gebhart/patrepeval huggingface dataset:
Bone‐specific heparan sulfates induce osteoblast growth arrest and downregulation of retinoblastoma protein (2006)

The heparan sulfate (HSs) sugars of the extracellular matrix (ECM) play a key role during both development and wound repair in regulating the flow of growth and adhesive factors across their cell surface receptors. The aim of this study was to assess the structural and functional differences of HS chains extracted from the conditioned media (soluble), cell surface, and ECM of primary human osteoblast cultures, and to analyze their effects on osteoblast cell growth. HS chains from these compartments were characterized through a combination of enzymatic degradation, anion exchange chromatography, and molecular sieving. Although the chains were all approximately the same size, they varied systematically in their sulfate content, suggesting differences in their protein-binding domains. When added to pre-confluent hFOB1.19 osteoblast cultures, HS doses exceeding 500 ng/ml inhibited proliferation, without affecting viability, irrespective of their origin. Furthermore, HS doses of 500 ng/ml also downregulated retinoblastoma, Cyclin A and CDK1 protein expression, indicating that high doses of osteoblast HS negatively regulate cell cycle, resulting in growth arrest; when high doses of HS were withdrawn after a prolonged period, linear cell growth was reestablished. Thus, despite differences in sulfation, HS from either the soluble, cell surface, or matrix compartments of primary human osteoblast cultures are functionally similar with respect to their effects on growth. Binding assays revealed that the HS chains bound TGFbeta1, a known inhibitor of osteoprogenitor growth, at higher affinity than a suite of other bone-related, heparin-binding growth factors. Overcoming such sugar-mediated inhibition may prove important for wound repair.
A high-resolution high-frequency monolithic top-shooting microinjector free of satellite drops - part I: concept, design, and model (2002)

Introduces an innovative microinjector design, featuring a bubble valve, which entails superior droplet ejection characteristics and monolithic fabrication, which allows handling of a wide range of liquids. This new microinjector uses asymmetric bubbles to reduce crosstalk, increase frequency response and eliminate satellite droplets. During a firing, i.e., droplet ejection, the "virtual valve" closes, by growing a thermal bubble in the microchannel, to isolate the microchamber from the liquid supply and neighboring chambers. Between firings, however, the virtual valve opens, by collapsing the bubble, to reduce flow restriction for fast refilling of the microchamber. The use of bubble valves brings about fast and reliable device operation without imposing the significant complication fabrication of physical microvalves would call for. In addition, through a special heater configuration and chamber designs, bubbles surrounding the nozzle cut off the tail of the droplets being ejected and completely eliminate satellite droplets. A simple one-dimensional model of the operation of the microinjector is used to estimate the bubble formation and liquid refilling.
Phase Transfer‐Catalyzed Fast CO2 Absorption by MgO‐Based Absorbents with High Cycling Capacity (2014)

Molten salts facilitate the reaction of CO2 with MgO by providing an alternate pathway to traditional gas-solid reactions. Molten salts partially dissolve bulk MgO and provide activated species accessible to CO2 at gas-solid-liquid triple phase boundaries. This methodology is also applicable to other basic metal oxides and molten salts, inspiring the design of new absorbent systems.
PRELIMINARY EXPERIENCE WITH α-2b-INTERFERON THERAPY OF VIRAL HEPATITIS IN LIVER ALLOGRAFT RECIPIENTS1,2 (1992)

Currently, alpha interferon is the only recognized therapy for chronic viral hepatitis. As a result of its success in several multicenter trials, the agent was approved recently by the FDA for use in the clinical management of patients with chronic hepatitis C. FDA approval for its use in chronic hepatitis B is anticipated. Based upon this experience in nonimmunosuppressed individuals, the efficacy of alpha interferon therapy in patients who are recipients of liver allografts and are receiving chronic immunosuppression was assessed in a preliminary trial of the agent in 30 patients (13 with HBV, 11 with HCV, and 6 with hepatitis non A, non B, non C). Therapy was initiated at a dose of 3 X 10(6) units three times per week and continued for 6 months. Dose reduction in the amount of the alpha interferon administered was determined by a preestablished protocol. Nine percent of those with HCV and 18% of those with hepatitis non A, non B, non C experienced a full response to alpha interferon therapy. No full responses to alpha interferon therapy. No full responses were seen in those with HBV disease. Partial responses were common in all three groups but were most frequent in those with hepatitis non A, non B, non C and least frequent in those with HCV-related disease. This preliminary experience demonstrates the following: 1. Viral hepatitis following OLTx can be treated with alpha-2b-interferon. 2. The complications of alpha-2b-interferon therapy utilized prior to OLTx can be avoided by giving the therapy following successful OLTx. 3. The high rate of partial responses noted suggests that future studies should utilize either higher doses or longer durations of therapy or both. 4. The response rate was greatest for those having non A, non B, non C hepatitis and least for those with HCV hepatitis. 5. In this small preliminary series, no episodes of liver graft rejection could be ascribed to the use of alpha-2b-interferon in the patients so treated.
Antitumor effect of arginine butyrate in conjunction with corynebacterium parvum and interferon (1982)

The antitumor effect of arginine butyrate, used alone or in conjunction with interferon (IFN), was studied using randomly selected Swiss mice inoculated i.p. with 10(6) 180 TG Crocker tumor cells. The results of these different therapeutic regimens were estimated by tumor incidence at 10 days, by the time necessary to protect 50% of the animals, and by the final survival rate. Combined treatment by IFN and arginine butyrate was effective; but in order to compensate for IFN's immune repressive effects, a single injection of Corynebacterium parvum was administered in some experiments prior to IFN and/or arginine butyrate. After this immune potentiation, both IFN and arginine butyrate used separately significantly protected the animals. However, optimal results were obtained when a single C. parvum injection was followed by nine daily alternating shots of arginine butyrate and IFN.
Deciphering Protein Complexes and Protein Interaction Networks by Tandem Affinity Purification and Mass Spectrometry (2002)

We employed a combination of tandem affinity purification and mass spectrometry for deciphering protein complexes and the protein interaction network in budding yeast. 53 genes were epitope-tagged, and their interaction partners were isolated by two-step immunoaffinity chromatography from whole cell lysates. 38 baits pulled down a total of 220 interaction partners, which are members of 19 functionally distinct protein complexes. We identified four proteins shared between complexes of different functionality thus charting segments of a protein interaction network. Concordance with the results of genome-wide two-hybrid screening was poor (14% of identified interactors overlapped) suggesting that the two approaches may provide complementary views on physical interactions within the proteome.
Arytenoid Repositioning Device (2014)

We confirm the feasibility of minimal-access arytenoid adduction and abduction through development of a new technique and device.
Handbook of Research on Culturally-Aware Information Technology: Perspectives and Models (2010)

"This book provides readers with the possibility of acquiring in-depth knowledge of the theoretical and technological research conducted in IT in relation to culture" - Provided by publisher
Femtosecond Laser Photodisruption of Primate Trabecular Meshwork: An Ex Vivo Study (2009)

The present study demonstrates that laser ablation of the TM ab interno in ex vivo primate eyes is feasible by a custom femtosecond laser ablation system with a gonioscopic lens.
The International Collaborative Group on Hereditary Non-Polyposis Colorectal Cancer (ICG-HNPCC) (1991)

The meeting was financially supported by the Foundation for the Detection of Hereditary Tumours, the National Cancer Society, and EUROFAP.
Selecting motors for robots using biomimetic trajectories: Optimum benchmarks, windings, and other considerations (2010)

Robotic actuators must frequently be designed to provide both substantial torque and acceleration in a lightweight package. Their ballistic trajectories require new optimization guidelines, in contrast to constant-speed or low-torque conventional motor design. A framework was derived in this study based on trajectories that incorporated the effect of velocity on torque and acceleration. This framework suggested that speed rate (or mechanical motor constant) was the best benchmark of robotic motor performance. Implications of this framework were evaluated using simulations of 37 optimized motor models. Simulation results confirmed the framework predictions, suggesting that speed rate was a better predictor of motor success than either motor constant or rated power. Optimum winding/gear ratio, supply voltage, and implications for design choices such as continuously variable transmissions are discussed in light of these findings.
Childhood Acute Myelomonocytic Leukemia (AML-M4) Presenting as Catastrophic Antiphospholipid Antibody Syndrome (2004)

: The authors describe a 15-year-old girl presenting with a cerebral ischemic stroke as the first manifestation of catastrophic antiphospholipid antibody syndrome secondary to acute myeloid leukemia (AML). Despite treatment with anticoagulants, therapeutic plasma exchange, and chemotherapy, the patient developed multiorgan thromboses and failure, eventually culminating in death. This unusual presentation of AML has not been previously described in children. Clinical features of antiphospholipid antibody syndrome and current knowledge regarding its association with malignancies are reviewed.
Modulation bandwidth enhancement and nonlinear distortion suppression in directly modulated monolithic injection-locked DFB lasers (2005)

We have experimentally investigated the dynamic performance and the suppression of nonlinear distortions of a monolithic optical injection-locked distributed feedback (DFB) laser. The master and the slave lasers are monolithically integrated in a single strip of strongly coupled DFB laser. Optical injection locking is achieved by current tuning. The resonant frequency of the laser is increased from 11 GHz to 23 GHz, and nonlinear distortions are suppressed by more than 15 dB.
Detection ofAspergillusSpecies in Blood and Bronchoalveolar Lavage Samples from Immunocompromised Patients by Means of 2‐Step Polymerase Chain Reaction: Clinical Results (2001)

Bronchoalveolar lavage (BAL) samples from 67 patients who were at high risk for invasive aspergillosis were examined using a recently developed 2-step polymerase chain reaction (PCR) that detects </=10 fg of Aspergillus DNA in blood and BAL samples in vitro. Thirteen of these patients had PCR and diagnostic results positive for Aspergillus infection. Four patients with possible invasive aspergillosis also had positive PCR results, and the remaining 50 had negative PCR results. In addition, 907 blood samples from 218 high-risk patients were screened. Thirty-three patients with positive PCR results had invasive aspergillosis; 148 patients had PCR and diagnostic results that were negative, and 34 patients with positive PCR results had nonconclusive clinical data. Both blood and BAL testing were performed for 45 patients. All 8 patients with proven invasive aspergillosis showed concordance of positive PCR results. Our data suggest that this PCR method has possible clinical value for confirming and improving the diagnosis of invasive aspergillosis in high-risk patients.
Photoirradiated Atom Transfer Radical Polymerization with an Alkyl Dithiocarbamate at Ambient Temperature (2010)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTPhotoirradiated Atom Transfer Radical Polymerization with an Alkyl Dithiocarbamate at Ambient TemperatureYungwan Kwak and Krzysztof Matyjaszewski*View Author Information Center for Macromolecular Engineering, Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213*Corresponding author: Tel +1-412-268-3209; e-mail [email protected]Cite this: Macromolecules 2010, 43, 12, 5180–5183Publication Date (Web):May 27, 2010Publication History Received17 April 2010Revised18 May 2010Published online27 May 2010Published inissue 22 June 2010https://doi.org/10.1021/ma100850aCopyright © 2010 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views2497Altmetric-Citations128LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (998 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Group 11 complexes,Irradiation,Polymerization,RAFT polymerization,Radical polymerization Get e-Alerts
Catalytic Domain Structure of Vampire Bat Plasminogen Activator:  A Molecular Paradigm for Proteolysis without Activation Cleavage (1997)

The saliva of the blood-eating vampire bat Desmodus rotundus contains plasminogen activators (PAs) that maintain the fluidity of the prey's blood by activating plasminogen and dissolving developing fibrin clots. D. rotundus salivary PAs (DSPAs) are composed of evolutionarily conserved domains reminiscent of human tissue-type PA (tPA), but their catalytic domain lacks a plasmin-sensitive "activation cleavage site". Despite this, all DSPAs are intrinsically active and enormously stimulated in the presence of fibrin. The recombinant catalytic domain of DSPAalpha1 has been crystallized in a covalent complex with Glu-Gly-Arg-chloromethyl ketone and its structure solved at 2.9 A resolution. The structure is similar to that of activated two-chain human tPA. Despite its single-chain status, the activation domain is observed in an enzymatically active conformation, with a functional substrate binding site and active site accommodating the peptidylmethylene inhibitor. The activation pocket, which normally receives the N-terminal Ile16, is occupied by the side chain of Lys156, whose distal ammonium group makes an internal salt bridge with the carboxylate group of Asp194. Lys156 is in a groove shielded from the bulk solvent by the intact "activation loop" (Gln10-Phe21), favoring Lys156-Asp194 salt bridge formation and stabilization of a functional substrate binding site. Together with the characteristic 186 insertion loop, the activation loop could act as a switch, effecting full single-chain enzymatic activity upon binding to fibrin.
<title>Extraction of text-related features for condensing image documents</title> (1996)

A system has been built that selects excerpts from a scanned document for presentation as a summary, without using character recognition. The method relies on the idea that the most significant sentences in a document contain words that are both specific to the document and have a relatively high frequency of occurrence within it. Accordingly, and entirely within the image domain, each page image is deskewed and the text regions of are found and extracted as a set of textblocks. Blocks with font size near the median for the document are selected and then placed in reading order. The textlines and words are segmented, and the words are placed into equivalence classes of similar shape. The sentences are identified by finding baselines for each line of text and analyzing the size and location of the connected components relative to the baseline. Scores can then be given to each word, depending on its shape and frequency of occurrence, and to each sentence, depending on the scores for the words in the sentence. Other salient features, such as textblocks that have a large font or are likely to contain an abstract, can also be used to select image parts that are likely to be thematically relevant. The method has been applied to a variety of documents, including articles scanned from magazines and technical journals.
A double-blind, randomized quantitative comparison of calcitriol ointment and calcipotriol ointment on epidermal cell populations, proliferation and differentiation (2006)

Both calcitriol and calcipotriol gave a significant improvement in clinical scores. However, treatment with calcipotriol resulted in a normalization of K6, K10 and K15, whereas treatment with calcitriol did not. Comparison of both treatments showed a tendency towards significance for the above-mentioned markers for calcipotriol only.
Molecular Design, Device Function and Surface Potential of Zwitterionic Electron Injection Layers (2009)

A series of zwitterionic molecules were synthesized by addition of iodoalkanes to sodium tetrakis(1-imidazolyl)borate (NaBIm(4)). Single substitution at the N3 site leads to compounds designated as C(n)-BIm(4) (n = 1, 6, 12, 16), where the subscript corresponds to the number of carbon atoms in the linear alkyl chain. Metrical parameters in the molecular structure of C(1)-BIm(4) determined by single crystal X-ray diffraction studies confirms the zwitterionic nature and were used to calculate the molecular dipole moment. Compounds C(n)-BIm(4) can be used to improve the electroluminescence efficiencies of polymer light emitting diodes (PLEDs) fabricated by simple solution methods that use poly[2-methoxy-5-(2'-ethylhexyloxy-1,4-phenylenevinylene)] (MEH-PPV) as the emissive semiconducting layer and aluminum as the cathode. This improvement is primarily due to a reduction in the barrier to electron injection and is strongly dependent on the length of the alkyl substituent in C(n)-BIm(4), with C(16)-BIm(4) yielding the best performance. Examination of the surface topography of C(1)-BIm(4) and C(16)-BIm(4) atop MEH-PPV by atomic force microscopy (AFM) shows that the longer alkyl chains assist in adhesion to the surface, however the coverage is poor due to insufficient wetting. These studies also reveal an unexpected planarization of the C(16)-BIm(4) layer adjacent to the electrodes after metal deposition, which is suggested to improve electrical contact. A combination of open circuit voltage measurements, surface reconstruction studies and surface potential measurements indicates that zwitterion insertion leads to the formation of a spontaneously organized dipole layer at the metal/organic interface. The net effect is a shift in the vacuum level and concomitant improvement of electron injection.
Novel intercalators of pyrylium dye into double-stranded DNA. (1993)

Discovery is reported of novel intercalators without fused ring systems, pyrylium and thiopyrylium salt. 2-Methyl-4,6-bis-(N,N-dimethylaminophenyl) pyrylium salt 1 (Fig. 1) is, in particular, virtually non-fluorescent in aqueous solution, but form highly fluorescent complexes with double-stranded DNA (dsDNA), which yield more than 400 fold fluorescence enhancement at the peak emission. We report the synthesis, absorption and fluorescence properties of 1 and its thiopyrylium derivative 2 in comparison with other pyrylium 3, thiopyrylium 4 dyes and typical intercalator, ethidium bromide(EB). Compound 1 shows the absorption maximum at 540 nm and the red shift (about 40 nm) in the presence of dsDNA in similar to EB. The enhancement of fluorescence emission excited by visible light is extremely sensitive to the concentration of dsDNA comparing to that of EB. The thiopyrylium salt 2 with absorption maximum at 575 nm shows the similar profile in the absorption spectral shift and fluorescence enhancement to that of 1, when the dye binds to dsDNA.
Rhodium-Catalyzed Asymmetric 1,4-Addition of Aryl- and Alkenylboronic Acids to Enones (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTRhodium-Catalyzed Asymmetric 1,4-Addition of Aryl- and Alkenylboronic Acids to EnonesYoshiaki Takaya, Masamichi Ogasawara, Tamio Hayashi, Masaaki Sakai, and Norio MiyauraView Author Information Department of Chemistry, Graduate School of Science Kyoto University, Sakyo, Kyoto 606-8502, Japan Division of Molecular Chemistry Graduate School of Engineering Hokkaido University, Sapporo 060-8628, Japan Cite this: J. Am. Chem. Soc. 1998, 120, 22, 5579–5580Publication Date (Web):May 19, 1998Publication History Received2 March 1998Published online19 May 1998Published inissue 1 June 1998https://pubs.acs.org/doi/10.1021/ja980666hhttps://doi.org/10.1021/ja980666hrapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views12448Altmetric-Citations638LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Catalysts,Enones,Ligands,Rhodium,Stereoselectivity Get e-Alerts