gebhart/patrepeval huggingface dataset:
Bacteria-infected fibroblasts have enhanced susceptibility to the cytotoxic action of tumor necrosis factor. (1990)

Abstract The susceptibility of bacteria-infected fibroblasts to the cytotoxic action of tumor necrosis factor was investigated. L cells infected with Shigella flexneri, Salmonella typhimurium, or Listeria monocytogenes, had an enhanced susceptibility to the cytotoxic activity of TNF-alpha. This enhanced susceptibility was dependent upon the challenge dose of bacteria, the concentration of TNF, and upon the exposure time of bacteria-infected cells to TNF. L cells infected with S. flexneri were susceptible to the cytotoxic action of TNF at 2 to 6 h after bacterial infection. In contrast, L cells infected with S. typhimurium or L. monocytogenes did not show enhanced susceptibility to TNF until 14 h postbacterial infection and exposure to TNF. Enhanced susceptibility to TNF was dependent on bacterial invasion because fibroblasts pretreated with a noninvasive isogenic variant of S. flexneri, UV-treated invasive bacteria, bacterial cultural supernatant, or bacteria LPS were no more susceptible to TNF than untreated cells. Enhanced susceptibility to TNF by bacteria-infected cells was not unique to L cells. Mouse embryo fibroblasts and HeLa cells also showed similar reactivities after bacteria infection. Bacteria-infected cells were greatly suppressed in host cell protein synthesis that may play an important role in their enhanced susceptibility to TNF. These results suggest that an important role of TNF in host defense against bacterial infections is its cytotoxic activity against bacteria-infected cells.
The cochlear frequency map for the cat: Labeling auditory-nerve fibers of known characteristic frequency (1982)

Iontophoresis of horseradish peroxidase was used to label single auditory nerve fibers after determination of threshold tuning curves and rates of spontaneous discharge. The relation between characteristic frequency (CF) and cochlear longitudinal location is reconstructed from 52 labeled neurons in 16 cochleas. The length of the organ of Corti allotted to an octave of stimulus frequency increases steadily from low to high frequencies. Thus there is not a simple linear-distance-to-log frequency conversion. When comparing cochleas of different total length, the best predictor of CF at a given location is the distance from base or apex expressed as a percentage of the total length. The cochlear frequency map derived from these single-neuron labeling experiments is compared to maps derived by a number of different physiological and psychophysical techniques, and the significance of the similarities and differences is discussed.
Interaction between Presenilin 1 and Ubiquilin 1 as Detected by Fluorescence Lifetime Imaging Microscopy and a High-throughput Fluorescent Plate Reader (2006)

Presenilin 1 (PS1) in its active heterodimeric form is the catalytic center of the gamma-secretase complex, an enzymatic activity that cleaves amyloid precursor protein (APP) to produce amyloid beta (Abeta). Ubiquilin 1 is a recently described PS1 interacting protein, the overexpression of which increases PS1 holoprotein levels and leads to reduced levels of functionally active PS1 heterodimer. In addition, it has been suggested that splice variants of the UBQLN1 gene are associated with an increased risk of developing Alzheimer disease (AD). However, it is still unclear whether PS1 and ubiquilin 1 interact when expressed at endogenous levels under normal physiological conditions. Here, we employ three novel fluorescence resonance energy transfer-based techniques to investigate the interaction between PS1 and ubiquilin 1 in intact cells. We consistently find that the ubiquilin 1 N terminus is in close proximity to several epitopes on PS1. We show that ubiquilin 1 interacts both with PS1 holoprotein and heterodimer and that the interaction between PS1 and ubiquilin 1 takes place near the cell surface. Furthermore, we show that the PS1-ubiquilin 1 interaction can be detected between endogenous proteins in primary neurons in vitro as well as in brain tissue of healthy controls and Alzheimer disease patients, providing evidence of its physiological relevance.
Optical crossconnect/ADM based networks (2002)

Optical crossconnect and add drop multiplexer (ADM) are key components for realizing future high-capacity and flexible communication networks, such as the "optical fiber freeway". We have proposed and demonstrated the optical crossconnect and ADM using photonic SD and WD switching technologies. With a future study on an all optical network management scheme, a high capacity and flexible optical network would be realized.
Effect of heat treatment on the performance of carbon-supported transition-metal chelates in the electrochemical reduction of oxygen (1981)

A study has been made of the changes occurring in carbon-supported metalloporphyrins and metallo-phthalocyanines during heat treatment, which improves both the stability and the activity of these materials in the electrochemical reduction of oxygen in acid electrolyte. A variety of physical techniques was applied, including X-ray photoelectron spectroscopy (X.p.s.), Fourier-transform infrared spectroscopy, electron spin resonance and Mössbauer spectrometry.During the heat treatment a bond can be formed between the carbon surface and both the central metal ion and the macrocyclic ligand, which thereby loses all its peripheral substituents while retaining its inner part. In some cases some of the chelate molecules are destroyed. The active sites formed in the more active catalysts consist of an isolated metal ion surrounded by the thermally modified ligand. A relation between redox potential and O2-reduction activity of the same form as that for untreated catalysts can be used to explain the observed enhancements in activity.
Efflux of choline and glycine betaine from osmoregulating cells of Escherichia coli (1992)

We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. coli has efflux systems for betaine and choline which are independent of the uptake systems for these metabolites. The ProU system of E. coli, but not that of Salmonella typhimurium, can mediate low-affinity choline uptake.
DC and AC performance analysis of 25 nm symmetric/asymmetric double-gate, back-gate and bulk CMOS (2002)

In this paper, the performance of 25 nm double-gate, back-gate and super-halo CMOS devices has been analyzed, including the self-consistent 2D quantization effect. The drive current is enhanced by the gate-to-body coupling effect for double-gate with ultra-thin body. The channel quantization effect can substantially degrade the drive current for asymmetric double-gate, back-gate, and bulk CMOS ICs. It is demonstrated that the exceptional SCE immunity in SDG offers substantial performance leverage over conventional MOSFET structures.
Effect of Circulating Peptide YY on Gallbladder Emptying in Humans (2001)

Circulating PYY suppresses the cephalic phase of postprandial gallbladder emptying, but not meal stimulated maximum emptying. The results support the hypothesis that the effect of PYY on gallbladder emptying is mediated by vagal-dependent rather than cholecystokinin-dependent pathways.
Functional Imaging of Brown Fat in Mice with <sup>18</sup>F-FDG micro-PET/CT (2012)

Brown adipose tissue (BAT) differs from white adipose tissue (WAT) by its discrete location and a brown-red color due to rich vascularization and high density of mitochondria. BAT plays a major role in energy expenditure and non-shivering thermogenesis in newborn mammals as well as the adults (1). BAT-mediated thermogenesis is highly regulated by the sympathetic nervous system, predominantly via β adrenergic receptor (2, 3). Recent studies have shown that BAT activities in human adults are negatively correlated with body mass index (BMI) and other diabetic parameters (4-6). BAT has thus been proposed as a potential target for anti-obesity/anti-diabetes therapy focusing on modulation of energy balance (6-8). While several cold challenge-based positron emission tomography (PET) methods are established for detecting human BAT (9-13), there is essentially no standardized protocol for imaging and quantification of BAT in small animal models such as mice. Here we describe a robust PET/CT imaging method for functional assessment of BAT in mice. Briefly, adult C57BL/6J mice were cold treated under fasting conditions for a duration of 4 hours before they received one dose of (18)F-Fluorodeoxyglucose (FDG). The mice were remained in the cold for one additional hour post FDG injection, and then scanned with a small animal-dedicated micro-PET/CT system. The acquired PET images were co-registered with the CT images for anatomical references and analyzed for FDG uptake in the interscapular BAT area to present BAT activity. This standardized cold-treatment and imaging protocol has been validated through testing BAT activities during pharmacological interventions, for example, the suppressed BAT activation by the treatment of β-adrenoceptor antagonist propranolol (14, 15), or the enhanced BAT activation by β3 agonist BRL37344 (16). The method described here can be applied to screen for drugs/compounds that modulate BAT activity, or to identify genes/pathways that are involved in BAT development and regulation in various preclinical and basic studies.
Infrared studies of apatites. II. Preparation of normal and isotopically substituted calcium, strontium, and barium hydroxyapatites and spectra-structure-composition correlations (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInfrared studies of apatites. II. Preparation of normal and isotopically substituted calcium, strontium, and barium hydroxyapatites and spectra-structure-composition correlationsB. O. FowlerCite this: Inorg. Chem. 1974, 13, 1, 207–214Publication Date (Print):January 1, 1974Publication History Published online1 May 2002Published inissue 1 January 1974https://pubs.acs.org/doi/10.1021/ic50131a040https://doi.org/10.1021/ic50131a040research-articleACS PublicationsRequest reuse permissionsArticle Views743Altmetric-Citations161LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Adsorption of Cyanine Dyes on Gold Nanoparticles and Formation of J-Aggregates in the Nanoparticle Assembly (2006)

This paper describes the results of an investigation of the interparticle interactions and reactivities in the assembly of gold nanoparticles mediated by cyanine dyes. The combination of the positively charged indolenine cyanine dyes and the negatively charged gold nanoparticles is shown to form a J-aggregate bridged assembly of nanoparticles, in addition to hydrophobic interparticle and electrostatic dye-particle interactions. Such interparticle interactions and reactivities are studied by probing the absorption of J-aggregates and fluorescence from the dyes and the surface plasmon resonance absorption from the nanoparticles. The J-aggregation of the dyes adsorbed on the nanoparticles is shown to play an important role in the assembly of nanoparticles. The spectral evolution of the J-band of the dyes and the surface plasmon resonance band of the nanoparticles was found to be sensitive to the nature of the charge and the structure of the dyes. The fluorescence quenching for the dyes was shown to be quantitatively related to the surface coverage of the dyes on the nanocrystal surfaces. These findings have provided important information for assessing a two-step process involving a rapid adsorption of the dyes on the nanoparticles and a subsequent assembly of the nanoparticles involving a combination of interparticle J-aggregation and hydrophobic interactions of the adsorbed dyes. The results are discussed in terms of the structural effects of the dyes, and the interparticle molecular interactions and reactivities, which provide important physical and chemical insights into the design of dye-nanoparticle structured functional nanomaterials.
Catalytic Asymmetric Activation of a CH Bond Adjacent to a Nitrogen Atom: A Versatile Approach to Optically Active α‐Alkyl α‐Amino Acids and C1‐Alkylated Tetrahydroisoquinoline Derivatives (2011)

Simple and efficient: A one-pot oxidative and catalytic enantioselective alkylation of α-CH bonds adjacent to a nitrogen atom was realized for the first time. This novel strategy provides a simple, efficient, and environmentally friendly access to diverse optically active α-alkyl α-amino acid and C1-alkylated tetrahydroisoquinoline derivatives.
Investigation of chloroquine intake in Biombo region, Guinea Bissau. (1994)

The use of chloroquine (CQ), during the dry season was determined for 236 patients from 5 villages in Biombo, Republic of Guinea Bissau, West Africa. The antimalarial drug was measured in whole blood (dried samples) using High Performance Liquid Chromatography (HPLC) and in urine samples by Enzyme Linked Immunosorbent Assay (ELISA). The results showed that CQ consumption is low.
Molecular Weight and Chemical Structure as Factors in the Biliary Excretion of Sulphonamides in the Rat (1972)

1. Biliary excretion in biliary-cannulated female rats of 23 derivatives of sulphanilamide, in which the N4-position is substituted with various carboxyacyl groups and the N1-position with acetyl or 2-thiazole, has been studied.2. Six compounds having molecular weights in the range 172–314 were poorly excreted in the bile (<5% of dose) but seventeen others (mol. wt. range 319–719) were eliminated in the bile to an appreciable extent (7–74%). For the compounds studied there appears to be a threshold value for the mol. wt. (320–370) at and above which biliary excretion becomes appreciable (>5–10% of dose).3. Above the threshold mol. wt., biliary excretion is high (20–70% of dose) but there is no direct relationship between the two, as the extent of biliary excretion is not directly proportional to mol. wt.4. There is no direct relationship between the extent of biliary excretion and the relative lipid solubilities as measured by distribution ratios between buffer pH 7.4 and organic solvents. Nevertheless there is a general trend for compounds which are extensively eliminated in the bile to be more lipophilic than those that are not excreted in the bile.5. Above the threshold mol. wt. marked variations in the extent of biliary excretion of compounds having similar mol. wt. can occur. These variations can be attributed to the influence of a ‘structural factor’ in biliary excretion. This factor could affect the lipid solubility or the shape of the molecule.
An Orally Administered Multitarget Tyrosine Kinase Inhibitor, SU11248, Is a Novel Potent Inhibitor of Thyroid Oncogenic RET/Papillary Thyroid Cancer Kinases (2006)

SU11248 is a highly effective tyrosine kinase inhibitor of the RET/PTC oncogenic kinase.
Immunomodulatory Activities of Small Host Defense Peptides (2005)

Recent studies have demonstrated that in addition to their antimicrobial activity, cationic host defense peptides, like the human cathelicidin LL-37, perform many activities relating to innate immunity, including the induction or modulation of chemokine and cytokine production, alteration of gene expression in host cells, and inhibition of proinflammatory responses of host cells to bacterial components such as lipopolysaccharide (LPS) in vitro and in vivo. To investigate if these properties are shared by smaller peptides, two cathelicidin peptides derived from bovine neutrophils, the 13-mer indolicidin and Bac2A, a linear 12-amino-acid derivative of bactenecin, were compared to the 37-amino-acid peptide LL-37. Indolicidin, like LL-37, inhibited LPS-induced tumor necrosis factor alpha (TNF-alpha) secretion, even when added up to an hour after the addition of Escherichia coli O111:B4 LPS to the human macrophage/monocyte-like THP-1 cell line. In contrast, Bac2A demonstrated no significant antiendotoxin activity. At low concentrations, indolicidin and LL-37 acted synergistically to suppress LPS-induced production of TNF-alpha. Indolicidin was analogous to LL-37 in its ability to induce the production of the chemokine interleukin-8 (IL-8) in a human bronchial cell line, 16HBE14o(-), but it was unable to induce production of IL-8 in THP-1 cells. In contrast, Bac2A was unable to induce IL-8 in either cell type. Conversely, Bac2A was chemotactic for THP-1 cells at concentrations between 10 and 100 mug/ml, while indolicidin and LL-37 were not chemotactic at these concentrations for THP-1 cells. This indicates that in addition to the potential for direct microbicidal activity, cationic host defense peptides may have diverse and complementary abilities to modulate the innate immune response.
Mass spectrometry as a novel method for detection of podocyturia in pre-eclampsia (2012)

The LC-MS/MS method is a reliable technology for the identification of urinary podocytes, based on the presence of podocyte-specific proteins in the urine.
Approximating and intersecting surfaces from points (2003)

Point sets become an increasingly popular shape representation. Most shape processing and rendering tasks require the approximation of a continuous surface from the point data. We present a surface approximation that is motivated by an efficient iterative ray intersection computation. On each point on a ray, a local normal direction is estimated as the direction of smallest weighted co-variances of the points. The normal direction is used to build a local polynomial approximation to the surface, which is then intersected with the ray. The distance to the polynomials essentially defines a distance field, whose zero-set is computed by repeated ray intersection. Requiring the distance field to be smooth leads to an intuitive and natural sampling criterion, namely, that normals derived from the weighted co-variances are well defined in a tubular neighborhood of the surface. For certain, well-chosen weight functions we can show that well-sampled surfaces lead to smooth distance fields with non-zero gradients and, thus, the surface is a continuously differentiable manifold. We detail spatial data structures and efficient algorithms to compute ray-surface intersections for fast ray casting and ray tracing of the surface.
Crystal Structure of the γ-Glutamyltranspeptidase Precursor Protein from Escherichia coli (2006)

Gamma-glutamyltranspeptidase (GGT) is an extracellular enzyme that plays a key role in glutathione metabolism. The mature GGT is a heterodimer consisting of L- and S-subunits that is generated by posttranslational cleavage of the peptide bond between Gln-390 and Thr-391 in the precursor protein. Thr-391, which becomes the N-terminal residue of the S-subunit, acts as the active residue in the catalytic reaction. The crystal structure of a mutant GGT, T391A, that is unable to undergo autocatalytic processing, has been determined at 2.55-A resolution. Structural comparison of the precursor protein and mature GGT demonstrates that the structures of the core regions in the two proteins are unchanged, but marked differences are found near the active site. In particular, in the precursor, the segment corresponding to the C-terminal region of the L-subunit occupies the site where the loop (residues 438-449) forms the lid of the gamma-glutamyl group-binding pocket in the mature GGT. This result demonstrates that, upon cleavage of the N-terminal peptide bond of Thr-391, the newly produced C terminus (residues 375-390) flips out, allowing the 438-449 segment to form the gamma-glutamyl group-binding pocket. The electron density map for the T391A protein also identified a water molecule near the carbonyl carbon atom of Gln-390. The spatial arrangement around the water and Thr-391 relative to the scissile peptide bond appears suitable for the initiation of autocatalytic processing, as in other members of the N-terminal nucleophile hydrolase superfamily.
Barite-Sag Management: Challenges, Strategies, Opportunities (2004)

Abstract Barite sag continues to be a recurring, potentially serious problem on many directional wells. Despite concerted efforts by the drilling industry and early progress, recent continued improvements in sag mitigation have been limited. Sag is a particular problem on HTHP wells and in deepwater wells where ECD management is required. These wells pose difficult drilling conditions where drilling practices may offset sag-management advancements. The sag "magic bullet" has thus far been elusive. This is understandable since sag is affected by many parameters and their interactions are difficult to quantify. While the importance of mud rheology is well known, attempts to find the key rheological parameter have not been completely successful. Furthermore, lack of industry standards to measure and report barite sag has limited the availability of usable field data. Sound engineering strategies and guidelines have helped, but clearly more developments are needed. The primary objectives of this paper are to (a) examine key barite-sag challenges, (b) characterize current best practices, and (c) discuss strategies, opportunities, and active programs for step improvements. Recent barite-sag case histories from the Gulf of Mexico, West Africa, and Atlantic Canada are included to set the proper perspectives.