gebhart/patrepeval huggingface dataset:
Heritable major histocompatibility complex class II-associated differences in production of tumor necrosis factor alpha: relevance to genetic predisposition to systemic lupus erythematosus. (1990)

We report on the production of tumor necrosis factor (TNF)-alpha and TNF-beta by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- and DQw1-positive donors frequently exhibit low production of TNF-alpha, whereas DR3- and DR4-positive subjects show high levels of TNF-alpha production. No correlation between TNF-alpha levels and HLA-A, -B, and -C genotype was found. The relevance of this quantitative polymorphism to the genetic predisposition to lupus nephritis in systemic lupus erythematosus (SLE) patients was investigated. DR2, DQw1-positive SLE patients show low levels of TNF-alpha inducibility; this genotype is also associated with an increased incidence of lupus nephritis. DR3-positive SLE patients, on the other hand, are not predisposed to nephritis, and these patients have high TNF-alpha production. DR4 haplotype is associated with high TNF-alpha inducibility and is negatively correlated with lupus nephritis. These data may help explain the strong association between HLA-DR2, DQw1 in SLE patients and their susceptibility to nephritis.
Particulate Bioglass® reduces the viability of bacterial biofilms formed on its surface in an in vitro model (2002)

45S5 Bioglass is a bioactive implant material which, in its particulate form, is used in the repair of periodontal defects. The surface reactions undergone by this material in an aqueous environment may exert an antibacterial effect that would be beneficial to periodontal surgical treatment. Biofilms of Streptococcus sanguis, an early plaque former, and mixed species biofilms from a salivary inoculum grown under conditions similar to those associated with periodontal implants, were grown on particulate Bioglass in a constant depth film fermenter (CDFF). Control biofilms were grown on inert glass particulates. At sample times of 3, 24 and 48 hours the viability of biofilms of S. sanguis grown on Bioglass was significantly lower than for those grown on inert glass. In the experiments with subgingivally-modelled mixed species biofilms, the total anaerobic counts were significantly lower on Bioglass after 24 and 48 hours, but not 96 or 168 hours, compared to inert glass. Thus, particulate Bioglass has the potential to reduce bacterial colonisation of its surface in vivo, a feature relevant to post-surgical periodontal wound healing.
Nanoparticle Targeting of Anticancer Drug Improves Therapeutic Response in Animal Model of Human Epithelial Cancer (2005)

Prior studies suggested that nanoparticle drug delivery might improve the therapeutic response to anticancer drugs and allow the simultaneous monitoring of drug uptake by tumors. We employed modified PAMAM dendritic polymers <5 nm in diameter as carriers. Acetylated dendrimers were conjugated to folic acid as a targeting agent and then coupled to either methotrexate or tritium and either fluorescein or 6-carboxytetramethylrhodamine. These conjugates were injected i.v. into immunodeficient mice bearing human KB tumors that overexpress the folic acid receptor. In contrast to nontargeted polymer, folate-conjugated nanoparticles concentrated in the tumor and liver tissue over 4 days after administration. The tumor tissue localization of the folate-targeted polymer could be attenuated by prior i.v. injection of free folic acid. Confocal microscopy confirmed the internalization of the drug conjugates into the tumor cells. Targeting methotrexate increased its antitumor activity and markedly decreased its toxicity, allowing therapeutic responses not possible with a free drug.
Digital radiofrequency echocardiography in the detection of myocardial contrast following intravenous administration of Albunex(R) (1993)

Conventional contrast echo techniques have proved inadequate for the detection of myocardial perfusion using intravenously injected echo contrast agents because of the limitations and relative insensitivity of standard echocardiographic equipment. In order to avoid these problems, we have obtained pure digital radiofrequency ultrasound data from the left ventricle and myocardium during i.v. contrast myocardial perfusion echo studies. In 30 patients, following coronary arteriography, i.v. injections of the echo contrast agent Albunex (sonicated human serum albumin) in two doses of 0.08 and 0.22 ml.kg-1 were administered during digital radiofrequency echocardiography sampling of data from the myocardium and left ventricular cavity. Analysis of mean integrated backscatter (MIB, a measure of the total ultrasound energy) was performed before, during and after Albunex injection. The data were also analysed for a shift in frequency spectrum which could be caused by resonance of the Albunex contrast microspheres in the heart, a phenomenon which has been previously demonstrated in vitro. Digital radiofrequency ultrasound data were successfully obtained and analysed in 23 patients. In 31 segments where reasonable resting perfusion was expected, there was a significant increase in MIB from 0.644 to 1.245, P < 0.001 and time intensity curves could be constructed showing wash-in and wash-out of contrast from the myocardium. In 10 segments supplied by significantly diseased vessels, MIB intensity increased from 1.044 to 1.874, P = 0.054. In myocardial segments supplied by non-diseased vessels, microsphere resonance also caused a drop in mean frequency of 140 KHz, P < 0.001, and permitted similar temporal analysis of myocardial perfusion.(ABSTRACT TRUNCATED AT 250 WORDS)
Building the isotropic acoustic potential well with strong constraint boundary to improve the stability of ultrasonic transportation (2013)

A quantitative analysis of the acoustic potential well has been proposed for the purpose of realizing stability improvement of ultrasonic transportation. It was found that the boundary Rp and elastic constant kl(l,θ) of the acoustic potential well, acoustic radiation force offset ratio βfl, and elastic constant offset ratio βkl are the critical parameters that define the trapping ability. They were made clear both their intrinsic significance. The stability of the ultrasonic transportation using three transducers is theoretically studied. Long range ultrasonic transportation of silica beads with better stability is realized by optimizing the acoustic parameters to get the well-defined acoustic potential wells. No slip-off the equilibrium position has been observed, which proved its strong ability of trapping and transportation. Because of its simplicity, flexibility, and non-destructivity, the ultrasonic transportation offered a competitive micro-manipulation technology and will provide a promising tool for life science, chemistry and micro-assembly.
Simplified Method for the Preparation of Aromatic Sulfuric Acid Esters (1941)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSimplified Method for the Preparation of Aromatic Sulfuric Acid EstersJ. Feigenbaum and C. A. NeubergCite this: J. Am. Chem. Soc. 1941, 63, 12, 3529–3530Publication Date (Print):December 1, 1941Publication History Published online1 May 2002Published inissue 1 December 1941https://doi.org/10.1021/ja01857a508RIGHTS & PERMISSIONSArticle Views1157Altmetric-Citations90LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (247 KB) Get e-Alertsclose Get e-Alerts
The fabrication of metal–oxide–semiconductor transistors using cerium dioxide as a gate oxide material (1991)

Cerium dioxide was employed as a gate insulator for an enhancement-type n-channel metal–oxide–semiconductor (MOS) transistor. Cerium was evaporated in a tungsten boat and immediately oxidized for oxide uniformity. The use of CeO2 as a gate oxide in MOS transistor yielded a low positive threshold voltage with negligible interface charge effects. This resulted in the transistor performing as an enhancement type device.
Painful Sensory Neuropathy (2003)

A 67-year-old woman who had been in excellent health noticed the onset of burning pain in the left great toe two years before evaluation. The pain subsequently extended to involve both feet, from the toes to the heels, and was associated with numbness, tingling, and burning. The discomfort has become severe, is present throughout the day, and disrupts sleep. A physical examination reveals normal muscle strength, muscle-stretch reflexes, proprioception, and vibratory sensation; only pinprick sensation in the toes and feet is diminished. How should this patient be evaluated and treated?
Towards emotional speech synthesis: a rule based approach. (2004)

This note describes a framework used to simulate three basic emotional styles by means of prosodic transplantation techniques applied to the output of a corpus based speech synthesis system. The target pitch profiles together with duration and energy constraints have been obtained applying simple rules inferred from the analysis of a small corpus, recorded in three emotional styles. Results of perceptual tests show that styles are well recognized even if the acoustical quality, in some cases, degrades.
Escherichia coli and Salmonella :cellular and molecular biology (1996)

Preface The Enteric Bacterial Cell and the Age of Bacteria Variations on a Theme by Escherichia Part I: Molecular Architecture and Assembly of Cell Parts (11 chapters) Part II: Metabolism and General Physiology (58 chapters) Part III: Utilization of Energy for Cell Activities (7 chapters) Part IV: Regulation of Gene Expression (19 chapters) Part V: Growth of Cells and Cultures (12 chapters) Part VI: Genome, Genetics and Evolution (40 chapters) Part VII: Molecular Pathogenesis (7 chapters)
Broadband wireless access and future communication networks (2001)

This paper presents a vision for wireless communication systems beyond the third generation, which comprises a combination of several optimized access systems on a common IP-based medium-access and core network platform. These different access systems will interwork via horizontal and vertical handover, service negotiation, and global roaming. The different access systems are allocated to different cell layers in the sense of hierarchical cells with respect to cell size, coverage, and mobility to provide globally optimized seamless services to all users. This vision requires extensive international research and standardization activities to solve many technical challenges. Key issues are the global interworking of different access systems on a common platform, the implementation of multimode and multiband terminals and base stations by software-defined radio concepts as well as advanced antenna concepts.
Association between complement factor H gene polymorphisms and neovascular age-related macular degeneration in Koreans (2008)

PURPOSE. This study was undertaken to investigate the association between the complement factor H (CFH) gene and exudative age-related macular degeneration (AMD) in Korean patients. METHODS. Genomic DNA was isolated from the peripheral leukocytes of patients with exudative AMD (n 114) and control subjects (n 187). The sole criterion for exudative AMD was the presence of choroidal neovascularization. Four single-nucleotide polymorphisms (SNPs: 275CT, I62V, Y402H, and IVS15) located in promoter, exon 2, exon 9, and intron 15 of the CFH gene were genotyped by PCR-based direct sequencing. RESULTS. The frequency of the C allele of Y402H (AMD, 10.5%; control, 6.5%) was found to be lower in Koreans than in Caucasians. In the present study, the difference between the frequencies of Y402H in cases and control subjects did not reach statistical significance (P 0.071). However, the frequencies of the major alleles of three SNPs (275CT, I62V, and IVS15) were significantly different in patients and control subjects, and these SNPs were found to be separately associated with an elevated risk of exudative AMD. Seven haplotypes were identified in Koreans. Haplotype analysis showed that two haplotypes (TGTG, CGTG) conferred significantly higher risks of exudative AMD (P 0.013, 0.035), and one haplotype (CATA) was significantly protective (P 0.001). CONCLUSIONS. In Korean subjects, CFH polymorphism appears to be a considerable hereditary contributor to exudative AMD. Y402H polymorphism which has been suggested to be a major risk factor of AMD in Caucasians was found to be only marginally associated with exudative AMD with low frequency, whereas three adjacent SNPs in the CFH gene were significantly associated with AMD in Koreans. (Invest Ophthalmol Vis Sci. 2008;49:2071‐2076) DOI:10.1167/iovs.07-1195 A ge-related macular degeneration (AMD) is the primary cause of severe vision loss in the elderly. It commonly occurs in patients older than 50 years and usually affects both eyes. 1 AMD is a genetically complex disorder of the photoreceptor-retinal pigment epithelium (RPE)-Bruch’s membranechoriocapillaris complex. Early signs are characterized by the presence of soft drusen, areas of increased pigment or hyperpigmentation (in the outer retina or choroid), and/or areas of depigmentation or hypopigmentation of the RPE. Advanced disease manifests either as geographic atrophy or choroidal neovascularization. 2 Several risk factors, including aging, cigarette smoking, and arterial hypertension, have been proposed, but only a fraction
Poly-FISH: a technique of repeated hybridizations that improves cytogenetic analysis of fetal cells in maternal blood (1998)

Prenatal diagnosis of fetal chromosomal abnormalities using interphase fetal nucleated erythrocytes (FNRBCs) separated from maternal peripheral blood can be technically challenging due to the limited number of FNRBCs available for analysis, the limited number of probes that can be used simultaneously, and low FISH efficiency on the formaldehyde-fixed and immunohistochemically stained interphase FNRBCs. We developed a technique of sequential FISH analysis that involves removal of the previous hybridized probe under denaturing conditions, and rehybridization with different probes to improve FISH efficiency. This technique facilitates the analysis of multiple chromosome-specific probes on the same nuclei. Results from our experiments show that FISH can be performed at least nine times on the same interphase nucleus and at least three different probes can be used simultaneously. Thus, theoretically, at least 24 different chromosomes can be analysed on a single interphase fetal cell isolated from maternal blood. We have termed this technique 'Poly-FISH', and have successfully diagnosed trisomy 21, triploidy, and other chromosome abnormalities in FNRBCs using this technique.
Low Power, Multi-Gigabit DRAM Cell Design Issues Using SOI Technologies (1999)

Abstract: Silicon On Insulator (SOI) can leverage a lot of new advantages for circuit designers compared to conventional bulk technology. In particular, the improved S-factor and reduced junction capacitance make it very appealing for next generation low power, high performance DRAM systems. However, the benefits of the SOI technology do not come entirely for free. In this project, we characterized the key parameters of SOI devices in regards to DRAM applications, and simulated various control schemes used in DRAMs for different SOI technologies. As expected our results show a large improvement in bit line capacitance for the SOI devices compared to the bulk devices. A comparison of the different SOI technologies shows a significant overall advantage of using fully depleted SOI devices compared to partially depleted SOI in terms of both power and performance. 1.
Structure-based engineering of a monoclonal antibody for improved solubility (2010)

Protein aggregation is of great concern to pharmaceutical formulations and has been implicated in several diseases. We engineered an anti-IL-13 monoclonal antibody CNTO607 for improved solubility. Three structure-based engineering approaches were employed in this study: (i) modifying the isoelectric point (pI), (ii) decreasing the overall surface hydrophobicity and (iii) re-introducing an N-linked carbohydrate moiety within a complementarity-determining region (CDR) sequence. A mutant was identified with a modified pI that had a 2-fold improvement in solubility while retaining the binding affinity to IL-13. Several mutants with decreased overall surface hydrophobicity also showed moderately improved solubility while maintaining a similar antigen affinity. Structural studies combined with mutagenesis data identified an aggregation 'hot spot' in heavy-chain CDR3 (H-CDR3) that contains three residues ((99)FHW(100a)). The same residues, however, were found to be essential for high affinity binding to IL-13. On the basis of the spatial proximity and germline sequence, we reintroduced the consensus N-glycosylation site in H-CDR2 which was found in the original antibody, anticipating that the carbohydrate moiety would shield the aggregation 'hot spot' in H-CDR3 while not interfering with antigen binding. Peptide mapping and mass spectrometric analysis revealed that the N-glycosylation site was generally occupied. This variant showed greatly improved solubility and bound to IL-13 with affinity similar to CNTO607 without the N-linked carbohydrate. All three engineering approaches led to improved solubility and adding an N-linked carbohydrate to the CDR was the most effective route for enhancing the solubility of CNTO607.
Overview and Discovery of IgNARs and Generation of VNARs (2012)

Immunoglobulin new antigen receptors (IgNARs) from sharks are a distinct class of immune receptors, consisting of homodimers with no associated light chains. Antigen binding is encapsulated within single VNAR immunoglobulin domains of 13-14 kDa in size. This small size and single domain format means that they exhibit considerable stability and are readily produced in heterologous protein expression systems. In this chapter, I describe the history and discovery of IgNARs, the development of VNAR biotechnology, and highlight important factors in VNAR protein production.
Monofunctional Substrates of Polynucleotide Phosphorylase (1971)

Polynucleotide phosphorylase from Escherichia coli cells catalyzes the transfer of a single nucleotidyl residue from each of the 2′(3′)‐ O ‐isovalerylesters of ADP, GDP, CDP and UDP to the initiator oligonucleotide, A‐A‐A. The products of these reactions are identified as the 2′(3′)‐ O ‐isovaleryl esters of the corresponding tetranucleotides A‐A‐A‐A, A‐A‐A‐G, A‐A‐A‐C and A‐A‐A‐U. The 2′(3′)‐ O ‐isovaleryl residue can be removed from the product by treatment with aqueous methanolic ammonia under conditions that do not significantly damage the oligonucleotide chains. The monoaddition of 2′(3′)‐ O ‐acyl esters of nucleoside diphosphates to an oligonucleotide acceptor is proposed as a method for the stepwise synthesis of oligoribonucleotides of defined sequence.
FT-Raman Spectroscopy of Wood: Identifying Contributions of Lignin and Carbohydrate Polymers in the Spectrum of Black Spruce (Picea Mariana) (1997)

Good-quality Raman spectra of most wood species can now be obtained by using near-infrared Fourier transform Raman spectroscopy. To make effective use of such spectroscopic information, one needs to interpret the data in terms of contributions from various wood components and, for each component polymer, in terms of vibrational modes of its substructural units/groups. In the present work, Raman spectral features of black spruce (Picea mariana) wood were associated with lignin and/or carbohydrate polymers. Lignin's spectral contributions were recognized in several ways. In addition to spectra of milled-wood and enzyme lignins, a spectrum of native lignin was obtained by subtracting the spectrum of acid chlorite delignified black spruce from the spectrum of an untreated wood sample. A comparison of lignin spectra indicated that the Raman features of the three lignins are very similar. Raman contributions of carbohydrate polymers, namely, those of cellulose and hemicellulose, were identified by using authentic and/or isolated samples and, in the case of cellulose, by using previously published spectra. Such an analysis showed that the hemicellulose present in black spruce did not give rise to any new, unique features that were not already present due to cellulose. Therefore, it was concluded that the hemicellulose contribution is broad and is hidden under the Raman contribution of cellulose. Also, peak positions of lignin contributions did not overlap with those of cellulose, and there were spectral regions where either lignin or cellulose contributed.
Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria (1979)

Summary: On the basis of a comparative study of 178 strains of cyanobacteria, representative of this group of prokaryotes, revised definitions of many genera are proposed. Revisions are designed to permit the generic identification of cultures, often difficult through use of the field-based system of phycological classification. The differential characters proposed are both constant and readily determinable in cultured material. The 22 genera recognized are placed in five sections, each distinguished by a particular pattern of structure and development. Generic descriptions are accompanied by strain histories, brief accounts of strain properties, and illustrations; one or more reference strains are proposed for each genus. The collection on which this analysis was based has been deposited in the American Type Culture Collection, where strains will be listed under the generic designations proposed here.