gebhart/patrepeval huggingface dataset:
Gamma‐Vinyl GABA Decreases Voluntary Alcohol Consumption in Alcohol‐Preferring AA Rats (1993)

The effect of a GABA transaminase inhibitor, gamma-vinyl GABA, on the voluntary alcohol consumption of alcohol-preferring AA rats produced by selective breeding for high alcohol preference, was studied. The rats were first trained to voluntarily drink 10% (v/v) ethanol solution until their ethanol consumption stabilized. Gamma-vinyl GABA (100, 200 or 500 mg/kg) was then injected intraperitoneally in three groups of rats, with saline-injected animals serving as a control group. The rats continued to have a free choice between 10% ethanol and plain tap water for five days after the injection, and their ethanol, water and food consumptions were measured daily. Gamma-vinyl GABA decreased ethanol consumption by the rats in a dose-dependent way. The consumption remained significantly decreased for three days in the two groups receiving the highest doses, with only a small concomitant tendency to decreased food intake. The results suggest that an increase in brain GABA concentration decreases alcohol drinking, possibly through potentiation of the pharmacological action of ethanol.
Purification and characterization of thein vitroactivity of I-SceI, a novel and highly specific endonuclease encoded by a group I intron (1990)

Group I intron encoded proteins represent a novel class of site specific double strand endonucleases. The endonuclease activity of this class of proteins has been first demonstrated in vivo for I-Sce I which is encoded by a mitochondrial intron of Saccharomyces cerevisiae. Assays using crude cell extracts have shown that I-Sce I can be used in vitro as a restriction endonuclease potentially useful for recombinant DNA technology owing to its large recognition sequence (18 nucleotides). We report here the purification and the first detailed analysis of the in vitro activity and properties of I-Sce I.
Complexity and expression patterns of the desmosomal cadherins. (1992)

Desmosomes are intercellular junctions that contain two major kinds of transmembrane glycoproteins, desmoglein and desmocollins I and II, involved in cell-cell adhesion. Recent sequence analyses have shown that both desmosomal glycoproteins belong to the larger cadherin family of cell adhesion molecules, in which they represent two different subgroups characterized by their specific sequence and topogenesis. In analyses of cDNA sequences and Northern blot experiments we have now found that both desmoglein and desmocollins are not unique gene products but occur in different subtypes produced from different genes. Comparison of the complete amino acid sequences of type 1 and type 2 desmocollins and of two desmoglein subtypes shows considerable divergence. While the desmoglein genes can be differentially expressed in different cell types, both type 1 and type 2 desmocollins can coexist in the same cells of certain stratified epithelia as shown by in situ hybridization. We conclude that the cadherin composition of desmosomes is much more complex than assumed and can differ in the various epithelia.
Crystal Structure of GDP-Mannose Dehydrogenase:  A Key Enzyme of Alginate Biosynthesis in P. aeruginosa, (2003)

The enzyme GMD from Pseudomonas aeruginosa catalyzes the committed step in the synthesis of the exopolysaccharide alginate. Alginate is a major component of P. aeruginosa biofilms that protect the bacteria from the host immune response and antibiotic therapy. The 1.55 A crystal structure of GMD in ternary complex with its cofactor NAD(H) and product GDP-mannuronic acid reveals that the enzyme forms a domain-swapped dimer with two polypeptide chains contributing to each active site. The extensive dimer interface provides multiple opportunities for intersubunit communication. Comparison of the GMD structure with that of UDP-glucose dehydrogenase reveals the structural basis of sugar binding specificity that distinguishes these two related enzyme families. The high-resolution structure of GMD provides detailed information on the active site of the enzyme and a template for structure-based inhibitor design.
Grand challenges in stem cell treatments (2013)

SPECIALTY GRAND CHALLENGE article Front. Cell Dev. Biol., 10 October 2013Sec. Stem Cell Research https://doi.org/10.3389/fcell.2013.00002
Television Line 21 Encoded Information and its Impact on Receiver Design (1972)

A system has been proposed for the transmission of accurate time information during Line 21 of the vertical interval of standard television broadcasts. This paper will desctibe the basic system and its implementation in an approach to digital tuning. Many uses have been suggested for the vertical interval , including test signals, facsimile, etc. The National Bureau of Standards proposes using one of the lines for transmission of digitally encoded time information. The line chosen was originally line 1 but this was subsequently changed to line 21 due to the line 1 bypass used in broadcast processors. The information contained in the code would include -NBS time, local time, channel identification, 64 characters of text and a precise 1 MHz reference signal. The text is intended for deaf viewers, emergency codes, subtitles, network communications, etc.
Robust dialog state tracking using delexicalised recurrent neural networks and unsupervised adaptation (2014)

Tracking the user's intention throughout the course of a dialog, called dialog state tracking, is an important component of any dialog system. Most existing spoken dialog systems are designed to work in a static, well-defined domain, and are not well suited to tasks in which the domain may change or be extended over time. This paper shows how recurrent neural networks can be effectively applied to tracking in an extended domain with new slots and values not present in training data. The method is evaluated in the third Dialog State Tracking Challenge, where it significantly outperforms other approaches in the task of tracking the user's goal. A method for online unsupervised adaptation to new domains is also presented. Unsupervised adaptation is shown to be helpful in improving word-based recurrent neural networks, which work directly from the speech recognition results. Word-based dialog state tracking is attractive as it does not require engineering a spoken language understanding system for use in the new domain and it avoids the need for a general purpose intermediate semantic representation.
<title>Development of the halftone phase-shift mask for DUV exposure</title> (1996)

For bringing the halftone phase shift mask (HPSM) for 248 nm exposure into production, it is necessary to establish the mask-inspection method and mask-repair method. In this report, we will discuss the evaluation results of mask- inspection sensitivity test, comparison of mask repair methods and repaired-hole printability test. We fabricated a test mask that involves four kinds of programmed defect (clear defect; pinhole and protrusion, opaque defect; dot and intrusion), placed in 1.37 um hole array. Evaluation of defect sensitivity of KLA301 inspection system and defect printability using aerial image measurement system (AIMS) were done. Based on these results, we conclude that defect sensitivity for dot and edge defect (protrusion or intrusion) was slightly poor. We couldn't evaluate the sensitivity of pinhole. Because we could not fabricate pinholes smaller than 0.48 um and all programmed pinholes were detected. In respect of repair of clear defect, we could obtain a printability equal to holes without defects by deposition of carbon patch on the clear defect area, using a focused-ion beam (FIB). Three repairing methods of opaque defect (Laser-zapper, FIB, gas-assisted-FIB (GA-FIB)) were evaluated. Based on results of printability test using AIMS, we confirmed that GA-FIB is the best method for repairing of opaque defect. And we also report the ability of our HPSM using a chromium fluoride for ArF (193 nm) exposure.
Effects of IL-12 and IL-23 on Antigen-Presenting Cells at the Interface Between Innate and Adaptive Immunity (2002)

Recent evidence from different experimental systems has demonstrated that autocrine activation of antigen-presenting cells (APCs) may be important at the initiation of an immune response, and that a specific set of cytokines may meet the dual needs of activating APCs and priming and/or maintaining the antigen-specific T-cell response. Composite factors with p40, including IL-12 (p40/p35) and IL-23 (p40/p19), may be two such immunoregulatory cytokines, their effects encompassing actions on both myeloid APCs and T cells. However, although both cytokines enhance the Th1 costimulatory functions of APCs, and IL-23 does induce IL-12 from APCs, their effects, which in part overlap, can be differentiated from one another. This review summarizes recent data on the actions of IL-12 and IL-23 on dendritic cells and macrophages at the interface between innate and adaptive immunity.
The Quantum Mechanics Of Larger Semiconductor Clusters ( (1990)

Machine learning (ML) is transforming all areas of science. The complex and time-consuming calculations in molecular simulations are particularly suitable for an ML revolution and have already been profoundly affected by the application of existing ML ...Read More
Alkylation of Benzene with Dodecene-1 Catalyzed by Supported Silicotungstic Acid (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAlkylation of Benzene with Dodecene-1 Catalyzed by Supported Silicotungstic AcidRaynor T. Sebulsky and Alfred M. HenkeCite this: Ind. Eng. Chem. Process Des. Dev. 1971, 10, 2, 272–279Publication Date (Print):April 1, 1971Publication History Published online1 May 2002Published inissue 1 April 1971https://pubs.acs.org/doi/10.1021/i260038a022https://doi.org/10.1021/i260038a022research-articleACS PublicationsRequest reuse permissionsArticle Views289Altmetric-Citations44LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Touchdown Enzyme Time Release-PCR for Detection and Identification of Chlamydia trachomatis , C. pneumoniae , and C. psittaci Using the 16S and 16S-23S Spacer rRNA Genes (2000)

Three touchdown enzyme time release (TETR)-PCR assays were used to amplify different DNA sequences in the variable regions of the 16S and 16S-23S spacer rRNA genes specific for Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci as improved tests for sensitive diagnosis and rapid species differentiation. The TETR-PCR protocol used 60 cycles of amplification, which provided improved analytical sensitivity (0.004 to 0.063 inclusion-forming unit of Chlamydia species per PCR). The sensitivity of TETR-PCR with primer set CTR 70-CTR 71 was 96.7%, and the specificity was 99.6%, compared to those of the AMPLICOR PCR for the detection of C. trachomatis in vaginal swab samples. TETR-PCR for C. pneumoniae with primer set CPN 90-CPN 91 was 90% sensitive and 93.3% specific compared with a nested PCR with primer set CP1/2-CPC/D for clinical respiratory samples. TETR-PCR for C. psittaci with primer set CPS 100-CPS 101 showed substantial agreement with cell culturing (kappa, 0.78) for animal tissue samples. Primer sets were then combined into a single multiplex TETR-PCR test. The respective 315-, 195-, and 111-bp DNA target products were precisely amplified when DNA from each of the respective Chlamydia species or combinations of them was used. Multiplex chlamydia TETR-PCR correctly identified one strain of each of the 15 serovars of C. trachomatis, 22 isolates of C. pneumoniae, and 20 isolates of C. psittaci. The primer sets were specific for each species. No target products were amplified when DNA from C. pecorum or a variety of other microorganisms was tested for specificity. TETR-PCR with primers selected for specific sequences in the 16S and 16S-23S spacer rRNA genes is a valuable test that could be used either with individual primers or in a multiplex assay for the identification and differentiation of Chlamydia species from culture isolates or for the detection of chlamydiae in clinical samples.
Arterial reconstruction with vascular clips is safe and quicker than sutured repair (1998)

Arterial anastomoses performed with vascular-closure staple clips are faster than sutured anastomoses, and result in comparable wound healing when assessed for patency, tensile strength, degree of narrowing and intimal reaction.
Structural Principles of Tau and the Paired Helical Filaments of Alzheimer’s Disease (2007)

Tau, a major microtubule-associated protein in brain, forms abnormal fibers in Alzheimer's disease and several other neurodegenerative diseases. Tau is highly soluble and adopts a natively unfolded structure in solution. In the paired helical filaments of Alzheimer's disease, small segments of tau adopt a beta-conformation and interact with other tau molecules. In the filament core, the microtubule-binding repeat region of tau has a cross-beta structure, while the rest of the protein retains its largely unfolded structure and gives rise to the fuzzy coat of the filaments.
IL-1-induced Post-transcriptional Mechanisms Target Overlapping Translational Silencing and Destabilizing Elements in IκBζ mRNA* (2010)

The inflammatory cytokine IL-1 induces profound changes in gene expression. This is contributed in part by activating translation of a distinct set of mRNAs, including IκBζ, as indicated by genome-wide analysis of changes in ribosomal occupancy in IL-1α-treated HeLa cells. Polysome profiling of IκBζ mRNA and reporter mRNAs carrying its 3' UTR indicated poor translation in unstimulated cells. 3' UTR-mediated translational silencing was confirmed by suppression of luciferase activity. Translational silencing was unaffected by replacing the poly(A) tail with a histone stem-loop, but lost under conditions of cap-independent internal initiation. IL-1 treatment of the cells caused profound shifts of endogenous and reporter mRNAs to polysome fractions and relieved suppression of luciferase activity. IL-1 also inhibited rapid mRNA degradation. Both translational activation and mRNA stabilization involved IRAK1 and -2 but occurred independently of the p38 MAPK pathway, which is known to target certain other post-transcriptional mechanisms. The translational silencing RNA element contains the destabilizing element but requires additional 5' sequences and is impaired by mutations that leave destabilization unaffected. These differences in function are associated with differential changes in protein binding in vitro. Thus, rapid degradation occurs independently of the translational silencing effect. The results provide evidence for a novel mode of post-transcriptional control by IL-1, which impinges on the time course and pattern of IL-1-induced gene expression.
Fibromyalgia Syndrome and Disease Activity in Systemic Lupus Erythematosus (1994)

To study the effect of fibromyalgia syndrome (FS) on the expression of systemic lupus erythematosus (SLE) and on the measurement of disease activity, we performed a cross-sectional study of subjects with SLE. Eighty-seven subjects were studied and 22 (25.3%), all female, had FS (Yunus' criteria). Disease activity and organ system involvement were assessed using the systemic lupus activity measure (SLAM) and using 10 cm visual analogue scales (VAS) completed by both physician and patient. No significant difference between FS and non-FS groups in the objective measurement of disease activity was present, median (range) SLAM scores being 5 (0-18) and 6 (0-24), respectively (NS). Similarly, expression of SLE, as measured by the prevalence of specific organ system involvement, was similar in the two groups. In contrast the prevalence of glucocorticoid use, antibodies to DSDNA and fulfilling four ACR criteria was higher in the non-FS group. The rating of SLE disease activity was affected by concomitant FS. Physician and patient disease activity VAS correlated significantly with SLAM scores in non-FS subjects (P < 0.001, Spearman analysis), whereas in FS subjects, neither physician nor patient VAS correlated with SLAM scores. We conclude that FS is prevalent in individuals with SLE and does not affect disease expression but may interfere with the rating of disease activity.
Increased Sensitivity to Etomidate in the Elderly (1986)

To determine the effect of aging on the pharmacokinetics and pharmacodynamics of etomidate, we administered etomidate (5 to 10 mg/min) by intravenous infusion to 21 healthy surgical patients, age 22 to 82 yr. Etomidate produced progressive slowing of the EEG to an easily recognized pattern (stage 3) that determined the dosage endpoint. Subsequent power-spectrum analysis of the EEG gave the median frequency. Median frequency values and simultaneous measurements of blood etomidate concentration were incorporated into a sigmoid Emax pharmacodynamic model that permitted an estimate of IC50, the blood etomidate concentration which produced a 50% reduction in the median frequency. The dose of etomidate required to reach the uniform EEG endpoint decreased significantly with increasing age (r2 = .68) as did the dose needed to produce maximal median frequency depression (r2 = .69). None of the parameters of the pharmacodynamic effect model, including IC50, correlated with age, suggesting that increased brain sensitivity in the elderly does not cause the age-related change in dose requirement. The initial distribution volume for etomidate decreased significantly with increasing age (r = .56), implying that a higher initial blood concentration in the elderly following any given dose of etomidate is part of the cause of the lower dose requirement in the elderly patient. A contracted initial distribution volume in the elderly may result from well described physiologic changes of age. Etomidate clearance also decreased with age. Age-dependent changes in etomidate pharmacokinetics rather than altered brain responsiveness may be the basis for the decreased etomidate dose requirement in the elderly.
Circulating and Tissue Angiotensin Systems (1987)

No AccessJournal of Urology1 Jul 1987Circulating and Tissue Angiotensin Systems D.J. Campbell D.J. CampbellD.J. Campbell More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)43075-8AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Circulating and Tissue Angiotensin Systems." The Journal of Urology, 138(1), p. 227 © 1987 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 138Issue 1July 1987Page: 227 Advertisement Copyright & Permissions© 1987 by The American Urological Association Education and Research, Inc.MetricsAuthor Information D.J. Campbell More articles by this author Expand All Advertisement PDF downloadLoading ...
DryadLINQ: a system for general-purpose distributed data-parallel computing using a high-level language (2008)

DryadLINQ is a system and a set of language extensions that enable a new programming model for large scale distributed computing. It generalizes previous execution environments such as SQL, MapReduce, and Dryad in two ways: by adopting an expressive data model of strongly typed .NET objects; and by supporting general-purpose imperative and declarative operations on datasets within a traditional high-level programming language.A DryadLINQ program is a sequential program composed of LINQ expressions performing arbitrary side-effect-free transformations on datasets, and can be written and debugged using standard .NET development tools. The DryadLINQ system automatically and transparently translates the data-parallel portions of the program into a distributed execution plan which is passed to the Dryad execution platform. Dryad, which has been in continuous operation for several years on production clusters made up of thousands of computers, ensures efficient, reliable execution of this plan.We describe the implementation of the DryadLINQ compiler and runtime. We evaluate DryadLINQ on a varied set of programs drawn from domains such as web-graph analysis, large-scale log mining, and machine learning. We show that excellent absolute performance can be attained--a general-purpose sort of 1012 Bytes of data executes in 319 seconds on a 240-computer, 960- disk cluster--as well as demonstrating near-linear scaling of execution time on representative applications as we vary the number of computers used for a job.
Apis mellifera has two isoforms of cytoplasmic HSP90 (2010)

Unlike most other insects, annotated genomic data suggest that Apis mellifera has two homologous copies of the cytoplasmic gene HSP90. In this study, we did a phylogenetic analysis on these two copies with some other insects HSP90 genes, and we also manipulated a reverse transcript (RT)-PCR to find all the putative transcripts for both copies. Phylogenetic analysis indicates that A. mellifera possesses two isoforms of cytoplasmic HSP90: The 'traditional' isoform clusters with cytoplasmic HSP90 of other insects. The other isoform, which occurs phylogenetically as the sister group of all insects, may be a new gene and specific to A. mellifera. The results of RT-PCR indicate that this new isoform contains at least eight transcripts derived from the same genomic locus by complicated alternative splicing (GenBank accession numbers: FJ713701, FJ713702, FJ713703, FJ713704, FJ713705, FJ713706, FJ713707 and FJ713708, respectively). The existence of this specific HSP90 might be related to the caste differentiation of bees.