gebhart/patrepeval huggingface dataset:
Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae. (1995)

A temperature-sensitive mre11-1 mutation of Saccharomyces cerevisiae causes defects in meiotic recombination and DNA repair during vegetative growth at a restrictive temperature. We cloned the MRE11 gene and found that it encodes a 643-amino acid protein with a highly acidic region containing a heptad repeat of Asp at its C-terminus and is located downstream of YMR44 near the RNA1 locus on the right arm of chromosome XIII. Transcripts of the MRE11 gene increased transiently and showed the same kinetics as that of the RAD50 gene during meiosis. In a mre11 disruption mutant (mre11::hisG), meiosis-specific double-strand break (DSB) formation is abolished. A comparison of the properties of mre11::hisG and a rad50 deletion mutant (rad50 delta) indicated that both mutants exhibited similar phenotypes in both meiosis and mitosis. Characterization of two double mutants, mre11::hisG rad50 delta and mre11::hisG rad50S, showed that MRE11 and RAD50 belong to the same epistasis group with respect to meiotic DSB formation and mitotic DNA repair. Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo. These results suggest that Mre11 and Rad50 proteins work in a complex in DSB formation and DNA repair during vegetative growth.
Adiabaticity criterion and the shortest adiabatic mode transformer in a coupled-waveguide system (2009)

By analyzing the propagating behavior of the supermodes in a coupled-waveguide system, we have derived a universal criterion for designing adiabatic mode transformers. The criterion relates epsilon, the fraction of power scattered into the unwanted mode, to waveguide design parameters and gives the shortest possible length of an adiabatic mode transformer, which is approximately 2/piepsilon1/2 times the distance of maximal power transfer between the waveguides. The results from numerical calculations based on a transfer-matrix formalism support this theory very well.
MANF improves the MPP+/MPTP-induced Parkinson's disease via improvement of mitochondrial function and inhibition of oxidative stress. (2018)

MANF can attenuate the neuronal lesion in MPTP/MPP<sup>+</sup>-induced PD mice, which may be related to the improvement of mitochondrial function and inhibition of oxidative stress.
Adaptive cellular interactions in the immune system: the tunable activation threshold and the significance of subthreshold responses. (1992)

A major challenge for immunologists is to explain how the immune system adjusts its responses to the microenvironmental context in which antigens are recognized. We propose that lymphocytes achieve this by tuning and updating their responsiveness to recurrent signals. In particular, cellular anergy in vivo is a dynamic state in which the threshold for a stereotypic mode of activation has been elevated. Anergy is associated with other forms of cellular activity, not paralysis. Cells engaged in such subthreshold interactions mediate functions such as maintenance of immunological memory and control of infections. In such interactions, patterns of signals are recognized and classified and evoke selective responses. The robust mechanism proposed for segregation of suprathreshold and subthreshold immune responses allows lymphocytes to use recognition of self-antigens in executing physiological functions. Autoreactivity is allowed where it is dissociated from uncontrolled aggression.
<title>Investigation of highly scattering media using near-infrared continuous wave tunable semiconductor laser</title> (1995)

The principle of frequency modulated continuous wave radar (FMCW) has been applied to optical frequencies. By tuning the wavelength of a semiconductor laser with time, high resolution imaging in scattering media is possible. The spatial resolution depends mainly on the tuning range of the laser. Several tuning principles to be used in optical FMCW are discussed. As a coherent detection scheme with small bandwidth is used, low noise and high dynamic range are expected. The basic theory is outlined and experimental results are presented. Nonlinear tuning characteristics that degrade the resolution are discussed and interferometric methods enhancing system characteristics are proposed.
Cluster and calendar based visualization of time series data (2003)

A new method is presented to get an insight into univariate time series data. The problem addressed is how to identify patterns and trends on multiple time scales (days, weeks, seasons) simultaneously. The solution presented is to cluster similar daily data patterns, and to visualize the average patterns as graphs and the corresponding days on a calendar. This presentation provides a quick insight into both standard and exceptional patterns. Furthermore, it is well suited to interactive exploration. Two applications, numbers of employees present and energy consumption, are presented.
Autoimmune Thyroiditis Presenting as Palmoplantar Keratoderma (2010)

Palmoplantar keratoderma is a heterogeneous group of hereditary and acquired disorders characterized by abnormal thickening of palms and soles. Hypothyroidism is an unusual cause of palmoplantar keratoderma, rarely reported in the literature. We report a case of a 43-year-old woman presented with a 3-month history of a diffuse palmoplantar hyperkeratosis unresponsive to topical keratolytics and corticosteroids. Her past medical and family histories were unremarkable. She complained of recent asthenia, mood changes and constipation. Laboratory evaluation revealed an autoimmune thyroiditis with hypothyroidism. Other causes of acquired palmoplantar keratoderma were excluded. After hormonal replacement therapy institution, a gradual improvement of skin condition was observed. The diagnosis of underlying causes for acquired palmoplantar keratoderma can be a difficult task; however its recognition is essential for successful treatment results. Although a very rare association, hypothyroidism must be suspected in patients with acquired palmoplantar keratoderma, particularly when it occurs in association with systemic symptoms.
Enhancement of Silicon-Based Inductor Q-Factor Using Polymer Cavity (2012)

A simple low-cost post-complementary metal-oxide-semiconductor-compatible process to enhance the performance of the planar spiral inductor in standard silicon (Si) substrate is demonstrated. In this process, the high loss and high permittivity of Si which reduces the inductor's performance is replaced by a lower loss and lower permittivity thick polymer material. In this way, a measured high Q-factor of more than 37 and a high self-resonance frequency of 25 GHz are achieved for a 1.7-nH inductor. Using this newly developed process, the performance of the inductor can be further improved by optimizing the design.
Hyper-Activated Pro-Inflammatory CD16+ Monocytes Correlate with the Severity of Liver Injury and Fibrosis in Patients with Chronic Hepatitis B (2011)

These distinct properties of monocyte/macrophage subpopulations participate in fostering the inflammatory microenvironment and liver damage in CHB patients and further represent a collaborative scenario among different cell types contributing to the pathogenesis of HBV-induced liver disease.
CARTILAGE RODS AS A POTENTIAL MATERIAL FOR PENILE RECONSTRUCTION (1998)

Chondrocytes seeded on preformed biodegradable polymer structures form cartilage rods. The use of an entirely autologous system composed of biodegradable polymers and chondrocytes precludes an immunological reaction. This technology appears to be useful for the creation of a biocompatible malleable penile prosthesis, which may be useful in children with ambiguous genitalia and patients undergoing penile reconstruction.
Excited States of Nucleotides and Singlet Energy Transfer in Polynucleotides (1967)

We have measured the absorption, fluorescence, and phosphorescence spectra of nucleotides at 77°K. The order of decreasing energies is A, U, T, G, C for the singlet, and U−, C, G, T−, A, T for the triplet. Besides the quantum yields of fluorescence and phosphorescence, we also determined the yield for intersystem crossing to the triplet, using the amplitude of the Δm=2 triplet ESR signal. We find internal conversion occurring from both the singlet and triplet states. From the excitation energies in the neutral and ionized molecules, the stability of the ionizable proton in the excited states can be compared to that in the ground state, and the results are applied to the question of proton transfer from T to A in DNA. From a discussion of the various cases of intermolecular energy transfer for singlets, and considering the broadband spectra of the nucleotides, it is shown that Förster's ``very weak case'' is applicable. We emphasize that two subcases must be distinguished, depending on whether transfer occurs faster than, or slower than, vibrational relaxation. The justification of the approximations in our treatment is given in the Appendix. Assuming dipole—dipole coupling, the rate of transfer is estimated in the nucleotides in the ``before relaxation'' subcase. In the ``after relaxation'' subcase the rate of transfer is calculated using the emission data obtained in this study. In a majority of the pairs of nucleotides, ``after relaxation transfer'' at the Watson—Crick distances is allowed. These considerations are discussed briefly in reference to transfer in polyadenylic acid and DNA.
WHO EXPERT COMMITTEE ON BIOLOGICAL STANDARDIZATION (2007)

This report presents the recommendations of a WHO Expert Committee commissioned to coordinate activities leading to the adoption of international recommendations for the production and control of vaccines and other biologicals and the establishment of international biological reference materials. The report starts with a discussion of general issues brought to the attention of the Committee and provides information on the status and development of reference materials for various antibodies, antigens, blood products and related substances, cytokines, growth factors, and endocrinological substances. The second part of the report, of particular relevance to manufacturers and national regulatory authorities, contains guidelines on quality, safety and effi cacy of live attenuated rotavirus vaccines; DNA vaccines; a biosafety risk assessment for production and quality control of human infl uenza pandemic vaccines; recommendations for inactivated rabies vaccines produced in cell substrates and embryonated eggs; for whole cell pertussis vaccine; and for production, control and regulation of human plasma for fractionation. Also included are a list of recommendations, guidelines and other documents for biological substances used in medicine, and of international standards and reference reagent for biological substances. BIOLOGICAL
Neutrophil Gelatinase-Associated Lipocalin in Patients with Autosomal-Dominant Polycystic Kidney Disease (2007)

It is known that many tubular proteins are involved in the pathogenesis of autosomal-dominant polycystic kidney disease (ADPKD), which causes 8-10% of the cases of end-stage renal disease (ESRD) worldwide. Neutrophil gelatinase-associated lipocalin (NGAL) is a protein expressed on tubular cells of which the production is markedly increased in response to harmful stimuli such as ischemia or toxicity. In the present study, serum and urinary NGAL levels were evaluated in 26 ADPKD subjects. Both levels were significantly higher in patients than in controls (sNGAL 174 +/- 52 vs. 50 +/- 27 ng/ml, p < 0.05; uNGAL 119 +/- 42 vs. 7 +/- 6 ng/ml, p < 0.005) and a close correlation was also found between these parameters and the residual renal function (sNGAL/GFR: r = -0.8, p = 0.006; sNGAL/Creatinine: r = 0.9, p = 0.007; uNGAL/GFR: r = -0.49, p < 0.05; uNGAL/Creatinine: r = 0.84, p < 0.001). Patients were further divided into two groups according to the cystic development assessed with echotomography; subjects with higher cystic growth (HCG) presented higher sNGAL and uNGAL levels with respect to others (sNGAL: 242 +/- 89 vs. 88 +/- 34 ng/ml, p < 0.05; uNGAL: 158 +/- 45 vs. 73 +/- 27 ng/ml, p < 0.05). The strict correlation between NGAL levels and residual renal function is perfectly in accord with recent studies on patients with other ESRD-associated diseases. We can hypothesize that tubular cells produce big quantities of NGAL as a consequence of increased apoptosis following chronic damage or as a compensatory response, similar to that observed in acute stress conditions (ischemia, toxicity ...). Finally, our last finding that patients with HCG showed higher levels of NGAL suggests that this protein could be also involved in the cyst growth process, as previously reported about epithelial and tumoral expansion.
A Novel 16-Kilodalton Cellular Protein Physically Interacts with and Antagonizes the Functional Activity of c- myc Promoter-Binding Protein 1 (2001)

We initially identified c-myc promoter-binding protein 1 (MBP-1) from a human cervical carcinoma cell expression library which negatively regulates c-myc promoter activity. A recent study demonstrated that MBP-1 acts as a general transcriptional repressor (A. K. Ghosh, R. Steele, and R. B. Ray, Mol. Cell. Biol. 19:2880-2886, 1999). In order to identify the cellular protein(s) interacting with MBP-1 for transcriptional regulation, a HeLa cell cDNA expression library was screened using a yeast two-hybrid system. An MBP-1-interacting cDNA encoding a polypeptide of 140 amino acid residues with an approximate molecular mass of 16 kDa was identified and named MBP-1 interacting protein-2A (MIP-2A). MIP-2A has a sequence similarity with an unknown mRNA and SEDL. Mutations in the SEDL gene, located at human chromosome Xp22, has recently been implicated with an X-linked genetic disease, although the function of SEDL gene product was not determined (A. K. Gedeon et al., Nat. Genet. 22:400-404, 1999). However, our results suggested the localization of MIP-2A at human chromosome 19. The specificity of interaction between MBP-1 and MIP-2A was verified by an in vitro glutathione S-transferase pulldown experiment, a mammalian two-hybrid analysis, and in vivo coimmunoprecipitation assays. Further analysis revealed that the amino-terminal domain of MBP-1 (amino acids 1 to 95) interacts with MIP-2A. Immunofluorescent staining suggested colocalization of MIP-2A and MBP-1 primarily in the perinuclear membrane of cells. Functional analysis demonstrated that MIP-2A relieves MBP-1 mediated transcriptional repression on c-myc promoter. Additionally, MIP-2A antagonizes cell growth regulatory role of MBP-1. Taken together, these results suggest the functional interaction of MIP-2A and MBP-1 in cell growth regulation.
Race and Gender Exclusivity in Legal Scholarship (1997)

In 1992 the American Association of Law Schools recommended that new forms of scholarship be given the same consideration for promotion and tenure as "more traditional" scholarship. 1 Traditional scholarship includes vocational, doctrinal, and interdisciplinary writing, 2 and aspires to be objective and analytical, and to cover all relevant arguments.'As the dominant model, doctrinal scholarship is the progeny of Christopher Columbus Langdell'
DNA recognition by DNase I (1994)

Bovine pancreatic DNase I shows a strong preference for double-stranded substrates and cleaves DNA with strongly varying cutting rates suggesting that the enzyme recognises sequence-dependent structural variations of the DNA double helix. The complicated cleavage pattern indicates that several local as well as global helix parameters influence the cutting frequency of DNase I at a given bond. The high resolution crystal structures of two DNase I-DNA complexes showed that the enzyme binds tightly in the minor groove, and to the sugar-phosphate backbones of both strands, and thereby induces a widening of the minor groove and a bending towards the major groove. In agreement with biochemical data this suggests that flexibility and minor groove geometry are major parameters determining the cutting rate of DNase I. Experimental observations showing that the sequence environment of a dinucleotide step strongly affects its cleavage efficiency can be rationalized by the fact that six base pairs are in contact with the enzyme. Mutational analysis based on the structural results has identified critical residues for DNA binding and cleavage and has lead to a proposal for the catalytic mechanism.
Enrichment for STRO‐1 expression enhances the cardiovascular paracrine activity of human bone marrow‐derived mesenchymal cell populations (2010)

Abstract The cardiovascular therapeutic potential of bone marrow mesenchymal stromal/stem cells (MSC) is largely mediated by paracrine effects. Traditional preparation of MSC has involved plastic adherence‐isolation. In contrast, prospective immunoselection aims to improve cell isolation by enriching for mesenchymal precursor cells (MPC) at higher purity. This study compared the biological characteristics and cardiovascular trophic activity of plastic adherence‐isolated MSC (PA‐MSC) and MPC prepared from the same human donors by immunoselection for stromal precursor antigen‐1 (STRO‐1). Compared to PA‐MSC, STRO‐1‐MPC displayed greater (1) clonogenicity, (2) proliferative capacity, (3) multilineage differentiation potential, and (4) mRNA expression of mesenchymal stem cell‐related transcripts. In vitro assays demonstrated that conditioned medium from STRO‐1‐MPC had greater paracrine activity than PA‐MSC, with respect to cardiac cell proliferation and migration and endothelial cell migration and tube formation. In keeping with this, STRO‐1‐MPC exhibited higher gene and protein expression of CXCL12 and HGF. Inhibition of these cytokines attenuated endothelial tube formation and cardiac cell proliferation, respectively. Paracrine responses were enhanced by using supernatant from STRO‐1 Bright MPC and diminished with STRO‐1 Dim conditioned medium. Together, these findings indicate that prospective isolation gives rise to mesenchymal progeny that maintain a higher proportion of immature precursor cells compared to traditional plastic adherence‐isolation. Enrichment for STRO‐1 is also accompanied by increased expression of cardiovascular‐relevant cytokines and enhanced trophic activity. Immunoselection thus provides a strategy for improving the cardiovascular reparative potential of mesenchymal cells. J. Cell. Physiol. 223: 530–540, 2010. © 2010 Wiley‐Liss, Inc.
Conversion of Wood To Carbohydrates (1937)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConversion of Wood To CarbohydratesFriedrich BergiusCite this: Ind. Eng. Chem. 1937, 29, 3, 247–253Publication Date (Print):March 1, 1937Publication History Published online1 May 2002Published inissue 1 March 1937https://pubs.acs.org/doi/10.1021/ie50327a002https://doi.org/10.1021/ie50327a002research-articleACS PublicationsRequest reuse permissionsArticle Views513Altmetric-Citations64LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Voltammetric anion responsive sensors based on modulation of ion permeability through Langmuir-Blodgett films containing synthetic anion receptors (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVoltammetric anion responsive sensors based on modulation of ion permeability through Langmuir-Blodgett films containing synthetic anion receptorsShinobu. Nagase, Masamitsu. Kataoka, Ryuichi. Naganawa, Ryoko. Komatsu, Kazunori. Odashima, and Yoshio. UmezawaCite this: Anal. Chem. 1990, 62, 13, 1252–1259Publication Date (Print):July 1, 1990Publication History Published online1 May 2002Published inissue 1 July 1990https://pubs.acs.org/doi/10.1021/ac00212a011https://doi.org/10.1021/ac00212a011research-articleACS PublicationsRequest reuse permissionsArticle Views189Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Synthesis and Optical Properties of Lead-Free Cesium Tin Halide Perovskite Nanocrystals (2016)

Metal halide perovskite crystal structures have emerged as a class of optoelectronic materials, which combine the ease of solution processability with excellent optical absorption and emission qualities. Restricting the physical dimensions of the perovskite crystallites to a few nanometers can also unlock spatial confinement effects, which allow large spectral tunability and high luminescence quantum yields at low excitation densities. However, the most promising perovskite structures rely on lead as a cationic species, thereby hindering commercial application. The replacement of lead with nontoxic alternatives such as tin has been demonstrated in bulk films, but not in spatially confined nanocrystals. Here, we synthesize CsSnX3 (X = Cl, Cl0.5Br0.5, Br, Br0.5I0.5, I) perovskite nanocrystals and provide evidence of their spectral tunability through both quantum confinement effects and control of the anionic composition. We show that luminescence from Sn-based perovskite nanocrystals occurs on pico- to nanosecond time scales via two spectrally distinct radiative decay processes, which we assign to band-to-band emission and radiative recombination at shallow intrinsic defect sites.