gebhart/patrepeval huggingface dataset:
Pharmacokinetics and metabolism of [14C]-proglumetacin after oral administration in the rat. (1987)

The pharmacokinetics and metabolism of 1H-indole-3-acetic acid, 1-(4-chlorobenzoyl)-5-methoxy-2-methyl 2-[4-[3-[[4-(benzoylamino)-5-(dipropylamino)-1, 5-dioxopentyl]oxy]propyl]-1-piperazinyl]ethyl ester (+/-) (proglumetacin, CR 604), 14C-labelled in position C-2 of the indolic moiety, was studied in male and female rats after oral administration. The radioactivity is slowly absorbed from the gastrointestinal tract and reaches the peak in plasma 6 h after administration. Afterwards the radioactivity is eliminated from plasma according to a bi-exponential equation, with an initial elimination rate having a t1/2 of 4.5 h and a terminal elimination rate having a t1/2 of 16 h. The elimination rate is probably dependent on the slow absorption rate (flip-flop system) and on an entero-hepatic recirculation of the radioactivity. The radioactivity is excreted with the feces (60.8%) and with the urines (33.5%). No radioactivity is eliminated with the expired air. About 13% of the fecal radioactivity is represented by proglumetacin. This fraction or radioactivity (ca. 8% of the administered) represents probably the non-absorbed amount of substance. The parent proglumetacin is not found in blood, urine and organs. Conversely several indolic metabolites are found, with a predomination of indometacin. Proglumetacin is therefore a pro-drug of indometacin and of other indolic metabolites, which are responsible for the antiinflammatory activity of proglumetacin. The radioactivity is distributed in all tissues. The maximum radioactivity is found in liver and kidneys, however, always in a smaller concentration than in plasma. No "deep compartment" was found.
Biodiesel: Source, Production, Composition, Properties and Its Benefits (2006)

Biodiesel is an eco-friendly, alternative diesel fuel prepared from domestic renewable resources i.e. vegetable oils (edible or non-edible oil) and animal fats, that runs in diesel engines-cars, buses, trucks, construction equipment, boats, generators, and oil home heating units. Biodiesel has been gaining worldwide popularity as an alternative energy source because it is non toxic, biodegradable & non flammable. Various edible and non edible oils, like rice bran oil, coconut oil, Jatropha curcas, castor oil, cottonseed oil, mahua, karanja which are either surplus and are nonedible type can be used for preparation of biodiesel. Biodiesel can be used either in the pure form or as blends on conventional petrodiesel in automobiles without any major modifications. Its biodegradability makes it eco-friendly. It may lead to a revolutionary transformation of the current economic & energy scenario with an era of economic bloom & prosperity for our society. This review paper describes the production, its properties, composition and future potential of biodiesel.
Electrical Properties of Silicon Containing Arsenic and Boron (1954)

Electrical conductivity and Hall effect have been measured from 10\ifmmode^\circ\else\textdegree\fi{} to 1100\ifmmode^\circ\else\textdegree\fi{} Kelvin on single-crystal silicon containing arsenic and boron. Extrinsic carrier concentration is computed from Hall coefficient. Analysis of extrinsic carrier concentration indicates the ionization energy of arsenic donor levels to be 0.049 ev and of boron acceptor levels to be 0.045 ev for low impurity concentrations. Fermi degeneracy is found to occur in the range ${10}^{18}$ to ${10}^{19}$ ${\mathrm{cm}}^{\ensuremath{-}3}$ impurity concentration. Extrinsic Hall mobility is computed from Hall coefficient and conductivity. Curves of Hall mobility against resistivity at 300\ifmmode^\circ\else\textdegree\fi{}K are computed from theory and compared with experiment. The temperature dependence of lattice-scattering mobility is found from conductivity to be ${T}^{\ensuremath{-}2.6}$ for electrons and ${T}^{\ensuremath{-}2.3}$ for holes. From conductivity mobility and intrinsic conductivity, it is found that carrier concentration at any temperature below 700\ifmmode^\circ\else\textdegree\fi{}K is given by the expression: $np=1.5\ifmmode\times\else\texttimes\fi{}{10}^{33}{T}^{3}\mathrm{exp}(\ensuremath{-}\frac{1.21}{\mathrm{kT}})$. The temperature dependence of the ratio Hall mobility/conductivity mobility is determined for holes and electrons.
Studies on Synthesis and Properties of Surfactants with Specific Functions (1994)

Novel surfactants with specific functions were sinthesized from inexpensive, commercially available bulk chemicals by convenient synthetic methods. All were characterized by features such as chemical degradability, catalitic activity for a halide displacement reaction (Finkelstein reaction), ability to disperse lime soap and complex with alkali metal cations. Applications for emulsion polimerization, surface-active properties, stability constants of complexes with alkali metal ions, and solubilization of alkali metal picrates in organic solvents were studies.
Function of arginase in lactating mammary gland (1972)

The potential for a considerable formation of ornithine exists in lactating mammary gland because of its arginase content. Late in lactation arginase reaches an activity in the gland higher than that present in any rat tissue except liver. Occurrence of the urea cycle can be excluded since two enzymes for the further reaction of ornithine in the cycle, carbamoyl phosphate synthetase I and ornithine carbamoyltransferase, are both absent from this tissue. Instead, carbamoyl phosphate synthetase II appears early in lactation, associated with accumulation of aspartate carbamoyltransferase and DNA, consistent with the proposed role of these enzymes in pyrimidine synthesis. The facts require another physiological role for arginase apart from its known function in the urea cycle. Significant activity of ornithine aminotransferase develops in mammary gland in close parallel with the arginase. By this reaction, ornithine can be converted into glutamic semialdehyde and subsequently into proline. The enzymic composition of the lactating mammary gland is therefore appropriate for the major conversion of arginine into proline that is known to occur in the intact gland.
Why do they die? Comparison of selected aspects of organ injury and dysfunction in mice surviving and dying in acute abdominal sepsis (2015)

In CLP-Only mice, although the deregulation of parameters indicative of organ injury/dysfunction was greater in dying versus surviving mice, it never exceeded the changes in surviving CLP-ODam animals, and it was not followed by histopathological damage and/or mitochondrial dysfunction. This shows that interpretation of the contribution of the organ injury/dysfunction to early deaths in the CLP model is not straightforward and depends on the pathophysiological origin of the profiled disturbances.
Kinetic Mechanism for DNA Unwinding by Multiple Molecules of Dda Helicase Aligned on DNA (2010)

Helicases catalyze the separation of double-stranded nucleic acids to form single-stranded intermediates. Using transient state kinetic methods, we have determined the kinetic properties of DNA unwinding under conditions that favor a monomeric form of the Dda helicase as well as conditions that allow multiple molecules to function on the same substrate. Multiple helicase molecules can align like a train on the DNA track. The number of base pairs unwound in a single binding event for Dda is increased from approximately 19 bp for the monomeric form to approximately 64 bp when as many as four Dda molecules are aligned on the same substrate, while the kinetic step size (3.2 +/- 0.7 bp) and unwinding rate (242 +/- 25 bp/s) appear to be independent of the number of Dda molecules present on a given substrate. The data support a model in which the helicase molecules bound to the same substrate move along the DNA track independently during DNA unwinding. The observed increase in processivity arises from the increased probability that at least one of the helicases will completely unwind the DNA prior to dissociation. These results are in contrast to previous reports in which multiple Dda molecules on the same track greatly enhanced the rate and amplitude for displacement of protein blocks on the track. Therefore, only when the progress of the lead molecule in the train is impeded by some type of block, such as a protein bound to DNA, do the trailing molecules interact with the lead molecule to overcome the block. The fact that trailing helicase molecules have little impact on the lead molecule in the train during routine DNA unwinding suggests that the trailing molecules are moving at rates similar to that of the lead molecule. This result implicates a step in the translocation mechanism as contributing greatly to the overall rate-limiting step for unwinding of duplex DNA.
Complex Target-Oriented Synthesis in the Drug Discovery Process:  A Case History in the dEpoB Series (1999)

ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTComplex Target-Oriented Synthesis in the Drug Discovery Process: A Case History in the dEpoB Series and Samuel J. DanishefskyView Author Information Sloan-Kettering Institute for Cancer Research, Laboratory for Bioorganic Chemistry, 1275 York Avenue, New York, New York 10021, and the Department of Chemistry, Columbia University, Havemeyer Hall, New York, New York 10027 Cite this: J. Org. Chem. 1999, 64, 23, 8434–8456Publication Date (Web):October 23, 1999Publication History Received24 June 1999Published online23 October 1999Published inissue 1 November 1999https://pubs.acs.org/doi/10.1021/jo991006dhttps://doi.org/10.1021/jo991006dreview-articleACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views909Altmetric-Citations114LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aldehydes,Aldol reactions,Antineoplastic agents,Redox reactions,Rodent models Get e-Alerts
Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement (2018)

Influenza viruses replicate within the nucleus of the host cell. This uncommon RNA virus trait provides influenza with the advantage of access to the nuclear machinery during replication. However, it also increases the complexity of the intracellular trafficking that is required for the viral components to establish a productive infection. The segmentation of the influenza genome makes these additional trafficking requirements especially challenging, as each viral RNA (vRNA) gene segment must navigate the network of cellular membrane barriers during the processes of entry and assembly. To accomplish this goal, influenza A viruses (IAVs) utilize a combination of viral and cellular mechanisms to coordinate the transport of their proteins and the eight vRNA gene segments in and out of the cell. The aim of this review is to present the current mechanistic understanding for how IAVs facilitate cell entry, replication, virion assembly, and intercellular movement, in an effort to highlight some of the unanswered questions regarding the coordination of the IAV infection process.
Biodegradable polyphosphazenes for drug delivery (1997)

Abstract Polyphosphazene derivatives having amino acid ester side groups were prepared by reaction of poly(dichlorophosphazene) with ethyl esters of amino acids. The in vitro degradation studies demonstrated that the rate of degradation depends on the nature of the amino acids. Introducing small amounts of hydrolytically sensitive groups such as depsipeptide ester or hydrolysis‐catalysing moieties, such as histidine ethyl ester co‐substituents, resulted in an increase of the degradation. The rate of hydrolytic degradation of the polyphosphazene material could be controlled by the content of the hydrolytically sensitive side groups or by blending hydrolysis‐sensitive polymers with more stable derivatives. The results obtained from the in vivo implantation of biodegradable polyphosphazenes in mice indicate that the materials are very well tolerated by the animal body. Biodegradable polyphosphazenes have been used as matrix for the design of drug delivery systems. The rate of the in vitro release of mitomycin C from biodegradable polyphosphazenes can be controlled by changing the chemical composition of the polymer or by blending polymers of different chemical compositions.
Severe COVID-19: NLRP3 Inflammasome Dysregulated (2020)

SARS-CoV-2 might directly activate NLRP3 inflammasome resulting in an endogenous adjuvant activity necessary to mount a proper adaptive immune response against the virus. Heterogeneous response of COVID-19 patients could be attributed to differences in not being able to properly downregulate NLRP3 inflammasome activation. This relates to the fitness of the immune system of the individual challenged by the virus. Patients with a reduced immune fitness can demonstrate a dysregulated NLRP3 inflammasome activity resulting in severe COVID-19 with tissue damage and a cytokine storm. We sketch the outlines of five possible scenarios for COVID-19 in medical practice and provide potential treatment options targeting dysregulated endogenous adjuvant activity in severe COVID-19 patients.
An Experimental Vaccine Expressing Wild‐Type p53 Induces Protective Immunity against Glioblastoma Cells with High Levels of Endogenous p53 (2002)

Inoculation of mice with a recombinant vaccinia virus expressing the full-length mouse wild-type p53 protein (Vp53-wt) was shown to induce partial protection against peripheral challenge with a mouse glioblastoma cell line, termed GL261, expressing high levels of nuclear, endogenous wild-type p53. In vivo experiments with knockout (KO) mice and mice treated with depleting doses of antibodies specific to lymphocyte subsets revealed that vaccine efficacy depended on CD4+ and CD8+ T cells as well as on natural killer (NK) cells. Vp53-wt virus-vaccinated mice that failed to develop tumours upon challenge with a minimal tumourigenic dose of GL261 cells remained completely resistant to further challenge with increased doses of GL261 cells. The efficacy of the Vp53-wt vaccine was improved by adding recombinant mouse interleukin-12 (rIL-12) as an adjuvant at the time of tumour challenge. The induction of T cells to p53 in Vp53-wt virus-immune mice was also demonstrated at the tumour site by immunochemistry and was further confirmed by a delayed-type hypersensitivity response to the p53 protein, although in vitro experiments using splenocytes from vaccinated mice failed to demonstrate CD4+ or CD8+ T-cell activity to p53.
Cell-type and Donor-specific Transcriptional Responses to Interferon-α (2002)

A sensitive, specific, reproducible, robust, and cost-effective customized cDNA array system based on established nylon membrane technology has been developed for convenient multisample expression profiling for several hundred genes of choice. The genes represented are easily adjusted (depending on the availability of corresponding cDNAs) and the method is accordingly readily applicable to a wide variety of systems. Here we have focused on the expression profiles for interferon-alpha2a, the most widely used interferon for the treatment of viral hepatitis and malignancies, in primary cells (peripheral blood mononuclear cells, T cells, and dendritic cells) and cell lines (Kit255, HT1080, HepG2, and HuH7). Of 150 genes studied, only six were consistently induced in all cell types and donors, whereas 74 genes were induced in at least one cell type. IRF-7 was identified as the only gene exclusively induced in the hematopoietic cells. No gene was exclusively induced in the nonhematopoietic cell lines. In T cells 12, and in dendritic cells, 25 genes were induced in all donors whereas 45 and 42 genes, respectively, were induced in at least one donor. The data suggest that signaling through IFN-alpha2 can be substantially modulated to yield significant cell-type and donor-specific qualitative and quantitative differences in gene expression in response to this cytokine under highly standardized conditions.
Food of striped dolphin, Stenella coeruleoalba, in the Ligurian Sea (1993)

The stomachsof 23 striped dolphins ( Stenella coeruleoalba Meyen, 1833, Cetacea), stranded along the Ligurian coast (western Mediterranean Sea), contained 32 species of cephalopods, crustaceans and fishes, totalling an estimated 2,723 prey specimens representing about 36 kg in weight. Cephalopods and bony fishes were equally important in the diet (50%). Todarodes sagittatus (34.5%) and Micromesistius poutassou (25.9%) were found to be the most important food species. Other species belonging to six cephalopod families, three crustacean families and nine bony fish families, contributed to the diet with variable numbers, weights, and occurrences, demonstrating the opportunistic character of striped dolphin feeding.
Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress (2009)

Considerable interest has been aroused in recent years by the well-known notion that biological systems are sensitive to visible light. With clinical applications of visible radiation in the far-red to near-infrared region of the spectrum in mind, we explored the effect of coherent red light irradiation with extremely low energy transfer on a neural cell line derived from rat pheochromocytoma. We focused on the effect of pulsed light laser irradiation vis-à-vis two distinct biological effects: neurite elongation under NGF stimulus on laminin-collagen substrate and cell viability during oxidative stress. We used a 670 nm laser, with extremely low peak power output (3 mW/cm2) and at an extremely low dose (0.45 mJ/cm2). Neurite elongation was measured over three days in culture. The effect of coherent red light irradiation on cell reaction to oxidative stress was evaluated through live-recording of mitochondria membrane potential (MMP) using JC1 vital dye and laser-confocal microscopy, in the absence (photo bleaching) and in the presence (oxidative stress) of H2O2, and by means of the MTT cell viability assay. We found that laser irradiation stimulates NGF-induced neurite elongation on a laminin-collagen coated substrate and protects PC12 cells against oxidative stress. These data suggest that red light radiation protects the viability of cell culture in case of oxidative stress, as indicated by MMP measurement and MTT assay. It also stimulates neurite outgrowth, and this effect could also have positive implications for axonal protection.
System architecture and techniques for gesture recognition in unconstrained environments (2002)

Controlling appliances in home environments by gestures is a step towards more intuitive and natural human computer interfaces. A brief overview of an existing vision based gesture recognition system and its architecture and details of ergonomic remote control of devices by gestures are clarified. The focus is on motion detection, object normalization and identification, modelling, and prediction of motion using a Kalman filter. The initialization problem of the Kalman filter of a vision based system for human motion tracking differs from initialization for physical systems, where manuals report measurement errors. One main aim was to develop the initialization and adequate Kalman model for human motion. Most aspects mentioned in the report were implemented in the ARGUS prototype.
A Novel Method to Measure Self-association of Small Amphipathic Molecules (2003)

Biophysical techniques such as size-exclusion chromatography, sedimentation equilibrium analytical ultracentrifugation, and non-denaturing gel electrophoresis are the classical methods for determining the self-association of molecules into dimers, trimers, or other higher order species. However, these techniques usually require high (mg/ml) loading concentrations to detect self-association and also possess a lower size limit that is dependent on the ability of the technique to resolve monomeric from higher order species. Here we describe a novel, sensitive method with no upper or lower molecular size limits that indicates self-association of molecules driven together by the hydrophobic effect under aqueous conditions. "Temperature profiling in reversed-phase chromatography" analyzes the retention behavior of a sample over the temperature range of 5-80 degrees C during gradient elution reversed-phase high-performance liquid chromatography. Because this technique greatly increases the effective concentration of analyte upon adsorption to the column, it is extremely sensitive, requiring very small sample quantities (microgram or less). In contrast, the classical techniques mentioned above decrease the effective analyte concentration during analysis, decreasing sensitivity by requiring larger amounts of analyte to detect molecular self-association. We demonstrate the utility of this technique with 14-residue cyclic and linear cationic peptides (<2000 Da) based on the sequence of the de novo-designed cytolytic peptide, GS14. The only requirements for the analyte molecule when using this technique are its ability to be retained on the reversed-phase column and to be subsequently removed from the column during gradient elution.
Dense Ceramic Membranes for Oxygen Separation (2019)

This chapter provides an overview of major membrane concepts and materials. It discusses besides membranes made from a mixed ionic–electronic conducting, other membranes incorporating an oxygen ion conductor and presents data from oxygen permeability measurements on selected membrane materials. The rate at which oxygen permeates through a nonporous ceramic membrane is essentially controlled by two factors: the rate of solid state diffusion within the membrane and that of interfacial oxygen exchange on either side of the membrane. The basic assumption of the theory is that the lattice diffusion of oxygen or the transport of electronic charge carriers through the bulk oxide determines the rate of overall oxygen permeation. Besides the possibility of surface exchange limitations, oxygen transport through dense ceramics is necessarily influenced by the presence of high-diffusivity paths along internal surfaces such as grain boundaries.
Treatment of Allergic Asthma with Monoclonal Anti-IgE Antibody (1999)

A recombinant humanized monoclonal antibody directed against IgE has potential as a treatment for subjects with moderate or severe allergic asthma.