gebhart/patrepeval huggingface dataset:
Localization and interaction of the cis-acting elements for abscisic acid, VIVIPAROUS1, and light activation of the C1 gene of maize. (1996)

The C1 regulatory gene of the maize anthocyanin pathway is regulated by a combination of developmental and environmental signals that include the Viviparous1 (Vp1) gene, abscisic acid (ABA), and light. Using protoplast electroporation and particle bombardment assays, we have defined c/s-acting elements that are necessary and sufficient for the activation of C1 by ABA, VP1, and light, respectively. The sequence from positions -142 to -132 (CGTCCATGCAT) is essential for VP1 activation, whereas a larger overlapping element from -147 to -132 (CGTGTCGTCCATGCAT) is necessary and sufficient for activation by ABA. A separate light (blue and red)-responsive c/s element is located between positions -116 and -59. Light interacts synergistically with the ABA and VP1 responses in transient expression assays, suggesting that combinatorial interaction between modules plays a role in integrating these signals in the developing seed.
A novel data calibration scheme for electrical impedance tomography (2003)

In electrical impedance tomography surface measurements of voltages and currents are recorded and the image reconstruction algorithm uses this set of boundary data to estimate internal electrical properties of the region under investigation. Therefore correct and accurate modelling of the current and voltage distributions (forward model) is an essential part of any reconstruction method. In this paper, we explored the root cause of a boundary layer effect in the reconstructed conductivity map and found it to be an artefact arising from 2D to 3D data-model mismatch within the imaging algorithm. We propose a data calibration scheme that improves the reconstruction results by removing these boundary or edge effects. We present both two-dimensional and three-dimensional images for agar phantoms using this data calibration scheme which are markedly better than their counterparts recovered when the measurement data are not calibrated with the procedure outlined herein.
Community Health Assessment by Local Health Departments (2013)

Using the data from the National Association of County and City Health Officials’ (NACCHO) 2010 Profile of Local Health Departments (LHDs) our study investigates whether or not infrastructural characteristics of LHDs were associated with completion of community health assessment (CHA). Our results show that local and shared LHD governance, greater share of revenue from federal and state sources, smaller population size in LHD jurisdiction, and having an epidemiologist significantly increased the odds of CHA completion in the past, after controlling for community characteristics and other independent variables. These findings have important implications for LHDs, PHAB and its partners.
R‐(+)‐HA‐966, a glycine/NMDA receptor antagonist, selectively blocks the activation of the mesolimbic dopamine system by amphetamine (1991)

1. The effects of the glycine/NMDA receptor antagonist, (+)-HA-966 on the neurochemical and behavioural responses to amphetamine have been determined in the mouse and rat. 2. In vehicle-treated control mice, (+)-HA-966 (30-100 mg kg-1) did not affect dopamine synthesis in either the nucleus accumbens or striatum and was without marked effect on spontaneous locomotor activity. 3. In the mouse, (+)-HA-966 (30 and 100 mg kg-1) dose-dependently blocked the enhancement of dopamine synthesis induced in the nucleus accumbens by amphetamine, but was without effect on the increase in dopamine synthesis in the striatum. 4. Intracerebroventricular administration of the glycine/NMDA receptor antagonist, 5,7-dichlorokynurenic acid, in the mouse (10 micrograms) also significantly attenuated amphetamine-enhanced DOPA accumulation in the nucleus accumbens, but not in the striatum. 5. The decrease of dopamine synthesis in striatum and nucleus accumbens induced by the dopamine receptor agonist, apomorphine, was unaffected by (+)-HA-966 (100 mg kg-1). 6. (+)-HA-966 (30 mg kg-1) failed to attenuate the hyperactivity induced by the systemic administration of amphetamine in the mouse, but totally prevented the hyperlocomotion following infusion of amphetamine into the rat nucleus accumbens. In contrast, stereotyped behaviour induced by infusion of amphetamine into the rat striatum was not altered following pretreatment with (+)-HA-966 (30 mg kg-1). 7. The results are consistent with a selective facilitatory role of glycine/NMDA receptors on mesolimbic dopaminergic neurones.
1′,5′‐Anhydrohexitol Oligonucleotides: Hybridisation and Strand Displacement with Oligoribonucleotides, Interaction with RNase H and HIV Reverse Transcriptase (1997)

Abstract Hexitol nucleic acids (HNAs) with four natural bases form stable and sequence‐selective duplexes with RNA. This was investigated by T m determinations and gel shift experiments. The CD spectra of an HNA‐RNA duplex show similarities with the CD spectra of the A‐form of dsRNA. Single‐stranded HNAs are able to induce strand displacement in a double‐stranded RNA sequence. An HNA‐RNA duplex is a poor substrate for RNase H, and can inhibit the RNase H‐mediated cleavage of a natural DNA‐RNA substrate. The HNA‐RNA hybrid enhances the activity of HIV reverse transcriptase.
Traumatic brain injury, hemorrhagic shock, and fluid resuscitation: effects on intracranial pressure and brain compliance (1993)

Intracranial hypertension following traumatic brain injury is associated with considerable morbidity and mortality. Hemorrhagic hypovolemia commonly coexists with head injury in this population of patients. Therapy directed at correcting hypovolemic shock includes vigorous volume expansion with crystalloid solutions. It is hypothesized that, following traumatic brain injury, cerebrovascular dysfunction results in rapid loss of brain compliance, resulting in increased sensitivity to cerebrovascular venous pressure. Increased central venous pressure (CVP) occurring with vigorous crystalloid resuscitation may therefore contribute to the loss of brain compliance and the development of intracranial hypertension. The authors tested this hypothesis in miniature swine subjected to traumatic brain injury, hemorrhage, and resuscitation. Elevated CVP following resuscitation from hemorrhage to a high CVP significantly worsened intracranial hypertension in animals with concurrent traumatic brain injury, as compared to animals subjected to traumatic brain injury alone (mean +/- standard error of the mean: 33.0 +/- 2.0 vs. 20.0 +/- 2.0 mm Hg, p < 0.05) or to animals subjected to the combination of traumatic brain injury, hemorrhage, and resuscitation to a low CVP (33.0 +/- 2.0 vs. 24.0 +/- 2.0 mm Hg, p < 0.05). These data support the hypothesis that reduction in brain compliance can occur secondary to elevation of CVP following resuscitation from hemorrhagic shock. This may worsen intracranial hypertension in patients with traumatic brain injury and hemorrhagic shock.
Interpretation of automated perimetry for glaucoma by neural network. (1994)

The authors conclude that a neural network can be taught to be as proficient as a trained reader in interpreting visual fields for glaucoma.
The Antioxidants and Pro-Antioxidants Network: An Overview (2004)

Living beings have evolved over the past two billion years through adaptation, to an increasing atmospheric oxygen concentration, by both taking advantage of oxygen activating function and developing a complex control network. In these regards, potentially damaging species (reactive oxygen, nitrogen and chlorine species) arise as by-products of metabolism and also work as physiological mediators and signalling molecules. Oxidative stress may be an important factor in numerous pathological conditions, i.e. infection if micronutrients are deficient. Levels of these species are controlled by the antioxidant defence system, which is composed by antioxidants and pro-antioxidants. Several components of this system are micronutrients (e.g. vitamins C and E), are dependent upon dietary micronutrients (e.g. CuZn and Mn superoxide dismutase) or are produced by specific endogenous pathways. The antioxidant defences act, to control levels of these species, as a coordinated system where deficiencies in one component may affect the efficiency of the others. In this network some of the components act as direct antioxidants whereas others act indirectly (pro-antioxidants) either by modulation of direct agents or by regulation of the biosynthesis of antioxidant proteins. Thus, entities usually not considered as antioxidants, also act efficiently counteracting damaging effects of oxidative species. In this contest, the design of new molecules that take into account synergistic interactions among different antioxidants, could be useful both to address mechanistic studies and to develop possible therapeutic agents. In this review the principal categories of antioxidants and pro-antioxidants that goes from vitamins through phyto-derivatives to minerals, are critically reviewed, with particular emphasis on structure-function considerations, together with the perspective opened, in the design of possible therapeutic agents, by the antioxidants interplay.
Dietary restriction and 2-deoxyglucose administration improve behavioral outcome and reduce degeneration of dopaminergic neurons in models of Parkinson's disease (1999)

Parkinson's disease (PD) is an age-related disorder characterized by progressive degeneration of dopaminergic neurons in the substantia nigra (SN) and corresponding motor deficits. Oxidative stress and mitochondrial dysfunction are implicated in the neurodegenerative process in PD. Although dietary restriction (DR) extends lifespan and reduces levels of cellular oxidative stress in several different organ systems, the impact of DR on age-related neurodegenerative disorders is unknown. We report that DR in adult mice results in resistance of dopaminergic neurons in the SN to the toxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). MPTP-induced loss of dopaminergic neurons and deficits in motor function were ameliorated in DR rats. To mimic the beneficial effect of DR on dopaminergic neurons, we administered 2-deoxy-D-glucose (2-DG; a nonmetabolizable analogue of glucose) to mice fed ad libitum. Mice receiving 2-DG exhibited reduced damage to dopaminergic neurons in the SN and improved behavioral outcome following MPTP treatment. The 2-DG treatment suppressed oxidative stress, preserved mitochondrial function, and attenuated cell death in cultured dopaminergic cells exposed to the complex I inhibitor rotenone or Fe2+. 2-DG and DR induced expression of the stress proteins heat-shock protein 70 and glucose-regulated protein 78 in dopaminergic cells, suggesting involvement of these cytoprotective proteins in the neuroprotective actions of 2-DG and DR. The striking beneficial effects of DR and 2-DG in models of PD, when considered in light of recent epidemiological data, suggest that DR may prove beneficial in reducing the incidence of PD in humans.
Neuropeptide Y Stimulates Feeding but Inhibits Sexual Behavior in Rats* (1985)

The effects of neuropeptide Y (NPY), a tyrosine-rich peptide found in the rat brain, on feeding and sexual behavior were studied in male and female rats. Intraventricular (ivt) injections of NPY during the final hours of the light period induced feeding in a dose-related manner. While the lowest dose tested (0.02 nM) was without effect, higher doses (0.12, 0.47, 2.3 nM) uniformly elicited feeding with a latency of about 15 min in male rats. With the most effective dose, 0.47 nM, the increased food intake was due to an increased local eating rate. In contrast, the pattern of feeding behavior after a related peptide, rat pancreatic polypeptide (rPP), was quite different and less impressive. During the first hour, only one ivt dose of rPP (0.45 nM) evoked an increase in food intake, due to an increased time spent eating. Further, the effects of NPY on food intake were greater during the nocturnal period. Interestingly, increased food intake in nocturnal tests (4 h) was due solely to augmented intake during the first 60 min after ivt administration. In mating tests, initiated 2 h after the onset of darkness and 10 min after ivt administration of peptide, all but the lowest dose of NPY (0.01 nM) drastically suppressed ejaculatory behavior. Most rats treated with higher doses of NPY (0.02, 0.12, or 0.47 nM) mounted and intromitted only a few times before the cessation of sexual activity, and elongated latencies to the initial mount and intromission were observed. In contrast to the dramatic NPY-induced suppression of ejaculatory behavior, rPP (0.11 and 0.45 nM) was without effect on copulatory behavior. To substantiate further that the impairment of sexual behavior seen in NPY-treated rats was not due to an attenuated sexual ability, an additional experiment was performed. Penile reflexes, including erection, were monitored 10 min after ivt injection of NPY (0.12 nM), rPP (0.11 nM), or saline. No effect of NPY or rPP was observed on the proportion of rats showing erection or latency to initial erection, or in the number of erections per test. In fact, a slight facilitation of penile dorsiflexion responses was seen after NPY. These findings suggest that NPY selectively depresses sexual motivation in the male rat. In ovariectomized female rats responding to estrogen plus progesterone with a good level of sexual receptivity (lordosis quotient greater than 70), ivt saline and 0.01 nM NPY were without effect on sexual behavior.(ABSTRACT TRUNCATED AT 400 WORDS)
Design and Synthesis of a Self-Assembled Photochemical Dyad Based on Selective Imidazole Recognition (2002)

The unique recognition properties of phenanthroline-strapped zinc porphyrin 1, which displays extremely high affinity for N-unsubstituted imidazoles, has been used as the driving force for the assembly of a photochemical dyad involving a zinc(II) porphyrin as energy donor and a free base porphyrin as energy acceptor. The synthesis of the imidazole-substituted porphyrin is described together with the assembly of the dyad. (1)H NMR titrations confirm the formation of a 1/1 complex between 1 and 6, as well as insertion of the imidazole of the acceptor within the phenanthroline strap of the donor. Preliminary fluorescence quenching measurements show that efficient energy transfer occurs between the self-assembled components.
Voluntary Smiling Changes Regional Brain Activity (1993)

We used measures of regional brain electrical activity to show that not all smiles are the same. Only one form of smiling produced the physiological pattern associated with enjoyment. Our finding helps to explain why investigators who treated all smiles as the same found smiles to be ubiquitous, occurring when people are unhappy as well as happy. Also, our finding that voluntarily making two different kinds of smiles generated the same two patterns of regional brain activity as was found when these smiles occur involuntarily suggests that it is possible to generate deliberately some of the physiological change which occurs during spontaneous positive affect.
Alkaline citrate reduces stone recurrence and regrowth after shockwave lithotripsy and percutaneous nephrolithotomy (2011)

Sodium-potassium citrate provides positive effects on stone-forming activities in calcium stone patients suffering from urolithiasis following treatment with ESWL and PCNL procedures at the 12-month follow-up.
István Fenyő (1917–1987) in memoriam (1992)

Professor István (Stefan, Stephen, Étienne) Fenyö, a frequent and active participant of these General Inequalities meetings, passed away since our last conference, less than five months past his 70th birthday after a painful but mercifully short illness.
On the application of supported bilayers as receptive layers for biosensors with electrical detection (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOn the application of supported bilayers as receptive layers for biosensors with electrical detectionM. Stelzle, G. Weissmueller, and E. SackmannCite this: J. Phys. Chem. 1993, 97, 12, 2974–2981Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/j100114a025https://doi.org/10.1021/j100114a025research-articleACS PublicationsRequest reuse permissionsArticle Views715Altmetric-Citations247LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Tracking Microchimeric DNA in Plasma to Diagnose and Manage Organ Transplant Rejection (2006)

One of the most promising areas of transplantation research is the recent discovery of biomarkers for rejection that are detectable in blood and urine. Biopsy-confirmed rejection, the current gold standard for diagnosis of allograft rejection, is invasive and subject to sampling errors. For example, diagnosis of episodes of rejection of pancreas allografts, which are frequent and can destroy the allograft, depends on conventional percutaneous biopsies that have a diagnostic sensitivity of only 79%–88% (1)(2) and are associated with a 2%–7% risk of serious complications (3). Development of noninvasive assays that detect molecular biomarkers for rejection could revolutionize management of transplant recipients by ( a ) detecting a prerejection profile that will allow therapeutic interventions before rejection causes graft dysfunction; ( b ) improving the sensitivity and specificity of rejection diagnosis; ( c ) developing new classification systems for rejection that will improve prognosis; and ( d ) providing information for designing individualized immunosuppressive regimens that could prevent rejection while minimizing drug toxicity.

There have been several exciting reports of potential biomarkers for allograft rejection, with the most significant progress occurring in the area of renal transplantation. This work began by studying concentrations of particular mRNAs or proteins that were associated with immune activation or tissue stress (4). These studies have revealed several gene products that have altered expression in blood, urine, and/or biopsy tissue during rejection episodes. In the most recent contribution in this rapidly evolving field, Muthukumar et al. (5) demonstrated that urine concentrations of FOXP3 mRNA, a member of the forkhead family of cell differentiation genes and a lineage-specific transcript for graft-protecting regulatory T cells, can predict reversal of acute renal allograft rejection with 90% sensitivity and 73% specificity. Although measurement of the products of individual genes such as FOXP3 will probably not supplant conventional biopsies for diagnosis of rejection, development of panels of …
EFFECT OF PLANTING DENSITY ON THE YIELD OF COCONUT (2010)

An analyses is presented of the yield components of coconut established underdensities varying from 128 palms/ha to 239 palms/ha from a spacing trial conductedby the Coconut Research Institute. Analysis of yield data during 1976-1980 from 24palms from 4 replicates analysed for each density treatment revealed significantdifferences in nuts/palm and copra/palm for the densities tested. The nuts/palmdecreased with increasing density but the yield/ha maximised at a density of 171palms/ha and decreased beyond 171. Significant differences were absent in yield ofcopra/nut for the densities tested. The differences in copra/palm were due to theeffect of nut number/palm. At a density of 179 palms/ha, copra/ha maximised to2260 kg and decreased beyond 179. The results of the present analysis indicate thata density ranging from 171-179 palms per hectare is the optimum for plantingcoconut in dry-intermediate zone where the study was conducted.
Nueva época, otra Historia (1992)

[ES] Nueva época, otra Historia. Artículo de Ángel Rodríguez Sánchez y Baltasar Cuart Moner.
Evidence for a role of osteopontin in macrophage infiltration in response to pathological stimuli in vivo. (1998)

Previous studies have suggested that osteopontin, an arginine-glycine-aspartate-containing secreted protein, might be important in macrophage accumulation during interstitial nephritis and wound healing. The current study investigated the role of osteopontin in intradermal macrophage infiltration using immunohistochemistry and neutralizing antibodies. Purified osteopontin induced macrophage accumulation after injection in rat dermis and facilitated adhesion and migration of cultured macrophage-like cells in vitro. Intradermal injection of N-formyl-met-leu-phe (FMLP), a potent macrophage chemotactic peptide, induced a macrophage-rich infiltrate at the site of injection. Most of these macrophages expressed high levels of osteopontin as shown by immunochemical analysis. To determine whether osteopontin expressed by macrophages was required for their infiltration, we treated rats with either anti-osteopontin neutralizing antibody (OP199) or nonimmune antibody. We found that FMLP-induced macrophage accumulation was largely inhibited (>60%) by anti-osteopontin antibody treatment compared with rats receiving nonimmune antibody. These data support the hypothesis that osteopontin may be a critical mediator of inflammation in specific disease and injury states, potentially by promoting macrophage adhesion and migration.
Review : V region gene analysis of human IgM hybridoma monoclonal anti-Sm antibodies (1997)

Anti-Sm antibodies although highly specific for systemic lupus erythematosus can only be found in 10-25% of lupus patients and lupus-prone MRL/lpr mice. Molecular studies of these autoantibodies from mice have suggested that the anti-Sm response is Ag driven, its expression is controlled by stochastic events and may originate from the same B cell precursors as anti-DNA antibodies. However, relatively little information regarding the molecular characteristics of anti-Sm antibodies in man has been reported. We studied the V region genes of three IgM hybridoma monoclonal antibodies (BUD 45.12.8, BUD 114.4.11 and BUD 94.91.8) which were selected for Sm reactivity and derived from B cells of a healthy child. Two of these antibodies BUD 45.12.8 and BUD 114.4.11 also reacted with ssDNA, while the third (BUD 94.91.8) did not. Each of these anti-Sm/ RNP antibodies was encoded by different and predominantly unmutated Ig heavy chain germline genes (BUD 45.12.8 by V H 3-23, DXP4 and J H 4b; BUD 94.91.8 by V H 3-33, D21-9 and J H 6b; BUD 114.4.11 by V H 1-2, DK1 or DM1 or unknown D and JH4b) and light chain genes (BUD 45.12.8 by Humkv325 and Jκ2; BUD 94.91.8 by hsigg11150 (λIIIb) and Jλ2/3; BUD 114.4.11 by Humk18 and Jκ3). Many of these genes are also used by antibodies with other specificities including DNA. The two anti-Sm antibodies which also bound ssDNA shared an overall V region net positive charge, while the third antibody without ssDNA reactivity carried a negative V region net charge. These findings demonstrate that (1) normal individuals have the genetic potential to generate autoantibodies to Sm/RNP; (2) acquisition of Sm/RNP binding is not dependent on somatic mutations and (3) some human B cell clones exhibit specificity for Sm and ssDNA.