gebhart/patrepeval huggingface dataset:
Cho, a second endonuclease involved in Escherichia coli nucleotide excision repair (2002)

Nucleotide excision repair removes damages from the DNA by incising the damaged strand on the 3' and 5' sides of the lesion. In Escherichia coli, the two incisions are made by the UvrC protein, which consists of two functional halves. The N-terminal half contains the catalytic site for 3' incision and the C-terminal half contains the residues involved in 5' incision. The genome of E. coli contains an SOS-inducible gene (ydjQ) encoding a protein that is homologous to the N-terminal half of UvrC. In this paper we show that this protein, which we refer to as Cho (UvrC homologue), can incise the DNA at the 3' side of a lesion during nucleotide excision repair. The incision site of Cho is located 4 nt further away from the damage compared with the 3' incision site of UvrC. Cho and UvrC bind to different domains of UvrB, which is probably the reason of the shift in incision position. Some damaged substrates that are poorly incised by UvrC are very efficiently incised by Cho. We propose that E. coli uses Cho for repair of such damages in vivo. Initially, most of the lesions in the cell will be repaired by the action of UvrC alone. Remaining damages, that for structural reasons obstruct the 3' incision by UvrC, will be repaired by the combined action of Cho (for 3' incision) and UvrC (for 5' incision).
Heart regeneration in adult MRL mice (2001)

The reaction of cardiac tissue to acute injury involves interacting cascades of cellular and molecular responses that encompass inflammation, hormonal signaling, extracellular matrix remodeling, and compensatory adaptation of myocytes. Myocardial regeneration is observed in amphibians, whereas scar formation characterizes cardiac ventricular wound healing in a variety of mammalian injury models. We have previously shown that the MRL mouse strain has an extraordinary capacity to heal surgical wounds, a complex trait that maps to at least seven genetic loci. Here, we extend these studies to cardiac wounds and demonstrate that a severe transmural, cryogenically induced infarction of the right ventricle heals extensively within 60 days, with the restoration of normal myocardium and function. Scarring is markedly reduced in MRL mice compared with C57BL/6 mice, consistent with both the reduced hydroxyproline levels seen after injury and an elevated cardiomyocyte mitotic index of 10-20% for the MRL compared with 1-3% for the C57BL/6. The myocardial response to injury observed in these mice resembles the regenerative process seen in amphibians.
Maytansinoid-Antibody Conjugates Induce Mitotic Arrest by Suppressing Microtubule Dynamic Instability (2010)

Maytansine and its analogues (maytansinoids) are potent microtubule-targeted compounds that inhibit proliferation of cells at mitosis. Antibody-maytansinoid conjugates consisting of maytansinoids (DM1 and DM4) attached to tumor-specific antibodies have shown promising clinical results. To determine the mechanism by which the antibody-DM1 conjugates inhibit cell proliferation, we examined the effects of the cleavable anti-EpCAM-SPP-DM1 and uncleavable anti-EpCAM-SMCC-DM1 conjugates on MCF7 human breast tumor cells. We also examined the effects of the free maytansinoids, maytansine and S-methyl DM1 (a version of DM1 that is stable in cell culture medium), for comparison. Both the conjugates and free maytansinoids potently inhibited MCF7 cell proliferation at nanomolar and subnanomolar concentrations, respectively, by arresting the cells in mitotic prometaphase/metaphase. Arrest occurred in concert with the internalization and intracellular processing of both conjugates under conditions that induced abnormal spindle organization and suppressed microtubule dynamic instability. Microtubule depolymerization occurred only at significantly higher drug concentrations. The results indicate that free maytansinoids, antibody-maytansinoid conjugates, and their metabolites exert their potent antimitotic effects through a common mechanism involving suppression of microtubule dynamic instability.
Randomized Evaluation of Pulse Oximetry in 20,802 Patients; I (1993)

Despite the finding of a few significant inter-group differences, the randomization was well balanced with a high validity of data. The overall postoperative complication rate was similar to that in other recent morbidity and mortality studies.
Benzenesulfonylnitrile oxide. 3. Useful new procedures for generation (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBenzenesulfonylnitrile oxide. 3. Useful new procedures for generationPeter A. Wade and M. Krishna PillayCite this: J. Org. Chem. 1981, 46, 26, 5425–5427Publication Date (Print):December 1, 1981Publication History Published online1 May 2002Published inissue 1 December 1981https://doi.org/10.1021/jo00339a041RIGHTS & PERMISSIONSArticle Views185Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (438 KB) Get e-Alertsclose Get e-Alerts
Nucleotide sequence of a t(14;18) chromosomal breakpoint in follicular lymphoma and demonstration of a breakpoint-cluster region near a transcriptionally active locus on chromosome 18. (1985)

The t(14;18)(q32;21) chromosomal translocation characteristic of follicular lymphomas is the most common cytogenetic abnormality known to be associated with any specific type of hematolymphoid malignancy. A fragment of DNA containing the crossover point between chromosomes 14 and 18 was cloned from the tumor cells of a patient with a follicular lymphoma carrying this translocation. Nucleotide sequence analysis of the breakpoint DNA revealed that the break in chromosome 14 occurred in joining region 4(J4) of the nonfunctional immunoglobulin heavy chain allele. This finding and other structural similarities of the breakpoint with the functional diversity region-joining region (D-J) joint in this lymphoma suggest that D-J recombination enzymes played a role in the mechanism of the t(14;18) translocation. Hybridization analysis of DNA from 40 follicular lymphomas showed that the majority of t(14;18) translocations occur on chromosome 18 DNA within 4.2 kilobases of the cloned breakpoint. A DNA probe from this breakpoint-cluster region detects transcription products in the tumor cells from which it was cloned and in a B-lymphoma cell line containing a t(14;18) translocation.
Minimum-Knowledge Interactive Proofs for Decision Problems (1989)

Interactive communication of knowledge from the point of view of resource-bounded computational complexity is studied. Extending the work of Goldwasser, Micali, and Rackof [Proc. 17th Annual ACM Symposium on the Theory of Computing, 1985, pp. 291–304; .,18 (1989), pp. 186–208], the authors define a protocol transferring the result of any fixed computation to be minimum-knowledge if it communicates no additional knowledge to the recipient besides the intended computational result. It is proved that such protocols may be combined in a natural way so as to build more complex protocols. A protocol is introduced for two parties, a prover and a verifier, with the following properties:(1) Following the protocol, the prover gives to the verifier a proof of the value, 0 or 1, of a particular Boolean predicate, which is (assumed to be) hard for the verifier to compute. Such a deciding “interactive proof-system” extends the interactive proof-systems of [op. cit.], which are used only to confirm that a certain predicate has value 1. (2) The protocol is minimum-knowledge. (3) The protocol is result-indistinguishable: an eavesdropper, overhearing an execution of the protocol, does not learn the value of the predicate that is proved. The value of the predicate is a cryptographically secure bit, shared by the two parties to the protocol. This security is achieved without the use of encryption functions, all messages being sent in the clear. These properties enable one to define a cryptosystem in which each user receives exactly the knowledge he is supposed to receive, and nothing more.
Miniature 3D micro-machined solid state power amplifiers (2008)

In this work, a rectangular coaxial transmission line based power amplifier is discussed. This transmission line is created by sequential photolithography and plating processes, resulting in a very wide band, dispersion-less transmission medium which includes integrated passive components. This approach yields a number of advantages over conventional microstrip circuits. This 3D micro-machining technology, called PolystrataTM technology, in which the circuit is the package, and the package is the circuit, enables one other important and far reaching advantage.
Increased Prostaglandin Production by Human Monocytes after Membrane Receptor Activation (1979)

Human monocytes in culture release small amounts of prostaglandin E (PGE) into the medium. Addition of Fc fragments of IgG to human monocyte monolayer cultures results in a marked increase in PGE release; Fab fragments, monomeric IgG, and human serum albumin have no effect. An IgG1 myeloma has no effect on PGE levels but addition of the heat aggreagted protein results in a marked increase of PGE secretion. Exposure of the cells to Con A, which binds to a specific monocyte plasma membrane receptor, also results in a large increase in PGE release. The magnitude of the increase in PGE secretion produced by exposure of the monocytes to these ligands greatly exceeds the stimulation observed after the addition of antigen-activated mononuclear cell supernatants, zymosan, Sephadex beads, or endotoxin, to monocyte cultures. Prostaglandin E2 (PGE2) accounts for approximately 70% of the total prostaglandins released by stimulated cells. After addition of Indomethacin to monocyte cultures, the stimulatory effects of the ligands on PGE release are inhibited. Addition of Con A to monocyte cultures results in an increased incorporation of [3H]-arachidonic acid into PGE2. These results suggest that this ligand stimulates synthesis as well as release of this prostaglandin.
Super-high-frequency two-port AlN contour-mode resonators for RF applications (2010)

This paper reports on the design and experimental verification of a new class of thin-film (250 nm) superhigh- frequency laterally-vibrating piezoelectric microelectromechanical (MEMS) resonators suitable for the fabrication of narrow-band MEMS filters operating at frequencies above 3 GHz. The device dimensions have been opportunely scaled both in the lateral and vertical dimensions to excite a contour-extensional mode of vibration in nanofeatures of an ultra-thin (250 nm) AlN film. In this first demonstration, 2-port resonators vibrating up to 4.5 GHz have been fabricated on the same die and attained electromechanical coupling, kt(2), in excess of 1.5%. These devices are employed to synthesize the highest frequency MEMS filter (3.7 GHz) based on AlN contour-mode resonator technology ever reported.
Influenza: Emergence and Control (2004)

The natural reservoirs of influenza A viruses are the aquatic birds of the world (91), in which the viruses appear to be in evolutionary stasis—they are in equilibrium with their natural host and cause no disease. Periodically, the influenza viruses are transmitted to other hosts, including mammals, and cause transitory infections and occasionally deaths. Less frequently, influenza viruses are transmitted to other species and establish continual infections in those hosts. Thus, permanent lineages of influenza A viruses occur in humans, swine, horses, and domestic poultry. What is the difference between transitory infection occasionally causing death and the establishment of permanent lineages in the host? The molecular bases of a virus's ability to spread among a range of hosts and of the pathogenicity of influenza viruses are still unresolved. Since 1997, when H5N1 influenza virus was transmitted to humans and killed 6 of 18 infected persons, there have been multiple transmissions of avian influenza viruses to mammals. Either the whole virus is transmitted directly (12, 81) or gene segments from the avian influenza virus are acquired by mammalian strains (e.g., H3N2 triple reassortants in pigs in the United States) (37, 38, 97, 98). Widespread infections of poultry with H5N1 viruses in Asia have caused increasing concern that this subtype may achieve human-to-human spread and establish interspecies transmission. In this minireview, we consider recent interspecies transmissions of influenza A viruses and examine our limited knowledge of the contributors to the success of these viruses. Finally, we briefly consider control measures.
FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment (2007)

Pluripotent embryonic stem (ES) cells must select between alternative fates of self-replication and lineage commitment during continuous proliferation. Here, we delineate the role of autocrine production of fibroblast growth factor 4 (Fgf4) and associated activation of the Erk1/2 (Mapk3/1) signalling cascade. Fgf4 is the major stimulus activating Erk in mouse ES cells. Interference with FGF or Erk activity using chemical inhibitors or genetic ablations does not impede propagation of undifferentiated ES cells. Instead, such manipulations restrict the ability of ES cells to commit to differentiation. ES cells lacking Fgf4 or treated with FGF receptor inhibitors resist neural and mesodermal induction, and are refractory to BMP-induced non-neural differentiation. Lineage commitment potential of Fgf4-null cells is restored by provision of FGF protein. Thus, FGF enables rather than antagonises the differentiation activity of BMP. The key downstream role of Erk signalling is revealed by examination of Erk2-null ES cells, which fail to undergo either neural or mesodermal differentiation in adherent culture, and retain expression of pluripotency markers Oct4, Nanog and Rex1. These findings establish that Fgf4 stimulation of Erk1/2 is an autoinductive stimulus for naïve ES cells to exit the self-renewal programme. We propose that the Erk cascade directs transition to a state that is responsive to inductive cues for germ layer segregation. Consideration of Erk signalling as a primary trigger that potentiates lineage commitment provides a context for reconciling disparate views on the contribution of FGF and BMP pathways during germ layer specification in vertebrate embryos.
Dynamics of Iron Uptake and Fe3O4Biomineralization during Aerobic and Microaerobic Growth ofMagnetospirillum gryphiswaldense (1998)

Iron uptake and magnetite (Fe3O4) crystal formation could be studied in the microaerophilic magnetic bacterium Magnetospirillum gryphiswaldense by using a radioactive tracer method for iron transport and a differential light-scattering technique for magnetism. Magnetite formation occurred only in a narrow range of low oxygen concentration, i.e., 2 to 7 microM O2 at 30 degrees C. Magnetic cells stored up to 2% iron as magnetite crystals in intracytoplasmic vesicles. This extraordinary uptake of iron was coupled tightly to the biomineralization of up to 60 magnetite crystals with diameters of 42 to 45 nm.
VLA-4 Mediated Adhesion to Bone Marrow Stromal Cells Confers Chemoresistance to Adherent Lymphoma Cells (2001)

The mechanisms of maintenance of residual lymphoma in bone marrow during chemotherapy are currently not well understood. Previous studies have shown that primary lymphoma cells obtained from histologically negative bone marrow of non-Hodgkin's lymphoma (NHL) patients grew in long-term bone marrow cultures primarily in association with bone marrow stromal cells. Furthermore, the interaction of NHL patient cells with bone marrow stromal cells inhibited their spontaneous apoptosis. The current studies were designed to characterize the components of the heterotypic interaction between lymphoma cells and bone marrow stromal cells as well as to probe the consequences of this interaction as it pertains to the potential survival of minimal numbers of lymphoma cells during chemotherapy. Cellular adhesion assays performed in the presence of either neutralizing antibodies to VCAM- or the alpha and beta subunit of VLA-4 resulted in >95%, 82% and 35% inhibition of lymphoma cell line adhesion to the bone marrow stromal line MS-5, respectively. Modulation of VLA-4 affinity by the 8A2 antibody resulted in enhanced secondary adhesion at 24 and 72 hours to either cellular fibronectin (65% and 65%) or MS-5 cells (60% and 55%), superceding levels obtained using untreated lymphoma cells (<20%). The bone marrow stromal cells induced a chemoprotective effect for adherent lymphoma cells over a 3-log dose range of vincristine, resulting in a 2-log increase in the ED50 at day 6 of culture. The failure of glutaraldehyde fixed stromal cells to induce a chemoprotective effect demonstrated that viable bone marrow stromal cells were necessary. Similarly, lymphoma/stromal cell conditioned medium also failed to provide a survival advantage. These data demonstrated that viable bone marrow stromal cells possessed the ability to actively inhibit the apoptotic pathways of intimately adherent lymphoma cells and this potentially contributes to their survival during chemotherapy.
Functions of Toll-like receptors: lessons from KO mice (2004)

The innate immune response is a first-line defense system in which individual Toll-like receptors (TLRs) recognize distinct pathogen-associated molecular patterns (PAMPs) and exert subsequent immune responses against a variety of pathogens. TLRs are composed of an extracellular leucine-rich repeat (LRR) domain and a cytoplasmic domain that is homologous to that of the IL-IR family. Upon stimulation, TLR recruits a cytoplasmic adaptor molecule MyD88, then IL-IR-associated kinase (IRAK), and finally induces activation of NF-kappaB and MAP kinases. However, the responses to TLR ligands differ, indicating the diversity of TLR signaling pathways. Besides MyD88, several novel adaptor molecules have recently been identified. Differential utilization of these adaptor molecules may provide the specificity in the TLR signaling.
Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins (2003)

The fusion protein toxins that have been described are generally composed of the catalytic and transmembrane domains of a bacterial toxin (e.g., diphtheria toxin [DT] or Pseudomonas exotoxin A) to which a polypeptide hormone, growth factor, or single-chain antibody (scFv) is genetically fused (1–3). In these constructs, the native receptor binding domain of the toxin is genetically replaced with the targeting ligand. Our laboratory has focused almost exclusively on the construction, expression, and characterization of DT-based fusion toxins (1–12). We have used these novel cytotoxic reagents as probes to study structure-function relationships of the DT catalytic and transmembrane domains, as well as to study the receptor binding components of the fusion proteins (13–19). We are currently using these novel reagents to study the underlying mechanisms involved with delivery of the fusion protein toxin’s catalytic domain across the membrane and into the cytosol of target eucaryotic cells. The catalytic domain of diphtheria toxin catalyzes the NAD+-dependent ADP-ribosylation of the diphthamide residue in elongation factor 2, resulting in inhibition of protein synthesis (20,21). As the delivery of a single molecule of the catalytic domain to the eucaryotic cell cytosol has been shown to result in the death of that cell, the fusion protein toxins represent a family of highly potent, receptor-specific, cytotoxic probes (22).
Scatter Data For Zinc Selenide At 0.633, 3.39, And 10.6 µm (1986)

The bidirectional scattering distribution functions (BSDF&apos;s) of zinc selenide are reported. The scatter in both reflection and transmission modes was measured at 0.6328, 3.39, and 10.6 &mu;m and for several angles of incidence.
Use of the Synera™ Patch for Local Anesthesia Before Vascular Access Procedures: A Randomized, Double-Blind, Placebo-Controlled Study (2007)

A 20-minute application of Synera consistently provided clinically useful anesthesia for vascular access procedures, and appears to be well suited for topical dermal anesthesia due to its reduced time required to produce adequate anesthesia and high subject and investigator acceptance.
Evidence-based approaches to childhood stunting in low and middle income countries: a systematic review (2017)

CRD42016043772.
Initial Antigen Encounter Programs CD8+ T Cells Competent to Develop into Memory Cells That Are Activated in an Antigen-Free, IL-7- and IL-15-Rich Environment (2004)

Although much is known concerning the immunobiology of CD8+ T memory cells, the initial events favoring the generation of CD8+ T memory cells remain poorly defined. Using a culture system that yields memory-like CD8+ T cells, we show that 1 day after Ag encounter, Ag-activated T cells developed into memory-like T cells, but this optimally occurred 3 days after Ag encounter. Key phenotypic, functional, and molecular properties that typify central memory T cells were expressed within 48 h when the activated CD8+ T cells were cultured with IL-7 or IL-15 in the absence of Ag or following transfer into normal mice. These data support a model whereby Ag activation of naive CD8+ T cells not only programs effector cell expansion and contraction but the potential to develop into a memory cell which ensues in an Ag-free environment containing IL-7 or IL-15.
Peak-to-average power ratio reduction of an OFDM signal using partial transmit sequences (2000)

Orthogonal frequency-division multiplexing (OFDM) is an attractive technique for achieving high-bit-rate wireless data transmission. However, the potentially large peak-to-average power ratio (PAP) has limited its application. Recently, two promising techniques for improving the PAP statistics of an OFDM signal have been proposed: the selective mapping and partial transmit sequence approaches. Here, we present suboptimal strategies for combining partial transmit sequences that achieve similar performance but with reduced complexity.
VINCRISTINE NEUROPATHY (1973)

Journal Article VINCRISTINE NEUROPATHY: CLINICAL AND ELECTROPHYSIOLOGICAL OBSERVATIONS Get access E. B. CASEY, E. B. CASEY Search for other works by this author on: Oxford Academic PubMed Google Scholar A. M. JELLIFE, A. M. JELLIFE Search for other works by this author on: Oxford Academic PubMed Google Scholar PAMELA M. LE QUESNE, PAMELA M. LE QUESNE Search for other works by this author on: Oxford Academic PubMed Google Scholar YVONNE L. MILLETT YVONNE L. MILLETT the Departments of Neurological Studies and Radiotherapy, The Middlesex HospitalLondon Search for other works by this author on: Oxford Academic PubMed Google Scholar Brain, Volume 96, Issue 1, March 1973, Pages 69–86, https://doi.org/10.1093/brain/96.1.69 Published: 01 March 1973 Article history Received: 14 June 1972 Published: 01 March 1973