gebhart/patrepeval huggingface dataset:
In Vivo proton spectroscopy and spectroscopic imaging of {1‐13C}‐g1ucose and its metabolic products (1993)

Metabolism of [1-13C]-glucose was studied in situ in cat brain using gradient-enhanced proton-detected heteronuclear spectroscopy. Proton detection of [1-13C]-glucose, [3-13C]-lactate, 4-[13C]-glutamine, 4-[13C]-glutamate and the combined signals 2-[13C]-glutamate/glutamine and 3-[13C]-glutamate/glutamine was achieved, despite the fact that some of the associated proton resonances are close to the water signal. Two-dimensional [1H-13C]-spectra demonstrate the possibility of in situ spectral assignment with 1H sensitivity and 13C resolution. Spectroscopic images of glucose and its metabolic products were also acquired, showing the possibility to study spatial dependence of metabolism.
Conference Report 2001 (2001)

Case Management - Art of Science, CMSA's fourth annual conference, was held in Melbourne in February 2001 with the theme being evaluation. There were 291 delegates at the conference providing representation from all the Australian states and territories, in addition to the international delegates from New Zealand, USA and Sweden.
Building Temporal Models for Gesture Recognition (2000)

This work presents a piecewise linear approximation to non-linear Point Distribution Models for modelling the human hand.The work utilises the natural segmentation of shape space, inherent to the technique, to apply temporal constraints which can be used with CONDENSATION to support multiple hypotheses and quantum leaps through shape space.This paper presents a novel method by which the one-state transitions of the English Language are projected into shape space for tracking and model prediction using a HMM like approach.
Toward Label-Free Super-Resolution Microscopy (2015)

We propose and implement a far-field spectroscopic system for imaging below the diffraction limit without the need for fluorescence labeling. Our technique combines concepts from Stimulated Emission Depletion (STED) microscopy and Femtosecond Stimulated Raman Spectroscopy (FSRS). The FSRS process generates signal through the creation of vibrational coherences, and here we use a toroidal-shaped decoherence pulse to eliminate vibrational signal from the edges of the focal spot. The nonlinear dependence on decoherence pulse power enables subdiffraction imaging. As in STED, the resolution is in theory infinitely small given infinite decoherence pulse power. Here, we first experimentally demonstrate that the photophysical principles behind our super-resolution Raman imaging method are sound. We then prove that addition of the decoherence pulse significantly improves the spatial resolution of our microscope, achieving values beyond the diffraction limit. We discuss future directions for this technique, including methods to reach resolution on the order of ten nanometers.
Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes (2009)

This paper reports significant improvements in the efficacy of sequence-independent amplification and quality of sequencing of viruses with segmented double-stranded RNA (dsRNA) genomes. We demonstrate that most remaining bottlenecks in dsRNA virus genome characterization have now been eliminated. Both the amplification and sequencing technologies used require no previous sequence knowledge of the viral dsRNA, there is no longer a need to separate genome segments or amplicons and the sequence-determined bias observed in cloning has been overcome. Combining very efficient genome amplification with pyrophosphate-based 454 (GS20/FLX) sequencing enabled sequencing of complete segmented dsRNA genomes and accelerated the sequence analysis of the amplified viral genomes. We report the complete consensus sequence of seven viruses from four different dsRNA virus groups, which include the first complete sequence of the genome of equine encephalosis virus (EEV), the first complete sequence of an African horsesickness virus (AHSV) genome determined directly from a blood sample and a complete human rotavirus genome determined from faeces. We also present the first comparison between the complete consensus sequence of a virulent and an attenuated strain of AHSV1. Ultra-deep sequencing (>400-fold coverage) of the AHSV1 reference and attenuated strains revealed different ratios of reassortants in the reference strain and allowed quasispecies detection in the plaque-purified attenuated strain of AHSV1. This approach amounts to a paradigm shift in dsRNA virus research, since it is sensitive and specific enough for comprehensive investigations of the evolution and genetic diversity in dsRNA virus populations.
Cloning and Heterologous Expression of a Second (+)-δ-Cadinene Synthase from Gossypium arboreum (1996)

Screening of a Gossypium arboreum L. cv. Nanking cDNA library resulted in the identification and cloning of a second (+)-delta-cadinene synthase. A probe for the screens was prepared by PCR using primers based on conserved sequences in farnesyl diphosphate cyclases and genomic DNA as a template. This second cDNA clone encodes a protein that is 80% identical to the recently described (+)-delta-cadinene synthases CAD1-C1 and C14 from G. arboreum and maintains a significant degree of homology to the other known mono-, sesqui-, and diterpene synthases. As in the case of CAD1-C1 (+)-delta-cadinene synthase from cultured cotton cells, the synthesis of the second CAD1-A mRNA was induced by treatment of cotton cell suspension cultures with a partially purified elicitor preparation from the phytopathogenic fungus Verticillium dahliae. Expression of CAD1-A mRNA was quantitated with reverse transcription PCR and showed that CAD1-A mRNA was maximally increased 8-fold, 6 h after addition of elicitor. Heterologous expression of this second cDNA produced a 64 kD protein that catalyzed the cyclization of farnesyl diphosphate to (+)-delta-cadinene, the identical product produced by CAD1-C1. The steady-state kinetic parameters of CAD1-A were similar to CAD1-C, showing a Km of 7 mM farnesyl diphosphate and kcat of 0.039 s-1 at 30 degrees C. However, the optimal pH and Mg2+ concentration for CAD1-A activity were significantly higher than those observed for CAD1-C.
Closed-Tube Genotyping with Unlabeled Oligonucleotide Probes and a Saturating DNA Dye (2004)

Genotyping of SNPs and small deletions by melting analysis of an unlabeled probe in the presence of LCGreen I is simple and rapid. Only three unlabeled oligonucleotides (two primers and one probe), a saturating DNA dye, PCR, and a melting instrument are required. The method is closed-tube, does not require fluorescently labeled probes or real-time PCR, and can be completed in <10 min on any instrument capable of monitoring melting curves by fluorescence.
Artificial Tactile Sensing of Position and Slip Speed by Exploiting Geometrical Features (2014)

Rich information about artificial grasps can be obtained using tactile sensing arrays. However, the complexity of the integration and computation inherent to tactile sensor arrays limits their applicability for prosthetic manipulators. We present an artificial ridged skin that detects the position and speed of a slipping object using a single force sensor. The artificial skin features parallel ridges arranged in a nonuniform configuration. An evolutionary algorithm generates distributions of ridges evaluated by the accuracy and resolution of detecting the position and speed of a slipping object. Slip experiments on real skins featuring ridge distributions generated by the evolutionary algorithm show that ridge arrangement is critical for an improved sensing of object position and slip speed. We report ridge patterns that detect object position and speed with errors as low as 10% at slip speeds of up to 60 mm/s. The artificial skin has an average speed sensing resolution of 10 mm/s, an average position sensing resolution of 15 mm, and is robust to various grip conditions, e.g., speed variation, object weight, and contact area. By exploiting the geometrical features of the artificial skin, enhanced tactile information is acquired. The concept opens a promising avenue for robust and energy-efficient tactile sensing systems.
Population Structure and Its Effect on Haplotype Diversity and Linkage Disequilibrium Surrounding the xa5 Locus of Rice (Oryza sativa L.) (2003)

To assess the usefulness of linkage disequilibrium mapping in an autogamous, domesticated species, we have characterized linkage disequilibrium in the candidate region for xa5, a recessive gene conferring race-specific resistance to bacterial blight in rice. This trait and locus have good mapping information, a tractable phenotype, and available sequence data, but no cloned gene. We sampled 13 short segments from the 70-kb candidate region in 114 accessions of Oryza sativa. Five additional segments were sequenced from the adjacent 45-kb region in resistant accessions to estimate the distance at which linkage disequilibrium decays. The data show significant linkage disequilibrium between sites 100 kb apart. The presence of the xa5 resistant reaction in two ecotypes and in accessions with different haplotypes in the candidate region may indicate multiple origins or genetic heterogeneity for resistance. In addition, genetic differentiation between ecotypes emphasizes the need for controlling for population structure in the design of linkage disequilibrium studies in rice.
Involvement of fibroblast growth factor-2 in joint destruction of rheumatoid arthritis patients (1999)

The increase in endogenous FGF-2 levels in the synovial fluid of RA patients may play a role in the joint destruction by inducing osteoclastogenesis.
Simultaneous Identification of Bacterial Virulence Genes by Negative Selection (1995)

An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium.
Inorganic/Organic Hybrid Silica Nanoparticles as a Nitric Oxide Delivery Scaffold (2007)

The preparation and characterization of nitric oxide (NO)-releasing silica particles formed following the synthesis of <i>N</i>-diazeniumdiolate-modified aminoalkoxysilanes are reported. Briefly, an aminoalkoxysilane solution was prepared by dissolving an appropriate amount of aminoalkoxysilane in a mixture of ethanol, methanol, and sodium methoxide (NaOMe) base. The silane solution was reacted with NO (5 atm) to form <i>N</i>-diazeniumdiolate NO donor moieties on the amino-alkoxysilanes. Tetraethoxy- or tetramethoxysilane (TEOS or TMOS) was then mixed with different ratios of <i>N</i>-diazeniumdiolate-modified aminoalkoxysilane (10 - 75 mol%, balance TEOS or TMOS). Finally, the silane mixture was added into ethanol in the presence of an ammonia catalyst to form NO donor silica nanoparticles via a sol-gel process. This synthetic approach allows for the preparation of NO delivery silica scaffolds with remarkably improved NO storage and release properties, surpassing all macromolecular NO donor systems reported to date with respect to NO payload (11.26μmol·mg<sup>-1</sup>), maximum NO release amount (357000 ppb·mg<sup>-1</sup>), NO release half-life (253 min), and NO release duration (101 h). The <i>N</i>-diazeniumdiolate-modified silane monomers and the resulting silica nanoparticles were characterized by <sup>29</sup>Si nuclear magnetic resonance (NMR) spectroscopy, UV-visible spectroscopy, chemiluminescence, atomic force microscopy (AFM), gas adsorption-desorption isotherms, and elemental analysis.
E. M. FORSTER (1979)

E. M. FORSTER Get access RONALD MASON RONALD MASON Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume 26, Issue 1, February 1979, Pages 3-b–3, https://doi.org/10.1093/nq/26-1-3b Published: 01 February 1979
Targeting Myeloid Leukemia Stem Cells (2010)

Recently, several research groups have described cell surface molecules that are selectively or differentially expressed on leukemia stem cells (LSCs) relative to normal tissue. The identification of these antigens suggests that antibody-based diagnostic or therapeutic opportunities may be forthcoming. Indeed, preclinical studies have suggested the utility of targeting such molecules as a means of enhancing leukemia therapy. Here the current understanding of the LSC phenotype is described, and the potential application of antibody-based treatment regimens is discussed.
αdβ2 Integrin Is Expressed on Human Eosinophils and Functions as an Alternative Ligand for Vascular Cell Adhesion Molecule 1 (VCAM-1) (1998)

The beta2 family of integrins, CD11a, CD11b, CD11c, and alphad, are expressed on most leukocytes. We show that the newest member of this family, alphad, is expressed on human eosinophils in peripheral blood, and surface expression can be upregulated within minutes by phorbol ester or calcium ionophore A23187. Culture of eosinophils with interleukin 5 (IL-5) leads to a two- to fourfold increase in alphad levels by 3-7 d without a change in alpha4 integrin expression. Eosinophils isolated from late phase bronchoalveolar lavage fluids express alphad at levels similar to that seen after 3 d of IL-5 culture. Regarding alphadbeta2 ligands, in both freshly isolated and IL-5-cultured eosinophils, as well as alphadbeta2-transfected Chinese hamster ovary cells, alphadbeta2 can function as a ligand for vascular cell adhesion molecule 1 (VCAM-1). This conclusion is based on the ability of monoclonal antibodies to alphad, beta2, or VCAM-1 to block cell attachment in static adhesion assays. In experiments with eosinophils, the relative contribution of alphadbeta2 integrin- mediated adhesion is enhanced after IL-5 culture. These experiments demonstrate that alphadbeta2 is an alternative ligand for VCAM-1, and this integrin may play a role in eosinophil adhesion to VCAM-1 in states of chronic inflammation.
Fluorescence Polarization/Anisotropy in Diagnostics and Imaging (2010)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTFluorescence Polarization/Anisotropy in Diagnostics and ImagingDavid M. Jameson* and Justin A. RossView Author Information Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, 651 Ilalo Street, BSB222, Honolulu, Hawaii 96813* To whom correspondence should be addressed. E-mail: [email protected]Cite this: Chem. Rev. 2010, 110, 5, 2685–2708Publication Date (Web):March 16, 2010Publication History Received4 August 2009Published online16 March 2010Published inissue 12 May 2010https://pubs.acs.org/doi/10.1021/cr900267phttps://doi.org/10.1021/cr900267preview-articleACS PublicationsCopyright © 2010 American Chemical SocietyRequest reuse permissionsArticle Views14763Altmetric-Citations488LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Assays,Fluorescence,Imaging probes,Peptides and proteins,Polarization Get e-Alerts
Inhibition of Early and Late Events of the HIV-1 Replication Cycle by Cytoplasmic Fab Intrabodies against the Matrix Protein, p17 (1997)

This novel strategy of simultaneously blocking early and late events of the HIV-1 life cycle may prove useful in clinical gene therapy approaches for the treatment of HIV-1 infection and AIDS, particularly when combined with genetic or pharmacologic-based strategies that inhibit other HIV-1 target molecules simultaneously.
Prehospital blood transfusion programs (2017)

Zielinski, Martin D. MD; Stubbs, James R. MD; Berns, Kathleen S. MS; Glassberg, Elon MD; Murdock, Alan D. MD; Shinar, Eilat MD; Sunde, Geir Arne MD; Williams, Steve RN; Yazer, Mark H. MD; Zietlow, Scott MD; Jenkins, Donald H. MD Author Information
Efficient Hydrogenation of Ketones Catalyzed by an Iron Pincer Complex (2011)

Reductions made easy: A highly efficient iron catalyst effects the homogeneous hydrogenation of ketones under very mild conditions (see scheme). A mechanism including the insertion of the ketone into the FeH bond, followed by addition of hydrogen to a dearomatized intermediate is proposed.
Multi-index hashing for information retrieval (2002)

We describe a technique for building hash indices for a large dictionary of strings. This technique permits robust retrieval of strings from the dictionary even when the query pattern has a significant number of errors. This technique is closely related to the classical Turan problem for hypergraphs. We propose a general method of multi-index construction by generalizing certain Turan hypergraphs. We also develop an accompanying theory for analyzing such hashing schemes. The resulting algorithms have been implemented and can be applied to a wide variety of recognition and retrieval problems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Production of the M2 protein of influenza A virus in insect cells is enhanced in the presence of amantadine (1993)

Recombinant baculoviruses that express the M2 protein from the genes of either the amantadine-sensitive, influenza A/Ann Arbor/6/60 virus or a laboratory-derived, amantadine-resistant mutant of this virus were constructed. Addition of amantadine or rimantadine at 2 micrograms/ml to cultures of Sf9 cells infected with the recombinant baculoviruses increased the yield of the M2 protein from the amantadine-sensitive virus approximately 10-fold, but did not increase the yield of the M2 protein from the amantadine-resistant virus. Flow cytometry demonstrated that the increased production of M2 in the presence of amantadine resulted in increased cell surface expression of the M2 protein. Pulse-chase experiments indicated that whereas the rate of synthesis of the M2 protein increased in the presence of amantadine, the M2 protein was stable in both the presence and absence of amantadine. Addition of amantadine to Sf9 cells as late as 72 h after infection with the recombinant virus increased the production of M2 protein. These data suggest that the M2 protein exerts some biological activity in Sf9 cells.
Eccentric exercise-induced injury to rat skeletal muscle (1983)

These experiments were designed to study skeletal muscle pathology resulting from eccentric-biased exercise in rats. The effects on the muscles of running on a treadmill on a 0 degrees incline (similar amounts of concentric and eccentric contractions), down a 16 degrees incline (primarily eccentric contractions), and up a 16 degrees incline (primarily concentric contractions) at 16 m . min-1 for 90 min were assessed by following postexercise changes in 1) plasma creatine kinase and lactate dehydrogenase activities, 2) glucose-6-phosphate dehydrogenase (G-6-PDase) activity (bio- and histochemically) in the physiological extensor muscles, and 3) histological appearance of the muscles. The data indicate the following. 1) Whereas all exercise protocols resulted in elevations of plasma enzymes immediately after running, only eccentric exercise caused late phase elevations 1.5-2 days postexercise. 2) Significant increases in muscle G-6-PDase activity, which were always associated with accumulations of mononuclear cells, always occurred within some muscles of each extensor group 1-3 days following downhill and uphill running and did not occur following level running; the increases in activity were usually of lower magnitude in the muscles of uphill runners than in those of downhill runners; the deeply located, predominantly slow-twitch muscles were most affected by both down- and uphill running. 3) Muscle histology demonstrated localized disruption of normal banding patterns of some fibers immediately after exercise and accumulations of macrophages in the interstitium and in some (less than 5%) muscle fibers by 24 h postexercise in the deep slow muscles of the antigravity groups. Although the data generally indicated that eccentric exercise causes greater injury to the muscles, questions remain.
Relationship of Heart Rate Variability to Parasympathetic Effect (2001)

The relationship between HRV and parasympathetic effect is best described by a function in which there is an ascending limb where HRV increases as parasympathetic effect increases until it reaches a plateau level; HRV then decreases as parasympathetic effect increases. Because there is marked interindividual variation in this relationship, differences in HRV between individuals may reflect differences in this relationship and/or differences in autonomic effects.
Non-surgical transpericardial catheter ablation of post-infarction ventricular tachycardia. (2005)

Non-surgical transpericardial approach for catheter-based epicardial radiofrequency ablation of post-infarction left ventricular tachycardia has been described as an alternative and additive procedure to standard endocardial technique for delivery of radiofrequency energy in difficult situations. We report our initial experience with this approach in three patients of post-infarction recurrent ventricular tachycardia, refactory to multiple antiarrhythmic drugs. Ablation was successful in terminating the tachycardia in two and in modifying the circuit to be amenable for control with single antiarrhythmic drug in one patient. There were no serious acute or long-term complications related to the procedure. Epicardial approach is an effective and safe adjunct to standard endocardial ablative technique for patients of post-infarction ventricular tachycardia.