gebhart/patrepeval huggingface dataset:
Immunological Comparison of Antibodies to Porcine Zonae Pellucidae in Rats and Rabbits (1984)

Rats and rabbits immunized with porcine zonae pellucidae (ZP) develop antibodies to specific ZP proteins. The proteins recognized by rabbit and rat antibodies were identified using immunoblot procedures. For these studies, proteins were separated by high-resolution, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Proteins were transferred electrophoretically to nitrocellulose paper and antibody binding determined using 125I-labeled Staphylococcus aureus protein A (Staph A). The three major porcine ZP glycoprotein families were recognized by antibodies in the sera of both rabbits and rats. These antibodies were further characterized using Staph A binding and enzyme-linked (ELISA) immunoassays. Rabbits immunized with porcine ZP developed antibodies which recognized the porcine ZP as well as rat ZP antigens. Rats immunized with porcine ZP produced antibodies which recognized porcine ZP antigens but did not react with rat ZP antigens. While antibodies to porcine ZP, detected by Staph A binding and ELISA assays, correlated with infertility in rabbits [Wood et al. (1981) Biol. Reprod. 25:439], rats having these antibodies could have normal offspring. Continued fertility in rats following immunization with porcine ZP may be due to the failure to induce autoantibodies which recognized self ZP antigenic determinants.
Transient adhesion of neutrophils to endothelium. (1989)

Fluorescently labeled polymorphonuclear leukocytes (PMN) were used to measure adhesion to human umbilical vein endothelial cells (EC) cultured in vitro. Stimulation of PMN with phorbol dibutyrate (PDB), TNF, or C5a caused an increase in adhesion followed by a return to prestimulation levels of adhesion of longer times of incubation. Maximal adhesion of PMN to EC occurred rapidly in response to C5a (5 min) and more slowly with TNF or PDB (15 min). PMN stimulated to adhere with C5a detached from EC by 15 min. PMN from CD11/CD18-deficient patients and PMN incubated with anti-CD18 mAbs failed to bind to EC despite maximal stimulation. Anti-CD11a/CD18 and anti-CD11b/CD18 each partially inhibited adhesion, and a combination of these two reagents completely blocked adhesion. The adhesion we measured was therefore completely dependent on CD11/CD18, and CD11a/CD18 and CD11b/CD18 each contributed to adhesion. Stimuli that enhanced adhesion of PMN to EC also enhanced expression of CD11b/CD18 on the cell surface, but the time course of expression correlated poorly with changes in adhesivity. To determine if changes in the expression of CD11b/CD18 are necessary for the changes in adhesivity, we used enucleate cytoplasts that did not increase expression of CD11b/CD18. Cytoplasts showed a normal rise and fall in adhesivity in response to PDB. We conclude that the transient adhesion of stimulated PMN to naive EC is regulated by changes in the nature of existing CD11/CD18 molecules on the PMN surface. Changes in expression of CD11b/CD18 may contribute to enhancement of adhesivity, but a definite role for this phenomenon has yet to be established.
Fabrication of Local Ge-on-Insulator Structures by Lateral Liquid-Phase Epitaxy: Effect of Controlling Interface Energy between Ge and Insulators on Lateral Epitaxial Growth (2009)

Local Ge-on-insulator (GOI) structures were fabricated by means of lateral liquid-phase epitaxy (LPE). We investigated both effects of microcrucible size and underlying insulator materials that determine the interface energy with liquid Ge. We demonstrated that choosing narrow crucibles and controlling interface energy suppresses undesirable Ge aggregation and random nucleation during LPE growth. By utilizing alternative La2O3 high-k dielectrics instead of conventional SiO2 insulators, single-crystal 44-µm-long Ge wires were fabricated within 1-µm-wide microcrucibles. The improved mechanism is also discussed in terms of interface energy and random nucleation during LPE growth.
Efficient synthesis of (−)- and (+)-tricyclic compounds with enone functionalities in rings A and C. A novel class of orally active anti-inflammatory and cancer chemopreventive agents (2003)

Novel tricyclic compounds with enone functionalities in rings A and C [tricyclic-bis-enone (TBE) compounds] were designed on the basis of the structure of a synthetic triterpenoid, 2-cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid (CDDO)(1), which is a promising drug candidate for prevention and/or treatment of cancer and inflammatory diseases whose pathogenesis may involve excessive production of nitric oxide (NO) and/or prostaglandins. A series of TBE compounds in racemic form shows high inhibitory activity against production of NO induced by interferon-[gamma](IFN-[gamma]) in mouse macrophages. One of these compounds, (+/-)-(4a[small beta],8a[small beta],10a[small alpha])-1,2,4a,6,8a,9,10,10a-octahydro-1,1,4a,8a-tetramethyl-2,6-dioxophenanthrene-3,7-dicarbonitrile ((+/-)-3), is orally active at 15 mg kg(-1)(single administration) in a preliminary study using mouse peritoneal inflammation induced by thioglycollate and IFN-[gamma]. Therefore, we desired to synthesize optically active TBE compounds for a comparison of the biological potency of both enantiomers. We now describe the synthesis of both enantiomers of (4a[small beta],8a[small beta],10a[small alpha])-1,2,4a,6,8a,9,10,10a-octahydro-1,1,4a,8a-tetramethyl-2,6-dioxophenanthrene-3-carbonitrile (2) and 3 from commercially available simple compounds. Interestingly, (+)-3 having the same configuration as the CDDO antipode shows about 10 times higher inhibitory activity than (-)-3 on NO production in mouse macrophages. In contrast, (-)-3 inhibits proliferation of MCF-7 breast cancer cells, whereas (+)-3 does not.
Synthesis of Ring B-Rearranged Taxane Analogs (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Ring B-Rearranged Taxane AnalogsLarry L. Klein, Clarence J. Maring, Leping Li, Clinton M. Yeung, Sheela A. Thomas, David J. Grampovnik, J. J. Plattner, and Rodger F. HenryCite this: J. Org. Chem. 1994, 59, 9, 2370–2373Publication Date (Print):May 1, 1994Publication History Published online1 May 2002Published inissue 1 May 1994https://pubs.acs.org/doi/10.1021/jo00088a017https://doi.org/10.1021/jo00088a017research-articleACS PublicationsRequest reuse permissionsArticle Views144Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Tunnel agents for enhanced Internet QoS (1998)

The authors present an agent-based approach for improved quality of service provisioning which follows the open programmable networks paradigm for complementing still-defective Internet reservation schemes. It provides more complete QoS provisioning in a flexible, highly scalable manner. The authors' Java-based agent platform might work especially well in heterogeneous environments, which distributed multimedia systems are most likely to face.
A homing mechanism for bone marrow-derived progenitor cell recruitment to the neovasculature (2006)

CD34+ bone marrow-derived progenitor cells contribute to tissue repair by differentiating into endothelial cells, vascular smooth muscle cells, hematopoietic cells, and possibly other cell types. However, the mechanisms by which circulating progenitor cells home to remodeling tissues remain unclear. Here we show that integrin alpha4beta1 (VLA-4) promotes the homing of circulating progenitor cells to the alpha4beta1 ligands VCAM and cellular fibronectin, which are expressed on actively remodeling neovasculature. Progenitor cells, which express integrin alpha4beta1, homed to sites of active tumor neovascularization but not to normal nonimmune tissues. Antagonists of integrin alpha4beta1, but not other integrins, blocked the adhesion of these cells to endothelia in vitro and in vivo as well as their homing to neovasculature and outgrowth into differentiated cell types. These studies describe an adhesion event that facilitates the homing of progenitor cells to the neovasculature.
Rapid presumptive identification of medically relevant yeasts to the species level by polymerase chain reaction and restriction enzyme analysis (1994)

A method for the rapid presumptive differentiation of a panel of 12 clinically relevant yeasts to the species level was developed on the basis of evaluation by the polymerase chain reaction (PCR) of the gene coding for the small ribosomal subunit 18S-rRNA. The method involved restriction enzyme analysis of PCR products obtained with primers common to all fungi. Using six restriction enzymes, AluI, BanI, BbsI, DraII, Eco147I and NheI, characteristic PCR-restriction enzyme patterns were obtained for Candida albicans, Candida tropicalis, Candida krusei, Candida kefyr, Candida lusitaniae, Candida guilliermondii, Candida glabrata and Saccharomyces cerevisiae, as well as for the pairs Candida parapsilosis/Candida viswanathii and Trichosporon beigelii/Cryptococcus neoformans. The procedure does not involve hybridization steps or the use of radioactivity and can be completed within one working day.
Polyglutamine aggregates alter protein folding homeostasis in Caenorhabditis elegans (2000)

Expansion of polyglutamine repeats in several unrelated proteins causes neurodegenerative diseases with distinct but related pathologies. To provide a model system for investigating common pathogenic features, we have examined the behavior of polyglutamine expansions expressed in Caenorhabditis elegans. The expression of polyglutamine repeats as green fluorescent protein (GFP)-fusion proteins in body wall muscle cells causes discrete cytoplasmic aggregates that appear early in embryogenesis and correlates with a delay in larval to adult development. The heat shock response is activated idiosyncratically in individual cells in a polyglutamine length-dependent fashion. The toxic effect of polyglutamine expression and the formation of aggregates can be reversed by coexpression of the yeast chaperone Hsp104. The altered homeostasis associated with polyglutamine aggregates causes both the sequestration of an otherwise soluble protein with shorter arrays of glutamine repeats and the relocalization of a nuclear glutamine-rich protein. These observations of induced aggregation and relocalization have implications for disorders involving protein aggregation.
An Outbreak of Pyrogenic Reactions in Chronic Hemodialysis Patients Associated with Hemodialyzer Reuse (1995)

In February 1992, 22 patients undergoing chronic hemodialysis at an outpatient dialysis center experienced pyrogenic reactions (PR). The PR rate was significantly greater (p < 0.001) during the epidemic (February 3-5) than the pre-epidemic period (November 1, 1992-February 1, 1992). All patients with PR used dialyzers that had been manually reprocessed either on February 1 or 3. These dialyzers contained up to 120.8 EU/ml of endotoxin in the blood compartment. The only dialyzer reprocessed before February 1 that was available for analysis was found to contain no detectable endotoxin, while dialyzers reprocessed during the epidemic period contained a median endotoxin concentration of 52.8 EU/ml. The bioburden of water used to prepare dialysate was in excess of the Association for the Advancement of Medical Instrumentation (AAMI) standard for water, < or = 200 colony forming units (CFU)/ml. Samples of treated water collected in the reuse area were within AAMI standards at the time of the investigation (February 11 and February 26), but before the investigation, water samples were assayed with a culture method that could not detect microbial concentrations below 10(3) CFU/ml. In addition, the treated water feed line to the disinfectant container may never have been disinfected. However, no samples were collected from this line during the investigation. This outbreak emphasizes the need to use water that meets the AAMI bacteriologic and endotoxin standards of < or = to 200 CFU/ml and/or 5 EU/ml, respectively, for reprocessing hemodialyzers nad to ensure that appropriate culture techniques are used for treated water dialysate.
Sequence of human protein kinase C α (1990)

Journal Article Sequence of human protein kinase C α Get access Günter Finkenzeller, Günter Finkenzeller University of Freiburg, Institute of Molecular Cell Biologyc/o Gödecke AG, D-7800 Freiburg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Dieter Marmé, Dieter Marmé University of Freiburg, Institute of Molecular Cell Biologyc/o Gödecke AG, D-7800 Freiburg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Hubert Hug Hubert Hug University of Freiburg, Institute of Molecular Cell Biologyc/o Gödecke AG, D-7800 Freiburg, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 8, 25 April 1990, Page 2183, https://doi.org/10.1093/nar/18.8.2183 Published: 25 April 1990 Article history Received: 13 March 1990 Published: 25 April 1990
Fabrication of semipermeable hollow fiber membranes with highly aligned texture for nerve guidance (2005)

In order to improve the guidance potential of a nerve entubulation bridging device, highly aligned textures were formed on the inner surface of semipermeable hollow fiber membranes (HFMs) during the wet phase inversion process. By precisely controlling the fabrication parameters, such as polymer solution flow rate, coagulant solution flow rate, and the air-gap distance, also called drop height, different-sized aligned grooves can be fabricated on the inner surface of HFMs. Preliminary studies using in vitro dorsal root ganglion (DRG) regeneration assay showed that both the alignment and outgrowth rate of regenerating axons increased significantly on HFMs with aligned textures compared to those on HFMs with a smooth inner surface. Studies in progress are evaluating axonal outgrowth and regeneration using in vivo sciatic-nerve and spinal-cord-injury models.
The Classic (1985)

Duncan Clark McKeever (Fig. I) was born on September 13, 1905, in Valley Falls, Kansas. After attending local schools, he graduated from the University of Kansas Medical School in 1929. As a naval reservist, he spent the next four years in naval training centers, followed by a residency in pathology at St. Luke's Hospital in Kansas City. While there, he fell under the influence of Drs. Frank Dickson and Rex Divley and became interested in orthopedics. After three years of association with them, he moved to Houston in 1939 to open a private practice. From 1941 to 1945, during World War II, he was back in the navy as chief of several hospitals. After the war, he returned to his private practice. McKeever's knowledge of engineering principles led to his research interest in stress analysis as it applied to operative procedures on bones. His advanced ideas in orthopedic surgery led him to develop original procedures, and his exacting attention to details helped make them successful. His success led to additional innovative procedures, which included prostheses of the hip, patella, and tibial plateau. His continuing studies kept him in demand as a teacher. Frequent visits from his many friends included those from Latin American countries. Dr. McKeever enjoyed hunting and fishing, and he was always delighted to be at his ranch. McKeever was one of the founders of the Association of Bone and Joint Surgeons and became its third president. He was also a member and active participant in many orthopedic organizations and on local hospital boards and staffs. On a rainy evening, October 13, 1959, when driving someone else's car, he ran out of gas; while filling the tank, he was struck by another car and killed. His untimely death was a great loss to orthopedics as well as a personal loss to his many friends.
Direct Association of Adenosine Deaminase with a T Cell Activation Antigen, CD26 (1993)

CD26, the T cell activation molecule dipeptidyl peptidase IV (DPPIV), associates with a 43-kilodalton protein. Amino acid sequence analysis and immunoprecipitation studies demonstrated that this 43-kilodalton protein was adenosine deaminase (ADA). ADA was coexpressed with CD26 on the Jurkat T cell lines, and an in vitro binding assay showed that the binding was through the extracellular domain of CD26. ADA deficiency causes severe combined immunodeficiency disease (SCID) in humans. Thus, ADA and CD26 (DPPIV) interact on the T cell surface, and this interaction may provide a clue to the pathophysiology of SCID caused by ADA deficiency.
Distinct regulation of IL-8 and MCP-1 by LPS and interferon-&ggr;-treated human peritoneal macrophages (1998)

Our data show differences in LPS/IFN-gamma regulation for IL-8 and MCP-1 in both highly activated and in resting, mature peritoneal macrophages, suggesting distinct pathways for these chemokines that may offer a means of control for the specific recruitment of neutrophils and monocytes/macrophages in bacterial peritonitis.
A-ECMS: An Adaptive Algorithm for Hybrid Electric Vehicle Energy Management (2006)

Hybrid Electric Vehicles (HEV) improvements in fuel economy and emissions strongly depend on the energy management strategy. In this paper a new control strategy called Adaptive Equivalent Consumption Minimization Strategy (A-ECMS) is presented. This real-time energy management for HEV is obtained adding to the ECMS framework an on-the-fly algorithm for the estimation of the equivalence factor according to the driving conditions. The main idea is to periodically refresh the control parameter according to the current road load, so that the battery State of Charge (SOC) is maintained within the boundaries and the fuel consumption is minimized. The results obtained with A-ECMS show that the fuel economy that can be achieved is only slightly sub-optimal and the operations are charge-sustaining.
A dynamic threshold voltage MOSFET (DTMOS) for very low voltage operation (1994)

A new mode of operation for Silicon-On-Insulator (SOI) MOSFET is experimentally investigated. This mode gives rise to a Dynamic Threshold voltage MOSFET (DTMOS). DTMOS threshold voltage drops as gate voltage is raised, resulting in a much higher current drive than regular MOSFET at low V/sub dd/. On the other hand, V/sub t/ is high at V/sub gs/=0, thus the leakage current is low. Suitability of this device for ultra low voltage operation is demonstrated by ring oscillator performance down to V/sub dd/=0.5 V.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Differential expression of the mouse D2 dopamine receptor isoforms (1991)

We have identified and characterized the cDNAs corresponding to the mouse D2 dopamine receptors. We show that in the mouse the D2 dopamine receptor is found in two forms, generated by alternative splicing of the same gene, mRNA distribution analysis of areas expressing the D2 receptors shows that the larger form is the most abundant, except in the brain stem where the shorter form is predominant. Membranes of mammalian cells transiently transfected with both forms of D2 receptor bind [3H]spiperone with a high affinity.
An automated system for semantic object labeling with soft object recognition and dynamic programming segmentation (2015)

This paper presents an automated system for generating a semantic map of inventory in a retail environment. Developing this map involves assigning a department label to each discrete section of shelving. We use a priori information to boost data from laser and camera sensors for object recognition and semantic labeling. We introduce a soft object map and a dynamic programming algorithm for point cloud segmentation. The primary contribution of this work is the integration of multiple systems including an automated path planning and navigation subsystem and a semantic mapping object recognition system. This work also represents an important contribution to robots working reliably in human environments. To our knowledge this is the first actual implementation of a fully automated robot inventory labeling system for a retail environment. The framework presented in this paper is easily scalable to other retail environments and is also relevant in any indoor environment with organized shelves, such as business storage facilities and hospital pharmacies.
Virtual Babyz: Believable Agents with Narrative Intelligence (1999)

This paper describes the interactive narrative experiences in Babyz, an interactive entertainment product for the PC currently in development at PF Magic / Mindscape in San Francisco, to be released in October 1999. Babyz are believable agents designed and implemented in the tradition of Dogz and Catz, Your Virtual Petz. As virtual human characters, Babyz are more intelligent, expressive and communicative than their Petz predecessors, allowing for both broader and deeper narrative possibilities. Babyz are designed with behaviors to support entertaining short-term narrative experiences, as well as long-term emotional relationships and narratives.
Basolateral Sorting of Furin in MDCK Cells Requires a Phenylalanine-Isoleucine Motif Together with an Acidic Amino Acid Cluster (1999)

Furin is a subtilisin-related endoprotease which processes a wide range of bioactive proteins. Furin is concentrated in the trans-Golgi network (TGN), where proteolytic activation of many precursor proteins takes place. A significant fraction of furin, however, cycles among the TGN, the plasma membrane, and endosomes, indicating that the accumulation in the TGN reflects a dynamic localization process. The cytosolic domain of furin is necessary and sufficient for TGN localization, and two signals are responsible for retrieval of furin to the TGN. A tyrosine-based (YKGL) motif mediates internalization of furin from the cell surface into endosomes. An acidic cluster that is part of two casein kinase II phosphorylation sites (SDSEEDE) is then responsible for retrieval of furin from endosomes to the TGN. In addition, the acidic EEDE sequence also mediates endocytic activity. Here, we analyzed the sorting of furin in polarized epithelial cells. We show that furin is delivered to the basolateral surface of MDCK cells, from where a significant fraction of the protein can return to the TGN. A phenylalanine-isoleucine motif together with the acidic EEDE cluster is required for basolateral sorting and constitutes a novel signal regulating intracellular traffic of furin.
Melanization of Cryptococcus neoformans in Murine Infection (1999)

Cryptococcus neoformans is a fungus that is pathogenic in humans and that can produce melanin in vitro. Melanization is associated with virulence, but there is no evidence that melanin is made during infection. Melanins are difficult to study because they are amorphous and insoluble. Melanin-binding peptides from a phage display library were used to demonstrate that C. neoformans makes melanin-like compounds in tissue. Melanin-binding peptides were characterized by a high proportion of positively charged and aromatic residues. Two other methods, demonstration of an antibody response to melanin in mice infected with C. neoformans and analysis of yeast cell walls in infected tissue by light microscopy, were used to support these findings. The demonstration that C. neoformans melanizes in tissue has important implications for pathogenesis and drug discovery.