gebhart/patrepeval huggingface dataset:
Expression of neural cell adhesion molecule L1 (CD171) in neuroectodermal and other tumors: An immunohistochemical study of 5155 tumors and critical evaluation of CD171 prognostic value in gastrointestinal stromal tumors (2016)

The neural cell adhesion molecule L1 (CD171) is a multidomain type 1 membrane glycoprotein of the immunoglobulin superfamily important in the nervous system development, kidney morphogenesis, and maintenance of the immune system. Recent studies reported CD171 expression being associated with adverse clinical outcome in different types of cancer and there has been a growing interest in targeting this cell membrane molecule on neoplastic cells by chimeric antigen receptor redirected T lymphocytes or specific antibodies. Nevertheless, conflicting results regarding the prognostic value of CD171 expression in renal cell carcinomas and gastrointestinal stromal tumors were published. In this study, CD171 expression was immunohistochemically analyzed in 5155 epithelial, mesenchymal, melanocytic, and lymphohematopoietic tumors to assess its utility in diagnostic pathology and to pinpoint potential targets for CD171-targeting therapy. A newly developed anti-CD171 rabbit monoclonal antibody, clone 014, was selected from the panel of commercially available CD171 antibodies. Immunohistochemistry was performed using Leica Bond Max automation and multitumor blocks containing up to 60 tumor samples. CD171 was constitutively and strongly expressed in neuroectodermal tumors such as schwannoma, neuroblastoma, and paraganglioma, whereas other mesenchymal tumors including schwannoma mimics showed only rarely CD171 positivity. Frequent CD171-expression was also detected in ovarian serous carcinoma, malignant mesothelioma, and testicular embryonal carcinoma. CD171 immunohistochemistry may have some role in immunophenotypic differential diagnosis of neurogenic tumors and pinpointing potential candidates for anti-CD171 therapy. Though, because of its rare expression and lack of predictive value, CD171 is neither a diagnostic nor prognostic marker for gastrointestinal stromal tumors.
Brain‐Specific Autoantibodies in the Plasma of Subjects with Autistic Spectrum Disorder (2007)

Abstract: Although autism spectrum disorder (ASD) is diagnosed on the basis of behavioral parameters, several studies have reported immune system abnormalities and suggest the possible role of autoimmunity in the pathogenesis of ASD. In this study we sought to assess the incidence of brain‐specific autoantibodies in the plasma of children with autism (AU) compared to age‐matched controls including, siblings without ASD, typically developing (TD) controls, and children with other developmental disabilities, but not autism (DD). Plasma from 172 individuals (AU, n = 63, median age: 43 months; TD controls, n = 63, median age: 48 months; siblings, n = 25, median age: 61 months; and DD controls, n = 21, median age: 38 months) was analyzed by Western blot for the presence of IgG antibodies against protein extracts from specific regions of the human adult brain including the hypothalamus and thalamus. The presence of a ∼ 52 kDa MW band, in the plasma of subjects with AU, was detected with a significantly higher incidence when compared to plasma from TD controls (29% vs. 8%, P = 0.0027 and 30% vs. 11%, P = 0.01, in the thalamus and hypothalamus, respectively). Reactivity to three brain proteins (42–48 kDa MW), in particular in the hypothalamus, were observed with increased incidence in 37% of subjects with AU compared to 13% TD controls ( P = 0.004). Multiple brain‐specific autoantibodies are present at significantly higher frequency in children with AU. While the potential role of these autoantibodies in AU is currently unknown, their presence suggests a loss of self‐tolerance to one or more neural antigens during early childhood.
The matrix metalloproteinase inhibitor batimastat (BB-94) retards human breast cancer solid tumor growth but not ascites formation in nude mice. (1996)

Matrix metalloproteinases (MMPs) are thought to play a significant role in tumor invasion and metastasis as well as angiogenesis. Batimastat, also known as BB-94, acts as an inhibitor of metalloproteinase activity by binding the zinc ion in the active site of MMPs. In our study, the hormone-independent MDA435/LCC6 human breast cancer cell line was used to seed solid tumors s.c. into the region of the mammary fat pad in athymic nude mice. Mice were treated with 50 mg/kg batimastat i.p. Tumor volume measurements showed a statistically significant decrease in tumor size between batimastat-treated and control animals. In contrast, we also used the same MDA435/LCC6 cell line to propagate a malignant ascites in nude mice, which yielded a very different response to batimastat. Batimastat, in previously published literature, had been shown to prolong the life of mice bearing ovarian ascites tumors. Treatment with batimastat in our ascites model produced no increase in survival or significant suppression of ascites formation. However, treated animals showed dramatic tumor cell consolidation and less dispersed ascites cells compared with control animals. Two potential targets of batimastat, gelatinase A and B (MMP-2 and -9, respectively), were examined in both tumor sites. These metalloproteinases were present in both solid tumor and ascites fluid and in both cases were host derived and not produced by the tumor. We conclude that batimastat may have different effects on tumor progression and growth depending on the site of tumor implantation.
Gout (2003)

A 59-year-old man with bilateral olecranon-bursa tophi has frequent bouts of acute gouty arthritis, including three in the past year. Serum uric acid levels are consistently above 9 mg per deciliter. He is moderately obese and has mild, untreated hypertension. Allopurinol was discontinued after a maculopapular rash developed. How should this patient be treated?
Optogenetic Recruitment of Dorsal Raphe Serotonergic Neurons Acutely Decreases Mechanosensory Responsivity in Behaving Mice (2014)

The inhibition of sensory responsivity is considered a core serotonin function, yet this hypothesis lacks direct support due to methodological obstacles. We adapted an optogenetic approach to induce acute, robust and specific firing of dorsal raphe serotonergic neurons. In vitro, the responsiveness of individual dorsal raphe serotonergic neurons to trains of light pulses varied with frequency and intensity as well as between cells, and the photostimulation protocol was therefore adjusted to maximize their overall output rate. In vivo, the photoactivation of dorsal raphe serotonergic neurons gave rise to a prominent light-evoked field response that displayed some sensitivity to a 5-HT1A agonist, consistent with autoreceptor inhibition of raphe neurons. In behaving mice, the photostimulation of dorsal raphe serotonergic neurons produced a rapid and reversible decrease in the animals' responses to plantar stimulation, providing a new level of evidence that serotonin gates sensory-driven responses.
Productive Infection of Plasmacytoid Dendritic Cells with Human Immunodeficiency Virus Type 1 Is Triggered by CD40 Ligation (2002)

Immature plasmacytoid dendritic cells are the principal alpha interferon-producing cells (IPC), responsible for primary antiviral immunity. IPC express surface molecules CD4, CCR5, and CXCR4, which are known coreceptors required for human immunodeficiency virus (HIV) infection. Here we show that IPC are susceptible to and replicate HIV type 1 (HIV-1). Importantly, viral replication is triggered upon activation of IPC with CD40 ligand, a signal physiologically delivered by CD4 T cells. Immunohistochemical staining of tonsil from HIV-infected individuals reveals HIV p24(+) IPC, consistent with in vivo infection of these cells. IPC exposed in vitro to HIV produce alpha interferon, which partially inhibits viral replication. Nevertheless, IPC efficiently transmit HIV-1 to CD4 T-cells, and such transmission is also augmented by CD40 ligand activation. IPC produce RANTES/CCL5 and MIP-1alpha/CCL3 when exposed to HIV in vitro. IPC also induce naïve CD4 T cells to proliferate and would therefore preferentially infect these cells. These results indicate that IPC may play an important role in the dissemination of HIV.
Synchronous and Asynchronous Transfer of Embryos in the Pig (1970)

Estrus and ovulation were controlled by using ICI 33828 and gonadotropin treatment in 52 sexually mature crossbred gilts. Embryos were obtained from donors 4 to 8 days after mating and transferred to recipients at the same phase (synchronous) or at an earlier or later (asynchronous) phase of the estrous cycle. Embryos recovered on days 4 through 8 (day 1=day of HCG injection) were transferred into 45 recipients who had received HCG 1 day previously, the same day or 1 or 2 days later than the donor. When pregnancy resulted from the transfers, embryonic survival at day 30 ranged from 25 to 88%, averaging 50%. Embryonic survival in control animals was 59%. No pregnancies resulted from the transfer of embryos on day 7 or 8. Four of five and three of five recipients were pregnant after transfer of 6-day-old-embryos into day 6 and 4 recipients, respectively. All 10 recipients were pregnant after transfer of 5-day-old-embryos into day 4 and 5 recipients. One of five and three of five recipients were pregnant after transfer of 4-day-old-embryos into day 4 and 5 recipients, respectively. Six of seven control animals remained pregnant, with 50% embryonic survival at day 30. These results indicated that days 5 and 6 were the optimal times for synchronous transfer of embryos as measured by their survival in the recipient at day 30. Days 7 and 8 appeared too late for transferred embryos to survive, and day 4 may have been too early in the cycle for optimal survival. Those transfers in which the donor was 1 or 2 days earlier or 1 day later were as successful as synchronous transfers.
追捕“身份窃贼”（上） (2012)

上月说到我发现查尔斯、露易丝、贾斯汀等几人的关系纠缠不清。案件还得理顺思路，一步一步地侦破。调查过查尔斯的案件后，我发现杀死女友的查尔斯之所以立刻被捕，是因为有人及时报警了。从报警台调出当时的录音，我仔细收听。录音中是一个男子的声音：“我听见隔壁有人大喊大叫，还有打斗的声音，你们最好来查一下……”
Effect of Hepatic Lipase −514C→T Polymorphism and Its Interactions With Apolipoprotein C3 −482C→T and Apolipoprotein E Exon 4 Polymorphisms on the Risk of Nephropathy in Chinese Type 2 Diabetic Patients (2005)

HL T+ genotypes might increase the risk of developing diabetic nephropathy by slowing clearance of triglyceride-rich remnant lipoproteins. In concert with other risk factors (e.g., hyperglycemia), lipid abnormalities may damage the kidneys and endothelium, where reduced binding sites for lipases may precipitate a vicious cycle of dyslipidemia, proteinuria, and nephropathy.
Preferential activation of muscle fibers with peripheral magnetic stimulation of the limb (1989)

Abstract Magnetic and electric activation of limb nerve and muscle were compared in normal subjects of different age, gender, and habitus. Direct stimulation of nerve and muscle showed that activation of intramuscular nerve fibers in the arm and leg occurs at a lower threshold for magnetic stimulation than for electric stimulation. Sensory nerve fibers had a lower threshold with electric stimulation. Muscle activation and stimulus artifact with magnetic stimulation precluded reliable recording of distal sensory nerve action potential in all subjects.
Synthesis of Prolyl Endopeptidase Inhibitors and Evaluation of Their Structure-Activity Relationships: In Vitro Inhibition of Prolyl Endopeptidase from Canine Brain. (1993)

By chemical modification of a known prolyl endopeptidase (PEP) inhibitor (N-[N-(4-phenylbutanoyl)-L-prolyl]pyrrolidine; SUAM-1221), several arylalkanoyl derivatives (V-1--27) were synthesized and tested for in vitro inhibitory activity towards PEP from canine brain. Among them, 4-(2-thienyl)butanoyl derivatives (V-24--27) showed more potent PEP-inhibitory activity than SUAM-1221. The structure-activity relationships of these compounds are discussed.
A marketing model: applications for dietetic professionals. (1986)

Traditionally, dietitians have communicated the availability of their services to the "public at large." The expectation was that the public would respond favorably to nutrition programs simply because there was a consumer need for them. Recently, however, both societal and consumer needs have changed dramatically, making old communication strategies ineffective and obsolete. The marketing discipline has provided a new model and new decision-making tools for many health professionals to use to more effectively make their services known to multiple consumer groups. This article provides one such model as applied to the dietetic profession. The model explores a definition of the business of dietetics, how to conduct an analysis of the environment, and, finally, the use of both in the choice of new target markets. Further, the model discusses the major components of developing a marketing strategy that will help the practitioner to be competitive in the marketplace. Presented are strategies for defining and re-evaluating the mission of the profession, for using future trends to identify new markets and roles for the profession, and for developing services that make the profession more competitive by better meeting the needs of the consumer.
Molecular Detection of Bacterial Pathogens Using Microparticle Enhanced Double-Stranded DNA Probes (2011)

Rapid, specific, and sensitive detection of bacterial pathogens is essential toward clinical management of infectious diseases. Traditional approaches for pathogen detection, however, often require time-intensive bacterial culture and amplification procedures. Herein, a microparticle enhanced double-stranded DNA probe is demonstrated for rapid species-specific detection of bacterial 16S rRNA. In this molecular assay, the binding of the target sequence to the fluorophore conjugated probe thermodynamically displaces the quencher probe and allows the fluorophore to fluoresce. By incorporation of streptavidin-coated microparticles to localize the biotinylated probes, the sensitivity of the assay can be improved by 3 orders of magnitude. The limit of detection of the assay is as few as eight bacteria without target amplification and is highly specific against other common pathogens. Its applicability toward clinical diagnostics is demonstrated by directly identifying bacterial pathogens in urine samples from patients with urinary tract infections.
An Experimental Study on the Curing Method and PP Fiber Mixing Ratio on Spalling Resistance of High Strength Concrete (2009)

본 연구는 양생요인 및 PP 섬유 혼입율 변화에 따른 고강도 콘크리트의 기초적 특성 및 내화특성을 분석하였는데, 그 결과를 요약하면 다음과 같다. 콘크리트의 기초적 특성으로 유동성은 PP 섬유 혼입율이 증가 할수록 감소하는 경향을 나타내었고, 압축강도는 PP 섬유 혼입율 증가에 따라 약간 감소하였으나 재령 7일에서 모두 60 MPa 이상 재령 28일에서 90 MPa이상으로 고강도 범위를 나타내었다. 함수율 변화에 따른 폭렬성상으로 함수율 3.0 %에서 PP 섬유를 혼입하지 않은 플레인을 제외한 모든 배합에서 폭렬이 방지되는 것으로 나타났다. 이때 폭렬이 방지된 경우 잔존압축강도율은 22~41%, 질량감소율 5~7 %로 비교적 양호한 것으로 분석되었다. 양생방법 변화에 따른 폭렬성상으로 표준양생의 경우 PP 섬유 혼입율 0.05 % 이상, 증기 양생 및 오토클레이브의 경우 PP 섬유 혼입율 0.10 %에서 폭렬이 방지되는 것으로 나타났다. 이때 폭렬이 방지된 경우 잔존압축강도율은 23~42 %, 질량감소율은 7~11 % 전후로 나타났다. 이상을 종합하여 볼때 고강도 콘크리트는 함수율이 작을수록 폭렬방지가 용이하고, 양생방법에 따라서는 표준양생이 PP섬유 0.05 % 이상, 증기양생 및 오토클레이브의 경우 PP 섬유 혼입율 0.1 %이상을 혼입해 주어야 폭렬이 방지됨을 알수 있었다. This study is to investigate the fundamental and fireproof qualities of high strength concrete corresponding to changes in the curing factors and the PP fiber ratio. The results were as follows. For the fundamental characteristics of concrete, the fluidity was reduced in proportion to the increase in the PP fiber ratio. The compressive strength was somewhat reduced according to an increase in the PP fiber ratio. However, it had the high strength scope of more than 60 MPa at 7 days and of more than 90 MPa at 28 days. On the spalling mechanism followed by changes of the water content ratio, spalling was prevented in all combinations, except the specimen without PP fiber and subjected to 3.0% of moisture contents. When spalling was prevented at that time, the residual compressive strength ratio was 22%~41% and the mass reduction ratio was 5%~7%, which was relatively favorable. As the spalling mechanism corresponds to changes in the curing method, spalling was prevented in concrete with a PP fiber mixing ratio of more than 0.05% in the event of standard curing, and in concrete with a PP fiber mixing ratio of more than 0.10% in the case of steam curing and autoclave curing. In these cases, when spalling was prevented, the residual compressive strength ratio was 23~42% and the mass reduction ratio was 7~11%. In these results, the ease of spalling prevention in high strength concrete was inversely proportional to the water content ratio. Depending on the curing method, spalling was prevented in concrete with over 0.05% PP fiber with standard curing and in concrete with over 0.1% PP fiber with steam curing and autoclave curing.
Netrin-1 regulates invasion and migration of mouse mammary epithelial cells overexpressing Cripto-1 in vitro and in vivo (2005)

The neuronal guidance molecule, Netrin-1, has been suggested to play a role in the adhesion and migration of the mammary gland epithelium. Human and mouse Cripto-1 induce proliferation, migration, invasion and colony formation by epithelial cells in 3D matrices. Here we investigate whether Netrin-1 affects these Cripto-1-dependent activities in mouse mammary epithelial cells. Overexpression of Cripto-1 in EpH4 and HC-11 cells (EpH4/Cripto-1 or HC-11/Cripto-1) was associated with low expression of Netrin-1 and increased expression of its receptor Neogenin compared to that of wild-type cells. No change was observed in the expression of the other Netrin-1 receptor, UNC5H1. Treating EpH4/Cripto-1 or HC-11/Cripto-1 mammary cells with exogenous soluble Netrin-1 resulted in increased expression of E-cadherin and UNC5H1, decreased expression of vimentin and decreased activation of Akt as determined by western blotting. Colony formation by Eph4/Cripto-1 cells in 3D gels was significantly reduced in proximity to a Netrin-1 source, and mammary glands of transgenic mice overexpressing human Cripto-1 showed altered ductal growth in proximity to implanted Netrin-1-releasing pellets. Terminal end buds in the treated transgenic mice mammary glands also showed increased expression of E-cadherin and UNC5H1 and decreased expression of active Akt determined by immunohistochemistry. Together, these results suggest that regulation of Netrin-1 expression is important in regulating Cripto-1-dependent invasion and migration of mammary epithelial cells.
Distinct roles for PI3K in proliferation and survival of oligodendrocyte progenitor cells (2000)

Phosphoinositol 3-kinase (PI3K) is a downstream effector for multiple ligand-activated receptors and modulates cell responses through activation of its target protein kinase B (Akt). We examined the roles of PI3K-Akt signaling in a primary glial (oligodendrocyte) progenitor cell culture system that is ligand-dependent for cell proliferation, survival, and prevention of differentiation. We demonstrate that PI3K and Akt (Ser-473 phosphorylation) are activated in response to platelet-derived growth factor but not basic fibroblast growth factor-2 (FGF2) and that distinct forms of PI3K are activated in early progenitors and later-maturation pro-oligodendroblasts as identified by their sensitivity to wortmannin. By establishing conditions to examine effects on cell proliferation and survival independently, we demonstrate that PI3K is necessary for a full mitogenic response and that PI3K is also necessary for early progenitor survival. Our results therefore demonstrate that PI3K-Akt signaling independently regulates proliferation and survival, that the form of PI3K is distinct in early progenitors and pro-oligodendroblasts, and that FGF2 does not activate this pathway in either primary glial cell population. J. Neurosci. Res. 62:336–345, 2000. © 2000 Wiley-Liss, Inc.
In Situ Entry of Oligonucleotides into Brain Cells Can Occur through a Nucleic Acid Channel (2007)

Brain tissue has become a challenging therapeutic target, in part because of failure of conventional treatments of brain tumors and a gradually increasing number of neurodegenerative diseases. Because antisense oligonucleotides are readily internalized by neuronal cells in culture, these compounds could possibly serve as novel therapeutic agents to meet such a challenge. In previous in vitro work using cell culture systems, we have demonstrated that intracellular delivery requires a vector such as cationic liposomes since free oligonucleotides remain largely trapped in the endocytic pathway following cellular uptake. Here we studied the cellular uptake properties of oligonucleotides by explants of rat brain (brain slices), and by in vivo brain tissue after administration of oligonucleotides by bolus injection. In contrast to in vitro uptake, we show that in brain slices oligonucleotides were taken up by neuronal and nonneuronal cells, irrespective of their assembly with cationic liposomes. In either case, a diffuse distribution of oligonucleotides was seen in the cytosol and/or nucleus. Uptake of oligonucleotides by brain slices as a result of membrane damage, potentially arising from the isolation procedure, could be excluded. Interestingly, internalization was inhibited following treatment of the tissue with antibody GN-2640, directed against a nucleic acid channel, present in rat kidney cells. Our data support the view that an analogous channel is present in brain tissue, allowing entry of free oligonucleotides but not plasmids. Indeed, for delivery of the latter and accomplishment of effective transfection, cationic lipids were needed for gene translocation into both brain slices and brain tissue in vivo. These data imply that for antisense therapy to become effective in brain, cationic lipid-mediated delivery will only be needed for specific cell targeting but not necessarily for delivery per se to accomplish nuclear deposition of oligonucleotides into brain cells and subsequent down-regulation of disease-related targets.
Lifetimes of quasiparticle excitations in4dtransition metals: Scattering theory and LMTO-RPA-GW approaches (2002)

We report the theoretical studies of electron and hole lifetimes in Nb, Mo, Rh, Pd, Ag by means of an ab initio many-body GW method and of a semiempirical scattering-theory approach (STA). The GW approach includes the evaluation of band structures within the local-density approximation linear muffin-tin orbital TB formalism and employment of random-phase approximation in the calculations of the dielectric functions and screened Coulomb interaction. We show that the density-of-states (DOS) convolution model of the STA provides a good approximation to the ab initio averaged lifetimes, the energy dependence of the matrix element being rather unimportant. We discuss the role of the characteristics of electronic structure responsible for the deexcitation of hot electrons as well as the deviations of the ab initio lifetimes from the predictions of the free-electron gas model and the DOS convolution model.