gebhart/patrepeval huggingface dataset:
Determining optimal dose of laser therapy for attachment and proliferation of human oral fibroblasts cultured on titanium implant material (2005)

The purpose of this study was to investigate the influence of single or multiple doses of low-level laser therapy (LLLT) on attachment and proliferation of human gingival fibroblasts in a standardized, reproducible in vitro model. Titanium discs were randomly allotted to one of three groups: group I served as a control, group II was exposed to a single laser dose of 3 J/cm2, and the three subgroups in group III were exposed to laser doses of 0.75, 1.5, and 3 J/cm2. To examine the possible thermal effects of laser exposure on the cell culture, the temperature in the Petri dish was measured for every dose used, before and during irradiation. For attachment assays, groups II and III were exposed to laser irradiation and then seeded onto titanium discs. In group III, the exposures were repeated after 3 and 6 h. Cells were cultured for 6 and 24 h and stained with Hoechst and Propidium. Attached cells were counted under a light microscope. To investigate the effect of LLLT on cell proliferation after 48 h, 72 h, and 7 days, cells were cultured on titanium discs for 24 h and then exposed to laser irradiation for 1 day and 3 consecutive days, respectively. Cell proliferation was determined by counting cells under the microscope and by a cell proliferation enzyme-linked immunosorbent assay system. No increase of temperature of the cell cultures occurred before or during laser exposure at any of the doses tested. Both single and multiple doses of LLLT significantly enhanced cellular attachment (p<0.05). The proliferation assays showed higher cell proliferation (p<0.05) in group III at doses of 1.5 and 3 J/cm2 after 72 h and 7 days, with agreement between staining and enzyme-linked immunosorbent assay. It is concluded that, in this cellular model, the attachment and proliferation of human gingival fibroblasts are enhanced by LLLT in a dose-dependent manner.
qPCR primer design revisited (2017)

Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative detection of amplification targets. Despite the framework provided by the MIQE guidelines and the accessibility of wide-ranging support from peer-reviewed publications, books and online sources as well as commercial companies, the design of many published assays continues to be less than optimal: primers often lack intended specificity, can form dimers, compete with template secondary structures at the primer binding sites or hybridise only within a narrow temperature range. We present an overview of the main steps in the primer design workflow, with data that illustrate some of the unexpected variability that often occurs when theory is translated into practice. We also strongly urge researchers to report as much information about their assays as possible in their publications.
Cloning of the shark Po promoter using a genomic walking technique based on the polymerase chain reaction (1990)

We have cloned the putative shark promoter of protein zero (Po) using a novel application of the ligation mediated single-sided polymerase chain reaction (PCR) method. This method uses linker ligation and subsequent amplifications with a linker primer and multiple specific primers to generate specificity. The method allowed us to amplify approximately 305 base pairs of shark genomic DNA sequence immediately upstream from the 5' end of our full-length Po cDNA. The Po promoter was shown to be directly linked to its first exon, contain a transcription initiation start site and sequences commonly found in eukaryotic promoters. This genomic walking technique will be useful for cloning promoters, insertion sites, and other sequences of interest without the need for constructing and screening genomic libraries.
Decision Support Systems for Improving the Quality of Medical Care (2008)

The successful delivery of medical care is a complex outcome that depends on multiple factors. Structural factors include the setting in which care takes place and related infrastructure that facilitate the care processes. The actual processes of medical care are defined by the completeness of the diagnosis and appropriateness and continuity of the resulting patient treatment. These factors are also highly dependent on valid, accurate, complete and reliable data collection and the use of that data to build models and systems to support the decision making processes involved. This presentation describes the computational, data communication, organizational and managerial components of a networked decision support system to improve medical care in a distributed healthcare system serving diverse patients. After data validation and cleaning, a statistical risk model is created that adjusts for the regional patient mix, loads and available structures of care. Using the model the system produces: (1) summary reports comparing patient outcomes over multiple periods, (2) dashboards to convey up to date status on utilization and outcomes and (3) a decision calculator for clinicians to calculate the riskiness of patient conditions - all intended to drive data driven decision making in the distributed healthcare system. Details of the data collection, data processing and system outputs and their use in medical decision making by various stakeholders -policy makers, regional hospital administrators, medical department chiefs and clinicians are illustrated and organizational/managerial best practices are presented. These practices coupled with the communication technologies have implications for distributed DSS practitioners and researchers.
Mannose-binding lectin polymorphisms are not associated with rheumatoid arthritis--confirmation in two large cohorts (2008)

MBL genotype groups are not associated with RA disease susceptibility or severity in this large study including a confirmation cohort. Compared with previous smaller studies these results add to more definite conclusions.
Redistribution of Cyclophilin A to Viral Factories during Vaccinia Virus Infection and Its Incorporation into Mature Particles (2003)

Cyclophilins are peptidyl-prolyl cis-trans isomerases involved in catalyzing conformational changes and accelerating the rate of protein folding and refolding in several cellular systems. In the present study, we analyzed the expression pattern and intracellular distribution of the cellular isomerase cyclophilin A (CypA) during vaccinia virus (VV) infection. An impressive increase in CypA stability was observed, leading to a practically unchanged accumulation of CypA during infection, although its synthesis was completely inhibited at late times. By confocal microscopy, we observed that CypA went through an intense reorganization in the cell cytoplasm and colocalized with the virosomes late in infection. CypA relocation to viral factories required the synthesis of viral postreplicative proteins, and treatment of infected cells with cyclosporine (CsA) prevented CypA relocation, clearly excluding the virosomes from CypA staining. Immunoelectron microscopy of VV-infected cells showed that CypA was incorporated into VV particles during morphogenesis. Biochemical and electron microscopic assays with purified virions confirmed that CypA was encapsidated within the virus particle and localized specifically in the core. This work suggests that CypA may develop an important role in VV replication.
Detailed analysis of sputum and systemic inflammation in asthma phenotypes: are paucigranulocytic asthmatics really non-inflammatory? (2016)

This study demonstrates that a significant eosinophilic inflammation is present across all categories of asthma, and that paucigranulocytic asthma may be seen as a low grade inflammatory disease.
Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stress (2007)

The effect of osmotic stress on the intracellular diffusion of proteins in Escherichia coli was studied, using a pulsed version of fluorescence recovery after photo-bleaching, pulsed-FRAP. This method employs sequences of laser pulses which only partly bleach the fluorophores in a cell. Because the cell size and geometry are taken into account, pulsed-FRAP enables to measure diffusion in very small cells of different shapes. We found that upon an osmotic upshock from 0.15 to 0.6 Osm, imposed by NaCl or sorbitol, the apparent intracellular diffusion (D) of mobile green fluorescent protein (GFP) decreased from 3.2 to 0.4 microm(2) s(-1), whereas the membrane permeable glycerol had no effect. Exposing E. coli cells to higher osmolalities (> 0.6 Osm) led to compartmentalization of the GFP into discrete pools, from where the GFP could not escape. Although free diffusion through the cell was hindered, the mobility of GFP in these pools was still relatively high (D approximately 0.4 microm(2) s(-1)). The presence of osmoprotectants restored the effect of osmotic stress on the protein mobility and apparent compartmentalization. Also, lowering the osmolality from 0.6 Osm back to 0.15 Osm restored the mobility of GFP. The implications of these findings in terms of heterogeneities and diffusive barriers inside the cell are discussed.
Vector fluid (2010)

We present a simple technique for creating fluid silhouettes described with vector graphics, which we call "Vector Fluid." In our system, a solid region in the fluid is represented as a closed contour and advected by fluid flow to form a curly and clear shape similar to marbling or sumi-nagashi (See Figure 1). The fundamental principle behind our method is that contours of solid regions should not collide. This means that if the initial shape of the region is a concave polygon, that shape should maintain its topology so that it can be rendered as a regular concave polygon, no matter how irregularly the contour is distorted by advection. In contrast to other techniques, our approach explicitly neglects topology changes to track surfaces in a trade off of computational cost and complexity. We also introduce an adaptive contour sampling technique to reduce this extra cost. We explore specific examples in 2D for art oriented usage and show applications and robustness of our method to exhibit organic fluid components. We also demonstrate how to port our entire algorithm onto a GPU to boost interactive performance for complex scenes.
Embryonic stem (ES) cells and embryonal carcinoma (EC) cells: opposite sides of the same coin (2005)

Embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and the malignant counterparts of embryonic stem (ES) cells derived from the inner cell mass of blastocyst-stage embryos, whether human or mouse. On prolonged culture in vitro, human ES cells acquire karyotypic changes that are also seen in human EC cells. They also ‘adapt’, proliferating faster and becoming easier to maintain with time in culture. Furthermore, when cells from such an ‘adapted’ culture were inoculated into a SCID (severe combined immunodeficient) mouse, we obtained a teratocarcinoma containing histologically recognizable stem cells, which grew out when the tumour was explanted into culture and exhibited properties of the starting ES cells. In these features, the ‘adapted’ ES cells resembled malignant EC cells. The results suggest that ES cells may develop in culture in ways that mimic changes occurring in EC cells during tumour progression.
Enhancement of the transverse conductance in DNA nucleotides (2008)

We theoretically study the electron transport properties of DNA nucleotides placed in the gap between two single-wall carbon nanotubes capped or terminated with H or N. We show that in the case of C-cap and H-termination the current at low electric bias is dominated by nonresonant tunneling, similarly to the cases of gold electrodes. In nitrogen-terminated nanotube electrodes, the nature of current is primarily quasiresonant tunneling and is increased by several orders of magnitude. We discuss the consequence of our result on the possibility of recognition at the level of single molecule.
Safety of Converting From Tetrabenazine to Deutetrabenazine for the Treatment of Chorea (2017)

In patients with chorea, overnight conversion to deutetrabenazine therapy provided a favorable safety profile and effectively maintained chorea control.
Grey System Theory Modeling for Nonlinear, Dynamic Machine Tool Thermal Error (2009)

To accommodate the nonlinear and dynamic nature of thermal elastic process, Grey System Theory (GST) is adopted. By using this theory, GM (2, 1) and GM (1, 4) models are constructed. Real cutting experiment on a turning machine is conducted to establish and validate the model performance in terms of generalization ability. The comparison indicates that GM (2, 1) and GM (1, 4) perform better than other static and dynamic models such as Back Propagation Neural Network (BP) and Auto-regression Moving Average (ARMA). In addition, each of the two proposed model has their own advantages and they can be applied in practice.
The Valuation of Option Features in Retirement Benefits (1995)

This article extends and applies a contingent claims valuation approach to option features in retirement fund benefits. The approach incorporates retirement, mortality, and other decrements and allows for differences between the benefit payments and standard option payoffs. A discrete lattice implementation commonly used for valuing financial options is found to be unsatisfactory for the valuation of these option features. Simulation is found to be more efficient. Simulation is then used to calculate benefit values for a range of ages for a simplified fund. The results of the valuations for a number of simulation scenarios demonstrate that a traditional deterministic actuarial valuation can understate the cost of these benefits by as much as 25 to 35 percent.
Oncogenes and onco-suppressor gene in adenocarcinoma of the oesophagus. (1992)

While the activation of the proto-oncogenes has been implicated in the development and progression of cancer of many tissues, the role of oncogenes in the development of oesophageal adenocarcinoma has not been defined. Fifteen patients who had undergone resection for oesophageal adenocarcinoma and 15 who had undergone oesophagectomy or biopsy for Barrett's oesophagus were studied. The latter patients also had adjacent normal gastric mucosa biopsied for comparison with the metaplastic oesophageal mucosa. The mucosal samples were snap frozen and subsequently stained with monoclonal antibodies to the following oncogene associated proteins; c-erbB2 (neu and CE-1) (external domain), c-erbB2 (NCL-CB11) (internal domain), c-src, c-ras, c-myc, c-fos, c-jun, and the onco-suppressor gene--p53. All tumours were well or moderately differentiated adenocarcinomas arising from the lower third of the oesophagus. Eleven specimens showed strong membraneous staining with both c-erbB2 (neu) and c-erbB2 (CBL-CB11). Seven specimens showed strong nuclear staining with p53 onco-suppressor gene. Three specimens were positive for c-ras and c-src, and two were positive for c-jun. In Barrett's epithelium, nine specimens were positive for c-erbB2 (neu and CB11), three were positive for c-src, two were positive for c-ras and c-jun, and one was positive for c-fos. Two of the gastric mucosal biopsy specimens expressed c-erbB2 weakly but no other oncogenes were found. The frequency of positive staining for c-erbB2 is very high, compared with the expression of these genes in other tumours. It is also concluded that errors in the onco-suppressor gene p53, and especially in the external and internal domains of c-erbB2, which is also often expressed in Barrett's mucosa, may be implicated in the development of adenocarcinoma of the oesophagus.
Replication of Dengue-2 Virus in Cultured Human Lymphoblastoid Cells and Subpopulations of Human Peripheral Leukocytes (1976)

We studied the susceptibility of four human lymphoblastoid cell lines (HCL) and of subpopulations of circulating peripheral human leukocytes to dengue-2 virus infection. HCL with B cell characteristics (Raji, Wil 2WT, 8866), B-type peripheral lymphocytes, and macrophages were productively infected by dengue-2 virus. In contrast, an HCL with T cell characteristics (MOLT-4), T type peripheral lymphocytes, and polymorphonuclear (PMN) cells did not become infected and replicate dengue-2 virus. PMN cells did not adsorb dengue-2 virus, suggesting lack of viral receptors. However, T-type cultured lymphoblasts and T-type peripheral lymphocytes adsorbed dengue-2 virus, suggesting that the block in viral replication involves some stage of infection occurring after adsorption. Permissiveness of B-type HCL to dengue-2 virus infection was dependent on the virus seed used but the virus titers obtained among the susceptible HCL varied. HCL infected persistently with dengue-2 virus have been established. Human peripheral lymphocytes inoculated after cultivation for 3 days in complete medium alone or complete medium supplemented with mitogens replicated dengue-2 virus. In contrast, unstimulated peripheral lymphocytes inoculated immediately after isolation adsorbed dengue-2 but did not support its replication. Mitogen-treated and untreated macrophages replicated dengue-2 virus equally well. The efficiency of dengue-2 virus replication by macrophages was higher than that of peripheral lymphocytes but lower than that of HCL.
Handbook of Raman Spectroscopy: From the Research Laboratory to the Process Line (2001)

Theory of Raman scattering evolution and revolution of Raman instrumentation - application of available technologies to spectroscopy and microscopy Raman spectroscopy and its adaptation to the industrial environment Raman microscopy - confocal and scanning near-field Raman imaging the quest for accuracy in Raman spectra chemometrics for Raman spectroscopy Raman spectra of gases Raman spectroscopy applied to crystals - phenomena and principles, concepts and conventions Raman scattering of glass Raman spectroscopic applications to gemmology Raman spectroscopy on II-IV-semiconductor nanostructures medical applications of Raman spectroscopy - in vivo Raman spectroscopy some pharmaceutical applications of Raman spectroscopy low-frequency Raman spectroscopy and biomolecular dynamics - a comparison between different low-frequency experimental techniques collectivity of vibrational modes Raman spectroscopic studies of ion-ion interactions in aqueous and non-aqueous electrolyte solutions environmental applications of Raman spectroscopy to aqueous solutions Raman and surface enhanced resonance Raman scattering - applications in forensic science application of Raman spectroscopy to organic fibres and films applications of IR and Raman spectra of quasi-elemental carbon process Raman spectroscopy the use of Raman spectroscopy to monitor the quality of carbon overcoats in the disk drive industry Raman spectroscopy in the undergraduate teaching laboratory Raman spectroscopy in the characterization of archaeological materials.
Alternative process for thin layer etching: Application to nitride spacer etching stopping on silicon germanium (2014)

Silicon nitride spacer etching realization is considered today as one of the most challenging of the etch process for the new devices realization. For this step, the atomic etch precision to stop on silicon or silicon germanium with a perfect anisotropy (no foot formation) is required. The situation is that none of the current plasma technologies can meet all these requirements. To overcome these issues and meet the highly complex requirements imposed by device fabrication processes, we recently proposed an alternative etching process to the current plasma etch chemistries. This process is based on thin film modification by light ions implantation followed by a selective removal of the modified layer with respect to the non-modified material. In this Letter, we demonstrate the benefit of this alternative etch method in term of film damage control (silicon germanium recess obtained is less than 6 A), anisotropy (no foot formation), and its compatibility with other integration steps like epitaxial. The etch mechanisms of this approach are also addressed.
The Cardiovascular, Pulmonary, and Ocular Hypotensive Effects of 0.2% Brimonidine (1995)

The cardiopulmonary effects of 0.2% brimonidine were limited to a slight reduction in systolic blood pressure during recovery from exercise and at 4 hours after instillation. The ocular hypotensive effect of brimonidine was comparable to that of timolol and greater than that of betaxolol suspension in this patient population.
Upregulation of the Wnt/?-catenin Pathway Induced by Transforming Growth Factor-? in Hypertrophic Scars and Keloids (2006)

Hypertrophic scars and keloids represent a dysregulated response to cutaneous wounds, which results in an excessive deposition of collagen. Transforming growth factor-beta (TGF-beta) is the key regulator in the pathogenesis of fibrosis. Accumulating evidence suggests that Wnt signalling and its effector beta-catenin also play an important role in wound healing. The role of Wnt/beta-catenin signalling in TGF-beta induced collagen deposition in hypertrophic scars and keloids was studied. Transcriptional assays and Western blotting was performed using fibroblast cell lines established from normal skin and hypertrophic scar tissue. Immunohistochemical studies were performed using scar tissues. We provide evidence that TGF-beta induces activation of beta-catenin mediated transcription in human dermal fibroblasts via the Smad3 and p38 MAPK pathways. Immunohistochemical studies demonstrated that beta-catenin protein levels are elevated in hypertrophic scar and keloid tissues. This finding may be relevant to the pathogenesis of hypertrophic scars and keloids.
Lymphopenia in treatment-naive relapsing multiple sclerosis (2016)

Lymphopenia accompanies some autoimmune diseases.1 Several studies,2,3 but not others,4,5 have suggested that lymphopenia occurs in treatment-naive multiple sclerosis (MS), so the issue remains unresolved. This is important since lymphopenia may identify an immunologically distinct subset of MS. Also, lymphopenia may emerge as a risk factor for serious viral infections of the brain during dimethyl fumarate treatment.2 We therefore embarked on a retrospective controlled study of pretreatment lymphopenia in relapsing MS.
E5531, AN ENDOTOXIN ANTAGONIST, BLOCKS THE HYPERDYNAMIC AND DEPRESSANT CARDIOVASCULAR EFFECTS OF ENDOTOXIN IN HEALTHY SUBJECTS (1995)

Bunnell, Eugene1; Neumann, Alex1; Lynn, Melvyn2; Friedhoff, Lawrence T.2; Rogers, Sharon L.2; Habet, Kalim1; Parrillo, Joseph E.1 Author Information