gebhart/patrepeval huggingface dataset:
Inhibition of tumor cell growth by monoterpenes in vitro: evidence of a Ras-independent mechanism of action (1996)

(+)-Limonene (d-limonene) and related monoterpenes show chemopreventive activity against rodent mammary carcinoma and inhibit the growth of cancer cells in vitro. One suggested mechanism for the anti-tumorigenic effect of (+)-limonene is inhibition of the post-translational isoprenylation of growth controlling Ras oncoproteins. We have here examined the growth inhibitory effect of (+)-limonene and other related monoterpenes on PANC-1 pancreas carcinoma cells (carrying a K-ras mutation) and on 12V-H-ras-transformed rat fibroblasts. (+)- and (-)-perillyl alcohol, 7-methyl-perillyl alcohol, (+)-limonene oxide and (+)-perillic acid methyl ester were all found to efficiently inhibit cell growth at 1 mM, whereas (+)-limonene caused an approximately 50% growth reduction at 5 mM. Whereas BZA-5B, an inhibitor of Ras farnesyl transferase, was found to induce morphological reversion of 12V-H-ras-transformed cells, (+)-perillyl alcohol and (+)-limonene did not induce reversion. Furthermore, monoterpenes did not decrease MAP kinase enzyme activity or collagenase promoter activity in PANC-1 cells, two functions known to be down-stream from Ras. We conclude that although effective in inhibiting the growth of tumor cells harboring activated ras oncogenes, limonene and (+)-perillyl alcohol are unlikely to act by inhibiting Ras function.
myc and E1A oncogenes alter the responses of PC12 cells to nerve growth factor and block differentiation. (1987)

PC12 rat pheochromocytoma cells respond to nerve growth factor (NGF) by neuronal differentiation and partial growth arrest. Mouse c-myc and adenovirus E1A genes were introduced into PC12 cells to study the influence of these nuclear oncogenes on neuronal differentiation. Expression of myc or E1A blocked morphological differentiation and caused NGF to stimulate rather than inhibit cell proliferation. NGF binding to cell surface receptors and ornithine decarboxylase induction were similar in myc- and E1A-expressing clones compared with wild-type PC12 cells, suggesting that changes in the cellular response to NGF were at a post-receptor level. These results illustrate that NGF can promote either growth or differentiation of PC12 cells, and that myc or E1A alter the phenotypic responses to growth factors and hormones.
Depth-resolved displacement measurement using tilt scanning speckle interferometry (2006)

We describe a novel technique that we call Tilt Scanning Interferometry (TSI) to measure depth-resolved structure and displacement fields within semitransparent scattering materials. The method differs significantly from conventional optical coherence tomography in that only one wavelength is used throughout the whole measurement process. Temporal sequences of speckle interferograms are recorded whilst the illumination angle is tilted at a constant rate. Fourier transformation of the resulting three-dimensional (3-D) intensity distribution along the time axis reconstructs the scattering potential within the medium. Repeating the measurements with the object wave at equal and opposite angles about the observation direction results in two 3-D phase-change volumes, the sum of which gives the out-of-plane-sensitive phase volume and the difference between which gives the in-plane phase volume. From these phase-change volumes the in-plane and out-of-plane depth-resolved displacement fields are obtained. The theoretical framework for the technique is outlined and results from proof-of-principle experiments involving a semitransparent beam undergoing bending are presented.
Structure-function relationships in the Ca(2+)-binding and translocation domain of SERCA1: physiological correlates in Brody disease. (1998)

Alanine-scanning mutagenesis of all amino acids in transmembrane helices M4, M5, M6 and M8, which contain known Ca2+ binding residues in the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum, revealed patches of mutation-sensitivity in M4, M5 and M6, but in M8. A six-residue motif, (E/D)GLPA(T/V), in M4 and M6 and its counterpart in M5 were highlighted by mutagenesis. Site-directed disulfide mapping of helices M4 and M6 demonstrated that these transmembrane helices associate as a right-handed coiled-coil. This structural information, combined with the earlier analysis of the association of each Ca2+ binding residue with either Ca2+ binding site I or site II, permitted the development of a "side-by-side" model for the two Ca2+ binding sites in the Ca(2+)-ATPase. In about half of Brody disease families, mutations create stop codons which delete all or part of the Ca2+ binding and translocation domain, resulting in loss of SERCA1 function and muscle disease.
Synthesis and Biological Evaluation of Aza-Epothilones (2000)

A macrolactam scaffold rather than the natural macrolactone scaffold is the characteristical feature of a new class of epothilone A analogues (see picture). These aza-epothilones were synthesized with a modular approach in pure form and excellent yields, and their biological properties were evaluated.
Geographical averaging and ancillary services for stochastic power generation (2010)

The distribution of stochastic generation from renewables across different geographical locations can, in certain cases, help to mitigate the inherent variability in output. This variability of generation from renewables may 1) increase the operating costs of the conventional generators used to follow the net load not supplied by stochastic capacity and 2) increase the amount of reserve conventional generating capacity needed to maintain Operating Reliability. In this scenario, customers have lower wholesale prices, due to reductions in the total annual generation from fossil fuels, while generators face higher operating costs for conventional generators caused by additional ramping that partly offset the customer benefits. However, the lower wholesale prices ($/MWh) imply lower annual earnings for conventional generators that lead to higher amounts of missing money ($/MW) needed to maintain the financial adequacy of installed generating units. The objective of this paper is to determine how variability from a stochastic generation resource affects the optimal hour-to-hour dispatch of generating units and the corresponding operating costs and wholesale prices. The results show that the inclusion of ramping costs for conventional generation affect the amount of energy dispatched from the stochastic generator, and the total costs composition observed in the system. The Cornell SuperOPF1 is used to illustrate how the operating costs and wholesale prices can be determined for a reliable network (the amount of conventional generating capacity needed to maintain Operating Reliability is determined endogenously). The results in this paper use a typical daily pattern of load and capture the cost of ramping by including additions to the operating costs of the generating units associated with the hour-to-hour changes in their optimal dispatch. The calculations for determining endogenous up and down reserves are included, and the wind generation cost is assumed to be zero. Additionally, the maximum and minimum available capacities for all hours in the day are constrained to the optimal capacities for the hours with the highest and the lowest loads. Different scenarios are evaluated for a given hourly realization of wind speeds using specified amounts of installed wind capacity with and without ramping costs. The analysis also evaluates the effects of eliminating network constraints, as well as the elimination of wind variability by accounting for the effects of spatial aggregation of different wind locations.
Influence of low direct electric currents and chlorhexidine upon human dental biofilms (2016)

Dental biofilms have been widely associated with biological complications of oral implants. Currently, no consensus exists regarding the most reliable anti-infective approach to treat peri-implantitis. This study aimed to investigate whether low direct electric currents (DC) could influence chlorhexidine (CHX) 0.2% antimicrobial efficacy against human dental biofilms. To support biofilm accumulation, discs made with machined titanium (Ti) or hydroxyapatite (HA) were used. Five volunteers wore during 24 h an intraoral thermoformed splint on which ten specimens were bonded. Biofilms were then collected and treated ex vivo. During each antimicrobial experiment (<i>N</i> = 20 replicates), two modalities of treatment (CHX/PBS = control groups and CHX/PBS+5mA = test groups) were tested (<i>n</i> = 5 discs each) and the number of viable bacteria evaluated in LogCFU/mL at baseline, 0.5, 1, 2 and 5 min. The proportion of killed bacteria was also estimated and compared statistically at each time point between control and test groups. CHX+/-5mA induced a mean viability reduction around 90-95% after 5 min of treatment whatever the surface considered (Ti/HA). A significant difference regarding the bactericidal effect was noted on Ti surfaces after 0.5, 1 and 2 min in favor of the CHX+5mA modality when compared to CHX alone (<i>p</i> < 0.05). PBS+5mA also had a certain antimicrobial effect (58%) after 5 min on Ti surfaces. This effect was significantly higher than that observed with PBS (25%) (<i>p</i> < 0.05). This study showed that low DC (5mA) can have an antibiofilm effect and are also able to enhance chlorhexidine 0.2% efficacy against human dental biofilms grown on titanium surfaces.
Determination of monoisotopic masses and ion populations for large biomolecules from resolved isotopic distributions (1995)

The coupling of electrospray ionization with Fourier-transform mass spectrometry allows the analysis of large biomolecules with mass-measuring errors of less than 1 ppm. The large number of atoms incorporated in these molecules results in a low probability for the all-monoisotopic species. This produces the potential to misassign the number of heavy isotopes in a specific peak and make a mass error of ±1 Da, although the certainty of the measurement beyond the decimal place is greater than 0.1 Da. Statistical tests are used to compare the measured isotopic distribution with the distribution for a model molecule of the same average molecular mass, which allows the assignment of the monoisotopic mass, even in cases where the monoisotopic peak is absent from the spectrum. The statistical test produces error levels that are inversely proportional to the number of molecules in a distribution, which allows an estimation of the number of ions in the trapped ion cell. It has been determined, via this method that 128 charges are required to produce a signal-to-noise ratio of 3:1, which correlates well with previous experimental methods.
Comparative efficacy and safety of 3-day azithromycin and 10-day penicillin V treatment of group A beta-hemolytic streptococcal pharyngitis in children (1996)

The efficacy and safety of a 3-day course of azithromycin oral suspension (10 mg/kg of body weight once daily) were compared with those of penicillin V (50,000 U/kg/day in two divided doses) in children aged 3 to 12 years for the treatment of symptomatic pharyngitis caused by the group A beta-hemolytic streptococcus (GABHS). For the 154 evaluable patients, the original infecting strain of GABHS was eliminated at the end of follow-up (34 to 36 days after treatment started) from 67 (85.8%) of 78 penicillin-treated patients and 41 (53.9%) of 76 azithromycin-treated patients (P < 0.0001). Overall clinical success was achieved in 71 (91.0%) of 78 penicillin V-treated patients and 57 (75.0%) of 76 azithromycin-treated patients (P < 0.05). Potential drug-related adverse events were reported for 5.5 and 8.6% of the penicillin V- and azithromycin-treated patients, respectively (P = 0.6). In the present study, a once-daily (10 mg/kg), 3-day oral regimen of azithromycin was as safe as a 10-day course of penicillin but did not represent an effective alternative to penicillin for the treatment of GABHS pharyngitis, even for those children with azithromycin-susceptible strains.
Long-working-distance synthetic aperture Fresnel off-axis digital holography (2009)

An aperture synthesis approach of digital holography for microscopy imaging at long working distance is proposed. Firstly, for an oblique object, a series of Fresnel off-axis holograms are recorded with different tilted plane wave illuminations without using lens for pre-magnification. Then the complex amplitudes are reconstructed and magnified from these holograms by the double-step Fresnel reconstruction method respectively. Finally, the synthesized image of the resolution enhanced and the speckle suppressed is obtained by incoherent superposition of these reconstructed complex amplitudes. The important advantage of the proposed approach is that the working distance of the system isn't constrained and the reconstructed image doesn't subject to lens aberrations. The experimental results with a die and an USAF-1951 resolution test target are shown and demonstrated that the resolution of both intensity and phase image can be effectively enhanced with simple set-up and procedure. The proposed approach can improve the capabilities of digital holography in three-dimensional in-situ microscopy at long working distance.
Modeling and analysis of non-rectangular transistors caused by lithographic distortions (2008)

Sub-wavelength lithography causes the shape of transistors to differ from idealized rectangles. Several researchers have proposed transistor simulation models to characterize the non-ideal shapes of transistors for various regions of transistor operation. It has been shown that the effective channel length of a non-rectangular gate (NRG) transistor may be different for ON and OFF currents. In this paper we present a composite post-litho non-rectangular transistor model that not only accounts for DC behavior of a transistor, but also accounts for parasitic capacitances across a range of voltages. Parameters of this model can be fitted to real silicon data. The proposed model has been validated by TCAD device simulations. Results show that a composite model that accounts for both DC currents and parasitic capacitances is no more complex than models optimized for DC currents only. Further, the proposed model integrates readily with available SPICE simulators. We have also presented cell library characterization data to illustrate the benefit of using a delay accurate transistor model.
Deg phenotype of Escherichia coli lon mutants (1978)

Deg. one of the Escherichia coli systems for degrading abnormal polypeptides (e.g., nonsense fragments), is also involved in the degradation of some classes of missense proteins. Both missense proteins of beta-galactosidase and temperature-sensitive phage products appear to be degraded by the Deg system. Mutations in the Deg system are indistinguishable from mutations classically called lon or capR; all map near proC, all are mucoid, defective in protein degradation, sensitive to radiomimetic agents, and defective in P1 lysogenization. All are able to propagate temperature-sensitive phage better than lon+ parental strains. Mutations that suppress the radiation sensitivity of these strains (sul) also suppress the P1 lysogenization defect, but do not affect mucoidy or the degradation defect.
Macrophage Migration Inhibitory Factor Induces Macrophage Recruitment via CC Chemokine Ligand 2 (2006)

Macrophage migration inhibitory factor (MIF) was originally identified for its ability to inhibit the random migration of macrophages in vitro. MIF is now recognized as an important mediator in a range of inflammatory disorders. We recently observed that the absence of MIF is associated with a reduction in leukocyte-endothelial cell interactions induced by a range of inflammatory mediators, suggesting that one mechanism whereby MIF acts during inflammatory responses is by promoting leukocyte recruitment. However, it is unknown whether MIF is capable of inducing leukocyte recruitment independently of additional inflammatory stimuli. In this study, we report that MIF is capable of inducing leukocyte adhesion and transmigration in postcapillary venules in vivo. Moreover, leukocytes recruited in response to MIF were predominantly CD68(+) cells of the monocyte/macrophage lineage. Abs against the monocyte-selective chemokine CCL2 (JE/MCP-1) and its receptor CCR2, but not CCL3 and CXCL2, significantly inhibited MIF-induced monocyte adhesion and transmigration. CCL2(-/-) mice displayed a similar reduction in MIF-induced recruitment indicating a critical role of CCL2 in the MIF-induced response. This hypothesis was supported by findings that MIF induced CCL2 release from primary microvascular endothelial cells. These data demonstrate a previously unrecognized function of this pleiotropic cytokine: induction of monocyte migration into tissues. This function may be critical to the ability of MIF to promote diseases such as atherosclerosis and rheumatoid arthritis, in which macrophages are key participants.
Development of high accuracy phase control method for space solar power system (2011)

SSPS (Space Solar Power System) consists of many phased array antenna panels in space. The panels are large and light weight, so the panels are flexible structure. Therefore, there is a possibility to emit unexpected microwaves. To resolve the problem, MHI (Mitsubishi Heavy Industries, Ltd) has developed two types of high accuracy phase control methods. The first is `PAC (Position and Angle Correction) method'. The second is `Parallel method'. Prototype systems of the two methods were developed. By the methods, the phase deviation was controlled within less than 1 degree accuracy.
Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) by host cells (2005)

Clostridium difficile toxin B (TcdB) is a single-stranded protein consisting of a C-terminal domain responsible for binding to the host cell membrane, a middle part involved in internalization, and the N-terminal catalytic (toxic) part. This study shows that TcdB is processed by a single proteolytic step which cleaves TcdB(10463) between Leu(543) and Gly(544) and the naturally occurring variant TcdB(8864) between Leu(544) and Gly(545). The cleavage occurs at neutral pH and is catalysed by a pepstatin-sensitive protease localized in the cytoplasm and on the cytoplasmic face of intracellular membranes. The smaller N-terminal cleavage products [63 121 Da (TcdB(10463)) and 62 761 Da (TcdB(8864))] harbour the cytotoxic and glucosyltransferase activities of the toxins. When microinjected into cultured Chinese hamster lung fibroblasts, the N-terminal cleavage fragment shows full cytotoxic activity shortly after injection whereas the holotoxin initially exhibits a very low activity which, however, increases with time. Twenty minutes after the start of internalization of TcdB, the larger cleavage products [206 609 Da (TcdB(10463)) and 206 245 Da (TcdB(8864))] are found exclusively in a membrane fraction, whereas the N-terminal cleavage products appear mainly in the cytosol and associated with the membrane. This is in line with a proposed model according to which the longer, C-terminal, part of these toxins forms a channel allowing for the translocation of the toxic N-terminal part, which is subsequently cleaved off at the cytoplasmic face of an intracellular compartment, most likely endosomes.
Aurora B expression correlates with aggressive behaviour in glioblastoma multiforme (2007)

Chromosomal abnormalities and genomic instability are common features of, and possible driving forces in, tumorigenesis. Recently, several mitotic proteins that are critical to proper chromosome segregation have been identified. Members of the Aurora kinase family have been identified as having important roles in mitosis; overexpression induces multicellularity and fosters polyploidy. As aneuploidy is a common feature of malignant gliomas, particularly glioblastomas (GBMs), we examined 25 prospectively collected GBMs to assess the role that overexpression of one member of this family, Aurora B, might have in the clinical behaviour of GBMs. Aurora B expression levels were markedly correlated with a shortened survival. Aurora B expression was not directly related to age, tumour proliferation status or to several common molecular changes found in GBMs. These results suggest that Aurora B may be a prognostic feature of impaired survival and a novel therapeutic target in some patients.
The comparative in-vitro activity of eight newer quinolones and nalidixic acid (1986)

The in-vitro antibacterial activity of nalidixic acid and the 4-quinolones, ciprofloxacin, norfloxacin, enoxacin, ofloxacin, pefloxacin, A-56619, A-56620 and CI-934 was assessed by determination of MICs. The 4-quinolones were all highly active against most isolates of Enterobacteriaceae, including nalidixic acid-resistant strains. Ciprofloxacin (MICs 0.002-2 mg/l) was the most active and A-56619 (MICs 0.008-32 mg/l) was the least active. A-56619, A-56620, ofloxacin, ciprofloxacin and CI-934 were highly active against Acinetobacter strains, pefloxacin and enoxacin were slightly less active, and a few strains were resistant to norfloxacin. All the compounds, including nalidixic acid, were active against Aeromonas strains (MICs 0.001-0.12 mg/l). Ciprofloxacin (MICs 0.06-1 mg/l) was the most active compound against Pseudomonas aeruginosa; A-56619 and CI-934 (MICs 1-16 mg/l) were the least active against this species. All the compounds were highly active against Haemophilus influenzae, Branhamella catarrhalis and Neisseria gonorrhoeae but the activity of all the compounds was poor against most isolates of Gardnerella vaginalis. All the 4-quinolones were active against staphylococci and CI-934 (MICs 0.03-0.25 mg/l) was the most active. CI-934 (MICs 0.06-2 mg/l) was also the most active compound against all streptococci. Most streptococci were sensitive also to ciprofloxacin (MICs 0.25-4 mg/l) but there were many isolates resistant to the other 4-quinolones. Against the anaerobic bacteria CI-934 was again the most active compound, particularly against the Gram-positive anaerobic cocci. Pefloxacin, enoxacin and norfloxacin had poor activity against most anaerobes. Ofloxacin, ciprofloxacin, A-56619 and A-56620 had good to moderate activity against all species of anaerobes except the Bacteroides fragilis group, against which none of the compounds was very active.
Semiconductor Nanorod Liquid Crystals and Their Assembly on a Substrate (2003)

The macroscopic alignment and superlattice structures of CdSe nanorods in a nematic liquid‐crystalline (LC) phase are determined by the phases that form prior to complete solvent evaporation (e.g., vortex structures in linear arrays, see Figure). By controlling the phase of the LC solution and its orientation using pretreated surfaces or external fields, it may be possible to achieve fine control of order in deposited nanorod films.