rendering rates of a few seconds are obtained by exploiting graphics hardware, the deterministic technique of the quasi-random walk for the solution of the global illumination problem, and the new method of jittered low discrepancy sampling.
Oncology : a multidisciplinary textbook (1995)

Part 1 Cancer epidemiology and screening The cell biology of cancer The molecular biology of cancer Cancer immunology Principles of cancer pathology Imaging techniques PET scanning in oncology Principles of surgical oncology Principles of radiation therapy Principles of medical oncology Evaluation of new drugs in cancer therapy Principles of cytokine therapy Infection diseases in cancer Psychological aspects of cancer Principles of pallivative care Cancer therapy and fertility. Part 2 Hodgkin's disease Non-Hodgkin's lymphoma Acute leukaemias Chronic leukaemias Myeloma Cancer of the cervix and corpus Ovarian tumours Gestational trophoblastic tumours Sarcomas Bone tumours Melanomas and skin cancers Central nervous system tumours Neuroendocrine tumours Paediatric oncology Testicular cancer Bladder cancer Prostate cancer Hypernephroma Early breast cancer Advanced breast cancer Surgical aspects of head and neck cancer Non-surgical aspects of head and neck cancer Thyroid cancer Small cell lung cancer Surgical aspects of GI tract cancer GI tract non-surgical treatment Hepato-biliary cancer Pancreatic cancer.
Evaluation of a New Capillary Electrochromatography/Mass Spectrometry Interface Using Short Columns and High Field Strengths for Rapid and Efficient Analyses (1996)

A prototype combined capillary electrochromatography/electrospray mass spectrometry interface (CEC/ESI-MS) has been designed, built and evaluated for rapid, electrically-driven chromatographic analyses which facilitates high separation efficiencies with mass spectrometric detection. The system has an integral electrokinetic injection system and separation is performed in the interface probe using short columns with high field strengths. The system is equally capable of performing on-line capillary electrophoresis mass spectrometry (CE/MS)
Cell-Mediated Immune Proliferative Responses to HIV-1 of Chimpanzees Vaccinated with Different Vaccinia Recombinant Viruses (1989)

The only animal that can be reproducibly infected with HIV, and that thus provides an experimental system for testing the effectiveness of prototype vaccines, is the chimpanzee. We compared proliferative responses to HIV and to vaccinia virus (VV) antigens of lymphocytes taken at various times from chimpanzees vaccinated with recombinant VV expressing different HIV genes. Animals were immunized with the original VV strain, as control, or with constructs expressing gp160 (VV160) given exclusively or in combination with one or two other constructs producing p25 (VV25), F/3'-orf (VVF), or the human interleukin-2 (IL-2) gene, which was included in an attempt to amplify immune responses. Irrespective of the HIV gene utilized, lymphocyte proliferation to HIV was usually weak and rapidly decreased after each inoculation, contrasting with strong and sustained responses to VV. Lack of adequate recall reactivity after challenge with fixed autologous lymphocytes expressing VV-produced HIV antigens indicated that vaccination resulted only in low levels of HIV-specific memory cell priming. The use of IL-2-producing VV did not lead to increased responsiveness. Reactivity to soluble purified gp160, but not to p25, could be detected in PBL from animals that had received both VV160 and VV25, while immunization with VVF resulted in a significant response to this protein in one of two animals. The transient nature of T cell reactivity to HIV might explain why, in similar studies, chimpanzees were not protected from infection with live HIV.
Genetic determination of essential residues of the Vibrio cholerae actin cross‐linking domain reveals functional similarity with glutamine synthetases (2009)

Actin cross-linking domains (ACDs) are distinct domains found in several bacterial toxins, including the Vibrio cholerae MARTX toxin. The ACD of V. cholerae (ACD(Vc)) catalyses the formation of an irreversible iso-peptide bond between lysine 50 and glutamic acid 270 on two actin molecules in an ATP- and Mg/Mn(2+)-dependent manner. In vivo, cross-linking depletes the cellular pool of G-actin leading to actin cytoskeleton depolymerization. While the actin cross-linking reaction performed by these effector domains has been significantly characterized, the ACD(Vc) catalytic site has remained elusive due to lack of significant homology to known proteins. Using multiple genetic approaches, we have identified regions and amino acids of ACD(Vc) required for full actin cross-linking activity. Then, using these functional data and structural homology predictions, it was determined that several residues demonstrated to be important for ACD(Vc) activity are conserved with active-site residues of the glutamine synthetase family of enzymes. Thus, the ACDs are a family of bacterial toxin effectors that may be evolutionarily related to ligases involved in amino acid biosynthesis.
Nucleotide and predicted peptide sequence of feline interferon—gamma (IFN-γ) (1995)

Interferon gamma is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. We now report the cloning and sequencing of feline interferon gamma cDNA which was generated by the polymerase chain reaction. At the nucleotide level feline ifn-gamma shares 78% and 63% homology with human and murine cDNA equivalents. At the amino acid level the feline IFN-gamma shares 63% and 43% homology with human and murine homologs respectively.
Structure-Function Analysis of Arg-Gly-Asp Helix Motifs in αvβ6 Integrin Ligands (2007)

Data relating to the structural basis of ligand recognition by integrins are limited. Here we describe the physical requirements for high affinity binding of ligands to alpha v beta6. By combining a series of structural analyses with functional testing, we show that 20-mer peptide ligands, derived from high affinity ligands of alpha v beta6 (foot-and-mouth-disease virus, latency associated peptide), have a common structure comprising an Arg-Gly-Asp motif at the tip of a hairpin turn followed immediately by a C-terminal helix. This arrangement allows two conserved Leu/Ile residues at Asp(+1) and Asp(+4) to be presented on the outside face of the helix enabling a potential hydrophobic interaction with the alpha v beta6 integrin, in addition to the Arg-Gly-Asp interaction. The extent of the helix determines peptide affinity for alpha v beta6 and potency as an alpha v beta6 antagonist. A major role of this C-terminal helix is likely to be the correct positioning of the Asp(+1) and Asp(+4) residues. These data suggest an explanation for several biological functions of alpha v beta6 and provide a structural platform for design of alpha v beta6 antagonists.
Conjugation of methotrexate to poly (L-lysine) as a potential way to overcome drug resistance (1980)

Methotrexate (MTX) was conjugated through a carbodiimide-catalyzed reaction to poly (L-lysines) of various molecular sizes at a ratio of approximately one molecule per 27 lysyl residues. These conjugates were tested on cultured, Chinese hamster, ovary cells known to be drug resistant because of a deficient methotrexate transport. The cellular uptake of conjugated drug far exceeded the uptake of free drug in both drug sensitive and resistant lines. The conjugated drug inhibited the growth of the transport-deficient cells at concentrations at which free drug had no effect. The conjugate failed to inhibit dihydrofolate reductase in vitro. This and other evidence indicate that the strong pharmacologic effect of the MTX-poly(Lys) conjugate is due to the intracellular--presumably intralysosomal--hydrolysis of its polymeric backbone followed by the release inside the cell of an active form of MTX. This conclusion is supported by data obtained with conjugates using poly(D-lysine) as a carrier. This optical isomer is not susceptible to common proteolytic enzymes and MTX-poly(D-Lys) has not growth inhibitory effect whatever on either transport proficient or deficient CHO-cells. MTX-poly(L-lys) can thus be seen as a lysosome-activated drug.
Oxidative refolding of recombinant prochymosin (1999)

The disulphide-coupled refolding of recombinant prochymosin from Escherichia coli inclusion bodies was investigated. Prochymosin solubilized from inclusion bodies is endowed with free thiol groups and disulphide bonds. This partially reduced form undergoes renaturation more efficiently than the fully reduced form, suggesting that some native structural elements existing in inclusion bodies and remaining after denaturation function as nuclei to initiate correct refolding. This assumption is supported by the finding that in the solubilized prochymosin molecule the cysteine residues located in the N-terminal domain of the protein are not incorrectly paired with the other cysteines in the C-terminal domain. Addition of GSH/GSSG into the refolding system facilitates disulphide rearrangement and thus enhances renaturation, especially for the fully reduced prochymosin. Based on the results described in this and previous papers [Tang, Zhang and Yang (1994) Biochem. J. 301, 17-20], a model to depict the refolding process of prochymosin is proposed. Briefly, the refolding process of prochymosin consists of two stages: the formation and rearrangement of disulphide bonds occurs at the first stage in a pH 11 buffer, whereas the formation and adjustment of tertiary structure leading to the native conformation takes place at the second stage at pH 8. The pH 11 conditions help polypeptides to refold in such a way as to favour the formation of native disulphide bonds. Disulphide rearrangement, the rate-limiting step during refolding, can be achieved by thiol/disulphide exchange initiated by free thiol groups present in the prochymosin polypeptide, GSH/GSSG or protein disulphide isomerase.
Chemotherapy-Induced Acral Erythema (1994)

Figure 1. Chemotherapy-Induced Acral Erythema. Acral cyanosis can be seen in a 61-year-old woman with non-Hodgkin's lymphoma. The lesions developed while the patient had severe neutropenia during a course of therapy consisting of methotrexate with leucovorin rescue, doxorubicin, cyclophosphamide, vincristine, prednisone, and bleomycin. The disorder is characterized by intense, painful erythema of the palms and soles, often culminating in the formation of vesicles or bullae. Chemotherapeutic agents are one of the known causes.
Dopamine Transporter: Biochemistry, Pharmacology and Imaging (1990)

In recent years, there has been substantial progress in studying the dopamine transporter, a unique component of the functioning dopaminergic nerve terminal. The transporter has been studied by direct binding techniques using a variety of ligands which function as inhibitors of transport. Analogues of these ligands have been used as photoaffinity labels to solubilize and further characterize the transporter. While a variety of drugs bind to the transporter, it is clear that the transporter may serve as an important drug receptor, particularly for the reinforcing properties of some psychostimulants such as cocaine. An extension of the in vitro ligand-binding studies reveals that it is possible to preferentially label the transporter in vivo. The success of in vivo labeling has lead to successful positron emission tomographic scanning studies of the transporter. These studies in turn have revealed the usefulness of imaging the transporter, a measure of the presence of dopaminergic nerve terminals, as a potential diagnostic tool in Parkinson's disease.
Delivering QOS in XML Data Stream Processing Using Load Shedding (2012)

In recent years, we have witnessed the emergence of new types of systems that deal with large volumes of streaming data. Examples include financial data analysis on feeds of stock tickers, sensorbased environmental monitoring, network track monitoring and click stream analysis to push customized advertisements or intrusion detection. Traditional database management systems (DBMS), which are very good at managing large volumes of stored data, are not suitable for this, as streaming needs low-latency processing on live data from push-based sources. Data Stream Management Systems (DSMS) are fast emerging to address this new type of data, but faces challenging issues, such as unpredictable data arrival rate. On bursty mode, processing need surpasses available system capacity affecting the Quality of Service (QoS) adversely. The system overloading is even more acute in XML data streams compared to relational streams due to its extra resource requirements for data preparation and result construction. The main focus of this paper is to find out suitable ways to process this high volume of data streams dealing with the spikes in data arrival gracefully, under limited or fixed system resources in the XML stream context.
How to avoid unwanted email (1998)

article Free Access Share on How to avoid unwanted email Author: Robert J. Hall AT&T Labs Research, Florham Park, N.J. AT&T Labs Research, Florham Park, N.J.View Profile Authors Info & Claims Communications of the ACMVolume 41Issue 3March 1998 pp 88–95https://doi.org/10.1145/272287.272329Published:01 March 1998Publication History 38citation1,484DownloadsMetricsTotal Citations38Total Downloads1,484Last 12 Months36Last 6 weeks11 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Bone Mineral Density in Women with Depression (1996)

Past or current depression in women is associated with decreased bone mineral density.
A comprehensive proteome map of growing Bacillus subtilis cells (2004)

The proteome of growing cells of Bacillus subtilis was analyzed in order to provide the basis for its application in microbial physiology. DNA arrays were used to calculate the number of genes transcribed in growing cells. From the 4100 B. subtilis genes, 2515 were actively transcribed in cells grown under standard conditions. From these genes 1544 proteins should be covered by our standard gel system pI 4-7. Using this standard gel system and supplementary zoom gels (pI 5.5-6.7, 5-6, 4.5-5.5, and 4-5) 693 proteins which are expressed in growing cells were detected that cover more than 40% of the vegetative proteome predicted for this region. Particularly broad coverage and thus comprehensive monitoring will be possible for central carbohydrate metabolism (glycolysis, pentose phosphate shunt, and citric acid cycle), amino acid synthesis pathways, purine and pyrimidine metabolism, fatty acid metabolism, and main cellular functions like replication, transcription, translation, and cell wall synthesis. Comparing the theoretical pI and Mr values with those experimentally determined a reasonable correlation was found for the majority of protein spots. By a color code outliers with dramatic deviations in charge or mass were visualized that may indicate post-translational modifications. In addition to the cytosolic neutral and alkaline proteins, 130 membrane proteins were found relying on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separation in combination with electrospray ionization-tandem mass spectrometry (ESI-MS/MS) techniques. The vegetative proteome containing 876 proteins in total is now ready for physiological applications. Two main proteome fractions (pI 4-7 and zoom gel pI 4.5-5.5) should be sufficient for such high-throughput physiological proteomics.
Effects of protozoa on bacterial nitrogen recycling in the rumen. (2000)

The effects of protozoa on ruminal NH3-N kinetics and bacterial N recycling were measured in five sheep (57.6+/-7.1 kg BW, x +/- SD) with ruminal and duodenal cannulas in naturally faunated, defaunated, and refaunated periods. The sheep were fed a diet of 239 g of alfalfa haylage and 814 g of barley concentrate per day (DM basis) divided into 12 equal portions and allocated at 2-h intervals. A pulse dose of 300 mg of 15N as [15N]NH4Cl was administered into the rumen (on d 1 and 15) and 300 mg of 15N as [15N]urea was administered intravenously to the blood (d 8). Enrichment of 15N was measured in ruminal NH3-N, bacterial N, and plasma urea N over a period of 35 h. Total collection of urine was made for 5 d and analyzed for purine derivatives to calculate the flow of microbial N. Ruminal parameters and nutrient digestibilities were also measured. Sheep were defaunated using a rumen washing procedure 50 d prior to measurements in the defaunated period. Sheep were refaunated with ruminal contents from a faunated sheep receiving the same diet. Measurements began 26 d following refaunation, at which time protozoal numbers had returned to those in the originally faunated sheep. Data reported in parentheses are for faunated, defaunated, and refaunated sheep, respectively. Total culturable and cellulolytic bacterial numbers were unaffected by defaunation, but there was an increase in flow of microbial N from the rumen (10.8, 17.3, and 11.1 g N/d; P < .05) in the defaunated period. Flux, irreversible loss, and intraruminal recycling of NH3-N and recycling of NH3-N from plasma urea N were not affected by defaunation. Defaunation had no effect on reducing the absolute amount (13.8, 10.0, and 11.3 g N/d; P > .20) of bacterial N recycling and the percentage of N flux through the bacterial N pool. Total-tract digestion was reduced in defaunated compared with faunated sheep by 8, 17, 15, and 32% for OM, N, NDF, and ADF, respectively. In conclusion, defaunation improved ruminal N metabolism through the enhancement of bacterial protein synthesis, and improvement in the flow of microbial protein to the host animal.
Keynote speakers: Wireless power transfer: From long-distance transmission to short-range charging (2013)

Since Nikola Tesla proposed the Wardenclyfee Tower in 1889 for the wireless power transmission to date, it has been over a century. The idea was later picked up and implemented continuously in the last half a century. Recently in 2007, MIT research group picked up the old concept and implemented for potential applications into telecommunications. This talk will address briefly the state of-the-art research and development progresses in the wireless power transmission technology and also potential applications. The talk is categorized into two aspects, that is, (a) the long-distance and high-power wireless energy transmission and (b) the short-range and low-power wireless energy transmission. For the long-distance high-power transmission, the talk will cover atmospheric effects, high-power microwave generation, transmitting antennas, EMC/EMI effects on radio frequency wireless system, rectennas, and biological effects on human being and animals. For the short-range wireless power transmission, the review will touch inductive couple effects and their applications onto power-grid systems, wireless power charging systems, mine-tunnel power charge system, and medical applications. The talk will, of course, discuss on advantages and short-comings of these proposals.
ABL1 Methylation Is a Distinct Molecular Event Associated With Clonal Evolution of Chronic Myeloid Leukemia (1999)

Methylation of the proximal promoter of the ABL1 oncogene is a common epigenetic alteration associated with clinical progression of chronic myeloid leukemia (CML). In this study we queried whether both the Ph'-associated and normal ABL1 alleles undergo methylation; what may be the proportion of hematopoietic progenitors bearing methylated ABL1 promoters in chronic versus acute phase disease; whether methylation affects the promoter uniformly or in patches with discrete clinical relevance; and, finally, whether methylation of ABL1 reflects a generalized process or is gene-specific. To address these issues, we adapted the techniques of methylation-specific PCR and bisulfite-sequencing to study the regulatory regions of ABL1 and other genes with a role in DNA repair or genotoxic stress response. In cell lines established from CML blast crisis, which only carry a single ABL1 allele nested within the BCR-ABL fusion gene, ABL1 promoters were universally methylated. By contrast, in clinical samples from patients at advanced stages of disease, both methylated and unmethylated promoter alleles were detectable. To distinguish between allele-specific methylation and a mixed cell population pattern, we studied the methylation status of ABL1 in colonies derived from single hematopoietic progenitors. Our results showed that both methylated and unmethylated promoter alleles coexisted in the same colony. Furthermore, ABL1 methylation was noted in the vast majority of colonies from blast crisis, but not chronic-phase CML. Both cell lines and clinical samples from acute-phase CML showed nearly uniform hypermethylation along the promoter region. Finally, we showed that ABL1 methylation does not reflect a generalized process and may be unique among DNA repair/genotoxic stress response genes. Our data suggest that specific methylation of the Ph'-associated ABL1 allele accompanies clonal evolution in CML.
Leech-Derived Thrombin Inhibitors: From Structures to Mechanisms to Clinical Applications (2010)

ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTLeech-Derived Thrombin Inhibitors: From Structures to Mechanisms to Clinical ApplicationsMaría Ángeles Corral-Rodríguez†, Sandra Macedo-Ribeiro‡, Pedro José Barbosa Pereira‡, and Pablo Fuentes-Prior*†View Author Information† Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Sant Antoni Maria Claret 167, 08025 Barcelona, Spain‡ IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal*To whom correspondence should be addressed. Phone: 034 93 291 9045. Fax: 034 93 455 2331. E-mail: [email protected]Cite this: J. Med. Chem. 2010, 53, 10, 3847–3861Publication Date (Web):February 15, 2010Publication History Received24 November 2009Published online15 February 2010Published inissue 27 May 2010https://pubs.acs.org/doi/10.1021/jm901743xhttps://doi.org/10.1021/jm901743xreview-articleACS PublicationsCopyright © 2010 American Chemical SocietyRequest reuse permissionsArticle Views2208Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Genetics,Inhibition,Inhibitors,Monomers,Peptides and proteins Get e-Alerts