gebhart/patrepeval huggingface dataset:
Expression of the Interleukin-6 Gene Is Constitutive and Not Regulated by Estrogen in Rat Vascular Smooth Muscle Cells in Culture* (1999)

Vascular smooth muscle cells (SMC) are major constituents of the medial layer of blood vessels and are involved in the development of atherosclerotic plaque. SMC secrete copious IL-6 under basal conditions that can be increased by cytokines such as tumor necrosis factor-alpha and interleukin-1beta (IL-1beta). The goal of our studies was to define the role of estrogen in IL-6 production by SMC. In a first series of experiments, the expression of specific messenger RNAs as well as the production of IL-6 bioactivity by rat SMC in culture could be demonstrated in basal and IL-1-stimulated conditions, but was unaffected by estrogen treatment. Different constructs containing deleted or mutated fragments of the human IL-6 promoter driving luciferase or chloramphenicol acetyltransferase reporter gene were then transiently transfected in these cells. A significant basal activity that was increased 2- to 4-fold after IL-1beta stimulation was observed with the total IL-6 promoter. Deletion analysis indicated that the -158/+11 region containing activator protein-1 and cAMP response element sites was apparently the minimal region of IL-6 promoter to confer both constitutive and IL-1-inducible activities. Site-directed mutagenesis experiments suggest that basal activity is dependent upon the promoter sequence -158 to -112 containing the nuclear factor (NF)-IL6(-153) and Sp1 sites, whereas IL-1beta stimulation would depend on the residual -112 nucleotides containing NF-IL6(-75) and NF-kappaB sites. In contrast to the down-regulation of IL-6 expression by estrogen described in osteoblasts, ethinyl estradiol as well as 17beta-estradiol did not influence stimulated IL-6 activity in our experimental conditions whatever the construct tested, even when either estrogen receptor alpha or beta was overexpressed. Thus, the atheroprotective properties of estrogen are probably not mediated through the regulation of IL-6 production by SMC.
Molecular characterization of a slowly gating human hyperpolarization-activated channel predominantly expressed in thalamus, heart, and testis (1999)

Rhythmic activity of neurons and heart cells is endowed by pacemaker channels that are activated by hyperpolarization and directly regulated by cyclic nucleotides (termed HCN channels). These channels constitute a multigene family, and it is assumed that the properties of each member are adjusted to fit its particular function in the cell in which it resides. Here we report the molecular and functional characterization of a human subtype hHCN4. hHCN4 transcripts are expressed in heart, brain, and testis. Within the brain, the thalamus is the predominant area of hHCN4 expression. Heterologous expression of hHCN4 produces channels of unusually slow kinetics of activation and inactivation. The mean potential of half-maximal activation (V(1/2)) was -75.2 mV. cAMP shifted V(1/2) by 11 mV to more positive values. The hHCN4 gene was mapped to chromosome band 15q24-q25. The characteristic expression pattern and the sluggish gating suggest that hHCN4 controls the rhythmic activity in both thalamocortical neurons and pacemaker cells of the heart.
Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysis (2003)

This study investigated regional variations in the contribution made by different human papilloma (HPV) types to invasive cervical cancer (ICC). A total of 85 studies using polymerase chain reaction to estimate HPV prevalence in ICC were identified. Data on HPV prevalence were extracted separately for squamous cell carcinoma (SCC) and for adeno- and adenosquamous-carcinoma (ADC). A total of 10 058 cases (8550 SCC, 1508 ADC) were included in pooled analyses. The most common HPV types in ICC were, in order of decreasing prevalence, HPV16, 18, 45, 31, 33, 58, 52, 35, 59, 56, 6, 51, 68, 39, 82, 73, 66 and 70. In SCC, HPV16 was the predominant type (46-63%) followed by HPV18 (10-14%), 45 (2-8%), 31 (2-7%) and 33 (3-5%) in all regions except Asia, where HPV types 58 (6%) and 52 (4%) were more frequently identified. In ADC, HPV prevalence was significantly lower (76.4%) than in SCC (87.3%), and HPV18 was the predominant type in every region (37-41%), followed by 16 (26-36%) and 45 (5-7%). The overall detection of HPV DNA was similar in different regions (83-89%). A majority of ICC was associated with HPV16 or 18 in all regions, but approximately a quarter of all ICC cases were associated with one of 16 other HPV types, their distribution varying by region.
Nuclear localization of the adenovirus DNA-binding protein: requirement for two signals and complementation during viral infection. (1989)

The adenovirus DNA-binding protein (DBP) is an abundant multifunctional protein located primarily in the nuclei of infected cells. To define sequences involved in nuclear transport of DBP, a series of point and small deletion mutants were constructed via oligonucleotide-directed mutagenesis. Two short stretches of basic amino acids located in the amino-terminal domain (amino acids 42 to 46 and 84 to 89) were identified. Their importance, however, depended on the context in which DBP was expressed. Disruption of either site prevented nuclear localization after transient expression in transfected 293 cells, implying that two nuclear localization signals are necessary for transport of this nuclear protein. In contrast, the mutant DBPs synthesized during viral infection were located either primarily in the nucleus or in the nucleus and cytoplasm, depending on the mutation and the stage of the viral infection. Thus, the nuclear localization defect could be complemented by viral infection, perhaps through the interaction of the mutant polypeptide with a virus-encoded or -induced factor(s).
Engineered Thio-Trastuzumab-DM1 Conjugate with an Improved Therapeutic Index to Target Human Epidermal Growth Factor Receptor 2–Positive Breast Cancer (2010)

The engineered thioTMAb-mpeo-DM1 with broadened therapeutic index represents a promising antibody drug conjugate for future clinical development of HER2-positive targeted breast cancer therapies.
Effect of mismatch strain on band gap in III-V semiconductors (1985)

Interfacial elastic strain induced by the lattice parameter mismatch between epilayer and substrate results in significant energy–band-gap shifts for III-V alloys. The epilayers used in this study are GaxIn1−xAs on (100) InP and GaxIn1−xP on (100) GaAs prepared by organometallic vapor phase epitaxy. For layer thicknesses between 1 and 1.5 μm, and Δas.f./a0≤3.5×10−3 the misfit strain is assumed to be accommodated elastically. The energy–band-gap shifts are determined by comparing the photoluminescence peak energies of the epilayers with the best experimental relation of band gap versus composition for unstrained layers. A calculation of the energy–band-gap shift due to biaxial stress made for GaxIn1−xAs is found to agree with the photoluminescence measurements. In addition, a comparison of the energy–band-gap shift for GaxIn1−xP shows a clearly different dependency for tensile and compressive strain, in good agreement with calculated results.
Attentive Visual Tracking and Trajectory Estimation for Dynamic Scene Segmentation (1994)

FACULTY OF ENGINEERING AND APPLIED SCIENCE ELECTRONICS AND COMPUTER SCIENCE Doctor of Philosophy ATTENTIVE VISUAL TRACKING AND TRAJECTORY ESTIMATION FOR DYNAMIC SCENE SEGMENTATION by Jonathan Michael Roberts Intelligent Co-Pilot Systems (ICPS) offer the next challenge to vehicle-highway automation. The key to ICPSs is the detection of moving objects (other vehicles) from the moving observer using a visual sensor. The aim of the work presented in this thesis was to design and implement a feature detection and tracking strategy that is capable of tracking image features independently, in parallel, and in real-time and to cluster/segment features utilising the inherent temporal information contained within feature trajectories. Most images contain areas that are of little or no interest to vision tasks. An attentive, data-driven, approach to feature detection and tracking is proposed which aims to increase the efficiency of feature detection and tracking by focusing attention onto relevan...
Cutinase, a lipolytic enzyme with a preformed oxyanion hole (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCutinase, a lipolytic enzyme with a preformed oxyanion holeChrislaine Martinez, Anne Nicolas, Herman van Tilbeurgh, Marie Pierre Egloff, Claire Cudrey, Robert Verger, and Christian CambillauCite this: Biochemistry 1994, 33, 1, 83–89Publication Date (Print):January 11, 1994Publication History Published online1 May 2002Published inissue 11 January 1994https://pubs.acs.org/doi/10.1021/bi00167a011https://doi.org/10.1021/bi00167a011research-articleACS PublicationsRequest reuse permissionsArticle Views745Altmetric-Citations148LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Brightness distribution in rf‐sputtered zns:tbf3 electroluminescent panels (1985)

Abstract Thin‐film ZnS:TbF 3 was deposited by sputtering and a thin‐film electroluminescent panel having a display area of 34 mm × 34 mm was fabricated. The brightness distribution was investigated and film thickness variation and distribution of Tb luminescence centers were studied in conjunction with the brightness. It was found that uniformity of the brightness increases as the applied voltage increases and frequency decreases. Relative brightness deviation ( B max ‐ B min ,)/ B a , where B max , B min and B a indicate maximum, minimum and average brightness, respectively, of the film was 13 to 23% when it was driven by 5 kHz, 40‐V voltage. It was observed that brightness distribution depends on film thickness of the ZnS:TbF 3 layer and that the thinner part has higher brightness. Based on the consideration that thickness variation causes irregular field intensity as well as the conduction charge distribution, and results in brightness variation, we calculated the brightness deviation and found that it agreed well with the experimental results. It was also demonstrated that Tb ions were scattered uniformly in the sputtered ZnS film, which was proved to have uniform luminescence center distribution.
Expression of human platelet glycoprotein Ib alpha in transgenic mice. (1993)

Platelets are cytoplasmic fragments of megakaryocytes and, therefore, their membrane proteins cannot be manipulated by expression methods in culture. To overcome this limitation, we have expressed human glycoprotein (GP) Ib alpha in transgenic mouse megakaryocytes and found that it was present on the surface of platelets associated with the mouse GP Ib beta subunit. This finding demonstrates that assembly of the heterooligomeric GP Ib complex occurs through mechanisms conserved across species. In contrast, the receptor function of GP Ib exhibited restricted species specificity, since only the chimeric complex containing human GP Ib alpha bound human von Willebrand factor and supported platelet aggregation mediated by ristocetin. These studies demonstrate the transgenic engineering of a platelet adhesion receptor under control of the human GP Ib alpha promoter and illustrate a new approach to manipulate platelet receptors and study structure-function relationships in hemostasis and thrombosis.
Dissociation of Human Copper-Zinc Superoxide Dismutase Dimers Using Chaotrope and Reductant (2004)

The dissociation of apo- and metal-bound human copper-zinc superoxide dismutase (SOD1) dimers induced by the chaotrope guanidine hydrochloride (GdnHCl) or the reductant Tris(2-carboxyethyl)phosphine (TCEP) has been analyzed using analytical ultracentrifugation. Global fitting of sedimentation equilibrium data under native solution conditions (without GdnHCl or TCEP) demonstrate that both the apo- and metal-bound forms of SOD1 are stable dimers. Sedimentation velocity experiments show that apo-SOD1 dimers dissociate cooperatively over the range 0.5-1.0 M GdnHCl. In contrast, metal-bound SOD1 dimers possess a more compact shape and dissociate at significantly higher GdnHCl concentrations (2.0-3.0 M). Reduction of the intrasubunit disulfide bond within each SOD1 subunit by 5-10 mM TCEP promotes dissociation of apo-SOD1 dimers, whereas the metal-bound enzyme remains a stable dimer under these conditions. The Cys-57 --> Ser mutant of SOD1, a protein incapable of forming the intrasubunit disulfide bond, sediments as a monomer in the absence of metal ions and as a dimer when metals are bound. Taken together, these data indicate that the stability imparted to the human SOD1 dimer by metal binding and the formation of the intrasubunit disulfide bond are mediated by independent molecular mechanisms. By combining the sedimentation data with previous crystallographic results, a molecular explanation is provided for the existence of different SOD1 macromolecular shapes and multiple SOD1 dimeric species with different stabilities.
Pilot Scale Continuous Microwave Dry-Media Reactor - Part 1: Design and Modeling (2000)

This work presents a new pilot plant continuous microwave dry-media reactor (CMDR) for industrial chemical applications. The CMDR consists of a 6 kW conveyor microwave oven with a subsequent hot air holding section. This microwave reactor has been designed for dry media or solvent-free reactions and can treat through-put in the range of 10–100 kg/h. The microwave heating behavior on the small scale is analyzed and the results are used to estimate the electromagnetic field requirements on the large scale. The temperature and the electric field distribution in the reactor are modeled and experimentally validated. In the second part of this study, a “waxy” esterification reaction was investigated with the CMDR. The reaction time needed for 95% yield was reduced by a factor of 20–30 compared to conventional industrial reactors. This was due to the more homogeneous heat transfer of microwaves, which allows a higher bulk temperature to be reached.
ZCZ-CDMA and OFDMA using M-QAM for broadband wireless communications: Research Articles (2004)

Drug development targeting fibroblast growth factor receptors (FGFRs) represents an emerging theme in the field of medicinal chemistry. Considering the fact that most of the currently identified FGFR agonists are long chain peptides with limited stability, the discovery of novel non-peptide FGFR ligands is still highly demanded. A linear one-bead-one-compound peptoid (oligomers of N-substituted glycine units) library with a theoretical diversity of 10<sup>6</sup> was designed and synthesized. Microarray-based screening led to the identification of four hit sequences <b>1</b>-<b>4</b> as FGFR1α ligands, which were further confirmed using both solution-phase and solid-phase binding assays. Western blot results indicated that peptoids <b>2</b>-<b>4</b> activated FGFR signaling pathways, resulting in increased levels of p-Akt and p-ERK in different cell lines. Our work discovered novel peptoid ligands as FGFR agonists, shedding new light on FGFR-based drug discovery.
Expression of T helper 17 cells and interleukin 17 in lupus nephritis patients (2017)

Modern data suggest that interleukin 17 (Il-17) and Il-17 producing cells play an important role in the pathogenesis of lupus nephritis (LN). It was reported that T helper 17 migrate to the kidney and contribute to inflammatory processes. To measure the frequencies of peripheral blood T helper-17 (Th17) cells and IL-17 concentration in urine in systemic lupus erythematosus (SLE) patients and to study their association with disease activity and renal involvement. This work included 45 patients; 36 females and 9 males with 20 matching controls. Patients were subjected to clinical and laboratory assessments with emphasis on renal affection. The SLE disease activity index was also calculated. Expression of Th-17 cells in peripheral blood was measured by flow cytometry via the combined expression of CD3, IL-23R, intracellular IL-22, IL-17 and urinary IL-17 was measured by ELISA. The patients' mean age was 26.5 ± 7.6 years and disease duration was 6.03 ± 4.3 years. Th17 expression and urinary IL-17 were significantly higher in patients than controls (p = 0.04 and p < 0.001 respectively). Th17 cell frequencies and IL-17 levels significantly correlated with renal biopsy classification for LN (p = 0.02 and p = 0.04 respectively). Th17 cell expression and IL-17 levels were comparable in SLE patients with and without LN. Th17 cell frequencies significantly correlated with serum creatinine, SLEDAI and inversely with C3 (p = 0.003), while IL-17 significantly correlated with CD3 cells, proteinuria and erythrocyte sedimentation rate. Peripheral blood Th17 cell frequencies and IL-17 in urine are highly linked to LN in SLE and are promising markers of disease activity in LN. Both are valuable targets for future therapeutic applications.
Predictors of longterm outcome in ankylosing spondylitis. (2003)

Much of the variability in disease severity in AS remains unexplained. All but one of the factors associated with outcome in this study are inherent. This suggests that genetic factors have a greater influence than environmental factors on radiological progression and disability in AS. It may, however, be possible to improve longterm functional outcome in AS by targeting high risk individuals early in the disease course with more aggressive management strategies and encouraging smoking cessation in all patients with AS.
Spectrum and mode of action of azithromycin (CP-62,993), a new 15-membered-ring macrolide with improved potency against gram-negative organisms (1987)

The macrolide antibiotic azithromycin (CP-62,993; 9-deoxo-9a-methyl-9a-aza-9a-homoerythromycin A; also designated XZ-450 [Pliva Pharmaceuticals, Zagreb, Yugoslavia]) showed a significant improvement in potency against gram-negative organisms compared with erythromycin while retaining the classic erythromycin spectrum. It was up to four times more potent than erythromycin against Haemophilus influenzae and Neisseria gonorrhoeae and twofold more potent against Branhamella catarrhalis, Campylobacter species, and Legionella species. It had activity similar to that of erythromycin against Chlamydia spp. Azithromycin was significantly more potent versus many genera of the family Enterobacteriaceae; its MIC for 90% of strains of Escherichia, Salmonella, Shigella, and Yersinia was less than or equal to 4 micrograms/ml, compared with 16 to 128 micrograms/ml for erythromycin. Azithromycin inhibited the majority of gram-positive organisms at less than or equal to 1 micrograms/ml. It displayed cross-resistance to erythromycin-resistant Staphylococcus and Streptococcus isolates. It had moderate activity against Bacteroides fragilis and was comparable to erythromycin against other anaerobic species. Azithromycin also demonstrated improved bactericidal activity in comparison with erythromycin. The mechanism of action of azithromycin was similar to that of erythromycin since azithromycin competed effectively for [14C]erythromycin ribosomebinding sites.
Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma (2009)

Data of this study suggest that Cripto-1 overexpression is connected with the tumorigenesis and progression of NPC, lentivector-mediated RNAi might be feasible for the inhibition of the growth and invasion of NPC.
Resistance of chimpanzee T cells to human immunodeficiency virus type 1 Tat-enhanced oxidative stress and apoptosis (1996)

CD4+ T-cell depletion in AIDS patients involves induction of apoptosis in human immunodeficiency virus (HIV)-infected and noninfected T cells. The HIV type 1 (HIV-1)-transactivating protein Tat enhances apoptosis and activation-induced cell death (AICD) of human T cells. This effect is mediated by the CD95 (APO-1/Fas) receptor-CD95 ligand (CD95L) system and may be linked to the induction of oxidative stress by Tat. Here we show that HIV-1 Tat-induced oxidative stress is necessary for sensitized AICD in T cells caused by CD95L expression. Tat-enhanced apoptosis and CD95L expression in T cells are inhibited by neutralizing anti-Tat antibodies, antioxidants, and the Tat inhibitor Ro24-7429. Chimpanzees infected with HIV-1 show viral replication resembling early infection in humans but do not show T-cell depletion or progression towards AIDS. The cause for this discrepancy is unknown. Here we show that unlike Tat-treated T cells in humans, Tat-treated chimpanzee T cells do not show downregulation of manganese superoxide dismutase or signs of oxidative stress. Chimpanzee T cells are also resistant to Tat-enhanced apoptosis, AICD, and CD95L upregulation.
Glomerular Mesangial Cell-specific Transactivation of Matrix Metalloproteinase 2 Transcription Is Mediated by YB-1 (1997)

Mesangial cell (MC) activation plays a pivotal role in the development of the end stage sclerotic lesion characteristic of most forms of chronic glomerular disease. We have previously demonstrated that MC activation is directly linked to high level expression of the matrix metalloproteinase-2 (MMP-2) enzyme (Turck, J., Pollock, A. S., Lee, L., Marti, H.-P., and Lovett, D. H. (1996) <i>J. Biol. Chem.</i> 25, 15074–15083), the transcription of which is regulated in a tissue-specific fashion. Recent studies (Harendza, S., Pollock, A., Mertens, P. R., and Lovett, D. H. (1995) <i>J. Biol. Chem.</i> 270, 18786–18796) delineated a strong cis-acting enhancer element, designated MMP-2 RE1, within the 5′-flanking region of the rat MMP-2 gene. Gel shift, DNA footprint, and transcriptional analyses mapped the enhancer element to a unique 40-base pair (bp) sequence located at −1322 to −1282 bp relative to the translational start site. Bromodeoxyuridine-substituted UV cross-linking of the 40-bp enhancer element with MC nuclear extracts yielded a single protein of 52 kDa, while Southwestern blot analysis with MMP-2 RE1 demonstrated three hybridizing nuclear proteins of 52, 62, and 86 kDa size. Screening of a human MC cDNA expression library with MMP-2 RE1 exclusively yielded clones with the identical sequence of the transcription factor YB-1. Western blot and supershift gel analysis of MC nuclear extracts with an anti-YB-1 antibody confirmed the presence of YB-1 within the shifted complex. Examination of the MMP-2 RE1 sequence revealed an incomplete Y-box sequence (CTGCTGGGCAAG), which specifically interacted with recombinant YB-1 on DMS protection footprinting analysis. YB-1 protein preferentially bound the single-stranded components of the 40-bp MMP-2 RE1 and, with increasing concentrations, formed multimeric complexes. Co-transfection of YB-1 in MC increased the enhancer activity within the context of the native MMP-2 promoter, while transfection of non-MMP-2-synthesizing glomerular epithelial cells with YB-1 led to transcriptional suppression. This study indicates that YB-1 is a major, cell type-specific transactivator of MMP-2 transcription by glomerular mesangial cells.