gebhart/patrepeval huggingface dataset:
Novel anti-CD3 chimeric antigen receptor targeting of aggressive T cell malignancies (2016)

Peripheral T-cell lymphomas (PTCLS) comprise a diverse group of difficult to treat, very aggressive non-Hodgkin's lymphomas (NHLS) with poor prognoses and dismal patient outlook. Despite the fact that PTCLs comprise the majority of T-cell malignancies, the standard of care is poorly established. Chimeric antigen receptor (CAR) immunotherapy has shown in B-cell malignancies to be an effective curative option and this extends promise into treating T-cell malignancies. Because PTCLS frequently develop from mature T-cells, CD3 is similarly strongly and uniformly expressed in many PTCL malignancies, with expression specific to the hematological compartment thus making it an attractive target for CAR design. We engineered a robust 3rd generation anti-CD3 CAR construct (CD3CAR) into an NK cell line (NK-92). We found that CD3CAR NK-92 cells specifically and potently lysed diverse CD3+ human PTCL primary samples as well as T-cell leukemia cells lines ex vivo. Furthermore, CD3CAR NK-92 cells effectively controlled and suppressed Jurkat tumor cell growth in vivo and significantly prolonged survival. In this study, we present the CAR directed targeting of a novel target - CD3 using CAR modified NK-92 cells with an emphasis on efficacy, specificity, and potential for new therapeutic approaches that could improve the current standard of care for PTCLs.
Adenosine receptors in the nervous system (1989)

Purine receptors, G.Burnstock purification and reconstitution of the A1 adenosine receptor, J.Linden and R.Munshi monoclonal auto-anti-idiotypic antibodies to andenosine receptors, H.H.Ku et al modualtion of 3HDPCPX binding to membrane bound and solubilized A1 andenosine receptors by guanine nucleotides, K.N.Klotz et al molecular probes for andenosine receptors, J.W.Daly and K.A.Jacobson allosteric enhancers of andenosine A1 receptor binding and function, R.F.Burns and J.H.Fergus andenosine receptor ligands as therapeutic entities - molecular specificity in relation to functional and therapeutic activity, M.Williams et al characterization of the adenosine receptor that mediates inhibition of transmitter release at the frog neuromuscular junction, J.A.Ribeiro and A.M.Sebastiao function and structure of presynaptic andenosine receptors in peripheral adrenergic and cholinergic neurons, D.M.Paton magnesium-dependency of presynaptic inhibition by andenosine, T.W.Stone and J.T.Bartrup sensitivity to Mg++ of ligand binding to A1 adenosine receptors in ray brain and adipocytes, M.Reddington et al regulatory properties of andenosine A1 receptors in the CNS and peripheral tissues, D.M.F.Cooper and C.L.Boyajian pertussis toxin-sensitive G proteins and adenosine receptors in the central nervous system, J.W.Phillis mechanisms of inhibition of transmitter release by adenosine receptor activation, B.B.Fredholm et al on the transducing mechanism operated by the adenosine receptor mediating inhibition of transmitter release at the neuromuscular junction, A.M.Sebastiao calcium-dependent acetylcholine secretion - influence of adenosine, E.M.Silinsky et al inhibition of neuronal calcium currents by adenosine - role of G-proteins, R.H.Scott and A.C.Dolphin functional correlates of synaptic and extrasynaptic adenosine receptors in the hippocampus, P.Schubert and R.Mager exogenous and endogenous adenosine actions in the hippocampus, H.L.Haas and R.W.Greene potassium currents coupled to an A1 receptor by a G-protein in cultured central neurons, L.O.Trussell and M.B.Jackson.
Mycelium Biking : Eco-design at its best (2016)

Mycelium Biking, a master thesis project about using mycology in eco-design made by design engineering student Alexander Wagner. The present global ecology drives researchers to find environmentally friendly alternatives to commonly used materials such expanded plastics and concrete. The project examined the possibilities of implementing mycelium materials in systematic product development processes. The eligibility of the material was tested by developing products for electric cargo bikes and, by doing so, reduce their ecological footprint. Mycelium materials relevant to Industrial Design Engineering mainly because of their ecological qualities but also because of their performance and the wide range of applicable fields. This study presents six different products developed for the cargo bike in which mycelium materials was considered eligible. The study also presents the material in general showcasing the applicability for other types of products. It was found most applicable for lightweight products used in friendly environments and with low demands on tolerances. These types of product don’t need additional coating, are self-compostable, are possible to create with no manufacturing costs and its production close-to carbon neutral. Conclusively, any product developer in need of a material with an elastic modulus of 15MPa for densities 100kg/m3 (These are the properties of untreated mycelium materials grown without pressure) should consider using mycelium materials. Designers have the option to design a product with a manufacturing process that need no supplementary energy and without any ecological footprint (should you assume availability of local feedstock). The ecological and economic benefits of such material are obvious and it is up to the designer to weigh them against the issues related to using the material (shrinkage, coating & quality).
Holographic methods in X-ray crystallography. II. Detailed theory and connection to other methods of crystallography (1993)

Equations are derived for finding an unknown part of the electron density in the unit cell of a crystal when part of the structure of its contents is already known. These equations are based on the similarity of the X-ray diffraction pattern to a hologram. The X-ray field scattered by the known part of the structure is identified as the holographic reference beam. It interferes with the waves scattered from the unknown part of the structure. The interference pattern contains phase information that can be exploited to recover fully the unknown part of the structure. This paper discusses mathematical properties of the resulting equations and some methods for their solution. A strong similarity to inverse problems of image processing is pointed out and connections to other known methods of X-ray crystallography are established. In paper III [Maalouf, Hoch, Stem, Szöke &amp; Szöke (1993). Acta Cryst. A 49 , 866–871], some modest numerical simulations are presented.
Polyphase quadrature filters--A new subband coding technique (2005)

A new method of implementing filter banks for subband coding of speech is presented. First, Esteban's Quadrature Mirror Filter principle is extended to allow the direct synthesis of filter banks with any number of equal size filter bands. Then, by combining the quadrature filter characteristic with the polyphase network implementation of filter banks, a new filter bank structure is obtained which requires 35% fewer computations than existing designs. In this paper the theory of operation of the Polyphase Quadrature Filter is presented and techniques for its efficient implementation are described. Then, examples of filter banks using this approach are shown and compared with other designs, and a simulation of a 16 Kbits/s coder using these filters is presented.
Activation signals are delivered through two distinct epitopes of CD100, a unique 150 kDa human lymphocyte surface structure previously defined by BB18 mAb (1995)

We have previously reported the identification of a glycoprotein dimer, now termed CD100, at the lymphocyte cell surface using a mAb termed BB18. In the present study we isolated a second mAb, termed BD16, recognizing another epitope of this 150 kDa structure. We show that interaction with individual mAb triggers differential functional effects on peripheral blood lymphocytes (PBL) and purified T cells. In particular and of the utmost interest, BD16 mAb strongly inhibits CD3 induced PBL proliferation while it dramatically increases the CD2 induced proliferation. In contrast, after T cell purification, BD16 mAb increases both CD2 and CD3 induced proliferation. It has to be noted that perturbation of the same 150 kDa surface molecule with BB18 mAb exerts no effect on CD2 and CD3 induced proliferations, while inducing a strong lymphocyte proliferation in the presence of submitogenic concentrations of phorbol myristate acetate.
PSYCHOLOGY IN THE POST HUMAN GENOME ERA (2002)

What is the role of behavioral genetics and psychology in general in the post human genome era? What do we really know? First oﬀ, HUGO has given us a map with addresses, and perhaps who lives at those addresses, but even less idea of the occupations of the occupants. In other words, even though we have a genetic sequence, we have very little knowledge concerning the functions of the many potential variants at each genetic “address” or locus. Nonetheless, psychology has already played an important role, and should play an even greater role in the future of understanding how genes function.
Comparative thermodynamic analyses of the Fv, Fab* and Fab and Fab fragments of anti‐dansyl mouse monoclonal antibody (1995)

In order to investigate the role of the constant domains on the antigen-binding property of the variable domains, we have carried out a comparative thermodynamic study of the anti-dansyl Fv, Fab* and Fab fragments that possess the identical amino acid sequence of the variable domains. The thermodynamic analyses have shown that binding constants, enthalpy changes and entropy changes are similar for the three antigen-binding fragments, whereas the thermal stability of Fab is much higher than that of Fv and Fab*. We have concluded that (i) the variable domains of the three antigen-binding fragments possess identical intrinsic capability for antigen binding and (ii) the two constant domains serve to improve the stability of the variable domains.
Modern Ceramic Engineering (2005)

Ceramic materials have proven increasingly important in industry and in the fields of electronics, communications, optics, transportation, medicine, energy conversion and pollution control, aerospace, construction, and recreation. Professionals in these fields often require an improved understanding of the specific ceramics materials they are using
Prediction of surface and interior regions in proteins--Part I: Linear tripeptide sequences identify structural boundaries in proteins. (1992)

Previously, using an IBM or compatible program called SurfacePlot, it was shown that amino acid parameters of hydrophilicity, accessibility and flexibility could successfully predict linear accessible or surface regions observed in the x-ray structure of proteins. In addition, these surface regions defined the boundaries of secondary structure regions in proteins. In this paper we show that tripeptides containing the polar residues G K S D E N P T A R and Q also predict surface regions of proteins. Although tripeptides containing these residues predicted 91% of the observed accessible regions, the combination of polar tripeptide predictions with SurfacePlot predictions significantly reduced overpredictions (36% to 17%) and improved accuracy from 71% to 83% while maintaining an 86% prediction rate of observed x-ray-defined linear accessible regions. This new program also predicted 86% of beta-turn regions. The data indicated that not all beta-turns are highly exposed to solvent and that beta-turns should not be used as the sole criteria for defining surface regions.
Non‐invasive Vagus Nerve Stimulation Acutely Improves Blood Pressure Control in a Placebo Controlled Study (2017)

Background Despite positive outcomes across a variety of disease states, invasive vagus nerve stimulation (VNS) has been associated with adverse side‐effects. Given this, non‐invasive VNS (tVNS) via the auricular branch of the vagus nerve (ABVN) has been proposed as an alternative approach. However, the mechanisms underlying these positive outcomes remain unclear. Therefore, we controlled the sensory effects (placebo) to investigate if tVNS acutely improves blood pressure (BP) control. Methods Thirteen healthy men (23±1yrs) were randomized across three experimental visits in a random order. In active tVNS, electrodes were placed on the tragus of the ear and electrical current was applied by using a Transcutaneous Electrical Nerve Stimulation device. A time‐ control visit was performed with the electrodes placed on tragus, but no current was applied (sham‐T). Additionally, to avoid a confounding sensory effect, another sham protocol was performed with same electrical current of the active visit, but the electrodes were placed on the ear lobe (an area without cutaneous nerve endings from the vagus – sham‐L). Before interventions were applied, we inform to subjects that both active tVNS and sham‐L would not cause any harm. Beat‐to‐beat heart rate (HR) and BP were monitored at rest, during tVNS (active, sham‐T and sham‐L) and recovery. Spontaneous cardiac baroreflex sensitivity (cBRS) was measured via sequence technique. Both HR (LF/HF) and BP variability (BPV) were also measured. Results Arterial BP and BPV were not affected by any active or sham protocols (P&gt;0.05). HR and LF/HF ratio decreased (Δ–3.4±1% and Δ–17±10%, P&lt;0.05, respectively) and cBRS increased (Δ20±7%, P&lt;0.05) only during active tVNS, but were unchanged during both sham protocols (P&gt;0.05). Conclusion The results of the present study provide evidence that tVNS acutely improves neural control of BP in healthy young men. These findings allow us to suggest that spontaneous cBRS and cardiac sympathovagal balance could play a role in the mechanisms involved in previously reported beneficial outcomes caused by tVNS. Future studies are needed to confirm these findings in older and diseased populations. Support or Funding Information J.L.S., A.L.T., P.M.M. have been supported by scholarships from Coordination for the Improvement of Higher Education Personnel (CAPES).
Revealing the Atomic Restructuring of Pt–Co Nanoparticles (2014)

We studied Pt-Co bimetallic nanoparticles during oxidation in O2 and reduction in H2 atmospheres using an aberration corrected environmental transmission electron microscope. During oxidation Co migrates to the nanoparticle surface forming a strained epitaxial CoO film. It subsequently forms islands via strain relaxation. The atomic restructuring is captured as a function of time. During reduction cobalt migrates back to the bulk, leaving a monolayer of platinum on the surface.
Differential scanning calorimetry studies on poly(ethylene glycol) with different molecular weights for thermal energy storage materials (2002)

Abstract Melting and crystallization behaviour of poly(ethylene glycol) (PEG) with different molecular weights (from 1000 to 35 000) and chosen blends of PEG is investigated by means of differential scanning calorimetry (DSC) operating in dynamic mode at different heating rates. The influence of the molecular weight of PEG on its melting point and enthalpy of fusion is evaluated; from the DSC data the degree of crystallinity is calculated–it is found that there is an increased tendency of higher‐molecular‐weight PEGs towards the formation of crystalline phase owing to their lower segmental mobility and more convenient geometrical alignment. During the freezing cycle, an increase in the molecular weight of PEG causes an increase in the solidification temperature and heat of crystallization. Thermal transition data are supplemented by optical microscopy–numerous small sphere‐shaped crystalline structures are observed to join together and impinge on their neighbours, forming eventually a multilayered lamellar texture. By implementing second polymeric component the polydispersity of the system increases, thus lowering the crystallization degree during preparation phase. It influences also the course of solidification by lowering the crystallization temperature, T c . An additional effect observed in the case of the blend's freezing is associated with larger supercooling, probably due to morphological constraints and entanglements in interlamellar regions. The possible advantage of using PEG blends to replace pure components is connected with the possibility of changing the temperature range and heat associated with melting/freezing. Copyright © 2003 John Wiley &amp; Sons, Ltd.
A neural network expert system for diagnosing and treating hypertension (1991)

Hypernet (Hypertension Neural Expert Therapist), a neural network expert system for diagnosing and treating hypertension, is described. After a brief look at artificial neural networks, the authors describe the structure of the three modules that make up Hypernet, starting with the specific problem each network is intended to solve and explaining how the network is expected to operate. The tools developed for implementing the system are a compiler for a simple descriptive language that enables the authors to define, train, and test networks; a graphic editor that translates the network drawn by the user into the proper statements; and a set of programs for interactively generating the examples. The data used as examples, the learning phase, and the results of tests for evaluating the performance of each network are described. and conclusions about overall system results are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Alveolar type II-like cells release G-CSF as neutrophil chemotactic activity (1998)

We evaluated the potential of A549 cells, an alveolar type II epithelial cell line, to release granulocyte colony-stimulating factor (G-CSF), in addition to interleukin (IL)-8 and leukotriene B4, as neutrophil chemotactic activity (NCA). Human recombinant IL-1beta stimulated A549 cells to release NCA in a time- and dose-dependent fashion. The released NCA was blocked by mouse anti-human G-CSF polyclonal antibody. Molecular-sieve column chromatography revealed that IL-1beta induced the release of a 19- to 20-kDa chemotactic mass that was inhibited by anti-human G-CSF antibody. IL-1beta stimulated the release of G-CSF in a dose-dependent fashion, but the time-dependent profile of G-CSF showed that the concentration of G-CSF declined after 48 h. Tumor necrosis factor (TNF)-alpha, Escherichia coli lipopolysaccharide (LPS), and bradykinin (BK) stimulated A549 cells to release NCA that was inhibited by anti-G-CSF antibody. The release of G-CSF in response to TNF-alpha, LPS, and BK was significantly increased. The similar concentrations of human recombinant G-CSF (10-1,000 pg/ml) as in the supernatant fluid induced neutrophil chemotaxis. G-CSF mRNA was expressed time and dose dependently at 4 h and declined after 4 h in response to IL-1beta as evaluated by RT-PCR. The expression of G-CSF mRNA was also observed by TNF-alpha, LPS, and BK stimulation. These data suggest that type II alveolar epithelial cells may produce G-CSF as NCA and may participate in the regulation of leukocyte extravasation.
The Predicted Structure of Immunoglobulin D1.3 and Its Comparison with the Crystal Structure (1986)

Predictions of the structures of the antigen-binding domains of an antibody, recorded before its experimental structure determination and tested subsequently, were based on comparative analysis of known antibody structures or on conformational energy calculations. The framework, the relative positions of the hypervariable regions, and the folds of four of the hypervariable loops were predicted correctly. This portion includes all residues in contact with the antigen, in this case hen egg white lysozyme, implying that the main chain conformation of the antibody combining site does not change upon ligation. The conformations of three residues in each of the other two hypervariable loops are different in the predicted models and the experimental structure.
Pastoral care for children conceived through IVF (2012)

Passing on Catholic teaching effectively means exercising both the doctrinal and pastoral ministries of a Church which professes to be both Mother and Teacher.
Decreased brown fat markedly enhances susceptibility to diet-induced obesity, diabetes, and hyperlipidemia. (1996)

Previous studies have indicated that rodents are relatively resistant to diet-induced obesity and that this resistance may be mediated in part by the capacity for diet-induced thermogenesis in brown adipose tissue (BAT). To test this hypothesis, we fed UCP-DTA transgenic with toxigene-mediated ablation of BAT and their control littermates a "Western diet" [21% (wt/wt) fat] or normal mouse chow [6.5% (wt/wt) fat]. The diets were begun at weaning (19 days old). At the age of 12 weeks, transgenic mice receiving the Western diet were markedly obese. The increased body weight and total body lipid content were significantly greater in transgenic mice receiving the Western diet than were the additive individual effects of Western diet (in control mice) and decreased BAT (in chow-fed mice), suggesting a synergistic interaction between diminished BAT and diet. A synergistic effect of Western diet and BAT ablation was also observed for morbid metabolic complications, such as insulin resistance, hyperglycemia, and hyperlipidemia. These metabolic changes were accompanied by increased expression of tumor necrosis factor-alpha and decreased expression of GLUT4 and beta 3-adrenergic receptor messenger RNA levels in white adipose tissue of UCP-DTA transgenic mice receiving the Western diet compared to those in the other experimental groups. As previously described, transgenic mice with diminished brown fat are hyperphagic. Of note, the degree of hyperphagia in transgenics compared to controls was similar whether the animals were fed chow or a Western diet. Thus, the synergistic effect of Western diet on obesity in transgenic mice was not mediated by a further stimulation of food intake. Overall, this study demonstrates the existence of a synergistic interaction between decreased BAT and Western diet to cause marked obesity and its accompanying disorders, such as insulin resistance and hyperlipidemia, and gives further support for the view that an important function of BAT is protection from diet-induced obesity, diabetes, and insulin resistance.
Type 2 diabetes mellitus in childhood: obesity and insulin resistance. (2008)

As rates of childhood obesity climb, type 2 diabetes mellitus has increasingly been diagnosed in children and adolescents, with the highest incidence occurring among youth from racial and ethnic minority backgrounds. The serious complications associated with type 2 diabetes mellitus make it essential for physicians to be aware of risk factors and screening guidelines, allowing for earlier patient diagnosis and treatment. It is also important for physicians to be aware of the treatment options available, including weight control through diet and exercise as well as common pharmacotherapeutic options.
Automated Multisensor Registration: Requirements And Techniques (2005)

A b s t r a c t A necessary first step in the fusion of data from a number of different remote sensors is the correction of the systematic geo-metric distortion characteristic of each sensor followed by a pre-cision registration to remove any residual random offsets. This paper describes our approach to automated multisensor registra-tion. The effects of spatially, temporally and spectrally varying factors which influence image dynamics are reviewed. A speci-fication of the requirements for an operational algorithm is for-mulated using these factors. Additionally, the structure of an efficient, automated system is defined. A number of candidate image processing techniques are evaluated within this structure using a multisensor test data set assembled from the Landsat TM, SEASAT and SPOT sensors. The results are presented and discussed.