gebhart/patrepeval huggingface dataset:
Recombinant human insulin-like growth factor I stimulates growth and has distinct effects on organ size in hypophysectomized rats. (1988)

Recombinant human insulin-like growth factor I (rhIGF-I) was infused subcutaneously into hypophysectomized rats for as long as 18 days. Three hundred micrograms (39 nmol) of rhIGF-I per day and 200 milliunits (4.5 nmol) of human growth hormone (hGH) per day increased body weight, tibial epiphyseal width, longitudinal bone growth, and trabecular bone formation similarly. Weight gains of the kidneys and spleen, however, were greater with rhIGF-I than with hGH, whereas the weight of the epididymal fat pads was reduced with rhIGF-I. The weight of the thymus was increased by rhIGF-I treatment. Thus, IGF-I administered over a prolonged period of time mimics GH effects in hypophysectomized rats. Quantitative differences between rhIGF-I and hGH treatment with respect to organ weights may be related to different forms of circulating IGF-I or may be due to independent effects of GH and IGF-I. The results support the somatomedin hypothesis, but they also stress the role of GH as a modulator of IGF-I action.
RNA Interference from Multimeric shRNAs Generated by Rolling Circle Transcription (2006)

Methods most commonly used for producing small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) are chemical synthesis and intracellular expression from engineered vectors. For shRNAs, chemical synthesis is very costly and construction of vectors is laborious. Synthesis by phage RNA polymerases from their natural promoters results in a 5 -terminal triphosphate that can trigger an interferon (IFN) response. Moreover, due to the requirement of phage promoters for 5 - GPuPuPu sequences for transcription initiation, shRNA transcripts may have extra 5 -nucleotides that can constrain the sequences that can be targeted. Also, the 3 ends may have an additional n + 1 nucleotide not encoded by the template. Here we present a novel approach for synthesizing functional shRNAs via rolling circle transcription (RCT) of small (approximately 70 nt) single-stranded DNA circles using T7 RNA polymerase, which avoids these issues. Due to internal pairing, these circles are dumbbell-shaped. RCT produces large transcripts (>10 kb in length) consisting of multimers (>150 copies) of shRNAs in the absence of promoter, terminator, or primer sequences. Dumbbells targeting red fluorescent protein (DsRed), human tumor necrosis factor-alpha (TNF-alpha) and hepatitis C virus (HCV) internal ribosome entry site (IRES) were prepared and transcribed. The resulting long transcripts are substrates for Dicer. When introduced into 293FT and Huh7 cells, the multimeric transcripts inhibited their target genes at levels similar to an equivalent mass of monomeric shRNAs, indicating that they can enter the RNAi pathway. Thus, rolling circle transcription of small DNA dumbbells provides a new source of biologically active interfering RNA.
Antimicrobial therapy of selected diseases in turkeys, laying hens, and minor poultry species in Canada. (2013)

This paper identifies common poultry diseases requiring antimicrobial therapy, antimicrobials deemed efficacious to treat these diseases, and antimicrobial resistance (AMR) in these commodity-pathogen combinations, and describes current residue issues and minor use minor species (MUMS) guidelines. Veterinarians with turkey/layer expertise and diagnosticians were surveyed to determine the bacterial and protozoal diseases diagnosed in the last 5 years. Avian pathogenic Escherichia coli, Staphylococcus aureus, and Ornithobacterium rhinotracheale were the 3 most frequently diagnosed pathogens of turkeys. In layers, E. coli-peritonitis, and Clostridium perfringens/Eimeria spp. infections were the most common diagnoses. A literature review identified 32 antimicrobials as efficacious and/or recommended for treating these diseases. Surveillance and monitoring indicate the presence of enteric resistant organisms from some of these avian species (including resistance to antimicrobials of very high importance to human medicine). This paper highlights the need for surveillance of pathogen frequency, antimicrobial use (AMU), and AMR particularly in turkeys.
The Use Of A Simplified Model For Particulate Scatter (1990)

A simplified model of scatter from particulate-coated optical surfaces has been presented formerly. Results using it are compared with measured data and with a more rigorous model that uses integrated Mie and imaging techniques.
Bone Marrow Transplantation: A Review (1993)

The indications for bone marrow transplantation (BMT) continue to expand as supportive care improves and alternative stem cell sources have been exploited. The application of allogeneic BMT has expanded to include unrelated histocompatibility antigen-matched donors and partially matched family donors. While the results of these transplants are not as good as those with sibling donors, these alternative donors allow curative therapy to be delivered to patients with leukemia, aplastic anemia, and immunodeficiency diseases who otherwise would not be eligible for curative therapy. Autologous BMT has emerged as a curative therapy for patients with non-Hodgkin's lymphoma, Hodgkin's disease, acute myeloid leukemia, and acute lymphoblastic leukemia. In addition, dose-intensive therapy with marrow or peripheral blood stem cell support to patients with Stage II, III, and IV breast carcinoma is under intense study in single and multiple-institution studies. Important issues under active study are prophylaxis for graft-versus-host-disease, the role of marrow purging in autologous BMT, the use of cytokine and chemotherapy-mobilized peripheral blood stem cells, and control of infectious diseases. This review summarizes current results in both allogeneic and autologous bone marrow transplantation, issues in marrow graft manipulations, issues in infectious disease control, the application of gene therapy to correct genetic disease through bone marrow or peripheral blood infusion, and current concepts in post-BMT immunization.
Direct Syntheses of Ordered SBA-15 Mesoporous Silica Containing Sulfonic Acid Groups (2000)

A simple procedure has been developed for the syntheses of functionalized mesoporous materials with sulfonic groups involving the co-condensation of tetraethoxysilane and mercaptopropyltrimethoxysilane in the presence of block copolymers and hydrogen peroxide under acidic conditions. The modified SBA-15 materials show hexagonal mesoscopic order and pore sizes up to 60 Å, with acid exchange capacities ranging from 1 to 2 mequiv of H+/g of SiO2, surface areas up to 800 m2/g, and excellent thermal and hydrothermal stabilities. The formation of the sulfonic groups during co-condensation of the silica species coincides with enhanced mesoscopic ordering and changes in the adsorption properties of the final materials. 31P MAS NMR measurements of chemically adsorbed triethylphosphine oxide confirm the presence of Brönsted acid centers that are stronger that those found in Al-MCM-41. Finally, this procedure has been generalized to prepare functionalized mesoporous solids containing sulfonic groups and other organic moieties.
A Clinical Trial of Laser Thermal Angioplasty in Patients with Advanced Peripheral Vascular Disease (1990)

A 3-year prospective trial of laser thermal-assisted balloon angioplasty in 28 patients included 27 who had advanced peripheral vascular disease (severe tissue loss, gangrene, infection, and rest pain), 7 who were failures of previous therapy (surgery and thrombolysis), and 4 who were high risk for operation (myocardial infarction within 6 weeks and/or ejection fractions of less than or equal to 20%). Laser angioplasty was performed in the operating room via a groin incision by a surgeon-radiologist team. In the 27 patients with advanced peripheral vascular disease (ankle-brachial systolic pressure index [ABI] 0.27 +/- 0.2 in 10 nondiabetic, and 0.46 +/- 0.1 in 17 diabetic patients), recanalization of the native vessel was successful in 16, and patency was restored in 2 chronically occluded polytetrafluorethylene (PTFE) grafts. In these 18 (67%) successfully recanalized patients, however, five amputations were required within 1 month, and another six were needed between 8 and 12 months. Early amputations were caused by a failure of wound healing, even through angioplasty sites were patent. Late amputations were caused by reocclusion of the treated site in five of six patients. In the remaining seven patients in whom laser angioplasty alone was successful, five had healed limbs at 6 to 24 months and two remain incompletely healed but functional. The patency for successful procedures ranged from 48 hours to 25 months (5.6 +/- 6.4 mean months, +/- SD), with cumulative patency by life-table analysis of 55.5% at 3 months, 38.8% at 6 months, and 11.1% at 12 months. There were no procedure-related deaths. Complications included seven arterial wall perforations by the laser probe. We conclude that laser angioplasty has a limited role in advanced peripheral vascular disease but may provide an interval patency, thus allowing postponement of operation for high-risk patients until their medical conditions permits surgery, or to correct local tissue necrosis or infection in the operative field before reconstruction, and to restore patency to thrombosed PTFE grafts.
Long-cavity, multiple-phase-shift, distributed feedback laser for linewidth narrowing (1989)

We have developed the long-cavity multiple-phase-shift distributed feedback (MPS-DFB) laser diode with three phase shifts in the corrugation for linewidth narrowing. Stable single-mode operation up to 30 mW and narrow linewidth of 830 kHz were obtained for a 1.2 mm cavity MPS-DFB laser.
Sub-10 nm lithography with self-assembled monolayers (1996)

Dots demonstrating critical resist dimensions of approximately 5 to 6 nm were formed in an octadecylsiloxane monolayer on silicon by electron beam exposure using a digital scanning electron microscope at 20 keV beam energy. The patterned dots were observed by imaging with an atomic force microscope (AFM). The electron beam size was measured to confirm that it is not the limiting factor in the exposure resolution. The limit that prevents the observation of smaller structures is either the small contrast in the AFM imaging for smaller dots or an intrinsic material limit caused by the secondary electron range.
Self-Assembly of ABA Amphiphilic Triblock Copolymers into Vesicles in Dilute Solution (2005)

Self-assembly of an ABA amphiphilic triblock copolymer into vesicles in dilute solution was studied by successfully combining experimental methods and a real-space self-consistent field theory in three-dimensional space. It was found experimentally that vesicle size was sensitive to the initial copolymer concentration in the organic solvent. Also, the aggregate morphologies and vesicles sizes were found to be dependent on the annealing time. A number of complex vesicles, such as global, long-style, trigonal, and necklacelike vesicles, were obtained in our experiments. Moreover, the corresponding microstructures were produced in our simulations. The results show that various vesicles in dilute solution are formed solely on account of the inhomogeneous density distribution in the local region in nature. Our simulations confirm that the structural complexity coexisting behavior in the single-amphiphile systems is largely attributed to the metastability rather than the polydispersity of the triblock copolymer. These metastable states should strongly depend on the pathway of the system on the free energy landscapes, which is governed by the initial condition.
Effect of Brij-78 on Systemic Delivery of Insulin from an Ocular Device (1997)

An ocular insert is developed for the controlled systemic delivery of insulin. Commercially available Gelfoam absorbable gelatin sponge, USP, is used in the fabrication of the ocular insert in the form of a matrix system. Two eyedrop formulations and 13 eye device formulations were evaluated. The efficacy of insulin ocular delivery was quantitated by monitoring the changes in its pharmacological response (i.e., blood glucose lowering). The in vivo results from devices containing 0.5 or 1.0 mg of insulin with 20 micrograms of polyoxyethylene-20-stearyl ether (Brij-78) give a substantial improvement in insulin activity and a significant prolongation in its duration compared with the eyedrops. In addition, the mean blood glucose concentration returns to nearly normal levels within 60 min after the removal of the device. Overall, the application of the Gelfoam device makes it feasible to obtain a prolonged systemic delivery of insulin within the desired therapeutic levels without the risk of hypoglycemia.
Design of a SIP-based Real-time Visitor Conversation and Door Control Architecture using a Home Gateway (2006)

Though typical video door phone systems can be used to talk to the visitors at the door, they have some shortcomings: the resident should stay in house and he/she is not informed of any visitor information on his/her return to home. To overcome these limitations, we propose an architecture supporting user mobility, with which the resident at anywhere can view and converse with a visitor at the front door using PCs, PDA's and home servers with Internet connections. We implemented the proposed architecture using SIP (session initiation protocol) and a home gateway system connected to a conventional intercom or a video door phone system.
Fabrication of Releasable Single‐Crystal Silicon–Metal Oxide Field‐Effect Devices and Their Deterministic Assembly on Foreign Substrates (2011)

Abstract A new class of thin, releasable single‐crystal silicon semiconductor device is presented that enables integration of high‐performance electronics on nearly any type of substrate. Fully formed metal oxide–semiconductor field–effect transistors with thermally grown gate oxides and integrated circuits constructed with them demonstrate the ideas in devices mounted on substrates ranging from flexible sheets of plastic, to plates of glass and pieces of aluminum foil. Systematic study of the electrical properties indicates field‐effect mobilities of ≈710 cm 2 V −1 s −1 , subthreshold slopes of less than 0.2 V decade −1 and minimal hysteresis, all with little to no dependence on the properties of the substrate due to bottom silicon surfaces that are passivated with thermal oxide. The schemes reported here require only interconnect metallization to be performed on the final device substrate, which thereby minimizes the need for any specialized processing technology, with important consequences in large‐area electronics for display systems, flexible/stretchable electronics, or other non‐wafer‐based devices.
Mechanisms of transdermal drug delivery (1997)

Visualization of Stratum Corneum and Transdermal Permeation Pathways, Norris G. Turner and Lourdes B. Nonato X-Ray Analysis of the Stratum Corneum and Its Lipids, Joke A. Bouwstra, Gert S. Gooris, and Stephen H. White Infrared Spectroscopic and Differential Scanning Calorimetric Investigations of the Stratum Corneum Barrier Function, Aarti Naik and Richard H. Guy Investigation of Membrane Structure and Dynamics by Deuterium NMR: Application to the Stratum Corneum, William Abraham, Neil Kitson, Myer Bloom, and Jenifer L. Thewalt Characterization of Stratum Corneum Barrier Properties Using Fluorescence Spectroscopy, Louk A. R. M. Pechtold, William Abraham, and Russell O. Potts Impedance Spectroscopy: Applications to Human Skin, Ronald P. Burnette and John D. DeNuzzio Neutrons, Surfaces, and Skin, Adam C. Watkinson, Jonathan Hadgraft, Paul R. Street, and Randal W. Richards Characterization of the Passive Transdermal Diffusional Route of Polar/Ionic Permeants, Kendall D. Peck and William I. Higuchi Solute Structure as a Determinant of Iontophoretic Transport, Michael Stephen Roberts, Pamela M. Lai, Sheree E. Cross, and Nagahiro H. Yoshida
B Cells of HIV-1–Infected Patients Bind Virions through Cd21–Complement Interactions and Transmit Infectious Virus to Activated T Cells (2000)

The impact of HIV-associated immunopathogenesis on B cells has been largely associated with indirect consequences of viral replication. This study demonstrates that HIV interacts directly with B cells in both lymphoid tissues and peripheral blood. B cells isolated from lymph node and peripheral blood mononuclear cells (PBMCs) of 4 and 23 chronically infected patients, respectively, demonstrated similar capacities to pass virus to activated HIV-negative PBMCs when compared with CD4(+) cells from the same patients. However, in contrast to T cells, virus associated with B cells was surface bound, as shown by its sensitivity to pronase and the staining pattern revealed by in situ amplification of HIV-1 RNA. Cell sorting and ligand displacing approaches established that CD21 was the HIV-binding receptor on B cells, and that this association was mediated through complement-opsonized virus. These B cells were also found to express significantly lower levels of CD21 compared with HIV-negative individuals, suggesting a direct perturbing effect of HIV on B cells. These findings suggest that B cells, although they themselves are not readily infected by HIV, are similar to follicular dendritic cells in their capacity to serve as extracellular reservoirs for HIV-1. Furthermore, B cells possess the added capability of circulating in peripheral blood and migrating through tissues where they can potentially interact with and pass virus to T cells.
Lamins and Lamin-Binding Proteins in Functional Chromatin Organization (1999)

Lamins are the major components of the nuclear lamina, a two-dimensional filamentous network at the periphery of the nucleus in higher eukaryotes, directly underlying the inner nuclear membrane. Several integral proteins of the inner nuclear membrane bind to lamins and may link the nuclear membrane to the core lamina network. The lamins and the lamin-binding proteins lamina-associated polypeptide (LAP)2beta and lamin B receptor (LBR) have been described to bind to DNA or to interact with chromatin via histones, BAF-1, and HP1 chromodomain proteins, respectively, and may provide anchorage sites for chromatin fibers at the nuclear periphery. In addition, lamin A structures on intranuclear filaments, or lamin B in replication foci have been described in the nuclear interior, but their specific roles remain unclear. An isoform of the LAP2 protein family, LAP2alpha, has been found to colocalize with A-type lamins in the nucleoplasm and might be involved in intranuclear structure organization. In the course of cell-cycle-dependent dynamics of the nucleus in higher eukaryotes, lamins as well as lamin-binding proteins seem to possess important functions during various steps of post-mitotic nuclear reassembly, including cross-linking of chromatides, nuclear membrane targeting, nuclear lamina assembly, and the formation of a replication-competent nucleus.
Biologically active Rep proteins of adeno-associated virus type 2 produced as fusion proteins in Escherichia coli (1994)

Four Rep proteins are encoded by the human parvovirus adeno-associated virus type 2 (AAV). The two largest proteins, Rep68 and Rep78, have been shown in vitro to perform several activities related to AAV DNA replication. The Rep78 and Rep68 proteins are likely to be involved in the targeted integration of the AAV DNA into human chromosome 19, and the full characterization of these proteins is important for exploiting this phenomenon for the use of AAV as a vector for gene therapy. To obtain sufficient quantities for facilitating the characterization of the biochemical properties of the Rep proteins, the AAV rep open reading frame was cloned and expressed in Escherichia coli as a fusion protein with maltose-binding protein (MBP). Recombinant MBP-Rep68 and MBP-Rep78 proteins displayed the following activities reported for wild-type Rep proteins when assayed in vitro: (i) binding to the AAV inverted terminal repeat (ITR), (ii) helicase activity, (iii) site-specific (terminal resolution site) endonuclease activity, (iv) binding to a sequence within the integration locus for AAV DNA on human chromosome 19, and (v) stimulation of radiolabeling of DNA containing the AAV ITR in a cell extract. These five activities have been described for wild-type Rep produced from mammalian cell extracts. Furthermore, we recharacterized the sequence requirements for Rep binding to the ITR and found that only the A and A' regions are necessary, not the hairpin form of the ITR.
The generation of intensity-modulated fields for conformal radiotherapy by dynamic collimation (1992)

An algorithm has been developed to calculate the collimator jaw motions required to generate intensity-modulated fields for use in conformal radiotherapy. The dynamic technique allows arbitrary intensity profiles to be generated using a single unidirectional sweep of the collimators. The collimator jaws have independent motion, so that an aperture of variable width is scanned across the field. The algorithm has the form of a constrained optimization problem and jaw motions are optimized such that the treatment time for the field at a given dose rate is minimized. The application and results of this technique are presented, and it is shown that this approach provides an efficient practical implementation of conformal radiotherapy plans based on the use of intensity-modulated fields. The technique can be extended to 3D treatment plans and fields through the use of computer-controlled multileaf collimators.
Pathogenic YeastsCryptococcus neoformansandCandida albicansProduce Immunomodulatory Prostaglandins (2001)

Enhanced prostaglandin production during fungal infection could be an important factor in promoting fungal colonization and chronic infection. Host cells are one source of prostaglandins; however, another potential source of prostaglandins is the fungal pathogen itself. Our objective was to determine if the pathogenic yeasts Cryptococcus neoformans and Candida albicans produce prostaglandins and, if so, to begin to define the role of these bioactive lipids in yeast biology and disease pathogenesis. C. neoformans and C. albicans both secreted prostaglandins de novo or via conversion of exogenous arachidonic acid. Treatment with cyclooxygenase inhibitors dramatically reduced the viability of the yeast and the production of prostaglandins, suggesting that an essential cyclooxygenase like enzyme may be responsible for fungal prostaglandin production. A PGE series lipid was purified from both C. albicans and C. neoformans and was biologically active on both fungal and mammalian cells. Fungal PGE(x) and synthetic PGE(2) enhanced the yeast-to-hypha transition in C. albicans. Furthermore, in mammalian cells, fungal PGE(x) down-modulated chemokine production, tumor necrosis factor alpha production, and splenocyte proliferation while up-regulating interleukin 10 production. These are all activities previously documented for mammalian PGE(2). Thus, eicosanoids are produced by pathogenic fungi, are critical for growth of the fungi, and can modulate host immune functions. The discovery that pathogenic fungi produce and respond to immunomodulatory eicosanoids reveals a virulence mechanism that has potentially great implications for understanding the mechanisms of chronic fungal infection, immune deviation, and fungi as disease cofactors.
Herbal Medicines and Perioperative Care (2001)

During the preoperative evaluation, physicians should explicitly elicit and document a history of herbal medication use. Physicians should be familiar with the potential perioperative effects of the commonly used herbal medications to prevent, recognize, and treat potentially serious problems associated with their use and discontinuation.