gebhart/patrepeval huggingface dataset:
Needleless Vaccine Delivery Using Micro-Shock Waves (2011)

Shock waves are one of the most efficient mechanisms of energy dissipation observed in nature. In this study, utilizing the instantaneous mechanical impulse generated behind a micro-shock wave during a controlled explosion, a novel nonintrusive needleless vaccine delivery system has been developed. It is well-known that antigens in the epidermis are efficiently presented by resident Langerhans cells, eliciting the requisite immune response, making them a good target for vaccine delivery. Unfortunately, needle-free devices for epidermal delivery have inherent problems from the perspective of the safety and comfort of the patient. The penetration depth of less than 100 μm in the skin can elicit higher immune response without any pain. Here we show the efficient utilization of our needleless device (that uses micro-shock waves) for vaccination. The production of liquid jet was confirmed by high-speed microscopy, and the penetration in acrylamide gel and mouse skin was observed by confocal microscopy. Salmonella enterica serovar Typhimurium vaccine strain pmrG-HM-D (DV-STM-07) was delivered using our device in the murine salmonellosis model, and the effectiveness of the delivery system for vaccination was compared with other routes of vaccination. Vaccination using our device elicits better protection and an IgG response even at a lower vaccine dose (10-fold less) compared to other routes of vaccination. We anticipate that our novel method can be utilized for effective, cheap, and safe vaccination in the near future.
Automating mimicry attacks using static binary analysis (2005)

Intrusion detection systems that monitor sequences of system calls have recently become more sophisticated in defining legitimate application behavior. In particular, additional information, such as the value of the program counter and the configuration of the program’s call stack at each system call, has been used to achieve better characterization of program behavior. While there is common agreement that this additional information complicates the task for the attacker, it is less clear to which extent an intruder is constrained. In this paper, we present a novel technique to evade the extended detection features of state-of-the-art intrusion detection systems and reduce the task of the intruder to a traditional mimicry attack. Given a legitimate sequence of system calls, our technique allows the attacker to execute each system call in the correct execution context by obtaining and relinquishing the control of the application’s execution flow through manipulation of code pointers. We have developed a static analysis tool for Intel x86 binaries that uses symbolic execution to automatically identify instructions that can be used to redirect control flow and to compute the necessary modifications to the environment of the process. We used our tool to successfully exploit three vulnerable programs and evade detection by existing state-of-the-art system call monitors. In addition, we analyzed three real-world applications to verify the general applicability of our techniques.
Fabrication of Si microstructures using focused ion beam implantation and reactive ion etching (2008)

Localized Ga implantation by means of a focused ion beam and subsequent deep reactive ion etching is used to fabricate microstructures or nanostructures in Si. It is found that the area irradiated with the focused ion beam acts as an etch stop in reactive ion etching. The etch rate ratio between the unimplanted and Ga-implanted area increases to 2.56 with an increasing Ga ion dose up to 1.59 × 1016 ions cm−2. However, the etch rate ratio decreases with further increase in ion dose and the surface of the etched Si microstructures is concave due to the effect of ion sputtering during focused ion irradiation. This technique is promising for the fabrication of 3D nanostructures in Si.
Characterization of a variant of the polysaccharide acetan produced by a mutant of Acetobacter xylinum strain CR1/4 (1993)

Acetobacter xylinum NRRL B42 (NCIB 40123) produces both cellulose and a complex anionic branched heteropolysaccharide called acetan. Chemical mutagenesis was used to isolate stable cellulose-minus Acetobacter xylinum mutants. Further chemical mutagenesis of these cellulose-minus A. xylinum bacteria was used to select mutants which secrete polysaccharides which are variants of the acetan structure. Preparation, purification and characterization of these polysaccharides are described. Methylation analysis of the polysaccharide structure CR1/4 suggests that the polysaccharide has an acetan structure with a truncated sidechain terminating in glucuronic acid.
The Effect of a Golden Hour Policy on the Morbidity and Mortality of Combat Casualties (2015)

A mandate made in 2009 by Secretary of Defense Gates reduced the time between combat injury and receiving definitive care. Prehospital transport time and treatment capability are important factors for casualty survival on the battlefield.
Access to Poly{N‐[3‐(dimethylamino)propyl](meth)acrylamide} via Microwave‐Assisted Synthesis and Control of LCST‐Behavior in Water (2007)

Abstract In the present paper, the microwave (MW)‐assisted synthesis of the alkaline N ‐[3‐(dimethylamino)propyl]methacrylamide ( 4 ) and ‐acrylamide ( 5 ) monomers within minutes is described. The reaction was carried out by mixing and subsequent irradiation of 3‐dimethylamino‐1‐propylamine ( 3 ) and (meth)acrylic acid ( 1 / 2 ) without addition of solvent. After polymerization, the obtained homopolymers ( 6 / 7 ) exhibit lower critical solution temperature (LCST) behavior in water at 35 °C only at pH = 14. The LCST‐behavior can be influenced by N ‐oxidation of the tertiary amino group with hydrogen peroxide or by formation of a polymer‐inclusion complex ( 6a ) of 6 and β ‐cyclodextrin ( β ‐CD) by addition of randomly‐methylated β ‐CD. magnified image
Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortus (2006)

Phosphatidylcholine (PC) is a typical eukaryotic phospholipid absent from most prokaryotes. Thus, its presence in some intracellular bacteria is intriguing as it may constitute host mimicry. The role of PC in Brucella abortus was examined by generating mutants in pcs (BApcs) and pmtA (BApmtA), which encode key enzymes of the two bacterial PC biosynthetic routes, the choline and methyl-transferase pathways. In rich medium, BApcs and the double mutant BApcspmtA but not BApmtA displayed reduced growth, increased phosphatidylethanolamine and no PC, showing that Pcs is essential for PC synthesis under these conditions. In minimal medium, the parental strain, BApcs and BApmtA showed reduced but significant amounts of PC suggesting that PmtA may also be functional. Probing with phage Tb, antibiotics, polycations and serum demonstrated that all mutants had altered envelopes. In macrophages, BApcs and BApcspmtA showed reduced ability to evade fusion with lysosomes and establish a replication niche. In mice, BApcs showed attenuation only at early times after infection, BApmtA at later stages and BApcspmtA throughout. The results suggest that Pcs and PmtA have complementary roles in vivo related to nutrient availability and that PC and the membrane properties that depend on this typical eukaryotic phospholipid are essential for Brucella virulence.
Gold Nanorods:  Electrochemical Synthesis and Optical Properties (1997)

Aqueous solutions containing a high yield of suspended gold nanorods have been successfully synthesized via an electrochemical method. The control of preparing gold nanorods with different aspect ratios can be attained. Their absorption spectral features show a dominant surface plasma band corresponding to the longitudinal resonance, SPl, and its λmax shifts markedly to the red as the aspect ratio is increased. Meanwhile, the dependence of λmax for longitudinal resonance on the mean aspect ratio shows a deviation from the classical electrostatic model prediction at mean aspect ratios around 4 ± 1, where it limits the validity of the classical electrostatic approximation.
Constitutive Activation of NF-κB during Progression of Breast Cancer to Hormone-Independent Growth (1997)

Breast cancers often progress from a hormone-dependent, nonmetastatic, antiestrogen-sensitive phenotype to a hormone-independent, antiestrogen- and chemotherapy-resistant phenotype with highly invasive and metastatic growth properties. This progression is usually accompanied by altered function of the estrogen receptor (ER) or outgrowth of ER-negative cancer cells. To understand the molecular mechanisms responsible for metastatic growth of ER-negative breast cancers, the activities of the transcription factor NF-kappaB (which modulates the expression of genes involved in cell proliferation, differentiation, apoptosis, and metastasis) were compared in ER-positive (MCF-7 and T47-D) and ER-negative (MDA-MB-231 and MDA-MB-435) human breast cancer cell lines. NF-kappaB, which is usually maintained in an inactive state by protein-protein interaction with inhibitor IkappaBs, was found to be constitutively active in ER-negative breast cancer cell lines. Constitutive DNA binding of NF-kappaB was also observed with extracts from ER-negative, poorly differentiated primary breast tumors. Progression of the rat mammary carcinoma cell line RM22-F5 from an ER-positive, nonmalignant phenotype (E phenotype) to an ER-negative, malignant phenotype (F phenotype) was also accompanied by constitutive activation of NF-kappaB. Analysis of individual subunits of NF-kappaB revealed that all ER-negative cell lines, including RM22-F5 cells of F phenotype, contain a unique 37-kDa protein which is antigenically related to the RelA subunit. Cell-type-specific differences in IkappaB alpha, -beta, and -gamma were also observed. In transient-transfection experiments, constitutive activity of an NF-kappaB-dependent promoter was observed in MDA-MB-231 and RM22-F5 cells of F phenotype, and this activity was efficiently repressed by cotransfected ER. Since ER inhibits the constitutive as well as inducible activation function of NF-kappaB in a dose-dependent manner, we propose that breast cancers that lack functional ER overexpress NF-kappaB-regulated genes. Furthermore, since recent data indicate that NF-kappaB protects cells from tumor necrosis factor alpha-, ionizing radiation-, and chemotherapeutic agent daunorubicin-mediated apoptosis, our results provide an explanation for chemotherapeutic resistance in ER-negative breast cancers.
Utility of Free Level Monitoring of Antiepileptic Drugs (1985)

Criteria that were developed for monitoring free (unbound) rather than total (free plus bound) concentrations of antiepileptic drugs include extensive and variable binding to plasma proteins. Phenytoin and valproic acid belong to this category. It is shown that free drug concentration is independent of total drug concentration, whereas total drug concentration depends on free concentration and free fraction. Because antiepileptic drugs are predominantly bound to albumin, free fraction will increase in the presence of hypoalbuminemia (hepatic and renal disease, burns, and pregnancy). Free fraction also increases because of saturable binding (valproic acid) and competitive binding (valproic acid displacing phenytoin). There is suggestive evidence that side effects may be more closely related to the free, rather than to the total, plasma concentration of phenytoin. The clinical evidence that side effects or therapeutic effects are better correlated to the free, rather than the total, concentration of valproic acid or carbamazepine is not yet convincing. Knowledge of the free concentration improves our understanding of therapeutic and toxic effects of low total plasma concentrations. Further clinical trials are necessary for definitive assessment of the clinical relevance for free drug monitoring of valproic acid, carbamazepine, and phenytoin in the management of epileptic patients.
Discipline or profession: Anthropology and its guilds (1991)

Chambers, Erve. Applied Anthropology: A Practical Guide. Englewood Cliffs, New Jersey: Prentice Hall, 1986. ix + 258 pp., including references and index. Driben, Paul. Aroland Is Our Home: An Incomplete Victory in Applied Anthropology. New York: AMS Press, 1986. xiv + 185 pp., including chapter notes and index. $32.50 cloth. Eddy, Elizabeth M. and William L. Partridge, eds. Applied Anthropology in America, Second Edition. New York: Columbia University Press, 1987. xiii + 571 pp., including bibliography. Van Willigen, John. Applied Anthropology: An Introduction. South Hadley, Massachusetts: Bergin & Garvey Publishers, 1986. xviii + 259 pp., including bibliography and index. $36.95 cloth, $18.95 paper.
Nitric Oxide: A Physiologic Messenger (1994)

Nitric oxide is a simple molecule with many physiologic roles in the cardiovascular, neurologic, and immune systems. Although the general principles of nitric oxide synthesis are known, further research is necessary to determine what role it plays in causing disease.
Neural correlates of change in major depressive disorder anhedonia following open-label ketamine (2015)

Anhedonia is a cardinal symptom of major depression and is often refractory to standard treatment, yet no approved medication for this specific symptom exists. In this exploratory re-analysis, we assessed whether administration of rapid-acting antidepressant ketamine was associated specifically with reduced anhedonia in medication-free treatment-refractory patients with major depressive disorder in an open-label investigation. Additionally, participants received either oral riluzole or placebo daily beginning 4 hours post-infusion. A subgroup of patients underwent fluorodeoxyglucose positron emission tomography scans at baseline (1-3 days pre-infusion) and 2 hours post-ketamine infusion. Anhedonia rapidly decreased following a single ketamine infusion; this was sustained for up to three days, but was not altered by riluzole. Reduced anhedonia correlated with increased glucose metabolism in the hippocampus and dorsal anterior cingulate cortex (dACC) and decreased metabolism in the inferior frontal gyrus and orbitofrontal cortex (OFC). The tentative relationship between change in anhedonia and glucose metabolism remained significant in dACC and OFC, and at trend level in the hippocampus, a result not anticipated, when controlling for change in total depression score. Results, however, remain tenuous due to the lack of a placebo control for ketamine. In addition to alleviating overall depressive symptoms, ketamine could possess anti-anhedonic potential in major depressive disorder, which speculatively, may be mediated by alterations in metabolic activity in the hippocampus, dACC and OFC.
Relaxation Processes in Si / Si1 − x Ge x Strained Layer Superlattices: A Study by Raman Spectroscopy and X‐Ray Diffractometry (1989)

A series of superlattice structures have been studied by Raman spectroscopy and x‐ray diffractometry. The periodicity of the superlattices, the alloy composition, and the degree of relaxation have been measured. The degree of relaxation as a function of thickness for superlattices was found to be less than that for single layers. This effect has been discussed in terms of the alternating strain fields in the relaxed superlattice and related to the mechanism of the relaxation process.
Choice of Spatial Frequency for Contrast Sensitivity Evaluation After Corneal Refractive Surgery (2001)

Contrast sensitivity measurements at 6 and 12 c/deg appear to be most useful in the assessment of patients who have undergone laser refractive surgery because defocus and optical aberrations primarily affect the higher spatial frequencies.
Demonstration of Blue and Green GaN-Based Vertical-Cavity Surface-Emitting Lasers by Current Injection at Room Temperature (2011)

We realized room-temperature lasing of blue and green GaN-based vertical-cavity surface-emitting lasers (VCSELs), for the first time, by current injection. The blue GaN-based VCSEL had a threshold current of 1.5 mA and a threshold voltage of 3.3 V under continuous-wave operation. Its maximum output power was 0.70 mW and its laser emission wavelength was 451 nm. The green GaN-based VCSEL had a threshold current of 22 mA and a threshold voltage of 6.3 V under pulsed current operation. Its maximum output power was estimated to be over 0.80 mW and the laser emission wavelength was 503 nm.
Nitroxyl inhibits breast tumor growth and angiogenesis (2007)

Nitroxyl (HNO) can inhibit the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Because of the importance of glycolysis in many malignant cells, we thus propose that HNO can adversely affect tumor growth. This hypothesis was tested using in vitro and in vivo models of breast cancer. We report here for the first time that HNO suppresses the proliferation of both estrogen receptor (ER)-positive and ER-negative human breast cancer cell lines, in a dose dependent manner. Mice treated with HNO either injected into the tumor itself or via the intraperitoneal approach had smaller xenograft tumor size. In addition to significantly decreased blood vessel density in the HNO-treated tumors, we observed lower levels of circulating serum vascular endothelial growth factor (VEGF). Accordingly, there was a decrease in total HIF-1alpha (hypoxia-inducible factor) protein in HNO-treated tumor cells. Further studies showed inhibition of GAPDH activity in HNO-treated human breast cancer cell lines and in HNO-treated tumor tissue derived from xenografts. One explanation for the multiplicity of actions observed after HNO treatment could be the effect from the initial inhibition of GAPDH, providing a potential therapeutic avenue based upon blocking glycolysis resulting in decreased HIF-1alpha, thus leading to angiogenesis inhibition. Therefore, HNO appears to act via mechanism(s) different from those of existing breast cancer drugs, making it a potential candidate to overcome known and emerging drug resistance pathways.
Physico-Chemical Characterization of Drugs and Drug Forms in the Solid State (2003)

This review addresses the general problem of the physico-chemical characterization of drugs and drug products. The characterization may be performed with a range of methods that appears to be mostly limited by imagination, and aims to fulfill one or more of the following goals: gather data relevant in predicting / optimizing performances of a drug, selecting its delivery system, planning a scaled-up production and associated quality controls, complying with regulations. In keeping with the general focus of the Journal, most examples presented will deal with antinfective agents, although the physico-chemical characterization is largely independent of the therapeutic goal. We begin by reviewing the substantial and recent advances in new classes of materials for drug delivery and in techniques for experimental and theoretical studies of physico-chemical properties of pharmaceutical compounds. We then introduce through examples the complex phenomenology of these properties, which may be investigated also with standard and inexpensive experimental techniques. The last part of the review addresses the problem of defining a fast and reliable protocol for investigating drug-excipient compatibility problems. Keywords: physico-chemical characterization, drugs, scaled-up production, drug-excipient compatibility, drug, &, excipient system
Carbohydrate and phenolic constituents in a comprehensive range of rapeseed and canola fractions: Nutritional significance for animals (1984)

Abstract Cotyledons from 11 varieties of rapeseed and canola seed were extracted with hexane and analysed for tannins, α‐amylase inhibition value, starch and available carbohydrate by conventional methods. The sinapine content (total sinapic acid esters plus free sinapic acid) was determined by a spectrophotometric method. Samples of soya bean cotyledons, high‐tannin sorghum grain and commercial rapeseed and canola meals were also analysed for comparison. Sinapine in rapeseed and canola cotyledons was measured at mean levels of 2.67 and 2.85% respectively. Tannins were detected in these cotyledons but only at low levels. The α‐amylase inhibition values were also low or negative. Starch was detected in negligible amounts, and available carbohydrate at 13–14%. The predicted metabolisable energy (ME) value of commercial rapeseed or canola meal for poultry was found to be numerically close to the determined value when the available carbohydrate value was reduced to 35% of the observed value.
Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers (2012)

Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL(-1) of blood in 80% of patients with metastatic prostate cancer (n = 20), in 70% of patients with metastatic breast cancer (n = 30), in 50% of patients with metastatic pancreatic cancer (n = 18), and in 0% of normal controls (n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.
Fornix-Dependent Induction of Hippocampal CCAAT Enhancer-Binding Protein β and δ Co-Localizes with Phosphorylated cAMP Response Element-Binding Protein and Accompanies Long-Term Memory Consolidation (2001)

The cAMP response element-binding protein (CREB) is an evolutionarily conserved transcription regulator essential for long-term memory formation. It is not known, however, whether the molecular events downstream of CREB activation are also conserved. An early, cAMP-dependent event necessary for learning-related long-term synaptic plasticity in the invertebrate Aplysia californica is the induction of the transcription factor CCAAT enhancer-binding protein (C/EBP). Here we show that two homologs in the rat, C/EBPbeta and C/EBPdelta, are induced at discrete times after inhibitory avoidance learning and co-localize with phosphorylated CREB in the hippocampus. This induction is blocked by fornix lesions, which are known to disrupt activation of CREB in the hippocampus and to impair memory consolidation. These results indicate that C/EBPs are evolutionarily conserved components of the CREB-dependent gene cascade activated in long-term memory.
Chromatographische Racemattrennung (1980)

Racemic thalidomide is chromatographically separated on optically active polyamide giving both enantiomers. After i.p. administration only the S-(-)-enantiomer is teratogenic on SWS mice and Natal rats.
COMPARATIVE CYTOTOXICITY POTENTIAL OF SOFT CONTACT LENS CARE PRODUCTS (2005)

Assays of HCE-T cell viability by MTS/PES, and cell membrane integrity by NRUR cells yielded data that were similar to that previously reported with mouse L929 cells in tests based on the USP Elution Test. For the MTS/PES and NRUR assay methods, the solutions in order of increasing cytotoxicity potential were: SOLOCare = COMPLETE Comfort Plus < ReNu < < OPTI-FREE Express with Aldox.
The Fas Death Factor (1995)

Fas ligand (FasL), a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis of Fas-bearing cells. Various cells express Fas, whereas FasL is expressed predominantly in activated T cells. In the immune system, Fas and FasL are involved in down-regulation of immune reactions as well as in T cell-mediated cytotoxicity. Malfunction of the Fas system causes lymphoproliferative disorders and accelerates autoimmune diseases, whereas its exacerbation may cause tissue destruction.