gebhart/patrepeval huggingface dataset:
Novel Cardiac Precursor-Like Cells from Human Menstrual Blood-Derived Mesenchymal Cells (2008)

Stem cell therapy can help repair damaged heart tissue. Yet many of the suitable cells currently identified for human use are difficult to obtain and involve invasive procedures. In our search for novel stem cells with a higher cardiomyogenic potential than those available from bone marrow, we discovered that potent cardiac precursor-like cells can be harvested from human menstrual blood. This represents a new, noninvasive, and potent source of cardiac stem cell therapeutic material. We demonstrate that menstrual blood-derived mesenchymal cells (MMCs) began beating spontaneously after induction, exhibiting cardiomyocyte-specific action potentials. Cardiac troponin-I-positive cardiomyocytes accounted for 27%-32% of the MMCs in vitro. The MMCs proliferated, on average, 28 generations without affecting cardiomyogenic transdifferentiation ability, and expressed mRNA of GATA-4 before cardiomyogenic induction. Hypothesizing that the majority of cardiomyogenic cells in MMCs originated from detached uterine endometrial glands, we established monoclonal endometrial gland-derived mesenchymal cells (EMCs), 76%-97% of which transdifferentiated into cardiac cells in vitro. Both EMCs and MMCs were positive for CD29, CD105 and negative for CD34, CD45. EMCs engrafted onto a recipient's heart using a novel 3-dimensional EMC cell sheet manipulation transdifferentiated into cardiac tissue layer in vivo. Transplanted MMCs also significantly restored impaired cardiac function, decreasing the myocardial infarction (MI) area in the nude rat model, with tissue of MMC-derived cardiomyocytes observed in the MI area in vivo. Thus, MMCs appear to be a potential novel, easily accessible source of material for cardiac stem cell-based therapy.
Online Time-of-Flight Mass Spectrometric Analysis of Peptides Separated by Capillary Electrophoresis (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOnline Time-of-Flight Mass Spectrometric Analysis of Peptides Separated by Capillary ElectrophoresisLiling. Fang, Rong. Zhang, Evan R. Williams, and Richard N. ZareCite this: Anal. Chem. 1994, 66, 21, 3696–3701Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November 1994https://pubs.acs.org/doi/10.1021/ac00093a025https://doi.org/10.1021/ac00093a025research-articleACS PublicationsRequest reuse permissionsArticle Views318Altmetric-Citations118LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Insulin-like growth factors and risk of kidney cancer in men (2010)

Low serum IGF-I levels in this cohort of older middle-aged male smokers are associated with increased kidney cancer risk, independent of IGFBP-3.
Targeting Intracellular Targets (2004)

Many therapeutic agents have intracellular compartments as their site of action. Targeted delivery of these agents to their specific intracellular targets could result in enhanced therapeutic efficacy and reduced toxicity. Various carriers have been shown useful in targeted delivery of different classes of therapeutic agents. Among these carriers, biodegradable nanoparticles formulated from biocompatible polymers poly(D,L-lactide-co-glycolide) (PLGA) and polylactide (PLA) have shown the potential for sustained intracellular delivery of different therapeutic agents. In this review, we discuss different intracellular targets, barriers to intracellular delivery, mechanism and pathways of intracellular delivery, and various carriers and approaches that have been investigated for intracellular drug delivery.
A Protocol for Preferred Access in Packet-Switching Radio Networks (1983)

A protocol that provides multiple access with two levels of priority to a common radio channel is described. A probabilistic model of the protocol is presented and its analysis yields the throughput-delay characteristics of the two classes of packets supported by that protocol, along with their interaction. The model is analyzed by a method similar to the one used in priority queues; this method allows consideration of each class separately, thus reducing the computational effort considerably.
Roles of thrombin and platelet membrane glycoprotein IIb/IIIa in platelet-subendothelial deposition after angioplasty in an ex vivo whole artery model. (1991)

At shear rates seen in nonstenotic coronary arteries, PPACK and 7E3 are more effective than heparin in reducing platelet deposition at the site of balloon injury. The significant inhibition of platelet deposition by PPACK demonstrates the importance of heparin-resistant thrombin in platelet thrombus formation. The 7E3 results suggest that approximately 70% of platelet deposition at the site of balloon injury is GPIIb/IIIa dependent and that the remaining 30% results from non-GPIIb/IIIa-mediated platelet-subendothelial adhesion. Finally, the ex vivo whole artery system is a useful model for studying platelet-vessel wall interactions under physiologically defined parameters.
Randomized Phase III Trial of Amrubicin Versus Topotecan As Second-Line Treatment for Patients With Small-Cell Lung Cancer (2014)

Amrubicin did not improve survival when compared with topotecan in the second-line treatment of patients with SCLC. OS did not differ significantly between treatment groups, although an improvement in OS was noted in patients with refractory disease treated with amrubicin.
Study on the reaction of methylN-Methyl-N-(6-substituted-5-nitropyrimidin-4-yl)glycinates with sodium alkoxides (2006)

Methyl N-methyl-N-(6-substituted-5-nitropyrimidin-4-yl)glycinates (4a-n), obtained from 6-substituted-4-chloro-5-nitropyrimidines and sarcosine methyl ester (methyl 2-(methylamino)acetate), in the reaction with sodium alkoxides underwent transformations to give different products. N-methyl-N-(5-nitropyrimidin-4-yl)glycinates (4a,i,j) bearing amino and arylamino groups in the position 6 of the pyrimidine ring gave corresponding 6-substituted-4-methylamino-5-nitrosopyrimidines (5a,i,j). In the reaction of N-(6-alkylamino-5-nitropyrimidin-4-yl)-N-methylglycinates (4b,f-h) with sodium alkoxides the corresponding 6-alkylamino-4-methylamino-5-nitrosopyrimidines (5b,f-h) and 5-hydroxy-8-methyl-5,8-dihydropteridine-6,7-diones (6b,f-h) were formed. The main products of the reaction of N-(6-dialkylamino-5-nitropyrimidin-4-yl)-N-methylglycinates (4c-e,k,l), after work-up, were the corresponding 6-dialkylamino-9-methylpurin-8-ones (7c-e,k,l) and 8-alkoxy-6-dialkylamino-9-methylpurines (9c,1,10c,l). Methyl N-methyl-N-{[6-(2-methoxy-oxoethyl)thio]-5-nitropyrimidin-4-yl}glycinate (4n) under the same conditions gave methyl 7-methylaminothiazolo[5,4-d]pyrimidine-2-carboxylate (13). Mechanisms of the observed transformations are discussed.
Expression of ICOS on Human Melanoma-Infiltrating CD4+CD25highFoxp3+ T Regulatory Cells: Implications and Impact on Tumor-Mediated Immune Suppression (2008)

ICOShigh Treg can induce diverse immune responses in RC, depending on activation signals and cytokines present. ICOShigh Treg induce Tr1 or Th2 cells depending on the activation state of RC. In a "Tr1" cytokine milieu, ICOShigh Treg transit to Tr1.
Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization. (1986)

By using in situ hybridization methodology, we have directly examined primary lymph node and peripheral blood from patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex for the presence of human T-lymphotropic virus type III (HTLV-III) viral RNA. Mononuclear cell preparations were hybridized with a 35S-labeled HTLV-III-specific RNA probe and exposed to autoradiographic emulsion for 2 days. HTLV-III-infected cells expressing viral RNA were detected in approximately 86% (6/7) of lymph node and 50% (7/14) of peripheral blood samples studied. However, in all patient samples examined, labeled cells were observed at very low frequency (less than 0.01% of total mononuclear cells). The HTLV-III-infected cells exhibited morphological characteristics consistent with that of lymphocytes and expressed viral RNA at relatively low abundance (20-300 copies per cell). These results demonstrate that HTLV-III expression in lymph node and peripheral blood is very low in vivo. Furthermore, the lymph node hyperplasia observed in HTLV-III-associated lymphadenopathy is not directly due to proliferation of HTLV-III-infected lymphocytes.
Progress towards a hepatitis C virus vaccine (2013)

New drugs to treat hepatitis C are expected to be approved over the next few years which promise to cure nearly all patients. However, due to issues of expected drug resistance, suboptimal activity against diverse hepatitis C virus (HCV) genotypes and especially because of their extremely high cost, it is unlikely that these HCV drugs will substantially reduce the world's HCV carrier population of around 170 million in the near future or the estimated global incidence of millions of new HCV infections. For these reasons, there is an urgent need to develop a prophylactic HCV vaccine and also to determine if therapeutic vaccines can aid in the treatment of chronically infected patients. After much early pessimism on the prospects for an effective prophylactic HCV vaccine, our recent knowledge of immune correlates of protection combined with the demonstrated immunogenicity and protective animal efficacies of various HCV vaccine candidates now allows for realistic optimism. This review summarizes the current rationale and status of clinical and experimental HCV vaccine candidates based on the elicitation of cross-neutralizing antibodies and broad cellular immune responses to this highly diverse virus.
Genetic Evidence That Phosphatidylserine Synthase II Catalyzes the Conversion of Phosphatidylethanolamine to Phosphatidylserine in Chinese Hamster Ovary Cells (1998)

Phosphatidylserine (PS) in mammalian cells is synthesized through the exchange of free L-serine with the base moiety of phosphatidylcholine or phosphatidylethanolamine (PE). The serine base exchange in Chinese hamster ovary (CHO) cells is catalyzed by at least two enzymes, PS synthase (PSS) I and II. A PSS I-lacking mutant of CHO-K1 cells, PSA-3, which exhibits approximately 2-fold lower serine base exchange activity than CHO-K1, is defective in the conversion of phosphatidylcholine to PS but has the ability to convert PE to PS. The PSA-3 mutant requires exogenous PS or PE for cell growth. In the present study, from PSA-3 mutant cells, we isolated a mutant, named PSB-2, with a further decrease in the serine base exchange activity. The activity in the homogenate of PSB-2 mutant cells was approximately 10% that of PSA-3 mutant cells and approximately 5% that of CHO-K1 cells. The PSB-2 mutant exhibited an approximately 80% reduction in the PSS II mRNA level relative to that in PSA-3 mutant and CHO-K1 cells. These results showed that the PSB-2 mutant is defective in PSS II. Like the PSA-3 mutant, the PSB-2 mutant grew well in medium supplemented with PS. However, in the medium supplemented with PE, the PSB-2 mutant was incapable of growth, in contrast to the PSA-3 mutant. In the medium with exogenous PE, the PSB-2 mutant was defective in PS biosynthesis, whereas the PSA-3 mutant synthesized a normal amount of PS. A metabolic labeling experiment with exogenous [32P]PE revealed that the PSB-2 mutant was defective in the conversion of exogenous PE to PS. This defect and the growth and PS biosynthetic defects of the PSB-2 mutant cultivated with exogenous PE were complemented by the PSS II cDNA. In addition, the cDNA of the other PS synthase, PSS I, was shown not to complement the defect in the conversion of exogenous PE to PS of the PSB-2 mutant, implying that PSS I negligibly contributes to the conversion of PE to PS in CHO-K1 cells. These results indicated that PSS II is critical for the growth and PS biosynthesis of PSA-3 mutant cells cultivated with exogenous PE and suggested that most of the PS formation from PE in CHO-K1 cells is catalyzed by PSS II.
Power-aware data management for small devices (2002)

Pervasive computing devices such as Personal Digital Assistants (PDAs) and laptop computers are becoming increasingly ubiquitous. The future promises even more advanced devices such as digital watches, jewelry, and even clothing. However, as pervasive devices become more widely used for more advanced applications, their resource limitations are becoming more apparent. In this work, we focus on data management and power limitations. We investigate the benefit of using power-aware schemes to automatically manage content across a collection of devices and prolong data availability. We monitor the available energy supply on each device and migrate content from devices that are in danger of dying. In our simulated environment, we have found that, using intelligent techniques for data management can increase the amount of time a collection of devices remains usable by over 2 times. Furthermore, our techniques can perform autonomously, independent of user intervention.
On a scalable topology for lightwave networks (2002)

Most of the logical topologies for lightwave networks available in the literature are not scalable. As a result, for these topologies, the addition of new stations to an existing network is extremely difficult. In this paper, we present a scalable topology for lightwave networks and discuss how the expansion of the network can be handled. We analyze the properties of the proposed network and compare it with other network interconnections. Assuming a multistar implementation, we also address the channel assignment problem.
Curing Affluenza? A Critique of Recent Changes to the Taxation of Capital Gains in Australia (2000)

Australia, in 1985, was one of the last of the Organization for Economic Development and Co-operation countries to introduce a Capital Gains Tax (CGT) regime. It is argued that many of these recent changes to the CGT regime is not doing anything for the integrity of the Australian tax system because they flaunt many of the essential principles upon which good tax design should be based, and are unlikely to satisfy the criterion of revenue neutrality that was supposed to be a key feature of the Ralph Review reforms.
Mini-Gut Organoids: Reconstitution of the Stem Cell Niche (2015)

In the adult mammalian body, self-renewal of tissue stem cells is regulated by extracellular niche environments in response to the demands of tissue organization. Intestinal stem cells expressing Lgr5 constantly self-renew in their specific niche at the crypt bottom to maintain rapid turnover of the epithelium. Niche-regulated stem cell self-renewal is perturbed in several mouse genetic models and during human tumorigenesis, suggesting roles for EGF, Wnt, BMP/TGF-β, and Notch signaling. In vitro niche reconstitution capitalizing on this knowledge has enabled the growth of single intestinal stem cells into mini-gut epithelial organoids comprising Lgr5(+) stem cells and all types of differentiated lineages. The mini-gut organoid culture platform is applicable to various types of digestive tissue epithelium from multiple species. The mechanism of self-renewal in organoids provides novel insights for organogenesis, regenerative medicine, and tumorigenesis of the digestive system.
Sequence analysis and editing for bisulphite genomic sequencing projects (2007)

Bisulphite genomic sequencing is a widely used technique for detailed analysis of the methylation status of a region of DNA. It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region. Since this two-step procedure replaces all unmethylated cytosine bases with thymine, PCR products derived from unmethylated templates contain only three types of nucleotide, in unequal proportions. This can create a number of technical difficulties (e.g. for some base-calling methods) and impedes manual analysis of sequencing results (since the long runs of T or A residues are difficult to align visually with the parent sequence). To facilitate the detailed analysis of bisulphite PCR products (particularly using multiple cloned templates), we have developed a visually intuitive program that identifies the methylation status of CpG dinucleotides by analysis of raw sequence data files produced by MegaBace or ABI sequencers as well as Staden SCF trace files and plain text files. The program then also collates and presents data derived from independent templates (e.g. separate clones). This results in a considerable reduction in the time required for completion of a detailed genomic methylation project.
Detailed quality of life assessment in patients treated with primary radiotherapy for squamous cell cancer of the base of the tongue (1997)

The overwhelming majority of patients achieved excellent functional status and quality of life and could maintain their prediagnosis earning potential and employment status after primary radiation for advanced base of tongue cancer.
Regulation of sex hormone dependent growth by a serum borne inhibitor (2000)

This thesis project focused on understanding the basic process controlling cell proliferation in sex-steroid hormone dependent cancers. The availability of inculture models using cloned cell lines offers the greatest advantage for studying the control of this event. Incubation of cloned sex-hormone sensitive cells in medium containing increasing concentrations of sex-hormone stripped serum, results in a dose dependent growth inhibition; this inhibition is reversed by the addition of physiological concentrations of steroid hormones. The mechanisms explaining this phenomenon are not yet fully understood, but different theories propose the existence in serum of a sex hormone binding protein with growth inhibitory properties. We were able to identify a protein that specifically binds sex hormones in rat and horse serum with affinities 10-fold lower to the ones observed with the classic sex-hormone binding globulin (SHBG) in humans. Purification of this protein on a large scale Lowed a more detailed analysis. The putative sex-hormone binding protein has an apparent molecular weight of 386 KDa. SDS-PAGE with commassie staining of the purified product, displayed a pattern non-characteristic of SMG, but all bands cross-reacted with a commercial anti-SMG antibody in western analysis. Titrations of the purified product on cell proliferation assays using sex-hormone dependent lines, resulted in a dose dependent growth inhibition. This inhibition was reversed by the addition of sex hormones. Our results indicate that we have identified and purified a sex-hormone binding protein in serum with characteristics similar to SHBG and with cell growth inhibitory properties.
'BLUE LAWS' STILL APPLY (1999)

SUBTITLE: DELIVERIES OF WINE, BEER AND SPIRITS REQUIRE DIFFERENT HANDLING ACCORDING TO STATE, LOCAL LAWS.
New Condensed Tri- and Tetracyclic Pyrazole Ring System (1984)

Researoh I n s t i t u t e , Pal6rikeva 31, 81 1 04 Bratislava, C l e o b s l e ~a ~b s t r a o t -1 -Phenylpyraeelo [4,3-e] -lo C1, z-d -5ino and it.5-am anelowe were synthesized from ethyl 5-amlsa-1-pwlmeole-4-aarbor- ylate.Additieaal a s o h rims cumelatioru on t h e p).ra!dine Part of the
A comparison of adaptive algorithms based on the methods of steepest descent and random search (1976)

This paper compares the performance characteristics of three algorithms useful in adjusting the parameters of adaptive systems: the differential (DSD) and least-mean-square (LMS) algorithms, both based on the method of steepest descent, and the linear random search (LRS) algorithm, based on a random search procedure derived from the Darwinian concept of "natural selection." The LRS algorithm is presented here for the first time. Analytical expressions are developed that define the relationship between rate of adaptation and "misadjustment," a dimensionless measure of the difference between actual and optimal performance due to noise in the adaptive process. For a fixed rate of adaptation it is shown that the LMS algorithm, which is the most efficient, has a misadjustment proportional to the number of adaptive parameters, while the DSD and LRS algorithms have misadjustments proportional to the square of the number of adaptive parameters. The expressions developed are verified by computer simulations that demonstrate the application of the three algorithms to system modeling problems, of the LMS algorithm to the cancelling of broadband interference in the sidelobes of a receiving antenna array, and of the DSD and LRS algorithms to the phase control of a transmitting antenna array. The second application introduces a new method of constrained adaptive beamforming whose performance is not significantly affected by element nonuniformity. The third application represents a class of problems to which the LMS algorithm in the basic form described in this paper is not applicable.
Übersicht (1963)

Die gesteigerten Anforderungen an die Industrieprodukte und die weitläufigen Forschungen auf allen Gebieten der Technik erfordern immer mehr die Durchführung von verfeinerten, umfangreichen Berechnungen. Diese numerischen Berechnungen lassen sich mit den herkömmlichen Hilfsmitteln wie Rechenschieber, Zahlentafeln, Tischrechenmaschinen usw. nicht mehr innert nützlicher Frist ausführen. Es wurden daher Rechenautomaten gebaut, welche die monotone Rechenarbeit dem Techniker und Ingenieur abnehmen können. Verschiedene Grossfirmen haben in letzter Zeit in ihren Betrieben sogenannte Rechenzentren errichtet, in denen Rechenautomaten aufgestellt sind, womit die anfallenden Rechenprobleme gelöst werden. Daneben betreiben schon seit einiger Zeit die Hersteller von Rechenautomaten und andere Interessenten eigene Rechenzentren, die durch die Industrie mietweise benützt werden können. Neben der Maschinen- und Elektroindustrie und den Chemiebetrieben verwenden auch Bauingenieurbüros Rechenautomaten zur Lösung von umfangreichen Problemen aus der Baustatik und der Vermessung. Schliesslich sollen auch noch die Hochschulen erwähnt werden, welche ebenfalls Rechenautomaten besitzen, die sie zum Teil sogar selber entworfen und gebaut haben, um damit mathematische Forschung betreiben zu können und auch um die Methoden des programmgesteuerten Rechnens weiter zu entwickeln.