gebhart/patrepeval huggingface dataset:
The effect of P2Y-mediated platelet activation on the release of VEGF and endostatin from platelets (2010)

Vascular endothelial growth factor (VEGF) and endostatin are key protein modulators of angiogenesis found within platelets. The platelet activation pathways that control angiogenic protein release are incompletely elucidated. The differential release of pro-angiogenic and anti-angiogenic proteins from the platelet has been demonstrated for proteinase activated receptors (PARs). Given the ability of tumors to secrete ADP and the availability of ADP receptor antagonists clinically, we determined the influence of adenosine diphosphate (ADP) and the ADP receptors, P2Y(1) and P2Y(12), on platelet release of the angiogenic stimulator protein, VEGF, and the angiogenic inhibitor protein, endostatin. Minimally altered whole blood (WB) and platelet rich plasma (PRP) from healthy volunteers was stimulated with ADP alone (12.5 uM), in combination with a P2Y(1) antagonist (MRS2179) or a P2Y(12) antagonist (cangrelor). VEGF and endostatin protein concentrations were assessed by an ELISA assay. We report that maximally stimulating concentrations of ADP significantly increased VEGF release from platelets in both PRP and WB by 36+/-12% 36+/-12% 54+/-18% 36 +/- 12% (p < 0.05) respectively as compared to control. Both P2Y(1) and P2Y(12) receptor antagonism inhibited this release. Conversely, endostatin levels did not change following ADP stimulation in PRP, while a 4.7% (p = 0.03) increase was observed in WB. As compared to thrombin receptor activation, ADP activation was a weaker stimulus for VEGF release. We found that activation of platelets by ADP results in an increase in soluble VEGF concentrations with minimal effects on endostatin concentrations, suggesting ADP release in the tumor microenvironment may be, on balance, proangiogenic. P2Y receptor antagonism abrogates ADP mediated proangiogenic protein release and thus may represent a potential pharmacologic strategy for regulating platelet mediated angiogenesis.
Biomarker discovery with SELDI-TOF MS in human urine associated with early renal injury: evaluation with computational analytical tools (2007)

Our results substantiate the effectiveness of Seldi-TOF MS-based computational analysis as a tool for discovering potential biomarkers in urine samples associated with early renal injury.
Trends in Nanomaterial-Based Non-Invasive Diabetes Sensing Technologies (2014)

Blood glucose monitoring is considered the gold standard for diabetes diagnostics and self-monitoring. However, the underlying process is invasive and highly uncomfortable for patients. Furthermore, the process must be completed several times a day to successfully manage the disease, which greatly contributes to the massive need for non-invasive monitoring options. Human serums, such as saliva, sweat, breath, urine and tears, contain traces of glucose and are easily accessible. Therefore, they allow minimal to non-invasive glucose monitoring, making them attractive alternatives to blood measurements. Numerous developments regarding noninvasive glucose detection techniques have taken place over the years, but recently, they have gained recognition as viable alternatives, due to the advent of nanotechnology-based sensors. Such sensors are optimal for testing the amount of glucose in serums other than blood thanks to their enhanced sensitivity and selectivity ranges, in addition to their size and compatibility with electronic circuitry. These nanotechnology approaches are rapidly evolving, and new techniques are constantly emerging. Hence, this manuscript aims to review current and future nanomaterial-based technologies utilizing saliva, sweat, breath and tears as a diagnostic medium for diabetes monitoring.
Photoaffinity Labeling by 4-Thiodideoxyuridine Triphosphate of the HIV-1 Reverse Transcriptase Active Site during Synthesis (1998)

The active site of HIV-1 reverse transcriptase (HIV-1 RT) was investigated by photoaffinity labeling based on catalytic competence. A stable ternary elongation complex was assembled containing enzyme, DNA template (RT20), DNA primer molecule (P12), and the necessary dNTPs (one of which was alpha-32P-labeled) needed for primer elongation. The photoaffinity probe 4-thiodideoxyuridine triphosphate was incorporated uniquely at the 3' terminus of the 32P-labeled DNA product. Upon photolysis, the p66 subunit of a HIV-1 RT heterodimer (p66/p51) was uniquely cross-linked to the DNA product and subsequently digested by either trypsin or endoproteinase Lys-C. The labeled HIV-1 RT peptide was separated, purified, and finally subjected to Edman microsequencing. A unique radioactive hexapeptide (V276RQLCK281) was identified and sequenced. Our photoaffinity labeling results were positioned on the HIV-1 RT. DNA.Fab complex x-ray crystallography structure and compared with the suggested aspartic triad active site.
Cloning and expression of cDNA for arginine-specific ADP-ribosyltransferase from chicken bone marrow cells. (1994)

Two arginine-specific ADP-ribosyltransferase cDNAs (designated AT1 and AT2) were cloned from chicken bone marrow cells. Each cDNA encodes a different peptide of 312 amino acid residues. Homology of deduced amino acid sequences between AT1 and AT2 was 78.3%. We found all six combined peptide sequences of 222 amino acid residues derived from purified chicken heterophil ADP-ribosyltransferase (Mishima, K., Terashima, M., Obara, S., Yamada, K., Imai, K., and Shimoyama, M. (1991) J. Biochem. (Tokyo) 110, 388-394) in the deduced amino acid sequence of AT1, with two amino acid mismatches. Arginine-specific ADP-ribosyltransferase activity was detected in culture medium of COS 7 cells transiently transfected with AT1 cDNA, while activity from the cells transfected with AT2 cDNA was found in both culture medium and cell lysate. AT1 transferase required 2-mercaptoethanol for the activity. The activity was inhibited in the presence of NaCl while AT2 enzyme was activated by either agent. On zymographic in situ gel analysis, estimated molecular masses of the AT1, AT2 and purified chicken heterophil transferases were 32, 34, and 27.5 kDa, respectively. Northern blot analysis with specific probes to AT1 or AT2 cDNAs revealed about a 1.5-kilobase message in chicken bone marrow cells but no signals were observed in heterophils, spleen, and liver of chicken or human HL-60 cells. Highly conserved regions were observed among the deduced amino acid sequences of AT1, AT2, rabbit skeletal muscle transferase, and rodent T-cell surface antigen RT6s.
Las reformas procesales para la implantación de la nueva oficina judicial en el proceso declarativo civil (2009)

La nueva organizacion de la oficina judicial merece una valoracion positiva. Habia sido demandada por todos los protagonistas de la Justicia y ahora se trata de verificar una adecuada implantacion que conceda a cada uno la responsabilidad que le compete, pero manteniendo siempre el sentido instrumental de la reforma, es decir, que la oficina judicial sea la mas perfecta organizacion al servicio del acto jurisdiccional de juzgar y hacer ejecutar lo juzgado, mandato constitucional exigido a los jueces y magistrados. Los proyectos de reformas procesales que se analizan pretenden acomodar las leyes procesales vigentes a ese nuevo reparto funcional entre jueces y secretarios judiciales.
Supramolecular Self-Assembly of Lipid Derivatives on Carbon Nanotubes (2003)

Images of the assembly of surfactants and synthetic lipids on the surface of carbon nanotubes were obtained by transmission electron microscopy. Above the critical micellar concentration, sodium dodecyl sulfate (SDS) forms supramolecular structures made of rolled-up half-cylinders on the nanotube surface. Depending on the symmetry and the diameter of the carbon nanotube, we observed rings, helices, or double helices. Similar self-assemblies were also obtained with several synthetic single-chain lipids designed for the immobilization of histidine-tagged proteins. At the nanotube-water interface, permanent assemblies were produced from mixed micelles of SDS and different water-insoluble double-chain lipids after dialysis of the surfactant. Such arrangements could be further exploited for the development of new biosensors and bioelectronic nanomaterials.
Brace Wear Control of Curve Progression in Adolescent Idiopathic Scoliosis (2010)

The Boston brace is an effective means of controlling curve progression in patients with adolescent idiopathic scoliosis when worn for more than twelve hours per day.
Comparative Terminal Realizations with Alpha-Geometric Coding (1980)

The introduction of videotex services in several countries has spurred the search for the lowest cost user terminal which will provide acceptable performance. This has led to many arguments on the quality of graphics required. One faction argues that videotex is basically an alpha-numeric information service and only limited block graphics are required to enhance the text. The other faction argues that good graphics are an essential part of a videotex information service and are required to effectively communicate the information. Because of the wide range of information to be provided by videotex services it is likely that both faCtions are correct and in fact a wide range of terminals will be required. In the opinion of the authors of this paper, the graphics coding scheme used for videotex services should be capable of accommodating these variable terminal requirements and permit a maximum cf independence between the terminal, the communications medium used, and the data bases. In other words, a single data base should be capable of serving terminals with a variety of graphics qualities over a variety of communications mediums without any need for special processing for any particular terminal.
Pulse transmission through a saturable absorber (1967)

A differential equation is derived and used to calculate the dynamic response of a saturable absorber to an incident light pulse of high intensity and short duration. Particular reference is made to the use of the phthalocyanine dyes which have been employed for laser Q-switching. It is found that high-intensity pulses are transmitted with little distortion or attenuation, but those of intermediate intensity show some reduction in pulse width and a delay in reaching peak intensity, in addition to suffering some attenuation. For a ruby-laser-phthalocyanine-dye system these effects would be expected for nanosecond pulse widths and peak intensities in the region of 106 w cm-2. The application of the theory in the design of a laser amplifier chain for pulse sharpening is indicated briefly.
Antiviral Activity of Distamycin A against Vaccinia Virus Is the Result of Inhibition of Postreplicative mRNA Synthesis (2004)

Distamycin A has been described as an inhibitor of the cellular pathogenesis of vaccinia virus in culture. Distamycin is an antibiotic that specifically targets the minor groove of DNA. We show here that distamycin is a potent inhibitor of vaccinia virus replication. Pulse-labeling experiments showed that most major late proteins failed to accumulate in the presence of the antibiotic. We characterized the effect of distamycin on vaccinia virus nucleic acid biosynthesis with the goal of determining the inhibitor's target. Early gene transcription was unaffected. DNA synthesis proceeded at normal rates, but DNA accumulated in large masses in the cytoplasm with no evidence of virion assembly. Transcription from the intermediate class promoter for the I1L gene was partially reduced by distamycin; however, transcription from the intermediate promoters for the three late transcription factor genes was severely inhibited. The accumulation of the late transcripts for the viral F17R and A10L genes also was severely impaired and was shown to be a direct inhibition of late promoter activity. These results indicate that inhibition of postreplicative intermediate and late transcription is the basis for inhibition of vaccinia virus by distamycin and indicate that DNA minor-groove ligands hold promise for effective anti-poxvirus drugs.
High-Performance Liquid Chromatography With Porous Silica Microspheres (1972)

Journal Article High-Performance Liquid Chromatography With Porous Silica Microspheres Get access J. J. Kirkland J. J. Kirkland Biochemicals Department, Experimental Station, E. I. DuPont de Nemours and Company, Wilmington, Delaware 19898 Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Chromatographic Science, Volume 10, Issue 10, October 1972, Pages 593–599, https://doi.org/10.1093/chromsci/10.10.593 Published: 01 October 1972 Article history Received: 01 July 1972 Published: 01 October 1972
Electrogenerated electrophilic reagents from sulphur: a new access to bis-methoxyaryl sulphides (1990)

The oxidation of a sulphur/carbon anode may produce electrophilic species derived from sulphur, and addition of aromatic ethers to the solution allows the two-stage formation of the corresponding diaryl sulphides.
High Performance Electron Cyclotron Resonance Plasma Etching with Control of Magnetic Field Gradient (1991)

It is shown that high-performance Electron Cyclotron Resonance (ECR) plasma (hyper-ECR) is effective as a dry etching technique for fabrication of VLSI devices. The gradient of the magnetic field is one of the most important parameters for a uniform high etch rate. The Si etch rate and the ion current density can rise with decreasing magnetic field gradient. The high uniformity of the etch rate is achieved with highly uniform distribution of the magnetic field gradient. The density distribution of plasma is also improved by the hyper-ECR, so the drift instability is stabilized and a no-microloading etching process is produced.
Cellular apoptosis is associated with increased caveolin-1 expression in macrophages (2003)

Macrophage apoptosis is an important factor in determining the efficiency of the immune response, atherosclerotic lesion stability, and clearance of aged cells by phagocytosis. The involvement of caveolin-1 in the regulation of apoptosis has been previously suggested in fibroblasts and epithelial cells. Here we show that treatment of thioglycollate-elicited mouse peritoneal macrophages with various unrelated apoptotic agents, including simvastatin, camptothecin, or glucose deprivation, is associated with a specific and large increase in caveolin-1 expression. In contrast, caveolin-2 levels remain unaffected. Induction of apoptosis was measured by changes in cell morphology, annexin V-labeling, and DNA fragmentation. We demonstrate that caveolin-1 in macrophages is present in lipid rafts and colocalizes with phosphatidylserine (PS) at the cell surface of apoptotic macrophages. Our data suggest that caveolin-1 increase is an early event, closely accompanied by PS externalization and independent of caspase activation and nuclear DNA fragmentation. The increase in caveolin-1 levels does not require new protein synthesis, as cycloheximide does not prevent the apoptosis-mediated increase in caveolin-1 levels. We propose that increased levels of caveolin-1 characterize the apoptotic phenotype of macrophages. Caveolin-1 may be involved in the efficient externalization of PS at the surface of the apoptotic cells.
Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA. (2003)

siRNA may be effective in inhibiting SARS-associated coronavirus replication.
Electric Field Induced Magnetic Anisotropy in a Ferromagnet (2009)

We report the first observation of a transient all electric field induced magnetic anisotropy in a thin film metallic ferromagnet. We generate the anisotropy with a strong (approximately 10(9) V/m) and short (70 fs) E-->-field pulse. This field is large enough to distort the valence charge distribution in the metal, yet its duration is too brief to change the atomic positions. This pure electronic structure alteration of the sample generates a new type of transient anisotropy axis and strongly influences the magnetization dynamics. The successful creation of such an anisotropy opens the possibility for all E-->-field induced magnetization reversal in thin metallic films--a greatly desired yet unachieved process.
Mutation analysis and characterization of COL7A1 mutations in dystrophic epidermolysis bullosa (2008)

Dystrophic epidermolysis bullosa (DEB) is inherited in both an autosomal dominant DEB and autosomal recessive manner RDEB, both of which result from mutations in the type VII collagen gene (COL7A1). To date, 324 pathogenic mutations have been detected within COL7A1 in different variants of DEB; many mutations are clustered in exon 73 (10.74%) which is close to the 39 amino acid interruption region. Dominant dystrophic epidermolysis bullosa usually involves glycine substitutions within the triple helix of COL7A1 although other missense mutations, deletions or splice-site mutations may underlie some cases. In recessive dystrophic epidermolysis bullosa, the mutations include nonsense, splice site, deletions or insertions, 'silent' glycine substitutions within the triple helix and non-glycine missense mutations within the triple helix or non-collagenous NC-2 domain. The nature of mutations in COL7A1 and their positions correlate reasonably logically with the severity of the resulting phenotypes.
Output Feedback Fuzzy H ∞ Control of Nonlinear Systems withTime-Varying Delayed State (2000)

Output Feedback Fuzzy H∞ Control of Nonlinear Systems with Time-Varying Delayed State Kap Rai Lee Abstract: This paper presents an output feedback fuzzyH∞ control problem for a class of nonlinear systems with time-varying delayed state. The Takagi-Sugeno fuzzy model is employed to represent a nonlinear systems with time-varying delayed state. Using a single quadratic Lyapunov function, the globally exponential stability and disturance attenuation of the closed-loop fuzzy control system are discussed. Suf cient conditions for the existence of fuzzy H∞ controllers are given in terms of matrix inequalities. Constructive algorithm for design of fuzzy H∞ controller is also developed. A simulation example is given to illustrate the performance of the proposed design method.
Horting hatches an egg (1999)

Article Free Access Share on Horting hatches an egg: a new graph-theoretic approach to collaborative filtering Authors: Charu C. Aggarwal IBM T. J. Watson Research Center, Yorktown Heights, NY IBM T. J. Watson Research Center, Yorktown Heights, NYView Profile , Joel L. Wolf IBM T. J. Watson Research Center, Yorktown Heights, NY IBM T. J. Watson Research Center, Yorktown Heights, NYView Profile , Kun-Lung Wu IBM T. J. Watson Research Center, Yorktown Heights, NY IBM T. J. Watson Research Center, Yorktown Heights, NYView Profile , Philip S. Yu IBM T. J. Watson Research Center, Yorktown Heights, NY IBM T. J. Watson Research Center, Yorktown Heights, NYView Profile Authors Info & Claims KDD '99: Proceedings of the fifth ACM SIGKDD international conference on Knowledge discovery and data miningAugust 1999 Pages 201–212https://doi.org/10.1145/312129.312230Published:01 August 1999Publication History 218citation2,375DownloadsMetricsTotal Citations218Total Downloads2,375Last 12 Months144Last 6 weeks44 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Particle Filters for Robot Navigation (2012)

Autonomous navigation is an essential capability for mobile robots. In order to operate robustly, a robot needs to know what the environment looks like, where it is in its environment, and how to navigate in it. This works summarizes approaches that address these three problems and that use particle filters as their main underlying model for representing beliefs. We illustrate that these filters are powerful tools that can robustly estimate the state of the robot and its environment and that it is also well-suited to make decisions about how to navigate in order to minimize its uncertainty of the joint belief about its own position and the state of the environment.
Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production (2017)

Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
Intravascular laser therapy of acute myocardial infarction. (1990)

The efficacy of a new method of treatment, endovascular blood irradiation with He-Ne laser, developed by the authors, was studied in 295 patients with primary acute transmural myocardial infarction (MI). Twenty-four-hour Holter monitoring findings before and after laser irradiation suggested the possibility of preventing sudden death in the acute period of MI owing to the high anti-arrhythmic efficacy of the method with respect to high-grade ventricular arrhythmias. Precodial ECG mapping and serial determination of blood enzyme activities (CPK and MB-CPK) showed that irradiation performed within the first hours of MI development contributed to effective limitation of the infarction area and restricted the spread of the area of myocardial ischemic damage.
<title>Thermo-optic switch and wavelength tunable filter using polymer waveguides</title> (2001)

We have developed a 2 by 2 digital optical switch (DOS) composed of four 1 by 2 DOS elements and a thermo-optic wavelength tunable filter using silicone resin waveguides. The 2 by 2 DOS operated with a switching power of 380 mW, a very low crosstalk of less than -60 dB, and a low insertion loss of less than 2.5 dB at 1.55 micrometers . The tunable filter operated with a maximum power of 2.5 W, a tuning range of &gt; 30 nm, a crosstalk of &lt; -30 dB, and response time of &lt; 60 ms. We also applied our polymer tunable filter to a WDM/SCM LAN system to confirm its practicality.
Toxicity and anticancer activity of a new triazine antifolate (NSC 127755). (1982)

A new triazine folate antagonist, 3-chloro-4-((4-(2-chloro-4-[4,6-diamino-2,2-dimethyl-s-triazin-1(2H)-yl]phenyl)butyl)) benzenesulfonyl fluoride compounded with ethanesulfonic acid (1:1) (NSC 127755), was highly active against four transplantable colon adenocarcinomas (36, 38, 10/A, 12/A) and the Dunning murine ovarian tumor M5076. Treatment schedule studies indicated that a prolonged time of exposure provided optimum antitumor activity for the compound. The combination of NSC 127755 plus 4-amino-1-[5-O-(1-oxohexadecyl)-beta-D-arabinofuranosyl]-2(1H)-pyrimidinone (palmO-ara-C, NSC 135962) was found to have therapeutic synergism against grossly evident colon adenocarcinoma 36.