gebhart/patrepeval huggingface dataset:
Electrostatic N-95 Respirator Filter Media Efficiency Degradation Resulting from Intermittent Sodium Chloride Aerosol Exposure (2000)

The effects of intermittently loading small masses of sodium chloride aerosol on the filtration efficiency of N-95 filtering facepiece respirators was investigated. The National Institute for Occupational Safety and Health (NIOSH) certifies that N-95 respirators must provide at least 95 percent filtration efficiency against a sodium chloride aerosol challenge as per the respirator certification (42 CFR 84) test criteria. N-95 respirators are specified for protection against solid and water-based particulates (i.e., non-oil aerosols). New N-95 respirators from three different manufacturers were loaded with 5 +/- 1 mg of sodium chloride aerosol one day a week, over a period of weeks. Aerosol loading and penetration measurements were performed using the TSI 8130 Filter Tester. Respirators were stored uncovered on an office desktop outside the laboratory. To investigate environmental and temporal effects of filters being stored without sodium chloride exposure, control respirators were stored on the desk for various lengths of time before being initiated into weekly testing. For all manufacturers' respirators, the controls showed similar initial penetrations on their day of initiation (day zero) to those of the study samples on day zero. As the controls were tested weekly, they showed similar degradation rates to those of the study samples. Results show that some of the manufacturers' models had penetrations of greater than 5 percent when intermittently exposed to sodium chloride aerosol. It is concluded that intermittent, low-level sodium chloride aerosol loading of N-95 respirators has a degrading effect on filter efficiency. This reduction in filter efficiency was not accompanied by a significant increase in breathing resistance that would signal the user that the filter needs to be replaced. Furthermore, it was noted that the effect of room storage time prior to initial exposure was much less significant.
Postgrowth wavelength tuning of optoelectronic devices by ion implantation induced quantum well intermixing (2005)

As one of the postgrowth band gap engineering technique, proton implantation induced quantum well intermixing has been used to tune the wavelength of two different optoelectronic devices, the quantum well laser and quantum well infrared photodetector. Large wavelength shifts have been obtained for both devices after implantation and rapid thermal annealing, without significant degradation of performance.
The impact of counselling on HIV-infected women in Zimbabwe (1998)

The objective of this study was to examine the impact of counselling provided for HIV-infected women in Zimbabwe. Qualitative research was used for data collection. In total, 44 women were heard; most were members of an HIV support group. Doctors and nurses play an important role in the first counselling session, because tests to diagnose HIV-infection are done in the hospital. Interviewed women mention slow disclosure of status as the most comforting way to hear the news. The HIV-infected women experience strong emotions directly after diagnosis. Counselling at this moment is of major importance to reduce fear and can prevent suicide. Women should be prevented from discovering their status on their own. Counselling given once is found not to be effective. First, if only one counselling session is given, the women may not hear or remember all that is said. Second, in case of depression, access to counselling is important and it appears that periods of depression return frequently. Support groups play an important role in providing this continuous counselling. Another advantage of the counselling provided by HIV-positive women of a support group is that the counsellors function as examples.
Suppressors of Superoxide Dismutase (SOD1) Deficiency in Saccharomyces cerevisiae (1998)

Yeast deficient in the cytosolic copper/zinc superoxide dismutase (SOD1) exhibit metabolic defects indicative of oxidative damage even under non-stress conditions. To help identify the endogenous sources of this oxidative damage, we isolated mutant strains of S. cerevisiae that suppressed metabolic defects associated with loss of SOD1. Six complementation groups were isolated and three of the corresponding genes have been identified. One sod1Delta suppressor represents SSQ1 which encodes a hsp70-type molecular chaperone found in the mitochondria. A second sod1Delta suppressor gene, designated JAC1, represents a new member of the 20-kDa J-protein family of co-chaperones. Jac1p contains a mitochondrial targeting consensus sequence and may serve as the partner for Ssq1p. Homologues of Ssq1p and Jac1p are found in bacteria in close association with genes proposed to be involved in iron-sulfur protein biosynthesis. The third suppressor gene identified was NFS1. Nfs1p is homologous to cysteine desulfurase enzymes that function in iron-sulfur cluster assembly and is also predicted to be mitochondrial. Each of the suppressor mutants identified exhibited diminished rates of respiratory oxygen consumption and was found to have reduced mitochondrial aconitase and succinate dehydrogenase activities. Taken together these results suggest a role for Ssq1p, Jac1p, and Nfs1p in assembly/maturation of mitochondrial iron-sulfur proteins and that one or more of the target Fe/S proteins contribute to oxidative damage in cells lacking copper/zinc SOD.
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (1989)

MyoD is a master regulatory gene for myogenesis. Under the control of a retroviral long terminal repeat, MyoD was expressed in a variety of differentiated cell types by using either a DNA transfection vector or a retrovirus. Expression of muscle-specific proteins was observed in chicken, human, and rat primary fibroblasts and in differentiated melanoma, neuroblastoma, liver, and adipocyte lines. The ability of MyoD to activate muscle genes in a variety of differentiated cell lines suggests that no additional tissue-specific factors other than MyoD are needed to activate the downstream program for terminal muscle differentiation or that, if such factors exist, they are themselves activated by MyoD expression.
Gene-Specific Effects of Antisense Phosphorodiamidate Morpholino Oligomer-Peptide Conjugates onEscherichia coliandSalmonella entericaSerovar Typhimurium in Pure Culture and inTissue Culture (2006)

The objective was to improve efficacy of antisense phosphorodiamidate morpholino oligomers (PMOs) by improving their uptake into bacterial cells. Four different bacterium-permeating peptides, RFFRFFRFFXB, RTRTRFLRRTXB, RXXRXXRXXB, and KFFKFFKFFKXB (X is 6-aminohexanoic acid and B is beta-alanine), were separately coupled to two different PMOs that are complementary to regions near the start codons of a luciferase reporter gene (luc) and a gene required for viability (acpP). Luc peptide-PMOs targeted to luc inhibited luciferase activity 23 to 80% in growing cultures of Escherichia coli. In cell-free translation reactions, Luc RTRTRFLRRTXB-PMO inhibited luciferase synthesis significantly more than the other Luc peptide-PMOs or the Luc PMO not coupled to peptide. AcpP peptide-PMOs targeted to acpP inhibited growth of E. coli or Salmonella enterica serovar Typhimurium to various extents, depending on the strain. The concentrations of AcpP RFFRFFRFFXB-PMO, AcpP RTRTRFLRRTXB-PMO, AcpP KFFKFFKFFKXB-PMO, and ampicillin that reduced CFU/ml by 50% after 8 h of growth (50% inhibitory concentration [IC(50)]) were 3.6, 10.8, 9.5, and 7.5 microM, respectively, in E. coli W3110. Sequence-specific effects of AcpP peptide-PMOs were shown by rescuing growth of a merodiploid strain that expressed acpP with silent mutations in the region targeted by AcpP peptide-PMO. In Caco-2 cultures infected with enteropathogenic E. coli (EPEC), 10 microM AcpP RTRTRFLRRTXB-PMO or AcpP RFFRFFRFFXB-PMO essentially cleared the infection. The IC(50) of either AcpP RTRTRFLRRTXB-PMO or AcpP RFFRFFRFFXB-PMO in EPEC-infected Caco-2 culture was 3 microM. In summary, RFFRFFRFFXB, RTRTRFLRRTXB, or KFFKFFKFFXB, when covalently bonded to PMO, significantly increased inhibition of expression of targeted genes compared to PMOs without attached peptide.
Detection of high mass molecular ions by laser desorption time-of-flight mass spectrometry. (1988)

A laser desorption time-of-flight (TOF) mass spectrometer has been developed in order to analyze non-volatile, thermally labile and high mass organic compounds, in which the “gradient electric field type ion reflector” was used for time focusing. Higher detection sensitivity for high mass ions was achieved by adding a blind type ion-electron converter to the micro-channel plate (MCP) ion detector. Two TOF spectrum measurement systems were constructed, with which two measurement methods called as “AD method” and “TDC method” were able to be selected to obtain the spectrum sensitively and accurately in a short time.For sample preparation, “metal fine powder and glycerol matrix” method was confirmed to be very effective to increase the yield of high mass molecular ions.This mass spectrometer was successfully applied to the detection of quasi-molecular ions from many high mass organic compounds, such as Cytochrome-C (mol. wt. 12,384), Bovine Trypsin (mol. wt. ~24,000) and Carboxypeptidase-A (mol. wt. 34,472).
Interruption of the Ras/MEK/ERK signaling cascade enhances Chk1 inhibitor–induced DNA damage in vitro and in vivo in human multiple myeloma cells (2008)

The role of the Ras/MEK/ERK pathway was examined in relation to DNA damage in human multiple myeloma (MM) cells exposed to Chk1 inhibitors in vitro and in vivo. Exposure of various MM cells to marginally toxic concentrations of the Chk1 inhibitors UCN-01 or Chk1i modestly induced DNA damage, accompanied by Ras and ERK1/2 activation. Interruption of these events by pharmacologic (eg, the farnesyltransferase inhibitor R115777 or the MEK1/2 inhibitor PD184352) or genetic (eg, transfection with dominant-negative Ras or MEK1 shRNA) means induced pronounced DNA damage, reflected by increased gammaH2A.X expression/foci formation and by comet assay. Increased DNA damage preceded extensive apoptosis. Notably, similar phenomena were observed in primary CD138(+) MM cells. Enforced MEK1/2 activation by B-Raf transfection prevented R115777 but not PD184352 from inactivating ERK1/2 and promoting Chk1 inhibitor-induced gammaH2A.X expression. Finally, coadministration of R115777 diminished UCN-01-mediated ERK1/2 activation and markedly potentiated gammaH2A.X expression in a MM xenograft model, associated with a striking increase in tumor cell apoptosis and growth suppression. Such findings suggest that Ras/MEK/ERK activation opposes whereas its inhibition dramatically promotes Chk1 antagonist-mediated DNA damage. Together, these findings identify a novel mechanism by which agents targeting the Ras/MEK/ERK pathway potentiate Chk1 inhibitor lethality in MM.
Use of Surfactant to Reduce CO2 Mobility in Oil Displacement (1980)

Abstract This paper presents results from a study designed to improve effectiveness of CO2 flooding by reducing CO2 mobility. In the course of reaching this objective we (1) screened surfactants for their ability to generate an effective and stable emulsion with CO2 under reservoir conditions, (2) determined the concentration range over which surfactants were effective, (3) examined chemical stability of the surfactants at reservoir conditions, (4) determined the extent to which emulsifying action alters gas and liquid mobilities in carbonate and sandstone cores, (5) determined that surfactant can enhance the production of residual oil from watered-out carbonate cores by CO2, and (6) showed that the permeability reduction caused by surfactant can be dissipated. At reservoir conditions required for miscible displacement, carbon dioxide exists in its critical state as a very dense fluid whose viscosity is about one-eighth that of crude oil. Generally, this unfavorable viscosity and mobility ratio produces inefficient oil displacement. This study shows that surfactant reduces CO2 mobility and should improve oil displacement by CO2, presumably by reducing flow through the most permeable zones, thus increasing areal and vertical sweep efficiencies. All three classes of surfactants (anionic, cationic, and nonionic) were found to be stable under conditions encountered during a CO2 flood in limestone formation; however, only a few surfactants had proper adsorption and emulsifying properties. Surfactant generated foams or emulsions with CO2 at reservoir conditions (1,000 to 3,000 psi and 135°F) dramatically reduced CO2 flow through sandstone and carbonate cores. Surfactant reduced the amount of CO2 used to recover a given volume of oil, especially from watered-out cores. The mechanism of tertiary oil production from linear cores appears to be limited to CO2 extraction. Approximately the same oil recovery was obtained either by continuous CO2 injection after a surfactant slug or by alternate slugs of CO2 and surfactant solution. It was found that oil recovery efficiency increased when surfactant was used with CO2 and that efficiency increased with flooding pressure. One anionic surfactant was found to be superior for this purpose. This surfactant emulsified CO2 well, was least adsorbed on carbonate rocks, and greatly reduced CO2 mobility in linear cores at concentrations of 0.1 to 1%. The study indicates that effectiveness of CO2 miscible flooding can be increased by alternate injection of CO2 and aqueous surfactant slugs into the reservoir.
Continuously moving table data acquisition method for long FOV contrast‐enhanced MRA and whole‐body MRI (2002)

A method is presented in which an extended longitudinal field of view (FOV), as required for whole-body MRI or MRA peripheral runoff studies, is acquired in one seamless image. Previous methods typically either acquired 3D data at multiple static "stations" which covered the extended FOV or as a series of 2D axial sections. The method presented here maintains the benefits of 3D acquisition while removing the discrete nature of the multistation method by continuous acquisition of MR data as the patient table moves through the desired FOV. Although the technique acquires data only from a homogeneous central volume of the magnet at any point in time, by spatially registering all data it is possible to extend the FOV well beyond this volume. The method is demonstrated experimentally with phantoms, in vivo angiographic animal studies, and in vivo human studies.
Sustained activation of vascular cells and leukocytes in the rabbit aorta after balloon injury. (1993)

Local inflammatory activation of endothelial cells, SMCs, and leukocytes occurs in a predictable sequence and persists up to 30 days after balloon injury to the rabbit aorta. Our findings suggest that ongoing local signals persisting after the original balloon injury may contribute to later phases of intimal thickening.
Type I Gaucher Disease, a Glycosphingolipid Storage Disorder, Is Associated with Insulin Resistance (2007)

Gaucher disease, a lysosomal glycosphingolipid storage disorder, is associated with (peripheral) insulin resistance, possibly through the influence of glycosphingolipids on insulin receptor functioning.
The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA (2012)

The RNA binding protein heterogeneous nuclear ribonucleoprotein (hnRNP) F is involved in telomeres maintenance and pre-mRNA processing, such as alternative splicing and polyadenylation. It specifically recognizes RNA containing three consecutive guanines (G-tracts) that have the potential to assemble into G-quadruplexes. We have proposed recently that hnRNP F could regulate alternative splicing by remodeling RNA structures, such as G-quadruplexes. However, the exact mechanism of hnRNP F binding to such RNA sequences remains unknown. Here, we have studied the binding of the third RNA binding domain of hnRNP F [quasi-RNA recognition motif 3 (qRRM3)] to G-tract RNA using isothermal titration calorimetry, circular dichroism and nuclear magnetic resonance spectroscopy. Our results show that qRRM3 binds specifically exclusively to single-stranded G-tracts (ssRNA), in contrast to previous reports stating that the G-quadruplex was recognized as well. Furthermore, we demonstrate that the pre-existent ssRNA/G-quadruplex equilibrium slows down the formation of the protein-ssRNA complex. Based on in vitro transcription assays, we show that the rate of the protein-RNA complex formation is faster than that of the G-quadruplex. We propose a model according to which hnRNP F could bind RNA co-transcriptionally and prevents G-quadruplex formation.
IRX-2, a Novel In Vivo Immunotherapeutic, Induces Maturation and Activation of Human Dendritic Cells In Vitro (2007)

IRX-2 is a uniform, well-defined set of natural cytokines currently in Phase II clinical trials for squamous cell carcinoma of the head and neck (HNSCC). In preliminary clinical studies of HNSCC patients, IRX-2 therapy has shown promising results, increasing overall survival of patients from 32% to 61% at 48 months. Although it is known that specific cytokines in IRX-2 enhance T cell activity [e.g., interleukin-2 (IL-2), interferon-gamma, IL-1beta], we chose to investigate the influence of IRX-2 on monocyte-derived dendritic cells (Mo-DCs) isolated from human peripheral blood in an effort to further understand the clinical findings. We show here that IRX-2 treatment of human monocyte-derived DC resulted in morphologic, phenotypic, and functional changes consistent with the development of mature activated DC. Specifically, IRX-2-treated DC increased expression of CD83 and CCR7, markers for DC maturation and migration, respectively, and increased the expression of HLA-DR, CD54, and the costimulatory molecules CD86 and CD40, which are critical mediators of T cell activation. Functional changes in DC induced by IRX-2 included a reduced endocytic capacity, increased ability to stimulate T cells and increased IL-12 cytokine production. These results provide a plausible mechanistic explanation for the in vivo clinical activity of IRX-2 and an additional rationale for the use of IRX-2-based immunotherapy in patients.
Multiwavelength 10-GHz picosecond pulse generation from a single-stripe semiconductor diode laser (1997)

A single-stripe GaAs-AlGaAs semiconductor optical amplifier (SOA) has been used to generate four tunable wavelength-division multiplexed (WDM) channels simultaneously, each transmitting 12-ps pulses at 2.5 GHz, for an aggregate pulse rate of 10 GHz. Wavelength tuning over 18 nm has been demonstrated with channel spacing ranging from 0.8 to 2.1 nm. A potential spectral correlation across the multiwavelength spectrum has been studied in both experiment and simulation methods. These results show the potential for utilizing single stripe laser diodes as multiwavelength sources in WDM-TDM network.
Improved sensitivity of biological sequence database searches (1990)

We have increased the sensitivity of DNA and protein sequence database searches by allowing similar but non-identical amino acids or nucleotides to match. In addition, one can match k-tuples or words instead of matching individual residues in order to speed the search. A matching matrix species which k-tuples match each other. The matching matrix can be calculated from a similarity matrix of amino acids and a threshold of similarity required for matching. This permits amino acid similarity matrices or replacement matrices (PAM matrices) to be used in the first step of a sequence comparison rather than in a secondary scoring phase. The concept of matching non-identical k-tuples also increases the power of DNA database searches. For example, a matrix that specifies that any 3-tuple in a DNA sequence can match any other 3-tuple encoding the same amino acid permits a DNA database search using a DNA query sequence for regions that would encode a similar amino acid sequence.
Resistance to glucocorticoid-induced apoptosis in human T-cell acute lymphoblastic leukemia CEM-C1 cells is due to insufficient glucocorticoid receptor expression. (1996)

The ability of glucocorticoids (GCs) to induce death in lymphoid-origin cells is the basis for their frequent use in the therapy of various human hematological malignancies. However, the occurrence of primary or secondary GC resistance limits their clinical usefulness. Prior investigations into the mechanism of GC resistance in established human leukemic cell lines revealed loss-of-function mutations in the GC receptor (GR) gene. In this study, we analyzed the GC-resistant human acute T-cell leukemia line CEM-C1, which has been reported to express biochemically functional GR and, thus, was thought to owe its GC resistance to signal transduction changes distal from the GR. Radioligand binding assays revealed a 2-3-fold lower expression of GR in CEM-C1 than in the GC-sensitive sister cell line CEM-C7H2. Analysis of transcriptional activity using mouse mammary tumor virus-long terminal repeat-controlled chloramphenicol acetyltransferase expression in transient transfection assays confirmed the expression of functional GR in CEM-C1 but at levels lower than those in CEM-C7H2 cells. Upon molecular analyses of the GR gene and its transcripts, we found that CEM-C1 cells were heterozygous for the ligand binding domain L753F point mutation in exon 9, which is also present in GC-sensitive CEM-C7H2. No mutations, however, were found on the second GR allele of CEM-C1. To test the possibility that resistance in CEM-C1 cells might be caused by insufficient expression of GR, we established several cell lines stably transfected with rat GR expression vectors. These cell lines differed in exogenous GR expression as determined by Northern blotting and radioligand binding assays. The GR expression level in individual lines correlated well with their sensitivity to GC-induced apoptosis. Thus, GC resistance of CEM-C1 cells might be due to subthreshold expression of functional GR rather than defects in signal transduction pathways distal from the GR. Since several clinical investigations showed a correlation between reduced GR expression and poor response to GC-containing treatment, the CEM-C1 line may represent a valid model for GC resistance in human acute T-cell leukemia.
Epidemiological and clinical study of viral respiratory tract infections in children from Italy (2009)

Impact of recently discovered viruses on epidemiology of acute respiratory tract infections (ARTI) is still unclear. We studied the impact of recently discovered human metapneumovirus (hMPV), human bocavirus (HBoV), and new coronaviruses (HCoV-NL63 and HKU1) on the global epidemiology of ARTI. From October 2006 to April 2007, 237 pediatric patients affected by ARTI were enrolled in our study. Specimens were tested for respiratory viruses by polymerase chain reaction. One hundred twenty-four out of 237 samples (52.3%) were positive for one or more viruses. Picornaviruses were the most prevalent viruses (n = 61, 43.6%), followed by respiratory syncytial virus (n = 34, 24.3%) and Adenovirus (n = 25, 17.9%); hMPV (n = 9, 6.4%) was the fourth most common virus detected. HBoV and HCoV showed a low prevalence (respectively 2.9% and 2.1%). RSV was the prevalent agent of LRTI (38%). Viruses were identified in more than 50% of the studied ARTI, providing useful information on clinical features and epidemiology of specific agents affecting children in cold months. Although routine surveillance of respiratory viruses does not seem cost-effective, continuous monitoring of ARTI etiology could be a useful tool for planning resources for the development of new vaccines and antiviral agents.