gebhart/patrepeval huggingface dataset:
A Self-commissioning Notch Filter for Active Damping in a Three-Phase LCL -Filter-Based Grid-Tie Converter (2014)

LCL-filters are a cost-effective solution to mitigate harmonic current content in grid-tie converters. In order to avoid stability problems, the resonance frequency of LCL-filters can be damped with active techniques that remove dissipative elements but increase control complexity. A notch filter provides an effective solution, however tuning the filter requires considerable design effort and the variations in the grid impedance limit the LCL-filter robustness. This paper proposes a straightforward tuning procedure for a notch filter self-commissioning. In order to account for the grid inductance variations, the resonance frequency is estimated and later used for tuning the notch filter. An estimation for the maximum value of the proportional gain to excite the resonance is provided. The resonance frequency is calculated using the Goertzel algorithm, which requires little extra computational resources in the existing control processor. The discrete Fourier transform coefficients are therefore obtained, with less calculations than the running sum implementation and less memory requirements than with the fast Fourier transform (FFT). Thus, the self-commissioning technique is robust to grid impedance variations due to its ability to tune the grid-tie inverter on-site. Finally, the analysis is validated with both simulation and experiments.
Tumor-Associated Tn-MUC1 Glycoform Is Internalized through the Macrophage Galactose-Type C-Type Lectin and Delivered to the HLA Class I and II Compartments in Dendritic Cells (2007)

Abstract The type of interaction between tumor-associated antigens and specialized antigen-presenting cells such as dendritic cells (DCs) is critical for the type of immunity that will be generated. MUC1, a highly O-glycosylated mucin, is overexpressed and aberrantly glycosylated in several tumor histotypes. This results in the expression of tumor-associated glycoforms and in MUC1 carrying the tumor-specific glycan Tn (GalNAcα1-O-Ser/Thr). Glycopeptides corresponding to three tandem repeats of MUC1, enzymatically glycosylated with 9 or 15 mol of GalNAc, were shown to specifically bind and to be internalized by immature monocyte-derived DCs (iDCs). Binding required calcium and the GalNAc residue and was competed out by GalNAc polymer and Tn-MUC1 or Tn-MUC2 glycopeptides. The macrophage galactose-type C-type lectin (MGL) receptor expressed on iDCs was shown to be responsible for the binding. Confocal analysis and ELISA done on subcellular fractions of iDCs showed that the Tn-MUC1 glycopeptides colocalized with HLA class I and II compartments after internalization. Importantly, although Tn-MUC1 recombinant protein was bound and internalized by MGL, the glycoprotein entered the HLA class II compartment, but not the HLA class I pathway. These data indicate that MGL expressed on iDCs is an optimal receptor for the internalization of short GalNAcs carrying immunogens to be delivered into HLA class I and II compartments. Such glycopeptides therefore represent a new way of targeting the HLA class I and II pathways of DCs. These results have possible implications in designing cancer vaccines. [Cancer Res 2007;67(17):8358–67]
Comparison of Nasal and Oral Inhalation during Exhaled Breath Condensate Collection (2003)

Analysis of exhaled breath condensate is a method for noninvasive assessment of the lung. Condensate can be collected with a nose clip (subjects inhale and exhale via the mouth) or without it (subjects inhale via the nose and exhale via the mouth), but the mode of inhalation may influence condensate volume and mediator levels. We compared condensate volume and adenosine, ammonia, and thromboxane B2 levels in young healthy volunteers (n = 25) in samples collected for 10 minutes from subjects with or without a nose clip. Patients with allergic rhinitis (n = 8) were also studied to assess the effect of upper airway inflammation on mediator levels. Adenosine, ammonia, and thromboxane B2 levels were determined by HPLC, spectrophotometry, and radioimmunoassay, respectively. Volume of condensate was significantly higher without nose clip than that with nose clip (mean +/- SD, 2321 +/- 736 microl and 1746 +/- 400 microl, respectively; p = 0.0001). We found no significant difference in any mediator levels between these two collection modes in healthy volunteers, but adenosine showed a tendency to differ between oral and nasal inhalation in patients with allergic rhinitis. Our data indicate that whereas a greater volume of condensate can be obtained when subjects inhale through their noses, the mode of inhalation does not influence mediator levels in young healthy volunteers, but may affect these levels in patients with allergic rhinitis.
Isolation and functional characterization of human erythroblasts at distinct stages: implications for understanding of normal and disordered erythropoiesis in vivo (2013)

Terminal erythroid differentiation starts from morphologically recognizable proerythroblasts that proliferate and differentiate to generate red cells. Although this process has been extensively studied in mice, its characterization in humans is limited. By examining the dynamic changes of expression of membrane proteins during in vitro human terminal erythroid differentiation, we identified band 3 and α4 integrin as optimal surface markers for isolating 5 morphologically distinct populations at successive developmental stages. Functional analysis revealed that these purified cell populations have distinct mitotic capacity. Use of band 3 and α4 integrin enabled us to isolate erythroblasts at specific developmental stages from primary human bone marrow. The ratio of erythroblasts at successive stages followed the predicted 1:2:4:8:16 pattern. In contrast, bone marrows from myelodysplastic syndrome patients exhibited altered terminal erythroid differentiation profiles. Thus, our findings not only provide new insights into the genesis of the red cell membrane during human terminal erythroid differentiation but also offer a means of isolating and quantifying each developmental stage during terminal erythropoiesis in vivo. Our findings should facilitate a comprehensive cellular and molecular characterization of each specific developmental stage of human erythroblasts and should provide a powerful means of identifying stage-specific defects in diseases associated with pathological erythropoiesis.
Library Generation by Gene Shuffling (2014)

This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer-less PCR. The resulting chimeric genes can then be screened or selected for a desired function.
Multilinear array manifold interpolation (1992)

Two algorithmic solutions for interpolating between array manifold grid points are presented. The algorithms are for sensor arrays in vector or in scalar wavefields. The algorithms make successful use of the condition that the reciprocal DOA (direction-of-arrival) spectrum is a multilinear function in the small, i.e. over the region of adjacent grid points. Since polarization is a linear parameter subspace, a DOA spectrum can be computed without recourse to the unknown polarizations. Then, vector interpolation is replaced by bivector interpolation to address the radio wavefield case. The approach distinguishes between sensor arrays in scalar wavefields (e.g. acoustic) and those in vector wavefields (e.g. electromagnetic). Since both storage and computational load vary with the reciprocal of the grid spacing or its square, the design relationship between desired accuracy and the required array manifold grid spacing is given.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Convergent total synthesis of (.+-.)-colchicine and (.+-.)-desacetamidoisocolchicine (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConvergent total synthesis of (.+-.)-colchicine and (.+-.)-desacetamidoisocolchicineD. A. Evans, S. P. Tanis, and D. J. HartCite this: J. Am. Chem. Soc. 1981, 103, 19, 5813–5821Publication Date (Print):September 1, 1981Publication History Published online1 May 2002Published inissue 1 September 1981https://pubs.acs.org/doi/10.1021/ja00409a032https://doi.org/10.1021/ja00409a032research-articleACS PublicationsRequest reuse permissionsArticle Views1768Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Rapid histology of laryngeal squamous cell carcinoma with deep-learning based stimulated Raman scattering microscopy (2019)

Maximal resection of tumor while preserving the adjacent healthy tissue is particularly important for larynx surgery, hence precise and rapid intraoperative histology of laryngeal tissue is crucial for providing optimal surgical outcomes. We hypothesized that deep-learning based stimulated Raman scattering (SRS) microscopy could provide automated and accurate diagnosis of laryngeal squamous cell carcinoma on fresh, unprocessed surgical specimens without fixation, sectioning or staining. Methods: We first compared 80 pairs of adjacent frozen sections imaged with SRS and standard hematoxylin and eosin histology to evaluate their concordance. We then applied SRS imaging on fresh surgical tissues from 45 patients to reveal key diagnostic features, based on which we have constructed a deep learning based model to generate automated histologic results. 18,750 SRS fields of views were used to train and cross-validate our 34-layered residual convolutional neural network, which was used to classify 33 untrained fresh larynx surgical samples into normal and neoplasia. Furthermore, we simulated intraoperative evaluation of resection margins on totally removed larynxes. Results: We demonstrated near-perfect diagnostic concordance (Cohen's kappa, κ > 0.90) between SRS and standard histology as evaluated by three pathologists. And deep-learning based SRS correctly classified 33 independent surgical specimens with 100% accuracy. We also demonstrated that our method could identify tissue neoplasia at the simulated resection margins that appear grossly normal with naked eyes. Conclusion: Our results indicated that SRS histology integrated with deep learning algorithm provides potential for delivering rapid intraoperative diagnosis that could aid the surgical management of laryngeal cancer.
THE BIOTECHNOLOGY FACILITIES AT SOUSTONS FOR BIOMASS CONVERSION (1992)

A technical description of the large scale biotechnology facilities for biomass conversion at Soustons (Landes, France) is presented. The equipments, designed for versatility and operational flexibility, include the basic units for implementing the individual steps of lignocellulosic biomass enzymatic hydrolysis and fermentation: steam explosion pretreatment, aerobic fermentation for cellulase production, enzymatic hydrolysis, anaerobic fermentation. The main results of the large scale development of a process for production of acetone-butanol from lignocellulosic agricultural residues are presented as well as other experimentations in the field of biomass valorization. In this area the present programme is directed to conversion of lignocellulosic biomass into ethanol but the activity of the facilities also includes development work and experimental productions in a wide range of industrial fermentations.
Role of urinary trypsin inhibitor in the maintenance of pregnancy in mice (1996)

Urinary trypsin inhibitor prevents the pathogenicity of preterm delivery through the suppression of cytokine production.
A Differential Colpitts-VCO Circuit Suitable for Sub-1V Low Phase Noise Operation (2011)

This paper proposes a differential Colpitts-VCO circuit suitable for low phase noise oscillation at the sub-1V supply voltage. Oscillation with low phase noise at the sub-1V supply voltage is facilitated by employing inductors as the current sources of the proposed circuit. One of the two feedback capacitors of the single-ended Colpitts oscillator in the proposed circuit is replaced with the MOS varactor in order to further reduce the resonator loss. Post-layout simulation results using a RF CMOS technology show that the phase noises at the 1MHz offset frequency of the proposed circuit oscillating at the sub-1V supply voltages of 0.6 to 0.9 V are at least 7 dBc/Hz lower than those of the well-known cross-coupled differential VCO.
Biphasic Sodium Fluoride Effects on Bone and Bone Mineral: A Review (1995)

This paper reviews the clinical and experimental findings on the effects of sodium fluoride (NaF) on human and animal bone. NaF has been shown to cause a significant increase in axial skeletal bone mass. However, there is concern that the new bone may not provide the desired increase in bone strength. Yet, NaF remains the most commonly used agent capable of stimulating bone formation in most patients (30% non-responders). But whether NaF reduces vertebral fracture rate (VFR) remains controversial. For a given treatment duration, the effect of F on bone quality appears to depend on dose: there is a marked detrimental effect on bone strength at high dose but there tends to be a beneficial effect at low dose. This biphasic NaF effect on bone strength has also been observed in fluoridated rat femurs. Unlike a study on young female rats which shows a linear dependence of cancellous bone volume (Cn-BV /TV) on NaF dose, a short-term study on young male rats, together with studies on chicks and dogs show biphasic NaF effects. Biphasic character is also observed in the effect of NaF on the packing of canine cortical bone mineral. When taken together, the animal models that show biphasic NaF effects seem to suggest that NaF at low dose improves Cn-BV/TV and bone strength and at high dose undermines them. These findings are in agreement with the clinical observations that high NaF dose does not help reduce VFR but low dose seems to help.
Increased Parathyroid Hormone Gene Expression in Secondary Hyperparathyroidism of Experimental Uremia Is Reversed by Calcimimetics (2005)

Most patients with chronic kidney disease develop secondary hyperparathyroidism with disabling systemic complications. Calcimimetic agents are effective tools in the management of secondary hyperparathyroidism, acting through allosteric modification of the calcium-sensing receptor (CaR) on the parathyroid gland (PT) to decrease parathyroid hormone (PTH) secretion and PT cell proliferation. This study showed that rats that were fed an adenine high-phosphorus diet had increased serum PTH and PTH mRNA levels at 7 and 21 d. For studying the effect of activation of the CaR by the calcimimetics R-568 on PTH gene expression, R-568 was given by gavage to uremic rats for the last 4 d of a 7-d adenine high-phosphorus diet. R-568 decreased both PTH mRNA and serum PTH levels. The effect of the calcimimetic on PTH gene expression was posttranscriptional and correlated with differences in protein-RNA binding and posttranslational modifications of the trans acting factor AUF1 in the PT. The AUF1 modifications as a result of uremia were reversed by treatment with R-568 to those of normal rats. Therefore, uremia and activation of the CaR mediated by calcimimetics modify AUF1 posttranslationally. These modifications in AUF1 correlate with changes in protein-PTH mRNA binding and PTH mRNA levels.
Quinazolinone derivatives: synthesis and binding evaluation on cholecystokinin receptors. (1996)

A series of 2-methyl-3-amino-4(H)-quinazolinone and of 2-phenyl-3-amino-4(H)-quinazolinone derivatives were synthesized and examined for their CCK receptor affinities. These compounds displayed micromolar affinities for CCK-B rather than CCK-A receptor and the obtained results confirm that the 4(3H)-quinazolinone nucleous represent a useful template for the development of selective CCK-B receptor ligands.
Rapid synthesis of gold nanorods by the combination of chemical reduction and photoirradiation processes; morphological changes depending on the growing processes (2003)

Combination of chemical reduction of tetrachloroaurate by ascorbic acid and subsequent ultraviolet photoirradiation resulted in the quick generation of gold nanorods quantitatively, with appreciable shape changes as the reaction proceeds.
Composition and Toxicity of the Inclusion of Bacillus thuringiensis subsp. israelensis (1985)

The multisegmented ovoidal inclusion of Bacillus thuringiensis subsp. israelensis was found to be composed of two structurally and biochemically distinct components. Electron microscopy of the inclusion revealed it to be composed mainly of osmiophobic or lightly stained segments crystallized in a lattice showing a repeat of approximately 4.3 nm. These light segments of the inclusions were shared by osmiophylic darkly stained segments with a crystal lattice repeat of approximately 7.8 nm. The lightly stained segments were soluble at pH 9.2 in sodium dodecyl sulfate-dithiothreitol-Tris-hydrochloride. The extracts of lightly stained segments were lytic to mammalian erythrocytes, and the precipitate obtained by lowering the pH to 5.2 was toxic to the larvae of Aedes egypti. The dark inclusion segments remaining, besides being much less toxic to larvae, were nonlytic to erythrocytes and were soluble at pH 10.5 in sodium dodecyl sulfate-dithiothreitol-Tris-hydrochloride. The light segment was composed of two major polypeptide doublets with molecular weights of 145,000 and 135,000, and 27,000 and 26,500, and the dark segments were composed of a single major polypeptide with a molecular-weight of 70,000. Hence, the inclusion of B. thuringiensis subsp. israelensis is more complex than previously reported, and we conclude that the toxin may be the polypeptide with a molecular weight of 27,000 and 26,500.
Wavelet Analysis and its Statistical Applications (2000)

In recent years there has been a considerable development in the use of wavelet methods in statistics. As a result, we are now at the stage where it is reasonable to consider such methods to be another standard tool of the applied statistician rather than a research novelty. With that in mind, this paper gives a relatively accessible introduction to standard wavelet analysis and provides a review of some common uses of wavelet methods in statistical applications. It is primarily orientated towards the general statistical audience who may be involved in analysing data where the use of wavelets might be effective, rather than to researchers who are already familiar with the field. Given that objective, we do not emphasize mathematical generality or rigour in our exposition of wavelets and we restrict our discussion to the more frequently employed wavelet methods in statistics. We provide extensive references where the ideas and concepts discussed can be followed up in greater detail and generality if required. The paper first establishes some necessary basic mathematical background and terminology relating to wavelets. It then reviews the more well-established applications of wavelets in statistics including their use in nonparametric regression, density estimation, inverse problems, changepoint problems and in some specialized aspects of time series analysis. Possible extensions to the uses of wavelets in statistics are then considered. The paper concludes with a brief reference to readily available software packages for wavelet analysis.
ATP/Mg2+-dependent binding of vincristine to the plasma membrane of multidrug-resistant K562 cells. (1988)

To study the mechanism of active drug efflux in multidrug-resistant cells, the interaction between [3H] vincristine (VCR) and plasma membrane prepared from an adriamycin (ADM)-resistant variant (K562/ADM) of human myelogenous leukemia K562 cells was examined by filtration method. [3H]VCR bound to the plasma membrane prepared from K562/ADM cells, but not from parental K562 cells, depending on the concentrations of ATP and Mg2+. Adenosine 5'-O-(3-thio)triphosphate was not effective in the binding of [3H]VCR, indicating that ATP hydrolysis is required for this binding. Dissociation constant (Kd) of VCR binding was 0.24 +/- 0.04 microM in the presence of 3 mM ATP. In the absence of ATP, specific binding of VCR to K562/ADM membrane was also observed; however, the affinity (Kd = 9.7 +/- 3.1 microM) was 40 times lower than that observed in the presence of ATP. The high affinity VCR binding to K562/ADM membrane was dependent on temperature. The bound [3H]VCR molecules were rapidly released by unlabeled VCR added to the reaction mixture at 25 degrees C. The high affinity binding of [3H]VCR to K562/ADM membrane was inhibited by VCR, vinblastine, actinomycin D, and ADM, to which K562/ADM cells exhibit cross-resistance, whereas 5-fluorouracil and camptothecin, to which K562/ADM cells are equally sensitive as K562 cells, did not inhibit the [3H]VCR binding. Furthermore, verapamil and other agents, which are known to circumvent drug resistance by inhibiting the active efflux of antitumor agents from resistant cells, could also inhibit the high affinity [3H]VCR binding. These results indicate that ATP/Mg2+-dependent high affinity VCR binding to the membrane of resistant cells closely correlates with the active drug efflux of this resistant cell line.