gebhart/patrepeval huggingface dataset:
Oestrogens and the Structural and Functional Plasticity of Neurons: Implications for Memory, Ageing and Neurodegenerative Processes (2007)

Oestrogens have numerous effects on the brain, beginning during gestation and continuing on into adulthood. Many of these actions involve areas of the brain that are not primarily involved in reproduction, such as the basal forebrain, hippocampus, caudate putamen, midbrain raphe and brainstem locus coeruleus. This paper describes three actions of oestrogens that are especially relevant to brain mechanisms involved in memory processes and their alterations during ageing and neurodegenerative diseases: (1) the regulation of cholinergic neurons by oestradiol in the rat basal forebrain, involving induction of choline acetyltransferase and acetylcholinesterase according to a sexually dimorphic pattern; (2) the regulation of synaptogenesis in the CA1 region of the hippocampus by oestrogens and progestins during the four- to five-day oestrus cycle of the female rat. Formation of new excitatory synapses is induced by oestradiol and involves N-methyl-D-aspartate receptors; removal of these synapses involves intracellular progestin receptors; (3) sex differences in hippocampal structure, which may help to explain differences in the strategies that male and female rats use to solve spatial navigation problems. During the period of development when testosterone is elevated in the male, aromatase and oestrogen receptors are also elevated, making it likely that this pathway is involved in the masculinization of hippocampal structure.
CONTROLLED TRIAL OF FRUSEMIDE AS AN ANTIEPILEPTIC DRUG IN FOCAL EPILEPSY (1976)

The antiepileptic activity of oral frusemide (120 mg daily) was compared with that of an identical placebo in a double-blind crossover trial in fourteen patients with severe focal epilepsy who were receiving long-term therapy with established antiepileptic drugs. A statistically significant reduction in the frequency of focal fits was seen with the active drug. Marked drowsiness occurred in three patients during frusemide therapy, causing their withdrawal from the trial. A slight, but significant, rise in serum phenobarbitone concentrations was observed during frusemide therapy, but no change was seen in serum primidone or phenytoin concentrations. Frusemide significantly lowered plasma sodium and potassium concentrations, and increased plasma bicarbonate.
Pooled Platelet Concentrates Prepared by the Platelet-Rich-Plasma Method and Filtered with Three Different Filters and Stored for 8 Days (1995)

Pooled platelet concentrates (PC) prepared by the platelet-rich plasma (PRP)method were filtered with three different filters and stored for 8 days at room temperature. The effect of filtration on leukocyte contamination, platelet concentration,and the in vitro function, morphology, metabolism and activation of platelets were studied. Eight pools of 20 PRP-PC were used, each pool was split into 4 equal volumes; 3 were filtered over a PL50HF, a PL-10A and a Bio PIO filter,the 4 served as a control. After filtration, leukocyte counts exceeded 3x10^5 in none of the pooled PC. Platelet loss induced by filtration was about 17%. During storage, no differences in pH, PC02, and lactate and glucose concentration were found between the filtered and the unfiltered units, nor were any differences observed between filtered and unfiltered pooled PC in aggregation upon stimulation with collagen and/or ADP, adhesion capacity to collagen in flowing blood,nucleotide content of the platelets and nucleobase concentration in the plasma,expression of activation-dependent antigens, or platelet morphology as observed by light microscopy and by the swirling effect. Selective removal of β-thromboglobulin(22%) by the PL50HF filter was observed. Pooled PC prepared by the PRP-method can be filtered and stored for 8 days without detrimental effect on platelet function, metabolism or activation.
Capabilities of low power excimer lasers in micromachining (2002)

Small excimer lasers in combination with high-resolution imaging and precision motion control systems are capable of direct-write shaping of many polymers, ceramics, glasses, and metal films. Microfabrication workstations of this type are often characterized by size, cost, and facilities requirements compatible with the resources of most laboratories and speciality shops involved in development or small-batch manufacturing of microstructures. Use of deep-up laser wavelengths allows application of ablation processes to a broad range of materials, and the maskless, direct-write fabrication method is well-suited to rapid set-up and change-over. In addition, the direct-write scheme can be employed to fabricate truly three dimensional microstructures with continuous variation of surface height and good surface finish.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
PTEN/MMAC1 mutations in endometrial cancers. (1997)

Endometrial carcinomas represent the most common gynecological cancer in the United States, yet the molecular genetic events that underlie the development of these tumors remain obscure. Chromosome 10 is implicated in the pathogenesis of endometrial carcinoma based on loss of heterozygosity (LOH), comparative genomic hybridization, and cytogenetics. Recently, a potential tumor suppressor gene, PTEN/MMAC1, with homology to dual-specificity phosphatases and to the cytoskeletal proteins tensin and auxillin was identified on chromosome 10. This gene is mutated in several types of advanced tumors that display frequent LOH on chromosome 10, most notably glioblastomas. Additionally, germ-line mutations of PTEN/MMAC1 are responsible for several familial neoplastic disorders, including Cowden disease and Bannayan-Zonana syndrome. Because this locus is included in the region of LOH in many endometrial carcinomas, we examined 70 endometrial carcinomas for alterations in PTEN/MMAC1. Somatic mutations were detected in 24 cases (34%) including 21 cases that resulted in premature truncation of the protein, 2 tumors with missense alterations in the conserved phosphatase domain, and 1 tumor with a large insertion. These data indicate that PTEN/MMAC1 is more commonly mutated than any other known gene in endometrial cancers.
Backbone Amide Linker (BAL) Strategy forNα-9-Fluorenylmethoxycarbonyl (Fmoc) Solid-Phase Synthesis of Unprotected Peptidep-Nitroanilides and Thioesters1 (1999)

A novel and general backbone amide linker (BAL) strategy has been devised for preparation of C-terminal modified peptides containing hindered, unreactive, and/or sensitive moieties, in concert with N(alpha)()-9-fluorenylmethoxycarbonyl (Fmoc) solid-phase synthesis protocols. This strategy comprises (i) start of peptide synthesis by anchoring the penultimate residue, with its carboxyl group orthogonally protected, through the backbone nitrogen, (ii) continuation with standard protocols for peptide chain elongation in the C --> N direction, (iii) selective orthogonal removal of the carboxyl protecting group, (iv) solid-phase activation of the pendant carboxyl and coupling with the desired C-terminal residue, and (v) final cleavage/deprotection to release the free peptide product into solution. To illustrate this approach, several model peptide p-nitroanilides and thioesters have been prepared in excellent yields and purities, with minimal racemization. Such compounds are very difficult to prepare by standard Fmoc chemistry, including the BAL strategy as originally envisaged.
A New Mouse Liver-specific Gene, Encoding a Protein Homologous to Human Antimicrobial Peptide Hepcidin, Is Overexpressed during Iron Overload (2001)

Considering that the development of hepatic lesions related to iron overload diseases might be a result of abnormally expressed hepatic genes, we searched for new genes up-regulated under the condition of iron excess. By suppressive subtractive hybridization performed between livers from carbonyl iron-overloaded and control mice, we isolated a 225-base pair cDNA. By Northern blot analysis, the corresponding mRNA was confirmed to be overexpressed in livers of experimentally (carbonyl iron and iron-dextran-treated mice) and spontaneously (beta(2)-microglobulin knockout mice) iron-overloaded mice. In addition, beta(2)-microglobulin knockout mice fed with a low iron content diet exhibited a decrease of hepatic mRNA expression. The murine full-length cDNA was isolated and was found to encode an 83-amino acid protein presenting a strong homology in its C-terminal region to the human antimicrobial peptide hepcidin. In addition, we cloned the corresponding rat and human orthologue cDNAs. Both mouse and human genes named HEPC are constituted of 3 exons and 2 introns and are located on chromosome 7 and 19, respectively, in close proximity to USF2 gene. In mouse and human, HEPC mRNA was predominantly expressed in the liver. During both in vivo and in vitro studies, HEPC mRNA expression was enhanced in mouse hepatocytes under the effect of lipopolysaccharide. Finally, to analyze the intracellular localization of the predicted protein, we used the green fluorescent protein chimera expression vectors. The murine green fluorescent protein-prohepcidin protein was exclusively localized in the nucleus. When the putative nuclear localization signal was deleted, the resulting protein was addressed to the cytoplasm. Taken together, our data strongly suggest that the product of the new liver-specific gene HEPC might play a specific role during iron overload and exhibit additional functions distinct from its antimicrobial activity.
Error control and concealment for video communication: a review (1998)

The problem of error control and concealment in video communication is becoming increasingly important because of the growing interest in video delivery over unreliable channels such as wireless networks and the Internet. This paper reviews the techniques that have been developed for error control and concealment. These techniques are described in three categories according to the roles that the encoder and decoder play in the underlying approaches. Forward error concealment includes methods that add redundancy at the source end to enhance error resilience of the coded bit streams. Error concealment by postprocessing refers to operations at the decoder to recover the damaged areas based on characteristics of image and video signals. Last, interactive error concealment covers techniques that are dependent on a dialogue between the source and destination. Both current research activities and practice in international standards are covered.
Purification and characterization of class II histocompatibility antigens from a homozygous human B cell line. (1987)

Human class II histocompatibility antigens were purified from the Epstein-Barr virus-transformed human B lymphoblastoid cell line LG-2 by immunoaffinity chromatography. This is the first time all three subsets have been prepared as nonradioactive materials on a milligram scale. The yields of DR, DQ, and DP from 10 g of cells were approximately 12, 2, and 0.2 mg, respectively. Cross-contamination of the subsets was found to be less than 2% when assayed by measuring the binding of antigen-specific monoclonal antibodies to antigen immobilized on fixed erythrocytes. The three purified subsets were extensively characterized. They contained no detectable invariant chain. The three proteins were distinguished by their migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing. The denatured antigens were susceptible to partial removal of carbohydrate by endoglycosidase H and apparently complete removal of carbohydrate by endoglycosidase F. The isolated, denatured chains differed in their affinities for radiolabeled lectins, suggesting differences in carbohydrate structures. A water-soluble form of each antigen was prepared by a controlled papain digestion of the native antigen. Both native and denatured antigens were analyzed for their reactivities with a panel of class II antigen-specific monoclonal antibodies, allowing a precise definition of the specificities of the antibodies.
A Micro Aerosol Sensor for the Measurement of Airborne Ultrafine Particles (2016)

Particle number concentration and particle size are the two key parameters used to characterize exposure to airborne nanoparticles or ultrafine particles that have attracted the most attention. This paper proposes a simple micro aerosol sensor for detecting the number concentration and particle size of ultrafine particles with diameters from 50 to 253 nm based on electrical diffusion charging. The sensor is composed of a micro channel and a couple of planar electrodes printed on two circuit boards assembled in parallel, which thus integrate charging, precipitating and measurement elements into one chip, the overall size of which is 98 × 38 × 25 mm³. The experiment results demonstrate that the sensor is useful for measuring monodisperse aerosol particles with number concentrations from 300 to 2.5 × 10⁴ /cm³ and particle sizes from 50 to 253 nm. The aerosol sensor has a simple structure and small size, which is favorable for use in handheld devices.
Design and construction of a light-delivery system for photodynamic therapy (1999)

We have developed a device to divide the output from a dye laser into as many as eight beams of equal power with negligible total power loss. In this system, 630-nm s-plane polarized laser light was split by a series of highly polarization-sensitive plate beamsplitters. Each of the beams was coupled to a 200, 400, or 600 microm diameter optical fiber. Brewster-window-type attenuators allowed the power of each beam to be individually set. It was possible to reconfigure the device to produce four, two, or one output(s). We discuss the design requirements of the beamsplitter device and describe its construction from mostly commercially available components. An apparatus for positioning and stabilizing each optical fiber relative to the skin surface of a patient is also described. The illumination from the fiberoptic supported by such an apparatus strikes a defined surface area and is independent of patient movement. Both the beamsplitter device and the optical fiber positioner are used routinely in photodynamic therapy (PDT) of malignant tumors in the clinic and in the laboratory.
Slow-Release 5-Aminosalicylic Acid Therapy in Children with Small Intestinal Crohnʼs Disease (1993)

Pharmacologic agents effective in the treatment of Crohn's disease confined to the small intestine are limited. The therapeutic efficacy of oral mesalazine in small bowel inflammation, although theoretically promising, remains unproven. In an open-labeled initial trial, timed-release 5-aminosalicylic acid (5-ASA), administered at a daily dosage of 30.6 +/- 9.0 mg/kg (mean +/- SEM) to children with active Crohn's disease involving the small intestine, was associated with improvement on the Harvey index in six of 12 patients treated for 8.1 +/- 3.9 weeks. In a subsequent prospective, double-blind study 14 children, ages 9.3 to 16.1 years, with active Crohn's disease limited radiologically in the small intestine were randomized to receive either timed-release 5-ASA [50 mg/kg/day (maximum 3 g/day)] or placebo for 8 weeks. Following a 4-week washout period, patients crossed over to receive the other study drug for a further 8 weeks. Six children completed the entire 20-week trial. The van Hees index improved among patients receiving 5-ASA for 8 weeks (delta = -18 +/- 6.4) but deteriorated among patients given placebo (delta = +14 +/- 4.1) (p < 0.05). Mean Crohn's Disease Activity Index (CDAI) decreased marginally after 8 weeks of 5-ASA treatment (delta = -48 +/- 38.2) but not with placebo (delta = -3.0 +/- 7.9) (p = 0.31). Of the eight noncompleters, more patients dropped out of the study because of lack of therapeutic response to placebo (n = 5) than to 5-ASA (n = 2).(ABSTRACT TRUNCATED AT 250 WORDS)
Activation of the γ-Tubulin Complex by the Mto1/2 Complex (2014)

The multisubunit γ-tubulin complex (γ-TuC) is critical for microtubule nucleation in eukaryotic cells, but it remains unclear how the γ-TuC becomes active specifically at microtubule-organizing centers (MTOCs) and not more broadly throughout the cytoplasm. In the fission yeast Schizosaccharomyces pombe, the proteins Mto1 and Mto2 form the Mto1/2 complex, which interacts with the γ-TuC and recruits it to several different types of cytoplasmic MTOC sites. Here, we show that the Mto1/2 complex activates γ-TuC-dependent microtubule nucleation independently of localizing the γ-TuC. This was achieved through the construction of a "minimal" version of Mto1/2, Mto1/2[bonsai], that does not localize to any MTOC sites. By direct imaging of individual Mto1/2[bonsai] complexes nucleating single microtubules in vivo, we further determine the number and stoichiometry of Mto1, Mto2, and γ-TuC subunits Alp4 (GCP2) and Alp6 (GCP3) within active nucleation complexes. These results are consistent with active nucleation complexes containing ∼13 copies each of Mto1 and Mto2 per active complex and likely equimolar amounts of γ-tubulin. Additional experiments suggest that Mto1/2 multimers act to multimerize the fission yeast γ-tubulin small complex and that multimerization of Mto2 in particular may underlie assembly of active microtubule nucleation complexes.
Linked: The New Science of Networks (2003)

The first book to explore the hot science of networks and their impact on nature, business, medicine, and everyday life. }In the 1980's, James Gleick's Chaos introduced the world to complexity. Now, Albert-Lszl Barabsi's Linked reveals the next major scientific leap: the study of networks. We've long suspected that we live in a small world, where everything is connected to everything else. Indeed, networks are pervasive--from the human brain to the Internet to the economy to our group of friends. These linkages, it turns out, aren't random. All networks, to the great surprise of scientists, have an underlying order and follow simple laws. Understanding the structure and behavior of these networks will help us do some amazing things, from designing the optimal organization of a firm to stopping a disease outbreak before it spreads catastrophically.In Linked, Barabsi, a physicist whose work has revolutionized the study of networks, traces the development of this rapidly unfolding science and introduces us to the scientists carrying out this pioneering work. These new cartographers are mapping networks in a wide range of scientific disciplines, proving that social networks, corporations, and cells are more similar than they are different, and providing important insights into the interconnected world around us. This knowledge, says Barabsi, can shed light on the robustness of the Internet, the spread of fads and viruses, even the future of democracy. Engaging and authoritative, Linked provides an exciting preview of the next century in science, guaranteed to be transformed by these amazing discoveries.From Linked:This book has a simple message: think networks. It is about how networks emerge, what they look like, and how they evolve. It aims to develop a web-based view of nature, society, and technology, providing a unified framework to better understand issues ranging from the vulnerability of the Internet to the spread of diseases. Networks are present everywhere. All we need is an eye for them...We will see the challenges doctors face when they attempt to cure a disease by focusing on a single molecule or gene, disregarding the complex interconnected nature of the living matter. We will see that hackers are not alone in attacking networks: we all play Goliath, firing shots at a fragile ecological network that, without further support, could soon replicate our worst nightmares by turning us into an isolated group of species...Linked is meant to be an eye-opening trip that challenges you to walk across disciplines by stepping out of the box of reductionism. It is an invitation to explore link by link the next scientific revolution: the science of networks. }
Preparation of composite fluoropolymers with enhanced dewetting using fluorinated silsesquioxanes as drop-in modifiers (2010)

Composite fluoropolymers were prepared by solvent blending fluorinated POSS (F-POSS) with perfluorocyclobutyl (PFCB) aryl ether polymers. The semifluorinated PFCB aryl ether polymers were shown to be hydrophobic, but demonstrated complete wetting by hydrocarbon-based liquids. Upon the incorporation of F-POSS as a drop-in modifier at low weight percent loadings, the composite fluoropolymer spin cast films exhibited increased water in addition to hydrocarbon repellency as determined by contact angle analysis. Composite film surface characterization included SEM and AFM analyses which showed excellent F-POSS dispersion and increased surface roughness. Thermal properties of the blended films were analysed using DSC and this demonstrated that the incorporation of F-POSS did not affect the bulk matrix properties inherent of PFCB aryl ether polymers. This work demonstrated that optimized F-POSS PFCB aryl ether polymer blend formulations have potential use as hydro- and oleophobic materials for seals, fibers, and coatings.
MRSA Infection of Upper Respiratory Tract. (1994)

A 48-year-old male with no concurrent underlying illness had a severe upper respiratory infection due to methicillin-resistant Staphylococcus aureus (MRSA). The clinical course suggested that it was a community-acquired MRSA infection. He also developed purpura of the legs and erythema of the hands. These skin eruptions were diagnosed as vasculitis allergica cutis accompanying by MRSA infection.From December 1992 to May 1993, 27 outpatients with acute suppurative pharyngitis and tonsillitis were tested for MRSA. It accounted for 6.7% of all bacterial strains isolated and for 13.3% of Staphylococcus aureus isolates. Community-acquired MRSA infection deserves greater attention, since morbidity tends to be greater in MRSA patients.
Wireless sensor network node deployment based on multi-objective immune algorithm (2018)

Wireless sensor network (WSN) is made up of a large number of low-cost wireless sensor nodes, which can collect all types of data in its lifetime. Node deployment of WSN is a NP complete problem, and it can significantly influence the network coverage and energy consumption in WSN. In this paper, we try to exploit multi-objective immune algorithm to solve the node deployment problem in WSN. The proposed node deployment algorithm aims to maximise the degree of network coverage and minimise the energy consumption. In the proposed multi-objective immune algorithm, each antibody refers to a candidate solution in node deployment process, and the antibodies are randomly initialised with a specific range. In addition, we design a fitness function by two ranking modes. Finally, experiments are conducted to test the performance of the proposed algorithm. Particularly, we use some typical performance metric in this experiment, such as coverage degree, energy consumption, and maximum moved distance. Experimental results demonstrate that the proposed can both enhance the network coverage degree and reduce the energy consumption by minimising the moved distance.
Multicenter Phase II Trial of Temozolomide in Patients With Anaplastic Astrocytoma or Anaplastic Oligoastrocytoma at First Relapse (1999)

Temozolomide demonstrated good single-agent activity, an acceptable safety profile, and documented HQL benefits in patients with recurrent AA.
An evolutionary algorithm approach to poetry generation (2004)

Poetry is a unique artifact of the human language faculty, with its defining feature being a strong unity between content and form. Contrary to the opinion that the automatic generation of poetry is a relatively easy task, we argue that it is in fact an extremely difficult task that requires intelligence, world and linguistic knowledge, and creativity.
ContEst: estimating cross-contamination of human samples in next-generation sequencing data (2011)

Supplementary data is available at Bioinformatics online.