gebhart/patrepeval huggingface dataset:
P16INK4A as an adjunct test in liquid-based cytology. (2003)

ThinPrep cases in the equivocal cytologic category with the corresponding tissue biopsy assayed for P16INK4A expression showed that there was utility for this type of testing. A larger series comparing corresponding ThinPrep and tissue biopsies will be undertaken. The role of HPV infection in these cases will also be explored.
Gardnerella vaginalis bacteremia: a review of thirty cases. (1984)

The authors documented thirty cases of bacteremia, all in gynecologic and obstetric patients, over a four-year period at a university hospital. Sixteen of the patients had polymicrobial infections. Most of the patients recovered completely whether or not antimicrobial therapy directed against Gardnerella vaginalis was given. G vaginalis bacteremia may occur more often than has been reported.
Shared Antigenic Epitopes and Pathobiological Functions of Anti-p185 Monoclonal Antibodies (1999)

We have studied two anti-p185 antibodies: the monoclonal antibody 7. 16.4 and rhuMAb 4D5, which were raised against the the ectodomain of rat (p185(neu)), and the human (p185(her2/neu)) homolog, respectively. Studies on the structure of these two antibodies indicate that they share structural similarity in the variable region, especially the CDR3 region, which determines the antibody-antigen interaction. Further studies by flow cytometry revealed that 7.16.4 can compete with rhuMAb4D5 for binding to the cell surface p185(her2/neu), suggesting that these two antibodies share an epitope on the p185 receptor. Furthermore, 7.16.4 can also inhibit proliferation and transformation caused by p185(her2/neu). Moreover the rhuMAb 4D5 binds to the rat p185(neu). With the observation that 7.16.4 positively stains human breast cancer tissues that overexpress p185(her2/neu), 7.16.4 may be useful for the pathological diagnosis and therapy of human tumors.
Probe Selection and Expression Index Computation of Affymetrix Exon Arrays (2006)

Our results demonstrate that probe selection improves gene expression estimates from exon arrays. The selected probes can be used in future analyses of other exon array datasets to compute gene expression indexes.
Synthesis and optical resolution of (R)- and (S)-trans-7-hydroxy-2-[N-propyl-N-(3'-iodo-2'-propenyl)amino]tetralin: a new D3 dopamine receptor ligand (1993)

An improved method for synthesis and resolution of (R,S)-trans-7-hydroxy-2-[N-propyl-N-(3'-iodo-2'-propenyl)amino]tetralin (trans-7-OH-PlPAT,5), a new D3 dopamine receptor ligand, was reported. Both isomers, (R)-(+)- and (S)-(-)-5, were prepared and characterized. HPLC retention times obtained on a chiral column for these isomers were consistent with those observed for [125I]-(R)-(+)- and (S)-(-)-5. Direct radioiodination of an optically resolved tin precursor, (R)-(+)-7, yielded the desired [125I](R)-(+)-5, which is a simpler method for synthesis of this ligand. Binding studies with membrane preparations containing D3 dopamine receptors expressed in Spodoptera frugiperda (Sf9) cells also suggested that the [125I](R)-(+)-5 is the active isomer (Kd = 0.05 nM). The schemes described may provide an efficient way for synthesizing a large quantity of this new D3 dopamine receptor ligand for in vivo behavior studies.
B cells can prime naive CD4+ T cells in vivo in the absence of other professional antigen‐presenting cells in a CD154‐CD40‐dependent manner (2005)

The role of B cells as APC is well established. However, their ability to prime naive T cells in vivo has been difficult to examine because of the presence of dendritic cells. The current studies were undertaken to examine this issue in a model of adoptive transfer of antigen-specific B cells and T cells into histoincompatible Rag2(-/-) mice. By means of this system, we were able to demonstrate that antigen-specific B cells are competent APC for naive CD4(+) T cells specific for the same antigen. In vivo antigen presentation resulted in expansion of both CD4(+) T cells and B cells. The antigen-presenting function of the transferred B cells was dependent on the CD154-CD40 interaction, as transfer of CD154-deficient antigen-specific CD4(+) T cells or CD40-deficient B cells failed to induce T and B cell expansion in response to immunization. These results indicate that antigen-specific B cells have the capacity to induce primary T cell responses in the absence of other competent APC.
Expression of Costimulatory Molecules (4-1BBL and Fas) and Major Histocompatibility Class I Chain-Related A (MICA) in Aortic Tissue with Takayasu’s Arteritis (2004)

To further investigate the immunological mechanisms involved, we analyzed the expression of costimulatory molecules in aortic tissue and their counterpart molecules on infiltrating cells of patients with Takayasu's arteritis. We also examined the expression of major histocompatibility complex (MHC) class I chain-related (MIC) A in aortic tissue, which is known to be induced by external stress, and its counterpart NKG2D receptors on infiltrating cells. Among these costimulatory molecules, strong expression of 4-1BBL and Fas was induced in the aortic tissue, and most of the infiltrating cells expressed 4-1BB and FasL, suggesting these pathways play critical roles in T-cell-mediated vascular injury. We also found that MICA was strongly induced in the aortic tissue and that at least part of the infiltrating cells expressed NKG2D receptors. Some infiltrating cells - but not vascular smooth muscle cells - seemed to have undergone apoptosis. Our findings strongly suggest that 4-1BB/4-1BBL and Fas/FasL pathways play important roles in vascular injury in Takayasu's arteritis. We assume that gammadelta T cells infiltrated aortic tissue recognizing MICA, resulting in the induction of MHC antigens and costimulatory molecules, and then alphabeta T-cells infiltrated recognizing some auto-antigens presented by MHC antigens, leading to chronic inflammation.
Interrelationships between lysine and α∈-diaminopimelic acid and their derivatives and analogues in mutants of Escherichia coli (1957)

Research Article| June 01 1957 Interrelationships between lysine and α∈-diaminopimelic acid and their derivatives and analogues in mutants of Escherichia coli Pauline Meadow; Pauline Meadow 1Department of Chemical Pathology, University College Hospital Medical School, London, W.C. 1 Search for other works by this author on: This Site PubMed Google Scholar D. S. Hoare; D. S. Hoare 1Department of Chemical Pathology, University College Hospital Medical School, London, W.C. 1 Search for other works by this author on: This Site PubMed Google Scholar Elizabeth Work Elizabeth Work 1Department of Chemical Pathology, University College Hospital Medical School, London, W.C. 1 Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1957) 66 (2): 270–282. https://doi.org/10.1042/bj0660270 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation Pauline Meadow, D. S. Hoare, Elizabeth Work; Interrelationships between lysine and α∈-diaminopimelic acid and their derivatives and analogues in mutants of Escherichia coli. Biochem J 1 June 1957; 66 (2): 270–282. doi: https://doi.org/10.1042/bj0660270 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1957 CAMBRIDGE UNIVERSITY PRESS1957 Article PDF first page preview Close Modal You do not currently have access to this content.
One-year treatment with mometasone furoate in chronic obstructive pulmonary disease (2008)

Many patients with chronic obstructive pulmonary disease (COPD) are treated with twice daily (BID) inhaled corticosteroids (ICS). This study evaluated whether daily PM mometasone furoate administered via a dry powder inhaler (MF-DPI) was equally effective compared to twice daily dosing.In a 52-week, randomized, double-blind, placebo-controlled study, 911 subjects with moderate-to-severe COPD managed without ICS received MF-DPI 800 microg QD PM, MF-DPI 400 microg BID, or placebo. The change from baseline in postbronchodilator forced expiratory volume in 1 second (FEV1), total COPD symptom scores, and health status as well as the percentage of subjects with a COPD exacerbation were assessed. Adverse events were recorded. Mometasone furoate administered via a dry powder inhaler 800 microg QD PM and 400 microg BID significantly increased postbronchodilator FEV1 from baseline (50 mL and 53 mL, respectively, versus a 19 mL decrease for placebo; P < 0.001). The percentage of subjects exacerbating was significantly lower in the pooled MF-DPI groups than in the placebo group (P = 0.043). Subjects receiving MF-DPI 400 microg BID reported a statistically significant (19%) reduction in COPD symptom scores compared with placebo (P < 0.001). Health status as measured with St. George's Respiratory Questionnaire (SGRQ) improved significantly in all domains (Total, Activity, Impacts, and Symptoms) in the pooled MF-DPI groups versus placebo (P < or = 0.031). MF-DPI treatment was well tolerated.Once-daily MF-DPI improved lung function and health status in subjects with moderate-to-severe COPD and was comparable to BID MF-DPI.
The LLNL (Lawrence Livermore National Laboratory) ICF (Inertial Confinement Fusion) Program: Progress toward ignition in the Laboratory (1990)

The Inertial Confinement Fusion (ICF) Program at the Lawrence Livermore National Laboratory (LLNL) has made substantial progress in target physics, target diagnostics, and laser science and technology. In each area, progress required the development of experimental techniques and computational modeling. The objectives of the target physics experiments in the Nova laser facility are to address and understand critical physics issues that determine the conditions required to achieve ignition and gain in an ICF capsule. The LLNL experimental program primarily addresses indirect-drive implosions, in which the capsule is driven by x rays produced by the interaction of the laser light with a high-Z plasma. Experiments address both the physics of generating the radiation environment in a laser-driven hohlraum and the physics associated with imploding ICF capsules to ignition and high-gain conditions in the absence of alpha deposition. Recent experiments and modeling have established much of the physics necessary to validate the basic concept of ignition and ICF target gain in the laboratory. The rapid progress made in the past several years, and in particular, recent results showing higher radiation drive temperatures and implosion velocities than previously obtained and assumed for high-gain target designs, has led LLNL to propose an upgrade of the Nova laser to 1.5 to 2 MJ (at 0.35 {mu}m) to demonstrate ignition and energy gains of 10 to 20 -- the Nova Upgrade.
Functional Expression in Lactobacillus plantarum of xylP Encoding the Isoprimeverose Transporter of Lactobacillus pentosus (1998)

The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter. Functional expression of the XylP transporter was shown by uptake of isoprimeverose in L. plantarum 80 cells, and this transport was driven by the proton motive force generated by malolactic fermentation. XylP was unable to catalyze transport of D-xylose.
Efficacy and Mechanism of Orthotic Devices to Unload Metatarsal Heads in People With Diabetes and a History of Plantar Ulcers (2006)

The TCI and the MP caused substantial and additive reductions of pressures under the metatarsal heads. The TCI reduces excessive pressures at the metatarsal heads by increasing the contact area of weight-bearing forces. Conversely, the MP acts by compressing the soft tissues proximal to the metatarsal heads and relieving compression at the metatarsal heads. These findings can assist in the design of effective orthotic devices to relieve excessive plantar stresses that contribute to skin breakdown and subsequent amputation in people with DM and peripheral neuropathy.
Antiinflammatory Triterpenoid Saponins from the Seeds of Aesculus chinensis (2004)

Phytochemical study of the ethanol extract of the seeds of Aesculus chinensis led to the isolation of a new triterpenoid saponin (6), together with five known triterpenoid saponins (1-5). The structure of the new compound was elucidated on the basis of spectral data to be 21,28-di-O-acetylprotoaescigenin-3-O-[beta-D-glucopyranosyl(1-2)][beta-D-glucopyranosyl(1-4)]-beta-D-glucopyranosiduronic acid (aesculiside A, 6). The antiinflammatory activities of the four main saponins (1-4) were compared with those of total saponin extracts, and single saponins showed more potent activity than total saponin extracts in mice.
A Direct Synthesis of Renewable Sulfonate‐Based Surfactants (2012)

Abstract Sulfonate‐based anionic surfactants were generated in 2–3 steps from fructose by conversion of fructose to a furan followed by generation of the sulfonate.
A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains (1987)

In this paper, we describe a 3.8-kb molecular construct that we have used to disrupt yeast genes. The construct consists of a functional yeast URA3 gene flanked by 1.1-kb direct repeats of a bacterial sequence. It is straightforward to insert the 3.8-kb segment into a cloned target gene of interest and then introduce the resulting disruption into the yeast genome by integrative transformation. An appropriate DNA fragment containing the disruption plus flanking homology can be obtained by restriction enzyme digestion. After introducing such fragments into yeast by transformation, stable integrants can be isolated by selection for Ura+. The important feature of this construct that makes it especially useful is that recombination between the flanking direct repeats occurs at a high frequency (10(-4)) in vegetatively grown cultures. After excision, only one copy of the repeat sequence remains behind. Thus in the resulting strain, the Ura+ selection can be used again, either to disrupt a second gene in similar fashion or for another purpose.
Role of the Thrombin Receptor's Cytoplasmic Tail in Intracellular Trafficking (1996)

The G protein-coupled thrombin receptor is activated by an irreversible proteolytic mechanism and, perhaps as a result, exhibits an unusual trafficking pattern in the cell. Naive receptors tonically cycle between the cell surface and a protected intracellular pool, whereas receptors cleaved and activated at the cell surface internalize and move to lysosomes. Toward understanding how these trafficking events are regulated, we examined a series of receptor mutants. A receptor with alanine substitutions at all potential phosphorylation sites in the cytoplasmic tail failed to display agonist-triggered internalization but, like wild type receptor, displayed robust signaling, tonic cycling, and localization to both the cell surface and an intracellular pool. A truncation mutant that lacked most of the cytoplasmic tail also signaled robustly, lacked phosphorylation, and was defective in agonist-triggered internalization. However, in contrast to the specific phosphorylation site mutant, the truncation mutant did not display tonic cycling and localized exclusively to the cell surface. An analysis of a series of truncation mutants localized residues important for receptor trafficking to a 10-amino acid stretch in its cytoplasmic tail. These data suggest that phosphorylation may trigger internalization of activated thrombin receptors but that a second phosphorylation-independent signal mediates tonic internalization of naive receptors. They further suggest that maintenance of the intracellular pool of naive thrombin receptors requires tonic receptor internalization.
Dengue and Yellow Fever — Challenges for the Development and Use of Vaccines (2007)

We currently have no vaccines to prevent dengue and there have been substantial recent problems with the yellow fever vaccine. Dr. Thomas Monath discusses the challenges in developing vaccines against these insect-borne viruses. Dr. Thomas Monath discusses the global threat represented by dengue and yellow fever and the challenges faced in the development and use of vaccines against them. Dr. Monath, an adjunct professor at the Harvard School of Public Health, Boston, spent many years studying, and developing vaccines against, dengue and yellow fever.
Descriptive Data Analysis of File Transfer Data (2014)

There are millions of files and multi-terabytes of data transferred to and from the University of Tennessee's National Institute for Computational Sciences each month. New capabilities available with GridFTP version 5.2.2 include additional transfer log information previously unavailable in prior versions implemented within XSEDE. The transfer log data now available includes identification of source and destination endpoints which unlocks a wealth of information that can be used to detail GridFTP activities across the Internet. This information can be used for a wide variety of reports of interest to individual XSEDE Service Providers and to XSEDE Operations. In this paper, we discuss the new capabilities available for transfer logs in GridFTP 5.2.2, our initial attempt to organize, analyze, and report on this file transfer data for NICS, and its applicability to XSEDE Service Providers. Analysis of this new information can provide insight into effective and efficient utilization of GridFTP resources including identification of potential areas of GridFTP file transfer improvement (e.g., network and server tuning) and potential predictive analysis to improve efficiency.
High isolation and low insertion loss switch IC using GaAs MESFET's (1995)

A novel RF switch IC using GaAs MESFET's has been developed for digital communication systems. The new IC is composed of a three-stage SPST switch and a thin film termination resistor, which realizes a high isolation and a low return loss. In addition, a high power handling capability and a low insertion loss are simultaneously realized with two kinds of pinch-off voltages using the orientation effect of GaAs MESFET's. According to these technologies, the excellent performance is achieved as follows: the isolation of 60 dB, the return loss of 20 dB, the 1 dB power compression of 27 dBm and the insertion loss of 1.6 dB at a frequency of 1.9 GHz with control voltages of 0/-5 V. The new switch IC contributes to a variety of communication system using high-quality digital modulation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Experimental Review of Graphene (2012)

This review examines the properties of graphene from an experimental perspective. The intent is to review the most important experimental results at a level of detail appropriate for new graduate students who are interested in a general overview of the fascinating properties of graphene. While some introductory theoretical concepts are provided, including a discussion of the electronic band structure and phonon dispersion, the main emphasis is on describing relevant experiments and important results as well as some of the novel applications of graphene. In particular, this review covers graphene synthesis and characterization, field-effect behavior, electronic transport properties, magnetotransport, integer and fractional quantum Hall effects, mechanical properties, transistors, optoelectronics, graphene-based sensors, and biosensors. This approach attempts to highlight both the means by which the current understanding of graphene has come about and some tools for future contributions.
Sexually Transmitted Infections and HIV in the Southern United States: An Overview (2006)

From the *Division of STD Prevention, the †Division of HIV/AIDS Prevention-IRS, and the ‡Division of Violence Prevention, National Center for Injury Prevention and Control, Centers for Disease Control and Prevention, Atlanta, Georgia The authors thank Emmet Swint, Patricia Jackson, and Melanie Ross for their outstanding support in the preparation of this article. Correspondence: Sevgi O. Aral, PhD, MSc, MA, Division of STD Prevention, Centers for Disease Control and Prevention (CDC), 1600 Clifton Road, Mailstop E-02, Atlanta, GA 30333. E-mail: [email protected]
Concentration and Separation of Proteins in Microfluidic Channels on the Basis of Transverse IEF (2001)

The use of microfluidic channels formed by two electrodes made of gold or palladium to perform transverse isoelectric focusing (IEF) is presented as a means for continuous concentration and fractionation of proteins. The microchannels were 40 mm long with an electrode gap of 1.27 mm and a depth of 0.354 mm. The properties of pH gradients formed as a result of the electrolysis of water were influenced by variation of parameters such as the initial pH, ionic strength, and flow rate. Transverse IEF in pressure-driven flow is demonstrated using bovine serum albumin in a single ampholyte buffer as well as in multiple-component buffers. Experimental results of protein focusing compare well to predictions of a mathematical model. Optimal conditions for efficient continuous fractionation of a protein mixture are summarized and discussed.
Stimulatory effects of creatine on metabolic activity, differentiation and mineralization of primary osteoblast-like cells in monolayer and micromass cell cultures (2005)

The effects of creatine (Cr) supplementation on primary rat osteoblast-like cells cultured as monolayer and micromass were investigated. Cr was added to the medium at concentrations of either 10 or 20 mM. At various time points, the cell cultures were analyzed morphologically, metabolically and biochemically. The degree of differentiation of primary osteoblast-like cell cultures was higher in micromass cultures compared to monolayer cultures, as judged by alkaline phosphatase (ALP) activity and extent of mineralization. In both culture systems, Cr supplementation showed positive effects, which were dependent on the organizational level of the osteoblast-like cells in such a way that the cells in monolayer culture showed significantly increased metabolic activity, ALP activity and mineralization in the presence of Cr than without the supplement. In micromass cultures, Cr also significantly enhanced ALP activity and mineralization, without affecting metabolic activity. The effect of Cr on ALP activity was more pronounced at higher concentrations of Cr, but 20 mM Cr already showed some adverse effects on cell viability. In conclusion, chemically pure Cr added to low serum cell culture medium has a stimulatory effect on metabolic activity, differentiation and mineralization of osteoblast-like cells indicating that Cr supplementation could also be used as a potential clinical intervention to stimulate cell growth, differentiation and mineralization during bone repair in vivo.