gebhart/patrepeval huggingface dataset:
The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. (1983)

An antibody-secreting B cell hybridoma, KJ1-26.1, has been prepared from mice immunized with the T cell hybridoma DO-11.10, which recognizes chicken ovalbumin in association with I-Ad (cOVA/I-Ad). KJ1-26.1 blocks I-restricted antigen recognition by DO-11.10 and a subclone of this T cell hybridoma, DO-11.10.24, which has the same specificity for cOVA/I-Ad as its parent. KJ1-26.1 does not block I-restricted antigen recognition by any other T cell hybridoma tested, including a number of T cell hybridomas closely related to DO-11.10, with similar, but not identical, specificities for antigen/I. Moreover, KJ1-26.1 binds to DO-11.10 and DO-11.10.24, but not to any other T cell hybridomas tested, including three subclones of DO-11.10 that have lost the ability to recognize cOVA/I-Ad. Thus, in every regard KJ1-26.1 appears to be binding to all or part of the receptors for antigen/I on the T cell hybridoma DO-11.10. KJ1-26.1 appears to bind to approximately 15,000 molecules/cell on the surface of DO-11.10. The antibody precipitates an 80,000 dimer from the cells, which on reduction migrates as 40-44,000 monomers. The receptor(s) for antigen/I on DO-11.10 therefore includes molecules with these properties.
Modulation of medullary thyroid carcinoma penetrance suggests the presence of modifier genes in a RET transgenic mouse model. (2003)

We described previously a thyroid phenotype for transgenic mice expressing an activated Ret oncogene driven from a human calcitonin promoter. Medullary thyroid carcinomas (MTC), a tumor of the thyroid parafollicular C cells, occur in this transgenic line with a pathology analogous to that seen in patients with multiple endocrine neoplasia type 2 (MEN2). When the transgene was introgressed onto four different genetic backgrounds, between 0 and 98% of transgenic progeny developed thyroid tumors by 10 months of age, indicating that tumor penetrance could be modulated by genetic background. Furthermore, tumors on the CBA/ca and C57BL/6J backgrounds were significantly larger than those arising in BALB/c transgenic mice. These results are relevant to human MEN2 disease, because this model system may be used to study genes modifying thyroid tumor penetrance in the dominantly inherited human cancer syndrome.
Possible insertion sequences in a mosaic genome organization upstream of the exotoxin A gene in Pseudomonas aeruginosa (1990)

Nucleotide sequence and Southern hybridization data revealed a mosaic genome organization in a region that extends several thousand base pairs upstream of the exotoxin A (toxA) gene in Pseudomonas aeruginosa. An interstrain comparison of DNA in this region showed a pattern of alternating segments of homologous and nonhomologous sequences. Two nonhomologous elements, approximately 1 kilobase pair upstream of the gene in strains PA103 and Ps388, were characterized in more detail. The sequence elements, denoted IS-PA-1 and IS-PA-2 for the different strains, are about 1,000 and 785 base pairs long, respectively, and have 5-base-pair direct repeats at their boundaries, consistent with their being DNA insertion sequences. The distribution of these elements in 34 different strains was determined. IS-PA-1 was found in a single copy upstream of toxA in half of the strains and was found in two copies in four of the strains. Some strains contained neither element, and one strain carried both. The genome of another strain, WR5, which lacks toxA, was shown to contain a 350-base-pair region that was highly homologous to DNA sequences located just upstream of toxA in other strains. The WR5 genome lacked several kilobase pairs of DNA that was found both upstream and downstream of this homologous region in the other strains.
Generation and characterization of ABT-981, a dual variable domain immunoglobulin (DVD-IgTM) molecule that specifically and potently neutralizes both IL-1α and IL-1β (2015)

Interleukin-1 (IL-1) cytokines such as IL-1α, IL-1β, and IL-1Ra contribute to immune regulation and inflammatory processes by exerting a wide range of cellular responses, including expression of cytokines and chemokines, matrix metalloproteinases, and nitric oxide synthetase. IL-1α and IL-1β bind to IL-1R1 complexed to the IL-1 receptor accessory protein and induce similar physiological effects. Preclinical and clinical studies provide significant evidence for the role of IL-1 in the pathogenesis of osteoarthritis (OA), including cartilage degradation, bone sclerosis, and synovial proliferation. Here, we describe the generation and characterization of ABT-981, a dual variable domain immunoglobulin (DVD-Ig) of the IgG1/k subtype that specifically and potently neutralizes IL-1α and IL-1β. In ABT-981, the IL-1β variable domain resides in the outer domain of the DVD-Ig, whereas the IL-1α variable domain is located in the inner position. ABT-981 specifically binds to IL-1α and IL-1β, and is physically capable of binding 2 human IL-1α and 2 human IL-1β molecules simultaneously. Single-dose intravenous and subcutaneous pharmacokinetics studies indicate that ABT-981 has a half-life of 8.0 to 10.4 d in cynomolgus monkey and 10.0 to 20.3 d in rodents. ABT-981 exhibits suitable drug-like-properties including affinity, potency, specificity, half-life, and stability for evaluation in human clinical trials. ABT-981 offers an exciting new approach for the treatment of OA, potentially addressing both disease modification and symptom relief as a disease-modifying OA drug.
<title>Object-based video: extraction tools, evaluation metrics, and applications</title> (2003)

The representation of video information in terms of its content is at the foundation of many multimedia applications, such as broadcasting, content-based information retrieval, interactive video, remote surveillance and entertainment. In particular, object-based representation consists in decomposing the video content into a collection of meaningful objects. This approach offers a broad range of capabilities in terms of access, manipulation and interaction with the visual content. The basic difference when compared with pixel-based procedures is that instead of processing individual pixels, image objects are used in the representation. To exploit the benefits of object-based representation, multimedia applications need automatic techniques for extracting such objects from video data, a problem that still remains largely unsolved. In this paper, we first review the extraction techniques that enable the separation of foreground objects from the background. Their field of applicability and their limitations are discussed. Next, automatic tools for evaluating their performances are introduced. The major applications that benefit from an object-based approach are then analysed. Finally, we discuss some open research issues in object-based video.
TOWARD THE CONQUEST OF BERIBERI (1961)

We assume that this reference book will be a necessity for every medical and many general libraries for it provides ready access to information which would otherwise be very difficult or impossible to obtain.In a fairly thorough search we found only one or two typographical errors.
On Influence and Appropriation (1992)

WHEN MARILYNNE ROBINSON came to Iowa City in the fall of 1990, we promptly planned an interview. So did other readers, for reasons of their own. As we became aware of these other interviews, and were given a chance to see them, we decided each had its own character, that they were complementary far more than redundant, and that a collection of short interviews would offer a fine portrait of a writer we very much admire. That is what we offer here. Each, of course, is much reduced. We have eliminated most, but not quite all of the redundancies and accen
Fluorous Boc (FBoc) Carbamates:  New Amine Protecting Groups for Use in Fluorous Synthesis (2001)

The first fluorous variants of the Boc (tert-butyloxycarbonyl) group have been prepared and tested for their suitability as nitrogen protecting groups. A group with two fluorous chains and an ethylene spacer, (RfCH2CH2)2(CH3)COC(O)-, was readily attached to a representative amine but was difficult to cleave. In contrast, groups with two fluorous chains and a propylene spacer, (RfCH2CH2CH2)2(CH3)COC(O)-, or one fluorous chain and an ethylene spacer, (RfCH2CH2)(CH3)2COC(O)-, were readily formed and cleaved. The fluorous alcohol component of the (F)Boc group can be removed by evaporation and can be recovered and reused. The utility of the new (F)Boc group (C8F17CH2CH2)(CH3)2COC(O)- was demonstrated in 16 and 96 compound library synthesis exercises. Separations can be achieved either by manual, parallel fluorous solid-phase extraction, or automated, serial fluorous chromatography. The results provide additional confirmation of the value of "light" fluorous synthesis techniques, and the new fluorous Boc groups expand the applicability of fluorous synthesis techniques to many classes of nitrogen-containing organic compounds.
Fetal and early life growth and body mass index from birth to early adulthood in 1958 British cohort: longitudinal study (2001)

Maternal weight (or body mass index) largely explains the association between birth weight and adult body mass index, and it may be a more important risk factor for obesity in the child than birth weight. Birth weight and maternal weight seem to modify the effect of childhood linear growth on adult obesity in men. Intergenerational associations between the mother's and her offspring's body mass index seem to underlie the well documented association between birth weight and body mass index. Other measures of fetal growth are needed for a fuller understanding of the role of the intrauterine environment in the development of obesity.
Nonsteroidal Anti‐Inflammatory Drugs: Effects on Kidney Function (1991)

Nonsteroidal anti-inflammatory drugs (NSAIDs) are capable of inducing a variety of renal function abnormalities, particularly in high-risk patients with decreased renal blood perfusion who depend on prostaglandin synthesis to maintain normal renal function. Fluid retention is the most common NSAID-related renal complication, occurring to some degree in virtually all exposed individuals; however, clinically detectable edema occurs in less than 5% of patients and is readily reversible on discontinuation of the NSAID. Other electrolyte complications, notably hyperkalemia, are seen infrequently and occur in specific at-risk patients. The next most worrisome complication is acute deterioration of renal function, which occurs in high-risk patients and is also reversible. Nephrotic syndrome with interstitial nephritis is a rare problem of NSAID use and is reversible. Papillary necrosis is the only permanent complication of NSAIDs and is very rare. Altogether, these renal function abnormalities, with the exception of mild fluid retention, are clinically detectable in approximately 1% of exposed patients. Given the number of patients who take NSAIDs on a prescription or over-the-counter basis, the absolute number of at-risk patients is relatively large. Consequently, an appreciation for the risk factors and pathophysiology of NSAID-induced renal function abnormalities is required for optimal use of these drugs.
The interactions between the fluorescent dye thiazole orange and DNA (1998)

The interaction of the fluorescent dye thiazole orange (TO) with nucleic acids is characterized. It is found that TO binds with highest affinity to double-stranded (ds) DNA [log (K) approximately 5.5 at 100 mM salt], about 5-10 times weaker to single-stranded polypurines, and further 10-1000 times weaker to single-stranded polypyrimidines. TO binds as a monomer to dsDNAs and poly(dA), both as a monomer and as a dimer to poly(dG) and mainly as a dimer to poly(dC) and poly(dT). The fluorescence quantum yield of TO free in solution is about 2 x 10(-4), and it increases to about 0.1 when bound to dsDNA or to poly(dA), and to about 0.4 when bound to poly(dG). Estimated quantum yields of TO bound to poly(dC) and poly(dT) are about 0.06 and 0.01, respectively. The quantum yield of bound TO depends on temperature and decreases about threefold between 5 and 50 degrees C.
Nitric oxide production is required for murine resident peritoneal macrophages to suppress mitogen-stimulated T cell proliferation. Role of IFN-gamma in the induction of the nitric oxide-synthesizing pathway (1991)

Lymphocyte proliferation in Con A- or LPS-stimulated murine splenic cell (SC) cultures was suppressed by the addition of excess macrophages. In Con A-stimulated cultures, suppression was associated with the expression of nitric oxide-synthesizing pathway (NOSP) activity as demonstrated by the accumulation of nitrite, a degradation product of nitric oxide (NO), in the culture supernatants. That NO, a cytotoxic and anti-proliferative metabolite of l-arginine, or other reactive nitrogen intermediates generated through the NOSP mediated the suppressive effect was suggested by the reversal of suppression brought about by the addition of a specific inhibitor of the NOSP (NG-monomethyl-l-arginine acetate) to the culture media. No NOSP activity was detectable in LPS-stimulated SC/macrophage cocultures. The role of T cell-derived IFN-gamma in the induction of the NOSP was investigated by the use of anti-IFN-gamma-mAb. Antibody-treated Con A supernatants failed to induce the NOSP in macrophages, and the addition of the mAb to Con A-stimulated SC/macrophage cocultures obviated the suppressive effects. Indomethacin and catalase only partially restored proliferation in Con A-stimulated SC/macrophage cocultures but were remarkably efficient in preventing macrophage-dependent suppression when LPS was used as the mitogenic stimulus. These results demonstrate a regulatory system of potential relevance in sites of predominant macrophage infiltration by which T cell-derived IFN-gamma activates the production of the mediator, NO, that suppresses T cell proliferation. In addition, these data demonstrate that, although the suppressive effects of excess macrophages appear to be expressed nonspecifically toward both T and B cells, suppression is mediated through a different mechanism in each case.
Inhibition of lectin-induced lymphocyte activation by 2-cyclohexene-1-one: decreased intracellular glutathione inhibits an early event in the activation sequence. (1981)

The importance of normal intracellular glutathione (GSH) levels in the activation of human peripheral blood lymphocytes (PBL) by mitogenic lectins was explored using 2-cyclohexene-1-one (2-CHX-1), an agent that selectively decreases the levels of intracellular GSH. PBL incubated with mitogenic lectins and graded doses of 2-CHX-1 showed a dose-dependent inhibition of activation assayed by [3H]-thymidine uptake and percentage of blast transformation. 2.5 x 10(-5) M 2-CHX-1, a concentration that decreases GSH levels to less than 20% of control, caused an 80 to 90% suppression of both [3H]-thymidine uptake and blast transformation; lower concentrations were less effective. Time course studies showed that 2-CHX-1 was maximally effective only if added during the first 4 hr of culture. At the concentrations used, 2-CHX-1 was not cytotoxic to lectin-stimulated or unstimulated PBL and did not interfere with mitogen-lymphocyte interaction. These results suggest that adequate levels of glutathione are necessary for lymphocyte activation and that the glutathione requirement is exerted in the early activation sequence.
Nonparametric multivariate density estimation: a comparative study (1994)

The paper algorithmically and empirically studies two major types of nonparametric multivariate density estimation techniques, where no assumption is made about the data being drawn from any of known parametric families of distribution. The first type is the popular kernel method (and several of its variants) which uses locally tuned radial basis (e.g., Gaussian) functions to interpolate the multidimensional density; the second type is based on an exploratory projection pursuit technique which interprets the multidimensional density through the construction of several 1D densities along highly "interesting" projections of multidimensional data. Performance evaluations using training data from mixture Gaussian and mixture Cauchy densities are presented. The results show that the curse of dimensionality and the sensitivity of control parameters have a much more adverse impact on the kernel density estimators than on the projection pursuit density estimators.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Interferon alpha 2b but not interferon alpha 2a detected in human genomic DNA. (1993)

The presence of the genes for human interferon (IFN)-alpha 2a and IFN-alpha 2b subvariants in human genomic DNA was studied using polymerase chain reaction (PCR). The respective genes differ in the coding sequence only at the position 137, adenine (2A) being substituted by guanine (2B). IFN-alpha 2-sequences were selectively amplified from the placental genomic DNA using specific primers. When sequencing the mixture of PCR-derived clones, at the position 137 guanine, specific for subvariant 2b could be detected. This indicates that, at least in the genome analyzed, only sequence coding for IFN-alpha 2b was found. Our finding supports the view, that genes for IFN-alpha 2a and IFN-alpha 2b seem to be rather allelic than occurring at distinct loci.
Synthesis of magnesium hydride by the reaction of phenylsilane and dibutylmagnesium (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of magnesium hydride by the reaction of phenylsilane and dibutylmagnesiumMichael J. MichalczykCite this: Organometallics 1992, 11, 6, 2307–2309Publication Date (Print):June 1, 1992Publication History Published online1 May 2002Published inissue 1 June 1992https://pubs.acs.org/doi/10.1021/om00042a055https://doi.org/10.1021/om00042a055research-articleACS PublicationsRequest reuse permissionsArticle Views707Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
ShelfNet for Fast Semantic Segmentation (2018)

In this paper, we present ShelfNet, a novel architecture for accurate fast semantic segmentation. Different from the single encoder-decoder structure, ShelfNet has multiple encoder-decoder branch pairs with skip connections at each spatial level, which looks like a shelf with multiple columns. The shelf-shaped structure can be viewed as an ensemble of multiple deep and shallow paths, thus improving accuracy. We significantly reduce computation burden by reducing channel number, at the same time achieving high accuracy with this unique structure. In addition, we propose a shared-weight strategy in the residual block which reduces parameter number without sacrificing performance. Compared with popular non real-time methods such as PSPNet, our ShelfNet achieves 4$\times$ faster inference speed with similar accuracy on PASCAL VOC dataset. Compared with real-time segmentation models such as BiSeNet, our model achieves higher accuracy at comparable speed on the Cityscapes Dataset, enabling the application in speed-demanding tasks such as street-scene understanding for autonomous driving. Furthermore, our ShelfNet achieves 79.0\% mIoU on Cityscapes Dataset with ResNet34 backbone, outperforming PSPNet and BiSeNet with large backbones such as ResNet101. Through extensive experiments, we validated the superior performance of ShelfNet. We provide link to the implementation \url{https://github.com/juntang-zhuang/ShelfNet-lw-cityscapes}.
NMR, Relaxometric, and Structural Studies of the Hydration and Exchange Dynamics of Cationic Lanthanide Complexes of Macrocyclic Tetraamide Ligands (1999)

The solution structure and dynamics of metal-bound water exchange have been investigated in a series of lanthanide complexes of primary, secondary, and tertiary tetraamide derivatives of 1,4,7,10-tetraazacyclododecane. In the gadolinium complexes at ambient pH, water exchange lifetimes (τm) determined by 17O NMR were sufficiently long (19 μs for [Gd·2]3+, 298 K, 17 μs for [Gd·3]3+, and 8 μs for [Gd·4]3+) to limit the measured relaxivity. Direct 1H NMR observation of the bound water resonance is possible for the corresponding Eu complexes at low temperature in CD3CN, and the rate of water proton exchange is about 50 times faster in the twisted square antiprismatic isomer (m) than in the isomeric square antiprismatic (M) complex. The ratio of these two isomers in solution is sensitive to the steric demand of the amide substituent, with m/M = 2 for [Eu·4]3+, but 0.25 for [Eu·2]3+. The slowness of coordinated water exchange has allowed the rate of prototropic exchange to be studied: in basic media deprotonation of the bound water molecule or of proximate ligand amide NH protons leads to relaxivity enhancements, whereas in acidic media, hydration around the strongly ion-paired complexes is perturbed, facilitating water exchange. The X-ray crystal structure of ligand 3 reveals a hydrogen-bonded structure with two pairs of ring N-substituents related in a trans arrangement, contrasting with the structure of diprotonated DOTA in which the ligand is predisposed to bind metal ions. In the dysprosium complex [Dy·3·OH2](PF6)3, the metal ion adopts a regular monocapped square antiprismatic coordination geometry, with a water Dy−O bond length of 2.427(3) Å, and a PF6 counterion is strongly hydrogen-bonded to this bound water molecule.
Prospective randomized study of the role of N-acetyl cysteine in reversing doxorubicin-induced cardiomyopathy (1982)

We conducted a randomized prospective trial in 19 disease-free soft tissue sarcoma patients with doxorubicin-induced cardiomyopathy identified by ECG radionuclide angiography at rest and during exercise to determine the efficacy of the free radical scavenger, N-Acetyl Cysteine (NAC), in reversing the drug's cardiotoxic effect. Of the 19 patients, 11 received oral NAC (5.5 gm/m2 daily for 30 days) and eight patients served as controls. Patients were stratified for age less than greater than 45 years, time from final dose of doxorubicin to randomization less than greater than 8 months, and history of treatment with mediastinal irradiation. The two groups were well-matched for all parameters. Cumulative mean doxorubicin dose (523 mg/m2 and 532 mg/m2) and range 500-600 mg/m2 was comparable. Left ventricular (LV) ejection fraction before randomization was not significantly different between the two groups either at rest (39 +/- 10% control, 38 +/- 13% NAC) or during exercise (38 +/- 12% control, 35 +/- 11% NAC). Neither rest nor exercise ejection fraction values changed significantly in either group between prerandomization and 1-month postrandomization studies. Late studies performed in seven NAC patients 3-5 months after randomization revealed no difference in LV ejection fraction compared to 1-month postrandomization values. Clinical course in patients with overt congestive heart failure was similar in both groups. LV function did not return to normal in any patient in either group. We conclude that N-Acetyl Cysteine has no effect in reversing long standing doxorubicin-induced cardiomyopathy.