gebhart/patrepeval huggingface dataset:
A next-generation optical regional access network (2000)

We describe an optical regional access network which combines electronic IP routing with intelligent networking functionality of the optical WDM layer. The optical WDM layer provides such networking functions as network logical topology reconfiguration, optical flow switching to offload traffic and bypass IP routers, wavelength routing of signals, protection switching and restoration in the optical domain, and flexible network service provisioning by reconfigurable wavelength connectivity. We discuss key enabling technologies for the WDM layer and describe their limitations. The symbiosis of electronic and optical WDM networking functions also allows support for heterogeneous format traffic and will enable efficient gigabit-per-second user access in next-generation Internet networks.
Enzyme Nomenclature. Recommendations 1992 (1995)

This document contains further additions and amendments to Enzyme Nomenclature 1992 , published by Academic Press, Orlando, Florida. The previous Supplement was published in Eur. J. Biochem. 223 , 1–5 (1994). Comments and suggestions on enzyme classification and nomenclature may be sent to Prof. K. F. Tipton, Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland.
Effects of Polyethylene Glycol on DNA Adsorption and Hybridization on Gold Nanoparticles and Graphene Oxide (2012)

Understanding the interface between DNA and nanomaterials is crucial for rational design and optimization of biosensors and drug delivery systems. For detection and delivery into cells, where high concentrations of cellular proteins are present, another layer of complexity is added. In this context, we employ polyethylene glycol (PEG) as a model polymer to mimic the excluded volume effect of cellular proteins and to test its effects on DNA adsorption and hybridization on gold nanoparticles (AuNPs) and graphene oxide (GO), both of which show great promise for designing intracellular biosensors and drug delivery systems. We show that PEG 20000 (e.g., 4%) accelerates DNA hybridization to DNA-functionalized AuNPs by 50-100%, but this enhanced hybridization kinetics has not been observed with free DNA. Therefore, this rate enhancement is attributed to the surface blocking effect by PEG instead of the macromolecular crowding effect. On the other hand, DNA adsorption on citrate-capped AuNP surfaces is impeded even in the presence of a trace level (i.e., parts per billion) of PEG, confirming PEG competes with DNA for surface binding sites. Additional insights have been obtained by studying the adsorption of a thiolated DNA and a peptide nucleic acid. In these cases, the steric effects of PEG to impede adsorption are observed. Similar observations have also been made with GO. Therefore, PEG may be used as an effective blocking agent for both hydrophilic AuNP and for GO that also contains hydrophobic domains.
Leptin Impairs Metabolic Actions of Insulin in Isolated Rat Adipocytes (1997)

Leptin is an adipocyte hormone involved in the regulation of energy homeostasis. Generally accepted biological effects of leptin are inhibition of food intake and stimulation of metabolic rate in ob/ob mice that are defective in the leptin gene. In contrast to these centrally mediated effects of leptin, we are reporting here on leptin effects on isolated rat adipocytes. Leptin impairs several metabolic actions of insulin, i.e. stimulation of glucose transport, glycogen synthase, lipogenesis, inhibition of isoproterenol-induced lipolysis, and protein kinase A activation, as well as stimulation of protein synthesis. Insulin effects were reduced by leptin (2 nM) with a half-life of about 8 h. At low leptin concentrations (<1 nM), the insulin sensitivity was reduced leading to a shift to the right in the dose-response curve. At higher concentrations the responsiveness was diminished, resulting in nearly complete inhibition of insulin effects at >30 nM leptin. The IC50 value of leptin was 3.1 +/- 1 nM after 15 h of preincubation of adipocytes in primary culture. The natural splice variant des-Gln49-leptin exhibited a significantly lower potency. Adipocytes regained full insulin sensitivity within a few hours after leptin removal. The stimulation of glucose transport by vanadate was not affected by leptin. These data show specific and potent impairment of insulin action by leptin in the physiological concentration range of both leptin and insulin, which may be related to the pathophysiology of insulin resistance in both non-insulin-dependent diabetes mellitus and obesity.
Cytotoxic T Lymphocyte Responses to E6 and E7 Proteins of Human Papillomavirus Type 16: Relationship to Cervical Intraepithelial Neoplasia (1997)

Cytotoxic T lymphocyte (CTL) responses to the human papillomavirus (HPV) type 16 E6 and E7 proteins were measured in 20 women with known HPV and cervical disease status. CTL assays were performed after stimulation with E6 or E7 fusion proteins using autologous B lymphoblastoid cells infected with vaccinia viruses expressing E6 or E7. CTL responses to E6 and E7 were detected in 6 (75%) of 8 and 5 (56%) of 9 HPV-16-positive women without cervical intraepithelial neoplasia (CIN), respectively. Responses to E6 or E7 were each detected in only 2 (29%) of 7 HPV-16-positive women with CIN. Responses to both antigens were found in 63% of women without CIN and 14% of those with CIN. CTL responses to E6 or E7 are more commonly detectable in HPV-16-positive women without CIN than in HPV-16-positive women with CIN, suggesting that CTL response may play a role in disease protection.
Studies on colony formation in vitro by mouse bone marrow cells. II. Action of colony stimulating factor (1970)

Abstract An analysis was made of some of the processes involved in the stimulation by colony stimulating factor (CSF) of cluster and colony formation by mouse bone marrow cells in agar cultures in vitro . Colony formation was shown to be related to the concentration and not the total amount of CSF. The concentration of CSF determined the rate of new cluster initiation in cultures and the rate of growth of individual clusters. Colony growth depleted the medium of CSF suggesting that colony cells may utilise CSF during proliferation. Bone marrow cells incubated in agar in the absence of CSF rapidly died or lost their capacity to proliferate and form clusters or colonies. CSF appears (a) to be necessary for survival of cluster‐and colony‐forming cells or for survival of their proliferative potential, (b) to shorten the lag period before individual cells commence proliferation and (c) to increase the growth rate of individual clusters and colonies.
Lysine-specific demethylase 1 (LSD1) in hematopoietic and lymphoid neoplasms (2014)

To the editor:

Recently, inhibition of lysine-specific demethylase 1 (LSD1) has gained attention as a potential novel treatment in acute myeloid leukemia (AML).[1][1][⇓][2]-[3][3] However, expression in other hematologic neoplasms has not been examined. LSD1 is a central epigenetic regulator of
Mammary tumorigenesis and metastasis in transgenic mice. (1994)

The transgenic mouse has emerged as an important model system to assess the transforming potential of oncogenes in the mammary epithelium. Mammary gland-specific expression of oncogenes in transgenic mice has resulted in the induction of a variety of phenotypes ranging from benign epithelial hyperplasias to metastatic mammary tumors. The induction of tumors in most of these transgenic models is a multi-step process where transgene expression, although required, is not sufficient for conversion of the primary mammary epithelial cell to the transformed phenotype. While the identity of many of these collaborating genetic events is obscure, several approaches have been applied with might shed light on their nature. In a few exceptional transgenic strains, tumor progression can occur very rapidly suggesting that, if additional genetic events are required, they occur very frequently. Recent genetic and biochemical characterization of these strains offers insight into the molecular mechanisms that may underlie the complex phenotypic features exhibited by these transgenic strains.
Crystal Design of Barium Sulfate using Double-Hydrophilic Block Copolymers (2000)

Peach, peanut, fiber, and flower (see picture) crystal morphologies are achieved from the precipitation of simple minerals in the presence of specifically adsorbing polymers. These crystal design effects are illustrated using BaSO(4) and double-hydrophilic block copolymers, the latter featuring carboxylate, sulfonate, phosphonate, and aspartic acid groups.
The Inflammasome NLRs in Immunity, Inflammation, and Associated Diseases (2011)

Inflammasome activation leads to caspase-1 activation, which causes the maturation and secretion of pro-IL-1β and pro-IL-18 among other substrates. A subgroup of the NLR (nucleotide-binding domain, leucine-rich repeat containing) proteins are key mediators of the inflammasome. Studies of gene-deficient mice and cells have implicated NLR inflammasomes in a host of responses to a wide range of microbial pathogens, inflammatory diseases, cancer, and metabolic and autoimmune disorders. Determining exactly how the inflammasome is activated in these diseases and disease models remains a challenge. This review presents and integrates recent progress in the field.
Diastereoselection in the Formation of Spirocyclic Oxindoles by the Intramolecular Heck Reaction (2006)

Diastereoselective double Heck cyclizations of cyclohexene diamides 1 and 3 form contiguous quaternary stereocenters, with diastereoselection being controlled by the trans-diol protecting group. In this, the first in a series of two papers, the origin of diastereoselection in the first ring-closure step of these reactions is examined. Nine simplified analogues of 1 and 3 were synthesized and cyclized to discern what structural features are required to realize high diastereoselection in the first intramolecular Heck reaction. These studies show that high stereoselection (>20:1) does not arise from a single structural feature: it is seen only in substrates that contain both a trans-acetonide and a tertiary amide substituent at C2. Two subtle factors appear to be involved: (1) Avoidance of eclipsing interactions between the forming C-C bond and the pseudoaxial hydrogen atom at C6 and between the pseudoequatorial hydrogen atom at C6 and the carbonyl carbon of the forming spirooxindole. (2) The vinylic amide substituent that is not involved in the insertion event preferentially adopts a perpendicular conformation, placing the sterically bulky NR(2) over the alkene pi bond. syn-Pentane-like interactions between this substituent and the C3 of the cyclohexene are avoided in the favored insertion topography. These two effects, when combined, produce a highly diastereoselective process.
Elucidating Distinct Roles for NF1 in Melanomagenesis (2012)

BRAF mutations play a well-established role in melanomagenesis; however, without additional genetic alterations, tumor development is restricted by oncogene-induced senescence (OIS). Here, we show that mutations in the NF1 tumor suppressor gene cooperate with BRAF mutations in melanomagenesis by preventing OIS. In a genetically engineered mouse model, Nf1 mutations suppress Braf-induced senescence, promote melanocyte hyperproliferation, and enhance melanoma development. Nf1 mutations function by deregulating both phosphoinositide 3-kinase and extracellular signal-regulated kinase pathways. As such, Nf1/Braf-mutant tumors are resistant to BRAF inhibitors but are sensitive to combined inhibition of mitogen-activated protein/extracellular signal-regulated kinase kinase and mTOR. Importantly, NF1 is mutated or suppressed in human melanomas that harbor concurrent BRAF mutations, NF1 ablation decreases the sensitivity of melanoma cell lines to BRAF inhibitors, and NF1 is lost in tumors from patients following treatment with these agents. Collectively, these studies provide mechanistic insight into how NF1 cooperates with BRAF mutations in melanoma and show that NF1/neurofibromin inactivation may have an impact on responses to targeted therapies.
Multivesicular Body Morphogenesis (2011)

Multivesicular bodies (MVBs) are unique organelles in the endocytic pathway that contain vesicles in their lumen. Sorting and incorporation of material into such vesicles is a critical cellular process that has been intensely studied following discovery of the ESCRT (endosomal sorting complex required for transport) machinery just more than a decade ago. In this review, we summarize current understanding of the cellular functions of MVBs and how the ESCRT machinery contributes to MVB morphogenesis. We also highlight the importance of MVBs and ESCRTs in human health. We identify critical areas in which further mechanistic and spatiotemporal studies in living cells will advance this exciting area of research.
The diagnostic value of amniotic fluid Gram stain examination and limulus amebocyte lysate assay in patients with preterm birth (1995)

The purpose of this study was to determine the value of Gram stain examination and Limulus amebocyte lysate (LAL) test in the detection of intraamniotic infection. Ninety women with preterm labor and intact membranes (n = 55) or preterm premature rupture of membranes (PROM) (n = 35) who delivered prematurely were included in the study. Amniotic fluid was cultured for aerobic and anaerobic bacteria as well as for mycoplasmas. Amniotic fluid analysis included Gram stain examination and limulus amebocyte lysate tests. The prevalence of positive amniotic culture was 32.2% (29/90) and the most common isolate was Ureaplasma urealyticum. Patients with preterm PROM group had a higher rate of infection than those with preterm labor and intact membranes 57.1% (20/35) vs. 16.4% (9/55), respectively (p = 0.0001). We found a lower gestational age at delivery and lower mean birth weight in neonates born to mothers with a positive amniotic fluid culture than those with negative amniotic fluid culture. The combined use of Gram stain examination and LAL test had a sensitivity and specificity of 51.7% (15/29) and 95.1% (58/61) respectively for the detection of positive amniotic fluid culture. We conclude that Gram stain examination and LAL test are rapid, simple and specific tests that can be used to detect microbial invasion of the amniotic cavity except in patients with mycoplasmas infections.
In Vivo Measurement of Dimensions of Veins with Implications Regarding Control of Venous Return (1969)

Control of the capacity of the venous system has been postulated as necessary to provide increased venous return to initiate an increase in cardiac output. In chronic animal experiments, we have studied the dimensions of the liver, spleen, and the superior vena cava as venous reservoirs in relation to fright, exercise, and shock. Two methods used were miniature mutual inductance coils and ultrasonic transit time technique; the former is a relatively simple and reliable, but nonquantifiable method. The sonar method is quantifiable, but to date has been unsatisfactory for narrow ranges. The spleen in the dog is a superb volume reservoir, but the liver is not. The superior vena cava, in at least some circumstances, demonstrates active constriction. With the onset of exercise, flow increased in the cava before either an increase in heart rate or an increase in stroke volume. With hemorrhagic shock, pooling of blood in the mesenteric veins was sometimes found, but there was no evidence of failure of the neural mechanisms of venoconstriction, evidenced by a marked, agonal contraction. With endotoxin shock, no portal pooling was observed; earlier reports of pooling based on weight changes were probably due to extravasated plasma secondary to endothelial damage and marked portal venoconstriction. Venous pooling in the legs in man in shock can be prevented by elevation of the legs without the disadvantages of the head-down (Trendelenburg) position.
Cubic and Hexagonal Liquid Crystals as Drug Delivery Systems (2014)

Lipids have been widely used as main constituents in various drug delivery systems, such as liposomes, solid lipid nanoparticles, nanostructured lipid carriers, and lipid-based lyotropic liquid crystals. Among them, lipid-based lyotropic liquid crystals have highly ordered, thermodynamically stable internal nanostructure, thereby offering the potential as a sustained drug release matrix. The intricate nanostructures of the cubic phase and hexagonal phase have been shown to provide diffusion controlled release of active pharmaceutical ingredients with a wide range of molecular weights and polarities. In addition, the biodegradable and biocompatible nature of lipids demonstrates the minimum toxicity and thus they are used for various routes of administration. Therefore, the research on lipid-based lyotropic liquid crystalline phases has attracted a lot of attention in recent years. This review will provide an overview of the lipids used to prepare cubic phase and hexagonal phase at physiological temperature, as well as the influencing factors on the phase transition of liquid crystals. In particular, the most current research progresses on cubic and hexagonal phases as drug delivery systems will be discussed.
Synthesis of Allenes from Allylic Alcohol Derivatives Bearing a Bromine Atom Using a Palladium(0)/Diethylzinc System (2002)

A general and efficient synthesis of allenes using a palladium(0)/diethylzinc system is described. Treatment of mesylates or trichloroacetates of (E)- or (Z)-2-bromoalk-2-en-1-ols with diethylzinc in the presence of a catalytic amount of palladium(0) affords allenes bearing an aminoalkyl, alkyl, or aryl substituent(s) in good to high yields. No transfer of chirality from the stereogenic center carrying the mesyloxy group to the allene was observed.
Continuous multi-views tracking using tensor voting (2003)

The paper presents a new approach for continuous tracking of moving objects observed by multiple fixed cameras. The continuous tracking of moving objects in each view is realized using a tensor voting based approach. We infer objects' trajectories by performing a perceptual grouping in 2D+t using tensor voting. Also, a multi-scale approach to bridge gaps in object trajectories is presented The trajectories obtained from the multiple cameras are registered in space and time, allowing a synchronization of the video streams and a continuous tracking of objects across multiple views. We demonstrate the performance of the system on several real video surveillance sequences.
Visualization of Dynamic Trafficking of a Protein Kinase C βII/Green Fluorescent Protein Conjugate Reveals Differences in G Protein-coupled Receptor Activation and Desensitization (1998)

Protein kinase C (PKC) links various extracellular signals to intracellular responses and is activated by diverse intracellular factors including diacylglycerol, Ca2+, and arachidonic acid. In this study, using a fully functional green fluorescent protein conjugated PKCbetaII (GFP-PKCbetaII), we demonstrate a novel approach to study the dynamic redistribution of PKC in live cells in response to G protein-coupled receptor activation. Agonist-induced PKC translocation was rapid, transient, and selectively mediated by the activation of Gqalpha- but not Gsalpha- or Gialpha-coupled receptors. Interestingly, although the stimuli were continuously present, only one brief peak of PKC membrane translocation was observed, consistent with rapid desensitization of the signaling pathway. Moreover, when GFP-PKCbetaII was used to examine cross-talk between two Gqalpha-coupled receptors, angiotensin II type 1A receptor (AT1AR) and endothelin A receptor (ETAR), activation of ETARs resulted in a subsequent loss of AT1AR responsiveness, whereas stimulation of AT1ARs did not cause desensitization of the ETAR signaling. The development of GFP-PKCbetaII has allowed not only the real time visualization of the dynamic PKC trafficking in live cells in response to physiological stimuli but has also provided a direct and sensitive means in the assessment of activation and desensitization of receptors implicated in the phospholipase C signaling pathway.
Production and Purification of d -Aminoacylase from Alcaligenes denitrificans and Taxonomic Study of the Strain (1988)

A d-aminoacylase-producing microorganism, strain DA181, isolated from soil was identified as Alcaligenes denitrificans subsp. denitrificans. This strain produced about 29,300 units (micromoles of product formed per hour) of d-aminoacylase and 2,300 units of l-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1% N-acetyl-dl-leucine as the carbon source. The d-aminoacylase was purified 345-fold. The specific activity of the purified enzyme was 108,600 units per mg of protein when N-acetyl-d-methionine was used as a substrate. The apparent molecular weight was 58,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-Acetyl-d-methionine was the favored substrate, followed by N-acetyl-d-phenylalanine. This enzyme had a high stereospecificity, and its hydrolysis of N-acetyl-l-amino acids was almost negligible.
Study of a New Schizophrenomimetic Drug—Sernyl (1959)

<h3>Introduction</h3> The production of model psychoses has been employed as a technique for testing hypotheses relevant to the causes, correlates, and treatment of schizophrenia since 1921, when De Jong demonstrated experimental catatonia by giving bulbocapnine to animals.<sup>5</sup>This method of study has received considerable impetus with the discovery of the hallucinogenic properties of lysergic acid diethylamide (LSD-25)<sup>22</sup>and other psychotomimetic agents. Objections have since been raised to the drawing of premature analogies between the psychotic-like states produced by drugs and the psychopathology of schizophrenia. Ebaugh has pointed out that "the difference between these pseudopsychoses and schizophrenia is so striking that such experimentation seems to have only tenuous relevance to the problem of schizophrenia."<sup>7</sup>On the other hand, Wikler has asserted that "from the standpoint of the development of dynamic (causal) concepts about human behavior, it matters little whether or not model psychoses resemble schizophrenia. Drugs which modify
Real-time femtosecond probing of ‘‘transition states’’ in chemical reactions (1987)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Marcos Dantus, Mark J. Rosker, Ahmed H. Zewail; Real‐time femtosecond probing of ''transition states'' in chemical reactions. J. Chem. Phys. 15 August 1987; 87 (4): 2395–2397. https://doi.org/10.1063/1.453122 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioThe Journal of Chemical Physics Search Advanced Search |Citation Search
Evidence forp-Type Doping of InN (2006)

The first evidence of successful p-type doping of InN is presented. It is shown that InN:Mg films consist of a p-type bulk region with a thin n-type inversion layer at the surface that prevents electrical contact to the bulk. Capacitance-voltage measurements indicate a net concentration of ionized acceptors below the -type surface. Irradiation with 2 MeV He+ ions is used to convert the bulk of InN:Mg from p to n-type, at which point photoluminescence is recovered. The conversion is well explained by a model assuming two parallel conducting layers (the surface and the bulk) in the films.