gebhart/patrepeval huggingface dataset:
Pertussis toxin-induced ADP ribosylation of inhibitor G proteins alters vagal control of heart rate in vivo (1993)

Prior studies have shown that in vivo systemic administration of pertussis toxin in conscious dogs catalyzes ADP ribosylation of Gi and G(o) proteins, which attenuates intracellular transduction of muscarinic receptor activation. We tested the hypothesis that this impairment may result in the alteration of the following indexes of cardiac vagal activity: baroreflex sensitivity and heart rate variability. Heart rates during submaximal exercise were also determined. These variables were measured in eight conscious dogs before and 72 hr after pertussis toxin administration. Pertussis toxin significantly reduced (P < 0.001) baroreflex sensitivity (from 18.7 +/- 2.6 to 6.8 +/- 1.4 ms/mmHg), the SD of the mean R-R intervals (from 176 +/- 17 to 61 +/- 7 ms), the mean R-R interval (from 742 +/- 32 to 527 +/- 29 ms), and the coefficient of variance [from 235 +/- 15 to 114 +/- 1 (x 1,000)]. The heart rate response to graded submaximal exercise after pertussis toxin was higher (P < 0.001) at each exercise level. In in vitro assay, cardiac tissue samples from pertussis toxin-treated dogs incorporated 10-fold less ADP ribose than what has been described previously. These data prove that the in vivo action of pertussis toxin on cardiac inhibitory G proteins has direct consequences on end-organ cardiac responses to vagal activity. This study quantifies the physiological consequences of pertussis toxin-induced impairment of inhibitory G proteins in conscious animals.
A STUDY OF THE EFFECT OF WEIGHT AND DIETARY FAT ON BREAST CANCER SURVIVAL TIME (1986)

Female breast cancer patients who had participated in a case-control study conducted during 1973-1975 were followed up, using record linkage to the Statistics Canada mortality data base, to the end of 1980. A number of variables recorded for the case-control study, in particular body weight and daily intake of dietary fat near the time of diagnosis, were examined to see if they had any bearing on breast cancer mortality. The data were analyzed using the methods of Mantel and Cox. Dietary fat was not found to be related to patient survival. However, cases with greater than average body weight experienced a shortened survival time.
Imaging Carbon Nanotubes in High Performance Polymer Composites via Magnetic Force Microscopy (2002)

Application of carbon nanotubes as reinforcement in structural composites is dependent on the efficient dispersion of the nanotubes in a high performance polymer matrix. The characterization of such dispersion is limited by the lack of available tools to visualize the quality of the matrix/carbon nanotube interaction. The work reported herein demonstrates the use of magnetic force microscopy (MFM) as a promising technique for characterizing the dispersion of nanotubes in a high performance polymer matrix.
Cationic lipids enhance cytokine and cell influx levels in the lung following administration of plasmid: cationic lipid complexes. (1998)

Administration of plasmid/lipid complexes to the lung airways may be associated, in addition to expression of transgene, with a range of other responses. We report here the induction of cytokines and cellular influx in the lung airway following intratracheal administration of an N-[1-(2-3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride/cholesterol/plasmid positively charged complex in mice. We show that 1) the appearance of the Th1-associated cytokines IFN-gamma and IL-12 in bronchoalveolar lavage fluid is caused by unmethylated CpG dinucleotide sequences present within the plasmid, and is enhanced by the lipid formulation; 2) cationic lipids by themselves do not induce IL-12 or IL-12p40; 3) TNF-alpha is rapidly induced by cationic lipids and plasmid/lipid complex, but not by plasmid alone; 4) an acute cellular influx is induced by cationic lipid alone and by a plasmid/lipid complex, but to a much lesser extent by plasmid alone; and 5) plasmid methylation does not influence the degree of inflammatory cell influx. The induction of the innate immune responses by plasmid/lipid complexes may be advantageous to gene therapy of lung diseases. In particular, induction of the Th1 cell-promoting cytokines by plasmid/lipid complexes could, in conjunction with an expressed transgene, be used to modulate immune responses in the lung airways in disease conditions that are deficient in Th1 cell responses or that have a dominant Th2 phenotype. Alternatively, the elimination of immunostimulatory sequences in plasmids may improve the tolerability and/or efficacy of nonviral gene therapy, especially for diseases requiring chronic administration.
Pergamum: replacing tape with energy efficient, reliable, disk-based archival storage (2008)

As the world moves to digital storage for archival purposes, there is an increasing demand for reliable, lowpower, cost-effective, easy-to-maintain storage that can still provide adequate performance for information retrieval and auditing purposes. Unfortunately, no current archival system adequately fulfills all of these requirements. Tape-based archival systems suffer from poor random access performance, which prevents the use of inter-media redundancy techniques and auditing, and requires the preservation of legacy hardware. Many diskbased systems are ill-suited for long-term storage because their high energy demands and management requirements make them cost-ineffective for archival purposes. Our solution, Pergamum, is a distributed network of intelligent, disk-based, storage appliances that stores data reliably and energy-efficiently. While existing MAID systems keep disks idle to save energy, Pergamum adds NVRAM at each node to store data signatures, metadata, and other small items, allowing deferred writes, metadata requests and inter-disk data verification to be performed while the disk is powered off. Pergamum uses both intra-disk and inter-disk redundancy to guard against data loss, relying on hash tree-like structures of algebraic signatures to efficiently verify the correctness of stored data. If failures occur, Pergamum uses staggered rebuild to reduce peak energy usage while rebuilding large redundancy stripes. We show that our approach is comparable in both startup and ongoing costs to other archival technologies and provides very high reliability. An evaluation of our implementation of Pergamum shows that it provides adequate performance. 1
Construction of Matryoshka‐Type Structures from Supercharged Protein Nanocages (2014)

Designing nanoscaled hierarchical structures with increasing levels of complexity is challenging. Here we show that electrostatic interactions between two complementarily supercharged protein nanocages can be effectively utilized to create nested Matryoshka-type structures. Cage-within-cage complexes containing spatially ordered iron oxide nanoparticles spontaneously self-assemble upon mixing positively supercharged ferritin compartments with AaLS-13, a larger shell-forming protein with a negatively supercharged lumen. Exploiting engineered Coulombic interactions and protein dynamics in this way opens up new avenues for creating hierarchically organized supramolecular assemblies for application as delivery vehicles, reaction chambers, and artificial organelles.
GDS-03 MULTI-OBJECTIVE OPTIMIZATION OF THE TOOTH SURFACE IN HELICAL GEARS USING THE RESPONSE SURFACE METHOD(DESIGN AND SYNTHESIS OF A PAIR OF GEARS OR OF GEAR TRAINS) (2009)

The design of tooth surface for a low noise under the various load condition is very important. Gear noise is highly related to the transmission error. The optimal tooth surface for the reduction of transmission error is very difficult to determine analytically due to nonlinearity of transmission error and the satisfaction of several load conditions. The satisfaction of design variables in several load conditions leads to Pareto optimum of multi-objective optimization. Therefore, this study proposes the method to determine the optimal lead curve and robust design of the tooth surface, using the response surface method and multi-objective optimization. To do so, the effect of transmission error on candidate design variables by a screening experiment has been investigated using an analysis of variance and the design variables are selected. Then the fitted regression model is built with the statistic validation of the model. The model with constraints is solved to obtain the optimum design of the lead curve and the robust design for the tooth surface.
Parallel transport subsystem implementation for high‐performance communication (1994)

Abstract Requirements of emerging applications together with rapid changes in networking technology towards gigabit speeds require new adequate transport systems. Integrated designs of transport services, protocol architecture and implementation platforms are required by forthcoming applications in high‐speed network environments. The transport subsystem PATROCLOS (parallel transport subsystem for cel/ based high‐speed networks) is designed with special emphasis on a high degree of inherent parallelism to allow efficient implementations on multiprocessor architectures combined with specialized hardware for very time critical functions. The paper presents the new parallel protocol architecture of PATROCLOS, an appropriate implementation architecture based on transputer networks, and performance evaluation results, which indicate high throughput values.
Effects of hepatic P-450 enzyme inhibitors and inducers on the duration of xylazine + ketamine anesthesia in broiler chickens and mice. (1993)

We previously showed that pretreatment with inhibitors of hepatic P-450 microsomal enzymes prolonged xylazine-ketamine anesthesia, which sometimes led to mortality in rats. In this study we determined if similar effects were produced in broiler chickens and mice. A combination of 5 mg xylazine/kg and 15 mg ketamine/kg im was given to broiler chickens 5-6 w of age, and a combination of 10 mg xylazine/kg and 200 mg ketamine/kg ip was given to mice. The loss of righting reflex was used to measure duration of anesthesia. Pretreatment with 100 mg chloramphenicol/kg im, 150 mg cimetidine/kg im, 25 mg SKF-525A/kg im or 40 mg ketoconazole/kg po significantly increased the duration of xylazine + ketamine anesthesia in the chickens. Pretreatment with 40 mg phenobarbital/kg im bid for 3 d, 25 mg 3-methylcholanthrene/kg im sid for 3 d, or 50 mg rifampin/kg im bid for 2 d failed to alter the duration of xylazine + ketamine anesthesia in the broilers. None of the inhibitors tested altered the duration of anesthesia in mice. Some chickens pretreated with inhibitors (cimetidine, ketoconazole, SKF-525A) or inducers (phenobarbital, 3-MC, rifampin) died. This study suggests that hepatic metabolism of xylazine may be similar in the rat and broiler chicken and that the pulmonary metabolism of xylazine and ketamine may be different in chickens and rats.
Evaluation of the effects of peptide antibiotics human β‐defensins‐1/‐2 and LL‐37 on histamine release and prostaglandin D2production from mast cells (2001)

Antimicrobial peptides, human beta-defensins (hBD-1/-2), and LL-37 (a peptide of human cathelicidin CAP18) are predominately expressed at epithelial tissues, where they participate in the innate host defense by killing invading microorganisms. In this study, to investigate the interactions between epithelial cell-derived antimicrobial peptides and mast cells, we evaluated the effects of hBD-1/-2 and LL-37 on mast cell functions using rat peritoneal mast cells. hBD-2 and LL-37 but not hBD-1 induced histamine release and intracellular Ca(2+) mobilization, and hBD-2 was more potent than LL-37. Interestingly, histamine release and intracellular Ca(2+) mobilization elicited by hBD-2 and LL-37 were markedly suppressed by BAPTA-AM (an intracellular Ca(2+) chelating agent), pertussis toxin and U-73122 (a phospholipase C inhibitor). In addition, among the peptides examined, only hBD-2 significantly induced PGD(2) production, which was abolished by indomethacin (cyclooxygenase-1/-2 inhibitor) but not NS-398 (cyclooxygenase-2 inhibitor), suggesting that hBD-2-induced PGD(2) production is mediated by cyclooxygenase-1. Likewise, the PGD(2) production was suppressed by pertussis toxin and U-73122. These observations suggest that hBD-2 and LL-37 stimulate mast cells to mobilize intracellular Ca(2+) and release histamine or generate PGD(2) in a G protein-phospholipase C-dependent manner. Thus, hBD-2 and LL-37 may have modulatory effects on inflammatory reactions.
Increased expression of eukaryotic translation initiation factors eIF-4E and eIF-2 alpha in response to growth induction by c-myc. (1993)

Although activation of c-myc is a critical step in the development of lymphomas and other tumors, its normal function(s) in cell growth remain obscure because few myc-regulated genes are known. myc expression normally increases in response to mitogens and peaks in G1 when additional protein synthesis is required for cell-cycle progression. Protein synthesis is controlled by the availability of translation initiation factors, including the mRNA cap binding protein (eIF-4E) and the alpha subunit of the eIF-2 complex that binds the initiator Met-tRNA. Consequently we examined eIF-4E and eIF-2 alpha for evidence of regulation by c-myc. Expression of eIF-4E and eIF-2 alpha correlated with c-myc expression in fibroblasts after growth stimulation. In addition, expression of eIF-4E and eIF-2 alpha was increased in myc-transformed rat embryo fibroblasts but was not increased in ras-transformed cells. Transcription rates of eIF-4E and eIF-2 alpha mRNAs were regulated by c-myc in cells expressing an estrogen receptor-Myc fusion protein. Finally, electrophoretic mobility-shift assays identified a sequence element in the eIF-2 alpha promoter, TCCGCAT-GCGCG, which was specifically retarded by extracts of myc-expressing cells. c-myc is thought to deregulate the growth of cancer cells by activating transcription, suggesting that specific genes regulated by c-myc should also function as oncogenes. In previous studies these translation initiation factors could induce neoplastic growth because overexpression of eIF-4E-transformed cells and inhibition of a suppressor of eIF-2 alpha (eIF-2 alpha kinase) also caused malignant transformation. Our studies suggest that one important biological function of c-myc may be to increase cell growth by increasing expression of eIF-4E and eIF-2 alpha.
Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the purification of a biosynthetic intermediate (1997)

Methylobacterium extorquens AM1 produces pyrroloquinoline quinone (PQQ), the prosthetic group of methanol dehydrogenase. Two gene clusters have been shown to be required for PQQ biosynthesis in this micro-organism and complementation analysis has identified seven pqq genes, pqqDGCBA and pqqEF. The DNA sequence of pqqDGC’ was reported previously. This paper reports the sequence of the genomic region corresponding to pqqC'BA. For consistency, the nomenclature of pqq genes in Klebsiella pneumoniae will be followed. The new nomenclature for pqq genes of M. extorquens AM1 is pqqABCDE and pqqFG. In the genomic region sequenced in this study, two open reading frames were found. One of these encodes PqqE, which showed high identity to analogous pqq genes in other bacteria. PqqE also showed identity to MoaA and NifB in the N-terminal region, where a conserved CxxxCxYC sequence was identified. The sequence of the second open reading frame covered both the pqqC and pqqD regions, suggesting that both functions were encoded by this gene. It is proposed to designate this gene pqqC/D. The deduced amino acid sequence of the pqqC/D product showed identity to PqqC of K. pneumoniae and Pqql of Acinetobacter calcoaceticus in the N-terminal region, and to PqqD of K. pneumoniae and Pqqll of A. calcoaceticus in the C-terminal region. A fragment of M. extorquens AM1 DNA containing only pqqC/D produced a protein of 42 kDa in Escherichia coli, which corresponds to the size of the deduced amino acid sequence of PqqC/D, confirming the absence of a separate pqqD. This genomic region complemented the growth of pqqC mutants of M. extorquens AM1 and Methylobacterium organophilum DSM 760 on methanol. As previously reported for pqq genes of K. pneumoniae, a pqqC mutant of M. extorquens AM1 produced an intermediate of PQQ biosynthesis, which was converted to PQQ by incubation with a crude extract from E. coli cells expressing PqqC/D. The intermediate was found in both crude extract and culture supernatant, and it was purified from the crude extract. The PqqC/D enzyme reaction appeared to require molecular oxygen and reduced nicotinamide adenine dinucleotides.
Pressure Dependence of the Carbon Dioxide/Cyclohexene Oxide Coupling Reaction Catalyzed by Chromium Salen Complexes. Optimization of the Comonomer-Alternating Enchainment Pathway (2004)

The rate of the copolymerization reaction of cyclohexene oxide and carbon dioxide in the presence of (salen)CrIIIN3 and various cocatalysts has been determined as a function of CO2 pressure. Carbon dioxide insertion into the (salen)Cr-alkoxide intermediates, afforded following epoxide ring-opening, was shown to be rate-limiting at pressures below 35 bar. Higher pressures of carbon dioxide resulted in catalyst/substrate dilution with a concomitant decrease in the rate of copolymer formation. On the other hand, cyclic carbonate formation was inhibited as the CO2 pressure was increased. The most active (salen)CrN3 catalyst (H2salen = N,N‘-bis(3-tert-butyl-5-methoxysalicylidene)-(1R,2R)-cyclohexenediimine), along with a [PPN][N3] cocatalyst, exhibited a TOF of 1153 mol epoxide consumed/mol chromium·h at 80 °C and a CO2 pressure of 34.5 bar.
CCL18 as an indicator of pulmonary fibrotic activity in idiopathic interstitial pneumonias and systemic sclerosis (2007)

These findings suggest that CCL18 production by BAL cells and serum CCL18 concentrations reflect pulmonary fibrotic activity in patients with IIPs and those with SSc. Monitoring changes in CCL18 production might be an extraordinarily useful tool in clinical practice and in studies aimed at evaluating new approaches for treatment of fibrotic lung diseases.
Lymphotoxin and LIGHT signaling pathways and target genes (2004)

Lymphotoxins (LT alpha and LT beta), LIGHT [homologous to LT, inducible expression, competes with herpes simplex virus (HSV) glycoprotein D for HSV entry mediator (HVEM), a receptor expressed on T lymphocytes], tumor necrosis factor (TNF), and their specific receptors LT beta R, HVEM, and TNF receptor 1 (TNFR1) and TNFR2, form the immediate family of the larger TNF superfamily. These cytokines establish a critical communication system required for the development of secondary lymphoid tissues; however, knowledge of the target genes activated by these signaling pathways is limited. Target genes regulated by the LT alpha beta-LT beta R pathway include the tissue-organizing chemokines, CXCL13, CCL19, and CCL21, which establish cytokine circuits that regulate LT expression on lymphocytes, leading to organized lymphoid tissue. Infectious disease models have revealed that LT alpha beta pathways are also important for innate and adaptive immune responses involved in host defense. Here, regulation of interferon-beta by LT beta R and TNFR signaling may play a crucial role in certain viral infections. Regulation of autoimmune regulator in the thymus via LT beta R implicates LT/LIGHT involvement in central tolerance. Dysregulated expression of LIGHT overrides peripheral tolerance leading to T-cell-driven autoimmune disease. Blockade of TNF/LT/LIGHT pathways as an intervention in controlling autoimmune diseases is attractive, but such therapy may have risks. Thus, identifying and understanding the target genes may offer an opportunity to fine-tune inhibitory interventions.
Association of a tumor necrosis factor promoter polymorphism with susceptibility to alcoholic steatohepatitis (1997)

Twin concordance studies suggest that genetic factors play a role in determining why only a minority of heavy drinkers develop hepatitis and cirrhosis. Tumor necrosis factor alpha (TNF-alpha) has emerged as the "final common pathway" in the pathogenesis of alcohol-related hepatic necro-inflammation. We have examined the frequency of the two recently described polymorphisms of the TNF-alpha promoter in 150 patients with biopsy-proven alcoholic liver disease and 145 healthy volunteers. There was a significant excess of the rare allele (TNFA-A; G(-238) --> A) at position -238 in patients with steatohepatitis compared with controls or patients without this lesion. This is consistent with previous suggestions that the TNFA-A allele, which falls within a putative Y regulation box of the TNF-alpha promoter, is associated with increased TNF-alpha expression. No differences were observed for the polymorphism at position -308.
Why Do Skin Grafts Fail? (1979)

Fibrin is shown to be the agent responsible for the adherence of biological dressings and of autografts to wounds. Its presence is associated with graft success, and its absence with graft failure. The results suggest that the deposition of fibrin provides the basis for the anti-bacterial actions of biological dressings and for the sterilization of the wound under adherent autografts. The total number of bacteria per gram of tissue in the wound, though important, is not critical to the result of skin grafting. The mechanism by which different organisms cause grafts to fail is by the production of plasmin and proteolytic enzymes which dissolve the important fibrin scaffold--thus ensuring their own survival. Thus, it is the levels of these (and the numbers of organisms efficient in producing them) which cause success or failure of applied skin grafts.
Synthesis and in vitro‐Anticancer and Antimicrobial Evaluation of Some Novel Quinoxalines Derived from 3‐Phenylquinoxaline‐2(1H)‐thione (2006)

Two novel series derived from 3-phenylquinoxaline-2(1H)-thione 2 and 2-(hydrazinocarbonylmethylthio)-3-phenylquinoxaline 6 have been synthesized. Eight out of twenty six new compounds were selected at the National Cancer Institute for evaluation of their in vitro-anticancer activity. Among them, compounds 3b, 3c, 4b, and 4c displayed moderate to strong growth inhibition activity against most of the tested sub-panel tumor cell lines with GI(50) 10(-5) to 10(-6 )molar concentrations. Compound 4b exhibited a significant value of percent tumor growth inhibition against breast cancer at concentration < 10(-8) M. Compound 4c showed moderate selectivity towards leukemia cell lines with GI(50) of 1.8 to 3.8 microM (selectivity ratio = 5.7). Preliminary antimicrobial testing revealed that compounds 7a, 7b, 8a, 11a, and 11b were as active as ampicillin against B. subtilis (MIC = 12.5 microg/mL). Compounds 7b and 8a were also nearly as active as ampicillin against E. coli (MIC = 12.5 microg/mL). In addition, compounds 4a, 7b, 10b, and 11a were as active as ampicillin against P. aerugenosa (MIC = 50 microg/mL). However, compounds 7b, 8a, and 10b showed mild activity against C. albicans (MIC = 50 microg/mL). The values of minimum bactericidal concentrations indicated that compounds 4a and 7b were bactericidal against B. subtilis and P. aerugenosa, respectively, while compound 10b was bactericidal against both organisms. However, compound 11a was bactericidal against E. coli, P. aerugenosa, and S. aureus.