gebhart/patrepeval huggingface dataset:
Time-selective signaling and reception for communication over multipath fading channels (2000)

The mobile wireless channel affords inherent diversity to combat the effects of fading. Existing code-division multiple-access systems, by virtue of spread-spectrum signaling and RAKE reception, exploit only part of the channel diversity via multipath combination. Moreover, their performance degrades under fast fading commonly encountered in mobile scenarios. In this paper, we develop new signaling and reception techniques that maximally exploit channel diversity via joint multipath-Doppler processing. Our approach is based on a canonical representation of the wireless channel, which leads to a time-frequency generalization of the RAKE receiver for diversity processing. Our signaling scheme facilitates joint multipath-Doppler diversity by spreading the symbol waveform beyond the intersymbol duration to make the channel time-selective. A variety of detection schemes are developed to account for the intersymbol interference (ISI) due to overlapping symbols. However, our results indicate that the effects of ISI are virtually negligible due to the excellent correlation properties of the pseudorandom codes. Performance analysis also shows that relatively small Doppler spreads can yield significant diversity gains. The inherently higher level of diversity achieved by time-selective signaling brings the fading channel closer to an additive white Gaussian noise channel, thereby facilitating the use of powerful existing coding techniques for Gaussian channels.
The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis (2003)

Rubber biosynthesis takes place on the surface of rubber particles. These particles are surrounded by a monolayer membrane in which the rubber transferase is anchored. In order to gain better insight into whether rubber particles from different plant species share common structural characteristics, the micromorphology of rubber particles from Ficus carica, Ficus benghalensis, and Hevea brasiliensis was examined by electron microscopy. Rubber particles of all three species were spherical in shape, and the size of rubber particles of H. brasiliensis was much smaller than those of F. carica and F. benghalensis. In addition, investigations were undertaken to compare the cross-reactivity of the antibody raised against either the H. brasiliensis small rubber particle protein (SRPP) which is suggested to be involved in rubber biosynthesis, or the cis-prenyltransferase (CPT) which has an activity similar to rubber transferase. Both western analysis and TEM-immunogold labelling studies showed that rubber particles of F. carica and F. benghalensis do not contain the SRPP. None of the rubber particles in F. carica, F. benghalensis and H. brasiliensis contained the CPT, suggesting that the CPT itself could not catalyse the formation of high molecular weight rubber. These results indicate that rubber particles in the three different plant species investigated share some degree of similarity in architecture, and that the SRPP and CPT themselves are not the core proteins necessary for rubber biosynthesis.
Human Enteric Defensins (1996)

Paneth cells, secretory epithelial cells of the small intestinal crypts, are proposed to contribute to local host defense. Both mouse and human Paneth cells express a collection of antimicrobial proteins, including members of a family of antimicrobial peptides named defensins. In this study, data from an anchored polymerase chain reaction (PCR) strategy suggest that only two defensin mRNA isoforms are expressed in the human small intestine, far fewer than the number expressed in the mouse. The two isoforms detected by this PCR approach were human defensin family members, HD-5 and HD-6. The gene encoding HD-6 was cloned and characterized. HD-6 has a genomic organization similar to HD-5, and the two genes have a striking pattern of sequence similarity localized chiefly in their proximal 5'-flanking regions. Analysis of human fetal RNA by reverse transcriptase-PCR detected enteric defensin HD-5 mRNA at 13.5 weeks of gestation in the small intestine and the colon, but by 17 weeks HD-5 was restricted to the small intestine. HD-6 mRNA was detectable at 13.5-17 weeks of gestation in the small intestine but not in the colon. This pattern of expression coincides with the previously described appearance of Paneth cells as determined by ultrastructural approaches. Northern analysis of total RNA from small intestine revealed quantifiable enteric defensin mRNA in five samples from 19 24 weeks of gestation at levels approximately 40-250-fold less than those observed in the adult, with HD-5 mRNA levels greater than those of HD-6 in all samples. In situ hybridization analysis localized expression of enteric defensin mRNA to Paneth cells at 24 weeks of gestation, as is seen in the newborn term infant and the adult. Consistent with earlier morphological studies, the ratio of Paneth cell number per crypt was reduced in samples at 24 weeks of gestation compared with the adult, and this lower cell number partially accounts for the lower defensin mRNA levels as determined by Northern analysis. Low levels of enteric defensin expression in the fetus may be characteristic of an immaturity of local defense, which is thought to predispose infants born prematurely to infection from intestinal microorganisms.
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR) (1989)

Journal Article Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR) Get access Matthias Kammann, Matthias Kammann Max-Planck-Institut fflr Zuchtungsforschung5000 K61n 30, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Järgen Laufs, Järgen Laufs Max-Planck-Institut fflr Zuchtungsforschung5000 K61n 30, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Jeff Schell, Jeff Schell Max-Planck-Institut fflr Zuchtungsforschung5000 K61n 30, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Bruno Gronenbom Bruno Gronenbom Max-Planck-Institut fflr Zuchtungsforschung5000 K61n 30, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 17, Issue 13, 11 July 1989, Page 5404, https://doi.org/10.1093/nar/17.13.5404 Published: 11 July 1989 Article history Received: 05 June 1989 Published: 11 July 1989
Smartphone based health accessory for colorimetric detection of biomarkers in sweat and saliva (2013)

The mobile health market is rapidly expanding and portable diagnostics tools offer an opportunity to decrease costs and increase the availability of healthcare. Here we present a smartphone based accessory and method for the rapid colorimetric detection of pH in sweat and saliva. Sweat pH can be correlated to sodium concentration and sweat rate in order to indicate to users the proper time to hydrate during physical exercise and avoid the risk of muscle cramps. Salivary pH below a critical threshold is correlated with enamel decalcification, an acidic breakdown of calcium in the teeth. We conduct a number of human trials with the device on a treadmill to demonstrate the ability to monitor changes in sweat pH due to exercise and electrolyte intake and predict optimal hydration. Additionally, we perform trials to measure salivary pH over time to monitor the effects of diet on oral health risks.
Efficacy and Safety of Vamorolone vs Placebo and Prednisone Among Boys With Duchenne Muscular Dystrophy (2022)

ClinicalTrials.gov Identifier: NCT03439670.
Heteroplasmic Point Mutations of Mitochondrial DNA Affecting Subunit I of Cytochrome c Oxidase in Two Patients With Acquired Idiopathic Sideroblastic Anemia (1997)

Mitochondrial iron overload in acquired idiopathic sideroblastic anemia (AISA) may be attributable to mutations of mitochondrial DNA (mtDNA), because these can cause respiratory chain dysfunction, thereby impairing reduction of ferric iron (Fe3+) to ferrous iron (Fe2+). The reduced form of iron is essential to the last step of mitochondrial heme biosynthesis. It is not yet understood to which part of the respiratory chain the reduction of ferric iron is linked. In two patients with AISA we identified point mutations of mtDNA affecting the same transmembrane helix within subunit I of cytochrome c oxidase (COX I; ie, complex IV of the respiratory chain). The mutations were detected by restriction fragment length polymorphism analysis and temperature gradient gel electrophoresis. One of the mutations involves a T --> C transition in nucleotide position 6742, causing an amino acid change from methionine to threonine. The other mutation is a T --> C transition at nt 6721, changing isoleucine to threonine. Both amino acids are highly conserved in a wide range of species. Both mutations are heteroplasmic, ie, they establish a mixture of normal and mutated mitochondrial genomes, which is typical of disorders of mtDNA. The mutations were present in bone marrow and whole blood samples, in isolated platelets, and in granulocytes, but appeared to be absent from T and B lymphocytes purified by immunomagnetic bead separation. They were not detected in buccal mucosa cells obtained by mouthwashes and in cultured skin fibroblasts examined in one of the patients. In both patients, this pattern of involvement suggests that the mtDNA mutation occurred in a self-renewing bone marrow stem cell with myeloid determination. Identification of two point mutations with very similar location suggests that cytochrome c oxidase plays an important role in the pathogenesis of AISA. COX may be the physiologic site of iron reduction and transport through the inner mitochondrial membrane.
Non-invasive in-vivo temperature mapping of ultrasound heating using fluorine-based magnetic resonance imaging agents (2002)

The aim of this study was to investigate the feasibility of using fluorine based magnetic resonance to measure accurately hepatic heating from a focused ultrasound transducer in rats. This new method uses the temperature dependence of the difference in fluorine chemical shifts within a single molecule to provide internal compensation for complicating physiological effects. Female Sprague-Dawley rats were exposed to 5 minutes of CW irradiation at 1.37 MHz from a focused transducer. Theoretical calculations based on the monopole source solution to the bio-heat transfer equation gave a volume-averaged temperature rise of 2.1/spl deg/C. Preliminary experiments using protein encapsulated perfluorooctylbromide targeted to the liver gave an empirical rise of 2.0/spl plusmn/0.4/spl deg/C.
Real time face and object tracking as a component of a perceptual user interface (2002)

As a step towards a perceptual user interface, an object tracking algorithm is developed and demonstrated tracking human faces. Computer vision algorithms that are intended to form part of a perceptual user interface must be fast and efficient. They must be able to track in real time and yet not absorb a major share of computational resources. An efficient, new algorithm is described here based on the mean shift algorithm. The mean shift algorithm robustly finds the mode (peak) of probability distributions. We first describe histogram based methods of producing object probability distributions. In our case, we want to track the mode of an object's probability distribution within a video scene. Since the probability distribution of the object can change and move dynamically in time, the mean shift algorithm is modified to deal with dynamically changing probability distributions. The modified algorithm is called the Continuously Adaptive Mean Shift (CAMSHIFT) algorithm. CAMSHIFT is then used as an interface for games and graphics.
Preservation of platelet responsiveness to nitroglycerine despite development of vascular nitrate tolerance (2005)

Chronic therapy with either ISMN or TD-NTG is associated with development of vascular tolerance. Despite the induction of vascular tolerance, platelet responsiveness to NTG and SNP remains unaffected. Therefore, development of vascular tolerance is unlikely to compromise the anti-aggregatory effects of organic nitrates, or those of endogenous NO.
Comparative Analysis of Physicochemical and Immunochemical Properties of the Two Major Allergens from <i>Dermatophagoides pteronyssinus </i>and the Corresponding Allergens from <i>Dermatophagoides farinae</i> (1989)

Two major allergens, DP1 (Der p I) and DP2 (Der p II), were isolated from the whole culture extract of Dermatophagoides pteronyssinus, and the physicochemical and immunochemical properties of these allergens were compared with those of the corresponding allergens from Dermatophagoides farinae, DF1 (Der fI) and DF2 (Der fII). On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, polyacrylamide gel isoelectric focusing and amino acid analysis, both DP1 and DP2 were demonstrated to have close physicochemical similarity with DF1 and DF2, respectively. On immunodiffusion with the use of rabbit antisera, the two Der I allergens showed the reaction of typical partial identity, while the two Der II allergens showed the reaction of almost complete identity. Radioallergosorbent test (RAST) and RAST absorption experiments with the use of sera from mite-allergic patients showed that human IgE antibody response to the Der I allergens was directed against both cross-reactive and species-specific determinants. In contrast, IgE antibodies to the Der II allergens were demonstrated to react almost completely to cross-reactive determinants.
Multiple roles of the vesicular‐SNARE TI‐VAMP in post‐Golgi and endosomal trafficking (2009)

SNARE (Soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are the core machinery of membrane fusion. Vesicular SNAREs (v-SNAREs) interact with their target SNAREs (t-SNAREs) to form SNARE complexes which mediate membrane fusion. Here we review the basic properties and functions of the v-SNARE TI-VAMP/VAMP7 (Tetanus neurotoxin insensitive-vesicle associated membrane protein). TI-VAMP interacts with its t-SNARE partners, particularly plasmalemmal syntaxins, to mediate membrane fusion and with several regulatory proteins especially via its amino-terminal regulatory Longin domain. Partners include AP-3, Hrb/(Human immunodeficiency virus Rev binding) protein, and Varp (Vps9 domain and ankyrin repeats containing protein) and regulate TI-VAMP's function and targeting. TI-VAMP is involved both in secretory and endocytic pathways which mediate neurite outgrowth and synaptic transmission, plasma membrane remodeling and lysosomal secretion.
NACP, the precursor protein of the non-amyloid beta/A4 protein (A beta) component of Alzheimer disease amyloid, binds A beta and stimulates A beta aggregation. (1995)

NACP, a 140-amino acid presynaptic protein, is the precursor of NAC [the non-amyloid beta/A4 protein (A beta) component of Alzheimer disease (AD) amyloid], a peptide isolated from and immunologically localized to brain amyloid of patients afflicted with AD. NACP produced in Escherichia coli bound to A beta peptides, the major component of AD amyloid. NACP bound to A beta 1-38 and A beta 25-35 immobilized on nitrocellulose but did not bind to A beta 1-28 on the filter under the same conditions. NACP binding to A beta 1-38 was abolished by addition of A beta 25-35 but not by A beta 1-28, suggesting that the hydrophobic region of the A beta peptide is critical to this binding. NACP-112, a shorter splice variant of NACP containing the NAC sequence, bound to A beta, but NACP delta, a deletion mutant of NACP lacking the NAC domain, did not bind A beta 1-38. Furthermore, binding between NACP-112 and A beta 1-38 was decreased by addition of peptide Y, a peptide that covers the last 15 residues of NAC. In an aqueous solution, A beta 1-38 aggregation was observed when NACP was also present in an incubation mixture at a ratio of 1:125 (NACP/A beta), whereas A beta 1-38 alone or NACP alone did not aggregate under the same conditions, suggesting that the formation of a complex between A beta and NACP may promote aggregation of A beta. Thus, NACP can bind A beta peptides through the specific sequence and can promote A beta aggregation, raising the possibility that NACP may play a role in the development of AD amyloid.
Lipoprotein(a): from ancestral benefit to modern pathogen? (2000)

We review current concepts regarding the genetic, structural and metabolic features of lipoprotein(a), a major inherited cardiovascular pathogen. Although lipoprotein(a) is almost completely confined to a subset of primates, the hedgehog produces a lipoprotein(a)-like complex, which appears to have evolved independently from that of humans. The physiological role of lipoprotein(a) in humans is still unclear, and individuals with low or null concentrations of plasma lipoprotein(a) manifest no deficiency syndrome or disease. The integration of recent discoveries about the structure and metabolism of this unique lipoprotein particle has allowed the formulation of some hypotheses concerning the evolutionary advantages of synthesizing lipoprotein(a)-like particles.
Basal Plus Basal–Bolus Approach in Type 2 Diabetes (2011)

Type 2 diabetes is characterized by insulin resistance and progressive β-cell deterioration. As β-cell function declines, most patients with type 2 diabetes treated with oral agents, in monotherapy or combination, will require insulin therapy. Addition of basal insulin (glargine, detemir, or NPH/neutral protamine lispro insulin) to previous treatment is accepted as the simplest way to start insulin therapy in those patients. But even when basal insulin is adequately titrated, some patients will also need prandial insulin to achieve or maintain individual glycemic targets over time. Starting with premixed insulin is an effective option, but it is frequently associated with increased hypoglycemia risk, fixed meal schedules, and weight gain. As an alternative, a novel approached known as "basal plus strategy" has been developed. This approach considers the addition of increasing injections of prandial insulin, beginning with the meal that has the major impact on postprandial glucose values. Finally, if this is not enough intensification to basal-bolus will be necessary. In reducing hyperglycemia, this modality still remains the most effective option, even in people with type 2 diabetes. This article will review the currently evidence on the basal plus strategy and also its progression to basal-bolus therapy. In addition, practical recommendations to start and adjust basal plus therapy will be provided.
16 alpha-[77Br]bromo-11 beta-methoxyestradiol-17 beta: a gamma-emitting estrogen imaging agent with high uptake and retention by target organs. (1982)

16 alpha[77Br]Bromo-11 beta-methoxyestradiol-17 beta [MBE(Br-77)], a compound with high affinity for the estrogen receptor and with low nonspecific binding, has been prepared with an effective specific activity of 770--1450 Ci per mmole at the time of synthesis. In immature female rats, this compound is taken up selectively by the uterus and is retained for prolonged periods. This is presumably due to the binding of this compound to the estrogen receptor, as uterine uptake is blocked selectively by coadministration of an excess of unlabeled estradiol, and administration of a chase dose of unlabeled estradiol results in a rapid decrease in activity in the uterus. In double-label experiments with 16 alpha[125I]estradiol and MBE(Br-77), the two compounds showed equally selective uterine uptake at 1 hr, but the bromine-labeled compound became increasingly more selective at 3 and 6 hr. MBE(Br-77) may prove to be a more favorable agent for imaging human breast tumors than our previously described compound, 16 alpha-[77Br]bromoestradiol-17 beta.
Recognizing Sepsis as a Global Health Priority — A WHO Resolution (2017)

Jena Univ Hosp, Jena, Germany
Solid phase microextraction (SPME)-transmission mode (TM) pushes down detection limits in direct analysis in real time (DART) (2014)

A new SPME device was developed and applied for quick solventless extraction/enrichment of small molecules from complex matrices. Subsequently, the device was coupled as a transmission mode substrate to DART resulting in limits of detection in the low pg mL(-1) level in less than 3 minutes with reproducibility below 5% RSD.
LimitLESS Directories: A Scalable Cache Coherence Scheme (1991)

Caches enhance the performance of multiprocessors by reducing network tra c and average memory access latency.However, cache-based systems must address the problem of cache coherence.We propose the LimitLESS directory protocol to solve this problem.The LimitLESS scheme uses a combination of hardware and software techniques to realize the performance of a full-map directory with the memory overhead of a limited directory.This protocol is supported by Alewife, a large-scale multiprocessor.We describe the architectural interfaces needed to implement the LimitLESS directory, and evaluate its performance through simulations of the Alewife machine.
Hydrolysis Kinetics of Astaxanthin Esters and Stability of Astaxanthin of Haematococcus pluvialis during Saponification (1998)

The reaction kinetics for the hydrolysis of astaxanthin esters and the degradation of astaxanthin during saponification of the pigment extract from the microalga Haematococcus pluvialis were investigated. Different concentrations of sodium hydroxide in methanol were used for the saponification under nitrogen in darkness at ambient temperature (22 degrees C) followed by the analysis of astaxanthins and other carotenoids using an HPLC method. The concentration of methanolic NaOH solution was important for promoting the hydrolysis of astaxanthin esters and minimizing the degradation of astaxanthin during saponification. With a higher concentration of methanolic NaOH solution, the reaction rate of hydrolysis was high, but the degradation of astaxanthin occurred significantly. The rate constants of the hydrolysis reaction (first order) of astaxanthin esters and the degradation reaction (zero-order) of astaxanthin were directly proportional to the concentration of sodium hydroxide in the saponified solution. Although the concentration of sodium hydroxide in the saponified solution was 0.018 M, complete hydrolysis of astaxanthin esters was achieved in 6 h for different concentrations (10-100 mg/L) of pigment extracts. Results also indicated that a higher temperature should be avoided to minimize the degradation of astaxanthin. In addition, during saponification, no loss of lutein, beta-carotene, and canthaxanthin was found.
705. Reactions of Nitroparaffins. Part III. The reaction of primary Nitroparaffins with acetic anhydride (1949)

T. Urbański, J. Chem. Soc., 1949, 3374 DOI: 10.1039/JR9490003374
Direct Serum Total Iron-binding Capacity Assay Suitable for Automated Analyzers (2002)

The new DTIBC assay is suitable for routine use in clinical laboratories and may improve the quality of iron metabolism studies.
REDUCTION OF HETEROCYCLIC ALCOHOLS WITH SODIUM BOROHYDRIDE-TRIFLUOROACETIC ACID. PREPARATION OF bis-HETEROCYCLIC METHANES (1991)

(1991). REDUCTION OF HETEROCYCLIC ALCOHOLS WITH SODIUM BOROHYDRIDE-TRIFLUOROACETIC ACID. PREPARATION OF bis-HETEROCYCLIC METHANES. Organic Preparations and Procedures International: Vol. 23, No. 4, pp. 403-411.