gebhart/patrepeval huggingface dataset:
Norwalk Virus Vaccines: Challenges and Progress (2000)

Human caliciviruses (HuCVs) are the major cause of outbreaks of acute nonbacterial gastroenteritis throughout the world. An increasing recognition of the clinical significance of these viruses as human pathogens causing foodborne and waterborne disease indicates that an effective vaccine would be useful. This article reviews the current challenges that exist for the development of a vaccine for the HuCVs as well as the status of development of a candidate vaccine. HuCVs are viruses that exhibit a restricted tropism for infection of the gastrointestinal tract of humans, and a volunteer model of infection and disease is available. As pathogens with a restricted host range, the HuCVs are excellent models for understanding the mechanisms that mediate and regulate viral infection of the gastrointestinal tract and mucosal immunity in humans.
Ordering of Human Bone Marrow B Lymphocyte Precursors by Single-Cell Polymerase Chain Reaction Analyses of the Rearrangement Status of the Immunoglobulin H and L Chain Gene Loci (1996)

CD19+CD10+ human B lineage bone marrow cells were separated into cycling or resting cells, which differ in their expression of CD34, VpreB, recombination activating gene (RAG-1), and terminal deoxynucleotidyl transferase (TdT). Polymerase chain reaction analyses developed for DHJH and VkJk, VkJkK(de) and VkK(de) rearrangements with DNA of single cells and a comparison with B lineage cell development in mouse bone marrow, allow to delineate the human B lymphocyte pathway of development as follows: CD34+VpreB+RAG-1+TdT+, DHJH-rearranged, kL germline cycling pre-B I cells-->CD34-VpreB+microH chain+ (pre-B receptor+) RAG-1-TdT-, VHDHJH-rearranged, kL germline, cycling pre-B II cells-->CD34-VpreB-, intracytoplasmic microH chain+ (pre-B receptor-) RAG-1+/-TdT-, VHDHJH-rearranged, mainly kL germline cycling pre-B II cells-->CD34-VpreB-intracytoplasmic microH chain+, RAG-1+TdT-, VHDHJH-rearranged, VkJk-rearranged, IgM-, resting pre-B II cells CD34+VpreB-, sIgM+, RAG-1+TdT-, VHDHJH- and VkJk-rearranged IgM+ immature B cells-->CD34-, CD10-, sIgM+/sIgD+ mature B cells. This order, for the first time established for human B lineage cells, shows striking similarities with that established for mouse B lineage cells in bone marrow.
Validation of Volume Blood Flow Measurements Using Three-Dimensional Distance-Concentration Functions Derived from Digital X-Ray Angiograms (1994)

A practical method for computing highly pulsatile flow waveforms in vivo in tortuous vessels is presented.
Specific regulation of HSPs in human tumor cell lines by flavonoids. (1997)

While the protective role HSPs (Heat Shock Proteins) has been recognized against physiological stress such as heat shock, heavy metals and glucose starvation, recent progress has revealed another aspect of HSPs in various diseases. HSP27 has been shown to be involved in the acquired resistance of tumor cells hyperthermic and chemotherapeutic treatment. In human breast tumors, overexpression of HSP27 is associated with a shorter disease-free survival period. HSP47 is thought to be a collagen specific molecular chaperone. The involvement of HSP47 in the progression of fibrosis has been reported. Aberrant expression of HSP could cause various autoimmune diseases. Manipulation of HSP expression, therefore, could be a therapeutic target to reduce HSP-derived detrimental cellular effects. Flavonoids are a widely distributed group of plant substances, universally present in vascular plants. Although the flavonoids have been known as natural plant products as long as the alkaloids, their pharmacological effects and potential medicinal uses have been little studied by comparison. Today, the picture has changed and the biological and pharmacological activities of plant flavonoids look promising. We investigated the effect of flavonoids on the expression of HSPs in human tumor cell lines. Flavonoids inhibited the expression of HSP27, HSP47, HSP60 and HSP72/73. The results suggested the pharmacological possibilities of flavonoids in diseases derived from abnormal expression of HSPs.
Transfusion-related acute lung injury: epidemiology and a prospective analysis of etiologic factors (2002)

Transfusion-related acute lung injury (TRALI) is a life-threatening complication of hemotherapy. We report a series of 90 TRALI reactions in 81 patients secondary to transfusion with whole blood platelets (72 reactions), apheresis platelets (2), packed red cells (15), and plasma (1). The overall prevalence was 1 in 1120 cellular components. To examine the epidemiology of TRALI, we completed a nested case-control study of the first 46 patients with TRALI compared with 225 controls who had received transfusions. We then completed a prospective analysis of possible biologic response modifiers responsible for 51 of the TRALI cases, including human leukocyte antigen (HLA) class I, class II, and granulocyte antibodies in donors and neutrophil (PMN) priming activity in the plasma of the implicated units and recipients. Two groups were at risk: patients with hematologic malignancies (P <.0004) and patients with cardiac disease (P <.0006). TRALI was associated with older platelets (P =.014). In the prospective study, antileukocyte antibodies were found in only 3.6% of cases. The implicated blood components had greater PMN priming activity than controls (P <.05), and compared with pretransfusion samples, TRALI patients' plasma demonstrated increases in both interleukin 6 (IL-6) and lipid (neutral lipids and lysophosphatidylcholines) priming activity (P <.05). We conclude that TRALI may be more frequent than previously recognized and that patient susceptibility, product age, and increased levels of bioactive lipids in components may predispose patients to TRALI. TRALI, like the acute respiratory distress syndrome, may be a 2-event phenomenon with both recipient predisposition and factors in the stored units playing major roles.
Optically induced rotation of anisotropic micro-objects fabricated by surface micromachining (1994)

Optical trapping and directional high-speed rotation by radiation pressure are demonstrated for anisotropic micro-objects fabricated by reactive ion-beam etching. These micro-objects, which have shape dissymmetry (not bilateral symmetry but rotational symmetry) in the horizontal cross section, rotate about the laser beam axis in the designed direction in a liquid medium (e.g., water or alcohol). The rotation speed is almost proportional to the input laser power.
A Graph-Based Data Model for API Ecosystem Insights (2014)

APIs are increasingly important for companies to enable partners and consumers to access their services and resources. API ecosystems deal with related challenges like publication, promotion and provision of APIs by providers and identification, selection and consumption of APIs by consumers. To address these challenges, to match consumers with relevant APIs, and to support API providers and thus ultimately the ecosystem to evolve, API ecosystems rely on information about APIs, their usage and characteristics, and the social environment around them. We present an extensible, graph-based data model to capture the entities in an API ecosystem and their relations. The data model includes temporal information to capture the evolution of API ecosystems. Analysis operations on top of the data model provide insights for consumers, providers and the ecosystem provider to address the introduced challenges. We present a system implementing the conceptualized data model. We integrate this system with an API ecosystem used in the context of a hackathon event to continuously collect data. We furthermore show the data model's capabilities to represent a well-known dataset about ProgrammableWeb and to drive analysis operations on both datasets.
Trastuzumab emtansine is active on HER-2 overexpressing NSCLC cell lines and overcomes gefitinib resistance (2014)

Our results indicate that targeting HER-2 with T-DM1 may offer a new therapeutic approach in HER-2 over-expressing lung cancers including those resistant to EGFR TKIs.
Low-profile terrorism (2010)

Most of the preventable suffering and death in the world is not caused by terrorism. It is caused by malnutrition, and lack of education, and all the ills connected to poverty. (Martha Nussbaum, in Sterba 2003, p. 248)
Male Gamete Contributions to the Embryo (2005)

During normal fertilization, plasma membranes of a spermatozoon and an oocyte mingle to form a mosaic plasma membrane of a zygote. This may contribute to the polyspermy block of the zygote. Sperm tail components (mitochondria, axonema, and accessory fibers) that enter the oocyte are "digested" without playing major roles in embryo development. The proximal centrosome adjacent to the sperm nucleus may become the center of the sperm aster that brings the male and female pronuclei to the center of the zygote, but it may not be essential for embryonic development per se. Whether sperm RNAs contribute to embryonic development is the subject of controversy. The nucleus is the most important sperm component that enters the oocyte. It is known that 7-15% of the spermatozoa of fertile men are chromosomally abnormal. The proportion of the spermatozoa with subchromosomal abnormalities (including damage at the DNA level) is expected to be even higher. The majority of embryos and fetuses with genomic abnormalities are aborted before reaching term. Structurally abnormal spermatozoa are not necessarily genomically abnormal, even though the incidence of genomically abnormal spermatozoa is higher among structurally abnormal than normal spermatozoa. In mammals, certain genes (estimated to be about 100) in the spermatozoon and oocyte must be "imprinted" in a gender-specific manner to warrant normal embryonic development.
Inhibition of protein synthesis by Cl-. (1977)

Optimum K+ concentration for protein synthesis in four eukaryotic cell-free systems is obtained with 70 to 80 mM added KCl or with 110 to 150 mM added K(OAc). The different K+ optima are due to inhibition of protein synthesis by Cl- at concentrations higher than those present in the cytoplasm of eukaryotic cells. Initiation of protein synthesis is severely inhibited with 150 mM added KCl. This inhibition results from an impairment of mRNA binding to ribosomes. The binding of initiator Met-tRNAt, however, is only slightly inhibited by 150 mM KCl.
trans-dominant inhibition of human hepatitis delta virus genome replication (1991)

Infection with hepatitis delta virus (HDV) is an important cause of acute and chronic liver disease and can be rapidly fatal. Sequencing of the HDV RNA genome has revealed variability at the C-terminal end of the delta antigen reading frame. One genome type (termed the S genome) synthesizes a 24-kDa protein thought to be required for genome replication. Another genome type (termed the L genome) extends the reading frame by 19 amino acids as a result of a single base change. Replication of the S and L genomes was studied in cultured fibroblasts. While the S genome efficiently initiated genome replication, the L genome did not. Moreover, in a codelivery experiment, L genome RNA inhibited replication of the S genome. Potent trans inhibition was also observed following cotransfection of the S genome and a plasmid encoding the larger delta antigen. Mutational analysis indicated that the inhibitory activity was not a simple function of the large delta antigen reading frame's extra length. Implications for the viral life cycle, clinical infection, and potential treatment are discussed.
Effects of endothelial cells and mononuclear leukocytes on platelet aggregation (2000)

Although normal vascular endothelium prevents adhesion and aggregation of platelets by the release of nitric oxide (NO) and prostacyclin, the endothelium exposed to oxidized low density lipoprotein (LDL) may be damaged, damage that may include a reduction in its secretory capacity. On the other hand, mononuclear leukocytes (ML) can also release NO to inhibit platelet aggregation. Platelet aggregation was measured with a Chrono-Log aggregometer (USA) in the presence of cultured endothelial cells (EC) or isolated ML, stimulated by collagen. Platelet aggregation was markedly inhibited (28.5 +/- 5.0 versus control 92.2 +/- 1.7%), when EC were added to platelets. Pre-incubation of the EC with 10 micromol/l of indomethacin or 300 micromol/l of L-NG-monomethyl-arginine (L-NMMA) for 6 h substantially reduced their anti-aggregating activity (72.8 +/- 5.1 and 76.6 +/- 7.7%, respectively). However, incubation of the EC in culture with 10 micromol/l of lysophosphatidyl choline (LPC) for 6 h did not affect the anti-aggregating capacity of EC (LPC 28.1 +/- 5.5 vs. EC 28.5 +/- 5.0%). Non-stimulated ML also inhibited (43.2 + 5.9 vs. control 69.2 3.2%) the platelet aggregation induced by collagen. The inhibition was dependent on the number of ML added. In addition, it was enhanced by increasing the concentration of collagen from 2.5 to 10 microg/ml. Platelet aggregation is inhibited mainly by the EC, however, blood ML also inhibit platelet aggregation. Incubation of the EC in culture with LPC did not affect the anti-aggregating capacity of the EC.
Differentiation of Extrahepatic Bile Duct Cholangiocarcinoma from Benign Stricture: Findings at MRCP versus ERCP (2004)

Accuracy of MRCP is comparable with that of ERCP. Regardless of modality, a lengthy segment of extrahepatic bile duct stricture with irregular margin and asymmetric narrowing suggests cholangiocarcinoma, and a short segment with regular margin and symmetric narrowing suggests benign cause.
In-Vehicle Driver Detection Using Mobile Phone Sensors (2011)

With the advances in smartphone technologies, automated detection and awareness of various user statuses has become a focal point of mobile computing research. In this project, I present a software-based, mobile-phone sensing system to determine if the user is the driver or a passenger in an automobile. The system helps the mobile device’s identify the user’s available attention and focus, enabling it to control delivery of potentially interrupting events such as incoming calls and messages. Since the system does not employ additional hardware, the phone may be carried in different locations and orientations. In addition, the paper will describe the various design challenges, including the evaluation of the system’s energy consumption and operation in noisy environments using the Android 2.3.x and iPhone OS 4.x devices.
Overview: rational integration of agents directed at novel therapeutic targets into combination chemotherapeutic regimens. (2001)

The last decade has witnessed the introduction of a number of agents directed against novel targets into the therapeutic armamentarium for the treatment of cancer. These include agents that interrupt neoplastic cell-survival signaling or cell-cycle regulatory pathways, and differentiation-inducing agents. A common theme linking these agents is that disruption of signal-transduction-, cell-cycle-progression- or differentiation-related pathways frequently leads to mitochondrial damage and apoptosis, and loss of neoplastic cell self-renewal capacity. It has also become apparent that these novel agents may, in a dose- and sequence-dependent manner, lower the threshold for conventional cytotoxic drug-induced apoptosis, resulting in synergistic interactions. More recently, evidence has been accumulating that the judicious combination of agents that interrupt more than one signaling-, cell-cycle-regulatory- or differentiation-related pathway represents an extremely potent apoptotic stimulus for neoplastic cells. The development of rational chemotherapeutic regimens combining these novel agents with others in their class or with conventional cytotoxic drugs presents significant challenges, but offers significant opportunities for therapeutic advances in the treatment of human malignancies. A brief overview of the status of this field follows.
Syntheses and functions of polymers based on amino acids (1999)

In this article, we review our studies concerning the design of novel functional polymers based on amino acids. Although an amino acid is the simplest optically active compound in the nature, its polymers, peptides, and proteins show a wide variety of functions such as electron transfer, information transfer, photo reactivity, and selective catalytic function, which cannot be imitated by synthetic compounds. It is expected to develop “artificial proteins” consisting of polymeric materials which show excellent functions, with exploring a precise molecular design based on amino acids.
The heterogeneity of arginases in rat tissues (1976)

Arginase reactions in rat tissues were shown to be catalysed by three isoenzymes which can be separated by bidirectional electrophoresis on polyacrylamide gels. Anodic electrophoresis reveals a migrating band (isoenzyme I) present in all-non-hepatic tissues except submaxillary gland and a non-migrating band found in all tissues. The latter is resolved by cathodic electrophoresis into isoenzymes III (characteristic of liver and submaxillary gland) and a non-moving band (isoenzyme II), present in kidney, intestine and pancreas. Sequential electrophoresis, in the two directions, of mixture of liver and kidney extracts in the same gel columns separated all three isoenzymes. Differences in the solubilization properties, heat-sensitivity and substrate specificity of arginases from different tissues could be correlated with their electrophoretic behaviour. L-Canavanine could replace arginine as substrate in extracts of kidney but not of liver. Both kidney isoenzymes hydrolysed L-canavanine equally well, whereas isoenzyme III from submaxillary gland showed only very low activity. Antiserum against liver arginase interacted with the enzyme with submaxillary gland, but did not inactivate or adsorb arginase from kidney, intestine or pancreas. The distribution of arginase among 16 normal adult rat tissues is presented; the improved, sensitive, assay method was applicable to tissues containing as little as 0.1% of the hepatic activity.
The Punishment Need Not Fit the Crime: Harmelin v. Michigan and the Eigth Amendment (1993)

datory penalties may be cruel, but they are not unusual in the constitutional sense, having been employed in various forms throughout our Nation's history."'As for the mitigating factors in Harmelin," the Court noted it had "drawn the line of required individualized sentencing at capital cases, and (saw] no basis for extending it further."'This Comment discusses the history of the proportionality guarantee in American jurisprudence in Part II.Part HI describes the facts and procedural history of Harmelin.Part IV critiques the opinions, and attempts to show where proportionality stands after Harmelin.Part V examines Harmelin's effect on proportionality as a component of Eighth Amendment jurisprudence as well as Harmelin's impact on the growing "drug exception" to the Bill of Rights.Part VI concludes that after Harmelin the future of the proportionality principle is in question and, if it does apply to sentences of imprisonment, it will only apply in the most extreme cases. 2 ' II. HISTORICAL BACKGROUNDThe Eighth Amendment of the United States Constitution provides that "[e]xcessive bail shall not be required, nor excessive fines imposed, nor cruel and unusual punishments inflicted." ' The framers of the United States Constitution borrowed the language of the Eighth Amendment directly from the Virginia Constitution of 1776, which derived its language from the English Bill of Rights of 1689.' 18. Id. at
The maize low‐phytic acid 3 encodes a myo‐inositol kinase that plays a role in phytic acid biosynthesis in developing seeds (2005)

Phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate or Ins P6, is the most abundant myo-inositol phosphate in plant cells, but its biosynthesis is poorly understood. Also uncertain is the role of myo-inositol as a precursor of phytic acid biosynthesis. We identified a low-phytic acid mutant, lpa3, in maize. The Mu-insertion mutant has a phenotype of reduced phytic acid, increased myo-inositol and lacks significant amounts of myo-inositol phosphate intermediates in seeds. The gene responsible for the mutation encodes a myo-inositol kinase (MIK). Maize MIK protein contains conserved amino acid residues found in pfkB carbohydrate kinases. The maize lpa3 gene is expressed in developing embryos, where phytic acid is actively synthesized and accumulates to a large amount. Characterization of the lpa3 mutant provides direct evidence for the role of myo-inositol and MIK in phytic acid biosynthesis in developing seeds. Recombinant maize MIK phosphorylates myo-inositol to produce multiple myo-inositol monophosphates, Ins1/3P, Ins4/6P and possibly Ins5P. The characteristics of the lpa3 mutant and MIK suggest that MIK is not a salvage enzyme for myo-inositol recycling and that there are multiple phosphorylation routes to phytic acid in developing seeds. Analysis of the lpa2/lpa3 double mutant implies interactions between the phosphorylation routes.
The Relationship between University and Science and Technology (2002)

By theoretical analysis and detail historical materials, this paper tries to illuminate: that the relationship between university and science and technology could be derived from the 16th century when science and technology was born, while many people thought that they had not relation until the 19th century. Furthermore, after the emergence of medieval university based on scholasticism, it began providing many helps, such as world view and methodology. Therefore, the developing process of relationship between science and technology can be divided into three periods: latent phase, outer phase and definitude phase.
[Treatment of primary and secondary therapy failure in patients with sleep apnea treated with nasal CPAP]. (1991)

Two forms of treatment failure of nCPAP therapy should be distinguished: 1. Primary treatment failure: the reversal of apnoeas and hypopnoeas cannot be achieved due to central SA or a combination of MSA and central hypoventilation. In 10 patients who did not respond to nCPAP (7 patients with purely central SA and 3 patients with MSA who still demonstrated long phases of central hypoventilation during nCPAP) we performed a nasal intermittent positive pressure ventilation (nIPPV) during at least 3 treatment nights. In all patients a reversal of apnoeas and hypopnoeas could be achieved, despite a few phases of mask leakage. Due to possibly life threatening side effects continuous monitoring is required during the initial treatment phase. 8 patients who reported a reduction of symptoms have been successfully using the treatment at home for up to 22 months. 2. Secondary treatment failure: nCPAP leads to a normalisation of breathing pattern but patients refuse nCPAP or discontinue treatment. Besides patients who are poorly motivated there are four major side effects of nCPAP as a reason for non-compliance: a) drying out of the nasopharyngeal mucosa or rhinitis; b) pressure marks caused by the nose mask; c) intolerance to the high expiratory pressure; d) noise. Drying out of the mucosa and rhinitis are the most important side effects. If an increase of room temperature and humidity, nasal cream and small amounts of mint-oil as well as a heat-moisture exchanger (HME) prove inadequate, we use a heated humidifier (Dräger, Aquapor).(ABSTRACT TRUNCATED AT 250 WORDS)