gebhart/patrepeval huggingface dataset:
Quartz-crystal microbalance and spectrophotometric assessments of inter-core and inter-shell reactivities in nanoparticle thin film formation and growth (2001)

Spontaneous core–shell and shell–shell reactivities of thiolate-capped nanoparticles are exploited for assembling nanoparticle network thin films via an exchange–crosslinking–precipitation route. Gold nanoparticles of two different core sizes (2 and 5 nm) capped with decanethiolates and alkylthiols of two different functionalities, 1,9-nonanedithiol (NDT) and 11-mercaptoundecanoic acids (MUA), were studied as the assembly components. The film formation and growth involve inter-core covalent Au–thiolate bonding at both ends of NDT, or inter-shell non-covalent hydrogen-bonding at carboxylic acid terminals of MUA shells. The present work focuses on a kinetic assessment of the controlling factors based on UV-Visible spectrophotometric and quartz-crystal microbalance measurements. These measurements probe the evolution of the surface plasmon resonance band of nanoparticles and monitor the mass loading of the assembled films, respectively. Both qualitative and quantitative insights have been obtained for understanding the dependences of exchange, nucleation and crystallization processes on core size and shell structure. The implication of the results for designing nanostructured assemblies using core–shell nanoparticles as building blocks is discussed in terms of binding site, core size and shell structure.
Molecular Regulation of Adipogenesis (2000)

Adipogenesis, or the development of fat cells from preadipocytes, has been one of the most intensely studied models of cellular differentiation. In part this has been because of the availability of in vitro models that faithfully recapitulate most of the critical aspects of fat cell formation in vivo. More recently, studies of adipogenesis have proceeded with the hope that manipulation of this process in humans might one day lead to a reduction in the burden of obesity and diabetes. This review explores some of the highlights of a large and burgeoning literature devoted to understanding adipogenesis at the molecular level. The hormonal and transcriptional control of adipogenesis is reviewed, as well as studies on a less well known type of fat cell, the brown adipocyte. Emphasis is placed, where possible, on in vivo studies with the hope that the results discussed may one day shed light on basic questions of cellular growth and differentiation in addition to possible benefits in human health.
Securing the Next Steps In Signalling (NSIS) protocol suite (2006)

The Next Steps In Signalling (NSIS) protocol suite represents an extensible framework for enabling various signalling applications over IP-based networks. The framework consists of two layers that need different types of security protection; the lower layer mainly deals with the discovery of adjacent peers and establishment of channel security to protect the delivery of signalling messages between two peers, while the upper layer provides the signalling application specific functionalities. Different security properties are required at the two layers with stronger authorisation functionality at the signalling application layer. In this paper we examine how various security vulnerabilities can be utilised by an adversary, including eavesdropping, Man-In-The-Middle (MITM) attacks, fraud and Denial of Service (DoS) attacks. Moreover, we describe how to protect against a number of selected security threats and highlight some security challenges that require further research.
B-Alkyl Suzuki−Miyaura Cross-Coupling Reactions with Air-Stable Potassium Alkyltrifluoroborates (2003)

The palladium-catalyzed cross-coupling reaction of substituted potassium alkyltrifluoroborates with aryl halides and aryl triflates proceeds readily with moderate to good yields. The potassium alkyltrifluoroborates 1, 2, and 3a−e were easily synthesized and obtained as air-stable crystalline solids that can be stored for long periods of time. All of the cross-couplings proceed under the same reaction conditions using PdCl2(dppf)·CH2Cl2 as catalyst in THF−H2O in the presence of 3 equiv of Cs2CO3 as base.
QCE-6: A Clonal Cell Line with Cardiac Myogenic and Endothelial Cell Potentials (1995)

A clonal cell line (QCE-6) sharing many properties with splanchnic mesodermal cells has been derived from 20-methylcholanthrene-treated cardiogenic mesoderm of the Japanese quail. QCE-6 cells which have been stably maintained for over 4 years in vitro display positive staining with antibodies to cytokeratin, vimentin, cingulin, and N-cadherin but were negative for markers of endothelial, fibroblastic, smooth, and skeletal muscle cell lineages. In the present study, we show that addition of retinoic acid and growth factors initiated cardiomyogenic differentiation in approximately 50% of QCE-6 cells as indicated by the expression of muscle- and cardiac-specific proteins. The distribution of these proteins in induced cells was similar to precontractile cardiac myocytes. In addition to these myogenic cells, cultures of induced QCE-6 cells contained endothelial cells as visualized by the presence of QH1 antigen and extracellular matrix molecules associated with endothelial cells. These data demonstrate that the QCE-6 cell is a progenitor of both cardiac myogenic and endothelial cell lineages and along with other recent studies (Linask and Lash, 1993; Garcia-Martinez and Schoenwolf, 1993) suggest that these two cell lineages may have a common embryonic origin.
Integration of Endothelial Cells in Multicellular Spheroids Prevents Apoptosis and Induces Differentiation (1998)

Single endothelial cells (EC) seeded in suspension culture rapidly undergo apoptosis. Addition of survival factors, such as VEGF and FGF-2, does not prevent apoptosis of suspended EC. However, when cells are allowed to establish cell-cell contacts, they become responsive to the activities of survival factors. These observations have led to the development of a three-dimensional spheroid model of EC differentiation. EC spheroids remodel over time to establish a differentiated surface layer of EC and a center of unorganized EC that subsequently undergo apoptosis. Surface EC become quiescent, establish firm cell-cell contacts, and can be induced to express differentiation antigens (e.g., induction of CD34 expression by VEGF). In contrast, the unorganized center spheroid cells undergo apoptosis if they are not rescued by survival factors. The responsiveness to the survival factor activities of VEGF and FGF-2 was not dependent on cell shape changes since it was retained after cytochalasin D treatment. Taken together, these findings characterize survival factor requirements of unorganized EC and indicate that polarized surface EC differentiate to become independent of exogenous survival factors. Furthermore, they demonstrate that spheroid cell culture systems are useful not just for the study of tumor cells and embryonic stem cells but also for the analysis of differentiated functions of nontransformed cells.
A new filter design technique for coded excitation systems (1992)

To increase range resolution and produce acceptable range sidelobe levels, filtering techniques rather than direct complex correlation are applied in coded excitation systems. A filter design technique based on both peak sidelobe levels and minimum sidelobe energy criteria is developed. In comparison to a classic inverse filter, this approach reduces sidelobe levels under a prespecified threshold. Further reduction can be achieved by extending the filter length. This technique can be more generally applied to similar signal processing problems. Both the mathematical formulation and simulation results demonstrating the utility of the technique are presented. A discussion of quantization effects is included.
Identification of the angiogenic endothelial-cell growth factor-1/thymidine phosphorylase as a potential target for immunotherapy of cancer (2006)

Characterization of the antigens recognized by tumor-reactive T cells isolated from patients successfully treated with allogeneic HLA-matched hematopoietic stem cell transplantation (SCT) can lead to the identification of clinically relevant target molecules. We isolated tumor-reactive cytotoxic CD8(+) T-cell (CTL) clones from a patient successfully treated with donor lymphocyte infusion for relapsed multiple myeloma after allogeneic HLA-matched SCT. Using cDNA expression cloning, the target molecule of an HLA-B7-restricted CTL clone was identified. The CTL clone recognized a minor histocompatibility antigen produced by a single nucleotide polymorphism (SNP) in the angiogenic endothelial-cell growth factor-1 (ECGF1) gene also known as thymidine phosphorylase. The SNP leads to an Arg-to-His substitution in an alternatively translated peptide that is recognized by the CTL. The ECGF1 gene is predominantly expressed in hematopoietic cells, although low expression can also be detected in other tissues. The patient from whom this CTL clone was isolated had mild graft-versus-host disease despite high numbers of circulating ECGF-1-specific T cells as detected by tetramer staining. Because solid tumors expressing ECGF-1 could also be lysed by the CTL, ECGF-1 is an interesting target for immunotherapy of both hematologic and solid tumors.
Rimcazole (BW 234U), a novel antipsychotic agent whose mechanism of action cannot be explained by a direct blockade of postsynaptic dopaminergic receptors in brain (1986)

Abstract Rimcazole is a novel antipsychotic agent free of extrapyramidal side effects as judged by its profile in animal behavioral models and by results of early clinical trials in acute schizophrenic patients. The present study shows that rimcazole is different biochemically from the typical and atypical neuroleptics. Concentrations of 10 −5 to 10 −4 M are necessary to achieve even weak effects on dopamine‐stimulated adenylate cyclase activity (D 1 receptors), [ 3 H]dopamine binding in mesolimbic and striatal areas of rat brain. Rimcazole's extremely weak potency as a D 2 receptor blocker in brain was readily apparent when compared to several other neuroleptics: It was 160 times weaker than sulpiride, the least potent striatal D 2 receptor blocker studied, and 10,000 times weaker than haloperidol, the most potent. Rimcazole had no effect on many of the enzymes involved in catecholamine synthesis and metabolism in vitro, did not effect the synthesis of [ 3 H]dopamine from [ 3 H]tyrosine in vivo, and did not alter the effects of a dopaminergic agonist and a dopaminergic antagonist on [3H]dopamine synthesis in vivo. It was moderately potent as an inhibitor of serotonin (5‐HT2) receptors but had only weak effects on muscarinic cholinergic, α 1 –, α 2 –, β –adrenergic, benzodiazepine, GABA, adenosine (A1 and A2), glycine, serotonin (5‐HT 1 ), imipramine, neurotensin, and phencyclidine receptors and calcium channels in binding assays. It was a moderately potent, competitive inhibitor of dopamine uptake in vitro and had no pharmacologically relevant effects on the spontaneous release of biogenic amines from isolated nerve endings. These data suggest that if rimcazole is shown to be a clinically effective antipsychotic agent, it will be the first antipsychotic whose mechanism of action cannot be explained by a direct blockade of postsynaptic dopaminergic (D 2 ) receptors in brain.
A Parameter Study of Stuctural Respanse Model in Flexible Pavement Substucture Layers (2003)

Several design methods from overseas are employed without considering different conditions such as material properties, climate, and traffic condition in this country. Therefore, there are limitations in application. Therefore, new pavement analysis system which is able to design a pavement efficiently and economically should be set up. In this study, 243 probable sections are classified depending on values of layer thickness and elastic modulus, and the effect of load types for the probable sections are analyzed. The section showing larger load distribution is chosen for analysis. As a result of sensitivity, a layer thickness has more influence on pavement than an elastic modulus does. The stress distribution of FWD test load is larger than that of circular load. This study compares outputs between nonlinear elastic model and linear elastic model. Based on the result, this study finds nonlinear elastic model considering stress condition in the ground is recommended for subbase.
Changing Epidemiology of Invasive Pneumococcal Disease Among Older Adults in the Era of Pediatric Pneumococcal Conjugate Vaccine (2005)

Our findings indicate that use of conjugate vaccine in children has substantially benefited older adults. However, persons with certain comorbid conditions may benefit less than healthier persons from the indirect effects of the new vaccine.
Immunohistochemical localization of tissue factor pathway inhibitor-2 in human tumor tissue (2003)

The progression of neoplasms is frequently associated with thromboembolic complications. Coagulation proteins, particularly tissue factor (TF), have been shown to play a role in tumor growth and metastatic dissemination. TFPI is the principal inhibitor of TF-dependent pathway of blood coagulation, but previous studies failed to detect antigenic TFPI in cancer tissue. Recently, a second inhibitor of tissue factor dependent pathway, TFPI-2 (also known as placental protein 5 [PP5] or matrix-associated serine protease inhibitor [MSPI]), has been described. Information on the presence of TFPI-2 within the malignant tumor tissue still remains obscure, and thus the aim of this study was to evaluate the expression of TFPI-2 in loco in several different neoplasms. TFPI-2 expression was demonstrated by immunohistochemical procedures in neoplastic cells of laryngeal, breast, gastric, colon, pancreatic, renal and endometrial cancer, as well as glial neoplasms. The intensity of staining was not uniform, with higher intensity in more differentiated tumors. G3 breast, gastric, endometrial and colon cancer cells revealed populations of cells that were either TFPI-2 positive or negative. Gastric and renal cancer tissue exhibited the presence of TFPI-2 in tumor infiltrating macrophages. TFPI-2 was also observed in normal tissue of the breast, stomach, colon and pancreas. These data demonstrate that the expression of TFPI-2 diminishes with an increasing degree of malignancy, which may suggest a role for TFPI-2 in the maintenance of tumor stability and inhibition of the growth of neoplasms.
Mechanism of Protective Effect of Recombinant Human Granulocyte Colony‐Stimulating Factor (rG‐CSF) on Pseudomonas Infection (1991)

Decrease in resistance to systemic Pseudomonas infection in cyclophosphamide (CPA)-induced neutropenic mice was prevented by injections of recombinant human granulocyte colony-stimulating factor (rG-CSF). In order to explore mechanism of the prevention of CPA-induced decrease in the anti-infectious resistance by rG-CSF, CPA-treated and then rG-CSF-injected mice were inoculated i.p. with P. aeruginosa, and growth of the infecting bacteria and infiltration of leukocytes in the peritoneal cavity were determined. In the mice who had received 4 daily s.c. injections of rG-CSF from the day after CPA-injection, a large number of neutrophils were mobilized into the peritoneal cavity in response to the bacterial inoculation and growth of the infecting Pseudomonas in the cavity was markedly inhibited, whereas in CPA-induced neutropenic mice few neutrophils were mobilized and the infecting bacteria proliferated vigorously in the peritoneal cavity. These results suggest that administration of rG-CSF prevents CPA-induced neutropenia and neutrophils circulating at normal level in the number are normally mobilized into the peritoneal cavity in response to Pseudomonas inoculation, and that the mobilized neutrophils inhibit proliferation of the infecting Pseudomonas.
Enhancement of AMPA‐mediated Synaptic Transmission by the Protein Phosphatase Inhibitor Calyculin A in Rat Hippocampal Slices (1993)

Using the phosphatase inhibitor calyculin A, we have examined the influence of phosphorylation on synaptic transmission and plasticity in rat CA1 hippocampal slices. Bath application of 0.5-1 microM of calyculin A resulted in an increase of 42.6 +/- 2.9% in synaptic responses. The effect produced by calyculin A was not accompanied by changes in fibre volley, was not associated with changes in paired-pulse facilitation, and could be reproduced by intracellular injection of the compound, thereby indicating a postsynaptic action. Also, the synaptic enhancement produced by calyculin A was expressed only by potentials mediated by amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, but not by the NMDA responses recorded in the presence of the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and low magnesium. The effect of calyculin A could be prevented by KN-62, an inhibitor of calcium/calmodulin-dependent protein kinase II. Long-term potentiation could still be induced in the presence of calyculin A, but the effect of the compound was slightly reduced on potentiated compared with control pathways. These results indicate that calyculin A can selectively increase the efficacy of AMPA receptor-mediated synaptic transmission at excitatory synapses.
Denervation (ablation) of nerve terminalis in renal arteries: early results of interventional treatment of arterial hypertension in Poland (2013)

In our cohort of patients, intra-arterial renal nerve denervation was not associated with either vascular or renal complications out to six months. Nerve ablation of renal arteries led to significant reduction of mean values of arterial systolic, diastolic BP and significant reduction of pulse pressure. The Polish experience is not significantly different compared to that reported in the Symplicity I and Symplicity II international cohorts. The long term durability of this therapy and its application to earlier stages of hypertension or other disease states will require further investigation.
Antimyeloma efficacy of thalidomide in the SCID-hu model (2002)

To determine the mechanism of thalidomide's antimyeloma efficacy, we studied the drug's activity in our severe combined immunodeficiency-human (SCID-hu) host system for primary human myeloma. In this model, tumor cells interact with the human microenvironment to produce typical myeloma manifestations in the hosts, including stimulation of neoangiogenesis. Because mice are not able to metabolize thalidomide efficiently, SCID-hu mice received implants of fetal human liver fragments under the renal capsule in addition to subcutaneous implants of the fetal human bone. Myeloma cell growth in these mice was similar to their growth in hosts without liver implant, as assessed by change in levels of circulating human immunoglobulins and by histologic examinations. Thalidomide given daily by peritoneal injection significantly inhibited myeloma growth in 7 of 8 experiments, each with myeloma cells from a different patient, in hosts implanted with human liver. In contrast, thalidomide exerted an antimyeloma effect only in 1 of 10 mice without liver implants. Microvessel density in the untreated controls was higher than in thalidomide-responsive hosts but not different from nonresponsive ones. Expression of vascular endothelial growth factor by myeloma cells and by other cells in the human bone, determined immunohistochemically, was not affected by thalidomide treatment in any experiment. Our study suggests that thalidomide metabolism is required for its antimyeloma efficacy. Although response to thalidomide was strongly associated with decreased microvessel density, we were unable to conclude whether reduced microvessel density is a primary result of thalidomide's antiangiogenic activity or is secondary to a lessened tumor burden.
Recombinant Single-Chain Variable Fragment Antibodies Directed againstClostridium difficileToxin B Produced by Use of an Optimized Phage Display System (2003)

Recombinant antibody cloning and phage display technologies were used to produce single-chain antibodies (scFv) against Clostridium difficile toxin B. The starting material was the mouse B cell hybridoma line 5A8, which generates a monoclonal antibody against the toxin. The integrated cloning, screening, and phage display system of Krebber et al. (J. Immunol. Methods 201:35-55, 1997) allowed us to rapidly obtain toxin B-binding scFv sequences derived from the hybridoma cell line. The best candidate scFv sequences, based on preliminary enzyme-linked immunosorbent assay (ELISA) screening data were then subcloned into the compatible expression vector. Recombinant single-chain antibodies were expressed in Escherichia coli. A 29-kDa band was observed on polyacrylamide gel electrophoresis as predicted. The expressed product was characterized by immunoblotting and detection with an anti-FLAG antibody. The toxin B-binding function of the single-chain antibody was shown by a sandwich ELISA. The antibody was highly specific for toxin B and did not cross-react with material isolated from a toxin B-negative C. difficile strain. The sensitivity of the soluble single-chain antibody is significantly higher than the original monoclonal antibody based on ELISA data and could detect a minimum of 10 ng of toxin B/well. Competitive ELISAs established that the affinity of the 5A8 parent antibody and the best representative (clone 10) of the single-chain antibodies were similar and in the range of 10(-8) M. We propose that recombinant antibody technology is a rapid and effective approach to the development of the next generation of immunodiagnostic reagents.
Mutations in the STAT5A Gene Are Associated with Embryonic Survival and Milk Composition in Cattle (2008)

The objective of this study was to investigate the association of the signal transducer and activator of transcription 5A (STAT5A) gene with fertilization rate, embryonic survival, and milk production and composition in cattle. The STAT proteins are transcription factors that are specifically activated to regulate gene transcription when cells encounter cytokines and growth factors. The STAT5A gene is a member of the interferon-tau (IFN-tau) and placental lactogen (PL) signaling pathway, which is involved in both milk production and initiation of pregnancy. Using the DNA-pooling sequencing approach, a total of 12 single nucleotide polymorphisms (SNP) were identified, 1 exonic and 11 intronic. For the study of association of these SNP with embryonic survival, 1,551 embryos were produced in vitro from 160 cows and 3 sires. Significant associations with embryonic survival were found for 7, 5, and 2 SNP for embryos produced from sires 1, 2, and 3 respectively. The association of fertilization rate with STAT5A polymorphisms was evaluated in more than 2,300 oocytes. Significant associations were found for 6, 2, and 2 SNP for sires 1, 2, and 3 respectively. For sire 1, 5 SNP showed significant associations with both embryonic survival and fertilization rate compared with 1 SNP for sires 2 and 3. To determine if embryonic losses had occurred before the blastocyst stage, 145 of the surviving embryos were harvested at d 7 of development and genotyped for the single exonic SNP12195. A significant segregation distortion was observed between oocytes produced from 2 sires carrying the same genotype. Thus, it is most likely that STAT5A is associated with 2 mechanisms of embryo death. One is a prefertilization mechanism involving sperm factors that cause low fertilization rate. The second is a postfertilization mechanism that causes incompatibility between the male pronucleus and the oocyte, which in turn leads to death of the embryo before the blastocyst stage. Association testing of SNP12195 (exon 8) and SNP14217 (intron 9) with milk composition revealed that allele G of SNP12195 was associated with a decrease in both protein and fat percentages. However, SNP14217 in intron 9 showed no significant association with milk production or health traits. The G allele of SNP12195 was also associated with low embryonic survival, making this SNP an attractive candidate for progeny testing programs in dairy cattle.