gebhart/patrepeval huggingface dataset:
Bcl-2 overexpression is associated with resistance to paclitaxel, but not gemcitabine, in multiple myeloma cells. (1998)

Multiple myeloma (MM) is an incurable disease despite an initial response-rate of >60% with conventional or high-dose chemotherapy using glucocorticosteroids (i.e. dexamethasone), or alkylating agents (i.e. melphalan). Although these agents are capable of inducing complete remission (CR) in >50% of MM patients, resistance develops rapidly, in >90% of patients, within 2 years of treatment. Therefore, there is a need for new drugs for the treatment of relapsing and refractory MM patients. Gemcitabine (GEM) is a pyrimidine analog that blocks DNA synthesis, whereas, paclitaxel (TAX) is a mitotic spindle poison that promotes microtubular aggregation. Since it appears that these two drugs have different cellular targets, we examined the effect of each drug individually for several parameters and for possible synergy. We studied the cytotoxic effect of TAX and GEM on MM cells expressing varying levels of the antiapoptotic protein bcl-2, which is overexpressed in the majority of myeloma cell from MM patients. We found that both drugs are cytotoxic by inducing apoptosis, however, the extent of apoptosis with TAX, but not with GEM was dependent on the levels of bcl-2 expression. We further investigated the effect of TAX and GEM on the cell cycle distribution and on the levels of bcl-2. The results indicate that the two drugs have different modes of action with respect to each parameter tested. TAX induced arrest of the cells in the G2/M phase of the cell cycle, regardless of bcl-2 levels, however, apoptosis was induced in mitotic cells expressing relatively low levels of bcl-2. In contrast, GEM caused apoptosis of cells in the S-phase, regardless of level of bcl-2 expression. A major difference between TAX and GEM was in their effects on the levels of bcl-2. Whereas, TAX induced an early downregulation of bcl-2 (only in the cells with relatively low levels of bcl-2), treatment with GEM did not affect bcl-2 levels. The effects of TAX on both the cell cycle and bcl-2 were detected very early (4-8 h) and preceded the onset of apoptosis. GEM and TAX act synergistically, at low doses (IC50 of 0.5 microM for GEM and 0.025 microM for TAX), to effectively kill bcl-2 overexpressing cells that are resistant to higher doses (0.25 microM) of TAX alone. Therefore, we have initiated a phase II clinical trial of TAX and GEM for MM patients refractory to current therapy.
Video rewrite: visual speech synthesis from video. (1997)

Video Rewrite uses existing footage to create automatically new video of a person mouthing words that she did not speak in the original footage. Video Rewrite uses computer-vision techniques to track points on the speaker&amp;apos;s mouth in the training footage, and morphing techniques to combine these mouth gestures into the final video sequence. The new video combines the dynamics of the original actor&amp;apos;s articulations with the mannerisms and setting dictated by the background footage. Video Rewrite is the first facial-animation system to automate all the labeling and assembly tasks required to resync existing footage to a new soundtrack. 1 1 INTRODUCTION Humans are extremely sensitive to the synchronization between speech and lip motions. For example, the special effects in Forrest Gump are compelling because the Kennedy and Nixon footage is lip synched to the movie&amp;apos;s new soundtrack. In contrast, close ups in dubbed movies are often disturbing due to the lack of lip sync. Our system, Vid...
Protective CD4+and CD8+T Cells against Influenza Virus Induced by Vaccination with Nucleoprotein DNA (1998)

DNA vaccination is an effective means of eliciting both humoral and cellular immunity, including cytotoxic T lymphocytes (CTL). Using an influenza virus model, we previously demonstrated that injection of DNA encoding influenza virus nucleoprotein (NP) induced major histocompatibility complex class I-restricted CTL and cross-strain protection from lethal virus challenge in mice (J. B. Ulmer et al., Science 259:1745-1749, 1993). In the present study, we have characterized in more detail the cellular immune responses induced by NP DNA, which included robust lymphoproliferation and Th1-type cytokine secretion (high levels of gamma interferon and interleukin-2 [IL-2], with little IL-4 or IL-10) in response to antigen-specific restimulation of splenocytes in vitro. These responses were mediated by CD4+ T cells, as shown by in vitro depletion of T-cell subsets. Taken together, these results indicate that immunization with NP DNA primes both cytolytic CD8+ T cells and cytokine-secreting CD4+ T cells. Further, we demonstrate by adoptive transfer and in vivo depletion of T-cell subsets that both of these types of T cells act as effectors in protective immunity against influenza virus challenge conferred by NP DNA.
Prospective Study of Determinants and Outcomes of Deferred Treatment or Watchful Waiting Among Men With Prostate Cancer in a Nationwide Cohort (2009)

In this nationwide cohort, more than half the men who opted for DT remained without treatment for 7.7 years after diagnosis. Older men and men with lesser cancer severity at diagnosis were more likely to remain untreated. PCa mortality did not differ between DT and active treatment patients.
Exome Sequencing Reveals Comprehensive Genomic Alterations across Eight Cancer Cell Lines (2011)

It is well established that genomic alterations play an essential role in oncogenesis, disease progression, and response of tumors to therapeutic intervention. The advances of next-generation sequencing technologies (NGS) provide unprecedented capabilities to scan genomes for changes such as mutations, deletions, and alterations of chromosomal copy number. However, the cost of full-genome sequencing still prevents the routine application of NGS in many areas. Capturing and sequencing the coding exons of genes (the "exome") can be a cost-effective approach for identifying changes that result in alteration of protein sequences. We applied an exome-sequencing technology (Roche Nimblegen capture paired with 454 sequencing) to identify sequence variation and mutations in eight commonly used cancer cell lines from a variety of tissue origins (A2780, A549, Colo205, GTL16, NCI-H661, MDA-MB468, PC3, and RD). We showed that this technology can accurately identify sequence variation, providing ∼95% concordance with Affymetrix SNP Array 6.0 performed on the same cell lines. Furthermore, we detected 19 of the 21 mutations reported in Sanger COSMIC database for these cell lines. We identified an average of 2,779 potential novel sequence variations/mutations per cell line, of which 1,904 were non-synonymous. Many non-synonymous changes were identified in kinases and known cancer-related genes. In addition we confirmed that the read-depth of exome sequence data can be used to estimate high-level gene amplifications and identify homologous deletions. In summary, we demonstrate that exome sequencing can be a reliable and cost-effective way for identifying alterations in cancer genomes, and we have generated a comprehensive catalogue of genomic alterations in coding regions of eight cancer cell lines. These findings could provide important insights into cancer pathways and mechanisms of resistance to anti-cancer therapies.
Towards model-based and ccm-based applications for real-time systems (2004)

New advances in software component and modeling standards promise to ease the burden of development complexity while improving flexibility and reliability. These solutions raise the level of abstraction from object-oriented classes to coarser components and support the creation and configuration of systems using modeling or other specification standards. This paper outlines the challenges inherent in the original Bold Stroke component model developed in the late 1990's and then discusses an evolved component model that leverages work being done in the Object Management Group (OMG) and in government funded research that addresses these issues in a distributed real-time embedded (DRE) application context.
Scalable Routes to Gold Nanoshells with Tunable Sizes and Response to Near‐Infrared Pulsed‐Laser Irradiation (2008)

A simplified synthesis of hollow gold nanoshells 20-50 nm in diameter via the well-established templated galvanic replacement reaction of silver for gold is presented. The surface plasmon resonance absorbance of the nanoshells is tuned using basic colloid chemistry to control the size of the silver templates. The gold nanoshells have an aqueous core and are varied in size and shell thickness depending on the silver/gold reagent ratios. The template replacement chemistry is rapid, highly scalable, uses minimal amounts of toxic reagents, and in many cases is a true one-pot synthesis. The smallest nanoshells (20-nm diameter, 7-nm wall thickness) reach the highest temperature on irradiation with femtosecond light pulses in the near infrared and anneal to form spherical nanoparticles fastest, even though their plasmon resonance does not overlap as well as the larger nanoshells (50-nm diameter, 7-nm wall thickness) with 800-nm wavelength excitation.
A Facile and Convenient Synthesis of 5,5-Dialkylthio- and 5-Alkyl(or Aryl)thio-2,4-pentadienoic Acids (1991)

The hydrolysis of 6,6-dialkylthio- and 6-alkyl(or aryl)thio-1,1,1-trifluoro-3,5-hexadien-2-ones 2, 5 with potassium hydroxide in ethanol proceeds quite easily to give the corresponding 2,4-pentadienoic acids 3, 6, respectively, in excellent yields. Bistrifluoroacetyl compounds 8,10a, b give corresponding monocarboxylic acids 6a, 3a, b in high yields, with accompanied monodecarboxylation.
Double-blind, randomized trial of bupropion SR for the treatment of neuropathic pain (2001)

This placebo-controlled crossover trial showed that bupropion SR (150-300 mg daily) was effective and well tolerated for the treatment of neuropathic pain.
Positioning the Subscriber Loop Network for Digital Services (1982)

The need to enhance the digital transmission capability of the local loop network to provide for new services is widely recognized in the telecommunications industry. Companies around the world [1]-[3] have been working towards this goal and plan to use digital loop carrier (DLC) to meet this demand. Although this approach is generally accepted, there has been little information presented on how to plan for the evolution of the local plant. To address these pressing issues, the Bell System is introducing a concept called fundamental subscriber carrier planning (FSCP). This paper discusses the impact that digital services will have on the subscriber loop network, including loop plant design requirements necessary to position the network for up to 64 kbit/s digital services. Existing cable plant has the capability to support most digital services, but there are specific design requirements that are quite different from those required for the "typical" analog voiceband service. This paper reviews the need to position the loop for digital services, and substantiates the major conclusion that digital loop carrier technology offers a significant advantage when provisioning for digital services.
The durability of parylene coatings on neodymium-iron-boron magnets (1999)

A parylene coating is frequently used to prevent corrosion of neodymium-iron-boron magnets when they are used intra-orally. This in vitro study was designed to test the durability of parylene coating in a simulated oral environment. Single and double parylene-coated magnets were subjected to grinding and crushing forces in an industrial ball mill. The results demonstrate that abrasion and wear was visible around the edges after 1 hour of testing, with a breach of the coating noted under high magnification scanning electron microscopy (SEM). The conclusion of the study is that parylene coating is unlikely to withstand intra-oral forces. The shape of the magnets, the manufacturing process involved in their production, and the thickness of the parylene coating are important factors to consider with respect to the durability of magnets used in the mouth.
Molded continuous poly(styrene-co-divinylbenzene) rod as a separation medium for the very fast separation of polymers Comparison of the chromatographic properties of the monolithic rod with columns packed with porous and non-porous beads in high-performance liquid chromatography of polystyrenes (1996)

Gradient elution separations of polystyrene standards in a monolithic molded 50×8 mm I.D. poly(styrene-co-divinylbenzene) rod column and in 50×8 mm I.D. and 30×4.1 mm I.D. columns packed with porous and non-porous poly(styrene-co-divinylbenzene) beads has been carried out. All of these separation media differ in shape and porosity. Excellent separations of eight polystyrene standards were achieved with both the molded monolithic rod and porous beads at moderate flow-rates. However, the monolithic medium proved to be superior for high-speed separations using very steep gradients at a flow-rate of 20 ml/min. Three polystyrene standards were separated in the rod column within 4 s. The separation in the column packed with non-porous beads was poor at flow-rates of 2–8 ml/min, while higher flow-rates led to an unacceptably high back pressure.
History of the Quality Debate (2004)

The term ‘quality’ as used in recent education discourse has its origins in the factory floor and production, as a measure of control of the features of merchandise produced. In the form of measureable specifications, it is used by national bureaucracies and international aid and lending agencies as lending itself to scientific management of and monitoring investments in education. Going back to the concept of ‘quality’ as the essential character of a thing, the paper argues that quality has always been integral to the concept of education, deriving from what is considered worthwhile as an aim or experience in education. The paper traces the history of the term beginning with important changes in the concept of education influenced by education thinkers and moving to the role of the concept in the context of universalisation of education, the growing importance of democracy as a political choice, and economic globalisation. The final section of the paper draws out implications for teacher professionalism, influences onpolicy, the idea of accountability and the place of philosophical perspective and cultural sensitivity in education.
The Efficient Cellular Uptake of High Density Lipoprotein Lipids via Scavenger Receptor Class B Type I Requires Not Only Receptor-mediated Surface Binding but Also Receptor-specific Lipid Transfer Mediated by Its Extracellular Domain (1998)

The class B type I scavenger receptor, (SR-BI), is a member of the CD36 superfamily of proteins and is a physiologically relevant, high affinity cell surface high density lipoprotein (HDL) receptor that mediates selective lipid uptake. The mechanism of selective lipid uptake is fundamentally different from that of classic receptor-mediated uptake via coated pits and vesicles (e.g. the low density lipoprotein receptor pathway) in that it involves efficient transfer of the lipids, but not the outer shell proteins, from HDL to cells. The abilities of SR-BI and CD36, both of which are class B scavenger receptors, to bind HDL and mediate cellular uptake of HDL-associated lipid when transiently expressed in COS cells were examined. For these experiments, the binding of HDL to cells was assessed using either 125I- or Alexa (a fluorescent dye)-HDL in which the apolipoproteins on the surface of the HDL particles were covalently modified. Lipid transfer was measured using HDL noncovalently labeled by the fluorescent lipid 1,1'-dioctadecyl-3,3, 3',3'-tetramethylindocarbocyanine perchlorate. Although both mSR-BI and human CD36 (hCD36) could mediate the binding of HDL in a punctate pattern across the surfaces of cells, only mSR-BI efficiently mediated the transfer of lipid to the cells. Analysis of point mutants established that the major sites of fatty acylation of mSR-BI are Cys462 and Cys470 and that fatty acylation is not required for receptor clustering, HDL binding, or efficient lipid transfer. Generation of mSR-BI/hCD36 domain swap chimeras showed that the differences in lipid uptake activities between mSR-BI and hCD36 were not due to differences between their two sets of transmembrane and cytoplasmic domains but rather result from differences in their large extracellular loop domains. These results show that high affinity binding to a cell surface receptor is not sufficient to ensure efficient cellular lipid uptake from HDL. Thus, SR-BI-mediated binding combined with SR-BI-dependent facilitated transfer of lipid from the HDL particle to the cell appears to be the most likely mechanism for the bulk of the selective uptake of cholesteryl esters from HDL to the liver and steroidogenic tissues.
Forming an α-Hemolysin Nanopore for Single-Molecule Analysis (2009)

Nanopore analysis of single molecules can be performed by measuring the modulation in ionic current passing through the nanopore while an individual biomolecule such as DNA or RNA is resident in, translocating through, or otherwise interacting with the pore. The corresponding current signature has been shown to reveal properties of the biomolecule and information on its interactions with the pore. The alpha-hemolysin nanopore remains the pore of choice, particularly for single-molecule analysis of nucleic acids, because of its internal dimensions, hydrophilicity, and low-noise characteristics. In this chapter we present a detailed protocol for forming a robust alpha-hemolysin nanopore (or multiple nanopores) for single-molecule analysis.
Catalytic activity of elastase in reverse micelles. (1993)

The activity of porcine pancreatic elastase has been studied in reverse micelles formed by AOT (sodium bis(2-ethylhexyl) sulfosuccinate) in isooctane. For the two substrates succinyl-L-Ala-L-Ala-L-Ala-p-nitroanilide and succinyl-L-Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide, the catalytic constant, kcat, in reverse micelles increases with increasing wo until, at high wo, the value of kcat measured in bulk buffer solution is approached (wo = [H2O/]AOT]). In analogy to alpha-chymotrypsin--and in apparent contrast to many other enzymes--elastase does not show a maximum in the kcat-wo profile. Within the wo range of 8 to 35, for both substrates, the Michaelis constant Km (as expressed relative to the total volume of the solution, Km,overall) increases with increasing wo.
A First Implementation of an Automated Reel‐to‐Reel Fluidic Self‐Assembly Machine (2014)

A first automated reel-to-reel fluidic selfassembly process for macroelectronic applications is reported. This system enables high-speed assembly of semiconductor dies (15 000 chips per hour using a 2.5 cm-wide web) over large-area substrates. The optimization of the system (>99% assembly yield) is based on identification, calculation, and optimization of the relevant forces. As an application, the production of a solid-state lighting panel is discussed, involving a novel approach to apply a conductive layer through lamination.
[Down-regulated expression of UNC5b related to hepatocellular carcinoma angiogenesis]. (2009)

Down-regulation of UNC5b gene expression is related to angiogenesis of HCC, which may be associated with the progression of HCC.
The Effects of Alkoxy Functional Groups on Atmospheric‐Pressure Chemical Vapor Deposition Using Alkoxysilane and Ozone (1995)

The effects of alkoxy functional groups on atmospheric‐pressure chemical vapor deposition (APCVD) using alkoxysilane and ozone are investigated from the standpoint of the molecular structure of the alkoxysilane molecules. Deposition rate and step coverage are evaluated for three kinds of alkoxysilane sources , (called TEOS), and . The addition of three kinds of alcohols, , , and , to the system is also investigated. Both the deposition rates and step coverage are associated with the RO‐ functional groups of the alkoxysilane molecule. , which contains the smallest alkoxy (RO‐) groups, has the highest deposition rate and the best flow characteristics among the three silicon sources. With added alcohol: (i) the OH‐ group scavenges ozone molecules and affects the deposition rate, and (ii) the RO‐ group affects only the step coverage. The flow property is also affected by adding RO‐ fragments in reaction system.
Schizophrenia: Etiology and Course (2004)

Decades of research on schizophrenia have not produced major breakthroughs, but gradual progress has been made in identifying risk factors and clarifying the nature of the etiologic process. This article provides an overview of trends in research findings as well as current assumptions about the interplay between environmental and genetic factors in the etiology of schizophrenia. Based on the cumulative findings, it appears that both genetic and prenatal factors can give rise to constitutional vulnerability. Subsequent neuromaturational processes, especially those that occur during adolescence, and exposure to stressful events can trigger the behavioral expression of this vulnerability.
Effect of Single-Drug Therapy on Reduction of Left Atrial Size in Mild to Moderate Hypertension (1998)

Antihypertensive drugs differ in their effects on left atrial size. Hydrochlorothiazide was associated with greater overall reduction of left atrial size than other drugs effective for the treatment of hypertension. Reduction of left atrial size with therapy is in part independent of factors known to influence left atrial size, including LV mass and reduction of LV mass with treatment. The clinical benefit of reducing left atrial size with antihypertensive treatment remains to be determined.
Coupled-stripe in-phase operation of planar native-oxide index-guided AlyGa1−yAs-GaAs-InxGa1−xAs quantum-well heterostructure laser arrays (1992)

High-performance, coupled-stripe, planar, index-guided AlyGa1−yAs-GaAs-InxGa1−xAs quantum-well heterostructure (QWH) laser arrays are fabricated by the formation of a relatively thick, current-blocking, native oxide from the high-gap AlyGa1−yAs upper confining layer between active stripes. Precise control of the thickness of the native oxide layer between emitters provides a means of varying the index step between stripes, and permits tailoring of the optical profile to produce in-phase operation. The 10-stripe coupled QWH laser arrays (∼3-μm-wide stripes on 4 μm centers) exhibit near-diffraction-limited, single-lobed, far-field patterns with low continuous (cw) thresholds (∼45 mA) and cw output powers (total external differential quantum efficiency ≳50%) of over 100 mW per uncoated facet.
Mitochondrial Targeting Signal of Rat Liver Monoamine Oxidase B Is Located at Its Carboxy Terminus (1992)

Monoamine oxidase B, a typical intrinsic protein of the outer mitochondrial membrane, has an uncleavable targeting signal and is inserted into the membrane without proteolytic maturation. To investigate the region responsible for targeting the enzyme to the outer mitochondrial membrane, various mutated proteins were expressed in cultured mammalian cells, and the distributions of the expressed proteins were analyzed by immunofluorescence microscopy and subcellular fractionation. Deletion of the carboxy-terminal 28 amino acids of monoamine oxidase B abolished the transfer of the enzyme to mitochondria, while the deletion of the amino-terminal 55 amino acids had no effect on the transfer to mitochondria. The existence of the targeting signal at the carboxy-terminal portion of the enzyme was confirmed by using hybrid proteins in which the amino- or carboxy-terminal portion of the enzyme was fused to the hydrophilic portion of cytochrome b5. The fused protein with the carboxy-terminal 29 amino acid residues of monoamine oxidase B was localized in mitochondria, whereas that with 10 amino acids remained in the cytoplasm. These results indicate that the targeting signal of monoamine oxidase B is present within its carboxy-terminal 29 amino acid residues.