gebhart/patrepeval huggingface dataset:
Research Instruments for Measuring the Impact of School Libraries on Student Achievement and Motivation (2001)

A two-year study of public school library programs was conducted in one of the largest and most diverse states in the USA-New York. This three-phase study extends previous statewide library impact studies by using multiple research methods with multiple stakeholders to investigate the school library's impact on:(1) student achievement, (2) motivation for learning, and (3) technology use, as well as a range of other variables (e.g., principal-librarian relationship, librarian-teacher collaboration, library services and resources for students with disabilities). This article describes the design, development, testing and validation of online survey instruments used in the first two phases of this research. The article concludes with a number of recommendations for ways in which these instruments might be used by school library professionals to assess the impact of their programs and services on students in their schools and districts.
A seamless trespass: germ cell migration across the seminiferous epithelium during spermatogenesis (2007)

During spermatogenesis, preleptotene spermatocytes traverse the blood-testis barrier (BTB) in the seminiferous epithelium, which is reminiscent of viral pathogens breaking through the tight junctions of host epithelial cells. The process also closely resembles the migration of leukocytes across endothelial tight junctions to reach inflammation sites. Cell adhesion molecules of the immunoglobulin superfamily (e.g., JAM/CAR/nectin) participate in germ cell migration by conferring transient adhesion between Sertoli and germ cells through homophilic and heterophilic interactions. The same molecules also comprise the junctional complexes at the BTB. Interestingly, JAM/CAR/nectin molecules mediate virus uptake and leukocyte transmigration in strikingly similar manners. It is likely that the strategy used by viruses and leukocytes to break through junctional barriers is used by germ cells to open up the inter-Sertoli cell junctions. In associating these diverse cellular events, we highlight the "guiding" role of JAM/CAR/nectin molecules for germ cell passage. Knowledge on viral invasion and leukocyte transmigration has also shed insights into germ cell movement during spermatogenesis.
Navigation system based on ceiling landmark recognition for autonomous mobile robot (2002)

In this paper, we propose a vision based navigation system for autonomous mobile robots which recognizes air-conditioning device (anemo) as landmarks located on ceiling. Because landmarks on ceiling are not obstructed by any objects, robots can find them easier than landmarks on the floor. In the recognition process, gathering quadrangles in the image are regarded as anemo, the gravity center and the slope of these quadrangles are calculated. Then the navigation system can calculate the distance and the angle of the anemo from map information. As a result, the navigation system can identify the present robot's position and orientation. By the proposed algorithm, if the landmark is not being taken completely, the landmark is inferred by partial information. In the experiments, we compared the error made by the robot which are navigated by our system with the error made by the robot which moves based on deadreckoning.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A 6 &#215; 6 inch 20 lines-per-inch liquid-crystal display panel (1973)

An integrated 14 000 picture element 36-in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> flat screen display panel has been constructed by a combination of thin-film transistor and nematic liquid-crystal technology. The design, fabrication, and present performance of the panel is discussed. The design of the peripheral circuits, which permit the presentation of low resolution off-the-air TV pictures on the panel is described in another paper.
The Diagnosis and Work-up of the Patient With Gastroparesis (2000)

Gastroparesis-or delayed gastric emptying--is associated with upper gastrointestinal symptoms that include early satiety, nausea, vomiting, regurgitation, fullness, and bloating. Gastroparesis should be considered in the diagnosis of a patient with these symptoms after mechanical and structural lesions have been ruled out. This review briefly summarizes gastric motor physiology and discusses the etiology and diagnostic approach to the treatment of a patient with possible gastroparesis. We highlight the methods available to measure gastric motility and describe their relative advantages and disadvantages.
Branched peptide‐amphiphiles as self‐assembling coatings for tissue engineering scaffolds (2006)

An important challenge in regenerative medicine is the design of suitable bioactive scaffold materials that can act as artificial extracellular matrices. We reported previously on a family of peptide-amphiphile (PA) molecules that self-assemble into high-aspect ratio nanofibers under physiological conditions, and can display bioactive peptide epitopes along each nanofiber's periphery. One type of PA displays its epitope at a branched site using a lysine dendron, a molecular feature that improves epitope availability on the nanofiber surface. In this work, we describe the application of these branched PA (b-PA) systems as self-assembling coatings for fiber-bonded poly(glycolic acid) scaffolds. b-PAs bearing variations of the RGDS adhesion epitope from fibronectin were shown by elemental analysis to coat repeatably onto fiber scaffolds. The retention of supramolecular organization after coating on the scaffold was demonstrated through spectroscopic identification of beta-sheet structures and the close association of hydrophobic tails in a model pyrene-containing PA system. Primary human bladder smooth muscle cells demonstrated greater initial adhesion to b-PA-functionalized scaffolds than to bare scaffolds or to those coated with linear PAs. This strategy of molecular design and coating may have potential application in bladder tissue regeneration.
Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase (1991)

A recombinant DNA polymerase derived from the thermophilic eubacterium Thermus thermophilus (Tth pol) was found to possess very efficient reverse transcriptase (RT) activity in the presence of MnCl2. Many of the problems typically associated with the high degree of secondary structure present in RNA are minimized by using a thermostable DNA polymerase for reverse transcription, and predominantly full-length products can be obtained. The cDNA can also be amplified in the polymerase chain reaction (PCR) with the same enzyme. The Tth pol was observed to be greater than 100-fold more efficient in a coupled RT/PCR than the analogous DNA polymerase from Thermus aquaticus (Taq pol). The sensitivity of the reactions performed by Tth pol allowed for the detection of ethidium bromide stained products starting with as little as 100 copies of synthetic cRNA. Similar results were also obtained with RNA from a Philadelphia-chromosome positive cell line. Detection of IL-1 alpha mRNA was possible starting with 80 pg of total cellular RNA. The ability of Tth pol to perform both reverse transcription and DNA amplification will undoubtedly prove useful in the detection, quantitation, and cloning of cellular and viral RNA.
Complete regression of villous adenomas of the colon using piroxicam, a nonsteroidal anti-inflammatory drug (1996)

A 73-year old patient with a past history of rectal carcinoma was found to have two sessile villous adenomas of the cecum. Because he refused surgical resection, a course of nonsteroidal anti-inflammatory drug therapy using piroxicam was given, and the villous adenomas regressed within three months. The cecum has remained clear at one year.
Identification of errors introduced during high throughput sequencing of the T cell receptor repertoire (2011)

Caution is needed in interpreting repertoire data due to potential contamination with mis-sequence reads. However, a high association of errors with phred score, high relatedness of erroneous sequences with the parental sequence, dominance of specific nt substitutions, and skewed ratio of forward to reverse reads among erroneous sequences indicate approaches to filter erroneous sequences from repertoire data sets.
Tumor-derived exosomes modulate PD-L1 expression in monocytes (2017)

In chronic lymphocytic leukemia (CLL), monocytes and macrophages are skewed toward protumorigenic phenotypes, including the release of tumor-supportive cytokines and the expression of immunosuppressive molecules such as programmed cell death 1 ligand 1 (PD-L1). To understand the mechanism driving protumorigenic skewing in CLL, we evaluated the role of tumor cell-derived exosomes in the cross-talk with monocytes. We carried out RNA sequencing and proteome analyses of CLL-derived exosomes and identified noncoding Y RNA hY4 as a highly abundant RNA species that is enriched in exosomes from plasma of CLL patients compared with healthy donor samples. Transfer of CLL-derived exosomes or hY4 alone to monocytes resulted in key CLL-associated phenotypes, including the release of cytokines, such as C-C motif chemokine ligand 2 (CCL2), CCL4, and interleukin-6, and the expression of PD-L1. These responses were abolished in Toll-like receptor 7 (TLR7)-deficient monocytes, suggesting exosomal hY4 as a driver of TLR7 signaling. Pharmacologic inhibition of endosomal TLR signaling resulted in a substantially reduced activation of monocytes in vitro and attenuated CLL development in vivo. Our results indicate that exosome-mediated transfer of noncoding RNAs to monocytes contributes to cancer-related inflammation and concurrent immune escape via PD-L1 expression.
Novel Psychoactive Phenethylamines: Impact on Genetic Material (2020)

Psychedelic and stimulating phenethylamines belong to the family of new psychoactive substances (NPS). The acute toxicity framework has begun to be investigated, while studies showing genotoxic potential are very limited or not available. Therefore, in order to fill this gap, the aim of the present work was to evaluate the genotoxicity by treating TK6 cells with 2C-H, 2C-I, 2C-B, 25B-NBOMe, and the popular 3,4-Methylenedioxymethylamphetamine (MDMA). On the basis of cytotoxicity and cytostasis results, we selected the concentrations (6.25-35 µM) to be used in genotoxicity analysis. We used the micronucleus (MN) as indicator of genetic damage and analyzed the MNi frequency fold increase by an automated flow cytometric protocol. All substances, except MDMA, resulted genotoxic; therefore, we evaluated reactive oxygen species (ROS) induction as a possible mechanism at the basis of the demonstrated genotoxicity. The obtained results showed a statistically significant increase in ROS levels for all genotoxic phenethylamines confirming this hypothesis. Our results highlight the importance of genotoxicity evaluation for a complete assessment of the risk associated also with NPS exposure. Indeed, the subjects who do not have hazardous behaviors or require hospitalization by using active but still "safe" doses could run into genotoxicity and in the well-known long-term effects associated.
A Novel Cancer Therapy by Skin Delivery of Indoleamine 2,3-Dioxygenase siRNA (2009)

Antitumor immunity can be effectively elicited by physical delivery of siRNAs targeting immunoregulatory genes in skin dendritic cells in vivo, as shown by IDO and IDO2 in this report.
Parallel Gradient Distribution in Unconstrained Optimization (1995)

A parallel version is proposed for a fundamental theorem of serial unconstrained optimization. The parallel theorem allows each of k parallel processors to use simultaneously a different algorithm, such as a descent, Newton, quasi-Newton, or conjugate gradient algorithm. Each processor can perform one or many steps of a serial algorithm on a portion of the gradient of the objective function assigned to it, independently of the other processors. Eventually a synchronization step is performed which, for differentiable convex functions, consists of taking a strong convex combination of the k points found by the k processors. A more general synchronization step, applicable to convex as well as nonconvex functions, consists of taking the best point found by the k processors or any point that is better. The fundamental result that we establish is that any accumulation point of the parallel algorithm is stationary for the nonconvex case and is a global solution for the convex case. Computational testing on the Thinking Machines CM-5 multiprocessor indicates a speedup of the order of the number of processors employed.
Facile Synthesis of Substituted 2-Iminohydantoins (1987)

Abstract A practical method for the preparation of substituted 2-iminohydantoins by cyclization of N-alkoxycarbonyl-α-amino acyl cyanamides is described.
From para-dimethoxybenzene toward crown benzenophanes: 1,3,10,14-tetraoxa[3.5]paracyclophane (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFrom para-dimethoxybenzene toward crown benzenophanes: 1,3,10,14-tetraoxa[3.5]paracyclophaneHenning Hopf, Ralf Utermoehlen, Peter G. Jones, Jean Pierre Desvergne, and Henri Bouas-LaurentCite this: J. Org. Chem. 1992, 57, 20, 5509–5517Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September 1992https://pubs.acs.org/doi/10.1021/jo00046a038https://doi.org/10.1021/jo00046a038research-articleACS PublicationsRequest reuse permissionsArticle Views175Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis (2002)

The availability of sequences of entire genomes has dramatically increased the number of protein targets, many of which will need to be overexpressed in cells other than the original source of DNA. Gene synthesis often provides a fast and economically efficient approach. The synthetic gene can be optimized for expression and constructed for easy mutational manipulation without regard to the parent genome. Yet design and construction of synthetic genes, especially those coding for large proteins, can be a slow, difficult and confusing process. We have written a computer program that automates the design of oligonucleotides for gene synthesis. Our program requires simple input information, i.e. amino acid sequence of the target protein and melting temperature (needed for the gene assembly) of synthetic oligonucleotides. The program outputs a series of oligonucleotide sequences with codons optimized for expression in an organism of choice. Those oligonucleotides are characterized by highly homogeneous melting temperatures and a minimized tendency for hairpin formation. With the help of this program and a two-step PCR method, we have successfully constructed numerous synthetic genes, ranging from 139 to 1042 bp. The approach presented here simplifies the production of proteins from a wide variety of organisms for genomics-based studies.
Hierarchical Network between the Components of the Multi-tRNA Synthetase Complex (2006)

The macromolecular tRNA synthetase complex consists of nine different enzymes and three non-enzymatic factors. This complex was recently shown to be a novel signalosome, since many of its components are involved in signaling pathways in addition to their catalytic roles in protein synthesis. The structural organization and dynamic relationships of the components of the complex are not well understood. Here we performed a systematic depletion analysis to determine the effects of structural intimacy and the turnover of the components. The results showed that the stability of some components depended on their neighbors. Lysyl-tRNA synthetase was most independent of other components for its stability whereas it was most required for the stability of other components. Arginyl- and methionyl-tRNA synthetases had the opposite characteristics. Thus, the systematic depletion of the components revealed the functional reason for the complex formation and the assembly pattern of these multi-functional enzymes and their associated factors.
The Path of Carbon in Photosynthesis. XXV. Plant and Algal Growth Responses to Glycopyranosides (2009)

Substituted glycopyranosides were applied to various plant species as foliar and root treatments, with and without nutrient supplements, and growth was compared to untreated or nutrient controls. When formulated with specific fertilizers, particularly available nitrogen, methyl and ethyl glycopyranosides significantly enhanced the overall growth of vascular plants compared to controls provided with the same nutrients. In contrast, vascular plants treated with alkylglycopyranosides without nutrients showed growth equivalent to that of untreated controls. Nitrogen-supplemented alkylglycopyranosides provided as foliar applications of 100 mM, or as root treatments between 35 and 50 mM, consistently enhanced root and shoot productivity. In addition to the vascular plant species tested, the brown alga Laminaria saccharina exhibited significant growth enhancement over nutrient controls when treated with alkylglycopyranosides supplemented with nutrients, especially under low irradiance. Overall, these results indicate that growth of photosynthetic organisms is enhanced by exogenous alkylglycopyranosides supplemented with nitrogen and micronutrients, but not by alkylglycopyranosides alone.
Human chorionic gonadotropin (hCG) as inhibitior of E-selectin-mediated cell adhesion. (2005)

hCG is an effective selectin antagonist. This fact suggests that hCG may play a role in preventing leukocyte adhesion on the foetal syncytiotrophoblast or the metastatic activity of trophoblastic tumour cells.
Direct Allorecognition Promotes Activation of Bystander Dendritic Cells and Licenses Them for Th1 Priming: A Functional Link Between Direct and Indirect Allosensitization (2005)

T-cell sensitization to indirectly presented alloantigens (indirect pathway of allorecognition) plays a critical role in chronic rejection. The usual very efficient priming of such self-restricted, T helper type 1 (Th1)-deviated CD4+ T cells obviously conflicts with the fact that allogeneic MHC molecules are poorly immunogenic per se. The aim of the present study is to elucidate whether direct allosensitization induces production of inflammatory mediators that may affect recruitment and activation of immature bystander (host) dendritic cells (DC). These potential mechanisms were studied in vitro by conducting primary allogeneic mixed leucocyte reactions (MLR), mimicking the priming phase in secondary lymphoid organs, and secondary MLR, mimicking the effector phase within the graft. Primary, and particularly secondary, MLR supernatants were found to contain high levels of monocyte/immature DC-recruiting CC chemokines and pro-inflammatory cytokines. Exposure of immature DC to primary or secondary MLR supernatants was found to upregulate CD40 expression and further enhanced lipopolysaccharide-induced interleukin-12 (IL-12) p70 production. Secondary MLR supernatants additionally induced upregulation of CD86 and deviated allogeneic T-cell responses towards Th1 (enhanced interferon-gamma production without concomitant induction of detectable IL-4 or IL-10 production). These findings indicate that direct allorecognition may act as a Th1-deviating adjuvant for indirect allosensitization.
Alopecia: possible causes and treatments, particularly in captive nonhuman primates. (2009)

Alopecia (hair loss) occurs in some nonhuman primates housed in captivity and is of concern to colony managers and veterinarians. Here we review the characteristics, potential causes, and treatments for this condition. Although we focus on nonhuman primates, relevant research on other mammalian species is discussed also, due to the relative paucity of studies on alopecia in the primate literature. We first discuss the cycle of hair growth and explain how this cycle can be disrupted to produce alopecia. Numerous factors may be related to hair loss and range from naturally occurring processes (for example, seasonality, aging) to various biologic dysfunctions, including vitamin and mineral imbalances, endocrine disorders, immunologic diseases, and genetic mutations. We also address bacterial and fungal infections, infestation by parasites, and atopic dermatitis as possible causes of alopecia. Finally, we examine the role of psychogenic factors, such as stress. Depending on the presumed cause of the hair loss, various treatment strategies can be pursued. Alopecia in nonhuman primates is a multifaceted disorder with many potential sources. For this reason, appropriate testing for various disease conditions should be completed before alopecia is considered to be related to stress.
Cloning of Δ12‐ and Δ6‐desaturases from Mortierella alpina and recombinant production of γ‐linolenic acid in Saccharomyces cerevisiae (1999)

Two cDNA clones with homology to known desaturase genes were isolated from the fungus Mortierella alpina. The open reading frame in one clone encoded 399 amino acids and exhibited delta12-desaturase activity when expressed in Saccharomyces cerevisiae in the presence of endogenous fatty acid substrate oleic acid. The insert in another clone contained an open reading frame encoding 457 amino acids and exhibited delta6-desaturase activity in S. cerevisiae in the presence of exogenous fatty acid substrate linoleic acid. Expression of the delta12-desaturase gene under appropriate media and temperature conditions led to the production of linoleic acid at levels up to 25% of the total fatty acids in yeast. When linoleic acid was provided as an exogenous substrate to the yeast cultures expressing the delta6-desaturase activity, the level of gamma-linolenic acid reached 10% of the total yeast fatty acids. Co-expression of both the delta6- and delta12-desaturase cDNA resulted in the endogenous production of gamma-linolenic acid. The yields of gamma-linolenic acid reached as high as 8% of total fatty acids in yeast.
Principles of physical biochemistry (1998)

Preface. I. MACROMOLECULAR STRUCTURE AND DYNAMICS. 1. Biological Macromolecules. 2. Thermodynamic Principles. 3. Molecular Thermodynamics. 4. Statistical Mechanics. 5. Methods for the Separation and Characterization of Macromolecules. 6. X-Ray Diffraction. 7. Scattering from Solutions of Macromolecules. II. SPECTROSCOPY 8. Quantum Mechanics and Spectroscopy. 9. Absorption Spectroscopy. 10. Linear and Circular Dichroism. 11. Emission Spectroscopy. 12. Nuclear Magnetic Resonance Spectroscopy. III. SOLUTION BEHAVIOR OF MACROMOLECULES. 13. Macromolecules in Solution: Thermodynamics and Equilibria. 14. Thermodynamics of Transport Processes. 15. Chemical Equilibria Involving Macromolecules. Solutions to Odd-Numbered Exercises. Index.