gebhart/patrepeval huggingface dataset:
Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70 (1991)

Many of the changes in gene expression observed when Escherichia coli cells enter stationary phase are regulated at the level of transcription initiation. A group of stationary-phase-inducible promoters, known as "gearbox" promoter, display a characteristic sequence in the -10 region which differs greatly from the consensus sequence for sigma 70-dependent promoters. Here we describe our studies on the gearbox promoters bolAp1 and mcbAp, responsible for the temporally regulated transcription of bolA and the genes involved in the synthesis of the peptide antibiotic microcin B17, respectively. Deletion analysis of mcbAp demonstrated that the stationary-phase-inducible properties of this promoter are found in a DNA fragment extending from -54 to +11 bp, surrounding the transcriptional start site, and are separable from DNA sequences responsible for the OmpR-dependent stimulation of transcription of mcbAp. In vitro transcription studies indicate that the RNA polymerase holoenzyme involved in the transcription of mcbAp contains sigma 70. In this and an accompanying paper (R. Lange and R. Hengge-Aronis, J. Bacteriol. 173: 4474-4481, 1991), experiments are described which show that the product of katF, a global regulator of stationary-phase gene expression and a putative sigma factor, is required for the expression of bolAp1 fused to the reporter gene lacZ. In contrast, mcbAp appears to be negatively regulated by katF. We discuss the implications of these results for postexponential gene expression and the role of gearbox sequences in the regulation of promoter activity.
Regioselective Oxygenations of S-Trans Dienes, Silyl Dienol Ethers (SDEs), by Triphenyl Phosphite Ozonide (TPPO) and Its Mechanistic Study (2001)

The direct oxygenation of s-trans dienes, silyl dienol ethers (SDEs) 2, by triphenyl phosphite ozonide (TPPO) has been examined in detail. The regioselective oxygenation was found to give hydroperoxide 3, alcohol 4, ketone 5, dimer 6, and peroxy phosphate 7 with concomitant formation of triphenyl phosphate 8 and diphenyl trimethylsilyl phosphate 10. The formation of peroxy phosphate 7 was found for the first time in TPPO oxygenation reactions. The low temperature (31)P and (1)H NMR spectroscopic analyses proved the direct reaction of SDEs with TPPO without generation of singlet oxygen. The formation of the oxygenated products 3-7 is reasonably explained by the intervention of the zwitterion ZI, which can be formed by the nucleophilic attack of SDE to the central oxygen of the ozonide. The regioselective attack of SDE to the central oxygen of the ozonide was supported by the quantum chemical calculation (B3LYP/6-31G).
Vascular Endothelial Growth Factor Receptors VEGFR-2 and VEGFR-3 Are Localized Primarily to the Vasculature in Human Primary Solid Cancers (2010)

VEGFR-2 and VEGFR-3 are primarily localized to, and significantly upregulated on, tumor vasculature (blood and/or lymphatic) supporting the majority of solid cancers. The primary clinical mechanism of action of VEGF signaling inhibitors is likely to be through the targeting of tumor vessels rather than tumor cells. The upregulation of VEGFR-3 on tumor blood vessels indicates a potential additional antiangiogenic effect for dual VEGFR-2/VEGFR-3-targeted therapy.
Can D.S.A. be improved? — Complexity trade-offs with the digital signature standard — (1995)

The Digital Signature Algorithm (DSA) was proposed in 1991 by the US National Institute of Standards and Technology to provide an appropriate core for applications requiring digital signatures. Undoubtedly, many applications will include this standard in the future and thus, the foreseen domination of DSA as a legal certification tool is sufficiently important to focus research endeavours on the suitability of this scheme to various situations. In this paper, we present six new DSA-based protocols for: All our schemes combine in a natural way full DSA compatibility and flexible trade-offs between computational complexity, transmission overheads and key sizes.
Vibrio cholerae-Induced Cellular Responses of Polarized T84 Intestinal Epithelial Cells Are Dependent on Production of Cholera Toxin and the RTX Toxin (2001)

To study the utility of in vitro-polarized intestinal cell monolayers for modeling Vibrio cholerae-host cell interactions, we added live V. cholerae bacteria to the apical surfaces of polarized T84 cell monolayers and monitored changes in electrical properties. We found that both classical and El Tor strains produce cholera toxin after addition to the monolayer, but induction is most likely due to medium components rather than bacterium-cell interactions. We also found that the RTX toxin is produced by El Tor strains. This toxin caused a loss of the barrier function of the paracellular tight junction that was measured as a decrease in transepithelial resistance. This decrease occurred when bacteria were added to either the apical or basolateral surfaces, indicating that the RTX toxin receptor is expressed on both surfaces. These results are discussed with regard to the applicability of the polarized T84 cell monolayers as an in vitro model of host-pathogen interactions.
Spectrum analysis of motion parallax in a 3D cluttered scene and application to egomotion (2005)

Previous methods for estimating observer motion in a rigid 3D scene assume that image velocities can be measured at isolated points. When the observer is moving through a cluttered 3D scene such as a forest, however, pointwise measurements of image velocity are more challenging to obtain because multiple depths, and hence multiple velocities, are present in most local image regions. We introduce a method for estimating egomotion that avoids pointwise image velocity estimation as a first step. In its place, the direction of motion parallax in local image regions is estimated, using a spectrum-based method, and these directions are then combined to directly estimate 3D observer motion. There are two advantages to this approach. First, the method can be applied to a wide range of 3D cluttered scenes, including those for which pointwise image velocities cannot be measured because only normal velocity information is available. Second, the egomotion estimates can be used as a posterior constraint on estimating pointwise image velocities, since known egomotion parameters constrain the candidate image velocities at each point to a one-dimensional rather than a two-dimensional space.
Electrochemical Methoxylation of an HF-Etched Porous Silicon Surface (1998)

The electrochemical methoxylation of a porous silicon (PS) surface using an anhydrous methanol etch and the resulting modification of the photoluminescent emission (PL) from the surface are evaluated within the framework of the silanone-based silicon oxyhydride fluorophors and their methylated counterparts. The molecular electronic structure of the ground-state singlet and low-lying triplet electronic states of some 20 oxyhydrides of the form OSi(R)2, OSi(R)OR, and OSi(OR)2, where R = H, CH3, or SiH3, is considered. The predicted electronic transitions correlate well with the experimentally observed PL for PS and its methoxylated counterpart. The substitution of a methyl (CH3) group for a silyl (SiH3) unit is predicted to produce the observed PL blue shift accompanied by an increased PL intensity and stability.
Immunogene therapy of tumors with vaccine based onXenopushomologous vascular endothelial growth factor as a model antigen (2001)

Overcoming immune tolerance of the growth factors associated with tumor growth should be a useful approach to cancer therapy by active immunity. We used vascular endothelial growth factor (VEGF) as a model antigen to explore the feasibility of the immunogene tumor therapy with a vaccine based on a single xenogeneic homologous gene, targeting the growth factors associated with angiogenesis. To test this concept, we constructed a plasmid DNA encoding Xenopus homologous VEGF (XVEGF-p) and control vectors. We found that immunogene tumor therapy with a vaccine based on XVEGF was effective at both protective and therapeutic antitumor immunity in several tumor models in mice. VEGF-specific autoantibodies in sera of mice immunized with XVEGF-p could be found in Western blotting analysis and ELISA assay. The purified immunoglobulins were effective at the inhibition of VEGF-mediated endothelial cell proliferation in vitro, and at antitumor activity and the inhibition of angiogenesis by adoptive transfer in vivo. The elevation of VEGF in the sera of the tumor-bearing mice could be abrogated with XVEGF-p immunization. The antitumor activity and production of VEGF-specific autoantibodies, significantly elevated IgG1 and IgG2b, could be abrogated by the depletion of CD4(+) T lymphocytes. The observations may provide a vaccine strategy for cancer therapy through the induction of autoimmunity against the growth factors associated with tumor growth in a cross reaction with single xenogeneic homologous gene and may be of importance in the further exploration of the applications of other xenogeneic homologous genes identified in human and other animal genome sequence projects in cancer therapy.
Open-chain poly(organophosphazenes). Synthesis and properties (1998)

Various methods for the synthesis of open-chain poly(organophosphazenes) are considered. The mechanism of polymerisation of hexachlorocyclotriphosphazene and the basic principles of formation of poly(organophosphazene) macromolecules by polymeranalogous reactions of poly(dichloro-phosphazene) with various nucleophilic reagents are analysed from a new viewpoint. The potential of this synthetic method for targeted design of poly(organophosphazenes) of various structures is shown. The possibility of synthesising poly(organo-phosphazenes) by polymerisation of cyclophosphazenes is also discussed. The problem of unit non-uniformity of poly(organophosphazenes) and its influence on the properties of these polymers are considered. The properties of poly(organo-phosphazenes) are considered in detail and it is shown that these polymers possess unusual valuable properties, which provide opportunities for their successful practical application. The bibliography includes 276 references. Various methods for the synthesis of open-chain poly(organophosphazenes) are considered. The mechanism of polymerisation of hexachlorocyclotriphosphazene and the basic principles of formation of poly(organophosphazene) macromolecules by polymeranalogous reactions of poly(dichlorophosphazene) with various nucleophilic reagents are analysed from a new viewpoint. The potential of this synthetic method for targeted design of poly(organophosphazenes) of various structures is shown. The possibility of synthesising poly(organophosphazenes) by polymerisation of cyclophosphazenes is also discussed. The problem of unit non-uniformity of poly(organophosphazenes) and its influence on the properties of these polymers are considered. The properties of poly(organophosphazenes) are considered in detail and it is shown that these polymers possess unusual valuable properties, which provide opportunities for their successful practical application. The bibliography includes 276 references.
Erythrocyte ankyrin: immunoreactive analogues are associated with mitotic structures in cultured cells and with microtubules in brain. (1981)

Human erythrocyte ankyrin, the membrane attachment protein for spectrin, has been detected by radioimmunoassay in a variety of cells and tissues. This report identifies polypeptides crossreacting with ankyrin in brain and HeLa cells and demonstrates that one function of these ankyrin analogues involves association with microtubules. Ankyrin immunoreactivity was localized by indirect immunofluorescence in a colchicine- and detergent-sensitive cytoplasmic meshwork in interphase cells. There also was specific nuclear staining, localized in a bright spots, which was displaced entirely by ankyrin or by high molecular weight microtubule-associated proteins (MAPs) from brain. In dividing cells, the punctate nuclear staining and the meshwork disappeared. Fluorescence was localized at the spindle pole during metaphase and was redistributed to the cleavage furrow in later stages of mitosis. An immunoreactive Mr 370,000 polypeptide comigrating with MAP1 was identified in brain extracts and copolymerized with microtubules through repeated cycles of polymerization and depolymerization. Finally, erythrocyte ankyrin associated with microtubules prepared from pure tubulin, and this binding was displaced by brain MAPs.
Haemopoietic stem/progenitor cell transplant in Fanconi anaemia using HLA‐matched sibling umbilical cord blood cells (1993)

There have only been a few reports documenting the use of umbilical cord blood as a source of stem cells for haemopoietic reconstitution. We report our experience with a child with Fanconi anaemia (FA) who underwent a stem cell transplant using umbilical cord blood cells from his HLA matched sibling. Although the engraftment was somewhat slow, it was complete and comparable to other transplants performed in FA patients using HLA matched sibling marrow. There was no graft-versus-host disease. The post-transplant period was uncomplicated and, at a follow-up of 36 months, this child is well with normal blood counts and immune function.
An efficient algorithm to estimate the instantaneous SNR of speech signals (1993)

This contribution presents an efficient algorithm to estimate the instantaneous signal-to-noise ratio of speech signals. The algorithm is capable to track non stationary noise signals and has a low computational complexity. It does not need a speech activity detector nor histograms to learn signal statistics. The algorithm is based on the observation that a noise power estimate can be obtained using minimum values of a smoothed power estimate. This paper will present this algorithm, its performance, its limits, and some applications.
Energy-Aware Design of Compressed Sensing Systems for Wireless Sensors Under Performance and Reliability Constraints (2013)

This paper describes the system design of a compressed sensing (CS) based source encoding system for data compression in wireless sensor applications. We examine the trade-off between the required transmission energy (compression performance) and desired recovered signal quality in the presence of practical non-idealities such as quantization noise, input signal noise and channel errors. The end-to-end system evaluation framework was designed to analyze CS performance under practical sensor settings. The evaluation shows that CS compression can enable over 10X in transmission energy savings while preserving the recovered signal quality to roughly 8 bits of precision. We further present low complexity error control schemes tailored to CS that further reduce the energy costs by 4X as well as diversity scheme to protect against burst errors. Results on a real electrocardiography (EKG) signal demonstrate 10X in energy reduction and corroborate the system analysis.
Cloning of the cDNA for human IFN-γ-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein (1996)

We have recently reported that a novel molecule, murine IFN-gamma-inducing factor (IGIF) produced by mouse liver cells, possesses potent biologic activities, including the induction of IFN-gamma production by spleen cells and the enhancement of NK cell cytotoxicity. In this paper, we report on the isolation of human IGIF cDNA clones from normal human liver cDNA libraries using murine IGIF cDNA as a probe. The amino acid sequence deduced from the human cDNA clones indicated a 193-amino acid precursor peptide and revealed 65% homology with that of murine IGIF. The amino acid sequence of IGIF also included an IL-1 signature-like sequence. Subsequently, the cloned cDNA was expressed in Escherichia coli, and preliminary studies on the biologic activities of the recombinant protein were performed. The recombinant human IGIF induced IFN-gamma production by mitogen-stimulated PBMC and enhanced NK cell cytotoxicity, in a manner similar to murine IGIF. In addition, recombinant human IGIF also augmented granulocyte-macrophage-CSF production and decreased IL-10 production, but had no effect on IL-4 production by Con A-stimulated PBMC. Based on these pleiotropic effects of IGIF, we propose that this novel cytokine be designated as IL-18.
Compensatory Enlargement of Human Atherosclerotic Coronary Arteries (1987)

Whether human coronary arteries undergo compensatory enlargement in the presence of coronary disease has not been clarified. We studied histologic sections of the left main coronary artery in 136 hearts obtained at autopsy to determine whether atherosclerotic human coronary arteries enlarge in relation to plaque (lesion) area and to assess whether such enlargement preserves the cross-sectional area of the lumen. The area circumscribed by the internal elastic lamina (internal elastic lamina area) was taken as a measure of the area of the arterial lumen if no plaque had been present. The internal elastic lamina area correlated directly with the area of the lesion (r = 0.44, P less than 0.001), suggesting that coronary arteries enlarge as lesion area increases. Regression analysis yielded the following equation: Internal elastic lamina area = 9.26 + 0.88 (lesion area) + 0.026 (age) + 0.005 (heart weight). The correlation coefficient for the lesion area was significant (P less than 0.001), whereas the correlation coefficients for age and heart weight were not. The lumen area did not decrease in relation to the percentage of stenosis (lesion area/internal elastic lamina area X 100) for values between zero and 40 percent but did diminish markedly and in close relation to the percentage of stenosis for values above 40 percent (r = -0.73, P less than 0.001). We conclude that human coronary arteries enlarge in relation to plaque area and that functionally important lumen stenosis may be delayed until the lesion occupies 40 percent of the internal elastic lamina area. The preservation of a nearly normal lumen cross-sectional area despite the presence of a large plaque should be taken into account in evaluating atherosclerotic disease with use of coronary angiography.
High frequency performance of multilayer ceramic capacitors (2002)

The monolithic ceramic capacitor (MLC) is small and has good high frequency performance. It is used in various high frequency circuits for impedance matching, DC block, filter and bypass functions. For the best performance in these applications, low equivalent series resistance (ESR) and equivalent series inductance (ESL) are required. We have tested the performance of several MLC designs to find out the effect of the ESR and ESL. We have developed an ultra small capacitor that has good performance for high frequency and high speed digital circuits. In order to measure the high frequency performance accurately, we have developed a test fixture using a microstrip design. The fixture has a return loss of greater than 30 dB and an insertion loss less than 0.5 dB. The MLC is mounted on the microstrip fixture and the return loss and insertion loss are measured. A network analyzer is used to compensate for the fixture to obtain good data up to 15 GHz.
Programmed Assembly of DNA Functionalized Quantum Dots (1999)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTProgrammed Assembly of DNA Functionalized Quantum DotsGregory P. Mitchell, Chad A. Mirkin, and Robert L. LetsingerView Author Information Department of Chemistry, Northwestern University 2145 Sheridan Rd., Evanston, Illinois 60208 Cite this: J. Am. Chem. Soc. 1999, 121, 35, 8122–8123Publication Date (Web):August 18, 1999Publication History Received19 May 1999Published online18 August 1999Published inissue 1 September 1999https://pubs.acs.org/doi/10.1021/ja991662vhttps://doi.org/10.1021/ja991662vrapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views6937Altmetric-Citations595LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Chemical structure,Genetics,Metal nanoparticles,Nanoparticles,Quantum dots Get e-Alerts
A method for detection of randomly placed objects for robotic handling (2016)

The image based systems still have open issues in order to meet the latest manufacturing requirements for simplicity, low cost as well as the limited maintenance requirements. In this direction, there is a method proposed for the recognition of 3D randomly placed objects for eventual robotic handling. The method includes a 2D vision system and is combined with data from computer-aided design (CAD) files for the generation of 3D coordinates. It is generic and can be used for the identification of multiple randomly placed objects. The method has been implemented in a software tool using MATLAB® and has been applied to a consumer goods case for the recognition of shaver handles.
Recent advances in the concept of hard and soft acids and bases (1987)

The hard / soft acids / bases principle has been justifiably criticized because of the lack of a precise definition of hardness and the inability to quantify this property; recent developments have overcome these objections, however.
Identification of novel neuroendocrine-specific tumour genes (2008)

Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling, sought to identify genes that are either up- or downregulated in NE as compared with non-NE tumour cells. A panel of six NET and four non-NET cell lines were examined, and out of 12 743 genes examined, we studied in detail the 200 most significantly differentially expressed genes in the comparison. In addition to potential new diagnostic markers (NEFM, CLDN4, PEROX2), the results point to genes that may be involved in the tumorigenesis (BEX1, TMEPAI, FOSL1, RAB32), and in the processes of invasion, progression and metastasis (MME, STAT3, DCBLD2) of NETs. Verification by real time qRT–PCR showed a high degree of consistency to the microarray results. Furthermore, the protein expression of some of the genes were examined. The results of our study has opened a window to new areas of research, by uncovering new candidate genes and proteins to be further investigated in the search for new prognostic, predictive, and therapeutic markers in NETs.