gebhart/patrepeval huggingface dataset:
In vitro and in vivo evaluation of tiacumicins B and C against Clostridium difficile (1991)

Tiacumicins B and C are members of a novel group of 18-membered macrolide antibiotics with in vitro activity against Clostridium difficile. The MICs against 15 strains of C. difficile were 0.12 to 0.25 microgram/ml for tiacumicin B, 0.25 to 1 microgram/ml for tiacumicin C, and 0.5 to 1 microgram/ml for vancomycin. The resistance frequency for both compounds against C. difficile was less than 2.8 x 10(-8) at four and eight times the MIC. The in vivo activities of the tiacumicins against two strains of C. difficile were compared with that of vancomycin in a hamster model of antibiotic-associated colitis. Oral therapy with 0.2, 1, or 5 mg of tiacumicin B or C per kg of body weight protected 100% of clindamycin-treated hamsters exposed to C. difficile ATCC 9689. Oral treatment with identical doses of vancomycin produced a prolonged, dose-dependent survival of hamsters, but it did not prevent the development of fatal colitis at doses of up to 5 mg/kg. When clindamycin-treated animals were exposed to another strain of C. difficile, both tiacumicin B and vancomycin were protective at 5 mg/kg, but not at lower doses. Tiacumicin C was not tested in vivo against the second strain of C. difficile. No tiacumicin B or C was detected in the sera of hamsters treated with single oral doses of 25 mg/kg, while antibiotic levels in the ceca of these hamsters reached 248 micrograms/ml and 285 mg/ml for tiacumicins B and C, respectively. The tiacumicins demonstrated in vitro and in vivo potencies against C. difficile and achieved high concentrations in the cecum, but not the serum, of hamsters after oral administration.
Location of Bacterial Biofilm in the Mucus Overlying the Adenoid by Light Microscopy (2009)

Bacteria of the adenoid reside in secretions on the surface and in crypts. Biofilms, predominantly planktonic, were present on 8 of 9 adenoids excised because of hypertrophy. Whether biofilms have a role in the causation of adenoid hypertrophy is not known.
New federalism: problems or opportunities for rural America? (2005)

2005 Annual report, Center for the Study of Rural America
Photolabile 1-(2-nitrophenyl)ethyl phosphate esters of adenine nucleotide analogs. Synthesis and mechanism of photolysis (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotolabile 1-(2-nitrophenyl)ethyl phosphate esters of adenine nucleotide analogs. Synthesis and mechanism of photolysisJeffery W. Walker, Gordon P. Reid, James A. McCray, and David R. TrenthamCite this: J. Am. Chem. Soc. 1988, 110, 21, 7170–7177Publication Date (Print):October 1, 1988Publication History Published online1 May 2002Published inissue 1 October 1988https://pubs.acs.org/doi/10.1021/ja00229a036https://doi.org/10.1021/ja00229a036research-articleACS PublicationsRequest reuse permissionsArticle Views2622Altmetric-Citations269LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Anatomic Considerations for Posterior Approach to the Sacroiliac Joint (1996)

This approach facilities improved access to the sacroiliac joint for debridement and arthrodesis with minimal soft tissue dissection and iliac bone resection.
High-gain weakly nonlinear flux-modulated Josephson parametric amplifier using a SQUID array (2014)

We have developed and measured a high-gain quantum-limited microwave parametric amplifier based on a superconducting lumped LC resonator with the inductor L including an array of eight superconducting quantum interference devices (SQUIDs). This amplifier is parametrically pumped by modulating the flux threading the SQUIDs at twice the resonator frequency. Around 5 GHz, a maximum gain of 31 dB, a product amplitude gain $\ifmmode\times\else\texttimes\fi{}$ bandwidth above 60 MHz, and a 1 dB compression point of $\ensuremath{-}123$ dBm at 20 dB gain are obtained in the nondegenerate mode of operation. Phase-sensitive amplification-deamplification is also measured in the degenerate mode and yields a maximum gain of 37 dB. The compression point obtained is 18 dB above what would be obtained with a single SQUID of the same inductance, due to the smaller nonlinearity of the SQUID array.
The Effect of Different Linkers on Target Cell Catabolism and Pharmacokinetics/Pharmacodynamics of Trastuzumab Maytansinoid Conjugates (2012)

Trastuzumab emtansine (T-DM1) is an antibody-drug conjugate consisting of the anti-HER2 antibody trastuzumab linked via a nonreducible thioether linker to the maytansinoid antitubulin agent DM1. T-DM1 has shown favorable safety and efficacy in patients with HER2-positive metastatic breast cancer. In previous animal studies, T-DM1 exhibited better pharmacokinetics (PK) and slightly more efficacy than several disulfide-linked versions. The efficacy findings are unique, as other disulfide-linked antibody-drug conjugates (ADC) have shown greater efficacy than thioether-linked designs. To explore this further, the in vitro and in vivo activity, PK, and target cell activation of T-DM1 and the disulfide-linked T-SPP-DM1 were examined. Both ADCs showed high in vitro potency, with T-DM1 displaying greater potency in two of four breast cancer cell lines. In vitro target cell processing of T-DM1 and T-SPP-DM1 produced lysine-N(ε)-MCC-DM1, and lysine-N(ε)-SPP-DM1 and DM1, respectively; in vivo studies confirmed these results. The in vitro processing rates for the two conjugate to their respective catabolites were similar. In vivo, the potencies of the conjugates were similar, and T-SPP-DM1 had a faster plasma clearance than T-DM1. Slower T-DM1 clearance translated to higher overall tumor concentrations (conjugate plus catabolites), but unexpectedly, similar levels of tumor catabolite. These results indicate that, although the ADC linker can have clear impact on the PK and the chemical nature of the catabolites formed, both linkers seem to offer the same payload delivery to the tumor.
Alcohol dehydrogenases and an alcohol oxidase involved in the assimilation of exogenous fatty alcohols in Yarrowia lipolytica (2015)

The yeast Yarrowia lipolytica can assimilate hydrophobic substrates, including n-alkanes and fatty alcohols. Here, eight alcohol dehydrogenase genes, ADH1-ADH7 and FADH, and a fatty alcohol oxidase gene, FAO1, were analyzed to determine their roles in the metabolism of hydrophobic substrates. A mutant deleted for all of these genes (ALCY02 strain) showed severely defective growth on fatty alcohols, and enhanced sensitivity to fatty alcohols in glucose-containing media. The ALCY02 strain grew normally on n-tetradecane or n-hexadecane, but exhibited slightly defective growth on n-decane or n-dodecane. It accumulated more 1-dodecanol and less dodecanoic acid than the wild-type strain when n-dodecane was fed. Expression of ADH1, ADH3 or FAO1, but not that of other ADH genes or FADH, in the ALCY02 strain restored its growth on fatty alcohols. In addition, a triple deletion mutant of ADH1, ADH3 and FAO1 showed similarly defective growth on fatty alcohols and on n-dodecane to the ALCY02 strain. Microscopic observation suggests that Adh1p and Adh3p are localized in the cytosol and Fao1p is in the peroxisome. These results suggest that Adh1p, Adh3p and Fao1p are responsible for the oxidation of exogenous fatty alcohols but play less prominent roles in the oxidation of fatty alcohols derived from n-alkanes.
mRNA expression of components of the insulin-like growth factor system in breast cancer cell lines, tissues, and metastatic breast cancer cells. (1998)

IGF-1 and 2 are thought to be important growth factors for breast cancer. However, gene expression of IGFs or IGF receptors in breast cancer tissues, and especially in metastatic breast cancer cells, is not well known. Expression of mRNA encoding for IGF-1, IGF-2, IGF-receptor 1 and 2, IGF binding proteins- 1 to -6, insulin receptor and insulin was determined in the NIH MCF-7 breast cancer cell line, in specimens from breast cancer tissues, and in 6 primary breast cancer cell cultures obtained from metastatic breast cancer, using rT-PCR technique. Specific mRNA sequences encoding for IGF-receptor 1 and 2, IGFBP-2, -4 and insulin receptor were identified in all cell cultures and most of the tissue specimens. Though in most of the tissues additional expression of IGF-1 and IGF-2 was detected, there was no mRNA encoding for these proteins in MCF-7 cell cultures as well as in the primary cell cultures of metastatic breast cancers. In none of our specimens mRNA encoding for IGFBP-1, -3, -5, -6 and insulin was detectable. IGF-receptor expression in cancer tissues and metastatic breast cancer cells supports the hypothesis that IGFs increase tumor cell proliferation in vivo. Expression of IGF-1 and IGF-2 in tumor tissues but not in cancer cell cultures indicates an IGF expression located predominantly in stromal parts of cancer tissues.
Toward an understanding of transfusion‐related acute lung injury: statement of a consensus panel (2004)

Peer Reviewed
From uncertainty to visual exploration (1991)

The authors attempt to determine what can be inferred from ambiguity in processes of visual interpretation. They discuss this question in a specific context: the interpretation of scene geometry in the form of parametrized volumetric models. Ambiguity is described as a local probabilistic property of the misfit error surface in the parameter space of superellipsoid models, namely, as an ellipsoid of confidence in which there is a given probability that the true parameters can be found. The authors show how to project the ellipsoid of confidence back into 3D space to obtain the shell in which the true 3D surface most probably lies and introduce what they call the uncertainty as a local property of the fitted model's surface. They propose a technique that can use this information to plan a new direction of view that minimizes the ambiguity of subsequent interpretation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Idiotype specific T-cell lines inducing experimental systemic lupus erythematosus in mice. (1991)

Immunization of mice with either antibodies bearing the 16/6 idiotype (16/6 Id) or anti-idiotypic antibodies against the 16/6 Id induces experimental systemic lupus erythematosus (SLE). We report here the establishment and characterization of 16/6 Id-specific T-cell lines from C3H.SW (H-2b) and BALB/c (H-2d) mice. Both lines proliferate specifically in response to the 16/6 Id in an H-2-restricted manner. The injection of 16/6 Id-specific T cells into syngeneic mice led to the development of experimental SLE. Furthermore, inoculation of the 16/6 Id-specific T-cell line derived from C3H.SW mice into the H-2 compatible C57BL/6 mice, which are non-responders to the 16/6 Id, induced experimental SLE. This report provides direct evidence for the role of idiotype-specific T cells in the induction of experimental SLE.
Pollen- and Anther-Specific chi Promoters from Petunia: Tandem Promoter Regulation of the chiA Gene (1990)

Arjen J. van Tunen, Leon A. Mur, Gabrielle S. Brouns, Jan-Derk Rienstra, Ronald E. Koes, Joseph N. M. Mol, Pollen- and Anther-Specific chi Promoters from Petunia: Tandem Promoter Regulation of the chiA Gene, The Plant Cell, Vol. 2, No. 5 (May, 1990), pp. 393-401
A High-Rate, Nanocomposite LiFePO[sub 4]∕Carbon Cathode (2005)

We describe here a new type of template-prepared nanostructured electrode, a nanocomposite consisting of monodispersed nanofibers of the electrode material mixed with an electronically conductive carbon matrix. This unique nanocomposite morphology allows these electrodes to deliver high capacity, even when discharged at the extreme rates necessary for many pulse-power applications. For example, this nanocomposite electrode delivers almost 100% of its theoretical discharge capacity at the high discharge rate of 3 C, and 36% of its theoretical capacity at the enormous discharge rate of 65 C. This new nanocomposite electrode shows such excellent rate capabilities because the nanofiber morphology mitigates the problem of slow -transport in the solid state, and the conductive carbon matrix overcomes the inherently poor electronic conductivity of .
Effect of β‐Sheet Crystals on the Thermal and Rheological Behavior of Protein‐Based Hydrogels Derived from Gelatin and Silk Fibroin (2005)

Novel protein-based hydrogels have been prepared by blending gelatin (G) with amorphous Bombyx mori silk fibroin (SF) and subsequently promoting the formation of beta-sheet crystals in SF upon exposure to methanol or methanol/water solutions. Differential scanning calorimetry of the resultant hydrogels confirms the presence and thermoreversibility of the G helix-coil transition between ambient and body temperature at high G concentrations. At low G concentrations, this transition is shifted to higher temperatures and becomes progressively less pronounced. Complementary dynamic rheological measurements reveal solid-liquid cross-over at the G helix-coil transition temperature typically between 30 and 36 degrees C in blends prior to the formation of beta-sheet crystals. Introducing the beta-sheet conformation in SF stabilizes the hydrogel network and extends the solid-like behavior of the hydrogels to elevated temperatures beyond body temperature with as little as 10 wt.-% SF. The temperature-dependent elastic modulus across the G helix-coil transition is reversible, indicating that the conformational change in G can be used in stabilized G/SF hydrogels to induce thermally triggered encapsulant release.
Robust and Fast Pattern Matching for Intrusion Detection (2010)

The rule language of an Intrusion Detection System (IDS) plays a critical role in its effectiveness. A rule language must be expressive, in order to describe attack patterns as precisely as possible. It must also allow for a matching algorithm with predictable and low complexity, in order to ensure robustness against denial-of-service attacks. Unfortunately, these requirements often conflict. We show, for instance, that a single rule, when coupled with a backtracking matching algorithm, can bring the processing rate down to nearly ONE packet per second. Performance vulnerabilities of this type are known for patterns described using regular expressions, and can be avoided by using a deterministic matching algorithm. Increasingly, however, rules are being written using the more powerful regex syntax, which includes non-regular features such as back-references. The matching algorithm for general regex's is based on backtracking, and is thus vulnerable to attacks. The main contribution of this paper is a deterministic algorithm for the full regex syntax, which builds upon the deterministic algorithm for regular expressions. We provide a (rough) complexity bound on the worst-case performance, and show that this bound can be tightened through compile-time analysis of the regex structure. These bounds can be used as an admissibility check, to isolate expressions that require further analysis. Finally, we present an implementation of these algorithms in the context of the Snort IDS, and experimental results on several packet traces which show substantial improvement over the backtracking algorithm.
UASrpg can function as a heterochromatin boundary element in yeast (1999)

The HM loci in Saccharomyces cerevisiae constitute region-specific but gene-nonspecific repression domains, as a number of heterologous genes transcribed by RNA polymerase II or III are silenced when placed at these loci. The promoters of the Ashbya gossypii TEF gene and the S. cerevisiae TEF1 and TEF2 genes, however, are resistant to transcriptional silencing by the HM silencers in yeast. Moreover, when interposed between the HML alpha genes and the E silencer, certain segments of these promoters block the repression effect of the silencer on the alpha genes. All of these fragments contain UASrpg (upstream activation sequence of ribosome protein genes) composed of multiple binding sites for Rap1. In fact, a 149-bp segment consisting essentially of only three tandem Rap1-binding sites from the UASrpg of yeast TEF2 exhibits silencer-blocking activity. This element also exhibits insulating activity and orientation dependence characteristic of known chromatin boundary elements. Finally, the element blocks the physical spread of heterochromatin initiated at a silencer. This segment provides the first example of chromatin domain boundary or insulator elements in yeast.
Use of Double-Replacement Gene Targeting to Replace the Murine α-Lactalbumin Gene with Its Human Counterpart in Embryonic Stem Cells and Mice (1994)

The mouse alpha-lactalbumin gene has been replaced with the human gene by two consecutive rounds of gene targeting in hypoxanthine phosphoribosyltransferase (HPRT)-deficient feeder-independent murine embryonic stem (ES) cells. One mouse alpha-lactalbumin allele was first replaced by an HPRT minigene which was in turn replaced by human alpha-lactalbumin. The end result is a clean exchange of defined DNA fragments with no other DNA remaining at the target locus. Targeted ES cells at each stage remained capable of contributing efficiently to the germ line of chimeric animals. Double replacement using HPRT-deficient ES cells and the HPRT selection system is therefore a powerful and flexible method of targeting specific alterations to animal genes. A typical strategy for future use would be to generate a null mutation which could then be used to produce multiple second-step alterations at the same locus.
Mobile in vivo biopsy robot (2006)

A mobile in vivo camera robot was developed to provide the ability for a single port biopsy procedure. Such a robot can be inserted into the abdominal cavity through a standard trocar. The surgeon controls the robot using visual feedback from the on-board camera. Measurements were made to identify the forces required to successfully biopsy in vivo tissue, including clamping and tearing forces. The robot design was developed around these parameters and the need to traverse the abdominal environment using specially designed wheels. This mobility allows the biopsy robot to move to the area of interest to sample specific tissues. The lead-screw linkage system that actuated the graspers allows for large force production through careful mechanical design. In vivo testing of this system in a porcine (pig) model has been successful. The robot is capable of traversing the entire in vivo abdominal environment and has successfully been used to biopsy hepatic tissue. In addition, experimental analysis of the biopsy mechanism shows good results towards more elaborate tissue manipulation in the future
The impact of genetic variation in comt and bdnf on resting‐state functional connectivity (2012)

Abstract Genetic imaging techniques allow investigation of the mechanisms by which genetic variants influence brain structure and function. The default mode network (DMN) is characterized by a default state of neuronal activity in the brain that is linked to core processes of human cognition. This study examined the role of catechol‐ O ‐methyl transferase (COMT) and brain‐derived neurotrophic factor (BDNF) polymorphisms on functional connectivity between brain areas. Twenty‐three healthy volunteers underwent a resting‐state functional magnetic resonance imaging scan and genotyping of COMT and BDNF single nucleotide polymorphisms (SNPs). A resting‐state functional connectivity map was created using the posterior cingulate cortex (PCC) as a seed region. The Val/Val homozygote group of the COMT Val 158 Met SNP showed significantly greater DMN connectivity in the medial and superior frontal gyri and cerebellum compared with the Met allele carrier group. For the BDNF Val 66 Met SNP, connectivity between the PCC and precuneus was stronger in the Val/Val homozygote group than in the Met allele carrier group. Different patterns of DMN connectivity related to BDNF and COMT SNPs were observed in this study. These findings suggest interaction between genes and functional connectivity in the brain and indicate that altered functional connectivity may be an endophenotype of cognitive vulnerability. © 2012 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 22, 97–102, 2012
The use of vasopressin for treating vasodilatory shock and cardiopulmonary arrest (2009)

Vasopressin (0.01-0.04 U/min, IV) should be considered in small animal veterinary patients with vasodilatory shock that is unresponsive to fluid resuscitation and catecholamine (dobutamine, dopamine, and norepinephrine) administration. Vasopressin (0.2-0.8 U/kg, IV once) administration during cardiopulmonary resuscitation in small animal veterinary patients with pulseless electrical activity or ventricular asystole may be beneficial for myocardial and cerebral blood flow.
Automatic generation of microprocessor test programs (1982)

This paper presents an automatic generation system for behavioral test programs of microprocessors. First, the test environment is presented, as well as its consequences on test program generation. In the second part, the test principles are outlined; it is a behavioral test, i.e a test determined from the user's description of the microprocessor; the resulting test programs are quite modular. The third part presents the description language and the generation system.
In Situ Reactivity and TOF-SIMS Analysis of Surfaces Prepared by Soft and Reactive Landing of Mass-Selected Ions (2010)

An instrument has been designed and constructed that enables in situ reactivity and time-of-flight secondary ion mass spectrometry (TOF-SIMS) analysis of surfaces prepared or modified through soft and reactive landing of mass-selected polyatomic cations and anions. The apparatus employs an electrospray ion source coupled to a high transmission electrodynamic ion funnel, two focusing collision quadrupoles, a large 19 mm diameter quadrupole mass filter, and a quadrupole bender that deflects the ion beam, thereby preventing neutral contaminants from impinging on the deposition surface. The ion soft landing apparatus is coupled to a commercial TOF-SIMS instrument permitting the introduction of surfaces into vacuum and SIMS analysis before and after ion deposition without breaking vacuum. To facilitate a comparison of the current TOF-SIMS instrument with the in situ Fourier transform ion cyclotron resonance (FTICR-SIMS) deposition apparatus constructed previously, dications of the cyclic peptide Gramicidin S (GS) and the photoactive organonometallic complex ruthenium tris-bipyridine (Ru(bpy)(3)) were soft-landed onto fluorinated self-assembled monolayer (FSAM) on gold surfaces. In both cases, similarities and differences were observed in the secondary ion mass spectra, with the TOF-SIMS results, in general, characterized by greater sensitivity, larger dynamic range, less fragmentation, and fewer in-plume reactions than the corresponding FTICR-SIMS spectra. The charge reduction kinetics of both the doubly and singly protonated GS cations on the FSAM surface were also examined as was the influence of the primary gallium ion (Ga(+)) flux on the efficiency of these processes. In addition, we demonstrate that the new instrument enables detailed studies of the reactivity of catalytically active species immobilized by soft and reactive landing toward gaseous reagents.