gebhart/patrepeval huggingface dataset:
Lipopeptide Production in Pseudomonas sp. Strain DSS73 Is Regulated by Components of Sugar Beet Seed Exudate via the Gac Two-Component Regulatory System (2002)

Pseudomonas sp. strain DSS73 isolated from the sugar beet rhizosphere produces the cyclic lipopeptide amphisin, which inhibits the growth of plant-pathogenic fungi. By Tn5::luxAB mutagenesis, we obtained two nonproducing mutant strains, DSS73-15C2 and DSS73-12H8. The gene interrupted by the transposon in strain DSS73-15C2 (amsY) encoded a protein with homology to peptide synthetases that was designated amphisin synthetase. DSS73-12H8 carried the transposon in a regulatory gene encoding a protein with homology to the sensor kinase GacS. Growth of strain DSS73-15C2 (amsY) was impaired during the transition to stationary phase in a minimal medium amended with an exudate of sugar beet seeds. This growth phenotype could be complemented by purified amphisin. Seed exudate further induced expression of bioluminescence from the amsY::luxAB reporter during the transition to stationary phase. This agreed with an increase in amphisin production by the DSS73 wild-type strain during early stationary phase. Amphisin synthesis in DSS73 was strictly dependent on GacS, and even induction by seed exudate depended on a functional gacS locus. Hence, a signal triggering the GacS/GacA two-component system appeared to be present in the seed exudate.
Jovian: a framework for optimizing parallel I/O (2002)

There has been a great deal of recent interest in parallel I/O. We discuss the design and implementation of the Jovian library, which is intended to optimize the I/O performance of multiprocessor architectures that include multiple disks or disk arrays. We also present preliminary performance measurements from benchmarking the Jovian I/O library on the IBM SP1 distributed memory parallel machine for two application templates.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Synthesis of 8-Bromo-1-Methylnaphthalene from o-Bromoacetophenone (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 8-Bromo-1-Methylnaphthalene from o-BromoacetophenoneJOSEF KLEIN and ERNST D. BERGMANNCite this: J. Org. Chem. 1957, 22, 9, 1019–1021Publication Date (Print):September 1, 1957Publication History Published online1 May 2002Published inissue 1 September 1957https://pubs.acs.org/doi/10.1021/jo01360a005https://doi.org/10.1021/jo01360a005research-articleACS PublicationsRequest reuse permissionsArticle Views228Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Molecular beam epitaxy of GaAs on Si-on-insulator (1991)

Epitaxial GaAs films have been grown by molecular beam epitaxy (MBE) on Si-on-insulator (SOI) formed by ion beam synthesis. Rutherford backscattering and channeling, x-ray double-crystal diffraction, and infrared reflection measurements have been used to characterize the epitaxial GaAs films. Experimental results show that the crystal quality of the GaAs films improves markedly towards the GaAs surface for thicker films where the minimum channeling yield drops to 10%. Infrared reflection spectra prove that crystalline GaAs films have been deposited on the SOI structures. Refractive index profiles of the GaAs films on SOI structures can be obtained by computer fitting the interference spectra. The results indicate that the crystal quality of these GaAs films is comparable to similar GaAs films deposited directly on Si by MBE.
T. B. L. Webster: An Introduction to Menander. Pp. viii + 211. Manchester: Manchester University Press, 1974. Cloth, £4·50. (1979)

An abstract is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
Therapy with monoclonal antibodies. An in vivo model for the assessment of therapeutic potential (1992)

A set of rat-human and rat-rat chimeric mAb has been created, all possessing V regions identical in their specificity for the mouse CD8 Ag. In vitro all antibodies were able to block cell-mediated lysis but varied greatly in their capacity to utilize rabbit complement. We examined the ability of these chimeric antibodies to deplete in vivo and established a clear hierarchy. Of the human IgG subclasses, only IgG1, 2, and 3 could fix complement in vitro, yet all (IgG1-4) were remarkably potent at depleting CD8+ PBL in vivo. In contrast, human IgA2 and IgE were ineffective at clearing CD8+ PBL. The vector system used to create these antibodies together with the small doses of antibodies required to deplete in vivo make this a simple and rapid system for testing the effects of different antibody isotypes and mutants. We have shown that a mutant of human IgG1, which is incapable of fixing complement, depletes perfectly well in vivo, whereas an aglycosyl IgG1 mutant is rendered inactive. Our model provides a unique opportunity to study effector functions and motifs that are used by mAb in vivo and will help in the design of improved antibodies for human therapy.
Seamless Scene Segmentation (2019)

In this work we introduce a novel, CNN-based architecture that can be trained end-to-end to deliver seamless scene segmentation results. Our goal is to predict consistent semantic segmentation and detection results by means of a panoptic output format, going beyond the simple combination of independently trained segmentation and detection models. The proposed architecture takes advantage of a novel segmentation head that seamlessly integrates multi-scale features generated by a Feature Pyramid Network with contextual information conveyed by a light-weight DeepLab-like module. As additional contribution we review the panoptic metric and propose an alternative that overcomes its limitations when evaluating non-instance categories. Our proposed network architecture yields state-of-the-art results on three challenging street-level datasets, i.e. Cityscapes, Indian Driving Dataset and Mapillary Vistas.
Deductive Question Answering from Multiple Resources. (2004)

Questions in natural language are answered by consulting multiple sources and inferring answers from information they provide. An automated deduction system, equipped with an axiomatic application-domain theory, serves as the coordinator for the process. Sources include data bases, Web pages, programs, and unstructured text. Answers may contain text or visualizations. Although the approach is domain-independent, many of our experiments have dealt with geographic questions.
Enzymatic histochemistry of mouse kidney in plastic. (1987)

Two-micrometer sections of methacrylate-embedded kidney were used to investigate the enzymatic activities of mouse kidney where the proximal tubule and Bowman's capsule from the same corpuscle were viewed in the same section. Alkaline phosphatase, acid phosphatase, 5'-nucleotidase, gamma-glutamyl transpeptidase, N-acetyl-beta-glucosaminidase, leucine aminopeptidase, alpha-naphthyl butyrate esterase, and adenosine triphosphatase activities were observed in the proximal tubule, but only 5'-nucleotidase, alpha-naphthyl butyrate esterase, and alkaline phosphatase were observed in the squamous portion of the parietal epithelium of Bowman's capsule. The use of methacrylate-embedded tissue allowed more precise localization of enzymatic activity than is possible with most frozen sections. This may provide interesting applications not only for characterization of kidney diseases but also for characterization of other normal and abnormal tissues.
Energy-efficient broadcast and multicast trees for reliable wireless communication (2004)

We define energy-efficient broadband and multicast schemes for reliable communication in multi-hop wireless networks. Unlike previous techniques, the choice of neighbors in the broadband and multicast trees in these schemes, are based not only on the link distance, but also on the error rates associated with the link. Our schemes can be implemented using both positive and negative acknowledgement based reliable broadcast techniques in the link layer. Through simulations, we show that our scheme achieves up to 45% improvement over previous schemes on realistic 100-node network topologies. A positive acknowledgment based implementation is preferred. Our simulations show that the additional benefits of a positive acknowledgement based implementation is marginal (1-2%). Therefore a negative acknowledgement based implementation of our schemes is equally applicable in constructing energy-efficient reliable and multicast data delivery paths.
HIF1α Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells (2014)

This work contributes to a deeper understanding of hypoxic regulatory mechanisms in CSCs and will help devise novel therapies against prostate cancer.
Effects of hydrogen on doping of GaInNAs grown by gas-source molecular beam epitaxy (2000)

In this article, we investigate the effects of hydrogen (H) on doping of GaInNAs grown by gas-source molecular beam epitaxy with a rf plasma nitrogen radical beam source. Incorporating nitrogen (N) into p-Ga0.892In0.108As reduces strain and improves thermal stability. With increasing N concentration, more H, from thermally cracked AsH3, is incorporated alongside N into GaInAs. H has two effects on GaInNAs: acting as an isolated donor and passivating shallow dopants by forming complexes. With increasing N concentration, the free carrier concentration in the as-grown highly Be-doped Ga0.892In0.108NxAs1−x is decreased mainly due to H passivation. Rapid thermal annealing (RTA) increases free carrier concentration due to a reduced H level. The as-grown undoped Ga0.924In0.076N0.030As0.970 sample should be p type without H since N introduces a localized acceptor-like level. Our as-grown undoped sample in n-type 6.9×1015 cm−3 due to charge compensation with H donors. After 700 °C RTA, the film becomes p-type 5.7×1016 cm−3 due to the reduced H donors. For highly Si-doped GaInNAs (∼1×1018 cm−3), H mainly passivates the dopants, so the free carrier concentration is increased after RTA.
Herpes simplex virus (HSV)-specific human T-cell clones recognize HSV glycoprotein D expressed by a recombinant vaccinia virus (1986)

Human cytotoxic T-cell (CTL) clones that lyse autologous cells infected with herpes simplex virus (HSV) type 1 or 2 were generated by stimulating lymphocytes with a recombinant vaccinia virus (recombinant vaccinia-gD-1 virus) that expresses HSV type 1 glycoprotein D (gD-1). Furthermore, CTL clones generated with HSV type 1 or with cloned gD-1 lysed autologous cells infected with the recombinant vaccinia-gD-1 virus. Our findings thus showed that gD serves as a target antigen for human CTLs and that a recombinant vaccinia-gD virus activates HSV-specific human CTL.
Mechanism of Aerobic Decomposition of Angeli's Salt (Sodium Trioxodinitrate) at Physiological pH (2004)

The recent determination that Angeli's salt may have clinical application as a nitrogen oxide donor for treatment of cardiovascular diseases such as heart failure has led to renewed interest in the mechanism and products of thermal decomposition of Angeli's salt under physiological conditions. In this report, several mechanisms are evaluated experimentally and by quantum mechanical calculations to determine whether HNO is in fact released from Angeli's salt in neutral, aerobic solution. The mechanism of product autoxidation is also considered.
High-Q mechanical resonator arrays based on carbon nanotubes (2004)

We present results of the characterization of a nanoelectromechanical signal-processing device based on arrays of carbon nanotubes embedded in RF waveguides. The design, fabrication, and operation of the device will be discussed, including initial RF measurements. Preliminary tests suggest that transmission of an RF signal through the array is associated with the mechanical resonance of the carbon nanotubes.
The construction and implementation of metamodels (1975)

This paper suggests a computationally economical alternative to the common method of using simulation for sensitivity studies, i.e., by making repeated simulation runs with incrementally changed inputs and then observing the magnitudes of the resulting changes in the outputs of interest. Although no theory now exists for constructing the requisite "metamodels" for direct sensitivity analysis, it is possible to construct them, as is illustrated by three examples in the field of operations research.
Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco (2006)

Tobacco (Nicotiana tabacum L.) is a natural allotetraploid derived from the interspecific hybridization between ancestral Nicotiana sylvestris and Nicotiana tomentosiformis. The majority of cultivated tobacco differs from both of its progenitor species in that tobacco typically contains nicotine as the primary alkaloid, in contrast to its two progenitors that accumulate nornicotine in the senescing leaves. However, most, if not all, tobacco cultivars possess an unstable mutation, commonly referred to as the conversion locus, that when activated mediates the conversion of a large percentage of nicotine to nornicotine in the senescing leaf. We have recently identified CYP82E4, a tobacco nicotine N-demethylase gene whose expression was highly induced during senescence in plants that have converted, and CYP82E3, a closely related homolog that exhibited no nicotine N-demethylase activity. In this study, domain swapping and site-directed mutagenesis studies identified a single amino acid change that fully restored nicotine N-demethylase activity to CYP82E3. An examination of the N. tomentosiformis orthologs of CYP82E3 and CYP82E4 revealed that both are functional nicotine N-demethylase genes in N. tomentosiformis. Collectively, our results suggest that a single base pair mutation in CYP82E3 and transcriptional suppression of CYP82E4 played important roles in the evolution of the alkaloid profile characteristic of modern tobacco.
Enhanced Production of Human Mini-Proinsulin in Fed-Batch Cultures at High Cell Density of Escherichia coli BL21(DE3)[pET-3aT2M2] (1997)

Synthesis of recombinant protein (human mini-proinsulin) is investigated in fed-batch cultures at high cell concentration of recombinant Escherichia coli BL21(DE3)[pET-3aT2M2]. Transcription of the recombinant gene is controlled by a T7 promoter system. The human mini-proinsulin is characterized by a C-chain peptide consisting of only nine amino acids, whereas the C-chain peptide of natural human proinsulin is made up of 35 amino acids. It is expressed in a fusion protein with a small fusion partner (a peptide with 18 amino acids) and finally aggregated into insoluble inclusion bodies in cytoplasm of recombinant E. coli. The fermentative production of this small fusion mini-proinsulin may be of great advantage in enhancing the yield of human insulin. To find an optimum induction strategy, effects of various key cultivation variables on the mini-proinsulin production are examined in high cell density fed-batch cultures. No general correlation is found between preinduction specific growth rate and recombinant protein synthesis, which confers a flexibility in choosing the feeding strategy of preinduction media for achieving the high cell density cultures. A culture temperature below 37 degrees C is unfavorable for recombinant gene expression, and the T7-based expression system is almost completely repressed at 30 degrees C. The nutrient glucose and yeast extract concentration in postinduction feed media is optimized by applying a statistical method for medium optimization, i.e. response surface methodology, and an effective amount of inducer molecule (IPTG) is determined to maximize the specific recombinant protein formation. The mini-proinsulin production in E. coli culture is significantly influenced by the volumetric feed rate of postinduction media, which is shown to be closely related to the plasmid copy number in the recombinant cell. Consequently, in a single-stage fed-batch process, the mini-proinsulin concentration is increased up to 7 g/L, approximately 62 wt % of which corresponds to mature human insulin. A two-stage fed-batch fermentation process, with recombinant cell growth occurring at a constant growth rate and constant cell concentration in a growth fermenter and mini-proinsulin production in an induction fermenter, is designed, and its efficacy in increasing volumetric productivity of mini-proinsulin is demonstrated.
Cobra venom factor: structural homology with the third component of human complement. (1984)

The functional analogy between cobra venom factor (CVF), the complement-activating protein in cobra venom, and C3b, the activated form of the third complement component, prompted us to conduct a comparative analysis of structural properties of the two proteins derived from two phylogenetically distant species. We subjected CVF and human C3 and its physiologic cleavage products, C3b and C3c, to a variety of biochemical analyses. We report here structural similarities of these proteins, which include similarities in amino acid composition, far and near UV circular dichroism spectra, secondary structure, band patterns and pI values from isoelectric focusing, immunochemical cross-reactivity, ultrastructural morphology, and amino-terminal amino acid sequences. Analysis of these data reveals that, structurally, CVF resembles C3c more than C3b. We conclude that CVF is not the product of a convergent evolution, but is, in all likelihood, derived from a common C3 ancestor protein.
SecVisor (2007)

We propose SecVisor, a tiny hypervisor that ensures code integrity for commodity OS kernels. In particular, SecVisor ensures that only user-approved code can execute in kernel mode over the entire system lifetime. This protects the kernel against code injection attacks, such as kernel rootkits. SecVisor can achieve this propertyeven against an attacker who controls everything but the CPU, the memory controller, and system memory chips. Further, SecVisor can even defend against attackers with knowledge of zero-day kernel exploits. Our goal is to make SecVisor amenable to formal verificationand manual audit, thereby making it possible to rule out known classes of vulnerabilities. To this end, SecVisor offers small code size and small external interface. We rely on memory virtualization to build SecVisor and implement two versions, one using software memory virtualization and the other using CPU-supported memory virtualization. The code sizes of the runtime portions of these versions are 1739 and 1112 lines, respectively. The size of the external interface for both versions of SecVisor is 2 hypercalls. It is easy to port OS kernels to SecVisor. We port the Linux kernel version 2.6.20 by adding 12 lines and deleting 81 lines, out of a total of approximately 4.3 million lines of code in the kernel.
State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology (2014)

Integrated approaches using different in vitro methods in combination with bioinformatics can (i) increase the success rate and speed of drug development; (ii) improve the accuracy of toxicological risk assessment; and (iii) increase our understanding of disease. Three-dimensional (3D) cell culture models are important building blocks of this strategy which has emerged during the last years. The majority of these models are organotypic, i.e., they aim to reproduce major functions of an organ or organ system. This implies in many cases that more than one cell type forms the 3D structure, and often matrix elements play an important role. This review summarizes the state of the art concerning commonalities of the different models. For instance, the theory of mass transport/metabolite exchange in 3D systems and the special analytical requirements for test endpoints in organotypic cultures are discussed in detail. In the next part, 3D model systems for selected organs – liver, lung, skin, brain – are presented and characterized in dedicated chapters. Also, 3D approaches to the modeling of tumors are presented and discussed. All chapters give a historical background, illustrate the large variety of approaches, and highlight up- and downsides as well as specific requirements. Moreover, they refer to the application in disease modeling, drug discovery and safety assessment. Finally, consensus recommendations indicate a roadmap for the successful implementation of 3D models in routine screening. It is expected that the use of such models will accelerate progress by reducing error rates and wrong predictions from compound testing.
Collaborative monocular SLAM with multiple Micro Aerial Vehicles (2013)

This paper presents a framework for collaborative localization and mapping with multiple Micro Aerial Vehicles (MAVs) in unknown environments. Each MAV estimates its motion individually using an onboard, monocular visual odometry algorithm. The system of MAVs acts as a distributed preprocessor that streams only features of selected keyframes and relative-pose estimates to a centralized ground station. The ground station creates an individual map for each MAV and merges them together whenever it detects overlaps. This allows the MAVs to express their position in a common, global coordinate frame. The key to real-time performance is the design of data-structures and processes that allow multiple threads to concurrently read and modify the same map. The presented framework is tested in both indoor and outdoor environments with up to three MAVs. To the best of our knowledge, this is the first work on real-time collaborative monocular SLAM, which has also been applied to MAVs.
Automatic interpolation and recognition of face images by morphing (2002)

This article presents a new method for generating an optimum image morph field, i.e. an optimum mapping of one image to another image by distorting the brightness and geometry of the former image. The mapping is calculated by maximizing the probability of the morph field in a Bayesian framework. In contrast to other techniques this new method needs no training and is derived based on group invariances and expected singularities, only. An infeasible exhaustive search is replaced by an new iterative approximation approach where, in a neighborhood around the current morph field solution, the probability distribution is approximated by a Gaussian probability distribution for which the most likely solution is evaluated by linear algebra techniques. New views are generated by applying linearly interpolated morph fields to the original reference image. Experiments demonstrate that this approach is well suited to interpolate between different views of a single face image or between images of different persons. Finally, a new face recognition algorithm makes use of the fact that morphs among images of a single person are confined to a five-dimensional subspace within the space of all possible morphs.