gebhart/patrepeval huggingface dataset:
Portrait painting using active templates (2011)

Portraiture plays a substantial role in traditional painting, yet it has not been studied in depth in painterly rendering research. The difficulty in rendering human portraits is due to our acute visual perception to the structure of human face. To achieve satisfactory results, a portrait rendering algorithm should account for facial structure. In this paper, we present an example-based method to render portrait paintings from photographs, by transferring brush strokes from previously painted portrait templates by artists. These strokes carry rich information about not only the facial structure but also how artists depict the structure with large and decisive brush strokes and vibrant colors. With a dictionary of portrait painting templates for different types of faces, we show that this method can produce satisfactory results.
A mathematical analysis of the DCT coefficient distributions for images (2000)

Over the past two decades, there have been various studies on the distributions of the DCT coefficients for images. However, they have concentrated only on fitting the empirical data from some standard pictures with a variety of well-known statistical distributions, and then comparing their goodness of fit. The Laplacian distribution is the dominant choice balancing simplicity of the model and fidelity to the empirical data. Yet, to the best of our knowledge, there has been no mathematical justification as to what gives rise to this distribution. We offer a rigorous mathematical analysis using a doubly stochastic model of the images, which not only provides the theoretical explanations necessary, but also leads to insights about various other observations from the literature. This model also allows us to investigate how certain changes in the image statistics could affect the DCT coefficient distributions.
A phase II study of autologous tumor infiltrating lymphocytes (TIL, LN-144/LN-145) in patients with solid tumors. (2019)

TPS2648 Background: Adoptive cell therapy (ACT) with tumor infiltrating lymphocytes (TIL) has demonstrated durable complete responses in immunogenic tumors with high mutational burden in metastatic melanoma patients who had not received prior immune checkpoint inhibitors (ICI); CR rate 24%. Pembrolizumab is an approved agent for the treatment of metastatic melanoma and head &amp; neck cancers, among others. Further, ICI have been reported to potentially enhance the efficacy of TIL therapy. One aim of this study is to improve the efficacy response for early line patients by combining TIL with anti-PD-1 in ICI-naïve patients with metastatic melanoma (Cohort 1) and head &amp; neck cancers (Cohorts 2). In Cohort 3, TIL therapy alone is offered to NSCLC patients who have received prior systemic therapy, including ICI. Methods: IOV-COM-202 is a prospective, Phase 2 multicenter, open-label study in which 36 patients (12 per cohort) are to be enrolled in one of three cohorts; Cohorts 1 and 2: TIL therapy in combination with pembrolizumab, or Cohort 3: TIL therapy alone. Patients will have tumors resected at local centers and shipped to a central GMP facility to undergo a 22-day manufacturing process that yields cryopreserved infusion product (LN-144/LN-145) that is shipped back to treating center. All patients receive TIL therapy consisting of 1 week of preconditioning cyclophosphamide/fludarabine, followed by a single infusion of LN-144/LN-145 (Day 0) and up to 6 doses of IL-2 (600,000 IU/kg). Patients in Cohorts 1 and 2 also receive pembrolizumab on Day -1 and then Q3W for up to 2 years or until disease progression or acceptable toxicity. Co-primary endpoints for each cohort are objective response rate (ORR) per RECIST 1.1, and safety (grade ≥ 3 TEAE). Eligibility criteria: Cohorts 1 (melanoma) and 2 (head &amp; neck): patients must not have received prior ICI (eg, anti-PD-1, anti-CTLA-4) and may have received up to 3 lines of prior systemic therapy, Cohort 3 (NSCLC): patients must have received 1-3 prior lines of systemic therapy including ICI. After tumor resection for TIL manufacturing, patients must have additional measurable disease for assessment per RECIST 1.1. Adequate bone marrow/organ function and ECOG PS of 0 or 1 is required. Clinical trial information: NCT03645928.
Deletion of the vaccinia virus B5R gene encoding a 42-kilodalton membrane glycoprotein inhibits extracellular virus envelope formation and dissemination (1993)

The structure, formation, and function of the virion membranes are among the least well understood aspects of vaccinia virus replication. In this study, we investigated the role of gp42, a glycoprotein component of the extracellular enveloped form of vaccinia virus (EEV) encoded by the B5R gene. The B5R gene was deleted by homologous recombination from vaccinia virus strains IHD-J and WR, which produce high and low levels of EEV, respectively. Isolation of recombinant viruses was facilitated by the insertion into the genome of a cassette containing the Escherichia coli gpt and lacZ genes flanked by the ends of the B5R gene to provide simultaneous antibiotic selection and color screening. Deletion mutant viruses of both strains formed tiny plaques, and those of the IHD-J mutant lacked the characteristic comet shape caused by release of EEV. Nevertheless, similar yields of intracellular infectious virus were obtained whether cells were infected with the B5R deletion mutants or their parental strains. In the case of IHD-J, however, this deletion severely reduced the amount of infectious extracellular virus. Metabolic labeling studies demonstrated that the low extracellular infectivity corresponded with a decrease in EEV particles in the medium. Electron microscopic examination revealed that mature intracellular naked virions (INV) were present in cells infected with mutant virus, but neither membrane-wrapped INV nor significant amounts of plasma membrane-associated virus were observed. Syncytium formation, which occurs in cells infected with wild-type WR and IHD-J virus after brief low-pH treatment, did not occur in cells infected with the B5R deletion mutants. By contrast, syncytium formation induced by antibody to the viral hemagglutinin occurred, suggesting that different mechanisms are involved. When assayed by intracranial injection into weanling mice, both IHD-J and WR mutant viruses were found to be significantly attenuated. These findings demonstrate that the 42-kDa glycoprotein of the EEV is required for efficient membrane enwrapment of INV, externalization of the virus, and transmission and that gp42 contributes to viral virulence in strains producing both low and high levels of EEV.
Asymmetric synthesis via axially dissymetric molecules. 2. A highly efficient synthesis of prostaglandin intermediates possessing the 15S configuration (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric synthesis via axially dissymetric molecules. 2. A highly efficient synthesis of prostaglandin intermediates possessing the 15S configurationR. Noyori, I. Tomino, and M. NishizawaCite this: J. Am. Chem. Soc. 1979, 101, 19, 5843–5844Publication Date (Print):September 1, 1979Publication History Published online1 May 2002Published inissue 1 September 1979https://pubs.acs.org/doi/10.1021/ja00513a072https://doi.org/10.1021/ja00513a072research-articleACS PublicationsRequest reuse permissionsArticle Views597Altmetric-Citations86LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Influence of DNA Sequence on the Positioning of RecA Monomers in RecA-DNA Cofilaments (2002)

We show that certain DNA sequences have the ability to influence the positioning of RecA monomers in RecA-DNA complexes. A tendency for RecA monomers to be phased was observed in RecA protein complexes with several oligonucleotides containing a recombinational hotspot sequence, the chi-site from Escherichia coli. This influence was observed in both the 5' to 3' and 3' to 5' directions with respect to chi. A 5'-end phosphate group and probably some other features in DNA also influence the phasing of RecA monomers. We conclude that natural DNAs contain a number of features that influence the positioning of RecA monomers. The ability of specific DNA sequences to influence the positioning of RecA monomers demonstrates some specificity in the binding of individual bases at different sites within a RecA monomer and, most likely, reflects the stereochemical non-equivalence of these sites. The possible biological implications of the phasing of RecA monomers in presynaptic DNA-protein cofilaments are discussed.
Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. (1994)

The E6 protein of the oncogenic human papillomavirus types 16 and 18 facilitates the rapid degradation of the tumor-suppressor protein p53 via the ubiquitin-dependent proteolytic pathway. The E6 protein binds to a cellular protein of 100 kDa termed E6-AP. The complex of E6 and E6-AP specifically interacts with p53 and induces the ubiquitination of p53 in a reaction which requires the ubiquitin-activating enzyme (E1) and a cellular fraction thought to contain a mammalian ubiquitin-conjugating enzyme (E2). This mammalian E2 activity could be replaced with bacterially expressed UBC8 from Arabidopsis thaliana, which belongs to a subfamily of E2s including yeast UBC4 and UBC5 which are highly conserved at the amino acid level. In this paper we describe the cloning of a human cDNA encoding a human E2 that we have designated UbcH5 and that is related to Arabidopsis UBC8 and the other members of this subfamily. We demonstrate that UbcH5 can function in the E6/E6-AP-induced ubiquitination of p53.
Microelectromechanical Optical Beam Steering Systems (1997)

Abstract : The development of microelectromechanical systems (MEMS) has matured to the point where the fabrication of micron sized devices is feasible. State of the art MEMS construction processes now support the fabrication of novel optical devices that could not previously be built. This dissertation reports on the development of innovative micro optical devices such as Variable Blaze Gratings (VBGs) using state of the art MEMS construction processes. The principle application of the micro optical devices described in this dissertation is steering optical beams; however other applications such as spectral analysis are identified. Specific optical beam steering systems developed and characterized in this work include: optical phased arrays, VBGs, decentered microlens arrays, and integrated Vertical Cavity Surface Emitting Laser (VCSEL) micro mirror systems. Optical power induced damage to micro machined mirrors is also modeled and tested. In addition a number of new MEMS devices were created during this research including: double hot arm thermal actuators, optical hybrid devices such as variable blaze phased arrays and focusing micro mirrors, and non-linear flexures. Extensive Fourier analysis and optical testing of the micro optical devices was used to verify device operation.
Characterization and PEMFC Testing of Pt[sub 1−x]M[sub x] (M=Ru,Mo,Co,Ta,Au,Sn) Anode Electrocatalyst Composition Spreads (2007)

random alloy samples, covering most of the binary composition range, have been prepared via magnetron sputtering. The alloys were deposited through shadow masks onto 3M nanostructured thin-film catalyst support for testing in a 64-electrode polymer electron membrane fuel cell (PEMFC). CO stripping voltammograms and hydrogen oxidation polarization curves with pure hydrogen and with reformate containing up to CO were measured on all the samples. In agreement with reports in the literature, Ru, Mo, and Sn were found to improve the CO tolerance of Pt, although the intrinsic hydrogen oxidation activity of Pt decreased significantly as the Sn content increased. The addition of Co to Pt had no impact on CO tolerance, possibly because of loss of surface Co through dissolution in the fuel cell. The addition of Au to Pt led to an increase in hydrogen oxidation overpotential when CO was present. Small amounts of Ta gave a small reduction in hydrogen oxidation overpotential in the presence of CO, but the overpotentials were still too high for practical application in a reformate-fed fuel cell.
GD1α‐replica peptides functionally mimic GD1α, an adhesion molecule of metastatic tumor cells, and suppress the tumor metastasis (1998)

A novel peptide technology to produce mimicking peptides of carbohydrate moiety (which we propose to name glyco-replica peptides) is a useful tool to elucidate the functions of glycoconjugate. Carbohydrate moiety of ganglioside GD1alpha functions as a molecule involved in the adhesion between murine highly metastatic lymphoma RAW117-H10 cells and hepatic sinusoidal endothelial (HSE) cells. To prepare peptides which mimic the carbohydrate structure of GD1alpha, phage clones expressing peptides which bound to a monoclonal antibody against GD1alpha (KA17) were isolated from a phage-displayed random peptide library. Four phage clones having affinity to the monoclonal antibody KA17 were isolated, and these clones showed inhibitory effect on the binding of KA17 to GD1alpha. The amino acid sequences of the displayed pentadecamers were determined, and one of the phages displaying sequence WHWRHRIPLQLAAGR bound to HSE cells directly and showed the highest inhibitory effect on the adhesion between RAW117-H10 cells and HSE cells. The synthesized peptides having the same sequences to the displayed 15mers in the four isolated phage clones also showed the inhibitory effect on the adhesion of RAW117-H10 cells to HSE cells, and, again, the WHWRHRIPLQLAAGR peptide showed the highest inhibitory effect. Furthermore, intravenous injection of the peptide brought almost complete inhibition of the metastasis of RAW117-H10 cells to lung and spleen, and about 50% inhibition of the liver metastasis. These results indicate that GD1alpha plays an important role for metastasis of RAW117-H10 cells, and the peptides obtained by the present procedure are able to mimic the functional role of the glycoconjugate.
Autoreactivity and Exceptional CDR Plasticity (but Not Unusual Polyspecificity) Hinder Elicitation of the Anti-HIV Antibody 4E10 (2013)

The broadly-neutralizing anti-HIV antibody 4E10 recognizes an epitope in the membrane-proximal external region of the HIV envelope protein gp41. Previous attempts to elicit 4E10 by vaccination with envelope-derived or reverse-engineered immunogens have failed. It was presumed that the ontogeny of 4E10-equivalent responses was blocked by inherent autoreactivity and exceptional polyreactivity. We generated 4E10 heavy-chain knock-in mice, which displayed significant B cell dysregulation, consistent with recognition of autoantigen/s by 4E10 and the presumption that tolerance mechanisms may hinder the elicitation of 4E10 or 4E10-equivalent responses. Previously proposed candidate 4E10 autoantigens include the mitochondrial lipid cardiolipin and a nuclear splicing factor, 3B3. However, using carefully-controlled assays, 4E10 bound only weakly to cardiolipin-containing liposomes, but also bound negatively-charged, non-cardiolipin-containing liposomes comparably poorly. 4E10/liposome binding was predominantly mediated by electrostatic interactions rather than presumed hydrophobic interactions. The crystal structure of 4E10 free of bound ligands showed a dramatic restructuring of the combining site, occluding the HIV epitope binding site and revealing profound flexibility, but creating an electropositive pocket consistent with non-specific binding of phospholipid headgroups. These results strongly suggested that antigens other than cardiolipin mediate 4E10 autoreactivity. Using a synthetic peptide library spanning the human proteome, we determined that 4E10 displays limited and focused, but unexceptional, polyspecificity. We also identified a novel autoepitope shared by three ER-resident inositol trisphosphate receptors, validated through binding studies and immunohistochemistry. Tissue staining with 4E10 demonstrated reactivity consistent with the type 1 inositol trisphosphate receptor as the most likely candidate autoantigen, but is inconsistent with splicing factor 3B3. These results demonstrate that 4E10 recognition of liposomes competes with MPER recognition and that HIV antigen and autoepitope recognition may be distinct enough to permit eliciting 4E10-like antibodies, evading autoimmunity through directed engineering. However, 4E10 combining site flexibility, exceptional for a highly-matured antibody, may preclude eliciting 4E10 by conventional immunization strategies.
A network of transcriptional and signaling events is activated by FGF to induce chondrocyte growth arrest and differentiation (2003)

Activating mutations in FGF receptor 3 (FGFR3) cause several human dwarfism syndromes by affecting both chondrocyte proliferation and differentiation. Using microarray and biochemical analyses of FGF-treated rat chondrosarcoma chondrocytes, we show that FGF inhibits chondrocyte proliferation by initiating multiple pathways that result in the induction of antiproliferative functions and the down-regulation of growth-promoting molecules. The initiation of growth arrest is characterized by the rapid dephosphorylation of the retinoblastoma protein (pRb) p107 and repression of a subset of E2F target genes by a mechanism that is independent of cyclin E-Cdk inhibition. In contrast, hypophosphorylation of pRb and p130 occur after growth arrest is first detected, and may contribute to its maintenance. Importantly, we also find a number of gene expression changes indicating that FGF promotes many aspects of hypertrophic differentiation, a notion supported by in situ analysis of developing growth plates from mice expressing an activated form of FGFR3. Thus, FGF may coordinate the onset of differentiation with chondrocyte growth arrest in the developing growth plate.
Hemodynamic Effects of a New Percutaneous Circulatory Support Device in a Left Ventricular Failure Model (2003)

We have tested a new percutaneous circulatory support device in seven anesthetized calves with induced left ventricular failure. The device is based on a flexible catheter with a foldable propeller and cage at the distal end. The rotation of the propeller (1,000-15,000 rpm) is transmitted from a drive unit at the proximal end to the propeller by way of a rotating wire inside the catheter. This also contains an umbrella-like mechanism to open the pump head from the folded (diameter 4.6 mm) to the active position. The rotation of the propeller creates a pressure drop in front of the propeller and a pressure rise behind. Heart failure was induced with metoprolol and verapamil in combination with a VVI pacemaker to create a left atrial pressure greater than 20 mm Hg. A centrifugal pump was used to bypass the right ventricle and to ensure a sufficient filling of the left ventricle. After baseline recordings, the pump was run at 14,000 rpm, and the hemodynamic response was compared with the baseline. A 24 +/- 10 mm Hg pressure gradient was generated across the pump, resulting in a drop in the right carotid artery mean pressure from 80 +/- 11 to 71 +/- 13 mm Hg (p = 0.008) and a drop in the left ventricular systolic pressure from 109 +/- 17 to 100 +/- 19 mm Hg (p = 0.004). The pressure in the left atrium decreased from 25 +/- 3 to 20 +/- 5 mm Hg (p = 0.008). The mean femoral pressure increased from 78 +/- 10 to 95 +/- 20 mm Hg (p = 0.005). A moderate reduction in the right carotid flow was observed (15%, p = 0.029), whereas no significant changes were found in the coronary flow, the flow in the right femoral artery, or in the left kidney. The device showed a significant unloading of the left ventricle and an increased perfusion pressure for the lower part of the body. The moderate changes in flow were probably caused by still active autoregulation, and this needs to be tested with more pronounced circulatory failure.
A different best rigid-body molecular fit routine (1977)

A different algorithm which gives a least-squares fit between two sets of atoms is described [cf. Nyburg (1974). Acta Cryst. B30, 251-253]. With this algorithm the coordinates of the moving set of atoms are changed only once.
The dielectric constant in a thermal phase transition magnetic material composed of rubidium manganese hexacyanoferrate observed by spectroscopic ellipsometry (2005)

In this work, the dielectric constant of rubidium manganese hexacyanoferrate was observed in the ultraviolet, visible, and near-infrared regions using spectroscopic ellipsometry (SE). The SE data for ε drastically varied with the phase transition between the high-temperature and low-temperature phases, e.g., the gain of the imaginary part of the dielectric constant (ε″) changed 2900% at 520 nm. In a contrasting experiment, the dielectric constant in the 103–105 Hz frequency range was also measured by an LCR meter and the gain factors of ε were approximately 30%. This large difference in the gain factors between SE and LCR methods is caused by the different resonance types, i.e., the SE method observes electronic resonance such as intervalence transfer band, while the LCR method observes dipolar resonance. The present work is the first SE observation of ε in metal complexes, and the ε data collected by an LCR meter are first to be observed in Prussian blue analogs.
Estrogen Plus Progestin and Breast Cancer Incidence and Mortality in Postmenopausal Women (2010)

clinicaltrials.gov Identifier: NCT00000611.
The predetermination of embryonic sex using flow cytometrically separated X and Y spermatozoa (1996)

A review is given of the predetermination of sex in various domestic animals and in the human using sperm samples enriched for X- or Y-chromosome bearing spermatozoa obtained by flow cytometry and cell sorting. A comparison of other putative methods of sperm separation is made. In separating human X and Y spermatozoa, measurements of the DNA content in each individual gamete using the Hoechst fluorochrome 33342 remains the only validated method. The difference in DNA content between human X and Y spermatozoa is approximately 2.8%, and cell sorters have been adapted to take account of this and the asymmetrical nature of the sperm head. DNA analyses and PCR have been used to validate the method for animal spermatozoa. In the human, fluorescence in-situ hybridization (FISH) has confirmed sorting accuracy. Many correctly-diagnosed normal offspring have been born in various animal species and any potential mutagenic or cytotoxic effects are being closely monitored as are the cost and efficiency of the technology.