gebhart/patrepeval huggingface dataset:
Carbon nanomaterials field-effect-transistor-based biosensors (2012)

Carbon nanomaterials field-effect transistor (FET)-based electrical biosensors provide significant advantages over the current gold standards, holding great potential for realizing direct, label-free, real-time electrical detection of biomolecules in a multiplexed manner with ultrahigh sensitivity and excellent selectivity. The feasibility of integrating them with current complementary metal oxide semiconductor platform and a fluid handling module using standard microfabrication technology opens up new opportunities for the development of low-cost, low-noise, portable electrical biosensors for use in practical future devices. In this article, we review recent progress in the rapidly developing area of biomolecular interaction detection using FET-based biosensors based on the carbon nanomaterials single-walled carbon nanotubes (SWNTs) and graphene. Detection scenarios include DNA–DNA hybridization, DNA–protein interaction, protein function and cellular activity. In particular, we will highlight an amazing property of SWNT- or graphene-FETs in biosensing: their ability to detect biomolecules at the single-molecule level or at the single-cell level. This is due to the size comparability and the surface compatibility of the carbon nanomaterials with biological molecules. We also summarize some current challenges the scientific community is facing, including device-to-device heterogeneity and the lack of system integration for uniform device array mass production. The detection of biological events is crucial in many life science applications such as disease diagnosis and the analysis of biological systems. Song Liu and Xuefeng Guo from the Beijing National Laboratory for Molecular Sciences and Peking University now review the use of field-effect transistors (FET) based on carbon nanomaterials for biosensing applications. Biosensors made from graphene or carbon nanotubes-based FETs have several advantages. They are very sensitive to environmental parameters such as surface charges or pH values. Furthermore, these nanostructures are very good charge conductors, allowing for a fast response to external changes. In particular sensors based on transistor geometries are of benefit, as biological reactions taking place at their surface directly influence the charge transport through the device. Although reliability remains an issue, such carbon-derived biosensors have already shown protein reactions and detection down to the single molecule level, clearly establishing their potential for practical applications. In this article, we review recent progress in the rapidly developing area of biomolecular interaction detection with excellent selectivity and ultrahigh sensitivity, such as DNA-DNA hybridization, DNA-protein interaction, protein function, and cellular activity, using FET-based biosensors based on the carbon nanomaterials single-walled carbon nanotubes (SWNTs) and graphenes. We also summarize some current challenges the scientific community is facing, including device-to-device heterogeneity and the lack of system integration for uniform device array mass-production.
Modulation of Lysyl Oxidase-like 2 Enzymatic Activity by an Allosteric Antibody Inhibitor (2010)

In this report, we assessed the steady-state enzymatic activity of lysyl oxidase-like 2 (LOXL2) against the substrates 1,5-diaminopentane (DAP), spermine, and fibrillar type I collagen. We find that both DAP and spermine are capable of activating LOXL2 to the same extent and have similar Michaelis constants (K(m) approximately 1 mm) and catalytic rates (k(cat) approximately 0.02 s(-1)). We also show that LOXL2 is capable of being inhibited by a known suicide inhibitor of lysyl oxidase (LOX), beta-aminopropionitrile, which we find is a potent inhibitor of LOXL2 activity. The modality of inhibition of beta-aminopropionitrile was also examined and found to be competitive with respect to the substrates DAP and spermine. In addition, we identified an antibody inhibitor (AB0023) of LOXL2 enzymatic function and have found that the inhibition occurs in a non-competitive manner with respect to both spermine and DAP. The binding epitope of AB0023 was mapped to the scavenger receptor cysteine-rich domain four of human LOXL2. AB0023 binds to a region remote from the catalytic domain making AB0023 an allosteric inhibitor of LOXL2. This affords AB0023 several advantages, because it is specific for LOXL2 and inhibits the enzymatic function of LOXL2 in a non-competitive manner thereby allowing inhibition of LOXL2 regardless of substrate concentration. These results suggest that antibody allosteric modulators of enzymatic function represent a novel drug development strategy and, in the context of LOXL2, suggest that inhibitors such as these might be useful therapeutics in oncology, fibrosis, and inflammation.
New Oral Dosage Form for Elderly Patients: Preparation and Characterization of Silk Fibroin Gel. (1995)

The pharmaceutical utility of silk fibroin as a possible material for an oral dosage form for elderly patients was investigated. Silk fibroin gel (SFG) was prepared from its aqueous solution. The gel formation was studied as a function of adjusted pH and concentration of silk fibroin (SF). On the basis of Fourier transform infrared spectroscopy of SFG, the transition from the random coil to the beta-structure was observed. The rate of gelation was sufficiently accelerated by the addition of glycerol to the SF aqueous solution. The glycerol content also affected the rate of gelation of the SF solution. Rheological properties of SFG were evaluated using a creep meter. The SF content and/or glycerol content affected the breaking stress of SFG. Moisture desorption from SFG was retarded with an increase in glycerol content. It was found that SFG was able to be prepared at room temperature (20 +/- 5 degrees C), and the SF content and glycerol content affected the formation and physicochemical properties of SFG.
Using "Basic Principles" To Understand Complex Science: Nicotine Smoke Chemistry and Literature Analogies (2005)

The Henderson–Hasselbalch equation calculates the equilibrium distribution of 50:50 for nicotine in its nonprotonated (free base form), relative to its monoprotonated form, at pH of 8 in dilute aqueous solution. This ratio has then been used in the literature to predict the effect of ammonia compounds in tobacco and in smoke on nicotine pyrolysis and smoke chemistry. Experiments demonstrate that neither the thermal chemistry of tobacco alkaloids nor the transfer of nicotine from tobacco to smoke can be explained by the position of the nonprotonated versus monoprotonated form equilibrium in aqueous extracts of tobacco. The high thermal stability of nicotine in air allows nicotine salts to be converted to nonprotonated nicotine and volatilize during heating prior to any substantial decomposition of the nicotine moiety. In contrast, cocaine hydrochloride is thermally unstable and will rapidly decompose upon heating; cocaine hydrochloride must first be converted to its nonprotonated form prior to heating and volatilization.
Reducing the peak to average power ratio of multicarrier signals by adaptive subcarrier selection (2002)

The non-constant envelope is a fundamental problem of the OFDM multicarrier technique. In many cases, nonlinear power amplifiers with power saturation lead to a high out-of-band radiation. Another characteristic of OFDM is the influence of frequency selective fading of mobile radio channels. To overcome this effect coding schemes are required for low error rates, therefore a certain redundancy has to be introduced. This paper presents a combination of an adaptive subcarrier selection as a coding scheme with a new algorithm to reduce the out-of-band radiation, exploiting the non-information carrying subcarriers. The efficiency of this technique is demonstrated by the simulation results.
Molecular detection of minimal residual disease in colorectal and breast cancer (1999)

Recent developments in the field of molecular biology enable us to detect tumour cells at a submicroscopical level. In colorectal and breast cancer the most important prognostic factor is dissemination of malignant cells to locoregional lymph nodes. An important issue is whether molecular 'super'-staging augments the accuracy by which the prognosis of individual patients can be assessed. Over the past few years numerous studies have reported the use of different PCR-based techniques in various types of cancer. The reported incidence of micrometastases and specificity of different assays varies tremendously. This clearly indicates the need for uniformity in protocols. For colorectal cancer the use of molecular techniques may improve staging and guide clinical decisions. For breast cancer there is still need to prove the clinical implication of finding occult metastatic disease. Nevertheless, PCR-based techniques are a powerful tool in the staging of common solid tumours and are likely to find their way into the daily practice of diagnostic histopathologists in the near future.
Using the Intrinsic Chirality of a Molecule as a Label-Free Probe to Detect Molecular Adsorption to a Surface by Second Harmonic Generation (2005)

Chiral second harmonic generation (C-SHG) has been used for the label-free detection of (R)-(+)-1,1'-bi-2-naphthol (RBN) and (S)-(+)-1,1'-bi-2-naphthol (SBN) binding to planar-supported lipid bilayers of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphotidylcholine (POPC) based on the intrinsic chirality of the molecules. C-SHG adsorption isotherms of RBN and SBN reveal Langmuir adsorption behavior with binding constants of 2.7 +/- 0.2 x 10(5) M(-1) and 3.0 +/- 0.1 x 10(5) M(-1), respectively. The kinetics of RBN binding to a POPC bilayer was also measured. It was determined that the adsorption rate for RBN was 5.7 +/- 0.4 x 10(3) s(-1)M(-1) and the desorption rate was 2.1 +/- 0.8 x 10(-2) s(-1). From the kinetic data a binding constant of 2.7 +/- 1.0 x 10(5) M(-1) was calculated, which agrees well with the thermodynamic measurement. The C-SHG technique was correlated with surface tension measurements in order to determine the RBN surface excess within the POPC membrane. The maximum surface excess of RBN in a monolayer of POPC was 4.3 +/- 0.5 x 10(-11) mol cm2. Using the maximum surface excess in conjunction with the C-SHG binding data a lower limit of detection of 1.5 +/- 0.1 x 10(-13) mols cm(-2) was calculated. The results of these studies show that C-SHG is a powerful tool for the study of chiral molecular interactions at surfaces.
Biomimetic Design and Performance of Polymerizable Lipids (2009)

Bilayer lipid membranes (BLMs) have received significant attention over the past several decades because of their applications in biological and material sciences. BLMs consist of two amphiphilic lipid layers arranged with their hydrophilic head region exposed to the surrounding aqueous environment and hydrophobic domains in the core. In biology, lipid membranes confine and support the cell structure while selectively controlling the diffusion of ions and proteins between the intra- and extracellular matrix (ECM). Naturally derived lipid monomers spontaneously self-assemble to develop smart gateways that recognize and incorporate desired protein transporters or ion channels. BLMs are useful research models of lamellar lipid assemblies and associated protein receptors in cell membranes. The transport properties of lipid membranes can be tuned through careful consideration of the solution medium, transporter functionality, and pH, as well as other environmental conditions. BLMs are of particular interest in the design of biofunctional coatings, controlled release technologies, and biosensors; however, high-performance applications require lipid membranes to remain stable under harsh denaturing conditions. Accordingly, synthetic strategies are often proposed to increase the chemical and mechanical stability of lipid assemblies. The polymerization of self-assembled lipid structures is a strategy that results in robust biocompatible architectures, and diverse reactive functional groups are available for the synthesis of monomeric lipids. The selection of the polymerizable functionality and its precise location within the lipid assembly influences the ultimate supramolecular microstructure and polymerization efficiency. The biomimetic potential of polymerized lipids depends on the stability and robustness of the self-assembled membranes, and it is essential that the polymerizable functionality not disturb the amphiphilic nature of the lipid to maintain biocompatibility. Innovative applications are the motivational force for the development of durable polylipid compositions. Surface modification with biocompatible polylipids provides the opportunity for specific binding of biological molecules for applications as sensors or controlled release delivery vehicles. The ability to create stable lipid assemblies requires a comprehensive understanding of the mechanism of lipid polymerization in confined supramolecular geometries. The future is exciting as researchers begin to fully understand the morphology of polylipids in an effort to successfully produce naturally derived sustainable materials. In this Account, we highlight recent efforts to covalently stabilize lipid membranes and discuss emerging applications of mechanically robust self-assembled lipid architectures.
공학설계 교육과정의 설계방식 분석 -P대학 공학설계 교육과정을 중심으로- (2007)

본 연구에서는 교육과정 설계모형에 기초하여 기계공학부의 전공교육과정 중 공학설계 교육과정의 설계방식을 분석하고자 하였다. 이를 위하여 Drake & Burns(2004)가 제시한 교육과정 조직요소간의 관계모형과 Toohey(1999)의 대학교육과정 설계유형을 분석의 틀로 활용하였다. 교육과정의 조직요소간의 관계와 설계유형을 분석한 결과는 다음과 같다. 첫째, 교육과정 조직요소간의 관계는 각 과목의 교육목표를 달성하기 위하여, 설계의 구체적 정보, 주제와 주요 개념의 순서로 관련된 지식을 습득하고, 공학설계에 관련된 기초기능, 범학문적 기능과 고급 기능을 순차적으로 향상시킬 수 있게 설계되어 있다. 둘째, 분석대상인 창의적 공학설계, 설계 및 제작과제와 졸업설계 및 제작과제의 교육과정 설계유형은 프로젝트, 탐구, 문제 중심설계의 형태를 취하고 있다. 본 연구결과는 공학설계 교육과정의 교육내용들 간의 연계성을 검토하기 위한 기초 자료로 활용될 수 있을 것이다.
Low band gap conjugated small molecules containing benzobisthiadiazole and thienothiadiazole central units: synthesis and application for bulk heterojunction solar cells (2011)

Two novel conjugated low band gap small molecules (SMs), M1 and M2, containing benzobisthiadiazole and thienothiadiazole central units, respectively, were synthesized. Both SMs carried terminal cyanovinylene 4-nitrophenyl at both sides which were connected to the central unit with a thiophene ring. The long-wavelength absorption band was located at 591–643 nm and the optical band gap was 1.62–1.63 eV, which is lower than that of P3HT. These two SMs were investigated as electron donor materials along with PCBM or F as electron acceptors for fabrication of bulk heterojunction (BHJ) organic photovoltaic devices. F is a modified PCBM which has been previously synthesized and used as an electron acceptor for poly(3-hexylthiophene) (P3HT). The power conversion efficiency (PCE) for M1:PCBM, M1:F, M2:PCBM and M2:F was 1.05%, 2.02%, 1.23% and 2.72%, respectively. The higher PCE for the devices with M2 as the electron donor has been related to the improved hole mobility for M2. However, the improved PCE for the devices with F as the electron acceptor has been attributed to the more intense absorption of F in the visible region than that of PCBM and also to the higher open circuit voltage resulting from the higher LUMO level of F. We have also fabricated devices with M2:F cast film from mixed solvents. The PCE for the BHJ devices with the as cast and thermally annealed M2:F (mixed solvents) is 3.34% and 3.65%, respectively.
A case control study of cardiovascular health in chemical war disabled Iranian victims (2010)

Cardiovascular abnormalities are another late complication in chemical war disabled Iranian victims. Diastolic dysfunction was the most common abnormality in both groups of patients.
An improved instrument for measuring time-resolved lanthanide emission and resonance energy transfer (1999)

Time-resolved fluorescence, particularly of millisecond lanthanide emission, is of considerable interest because of potentially increased sensitivity when short-lived autofluorescence is a problem. Lanthanides have also previously been shown to be excellent donors in resonance energy transfer measurements, where a lanthanide donor transfers energy to a conventional fluorophore. An instrument for the detection of micro- to millisecond lanthanide luminescence and delayed fluorescence, amidst a background of large prompt fluorescence, is described. Time-delayed emission spectra can be measured with 16 μs dead time while suppressing prompt fluorescence by 25 000-fold. This is accomplished by using a mechanical chopper placed very close to the entrance slit of a diffraction spectrometer, and a charge coupled device detector at the exit port. Excited-state lifetimes can also be measured with a 22 μs dead time while reducing detector ringing due to prompt fluorescence to an insignificant level. This is accomplished by high-voltage gating of a photon-counting photomultiplier tube in combination with a fast constant-fraction discriminator and amplifier. These improvements, which utilize all commercially available equipment, reduce the dead time by a factor of 10 for delayed emission measurements and a factor of 5 for lifetime measurements over a previously described instrument. The current instrument is particularly well suited for lanthanide-based resonance energy transfer measurements.
Optimization of neural network structure and learning parameters using genetic algorithms (2005)

Neural network models of semiconductor manufacturing processes offer advantages in accuracy and generalization over traditional methods. However, model development is complicated by the fact that backpropagation neural networks contain several adjustable parameters whose optimal values are initially unknown. These include learning rate, momentum, training tolerance, and the number of hidden layer neurons. This paper investigates the use of genetic algorithms (GAs) to determine the optimal neural network parameters for modeling plasma-enhanced chemical vapor deposition (PECVD) of silicon dioxide films. To find an optimal parameter set for the PECVD models, a performance matrix is defined and used in the GA objective function. This index accounts for both prediction error as well as training error, with a higher emphasis on reducing prediction error. Results of the genetic search are compared with a similar search using the simplex algorithm. The GA search performed approximately 10% better in reducing training error and 66% better in reducing prediction error.
Autologous Chondrocyte Implantation Compared with Microfracture in the Knee (2004)

Therapeutic study, Level I-1a (randomized controlled trial [significant difference]). See Instructions to Authors for a complete description of levels of evidence.
Related fibroblast growth factor receptor genes exist in the human genome. (1990)

We have isolated, from a human tumor cDNA library, a gene encoding a putative receptor-like protein-tyrosine kinase that we call TK14. The amino acid sequence of the TK14 protein is closely related to the available partial sequence of the mouse protein bek, and more distantly related to the sequences of a chicken basic fibroblast growth factor receptor (73% sequence homology) and the apparent human equivalent of this receptor, the FLG protein (encoded by the fms-like tyrosine kinase gene). Overexpression of the TK14 protein by transfection of COS-1 cells with the corresponding cDNA in a simian virus 40-based expression vector leads to the appearance of new cell-surface binding sites for both acidic and basic fibroblast growth factors. This has been demonstrated by specific binding assays and chemical cross-linking experiments using 125I-labeled growth factors. It appears, therefore, that the human genome contains at least two distinct genes, for TK14 and FLG, that code for related fibroblast growth factor receptors.
Long-term treatment of rosacea with oral tetracycline. (1969)

No abstract available
A receptor for interleukin 10 is related to interferon receptors. (1993)

We isolated cDNAs encoding a mouse interleukin 10 receptor (mIL-10R) from mouse mast cell and macrophage cell lines. The two cDNAs are substantially identical and express an approximately 110-kDa polypeptide in COS7 cells, which binds mIL-10 specifically. A mouse pro-B-cell line (Ba/F3) expressing transfected recombinant mIL-10R binds IL-10 with high affinity (approximately 70 pM) and proliferates in response to mIL-10. mIL-10R is structurally related to interferon receptors (IFNRs). Since IL-10 inhibits macrophage activation by IFN-gamma, a possible implication of this relationship interaction of IL-10R and IFN-gamma R or their signaling pathways.
Association between functional polymorphisms in genes involved in the MAPK signaling pathways and cutaneous melanoma risk (2013)

Genome-wide association studies (GWASs) have mainly focused on top significant single nucleotide polymorphisms (SNPs), most of which did not have clear biological functions but were just surrogates for unknown causal variants. Studying SNPs with modest association and putative functions in biologically plausible pathways has become one complementary approach to GWASs. To unravel the key roles of mitogen-activated protein kinase (MAPK) pathways in cutaneous melanoma (CM) risk, we re-evaluated the associations between 47 818 SNPs in 280 MAPK genes and CM risk using our published GWAS dataset with 1804 CM cases and 1026 controls. We initially found 105 SNPs with P ≤ 0.001, more than expected by chance, 26 of which were predicted to be putatively functional SNPs. The risk associations with 16 SNPs around DUSP14 (rs1051849) and a previous reported melanoma locus MAFF/PLA2G6 (proxy SNP rs4608623) were replicated in the GenoMEL dataset (P < 0.01) but failed in the Australian dataset. Meta-analysis showed that rs1051849 in the 3' untranslated regions of DUSP14 was associated with a reduced risk of melanoma (odds ratio = 0.89, 95% confidence interval: 0.82-0.96, P = 0.003, false discovery rate = 0.056). Further genotype-phenotype correlation analysis using the 90 HapMap lymphoblastoid cell lines from Caucasians showed significant correlations between two SNPs (rs1051849 and rs4608623) and messenger RNA expression levels of DUSP14 and MAFF (P = 0.025 and P = 0.010, respectively). Gene-based tests also revealed significant SNPs were over-represented in MAFF, PLA2G6, DUSP14 and other 16 genes. Our results suggest that functional SNPs in MAPK pathways may contribute to CM risk. Further studies are warranted to validate our findings.
Performance evaluation of a decoded instruction cache for variable instruction-length computers (1992)

A Decoded INstruction Cache (DINC) serves as a buffer between the instruction decoder and the other instruction-pipeline stages. In this paper we explain how techniques that reduce the branch penalty based on such a cache, can improve CPU performance. We analyze the impact of some of the design parameters of DINCs on variable instruction-length computers, e.g., CISC machines.