gebhart/patrepeval huggingface dataset:
Strategies for Prokaryotic Expression of Eukaryotic Membrane Proteins (2001)

High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.
Towards high-throughput genotyping of SNPs by dynamic allele-specific hybridization (2001)

Analysis of single nucleotide polymorphisms (SNPs)--the most common form of variation in the human genome--has become a popular strategy for discovering genes involved in complex diseases such as Alzheimer's disease, obesity and diabetes. It is also widely anticipated that SNPs will play a major role in pharmacogenomics, where the identification of variations in specific genes relevant to drug efficacy, toxicity and metabolism will help to establish optimal therapeutic strategies for individual patients. Reflecting these expectations, many new SNP-related technologies have appeared over the past few years, each with unique advantages, but all with the common goal of simplifying and expediting SNP analysis. We recently introduced a technique termed dynamic allele-specific hybridization (DASH), a convenient method for SNP (and insertion-deletion) genotyping, which is highly applicable to both basic research and clinical diagnostics. Commercial DASH devices are now available, making the technology affordably accessible for all laboratories.
Unusual Magnesium Crystalline Nanoblades Grown by Oblique Angle Vapor Deposition (2007)

We observed the growth of unusual Mg nanoblades by oblique angle deposition. Although the vapor flux is obliquely incident, these nanoblades stand vertically on the substrates. The thickness of the Mg nanoblades along the incident vapor direction is reduced to approximately 15 nm to -30 nm at a vapor incident angle approximately 75 degrees, while the width perpendicular to the incident vapor direction is as wide as a few hundred nm. In addition to the anisotropic blade morphology, a (1010) [0001] biaxial (II-O) texture was observed using in situ reflection high energy electron diffraction (RHEED). The tilt angles of the texture axis and the nanoblades are correlated with the high surface diffusion on the (0001) surface along the [2130] direction. We also propose that the observed very thin thickness of the nanoblade along the vapor flux direction is due to the appearance of the surface steps parallel to the [0110] direction and the low surface diffusion on the top surface of the nanoblades.
Renin inhibitors as novel treatments for cardiovascular disease (2003)

AbstractThe renin–angiotensin system (RAS) plays an important role in the development of cardiovascular diseases such as hypertension and heart failure. Inhibitors of renin (EC 3.4.99.19) block the RAS at its first and rate-limiting step and may therefore offer major potential benefits in blood pressure control. Despite intensive research, however, previous attempts to develop clinically useful renin inhibitors have failed. This review discusses recent patent applications for the development of two novel classes of non-peptide renin inhibitors: 3-alkoxy-4-aryl-piperidines, such as Ro 66-1132 (Hoffmann-La Roche), and alkanecarboxamides, such as aliskiren (SPP-100; Novartis). Both classes are effective in animal models, while results from studies in humans suggest that aliskiren may represent the first in a new class of orally-effective renin inhibitors. Patents claiming the use of renin inhibitors in combination therapy are also discussed.Keywords4-aryl-piperidinealiskirenangiotensinantihypertensiveaspartyl proteasehypertensionleft ventricular dysfunctionrenal blood flowreninangiotensin systemrenin inhibitorsynthetic inhibitortransition-state peptidomimetic
Cost-Effectiveness of Coronary Artery Bypass Grafts Versus Percutaneous Coronary Intervention for Revascularization of High-Risk Patients (2006)

PCI was less costly and at least as effective for the urgent revascularization of medically refractory, high-risk patients over 5 years.
Sense & response service architecture (SARESA) (2005)

The dynamic business environment of many organizations require massive monitoring of their processes in real-time in order to proactively respond to exceptional situations and to take advantage of time-sensitive business opportunities. The ability to sense and interpret events about a changing business environment requires an event-driven IT infrastructure for pwerforming fast and well-informed decisions and putting them into action. However, traditional Business Intelligence (BI) and Data Warehousing technologies do not directly address time sensitive monitoring and analytical requirements. We introduce an enhanced BI architecture that covers the complete process to sense, interpret, predict, automate and respond to business environments and thereby aims to decrease the reaction time needed for business decisions. We propose an event-driven IT infrastructure to operate BI applications which enable real-time analytics across corporate business processes, notifies the business of actionable recommendations or automatically triggers business operations, and effectively closing the gap between Business Intelligence systems and business processes. A scenario from the area of mobile phone fraud detection was chosen for building a prototype that illustrates the proposed approach by using current available IT technologies.
Combination Immunotherapy of B-Cell Non-Hodgkin’s Lymphoma with Rituximab and Interleukin-2 (2004)

The combination of IL-2 and rituximab is synergistic against B-NHL in the hu-PBL-SCID model. In the phase I trial, a sequential combination of rituximab and IL-2 was well tolerated and achieved biological end points. Responses were observed.
Anchoring of Nematic Liquid Crystals on Self-Assembled Monolayers Formed from Alkanethiols on Semitransparent Films of Gold (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnchoring of Nematic Liquid Crystals on Self-Assembled Monolayers Formed from Alkanethiols on Semitransparent Films of GoldRichard A. Drawhorn and Nicholas L. AbbottCite this: J. Phys. Chem. 1995, 99, 45, 16511–16515Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://doi.org/10.1021/j100045a004Request reuse permissionsArticle Views795Altmetric-Citations95LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alerts
Poly(methylphenylsilane) film as a hole transport layer in electroluminescent devices (1991)

Electroluminescent devices were fabricated using poly(methylphenylsilane) and 8-hydroxyquinoline aluminum complex as the hole transport layer and the emitting layer, respectively. The cell structure of glass substrate/indium-tin-oxide/hole transport layer/emitting layer/Mg/Ag was employed. Smooth hole injection from the electrode through the polymer layer and concomitant efficient electroluminescence from the emitting layer were observed. Green emission with a luminance of over 1000 cd/m2 was achieved at a current density of 100 mA/cm2.
Chondroitinase ABC affects the activity of intracellular enzymes in rabbit articular cartilage chondrocytes. (1995)

Chondroitinase ABC induced marked proteoglycan depletion, which was not associated with light microscopy structural damage. The mechanism(s) by which chondroitinase ABC produces intracellular effects are unknown.
Synthetic and natural opiates interact with P-glycoprotein in multidrug-resistant cells (1993)

The ability of P-glycoprotein (P-gp) to transport naturally occurring and synthetic opiate analgesics was investigated. Multidrug-resistant Chinese hamster ovary cells (B30) were found to accumulate significantly lower amounts of morphine than their drug-sensitive counterparts (B1). This decreased accumulation was reversed upon depletion of cellular stores of ATP. In addition, morphine bound to plasma membranes from B30 cells in a specific, saturable fashion. Verapamil and vinblastine, compounds transported by P-gp, were able to increase accumulation and displace the binding of morphine in B30 cells. In turn, the synthetic opiates meperidine, pentazocine, and methadone were able to increase the accumulation of vinblastine in resistant cells. These compounds were also able to displace the specific binding of vinblastine and the photoaffinity labeling of P-gp in plasma membranes by the radioiodinated anthracycline, iodomycin. The implications of these findings in relation to the distribution, tolerance, and gastrointestinal side effects of opiates are discussed.
TRU-015, a novel CD20-directed biologic therapy, demonstrates significant anti-tumor activity in human tumor xenograft models (2005)

2549 Background: Small Modular Immuno-Pharmaceuticals (SMIPs) represent a novel proprietary biologic compound class that retain Fc mediated effector functions and are smaller than monoclonal antibodies. TRU-015, a SMIP that binds to CD20, depletes B cells in vitro and in animal models. TRU-015 has potent ADCC and apoptotic activity in vitro. By design, TRU-015 has attenuated CDC activity. We have evaluated the activity of TRU-015 in established human tumor xenograft models in nude mice. Methods: Nude mice were injected sub cutaneously with either Ramos or Daudi tumor cells. When large palpable tumors (> 200 mm3) were evident, mice (N ≥ 8/group) were treated with TRU-015 or comparator molecules. Groups were evaluated for median survival time (MST), tumor volume and percentage of tumor-free animals. Results: In nude mice bearing Ramos tumors >200mm3, TRU-015 treated mice demonstrated a significant enhancement in their median survival time (MST) in comparison to control mice (MST 44.5 d vs 11 d, p < 0.0001). In a more demanding model (tumor size > 370 mm3), TRU-015 treated mice also demonstrated a significant improvement in their MST in comparison to control mice (64.5 d vs 11 d, p < 0.0001). In nude mice bearing Daudi tumors, TRU-015 treated mice also showed a significant improvement in MST (40.5 d) in comparison to both rituximab (18 d, p<0.02) and control mice (10d, p<0.02). In addition, TRU-015 treated mice had decreased mean tumor volumes and a greater percentage of complete tumor regressions as compared to the other treatment groups in these tumor models. Conclusions: TRU-015 is effective in treating large, established tumors in these human tumor xenograft models. Author Disclosure Employment or Leadership Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Trubion Trubion
Comparison between Physiologic and Erythromycin-Induced Interdigestive Motility (1995)

Erythromycin given in a low dose is very effective in inducing phase-III-like motility. The last part of duodenal phase III is characterized by retroperistalsis also when this motility phase is induced by erythromycin.
68Ga-DOTA-Tyr3-Octreotide PET in Neuroendocrine Tumors: Comparison with Somatostatin Receptor Scintigraphy and CT (2007)

(68)Ga-DOTA-TOC PET shows a significantly higher detection rate compared with conventional somatostatin receptor scintigraphy and diagnostic CT with clinical impact in a considerable number of patients.
Uptake of high-density lipoprotein-associated apoprotein A-I and cholesterol esters by 16 tissues of the rat in vivo and by adrenal cells and hepatocytes in vitro. (1985)

The uptake of high-density lipoprotein (HDL)-associated apolipoprotein A-I and cholesterol esters was estimated in 16 tissues of the rat using rat HDL doubly labeled with nondegradable tracers; covalently attached 125I-tyramine-cellobiose traced apo-A-I, and [3H]cholesteryl linoleyl ether traced cholesterol esters. Both labels remained associated with the HDL fraction in the plasma, adequately traced their unlabeled counterparts, and were well trapped at their sites of uptake. Cholesteryl ether was taken up at a greater fractional rate than apo-A-I by adrenal, ovary, and liver: 7-fold, 4-fold, and 2-fold greater, respectively. The rates of uptake of cholesteryl ether and apo-A-I were about equal in the other tissues (except kidney). The disproportionate uptake of HDL cholesteryl ether relative to HDL apo-A-I was also observed in primary cultures of rat adrenal cells and hepatocytes. Uptake of both moieties in both cell types showed saturability. Both the absolute rate of uptake of [3H]cholesteryl ether and the ratio of ether uptake to apo-A-I uptake were greater in adrenal cells than in hepatocytes, consonant with the in vivo observations. Very similar results were obtained using HDL biologically labeled with [3H]cholesterol esters. The disproportionate uptake of [3H]cholesteryl ether was not significantly decreased by depletion of apo-E from the HDL nor by reductive methylation of the apo-E to block its recognition by receptors. However, apo-A-I uptake was decreased, suggesting that apo-E mediates the uptake of particles containing apo-A-I but does not contribute to the disproportionate uptake of [3H]cholesteryl ether.
Early Facial Expression Recognition Using Hidden Markov Models (2014)

Although it is often necessary to recognize users' expressions as soon as possible after it starts and before it ends in many applications, few methods have been proposed explicitly for early facial expression recognition. In this paper, we propose an early facial expression recognition method by using Hidden Markov Model. The relative displacement of the feature points between the current frame and the neutral frame are extracted as the facial features. During training, an iterative algorithm is introduced to find a classification entropy threshold and model parameters of early HMM. During testing, an image sequence is assigned an expression category when the entropy of the expression likelihood obtained from early HMMs is below the threshold by gradually increasing sequence length. Experimental results on CK+ and MMI databases show the effectiveness of our approach.
Fcγ receptor transmembrane domains: role in cell surface expression, γ chain interaction, and phagocytosis (2003)

We constructed chimeric receptors to dissect the role of the transmembrane (TM) domain in cell surface expression of and phagocytosis by the gamma chain-dependent Fcgamma receptors FcgammaRIIIA and FcgammaRI. FcgammaR chimeras containing the TM and cytoplasmic (CY) domains of the gamma chain were expressed on the cell surface and mediated an efficient phagocytic signal. In contrast, chimeras containing the FcgammaRIIIA TM were poorly expressed. Receptors containing the FcgammaRI TM and the gamma chain CY but lacking the gamma chain TM also were expressed efficiently and mediated phagocytosis, suggesting that a gamma chain dimer induced by the gamma chain TM is not required for efficient phagocytosis. Cotransfection of FcgammaRI or FcgammaRIIIA with the chimera CD8-gamma-gamma (EC-TM-CY) resulted in FcgammaR cell surface expression and phagocytosis, whereas CD8-CD8-gamma, whose TM does not associate with FcgammaR, allowed cell surface expression of (but not phagocytosis by) FcgammaRI. CD8-CD8-gamma also did not allow surface expression of FcgammaRIIIA. Exchanging FcgammaRI and CD8 TMs indicated that the C-terminal 11 amino acids of the FcgammaRI TM are essential for association of FcgammaRI with the gamma chain and phagocytosis. The data indicate that specific sequences in the FcgammaRIIIA and FcgammaRI TMs govern their different interactions with the gamma chain in cell surface expression and phagocytosis and that gamma chain TM sequences are not required for gamma chain-mediated phagocytosis. The data identify a specific region of the FcgammaRI TM and its asparagine as important for FcgammaRI cell surface expression in the absence of the gamma chain and for distinguishing the FcgammaRI and FcgammaRIIIA phenotypes.
Review article: mode of action and delivery of 5‐aminosalicylic acid – new evidence (2006)

The effectiveness of sulfasalazine depends on the splitting of the diazo bond in the molecule by the action of bacteria in the large bowel, releasing the pharmacologically active moiety, 5-aminosalicylic acid. The development of pH-dependent, delayed-release formulations of 5-aminosalicylic acid abolished the toxicity associated with the sulfapyridine part of sulfasalazine. 5-aminosalicylic acid is now believed to act by activating a class of nuclear receptors involved in the control of inflammation, cell proliferation, apoptosis and metabolic function, the gamma form of peroxisome proliferator-activated receptors. These receptors are expressed at particularly high levels in colon epithelial cells, where their expression appears to be at least in part stimulated by gut bacteria. Other drugs known to act via peroxisome proliferator-activated receptor-gamma, such as rosiglitazone and the selective peroxisome proliferator-activated receptor-gamma ligand GW1929, can be displaced from their binding sites on the peroxisome proliferator-activated receptor-gamma molecule by 5-aminosalicylic acid at concentrations of 5-aminosalicylic acid that correspond with the concentrations found in the lumen of ulcerative colitis patients taking oral mesalazine. Genetically engineered heterozygous knockout mice (peroxisome proliferator-activated receptor-gamma+/-) are particularly susceptible to colonic inflammation, and inflammation is more severe in these mice, in response to chemicals that induce experimental colonic ulcers. In these experimental models, 5-aminosalicylic acid is ineffective in peroxisome proliferator-activated receptor-gamma+/- mice. This new insight provides a mechanistic foundation for the possibility that long-term treatment with 5-aminosalicylic acid can reduce the risk of colorectal cancer in patients with ulcerative colitis.
Immunochemical Studies of Diphtherial Toxin and Related Nontoxic Mutant Proteins (1980)

Competitive binding radioimmunoassays were used to analyze the immunochemistry of diphtherial toxin. Rabbit antisera obtained by immunization with formolized toxoid or fragment A were used to characterize purified toxin, toxoid, fragment A, and related nontoxic mutant proteins. Antitoxoid serum had a high titer of neutralizing activity. Most of the antibodies in antitoxoid bound to toxin but not to fragment A. The anti-fragment A antibodies that were present in antitoxoid recognized determinants of fragment A that were exposed on unnicked toxin. Formaldehyde treatment partially destroyed antibody-binding sites associated with the A and B domains of toxin. Anti-fragment A serum had a low titer of neutralizing activity. The specificities of the anti-fragment A antibodies in antitoxoid and anti-fragment A sera were different. Approximately half of the anti-fragment A antibodies in anti-fragment A serum recognized determinants of fragment A that were masked in toxin. Per unit of fragment A-binding activity, anti-fragment A serum was significantly more potent than antitoxoid serum as an inhibitor of the enzymatic activity of fragment A. By analyzing the antigenic structure of several nontoxic mutant proteins (cross-reacting materials) that cross-react with toxin, we distinguished three different subgroups of antigenic determinants associated with the B domain of toxin. Furthermore, the exposed antigenic determinants of the A domain of toxin were separated into two subgroups, both of which were distinct from the masked determinants of the A domain. The radioimmunoassays described here provide rapid, sensitive, quantitative, and versatile methods for immunochemical characterization of toxin or related cross-reacting proteins encoded by corynebacteriophages.
Requirement for Tec Kinases Rlk and Itk in T Cell Receptor Signaling and Immunity (1999)

T cell receptor (TCR) signaling requires activation of Zap-70 and Src family tyrosine kinases, but requirements for other tyrosine kinases are less clear. Combined deletion in mice of two Tec kinases, Rlk and Itk, caused marked defects in TCR responses including proliferation, cytokine production, and apoptosis in vitro and adaptive immune responses to Toxoplasma gondii in vivo. Molecular events immediately downstream from the TCR were intact in rlk-/-itk-/- cells, but intermediate events including inositol trisphosphate production, calcium mobilization, and mitogen-activated protein kinase activation were impaired, establishing Tec kinases as critical regulators of TCR signaling required for phospholipase C-gamma activation.
A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety (1975)

1. 4-(N-2-Aminoethyl2′-pyridyl disulphide)-7-nitrobenzo-2-oxa-1,3-diazole (compound I) was synthesized and evaluated as a fluorescent labelling reagent for thiol groups. 2. The design of compound (I) as one example of a general type of reporter group delivery reagent (2-pyridyl-S-S-X, where X contains an environmentally sensitive spectroscopic probe) is discussed. 3. The electronic absorption spectrum of compound (I) was determined over a wide range of pH and the spectral changes that accompany its reaction with low-molecular-weight thiols, e.g. L-cysteine, and with papain (EC 3.4.22.2) and bovine serum albumin are discussed. 4. A new value of epsilon343 for 2-thiopyridone (Py-2-SH) was determined as 8.08 × 10(3) +/- 0.08 × 10(3)M-1-cm-1. 5. Spectral analysis of the reactions of compound (I) with L-cysteine and with papain (in the pH range 3.5-8.0) showed that even under equimolar conditions the reaction (thiol-disulphide interchange to release Py-2-SH) is essentially stoicheimoetric and probably proceeds by specific attack at the sulphur atom distal from the pyridyl ring of compound (I). 6. The fluorescence-emission spectra of compound (I) and of the products of its reaction with papain and with ficin (EC 3.4.22.3) were determined. Compound (I) is highly fluorescent in aqueous solution. Excitation within the intense visible absorption band (lambda max. 481 nm, epsilon max. 2.52 × 10(4)M-1-cm-1) provides green fluorescence with an emission maximum at 540 nm. Both papain and ficin labelled by reaction with compound (I) are characterized by fluorescence-emission maxima (535 nm and 530 nm respectively) of even higher intensity. The fluorescence emission of the product of the reaction of papain with compound (I) was shown to be 25 times more intense than that of the product of the reaction of papain with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (Nbd chloride). 7. The second-order rate constants (k2) for the reactions of compound (I) and of Nbd chloride with GSH, papain, albumin, ficin, 2-benzimidazolylmethanethiol and 2-benzimidazolylethanethiol were determined at 25.0 degrees C and various pH values. At pH4 the values of k2(compound I)/k2(Nbd chloride) are: GSH, 288; albumin, 36; papain 3 × 10(3); ficin, 3 × 10(4). 8. The pH-k2 profiles for the reactions of compound (I) and of Nbd chloride with the two 2-benzimidazolylalkanethiols were determined. Of the four profiles only that for the reaction of compound (I) with 2-benzimidazolylmethanethiol is characterized by a striking rate maximum in acidic media.