gebhart/patrepeval huggingface dataset:
Structural Requirements for the Assembly of Norwalk Virus-Like Particles (2002)

Norwalk virus (NV) is the prototype strain of a group of human caliciviruses responsible for epidemic outbreaks of acute gastroenteritis. While these viruses do not grow in tissue culture cells or animal models, expression of the capsid protein in insect cells results in the self-assembly of recombinant NV virus-like particles (rNV VLPs) that are morphologically and antigenically similar to native NV. The X-ray structure of the rNV VLPs has revealed that the capsid protein folds into two principal domains: a shell (S) domain and a protruding (P) domain (B. V. V. Prasad, M. E. Hardy, T. Dokland, J. Bella, M. G. Rossmann, and M. K. Estes, Science 286:287-290, 1999). To investigate the structural requirements for the assembly of rNV VLPs, we performed mutational analyses of the capsid protein. We examined the ability of 10 deletion mutants of the capsid protein to assemble into VLPs in insect cell cultures. Deletion of the N-terminal 20 residues, suggested by the X-ray structure to be involved in a switching mechanism during assembly, did not affect the ability of the mutant capsid protein to self-assemble into 38-nm VLPs with a T=3 icosahedral symmetry. Further deletions in the N-terminal region affected particle assembly. Deletions in the C-terminal regions of the P domain, involved in the interactions between the P and S domains, did not block the assembly process, but they affected the size and stability of the particles. Mutants carrying three internal deletion mutations in the P domain, involved in maintaining dimeric interactions, produced significantly larger 45-nm particles, albeit in low yields. The complete removal of the protruding domain resulted in the formation of smooth particles with a diameter that is slightly smaller than the 30-nm diameter expected from the rNV structure. These studies indicate that the shell domain of the NV capsid protein contains everything required to initiate the assembly of the capsid, whereas the entire protruding domain contributes to the increased stability of the capsid by adding intermolecular contacts between the dimeric subunits and may control the size of the capsid.
Effects of Once-Daily and Twice-Daily Dosing on Adherence With Prescribed Glipizide Oral Therapy for Type 2 Diabetes (2002)

Initiation of OD pharmacotherapy results in better adherence and persistence compared with a BID regimen, despite a greater daily pill burden in the OD cohort. These data suggest that dosing frequency exerts a greater impact on patient adherence and persistence than number of tablets per dose.
Design of Triazole-Stapled BCL9 α-Helical Peptides to Target the β-Catenin/B-Cell CLL/lymphoma 9 (BCL9) Protein–Protein Interaction (2011)

The interaction between β-catenin and B-cell CLL/lymphoma 9 (BCL9), critical for the transcriptional activity of β-catenin, is mediated by a helical segment from BCL9 and a large binding groove in β-catenin. Design of potent, metabolically stable BCL9 peptides represents an attractive approach to inhibit the activity of β-catenin. In this study, we report the use of the Huisgen 1,3-dipolar cycloaddition reaction to generate triazole-stapled BCL9 α-helical peptides. The high efficiency and mild conditions of this "click" reaction combined with the ease of synthesis of the necessary unnatural amino acids allows for facile synthesis of triazole-stapled peptides. We have performed extensive optimization of this approach and identified the optimal combinations of azido and alkynyl linkers necessary for stapling BCL9 helices. The unsymmetrical nature of the triazole staple also allowed the synthesis of double-stapled BCL9 peptides, which show a marked increase in helical character and an improvement in binding affinity and metabolic stability relative to wild-type and linear BCL9 peptides. This study lays the foundation for further optimization of these triazole-stapled BCL9 peptides as potent, metabolically stable, and cell-permeable inhibitors to target the β-catenin and BCL9 interaction.
The p53 gene and its role in human brain tumors (1995)

Mutation of the p53 gene is among the most common lesions in a variety of human tumors, including those of the central nervous system. In most instances, mutation of one p53 allele is followed by loss of the remaining wild-type allele, resulting in cells with a complete absence of functional wild-type p53 protein. However, in some situations, such as at initiation of spontaneously arising gliomas or as the germline configuration of patients with the Li-Fraumeni syndrome, cells clearly carry both wild-type and mutant p53 alleles. These observations lead to the hypothesis that p53 mutations can give rise to loss of tumor suppressor functions as well as to gain of oncogenic transformation capabilities. In this review, we define the types of mutations that occur in the p53 gene in various glial tumors, contrast that with the spectra described in other human tumor types, and discuss the biochemistry and physiology of the p53 protein and its ability to regulate and be regulated by other gene products. We use this information to propose roles for p53 in the initiation and progression of human gliomas.
Elevated Temperature Structural Testing of Advanced Missiles (1982)

Covers advancements in spacecraft and tactical and strategic missile systems, including subsystem design and application, mission design and analysis, materials and structures, developments in space sciences, space processing and manufacturing, space operations, and applications of space technologies to other fields.
Assay of circulating immune complexes by immunonephelometry markers of immunonephropathy among young Nigerians. (1990)

Earlier workers had noted that chronic renal insufficiency accounts for 1.6% of the total medical admissions in a tertiary health institution of Nigeria. Most of cases occur among Nigerians under 40 years of age, which stimulated our interest in the present study. Using laser immunonephelometry, we quantitated the circulating immune complexes (CIC) and correlated the results with those of the routine renal biochemistry. We found a high load of CIC among all the subjects studied. These complexes were unrestricted to any particular immunoglobulin class. Higher correlation was obtained between the serum urea and the sum total of the immunoglobulin classes, suggesting that polyclonal antigens may be involved in the immunonephropathy. We observed a peculiar curvilinear trend, when a consistent rise in the serum urea was correlated with an increase, then a decline in all the Ig classes. This threshold may mark the development of immune paralysis and subsequent renal shutdown.
A cop on the beat: Collecting and appraising intrusion evidence (1999)

The article discusses the importance of intrusion detection system in an environment of increased dependency on computer system networks. The field of intrusion detection began close to 20 years ago. The majority of early systems were designed to detect attacks upon a single host. More recent systems consider the role of networks and look for evidence of intrusions by passively monitoring LAN traffic. Yet another set of intrusion detection systems is designed to collect and aggregate evidence from multiple sources in order to detect coordinated or multistage attacks on a network. Such evidence fusion is an important capability since, in general, a network is vulnerable to attacks distributed across its hosts. Network monitoring tools can play multiple intrusion detection roles. The first, and most obvious, is for the detection of known patterns of intrusive behavior. Alternatively, network monitors can be used to assist in tracking intruders after an attack has been detected, using a statistical profile of the users' activity as a thumbprint to determine the connections used during an attack.
CD137 and CD137 ligand constitutively coexpressed on human T and B leukemia cells signal proliferation and survival (2003)

CD137, a member of the tumor necrosis factor receptor family, provides expansion and survival signal to T cells. Its ligand, CD137L, in addition to its ability to costimulate T cells, signals back into antigen presenting cells promoting their activation and differentiation. Recently, CD137 has been proposed as a therapeutic target to improve and sustain anticancer immune response. Several activated T leukemia and B lymphoma cell lines expressed CD137 or CD137L, respectively, and soluble CD137L has been found in sera of leukemia patients. However, the functionality and role of these costimulatory molecules in hematologic malignancies are until now unknown. Interestingly, we observed constitutive CD137 and CD137L coexpression on both human T and B leukemia cell lines. The constitutive CD137 expression on unstimulated T or B leukemia cells presents some differences compared to CD137 expressed on PMA/ionomycin-activated T leukemia cells. Surprisingly, in spite of the low expression level, both tumor CD137 and CD137L molecules signaled in T and B leukemia cells inducing proliferation and prolonging survival. In addition, CD137/CD137L system ligation opposed the anticancer drug cytotoxic effects, reducing the apoptotic DNA fragmentation and stimulating proliferation of doxorubicin-escaped leukemia cells. Although the role of leukemia CD137/CD137L system in vivo is unknown, these data suggest that these costimulatory molecules might confer an advantage to hematologic tumors promoting survival, sustaining cellular growth and contributing to drug resistance.
Polar Hinges as Functionalized Conformational Constraints in (Bi)cyclic Peptides (2016)

Two polar hinges for cyclization of peptides have been developed, leading to bicyclic peptides and cyclized peptides with improved solubility and biological activity. Increasingly, we note that a good aqueous solubility of peptides is an absolute prerequisite, not only to allow handling and purification of our target peptides but also being crucial for biological activity characteristics. Compared to earlier hinges, the 1,1',1"-(1,3,5-triazinane-1,3,5-triyl)tris(2-bromoethanone) (TATB) and 2,4,6-tris(bromomethyl)-s-triazine (TBMT), each containing three nitrogen atoms are structurally similar but chemically very different. Both were accessible in a one-step fashion from bromoacetonitrile. TATB and TBMT are very suitable for the preparation of more soluble bicyclic peptides. Azide-modified TATB and TBMT derivatives provide hinges for the preparation of cyclized peptides for incorporation on scaffolds to afford protein mimics.
Biosynthesis of Polyunsaturated Fatty Acids by Polyketide Synthases (2002)

Psychrophilic marine bacteria can produce polyunsaturated fatty acids (PUFAs; see scheme). Until recently, PUFAs were known as important metabolites exclusively produced by eukaryotes with oxygen-dependent desaturases. Surprisingly, functional analysis of a PUFA biosynthetic gene cluster from Shewanella sp. strain SCRC2738 points towards a unique mixed pathway involving a polyketide synthase.
Space charge behavior of chemically cross-linked and radiation cross-linked polyethylene (2002)

This paper presents results of an investigation having the aim to compare electrical and oxidative performances of chemically and radiation cross-linked polyethylene. Gamma irradiated and peroxide crosslinked specimens are characterized through dc space charge observations, as well as ac electric strength and oxidation induction time measurements. Similar results, in terms of space charge accumulation and electric strength, are found for specimens having the same gel fraction and obtained by chemical and radiation cross-linking, while longer oxidation times are derived for the latter. This confirms the interest in investigating deeply the possibilities related to gamma radiation crosslinking of polymeric energy cables.
Light transmission in negative curvature hollow core fiber in extremely high material loss region (2013)

In this paper we demonstrate the light transmission in a spectral range of 2.5 to 7.9 µm through a silica negative curvature hollow core fiber (NCHCF) with a cladding consisting of eight capillaries. A separation between the cladding capillaries was introduced to remove the additional resonances in the transmission bands. The measured optical loss at 3.39 µm was about 50 dB/km under a few modes waveguide regime.
Nuclear versus Cytoplasmic Localization of Filamin A in Prostate Cancer: Immunohistochemical Correlation with Metastases (2009)

The data presented in this study indicate that in prostate cancer, metastasis correlates with cytoplasmic localization of FlnA and may be prevented by cleavage and subsequent nuclear translocation of this protein.
A chemical compound commonly used to inhibit PKR, {8‐(imidazol‐4‐ylmethylene)‐6H‐azolidino[5,4‐g] benzothiazol‐7‐one}, protects neurons by inhibiting cyclin‐dependent kinase (2008)

Activation of the double-stranded RNA-dependent protein kinase (PKR) has been implicated in the pathogenesis of several neurodegenerative diseases. We find that a compound widely used as a pharmacological inhibitor of this enzyme, referred to as PKR inhibitor (PKRi), {8-(imidazol-4-ylmethylene)-6H-azolidino[5,4-g]benzothiazol-7-one}, protects against the death of cultured cerebellar granule and cortical neurons. PKRi also prevents striatal neurodegeneration and improves behavioral outcomes in a chemically induced mouse model of Huntington's disease. Surprisingly, PKRi fails to block the phosphorylation of eIF2alpha, a downstream target of PKR, and does not reduce the autophosphorylation of PKR enzyme immunoprecipitated from neurons. Furthermore, neurons lacking PKR are fully protected from apoptosis by PKRi, demonstrating that neuroprotection by this compound is not mediated by PKR inhibition. Using in vitro kinase assays we investigated whether PKRi affects any other protein kinase. These analyses demonstrated that PKRi has no major inhibitory effect on pro-apoptotic kinases such as the c-Jun N-terminal kinases, the p38 MAP kinases and the death-associated protein kinases, or on other kinases including c-Raf, MEK1, MKK6 and MKK7. PKRi does, however, inhibit the activity of certain cyclin-dependent kinases (CDKs), including CDK1, CDK2 and CDK5 both in vitro and in low potassium-treated neurons. Consistent with its inhibitory action on mitotic CDKs, the treatment of HT-22 and HEK293T cell lines with PKRi sharply reduces the rate of cell cycle progression. Taken together with the established role of CDK activation in the promotion of neurodegeneration, our results suggest that PKRi exerts its neuroprotective action by inhibiting CDK.
Radiographic outcome of recent-onset rheumatoid arthritis: A 19-year study of radiographic progression (1998)

Radiographic damage occurs at a constant rate in RA, and is not greater early in RA or reduced later in the course of the illness. Acute-phase reactants are, by far, the strongest determinants of progression.
Differential cis-regulation of human versus mouse TERT gene expression in vivo : Identification of a human-specific repressive element (2005)

In vivo expression of human telomerase is significantly different from that of mouse telomerase. To assess the basis for this difference, a bacterial artificial chromosome clone containing the entire hTERT (human telomerase reverse transcriptase) gene was introduced in mice. In these transgenic mice, expression of the hTERT transgene was similar to that of endogenous hTERT in humans, rather than endogenous mTERT (mouse telomerase reverse transcriptase). In tissues and cells showing a striking difference in expression levels between hTERT in humans and mTERT in mice (i.e., liver, kidney, lung, uterus, and fibroblasts), expression of the hTERT transgene in transgenic mice was repressed, mimicking hTERT in humans. The transcriptional activity of the hTERT promoter was much lower than that of the mTERT promoter in mouse embryonic fibroblasts or human fibroblasts. Mutational analysis of the hTERT and mTERT promoters revealed that a nonconserved GC-box within the hTERT promoter was responsible for the human-specific repression. These results reveal that a difference in cis-regulation of transcription, rather than transacting transcription factors, is critical to species differences in tissue-specific TERT expression. Our data also suggest that the GC-box-mediated, human-specific mechanism for TERT repression is impaired in human cancers. This study represents a detailed characterization of the functional difference in a gene promoter of mice versus humans and provides not only important insight into species-specific regulation of telomerase and telomeres but also an experimental basis for generating mice humanized for telomerase enzyme and its pattern of expression.
Tracheobronchial Pseudomembrane Secondary to Aspergillosis (2006)

Pseudomembrane formation of the tracheobronchus is a unique process noted in a number of disease entities. The significance of this condition needs proper attention as tracheobronchial pseudomembranes have been described throughout most medicine disciplines. We present here a case of tracheobronchial pseudomembrane formation secondary to Aspergillus. A review of the potential etiologies of tracheobronchial pseudomembranes and a relevant review of the array of Aspergillus infections are included.
Preparation and characterization of highly efficient blue TPBI:FIrpic organic light‐emitting devices (2010)

Abstract— Blue phosphorescent organic light‐emitting devices (PhOLEDs) using 1,3,5‐tris(N‐phenyl‐ benzimiazole‐2‐yl)benzene [TPBI] as the host and bis((4,6‐difluorophenyl)‐pyridinate‐N,C2′)picolinate [FIrpic] as the dopant in the emitter were fabricated with different treatments of the hole‐transport layers and doping levels. Among the experimental devices, the best electroluminescent characteristics were obtained in the device with the combined hole‐transport layer of N,N′‐diphenyl‐N,N′‐bis‐[4‐ (phenyl‐m‐tolylamino)‐phenyl]‐biphenyl‐4,4′‐diamine [DNTPD]/1, 1‐bis‐(di‐4‐polyaminophen yl)‐ cyclo‐hexane [TAPC] and a doping level of 10‐vol.% FIrpic. The device with a structure of DNTPD/TAPC/TPBI:Firpic (10%) showed a luminance of 1300 cd/m 2 at an applied voltage of 10 V, a maximum current efficiency of 18 cd/A, and color coordinates of (0.17, 0.43) on the Commission Internationale de I'Eclairage (CIE) chart.
Efficiency of Protein Transduction Is Cell Type-dependent and Is Enhanced by Dextran Sulfate (2002)

Protein transduction domains (PTDs), both naturally occurring and synthetic, have been increasingly utilized to deliver biologically active agents to a variety of cell types in vitro and in vivo. We report that in addition to previously characterized arginine-rich PTDs, including TAT, lysine homopolymers were able to mediate transduction of a wide variety of cell types, as measured by flow cytometric and enzymatic assays. The efficiency of PTD-mediated transduction was influenced by the cell type tested, although polylysine homopolymers demonstrate levels of internalization that consistently exceeded those of TAT and arginine homopolymers. Transduction of arginine/lysine-rich PTDs occurred at 4 degrees C and following depletion of cellular ATP pools, albeit generally at reduced levels. Although transduction was reduced in Chinese hamster ovary mutant lines deficient in either heparan sulfate or glycosaminoglycan synthesis, uptake was restored to wild-type levels by incubating target cells with dextran sulfate. The enhancement of transduction by dextran sulfate suggests that electrostatic interactions play an important first step in the process by which PTDs and their cargo traverse the plasma membrane.
Crystal Statistics. I. A Two-Dimensional Model with an Order-Disorder Transition (1944)

The partition function of a two-dimensional "ferromagnetic" with scalar "spins" (Ising model) is computed rigorously for the case of vanishing field. The eigenwert problem involved in the corresponding computation for a long strip crystal of finite width ($n$ atoms), joined straight to itself around a cylinder, is solved by direct product decomposition; in the special case $n=\ensuremath{\infty}$ an integral replaces a sum. The choice of different interaction energies ($\ifmmode\pm\else\textpm\fi{}J,\ifmmode\pm\else\textpm\fi{}{J}^{\ensuremath{'}}$) in the (0 1) and (1 0) directions does not complicate the problem. The two-way infinite crystal has an order-disorder transition at a temperature $T={T}_{c}$ given by the condition $sinh(\frac{2J}{k{T}_{c}}) sinh(\frac{2{J}^{\ensuremath{'}}}{k{T}_{c}})=1.$ The energy is a continuous function of $T$; but the specific heat becomes infinite as $\ensuremath{-}log |T\ensuremath{-}{T}_{c}|$. For strips of finite width, the maximum of the specific heat increases linearly with $log n$. The order-converting dual transformation invented by Kramers and Wannier effects a simple automorphism of the basis of the quaternion algebra which is natural to the problem in hand. In addition to the thermodynamic properties of the massive crystal, the free energy of a (0 1) boundary between areas of opposite order is computed; on this basis the mean ordered length of a strip crystal is ${(\mathrm{exp} (\frac{2J}{\mathrm{kT}}) tanh(\frac{2{J}^{\ensuremath{'}}}{\mathrm{kT}}))}^{n}.$
A neural feedforward network with a polynomial nonlinearity (2003)

A novel neural network based on the Wiener model is proposed. The network is composed of a hidden layer of preprocessing neurons followed by a polynomial nonlinearity and a linear output neuron. The author tries to solve the problem of finding an appropriate preprocessing method by using a modified backpropagation algorithm. It is shown by the use of calculation trees that the proposed approach is simple to implement, and that the computational complexity is not much larger than for the alternative method of using PCA to determine the weights in the preprocessing network. A simulation is given which indicates superior performance of the proposed network compared to the PCA network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Effects of weak metal-metal interactions in ligand-bridged complexes of ruthenium. Dimeric complexes containing ruthenium ions in different coordination environments (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffects of weak metal-metal interactions in ligand-bridged complexes of ruthenium. Dimeric complexes containing ruthenium ions in different coordination environmentsRobert W. Callahan, Gilbert M. Brown, and Thomas J. MeyerCite this: Inorg. Chem. 1975, 14, 7, 1443–1453Publication Date (Print):July 1, 1975Publication History Published online1 May 2002Published inissue 1 July 1975https://pubs.acs.org/doi/10.1021/ic50149a001https://doi.org/10.1021/ic50149a001research-articleACS PublicationsRequest reuse permissionsArticle Views529Altmetric-Citations103LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts