gebhart/patrepeval huggingface dataset:
Monolithic integration of enhancement- and depletion-mode AlGaN/GaN HEMTs for GaN digital integrated circuits (2006)

We demonstrate a novel technique for monolithic integration of enhancement and depletion-mode AlGaN/GaN HEMTs using CF/sub 4/ plasma treatment. Direct-coupled FET logic circuits such as an E/D HEMT inverter and a 17-stage ring oscillator are demonstrated in GaN system for the first time. At a supply voltage (V/sub DD/)of 1.5V, the fabricated E/D inverter shows an output logic swing of 1.25V, logic-low noise margin of 0.21V and logic-high noise margin of 0.51V. The fabricated ring oscillator shows a minimum delay of 130 ps/stage at V/sub DD/ = 3.5 V, and a minimum power-delay product of 0.113 pJ/stage at V/sub DD/ = 1 V.
A generalized processor sharing approach to flow control in integrated services networks: the multiple node case (1994)

Worst-case bounds on delay and backlog are derived for leaky bucket constrained sessions in arbitrary topology networks of generalized processor sharing (GPS) servers. The inherent flexibility of the service discipline is exploited to analyze broad classes of networks. When only a subset of the sessions are leaky bucket constrained, we give succinct per-session bounds that are independent of the behavior of the other sessions and also of the network topology. However, these bounds are only shown to hold for each session that is guaranteed a backlog clearing rate that exceeds the token arrival rate of its leaky bucket. A much broader class of networks, called consistent relative session treatment (CRST) networks is analyzed for the case in which all of the sessions are leaky bucket constrained. First, an algorithm is presented that characterizes the internal traffic in terms of average rate and burstiness, and it is shown that all CRST networks are stable. Next, a method is presented that yields bounds on session delay and backlog given this internal traffic characterization. The links of a route are treated collectively, yielding tighter bounds than those that result from adding the worst-case delays (backlogs) at each of the links in the route. The bounds on delay and backlog for each session are efficiently computed from a universal service curve, and it is shown that these bounds are achieved by "staggered" greedy regimes when an independent sessions relaxation holds. Propagation delay is also incorporated into the model. Finally, the analysis of arbitrary topology GPS networks is related to Packet GPS networks (PGPS). The PGPS scheme was first proposed by Demers, Shenker and Keshav (1991) under the name of weighted fair queueing. For small packet sizes, the behavior of the two schemes is seen to be virtually identical, and the effectiveness of PGPS in guaranteeing worst-case session delay is demonstrated under certain assignments.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Truth (2013)

Abstract According to this chapter, in an object-oriented metaphysics the truthfulness of a statement depends solely on the number of relevant agents persuaded to line-up behind it. With respect to truth, Latour is an unrepentant populist. This results in a kind of relativism. Latour criticizes other populist conceptions of truth for not being populist enough.
Organic Solvent Tolerance of Escherichia coli Is Independent of OmpF Levels in the Membrane (1999)

The organic solvent tolerance of Escherichia coli was measured under conditions in which OmpF levels were controlled by various means as follows: alteration of NaCl concentration in the medium, transformation with a stress-responsive gene (marA, robA, or soxS), or disruption of the ompF gene. It was shown that solvent tolerance of E. coli did not depend upon OmpF levels in the membrane.
Kinetics of T-cell Receptor Binding by Bivalent HLA-DR·Peptide Complexes That Activate Antigen-specific Human T-cells (2000)

Monovalent major histocompatibility complex-peptide complexes dissociate within seconds from the T-cell receptor (TCR), indicating that dimerization/multimerization may be important during early stages of T-cell activation. Soluble bivalent HLA-DR2.myelin basic protein (MBP) peptide complexes were expressed by replacing the F(ab) arms of an IgG2a antibody with HLA-DR2.MBP peptide complexes. The binding of bivalent HLA-DR2.peptide complexes to recombinant TCR was examined by surface plasmon resonance. The bivalent nature greatly enhanced TCR binding and slowed dissociation from the TCR, with a t((1)/(2)) of 2.1 to 4.6 min. Soluble bivalent HLA-DR2.MBP peptide complexes activated antigen-specific T-cells in the absence of antigen presenting cells. In contrast, soluble antibodies to the TCR.CD3 complex were ineffective, indicating that they failed to induce an active TCR dimer. TCR/CD3 antibodies induced T-cell proliferation when bound by antigen presenting cells that expressed Fc receptors. In the presence of dendritic cells, bivalent HLA-DR2. MBP peptide complexes induced T-cell activation at >100-fold lower concentrations than TCR/CD3 antibodies and were also superior to peptide or antigen. These results demonstrate that bivalent HLA-DR. peptide complexes represent effective ligands for activation of the TCR. The data support a role for TCR dimerization in early TCR signaling and kinetic proofreading.
A Meta-analysis of the Efficacy of Second-Generation Antipsychotics (2003)

Some SGAs are more efficacious than FGAs, and, therefore, SGAs are not a homogeneous group.
WHAT MAKES A CGRP2RECEPTOR? (2007)

1. Heterogeneity in the receptors for the neuropeptide calcitonin gene-related peptide (CGRP) has been apparent for nearly 20 years. This is most clearly manifested in the observation of CGRP(8-37)-sensitive and -insensitive populations of CGRP-activated receptors. The pA(2) values for CGRP(8-37) in excess of 7 are widely considered to be the result of antagonism of CGRP(1) receptors, whereas those below 7 are believed to be the consequence of antagonism of a second population of receptors, namely CGRP(2) receptors. 2. However, a multitude of pA(2) values exist for CGRP(8-37), spanning several log units, and as such no obvious clusters of values are apparent. Understanding the molecular nature of the receptors that underlie this phenomenon is likely to aid the development of selective pharmacological tools to progress our understanding of the physiology of CGRP and related peptides. Because there is active development of CGRP agonists and antagonists as therapeutics, such information would also further this pursuit. 3. The CGRP(1) receptor is pharmacologically and molecularly well defined as a heterodimer of the calcitonin receptor-like receptor (CL) and receptor activity modifying protein (RAMP) 1. The CL/RAMP1 complex is highly sensitive to CGRP(8-37). Conversely, the constituents of the CGRP(2) receptor have not been identified. In fact, there is little evidence for a distinct molecular entity that represents the CGRP(2) receptor. 4. Recent pharmacological characterization of receptors related to CGRP(1) has revealed that some of these receptors may explain CGRP(2) receptor pharmacology. Specifically, AMY(1(a)) (calcitonin receptor/RAMP1) and AM(2) (CL/RAMP3) receptors can be activated by CGRP but are relatively insensitive to CGRP(8-37). 5. This, along with other supporting data, suggests that the 'CGRP(2) receptor' that has been extensively reported in the literature may, in fact, be an amalgamation of contributions from a variety of CGRP-activated receptors. The use of appropriate combinations of agonists and antagonists, along with receptor expression studies, could allow such receptors to be separated.
Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro (1988)

Replication of the DNA genome of human adenovirus serotype 2 requires three virus-encoded proteins. Two of these proteins, the preterminal protein (pTP) and the adenovirus DNA polymerase, are transcribed from a single promoter at early times after virus infection. The mRNAs for these proteins share several exons, including one encoded near adenovirus genome coordinate 39. By using plasmids containing DNA fragments postulated to encode the various exons of pTP mRNA, the contributions of each exon to the synthesis of an active pTP have been measured. Only plasmids that contain both the open reading frame for pTP (genome coordinates 29.4 to 23.9) and the HindIII J fragment that contains the exon at genome coordinate 39 can express functional pTP.
Dynamic tuning of the IEEE 802.11 protocol to achieve a theoretical throughput limit (2000)

In wireless LANs (WLANs), the medium access control (MAC) protocol is the main element that determines the efficiency in sharing the limited communication bandwidth of the wireless channel. In this paper we focus on the efficiency of the IEEE 802.11 standard for WLANs. Specifically, we analytically derive the average size of the contention window that maximizes the throughput, hereafter theoretical throughput limit, and we show that: 1) depending on the network configuration, the standard can operate very far from the theoretical throughput limit; and 2) an appropriate tuning of the backoff algorithm can drive the IEEE 802.11 protocol close to the theoretical throughput limit. Hence we propose a distributed algorithm that enables each station to tune its backoff algorithm at run-time. The performances of the IEEE 802.11 protocol, enhanced with our algorithm, are extensively investigated by simulation. Specifically, we investigate the sensitiveness of our algorithm to some network configuration parameters (number of active stations, presence of hidden terminals). Our results indicate that the capacity of the enhanced protocol is very close to the theoretical upper bound in all the configurations analyzed.
NSC114792, a novel small molecule identified through structure-based computational database screening, selectively inhibits JAK3 (2010)

NSC114792 is a lead compound that selectively inhibits JAK3 activity. Therefore, our study suggests that this small molecule inhibitor of JAK3 can be used as a starting point to develop a new class of drugs targeting JAK3 activity, and may have therapeutic potential in various diseases that are caused by aberrant JAK3 activity.
Carbonic anhydrase in the membrane of the endoplasmic reticulum of male rat liver. (1992)

We have prepared subcellular fractions of male rat liver homogenate by the method of Lewis and Tata [Lewis, J. A. & Tata, J. R. (1973) J. Cell Sci. 23, 447-459], further purifying the membranes of the microsomal fraction by exposure to 0.01% Triton X-100 and centrifugation. We determined the purity of the fractions with marker enzymes and measured carbonic anhydrase (CA; EC 4.2.1.1) activity in intact and solubilized particulates with 18O exchange between CO2/HCO3- and water. We measured the concentration of CA by titration with a sulfonamide inhibitor, ethoxzolamide, obtaining an average value of 3.8 mumol/mg of microsomal membrane protein. The equilibrium constant for binding ethoxzolamide was 0.49 x 10(-9) M. The Km for CO2 was 1.7 mM and the turnover number was 560,000 sec-1, characterizing this as a membrane-bound, high-activity isozyme of type IV. By electron microscopy of tissue sections after staining with a cobalt precipitation technique, CA was seen in small cytoplasmic vesicles in hepatocytes and in microsomal particles and membranes. There was a sulfonamide-resistant (isozyme type III) and a sulfonamide-sensitive (isozyme type II) CA in the cytosol but none in the rapidly sedimenting endoplasmic reticulum. We conclude that there is no CA normally within the matrix of the cell endoplasmic reticulum but that the CA type III found in the microsome may have been captured from the cytosol during resealing. Thus the adult male rat hepatocyte contains CA type IV in the membrane of the endoplasmic reticulum and CA type II and CA type III in the cytoplasm.
PI3Kδ-selective and PI3Kα/δ-combinatorial inhibitors in clinical development for B-cell non-Hodgkin lymphoma (2017)

The efficacy of the prototypical phosphatidylinositol-3-kinase (PI3K) inhibitor idelalisib for the treatment of chronic lymphocytic leukemia (CLL) and indolent non-Hodgkin lymphoma (iNHL) has led to development of multiple compounds targeting this pathway. Areas Covered: We review the hypothesized therapeutic mechanisms of PI3K inhibitors, including abrogation of B cell receptor signaling, blockade of microenvironmental pro-survival signals, and enhancement of anti-tumor immunity. We examine toxicities of idelalisib, including bacterial infections (possibly secondary to drug-induced neutropenia), opportunistic infections (possibly attributable to on-target inhibition of T cell function), and organ toxicities such as transaminitis and enterocolitis (possibly autoimmune, secondary to on-target inhibition of p110δ in regulatory T cells). We evaluate PI3K inhibitors that have entered trials for the treatment of lymphoma, focusing on agents with selectivity for PI3Kα and PI3Kδ. Expert Opinion: PI3K inhibitors, particularly those that target p110δ, have robust efficacy in the treatment of CLL and iNHL. However, idelalisib has infectious and autoimmune toxicities that limit its use. Outside of trials, idelalisib should be restricted to CLL patients with progression on ibrutinib or iNHL patients with progression on two prior therapies. Whether newer PI3K inhibitors will demonstrate differentiated toxicity profiles in comparable patient populations while retaining efficacy remains to be seen.
Epigenetic Regulation of the Epithelial to Mesenchymal Transition in Lung Cancer (2017)

Lung cancer is the leading cause of cancer deaths worldwide. It is an aggressive and devastating cancer because of metastasis triggered by enhanced migration and invasion, and resistance to cytotoxic chemotherapy. The epithelial to mesenchymal transition (EMT) is a fundamental developmental process that is reactivated in wound healing and a variety of diseases including cancer where it promotes migration/invasion and metastasis, resistance to treatment, and generation and maintenance of cancer stem cells. The induction of EMT is associated with reprogramming of the epigenome. This review focuses on major mechanisms of epigenetic regulation mainly in lung cancer with recent data on EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit ), the catalytic subunit of the PRC2 (Polycomb Group PcG), that behaves as an oncogene in lung cancer associated with gene repression, non-coding RNAs and the epitranscriptome.
Bioavailability of dietary glutathione: effect on plasma concentration (1990)

Plasma glutathione (GSH) concentration in rats increased from approximately 15 to 30 microM after administration of GSH either as a liquid bolus (30 mumol) or mixed (2.5-50 mg/g) in AIN-76 semisynthetic diet. GSH concentration was maximal at 90-120 min after GSH administration and remained high for over 3 h. Administration of the amino acid precursors of GSH had little or no effect on plasma GSH values, indicating that GSH catabolism and resynthesis do not account for the increased GSH concentration seen. Inhibition of GSH synthesis and degradation by L-buthionine-[S,R]-sulfoximine and acivicin showed that the increased plasma GSH came mostly from absorption of intact GSH instead of from its metabolism. Plasma protein-bound GSH also increased after GSH administration, with a time course similar to that observed for free plasma GSH. Thus dietary GSH can be absorbed intact and results in a substantial increase in blood plasma GSH. This indicates that oral supplementation may be useful to enhance tissue availability of GSH.
Pim-1 Kinase Expression Predicts Radiation Response in Squamocellular Carcinoma of Head and Neck and Is under the Control of Epidermal Growth Factor Receptor (2009)

Pim-1 is an oncogenic serine/threonine kinase with poorly defined function in epithelial cancers. In this study, we determined 1) associations of Pim-1 expression with clinicopathological parameters including responsiveness to irradiation in squamocellular cancers of head and neck and 2) how Pim-1 expression is controlled subsequent to irradiation. Moderate to high expression of Pim-1 correlated to poor response to radiation therapy (P = .003). It is also associated to the expression of epidermal growth factor receptor (EGFR, P < .0001), which has been shown to be activated by irradiation. In radioresistant tumors, irradiation promoted nuclear translocation of Pim-1 (P < .005). When directly testing EGFR dependence of Pim-1 expression, up-regulation and nuclear translocation of Pim-1 could be induced through stimulation of EGFR with its ligands EGF or transforming growth factor alpha. Both ligand- and irradiation-induced changes in Pim-1 expression and localization could be inhibited by the monoclonal anti-EGFR antibody cetuximab and by the tyrosine kinase inhibitor gefitinib also targeting EGFR. These results suggest that irradiation-induced activation of EGFR upregulates Pim-1, and Pim-1 may be used as a novel predictive marker of radiation response in patients with squamocellular cancers of head and neck.
N-acetylcysteine Enhances Cystic Fibrosis Sputum Penetration and Airway Gene Transfer by Highly Compacted DNA Nanoparticles (2011)

For effective airway gene therapy of cystic fibrosis (CF), inhaled gene carriers must first penetrate the hyperviscoelastic sputum covering the epithelium. Whether clinically studied gene carriers can penetrate CF sputum remains unknown. Here, we measured the diffusion of a clinically tested nonviral gene carrier, composed of poly-l-lysine conjugated with a 10 kDa polyethylene glycol segment (CK(30)PEG(10k)). We found that CK(30)PEG(10k)/DNA nanoparticles were trapped in CF sputum. To improve gene carrier diffusion across sputum, we tested adjuvant regimens consisting of N-acetylcysteine (NAC), recombinant human DNase (rhDNase) or NAC together with rhDNase. While rhDNase alone did not enhance gene carrier diffusion, NAC and NAC + rhDNase increased average effective diffusivities by 6-fold and 13-fold, respectively, leading to markedly greater fractions of gene carriers that may penetrate sputum layers. We further tested the adjuvant effects of NAC in the airways of mice with Pseudomonas aeruginosa lipopolysaccharide (LPS)-induced mucus hypersecretion. Intranasal dosing of NAC prior to CK(30)PEG(10k)/DNA nanoparticles enhanced gene expression by up to ~12-fold compared to saline control, reaching levels observed in the lungs of mice without LPS challenge. Our findings suggest that a promising synthetic nanoparticle gene carrier may transfer genes substantially more effectively to lungs of CF patients if administered following adjuvant mucolytic therapy with NAC or NAC + rhDNase.
3D printed flow plates for the electrolysis of water: an economic and adaptable approach to device manufacture (2014)

The electrolysis of water is considered a promising route to the production of hydrogen from renewable energy sources. Electrolysers based on proton exchange membranes (PEMs) have a number of advantages including high current density, high product gas purity and the ability to operate at high pressure. Despite these advantages the high cost of such devices is an impediment to their widespread deployment. A principal factor in this cost are the materials and machining of flow plates for distribution of the liquid reagents and gaseous products in the electrochemical cell. We demonstrate the production and operation of a PEM electrolyser constructed from silver coated 3D printed components fabricated from polypropylene. This approach allows construction of light weight, low cost electrolysers and the rapid prototyping of flow field design. Furthermore we provide data on the operation of this electrolyser wherein we show that performance is excellent for a first generation device in terms of overall efficiency, internal resistances and current–voltage response. This development opens the door to the fabrication of light weight and cheap electrolysers as well as related electrochemical devices such as flow batteries and fuel cells.
From nanodevices to nanosystems: The N3XT information technology (2015)

Next-generation information technologies will process unprecedented amounts of loosely-structured data, including streaming video and audio, natural languages, real-time sensor readings, and contextual environments. These newly available data far exceed the processing capacity of existing computing architectures.
Comparative columnar porous etching studies on n‐type 6H SiC crystalline faces (2008)

Abstract Comparative photo‐electrochemical etchings have been performed on the Si‐face, C‐face and a‐face of n‐type 6H SiC. Two kinds of columnar pores are developed: nano‐columnar (∼20 nm diameter) and macro‐columnar (∼one µm diameter). The time dependences of the current densities during the etchings are discussed. Our results show that the nano‐columnar pore formation is associated with the anisotropic electrochemical oxidation reaction rate on the different crystal faces. Our experiments show that, in the case of 6H SiC, the C‐face should be used for nano‐columnar pore formation. The electrochemical oxidation rate increases with the surface C atom density of SiC. (© 2008 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Hospitalist Care and Length of Stay in Patients Requiring Complex Discharge Planning and Close Clinical Monitoring (2007)

Teaching hospitalist care was associated with shorter LOS in patients requiring close clinical monitoring and complex discharge planning, without adversely affecting readmission or mortality rates.
Functional human corneal endothelial cell sheets harvested from temperature‐responsive culture surfaces (2005)

This study reports a new method for fabricating bioengineered human corneal endothelial cell sheets suitable for ocular surgery and repair. We have initially cultured human corneal endothelial cells on type IV collagen-coated dishes and, after several passages, expanded cells were then seeded onto novel temperature-responsive culture dishes. Four weeks after reaching confluence, these cultured endothelial cells were harvested as intact monolayer cell sheets by simple temperature reduction without enzymatic treatment. Scanning electron microscopy indicated that these cells were primarily hexagonal with numerous microvilli and cilia, similar to the native corneal endothelium. The Na+, K+-ATPase pump sites were located at the cell borders as in vivo. Moreover, cell densities and numbers of pump sites were identical to those of in vivo human corneal endothelium under optimized conditions. A 3H-ouabain binding analysis demonstrated a linear proportionality for cell pump density between confluent cell densities of 575 cells/mm2 and 3070 cells/mm2. We also confirmed Na+, K+-ATPase activity in the sheets in vitro. Xenograft transplantation results showed that the fabricated sheets retain their function of maintaining proper stromal hydration in vivo. We have established a regimen to culture and proliferate human corneal endothelial cells and fabricate endothelial sheets ex vivo morphologically and functionally similar to the native corneal endothelium. Our results support the value of harvested cell sheets for clinical applications in ocular reconstructive surgery in patients with ocular endothelial decompensation.