gebhart/patrepeval huggingface dataset:
C‐reactive protein in acute viral infections (1981)

A sensitive solid-phase enzyme immunoassay procedure was used to determine the concentrations of C-reactive protein (CPR) in the acute and convalescent phase sera of patients with verified rubella, herpes simplex, cytomegalo, influenza A or B, enterovirus, or mycoplasma infection. In all infection groups about 90% (80% for influenza) elevated CRP values were observed in the acute phase sera (mean values in the different groups 16-57 micrograms/ml), the highest values exceeding or approaching 100 micrograms/ml. The serum CRP values were highest in all groups before the specific serum antibodies were detectable and decreased approaching the upper limit or normal controls (2 microgram/ml) within 2 weeks. Notable individual variation in the CRP production was seen. We conclude tha serum CRP determination should not be used as a reliable criterion to distinguish bacterial and viral infections.
Noise Estimation from a Single Image (2006)

In order to work well, many computer vision algorithms require that their parameters be adjusted according to the image noise level, making it an important quantity to estimate. We show how to estimate an upper bound on the noise level from a single image based on a piecewise smooth image prior model and measured CCD camera response functions. We also learn the space of noise level functions how noise level changes with respect to brightness and use Bayesian MAP inference to infer the noise level function from a single image. We illustrate the utility of this noise estimation for two algorithms: edge detection and featurepreserving smoothing through bilateral filtering. For a variety of different noise levels, we obtain good results for both these algorithms with no user-specified inputs.
Patterns of Amino Acids near Signal‐Sequence Cleavage Sites (1983)

According to the signal hypothesis, a signal sequence, once having initiated export of a growing protein chain across the rough endoplasmic reticulum, is cleaved from the mature protein at a specific site. It has long been known that some part of the cleavage specificity resides in the last residue of the signal sequence, which invariably is one with a small, uncharged side-chain, but no further specific patterns of amino acids near the point of cleavage have been discovered so far. In this paper, some such patterns, based on a sample of 78 eukaryotic signal sequences, are presented and discussed, and a first attempt at formulating rules for the prediction of cleavage sites is made.
Scheduling algorithms for multihop radio networks (1993)

Algorithms for transmission scheduling in multihop broadcast radio networks are presented. Both link scheduling and broadcast scheduling are considered. In each instance, scheduling algorithms are given that improve upon existing algorithms both theoretically and experimentally. It is shown that tree networks can be scheduled optimally and that arbitrary networks can be scheduled so that the schedule is bounded by a length that is proportional to a function of the network thickness times the optimum. Previous algorithms could guarantee only that the schedules were bounded by a length no worse than the maximum node degree times optimum. Since the thickness is typically several orders of magnitude less than the maximum node degree, the algorithms presented represent a considerable theoretical improvement. Experimentally, a realistic model of a radio network is given and the performance of the new algorithms is studied. These results show that, for both types of scheduling, the new algorithms (experimentally) perform consistently better than earlier methods.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The i860TM 64-bit supercomputing microprocessor (1989)

The Intel i860TM processor is a RISC-based microprocessor incorporating a RISC core with memory management, a floating point unit, and caches on a single chip. The 1,000,000 transistors allow a single chip implementation with highly optimized interunit communication and wide internal data buses. The parallelism and pipelining between the execution units, and the innovative cache management techniques are under explicit control of software. Vectorizable applications can use the pipelined adder and multiplier units to achieve up to 80 Mflops at 40 Mhz for the inner loops of common calculations. Special instructions allow using the data cache as a flexible vector register and support high data bandwidth from main memory. Finally, to support visualization of data, special hardware for 3D graphics is included.
The Invariance of Characteristic Current Densities in Nanoscale MOSFETs and Its Impact on Algorithmic Design Methodologies and Design Porting of Si(Ge) (Bi)CMOS High-Speed Building Blocks (2006)

This paper provides evidence that, as a result of constant-field scaling, the peak f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</sub> (approx. 0.3 mA/mum), peak f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">MAX </sub> (approx. 0.2 mA/mum), and optimum noise figure NF <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">MIN</sub> (approx. 0.15 mA/mum) current densities of Si and SOI n-channel MOSFETs are largely unchanged over technology nodes and foundries. It is demonstrated that the characteristic current densities also remain invariant for the most common circuit topologies such as MOSFET cascodes, MOS-SiGe HBT cascodes, current-mode logic (CML) gates, and nMOS transimpedance amplifiers (TIAs) with active pMOSFET loads. As a consequence, it is proposed that constant current-density biasing schemes be applied to MOSFET analog/mixed-signal/RF and high-speed digital circuit design. This will alleviate the problem of ever-diminishing effective gate voltages as CMOS is scaled below 90 nm, and will reduce the impact of statistical process variation, temperature and bias current variation on circuit performance. The second half of the paper illustrates that constant current-density biasing allows for the porting of SiGe BiCMOS cascode operational amplifiers, low-noise CMOS TIAs, and MOS-CML and BiCMOS-CML logic gates and output drivers between technology nodes and foundries, and even from bulk CMOS to SOI processes, with little or no redesign. Examples are provided of several record-setting circuits such as: 1) SiGe BiCMOS operational amplifiers with up to 37-GHz unity gain bandwidth; 2) a 2.5-V SiGe BiCMOS high-speed logic chip set consisting of 49-GHz retimer, 40-GHz TIAs, 80-GHz output driver with pre-emphasis and output swing control; and 3) 1-V 90-nm bulk and SOI CMOS TIAs with over 26-GHz bandwidth, less than 8-dB noise figure and operating at data rates up to 38.8 Gb/s. Such building blocks are required for the next generation of low-power 40-80 Gb/s wireline transceivers
Galactofuranose containing molecules inAspergillus fumigatus (2008)

Galactofuranose is a major carbohydrate in Aspergillus fumigatus. It became famous in medical mycology as being part of the galactomannan which was shown 30 years ago to be the major antigen circulating in the body fluid of patients suffering from invasive aspergillosis. Four different molecules contain galactofuranose in A. fumigatus: (i) the galactomannan present in the alkali soluble and insoluble fraction of the cell wall (ii) N- and O glycan moieties of secreted glycoproteins (iii) a GPI- anchored lipophosphogalactomannan and (iv) several sphingolipids also anchored to the membrane by an inositol phosphoceramide.
A method for counting PCR template molecules with application to next-generation sequencing (2011)

Amplification by polymerase chain reaction is often used in the preparation of template DNA molecules for next-generation sequencing. Amplification increases the number of available molecules for sequencing but changes the representation of the template molecules in the amplified product and introduces random errors. Such changes in representation hinder applications requiring accurate quantification of template molecules, such as allele calling or estimation of microbial diversity. We present a simple method to count the number of template molecules using degenerate bases and show that it improves genotyping accuracy and removes noise from PCR amplification. This method can be easily added to existing DNA library preparation techniques and can improve the accuracy of variant calling.
Safety and tolerability of intracerebroventricular PDGF-BB in Parkinson’s disease patients (2015)

BACKGROUND. Recombinant human PDGF-BB (rhPDGF-BB) reduces Parkinsonian symptoms and increases dopamine transporter (DAT) binding in several animal models of Parkinson's disease (PD). Effects of rhPDGF-BB are the result of proliferation of ventricular wall progenitor cells and reversed by blocking mitosis. Based on these restorative effects, we assessed the safety and tolerability of intracerebroventricular (i.c.v.) rhPDGF-BB administration in individuals with PD. METHODS. We conducted a double-blind, randomized, placebo-controlled phase I/IIa study at two clinical centers in Sweden. Twelve patients with moderate PD received rhPDGF-BB via an implanted drug infusion pump and an investigational i.c.v. catheter. Patients were assigned to a dose cohort (0.2, 1.5, or 5 μg rhPDGF-BB per day) and then randomized to active treatment or placebo (3:1) for a 12-day treatment period. The primary objective was to assess safety and tolerability of i.c.v.-delivered rhPDGF-BB. Secondary outcome assessments included several clinical rating scales and changes in DAT binding. The follow-up period was 85 days. RESULTS. All patients completed the study. There were no unresolved adverse events. Serious adverse events occurred in three patients; however, these were unrelated to rhPDGF-BB administration. Secondary outcome parameters did not show dose-dependent changes in clinical rating scales, but there was a positive effect on DAT binding in the right putamen. CONCLUSION. At all doses tested, i.c.v. administration of rhPDGF-BB was well tolerated. Results support further clinical development of rhPDGF-BB for patients with PD. TRIAL REGISTRATION. Clinical Trials.gov NCT00866502. FUNDING. Newron Sweden AB (former NeuroNova AB) and Swedish Governmental Agency for Innovation Systems (VINNOVA).
Health effects of climate change in the UK – current evidence and research gaps (2013)

Scientific consensus generally indicates that climate change is likely to cause a range of direct and indirect effects on human health in developing and developed countries. We reviewed the scientific evidence on the health effects of climate change in the UK in the light of the 2009 UK Climate Projections. We focused on the effects of temperature and heatwaves, floods, air pollution, aeroallergens, vector-borne diseases, airborne, water and food-borne diseases, and UV radiation exposure on public health. Literature reviews were conducted on these topics to identify climate-sensitive health outcomes, population exposure patterns, exposure-response relationships (where possible), and vulnerable populations and areas in the UK. Quantitative health impact assessment methods were used to estimate the effect of current and future temperatures and air pollution (ground-level ozone) on population health under a range of scenarios. In addition, the impacts of climate change in the indoor environment, and the trade-offs between indoor air quality and energy efficiency measures were discussed. Furthermore, health co-benefits from policies to reduce greenhouse gas emissions were illustrated with examples from the household energy, urban transport, electricity generation, and food and agriculture sectors. Despite uncertainties in the analysis, this paper provides strong evidence indicating that climate change is likely to act as a risk modifier aggravating existing public health problems (heat and flood related deaths, air pollution, allergies, mental health, health inequalities, etc.) in the UK. Research gaps in epidemiological evidence, environmental exposure assessment, disease surveillance, and evaluation of public health interventions (e.g. early warning systems for heatwaves, air pollution, pollen and floods) will be identified and discussed in this context.
Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine (2004)

The probiotic drug E coli Nissle 1917 shows efficacy and safety in maintaining remission equivalent to the gold standard mesalazine in patients with ulcerative colitis. The effectiveness of probiotic treatment further underlines the pathogenetic significance of the enteric flora.
Development of an Automated Computer-Controlled Islet Isolation System (1997)

Before clinical islet transplantation can become an effective and reliable treatment for type 1 diabetic patients, there must be significant improvements in the methods employed for the isolation of islets of Langerhans. We have developed an automated cell extraction system (ACES), which allows computer control of the isolation process. As well, it incorporates a novel method of recombining dissociated pancreatic tissue. Following initial system design and testing to determine the optimal system configuration, a series of 12 consecutive canine islet isolations were performed. Pancreases were perfused with collagenase via the duct and dissociated and recombined using either the standard Ricordi-based protocol (group 1, n = 6) or dissociated and recombined using the ACES system (group 2, n = 6). A total of 90.8 ± 21 x 10 3 islet equivalents (IE) (mean ± SEM) were recovered in group 1 vs. 99 ± 14 x 10 3 IE in group 2 (p = NS, student unpaired t-test). Following Ficoll purification the recovery was 56.2 ± 14 x 10 3 IE for group 1 vs. 54.7 ± 11 x 10 3 IE for group 2 (p = NS). Viability was equivalent with an 8.6-fold increase in insulin secretion for group 1 and an 8.8-fold increase for group 2 when the islets were exposed to high glucose solution supplemented with IBMX (3-isobutyl-1-methylxanthine) during static incubation. In vivo function was equivalent following transplantation of 2000 IE under the kidney capsule of alloxan-induced diabetic nude mice with five of six and five of seven mice surviving long-term (&gt;50 days posttransplant) (groups 1 and 2, respectively). This data shows that an entirely automated pancreatic islet extraction system can result in effective canine islet recovery without compromising islet yields and viability. The ACES system has several ad van tages over the standard isolation protocol. These include: 1) computer control and monitoring over all phases of the isolation, 2) a single-use sterile disposable tubing set, and 3) a novel method of tissue recombination. Copyright © 1997 Elsevier Science Inc.
Point mutations in the abl SH2 domain coordinately impair phosphotyrosine binding in vitro and transforming activity in vivo. (1992)

We have constructed a series of point mutations in the highly conserved FLVRES motif of the src homology 2 (SH2) domain of the abl tyrosine kinase. Mutant SH2 domains were expressed in bacteria, and their ability to bind to tyrosine-phosphorylated proteins was examined in vitro. Three mutants were greatly reduced in their ability to bind both phosphotyrosine itself and tyrosine-phosphorylated cellular proteins. All of the mutants that retained activity bound to the same set of tyrosine-phosphorylated proteins as did the wild type, suggesting that binding specificity was unaffected. These results implicate the FLVRES motif in direct binding to phosphotyrosine. When the mutant SH2 domains were inserted into an activated abl kinase and expressed in murine fibroblasts, decreased in vitro phosphotyrosine binding correlated with decreased transforming ability. This finding implies that SH2-phosphotyrosine interactions are involved in transmission of positive growth signals by the nonreceptor tyrosine kinases, most likely via the assembly of multiprotein complexes with other tyrosine-phosphorylated proteins.
Bovine Papilloma Virus Contains an Activator of Gene Expression at the Distal End of the Early Transcription Unit (1983)

Bovine papilloma virus (BPV) contains a cis-acting DNA element which can enhance transcription of distal promoters. Utilizing both direct and indirect transient transfection assays, we showed that a 59-base-pair DNA sequence from the BPV genome could activate the simian virus 40 promoter from distances exceeding 2.5 kilobases and in an orientation-independent manner. In contrast to the promoter 5'-proximal localization of other known viral activators, this element was located immediately 3' to the early polyadenylation signal in the BPV genome. Deletion of these sequences from the BPV genome inactivated the transforming ability of BPV recombinant plasmids. Orientation-independent reinsertion of this 59-base-pair sequence, or alternatively of activator DNA sequences from simian virus 40 or polyoma virus, restored the transforming activity of the BPV recombinant plasmids. Furthermore, the stable transformation frequency of the herpes simplex virus type 1 thymidine kinase gene was enhanced when linked to restriction fragments of BPV DNA which included the defined activator element. This enhancement was orientation independent with respect to the thymidine kinase promoter. The enhancement also appeared to be unrelated to the establishment of the recombinant plasmids as episomes, since in transformed cells these sequences are found linked to high-molecular-weight DNA. We propose that the enhancement of stable transformation frequencies and the activation of transcription units are in this case alternate manifestations of the same biochemical events.
[Utility of myocardial scintigraphy with Tc-99m methoxy-isobutyl-isonitrile in the diagnosis of coronary disease]. (1990)

We have studied 24 patients (21 males and 3 females) with coronary artery disease. Stress and rest myocardial scintigraphy was performed in all cases with 15-20 mCi of Tc99m methoxy-isobutyl-isonitrile. The examination was done in 2 sessions. Usually the stress scan was obtained and within 2-4 days the rest study was obtained. All patients underwent selective coronary arteriography within a week of the scintigraphy and, in 13 cases, rest radiologic ventriculography was also performed. In 5 patients coronary arteriography and myocardial scintigraphy were normal. In 19 cases the coronary arteriography showed lesions, the myocardial scintigraphy showed ischemia or necrosis in 17 of them and was normal in two. To localize the pathologic vessel the myocardium was divided into 3 segments and segmentary analysis localized the abnormal coronary artery in 51 out of 72 cases. The best availability of the new myocardial agent and the results obtained make it a good tracer for myocardial studies.
Unusual behavior of vaporized magnesium under low pressure conditions (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnusual behavior of vaporized magnesium under low pressure conditionsL. B. Knight Jr., R. D. Brittain, M. Duncan, and C. H. JoynerCite this: J. Phys. Chem. 1975, 79, 12, 1183–1190Publication Date (Print):June 1, 1975Publication History Published online1 May 2002Published inissue 1 June 1975https://pubs.acs.org/doi/10.1021/j100579a008https://doi.org/10.1021/j100579a008research-articleACS PublicationsRequest reuse permissionsArticle Views249Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Molecular identification of aspartate N-acetyltransferase and its mutation in hypoacetylaspartia (2009)

The brain-specific compound NAA (N-acetylaspartate) occurs almost exclusively in neurons, where its concentration reaches approx. 20 mM. Its abundance is determined in patients by MRS (magnetic resonance spectroscopy) to assess neuronal density and health. The molecular identity of the NAT (N-acetyltransferase) that catalyses NAA synthesis has remained unknown, because the enzyme is membrane-bound and difficult to purify. Database searches indicated that among putative NATs (i.e. proteins homologous with known NATs, but with uncharacterized catalytic activity) encoded by the human and mouse genomes two were almost exclusively expressed in brain, NAT8L and NAT14. Transfection studies in HEK-293T [human embryonic kidney-293 cells expressing the large T-antigen of SV40 (simian virus 40)] indicated that NAT8L, but not NAT14, catalysed the synthesis of NAA from L-aspartate and acetyl-CoA. The specificity of NAT8L, its Km for aspartate and its sensitivity to detergents are similar to those described for brain Asp-NAT. Confocal microscopy analysis of CHO (Chinese-hamster ovary) cells and neurons expressing recombinant NAT8L indicates that it is associated with the ER (endoplasmic reticulum), but not with mitochondria. A mutation search in the NAT8L gene of the only patient known to be deficient in NAA disclosed the presence of a homozygous 19 bp deletion, resulting in a change in reading frame and the absence of production of a functional protein. We conclude that NAT8L, a neuron-specific protein, is responsible for NAA synthesis and is mutated in primary NAA deficiency (hypoacetylaspartia). The molecular identification of this enzyme will lead to new perspectives in the clarification of the function of this most abundant amino acid derivative in neurons and for the diagnosis of hypoacetylaspartia in other patients.
Tetranucleotide repeat polymorphism at the human thyroid peroxidase (hTPO) locus (1992)

Journal Article Tetranucleotide repeat polymorphism at the human thyroid peroxidase (hTPO) locus Get access R. Anker, R. Anker Department of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar T. Steinbrueck, T. Steinbrueck Department of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Donis-Keller H. Donis-Keller * Department of Genetics, Washington University School of MedicineSt Louis, MO 63110, USA *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Human Molecular Genetics, Volume 1, Issue 2, May 1992, Page 137, https://doi.org/10.1093/hmg/1.2.137 Published: 01 May 1992