gebhart/patrepeval huggingface dataset:
Quantitative proteomics analysis of BMS ‐214662 effects on CD 34 positive cells from chronic myeloid leukaemia patients (2012)

Chronic myeloid leukaemia (CML) arises in a haemopoietic stem cell and is driven by the Bcr-Abl oncoprotein. Abl kinase inhibitors (protein tyrosine kinase inhibitors) represent standard treatment for CML and induce remission in the majority of patients with early disease, however these drugs do not target leukaemic stem cells (LSCs) effectively, thus preventing cure. Previously, we identified the farnesyl transferase inhibitor BMS-214662 as a selective inducer of apoptosis in LSCs of CML patients relative to normal controls; however, the mechanism underlying LSC-specific apoptosis remains unclear. To identify pathways involved in the favourable effects of BMS-214662 in CML, we employed a proteomic approach (based on iTRAQ) to analyse changes in protein expression in response to drug treatment in the nuclear and cytoplasmic fractions of CD34(+) CML cells. The study identified 88 proteins as altered after drug treatment, which included proteins known to be involved in nucleic acid metabolism, oncogenesis, developmental processes and intracellular protein trafficking. We found that expression of Ebp1, a negative regulator of proliferation, was upregulated in the nucleus of BMS-214662-treated cells. Furthermore, proteins showing altered levels in the cytosol, such as histones, were predominantly derived from the nucleus and BMS-214662 affected expression levels of nuclear pore complex proteins. Validation of key facets of these observations suggests that drug-induced alterations in protein localisation, potentially via loss of nuclear membrane integrity, contributes to the LSC specificity of BMS-214662, possibly via Ran proteins as targets.
Antiparallel β-sheet: a signature structure of the oligomeric amyloid β-peptide (2009)

AD (Alzheimer's disease) is linked to Abeta (amyloid beta-peptide) misfolding. Studies demonstrate that the level of soluble Abeta oligomeric forms correlates better with the progression of the disease than the level of fibrillar forms. Conformation-dependent antibodies have been developed to detect either Abeta oligomers or fibrils, suggesting that structural differences between these forms of Abeta exist. Using conditions which yield well-defined Abeta-(1-42) oligomers or fibrils, we studied the secondary structure of these species by ATR (attenuated total reflection)-FTIR (Fourier-transform infrared) spectroscopy. Whereas fibrillar Abeta was organized in a parallel beta-sheet conformation, oligomeric Abeta displayed distinct spectral features, which were attributed to an antiparallel beta-sheet structure. We also noted striking similarities between Abeta oligomers spectra and those of bacterial outer membrane porins. We discuss our results in terms of a possible organization of the antiparallel beta-sheets in Abeta oligomers, which may be related to reported effects of these highly toxic species in the amyloid pathogenesis associated with AD.
Honey for Decubitus Ulcers (1973)

To the Editor.— With reference to Dr. James Barnes' work using granulated sugar in the healing of ulcers (223-122, 1973), I should like to mention a somewhat similar traditional remedy, the use of honey, for such problems. I have been using pure natural honey for the past few months in the accident and emergency departments where I work, and I have found that, applied every two or three days under a dry dressing, it promotes the healing of ulcers and burns better than any other local applications I've used before. It can also readily be applied to any other surface wounds, including cuts and abrasions, and I can recommend it to all doctors as a very inexpensive and valuable cleansing and healing agent. It even has a delicious taste!
Amyloid-? proteins activate Ca2+-permeable channels through calcium-sensing receptors (1997)

The amyloid-beta peptides (A beta) are produced in excess in Alzheimer's disease (AD) and may contribute to neuronal dysfunction and degeneration. This study provides strong evidence for a novel cellular target for the actions of A beta, the phospholipase C-coupled, extracellular Ca(2+)-sensing receptor (CaR). We demonstrate that A beta(s) produce a CaR-mediated activation of a Ca(2+)-permeable, nonselective cation channel (NCC), probably via elevation in cytosolic Ca2+ (Cai), in cultured hippocampal pyramidal neurons from normal rats and from wild type mice but not those from mice with targeted disruption of the CaR gene (CaR -/-). A beta(s) also activate NCC in CaR-transfected but not in nontransfected human embryonic kidney (HEK293) cells. Thus aggregates of A beta deposited on hippocampal neurons in AD could appropriately activate the CaR, stimulating Ca(2+)-permeable channels and causing sustained elevation of Cai with resultant neuronal dysfunction.
Irradiation of Linear Polyethylene. Partitioning between Sol and Gel (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIrradiation of Linear Polyethylene. Partitioning between Sol and GelA. Rijke and L. MandelkernCite this: Macromolecules 1971, 4, 5, 594–599Publication Date (Print):September 1, 1971Publication History Published online1 May 2002Published inissue 1 September 1971https://pubs.acs.org/doi/10.1021/ma60023a016https://doi.org/10.1021/ma60023a016research-articleACS PublicationsRequest reuse permissionsArticle Views85Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Integrating microfabricated fluidic systems and NMR spectroscopy (2000)

The philosophy of miniature total analysis systems (mu-TAS) hinges on the integration of multiple chemical processing steps and the means of analyzing their results on the same miniaturized system. We have constructed chip-based capillary electrophoresis (CE) devices equipped with an integrated planar radio-frequency detector coil used for nuclear magnetic resonance spectroscopy (NMR). Separations were accomplished in the devices, but satisfactory NMR spectra could only be obtained from samples of high concentration. The relative sensitivity is explained and the scaling law dichotomy of CE and NMR explored.
Extreme Learning Machine based fast object recognition (2012)

Extreme Learning Machine (ELM) as a type of generalized single-hidden layer feed-forward networks (SLFNs) has demonstrated its good generalization performance with extreme fast learning speed in many benchmark and real applications. This paper further studies the performance of ELM and its variants in object recognition using two different feature extraction methods. The first method extracts texture features, intensity features from Histogram and features from two types of color space: HSV &amp; RGB. The second method extracts shape features based on Radon transform. The classification performances of ELM and its variants are compared with the performance of Support Vector Machines (SVMs). As verified by simulation results, ELM achieves better testing accuracy with much less training time on majority cases than SVM for both feature extraction methods. Besides, the parameter tuning process for ELM is much easier than SVM as well.
On Biomineralization (1989)

Focusing on the basic principles of mineral formation by organisms, this comprehensive volume explores questions that relate to a wide variety of fields, from biology and biochemistry, to paleontology, geology, and medical research. Preserved fossils are used to date geological deposits and archaeological artifacts. Materials scientists investigate mineralized tissues to determine the design principles used by organisms to form strong materials. Many medical problems are also associated with normal and pathological mineralization. Lowenstam, the pioneer researcher in biomineralization, and Weiner discuss the basic principles of mineral formation by organisms and compare various mineralization processes. Reference tables listing all known cases in which organisms form minerals are included.
Crosstab, OLAP, and APL (1998)

OLAP is the process of creating and Online-managing multidimensional data, but what is the difference between CROSSTAB and OLAP? It is difficult to answer this question in a few words. Let us not waste time trying to find precise definitions, let us rather agree on the fact that compared with CROSSTAB OLAP is the technicaUy more advanced method.
Intracerebral microdialysis: 30 years as a tool for the neuroscientist (2003)

1. Microdialysis is an established technique for studying physiological, pharmacological and pathological changes of a wide range of low molecular weight substances in the brain extracellular fluid. Many studies have proven its sensitivity in sampling the extracellular space in discrete brain locations, such as the striatum, and monitoring the action of exogenous substances. 2. The two main areas of application of microdialysis are the recovery of endogenous substances, primarily the neurotransmitters, and the infusion of drugs through the microdialysis cannula (retrodialysis). 3. Microdialysis in awake animals is the tool of choice for studying the relationship between changes in behaviour and neurotransmitters in certain brain areas. In addition, the concomitant recording of the electroencephalogram at the site of microdialysis has been shown to be extremely useful in determining the role of certain neurotransmitters in paroxysmal activity. 4. Clinical applications of microdialysis have included monitoring of ischaemic injury, subarachnoid haemorrhage, trauma and epilepsy. With the recent availability of standardized equipment, the use of microdialysis in the neurological clinic is likely to become more common.
Contrasting effects of interleukin 4 (IL-4) on the in vitro proliferation of human B cells is due to the presence of B cell subsets in different activation states. (1990)

Human tonsillar B lymphocytes proliferate in vitro after activation with immobilized anti-IgM antibodies in combination with interleukin 2 (IL-2) or interleukin 4 (IL-4). In addition to its proliferative effects, IL-4 is also able to inhibit B cell proliferation. An explanation for these different actions of the same protein is not as yet available. The susceptibility to the inhibitory action of IL-4 emerges at late time points after in vitro activation of the B cells, whereas IL-4 shows its growth promoting action during early stages of culture, indicating that activated IL-2 responsive B cells are the targets for IL-4 dependent inhibition. Freshly isolated tonsillar B lymphocytes also appear to be susceptible to IL-4 dependent suppression. Isotonic Percoll gradient centrifugation, that has been used by several groups as a standard procedure to isolate high density, resting used by several groups as a standard procedure to isolate high density, resting cells from tonsil B lymphocytes, does not result in separation of B cells with different in vitro proliferative response to IL-2 and IL-4. However, partial separation of cells with "resting" and "activated" phenotypes can be achieved by centrifugation in slightly hypotonic Percoll gradients. High-density fractions contain cells with a "resting" phenotype which after in vitro activation proliferate with IL-4 but not with IL-2. "Activated" B cells accumulate in the low-density fractions as concluded from the increased expression of transferrin receptors and HLA-DR molecules on these cells. However, these cells no longer proliferate in vitro with immobilized anti-IgM antibodies, possibly due to damage caused by the hypotonic treatment during centrifugation. Our data indicate that resting B cells when activated in vitro with immobilized anti-IgM antibodies proliferate primarily with IL-4 but not with IL-2. In contrast, about 15 hours after activation the cells become responsive to IL-2. At that stage, IL-4 becomes an inhibitory factor.
Dual-Band Circularly Polarized Stacked Annular-Ring Patch Antenna for GPS Application (2011)

A dual-band circularly polarized stacked annular-ring patch antenna is presented in this letter. This antenna operates at both the GPS L1 frequency of 1575 MHz and L2 frequency of 1227 MHz, whose frequency ratio is about 1.28. The proposed antenna is formed by two concentric annular-ring patches that are placed on opposite sides of a substrate. Wide axial-ratio bandwidths (larger than 2%), determined by 3-dB axial ratio, are achieved at both bands. The measured gains at 1227 and 1575 MHz are about 6 and 7 dBi, respectively, with the loss of substrate taken into consideration. Both simulated and measured results are presented. The method of varying frequency ratio is also discussed.
Claiming Rank: The Display of Wealth and Status by Eighteenth-Century Lunenburg, Nova Scotia, Merchants (1992)

This paper is concerned with the history, architecture, and proxemic patterns of the Knaut-Rhuland house in Lunenburg, Nova Scotia. Built in 1793 by Benjamin Knaut, the son of Phillip Augustus Knaut, it boasts a Georgian interior with central hall and seven fireplaces, servants' quarters in the attic, and double hearths and bakeovens in two basement kitchens. As a merchant house it is in keeping with the size and style of house occupied by the merchant class in Lunenburg (and other parts of Nova Scotia) as a mark of their wealth and social status, and as such is examined as part of an interlocking oligarchy common to other merchant families which helped shape the social, cultural and material context of the community.
 Resume
 
L'article traite de l'histoire, de l'architecture et de la relation de proximite des pieces de la maison Knaut-Rhuland a Lunenburg (Nouvelle-Ecosse). Construite en 1793 par Benjamin Knaut, fils de Phillip Augustus Knaut, cette maison arbore un interieur de style georgien avec hall central, sept foyers, des chambres de domestiques sous les combles et des foyers et fours a pain doubles dans les deux cuisines situees au sous-sol. Cette maison de marchand a les dimensions et le style d'architecture courants des maisons des marchands de Lunenburg (et d'ailleurs en Nouvelle-Ecosse) et qui indiquaient la richesse et le statut social auxquels leur classe etait parvenue. A ce titre, elle est etudiee en tant qu'element d'une oligarchie de familles marchandes liees les unes aux autres par des liens etroits, qui ont contribue a la formation du tissu social, de la vie culturelle et de la culture materielle de leur communaute.
Isolating Fetal Cells From Maternal Blood (1993)

Isolating and analyzing fetal cells from maternal blood is clearly possible. Several key biologic questions remain--the optimal cells for isolation, frequency of cells in maternal blood, timing during gestation for maternal blood sampling, and the likelihood of persistence of fetal cells after delivery. Clinical evaluations planned by the National Institute of Child Health and Human Development will determine the sensitivity and specificity of this method and its precise role in prenatal cytogenetic diagnosis.
Linear time erasure codes with nearly optimal recovery (2002)

An (n,c,l,r) erasure code consists of an encoding algorithm and a decoding algorithm with the following properties. The encoding algorithm produces a set of l-bit packets of total length cn from an n-bit message. The decoding algorithm is able to recover the message from any set of packets whose total length is r, i.e., from any set of r/l packets. We describe erasure codes where both the encoding and decoding algorithms run in linear time and where r is only slightly larger than n.
Compaction with incremental over-constraint resolution (1988)

The occurrence and resolution of over-constraints in one-dimensional layout compaction will be discussed. A new algorithm is given which solves the longest path problem in the constraint graph and resolves all positive cycles either by constraint relaxation or by jog generation. The presented algorithm eliminates the positive cycles one at a time and reuses the intermediate results, which had been obtained up to the point when the cycle was detected. Thus, the amount of additional effort required for the over-constraint resolution is very low. Some typical over-constrained situations and their solutions are shown. The algorithm has been implemented in a mask compaction program that is currently in use for process migration and design rule error correction at SIEMENS. CPU-times gained during the process migration of a microcontroller layout are given.
A 650-MHz, IA-32 microprocessor with enhanced data streaming for graphics and video (1999)

This paper describes a new IA-32 architecture microprocessor that implements 70 additional instructions to further accelerate the performance of data-streaming applications such as three-dimensional graphics and video encode/decode. This processor is an enhancement over the previous implementation of this family through the addition of these new instructions along with circuit improvements in several key areas for higher clock frequency. The 10.17/spl times/12.10 mm/sup 2/ die contains 9.5 million transistors and is fabricated in a CMOS five-layer-metal 0.25-/spl mu/m process with a six-layer organic land grid array package using C4 interconnect technology. It has an operating range of 1.4-2.2 V and is currently running up to 650 MHz.
Small non-coding RNAs and genomic imprinting (2006)

Experimental and computer-assisted approaches have led to the identification of hundreds of imprinted small RNA genes, mainly clustered in two chromosomal domains (human 15q11-->q13 and 14q32 loci). The genes are only detected in placental mammals and belong to the C/D RNA and microRNA gene families. These are small non-coding RNAs involved in RNA-guided post-transcriptional RNA modifications and RNA-mediated gene silencing, respectively. Here, we discuss their potential functions and report the identification of novel small RNA genes lying within (or nearby) known imprinted chromosomal domains.
Regulation of the expression of components of the exocytotic machinery of insulin-secreting cells by microRNAs (2008)

Fine-tuning of insulin secretion from pancreatic beta-cells participates in blood glucose homeostasis. Defects in this process can lead to chronic hyperglycemia and diabetes mellitus. Several proteins controlling insulin exocytosis have been identified, but the mechanisms regulating their expression remain poorly understood. Here, we show that two non-coding microRNAs, miR124a and miR96, modulate the expression of proteins involved in insulin exocytosis and affect secretion of the beta-cell line MIN6B1. miR124a increases the levels of SNAP25, Rab3A and synapsin-1A and decreases those of Rab27A and Noc2. Inhibition of Rab27A expression is mediated by direct binding to the 3'-untranslated region of Rab27A mRNA. The effect on the other genes is indirect and linked to changes in mRNA levels. Over-expression of miR124a leads to exaggerated hormone release under basal conditions and a reduction in glucose-induced secretion. miR96 increases mRNA and protein levels of granuphilin, a negative modulator of insulin exocytosis, and decreases the expression of Noc2, resulting in lower capacity of MIN6B1 cells to respond to secretagogues. Our data identify miR124a and miR96 as novel regulators of the expression of proteins playing a critical role in insulin exocytosis and in the release of other hormones and neurotransmitters.
Perpendicular magnetic anisotropy of Ir/CoFeB/MgO trilayer system tuned by electric fields (2015)

The perpendicular magnetic anisotropy of an Ir/CoFeB/MgO trilayer was investigated after annealing at temperatures ranging from 200 to 350 °C. In the trilayer system annealed at 300 °C, we measured an interface anisotropy energy of 1.9 mJ/m2. Further annealing led to mixing of the buffer and ferromagnet, degrading the properties of the latter. In addition, we show the dependence of the magnetic anisotropy on the bias voltage. The presented system is important for the development of perpendicular magnetic tunnel junctions for storage applications.
Angiotensin II Type 1 Receptor Antagonist, TCV-116, Prevents Neointima Formation in Injured Arteries in the Dog (1999)

We investigated the effect of an angiotensin (Ang) II antagonist, (+/-)-1-(cyclohexyloxycarbonyloxy)-ethyl 2-ethoxy- 1- [[2'-(1H-tetrazol-5-yl)biphenyl-4-yl]methyl]- 1H-benzimidazole-7-carboxylate (TCV-116), on neointima formation in dog artery injured by a balloon catheter. Dogs were orally treated with 10 mg/kg TCV-116 or placebo twice a day for 5 weeks. After treatment with these drugs for 1 week, the right carotid artery was injured by a balloon catheter. The left carotid artery was regarded as the control. In the group treated with placebo, neointima formation in the injured arteries was observed. The activities of angiotensin converting enzyme (ACE) and chymase in the injured carotid arteries were increased 2.56- and 3.26-fold compared with those in the non-injured arteries, respectively. The neointimal area in dogs treated with placebo and TCV-116 were 0.51 +/-0.07 and 0.21 +/-0.07 mm2, respectively, and this difference was significant. In conclusion, an Ang II antagonist, TCV-116, prevented neointima formation by blocking the action of Ang II generated by both ACE and chymase in the injured arteries.
The Vault Exterior Shell Is a Dynamic Structure that Allows Incorporation of Vault-Associated Proteins into Its Interior (2006)

Vaults are 13 million Da ribonucleoprotein particles with a highly conserved structure. Expression and assembly by multimerization of an estimated 96 copies of a single protein, termed the major vault protein (MVP), is sufficient to form the minimal structure and entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, VPARP and TEP1, and a small untranslated vault RNA are also associated with vaults. We used the Sf9 insect cell expression system to form MVP-only recombinant vaults and performed a series of protein-mixing experiments to test whether this particle shell is able to exclude exogenous proteins from interacting with the vault interior. Surprisingly, we found that VPARP and TEP1 are able to incorporate into vaults even after the formation of the MVP vault particle shell is complete. Electrospray molecular mobility analysis and spectroscopic studies of vault-interacting proteins were used to confirm this result. Our results demonstrate that the protein shell of the recombinant vault particle is a dynamic structure and suggest a possible mechanism for in vivo assembly of vault-interacting proteins into preformed vaults. Finally, this study suggests that the vault interior may functionally interact with the cellular milieu.