gebhart/patrepeval huggingface dataset:
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. (1981)

The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the addition of avidin-biotin-peroxidase complex, gives a superior result when compared to the unlabeled antibody method. The availability of biotin-binding sites in the complex is created by the incubation of a relative excess of avidin with biotin-labeled peroxidase. During formation of the complex, avidin acts as a bridge between biotin-labeled peroxidase molecules; and biotin-labeled peroxidase molecules, which contains several biotin moieties, serve as a link between the avidin molecules. Consequently, a "lattice" complex containing several peroxidase molecules is likely formed. Binding of this complex to the biotin moieties associated with secondary antibody results in a high staining intensity.
N-(1-Methyl-5-indolyl)-N'-(3-methyl-5-isothiazolyl)urea: A Novel, High-Affinity 5-HT2B Receptor Antagonist (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTN-(1-Methyl-5-indolyl)-N'-(3-methyl-5-isothiazolyl)urea: A Novel, High-Affinity 5-HT2B Receptor AntagonistIan T. Forbes, Graham E. Jones, Olive E. Murphy, Victoria Holland, and Gordon S. BaxterCite this: J. Med. Chem. 1995, 38, 6, 855–857Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/jm00006a001https://doi.org/10.1021/jm00006a001research-articleACS PublicationsRequest reuse permissionsArticle Views398Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (3)»Supporting Information Supporting Information Get e-Alerts
Radioimmunotherapy of prostate cancer in human xenografts using monoclonal antibodies specific to prostate specific membrane antigen (PSMA): Studies in nude mice (2003)

In nude mice bearing PSMA positive tumors, radiation dose to the tumor with (90)Y-DOTA-J591 is greater for large tumors than with (131)I-J591. The theoretical and practical considerations strongly suggest that (90)Y-DOTA-huJ591 may be a suitable radiopharmaceutical for the treatment of prostate cancer.
A day in the life of indigenous Australia: From Flora and Fauna to personhood (2018)

At a 2018 Reconciliation Week talk at the Australian National University ('ANU'), a student commented on how Australia has an enviable human rights record. He noted it was a favoured destination for immigrants from all parts of the world, was an affluent and successful multi-cultural, multi-religious society and enjoyed freedoms that were the envy of world. Most importantly, he also noted that First Nations peoples were recognised at formal events through 'Welcome or Acknowledgement of Country' addresses (including an opening statement made at that particular Reconciliation week function) and seem to enjoy great respect. He rather quizzically asked 'what more' could First Nations people want? The underlying tone of the question was that Indigenous people were ingrate and that the insistence by Indigenous people for 'further' recognition was much more than was reasonably warranted or necessary.
<title>Pyramid AR model to generate fractal Brownian random field</title> (1993)

Fractal Brownian random (FBR) field is an extension of fractional Brownian motion (FBM) and has been successfully used in image analysis, the generation of natural scenes, fractal geometry, and other areas. But its implementation is difficult and limits its applications. In this paper, by extending the AR model, we propose a new approach, the pyramid-AR-model approach, to implement FBR fields. The new method has the same computational complexity as A. Fournier's, but it can generate FBR fields much more accurately.
HMM-based Speech Synthesis Using an Acoustic Glottal Source Model (2011)

Parametric speech synthesis has received increased attention in recent years following the development of statistical HMM-based speech synthesis. However, the speech produced using this method still does not sound as natural as human speech and there is limited parametric flexibility to replicate voice quality aspects, such as breathiness. The hypothesis of this thesis is that speech naturalness and voice quality can be more accurately replicated by a HMM-based speech synthesiser using an acoustic glottal source model, the Liljencrants-Fant (LF) model, to represent the source component of speech instead of the traditional impulse train. Two different analysis-synthesis methods were developed during this thesis, in order to integrate the LF-model into a baseline HMM-based speech synthesiser, which is based on the popular HTS system and uses the STRAIGHT vocoder. The first method, which is called Glottal Post-Filtering (GPF), consists of passing a chosen LF-model signal through a glottal post-filter to obtain the source signal and then generating speech, by passing this source signal through the spectral envelope filter. The system which uses the GPF method (HTS-GPF system) is similar to the baseline system, but it uses a different source signal instead of the impulse train used by STRAIGHT. The second method, called Glottal Spectral Separation (GSS), generates speech by passing the LF-model signal through the vocal tract filter. The major advantage of the synthesiser which incorporates the GSS method, named HTS-LF, is that the acoustic properties of the LF-model parameters are automatically learnt by the HMMs. In this thesis, an initial perceptual experiment was conducted to compare the LFmodel to the impulse train. The results showed that the LF-model was significantly better, both in terms of speech naturalness and replication of two basic voice qualities (breathy and tense). In a second perceptual evaluation, the HTS-LF system was better than the baseline system, although the difference between the two had been expected to be more significant. A third experiment was conducted to evaluate the HTS-GPF system and an improved HTS-LF system, in terms of speech naturalness, voice similarity and intelligibility. The results showed that the HTS-GPF system performed similarly to the baseline. However, the HTS-LF system was significantly outperformed by the baseline. Finally, acoustic measurements were performed on the synthetic speech to investigate the speech distortion in the HTS-LF system. The results indicated that a problem in replicating the rapid variations of the vocal tract filter parameters at transitions between voiced and unvoiced sounds is the most significant cause of speech distortion. This problem encourages future work to further improve the system.
Logistik (1996)

Aufgrund der Vielfalt logistischer Systeme und Prozesse herrscht weder in der Wirtschaftspraxis noch in der Wissenschaft Konsens darüber, was unter dem Begriff Logistik zu verstehen ist.237 Erscheinungsformen und Ausprägungen der Logistik innerhalb oder zwischen den Wirtschaftssubjekten sind so vielfältig und inhomogen wie diese selbst. Entsprechend der Vielfalt realer Unternehmen ist auch das Spektrum der Ausprägungen logistischer Systeme nahezu unüberschaubar.
Effectiveness of Breast Cancer Surveillance in BRCA1/2 Gene Mutation Carriers and Women With High Familial Risk (2001)

PURPOSE: Women with a high breast cancer risk due to a familial predisposition may choose between preventive surgery and regular surveillance. The effectiveness of surveillance in high-risk women and especially BRCA1/2 mutation carriers is unknown. We present first results from a single large family cancer clinic. PATIENTS AND METHODS: Women with breast cancer risk over 15% were examined by physical examination every 6 months and mammography every year. Detection rates and screening parameters were calculated for the total group and separately for different age and genetic risk groups. RESULTS: At least one examination was performed in 1,198 women: 449 moderate and 621 high-risk women and 128 BRCA1/2 mutation carriers. Within a median follow-up of 3 years, 35 breast cancers were detected (four ductal carcinoma-in-situ; 31 invasive tumors); the average detection rate was 9.7 per 1,000. Detection rates (95% confidence interval) for moderate and high-risk women and BRCA1/2 carriers were 3.3 (1.1 to 8.6), 8.4 (5.4 to 13.2), and 33 (17 to 63) per 1,000 person-years, respectively. The ratio of observed cases versus breast cancers expected in an average-risk population of comparable age was 2.7, 7.0 and 23.7 respectively. Overall, node negativity was 65%; 34% of primary tumors were less than 10 mm; sensitivity was 74%. Results with respect to tumor stage and sensitivity were less favorable in BRCA1/2 carriers and in women under the age of 40. CONCLUSION: It is possible to identify young women at high risk for breast cancer. The number of cancers detected was significantly greater than expected in an age-matched average-risk population and related to the risk category. Overall, screening parameters were comparable to population screening data, with less favorable results in the youngest age group (&lt; 40) and BRCA1/2 carriers.
Electrophilic and nucleophilic catalysis of the scission of the sulfur-sulfur bond (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrophilic and nucleophilic catalysis of the scission of the sulfur-sulfur bondJohn L. KiceCite this: Acc. Chem. Res. 1968, 1, 2, 58–64Publication Date (Print):February 1, 1968Publication History Published online1 May 2002Published inissue 1 February 1968https://pubs.acs.org/doi/10.1021/ar50002a004https://doi.org/10.1021/ar50002a004research-articleACS PublicationsRequest reuse permissionsArticle Views588Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Annealing of Starch at an Intermediate Water Content (1991)

Abstract The effect of annealing on starch gelatinization behaviour has earlier been described in the literature. The starch: water ratio in these studies have been either very low or very high. In this work the gelatinization behaviour has been studied both with time and temperature at an intermediate starch/water ratio (1:1) by differential scanning calorimetry. Starches from maize, waxy‐maize, wheat and potato have been compared. This investigation shows that annealing causes an increase of T o and T m , a narrowing of the gelatinization temperature interval, and a change of the shape of the endotherm can also be observed. The annealing time has to be more than 6 h to induce these changes.
Cruciate reconstruction using freeze dried anterior cruciate ligament allograft and a ligament augmentation device (LAD) (1987)

One ACL in each of 11 mature goats was replaced with a freeze dried bone-ACL-bone allograft and a ligament augmentation device (LAD). The LAD was released from its tibial fixation at 3 months postoperation. Biomechanical, microvascular, and histological changes were evaluated 1 year following implantation. The reconstructed knees had a significantly greater total AP translation (3.1 +/- 0.5 mm) (mean and SEM) than the contralateral controls (1.0 +/- 0.1 mm). Differences in primary AP translation were responsible for 59% of the difference in total translation, with only a 0.6 mm difference in secondary translation. Neutral stiffness in the reconstructive knee was 22% of control, while stiffness at 30 N of anterior force was approximately 35% of controls. Ligament stiffness in the linear region for the ACL allograft/LAD was 53% of the control value of 691 N/mm. The maximum load of the allograft/LADs was 1,052 +/- 145 N, or 43% of the contralateral ACL control strength (2,448 +/- 144 N). Five of the six allografts failed at the femoral insertion. Energy (39%) to maximum load was less for allograft/LADs than controls but elongation to maximum load was the same as control. Histologic evaluation of the allograft/LADs revealed soft tissue cellular ingrowth into the LAD in the extraarticular portions. No bony growth into the LAD was observed. The collagen fibers of the graft appear to be arranged in a longitudinal orientation although some areas show chaotic collagen fibers. Microangiography revealed a periligamentous and endoligamentous vascular pattern reminiscent of a normal ACL and complete revascularization of the bone plugs.
Adipose Expression of Tumor Necrosis Factor-α: Direct Role in Obesity-Linked Insulin Resistance (1993)

Tumor necrosis factor-alpha (TNF-alpha) has been shown to have certain catabolic effects on fat cells and whole animals. An induction of TNF-alpha messenger RNA expression was observed in adipose tissue from four different rodent models of obesity and diabetes. TNF-alpha protein was also elevated locally and systemically. Neutralization of TNF-alpha in obese fa/fa rats caused a significant increase in the peripheral uptake of glucose in response to insulin. These results indicate a role for TNF-alpha in obesity and particularly in the insulin resistance and diabetes that often accompany obesity.
Emerging disease modifying therapies for multiple sclerosis (2003)

AbstractMultiple sclerosis (MS), the most common central nervous system (CNS) demyelinating disease, is thought to be mediated in part by CNS autoantigen-specific T cells. The aetiology of the disease is unclear, but includes genetic and environmental factors. The disease onset often occurs in young adults and is characterised by bouts of neurological symptoms such as numbness, weakness, imbalance or visual difficulties that may not be recovered from. Sometimes the course is more progressive. Since the disease can be disabling, several treatments have been developed that reduce the risk of relapse and progression of sustained disability. Although earlier treatment is encouraged, currently approved disease modifying therapies for MS are only partially effective, administered parenterally and associated with significant side effects and potential toxicities. Therefore, many promising new therapies are under development that target various goals, including immunosuppression, immunomodulation, cell traffic through the blood–brain barrier (BBB), neuroprotection and enhancement of CNS repair.Keywordsdisease modifying therapy for MSimmunomodulationimmunosuppressionmultiple sclerosisneuroprotection
A base-excision DNA-repair protein finds intrahelical lesion bases by fast sliding in contact with DNA (2006)

A central mystery in the function of site-specific DNA-binding proteins is the detailed mechanism for rapid location and binding of target sites in DNA. Human oxoguanine DNA glycosylase 1 (hOgg1), for example, must search out rare 8-oxoguanine lesions to prevent transversion mutations arising from oxidative stress. Here we report high-speed imaging of single hOgg1 enzyme molecules diffusing along DNA stretched by shear flow. Salt-concentration-dependent measurements reveal that such diffusion occurs as hOgg1 slides in persistent contact with DNA. At near-physiologic pH and salt concentration, hOgg1 has a subsecond DNA-binding time and slides with a diffusion constant as high as 5 x 10(6) bp(2)/s. Such a value approaches the theoretical upper limit for one-dimensional diffusion and indicates an activation barrier for sliding of only 0.5 kcal/mol (1 kcal = 4.2 kJ). This nearly barrierless Brownian sliding indicates that DNA glycosylases locate lesion bases by a massively redundant search in which the enzyme selectively binds 8-oxoguanine under kinetic control.
Correction for head movements in positron emission tomography using an optical motion tracking system (2002)

Methods capable of correcting for head motion in all six degrees of freedom have been proposed for PET brain imaging but not yet demonstrated in human studies. These methods rely on the accurate measurement of motion in a coordinate frame aligned with the scanner. We present methodology for the direct calibration of an optical motion tracking system to the reconstruction coordinate frame using paired coordinate measurements obtained simultaneously from a PET scanner and tracking system. We also describe the implementation of motion correction, based on the multiple acquisition frame method originally described by Y. Picard and C.J. Thompson (1997), using data provided by the motion tracking system. Effective compensation for multiple six degree-of-freedom movements is demonstrated in dynamic PET scans of the Hoffman brain phantom and a normal volunteer. We conclude that reduced distortion and improved quantitative accuracy can be achieved with this method in PET brain studies degraded by head movements.
Universal network of small wireless operators (UNSWo) (2002)

The recent auctioning of wireless bandwidth in various countries indicates that the service provided by Telcos over these networks will be very expensive. A new type of architecture is currently evolving as a low cost alternative cellular wireless service by allowing the users to play the role of a wireless network operator. Some users of the current Internet may install a low cost base station based on their needs and interconnection between these base stations will form a grid of wireless Internet. This paper provides a survey on related work that either uses ad-hoc mobile networking or a combination of fixed and wireless networking. Finally we present an alternate architecture and describe new issues that arise from this new type of architecture.
Kinetics of prostacyclin synthesis in PGHS-1-overexpressed endothelial cells (1994)

The availability of a human endothelial cell overexpressed with prostaglandin H synthase-1 (PGHS-1) by retrovirus-mediated gene transfer made it possible to quantify the kinetics of prostacyclin [prostaglandin (PG)I2] synthesis and PGHS-1 turnover. Prostacyclin synthesis in response to arachidonate (AA) and ionophore A-23187 fit a single exponential kinetics. The rate constants for AA- and ionophore-treated cells were 0.064 min-1 [half-life (t1/2) of 11 min] and 0.032 min-1 (t1/2 = 22 min), respectively. The rate constant of PGI2 synthesis from PGH2 was 0.13 min-1. Using kinetic analysis coupled with computer modeling, the PGHS-1 half-life was determined to be 10.8 min. PGI2 production under successive treatments with AA or ionophore was reduced by only approximately 30% after each treatment. The decline of PGI2 synthesis corresponded to the reduction of PGHS-1 mass. The half-life of PGI2 synthesis from this analysis was at least an order of magnitude higher than that estimated from the single-dose experiment. These findings indicate that approximately 30% of PGHS-1 was degraded during each catalysis-induced autoinactivation and that the extent and duration of PGI2 synthesis are governed by the level of PGHS-1 mass.
In vivo effects of follicle-stimulating hormone-related synthetic peptides on the mouse estrous cycle. (1996)

We have previously shown that a synthetic peptide amide corresponding to residues 34-37 (TRDL) of the human (h) FSH beta-subunit inhibited binding of [125I]hFSH to bovine calf testis membrane receptors and antagonized FSH-stimulated estradiol biosynthesis in primary cultures of rat Sertoli cells. These in vitro effects would have additional significance if they could be confirmed in an in vivo model system. We have obtained several lines of evidence supporting in vivo effects of TRDL on the mouse estrous cycle. 1) A single i.p. injection of 200 microg/g BW TRDL induced persistent vaginal estrus, characterized by the complete absence of epithelial casts in 87% of the mice treated, as determined by vaginal cytology. 2) A synthetic peptide representing a larger receptor-binding domain of the hFSH beta-subunit, hFSHbeta-(33-53), that contains the TRDL sequence had a similar effect, but hFSHbeta-(38-53) lacking the TRDL sequence, did not. 3) A series of unrelated synthetic peptides, tested at a comparable dose (200 microg/g BW), were also without effect, as was a D-amino acid analog of TRDL, TR(D)DL. 4) Serum estradiol levels at proestrus in TRDL-treated mice were significantly lower than those in vehicle-injected control mice. 5) The effect of estrogen on uterine ballooning and weight gain, seen in all vehicle-injected control mice at proestrus, did not occur in 97% of the mice treated with TRDL. 6) The ovaries of TRDL-treated mice taken during persistent vaginal estrus contained a greater number of large hemorrhagic preovulatory follicles and fewer corpora lutea than those in ovaries taken at estrus from vehicle-injected control mice. Taken together, these results indicate disruption of the normal mouse estrous cycle by the TRDL peptide and represent the first demonstration of in vivo effects of gonadotropin-related synthetic peptides on reproductive processes.
Genome-Wide Array-Based Comparative Genomic Hybridization of Diffuse Large B-Cell Lymphoma (2004)

Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin's lymphoma and exhibits aggressive and heterogeneous clinical behavior. To genetically characterize DLBCL, we established our own array-based comparative genomic hybridization and analyzed a total of 70 cases [26 CD-positive (CD5+) DLBCL and 44 CD5-negative (CD5-) DLBCL cases]. Regions of genomic aberrations observed in >20% of cases of both the CD5+ and CD5- groups were gains of 1q21-q31, 1q32, 3p25-q29, 5p13, 6p21-p25, 7p22-q31, 8q24, 11q23-q24, 12q13-q21, 16p13, 18, and X and losses of 1p36, 3p14, 6q14-q25, 6q27, 9p21, and 17p11-p13. Because CD5 expression marks a subgroup with poor prognosis, we subsequently analyzed genomic gains and losses of CD5+ DLBCL compared with those of CD5-. Although both groups showed similar genomic patterns of gains and losses, gains of 10p14-p15 and 19q13 and losses of 1q43-q44 and 8p23 were found to be characteristic of CD5+ DLBCL. By focusing on the gain of 13q21-q34 and loss of 1p34-p36, we were also able to identify prognostically distinct subgroups among CD5+ DLBCL cases. These results suggest that array-based comparative genomic hybridization analysis provides a platform of genomic aberrations of DLBCL both common and specific to clinically distinct subgroups.