gebhart/patrepeval huggingface dataset:
Involvement of Gap Junctions in the Manifestation and Control of the Duration of Seizures in Rats In Vivo (2003)

After repeated seizures, the expression levels of Cx32, Cx43, and Cx36 mRNAs at the epileptic foci were increased significantly. Blockade of the gap junctions with carbenoxolone shortened the duration of seizures and decreased the amplitude of the seizure discharges, whereas their opening with trimethylamine lengthened the duration and increased the amplitude. Secondary epileptogenesis was facilitated when the gap junctions were opened. CONCLUSIONS; Our findings support the idea that, in epileptic foci, the gap junctions are involved in the expression of rhythmic ictal discharges and in the control of the duration and propagation of the individual seizures in vivo.
Preceramic polymer routes to silicon carbide (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreceramic polymer routes to silicon carbideRichard M. Laine and Florence BabonneauCite this: Chem. Mater. 1993, 5, 3, 260–279Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/cm00027a007https://doi.org/10.1021/cm00027a007research-articleACS PublicationsRequest reuse permissionsArticle Views2588Altmetric-Citations276LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Planning procedures - a practical case: The Patea hydro electric scheme (1987)

The paper outlines the practical implementation of statutory planning requirements as undertaken for the Patea Hydro Electric Power Scheme. In particular it focuses on the order in which the approvals were sought and emphasises the need for public involvement from an early stage of planning. It also deals briefly with the follow-up implementation of the recommendations which emanated from the audits or hearings and the operation of the scheme to comply with the rights granted.
Expression and Localization of GEN1 in Mouse Mammary Epithelial Cells (2014)

GEN1, a Holliday junction resolvase, is involved in homologous repair of DNA double strand break and in maintaining centrosome integrity. Although GEN1 mutants have been reported in breast cancer patients and cell lines, little is currently known about the functions of GEN1 in the development and oncogenic transformation of mammary gland. In the present study, we demonstrate that GEN1 expression is correlated with mammary epithelial cell proliferation, differentiation in various physiological stages as well as casein. By immunofluorescence analysis, the centrosomal association of GEN1 is confirmed in mammary epithelial cells. Additionally, GEN1 is likely involved in DNA damage response of breast cancer cell lines. These results suggest that GEN1 may play an important role in the development of mammary gland; its response upon DNA damage indicates that GEN1 gene alteration may contribute to breast cancer formation.
Enhanced epitopic response to a synthetic human malarial peptide by preimmunization with tetanus toxoid carrier (1987)

Successful human vaccination by synthetic malarial sporozoite peptides may depend on the choice of an appropriate carrier. Tetanus toxoid (TT) has been proposed because of its safe and widespread use in humans. Paradoxically, however, prior exposure to this toxoid vaccine could produce specific epitopic suppression against synthetic malarial peptides conjugated to this same protein as carrier. Indeed, we have previously reported that such a phenomenon can occur in the case of a synthetic vaccine made with a streptococcal peptide conjugated to TT. Our present study shows that similar results can be observed in mice preimmunized with TT 1 month before the administration of a conjugate containing TT and a Plasmodium knowlesi peptide. Analysis of the isotypic pattern of the antipeptide response showed that the immunoglobulin G1 (IgG1) subclass and especially the IgG2a and IgG2b subclasses were suppressed. In contrast, when a sporozoite peptide from Plasmodium falciparum was coupled to TT, the total antipeptide antibodies and particularly the IgG1 subclass were enhanced by preimmunization by TT. This increase of antipeptide antibodies was correlated with a greater ability of the sera to neutralize sporozoite infectivity. These results indicate that prior exposure to TT does not systematically impair the antibody response against a peptide administered as a peptide-TT conjugate.
Measurement and calculation of the two-dimensional backscattering Mueller matrix of a turbid medium (1998)

We present both experimental and Monte Carlo-based simulation results for the diffusely backscattered intensity patterns that arise from illumination of a turbid medium with a polarized laser beam. A numerical method that allows the calculation of all 16 elements of the two-dimensional Muller matrix is used; moreover, it is shown that only seven matrix elements are independent. To validate our method, we compared our simulations with experimental measurements, using a turbid medium consisting of 2.02-microm -diameter polystyrene spheres suspended in deionized water. By varying the incident polarization and the analyzer optics for the experimental measurements, we obtained the diffuse backscattering Mueller matrix elements. The experimental and the numerical results are in good agreement.
Synthesis and immunological properties of Vi and di-O-acetyl pectin protein conjugates with adipic acid dihydrazide as the linker (1997)

The Vi capsular polysaccharide of Salmonella typhi, a licensed vaccine for typhoid fever in individuals > or = 5 years old, induces low and short-lived antibodies in children, and reinjection does not elicit booster responses at any age. Its immunogenicity was improved by binding Vi to proteins by using N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) as a linker. Similar findings were observed with the structurally related, di-O-acetyl derivative of pectin [poly-alpha(1-->4)-D-GalpA] designated OAcP. Protein conjugates of Vi and OAcP were synthesized by carbodiimide-mediated synthesis with adipic acid dihydrazide (ADH) as the linker. Hydrazide groups were introduced into proteins (bovine serum albumin or recombinant Pseudomonas aeruginosa exoprotein A) by treatment with ADH and 1-ethyl-3(3-dimethylaminopropyl carbodiimide (EDC). The resultant adipic acid hydrazide derivatives (AH-proteins), containing 2.3 to 3.4% AH, had antigenic and physicochemical properties similar to those of the native proteins. The AH-proteins were bound to Vi and OAcP by treatment with EDC. The immunogenicity of Vi or OAcP, alone or as protein conjugates, was evaluated in young outbred mice and guinea pigs by subcutaneous injection of 2.5 and 5.0 microg, respectively, of polysaccharide, and antibodies were measured by enzyme-linked immunosorbent assay. All conjugates were significantly more immunogenic than Vi or OAcP alone and induced booster responses with 5- to 25-fold increases of antibodies. Vi conjugates were significantly more immunogenic than their OAcP analogs. A carboxymethyl derivative of yeast beta-glucan enhanced the anti-Vi response elicited by an OAcP conjugate but had no effect on the immunogenicity of Vi or of OAcP alone. Vi and OAcP conjugates synthesized by this scheme will be evaluated clinically.
Production and Characterization of a Novel Monoclonal Antibody Against Human Sortilin (2015)

Sortilin, as a member of Vps10p-domain sorting receptor family, is overexpressed in a number of malignancies, including ovarian carcinoma. Antibodies against sortilin may contribute to further clarification of sortilin functional activities in signal transduction, intracellular sorting of proteins, and endocytosis. The aim of this study was to produce a monoclonal antibody against a synthetic peptide derived from extracellular N-terminal region of sortilin to be used as a tool for investigating sortilin characteristics in ovarian carcinoma. A synthetic peptide derived from the last 50 amino acids of extracellular domain of sortilin protein was selected and conjugated to keyhole limpet hemocyanin and used to immunize mice. The anti-sortilin monoclonal antibody (MAb), clone 2D8, was purified from supernatant of final hybridoma clone using peptide-affinity chromatography column. Reactivity of antibody with the immunizing peptide was assessed in ELISA. Furthermore, flow cytometry and Western blot analyses were used to investigate the reactivity of antibody with its target in a panel of ovarian carcinoma cell lines or tissues. MAb 2D8 was able to recognize the coated immunizing peptide in ELISA and detect its protein target, sortilin, in flow cytometry and Western blot analyses. The achieved data suggest that the developed monoclonal antibody may be applicable as a research tool for detection of sortilin protein in Western blot as well as flow cytometry tests.
Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains. (1988)

Previous work (6-10) has shown that allelic exclusion of Ig gene expression is controlled by functionally rearranged mu and kappa genes. This report deals with the comparison of membrane mu (micron) and secreted mu (microsecond) in promoting such feedback inhibition. Splenic B cell hybridomas were analyzed from transgenic mice harboring a rearranged kappa gene alone or in combination with either an intact rearranged mu gene or a truncated version of the mu gene. The intact mu gene is capable of producing both membrane and secreted forms of the protein, while the truncated version can only encode the secreted form. The role of the microsecond was also tested in pre-B cell lines. Analysis of the extent of endogenous Ig gene rearrangement revealed that (a) the production of micron together with kappa can terminate Ig gene rearrangement; (b) microsecond with kappa does not have this feedback effect; (c) microsecond may interfere with the effect of micron and kappa; and (d) the feedback shown here probably represents a complete shutoff of the specific recombinase by micron + kappa; the data do not address the question of mu alone affecting the accessibility of H genes for rearrangement.
Interleukin-1 Costimulatory Activity on the Interleukin-2 Promoter Via AP-1 (1989)

Interleukin-1 (IL-1) is a major regulator of inflammation and immunity. IL-1 induces T lymphocyte growth by acting as a second signal (together with antigen) in enhancing the production of interleukin-2 (IL-2). An IL-1-responsive element in the promoter region of the human IL-2 gene was similar to the binding site for the transcription factor AP-1. IL-1 enhanced expression of c-jun messenger RNA, whereas the antigenic signal enhanced messenger RNA expression of c-fos. Thus, the two components of the AP-1 factor are independently regulated and the AP-1 factor may serve as a nuclear mediator for the many actions of IL-1 on cells.
A Metabolite of Riboflavin Binds to the 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) Receptor (1985)

A cytosolic receptor to which TCDD binds with high affinity has been reported in a number of rodent tissues, but the endogenous/natural ligand is not known. However, lumichrome, a metabolite of riboflavin, competed with 3H-TCDD for binding to the cytosolic receptor. Neither riboflavin nor riboflavin-5-phosphate competed for binding to the TCDD receptor in rat liver cytosol. Lumichrome is the first known endogenous chemical in the rat to exhibit affinity for the TCDD receptor.
Dolastatins 23: stereospecific synthesis of dolaisoleuine (1996)

The remarkable antineoplastic peptide dolastatin 10, isolated from the opisthobranchia mollusc Dolabella auricularia, is currently in clinical development and further improvements in its total synthesis have been undertaken. Major effort has been directed at devising more stereoselective routes to the dolastatin 10 amino acid units dolaisoleuine 2 and dolaproine 3, each bearing three chiral centres. We report herein highly stereoselective routes to both natural (3R,4S,5S)-dolaisoleuine 2 and its 3S,4S,5S-isomer 14 (Z replaces H) using an asymmetric aldol methodology. Key reaction steps are condensation of chiral α-(methylsulfanyl)acetyloxazolidinone 4d with (S)-N-Z-N-Me-isoleucinal 6 using dibutylboron triflate followed by reductive desulfurization, O-methylation and cleavage of the oxazolidinone auxiliary to complete a simple route to N-benzyloxycarbonyldolaisoleuine 10. By substituting chiral oxazolidinone 5d for 4d the 3S-isomer of N-benzyloxycarbonyldolaisoleuine 14 was selectively obtained.
Dual focal adhesion kinase/Pyk2 inhibitor has positive effects on bone tumors (2008)

The oral administration of PF-562,271 at a dose of 5 mg/kg suppressed the growth and local spread of intratibial tumors and restored tumor-induced bone loss. The unique ability of PF-562,271 to both curb tumor growth and safely increase bone formation may be an effective therapy for many cancer patients with bone metastases and cancer-associated osteoporosis.
Refinement of insulator-based dielectrophoresis (2017)

The ability to separate analytes with increasingly similar properties drives the field of separation science. One way to achieve such separations is using trapping and streaming dielectrophoresis (DEP), which directly exploits the subtle differences in the electrophysical properties of analytes. The non-uniform fields necessary for DEP can be formed using various insulator shapes in microchannels. Current insulator shapes include triangles, diamonds, circles, and rectangles. However, all of these insulators pose problems for trapping, streaming, and sorting (deflection) as the induced fields/gradients are not behaviorally consistent across the lateral dimension. This leads to analytes experiencing different forces depending on their pathline in the microchannel and result in low resolution separations. Based on an iterative process that explored approximately 40 different insulator shapes, a design was chosen that indicated improved particle streamlines, better trapping efficiency, and consistent electrical environments across the lateral dimension. The design was assessed by simulations where the electric field, gradient of the electric field squared, and the ratio of the two were plotted. The improved design includes a unique new multi-length scale element. The multi-length scale structure streamlines the analyte(s) and improves homogeneity in the lateral dimension, while still achieving high gradients necessary for analyte separation using DEP. The design is calculated to keep analytes on the centerline which should improve resolution, and eliminate extraneous trapping zones. Behaviors consistent with the features of the simulations were observed in proof of principle experiments using representative test probes.
A 10/sup 13/ A/s high energy density micro discharge radiation source (2003)

The creation of a high brightness high energy density discharge based radiation source relies on a rapid delivery of electrical energy to a self contained plasma. Different types of pinch discharges have been studied in the past which produce localized regions of ultra-high energy density plasmas in the form of hot spots and micro pinches. These plasma features are not routinely reproducible in space and time. In this paper, the authors present a novel scheme for the production of a high energy density plasma based on a high current microcapillary discharge with effective source dimension of below 100 /spl mu/m in diameter and in length. The discharge is created directly in a structure in the energy storage dielectric, resulting in a discharge inductance of less than 1 nH. In this way, a peak current of 10 kA with a rise time of 10/sup 13/A-s/sup -1/ at the micro capillary discharge is achieved with an energy storage of 0.2 J on the capacitor. Experimental investigation on the plasma parameters and the influence of different capillary materials are presented. Results show that a kT=100 eV and n/sub e/=10/sup -19/ cm/sup -3/ plasma is formed emitting a 5 ns radiation pulse down to the soft X-ray region and fast ions of 8-10 keV due to free expansion.
Renal (pro)renin receptor contributes to development of diabetic kidney disease through transforming growth factor‐β1 – connective tissue growth factor signalling cascade (2011)

1. Transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) are expressed in renal glomeruli, and contribute to the development of diabetic nephropathy. Recently, we showed that (pro)renin receptor (PRR) is upregulated in the kidneys of the streptozocin (STZ)-induced diabetes rat model. We hypothesized that in the presence of hyperglycaemia, increased renal PRR expression contributes to enhanced TGF-β1-CTGF signalling activity, leading to the development of diabetic kidney disease. 2. In vivo and in vitro studies were carried out in Sprague-Dawley rats and rat mesangial cells (RMC). PRR blockade was achieved in vivo by treating STZ induced diabetes rats with the handle region peptide (HRP) of prorenin and in vitro by HRP or PRR siRNA in RMC. Angiotensin AT1 receptor blockade was achieved by valsartan treatment. 3. Results showed that expression of PRR, TGF-β1 and CTGF were upregulated in diabetic kidneys and RMC exposed to high glucose. Glucose exposure also induced PRR phosphorylation, a process that was inhibited by HRP, valsartan or PRR siRNA. HRP and valsartan significantly attenuated renal TGF-β1 and CTGF expression in diabetic animals and high glucose treated RMC. Similar results were observed in high glucose exposed RMC in response to PRR siRNA. TGF-β receptor blockade decreased CTGF expression in RMC. Combined administration of valsartan and PRR siRNA showed further reduction of TGF-β1 and CTGF expression in RMC. 4. In conclusion, PRR contributes to kidney disease in diabetes through an enhanced TGF-β1-CTGF signalling cascade.
Regulation of phosphate influx in barley roots: Effects of phosphate deprivation and reduction of influx with provision of orthophosphate (1982)

Barley plants were grown in nutrient solutions, which were maintained at either 0 (‐P) or 15 μ M orthophosphate (+P). After 11 days phosphate influx into the intact roots of the ‐P plants began to increase by comparison with +P plants. During this period differences became apparent between the treatments in absolute growth rates, as well as in the root:shoot ratios. Phosphate influx in the ‐P plants continued to increase as a function of time, to a maximum value of 2.4 μmol (g fresh wt) ‐1 h ‐1 at 16 days after germination. This rate was 6 times higher than influx values for +P plants of the same age. During the period of enhanced uptake phosphate was strongly correlated (r 2 = 0.77) with root organic phosphate concentration. – The enhancement of inorganic phosphate influx into intact roots of ‐P plants was rapidly reduced by the provision of 15 μ M orthophosphate. Typically, within 4 h of exposure to this concentration of phosphate, influx values fell from 1.80 ± 0.20 to 0.75 ± 0.03 μmol (g fresh wt) ‐1 h ‐1 , while inorganic phosphate concentrations of the roots increased from 0.12 to 1.15 μmol (g fresh wt) ‐1 during the same period. Hill plots of the influx data obtained during this period, treating root inorganic phosphate as an inhibitor of influx, gave Hill coefficients close to 2. The rapidity of the reduction of influx associated with increased root inorganic phosphate together with the Hill plot data provide evidence for an allosteric inhibition of influx by internal inorganic phosphate.
Characterization of the Pheromone Response of theEnterococcus faecalisConjugative Plasmid pCF10: Complete Sequence and Comparative Analysis of the Transcriptional and Phenotypic Responses of pCF10-Containing Cells to Pheromone Induction (2005)

The sex pheromone plasmids in Enterococcus faecalis are one of the most efficient conjugative plasmid transfer systems known in bacteria. Plasmid transfer rates can reach or exceed 10(-1) transconjugants per donor in vivo and under laboratory conditions. We report the completion of the DNA sequence of plasmid pCF10 and the analysis of the transcription profile of plasmid genes, relative to conjugative transfer ability following pheromone induction. These experiments employed a mini-microarray containing all 57 open reading frames of pCF10 and a set of selected chromosomal genes. A clear peak of transcription activity was observed 30 to 60 min after pheromone addition, with transcription subsiding 2 h after pheromone induction. The transcript activity correlated with the ability of donor cells to transfer pCF10 to recipient cells. Remarkably, aggregation substance (Asc10, encoded by the prgB gene) was present on the cell surface for a long period of time after pheromone-induced transcription of prgB and plasmid transfer ability had ceased. This observation could have relevance for the virulence of E. faecalis.
Complete completion using types and weights (2013)

Developing modern software typically involves composing functionality from existing libraries. This task is difficult because libraries may expose many methods to the developer. To help developers in such scenarios, we present a technique that synthesizes and suggests valid expressions of a given type at a given program point. As the basis of our technique we use type inhabitation for lambda calculus terms in long normal form. We introduce a succinct representation for type judgements that merges types into equivalence classes to reduce the search space, then reconstructs any desired number of solutions on demand. Furthermore, we introduce a method to rank solutions based on weights derived from a corpus of code. We implemented the algorithm and deployed it as a plugin for the Eclipse IDE for Scala. We show that the techniques we incorporated greatly increase the effectiveness of the approach. Our evaluation benchmarks are code examples from programming practice; we make them available for future comparisons.