gebhart/patrepeval huggingface dataset:
Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro (1980)

Small unilamellar neutral, negatively and positively charged liposomes composed of egg phosphatidylcholine, various amounts of cholesterol and, when appropriate, phosphatidic acid or stearylamine and containing 6-carboxyfluorescein were injected into mice, incubated with mouse whole blood, plasma or serum or stored at 4 degrees C. Liposomal stability, i.e. the extent to which 6-carboxyfluorescein is retained by liposomes, was dependent on their cholesterol content. (1) Cholesterol-rich (egg phosphatidylcholine/cholesterol, 7:7 molar ratio) liposomes, regardless of surface charge, remained stable in the blood of intravenously injected animals for up to at least 400min. In addition, stability of cholesterol-rich liposomes was largely maintained in vitro in the presence of whole blood, plasma or serum for at least 90min. (2) Cholesterol-poor (egg phosphatidylcholine/cholesterol, 7:2 molar ratio) or cholesterol-free (egg phosphatidylcholine) liposomes lost very rapidly (at most within 2min) much of their stability after intravenous injection or upon contact with whole blood, plasma or serum. Whole blood and to some extent plasma were less detrimental to stability than was serum. (3) After intraperitoneal injection, neutral cholesterol-rich liposomes survived in the peritoneal cavity to enter the blood circulation in their intact form. Liposomes injected intramuscularly also entered the circulation, although with somewhat diminished stability. (4) Stability of neutral and negatively charged cholesterol-rich liposomes stored at 4 degrees C was maintained for several days, and by 53 days it had declined only moderately. Stored liposomes retained their unilamellar structure and their ability to remain stable in the blood after intravenous injection. (5) Control of liposomal stability by adjusting their cholesterol content may help in the design of liposomes for effective use in biological systems in vivo and in vitro.
The molecular–genetic analysis of Triticum tauschii, the D-genome donor to hexaploid wheat (1991)

DNA from Triticum tauschii (the D-genome donor to hexaploid wheat, Triticum aestivum) has been cloned using the restriction endonuclease PstI to generate fragments for insertion into the plasmid pBR322 or pUC118. A total of 143 clones were studied and demonstrated to contain one of the following sequence types: (i) a D-genome amplified repetitive sequence, (ii) polymorphic repetitive sequences ("fingerprint-type" sequences), (iii) polymorphic low to moderately repetitive sequences (PLR sequences), (iv) polymorphic low copy number sequences (PL sequences), and (v) invariant sequences. The D-genome amplified sequence was found to be located on all seven chromosomes. A genetic linkage map using PL and PLR sequences was produced by analysing F 2 segregating progeny from crosses between two different taxa of T. tauschii. In addition to using DNA clones from T. tauschii, clones from other laboratories containing either anonymous sequences or genes coding for known products (e.g., 7S globulin, dehydrin, germin, storage protein) were used in the genetic linkage map. Multiple locations were mapped for the PLR sequences and were often clustered on single chromosomes. The restriction fragment length polymorphism markers and isozymes analysed were generally distributed over all the linkage groups that were identified and, when used in conjunction with published markers, provided the basis for a genetic map of T. tauschii.Key words: D genome, genetic linkage, restriction fragment length polymorphism, isozymes, chromosomal location.
Metastatic Behavior of Breast Cancer Subtypes (2010)

Breast cancer subtypes are associated with distinct patterns of metastatic spread with notable differences in survival after relapse.
Dendritic cell immunotherapy for stage IV melanoma (2007)

Active boosting of the antitumour immune response of patients with solid malignancies has been tested in a large number of trials. Isolated complete clinical responses have been reported, however, they have not been replicated in subsequent studies. We recently reported objective clinical responses to a dendritic cell/irradiated autologous tumour cell 'vaccine' in patients with distant metastatic (stage IV) melanoma. Here we describe our experience in a second cohort of patients with stage IV melanoma, using this dendritic cell-based immunotherapy in a cryopreserved format. Of 46 patients enrolled into the study, three had complete remission of all detectable disease, and a further three had partial clinical responses. These data confirm that dendritic cell-based immunotherapy has potential as a therapy in a limited number of patients with stage IV melanoma. To our knowledge, this is the first demonstration that cryopreserved dendritic cells can elicit complete clinical responses in patients with advanced cancer. Our observations support randomized controlled trials to validate the findings.
The spread of the Witty worm (2004)

On Friday, 19 March 2004, at approximately 8:45 p.m. Pacific Standard Time (PST), an Internet worm began to spread, targeting a buffer overflow vulnerability in several Internet Security Systems (ISS) products, including its RealSecure Network, RealSecure Server Sensor, RealSecure Desktop, and BlackICE. The worm took advantage of a security flaw in these firewall applications that eEye Digital Security discovered earlier in March. Once the Witty worm - so called because its payload contained the phrase, "(^,^)insert witty message here (^,^)" - infects a computer, it deletes a randomly chosen section of the hard drive, which, over time, renders the machine unusable. We share a global view of the worm's spread, with particular attention to its features.
An adaptive decision threshold control of the optical receiver for multi-gigabit terrestrial DWDM transmission systems (2002)

An adaptive decision threshold control scheme for multigigabit terrestrial optical amplifier transmission system is applied to a 2.5 Gb/s optical receiver. Significant BER performance improvement was achieved over 600 km transmission experiment. We have studied and developed a practical technique for maximize the, signal to noise ratio (SNR) at the decision circuit by an adaptive threshold control (ATC). Transmission experiment has been carried out over 600 km fibers, and significant BER improvement has been attained.
Development of a functional scoring system in dogs with acute spinal cord injuries (2001)

Pelvic limb gait of dogs recovering from thoracolumbar spinal cord injuries can be reliably quantified, using a numeric scale. This scale will facilitate the performance of clinical trials aimed at improving the outcome of acute spinal cord injuries.
Target-Cancer-Cell-Specific Activatable Fluorescence Imaging Probes: Rational Design and in Vivo Applications (2010)

Conventional imaging methods, such as angiography, computed tomography (CT), magnetic resonance imaging (MRI), and radionuclide imaging, rely on contrast agents (iodine, gadolinium, and radioisotopes, for example) that are "always on." Although these indicators have proven clinically useful, their sensitivity is lacking because of inadequate target-to-background signal ratio. A unique aspect of optical imaging is that fluorescence probes can be designed to be activatable, that is, only "turned on" under certain conditions. These probes are engineered to emit signal only after binding a target tissue; this design greatly increases sensitivity and specificity in the detection of disease. Current research focuses on two basic types of activatable fluorescence probes. The first developed were conventional enzymatically activatable probes. These fluorescent molecules exist in the quenched state until activated by enzymatic cleavage, which occurs mostly outside of the cells. However, more recently, researchers have begun designing target-cell-specific activatable probes. These fluorophores exist in the quenched state until activated within targeted cells by endolysosomal processing, which results when the probe binds specific receptors on the cell surface and is subsequently internalized. In this Account, we present a review of the rational design and in vivo applications of target-cell-specific activatable probes. In engineering these probes, researchers have asserted control over a variety of factors, including photochemistry, pharmacological profile, and biological properties. Their progress has recently allowed the rational design and synthesis of target-cell-specific activatable fluorescence imaging probes, which can be conjugated to a wide variety of targeting molecules. Several different photochemical mechanisms have been utilized, each of which offers a unique capability for probe design. These include self-quenching, homo- and hetero-fluorescence resonance energy transfer (FRET), H-dimer formation, and photon-induced electron transfer (PeT). In addition, the repertoire is further expanded by the option for reversibility or irreversibility of the signal emitted through these mechanisms. Given the wide range of photochemical mechanisms and properties, target-cell-specific activatable probes have considerable flexibility and can be adapted to specific diagnostic needs. A multitude of cell surface molecules, such as overexpressed growth factor receptors, are directly related to carcinogenesis and thus provide numerous targets highly specific for cancer. This discussion of the chemical, pharmacological, and biological basis of target-cell-specific activatable imaging probes, and methods for successfully designing them, underscores the systematic, rational basis for further developing in vivo cancer imaging.
Use of β‐lactamase as a secreted reporter of promoter function in yeast (1994)

K1 preprotoxin is the 316 residue precursor of the K1 killer toxin secreted by the yeast Saccharomyces cerevisiae. The SP beta la reporter consists of the mature, secreted form of beta-lactamase (beta la) fused to S and P, two fragments of preprotoxin. S is the N-terminal 34 residues, including the secretion signal. P, a 67 residue 'processing' segment with three sites for N-glycosylation, terminates in a Lys Arg site for cleavage by the Kex2 protease. Expression of SP beta 1a in yeast results in efficient secretion, processing by signal peptidase and glycosylation in the endoplasmic reticulum, producing pro beta la. Kex2 cleavage of pro beta la in the lumen of a late Golgi compartment releases beta la, which accumulates stably in culture media buffered at pH 5.8-7. The half-life of secretion is 11 min at 30 degrees C; 10-12% of the total activity in exponential-phase cells is intracellular, mostly in the form of pro beta la, indicating that transit from the endoplasmic reticulum to the Golgi is rate limiting. We have used SP beta la expression in single- and multi-copy vectors to compare the PGK, GAL1, GAL10, PHO5 and CUP1 promoters under varying nutritional conditions. In exponential-phase cells, secretion of beta la over a 40-fold range and up to several micrograms/ml was proportional to transcript level, demonstrating that SP beta la can be employed as a convenient secreted reporter of promoter function in yeast.
Solid freeforming of ceramics using a drop-on-demand jet printer (1997)

The formulation of a stabilized ceramic ink and the adaptation of a piezoelectric-activated drop- on-demand jet printer with a 65 mm nozzle for the direct solid freeforming of ceramics by multilayer jet printing is reported. The procedure has exciting implications for the miniaturization of ceramic circuits and for the fabrication of solid oxide fuel cells, ordered ceramic composites and small monolithic ceramic components of complex shape.
Fabrication and performance of 1.5μm GaInAsP travelling-wave laser amplifiers with angled facets (1987)

A broadband 1.5μm GaInAsP travelling-wave laser ampli-fier has been realised with angled facets instead of anti-reflection coatings. The measured signal gain for the TE mode is 19dB at the wavelength around 1.49μm with a 2dB gain ripple over one free spectral range and a 3dB band-width of 55 nm. The estimated residual modal reflectivity is 0.2%.
Induction of IgG Antibodies by an Anti-Idiotype Antibody Mimicking Disialoganglioside GD2 (1998)

The anti-idiotype (Id) monoclonal antibody (mAb) 1A7 immunoglobulin G1 (IgG1, kappa), raised in syngeneic mice against the murine anti-ganglioside GD2 mAb 14G2a mimics a carbohydrate epitope on GD2 and serves as a surrogate protein antigen for this disialoganglioside. Immunization of allogeneic C57BL/6 mice and rabbits with 1A7 induced anti-GD2 antibodies of IgG isotype that recognize purified GD2 by enzyme-linked immunosorbent assay (ELISA) and GD2-positive human melanoma cells (M21/P6) by fluorescence-activated cell sorter (FACS) analysis. The specificity of the antisera for GD2 was further confirmed by dot-blot analysis. These antisera also specifically lyse GD2-positive M21/P6 target cells in an antibody-dependent cellular cytotoxicity assay. Taken together, these results suggest that the anti-Id 1A7 can induce GD2-specific IgG antibodies that can recognize cell surface-associated as well as soluble disialoganglioside GD2.
Patricia G. Smith: Range Management editor extraordinaire. (1984)

This material was digitized as part of a cooperative project between the Society for Range Management, the National Agricultural Library, and the University of Arizona Libraries.
Preparation of Apatite Thin Films through rf‐Sputtering from Calcium Phosphate Glasses (1994)

Apatite thin films of calcium phosphate were successfully prepared by rf‐magnetron sputtering, followed by postannealing, from calcium phosphate glass targets with Ca/P atomic ratios of 0.6 to 0.75, much lower than the stoichio‐metric value of 1.67 for hydroxyapatite. Using targets with low Ca/P ratios of 0.65 to 0.25, tricalcium and pyrocalcium phosphate thin films were obtained. The formation of these calcium phosphates phases was attributed to the phosphorus oxide losses during sputtering. H 2 O vapor was effective in the formation of the apatite phase at high temperatures; apatite was the exclusive phase in the ambient H 2 O, even when the target with the low Ca/P ratio of 0.4 was used. Based on phase stability at high temperatures, it was assumed that various kinds of apatites, such as stoichiometric and nonstoichiometric hydroxy‐ and Ca‐deficient hydroxy‐apatites were formed, depending on the composition of the glass targets.
Radiopharmaceuticals to monitor gene transfer. (1997)

Advances in genetic engineering and molecular biology have opened the door to disease treatment by transferring genes to cells that are responsible for the pathological condition being addressed. These genes can serve to supplement or introduce the function of indigenous genes that are either inadequately expressed or that are congenitally absent in the patient. Additionally, however, they can introduce new functions such as drug sensitization, to provide a unique therapeutic target. Gene transfer is readily monitored in vitro using a range of histochemical and biochemical tests that are 'built in' to the therapeutic gene cassette. In vivo, in situ monitoring of the gene transfer and gene expression processes can be achieved with these tests only if biopsy is possible. Scintigraphic imaging can offer unique information on both the extent and location of gene expression, provided that an appropriate reporter gene is included in the therapeutic cassette. This overview includes a brief orientation to gene transfer therapy and is followed by a review of current approaches to gene therapy imaging. The concluding section deals with imaging based on radiolabelled nucleoside substrates for herpes simplex type-1 thymidine kinase, with emphasis on IVFRU, a stable, potent and selective HSV-1 TK substrate developed in our laboratories.
Control of programmed cell death by the baculovirus genes p35 and iap. (1994)

The SF-21 insect cell line undergoes rapid and widespread apoptosis when treated with actinomycin D or when infected with a mutant of the baculovirus Autographa californica nuclear polyhedrosis virus lacking a p35 gene or a functionally active iap (inhibitor of apoptosis) gene. Here we provide evidence that the basis for the induction of apoptosis by these two different stimuli is the cessation of RNA synthesis. We also show that expression of either p35 or two different functional iap homologs blocks apoptosis independently of other viral genes, indicating that these gene products act directly on the cellular apoptotic pathway. The iap genes encode a C3HC4 (or RING) finger motif found in a number of transcriptional regulatory proteins, as well as two additional Cys/His motifs (baculovirus iap repeats). We show that specific amino acids within both the C3HC4 finger and the N-terminal baculovirus iap repeat are critical for anti-apoptosis function. Overexpression of either mammalian bcl-2 or adenovirus E1B-19K, genes which block apoptosis when overexpressed in a number of mammalian cells, does not block actinomycin D-induced apoptosis in SF-21 cells.
Advances in Therapy for Psoriasis: An Overview of Infliximab, Etanercept, Efalizumab, Alefacept, Adalimumab, Tazarotene, and Pimecrolimus (2005)

Psoriasis is a chronic skin disorder that affects approximately 2% of the US and European population. Over the last several years, one of the major focuses in psoriasis research has been the development of biologic therapies for this disease. The aim of these therapies is to provide selective, immunologically directed intervention with fewer side effects than traditional therapies. The goal of this article is to review the progress of the biologic agents which are available, or under investigation for clinical use: infliximab, etanercept, efalizumab, alefacept, and adalimumab. In addition, two other investigational therapies, oral tazarotene and oral pimecrolimus will be discussed. Clinical data for these agents, including the most recent phase II and/or III study results, will be discussed, as well as the most recent safety data.
Utilization of 1, N6-Etheno-2'-deoxyadenosine 5'-triphosphate during DNA synthesis on natural templates, catalyzed by DNA polymerase I of Escherichia coli (1986)

To test whether vinyl chloride-induced mutagenesis might involve ambiguous base pairing of 1,N6-etheno-adenine (epsilon A) during DNA synthesis, we examined the base pairing potential of epsilon dATP during DNA synthesis catalyzed by Escherichia coli DNA polymerase I (Klenow fragment). An electrophoretic assay of chain elongation was used to assess the degree to which epsilon dATP could substitute for each of the normal dNTPs during elongation of a primer annealed to a bacteriophage template. Despite the fact that the etheno bridge completely blocks normal Watson-Crick pairing of epsilon A with T, we observed that epsilon dATP could substitute for dATP during primer elongation (although inefficiently). In addition, detectable substitution of epsilon dATP for dGTP and dCTP occurred, indicating that epsilon A exhibits ambiguous base pairing properties. The relative ease of epsilon dAMP incorporation (opposite template T, C and G) appeared to vary considerably at different positions along the template. The major form of epsilon A incorporation (replacement of A) was confirmed by measurements of epsilon dATP----epsilon dAMP turnover (a commonly used method for detecting misincorporation), and also by the demonstration that epsilon A was present in enzymatic hydrolysates prepared from DNA that was synthesized with epsilon dATP replacing dATP. A model for ambiguous base pairing of epsilon dATP is proposed, in which incorporation occurs via the protonated, syn form of epsilon dATP.
Amorphous Inorganic Materials and Glasses (1993)

Methods of preparing amorphous solids and glasses thermodynamic description of the glassy state phase transformation processes and glass formation structure and chemical bonding in glasses and amorphous solids eletronic states in noncrystalline solids amorphous and glass-forming substances electrical conductivity and optical properties of glasses and amorphous solids applications of ion-conducting glasses optical glasses for IR transmission information storage based on structural changes of glasses.
Expression of Aurora kinases in human thyroid carcinoma cell lines and tissues (2006)

The Aurora kinases are involved in the regulation of cell cycle progression, and alterations in their expression have been shown to associate with cell malignant transformation. In the present study, we demonstrated that human thyrocytes express all 3 Aurora kinases (A, B and C) at both protein and mRNA level and this expression is cell cycle-regulated. An increase in the protein level of the 3 kinases was found, with respect to normal human thyrocytes (HTU5), in the human cell lines derived from follicular (FTC-133), papillary (B-CPAP) and anaplastic (8305C) thyroid carcinomas, but not in cells derived from a follicular adenoma (HTU42). These observations were mirrored in RT-PCR experiments for Aurora-A and B. In contrast, Aurora-C mRNA levels were not significantly different among the different cell types analyzed, suggesting that posttranscriptional mechanism(s) modulate its expression. The expression at the protein level of all 3 Aurora kinases was significantly higher in 3 thyroid papillary carcinomas with respect to normal matched tissues obtained from the same patients. Similar modifications, at the mRNA level, could be observed in 7 papillary carcinoma tissues for Aurora-A and B, but not for Aurora-C. In conclusion, we demonstrated that normal human thyrocytes express all 3 members of the Aurora kinase family, and their expression is amplified in malignant thyroid cell lines and tissues. These results suggest that the Aurora kinases may play a relevant role in malignant thyroid cancers, and may represent a putative therapeutic target for thyroid neoplasms.
Micromachined thin film bulk acoustic resonators (2002)

We have fabricated thin film bulk acoustic resonators (FBARS) with measured Q's of over 1000 and resonant frequencies as low as 1.5 GHz and as high as 7.5 GHz. The device, as currently fabricated, consists of the piezoelectric material aluminum nitride (AlN) sandwiched between electrodes all of which lie on a thin low-stress silicon nitride (Si/sub x/N/sub y/) membrane. Integrated on the membrane are small microheaters for frequency tuning and/or temperature stabilization. We have observed frequency shifts of 50 to 80 ppm per degree C depending on relative material thicknesses. Maximum temperature excursions over 580 C could be achieved using the microheaters. We have also observed frequency shifts of 5 to 10 ppm per volt depending on harmonic.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>