gebhart/patrepeval huggingface dataset:
ImmTOR nanoparticles enhance AAV transgene expression after initial and repeat dosing in a mouse model of methylmalonic acidemia (2021)

A major barrier to adeno-associated virus (AAV) gene therapy is the inability to re-dose patients due to formation of vector-induced neutralizing antibodies (Nabs). Tolerogenic nanoparticles encapsulating rapamycin (ImmTOR) provide long-term and specific suppression of adaptive immune responses, allowing for vector re-dosing. Moreover, co-administration of hepatotropic AAV vectors and ImmTOR leads to an increase of transgene expression even after the first dose. ImmTOR and AAV Anc80 encoding the methylmalonyl-coenzyme A (CoA) mutase (MMUT) combination was tested in a mouse model of methylmalonic acidemia, a disease caused by mutations in the <i>MMUT</i> gene. Repeated co-administration of Anc80 and ImmTOR was well tolerated and led to nearly complete inhibition of immunoglobulin (Ig)G antibodies to the Anc80 capsid. A more profound decrease of plasma levels of the key toxic metabolite, plasma methylmalonic acid (pMMA), and disease biomarker, fibroblast growth factor 21 (FGF21), was observed after treatment with the ImmTOR and Anc80-MMUT combination. In addition, there were higher numbers of viral genomes per cell (vg/cell) and increased transgene expression when ImmTOR was co-administered with Anc80-MMUT. These effects were dose-dependent, with the higher doses of ImmTOR providing higher vg/cell and mRNA levels, and an improved biomarker response. Combining of ImmTOR and AAV can not only block the IgG response against capsid, but it also appears to potentiate transduction and enhance therapeutic transgene expression in the mouse model.
Tuning magnetic anisotropies of Fe films on Si(111) substrate via direction variation of heating current (2013)

We adopted a novel method to tune the terrace width of Si(111) substrate by varying the direction of heating current. It was observed that the uniaxial magnetic anisotropy (UMA) of Fe films grown on the Si(111) substrate enhanced with decreasing the terrace width and superimposed on the weak six-fold magnetocrystalline anisotropy. Furthermore, on the basis of the scanning tunneling microscopy (STM) images, self-correlation function calculations confirmed that the UMA was attributed mainly from the long-range dipolar interaction between the spins on the surface. Our work opens a new avenue to manipulate the magnetic anisotropy of magnetic structures on the stepped substrate by the decoration of its atomic steps.
Adaptive duty-cycle based congestion control for home automation networks (2010)

There is a growing interest in the wireless sensor network technology in the home automation field, but as the number of sensor nodes in the home increases and as the data traffic generated by such nodes grows, the network becomes more congested. Due to resource constraints, a congestion control scheme for wireless sensor network should be designed with simplicity and operate energy efficiently. In this paper, we propose ADCC(Adaptive Duty-cycle Based Congestion Control), a lightweight congestion control scheme using duty-cycle adjustment for wireless sensor networks. This scheme uses both the resource control and traffic control approaches according to the amount of network traffic for the congestion avoidance. Our simulation results show that the ADCC improves the reliability and energy efficiency of duty-cycle based congestion control in wireless sensor networks.
Some Methods of Automatic Analysis of Television Test Signals (1971)

Investigations have been made into methods of digitally analyzing television test signals to be used in a prototype automatic monitoring equipment. To allow, wherever possible, maximum compatibility with existing manual methods, the already established insertion test signals (ITS) were employed. A preliminary survey confirmed that the component waveforms of ITS were generally amenable to digital analysis, although in some cases the approach would have to be quite different from that adopted for manual assessment. The effects of noise had to be taken into account. Suitable methods were found to be available for analyzing insertion test signals by means of an on-line digital computer to obtain the relevant transmission quality parameters.
Antigenic phenotype of myelomonocytic progenitors (CFU-GM) in chronic myeloproliferative disorders. (1986)

The antigenic phenotype of myelomonocytic progenitors [colony-forming unit granulocyte-macrophage (CFU-GM)] from 33 patients with chronic myeloproliferative disorders was investigated using four cytotoxic monoclonal antibodies. Monoclonal antibodies S3-13, S8-6, and S16-144 which recognize normal hemopoietic progenitors of different lineages reacted with almost all CFU-GM. R1B-19 monoclonal antibody identified two subpopulations of myelomonocytic progenitors (type 1 and 2 CFU-GM), as reported previously in normal subjects. In 3 of 11 patients with chronic myelogenous leukemia, in 1 of 2 patients with chronic myelomonocytic leukemia, and in 2 of 4 patients with polycythemia vera, a higher proportion of the more immature CFU-GM (type 1) was detected in bone marrow cells. The more differentiated CFU-GM (type 2) is not detectable in normal peripheral blood. By contrast, in 14 of 15 chronic myelogenous leukemia patients, in 1 of 2 chronic myelomonocytic leukemia patients and in 3 of 8 patients with idiopathic myelofibrosis, it was present in high to very high proportions. It is clear from these findings that the antigens present on normal CFU-GM are expressed in chronic myeloproliferative disorders. The proportion and distribution of type 1 and 2 CFU-GM, on the other hand, are very different from those observed in the normal subjects.
杰出的钢琴教授——马克·杜朗 (2000)

假如你很熟悉马克·杜朗(Marc Durand)的名字，这就表明你很可能是一位国际钢琴比赛的热心观众，或者说你对钢琴教学非常感兴趣。虽然这位48岁的钢琴家经常旅行、同歌唱家录音、开个人演奏会，或者参加室内音乐会的演奏，但他现在最主要的还是以钢琴教学闻名于世。事实上，他是加拿大最著名的钢琴教师之一。他的许多学生已在国际音乐舞台上尽现风采。
Differential Induction of Glioblastoma Migration and Growth by Two Forms of Pleiotrophin (2005)

Glioblastoma is the most common malignant brain tumor of adults and one of the most lethal cancers. The secreted growth factor pleiotrophin (PTN) promotes glioblastoma migration and proliferation, initiating its oncogenic activities through two cell surface receptors, the protein tyrosine phosphatase receptor zeta (PTPRZ1) and the anaplastic lymphoma kinase (ALK), respectively. Here, we report on the presence and purification of two naturally occurring forms of PTN (18 and 15 kDa) that differentially promote glioblastoma migration and proliferation. Using a panel of glioblastoma cell lines, including low passage patient-derived cultures, we demonstrate that PTN15 promotes glioblastoma proliferation in an ALK-dependent fashion, whereas immobilized PTN18 promotes haptotactic migration of glioblastoma cells in a PTPRZ1-dependent fashion. Mass spectrometric analysis indicated that PTN15 differs from PTN18 by processing of 12 C-terminal amino acids. To demonstrate clinical relevance, we show that PTN15, PTN18, and PTPRZ1 are significantly overexpressed in glioblastoma relative to normal brain at both mRNA and protein levels using microarray, Western blot, and tissue microarray analyses on human tumors. These results indicate that the PTN18-PTPRZ1 and the PTN15-ALK signaling pathways represent potentially important therapeutic targets for glioblastoma invasion and growth.
Dynamic and static aspects of solubilization of neutral arenes in ionic micellar solutions (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDynamic and static aspects of solubilization of neutral arenes in ionic micellar solutionsMats Almgren, Franz Grieser, and J. Kerry ThomasCite this: J. Am. Chem. Soc. 1979, 101, 2, 279–291Publication Date (Print):January 1, 1979Publication History Published online1 May 2002Published inissue 1 January 1979https://pubs.acs.org/doi/10.1021/ja00496a001https://doi.org/10.1021/ja00496a001research-articleACS PublicationsRequest reuse permissionsArticle Views981Altmetric-Citations581LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Proteoglycans: many forms and many functions (1992)

Proteoglycans are produced by most eukaryotic cells and are versatile components of pericellular and extracellular matrices. They belong to many different protein families. Their functions vary from the physical effects of the proteoglycan aggrecan, which binds with link protein to hyaluronan to form multimolecular aggregates in cartilage; to the intercalated membrane protein CD44 that has a proteoglycan form and is a receptor and a cell-binding site for hyaluronan; to heparan sulfate proteoglycans of the syndecan and other families that provide matrix binding sites and cell-surface receptors for growth factors such as fibroblast growth factor (FGF). One feature that recurs in proteoglycan biology is that their structure is open to extensive modulation during cellular expression. Examples of protein changes are known, but a major source of structural variation is in the glycosaminoglycan chains. The number of chains and their length can vary, as well as their pattern of sulfation. This may result in the switching of different chain types with different properties, e.g., chondroitin sulfate and heparan sulfate, and it may also result in the selective expression of sulfated chain sequences that have specific functions. The control of glycosaminoglycan structure is not well understood, but it does appear to be used to change the properties of proteoglycans to suit different biological needs. Proteoglycan forms of proteins are thus important modifiers of the organization of the pericellular and extracellular matrices and modulators of the processes that occur there.
CD4+ Th2 cells specific for mycobacterial 65-kilodalton heat shock protein protect against pristane-induced arthritis (1997)

Previous studies showed that mice with pristane-induced arthritis (PIA) and those protected from the disease by preimmunization with mycobacterial 65-kDa heat shock protein (hsp65), possess raised immune responses to hsp65. Thus, a paradox exists whereby T cells from both arthritic and hsp65-protected animals proliferate vigorously in response to the same Ag. Here we demonstrate that T cells from mice with PIA and hsp65-protected mice produce different cytokines in vitro in response to hsp65. The use of a sensitive CelELISA to measure Ag-driven lymphokine production revealed that spleen cells from hsp65-protected mice, but not those from pristane-injected or normal mice, produced the Th2-associated cytokines IL-4, IL-5, and IL-10 in response to stimulation with hsp65. By contrast, the Th1-associated cytokines IL-2 and IFN-gamma were produced by spleen cells from mice of all groups in response to hsp65. Furthermore, there was a dramatic increase in the IgG1 to IgG2a ratio of anti-hsp65 Abs from arthritic to protected mice. Thus, it appears that a Th2 response is protective against PIA. To examine this theory, a regimen of IL-12 administration which polarizes the hsp65-specific (Th2) immune response toward Th1 was identified. This regime abolished hsp65-mediated protection against PIA. Other experiments revealed that the specificity of the response to hsp65 was important, as other bacterial proteins known not to protect against PIA induced similar Th2-associated cytokines in vitro. It is considered that the protection afforded by hsp65 preimmunization is mediated by Th2-associated cytokines produced by hsp65-specific CD4+ T cells.
Detection of human serum tumor necrosis factor-alpha in healthy donors, using a highly sensitive immuno-PCR assay. (1999)

This method may be useful for analyzing the significance of TNFalpha concentration in various diseases.
Selective Depositions on Polyelectrolyte Multilayers:  Self-Assembled Monolayers of m-dPEG Acid as Molecular Template (2004)

This paper describes the fabrication of self-assembled monolayer (SAM) patterns of m-d-poly(ethylene glycol) (m-dPEG) acid molecules onto polyelectrolyte multilayers (PEMs). The patterned SAMs on PEMs were created by ionic interactions using microcontact printing (microCP) technique. The created m-dPEG acid monolayer patterns on PEMs act as resistive templates, and thus further depositions of consecutive poly(anion)/poly(cation) pairs of charged particles result in the formation of three-dimensional (3-D) patterned PEM films or selective particle depositions atop the original multilayer thin films. In this study, we illustrate nonlithographic methods of patterning and controlling 3-D PEM architectures and selective particle depositions. We investigated the effect of variables--the choice of solvent, concentration, pH, substrate pretreatment, and stamp contact times--on microcontact printing of m-dPEG acid molecules onto PEM films to determine the optimal conditions for these parameters to achieve efficient transfer of m-dPEG acid patterns onto PEMs. Among the variables, the pH of the m-dPEG acid ink solution played the most important role in the transfer efficiency of the patterns onto the multilayer films. The patterned films were characterized by optical microscopy and atomic force microscopy (AFM).
Growth of Isolated Carbon Nanotubes with Lithographically Defined Diameter and Location (2003)

The synthesis of individual carbon nanotubes (CNTs) with controlled diameters at lithographically predefined locations has been achieved. Employing conventional catalyst-mediated chemical vapor deposition (CVD), isolated CNTs were grown out of nanoholes in silicon dioxide created by optical lithography. This allows the precise placement of individual CNTs on substrates. Furthermore, the diameter of each CNT adjusts to the hole size, which makes it possible to control this important property separately for individual CNTs. This approach is a step toward the parallel manufacture of nanotube-based devices in a scalable batch process. In particular, the integration of CNTs into conventional silicon-technology with potential applications as interconnects, transistors, and memory cells becomes possible.
Molecular cloning and functional expression of a brain-specific somatostatin receptor. (1992)

The PCR and conventional library screening were used to clone the brain-specific somatostatin receptor rSSTR-4 from a rat genomic library. The deduced amino acid sequence encodes a protein of 384 amino acids and displays structural and sequence homologies with members of the G protein-receptor superfamily. The amino acid sequence of rSSTR-4 is 60% and 48% identical to that of somatostatin receptors SSTR-1 and SSTR-2, respectively, two recently cloned subtypes. Competition curve analysis of the binding properties of the receptor transiently expressed in COS-1 cells revealed a higher apparent affinity for somatostatin 14 than for somatostatin 28. In contrast, the somatostatin analogs SMS 201-995, IM 4-28, and MK-678 failed to displace specific binding in transfected cells. These characteristics resemble the pharmacological binding properties of the previously described brain-specific somatostatin-receptor subtype. Examination of the tissue distribution of mRNA for rSSTR-4 revealed expression limited to various brain regions with highest levels in the cortex and hippocampus. Thus, based on the pharmacology and tissue localization of this receptor, we conclude that rSSTR-4 represents a brain-specific somatostatin receptor.
Identification of a Bipartite Jasmonate-Responsive Promoter Element in the Catharanthus roseus ORCA3 Transcription Factor Gene That Interacts Specifically with AT-Hook DNA-Binding Proteins (2007)

Jasmonates are plant signaling molecules that play key roles in defense against certain pathogens and insects, among others, by controlling the biosynthesis of protective secondary metabolites. In Catharanthus roseus, the APETALA2-domain transcription factor ORCA3 is involved in the jasmonate-responsive activation of terpenoid indole alkaloid biosynthetic genes. ORCA3 gene expression is itself induced by jasmonate. By loss- and gain-of-function experiments, we located a 74-bp region within the ORCA3 promoter, which contains an autonomous jasmonate-responsive element (JRE). The ORCA3 JRE is composed of two important sequences: a quantitative sequence responsible for a high level of expression and a qualitative sequence that appears to act as an on/off switch in response to methyl jasmonate. We isolated 12 different DNA-binding proteins having one of four different types of DNA-binding domains, using the ORCA3 JRE as bait in a yeast (Saccharomyces cerevisiae) one-hybrid transcription factor screening. The binding of one class of proteins bearing a single AT-hook DNA-binding motif was affected by mutations in the quantitative sequence within the JRE. Two of the AT-hook proteins tested had a weak activating effect on JRE-mediated reporter gene expression, suggesting that AT-hook family members may be involved in determining the level of expression of ORCA3 in response to jasmonate.
The effect of spin on the flight of a baseball (2008)

Measurements are presented of the Magnus force on a spinning baseball. The experiment utilizes a pitching machine to project the baseball horizontally, a high-speed motion analysis system to determine the initial velocity and angular velocity and to track the trajectory over 5m of flight, and a ruler to measure the total distance traversed. Speeds in the range v=50–110mph and spin rates ω (topspin or backspin) in the range 1500–4500rpm were utilized, corresponding to Reynolds numbers of Re=(1.1–2.4)×105 and spin factors S≡Rω∕v in the range 0.090–0.595. Least-squares fits were used to extract the initial parameters of the trajectory and to determine the lift coefficients. Comparison is made with previous measurements and parametrizations, and implications for the effect of spin on the flight of a baseball are discussed. The lift coefficient CL is found not to depend strongly on v at fixed values of S.
Electrokinetically Driven Microfluidic Chips with Surface-Modified Chambers for Heterogeneous Immunoassays (2001)

This article presents the first example of a microfluidic chip for heterogeneous bioassays using a locally immobilized biospecific layer and operated electrokinetically. The reaction chamber has picoliter dimensions and is integrated into a network of microchannels etched in glass. The high affinity of protein A (PA) for rabbit immunoglobulin G (rIgG) was exploited for chip testing, with PA being immobilized on microchannel walls and fluorescently labeled (Cy5) rIgG serving as sample. It was possible to operate the chip in an immunoaffinity chromatographic manner, using electrokinetically pumped solutions. Concentration of antibody from dilute solution onto the solid phase was demonstrated, with signal gains of approximately 30 possible. A dose-response curve for Cy5-rIgG was obtained for concentrations down to 50 nM, for an incubation time of 200 s. The flexibility of chip layout was demonstrated for competitive immunoassay of rIgG, using both a combined sample/tracer incubation and sequential addition of these solutions. With assay times generally below 5 min for this unoptimized device, the microfluidic approach described shows great potential for many high-throughput screening applications.
Obstructive sleep apnoea: diagnosis and management (1997)

Snoring and the associated syndrome of sleep apnoea can have severe effects on the lives of sufferers and their families. This article discusses why obstructive sleep apnoea is potentially fatal, and how it may be identified and managed.
A THEORY OF THE PATHOGENESIS OF ORDINARY HUMAN BALDNESS (1950)

BALDNESS in men is such a common occurrence that it has almost come to be accepted as a normal phenomenon. This view has been encouraged by the attributing of the condition either to irrevocable heredity or to the ubiquitous condition of seborrhea. Understandably, however, much thought and investigation have been expended in an attempt to comprehend and reverse this blow to masculine pride. Work on the subject has been carried out almost exclusively by dermatologists. A review of the most authoritative present day dermatologic textbooks indicates, however, that the problem is still far from being solved. We, as psychiatrists, were initially drawn to this problem by observations on facial expression. These observations, together with certain significant contributions to the literature which were apparently hitherto overlooked, led to the formulation of what is offered as a comprehensive explanation of the pathogenesis of ordinary human baldness. CRITICAL REVIEW OF THE LITERATURE The literature dealing with the problem of ordinary
Novel Biodegradable Shape‐Memory Elastomers with Drug‐Releasing Capabilities (2011)

Novel polydiolcitrate elastomers with thermally induced shape-memory properties are synthesized. These thermoresponsive polymers can be prepared as films or porous 3D sponges. Above their transition temperature, these materials display an elastomeric behavior, but below their transition temperature they have a glassy state. This thermally induced transition allows for the acquisition of their dual shape. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
An o-quinone form of estrogen produces free radicals in human breast cancer cells: Correlation with DNA damage (1994)

The o-quinone forms of 2,3- and 3,4-catechol estrogens have been implicated in the carcinogenicity of these hormones. The concomitant production of reactive oxygen species during reduction of the o-quinone estrogens has been inferred to play a mechanistic role in their mutagenic potential. Conclusive evidence documenting the production of hydrogen peroxide, the hydroxyl radical, and the estrone 3,4-semiquinone in estrone 3,4-quinone (3,4-EQ)-treated human breast cancer subcellular fractions was demonstrated in the absence of exogenously added catalysts. Subcellular fractions of MCF-7 cells treated with 3,4-EQ and NADPH, including nuclei, mitochondria, and microsomes, were shown to support significant amounts of hydrogen peroxide production. Hydrogen peroxide production in 3,4-EQ-treated cellular fractions and the chromosomal DNA damage induced in 3,4-EQ-treated MCF-7 cells were abolished by the addition of catalase. A significant and potentially physiologically relevant spontaneous reduction of 3,4-EQ by NADPH resulting in hydrogen peroxide production was demonstrated. The results unequivocally demonstrate that free radicals are produced during the metabolism of estrone 3,4-quinone in human cells.