gebhart/patrepeval huggingface dataset:
A Survey on Methods and Theories of Quantized Neural Networks (2018)

Deep neural networks are the state-of-the-art methods for many real-world tasks, such as computer vision, natural language processing and speech recognition. For all its popularity, deep neural networks are also criticized for consuming a lot of memory and draining battery life of devices during training and inference. This makes it hard to deploy these models on mobile or embedded devices which have tight resource constraints. Quantization is recognized as one of the most effective approaches to satisfy the extreme memory requirements that deep neural network models demand. Instead of adopting 32-bit floating point format to represent weights, quantized representations store weights using more compact formats such as integers or even binary numbers. Despite a possible degradation in predictive performance, quantization provides a potential solution to greatly reduce the model size and the energy consumption. In this survey, we give a thorough review of different aspects of quantized neural networks. Current challenges and trends of quantized neural networks are also discussed.
Intracellular Mechanisms Regulating Exocytotic Secretion in Mast Cells (1991)

The release of inflammatory mediators from mast cells occurs by a regulated exocytotic process. We have been able to study the intracellular events in this pathway by permeabilizing the plasma membrane of rat peritoneal mast cells and stimulating exocytosis by providing both Ca2+ and a guanine nucleotide. By this approach we have obtained evidence for the participation of at least two guanine nucleotide binding proteins in the control of exocytosis. We have also shown that ATP is unnecessary for the final events, but that it does have a number of modulatory functions, for instance in the control of the effective affinity of the proteins that bind Ca2+ and GTP. There is also evidence for a protein dephosphorylation in the later stages of the control pathway.
Universal Quantum Simulators (1996)

Feynman's 1982 conjecture, that quantum computers can be programmed to simulate any local quantum system, is shown to be correct.
Developing Civic Agency in an International Videoconference: Reflecting on Audience and Public Voice in Participatory Media (2013)

This article presents findings from a multisite case study that focused on three international videoconferences, which involved secondary students discussing controversial social issues. As a learning experience, the videoconferences helped students better understand their use of participatory media, authentic discussion, and public voice. Findings suggested that the students’ engagement in dialogue with a diverse audience of peers resulted in students reflecting on the quality of their classroom discussions and their regular interactions with participatory media. By engaging in authentic discussion with peers outside of their typical school and social circles, students’ better understood the purpose of their public voice. After reflectively comparing the videoconference experience to other participatory media and learning experiences, the students made decisions to use participatory media in the future to construct similar media-based authentic learning experiences. Thus, participation in the videoconference helped students consider and develop their civic agency. Implications for social studies educators are discussed.
Cutaneous rat wounds expressC49a, a novel gene with homology to the human melanoma differentiation associated gene,Mda-7 (1999)

We have used DD-PCR (differential display-polymerase chain reaction) to identify new genes that are over- or underexpressed during wound repair. DD-PCR performed on excisional wounds identified the expression of rat c49a. Cloning and sequence analysis of the rat c49a gene revealed high homology to a novel human melanoma differentiation associated gene, mda-7. The human mda-7gene isolated from melanoma cell lines, has been linked with human melanoma differentiation, and growth suppression. Moreover, transfection of human mda-7 constructs into human tumor cells suppresses the growth and colony formation of tumor cells from diverse origins. To confirm and relatively quantitate expression of rat c49a gene during repair, specific primer, reduced cycle RT-PCR (reverse transcription-PCR) was performed. RT-PCR showed an approximately 9 to 12-fold elevation of rat c49a mRNA at 12 h to 5 days above nonwounded controls that gradually decreased to approximately 1.5 to 3-fold by day 14. Cloning and sequence analysis of the entire 1200 base pair c49a gene product showed 78% nucleotide homology to human mda-7. Immunohistochemistry studies localized rat C49A expression primarily to fibroblast-like cells at the wound edge and base. The marked up-regulation of rat c49a transcripts during the inflammatory and early granulation tissue phases of wound repair where cellular processes such as re-epithelialization, angiogenesis, and fibroplasia predominate--suggest that c49a is associated with proliferation of fibroblasts in wound healing.
An internal model approach to (optimal) frequency regulation in power grids (2014)

This paper studies the problem of frequency regulation in power grids under unknown and possible time-varying load changes, while minimizing the generation costs in some particular cases. We formulate this problem as an optimal<br/>output agreement problem for distribution networks. This problem has been recently addressed by some of the authors using incremental passivity and dynamic internal-model-based controllers. We believe that this framework is general enough to allow for more complex control scenarios in future extensions.
Evaluation of the Antifungal Activity of Natural Xanthones from Garcinia mangostana and Their Synthetic Derivatives (1997)

The antifungal activity of several xanthones isolated from the fruit hulls of Garcinia mangostana and some derivatives of mangostin against three phytopathogenic fungi, Fusarium oxysporum vasinfectum, Alternaria tenuis, and Dreschlera oryzae, has been evaluated. The natural xanthones showed good inhibitory activity against the three fungi. Substitution in the A and C rings has been shown to modify the bioactivities of the compounds.
Evaluation of Three Cell Culture Systems as Substrates for Influenza Virus Assay (1970)

The propagation of various influenza virus strains in primary rhesus monkey kidney (RMK), primary hamster kidney (HK), and the MDCK-USD canine kidney (CK) cell cultures was compared. Virus-infected cultures were examined for cytopathic effect (CPE) and by performing the hemadsorption (HAd) technique. The highest HAd titers were found most often in HK, followed by RMK and CK. However, the CK cell line provided the best substrate for detecting CPE. Although influenza B strains tended to grow to higher titers, these strains produced less CPE than did the A strains.
Pathogenesis of experimentally induced feline immunodeficiency virus infection in cats (1988)

Feline immunodeficiency virus (FIV; formerly, feline T-lymphotropic lentivirus) is a typical lentivirus resembling human and simian immunodeficiency viruses in morphologic features, protein structure, and reverse transcriptase enzyme. It is antigenically dissimilar, however. The virus is tropic for primary and permanent feline T-lymphoblastoid cells and Crandell feline kidney cells. The virus did not grow in other permanent feline non-lymphoblastoid cells that were tested, or in lymphoid and non-lymphoid cells from man, dogs, mice, and sheep. During short-term inoculation studies in cats, the feline immunodeficiency-like syndrome found in nature was not experimentally induced, but a distinct primary phase of infection was observed. Fever and neutropenia were observed 4 to 5 weeks after inoculation; fever lasted several days, and neutropenia persisted from 1 to 9 weeks. Generalized lymphadenopathy that persisted for 2 to 9 months appeared at the same time. Antibodies to FIV appeared 2 weeks after inoculation and then plateaued. Virus was reisolated from the blood of all infected cats within 4 to 5 weeks after inoculation and persisted indefinitely in the face of humoral antibody response. Virus was recovered from blood, plasma, CSF and saliva, but not from colostrum or milk. Contact transmission was achieved slowly in one colony of naturally infected cats, but not between experimentally infected and susceptible specific-pathogen-free cats kept together for periods as long as 4 to 14 months. The infection was transmitted readily, however, by parenteral inoculation with blood, plasma, or infective cell culture fluids. In utero and lactogenic transmission were not observed in kittens born to naturally or experimentally infected queens. Lymphadenopathy observed during the initial stage of FIV infection was ascribed to lymphoid hyperplasia and follicular dysplasia. A myeloproliferative disorder was observed in 1 cat with experimentally induced infection.
On closely coupled dipoles in a random field (2006)

Reception of partially correlated fields by two closely coupled electrical dipoles is discussed as a function of load impedances and open-circuit correlations. Two local maxima of the power may be achieved for two different load impedances, but in those cases the output correlations are high. Choosing the loads to give zero output correlations is possible for almost any real-valued open-circuit correlation with a resulting reduction of received power or gain. However, the excess loss is only of the order 1.5 $ dB$ for a spacing as small as 0.05 wavelength.
Alteration of Skin Wound Healing in Keratinocyte-Specific Mediator Complex Subunit 1 Null Mice (2014)

MED1 (Mediator complex subunit 1) is a co-activator of various transcription factors that function in multiple transcriptional pathways. We have already established keratinocyte-specific MED1 null mice (Med1 epi2/2 ) that develop epidermal hyperplasia. Herein, to investigate the function(s) of MED1 in skin wound healing, full-thickness skin wounds were generated in Med1 epi2/2 and age-matched wild-type mice and the healing process was analyzed. Macroscopic wound closure and the re-epithelialization rate were accelerated in 8-week-old Med1 epi2/2 mice compared with age-matched wildtype mice. Increased lengths of migrating epithelial tongues and numbers of Ki67-positive cells at the wounded epidermis were observed in 8-week-old Med1 epi2/2 mice, whereas wound contraction and the area of a-SMA-positive myofibroblasts in the granulation tissue were unaffected. Migration was enhanced in Med1 epi2/2 keratinocytes compared with wild-type keratinocytes in vitro. Immunoblotting revealed that the expression of follistatin was significantly decreased in Med1 epi2/2 keratinocytes. Moreover, the mitogen-activated protein kinase pathway was enhanced before and after treatment of Med1 epi2/2 keratinocytes with activin A in vitro. Cell-cycle analysis showed an increased ratio of S phase cells after activin A treatment of Med1 epi2/2 keratinocytes compared with wild-type keratinocytes. These findings indicate that the activinfollistatin system is involved in this acceleration of skin wound healing in 8-week-old Med1 epi2/2 mice. On the other hand, skin wound healing in 6-month-old Med1 epi2/2 mice was significantly delayed with decreased numbers of Ki67-positive cells at the wounded epidermis as well as BrdU-positive label retaining cells in hair follicles compared with age-matched wildtype mice. These results agree with our previous observation that hair follicle bulge stem cells are reduced in older Med1 epi2/2 mice, indicating a decreased contribution of hair follicle stem cells to epidermal regeneration after wounding in 6-month-old Med1 epi2/2 mice. This study sheds light on the novel function of MED1 in keratinocytes and suggests a possible new therapeutic approach for skin wound healing and aging.
Mitochondrial portraits of human populations using median networks. (1995)

Analysis of variation in the hypervariable region of mitochondrial DNA (mtDNA) has emerged as an important tool for studying human evolution and migration. However, attempts to reconstruct optimal intraspecific mtDNA phylogenies frequently fail because parallel mutation events partly obscure the true evolutionary pathways. This makes it inadvisable to present a single phylogenetic tree at the expense of neglecting equally acceptable ones. As an alternative, we propose a novel network approach for portraying mtDNA relationships. For small sample sizes (< approximately 50), an unmodified median network contains all most parsimonious trees, displays graphically the full information content of the sequence data, and can easily be generated by hand. For larger sample sizes, we reduce the complexity of the network by identifying parallelisms. This reduction procedure is guided by a compatibility argument and an additional source of phylogenetic information: the frequencies of the mitochondrial haplotypes. As a spin-off, our approach can also assist in identifying sequencing errors, which manifest themselves in implausible network substructures. We illustrate the advantages of our approach with several examples from existing data sets.
Quantitative analysis of protein synthesis inhibition and recovery in CRM107 immunotoxin-treated HeLa cells (1998)

Previously a mathematical model was proposed that quantitatively related protein synthesis inhibition kinetics of antitransferrin receptor-gelonin immunotoxins to the cellular trafficking of the targeting agent. That work is here extended to describe protein synthesis inhibition kinetics of immunotoxins containing the diphtheria toxin mutant CRM107. CRM107 differs from gelonin in both translocation and ribosomal inactivation mechanisms. Targeting agents used were antitransferrin monoclonal antibodies 5E9 and OKT9, OKT9Fab, and transferrin. CRM107 conjugates inhibited protein synthesis at substantially lower concentrations than gelonin conjugates; this effect was attributed to substantially higher translocation rates for CRM107. However, under certain conditions, CRM107 immunotoxin-treated cells were able to recover completely; this behavior was never observed with gelonin immunotoxins. To quantitatively capture this phenomenon, extracellular and cytosolic degradation of the toxin as well as growth-related recovery from toxin-induced damage were incorporated into the mathematical model. Translocation and cytosolic degradation rate constants were determined for each immunotoxin. Unlike the gelonin conjugates, the translocation rate of CRM107 conjugates depended on the targeting molecule. This provided indirect evidence that CRM107 remains disulfide linked to the targeting agent for at least part of the translocation process. Although the CRM107 conjugates all had higher translocation rates and inhibited protein synthesis at lower concentrations than the gelonin conjugates, the cells' ability to recover from protein synthesis inhibition at low immunotoxin concentrations limits the utility of CRM107 conjugates for targeted cell killing.
Expression in Escherichia coli of Seven DNA Fragments Comprising the Complete L1 and L2 Open Reading Frames of Human Papillomavirus Type 6b and Localization of the 'Common Antigen' Region (1989)

Molecular cloning was used to express human papillomavirus type 6b (HPV-6b) antigens in Escherichia coli. Seven genomic DNA fragments of HPV-6b which together comprise the complete L1 and L2 open reading frames, known to code for capsid proteins, were cloned and expressed in E. coli as both beta-galactosidase and TrpE fusion proteins. Western blots of HPV-6b beta-galactosidase fusion proteins using 'genus-specific' antisera produced by immunization of rabbits with disrupted bovine papillomavirus type 1 (BPV-1) showed that polypeptides encoded by two DNA fragments from the mid portion of L1 of HPV-6b were cross-reactive. Only one of these two polypeptides reacted with antisera raised against disrupted HPV-1, directly demonstrating that this polypeptide contains the papillomavirus 'common antigen'. The cross-reactive region was confirmed by reversing antigen and antibody. Polyclonal antisera were raised against the seven HPV-6b beta-galactosidase fusion proteins and tested against BPV-1 virion proteins on Western blots. Only antiserum against the mid portion of L1 of HPV-6b reacted with the BPV-1 major capsid protein. HPV-6b fusion proteins were also used to test human sera for antibodies reactive in Western blots. Serum samples from 38 patients with documented HPV-6 infections and from 22 presumably uninfected controls were tested. Antibodies were not detected in any of the sera to any of the seven fusion proteins. HPV-6b beta-galactosidase fusion proteins are antigenic and can be used on Western blots to localize immunologically reactive sub-regions of proteins by reacting protein fragments with antisera from immunized animals. However, alternative methods will be required to detect anti-HPV antibodies in human sera.
Solution- and Solid-Phase Synthesis of 4-Hydroxy-4,5-dihydroisoxazole Derivatives from Enantiomerically Pure N-Tosyl-2,3-aziridine Alcohols (2002)

[reaction: see text] Enantiomerically pure N-tosyl-2,3-aziridine alcohols are directly converted into 4-hydroxy-4,5-dihydroisoxazole 2-oxides through oxidation to the corresponding aldehydes followed by in situ tandem nitroaldol-intramolecular cyclization. This study was concerned with (i) the selection of a suitable aziridine activation, (ii) the preparation of the target 4-hydroxy-4,5-dihydroisoxazole derivatives in solution, and (iii) the elaboration of a solid-phase process using hydroxy Merrifield-supported nitroacetic acid ester.
Investigation of sex-hormone binding globulin interference in direct radioimmunoassays for testosterone and estradiol. (1989)

The aim of this study was to find reasons for the interference of sex-hormone binding globulin (SHBG) in direct radioimmunoassays (RIAs) for testosterone and estradiol. 125I-labeled testosterone and estradiol were compared with 3H-labeled testosterone and estradiol for their affinity to SHBG. The 3H-labeled steroids bound far more avidly to SHBG than did the corresponding 125I-labeled steroids. The SHBG concentration in the standard matrix of two kits was significant. Finally, 3H-labeled steroids were compared with 125I-labeled steroids for their affinity to the antibody supplied with the kits. The 125I-labeled steroids bound more avidly than did the 3H-labeled steroids to the corresponding antibodies. Direct testosterone and estradiol results would be influenced according to the SHBG in the sample, as observed.
Effect of sample dilutions on arterial blood gas determinations (1985)

A study was undertaken to determine the blood gas effects of incompletely purging heparinized saline flush solution from an indwelling arterial catheter and pressure tubing. Hematocrit and blood gases were measured after withdrawing 0, 2, 4, 6, 8, and 10 ml of flush-blood solutions before sampling from a 20-ga radial artery catheter and 7-ft pressure tubing and stopcock. The pH and hematocrit were nearly unchanged between purging volumes of 8 and 10 ml. The PaO2 had a 2.4% error, while the PaCO2 had a 4.4% error. Because there is no standard arterial line setup, it is recommended that each ICU undertake a similar study to determine the optimal volume of aspirated flush-blood solution before blood gas sampling, in order to achieve accurate blood gas results and minimize blood waste.
Heat shock protein 72 on tumor cells: a recognition structure for natural killer cells (1997)

Evidence is accumulating that members of the heat shock protein 70 (HSP70) family are found on the cell surface of certain tumor cells where they elicit a strong antitumor immune response. We demonstrated that HSP72, the major heat-inducible form of the HSP70 group, is located on the cell surface of approximately 60% of the human colon carcinoma cells CX2 with two different mAbs by indirect immunofluorescence, by electron microscopy, and by selective cell surface biotinylation. In an effort to analyze the role of HSP72 cell surface expression as a tumor-specific recognition structure within an "autologous" tumor system, the CX2 cells were separated into a stably HSP72 high expressing (CX+: >90%) and a stably HSP72 low expressing (CX-: <20%) subline. The expression "autologous" was written in parentheses to indicate that the colon carcinoma sublines CX+ and CX- derived from the original CX2 tumor cell line differ with respect to the cell surface expression pattern of HSP72, whereas they exhibit an identical cell surface expression pattern of MHC and cellular adhesion molecules (e.g., intercellular cellular adhesion molecule, neural cellular adhesion molecule, vascular cellular adhesion molecule). Within this "autologous" tumor cell system, we demonstrate that the sensitivity to lysis mediated by adherent non-MHC-restricted effector cells correlates (p < 0.05) with the amount of HSP72 that is expressed on the cell surface. Blocking studies using an HSP72-specific mAb revealed that HSP72 might act in an MHC-unrestricted manner as a tumor-specific recognition structure for a distinct NK cell population.
Compressive sensing with modified Total Variation minimization algorithm (2010)

In this paper, the reconstruction problem of compressive sensing algorithm that is exploited for image compression, is investigated. Considering the Total Variation (TV) minimization algorithm, and by adding some new constraints compatible with typical image properties, the performance of the reconstruction is improved. Using DCT and contourlet transforms, sparse expansion of the image are exploited to provide new constraints to remove irrelevant vectors from the feasible set of the optimization problem while keeping the problem as a standard Second Order Cone Programming (SOCP) one. Experimental results show that, the proposed method, with new constraints, outperforms the conventional TV minimization method by up to 2 dB in PSNR.
Macrophages express osteopontin during repair of myocardial necrosis. (1994)

Osteopontin is a secreted glycoprotein implicated in a variety of functions, including cell adhesion and migration. Because these functions may be of general importance in the response of tissue to injury, we examined osteopontin expression after experimental cardiac injury and human myocardial infarction. Rat hearts were injured by transdiaphragmatic freeze-thaw and examined from 1 to 28 days after injury. Osteopontin was absent from normal myocardium by immunocytochemistry, Western blotting, and in situ hybridization. On days 1 and 2 after injury, osteopontin mRNA and protein were expressed at high levels by macrophages infiltrating necrotic myocardium. Double labeling with the macrophage marker ED1, however, demonstrated that only a subset of macrophages expressed osteopontin. Western blot analysis showed a single 66-kd band in injured myocardium that was absent from control tissue. Although macrophages remained abundant in the ensuing granulation response and scar tissue formation, the expression of osteopontin was diminished on day 4 and markedly downregulated at 1 and 4 weeks after injury, with only rare cells expressing the message or protein. In a human heart with an 8-day-old myocardial infarct, there was abundant expression of osteopontin mRNA and protein in macrophages within the necrotic and granulation tissue. Transient expression of osteopontin was also observed in a subset of macrophages infiltrating lung, skin, and skeletal muscle injured during the experiment, indicating the response was not limited to the heart. Thus, synthesis of osteopontin by macrophages appears to be a generalized response in the reaction to tissue injury. Although macrophages persist in these lesions, osteopontin is dramatically downregulated as healing proceeds. These results provide the first evidence that osteopontin may be important in healing after tissue injury, possibly in cellular adhesion, chemotaxis, and/or phagocytosis.