gebhart/patrepeval huggingface dataset:
Fabrication, characterization and evaluation of bioceramic hollow microspheres used as microcarriers for 3-D bone tissue formation in rotating bioreactors (1999)

Novel bioactive ceramic hollow microspheres with an apparent density in the range 0.8-1.0 g cm(-3) have been developed as microcarriers for 3-D bone tissue formation in rotating-wall vessels (RWV). Hollow ceramic microspheres with a composition of 58-72% SiO2, 28-42% Al2O3 (wt%) and an apparent density 0.8-1.0 g cm(-3) were pretreated in 1.0 N NaOH for 2 h before being coated with synthesized calcium hydroxyapatite (HA) particulate sol. The HA-coated hollow microspheres were sintered for 1 h at 600, 800 and 1000 degrees C. SEM analysis revealed that the grain size and pore size of the calcium phosphate coating increased with the sintering temperature. FTIR analysis showed that crystalline calcium hydroxyapatite was present in the coatings sintered at 600 and 800 degrees C. When sintered at 1000 degrees C, the coating consisted of alpha-tricalcium phosphate. All the coatings adhered well, independent of sintering temperature. The trajectory analysis revealed that the hollow microsphere remained suspended in a rotating-wall vessel (RWV), and experienced a low shear stress (approximately 0.6 dyn cm(-2)). Cell culture studies using rat bone marrow stromal cells and osteosarcoma cells (ROS 17/2.8) showed that the cells attached to and formed 3-D aggregates with the hollow microspheres in a RWV. Extracellular matrix was observed in the aggregates. These data suggest that these hollow bioactive ceramic microspheres can be used as microcarriers for 3-D bone tissue formation in vitro, as well as for the study of the effects of microgravity on bone cell functions.
Male sterility in soybean and maize: developmental comparisons (1992)

Sexual reproduction in angiosperms is a complex process that includes a portion of the sporophytic (spore-producing) generation and all of the gametophytic (gamete-producing) generation. The gametophytic generation includes the developmental stages from the end of meiosis to fertilization. For normal reproduction, coordination of both female and male reproductive ontogenies must occur. An abnormality anywhere in this process may lead to sterility.
Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma (2007)

Excessive airway obstruction is the cause of symptoms and abnormal lung function in asthma. As airway smooth muscle (ASM) is the effecter controlling airway calibre, it is suspected that dysfunction of ASM contributes to the pathophysiology of asthma. However, the precise role of ASM in the series of events leading to asthmatic symptoms is not clear. It is not certain whether, in asthma, there is a change in the intrinsic properties of ASM, a change in the structure and mechanical properties of the noncontractile components of the airway wall, or a change in the interdependence of the airway wall with the surrounding lung parenchyma. All these potential changes could result from acute or chronic airway inflammation and associated tissue repair and remodelling. Anti-inflammatory therapy, however, does not "cure" asthma, and airway hyperresponsiveness can persist in asthmatics, even in the absence of airway inflammation. This is perhaps because the therapy does not directly address a fundamental abnormality of asthma, that of exaggerated airway narrowing due to excessive shortening of ASM. In the present study, a central role for airway smooth muscle in the pathogenesis of airway hyperresponsiveness in asthma is explored.
hERG channel trafficking: novel targets in drug-induced long QT syndrome (2007)

The cardiac potassium channel hERG (human ether-a-go-go-related gene) encodes the alpha-subunit of the rapid delayed rectifier current I(Kr) in the heart, which contributes to terminal repolarization in human cardiomyocytes. Direct block of hERG/I(Kr) channels by a large number of therapeutic compounds produces acLQTS [acquired LQTS (long QT syndrome)] characterized by drug-induced QT prolongation and torsades de pointes arrhythmias. The cardiotoxicity associated with unintended hERG block has prompted pharmaceutical companies to screen developmental compounds for hERG blockade and made hERG a major target in drug safety programmes. More recently, a novel form of acLQTS has been discovered that may go undetected in most conventional safety assays. Several therapeutic compounds have been identified that reduce hERG/I(Kr) currents not by direct block but by inhibition of hERG/I(Kr) trafficking to the cell surface. Important examples are antineoplastic Hsp90 (heat-shock protein 90) inhibitors such as (i) geldanamycin, (ii) the leukaemia drug arsenic trioxide, (iii) the antiprotozoical pentamidine, (iv) probucol, a cholesterol-lowering drug, and (v) fluoxetine, a widely used antidepressant. Increased awareness of drug-induced hERG trafficking defects will help to further reduce the potentially lethal adverse cardiac events associated with acLQTS.
Isolation, sequencing and overexpression of the gene encoding theθsubunit of DNA polymerase III holoenzyme (1993)

The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.
Severe hypovitaminosis C occurring as the result of adoptive immunotherapy with high-dose interleukin 2 and lymphokine-activated killer cells. (1987)

Adoptive immunotherapy of human cancer was investigated in our institution as part of a National Cancer Institute extramural group study. This treatment, for patients with metastatic malignant melanoma, hypernephroma, and colon carcinoma, consisted of three phases: (a) 5 days of i.v. high-dose (10(5) units/kg every 8 h) interleukin 2, (b) 6 1/2 days of rest plus leukapheresis; and (c) 4 days of high-dose interleukin 2 plus three infusions of autologous lymphokine-activated killer cells. Toxicities included fever, chills, tachycardia, hypotension, vomiting, diarrhea, and fluid retention. Ascorbic acid is known to be important to cell-mediated immunity, and it has been reported to be depleted during physiologically stressful events. Therefore, we determined plasma ascorbic acid levels in patients (n = 11) before adoptive immunotherapy and before and after Phases 1, 2, and 3 of treatment. Patients entering the trial were not malnourished. Mean plasma ascorbic acid levels were normal (0.64 +/- 0.25 mg/dl) before therapy. Mean levels dropped by 80% after the first phase of treatment with high-dose interleukin 2 alone (0.13 +/- 0.08 mg/dl). Mean plasma ascorbic acid levels remained severely depleted (0.08 to 0.13 mg/dl) throughout the remainder of the treatment, becoming undetectable (less than 0.05 mg/dl) in eight of 11 patients during this time. Values obtained from 24-h urine collections on two of two patients indicated that ascorbate was not excreted in the urine. Plasma ascorbic acid normalized in three of three patients tested 1 mo after the completion of treatment. Unlike the results for ascorbic acid, blood pantothenate and plasma vitamin E remained within normal limits in all 11 patients throughout the phases of therapy. Responders (n = 3) differed from nonresponders (n = 8) in that plasma ascorbate levels in the former recovered to at least 0.1 mg/dl (frank clinical scurvy) during Phases 2 and 3, whereas levels in the latter fell below this level.
A Vector-Perturbation Technique for Near-Capacity Multiantenna Multiuser Communication— Part II: Perturbation (2005)

Recent theoretical results describing the sum-capacity when using multiple antennas to communicate with multiple users in a known rich scattering environment have not yet been followed with practical transmission schemes that achieve this capacity. We introduce a simple encoding algorithm that achieves near-capacity at sum-rates of tens of bits/channel use. The algorithm is a variation on channel inversion that regularizes the inverse and uses a "sphere encoder" to perturb the data to reduce the energy of the transmitted signal. The paper is comprised of two parts. In this second part, we show that, after the regularization of the channel inverse introduced in the first part, a certain perturbation of the data using a "sphere encoder" can be chosen to further reduce the energy of the transmitted signal. The performance difference with and without this perturbation is shown to be dramatic. With the perturbation, we achieve excellent performance at all signal-to-noise ratios. The results of both uncoded and turbo-coded simulations are presented.
Localization of focal adhesion kinase isoforms in cells of the central nervous system (2003)

Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase which in non-neuronal cells is localized to focal adhesions, where it participates to adhesion-dependent intracellular signalling. FAK is highly expressed in the central nervous system both during development and in the adult. FAK(+), a splice isoform of FAK selectively enriched in neurons, contains a three-amino acid insertion in the carboxy-terminal sequence responsible for the localization of FAK to focal adhesions. Enhanced green fluorescent protein-tagged constructs were used to study the targeting of FAK and FAK(+) in neuronal and non-neuronal cells of the central nervous system. In transfected non-neuronal cells, both fusion proteins colocalized with vinculin in focal contacts. When expressed in hippocampal neurons in culture, both chimeras were locally concentrated in the growth cone, where they overlapped with F-actin enrichments but not with vinculin. In the growth cone of living neurons, the FAK(+) chimera showed a dynamic relocalization to membrane ruffles and to the tips of the membrane protrusions induced by cytochalasin D treatment, indicating a dependence of FAK distribution on F-actin organization. Since virtually identical patterns of distribution were found for FAK and FAK(+) chimeras, it follows that the additional insertion in FAK(+) is not responsible for the localization of the kinase. Finally, we showed that the carboxy-terminal domain of both FAK and FAK(+) is sufficient to mediate the localization of the proteins to focal adhesions in non-neuronal cells and to maintain their correct intracellular targeting in neurons.
The influence of Pt particle size on the surface oxidation of titania supported platinum (2009)

A range of reduced titania (TiO(x)) supported platinum electrocatalysts have been synthesised using physical vapour deposition on arrays of electrodes. Surfaces with equivalent thicknesses of platinum in the range 0.2-2.5 nm on a uniform layer of TiO(x) have been synthesised on 10 x 10 arrays. The arrays have been used to study the surface redox chemistry of the supported platinum as well as the oxidation of a monolayer of carbon monoxide on the platinum. It is shown that below an equivalent thickness of 0.8 nm, there is a positive shift in the potential for the oxidation of the platinum surface and a negative shift for the reduction of the oxide with decrease in the platinum loading. These shifts show that it is the kinetics of the platinum/platinum oxide couple that change with platinum loading; the couple becomes increasingly irreversible with decreasing loading. The peak potential for the oxidation of the monolayer of carbon monoxide also shifts positive and broadens with decreasing platinum loading; these trends are again particularly marked below an equivalent thickness of 0.8 nm while below 0.4 nm no CO oxidation peak is observed although it could be confirmed that CO is adsorbed on such surfaces. Again, these changes with platinum loading are associated with the irreversibility of the platinum/platinum oxide couple. At low equivalent thicknesses, it is impossible to form the oxidised platinum species within the carbon monoxide monolayer essential to the commencement of oxidation of the CO monolayer.
Opponent modeling in poker (1998)

Poker is an interesting test-bed for artificial intelligence research. It is a game of imperfect knowledge, where multiple competing agents must deal with risk management, agent modeling, unreliable information and deception, much like decision-making applications in the real world. Agent modeling is one of the most difficult problems in decision-making applications and in poker it is essential to achieving high performance. This paper describes and evaluates Loki, a poker program capable of observing its opponents, constructing opponent models and dynamically adapting its play to best exploit patterns in the opponents&amp;apos; play. Introduction The artificial intelligence community has recently benefited from the tremendous publicity generated by the development of chess, checkers and Othello programs that are capable of defeating the best human players. However, there is an important difference between these board games and popular card games like bridge and poker. In the board games, pla...
Fluorescent Nanoparticle Uptake for Brain Tumor Visualization (2006)

Accurate delineation of tumor margins is vital to the successful surgical resection of brain tumors. We have previously developed a multimodal nanoparticle CLIO-Cy5.5, which is detectable by both magnetic resonance imaging and fluorescence, to assist in intraoperatively visualizing tumor boundaries. Here we examined the accuracy of tumor margin determination of orthotopic tumors implanted in hosts with differing immune responses to the tumor. Using a nonuser-based signal intensity method applied to fluorescent micrographs of 9L gliosarcoma green fluorescent protein (GFP) tumors, mean overestimations of 2 and 24 microm were obtained using Cy5.5 fluorescence, compared to the true tumor margin determined by GFP fluorescence, in nude mice and rats, respectively. To resolve which cells internalized the nanoparticle and to quantitate degree of uptake, tumors were disaggregated and cells were analyzed by flow cytometry and fluorescence microscopy. Nanoparticle uptake was seen in both CD11b+ cells (representing activated microglia and macrophages) and tumor cells in both animal models by both methods. CD11b+ cells were predominantly found at the tumor margin in both hosts, but were more pronounced at the margin in the rat model. Additional metastatic (CT26 colon) and primary (Gli36 glioma) brain tumor models likewise demonstrated that the nanoparticle was internalized both by tumor cells and by host cells. Together, these observations suggest that fluorescent nanoparticles provide an accurate method of tumor margin estimation based on a combination of tumor cell and host cell uptake for primary and metastatic tumors in animal model systems and offer potential for clinical translation.
Pharmacokinetics and dosimetry of an alpha-particle emitter labeled antibody: 213Bi-HuM195 (anti-CD33) in patients with leukemia. (1999)

This study shows that patient imaging of 213Bi, an alpha-particle emitter, labeled to HuM195 is possible and may be used to derive pharmacokinetics and dosimetry. The absorbed dose ratio between marrow, liver and spleen volumes and the whole body for 213Bi-HuM195 is 1000-fold greater than that commonly observed with beta-emitting radionuclides used for radioimmunotherapy.
Chemical Modifications of Antisense Morpholino Oligomers Enhance Their Efficacy against Ebola Virus Infection (2009)

Phosphorodiamidate morpholino oligomers (PMOs) are uncharged nucleic acid-like molecules designed to inactivate the expression of specific genes via the antisense-based steric hindrance of mRNA translation. PMOs have been successful at knocking out viral gene expression and replication in the case of acute viral infections in animal models and have been well tolerated in human clinical trials. We propose that antisense PMOs represent a promising class of therapeutic agents that may be useful for combating filoviral infections. We have previously shown that mice treated with a PMO whose sequence is complementary to a region spanning the start codon of VP24 mRNA were protected against lethal Ebola virus challenge. In the present study, we report on the abilities of two additional VP24-specific PMOs to reduce the cell-free translation of a VP24 reporter, to inhibit the in vitro replication of Ebola virus, and to protect mice against lethal challenge when the PMOs are delivered prior to infection. Additionally, structure-activity relationship evaluations were conducted to assess the enhancement of antiviral efficacy associated with PMO chemical modifications that included conjugation with peptides of various lengths and compositions, positioning of conjugated peptides to either the 5' or the 3' terminus, and the conferring of charge modifications by the addition of piperazine moieties. Conjugation with arginine-rich peptides greatly enhanced the antiviral efficacy of VP24-specific PMOs in infected cells and mice during lethal Ebola virus challenge.
Optical Properties of Inverse Opal Photonic Crystals (2002)

Colloidal crystal-templating methods have been used to prepare inverse opal photonic crystals of silica, mercaptopropyl-functionalized silica, titania, and zirconia. Ordered arrays of uniformly sized polymer spheres were infiltrated with fluid precursors capable of condensation or crystallization. After solidification of the material in the void spaces between the spheres, the polymer templates were removed by calcination or solvent extraction, leaving inverse replicas of the template arrays. By carefully controlling the synthetic procedures, gram-scale quantities of powdered macroporous materials exhibiting photonic crystal properties were obtained. For materials with crystalline walls (titania and zirconia), this required minimization of the size of the nanocrystalline grains. Because the periodicity introduced into the wall structure by the colloidal crystal templates was on the order of optical wavelengths, Bragg diffractions from the planes produced photonic stop bands in the visible spectra of these materials. The stop bands were manifested as brightly colored reflections and an optical filtering behavior of the materials. A crystallographic indexing of the optical spectrum of a polycrystalline inverse opal confirmed the fcc ordering of the pores. The optical properties of these materials were modified in predictable manners by numerous methods, including tailoring the pore size, filling the pores with fluids of various refractive indices, and changing the compositions of the solid material. The wavelengths of the colorful reflections (stop bands) were found to be proportional to the pore size and to vary linearly with the refractive index of the fluid filling the pores. The physical and synthetic modifications reported here allowed for the preparation of powders with optical reflections and bright colors spanning the entire visible spectrum.
How good are fuzzy If-Then classifiers? (2000)

This paper gives some known theoretical results about fuzzy rule-based classifiers and offers a few new ones. The ability of Takagi-Sugeno-Kang (TSK) fuzzy classifiers to match exactly and to approximate classification boundaries is discussed. The lemma by Klawonn and Klement about the exact match of a classification boundary in R (2) is extended from monotonous to arbitrary functions. Equivalence between fuzzy rule-based and nonfuzzy classifiers (1-nn and Parzen) is outlined. We specify the conditions under which a class of fuzzy TSK classifiers turn into lookup tables. It is shown that if the rule base consists of all possible rules (all combinations of linguistic labels on the input features), the fuzzy TSK model is a lookup classifier with hyperbox cells, regardless of the type (shape) of the membership functions used. The question "why fuzzy?" is addressed in the light of these results.
Fatty Acid Metabolic Defects and Right Ventricular Lipotoxicity in Human Pulmonary Arterial Hypertension (2016)

Abnormalities in FA metabolism can be detected in the blood and myocardium in human PAH and are associated with in vivo cardiac steatosis and lipotoxicity. Murine data suggest that lipotoxicity may arise from reduction in FA oxidation.
Hyperoxia-induced apoptosis of AEC II in premature rats by inhibiting expression of focal adhesion kinase (2009)

Objective To explore the role of focal adhesion kinase (FAK) in hyperoxia-apopto- sis of type Ⅱ alveolar epithelial cells (AEC Ⅱ s) of premature rats. Methods AEC Ⅱ from prema- ture rat lungs were cultured and randomly assigned to air group and hyperoxia group. After exposed to hyperoxia for 6, 12, 24 and 48 h, apoptosis rate of AEC Ⅱ were analyzed by flow cytometry with an- nexin-Ⅴ/propidium iodine (PI) double staining. FAK mRNA and FAK and fAK-Tyr397 peptide were detected by RT-PCR and Western blot, respectively. Results Positive cells of Annexin-Ⅴ+ / PI- in AEC Ⅱ after 6,12,24 and 48 h of hyperoxia exposure were significantly decreased, and the maximal apoptosis rate of AEC Ⅱ (stained of Annexin-Ⅴ+ / PI- ) was found in the hyperoxia group at 12 h (23.83%±4.43%). Compared to the air group, the expression of FAK mRNA and of FAK de- creased markedly and progressively in hyperoxia groups at 12, 24 and 48 h(P<0. 05). Conclusions Decreased expression of FAK induced by hyperoxia is likely to contribute to the apoptosis and neco-z sis of AEC Ⅱ. 

Key words: 
Focal adhesion protein-tyrosine kinases;  Pulmonary alveoli;  Epithelial cells;  Apop- tosis;  Hyperoxia;  Infant, premature
Making ends meet in genetic analysis using padlock probes (2002)

Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single-nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling-circle replication, as well as to sort reaction products on tag-arrays. We argue that the probes have the potential to render high-throughput genetic analyses precise and affordable.
Fecal Microbiota Transplantation: Current Applications, Effectiveness, and Future Perspectives (2016)

Fecal microbiota transplantation (FMT) is the infusion of liquid filtrate feces from a healthy donor into the gut of a recipient to cure a specific disease. A fecal suspension can be administered by nasogastric or nasoduodenal tube, colonoscope, enema, or capsule. The high success rate and safety in the short term reported for recurrent Clostridium difficile infection has elevated FMT as an emerging treatment for a wide range of disorders, including Parkinson's disease, fibromyalgia, chronic fatigue syndrome, myoclonus dystopia, multiple sclerosis, obesity, insulin resistance, metabolic syndrome, and autism. There are many unanswered questions regarding FMT, including donor selection and screening, standardized protocols, long-term safety, and regulatory issues. This article reviews the efficacy and safety of FMT used in treating a variety of diseases, methodology, criteria for donor selection and screening, and various concerns regarding FMT.
Expanding the TRANSFAC database towards an expert system of regulatory molecular mechanisms (1999)

TRANSFAC is a database on transcription factors, their genomic binding sites and DNA-binding profiles. In addition to being updated and extended by new features, it has been complemented now by a series of additional database modules. Among them, modules which provide data about signal transduction pathways (TRANSPATH) or about cell types/organs/developmental stages (CYTOMER) are available as well as an updated version of the previously described COMPEL database. The databases are available on the WWW at http://transfac.gbf.de/