gebhart/patrepeval huggingface dataset:
Abstract 75: Electronic Integrated Monitoring of Medical Emergency Team Calls to a Step Down Unit (2007)

Purpose: Early discharge from intensive care units (ICU) to lower acuity monitoring units (step-down units or SDU) is increasing to improve care throughput. However, the ability of minimally invasive monitoring via electronic integrated monitoring systems (IMS) in SDUs to identify cardiopulmonary deterioration in order to activate Medical Emergency Team (MET) response is unknown. We evaluated the ability of an IMS index value to detect clinically significant events which might trigger activation of the MET earlier than called. Methods: Patients were monitored on a 24 bed trauma SDU according to prevailing policy and bedside decision making. An IMS (BioSign TM ) was inserted into the monitoring system and IMS data were collected in a blinded fashion for 8 weeks from all patients. The IMS uses 4 parameters (heart rate [HR], respiratory rate [RR], blood pressure [BP], peripheral oxygen saturation [SpO 2 ]) to develop a single neural networked signal known as the BioSign Index (BSI). Data were analyzed for patient deterioration according to both BSI trigger value and local MET criteria (DeVita, Qual Safety Health Care 2004). Results: Data from 333 patient admissions were evaluated reflecting 18,692 hours of continuous monitoring. SpO 2 monitoring data were absent in 38.5% of monitored hours, despite being mandatory. Most patients were stable throughout their SDU stay. MET activation occurred on 10 occasions (4 respiratory cause [2 SpO 2 , 2 SpO 2 +RR], 2 cardiac (BP), 1 mixed respiratory + cardiac, and 3 other (1 chest pain, 2 acute mental status change). All 7 MET events of respiratory and/or cardiac cause were detected by BSI in advance (mean advanced detection time prior to MET activation was 6.33 hours). Cardiopulmonary deterioration was generally characterized by progressive increases in BSI over time, not step increases. Conclusion : In this population, cardiopulmonary deterioration requiring MET activation was uncommon but always preceded by IMS index elevations which would have alerted the nursing staff in advance. Continuous monitoring of physiological variables may be augmented by integrating information from multiple parameters to create a new variable. The new index parameter may detect patient deterioration earlier than single parameter monitoring.
Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes (1999)

We have described the SART-1 gene-encoding peptides recognized by HLA-A2601-restricted and tumor-specific cytotoxic T lymphocytes (CTLs). We now have investigated whether SART-1 encodes peptides capable of inducing the HLA-A24-restricted CTLs. Among the 18 different peptides with HLA-A24-binding motifs, the SART-1(690-698) peptide (EYRGFTQDF) was most strongly recognized by the HLA-A24-restricted and tumor-specific CTLs established from an esophageal cancer patient. After a third stimulation in vitro, this peptide induced HLA-A24-restricted CTLs recognizing the SART-1(259)+ tumor cells in PBMCs of all HLA-A24 homozygous and the majority of HLA-A24 heterozygous cancer patients and healthy donors tested. A similar activity, induction of CTLs from PBMCs, was observed in the Saccharomyces cerevisiae-derived nonapeptide (EYRGFTPMF) that shares 7 amino acids with the SART-1(690-698) peptide. The SART-1(690-698) peptide-induced CTL activity was significantly higher in PBMCs of HLA-A24 homozygotes than in HLA-A24 heterozygotes. The CTL precursor frequency in PBMCs after a third stimulation in vitro with the SART-1(690-698) peptide was high (>1/200) in both cancer patients and healthy donors. The SART-1(690-698) peptide could thus be useful for specific immunotherapy of HLA-A24+ cancer patients.
Knowledge discovery and memory space as asymmetric information-the architecture of the Internet media lab netzspannung.org (2003)

In this paper we describe the elements of netzspannung.org, an on-line media laboratory as a Competence Centre for digital art, culture and technology. To this end netzspannung.org is being developed as an open distributed toolbox for knowledge discovery and experimentation with networked media resources accompanied by community, information channels and media strategies connecting processes in virtual space with real places. The first online demonstrator of netzspannung.org explores the creation of an architecture for visualising and exploring the interrelations between media art, culture, science aid technology. URL http://netzspannung.org.
Deletions of the Terminal Sequences in the Genomes of the White Pock (u) and Host-restricted (p) Mutants of Rabbitpox Virus (1980)

The DNAs of wild-type rabbitpox virus (u+.p+), and selected u (white pock, u.p+) and p (white pock, PK-15 cell non-permissive, u.p) mutants were compared by restriction enzyme analysis. The cleavage fragments produced by digestion with HindIII or EcoRI endonucleases were separated by agarose gel electrophoresis. Deletions of approx. 10 x 10(6) were recognized at the right-hand terminus of each u mutant (three isolates), whereas deletions of 5 x 10(6) to 20 x 10(6) were detected at the left-hand terminus of each p mutant (nine isolates), Rapidly renaturing terminal restriction fragments, indicative of the covalent cross-links, were only found at the terminus unaffected by deleted sequences. Recombinant viruses that were wild-type in both genetic characters and in restriction pattern were recovered from mixed infections involving u and p mutants, but no genetic interaction was detected between crosses involving only u or p mutants. The p mutants were divided into two non-permissive classes dependent on their biochemical expression in PK-15 cells: the 'early' class which failed to replicate virus DNA and synthesized only the 'early' polypeptides and the 'late' class which appeared to be normal both in virus DNA and polypeptide synthesis. There was no correlation between the extent of the left-hand terminal deletion and the extent of permissiveness of the p mutants in PK-15 cells.
Lectins and protein traffic early in the secretory pathway (2002)

Lectins of the early secretory pathway are involved in selective transport of newly synthesized glycoproteins from the endoplasmic reticulum (ER) to the ER-Golgi intermediate compartment (ERGIC). The most prominent cycling lectin is the mannose-binding type I membrane protein ERGIC-53 (ERGIC protein of 53 kDa), a marker for the ERGIC, which functions as a cargo receptor to facilitate export of an increasing number of glycoproteins with different characteristics from the ER. Two ERGIC-53-related proteins, VIP36 (vesicular integral membrane protein 36) and a novel ERGIC-53-like protein, ERGL, are also found in the early secretory pathway. ERGL may act as a regulator of ERGIC-53. Studies of ERGIC-53 continue to provide new insights into the organization and dynamics of the early secretory pathway. Analysis of the cycling of ERGIC-53 uncovered a complex interplay of trafficking signals and revealed novel cytoplasmic ER-export motifs that interact with COP-II coat proteins. These motifs are common to type I and polytopic membrane proteins including presenilin 1 and presenilin 2. The results support the notion that protein export from the ER is selective.
Comparing features from ECG pattern and HRV analysis for emotion recognition system (2016)

We propose new features for emotion recognition from short ECG signals. The features represent the statistical distribution of dominant frequencies, calculated using spectrogram analysis of intrinsic mode function after applying the bivariate empirical mode decomposition to ECG. KNN was used to classify emotions in valence and arousal for a 3-class problem (low-medium-high). Using ECG from the Mahnob-HCI database, the average accuracies for valence and arousal were 55.8% and 59.7% respectively with 10-fold cross validation. The accuracies using features from standard Heart Rate Variability analysis were 42.6% and 47.7% for valence and arousal respectively for the 3-class problem. These features were also tested using subject-independent validation, achieving an accuracy of 59.2% for valence and 58.7% for arousal. The proposed features also showed better performance compared to features based on statistical distribution of instantaneous frequency, calculated using Hilbert transform of intrinsic mode function after applying standard empirical mode decomposition and bivariate empirical mode decomposition to ECG. We conclude that the proposed features offer a promising approach to emotion recognition based on short ECG signals. The proposed features could be potentially used also in applications in which it is important to detect quickly any changes in emotional state.
The role of complement in initial gingivitis (1976)

The effects of decomplementation by Cobra Venom Factor (CVF) on cell migration and vascular permeability in experimentally induced initial gingival inflammation in the dog have been determined. Gingivitis was provoked in four Beagle dogs with normal gingiva during an eight hour period by topical application of an extract of human plaque. In each dog, the applications were performed on one side of the jaws before, and on the contralateral side after decomplementation. Following six and a half hours of plaque extract application, colloidal carbon was injected intravenously in one of the hind legs. At the end of the application period biopsies were sampled, the tissue sections were embedded in Epon and semi thin sections (2–3 μm) were prepared and examined in the light microscope regarding (1) amount of carbon containing vascular units subjacent to the junctional epithelium (2) number of neutrophils and mononudear leukocytes within the junctional epithelium. The present experiment indicates that (1) plaque extract contains substances which, when applied onto the margin of normal gingiva, mediate attraction of leukocytes and alterations of the function of the dento‐gingival vasculature i.e. induce symptoms of initial gingivitis. (2) in the decotnplemented animals the plaque components displayed a) a reduced capacity to influence vascular function but b) an almost unaltered leukocyte attracting potential with regard to neutrophils.
Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells. (1983)

The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted into the transfer vector pSV2-neo and introduced into various plasmacytoma and hybridoma cell lines. The transfer of the mu and kappa genes resulted in the production of pentameric, hapten-specific, functional IgM.
Isolation, Characterization, cDNA Cloning, and Antimicrobial Properties of Two Distinct Subfamilies of α-Defensins from Rhesus Macaque Leukocytes (1999)

Experiments to isolate and characterize rhesus macaque myeloid alpha-defensins (RMADs) were conducted. Seven RMAD peptides were isolated and sequenced, and the cDNAs encoding six of these peptides and one other alpha-defensin from bone marrow were also characterized. Four of the RMADs were found to be highly similar to human neutrophil alpha-defensins HNP-1 to HNP-3, while the remaining four peptides were much more similar to human enteric alpha-defensin HD-5. Two alpha-defensin pairs differed only by the presence or absence of an additional arginine at the amino termini of their mature peptides, indicative of alternate posttranslational processing. The primary translation products of RMAD-1 to -8 are 94- and 96-amino-acid prepropeptides that are highly similar to those of human alpha-defensins. Immunolocalization experiments revealed a granular cytoplasmic pattern in the cytoplasms of neutrophils, indistinguishable from the pattern observed after immunostaining of human myeloid alpha-defensins in polymorphonuclear leukocytes. Each of the purified peptides was tested for its in vitro activities against Staphylococcus aureus 502a, Listeria monocytogenes EGD, Escherichia coli ML35, and Cryptococcus neoformans 271A. Several of the peptides were microbicidal for the gram-positive bacteria and C. neoformans at defensin concentrations in the range of 2 to 5 microM. All of the peptides were bacteriostatic against E. coli, but none were bactericidal for this organism. This study is the first to characterize the sequences and activities of alpha-defensins from nonhuman primates, data that should aid in delineating the role of these peptides in rhesus macaque host defense.
Deubiquitinase USP9x Confers Radioresistance through Stabilization of Mcl-1 (2012)

Myeloid cell leukemia sequence 1 (Mcl-1), an antiapoptotic member of the Bcl-2 family, is often overexpressed in tumor cells limiting the therapeutic success. Mcl-1 differs from other Bcl-2 members by its high turnover rate. Its expression level is tightly regulated by ubiquitylating and deubiquitylating enzymes. Interaction of Mcl-1 with certain Bcl-2 homology domain 3 (BH3)-only members of the Bcl-2 family can limit the access to Mcl-1 ubiquitin ligase E3 and stabilizes the antiapoptotic protein. In addition, the overexpression of the deubiquitinase ubiquitin-specific protease 9x (USP9x) can result in the accumulation of Mcl-1 by removing poly-ubiquitin chains from Mcl-1 preventing its proteasomal degradation. Analyzing radiation-induced apoptosis in Jurkat cells, we found that Mcl-1 was downregulated more efficiently in sensitive parental cells than in a resistant subclone. The decline of Mcl-1 correlated with cell death induction and clonogenic survival. Knockdown of BH3-only proteins Bim, Puma, and Noxa did not affect Mcl-1 level or radiation-induced apoptosis. However, ionizing radiation resulted in activation of USP9x and enhanced deubiquitination of Mcl-1 in the radioresistant cells preventing fast Mcl-1 degradation. USP9x knockdown enhanced radiation-induced decrease of Mcl-1 and sensitized the radioresistant cells to apoptosis induction, whereas USP9x knockdown alone did not change Mcl-1 level in unirradiated cells. Together, our results indicate that radiation-induced activation of USP9x inhibits Mcl-1 degradation and apoptosis resulting in increased radioresistance.
Template synthesis of macromolecules. Synthesis and chemistry of functionalized macroporous poly(divinylbenzene) (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTemplate synthesis of macromolecules. Synthesis and chemistry of functionalized macroporous poly(divinylbenzene)Kenneth J. Shea, Evan A. Thompson, Sue D. Pandey, and Philip S. BeauchampCite this: J. Am. Chem. Soc. 1980, 102, 9, 3149–3155Publication Date (Print):April 1, 1980Publication History Published online1 May 2002Published inissue 1 April 1980https://pubs.acs.org/doi/10.1021/ja00529a044https://doi.org/10.1021/ja00529a044research-articleACS PublicationsRequest reuse permissionsArticle Views438Altmetric-Citations79LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Comprehensive copy number profiles of breast cancer cell model genomes (2006)

The intrinsic genetic differences between these cell lines will influence their biologic and pharmacologic response as an experimental model. Knowledge of segmental changes in these genomes deduced from our study will facilitate the interpretation of biological data derived from such cells.
Swelling of Hydroxypropyl Methylcellulose Matrix Tablets. 2. Mechanistic Study of the Influence of Formulation Variables on Matrix Performance and Drug Release (1996)

We characterized the effect of hydroxypropyl methylcellulose (HPMC)/lactose ratio and HPMC viscosity grade (molecular weight) on solute release and swelling of matrix tablets. We used a semiquantitative optical imaging method to monitor the swelling of matrices with HPMC content from 20% to 80% (w/w) and four viscosity grades. Several aspects of the swelling process common to all formulations were revealed: (i) swelling is anisotropic with a preferential expansion in the axial direction, (ii) swelling is isotropic with respect to the gel layer thickness and composition in both axial and radial directions, (iii) the gel layer develops in three stages, and (iv) water penetration is Fickian in nature and essentially constant for all formulations. We monitored simultaneously drug, lactose, and HPMC release. Lactose and drug release rates were superimposed, indicating a similar diffusional release mechanism and no interaction with HPMC. The strong dependence of HPMC release on viscosity grade is explained on the basis of the concept of polymer disentanglement concentration. We analyzed drug release rates using a model for a reservoir-type release system that incorporates swelling kinetics. HPMC/lactose ratio modulates drug release rate by altering drug diffusivity, a function of gel composition. In contrast, HPMC viscosity grade impacts matrix dissolution and gel layer thickness development below a critical molecular weight. For slowly dissolving matrices containing high viscosity grade (> 4000 cps) HPMC, similar drug release rates are observed mainly due to the same drug diffusivity as a result of the identical gel composition and thickness. For fast dissolving matrices (< or = 100 cps) swelling inhomogeneity is proposed as being responsible for a higher apparent drug diffusivity and release rate.
Profile: Veebot [Resources_Start-ups] (2013)

You probably know the routine for drawing blood. A medical technician briefly wraps your arm in a tourniquet and looks your veins over, sometimes tapping gently with a gloved finger on your inner elbow. Then the med tech selects a target.
China-Reduced Tail Risk Provides Entrance Opportunity (2015)

Macro:China to pursue reforms China is likely to retain the focus on re-orienting its economy towards consumption,accepting a slower rate of growth.We expect growth to decelerate to around 7%,from an estimated 7.4%in 2014.Faster growth in the US and Europe should help sustain exports while policymakers maintain a tight leash
Clinical and radiographic delineation of Bent Bone Dysplasia‐FGFR2 type or Bent Bone Dysplasia with Distinctive Clavicles and Angel‐shaped Phalanges (2016)

Bent Bone Dysplasia-FGFR2 type is a relatively recently described bent bone phenotype with diagnostic clinical, radiographic, and molecular characteristics. Here we report on 11 individuals, including the original four patients plus seven new individuals with three longer-term survivors. The prenatal phenotype included stillbirth, bending of the femora, and a high incidence of polyhydramnios, prematurity, and perinatal death in three of 11 patients in the series. The survivors presented with characteristic radiographic findings that were observed among those with lethality, including bent bones, distinctive (moustache-shaped) small clavicles, angel-shaped metacarpals and phalanges, poor mineralization of the calvarium, and craniosynostosis. Craniofacial abnormalities, hirsutism, hepatic abnormalities, and genitourinary abnormalities were noted as well. Longer-term survivors all needed ventilator support. Heterozygosity for mutations in the gene that encodes Fibroblast Growth Factor Receptor 2 (FGFR2) was identified in the nine individuals with available DNA. Description of these patients expands the prenatal and postnatal findings of Bent Bone Dysplasia-FGFR2 type and adds to the phenotypic spectrum among all FGFR2 disorders. © 2016 Wiley Periodicals, Inc.
Advantage of Dimeric Peptide Antigens in Serodiagnosis of HIV‐1 Infection (1997)

The reactivity of antibodies with dimeric and monomeric peptide antigens was compared by ELISA. A panel of highly purified synthetic peptides of HIV-1 representing defined regions, 598-609 and 524-533 (fusion domain) of gp41 and 306-320 of gp120, were used as antigens in the ELISA. These peptides were selected and synthesized taking into account the level of sequence conservation of various strains and hydrophilicity. The analysis included sera from 52 HIV-1 infected individuals and 53 HIV-1 negative controls. Both peptides from gp41 were found to be particularly immunoreactive with sera from HIV-1 infected individuals. The frequency of reactivity to the selected peptide from gp120 (V3 loop) in infected individuals was 82%. An interesting observation was that the dimeric peptide antigens had a detection rate more than 4-fold higher than the monomeric antigens. We found that lower levels of antibodies could be detected with dimeric antigens. The peptides reacted with few sera other than HIV-1 positive sera. These results implicate the potential dimeric peptide antigens to be utilized in the serodiagnosis of HIV-1 infection.
Safety of anthrax vaccine: an expanded review and evaluation of adverse events reported to the Vaccine Adverse Event Reporting System (VAERS) (2004)

This review produced no evidence for an unusual rate of any SAE or OMIAE attributable to AVA. It supported an earlier impression that AVA may cause significant local inflammation and should be administered over the deltoid rather than the triceps to avoid direct or compression injury to the ulnar nerve. The subjects of VAERS reports tended to be older than all recipients of AVA. Females generally had and/or reported AEs more often than males, but transient articular reactions were surprisingly more common in males. Variations in the frequency or severity (as judged by hospitalization and/or loss of duty) of reported AEs did not suggest a significant problem with (1) a particular lot of AVA, (2) recurrent AEs after multiple doses or (3) vaccination of persons with a concomitant illness or those given other vaccines or medications.
PCR Detection of Porcine circovirus type 2 DNA in Whole Blood, Serum, Oropharyngeal Swab, Nasal Swab, and Feces from Experimentally Infected Pigs and Field Cases. (2003)

Porcine circovirus type 2 (PCV2) shedding patterns were investigated by polymerase chain reaction (PCR) for the detection of PCV2 DNA, and the diagnostic suitability of a sample for the PCR was examined by using different types of samples. In the experimental infection, sixteen pigs were inoculated intranasally with PCV2. The samples, including oropharyngeal and nasal swabs, feces, whole blood and serum became positive for PCV2 DNA by PCR immediately after the inoculation, and almost all samples remained positive during the observation period, post-inoculation-day 70. Field samples were collected from 313 pigs in five different age groups. The overall percentages of positive samples in the whole blood, nasal swabs, and feces detected by PCR were 30.4%, 19.2%, and 20.4%, respectively. The frequency of positive samples increased after the nursery stages and reached a peak in the 3 to 4-month-old pigs. These results indicate that PCV2 infection may occur after weaning, that PCV2 DNA may be present in whole blood for a long period after infection, and that whole blood and serum are the most suitable sample types for the PCR analysis of PCV2.