gebhart/patrepeval huggingface dataset:
Aspirin Does Not Affect the Flow Cytometric Detection of Fibrinogen Binding to, or Release of α-Granules or Lysosomes from, Human Platelets (1994)

1. Aspirin inhibits the conversion of arachidonic acid to thromboxane A2 which reinforces the effects of weak agonists such as ADP in platelets. 2. In this study the effect of aspirin (300 mg/day) on platelet agonist response was measured by whole blood flow cytometry of unfixed blood samples from normal subjects (n = 10), an assay that investigates aggregation-independent changes in the platelet. 3. Fibrinogen binding to unstimulated platelets or to platelets stimulated with ADP or thrombin was unaffected by aspirin. 4. Under the conditions of this assay, platelets undergo a partial degranulation of alpha-granules and lysosomes (evidenced by expression of P-selectin and CD63, respectively) in response to ADP, and full degranulation in response to thrombin. P-selectin expression was paralleled by release of beta-thromboglobulin. None of these events was affected by aspirin. 5. Thromboxane formation was totally prevented by the aspirin treatment, as shown by Born aggregometry in which the platelet aggregatory response to arachidonic acid was abolished and secondary aggregation by ADP was inhibited. 6. The flow cytometric assay can therefore be used to investigate platelets in patients, regardless of aspirin therapy. 7. These findings suggest that platelet fibrinogen binding and the release of platelet alpha-granule and lysosomal contents, in response to stimulation with physiological agonists, can continue in patients despite aspirin therapy. This may help to explain why aspirin is only partially effective in preventing thrombotic events.
Expanding the Rule Set of DNA Circuitry with Associative Toehold Activation (2011)

Toehold-mediated strand displacement has proven extremely powerful in programming enzyme-free DNA circuits and DNA nanomachines. To achieve multistep, autonomous, and complex behaviors, toeholds must be initially inactivated by hybridizing to inhibitor strands or domains and then relieved from inactivation in a programmed, timed manner. Although powerful and reasonably robust, this strategy has several drawbacks that limit the architecture of DNA circuits. For example, the combination between toeholds and branch migration (BM) domains is 'hard wired' during DNA synthesis thus cannot be created or changed during the execution of DNA circuits. To solve this problem, I propose a strategy called 'associative toehold activation', where the toeholds and BM domains are connected via hybridization of auxiliary domains during the execution of DNA circuits. Bulged thymidines that stabilize DNA three-way junctions substantially accelerate strand displacement reactions in this scheme, allowing fast strand displacement initiated by reversible toehold binding. To demonstrate the versatility of the scheme, I show (1) run-time combination of toeholds and BM domains, (2) run-time recombination of toeholds and BM domains, which results in a novel operation 'toehold switching', and (3) design of a simple conformational self-replicator.
Eliminate Reactive Power and Increase System Efficiency of Isolated Bidirectional Dual-Active-Bridge DC–DC Converters Using Novel Dual-Phase-Shift Control (2008)

This paper proposes a novel dual-phase-shift (DPS) control strategy for a dual-active-bridge isolated bidirectional DC-DC converter. The proposed DPS control consists of a phase shift between the primary and secondary voltages of the isolation transformer, and a phase shift between the gate signals of the diagonal switches of each H-bridge. Simulation on a 600-V/5-kW prototype shows that the DPS control has excellent dynamic and static performance compared to the traditional phase-shift control (single phase shift). In this paper, the concept of ldquoreactive powerrdquo is defined, and the corresponding equations are derived for isolated bidirectional DC-DC converters. It is shown that the reactive power in traditional phase-shift control is inherent, and is the main factor contributing to large peak current and large system loss. The DPS control can eliminate reactive power in isolated bidirectional DC-DC converters. In addition, the DPS control can decrease the peak inrush current and steady-state current, improve system efficiency, increase system power capability (by 33%), and minimize the output capacitance as compared to the traditional phase-shift control. The soft-switching range and the influence of short-time-scale factors, such as deadband and system-level safe operation area, are also discussed in detail. Under certain operation conditions, deadband compensation can be implemented easily in the DPS control without a current sensor.
Molecular cloning of the rat adipocyte hormone-sensitive cyclic GMP-inhibited cyclic nucleotide phosphodiesterase. (1993)

Two distinct but related cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI PDE) cDNAs were cloned from rat adipose tissue cDNA libraries. The open reading frame (3324 base pairs) of RcGIP1 encodes 1108 amino acids, including a hydrophobic membrane-associated domain in the NH2-terminal portion and, in the COOH-terminal portion, a putative catalytic domain conserved among all mammalian PDEs which is preceded by a putative regulatory domain that contains three consensus cAMP-dependent protein kinase phosphorylation sites and followed by a hydrophilic COOH-terminal domain. The carboxyl-terminal portion including the conserved domain was expressed as a glutathione S-transferase fusion protein and exhibited cAMP PDE activity which was inhibited by cilostamide, a specific cGI PDE inhibitor. RcGIP1 cDNA hybridizes strongly with RNA from isolated adipocytes, and its mRNA increases dramatically during differentiation of 3T3-L1 adipocytes. The deduced sequence of the second partial cDNA clone (RcGIP2 clone 53B) is highly homologous to the corresponding region of human cardiac cGI PDE cDNA. RcGIP2 cDNA hybridized strongly with rat cardiac tissue RNA and weakly if at all with RNA from rat adipocytes or 3T3-L1 fibroblasts or adipocytes. We suggest that RcGIP1 represents the hormone-sensitive, membrane-associated rat adipocyte cGI PDE and RcGIP2, a cGI PDE from vascular elements in rat adipose tissue.
CORRELATION BETWEEN ATOMIC-SCALE STRUCTURES AND MACROSCOPIC ELECTRICAL PROPERTIES OF METAL-COVERED Si(111) SURFACES (1993)

In this review, we discuss the relation between the atomic-scale structures (atomic arrangements and electronic states) and the macroscopic electrical properties (surface conductance and Schottky barriers) of metal(Ag, Au, or In)-covered Si (111) surfaces. These surfaces have been one of the most intensively investigated systems with the use of a variety of modern surface science techniques, and diversified information at atomic scales has been obtained. The data of reflection high-energy electron diffraction, scanning tunneling microscopy/spectroscopy, photoemission spectroscopies, and others are utilized here for characterizing the structures. Surface conductance and Schottky barriers, on the other hand, have also been the major areas in semiconductor physics for, especially device-oriented, research, but these have rarely been studied in combination with atomic-scale structures. These electrical properties have recently been found to be crucially dependent on the local atomic structures of well-defined surfaces/interfaces. The atomic arrangements and the resulting surface/interface electronic states govern the Fermi-level pinning and band bending which determine the electrical properties of semiconductor surfaces/interfaces.
Caught in the cross fire: p53 in inflammation (2014)

The p53 transcription factor is a major tumor suppressor, whose diverse activities serve to ensure genome stability and inhibit neoplastic processes. In recent years, it is becoming increasingly clear that p53 also plays a broader role in maintaining cellular homeostasis, as well as contributing to tissue homeostasis in a non-cell-autonomous fashion. Chronic inflammation is a potential cancer-promoting condition, and as such is also within the radar of p53, which mounts a multifaceted attempt to prevent the escalation of chronic tissue imbalance into neoplasia. Recent understanding of the p53 pathway and other family members reveals a broad interaction with inflammatory elements such as reactive oxygen and nitrogen species, cytokines, infectious agents and major immune-regulatory pathways like nuclear factor-kappaB. This complex cross talk is highly dependent on p53 status, as different p53 isoforms and p53 mutants can mediate different responses and even promote chronic inflammation and associated cancer, acting in the tumor cells as well as in the stromal and immune compartments.
Improving the Microdialysis Procedure for Electrospray Ionization Mass Spectrometry of Biological Samples (1997)

Significant advances in the area of microdialysis which allowed more effective handling of small volumes (microliters) of samples, more efficient desalting and enhanced mass spectrometric detection sensitivity are described. The previously reported on-line coupling of microdialysis with electrospray ionization (ESI) mass spectrometry has been found to be highly effective; however, direct coupling requires relatively high sample flow rates (∽2 μl min-1) to obtain a stable ESI current compared with the flow rates of newer ESI sources (e.g. ‘microspray,’ 10–100 nl min-1). To circumvent this major limitation imposed by the dimensions of currently available materials, the microdialysis procedure was modified to an off-line mode in order to avoid excessive sample consumption. A more than tenfold decrease in sample consumption was achieved using the off-line mode vs the on-line mode, which resulted in a similar quality spectrum. In addition, several other aspects of the microdialysis approach were altered to improve its performance further: (i) an increase in dialysis temperature was found to increase the desalting efficiency greatly and therefore improve the spectrum quality; (ii) the addition of piperidine and imidazole to the dialysis buffer solution resulted in a reduction of charge states and a further increase in detection sensitivity for DNA and (iii) use of low concentrations (0–2.5 mM NH4OAc) of dialysis buffer shifted the DNA negative ions to higher charge states and produced a nearly tenfold increase in detection sensitivity and a slightly decreased desalting efficiency. Protocols for desalting different samples using microdialysis are discussed. © 1997 by John Wiley & Sons, Ltd.
Experimental findings on gynecologic cell orientation and dynamics for three flow nozzle geometries. (1977)

Gynecologic cell orientation and dynamics in sheath flow for three flow nozzle geometries are studied and compared. Cell orientations are classified into four categories based upon visual inspection of dark field laser stroboscopic photographs of cells in flow. Results are discussed in the context of slit-scan cytofluorometry.
Revising and Validating Spectral Irradiance Reference Standards for Photovoltaic Performance Evaluation (2004)

In 1982, the American Society for Testing and Materials (ASTM) adopted consensus standards for direct-normal and hemispherical (“global”) tilted solar terrestrial spectra (ASTM E891/E892/G159). These standard spectra were intended to evaluate photovoltaic (PV) device performance and other solar-related applications. The International Standards Organization (ISO) and International Electrotechnical Commission (IEC) adopted these spectra as spectral standards ISO 9845-1 and IEC 60904-3. Additional information and more accurately representative spectra are needed by today’s PV community. Modern terrestrial spectral radiation models, knowledge of atmospheric physics, and measured radiometric quantities are applied to develop new reference spectra under consideration by ASTM.
New Clues to Superconductivity (1994)

This article describes experiments being performed to establish the mechanism of high-temperature superconductivity. Described especially are experiments designed to establish the validity of one of two types of theories, phonon-based theories or spin-fluctuation theories. Evidence so far points to spin-fluctuation theories, but is not yet conclusive.
Engineered Human Antibody Constant Domains with Increased Stability (2009)

The immunoglobulin (Ig) constant CH2 domain is critical for antibody effector functions. Isolated CH2 domains are promising as scaffolds for construction of libraries containing diverse binders that could also confer some effector functions. However, previous work has shown that an isolated murine CH2 domain is relatively unstable to thermally induced unfolding. To explore unfolding mechanisms of isolated human CH2 and increase its stability gamma1 CH2 was cloned and a panel of cysteine mutants was constructed. Human gamma1 CH2 unfolded at a higher temperature (T(m) = 54.1 degrees C, as measured by circular dichroism) than that previously reported for a mouse CH2 (41 degrees C). One mutant (m01) was remarkably stable (T(m) = 73.8 degrees C). Similar results were obtained by differential scanning calorimetry. This mutant was also significantly more stable than the wild-type CH2 against urea induced unfolding (50% unfolding at urea concentration of 6.8 m versus 4.2 m). The m01 was highly soluble and monomeric. The existence of the second disulfide bond in m01 and its correct position were demonstrated by mass spectrometry and nuclear magnetic resonance spectroscopy, respectively. The loops were on average more flexible than the framework in both CH2 and m01, and the overall secondary structure was not affected by the additional disulfide bond. These data suggest that a human CH2 domain is relatively stable to unfolding at physiological temperature, and that both CH2 and the highly stable mutant m01 are promising new scaffolds for the development of therapeutics against human diseases.
The controlled homogeneous organic solution polymerization of new hydrophilic cationic exo‐7‐oxanorbornenes via ROMP with RuCl2(PCy3)2CHPh in a novel 2,2,2‐trifluoroethanol/methylenechloride solvent mixture (2007)

Abstract The authors detail herein the synthesis and controlled polymerization of a series of new permanently cationic ammonium exo ‐7‐oxanorbornene derivatives via ROMP, with the first generation Grubbs' catalyst RuCl 2 (PCy 3 ) 2 CHPh, in a novel solvent mixture composed of 1:1 vol/vol 2,2,2‐trifluoroethanol (TFE)/methylene chloride. It is demonstrated that this cosolvent mixture is a convenient reaction medium facilitating the polymerization of hydrophilic substrates by hydrophobic initiators under homogeneous conditions. Homopolymerizations and copolymerizations proceed yielding materials with controlled molecular masses, and narrow molecular mass distributions. It is also demonstrated that this protocol is not limited to the use of TFE as a cosolvent and that additional halogenated alcohols, such as 2,2,2‐trichloroethanol and 1,1,1,3,3,3‐hexafluoroisopropanol are also effective cosolvents for the controlled polymerization of such cationic substrates. Finally, it is demonstrated that the TFE/methylene chloride mixture has no apparent detrimental effect on Grubbs' catalyst. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 2113–2128, 2007
Combined source and channel coding based on multimode coding (2002)

A multimode source/channel coder which can dynamically control the balance of source and channel coding according to the channel quality is introduced. As an example of such a system, a 4.8-kb/s code excited linear predictive coder with three coding modes (A, B1, and B2), each of which has different bit assignments to source and channel coding, is presented. The optimum coding mode is selected in each frame, based on an evaluation of the spectral distortion (SN/sub LAR/) in reproduced speech. The threshold value of SN/sub LAR/ for mode decision is varied according to the channel error rate. Computer simulation shows that 2 dB and 3 dB of SNR/sub seg/ improvement is achieved at a bit error rate of 3*10/sup -3/ and 3*10/sup -2/ respectively, over conventional CELP without channel coding.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Critical Heat Flux (CHF) of Subcooled Flow Boiling of Alumina Nanofluids in a Horizontal Microchannel (2010)

This work investigates subcooled flow boiling of aqueous based alumina nanofluids in 510 μm single microchannels with a focus on the effect of nanoparticles on the critical heat flux. The surface temperature distribution along the pipe, the inlet and outlet pressures and temperatures are measured simultaneously for different concentrations of alumina nanofluids and de-ionized water. To minimize the effect of nanoparticle depositions, all nanofluid experiments are performed on fresh microchannels. The experiment shows an increase of ∼51% in the critical heat flux under very low nanoparticle concentrations (0.1 vol %). Different burnout characteristics are observed between water and nanofluids, as well as different pressure and temperature fluctuations and flow pattern development during the stable boiling period. Detailed observations of the boiling surface show that nanoparticle deposition and a subsequent modification of the boiling surface are common features associated with nanofluids, which should be responsible for the different boiling behaviors of nanofluids.
Studies on Cardiotonic Steroid Analogs. V. : Synthesis of 17&beta;(or &alpha;)-Isoxazolyl and Pyrazolyl-16-methyl-14&beta;(or &alpha;)-androst-5-enes (1970)

In order to examine the cardiotonic and other biological activities, synthesis of steroids with N-heterocyclic ring was carried out. Reaction of 3β-acetoxy-20-ethoxy-21-formylpregna-5, 20-diene (II) with hydroxylamine, hydrazine, methylhydrazine, and phenylhydrazine afforded the corresponding androsten-17-yl isoxazole and pyrazole derivatives (III -VII). In a similar manner, 17β(or α)-isoxazolyl and pyrazolyl-16-methyl-14β(or-α)-androst-5-enes (X-XV, XVIII-XXII) were prepared from the corresponding unsaturated aldehydes having 14, 17-cis configuration (IX, XVII). The position of the pyrazole ring linked to the steroid ring was established on the basis of nuclear magnetic resanace (NMR) spectral data as listed in Table I.
The colored revolution of bioimaging (2006)

This paper provides an overview of the main aspects of modern fluorescence microscopy. It covers the principles of fluorescence and highlights the key discoveries in the history of fluorescence microscopy. The paper also discusses the optics of fluorescence microscopes and examines the various types of detectors. It also discusses the signal and image processing challenges in fluorescence microscopy and highlights some of the present developments and future trends in the field.
Efficient biased sampling for approximate clustering and outlier detection in large data sets (2003)

We investigate the use of biased sampling according to the density of the data set to speed up the operation of general data mining tasks, such as clustering and outlier detection in large multidimensional data sets. In density-biased sampling, the probability that a given point will be included in the sample depends on the local density of the data set. We propose a general technique for density-biased sampling that can factor in user requirements to sample for properties of interest and can be tuned for specific data mining tasks. This allows great flexibility and improved accuracy of the results over simple random sampling. We describe our approach in detail, we analytically evaluate it, and show how it can be optimized for approximate clustering and outlier detection. Finally, we present a thorough experimental evaluation of the proposed method, applying density-biased sampling on real and synthetic data sets, and employing clustering and outlier detection algorithms, thus highlighting the utility of our approach.
Andrological Parameters in Men With High Sperm Counts and Possible Correlation With Age (1990)

Two hundred eighty six human semen samples with sperm counts above 200 million/ml, were divided into 3 groups according to semen volume: (1) less than 1 ml (low), (2) 1 ml-5 ml (normal), and (3) greater than or equal to 6 ml (high). Each group was examined for routine andrological parameters. Also examined was a possible correlation between sperm counts, semen volumes, and ages of patients. The quality of motility (percent and grade) and viability of sperm were generally lowest in specimens with a volume below 1 ml and highest in those of normal volume. Proportions of morphological normality exhibited a similar trend, lacking, however, statistical significance. The mean fructose content of low-volume semen was significantly decreased in comparison with those of normal volume. Patients with low-volume semen and sperm counts above 200 million/ml were older than those with similar sperm counts and normal volume. The mean ages of all patients with high sperm counts of semen were higher than of those with lower sperm counts. It was assumed that in contradiction to specimens with high sperm counts and low volume, those whose volumes range from 1 ml-5 ml should be considered normal with respect to motility, viability, and morphological normality.
Systemic administration of 2‐hydroxypropyl‐β‐cyclodextrin to symptomatic Npc1‐deficient mice slows cholesterol sequestration in the major organs and improves liver function (2014)

In Niemann-Pick type C (NPC) disease, loss-of-function mutations in either NPC1 or NPC2 result in progressive accumulation of unesterified cholesterol (UC) and glycosphingolipids in all organs, leading to neurodegeneration, pulmonary dysfunction and sometimes liver failure. There is no cure for this disorder. Studies using primarily NPC mouse models have shown that systemic administration of 2-hydroxypropyl-β-cyclodextrin (2HPβCD), starting in early neonatal life, diminishes UC accumulation in most organs, slows disease progression and extends lifespan. The key question now is whether delaying the start of 2HPβCD treatment until early adulthood, when the amount of entrapped UC throughout the body is markedly elevated, has any of the benefits found when treatment begins at 7 days of age. In the present study, Npc1(-/-) and Npc1(+/+) mice were given saline or 2HPβCD subcutaneously at 49, 56, 63 and 70 days of age, with measurements of organ weights, liver function tests and tissue cholesterol levels performed at 77 days. In Npc1(-/-) mice, treatment with 2HPβCD from 49 days reduced whole-liver cholesterol content at 77 days from 33.0 ± 1.0 to 9.1 ± 0.5 mg/organ. Comparable improvements were seen in other organs, such as the spleen, and in the animal as a whole. There was a transient increase in biliary cholesterol concentration in Npc1(-/-) mice after 2HPβCD. Plasma alanine aminotransferase and aspartate aminotransferase activities in 77-day-old 2HPβCD-treated Npc1(-/-) mice were reduced compared with saline-treated controls. The lifespan of Npc1(-/-) mice given 2HPβCD marginally exceeded that of the saline-treated controls (99 ± 1.1 vs 94 ± 1.4 days, respectively; P < 0.05). Thus, 2HPβCD is effective in mobilizing entrapped cholesterol in late-stage NPC disease leading to improved liver function.
Grain Overload in Cattle and Sheep: Changes in Microbial Populations in the Cecum and Rumen (1975)

Samples from the rumen and cecum of cattle and sheep were cultured to determine changes in microbial populations resulting from overfeeding with grain. Before the animals were overfed, the predominant organisms from both sides were those that grew anaerobically on a relatively nonselective ruminal fluid medium and would not grow on selective mediums designed to culture lactobacilli, streptococci, coliforms, or Clostridium perfringens. By 24 hours after overfeeding, lactic acid bacteria had increased in numbers so that they were the most numerous organisms in both the rumen and the cecum. The concentrations of coliforms and C perfringens also increased after overfeeding and were generally higher in the cecum than in the rumen.