gebhart/patrepeval huggingface dataset:
Humanized OKT3 antibodies: successful transfer of immune modulating properties and idiotype expression (1992)

Antibodies that possess the Ag-binding regions of OKT3 within the context of a human framework (Hu-OKT3 Ab) offer distinct advantages for optimizing anti-CD3 mAb therapy. First, manipulation of Ab genes to produce humanized Ab that retain Ag-binding activity may circumvent antigenicity problems. Second, Ab gene engineering provides a means for modifying functional properties, including T cell activation and immune suppression. The purpose of this study was to determine the functional properties of Hu-OKT3 Ab and to compare the functional properties and idiotypes of Hu-OKT3 Ab to those of murine OKT3. Three Hu-OKT3 IgG4 Ab, a chimeric OKT3 antibody (cOKT3-1) (grafted sequences comprising all OKT3 VH and VL regions) and two complementarity determining region (CDR)-grafted antibodies, gOKT3-5 and gOKT3-6 (grafted sequences comprising only OKT3 VH and VL CDR and some framework amino acids, were analyzed. Initial studies demonstrated that the cOKT3 and gOKT3-5 Ab bound selectively to T cells and competitively inhibited OKT3-FITC binding with avidities similar to that of murine OKT3. Binding avidity of the gOKT3-6 Ab was markedly less than that of the other two Hu-OKT3 Ab. Serologic analysis suggested that cOKT3 and gOKT3-5 Ab possess idiotypes (combining sites) similar to murine OKT3. T cell activation potency of all three Hu-OKT3 Ab was assessed by proliferation, induction of activation marker expression (IL-2R and Leu 23), and lymphokine production (TNF-alpha and IFN-gamma). The cOKT3 and gOKT3-5 Ab demonstrated T cell activation potencies similar to murine OKT3 as assessed by each parameter. CD3 coating and modulation by these two Ab was effective but somewhat less potent than that observed with OKT3. Finally, cOKT3 and gOKT3-5 Ab both inhibited CTL activity comparably to murine OKT3. In conclusion, these studies indicate that gOKT3-5 and cOKT3 Ab possess immune modulating properties similar to murine OKT3 and thus offer attractive alternatives to murine OKT3 for in vivo therapy.
Synthesis of Indium Amide Compounds (1998)

Indium trichloride reacts with 3 equiv of lithium amide in diethyl ether to give In(NRR')(3) (R = Ph or t-Bu, R' = SiMe(3); R = t-Bu, R' = SiHMe(2)) and with 3 or 4 equiv of LiNMe(SiMe(3)) to yield Li[In{NMe(SiMe(3))}(4)]. The chloride also reacts with LiNPh(2) in THF to give the salt Li[In(NPh(2))(3)Cl] and with LiNRR' in pyridine to yield the neutral adduct In(NRR')(3)(py) (R = R' = Ph; R = Me, R' = SiMe(3)). The volatile liquids In[N(t-Bu)(SiHMe(2))](3) and In[NMe(SiMe(3))](3)(py) react with p-Me(2)Npy to form the solid compounds In[N(t-Bu)(SiHMe(2))](3)(p-Me(2)Npy) and In[NMe(SiMe(3))](3)(p-Me(2)Npy), respectively. X-ray crystallographic studies show that In(NPh(2))(3)(py), In[N(t-Bu)(SiHMe(2))](3)(p-Me(2)Npy), and the ether adduct of In[NPh(SiMe(3))](3) contain nearly planar In(amide)(3) fragments. Crystallographic studies also show that the anion in the salt [Li(THF)(4)][In(NPh(2))(3)Cl] is nearly tetrahedral and in [Li(p-Me(2)Npy)][In{NMe(SiMe(3))}(4)] the tetrahedral-like anion is bound to the Li cation via two amide nitrogens. The Li in the latter structure is also bonded to p-Me(2)Npy, resulting in a planar three-coordinate geometry for Li. Crystal data are the following. C(31)H(52)N(3)OSi(3)In at -50 degrees C: P2(1)/n (monoclinic), a = 11.003(2) Å, b = 18.678(3) Å, c = 17.618(3) Å, beta = 95.42(1) degrees, and Z = 4. C(41)H(35)N(4)In.C(7)H(8) at -50 degrees C: P&onemacr; (triclinic), a = 10.112(2) Å, b = 12.786(3) Å, c = 15.870(5) Å, alpha = 87.42(2) degrees, beta = 74.95(2) degrees, gamma = 78.15(2) degrees, and Z = 2. C(25)H(58)N(5)Si(3)In at -50 degrees C: P2(1)/c (monoclinic), a = 9.797(3) Å, b = 18.203(6) Å, c = 19.592(5) Å, beta = 100.27(2) degrees, and Z = 4. C(52)H(62)ClInLiN(3)O(4) at 23 degrees C: P2(1)/n (monoclinic), a = 16.076(2) Å, b = 17.185(2) Å, c = 18.447(3) Å, beta = 97.41(1) degrees, and Z = 4. C(23)H(58)InLiN(6)Si(4) at 23 degrees C: P&onemacr; (triclinic), a = 15.792(3) Å, b = 16.345(3) Å, c = 16.678(3) Å, alpha = 62.69(1) degrees, beta = 81.00(1) degrees, gamma = 86.94(1) degrees, and Z = 4.
Analyses of Monoglycerides and Other Emulsifiers by Gaschromatography (1983)

Abstract GLC‐analysis has become a still more important tool for the analysis of many emulsifiers and is a powerful supplement to the chemical method for describing an emulsifier. The autosampling technique has made GLC more reproducible which is necessary for quantitative analysis. The new generation of gas chromatographs with computer connection is a good and almost necessary help for calculation of the sample composition and has opened up possibilities for more information. Methods and examples are described for the analysis of monoglyceride, lactic acid esters of monoglyceride, acetic acid esters of monoglyceride, and propylene glycol esters of fatty acids. For more complicated separations of components in emulsifiers capillary columns can be used with good results.
Inhibition of SPHK1 Suppresses Phorbol 12-Myristate 13-Acetate-Induced Metastatic Phenotype in Colorectal Cancer HT-29 Cells (2011)

Expression of sphingosine kinase 1 (SPHK1) plays a role in colorectal cancer progression. This study aimed to demonstrate the mechanism of human colorectal cancer cell metastatic phenotype through SPHK1 knockdown. Human colorectal cancer HT-29 cells were stimulated by phorbol 12-myristate 13-acetate (PMA) with or without SPHK1 siRNA transfection. Tumor cell phenotypic changes were analyzed by using invasion, motility, cell viability, and apoptosis assays. Gene expressions were assessed using Western blot. PMA induced a metastatic phenotype in colorectal cancer cells, as indicated by cell viability, migration and invasion capacity, and ERK1/2 phosphorylation, whereas SPHK1 siRNA transfection suppressed the metastatic phenotype of tumor cells and antagonized PMA's effects. SPHK1 knockdown also inhibited secretion of MMP-2 and MMP-9 into the tumor cell conditioned medium. Suppression of SPHK1 expression suppresses the PMA-induced metastatic phenotype via ERK1/2 phosphorylation in human colorectal cancer cells.
Oligosaccharide units of lysosomal cathepsin D from porcine spleen. Amino acid sequence and carbohydrate structure of the glycopeptides. (1983)

The amino acid sequences near the glycosylation sites and the oligosaccharide structures have been determined for the lysosomal protease cathepsin D from porcine spleen. Cathepsin D light and heavy chains were separately digested with proteases and the glycopeptides were purified. A single sequence was constructed from the amino acid sequence of the light chain glycopeptides which is: Tyr-Asn-Ser-Gly-Lys-Ser-Ser-Thr-Tyr-Val-Lys-Asn(CH2O)-Gly-Thr-Thr-Phe. A single glycopeptide sequence was also obtained for the heavy chain: Lys-Gly-Ser-Leu-Asp-Tyr-His-Asn(CH2O)-Val-Thr-Arg-Lys-Ala-Tyr. The light chain sequence is homologous with the sequence of porcine pepsin from residues 56 to 71. The heavy chain sequence is homologous with the pepsin sequence from residues 176 to 189. Thus, the 2 oligosaccharide-linked asparagines in cathepsin D correspond to residues 67 and 183 in pepsin and other homologous aspartyl proteases. These positions are located on the surface of the crystal structures of aspartyl proteases. Five oligosaccharides linked to Asn-67 were separated and their structures determined with proton NMR. Four major oligosaccharides are structural variants from the high mannose-type having 3, 5, 6, and 7 mannoses, respectively. A minor structure contained a third GlcNAc. Three oligosaccharide structures were found linked to Asn-183. Two major oligosaccharides are of the high mannose-type each with 5 mannose residues. One of the two contains a fucose linked to a GlcNAc. A third, very minor oligosaccharide contains galactose.
Tau–amyloid interactions in the rTgTauEC model of early Alzheimer's disease suggest amyloid‐induced disruption of axonal projections and exacerbated axonal pathology (2013)

Early observations of the patterns of neurofibrillary tangles and amyloid plaques in Alzheimer's disease suggested a hierarchical vulnerability of neurons for tangles, and a widespread nonspecific pattern of plaques that nonetheless seemed to correlate with the terminal zone of tangle-bearing neurons in some instances. The first neurofibrillary cortical lesions in Alzheimer's disease occur in the entorhinal cortex, thereby disrupting the origin of the perforant pathway projection to the hippocampus, and amyloid deposits are often found in the molecular layer of the dentate gyrus, which is the terminal zone of the entorhinal cortex. We modeled these anatomical changes in a transgenic mouse model that overexpresses both P301L tau (uniquely in the medial entorhinal cortex) and mutant APP/PS1 (in a widespread distribution) to examine the anatomical consequences of early tangles, plaques, or the combination. We find that tau uniformly occupies the terminal zone of the perforant pathway in tau-expressing mice. By contrast, the addition of amyloid deposits in this area leads to disruption of the perforant pathway terminal zone and apparent aberrant distribution of tau-containing axons. Moreover, human P301L tau-containing axons appear to increase the extent of dystrophic axons around plaques. Thus, the presence of amyloid deposits in the axonal terminal zone of pathological tau-containing neurons profoundly impacts their normal connectivity.
Simultaneous Deposition of Metallic Bundles of Single-walled Carbon Nanotubes Using Ac-dielectrophoresis (2003)

A simple and scalable scheme based on alternating current (ac-) dielectrophoresis is used for the simultaneous and site-selective deposition of single bundles of single-walled carbon nanotubes (SWNTs) onto a large number of contacts requiring only one bond wire. This allows for a large number of transport measurements that show that the deposited bundles contain at least one metallic SWNT dominating the transport. With high-voltage pulses, metallic bundles are transformed into Schottky-barrier-type field-effect transistors with p-type, ambipolar, or n-type behavior. From a simple electromechanical model, we derive the fact that ac-dielectrophoresis selectively deposits bundles containing metallic tubes.
OC1: randomized induction of oblique decision trees (1993)

This paper introduces OC1, a new algorithm for generating multivariate decision trees. Multivariate trees classify examples by testing linear combinations of the features at each non-leaf node of the tree. Each test is equivalent to a hyperplane at an oblique orientation to the axes. Because of the computational intractability of finding an optimal orientation for these hyperplanes, heuristic methods must be used to produce good trees. This paper explores a new method that combines deterministic and randomized procedures to search for a good tree. Experiments on several different real-world data sets demonstrate that the method consistently finds much smaller trees than comparable methods using univariate tests. In addition, the accuracy of the trees found with our method matches or exceeds the best results of other machine learning methods. 1 Introduction Decision trees (DTs) have been used quite extensively in the machine learning literature for a wide range of classification probl...
Intuitive, Image-Based Cell Sorting Using Optofluidic Cell Sorting (2007)

We present a microfluidic cell-sorting device which augments microscopy with the capability to perform facile image-based cell sorting. This combination enables intuitive, complex phenotype sorting based on spatio-temporal fluorescence or cell morphology. The microfluidic device contains a microwell array that can be passively loaded with mammalian cells via sedimentation and can be subsequently inspected with microscopy. After inspection, we use the scattering force from a focused infrared laser to levitate cells of interest from their wells into a flow field for collection. First, we demonstrate image-based sorting predicated on whole-cell fluorescence, which could enable sorting based on temporal whole-cell fluorescence behavior. Second, we demonstrate image-based sorting predicated on fluorescence localization (nuclear vs whole-cell fluorescence), highlighting the capability of our approach to sort based on imaged subcellular events, such as localized protein expression or translocation events. We achieve postsort purities up to 89% and up to 155-fold enrichment of target cells. Optical manipulation literature and a direct cell viability assay suggest that cells remain viable after using our technique. The architecture is highly scalable and supports over 10 000 individually addressable trap sites. Our approach enables sorting of significant populations based on subcellular spatio-temporal information, which is difficult or impossible with existing widespread sorting technologies.
Recombinant angiopoietin-1 restores higher-order architecture of growing blood vessels in mice in the absence of mural cells (2002)

Interactions between endothelial cells (ECs) and perivascular mural cells (MCs) via signaling molecules or physical contacts are implicated both in vascular remodeling and maintenance of vascular integrity.However, it remains unclear how MCs regulate the morphogenic activity of ECs to form an organized vascular architecture, comprising distinct artery, vein, and capillary, from a simple meshlike network.A clear elucidation of this question requires an experimental model system in which ECs are separated from MCs and yet form vascular structures.Here we report that injection of an antagonistic mAb against PDGFR- into murine neonates provides such an experimental system in the retina by completely blocking MC recruitment to developing vessels.While a vascular network was formed even in the absence of MCs, it was poorly remodeled and leaky.Using this vascular system ideal for direct assessment of the activities of MC-derived molecules, we show that addition of recombinant modified angiopoietin-1 restored a hierarchical vasculature, and also rescued retinal edema and hemorrhage in the complete absence of MCs.These observations demonstrate the potential of Ang1 as a new therapeutic modality for MC dropout in diseases such as diabetic retinopathies.
A Comparison of the Pulmonary Inflammatory Potential of Different Components of Yeast Cell Wall* (2007)

1-->3-Beta-glucan has been associated with pulmonary inflammation induced by exposure to fungal or yeast cell wall dust. 1-->3-Beta-glucan is the major cell wall component of yeast or fungi. However, the yeast cell wall contains several other components besides 1-->3-beta-glucans, such as mannan and chitin. Few studies evaluated the contribution of these other cell wall components to pulmonary inflammation. The present study compares a crude particulate yeast cell wall preparation (zymosan A) to purified yeast glucan, purified yeast glucan mannan, or purified yeast glucan chitin particles for their potency to induce mouse pulmonary inflammation after in vivo exposure. Mannan is the second most abundant polysaccharide in the yeast cell wall, whereas chitin content is a minor component. The results show that pulmonary injury is mediated by both chitin and 1-->3-beta-glucan and to a lesser degree by mannan. There is also evidence that zymosan is more potent than purified 1-->3-beta-glucan alone. Evidence indicates that 1-->3-beta-glucan is the major inflammatory component in yeast and fungal cell walls.
An Examination of Factors Contributing to Critical Thinking and Student Interest in an On-line College-level Art Criticism Course (2007)

This qualitative case study research examined how constructivist problem-based learning facilitated higher level thinking, increased interest in art, and affected attitude toward on-line courses in an undergraduate philosophical aesthetics and interpretation of art criticism course. The research conducted for this study suggests that constructivist problem-based learning does facilitate higher level thinking and increases student interest in art and in on-line classes. Active learning assignments, along with the constructivist collaborative class atmosphere, encouraged students to think more deeply about their personal values concerning art and to consider alternative views. Problem-based learning in this class acted as a scaffold to aid in understanding the material and then in applying the material to unique and real-life situations. Each subject came to the course with certain thinking skills and left with increased knowledge about art but also with increased critical thinking skills for critically examining and discussing art. Participants completed the course with more confidence in their critical thinking ability and in dealing with visual art images. Data was gathered from seven study participants in the form of highly-structured interviews, an early and final critical writing analysis, a major problem assignment and its reflection journal, a beginning survey, and two final surveys. The final major problem involved an individual proposal followed by a collaborative group proposal. Group collaboration constituted the most frustration and problem within the constructivist design of the class. This research took a relativistic viewpoint in gathering data and interpreting meaning.
[Endoscopy of the spine: minimally invasive therapy]. (1992)

Since 1982 discoscopy has proved its value as a mean of continuous optic control of percutaneous intradiscal procedures. Thus, precisely oriented removal of disc tissue became possible for posterior decompression. Since 1989, discoscopy has opened the way for optically controlled intradiscal laser application. Besides decompressive procedures, since 1988 specific elaboration of the vertebral plates for percutaneous interbody fusion has also become available under discoscopic control. A most promising prospect is the technique of foraminoscopy introduced in 1991, which also opened the way to extradiscal exploration. The further development of versatile fine working scopes will be a bigger and bigger challenge to conventional techniques, as the concept of minimal intervention in spine surgery becomes more and more complete.
Phase II clinical trial of Wilms tumor 1 peptide vaccination for patients with recurrent glioblastoma multiforme (2008)

Although a small uncontrolled nonrandomized trial, this study showed that WT1 vaccine therapy for patients with WT1/HLA-A*2402-positive recurrent GBM was safe and produced a clinical response. Based on these results, further clinical studies of WT1 vaccine therapy in patients with malignant glioma are warranted.
Optic Neuritis and Myelitis Following Rubella Vaccination (1982)

Attenuated live virus vaccination provides protection against rubella that is generally safe and effective. Infrequently, however, it is followed by neurologic complications, including paresthesia,<sup>1,2</sup>polyneuritis,<sup>2</sup>myeloradiculoneuritis,<sup>3</sup>and myelitis.<sup>4-7</sup>Kazarian and Gager<sup>8</sup>reported a case of bilateral optic neuritis following trivalent mumps, measles, and rubella vaccination. We describe a patient in whom bilateral optic neuritis and myelitis developed after administration of monovalent rubella vaccine. To the best of our knowledge, optic neuritis has not been previously reported as a complication of this viral vaccine. <h3>REPORT OF A CASE</h3> A 31-year-old woman, in previously excellent health, was found to have no detectable serum rubella antibodies. On Nov 16, 1979, she received an intramuscular attenuated live rubella virus vaccination. Five days later she complained of a headache on the left side and left periorbital pain. During the next several days, she reported pain on movement of her left
High Li+ Conducting Ceramics (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh Li+ Conducting CeramicsHiromichi Aono, Nobuhito Imanaka, and Gin-ya AdachiCite this: Acc. Chem. Res. 1994, 27, 9, 265–270Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September 1994https://pubs.acs.org/doi/10.1021/ar00045a002https://doi.org/10.1021/ar00045a002research-articleACS PublicationsRequest reuse permissionsArticle Views1851Altmetric-Citations166LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Interaction of a transcriptional activator, OmpR, with reciprocally osmoregulated genes, ompF and ompC, of Escherichia coli. (1986)

The ompB locus, comprised of the genes ompR and envZ, regulates the expression of the genes ompF and ompC that encode the major porin proteins in the outer membrane of Escherichia coli K-12. OmpR is believed to activate transcription of ompF and ompC in a reciprocal manner depending upon the osmolarity of the culture medium. We were able to purify OmpR to homogeneity and to characterize its interaction with the porin gene promoters. The purified OmpR was shown to bind specifically to promoter fragments of both ompF and ompC. Deoxyribonuclease I footprinting was carried out in order to determine binding sites of OmpR in the promoter regions of the ompF and ompC. OmpR was found to protect the regions -105 to -60 of ompF and -102 to -78 of ompC, respectively. Two consensus sequences were found in these protected sites and are likely to play an important role in OmpR recognition of these promoter regions. The purified OmpR was also shown to be functionally active in transcription in vitro; OmpR activates the expression of ompF and inhibits the transcription of ompC from the most distal of its three tandem promoters, P3.
Synthesis and cathodoluminescence properties of CdSe/ZnO hierarchical nanostructures (2011)

This report explores the controlled synthesis of ZnO branches on CdSe nanorod (NR) cores. ZnO nanohairs (NHs) grown on the hexagonal planes of CdSe NRs exhibit excellent stoichiometry between their constitute elements. After the growth of ZnO NH branches over the CdSe NRs a quantum confined (QC) cathodoluminescence (CL) UV peak is observed at ∼3.54 eV. From CL studies it is noticed that the dimensions and density of ZnO branches grown on CdSe NR cores have a strong influence on their emission properties. These observations suggest the potentiality of CdSe/ZnO hierarchical structures for future nanostructured optoelectronic device applications.
Nerve Growth Factor Treatment After Brain Injury Prevents Neuronal Death (1987)

Cholinergic neuronal degeneration after axotomy has been proposed to be due to the loss of a retrogradely transported neurotrophic factor, possibly nerve growth factor (NGF). To test this hypothesis, NGF was continuously infused into the lateral ventricles of adult rats that had received bilateral lesions of all cholinergic axons projecting from the medial septum to the dorsal hippocampus. After 2 weeks of NGF treatment, identification of cholinergic neurons by the presence of the biosynthetic enzyme choline acetyltransferase revealed a dramatic increase (350%) in the survival of the axotomized septal cholinergic neurons. Thus, NGF or an NGF-like molecule can act as a neurotrophic factor for these neurons.