gebhart/patrepeval huggingface dataset:
The Effects of Laser and Electron Beam Spot Size in Additive Manufacturing Processes (2017)

In this work, melt pool size in process mapped in power-velocity space for multiple processes and alloys. In the electron beam wire feed and laser powder feed processes, melt pool dimensions are then related to microstructure in the Ti-6Al-4V alloy. In the electron beam wire feed process, work by previous authors that related prior beta grain size to melt pool area is extended and a control scheme is suggested. In the laser powder feed process, in situ thermal imaging is used to monitor melt pool length. Real time melt pool length measurements are used in feedback control to manipulate the resulting microstructure. In laser and electron beam direct metal additive manufacturing, characteristics of the individual melt pool and the resulting final parts are a product of a variety of process parameters. Laser or electron beam spot size is an important input parameter that can affect the size and shape of a melt pool, and has a direct influence on the formation of lack-of-fusion and keyholing porosity. In this work, models are developed to gain a better understanding of the effects of spot size across different alloys and processes. Models are validated through experiments that also span multiple processes and alloys. Methods to expand the usable processing space are demonstrated in the ProX 200 laser powder bed fusion process. In depth knowledge of process parameters can reduce the occurrence of porosity and flaws throughout processing space and allow for the increased use of non-standard parameter sets. Knowledge of the effects of spot size and other process parameters can enable an operator to expand the usable processing space while avoiding the formation of some types of flaws. Based on simulation and experimental results, regions where potential problems may occur are identified and process parameter based solutions are suggested. Methods to expand the usable processing space are demonstrated in the ProX 200 laser powder bed fusion process. In depth knowledge of process parameters can reduce the occurrence of porosity and flaws throughout processing space and allow for the increased use of non-standard parameter sets.
Absolute requirement of CD11/CD18 adhesion molecules, FcRII and the phosphatidylinositol-linked FcRIII for monoclonal antibody-mediated neutrophil antihuman tumor cytotoxicity (1992)

We have previously shown that 3F8, a murine IgG3, monoclonal antibody (MoAb) specific for the ganglioside GD2, mediates tumor cell kill in vitro and in vivo. We now describe receptor requirements of polymorphonuclear leukocytes (PMN) in 3F8-mediated cytotoxicity (ADCC) of human GD2 (+) melanoma and neuroblastoma cell lines. PMN from a child with leukocyte adhesion deficiency (LAD) were devoid of CD11/CD18 adhesion molecules and mounted no detectable ADCC. MoAb to CD11b, CD11c, and CD18 each efficiently blocked ADCC by normal PMN. In contrast, a panel of different MoAbs to CD11a had no significant inhibitory effect on ADCC, a finding consistent with the low-to-absent expression of the CD11a ligand, intercellular adhesion molecule-1, on the target cells. Granulocyte-macrophage colony-stimulating factor (GM-CSF) significantly increased the expression of CD11b, CD11c, and CD18 on normal PMN, decreased the expression of Fc receptors (FcR), and enhanced ADCC by normal but not by LAD PMN. MoAbs to FcRII and FcRIII each efficiently blocked ADCC; anti-FcRI MoAb had no effect. Flow cytometry using anti-FcRII MoAb versus anti-FcRIII MoAb did not show cross competition, suggesting that inhibition of ADCC was not a steric effect resulting from FcRII proximity to FcRIII. PMN deficient in FcRIII (obtained from patients with paroxysmal nocturnal hemoglobinuria) and PMN depleted of FcRIII by treatment with elastase or phosphatidylinositol (PI)-specific phospholipase C produced low ADCC, supporting a role for the PI-liked FcRIII. Thus, optimal ADCC using human PMN, human solid tumor cells, and a clinically active MoAb (conditions that contrast with the heterologous antibodies and nonhuman or nonneoplastic targets used in most models of PMN ADCC) required CD11b, CD11c, FcRII, and the PI-linked FcRIII. Furthermore, in this clinically relevant system, GM-CSF enhancement of antitumor PMN ADCC correlated with increased expression of CD11/CD18 molecules.
Metal oxide photoanodes for solar hydrogen production (2008)

The development of sustainable hydrogen production is a key target in the further facilitation of a hydrogen economy. Solar hydrogen generation through the photolytic splitting of water sensitised by semiconductor materials is attractive as it is both renewable and does not lead to problematic by-products, unlike current hydrogen sources such as natural gas. Consequently, the development of these semiconductor materials has undergone considerable research since their discovery over 30 years ago and it would seem prescient to review the more practical results of this research. Among the critical factors influencing the choice of semiconductor material for photoelectrolysis of water are the band-gap energies, flat band potentials and stability towards photocorrosion; the latter of these points directs us to focus on metal oxides. Careful design of thin films of photocatalyst material can eliminate potential routes of losses in performance, i.e., recombination at grain boundaries. Methods to overcome these problems are discussed such as coupling a photoanode for photolysis of water to a photovoltaic cell in a “tandem cell” device.
Characterization of Nanobubbles on Hydrophobic Surfaces in Water (2007)

The aim of this paper is to quantitatively characterize the appearance, stability, density, and shape of surface nanobubbles on hydrophobic surfaces under varying conditions such as temperature and temperature variation, gas type and concentration, surfactants, and surface treatment. The method we adopt is atomic force microscopy (AFM) operated in the tapping mode. In particular, we show (i) that nanobubbles can slide along grooves under the influence of the AFM tip, (ii) that nanobubbles can spontaneously form by substrate heating, allowing for a comparison of the surface topology with and without the nanobubble, (iii) that a water temperature increase leads to a drastic increase in the nanobubble density, (iv) that pressurizing the water with CO2 also leads to a larger nanobubble density, but typically to smaller nanobubbles, (v) that alcohol-cleaning of the surface is crucial for the formation of surface nanobubbles, (vi) that adding 2-butanol as surfactant leads to considerably smaller surface nanobubbles, and (vii) that flushing water over alcohol-covered surfaces strongly enhances the formation of surface nanobubbles.
The First GPS Satellite Radio Optimized for Automatic Vehicle Location (1991)

A GPS (Global Positioning System) radio designed to serve the needs of the automatic vehicle location (AVL) market is discussed. Market research revealed several key demands for an AVL GPS radio: (i) minimization of urban building blockage; (ii) easy programmability to minimize mobile data transmission costs; (iii) high accuracy for streetmap level coordination; (iv) interface capability with nondigital specialized mobile radios; and (v) selling price close to that of alternatives such as Signposts and Loran-C. The developed system operates at high accuracy in an urban environment and is plug-compatible with nearly all vehicle radios. Among the engineering and production breakthroughs described are a unique approach to satellite tracking, enabling up to 8 GPS satellites to be used for position determination with a two-channel receiver, and a receiver-in-a-microdome design. A powerful application-specific integrated circuit has enabled MARCO to bring GPS within the easy reach of millions of AVL users such as buses, taxis, and delivery vehicle fleets. >
Correcting Sensor Drift and Intermittency Faults With Data Fusion and Automated Learning (2008)

Many fault detection algorithms deal with fault signatures that manifest themselves as step changes. While detection of these step changes can be difficult due to noise and other complicating factors, detecting slowly developing faults is usually even more complicated. Tradeoffs between early detection and false positive avoidance are more difficult to establish. Often times, slow drift faults go completely undetected because the monitoring systems assume that they are ordinary system changes and some monitoring schemes may adapt to the changes. Where redundant sensors are used, a drifting sensor may cause the logic to latch on to the ldquobadrdquo sensor. Another problem may be intermittent sensors faults where the detection logic is too sluggish to recognize a problem before the sensor has returned to seemingly normal behavior. To address these classes of problems, we introduce here a set of algorithms that learns to avoid the bad sensor, thus indirectly recognizing the aberrant sensor. We combine advanced sensor validation techniques with learning. The sensor validation is inspired by fuzzy principles. The parameters of this algorithm are learned using competing optimization approaches. We compare the results from a particle swarm optimization approach with those obtained from genetic algorithms. Results are shown for an application in the transportation industry.
Cloning and nucleotide sequencing of the membrane-bound L-sorbosone dehydrogenase gene of Acetobacter liquefaciens IFO 12258 and its expression in Gluconobacter oxydans (1995)

Cloning and expression of the gene encoding Acetobacter liquefaciens IFO 12258 membrane-bound L-sorbosone dehydrogenase (SNDH) were studied. A genomic library of A. liquefaciens IFO 12258 was constructed with the mobilizable cosmid vector pVK102 (mob+) in Escherichia coli S17-1 (Tra+). The library was transferred by conjugal mating into Gluconobacter oxydans OX4, a mutant of G. oxydans IFO 3293 that accumulates L-sorbosone in the presence of L-sorbose. The transconjugants were screened for SNDH activity by performing a direct expression assay. One clone harboring plasmid p7A6 converted L-sorbosone to 2-keto-L-gulonic acid (2KGA) more rapidly than its host did and also converted L-sorbose to 2KGA with no accumulation of L-sorbosone. The insert (25 kb) of p7A6 was shortened to a 3.1-kb fragment, in which one open reading frame (1,347 bp) was found and was shown to encode a polypeptide with a molecular weight of 48,222. The SNDH gene was introduced into the 2KGA-producing strain G. oxydans IFO 3293 and its derivatives, which contained membrane-bound L-sorbose dehydrogenase. The cloned SNDH was correctly located in the membrane of the host. The membrane fraction of the clone exhibited almost stoichiometric formation of 2KGA from L-sorbosone and L-sorbose. Resting cells of the clones produced 2KGA very efficiently from L-sorbosone and L-sorbose, but not from D-sorbitol; the conversion yield from L-sorbosone was improved from approximately 25 to 83%, whereas the yield from L-sorbose was increased from 68 to 81%. Under fermentation conditions, cloning did not obviously improve the yield of 2KGA from L-sorbose.
The Partial Amino Acid Sequence of Human Myoglobin (1969)

Tryptic and chymotryptic digests of human myoglobin have been separated chromatographically to give 23 pure tryptic peptides and 18 pure chymotryptic peptides. The complete or partial amino acid sequences of these peptides have been established. From the overlaps in sequence between chymotryptic and tryptic peptides it is possible to place 124 of the 152 residues of human myoglobin in a unique sequence. The remaining residues have been ordered tentatively on the basis of the apparent homology in sequence between human and sperm whale myoglobins. Human myoglobin appears to contain 152 residues instead of 153 residues as found in whale myoglobin. The sequence of human myoglobin appears to be accommodated easily into the proposed three-dimensional structure of whale myoglobin, and large differences in the conformation of these two myoglobins cannot be expected.
DNA‐Templated Synthesis in Organic Solvents (2006)

When DNA duplexes are preformed in water, then DNA-templated synthesis (DTS) can take place efficiently and sequence-specifically in organic solvents with low or minimal water content. This simple method enables reactions that are inaccessible in water, such as pyrrolidine-catalyzed aldol condensation, to be performed. The conditions needed to perform DTS in organic solvents also support DNA-templated reactions that are known to take place in aqueous solution. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2006/z500413_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
In situ silicon oxide based intermediate reflector for thin-film silicon micromorph solar cells (2007)

We show that SiO-based intermediate reflectors (SOIRs) can be fabricated in the same reactor and with the same process gases as used for thin-film silicon solar cells. By varying input gas ratios, SOIR layers with a wide range of optical and electrical properties are obtained. The influence of the SOIR thickness in the micromorph cell is studied and current gain and losses are discussed. Initial micromorph cell efficiency of 12.2% (Voc=1.40V, fill factor=71.9%, and Jsc=12.1mA∕cm2) is achieved with top cell, SOIR, and bottom cell thicknesses of 270, 95, and 1800nm, respectively.
Arrayed-waveguide grating multiplexer with flat spectral response (1995)

The design principle of a single-mode arrayed-waveguide grating multiplexer with flat spectral response is proposed on the basis of a discrete Fourier transform. By a beam propagation-method simulation, a flat spectral region (within 1-dB loss increase) is obtained over 57.2 GHz for 100-GHz channel spacing.
Scaling of Chlorosilane SiC CVD to Multi-Wafer Epitaxy System (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Ultrafast Relaxation of Excited Dirac Fermions in Epitaxial Graphene Using Optical Differential Transmission Spectroscopy (2008)

We investigate the ultrafast relaxation dynamics of hot Dirac fermionic quasiparticles in multilayer epitaxial graphene using ultrafast optical differential transmission spectroscopy. We observe differential transmission spectra which are well described by interband transitions with no electron-hole interaction. Following the initial thermalization and emission of high-energy phonons, the electron cooling is determined by electron-acoustic phonon scattering, found to occur on the time scale of 1 ps for highly doped layers, and 4-11 ps in undoped layers. The spectra also provide strong evidence for the multilayer structure and doping profile of thermally grown epitaxial graphene on SiC.
Dectin-2 Is a Pattern Recognition Receptor for Fungi That Couples with the Fc Receptor γ Chain to Induce Innate Immune Responses (2006)

Antigen presenting cells recognize pathogens via pattern recognition receptors (PRR), which upon ligation transduce intracellular signals that can induce innate immune responses. Because some C-type lectin-like receptors (e.g. dectin-1 and DCSIGN) were shown to act as PRR for particular microbes, we considered a similar role for dectin-2. Binding assays using soluble dectin-2 receptors showed the extracellular domain to bind preferentially to hyphal (rather than yeast/conidial) components of Candida albicans, Microsporum audouinii, and Trichophyton rubrum. Selective binding for hyphae was also observed using RAW macrophages expressing dectin-2, the ligation of which by hyphae or cross-linking with dectin-2-specific antibody led to protein tyrosine phosphorylation. Because dectin-2 lacks an intracellular signaling motif, we searched for a signal adaptor that permits it to transduce intracellular signals. First, we found that the Fc receptor gamma (FcRgamma) chain can bind to dectin-2. Second, ligation of dectin-2 on RAW cells induced tyrosine phosphorylation of FcRgamma, activation of NF-kappaB, internalization of a surrogate ligand, and up-regulated secretion of tumor necrosis factor alpha and interleukin-1 receptor antagonist. Finally, these dectin-2-induced events were blocked by PP2, an inhibitor of Src kinases that are mediators for FcRgamma chain-dependent signaling. We conclude that dectin-2 is a PRR for fungi that employs signaling through FcRgamma to induce innate immune responses.
PHOTODYNAMIC SENSITIZERS FROM CHLOROPHYLL:PURPURIN–18 AND CHLORINp6 (1988)

Abstract— Two easily prepared derivatives of chlorophyll,purpurin–18 and chlorin p 6 , are potent sensitizers of cell killing by low‐intensity red light. The internal anhydride group inpurpurin–18 provides the potential of covalently linking in one step the chlorin to cell targeting agents such as antibodies.
An introduction to the multi-user MIMO downlink (2004)

Multiple-input multiple-output (MIMO) communication techniques have been an important area of focus for next-generation wireless systems because of their potential for high capacity, increased diversity, and interference suppression. For applications such as wireless LANs and cellular telephony, MIMO systems will likely be deployed in environments where a single base must communicate with many users simultaneously. As a result, the study of multi-user MIMO systems has emerged recently as an important research topic. Such systems have the potential to combine the high capacity achievable with MIMO processing with the benefits of space-division multiple access. In this article we review several algorithms that have been proposed with this goal in mind. We describe two classes of solutions. The first uses a signal processing approach with various types of transmitter beamforming. The second uses "dirty paper" coding to overcome the interference a user sees from signals intended for other users. We conclude by describing future areas of research in multi-user MIMO communications.
Cleavage specificity of human skin type IV collagenase (gelatinase). Identification of cleavage sites in type I gelatin, with confirmation using synthetic peptides (1990)

Type IV collagenase (gelatinase) readily cleaves denatured collagen into very small peptides. Large cyanogen bromide fragments (25 kDa) of type I collagen are degraded at the same rate as the complete alpha-chain. A number of the gelatinolytic cleavage sites of alpha 1(I)CB7 and alpha 1(I)CB8, representing 50% of the collagen alpha-chain, were determined by sequence analysis of product peptides. In addition to the expected cleavage between glycine and hydrophobic residues, several other cleavage sites were identified. These sites were Gly-Glu, Gly-Asn, and Gly-Ser. Basic residues were found adjacent to the cleavage site in several cases. Hexapeptides containing these unexpected cleavage sites were synthesized, and Km and kcat values were determined. All but one of the Km values were in the submillimolar range, and turnover numbers for the peptides uncharged at the carboxyl terminus were on the order of 10,000/h. Of particular significance was the finding that hydroxyproline occurs 5 residues from the cleavage site in all carboxyl-terminal product peptides and also occurs 5 residues from the cleavage site in seven of nine amino-terminal product peptides. A requirement for hydroxyproline may be of importance in determining the specificity of this enzyme for denatured collagenous substrates.
Expression and functional analysis of recombinant scFv and diabody fragments with specificity for human RhD (2002)

In an attempt to generate recombinant anti-D reagents for possible diagnostic and therapeutic use we cloned the genes encoding the variable (V) domains of a human anti-D antibody secreted by the lymphoblastoid cell line BTSN4. A single-chain Fv (scFv) fragment was constructed using a 21 amino acid linker to join the genes encoding the variable domains of the BTSN4 heavy (VH) and light chains (VL). A diabody construct was also generated by reducing the length of the scFv linker from 21 to 10 residues. The scFv and diabody constructs were cloned into the pFLAG-CTS vector, expressed in E. coli host cells and the recombinant proteins were affinity-isolated from bacterial culture medium. Analysis of the recombinant proteins indicated that they retained the D antigen binding specificity of the parental BTSN4 IgG. Furthermore, both fragments mediated agglutination of papain-treated D positive erythrocytes in the absence of a cross-linking second antibody. While the agglutinating property of BTSN4 diabody was readily explained by the non-covalent association of this protein as a bivalent dimer, oligomeric forms of BTSN4 scFv were not detected when the protein was analysed by size exclusion chromatography. Thus, the agglutinating property of the scFv is not the result of the formation of non-covalently associated multimeric forms of the antibody fragment.
Enhanced Graphics for Teletext (1981)

For more than a decade, the transmission of digital data as part of the television signal has been a subject of study and development by the Independent Broadcasting Authority (IBA). This interest can be traced beyond the first experimental ORACLE transmissions, using a Honeywell DDP-516 mini-computer together with some special interfacing hardware in April 1973, back to the insertion of 'in-house' data on unused television lines in the field interval by an equipment known as SLICE.
Millisecond charge-parity fluctuations and induced decoherence in a superconducting transmon qubit (2013)

The tunnelling of quasiparticles across Josephson junctions in superconducting quantum circuits is an intrinsic decoherence mechanism for qubit degrees of freedom. Understanding the limits imposed by quasiparticle tunnelling on qubit relaxation and dephasing is of theoretical and experimental interest, particularly as improved understanding of extrinsic mechanisms has allowed crossing the 100 microsecond mark in transmon-type charge qubits. Here, by integrating recent developments in high-fidelity qubit readout and feedback control in circuit quantum electrodynamics, we transform a state-of-the-art transmon into its own real-time charge-parity detector. We directly measure the tunnelling of quasiparticles across the single junction and isolate the contribution of this tunnelling to qubit relaxation and dephasing, without reliance on theory. The millisecond timescales measured demonstrate that quasiparticle tunnelling does not presently bottleneck transmon qubit coherence, leaving room for yet another order of magnitude increase.
Topical delivery of paclitaxel for treatment of skin cancer (2016)

The experimental formulations show controlled release of PTX and thus expected to show reduce dose-related side effects.