gebhart/patrepeval huggingface dataset:
A correlation of erythrokinetics, ineffective erythropoiesis, and erythroid precursor apoptosis in Thai patients with thalassemia (2000)

The variety of patients with thalassemia in Thailand offers an opportunity to fully characterize the kinetic causes of the anemia and to study apoptosis of marrow erythroid precursors as a possible factor contributing to its severity. Kinetic studies showed that in hemoglobin H (HbH) disease, the extent of hemolysis, as well as the minimally ineffective erythropoiesis, usually falls within the compensatory capacity of normal erythropoiesis; therefore, anemia in patients with HbH partly represents a failure to expand erythropoiesis adequately. Hemoglobin Constant Spring (HbCS), a common variant of alpha thalassemia in Bangkok, causes more severe hemolysis and a distinct increase in ineffective erythropoiesis. Ineffective erythropoiesis plays a much more prominent role in beta thalassemia/hemoglobin E (beta-thal/HbE) disease, in which the variability of the anemia is puzzling. We compared mild and severe cases and found that patients with severe disease had a maximal marrow erythropoietic response that failed to compensate for very short survival of red blood cells and a marked quantitative increase in ineffective erythropoiesis. Analysis of apoptosis of marrow erythroid precursors done both on shipped samples and in Bangkok showed a moderate increase in HbH disease, consistent with the small increase in ineffective erythropoiesis. In patients with homozygous HbCS, there was a further increase in apoptosis, consistent with the additional increase in ineffective erythropoiesis. Patients with beta-thal/HbE disease had the most ineffective erythropoiesis and the most erythroid apoptosis. Thus, it appears that alpha-chain deposition in erythroid precursors, either alpha(A) or alpha(cs), leads to accelerated apoptosis and ineffective erythropoiesis.
Electric and magnetic stimulation of human motor cortex: surface EMG and single motor unit responses. (1989)

1. The effects of different forms of brain stimulation on the discharge pattern of single motor units were examined using the post-stimulus time histogram (PSTH) technique and by recording the compound surface electromyographic (EMG) responses in the first dorsal interosseous (FDI) muscle. Electrical and magnetic methods were used to stimulate the brain through the intact scalp of seven normal subjects. Electrical stimuli were applied either with the anode over the lateral central scalp and the cathode at the vertex (anodal stimulation) or with the anode at the vertex and the cathode lateral (cathodal stimulation). Magnetic stimulation used a 9 cm diameter coil centred at the vertex; current in the coil flowed either clockwise or anticlockwise when viewed from above. 2. Supramotor threshold stimuli produced one or more narrow (less than 2 ms) peaks of increased firing in the PSTH of all thirty-two units studied. Anodal stimulation always produced an early peak. The latencies of the peaks produced by other forms of stimulation, or by high intensities of anodal stimulation, were grouped into four time bands relative to this early peak, at intervals of -0.5 to 0.5, 1-2, 2.5-3.5 and 4-5.5 ms later. Peaks occurring within these intervals are referred to as P0 (the earliest anodal), P1, P2 and P3 respectively. 3. At threshold, anodal stimulation evoked only the P0 peak; at higher intensities, the P2 or more commonly the P3 peak also was recruited. The size of the P0 peak appeared to saturate at high intensities. 4. In five of six subjects, cathodal stimulation behaved like anodal stimulation, except that there was a lower threshold for recruitment of the P2 or P3 peak relative to that of the P0 peak. In the other subject, the P3 peak was recruited before the P0 peak. 5. Anticlockwise magnetic [corrected] stimulation, at threshold, often produced several peaks. These always included a P1 peak, and usually a P3 peak. A P0 peak in the PSTH was never produced by an anticlockwise stimulation [corrected] at intensities which we could explore with the technique. 6. Clockwise magnetic [corrected] stimulation never recruited a P1 peak; in most subjects a P3 peak was recruited first and at higher intensities was accompanied by P0 or P2 peaks. 7. On most occasions when more than one peak was observed in a PSTH, the unit fired in only one of the preferred intervals after each shock. However, double firing was seen in five units when high intensities of stimulation were used.(ABSTRACT TRUNCATED AT 400 WORDS)
Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells (1998)

TEK, or TIE-2, is a receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. We generated mAb against the extracellular domain of human TEK protein to elucidate its expression pattern in human hematopoietic cells. Flow cytometric analysis of bone marrow cells revealed that TEK was expressed in 27% of CD34+ cells, 20% of c-KIT+ cells and 26% of CD34+CD38- cells, indicating that TEK is expressed in a subset of primitive hematopoietic stem cells (HSC). TEK was also expressed in 20% of CD19+ B lymphocytes but not in other lineage-committed cells. Progenitor assays in methylcellulose culture showed that CD34+TEK+ cells formed significantly less BFU-E and CFU-Mix than CD34+TEK- cells, but there was no difference in the number of CFU-GM between these two populations. Two recently identified TEK ligands, termed Angiopoietin-1 and -2, bound to TEK with similar affinities, and Angiopoietin-1 effectively induced TEK phosphorylation in hematopoietic cells. Angiopoietin-2 also induced a low level of TEK phosphorylation and weakened the phosphorylation induced by Angiopoietin-1, suggestive of an elaborate regulator of the TEK-TEK ligand signaling pathway. Although neither ligands affected the proliferation of TEK-transfected hematopoietic cells or the colony formation of CD34+TEK+ bone marrow cells, both promoted the adhesion of TEK-transfected hematopoietic cells to a collagen matrix or a layer of bone marrow stromal cells. These findings indicate that the TEK-TEK ligand signaling pathway is regulated in a refined manner and is involved in hematopoietic cell-microenvironment interaction.
Intelligent talk-and-touch interfaces using multi-modal semantic grammars (1995)

Multi-modal interfaces have been proposed as a way to capture the ease and expressivity of natural communication. Interfaces of this sort allow users to communicate with computers through combinations of speech, gesture, touch, expression, etc. A critical problem in developing such an interface is integrating these different inputs (e.g., spoken sentences, pointing gestures, etc.) into a single interpretation. For example, in combining speech and gesture, a system must relate each gesture to the appropriate part of the sentence. We are investigating this problem as it arises in our talk and touch interfaces, which combine full-sentence speech and screen-touching. Our solution, which has been implemented in two completed prototypes, uses multi-modal semantic grammars to match screen touches to speech utterances. Through this mechanism, our systems can easily support wide variations in the speech patterns used to indicate touch references. Additionally, they can ask specific focused questions to the user in the event of an inability to understand the input. They earl also incorporate other semantic information, such as contextual references or references to previous sentence referents, through this single unified approach. Our two prototypes appear effective in providing a straightforward and powerful interface to novice computer users. 1. Talk and touch interfaces Natural human dialogue is a lot more than sequences of words. Context, gestures, expressions, and implicit knowledge all play a considerable role in conveying complex thoughts and ideas in a discussion. These factors clarify the language, fill in the gaps, and disambiguate the spoken words themselves.
Immunostimulatory oligonucleotides block allergic airway inflammation by inhibiting Th2 cell activation and IgE-mediated cytokine induction (2005)

A single treatment with a CpG-containing immunostimulatory DNA sequence (ISS) given before allergen challenge can inhibit T helper type 2 cell (Th2)-mediated airway responses in animal models of allergic asthma; however, the mechanism of this inhibition remains largely undefined. Here, we demonstrate that airway delivery of ISS before allergen challenge in Th2-primed mice acts in two distinct ways to prevent the allergic responses to this challenge. The first is to prevent induction of cytokines from allergen-specific Th2 cells, as demonstrated by the nearly complete inhibition of Th2 cytokine production, Th2-dependent functional responses, and gene induction patterns. ISS inhibits the Th2 response by rendering lung antigen-presenting cells (APCs) unable to effectively present antigen to Th2 cells, but not to Th1 cells. This loss of APC function correlates with a reduced expression of costimulatory molecules, including programmed cell death ligand (PD-L)1, PD-L2, CD40, CD80, CD86, and inducible T cell costimulator, and of major histocompatibility complex class II on CD11c(+ )APCs from the airways of ISS-treated mice. The second important action of ISS is inhibition of immunoglobulin E-dependent release of Th2 cytokines, especially interleukin 4, from basophils and/or mast cells in the airways of Th2-primed mice. Thus, inhibition by ISS of allergic responses can be explained by two novel mechanisms that culminate in the inhibition of the principal sources of type 2 cytokines in the airways.
Radial basis functions for color conversion (2008)

The most difficult and challenging task in color printing is to reduce costs while maintaining superior quality. This dissertation proposes significant enhancements to printer color conversion techniques including accurate nonlinear models that incorporate perceptual color difference metrics, lossless gray component replacement (GCR) transformations, optimized toner saving algorithms and numerical/perceptual based gamut mapping methods. Radial Basis Functions (RBFs) combined with the Lp norm approximation with emphasis on L1, L2, and L∞ were developed for color conversion. The exchange algorithm was employed in the L∞ and L1 approximations with RBFs that satisfy the Haar condition. Both the Barrodale and Phillips (BP) algorithm for solving the dual problem and the Bartels and Conn's (BC) algorithm for solving the primal were extended to multidimensional color conversion. A new approach for lossless GCR was achieved by finding one dimensional color manifolds in the CMIYK color space using multidimensional optimization techniques. We proposed objective functions for toner savings, cost savings, etc., with no quality degradation. The color conversion with the toner/ink limitation problem was solved via both L1 and L∞ approximation algorithms in the neutral and saturated color regions respectively. The L1 algorithm was a modified Barrodale and Roberts (BR) primal algorithm with an added constraint, while the L∞ algorithm was developed based on the BP dual algorithm which extended the three-stage algorithm to a four-stage algorithm. A novel gamut mapping algorithm was developed based on the numerical model guided by a perceptual color difference model. The direction of the gamut mapping is not fixed as in other methods. The algorithm transformed a connected out-of-gamut colors to connected colors around the boundary of the device gamut. The out-of-gamut colors in a small neighborhood vary continuously and smoothly. Our results indicated that the color conversion quality was significantly improved. The lossless GCR algorithm is accurate and efficient. Both the BP and BC algorithms for solving the toner/ink limitation are able to convert colors from CIELab to CMY with any given toner/ink limitation. We foresee this research will have significant impact on the color reproduction industry.
Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein. (1985)

The COOH terminus of the externally disposed variant surface glycoprotein (VSG) of the eukaryotic pathogenic protozoan Trypanosoma brucei strain 427 variant MITat 1.4 (117) is covalently linked to a novel phosphatidylinositol-containing glycolipid. This conclusion is supported by analysis of the products of nitrous acid deamination or Staphylococcus aureus phosphatidylinositol-specific phospholipase C treatment of purified membrane-form VSG. Lysis of trypanosomes is accompanied by release of soluble VSG, catalyzed by activation of an endogenous phospholipase C. The only apparent difference between membrane-form VSG and soluble VSG is the removal of sn-1,2-dimyristylglycerol. The COOH-terminal glycopeptide derived by Pronase digestion of soluble VSG was characterized by chemical modification and digestion with alkaline phosphatase. The results are consistent with the single non-N-acetylated glucosamine residue being the reducing terminus of the oligosaccharide and in a glycosidic linkage to a myo-inositol monophosphate that is probably myo-inositol 1,2-cyclic monophosphate. A partial structure for the VSG COOH-terminal moiety is presented. This structure represents a new type of eukaryotic post-translational protein modification and membrane anchor. We discuss the relevance of this structure to observations that have been made with other eukaryotic membrane proteins.
Current status of interferons in the treatment of cancer. (1992)

Interferons produced by recombinant DNA technology began phase I trials little more than a decade ago. Today interferon alfa-2 is a mainstay in the treatment of hairy cell leukemia, and has demonstrated benefit in the more common chronic myelogenous leukemia. Interferon alfa-2 also has activity in other hematologic malignancies, including indolent non-Hodgkin's lymphomas, cutaneous T-cell lymphomas, T-cell lymphoma, and multiple myeloma, and in solid tumors such as disseminated melanoma, renal cell carcinoma, Kaposi's sarcoma, endocrine pancreatic tumors, and malignant carcinoid tumors. Interferon alfa, beta, and gamma remain under investigation to define potential roles in ovarian, breast, bladder, and cervical carcinomas and gliomas. The greatest value of the interferons will be in prolonging the disease-free interval when used in combination with other treatment modalities, including surgery, radiation, chemotherapy, and other biologic agents.
Real-time scheduling in a generic fault-tolerant architecture (2002)

Previous ultra-dependable real-time computing architectures have been specialised to meet the requirements of a particular application domain. Over the last two years, a consortium of European companies and academic institutions has been investigating the design and development of a Generic Upgradable Architecture for Real-time Dependable Systems (GUARDS). The architecture aims to be tolerant of permanent and temporary, internal and external, physical faults and should provide confinement or tolerance of software design faults. GUARDS critical applications are intended to be replicated across the channels which provide the primary hardware fault containment regions. In this paper, we present our approach to real-time scheduling of the GUARDS architecture. We use an extended response-time analysis to predict the timing properties of replicated real-time transactions. Consideration is also given to the scheduling of the inter-channel communications network.
A Mild Selective Monobromination Reagent System for Alkoxybenzenes; N-Bromosuccinimide–Silica Gel (1989)

Abstract The monobromination of alkoxybenzenes with N-bromosuccinimide catalyzed by silica gel in carbon tetrachloride occurred preferentially at the para-position. m-t-Butylmethoxybenzene yielded 4-bromo-3-t-butyl-1-methoxybenzene with a selectivity of 74% even in the presence of a bulky substituent at the meta-position.
Dual- and Multi-Energy CT: Principles, Technical Approaches, and Clinical Applications (2015)

In x-ray computed tomography (CT), materials having different elemental compositions can be represented by identical pixel values on a CT image (ie, CT numbers), depending on the mass density of the material. Thus, the differentiation and classification of different tissue types and contrast agents can be extremely challenging. In dual-energy CT, an additional attenuation measurement is obtained with a second x-ray spectrum (ie, a second "energy"), allowing the differentiation of multiple materials. Alternatively, this allows quantification of the mass density of two or three materials in a mixture with known elemental composition. Recent advances in the use of energy-resolving, photon-counting detectors for CT imaging suggest the ability to acquire data in multiple energy bins, which is expected to further improve the signal-to-noise ratio for material-specific imaging. In this review, the underlying motivation and physical principles of dual- or multi-energy CT are reviewed and each of the current technical approaches is described. In addition, current and evolving clinical applications are introduced.
Design, Optimization and Comparison of Permanent Magnet Motors for a Low-Speed Direct-Driven Mixer (2004)

Design, optimization and comparison of permanent magnet motors for a low-speed direct-driven mixer
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families (1991)

Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains may be joined by linker sequences rich in proline or hydroxyamino acids or both. Some of the enzymes contain repeated sequences up to 150 amino acids in length. The enzymes can be grouped into families on the basis of sequence similarities between the catalytic domains. There are sequence similarities between the cellulose-binding domains, of which two types have been identified, and also between some domains of unknown function. The beta-1, 4-glycanases appear to have arisen by the shuffling of a relatively small number of progenitor sequences.
Isoelectric focusing and immunofixation of cerebrospinal fluid and serum in multiple sclerosis. (1981)

CSF from 40 MS patients and 30 individuals with other neurologic diseases were analyzed using isoelectric focusing (IEF) in polyacrylamide gel method. The IgG bands were identified by immunofixation using specific goat anti-human IgG serum. CSF from 28 of 40 MS patients (95%) showed 10-15 distinct oligoclonal bands with different isoelectric points and varying intensities. In contrast, CSF from patients with other neurologic diseases showed a number of diffuse IgG bands in IEF after immunofixation. CSF from 30 of 40 MS patients (75%) showed 1-4 oligoclonal IgG bands in agarose gel electrophoresis (PANAGEL), however, the resolution of bands was generally poor. When 34 coded CSF specimens were analyzed in the IEF and immunofixation procedures, 15 of 17 MS patients (88%) showed distinct oligoclonal IgG bands. Thus, IEF in combination with immunofixation is a superior method for detecting oligoclonal bands in CSF and provides a more valuable tool for the diagnosis of MS. The results also showed that a number of distinct individual oligoclonal IgG bands of MS CSF were of either kappa-type or lambda-type, suggesting that these bands are monoclonal. IgG isolated from 12 of 17 MS sera (70%) showed oligoclonal bands in IEF partly similar to those seen in CSF in the pH region 8.5-9.3; whereas, those from other neurologic diseases showed diffuse bands.
Brain damage due to episodic alcohol exposure in vivo and in vitro: furosemide neuroprotection implicates edema‐based mechanism (1998)

Adult rats intubated with a single dose of ethanol (alcohol; approximately 5 g/kg) for 5 to 10 successive days incur neurodegeneration in the entorhinal cortex, dentate gyrus, and olfactory bulbs accompanied by cerebrocortical edema and electrolyte (Na+, K+) accumulation. The brain damage is not lessened by cotreatment with the NMDA receptor antagonist MK-801; also, as reported elsewhere, MK-801 as well as non-NMDA receptor and Ca2+ channel antagonists are not neuroprotective in a similar, but more compressed, intoxication protocol. However, cotreatment with the electrolyte transport inhibitor/diuretic furosemide reduces alcohol-dependent cerebrocortical damage by 75-85% while preventing brain hydration and electrolyte elevations; olfactory bulb neurodegeneration is not attenuated. In parallel in vitro studies, rat organotypic entorhinal/hippocampal slice cultures exposed to alcohol (50-200 mM) 15 h/day for 6 days, mirroring episodic intoxication in vivo, demonstrate concentration-related release of the cytotoxic indicator, lactate dehydrogenase. Analogous to the in vivo findings, furosemide blocks this alcohol-induced in vitro cytotoxicity. Our results showing neuroprotection by furosemide indicate that brain edema and swelling are essential events in the brain damage induced by episodic alcohol exposure. Furosemide and related agents might be useful as neuroprotective agents in alcohol abuse. We suggest that the neurodegeneration is elicited in part by edema-dependent oxidative stress, but the regional selectivity of the damage may be best explained by physical (mechanical) compression of the limbic cortex against the adjacent tympanic bulla and subsequent neuronal cytoskeletal collapse. A scheme for these apparently nonexcitotoxic metabolic and mechanical pathways initiated by repeated alcohol exposure is proposed.
Connection handoff policies for TCP offload network interfaces (2006)

This paper presents three policies for effectively utilizing TCP offload network interfaces that support connection handoff. These policies allow connection handoff to reduce the computation and memory bandwidth requirements for packet processing on the host processor without causing the resource constraints on the network interface to limit overall system performance. First, prioritizing packet processing on the network interface ensures that its TCP processing does not harm performance of the connections on the host operating system. Second, dynamically adapting the number of connections on the network interface to the current load avoids overloading the network interface. Third, the operating system can predict connection lifetimes to select long-lived connections for handoff to better utilize the network interface. The use of the first two policies improves web server throughput by 12--31% over the baseline throughput achieved without offload. The third policy helps improve performance when the network interface can only handle a small number of connections at a time. Furthermore, by using a faster offload processor, offloading can improve server throughput by 33--72%.