gebhart/patrepeval huggingface dataset:
Visualization of maltose uptake in living yeast cells by fluorescent nanosensors (2002)

Compartmentation of metabolic reactions and thus transport within and between cells can be understood only if we know subcellular distribution based on nondestructive dynamic monitoring. Currently, methods are not available for in vivo metabolite imaging at cellular or subcellular levels. Limited information derives from methods requiring fixation or fractionation of tissue (1, 2). We thus developed a flexible strategy for designing protein-based nanosensors for a wide spectrum of solutes, allowing analysis of changes in solute concentration in living cells. We made use of bacterial periplasmic binding proteins (PBPs), where we show that, on binding of the substrate, PBPs transform their hinge-bend movement into increased fluorescence resonance energy transfer (FRET) between two coupled green fluorescent proteins. By using the maltose-binding protein as a prototype, nanosensors were constructed allowing in vitro determination of FRET changes in a concentration-dependent fashion. For physiological applications, mutants with different binding affinities were generated, allowing dynamic in vivo imaging of the increase in cytosolic maltose concentration in single yeast cells. Control sensors allow the exclusion of the effect from other cellular or environmental parameters on ratio imaging. Thus the myriad of PBPs recognizing a wide spectrum of different substrates is suitable for FRET-based in vivo detection, providing numerous scientific, medical, and environmental applications.
Characterization of a Family of Algorithms for Generalized Discriminant Analysis on Undersampled Problems (2005)

A generalized discriminant analysis based on a new optimization criterion is presented. The criterion extends the optimization criteria of the classical Linear Discriminant Analysis (LDA) when the scatter matrices are singular. An efficient algorithm for the new optimization problem is presented. The solutions to the proposed criterion form a family of algorithms for generalized LDA, which can be characterized in a closed form. We study two specific algorithms, namely Uncorrelated LDA (ULDA) and Orthogonal LDA (OLDA). ULDA was previously proposed for feature extraction and dimension reduction, whereas OLDA is a novel algorithm proposed in this paper. The features in the reduced space of ULDA are uncorrelated, while the discriminant vectors of OLDA are orthogonal to each other. We have conducted a comparative study on a variety of real-world data sets to evaluate ULDA and OLDA in terms of classification accuracy.
A sketch recognition interface that recognizes hundreds of shapes in course-of-action diagrams (2010)

Sketch recognition is the automated recognition of hand drawn diagrams. Military course-of-action (COA) diagrams are used to depict battle scenarios. The domain of military course of action diagrams is particularly interesting because it includes tens of thousands of different geometric shapes, complete with many additional textual and designator modifiers. Existing sketch recognition systems recognize on the order of at most 20 different shapes. Our sketch recognition interface recognizes 485 different freely drawn military course-of-action diagram symbols in real time, with each shape containing its own elaborate set of text labels and other variations. We are able to do this by combining multiple recognition techniques in a single system. When the variations (not allowable by other systems) are factored in, our system is several orders of magnitude larger than the next biggest system. On 5,900 hand-drawn symbols drawn by 8 researchers, the system achieves an accuracy of 90% when considering the top 3 interpretations and requiring every aspect of the shape (variations, text, symbol, location, orientation) to be correct.
Grounding spatial relations for human-robot interaction (2013)

We propose a system for human-robot interaction that learns both models for spatial prepositions and for object recognition. Our system grounds the meaning of an input sentence in terms of visual percepts coming from the robot's sensors in order to send an appropriate command to the PR2 or respond to spatial queries. To perform this grounding, the system recognizes the objects in the scene, determines which spatial relations hold between those objects, and semantically parses the input sentence. The proposed system uses the visual and spatial information in conjunction with the semantic parse to interpret statements that refer to objects (nouns), their spatial relationships (prepositions), and to execute commands (actions). The semantic parse is inherently compositional, allowing the robot to understand complex commands that refer to multiple objects and relations such as: “Move the cup close to the robot to the area in front of the plate and behind the tea box”. Our system correctly parses 94% of the 210 online test sentences, correctly interprets 91% of the correctly parsed sentences, and correctly executes 89% of the correctly interpreted sentences.
Plasmapheresis in multiple sclerosis (1980)

In six of eight patients with chronic progressive multiple sclerosis (MS) who were previously unresponsive to corticosteroid medication, moderate but unequivocal improvement occurred after short-term plasmapheresis, prednisone, and azathioprine therapy. In four patients, improvement gradually disappeared in the 2 to 3 months after therapy, and two patients have remained improved. There were no exacerbations during the treatment period. No consistent improvement in evoked response batteries was seen after plasmapheresis; IgG levels in cerebrospinal fluid were lowered by plasmapheresis. A controlled trial to determine the efficacy of plasmapheresis in the treatment of MS is warranted.
Radio propagation measurements at 850 MHz, 1.7 GHz and 4 GHz inside two dissimilar office buildings (1990)

Time delay spread and signal level measurements were made in two dissimilar office buildings at 850 MHz, 1.7 GHz and 4.0 GHz. The RMS time delay spread did not deteriorate with increasing frequency in these buildings. Its maximum value at the three frequencies did not exceed 270 ns at the larger building and 100 ns at the smaller building. Attenuation was nearly the same at the three frequencies, but decreased slightly with increasing frequency, within the limits of the experiment&apos;s accuracy.
UltraPulse Carbon Dioxide Laser with CPG Scanner for Full-Face Resurfacing for Rhytids, Photoaging, and Acne Scars (1997)

Eleven female patients are reported who underwent full-face resurfacing. Three patients were treated for cosmetic rhytids, five for residual acne scarring, and three for photoaging. There were no complications or side effects in this group of patients. Reepithelialization was achieved in an average of 9.3 days, and erythema disappeared in an average of 8.9 weeks. The UltraPulse carbon dioxide laser with computerized pattern generator (CPG) scanner allows a rapid, uniform laserbrasion. The sequence of the procedure involves close application of adjacent squares at 60 W, 200 to 300 ml, at moderate density. Skin preparation with Retin-A and bleaching agents is important for best wound healing. Postoperative wound care includes maintenance of a moist environment and Zovirax for herpes prophylaxis.
High-throughput particle separation and concentration using spiral inertial filtration (2014)

A spiral inertial filtration (SIFT) device that is capable of high-throughput (1 ml/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude is reported. This device is able to remove large fractions of sample fluid from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the SIFT microsystem for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were then achieved by maintaining either the average fluid velocity or the Dean number throughout the entire length of the channel during the incremental removal of sample fluid. The SIFT device was able to separate MCF7 cells spiked into whole blood from the non-target white blood cells (WBC) with a recovery of nearly 100% while removing 93% of the sample volume, which resulted in a concentration enhancement of the MCF7 cancer cells by a factor of 14.
ENIGMA BEFORE ULTRA POLISH WORK AND THE FRENCH CONTRIBUTION (1987)

(1987). ENIGMA BEFORE ULTRA POLISH WORK AND THE FRENCH CONTRIBUTION. Cryptologia: Vol. 11, No. 3, pp. 142-155.
Small intestinal CD8+TCRγδ+NKG2A+ intraepithelial lymphocytes have attributes of regulatory cells in patients with celiac disease (2008)

Intraepithelial lymphocytes (IELs) bearing the gammadelta TCR are more abundant in the small intestinal mucosa of patients with celiac disease (CD) compared with healthy individuals. However, their role in disease pathogenesis is not well understood. Here, we investigated the functional attributes of TCRgammadelta+ IELs isolated from intestinal biopsies of patients with either active celiac disease (ACD) or those on a gluten-free diet (GFD). We found that compared with individuals with ACD, individuals on GFD have a higher frequency of CD8+TCRgammadelta+ IELs that express the inhibitory NK receptor NKG2A and intracellular TGF-beta1. TCR triggering as well as cross-linking of NKG2A increased both TGF-beta1 intracellular expression and secretion in vitro. Coculture of sorted TCRgammadelta+NKG2A+ IELs, IL-15-stimulated TCRalphabeta+ IELs, and HLA-E+ enterocytes resulted in a decreased percentage of cytotoxic CD8+TCRalphabeta+ IELs expressing intracellular IFN-gamma and granzyme-B and surface NKG2D. This inhibition was partially abrogated by blocking either TGF-beta alone or both NKG2A and HLA-E. Thus, our data indicate that suppression was at least partially mediated by TGF-beta secretion as a result of engagement of NKG2A with its ligand, HLA-E, on enterocytes and/or TCRalphabeta+ IELs. These findings demonstrate that human small intestinal CD8+TCRgammadelta+ IELs may have regulatory potential in celiac disease.
Viral vectors for gene delivery and gene therapy within the endocrine system (2000)

The transfer of genetic material into endocrine cells and tissues, both in vitro and in vivo, has been identified as critical for the study of endocrine mechanisms and the future treatment of endocrine disorders. Classical methods of gene transfer, such as transfection, are inefficient and limited mainly to delivery into actively proliferating cells in vitro. The development of viral vector gene delivery systems is beginning to circumvent these initial setbacks. Several kinds of viruses, including retrovirus, adenovirus, adeno-associated virus, and herpes simplex virus, have been manipulated for use in gene transfer and gene therapy applications. As different viral vector systems have their own unique advantages and disadvantages, they each have applications for which they are best suited. This review will discuss viral vector systems that have been used for gene transfer into the endocrine system, and recent developments in viral vector technology that may improve their use for endocrine applications - chimeric vectors, viral vector targeting and transcriptional regulation of transgene expression.
Serum-stimulated cell growth causes oscillations in casein kinase II activity (1989)

We have tested the effects of serum-stimulated growth of quiescent WI38 human lung fibroblasts on cellular casein kinase II (CK-II) activity. Using the casein kinase II synthetic substrate RRREEETEEE we find a transient 6-fold elevation in CK-II activity in cell homogenates within 30 min following serum stimulation. Additional cycles of CK-II activation and inactivation are seen at 12 and 24 h after stimulation. The oscillations in CK-II activity are largely independent of de novo protein synthesis, and, thus, are likely to reflect cycles of post-translational activation and inhibition of the cellular kinase pool. In contrast to the activity profile of CK-II, we find that cyclic AMP-dependent protein kinase is rapidly inhibited upon serum-stimulation of WI38 cells. These results demonstrate that CK-II activity is subject to unique cellular regulation during proliferation and are consistent with the postulate that CK-II plays an important role in cell growth.
A 0.13 <formula formulatype="inline"><tex Notation="TeX">$\mu$</tex> </formula><emphasis emphasistype="roman">m</emphasis> CMOS Quad-Band GSM/GPRS/EDGE RF Transceiver Using a Low-Noise Fractional-N Frequency Synthesizer and Direct-Conversion Architecture (2009)

This paper presents a single-chip CMOS quad-band (850/900/1800/1900 MHz) RF transceiver for GSM/GPRS/EDGE applications which adopts a direct-conversion receiver, a direct-conversion transmitter and a fractional-N frequency synthesizer with a built-in DCXO. In the GSM mode, the transmitter delivers 4 dBm of output power with 1deg RMS phase error and the measured phase noise is -164.5 dBc/Hz at 20 MHz offset from a 914.8 MHz carrier. In the EDGE mode, the TX RMS EVM is 2.4% with a 0.5 dB gain step for the overall 36 dB dynamic range. The RX NF and IIP3 are 2.7 dB/-12 dBm for the low bands (850/900 MHz) and 3 dB/-11 dBm for the high bands (1800/1900 MHz). This transceiver is implemented in 0.13 mum CMOS technology and occupies 10.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The device consumes 118 mA and 84 mA in TX and RX modes from 2.8 V, respectively and is housed in a 5 times 5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> 40-pin QFN package.
De-indirection for flash-based SSDs with nameless writes (2012)

We present Nameless Writes, a new device interface that removes the need for indirection in modern solid-state storage devices (SSDs). Nameless writes allow the device to choose the location of a write; only then is the client informed of the name (i.e., address) where the block now resides. Doing so allows the device to control block-allocation decisions, thus enabling it to execute critical tasks such as garbage collection and wear leveling, while removing the need for large and costly indirection tables. We demonstrate the effectiveness of nameless writes by porting the Linux ext3 file system to use an emulated nameless-writing device and show that doing so both reduces space and time overheads, thus making for simpler, less costly, and higher-performance SSD-based storage.
TRANSARTERIAL ELECTROCOAGULATION THERAPY OF A PSEUDOANEURYSM IN THE HEAD OF THE PANCREAS (1975)

Because chemical and transcatheter embolizations for control of bleeding were inappropriate, electrocoagulation through a guidewire was used for arterial occlusion. The power was alternating current. The effectiveness of the procedure was confirmed by arteriograms done immediately and 5 months later.
马克思《资本论》第2卷的形成过程 (1986)

研究马克思的经济学遗产,迫切要求在研究这一遗产的产生和发展过程中以完整的观点对待这一遗产.对作为《资本论》有机组成部分的《资本论》第2卷的形成过程进行研究,能够更好地理解它的内容及其在马克思经济学遗产中的地位,并了解它对社会主义政治经济学的意义.列宁指出:马克思所作出的另一个极其重要的新贡献,就是他在《资本论》第2卷中对社会总资本的再生产的分析.1857—1858年手稿《资本论》第1卷的来源是1857一1858年的手稿,这是《资本论》的初稿.其中已经包括了属于未来第2卷的相当广泛的材料.在这个手稿中,马克思对再生产理论的研究
Novel type I interferon IL-28A suppresses hepatitis C viral RNA replication (2005)

Interferon alpha (IFN-alpha)-based therapy is the currently approved treatment for chronic hepatitis C viral infection. The sustained antiviral response rate is approximately 50% for genotype-1 infection. The major challenge to the HCV community is to improve antiviral efficacy and to reduce the side effects typically seen in IFNalpha-based therapy. One of the strategies is to identify new interferons, which may have better efficacy and less undesirable side effects. In this report, we examined the role of IL-28A (IFN lambda2), a novel type I IFN, in suppression of human hepatitis C viral RNA replication. We have cloned both the human genomic DNA and cDNA of IL-28A, and evaluated their biological activity using HCV RNA replicon cell culture system. The results show that IL-28A effectively inhibits HCV subgenomic RNA replication in a dose-dependent manner. Treatment of human hepatoma cells with IL-28A activates the JAK-STAT signaling pathway and induces the expression of some interferon-stimulated genes (ISGs), such as 6-16 and 1-8U. We also demonstrate that IL-28A induces expression of HLA class I antigens in human hepatoma cells. Moreover, IL-28A appears to specifically suppress HCV IRES-mediated translation. Although IL-28A receptor shares one subunit with the IL-10 receptor, IL-10 treatment has no detectable effect on IL-28A-induced antiviral activity. Interestingly, IL-28A can synergistically enhance IFNalpha antiviral efficacy. Our results suggest that IL-28A antiviral activity is associated with the activation of the JAK-STAT signaling pathway and expression of ISGs. The effectiveness of IL-28A antiviral activity and its synergistic effect on IFN-alpha indicate that IL-28A may be potentially used to treat HCV chronic infection.
Human iPSC-Derived Miniature Organs: A Tool for Drug Studies (2014)

In vitro organogenesis is now becoming a realistic goal of stem cell biology, as one can obtain an unlimited number of pluripotent stem cells through reprogramming technology. One practical challenge is to develop a four-dimensional (4-D) stem cell culture system whereby multiple progenitors communicate in a spatiotemporal manner, as observed during in vivo organogenesis. This perspective summarizes the potential for emerging culture platforms in the future application of induced pluripotent stem cell (iPSC)-derived miniature organs by recapitulating early embryogenesis.
Triple-negative breast carcinomas are a heterogeneous entity that differs between young and old patients (2010)

These results show that there were significant molecular differences between the triple-negative basal-like breast carcinomas that were diagnosed in younger women and those that were diagnosed in older women. These findings may provide a basis for describing the more aggressive phenotype of the triple-negative breast carcinomas observed in younger women.
Enzyme Defect Associated with a Sex-Linked Human Neurological Disorder and Excessive Purine Synthesis (1967)

A sex-linked familial neurological disease consisting of cerebral palsy, mental retardation, choreoathetosis, and compulsive aggressive behavior is associated with a loss of an enzyme that participates in purine metabolism, namely, hypoxanthine-guanine phosphoribosyltransferase. The production of excessive uric acid in this disorder implies that the enzyme is involved in the normal regulation of purine biosynthesis. This is the first example of a relation between a specific enzyme defect and abnormal compulsive behavior. It is also the first enzyme defect in purine metabolism demonstrated in a neurological disease.
Dipeptidyl Peptidase-4 (DPP-4) Inhibitors In the Management of Diabetes. (2010)

Secretion of GLP-1 occurs from the enteroendocrine L cells of the distal small intestine, whereas GIP is mainly secreted from the K cells in the proximal small intestine. 4 In the early 1900s, research involving the treatment of glycosuria with the administration of intestinal extracts first supported the incretin (INtestinal seCRETion of INsulin) effect that is mediated by these hormones. 5 The incretin effect refers to the greater amount of insulin secretion noted with giving oral glucose versus a comparable intravenous (IV) dosage, suggesting that oral ingestion stimulates pancreatic beta cells and the regulation of glucose. 2 This effect is reduced in patients with type-2 diabetes and is thought to be related to decreased insulinotropic action of GIP but not GLP-1. 4 In addition, continuous infusions of GLP-1 increase insulin secretion in patients with type-2 diabetes; however, higher doses of GIP have not been shown to do the same. 2
A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography (2000)

We are developing assays for noninvasive, quantitative imaging of reporter genes with positron emission tomography (PET), for application both in animal models and in human gene therapy. We report here a method to improve the detection of lower levels of PET reporter gene expression by utilizing a mutant herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) as a PET reporter gene. The HSV1-sr39tk mutant was identified from a library of site-directed mutants. Accumulation (net uptake) of the radioactively labeled substrates [8-(3)H]penciclovir ([8-(3)H]PCV), and 8-[(18)F]fluoropenciclovir (FPCV) in C6 rat glioma cells expressing HSV1-sr39tk is increased by a factor of approximately 2.0 when compared with C6 cells expressing wild-type HSV1-tk. The increased imaging sensitivity of HSV1-sr39tk when FPCV is used is also demonstrated in vivo both with tumor cells stably transfected with either HSV1-tk or HSV1-sr39tk, and after hepatic delivery of HSV1-tk or HSV1-sr39tk by using adenoviral vectors. The use of HSV1-sr39tk as a PET reporter gene and FPCV as a PET reporter probe results in significantly enhanced sensitivity for imaging reporter gene expression in vivo.
Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element (2002)

A systematic analysis was carried out to examine the effects of ribonucleotide substitution at various locations within the promoter element for T7 RNA polymerase. Ribonucleotides could be introduced at most positions without significantly decreasing transcription efficiency. A critical window of residues that were intolerant of RNA substitution was defined for both the nontemplate and template strands of the promoter. These residues are involved in important contacts with the AT-rich recognition loop, specificity loop, and beta-intercalating hairpin of the polymerase. These results highlight the malleability of T7 RNA polymerase in recognizing its promoter element and suggest that promoters with altered backbone conformations may be used in molecular biology applications that use T7 RNA polymerase for in vitro transcription.
Isolation of novel human endogenous retrovirus-like elements with foamy virus-related pol sequence (1995)

A new class of reverse transcriptase coding sequences was detected in reverse-transcribed RNAs from human placenta by polymerase chain amplification with primers in highly conserved regions of the pol gene of mammalian retroviruses and retrotransposons. Using one of these novel sequences as a probe to screen a human genomic library, we isolated retrovirus-like elements bordered by long terminal repeats and having a potential leucine tRNA primer-binding site. Determination of the complete nucleotide sequence (6,591 bp) of one of these elements, termed HERV-L (for human endogenous retrovirus with leucine tRNA primer), revealed domains of amino acid similarities to retroviral reverse transcriptase and integrase proteins. In addition, a region with homologies to dUTPase proteins was found unexpectedly downstream from the integrase domain. Amino acid sequence and phylogenetic analysis indicate that the HERV-L pol gene is related to that of foamy retroviruses. HERV-L-related sequences are detected in several mammalian species and have expanded in primate and mouse genomes up to 100 to 200 copies.