gebhart/patrepeval huggingface dataset:
New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling (2005)

To gain more insight into initiation and regulation of T cell receptor (TCR) gene rearrangement during human T cell development, we analyzed TCR gene rearrangements by quantitative PCR analysis in nine consecutive T cell developmental stages, including CD34+ lin− cord blood cells as a reference. The same stages were used for gene expression profiling using DNA microarrays. We show that TCR loci rearrange in a highly ordered way (TCRD-TCRG-TCRB-TCRA) and that the initiating Dδ2-Dδ3 rearrangement occurs at the most immature CD34+CD38−CD1a− stage. TCRB rearrangement starts at the CD34+CD38+CD1a− stage and complete in-frame TCRB rearrangements were first detected in the immature single positive stage. TCRB rearrangement data together with the PTCRA (pTα) expression pattern show that human TCRβ-selection occurs at the CD34+CD38+CD1a+ stage. By combining the TCR rearrangement data with gene expression data, we identified candidate factors for the initiation/regulation of TCR recombination. Our data demonstrate that a number of key events occur earlier than assumed previously; therefore, human T cell development is much more similar to murine T cell development than reported before.
The Zonula, Lens, and Circumlental Space in the Normal Iridectomized Rhesus Monkey Eye (2006)

Characterization of age-related changes in the accommodative apparatus may help to model the system for hypothesis testing. The CLS may be an indicator of presbyopia-related processes in surrounding tissues. However, these results do not prove that the width of the CLS, in and of itself, has a causal relationship with accommodative amplitude, or that changes in the CLS play a pathophysiological role in presbyopia.
Using Feedforward Neural Networks to Monitor Alertness from Changes in EEG Correlation and Coherence (1995)

We report here that changes in the normalized electroencephalographic (EEG) cross-spectrum can be used in conjunction with feedforward neural networks to monitor changes in alertness of operators continuously and in near-real time. Previously, we have shown that EEG spectral amplitudes covary with changes in alertness as indexed by changes in behavioral error rate on an auditory detection task [6,4]. Here, we report for the first time that increases in the fre~uencv of detection errors in this task are also accompanied by patterns of increased and decreased spectral coherence in several frequency bands and EEG channel pairs. Relationships between EEG coherence and performance vary between subjects, but within subjects, their topographic and spectral profiles appear stable from session to session. Changes in alertness also covary with changes in correlations among EEG waveforms recorded at different scalp sites, and neural networks can also estimate alertness from correlation changes in spontaneous and unobtrusivelyrecorded EEG signals. 1
Human monoclonal antibodies produced by primary in vitro immunization of peripheral blood lymphocytes. (1988)

A general procedure is described for the production of human monoclonal antibodies from peripheral blood lymphocytes immunized in vitro against T-cell-dependent antigens. These lymphocytes immunized in culture were used to produce human-human or human-mouse hybridomas secreting monoclonal antibodies specific for digoxin, hemocyanin, a recombinant fragment of the gp120 envelope glycoprotein of human immunodeficiency virus (PB1), or a melanoma-associated antigen (p97). Depletion of a lysosome-rich cell population, containing large granular lymphocytes, monocytes, cytotoxic T cells, and a subset of CD8-positive T cells, was shown to be crucial before the cells could be immunized in vitro. This depletion was accomplished by treating the peripheral blood lymphocytes with the lysosomotropic agent L-leucine methyl ester. In addition, the in vitro immunization had to be supported by interleukin 2, gamma-interferon, and B-cell growth and differentiation factors, derived from irradiated, pokeweed-mitogen-stimulated human T cells. The production of human monoclonal antibodies from primary, antigen-specifically activated peripheral lymphocytes might obviate the need to immunize volunteers or patients.
CAPTOR: a model for delivering Web-based intelligent tutoring system technology (2002)

The goal of the Crisis Action Planning Tutored On-line Resource (CAPTOR) project is to design, develop, and implement a state-of-the-art, Internet-based course of instruction that utilizes Intelligent Tutoring System (ITS) technology. ITS technology is ideally suited to teach complex, cognitive tasks, such as those required for troubleshooting, problem-solving, and for resolving critical situations. As currently taught, Crisis Action Planning is part of an Armed Forces Staff College 12-week classroom course. It is projected that fully implementing CAPTOR as a distance learning ITS will dramatically reduce instructional training time.
Maleic Anhydride Modified Polypropylene with Controllable Molecular Structure:  New Synthetic Route via Borane-Terminated Polypropylene (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTMaleic Anhydride Modified Polypropylene with Controllable Molecular Structure: New Synthetic Route via Borane-Terminated PolypropyleneBing Lu and T. C. ChungView Author Information Department of Materials Science and Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802 Cite this: Macromolecules 1998, 31, 17, 5943–5946Publication Date (Web):August 6, 1998Publication History Received24 March 1998Revised27 May 1998Published online6 August 1998Published inissue 1 August 1998https://pubs.acs.org/doi/10.1021/ma9804693https://doi.org/10.1021/ma9804693rapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views787Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Amides,Copolymers,Polymers,Styrenes,Thermoresponsive polymers Get e-Alerts
Generalized autofocus (2011)

All-in-focus imaging is a computational photography technique that produces images free of defocus blur by capturing a stack of images focused at different distances and merging them into a single sharp result. Current approaches assume that images have been captured offline, and that a reasonably powerful computer is available to process them. In contrast, we focus on the problem of how to capture such input stacks in an efficient and scene-adaptive fashion. Inspired by passive autofocus techniques, which select a single best plane of focus in the scene, we propose a method to automatically select a minimal set of images, focused at different depths, such that all objects in a given scene are in focus in at least one image. We aim to minimize both the amount of time spent metering the scene and capturing the images, and the total amount of high-resolution data that is captured. The algorithm first analyzes a set of low-resolution sharpness measurements of the scene while continuously varying the focus distance of the lens. From these measurements, we estimate the final lens positions required to capture all objects in the scene in acceptable focus. We demonstrate the use of our technique in a mobile computational photography scenario, where it is essential to minimize image capture time (as the camera is typically handheld) and processing time (as the computation and energy resources are limited).
Bee declines driven by combined stress from parasites, pesticides, and lack of flowers (2015)

Bees are subject to numerous pressures in the modern world. The abundance and diversity of flowers has declined; bees are chronically exposed to cocktails of agrochemicals, and they are simultaneously exposed to novel parasites accidentally spread by humans. Climate change is likely to exacerbate these problems in the future. Stressors do not act in isolation; for example, pesticide exposure can impair both detoxification mechanisms and immune responses, rendering bees more susceptible to parasites. It seems certain that chronic exposure to multiple interacting stressors is driving honey bee colony losses and declines of wild pollinators, but such interactions are not addressed by current regulatory procedures, and studying these interactions experimentally poses a major challenge. In the meantime, taking steps to reduce stress on bees would seem prudent; incorporating flower-rich habitat into farmland, reducing pesticide use through adopting more sustainable farming methods, and enforcing effective quarantine measures on bee movements are all practical measures that should be adopted. Effective monitoring of wild pollinator populations is urgently needed to inform management strategies into the future.
Long-term safety and durable antiretroviral activity of lopinavir/ritonavir in treatment-naive patients (2004)

LPV/r-based therapy provides durable antiretroviral response and is generally well tolerated through 204 weeks of therapy.
Phase I and Pharmacokinetic Study of Bortezomib in Combination with Idarubicin and Cytarabine in Patients with Acute Myelogenous Leukemia (2008)

The combination of bortezomib, idarubicin, and cytarabine showed a good safety profile. The recommended dose of bortezomib for phase II studies with idarubicin and cytarabine is 1.5 mg/m(2).
Effects of Short-Term Dietary Restriction on Survival of Mammary Ascites Tumor-Bearing Rats (1988)

We studied the effects of short-term dietary restriction on the survival of 3-4-month-old tumor-free and tumor-bearing Fisher rats. The diet-restricted food regimen consisted of alternate day ad libitum feeding followed by alternate day fasting. Diet-unrestricted control rats were fed ad libitum daily. Six tumor-free rats on the diet-restricted regimen compensated for the dietary restriction by an increase in food consumption during the alternate feeding days, and lost an average of only 2-3% of their weight in 13 days. Six tumor-free rats on a daily ad libitum feeding regimen gained an average of 6.8% in 15 days. The above dietary-restricted regimen was initiated 1 week before 24 rats were inoculated intraperitoneally with 15 million Mat 13762 ascites tumor cells. Sixteen of 24 (66.7%) diet-restricted tumor-bearing hosts and 5/24 (20.8%) diet-unrestricted tumor-bearing hosts survived at 9 days after tumor inoculation (p less than 0.005). Twelve of 24 (50%) diet-restricted tumor-bearing hosts, and 3 of 24 (12.5%) diet-unrestricted tumor-bearing hosts, survived at 10 days after tumor inoculation (p less than 0.025). Thus, the survival of tumor-bearing rats was enhanced by short-term relatively mild dietary restrictions. We suggest that relatively mild dietary restrictions should be included in clinical trials designed to inhibit cancer growth and enhance the survival of human cancer patients.
Procalcitonin in Young Febrile Infants for the Detection of Serious Bacterial Infections (2008)

Procalcitonin has favorable test characteristics for detecting serious bacterial infections in young febrile infants. Procalcitonin measurements performed especially well in detecting the most serious occult infections.
A Mathematical Approach to Disaster Recovery Planning (2005)

It is crucial for highly security-critical information systems to accurately control the disaster recovery activities. Using optimization theory, we present a disaster recovery planning mathematical model. The model uses mathematical method to express diverse entities in information and disaster recovery system such as applications, facilities, resources, sub disaster recovery plans, budget, etc. It classifies various resources according to their utilization and payment, and identifies the significance of applications and the conflicts among sub disaster recovery plans. Through expressing relationships among applications, facilities, resources and sub plans, this model selects the best set of sub plans. This model has less subjective parameter and can evaluate disaster recovery planning objectively and quantitatively.
A microdroplet-based shift register (2010)

A microfluidic device is presented for the serial formation, storage and retrieval of water microdroplets in oil. The principle of operation is similar to that of an electronic shift register. Droplets, considered as units of information, can be arrayed and serially shifted within the device, allowing the controllable positioning of the emulsions and the creation of interfaces between drops. Using this passive system, by exploiting the balance between hydrodynamic pressure and surface tension across a drop due to the device design, droplet networks can be readily arrayed in a series of elements and cascaded within the microchannels in an automatable and high throughput fashion. The results showed the suitability of the system to be used for the formation of artificial lipid bilayers and for the study of biological dynamic processes based on the diffusion of molecules through interfaces.
Role of Aryl Hydrocarbon Receptor in Mesencephalic Circulation Failure and Apoptosis in Zebrafish Embryos Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (2003)

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and potent developmental toxicant in various animals, with developing fish being the most sensitive organisms. Although the expression of aryl hydrocarbon receptor (AHR) as well as the partner molecule, AHR nuclear translocator (ARNT) in the brain has been reported, the effect of TCDD on the brain remains to be clarified in detail. Previously, we reported local circulation failure and apoptosis in dorsal midbrain caused by TCDD in developing zebrafish. In the present experiments, we investigated the effects of morpholino antisense oligos against aryl hydrocarbon receptor 2 (zfAHR2) (AHR2-MO) on toxicological endpoints caused by TCDD in developing zebrafish. AHR2-MO but not its negative homologue (4mis-AHR2-MO) improved TCDD-evoked circulation failure in mesencephalic vein and reduced the occurrence of apoptosis in dorsal midbrain, with concomitant inhibition of CYP1A induction in vascular endothelium. Injection of bovine serum albumin (BSA) into the general circulation, followed by immunohistochemistry with anti-BSA, showed that TCDD raised vascular permeability to albumin in dorsal midbrain, which was blocked by AHR2-MO and N-acetlycystein. In the absence of TCDD, development of embryos injected with AHR2-MO appeared normal at least until 60 h after fertilization. It is concluded that AHR2 activation in the vascular endothelium of the zebrafish embryo midbrain is involved in the mesencephalic circulation failure and apoptosis elicited by TCDD. This is the further evidence that vascular endothelium is the target of TCDD in relation to local circulation failure and apoptosis in dorsal midbrain.
THE “GENERALIZED ROTOR” CRYPTOGRAPHIC OPERATOR AND SOME OF ITS APPLICATIONS (1985)

Computer implementations of linked substitution lists modulated by pseudo-random sequences (generalized rotors) are proposed as cryptographic substitution and transposition operators. Generalized Rotors are shown to have many-to-one mappings of the internal state to the output, to be well suited for substitution and transposition applications in cryptography, and to be excellent, hard to invert mixers of pseudo-random sequences.
Peptide Vaccination (1999)

T Lymphocytes are capable of eradication of both virus- and non-virus induced tumor cells [1, 2]. The highly diverse repertoire of T cells and the increased knowledge of the recognized ligands make it possible to design protocols to specifically activate tumor-specific T cells in vivo. By T cell receptor interaction, T lymphocytes can recognize MHC molecules, presenting antigen-derived peptide fragments. The identification of tumor-associated antigens and antigenic peptides has firmly established the existence of tumor specific T cell targets [3].
Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. (1982)

Mice carrying the Ipr mutation develop massive lymphoadenopathy and severe autoimmune disease. The characteristics of the cell population that proliferates in lymphoid tissues were evaluated by the use of a) monoclonal antibodies and FMF, and b) molecular genetic studies of Ig heavy chain genes. The lymph node cells of different strains of mice homozygous for the Ipr mutation were shown to be almost uniformly Thy-1+, Ly-1+, Ly-2-, H-11+, Ly-5+, sIg-, ThB-, 2C2+, I-A-, 6B2+, and therefore to have surface characteristics of both T and B cells. Molecular genetic studies of the arrangements of Ig heavy chain genes showed that they were not rearranged as in pre-B and B cells. These results suggest that an abnormal proliferating population of T cells in Ipr/Ipr mice aberrantly express B cell surface markers.
In Cardiomyocytes, Binding of Unzipping Peptide Activates Ryanodine Receptor 2 and Reciprocally Inhibits Calmodulin Binding (2012)

DPc10-RyR2 binding is sterically limited by the resting zipped RyR2 state. CaM binding to RyR2 stabilizes this zipped state, whereas RyR2 activation or prebound DPc10 enhances DPc10 access. DPc10-binding and CaM-binding sites are distinct but are allosterically interacting RyR2 sites. Neither DPc10 nor FKBP12.6 influences RyR2 binding of the other.
β-Peptides:  From Structure to Function (2001)

ADVERTISEMENT RETURN TO ISSUEPREVArticleβ-Peptides: From Structure to FunctionRichard P. Cheng, Samuel H. Gellman, and William F. DeGradoView Author Information Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6059, and Department of Chemistry, University of Wisconsin at Madison, Madison, Wisconsin 53706 Cite this: Chem. Rev. 2001, 101, 10, 3219–3232Publication Date (Web):October 10, 2001Publication History Received27 June 2001Published online10 October 2001Published inissue 1 October 2001https://pubs.acs.org/doi/10.1021/cr000045ihttps://doi.org/10.1021/cr000045iresearch-articleACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views12760Altmetric-Citations1704LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Amides,Chemical structure,Conformation,Monomers,Peptides and proteins Get e-Alerts
Alison Balter's Mastering Microsoft Office Access 2003 (2004)

What Alison's book offers over other books in that she is able to take a highly technical topic and present it in a manner that is easy to comprehend. It is a book that the reader will often want to read from cover to cover, but it can also act as an excellent reference. Readers of this book will learn: Access 11 application development and real-world solutions to specific development and programming problems. Professional programming techniques backed by concise, no-nonsense explanations of the underlying theories. Debugging and troubleshooting methods to solve problems quickly and get stalled development projects back on track.
An implementation of elliptic curve cryptosystems over F/sub 2/155 (1993)

The authors describe a VLSI Galois field processor and how it can be applied to the implementation of elliptic curve groups. They demonstrate the feasibility of constructing very fast, and very secure, public key systems with a relatively simple device, and the possibility of putting such a system on a smart card. The registers necessary to implement the elliptic curve system will require less than 1 mm/sup 2/ (or less than 4%) of the area available on the card.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Retinoic Acid Reverses Ethanol‐Induced Cardiovascular Abnormalities in Quail Embryos (1997)

The effect of ethanol on early avian cardiovascular development was investigated in stage 8 quail embryos grown in culture for 24 hr. When the culture medium contained 1% ethanol, 50% of the embryos developed abnormalities of the cardiovascular system, some of which resembled vitamin A deficiency. Only 15% of the embryos grown in control media developed abnormalities attributed to the manipulation of the embryo. When all-trans-retinoic acid, the active form of vitamin A, was added at 10(-8) M to the ethanol-containing medium, the cardiovascular development was similar to that of untreated controls. Inclusion of 4-methylpyrazole and citral, enzyme inhibitors for the conversion of retinol to retinoic acid, produced cardiovascular abnormalities in embryos similar to those observed in vitamin A deficiency. These abnormalities were partially prevented by the presence of 10(-8) M all-trans-retinoic acid in the medium. Immunohistochemical studies using antibodies specific for the heart muscle myosin heavy chain (MF-20) and quail endothelial cells (QH-1) revealed that looping of the heart of ethanol-treated embryos was prevented, and the embryonal circulation had no or minimal vascular connections to the extraembryonic circulation. Our studies provide indirect evidence that ethanol is producing vitamin A deficiency during embryonic cardiovascular development and that these effects are specifically prevented by the presence of retinoic acid. These findings may explain some of the symptoms of fetal alcohol syndrome.