gebhart/patrepeval huggingface dataset:
Relationships between intermediate TCR cells and NK1.1+ T cells in various immune organs. NK1.1+ T cells are present within a population of intermediate TCR cells (1995)

Experiments to date have revealed a population of T cells that carry intermediate (int) levels of TCR (or CD3) and express IL-2R beta-chain (IL-2R beta) in mouse liver. Such int TCR cells also reside in other immune organs, although in low numbers. On the other hand, NK1.1+ T cells with int TCR do reside in the thymus and other peripheral organs. To determine the relationship of two types of cells, we characterized int CD3 cells and NK1.1+ T cells throughout the organs in terms of the phenotype, V beta repertoire, and morphology. Although both IL-2R beta+ T cells and NK1.1+ T cells are classified as int CD3 cells, NK1.1+ T cells are present within int CD3 cells. The majority of int CD3 cells in the liver and thymus were NK1.1+, whereas the minority of such cells in the spleen, lymph nodes, and bone marrow were NK1.1+. Among int CD3 cells, double-negative (DN) CD4-8- cells and/or CD4+ were abundant in NK1.1+ subset, whereas CD8+ cells were generally abundant in NK1.1- subset. Self-reactive V beta+ clones estimated by the M1s system were distributed to both NK1.1+ and NK1.1- subsets. High CD3 cells in the thymus and other organs contained neither DN cells nor forbidden clones. Int CD3 cells had the morphology of granular or agranular lymphocytes carrying perforin. Among int CD3 cells, NK1.1+ subset had a higher level of perforin-positive cells than NK1.1- subset. These results clearly demonstrate the relationship between int TCR cells and NK1.1+ T cells in various organs.
Discovery of 6-(4-{[5-Cyclopropyl-3-(2,6-dichlorophenyl)isoxazol-4-yl]methoxy}piperidin-1-yl)-1-methyl-1H-indole-3-carboxylic Acid: A Novel FXR Agonist for the Treatment of Dyslipidemia (2015)

The farnesoid X receptor (FXR) is a member of the "metabolic" subfamily of nuclear receptors. Several FXR agonists have been reported in the literature to have profound effects on plasma lipids in animal models. To discover novel and effective therapies for dyslipidemia and atherosclerosis, we have developed a series of potent FXR agonists that robustly lower plasma LDL and vLDL in LDLr-/- mice. To this end the novel piperidinylisoxazole system LY2562175 was discovered. This molecule is a potent and selective FXR agonist in vitro and has robust lipid modulating properties, lowering LDL and triglycerides while raising HDL in preclinical species. The preclinical ADME properties of LY2562175 were consistent with enabling once daily dosing in humans, and it was ultimately advanced to the clinic for evaluation in humans. The synthesis and biological profile of this molecule is discussed.
Analysis of OFDM in the presence of frequency offset and a method to reduce performance degradation (2002)

An orthogonal frequency division multiplexing (OFDM) system is sensitive to frequency errors, causing intercarrier interference (ICI) among subcarriers. We analyze the performance degradation of an OFDM system due to frequency offset errors and propose a new ICI cancellation method to reduce the effect of frequency offset errors. The main idea is to map each data symbol a/sub k/ which is to be transmitted onto a pair of non-adjacent subcarriers k and (N-1-k), with weightings +1 and -1, other than to a single subcarrier. The carrier-to-interference ratio (CIR) of the proposed method is better than that of normal OFDM by an amount between 10 dB to 30 dB. This method has another advantage of the frequency diversity effect in the multipath fading channel. This study shows that the proposed method performs better than the previously described self-ICI cancellation method for a small value of frequency offset.
Overview of radiolocation in CDMA cellular systems (1998)

Applications for the location of subscribers of wireless services continue to expand. Consequently, location techniques for wireless technologies are being investigated. With code-division multiple access (CDMA) being deployed by a variety of cellular and PCS providers, developing an approach for location in CDMA networks is imperative. This article discusses the applications of location technology, the methods available for its implementation in CDMA networks, and the problems that are encountered when using CDMA networks for positioning.
HER‐2/ neu Signal Transduction in Human Breast and Ovarian Cancer (1997)

The HER-2/neu proto-oncogene encodes a 185 kDa transmembrane receptor tyrosine kinase with significant sequence homology to other members of the class I receptor tyrosine kinase family. The HER-2/neu gene is amplified and/or overexpressed in 25%-30% of human breast and ovarian cancers, and overexpression of the receptor is associated with poor prognosis. Tyrosine phosphorylation and activation of the HER-2 receptor lead to activation of specific signal transduction pathways in breast and ovarian cancer cells, including the ras/MAP kinase cascade, phosphatidylinositol 3-kinase, and phospholipase C-gamma. HER-2/neu signal transduction pathways ultimately converge on the cell nucleus, where the expression of diverse genes is induced after activation of the receptor. A more complete understanding of HER-2/neu signal transduction pathways may allow the development of specific therapeutics for the treatment of those human breast and ovarian cancers containing this alteration.
Enumeration of Salmonella Bacteria in Food and Feed Samples by Real-Time PCR for Quantitative Microbial Risk Assessment (2007)

Quantitative microbial risk assessment (QMRA) is an important approach for food safety where the risk and factors that influence it are identified...
Estrogen Receptor α Promotes Breast Cancer by Reprogramming Choline Metabolism (2016)

Estrogen receptor α (ERα) is a key regulator of breast growth and breast cancer development. Here, we report how ERα impacts these processes by reprogramming metabolism in malignant breast cells. We employed an integrated approach, combining genome-wide mapping of chromatin-bound ERα with estrogen-induced transcript and metabolic profiling, to demonstrate that ERα reprograms metabolism upon estrogen stimulation, including changes in aerobic glycolysis, nucleotide and amino acid synthesis, and choline (Cho) metabolism. Cho phosphotransferase CHPT1, identified as a direct ERα-regulated gene, was required for estrogen-induced effects on Cho metabolism, including increased phosphatidylcholine synthesis. CHPT1 silencing inhibited anchorage-independent growth and cell proliferation, also suppressing early-stage metastasis of tamoxifen-resistant breast cancer cells in a zebrafish xenograft model. Our results showed that ERα promotes metabolic alterations in breast cancer cells mediated by its target CHPT1, which this study implicates as a candidate therapeutic target. Cancer Res; 76(19); 5634-46. ©2016 AACR.
Physiology of Thrombospondin (1990)

Thrombospondin is a large, multifunctional glycoprotein released from activated platelets and secreted by growing cells. It binds to components of the cell surface and extracellular milieu. Thrombospondin probably modulates a number of processes, including aggregation of platelets, formation and lysis of fibrin, adhesion and migration of cells, and progression of cells through the growth cycle. Studies relating thrombospondin to disease processes are just beginning.
Trait interindividual differences in the sleep physiology of healthy young adults (2007)

Despite decades of sleep research by means of polysomnography (PSG), systematic interindividual differences in PSG-assessed sleep parameters have been scarcely investigated. The present study is the first to quantify interindividual variability in standard PSG-assessed variables of sleep structure in terms of stability and robustness as well as magnitude. Twenty-one carefully screened healthy young adults were studied continuously in a strictly controlled laboratory environment, where their PSGs were recorded for eight nights interspersed with three separate 36 h sleep deprivation periods. All PSG records were scored blind to subject and condition, using conventional criteria, and delta power in the non-REM sleep EEG was computed for four electrode derivations. Interindividual differences in sleep variables were examined for stability and robustness, respectively, by comparing results across equivalent nights (e.g. baseline nights) and across experimentally differentiated nights (baseline nights versus recovery nights following sleep deprivation). Among 18 sleep variables analyzed, all except slow-wave sleep (SWS) latency were found to exhibit significantly stable and robust--i.e. trait-like--interindividual differences. This was quantified by means of intraclass correlation coefficients (ICCs), which ranged from 36% to 89% across physiologic variables, and were highest for SWS (73%) and delta power in the non-REM sleep EEG (78-89%). The magnitude of the trait interindividual differences was considerable, consistently exceeding the magnitude of the group-average effect on sleep structure of 36 h total sleep deprivation. Notably, for non-REM delta power--a putative marker of sleep homeostasis--the interindividual differences were from 9.9 to 12.8 times greater than the group-average increase following sleep deprivation relative to baseline. Physiologic sleep variables did not vary among subjects in a completely independent manner--61.1% of their combined variance clustered in three trait dimensions, which appeared to represent sleep duration, sleep intensity, and sleep discontinuity. Any independent functional significance of these sleep physiologic phenotypes remains to be determined.
Creation of a Broad-Range and Highly Stereoselectived-Amino Acid Dehydrogenase for the One-Step Synthesis ofd-Amino Acids (2006)

Using both rational and random mutagenesis, we have created the first known broad substrate range, nicotinamide cofactor dependent, and highly stereoselective d-amino acid dehydrogenase. This new enzyme is capable of producing d-amino acids via the reductive amination of the corresponding 2-keto acid with ammonia. This biocatalyst was the result of three rounds of mutagenesis and screening performed on the enzyme meso-diaminopimelate d-dehydrogenase. The first round targeted the active site of the wild-type enzyme and produced mutants that were no longer strictly dependent on the native substrate. The second and third rounds produced mutants that had an increased substrate range including straight- and branched-aliphatic amino acids and aromatic amino acids. The very high selectivity toward the d-enantiomer (95 to >99% ee) was shown to be preserved even after the addition of the five mutations found in the three rounds of mutagenesis and screening. This new enzyme could complement and improve upon current methods for d-amino acid synthesis.
Ferroelectricity in hafnium oxide thin films (2011)

We report that crystalline phases with ferroelectric behavior can be formed in thin films of SiO2 doped hafnium oxide. Films with a thickness of 10 nm and with less than 4 mol. % of SiO2 crystallize in a monoclinic/tetragonal phase mixture. We observed that the formation of the monoclinic phase is inhibited if crystallization occurs under mechanical encapsulation and an orthorhombic phase is obtained. This phase shows a distinct piezoelectric response, while polarization measurements exhibit a remanent polarization above 10 μC/cm2 at a coercive field of 1 MV/cm, suggesting that this phase is ferroelectric. Ferroelectric hafnium oxide is ideally suited for ferroelectric field effect transistors and capacitors due to its excellent compatibility to silicon technology.
Complement Regulatory Genes and Hemolytic Uremic Syndromes (2007)

Hemolytic uremic syndrome is a triad of microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure. It is one of a group of conditions termed the thrombotic microangiopathies, which are characterized by prominent endothelial cell injury. It may be diarrheal-associated or atypical (aHUS). Evidence for a pathogenic role of the alternative pathway of complement was first suggested in 1974. Mutations in the complement regulatory proteins factor H, membrane cofactor protein (CD46), and factor I predispose to aHUS development. Mutations of the activating components factor B and complement C3 have also been reported. Penetrance is approximately 50%, suggesting other genetic and environmental modifiers are needed for disease expression. Identification of mutations is important owing to differences in mortality, renal survival, and outcome of renal transplantation. Current treatment is plasma infusion/exchange, but complement inhibitor therapy provides hope for the future.
Monocytes enhance cell proliferation and LMP1 expression of nasal natural killer/T‐cell lymphoma cells by cell contact‐dependent interaction through membrane‐bound IL‐15 (2011)

Nasal natural killer (NK)/T-cell lymphoma (NNKTL) is an Epstein-Barr virus (EBV)-related malignancy with poor prognosis and has distinct histological features characterized by angiocentric and polymorphous lymphoreticular infiltrates including inflammatory cells such as granulocytes, monocytes, macrophages and lymphocytes. Here, we show that the monocytes enhance proliferation as well as LMP1 expression of NNKTL cells by cell contact-dependent interaction through membrane-bound interleukin (IL)-15. We used two EBV-positive NK-cell lines, SNK6 and KAI3, which originated from two patients-SNK6 from a patient with NNKTL and KAI3 from a patient with a severe mosquito allergy. We cocultured the cell lines with granulocytes or monocytes and examined whether proliferation, survival and LMP1 expression of the cells changed. Although cocultured granulocytes did not affect proliferation, survival or LMP1 expression of the cells, cocultured monocytes enhanced both proliferation and LMP1 expression in a dose-dependent manner. These phenomena were not seen when monocytes were placed in a separate chamber. Moreover, the monocyte-inducible proliferation and LMP1 expression were inhibited by treatment with an antibody against IL-15. Furthermore, production of interferon-gamma-inducible protein (IP)-10 were enhanced by coculture with monocytes and were inhibited by the antibody. Immunohistological studies confirmed that a number of infiltrating CD14-positive monocytes contacted CD56-positive lymphoma cells in all of 20 NNKTL tissues tested. These results suggest that monocytes enhance cell growth as well as LMP1 expression of NNKTL cells by cell contact-dependent interaction through membrane-bound IL-15. In the microenvironment of NNKTL tissue, a positive feedback loop of interaction between lymphoma cells and monocytes may be present and contribute to lymphoma progression.
Catalytic C–H Carboxylation of Terminal Alkynes with Carbon Dioxide (2012)

Within the past years, several catalyst systems have been discovered that promote the carboxylation of terminal alkynes with formation of propiolic acids. This reaction concept is particularly topical in that it involves the functionalization of a C–H bond and the incorporation of CO2 into an organic molecule without need for strong bases or aggressive organometallic reagents. The present article reviews the development of this new reaction type and critically compares the reaction and catalyst concepts disclosed in the literature.
Using the Similarity of Main Melodies to Identify Cover Versions of Popular Songs for Music Document Retrieval (2008)

Automatic extraction of information from music data is an important and challenging issue in the field of content-based music retrieval. As part of the research effort, this study presents a technique that automatically identifies cover versions of songs specified by users. The technique enables users to search for songs with an identical tune, but performed by different singers, in different languages, genres, and so on. The proposed system takes an excerpt of the song specified by the user as input, and returns a ranked list of songs similar to the input excerpt in terms of the main melody. To handle likely discrepancies, e.g., in tempo, transposition, and accompaniment, between cover versions and the original song, methods are presented to remove the non-vocal portions of the song, extract the sung notes from the accompanied vocals, and compare the similarities between the sung note sequences. Our experiments on a database of 594 cross-lingual popular songs show the feasibility of identifying cover versions of songs for music retrieval.
Site-specific Conjugation on Serine → Cysteine Variant Monoclonal Antibodies (2000)

We have engineered a cysteine residue at position 442 (EU/OU numbering) in the third constant domain (C(H)3) of the heavy chain of several IgGs with different specificities, isoforms, and variants with the intent to introduce a site for chemical conjugation. The variants were expressed in NS0 mouse myeloma cells, where monomeric IgG is the major form and formation of aggregate was minimal. Monomeric IgG contained no free thiol; however, it was discovered that the engineered thiols were reversibly blocked and could be reduced under controlled conditions. Following reduction, reactive thiol was conjugated with a cysteine-specific bifunctional chelator, bromoacetyl-TMT to a humanized 323/A3 IgG4 variant. Conjugation had no significant effect on antibody affinity. To prove that the conjugation was site-specific, an antibody-TMT conjugate was labeled with lutetium-177 and subjected to peptide mapping followed by sequence analysis. Glu-C digestion demonstrated that 91% of the label was recovered in the COOH-terminal peptide fragment containing the engineered cysteine.
Pharmacologic treatment of hyperlipidemia. (2011)

Pharmacologic treatment of hyperlipidemia in conjunction with therapeutic lifestyle changes can be used for both primary and secondary prevention of cardiovascular disease. Statins have the most convincing data for primary prevention, especially for higher risk patients. Therefore, risk stratification is essential. Statin therapy is also recommended for secondary prevention in all patients with known cardiovascular disease or the risk equivalent. High-dose statins should be initiated in patients with acute coronary syndrome. Omega-3 fatty acids may be a good alternative after myocardial infarction for patients who cannot tolerate statins. Fibrates and niacin have not been shown to reduce all-cause mortality in secondary prevention, but may be useful adjuncts when statins alone cannot adequately control lipid levels. Other cholesterol-lowering medications used for primary or secondary prevention of cardiovascular disease have not been shown to consistently improve patient-oriented outcomes. There is good evidence for using statins in the secondary prevention of stroke and peripheral arterial disease.
3.7 A fully integrated TV tuner front-end with 3.1dB NF, &#x003E;&#x002B;31dBm OIP3, &#x003E;83dB HRR3/5 and &#x003E;68dB HRR7 (2014)

In TV tuner systems, the RF front-end design has been a challenging issue since it must simultaneously satisfy over 65dB of harmonic rejection (HR), and have high linearity for high-power input and low noise over wide bandwidth (48-to-870MHz). In terms of harmonic rejection, even though the state-of-the-art work reports over 60dB rejections on the 3 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rd</sup> - and 5 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> - order harmonics with a single mixer [1], higher-than-5 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> -order harmonic rejections are still required for the low-band channels in TV tuners and thereby RF filters are indispensable at the RF front-end. However, due to the difficulties of integrating RF filters satisfying low noise and high linearity over wide bandwidth, the previous works inevitably had to use external inductors [2-4]. Although a recent work successfully integrates an RF filter satisfying all the stringent specifications by current-domain signal flow from the LNA output to the baseband stage [5], the transconductance stage at the filter input is not linear enough to drive the high-power input and thus the input signal needs to be attenuated at the RF front-end, which eventually degrades system SNR.
Thalamic Stimulation for Chronic Pain (1983)

Electrical stimulation of the thalamus was found to be effective for the treatment of chronic intractable pain of various etiologies. Diencephalic recordings revealed spontaneous focal electrical discharge patterns with relatively low activation thresholds. Localization of the optimum thalamic discharge site is used as a guide for the electrode implant to be used for chronic stimulation. The thalamic sites which most frequently displayed the low threshold spontaneous focal discharge activity were the CM-Pf complex and related intralaminar nuclear structures. Generation of diencephalic pain-reverberating circuits was discussed in relation to explaining the pain syndromes herein described. The applied electrical stimulation is thought to electrophysiologically 'jam' the low threshold discharging systems and thereby alleviate pain.
<title>Fast motion estimation using circular zonal search</title> (1998)

Block based motion estimation is widely used for exploiting temporal correlation within an image. Still the full search algorithm, which is considered to be the optimal, is computational intensive. In this paper a new fast motion estimation method for video coding, is presented. It will be shown that the new algorithm is not only much faster than traditional algorithms, but in some cases can achieve much better visual quality, even from the 'optimal' full search algorithm.
Modulation of mammalian life span by the short isoform of p53 (2004)

Overexpression of the short isoform of p53 (p44) has unexpectedly uncovered a role for p53 in the regulation of size and life span in the mouse. Hyperactivation of the insulin-like growth factor (IGF) signaling axis by p44 sets in motion a kinase cascade that clamps potentially unimpeded growth through p21Cip1. This suggests that pathways of gene activity known to regulate longevity in lower organisms are linked in mammals via p53 to mechanisms for controlling cell proliferation. Thus, appropriate expression of the short and long p53 isoforms might maintain a balance between tumor suppression and tissue regeneration, a major requisite for long mammalian life span.
A SOURCE TAGGING THEORY FOR HETEROGENEOUS DATABASE SYSTEMS (1990)

Many important Management Support Systems require seamless access to and integration of multiple heterogeneous database systems. This paper studies heterogeneous database systems from the source perspective. It aims at addressing issues such as the following: (1) Where is the data from? (2) Which intermediate data sources were used to arrive at that data? Specifically, it presents a polygen model for resolving the Data Source Tagging and Intermediate Source Tagging problems. In addition, it presents the necessary and sufficient condition for source tagging. Source knowledge is important for many reasons. It enables users to apply their own judgment to the credibility of the information. It enables users to rationalize and reconcile data inconsistencies. It enables system designers to develop access charge systems. It enables an application user to adjust data. It enables a system to interpret data semantics more accurately. In sum, it justifies having source tagging capabilities as a required functionality for future heterogeneous database systems.