gebhart/patrepeval huggingface dataset:
Efficacy of Electrolyzed Oxidizing Water for Inactivating Escherichia coli O157:H7, Salmonella enteritidis , and Listeria monocytogenes (1999)

The efficacy of electrolyzed oxidizing water for inactivating Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes was evaluated. A five-strain mixture of E. coli O157:H7, S. enteritidis, or L. monocytogenes of approximately 10(8) CFU/ml was inoculated in 9 ml of electrolyzed oxidizing water (treatment) or 9 ml of sterile, deionized water (control) and incubated at 4 or 23 degrees C for 0, 5, 10, and 15 min; at 35 degrees C for 0, 2, 4, and 6 min; or at 45 degrees C for 0, 1, 3, and 5 min. The surviving population of each pathogen at each sampling time was determined on tryptic soy agar. At 4 or 23 degrees C, an exposure time of 5 min reduced the populations of all three pathogens in the treatment samples by approximately 7 log CFU/ml, with complete inactivation by 10 min of exposure. A reduction of >/=7 log CFU/ml in the levels of the three pathogens occurred in the treatment samples incubated for 1 min at 45 degrees C or for 2 min at 35 degrees C. The bacterial counts of all three pathogens in control samples remained the same throughout the incubation at all four temperatures. Results indicate that electrolyzed oxidizing water may be a useful disinfectant, but appropriate applications need to be validated.
Pyrazole addition to Δ1-azirines. Structure of purported 2:1 adducts of Δ1-azirines with sym-tetrazines (1974)

The previously unestablished structure of purported 2:1 adducts isolated from the reaction of Δ1-azirines with s-tetrazines is now shown to be identical with the aziridine derivatives resulting from thermally promoted addition of pyrazoles to Δ1-azirines.
Addition of Fibronectin to Alginate Matrix Improves Peripheral Nerve Regeneration in Tissue-Engineered Conduits (2003)

Schwann cell (SC) transplantation has been proposed to encourage peripheral nerve regeneration, but an optimal SC-carrying matrix would be needed. The aim of this study was to characterize how the addition of fibronectin to alginate would affect the outcome of nerve regeneration promoted by Schwann cells embedded in this matrix. Genetically labeled rat SCs were obtained by lacZ gene transduction. SCs were suspended in alginate hydrogel matrix with/without addition of liquid fibronectin, and their viability and growth in the different types of matrices were assessed in vitro by AlamarBlue assay. In vivo assessment of SC transplantation in the matrix was carried out with poly-3-hydroxybutyrate (PHB) conduits to bridge a sciatic nerve gap. The grafted conduits were harvested at 2, 3, and 6 weeks and assessed for the presence of labeled SCs in relation to regrowing axons. The amount and rate of axonal regeneration were assessed by quantitative immunohistochemistry. Addition of fibronectin to alginate hydrogel improved SC viability and growth profile in vitro. X-Gal staining confirmed that SCs transplanted in PHB conduits were viable throughout the time course, and that the labeled SCs were clearly associated with regenerating axons. The regeneration rate was enhanced when liquid fibronectin was added to the alginate matrix. Furthermore, the presence of SCs also enhanced regeneration and there was an additive effect when both SCs and fibronectin were combined with alginate. In conclusion, the addition of fibronectin to alginate hydrogel matrix contributed to improve nerve regeneration, supporting SC viability and augmenting their effect on axonal growth when transplanted in a bioengineered nerve conduit.
Enhancing reality in the operating room (2002)

Three dimensional computer models of the anatomy generated from volume acquisitions of computed tomography and magnetic resonance imaging are useful adjuncts to 2D images. This paper describes a system that merges the computer generated 3D models with live video to enhance the surgeon's understanding of the anatomy beneath the surface. The system can be used as a planning aid before the operation and provide additional information during an operation. The application of the system to a brain operation is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Antigen‐specific targeting of CD28‐mediated T cell co‐stimulation using chimeric single‐chain antibody variable fragment‐CD28 receptors (1996)

T cells require two distinct signals for optimal activation. One is an antigen-specific signal and is provided by engagement of the T cell receptor (TCR). The second is an antigen-independent signal mediated by engagement of the T cell surface molecule CD28 with members of the B7 family. To endow CD28 molecules with antibody-type recognition, we have constructed chimeric single-chain antibody variable fragment (scFv)-CD28 molecules; following transfection of the genes encoding such constructs into the Jurkat human T cell line we show that they are stably expressed as functional cell surface receptors. These chimeric molecules have no apparent negative effects on the expression and signaling ability of the wild-type CD28 and TCR/CD3 molecules. When combined with signaling via the TCR/CD3 complex, these antigen-specific scFv-CD28 chimeric molecules provide signals similar to those elicited by the cross-linking of the unmodified CD28 molecules. Furthermore, the generation of double transfectants simultaneously expressing scFv-CD28 and scFv-CD3 zeta chimeras demonstrates that antigen-specific co-stimulatory signals can also synergize with signals mediated through chimeric CD3 zeta chains to secrete maximal levels of interleukin-2. Overall, our results suggest that optimal, predefined antigen-specific activation of T cells directed by the specificity of the scFv should be possible.
Oxidative stress in patients with Friedreich ataxia (2000)

Increased generation of reactive oxygen species may underlie the pathophysiology of Friedreich ataxia (FRDA). The authors measured concentrations of 8-hydroxy-2'-deoxyguanosine (8OH2'dG), a marker of oxidative DNA damage, in urine and of dihydroxybenzoic acid (DHBA), a marker of hydroxyl radical attack, in plasma of 33 patients with FRDA. They found a 2.6-fold increase in normalized urinary 8OH2'dG but no change in plasma DHBA as compared with controls. Oral treatment with 5 mg/kg/day of the antioxidant idebenone for 8 weeks significantly decreased urinary 8OH2'dG concentrations, indicating that 8OH2'dG may be useful in monitoring therapeutic interventions in patients with FRDA.-1721
Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia (2004)

Impaired magnesium reabsorption in patients with TRPM6 gene mutations stresses an important role of TRPM6 (melastatin-related TRP cation channel) in epithelial magnesium transport. While attempting to isolate full-length TRPM6, we found that the human TRPM6 gene encodes multiple mRNA isoforms. Full-length TRPM6 variants failed to form functional channel complexes because they were retained intracellularly on heterologous expression in HEK 293 cells and Xenopus oocytes. However, TRPM6 specifically interacted with its closest homolog, the Mg(2+)-permeable cation channel TRPM7, resulting in the assembly of functional TRPM6/TRPM7 complexes at the cell surface. The naturally occurring S141L TRPM6 missense mutation abrogated the oligomeric assembly of TRPM6, thus providing a cell biological explanation for the human disease. Together, our data suggest an important contribution of TRPM6/TRPM7 heterooligomerization for the biological role of TRPM6 in epithelial magnesium absorption.
Pharmaceutical Substances: Syntheses, Patents, Applications (1999)

Pharmaceutical Substances is the newly revised and expanded edition of Pharmazeutische Wirkstoffe in English, covering over 2,100 pharmaceutical compounds of interest to the chemical and pharmaceutical industry. Available in print and CD-ROM, Pharmaceutical Substances is designed to be a complete reference guide to every pharmaceutical compound of significance. It is essential as a first point of reference not only for specialists in drug chemistry but also for anyone involved in the synthesis or use of pharmaceuticals. All compounds are organized alphabetically according to INN standard, for added ease of use the book features three additional indexes: Intermediates Enzymes, Microorganisms, Plants and Animal Tissues Substance Classes The CD-ROM will allow field, full text and index searching. It includes a browse function to allow you to peruse the data alphabetically by INN. It will be updated annually with 50 to 100 new compounds added each year.
Transforming growth factor-beta production by synovial tissues from rheumatoid patients and streptococcal cell wall arthritic rats. Studies on secretion by synovial fibroblast-like cells and immunohistologic localization. (1989)

The growth of synovial fibroblast-like cells from patients with rheumatoid arthritis and rats with streptococcal cell wall (SCW)-induced arthritis in vitro under anchorage-independent conditions is inhibited by transforming growth factor-beta (TGF-beta). Because this growth factor is present in rheumatoid synovial fluids, we studied whether this cytokine might be secreted by cells in rheumatoid synovial tissue. We show that synovial tissues from patients with rheumatoid arthritis and osteoarthritis, and rats with SCW-induced arthritis, contain TGF-beta-1 mRNA. TGF-beta, predominantly type 1, was spontaneously secreted in vitro by synovial tissue explants and synovial fibroblast-like cells. In addition, TGF-beta could be detected immunohistochemically in cells throughout rheumatoid and SCW-induced arthritic rat synovial tissues. Finally, exogenous TGF-beta induced collagen and inhibited collagenase mRNA levels by cultured synoviocytes. These data support an autocrine role for TGF-beta in the regulation of synoviocytes in rheumatoid arthritis and, in light of its demonstrated effects on the immune system, suggest that TGF-beta might also have important paracrine effects on infiltrating inflammatory cells.
EDUCAÇÃO AMBIENTAL PARA EMPRESAS: CARACTERIZAÇÃO, DEFINIÇÃO E BOAS PRÁTICAS (2018)

Este artigo aborda a area de educacao ambiental, sua caracterizacao, definicao e boas praticas possuem como objetivo caracterizar algumas tecnicas que levam a empresa a atingir consideraveis resultados. Sera apresentado nas proximas paginas, todo conteudo teorico indispensavel para compreender este artigo, seus termos, definicoes e as tecnicas para implantacao das melhorias propostas e exemplos de boas praticas que podem ser utilizadas, portanto, este artigo revela as praticas fundamentais da educacao ambiental em empresas, com intuito de conscientizacao e mudanca de atitude, planejando e executando as atividades de acordo com um desenvolvimento ambiental, economicamente sustentavel, refletindo dentro e fora da empresa.
Update on the phase II trial and correlative studies of depsipeptide in patients with cutaneous T-cell lymphoma and relapsed peripheral T-cell lymphoma (2004)

3028 Background: Depsipeptide, FK228 (Fujisawa Pharmaceuticals), is the first of a new generation of histone deacetylase inhibitors to demonstrate consistent clinical activity in a specific tumor type. As a result, we are currently conducting a multi-institutional phase II trial in patients with CTCL, PTCL, or other mature T cell lymphomas. Methods: This trial is accruing patients into 4 separate arms based on histology and prior therapy. Depsipeptide is administered as a 4 hr infusion on days 1, 8, and 15 of a 28 d cycle. Results: Thirty-nine patients have been enrolled. In Arm 1, comprised of patients with CTCL who had not previously received more than 2 cytotoxic chemotherapy regimens, objective responses were observed in 7 of 14 patients, including 3 patients with complete response and 4 patients with partial response, for a response rate of 50%. Duration of response ranged from 6 to continuing over 34 months. Of the 17 patients with PTCL enrolled, 4 (24%) achieved a PR lasting 9 and 12 months in 2 patients, and ongoing over 4 months in 2 patients. Overall the drug is well tolerated, with observed toxicities including nausea, vomiting, fatigue, neutropenia, thrombocytopenia, and hypocalcemia. Non-specific ST-T wave changes are noted by ECG, without changes in cardiac function. Six patients, all with objective responses, have received depsipeptide for 12 to 34 months without evidence of cumulative toxicity. Molecular endpoints have been studied in patient normal and malignant cells. Increased acetylation of histone H3 has been observed in patient samples obtained 4 hr after initiation of the depsipeptide infusion. Inductions ranging from 2 to 24-fold were observed in 19 out of 24 paired samples obtained from individual patients, with 13 patients demonstrating a greater than 4-fold induction. Changes in gene expression have also been noted. Conclusions: These results suggest that histone deacetylase inhibitors such as depsipeptide are active in patients with T cell lymphoma. All 4 arms remain open to accrual at the NCI and extramural sites. Molecular effects can be assayed in patients treated with HDIs. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Fujisawa Pharmaceutical Company
Regional hemodynamic effects of calcitonin gene-related peptide (1989)

Cardiovascular responses to infusions of rat alpha-calcitonin gene-related peptide (CGRP; 0.06, 0.6, 6.0 nmol/h) or rat alpha-atrial natriuretic peptide (ANP; 3.7 nmol/h) were measured in conscious rats. During infusion of the low dose of CGRP, when mean arterial pressure (MAP) was little affected, there were reductions in common carotid, renal, mesenteric, and hindquarter vascular resistances (the magnitude of the responses in the same descending order). However, only flow in the common carotid vessels was increased above base line. After infusion, there was a hindquarter vasoconstriction. During infusion of the higher doses of CGRP, there were dose-related decreases in MAP and increases in heart rate associated with (hyperemic) hindquarter vasodilatations and mesenteric vasoconstrictions. The common carotid vasodilatation peaked with the intermediate dose of CGRP; the changes in renal vascular resistance were not dose related. After infusion of the high dose of CGRP there were persistent (at least 60 min) common carotid and hindquarter vasodilatations and mesenteric vasoconstriction, with a transient overshoot in renal vascular resistance. Infusions of CGRP and ANP matched for their effects on MAP had similar influences on mesenteric hemodynamics, but all other variables were affected differently.
REMOVAL OF PARTICULATE CONTAMINANTS USING ULTRASONICS AND MEGASONICS: A REVIEW (1995)

ABSTRACT Sonic cleaning is a commonly used technique for removal of particles from surfaces. The theory of particle removal using ultrasound is reviewed. Effects of cavitation and acoustic streaming are discussed. Experimental results using ultrasonics and megasonics (ultrasonic cleaning near 1 MHz) from the literature are reviewed. Emphasis is placed on removal of fine particles from silicon surfaces for semiconductor manufacturing applications.
New system stops paraffin buildup (1989)

Paraffin buildup in many producing oil wells can be a serious production constraint. Heat is considered a universal paraffin control technique. The authors present a system called paratrol. It provides a method for safely introducing electricity to the tubing string to convert the tubing string itself into a heating element. The entire well-head remains electrically grounded because of the way the electrical current is introduced. The temperature the electric current is introduced. The temperature produced in the heated tubing is typically at the temperature of the paraffin cloud point or above. The heating system discussed here was designed to provide an automatic, unattended, reliable paraffin control system for the production tubing of oil wells.
Three-Dimensional Optical Data Storage Using Photochromic Materials (2000)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThree-Dimensional Optical Data Storage Using Photochromic MaterialsSatoshi Kawata and Yoshimasa KawataView Author Information Department of Applied Physics, Osaka University, Suita, Osaka, 565-0871, Japan, and Department of Mechanical Engineering, Shizuoka University, Johoku, Hamamatsu, 432-8561, Japan Cite this: Chem. Rev. 2000, 100, 5, 1777–1788Publication Date (Web):April 25, 2000Publication History Received14 June 1999Published online25 April 2000Published inissue 1 May 2000https://pubs.acs.org/doi/10.1021/cr980073phttps://doi.org/10.1021/cr980073presearch-articleACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views9849Altmetric-Citations1285LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Circuits,Lasers,Layers,Light,Molecular structure Get e-Alerts
Calpain-dependent Proteolytic Cleavage of the p35 Cyclin-dependent Kinase 5 Activator to p25 (2000)

Cyclin-dependent kinase 5 (CDK5) is a unique CDK, the activity of which can be detected in postmitotic neurons. To date, CDK5 purified from mammalian brains has always been associated with a truncated form of the 35-kDa major brain specific activator (p35, also known as nck5a) of CDK5, known as p25. In this study, we report that p35 can be cleaved to p25 both in vitro and in vivo by calpain. In a rat brain extract, p35 was cleaved to p25 by incubation with Ca(2+). This cleavage was inhibited by a calpain inhibitor peptide derived from calpastatin and was ablated by separating the p35.CDK5 from calpain by centrifugation. The p35 recovered in the pellet after centrifugation could then be cleaved to p25 by purified calpain. Cleavage of p35 was also induced in primary cultured neurons by treatment with a Ca(2+) ionophore and Ca(2+) and inhibited by calpain inhibitor I. The cleavage changed the solubility of the CDK5 active complex from the particulate fraction to the soluble fraction but did not affect the histone H1 kinase activity. Increased cleavage was detected in cultured neurons undergoing cell death, suggesting a role of the cleavage in neuronal cell death.
Biochemistry of metallothionein (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiochemistry of metallothioneinJeremias H. R. Kaegi and Andreas SchaefferCite this: Biochemistry 1988, 27, 23, 8509–8515Publication Date (Print):November 15, 1988Publication History Published online1 May 2002Published inissue 15 November 1988https://pubs.acs.org/doi/10.1021/bi00423a001https://doi.org/10.1021/bi00423a001research-articleACS PublicationsRequest reuse permissionsArticle Views2210Altmetric-Citations919LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
THE ESSENTIAL OIL OFCANNABIS SATIVA (1975)

In previous reports the presence of cannabinoids in the distilled essential oil of Cannabis sativa L. was proved, besides the presence of mono– and sesquiterpene hydrocarbons.
Modulation Broadening of NMR and ESR Line Shapes (1963)

An analysis is made of the phase sensitive detection of magnetic resonances at various harmonics of the modulation frequency. The results are applicable to any experiment in which phase sensitive detection is employed. A mathematical treatment of modulation broadening is developed. Unlike the Taylor expansion method this is applicable in all practical cases. Signal amplitude and line shape of Lorentz and Gaussian resonances are calculated for the first three harmonics as functions of the modulation amplitude. Expressions which relate the Fourier coefficients of a modulation-broadened resonance line shape to those of the true integrated line shape are derived. The use of these expressions in correcting observed line shapes for modulation broadening is compared with that of another method involving the summation of a convergent series.
Systemic or mucosal administration of immunostimulatory DNA inhibits early and late phases of murine allergic conjunctivitis (2000)

Seasonal allergic conjunctivitis is one of the most common manifestations of allergic disease, affecting 15 % population in the United States annually. Short ragweed (RW) is a major cause of seasonal allergies. Immunostimulatory DNA sequences (ISS or CpG motifs) can inhibit an on-going Th2/allergic response and induce a de novo Th1 response. In this study, we investigated the ability of these ISS to modulate allergic responses in a RW-induced mouse model of seasonal allergic conjunctivitis. Systemic or mucosal administration of ISS oligonucleotide (ISS-ODN) after RW sensitization inhibited both the immediate hypersensitivity response and the late-phase cellular infiltration and induced a RW-specific Th1 response. ISS-ODN administration suppressed the rise of RW-specific IgE titers after repeated allergen challenge. Furthermore, ISS administration was more effective than dexamethasone in inhibiting the allergic response. Mechanistically, the ISS-induced immunomodulatory effects were abolished when mice were treated with anti-IL-12 neutralizing antibodies, suggesting a pivotal role for type 1 cytokines in the inhibition of both the immediate hypersensitivity and the late-phase cellular infiltration. Thus, ISS-ODN is a novel anti-inflammatory and immunomodulatory agent that significantly inhibits the allergic response and may provide an alternative to the current standard care of ocular allergy.
Metal−Organic Coordination Interactions in Fe−Terephthalic Acid Networks on Cu(100) (2008)

Metal-organic coordination interactions are prime candidates for the formation of self-assembled, nanometer-scale periodic networks with room-temperature structural stability. We present X-ray photoelectron spectroscopy measurements of such networks at the Cu(100) surface which provide clear evidence for genuine metal-organic coordination. This is evident as binding energy shifts in the O 1s and Fe 3p photoelectron peaks, corresponding to O and Fe atoms involved in the coordination. Our results provide the first clear evidence for charge-transfer coordination in metal-organic networks at surfaces and demonstrate a well-defined oxidation state for the coordinated Fe ions.