gebhart/patrepeval huggingface dataset:
The Gene Encoding Ovine Follicle-Stimulating Hormone β: Isolation, Characterization, and Comparison to a Related Ovine Genomic Sequence (1991)

Follicle-stimulating hormone (FSH), the primary stimulus for egg and sperm maturation in mammals, is an alpha/beta heterodimer. Each subunit is encoded by a single-copy gene in the human, bovine, and rat genomes. Transcription of both subunits is inhibited by estradiol and progesterone in ovine pituitary cultures. We report the sequence of one ovine FSH-beta gene (-1,527 to +3,664) that is expressed in vivo and the identification of a novel, second ovine FSH-beta-like sequence. Digestion of ovine genomic DNA with Bgl II yielded two fragments of 10 kb and 15 kb that hybridized to a bovine FSH-beta cDNA. The 10-kb fragment contained 6 kb of 5'-flanking region and all but about 200 bp of the 3' terminus of the ovine FSH-beta gene. This FSH-beta gene encodes a protein that differs from the published ovine protein sequence only at the carboxy terminus (Arg-109Glu-110[STOP codon] instead of Glu-109Arg-110[Glx-111]) and at positions 49 (Ala instead of Thr) and 88 (Arg instead of Ser). This gene is organized similarly to the human, bovine, porcine, and rat FSH-beta genes, and its coding sequence is nearly identical (99.5%) to a reported ovine FSH-beta cDNA. Expression of the FSH-beta gene on the 10-kb fragment in vivo was determined by analysis of wether mRNA using the polymerase chain reaction. A 95-bp sequence of the 15-kb fragment was 87% homologous to the corresponding coding region of the 10-kb fragment. This comparison suggested that the 15-kb fragment contains either an FSH-beta-like sequence or a pseudogene. Several potential steroid response elements were found by sequence analysis of the 5'-flanking region of the FSH-beta gene on the 10-kb fragment. A mechanism by which these elements may act is suggested.
Planning and Drilling Australia's First Medium-Radius Horizontal Wells (1991)

Abstract During early 1991 West Australian Petroleum Pty. Limited acting as joint venture operator for Chevron Asiatic Limited, Texaco Oil Development Company, Shell Development (Australia) Pty. Limited, Ampol Exploration Limited and Western Mining Corporation Limited successfully drilled the first two medium radius wells in Australia. The two wells G88B and Cowle No.2 were very different in many aspects. The first well G88B achieved a 500m horizontal well bore at 615m true vertical depth (TVD) using build up rates to horizontal in excess of 10 degrees/30m(5.818E-03rad/m). The well was drilled onshore using a relatively small drilling rig with minimal additional equipment. The Cowle No.2 well achieved a 1002m horizontal well bore at 1098mTVD using a build up rate of 9 degrees/30m(5.236E-03rad/m). This offshore well utilized a significantly higher capacity/capability drilling rig than that used on the G88B well. Both wells were able to be horizontal within 240m of their surface locations representing a significant decrease in horizontal and vertical displacement required to build to horizontal. Horizontal wells drilled within Australia had previously utilised long radius builds which require greater horizontal and vertical distances before reaching horizontal, longer well paths and generally longer drilling times1. The success of the first two medium radius build to horizontal wells in Australia confirms that horizontal sections can be accurately drilled with shorter horizontal displacements and vertical sections than previously thought possible. The practicality of drilling shallow horizontal wells onshore using available drilling rigs is also confirmed.
A blinded, randomized, placebo‐controlled trial of the safety of lokivetmab (ZTS‐00103289), a caninized anti‐canine IL‐31 monoclonal antibody in client‐owned dogs with atopic dermatitis (2016)

Among a diverse population of 162 client owned dogs with a clinical diagnosis of AD, treatment with two monthly doses of lokivetmab was safe, based on observed adverse events and clinical pathology results over a 42 day period.
Partition of P1 plasmids in Escherichia coli mukB chromosomal partition mutants (1995)

The partition system of the low-copy-number plasmid/prophage of bacteriophage P1 encodes two proteins, ParA and ParB, and contains a DNA site called parS. ParB and the Escherichia coli protein IHF bind to parS to form the partition complex, in which parS is wrapped around ParB and IHF in a precise three-dimensional conformation. Partition can be thought of as a positioning reaction; the plasmid-encoded components ensure that at least one copy of the plasmid is positioned within each new daughter cell. We have used an E. coli chromosomal partition mutant to test whether this positioning is mediated by direct plasmid-chromosomal attachment, for example, by pairing of the partition complex that forms at parS with a bacterial attachment site. The E. coli MukB protein is required for proper chromosomal positioning, so that mukB mutants generate some cells without chromosomes (anucleate cells) at each cell division. We analyzed the plasmid distribution in nucleate and anucleate mukB cells. We found that P1 plasmids are stable in mukB mutants and that they partition into both nucleate and anucleate cells. This indicates that the P1 partition complex is not used to pair plasmids with the host chromosome and that P1 plasmids must be responsible for their own proper cellular localization, presumably through host-plasmid protein-protein interactions.
Ig Heavy Chain Promotes Mature B Cell Survival in the Absence of Light Chain (2007)

Survival of mature B cells is thought to depend on the BCR signaling (BCR) because ablation of either H chain (HC) expression or BCR signaling causes B cells to rapidly disappear. Whether a complete BCR is required for survival of mature B cells is not known. To address this question, we generated a mouse in which we can repress the expression of a transgenic Ig L chain (IgL) by doxycycline (IgL-repressible mouse). Repression of IgL abrogated expression. Surprisingly, however, IgL-negative B cells survived longer than 14 wk, expressed signal-competent HC on the cell's surface, and active unfolded protein response factors. Like postgerminal center B cells, IgL-negative B cells were small lymphocytes, not dividing and expressed Bcl-6. Our results indicate that expression of unpaired HC, as it may occur as a consequence of Ag ligation, somatic hypermutation, or receptor editing, facilitates the survival of cells either by inducing receptor signaling or by inducing unfolded protein response and/or the expression of survival genes such as Bcl-6.
Preliminary X-ray crystallographic analysis of ornithine acetyltransferase (Rv1653) fromMycobacterium tuberculosis (2009)

The gene product of open reading frame Rv1653 from Mycobacterium tuberculosis is annotated as encoding a probable ornithine acetyltransferase (OATase; EC 2.3.1.35), an enzyme that catalyzes two steps in the arginine-biosynthesis pathway. It transfers an acetyl group from N-acetylornithine to L-glutamate to produce N-acetylglutamate and L-ornithine. Rv1653 was crystallized using the sitting-drop vapour-diffusion method. The native crystals diffracted to a resolution of 1.7 A and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 60.1, b = 99.7, c = 155.3 A. The preliminary X-ray study showed the presence of a dimer in the asymmetric unit of the crystals, which had a Matthews coefficient V(M) of 2.8 A(3) Da(-1).
Activation of Flk-1/KDR mediates angiogenesis but not hypotension (1997)

VEGF-induced hypotension is not species specific. It is prevented by nitric oxide inhibition. VEGF-induced angiogenesis and hypotension are not mediated in vivo by the same VEGF receptor.
Molecular Cloning and Characterization of a Novel Gene,CORS26, Encoding a Putative Secretory Protein and Its Possible Involvement in Skeletal Development (2001)

We cloned a novel mouse cDNA, CORS26 (collagenous repeat-containing sequence of 26-kDa protein), encoding a secretory protein by suppression subtractive hybridization between transforming growth factor-beta1-treated and untreated C3H10T1/2 cells. The deduced amino acid sequence of CORS26 consists of 246 amino acids with a secretory signal peptide and contains a collagenous region (Gly-X-Y repeats) at the NH(2) terminus and a complement factor C1q globular domain at the COOH terminus. CORS26 is structurally similar to C1q and to adipocyte-specific protein Acrp30. Transfection analysis suggested that CORS26 is a secretory protein. Northern blot analysis revealed that CORS26 mRNA was present at high levels in rib growth plate cartilage and at moderate levels in kidney of adult mice. CORS26 mRNA was not detected in NIH3T3 cells, BALB/3T3 cells, C3H10T1/2 cells, or osteoblastic MC3T3-E1 cells by reverse transcription-polymerase chain reaction analysis. In situ hybridization of mouse embryos between 13 and 15 days postcoitus revealed relatively high levels of CORS26 mRNA in condensed prechondrocytic cells of cartilage primordia and developing cartilages. However, CORS26 mRNA were undetectable in mature chondrocytes. Furthermore, overexpression of CORS26 enhanced the growth of C3H10T1/2 cells in vitro. The present findings suggest that the CORS26 gene may play an important role in skeletal development.
Thrombin-induced increase in surface expression of epitopes on platelet membrane glycoprotein IIb/IIIa complex and GMP-140 is a function of platelet age (1989)

Platelets are heterogeneous in the content of membrane glycoprotein (GP)IIb/IIIa complex. To determine whether this heterogeneity is related to changes associated with platelet aging in the circulation, newly released platelets, obtained during recovery from nonimmune-mediated acute experimental thrombocytopenia in baboons, were studied. Monoclonal antibody (MoAb) binding to epitopes expressed on GPIIb/IIIa complex (LJ-CP8), GMP-140 (S12), and GPIa/IIa (12F1) was measured on control platelets (comprising platelets with a normal age distribution; mean age 60 to 72 hours) and newly formed platelets (mean age 12 hours), both in the resting state and after thrombin stimulation. Whereas LJ-CP8 binding to resting control platelets increased by 34% upon stimulation by gamma-thrombin from 30,885 +/- 1,171 to 41,458 +/- 1,311 molecules/platelet at saturating concentrations of antibody, LJ-CP8 binding to resting young platelets did not increase significantly upon thrombin stimulation (31,878 +/- 3,330 and 33,791 +/- 3,486 molecules/platelet, respectively). Similarly, binding of antibody S12 in response to maximal thrombin stimulation was reduced by 42% from 10,246 +/- 834 molecules/platelet at saturating concentrations of S12 for control platelets to 5,971 +/- 665 molecules/platelet for young platelets (P = .001). S12 binding to unstimulated platelets was less than 10% of the binding observed after thrombin stimulation at all concentrations of S12 for both control and young platelets. However, maximal binding of antibody 12F1 to resting control platelets did not differ significantly from that observed with resting young platelets (2,926 +/- 167 and 2,857 +/- 208 molecules/platelet, respectively), and 12F1 binding was unchanged after thrombin stimulation for both control and young platelets. We conclude that the thrombin-induced increase in the expression of epitopes on platelet membrane GPIIb/IIIa complex and GMP-140 is a function of platelet age.
Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus- and Species-Specific PCR Primers (2000)

We evaluated the usefulness of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species level. The conservative and species-specific ITS sequences of 33 species of mycobacteria were analyzed in a multialignment analysis. One pair of panmycobacterial primers and seven pairs of mycobacterial species-specific primers were designed. All PCRs were performed under the same conditions. The specificities of the primers were tested with type strains of 20 mycobacterial species from the American Type Culture Collection; 205 clinical isolates of mycobacteria, including 118 Mycobacterium tuberculosis isolates and 87 isolates of nontuberculous mycobacteria from 10 species; and 76 clinical isolates of 28 nonmycobacterial pathogenic bacterial species. PCR with the panmycobacterial primers amplified fragments of approximately 270 to 400 bp in all mycobacteria. PCR with the M. tuberculosis complex-specific primers amplified an approximately 120-bp fragment only for the M. tuberculosis complex. Multiplex PCR with the panmycobacterial primers and the M. tuberculosis complex-specific primers amplified two fragments that were specific for all mycobacteria and the M. tuberculosis complex, respectively. PCR with M. avium complex-, M. fortuitum-, M. chelonae-, M. gordonae-, M. scrofulaceum-, and M. szulgai-specific primers amplified specific fragments only for the respective target organisms. These novel primers can be used to detect and identify mycobacteria simultaneously under the same PCR conditions. Furthermore, this protocol facilitates early and accurate diagnosis of mycobacteriosis.
Query suggestion using hitting time (2008)

Generating alternative queries, also known as query suggestion, has long been proved useful to help a user explore and express his information need. In many scenarios, such suggestions can be generated from a large scale graph of queries and other accessory information, such as the clickthrough. However, how to generate suggestions while ensuring their semantic consistency with the original query remains a challenging problem.
Environmental Temperatures Influence Antioxidant Properties and Mineral Content in Broccoli Cultivars Grown Organically and Conventionally (2011)

Broccoli (Brassica oleracea L.) is a widely consumed cool-season crop that is well-known for its health benefits and high nutritional content. With a growing consumer interest in local and organic foods, it would be helpful to determine if farmers can improve the nutrient and antioxidant content of broccoli through cultivar selection and to gain insight into environmental effects on nutritional properties. This study evaluated total phenolic content (TPC), antioxidant capacity (ABTS.+ assay), and ascorbic acid (AA), as well as the levels of ten nutrients (N, P, K, S, Ca, Mg, Zn, Fe, Mn, and Cu) in six broccoli cultivars ('Arcadia,' 'Emerald Pride,' 'Gypsy,' 'Packman,' 'Premium Crop,' and 'SBC8410'). Six cultivars were grown in Northern Colorado for two years using conventional and certified organic practices. Production year had a significant effect (p<0.05) on both antioxidant levels and nutrient levels, perhaps a result of warmer temperatures reflected by quite different weather patterns between the two growing years. Nutrient levels were higher in 2005, a cool production season, while antioxidant levels were higher in 2006 in a warm season (p<0.0001). Organic broccoli had overall higher levels for TPC, antioxidant capacity, P, K, and Ca, while conventional broccoli was higher in Fe (p<0.05). Significant cultivar differences were found in most tests. All three antioxidant tests were positively correlated (p<0.05) with each other and negatively correlated with N, P, K, S, Ca, Mg, Zn, Mn, and Cu levels, indicating an inverse relationship between antioxidant levels and mineral content. This study suggests that choice of cultivar provides a viable option for local farmers who may wish to market more nutritious broccoli. Additional work is needed to confirm the preliminary indication that warm temperatures may trigger elevated antioxidant properties, but lower mineral uptake.
Microenvironmental VEGF concentration, not total dose, determines a threshold between normal and aberrant angiogenesis (2004)

Use of long-term constitutive expression of VEGF for therapeutic angiogenesis may be limited by the growth of abnormal blood vessels and hemangiomas. We investigated the relationship between VEGF dosage and the morphology and function of newly formed blood vessels by implanting retrovirally transduced myoblasts that constitutively express VEGF164 into muscles of adult mice. Reducing VEGF dosage by decreasing the total number of VEGF myoblasts implanted did not prevent vascular abnormalities. However, when clonal populations of myoblasts homogeneously expressing different levels of VEGF were implanted, a threshold between normal and aberrant angiogenesis was found. Clonal myoblasts that expressed low to medium levels of VEGF induced growth of stable, pericyte-coated capillaries of uniform size that were not leaky and became VEGF independent, as shown by treatment with the potent VEGF blocker VEGF-TrapR1R2. In contrast, clones that expressed high levels of VEGF induced hemangiomas. Remarkably, when different clonal populations were mixed, even a small proportion of cells with high production of VEGF was sufficient to cause hemangioma growth. These results show for the first time to our knowledge that the key determinant of whether VEGF-induced angiogenesis is normal or aberrant is the microenvironmental amount of growth factor secreted, rather than the overall dose. Long-term continuous delivery of VEGF, when maintained below a threshold microenvironmental level, can lead to normal angiogenesis without other exogenous growth factors.
Syntheses, properties, and structures of anhydrous tetramethylammonium fluoride and its 1:1 adduct with trans-3-amino-2-butenenitrile (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSyntheses, properties, and structures of anhydrous tetramethylammonium fluoride and its 1:1 adduct with trans-3-amino-2-butenenitrileKarl O. Christe, William W. Wilson, Richard D. Wilson, Robert Bau, and Jin An FengCite this: J. Am. Chem. Soc. 1990, 112, 21, 7619–7625Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October 1990https://pubs.acs.org/doi/10.1021/ja00177a025https://doi.org/10.1021/ja00177a025research-articleACS PublicationsRequest reuse permissionsArticle Views3926Altmetric-Citations270LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development (2007)

Neuronal gene expression is tightly regulated in developing CNS. Here, we demonstrate the anti-neural function of phosphatase SCP1 (small C-terminal domain phosphatase 1) during development. We further show that the neuron-enriched microRNA miR-124 directly targets SCP1-3' untranslated region (UTR) to suppress SCP1 expression. In developing spinal cord, expression of miR-124 and SCP1 is complementary, and miR-124 antagonism phenocopies SCP1 overexpression and vice versa. In P19 cells, miR-124 suppresses SCP1 expression and induces neurogenesis, and SCP1 counteracts this proneural activity of miR-124. Our results suggest that, during CNS development, timely down-regulation of SCP1 is critical for inducing neurogenesis, and miR-124 contributes to this process at least in part by down-regulating SCP1 expression.
Mapping the Fan Locus Controlling Linolenic Acid Content in Soybean Oil (1995)

Journal Article Mapping the Fan Locus Controlling Linolenic Acid Content in Soybean Oil Get access E. C. Brummer, E. C. Brummer Department of Agronomy, Iowa State UniversityAmes, IA 50011 Search for other works by this author on: Oxford Academic PubMed Google Scholar A. D. Nickell, A. D. Nickell The Asgrow Seed CompanyJanesville, Wisconsin Search for other works by this author on: Oxford Academic PubMed Google Scholar J. R. Wilcox, J. R. Wilcox USDAARS and Department of Agronomy, Purdue UniversityWest Lafayette, Indiana Search for other works by this author on: Oxford Academic PubMed Google Scholar R. C. Shoemaker R. C. Shoemaker USDA-ARSFCR, Departments of Agronomy and Zoology and Genetics, Iowa State UniversityAmes Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Heredity, Volume 86, Issue 3, May 1995, Pages 245–247, https://doi.org/10.1093/oxfordjournals.jhered.a111572 Published: 01 May 1995 Article history Received: 29 July 1994 Accepted: 18 November 1994 Published: 01 May 1995
Proton Pump (H+/K+‐ATPase) Expression in Human Laryngeal Seromucinous Glands (2005)

B-3.
Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction (1990)

A two-step polymerase chain reaction (PCR) procedure was used as a new screening strategy for the detection of human papillomavirus (HPV) genotypes in cervical scrapes omitting prior DNA extraction. Sample preparation consisted of a freeze-thaw step followed by boiling the cells before the PCR mixture was added. This pretreatment was as efficient and reproducible for HPV DNA amplification as DNA purification. By using crude cell suspensions, a prescreening of the samples with the general primer-mediated PCR method (GP-PCR) was performed to detect a broad spectrum of sequenced and still unsequenced HPV types at the subpicogram level. HPV-containing scrapes by GP-PCR were subjected to HPV 6, 11, 16, 18, 31, and 33 type-specific PCR (TS-PCR) to identify the sequenced HPV types. This direct GP/TS-PCR method was tested on a large group of cervical scrapes (n = 459) from women visiting a gynecologic outpatient clinic. The results were compared with HPV data obtained by a method using modified filter in situ hybridization and TS-PCR in which the PCR was mainly used to confirm HPV positivity. A substantially higher HPV prevalence rate was found by direct GP/TS-PCR strategy. The results indicate that GP/TS-PCR is a rapid, sensitive, and reliable detection method for HPV in cervical scrapes. The easy performance on crude cell suspensions makes this strategy applicable for large HPV-screening programs.