gebhart/patrepeval huggingface dataset:
Long-lived, highly luminescent rhenium(I) complexes as molecular probes: intra- and intermolecular excited-state interactions (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLong-lived, highly luminescent rhenium(I) complexes as molecular probes: intra- and intermolecular excited-state interactionsLouAnn Sacksteder, Maria Lee, J. N. Demas, and B. A. DeGraffCite this: J. Am. Chem. Soc. 1993, 115, 18, 8230–8238Publication Date (Print):September 1, 1993Publication History Published online1 May 2002Published inissue 1 September 1993https://pubs.acs.org/doi/10.1021/ja00071a036https://doi.org/10.1021/ja00071a036research-articleACS PublicationsRequest reuse permissionsArticle Views897Altmetric-Citations204LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
TIC DISORDERS (1997)

The picture that emerges is one of complex behavioral phenotypes that alter over the course of CNS development. While many cases are mild and may not come to medical attention, others are chronic and disabling. The aim of this article is to provide an overview of recent progress in understanding phenomenology, epidemiology, genetics, neurobiology, and treatment of tic disorders.
Economic and herbicide use impacts of glyphosate‐resistant crops (2005)

More than 95% of United States maize, cotton, soybean and sugarbeet acres are treated with herbicides for weed control. These products are used to improve the economic profitability of crop production for farmers. Since their introduction in 1996, over 75 million acres of genetically engineered glyphosate-resistant crops have been planted, making up 80% of soybean acres and 70% of cotton acres in the USA. These genetically engineered crops have been adopted by farmers because they are perceived to offer greater economic benefits than conventional crop and herbicide programs. The adoption of glyphosate-resistant crops has saved US farmers 1.2 billion dollars associated with the costs of conventional herbicide purchases, application, tillage and hand weeding. With the adoption of glyphosate-resistant sugarbeets on currently planted sugarbeet acres, US growers could potentially save an additional 93 million dollars. The adoption of glyphosate-resistant crops by US agriculture has reduced herbicide use by 37.5 million lbs, although the adoption of glyphosate-resistant sugarbeets would dampen this reduction by 1 million lbs.
7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines (1968)

Research Article| June 01 1968 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines P B Ghosh; P B Ghosh 1Departments of Medical Chemistry and Experimental Pathology, John C(urtin School of Medical Research, The Australian National University, Canberra, A.C.T., Awstralia Search for other works by this author on: This Site PubMed Google Scholar M W Whitehouse M W Whitehouse 1Departments of Medical Chemistry and Experimental Pathology, John C(urtin School of Medical Research, The Australian National University, Canberra, A.C.T., Awstralia Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1968) 108 (1): 155–156. https://doi.org/10.1042/bj1080155 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation P B Ghosh, M W Whitehouse; 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines. Biochem J 1 June 1968; 108 (1): 155–156. doi: https://doi.org/10.1042/bj1080155 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1968 The Biochemical Society1968 Article PDF first page preview Close Modal You do not currently have access to this content.
Defective Suppressor Function in CD4+CD25+ T-Cells From Patients With Type 1 Diabetes (2005)

Type 1 diabetes is a T-cell-mediated disease that is associated with loss of immunological tolerance to self-antigens. The mechanisms involved in maintenance of peripheral tolerance include a specialized subset of regulatory T-cells (Treg) within the CD4(+)CD25(+) T-cell population, but the function and phenotype of these cells in type 1 diabetes have not been investigated. We hypothesized that a deficiency in the CD4(+)CD25(+) Treg population or its function could contribute to the lack of self-tolerance evident in patients with type 1 diabetes. We show that although levels of CD4(+)CD25(+) T-cells are normal in patients with recent-onset adult type 1 diabetes, the ability of the Tregs in this population to suppress T-cell proliferation during in vitro cocultures is markedly reduced compared with control subjects (P = 0.007). Moreover, in patients with type 1 diabetes, these cocultures display a more proinflammatory phenotype, with increased secretion of interferon-gamma (P = 0.005) and decreased interleukin-10 production (P = 0.03). These deficiencies may reflect a disturbance in the balance of the CD4(+)CD25(+) population, because in patients with type 1 diabetes, a higher proportion of these cells coexpress the early activation marker CD69 (P = 0.007) and intracellular CTLA-4 (P = 0.01). These data demonstrate deficiency in function of the CD4(+)CD25(+) Treg population that may influence the pathogenesis of type 1 diabetes.
Toward Automated Oligosaccharide Synthesis (2011)

Carbohydrates have been shown to play important roles in biological processes. The pace of development in carbohydrate research is, however, relatively slow due to the problems associated with the complexity of carbohydrate structures and the lack of general synthetic methods and tools available for the study of this class of biomolecules. Recent advances in synthesis have demonstrated that many of these problems can be circumvented. In this Review, we describe the methods developed to tackle the problems of carbohydrate-mediated biological processes, with particular focus on the issue related to the development of the automated synthesis of oligosaccharides. Further applications of carbohydrate microarrays and vaccines to human diseases are also highlighted.
12-month follow-up of fluoxetine and cognitive behavioral therapy for binge eating disorder. (2012)

This represents the first report from any randomized placebo-controlled trial for BED that has reported follow-up data after completing a course of medication-only treatment. CBT + placebo was superior to fluoxetine-only, and adding fluoxetine to CBT did not enhance findings compared to adding placebo to CBT. The findings document the longer term effectiveness of CBT, but not fluoxetine, through 12 months after treatment completion.
Zeptosens' protein microarrays: A novel high performance microarray platform for low abundance protein analysis (2002)

Protein microarrays are considered an enabling technology, which will significantly expand the scope of current protein expression and protein interaction analysis. Current technologies, such as two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry, allowing the identification of biologically relevant proteins, have a high resolving power, but also considerable limitations. As was demonstrated by Gygi et al. (Proc. Nat. Acad. Sci. USA 2000,97, 9390-9395), most spots in 2-DE, observed from whole cell extracts, are from high abundance proteins, whereas low abundance proteins, such as signaling molecules or kinases, are only poorly represented. Protein microarrays are expected to significantly expedite the discovery of new markers and targets of pharmaceutical interest, and to have the potential for high-throughput applications. Key factors to reach this goal are: high read-out sensitivity for quantification also of low abundance proteins, functional analysis of proteins, short assay analysis times, ease of handling and the ability to integrate a variety of different targets and new assays. Zeptosens has developed a revolutionary new bioanalytical system based on the proprietary planar waveguide technology which allows us to perform multiplexed, quantitative biomolecular interaction analysis with highest sensitivity in a microarray format upon utilizing the specific advantages of the evanescent field fluorescence detection. The analytical system, comprising an ultrasensitive fluorescence reader and microarray chips with integrated microfluidics, enables the user to generate a multitude of high fidelity data in applications such as protein expression profiling or investigating protein-protein interactions. In this paper, the important factors for developing high performance protein microarray systems, especially for targeting low abundant messengers of relevant biological information, will be discussed and the performance of the system will be demonstrated in experimental examples.
Use of hyaluronic acid fillers for the treatment of the aging face (2007)

Hyaluronic acid fillers have become popular soft tissue filler augmentation agents over the past several years. They have helped revolutionize the filler market with a number of new products available for use for our patients. The purpose of this manuscript is to review the characteristics of the HA fillers and to review each of the current products currently available for use in the US.
Maintenance procedures for the implant patient. (1991)

The purpose of this article is to provide the dental staff, the hygienist and or the assistant with a basic understanding of the most current update on the comprehensive clinical examination, documentation and instrumentation of the dental implant and its components. Although the primary issue in implant success is proper occlusal loading, prosthetic design and the distribution of the occlusal forces, one must also consider the importance of the maintenance of the implant environment. Understanding the implant environment and the rationale for impeccable bacterial control along with the principles for assessing the prosthesis and surrounding tissues may provide important information regarding the longterm success of the implant environment. The trained dental hygienist and or auxilliary can be instrumental in maximising the compliance, motivation and education of the patient as it relates to maintenance of the dental implant.
Triplex-induced Recombination in Human Cell-free Extracts (2001)

Triple helix-forming oligonucleotides (TFOs) can bind to polypurine/polypyrimidine regions in DNA in a sequence-specific manner. Triple helix formation has been shown to stimulate recombination in mammalian cells in both episomal and chromosomal targets containing direct repeat sequences. Bifunctional oligonucleotides consisting of a recombination donor domain tethered to a TFO domain were found to mediate site-specific recombination in an intracellular SV40 vector target. To elucidate the mechanism of triplex-induced recombination, we have examined the ability of intermolecular triplexes to provoke recombination within plasmid substrates in human cell-free extracts. An assay for reversion of a point mutation in the supFG1 gene in the plasmid pSupFG1/G144C was established in which recombination in the extracts was detected upon transformation into indicator bacteria. A bifunctional oligonucleotide containing a 30-nucleotide TFO domain linked to a 40-nucleotide donor domain was found to mediate gene correction in vitro at a frequency of 46 x 10(-)5, at least 20-fold above background and over 4-fold greater than the donor segment alone. Physical linkage of the TFO to the donor was unnecessary, as co-mixture of separate TFO and donor segments also yielded elevated gene correction frequencies. When the recombination and repair proteins HsRad51 and XPA were depleted from the extracts using specific antibodies, the triplex-induced recombination was diminished, but was either partially or completely restored upon supplementation with the purified HsRad51 or XPA proteins, respectively. These results establish that triplex-induced, intermolecular recombination between plasmid targets and short fragments of homologous DNA can be detected in human cell extracts and that this process is dependent on both XPA and HsRad51.
Isolation and Characterization of Salt-sensitive Mutants of the Moderate Halophile Halomonas elongata and Cloning of the Ectoine Synthesis Genes (1997)

The moderate halophile Halomonas elongata Deustche Sammlung für Mikroorganismen 3043 accumulated ectoine, hydroxyectoine, glutamate, and glutamine in response to osmotic stress (3 M NaCl). Two Tn1732-induced mutants, CHR62 and CHR63, that were severely affected in their salt tolerance were isolated. Mutant CHR62 could not grow above 0.75 M NaCl, and CHR63 did not grow above 1.5 M NaCl. These mutants did not synthesize ectoine but accumulated ectoine precursors, as shown by 13C NMR and mass spectroscopy. Mutant CHR62 accumulated low levels of diaminobutyric acid, and mutant CHR63 accumulated high concentrations of N-gamma-acetyldiaminobutyric acid. These results suggest that strain CHR62 could be defective in the gene for diaminobutyric acid acetyltransferase (ectB), and strain CHR63 could be defective in the gene for the ectoine synthase (ectC). Salt sensitivity of the mutants at 1.5-2.5 M NaCl could be partially corrected by cytoplasmic extracts of the wild-type strain, containing ectoine, and salt sensitivity of strain CHR62 could be partially repaired by the addition of extracts of strain CHR63, which contained N-gamma-acetyldiaminobutyric acid. This is the first evidence for the role of N-gamma-acetyldiaminobutyric acid as osmoprotectant. Finally, a cosmid from the H. elongata genomic library was isolated which complemented the Ect- phenotype of both mutants, indicating that it carried at least the genes ectB and ectC of the biosynthetic pathway of ectoine.
Application of an achromatic shearing phase sensor for the alignment of a segmented telescope (2002)

An achromatic shearing phase sensor is proposed as a phasing technique for the alignment of segmented telescopes. The sensor is based upon a shearing interferometer using two-wavelength interferometry methods. The two beams are created with a diffraction grating. The diffracted orders are re-imaged such that the pupil plane is focused onto a CCD array with a shear displacing the two orders. The amount of shear is equal to the size of the re-imaged segments. The sensor was measured to have a capture range of at least 5 μm, and an accuracy of 0.3 μm or better. The repeatability was 0.1 vm. The sensor is very sensitive to field dependent aberrations in its optical design but the resulting errors can be calibrated. The sensor is an improvement over similar technologies because it can measure and compensate for segment aberrations with tilt and piston adjustments. The sensor is compatible with many mature interferometry techniques and can be used with extended and broadband sources.
Fuzzy array dataflow analysis (1995)

Exact array dataflow analysis can be achieved in the general case if the only control structures are do-loops and structural ifs, and if loop counter bounds and array subscripts are affine expressions of englobing loop counters and possibly some integer constants. In this paper, we begin the study of dataflow analysis of dynamic control programs, where arbitrary ifs and whiles are allowed. In the general case, this dataflow analysis can only be fuzzy.
Duodenal Infusion of Donor Feces for Recurrent Clostridium difficile (2013)

The infusion of donor feces was significantly more effective for the treatment of recurrent C. difficile infection than the use of vancomycin. (Funded by the Netherlands Organization for Health Research and Development and the Netherlands Organization for Scientific Research; Netherlands Trial Register number, NTR1177.).
Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpT (2004)

Escherichia coli OmpP is an F episome-encoded outer membrane protease that exhibits 71% amino acid sequence identity with OmpT. These two enzymes cleave substrate polypeptides primarily between pairs of basic amino acids. We found that, like OmpT, purified OmpP is active only in the presence of lipopolysaccharide. With optimal peptide substrates, OmpP exhibits high catalytic efficiency (k(cat)/K(m) = 3.0 x 10(6) M(-1)s(-1)). Analysis of the extended amino acid specificity of OmpP by substrate phage revealed that both Arg and Lys are strongly preferred at the P1 and P1' sites of the enzyme. In addition, Thr, Arg, or Ala is preferred at P2; Leu, Ala, or Glu is preferred at P4; and Arg is preferred at P3'. Notable differences in OmpP and OmpT specificities include the greater ability of OmpP to accept Lys at the P1 or P1', site as well as the prominence of Ser at P3 in OmpP substrates. Likewise, the OmpP P1 site could better accommodate Ser; as a result, OmpP was able to cleave a peptide substrate between Ser-Arg about 120 times more efficiently than was OmpT. Interestingly, OmpP and OmpT cleave peptides with three consecutive Arg residues at different sites, a difference in specificity that might be important in the inactivation of cationic antimicrobial peptides. Accordingly, we show that the presence of an F' episome results in increased resistance to the antimicrobial peptide protamine both in ompT mutants and in wild-type E. coli cells.
Chondroitin sulfate proteoglycans in the developing central nervous system. II. Immunocytochemical localization of neurocan and phosphacan (1996)

Using immunocytochemistry, we have compared the distribution of neurocan and phosphacan in the developing central nervous system. At embryonic day 13 (E13), phosphacan surrounds the radially oriented neuroepithelial cells of the telencephalon, whereas neurocan staining of brain parenchyma is very weak. By E16-19, strong staining of both neurocan and phosphacan is seen in the marginal zone and subplate of the neocortex, and phosphacan is present in the ventricular zone and also has a diffuse distribution in other brain areas. Phosphacan is also widely distributed in embryonic spinal cord, where it is strongly expressed throughout the gray and white matter, in the dorsal and ventral nerve roots, and in the roof plate at E13, when neurocan immunoreactivity is seen only in the mesenchyme of the future spinal canal. Neurocan first begins to appear in the spinal cord at E16-19, in the region of ventral motor neurons. In early postnatal and adult cerebellum, neurocan immunoreactivity is seen in the prospective white matter and in the granule cell, Purkinje cell, and molecular layers, whereas phosphacan immunoreactivity is associated with Bergmann glial fibers in the molecular layer and their cell bodies (the Golgi epithelial cells) below the Purkinje cells. These immunocytochemical results demonstrate that the expression of neurocan and phosphacan follow different developmental time courses not only in postnatal brain (as previously demonstrated by radioimmunoassay) but also in the embryonic central nervous system. The specific localization and different temporal expression patterns of these two proteoglycans are consistent with other evidence indicating that they have overlapping or complementary roles in axon guidance, cell interactions, and neurite outgrowth during nervous tissue histogenesis.
Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope. (1994)

The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope present on more than 40 proteins of M-phase eukaryotic cells. We have developed a technique for cloning cDNAs encoding MPM2-reactive phosphoproteins from bacteriophage lambda expression libraries. Proteins from phage plaques were absorbed to nitrocellulose filters, phosphorylated by M-phase kinases, and screened for MPM2 binding. Partial-length cDNAs encoding two MPM2-reactive proteins termed MPM2-reactive phosphoproteins 1 and 2 (MPP1 and MPP2) were isolated. The deduced MPP1 and MPP2 amino acid sequences are not closely related to any previously described proteins. To determine which amino acid stretches contained the MPM2 epitope, sequences from a 15 amino acid peptide expression library were selected for binding to MPM2 after phosphorylation by M-phase kinases. A string of five amino acids was similar among all selected peptides, and the sequence reflecting the most frequent amino acid at each position was Leu-Thr-Pro-Leu-Lys (LTPLK). MPP1 and MPP2 proteins, respectively, contained five and nine sites closely related to LTPLK, including two that were common to both proteins, (F/T)TPLQ and SSP(I/S)D. Peptides containing LTPLK and FTPLQ were strongly phosphorylated by M-phase, but not interphase, cytosolic kinases, and the phosphorylated peptides were bound by MPM2. Thus, we have identified M-phase-specific phosphorylation sites bound by MPM2 and two putative M-phase phosphoproteins containing these sites.
Advances in Microsurgery. (1991)

The beginnings of microsurgery date back to the early part of the century. In 1906, the first description of small vessel anastomosis was presented to the Johns Hopkins Medical Society by Alexis Carrel, who eventually won the Nobel Prize for his work in 1912. The next major advance, reported by Jacobson and Suarez in 1960, was the anastomosis of 1mm vessels. This feat was a pivotal accomplishment and soon became clinically feasible with the manufacture of fine needles (50μ to 100μ) and sutures measuring between 15μ and 20μ, or approximately three times the size of a human red blood cell. Two years later, Malt performed a landmark operation the first replantation of an upper extremity above the elbow and soon after, Susumi Tamai of Japan successfully reattached a completely amputated thumb. The first toe-to-thumb transplant was performed in the early 1970s, and, within a few years, free microvascular muscle and bone transplantation had been described. By the early 1980s, microsurgical procedures had undergone a dramatic evolution, seeing the development of an expansive array of microvascular alternatives to address a wide variety of clinical problems.
Mechanism of Immunosuppression in Males Following Trauma-Hemorrhage (1996)

Castration before soft-tissue trauma and hemorrhagic shock maintains normal immune function in male mice, but sham-castrated male mice show significant immunodepression. The maintenance of immune function by androgen deficiency does not seem to be related to changes in the release of corticosterone. We conclude that male sex steroids are involved in the immunodepression observed after trauma-hemorrhage. Thus, the use of testosterone-blocking agents following trauma-hemorrhage should prevent the depression of immune functions and decrease the susceptibility to sepsis under those conditions.