gebhart/patrepeval huggingface dataset:
Protective CD8+ T cell responses against the pre-erythrocytic stages of malaria parasites: an overview (2001)

CD8+ T cells have been implicated as critical effector cells in protection against the pre-erythrocytic stage of malaria in mice and humans following irradiated sporozoite immunization. Immunization experiments in animal models by several investigators have suggested different strategies for vaccination against malaria and many of the targets from liver stage malaria antigens have been shown to be immunogenic and to protect mice from the sporozoite challenge. Several prime/boost protocols with replicating vectors, such as vaccinia/influenza, with non-replicating vectors, such as recombinant particles derived from yeast transposon (Ty-particles) and modified vaccinia virus Ankara, and DNA, significantly enhanced CD8+ T cell immunogenicity and also the protective efficacy against the circumsporosoite protein of Plasmodium berghei and P. yeti. Based on these experimental results the development of a CD8+ T cell inducing vaccine has moved forward from epitope identification to planning stages of safety and immunogenicity trials of candidate vaccines.
Histocompatible Embryonic Stem Cells by Parthenogenesis (2006)

Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.
Germanium/carbon core–sheath nanostructures (2000)

Germanium/carbon core–sheath nanostructures and junctions are produced when Ge nanowires are subject to a thermal treatment in an organic vapor doped vacuum. The organic molecules pyrolyze on the surface of the Ge nanowires and form a continuous graphitic coating. The carbon-sheathed Ge nanowires undergo melting and evaporation at high temperature, which results in the formation of germanium/carbon junctions. These core–sheath nanostructures and junctions generally have diameters of 5–100 nm, sheath thickness of 1–5 nm, and lengths up to several micrometers. This process may prove to be an effective approach to prevent the nanowire surface oxidation and create nanowires with chemically inert surface.
Optimizing Preparation Conditions for Heat‐denatured Whey Protein Solutions to be Used as Cold‐gelling Ingredients (2000)

ABSTRACT: Heat‐denatured whey protein solutions are used to make ingredients that gel at low temperatures. This study examines the influence of holding temperature (65 to 90°C), holding time (5 to 30 min), protein concentration (2 to 12 wt%) and pH (3 to 8) on the rheology and appearance of heat‐denatured whey protein solutions. The optimum preparation conditions required to produce non‐gelled transparent solutions of heat‐denatured proteins were established. The rate of cold‐gelation after the addition of 200 mM NaCl to the heat‐denatured whey protein solutions increased as their initial viscosity increased. It was possible to produce gels with different cold‐gelling characteristics by altering the thermal preparation conditions.
Papain Catalyzed Oligomerization of α‐Amino Acids. Synthesis and characterization of water‐insoluble oligomers of L‐methionine (1980)

Abstract Water‐insoluble oligomers were synthesized from L ‐methionine ethyl ester with papain as the catalyst. L ‐Oligomethionine was obtained in yields of 50% when synthesis was carried out in highly concentrated citrate buffer at pH 5.5. Yields of up to 85% were obtained when the enzymatic synthesis proceeded in distilled water at pH 6.5, the pH being strictly maintained. The insoluble polymer was converted to highly water‐soluble sulfoxide and sulfone derivatives, which consist mainly of an octamer with low amounts of heptamer or hexamer. Most of the carboxyl terminals still contained the ethyl ester group, only a minor part being present in the free acid form. The potential of the enzymatic approach for the synthesis of optically pure and monodisperse oligomers of α‐amino acids is discussed.
Designing a functional type 2 copper center that has nitrite reductase activity within α-helical coiled coils (2012)

One of the ultimate objectives of de novo protein design is to realize systems capable of catalyzing redox reactions on substrates. This goal is challenging as redox-active proteins require design considerations for both the reduced and oxidized states of the protein. In this paper, we describe the spectroscopic characterization and catalytic activity of a de novo designed metallopeptide Cu(I/II)(TRIL23H)(3)(+/2+), where Cu(I/II) is embeded in α-helical coiled coils, as a model for the Cu(T2) center of copper nitrite reductase. In Cu(I/II)(TRIL23H)(3)(+/2+), Cu(I) is coordinated to three histidines, as indicated by X-ray absorption data, and Cu(II) to three histidines and one or two water molecules. Both ions are bound in the interior of the three-stranded coiled coils with affinities that range from nano- to micromolar [Cu(II)], and picomolar [Cu(I)]. The Cu(His)(3) active site is characterized in both oxidation states, revealing similarities to the Cu(T2) site in the natural enzyme. The species Cu(II)(TRIL23H)(3)(2+) in aqueous solution can be reduced to Cu(I)(TRIL23H)(3)(+) using ascorbate, and reoxidized by nitrite with production of nitric oxide. At pH 5.8, with an excess of both the reductant (ascorbate) and the substrate (nitrite), the copper peptide Cu(II)(TRIL23H)(3)(2+) acts as a catalyst for the reduction of nitrite with at least five turnovers and no loss of catalytic efficiency after 3.7 h. The catalytic activity, which is first order in the concentration of the peptide, also shows a pH dependence that is described and discussed.
Clinically significant drug interactions (1999)

Ironically, pharmacotherapy can harm the very patients it is intended to help if clinically significant drug interactions occur. However, knowledge of the role of the hepatic cytochrome P-450 isoenzyme system in drug interactions has expanded greatly with advances in gene-mapping technologies, and an understanding of the system can aid physicians in preventing or minimizing complications from drug interactions mediated through that system. The authors summarize the role of important isoenzymes and drugs that induce and inhibit the P-450 system. They also discuss serious drug interactions mediated through the P-450 system and ways to predict and avoid them. As a special aid, they provide a reference guide which, when used faithfully in prescribing, should result in better drug therapy.
Oral immunization and protection of raccoons (Procyon lotor) with a vaccinia-rabies glycoprotein recombinant virus vaccine. (1986)

Animal rabies control has been frustrated by the existence of multiple wildlife reservoirs and the lack of efficacious oral vaccines. In this investigation, raccoons fed a vaccinia-rabies glycoprotein recombinant virus in a sponge bait developed rabies virus-neutralizing antibody (0.6-54.0 units) and resisted street rabies virus infection 28 and 205 days after feeding. Additional raccoons immunized by oral infusion with attenuated antigenic variants of rabies virus strains CVS-11 and ERA failed to develop rabies virus-neutralizing antibody. This work demonstrates the feasibility of a recombinant virus vaccine containing the rabies glycoprotein gene for immunization of raccoons, and possibly other wildlife, to obtain long-term protection against rabies.
Model-based image coding advanced video coding techniques for very low bit-rate applications (1995)

The paper gives an overview of model-based approaches applied to image coding, by looking at image source models. In these model-based schemes, which are different from the various conventional waveform coding methods, the 3-D properties of the scenes are taken into consideration. They can achieve very low bit rate image transmission. The 2-D model and 3-D model based approaches are explained. Among them, a 3-D model based method using a 3-D facial model and a 2-D model based method utilizing 2-D deformable triangular patches are described. Works related to 3-D model-based coding of facial images and some of the remaining problems are also described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Self-Organization of Organic Liquids on Patterned Self-Assembled Monolayers of Alkanethiolates on Gold (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-Organization of Organic Liquids on Patterned Self-Assembled Monolayers of Alkanethiolates on GoldHans A. Biebuyck and George M. WhitesidesCite this: Langmuir 1994, 10, 8, 2790–2793Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/la00020a047https://doi.org/10.1021/la00020a047research-articleACS PublicationsRequest reuse permissionsArticle Views930Altmetric-Citations126LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Expression of 65- and 67-kilodalton heat-regulated proteins and a 70-kilodalton heat shock cognate protein of Leishmania donovani in macrophages (1993)

Heat shock protein (HSP) expression was examined in murine bone marrow-derived macrophages infected with stationary-phase promastigotes of Leishmania donovani. Immunoblotting performed with a rabbit polyclonal antiserum raised against HSP60 from Heliothis virescens (moth) revealed the de novo appearance of 65- and 67-kDa proteins in leishmania-infected macrophages. A third protein of 60 kDa, which represented murine HSP60, was also detected, and its expression did not change in response to infection. In contrast, expression of the novel 65- and 67-kDa proteins in infected cells was coordinately regulated and, at 24 h of infection, reached maximal levels of 52 to 100% increases above initial levels determined at 3 h. Proteins which had identical electrophoretic mobilities and were similarly regulated in response to heat were also detected in promastigotes. The appearance of these proteins in macrophages was specific to leishmania infection in that neither protein was detected in noninfected cells either in the basal state or following several treatments, including (i) infection with Yersinia pseudotuberculosis, (ii) phagocytosis of Staphylococcus aureus, (iii) NaAsO2 treatment, and (iv) heat shock. Expression of the 65- and 67-kDa heat-regulated Leishmania proteins was also observed to be selective, in that as their concentration was increasing, the abundance of the Leishmania surface protease gp63 in infected cells was noted to decrease. Murine HSP60 but not the Leishmania heat-regulated proteins was also recognized by a distinct rabbit antiserum raised against human HSP60, suggesting the presence of specific determinants within these Leishmania proteins. A monoclonal antibody that recognizes both mammalian HSP70 and HSP70 from plasmodia detected single isoforms of both Leishmania and murine HSP70 in infected cells, and the level of neither protein changed during infection. Moreover, although a murine HSP of 73 kDa was induced in response to both heat shock and NaAsO2 treatment, it was not induced to detectable levels by infection. The rapid and relatively high level of expression of inducible HSP60-related proteins of L. donovani and Leishmania HSP70 in infected macrophages suggests that these proteins are involved in pathogenesis and may be important targets of the immune response.
Epigenetic Reprogramming of HOXC10 in Endocrine-Resistant Breast Cancer (2014)

Resistance to aromatase inhibitors (AIs) is a major clinical problem in the treatment of estrogen receptor (ER)-positive breast cancer. In two breast cancer cell line models of AI resistance, we identified widespread DNA hyper- and hypomethylation, with enrichment for promoter hypermethylation of developmental genes. For the homeobox gene HOXC10, methylation occurred in a CpG shore, which overlapped with a functional ER binding site, causing repression of HOXC10 expression. Although short-term blockade of ER signaling caused relief of HOXC10 repression in both cell lines and breast tumors, it also resulted in concurrent recruitment of EZH2 and increased H3K27me3, ultimately transitioning to increased DNA methylation and silencing of HOXC10. Reduced HOXC10 in vitro and in xenografts resulted in decreased apoptosis and caused antiestrogen resistance. Supporting this, we used paired primary and metastatic breast cancer specimens to show that HOXC10 was reduced in tumors that recurred during AI treatment. We propose a model in which estrogen represses apoptotic and growth-inhibitory genes such as HOXC10, contributing to tumor survival, whereas AIs induce these genes to cause apoptosis and therapeutic benefit, but long-term AI treatment results in permanent repression of these genes via methylation and confers resistance. Therapies aimed at inhibiting AI-induced histone and DNA methylation may be beneficial in blocking or delaying AI resistance.
Thin-Layer Chromatography and Electrospray Mass Spectrometry Coupled Using a Surface Sampling Probe (2002)

A combined surface sampling probe/electrospray emitter was used for the direct readout of thin-layer chromatography plates by electrospray mass spectrometry. The technique was demonstrated with reversed-phase C18 plates using a three-dye mixture composed of methylene blue, crystal violet, and rhodamine 6G for positive ion mode detection and a separate dye mixture containing fluorescein, naphthol blue black, and fast green FCF for negative ion mode detection. Acquisition of mass spectra of components of individual bands on the plate was shown by manual stepping to and sampling from specific locations within the bands. Computer-controlled scanning of development lanes on the plate was illustrated by using multiple ion monitoring in both positive and negative ion modes. Commercial TLC plates were used and no post-separation processing other than drying of the plates was required prior to mass spectrometric analysis. Readout resolution, the limits of scan speed, detection levels, TLC phase, and eluting solvents were investigated and discussed.
Rabbit Monoclonal Antibodies (2005)

Rabbit monoclonal antibodies (RabMAbs) represent a novel category of immunoreagents that may combine the best properties of both mouse monoclonal antibodies (MMAs) and rabbit antisera. In the attempt to verify the performance of this new class of antibodies on paraffin-embedded tissue, RabMAbs against estrogen receptor, progesterone receptor, Ki-67, cyclin D1, CD3, CD5, CD23, and synaptophysin were tested on several tumor types as well as normal tissues. The results were compared with those obtained with classic MMAs against the same antigens. RabMAbs appear to offer increased sensitivity with no apparent loss of specificity. On routine use they permit higher working dilutions (5 to 10 times on average), allowing significant improvement in terms of laboratory efficiency. The robustness of RabMAbs is further proved by the fact that in some instances optimal staining can be obtained even without antigen retrieval. In consideration of the high performance observed, routine use of RabMAbs may contribute significantly to standardize diagnostic immunohistochemical procedures.
Ultrasound Sonication Effects on Silk Fibroin Protein (2013)

Abstract The utilization of silk fibroin protein (SFP) in the field of biomaterials and tissue engineering plays an important role due to its promising mechanical, biological, and processing properties. In the present study, SFP solutions were exposed to ultrasound sonication to assess impact on molecular weight (Mw), sol–gel transition behavior, and physico‐chemical properties. The Mw of SFP was significantly reduced with sonication, determined by sodium dodecyl sulfate polyacrylamide gels (SDS–PAGE) gel electrophoresis. Sol–gel transitions were observed as SFP assembled to form a reversible SFP gel network at different concentrations and sonication time with the formation of a β‐sheet‐rich conformation in the gel state. The conformational changes and morphological properties were correlated over a range of SFP concentrations by circular dichroism (CD), UV spectroscopy, swelling and attenuated total reflectance fourier transformed infrared spectroscopy (ATR‐FTIR). magnified image
Granulocyte activation via a binding site near the C-terminal region of complement receptor type 3 alpha-chain (CD11b) potentially involved in intramembrane complex formation with glycosylphosphatidylinositol-anchored Fc gamma RIIIB (CD16) molecules. (1995)

We report that engagement of a particular epitope near the C-terminal region of complement receptor type 3 (CR3, CD11b/CD18) alpha-chain with CD11b mAb VIM12 induces granulocyte activation with a rise in cytosolic-free Ca2+, actin polymerization, an up-regulation of CR3 cell surface expression and enhanced adhesiveness. Induction of enhanced adhesiveness and homotypic aggregation of human granulocytes represents an active process. It is temperature and energy dependent, requires divalent cations, and an intact cytoskeleton. The mAb VIM12-induced enhanced adhesiveness seems to be mediated, at least in part, by activated CR3 molecules. It can be significantly inhibited, although not completely abolished, with blocking mAbs against adhesiotopes of CR3. VIM12-induced adhesion could be blocked with the serine/threonine inhibitors okadaic acid and calyculin A and with dibuturyl-cAMP but not with the protein kinase inhibitors herbimycin A and staurosporine. We further present evidence that the particular molecular region of CR3 recognized by mAb VIM12 might be involved in the reported intramembrane sugar-lectin type interaction and complex formation between transmembrane CR3 and glycosylphosphatidylinositol (GPI)-anchored Fc gamma RIIIB (CD16) molecules on human granulocytes. Binding of mAb VIM12 to CR3 on granulocytes enhances the release of GPI-anchored Fc gamma RIIIB molecules from granulocytes upon phosphoinositol-phospholipase C treatment. The sugar preparation N-acetyl-D-glucosamine, previously shown to dissociate CR3-Fc gamma RIIIB complex formation, inhibits mAb VIM12 binding. Engagement of CR3 with mAb VIM12 may thus mimic a biologically relevant intramembrane cooperation between two distinct receptor molecules on human granulocytes.
One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.) (2014)

Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience’s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice. Validation was carried out by determining the analytical performance of the new method and comparing it with conventional allele-specific PCR, when genotyping sets of cultivated and weedy rice samples. The conventional technique had a specificity of 0.97 and a sensibility of 0.95, whereas for the KASP method, both parameters were 1.00. The new method has equal accuracy while being more informative and saving time and resources compared with conventional methods, which make it suitable for monitoring imidazolinone-resistant weedy rice in Clearfield rice fields.
Characterization of Variable-Region Genes and Shared Crossreactive Idiotypes of Antibodies Specific for Antigens of Various Influenza Viruses (1987)

Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H3N2) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H3 and H1). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (VK)21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (VH) J558 or VH 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain.
Enhancement of band edge luminescence in ZnSe nanowires (2006)

In order to realize the full potential of nanowires for optical applications, it is essential to synthesize nanowires that can emit predominantly via band to band or band edge (BE) transitions. However, many compound semiconductor nanowires, irrespective of the method of their growth, contain a high density of native defects; these result in competing deep defect (DD) related emission, limiting their utility for optoelectronic device applications. The concentration of these native defect states depends on the gas phase stoichiometry. In this work, we report on the influence of gas phase stoichiometry on the structural and optical properties of single crystal zinc selenide (ZnSe) nanowires. We find that nanowires grown under stoichiometric conditions contain such defect states with associated weak BE emission and strong DD emission. However, nanowires grown under Zn-rich conditions were characterized by photoluminescence spectra dominated by strong BE emission while those grown under Se-rich conditions showed strong DD related emission. Hence, it is necessary to develop a strategy for enhancing the BE emission while simultaneously quenching the DD emission. We demonstrate a technique of postgrowth treatment that can effectively perform this function, and using this strategy the ratio of the BE/DD emission can be increased by a factor of several thousands, at least an order of magnitude higher than previously reported values. This reveals BE dominated photoluminescence in these nanowires and makes these nanowires suitable for developing future optoelectronic devices.