gebhart/patrepeval huggingface dataset:
Effect of direct injection of cyclosporine on the arterial vessels of the isolated hind limb in the dog. (1991)

Cyclosporine's toxic side effects are widely recognized as a cause of major morbidity. Peripheral vasoconstriction has been suggested as the pathophysiological mechanism for hypertension and renal failure caused by cyclosporine. To study vascular effects on peripheral arterial circulation, cyclosporine was injected into isolated hind limbs perfused at constant flow in the dog. Pure powder cyclosporine was dissolved in a 10% fat emulsion and infused directly into the arterial inflow of the perfused hind limb (n = 7) at a concentration of 5 mg/kg body weight. Reactivity of the vascular bed was first shown by an average decrease of 72 +/- 4 mmHg (95% confidence interval 63 to 81, P less than 0.01) in hind limb perfusion pressure after infusion of 5 mg nitroglycerin. Infusion of fat emulsion did not cause any significant changes. The infusion of cyclosporine caused an average increase of 29 +/- 5 mmHg (95% confidence interval 17 to 41, P less than 0.01) in hind limb perfusion pressure after 4 mins' infusion. After cyclosporine infusion, the vascular bed still responded to nitroglycerin by an average decrease of 56 +/- 5 mmHg (95% confidence interval 42 to 69, P less than 0.01) in perfusion pressure. Blockade of alpha-receptors with phentolamine in five dogs and ipsilateral lumbar sympathectomy in four prevented the increase in perfusion pressure following cyclosporine injection. In conclusion, cyclosporine injected at high doses causes a small vasoconstriction of the peripheral arterial circulation in the hind limb through stimulation of alpha-adrenergic receptors mediated by the sympathetic nervous system. Since the response is completely abolished by sympathectomy, it is probably caused by reflex activation. A direct effect of cyclosporine on the arterial vessel walls of the limb can therefore be excluded.
The nature of the binding site for aromatic compounds in glycogen phosphorylase b (1975)

The inhibition of rabbit muscle glycogen phosphorylase b (1,4-alpha-D-glucan--orthophosphate alpha-glucosyltransferase, EC 2.4.1.1) by aromatic compounds was examined with 15 compounds. The relative effectiveness of the inhibitors correlated well with increasing substituent constant, pi, indicating the hydrophobic nature of the binding site. The inhibition was not affected by the ionic-strength variation of the assay mixtures. The results predict that the course of chemical modification of this enzyme and the properties of the derivatives depend on the nature of the reagent and on the incorporated groups. Many of the dissimilar and sometimes contradictory results reported for chemical-modification studies and for chemically modified phosphorylase b are explained by the findings presented in the paper.
Human transferrin. Expression and iron modulation of chimeric genes in transgenic mice. (1990)

Transferrin (TF) is a plasma protein that transports and is regulated by iron. The aim of this study was to characterize human TF gene sequences that respond in vivo to cellular signals affecting expression in various tissues and to iron administration. Chimeric genes were constructed containing 152, 622, and 1152 base pairs (bp) of the human TF5'-flanking region with the coding region of a reporter gene, CAT (chloramphenicol acetyltransferase), and introduced into the germ line of mice. Transgenes containing TF 5'-flanking sequences to -152 bp were expressed poorly in all tissues examined. In contrast, transgenes containing TF sequences to -622 or -1152 bp were expressed at high levels in brain and liver, greater than or equal to 1000-fold higher than tissues such as heart and testes. Liver and brain are major sites of endogenous TF mRNA synthesis, but liver mRNA levels are 10-fold higher than brain. A significant diminution of CAT enzymatic activity in liver accompanied iron administration in both TF(0.67) and TF(1.2)CAT transgenic mice, mimicking the decrease of transferrin in humans following iron overload. Levels of endogenous plasma transferrin also decreased in iron-treated transgenic mice. Transgenic mouse lines carrying human TF chimeric genes will be useful models for analyzing the regulation of human transferrin by iron and for determining the molecular basis of transferrin regulation throughout mammalian development into the aging process.
Balloon‐assisted tracking of a guide catheter through difficult radial anatomy: A technical report (2012)

A small caliber or significant tortuosity of radial artery may create difficulty in negotiating a 6F or 7F guide catheter while performing intervention through transradial approach. We describe a technique that helps tracking a guide catheter in such demanding situation for successful completion of procedure without increasing the chance of perforation and dissection.
MR ENDPLATE CHANGES ARE ASSOCIATED WITH INCREASED DISC INFLAMMATORY MEDIATOR PRODUCTION (2003)

Recently there has been considerable interest in the role of inflammatory mediator production by herniated degenerate discs. Modic has described MR endplate changes which have an inflammatory appearance and have been linked with discogenic back pain. To date there has been no biomechanical investigation of discs with associated Modic changes. The aim of this study is to determine if degenerate discs with associated Modic changes have higher levels of pro-inflammatory mediator production than those without Modic changes. Intervertebral disc tissue was obtained from 52 patients undergoing spinal surgery for sciatica [40] and discogram proven discogenic low back pain [12]. The tissue was cultured and the medium analysed for interleukin-6, interleukin-8 and prostaglandin E2 using an enzyme linked immunoabsorbetn assay method. Preoperative MR images of the patients were examined by a double blinded radiologist to determine the Modic status of the cultured disc level. Forty percent of patients undergoing surgery for discogenic low back pain had a Modic 1 change compared to only 12.5% of patients undergoing surgery for sciatica [p .05, PGE2: NEG v M1 p Modic changes have been associated with positive provocative discography by a number of authors. Pain generation requires the presence of nerves and hyperalgsia inducing mediators. Both IL-8 and PGE2 are known to induce hyperalgesia. The fact that Modic changes are associated with high levels of production of these mediators supports their role as an objective marker of discogenic low back pain.
A Porous and Interpenetrated Metal–Organic Framework Comprising Tetranuclear IronIII–Oxo Clusters and Tripodal Organic Carboxylates and Its Implications for (3,8)-Coordinated Networks (2007)

A porous metal–organic framework (MOF), Fe4(μ3-O)2(BTB)8/3(DMF)2(H2O)2·(DMF)10(H2O)2 (1) (BTB = benzene-1,3,5-tribenzoate, DMF = N,N-dimethylformamide) has two interpenetrating 3D frameworks composed of fused cages and shows a stronger affinity for CO2 than most other known highly porous MOFs. The unique tetranuclear iron–oxo clusters act as 8-connectors, which are linked by 3-connecting BTBs, to give a partially augmented the net (using the symbolism of O’Keeffe and co-workers). The underlying implications for (3,8)-coordinated networks are also discussed in relation to the framework structure of 1.
Continuous-Flow Chemical Processing on a Microchip by Combining Microunit Operations and a Multiphase Flow Network (2002)

A new design and construction methodology for integration of complicated chemical processing on a microchip was proposed. This methodology, continuous-flow chemical processing (CFCP), is based on a combination of microunit operations (MUOs) and a multiphase flow network. Chemical operations in microchannels, such as mixing, reaction, and extraction, were classified into several MUOs. The complete procedure for Co(II) wet analysis, including a chelating reaction, solvent extraction, and purification was decomposed into MUOs and reconstructed as CFCP on a microchip. Chemical reaction and molecular transport were realized in and between continuous liquid flows in a multiphase flow network, such as aqueous/aqueous, aqueous/organic, and aqueous/organic/aqueous flows. When the determination of Co(II) in an admixture of Cu(II) was carried out using this methodology, the determination limit (2sigma) was obtained as 18 nM, and the absolute amount of Co chelates detected was 0.13 zmol, that is, 78 chelates. The sample analysis time was faster than that of a conventional processing system. Moreover, troublesome operations such as phase separation and acid and alkali washing, all necessary for the conventional system, were simplified. The CFCP methodology proposed here can be applied to various on-chip applications.
Compensatory Roles of Foxa1 and Foxa2 during Lung Morphogenesis (2005)

Foxa1 and Foxa2 are closely related family members of the Foxa group of transcription factors that are coexpressed in subsets of respiratory epithelial cells throughout lung morphogenesis. Shared patterns of expression, conservation of DNA binding, and transcriptional activation domains indicate that they may serve complementary functions in the regulation of gene expression during lung morphogenesis. Whereas branching morphogenesis of the fetal lung occurs normally in the Foxa2Delta/Delta and Foxa1-/- mice, deletion of both Foxa1 and Foxa2 (in Foxa2Delta/Delta, Foxa1-/- mice) inhibited cell proliferation, epithelial cell differentiation, and branching. Dilation of terminal lung tubules and decreased branching were observed as early as embryonic day 12.5. Foxa1 and Foxa2 regulated Shh (sonic hedgehog) and Shh-dependent genes in the respiratory epithelial cells that influenced the expression of genes in the pulmonary mesenchyme that are required for branching morphogenesis. Epithelial cell differentiation, as indicated by lack of expression of surfactant protein B, surfactant protein C, the Clara cell secretory protein, and Foxj1, was inhibited. Foxa family members regulate signaling and transcriptional programs required for morphogenesis and cell differentiation during formation of the lung.
Are prenyl groups on proteins sticky fingers or greasy handles? (2003)

This Commentary discusses the work of Dietrich et al. in this issue of the Biochemical Journal, which sheds new light on the biological roles of protein-bound prenyl groups by providing evidence that the α-subunit of the heterotrimeric G-protein transducin has a binding site for the geranylgeranyl group of the γ-subunit.
Genotypic Influences on Severity of Exudative Age-Related Macular Degeneration (2010)

This study demonstrates an association between the at-risk allele of the ARMS2/LOC387715 locus and classic CNV, fibrovascular lesions, and poor VA. Individuals double homozygous for both at-risk alleles had a higher risk of being affected with a severe form of AMD at an earlier age.
Experiences with a high-speed network adaptor (1994)

This paper describes our experiences, from a software perspective, with the OSIRIS network adaptor. It first identifies the problems we encountered while programming OSIRIS and optimizing network performance, and outlines how we either addressed them in the software, or had to modify the hardware. It then describes the opportunities provided by OSIRIS that we were able to exploit in the host operating system (OS); opportunities that suggested techniques for making the OS more effective in delivering network data to application programs. The most novel of these techniques, called application device channels , gives application programs running in user space direct access to the adaptor. The paper concludes with the lessons drawn from this work, which we believe will benefit the designers of future network adaptors.
Disintegration of dextran sulfate tablet products: Effect of physicochemical properties (1992)

Disintegration characteristics of three lots of enteric-coated dextran sulfate tablets (250 or 300 mg dosage formulations) from two manufacturers (A and B) were determined in 0.1M phosphate buffer at various pH levels between 5.0 and 7.5. Differences in disintegration times within- and between-lots were observed and appear to relate to differences in the nature and thickness of their enteric coatings. The composition of tablets and coatings was examined by Fourier-transform infrared spectroscopy. Lactose and a form of cellulose were the predominant excipients in the A and B formulations, respectively. The yellow coating on A tablets consisted mainly of a phthalic alkyd polyester modified with soya oil, whereas the white coating on B tablets contained mostly hydroxypropyl methylcellulose, modified with di-2-ethylhexylphthalate. Some yellow tablets appeared darker than the rest, attributable to the larger film thickness of dark yellow tablets (0.14 mm) compared with that of lighter tablets (0.06 mm). The more than twofold greater film thickness of dark yellow tablets was consistent with the near doubling of disintegration times at pH 6.8, from about 62 minutes for light yellow tablets to about 109 minutes for dark yellow tablets.
Electrostatic Acceleration of Microparticles to Hypervelocities (1960)

By electrostatic methods, μ-diam spheres of iron have been accelerated to hypervelocities. Techniques have been developed to give single impacts in vacuum of measured incident velocity, mass, and position.
End-To-End Interpretable Neural Motion Planner (2019)

In this paper, we propose a neural motion planner for learning to drive autonomously in complex urban scenarios that include traffic-light handling, yielding, and interactions with multiple road-users. Towards this goal, we design a holistic model that takes as input raw LIDAR data and a HD map and produces interpretable intermediate representations in the form of 3D detections and their future trajectories, as well as a cost volume defining the goodness of each position that the self-driving car can take within the planning horizon. We then sample a set of diverse physically possible trajectories and choose the one with the minimum learned cost. Importantly, our cost volume is able to naturally capture multi-modality. We demonstrate the effectiveness of our approach in real-world driving data captured in several cities in North America. Our experiments show that the learned cost volume can generate safer planning than all the baselines.
Cylindrical Compact Thermal-Cycling Device for Continuous-Flow Polymerase Chain Reaction (2003)

A compact, thermal-cycling device for high-throughput continuous-flow polymerase chain reaction (PCR) has been developed, which consists of a flow-through capillary and a cylindrical heating-block assembly. A 3.5-m-long fused-silica capillary coils helically, with 33 turns, up around the 30-mm-diameter assembly of three equally divided thermostating copper blocks for melting, annealing, and extension. An injected PCR mixture undergoes one cycle of PCR each turn. A continuous-flow PCR of one sample and also a segmented-flow PCR of four different samples have been successfully demonstrated. The present device can easily evolve into a parallel-processing, multistation compact device and be modified to have real-time PCR capability. This solid-based compact PCR device, therefore, has a potentiality to be the format of choice when developed for a portable system.
Quickly generating billion-record synthetic databases (1994)

Evaluating database system performance often requires generating synthetic databases—ones having certain statistical properties but filled with dummy information. When evaluating different database designs, it is often necessary to generate several databases and evaluate each design. As database sizes grow to terabytes, generation often takes longer than evaluation. This paper presents several database generation techniques. In particular it discusses: (1) Parallelism to get generation speedup and scaleup. (2) Congruential generators to get dense unique uniform distributions. (3) Special-case discrete logarithms to generate indices concurrent to the base table generation. (4) Modification of (2) to get exponential, normal, and self-similar distributions. The discussion is in terms of generating billion-record SQL databases using C programs running on a shared-nothing computer system consisting of a hundred processors, with a thousand discs. The ideas apply to smaller databases, but large databases present the more difficult problems.
Destruction of Escherichia coli and Salmonella typhimurium in Microwave-cooked Soups (1975)

Single serving (i.e. 200 ml) portions of tomato soup, vegetable soup, and broth inoculated with Escherichia coli or Salmonella typhimurium at about 107 organisms/ml were exposed to 915 MHz microwaves. After various timed exposures the temperature of the top, middle, and bottom regions as indicated by changes in previously positioned assemblies of temperature sensitive paper strips were noted and aliquots were removed from the same regions for standard plate count determination of survivors. For any given exposure time, the temperature of the middle region was warmest; that of the bottom, intermediate; and the temperature of the top, coolest. Despite the relative temperatures of the regions, however, the consistent finding was that, for any exposure time, the closer the sampled organisms were to the top, the lower their level of survival. In terms of temperature, it was noted that organisms in the top had declined to a given level of survival at a temperature lower than the temperature corresponding to the same survival level in the middle or bottom soup regions. These data suggest that the heat generated during the microwave exposure alone is inadequate to fully account for the nature of the lethal effects of microwaves for microorganisms.
Cd47-Signal Regulatory Protein α (Sirpα) Regulates Fcγ and Complement Receptor–Mediated Phagocytosis (2001)

In autoimmune hemolytic anemia (AIHA), circulating red blood cells (RBCs) opsonized with autoantibody are recognized by macrophage Fcgamma and complement receptors. This triggers phagocytosis and elimination of RBCs from the circulation by splenic macrophages. We recently found that CD47 on unopsonized RBCs binds macrophage signal regulatory protein alpha (SIRPalpha), generating a negative signal that prevents phagocytosis of the unopsonized RBCs. We show here that clearance and phagocytosis of opsonized RBCs is also regulated by CD47-SIRPalpha. The inhibition generated by CD47-SIRPalpha interaction is strongly attenuated but not absent in mice with only residual activity of the phosphatase Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1, suggesting that most SIRPalpha signaling in this system is mediated by SHP-1 phosphatase activity. The macrophage phagocytic response is controlled by an integration of the inhibitory SIRPalpha signal with prophagocytic signals such as from Fcgamma and complement receptor activation. Thus, augmentation of inhibitory CD47-SIRPalpha signaling may prevent or attenuate RBC clearance in AIHA.
Molecular Cloning and Expression of a Human Chondroitin Synthase (2001)

We have identified a human chondroitin synthase from the HUGE (human unidentified gene-encoded large proteins) protein data base by screening with two keywords: "one transmembrane domain" and "galactosyltransferase family." The identified protein consists of 802 amino acids with a type II transmembrane protein topology. The protein showed weak homology to the beta1,3-galactosyltransferase family on the amino-terminal side and to the beta1,4-galactosyltransferase family on the carboxyl-terminal side. The expression of a soluble recombinant form of the protein in COS-1 cells produced an active enzyme, which transferred not only the glucuronic acid (GlcUA) from UDP-[(14)C]GlcUA but also N-acetylgalactosamine (GalNAc) from UDP-[(3)H]GalNAc to the polymer chondroitin. Identification of the reaction products demonstrated that the enzyme was chondroitin synthase, with both beta1,3-GlcUA transferase and beta1,4-GalNAc transferase activities. The coding region of the chondroitin synthase was divided into three discrete exons and localized to chromosome 15. Northern blot analysis revealed that the chondroitin synthase gene exhibited ubiquitous but markedly differential expression in the human tissues examined. Thus, we demonstrated that analogous to human heparan sulfate polymerases, the single polypeptide chondroitin synthase possesses two glycosyltransferase activities required for chain polymerization.
Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides (1990)

Non-nucleosidic phosphoramidite linker units suitable for use on commercial DNA synthesis machines have been designed for the direct incorporation of biotin and a new reporter group, phosphotyrosine, at multiple sites on synthetic oligonucleotides. The units are based on a 3-carbon glyceryl backbone where the reporter group is attached to the 2-O-position through a 3-aminopropyl spacer. 17-mer oligonucleotides were synthesized carrying at the 5′-end 1, 2, 4 or 8 biotinyl units or 1, 2, 4 or 8 phosphotyrosinyl units respectively and used for the detection of DNA on nitrocellulose filters by hybridization. Subsequent incubation of the filters with a monoclonal antibody to the reporter group followed by secondary detection using enhanced chemiluminescence (ECL) resulted in amplification of signal strengths as the number of reporter groups was increased. The results were quantitated by use of a charge couple device (CCD) camera. Spacing of biotin moieties by thymidyl residues resulted in further improvements in signal strengths, whereas similar spacing of phosphotyrosinyl units did not.
Digital tomosynthesis in breast imaging. (1997)

Digital mammographic systems make breast tomosynthesis possible. Tomosynthesis may improve the specificity of mammography with improved lesion margin visibility and may improve early breast cancer detection, especially in women with radiographically dense breasts.
Micelles of Diblock Copolymers with Charged and Neutral Blocks:  Scaling and Mean-Field Lattice Approaches (2000)

Polymer micelles composed of block copolymers with one charged and one neutral block in aqueous salt free solution are analyzed by scaling and mean-field lattice theory. We study the micellar structure as a function of the length of the both blocks and the fractional charge of the hydrophilic block. The diagram of the different regimes of the behavior of the equilibrium micellar sizes is constructed. The distribution of the counterions in the system is examined using the scaling and the mean-field approaches. The numerical mean-field results are found to be consistent with three scaling regimes: a quasineutral at small fractional charge, the “Pincus” regime where the Coulomb interaction in the corona is unscreened since the counterions are located outside the corona region, and an osmotic regime where the Coulomb interactions are screened by counterions located in the corona region.
Stereoelectronic factors influencing the biological activity and DNA interaction of synthetic antitumor agents modeled on CC-1065 (1988)

The synthesis, physicochemical properties, and biological activities of a series of novel spiro cyclopropyl compounds, modeled on the potent antitumor antibiotic CC-1065 (1), are described. Many of these synthetic analogues are significantly more effective than 1 against murine tumors. In particular, compound 27 exhibits high activity and potency. Structure-activity analysis supports a molecular mechanism for biological action involving hydrophobic interaction of the drug with DNA and acid-catalyzed alkylation of DNA.
Current Distribution in the Brain From Surface Electrodes (1968)

This work was supported by a NASA Sustaining Grant. Burlington, Vermont* *Department of Electrical Engineering, University of Vermont, Burlington, Vermont 05401. Portions of this paper were presented at the Conference on Effects of Diffuse Electrical Currents on Physiological Mechanisms with Application to Electroanesthesia and Electrosleep, Marquette University, Milwaukee, Wisconsin, October 25–28, 1967.