gebhart/patrepeval huggingface dataset:
Effects of the knockdown of death-associated protein 3 expression on cell adhesion, growth and migration in breast cancer cells (2015)

The death-associated protein 3 (DAP3) is a highly conserved phosphoprotein involved in the regulation of autophagy. A previous clinical study by our group suggested an association between low DAP3 expression and clinicopathological parameters of human breast cancer. In the present study, we intended to determine the role of DAP3 in cancer cell behaviour in the context of human breast cancer. We developed knockdown sub-lines of MCF7 and MDA-MB‑231, and performed growth, adhesion, invasion assays and electric cell-substrate impedance sensing (ECIS) studies of post-wound migration of the cells. In addition, we studied the mRNA expression of caspase 8 and 9, death ligand signal enhancer (DELE), IFN-β promoter stimulator 1 (IPS1), cyclin D1 and p21 in the control and knockdown sub-lines. The knockdown sub-lines of MCF7 and MDA-MB‑231 had significantly increased adhesion and decreased growth when compared to the controls. Furthermore, invasion and migration were significantly increased in the MDA-MB-231DAP3kd cells vs. the controls. The expression of caspase 9 and IPS1, known components of the apoptosis pathway, were significantly reduced in the MCF7DAP3kd cells (p=0.05 and p=0.003, respectively). We conclude that DAP3 silencing contributes to breast carcinogenesis by increasing cell adhesion, migration and invasion. It is possible that this may be due to the activity of focal adhesion kinase further downstream of the anoikis pathway. Further research in this direction would be beneficial in increasing our understanding of the mechanisms underlying human breast cancer.
Real-time area-covering operations with obstacle avoidance for cleaning robots (2003)

An area-covering operation is a kind of complete coverage path planning, which requires the robot path to cover every part of the workspace, which is an essential issue in cleaning robots and many other robotic applications such as vacuum, robots, painter robots, land mine detectors, lawn mowers, and windows cleaners. In this paper, a novel biologically inspired neural network approach is proposed for complete coverage path planning with obstacle avoidance of a cleaning robot in a nonstationary environment. The dynamics of each neuron in the topologically organized neural network is characterized by a shunting equation or an additive equation derived from Hodgkin and Huxley's (1952) membrane equation. There are only local lateral connections among neurons. Thus the computational complexity linearly depends on the neural network size. The proposed model algorithm is computationally efficient, and can also deal with changing environment. Simulation results show that the proposed model is capable of planning collision-free complete coverage robot path.
Gene structure and chromosomal localization of the human P2X7 receptor. (1998)

The genomic organization for the human P2X7 receptor gene was determined to comprise 13 exons. Alignment of the exon-intron junctions with those for the rat P2X2 gene demonstrated a precise conservation of the boundaries for the first 10 introns. The human P2X7 receptor gene was localized by in situ hybridization to chromosome 12q24. Radiation hybrid mapping indicated that this is within 130 kb of the gene for the homologous P2X4 receptor.
Identification of Novel Small Molecule Ligands That Bind to Tetanus Toxin (2000)

Tetanus toxin belongs to a family of clostridial protein neurotoxins for which there are no known antidotes. Another closely related member of this family, botulinum toxin, is being used with increasing frequency by physicians to treat severe muscle disorders. Botulinum toxin has also been produced in large quantities by terrorists for use as a biological weapon. To identify small molecule ligands that might bind to the targeting domain of tetanus and botulinum toxins and to facilitate the design of inhibitors and new reagents for their detection, molecular docking calculations were used to screen a large database of compounds for their potential to bind to the C fragment of tetanus toxin. Eleven of the predicted ligands were assayed by electrospray ionization mass spectrometry (ESI-MS) for binding to the tetanus toxin C fragment, and five ligands (45%) were found to bind to the protein. One of these compounds, doxorubicin, was observed to have strong hydrophobic interactions with the C fragment. To check the ligands for their ability to compete with ganglioside binding, each was also tested using a GT1b liposome assay. Doxorubicin was the only ligand found to competitively bind the tetanus toxin C fragment with an appreciable binding constant (9.4 microM).
Conditional ectopic expression of C/EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis. (1995)

Activation of adipogenesis in 3T3 preadipocytes by exposure to the adipogenic inducers dexamethasone, methylisobutylxanthine, insulin, and fetal bovine serum is accompanied by a transient burst of C/EBP beta protein expression that precedes the induction of the fat gene program. In this study we have investigated the role of C/EBP beta in initiating the adipogenic program by overexpressing C/EBP beta in multipotential NIH-3T3 fibroblasts. Conditional ectopic expression of C/EBP beta was accomplished by using an artificial transcriptional regulatory system based on the Escherichia coli tetracycline repressor to generate a stable cell line, beta 2, that expresses C/EBP beta mRNA and protein in a tightly controlled tetracycline dose-dependent manner. Induction of C/EBP beta DNA-binding activity in NIH-3T3 beta 2 cells exposed to dexamethasone in the presence of insulin and fetal bovine serum activates the expression of an adipocyte-specific nuclear hormone receptor, PPAR gamma, that stimulates the conversion of these fibroblasts into committed preadipocytes. Either ectopic expression of C/EBP beta or treatment with dexamethasone alone is incapable of inducing PPAR gamma expression, but when present together, they have a synergistic effect on the adipogenic program. Exposure of these stimulated cells to a PPAR activator 5,8,11,14-eicosatetraynoic acid (ETYA) results in the accumulation of fat droplets and expression of the adipocyte-enriched genes aP2 and glycerol phosphate dehydrogenase (GPD). The number of beta 2 cells that can differentiate into adipocytes is related to the concentration of tetracycline and, therefore, the amount of the exogenous C/EBP beta protein expressed. C/EBP beta can induce PPAR gamma mRNA in the absence of ETYA; however, expression of aP2 mRNA and maximum fat deposition is dependent on the PPAR activator. Our results suggest that enhanced expression of C/EBP beta converts multipotential mesenchymal precursor cells into preadipocytes that respond to adipogenic inducers, including dexamethasone and PPAR activators to differentiate into adipocytes.
Tan cerca y tan lejos: adolescentes en conflicto con la ley. Joao Pessoa (Brasil), Burjassot (España). De la exclusión educativa al gusto por el aprendizaje (2007)

Cuando llegue al aeropuerto de Joao Pessoa (Paraiba)-Brasil, crei que todo lo que iba a acontecer en los proximos dias seria muy novedoso para mi, que iba a conocer nuevas realidades, nuevas formas de comprender la sociedad etc., y en parte asi fue, pero solo en parte, dado que despues del momento inicial y quizas porque tengo mi mirada entrenada o desviada hubo procesos o situaciones que no me parecieron nuevas ni sorpresivas.
Quizas quien lea estas paginas considere que soy un atrevido e un imprudente por escribir de los adolescentes en conflicto con la ley de Joao Pessoa (Paraiba), siendo de tan lejos, y compararlos con los adolescentes en conflicto con la ley de Burjassot (Valencia) pero a veces pienso que determinados procesos sociales estan tan lejos y tan cerca, tan distantes y tan proximos, que voy a permitirme ser osado y escribir sobre ello, aunque solo sea para suscitar una reflexion, un debate, una lectura.
Fundamentalmente centrare la comunicacion en analizar la experiencia previa educativa de los dos grupos de adolescentes investigados, sus encuentros y desencuentros con los sistemas educativos, y sin duda aquello que mas me atrae de ambos grupos y es su interes por el aprendizaje desarrollado en torno a un proyecto de radio comunitaria, en ambos polos del continente, donde los adolescentes se interesan por su comunidad, por las tecnologias, y adquieren competencias y habilidades.
Por otra parte, lo analizado me sugiere una pregunta final que no respondere aunque aportare algunas reflexiones a ella: ?es posible que los sistemas educativos desarrollen estrategias para trabajar con estos adolescentes? ?O debemos de reconocer de forma definitiva que deben ser otros sistemas, otras realidades las que den respuesta a estos jovenes muchos de ellos todavia en edad escolar obligatoria?
Surface tension effect on the mechanical properties of nanomaterials measured by atomic force microscopy (2004)

The effect of reduced size on the elastic properties measured on silver and lead nanowires and on polypyrrole nanotubes with an outer diameter ranging between 30 and 250 nm is presented and discussed. Resonant-contact atomic force microscopy (AFM) is used to measure their apparent elastic modulus. The measured modulus of the nanomaterials with smaller diameters is significantly higher than that of the larger ones. The latter is comparable to the macroscopic modulus of the materials. The increase of the apparent elastic modulus for the smaller diameters is attributed to surface tension effects. The surface tension of the probed material may be experimentally determined from these AFM measurements.
Learning from California`s QF auction (1995)

California`s 1993 qualifying facility (QF) auction dramatically illustrates problems that can be encountered in structuring auctions for electric utility solicitations of supply-side resources from qualifying cogeneration and small power production facilities. In the 1993 California QF auction, three California utilities were to select QFs that would be awarded long-term purchased-power contracts. The auction produced some unexpected outcomes that could potentially cost the utilities and their customers tens of millions of dollars per year. A year after the auction was held, the parties were still attempting to revise contracts, or even rebid a portion of the auction. And all of this effort may come to naught, depending upon what happens now that the Federal Energy Regulatory Commission has struck down the auction process under California`s Biennial Resource Plan Update, claiming that it violates the Public Utility Regulatory Policies Act (PURPA) by using an improper method to calculate avoided costs. A study of the California QF auction illustrates yet again that the devil is in the details. And the stakes will only rise farther as regulators in other states rely more on auctions to open up the electric industry to further competition.
Locally Erasable Couplers for Optical Device Testing in Silicon on Insulator (2014)

Wafer scale testing is critical to reducing production costs and increasing production yield. Here we report a method that allows testing of individual optical components within a complex optical integrated circuit. The method is based on diffractive grating couplers, fabricated using lattice damage induced by ion implantation of germanium. These gratings can be erased via localised laser annealing, which is shown to reduce the outcoupling efficiency by over 20 dB after the device testing is completed. Laser annealing was achieved by employing a CW laser, operating at visible wavelengths thus reducing equipment costs and allowing annealing through thick oxide claddings. The process used also retains CMOS compatibility.
Structure and Immunological Properties of D-Arabino-D-galactans Isolated from Cell Walls of Mycobacterium Species (1974)

Journal Article Structure and Immunological Properties of D-Arabino-D-galactans Isolated from Cell Walls of Mycobacterium Species Get access Akira MISAKI, Akira MISAKI Search for other works by this author on: Oxford Academic PubMed Google Scholar Nobuyo SETO, Nobuyo SETO Search for other works by this author on: Oxford Academic PubMed Google Scholar Ichiro AZUMA Ichiro AZUMA *Third Department of Internal Medicine, School of Medicine, Osaka UniversityOsaka Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 76, Issue 1, July 1974, Pages 15–27, https://doi.org/10.1093/oxfordjournals.jbchem.a130540 Published: 01 July 1974 Article history Received: 12 February 1974 Published: 01 July 1974
CONCEPTS OF SEROMA FORMATION AND PREVENTION IN BREAST CANCER SURGERY (2006)

Evidence suggests an increase in the incidence of seroma because of thermal trauma from electrocautery dissection, but this is indispensable for surgical haemostasis. Obliteration of dead space by various flap apposition techniques has been shown to be advantageous in reducing incidence and volume of seroma. Low-pressure suction drainage reduces seroma volume and duration of drainage leading to earlier drain removal. Preventive measures have to be tailored according to individual patient and operative factors.
A Critical Review of Electromagnetic Heating for Enhanced Oil Recovery (2008)

Abstract A variety of enhanced oil recovery methods have been developed and applied to mature and depleted reservoirs in order to improve the efficiency of primary (pressure deleted) and secondary recovery (water flooding) methods. In thermal recovery methods, cyclic steam and hot water injection, steam and hot water flooding, in situ combustion, electrical heating, and electromagnetic heating are used. However, each of these methods is highly energy intensive. Electromagnetic enhanced oil recovery method is a relatively new method and has been of great interest in the recent years. This article presents a critical review of electromagnetic heating of reservoirs for enhanced oil recovery.
The Versatile MHC Class I-related FcRn Protects IgG and Albumin from Degradation: Implications for Development of New Diagnostics and Therapeutics (2009)

The half-life of the two most abundant proteins in blood, immunoglobulin G (IgG) and serum albumin, is extraordinary (approximately 19-23 days) compared to other circulating proteins. This phenomenon secures a broad biodistribution throughout the body of both molecules. The long half-life has made IgG the natural choice for engineering of antibody based therapeutics, while albumin is used as a fusion partner for or carrier of drugs. Remarkably, the half-life of these unrelated proteins has been shown prolonged by a receptor recycling pathway mediated by a common cell bound receptor named the neonatal Fc receptor (FcRn). This review summarizes our current understanding of FcRn function and discusses its relevance for development of new IgG and albumin based therapeutics and diagnostics.
Absorption and metabolic effects of enterally administered glutamine in humans (1991)

To assess absorption and metabolic effects of enterally delivered glutamine, a total of 10 healthy subjects received perfusions of natural L-glutamine at graded infusion rates (ranging from 0 to 126 mmol/h; n = 2-8 subjects at each rate) along with a nonabsorbable marker (polyethylene glycol) through a double-lumen nasojejunal tube. Perfusions were administered after an overnight fast during three consecutive 1- or 2.5-h periods and in a randomized order. In eight subjects, continuous intravenous infusion of D-[6,6-2H2]glucose, L-[1-13C]leucine, and L-[15N]alanine was simultaneously performed. Glutamine was nearly quantitatively absorbed over the 30-cm study segment; estimated Km and Vmax of glutamine absorption were 2.48 and 2.32 mmol/min over the 30-cm study segment. Enteral glutamine administration induced 1) a dose-dependent increase in plasma glutamine level; 2) a rise in the plasma level and appearance rate (Ra) of alanine (from 191 +/- 42 to 213 +/- 51 mumols.kg-1.h-1, P less than 0.05, for 0 and 46.8-mmol/h glutamine infusion rates, respectively) and in plasma levels of glutamate, citrulline, aspartate, and urea; 3) a decline in plasma free fatty acid and glycerol levels; and 4) no change in leucine or glucose Ra. We conclude that glutamine is efficiently absorbed by human jejunum in vivo and may directly inhibit lipolysis, whereas it neither affects proteolysis nor glucose production in healthy postabsorptive humans.
Status of Cytomegalovirus Prevention and Treatment in 2000 (2000)

Cytomegalovirus (CMV) infection continues to be a problem in selected populations following hematopoietic stem cell transplantation (SCT). Although there have been no new antiviral agents for management of this infection in recent years, the methods for using the existing agents have improved with newer assays for detection of virus. In addition, our understanding of immunity to CMV has undergone considerable expansion. This paper will address these new aspects relating to CMV infection in the setting of SCT. In Section I Dr. Zaia reviews the pathogenesis of CMV and the current epidemiology of CMV disease following marrow or blood allo-SCT with emphasis on late-onset disease. The current lab tests available for preemptive management are summarized including the role for conventional shell vial cultures, and a comparison of the CMV antigenemia assay with the new nucleic acid-based assays, including the hybrid capture assay, the NASBA assay, and "real-time" PCR assays. Use of antiviral agents with these tests in the preemptive management of CMV infection is discussed. Ultimately, what is necessary is restoration of adequate CMV immunity, and that requires understanding the basics of the CMV-specific immune response. In Section II, Dr. Sissons traces the evolution of the CTL response from primary infection into memory and reviews recent advances in the understanding of cytotoxic T cell based immunity to CMV, based on the use of T cell clonotypic analysis and markers of T cell memory and activation, with conventional CTL functional assays. In Section III Dr. Riddell presents approaches to correction of the problem of CMV pathogenesis, namely direct restoration of the CMV-specific cellular immune deficiency. Attempts at passive therapies will be reviewed with the focus on current problems and approaches to these problems. In Section IV, Dr. Diamond presents work on the identification of multiple HLA-allele specific cytotoxic T cell epitopes specific for CMV-pp65 and - pp150. Specific epitopes are recognized by CMV-seropositive individuals including healthy donors, SCT recipients, and AIDS patients, indicating their potential usefulness as vaccines. One of these epitopes is recognized by most individuals who express the HLA A(*)0201 Class I allele. Pre-clinical evaluation in HLA2.1 transgenic mice of vaccine structures utilizing this epitope, and alternative delivery systems are described. Possible methods for vaccination of donor and/or recipient of a SCT as well as their limitations, utilizing synthetic or viral vaccines, are discusseed.
Treatment of Resectable and Locally Advanced Pancreatic Cancer (2000)

The diagnosis and treatment of pancreatic cancer continue to improve although most patients will succumb to their disease. Novel methods of earlier detection and more effective systemic therapies are needed to significantly improve outcomes.
Laboratory Evaluation of Mosquito Repellents AgainstAedes albopictus,Culex nigripalpus, andOchlerotatus triseriatus(Diptera: Culicidae) (2004)

Four synthetic mosquito repellents (Autan [10% KBR3023], IR3535 [7.5%], Off! [15% deet], Skinsations [7% deet]) and eight natural (primarily plant extracts and/or essential oils) product-based repellents (Bite Blocker [2% soybean oil], ByGone, GonE!, Natrapel [10% citronella], Neem Aura, Sunswat, MosquitoSafe [25% geraniol], and Repel [26% p-menthane-3,8-diol]) were tested in the laboratory against Aedes albopictus Skuse, Culex nigripalpus Theobald, and Ochlerotatus triseriatus (Say). When estimated mean protection time (eMPT) responses for each repellent were averaged for all three mosquito species, Autan, Bite Blocker, Off!, and Repel prevented biting for > or =7.2 h; IR3535, MosquitoSafe, and Skinsations for 3.2-4.8 h; and ByGone, Natrapel, GonE, NeemAura, and SunSwat for 0.9-2.3 h. Against Ae. albopictus, the eMPT for Off! and Repel exceeded 7.0 h and ranged from 5.0 to 5.7 h for Autan, Bite Blocker, and Skinsations. Bygone, GonE, NeemAura, and SunSwat provided 0.2 h protection against Ae. albopictus and Oc. triseriatus, whereas Autan, Bite Blocker, Off., and Repel prevented bites by Oc. triseriatus for > or =7.3 h. All 12 repellents provided an eMPT > or =2.8 h against Cx. nigripalpus (maximum: 8.5 h for Bite Blocker). When the average eMPT for each repellent (for all species) was divided by the eMPT for 7% deet (Skinsations), the order of repellent effectiveness and the corresponding repellency index (R,) was Repel (1.7) > Bite Blocker (1.5) = Autan (1.5) = Off! (1.5) > Skinsations (1.0) > IR3535 (0.8) > MosquitoSafe (0.6) > Natrapel (0.5) > Neem Aura (0.3) = SunSwat (0.3) = Bygone (0.3) > GonE (0.2).
The noncatalytic src homology region 2 segment of abl tyrosine kinase binds to tyrosine-phosphorylated cellular proteins with high affinity. (1991)

Several proteins implicated in the regulation of cell proliferation contain a common noncatalytic domain, src homology region 2 (SH2). We have used the bacterially expressed SH2 domain of abl protein-tyrosine kinase to evaluate the ability of this domain to bind to cellular proteins. ablSH2 specifically bound to a number of tyrosine-phosphorylated proteins from cells transformed by tyrosine kinase oncogenes in a filter-binding assay and to a subset of those proteins in solution. The SH2 probe bound almost exclusively to tyrosine-phosphorylated proteins, and binding was eliminated by dephosphorylation of cell proteins. Free phosphotyrosine could partially disrupt SH2 binding, suggesting that phosphotyrosine is directly involved in the binding interaction. These results demonstrate that an SH2 domain is sufficient to confer direct, high-affinity phosphotyrosine-dependent binding to proteins and suggest a general role for SH2 domains in cellular signaling pathways.
SR 33589, a New Amiodarone-like Antiarrhythmic Agent (1995)

We compared the electrophysiological effects of a new amiodarone-like agent, SR 33589, with those of amiodarone. Mongrel dogs were anesthetized with chloralose, and electrodes were implanted in right atrium and ventricle for electrical stimulation and regional ECG measurement. Sinus cycle length (CL), AH interval, Wenckebach CL (WCL), atrial, atrioventricular node, and ventricular effective refractory periods (AERP, AVNERP, VERP), and parameters calculated from surface ECG were measured. SR 33589 was administered intravenously (i.v.) at 1, 2.5, or 5 mg/kg followed 60 min later by a second similar dose. The same protocol was followed with amiodarone 5 mg/kg, which reduced heart rate (HR) by 19% (p < 0.05), increased WCL by 31% (p < 0.01), AH interval by 14% (p < 0.05) and AERP, AVNERP, and VERP by 13% (p < 0.05), 19% (p < 0.05), and 11% (p < 0.01) respectively. No effect was observed on HV or PQ intervals. A second administration of 5 mg/kg changed these indexes further. SR 33589 (2.5 mg/kg) reduced HR by 21% (p < 0.001), increased WCL by 44% (p < 0.001), AH interval by 24% (p < 0.01), and AERP, AVNERP, and VERP by 17% (p < 0.001), 63% (p < 0.01), and 15% (p < 0.01) respectively, with an 18% increase in PQ interval (p < 0.05) but no significant effect on HV interval. Higher doses (5 mg/kg) and/or administration of a second dose both resulted in greater changes. Both amiodarone and SR 33589 prolonged VERP more at longer CL than at shorter CL, but the degree of reduction at shorter CL was less with SR 33589 than with amiodarone. Results suggest that acute administration of SR 33589 results in electrophysiological actions similar to those produced by amiodarone.