gebhart/patrepeval huggingface dataset:
Detection of antibodies cross-reactive with type C RNA tumor viral p30 protein in human sera and exudate fluids. (1980)

Human sera and human exudate fluids were surveyed for the presence of antibodies cross-reacting with a type C viral protein with a molecular weight of 30,000 (p30). Antibodies cross-reactive with type C viral p30 were concentrated by means of affinity chromatography using Sepharose beads to which disrupted Simian sarcoma virus or Rauscher murine leukemia virus p30 had been coupled. Eluates obtained from the affinity beads were tested for activity against Rauscher murine leukemia virus p30, Simian sarcoma virus p30, and endogenous feline virus RD-114 p30 by radioimmunoprecipitation. Of 57 eluates tested, 29 showed activity against type C viral p30. A number of eluates were also tested for anti-p30 activity by a visual microcytotoxicity assay. Of 11 eluates positive in the radioimmunoprecipitation assay, five were also positive in the microcytotoxicity assay.
Human endothelial cells synthesize and express an Arg-Gly-Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor. (1987)

Human umbilical vein endothelial cells express a heterodimeric adhesion receptor complex consisting of noncovalently associated alpha and beta subunits that under reducing conditions have molecular masses of 135 kDa and 115 kDa, respectively. This complex can be isolated in pure form from an affinity matrix consisting of an Arg-Gly-Asp-containing heptapeptide and is specifically immunoprecipitated with monoclonal antibodies (mAbs) directed against the vitronectin receptor of human melanoma cells. These data suggest that this complex is one member of a large family of cell adhesion receptors. One of the mAbs, LM609, inhibits the attachment of human endothelial cells to fibrinogen, von Willebrand factor, and vitronectin yet has no effect on the attachment of these cells to fibronectin, collagen, or laminin. In addition, mAb LM609 inhibits attachment of endothelial cells to an immobilized synthetic peptide containing the Arg-Gly-Asp sequence. This adhesion receptor appears structurally similar to the IIb/IIIa glycoprotein complex expressed on platelets yet is antigenically distinct, since mAb LM609 fails to recognize IIb/IIIa glycoproteins. This receptor organizes in clusters on endothelial cells during their attachment to von Willebrand factor, vitronectin, or the Arg-Gly-Asp-containing heptapeptide. The data presented in this report suggest that Arg-Gly-Asp recognition may play a significant role in biological events associated with vascular proliferation.
Nestin expression in normal adrenal gland and adrenocortical tumors. (2005)

Human adrenocortical cells have been shown to express cytokeratins and vimentin. Nestin is an intermediate filament protein that is mainly expressed in the developing nervous system and that has been recently reported in rat adrenal gland as well. Using immunohistochemical and biochemical approaches, the present study demonstrates that nestin is constantly expressed in situ in the cortex of normal human adrenal glands. Nestin expressing cells were prevalently located in the zona reticularis but some positive cells could be spotted in the zona fasciculata as well. Moreover, patches of nestin-positive cells have been constantly detected on sections of cortical adenomas. In contrast, adrenal carcinomas displayed a variable number of nestin-immunoreactive cells that in some cases were virtually absent. Samples of renal clear cell carcinoma metastasis in the adrenals were also examined which did not show nestin-immunoreactivity. We propose that a positive nestin-immunoreaction could be useful in differential diagnosis of clear cell tumors in adrenal glands.
Cloning of a DNA sequence encoding a major fragment of the 47 kilodalton stress protein homologue ofCandida albicans (1989)

Part of the gene coding for the immunodominant 47 kDa antigen of the human fungal pathogen Candida albicans was cloned and sequenced. The predicted amino acid sequence was homologous to stress proteins, the most extensive similarity being with the heat shock protein hsp 90 of Saccharomyces cerevisiae. The 47 kDa antigen has diagnostic and therapeutic potential and is the first candidal antigen to be cloned and sequenced.
Superconducting Cyclotrons (1975)

Superconducting cyclotrons are particularly appropriate for acceleration of heavy ions; this paper reviews design features of a superconducting cyclotron with energy 440 (Q2/A) MeV. A strong magnetic field (4.6 tesla average) leads to small physical size (extraction radius 6 5 cm) and low construction costs. Operating costs are also low. The design is based on established technology (from present cyclotrons and from large bubble chambers). Two laboratories (in Chalk River, Canada and in East Lansing, Michigan) are proceeding with construction of full-scale prototype components for such cyclotrons.
Next-Generation Beneficial Microbes: The Case of Akkermansia muciniphila (2017)

Metabolic disorders associated with obesity and cardiometabolic disorders are worldwide epidemic. Among the different environmental factors, the gut microbiota is now considered as a key player interfering with energy metabolism and host susceptibility to several non-communicable diseases. Among the next-generation beneficial microbes that have been identified, <i>Akkermansia muciniphila</i> is a promising candidate. Indeed, <i>A. muciniphila</i> is inversely associated with obesity, diabetes, cardiometabolic diseases and low-grade inflammation. Besides the numerous correlations observed, a large body of evidence has demonstrated the causal beneficial impact of this bacterium in a variety of preclinical models. Translating these exciting observations to human would be the next logic step and it now appears that several obstacles that would prevent the use of <i>A. muciniphila</i> administration in humans have been overcome. Moreover, several lines of evidence indicate that pasteurization of <i>A. muciniphila</i> not only increases its stability but more importantly increases its efficacy. This strongly positions <i>A. muciniphila</i> in the forefront of next-generation candidates for developing novel food or pharma supplements with beneficial effects. Finally, a specific protein present on the outer membrane of <i>A. muciniphila</i>, termed Amuc_1100, could be strong candidate for future drug development. In conclusion, as plants and its related knowledge, known as pharmacognosy, have been the source for designing drugs over the last century, we propose that microbes and microbiomegnosy, or knowledge of our gut microbiome, can become a novel source of future therapies.
New Transfer Learning Techniques for Disparate Label Sets (2015)

Young-Bum Kim, Karl Stratos, Ruhi Sarikaya, Minwoo Jeong. Proceedings of the 53rd Annual Meeting of the Association for Computational Linguistics and the 7th International Joint Conference on Natural Language Processing (Volume 1: Long Papers). 2015.
The polymerization of acrylamide initiated by ceric nitrate‐3‐chloro‐1‐propanol redox systems (1959)

Abstract The polymerization of acrylamide initiated by ceric nitrate and 3‐chloro‐1‐propanol was studied in aqueous solution at 25°C. At constant hydrogen ion and nitrate ion concentration, the rate of polymerization was found to be independent of the ceric ion concentration. The molecular weight of the polymer was very low. To explain the data, a kinetic scheme is proposed whereby ceric ions are responsible for both initiation and termination. The rate constant for oxidative termination was found to be of the order of 10 5 l. mole −1 sec. −1 and varied with the hydrogen ion and the nitrate ion concentrations.
Identification and Nucleotide Sequence of a Gene in Equine Herpesvirus 1 Analogous to the Herpes Simplex Virus Gene Encoding the Major Envelope Glycoprotein gB (1989)

A gene in equine herpesvirus 1 (EHV-1; equine abortion virus) equivalent to the gB glycoprotein gene of herpes simplex virus (HSV) has been identified by DNA hybridization and nucleotide sequencing. A 4.3 kbp EHV-1 PstI-ClaI sequence (0.40 to 0.43 map units) contained an open reading frame flanked by appropriate control elements and was capable of encoding a polypeptide of 980 amino acids. This had 50 to 60% identity over a 617 amino acid conserved region with the gB gene products of HSV and three other alphaherpesviruses, and 20 to 30% identity with those of human cytomegalovirus and Epstein-Barr virus. Analysis of the amino acid sequence predicts a long signal peptide, hydrophobic and hydrophilic domains and N-glycosylation sites, and has identified a probable internal proteolytic cleavage site. The EHV-1 gB open reading frame appears to be overlapped at its 5' end by 135 nucleotides of the 3' end of an upstream open reading frame the potential translation product of which has approximately 50% identity with HSV gene ICP 18.5 and VZV gene 30 products.
Discovery and Scoring of Protein Interaction Subnetworks Discriminative of Late Stage Human Colon Cancer (2008)

We used a systems biology approach to identify and score protein interaction subnetworks whose activity patterns are discriminative of late stage human colorectal cancer (CRC) versus control in colonic tissue. We conducted two gel-based proteomics experiments to identify significantly changing proteins between normal and late stage tumor tissues obtained from an adequately sized cohort of human patients. A total of 67 proteins identified by these experiments was used to seed a search for protein-protein interaction subnetworks. A scoring scheme based on mutual information, calculated using gene expression data as a proxy for subnetwork activity, was developed to score the targets in the subnetworks. Based on this scoring, the subnetwork was pruned to identify the specific protein combinations that were significantly discriminative of late stage cancer versus control. These combinations could not be discovered using only proteomics data or by merely clustering the gene expression data. We then analyzed the resultant pruned subnetwork for biological relevance to human CRC. A number of the proteins in these smaller subnetworks have been associated with the progression (CSNK2A2, PLK1, and IGFBP3) or metastatic potential (PDGFRB) of CRC. Others have been recently identified as potential markers of CRC (IFITM1), and the role of others is largely unknown in this disease (CCT3, CCT5, CCT7, and GNA12). The functional interactions represented by these signatures provide new experimental hypotheses that merit follow-on validation for biological significance in this disease. Overall the method outlines a quantitative approach for integrating proteomics data, gene expression data, and the wealth of accumulated legacy experimental data to discover significant protein subnetworks specific to disease.
Cutting Edge: Differential Chemokine Production by Myeloid and Plasmacytoid Dendritic Cells (2002)

To examine the different roles of myeloid dendritic cells (M-DCs) and plasmacytoid dendritic cells (P-DCs) in the induction and regulation of immune response, we have studied chemokine secretion by freshly isolated DC subsets in response to bacterial, viral, and T cell-derived stimuli. M-DCs selectively produced very high levels of the homeostatic chemokines CC chemokine ligand (CCL)17 and CCL22, while P-DCs produced very little if any. In contrast, the proinflammatory chemokine CCL3 was secreted mostly by P-DCs, whereas CCL4 and CXC chemokine ligand 8 were produced by both subsets. The selective production of CCL17 and CCL22 by M-DCs but not P-DCs was confirmed in vivo by immunohistology on human reactive lymph node sections. The high production of CCR4 ligands by M-DCs suggests their capacity to selectively recruit at sites of inflammation T cells with regulatory properties or with a Th2 phenotype, whereas P-DCs, by preferentially secreting CCR1/CCR5 ligands, would mostly recruit effector T cells and, in particular, Th1-type cells.
DVI—a digital multimedia technology (1989)

A digital presentation technology that manages anything from text to full-motion video has the potential of expanding the usefulness of personal computers, while rendering them less intimidating.
Heparin-Binding Protein (CAP37) Is Internalized in Monocytes and Increases LPS-Induced Monocyte Activation (1998)

Previous studies have shown that the neutrophil-derived heparin-binding protein (HBP), also known as CAP37 or azurocidin, potentiates the LPS-induced release of proinflammatory cytokines (TNF-alpha, IL-1, and IL-6) from isolated human monocytes. To date, the mechanisms by which HBP enhances LPS-induced monocyte activation have not been elucidated, and it is not known whether HBP also increases the LPS-induced production of other bioactive substances. We studied human monocytes activated by recombinant human HBP and LPS and their interaction with the LPS receptor CD14. We hypothesized that the stimulatory effect of HBP on the LPS-induced release of proinflammatory mediators from monocytes was mediated by specific binding of HBP to monocytes, which resulted in an up-regulation of CD14. Our results demonstrated that HBP alone (10 microg/ml) stimulated the production of TNF-alpha from isolated monocytes. In addition, HBP had an additive effect on LPS-induced production of TNF-alpha and PGE2, suggesting a generalized monocyte activation. We used flow cytometry to demonstrate that HBP had a high affinity to monocytes but not to the LPS receptor CD14, and experiments performed at 4 degrees C indicated an energy-dependent step in this process. Confocal microscopy showed that monocytes internalize HBP within 30 min. These data suggest that mechanisms other than increased CD14 expression are responsible for the enhanced release of TNF-alpha or PGE2 in response to HBP and LPS.
Load-balanced clustering of wireless sensor networks (2004)

Wireless sensor networks have potential to monitor environments for both military and civil applications. Due to inhospitable conditions these sensors are not always deployed uniformly ion the area of interest. Since sensors are generally constrained in on-board energy supply, efficient management of the network is crucial to extend the life of the sensors. Sensors' energy cannot support long haul communication to reach a remote command site and thus requires many levels of hops or a gateway to forward the data on behalf of the sensor. In this paper, we propose an algorithm to network these sensors in to well define clusters with less energy-constrained gateway nodes acting as cluster-heads, and balance load among these gateways. Simulation results show how our approach can balance the load and improve the lifetime of the system.
Optimal Control of Sedimentation in Navigation Channels (1999)

Many hydraulic engineering projects concern the control of sedimentation. For some large engineering projects, such as construction of a navigation channel over many kilometers in a shallow estuary, comprehensive control of sedimentation is extremely costly. An optimal-control approach is presented. It determines optimal locations and scheduling of dredging to minimize total cost and to obtain a channel where water depths are not less than specified values. The optimal-control problem is formulated and a method is developed. The optimization problem is solved by the conjugate gradient method, and the gradient of the cost function is calculated by solving the adjoint problem. A simulation study is conducted using a 2D numerical model to demonstrate the method. The significance of the adjoint problem is also illustrated in sensitivity analysis experiments.
Design of Novel Aminoglycoside Derivatives with Enhanced Suppression of Diseases-Causing Nonsense Mutations (2016)

New pseudotrisaccharide derivatives of aminoglycosides that exploit additional interaction on the shallow groove face of the decoding-site rRNA of eukaryotic ribosome were designed, synthesized and biologically evaluated. Novel lead structures (6 and 7 with an additional 7'-OH), exhibiting enhanced specificity to eukaryotic cytoplasmic ribosome, and superior nonsense mutation suppression activity than those of gentamicin, were discovered. The comparative benefit of new leads was demonstrated in four different nonsense DNA-constructs underling the genetic diseases cystic fibrosis, Usher syndrome, and Hurler syndrome.
Anthelmintic Activity of Paraherquamide in Sheep (1990)

Paraherquamide, an oxindole alkaloid metabolite of Penicillium paraherquei, was tested against the common gastrointestinal nematode species of sheep at 0.25, 0.5, 1.0, and 2.0 mg/kg, per os. It was highly efficacious (greater than or equal to 98% reduction) as a single oral treatment dosages greater than or equal to 0.5 mg/kg against adult Haemonchus contortus, Ostertagia circumcincta, Trichostrongylus axei, Trichostrongylus colubriformis and Cooperia curticei, and the L4 stage of Cooperia spp. Noteworthy is the fact the isolate of H. contortus used was ivermectin-resistant and the isolate of T. colubriformis used was ivermectin- and benzimidazole-resistant. This suggests a different mode of action for paraherquamide relative to ivermectin and the benzimidazoles. The adult stage of Oesophagostomum columbianum was the dosage-limiting parasite with 79% efficacy recorded at the highest treatment level (2.0 mg/kg). Extrapolation from the O. columbianum response curve suggests a dosage in excess of 4.0 mg/kg would be required to attain 95% efficacy.
A subset of human monocyte-derived dendritic cells expresses high levels of interleukin-12 in response to combined CD40 ligand and interferon-γ treatment (2000)

Dendritic cells (DCs) may arise from multiple lineages and progress through a series of intermediate stages until fully mature, at which time they are capable of optimal antigen presentation and T-cell activation. High cell surface expression of CD83 is presumed to correlate with full maturation of DCs, and a number of agents have been shown to increase CD83 expression on DCs. We hypothesized that interleukin 12 (IL-12) expression would be a more accurate marker of functionally mature DCs capable of activating antigen-specific T cells. We used combinations of signaling through CD40, using CD40 ligand trimer (CD40L), and interferon gamma to demonstrate that CD83 expression is necessary but not sufficient for optimal production of IL-12 by DCs. Phenotypically mature DCs could be induced to produce high levels of IL-12 p70 only when provided 2 simultaneous stimulatory signals. By intracellular cytokine detection, we determined that only a subset of cells that express high levels of CD80 and CD83 generate large amounts of IL-12. DCs matured with both signals are superior to DCs stimulated with the individual agents in activating antigen-specific T cell in vitro. These findings have important implications regarding the identification, characterization, and clinical application of functionally mature DCs.
Active Sampled-data Controlled Suspension in Automobile with Vibration Manipulation Functions (2016)

The intermittent feedforward function, termed vibration manipulation function (VMF), was proposed for sampled-data control of an actuator to achieve active suspension of an automobile with road profile undulation. The function can manipulate the position and velocity of a sprung mass of a 1DOF quarter car model in every arbitral operational period. Elongation or force of the actuator can be determined from VMF, which works to suppress vibration of the sprung mass without knowing road profile. Since required power of the actuator can be made smaller and intermittently-feedbacked feedforward control is much easier to be realized, proposed method could provide an alternative guiding principle for active suspension of automobile.
Cryptolepis sanguinolenta:Antimuscarinic Properties of Cryptolepine and the Alkaloid Fraction at M1, M2 and M3 Receptors (1992)

From an ethanol extract of the roots of Cryptolepis sanguinolenia the alkaloid fraction and its main constituent cryptolepine were isolated by partitioning at pH 11 and column chromatography using silica gel and chloroform/methanol as eluent. Cryptolepine was identified mainly by EI-MS and 1H/13C-NMR spectroscopy. Cryptolepine (3-30 microM) and the alkaloid fraction of Cryptolepis sanguinolenta (3-10 micrograms/ml) antagonized muscarinic effects at M1 receptors in rabbit vas deferens, M2 receptors in guinea-pig atria, and M3 receptors in guinea-pig ileum. The experiments, using N-methylatropine as reference drug, showed a significant antimuscarinic activity for both cryptolepine and the alkaloid fraction, but no appreciable receptor subtype selectivity (pA2 = 5.00-5.73). Cryptolepine was determined as the antimuscarinic principle of Cryptolepis sanguinolenta. At higher concentrations both materials displayed negative inotropic effects.
Stereoselective synthesis of 1,3-amino alcohols and 1,3-amino ketones (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereoselective synthesis of 1,3-amino alcohols and 1,3-amino ketonesJose Barluenga, Enrique Aguilar, Santos Fustero, Bernardo Olano, and Argimiro L. ViadoCite this: J. Org. Chem. 1992, 57, 4, 1219–1223Publication Date (Print):February 1, 1992Publication History Published online1 May 2002Published inissue 1 February 1992https://pubs.acs.org/doi/10.1021/jo00030a033https://doi.org/10.1021/jo00030a033research-articleACS PublicationsRequest reuse permissionsArticle Views574Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Sox17 is essential for the specification of cardiac mesoderm in embryonic stem cells (2007)

Early steps for cardiac specification are problematic for the study of mammalian embryos, which has favored using pluripotent cells that recapitulate cardiac myogenesis. Furthermore, circuits governing cardiac specification have relevance to the application of ES cells and other cells for heart repair. In mouse teratocarcinoma cells, canonical Wnts that inhibit heart formation in avian or amphibian embryos and explants activate cardiogenesis, paradoxically. Here, we show that the Wnt/beta-catenin pathway also is essential for cardiac myogenesis to occur in ES cells, acting at a gastrulation-like stage, mediating mesoderm formation and patterning (two prerequisites for cardiac myogenesis itself). Among genes associated temporally with this step was Sox17, encoding an endodermal HMG-box transcription factor. Using lentiviral vectors for RNA interference in differentiating ES cells, an essential role for Sox17 was proven in cardiac muscle cell formation. Sox17 short-hairpin RNA suppresses cardiac myogenesis selectively, acting subsequent to mesoderm formation yet before induction of Mesp1 and Mesp2, a pair of related basic helix-loop-helix transcription factors that together are indispensable for creating heart mesoderm. Sox17 short-hairpin RNA blocks cardiac myogenesis non-cell autonomously and impairs the induction of Hex, a homeodomain transcription factor that is known to be required for the production of endoderm-derived heart-inducing factors.