gebhart/patrepeval huggingface dataset:
Effects of Pharmacological and Nonpharmacological Treatments on Thyroid Hormone Metabolism and Concentrations in Rat Brain (2000)

The activities of the 5I-deiodinase (5D-I), 5II deiodinase (5D-II) and 5III-deiodinase (5D-III) isoenzymes and tissue concentrations of thyroxine (T 4 ) and triiodothyronine (T 3 ) were measured in up to 10 regions of the rat brain after acute and subchronic nonpharmacological (sleep deprivation, 12 h fasting, 14 days' calorie-reduced diet) and pharmacological (ethanol, haloperidol, clozapine, lithium, carbamazepine, desipramine, fluoxetine, tranylcypromine, and mianserin) treatments. All of these treatments induced significant and sometimes dramatic changes in 5D-II activities and tissue concentrations of thyroid hormones and, to a lesser extent, in 5D-III activity. The activity of 5D-I remained unaffected. The results revealed a surprising specificity for each type of treatment in terms of the isoenzyme and hormone affected, the direction of the change, the brain region af-fected and the time of day. The changes in thyroid hormone concentrations frequently failed to correspond in any way to those in deiodinase activities and unexpected effects such as inhibition of both 5D-II and 5D-III were seen, indicating that there may be additional pathways of iodothyronine metabolism in the CNS. In conclusion, particularly 5D-II activity and thyroid hormone concentrations in the CNS are highly sensitive to many different kinds of influence that may induce changes in neuronal activity. However, these changes in deiodinase activities do not ensure stable tissue concentrations of T 3 , but were, on the contrary, in most cases accompanied by marked changes T 3 levels in the tissue. The implications of these findings for the physiological role of thyroid hormones in the CNS are discussed.
Distributed feature selection using vertical partitioning for high dimensional data (2016)

Feature selection is one of the most significant steps in machine learning that reduces the features space in order to achieve faster learning and yielding simpler models with high accuracy and interpretability. With rapid development in technologies, large scale high dimensional datasets are common today which degrades the performance of traditional feature selection techniques as they suffer with the scalability issues. Parallel feature selection is an obvious solution to deal with this problem. Due to advent of many distributed computing frameworks scalable computing has become a viable strategy in reference to feature selection. Present work proposes a distributed parallel feature selection technique that employs vertical distribution strategy for dataset to exploit parallel computation. It uses information gain filter based ranking method which evaluates multiple disjoint feature subsets of dataset in parallel. The key idea is the distribution of evaluation and rank generation of features over several computing nodes in parallel. Experiments are performed on multiple large scale and high dimension datasets and significant reduction in overall computation time is achieved.
Electric field dependence of d/sub h/ in lead magnesium niobate-lead titanate ceramics (2002)

The hydrostatic piezoelectric coefficient d/sub h/, in the 0.9PMN-0.1PT ferroelectric relaxor composition was studied as a function of the electric bias field at 500 Hz using a laser interferometer. It was found that d/sub h/ approaches a very large value of approximately 300 pC/N and then saturates at an electric bias field of approximately 3.5 kV/cm. The voltage coefficient and the sensitivity were also found to be comparable to and larger than, respectively, those in lead zirconate-titanate (PZT) ceramics despite a high dielectric constant in the relaxor material. Unlike the d/sub 33/ and d/sub 31/ piezoelectric coefficients, d/sub h/ did not, under the experimental conditions, depend on electric bias field in a manner predicted by the phenomenological theory.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
ADAM 12, a Disintegrin Metalloprotease, Interacts with Insulin-like Growth Factor-binding Protein-3 (2000)

Insulin-like growth factor-binding protein (IGFBP)-3 binds the insulin-like growth factors with high affinity and modulates their actions. Proteolytic cleavage of IGFBP-3 may regulate insulin-like growth factor bioavailability. IGFBP-3 is extensively degraded in serum during pregnancy; however, as yet the pregnancy-specific protease, or proteases, have not been identified. We utilized a yeast two-hybrid assay and a human placental cDNA library to investigate IGFBP-3-interacting proteins. A disintegrin and metalloprotease-12 (ADAM 12), a member of a family of metalloprotease disintegrins that is highly expressed in placental tissue, was identified as interacting with IGFBP-3. This interaction involved the cysteine-rich domain of ADAM 12. Unlike other members of this family of disintegrin metalloproteases that are membrane proteins, ADAM 12 exists as an alternatively spliced soluble secreted protein. To verify the interaction between ADAM 12 and IGFBP-3, an expression construct containing an ADAM 12-S cDNA was transfected into COS-1 cells. Co-precipitation was observed when conditioned medium was analyzed by immunoprecipitation with an antibody against either ADAM 12 or IGFBP-3 followed by Western blotting with anti-IGFBP-3 or anti-ADAM 12. Although minimal proteolysis of IGFBP-3 was observed in conditioned medium from control cells, this was increased approximately 4-fold in conditioned medium from ADAM 12-S-transfected cells. Recombinant ADAM 12-S partially purified from conditioned medium on a heparin-Sepharose column also proteolyzed IGFBP-3. The degradation pattern was similar to that seen with pregnancy serum, and the presence of ADAM 12-S in serum during pregnancy was confirmed. The data suggest that ADAM 12-S has IGFBP-3 protease activity, and it may contribute to the IGFBP-3 protease activity present in pregnancy serum.
Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody. (1983)

The distribution and localization of a glioma-associated antigen defined by monoclonal antibody 81C6 has been examined using human cultured cell lines and tissues. Monoclonal antibody 81C6 was selected from a hybridoma fusion of spleen cells of mice immunized with the glial fibrillary acidic protein-positive human glioma cell line U-251 MG. Results of cell surface radioimmunoassay and absorption analysis demonstrated that 81C6 defined a glioma-mesenchymal extracellular matrix (GMEM) antigen expressed by 14 of 16 gliomas, 1 of 3 neuroblastomas, 1 of 7 melanomas, 2 of 6 sarcoma cell lines, and 8 of 9 cultured fibroblast lines. GMEM was not expressed by carcinoma or by the myeloid-lymphoid cell lines examined. Within the central nervous system, GMEM was expressed in 10 of 11 glioblastomas but was undetected in 5 of 6 astrocytomas and in normal adult and fetal brain by peroxidase-antiperoxidase immunohistology. In glioblastomas, the GMEM antigen was localized to basement membranes of the distinctive glomeruloid endothelial proliferations and hyperplastic blood vessels. The GMEM antigen was also expressed in 3 of 3 glioblastoma cell lines and 6 of 8 glioblastoma biopsy xenografts in athymic nude mice. Among non-central nervous system tissues and tumors, GMEM was found by peroxidase-antiperoxidase immunohistology in normal liver sinusoids, spleen red pulp sinusoids, kidney medullary tubule interstitium, and glomerular mesangium and in association with vascular and stromal elements of several undifferentiated tumors. The GMEM antigen is distinct from previously described forms of fibronectin, laminin, collagen types I to V, hyaluronic acid, chondroitin sulfate, and heparin, as determined by absorption analysis and immunohistological localization in tissues. The expression of GMEM in glioblastoma but not normal brain, association with glioblastoma-proliferative endothelium basement membranes, and expression in glioblastoma cell lines and nude mouse xenografts suggest that GMEM may be a useful marker of gliomas in vivo and in vitro.
Major changes in the G protein of human respiratory syncytial virus isolates introduced by a duplication of 60 nucleotides (2003)

The entire nucleotide sequence of the G gene of three human respiratory syncytial virus (HRSV) isolates (antigenic group B) has been determined. These three viruses (named BA viruses) were isolated in Buenos Aires in 1999 from specimens collected in different hospitals and at different dates. BA viruses have an exact duplication of 60 nucleotides in the G gene, starting after residue 791. This duplication is flanked by a repeat of four nucleotides (GUGU) and can fold into a relatively stable secondary structure. These features suggest a possible mechanism for the generation of a duplicated G segment. The predicted polypeptide is lengthened by 20 amino acids (residues 260-279) and this is reflected in the slower electrophoretic mobility of the G protein precursor of BA viruses compared with related viruses. The changes reported here expand the examples of drastic genetic alterations that can be introduced into the G protein sequence of HRSV while it replicates in its natural host.
A Low-Power Low-Cost 24 GHz RFID Tag With a C-Flash Based Embedded Memory (2014)

<?Pub Dtl=""?> The key factor in widespread adoption of Radio Frequency Identification (RFID) technology is tag cost minimization. This paper presents the first low-cost, ultra-low power, passive RFID tag, fully integrated on a single substrate in a standard CMOS process. The system combines a 24 GHz, dual on-chip antenna, RF front-end, and a C-Flash based, rewritable, non-volatile memory module to achieve full on-chip system integration. The complete system was designed and fabricated in the TowerJazz 0.18 µm CMOS technology without any additional mask adders. By embedding the RF, memory, and digital components together upon a single substrate in a standard digital process, the low-cost aspirations of the “5-cent RFID tag” become feasible. Design considerations, analysis, circuit implementations, and measurement results are presented. The entire system was fabricated on a 3.6 mm × 1.6 mm (6.9 mm 2 ) die with the integrated antennas comprising 82% of the silicon area. The total read power was measured to be 13.2 µW, which is sufficiently supplied by the on-chip energy harvesting unit.
Domain Specific Parallel Crawler Architecture (2016)

The World Wide Web is an interlinked gathering of billions of reports organized utilizing HTML. Because of the developing and dynamic nature of the web, it has turned into a test to cross all URLs in the web archives and handle these URLs, so it has ended up basic to parallelize a creeping prepare. The crawler process is further being parallelized in the shape nature of crawler specialists that parallel download data from the web. This paper proposes a novel engineering of parallel crawler, which depends on area particular creeping, makes slithering assignment more compelling, versatile and load-sharing among the distinctive crawlers which parallel download website pages identified with various spaces particular URLs. Keywords : URL (uniform resource locator), URI (uniform resource identifier), crawl work Cite this Article Himanshu Verma. Domain Specific Parallel Crawler Architecture. Recent Trends in Parallel Computing. 2016; 3(1): 17–21p.
A Role of Dystroglycan in Schwannoma Cell Adhesion to Laminin (1997)

Dystroglycan is encoded by a single gene and cleaved into two proteins alpha- and beta-dystroglycan by posttranslational processing. Recently, alpha-dystroglycan was demonstrated to be an extracellular laminin-binding protein anchored to the cell membrane by a transmembrane protein beta-dystroglycan in striated muscle and Schwann cells. However, the biological functions of the dystroglycan-laminin interaction remain obscure, and in particular, it is still unclear if dystroglycan plays a role in cell adhesion. In the present study, we characterized the role of dystroglycan in the adhesion of schwannoma cells to laminin-1. Immunochemical analysis demonstrated that the dystroglycan complex, comprised of alpha- and beta-dystroglycan, was a major laminin-binding protein complex in the surface membrane of rat schwannoma cell line RT4. It also demonstrated the presence of alpha-dystroglycan, but not beta-dystroglycan, in the culture medium, suggesting secretion of alpha-dystroglycan by RT4 cells. RT4 cells cultured on dishes coated with laminin-1 became spindle in shape and adhered to the bottom surface tightly. Monoclonal antibody IIH6 against alpha-dystroglycan was shown previously to inhibit the binding of laminin-1 to alpha-dystroglycan. In the presence of IIH6, but not several other control antibodies in the culture medium, RT4 cells remained round in shape and did not adhere to the bottom surface. The adhesion of RT4 cells to dishes coated with fibronectin was not affected by IIH6. The known inhibitors of the interaction of alpha-dystroglycan with laminin-1, including EDTA, sulfatide, fucoidan, dextran sulfate, heparin, and sialic acid, also perturbed the adhesion of RT4 cells to laminin-1, whereas the reagents which do not inhibit the interaction, including dextran, chondroitin sulfate, dermatan sulfate, and GlcNAc, did not. Altogether, these results support a role for dystroglycan as a major cell adhesion molecule in the surface membrane of RT4 cells.
Surface Characterization and Functionalization of MCM-41 Silicas via Silazane Silylation (2000)

Disilazane reagents of type HN(SiR1R22)2 carrying organic substituents R1,2 = H, Me, Ph, n-Bu, n-Oct, Vin (Vin = vinyl) of varying longitudinal and lateral extension are reacted with high quality MCM-41 samples of different pore sizes, i.e., effective pore diameters of 2.8 and 3.8 nm according to the BJH pore size analysis of the desorption branch. The reaction of the standard silylating reagent hexamethyldisilazane, HN(SiMe3)2, is shown to be controlled by the amount of added silylamine and the contact time, resulting in effective pore size engineering. Calculations from elemental analysis revealed that the degree of silylation (silylation efficiency) and hence the surface hydroxyl consumption depend on the steric bulk/shape of the groups R. The surface coverage varies from 0.74 to 1.85 silyl groups/nm2. The sterically least demanding tetramethyldisilazane, HN(SiHMe2)2, is the most efficient silylating reagent, while silyl groups with bulky phenyl substituents produce the lowest surface coverage featuring considerable interaction with nonsilylated silanol groups. The formation of various covalently linked siloxy functionalities [OSiR1R22] is reflected in the change of the pore volume and mean pore diameter as evidenced by nitrogen physisorption measurements at 77.4 K. A monofunctional surface reaction and the structural integrity of the immobilized (functionalized) silyl groups is quantitatively revealed by means of FT IR and 1H/13C MAS NMR spectroscopy. Heterobisilylated organic/inorganic hybrid materials are synthesized both via consecutive and competitive silylation utilizing mixtures of silylamines. The latter silylation procedure provides important mechanistic and kinetic details of this peculiar surface silylation reaction emphasizing the preformation of a four-centered “O···H···N···Si” transposition as the rate-determining step. Fully silylated materials carrying reactive vinyl moieties were functionalized by hydroboration with BH3(THF) and 9-BBN.
Electroporation Protocols for Agrobacterium (2003)

Agrobacterium spp. are widely used to transform new genes into plant tissue. Transferring useful genes into bacteria in order to transform them into the target plant is an essential part of the process, which can be achieved by electroporation.
Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity (2006)

Control of the establishment of cell polarity is an essential function in tissue morphogenesis and renewal that depends on spatial cues provided by the extracellular environment. The molecular role of cell-cell or cell-extracellular matrix (ECM) contacts on the establishment of cell polarity has been well characterized. It has been hypothesized that the geometry of the cell adhesive microenvironment was directing cell surface polarization and internal organization. To define how the extracellular environment affects cell polarity, we analyzed the organization of individual cells plated on defined micropatterned substrates imposing cells to spread on various combinations of adhesive and nonadhesive areas. The reproducible normalization effect on overall cell compartmentalization enabled quantification of the spatial organization of the actin network and associated proteins, the spatial distribution of microtubules, and the positioning of nucleus, centrosome, and Golgi apparatus. By using specific micropatterns and statistical analysis of cell compartment positions, we demonstrated that ECM geometry determines the orientation of cell polarity axes. The nucleus-centrosome orientations were reproducibly directed toward cell adhesive edges. The anisotropy of the cell cortex in response to the adhesive conditions did not affect the centrosome positioning at the cell centroid. Based on the quantification of microtubule plus end distribution we propose a working model that accounts for that observation. We conclude that, in addition to molecular composition and mechanical properties, ECM geometry plays a key role in developmental processes.
Comparison of IL-10 and MCP-1-7ND gene transfer with AAV9 vectors for protection from murine autoimmune myocarditis (2011)

Our study suggests that cardiac expression of IL-10 with AAV9 vectors is a promising therapeutic approach for autoimmune myocarditis.
Monosodium urate crystals stimulate phospholipase A2 enzyme activities and the synthesis of a phospholipase A2-activating protein. (1990)

Eicosanoids are important mediators of the inflammatory response to monosodium urate crystals (MSUC) that results in gout. Phospholipase enzymes cleave fatty acids from membrane phospholipids, and this is thought to be the rate-limiting step in eicosanoid production. To understand better the mechanism of eicosanoid production in this disease, we stimulated human peripheral blood neutrophils and monocytes with MSUC and measured phospholipase enzyme activities. MSUC stimulated both intracellular and secretory phospholipase A2 enzyme activities in a time and concentration-dependent manner. Specificity was observed, as phospholipase C activities were not affected. Pretreatment with colchicine, but not aspirin, indomethacin, allopurinol, or islet activating protein, abrogated the enhanced phospholipase A2 activities. We have recently isolated and characterized a phospholipase A2 activating protein termed PLAP from synovial fluid from patients with rheumatoid arthritis, and from murine and bovine cell lines. PLAP was detected in gouty synovial fluid by immunodot blotting and ELISA assays and expressed the same characteristics as PLAP identified from other sources. To examine the role of PLAP in MSUC-induced phospholipase A2 stimulation, we treated cells with MSUC and observed an increase in immunoreactive PLAP. This response also could be blunted by colchicine, but not other drugs. Both phospholipase A2 and PLAP induced production by human monocytes of PGE2 and leukotriene B4 by neutrophils. These findings suggest that phospholipase A2 activation in response to MSUC requires an intact microtubule structure, and that phospholipase A2 and PLAP may be important modulators of at least a portion of the gouty inflammatory response.
Enzyme Dynamics During Catalysis (2002)

Internal protein dynamics are intimately connected to enzymatic catalysis. However, enzyme motions linked to substrate turnover remain largely unknown. We have studied dynamics of an enzyme during catalysis at atomic resolution using nuclear magnetic resonance relaxation methods. During catalytic action of the enzyme cyclophilin A, we detect conformational fluctuations of the active site that occur on a time scale of hundreds of microseconds. The rates of conformational dynamics of the enzyme strongly correlate with the microscopic rates of substrate turnover. The present results, together with available structural data, allow a prediction of the reaction trajectory.
Purification and characterization of a membrane-bound and a secreted mucin-type glycoprotein carrying the carcinoma-associated sialyl-Lea epitope on distinct core proteins. (1991)

Two mucin-type glycoproteins detected by the monoclonal antibody C50, which reacts with the carcinoma-associated sialyl-Lewis a and sialyl-lactotetraose epitopes, were found in secreted and solubilized materials from the colon carcinoma cell line COLO 205. The larger glycoprotein (H-CanAg; heavy cancer antigen) was predominantly found in extracts of cells grown in vitro or as nude mice xenografts whereas the smaller species (L-CanAg; light cancer antigen) was the major component in spent culture medium and serum from grafted mice. Using detergent in the extraction buffer doubled the yield of H-CanAg, suggesting that this glycoprotein is membrane bound whereas the yield of L-CanAg was relatively unaffected. The two glycoproteins were purified from xenograft extracts and spent culture medium using perchloric acid precipitation, monoclonal antibody affinity purification, ion exchange chromatography, and gel filtration. Both glycoproteins were unaffected by reduction and alkylation in guanidine HCl. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, relative molecular masses were estimated to be 600-800 kDa for H-CanAg and 150-300 kDa for L-CanAg. Carbohydrate analysis revealed that the CanAg glycoproteins were highly glycosylated (81-89% carbohydrate by weight), carrying carbohydrate chains with average lengths of 13-18 sugars which were rich in fucose and sialic acid (2-3 residues/chain for each sugar). L-CanAg isolated from spent medium was glycosylated to a higher degree than its counterpart from xenograft extract. Immunochemical studies of the intact glycoproteins showed that both H-CanAg and L-CanAg expressed the monoclonal antibody-defined, sialic acid-containing carbohydrate epitopes CA203 and CA242 as well as the Lewis a blood group antigen whereas only H-CanAg appeared to carry the sialyl-Lewis x epitope. The amino acid compositions were typical of mucins, containing high amounts of serine, threonine (more than 25% together), and proline (11-18%). Significant differences in amino acid composition between H-CanAg and L-CanAg were found. A rabbit antiserum against the cytoplasmic C-terminal part of the MUC1 gene product, core protein of the carcinoma-associated polymorphic epithelial mucin (PEM) and DU-PAN-2, reacted with H-CanAg. After deglycosylation with trifluoromethanesulfonic acid, H-CanAg but not L-CanAg was recognized by the monoclonal antibodies SM-3 and HMFG-2, directed to the tandem repeat of the PEM apoprotein. However, these antibodies which react with PEM from mammary carcinomas without prior deglycosylation were unable to recognize intact H-CanAg, probably as a consequence of a more extensive glycosylation of this glycoprotein.(ABSTRACT TRUNCATED AT 400 WORDS)
Accuracy of the pleth variability index to predict fluid responsiveness depends on the perfusion index (2011)

The PVI was not able to predict fluid responsiveness with sufficient accuracy. In patients with higher perfusion states, the PVI improved its ability to predict haemodynamic changes, strongly suggesting a relevant influence of the PI on the PVI.