gebhart/patrepeval huggingface dataset:
Development and Characterization of a Staphylococcus aureus Nasal Colonization Model in Mice (1999)

ABSTRACT Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital environment. Attenuation of carriage has proven effective in reducing the prevalence of infection in some high-risk groups. To study staphylococcal factors that influence nasal colonization, a mouse model of S. aureus nasal colonization was developed. Mice were inoculated intranasally with S. aureus Reynolds, and nasal carriage was evaluated by quantitating cultures of the nasal tissues from mice sacrificed at various time points after inoculation. The majority of mice inoculated with 10 8 CFU of S. aureus maintained nasal carriage for at least 20 days. Nasal colonization rates were similar for inbred (BALB/c and C57BL/6) and outbred (ICR) mice. Colonization was not affected by mouse passage of strain Reynolds. Lower inoculum doses (&lt;10 7 CFU) resulted in reduced colonization after 7 days. However, mice given streptomycin in their drinking water developed long-term carriage of S. aureus , and they were colonized with inocula as low as 10 5 CFU. Nasal colonization was also established with two other S. aureus strains (one strain each of human and murine origins). S. aureus recovered from the nares of experimentally colonized mice expressed high levels of capsule, and the ability of a capsule-defective mutant to persist in the nares was reduced in comparison to that of the parent strain. This nasal colonization model should prove useful for studies of factors that mediate S. aureus colonization and for assessment of targets for antimicrobial intervention or vaccine development.
Risks of Repeated Immunization (1975)

Comments and Corrections1 April 1975Risks of Repeated ImmunizationL. FRED AYVAZIAN, M.D.L. FRED AYVAZIAN, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-82-4-589_1 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptTo the editor: In their paper "Repeated Immunization: Possible Adverse Effects," White, Adler, and McGann (1) report on less than 10% of 700 male employees at Fort Derrick who since 1956 had received multiple immunizations; despite some interesting minor laboratory abnormalities in this group, the authors conclude that chronic stimulation of the immunoglobulin-stimulating system in the human seems to cause no adverse effects, unlike amyloid disease, myeloma, and hypersensitivity reported in laboratory animals. As in a similar study referred to, that is, the soldiers receiving influenza vaccine, the subjects studied were always or almost always men well above adolescent age....References1. WHITEADLERMCGANN CWV: Repeated immunization: possible adverse effects. Ann Intern Med 81:594-600, 1974 LinkGoogle Scholar2. AYVAZIANBADGER LT: Disseminated lupus erythematosus occurring among student nurses. N Engl J Med 239:565-570, 1948 CrossrefMedlineGoogle Scholar1. PEELERCLUFFTREVOR RLR: Hyperimmunization of man. Bull Johns Hopkins Hosp (Baltimore) 103:183-198, 1958 MedlineGoogle Scholar2. PEELERKADULLCLUFF RPL: Intensive immunization of man. Evaluation of possible adverse consequences. Ann Intern Med 63:44-57, 1965 LinkGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Pulmonary Disease Section Veterans Administration Hospital East Orange, New Jersey 07019 PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byInfluenza Immunization and Lupus ErythematosusL. FRED AYVAZIAN, M.D.Influenza Vaccination of Patients with Systemic Lupus ErythematosusSUSAN C. RISTOW, M.D., R. GORDON DOUGLAS Jr., M.D., F.A.C.P., JOHN J. CONDEMI, M.D., F.A.C.P. 1 April 1975Volume 82, Issue 4Page: 589-589KeywordsAdolescentsHypersensitivityMyelomaResearch laboratoriesRisk managementVaccines Issue Published: 1 April 1975 PDF downloadLoading ...
Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53. (1985)

Three clones for the human tumor antigen p53 were isolated from a cDNA library prepared from A431 cells. One of these clones, pR4-2, contains the entire coding region for human p53. This clone directs the synthesis of a polypeptide with the correct molecular weight and immunological epitopes of an authentic p53 molecule in an in vitro transcription-translation reaction. Although the pR4-2 clone contains the coding region for p53, it is not a full-length copy of the human p53 mRNA. Northern analysis showed that the p53 mRNA is approximately 2,500 nucleotides long, whereas the pR4-2 insert is only 1,760 base pairs in length. Analysis of the DNA sequence of this clone suggests that the human p53 polypeptide has 393 amino acids. We compared the predicted amino acid sequence of the pR4-2 clone with similar clones for the mouse p53 and found long regions of amino acid homology between these two molecules.
Covalent decoration of multi-walled carbon nanotubes with silica nanoparticles (2004)

We describe a novel tunable approach for the synthesis of carbon nanotube-silica nanobead composites. The control of nanotube morphology and bead size coupled with the versatility of silica chemistry makes these structures an excellent platform for the development of biosensors, or for optical, magnetic and catalytic applications.
The Use of Negative-Pressure Wound Therapy (NPWT) in the Temporary Treatment of Soft-Tissue Injuries Associated With High-Energy Open Tibial Shaft Fractures (2007)

Infection and nonunion rates with the use of NPWT for temporary coverage of wounds associated with grade/type III open tibial shaft fractures are similar to those of historical controls, but this technique may be beneficial in decreasing the need for free tissue transfer or rotational muscle flap coverage.
Perturbations in 4D marine seismic (2001)

A new concept for acquiring calibrated towed streamer seismic data is introduced through a new acquisition and processing system. The specification of the new system has been defined by rigorous analysis of the factors that limit the sensitivity of seismic data in 4D studies and imaging. New sensor and streamer technology, new source technology and advances in positioning techniques and data processing have addressed these limitations.Sensitivity analysis revealed that the most significant perturbations to the seismic signal are swell noise and sensor sensitivity variations. Conventional analog groups of hydrophones are designed to suppress swell noise however a new technique for data-adaptive coherent noise attenuation delivers even greater noise suppression for densely spatially sampled single-sensor data. Although modern source controllers provide accurate airgun firing control the signature of an airgun array may vary from shot to shot. This can be due to factors such as changes in the array geometry, air pressure variations, depth variations and wave action. A method for estimating the far-field signature of a source array is the Notional Source Method (proprietary to Schlumberger) which has been steadily refined since its first disclosure. A recent development compensates for variation in source array geometry by monitoring the position and azimuth of each subarray using GPS receivers mounted on the floats.New calibrated positioning and streamer control systems are part of the new acquisition system. Active vertical and lateral streamer control is achieved using steerable birds and positioning uncertainty is reduced through an in-built fully braced acoustic ranging system. Calibrated marine seismic data are achieved through quantifying the source output, the sensor responses and positioning uncertainty. The consequential improvements in seismic fidelity result in better imaging and more reliable 4D analysis.
Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral Agent (2020)

Remdesivir (RDV, GS-5734), the first FDA-approved antiviral for the treatment of COVID-19, is a single diastereomer monophosphoramidate prodrug of an adenosine analogue. It is intracellularly metabolized into the active triphosphate form, which in turn acts as a potent and selective inhibitor of multiple viral RNA polymerases. RDV has broad-spectrum activity against members of the coronavirus family, such as SARS-CoV-2, SARS-CoV, and MERS-CoV, as well as filoviruses and paramyxoviruses. To assess the potential for off-target toxicity, RDV was evaluated in a set of cellular and biochemical assays. Cytotoxicity was evaluated in a set of relevant human cell lines and primary cells. In addition, RDV was evaluated for mitochondrial toxicity under aerobic and anaerobic metabolic conditions, and for the effects on mitochondrial DNA content, mitochondrial protein synthesis, cellular respiration, and induction of reactive oxygen species. Last, the active 5'-triphosphate metabolite of RDV, GS-443902, was evaluated for potential interaction with human DNA and RNA polymerases. Among all of the human cells tested under 5 to 14 days of continuous exposure, the 50% cytotoxic concentration (CC<sub>50</sub>) values of RDV ranged from 1.7 to >20 μM, resulting in selectivity indices (SI, CC<sub>50</sub>/EC<sub>50</sub>) from >170 to 20,000, with respect to RDV anti-SARS-CoV-2 activity (50% effective concentration [EC<sub>50</sub>] of 9.9 nM in human airway epithelial cells). Overall, the cellular and biochemical assays demonstrated a low potential for RDV to elicit off-target toxicity, including mitochondria-specific toxicity, consistent with the reported clinical safety profile.
Phosphine-Catalyzed Asymmetric Synthesis of β-Lactones from Arylketoketenes and Aromatic Aldehydes (2010)

In this paper, the development of a chiral phosphine-catalyzed formal [2 + 2] cycloaddition of aldehydes and ketoketenes that provides access to a variety of highly substituted beta-lactones (14 examples) is reported. The BINAPHANE catalytic system displays excellent enantioselectivity (seven examples with ee >or=90%) and high diastereoselectivity favoring formation of the trans-diastereomer (nine examples with dr >or=90:10).
Expression, purification and characterization of amorpha-4,11-diene synthase from Artemisia annua L (2005)

Expression, purification and characterization of amorpha-4,11-diene synthase from Artemisia annua L
LAT2, a New Basolateral 4F2hc/CD98-associated Amino Acid Transporter of Kidney and Intestine (1999)

Glycoprotein-associated amino acid transporters (gpaAT) are permease-related proteins that require heterodimerization to express their function. So far, four vertebrate gpaATs have been shown to associate with 4F2hc/CD98 for functional expression, whereas one gpaAT specifically associates with rBAT. In this study, we characterized a novel gpaAT, LAT2, for which mouse and human cDNAs were identified by expressed sequence tag data base searches. The encoded ortholog proteins are 531 and 535 amino acids long and 92% identical. They share 52 and 48% residues with the gpaATs LAT1 and y(+)LAT1, respectively. When mouse LAT2 and human 4F2hc cRNAs were co-injected into Xenopus oocytes, disulfide-linked heterodimers were formed, and an L-type amino acid uptake was induced, which differed slightly from that produced by LAT1-4F2hc: the apparent affinity for L-phenylalanine was higher, and L-alanine was transported at physiological concentrations. In the presence of an external amino acid substrate, LAT2-4F2hc also mediated amino acid efflux. LAT2 mRNA is expressed mainly in kidney and intestine, whereas LAT1 mRNA is expressed widely. Immunofluorescence experiments showed colocalization of 4F2hc and LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. In conclusion, LAT2 forms with LAT1 a subfamily of L-type gpaATs. We propose that LAT1 is involved in cellular amino acid uptake, whereas LAT2 plays a role in epithelial amino acid (re)absorption.
FLAME-1, a Novel FADD-like Anti-apoptotic Molecule That Regulates Fas/TNFR1-induced Apoptosis (1997)

We identified and cloned a novel human protein that contains FADD/Mort1 death effector domain homology regions, designated FLAME-1. FLAME-1, although most similar in structure to Mch4 and Mch5, does not possess caspase activity but can interact specifically with FADD, Mch4, and Mch5. Interestingly, FLAME-1 is recruited to the Fas receptor complex and can abrogate Fas/TNFR-induced apoptosis upon expression in FasL/tumor necrosis factor-sensitive MCF-7 cells, possibly by acting as a dominant-negative inhibitor. These findings identify a novel endogenous control point that regulates Fas/TNFR1-mediated apoptosis.
Effects and side effects of a percutaneous thermal lesion of the dorsal root ganglion in patients with cervical pain syndrome (1993)

Twenty consecutive patients with intractable chronic pain in the cervical region were treated with a radiofrequency lesion of the dorsal root ganglion on level C4, C5 or C6. Electromyography (EMG) and sensory evoked potentials (SEP) were recorded before and 3 weeks after the radiofrequency lesion. Side effects were studied 3 weeks, 6 weeks and 3 months after the procedure. Pain scores were evaluated on Numeric Rating Scales (NRS) before and 6 weeks after treatment. The patient was interviewed 3, 6 and 9 months after the radiofrequency lesion. The most common side effect was burning pain in the dermatome of the treated nerve root. Hyposensibility in the dermatome was noticed in 35% of patients. Except in 1 patient, these side effects had disappeared 6 weeks after treatment. The EMG showed no signs of denervation. One SEP recording remained abnormal after treatment. There was initial pain relief in 75% of patients after 3 months and in 50% of the patients after 6 months. In conclusion, this study did not reveal any signs of motor denervation after a percutaneous partial rhizotomy. There were no long-term signs of deafferentation. Initial pain relief was found in 75% of patients, but there was a marked tendency for pain to recur in a period from 3 to 9 months after treatment.
Synthesis of 2'-deoxytubercidin, 2'-deoxyadenosine, and related 2'-deoxynucleosides via a novel direct stereospecific sodium salt glycosylation procedure (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 2'-deoxytubercidin, 2'-deoxyadenosine, and related 2'-deoxynucleosides via a novel direct stereospecific sodium salt glycosylation procedureZygmunt Kazimierczuk, Howard B. Cottam, Ganapathi R. Revankar, and Roland K. RobinsCite this: J. Am. Chem. Soc. 1984, 106, 21, 6379–6382Publication Date (Print):October 1, 1984Publication History Published online1 May 2002Published inissue 1 October 1984https://pubs.acs.org/doi/10.1021/ja00333a046https://doi.org/10.1021/ja00333a046research-articleACS PublicationsRequest reuse permissionsArticle Views1880Altmetric-Citations301LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Growth Arrest and DNA Damage-Inducible Protein GADD34 Assembles a Novel Signaling Complex Containing Protein Phosphatase 1 and Inhibitor 1 (2001)

The growth arrest and DNA damage-inducible protein, GADD34, was identified by its interaction with human inhibitor 1 (I-1), a protein kinase A (PKA)-activated inhibitor of type 1 protein serine/threonine phosphatase (PP1), in a yeast two-hybrid screen of a human brain cDNA library. Recombinant GADD34 (amino acids 233 to 674) bound both PKA-phosphorylated and unphosphorylated I-1(1-171). Serial truncations mapped the C terminus of I-1 (amino acids 142 to 171) as essential for GADD34 binding. In contrast, PKA phosphorylation was required for PP1 binding and inhibition by the N-terminal I-1(1-80) fragment. Pulldowns of GADD34 proteins expressed in HEK293T cells showed that I-1 bound the central domain of GADD34 (amino acids 180 to 483). By comparison, affinity isolation of cellular GADD34/PP1 complexes showed that PP1 bound near the C terminus of GADD34 (amino acids 483 to 619), a region that shows sequence homology with the virulence factors ICP34.5 of herpes simplex virus and NL-S of avian sarcoma virus. While GADD34 inhibited PP1-catalyzed dephosphorylation of phosphorylase a, the GADD34-bound PP1 was an active eIF-2alpha phosphatase. In brain extracts from active ground squirrels, GADD34 bound both I-1 and PP1 and eIF-2alpha was largely dephosphorylated. In contrast, the I-1/GADD34 and PP1/GADD34 interactions were disrupted in brain from hibernating animals, in which eIF-2alpha was highly phosphorylated at serine-51 and protein synthesis was inhibited. These studies suggested that modification of the I-1/GADD34/PP1 signaling complex regulates the initiation of protein translation in mammalian tissues.
MR imaging of laser-tissue interactions. (1988)

A new application of magnetic resonance (MR) imaging to map the spatial and temporal distribution of the effects of Nd:YAG lasers on tissues was studied. The temperature dependence of MR relaxation mechanisms and the high sensitivity of MR to changes in the mobility and distribution of tissue water make it particularly suitable for the demonstration and control of thermal energy deposition in tissues. In heterogeneous tissues, MR imaging does not follow changing temperatures directly because even in the case of reversible thermal interactions, there is a hysteresis in the dynamic relationship between MR signal intensity and temperature. Appropriate matching of the laser and MR pulse sequences can, however, optimize the detection of relatively small laser energy deposition, and reversible and irreversible tissue changes can be distinguished. There is a potential for the integration of MR imaging and lasers for three-dimensional control and monitoring of laser-tissue interactions.
Paxillin α and Crk-associated substrate exert opposing effects on cell migration and contact inhibition of growth through tyrosine phosphorylation (2000)

Protein tyrosine phosphorylation accompanies and is essential for integrin signaling. We have shown that tyrosine phosphorylation of paxillin alpha and Crk-associated substrate (p130(Cas)) is a prominent event on integrin activation in normal murine mammary gland epithelial cells. Tyrosine phosphorylation of p130(Cas) has been demonstrated to facilitate cell migration. We show here that tyrosine phosphorylation of paxillin alpha acts to reduce haptotactic cell migrations as well as transcellular invasive activities in several different experimental cell systems, whereas tyrosine phosphorylation of p130(Cas) exerts opposing effects to those of paxillin alpha. Each of the phosphorylation-null mutants acts as a dominant negative for each phenotype. Moreover, we found that overexpression of paxillin alpha reduced the cell saturation density of normal murine mammary gland cells, whereas overexpression of p130(Cas) increased it. These effects also seemed to depend on tyrosine phosphorylation events. Cell growth rates and morphologies at growing phases were not significantly altered, nor were cells transformed. Addition of epidermal growth factor increased saturation density of the paxillin alpha-overexpressing cells, whereas no further increment was observed in p130(Cas)-overexpressing cells. We propose that tyrosine phosphorylation of paxillin alpha and p130(Cas) exerts opposing effects on several integrin-mediated cellular events, possibly through different signaling pathways.
Premature Atherosclerosis Associated With Monogenic Insulin Resistance (2001)

Rare LMNA mutations that underlie FPLD with insulin resistance and hyperinsulinemia are also associated with early CHD, notably in women. This suggests that abnormalities of the nuclear envelope can result in a phenotype that recapitulates most of the important attributes of the common insulin resistance syndrome, including accelerated cardiovascular disease. FPLD thus appears to be an appropriate human monogenic model for the common insulin resistance syndrome.
BOARD-INVITED REVIEW: The use and application of distillers dried grains with solubles in swine diets (2008)

Distillers dried grains with solubles (DDGS) may be included in diets fed to pigs in all phases of production. The concentrations of DE and ME in DDGS are similar to those in corn. Phosphorus in DDGS is highly digestible to pigs and apparent total tract digestibility values of approximately 60% have been reported. The concentration of starch in DDGS is low (i.e., between 3 and 11%), but the concentration of fat in DDGS is approximately 10% and the concentrations of ADF, NDF, and total dietary fiber in DDGS are approximately 3 times greater than those in corn (9.9, 25.3, and 42.1%, respectively). The apparent total tract digestibility of dietary fiber is less than 50%, which results in reduced digestibility values for DM and energy in DDGS. The concentrations of most AA in DDGS are approximately 3 times greater than those in corn, but the standardized ileal digestibility of most AA is approximately 10 percentage units less than in corn. Nursery pigs from 2 to 3 wk postweaning, and growing and finishing pigs may be fed diets containing up to 30% DDGS without any negative impact on growth performance. However, the carcass fat in pigs fed diets containing DDGS has a greater iodine value than the carcass fat in pigs not fed DDGS. It may, therefore, be necessary to withdraw DDGS from the diet of finishing pigs during the final 3 to 4 wk before slaughter to achieve the desired pork fat quality. Lactating sows can also be fed diets containing up to 30% DDGS, and DDGS can replace all the soybean meal in diets fed to gestating sows without negatively affecting sow or litter performance. Inclusion of DDGS in diets fed to pigs may improve immune system activation, but more research is needed to elucidate the mechanisms responsible for these effects. Manure volume will increase when DDGS is included in the diets because of the reduced digestibility of DM in DDGS. Nitrogen excretion may also increase, but this can be prevented by the use of crystalline AA in diets containing DDGS. In contrast, P excretion can be reduced in diets containing DDGS if the total dietary concentration of P is reduced to compensate for the greater digestibility of P in DDGS. In conclusion, DDGS can be included in diets fed to growing pigs in all phases of production, beginning at 2 to 3 wk postweaning, in concentrations of up to 30% DDGS, and lactating and gestating sows can be fed diets containing up to 30 and 50%, respectively, without negatively affecting pig performance.
Nerve growth factor promotes functional recovery of retinal ganglion cells after ischemia. (1993)

The more proximally located retinal neurons, in particular RGCs, are highly vulnerable to ischemia. Intraocular NGF treatment was effective in enhancing the survival and functional recovery of these neurons. This suggests that NGF may represent a novel therapeutic agent for the treatment of ischemic ocular pathologies.