gebhart/patrepeval huggingface dataset:
Characterization, Sequencing, and Expression of the Genes Encoding a Reactivating Factor for Glycerol-inactivated Adenosylcobalamin-dependent Diol Dehydratase (1997)

Diol dehydratase undergoes suicide inactivation by glycerol during catalysis involving irreversible cleavage of the Co-C bond of adenosylcobalamin. In permeabilized Klebsiella oxytoca and Klebsiella pneumoniae cells, the glycerol-inactivated holoenzyme or the enzyme-cyanocobalamin complex is rapidly activated by the exchange of the inactivated coenzyme or cyanocobalamin for free adenosylcobalamin in the presence of ATP and Mg2+ (Honda, S., Toraya, T., and Fukui, S. (1980) J. Bacteriol. 143, 1458-1465; Ushio, K., Honda, S., Toraya, T., and Fukui, S. (1982) J. Nutr. Sci. Vitaminol. 28, 225-236). Permeabilized Escherichia coli cells co-expressing the diol dehydratase genes with two open reading frames in the 3'-flanking region were capable of reactivating glycerol-inactivated diol dehydratase as well as activating the enzyme-cyanocobalamin complex in situ in the presence of free adenosylcobalamin, ATP, and Mg2+. These open reading frames, designated as ddrA and ddrB genes, were identified as the genes of a putative reactivating factor for inactivated diol dehydratase. The genes encoded polypeptides consisting of 610 and 125 amino acid residues with predicted molecular weights of 64,266 and 13,620, respectively. Co-expression of the open reading frame in the 5'-flanking region was stimulatory but not obligatory for conferring the reactivating activity upon E. coli. Thus, the product of this gene was considered not an essential component of the reactivating factor.
Analysis of consecutive droughts on the Canadian Prairies (2006)

Canadian Prairie precipitation totals were classified using fixed thresholds corresponding to the 10th percentile and median for the growing season (April to August) and agricultural year (September to August), in order to study the incidence of consecutive drought. These time frames are significant to water supply and agricultural activities. Consecutive drought events were classified as either back to back years with precipitation below the 10th percentile, or a year with precipitation below the 10th percentile followed by one or more years with less than median precipitation. The Palliser Triangle (an area in the southern Prairies) most consistently recorded consecutive drought events. While generally receiving higher precipitation totals, stations in the north were not immune to such events. Using the Palmer Drought Index, severe droughts were detected in the 1910s, 1930s and 1960s, corresponding to notably dry periods across the Canadian Prairies as a whole.
Solubilization and Characterization of Prostaglandin E2 Binding Protein from Porcine Cerebral Cortex (1986)

The specific binding protein for prostaglandin (PG) E2 was solubilized in an active form from the crude mitochondrial (P2) fraction of porcine cerebral cortex. After incubation with 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS) at 4 degree C for 30 min, the PGE2 binding to the supernatant fraction (103,000 g, 60 min) was determined by the polyethylene glycol method. The maximum yield (approximately 30% of the binding activity to the P2 fraction) was obtained with 10 mM CHAPS. The specific [3H]PGE2 binding to the solubilized fraction was time-dependent and the equilibrium was reached at around 60 min at 37 degrees C. By dilution of the reaction mixture, the binding site-[3H]PGE2 complex formed after 5-min incubation slowly dissociated, whereas that formed after 60-min incubation did not dissociate to a significant extent. The binding was highly specific for PGE2 and inhibited by unlabeled PGs in the following order: PGE2 greater than PGE1 much greater than PGF2 alpha greater than PGE2 methyl ester greater than PGA2 greater than 13,14-dihydro-15-keto-PGE2 greater than PGD2. Scatchard analyses of the solubilized fraction suggested the presence of high- and low-affinity sites. Heat treatment and preincubation with trypsin or proteinase K markedly reduced the binding. The binding activity was eluted in a single peak both from gel filtration and from ion-exchange columns using HPLC. These results suggest that a specific protein solubilized may be responsible for the binding site.
Boron in the Environment (2005)

Boron has recently come to the attention of the U.S. Environmental Protection Agency as a possible contaminant worthy of regulation, but questions must still be addressed before a regulatory determination can take place. This article reviews current knowledge about boron as it pertains to water treatment and the environment so that informed decisions can be made regarding regulations and direction of future research. Specifically, this article summarizes boron chemistry, boron occurrence, health effects of boron, existing guidelines and regulations concerning boron, and methods for removing boron from drinking water.
Long-Term Survival Outcomes with Intravesical Docetaxel for Recurrent Nonmuscle Invasive Bladder Cancer After Previous Bacillus Calmette-Guérin Therapy (2012)

Intravesical docetaxel appears to be a promising agent with significant efficacy and durability for bacillus Calmette-Guérin refractory nonmuscle invasive bladder cancer. Adding maintenance treatments may increase the duration of recurrence-free survival.
Enhanced Aggregation of Androgen Receptor in Induced Pluripotent Stem Cell-derived Neurons from Spinal and Bulbar Muscular Atrophy (2013)

Spinal and bulbar muscular atrophy (SBMA) is an X-linked motor neuron disease caused by a CAG repeat expansion in the androgen receptor (AR) gene. Ligand-dependent nuclear accumulation of mutant AR protein is a critical characteristic of the pathogenesis of SBMA. SBMA has been modeled in AR-overexpressing animals, but precisely how the polyglutamine (polyQ) expansion leads to neurodegeneration is unclear. Induced pluripotent stem cells (iPSCs) are a new technology that can be used to model human diseases, study pathogenic mechanisms, and develop novel drugs. We established SBMA patient-derived iPSCs, investigated their cellular biochemical characteristics, and found that SBMA-iPSCs can differentiate into motor neurons. The CAG repeat numbers in the AR gene of SBMA-iPSCs and also in the atrophin-1 gene of iPSCs derived from another polyQ disease, dentato-rubro-pallido-luysian atrophy (DRPLA), remain unchanged during reprogramming, long term passage, and differentiation, indicating that polyQ disease-associated CAG repeats are stable during maintenance of iPSCs. The level of AR expression is up-regulated by neuronal differentiation and treatment with the AR ligand dihydrotestosterone. Filter retardation assays indicated that aggregation of ARs following dihydrotestosterone treatment in neurons derived from SBMA-iPSCs increases significantly compared with neurological control iPSCs, easily recapitulating the pathological feature of mutant ARs in SBMA-iPSCs. This phenomenon was not observed in iPSCs and fibroblasts, thereby showing the neuron-dominant phenotype of this disease. Furthermore, the HSP90 inhibitor 17-allylaminogeldanamycin sharply decreased the level of aggregated AR in neurons derived from SBMA-iPSCs, indicating a potential for discovery and validation of candidate drugs. We found that SBMA-iPSCs possess disease-specific biochemical features and could thus open new avenues of research into not only SBMA, but also other polyglutamine diseases.
Temporal analysis of pathogenic events in virulent and avirulent Brucella melitensis infections (2005)

Despite progress in mouse models of brucellosis, much remains unknown regarding Brucella dissemination and tissue localization. Here, we report the dynamics of Brucella infection in individual mice using bioluminescent Brucella melitensis. Bioluminescent imaging of infected interferon regulatory factor-1 knockout (IRF-1–/–) mice identified acute infection in many tissues. Brucella was found to replicate in the salivary glands of IRF-1–/– and wild-type C57BL/6 mice suggesting a previously unknown tissue preference. Establishing a niche in this region may have relevance in humans where infection can result from ingestion of few bacteria. Sublethal infection of IRF-1–/– mice resulted in chronic Brucella localization in tail joints, an infection parallel to osteoarticular brucellosis in humans. Importantly, bioluminescent imaging rapidly identified attenuated EZ::TN/lux mutants in infected mice and revealed differences in dissemination, thereby defining the contribution of Brucella genes to virulence and tissue localization. Surprisingly, a virB mutant, though defective in persistence, disseminated similarly to virulent Brucella, suggesting bacterial spread is independent of VirB proteins that are important for intracellular survival. Together, our results reveal kinetics of acute and chronic Brucella infection in individual mice that parallels human infection as well as readily identified attenuated bacteria. Our approach facilitates identifying virulence determinants that may control tissue specific replication and may help develop therapeutics to overcome Brucella-induced chronic debilitating conditions.
On generating FC/sup 3/ fuzzy rule systems from data using evolution strategies (1999)

Sophisticated fuzzy rule systems are supposed to be flexible, complete, consistent and compact (FC(3)). Flexibility, and consistency are essential for fuzzy systems to exhibit an excellent performance and to have a clear physical meaning, while compactness is crucial when the number of the input variables increases. However, the completeness and consistency conditions are often violated if a fuzzy system is generated from data collected from real world applications. A systematic design paradigm is proposed using evolution strategies. The structure of the fuzzy rules, which determines the compactness of the fuzzy systems, is evolved along with the parameters of the fuzzy systems. Special attention has been paid to the completeness and consistency of the rule base. The completeness is guaranteed by checking the completeness of the fuzzy partitioning of input variables and the completeness of the rule structure. An index of inconsistency is suggested with the help of a fuzzy similarity which can prevent the algorithm from generating rules that seriously contradict with each other or with the heuristic knowledge. In addition, soft T-norm and BADD defuzzification are introduced and optimized to increase the flexibility of the fuzzy system. The proposed approach is applied to the design of a distance controller for cars. It is verified that a FC(3) fuzzy system works very well both, for training and test driving situations, especially when the training data are insufficient.
Insertion Loss and Crosstalk Analysis of a Fiber Switch Based on a Pixelized Phase Modulator (2010)

We analyze the performance of a spatial fiber switching system when using a pixelized mirror, such as a LCoS or MEMS spatial light modulator, in place of a large tilting micromirror. Our findings demonstrate the dependence of insertion losses on tilt angles or fiber counts, and the dependence of the crosstalk in the number of phase quantization levels and random phase errors. The former effects can be minimized by satisfying a relationship between the tilt angle to a fiber, the pitch of the array, and the optical wavelength.
Identifying Potential Tumor Markers and Antigens by Database Mining and Rapid Expression Screening (2000)

Genes expressed specifically in malignant tissue may have potential as therapeutic targets but have been difficult to locate for most cancers. The information hidden within certain public databases can reveal RNA transcripts specifically expressed in transformed tissue. To be useful, database information must be verified and a more complete pattern of tissue expression must be demonstrated. We tested database mining plus rapid screening by fluorescent-PCR expression comparison (F-PEC) as an approach to locate candidate brain tumor antigens. Cancer Genome Anatomy Project (CGAP) data was mined for genes highly expressed in glioblastoma multiforme. From 13 mined genes, seven showed potential as possible tumor markers or antigens as determined by further expression profiling. Now that large-scale expression information is readily available for many of the commonly occurring cancers, other candidate tumor markers or antigens could be located and evaluated with this approach.
Methylene chloride-induced tumorigenesis (1993)

Journal Article Methylene chloride-induced tumorigenesis Get access M. W. Anderson, M. W. Anderson Search for other works by this author on: Oxford Academic PubMed Google Scholar R. R. Maronpot R. R. Maronpot Search for other works by this author on: Oxford Academic PubMed Google Scholar Carcinogenesis, Volume 14, Issue 5, May 1993, Pages 787–788, https://doi.org/10.1093/carcin/14.5.787 Published: 01 May 1993
Development of the antimicrobial effectiveness test as USP chapter <51>. (2003)

The antimicrobial effectiveness test first appeared as a USP General Chapter in the 18th revision, official September 1, 1970. This chapter, at the beginning, was designed to evaluate the performance of antimicrobials added to inhibit the growth of microorganisms that might be introduced during or subsequent to the manufacturing process. As Good Manufacturing Practices (GMPs) became a governing principal in pharmaceutical manufacturing, the purpose of the test was refined to focus on activity of the preservative system as a protection against inadvertent contamination during storage and usage of the product. This article will review the history of the antimicrobial test; its function, technique, and the background discussions that resulted in the changes from the test that appeared in USP XVIII to that of the current USP 25.
Cytokines in synovial fluid. I. The presence of biologically active and immunoreactive IL-1. (1988)

Using a sensitive IL-1-dependent T cell clone we estimated interleukin 1 (IL-1)-like activity in a variety of synovial fluids (SF). Although we demonstrate that SF contains potent inhibitors of endogenous and exogenous IL-1 we were able to estimate that samples from rheumatoid arthritis, osteoarthritis and other arthritides contain the equivalent of tens or hundreds of picograms of IL-1 per millilitre, when compared to a standard IL-1 preparation. Although there was some correlation between these results and those obtained using a radio-immunoassay (RIA) for IL-1 beta, the RIA indicated that IL-1 was present in the nanogram range. The results suggest that the importance of inhibitors of IL-1 in SF merits particular attention and that it is also important to consider the bioactivity of cytokines which may be detected by physical methods.
Trastuzumab distribution in an in-vivo and in-vitro model of brain metastases of breast cancer (2017)

This study is one of the first studies to measure antibody movement across the blood-brain and blood-tumor barriers, and demonstrates that, though in small and most likely not efficacious quantities, trastuzumab does cross the blood-brain and blood-tumor barriers.
A Power-Scalable Channel-Adaptive Wireless Receiver Based on Built-In Orthogonally Tunable LNA (2012)

Most of the traditional RF circuits are static or minimally tunable using some digital controllability. For example, high power, low power and shut down modes are available in some commercially available transceivers. If available, the tuning knobs affect different specifications in an interdependent manner, resulting in changing several specifications when only one needs to be tuned. This is not enough for power optimal operation of complete self-aware self-adaptive circuits and systems. Independent or orthogonal tunability of the important conflicting specifications using built-in tuning knobs allow optimal adaptation of the wire-less systems. In this paper we demonstrate the design and benefits of orthogonal tuning knobs using an inductorless LNA as a test vehicle. The design of an orthogonally tunable LNA is discussed that has a 14 dB Gain tuning range and 30 dB OIP3 tuning range as its power consumption goes down by 20×. Measurement results on this LNA validate the concept of orthogonal tunability. Use of this orthogonally tunable LNA within an adaptive wireless receiver framework shows power savings of 2× compared to a static system and an extra savings of 22% compared to a traditional nonorthogonal adaptive system.
Jeremiah. Volume I. (The Daily Study Bible.) By Robert Davidson. Edinburgh, The St. Andrew Press, 1983. Pp. 165. £2·95. - Psalms. Volume II. (The Daily Study Bible.) By G. A. F. Knight. Edinburgh, The St. Andrew Press, 1983. Pp. 368. £2·95. (1985)

Jeremiah. Volume I. (The Daily Study Bible.) By Robert Davidson. Edinburgh, The St. Andrew Press, 1983. Pp. 165. £2·95. - Psalms. Volume II. (The Daily Study Bible.) By G. A. F. Knight. Edinburgh, The St. Andrew Press, 1983. Pp. 368. £2·95. - Volume 38 Issue 1
(Invited) Success in Measurement the Lowest Off-state Current of Transistor in the World (2011)

An oxide semiconductor has a wide band gap and low off-state current. We focused on the off-state characteristics of a transistor including an oxide semiconductor and concentrated on measuring the off-state current. We fabricated a circuit TEG that had an In-Ga-Zn-Oxide TFT (IGZO-TFT) with a large channel width of 100,000 micro meter over a glass substrate and estimated the amount of electric charge flowing through the circuit TEG for a long time. As a result, we discovered that an IGZO-TFT had off-state current as small as 50yA/micro meter (at 85 degrees of Centigrade). We are the first to measure off-state current on the order of yA; "y" is the SI prefix representing 10e-24
Both the epitope specificity and isotype are important in the antitumor effect of monoclonal antibodies against Her‐2/neu antigen (2002)

The Her-2/neu oncogene, which encodes a growth factor receptor, was implicated in the malignancy of human adenocarcinomas. Antibodies directed to this molecule have been previously shown to have an antitumor effect in vivo. In an attempt to understand the mechanisms of the antitumor activity, we generated 2 monoclonal antibodies (mAbs), HRO G1 and HRT G1, that recognize different epitopes on Her-2/neu. Both of the mAbs bound HER2/neu on the tumor surface, resulting in phosphorylation of HER2/neu. We also generated IgG2a and IgG2b mAbs from these 2 mAbs, respectively. The results of in vitro studies showed that these anti-Her-2/neu mAbs could not inhibit the growth of the tumor cells that express Her-2/neu molecules by themselves. However, in an antibody-dependent cellular cytotoxicity study using mouse splenocytes as effector cells, HRT mAbs had antitumor activities superior to those of HRO mAbs, indicating that the epitope specificity may also partake in antibody-dependent cellular cytotoxicity with antibody isotype. In a complement-dependent cytotoxicity study, the IgG2a and IgG2b mAbs showed stronger effects than IgG1 isotype mAbs irrespective of the epitope specificities. The results of in vivo studies also showed that HRT mAbs had superior antitumor activity to those of HRO mAbs. The antitumor activity was most prominent in the HRT G2b isotype among HRT mAbs. HRT G1 also showed a moderate antitumor effect, while HRT G2a showed only slight inhibition effect. These data indicate that both the epitope specificity and the differences in Fc region of mAbs could play important roles in the antitumor activities.
Poly(Vinyl Pyridine) as a Universal Surface Modifier for Immobilization of Nanoparticles (2002)

Metal, metal oxide, and plastic surfaces were modified with poly(vinylpyridine) using adsorption, spin-coating, and dip-coating techniques and various metal, semiconductor, and dielectric nanoparticles were subsequently attached. Prior to the modification, the substrates were treated with glow discharge plasma for cleaning purposes and to introduce surface functional groups that are capable of interaction with the polymer. UV−vis absorption spectroscopy, atomic force, and electron scanning microscopies were used to characterize polymer layers and layers of immobilized nanoparticles. The surface modification with poly(vinylpyridine) via adsorption technique is suggested as a simple, effective, and highly versatile method for the preparation of single, mixed, and multilayered assemblies of various nanoparticles on different substrates.
Load balancing and fault tolerance in workstation clusters migrating groups of communicating processes (1995)

In the past, several process migration facilities for distributed systems have been developed. Due to the complex nature of the subject, all those facilities have limitations that make them usable for only limited classes of applications and environments. We discuss some of the usual limitations and possible solutions. Specifically, we focus on migration of groups of collaborating processes between Unix systems without kernel modifications, and from this we derive the design for a migration system. First experiences with our implementation show that we reach performance figures for the migration that are close to those of real distributed operating system.
Chest Pulse-Wave Velocity: A Novel Approach to Assess Arterial Stiffness (2010)

Pulse-wave velocity (PWV) is considered as the gold-standard method to assess arterial stiffness, an independent predictor of cardiovascular morbidity and mortality. Current available devices that measure PWV need to be operated by skilled medical staff, thus, reducing the potential use of PWV in the ambulatory setting. In this paper, we present a new technique allowing continuous, unsupervised measurements of pulse transit times (PTT) in central arteries by means of a chest sensor. This technique relies on measuring the propagation time of pressure pulses from their genesis in the left ventricle to their later arrival at the cutaneous vasculature on the sternum. Combined thoracic impedance cardiography and phonocardiography are used to detect the opening of the aortic valve, from which a pre-ejection period (PEP) value is estimated. Multichannel reflective photoplethysmography at the sternum is used to detect the distal pulse-arrival time (PAT). A PTT value is then calculated as PTT = PAT - PEP. After optimizing the parameters of the chest PTT calculation algorithm on a nine-subject cohort, a prospective validation study involving 31 normo- and hypertensive subjects was performed. 1/chest PTT correlated very well with the COMPLIOR carotid to femoral PWV (r = 0.88, p < 10 (-9)). Finally, an empirical method to map chest PTT values onto chest PWV values is explored.