gebhart/patrepeval huggingface dataset:
Tubular Membrane Cathodes for Scalable Power Generation in Microbial Fuel Cells (2007)

One of the greatest challenges for using microbial fuel cells (MFCs) for wastewater treatment is creating a scalable architecture that provides large surface areas for oxygen reduction at the cathode and bacteria growth on the anode. We demonstrate here a scalable cathode concept by showing that a tubular ultrafiltration membrane with a conductive graphite coating and a nonprecious metal catalyst (CoTMPP) can be used to produce power in an MFC. Using a carbon paper anode (surface area Aan = 7 cm2, surface area per reactor volume Aan,s = 25 m2/m3), an MFC with two 3-cm tube cathodes (Acat = 27 cm2, Acat,s = 84 m2/m3) generated up to 8.8 W/m3 (403 mW/m2) using glucose [0.8 g/L in a 50 mM phosphate buffer solution (PBS)], which was only slightly less than that produced using a carbon paper cathode with a Pt catalyst (9.9 W/m3, 394 mW/m2; Acat= 7 cm2, Acat,s= 25 m2/m3). Coulombic efficiencies (CEs) with carbon paper anodes were 25-40% with tube cathodes (CoTMPP), compared to 7-19% with a carbon paper cathode. When a high-surface-area graphite brush anode was used (Aan = 2235 cm2, Aan,s = 7700 m2/m3) with two tube cathodes placed inside the reactor (Acat = 27 cm2, Acas, = 93 m2/m3), the MFC produced 17.7 W/m3 with a CE = 70-74% (200 mM PBS). Further increases in the surface area of the tube cathodes to 54 cm2 (120 m2/m3) increased the total power output (from 0.51 to 0.83 mW), but the increase in volume resulted in a constant volumetric power density (approximately 18 W/m3). These results demonstrate that an MFC design using tubular cathodes coated with nonprecious metal catalysts, and brush anodes, is a promising architecture that is intrinsically scalable for creating larger systems. Further increases in power output will be possible through the development of cathodes with lower internal resistances.
국화의 효율적인 기내 발근 (1998)

Efficient in vitro rooting system of shoot cuttings in chrysanthemum was developed. After 1 or 2 weeks in culture, the rooting of shoots cultured on 1/4MS medium were obtained and influenced largely by IBA and sucrose concentration. In IBA treatments root
Expanding the Role of Neuromodulation for Overactive Bladder: New Indications and Alternatives to Delivery (2010)

From the time that it was granted US Food and Drug Administration approval, neuromodulation has secured a firm position in the treatment algorithm for overactive bladder. With neuromodulation, physicians were able to bridge the gap between the two ends of the treatment spectrum (medical therapy and open surgery). Sacral nerve stimulation has been the most widely used form of neuromodulation. Recent modifications to its design, namely the development of the tined lead and the launching of the refined InterStim II (Medtronic, Minneapolis, MN), have made sacral nerve simulation even less invasive and more effective. While InterStim is maintaining a level of success with these advancements, peripheral means of neuromodulation are being explored. The current literature takes a closer look at posterior tibial and pudendal nerve stimulation as alternatives to sacral nerve stimulation. The field of neuromodulation is also expanding in terms of the target patient population, as it is being used to treat children, patients with neurological disease, and others. As the role of neuromodulation increases, we must continue to assess its efficacy, safety, and cost-effectiveness in comparison to other therapeutic options.
Synthetic Affibody Molecules: A Novel Class of Affinity Ligands for Molecular Imaging of HER2-Expressing Malignant Tumors (2007)

The Affibody molecule Z(HER2:342-pep2), site-specifically and homogeneously conjugated with a 1,4,7,10-tetra-azacylododecane-N,N',N'',N'''-tetraacetic acid (DOTA) chelator, was produced in a single chemical process by peptide synthesis. DOTA-Z(HER2:342-pep2) folds spontaneously and binds HER2 with 65 pmol/L affinity. Efficient radiolabeling with >95% incorporation of (111)In was achieved within 30 min at low (room temperature) and high temperatures (up to 90 degrees C). Tumor uptake of (111)In-DOTA-Z(HER2:342-pep2) was specific for HER2-positive xenografts. A high tumor uptake of 23% injected activity per gram tissue, a tumor-to-blood ratio of >7.5, and high-contrast gamma camera images were obtained already 1 h after injection. Pretreatment with Herceptin did not interfere with tumor targeting, whereas degradation of HER2 using the heat shock protein 90 inhibitor 17-allylamino-geldanamycin before administration of (111)In-DOTA-Z(HER2:342-pep2) obliterated the tumor image. The present results show that radiolabeled synthetic DOTA-Z(HER2:342-pep2) has the potential to become a clinically useful radiopharmaceutical for in vivo molecular imaging of HER2-expressing carcinomas.
The Liaison network multimedia workstation (2002)

A description is given of a workstation architecture, Liaison, that solves the high bandwidth problem for real-time video and allows the exploration of these communications issues. By distributing the workstation's intelligence over a high-performance network, both the computation and communication load are shed to other processors on the network. The Liaison display performs only the low-level functions of acquiring and displaying images, whereas the remainder of the workstation's functionality is performed by a pool of distributed processors. Initial experiences with this architecture using a prototype monochrome workstation have provided insight into the issues surrounding the control and processing of communications-intensive applications.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Reduction in amyloid A amyloid formation in apolipoprotein-E-deficient mice. (1998)

Apolipoproteins have been implicated in the formation of amyloid fibrils. Recent studies have demonstrated that apolipoprotein E (apoE), alone or in combination with apolipoprotein J (apoJ), and other lipoproteins appear to enhance deposition of amyloid fibrils both in systemic and cerebral amyloids, especially Alzheimer's disease (AD). ApoE enhanced the ability of the amyloid beta-protein (1-40) fragment (A beta) to form fibrils in vitro, with apoE4 promoting the greatest fibril formation. ApoE was found associated with both human and mouse amyloid A (AA) deposits. To define the role of apoE in vivo, we utilized mice lacking the apoE gene by gene targeting. We used the AA model in mice to characterize the function of the apoE protein in amyloid fibrillogenesis. ApoE-deficient mice exhibited a decrease in deposition of AA when compared with heterozygous mutant or wild-type animals. In addition, apoE-deficient mice that were injected with an adenovirus that expressed the human apoE3 gene had restored AA deposition and the apoE was associated with the AA fibrils. These results are agreement with the in vitro studies using the beta-peptide and suggest that apoE is not essential for amyloid fibrillogenesis but can promote the development of amyloid deposition.
Atypical depression and double depression predict new-onset cardiovascular disease in U.S. adults (2017)

Adults with atypical MDD or double depression may be subgroups of the depressed population at particularly high risk of new-onset CVD. Thus, these subgroups may (a) be driving the overall depression-CVD relationship and (b) be in need of earlier and/or more intense CVD primary prevention efforts to reduce their excess CVD burden.
Rapid formation of the immune complexes on solid phase in the immune complex transfer enzyme immunoassay for HIV-1 p24 antigen and antibody IgGs to HIV-1 (1998)

In order to perform the immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody IgGs to HIV-1 p17, reverse transcriptase and gp41 antigens as rapidly as possible, methods for rapid formation of the immune complexes on solid phase are described. HIV-1 p24 antigen was reacted with monoclonal anti-p24 Fab'-beta-D-galactosidase conjugate at a high concentration and subsequently with polystyrene beads coated successively with affinity-purified (anti-2,4-dinitrophenyl group) IgG and 2,4-dinitrophenyl-biotinyl bovine serum albumin-affinity-purified anti-p24 Fab' conjugate. Antibody IgGs to HIV-1 were reacted with polystyrene beads coated successively with affinity-purified (anti-2,4-dinitrophenyl group) IgG and 2,4-dinitrophenyl-HIV-1 antigen conjugates and subsequently with HIV-1 antigen-beta-D-galactosidase conjugates. The periods of time used for the formation of the immune complexes comprising the three components on the polystyrene beads (15-30 min) were much shorter than those used in the previous immune complex transfer enzyme immunoassays (90-300 min), and the sensitivities of the present and previous immune complex transfer enzyme immunoassays were similar. The detection limit of the HIV-1 p24 antigen by the present and previous methods were similar (3 to 10 zmol/assay).
Idiotype-Specific Transplantation Resistance to MOPC-315: Abrogation by Post-Immunization Thymectomy (1978)

The idiotype-specific myeloma transplantation resistance induced in BALB/c mice by immunization with the DNP-binding IgAlambda2 protein produced by plasmacytoma MOPC-315 is ablated by post-immunization thymectomy. Sham-thymectomy has no effect. The ablative effect of thymectomy is observed is observed in mice challenged subcutaneously with MOPC-315 cells either 3 days after thymectomy, or after a rest period of 44 days after thymectomy. These observations suggest that short-lived, thymic-dependent suppressive factors may play a role in the idiotype-specific myeloma graft resistance.
Asynchronous transfer mode or synchronous transfer mode or both (1991)

Some of the problem areas attendant upon the asynchronous transfer mode (ATM) concept are addressed. Concern is chiefly with the use of small, fixed-length packets and the issue of congestion control. It is argued that broadband ISDN services should not be tied down to ATM as presently standardized, because ATM has several problems when embedded in a wide area network with diverse services.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Comparative Study of Biosynthetic Human Growth Hormone Immunogenicity in Growth Hormone Deficient Children (1991)

The immunogenicities of six recombinant human growth hormone (rhGH) preparations, from KABI (A rhGH191 and B rhGH192), Eli Lilly (C), Nordisk (D), Sanofi (E) and Serono (F), used to treat 260 GH-deficient children, have been compared using a common specific and sensitive procedure for antibody determination. For this purpose we developed two immunoassays: a competitive liquid radioimmunoassay using 125I-rhGH, and an immunometric solid enzymoimmunoassay in which the rhGHs were immobilized. Blood samples were collected from the GH-deficient children before treatment and after 3, 6, 9, 12, 18 and 24 months of therapy. Human GH antibodies were detected in children treated with 3 of the 6 rhGH preparations. Seven percent of the patients treated with hormone A, 14% with hormone B and 22% with hormone C formed antibodies against the respective rhGH. Differences in capacity and affinity of the hGH antibodies were observed between these anti-GH-positive groups. They could be divided into 2 groups according to their immunopotency. One group (7, 14 and 6% of the patients treated with hormones A, B and C, respectively) developed anti-hGH antibodies with very low binding capacities (30-100 fmol/ml). The other group (16% of the patients treated with hormone C) developed IgG-type antibodies to hGH with higher binding capacities (200-1,200 fmol/ml) and a measurable binding affinity (Ka = 10(8) M-1). These hGH antibodies partially inhibited the binding of labeled GH to its specific liver membrane receptor. However, because of their low titer, they did not inhibit growth in the treated children.(ABSTRACT TRUNCATED AT 250 WORDS)
Acetyl-CoA Carboxylase α Is Essential to Breast Cancer Cell Survival (2006)

Activation of de novo fatty acid synthesis is a characteristic feature of cancer cells. We have recently described an interaction between acetyl-CoA carboxylase alpha (ACCalpha), a key enzyme in fatty acid synthesis, and BRCA1, which indicates a possible connection between lipid synthesis and genetic factors involved in susceptibility to breast and ovarian cancers. For this reason, we explored the role of ACCalpha in breast cancer cell survival using an RNA interference (RNAi) approach. We show that specific silencing of either the ACCalpha or the fatty acid synthase (FAS) genes in cancer cells results in a major decrease in palmitic acid synthesis. Depletion of the cellular pool of palmitic acid is associated with induction of apoptosis concomitant with the formation of reactive oxygen species (ROS) and mitochondrial impairment. Expression of a small interfering RNA (siRNA)-resistant form of ACCalpha mRNA prevented the effect of ACCalpha-RNAi but failed to prevent the effect of FAS gene silencing. Furthermore, supplementation of the culture medium with palmitate or with the antioxidant vitamin E resulted in the complete rescue of cells from both ACCalpha and FAS siRNA-induced apoptosis. Finally, human mammary epithelial cells are resistant to RNAi against either ACCalpha or FAS. These data confirm the importance of lipogenesis in cancer cell survival and indicate that this pathway represents a key target for antineoplastic therapy that, however, might require specific dietary recommendation for full efficacy.
The Activity of Antiepileptic Drugs as Histone Deacetylase Inhibitors (2004)

We found that in addition to VPA, the newer AED TPM and the major metabolite of LEV, PBA, are able to induce histone hyperacetylation in human cells, although with lower potencies than VPA.
A Neonatal Model of Intravenous Staphylococcus epidermidis Infection in Mice <24 h Old Enables Characterization of Early Innate Immune Responses (2012)

Staphylococcus epidermidis (SE) causes late onset sepsis and significant morbidity in catheterized preterm newborns. Animal models of SE infection are useful in characterizing disease mechanisms and are an important approach to developing improved diagnostics and therapeutics. Current murine models of neonatal bacterial infection employ intraperitoneal or subcutaneous routes at several days of age, and may, therefore, not accurately reflect distinct features of innate immune responses to bacteremia. In this study we developed, validated, and characterized a murine model of intravenous (IV) infection in neonatal mice <24 hours (h) old to describe the early innate immune response to SE. C57BL/6 mice <24 h old were injected IV with 10(6), 10(7), 10(8) colony-forming units (CFU) of SE 1457, a clinical isolate from a central catheter infection. A prospective injection scoring system was developed and validated, with only high quality injections analyzed. Newborn mice were euthanized between 2 and 48 h post-injection and spleen, liver, and blood collected to assess bacterial viability, gene expression, and cytokine production. High quality IV injections demonstrated inoculum-dependent infection of spleen, liver and blood. Within 2 h of injection, SE induced selective transcription of TLR2 and MyD88 in the liver, and increased systemic production of plasma IL-6 and TNF-α. Despite clearance of bacteremia and solid organ infection within 48 h, inoculum-dependent impairment in weight gain was noted. We conclude that a model of IV SE infection in neonatal mice <24 h old is feasible, demonstrating inoculum-dependent infection of solid organs and a pattern of bacteremia, rapid and selective innate immune activation, and impairment of weight gain typical of infected human neonates. This novel model can now be used to characterize immune ontogeny, evaluate infection biomarkers, and assess preventative and therapeutic modalities.
Diet-Dependent Shifts in the Bacterial Population of the Rumen Revealed with Real-Time PCR (2001)

A set of PCR primers was designed and validated for specific detection and quantification of Prevotella ruminicola, Prevotella albensis, Prevotella bryantii, Fibrobacter succinogenes, Selenomonas ruminantium-Mitsuokella multiacida, Streptococcus bovis, Ruminococcus flavefaciens, Ruminobacter amylophilus, Eubacterium ruminantium, Treponema bryantii, Succinivibrio dextrinosolvens, and Anaerovibrio lipolytica. By using these primers and the real-time PCR technique, the corresponding species in the rumens of cows for which the diet was switched from hay to grain were quantitatively monitored. The dynamics of two fibrolytic bacteria, F. succinogenes and R. flavefaciens, were in agreement with those of earlier, culture-based experiments. The quantity of F. succinogenes DNA, predominant in animals on the hay diet, fell 20-fold on the third day of the switch to a grain diet and further declined on day 28, with a 57-fold reduction in DNA. The R. flavefaciens DNA concentration on day 3 declined to approximately 10% of its initial value in animals on the hay diet and remained at this level on day 28. During the transition period (day 3), the quantities of two ruminal prevotella DNAs increased considerably: that of P. ruminicola increased 7-fold and that of P. bryantii increased 263-fold. On day 28, the quantity of P. ruminicola DNA decreased 3-fold, while P. bryantii DNA was still elevated 10-fold in comparison with the level found in animals on the initial hay diet. The DNA specific for another xylanolytic bacterium, E. ruminantium, dropped 14-fold during the diet switch and was maintained at this level on day 28. The concentration of a rumen spirochete, T. bryantii, decreased less profoundly and stabilized with a sevenfold decline by day 28. The variations in A. lipolytica DNA were not statistically significant. After an initial slight increase in S. dextrinosolvens DNA on day 3, this DNA was not detected at the end of the experiment. S. bovis DNA displayed a 67-fold increase during the transition period on day 3. However, on day 28, it actually declined in comparison with the level in animals on the hay ration. The amount of S. ruminantium-M. multiacida DNA also increased eightfold following the diet switch, but stabilized with only a twofold increase on day 28. The real-time PCR technique also uncovered differential amplification of rumen bacterial templates with the set of universal bacterial primers. This observation may explain why some predominant rumen bacteria have not been detected in PCR-generated 16S ribosomal DNA libraries.
Successful imaging of malignant melanoma with technetium-99m-labeled monoclonal antibodies. (1989)

F(ab')2 and Fab fragments of murine monoclonal antibody 9.2.27, that recognizes the 250 kD melanoma-associated antigen, were labeled with 99mTc using the bifunctional chelate method of Fritzberg et al. Twenty-seven (27) patients received, intravenously, 10 mg of either F(ab')2 (8), or the Fab (27), labeled with up to 30 mCi of 99mTc. These doses were preceded by an infusion of cold irrelevant antibody. The average serum T1/2 of the F(ab')2 and the Fab were 11 hr and 2 hr, respectively. Twenty-two percent (22%) of the total injected F(ab')2 dose was excreted in the urine in 20 hr, compared to 55% for the Fab group. Imaging was optimal 6-9 hr postinjection for the Fab patients. No nonspecific uptake in liver, spleen, bone marrow, or lung was observed for either antibody form. Overall, (43/53) 81% of known metastases were seen with visualization of tumors as small as 250 mg and tumor localization as high as 0.03% injected dose/g. Immunoperoxidase staining of freshly-frozen tumor nodules removed 24 hr postinjection confirmed antibody deposition in the tumor. Thirty-six previously unknown ("occult") metastatic sites were detected. To date, 12/36 of these sites have been confirmed. We conclude that 99mTc-labeled antibody to melanoma produces high resolution images with a high sensitivity of detecting metastatic melanoma. The detection of previously unknown sites of disease has proven helpful in directing additional diagnostic studies (i.e., CT) as well as planning of therapeutic options.
Waveguides: characteristic modes of hollow rectangular dielectric waveguides (1976)

The field configurations and propagation constants of the normal modes of a hollow rectangular dielectric waveguide have been determined. In addition, the coupling coefficients of a Gaussian free-space mode into the normal modes of a square guide were calculated. The attenuation of each mode is found to be inversely proportional to the cube of the guide aperture 2alpha and proportional to the square of the free-space wavelength lambda. For a hollow dielectric square guide with 2alpha = 1 mm and lambda = 10.6 microm, an attenuation of 0.140 dB/m is predicted for SiO(2) and 0.032 dB/m for BeO. All modes are found to be hybrid modes, although they very closely approximate linearly polarized TEM modes.
Quorum sensing and biofilm formation in Streptococcal infections (2003)

Members of the bacterial genus Streptococcus are responsible for causing a wide variety of infections in humans. Many Streptococci use quorum-sensing systems to regulate several physiological properties, including the ability to incorporate foreign DNA, tolerate acid, form biofilms, and become virulent. These quorum-sensing systems are primarily made of small soluble signal peptides that are detected by neighboring cells via a histidine kinase/response regulator pair.
Biochemical and Immunological Studies on Two Distinct Ganglioside-Hydrolyzing Sialidases from the Particulate Fraction of Rat Brain1 (1990)

Journal Article Biochemical and Immunological Studies on Two Distinct Ganglioside-Hydrolyzing Sialidases from the Particulate Fraction of Rat Brain Get access Taeko Miyagi, Taeko Miyagi *Biochemistry Laboratory, Research Institute for Tuberculosis and Cancer, Tohoku UniversityAoba-ku, Sendari, Miyagi 980 Search for other works by this author on: Oxford Academic PubMed Google Scholar Junji Sagawa, Junji Sagawa *Biochemistry Laboratory, Research Institute for Tuberculosis and Cancer, Tohoku UniversityAoba-ku, Sendari, Miyagi 980 Search for other works by this author on: Oxford Academic PubMed Google Scholar Kimio Konno, Kimio Konno *Biochemistry Laboratory, Research Institute for Tuberculosis and Cancer, Tohoku UniversityAoba-ku, Sendari, Miyagi 980 Search for other works by this author on: Oxford Academic PubMed Google Scholar Shizuo Handa, Shizuo Handa **Department of Biochemistry, Tokyo Medical and Dental UniversityBunkyo-ku, Tokyo 113 Search for other works by this author on: Oxford Academic PubMed Google Scholar Shigeru Tsuiki Shigeru Tsuiki 2 *Biochemistry Laboratory, Research Institute for Tuberculosis and Cancer, Tohoku UniversityAoba-ku, Sendari, Miyagi 980 To whom correspondence should be addressed. Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 107, Issue 5, May 1990, Pages 787–793, https://doi.org/10.1093/oxfordjournals.jbchem.a123126 Published: 01 May 1990 Article history Received: 26 December 1989 Published: 01 May 1990