gebhart/patrepeval huggingface dataset:
Multiple-peptidase mutants of Lactococcus lactis are severely impaired in their ability to grow in milk (1996)

To examine the contribution of peptidases to the growth of lactococcus lactis in milk, 16 single- and multiple-deletion mutants were constructed. In successive rounds of chromosomal gene replacement mutagenesis, up to all five of the following peptidase genes were inactivated (fivefold mutant): pepX, pepO, pepT, pepC, and pepN. Multiple mutations led to slower growth rates in milk, the general trend being that growth rates decreased when more peptidases were inactivated. The fivefold mutant grew more than 10 times more slowly in milk than the wild-type strain. In one of the fourfold mutants and in the fivefold mutant, the intracellular pools of amino acids were lower than those of the wild type, whereas peptides had accumulated inside the cell. No significant differences in the activities of the cell envelope-associated proteinase and of the oligopeptide transport system were observed. Also, the expression of the peptidases still present in the various mutants was not detectably affected. Thus, the lower growth rates can directly be attributed to the inability of the mutants to degrade casein-derived peptides. These results supply the first direct evidence for the functioning of lactococcal peptidases in the degradation of milk proteins. Furthermore, the study provides critical information about the relative importance of the peptidases for growth in milk, the order of events in the proteolytic pathway, and the regulation of its individual components.
Can collagen metabolism be controlled? (1984)

The rate of repair of severely burned tissue may be limited by the availability of both non-damaged connective tissue cells and factors which control their movement and proliferation. Supplementation of wounds with chemotactic and growth factors contained in a matrix support may be useful in accelerating the healing of these wounds. Addition of dermal fibroblasts to this matrix may also be beneficial in preventing the formation of contracted scars. Future studies directed at identifying and isolating these factors and testing their efficiency in in vivo wound systems should prove useful in developing new approaches to severe wound care and for individuals who suffer from poor wound healing such as diabetics or aged people.
Amphiphilic Polymethacrylate Derivatives as Antimicrobial Agents (2005)

We have investigated the structure-activity relationship of cationic amphiphilic polymethacrylate derivatives in antimicrobial and hemolytic assays. The polymers were prepared by radical copolymerizations of N-(tert-butoxycarbonyl)aminoethyl methacrylate and butyl methacrylate in the presence of methyl 3-mercaptopropionate as a chain transfer agent to give precursor polymers protected with a tert-butoxycarbonyl (Boc) group. Subsequent treatment of the Boc-protected polymers with TFA affords the desired cationic random copolymers. We examined antimicrobial and hemolytic activities of a series of polymers having a wide range of mole percentage of butyl groups (0-60%) in three different molecular weight (MW) ranges. The smallest polymers (MW < 2000) showed the lowest MIC and reduced hemolytic activity compared to that of the higher MW ones. In addition, polymers containing a high percentage of butyl groups are less selective for bacterial cells than their less hydrophobic counterparts.
CcpB, a Novel Transcription Factor Implicated in Catabolite Repression in Bacillus subtilis (1998)

Recent work has shown that in Bacillus subtilis catabolite repression of several operons is mediated by a mechanism dependent on DNA-binding protein CcpA complexed to a seryl-phosphorylated derivative of HPr [HPr(Ser-P)], the small phosphocarrier protein of the phosphoenolpyruvate-sugar phosphotransferase system. In this study, it was found that a transposon insertional mutation resulted in the partial loss of gluconate (gnt) and xylose (xyl) operon catabolite repression by glucose, mannitol, and sucrose. The transposon insertion was localized to a gene, designated ccpB, encoding a protein 30% identical to CcpA, and relief from catabolite repression was shown to be due to the absence of CcpB rather than to the absence of a protein encoded by a downstream gene within the same operon. The relative intensities of CcpA- and CcpB-mediated catabolite repression depended on growth conditions. On solid media, and when cells were grown in liquid media with little agitation, CcpB and CcpA both proved to function in catabolite repression. However, when cells were grown in liquid media with much agitation, CcpA alone mediated catabolite repression. Like CcpA, CcpB appears to exert its catabolite-repressing effect by a mechanism dependent on the presence of HPr(Ser-P).
Lidocaine Adrenaline Tetracaine Gel Versus Tetracaine Adrenaline Cocaine Gel for Topical Anesthesia in Linear Scalp and Facial Lacerations in Children Aged 5 to 17 Years (1995)

LAT gel worked as well as TAC gel for topical anesthesia in facial and scalp lacerations. Considering the advantages of a noncontrolled substance and less expense, LAT gel appears to be better suited than TAC gel for topical anesthesia in laceration repair in children.
Transient and restricted expression during mouse embryogenesis of Dll1, a murine gene closely related to Drosophila Delta (1995)

The Drosophila Delta (Dl) gene is essential for cell-cell communication regulating the determination of various cell fates during development. Dl encodes a transmembrane protein, which contains tandem arrays of epidermal-growth-factor-like repeats in the extracellular domain and directly interacts with Notch, another transmembrane protein with similar structural features, in a ligand-receptor-like manner. Similarly, cell-cell interactions involving Delta-like and Notch-like proteins are required for cell fate determinations in C. elegans. Notch homologues were also isolated from several vertebrate species, suggesting that cell-to-cell signaling mediated by Delta- and Notch-like proteins could also underlie cell fate determination during vertebrate development. However, in vertebrates, no Delta homologues have yet been described. We have isolated a novel mouse gene, Dll1 (delta-like gene 1), which maps to the mouse t-complex and whose deduced amino acid sequence strongly suggests that Dll1 represents a mammalian gene closely related to Drosophila Delta. Dll1 is transiently expressed during gastrulation and early organogenesis, and in a tissue-restricted manner in adult animals. Between day 7 and 12.5 of development, expression was detected in the paraxial mesoderm, closely correlated with somitogenesis, and in subsets of cells in the nervous system. In adult animals, transcripts were detected in lung and heart. Dll1 expression in the paraxial mesoderm and nervous system is strikingly similar to the expression of mouse Notch1 during gastrulation and early organogenesis. The overlapping expression patterns of the Dll1 and Notch1 genes suggest that cells in these tissues can communicate by interaction of the Dll1 and Notch1 proteins. Our results support the idea that Delta- and Notch-like proteins are involved in cell-to-cell communication in mammalian embryos and suggest a role for these proteins in cellular interactions underlying somitogenesis and development of the nervous system.
Vasovasostomy in the murine vas deferens: Comparison of the Nd:YAG laser at 1.06 microns and 1.318 microns to the CO2 laser (1988)

A comparison is made of laser anastomoses of the murine vas deferens at different energies with the neodymium (Nd):YAG laser at 1.06 micron and 1.318 micron and with the CO2 laser. A total of 28 welds were performed with a free-hand technique employing a 600-micron silicon fiber with the Nd:YAG and a hand piece with a 500-micron spot size for the CO2. After 6 weeks, all animals were sacrificed and the vasa evaluated for patency. Fifteen out of 28 controls repaired with microsurgical techniques were found to be patent; 4/10 vasa were patent with use of the Nd:YAG at 1.318 micron at laser energies of 300 mW and 500 mW. At 1.06 micron, only 1/4 anastomoses was patent at a power setting of 1 W. None of the anastomoses performed with the CO2 laser was patent. Histologic study revealed intense fibrosis in all the lasered vasa, with sperm granuloma formation associated with most anastomoses. Although this is a preliminary study, it appears that the Nd:YAG laser at 1.318 micron and a power setting of 300-500 mW provides patency rates superior to the Nd:YAG at 1.06 micron and to the CO2 lasers and is equivalent to standard micro-surgical techniques in the murine vas deferens.
Challenges and opportunities for integrated optics in optical networks (1999)

Ever-increasing needs for more telecommunication bandwidth have brought about the recent emergence of WDM technology as a commercial reality with a very substantial volume, and prospects for near future development of optical networking. The new optical functionalities in networks will also provide conmiercial opportunities for integrated optics, which have been yearned for a long time. Integrated optics must meet the new challenges of fulfilling the strict performance, reliability and cost requirements for applications in the field. Integrated optics community is experiencing a transition to a new era, with their work moving from research laboratories to industrial production. Various expected applications for integrated optics devices in the areas of wavelength division multiplexing, optical modulation, optical switching, wavelength conversion and others are reviewed. General views on development of packaging technologies, component design and testing are also included.
An Effective Algorithm for Extracting Serial Endocardial Borders from 2-Dimensional Echocardiograms (1984)

An effective image processing algorithm for extracting the endocardial border from a series of 2-dimensional echocardiograms (2D echo) is proposed. The main steps involved in this algorithm are coordinate transformation, regional co-occurrence matrix thresholding, search region limiting, border detecting, and spatial and temporal smoothing. Using these steps, some major difficulties related to 2D echo image processing including low signal-to-noise ratio, echo drop out, and gray level intensity variability have been dramatically reduced. All endocardial borders in an entire cardiac cycle are computed. This is done automatically on the basis of observer-drawn outlines at end systole and end diastole. These outlines facilitate the quantitative analysis by defining a range in which both boundary dimension and shape changes should occur. Image processing is restricted to this range. The validation studies have been perfonned on test balloons where both image quality has been changed and complex motion abnormalities have been experimentally produced. Good results have also been obtained in patient studies.
Molecular structure of the water channel through aquaporin CHIP. The hourglass model. (1994)

Aquaporin channel-forming integral protein (CHIP) is the first characterized water channel protein (genome symbol AQP1), but the molecular structure of the aqueous pathway through CHIP remains undefined. The two halves of CHIP are sequence-related, and each has three bilayer-spanning domains with the motif asparagine-proline-alanine (NPA) at residues 76-78 (in cytoplasmic loop B) and 192-194 (in extracellular loop E). The NPA motifs are oriented 180 degrees to each other, and the second NPA is near cysteine 189, the known site where mercurials inhibit osmotic water permeability (Pf). When expressed in Xenopus oocytes, the double mutant A73C/C189S exhibited high, mercurial-sensitive Pf similar to wild-type CHIP. Conservative substitutions of slightly greater mass in or near NPA motifs in loop B or loop E in CHIP caused reduced Pf and failure of the protein to localize at the plasma membrane. Certain nonfunctional loop E mutants complemented the truncation mutant D237Z. Formation of mixed oligomers was demonstrated by velocity sedimentation, immunoprecipitation, and analysis of dimeric-CHIP polypeptides. Cellular distributions of individual mutants or complementing pairs of mutants were verified by plasma membrane isolation and confocal microscopy. An hourglass structural model is proposed in which a cytoplasmic chamber (loop B) connects within the membrane to an extracellular chamber (loop E) forming a single, narrow aqueous pathway through each of the CHIP subunits; subunit oligomerization may provide the vertical symmetry necessary for residence within the lipid bilayer.
Succinic acid production with reduced by‐product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source (2001)

Succinic acid was produced by fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. When cells were anaerobically cultured in a medium containing 6.5 g/L glycerol, a high succinic acid yield (133%) was obtained while avoiding the formation of by-product acetic acid. The gram ratio of succinic acid to acetic acid was 25.8:1, which is 6.5 times higher than that obtained using glucose (ca. 4:1) as a carbon source. Therefore, succinic acid can be produced with much less by-product formation by using glycerol as a carbon source, which will facilitate its purification. When glucose and glycerol were cofermented with the increasing ratio of glucose to glycerol, the gram ratio of succinic acid to acetic acid and succinic acid yield decreased, suggesting that glucose enhanced acetic acid formation irrespective of the presence of glycerol. Glycerol consumption by A. succiniciproducens required unidentified nutritional components present in yeast extract. By intermittently feeding yeast extract along with glycerol, a high succinic acid yield (160%) could be obtained while still avoiding acetic acid formation. This resulted in the highest ratio of succinic acid to acetic acid (31.7:1). © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 41–48, 2001.
JAK2 V617F Mutational Status in Myelofibrosis at and before Disease Progression Including Leukemic Transformation: A Longitudinal Study in 68 Patients. (2005)

Abstract Background: An acquired point mutations of JAK2 (V617F) was recently described in a substantial proportion of patients with myeloproliferative disorders (MPD), including myelofibrosis with myeloid metaplasia (MMM). The current study examines i) JAK2 V617F mutational status at time of leukemic transformation (LT) of MMM and its clinical correlates, ii) the possibility of changes over time in mutational status in MMM patients that have either undergone leukemic transformation (LT) or experienced disease progression. Methods: Mutation analysis for JAK2 V617F was performed in DNA derived from either peripheral blood mononuclear cells (PBMC) (i.e. fresh specimens) using genomic DNA amplified by PCR, or extracted from cytogenetic pellets in archived specimens. Fluorescent dye chemistry sequencing was performed using the same primers used for amplification. Results: The study population consisted of 68 patients including 33 patients with disease transformation into AML. i. MMM patient cohort with LT (n=33). The JAK2 V617F mutant allele was detected in 21 of the 33 patients (64%; homozygous in 6%) at the time of LT. As expected, PPMM was over-represented in the group with the mutation but the presence of the mutation affected neither the clinical presentation at time of LT or overall outcome. Furthermore, in 9 patients (7 heterozygotes and 2 with wild-type allele), archived bone marrow that was collected at a median of 23 months (range, 3–105) before LT was available and disclosed no change in mutational status. ii. MMM patient cohort with disease progression without LT (n=35; 19 AMM, 9 PPMM, 7 PTMM): Each patient in this group had at least two bone marrow or peripheral blood samples that were collected at two different time points separated by a median of 18.2 months (range (3–82). Of the total 35 patients in this group, 32 (91%) showed no change in JAK2 V617F mutational status over time including 3 patients in whom the first sample was collected before they transformed into MMM from antecedent PV or ET. In the remaining 3 patients, a switch from wild-type to heterozygous state (1 patient), and heterozygous to homozygous (2 patients), was documented after 78, 43, and 83 months, respectively. Conclusions: In most instances, JAK2 V617F mutational status in MMM remains unchanged over time including during leukemic transformation. Furthermore, the presence of the mutation in MMM-related LT does not appear to affect clinical outcome. These observations undermine the role of JAK2 V617F in either disease progression or LT in MMM.
Triaging dysphagia: nurse screening for dysphagia in an acute hospital (2009)

Twenty-five to 30% of acute hospitalised individuals have dysphagia. All adult acute patients are screened for dysphagia using the Royal Brisbane and Women's Hospital dysphagia screening tool. Patients are triaged into categories of 'those requiring additional specialist intervention' and 'those who can proceed directly to regular diets and liquids'. Improved quality of care and cost savings is likely.
Probiotic Bacteria as Biological Control Agents in Aquaculture (2000)

There is an urgent need in aquaculture to develop microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and trade and since the development of antibiotic resistance has become a matter of growing concern. One of the alternatives to antimicrobials in disease control could be the use of probiotic bacteria as microbial control agents. This review describes the state of the art of probiotic research in the culture of fish, crustaceans, mollusks, and live food, with an evaluation of the results obtained so far. A new definition of probiotics, also applicable to aquatic environments, is proposed, and a detailed description is given of their possible modes of action, i.e., production of compounds that are inhibitory toward pathogens, competition with harmful microorganisms for nutrients and energy, competition with deleterious species for adhesion sites, enhancement of the immune response of the animal, improvement of water quality, and interaction with phytoplankton. A rationale is proposed for the multistep and multidisciplinary process required for the development of effective and safe probiotics for commercial application in aquaculture. Finally, directions for further research are discussed.
Integrated Analysis of Multiple Microarray Datasets Identifies a Reproducible Survival Predictor in Ovarian Cancer (2011)

Integration of previously generated cancer microarray datasets may lead to robust and widely applicable survival predictors. These predictors are not simply a compilation of prognostic genes but appear to track true molecular phenotypes of good- and poor-outcome.
A Peer Counselling Service in a Secondary School to Combat Bullying: Issues in Planning and Ongoing Management (2005)

Peer counselling schemes have been developed in some secondary schools to combat bullying. Research supports the need for bullied victims to speak with their peers. Such schemes need regular monitoring and modification to suit the school's particular pastoral needs. This paper explores the management issues surrounding the provision of peer counselling in one service, managed by a school counsellor. The service is outlined to show that appointment setting requires careful planning and ongoing management. This paper discusses four central issues, which are the role of a peer counselling service; the selected model; the most suitable room location; and the most appropriate time for counselling to take place in secondary school.
Four channel fibre grating demultiplexer (1994)

The authors demonstrate a four channel all-fibre demultiplexer based on a 1×4 fused fibre coupler followed by strong fibre grating passband filters.
Human T lymphocyte activation by monoclonal antibodies; OKT3, but not UCHT1, triggers mitogenesis via an interleukin 2-dependent mechanism. (1984)

OKT3 and UCHT1 monoclonal antibodies, which recognize the same human T cell surface antigen, induce proliferation in T lymphocytes. In this report, we compared the mechanism by which these antibodies trigger DNA synthesis in human peripheral blood mononuclear cell (PBMC) cultures. Whereas PBMC from all donors tested were mitogenically inducible by OKT3, cells from only 25 of 40 donors were responsive to UCHT1 . UCHT1 treatment of PBMC from responders, but not from nonresponders, resulted in the expression by T cells of membrane binding sites reactive with anti-Tac monoclonal antibody, which specifies the human interleukin 2 (IL 2) receptor. UCHT1 -induced PBMC supernatants from nonresponders, but unexpectedly, also from responders, contained no measurable IL 2 activity. In keeping with this finding, anti-Tac monoclonal antibody failed to suppress UCHT1 -triggered [3H]thymidine incorporation into PBMC from responsive donors. By contrast, OKT3 treatment of PBMC from all donors led to the emergence of IL 2 receptors, and substantial IL 2 production, and the resultant DNA synthesis was inhibitable by anti-Tac antibody. These data indicate that the interaction of OKT3 and UCHT1 monoclonal antibodies with the same T cell structure leads to the induction of proliferation via two different mechanisms: one dependent on the availability of IL 2 (OKT3) and one independent on the production and processing of this lymphokine ( UCHT1 ). PBMC unresponsiveness to UCHT1 could therefore not be related to a dysfunction in IL 2 synthesis or IL 2 receptor display.
Ueber die N‐Methylolverbindungen der Säureamide [Erste Abhandlung.] (1905)

einiger Zeit bekannt, dass einzelne Saureimide die
Object fitting to the bispectral phase by using least squares (1993)

We present a new approach for the reconstruction of two-dimensional images directly from the bispectral phase. Unlike earlier methods, the system requires no information regarding the Fourier modulus, and thus the reference star measurement is unnecessary. We give examples that use experimental binary star data and simulated data of quadruple stars.
Memory, Flexibility, and Professional Risk (2003)

This article focuses on the role of organizational memory in bringing about professional 
risks and industrial accidents. The modelling 
of the concept of organizational memory by 
Walsh and Ugson ( 1991) sets out the necessity for the employee to have a steady working context made up of beliefs and 
knowledge shared through his social links in 
order to create his own personality. And yet 
the demand for current economic competitiveness is an incentive for organisations to 
resort to flexibility in various forms resulting 
(among other things) in the increase in the 
lack of job security. They do not enable their 
members, affected by its environmental and 
structural changes, to integrate that organizational memory, which thus brings about an 
increase in professional risks and industrial 
accidents for that category of individuals.
Improving New Mexico's Water Management (2003)

This paper reviews several measures that New Mexico should pursue to improve its management of water. The crisis in New Mexico’s water affects all of the citizens of the state, but hasty responses may promise more than they can deliver. In this paper we present several measures that will allow better use of the resources that the state has, and rectify imbalances in how water has been managed in the state.