gebhart/patrepeval huggingface dataset:
Computer‐Aided Molecular Design of Hydrogen Bond Equivalents of Nucleobases: Theoretical Study of Substituent Effects on the Hydrogen Bond Energies of Nucleobase Pairs (2003)

Abstract Substituent effects on the hydrogen bond energies of Watson−Crick‐type base pairs, formed between a chemically modified nucleic acid base derivative and an unmodified one, were evaluated by ab initio molecular orbital theory. Different trends were observed in the relationship between the substituent and the hydrogen bond energy in each base pair. The predicted hydrogen bond energies correlated well with the experimentally measured binding properties, and so ab initio calculation appears to be an effective method with which to estimate the stabilities of base pairs between chemically modified nucleic acid bases. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2003)
Short chain fatty acid-induced colitis in mice. (1989)

Short-chain fatty acids (e.g. acetic, propionic and butyric acid) are metabolic products produced by colonic bacteria. Since intra-rectal instillation of dilute acetic acid to rats and cats has been reported to produce a diffuse colitis similar to the human disease, in our study we have investigated the effect of intra-rectal butyric acid in mice. Male BKA mice (n = 6) received 01 ml of dilute butyric acid by intra-rectal instillation which was flushed out with saline after 10 sec. Control animals received saline alone. The severity of observed inflammatory responses was assessed using an arbitrary scoring system based on clinical, macroscopic and histological features of colitis. Colitic oedema was measured as percentage (%) tissue water compared with that in saline controls. The peak oedema response was found to be at around 4 hours. A concentration-dependent symptomatic response was obtained at concentrations between 1% and 12% of butyric acid. Oedema production was similar at all concentrations above 1%. The 3% butyric acid response produced a moderate colitis with mild erythema, oedema, crypt abscess formation, goblet cell depletion and cellular infiltration without total loss of mucosal architecture. The colitogenic action of butyric acid in mouse colon could not be reproduced by low pH alone, nor by the butyrate anion at neutral or alkaline pH. These results indicate that intra-rectal butyric acid induces a consistent and reproducible colitis in mice.
Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12 (1981)

We report here on the cloning of a gene located within the ompB locus of E. coli K-12. This gene, designated ompR, resides on a 10.9-kilobase EcoRI fragment that we cloned, using a lambda vector and in vitro packaging techniques. By subcloning portions of this fragment into the high-copy-number plasmid pBR322, we have isolated the ompR gene on a 1.4-kilobase EcoRI-AvaI fragment. This fragment has been characterized physically and will facilitate a more detailed study of the role and mechanism of porin regulation by the ompB locus.
Prognostic significance of monocarboxylate transporter 4 expression in patients with colorectal cancer (2011)

Cancer cells generally have a high rate of glycolysis and produce larger quantities of lactate as compared to the surrounding normal cells. Monocarboxylate transporter 4 (MCT4) is one of the proton pumps exchanging the lactate through the plasma membrane. The prognostic significance of MCT4 expression has not been evaluated in patients with colorectal cancer (CRC). Surgical specimens from 105 CRC patients were immunohistochemically stained using a polyclonal anti-MCT4 antibody. The relationships among the MCT4 expression, clinicopathological factors and prognosis were evaluated. A total of 53 (50.5%) of the 105 patients with CRC were determined to have tumors positive for MCT4 expression. The expression of MCT4 significantly correlated with the tumor size, depth of invasion, lymph node metastasis, distant metastasis and TNM staging. The survival rate of the patients who were positive for MCT4 expression was significantly lower than that of patients with negative MCT4 expression. Positive MCT4 expression was a significantly poor prognostic factor, as determined by both univariate and multivariate analyses. Therefore, positive MCT4 expression appears to be a useful marker for tumor progression and prognosis in patients with CRC.
The GLC7 Type 1 Protein Phosphatase Is Required for Glucose Repression in Saccharomyces cerevisiae (1994)

We cloned the GLC7/DIS2S1 gene by complementation of the cid1-226 mutation, which relieves glucose repression in Saccharomyces cerevisiae. GLC7 encodes the catalytic subunit of type 1 protein phosphatase (PP1). Genetic analysis and sequencing showed that cid1-226 is an allele of GLC7, now designated glc7-T152K, which alters threonine 152 to lysine. We also show that the glc7-1 and glc7-T152K alleles cause distinct phenotypes: glc7-1 causes a severe defect in glycogen accumulation but does not relieve glucose repression, whereas glc7-T152K does not prevent glycogen accumulation. These findings are discussed in light of evidence that interaction with different regulatory or targeting subunits directs the participation of PP1 in diverse cellular regulatory mechanisms. Finally, genetic studies suggest that PP1 functions antagonistically to the SNF1 protein kinase in the regulatory response to glucose.
Total Synthesis of (−)-Epothilone A (1999)

Epothilones 1 (1 A: R = H; 1 B: R = Me) as promising, new microtubule-stabilizing natural products with taxol-like biological activity were isolated from myxo bacteria. A flexible and convergent total synthesis of epothilone A (1 A) and two formal total syntheses of epothilone B (1 B) for an easy and facile access to epothilones are reported.
CRISPR‐based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii (2016)

Solventogenic clostridia are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools would facilitate physiological studies aimed both at improving our understanding of metabolism and optimizing solvent productivity through metabolic engineering. Here we have developed an all-in-one, CRISPR-based genome editing plasmid, pNICKclos, that can be used to achieve successive rounds of gene editing in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB 8052 with efficiencies varying from 6.7% to 100% and 18.8% to 100%, respectively. The plasmid specifies the requisite target-specific guide RNA, the gene encoding the Streptococcus pyogenes Cas9 nickase and the genome editing template encompassing the gene-specific homology arms. It can be used to create single target mutants within three days, with a further two days required for the curing of the pNICKclos plasmid ready for a second round of mutagenesis. A S. pyogenes dCas9-mediated gene regulation control system, pdCASclos, was also developed and used in a CRISPRi strategy to successfully repress the expression of spo0A in C. acetobutylicum and C. beijerinckii. The combined application of the established high efficiency CRISPR-Cas9 based genome editing and regulation control systems will greatly accelerate future progress in the understanding and manipulation of metabolism in solventogenic clostridia.
High level desmin expression depends on a muscle-specific enhancer. (1991)

Expression of the desmin gene is increased several-fold during myogenesis. To identify the DNA sequences involved in this regulation, a 16-kilobase fragment containing a 4.5-kilobase 5'-flanking region and the complete sequence of the human desmin gene has been isolated. The transcription initiation sites were determined by S1 nuclease mapping and primer extension analysis. A series of constructs have been made by fusing different lengths of human desmin 5'-flanking fragments to the bacterial chloramphenicol acetyl transferase (CAT) gene, these constructs were introduced either into mouse myogenic cells or into nonmyogenic cells. Comparison of the transient expression of CAT between myogenic and nonmyogenic cells demonstrated that the sequence including 228 base pairs upstream of the transcription initiation site is sufficient to confer low level, muscle-specific expression of the desmin gene. A negative region was located between -693 and -228 base pairs relative to the desmin mRNA start site. High level expression of the gene depends on a 280-base pair muscle-specific enhancer located between -693 and -973 base pairs upstream of the transcription initiation site. This enhancer can activate either the desmin promoter or heterologous promoters about 20-70-fold in myogenic cells. The enhancer, which also acts in myoblast, is discussed in the context of early expression of the desmin during skeletal myogenesis in the mammalian embryo.
Study of Silicon Oxycarbide(SiOC) as Anode Materials for Li-ion Batteries (2013)

The principal object of this thesis is the investigation of silicon oxycarbide (SiOC) ceramics as anode material for Li-ion batteries. The investigated materials are prepared by cross linking commercial polymer siloxanes via hydrosylilation reactions or hybrid alkoxide precursors via sol-gel. The cross linked polymer networks are then converted in to ceramic materials by a pyrolysis process in controlled argon atmosphere at 800-1300 °C.
In details the influence of carbon content on lithium storage properties is addressed for SiOC with the same O/Si atomic ratio of about 1. Detailed structural characterization studies are performed using complementary techniques which aim correlating the electrochemical behavior with the microstructure of the SiOC anodes. Results suggest that SiOC anodes behave as a composite material consisting of a disordered silicon oxycarbide phase having a very high first insertion capacity of ca 1300 mAh g-1 and a free C phase. However, the charge irreversibly trapped into the amorphous silicon oxycarbide network is also high. In consequence the maximum reversible lithium storage capacity of 650 mAh g-1 is measured on high-C content SiOCs with the ratio between amorphous silicon oxycarbide and the free C phase of  1:1. The high carbon content SiOC shows also an excellent cycling stability and performance at high charging/discharging rate with the stable capacity at 2C rate being around 200 mAh g-1.
Increasing the pyrolysis temperature has an opposite effect on the low-C and high-C materials: for the latter one the reversible capacity decreases following a known trend while the former shows an increase of
xi
the reversible capacity which has never been observed before for similar materials.
The influence of pyrolysis atmosphere on lithium storage capacity is investigated as well. It is found that pyrolysis in Ar/H2 mixtures, compared to the treatment under pure Ar, results into a decrease of the concentration of C dangling bonds as revealed by electron spin resonance (ESR) measurements. The sample prepared under Ar/H2 mixture shows an excellent cycling stability with an increase in the specific capacity of about 150 mAh g-1 compared to its analogues pyrolysed in pure argon atmosphere.
In order to study the role of porosity towards the lithium storage properties, a comparison of dense and porous materials obtained using same starting precursors is made. Porous SiOC ceramics are prepared by HF etching of the SiOC ceramics. HF etching removes a part of the amorphous silica phase from SiOC nanostructure leaving a porous structure. Porous ceramics with surface areas up to 640 m2 g-1 is obtained. The electrochemical charging/discharging results indicate that the porosity can help to increase the lithium storage capacity and it also leads to an enhanced cycling stability.
This work demonstrates clearly that silicon oxycarbide (SiOC) ceramics present excellent electrochemical properties to be applied as a promising anode material for lithium storage applications.
TUMOUR NECROSIS FACTOR ALPHA AND ITS SOLUBLE RECEPTORS PARALLEL CLINICAL DISEASE AND AUTOIMMUNE ACTIVITY IN SYSTEMIC LUPUS ERYTHEMATOSUS (1995)

Cytokines are believed to play an important role in the pathogenesis of systemic lupus erythematosus (SLE). However, for tumour necrosis factor alpha (TNF-alpha) both beneficial and deleterious effects have been reported. To obtain information about the involvement of this cytokine in the pathophysiology of SLE, serum levels of TNF-alpha, the soluble forms of the 55 and 75 kDa tumour necrosis factor receptors (TNF-R55 and TNF-R75), and interleukin-6 (IL-6) were measured by ELISA in nine female patients over a period of 2 yr. Compared to healthy controls, levels of TNF-alpha (median 47 pg/ml, range < 15-222 pg/ml), TNF-R55 (median 1.9 ng/ml, range 0.8-10.8 ng/ml), TNF-R75 (median 4.7 ng/ml, range 1.5-15 ng/ml) and IL-6 (median 3.5 pg/ml, range < 3.5-52 pg/ml) were significantly elevated in SLE patients (P < 0.0001 vs controls in all cases). There were strong correlations between TNF-alpha and its soluble receptors (P < 0.0001). Moreover, TNF-alpha and both TNF-Rs strongly correlated with clinical and serological parameters of disease activity, such as the European Consensus Lupus Activity Measurement (ECLAM) score, anti-dsDNA antibodies, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and anaemia (P < 0.0001 for all comparisons). TNF-alpha and TNF-R75 also correlated with IL-6 (P < 0.0001). However, no correlation between IL-6 and ECLAM was found, and the correlation of IL-6 with anti-dsDNA was relatively weak; in contrast, IL-6 correlated strongly with CRP and ESR (P < 0.0001). Although these data do not allow us ultimately to discriminate between beneficial and deleterious effects of TNF-alpha, they nevertheless suggest a central role for the TNF system in the pathophysiology of SLE.
Clinical features, genetics and potential therapeutic approaches for Birt–Hogg–Dubé syndrome (2014)

In the decade since <i>FLCN</i> was identified as causative for BHD, we have gained a greater understanding of the clinical spectrum and genetics of this cancer syndrome. Recent studies have identified interactions between FLCN and a variety of signaling pathways and cellular processes, notably AKT-mTOR. Currently, surgical intervention is the only available therapy for BHD-associated renal tumors. Effective therapies will need to target primary pathways/processes deregulated in <i>FLCN</i>-deficient renal tumors and fibrofolliculomas.
Modeling of time and document aging for request prediction—one step further (2000)

Article Modeling of time and document aging for request prediction—one step further Share on Authors: Ernst-Georg Haffner Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, Germany Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, GermanyView Profile , Uwe Roth Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, Germany Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, GermanyView Profile , Thomas Engel Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, Germany Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, GermanyView Profile , Christoph Meinel Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, Germany Institute of Telematics, Bahnhofsstr. 30-32, 54292 Trier, GermanyView Profile Authors Info & Claims SAC '00: Proceedings of the 2000 ACM symposium on Applied computing - Volume 2March 2000 Pages 984–990https://doi.org/10.1145/338407.338714Online:01 March 2000Publication History 3citation138DownloadsMetricsTotal Citations3Total Downloads138Last 12 Months2Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Lusotopie, vol.13, n°1 (2006)

http://www.lusotopie.sciencespobordeaux.fr/somma2006.htm&lt;br /&gt;en ligne, en texte intégral sur la Base de données&lt;br /&gt;INGENTA http://www.ingentaconnect.com
Internal Dual-band Low Profile Antenna for T-DMB/UHF Mobile Handset Applications (2006)

A novel low profile and dual-band antenna for the frequency band of T-DMB (Terrestrial Digital Multimedia Broadcasting) and UHF (Ultra High Frequency) applications are presented. By folding an inverted-L antenna with open stubs, the low profile and dual band property are achieved. To operate over T-DMB (80∼210 MHz) and UHF (470∼810 MHz) frequency bands, the impedance matching with two open stubs is used for the proposed antenna. Radiation patterns are omni-directional for all frequency bands. Details of the proposed antenna as well as the measured results are presented.
Stochastic Gene Expression in a Single Cell (2002)

Clonal populations of cells exhibit substantial phenotypic variation. Such heterogeneity can be essential for many biological processes and is conjectured to arise from stochasticity, or noise, in gene expression. We constructed strains of Escherichia coli that enable detection of noise and discrimination between the two mechanisms by which it is generated. Both stochasticity inherent in the biochemical process of gene expression (intrinsic noise) and fluctuations in other cellular components (extrinsic noise) contribute substantially to overall variation. Transcription rate, regulatory dynamics, and genetic factors control the amplitude of noise. These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation.
ISSLS Prize Winner: Integrating Theoretical and Experimental Methods for Functional Tissue Engineering of the Annulus Fibrosus (2008)

AF cells seeded on nanofibrous scaffolds elaborated an organized, anisotropic AF-like extracellular matrix, resulting in improved mechanical properties. A hyperelastic fiber-reinforced constitutive model characterized the functional evolution of engineered AF constructs, and was used to simulate physiologically relevant loading configurations. Model predictions demonstrated that fibers resist shear even when the shearing direction does not coincide with the fiber direction. Further, the model suggested that the native AF fiber architecture is uniquely designed to support shear stresses encountered under multiple loading configurations.
Distribution High Impedance Fault Detection Utilizing High Frequency Current Components (1982)

This paper describes work performed by Texas A&M University on the detection of high impedance faults on distribution primary conductors. Some grounded distribution primary conductors may exhibit a very low fault current such that they may not be cleared by overcurrent protection. These faults may persist indefinitely, possibly causing a fire hazard or a hazard to humans by contact with an energized line.
Allergy and the Gut (2000)

There have frequently been doubts as to the relevance of food allergy, in particular as far as the involvement of the intestinal tract is concerned. Several studies, however, have confirmed the existence of allergic reactions in the gut, with an estimated prevalence of about 1-2% in adults. Clinical symptoms are unspecific and include nausea, vomiting, abdominal pain, cramping and diarrhea. Intestinal mast cells, as well as intestinal eosinophils, have been shown to be involved in the pathogenesis of food-allergy-related enteropathy. In addition to classical IgE-dependent degranulation, further agonists have been demonstrated for mast cell activation, for example IL-4. The methods used to confirm the diagnosis of intestinal allergy are still insufficient. Until now, blinded oral challenge procedures with food antigens have been accepted as the 'gold standard' in diagnosing food allergy, although these tests have practical problems. Therefore, new test systems have been developed, such as endoscopic provocation tests, that may improve diagnostic procedures. Elimination diet still presents the main basis of therapy. Aspects to be focused on in the future are the role fo IgE-independent allergic mechanisms in intestinal allergy, the impact of cross-reactivity with other allergens and the relationship to other inflammatory bowel diseases such as Crohn's disease, ulcerative colitis, celiac disease and irritable bowel syndrome.
Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study (2011)

Department for Environment, Food and Rural Affairs, Higher Education Funding Council for England, Isaac Newton Trust (University of Cambridge), and the Wellcome Trust.
Biotechnology as Applied to the Oils and Fats Industry (1984)

Abstract Three areas where biotechnology is applicable to the oils and fats industry are described. 1. Biotechnology and oil plants includes the cloning of high‐yielding trains of oil palms to yield progeny in large numbers relatively quickly. Mutation of oilseeds to change the fatty acid composition is also being carried out in various laboratories. 2. Biotechnology with enzymes encompasses the use of microbial lipases for the interesterification of triacylglycerols to form cocoa butter substitutes, as well as the use of lipases to hydrolyse selectively particular fatty acyl esters. Some industrial developments in these areas appear imminent. 3. Biotechnology with micro‐organisms can be seen as an alternative means of producing oils and fats to existing routes though the economics are against such processes being taken up for all but the most expensive oils and fats. A range of oils can, however, be produced by bacteria, algae, yeasts and moulds. Micro‐organisms can also be used to transform alkanes and fatty acids to provide various fatty acid derivatives which could be of value. The biochemistry behind lipid accumulation is described. This provides an understanding of the regulatory enzymes which need to be controlled in order to achieve high accumulations of lipids. From such information, it then becomes possible to consider metabolic manipulation of the organism to improve its fat yield. By extension of these arguments, possible opportunities for genetic engineering into both micro‐organisms and plants then become evident.
Compelling transgenetic evidence for transmission of bovine spongiform encephalopathy prions to humans (1999)

There is growing concern that bovine spongiform encephalopathy (BSE) may have passed from cattle to humans. We report here that transgenic (Tg) mice expressing bovine (Bo) prion protein (PrP) serially propagate BSE prions and that there is no species barrier for transmission from cattle to Tg(BoPrP) mice. These same mice were also highly susceptible to a new variant of Creutzfeldt-Jakob disease (nvCJD) and natural sheep scrapie. The incubation times (approximately 250 days), neuropathology, and disease-causing PrP isoforms in Tg(BoPrP)Prnp(0/0) mice inoculated with nvCJD and BSE brain extracts were indistinguishable and differed dramatically from those seen in these mice injected with natural scrapie prions. Our findings provide the most compelling evidence to date that prions from cattle with BSE have infected humans and caused fatal neurodegeneration.