gebhart/patrepeval huggingface dataset:
ZD1839 (IRESSA), an EGFR‐selective tyrosine kinase inhibitor, enhances taxane activity in bcl‐2 overexpressing, multidrug‐resistant MCF‐7 ADR human breast cancer cells (2002)

Constitutive bcl-2 overexpression increases the tumorigenic and metastatic potential of doxorubicin-resistant, estrogen-independent, MCF-7 ADR human breast cancer cells. We evaluated the sensitivity to taxanes (paclitaxel, docetaxel and IDN 5109) of 2 bcl-2-overexpressing MCF-7 ADR clones and control neomycin-transfected MCF-7 ADR neo cells. The 2 bcl-2-overexpressing MCF-7 ADR clones were relatively resistant to all 3 taxanes, whereas the MCF-7 ADR neo cells were relatively resistant to paclitaxel and docetaxel, but sensitive to IDN 5109. We found that both MCF-7 ADR neo and bcl-2-overexpressing MCF-7 ADR clones express high levels of the epidermal growth factor receptor (EGFR) and its ligand, transforming growth factor-alpha (TGF-alpha). Therefore, we tested the growth inhibitory effect of ZD1839 (Iressa, AstraZeneca, Macclesfield, UK), an orally active, selective EGFR tyrosine kinase inhibitor (EGFR-TKI) that is in clinical development. ZD1839 inhibited the growth in soft agar of all 3 clones in a dose-dependent manner (IC(50) of approximately 0.1 microm). This effect was accompanied by a dose-dependent inhibition of EGFR tyrosine autophosphorylation and of the production of TGF-alpha, basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). To determine whether the blockade of EGFR signaling might affect the sensitivity of bcl-2-overexpressing MCF-7 ADR cells to taxanes, cells were treated with ZD1839 in combination with paclitaxel, docetaxel or IDN 5109, and dose-dependent cooperative growth inhibition as well as apoptosis potentiation were observed. Combined treatment with IDN 5109 and ZD1839 also resulted in a significant inhibition of bcl-2 expression in bcl-2-overexpressing MCF-7 ADR cells. These results demonstrate the ability of ZD1839 to overcome taxane resistance in a model of hormone-independent, multidrug-resistant, human breast cancer.
Expression pattern of M‐cadherin in normal, denervated, and regenerating mouse muscles (1994)

Following muscle damage in adult vertebrates, myofibers can be regenerated from muscle precursor cells (satellite cells). During this process, prenatal myogenesis is recapitulated to a large extent, both morphologically and molecularly. A putative morphoregulatory molecule involved in myogenesis is M-cadherin (Mcad), a calcium-dependent cell adhesion protein. The expression of Mcad was studied by immunofluorescence in regenerating, denervated, and normal mouse muscles. Our results demonstrate that Mcad is present in satellite cells in normal muscle. Enhanced staining at sites of contact between satellite cells and the parent muscle fiber suggests an additional, spatially restricted expression of Mcad in muscle fibers. Mcad positive cells in normal and denervated muscles did not incorporate bromodeoxyuridine within 24 hr after injection in vivo, indicating that Mcad is expressed on mitotically quiescent satellite cells. Neural cell adhesion molecule (NCAM) co-localized with Mcad in nearly all satellite cells in denervated muscles but rarely in intact muscles. At early stages of regeneration, Mcad was exclusively and strongly expressed in myoblasts. After fusion of myoblasts into myotubes, Mcad was down-regulated and was barely detectable on more mature myotubes surrounded by distinct basal lamina sheaths. These observations are in line with the idea that Mcad plays a crucial role in myogenesis. In intact muscle, Mcad might function as a molecular link between satellite cell and muscle fiber.
Synthesis and sintering of ZrNiSn thermoelectric compounds (2003)

In contrast to the commonly used arc melting method, samples in the present paper were prepared by the solid-state reaction from element powders at 1173K under a flowing argon atmosphere for 4-7 days. The constituent phases and the element compositions were determined and showing that samples were of single phase and stoichiometric. ZrNiSn powder was then consolidated by using the spark plasma sintering technique. The effect of sintering temperature and holding time on the density was discussed. It is found that, dense ZrNiSn compounds with fine grain size and homogeneous microstructure were achieved under the condition of 1123K-40MPa-25min.
Effects of single-dose interleukin-12 exposure on interleukin-12-associated toxicity and interferon-gamma production. (1997)

Interleukin-12 (IL-12) is a key regulator of cell-mediated immunity that has therapeutic potential in cancer and infectious disease. In a previous Phase 1 dose escalation study of a single test dose of recombinant human IL-12 (rhIL-12) followed 14 days later by cycles of five consecutive daily intravenous injections every 3 weeks, we showed that a dose level up to 500 ng/kg could be administered with acceptable levels of safety. Based on these results, a Phase 2 study was conducted. In the Phase 2 study, however, administration of rhIL-12 at this same dose level resulted in severe toxicities with some patients unable to tolerate more than two successive doses. Of the 17 patients receiving rhIL-12 in the Phase 2 study, 12 patients were hospitalized and two patients died. A thorough scientific investigation to determine the cause of this unexpected toxicity failed to identify any difference in the drug products used or the patient populations enrolled in the Phase 1 and Phase 2 studies that could have accounted for the profound difference in toxicity. The focus of the investigation therefore shifted to the schedule of rhIL-12 administration. We determined that a single injection of rhIL-12 2 weeks before consecutive dosing included in the Phase 1 study, but not in the schedule of administration in the Phase 2 study, has a profound abrogating effect on IL-12-induced interferon-gamma (IFN-gamma) production and toxicity. This observation of schedule-dependent toxicity of IL-12 has been verified in mice, as well as nonhuman primates. In this regard, a single injection of IL-12 before consecutive daily dosing protected mice and cynomolgus monkeys from acute toxicity including mortality and was associated with an attenuated IFN-gamma response. Because of this unique biologic response, careful attention to the schedule of administration is required to assure safe and effective clinical development of this highly promising cytokine.
Gender classification from infants to seniors (2010)

Many believe that gender classification is a solved problem, however, gender classification for children is a very difficult problem that has not been adequately addressed by the research community. In this work we demonstrate this fact and present a system that performs gender classification on children that outperforms humans. Motivated by the significant improvement in model selection for age estimation, we investigate a robust gender classification system via model selection and evaluate the systems using leave-one-person-out cross-validation and 5-fold cross-validation schemes on FG-NET database. Furthermore, this work develops a novel operator, graph gender preserving, to build a neighborhood graph for locality preserving projection for gender classification.
Rabbit aortic smooth muscle cells express inducible macrophage scavenger receptor messenger RNA that is absent from endothelial cells. (1992)

Scavenger receptors mediate uptake of modified low density lipoproteins by macrophages. The accumulation of lipids via this process is thought to lead to foam cell formation in developing atherosclerotic plaques. Smooth muscle cells, which can also be converted to foam cells in vivo, have not been shown to express the same scavenger receptor previously cloned in macrophages. We report the cloning of two cDNAs that encode type I and type II scavenger receptors isolated from rabbit smooth muscle cells. The deduced protein sequences of these isolates are highly homologous to the scavenger receptors previously isolated from macrophages. Treatment of smooth muscle cells with phorbol esters induced a marked increase in scavenger receptor mRNA and a fivefold increase in receptor degradation activity. Rabbit venous endothelial cells in primary culture and a bovine aortic endothelial cell line had no detectable scavenger receptor mRNA, despite having scavenger receptor degradation activity. The latter finding suggests that endothelial cells may possess a scavenger receptor which is structurally distinct from that found in macrophages and smooth muscle cells. The isolation of cDNAs encoding the rabbit scavenger receptor should prove useful for in vitro and in vivo studies that employ the rabbit as a model of human atherosclerosis.
Involvement of spinal calcitonin gene‐related peptide in the development of acute visceral hyperalgesia in the rat (2001)

This study aimed to characterize the role of the neuropeptide calcitonin gene-related peptide (CGRP) in the development of mechanically induced visceral hyperalgesia. Tonic colorectal distension (CRD) was performed in fasted, conscious male Sprague-Dawley rats. The visceromotor reflex associated with noxious CRD was determined as the number of contractions during each of two consecutive tonic distensions (10 min at 60 mmHg), which were separated by a series of phasic distensions (repeated 15-s distensions to 80 mmHg at 30-s intervals). The effect of the CGRP receptor antagonist h-CGRP8-37 given intrathecally (i.t.) (0.03-3 nmol rat-1) or intravenously (i.v.) (20 microg kg-1 bodyweight [bw]) on the visceromotor response was evaluated. The dose for i.v. administration was chosen based on previous results from similar studies. In addition, the effect of a CGRP monoclonal antibody (6 mg kg-1 bw) given intravenously was evaluated. Compared to the baseline response, a significant increase in the number of abdominal contractions was observed during the second tonic distension. The i.t. application of h-CGRP8-37 dose-dependently reduced the numbers of abdominal contractions both during the first and the second tonic distension period, with a maximum effect observed at a peptide concentration of 3 nmol. Intravenous administration of h-CGRP8-37 or of the CGRP antiserum produced a small reduction of the visceromotor response induced by the second tonic distension and had no effect on colonic compliance. The development of mechanically induced colorectal hyperalgesia by repeated tonic distension involves the spinal release of CGRP, while peripheral release of CGRP plays only a minor role.
Una contribución a la educación y orientación de la sexualidad, en los adolescentes (2011)

Se realizó un análisis bibliográfico de la literatura vinculada a la educación sexual para profundizar en los aspectos generales e importantes del tema. Se procede a la realización de un diagnóstico inicial en una población de 30 estudiantes del 11no grado del municipio Amancio, Según las dificultades detectadas en el diagnóstico se elaboraron actividades dirigidas a darle solución a las mismas. Con este proyecto de actividades se pretende que los estudiantes se apropien de conocimientos sobre aspectos relacionados con la educación sexual, al culminar se evidencian cambios en el modo de actuación en la mayoría de los adolescentes.
Purification of recombinant human prostromelysin. Studies on heat activation to give high-Mr and low-Mr active forms, and a comparison of recombinant with natural stromelysin activities (1991)

Recombinant human prostromelysin was purified in a single step using Procion Red-Sepharose chromatography. The purified prostromelysin was self-activated to high-Mr (45,000) and low-Mr (28,000) forms by incubation at 55 degrees C without the addition of extraneous activators. The two forms of stromelysin were subsequently separated, again using Procion Red-Sepharose. Both of the heat-activated recombinant forms demonstrated similar specific activities (for the macromolecular substrates casein, gelatin, elastin, proteoglycan and type IV collagen) when compared with either heat- or trypsin-activated natural stromelysin. The heat-activated recombinant stromelysins both showed similar abilities to potentiate activation of human procollagenase when compared with trypsin-activated natural stromelysin.
The Relationship Between Vaccine Dose and Efficacy in Channel Catfish Ictalurus punctatus Vaccinated as Fry with a Live Attenuated Strain of Edwardsiella ictaluri (RE-33) (2001)

Channel catfish Ictalurus punctatus were vaccinated at 12 d of age (post-hatch) by a 2-min bath immersion with attenuated Edwardsiella ictaluri RE-33 at doses of 2.5 × 105, 2.5 × 106, and 2.4 × 107 colony-forming units CFU/mL of water. Following vaccination, RE-33 was recovered from a greater percentage of fry that were vaccinated at the high and intermediate doses compared to fry vaccinated at the lowest dose. Independent of dose, the greatest percentage of RE-33 positive fry occurred between 1 and 6 d post-vaccination with a significant decrease in positive fry observed on day 12. A significant increase in mortality occurred 6 to 12 d post-vaccination in fry vaccinated at the highest dose. No differences in post-vaccination mortalities occurred between the other treatments. Following virulent E. ictaluri challenge, mortalities of fish vaccinated at doses of 2.5 × 106 and 2.4 × 107 CFU/mL were significantly less than those of fish vaccinated at 2.5 × 105 CFU/mL and sham-vaccinated control fish. These data show that vaccination with RE-33 can offer protection against subsequent virulent E. ictaluri infection.
Reappraisal and neotypification of Phyllachora feijoae (2012)

Acca sellowiana (Myrtaceae), feijoa (in Brazil, goiaba da serra), is a native southern South America tree that produces edible fruits which, although only occasionally cultivated in South America, became a significant fruit crop in New Zealand. Recently, during surveys for fungal pathogens of feijoa in southern Brazil, several plants were found bearing tar-spot symptoms caused by a species of Phyllachora. A literature search enabled us to identify the fungus as Phyllachora feijoae, a little-known species originally described in the 19(th) century by H. Rehm and later transferred to the genus Catacauma. The name Catacauma feijoae, although now regarded as a later synonym of P. feijoae is still mistakenly in use (as, for instance, in the Brazilian list of fungi on plants). The type specimen was most probably deposited in the Botanisches Garten und Museum Berlin-Dahlem (B) and lost or destroyed during World War II, and could not be located. The recent recollection of abundant material of this fungus in the vicinity of Pelotas (Rio Grande do Sul, Brazil) allowed its re-examination and neotypification. Phyllachora feijoae is also illustrated here for the first time.
Dendritic cell ontogeny: A human dendritic cell lineage of myeloid origin (1997)

Dendritic cells (DC) have been thought to represent a family of closely related cells with similar functions and developmental pathways. The best-characterized precursors are the epidermal Langerhans cells, which migrate to lymphoid organs and become activated DC in response to inflammatory stimuli. Here, we demonstrate that a large subset of DC in the T cell-dependent areas of human lymphoid organs are nonactivated cells and belong to a separate lineage that can be identified by high levels of the interleukin 3 receptor alpha chain (IL-3Ralphahi). The CD34+IL-3Ralphahi DC progenitors are of myeloid origin and are distinct from those that give rise to Langerhans cells in vitro. The IL-3Ralphahi DC furthermore appear to migrate to lymphoid organs independently of inflammatory stimuli or foreign antigens. Thus, DC are heterogeneous with regard to function and ontogeny.
Towards a new generation of analogue IC design architectures (1990)

More mature device technologies such as true complementary silicon bipolar junction (BJT), mixed silicon bipolar and complimentary metal oxide semiconductor devices (BiCMOS) and Gallium Arsenide (GaAs) are becoming available and bring with them the requirement for novel analogue design, methods, techniques and CAD tools necessary for the successful development and exploitation of these technologies for the future market place. Coupled with these technological improvements are the ever shrinking feature size of devices on ICs and the consequential reduction of power supply voltages which has fuelled the creation of 'alternative' analogue design methods. This paper is a review of some of the authors' work on the design and application of current-mode analogue signal processing techniques and the development of a new generation of high performance analogue circuit and system architectures, with emphasis on high speed.<
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Predictive Modeling in a VoIP System (2013)

An important problem one needs to deal with in a Voice over IP system is server overload. One way for preventing such problems is to rely on prediction techniques for the incoming traffic, namely as to proactively scale the available resources. Anticipating the computational load induced on processors by incoming requests can be used to optimize load distribution and resource allocation. In this study, the authors look at how the user profiles, peak hours or call patterns are shaped for a real system and, in a second step, at constructing a model that is capable of predicting trends.
Scalable Manufacture of Built‐to‐Order Nanomedicine: Spray‐Assisted Layer‐by‐Layer Functionalization of PRINT Nanoparticles (2013)

Scalable methods, PRINT particle fabrication, and spray-assisted Layer-by-Layer deposition are combined to generate uniform and functional nanotechnologies with precise control over composition, size, shape, and surface functionality. A modular and tunable approach towards design of built-to-order nanoparticle systems, spray coating on PRINT particles is demonstrated to achieve technologies capable of targeted interactions with cancer cells for applications in drug delivery.
Tenuecyclamides A−D, Cyclic Hexapeptides from the Cyanobacterium Nostoc spongiaeforme var. tenue (1998)

Four modified cyclic hexapeptides, tenuecyclamides A-D (1-4), were isolated along with the known antibiotic, borophycin (5), from the methanol extract of Nostoc spongiaeforme var. tenue (TAU strain IL-184-6). The planar structure of tenuecyclamides A-D (1-4) was determined by homonuclear and inverse-heteronuclear 2D-NMR techniques as well as by high-resolution mass spectrometry measurements. The absolute configuration of the asymmetric centers was studied by Marfey's method for HPLC. The stereochemistry of the asymmetric centers in tenuecyclamides A and B (1 and 2) could not be fully determined, while that of tenuecyclamides C and D (3 and 4) was unambiguously determined.
Sorbents for the Direct Capture of CO2 from Ambient Air (2019)

The urgency to address global climate change induced by greenhouse gas emissions is increasing. In particular, the rise in atmospheric CO<sub>2</sub> levels is generating alarm. Technologies to remove CO<sub>2</sub> from ambient air, or "direct air capture" (DAC), have recently demonstrated that they can contribute to "negative carbon emission." Recent advances in surface chemistry and material synthesis have resulted in new generations of CO<sub>2</sub> sorbents, which may drive the future of DAC and its large-scale deployment. This Review describes major types of sorbents designed to capture CO<sub>2</sub> from ambient air and they are categorized by the sorption mechanism: physisorption, chemisorption, and moisture-swing sorption.
Digital materials for digital fabrication (2007)

This thesis introduces digital materials by analogy with digital computation and digital communications. Traditional fabrication techniques include pick-and-place, roll-to-roll, molding, patterning and more. Current research in fabrication includes algorithmic assembly [3], programmed assembly[9], self-assembly[1,2], assembly by folding [4] as well as guided self-assembly [2]. While these research areas are studying means of fabrication, here we introduce the study of the digital materials they assemble. Moreover we present a new type of three-dimensional digital printer for use with functional digital materials. Most importantly, the digital materials are shown to be tuneable; the code describing a digital material allows one to predict and adjust the properties of the material itself. In the same way digital communications and computation are discrete in the code space, digital fabrication is discrete in the physical space. Just as digital communications enabled cheap long-distance communications and digital computation enabled cheap,universal and efficient computers, digital fabrication enables cheap, efficient and
Optical pattern recognition (1992)

We discuss how one optical processor (a correlator) can be used for all levels of scene analysis (low, medium, and high-level computer vision). This is achieved by the use of different filter functions for the different levels of a hierarchical inference system. New optical processor filter research that allows such flexibility is advanced and examples of each of these filters are provided. For large class problems, feature extracted from each region of interest in a scene are fed to a neural net processor which performs recognition. New algorithms for optical neural net classifiers are also required. We conclude that present hardware optical correlator advances can significantly benefit from such processing.
Molecular determinants for activation and inactivation of HERG, a human inward rectifier potassium channel. (1996)

1. The human eag-related potassium channel, HERG, gives rise to inwardly rectifying K+ currents when expressed in Xenopus oocytes. 2. The apparent inward rectification is caused by rapid inactivation. In extracellular Cs+ solutions, large outward currents can be recorded having an inactivation time constant at 0 mV of about 50 ms with an e-fold change every 37 mV. 3. HERG channel inactivation is not caused by an amino-terminal ball structure, as a deletion of the cytoplasmic amino terminus (HERG delta 2-373) did not eliminate inactivation. However, channel deactivation was accelerated about 12-fold at -80 mV. 4. Mutation of S631 to A, the homologous residue of eag channels, in the outer mouth of the HERG pore completely abolished channel inactivation. 5. Activity of HERG channels depended on extracellular cations, which are effective for channel activation, in the order Cs+ > K+ > > Li+ > Na+. The point mutation S631A strongly reduced this channel regulation. 6. By analogy to functional aspects of cloned voltage-gated potassium channels, rectification of HERG, as well as its kinetic properties during the course of an action potential, are presumably governed by a mechanism reminiscent of C-type inactivation.