gebhart/patrepeval huggingface dataset:
Noncontact Method for Sleep Stage Estimation (2004)

This paper describes a novel method to estimate sleep stage through noninvasive and unrestrained means. The Rechtschaffen and Kales (R-K) method is a standard to estimate sleep stage. However, it involves restraining the examinee and, thus, induces psychological stress. Furthermore, it requires specialists with a high degree of technical expertise and the use of an expensive polygraph. The sleep estimation method presented here is based on the noninvasive and unrestrained pneumatic biomeasurement method presented by the authors. Sleep stage transition in overnight sleep and the relationship between sleep stage and biosignals measured using the pneumatic method was analyzed and from the results, a mathematical model of sleep was created. Based on this model, a sleep stage estimator, including a sleep stage classifier and observer, was designed. The sleep state transition equation was the basis for the design of this observer, while the observed relationships were the basis for designing a classifier. Agreement of the estimated sleep stages with those obtained using the R-K method for the non-REM stage was 82.6%, for the REM stage was 38.3% and for Wake was 70.5%, including disagreement. However, the new method might ultimately result in better estimation of sleep stage due to the fact that it does not physically restrain the patient and does not induce psychological stress.
Gate Control of Spin-Orbit Interaction in an Inverted In0.53Ga0.47As/In0.52Al0.48As Heterostructure (1997)

We have confirmed that a spin-orbit interaction in an inverted I${\mathrm{n}}_{0.53}$G${\mathrm{a}}_{0.47}$As/I${\mathrm{n}}_{0.52}$A${\mathrm{l}}_{0.48}$As quantum well can be controlled by applying a gate voltage. This result shows that the spin-orbit interaction of a two-dimensional electron gas depends on the surface electric field. The dominant mechanism for the change in the spin-orbit interaction parameter can be attributed to the Rashba term. This inverted I${\mathrm{n}}_{0.53}$G${\mathrm{a}}_{0.47}$As/I${\mathrm{n}}_{0.52}$A${\mathrm{l}}_{0.48}$As heterostructure is one of the promising materials for the spin-polarized field effect transistor which is proposed by Datta and Das [Appl. Phys. Lett. 56, 665 (1990)].
Macrophage Inflammatory Protein–1α (Not T Helper Type 2 Cytokines) Is Associated with Severe Forms of Respiratory Syncytial Virus Bronchiolitis (2001)

It has been suggested that the pathogenesis of respiratory syncytial virus (RSV) infection is related to the development of T helper (Th) type 2 cytokine responses. The presence of Th1 and Th2 cytokines and the chemokines macrophage inflammatory protein (MIP)-1alpha and monocyte chemotactic protein (MCP)-1 were assessed by ELISA in nasopharyngeal secretions of infants with RSV infection. Infants with mild bronchiolitis had increased Th1 cytokines and reduced Th2 cytokines, compared with infants with upper respiratory tract illness alone. Severe bronchiolitis was characterized by a more balanced Th1-Th2 response that did not differ from that of infants with upper respiratory tract illness alone. In contrast, MIP-1alpha was markedly increased in infants with severe bronchiolitis. MIP-1alpha and MCP-1 levels also were inversely related to oxygen saturation (P<.005). Thus, the severity of RSV bronchiolitis appears to be related more to chemokine release than to Th2 cytokine production.
A Role for the WWOX Gene in Prostate Cancer (2006)

Abstract Expression of the WWOX gene, encompassing the common chromosome fragile site FRA16D, is altered in a large fraction of cancers of various types, including prostate cancer. We have examined expression and biological functions of WWOX in prostate cancer. WWOX mRNA and protein expression were significantly reduced in prostate cancer-derived cells (LNCaP, DU145, and PC-3) compared with noncancer prostate cells (PWR-1E), and WWOX expression was reduced in 84% of prostate cancers, as assessed by immunohistochemical staining. Down-modulation of WWOX expression in the prostate cancer-derived cells is due to DNA hypermethylation in the WWOX regulatory region. Treatment with 5-aza-2′-deoxycytidine (AZA), a DNA methyltransferase inhibitor, and trichostatin A, a histone deacetylase inhibitor, led to increased WWOX mRNA and protein expression in prostate cancer-derived cells, most strikingly in DU145 cells. Transfection-mediated WWOX overexpression in DU145 cells suppressed colony growth (P = 0.0012), and WWOX overexpression by infection with Ad-WWOX virus induced apoptosis through a caspase-dependent mechanism and suppressed cell growth. Lastly, ectopic expression of WWOX by Ad-WWOX infection suppressed tumorigenicity of xenografts in nude mice, and intratumoral AZA treatment halted tumor growth. The data are consistent with a role for WWOX as a prostate cancer tumor suppressor and suggest that WWOX signal pathways should be further investigated in normal and cancerous prostate cells and tissues. (Cancer Res 2006; 66(13): 6477-81)
Tattoo-based potentiometric ion-selective sensors for epidermal pH monitoring (2012)

This article presents the fabrication and characterization of novel tattoo-based solid-contact ion-selective electrodes (ISEs) for non-invasive potentiometric monitoring of epidermal pH levels. The new fabrication approach combines commercially available temporary transfer tattoo paper with conventional screen printing and solid-contact polymer ISE methodologies. The resulting tattoo-based potentiometric sensors exhibit rapid and sensitive response to a wide range of pH changes with no carry-over effects. Furthermore, the tattoo ISE sensors endure repetitive mechanical deformation, which is a key requirement of wearable and epidermal sensors. The flexible and conformal nature of the tattoo sensors enable them to be mounted on nearly any exposed skin surface for real-time pH monitoring of the human perspiration, as illustrated from the response during a strenuous physical activity. The resulting tattoo-based ISE sensors offer considerable promise as wearable potentiometric sensors suitable for diverse applications.
Induction of human interferon gene expression is associated with a nuclear factor that interacts with the NF-kappa B site of the human immunodeficiency virus enhancer (1989)

The relationship between transcription of alpha and beta interferon (IFN-alpha and IFN-beta) genes and the interaction of IFN promoter-binding transcription factors has been examined in monoblastoid U937 cells following priming with recombinant IFN-alpha 2 (rIFN-alpha 2) and Sendai virus induction. Pretreatment of U937 cells with rIFN-alpha 2 prior to Sendai virus infection increased the mRNA levels of IFN-alpha 1, IFN-alpha 2, and IFN-beta as well as the final yield of biologically active IFN. Analysis of nuclear protein-IFN promoter DNA interactions by electrophoretic mobility-shift assays demonstrated increased factor binding to IFN-alpha 1 and IFN-beta regulatory domains, although no new induction-specific complexes were identified. On the basis of competition electrophoretic mobility-shift assay results, factors interacting with the IFN-alpha 1 and IFN-beta promoters appear to be distinct DNA-binding proteins. U937 factor binding was localized to the P2 domain (-64 to -55) of the IFN-beta regulatory element, a sequence motif with 80% homology to the recognition site of transcription factor NF-kappa B. Protein-DNA interactions within the IFN-beta P2 domain were, in fact, specifically competed by either excess homologous P2 fragment or the human immunodeficiency virus enhancer element which contains two duplicated NF-kappa B recognition sites. Hybrid promoter-chloramphenicol acetyltransferase fusion plasmids, containing either the IFN-beta regulatory element or the human immunodeficiency virus enhancer element linked to the simian virus 40 promoter, were analyzed for virus and phorbol ester inducibility in epithelial and lymphoid cells, respectively. In the 293 cell line, both plasmids were constitutively expressed but not virus inducible, while in Jurkat cells, chloramphenicol acetyltransferase activity from these plasmids was induced by tumor-promoting agent treatment. These experiments suggest that induction of IFN gene expression may be controlled in part by transcription regulatory proteins binding to an NF-kappa B-like site within the IFN-beta promoter.
Optical imaging techniques for point-of-care diagnostics (2012)

Improving access to effective and affordable healthcare has long been a global endeavor. In this quest, the development of cost-effective and easy-to-use medical testing equipment that enables rapid and accurate diagnosis is essential to reduce the time and costs associated with healthcare services. To this end, point-of-care (POC) diagnostics plays a crucial role in healthcare delivery in both developed and developing countries by bringing medical testing to patients, or to sites near patients. As the diagnosis of a wide range of diseases, including various types of cancers and many endemics, relies on optical techniques, numerous compact and cost-effective optical imaging platforms have been developed in recent years for use at the POC. Here, we review the state-of-the-art optical imaging techniques that can have a significant impact on global health by facilitating effective and affordable POC diagnostics.
Development of several organs that require inductive epithelial-mesenchymal interactions is impaired in LEF-1-deficient mice. (1994)

Lymphoid enhancer factor 1 (LEF-1) is a sequence-specific DNA-binding protein that is expressed in pre-B and T lymphocytes of adult mice, and in the neural crest, mesencephalon, tooth germs, whisker follicles, and other sites during embryogenesis. We have generated mice carrying a homozygous germ-line mutation in the LEF-1 gene that eliminates its protein expression and causes postnatal lethality. The mutant mice lack teeth, mammary glands, whiskers, and hair but show no obvious defects in lymphoid cell populations at birth. The LEF-1-deficient mice also lack the mesencephalic nucleus of the trigeminal nerve, the only neural crest-derived neuronal populations. Together, the pattern of these defects suggest an essential role for LEF-1 in the formation of several organs and structures that require inductive tissue interactions.
Purification and chemical characterization of the receptor for interleukin 2 from activated human T lymphocytes and from a human T-cell lymphoma cell line. (1984)

The cell surface receptor for interleukin 2 plays a central role in the biology of this T-cell growth factor. A combination of affinity chromatography, high-performance liquid chromatography, and NH2-terminal protein sequencing was used to purify and chemically characterize the interleukin 2 receptor both from phytohemagglutinin-activated T cells and from the human T-cell lymphoma cell line HuT-102. The receptor isolated from HuT-102 cells was purified 16,000-fold to homogeneity as evidenced by (i) a final specific activity close to the theoretical specific activity of 18,182 fmol of receptor per microgram of protein, (ii) a single band on polyacrylamide gel electrophoresis with an Mr of 55,000, and (iii) a unique, unambiguous NH2-terminal protein sequence. The receptor purified from phytohemagglutinin-activated T lymphocytes had an Mr of 60,000 but it had the same NH2-terminal protein sequence.
Electrocatalytic reduction of carbon dioxide mediated by Re(bipy)(CO)3Cl (bipy = 2,2′-bipyridine) (1984)

J. Hawecker, J. Lehn and R. Ziessel, J. Chem. Soc., Chem. Commun., 1984, 328 DOI: 10.1039/C39840000328
Hydrazinolysis of heparin and other glycosaminoglycans (1984)

Heparin, carboxy-group-reduced heparin, several sulphated monosaccharides and disaccharides formed from heparin, and a tetrasaccharide prepared from chondroitin sulphate were treated at 100 degrees C with hydrazine containing 1% hydrazine sulphate for periods sufficient to cause complete N-deacetylation of the N-acetylhexosamine residues. Under these hydrazinolysis conditions both the N-sulphate and the O-sulphate substituents on these compounds were completely stable. However, the uronic acid residues were converted into their hydrazide derivatives at rates that depended on the uronic acid structures. Unsubstituted L-iduronic acid residues reacted much more slowly than did unsubstituted D-glucuronic acid or 2-O-sulphated L-iduronic acid residues. The chemical modification of the carboxy groups resulted in a low rate of C-5 epimerization of the uronic acid residues. The hydrazinolysis reaction also caused a partial depolymerization of heparin but not of carboxy-group-reduced heparin. Treatment of the hydrazinolysis products with HNO2 at either pH 4 or pH 1.5 or with HIO3 converted the uronic acid hydrazides back into uronic acid residues. The use of the hydrazinolysis reaction in studies of the structures of uronic acid-containing polymers and the implications of the uronic acid hydrazide formation are discussed.
Human Autophagins, a Family of Cysteine Proteinases Potentially Implicated in Cell Degradation by Autophagy (2003)

We have cloned four human cDNAs encoding putative cysteine proteinases that have been tentatively called autophagins. These proteins are similar to Apg4/Aut2, a yeast enzyme involved in the activation of Apg8/Aut7 during the process of autophagy. The identified proteins ranging in length from 393 to 474 amino acids also contain several structural features characteristic of cysteine proteinases including a conserved cysteine residue that is essential for the catalytic properties of these enzymes. Northern blot analysis demonstrated that autophagins are broadly distributed in human tissues, being especially abundant in skeletal muscle. Functional and morphological analysis in autophagy-defective yeast strains lacking Apg4/Aut2 revealed that human autophagins-1 and -3 were able to complement the deficiency in the yeast protease, restoring the phenotypic and biochemical characteristics of autophagic cells. Enzymatic studies performed with autophagin-3, the most widely expressed human autophagin, revealed that the recombinant protein hydrolyzed the synthetic substrate Mca-Thr-Phe-Gly-Met-Dpa-NH(2) whose sequence derives from that present around the Apg4 cleavage site in yeast Apg8/Aut7. This proteolytic activity was diminished by N-ethylmaleimide, an inhibitor of cysteine proteases including yeast Apg4/Aut2. These results provide additional evidence that the autophagic process widely studied in yeast can also be fully reconstituted in human tissues and open the possibility to explore the relevance of the autophagin-based proteolytic system in the induction, regulation, and execution of autophagy.
Microalgae for “Healthy” Foods—Possibilities and Challenges (2010)

Microalgae have the potential to become a novel source of bioactive molecules, especially for those who might wish to enhance the nutritional and functional quality of foods. Spirulina, one of the most popular microalgae, has been described by the World Health Organization as one of the greatest superfoods on earth serving as an example of the potential of microalgae. This review provides background on current and future uses of microalgae in the human diet, lists the most common species of microalgae used to this end, and describes some production methods used in research and industrial production and recovery. The review also discusses some of the difficulties so far encountered such as low productivities and recovery rates, as well as challenges in the production of compounds of interest. Many scientists and engineers in research centers around the globe are currently dedicated to solve these problems as the various capabilities of microalgae have caught the attention of the energy, environmental, and agricultural industries, we propose that the food industry should as well evaluate the potential of microalgae as a novel source of "health promoting" compounds.
Single Crystalline Nanowires of Lead Can Be Synthesized through Thermal Decomposition of Lead Acetate in Ethylene Glycol (2003)

This paper describes a solution-phase route to the facile synthesis of lead nanowires with lateral dimensions as thin as 35 nm and lengths up to 250 μm. These lead nanowires were single crystalline in structure, and electron transport measurements on individual nanowires (using the four-probe method) indicate a phase transition to the superconducting state around 7.13 K, a temperature similar to the value (7.20 K) reported for bulk lead. Different from polycrystalline samples, no significant variation in the transition temperature was observed for nanowires with diameters down to 50 nm.
Micromachined modulator arrays for use in free-space optical communication systems (2004)

A summary is presented of some of the design criteria relevant to the realisation of silicon micromachined modulator arrays for use in free-space optical communication systems. Theoretical performance levels achievable are compared with values measured on experimental devices produced using a modified Multi-User MEMS Process (MUMPS). Devices capable of realising modulation rates in excess of 300 kHz are described and their optical characteristics compared with published data on devices based on multiple quantum well technology.
Genetic Regulation of Amifampridine Phosphate (Firdapse(R)) Metabolism Produces Significant Differences in Pharmacokinetics and Side Effects (P04.095) (2012)

Objective: To examine the pharmacogenomics of amifampridine phosphate in healthy volunteers. Background Amifampridine phosphate, a potassium channel blocking agent, is used in the treatment of Lambert Eaton myasthenic syndrome. We recently identified 3 N-acetyl amifampridine as its major metabolite. Genetic polymorphisms in N-acetyl-transferase 2 (NAT 2) genes determine acetylator status (“fast” or “slow”) and influence both toxicity and efficacy profiles of acetylated drugs. Design/Methods: An open label single center study enrolled 26 healthy subjects, half fast and half slow acetylators based on NAT 2 genotyping and pattern of caffeine metabolism. We examined the effect of acetylator status on pharmacokinetics (PK) of single ascending and multiple (QID) oral doses of amifampridine phosphate. Results: Subjects9 mean age was 29±11 years; 18(69%) were male and 23(88%) Caucasian. PKc analysis revealed that slow acetylators had 3-4x higher plasma drug levels than fast acetylators. After a single 20 mg dose, C max was 56.7 ± 16.1 ng/ml in slow vs 16.2 ± 4.56 ng/ml in fast acetylators (p 0-inf was 142 ± 32.1 ng-h/ml in slow vs 24.7 ± 2.47 ng-h/ml in fast acetylators ( p Conclusions: This is the first clinical study of the pharmacogenomics of amifampridine. Polymorphisms in the N-acetyltransferase system create 3-4-fold differences in plasma levels and alter the frequency of adverse events. Supported by: BioMarin Pharmaceuticals Inc. Disclosure: Dr. Haroldsen has received personal compensation for activities with BioMarin Pharmaceutical as an employee.Dr. Haroldsen has received research support from BioMarin Pharmaceutical Inc. Dr. Garovoy has received research support from BioMarin Pharmaceuticals Inc.
Real-World Normal Map Capture for Nearly Flat Reflective Surfaces (2013)

Although specular objects have gained interest in recent years, virtually no approaches exist for marker less reconstruction of reflective scenes in the wild. In this work, we present a practical approach to capturing normal maps in real-world scenes using video only. We focus on nearly planar surfaces such as windows, facades from glass or metal, or frames, screens and other indoor objects and show how normal maps of these can be obtained without the use of an artificial calibration object. Rather, we track the reflections of real-world straight lines, while moving with a hand-held or vehicle-mounted camera in front of the object. In contrast to error-prone local edge tracking, we obtain the reflections by a robust, global segmentation technique of an ortho-rectified 3D video cube that also naturally allows efficient user interaction. Then, at each point of the reflective surface, the resulting 2D-curve to 3D-line correspondence provides a novel quadratic constraint on the local surface normal. This allows to globally solve for the shape by integrability and smoothness constraints and easily supports the usage of multiple lines. We demonstrate the technique on several objects and facades.
Carbonyl Derivatives of Chloride-Dimethyl Sulfoxide-Ruthenium(III) Complexes: Synthesis, Crystal Structure, and Reactivity of [(DMSO)2H][trans-RuCl4(DMSO-O)(CO)] and mer,cis-RuCl3(DMSO-O)2(CO) (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbonyl Derivatives of Chloride-Dimethyl Sulfoxide-Ruthenium(III) Complexes: Synthesis, Crystal Structure, and Reactivity of [(DMSO)2H][trans-RuCl4(DMSO-O)(CO)] and mer,cis-RuCl3(DMSO-O)2(CO)E. Alessio, M. Bolle, B. Milani, G. Mestroni, P. Faleschini, S. Geremia, and M. CalligarisCite this: Inorg. Chem. 1995, 34, 19, 4716–4721Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/ic00123a003https://doi.org/10.1021/ic00123a003research-articleACS PublicationsRequest reuse permissionsArticle Views513Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Non-invasive imaging techniques in the diagnosis of skin diseases (2011)

Available non-invasive imaging techniques in dermatology show substantial differences concerning their limitations and opportunities, potential clinical applicability and practicability. Also, the current data available differ largely between these methods due to different levels of experience in research and clinical application. Future research will not only aim at improving current technical limitations, but also investigate the potential synergistic effects of combining two or more techniques in order to enhance their diagnostic impact.