gebhart/patrepeval huggingface dataset:
EGF‐like domains in extracellular matrix proteins: Localized signals for growth and differentiation? (1989)

Multidomain proteins of the extracellular matrix (ECM) play an important role in development and maintenance of cellular organization and in tissue repair. Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. The mitogenic activity of laminin is restricted to a fragment which consists of about 25 repeating domains with partial homology to EGF and comprises the rod-like inner regions of the three short arms of the four armed molecule. The mitogenic activity does not correlate with promotion of cell attachment and neurite outgrowth for which major functional sites have been found in other regions of the laminin molecule. It is suggested that EGF-like domains in laminin, in other ECM proteins and in the extracellular portions of some membrane proteins are signals for cellular growth and differentiation. Because they are integral parts of large molecules and often of supramolecular assemblies these domains are well suited to stimulate neighboring cells in a specific and vectorial way. This concept of localized growth or differentiation signals offers an attractive mechanism for the regulation of cellular development.
A wearable chemical–electrophysiological hybrid biosensing system for real-time health and fitness monitoring (2016)

Flexible, wearable sensing devices can yield important information about the underlying physiology of a human subject for applications in real-time health and fitness monitoring. Despite significant progress in the fabrication of flexible biosensors that naturally comply with the epidermis, most designs measure only a small number of physical or electrophysiological parameters, and neglect the rich chemical information available from biomarkers. Here, we introduce a skin-worn wearable hybrid sensing system that offers simultaneous real-time monitoring of a biochemical (lactate) and an electrophysiological signal (electrocardiogram), for more comprehensive fitness monitoring than from physical or electrophysiological sensors alone. The two sensing modalities, comprising a three-electrode amperometric lactate biosensor and a bipolar electrocardiogram sensor, are co-fabricated on a flexible substrate and mounted on the skin. Human experiments reveal that physiochemistry and electrophysiology can be measured simultaneously with negligible cross-talk, enabling a new class of hybrid sensing devices.
Loss of genes on the short arm of chromosome 11 in human lung carcinomas. (1987)

Since loss of genes on the short arm of chromosome 11 (11p) is known to be closely associated with several human tumors, we analyzed DNA of lung carcinomas from 45 patients with respect to loss of heterozygosity at loci on 11p. We observed that loss of heterozygosity at 11p loci was quite frequent (17 of 41 informative cases, 41%) and it appeared that the loss might be associated with more advanced carcinomas than those of stage Ia. In many cases, sequences at the CAT and/or the D11S12-IGF2 loci were commonly deleted. These observations suggest that loss of genes at particular regions on 11p might be involved in progression of human lung carcinomas.
Transformation-based Framework for Record Matching (2008)

Today's record matching infrastructure does not allow a flexible way to account for synonyms such as "Robert" and "Bob" which refer to the same name, and more general forms of string transformations such as abbreviations. We propose a programmatic framework of record matching that takes such user-defined string transformations as input. To the best of our knowledge, this is the first proposal for such a framework. This transformational framework, while expressive, poses significant computational challenges which we address. We empirically evaluate our techniques over real data.
Enoyl-acyl-carrier-protein reductase and Mycobacterium tuberculosis InhA do not conserve the Tyr-Xaa-Xaa-Xaa-Lys motif in mammalian 11 β- and 17 β-hydroxysteroid dehydrogenases and Drosophila alcohol dehydrogenase (1995)

Correspondence| August 01 1995 Enoyl-acyl-carrier-protein reductase and Mycobacterium tuberculosis InhA do not conserve the Tyr-Xaa-Xaa-Xaa-Lys motif in mammalian 11 β- and 17 β-hydroxysteroid dehydrogenases and Drosophila alcohol dehydrogenase M E Baker M E Baker Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1995) 309 (3): 1029–1030. https://doi.org/10.1042/bj3091029 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation M E Baker; Enoyl-acyl-carrier-protein reductase and Mycobacterium tuberculosis InhA do not conserve the Tyr-Xaa-Xaa-Xaa-Lys motif in mammalian 11 β- and 17 β-hydroxysteroid dehydrogenases and Drosophila alcohol dehydrogenase. Biochem J 1 August 1995; 309 (3): 1029–1030. doi: https://doi.org/10.1042/bj3091029 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1995 The Biochemical Society, London1995 Article PDF first page preview Close Modal You do not currently have access to this content.
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function. (1982)

We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6) (ampicillin resistance) gene of the plasmid pBR322. Evidence suggests that the active site for this enzyme may include a serine-threonine dyad (residues 70 and 71). By priming in vitro DNA synthesis with a chemically synthesized 16-base oligodeoxyribonucleotide, we have inverted the Ser-Thr dyad to Thr-Ser and thereby generated a mutant with an ampicillin-sensitive phenotype. This "double-mismatch" method is relatively simple and also very general because detection of mutants is at the level of DNA and involves only colony hybridization. Accordingly, the procedure can be applied to any DNA sequence and does not depend on the phenotype of the mutant.
Expression of cyclin B1 in the metaplasia‐dysplasia‐carcinoma sequence of Barrett esophagus (2001)

Overexpression of cyclin B1 is a frequent and early finding in the metaplasia-dysplasia-carcinoma sequence in BE. It may contribute to the loss of growth control and, subsequently, to the development of tumors in this location.
Integrin αVβ3 contains a receptor site for resveratrol (2006)

Resveratrol is a naturally occurring polyphenol, which causes apoptosis in cultured cancer cells. We describe a cell surface resveratrol receptor on the extracellular domain of hetero-dimeric alphaVbeta3 integrin in MCF-7 human breast cancer cells. This receptor is linked to induction by resveratrol of extracellular-regulated kinases 1 and 2 (ERK1/2)- and serine-15-p53-dependent phosphorylation leading to apoptosis. The integrin receptor is near the Arg-Gly-Asp (RGD) recognition site on the integrin; an integrin-binding RGD peptide inhibits induction by resveratrol of ERK1/2- and p53-dependent apoptosis. Antibody (Ab) to integrin alphaVbeta3, but not to alphaVbeta5, inhibits activation by resveratrol of ERK1/2 and p53 and consequent apoptosis in estrogen receptor-alpha (ERalpha) positive MCF-7, and ERalpha-negative MDA-MB231 cells. Resveratrol is displaced from the purified integrin by an RGD, but not RGE, peptide, and by alphaVbeta3 integrin-specific Ab. Resveratrol action is blocked by siRNAbeta3, but not by siRNAalphaV. [14C]-Resveratrol binds to commercially purified integrin alphaVbeta3 and to alphaVbeta3 prepared from MCF-7 cells; binding of [14C]-resveratrol to the beta3, but not to the alphaV monomer, is displaced by unlabeled resveratrol. In conclusion, binding of resveratrol to integrin alphaVbeta3, principally to the beta3 monomer, is essential for transduction of the stilbene signal into p53-dependent apoptosis of breast cancer cells.
Complement drives Th17 cell differentiation and triggers autoimmune arthritis (2010)

Activation of serum complement triggers Th17 cell-dependent spontaneous autoimmune disease in an animal model. In genetically autoimmune-prone SKG mice, administration of mannan or beta-glucan, both of which activate serum complement, evoked Th17 cell-mediated chronic autoimmune arthritis. C5a, a chief component of complement activation produced via all three complement pathways (i.e., lectin, classical, and alternative), stimulated tissue-resident macrophages, but not dendritic cells, to produce inflammatory cytokines including IL-6, in synergy with Toll-like receptor signaling or, notably, granulocyte/macrophage colony-stimulating factor (GM-CSF). GM-CSF secreted by activated T cells indeed enhanced in vitro IL-6 production by C5a-stimulated macrophages. In vivo, C5a receptor (C5aR) deficiency in SKG mice inhibited the differentiation/expansion of Th17 cells after mannan or beta-glucan treatment, and consequently suppressed the development of arthritis. Transfer of SKG T cells induced Th17 cell differentiation/expansion and produced arthritis in C5aR-sufficient recombination activating gene (RAG)-/- mice but not in C5aR-deficient RAG-/- recipients. In vivo macrophage depletion also inhibited disease development in SKG mice. Collectively, the data suggest that complement activation by exogenous or endogenous stimulation can initiate Th17 cell differentiation and expansion in certain autoimmune diseases and presumably in microbial infections. Blockade of C5aR may thus be beneficial for controlling Th17-mediated inflammation and autoimmune disease.
A new macrocyclic antibiotic, fidaxomicin (OPT-80), causes less alteration to the bowel microbiota of Clostridium difficile-infected patients than does vancomycin (2010)

Clostridium difficile infection (CDI) is the most common identifiable cause of diarrhoea in hospitalized patients. Current therapies rely on the administration of metronidazole or vancomycin, which reduce vegetative populations of C. difficile in the bowel. Recurrence of the disease when treatment with these antibiotics ceases indicates that metronidazole and vancomycin affect not only C. difficile but also commensal populations that normally mediate competitive exclusion. Fidaxomicin is a new antibiotic that inhibits C. difficile. Our study shows that fidaxomicin had little effect on the composition of the faecal microbiota in terms of its major phylogenetic clusters. Notably, clostridial clusters XIVa and IV, and Bifidobacterium, were much less affected by fidaxomicin compared to vancomycin treatment. These findings help to explain the substantially reduced rates of relapse following treatment of CDI with fidaxomicin in recent clinical trials.
Immunohistochemical Distinction of Invasive From Noninvasive Breast Lesions (2003)

Identification of myoepithelial cells using antibodies to cytoskeletal proteins, such as smooth muscle myosin heavy chain (SMM-HC) and calponin, can play an important role in distinguishing invasive carcinoma from its histologic mimics. However, antibodies to these proteins may also cross-react with stromal myofibroblasts and vascular smooth muscle cells. It has recently been demonstrated that myoepithelial cells express the nuclear protein, p63, a member of the p53 gene family. We compared the patterns of reactivity of antibodies with p63, calponin, and SMM-HC on 85 breast lesions, including 11 cases of sclerosing adenosis, 33 cases of ductal carcinoma in situ, including 10 that showed microinvasion, 6 cases of lobular carcinoma in situ, and 35 cases of infiltrating ductal carcinoma. All three antibodies were positive on the vast majority of myoepithelial cells in all cases. A small minority of cases showed focal gaps in the revealed myoepithelial cell layer, reflected in discontinuous positive immunostaining around noninvasive epithelial nests (including ductal carcinoma in situ). No case showed p63 expression by myofibroblasts or vascular smooth muscle cells, whereas myofibroblasts expressed, in 8% and 76% of cases, SMM-HC and calponin, respectively. Although no tumor cell reactivity was noted with antibodies to calponin or SMM-HC, tumor cells in 11% of cases showed at least focal p63 expression. And although antibodies to p63 offer excellent sensitivity and increased specificity for myoepithelial detection relative to antibodies to calponin and SMM-HC, they have the following diagnostic limitations: 1) they occasionally demonstrate an apparently discontinuous myoepithelial layer, particularly around ductal carcinoma in situ, and 2) they react with a small but significant subset of breast carcinoma tumor cells. p63 may represent a myoepithelial marker that can complement or replace SMM-HC and/or calponin in the analysis of difficult breast lesions.
Burkitt's Lymphoma: Molecular Pathogenesis and Treatment (2000)

AbstractDennis Burkitt first described a unique form of sarcoma involving the jaw of African children in 1958 (1). Before long, Burkitt's lymphoma became an accepted distinct pathologic and clinical diagnosis, and over the ensuing 30 years, much has been learned about the molecular basis of this highly aggressive non-Hodgkin's lymphoma (NHL). This disease has been the subject of multiple successful scientific and clinical studies (2–6) and serves as a model of oncogenesis in malignant lymphomas. Indeed, the most exciting developments in the study of Burkitt's lymphoma come from research on the molecular pathogenesis of this highly aggressive albeit curable lymphoma. Clinically, the incremental and rational development of treatment regimens over the past 20 years has led to the cure of most patients with Burkitt's lymphomas (2–6). The success of these regimens, however, has not been achieved without significant toxicity, and further refinements are needed to reduce toxicity and to overcome drug resistance in the few incurable patients.
Colocalization of muscleblind with RNA foci is separable from mis-regulation of alternative splicing in myotonic dystrophy (2005)

Myotonic dystrophy type I (DM1), which is caused by a non-coding CTG-repeat expansion in the dystrophia myotonica-protein kinase (DMPK) gene, is an RNA-mediated disease. Expanded CUG repeats in transcripts of mutant DMPK form nuclear foci that recruit muscleblind-like (MBNL) proteins, a family of alternative splicing factors. Although transcripts of mutant DMPK and MBNL proteins accumulate in nuclear RNA foci, it is not clear whether foci formation is required for splicing mis-regulation. Here, we use a co-transfection strategy to show that both CUG and CAG repeats form RNA foci that colocalize with green fluorescent protein (GFP)-MBNL1 and endogenous MBNL1. However, only CUG repeats alter splicing of the two tested pre-mRNAs, cardiac troponin T (cTNT) and insulin receptor (IR). Using FRAP, we demonstrate that GFP-MBNL1 in CUG and CAG foci have similar half-times of recovery and fractions of immobile molecules, suggesting that GFP-MBNL1 is bound by both CUG and CAG repeats. We also find an immobile fraction of GFP-MBNL1 in DM1 fibroblasts and a similar rapid exchange in endogenous CUG RNA foci. Therefore, formation of RNA foci and disruption of MBNL1-regulated splicing are separable events.
Television production handbook (1961)

This classic, best-selling text introduces students to the basic skills required in all aspects of television production, including camera and studio, field equipment and production, and multicamera directing, all while emphasizing the latest production techniques and technology, such as audio workstations, non-linear editing, and HDTV. This text introduces cutting edge developments in the field, while maintaining its name as the reference text for the TV Production course. This is the most current, technically accurate text available and offers the most extensive teaching and learning package. Zettl's TELEVISION PRODUCTION HANDBOOK is the reference text and standard for the course.
Expression of Transforming Growth Factor-β1, Activin A, and Their Receptors in Thyroid Follicle Cells: Negative Regulation of Thyrocyte Growth and Function1 (1999)

Thyroid growth and function are intricately regulated by both positive and negative factors. In the present study, we have investigated the expression of transforming growth factor-beta (TGF-beta) super-family members and their receptors in normal porcine thyroid follicle cells. In tissue sections of porcine thyroids, we observed an expression of TGF-beta1, activin A, and bone morphogenetic protein (BMP)-7 proteins. The staining was localized to the follicular epithelium. In affinity cross-linking experiments, TGF-beta1 was found to bind to heteromeric complexes of TGF-beta type I and type II receptors, and activin A bound most efficiently to heteromeric complexes of activin type IB and type II receptors. We were unable to detect any BMP receptors (BMPRs) in attempts to perform affinity cross-linking with BMP-7. However, expression of BMPR-IA and BMPR-II messenger RNA (mRNA) was detected by Northern blot analysis. Both TGF-beta1 and activin A, but not BMP-7, increased the phosphorylation of Smad2, induced nuclear translocation of Smad2, Smad3, and Smad4, and inhibited thyrocyte cell growth as well as TSH-stimulated cAMP response. TGF-beta1 was more potent, compared with activin A, to induce these cellular responses. Taken together, our findings indicate a role for several members of the TGF-beta family in regulation of thyroid growth and function.
ChemInform Abstract: C‐GLYCOSYL DERIVATIVES. XLII. SYNTHESIS OF NOVEL TYPES OF C‐GLYCOSYL DERIVATIVES FROM ACETYLENIC SUGARS OR THEIR PARTIAL SYNTHETIC EQUIVALENTS. PRELIMINARY COMMUNICATION (1982)

Abstract Neue Typen von C‐Glycosylverbindungen, die mitunter strukturell mit natürlichen Nucleosiden verwandt sind, werden synthetisiert.
Static-, Dynamic-, and Mixed-Eccentricity Fault Diagnoses in Permanent-Magnet Synchronous Motors (2009)

Mixed-eccentricity (ME) fault diagnosis has not been so far documented for permanent-magnet (PM) synchronous motors (PMSMs). This paper investigates how the static eccentricity (SE), dynamic eccentricity (DE), and ME in three-phase PMSMs can be detected. A novel index for noninvasive diagnosis of these eccentricities is introduced for a faulty PMSM. The nominated index is the amplitude of sideband components with a particular frequency pattern which is extracted from the spectrum of stator current. Using this index makes it possible to determine the occurrence, as well as the type and percentage, of eccentricity precisely. Meanwhile, the current spectrum of the faulty PMSM during a large span is inspected, and the ability of the proposed index is exhibited to detect eccentricity in faulty PMSMs with different loads. A novel theoretical scrutiny based on a magnetic field analysis is presented to prove the introduced index and generalize the illustrated fault recognition method. To show the merit of this index in the eccentricity detection and estimation of its severity, first, the correlation between the index and the SE and DE degrees is determined. Then, the type of the eccentricity is determined by a <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</i> -nearest neighbor classifier. At the next step, a three-layer artificial neural network is employed to estimate the eccentricity degree and its type. After all, a white Gaussian noise is added to the simulated current, and the robustness of the proposed index is analyzed with respect to the noise variance. In this paper, the PMSM under magnetic fault (demagnetization) and electrical faults (short and open circuits) is modeled, and the current spectrum of the faulty PMSM under demagnetization, short circuit, and open circuit faults is analyzed. It is demonstrated that the proposed index, due to eccentricity fault, is not generated in the current spectrum due to magnetic and electrical faults. Indeed, it is exposed that the introduced index is only created due to eccentricity fault and it is not sensitive to other faults. To model the PMSM eccentricities, a time-stepping finite-element method, which takes into account all geometrical and physical characteristics of the machine components, nonuniform permeance of the air gap, and nonuniform characteristics of the PM material, is employed. This model facilitates the access to the demanded signals in order to have accurate processing. A comparison of simulation and experimental results validate the proposed index.
Thermal stability of magnetic tunneling junctions with MgO barriers for high temperature spintronics (2006)

Thermal stability of MgO-based magnetic tunnel junctions has been investigated from room temperature up to 500°C, in both the memory and sensor configurations. Junctions showed magnetoresistances of over 200% at room temperature and over 100% at 300°C. Below 375°C, the resistance of the parallel state remains constant, while the antiparallel state resistance linearly decreases with temperature. Above that, a rapid increase in the resistance of both states was observed, along with an irreversible loss of magnetoresistance. Junctions in the sensor configuration exhibited a constant sensitivity of 1.0%/Oe at temperatures up to 300°C before getting degraded.
Zebedee: Design of a Spring-Mounted 3-D Range Sensor with Application to Mobile Mapping (2012)

Three-dimensional perception is a key technology for many robotics applications, including obstacle detection, mapping, and localization. There exist a number of sensors and techniques for acquiring 3-D data, many of which have particular utility for various robotic tasks. We introduce a new design for a 3-D sensor system, constructed from a 2-D range scanner coupled with a passive linkage mechanism, such as a spring. By mounting the other end of the passive linkage mechanism to a moving body, disturbances resulting from accelerations and vibrations of the body propel the 2-D scanner in an irregular fashion, thereby extending the device's field of view outside of its standard scanning plane. The proposed 3-D sensor system is advantageous due to its mechanical simplicity, mobility, low weight, and relatively low cost. We analyze a particular implementation of the proposed device, which we call Zebedee, consisting of a 2-D time-of-flight laser range scanner rigidly coupled to an inertial measurement unit and mounted on a spring. The unique configuration of the sensor system motivates unconventional and specialized algorithms to be developed for data processing. As an example application, we describe a novel 3-D simultaneous localization and mapping solution in which Zebedee is mounted on a moving platform. Using a motion capture system, we have verified the positional accuracy of the sensor trajectory. The results demonstrate that the six-degree-of-freedom trajectory of a passive spring-mounted range sensor can be accurately estimated from laser range data and industrial-grade inertial measurements in real time and that a quality 3-D point cloud map can be generated concurrently using the same data.