gebhart/patrepeval huggingface dataset:
Massetolides A−H, Antimycobacterial Cyclic Depsipeptides Produced by Two Pseudomonads Isolated from Marine Habitats (1997)

Massetolides A-H (1-8), novel cyclic depsipeptides, and the known compound viscosin (9) have been isolated from cultures of two Pseudomonas sp. isolated from a marine alga and a marine tube worm, respectively. Massetolide A (1) and viscosin (9) exhibit in vitro antimicrobial activity against Mycobacterium tuberculosis and Mycobacterium avium-intracellulare. Precursor-directed biosynthesis has been used to generate unnatural massetolides 11-13 incorporating nonprotein amino acids.
Refractive-index sensor based on long-range surface plasmon mode excitation with longperiod waveguide grating (2009)

We propose a refractive-index sensor that operates on the principle of exciting the long-range surface plasmon mode of a metal-coated waveguide with a long-period grating formed in the waveguide, where the wavelength at which the mode excitation occurs serves as a measure of the refractive index of the external medium. We analyze the sensor with a coupled-mode theory and highlight the effects of the waveguide parameters on the loss of the surface plasmon mode and the performance of the sensor. Our results show that the sensor can provide a sharp resonance for high precision measurements and a high sensitivity comparable to that of an optimized bulk prism-based surface plasmon sensor. Our sensor also offers much flexibility in the choice of waveguide parameters for different applications.
Practice Effect of the Wingate Anaerobic Test (2002)

The purpose of this study was to investigate the presence of a practice effect on the Wingate anaerobic test (WAnT). Twenty-five young adult men (mean age = 20 years) performed 2 trials of the WAnT, which were separated by 7 days. Mean peak power (PP) and mean power (MP) for trials I and II were compared using a 1-way repeated measures analysis of variance to determine if a practice effect existed. Mean PP and MP scores were significantly higher (p < 0.025) on trial II (867.64 and 634.68 W for PP and MP, respectively) than on trial I (764.48 and 604.92 W), indicating that a practice effect occurred. Effect size (Cohen's d) for PP and MP was 0.72 and 0.35, indicating a large effect and small effect, respectively. Therefore, at least 1 full administration should be performed prior to a baseline power output measurement.
Eteplirsen for the treatment of Duchenne muscular dystrophy (2013)

Eteplirsen restored dystrophin in the 30 and 50 mg/kg/wk cohorts, and in subsequently treated, placebo-controlled subjects. Duration, more than dose, accounted for dystrophin production, also resulting in ambulation stability. No severe adverse events were encountered.
Technetium: Chemistry and Radiopharmaceutical Applications (2000)

Chemistry: introduction discovery natural occurrence artificial occurrence isotops and isomers some fundamentals of technetium chemistry analytical chemistry uses of Technetium-99 technetium metal binary compounds and oxide halides oxotechnetates complex chemistry of Technetium-99. Radiopharmaceuticals: introduction generation of 99mTc imaging of organs synthesis aspects and requirements kits synthesis, structure, and development summary of 99mTc radiopharmaceuticals. Appendix: acronyms and abbreviations used in Parts A and B.
Excretion of Human β-Endorphin into Culture Medium by Using Outer Membrane Protein F as a Fusion Partner in Recombinant Escherichia coli (2002)

Escherichia coli BL21 strains were found to excrete a large amount of outer membrane protein F (OmpF) into culture medium during high-cell-density cultivation. From this interesting phenomenon, a novel and efficient OmpF fusion system was developed for the excretion of recombinant proteins by E. coli. The ompF gene of E. coli BL21(DE3) was first knocked out by using the red operon of bacteriophage lambda to construct E. coli MBEL-BL101. For the excretion of human beta-endorphin as a model protein, the beta-endorphin gene was fused to the C terminus of the E. coli ompF gene by using a linker containing the Factor Xa recognition site. To develop a fed-batch culture condition that allows efficient production of OmpF-beta-endorphin fusion protein, three different feeding strategies, an exponential feeding strategy and two pH-stat strategies with defined and complex nutrient feeding solutions, were examined. Among these, the pH-stat feeding strategy with the complex nutrient feeding solution resulted in the highest productivity (0.33 g of protein per liter per h). Under this condition, up to 5.6 g of OmpF-beta-endorphin fusion protein per liter was excreted into culture medium. The fusion protein was purified by anion-exchange chromatography and cleaved by Factor Xa to yield beta-endorphin, which was finally purified by reverse-phase chromatography. From 2.7 liters of culture supernatant, 545.4 mg of beta-endorphin was obtained.
Raman amplification at 1.118 μm in single-mode fibre and its limitation by Brillouin scattering (1982)

A forward Raman gain of 2.9 dB has been measured in a 1.1 km low-loss fibre using an LED at 1.118 μm as a signal source and a CW Nd: YAG laser as a pump. Backward Brillouin scattering, which depletes the pump power towards the end of the fibre, is found to enhance the Raman gain at the front end.
Functional expression of the human serotonin 5-HT1A receptor in Escherichia coli. Ligand binding properties and interaction with recombinant G protein alpha-subunits. (1992)

Signaling through serotonin 5-HT1A receptors involves multiple pathways. We have investigated the functional coupling of the human 5-HT1A receptor to different G proteins using an in vitro reconstitution system based on the expression of recombinant receptor (r5-HT1A) and G alpha-subunits (rG alpha) in Escherichia coli. The r5-HT1A receptor was expressed by insertion in a vector allowing its active expression in E. coli inner membranes. Binding of the selective agonist [3H] +/- 8-hydroxy-(2-N-dipropylamine)tetralin ([3H]8-OH-DPAT) to intact bacteria or E. coli membranes was saturable with a KD of approximately 8 nM and an average of 120 sites/bacterium. Binding properties of several serotoninergic ligands to r5-HT1A receptors were comparable with those measured in mammalian cells. Incubation of rG alpha.beta gamma with E. coli membranes resulted in high affinity agonist [3H]8-OH-DPAT binding (KD = 0.7 nM) and titration with a panel of rG alpha subtypes showed the order of potency: rGi alpha-3 greater than rGi alpha-2 greater than rGi alpha-1 much greater than rGo alpha, while rGs alpha appeared incapable of interacting with 5-HT1A receptors. Moreover, agonist-mediated enhancement of [35S]guanosine 5'-O-(3-thiotriphosphate) binding to rGi alpha confirmed the achievement of the functional interaction between receptor and G proteins. Our findings are in agreement with the in vivo ability of 5-HT1A receptors to activate Gi alpha related to adenylyl cyclase inhibition or K+ channel activation, but do not support previously reported adenylyl cyclase stimulation through interaction with Gs alpha.
The effects of GABAB agonists and gabapentin on mechanical hyperalgesia in models of neuropathic and inflammatory pain in the rat (2001)

We have examined the effects of a novel GABA(B) agonist, CGP35024, in models of chronic neuropathic (partial sciatic ligation) and inflammatory (Freund's complete adjuvant) pain in the rat, and its inhibitory action on spinal transmission in vitro. The effects of CGP35024 were compared with L-baclofen and gabapentin. CGP35024 and L-baclofen reversed neuropathic mechanical hyperalgesia following single subcutaneous or intrathecal administration, but did not affect inflammatory mechanical hyperalgesia. Gabapentin only moderately affected neuropathic hyperalgesia following a single administration by either route, but produced significant reversal following daily administration for 5 days. It was only weakly active against inflammatory hyperalgesia following single or repeated administration. The antihyperalgesic effects of L-baclofen and CGP35024, but not gabapentin, were blocked by the selective GABA(B) receptor antagonist CGP56433A. CGP35024 was seven times more potent against neuropathic hyperalgesia than in the rotarod test for motor co-ordination, whilst L-baclofen was approximately equipotent in the two tests. In the isolated hemisected spinal cord from the rat, CGP35024, L-baclofen and gabapentin all inhibited capsaicin-evoked ventral root potentials (VRPs). CGP35024 and L-baclofen, but not gabapentin, also inhibited the polysynaptic and monosynaptic phases of electrically-evoked VRPs, as well as the 'wind-up' response to repetitive stimulation. These data indicate that CGP35024 and L-baclofen modulate nociceptive transmission in the spinal cord to inhibit neuropathic hyperalgesia, and that CGP35024 has a therapeutic window for antihyperalgesia over spasmolysis.
Pulse shaping and coherent Raman spectroscopy in condensed phases (1991)

A microscopic theory for ultrafast coherent Raman spectroscopy of polyatomic molecules in condensed phases is developed. For off resonant excitation, an effective Hamiltonian that controls the molecular dynamics in a Raman process is derived. The limitations of the driven oscillator model are clarified, and generalized equations of motion, which hold for resonant and off resonant excitation, are derived. Spectral selectivity using pulse shaping is discussed.
Quantum Information Processing and Quantum Error Correction: An Engineering Approach (2012)

Quantum Information Processing and Quantum Error Correction is a self-contained, tutorial-based introduction to quantum information, quantum computation, and quantum error-correction. Assuming no knowledge of quantum mechanics and written at an intuitive level suitable for the engineer, the book gives all the essential principles needed to design and implement quantum electronic and photonic circuits. Numerous examples from a wide area of application are given to show how the principles can be implemented in practice. This book is ideal for the electronics, photonics and computer engineer
Isolation of Pluripotent Stem Cells from Cultured Porcine Primordial Germ Cells1 (1997)

Embryonic germ (EG) cells are undifferentiated stem cells isolated from cultured primordial germ cells (PGC). To date, EG cells have been isolated only in the mouse. Murine EG cells share several characteristics with embryonic stem (ES) cells, including morphology, pluripotency, and the capacity for germline transmission. We report here the isolation of porcine EG cells. PGC collected from Day 24 or 25 porcine embryos were cultured on mitotically inactivated murine fibroblasts. Four EG cell lines were isolated from repeated subculture of porcine PGC. Porcine EG cells morphologically resembled murine ES cells and consistently expressed alkaline phosphatase activity. These cell lines maintained a normal diploid karyotype and survived after cryopreservation. Porcine EG cells were capable of differentiating into a wide range of cell types in culture, including endodermal, trophoblast-like, epithelial-like, fibroblast-like, and neuron-like cells. In suspension culture, porcine EG cells formed embryoid bodies. When injected into host blastocysts, the EG cells were able to differentiate and contribute to tissues of a chimeric piglet. Both in vitro and in vivo evidence demonstrates that the isolated EG cells were pluripotent. These cells are potentially useful for genetic manipulation in pigs.
Dual Roles of Immune Cells and Their Factors in Cancer Development and Progression (2011)

Traditional wisdom holds that intact immune responses, such as immune surveillance or immunoediting, are required for preventing and inhibiting tumor development; but recent evidence has also indicated that unresolved immune responses, such as chronic inflammation, can promote the growth and progression of cancer. Within the immune system, cytotoxic CD8(+) and CD4(+) Th1 T cells, along with their characteristically produced cytokine IFN-γ, function as the major anti-tumor immune effector cells, whereas tumor associated macrophages (TAM) or myeloid-derived suppressive cells (MDSC) and their derived cytokines IL-6, TNF, IL-1β and IL-23 are generally recognized as dominant tumor-promoting forces. However, the roles played by Th17 cells, CD4(+) CD25(+) Foxp3(+) regulatory T lymphocytes and immunoregulatory cytokines such as TGF-β in tumor development and survival remain elusive. These immune cells and the cellular factors produced from them, including both immunosuppressive and inflammatory cytokines, play dual roles in promoting or discouraging cancer development, and their ultimate role in cancer progression may rely heavily on the tumor microenvironment and the events leading to initial propagation of carcinogenesis.
Solubilites of Some Calcium Minerals And Prepared Calcuim Compounds in EDTA (Ethylene Diamine, Tetraacetic Acid) Solutions (1976)

In these studies, the solubilities of calcium minerals such as ulexite, colemanite, pandermite, gypsum, calcite, aragonite and marble and also, phosphate and oxalate compounds of calcium, in 0.1 and 0.2 N aqueous solutions of disodyum EDTA salt, were examined. The solubility studies were made at room temperature under constant mixing. During the studies, the calcination products, between 50-900°C, and original forms of the afore-mentioned calcium compounds were used. As a result of the studies it was seen that carbonate and borate minerals were completely dissociated and gypsum, phosphate and oxalate salts were partially dissociated, depending on the conditions. It was also put forward that the solubility occurred according to the principle of complex formation. Further, by means of these solubility studies it was once more proved that Ca-carbonate minerals and precipitated CaCO3 were thermally decomposed during calcination over 650°C. Calcination and solubility studies on solid CaC2O4 salt showed that this salt was decomposed as CaC2O4 CaCO3 CaO in detail. As a result, the reality that the lattice energies of the compounds and the minerals which were examined in this study were overcome by the EDTA, and the EDTA's chelate-forming force was much more than dipole force of water, was put forward.
MECHANISM OF HARD-TO-COOK DEFECT IN COWPEAS: VERIFICATION VIA MICROSTRUCTURE EXAMINATION (1993)

Introduction It has been hypothesized that part of the hard-tocook (HTC) defect in cowpeas is due to decreases in solubilit y and thermal stability of intracellular proteins during storage since coagulated proteins would limit water to starch and prevent full swelling during cooking. To verify this hypothesis, effects of soaking and heating temperature on texture, water absorption, protein water extractability and microstructure of control and HTC cowpea seeds (aged or treated) were studied. Scanning electron microscopy showed no structural difference between dry control and dry aged seeds. However, after soaking, in contrast to the control, aged seeds exhibited a coarse protein matrix with tightly embedded starch granules and res istance to fracture. Treated seeds also showed ti ght embedment of starch granules. This indi cates that storage proteins had coagulated or aggregated during aging or treatments , as evidenced also by ve ry low extractability. Un like 60 °C, heating at 85 °C dramaticall y decreased protein extractability and led starch granules to swell partially in con trol seeds and little in HTC seeds. When cooked (IOO ' C), HTC seeds showed lack of cell separation and restricted starch swelling, all of which were in sharp contrast with the control. Results not only verified the aforementioned hypothesis but also reaffirmed the role of cell middle lamella, implying involvements of multiple mechanisms in bean hardening.
Wet anisotropic etching for fluidic 1D nanochannels (2003)

In this paper a method is proposed to fabricate channels for fluidic applications with a depth in the nanometer range. Channels with smooth and straight sidewalls are constructed with the help of micromachining technology by etching shallow trenches into 110 silicon using native oxide as a mask material and OPD resist developer as the etchant. Sub-50 nm deep fluidic channels are formed after bonding the nanopatterned wafers with silicon or borofloat-glass wafers. The nanofabrication process is significantly simplified by using native oxide as the main mask material. The etch depth of the nanochannels is limited by the thickness of the native oxide layer, and by the selectivity of the oxide/silicon etch rate (estimated to be at least 250 for 110 silicon at room temperature).
Mucosal immunization with a bacterial protein antigen genetically coupled to cholera toxin A2/B subunits. (1995)

The generation of secretory IgA Abs for specific immune protection of mucosal surfaces depends on stimulation of the mucosal immune system, but this is not effectively achieved by parenteral or even oral administration of most soluble Ags. To harness the exceptional mucosal immunogenicity of cholera toxin (CT), which is largely attributed to the cell-binding property of its B subunit, for the generation of other oral vaccines, we have genetically replaced the toxic A1 subunit of CT with a 42-kDa segment of a streptococcal protein adhesin. This construct was expressed in Escherichia coli as a chimeric protein that retained the GM1 ganglioside-binding activity of CT subunit B and the antigenicity of the streptococcal adhesin, as shown by GM1-ELISA developed with Abs to the steptococcal segment. The protein composition of chromatographically purified chimeric protein was verified by SDS-PAGE and Western blotting with Abs to both antigenic components of the construct. Peroral administration of this chimeric immunogen in mice elicited high levels of mucosal IgA and serum IgG Abs to the streptococcal adhesin, which persisted for at least 6 mo. This strategy allows the development of similar constructs from other candidate Ags for oral immunization against a variety of mucosally acquired infections.
From Genome to Function (2001)

The recent flood of sequence data and the vision of a complete model of life has created new challenges in biology. First, to find the genes and determine or predict their structures and functions. Then, to understand their biological roles and model the complete cell or organism
 in silico. This essay describes for the non-expert the current approaches to just one part of the larger picture--given a protein sequence or a structure, using a computer, what can we deduce about its function; what approaches are available and how good are they?
Selective increases in antibody isotypes and immunoglobulin G subclass responses to secreted antigens in tuberculosis patients and healthy household contacts of the patients (1995)

Mycobacterium tuberculosis-specific antibodies (immunoglobulin M [IgM], IgE, IgG, and IgG subclasses) were determined in 164 tuberculosis patients (pulmonary involvement, n = 135; lymph node involvement, n = 29), 59 healthy household contacts (HC), and 51 healthy endemic donors (EC) by a quantitative enzyme-linked immunosorbent assay for reactivity with culture filtrate. Among the isotypes, significant differences between tuberculosis patient groups with either pulmonary or lymph node involvement and healthy control groups (HC and EC) were detected only for IgG (P < 0.001) and IgG1 (P < 0.001) antibodies. Pulmonary patients also showed a significant difference with IgM (P < 0.01) and IgE (P < 0.05) antibodies. HC showed elevation of only IgM antibodies compared with EC, indicating that IgM antibodies may be an indicator of recent infection with M. tuberculosis. These results suggest that the switching of IgM antibody response to IgG1 is a critical event in disease progression. Polyclonal IgG1, IgG3, and IgE antibodies also showed significant elevation (P < 0.05) in patients compared with EC. A strong correlation (rho = 0.254; P < 0.003) was observed between M. tuberculosis-specific IgG1 and polyclonal IgG1 in patients, suggesting that activations of antigen-specific and polyclonal antibodies are related events. No correlation was found between IgG1 antibodies and purified protein derivative skin test results. Since IgG1 antibody responses to culture filtrate are present only after disease establishment, IgG1 responses could provide a useful diagnostic marker of disease.
Prosodic cues of spontaneous speech in French. (2005)

Disfluencies, when present in speech signal, can make syntactic parsing difficult. This difficulty is increased when machines are involved in communication and when speech devices rely on automatic speech recognition techniques. In order to improve automatic speech parsing and thus speech comprehension, methods have been proposed to filter disfluencies out from the speech signal. Attempts have been made to use prosodic parameters to improve such a filtering. However, before introducing prosodic parameters into automatic speech recognition processes, it would be useful to investigate whether disfluencies can be characterized in a prosodic way and whether their prosodic cues would be representative enough to be used in automatic systems. The aim of this study was to examine to which extent prosodic parameters would be able to characterize disfluencies in French. Word repetitions, filled and silent pauses and speech repairs were described in a prosodic way using statistical analyses of their prosodic parameters. These analyses allowed simple prosodic rules to be formulated. The efficiency of the prosodic rules was evaluated on the task of filled pauses, word repetitions and hesitation detections. 1.
In vitrocomparison of the antigen-binding and stability properties of the various molecular forms of IgA antibodies assembled and produced in CHO cells (1999)

The hallmark of a mucosal immune response is the production of antigen-specific secretory IgA (S-IgA) antibodies in external secretions. S-IgA consists of ten polypeptides produced in two different cell lineages. The heavy and light chains in plasma cells assemble into IgA, which on association with J chain become polymerized, whereas secretory component (SC) is added during transport across the epithelium. Recombinant chimeric mouse-human monomeric, dimeric, and S-IgA antibodies have been produced in a single CHO cell sequentially transfected with expression vectors carrying three independent selective markers for chimeric heavy and light chains, human J chain, and human SC, respectively. Biochemical characterization of the various molecular forms indicates that the assembly of the various polypeptides resulted in species of the expected size and covalence. All chimeric IgA antibodies retained the antigen-binding capacity of the parent mouse IgA antibody. The resistance of S-IgA to protease-rich intestinal washes was enhanced when compared with dimeric IgA lacking associated SC. Up to 20 micrograms of recombinant S-IgA per 1 x 10(6) cells were recovered in 24 h with the best producing clones. We conclude that CHO cells programmed de novo with four different genetic elements can assemble functional chimeric S-IgA.