gebhart/patrepeval huggingface dataset:
Potential-Dependent Wetting of Electroactive Ferrocene-Terminated Alkanethiolate Monolayers on Gold (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotential-Dependent Wetting of Electroactive Ferrocene-Terminated Alkanethiolate Monolayers on GoldJ. A. M. Sondag-Huethorst and L. G. J. FokkinkCite this: Langmuir 1994, 10, 11, 4380–4387Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November 1994https://pubs.acs.org/doi/10.1021/la00023a074https://doi.org/10.1021/la00023a074research-articleACS PublicationsRequest reuse permissionsArticle Views744Altmetric-Citations87LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Total chemical synthesis of enzymatically active human type II secretory phospholipase A 2 (1997)

Human group II secretory phospholipase A2 (sPLA2) is an enzyme found in the alpha granules of platelets and at inflammatory sites. Although its physiological function is unclear, sPLA2 can inhibit blood coagulation reactions independent of its lipolytic action. To study the molecular basis of PLA2 activities, we developed a total chemical synthesis of sPLA2 by chemical ligation of large unprotected peptides. The synthetic segments PLA2-(1-58)-alphaCOSCH2COOH and PLA2-(59-124) were prepared by stepwise solid-phase peptide synthesis and ligated to yield a peptide bond between Gly58 and Cys59. The 124-residue polypeptide product (mass: 13,920 +/- 2 Da) was folded to yield one major product (mass: 13,905 +/- 1 Da), the loss of 15 +/- 3 Da reflecting the formation of seven disulfide bonds. Circular dichroism studies of synthetic sPLA2 showed alpha-helix, beta-structure, and random coil contents consistent with those found in the crystal structure of sPLA2. Synthetic sPLA2 had kcat and Km values identical to those of recombinant sPLA2 for hydrolysis of 1,2-bis(heptanoylthio)-phosphatidylcholine. Synthetic sPLA2, like recombinant sPLA2, inhibited thrombin generation from prothrombinase complex (factors Xa, V, II, Ca2+, and phospholipids). In the absence of phospholipids, both synthetic and recombinant sPLA2 inhibited by 70% prothrombin activation by factors Xa, Va, and Ca2+. Thus, synthetic sPLA2 is a phospholipid-independent anticoagulant like recombinant or natural sPLA2. This study demonstrates that chemical synthesis of sPLA2 yields a fully active native-like enzyme and offers a straightforward tool to provide sPLA2 analogs for structure-activity studies of anticoagulant, lipolytic, or inflammatory activities.
Identification of C-Met Oncogene as a Broadly Expressed Tumor-Associated Antigen Recognized by Cytotoxic T-Lymphocytes (2004)

Our results demonstrate that c-Met oncogene is a novel tumor rejection antigen recognized by CTL and expressed on a broad variety of epithelial and hematopoietic malignant cells.
Development of High Affinity and High Specificity Inhibitors of Matrix Metalloproteinase 14 through Computational Design and Directed Evolution (2017)

Degradation of the extracellular matrices in the human body is controlled by matrix metalloproteinases (MMPs), a family of more than 20 homologous enzymes. Imbalance in MMP activity can result in many diseases, such as arthritis, cardiovascular diseases, neurological disorders, fibrosis, and cancers. Thus, MMPs present attractive targets for drug design and have been a focus for inhibitor design for as long as 3 decades. Yet, to date, all MMP inhibitors have failed in clinical trials because of their broad activity against numerous MMP family members and the serious side effects of the proposed treatment. In this study, we integrated a computational method and a yeast surface display technique to obtain highly specific inhibitors of MMP-14 by modifying the natural non-specific broad MMP inhibitor protein N-TIMP2 to interact optimally with MMP-14. We identified an N-TIMP2 mutant, with five mutations in its interface, that has an MMP-14 inhibition constant (<i>K<sub>i</sub></i> ) of 0.9 pm, the strongest MMP-14 inhibitor reported so far. Compared with wild-type N-TIMP2, this variant displays ∼900-fold improved affinity toward MMP-14 and up to 16,000-fold greater specificity toward MMP-14 relative to other MMPs. In an <i>in vitro</i> and cell-based model of MMP-dependent breast cancer cellular invasiveness, this N-TIMP2 mutant acted as a functional inhibitor. Thus, our study demonstrates the enormous potential of a combined computational/directed evolution approach to protein engineering. Furthermore, it offers fundamental clues into the molecular basis of MMP regulation by N-TIMP2 and identifies a promising MMP-14 inhibitor as a starting point for the development of protein-based anticancer therapeutics.
Oxidation and dissolution rates of UO2(s) in carbonate-rich solutions under external alpha irradiation and initially reducing conditions (2006)

This paper describes the effects of aqueous carbonate concentrations on the UO 2 oxidation and dissolution rates under an alpha beam irradiation in a cyclotron. As solid samples, UO 2 colloids were synthesized by nano-particle precipitation. The specific surface area obtained is 85.3 m 2 /g. All aqueous solutions were buffered in the pH range 8&lt;pH&lt;9. The observation of particles under TEM and X-ray diffraction showed well-crystallized UO 2 . Molecular radiolysis products such as H 2 O 2 and carbonate degradation products where analysed. Solution analysis of dissolved uranium and of radiolytically generated H 2 O 2 allowed to deduce dissolution rates of UO 2 as well as H 2 O 2 consumption rates (deduced from the H 2 O 2 concentrations in presence of UO 2 colloids and those in irradiated blank solutions). In absence of carbonate, the dissolution rates of UO 2 are much lower than the H 2 O 2 consumption rates. The difference is explained by the oxidation of uranium(IV) at the UO 2 /solution interface ( e.g. formation of U 3 O 7 ). In contrast, in the presence of carbonate (up to 4×10 -2 mol/L), the dissolution rates of UO 2 are similar to H 2 O 2 consumption rates. In this case the oxidized uranium is complexed with carbonate ions resulting in highly soluble species as indicated by the geochemical model PHREEQC calculations [UO 2 (CO 3 ) 3 4 - ]. The high carbonate concentration did not affect the H 2 O 2 formation/consumption rates. Radiolytic decomposition of carbonate ions and formation of organic radiolytic degradation products were negligible. The results indicate that under the present experimental conditions, UO 2 oxidation by H 2 O 2 and UO 2 dissolution are connected as a sequential reaction: for a given H 2 O 2 concentration, UO 2 oxidation occurs at a constant rate. At low carbonate concentration, the dissolution of oxidized UO 2 is slower than the oxidation rate (hence oxidation is not rate limiting), while at high carbonate concentrations all oxidized UO 2 becomes immediately dissolved and UO 2 oxidation becomes rate limiting for UO 2 dissolution. This mechanism is in agreement with that proposed in the Matrix Alteration Model (MAM).
LOXL as a target to increase the elastin content in adult skin: a dill extract induces the LOXL gene expression (2006)

The lysyl oxidases lysyl oxidase (LOX) and lysyl oxidase-like (LOXL) are responsible for elastin cross-linking. It was shown recently that LOXL is essential for the elastic fibres homeostasis and for their maintenance at adult age. We first determined whether or not elastin, LOX and LOXL are less expressed during adulthood. The LOX and LOXL mRNA level, quantified by real-time reverse transcriptase-polymerase chain reaction decreased in adult skin fibroblasts compared with fibroblasts from children. In contrast, the elastin mRNA level remains stable at all ages. The goal of this study was to induce elastogenesis at the adult age. Therefore, both enzymes, and in particular LOXL, of which expression is the most affected by age, could be targeted to induce elastogenesis in adult skin. We screened a library of about 1000 active ingredients to find activators capable to stimulate specifically the LOXL gene expression in adult dermal fibroblasts. The positive effect of selected active ingredients was confirmed on fibroblasts grown on monolayers and on dermal and skin equivalent cultures. One extract, obtained from dill (LYS'LASTINE V, Engelhard, Lyon, France), stimulates the LOXL gene expression in dermal equivalents (+64% increase in the LOXL mRNA level when compared with control). At the same time, the elastin detection is increased in dermal equivalents and under the dermal-epidermal junction of skin equivalents, without increase of the elastin mRNA. In conclusion, LOXL can be considered as a new target to reinduce elastogenesis. Its stimulation by a dill extract is correlated with increased elastin detection, suggesting an increase in elastogenesis efficiency.
New Approaches to Preventing, Diagnosing, and Treating Neonatal Sepsis (2010)

Karen Edmond and Anita Zaidi highlight new approaches that could reduce the burden of neonatal sepsis worldwide.
Gene silencing in Caenorhabditis elegans by transitive RNA interference (2003)

When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). Here, we provide evidence that dsRNA is amplified in Caenorhabditis elegans to ensure a robust RNAi response. Our data suggest a model in which mRNA targeted by RNAi functions as a template for 5' to 3' synthesis of new dsRNA (termed transitive RNAi). Strikingly, the effect is nonautonomous: dsRNA targeted to a gene expressed in one cell type can lead to transitive RNAi-mediated silencing of a second gene expressed in a distinct cell type. These data suggest dsRNA synthesized in vivo can mediate systemic RNAi.
PEAR1 attenuates megakaryopoiesis via control of the PI3K/PTEN pathway (2013)

Platelet endothelial aggregation receptor-1 (PEAR1) participates in platelet aggregation via sustaining αIIbβ3 activation. To investigate the role of PEAR1 in platelet formation, we monitored and manipulated PEAR1 expression in vitro in differentiating human CD34(+) hematopoietic stem cells and in vivo in zebrafish embryos. PEAR1 expression rose during CD34(+) cell differentiation up to megakaryocyte (MK) maturation. Two different lentiviral short hairpin knockdowns of PEAR1 did not affect erythropoiesis in CD34(+) cells, but increased colony-forming unit MK cell numbers twofold vs control in clonogenic assays, without substantially modifying MK maturation. The PEAR1 knockdown resulted in a twofold reduction of the phosphatase and TENsin homolog (PTEN) phosphatase expression and modulated gene expression of several phosphatidylinositol 3-kinase (PI3K)-Akt and Notch pathway genes. In zebrafish, Pear1 expression increased progressively during the first 3 days of embryo development. Both ATG and splice-blocking PEAR1 morpholinos enhanced thrombopoiesis, without affecting erythropoiesis. Western blots of 3-day-old Pear1 knockdown zebrafish revealed elevated Akt phosphorylation, coupled to transcriptional downregulation of the PTEN isoform Ptena. Neutralization by morpholinos of Ptena, but not of Ptenb, phenocopied the Pear1 zebrafish knockdown and triggered enhanced Akt phosphorylation and thrombocyte formation. In summary, this is the first demonstration that PEAR1 influences the PI3K/PTEN pathway, a critical determinant of Akt phosphorylation, itself controlling megakaryopoiesis and thrombopoiesis.
A 2.4GHz Direct Conversion Transmitter for Wimax Applications (2006)

The evolving 802.16 WiMax and WiBro standards offer the possibility of high data rate wireless access for home and business users. The first generation systems are currently being designed and deployed in the licensed frequency bands centered around 2.5GHz and 3.5GHz. The use of high data rate OFDM modulations translates into challenging requirements for the transmitter in terms of spectral quality and EVM. In particular, this calls for low distortion, good signal balance and low phase error. This article focuses on the challenges and advantages associated with implementing a direct conversion transmitter in the Wibro band centered at 2.35GHz. A complete mixed signal implementation that includes a dual DAC, IQ modulator, LO synthesizer, variable gain amplifier and RF power detector is presented along with system-level measured results
Carbon Nanotubes for Biomedical Applications (2005)

Carbon nanotubes (CNTs) have many unique physical, mechanical, and electronic properties. These distinct properties may be exploited such that they can be used for numerous applications ranging from sensors and actuators to composites. As a result, in a very short duration, CNTs appear to have drawn the attention of both the industry and the academia. However, there are certain challenges that need proper attention before the CNT-based devices can be realized on a large scale in the commercial market. In this paper, we report the use of CNTs for biomedical applications. The paper describes the distinct physical, electronic, and mechanical properties of nanotubes. The basics of synthesis and purification of CNTs are also reviewed. The challenges associated with CNTs, which remain to be fully addressed for their maximum utilization for biomedical applications, are discussed.
Total Synthesis of (±)-Calanolide A (1996)

Abstract The total synthesis of (±)-Calanolide A, incorporating a ring-forming sequence different from previous procedures, is described.
Comparative Study of the Hepatoprotective Efficacy of  Artemisia iwayomogi  and  Artemisia capillaris  on Ethanol‐Administered Mice (2011)

We compared the effects of ethylacetate extracts from Artemisia iwayomogi (AIE) and Artemisia capillaris (ACE) on ethanol-induced hepatic injury in mice. Ethanol (25% v/v, 5 g/kg body weight) was orally administered once a day for 6 wk. AIE or ACE was provided in the diet (0.05 g/100 g diet). AIE and ACE did not affect hepatic alcohol dehydrogenase activity but did significantly inhibit cytochrome P450 2E1 activity. Hepatic acetaldehyde dehydrogenase 2 activity significantly increased in the AIE group compared to the control group. AIE caused a significant decrease in plasma acetaldehyde levels and aspartate transaminase and lactate dehydrogenase activities, whereas ACE slightly decreased these values compared to the control. Hepatic catalase activity and glutathione levels were significantly increased by AIE and ACE supplements, whereas glutathione peroxidase activity was higher only in the AIE group compared to the control group. AIE and ACE supplements significantly lowered the plasma cholesterol concentration and increased the HDL-cholesterol/total cholesterol ratio compared to the control group. Compared to the control, both AIE and ACE groups showed a significant decrease in hepatic triglyceride levels and an increase in fecal triglyceride excretion simultaneous with inhibition hepatic activities of fatty acid synthase, phosphatidate phosphohyrolase, fatty acid β-oxidation, and carnitine palmitoyltransferase. AIE significantly lowered hepatic cholesterol levels and increased fecal cholesterol levels compared to the control. These results indicate that AIE and ACE exhibit hepatoprotective and hypolipidemic properties by enhancing hepatic alcohol, antioxidant, and lipid metabolism. AIE seemed to have more potent hepatoprotective effects than ACE.
Premenstrual Syndrome Therapy (1998)

University of Iowa Hospitals and Clinics, Iowa City, Iowa Correspondence: Susan R. Johnson, University of Iowa Hospitals and Clinics, Department of OB-Gyn, 200 Hawkins Drive, Iowa City, IA 52242
General query expansion techniques for spoken document retrieval (1999)

This paper presents some developments in query expansion and document representation of our Spoken Document Retrieval (SDR) system since the 1998 Text REtrieval Conference (TREC-7). We have shown that a modification of the document representation combining several techniques for query expansion can improve Average Precision by 17% relative to a system similar to that which we presented at TREC-7 [1]. These new experiments have also confirmed that the degradation of Average Precision due to a Word Error Rate (WER) of 25% is relatively small (around 2% relative). We hope to repeat these experiments when larger document collections become available to evaluate the scalability of these techniques.
Improvement of macrolide antibiotic-producing streptomycete strains by the regeneration of protoplasts. (1983)

Three macrolide antibiotic-producing strains, the spiramycin producer Streptomyces ambofaciens, the tylosin producer Streptomyces fradiae, and the cirramycin producer Streptomyces cirratus easily formed protoplasts when treated with lysozyme in hypertonic medium. Each type of protoplast was regenerated to a mycelial form at a frequency of 90 to 100% in regeneration medium supplemented with a plasma expander. In the spiramycin producer S. ambofaciens and the tylosin producer S. fradiae, antibiotic productivities of the regenerated progeny were drastically changed. Some regenerated progeny from the three strains gave higher production. One from S. ambofaciens showed about twice the productivity of the original strain and one obtained after two rounds of protoplast regeneration from S. fradiae produced about three times as much as the original strain.
Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance (1998)

The recently cloned NPR1 gene of Arabidopsis thaliana is a key regulator of acquired resistance responses. Upon induction, NPR1 expression is elevated and the NPR1 protein is activated, in turn inducing expression of a battery of downstream pathogenesis-related genes. In this study, we found that NPR1 confers resistance to the pathogens Pseudomonas syringae and Peronospora parasitica in a dosage-dependent fashion. Overexpression of NPR1 leads to enhanced resistance with no obvious detrimental effect on the plants. Thus, for the first time, a single gene is shown to be a workable target for genetic engineering of nonspecific resistance in plants.
Purification and Properties of NADPH-linked Diacetyl Reductase (S-Acetoin Forming) fromBacillus polymyxa (1987)

Journal Article Purification and Properties of NADPH-linked Diacetyl Reductase (S-Acetoin Forming) from Bacillus polymyxa Get access Sadaharu Ui, Sadaharu Ui Department of Fermentation Technology, Yamanashi University, Kofu, Yamanashi 400, Japan Search for other works by this author on: Oxford Academic Google Scholar Takahito Masuda, Takahito Masuda Department of Fermentation Technology, Yamanashi University, Kofu, Yamanashi 400, Japan Search for other works by this author on: Oxford Academic Google Scholar Hiroshi Masuda, Hiroshi Masuda Department of Fermentation Technology, Yamanashi University, Kofu, Yamanashi 400, Japan Search for other works by this author on: Oxford Academic Google Scholar Hiroyuki Muraki Hiroyuki Muraki Department of Fermentation Technology, Yamanashi University, Kofu, Yamanashi 400, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 51, Issue 5, 1 May 1987, Pages 1447–1448, https://doi.org/10.1080/00021369.1987.10868222 Published: 01 May 1987 Article history Received: 11 November 1986 Published: 01 May 1987
Fibre dispersion or pulse spectrum measurement usinga sampling oscilloscope (1997)

A simple technique for measuring optical fibre dispersion (or pulse spectrum if fibre dispersion is known) is demonstrated. Ultra-short pulses are linearly dispersed in the fibre under test, thus mapping their spectrum to the time domain. The delay between two spikes in the dispersed pulse&apos;s temporal profile, corresponding to two spectral spikes, accurately gives the dispersion.
Interleukin 15 Skews Monocyte Differentiation into Dendritic Cells with Features of Langerhans Cells (2001)

Langerhans cells (LCs) represent a subset of immature dendritic cells (DCs) specifically localized in the epidermis and other mucosal epithelia. As surrounding keratinocytes can produce interleukin (IL)-15, a cytokine that utilizes IL-2Rgamma chain, we analyzed whether IL-15 could skew monocyte differentiation into LCs. Monocytes cultured for 6 d with granulocyte/macrophage colony-stimulating factor (GM-CSF) and IL-15 differentiate into CD1a(+)HLA-DR(+)CD14(-)DCs (IL15-DCs). Agents such as lipopolysaccharide (LPS), tumor necrosis factor (TNF)alpha, and CD40L induce maturation of IL15-DCs to CD83(+), DC-LAMP(+) cells. IL15-DCs are potent antigen-presenting cells able to induce the primary (mixed lymphocyte reaction [MLR]) and secondary (recall responses to flu-matrix peptide) immune responses. As opposed to cultures made with GM-CSF/IL-4 (IL4-DCs), a proportion of IL15-DCs expresses LC markers: E-Cadherin, Langerin, and CC chemokine receptor (CCR)6. Accordingly, IL15-DCs, but not IL4-DCs, migrate in response to macrophage inflammatory protein (MIP)-3alpha/CCL20. However, IL15-DCs cannot be qualified as "genuine" Langerhans cells because, despite the presence of the 43-kD Langerin, they do not express bona fide Birbeck granules. Thus, our results demonstrate a novel pathway in monocyte differentiation into dendritic cells.
Glucagon-like peptide-1: a potent regulator of food intake in humans (1999)

Intravenous infusions of GLP-1 decrease spontaneous food intake even at physiological plasma concentrations, implying an important role for GLP-1 in the regulation of the early satiety response in humans.