gebhart/patrepeval huggingface dataset:
IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue (2008)

The stromal-vascular fraction (SVF) of human adipose tissue contains, among other cell types, mesenchymal stem cells and precursors of adipocyte and endothelial cells. Here we show that, in addition, the nonhematopoietic fraction of the SVF has hematopoietic activity, since all types of hematopoietic colony-forming units (CFUs) developed when cultured in methylcellulose-based medium. This hematopoietic activity was restricted to the CD45(-)CD105(+) cell subset, well correlated with KDR(+) cell content, and increased after culture with a combination of early-acting hematopoietic cytokines. Most of the CD45(-)KDR(+)CD105(+) cells were nonadherent and did not express CD31, and this subset included both CD34(-) and CD34(+) cells. Moreover, these nonadherent cells migrated in response to KDR gradient, and when they were cultured in the presence of both hematopoietic and endothelial growth factors, a wave of CFUs was followed by a wave of mixed colonies comprising adherent elongated and nonadherent round hematopoietic cells. These mixed hematopoietic-endothelial (Hem-End) colonies were able to generate secondary Hem-End colonies and exhibited both hematopoietic and endothelial activity, as demonstrated by in vitro functional assays. These findings demonstrate for the first time the existence of primitive mesodermal progenitors within the SVF of human adipose tissue that exhibit in vitro hematopoietic and hemangioblastic activities, susceptible to being used in cell therapy and basic cell research. Disclosure of potential conflicts of interest is found at the end of this article.
Aminoalkohole, XV. Stereoselektive Darstellung und konfigurative Zuordnung der diastereomeren DL‐3‐Amino‐1.2‐diphenyl‐propanole‐(1) (zum Mechanismus der Ringschlußreaktion von Aminoalkoholen mit Benzimidsäureester) (1961)

Abstract 3‐Amino‐1.2‐diphenyl‐propanon‐(1) wird durch Reduktion mit Natriumborhydrid in DL‐ erythro ‐3‐Amino‐1.2‐diphenyl‐propanol‐(1) übergeführt; dessen N ‐Acetylderivat lagert sich beim Kochen mit alkoholischer Salzsäure zum diastereomeren Aminoalkohol um. Die konfigurative Zuordnung stützt sich auf die IR‐Spektren und auf Ringschlußreaktionen, die zu den 2.5.6‐Triphenyl‐5.6‐dihydro‐4 H ‐1.3‐oxazinen führen. – Als Zwischenstufen zur Bildung von substituierten 2‐Phenyl‐5.6‐dihydro‐4 H ‐1.3‐oxazinen bzw. substituierten 2‐Phenyl‐Δ 2 ‐oxazolinen aus Aminoalkoholen und Benzimidsäureester wurden die entsprechenden Amidine erkannt. Es wird ein Mechanismus diskutiert, der diese Zwischenstufe berücksichtigt.
CNS gene expression pattern associated with spontaneous experimental autoimmune encephalomyelitis (2003)

Transgenic mice with T-cell receptor (TCR) specific for myelin basic protein (MBP)-Ac1-11 peptide and homozygous for the RAG-1 mutation (T/R- mice) spontaneously develop acute progressive experimental autoimmune encephalomyelitis (Sp-EAE) mediated by CD4+ T cells. Microarray analysis of spinal cord tissue obtained from symptomatic versus non-symptomatic T/R- mice revealed strongly upregulated transcripts for genes involved in antigen presentation and processing, signal transduction, transcription regulation, metabolism, development, cell cycle, and many other processes involved in the induction of clinical and pathological signs of Sp-EAE. Several highly expressed genes were related directly to inflammation, including cytokines/receptors, chemokines/receptors, acute phase, complement molecules, and others. Many CNS-specific genes were also upregulated in sick mice. Abundance of message for the Tg TCR BV8S2 gene as well as several monocyte/macrophage-associated genes would suggest that both components play a crucial role in the pathogenesis of Sp-EAE. The profile of transcriptional changes found during the development of Sp-EAE provides the first description of the encephalitogenic process in the absence of purposeful immunization with myelin peptides and immune-enhancing adjuvants. This unique approach is the first to implicate molecules and pathways that contribute naturally to onset of paralysis and demyelination, and thus may provide unique insights and novel treatment strategies for human diseases such as multiple sclerosis.
Focal Adhesion Kinase Versus p53: Apoptosis or Survival? (2008)

Focal adhesion kinase (FAK) is a tyrosine kinase that interacts with a multitude of signaling partners and helps cells to survive in the face of various proapoptotic signals. One of the most important interactions for FAK is with the tumor suppressor protein p53. p53 binds not only to the amino-terminal domain of FAK but also to the FAK promoter to inhibit its transcription. A study now reports the biological implications of the kinase-independent interaction of FAK with p53, which opens up future perspectives in cell signaling and cancer research. We focus on FAK and p53 signaling, which link signal transduction pathways from the extracellular matrix and cytoplasm to the nucleus, in human and mouse cells. FAK is proposed to be a critical scaffold protein that sequesters proapoptotic proteins, such as p53, to mediate cell survival.
Janus Microspheres for a Highly Flexible and Impregnable Water‐Repelling Interface (2010)

Janus microspheres composed of superhydrophobic and hydrophilic hemispherical surfaces were prepared using photocurable Pickering emulsion droplets. Upon placement at an air–water interface, an impregnable superhydrophobic barrier with high flexibility is formed. These microspheres have great potential in size-dependent semipermeable membranes, floating micromachines, and superhydrophobic coatings.
Whole-Genome Sequencing in a Patient with Charcot–Marie–Tooth Neuropathy (2010)

As shown in this study of a family with Charcot-Marie-Tooth disease, whole-genome sequencing can identify clinically relevant variants and provide diagnostic information to inform the care of patients.
Expression of the amino-terminal half of the NS1 region of the hepatitis C virus genome and detection of an antibody to the expressed protein in patients with liver diseases (1992)

A cDNA fragment encompassing the 5'-terminal half of the NS1 region of the hepatitis C virus (HCV) genome was cloned. The cDNA was expressed in insect cells using a recombinant baculovirus, and a protein band of approximately 21K was identified by immunoblotting with a serum sample from a patient with chronic hepatitis C. Antibody to the protein was detected in sera from 13.4% of patients with chronic non-A, non-B hepatitis (NANBH), 20.8% of patients with liver cirrhosis and 16.8% of patients with hepatocellular carcinoma with no serum markers for hepatitis B virus infection. However, the antibody was not detected in sera from patients with acute NANBH. The prevalence of antibody to the protein encoded by the NS1 region was lower than that of antibody to the HCV core protein, but much higher than that of antibody to the envelope protein. Thus, the NS1 region of the HCV genome is suggested to encode a protein produced during the course of HCV replication.
Structural features of plant chitinases and chitin‐binding proteins (1994)

Structural features of plant chitinases and chitin-binding proteins are discussed. Many of these proteins consist of multiple domains, of which the chitin-binding hevein domain is a predominant one. X-ray and NMR structures of representatives of the major classes of these proteins are available now, and are used to describe the structures of the other ones. Conserved positions of Cys residues can be taken as evidence for identically located disulfide bridges or cysteine residues. The current classification of chitinases is unsatisfactory and needs to be replaced by an evolutionarily more correct one. As the currently known three-dimensional structures of chitinases are those from barley and the rubber tree, Hevea brasiliensis, it is proposed to adopt the designation b-type (classes I, II and IV) and h-type (classes III and V) chitinases, respectively.
Expression and functional characterisation of System L amino acid transporters in the human term placenta (2015)

LAT1 and LAT2 are present and functional in the syncytiotrophoblast MVM, whereas LAT2 is also expressed in the BM and in the fetal capillary endothelium. In contrast to placental System A and beta amino acid transporters, MVM System L activity is unaffected by maternal overweight/obesity.
Electrochemical and Pulse Radiolysis Study of Misonidazole and Its Azo and Azoxy Derivatives (1984)

Pulse radiolysis experiments were carried out on methanolic solutions of misonidazole and its azo and azoxy derivatives in order to obtain the spectra of their radical anions. The rate constants for the reaction of these compounds with es- were 2.5 X 10(10), 2.3 X 10(10) and 1.9 X 10(10) M-1 s-1 respectively. The electrochemical properties of the compounds were studied with the aim of producing their radical anions by electrolysis in aprotic medium (DMF, 0.1 M TEAP ). The potentials at cathodic peaks were -1.05, -1.10 and -1.18 V for misonidazole, azo and azoxy derivatives, respectively. The decay of the radical anion of misonidazole, which produces nitrite ions, was studied in particular, the corresponding rate constant being 2.8 X 10(-4) s-1. The possibility of using electrochemistry in the study of these transient species is discussed.
Tyrosinase gene mutations associated with type IB ("yellow") oculocutaneous albinism. (1991)

We have identified three different tyrosinase gene mutant alleles in four unrelated patients with type IB ("yellow") oculocutaneous albinism (OCA) and thus have demonstrated that type IB OCA is allelic to type IA (tyrosinase negative) OCA. In an inbred Amish kindred, type IB OCA results from homozygosity for a Pro----Leu substitution at codon 406. In the second family, type IB OCA results from compound heterozygosity for a type IA OCA allele (codon 81 Pro----Leu) and a novel type IB allele (codon 275 Val----Phe). In the third patient, type IB OCA results from compound heterozygosity for the same type IB allele (codon 275 Val----Phe) and a novel type IB OCA allele. In a fourth patient, type IB OCA results from compound heterozygosity for the codon 81 type IA OCA allele and a type IB allele that contains no identifiable abnormalities; dysfunction of this type IB allele apparently results from a mutation either well within one of the large introns or at some distance from the tyrosinase gene. In vitro expression of the Amish type IB allele in nonpigmented HeLa cells demonstrates that the Pro----Leu substitution at codon 406 greatly reduces but does not abolish tyrosinase enzymatic activity, a finding consistent with the clinical phenotype.
Photonic Integrated Circuits Using Heterogeneous Integration on Silicon (2018)

Heterogeneous silicon photonics using wafer bonding is reaching maturity with commercial products for the data center market being shipped in volume. Here we give an overview of recent research in the area showing record device performance by using the best of both worlds: III-V for light generation and Si for guiding the light. Utilizing the flexibility of the heterogeneous silicon platform, narrow-linewidth widely tunable lasers as well as fully integrated mode locked lasers with record pulse powers and pulse duration were demonstrated. The ability to perform multiple die bonding with optimized epitaxially grown layer stacks was used to realize high-performance photonic integrated circuits both for communications and sensing. In addition to III-V materials, nonreciprocal materials such as, for example, Ce:YIG can be bonded, providing additional functionality such as on-chip isolators and reconfigurable on-chip circulators. On-chip isolation will become necessary with the increase in complexity of photonic integrated chips as photonic components such as lasers are sensitive to feedback effects.
Oral Abstracts (2023)

Background: In early 2023, the NASEM released new Dietary Reference Intake (DRI) equations for Energy.Infants were the only group without the use of physical activity to predict energy expenditure (EE), due to the lack of data.The gender-specific, but sole equation predicts EE in children aged 0-2 years.The aim of this analysis is to evaluate the accuracy and precision of the new EE prediction equation and to explore if activity EE or accelerometer-measured activity can improve predictions for infants.Methods: Infants aged 1-3 months (N = 13) completed a seven-day doubly labeled water (DLW) study for total daily EE (TDEE) and wore accelerometers (ActiGraph wGT3X-BT) on the waist and ankle for 7 days.Data were cleaned for non-wear and average hourly raw vector magnitude (VM) was calculated for days with a minimum of 80% wear daily wear.A subsample (N = 6) completed metabolic chamber testing for measurement of sleep energy expenditure (SEE).Activity energy expenditure (AEE) was calculated as TDEE-SEE.Total EE was estimated using the 2023 DRI equations for infants 0-2 years.Results: TDEE measured was not different from predicted (364 ± 72 vs 379 ± 55 kcals/day, p = 0.43).However, the difference ranged À97 to 125 kcals/day.The EE prediction error was positively associated with measured TDEE (r = 0.69, p = À0.009).When DRI equations overestimated EE, AEE tended to be higher (AEE when EE was overestimated: 93 ± 33 vs when underestimated: 45 ± 17 kcals/day, p = 0.06).There was no relationship among accelerometry and prediction error, however, a significant and positive relationship was observed between AEE and ankle VM (r = 0.93, p = 0.02). Conclusions:The new DRI equations can predict TDEE with a mean prediction error of 6% in a modern cohort of infants, yet the precision is limited.This may be explained by the 2 year age range covered by this equation.Infant activity, while low, increases with age and appears to be meaningful in predicting EE.In line with other groups, activity may explain variation in newly published prediction equations for infants.Further refinement of the DRI equation and/or establishment of meaningful accelerometer outcomes for infants are needed and such tools could be used to measure infant activity and EE.Infant EE should be prioritized in obesity and nutrition research to advance our understanding of dietary needs in this vulnerable population.
A facile “one pot” synthesis of 2,9‐disubstituted 8‐azapurin‐6‐ones (3,5‐disubstituted 7‐hydroxy‐3H‐1,2,3‐triazolo[4,5‐d]pyrimidines) (1985)

Abstract A series of title compounds have been synthesized by utilising benzylazide, cyanoacetamide, ethyl or methyl esters of aliphatic or aromatic carboxylic acids and sodium ethoxide as catalyst.
Glomerular hypertrophy in preeclamptic patients with focal segmental glomerulosclerosis. A morphometric analysis. (1999)

These results support the assumption that glomerular hypertrophy that develops during severe toxemic pregnancy plays an important role in the pathogenesis of FSGS lesion and is reversible about 40 days after delivery.
Application of a Bacteriophage Lysin To Disrupt Biofilms Formed by the Animal Pathogen Streptococcus suis (2011)

Bacterial biofilms are crucial to the pathogenesis of many important infections and are difficult to eradicate. Streptococcus suis is an important pathogen of pigs, and here the biofilm-forming ability of 32 strains of this species was determined. Significant biofilms were completely formed by 10 of the strains after 60 h of incubation, with exopolysaccharide production in the biofilm significantly higher than that in the corresponding planktonic cultures. S. suis strain SS2-4 formed a dense biofilm, as revealed by scanning electron microscopy, and in this state exhibited increased resistance to a number of antibiotics (ampicillin, amoxicillin, ciprofloxacin, kanamycin, and rifampin) compared to that of planktonic cultures. A bacteriophage lysin, designated LySMP, was used to attack biofilms alone and in combination with antibiotics and bacteriophage. The results demonstrated that the biofilms formed by S. suis, especially strains SS2-4 and SS2-H, could be dispersed by LySMP and with >80% removal compared to a biofilm reduction by treatment with either antibiotics or bacteriophage alone of less than 20%; in addition to disruption of the biofilm structure, the S. suis cells themselves were inactivated by LySMP. The efficacy of LySMP was not dose dependent, and in combination with antibiotics, it acted synergistically to maximize dispersal of the S. suis biofilm and inactivate the released cells. These data suggest that bacteriophage lysin could form part of an effective strategy to treat S. suis infections and represents a new class of antibiofilm agents.
Site-directed mutagenesis of conserved Thr407, Asp433 and Met464 residues in small subunit of Escherichia coli gamma-glutamyltranspeptidase. (2007)

Sequence comparison showed that residues Thr407, Asp433, and Met464 in the small subunit of Escherichia coli gamma-glutamyltranspeptidase (EcGGT) were conserved in the aligned enzymes. In this study, we further investigated the functional significance of these conserved residues by site-directed mutagenesis. The wild-type and mutant enzymes were overexpressed in the recombinant E. coli M15 cells and purified to near homogeneity by Ni2+-NTA resin. Except M464L, other mutants had shown no GGT activity under enzyme assay conditions and activity staining. Furthermore, mutations on these residues impaired the capability of autocatalytic processing of the enzyme. Based on these observations, it is concluded that these residues play an important role in the enzyme maturation.
The Java Virtual Machine Specification, Java SE 8 Edition (2013)

Written by the inventors of the technology, The Java Virtual Machine Specification, Java SE 7 Edition, is the definitive technical reference for the Java Virtual Machine. The book provides complete, accurate, and detailed coverage of the Java Virtual Machine. It fully describes the invokedynamic instruction and method handle mechanism added in Java SE 7, and gives the formal Prolog specification of the type-checking verifier introduced in Java SE 6. The book also includes the class file extensions for generics and annotations defined in Java SE 5.0, and aligns the instruction set and initialization rules with the Java Memory Model.
Problems in using statistical analysis of replacement and silent mutations in antibody genes for determining antigen‐driven affinity selection (2005)

The analysis of molecular signatures of antigen-driven affinity selection of B cells is of immense use in studies on normal and abnormal B cell development. Most of the published literature compares the expected and observed frequencies of replacement (R) and silent (S) mutations in the complementarity-determining regions (CDRs) and the framework regions (FRs) of antibody genes to identify the signature of antigenic selection. The basic assumption of this statistical method is that antigenic selection creates a bias for R mutations in the CDRs and for S mutations in the FRs. However, it has been argued that the differences in intrinsic mutability among different regions of an antibody gene can generate a statistically significant bias even in the absence of any antigenic selection. We have modified the existing statistical method to include the effects of intrinsic mutability of different regions of an antibody gene. We used this method to analyse sequences of several B cell-derived monoclonals against T-dependent antigens, T-independent antigens, clones derived from lymphoma and amyloidogenic clones. Our sequence analysis indicates that even after correcting for the intrinsic mutability of antibody genes, statistical parameters fail to reflect the role of antigen-driven affinity selection in maturation of many clones. We suggest that, contrary to the basic assumption of such statistical methods, selection can act both for and against R mutations in the CDR as well as in the FR regions. In addition we have identified different methodological difficulties in the current uses of such statistical analysis of antibody genes.
Digital Image Orderless Technology (2002)

Digital image orderless technology is an important one that gurantees state information safety.They are technology of separate storage, hiding and watermark.
Agents Affecting Lipid Metabolism. XVI. The Synthesis of Analogs of trans-1,4-Bis(2-chlorobenzylaminomethyl)cyclohexane1 (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAgents Affecting Lipid Metabolism. XVI. The Synthesis of Analogs of trans-1,4-Bis(2-chlorobenzylaminomethyl)cyclohexane1Leslie G. HumberCite this: J. Med. Chem. 1965, 8, 3, 401–404Publication Date (Print):May 1, 1965Publication History Published online1 May 2002Published inissue 1 May 1965https://pubs.acs.org/doi/10.1021/jm00327a033https://doi.org/10.1021/jm00327a033research-articleACS PublicationsRequest reuse permissionsArticle Views170Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Pulsed High-Intensity Focused Ultrasound Enhances Uptake of Radiolabeled Monoclonal Antibody to Human Epidermoid Tumor in Nude Mice (2008)

This study demonstrates that pulsed-HIFU significantly enhances the delivery of (111)In-MX-B3 in human epidermoid tumors xenografted in nude mice. The results of this pilot study warrant further evaluation of other treatment regimens, such as repeated HIFU exposures for greater delivery enhancement of antibodies labeled with cytotoxic radioisotopes or pulsed-HIFU exposure in addition to a combined therapy of (90)Y-B3 and taxol to enhance the synergistic effect.
Older people and inflammatory bowel disease: a systematic review (2008)

Nurses play an increasingly important role in the assessment and management of inflammatory bowel disease patients. Little is known about factors which could predict poorer psychosocial health and the impact non-intestinal manifestations may have on this in older people with inflammatory bowel disease.