gebhart/patrepeval huggingface dataset:
Google aims for quantum computing supremacy [News] (2017)

Quantum computers have long held the promise of performing certain calculations that are impossible- or at least, entirely impractical-for even the most powerful conventional computers to perform. Now, researchers at a Google laboratory in Goleta, Calif., may finally be on the cusp of proving it, using the same kinds of quantum bits, or qubits, that one day could make up largescale quantum machines. By the end of this year, the team aims to increase the number of superconducting qubits it builds on integrated circuits to create a 7-by-7 array. With this quantum IC, the Google researchers aim to perform operations at the edge of what's possible with even the best supercomputers, and so demonstrate "quantum supremacy".
Immunogenicity of umbilical cord tissue–derived cells (2007)

Umbilical cord tissue provides a unique source of cells with potential for tissue repair. Umbilical cord tissue-derived cells (UTCs) are MHC class I (MHCI) dull and negative for MHC class II (MHCII), but can be activated to increase MHCI and to express MHCII with IFN-gamma stimulation. Mesenchymal stem cells with similar characteristics have been inferred to be nonimmunogenic; however, in most cases, immunogenicity was not directly assessed. Using UTC from Massachusetts General Hospital MHC-defined miniature swine, we assessed immunogenicity across a full MHC barrier. Immunogenicity was assessed by in vitro assays including mixed lymphocyte reaction (MLR) and flow cytometry to detect serum alloantibody. A single injection of MHC-mismatched unactivated UTCs did not induce a detectable immune response. When injected in an inflamed region, injected repeatedly in the same region or stimulated with IFN-gamma prior to injection, UTCs were immunogenic. As clinical cellular repair strategies may involve injection of allogeneic cells into inflamed regions of damaged tissue or repeated doses of cells to achieve the desired benefit, our results on the immunogenicity of these cells in these circumstances may have important implications for optimal success and functional improvement for this cellular treatment strategy for diseased tissues.
Multi-Carrier CDMA in Indoor Wireless Radio Networks (1994)

This paper examines a novel digital modulation/multiple access technique called Multi-Carrier Code Division Multiple Access (MC-CDMA) where each data symbol is transmitted at multiple narrowband subcarriers. Each subcarrier is encoded with a phase offset of 0 or p based on a spreading code. Analytical results are presented on the performance of this modulation scheme in an indoor wireless multipath radio channel. Introduction This paper examines the performance of a new spread spectrum transmission method called &amp;quot;MCCDMA &amp;quot; in an indoor wireless environment. MC-CDMA may be a suitable modulation technique in the indoor environment where the dispersive character of indoor propagation [1] allows for the exploitation of this technique. With MC-CDMA, each data symbol is transmitted over N narrowband subcarriers where each subcarrier is encoded with a 0 or p phase offset. If the number of and spacing between subcarriers is appropriately chosen, it is unlikely that all of the subcarriers will...
Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses. (1990)

We describe here a monoclonal antibody (H398) that immunoprecipitates a human 60-kD tumor necrosis factor (TNF) membrane receptor (p60) and competes with TNF binding to p60 but not to p85 TNF receptors. Despite partial inhibition of TNF binding capacity of cells coexpressing both TNF receptor molecules, H398 uniformly and completely inhibits very distinct TNF responses on a variety of cell lines. These data suggest a limited structural heterogeneity in those components actually contributing to TNF responsiveness and identify p60 as a common receptor molecule essential for TNF signal transduction. As H398 is a highly effective TNF antagonist in vitro, it might be useful as a therapeutic agent in the treatment of TNF-mediated acute toxicity.
Human Antibody Responses to HIV Type 1 Glycoprotein 41 Cloned in Phage Display Libraries Suggest Three Major Epitopes Are Recognized and Give Evidence for Conserved Antibody Motifs in Antigen Binding (1996)

A large panel of human Fab fragments against the gp41 subunit of the HIV-1 envelope glycoprotein was isolated by panning six phage-displayed antibody libraries against recombinant gp41. The libraries were prepared from HIV-1-seropositive donors. Twenty-three Fabs recognizing conformation-dependent determinants on gp41 were isolated. Further selection of libraries against (1) gp41 ligated with Fabs from the initial selection and against (2) a recombinant gp41-containing gp140 protein yielded five additional Fabs. Competition of members of the Fab panel with one another and with previously described antibodies revealed a series of overlapping specificities that were conveniently grouped into three major epitope clusters. The majority of Fabs recognized epitopes involving residues 649-668 (previously known as the cluster II region), numbered using the Los Alamos LAI sequence. A second set of Fabs reacted with an epitope involving residues 584-609 (known as the cluster I region). Another set of Fabs appeared to recognize a third conformational epitope that has been termed the cluster III region. This third Fab epitope group demonstrated some overlap with both clusters I and II in binding assays. None of the Fabs neutralized HIV-1 laboratory strains at biologically significant concentrations. This tends to support the opinion that a vaccine based on the gp41 molecule has the drawback that neutralizing epitopes of gp41 are rare and/or unfavorably presented to the immune system. Analysis of heavy chain sequences revealed common CDR3 motif sequences in several antibodies, which appears to be an interesting consequence of a persistent immune response to conserved antigen structures.
Non‐replicating vaccinia vector efficiently expresses bacteriophage T7 RNA polymerase (1995)

Modified vaccinia virus Ankara (MVA), a host range restricted and highly attenuated vaccinia virus strain, is unable to multiply in human and most other mammalian cell lines. Since viral gene expression is unimpaired in non‐permissive cells recombinant MVA viruses are efficient as well as exceptionally safe expression vectors. We constructed a recombinant MVA that expresses the bacteriophage T7 RNA polymerase and tested its usefulness for transient expression of recombinant genes under the control of a T7 promoter. Using the chloramphenicol acetyltransferase (CAT) gene as a reporter gene, infection with MVA‐T7pol allowed efficient synthesis of recombinant enzyme in mammalian cells. Despite the severe host restriction of MVA, enzyme activities induced by infection with MVA‐T7pol were similar to those determined after infection with a replication‐competent vaccinia‐T7pol recombinant virus. Thus, MVA‐T7pol may be used as a novel vaccinia vector to achieve T7 RNA polymerase‐specific recombinant gene expression in the absence of productive vaccinia virus replication.
Neural networks for solving systems of linear equations. II. Minimax and least absolute value problems (1992)

For pt.I see ibid., vol.39, no.2, p.124-38 (1992). The minimax (L/sub infinity /- or Chebyshev norm) and the least absolute value (L/sub 1/-norm) optimization criteria for linear parameter estimation problems are reformulated as constrained minimization problems. For these problems appropriate energy (Lyapunov) functions are constructed which enable the problems to be mapped into systems of nonlinear ordinary differential equations. On the basis of these systems of equations, analog neuronlike network architectures are proposed and their properties are discussed. The proposed circuit structures exhibit a high degree of modularity, and in most cases a relatively small number of basic building blocks (processing units) are required to implement effective and powerful optimization algorithms. The validity and performance of the architectures are illustrated by extensive computer simulations and CMOS implementations of a general-purpose network architecture are considered.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Repurposing a novel parathyroid hormone analogue to treat hypoparathyroidism (2017)

This article is part of a themed section on Inventing New Therapies Without Reinventing the Wheel: The Power of Drug Repurposing. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.2/issuetoc.
Phosphate Removal by Resin Hemoperfusion Efficacy and Biocompatibility of a New Exchange Resin (1992)

A new coated anionic exchange resin for blood purification specifically designed to remove phosphates was experimentally employed in animals. 3 pigs, in which uremia had been surgically induced, underwent 6 extracorporeal hemoperfusion sessions (2 per pig) with a cartridge containing 100 gr of resin. The phosphate clearance proved satisfactory, values being 120 ml/min after 10' and around 80 ml/min after 2 hours. The biocompatibility of the resin and of the coating membrane was satisfactory. The negligible variation in pH and plasma bicarbonate during all sessions confirmed the low absorption by the tested resin of other blood anions competing with phosphate.
Release of Free Fatty Acids from Subcutaneous Adipose Tissue in Dogs Following Sympathetic Nerve Stimulation1 (1966)

Abstract A technique suitable for quantitative measurements of circulatory and metabolic processes in subcutaneous adipose tissue in vivo is described. Electrical stimulation of sympathetic nerves to the adipose tissue induces a net release of FFA as well as vasoconstriction. A maximal release rate of FFA seems to be obtained with a stimulus frequency of about 3/sec. The vasoconstrictor reaction following sympathetic nerve stimulation supresses the release rate of FFA. This is especially true following stimulation with higher frequencies of 5/sec, or more. Provided the stimulation frequencies are within the physiological range the stimulation period must be 2 min, or more, in order to produce a rise in the release rate of FFA. The data indicate that sympathetic nerves to subcutaneous adipose tissue have the ability to mobilize energy for the organism within a few minutes.
Homogeneous one-dimensional optical lattice generation using a digital micromirror device-based high-precision beam shaper (2012)

A homogeneous one-dimensional optical lattice is demonstrated by using a high-precision beam shaper based on a digital micromirror device (DMD) with an imaging system containing a pinhole low-pass filter (LPF). This system is capable of producing a high-quality flattop beam profile to form a standing-wave optical lattice with a 50×50 μm2 flattop region. The periodic potential generated by the optical lattice confines ultracold atoms in Bose-Einstein condensate experiments. We conducted beam shaping tests at several wavelengths by implementing various coherent and incoherent light sources in the visible and infrared wavelength ranges. Experiments produced flattop and other well-controlled beam profiles with 0.2% to 0.26% root-mean-square (RMS) error after applying a digital LPF and nearly flat phase. Several concerns for the system design are presented. First, the energy requirement was determined by power conversion analysis and DMD diffraction efficiency simulation. In addition, a LabVIEW program was written to accelerate the speed of the iterative process for beam profile refinement. Finally, various camera calibrations improved the measurement accuracy. We achieved a 1.25% RMS error flattop beam with diameter of 70.4 μm at the atoms' plane. Other beam profile measurements in different diagnostic planes demonstrated a good intensity uniformity of the optical lattice.
Prevalence and Clinical Significance of Epileptiform EEG Discharges in a Large Memory Clinic Cohort (2010)

In a memory clinic cohort, epileptiform discharges are rare, nonspecific and indicate a moderate risk of first-ever seizures in demented patients. These findings should be taken into account when routinely evaluating memory clinic patients with EEG.
Synthesis of Extremely Large Mesoporous Activated Carbon and Its Unique Adsorption for Giant Molecules (1996)

The steam invigoration of pitches (softening points 85 and 280 °C) homogenized with 1−3 wt % of organo rare-earth metal complexes such as Ln(C5H5)3 or Ln(acac) (Ln = Y, Yb) at 930 °C provided activated carbons with an extremely high mesopore ratio, >70%. The resulted activated carbon selectively adsorbs giant molecules such as vitamin B12, blue acid 90 dye, dextran, nystatin, and humic acid, reflecting their large mesopore volumes. To understand what kind of carbon skeleton in pitch is suited for generation of high mesopore ratio, the steam invigoration of a series of condensed polynuclear aromatics (COPNA) resins prepared from naphthalene, anthracene, phenanthrene, pyrene, or perylene and p-xylene-α,α‘-diol were conducted in the presence of rare-earth metal complexes. As a result, COPNA resins containing phenanthrene, perylene, and pyrene generated large mesopore volume.
Global Profiling of Proteolysis during Rupture of Plasmodium falciparum from the Host Erythrocyte (2010)

The obligate intracellular parasite pathogen Plasmodium falciparum is the causative agent of malaria, a disease that results in nearly one million deaths per year. A key step in disease pathology in the human host is the parasite-mediated rupture of red blood cells, a process that requires extensive proteolysis of a number of host and parasite proteins. However, only a relatively small number of specific proteolytic processing events have been characterized. Here we describe the application of the Protein Topography and Migration Analysis Platform (PROTOMAP) (Dix, M. M., Simon, G. M., and Cravatt, B. F. (2008) Global mapping of the topography and magnitude of proteolytic events in apoptosis. Cell 134, 679-691; Simon, G. M., Dix, M. M., and Cravatt, B. F. (2009) Comparative assessment of large-scale proteomic studies of apoptotic proteolysis. ACS Chem. Biol. 4, 401-408) technology to globally profile proteolytic events occurring over the last 6-8 h of the intraerythrocytic cycle of P. falciparum. Using this method, we were able to generate peptographs for a large number of proteins at 6 h prior to rupture as well as at the point of rupture and in purified merozoites after exit from the host cell. These peptographs allowed assessment of proteolytic processing as well as changes in both protein localization and overall stage-specific expression of a large number of parasite proteins. Furthermore, by using a highly selective inhibitor of the cysteine protease dipeptidyl aminopeptidase 3 (DPAP3) that has been shown to be a key regulator of host cell rupture, we were able to identify specific substrates whose processing may be of particular importance to the process of host cell rupture. These results provide the first global map of the proteolytic processing events that take place as the human malarial parasite extracts itself from the host red blood cell. These data also provide insight into the biochemical events that take place during host cell rupture and are likely to be valuable for the study of proteases that could potentially be targeted for therapeutic gain.
Autoimmune Dilated Cardiomyopathy in PD-1 Receptor-Deficient Mice (2001)

Dilated cardiomyopathy is a severe pathology of the heart with poorly understood etiology. Disruption of the gene encoding the negative immunoregulatory receptor PD-1 in BALB/c mice, but not in BALB/c RAG-2-/- mice, caused dilated cardiomyopathy with severely impaired contraction and sudden death by congestive heart failure. Affected hearts showed diffuse deposition of immunoglobulin G (IgG) on the surface of cardiomyocytes. All of the affected PD-1-/- mice exhibited high-titer circulating IgG autoantibodies reactive to a 33-kilodalton protein expressed specifically on the surface of cardiomyocytes. These results indicate that PD-1 may be an important factor contributing to the prevention of autoimmune diseases.
A universal pretargeting system for cancer detection and therapy using bispecific antibody. (2003)

Multistep targeting systems represent highly selective alternatives to targeting systems using directly radiolabeled antibodies for diagnostic and therapeutic applications. A flexible bispecific antibody (bsMAb) multistep, pretargeting system that potentially can be developed for use with a variety of different imaging or therapeutic agents is described herein. The flexibility of this system is based on use of an antibody directed against histamine-succinyl-glycine (HSG) and the development of peptides containing the HSG residue. HSG-containing peptides were synthesized with either 1,4,7,10-tetraazacyclododecane-N,N',N",N"'-tetraacetic acid for the chelation of 111In, 90Y, or 177Lu, or a technetium/rhenium chelate. The peptides can be radiolabeled to a high specific activity in a facile manner that avoids the need for purification. In vivo studies in nude mice bearing human colon tumor xenografts showed that the radiolabeled peptides cleared rapidly from the body with minimal retention in tumor or normal tissues. For pretargeting, these peptides were used in combination with a bsMAb composed of the anti-HSG Fab' that was covalently coupled with the Fab' of either an anticarcinoembryonic antigen or an anticolon-specific antigen-p antibody to provide tumor targeting capability. When the radiolabeled peptides were administered 1-2 days after a pretargeting dose of the bsMAbs, tumor uptake of the radiolabeled peptides increased as much as 28-175-fold over that seen with the peptides alone with tumor:nontumor ratios exceeding 2:1 to 8:1 within just 3 h of the peptide injection, which was a marked improvement over the tumor:nontumor ratios seen with a directly radiolabeled 99mTc-anti-anticarcinoembryonic antigen Fab' at this same time. The anticolon-specific antigen-p x anti-HSG F(ab')2 bsMAb had the highest and longest retention in the tumor, and when used in combination with the 111In-labeled peptide, radiation dose estimates for therapeutic radionuclides, such as 90Y and 177Lu, suggested that antitumor effects would be expected with tolerable radiation exposure to the normal tissues. These results suggest that this multistep, pretargeting system has diagnostic imaging and therapeutic potential.
Somatomedin‐C and platelet‐derived growth factor stimulate human fibroblast replication (1981)

Previous studies have demonstrated that serum contains mitogens, such as platelet-derived growth factor (PDGF), which may alter fibroblast responsiveness to growth factors contained in plasma. Somatomedin-C (SM-C) has been identified as one of the plasma growth factors required for mouse Balb/c 3T3 fibroblasts to initiate DNA synthesis. The present experiments were undertaken to explore the interaction between PDGF, human growth hormone (hGH), SM-C, and other growth-promoting agents in stimulating the growth of human fibroblasts. Proliferation of human dermal fibroblasts plated at low density (3,000 cells/cm2) was found to be equally stimulated by continuous exposure to either normal or somatomedin-C-deficient serum. In contrast, when confluent monolayers were sequentially exposed to PDGF, followed either by normal platelet poor plasma (PPP) or hypopituitary PPP, the cells exposed to normal PPP entered the "S" phase of the cell cycle 50% faster. This difference could be abolished by a 6-hour incubation with growth hormone (10 ng/ml) or somatomedin-C (5 ng/ml) preceding the addition of plasma. When medium containing either hGH or Sm-C was changed frequently so as to remove factors secreted by fibroblasts only those cells exposed to exogeneous somatomedin-C entered DNA synthesis. This finding is in agreement with previous findings that human fibroblasts are capable of making Sm-C in response to hGH. These findings support the hypothesis that somatomedin is required for fibroblast replication in vitro, and that growth hormone appears to stimulate replication indirectly through somatomedin production.
Cytokine-induced expression of a nitric oxide synthase in rat renal tubule cells. (1993)

Nitric oxide (NO.) has been implicated in the regulation of renal vascular tone and tubular sodium transport. While the endothelial cell is a well known source of NO(.), recent studies suggest that tubular epithelial cells may constitutively generate NO(.). An inducible isoform of nitric oxide synthase which produces far greater quantities of NO. exists in some cell types. We sought to determine whether kidney epithelial cells exposed to cytokines could express an inducible nitric oxide synthase. Primary cultures of rat proximal tubule and inner medullary collecting duct cells generated NO. on exposure to TNF-alpha and IFN-gamma. NO. production by both cell types was inhibited by NG-monomethyl-L-arginine; this inhibition was partially reversed by the addition of excess L-arginine. Stimulation of kidney epithelial cells with TNF-alpha and IFN-gamma dramatically increased the level of inducible nitric oxide synthase mRNA. In summary, renal proximal tubule and inner medullary collecting duct cells can produce NO. via expression of an inducible isoform of nitric oxide synthase.
Substructure in the Radiation Survival Response at Low Dose: Asynchronous and Partially Synchronized V79-WNRE Cells (1993)

The radiation survival response of asynchronously-dividing populations of the cell line V79-WNRE indicates substructure in the low dose region that can be better characterized by separately fitting the data within the low and high dose regions to the linear-quadratic (LQ) equation. Flow cytometry and cell sorting techniques have been used to determine the response of both asynchronous populations and synchronous populations obtained by mitotic selection with or without an additional drug block. The statistically significant substructure which is present in the radiation response of asynchronous cells is absent in G1/S cells synchronized by mitotic selection followed by hydroxyurea (or aphidicolin) accumulation at the G1/S boundary. Such G1/S populations have a radiation response that is well characterized by a single LQ expression over 3 logs of cell inactivation. In contrast, the radiation response of cells synchronized by mitotic selection alone and irradiated in early G1 shows substantial substructure when fitted to the LQ equation, reflecting some radioresistance at high dose. While this response is well described by a single hit plus multitarget equation, it is possible that the small proportion (< 5%) of interphase cells in such mitotically-selected populations may be responsible for the resistance observed at higher doses.