gebhart/patrepeval huggingface dataset:
Stem cell‐based cell therapy in neurological diseases: A review (2009)

Human neurological disorders such as Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis (ALS), Alzheimer's disease, multiple sclerosis (MS), stroke, and spinal cord injury are caused by a loss of neurons and glial cells in the brain or spinal cord. Cell replacement therapy and gene transfer to the diseased or injured brain have provided the basis for the development of potentially powerful new therapeutic strategies for a broad spectrum of human neurological diseases. However, the paucity of suitable cell types for cell replacement therapy in patients suffering from neurological disorders has hampered the development of this promising therapeutic approach. In recent years, neurons and glial cells have successfully been generated from stem cells such as embryonic stem cells, mesenchymal stem cells, and neural stem cells, and extensive efforts by investigators to develop stem cell-based brain transplantation therapies have been carried out. We review here notable experimental and preclinical studies previously published involving stem cell-based cell and gene therapies for Parkinson's disease, Huntington's disease, ALS, Alzheimer's disease, MS, stroke, spinal cord injury, brain tumor, and lysosomal storage diseases and discuss the future prospects for stem cell therapy of neurological disorders in the clinical setting. There are still many obstacles to be overcome before clinical application of cell therapy in neurological disease patients is adopted: 1) it is still uncertain what kind of stem cells would be an ideal source for cellular grafts, and 2) the mechanism by which transplantation of stem cells leads to an enhanced functional recovery and structural reorganization must to be better understood. Steady and solid progress in stem cell research in both basic and preclinical settings should support the hope for development of stem cell-based cell therapies for neurological diseases.
Treatment of patients with progressive unresectable metastatic melanoma with a heterologous polyvalent melanoma whole cell vaccine (2003)

Unresectable metastatic melanoma has no elective treatment. Neither chemotherapy, intravenous IL-2 nor biochemotherapy clearly improves the overall survival. Recent assays with therapeutic vaccines have been recently yielded promising results. Here, we describe the application, clinical tolerance and antitumoural activity of a heterologous polyvalent melanoma whole cell vaccine in patients with metastatic melanoma. Twenty-eight AJCC stage III/IV melanoma patients with progressive unresectable metastatic disease were treated with our heterologous polyvalent melanoma whole cell vaccine between July 1, 1998 and July 1, 2002. All patients had already been unsuccessfully treated with high doses of IFN-alpha2 and/or polychemotherapy and/or biochemotherapy and/or perfusion of extremities, or could not receive other treatments due to their age or underlying illness. Twenty-three were assessable. The vaccine was constituted by 10 melanoma cell lines, derived from primary, lymph node and metastatic melanomas. Prior to intradermal inoculation, the cells were irradiated and mixed with BCG, and 50% were treated with DNFB. After a median follow-up of 19 months, 26% of patients responded: 3 CR (18, 16+, and 26+ months), 2 PR (8 and 22 months) and 1 MR (36+ months). The median survival of the whole group was 20.2 months. None of the 28 patients initially included in the study presented significant toxicity. This vaccination program had specific antitumoural activity in advanced metastatic melanoma patients and was well tolerated. The clinical responses and the median survival of our group of patients, together with the low toxicity of our polyvalent vaccine, suggest that this approach could be applied to earlier metastatic melanoma patients.
P3‐074: Cardiac function is related to maladaptive brain aging in mild cognitive impairment: Preliminary results (2009)

Our prior research suggests diminished cardiac output is related to neuropsychological and neuroanatomic abnormalities in patients with cardiovascular disease (CVD). It is not yet known if these relations are associated with maladaptive brain aging in older adults with mild cognitive impairment (MCI). The current study relates cardiac function (cardiac output and ejection fraction) to early cognitive and neuroimaging markers of cerebrovascular and Alzheimer's disease (AD) pathology among individuals with MCI. 3T cardiac and brain MRI and neuropsychological data were collected on 10 MCI participants free from clinical stroke (62-84 years, 75±7; 46% women). Cardiac output and ejection fraction, determined by cardiac MRI, were related to neuropsychological and neuroimaging markers of vascular (executive functioning, fractional anisotropy) and AD pathology (learning and memory, hippocampal volume). Partial correlations (pr), adjusting for systolic blood pressure and body surface area, suggest that cardiac output is significantly associated with Digit Symbol (pr=0.72, p=0.04), California Verbal Learning Test Long Delay Recall (pr=0.95, p=0.001), Biber Figure Learning Test Trials 1-5 Recall (pr=0.13, p=0.04), and hippocampal volume (pr=0.76, p=0.04). Partial correlations, adjusting for systolic blood pressure, suggest that ejection fraction is significantly associated with Color-Word Interference (pr=-0.66, p=0.05). In all cases, as cardiac function decreased, the integrity of the brain aging phenotype also decreased. Our preliminary data based on a very small sample of MCI patients suggest that cardiac function is related to brain aging phenotypes indicative of early Alzheimer's disease (memory, hippocampal volume) and cerebrovascular changes (executive functioning). Additional cross-sectional data collection and longitudinal follow-up up this cohort will provide essential information in determining if cardiac function is associated with accelerating maladaptive brain aging among older adults at risk for cognitive decline. The successful completion of this research will increase understanding about the complex interrelations between subclinical impairments in cardiac function and cognitive progression in MCI.
University of Miami Division of Clinical Pharmacology Therapeutic Rounds (1999)

Gastroparesis, defined as delayed gastric emptying because of abnormal gastric motility in the absence of mechanical outlet obstruction, is a common problem causing significant morbidity. Although many cases are caused by diabetes, more than 90 different conditions are known to interfere with normal gastric motor function (Scand J Gastroenterol 1995;30[suppl]:7-16). Patients may present with nausea, vomiting, heartburn, early satiety, or postprandial pain. The current gold standard for quantifying gastric emptying is nuclear scintigraphy. The main goal of treatment is to improve patient comfort by accelerating the rate of gastric emptying, which may be achieved through dietary changes and the use of prokinetic agents. In rare instances, relief can only be obtained with surgical intervention. This report reviews the pathophysiology, clinical presentation, evaluation, and treatment of patients with gastroparesis, an understanding of which will lead to more effective patient care.
Development of a genetically marked recombinant rinderpest vaccine expressing green fluorescent protein (2000)

In order to effectively control and eliminate rinderpest, a method is required to allow serological differentiation between animals that have been vaccinated and those which have recovered from natural infection. One way of doing this would be to engineer the normal vaccine to produce a genetically marked rinderpest virus (RPV) vaccine. We constructed two modified cDNA clones of the RPV RBOK vaccine strain with the coding sequence of the green fluorescent protein (GFP) gene inserted as a potential genetic marker. RPVINS-GFP virus was designed to produce independent and high level expression of GFP inside infected cells, whilst the GFP expressed by RPVSIG-GFP virus was designed to be efficiently secreted. Infectious recombinant virus was rescued in cell culture from both constructs. The effectiveness of these viruses in stimulating protective immunity and antibody responses to the marker protein was tested by vaccination of cattle and goats. All of the vaccinated animals were completely protected when challenged with virulent virus: RPV in cattle or peste-des-petits ruminants virus in the goats. ELISA showed that all of the animals produced good levels of anti-RPV antibodies. Three of the four cattle and the two goats vaccinated with RPVSIG-GFP produced detectable levels of anti-GFP antibodies. In contrast, no anti-GFP antibodies were produced in the four cattle and two goats vaccinated with RPVINS-GFP. Therefore, secretion of the GFP marker protein was absolutely required to elicit an effective humoral antibody response to the marker protein.
Harmonic phase conjugation in liquid suspensions of microparticles via higher-order gratings (1985)

We demonstrate that a liquid suspension of microparticles can be utilized to generate harmonic phase conjugate waves. This phenomenon involves the formation of higher-order particle gratings via electrostrictive forces generated by the low-frequency pump and probe fields. In turn, such gratings can coherently scatter high-frequency pump radiation, oscillating at a harmonic of the probe wave to form a conjugate wave oscillating at the same frequency.
Time series analysis and sleep research (1974)

The characterization of biological data during sleepwaking state changes requires the utilization of time series and point process techniques of analysis. The time series procedures that have been found useful in describing biological activity during sleep include frequency domain techniques, such as the autospectrum, digital filtering, complex demodulation, coherence, and cross-spectrum calculations, as well as time domain procedures, such as autocorrelation and matched filtering. The point process techniques include interval distributions, intensity functions, serial correlations, and cross-intensity functions. In addition, applications of frequency domain techniques such as the interval spectrum and spectrum of counts have been implemented for point process data.
Chemical piezoelectric sensor and sensor array characterization (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical piezoelectric sensor and sensor array characterizationW. Patrick. Carey and Bruce R. KowalskiCite this: Anal. Chem. 1986, 58, 14, 3077–3084Publication Date (Print):December 1, 1986Publication History Published online1 May 2002Published inissue 1 December 1986https://pubs.acs.org/doi/10.1021/ac00127a037https://doi.org/10.1021/ac00127a037research-articleACS PublicationsRequest reuse permissionsArticle Views776Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Visible light emission from semiconducting polymer diodes (1991)

We report visible light emission from Shottky diodes made from semiconducting polymers, confirming the discovery by the Cambridge group [Nature 347, 539 (1990)]. Our results demonstrate that light-emitting diodes can be fabricated by casting the polymer film from solution with no subsequent processing or heat treatment required. Electrical characterization reveals diode behavior with rectification ratios greater than 104. We propose that tunneling of electrons from the recitifying metal contact into the gap states of the positive polaron majority carriers dominates current flow and provides the mechanism for light emission.
A simple assay for chitin application to sediment trap samples from the subarctic Pacific (1990)

Chitin is thought to be abundant in marine environments, but examination of the role of chitin in nutrient cycling has been hampered by the lack of an adequate assay for measuring concentrations at ambient levels. We developed a simple assay using the lectin, wheat germ agglutinin (WGA), which has an affinity for the N-acetylglucosamine (NAG) residues found in chitin. The specificity of the assay was confirmed by enzymatic hydrolysis with chitinase and competitive inhibition with chltotriose. The lectin bound specifically to chitin even when samples contained high concentrations of cellulose, clay, and bacteria. Concentrations of suspended chitin were 4 to 21 pg 1-' in Delaware Bay, USA, and 4 to 10 pg 1-' in the subarctic Pacific. The assay was also applied to sediment trap samples collected in the subarctic Pacific. We found that the chitin flux accounted for less than 1 % of carbon and nitrogen fluxes above 500 m. Using fluorescently-labelled WGA and epifluorescence microscopy, we were able to differentiate detritus, zooplankton fecal pellets and possibly fungi from nonchltinous particles.
The human natural anti-Gal IgG. III. The subtlety of immune tolerance in man as demonstrated by crossreactivity between natural anti-Gal and anti-B antibodies. (1987)

A well-defined antigen/antibody system was used to evaluate the effect of immune tolerance on the spectrum of specificities of natural antibodies. The antibody used in this study, anti-Gal, is a naturally occurring, polyclonal IgG that constitutes 1% of the circulating IgG in humans. We have previously shown that anti-Gal, purified from AB sera, specifically interacts with glycosphingolipids bearing a Gal alpha 1----3Gal epitope, but not with the closely related B antigen in which the penultimate galactose of the Gal alpha 1----3Gal epitope is fucosylated Gal alpha 1----3(Fuc alpha 1----2)Gal. This narrow specificity was assumed to be the result of an effective immune tolerance mechanism that prevents the expression of antibody clones that can recognize both the Gal alpha 1----3Gal and the self B epitopes. If the assumption that immune tolerance determines the range of anti-Gal specificity is correct, then anti-Gal from individuals lacking the B antigen (A and O blood types) would be expected to interact with both Gal alpha 1----3Gal and Gal alpha 1----3(Fuc alpha 1----2)Gal epitopes. In this study, anti-Gal from the serum of individuals of various blood types was purified by affinity chromatography on Gal alpha 1----3Gal adsorbent and tested for its reaction with the B antigen. Whereas anti-Gal from AB and B individuals only reacted with Gal alpha 1----3Gal epitopes, anti-Gal from A and O individuals reacted with both Gal alpha 1----3Gal and B epitopes. Furthermore, it was determined that the majority of anti-B reactivity in A and O individuals is in fact anti-Gal antibodies capable of recognizing both Gal alpha 1----3Gal and B epitopes. It can be concluded from these results that immune tolerance accurately controls the spectrum of natural antibody specificities by preventing the production of antibody clones that can interact with self antigens.
LOGAN: Membership Inference Attacks Against Generative Models (2018)

Abstract Generative models estimate the underlying distribution of a dataset to generate realistic samples according to that distribution. In this paper, we present the first membership inference attacks against generative models: given a data point, the adversary determines whether or not it was used to train the model. Our attacks leverage Generative Adversarial Networks (GANs), which combine a discriminative and a generative model, to detect overfitting and recognize inputs that were part of training datasets, using the discriminator’s capacity to learn statistical differences in distributions. We present attacks based on both white-box and black-box access to the target model, against several state-of-the-art generative models, over datasets of complex representations of faces (LFW), objects (CIFAR-10), and medical images (Diabetic Retinopathy). We also discuss the sensitivity of the attacks to different training parameters, and their robustness against mitigation strategies, finding that defenses are either ineffective or lead to significantly worse performances of the generative models in terms of training stability and/or sample quality.
Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin. (1990)

Ricin A-chain, abrin A-chain, and alpha-trichosanthin are members of a larger group of proteins called ribosome-inactivating proteins. These proteins all function to catalytically inactivate eukaryotic 60 S ribosomal subunits leading to rapid shutdown of protein synthesis. They are homologous in sequence and are probably evolutionarily related. We have determined the complete primary amino acid sequence of alpha-trichosanthin and have found it to be homologous, as expected, to that of abrin A-chain and ricin A-chain. A crystal structure for ricin, which includes ricin A-chain and ricin B-chain, has been determined from x-ray diffraction data. Based on the sequence homologies of these proteins, we fit the primary sequences of abrin A-chain and alpha-trichosanthin to the backbone structure for ricin A-chain and have generated energy-minimized molecular models for them. These models should prove useful in studying the structural-functional relationships of these proteins in particular and of the class in general.
A comparison of melodic database retrieval techniques using sung queries (2002)

Query-by-humming systems search a database of music for good matches to a sung, hummed, or whistled melody. Errors in transcription and variations in pitch and tempo can cause substantial mismatch between queries and targets. Thus, algorithms for measuring melodic similarity in query-by-humming systems should be robust. We compare several variations of search algorithms in an effort to improve search precision. In particular, we describe a new frame-based algorithm that significantly outperforms note-by-note algorithms in tests using sung queries and a database of MIDI-encoded music.
Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. (1982)

Murine embryonal carcinoma cells can differentiate into a varied spectrum of cell types. We observed the abundant and precocious development of neuronlike cells when embryonal carcinoma cells of various pluripotent lines were aggregated and cultured in the presence of nontoxic concentrations of retinoic acid. Neuronlike cells were also formed in retinoic acid-treated cultures of the embryonal carcinoma line, P19, which does not differentiate into neurons in the absence of the drug. The neuronal nature of these cells was confirmed by their staining with antiserum directed against neurofilament protein in indirect immunofluorescence experiments. Retinoic acid-treated cultures also contained elevated acetylcholinesterase activity. Glial cells, identified by immunofluorescence analysis of their intermediate filaments, and a population of fibroblastlike cells were also present in retinoic acid-treated cultures of P19 cells. We did not observe embryonal carcinoma, muscle, or epithelial cells in these cultures. Neurons and glial cells appeared in cultures exposed to retinoic acid for as little as 48 h. We found no evidence for retinoic acid toxicity, suggesting that the effect of the drug was to induce the development of neurons and glia rather than to select against cells differentiating along other developmental pathways.
Immunization of pigs against Streptococcus suis serotype 2 infection using a live avirulent strain. (1997)

Streptococcus suis capsular type 2 is still an important cause of economic losses in the swine industry. At the present time, vaccination of pigs against this infection is generally carried out with autogenous bacterins and results are equivocal. In this study, the protective effect of a live avirulent S. suis type 2 strain (#1330) which had induced a good protection in mice, was evaluated in swine. The experiment was performed in triplicate using 4 week-old piglets. A total of 15 piglets were vaccinated 3 times, 15 others were vaccinated 2 times, and 15 piglets were injected 3 times with sterile Todd-Hewitt broth. Using an indirect ELISA, an increase in the IgG response to S. suis antigens was noted in 27 of the 30 vaccinated piglets. On day 21 post-vaccination, all animals were challenged intravenously with a virulent S. suis type 2 strain (#999). In the 2 vaccinated groups, 26 animals were fully protected. Only 1 out of the 15 piglets vaccinated 3 times developed mild clinical signs. In the group vaccinated twice, 3 piglets showed clinical signs and 1 of them died after the challenge. In the control group, 7 animals died out of the 11 with clinical signs of infection. In conclusion, a protective immunity was observed in swine when using strain 1330. However, more studies are needed to assess the use of a live S. suis strain in a vaccine for pigs.
Upregulation of the α‐secretase ADAM10 – risk or reason for hope? (2010)

A decade ago, a disintegrin and metalloproteinase 10 (ADAM10) was identified as an alpha-secretase and as a key proteinase in the processing of the amyloid precursor protein. Accordingly, the important role that it plays in Alzheimer's disease was manifested. Animal models with an overexpression of ADAM10 revealed a beneficial profile of the metalloproteinase with respect to learning and memory, plaque load and synaptogenesis. Therefore, ADAM10 presents a worthwhile target with respect to the treatment of a neurodegenerative disease such as Morbus Alzheimer. Initially, ADAM10 was suggested to be an enzyme, shaping the extracellular matrix by cleavage of collagen type IV, or to be a tumour necrosis factor alpha convertase. In a relatively short time, a wide variety of additional substrates (with amyloid precursor protein probably being the most prominent) has been identified and the search is still ongoing. Hence, any side effects concerning the therapeutic enhancement of ADAM10 alpha-secretase activity have to be considered. The present review summarizes our knowledge about the structure and function of ADAM10 and highlights the opportunities for enhancing the expression and/or activity of the alpha-secretase as a therapeutic target.
Resistance to Compression Behavior of Alpaca and Wool (2004)

This study compares the resistance to compression behavior of wool and alpaca fibers. It shows that alpaca fibers have a much lower resistance to compression than wool, and there is little correlation between the resistance to compression and the curvature for alpaca fibers. Yet for wool fibers, the correlation between resistance to compression and curvature is very strong and positive. The differences in fiber curvature and scale profiles of alpaca and wool, together with the test method for resistance to compression, may explain their different resistances to compression.