gebhart/patrepeval huggingface dataset:
The Effect of Vitamin D on Falls: A Systematic Review and Meta-Analysis (2011)

Vitamin D combined with calcium reduces the risk of falls. The reduction in studies without calcium coadministration did not reach statistical significance. The majority of the evidence is derived from trials enrolling elderly women.
PROBABILISTIC PREDICTION OF RHYTHMIC CHARACTERISTICS IN MARKOV CHAIN-BASED MELODIC SEQUENCES (2013)

Markov chain models have been widely used for algorithmic composition and machine improvisation.In this paper, we introduce a probabilistic prediction model of rhythmic characteristics of Markov chain-based note sequences.For this purpose, we propose an algorithm to generate a revised Markov chain model and calculate the onset probabilities of notes at each onset position in one measure.As an application of this algorithm, we present an interactive improvisation system which uses a customized syncopation index as an input parameter and allows the user to control the level of syncopation and rhythmic tension in real-time.
Ultra-Low Temperature Deposition of Copper Seed Layers by PEALD (2010)

Two novel fluorine-free precursors have been developed and evaluated for use in ultra-low temperature copper deposition by PEALD. Manufacturable PEALD processes have been demonstrated, and processing conditions correlated to film composition, growth rate, resistivity and step coverage. Preliminary integration results indicate that both of these precursors have potential for use in advanced interconnect systems.
High-level Biosynthetic Substitution of Methionine in Proteins by its Analogs 2-Aminohexanoic Acid, Selenomethionine, Telluromethionine and Ethionine in Escherichia coli (1995)

International audience
Analysis of glycoprotein-derived glycopeptides (2000)

Glycosylation of proteins represents one of the most important post-(co-)translational events in view of the ubiquity of the phenomenon. In most cases, the covalently linked glycans are involved in the functioning of these biomolecules in biological systems. Detailed information on the carbohydrate moieties including monosaccharide composition, anomeric configurations, type of glycosidic linkages and attachment sites at the protein is indispensable in describing the ultimate structure of a specific glycoprotein. This chapter presents a general strategy for the structural characterization of glycoproteins/glycopeptides focussed on the glycan part. Some of the techniques commonly used, like enzyme treatments, separation methods, chemical analyses, mass spectrometry and nuclear magnetic resonance spectroscopy are briefly reviewed.
Herpes Simplex Virus 1 Amplicon Vector-Mediated siRNA Targeting Epidermal Growth Factor Receptor Inhibits Growth of Human Glioma Cells in Vivo (2005)

In primary glioblastomas and other tumor types, the epidermal growth factor receptor (EGFR) is frequently observed with alterations, such as amplification, structural rearrangements, or overexpression of the gene, suggesting an important role in glial tumorigenesis and progression. In this study, we investigated whether posttranscriptional gene silencing by vector-mediated RNAi to inhibit EGFR expression can reduce the growth of cultured human gli36 glioma cells. To "knock down" EGFR expression, we have created HSV-1-based amplicons that contain the RNA polymerase III-dependent H1 promoter to express double-stranded hairpin RNA directed against EGFR at two different locations (pHSVsiEGFR I and pHSVsiEGFR II). We demonstrate that both pHSVsiEGFR I and pHSVsiEGFR II mediated knock-down of transiently transfected full-length EGFR or endogenous EGFR in a dose-dependent manner. The knock-down of EGFR resulted in the growth inhibition of human glioblastoma (gli36-luc) cells both in culture and in athymic mice in vivo. Cell cycle analysis and annexin V staining revealed that siRNA-mediated suppression of EGFR induced apoptosis. Overall HSV-1 amplicons can mediate efficient and specific posttranscriptional gene silencing.
A Hybrid Radar-Camera Sensing System With Phase Compensation for Random Body Movement Cancellation in Doppler Vital Sign Detection (2013)

This paper presents a Doppler radar vital sign detection system with random body movement cancellation (RBMC) technique based on adaptive phase compensation. An ordinary camera was integrated with the system to measure the subject's random body movement (RBM) that is fed back as phase information to the radar system for RBMC. The linearity of the radar system, which is strictly related to the circuit saturation problem in noncontact vital sign detection, has been thoroughly analyzed and discussed. It shows that larger body movement does not necessarily mean larger radar baseband output. High gain configuration at baseband is required for acceptable SNR in noncontact vital sign detection. The phase compensation at radar RF front-end helps to relieve the high-gain baseband from potential saturation in the presence of large body movement. A simple video processing algorithm was presented to extract the RBM without using any marker. Both theoretical analysis and simulation have been carried out to validate the linearity analysis and the proposed RBMC technique. Two experiments were carried out in the lab environment. One is the phase compensation at RF front end to extract a phantom motion in the presence of another large shaker motion, and the other one is to measure the subject person breathing normally but randomly moving his body back and forth. The experimental results show that the proposed radar system is effective to relieve the linearity burden of the baseband circuit and help compensate the RBM.
Regulation of Ion Channel Expression (2004)

A potentially important mechanism controlling ion channel expression is homeostatic regulation, which can act to maintain a stable electrophysiological phenotype in cardiac myocytes as well as to provide plasticity in response to genetic, pathological, or pharmacological insults. The capabilities and limitations of the homeostatic regulatory mechanisms that contribute to the control of cardiac ion channel expression are the primary topic of this review.
Specific inhibition of T‐cell adhesion to extracellular matrix and proinflammatory cytokine secretion by human recombinant galectin‐1 (1999)

The migration of immune cells through the extracellular matrix (ECM) towards inflammatory sites is co-ordinated by receptors recognizing ECM glycoproteins, chemokines and proinflammatory cytokines. In this context, galectins are secreted to the extracellular milieu, where they recognize poly-N-acetyllactosamine chains on major ECM glycoproteins, such as fibronectin and laminin. We investigated the possibility that galectin-1 could modulate the adhesion of human T cells to ECM and ECM components. T cells were purified from human blood, activated with interleukin-2 (IL-2), labelled, and incubated further with intact immobilized ECM and ECM glycoproteins in the presence of increasing concentrations of human recombinant galectin-1, or its more stable, related, C2-S molecule obtained by site-directed mutagenesis. The presence of galectin-1 was shown to inhibit T-cell adhesion to intact ECM, laminin and fibronectin, and to a lesser extent to collagen type IV, in a dose-dependent manner. This effect was specifically blocked by anti-galectin-1 antibody and was dependent on the lectin's carbohydrate-binding properties. The inhibition of T-cell adhesion by galectin-1 correlates with the ability of this molecule to block the re-organization of the activated cell's actin cytoskeleton. Furthermore, tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) production was markedly reduced when IL-2-activated T cells were incubated with galectin-1 or its mutant. This effect was prevented by beta-galactoside-related sugars. The present study reveals an alternative inhibitory mechanism for explaining the suppressive properties of the galectin-1 subfamily on inflammatory and autoimmune processes.
Common sequence on distinct Vβ genes defines a protective idiotope in experimental encephalomyelitis (1992)

Synthetic TCR peptides expressed by encephalitogenic T cells can induce both cellular and humoral responses that protect against experimental encephalomyelitis. In the Lewis rat, encephalitogenic T cells predominantly express V beta 8.2, and a peptide in the CDR2 region representing residues 39-59 could both protect against and treat experimental autoimmune encephalitis (EAE). Similarly, the homologous and cross reactive 39-59 peptide from V beta 8.6 expressed by an EAE-protective clone also had protective and therapeutic activity against EAE. The consensus sequence between the V beta 8.2 and V beta 8.6 peptides, which included residues 44-54, was postulated to contain the protective idiotope. In this report, we demonstrate that this peptide, designated V beta 8-44-54, has comparable activity to the longer peptides for treating both active and passive EAE. Similar to the longer V beta 8.2-39-59 peptide, the V beta 8-44-54 peptide stimulates protective TCR peptide-specific CD4+, CD8dim T cells restricted by MHC I. We also report for the first time the recovery of V beta 8-44-54 reactive T cells that express a variety of V beta genes in their T-cell receptor (TCR), including V beta 4, 8, 10, 12, 15, 17, 19, and 20. Taken together, these data establish that the V beta 8-44-54 sequence constitutes an important autoregulatory idiotope in EAE.(ABSTRACT TRUNCATED AT 250 WORDS)
The epidemiology of co-occurring addictive and mental disorders: Implications for prevention and service utilization. (1996)

Peer Reviewed
Programmed death-1 ligands-transfected dendritic cells loaded with glutamic acid decarboxylase 65 (GAD65) inhibit both the alloresponse and the GAD65-reactive lymphocyte response (2007)

Type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, such as glutamic acid decarboxylase 65 (GAD65), for which islet transplantation is a promising therapy. Therefore, the generation of tolerance aiming at both alloantigen and GAD65 will help therapeutic intervention greatly in T1D. In this study, we tested the effect of programmed death-1 ligands (PD-L1)-transfected dendritic cells (DC) loaded with GAD65 on the alloresponse and GAD65-reactive lymphocyte response. The DC2.4 cell line was transfected with PD-L1 and co-cultured with GAD65. BALB-c mice were primed, respectively, by intraperitoneal injection with GAD65, PD-L1-transfected- or non-transfected DC (PD-L1/DC or DC), and PD-L1-transfected- or non-transfected DC loaded with GAD65 (PD-L1/DC/GAD65 or DC/GAD65). Splenocytes of treated mice were isolated and restimulated in vitro with GAD65 or the various DC populations above being used as stimulators, respectively. In the mixed lymphocyte reaction, DC/GAD65 were able to stimulate both allogeneic and GAD65-reactive lymphocytes. However, PD-L1/DC/GAD65 were poorer than DC/GAD65 at activating the GAD65-reactive lymphocyte response. Further, although PD-L1/DC could inhibit the alloresponse, PD-L1/DC/GAD65 were more effective at down-regulating the GAD65-reactive lymphocyte response. More importantly, PD-L1/DC/GAD65-primed lymphocytes exhibited the weakest proliferation when again restimulated in vitro by PD-L1/DC/GAD65. Additionally, PD-L1/DC/GAD65 down-regulated interferon-gamma and up-regulated interleukin-10 production by activated lymphocytes. Therefore, combined stimulation in vivo and in vitro by PD-L1/DC/GAD65 could inhibit both the alloresponse and the GAD65-reactive lymphocyte response, which may contribute to controlling diabetes and islet transplant rejection.
Variability of In Vivo Recovery of Factor IX after Infusion of Monoclonal Antibody Purified Factor IX Concentrates in Patients with Hemophilia B (1995)

Monoclonal antibody purified factor IX concentrate, Mononine (Armour Pharmaceutical Company, Kankakee, Illinois, USA), is a recently developed replacement factor concentrate for the treatment of patients with hemophilia B. The pharmacokinetic properties of monoclonal antibody purified factor IX concentrate (MAb Factor IX concentrate) have been evaluated in only small samples of patients, and little is known about those factors that might influenced in vivo recovery of factor IX after infusion is a larger patient population. In vivo recovery of factor IX was therefore evaluated for 80 different indications in 72 patients who received MAb Factor IX concentrate for the management of spontaneous or trauma-induced bleeding, or as prophylaxis with surgery. The average recovery after infusions for presurgical pharmacokinetic analysis (mean +/- standard deviation) was 1.28 +/- 0.56 U/dl rise per U/kg infused (range 0.41-2.80), and the average recovery after all infusions for treatment was 1.23 +/- 0.49 U/dl rise per U/kg infused (range - 0.35-2.92). Recovery values for multiple MAb Factor IX doses in a given patient were also variable; the average recovery was 1.22 +/- 0.53 U/dl rise per U/kg given, and standard deviations ranged from 0.03 to 1.26. Patient age, weight, and MAb Factor IX concentrate dose minimally but significantly influenced factor IX recovery. There was no significant effect of either race, history of previous thrombotic complications during treatment with other replacement factor concentrates, or bleeding state on recovery. All of the patients treated with this preparation experienced excellent hemostasis, and no thrombotic complications were observed.
An autonomic messaging middleware with stateful stream transformation (2004)

We report on the design and prototype implementation of the SMILE system. SMILE (Smart Middleware Light Ends) extends the Gryphon publish-subscribe system with stateful subscriptions and computing capabilities, with the goal of raising the abstraction level of messaging middleware programming closer to that of database programming. It allows subscriptions to relational views (which can involve aggregation, joins, and other transforms) derived from published event streams. The SMILE system has dual autonomic properties: it can be used to augment other system with monitoring capabilities and make them more autonomic; and the system itself has sophisticated capabilities for self-optimization, deployment and automated fault-tolerance.
p53 Represses CAAT Enhancer-binding Protein (C/EBP)-dependent Transcription of the Albumin Gene (1999)

p53 is a transcription factor that is activated by genotoxic stress and mediates cell cycle arrest and apoptosis. Here we demonstrate that infection of mouse liver with recombinant E1/E3-deleted adenovirus leads to p53 activation and simultaneously to the down-regulation of albumin gene expression. In vitro transcription assays indicate that transcriptional mechanisms mediated through the albumin promoter are responsible for reduced albumin mRNA levels during viral infection. Albumin expression is maintained in the liver by a combination of liver-enriched transcription factors such as CAAT enhancer-binding protein (C/EBP)alpha and C/EBPbeta. We show that p53 wild type and tumor-derived p53 mutations repress C/EBP-mediated transactivation of the albumin promoter. The binding of C/EBPalpha or -beta to its cognate sequence in the albumin promoter is not inhibited by p53 expression. Deletion analysis and domain swapping experiments show that repression of C/EBPbeta-mediated transactivation is dependent on the N-terminal domain of p53 and the transactivation domain, leucine zipper domain, and the inhibitory domain II (amino acids 163-191) of C/EBPbeta. Our results provide a molecular explanation for the p53-mediated down-regulation of liver-specific gene expression after viral infection. Additionally, as overexpression of p53 mutants is frequently found in undifferentiated hepatocellular carcinomas, the same mechanisms may contribute to the lack of liver-specific gene transcription in these tumors.
Dynamics of Colloids in Single Solid-State Nanopores (2011)

We use solid-state nanopores to study the dynamics of single electrically charged colloids through nanopores as a function of applied voltage. We show that the presence of a single colloid inside of the pore changes the pore resistance, in agreement with theory. The normalized ionic current blockade increases with the applied voltage and remains constant when the electrical force increases even more. We observe short and long events of current blockades. Their durations are associated, respectively, with low and high current variation. The ratio of long events increases with the electrical force. The events frequency increases exponentially as a function of applied voltage and saturates at high voltage. The dwelling time decreases exponentially at low and medium voltages when the electrical force increases. At large voltages, this time decreases inversely proportionally to the applied voltage. The long events are associated with translocation events. We show that the dynamics of colloids through the nanopore is governed mainly by two mechanisms, by the free-energy barrier at relatively low and medium voltages and by the electrophoresis mechanism at high voltage.
Bending bilayer strips built from polyaniline for artificial electrochemical muscles (1993)

Electrically conducting conjugated polymers such as polyaniline (PANi) usually show gel properties with volume changes in the range 1-100% in response to some external stimuli. The bending beam method provides an effective and sensitive way to detect and make use of these volume changes. Bipolymer laminate strips (2.5*0.2 cm2), made of a PANi layer (10 mu m thick) and a substrate polyimide layer (45 mu m thick) bound together, were prepared. These strips bend, corresponding to volume changes in the PANi layer, during electrochemical redox of the PANi in aqueous solutions. The extension (<or=1.0 cm) and velocity (<or=48 cm min-1) of the movement of the strips can be controlled by applying appropriate potentials and currents. The authors obtained significant and reversible movement back and forth in seconds.
Drywall State Detection in Image Data for Automatic Indoor Progress Monitoring (2014)

In the context of interior finishing works, delays to the initially planned schedule occur frequently. Since disturbances are unavoidable, a continuous acquisition of the construction progress is essential. Current practice of acquiring the actual construction progress is performed manually and, therefore, is work-intensive and time-consuming. There are approaches that are based on the acquisition of image and video data to derive the current state of objects. Drywall is a very particular case of detectable objects because the extent of the walls frequently do not fit in the sensor of the camera system. Therefore, it requires image features that allow the distinction of different completion states without the feasibility of the investigation of the whole wall. In this paper, the special case of a drywall completion state determination is examined. A method that deals with the automatic detection of the three states - namely: (1) the installation of panels; (2) plastering; and (3) painting - is presented and they are analyzed. Features are derived that allow for the differentiation of the states and a cascaded classification is performed. Therefore, a test set is created consisting of real image data acquired from the finishing of the interior of a building. A Support Vector Machine is trained with the extracted features, and images are tested against the trained classifiers to demonstrate the success of the method.
Localization of Kallikrein Gene Family Proteases in Rat Tissues (1992)

Monoclonal antibodies specific for three kallikrein gene family enzymes (tissue kallikrein, esterase A and tonin) have been used to determine the tissue and cellular distributions of these proteases as well as their association with other relevant molecules (kininogen, kallikrein-binding protein, and Na,K-ATPase alpha-subunit). Secretion of these enzymes from salivary glands was also analyzed. The results of these localization studies provide important clues to the functions of different members of this closely related family of serine proteases.
Nanomaterial‐Based Amplified Transduction of Biomolecular Interactions (2005)

This article reviews progress in the development of nanomaterials for amplified biosensing and discusses different nanomaterial-based bioamplification strategies. Signal amplification has attracted considerable attention for ultrasensitive detection of disease markers and biothreat agents. The emergence of nanotechnology is opening new horizons for highly sensitive bioaffinity and biocatalytic assays and for novel biosensor protocols that employ electronic, optical, or microgravimetric signal transduction. Nucleic acids and antibodies functionalized with metal or semiconductor nanoparticles have been employed as amplifying tags for the detection of DNA and proteins. The coupling of different nanomaterial-based amplification platforms and amplification processes dramatically enhances the intensity of the analytical signal and leads to ultrasensitive bioassays. The successful realization of the new nanoparticle-based signal amplification strategies requires proper attention to nonspecific adsorption issues. The implications of such nanoscale materials on amplified biodetection protocols and on the development of modern biosensors are discussed.
Phosphorylation of Amphiphysin I by Minibrain Kinase/Dual-specificity Tyrosine Phosphorylation-regulated Kinase, a Kinase Implicated in Down Syndrome (2006)

Minibrain kinase/dual-specificity tyrosine phosphorylation-regulated kinase (Mnb/Dyrk1A) is a proline-directed serine/threonine kinase encoded in the Down syndrome critical region of human chromosome 21. This kinase has been shown to phosphorylate dynamin 1 and synaptojanin 1. Here we report that amphiphysin I (Amph I) is also a Mnb/Dyrk1A substrate. This kinase phosphorylated native Amph I in rodent brains and recombinant human Amph I expressed in Escherichia coli. Serine 293 (Ser-293) was identified as the major site, whereas serine 295 and threonine 310 were found as minor kinase sites. In cultured cells, recombinant Amph I was phosphorylated at Ser-293 by endogenous kinase(s). Because mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) has been suggested to phosphorylate Amph I at Ser-293, our efforts addressed whether Ser-293 is phosphorylated in vivo by MAPK/ERK or by Mnb/Dyrk1A. Overnight serum-withdrawal inactivated MAPK/ERK; nonetheless, Ser-293 was phosphorylated in Chinese hamster ovary and SY5Y cells. Epigallocatechin-3-gallate, a potent Mnb/Dyrk1A inhibitor in vitro, apparently reduced the phosphorylation at Ser-293, whereas PD98059, a potent MAPK/ERK inhibitor, did not. High frequency stimulation of mouse hippocampal slices reduced the phosphorylation at Ser-293, albeit in the midst of MAPK/ERK activation. The endophilin binding in vitro was inhibited by phosphorylating Amph I with Mnb/Dyrk1A. However, phosphorylation at Ser-293 did not appear to alter cellular distribution patterns of the protein. Our results suggest that Mnb/Dyrk1A, not MAPK/ERK, is responsible for in vivo phosphorylation of Amph I at Ser-293 and that phosphorylation changes the recruitment of endophilin at the endocytic sites.
Virtual time (1985)

Virtual time is a new paradigm for organizing and synchronizing distributed systems which can be applied to such problems as distributed discrete event simulation and distributed database concurrency control. Virtual time provides a flexible abstraction of real time in much the same way that virtual memory provides an abstraction of real memory. It is implemented using the Time Warp mechanism, a synchronization protocol distinguished by its reliance on lookahead-rollback, and by its implementation of rollback via antimessages.
Stromal Progenitor Cells Present Within Liposuction and Reduction Abdominoplasty Fat for Autologous Transfer to Aged Skin (1999)

Processed liposuction and abdominoplasty processed fat may be ideal for the treatment of wrinkles in aged skin because it contains progenitor stromal cells that are known to be present within the fat and which may be concentrated by processing methods.