gebhart/patrepeval huggingface dataset:
Open Access Integrated Therapeutic and Diagnostic Platforms for Personalized Cardiovascular Medicine (2013)

It is undeniable that the increasing costs in healthcare are a concern. Although technological advancements have been made in healthcare systems, the return on investment made by governments and payers has been poor. The current model of care is unsustainable and is due for an upgrade. In developed nations, a law of diminishing returns has been noted in population health standards, whilst in the developing world, westernized chronic illnesses, such as diabetes and cardiovascular disease have become emerging problems. The reasons for these trends are complex, multifactorial and not easily reversed. Personalized medicine has the potential to have a significant impact on these issues, but for it to be truly successful, interdisciplinary mass collaboration is required. We propose here a vision for open-access advanced analytics for personalized cardiac diagnostics using imaging, electrocardiography and genomics.
Fas Signalling Promotes Intercellular Communication in T Cells (2012)

Cell-to-cell communication is a fundamental process for development and maintenance of multicellular organisms. Diverse mechanisms for the exchange of molecular information between cells have been documented, such as the exchange of membrane fragments (trogocytosis), formation of tunneling nanotubes (TNTs) and release of microvesicles (MVs). In this study we assign to Fas signalling a pivotal role for intercellular communication in CD4+ T cells. Binding of membrane-bound FasL to Fas expressing target cells triggers a well-characterized pro-apoptotic signalling cascade. However, our results, pairing up flow cytometric studies with confocal microscopy data, highlight a new social dimension for Fas/FasL interactions between CD4+ T cells. Indeed, FasL enhances the formation of cell conjugates (8 fold of increase) in an early time-frame of stimulation (30 min), and this phenomenon appears to be a crucial step to prime intercellular communication. Our findings show that this communication mainly proceeds along a cytosolic material exchange (ratio of exchange >10, calculated as ratio of stimulated cells signal divided by that recorded in control cells) via TNTs and MVs release. In particular, inhibition of TNTs genesis by pharmacological agents (Latruculin A and Nocodazole) markedly reduced this exchange (inhibition percentage: >40% and >50% respectively), suggesting a key role for TNTs in CD4+ T cells communication. Although MVs are present in supernatants from PHA-activated T cells, Fas treatment also leads to a significant increase in the amount of released MVs. In fact, the co-culture performed between MVs and untreated cells highlights a higher presence of MVs in the medium (1.4 fold of increase) and a significant MVs uptake (6 fold of increase) by untreated T lymphocytes. We conclude that Fas signalling induces intercellular communication in CD4+ T cells by different mechanisms that seem to start concomitantly with the main pathway (programmed cell death) promoted by FasL.
Performance analysis of an echo-cancellation arrangement that compensates for frequency offset in the far echo (1988)

Analytical and experimental results are presented for the performance of one echo canceller arrangement. It consists of a data-driven echo canceller having a so-called cross-coupled structure, which is followed by a rotator and a phase-locked loop (PLL). A cross-coupled echo canceller structure without a PLL is analyzed first. Expressions for speed of convergence and achievable echo-return-loss enhancement (ERLE) in the presence of frequency offset are derived. These results are compared in previously published results for a noncross-coupling echo canceller structure. Specifically, it is shown that the cross-coupled structure converges twice as fast as the noncross-coupled structure and provide an achievable ERLE that is about 6 dB better. The joint adaptation of the echo canceller and the PLL is then studied. It is shown that it is always possible to choose design parameters for the echo canceller which are consistent with adaptation requirements under double-talking conditions, provided that the PLL is properly engineered. The sensitivity of the performance of PLL to the power level of the far echo, as well as possible solutions to this problem, are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
High-energy single-transverse-mode Q-switched fiber laser based on a multimode large-mode-area erbium-doped fiber (1998)

We demonstrate that appropriately designed doped multimode fibers provide robust single-mode output when used within a fiber laser cavity. Using a novel large-mode-area fiber, we demonstrate what we believe to be record single-mode (M(2) <1.2) pulse energies of >0.5 mJ from a Q -switched fiber laser and even higher pulse energies (as high as 0.85 mJ) with slightly compromised spatial-mode quality (M(2)<2.0) . This approach offers significant scope for extending the range of single-mode output powers and energies that are achievable from fiber-laser-amplifier systems.
Embedded Star Formation and Circumstellar Disk Evolution: Early Results from MIPS (2006)

We present some recent results from the Guaranteed Time Young Clusters program, with particular emphasis on results from the MIPS instrument. In this program we have surveyed some of the best examples of nearby clusters using both IRAC and MIPS. The clusters range in age from less than 1 Myr to over 100 Myr, and they represent a variety of environments with regard to the presence of massive stars. In the youngest clusters, the MIPS observations show star formation occuring on large scales in coherent structures that trace out the dense gas. In the older clusters in our sample, the circumstellar material has disappeared around most of the stars, but a small fraction of the sources show evidence for debris disks.
Activation of DNA Methyltransferase 1 by EBV Latent Membrane Protein 2A Leads to Promoter Hypermethylation of PTEN Gene in Gastric Carcinoma (2009)

CpG island promoter methylation of tumor suppressor genes is one of the most characteristic abnormalities in EBV-associated gastric carcinoma (GC). Aberrant promoter methylation and expression loss of PTEN were evaluated in cancer tissues of GC by methylation-specific PCR and immunohistochemistry, respectively, showing that both abnormalities occurred concurrently in EBV-associated GC. PTEN abnormalities were reiterated in GC cell lines MKN-1 and MKN-7 infected with recombinant EBV, and DNA methyltransferase 1 (DNMT1) was commonly overexpressed in both cell lines. Stable and transient transfection systems in MKN-1 similarly showed that viral latent membrane protein 2A (LMP2A) up-regulated DNMT1, leading to an increase in methylation of the PTEN promoter. Importantly, the level of phosphorylated signal transducer and activator of transcription 3 (pSTAT3) increased in the nuclei of LMP2A-expressing GC cells, and knockdown of STAT3 counteracted LMP2A-mediated DNMT1 overexpression. Immunohistochemistry for both pSTAT3 and DNMT1 showed diffuse labeling in the nuclei of the cancer cells in GC tissues, especially in EBV-associated GC. Taken together, LMP2A induces the phosphorylation of STAT3, which activates DNMT1 transcription and causes PTEN expression loss through CpG island methylation of the PTEN promoter in EBV-associated GC. LMP2A plays an essential role in the epigenetic abnormalities in host stomach cells and in the development and maintenance of EBV-associated cancer.
Wounding alters epidermal connexin expression and gap junction-mediated intercellular communication. (1995)

We show that connexin expression and in vivo patterns of communication were dramatically altered in response to epidermal wounding. Six hours after injury, Cx26 was up-regulated in the differentiated cells proximal to the wound, but was down-regulated in cells located at the wound edge. In contrast, Cx31.1 and Cx43 were down-regulated in cells both peripheral to and at the wounded edge. These patterns of altered connexin expression were detectable as early as 2 h after wounding and were most pronounced in 24-h old wounds. Increased expression of Cx26 was still evident in the hyperproliferative epidermis of 6-day old wounds. In vivo dye transfer experiments with Lucifer yellow and neurobiotin confirmed that junctional communication patterns were altered in ways consistent with changes in connexin expression. The data thus suggest that intercellular communication is intimately involved in regulating epidermal wound repair.
The role of non-Saccharomyces yeasts in industrial winemaking. (1998)

The fermentation of grape juice into wine is a complex microbiological process, in which yeasts play a central role. Traditionally, identification and characterization of yeast species have been based on morphological and physiological characteristics. However, the application of molecular biology techniques represents an alternative to the traditional methods of yeast identification and are becoming an important tool in solving industrial problems. Although Saccharomyces cerevisiae is responsible for the alcoholic fermentation, the presence of non-Saccharomyces species could be important since they produce secondary metabolites, which can contribute to the final taste and flavor of wines.
Determination of Serum Unbound Bilirubin for Prediction of Kernicterus in Low Birthweight infants (1992)

Serum unbound bilirubin concentrations (UBC) and serum total bilirubin concentrations (TBC) were measured serially in 138 low birthweight (LBW) infants treated with phototherapy for non-hemolytic hyperbilirubinemia. We attempted to assign the suitable critical UBC levels for predicting bilirubin encephalopathy into two different birthweight groups: a very low birthweight (VLBW) group (birthweight < 1,500 g) and an LBW group (birthweight between 1,500 g and 2,499 g). Twelve infants were diagnosed as 'at risk' for kernicterus, of whom 11 had signs of acute bilirubin encephalopathy and received exchange transfusion. One VLBW infant had neurological sequelae at a 3 year follow-up, although exchange transfusion was not carried out because of low TBC. Sensitivity and specificity for predicting kernicterus were calculated at different UBC levels between 0.6 microgram/dl and 1.5 micrograms/dl and TBC levels between 8 mg/dl and 26 mg/dl. The receiver-operating characteristic (ROC) curves plotted for UBC as a predictor of kernicterus were clearly shifted up and to the left compared with the curves for TBC in the VLBW and LBW groups. Thus, the UBC measurement may well provide a more rational basis for evaluating the risk of kernicterus in LBW infants. The optimal cut-off points were derived from these curves. In the VLBW group, the sensitivity was 100% and the specificity was 96% for a UBC of 0.8 microgram/dl, and 80% and 64% for a TBC of 11 mg/dl. In the LBW group, the sensitivity was 100% and the specificity was 98% for a UBC of 1.0 microgram/dl and 71% and 78% for a TBC of 16 mg/dl.(ABSTRACT TRUNCATED AT 250 WORDS)
Heyman Wants To Own All Of ISP (2002)

RETURN TO ISSUEPREVNewsNEXTHeyman Wants To Own All Of ISPMARC REISCHCite this: Chem. Eng. News 2002, 80, 28, 11Publication Date (Print):July 15, 2002Publication History Published online13 November 2010Published inissue 15 July 2002https://pubs.acs.org/doi/10.1021/cen-v080n028.p011ahttps://doi.org/10.1021/cen-v080n028.p011anewsACS PublicationsCopyright © 2002 AMERICAN CHEMICAL SOCIETYArticle Views8Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Inorganic carbon compounds Get e-Alerts
Expression of the S-100 proteins MRP-8 and -14 in ischemic brain lesions (1997)

So far, microglial activation in cerebral ischemia has only been studied in different animal models. We have investigated the activation of microglial cells in human cerebral ischemia. As a marker for the activation of these "brain macrophages," we have used the macrophage inhibitor factor-related-proteins MRP-8 and MRP-14, which belong to the calcium binding S-100 protein family. The proteins can be detected on microglial cells in bacterial encephalitis and Alzheimer's disease but have so far not been studied in non-inflammatory diseases, in which microglial activation also occurs. Antibodies against MRP-8 and -14 detected ramified microglial cells within the first 3 days after cerebral infarction. Labeled cells were found selectively in the periinfarctional area. To support the notion that these cells belong to the locally activated resident microglial population, we studied their proliferation rate by staining the Ki-67 antigen with the antibody MIB-1. Double-labeling clearly showed that in the early phase of cerebral infarction microglial cells in the periinfarctional area express MRP-8 and -14 and also proliferate. Surprisingly, MRPs are expressed no longer than 3 days post infarction. This indicates that the activation of the resident microglia is an early step of tissue reaction after cerebral infarction. Additionally, we found evidence that microglial cells contribute to the population of phagocytes only during the first 3 days post infarction. The majority of lipid phagocytes found in the later stages are obviously recruited from the blood-borne macrophage pool.
Lac repressor purification without inactivation of DNA binding activity (1977)

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 +/- 10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.
Detection of the Lyme disease bacterium, Borrelia burgdorferi, by using the polymerase chain reaction and a nonradioisotopic gene probe (1991)

A 419-bp region of the flagellin gene sequence of Borrelia burgdorferi was used as a target for the polymerase chain reaction. With a nonradioactively labeled gene-specific probe, sensitivity to as few as 1 to 10 spirochetes was observed. The targeted gene fragment was conserved in the American and European strains of B. burgdorferi tested and among several other pathogenic borreliae.
Adaptive support-weight approach for correspondence search (2006)

We present a new window-based method for correspondence search using varying support-weights. We adjust the support-weights of the pixels in a given support window based on color similarity and geometric proximity to reduce the image ambiguity. Our method outperforms other local methods on standard stereo benchmarks.
Dominant-negative Effects of COL7A1 Mutations Can be Rescued by Controlled Overexpression of Normal Collagen VII (2009)

Dominant-negative interference by glycine substitution mutations in the COL7A1 gene causes dominant dystrophic epidermolysis bullosa (DDEB), a skin fragility disorder with mechanically induced blistering. Although qualitative and quantitative alterations of the COL7A1 gene product, collagen VII, underlie DDEB, the lack of direct correlation between mutations and the clinical phenotype has rendered DDEB less amenable to therapeutic targeting. To delineate the molecular mechanisms of DDEB, we used recombinant expression of wild-type (WT) and mutant collagen VII, which contained a naturally occurring COL7A1 mutation, G1776R, G2006D, or G2015E, for characterization of the triple helical molecules. The mutants were co-expressed with WT in equal amounts and could form heterotrimeric hybrid triple helices, as demonstrated by affinity purification and mass spectrometry. The thermal stability of the mutant molecules was strongly decreased, as evident in their sensitivity to trypsin digestion. The helix-to-coil transition, T(m), of the mutant molecules was 31-34 degrees C, and of WT collagen VII 41 degrees C. Co-expression of WT with G1776R- or G2006D-collagen VII resulted in partial intracellular retention of the collagen, and mutant collagen VII had reduced ability to support cell adhesion. Intriguingly, controlled overexpression of WT collagen VII gradually improved the thermal stability of the collective of collagen VII molecules. Co-expression in a ratio of 90% WT:10% mutant increased the T(m) to 41 degrees C for G1776R-collagen VII and to 39 degrees C for G2006D- and G2015E-collagen VII. Therefore, increasing the expression of WT collagen VII in the skin of patients with DDEB can be considered a valid therapeutic approach.
Designing effective auctions for treasury securities (1997)

Most discussions of treasury auction design focus on the choice between two methods for issuing securities—uniform-price or discriminatory auctions. Although auction theory and much recent research appear to favor the uniform-price method, most countries conduct their treasury auctions using the discriminatory format. What are the main issues underlying the debate over effective auction design? The widespread growth of public debt in industrial countries in the 1980s has intensif ied policymakers’ concern with implementing effective methods to sell government securities. Treasury auctions—in which a government sells securities to finance its debt—are a natural, but often overlooked, candidate for institutional reform. By broadening participation in treasury auctions and increasing auction revenues, governments could potentially save millions of dollars. Consider, for example, the savings that might be realized by a country auctioning $1 trillion of securities annually. (In recent years, the U.S. Treasury has auctioned more than $2 trillion of marketable securities annually.) If a more cost-effective design could be identified and adopted, each 0.01 percent reduction in auction yields achieved through the design change would lower that country’s annual federal deficit by more than $100 million. In this edition of Current Issues, we examine the two main auction methods in use today to issue treasury securities—discriminatory and uniform-price auctions. Drawing on the theory of auction design and the empirical findings of earlier researchers, we discuss the revenue potential of these two auction methods and their vulnera-bility to noncompetitive behavior by bidders. We then compare researchers ’ views of the two methods with the actual practices of forty-two countries holding auctions of treasury securities. Surprisingly, while much of the lit-erature suggests that uniform-price auctions may out-perform discriminatory auctions in producing revenues for treasuries and limiting the scope for noncompetitive behavior, most countries conduct their treasury auctions using the more traditional discriminatory format. This split between theory and practice is likely to lead to increased experimentation with new auction formats in the next several years.
Ruthenium‐Catalyzed Ring‐Closing Metathesis: Recent Advances, Limitations and Opportunities (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Optical Fibre Pressure Sensors in Medical Applications (2015)

This article is focused on reviewing the current state-of-the-art of optical fibre pressure sensors for medical applications. Optical fibres have inherent advantages due to their small size, immunity to electromagnetic interferences and their suitability for remote monitoring and multiplexing. The small dimensions of optical fibre-based pressure sensors, together with being lightweight and flexible, mean that they are minimally invasive for many medical applications and, thus, particularly suited to in vivo measurement. This means that the sensor can be placed directly inside a patient, e.g., for urodynamic and cardiovascular assessment. This paper presents an overview of the recent developments in optical fibre-based pressure measurements with particular reference to these application areas.
<title>Irrotationality of uniform electro-osmosis</title> (1999)

Steady electroosmotic flow of uniform liquids in uniform media is irrotational provided the electric double layers adjacent to surfaces are negligibly thin, the surfaces are non-conducting and impermeable, and the total pressure imposed at inlets and outlets is uniform. Because many microfluidic devices employing electroosmosis approximately satisfy these requirements, this ideal electrosmosis is a limiting case with considerable practical significance. In ideal electroosmosis, fluid motion follows current lines. Flow-fields have no Reynolds number dependence and are everywhere proportional to the electric field. Both fields may be obtained by a single solution of the Laplace equation. In this paper, we discuss these features of ideal electroosmotic flows and present particle-image derived velocity fields that confirm ideal flow conditions in glass microchannel networks.
Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells. (1987)

We studied the ability of mammalian cells to repair single-stranded nicks, gaps, and loops in DNA duplexes. Heteroduplexes prepared from derivatives of the shuttle vector pSV2neo were introduced into monkey COS cells. After replication, the plasmids were recovered and used to transform Escherichia coli. Plasmid DNA from the recovered colonies was tested for repair at each of six different sites. We observed that mammalian cells are capable of repairing single-stranded gaps and free single-stranded ends most efficiently. Regions containing twin loops were recognized, and one of the loops was excised. Portions of the molecules containing small single loops were also repaired. Markers which were 58 nucleotides apart were corepaired with nearly 100% efficiency, while markers which were 1,000 nucleotides or more apart were never corepaired. The mechanisms involved in heteroduplex repair in mammalian cells seem to be similar to those involved in repairing DNA lesions caused by physical and chemical agents.