gebhart/patrepeval huggingface dataset:
Peptide mapping of complex proteins at the low-picomole level with capillary electrophoretic separations (1992)

A variety of different peptide-mapping schemes are presented, with emphasis on the development of procedures which can be done with limited quantities (i.e. 5 pmol) of protein. Results are obtained from model proteins which contain disulfide bonds, which must be broken prior to fragmentation of the protein. A reaction involving the simultaneous use of tributylphosphine and 2-methylaziridine to reduce and alkylate the disulfide bonds is employed, due to favorable attributes of these reagents for the scaled-down procedure. The traditional performic acid oxidation reaction to cleave cystine groups is also successfully used with low-picomole quantities of protein. Three different protein digestion reagents are used: trypsin, chymotrypsin, and cyanogen bromide. Each reagent produces a unique mixture of peptides. Capillary electrophoresis is used to separate the peptides, offering high separation efficiencies, short analysis times, and compatibility with small sample sizes. In addition to the conventional use of UV detection for underivatized peptides, laser-induced fluorescence detection is employed in conjunction with an arginine-selective derivatization reaction. This latter procedure for derivatization and detection offers an alternative peptide-mapping mode, in which only the arginine-containing peptides are detected, and is useful in simplifying the peptide maps of large proteins.
Enteroendocrine cells: a site of ‘taste’ in gastrointestinal chemosensing (2008)

Taste-signaling molecules expressed in the gastrointestinal mucosa might participate in the functional detection of nutrients and harmful substances in the lumen and prepare the gut to absorb them or initiate a protective response. They might also participate in the control of food intake through the activation of gut-brain neural pathways. These findings provide a new dimension to unraveling the regulatory circuits initiated by luminal contents of the gastrointestinal tract.
MEASUREMENT OF CURVED OBJECTS USING GRADIENT BASED FITTING AND CSG MODELS (2000)

Since curved objects often contain no points that can be identified in overlapping images, it is difficult to apply a photogrammetric technique based on point identification. However, curved objects can very well be represented using 3D parameterized CSG (Constructive Solid Geometry) models. In this approach the shape and the pose of a curved object are given by the parameters of its CSG model. An initial measurement of the pose and the shape of a curved object can be obtained by the selection of a CSG model from a database and the approximate alignment of the object with the projections of the CSG model in a collection of images. These images are taken with a calibrated camera of which the interior and exterior orientations are known. In this paper we focus on the precise measurement of the pose and the shape of the curved object by fitting the projections of the CSG model precisely onto the edges of the curved object in the images. Observation equations are formulated relating the adaptation of the pose and shape parameters to the distance between pixels with high gray value gradients and edges of the projected CSG model. The model is fitted to the object in the images by estimating the changes to the pose and shape parameters by the least squares solution of these observation equations. The efficiency of the fitting algorithm is significantly improved by (a) the computation of a limited number of gradient strengths in a buffer around the projected model edges and (b) the elimination of gradients that are not perpendicular to the projected model edges. The practical application of the fitting algorithm is illustrated for images containing straight and curved pipes. The convergence behaviour and the robustness of the fitting algorithm are evaluated.
Two to three years of failure-free testing of a rechargeable pacemaker in experimental complete heart block. (1976)

Six hermetically sealed single cell rechargeable mercury-zinc pacemakers (B.T.) that will run continuously for over 4 years between rechargings have paced dogs with complete heart block for from 2 to 3 years. To maintain full cell capacity (over 1,000 mA hours) requires recharging for from 2-3 min/day to 60 to 80 hr once every 4 years, with any variation between these extremes being acceptable. Six realtime bench tests continue after over 7 years and accelerated tests have simulated a minimum of 50 years continuous pacing. Battery voltage is assessed by direct telemetry, eliminating the risk of patient intrinsic rhythm-pacemaker competition which is present with all current indirect (stimulation rate change) battery assessment techniques. The B.T. is an excellent 4-5 year primary pacemaker, fully rechargeable after several 4 year periods of battery rundown. A clinical test series has been initiated.
An Overview of Topical Antibiotics for Acne Treatment (1998)

Topical use of antibiotics is currently a widely accepted effective and safe treatment for acne. A review of the articles published in the past 30 years revealed that topical application of antibiotics such as erythromycin, clindamycin or tetracycline showed clinical effectiveness for mild to moderate inflammatory acne, especially when they are combined with zinc, tretinoin or benzoyl peroxide, while they showed little influence on noninflammatory acne. The main mechanism of action of topical antibiotics for acne treatment is inhibition of inflammation caused by bacteria rather than a direct bactericidal effect. The adverse reactions of topical antibiotics are mostly minor and negligible, while special attention should be given to the risk of development of resistant strains of Propionibacterium acnes. The development of new antibiotics is promising and will provide a wider range of therapeutic options for refractory cases.
Inulin as a Carrier for Contrast Agents in Magnetic Resonance Imaging (2001)

Magnetic resonance angiography (MRA) has put forth an impetus for the development of macromolecular GdIII complexes that have a prolonged lifetime in the vascular system. Herein, we report the synthesis and GdIII complexation of a new sugar conjugate based on inulin and the DO3A ligand (DO3A = 1,4,7,10-tetraazacyclododecan-1,4,7-triacetic acid). Two API-DO3ASQ conjugates (API = O-(aminopropyl)inulin, SQ = squaric acid = 3,4-dihydroxy-3-cyclobutene-1,2-dione) with different degrees of substitution (ds = 0.7 and ds = 1.5) were prepared from API by using the diethyl ester of squaric acid as a linking agent for the DO3A chelate. The efficacies of the resulting GdIII compounds were evaluated by investigation of their water 1H longitudinal-relaxation-rate enhancements at variable field (NMRD). A dramatic increase in relaxivity was observed in the more highly substituted conjugate (ds = 1.5); this prompted us to do a variable-temperature (17)O study in order to further characterize the relaxation parameters involved in this system. [Gd(API-DO3ASQ)] shows promising properties for application as a contrast agent for MRI.
Novel Delivery of SN38 Markedly Inhibits Tumor Growth in Xenografts, Including a Camptothecin-11–Refractory Model (2008)

Preclinical data suggest that EZN-2208 may be a promising anticancer agent in a wide variety of clinical settings, including tumors refractory to CPT-11 treatment.
A randomized controlled trial comparing lactulose, probiotics, and L-ornithine L-aspartate in treatment of minimal hepatic encephalopathy (2011)

Lactulose, probiotics, and LOLA significantly improve MHE and HRQoL in patients with chronic liver disease.
Signal waveform estimation in sensor array processing (1989)

The problem of estimating the waveform of narrowband signals impinging on a sensor array is of importance in many engineering applications. A known look-direction for a signal-of-interest (SOI) is usually assumed and a particular performance measure is optimized. To achieve optimality, these methods require the SOI to be uncorrelated with the other signals as well as the additive noise. Herein, the signal parameters (directions) are assumed to be unknown and an optimal estimator of all the signal waveforms is derived. The signal waveforms can be correlated or even coherent. The methods is based on a stochastic signal model and uses optimal signal parameter estimes obtained from the measurements via maximum likelihood estimation or weighted subspace fitting. Based on these estimates, the emitter signal covariance is estimated and a structured maximum a posteriori estimate of the signal waveforms is obtained. Simulations are presented comparing this estimator to the deterministic maximum likelihood estimator and the unstructured stochastic estimator.
Fiber-Optic Enzyme Biosensor for Direct Determination of Organophosphate Nerve Agents (1999)

A fiber-optic enzyme biosensor for the direct measurement of organophosphate nerve agents was developed. The basic element of this biosensor is organophosphorus hydrolase immobilized on a nylon membrane and attached to the common end of a bifurcated optical fiber bundle. The enzyme catalyzes the hydrolysis of organophosphate compounds to form stoichiometric amounts of chromophoric products that absorb light at specific wavelengths. The back-scattered radiation of the specific incident radiation was measured using a photomultiplier detector and correlated to the organophosphate concentration. The effects of buffer pH, temperature, and the units of enzyme immobilized on the steady-state and kinetic response of the biosensor were investigated to optimize the operating conditions for the fiber-optic enzyme biosensor. These conditions were then used to measure parathion, paraoxon, and coumaphos selectively without interference from carbamates and triazines. Concentrations as low as 2 microM can be measured in less than 2 min using the kinetic response. When stored in buffer at 4 degreesC the biosensor shows long-term stability.
Evaluating the benefits of an extended memory hierarchy for parallel streamline algorithms (2011)

The increasing cost of achieving sufficient I/O bandwidth for high end supercomputers is leading to architectural evolutions in the I/O subsystem space. Currently popular designs create a staging area on each compute node for data output via solid state drives (SSDs), local hard drives, or both. In this paper, we investigate whether these extensions to the memory hierarchy, primarily intended for computer simulations that produce data, can also benefit visualization and analysis programs that consume data. Some algorithms, such as those that read the data only once and store the data in primary memory, can not draw obvious benefit from the presence of a deeper memory hierarchy. However, algorithms that read data repeatedly from disk are excellent candidates, since the repeated reads can be accelerated by caching the first read of a block on the new resources (i.e. SSDs or hard drives). We study such an algorithm, streamline computation, and quantify the benefits it can derive.
Use of the University of California Los Angeles Integrated Staging System to Predict Survival in Renal Cell Carcinoma: An International Multicenter Study (2004)

This study defines the general applicability of the UISS for predicting survival in patients with RCC. The UISS is an accurate predictor of survival for patients with localized RCC applicable to external databases. Although the UISS may be useful for patients with metastatic RCC, it may be less accurate in this subset of patients due to the heterogeneity of patients and treatments.
April and Baff Connect Autoimmunity and Cancer (2000)

Recent studies of two newcomers to the TNF cytokine family have revealed an unsuspected link between autoimmunity and cancer. In this issue, Rennert et. al. ([1][1]) define the specific receptors for the orphan ligand known as APRIL (a proliferation-inducing ligand), which was previously recognized
Asymmetric Epoxidation of trans ‐β‐Methylstyrene and 1‐Phenylcyclohexene Using a D ‐Fructose‐Derived Ketone: ( R , R )‐ trans ‐β‐Methylstyrene Oxide and ( R , R )‐1‐Phenylcyclohexene Oxide (2003)

Abstract (R,R) trans‐beta‐Methylstyrene oxide Trans‐Methylstyrene Dimethoxymethane 1,2:4,5‐Di‐ O‐ isopropylidene‐D‐erythro‐2, 3‐hexodiulo‐2,6‐pyranose Tetrabutylammonium hydrogen sulfate Disodium ethylenediaminetetraacetate 1‐Phenylcyclohexene oxide Oxone
The E3 ligase PARC mediates the degradation of cytosolic cytochrome c to promote survival in neurons and cancer cells (2014)

The ability to withstand mitochondrial damage is especially critical for the survival of postmitotic cells, such as neurons. Likewise, cancer cells can also survive mitochondrial stress. We found that cytochrome c (Cyt c), which induces apoptosis upon its release from damaged mitochondria, is targeted for proteasome-mediated degradation in mouse neurons, cardiomyocytes, and myotubes and in human glioma and neuroblastoma cells, but not in proliferating human fibroblasts. In mouse neurons, apoptotic protease-activating factor 1 (Apaf-1) prevented the proteasome-dependent degradation of Cyt c in response to induced mitochondrial stress. An RNA interference screen in U-87 MG glioma cells identified p53-associated Parkin-like cytoplasmic protein (PARC, also known as CUL9) as an E3 ligase that targets Cyt c for degradation. The abundance of PARC positively correlated with differentiation in mouse neurons, and overexpression of PARC reduced the abundance of mitochondrially-released cytosolic Cyt c in various cancer cell lines and in mouse embryonic fibroblasts. Conversely, neurons from Parc-deficient mice had increased sensitivity to mitochondrial damage, and neuroblastoma or glioma cells in which PARC or ubiquitin was knocked down had increased abundance of mitochondrially-released cytosolic Cyt c and decreased viability in response to stress. These findings suggest that PARC-mediated ubiquitination and degradation of Cyt c is a strategy engaged by both neurons and cancer cells to prevent apoptosis during conditions of mitochondrial stress.
The hemoglobin of Urechis caupo. The cDNA-derived amino acid sequence. (1986)

The nucleotide sequence of a cDNA transcript containing part of the 5' noncoding region, the entire coding region, and the entire 3' noncoding region has been determined. The protein sequence predicted from the coding region matches almost exactly the aminoterminal sequence and the sequence of several peptides from Urechis caupo F-I globin. Only 11-20% of the amino acid positions are identical with those of other known globins.
Role of the LFA-1 molecule in cellular interactions required for antibody production in humans. (1986)

The lymphocyte function-associated antigen 1 (LFA-1) has been shown to play a role in various T cell functions in mice and humans including cytotoxicity, and proliferation to allogeneic cells and foreign antigens. These functions have been defined with specific monoclonal antibodies and were additionally confirmed by the investigation of patients with inherited deficiency in membrane LFA-1 expression. In this paper, we report our studies on the potential role of the LFA-1 molecule in T lymphocyte-dependent antibody responses. In a patient with a complete lack of membrane expression of LFA-1, there was no in vivo antibody response to vaccinal antigens such as tetanus, diphtheria toxoids, and polio virus, and no in vivo or in vitro antibody production to influenza virus, whereas serum immunoglobulin levels and antibodies to polysaccharides (isohemagglutinins, antibody to mannan, and a polysaccharide from Candida albicans) were detected in correlation with in vitro production of anti-mannan antibody. The defective antibody response to polypeptides was not secondary to poor antigen-specific T proliferation, because the latter was found to be present. Similarly, in vitro antibody production to influenza virus of normal cells was blocked by several anti LFA-1 monoclonal antibodies specific for the alpha subunit of the molecule, if they were added from the beginning of the culture. The antibody production blockade could be achieved with monoclonal antibody concentrations that partially preserved T cell proliferation. The helper effect of an influenza virus-specific helper T cell clone was also blocked. The targets of the blockade were shown by incubation experiments to be T cells and monocytes. In contrast, anti-LFA-1 monoclonal antibodies had no effect on pokeweed mitogen-induced B cell maturation into immunoglobulin-containing cells and on the anti-mannan antibody production. These combined data demonstrate that the LFA-1 molecule plays a role in T cell dependent antibody production to polypeptidic antigens but not in the antibody response to polysaccharides, although the antibody response to mannan is T cell dependent. It is proposed that the LFA-1 molecule is required to some extent for a antigen-presenting cells-T lymphocyte interaction and for the maintenance of a close association between antigen-specific helper T cells and small resting B lymphocytes. Polysaccharidic antigens that exhibit repetitive antigenic determinants might cross-link membrane immunoglobulins on B lymphocytes, thus allowing B cells to pass through a first step of activation requiring cognate T-B cell interaction.
Study on optimize cultivation technique mathematics model of naked barley in Diqing. (2007)

In the experiment,a regression analysis method with three unknown factors was adopted to study the naked barley yield's relation.The results showed the naked barley yield would attain above 2780 kg/hm2 when the seedling is about between 84.33×104/hm2 to 104.31×104/hm2,the nitrongen is applied about between 183.38 kg/hm2 to 204.51 kg/hm2,the P2O5 is applied about between 25.64 kg/hm2 to 27.69 kg/hm2 in Diqinq.
Spectroscopic stimulated Raman scattering imaging of highly dynamic specimens through matrix completion (2017)

Spectroscopic stimulated Raman scattering (SRS) imaging generates chemical maps of intrinsic molecules, with no need for prior knowledge. Despite great advances in instrumentation, the acquisition speed for a spectroscopic SRS image stack is fundamentally bounded by the pixel integration time. In this work, we report three-dimensional sparsely sampled spectroscopic SRS imaging that measures ~20% of pixels throughout the stack. In conjunction with related work in low-rank matrix completion (e.g., the Netflix Prize), we develop a regularized non-negative matrix factorization algorithm to decompose the sub-sampled image stack into spectral signatures and concentration maps. This design enables an acquisition speed of 0.8 s per image stack, with 50 frames in the spectral domain and 40,000 pixels in the spatial domain, which is faster than the conventional raster laser-scanning scheme by one order of magnitude. Such speed allows real-time metabolic imaging of living fungi suspended in a growth medium while effectively maintaining the spatial and spectral resolutions. This work is expected to promote broad application of matrix completion in spectroscopic laser-scanning imaging.
Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells (2004)

RNA interference (RNAi) mediated by short interfering RNAs (siRNAs) is a widely used method to analyze gene function. To use RNAi knockdown accurately to infer gene function, it is essential to determine the specificity of siRNA-mediated RNAi. We have assessed the specificity of 10 different siRNAs corresponding to the MEN1 gene by examining the expression of two additional genes, TP53 (p53) and CDKN1A (p21), which are considered functionally unrelated to menin but are sensitive markers of cell state. MEN1 RNA and corresponding protein levels were all reduced after siRNA transfection of HeLa cells, although the degree of inhibition mediated by individual siRNAs varied. Unexpectedly, we observed dramatic and significant changes in protein levels of p53 and p21 that were unrelated to silencing of the target gene. The modulations in p53 and p21 levels were not abolished on titration of the siRNAs, and similar results were obtained in three other cell lines; in none of the cell lines tested did we see an effect on the protein levels of actin. These data suggest that siRNAs can induce nonspecific effects on protein levels that are siRNA sequence dependent but that these effects may be difficult to detect until genes central to a pivotal cellular response, such as p53 and p21, are studied. We find no evidence that activation of the double-stranded RNA-triggered IFN-associated antiviral pathways accounts for these effects, but we speculate that partial complementary sequence matches to off-target genes may result in a micro-RNA-like inhibition of translation.
Palliative total skin electron beam therapy (TSEBT) for advanced cutaneous T-cell lymphoma. (2008)

Our aim was to analyze the effectiveness of palliative total skin electron beam therapy (TSEBT) in the management of advanced cutaneous T-cell non-Hodgkin's lymphoma (CTCL). Eighteen patients (median age 59 years) with advanced and therapy-refractory CTCL in stages IIB-IV were treated with TSEBT for the first time. The most common histological subtype was Mycosis fungoides (72%). All patients suffered from lymphoma-associated symptoms. Median daily fractions of 1 Gy were administered up to a median total dose of 25 Gy. The median follow-up period was 11 months. Nine patients (50%) achieved a complete response and seven patients (39%) had a limited response. The actuarial one-year progression-free survival was 24%. Four patients (22%) had continuing remission over a median period of six months. Lymphoma associated symptoms were improved in 16 patients (89%). The median overall survival after receiving TSEBT was 12 months, resulting in an actuarial one-year overall survival of 48%. Treatment related acute effects (grade 1 or 2) were observed in all patients during radiation therapy. Transient grade 3 epitheliolyses developed in five patients (28%), late skin effects (grade 1 and 2) in 16 patients (89%), and hypohidrosis was seen in six patients (33%). We conclude that TSEBT is a very efficient and tolerable palliative treatment for patients with advanced CTCL.
DNA Plasmid Vaccination for Multiple Sclerosis (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Neurology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Neurology journal
Photoplethysmographic imaging of high spatial resolution (2011)

We present a new method of formation photoplethysmographic images with high spatial resolution from video recordings of a living body in the reflection geometry. The method (patent pending) is based on lock-in amplification of every pixel of the recorded video frames. A reference function required for synchronous detection of cardiovascular pulse waves is formed from the same frames. The method is featured by ability to visualize dynamic changes in cardiovascular pulse wave during the cardiac (or respiratory) cycle. We demonstrate that the system is capable to detect the minimal irritations of the body such as gentle scratching of the skin by own finger.