gebhart/patrepeval huggingface dataset:
High oxygen-reduction activity and durability of nitrogen-doped graphene (2011)

Nitrogen-doped graphene as a metal-free catalyst for oxygen reduction was synthesized by heat-treatment of graphene using ammonia. It was found that the optimum temperature was 900 °C. The resulting catalyst had a very high oxygen reduction reaction (ORR) activity through a four-electron transfer process in oxygen-saturated 0.1 M KOH. Most importantly, the electrocatalytic activity and durability of this material are comparable or better than the commercial Pt/C (loading: 4.85 µgPt cm−2). XPS characterization of these catalysts was tested to identify the active N species for ORR.
Phage display and bacterial expression of a recombinant Fab specific for Pseudomonas aeruginosa serotype O6 lipopolysaccharide (1997)

Immunotherapy with antibodies (Abs) against the lipopolysaccharide (LPS) of Pseudomonas aeruginosa remains an alternative to serotype-specific LPS-based vaccines due to their limited use and to antibiotics due to the intrinsic resistance to antimicrobials observed in P. aeruginosa. We have chosen a monoclonal Ab (MAb), MF23-1, that binds to the O antigen of the most clinically relevant serotype, IATS O6, for producing a recombinant antibody. Heavy (H) and light (L) chain genes were isolated from MF23-1 to form a functional Fab molecule in the periplasm of Escherichia coli and on the surface of phage by using phagemid vector pComb3. The entire kappa L chain gene was used, but the H chain gene was amplified to 2 amino acids past cysteine 128 which is involved in interchain disulfide bond formation with the L chain. The truncated H chain associated with the L chain in the periplasm of E. coli to form a functional Fab molecule that bound in both enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay to O6 LPS. Therefore, the remainder of the CH1 past cysteine 128 is not essential for stable formation of the Fab portion of MF23-1. This recombinant Fab (r-Fab) was shown to be specific for the LPS of the most predominant clinical isolate, serotype O6, while no cross-reactivity was detected to the LPS of the other 19 remaining serotypes. This r-Fab was also expressed on the surface of filamentous phage upon addition of helper phage to recombinant E. coli containing phagemid. Recombinant phage from clones MT13 and MT24 bound specifically to O6 LPS in ELISA. These results represent an important step toward the design of therapeutic Abs to be used against P. aeruginosa infections.
Prediction of Offshore Viscous Oil Field Performance (1999)

Development plans for UKCS viscous oil reservoirs use production profiles predicted by fullfield simulation models. The use of horizontal wells, possibly combined with other IOR techniques, and the unusually high vertical permeability of many of the fields, lead to a range of issues that need to be carefully considered when building simulation models and integrating laboratory data. This paper gives an overview of experience in modelling UKCS viscous oil fields, highlighting key issues that need to be addressed.
<title>Laser removal of surface and embedded contaminations on/in building structures</title> (1994)

This paper demonstrates the technical feasibility and basic phenomena of using laser techniques for the non-contact removal of embedded contamination down to depths of 0.1 - 4 mm thick in construction materials such a concrete, brick, plaster/mortar, stones and stainless/mild steels. In this study a high power CO<SUB>2</SUB> laser and a YAG laser were used. The techniques investigated include laser vaporization removal, laser combustion/decomposition removal, laser melt ejection removal, laser thermal fracture removal and laser HAZ delamination removal. The work showed that melt ejection removal can be applied to metal objects with removal depth up to 1.5 mm/pulse while the other four methods are more effective for nonmetallic materials with removal depth up to 3 mm for each pass. Particularly when hydraulic bond materials such as concrete, cement, mortar/plaster, rendering and stones are involved the thermal fracture and HAZ delamination methods were found very effective. Paint, epoxy, and plants such as moss and lichen on the construction bodies can be removed effectively by laser generated combustion/combustion. One of the advantages of laser contamination removal is the energy controllability, remote operation capability (convenient for nuclear decontamination), low waste and high efficiency. In addition amorphous glazing can be generated on the surfaces of construction materials such as bricks and concrete during vaporization removal providing a means of sealing the remaining surface. Optical microscopy, SEM, EDAX, and x-ray diffraction were used to study the affects of laser treatment under various conditions. Mathematical representation of the processes were discussed. Comparison was made between the methods and optimum operating condition provided.
Elimination of p19ARF-expressing cells enhances pulmonary function in mice (2016)

Senescent cells accumulate in many tissues as animals age and are considered to underlie several aging-associated pathologies. The tumor suppressors p19<sup>ARF</sup> and p16<sup>INK4a</sup>, both of which are encoded in the <i>CDKN2A</i> locus, play critical roles in inducing and maintaining permanent cell cycle arrest during cellular senescence. Although the elimination of p16<sup>INK4a</sup>-expressing cells extends the life span of the mouse, it is unclear whether tissue function is restored by the elimination of senescent cells in aged animals and whether and how p19<sup>ARF</sup> contributes to tissue aging. The aging-associated decline in lung function is characterized by an increase in compliance as well as pathogenic susceptibility to pulmonary diseases. We herein demonstrated that pulmonary function in 12-month-old mice was reversibly restored by the elimination of p19<sup>ARF</sup>-expressing cells. The ablation of p19<sup>ARF</sup>-expressing cells using a toxin receptor-mediated cell knockout system ameliorated aging-associated lung hypofunction. Furthermore, the aging-associated gene expression profile was reversed after the elimination of p19<sup>ARF</sup>. Our results indicate that the aging-associated decline in lung function was, at least partly, attributed to p19<sup>ARF</sup> and was recovered by eliminating p19<sup>ARF</sup>-expressing cells.
Formation and Elution of Toxic Compounds from Sterilized Medical Products: Methylenedianiline Formation in Polyurethane (1995)

The formation of a toxic and carcinogenic compound, methylenedianiline (MDA), in sterilized medical use polyurethane (PU) is discussed. Due to good biocompatibility and biostability, PU is widely used for blood-containing devices. There are two types of PU currently available for medical use. One is chain-extended thermoplastic PU, the other is thermosetting PU used for potting material connecting fibers and modules in artificial dialyzers and plasma separators. Both gamma-ray irradiation and autoclave sterilization are predominantly used for the sterilization of these devices. MDA formation in sterilized PUs by gamma-ray irradiation and by autoclave treatment is compared. The Delany clause in the USA prohibits the manufacture of medical devices producing any toxic compound during fabrication and sterilization, therefore, the formation and elution of MDA and other toxic compounds should be seriously considered. Although MDA formation at a concentration of a few to a few hundred ppb in autoclaved chain-extended thermoplastic PU has been reported, there have been no papers describing MDA formation in autoclaved thermosetting PU potting material, or describing MDA formation in gamma-ray irradiated chain-extended thermoplastic PU and thermosetting PU. We elected to determine whether MDA was in fact produced in Pus sterilized by gamma-ray irradiation or by autoclave sterilization. Our objective was to estimate the risk factor to the human patients or recipient. Our conclusion is to confirm which sterilization of gamma-ray or autoclave is more appropriate. No formation of MDA was observed in autoclaved thermosetting PU potting material at 121 degrees C for 60 min. A few ppm of MDA was formed in irradiated potting material. MDA formation increased with increasing irradiation doses. MDA formed in irradiated potting material at 2.5 Mrad (less than one ppm) is not a significant risk to the recipient. The estimated cancer causing risk factor when absorbing one mg MDA/kg body weight/day is 0.29. Twenty-nine persons per 100 persons exhibit a cancer potential. More importantly, our experimental evidence indicated that serum extracts from irradiated potting material contained unknown compounds other than MDA. The presence of these compounds is considerably more significant and toxic. When tested, these compounds proved to be mutagenic in the absence of metabolic activity, thus promoting a need to identify and study further. Compounds indicating mutagenicity increased with increasing irradiation. Small amounts of mutagenic compounds were determined in extract from autoclaved potting material, smaller than that by gamma-ray irradiation. Therefore, autoclave sterilization is more appropriate, providing materials can withstand the process.(ABSTRACT TRUNCATED AT 400 WORDS)
Synthesis and sorting of proteoglycans (2000)

Proteoglycans are widely expressed in animal cells. Interactions between negatively charged glycosaminoglycan chains and molecules such as growth factors are essential for differentiation of cells during development and maintenance of tissue organisation. We propose that glycosaminoglycan chains play a role in targeting of proteoglycans to their proper cellular or extracellular location. The variability seen in glycosaminoglycan chain structure from cell type to cell type, which is acquired by use of particular Ser-Gly sites in the protein core, might therefore be important for post-synthesis sorting. This links regulation of glycosaminoglycan synthesis to the post-Golgi fate of proteoglycans.
Pathogenesis of cerebral microbleeds: In vivo imaging of amyloid and subcortical ischemic small vessel disease in 226 individuals with cognitive impairment (2013)

Our findings suggest that although deep CMBs are mainly linked to subcortical SVD, both subcortical SVD and amyloid-related pathologies (eg, CAA) contribute to the pathogenesis of lobar CMBs, at least in subjects with mixed lobar and deep CMBs. Furthermore, subcortical SVD and amyloid-related pathologies interact to increase the risk of lobar CMBs.
Substrate specificity of the isoprenylated protein endoprotease (1992)

Proteins containing a CAAX motif at their carboxyl termini are subject to isoprenylation at the cysteine residue. Proteolytic trimming of isoprenylated proteins is essential in the activation of these proteins. A microsomal endopeptidase activity has been identified which cleaves all-trans farnesylated cysteine containing tetrapeptides between the modified residue and the adjacent amino acid to liberate the modified cysteine residue and an intact tripeptide. Structure/activity studies are reported here on this endopeptidase activity which are consistent with the premise that this protease is identical to the one normally involved in the cellular isoprenylation pathway. The protease only processes peptides which possess an isoprenyl moiety. Within the isoprenyl series, the enzyme hydrolyzes all-trans-farnesyl-, all-trans-geranylgeranyl-, and geranyl-containing peptides. The protease also recognizes the AAX sequence, because the protease behaves either stereospecifically or stereoselectively with respect to the individual amino acids of the tripeptide. The enzyme only measurably hydrolyzes isoprenylated peptides possessing L-amino acids at C and A. On the other hand, there is a small but measurable hydrolysis of isoprenylated peptides containing a D-amino acid at X.
Synthesis and biological evaluation of conformationally restricted 2-(1-pyrrolidinyl)-N-[2-(3,4-dichlorophenyl)ethyl]-N-methylethylenediamines as .sigma. receptor ligands. 1. Pyrrolidine, piperidine, homopiperidine, and tetrahydroisoquinoline classes (1992)

The synthesis and sigma receptor affinity of a series of conformationally restricted derivatives of 2-(1-pyrrolidinyl)-N-[2-(3,4-dichlorophenyl)ethyl]-N-methylethylenedi amine (1) is described. The pyrrolidinyl (or N,N-dialkyl),ethylenediamine,N-alkyl, and phenylethyl portions of this sigma receptor pharmacophore were restricted by its incorporation into 1,2-cyclohexanediamine-, pyrrolidine-, piperidine-, homopiperidine-, and tetrahydroisoquinoline-containing ligands. The sigma receptor binding affinities of these compounds were determined using [3H](+)-pentazocine in guinea pig brain homogenates. The synthesis of all but one class was achieved by acylation and alane reduction of the appropriate diamine precursors whose synthesis is also reported. sigma receptor affinities ranged from 1.34 nM for 6,7-dichloro-2-[2-(1-pyrrolidinyl)ethyl]tetrahydroisoquinoline (12) to 455 nM for (1R,2R)-trans-N-[2-(3,4-dichlorophenyl)ethyl]-N-methyl-2- (1-pyrrolidinyl)cyclohexylamine [(-)-4]. In this displacement assay, (+)-pentazocine exhibited a Ki of 3.1 nM while DTG and haloperidol showed Ki values of 27.7 and 3.7 nM, respectively. The conformationally free parent compound 1 exhibited a Ki value of 2.1 nM. Comparison of both the sigma receptor affinities and nitrogen atom geometry of the compounds revealed that a gauche relation of the nitrogen atoms of cis-1,2-cyclohexanediamines is not imperative for high affinity as we had previously thought. It is highly likely that nitrogen lone pair orientations and steric factors on the aliphatic portions of these ligands play a major role in the sigma receptor binding of this pharmacophore.
A Unique Matched Quadruplet of Terbium Radioisotopes for PET and SPECT and for α- and β−-Radionuclide Therapy: An In Vivo Proof-of-Concept Study with a New Receptor-Targeted Folate Derivative (2012)

For the first time, to our knowledge, 4 terbium radionuclides have been tested in parallel with tumor-bearing mice using an FR targeting agent. Along with excellent tumor visualization enabled by (152)Tb PET and (155)Tb SPECT, we demonstrated the therapeutic efficacy of the α-emitter (149)Tb and β(-)-emitter (161)Tb.
A Survey of Open-World Person Re-Identification (2019)

Person re-identification (re-ID) has been a popular topic in computer vision and pattern recognition communities for a decade. Several important milestones such as metric-based and deeply-learned re-ID in recent years have promoted this topic. However, most existing re-ID works are designed for closed-world scenarios rather than realistic open-world settings, which limits the practical application of the re-ID technique. On one hand, the performance of the latest re-ID methods has surpassed the human-level performance on several commonly used benchmarks (e.g., Market1501 and CUHK03), which are collected from closed-world scenarios. On the other hand, open-world tasks that are less developed and more challenging have received increasing attention in the re-ID community. Therefore, this paper starts the first attempt to analyze the trends of open-world re-ID and summarizes them from both narrow and generalized perspectives. In the narrow perspective, open-world re-ID is regarded as person verification (i.e., open-set re-ID) instead of person identification, that is, the query person may not occur in the gallery set. In the generalized perspective, application-driven methods that are designed for specific applications are defined as generalized open-world re-ID. Their settings are usually close to realistic application requirements. Specifically, this survey mainly includes the following four points for open-world re-ID: 1) analyzing the discrepancies between closed- and open-world scenarios; 2) describing the developments of existing open-set re-ID works and their limitations; 3) introducing specific application-driven works from three aspects, namely, raw data, practical procedure, and efficiency; and 4) summarizing the state-of-the-art methods and future directions for open-world re-ID. This survey on open-world re-ID provides a guidance for improving the usability of re-ID technique in practical applications.
Thin-film quasi-SOI power MOSFET fabricated by reversed silicon wafer direct bonding (1998)

A quasi-SOI power MOSFET has been fabricated by reversed silicon wafer direct bonding. In this power MOSFET, the buried oxide under the channel and source regions is removed and the channel region is directly connected to the source body contact electrode to reduce the base resistance of the parasitic npn bipolar transistor. The quasi-SOI power MOSFET can suppress the parasitic bipolar action and shows lower specific on-resistance than that of the conventional SOI power MOSFET. The fabricated chip level quasi-SOI power MOSFET shows the specific on-resistance of 86 m/spl Omega//spl middot/mm/sup 2/ and on-state breakdown voltage of 30 V.
Cell to Cell Signalling via Exosomes Through esRNA (2007)

Exosomes are small vesicles of endosomal origin that can be released by many different cells to the microenvironment. Exosomes have been shown to participate in the immune system, by mediating antigen presentation. We have recently shown the presence of both mRNA and microRNA in exosomes, specifically in exosomes derived from mast cells. This RNA can be transferred between one mast cell to another, most likely through fusion of the exosome to the recipient cell membrane. The delivered RNA is functional, as the mRNA can lead to translation of new proteins in a recipient cell. The RNA shuttled between cells via exosomes is called esRNA. We propose that several types of exosomes may exist, and that an additional function of exosomes is to communicate to neighbouring cells through delivery of RNA-signals.
An overview of algae bioethanol production (2014)

Because of rapid growth in population and industrialization, worldwide ethanol demand is increasing continuously. The first-generation and second-generation biofuels are unable to meet the global demand of bioethanol production because of their primary value of food and feed. Therefore, algae are among the most potentially significant sources of sustainable biofuels in the future of renewable energy because of the accumulating high starch/cellulose and because they are widely distributed in nature. The focus of this paper is to review the production and recent advances in research and development in the algae bioethanol, including pretreatment, hydrolysis, and fermentation of algae biomass. Despite the many developments made in the recent years, commercialization of algal bioethanol remains challenging chiefly because of the techno-economic constraints. Technological breakthroughs in all major aspects must be overcome before it can be a successfully large-scale and commercialized product. Copyright © 2014 John Wiley & Sons, Ltd.
Cloning of ligand-specific cell lines via gene transfer: identification of a D2 dopamine receptor subtype. (1989)

Using rat genomic DNA, we have established a transfected mouse fibroblast cell line that expresses a spiperone binding site with the pharmacological characteristics of a D2 dopamine receptor. The expressed D2 receptors are the product of a gene that is distinct from that reported by Bunzow et al. [Bunzow, J. R., Van Tol, H. H. M., Granoly, D. K., Albert, P., Salon, J., Christie, M., Machida, C. A., Neve, K. A. & Civelli, O. (1988) Nature (London) 336, 783-787]. Flow cytometry with the Ca2+-sensitive dye indo-1 demonstrated that activation of the expressed D2 sites resulted in increases in intracellular calcium that were dependent on the influx of external Ca2+. These general cloning procedures should be applicable to the production of cell lines expressing a variety of genes for which only functional assays are available.
Immunogenicity Testing by Electrochemiluminescent Detection for Antibodies Directed against Therapeutic Human Monoclonal Antibodies (2005)

Monitoring the immune response against human therapeutic monoclonal antibodies is an important component of preclinical and clinical trials to assess drug exposure, efficacy, and safety (1)(2). Detection of antibodies (analyte) directed against therapeutic antibodies (drug) is difficult because of the similarity of structure between drug and analyte and the high concentrations of drug present in serum, particularly during preclinical toxicology studies (3)(4). The purpose of this study was to develop a robust assay format applicable across species and drug compounds for rapid method development and validation.

We developed 5 assays to detect antibodies against 5 distinct monoclonal antibody therapeutic drug products. Detection was dependent on bivalent antibodies binding both biotin- and ruthenium-conjugated drug compounds (for a schematic of the assay, see Fig. 1 of the Data Supplement that accompanies the online version of this abstract at http://www.clinchem.org/content/vol51/issue10/). This “bridging” assay format eliminated the need for species–specific secondary antibodies and provided assay consistency in different serum matrices as a drug product moves from preclinical to clinical development. …
Cloning and expression of foreign genes in vaccinia virus, using a host range selection system (1989)

A simple selection system has been developed for the cloning and expression of open reading frames in vaccinia virus. The selection system is based on a conditional lethal (host range) mutant of vaccinia virus. A deletion mutant of the vaccinia virus WR strain was generated by insertion of the neomycin resistance gene from transposon Tn5 and selection with the antibiotic G418. This deletion recombinant, vP293, lacked approximately 21.7 kilobases of DNA beginning 3.8 kilobases from the left end of the genome, vP293, was capable of plaquing on primary chicken embryo fibroblasts and two monkey cell lines (BSC-40 and Vero) but was defective in replication in the human cell line MRC-5. Insertion of the host range gene K1L into vP293 restored the ability to grow on MRC-5 cells. A series of plasmids were constructed which in addition to the K1L gene contained a vaccinia virus early-late promoter, H6, followed by a unique polylinker sequence, translational initiation and termination signals, and an early transcription termination signal. These plasmids, pHES1 through 4, allowed for rapid single-step cloning and expression of any open reading frame when recombined in vivo with vP293 and scored for growth on MRC-5 cells.
Cloning of an aroF allele encoding a tyrosine-insensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (1990)

In Escherichia coli, genes aroF+, aroG+, and aroH+ encode isoenzymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases that are feedback inhibited by tyrosine, phenylalanine, and tryptophan, respectively. A single base pair change in aroF causes a Pro-148-to-Leu-148 substitution and results in a tyrosine-insensitive enzyme.