gebhart/patrepeval huggingface dataset:
Motion-Resistant Remote Imaging Photoplethysmography Based on the Optical Properties of Skin (2014)

Remote imaging photoplethysmography (RIPPG) can achieve contactless monitoring of human vital signs. However, the robustness to a subject's motion is a challenging problem for RIPPG, especially in facial video-based RIPPG. The RIPPG signal originates from the radiant intensity variation of human skin with pulses of blood and motions can modulate the radiant intensity of the skin. Based on the optical properties of human skin, we build an optical RIPPG signal model in which the origins of the RIPPG signal and motion artifacts can be clearly described. The region of interest (ROI) of the skin is regarded as a Lambertian radiator and the effect of ROI tracking is analyzed from the perspective of radiometry. By considering a digital color camera as a simple spectrometer, we propose an adaptive color difference operation between the green and red channels to reduce motion artifacts. Based on the spectral characteristics of photoplethysmography signals, we propose an adaptive bandpass filter to remove residual motion artifacts of RIPPG. We also combine ROI selection on the subject's cheeks with speeded-up robust features points tracking to improve the RIPPG signal quality. Experimental results show that the proposed RIPPG can obtain greatly improved performance in accessing heart rates in moving subjects, compared with the state-of-the-art facial video-based RIPPG methods.
Recent developments in first-principles force fields for molecules in nanoporous materials (2013)

There is a great need to identify high-performance nanoporous materials for gas storage, separation and catalysis to solve a wide range of industrially relevant challenges. Molecular simulations have become an important complement to experiments in the screening and selection of suitable porous materials. Successful implementation of molecular simulations requires accurate force fields (FFs) to describe the interactions of guest molecules with porous frameworks. However, developing accurate and transferable FFs for porous materials can be extremely challenging. While generic FFs and experimentally-derived FFs work well for many simple systems, they often fail to describe interactions in more complex porous materials. Since first-principles quantum mechanical (QM) approaches are capable of accurately predicting intermolecular interactions, deriving FFs from QM data without experimental input is a promising solution. In this paper, we review recent developments in deriving first-principles FFs for molecules in a variety of nanoporous materials. We summarize and classify the methodologies used in these studies, and discuss the factors that are responsible for the accuracy and reliability of FFs. Finally, we conclude the review with a discussion of current challenges and future directions for this rapidly growing field.
The metabolism of cinnamic acid by healthy and phenylketonuric adults: A kinetic study (1984)

The enzyme phenylalanine ammonia lyase taken orally has been found to reduce the rise in blood phenylalanine that normally occurs following a protein meal. Therefore the enzyme has a potential use in the management of the genetic disease phenylketonuria. The enzyme mediates the conversion of phenylalanine to cinnamic acid and its possible clinical future has necessitated a more detailed study of the product of its reaction. Cinnamic acid is a compound of low toxicity which is converted in the mammalian body primarily to hippuric acid. We have examined the kinetics of this process in a healthy male and in two patients with untreated phenylketonuria. In addition we have attempted to clarify the inconsistencies in earlier published work about the status of other, minor metabolites. Following an oral load of sodium (2H6) cinnamate there is an increase in urinary hippuric acid largely due to the excretion of (2H5) hippuric acid. In the subjects studied there was no major difference in the rate of elimination although the amount of cinnamic acid converted was less in those with phenylketonuria. This may reflect reduced first-pass absorption by the liver in untreated phenylketonuria enabling increased uptake to occur in other parts of the body.
Purification and identification of 91‐kDa neutrophil gelatinase (1991)

Human neutrophils were found to release a 91-kDa gelatinase that is serologically related to tumor-derived gelatinolytic enzymes, as evidenced by immunoprecipitation. In order to identify the neutrophil gelatinase, the activity in conditioned medium from human neutrophil suspensions was purified by affinity chromatography on a gelatin substrate. The 91-kDa active enzyme was further separated from other stainable protein bands by classical SDS PAGE and blotting to a solid support. Amino-terminal sequence analysis of blotted proteins showed that the 91-kDa enzyme is a truncated form of tumor-derived 92-kDa gelatinase (type IV collagenase), lacking eight residues at the NH2-terminus. Sequence analysis of enzymatically inactive cleavage products of this neutrophil gelatinase demonstrated that the gelatin-binding part of the molecule is restricted to the amino-terminal third. Exocytosis of gelatinase-containing granules from neutrophils occurred spontaneously within 6 h after neutrophil plating. When the cells were triggered with the phorbol ester phorbol 12-myristate 13-acetate, a strong secretagogue, rapid gelatinase release was observed. When granulocytes were stimulated with the neutrophil-activating peptide interleukin-8, maximal exocytosis occurred within 1 h. The almost immediate release of neutrophil gelatinase after stimulation of the cells with a chemotactic factor might play a key role in remodeling of the extracellular matrix during granulocyte movement in response to chemotactic stimuli.
Light-induced Proton Release of Phytochrome Is Coupled to the Transient Deprotonation of the Tetrapyrrole Chromophore (2005)

The Pr --> Pfr phototransformation of the bacteriophytochrome Agp1 from Agrobacterium tumefaciens and the structures of the biliverdin chromophore in the parent states and the cryogenically trapped intermediate Meta-R(C) were investigated with resonance Raman spectroscopy and flash photolysis. Strong similarities with the resonance Raman spectra of plant phytochrome A indicate that in Agp1 the methine bridge isomerization state of the chromophore is ZZZasa in Pr and ZZEssa in Pfr, with all pyrrole nitrogens being protonated. Photoexcitation of Pr is followed by (at least) three thermal relaxation components in the formation of Pfr with time constants of 230 micros and 3.1 and 260 ms. H2O/D2O exchange reveals kinetic isotope effects of 1.9, 2.6, and 1.3 for the respective transitions that are accompanied by changes of the amplitudes. The second and the third relaxation correspond to the formation and decay of Meta-R(C), respectively. Resonance Raman measurements of Meta-R(C) indicate that the chromophore adopts a deprotonated ZZE configuration. Measurements with a pH indicator dye show that formation and decay of Meta-R(C) are associated with proton release and uptake, respectively. The stoichiometry of the proton release corresponds to one proton per photoconverted molecule. The coupling of transient chromophore deprotonation and proton release, which is likely to be an essential element in the Pr --> Pfr photocon-version mechanism of phytochromes in general, may play a crucial role for the structural changes in the final step of the Pfr formation that switch between the active and the inactive state of the photoreceptor.
Mass-defect filtering of isotope signatures to reveal the source of chlorinated palm oil contaminants (2011)

This paper reports new insights at the molecular level into the route of a worldwide problem of the food industry: the occurrence of monochloro-propanediol (MCPD) esters. The application of mass defect-driven workflows is described to generate a hypothesis on the identity and occurrence of those thermally labile, chlorinated contaminant precursors that may act as chlorine donors during the formation of MCPD esters. For the first time, holistic mass-defect filtering of isotope signatures is used to pinpoint completely unknown and unexpected chlorine-containing substances naturally present in various extracts of palm fruit and partially and fully refined oils. Supervised multivariate analysis showed the effective classification of samples from various stages of industrial processing, suggesting that these steps strongly impact a complex and dynamic pool of chlorinated substances. In-vitro experiments confirmed that several of these naturally occurring chlorinated plant constituents decompose upon heat treatment, thus potentially being a source of chlorine for further reactions with palm oil lipids in a subsequent chlorination cascade. It is hypothesised that during oil refining the organochlorines naturally present in palm fruits act as a 'chlorine source' for the generation MCPD diesters. This discovery implies that industrial efforts targeting the mitigation of chlorinated substances must intervene at the earliest possible production stage or preferably even prior to oil processing. Current performance and limitations of mass-defect filtering are discussed and future developments are outlined.
Bit-parallel finite field multiplier and squarer using polynomial basis (2002)

Bit-parallel finite field multiplication using polynomial basis can be realized in two steps: polynomial multiplication and reduction modulo the irreducible polynomial. In this article, we present an upper complexity bound for the modular polynomial reduction. When the field is generated with an irreducible trinomial, closed form expressions for the coefficients of the product are derived in term of the coefficients of the multiplicands. The complexity of the multiplier architectures and their critical path length are evaluated, and they are comparable to the previous proposals for the same class of fields. An analytical form for bit-parallel squaring operation is also presented. The complexities for bit-parallel squarer are also derived when an irreducible trinomial is used. Consequently, it is argued that to solve multiplicative inverse using polynomial basis can be at least as good as using a normal basis.
Donor Calculi Induced Acute Renal Failure (2003)

Nephrolithiasis is an infrequent complication following renal transplantation and acquisition of a stone with the donor kidney is rare. Indeed only a few cases of donor stones causing renal failure have been reported. Since the grafted kidney is denervated, stone disease may not present with the classic renal colic but rather with acute renal failure secondary to the obstruction. Identification of the precise cause is critical in order to avoid inappropriate therapy. We present our experience of two renal transplant patients who developed obstructive uropathy by stones originating from the donor kidneys.
Quantitative Buffy Coat Analysis (1983)

We have developed a system for the quantitative analysis of the buffy coat in centrifuged whole blood samples. This analysis, performed in a modified microhematocrit tube, provides a hematocrit value, total WBC count, platelet count, and a separation of the leukocyte population into granulocytes and nongranulocytes. All results are available within 15 minutes and correlate well with existing methods. The system is expected to provide a rapid means of performing a complete blood cell count in a physician's office. (<i>JAMA</i>1983;249:617-620)
Human interleukin 2 analogues that preferentially bind the intermediate-affinity interleukin 2 receptor lead to reduced secondary cytokine secretion: implications for the use of these interleukin 2 analogues in cancer immunotherapy. (1993)

Cancer patients undergoing interleukin (IL)-2-based immunotherapy frequently experience dose-limiting side effects believed to be caused by the actions of such cytokines as IL-1 beta, tumor necrosis factor (TNF)-alpha and -beta, and interferon-gamma (IFN-gamma). Human peripheral blood mononuclear cells (PBMC) or monocyte-depleted peripheral blood lymphocytes were stimulated for up to 7 days by either of 2 IL-2 analogues (R38A or F42K) that bind to the intermediate-affinity IL-2 beta gamma receptor but have reduced abilities to bind the high-affinity IL-2 receptor. We previously reported that these IL-2 analogues retain the ability to generate lymphokine-activated killing by PBMC. In this study, we analyzed the cytokine content of supernatants from stimulated PBMC and peripheral blood lymphocyte cultures by enzyme-linked immunosorbent assay. The secretions of IL-1 beta, TNF-alpha, and -beta, and IFN-gamma induced by either R38A or F42K were markedly reduced compared with secretions produced in response to recombinant wild-type IL-2. In 4 experiments, secretion was reduced an average of 39% for IL-1 beta, 57% for TNF-alpha, 83% for TNF-beta, and 86% for IFN-gamma. Polymerase chain reaction analysis of recombinant wild-type IL-2 or analogue-stimulated PBMC did not reveal the presence of IL-2 mRNA; thus, differential production of endogenous IL-2 could not account for these findings. These data suggest the interaction of IL-2 and the high-affinity IL-2 receptor on human PBMC or peripheral blood lymphocyte is required for maximal secretion of IL-1 beta, TNF-alpha, TNF-beta, and IFN-gamma. Because such cytokines are believed to mediate the toxicity seen with IL-2-based immunotherapies, IL-2 analogues with reduced binding to the high affinity IL-2 receptor may prove to be an effective and less toxic means of cancer treatment.
Continuous-time digital signal processing (2003)

Digitally processing quantised (but not sampled) signals produces no aliasing and reduces the in-band quantisation error. Techniques for accomplishing such processing are proposed. Experimental evidence is included.
Optimal Use of E85 in a Turbocharged Direct Injection Engine (2009)

&lt;div class="htmlview paragraph"&gt;Ford Motor Company is introducing “EcoBoost” gasoline turbocharged direct injection (GTDI) engine technology in the 2010 Lincoln MKS. A logical enhancement of EcoBoost technology is the use of E85 for knock mitigation. The subject of this paper is the optimal use of E85 by using two fuel systems in the same EcoBoost engine: port fuel injection (PFI) of gasoline and direct injection (DI) of E85.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Gasoline PFI is used for starting and light-medium load operation, while E85 DI is used only as required during high load operation to avoid knock. Direct injection of E85 (a commercially available blend of ∼85% ethanol and ∼15% gasoline) is extremely effective in suppressing knock, due to ethanol's high inherent octane and its high heat of vaporization, which results in substantial cooling of the charge. As a result, the compression ratio (CR) can be increased and higher boost levels can be used. The increased full load BMEP allows downsizing of the engine at equivalent or enhanced vehicle performance.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;By enabling higher CR and engine downsizing, the use of E85 DI + gasoline PFI makes the engine more efficient in its use of gasoline, thereby leveraging the effect of the available ethanol in reducing the consumption of gasoline. This leveraging has a profound influence on ethanol's net energy balance and CO&lt;sub&gt;2&lt;/sub&gt; reduction potential. The vehicle owner will realize high fuel economy because gasoline, with its high heating value per volume, is primarily used for most driving modes in a downsized, high CR engine.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;In this paper, the concept of E85 DI + gasoline PFI is assessed using a Ford Motor Company 3.5L turbocharged direct injection “EcoBoost” engine. A PFI system was added to the engine and CR was increased to 12:1. The amount of E85 required to avoid knock was quantified as a function of BMEP at various engine speeds on an engine dynamometer. A full load torque curve subject to the peak pressure and turbine inlet temperature constraints of the engine was also acquired. A vehicle simulation program was then used to quantify the amount of E85 required for various drive cycles, and to determine vehicle fuel consumption.&lt;/div&gt;
Risk Factors for Doxorubicin-lnduced Congestive Heart Failure (1979)

Potential risk factors responsible for development of doxorubicin-induced congestive heart failure were examined through retrospective analysis of 4018 patient records. The overall incidence of drug-induced congestive heart failure was 2.2% (88 cases). The probability of incurring doxorubicin-induced congestive heart failure was related to the total dose of doxorubicin administered. There was a continuum of increasing risk as the cumulative amount of administered drug increased. A weekly dose schedule of doxorubicin was associated with a significantly lower incidence of congestive heart failure than was the usually employed every 3-week schedule. An increase in drug-related congestive heart failure was also seen with advancing patient age. Performance status, sex, race, and tumor type were not risk factors. These data will enable clinicians to better estimate the risk/benefit ratio in individual patients receiving prolonged administration of doxorubicin. They also provide a basis for the investigation of less cardiotoxic anthracycline analogues or for designing measures to prevent doxorubicin-induced cardiomyopathy.
Improving the Behavior of Bis(phenoxyamine) Group 4 Metal Catalysts for Controlled Alkene Polymerization (2009)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTImproving the Behavior of Bis(phenoxyamine) Group 4 Metal Catalysts for Controlled Alkene PolymerizationRoberta Cipullo†, Vincenzo Busico*†‡, Natascia Fraldi†‡, Roberta Pellecchia†‡, and Giovanni Talarico†View Author Information† Dipartimento di Chimica "Paolo Corradini", Università di Napoli Federico II, Via Cintia, 80126 Napoli, Italy‡ Dutch Polymer Institute (DPI), PO Box 902, 5600 AX Eindhoven, The Netherlands*Corresponding author. E-mail: [email protected]Cite this: Macromolecules 2009, 42, 12, 3869–3872Publication Date (Web):May 8, 2009Publication History Received31 March 2009Revised27 April 2009Published online8 May 2009Published inissue 23 June 2009https://pubs.acs.org/doi/10.1021/ma9006957https://doi.org/10.1021/ma9006957rapid-communicationACS PublicationsCopyright © 2009 American Chemical SocietyRequest reuse permissionsArticle Views1947Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Alkyls,Catalysts,Polymerization,Stereoselectivity,Thermoresponsive polymers Get e-Alerts
Split Anergy in a CD8 + T Cell: Receptor-dependent Cytolysis in the Absence of Interleukin-2 Production (1991)

Engagement of the antigen-specific receptor (TCR) of CD4+ T lymphocytes without a second (costimulatory) signal prevents the subsequent production of interleukin-2 (IL-2) by these cells. Because IL-2 is a key immunoregulatory lymphokine and is also produced by a subset of CD8+ T cells that are able to kill target cells, the effect of engaging the TCR of one such clone in the absence of costimulatory signals was examined. The capacity for TCR-dependent IL-2 production was lost, indicating comparable costimulator-dependent signaling requirements for IL-2 production in CD4+ and CD8+ T cells. However, TCR-mediated cytotoxicity was not impaired, implying that costimulation is required for only certain TCR-dependent effector functions.
Conditional enhancement of liver-specific gene transcription. (1988)

We sought to develop a cell line in which liver-specific transcription could be induced at will, to facilitate the study of factors that cause hepatocyte-specific transcription of the serum albumin gene in mice. We therefore created the H2.35 cell line from mouse hepatocytes infected with a temperature-sensitive strain of simian virus 40. During routine propagation at the permissive temperature, H2.35 cells exhibit extremely low levels of albumin transcription and mRNA. Albumin mRNA increases at least 100-fold when H2.35 cells are cultured at the restrictive temperature and in serum-free medium on a collagen substratum; the two latter conditions maintain the differentiated state of primary hepatocyte cultures. Although a major cause of the mRNA increase is posttranscriptional, the transcription rates of albumin and other liver-specific genes increase significantly. Transient-transfection experiments demonstrated that an induction of transcription is caused by activation of an albumin upstream sequence that was previously shown to enhance liver-specific transcription in transgenic mice. Thus, hepatocyte differentiation appears to be maintained in part by extracellular signals that stimulate the activity of a tissue-specific enhancer element.
The conformation of apolipoprotein A-I in discoidal and spherical recombinant high density lipoprotein particles. 13C NMR studies of lysine ionization behavior. (1992)

To elucidate the molecular details of how high density lipoprotein (HDL) microstructure affects the conformation of apolipoprotein (apo) A-I in various classes of HDL particles, apoA-I structure in homogeneous recombinant HDL (rHDL) complexes containing palmitoyl-oleoyl phosphatidylcholine (POPC) and cholesteryl oleate has been investigated by NMR spectroscopy of [13C]lysine-labeled apoA-I. All Lys residues in rHDL apoA-I were labeled with 13C by reductive methylation, and then their ionization behavior was characterized by 13C NMR spectroscopy. Four discoidal particles were prepared to contain from 64 to 256 molecules of POPC and 2 molecules of apoA-I; their major diameters ranged from 9.3 to 12.1 nm. (13CH3)2-Lys resonances from apoA-I in discoidal complexes exhibit six distinct chemical shifts at pH 10. The various Lys have pKa values ranging from 8.3 to 10.5, indicating that they exist in different microenvironments. More than 80% of the Lys residues in small (9.3 nm) discoidal particles titrate at a significantly lower pH than in the large (12.1 nm) discoidal particles. This indicates that apoA-I has a different conformation on the differently size discs. Two spherical particles were prepared with POPC:cholesteryl oleate:apoA-I molar stoichiometries of 56:16:2 and 232:84:4 and diameters of 7.4 and 12.6 nm, respectively. On spherical rHDL, apoA-I (13CH3)2-Lys resonances exhibit five distinct chemical shifts at pH 10. The titration behavior of apoA-I Lys residues is the same in small and large spherical particles, indicating that apoA-I conformation is similar on the two particles. The Lys microenvironments indicate that the conformation of apoA-I in discoidal complexes is dependent on particle size and that these conformations are substantially different from that of apoA-I on spherical complexes. Lys microenvironments in discoidal complexes differ from that of spherical complexes by 4 to 5 ysines which titrate with relatively low pKa values on discs. This reflects apparent differences in conformation in the NH2-terminal one-third of apoA-I on discs and spheres.