gebhart/patrepeval huggingface dataset:
Impact of Subcortical Ischemic Lesions on Behavior and Cognition (2002)

Both cerebrovascular disease and AD are common in the elderly. There are various types of underlying damage to tissue and vessels in vascular cognitive impairment, and the neural mechanisms producing cognitive impairment and the clinical picture are different among the subtypes. Among them, small subcortical infarcts disrupting cortico-subcortical circuits and white matter lesions are the commonest types, and the combination of these two types of lesions might be more realistic than each pure form. Moreover, cognitive impairment of vascular origin may be superimposed on AD. Both vascular and degenerative mechanisms contribute to the development of cognitive impairment, especially in old age, whether they are two independent parallel processes or interacting pathologies. Subcortical small lesions involving the thalamus, caudate, and globus pallidus disrupt cortico-subcortical circuits, resulting in cognitive dysfunction. Disruption of the frontal-subcortical circuits leads to cognitive impairment with striking frontal lobe features, and disruption of the memory-related circuits leads to amnesia. White matter changes, which are certainly related to chronic cerebral ischemia in some patients, are another issue. Patients with dementia and white matter changes may have either AD with cerebrovascular changes or a form of VaD, or a combination of these two etiologies. However, our series of studies have suggested that white matter changes in AD patients are superimposed phenomena of vascular origin and that white matter changes contribute to specific neurological and neuropsychiatric manifestations, but not to global cognitive impairment, which is more closely associated with the degenerative process.
The continuously fed batch reactor for measuring microbial growth rates (1983)

The contiuously fed batch reactor (CFBR) is proposed as an alternative technique to the traditional chemostat and batch cultures, for measuring microbial growth rates. After reviewing the pitfalls which plague the conventional growth measurement techniques, the methodology for operating the CFBR to generate specific growth-rate-versus-substrate-concentration data is detailed. This information is extracted from the transient state of the CFBR where both the biomass and substrate concentration show extrema in time. It is suggested that the CFBR can be used for measuring microbial growth rates at low rates at low substrate concentrations where the chemostat method normally encounters difficulties.
Tripyranoside precursors for ansamycins. Pyranosidic homologation. 6 (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTripyranoside precursors for ansamycins. Pyranosidic homologation. 6Bert Fraser-Reid, Bruce F. Molino, Leon Magdzinski, and David R. MootooCite this: J. Org. Chem. 1987, 52, 20, 4505–4511Publication Date (Print):October 1, 1987Publication History Published online1 May 2002Published inissue 1 October 1987https://pubs.acs.org/doi/10.1021/jo00229a014https://doi.org/10.1021/jo00229a014research-articleACS PublicationsRequest reuse permissionsArticle Views547Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A novel synthesis of dihydropyrimidines (1987)

P. J. Garratt, C. J. Hobbs, C. S. J. Walpole and R. Wrigglesworth, J. Chem. Soc., Chem. Commun., 1987, 568 DOI: 10.1039/C39870000568
Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases (2002)

Guanine (G)-rich oligodeoxyribonucleotides (ODNs) can form undesired complexes by self association through non-Watson-Crick interactions. These aggregates can compromise performance of DNA probes and make genetic analysis unpredictable. We found that the 8-aza-7-deazaguanine (PPG), a pyrazolo[3,4-d]pyrimidine analog, reduces guanine self association of G-rich ODNs. In the PPG heterocycle, the N-7 and C-8 atoms of G are interposed. This leaves the ring system with an electron density similar to G, but prevents Hoogsteen-bonding associated with N-7. ODNs containing multiple PPG bases were easily prepared using a dimethylformamidine-protected phosphoramidite reagent. Substitution of PPG for G in ODNs allowed formation of more stable DNA duplexes. When one or more PPGs were substituted for G in ODNs containing four or more consecutive Gs, G aggregation was eliminated. Substitution of PPG for G also improved discrimination of G/A, G/G and G/T mismatches in Watson-Crick hybrids. Use of PPG in fluorogenic minor groove binder probes was also explored. PPG prevented aggregation in MGB probes (MGB(TM) is a trademark of Epoch Biosciences) and allowed use of G-rich sequences. An increased signal was observed in 5'-PPG probes due to reduced quenching of fluorescein by PPG. In summary, substitution of PPG for G enhances affinity, specificity, sensitivity and predictability of G-rich DNA probes.
Amniotic Membrane Transplantation for Symptomatic Conjunctivochalasis Refractory to Medical Treatments (2000)

Amniotic membrane transplantation can be considered as an effective means for conjunctival surface reconstruction during removal of conjunctivochalasis.
AlGaN/GaN high electron mobility transistors on Si(111) substrates (2001)

AlGaN/GaN high electron mobility transistors (HEMTs) on silicon substrates have for the first time been realized using organometallic vapor phase epitaxy (OMVPE). Using 1 /spl Omega/-cm p-Si(111), these devices exhibited static output characteristics with low output conductance and isolation approaching 80 V. Under microwave rf operation, the substrate charge becomes capacitively coupled and parasitically loads these devices thereby limiting their performance. As a result, typical 0.3 /spl mu/m gate length devices show a 25 GHz cutoff frequency, with near unity f/sub max//f/sub T/ ratio and 0.55 W/mm output power. A small-signal equivalent circuit incorporating elements representing the parasitic substrate loading accurately models the measured S-parameters. Removal of the conductive substrate is one way to effectively eliminate this parasitic loading. Through backside processing, freestanding 0.4-mm HEMT membranes with no thermal management were demonstrated and exhibited a significant improvement in their f/sub max//f/sub T/ ratio up to 2.5 at the cost of lower f/sub T/ and f/sub max/ along with an almost four-fold reduction of I/sub dss/.
Novel signatures of cancer‐associated fibroblasts (2013)

Increasing evidence indicates the importance of the tumor microenvironment, in particular cancer-associated fibroblasts, in cancer development and progression. In our study, we developed a novel, visually based method to identify new immunohistochemical signatures of these fibroblasts. The method employed a protein list based on 759 protein products of genes identified by RNA profiling from our previous study, comparing fibroblasts with differential growth-modulating effect on human cancers cells, and their first neighbors in the human protein interactome. These 2,654 proteins were analyzed in the Human Protein Atlas online database by comparing their immunohistochemical expression patterns in normal versus tumor-associated fibroblasts. Twelve new proteins differentially expressed in cancer-associated fibroblasts were identified (DLG1, BHLHE40, ROCK2, RAB31, AZI2, PKM2, ARHGAP31, ARHGAP26, ITCH, EGLN1, RNF19A and PLOD2), four of them can be connected to the Rho kinase signaling pathway. They were further analyzed in several additional tumor stromata and revealed that the majority showed congruence among the different tumors. Many of them were also positive in normal myofibroblast-like cells. The new signatures can be useful in immunohistochemical analysis of different tumor stromata and may also give us an insight into the pathways activated in them in their true in vivo context. The method itself could be used for other similar analysis to identify proteins expressed in other cell types in tumors and their surrounding microenvironment.
Enhancement of the intracranial arterial wall at MR imaging: relationship to cerebral atherosclerosis. (1995)

Arterial wall enhancement is related to aging and is probably due to neovascularity in association with atherosclerotic plaques. This finding may permit assessment of intracranial atherosclerosis and other vascular diseases.
Methanogens in Human Health and Disease (2012)

There is growing evidence that host/microbial interactions within the gut can have a profound impact on human health and disease; in fact, the intestinal microflora have been shown to influence the innate physiology, biochemistry, immunology, maturation of the vasculature, and gene expression in a host. Although most research has focused on gut bacteria, current evidence suggests that the Archaea—an ancient domain of single-celled organisms—are resident within the gut in high numbers, and have direct and indirect effects on the host. In particular, the methanogens are an essential component of luminal intestinal microbial ecosystems. Methanogens oxidize hydrogen to produce methane and ensure more complete fermentation of carbohydrate substrates, leading to higher production and adsorption of short-chain fatty acids, which may lead to obesity. Methane, the key product of carbohydrate fermentation by the methanogens, has long been thought to produce no ill effects in humans aside from gaseous distention. However, recent evidence has linked methane production to the pathogenesis of constipation and irritable bowel syndrome (IBS), as well as obesity. In particular, a significant percentage of patients with IBS and constipation excrete methane, suggesting an overabundance of methanogenic archaea in their gut. Methane by itself may influence intestinal transit and pH and facilitate development of constipation. If methane has a direct or indirect effect on intestinal transit, attempting to manipulate methanogenic flora may serve as a novel therapeutic option. Thus, understanding methanogens and their role in gut function/dysfunction is vital to our understanding of human health and disease.
Depletion of Methionine Aminopeptidase 2 Does Not Alter Cell Response to Fumagillin or Bengamides (2004)

Inhibition of endothelial cell growth by fumagillin has been assumed to be mediated by inhibition of the molecular target methionine aminopeptidase 2 (MetAp2). New data show that depletion of MetAp2 by siRNA does not inhibit endothelial cell growth. Moreover, MetAp2-depleted endothelial cells remain responsive to inhibition by either fumagillin or a newly identified MetAp2 enzyme inhibitor. These data suggest that MetAp2 function is not required for endothelial cell proliferation.
Magnetooptic properties and applications of bismuth substituted iron garnets (1976)

Using the results of the phenomenological and microscopic description of magnetooptically active transitions presented in the beginning of the article, a review is made of the progress achieved in the understanding of the origin of the large bismuth induced magnetooptic effects in iron garnets. For applications involving the Faraday configuration, optical efficiencies are determined by the ratio of Faraday rotation (θ) to optical absorption (α). The dependence of θ/α on such variables as bismuth concentration, <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4\piM_{s}</tex> and impurity ions is discussed with its spectral behavior. Finally, the behavior of bismuth substituted iron garnets in devices previously devised using unsubstituted iron garnets is discussed, in conjunction with devices which have only become feasible as a consequence of improvements in the optical properties of such iron garnets.
High-level expression of the tick-borne encephalitis virus NS1 protein by using an adenovirus-based vector: protection elicited in a murine model (1992)

Tick-borne encephalitis virus (TBEV) encodes an abundant, highly immunogenic nonstructural glycoprotein, NS1. The function of this protein has yet to be determined. We have cloned the NS1 gene from the Neudorfl strain of TBEV under the control of the powerful constitutive cytomegalovirus major immediate-early promoter into an adenovirus E1 deletion mutant. The novel combination of the cytomegalovirus immediate-early promoter and the adenovirus vector produced extremely high levels of NS1 expression in cells which do not support replication of the adenovirus deletion mutant. The recombinant protein was shown to be indistinguishable from authentic TBEV NS1 in its (i) apparent molecular weight by polyacrylamide gel electrophoresis, (ii) glycosylation pattern, (iii) ability to form high-molecular-weight complexes, and (iv) ability to be secreted from cells. Appropriate processing of NS1 expressed by the adenovirus recombinant occurred independently of any additional TBEV-encoded gene function. When directly inoculated into mice, the recombinant adenovirus RAd51 was shown to elicit an antibody response to the TBEV NS1 protein. Immunization with RAd51 conferred protection against challenge with TBEV.
Effect of Meso-Substituents on the Osmium Tetraoxide Reaction and Pinacol−Pinacolone Rearrangement of the Corresponding vic-Dihydroxyporphyrins (2001)

To investigate the effects of electron-donating and electron-withdrawing substituents upon the reaction of porphyrins with osmium tetraoxide, and the pinacol-pinacolone rearrangement of the resulting diols, a series of meso-substituted porphyrins were prepared by total synthesis. Porphyrins with electron-donating substitutents at the meso-positions gave vic-dihydroxychlorins in which the adjacent pyrrole subunit was predominantly oxidized. No such selectivity was observed in a porphyrin containing a methoxycarbonyl as the electron-withdrawing group, whereas a formyl substituent again resulted in oxidation at the pyrrole unit adjacent to the meso-substituent. Under pinacol-pinacolone conditions, vic-dihydroxy chlorins containing 4-methoxyphenyl or 3,5-dimethoxyphenyl groups at the meso-position showed preferential migration of the ethyl group over the methyl group to give 8-ketochlorins, whereas the diol with an n-heptyl substituent under similar reaction conditions gave both 7- and 8-ketochlorins. In contrast, the diol containing a meso-formyl substituent produced the corresponding 7-ketochlorin exclusively. These results indicate that it is not possible to predict the reactivity of meso-substituted porphyrins in the osmium tetraoxide reaction nor the general substituent migratory aptitudes in the pinacol-pinacolone rearrangement based on simple electronic arguments, most likely because many parameters (e.g., meso-beta-pyrrolic steric crowding and long-range electronic effects) ultimately determine the reactivity. The structural assignments of the porphyrin diols and the keto-analogues were confirmed by extensive (1)H NMR studies; some of the dihydroxychlorins and ketochlorins were found to display unusual features in their (1)H NMR spectra.
Vaccine design : the subunit and adjuvant approach (1995)

Provides descriptions of scientific theories, product development strategies, and results from preclinical studies for many of the newest subunit vaccines or components designed for use in subunit vaccine formulation, such as adjuvants and vaccine delivery systems. The topics highlight many promisin
Mycoplasma pneumoniae attachment: competitive inhibition by mycoplasmal binding component and by sialic acid-containing glycoconjugates (1982)

Attachment of Mycoplasma pneumoniae to human WiDr cell culture monolayers was examined by using radiolabeled M. pneumoniae. The amount of attachment was proportional to the density of the WiDr cells and to the concentration of M. pneumoniae in the assay. Saturation of the monolayers was achieved with 40 micrograms of virulent strain M129 per assay, whereas binding of avirulent strain B176 was 70% less than that of strain M129. A competitive attachment inhibition assay was used to measure specific binding component activity. Attachment was inhibited when WiDr cells were pretreated with unlabeled virulent strain M129, whereas avirulent noncytadsorbing strain B176 did not inhibit attachment as well as the virulent strain. A protein-rich extract prepared from virulent, cytadsorbing strains of M. pneumoniae also inhibited attachment. The amount of inhibition was dependent on the amount of extract used, and units for binding component activity in the extract were calculated from the competitive attachment inhibition assays. The competitive attachment inhibition assay was also used to investigate the nature of the receptor site on the WiDr cells. Attachment was inhibited when the radiolabeled M. pneumoniae suspensions were pretreated with human sialoglycoproteins, such as orosomucoid and ceruloplasmin, and bovine gangliosides. These findings support the present concept that the mammalian receptor site for M. pneumoniae is a sialic acid-containing glycoprotein.
Engineering Escherichia coli BL21(DE3) Derivative Strains To Minimize E. coli Protein Contamination after Purification by Immobilized Metal Affinity Chromatography (2011)

Recombinant His-tagged proteins expressed in Escherichia coli and purified by immobilized metal affinity chromatography (IMAC) are commonly coeluted with native E. coli proteins, especially if the recombinant protein is expressed at a low level. The E. coli contaminants display high affinity to divalent nickel or cobalt ions, mainly due to the presence of clustered histidine residues or biologically relevant metal binding sites. To improve the final purity of expressed His-tagged protein, we engineered E. coli BL21(DE3) expression strains in which the most recurring contaminants are either expressed with an alternative tag or mutated to decrease their affinity to divalent cations. The current study presents the design, engineering, and characterization of two E. coli BL21(DE3) derivatives, NiCo21(DE3) and NiCo22(DE3), which express the endogenous proteins SlyD, Can, ArnA, and (optionally) AceE fused at their C terminus to a chitin binding domain (CBD) and the protein GlmS, with six surface histidines replaced by alanines. We show that each E. coli CBD-tagged protein remains active and can be efficiently eliminated from an IMAC elution fraction using a chitin column flowthrough step, while the modification of GlmS results in loss of affinity for nickel-containing resin. The "NiCo" strains uniquely complement existing methods for improving the purity of recombinant His-tagged protein.
Monoclonal Antibodies Specific to the Acute Lymphoblastic Leukemia t(1; 19)-Associated E2A/pbx1 Chimeric Protein: Characterization and Diagnostic Utility (1997)

Nonrandom chromosomal abnormalities are found in most human malignancies, particularly leukemias and lymphomas. A characteristic t(1;19) (q23;p13.3) chromosomal translocation is detected in 5% of childhood acute lymphoblastic leukemia (ALL) cases. This translocation results in the formation of a fusion gene, which leads to the expression of an oncogenic E2A/pbx1 protein. Breakpoints in the E2A gene almost invariably occur within a single intron, and the identical portion of PBX1 is joined consistently to exon 13 of E2A in fusion mRNA. In this article, we report the development of monoclonal antibodies against E2A/pbx1 fusion protein using a specific peptide that corresponds to the junction region of the protein. The obtained antibodies recognize specifically the chimeric E2A/pbx1 fusion protein and lack cross-reactivities with E2A and pbx1. Immunohistochemical staining and flow cytometric studies show that these antibodies can distinguish t(1;19)-positive from t(1;19)-negative leukemic cells. These results indicate that the obtained E2A/pbx1-specific monoclonal antibodies might prove to be valuable diagnostic reagents and important tools for elucidating the mechanisms involved in oncogenesis and progression of t(1;19)-positive childhood ALL.
The Sensitization Phase of T‐Cell‐Mediated Immunitya (1988)

Many cell-mediated immune responses appear to develop in two phases: A sensitization phase in which unprimed or memory T cells interact with dendritic cells to become active lymphoblasts, and an effector phase in which the T lymphoblasts and other presenting cells interact to eliminate the antigen. Antigen presentation is essential to both phases. Here we review several features that are pertinent to the special sensitization role of dendritic cells. First, dendritic cells from lymphoid tissues, blood, and lymph (lymphoid dendritic cells) express very high levels of class I and II MHC products, and these levels cannot be increased by exposure to cytokines such as immune interferon. Second, dendritic cells efficiently cluster antigen-specific T cells during primary responses. Other presenting cells, like macrophages and B lymphocytes, do not form clusters but do bind to sensitized T lymphoblasts. Dendritic-T-cell binding is not inhibited by mAb to CD4 and LFA-1 antigens. It is suggested that a dendritic-cell-specific molecule is required. Third, it is not yet clear if dendritic cells make a "lymphocyte activating factor." However, IL-1 is not produced, even when dendritic cells are in contact with responding T cells. Fourth, dendritic cells have the capacity to migrate from the tissues and move to T-dependent areas. Epidermal Langerhans cells represent a reservoir of tissue dendritic cells but seem to be immunologically immature. The viability and accessory function of the Langerhans cell greatly depend on a single cytokine, granulocyte-macrophage colony stimulating factor (GM-CSF), leading to the proposal that GM-CSF is critical in mobilizing active dendritic cells at the onset of a cell-mediated immune response.