gebhart/patrepeval huggingface dataset:
Survey of Trust Models in Different Network Domains (2010)

This paper introduces the security and trust concepts in wireless sensor networks and explains the difference between them, stating that even though both terms are used interchangeably when defining a secure system, they are not the same. The difference between reputation and trust is also explained, highlighting that reputation partially affects trust. A survey of trust and reputation systems in various domains is conducted, with more details given to models in ad-hoc and sensor networks as they are closely related to each other and to our research interests. The methodologies used to model trust and their references are presented. The factors affecting trust updating are summarised and some examples of the systems in which these factors have been implemented are given. The survey states that, even though researchers have started to explore the issue of trust in wireless sensor networks, they are still examining the trust associated with routing messages between nodes (binary events). However, wireless sensor networks are mainly deployed to monitor events and report data, both continuous and discrete. This leads to the development of new trust models addressing the continuous data issue and also to combine the data trust and the communication trust to infer the total trust.
Ehrlichioses in Humans: Epidemiology, Clinical Presentation, Diagnosis, and Treatment (2007)

Human ehrlichioses are emerging tickborne infections. "Human ehrlichiosis" describes infections with at least 5 separate obligate intracellular bacteria in 3 genera in the family Anaplasmataceae. Since 1986, these agents and infections (human monocytic ehrlichiosis [HME], caused by Ehrlichia chaffeensis; human granulocytic anaplasmosis [HGA], caused by Anaplasma phagocytophilum; and human ewingii ehrlichiosis, caused by Ehrlichia ewingii) are the causes of most human ehrlichioses. Their prevalence and incidence are increasing where the appropriate tick vectors are found. The diseases generally present as undifferentiated fever, but thrombocytopenia, leukopenia, and increased serum transaminase activities are important laboratory features. Despite clinical similarities, each disease has unique features: a greater severity and a higher case-fatality rate for HME and a higher prevalence of opportunistic infections for HGA. Once an ehrlichiosis is suspected on historical and clinical grounds, doxycycline treatment should be initiated concurrently with attempts at etiologic confirmation using laboratory methods such as blood smear examination, polymerase chain reaction, culture, and serologic tests.
Prediction of membrane-protein topology from first principles (2008)

The current best membrane-protein topology-prediction methods are typically based on sequence statistics and contain hundreds of parameters that are optimized on known topologies of membrane proteins. However, because the insertion of transmembrane helices into the membrane is the outcome of molecular interactions among protein, lipids and water, it should be possible to predict topology by methods based directly on physical data, as proposed >20 years ago by Kyte and Doolittle. Here, we present two simple topology-prediction methods using a recently published experimental scale of position-specific amino acid contributions to the free energy of membrane insertion that perform on a par with the current best statistics-based topology predictors. This result suggests that prediction of membrane-protein topology and structure directly from first principles is an attainable goal, given the recently improved understanding of peptide recognition by the translocon.
ROLE OF POLY (ADP-RIBOSE) SYNTHETASE IN SPLANCHNIC ARTERY OCCLUSION SHOCK IN THE RAT (1997)

Cuzzocrea, Salvatore; Zingarelli, Basilia; Salzman, Andrew L.; Szabó, Csaba Author Information
Attention-Sensitive Alerting (2013)

We introduce utility-directed procedures for mediating the flow of potentially distracting alerts and communications to computer users. We present models and inference procedures that balance the context-sensitive costs of deferring alerts with the cost of interruption. We describe the challenge of reasoning about such costs under uncertainty via an analysis of user activity and the content of notifications. After introducing principles of attention-sensitive alerting, we focus on the problem of guiding alerts about email messages. We dwell on the problem of inferring the expected criticality of email and discuss work on the Priorities system, centering on prioritizing email by criticality and modulating the communication of notifications to users about the presence and nature of incoming email.
Bootstrapping Trust in Commodity Computers (2010)

Trusting a computer for a security-sensitive task (such as checking email or banking online) requires the user to know something about the computer's state. We examine research on securely capturing a computer's state, and consider the utility of this information both for improving security on the local computer (e.g., to convince the user that her computer is not infected with malware) and for communicating a remote computer's state (e.g., to enable the user to check that a web server will adequately protect her data). Although the recent "Trusted Computing" initiative has drawn both positive and negative attention to this area, we consider the older and broader topic of bootstrapping trust in a computer. We cover issues ranging from the wide collection of secure hardware that can serve as a foundation for trust, to the usability issues that arise when trying to convey computer state information to humans. This approach unifies disparate research efforts and highlights opportunities for additional work that can guide real-world improvements in computer security.
Neuroblastoma × spinal cord (NSC) hybrid cell lines resemble developing motor neurons (1992)

We have developed a series of mouse-mouse neural hybrid cell lines by fusing the aminopterin-sensitive neuroblastoma N18TG2 with motor neuron-enriched embryonic day 12-14 spinal cord cells. Of 30 neuroblastoma-spinal cord (NSC) hybrids displaying a multipolar neuron-like phenotype, 10 express choline acetyltransferase, and 4 induce twitching in cocultured mouse myotubules. NSC-19, NSC-34, and their subclones express additional properties expected of motor neurons, including generation of action potentials, expression of neurofilament triplet proteins, and acetylcholine synthesis, storage, and release. In addition, NSC-34 cells induce acetylcholine receptor clusters on cocultured myotubes, and undergo a vimentin-neurofilament switch with maturation in culture, similar to that occurring in neuronal development. NSC cell lines appear to model selected aspects of motor neuron development in an immortalized clonal system.
Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury (2011)

Complement research experienced a renaissance with the discovery of a third activation route, the lectin pathway. We developed a unique model of total lectin pathway deficiency, a mouse strain lacking mannan-binding lectin-associated serine protease-2 (MASP-2), and analyzed the role of MASP-2 in two models of postischemic reperfusion injury (IRI). In a model of transient myocardial IRI, MASP-2-deficient mice had significantly smaller infarct volumes than their wild-type littermates. Mice deficient in the downstream complement component C4 were not protected, suggesting the existence of a previously undescribed lectin pathway-dependent C4-bypass. Lectin pathway-mediated activation of C3 in the absence of C4 was demonstrated in vitro and shown to require MASP-2, C2, and MASP-1/3. MASP-2 deficiency also protects mice from gastrointestinal IRI, as do mAb-based inhibitors of MASP-2. The therapeutic effects of MASP-2 inhibition in this experimental model suggest the utility of anti-MASP-2 antibody therapy in reperfusion injury and other lectin pathway-mediated disorders.
l-Arginine restores splenocyte functions after trauma and hemorrhage potentially by improving splenic blood flow (1999)

Several studies indicate that immune responses are markedly depressed early after onset of hemorrhage. Decreased organ blood flow has been implicated in the pathophysiology of altered immune responses after trauma-hemorrhage. In this regard, administration of L-arginine has been shown to restore depressed intestinal and hepatic blood flow after trauma-hemorrhage, probably due to provision of substrate for constitutive nitric oxide synthase (cNOS). It remains unknown, however, whether administration of L-arginine also ameliorates depressed splenic blood flow and whether this agent has any salutary effects on depressed splenocyte functions after trauma-hemorrhage. Male rats underwent sham operation or laparotomy and were bled to and maintained at a mean arterial blood pressure of 40 mmHg until 40% of maximum shed blood volume (MBV) was returned as Ringer lactate (RL). Hemorrhaged rats were then resuscitated with RL (4 times MBV over 1 h). During resuscitation, rats received 300 mg/kg L-arginine or saline (vehicle) intravenously; 4 h later, splenic blood flow, splenocyte proliferation, and splenocyte interleukin (IL)-2 and IL-3 were determined. Administration of L-arginine improved depressed splenic blood flow and restored depressed splenocyte functions after trauma-hemorrhage. Therefore, provision of L-arginine during resuscitation after trauma-hemorrhage should be considered a novel and safe approach for improving splenic organ blood flow and depressed splenocyte functions under such conditions.
Novel Rho/MRTF/SRF Inhibitors Block Matrix-stiffness and TGF-β–Induced Fibrogenesis in Human Colonic Myofibroblasts (2013)

Targeting the Rho/MRTF/SRF mechanism with second-generation Rho/MRTF/SRF inhibitors may represent a novel approach to antifibrotic therapeutics.
Forced-air warming and fluid warming minimize core hypothermia during abdominal surgery. (2005)

Active warming with air-forced blanket and fluid warming system provides sufficient heat to prevent hypothermia during abdominal surgery.
Concentrated Dispersions of Equilibrium Protein Nanoclusters That Reversibly Dissociate into Active Monomers (2012)

Stabilizing proteins at high concentration is of broad interest in drug delivery, for treatment of cancer and many other diseases. Herein, we create highly concentrated antibody dispersions (up to 260 mg/mL) comprising dense equilibrium nanoclusters of protein (monoclonal antibody 1B7, polyclonal sheep immunoglobulin G, and bovine serum albumin) molecules which, upon dilution in vitro or administration in vivo, remain conformationally stable and biologically active. The extremely concentrated environment within the nanoclusters (∼700 mg/mL) provides conformational stability to the protein through a novel self-crowding mechanism, as shown by computer simulation, while the primarily repulsive nanocluster interactions result in colloidally stable, transparent dispersions. The nanoclusters are formed by adding trehalose as a cosolute which strengthens the short-ranged attraction between protein molecules. The protein cluster diameter was reversibly tuned from 50 to 300 nm by balancing short-ranged attraction against long-ranged electrostatic repulsion of weakly charged protein at a pH near the isoelectric point. This behavior is described semiquantitatively with a free energy model which includes the fractal dimension of the clusters. Upon dilution of the dispersion in vitro, the clusters rapidly dissociated into fully active protein monomers as shown with biophysical analysis (SEC, DLS, CD, and SDS-PAGE) and sensitive biological assays. Since the concept of forming nanoclusters by tuning colloid interactions is shown to be general, it is likely applicable to a variety of biological therapeutics, mitigating the need to engineer protein stability through amino acid modification. In vivo subcutaneous injection into mice results in indistinguishable pharmacokinetics versus a standard antibody solution. Stable protein dispersions with low viscosities may potentially enable patient self-administration by subcutaneous injection of antibody therapeutics being discovered and developed.
Hepatocellular carcinoma in pregnancy (2012)

Hepatic masses in pregnancy are rare (Athanassiou et al. 1998). However, some are known to behave more aggressively during pregnancy. In particular, liver masses associated with pregnancy represent...
EVOLVING USE OF OKT3 MONOCLONAL ANTIBODY FOR TREATMENT OF RENAL ALLOGRAFT REJECTION (1984)

OKT3, a monoclonal antibody reactive with a surface glycoprotein present on all postthymic T cells, was used to treat the initial acute episode of rejection in 30 recipients of cadaveric donor renal allografts. The first 16 patients received 1-5 mg daily for a period of 10-21 days during which the azathioprine and prednisone dosages were sharply reduced. Circulating T cells were eliminated within minutes after the first OKT3 infusion. T cells reactive with OKT3 remained depressed throughout the period of treatment, although a significant number of cells reactive with other T cells subset reagents became detectable after several days of OKT3 treatment. In all instances, the established rejection episode was reversed in 2-8 days without the addition of other immunosuppressive measures. Recurrent rejection occurred in 12 of 16 patients, but with further conventional immunosuppression, 50% of the renal allografts remain functional 20-44 months after transplantation. Fever, chills, and, in some instances, dyspnea following the first dose of OKT3 were the only side-effects observed. Most patients developed antiidiotypic or antimouse immunoglobulin antibodies without apparent clinical sequelae. In the subsequent 14 patients, modifications in the protocol included a steroid bolus prior to the first OKT3 infusion, limitation of therapy to 10 days, resumption of maintenance levels of azathioprine and prednisone prior to discontinuing OKT3, and addition of 3 i.v. doses of cyclophosphamide at the termination of treatment. Respiratory symptoms after the first infusion of the reagent have been eliminated. Antibody responses to OKT3 have been reduced, occurring in 38% as compared with 73% of patients treated previously. Recurrent rejection episodes observed in 8 of 14 patients have been reversible in all but one case. Allograft survival is 86% at 6-17 months posttransplantation. In the entire series of 30 OKT3-treated patients, only 4 grafts (13%) have been lost because of recurrent episodes of rejection.
An electronic effect on protein structure (2003)

The well-known preference of the peptide bond for the trans conformation has been attributed to steric effects. Here, we show that a proline residue with an N-formyl group (H(i-1)-C'(i-1)=O(i-1)), in which H(i-1) presents less steric hindrance than does O(i-1), likewise prefers a trans conformation. Thus, the preference of the peptide bond for the trans conformation cannot be explained by steric effects alone. Rather, an n --> pi* interaction between the oxygen of the peptide bond (O(i-1)), and the subsequent carbonyl carbon in the polypeptide chain (C'(i)) also contributes to this preference. The O(i-1) and C'(i) distance and O(i-1).C'(i)=O(i) angle are especially favorable for such an n --> pi* interaction in a polyproline II helix. We propose that this electronic effect provides substantial stabilization to this and other elements of protein structure.
The Watershed Transform: Definitions, Algorithms and Parallelization Strategies (2000)

The watershed transform is the method of choice for image segmentation in the field of mathematical morphology. We present a critical review of several definitions of the watershed transform and the associated sequential algorithms, and discuss vario
Analgesic effect of bisphosphonates in mice (2001)

Bisphosphonates are analogues of inorganic pyrophosphate and are inhibitors of bone resorption. Many derivatives have been developed for the treatment of enhanced bone resorption; several reports reveal that treatment with bisphosphonates is able to reduce the pain associated with different painful diseases. This study tested the antinociceptive action of four bisphosphonates, clodronate, alendronate, pamidronate and etidronate, in comparison with that of morphine and acetylsalicylic acid using two algesimetric tests in mice, tail-flick and writhing tests. In the tail-flick test, after intravenous (i.v.) injection, a dose-dependent antinociception was present after pamidronate, clodronate and acetylsalicylic acid whereas etidronate and alendronate produced an analgesic effect only with the highest dose tested. We also studied the central effect of clodronate and pamidronate and, after intracerebroventricular injection, both bisphosphonates showed a dose-dependent antinociceptive effect. In the writhing test clodronate and pamidronate showed a statistically significant antinociceptive action after i.v. and intramuscular administration. To verify if clodronate and pamidronate could modulate the peripheral opioid receptors we evaluated the gastrointestinal transit time in mice, but we did not find any effect on the gastrointestinal motility. These data indicate that clodronate and pamidronate present a central and peripheral antinociceptive effect; however, the main mechanism cannot be determined from the present data. We discuss the possible pharmacological hypothesis to interpret the present results. The findings suggest a pharmacological role of the bisphosphonates in the modulation of antinociception even in acute conditions not related to accelerated osteolytic and inflammatory response, with a possible clinical application to control pain.
Macrophages promote benzopyrene-induced tumor transformation of human bronchial epithelial cells by activation of NF-κB and STAT3 signaling in a bionic airway chip culture and in animal models (2015)

We investigated the role of macrophages in promoting benzopyrene (BaP)-induced malignant transformation of human bronchial epithelial cells using a BaP-induced tumor transformation model with a bionic airway chip in vitro and in animal models. The bionic airway chip culture data showed that macrophages promoted BaP-induced malignant transformation of human bronchial epithelial cells, which was mediated by nuclear factor (NF)-κB and STAT3 pathways to induce cell proliferation, colony formation in chip culture, and tumorigenicity in nude mice. Blockage of interleukin (IL)-6 or tumor necrosis factor (TNF)-α signaling or inhibition of NF-κB, STAT3, or cyclinD1 expression abrogated the effect of macrophages on malignant transformation in the bionic airway chip culture. In vivo, macrophages promoted lung tumorigenesis in a carcinogen-induced animal model. Similarly, blockage of NF-κB, STAT3, or cyclinD1 using siRNA transfection decreased the carcinogen-induced tumorigenesis in rats. We demonstrated that macrophages are critical in promoting lung tumorigenesis and that the macrophage-initiated TNF-α/NF-κB/cyclinD1 and IL-6/STAT3/cyclinD1 pathways are primarily responsible for promoting lung tumorigenesis.
Thermodynamic Control of Small RNA-Mediated Gene Silencing (2012)

Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are key regulators of posttranscriptional gene silencing, which is referred to as RNA interference (RNAi) or RNA silencing. In RNAi, siRNA loaded onto the RNA-induced silencing complex (RISC) downreugulates target gene expression by cleaving mRNA whose sequence is perfectly complementary to the siRNA guide strand. We previously showed that highly functional siRNAs possessed the following characteristics: A or U residues at nucleotide position 1 measured from the 5' terminal, four to seven A/Us in positions 1-7, and G or C residues at position 19. This finding indicated that an RNA strand with a thermodynamically unstable 5' terminal is easily retained in the RISC and functions as a guide strand. In addition, it is clear that unintended genes with complementarities only in the seed region (positions 2-8) are also downregulated by off-target effects. siRNA efficiency is mainly determined by the Watson-Crick base-pairing stability formed between the siRNA seed region and target mRNA. siRNAs with a low seed-target duplex melting temperature (T(m)) have little or no seed-dependent off-target activity. Thus, important parts of the RNA silencing machinery may be regulated by nucleotide base-pairing thermodynamic stability. A mechanistic understanding of thermodynamic control may enable an efficient target gene-specific RNAi for functional genomics and safe therapeutic applications.
Enhancing DNA Triple Helix Stability at Neutral pH by the Use of Oligonucleotides Containing a More Basic Deoxycytidine Analog (1996)

DNA triple helices in the parallel binding motif can efficiently be stabilized at neutral pH by replacing deoxycytidine in the third strand by the C-nucleoside 1. The increase in stability is due to the higher pKa of the base in 1, rendering its protonation, required for base pair formation, more favorable at physiological pH.
Diagnostic methods for sputtering plasmas (1978)

This paper reviews the parameters that can be used to characterize glow discharge plasmas of the type used in many sputtering devices, and the methods available to measure those parameters. The review is also generally applicable to the plasmas used in ion plating and plasma-activated reactive evaporation. The objective is to provide a brief overview and a guide to the literature for thin-film specialists. The emphasis is on negative glow-type plasmas, and the diagnostic techniques of optical spectroscopy and electrostatic probes, since these methods are particularly effective for such plasmas and are relatively easy to implement in the laboratory. Guidelines are presented for a corona interpretation of absolute intensity line emission measurements in terms of the density of energetic electrons in an Ar-negative glow. Cases are described in which electrostatic probe measurements were used to investigate performance characteristics of cylindrical-post and cylindrical-hollow magnetron sputtering sources.
Diadermatic Dose Forms Of Testosterone: In-Vitro Release Studies and in-Vivo Absorption In A Human Male (1989)

AbstractThe study was undertaken to evaluate the in-vitro release of testosterone from various topical bases and to screen formulations with the optimum drug release for in-vivo evaluations in a human male. Several diadermatic bases containing 2% testosterone were prepared and studied for the in-vitro release of drug through the cellulose membrane using the diffusion methods. Also, additive ingredients such as, ethanol, polyethylene glycol-400, and dimethylsulfoxide (DMSO) at various concentration levels were included in these formulations to study their effects on the release rate of the drug. The general rank order of testosterone release from these bases was: water-washable base > hydrophilic base > University of California Hospital Base > gel base > cream base > water-soluble base > emulsion base. Except for the water-washable base containing 15% polyethylene glycol-400, all additives had little or no effect on the drug release. The formulations with the best in-vitro drug release were selected and evaluated for in-vivo drug release in a single male volunteer. Each formulation equal to 50mg of testosterone was applied on the forearm, and following each application, 24 hour urine samples were collected and analyzed for the Urinary free testosterone and its principal metabolites, i.e. androsterone and etiocholanolone. A 50mg of oral testosterone in a plain gelatin capsule was used as the control to compare the bioavailability profiles. The ratio of urinary-free testosterone to its main metabolites was found to be (1:1) from one of the topical formulations evaluated compared to (1:20) obtained in the case of oral dose of testosterone used as the control. This suggests that more drug was able to escape the pre-systemic inactivation and resulted in the higher level of free testosterone in the body.