gebhart/patrepeval huggingface dataset:
Leu110Pro substitution in the RhD polypeptide is responsible for the DVII category blood group phenotype (1995)

The nucleotide sequence of the RhD transcripts from the reticulocytes of three unrelated variants with the DVII category blood group phenotype has been determined. Our results indicate that the expression of the low frequency antigen Rh40 and the lack of epD8 at the surface of these variant RhD positive red cells are associated with a single point mutation, T329C, in exon 2 of the RHD gene. This nucleotide polymorphism results in a leucine to proline substitution at amino acid position 110 of the RhD polypeptide.
TCP Veno: TCP enhancement for transmission over wireless access networks (2003)

Wireless access networks in the form of wireless local area networks, home networks, and cellular networks are becoming an integral part of the Internet. Unlike wired networks, random packet loss due to bit errors is not negligible in wireless networks, and this causes significant performance degradation of transmission control protocol (TCP). We propose and study a novel end-to-end congestion control mechanism called TCP Veno that is simple and effective for dealing with random packet loss. A key ingredient of Veno is that it monitors the network congestion level and uses that information to decide whether packet losses are likely to be due to congestion or random bit errors. Specifically: (1) it refines the multiplicative decrease algorithm of TCP Reno-the most widely deployed TCP version in practice-by adjusting the slow-start threshold according to the perceived network congestion level rather than a fixed drop factor and (2) it refines the linear increase algorithm so that the connection can stay longer in an operating region in which the network bandwidth is fully utilized. Based on extensive network testbed experiments and live Internet measurements, we show that Veno can achieve significant throughput improvements without adversely affecting other concurrent TCP connections, including other concurrent Reno connections. In typical wireless access networks with 1% random packet loss rate, throughput improvement of up to 80% can be demonstrated. A salient feature of Veno is that it modifies only the sender-side protocol of Reno without changing the receiver-side protocol stack.
Bromodomain and extra-terminal domain bromodomain inhibition prevents synovial inflammation via blocking IκB kinase–dependent NF-κB activation in rheumatoid fibroblast-like synoviocytes (2015)

This study implicates BET Brds as important regulators of IκB kinase/NF-κB-mediated synovial inflammation of RA and identifies BET proteins as novel therapeutic targets in inflammatory arthritis.
Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle (1991)

We have used an antisense strategy to effectively disrupt the expression of two genes encoding myofilament proteins present in C. elegans body wall muscles. DNA segments from the unc-22 and unc-54 genes have been placed in reverse orientation in vectors designed to produce RNA in body wall muscles. When the resulting plasmids are injected into oocytes, progeny with defects in muscle function are produced. These animals have phenotypes consistent with reduction and/or elimination of function of the gene to which antisense RNA has been produced: twitching and disorganization of muscle filaments for the unc-22 antisense constructs and lack of muscle tone, slow movement, and egg laying defects for the unc-54 antisense constructs. A fraction of the affected animals transmit the defective-muscle trait to subsequent generations. In these cases the transforming DNA is present at high copy number and cosegregates with the observed muscle defects. We have examined several of the unc-22 antisense plasmid transformed lines to determine the mechanistic basis for the observed phenotypes. The RNA product of the endogenous unc-22 locus is present at normal levels and this RNA is properly spliced in the region homologous to the antisense RNA. No evidence for modification of this RNA by deamination of adenosine to inosine was found. In affected animals the level of protein product from the endogenous unc-22 locus is greatly reduced. Antisense RNA produced from the transforming DNA was detected and was much more abundant than 'sense' RNA from the endogenous locus. These data suggest that the observed phenotypes result from interference with a late step in gene expression, such as transport into the cytoplasm or translation.
Reconstructing the ubiquitin network - cross-talk with other systems and identification of novel functions (2009)

Using these representations, evidence is provided for the functional diversification of components such as SUMO-dependent Ub-ligases. We also identify novel components of SCF (Skp1-cullin-F-box)-dependent complexes, receptors in the ERAD (endoplasmic reticulum associated degradation) system and a key role for Sus1 in coordinating multiple Ub-related processes in chromatin dynamics. We present evidence for a major impact of the Ub-system on large parts of the proteome via its interaction with the transcription regulatory network. Furthermore, the dynamics of the Ub-network suggests that Ub and SUMO modifications might function cooperatively with transcription control in regulating cell-cycle-stage-specific complexes and in reinforcing periodicities in gene expression. Combined with evolutionary information, the structure of this network helps in understanding the lineage-specific expansion of SCF complexes with a potential role in pathogen response and the origin of the ERAD and ESCRT systems.
Studies on the Synthesis of 4, 6-Diaminoquinoline Derivatives. I (1961)

4, 6-Diaminoquinoline was derived to 4-amino-6-hydrazinoquinoline and this was condensed with various ketones and aldehydes to form the hydrazones listed in Table I. Indole cyclization reaction of acetone hydrazone (X) by the Fischer process afforded the corresponding (XVI) as colorless needles, m.p. 117°, which transited to (XVII) on being heated with 25% alkali hydroxide. (XVII) is the indole-cyclized product corresponding to (X) but its structure could be either (XVIIa) or (XVIIb). In this connection, Huisgen carried out indole cyclization of acetone 6-quinolylhydrazone by the Fischer process and proposed the structural formula (XV) for the product. This structure (XV) seems appropriate in view of the naphthoid activity of quinoline but it cannot be concluded from this fact that the indole-cyclization product of (X) possessing an amino group in 4-position must necessarily be (XVIIa). Since the yield of the cyclization product of (X) was unsatisfactory (29%), (XII) was submitted to indole-cyclization reaction by Huisgen's process in order to compare ultraviolt absorption spectra, and two kinds of products were obtained; one of colorless prisms, m.p. 198°, and the other of pale yellow needles, m.p. 268° The latter was found to be the double salt of the former with zinc chloride and its heating with 25% alkali hydroxide solution afforded the compound of m.p. 198°. The ultraviolet absorption spectra of these compounds are compared in Fig. 1 with those of (XV), (XVII), and 3H-pyrrolo [3, 2-f]quinoline, presumed by Horner, and there is a great possibility that (XVII) takes the pyrroloquinoline form of a similar type and an angular configuration like (XV). Confirmation of this point must await further detailed work.
Biometric Authentication Using Noisy Electrocardiograms Acquired by Mobile Sensors (2016)

Electrocardiogram (ECG) signals from mobile sensors are expected to increase the availability of authentication in the emerging wearable device industry. However, mobile sensors provide a relatively lower quality signal than the conventional medical devices. This paper proposes a practical authentication procedure for ECG signals that collected via one-chip-solution mobile sensors. We designed a cascading bandpass filter for noise cancellation and suggest eight fiducial features. For classification-based authentication, we use the radial basis function kernel-based support vector machine showing the best performance among nine classifiers through experimental comparisons. In spite of noisy ECG signals in mobile sensors, we achieved 4.61% of the equal error rate (EER) on a single heartbeat, and 1.87% of EER on 15 s testing time on 175 subjects, which is a reasonable result and supports the usability of low-cost ECGs for biometric authentication.
Fluorine-Substituted Cyclofenil Derivatives as Estrogen Receptor Ligands:  Synthesis and Structure−Affinity Relationship Study of Potential Positron Emission Tomography Agents for Imaging Estrogen Receptors in Breast Cancer (2006)

In a search for estrogen receptor (ER) ligands to be radiolabeled with fluorine-18 for imaging of ER-positive breast tumors with positron emission tomography (PET), we investigated cyclofenil analogues substituted at the C3 or C4 position of the cyclohexyl group. McMurry coupling of 4,4'-dihydroxybenzophenone with various ketones produced key cyclofenil intermediates, from which C3 and C4 substituents containing alkyl and various oxygen or fluorine-substituted alkyl groups were elaborated. Binding assays to both ERalpha and ERbeta revealed that the C3 site is more tolerant of steric bulk and polar groups than the C4 site, consistent with a computational model of the ERalpha ligand binding pocket. Fluorine substitution is tolerated very well at some sites, giving some compounds having affinities comparable to or higher than that of estradiol. These fluoro and fluoroalkyl cyclofenils merit further consideration as fluorine-18 labeled ER ligands for PET imaging of ERs in breast tumors.
Preparation of immobilized monomeric actin and its use in the isolation of protease‐free and ribonuclease‐free pancreatic deoxyribonuclease I (1989)

A procedure is described for the immobilization of monomeric actin so that about 30% of the immobilized protein is competent to bind the monomeric-actin-binding proteins bovine pancreatic deoxyribonuclease I and chicken villin. The intact tertiary structure of the immobilized actin is required to bind these proteins. Using this resin, a method has been developed for the affinity purification of pancreatic deoxyribonuclease I on a reusable actin column. It involves the binding of deoxyribonuclease I to immobilized actin, extensive washing of the column, followed by elution of the bound deoxyribonuclease I with 10 M formamide. After removal of the formamide, the deoxyribonuclease I has a higher specific activity than the starting material and contained no detectable protease or ribonuclease contamination. This preparation should find considerable application in molecular genetic studies where the enzyme is needed free of these particular contaminants. The affinity column should also be useful for the isolation of other, physiologically relevant, monomeric-actin-binding proteins.
Hydrogen bonding in polymer blends. 4. Blends involving polymers containing methacrylic acid and vinylpyridine groups (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrogen bonding in polymer blends. 4. Blends involving polymers containing methacrylic acid and vinylpyridine groupsJoon Y. Lee, Paul C. Painter, and Michael M. ColemanCite this: Macromolecules 1988, 21, 4, 954–960Publication Date (Print):April 1, 1988Publication History Published online1 May 2002Published inissue 1 April 1988https://pubs.acs.org/doi/10.1021/ma00182a019https://doi.org/10.1021/ma00182a019research-articleACS PublicationsRequest reuse permissionsArticle Views1572Altmetric-Citations291LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
RNAs in the Sera of Persian Gulf War Veterans Have Segments Homologous to Chromosome 22q11.2 (1999)

Reverse transcriptase PCR (RT-PCR) was used for polyribonucleotide assays with sera from deployed Persian Gulf War veterans with the Gulf War Syndrome and a cohort of nonmilitary controls. Sera from veterans contained polyribonucleotides (amplicons) that were obtained by RT-PCR and that ranged in size from 200 to ca. 2,000 bp. Sera from controls did not contain amplicons larger than 450 bp. DNA sequences were derived from two amplicons unique to veterans. These amplicons, which were 414 and 759 nucleotides, were unrelated to each other or to any sequence in gene bank databases. The amplicons contained short segments that were homologous to regions of chromosome 22q11.2, an antigen-responsive hot spot for genetic rearrangements. Many of these short amplicon segments occurred near, between, or in chromosome 22q11.2 Alu sequences. These results suggest that genetic alterations in the 22q11.2 region, possibly induced by exposures to environmental genotoxins during the Persian Gulf War, may have played a role in the pathogenesis of the Gulf War Syndrome. However, the data did not exclude the possibility that other chromosomes also may have been involved. Nonetheless, the detection of polyribonucleotides such as those reported here may have application to the laboratory diagnosis of chronic diseases that have a multifactorial etiology.
In vivoeffects of targeting CD79b with antibodies and antibody-drug conjugates (2009)

Antibodies directed against B cells are in use for the treatment of non-Hodgkin's lymphoma and autoimmune disorders. The B-cell-restricted surface antigen CD79b, a signaling component of the B-cell receptor, has been shown as a promising antibody target in mouse efficacy models of systemic lupus erythematosus. Anti-CD79b antibody-drug conjugates (ADC), cytotoxic drugs linked through specialized chemical linkers to antibodies, are effective in mouse xenograft models of non-Hodgkin's lymphoma. We were interested in evaluating the systemic effects of anti-CD79b antibodies and ADCs in normal animals as a step toward the development of these molecules as therapeutics. As we were unable to identify any cell surface binding anti-human CD79b antibodies that were cross-reactive to other species, we developed an antibody to cynomolgus monkey (Macaca fascicularis) CD79b (anti-cyCD79b). The anti-cynomolgus antibody, anti-cyCD79b (10D10), and the maytansine (tubulin inhibitor)-conjugated ADC, anti-cyCD79b (10D10)-MCC-DM1, were administered to cynomolgus monkeys at approximately 30 mg/kg (6,000 microg DM1/m(2)) for two doses 3 weeks apart. Anti-cyCD79b and anti-cyCD79b-MCC-DM1 resulted in peripheral blood B-cell depletion of approximately 65% and approximately 94%, respectively. In addition, anti-cyCD79b-MCC-DM1 resulted in near-complete absence of splenic germinal centers, an observation supporting an effect on dividing B cells. Both molecules were well tolerated, with minimal findings for the antibody and findings for the ADC limited to the lymphoid and hematopoietic systems, liver, and peripheral nerves. These preclinical data suggest that targeting CD79b with antibodies or ADCs may provide safe and effective therapies for B-cell malignancies and autoimmune diseases.
How I use hydroxyurea to treat young patients with sickle cell anemia (2010)

Hydroxyurea has many characteristics of an ideal drug for sickle cell anemia (SCA) and provides therapeutic benefit through multiple mechanisms of action. Over the past 25 years, substantial experience has accumulated regarding its safety and efficacy for patients with SCA. Early proof-of-principle studies were followed by prospective phase 1/2 trials demonstrating efficacy in affected adults, then adolescents and children, and more recently infants and toddlers. The phase 3 National Heart, Lung and Blood Institute-sponsored Multicenter Study of Hydroxyurea trial proved clinical efficacy for preventing acute vaso-occlusive events in severely affected adults. Based on this cumulative experience, hydroxyurea has emerged as an important therapeutic option for children and adolescents with recurrent vaso-occlusive events; recent evidence documents sustained long-term benefits with prevention or reversal of chronic organ damage. Despite abundant evidence for its efficacy, however, hydroxyurea has not yet translated into effective therapy for SCA. Because many healthcare providers have inadequate knowledge about hydroxyurea, patients and families are not offered treatment or decline because of unrealistic fears. Limited support for hydroxyurea by lay organizations and inconsistent medical delivery systems also contribute to underuse. Although questions remain regarding its long-term risks and benefits, current evidence suggests that many young patients with SCA should receive hydroxyurea treatment.
Bend performance of leakage channel fibers (2008)

Bend loss of the first three modes of leakage channel fibers with various designs are studied using finite element method. It is found that very low bend loss at small bend radius can be achieved with large d/Delta. It is also found that best differential mode loss is achieved at large bend radius. It is further found that 2nd order mode loss becomes 9.1 times of fundamental mode loss at small bend radius for the bend orientation where the bending plane crosses centers of two holes and independent of d/Delta. Bend loss dependence of bend orientations are also studied. Excellent agreement between experiment and simulation is obtained.
An Artificial Aptazyme‐Based Riboswitch and its Cascading System in E. coli (2007)

Flick of the switch. We used an aptazyme and an anti-ribosome binding site (RBS) to construct a novel artificial ribozyme-based riboswitch that functions in E. coli (see figure). This riboswitch worked well at low temperature and activated its own ribozyme and gene expression in the presence of targets even though it is ribozyme based. We also improved the switching efficiency by constructing a cascading system.
Transfection of the human muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, pancl: Integration, expression and ultrastructural changes (1991)

Full-length cDNA for the human mucin Muc 1 gene under the control of the beta actin promoter was transfected into a morphologically poorly differentiated pancreatic tumor cell line, Panc 1, by the DEAE-dextran method. Integration of the foreign Muc 1 cDNA occurred at multiple sites in the genome of Panc 1. Northern blot analysis showed Muc 1 expression in cells transfected with the Muc 1 cDNA, but not in control cells transfected with vector alone or an antisense Muc 1 cDNA construct. Transfection of Panc 1 with Muc 1 cDNA did not cause any detectable alteration or rearrangements in the Muc 1 gene or cDNA. Western blot analysis of cell lysates from the transfected lines using a monoclonal antibody reactive with the Muc 1 protein (HMFG-2) demonstrated that Muc 1 protein expression correlated with the Northern blot data. Immunoperoxidase staining using HMFG-2 showed that Muc 1 protein was expressed in less than 5% of control Panc 1 cells, whereas greater than 95% of cells transfected with Muc 1 cDNA expressed the protein. Ultrastructural examination of Muc 1-transfected cells demonstrated the formation of dense core granules and increased amounts of rough endoplasmic reticulum.
Formation of an Interlocked Quadruplex Dimer by d(GGGT) (2004)

A tetranucleotide sequence d(GGGT) has been shown to self-assemble into an interlocking quadruplex dimer. UV-melting studies indicated the existence of two species that each showed distinct quadruplex melting transitions, a low-T(m) species, Q(l), and a high-T(m) species, Q(h). Conditions were controlled to favor the formation of either Q(l) or Q(h). Q(l) and Q(h) each showed circular dichroism spectra characteristic of parallel quadruplexes. Negative ion nano-electrospray ionization mass spectrometry confirmed that Q(l) was a tetrameric complex, d(GGGT)(4), and Q(h) was an octameric complex, d(GGGT)(8). High-resolution (1)H NMR spectroscopy evidenced that d(GGGT)(4) was a C(4)-symmetric parallel tetramolecular quadruplex. The (1)H NMR spectrum of d(GGGT)(8) was consistent with a structure formed by the dimerization of a parallel, "slipped" tetramolecular quadruplex that has its diagonal strands staggered by one base. This "slippage" results in two guanine bases at the 5' end of the quadruplex being presented diagonally that are not involved in tetrads. Two such "slipped" quadruplexes dimerize via these free G-bases at the 5' ends by forming an extra G-tetrad. Each "slipped" quadruplex contributes two guanine bases to this extra G-tetrad. The formation of a novel GTGT tetrad is also observed at both the 3' ends of the interlocked quadruplex dimer.
Three‐Dimensional Lanthanoid‐Containing Coordination Frameworks: Structure, Magnetic and Fluorescent Properties (2005)

Abstract Two lanthanoid‐containing 3D coordination polymers, [Gd 2 L 3 (H 2 O) 2 ] n ( 1 ) and {[TbL 1.5 (H 2 O)] · 0.5H 2 O} n ( 2 ) (L = succinate), have been prepared by hydrothermal reaction. The difference in structure between the two 3D coordination polymers is a result of the flexibility of the ligand conformation. The magnetic properties of 1 and 2 have been investigated in the 1.8–300 K range. Both complexes exhibit ferromagnetic interaction between lanthanoid ions. AC magnetic measurements revealed long‐range magnetic order in complex 2 . Especially 2 integrates the ferromagnetic, fluorescent and porous properties into a single entity. This motif may be developed to achieve new multifunctional molecular‐based materials. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2005)
Evaluation of the Cellular Origins of Heterotopic Ossification (2014)

Heterotopic ossification (HO), acquired or hereditary, is featured by the formation of bone outside of the normal skeleton. Typical acquired HO is a common, debilitating condition associated with traumatic events. Cardiovascular calcification, an atypical form of acquired HO, is prevalent and associated with high rates of cardiovascular mortality. Hereditary HO syndromes, such as fibrodysplasia ossificans progressiva and progressive osseous heteroplasia, are rare, progressive, life-threatening disorders. The cellular origins of HO remain elusive. Some bona fide contributing cell populations have been found through genetic lineage tracing and other experiments in vivo, and various other candidate populations have been proposed. Nevertheless, because of the difficulties in establishing cellular phenotypes in vivo and other confounding factors, the true identities of these populations are still uncertain. This review critically evaluates the accumulating data in the field. The major focus is on the candidate populations that may give rise to osteochondrogenic lineage cells directly, not the populations that may contribute to HO indirectly. This issue is important not solely because of the clinical implications, but also because it highlights the basic biological processes that govern bone formation.