gebhart/patrepeval huggingface dataset:
Evidence that the penton base of adenovirus is involved in potentiation of toxicity of Pseudomonas exotoxin conjugated to epidermal growth factor. (1984)

When KB cells are incubated for 1 h with human adenovirus type 2 or type 5 (1 microgram/ml) and a conjugate of epidermal growth factor and Pseudomonas exotoxin (EGF-PE), protein synthesis is inhibited by 80 to 90%. Under these conditions, neither adenovirus nor EGF-PE alone has any effect on host protein synthesis. Thus, adenovirus enhances the toxicity of EGF-PE. A number of antibodies to intact virus and capsid components were tested for their ability to block the enhancing activity and virus uptake. At appropriate dilutions, antibodies prepared against intact virus and penton base blocked the enhancing activity without affecting virus uptake. Antibodies against hexon and fiber blocked virus uptake and enhancing activity in parallel. These studies suggest that the penton base is important in lysis of the vesicles which contain adenovirus and EGF-PE, and this base allows virus and toxin to enter the cytoplasm.
Leucocyte typing III : white cell differentiation antigens (1987)

C Milstein: Introductory remarks Frances Gotch: Workshop structure and protocols D J Spiegelhalter & W R Gilks: Statistical analysis Bernadette Kienzle, Shirley Ellis, Winston Verbi, Michael Crumpton, Andrew McMichael, & Robert Knowles: Biochemical analysis of the Workshop antibodies with unknown specificities Andrew J McMichael & Frances Gotch: T-cell antigens: new and previously defined clusters Lewis L Lanier, An My Le, Athena H Ding, & Elizabeth L Evans: Analysis of the Workshop T-cell monoclonal antibodies by 'indirect two-colour immunofluorescence' and multiparameter flow cytometry Soo Young Yang: Biochemical analysis of the Workshop antibodies of the T-cell antigen and activation antigen panels T-cell antigens - papers N R Ling, I C M MacLennan, & D Y Mason: B-cell antigens: new and previously defined clusters B-cell antigens - papers P C L Beverley: Activation antigens: new and previously defined clusters Activation antigens - papers Nancy Hogg & Michael A Horton: Myeloid antigens: new and previously defined clusters Myeloid antigens - papers Michael A Horton & Nancy Hogg: Platelet antigens: new and previously defined clusters Platelet antigens - papers Steve Cobbold, Geoff Hale, & Herman Waldmann: Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters Non-lineage, LFA-1 family, and leucocyte common antigens - papers Plenary papers Nomenclature.
Teratogenicity of a mutagenised Rift Valley fever virus (MVP 12) in sheep. (2002)

A 5-fluorouracil mutagenised Rift Valley fever virus strain, which was shown to be attenuated and immunogenic in cattle and sheep, was evaluated for its ability to cause teratogenic effects in pregnant sheep. A group of 50 sheep at various stages of pregnancy was inoculated with the virus and the pregnancies followed to term. There were two abortions and 14% of the lambs produced by vaccinated ewes showed teratogenic effects, the most prevalent being spinal hypoplasia, hydranencephaly, brachygnathia inferior and arthrygryposis. The foetal malformations of the central nervous and musculo-skeletal systems were mostly consistent with those observed in sheep vaccinated with the attenuated Smithburn RVF strain. The teratogenic effects of MVP12 were not seen in previous experiments by other authors as immunisation of sheep took place in the second to third trimester of pregnancy, when the foetal brain tissue has completed most of its cell division.
Electrolyte Systems for High Withstand Voltage and Durability II. Alkylated Cyclic Carbonates for Electric Double-Layer Capacitors (2011)

This study describes new electrolyte systems that utilize alkylated cyclic carbonates, with a primary focus on getting a higher withstand voltage for electric double-layer capacitors (EDLCs). We attempted to increase the oxidative durability of carbonate solvents by protecting the 4th and/or 5th positions of the five-membered carbonate ring; protection was achieved by substituting those positions with small alkyl group(s). We investigates six different types of cyclic carbonates, viz., ethylene carbonate (EC), propylene carbonate (PC), butylene carbonate (BC), 2,3-butylene carbonate (2,3BC), isobutylene carbonate (iBC), and pentylene carbonate (PlC) have been investigated with regard to the electrochemical stability, as well as the melting point, boiling point, dielectric constant, viscosity, and the solubility of electrolyte salts. As a result, 2,3BC remained as the best potential candidate for an alternative solvent for EDLCs. 2,3BC has a high boiling point (243°C) that is comparable to PC (242°C) and dissolved spirobipyrrolidinium tetrafluoroborate (SBP-BF4). A SBP-BF4/2,3BC system showed a stabilized capacitance within wider voltage windows (ΔV = 3.5 V) that far exceeded that of conventional PC based systems (ΔV = 2.7 V). This high withstand voltage is caused mainly by the outstanding oxidative durability of 2,3BC.
Characterization of two monoclonal antibodies reactive with the external domain of the platelet-derived growth factor receptor. (1988)

Two monoclonal antibodies against the receptor for platelet-derived growth factor (PDGF) were obtained by immunizing mice with pure PDGF receptor preparations derived from porcine uterus. The antibodies, denoted PDGFR-B1 and PDGFR-B2, both bound to the external domain of the receptor, as demonstrated by indirect immunofluorescence and binding of 125I-labeled antibodies to intact human fibroblasts. Both antibodies precipitated pure 175-kDa 32P-labeled autophosphorylated porcine PDGF receptor as well as a Mr 175,000 glycoprotein from metabolically labeled cells. The monoclonal antibodies did not inhibit binding of 125I-PDGF to human fibroblasts and did not stimulate these cells to undergo mitosis. Both antibodies induced clustering and down-regulation of their antigen. However, this resulted in only a partial loss of cell surface binding sites for PDGF itself, consistent with the conclusion that the monoclonals recognized only one of two or several receptors for PDGF. Clustering and down-regulation were not seen when the cells were incubated with monovalent Fab' fragments of the PDGFR-B2 antibody. The antibodies also stimulated autophosphorylation of pure PDGF receptor, and PDGFR-B2 was shown to stimulate phosphorylation of phosphofructokinase, an exogenous substrate for the PDGF receptor kinase. High concentrations of PDGFR-B2 antibody, or Fab' fragments thereof, failed to enhance the PDGF receptor kinase activity, compatible with the possibility that dimerization was of importance in the antibody-stimulated kinase activity of purified PDGF receptors.
A Facile Preparation of N-(Isopropoxyalkyl) Amides by Generation and Trapping of N-Acyliminium Ions from Ionization-Rearrangement Reactions of N-Triflyloxy Amides (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Facile Preparation of N-(Isopropoxyalkyl) Amides by Generation and Trapping of N-Acyliminium Ions from Ionization-Rearrangement Reactions of N-Triflyloxy AmidesRobert V. Hoffman and Naresh K. NayyarCite this: J. Org. Chem. 1994, 59, 13, 3530–3539Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://pubs.acs.org/doi/10.1021/jo00092a007https://doi.org/10.1021/jo00092a007research-articleACS PublicationsRequest reuse permissionsArticle Views856Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Enhancement of hot-electron generation rate in Schottky source metal–oxide–semiconductor field-effect transistors (2000)

Source-side hot-electron generation is experimentally demonstrated in Schottky source metal–oxide–semiconductor field-effect transistors (MOSFETs). An asymmetric n-type MOSFET having a CoSi2 layer in place of one of the n+ source/drain regions has been fabricated and intensively investigated. When the CoSi2 layer is used as the source, large gate current and negative-differential conductance (NDC) are simultaneously observed, whereas, when the n+ region is used as the source, both gate current and NDC are not observed. By comparing the device characteristics before and after the NDC observation, it is concluded that the gate current is due to hot electrons generated at the Schottky source side and the NDC is caused by trapped electrons in the oxide. These source-side hot electrons will open up the way to the realization of deca-nanoscaled high-speed devices.
2-hydroxyestrone: the 'good' estrogen. (1996)

The issue of the role of 2-hydroxyestrone (2-OHE1) in breast cancer has been the subject of considerable controversy as to whether it is carcinogenic or anticarcinogenic. The expanding data base outlined below is most consistent with the conclusion that 2-OHE1 is anticarcinogenic. In every experimental model in which 2-hydroxylation was increased, protection against tumors was achieved. Correspondingly, when 2-hydroxylation was decreased, an increase in cancer risk was observed. Even more dramatically, in the case of laryngeal papillomas induction of 2-hydroxylation with indole-3-carbinol (I3C) has resulted in inhibition of tumor growth during the time that the patients continue to take 13C or vegetables rich in this compound.
Reconfigurable Low Area Complexity Filter Bank Architecture Based on Frequency Response Masking for Nonuniform Channelization in Software Radio Receivers (2011)

The most computationally demanding block in the digital front-end of a software defined radio (SDR) receiver is the channelizer which operates at the highest sampling rate. Channelizers are employed in the SDR receivers for extracting individual channels (frequency bands) from the digitized wideband input signal. Reconfigurability and low complexity are the two key requirements in an SDR channelizer. A new reconfigurable filter bank (FB) architecture based on frequency response masking (FRM) for SDR channelizers is proposed. The proposed FB offers reconfigurability at the architectural level and at the channel filter level and is capable of extracting channels of nonuniform bandwidths corresponding to multiple wireless communication standards from the digitized wideband input signal. Design examples show that the proposed FB offers multiplier complexity reduction of 84% over the conventional per-channel (PC) approach, which is best suitable for the extraction of channels of nonuniform bandwidth. The proposed FB has been synthesized on 0.18 μm complementary metal oxide semiconductor (CMOS) technology and compared with the PC approach. Synthesis results show that the proposed FB offers area reduction of 85%, power reduction of 48.5%, and improvement in speed of 56.7% over the PC approach. The proposed FB has been implemented on Xilinx Virtex 2v3000ff1152-4 FPGA and tested using real-time inputs from a vector signal generator.
Certolizumab Pegol for the Treatment of Crohn's Disease (2007)

In patients with moderate-to-severe Crohn's disease, induction and maintenance therapy with certolizumab pegol was associated with a modest improvement in response rates, as compared with placebo, but with no significant improvement in remission rates. (ClinicalTrials.gov number, NCT00152490 [ClinicalTrials.gov].).
The lysyl oxidase inhibitor β-aminopropionitrile reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats (2015)

Extracellular matrix (ECM) remodelling of the adipose tissue plays a pivotal role in the pathophysiology of obesity. The lysyl oxidase (LOX) family of amine oxidases, including LOX and LOX-like (LOXL) isoenzymes, controls ECM maturation, and upregulation of LOX activity is essential in fibrosis; however, its involvement in adipose tissue dysfunction in obesity is unclear. In this study, we observed that LOX is the main isoenzyme expressed in human adipose tissue and that its expression is strongly upregulated in samples from obese individuals that had been referred to bariatric surgery. LOX expression was also induced in the adipose tissue from male Wistar rats fed a high-fat diet (HFD). Interestingly, treatment with β-aminopropionitrile (BAPN), a specific and irreversible inhibitor of LOX activity, attenuated the increase in body weight and fat mass that was observed in obese animals and shifted adipocyte size toward smaller adipocytes. BAPN also ameliorated the increase in collagen content that was observed in adipose tissue from obese animals and improved several metabolic parameters - it ameliorated glucose and insulin levels, decreased homeostasis model assessment (HOMA) index and reduced plasma triglyceride levels. Furthermore, in white adipose tissue from obese animals, BAPN prevented the downregulation of adiponectin and glucose transporter 4 (GLUT4), as well as the increase in suppressor of cytokine signaling 3 (SOCS3) and dipeptidyl peptidase 4 (DPP4) levels, triggered by the HFD. Likewise, in the TNFα-induced insulin-resistant 3T3-L1 adipocyte model, BAPN prevented the downregulation of adiponectin and GLUT4 and the increase in SOCS3 levels, and consequently normalised insulin-stimulated glucose uptake. Therefore, our data provide evidence that LOX plays a pathologically relevant role in the metabolic dysfunction induced by obesity and emphasise the interest of novel pharmacological interventions that target adipose tissue fibrosis and LOX activity for the clinical management of this disease.
Protein truncation test (PTT) for rapid detection of translation-terminating mutations (1993)

Currently available techniques used to recognize point mutations in genetic disease are time consuming and are capable of screening only small pieces of DNA. Moreover, they detect all sequence differences including phenotypically silent changes. Consequently, they are not convenient to analyse mutations in large, multi-exonic genes, where a large fraction of pathological point mutations arises from early termination, as is the case for the one third non-deletion/duplication cases of Duchenne Muscular Dystrophy. We have developed a rapid and sensitive method, the Protein Truncation Test (PTT). PTT is based on a combination of RT-PCR, transcription and translation and selectively detects translation-terminating mutations. We demonstrate its effectiveness to detect point mutations in DMD-patients and carrier females. PTT should be widely applicable diagnostically in any disease where early terminations contribute substantially to the disease cause.
Myocyte enhancer factor 2 (MEF2) binding site is essential for C2C12 myotube-specific expression of the rat GLUT4/muscle-adipose facilitative glucose transporter gene. (1994)

We have cloned and characterized the rat GLUT4 gene in order to identify the cis-DNA elements responsible for tissue-specific GLUT4 expression. In this study, a variety of luciferase reporter gene constructs were transiently transfected into C2C12 myoblasts and myotubes as a model for skeletal muscle differentiation. These data identified a 103-base pair fragment, located from -522 to -420 relative to the transcription initiation site, that was sufficient to account for GLUT4 C2C12 myotube-specific expression. This fragment was operationally defined as an enhancer since it conferred myotube-specific expression in the context of both the minimal native GLUT4 or the heterologous thymidine kinase promoters in an orientation-independent manner. Further, mutagenesis of this fragment demonstrated that a sequence analogous to the muscle creatine kinase myocyte enhancer factor 2 (MEF2) binding site (-466 and -457) was required for transcriptional activation. Electrophoretic mobility gel shift assays demonstrated specific binding activity to the GLUT4 MEF2 sequences which directly correlated with functional expression. Although this element was capable of directing myotube-specific expression when cloned as multiple copies into luciferase reporter gene constructs, the MEF2 sequence alone was insufficient to enhance GLUT4 expression. These data demonstrated that GLUT4 muscle-specific expression is conferred by a 103-base pair DNA sequence located between -522 and -420 of rat GLUT4 gene. This region encompasses a MEF2 binding site which was necessary, but not sufficient, for transcriptional activation.
Adenoviral-mediated gene transfer induces sustained pericardial VEGF expression in dogs: effect on myocardial angiogenesis (1999)

Adenoviral-mediated gene transfer is capable of inducing sustained VEGF165 expression in the pericardium; however, locally targeted pericardial VEGF delivery failed to improve myocardial collateral perfusion in this model.
MALE INFERTILITY (2000)

No AccessJournal of UrologyUROLOGICAL SURVEY: ABSTRACTS1 Feb 2000MALE INFERTILITY Jonathan P. JarowM.D. Jonathan P. JarowJonathan P. Jarow More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)67954-2AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail References 1 : Background of the recognition of Klinefelter’s syndrome as a distinct pathologic entity. Am J Obst Gynecol1973; 116: 436. Google Scholar 2 : Preimplantation diagnosis for X and Y normality in embryos from three Klinefelter patients. Hum Reprod1996; 11: 1650. Google Scholar 3 : Testicular sperm recovery in nine 47, XXY Klinefelter patients. Hum Reprod1996; 11: 1644. Google Scholar 1 : The prevalence of seminal vesicle cysts in autosomal dominant polycystic kidney disease. Nephrol Dial Transpl1998; 13: 2825. Google Scholar 1 : Sperm survival and recovery: two tests that predict in vitro fertilization outcome. Fertil Steril1994; 62: 1244. Google Scholar 1 : Is intracytoplasmic sperm injection (ICSI) a safe procedure? What do we learn from early pregnancy data about ICSI?. Hum Reprod1996; 11: 440. Google Scholar 2 : Prospective follow-up study of 423 children born after intracytoplasmic sperm injection. Hum Reprod1996; 11: 1558. Google Scholar © 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 163Issue 2February 2000Page: 667-671 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.MetricsAuthor Information Jonathan P. Jarow More articles by this author Expand All Advertisement Loading ...
A New Method for Measuring Dielectric Constant Using the Resonant Frequency of a Patch Antenna (1986)

An analytical expression is given for the resonant frequency of a rectangular patch antenna. It shows explicitly the dependence of the resonant frequency on the characteristic parameters of a patch antenna. Based on this result, a new method is developed for the measurement of the dielectric constant of a thin slab substrate. Basically, the test equipment consists of a rectangular microstrip antenna the patch of which is fed either by a microstrip line or coaxial line. From the measured resonance parameters of the rectangular patch antenna, the dielectric constant can be easily obtained. The measured values of the present method are in agreement with the precision standard cavity resonator method. Accuracy of the dielectric constant so obtained is satisfactory.
Synthesis and characterization of tungsten oxo neopentylidene complexes (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and characterization of tungsten oxo neopentylidene complexesJeffrey H. Wengrovius and Richard R. SchrockCite this: Organometallics 1982, 1, 1, 148–155Publication Date (Print):January 1, 1982Publication History Published online1 May 2002Published inissue 1 January 1982https://pubs.acs.org/doi/10.1021/om00061a026https://doi.org/10.1021/om00061a026research-articleACS PublicationsRequest reuse permissionsArticle Views842Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Shigella flexneri 2a Strain CVD 1207, with Specific Deletions in virG , sen , set , and guaBA , Is Highly Attenuated in Humans (2000)

A phase 1 clinical trial was conducted among 35 healthy adult volunteers to evaluate the safety, immunogenicity, and shedding of different doses of CVD 1207, a live attenuated Shigella flexneri 2a vaccine candidate with specific deletion mutations in virG, sen, set, and guaBA. CVD 1207 retains the ability to invade epithelial cells but cannot effectively spread intercellularly after invasion (DeltavirG), does not produce enterotoxin (Deltasen and Deltaset), and has limited proliferation in vivo (DeltaguaBA). In a consecutive fashion, groups of three to seven subjects ingested a single oral dose of CVD 1207 at an inoculum of either 10(6), 10(7), 10(8), 10(9), or 10(10) CFU. CVD 1207 was remarkably well-tolerated at inocula as high as 10(8) CFU. In comparison, one of 12 subjects who received 10(9) CFU experienced mild diarrhea and another experienced a single episode of emesis. One of five subjects who received 10(10) CFU experienced watery diarrhea and emesis. All subjects who ingested doses of 10(8) to 10(10) CFU excreted the vaccine; in 23 of 25, the duration of excretion was </=3 days. A dose-related, immunoglobulin A antibody-secreting cell (ASC) response to S. flexneri 2a O-specific lipopolysaccharide was seen, with geometric mean peak values of 6.1 to 35.2 ASCs/10(6) peripheral blood mononuclear cells (PBMC) among recipients of 10(7) to 10(10) CFU. The cytokine response to Shigella-specific antigens observed in volunteers' PBMC following vaccination suggested a Th1 pattern with stimulation of gamma interferon and absence of interleukin 4 (IL-4) or IL-5. CVD 1207 represents a Shigella live oral vaccine strain prepared from wild-type S. flexneri 2a by rational use of recombinant DNA technology that achieves a remarkable degree of attenuation compared with earlier recombinant strains, even when administered at high dosage.