gebhart/patrepeval huggingface dataset:
Activin receptor-like kinase 1 modulates transforming growth factor-β1 signaling in the regulation of angiogenesis (2000)

The activin receptor-like kinase 1 (ALK1) is a type I receptor for transforming growth factor-beta (TGF-beta) family proteins. Expression of ALK1 in blood vessels and mutations of the ALK1 gene in human type II hereditary hemorrhagic telangiectasia patients suggest that ALK1 may have an important role during vascular development. To define the function of ALK1 during development, we inactivated the ALK1 gene in mice by gene targeting. The ALK1 homozygous embryos die at midgestation, exhibiting severe vascular abnormalities characterized by excessive fusion of capillary plexes into cavernous vessels and hyperdilation of large vessels. These vascular defects are associated with enhanced expression of angiogenic factors and proteases and are characterized by deficient differentiation and recruitment of vascular smooth muscle cells. The blood vessel defects in ALK1-deficient mice are reminiscent of mice lacking TGF-beta1, TGF-beta type II receptor (TbetaR-II), or endoglin, suggesting that ALK1 may mediate TGF-beta1 signal in endothelial cells. Consistent with this hypothesis, we demonstrate that ALK1 in endothelial cells binds to TGF-beta1 and TbetaR-II. Furthermore, the ALK1 signaling pathway can inhibit TGF-beta1-dependent transcriptional activation mediated by the known TGF-beta1 type I receptor, ALK5. Taken together, our results suggest that the balance between the ALK1 and ALK5 signaling pathways in endothelial cells plays a crucial role in determining vascular endothelial properties during angiogenesis.
InGaAs/GaAs Quantum Dot Lasers with Ultrahigh Characteristic Temperature (T 0= 385 K) Grown by Metal Organic Chemical Vapour Deposition (1997)

Low threshold current density (AlInGa)As/GaAs lasers based on InGaAs quantum dots (QDs) are grown by metal organic chemical vapour deposition (MOCVD). Quantum dots deposited at 490° C and covered with GaAs are directly revealed in the active region. On a transmission electron microscopy (TEM) image of the laser structure no large clusters or dislocations are found over a macroscopic distance. We show that the properties of QD lasers can be strongly improved if the QDs are confined by Al 0.3 Ga 0.7 As barriers and the cladding layers are grown at high temperature. Optimisation of the laser structure geometry allows extension of the range of ultrahigh temperature stability ( T 0 =385 K) of the threshold current to 50° C.
Finding "Who Is Talking to Whom" in VoIP Networks via Progressive Stream Clustering (2006)

Technologies that use the Internet network to deliver voice communications have the potential to reduce costs and improve access to communications services around the world. However, these new technologies pose several challenges in terms of confidentiality of the conversations and anonymity of the conversing parties. Call authentication and encryption techniques provide a way to protect confidentiality, while anonymity is typically preserved by an anonymizing service (anonymous call). This work studies the feasibility of revealing pairs of anonymous and encrypted conversing parties (caller/callee pair of streams) by exploiting the vulnerabilities inherent to VoIP systems. In particular, by exploiting the aperiodic inter-departure time of VoIP packets, we can trivialize each VoIP stream into a binary time-series. We first define a simple yet intuitive metric to gauge the correlation between two VoIP binary streams. Then we propose an effective technique that progressively pairs conversing parties with high accuracy and in a limited amount of time. Our metric and method are justified analytically and validated by experiments on a very large standard corpus of conversational speech. We obtain impressively high pairing accuracy that reaches 97% after 5 minutes of voice conversations.
A Fully-Integrated, Miniaturized (0.125 mm²) 10.5 µW Wireless Neural Sensor (2013)

A wirelessly powered 0.125 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> 65 nm CMOS IC for Brain-Machine Interface applications integrates four 1.5 μW amplifiers (6.5 μVrms input-referred noise with 10 kHz bandwidth) with power conditioning and communication circuitry. The multi-node backscatter frequency locks to a wireless interrogator using a frequency-domain multiple access communication scheme. The full system, verified with wirelessly powered in vivo recordings, consumes 10.5 μW and operates at 1 mm range in air with 50 mW transmit power.
In welcher Reihenfolge fragen? Kontexteffekte in standardisierten Befragungen (1991)

Der vorliegende Beitrag berichtet über neuere kognitionspsychologische Untersuchungen zur Frage, unter welchen Bedingungen Kontexteffekte bei standardisierten Befragungen auftreten. Dabei wird auch versucht, die Richtung dieser Einflüsse vorherzusagen. Der Autor konzentriert sich speziell auf den Abruf von Informationen aus dem Gedächtnis und die Nutzung dieser Informationen in der Urteilsbildung. Es zeigt sich, daß Antworten je nach Fragereihenfolge unterschiedlich ausfallen. Dies spiegelt wider, daß menschliches Denken stets in einem Kontext erfolgt und wir unser Leben oder unsere Politiker anders sehen, je nachdem, an welche Facette wir gerade denken. Unterschiedliche Reihenfolgen fangen deshalb unterschiedliche Facetten der Realität ein. Abschließend wird gefordert, Kontexteffekte bei Befragungen gezielt zu nutzen. Sie sind ein hervorragendes methodisches Instrument, das zur Prüfung vermuteter Zusammenhänge eingesetzt werden kann. (psz)
A reduced latency list decoding algorithm for polar codes (2014)

The cyclic redundancy check (CRC) aided successive cancelation list (SCL) decoding algorithm has better error performance than the successive cancelation (SC) decoding algorithm for short or moderate polar codes. However, the CRC aided SCL (CA-SCL) decoding algorithm still suffer from long decoding latency. In this paper, a reduced latency list decoding (RLLD) algorithm for polar codes is proposed. For the proposed RLLD algorithm, all rate-0 nodes and part of rate-1 nodes are decoded instantly without traversing the corresponding subtree. A list maximum-likelihood decoding (LMLD) algorithm is proposed to decode the maximum likelihood (ML) nodes and the remaining rate-1 nodes. Moreover, a simplified LMLD (SLMLD) algorithm is also proposed to reduce the computational complexity of the LMLD algorithm. Suppose a partial parallel list decoder architecture with list size L = 4 is used, for an (8192, 4096) polar code, the proposed RLLD algorithm can reduce the number of decoding clock cycles and decoding latency by 6.97 and 6.77 times, respectively.
A mutation in guanylate cyclase activator 1A (GUCA1A) in an autosomal dominant cone dystrophy pedigree mapping to a new locus on chromosome 6p21.1 (1998)

We report a mutation (Y99C) in guanylate cyclase activator 1A (GUCA1A), the gene for guanylate cyclase activating protein (GCAP1), in a family with autosomal dominant cone dystrophy. Linkage analysis excluded all the known cone and cone-rod dystrophy loci, except the chromosome 6p21.1 region. This is known to contain the RDS gene, which is associated with dominant cone-rod dystrophy. Screening of the RDS gene by heteroduplex analysis and direct sequencing failed to demonstrate sequence changes in the coding region of this gene. The gene for GCAP1, a calcium binding protein which is highly expressed in photoreceptor outer segments, is also located in 6p21.1. It was screened for mutations, and all affected individuals showed a single base pair missense mutation (A-->G) at codon 99 in exon 2 of this gene generating a tyrosine-to-cysteine change in the GCAP1 protein. This change was absent from 206 unrelated normal controls. We propose that this change would at least disrupt the EF3handof GCAP1 thereby preventing calcium binding and consequently interfere with activation. The resulting effect on cGMP production would predictably modify the number of open cGMP gated cation channels, and could explain the ultimate demise of cone photoreceptor cells.
New Approaches to Pathogenesis and Management of Hypertension (2009)

# Catheter-based renal sympathetic denervation for resistant hypertension: A multicenter safety and proof-of-principle cohort study. Lancet 373: 1275–1281, 2009 {#article-title-2} 1886 1888 

Upon the initiative of Smithwick and Thompson (1) of the Massachusetts General Hospital, resection of the splanchnic nerves through a posterior infradiaphragmatic approach plus removal of the sympathetic chain from the level of the eighth dorsal ganglion to the second lumbar ganglion had been used with relative frequency in cases of desperate hypertension at the time when antihypertensive medication was not yet available. In the hands of other investigators, the results were spectacular in a minority of patients but not quite satisfactory in many patients (2,3). Despite improvement of headache, reversal of papilledema in malignant hypertension, etc. , the long-term reduction of BP was quite variable and the 5-yr mortality remained approximately 40% (2). A 10-yr follow-up compared 100 patients who were subjected to thoracolumbar sympathectomy with 1500 patients who received symptomatic therapy. Lasting BP reduction was seen only in one third of the patients (4). Whereas the average BP levels were reduced, occasional BP spikes were not. The average difference of preoperative to postoperative systolic BP values was 21 mmHg. The authors saw reduction of cerebrovascular accidents and less progression of proteinuria and renal dysfunction, but 10-yr mortality was still 41%. Against this background, once effective antihypertensive medication was available, this relatively crude procedure fell out of favor and remained a sleeping beauty.

With today's better insight into the role of sympathetic activity in the genesis of hypertension and particularly the role of the kidney in sympathetic activation, there has been a renaissance in the interest of the renal sympathetic nervous system, including its role in primary hypertension—apart from its undoubted role in the hypertension of chronic kidney disease (5–10). Renal disease and, in animal experiments, even minor renal tissue damage such as injection of minute volumes of phenol, trigger afferent signals that ascend via …
Strand displacement amplification as an in vitro model for rolling-circle replication: deletion formation and evolution during serial transfer. (1994)

Strand displacement amplification is an isothermal DNA amplification reaction based on a restriction endonuclease nicking its recognition site and a polymerase extending the nick at its 3' end, displacing the downstream strand. The reaction resembles rolling-circle replication of single-stranded phages and small plasmids. The displaced sense strand serves as target for an antisense reaction and vice versa, resulting in exponential growth and the autocatalytic nature of this in vitro reaction as long as the template is the limiting agent. We describe the optimization of strand displacement amplification for in vitro evolution experiments under serial transfer conditions. The reaction was followed and controlled by use of the fluorescent dye thiazole orange binding to the amplified DNA. We were able to maintain exponential growth conditions with a doubling time of 3.0 min throughout 100 transfers or approximately 350 molecular generations by using an automatic handling device. Homology of in vitro amplification with rolling-circle replication was mirrored by the occurring evolutionary processes. Deletion events most likely caused by a slipped mispairing mechanism as postulated for in vivo replication took place. Under our conditions, the mutation rate was high and a molecular quasi-species formed with a mutant lacking internal hairpin formation ability and thus outgrowing all other species under dGTP/dCTP deficiency.
Immunotherapeutic Strategies for Prevention and Treatment of Alzheimer's Disease (2001)

Pathologic examination in Alzheimer's disease (AD) shows a significant correlation between beta-amyloid peptide (AbetaP) deposition and the clinical severity of dementia. Formation of beta-amyloid (Abeta) is a complex kinetic and thermodynamic process, dependent on peptide-peptide interactions that may be modulated by other proteins. We found that site-directed antibodies toward peptide EFRH sequences 3-6 of the N-terminal region of AbetaP suppress in vitro formation of Abeta and dissolve already-formed fibrillar amyloid. These so-called chaperone-like properties of monoclonal antibodies led to the development of a new immunologic approach to AD treatment. The immunization procedure, based on phages displaying the EFRH epitope as antigen, induced anti-AbetaP antibodies that recognized the whole AbetaP and exhibited antiaggregating properties similar to those of antibodies obtained by injection of Abeta fibrils. Production and performance of anti-beta-amyloid antibodies in the transgenic mouse model of AD showed that these antibodies may be delivered from the periphery to the central nervous system, preventing the formation of Abeta and dissolving already-present aggregates. Moreover, immunization with Abeta protected transgenic mice from the learning and age-related memory deficits that occur in AD. These data support the hypotheses that Abeta plays a central role in AD and that site-directed antibodies that modulate Abeta conformation may provide immunotherapy of the disease.
Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation (2018)

Genetically modified pigs have been considered favorable resources in xenotransplantation. Microinjection of randomly integrating transgenes into zygotes, somatic cell nuclear transfer, homologous recombination, zinc finger nucleases, transcription activator-like effector nucleases, and most recently, clustered regularly interspaced short palindromic repeats-cas9 (CRISPR/Cas9) are the techniques that have been used to generate these animals. Here, we provide an overview of the CRISPR approaches that have been used to modify genes which are vital in improving xenograft survival rate, including cytidine monophosphate-<i>N</i>-acetylneuraminic acid hydroxylase, B1,4N-acetylgalactosaminyltransferase, isoglobotrihexosylceramide synthase, class I MHC, von Willebrand factor, C3, and porcine endogenous retroviruses. In addition, we will mention the importance of potential candidate genes which could be targeted using CRISPR/Cas9.
A continuum body force sensor designed for flexible surgical robotics devices (2014)

This paper presents a novel three-axis force sensor based on optical photo interrupters and integrated with the robot arm STIFF-FLOP (STIFFness controllable Flexible and Learnable Manipulator for Surgical Operations) to measure external interacting forces and torques. The ring-shape bio-compatible sensor presented here embeds the distributed actuation and sensing system of the STIFF-FLOP manipulator and is applicable to the geometry of its structure as well to the structure of any other similar soft robotic manipulator. Design and calibration procedures of the device are introduced: experimental results allow defining a stiffness sensor matrix for real-time estimation of force and torque components and confirm the usefulness of the proposed optical sensing approach.
Covid-19 and social mobility (2020)

Governments are having to balance short- and long-term interests, and the trade-offs they generate, as they try to recover from the Covid-19 pandemic. Social distancing measures may limit the number of immediate deaths from the coronavirus, but an extended lockdown could cause lasting damage to livelihoods for years to come. The effects vary across age groups: while the coronavirus health shock has particularly affected the over-60s, the longer-term economic and social damage is likely to hit young people disproportionately, especially the under-25s.
Selective DNA Release from DQAsome/DNA Complexes at Mitochondria-Like Membranes (2000)

The number of diseases found to be associated with defects of the mitochondrial genome has grown significantly over the past decade (Wallace 1999). Despite major advances in understanding mtDNA defects at the genetic and biochemical level, there is no satisfactory treatment available for the vast majority of patients and the exploration of gene therapeutic approaches is highly warranted. However, mitochondrial gene therapy still appears only theoretical and speculative. Any possibility for gene replacement depends on the use of a yet unavailable mitochondria-specific transfection vector. Mitochondria-specific vectors must posses two properties: they have to transport DNA to the side of mitochondria; they must not release DNA during endocytosis. Amphiphile compounds with delocalized cationic charge centers such as rhodamine 123 and the bolaamphiphile dequalinium have long been known to accumulate in mitochondria. Sufficient lipophilicity combined with delocalization of the positive charge to reduce the free energy change when moving from an aqueous to a hydrophobic environment are believed to be prerequisite for mitochondrial accumulation in response to the mitochondrial membrane potential. We have recently succeeded in preparing cationic vesicles made of dequalinium that we termed DQAsomes (Weissig et al. 1998a). We have shown that DQAsomes bind and protect DNA against DNase activity (Lasch et al. 1999). Based on the intrinsic property of dequalinium to preferentially accumulate in mitochondria in response to the electrochemical gradient at the mitochondrial membrane, we believe that DQAsomes can serve as a vector to deliver DNA to mitochondria in living cells. As a first step in the development of mitochondria-specific DNA delivery systems, we report here that DQAsome/DNA complexes selectively release DNA at cardiolipin-rich liposomes mimicking both the inner and the outer mitochondrial membrane. We demonstrate that DNA remains tightly associated with DQAsomes in the presence of an excess of anionic lipids other than cardiolipin.
Monoclonal Antibodies to the Rabbit Liver Growth Hormone Receptor: Production and Characterization* (1984)

Monoclonal antibodies to the GH receptor (GHR) have been produced by the application of hybridoma technology to splenic lymphocytes from BALB/C mice immunized with a human (hGH) affinity purified preparation of rabbit liver GHR. Primary screening of 384 wells yielded 4 antibodies able to immunoprecipitate [125I]iodo-hGH complexes with purified GHR and one able to inhibit binding of [125I]iodoovine GH ([125I]iodo-oGH) to rabbit liver microsomes. These cells were cloned and grown as ascitic tumors with loss of 1 of the 4 precipitators. Ascitic fluids contained monoclonal antibodies of high titer (inhibitor and 2 precipitators, 1:2.0 X 10(5); one precipitator, 1:2.0 X 10(4)) and high affinity (precipitators, 2.5-6.0 X 10(9) M-1; inhibitor, high affinity component, 6.4 X 10(10) M-1), which were isotyped as IgG1K and IgG2aK (1 precipitator). These antibodies did not cross-react with rabbit insulin or PRL receptors in the appropriate receptor assays and did not possess antihormone activity. Binding of [125I]iodo-MAb7, the inhibitory antibody, was totally blocked by the addition of excess unlabeled oGH or hGH, although these hormones had no effect on binding of the 125I-labeled precipitators. Scatchard analysis of [125I]iodo-oGH binding in the presence of MAb7 showed decreased binding by loss of sites rather than affinity. Antibody dilution curves and Scatchard plots for MAb7 binding provided evidence for two types of GHR in the rabbit liver, in accord with previously published data based on hormone binding studies. All precipitating antibodies gave an enhancement of [125I] iodo-oGH binding with purified receptor (up to 360% of polyethylene glycol-precipitated control), but only minimal enhancement with solubilized microsomal membranes. This enhancement was shown to be due to an increase in receptor number rather than affinity. After examining a number of hypotheses, we concluded that the enhancement was an artifact resulting from a nonpolyethylene glycol-precipitable species of GHR which could be totally precipitated by the monoclonal antibodies. We have produced and characterized four monoclonal antibodies to the GHR which will be of value in characterizing the structure and function of this receptor.
Effects of Deregulation of Methionine Biosynthesis on Methionine Excretion in Escherichia coli (2005)

Several regulators of methionine biosynthesis have been reported in Escherichia coli, which might represent barriers to the production of excess l-methionine (Met). In order to examine the effects of these factors on Met biosynthesis and metabolism, deletion mutations of the methionine repressor (metJ) and threonine biosynthetic (thrBC) genes were introduced into the W3110 wild-type strain of E. coli. Mutations of the metK gene encoding S-adenosylmethionine synthetase, which is involved in Met metabolism, were detected in 12 norleucine-resistant mutants. Three of the mutations in the metK structural gene were then introduced into metJ and thrBC double-mutant strains; one of the resultant strains was found to accumulate 0.13 g/liter Met. Mutations of the metA gene encoding homoserine succinyltransferase were detected in alpha-methylmethionine-resistant mutants, and these mutations were found to encode feedback-resistant enzymes in a 14C-labeled homoserine assay. Three metA mutations were introduced, using expression plasmids, into an E. coli strain that was shown to accumulate 0.24 g/liter Met. Combining mutations that affect the deregulation of Met biosynthesis and metabolism is therefore an effective approach for the production of Met-excreting strains.
The p47 co‐factor regulates the ATPase activity of the membrane fusion protein, p97 (1998)

The highly conserved ATPase p97, a member of the AAA-ATPases, is found in a complex with its co-factor p47 in rat liver cytosol. Previously it had been shown that p97-mediated reassembly of Golgi cisternae from mitotic Golgi fragments requires p47 which mediates the binding of p97 to a Golgi t-SNARE (soluble N-ethylmaleimide-sensitive factor attachment factor receptor), syntaxin 5. Here we show that it also suppresses the ATPase activity of p97 by up to 85% in a dose-dependent and saturable manner suggesting that it has other roles in the membrane fusion cycle.
Vibrotactile transduction and transducers (2007)

The body's sense of touch is potentially a versatile channel for the conveyance of directional, spatial, command, and timing information. Most practical implementations of vibrotactile systems require compact, light-weight actuators that can be mounted against the body. Eccentric mass motors are widely used for this application, yet their output is limited and the effects of loading on the transducers due to the skin and mounting arrangement have been largely ignored. Conventional linear actuators are well suited as vibrotactile transducers and can provide high output, but are typically limited to laboratory research due to their large size and cost. The effect of loading on various practical vibrotactile transducers is investigated using a skin impedance phantom and measuring the transducer displacement with respect to additional mass loading. Depending on the transducer design, loading can dramatically reduce the vibratory displacement and, in the case of eccentric mass motors, also increase the operating frequency. In contrast, a new linear actuator design can be designed to be almost independent of skin loading, by considering the mechanical impedance of the load and optimizing the transducer contact area.