gebhart/patrepeval huggingface dataset:
In Vivo Inhibition of Anti-Hepatitis B Virus Core Antigen (HBcAg) Immunoglobulin G Production by HBcAg-Specific CD4+Th1-Type T-Cell Clones in a hu-PBL-NOD/SCID Mouse Model (2001)

Hepatitis B virus (HBV) core antigen (HBcAg)-specific CD4(+) T-cell responses are believed to play an important role in the control of human HBV infection. In the present study, HBcAg-specific, HLA-DR13*-restricted CD4(+) Th1-type T-cell clones were generated which secreted both gamma interferon and tumor necrosis factor alpha after in vitro antigen stimulation. These HBcAg-specific CD4(+) Th1-type T cells were able to lyse HBc peptide-loaded Epstein-Barr virus-transformed lymphoblastoid target cells in vitro. To examine whether these HLA-DR13*-restricted human CD4(+) Th1 T cells also display the same cytotoxic effects in vivo, we transferred peripheral blood leukocytes (PBL) derived from HBV-infected donors or an HBV-naïve donor sharing the DR13*, together with the HBcAg-specific CD4(+) Th1-type T cells and HBcAg, directly into the spleen of optimally conditioned Nod/LtSz-Prkdc(scid)/Prkdc(scid) (NOD/SCID) mice. The production of both secondary anti-HBc-immunoglobulin G (anti-HBc-IgG) and primary HBcAg-binding IgM in hu-PBL-NOD/SCID mice was drastically inhibited by HBcAg-specific CD4(+) Th1-type T cells. No inhibition was observed when CD4(+) Th1 cells and donor PBL did not share an HLA-DR13. These results suggest that HBcAg-specific CD4(+) Th1 T cells may be able to lyse HBcAg-binding, or -specific, B cells that have taken up and presented HBcAg in a class II-restricted manner. Thus, HBcAg-specific CD4(+) Th1-type T cells can modulate the function and exert a regulatory role in deleting HBcAg-binding, or -specific, human B cells in vivo, which may be of importance in controlling the infection.
Marla C(H)ristina Speaks: Latina Identity and the Poetic Dialogue Between Sandra Maria Esteves and Luz Maria Umpierre (1997)

We designed and prepared the new C60 fullerene hybrids bearing a xanthine moiety as potential double-action anti-inflammatory agents, capable of simultaneous inhibition of LPS-induced NO and TNF-alpha production. The 10 microM of fulleropyrrolidine-xanthine dyad 2a and b were effective in suppressing LPS-induced NO production by 55.1+/-2.1% and 58.6+/-2.6%, respectively, but only 2b was also effectively in suppressing LPS-induced TNF-alpha production by 34.0+/-2.7%. We believed that the agents synthesized herein would hold promise for future development of a new generation of potent anti-inflammatory agents.
Generation of chimeric C5a/formyl peptide receptors: towards the identification of the human C5a receptor binding site (1994)

We employed the polymerase chain reaction to produce a series of chimeric C5a/formyl peptide receptors. Chinese hamster ovary cells transfected with these constructs were tested for their ability to bind C5a. Substitution of three of the extracellular domains of the C5a receptor with the corresponding domains of the formyl peptide receptor abolished C5a binding, whilst replacement of the first extracellular loop of the C5a receptor with that of the formyl peptide receptor had little effect on the affinity of the receptor for C5a. We therefore conclude that this first outer loop of the C5a receptor does not participate in ligand binding, whilst involvement of the other extracellular domains of the receptor cannot be ruled out.
Plastics from an Improved Canola Protein Isolate: Preparation and Properties (2010)

Abstract Canola meal proteins were solubilized from canola flour at pH 12 using sodium hydroxide solution. Proteins were then precipitated sequentially at pH values ranging from 11 to 3 in decrements of 1 pH unit. The weight distribution and the properties of these fractions were analyzed. The majority (&gt;76%) of the recovered proteins were precipitated at pH values at or below 7. Another substantial fraction was precipitated at pH 11. The functional and thermal property (differential scanning calorimetry) analyses showed that this protein fraction exhibits the highest water holding capacity and lowest melting point. The plastics prepared with refined protein isolates (with pH 11 fraction removed) showed higher water resistance, tensile, and flexural strength, toughness, and elongation values compared to those prepared with standard canola protein isolates. This shows that mechanical and water resistance properties of protein‐based plastics can be enhanced using improved protein isolates.
Multiple pathways regulate MeCP2 expression in normal brain development and exhibit defects in autism-spectrum disorders (2004)

Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in MECP2, encoding methyl-CpG-binding protein 2 (MeCP2). Although MECP2 is ubiquitously transcribed, MeCP2 expression is developmentally regulated and heterogeneous in neuronal subpopulations, defined as MeCP2(lo) and MeCP2(hi). To test the hypothesis that pathways affecting MeCP2 expression changes may be defective in RTT, autism and other neurodevelopmental disorders without MECP2 mutations, a high-throughput quantitation of MeCP2 expression was performed on a tissue microarray containing frontal cortex samples from 28 different patients with neurodevelopmental disorders and age-matched controls. Combined quantitative analyses of MeCP2 protein and alternatively polyadenylated transcript levels were performed by laser scanning cytometry and tested for significant differences from age-matched controls. Normal cerebral samples showed an increase in total MeCP2 expression and the percentage of MeCP2(hi) cells with age that could be explained by increased MECP2 transcription within the MeCP2(hi) population. A significant decrease in the relative usage of the long transcript in the MeCP2(lo) population was observed in postnatal compared to fetal brain, but alternate polyadenylation did not correlate with MeCP2 expression changes at the single cell level. Brain samples from several related neurodevelopmental disorders, including autism, pervasive developmental disorder, Prader-Willi and Angelman syndromes showed significant differences in MeCP2 expression from age-matched controls by apparently different transcriptional and post-transcriptional mechanisms. These results suggest that multiple pathways regulate the complex developmental expression of MeCP2 and are defective in autism-spectrum disorders in addition to RTT.
Localized Temperature and Chemical Reaction Control in Nanoscale Space by Nanowire Array (2011)

We introduce a novel method for chemical reaction control with nanoscale spatial resolution based on localized heating by using a well-aligned nanowire array. Numerical and experimental analysis shows that each individual nanowire could be selectively and rapidly Joule heated for local and ultrafast temperature modulation in nanoscale space (e.g., maximum temperature gradient 2.2 K/nm at the nanowire edge; heating/cooling time < 2 μs). By taking advantage of this capability, several nanoscale chemical reactions such as polymer decomposition/cross-linking and direct and localized hydrothermal synthesis of metal oxide nanowires were demonstrated.
Tetherless thermobiochemically actuated microgrippers (2009)

We demonstrate mass-producible, tetherless microgrippers that can be remotely triggered by temperature and chemicals under biologically relevant conditions. The microgrippers use a self-contained actuation response, obviating the need for external tethers in operation. The grippers can be actuated en masse, even while spatially separated. We used the microgrippers to perform diverse functions, such as picking up a bead on a substrate and the removal of cells from tissue embedded at the end of a capillary (an in vitro biopsy).
Rapid appearance of hypomethylated DNA in livers of rats fed cancer-promoting, methyl-deficient diets. (1989)

Prolonged intake of diets deficient in sources of methyl groups leads to development of hepatomas in rats and promotes chemical carcinogenesis in both rats and certain strains of mice. Since methylation of cytosine residues in regulatory regions can affect gene activity, several investigators have postulated that the effects of methyl-deficient diets on tumorigenesis result from the inability of cells to maintain normal patterns of DNA methylation. However, significant decreases in the 5-methylcytosine content of liver DNA have not been reported to occur until rats have consumed methyl-deficient diets for several months. To determine whether methyl-deficient diets have immediate effects on nucleic acid methylation, we assessed the degree to which hepatocyte DNA and tRNA were methylated in vivo, by measuring their ability to act as methyl acceptors in vitro. Hypomethylation of DNA and tRNA was detected within 1 week after rats were started on a diet deficient In methionine, choline, folic acid, and vitamin B12 and it persisted throughout the 4 weeks of study. A significant elevation in liver DNA synthesis occurred in parallel with increased hypomethylation of DNA. Chronic failure to fully methylate DNA that is newly synthesized in response to liver damage induced by methyl-deficient diets provides a feasible mechanism for changing patterns of DNA methylation. Our results indicate that such changes could occur rapidly enough to play a causal role in the cancer-promoting and, in some instances, cancer-inducing properties of the diet.
THE EFFECT OF DIETARY FATS ON THE BLOOD LIPIDS AND THEIR RELATION TO ISCHAEMIC HEART DISEASE (1958)

Journal Article THE EFFECT OF DIETARY FATS ON THE BLOOD LIPIDS AND THEIR RELATION TO ISCHAEMIC HEART DISEASE Get access B. BRONTE-STEWART, M.D. M.R.C.P. B. BRONTE-STEWART, M.D. M.R.C.P. Department of the Regius Professor of Medicine The Radcliffe InfirmaryOxford Search for other works by this author on: Oxford Academic PubMed Google Scholar British Medical Bulletin, Volume 14, Issue 3, September 1958, Pages 243–252, https://doi.org/10.1093/oxfordjournals.bmb.a069691 Published: 01 September 1958
High-mobility thin-film transistors based on aligned carbon nanotubes (2003)

Thin-film transistors based on aligned carbon nanotubes (CNTs) were fabricated by directly growing highly ordered CNTs on silicon dioxide surface. The transistor shows a pronounced field effect. Electric transport through the aligned carbon nanotubes is dominated by the holes at room temperature. A hole mobility (μp) of the CNT thin-film transistor was estimated to be as high as 61.6 cm2/V s. Such a mobility is comparable to that of heavily doped n-Si and is larger than individual CNT field-effect transistor. Thus, it reveals a potential application of the aligned CNTs in electronics.
Modulation of B‐cell activation by the B subunit of Escherichia coli enterotoxin: receptor interaction up‐regulates MHC class II, B7, CD40, CD25 and ICAM‐1 (1997)

The B subunits of cholera toxin (CtxB) and Escherichia coli heat-labile enterotoxin (EtxB) are non-toxic lectins that bind and cross-link a ubiquitous cell glycolipid receptor, ganglioside GM1, and are recognized as potent mucosal and systemic immunogens. Here we examine the role of EtxB receptor occupancy in modulating the activation of B cells, in vitro, in primary lymphocyte cultures containing B and T cells. When 48-hr spleen cell cultures containing EtxB were compared with those in the presence of a non-receptor binding mutant, EtxB(G33D), a marked shift in the ratio of CD4+ T cells: B cells was noted. Evidence suggested that this was the result of either enhanced survival or proliferation of B cells associated with receptor occupancy by EtxB. Investigation revealed that EtxB induced only a minimal increase in proliferation above that of EtxB(G33D), in mixed cell cultures, and failed to induce any cell division of purified B cells or T cells. In contrast, receptor-binding by EtxB markedly up-regulated the expression of major histocompatability complex (MHC) class II, B7, intracellular adhesion molecule-1 (ICAM-1), CD40 and CD25 on the B-cell surface. These results indicate that the polyclonal effects of EtxB on B cells are not associated with wide-scale proliferation, but more likely with maintenance of B-cell survival by activation of molecules essential for B-cell differentiation. The findings also highlight the essential role of GM1-interaction with EtxB in the regulation of lymphocyte responses.
[On the electrolytic separation factor of tritium (author's transl)]. (1981)

The electrolytic enrichment process of tritium is in current use for the measurement of its content in natural water. The electrolytic separation factor of tritium which can have experimental values ranging from 4 to 30 is affected by the nature of the cathode, by the electric current density, and by the nature of the electrolyte. It was recently shown that the factor took a value within a limited range when an alkaline solution was electrolyzed by the use of a nickel-cathode cell under a current density around 50 mA/cm2. To re-examine the reproducibility of the separation factor of tritium, the electrolytic enrichment experiment was made using nickel-nickel electrode cells, 0.05 N-alkaline solutions, and a current density of 50 mA/cm2. The observed separation factors were within the range previously reported on nickel-cathode cells. The results, after they were corrected for the carry-over of water vapor and spray droplets, were close to the thermodynamic equilibrium constants for the protium-tritium exchange reaction between liquid water and hydrogen gas at several solution temperatures in the cell ranging from 12 degree C to 33 degree C. The equilibrium conditions would be expected to prevail and to make the factor reproducible, so far as the above conditions were used. At the current densities above 50 mA/cm2 up to 100 mA/cm2, on the other hand, the factors obtained in this work were slightly more than that corresponding to equilibrium, and had a tendency to rise with increase of current density. It is considered that slow exchange by rate process are apt to take place partly at the cathode surface under the condition of such high current strength.
Does sacral nerve stimulation improve global pelvic function in women? (2013)

A Web-based electronic pelvic-floor assessment questionnaire has demonstrated global improvement in pelvic floor function in bowel, urinary, vaginal and sexual dimensions in women following sacral neuromodulation for faecal incontinence.
Corona protein composition and cytotoxicity evaluation of ultra-small zeolites synthesized from template free precursor suspensions (2013)

The toxicity of two types of ultra-small zeolites (8-18 nm) with LTL-and EMT-type structures is reported. Both the LTL- and EMT-type zeolites belong to the same group of molecular sieves; they have large pores (7.1-7.5 angstrom) and low silica content (Si/Al = 1.2-2.3). The zeolites are prepared by an environmentally friendly synthetic approach from precursor suspensions without using any organic template. Cellular interactions with the two types of zeolite nanocrystals are evaluated by cell viability, reactive oxygen species and cell life cycle assays. It is found that various concentrations of zeolites have negligible effects on the cell life cycle. Moreover, the LTL- and EMT-types zeolites did not cause extensive oxidative stress on the cells. Although it is seen that the zeolites extensively entered in the cells, there is no sign of toxicity for all employed concentrations of ultra-small EMT and LTL zeolites. Additionally, no abnormality in DNA replication while exposed to the zeolites is observed. Very importantly, the zeolite corona shows a high affinity for fibrinogen, moderate affinity for apoA-II and complement factor 3, and trace affinity for albumin, which is the most abundant protein of human plasma. Thus the zeolite nanoparticles can be considered as very promising material for purification of fibrinogen and lipoproteins. Since fibrinogen is considered as acute phase protein and found to be the most associated biomolecule in the composition of corona at the surface of zeolites, we propose that these nanoparticles can be potentially pro-inflammatory for in vivo applications
Energy cost of walking of amputees: the influence of level of amputation. (1976)

A comparison of selected gait parameters and the energy cost of prosthetic walking was made in seventy patients with unilateral traumatic and vascular amputations. Amputations above the knee, below the knee, and at the Syme's level were compared in both groups of amputees, and a control group of forty normal subjects also studied. In both groups of amputees performance was significantly better the lower the level of the amputation. When preservation of function is the chief concern, amputation should be performed at the lowest possible level.
An Efficient and Scalable Synthesis of the Endothelin Antagonists UK-350,926 and UK-349,862 Using a Dynamic Resolution Process (2005)

The development and scale-up of a potential manufacturing route to the endothelin antagonists UK-350,926 1 and UK-349,862 2 are described. A key synthetic challenge in designing an efficient route to these molecules was the optical lability of the stereogenic centre during the construction of the acylsulfonamide functionality. In the discovery synthesis of UK-350,926 the chiral centre was introduced by classical resolution and the acylsulfonamide functionality synthesized by construction of the N-sulfonyl bond. An alternative more efficient process route was developed involving the preparation of racemic UK-350,926 and final step dynamic resolution with (S)-(−)-1-phenylethylamine as the key step. The process route prepared the acylsulfonamide by construction of the N-carbonyl bond, eliminates a cryogenic reaction and a hazardous intermediate from the synthesis, improves the overall process yield, and allows access to both endothelin antagonists from common intermediates without the need for purification by chromatography. Full experimental details of the new five-step process to prepare UK-349,862 from commercially available starting materials are given for the first time.
Literacy and Deaf Students in Taiwan: Issues, Practices and Directions for Future Research: Part I (2014)

We describe the literacy issues facing young deaf and hard of hearing (DHH) learners in Taiwan who are learning both a logographic and an alphabetic script. We describe the contextual factors that surround their reading and writing processes such as island demographics, reading achievement levels, and background language learning variables including deaf culture. We then describe and provide graphic illustrations and examples of the linguistic features of the written languages that DHH children are learning (Chinese and English) as well as the sign codes (Signed Chinese, Zhuyin finger alphabet, character signs, palm writing, and air writing) and the Zhuyin Fuhao written visual symbols. We suggest that contextual, cultural and linguistic factors need to be considered in the understanding of how DHH children learn to read and write as well as how they use their sign language and sign codes to build Chinese and English literacy skills.
Proteomic approaches in biological and medical sciences: principles and applications. (2008)

After the first introduction of the concept of "proteome" more than 10 years ago, large-scale studies of protein expression, localization, activities and interactions have gained an exponential increase of interest, leading to extensive research and technology development. Proteomics is expansively applied in many areas, ranging from basic research, various disease and malignant tumors diagnostic and biomarker discovery to therapeutic applications. Several proteomics approaches have been developed for protein separation and identification, and for the characterization of protein function and structure. Two-dimensional gel electrophoresis, chromatography, capillary electrophoresis and mass spectrometry have become the most used proteomics methods. These techniques are also under constant development. This review provides an overview of the main techniques and their combinations, used in proteomics. The emphasis is made on description of advantages and disadvantages of each technique, to navigate in selection of the best application for solving a specific problem.
Privacy through Fake yet Semantically Real Traces (2015)

Camouflaging data by generating fake information is a well-known obfuscation technique for protecting data privacy. In this paper, we focus on a very sensitive and increasingly exposed type of data: location data. There are two main scenarios in which fake traces are of extreme value to preserve location privacy: publishing datasets of location trajectories, and using location-based services. Despite advances in protecting (location) data privacy, there is no quantitative method to evaluate how realistic a synthetic trace is, and how much utility and privacy it provides in each scenario. Also, the lack of a methodology to generate privacy-preserving fake traces is evident. In this paper, we fill this gap and propose the first statistical metric and model to generate fake location traces such that both the utility of data and the privacy of users are preserved. We build upon the fact that, although geographically they visit distinct locations, people have strongly semantically similar mobility patterns, for example, their transition pattern across activities (e.g., working, driving, staying at home) is similar. We define a statistical metric and propose an algorithm that automatically discovers the hidden semantic similarities between locations from a bag of real location traces as seeds, without requiring any initial semantic annotations. We guarantee that fake traces are geographically dissimilar to their seeds, so they do not leak sensitive location information. We also protect contributors to seed traces against membership attacks. Interleaving fake traces with mobile users' traces is a prominent location privacy defense mechanism. We quantitatively show the effectiveness of our methodology in protecting against localization inference attacks while preserving utility of sharing/publishing traces.
Brain‐Derived Neurotrophic Factor Protects against Multiple Forms of Brain Injury in Bacterial Meningitis (2004)

BDNF efficiently reduces multiple forms of neuronal injury in bacterial meningitis and may hold promise as adjunctive therapy for this disease.
Thioredoxin-Interacting Protein (2008)

These findings shed new light onto the molecular mechanisms of beta-cell glucose toxicity and apoptosis, demonstrate that TXNIP induction plays a critical role in this vicious cycle, and suggest that inhibition of TXNIP may represent a novel approach to reduce glucotoxic beta-cell loss.
Flow measurement by cardiovascular magnetic resonance: a multi-centre multi-vendor study of background phase offset errors that can compromise the accuracy of derived regurgitant or shunt flow measurements (2010)

In the absence of hardware or software upgrades able to reduce phase offset errors, all the systems tested appeared to require post-acquisition correction to achieve consistently reliable breath-hold measurements of flow. The effectiveness of offset correction software will still need testing with respect to clinical flow acquisitions.