gebhart/patrepeval huggingface dataset:
Carcinoembryonic Antigen Cell Adhesion Molecule-1 (CEACAM1) in Posterior Uveal Melanoma: Correlation with Clinical and Histological Survival Markers (2011)

This report describes CEACAM1 expression in uveal melanoma. Correlation with poor prognostic factors such as epithelioid cell type and networks of extracellular matrix pattern was found, but definitive prognostic conclusions still cannot be deduced. Additional validation studies on the use of CEACAM1 expression as a prognostic marker are warranted.
High frequencies of short frameshifts in poly-CA/TG tandem repeats borne by bacteriophage M13 inEscherichia coliK-12 (1987)

Slipped-strand mispairing (SSM) may play an major role in repetitive DNA sequence evolution by generating large numbers of short frameshift mutations within simple tandem repeats. Here we examine the frequency and size spectrum of frameshifts generated within poly-CA/TG sequences inserted into bacteriophage M13 in Escherichia coli hosts. The frequency of detectable frameshifts within a 40 bp tract of poly-CA/TG is greater than one percent and increases more than linearly with length, being lower by a factor of four in a 22 bp target sequence. The frequency increases more than 13-fold in mutL and mutS host cells, suggesting that a high proportion of frameshift events are normally repaired by methyl-directed mismatch repair. Of the 87 sequenced frameshifts in this study, 96% result from deletion or insertion of only or two 2 bp repeat units. The most frequent events are 2 bp deletions, 2 bp insertions, and 4 bp deletions, the relative frequencies of these events being about 18:6:1.
Human Platelet CD36 (GPIIIb, GPIV) Binds to Cholesteryl-Hemisuccinate and Can Be Purified by a Simple Two-Step Method Making Use of this Property (1998)

CD36, also known as GPIIIb or GPIV, is a main protein of the platelet membrane. Accumulating evidence implicates CD36 in the pathogenesis of atherosclerosis. CD36 binds to cholesteryl-hemisuccinate coupled to agarose. This binding may be of physiologic relevance since CD36 has a long extracellular hydrophobic region at its N-terminus and belongs to a family of proteins involved in lipid metabolism. This newly discovered binding property of CD36 was used to develop a rapid, efficient and gentle two-step purification method for CD36 from human platelets.
Real-time Hand Pose Recognition Using Low-Resolution Depth Images (2006)

Gesture recognition methods based on intensity or color images often suffer from low efficiency and lack of robustness. In this paper, we employ a new laser-based camera that produces reliable low-resolution depth images at video rates. By decomposing and recognizing hand poses as finger states (finger poses and finger inter-relations), we achieve robust hand pose recognition in real-time (30 frames/second).
System support for mobility (1996)

As we move closer to world-wide networking and the merger of computing and communications, truly mobile computing---computing while on the move---will become as unexceptional as truly mobile communication (using cellular telephones) is today. However, mobile computing poses new challenges for both system and application designers. Unlike telephony, where a new communications and terminal infrastructure was created to provide a well-understood service (peer-to-peer voice communication), and inter-operability was maintained at the central office, to realize the vision of mobile computing we need to adapt existing applications, and inter-operability must be provided for between peer applications and systems.Almost all of the computer applications that have been built to date make the assumption that their environment is invariant over a significant period of time. Previous generations of bespoke applications were designed around assumptions of computer speed, memory, network bandwidth, communications latency and user interface; changes in these assumptions may have necessitated a new design. Current mass-market ("shrink-wrapped") applications more commonly interrogate their environment when they are initialized, but then assume that nothing changes until after they terminate. Even a modest dynamic change, such as reducing the bit-depth of the available display (as might occur when a laptop is unplugged from a stationary monitor), is more than many applications can handle. Similarly, while operating systems are now often built to ascertain at boot time which network devices are available, and to initialize the appropriate drivers, they are typically unable to handle dynamic changes in these same attributes.The challenge of mobile computing is that many attributes of the application environment vary as the computer is moved from place to place. Moreover, the degree of variability is enormous. For example, available network bandwidth may vary by five orders of magnitude. Adapting to this variability is the shared responsibility of the application program and the operating system. We do not believe that there is a "silver bullet" that will automatically make all applications mobile. But we do believe that the use of the Synthetix specialization methodology will both make the problem tractable and will provide a framework for dividing between the application and the system the responsibility for adapting to change.The remainder of this position paper will describe in more detail the kind of variability that mobile applications will often encounter, and will then outline how we envisage adapting applications and systems to accommodate it.
Optimization of thin film silicon solar cells on highly textured substrates (2011)

Abstract Doped layers made of nanostructured silicon phases embedded in a silicon oxide matrix were implemented in thin film silicon solar cells. Their combination with optimized deposition processes for the silicon intrinsic layers is shown to allow for an increased resilience of the cell design to the substrate texture, with high electrical properties conserved on rough substrates. The presented optimizations thus permit turning the efficient light trapping provided by highly textured front electrodes into increased cell efficiencies, as reported for single junction cells and for amorphous silicon (a‐Si)/microcrystalline silicon tandem cells. Initial and stabilized efficiencies of 12.7 and 11.3%, respectively, are reported for such tandem configuration implementing a 1.1 µm thick microcrystalline silicon bottom cell. magnified image SEM image after FIB cut of an amorphous silicon/microcrystalline silicon tandem cell reported with a stabilized efficiency of 11.3% for a bottom cell thickness of about 1.1 µm.
Overexpressed Csk Tyrosine Kinase Is Localized in Focal Adhesions, Causes Reorganization of αvβ5 Integrin, and Interferes with Hela Cell Spreading (1995)

The C-terminal Src kinase p50csk phosphorylates Src family tyrosine kinases and down-regulates their activity in vitro. To gain insight into the cellular functions of this potentially antioncogenic enzyme, we have overexpressed the csk cDNA by using an inducible promoter in HeLa cells. Despite some differences in basal Src activity in the clones analyzed, Src activity was not significantly suppressed, while the amount of p50csk and Csk activity increased at least 10-fold during 3 days of induction. Immunofluorescence for the induced p50csk was localized in the cytoplasm and distinctly in focal adhesions, in which the amount of phosphotyrosine containing proteins was also increased. Point and deletion mutagenesis experiments showed that localization in focal adhesions was dependent on the SH2 and SH3 domains of Csk but not on its catalytic activity. Csk formed a complex with the focal adhesion protein paxillin in cells, and its SH2 domain was shown to interact with pp125FAK and paxillin in vitro. After Csk induction, the cells became spherical and more loosely attached to the culture substratum, and the alpha v beta 5 integrin complex (vitronectin receptor) of focal adhesions was redistributed to a novel type of structure consisting of punctate plaques on the ventral cell surface. These phenotypic changes occurred in several clones analyzed and were totally reversible when Csk was switched off, but they did not occur in cells overexpressing the catalytically inactive Csk R-222 mutant or luciferase. Our results thus show that a fraction of cellular Csk is targeted to focal adhesions via its SH2 and SH3 domains, probably interacting with tyrosyl-phosphorylated focal adhesion proteins. They also suggest that Csk is involved in the regulation of integrins controlling cell attachment and shape.
Temperature programming in capillary zone electrophoresis (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTemperature programming in capillary zone electrophoresisChen Wen. Whang and Edward S. YeungCite this: Anal. Chem. 1992, 64, 5, 502–506Publication Date (Print):March 1, 1992Publication History Published online1 May 2002Published inissue 1 March 1992https://pubs.acs.org/doi/10.1021/ac00029a010https://doi.org/10.1021/ac00029a010research-articleACS PublicationsRequest reuse permissionsArticle Views146Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Delivery of saporin to human B-cell lymphoma using bispecific antibody: targeting via CD22 but not CD19, CD37, or immunoglobulin results in efficient killing. (1993)

A panel of bispecific F(ab')2 antibodies (BsAb) have been constructed for delivering the ribosome-inactivating protein saporin to human B cell lymphoma. Each derivative was prepared with specificity for saporin and CD19, CD22, CD37, or immunoglobulin. In vitro studies measuring inhibition of [3H]leucine uptake by cultured Daudi and Raji cells demonstrated that, despite all BsAb capturing saporin on the cell surface, BsAb targeting through CD22 were far more cytotoxic than those functioning via CD19, CD37, or surface immunoglobulin. This exceptional activity of the CD22-specific BsAb appears to derive from its ability to deliver and accumulate saporin inside the target cells. Further studies showed that four CD22-specific BsAb all performed with equal potency and were able to increase saporin toxicity (50% inhibitory concentration) up to 1000-fold, from 2 x 10(-7) M to 2 x 10(-10) M. Pairs of anti-CD22 BsAb which recognized different nonblocking epitopes on the saporin molecule were able to bind saporin more avidly to the target cell and, as a consequence, increased cytotoxicity by at least an additional 10-fold, resulting in 50% inhibitory concentration for protein synthesis of 2 x 10(-11) M. These results suggest that selected combinations of BsAb which bind cooperatively to a toxin and the cell surface may provide an efficient way of delivering toxins to unwanted cells in patients.
Axon repulsion from the midline of the Drosophila CNS requires slit function (1999)

Guidance of axons towards or away from the midline of the central nervous system during Drosophila embryogenesis reflects a balance of attractive and repulsive cues originating from the midline. Here we demonstrate that Slit, a protein secreted by the midline glial cells provides a repulsive cue for the growth cones of axons and muscle cells. Embryos lacking slit function show a medial collapse of lateral axon tracts and ectopic midline crossing of ventral muscles. Transgene expression of slit in the midline restores axon patterning. Ectopic expression of slit inhibits formation of axon tracts at locations of high Slit production and misdirects axon tracts towards the midline. slit interacts genetically with roundabout, which encodes a putative receptor for growth cone repulsion.
791. The structure of some diterpenes from Tambooti wood, Spirostachys africana Sond (1962)

W. H. Baarschers, D. H. S. Horn and L. R. F. Johnson, J. Chem. Soc., 1962, 4046 DOI: 10.1039/JR9620004046
Does full-duplex double the capacity of wireless networks? (2014)

Full-duplex has emerged as a new communication paradigm and is anticipated to double wireless capacity. Existing studies of full-duplex mainly focused on its PHY layer design, which enables bidirectional transmission between a single pair of nodes. In this paper, we establish an analytical framework to quantify the network-level capacity gain of full-duplex over half-duplex. Our analysis reveals that inter-link interference and spatial reuse substantially reduces full-duplex gain, rendering it well below 2 in common cases. More remarkably, the asymptotic gain approaches 1 when interference range approaches transmission range. Through a comparison between optimal half- and full-duplex MAC algorithms, we find that full-duplex's gain is further reduced when it is applied to CSMA based wireless networks. Our analysis provides important guidelines for designing full-duplex networks. In particular, network-level mechanisms such as spatial reuse and asynchronous contention must be carefully addressed in full-duplex based protocols, in order to translate full-duplex's PHY layer capacity gain into network throughput improvement.
Plant tissue culture manual : fundamentals and applications (1991)

Tobacco protoplast isolation, culture and regeneration, I. Negrutiu culture, regeneration and transformation of barley protoplasts, P. Lazzeri et al in vitro fertilization of maize, E. Kranz monoclonal antibodies against marker protein for somatic embryogenesis, S. Stirn and H-J. Jacobsen endosperm culture, B.M. Johri and P.S. Srivastava.
Hydrogen-bonded polymer blends (2001)

This thesis discusses three topics in the general area of hydrogen bonded polymer blends. The first pertains to the blending of flame retardant polyphosphazenes. Poly[bis(n-alkyoxy)phosphazenes] blends with poly(butyl methacrylate-co-4-vinyl phenol) (BMAVPh) were initially studied. These results were compared to BMAVPh blends of analogous poly (vinyl n-alkyl ethers) and the phase behavior was similar. Next, poly[bis(carboxylatophenoxy)phosphazene] blends with a structural polyurethane foam were prepared via reactive mixing. The combustion behavior of these foams was analyzed qualitatively, by a horizontal flame test, and quantitatively, by oxygen index (OI) measurements. Both of these tests indicated a modest increase in flame resistance at loadings of 20 wt% and above. In the second topic, equilibrium constants determined from low molecular weight mixtures were used to successfully predict the phase behavior of analogous polymer blends. Due consideration was given to intramolecular screening and functional group accessibility, factors that are a direct consequence of chain connectivity. In the third topic, polymer blends involving an alternating 1:1 copolymer of tetrafluoroethylene (TFE) and a hexafluoroisopropanol modified vinyl ether (HFIPVE) were studied. This copolymer is interesting for both experimental and theoretical studies of the phase behavior of polymer blends because (1) it is amorphous and has a relatively low glass transition temperature (12°C); (2) it has a relatively low solubility parameter (? 7 (cal.cm-3)-0.5); (3) it is soluble in moderately polar solvents, and (4) it contains the hexafluoroisopropanol group that is a strong hydrogen bond donor. Experimental infrared and thermal analysis studies of polymer blends with (co)polymers containing acetoxy, methacrylate and aliphatic ether groups were studied and compared to theoretical predictions of miscibility maps.
Fast free-form deformable registration via calculus of variations (2004)

In this paper, we present a fully automatic, fast and accurate deformable registration technique. This technique deals with free-form deformation. It minimizes an energy functional that combines both similarity and smoothness measures. By using calculus of variations, the minimization problem was represented as a set of nonlinear elliptic partial differential equations (PDEs). A Gauss-Seidel finite difference scheme is used to iteratively solve the PDE. The registration is refined by a multi-resolution approach. The whole process is fully automatic. It takes less than 3 min to register two three-dimensional (3D) image sets of size 256 x 256 x 61 using a single 933 MHz personal computer. Extensive experiments are presented. These experiments include simulations, phantom studies and clinical image studies. Experimental results show that our model and algorithm are suited for registration of temporal images of a deformable body. The registration of inspiration and expiration phases of the lung images shows that the method is able to deal with large deformations. When applied to the daily CT images of a prostate patient, the results show that registration based on iterative refinement of displacement field is appropriate to describe the local deformations in the prostate and the rectum. Similarity measures improved significantly after the registration. The target application of this paper is for radiotherapy treatment planning and evaluation that incorporates internal organ deformation throughout the course of radiation therapy. The registration method could also be equally applied in diagnostic radiology.
Re: Mind: A mobile application for bipolar disorder patients (2014)

Several personal healthcare monitoring systems have been proposed to target somatic diseases and specific mental illness. This paper reports on the re: Mind system, which is a helpful tool that supports the treatment of people diagnosed with bipolar disorder. We developed the system as a hybrid mobile application to help bipolar patients self-monitor a set of parameters that are known to affect their illness while also allowing them to communicate with their physician. Based on data collected from medical personnel, clinicians, patients, patients' relatives and persons akin to them, we created a user-friendly application while making it possible for the patients to customize the application to fit their specific needs. Preliminary testing with patients confirms the usefulness and effectiveness of our current application.
A uniform type structure for secure information flow (2002)

The π-calculus is a formalism of computing in which we can compositionally represent dynamics of major programming constructs by decomposing them into a single communication primitive, the name passing. This work reports our experience in using a linear/affine typed π-calculus for the analysis and development of type systems of programming languages, focussing on secure information flow analysis. After presenting a basic typed calculus for secrecy, we demonstrate its usage by a sound embedding of the dependency core calculus (DCC) and by the development of a novel type discipline for imperative programs which extends both a secure multi-threaded imperative language by Smith and Volpano and (a call-by-value version of) DCC. In each case, the embedding gives a simple proof of noninterference.
In Vitro Characterization of A-315675, a Highly Potent Inhibitor of A and B Strain Influenza Virus Neuraminidases and Influenza Virus Replication (2002)

A-315675 is a novel, pyrrolidine-based compound that was evaluated in this study for its ability to inhibit A and B strain influenza virus neuraminidases in enzyme assays and influenza virus replication in cell culture. A-315675 effectively inhibited influenza A N1, N2, and N9 and B strain neuraminidases with inhibitor constant (K(i)) values between 0.024 and 0.31 nM. These values were comparable to or lower than the K(i) values measured for oseltamivir carboxylate (GS4071), zanamivir, and BCX-1812, except for the N1 enzymes that were found to be the most sensitive to BCX-1812. The time-dependent inhibition of neuraminidase catalytic activity observed with A-315675 is likely due to its very low rate of dissociation from the active site of neuraminidase. The half times for dissociation of A-315675 from B/Memphis/3/89 and A/Tokyo/3/67 (H3N2) influenza virus neuraminidases of 10 to 12 h are significantly slower than the half times measured for oseltamivir carboxylate (33 to 60 min). A-315675 inhibited the replication of several laboratory strains of influenza virus in cell culture with potencies that were comparable or superior to those for oseltamivir carboxylate and BCX-1812, except for the A/H1N1 viruses that were found to be two- to fourfold more susceptible to BCX-1812. A-315675 and oseltamivir carboxylate exhibited comparable potencies against a panel of A/H1N1 and A/H3N2 influenza virus clinical isolates, but A-315675 was found to be significantly more potent than oseltamivir carboxylate against the B strain isolates. The favorable in vitro results relative to other clinically effective agents provide strong support for the further investigation of A-315675 as a potential therapy for influenza virus infections.
Real world data: an opportunity to supplement existing evidence for the use of long-established medicines in health care decision making (2018)

Evidence from medication use in the real world setting can help to extrapolate and/or augment data obtained in randomized controlled trials and establishes a broad picture of a medication's place in everyday clinical practice. By supplementing and complementing safety and efficacy data obtained in a narrowly defined (and often optimized) patient population in the clinical trial setting, real world evidence (RWE) may provide stakeholders with valuable information about the safety and effectiveness of a medication in large, heterogeneous populations. RWE is emerging as a credible information source; however, there is scope for enhancements to real world data (RWD) sources by understanding their complexities and applying the most appropriate analytical tools in order to extract relevant information. In addition to providing information for clinicians, RWE has the potential to meet the burden of evidence for regulatory considerations and may be used in approval of new indications for medications. Further understanding of RWD collection and analysis is needed if RWE is to achieve its full potential.
Induction of cancer cell-specific death via MMP2 promoterdependent Bax expression (2009)

Controlled gene expression in specific cells is a valuable tool for gene therapy. We attempted to determine whether the lentivirus-mediated Tet-On inducible system could be applied to cancer gene therapy. In order to select the genes that induce cancer cell death, we compared the ability of the known pro-apoptotreic genes, Bax and tBid, and a cell cycle inhibitor, p21cip1/waf1, and determined that Bax was the most effective. For the cancer cell-specific expression of rtTA2(S)-M2, we tested the matrix metalloproteinase-2 (MMP-2) promoter and determined that it is highly expressed in cancer cell lines, including SNU475 cells. The co-transduction of two lentiviruses that contain sequences for TRE-Bax and rtTA2(S)-M2, the expression of which is controlled by the MMP-2 promoter, resulted in the specific cell death of SNU475, whereas other cells with low MMP-2 expression did not evidence significant cell death. Our data indicate that the lentivirus-mediated Tet-On system using the cancer-specific promoter is applicable for cancer gene therapy.