gebhart/patrepeval huggingface dataset:
Pregnancy-Associated Exosomes and Their Modulation of T Cell Signaling (2006)

Exosome release by viable cells is a feature of activated cell types, including tumors, fetal cells, and cells of the immune system. Exosomes critically regulate immune activation, by mediating activation-induced cell death. Fetal cells may mimic these events to selectively delete reactive lymphocytes. In this study the presence and composition of placenta-derived exosomes are demonstrated in the maternal circulation along with their consequences on T cell activation markers. For all pregnant patients, exosomes were isolated from sera obtained between 28 and 30 wk gestation. For pregnant women, subsequently delivering at term, circulating levels of placental exosomes were 1.8 times greater than those delivering preterm (p < 0.0001). Exosomes isolated from pregnancies subsequently delivering at term expressed significantly higher levels of biologically active components, including Fas ligand (FasL) and HLA-DR, than those from pregnancies delivering preterm. Standardizing for protein concentrations, exosomes from term-delivering pregnancies exhibited greater suppression of CD3-zeta and JAK3 than those delivering preterm. The suppression of CD3-zeta and JAK3 correlated with exosome expression levels of FasL (r2= 0.92 and r2= 0.938, respectively). Fractionation of exosomes from term-delivering pregnancies by continuously eluting electrophoresis indicated that intact 42 kD FasL and an unidentified 24-kDa protein were associated with CD3-zeta suppression. Our results demonstrated that exosomes from pregnancies ultimately delivering at term are present at significantly greater concentrations than those from pregnancies delivering preterm; however, exosomes from term-delivering pregnancies also exhibit significantly greater suppression of CD3-zeta and JAK3.
Gemcitabine, ifosfamide and vinorelbine in advanced non-small cell lung cancer: a phase II study. (2002)

Our results showed that even patients with a poor performance status may benefit from gemcitabine, ifosfamide and vinorelbine treatment, with acceptable toxicity.
FloatingCanvas: quantification of 3D retinal structures from spectral-domain optical coherence tomography (2010)

Spectral-domain optical coherence tomography (SD-OCT) provides volumetric images of retinal structures with unprecedented detail. Accurate segmentation algorithms and feature quantification in these images, however, are needed to realize the full potential of SD-OCT. The fully automated segmentation algorithm, FloatingCanvas, serves this purpose and performs a volumetric segmentation of retinal tissue layers in three-dimensional image volume acquired around the optic nerve head without requiring any pre-processing. The reconstructed layers are analyzed to extract features such as blood vessels and retinal nerve fibre layer thickness. Findings from images obtained with the RTVue-100 SD-OCT (Optovue, Fremont, CA, USA) indicate that FloatingCanvas is computationally efficient and is robust to the noise and low contrast in the images. The FloatingCanvas segmentation demonstrated good agreement with the human manual grading. The retinal nerve fibre layer thickness maps obtained with this method are clinically realistic and highly reproducible compared with time-domain StratusOCT(TM).
Thermal Blanket Injury in the Operating Room (1967)

MODERATE hypothermia is frequently combined with extracorporeal circulation during open heart surgery. At the conclusion of heart-lung bypass the patients are often mildly hypothermic. During the remainder of the operation their body temperatures tend to drift. To maintain normothermia under these conditions, we have on occasion used a thermal blanket on the operating table. Three of our patients treated in this manner have sustained localized skin injuries of significant severity. <h3>Report of Cases</h3><h3>Case 1.</h3> —A 40-year-old white man weighing 90 kg (198.5 1b) had an aortic valve prosthetic replacement for aortic insufficiency. The procedure was uncomplicated and the patient remained on the operating table, and on the thermal blanket, less than four hours. The blanket was at room temperature except when heated to a temperature of 42 C for an estimated period of one hour. Following surgery three linear areas of ischemia corresponding to ridges of the thermal blanket
Myocardium reperfusion injury (2010)

The phenomenon of ischemia/reperfusion injury is described in the experimental models of acute myocardial infarction(AMI),causing additional functional and structural damage to the acute reperfused myocardium,and ischemic preconditioning refers to the myocardial ischemia after a long period of reperfusion before one or several shortoccasional duplication of myocardial ischemia /reperfusion1,which can increase myocardial ischemic tolerance.The therapeutic strategies for AMI have focused on myocardial ischemia /reperfusion injury,which accounts for a significant part of the final infarct size.Although experiments in the last 20 years have reported that pharmacological interventions at reperfusion might reduce myocardial reperfusion injury,this could not be confirmed in human studies.An alternative to chemical modifiers,postconditioning(brief repeated periods of ischemia applied at the onset of reperfusion) is another method proven to be efficient in animal models and to be confirmed in recent human studies.This simple method,applied in the first minute of reperfusion,reduces the final infarct size by 30%-50%.This review will focus on the mechanisms,pharmacological preconditioning,postconditioning technique,which is easily applicable in human patients in the setting of AMI.
Upper bound on peak factor of N-multiple carriers (1997)

An upper bound on the peak factor of an N carrier orthogonal frequency division multiplexing (OFDM) is derived. This bound highlights the intimate relationship between the peak factor and the out-of-phase aperiodic autocorrelation values of the data sequence.
Face sketch synthesis and recognition (2003)

We propose a novel face photo retrieval system using sketch drawings. By transforming a photo image into a sketch, we reduce the difference between photo and sketch significantly, thus allow effective matching between the two. To improve the synthesis performance, we separate shape and texture information in a face photo, and conduct transformation on them respectively. Finally a Bayesian classifier is used to recognize the probing sketch from the synthesized pseudo-sketches. Experiments on a data set containing 606 people clearly demonstrate the efficacy of the algorithm.
Controls and Diagnostics for Air Distribution Systems (2003)

Fault detection and diagnostics applied to heating, ventilating, and air-conditioning (HVAC) equipment has been an active area of research over the past decade, with much of the work focusing on air distribution systems. Concurrent efforts have sought to improve the control of these systems. This paper discusses the relationship between controls and diagnostics for air distribution systems, provides an overview of the diagnostic literature for these systems, presents new findings from a diagnostic method that enables operational characteristics of individual HVAC components to be extracted from high-frequency measurements of whole-building power, and outlines research challenges that remain to be addressed.
3D seismic attributes for a tight gas sand reservoir characterization of the eastern Sulige gas field, Ordos Basin, China (2015)

ABSTRACT The Lower Permian Xiashihezi Formation of the Ordos Basin is the largest producer of tight gas sand in China. The controls on tight gas production are many and include a variety of geologic, hydrodynamic, and engineering factors from one well to another throughout the basin. In this study, we considered data from a 408 km2 3D seismic volume and logs from 17 wells to investigate the geologic controls on gas production in the P1h8 member of the Xiashihezi Formation, eastern Sulige gas field, Ordos Basin. Our objective was to determine the potential of applying multiple seismic attributes to identify the higher productivity areas of a tight gas sand reservoir. To achieve this, we used amplitude, complex traces, spectral decomposition, and seismic attenuation attributes derived from the 3D seismic volume to detect gas-bearing sand areas. The results of seismic attribute analysis revealed that no single attribute is correlated to higher productivity areas. The qualitative correlations between attributes and production records reflected that higher productivity areas are associated with seismically definable higher amplitude, more stable phase, tuning frequency, and stronger attenuation features in the study area. Meanwhile, three outlier wells in the seismic attribute analysis provided a reminder of the uncertainty in geologic interpretation. The gas-sand reservoir evaluation results suggested that the Pareto principle helps to enhance the interpretation needed to determine the productivity distribution of P1h8 tight-gas reservoir in the study area.
NF-κB Controls Cell Growth and Differentiation through Transcriptional Regulation of Cyclin D1 (1999)

Accumulating evidence implicates the transcription factor NF-kappaB as a positive mediator of cell growth, but the molecular mechanism(s) involved in this process remains largely unknown. Here we use both a skeletal muscle differentiation model and normal diploid fibroblasts to gain insight into how NF-kappaB regulates cell growth and differentiation. Results obtained with the C2C12 myoblast cell line demonstrate that NF-kappaB functions as an inhibitor of myogenic differentiation. Myoblasts generated to lack NF-kappaB activity displayed defects in cellular proliferation and cell cycle exit upon differentiation. An analysis of cell cycle markers revealed that NF-kappaB activates cyclin D1 expression, and the results showed that this regulatory pathway is one mechanism by which NF-kappaB inhibits myogenesis. NF-kappaB regulation of cyclin D1 occurs at the transcriptional level and is mediated by direct binding of NF-kappaB to multiple sites in the cyclin D1 promoter. Using diploid fibroblasts, we demonstrate that NF-kappaB is required to induce cyclin D1 expression and pRb hyperphosphorylation and promote G(1)-to-S progression. Consistent with results obtained with the C2C12 differentiation model, we show that NF-kappaB also promotes cell growth in embryonic fibroblasts, correlating with its regulation of cyclin D1. These data therefore identify cyclin D1 as an important transcriptional target of NF-kappaB and reveal a mechanism to explain how NF-kappaB is involved in the early phases of the cell cycle to regulate cell growth and differentiation.
Bioavailability of Citrus Polymethoxylated Flavones and Their Biological Role in Metabolic Syndrome and Hyperlipidemia (2012)

Bioavailability of Citrus Polymethoxylated Flavones and Their Biological Role in Metabolic Syndrome and Hyperlipidemia
Design and Kinematic Modeling of Constant Curvature Continuum Robots: A Review (2010)

Continuum robotics has rapidly become a rich and diverse area of research, with many designs and applications demonstrated. Despite this diversity in form and purpose, there exists remarkable similarity in the fundamental simplified kinematic models that have been applied to continuum robots. However, this can easily be obscured, especially to a newcomer to the field, by the different applications, coordinate frame choices, and analytical formalisms employed. In this paper we review several modeling approaches in a common frame and notational convention, illustrating that for piecewise constant curvature, they produce identical results. This discussion elucidates what has been articulated in different ways by a number of researchers in the past several years, namely that constant-curvature kinematics can be considered as consisting of two separate submappings: one that is general and applies to all continuum robots, and another that is robot-specific. These mappings are then developed both for the single-section and for the multi-section case. Similarly, we discuss the decomposition of differential kinematics (the robot’s Jacobian) into robot-specific and robot-independent portions. The paper concludes with a perspective on several of the themes of current research that are shaping the future of continuum robotics.
Theoretical study of environmental dependence of oxygen vacancy formation in CeO2 (2005)

Density functional methods were used to study the environmental dependence of O vacancy formation in CeO2. It was found that an O vacancy in the 2+ charged state has the lowest formation energy for a wide range of Fermi energies (EF) from 0 to ∼1.9eV, while a neutral vacancy becomes the most stable at higher EF values. The O vacancy formation energy can be strongly affected by temperature (T) and the partial pressure of oxygen (PO2). The effect of T and PO2 on the equilibrium compositions of reduced ceria (CeO2−x) was also calculated and showed qualitative agreement with gravimetric experiments.
Soluble CD14 acts as a shuttle in the neutralization of lipopolysaccharide (LPS) by LPS-binding protein and reconstituted high density lipoprotein. (1995)

We have recently shown that lipopolysaccharide (LPS)-binding protein (LBP) is a lipid transfer protein that catalyzes two distinct reactions: movement of bacterial LPS (endotoxin) from LPS micelles to soluble CD14 (sCD14) and movement of LPS from micelles to reconstituted high density lipoprotein (R-HDL) particles. Here we show that LBP facilitates a third lipid transfer reaction: movement of LPS from LPS-sCD14 complexes to R-HDL particles. This action of LBP is catalytic, with one molecule of LBP enabling the movement of multiple LPS molecules into R-HDL. LBP-catalyzed movement of LPS from LPS-sCD14 complexes to R-HDL neutralizes the capacity of LPS to stimulate polymorphonuclear leukocytes. Our findings show that LPS may be transferred to R-HDL either by the direct action of LBP or by a two-step reaction in which LPS is first transferred to sCD14 and subsequently to R-HDL. We have observed that the two-step pathway of LPS transfer to R-HDL is strongly favored over direct transfer. Neutralization of LPS by LBP and R-HDL was accelerated more than 30-fold by addition of sCD14. Several observations suggest that sCD14 accelerates this reaction by serving as a shuttle for LPS: addition of LBP and sCD14 to LPS micelles resulted in LPS-sCD14 complexes that could diffuse through a 100-kD cutoff filter; LPS-sCD14 complexes appeared transiently during movement of LPS to R-HDL facilitated by purified LBP; and sCD14 could facilitate transfer of LPS to R-HDL without becoming part of the final LPS-R-HDL complex. Complexes of LPS and sCD14 were formed transiently when LPS was incubated in plasma, suggesting that these complexes may play a role as intermediates in the neutralization of LPS under physiological conditions. These findings detail a new activity for sCD14 and suggest a novel mechanism for lipid transfer by LBP.
Molecular cloning of protein 4.1, a major structural element of the human erythrocyte membrane skeleton. (1986)

Protein 4.1 is an important structural protein that is expressed in erythroid and in a variety of non-erythroid tissues. In mammalian erythrocytes, it plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. We report here the molecular cloning and characterization of human erythrocyte protein 4.1 cDNA and the complete amino acid sequence of the protein derived from the nucleotide sequence. Probes prepared from the cloned erythrocyte protein 4.1 cDNA hybridized with distinct mRNA species from a wide variety of non-erythroid tissues, including brain, liver, placenta, pancreas, and intestine, implying substantial homology between erythroid and non-erythroid protein 4.1. The availability of cloned erythrocyte protein 4.1 cDNA should facilitate the study of the functional characteristics of this protein in erythroid as well as non-erythroid cells.
3D rotation estimation using discrete spherical harmonic oscillator transforms (2014)

This paper presents an approach to 3D rotation estimation using discrete spherical harmonic oscillator transforms (discrete SHOTs). Discrete SHOTs not only have simple and fast implementation methods but also are compatible with the existing angle estimation algorithms related to spherical harmonics. Discrete SHOTs of the rotated signal follow the same formulation to the Wigner-D matrix as spherical harmonics transforms. Thus, the spherical harmonics related algorithms could be utilized to discrete SHOTs without modification. Furthermore, compared to some existing methods, our approach with discrete SHOTs exhibits higher accuracy, higher precision and improved robustness to noise if the input signal is sampled uniformly on Cartesian grids. The phenomenon results from no interpolations in discrete SHOTs.
Direction-oriented interpolation and its application to de-interlacing (2003)

A new direction-oriented interpolation method is proposed and then applied to de-interlacing. The proposed method introduces the upper spatial direction vector. (USDV) and the lower spatial direction vector (LSDV) to obtain more accurate direction of the highest spatial correlation. Using the USDV and the LSDV, the proposed method reduces possibility of wrong decision for the highest-correlated spatial direction. Line-based directional interpolation is performed after the direction vector is found. Extensive simulations conducted for images and video sequences show the efficacy of the proposed method over the previous methods based on the ELA and over the line averaging method in terms of the objective and subjective image quality.
Oxytocin synthesis and oxytocin receptor expression by cell lines of human small cell carcinoma of the lung stimulate tumor growth through autocrine/paracrine signaling. (2002)

The objective of the present work was to investigate the existence of an oxytocin (OT)-mediated autocrine/paracrine signaling upon small cell carcinoma of the lung (SCCL) cell growth. In that view, OT receptor (OTR) expression, concomitant with OT synthesis and secretion, was evidenced on three different SCCL cell lines (DMS79, H146, and H345) and related to the vasopressin (VP) system. Specific OT, VP, OTR, V1a VP receptor (V1aR), and V1b/V3 VP receptor (V1bR/V3R) transcripts were identified by reverse transcription-PCR in all cell lines studied. Binding of 125I-(d(CH2)(5)(1), Tyr(Me)(2),Thr(4),Orn(8),Tyr(9)-NH2)-vasotocin (OVTA) was observed on all SCCL cell lines, with a K(d) (dissociation constant) ranging from 0.025-0.089 nM, depending on the cell line and the analytical method. Selectivity of 125I-OVTA binding was confirmed by displacement curves obtained with various OTR and VP receptor agonists and antagonists (OT, OVTA, L-371,257, VP, F180). Immunocytochemistry identified cellular OT and VP, and peptide secretion was measured in supernatants of SCCL cultures. [3H]Thymidine incorporations, applied on H345 cells, demonstrated a dose-dependent mitogenic effect of exogenous OT (1 and 100 nM) that was abolished by the OTR antagonist OVTA. A decrease of proliferation was also observed with OVTA alone, showing a functional mitogenic effect of tumor-derived OT. Taken together, these observations demonstrate the existence of a functional OT-mediated autocrine/paracrine signaling actively implicated in growth and development of SCCL tumors. Furthermore, these findings point to the potential of OT antagonists for development as therapeutic agents for the treatment of SCCL.
Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice (2015)

Structural variations (SVs) contribute to the variability of our genome and are often associated with disease. Their study in model systems was hampered until now by labor-intensive genetic targeting procedures and multiple mouse crossing steps. Here we present the use of CRISPR/Cas for the fast (10 weeks) and efficient generation of SVs in mice. We specifically produced deletions, inversions, and also duplications at six different genomic loci ranging from 1.1 kb to 1.6 Mb with efficiencies up to 42%. After PCR-based selection, clones were successfully used to create mice via aggregation. To test the practicability of the method, we reproduced a human 500 kb disease-associated deletion and were able to recapitulate the human phenotype in mice. Furthermore, we evaluated the regulatory potential of a large genomic interval by deleting a 1.5 Mb fragment. The method presented permits rapid in vivo modeling of genomic rearrangements.
Single Dose and Repeat Once-Daily Dose Safety, Tolerability and Pharmacokinetics of Valbenazine in Healthy Male Subjects (2025)

Valbenazine (VBZ) is a vesicular monoamine transporter 2 (VMAT2) inhibitor approved for the treatment of tardive dyskinesia. The safety, tolerability and pharmacokinetics of VBZ following single and repeat once-daily (QD) dosing were evaluated in 2 randomized, single-center, double-blind studies in healthy male subjects. In the first study, 2 cohorts of 8 subjects were administered single doses (SD) of placebo (PBO; N = 2/period) or VBZ (N = 6/period; 1, 2, 5, or 12.5 mg for Cohort 1 and 12.5, 25, 50, or 75 mg for Cohort 2) using a sequential escalation scheme. The second study consisted of 2 phases. In the initial phase, subjects were administered SD PBO (N = 2/period) or VBZ (N = 6/period; 75, 100, 125 or 150 mg) with sequential escalation. In the second phase, subjects received PBO, or 50 or 100 mg VBZ (N = 4:8:8) QD for 8 days (Cohort 1) or PBO or 50 mg VBZ (N = 6:6) QD for 8 days (Cohort 2). For both studies, plasma concentrations of VBZ and its active metabolite, NBI-98782, were determined. Safety was assessed throughout the studies. PK parameters were determined using noncompartmental methods. In both studies, VBZ was rapidly absorbed with peak concentrations typically observed within 1.5 hours. Peak NBI-98782 concentrations were typically observed at 4.0 to 9.0 hours. Terminal elimination half-life for both VBZ and NBI-98782 was ~20 hours. Across the 1 to 150 mg SD range evaluated across the studies, VBZ and NBI-98782 C<sub>max</sub> and AUC increased dose-proportionally from 50 to 150 mg and more than dose-proportionally from 1 to 50 mg. QD VBZ and NBI-98782 C<sub>max</sub> and AUC parameters were also dose-proportional between the 50 and 100 mg doses. Steady-state for both analytes appeared to be achieved by Day 8. The accumulation index was ~1.5 for VBZ and ~2.5 for NBI-98782. Peak to trough fluctuation was approximately 250% for VBZ and 70% for NBI-98782. Across both studies, NBI-98782 exposure was approximately 20%-30% that of VBZ based on molar ratios. In the first study, the maximum-tolerated dose was not achieved; headache (2 events) was the only treatment-emergent adverse event (TEAE) reported by more than one subject. In the second study, fatigue (4 events) was the only TEAE reported by more than one subject following SD VBZ. Following QD VBZ, the TEAEs of fatigue, insomnia, disturbance in attention, and nervousness were dose-dependent; the latter three TEAEs were considered dose-limiting. Subject withdrawals due to TEAEs were 1 each for PBO and 50 mg VBZ QD, and 3 for 100 mg VBZ QD. Clinically relevant effects on laboratory parameters, vital signs or ECGs were limited to increased CPK (SD: 1 each for 5 mg VBZ and PBO), ALT (QD: 1 each for 50 and 100 mg VBZ and PBO), and triglycerides (QD: 1 each for 50 mg VBZ and PBO). VBZ has an acceptable safety profile and predictable pharmacokinetics that result in stable concentrations of active compounds with low peak-to-trough fluctuation following once-daily dosing.
Case 20-2002 (2002)

Case Records of the Massachusetts General Hospital from The New England Journal of Medicine — Case 20-2002 — A 37-Year-Old Man with Fever, Hepatosplenomegaly, and a Cutaneous Foot Lesion after a Trip to Africa