gebhart/patrepeval huggingface dataset:
Rotating-polarizer polarimeter for accurate retardance measurement (1997)

We demonstrate an automated polarimeter based on a rotating polarizer for the measurement of linear retardance independent of laser power and detector gain. The retardance is found when a curve is fitted to a unique normalization of the intensity response of the polarimeter over a range of input polarizer orientations. The performance of this polarimeter is optimal for measurements of quarter-wave retardance and minimal for half-wave retardance. Uncertainties are demonstrated by measurements on six stable double Fresnel rhombs of nominal quarter-wave retardance, yielding expanded uncertainties between 0.031 degrees and 0.067 degrees . The accuracy has also been verified by blind comparisons with interferometric and modified null retardance measurement techniques.
Exchange of oxo ligands in osmium tetroxide with imido ligands in bis(arylimido)bis(tert-butoxo)molybdenum complexes, Mo(NAr)2(O-tert-Bu)2. A facile route to Os(NAr)2O2 and Os(NAr)3O and osmium(IV) complexes of the type Os(NAr)2L2 (NAr = N-2,6-C6H3-iso-Pr2; L = a phosphine) (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExchange of oxo ligands in osmium tetroxide with imido ligands in bis(arylimido)bis(tert-butoxo)molybdenum complexes, Mo(NAr)2(O-tert-Bu)2. A facile route to Os(NAr)2O2 and Os(NAr)3O and osmium(IV) complexes of the type Os(NAr)2L2 (NAr = N-2,6-C6H3-iso-Pr2; L = a phosphine)Jennifer R. Wolf, Guillermo C. Bazan, and Richard R. SchrockCite this: Inorg. Chem. 1993, 32, 19, 4155–4156Publication Date (Print):September 1, 1993Publication History Published online1 May 2002Published inissue 1 September 1993https://pubs.acs.org/doi/10.1021/ic00071a032https://doi.org/10.1021/ic00071a032research-articleACS PublicationsRequest reuse permissionsArticle Views121Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
FIMO: scanning for occurrences of a given motif (2011)

FIMO is part of the MEME Suite software toolkit. A web server and source code are available at http://meme.sdsc.edu.
Extracellular tau is toxic to neuronal cells (2006)

The degeneration of neurons in disorders such as Alzheimer's disease has an immediate consequence, the release of intracellular proteins into the extracellular space. One of these proteins, tau, has proven to be toxic when added to cultured neuronal cells. This toxicity varies according to the degree of protein aggregation. The addition of tau to cultured neuroblastoma cells provoked an increase in the levels of intracellular calcium, which is followed by cell death. We suggest that this phenomenon may be mediated by the interaction of tau with muscarinic receptors, which promotes the liberation of calcium from intracellular stores.
Delivery of protein into cells using polycationic liposomes. (1995)

We describe a procedure for delivery of purified proteins into a variety of tissue culture cells using a new polycationic lipid preparation, LipofectAMINE. Several different proteins, with diverse physical properties, can be delivered into cells by this method. Compared with commercially available monocationic lipids, protein delivery using LipofectAMINE is more efficient. Unlike other methods for protein delivery, the lipofection procedure is simple, inexpensive and effective for both adherent and nonadherent cell types. Proteins introduced into cells using this method are shown to be biochemically and biologically active.
Hierarchical Organization of Prostate Cancer Cells in Xenograft Tumors: The CD44+α2β1+ Cell Population Is Enriched in Tumor-Initiating Cells (2007)

Prostate cancer cells are heterogeneous in their tumorigenicity. For example, the side population cells isolated from LAPC9 xenografts are 100 to 1,000 times more tumorigenic than the corresponding non-side population cells. Highly purified CD44(+) prostate cancer cells from several xenografts are also enriched in prostate cancer stem/progenitor cells. Because the CD44(+) prostate cancer cell population is still heterogeneous, we wonder whether we could further enrich for tumorigenic prostate cancer cells in this population using other markers. Integrin alpha2beta1 has been proposed to mark a population of normal human prostate stem cells. Therefore, we first asked whether the alpha2beta1(+/hi) cells in prostate tumors might also represent prostate cancer stem cells. Highly purified (> or =98%) alpha2beta1(+/hi) cells from three human xenograft tumors, Du145, LAPC4, and LAPC9, show higher clonal and clonogenic potential than the alpha2beta1(-/lo) cells in vitro. However, when injected into the nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse prostate or s.c., the alpha2beta1(+/hi) prostate cancer cells are no more tumorigenic than the alpha2beta1(-/lo) cells. Immunofluorescence studies reveal that CD44 and alpha2beta1 identify an overlapping and inclusive population of prostate cancer cells in that approximately 70% of alpha2beta1(+/hi) cells are CD44(+) and 20% to 30% of CD44(+) cells are distributed in the alpha2beta1(-/lo) cell population. Subsequently, we sorted out CD44(+)alpha2beta1(+/hi), CD44(+)alpha2beta1(-/lo), CD44(-)alpha2beta1(+/hi), and CD44(-)alpha2beta1(-/lo) cells from LAPC9 tumors and carried out tumorigenicity experiments. The results revealed a hierarchy in tumorigenic potential in the order of CD44(+)alpha2beta1(+/hi) approximately CD44(+)alpha2beta1(-/lo) > CD44(-)alpha2beta1(+/hi) >> CD44(-)alpha2beta1(-/lo). These observations together suggest that prostate cancer cells are organized as a hierarchy.
Discordant expression of selectin ligands and sialyl Lewis x–related epitopes on murine myeloid cells (2002)

Murine leukocytes are thought to express alpha2-3-sialylated and alpha1-3-fucosylated selectin ligands such as sialyl Lewis x (sLe(x)), although monoclonal antibodies (mAbs) to sLe(x) or Le(x) reportedly do not bind to murine leukocytes. We observed that P- and E-selectin bound to pronase-sensitive ligands on murine monocytic WEHI-3 cells and murine neutrophils, indicating that the ligands for both selectins are glycoproteins. CSLEX-1, HECA-452, and other widely used mAbs to sLe(x) and Le(x) did not bind to WEHI-3 cells and bound at very low levels to murine neutrophils. Only the anti-sLe(x) mAbs 2H5 and KM93, which also recognize nonfucosylated glycans, bound to WEHI-3 cells. 2H5 and KM93 bound to pronase-resistant structures, indicating that the mAbs did not identify selectin ligands. Treatment of WEHI-3 cells with glycosidases or chlorate demonstrated that sialic acid modifications, alpha1-3-galactosylation, or sulfation did not mask epitopes for mAbs to sLe(x) or Le(x). Compared to human promyelocytic HL-60 cells, WEHI-3 cells and murine neutrophils expressed low alpha1-3-fucosyltransferase activities. Consistent with very low endogenous fucosylation, forced fucosylation of intact WEHI-3 cells or murine neutrophils by exogenous alpha1-3-fucosyltransferase FTVI and GDP-fucose created many new epitopes for anti-sLe(x) mAbs such as HECA-452 and CSLEX-1. Nevertheless, forced fucosylation of intact cells did not significantly augment their ability to bind to fluid-phase P- or E-selectin or to roll on immobilized P- or E-selectin under flow. These data suggest that murine myeloid leukocytes fucosylate only a few specific glycans, which interact preferentially with P- and E-selectin.
Personalizing access control by generalizing access control (2010)

We address the problem of providing data subjects with self-selected controls on access to their personal information. Existing approaches for this are not always sufficient in terms of offering the degrees of control and scope for individualization of access policies that are needed for personal data protection (and usage). We introduce a conceptual framework, a syntax, a semantics, and an axiomatization of a generalized form of access control meta-model, which may be specialized in various ways to enable data subjects to specify flexibly what access controls are to apply on their personal data.
Activating transcription factor-3 (ATF3) functions as a tumor suppressor in colon cancer and is up-regulated upon heat-shock protein 90 (Hsp90) inhibition (2010)

In conclusion, ATF3 down-regulation in colon cancer promotes tumor growth and metastasis. Considering that blocking Hsp90 induces ATF3 expression, Hsp90 inhibition may represent a valid strategy to treat metastatic colon cancer by up-regulating this anti-metastatic transcription factor.
Mechanisms of Spinal Cord Stimulation in Painful Syndromes: Role of Animal Models (2006)

Spinal cord stimulation (SCS) came into clinical practice as a direct spin-off from the "gate control theory." This therapy has been used to treat neuropathic pain of peripheral origin as well as ischemic pain states, vasospastic conditions, and therapy-resistant angina pectoris. The physiological mechanisms of action for SCS are slowly emerging but still are only fragmentarily understood. This chapter will describe the research methods and basic studies used to explore these mechanisms in normal healthy animals, as well as the use of animal models exhibiting different neuropathic, vasculopathic, and other pathological conditions. Brief discussions will address human experimental and clinical studies. The results show that SCS is effective in some pain syndromes otherwise resistant to treatment.
Reexamining the cluster hypothesis (1996)

Article Free Access Share on Reexamining the cluster hypothesis: scatter/gather on retrieval results Authors: Marti A. Hearst Xerox Palo Alto Research Center, 3333 Coyote Hill Rd, Palo Alto, CA Xerox Palo Alto Research Center, 3333 Coyote Hill Rd, Palo Alto, CAView Profile , Jan O. Pedersen Xerox Palo Alto Research Center, 3333 Coyote Hill Rd, Palo Alto, CA Xerox Palo Alto Research Center, 3333 Coyote Hill Rd, Palo Alto, CAView Profile Authors Info & Claims SIGIR '96: Proceedings of the 19th annual international ACM SIGIR conference on Research and development in information retrievalAugust 1996 Pages 76–84https://doi.org/10.1145/243199.243216Online:18 August 1996Publication History 493citation2,028DownloadsMetricsTotal Citations493Total Downloads2,028Last 12 Months64Last 6 weeks9 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
A Randomized Trial of a Low-Carbohydrate Diet for Obesity (2003)

The low-carbohydrate diet produced a greater weight loss (absolute difference, approximately 4 percent) than did the conventional diet for the first six months, but the differences were not significant at one year. The low-carbohydrate diet was associated with a greater improvement in some risk factors for coronary heart disease. Adherence was poor and attrition was high in both groups. Longer and larger studies are required to determine the long-term safety and efficacy of low-carbohydrate, high-protein, high-fat diets.
Immunogenicity of a DNA vaccine candidate for COVID-19 (2020)

The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for the pandemic viral pneumonia disease, COVID-19. At this time, no vaccine is available to control further dissemination of the disease. We have previously engineered a synthetic DNA vaccine targeting the MERS coronavirus Spike (S) protein, the major surface antigen of coronaviruses, which is currently in clinical study. Here we build on this prior experience to generate a synthetic DNA-based vaccine candidate targeting SARS-CoV-2 S protein. The engineered construct, INO-4800, results in robust expression of the S protein in vitro. Following immunization of mice and guinea pigs with INO-4800 we measure antigen-specific T cell responses, functional antibodies which neutralize the SARS-CoV-2 infection and block Spike protein binding to the ACE2 receptor, and biodistribution of SARS-CoV-2 targeting antibodies to the lungs. This preliminary dataset identifies INO-4800 as a potential COVID-19 vaccine candidate, supporting further translational study.
A 7 µm mini-beam improves diffraction data from small or imperfect crystals of macromolecules (2008)

A simple apparatus for achieving beam sizes in the range 5-10 μm on a synchrotron beamline was implemented in combination with a small 125 x 25 μm focus. The resulting beam had sufficient flux for crystallographic data collection from samples smaller than 10 x 10 x 10 μm. Sample data were collected representing three different scenarios: (i) a complete 2.0 data set from a single strongly diffracting microcrystal, (ii) a complete and redundant 1.94 A data set obtained by merging data from six microcrystals and (iii) a complete 2.24 A data set from a needle-shaped crystal with less than 12 x 10 μm cross-section and average diffracting power. The resulting data were of high quality, leading to well refined structures with good electron-density maps. The signal-to-noise ratios for data collected from small crystals with the mini-beam were significantly higher than for equivalent data collected from the same crystal with a 125 x 25 μm beam. Relative to this large beam, use of the mini-beam also resulted in lower refined crystal mosaicities. The mini-beam proved to be advantageous for inhomogeneous large crystals, where better ordered regions could be selected by the smaller beam.
Creating dynamic SERS hotspots on the surface of pH-responsive microgels for direct detection of crystal violet in solution (2017)

Creation of dynamic SERE hotspots controlled by pH values on the surface of gold nanoparticles-loaded pH-responsive P2VP microgels, using 4-mercaptobenzoic acid (PMBA) as a probe molecule.
Stimulation of Neonatal and Adult Brain Neurogenesis by Subcutaneous Injection of Basic Fibroblast Growth Factor (1999)

Mounting evidence indicates that extracellular factors exert proliferative effects on neurogenetic precursors in vivo. Recently we found that systemic levels of basic fibroblast growth factor (bFGF) regulate neurogenesis in the brain of newborn rats, with factors apparently crossing the blood-brain barrier (BBB) to stimulate mitosis. To determine whether peripheral bFGF affects proliferation during adulthood, we focused on regions in which neurogenesis persists into maturity, the hippocampus and the forebrain subventricular zone (SVZ). In postnatal day 1 (P1) rats, 8 hr after subcutaneous injection (5 ng/gm body weight), bFGF increased [(3)H]thymidine incorporation 70% in hippocampal and SVZ homogenates and elicited twofold increases in mitotic nuclei in the dentate gyrus and the dorsolateral SVZ, detected by bromodeoxyuridine immunohistochemistry. Because approximately 25% of proliferating hippocampal cells stimulated in vivo expressed neuronal traits in culture, bFGF-induced mitosis may reflect increased neurogenesis. bFGF effects were not restricted to the perinatal period; hippocampal DNA synthesis was stimulated by peripheral factor in older animals (P7-P21), indicating the persistence of bFGF-responsive cells and activity of peripheral bFGF into late development. To begin defining underlying mechanisms, pharmacokinetic studies were performed in P28 rats; bFGF transferred from plasma to CSF rapidly, levels rising in both compartments in parallel, indicating that peripheral factor crosses the BBB during maturity. Consequently, we tested bFGF in adults; peripheral bFGF increased the number of mitotic nuclei threefold in the SVZ and olfactory tract, regions exhibiting persistent neurogenesis. Our observations suggest that bFGF regulates ongoing neurogenesis via a unique, endocrine-like pathway, potentially coordinating neuron number and body growth, and potentially providing new approaches for treating damaged brain during development and adulthood.
[Dissection technique--is ultrasound the best method?]. (2001)

Preventive hemostasis is extremely important in endoscopic surgery. Ultrasonic dissectors are used very often. We tested the occlusion safety of bipolar forceps and ultrasonic dissector for porcine vessels. Thermographic videos showed maximum temperature up to 200 degrees C when using one ultrasonic dissector. The lateral damage zone in vivo and in vitro measured between 2 and 6 mm.
Subclass Restriction of Murine Anti-Carbohydrate Antibodies (1978)

Examination of the subclass distribution of murine antibodies directed against groups A and C streptococcal carbohydrate, alpha-(1 leads to 3) dextran and phosphocholine yields the surprising observation that these carbohydrate antigens stimulate IgG responses largely restricted to the rare IgG3 subclass. This subclass restriction is particularly impressive in light of the low circulating levels of IgG3 in nonimmune mouse serum and the failure of a variety of other antigens including proteins and aromatic haptens to stimulate IgG3 antibody production. Attempts to alter the subclass restriction of antibodies with carbohydrate specificity by immunization with carbohydrate-coupled protein have been unsuccessful and indicate that immunoregulation of subclass expression probably occurs at the level of the antibody forming (B) cell. It is therefore conceivable that VH regions of murine immunoglobulins may be restricted to particular IgG subclasses. A similar type of subclass restriction has been reported in human and rat anti-carbohydrate antibodies. This recruitment of a minor immunoglobulin isotype by carbohydrate antigens in several species further supports the concept of immunoregulation at the level of subclass, and suggests that these and other mammals may share a structurally similar isotype with perhaps a common evolutionary origin.
Heparin-binding EGF-like growth factor stimulation of smooth muscle cell migration: dependence on interactions with cell surface heparan sulfate (1993)

Heparin-binding EGF-like growth factor (HB-EGF), but not EGF, binds to cell surface heparan sulfate proteoglycan (HSPG). This was demonstrated in (a) the binding of 125I-HB-EGF to mutant CHO cells deficient in HS production was diminished by 70% compared to wild-type CHO cells, (b) the binding of 125I-HB-EGF to CHO cells and bovine aortic smooth muscle cells (BASMC) was diminished 80% by heparitinase or chlorate treatment, and (c) 125I-EGF did not bind to CHO cells and its binding to BASMC was not diminished at all by heparitinase and only slightly by chlorate treatment. Accordingly, the role of HB-EGF interactions with HSPG in modulating bioactivity was examined. Heparitinase or chlorate treatment of BASMC diminished the ability of HB-EGF to stimulate BASMC migration by 60-80%. A similar inhibition of migration occurred when BASMC were treated with a synthetic peptide (P21) corresponding to the sequence of the putative heparin-binding domain of HB-EGF. As a control for BASMC viability, and for specificity, it was found that heparitinase and P21 did not inhibit at all and chlorate inhibited only slightly the stimulation of BASMC migration by PDGF AB. Since heparitinase, chlorate, and P21 treatment also diminished by 70-80% the cross-linking of 125I-HB-EGF to the EGF receptor, it was concluded that the interaction of HB-EGF, via its heparin-binding domain, with cell surface HSPG was essential for its optimal binding to the EGF receptor on BASMC and hence for its optimal ability to stimulate migration.
ATRIAL NATRIURETIC FACTOR AND RELATED PEPTIDE HORMONES (1991)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Structure and expression of the fliA operon of Salmonella typhimurium (1999)

The fliA gene encodes the flagellum-specific sigma factor sigma28 In Salmonella typhimurium. The transcription in vivo and in vitro of this gene was analysed and it was found that there are two promoters for the expression of this gene. One is a class 2 promoter which is recognized by sigma70-RNA polymerase in the presence of the FlhD and FlhC activator proteins. The other is a class 3 promoter which is recognized by sigma28-RNA polymerase. Therefore, the fliA operon is under dual positive control from FlhD/FlhC and from FliA itself. The nucleotide sequence downstream of the fliA gene was determined. The sequence contains two ORFs following the fliA gene. On the basis of their sequence homology, it is concluded that these two correspond to the fliZ and fliY genes of Escherichia coil. Northern blot analysis revealed that the fliZ gene is transcribed from the fliA promoters, whereas the fliY gene is transcribed from both the fliA promoters and its own FlhD/FlhC-independent promoter. A fliZ-disruption mutant was constructed by inserting a kanamycin-resistance gene cassette into the fliZ gene on the chromosome. The mutant showed poor motility, and introduction of a fliZ+ plasmid into this mutant restored the wildtype level of motility. These results suggest that the fliZ gene may be required for expression of maximal motility.
PLANT SUCCESSION ON SICILIAN TERRACES (2007)

189 phytosociological relev&amp;eacute;s have been made in five areas of Sicily, three on volcanic substrates and two on limestones, to study plant community succession trends within abandoned terraced vineyards and cereal fields. Disturbance status and abandonment age was recorded for each sample plot. DCA of these relev&amp;eacute;s suggests that four are the most important factors driving succession, i.e. geological substrate, bioclimate, age of abandonment and disturbance. In most cases - if there are some dispersal centres near the old fields - undisturbed and less disturbed terraces evolve quite rapidly towards the local potential &amp;ldquo;climax&amp;rdquo; community. Different trends have been recorded on the areas which underwent and still undergo frequent and severe disturbance: succession is biased or even blocked in a steady state, so that grassland, garrigue, mantle or herb-dominated communities prevail. The species composition of the plant communities subject to disturbance seems to be strongly connected with the time gap between abandonment and first disturbance occurrence. The &amp;ldquo;mixed&amp;rdquo; ecological-structural-phytosociological approach here adopted to describe plant communities was quite effective to interpret abandoned terraces communities and their dynamism.