gebhart/patrepeval huggingface dataset:
An Injectable Hybrid Hydrogel with Oriented Short Fibers Induces Unidirectional Growth of Functional Nerve Cells (2017)

To regenerate soft aligned tissues in living organisms, low invasive biomaterials are required to create 3D microenvironments with a structural complexity to mimic the tissue's native architecture. Here, a tunable injectable hydrogel is reported, which allows precise engineering of the construct's anisotropy in situ. This material is defined as an Anisogel, representing a new type of tissue regenerative therapy. The Anisogel comprises a soft hydrogel, surrounding magneto-responsive, cell adhesive, short fibers, which orient in situ in the direction of a low external magnetic field, before complete gelation of the matrix. The magnetic field can be removed after gelation of the biocompatible gel precursor, which fixes the aligned fibers and preserves the anisotropic structure of the Anisogel. Fibroblasts and nerve cells grow and extend unidirectionally within the Anisogels, in comparison to hydrogels without fibers or with randomly oriented fibers. The neurons inside the Anisogel show spontaneous electrical activity with calcium signals propagating along the anisotropy axis of the material. The reported system is simple and elegant and the short magneto-responsive fibers can be produced with an effective high-throughput method, ideal for a minimal invasive route for aligned tissue therapy.
In Minor Dermatological and Needle Puncture Procedures (2010)

The lidocaine/tetracaine medicated plaster comprises a lidocaine/tetracaine 70 mg/70 mg patch and a controlled heat-assisted drug delivery pod that increases the diffusion of lidocaine and tetracaine into the dermis. Following a 1-hour application period, systemic absorption of lidocaine or tetracaine from the plaster was minimal. The lidocaine/tetracaine medicated plaster provided effective pain relief for adult (including elderly) patients undergoing minor dermatological procedures and for adult and paediatric patients undergoing vascular access procedures. In randomized, double-blind clinical trials, patientreported median pain scores were significantly lower with the lidocaine/tetracaine medicated plaster than with an identical plaster containing placebo in patients undergoing minor dermatological or vascular access procedures. Furthermore, patient-reported median pain scores were significantly lower with the lidocaine/tetracaine medicated plaster than with a lidocaine/prilocaine cream in patients undergoing vascular access procedures. In a large, randomized, double-blind trial in paediatric patients undergoing venipuncture, the overall incidence of pain was significantly lower with the lidocaine/tetracaine medicated plaster than with a lidocaine/prilocaine plaster. The lidocaine/tetracaine medicated plaster was well tolerated, with the most frequent treatmentrelated adverse events resolving spontaneously. Features and properties of lidocaine/ tetracaine medicated plaster (Rapydan®)
Stability of the Heme-Globin Linkage in αβ Dimers and Isolated Chains of Human Hemoglobin (1996)

The stability of the heme-globin linkage in alphabeta dimers and in the isolated chains of human hemoglobin has been probed by studying the transfer of heme from the proteins immobilized onto CNBr-activated Sepharose 4B to human albumin. The kinetic and equilibrium features of the reaction have been measured spectrophotometrically given the stability of the heme donors and the ease with which heme donor and acceptor can be separated. Isolated alpha and beta chains transfer heme to albumin at similar rates (1 6 x 10(-2) s-1 at pH 9.0 and 20 degrees C) in the ferrous CO-bound and in the ferric state. In alpha beta dimers the heme-globin linkage is strengthened considerably, albeit to a different extent in the ferrous CO-bound and ferric met-aquo derivatives. Only in the latter heme is lost at a measurable rate, 0.065 +/- 0.011 x 10(-2) s-1 for alpha heme and 2.8 +/- 0.6 x 10(-2) s-1 for beta heme at pH 9.0 and 20 degrees C, which is very close to the rate measured with soluble met-aquo-hemoglobin at micromolar concentrations. These results indicate that in human hemoglobin the heme-globin linkage in the alpha chains is stabilized by interactions between unlike chains at the alpha1 beta1 interface, whereas heme binding to the beta chains is stabilized by interactions at the alpha1beta2 interface. These long range factors have to be taken into account in addition to the local factors at the heme pocket when evaluating the effect of point mutation and chemical modification.
Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor (1991)

We tested the ability of recombinant human stem cell factor (SCF) to stimulate isolated marrow precursor cells to form colonies in semisolid media and to generate colony-forming cells (CFC) in liquid culture. SCF, in combination with interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or granulocyte colony-stimulating factor (G-CSF) caused CD34+ cells to form increased numbers of granulocyte-macrophage colonies (CFU-GM), and to form macroscopic erythroid burst-forming units (BFU-E) in the presence of IL-3, erythropoietin (Epo), and SCF. We tested isolated CD34+lin- cells, a minor subset of CD34+ cells that did not display antigens associated with lymphoid or myeloid lineages, and CD34+lin+ cells, which contain the vast majority of CFC, and found that the enhanced colony growth was most dramatic within the CD34+lin- population. CD34+lin- cells cultured in liquid medium containing SCF combined with IL-3, GM-CSF, or G-CSF gave rise to increased numbers of CFC. Maximal numbers of CFU-GM were generated from CD34+lin- cells after 7 to 21 days of culture, and required the presence of SCF from the initiation of liquid culture. The addition of SCF to IL-3 and/or G-CSF in cultures of single CD34+lin- cells resulted in increased numbers of CFC due to the proliferation of otherwise quiescent precursors and an increase in the numbers of CFC generated from individual precursors. These studies demonstrate the potent synergistic interaction between SCF and other hematopoietic growth factors on a highly immature population of CD34+lin- precursor cells.
Design and Construction of a New Haptic Interface (2000)

Abstract Virtual Reality becomes more and more important within the product development process. It enables the engineer to realize constraints or mistakes in the product design at a very early stage by viewing the digital geometric prototype. Beside viewing the design of a product, additional functionalities like simulation of assembling, the physically correct behavior of a machine or the machine control come into focus of interest. Therefore, the interaction modality of haptic feedback gains more and more importance for simulation tasks in virtual environments. However there are only a few portable haptic interfaces with which the user can experience in a natural way the sensation of force feedback. The scope of this paper is to present a new passive haptic interface that is lightweight and easy to use. Furthermore it has no constraints in the workspace and applies high forces to the fingertips of the user by blocking the natural grasping.
The B73 Maize Genome: Complexity, Diversity, and Dynamics (2009)

We report an improved draft nucleotide sequence of the 2.3-gigabase genome of maize, an important crop plant and model for biological research. Over 32,000 genes were predicted, of which 99.8% were placed on reference chromosomes. Nearly 85% of the genome is composed of hundreds of families of transposable elements, dispersed nonuniformly across the genome. These were responsible for the capture and amplification of numerous gene fragments and affect the composition, sizes, and positions of centromeres. We also report on the correlation of methylation-poor regions with Mu transposon insertions and recombination, and copy number variants with insertions and/or deletions, as well as how uneven gene losses between duplicated regions were involved in returning an ancient allotetraploid to a genetically diploid state. These analyses inform and set the stage for further investigations to improve our understanding of the domestication and agricultural improvements of maize.
Critical area based yield prediction using in-line defect classification information [DRAMs] (2002)

Optically measured in-line defect data is used for critical area analysis based yield prediction. Because this data can be noisy, however, data can be filtered using kill ratios established from in-line defect to bitmap correlation by mask layer on arrayed devices. This paper reports results from increased granularity of the kill ratio analysis: in-line defect classifications are considered and individual kill ratios for each classification are calculated and used for yield modeling. Furthermore, performing automatic signature classification on the bitmaps and signature to defect correlation adds valuable insight into yield loss mechanisms and improves the accuracy of the yield model.
69.3: Invited Paper : Advanced MVA for High Quality LCD‐TVs (2006)

Abstract We proposed an advanced MVA, AMVA (Advanced MVA or AUO MVA), which has the features required by an advanced LCD‐TV. AMVA has the true wide viewing angle, high color saturation and fast response speed for motion picture displaying. Besides, the image processing techniques to enhance the image performance with vivid color and sharpness are also obtained. AMVA has become the promising solution of advanced LCD‐TV panels for mass‐production.
Expression of the Extra Domain B of Fibronectin, a Marker of Angiogenesis, in Head and Neck Tumors (2003)

The results show that ED-B is abundantly expressed around the neovasculature and in the stroma of the majority of malignant tumors of the head and neck but is undetectable in normal tissues. The ED-B domain of fibronectin is a good-quality tumor-stroma-associated antigen that warrants clinical trials with antibody-based pharmaceuticals, including immunoscintigraphic investigations and radioimmunoguided surgery with the radiolabeled L19 antibody.
PAMAM Dendrimers and Branched Polyethyleneglycol (Nanoparticles) Prodrugs of (-)-β-D-(2R, 4R)-dioxolane-thymine (DOT) and Their Anti-HIV Activity (2006)

The synthesis, characterization, anti-HIV activity and cytotoxicity of dendrimers of (-)-beta-D-(2R, 4R)-dioxolane-thymine (DOT) and polyethylene glycol (PEG)-DOT conjugates are described. Dendrimers in this study were polyamidoamine (PAMAM) generation 2.0, 3.0, 5.0 and 6.0, along with 8.0-branched PEG with a molecular weight of 40 kDa. DOT was attached to PAMAM dendrimers or branched PEG via ester or phosphafte groups. Size exclusion chromatography was used to purify the dendrimers and PEG conjugates, which were characterized by NMR and MALDI-TOF mass spectrometry. The synthesized PAMAM dendrimers and PEG conjugates were evaluated for anti-HIV activity against HIV-1LAI in primary human peripheral blood mononuclear cells (PBMCs) and cytotoxicity in PBMCs, CEM and Vero cells. PAMAM dendrimers of DOT with ester linkages and particularly phosphate linkers showed an increase in anti-HIV potency in comparison with DOT alone (140- and 56-fold, respectively). Unfortunately, the PAMAM dendrimers also exhibited increased cytotoxicity. Anti-HIV activity of PEG-DOT conjugates was found to be lower than that of DOT.
Adherence of group B streptococci to adult and neonatal epithelial cells mediated by lipoteichoic acid (1987)

We have investigated the role of lipoteichoic acid in mediating the adherence of different serotypes of group B streptococci to human adult and neonatal epithelial cells. Pretreatment of neonatal buccal and vaginal epithelial cells with lipoteichoic acid, but not with deacylated lipoteichoic acid, induced a marked inhibition in the adherence of all strains tested. Pretreatment of bacteria with substances known to bind lipoteichoic acid, such as monoclonal and polyclonal antipolyglycerophosphate antibodies and albumin, also resulted in adherence inhibition. Group B streptococci adhered in 6- to 10-fold-higher numbers to buccal epithelial cells from neonates older than 3 days than to those from neonates less than 1 day old. This increase in receptiveness for group B streptococci was paralleled by an increased ability of epithelial cells from older neonates to bind group B streptococcal lipoteichoic acid. These data suggest a role for the lipid portion of lipoteichoic acid in the adherence of different serotypes of group B streptococci to vaginal and neonatal epithelial cells.
The Emerging Role of MicroRNAs in Cardiac Remodeling and Heart Failure (2008)

Recent studies have suggested a potentially important role for a family of tiny regulatory RNAs, known as microRNAs (miRNAs or miRs), in the control of diverse aspects of cardiac function in health and disease. Although the field of miRNA biology is relatively new, there is emerging evidence that miRNAs may play an important role in the pathogenesis of heart failure through their ability to regulate the expression levels of genes that govern the process of adaptive and maladaptive cardiac remodeling. Here, we review the biology of miRNAs in relation to their role in modulating various aspects of the process of cardiac remodeling, as well as discuss the potential application of miRNA biology to the field of heart failure.
Effectiveness and Safety of Transcranial Laser Therapy for Acute Ischemic Stroke (2009)

TLT within 24 hours from stroke onset demonstrated safety but did not meet formal statistical significance for efficacy. However, all predefined analyses showed a favorable trend, consistent with the previous clinical trial (NEST-1). Both studies indicate that mortality and adverse event rates were not adversely affected by TLT. A definitive trial with refined baseline National Institutes of Health Stroke Scale exclusion criteria is planned.
MicroRNA as biomarkers and regulators in B-cell chronic lymphocytic leukemia (2011)

Early cancer detection and disease stratification or classification are critical to successful treatment. Accessible, reliable, and informative cancer biomarkers can be medically valuable and can provide some relevant insights into cancer biology. Recent studies have suggested improvements in detecting malignancies by the use of specific extracellular microRNAs (miRNAs) in plasma. In chronic lymphocytic leukemia (CLL), an incurable hematologic disorder, sensitive, early, and noninvasive diagnosis and better disease classification would be very useful for more effective therapies. We show here that circulating miRNAs can be sensitive biomarkers for CLL, because certain extracellular miRNAs are present in CLL patient plasma at levels significantly different from healthy controls and from patients affected by other hematologic malignancies. The levels of several of these circulating miRNAs also displayed significant differences between zeta-associated protein 70 (ZAP-70)(+) and ZAP-70(-) CLL. We also determined that the level of circulating miR-20a correlates reliably with diagnosis-to-treatment time. Network analysis of our data, suggests a regulatory network associated with BCL2 and ZAP-70 expression in CLL. This hypothesis suggests the possibility of using the levels of specific miRNAs in plasma to detect CLL and to determine the ZAP-70 status.
Prominent infectious diseases expert to open Virginia Tech deans' forum (2008)

The scientist appointed to lead a major new Centers for Disease Control and Prevention initiative to combat infectious diseases will present the keynote address during Virginia Tech's upcoming Deans' Forum on Infectious Diseases.
Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway (2016)

In this study, S. cerevisiae was engineered for high-level production of 3HP by increasing the copy numbers of biosynthetic genes and improving flux towards precursors and redox cofactors. This strain represents a good platform for further optimization of 3HP production and hence an important step towards potential commercial bio-based production of 3HP.
Serum Total Carbon Dioxide (1990)

We measure serum total CO2 content in lieu of measuring serum bicarbonate. The total CO2 content includes the serum bicarbonate as well as available forms of carbon dioxide (i.e., dissolved CO2 and carbonic acid). Generally, the serum bicarbonate comprises about 95% of the total CO2 content; thus we can use this measurement as an excellent estimator of serum bicarbonate. The total CO2 content normally equals 23 to 30 mEq/L of serum.
Visió retrospectiva de la problemàtica hipotecària (2013)

Amb motiu de la promulgacio del Reial Decret-Llei 27/2012 de 15 de novembre, de mesures urgents per a reforcar la proteccio als deutors hipotecaris, la comissio de normativa de l'ICAB va organitzar una jornada que, coordinada per Jesus Sanchez, diputat responsable de la citada comissio, va comptar amb la intervencio dels magistrats Guillem Soler Sole i Gemma Vives Martinez; els professors Marti Batllori Bas i Vicente Perez Daudi i Marta Legarreta Fontelles, advocada i vocal de la seccio de dret civil. Us oferim tot seguit el resum de les ponencies dels dos magistrats, Guillem Soler i Gemma Vives.
Application of annihilation coincidence detection to transaxial reconstruction tomography. (1975)

A study was carried out to investigate the use of annihilation coincidence detection (ACD) in emmision transaxial reconstruction tomography. The ACD was evaluated in terms of spatial resolution and sensitivity with depth, detection efficiency, effect of pulse-height analysis on resolution and efficiency, correction for attenuation, and cold spot contrast. A prototype positron emission transaxial tomograph (PETT) consisting of a hexagonal array of 24 Nal (Tl) detectors employing ACD was constructed. A fast Fourier transform algorithm was employed to generate the reconstructed image. Computer simulations and phantom and animal studies were carried out to demonstrate that this approach yields tomographic radionuclide images that have high resolution and contrast (hot and cold spot) and that are independent of activity above and below the plane examined. The ACD yields a quantitative nuclear medicine imaging device with high detection efficiency. Comparisons are presented between the ACD and the scintillation camera and scanner. Discussion of the possible applications of the PETT in nuclear medicine is included.
The Second Golden Age of Glycomics: From Functional Glycomics to Clinical Applications (2009)

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTThe Second Golden Age of Glycomics: From Functional Glycomics to Clinical ApplicationsNaoyuki Taniguchi, William Hancock, David M. Lubman, and Pauline M. RuddView Author Information Endowed Chair Professor, Osaka University, Program Director, RIKEN Advanced Science Institute (Japan) Professor of Chemistry and Bradstreet Chair in Bioanalytical Chemistry, Barnett Institute of Chemical and Biological Analysis and Northeastern University Maude T. Lane Professor of Surgical Immunology, University of Michigan Medical Center NIBRT Professor of Glycobiology, National Institute for Bioprocessing Research and Training, Dublin−Oxford Glycobiology Laboratory, Conway Institute, and University College DublinCite this: J. Proteome Res. 2009, 8, 2, 425–426Publication Date (Web):January 9, 2009Publication History Received9 December 2008Published online9 January 2009Published inissue 6 February 2009https://doi.org/10.1021/pr801057jCopyright © 2009 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views2986Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (109 KB) Get e-AlertscloseSUBJECTS:Bioanalytical chemistry,Cancer,Carbohydrates,Chemical biology,Peptides and proteins Get e-Alerts
Different membrane anchors allow the Semliki Forest virus spike subunit E2 to reach the cell surface (1985)

The Semliki Forest virus spike subunit E2, a membrane-spanning protein, was transported to the plasma membrane in BHK cells after its carboxy terminus, including the intramembranous and cytoplasmic portions, was replaced by respective fragments of either the vesicular stomatitis virus glycoprotein or the fowl plague virus hemagglutinin. The hybrid proteins were constructed by cDNA fusion. Upon a transient expression they could be localized at the cell surface by immunofluorescence with specific antibodies directed against any of the protein fragments.
Politeness : Some Universals in Language Usage (1987)

This study is about the principles for constructing polite speech. The core of it was published as Brown and Levinson (1978); here it is reissued with a new introduction which surveys the now considerable literature in linguistics, psychology and the social sciences that the original extended essay stimulated, and suggests new directions for research. We describe and account for some remarkable parallelisms in the linguistic construction of utterances with which people express themselves in different languges and cultures. A motive for these parallels is isolated - politeness, broadly defined to include both polite friendliness and polite formality - and a universal model is constructed outlining the abstract principles underlying polite usages. This is based on the detailed study of three unrelated languages and cultures: the Tamil of south India, the Tzeltal spoken by Mayan Indians in Chiapas, Mexico, and the English of the USA and England, supplemented by examples from other cultures. Of general interest is the point that underneath the apparent diversity of polite behaviour in different societies lie some general pan-human principles of social interaction, and the model of politeness provides a tool for analysing the quality of social relations in any society.
Doxorubicin, Cardiac Risk Factors, and Cardiac Toxicity in Elderly Patients With Diffuse B-Cell Non-Hodgkin's Lymphoma (2008)

Among patients receiving chemotherapy for DLBCL, those with prior heart disease were less likely than others to be treated with doxorubicin, and those who received doxorubicin were more likely than others to develop CHF. Various cardiac risk factors increased CHF risk, but only hypertension was synergistic with doxorubicin. Doxorubicin has dramatically improved survival of DLBCL patients; nonetheless, some subgroups may benefit from efforts to reduce doxorubicin-related CHF risk.
Generation of 1E8 Single Chain Fv-Fc Construct Against Human CD59 (2012)

A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.
Palladium-catalyzed asymmetric allylic alkylation using dimethyl malonate and its derivatives as nucleophile (1991)

Palladlum-catalyzed asymmetric allylic alkylatlon of 1,3-dlphenyl-2-propenyl acetate with sodium salt of dimethyl malonate and its derivatives has been successfully carried out in the presence of optically active diphosphine, such as (S)-BINAP. High enantioselectivity of up to 90% ee was obtained with dimethyl malonate.