gebhart/patrepeval huggingface dataset:
Acute Kidney Injury Results in Long-Term Diastolic Dysfunction That Is Prevented by Histone Deacetylase Inhibition (2021)

Growing epidemiological data demonstrate that acute kidney injury (AKI) is associated with long-term cardiovascular morbidity and mortality. Here, the authors present a 1-year study of cardiorenal outcomes following bilateral ischemia-reperfusion injury in male mice. These data suggest that AKI causes long-term dysfunction in the cardiac metabolome, which is associated with diastolic dysfunction and hypertension. Mice treated with the histone deacetylase inhibitor, ITF2357, had preservation of cardiac function and remained normotensive throughout the study. ITF2357 did not protect against the development of kidney fibrosis after AKI.
<title>Computational intelligence for target assessment in Parkinson's disease</title> (2001)

Recent advances in image and signal processing have created a new challenging environment for biomedical engineers. Methods that were developed for different fields are now finding a fertile ground in biomedicine, especially in the analysis of bio-signals and in the understanding of images. More and more, these methods are used in the operating room, helping surgeons, and in the physician's office as aids for diagnostic purposes. Neural Network (NN) research on the other hand, has gone a long way in the past decade. NNs now consist of many thousands of highly interconnected processing elements that can encode, store and recall relationships between different patterns by altering the weighting coefficients of inputs in a systematic way. Although they can generate reasonable outputs from unknown input patterns, and can tolerate a great deal of noise, they are very slow when run on a serial machine. We have used advanced signal processing and innovative image processing methods that are used along with computational intelligence for diagnostic purposes and as visualization aids inside and outside the operating room. Applications to be discussed include EEGs and field potentials in Parkinson's disease along with 3D reconstruction of MR or fMR brain images in Parkinson's patients, are currently used in the operating room for Pallidotomies and Deep Brain Stimulation (DBS).
Isolation and characterization of CD6- T cells from peripheral blood. (1994)

Antibodies to the CD6 Ag have been described as having pan-T cell reactivity. We have recently demonstrated, however, that after treatment of PBL with an anti-CD6-blocked ricin-conjugated immunotoxin, clonal populations of CD3+, CD6- cells can be identified. Herein we show that through dual parameter staining of freshly isolated E-rosette+ cells, an average of 5 to 6% of either CD3+ or CD5+ cells express little or no CD6 on their surface. After negative selection by antibody-coated paramagnetic bead depletion, expanded CD6- T cells were shown to be CD1a-, CD2+, CD3+, CD5+, CD16-, CD56-, TCR-gamma/delta-, and consisted of both CD4+ and CD8+ cells. Furthermore, staining of digitonin permeabilized cells showed no cytoplasmic expression of the CD6 Ag and CD6 mRNA was not detected by Northern blot analysis. Identical staining patterns were observed for T cell clones isolated through bead depletion or immunotoxin treatment and expanded with either PHA or immobilized anti-CD3 mAb. It was also found that, relative to unfractionated T cells, the surface expression of CD5 was significantly diminished on CD6- T cells. Functionally, freshly isolated CD6- T cells showed substantially reduced alloreactivity in MLR compared with unfractionated E-rosette+ cells, yet both gave similar proliferative responses to either PHA or soluble tetanus toxin Ag. We conclude that there exists a minor subpopulation of mature T cells in peripheral blood that lack CD6. The diminished alloreactivity of these cells may help to explain the low incidence of graft-vs-host disease, despite high levels of engraftment, that has been reported in allogeneic bone marrow transplant patients receiving marrow treated with anti-CD6 (T12) mAb plus C'.
The glutamate analogue alpha-aminoadipic acid is taken up by astrocytes before exerting its gliotoxic effect in vitro (1984)

DL- and L-alpha-aminoadipic acid (alpha-AA) are specific gliotoxins in vitro (Huck, S., F. Grass, and M. E. Hatten (1984) Neuroscience 12: 783-796). By combining immunohistochemical and autoradiographic techniques, we now show that DL-[14C]-alpha-AA is accumulated almost selectively by astrocytes in cultures of the dissociated postnatal mouse cerebellum, presumptive neurons being free of the radiolabel. High pressure liquid chromatography analysis of cultures incubated with D- or L-alpha-AA and DL-[14C]-alpha-AA autoradiograms conducted in the presence of D- or L-alpha-AA reveal a stereospecificity of astroglial L-alpha-AA uptake. Both the uptake of alpha-AA by astrocytes and alpha-AA-induced gliotoxicity were sodium dependent. Since 2 microM tetrodotoxin did not prevent the morphological changes, we conclude that sodium plays its role in alpha-AA-induced gliotoxicity by mediating the transport of the substance. Thus, alpha-AA appears to be taken up by the astrocytes before exerting its cytotoxic effect.
Facile Isolation of Adsorbent-Free Long and Highly-Pure Chirality-Selected Semiconducting Single-Walled Carbon Nanotubes Using A Hydrogen-bonding Supramolecular Polymer (2015)

The ideal form of semiconducting-single-walled carbon nanotubes (sem-SWNTs) for science and technology is long, defect-free, chirality pure and chemically pure isolated narrow diameter tubes. While various techniques to solubilize and purify sem-SWNTs have been developed, many of them targeted only the chiral- or chemically-purity while sacrificing the sem-SWNT intrinsic structural identities by applying strong ultra-sonication and/or chemical modifications. Toward the ultimate purification of the sem-SWNTs, here we report a mild-conditioned extraction of the sem-SWNTs using removable supramolecular hydrogen-bonding polymers (HBPs) that are composed of dicarboxylic- or diaminopyridyl-fluorenes with ~70%-(8,6)SWNT selective extraction. Replacing conventional strong sonication techniques by a simple shaking using HPBs was found to provide long sem-SWNTs (>2.0 μm) with a very high D/G ratio, which was determined by atomic force microscopy observations. The HBPs were readily removed from the nanotube surfaces by an outer stimulus, such as a change in the solvent polarities, to provide chemically pure (8,6)-enriched sem-SWNTs. We also describe molecular mechanics calculations to propose possible structures for the HBP-wrapped sem-SWNTs, furthermore, the mechanism of the chiral selectivity for the sorted sem-SWNTs is well explained by the relationship between the molecular surface area and mass of the HBP/SWNT composites.
Experimental Autoimmune Uveoretinitis in the Rat and Mouse (2003)

Experimental autoimmune uveoretinitis (EAU) in rats and mice is a prototypic T cell-mediated autoimmune disease that targets the neural retina and related tissues. The model is used to represent human sight-threatening inflammatory eye diseases that are believed to have an autoimmune etiology, and to study basic mechanisms of tolerance and autoimmunity to organ-specific antigens from immunologically privileged sites. In this unit, EAU is induced in rats and mice by immunization with uveitogenic peptide antigens emulsified in complete Freund's adjuvant (CFA). Clinical onset is observed by both external and microscopic examination. A protocol is provided for preparation of tissue sections of affected eyes for microscopic analysis. EAU can also be induced in the rat (as described) by adoptive transfer of lymphocytes from uveitic rats into unimmunized recipients, which obviates the use of CFA. To induce EAU in mice, Bordetella pertussis toxin (PTX) is included to overcome immunological resistance mechanisms.
Auditory Cortical Neurons Respond to Somatosensory Stimulation (2003)

The prevailing hierarchical model of cortical sensory processing holds that early processing is specific to individual modalities and that combination of information from different modalities is deferred until higher-order stages of processing. In this paper, we present physiological evidence of multisensory convergence at an early stage of cortical auditory processing. We used multi-neuron cluster recordings, along with a limited sample of single-unit recordings, to determine whether neurons in the macaque auditory cortex respond to cutaneous stimulation. We found coextensive cutaneous and auditory responses in caudomedial auditory cortex, an area lying adjacent to A1, and at the second stage of the auditory cortical hierarchy. Somatosensory-auditory convergence in auditory cortex may underlie effects observed in human studies. Convergence of inputs from different sensory modalities at very early stages of cortical sensory processing has important implications for both our developing understanding of multisensory processing and established views of unisensory processing.
The Use of Monoclonal Antibodies to Localize the Low Density Lipoprotein Receptor-binding Domain of Apolipoprotein B (1989)

Human apolipoprotein (apo) B-100 is composed of 4536 amino acids. It is thought that the binding of apoB to the low density lipoprotein (LDL) receptor involves an interaction between basic amino acids of the ligand and acidic residues of the receptor. Three alternative models have been proposed to describe this interaction: 1) a single region of apoB is involved in receptor binding; 2) groups of basic amino acids from throughout the apoB primary structure act in concert in apoB receptor binding; and 3) apoB contains multiple independent binding regions. We have found that monoclonal antibodies (Mabs) specific for a region that spans a thrombin cleavage site at apoB residue 3249 (T2/T3 junction) totally blocked LDL binding to the LDL receptor. Mabs specific for epitopes outside this region had either no or partial ability to block LDL binding. In order to define the region of apoB directly involved in the interaction with the LDL receptor we have tested 22 different Mabs for their ability to bind to LDL already fixed to the receptor. A Mab specific for an epitope situated between residues 2835 and 2922 could bind to its epitope on LDL fixed to its receptor whereas a second epitope between residues 2980 and 3084 is inaccessible on receptor-bound LDL. A series of epitopes near residue 3500 of apoB is totally inaccessible, and another situated between residues 4027 and 4081 is poorly accessible on receptor-bound LDL. In contrast, an epitope that is situated between residues 4154 and 4189 is fully exposed. Mabs specific for epitopes upstream and downstream of the region 3000-4000 can bind to receptor-bound LDL with a stoichiometry close to unity. Our results strongly suggest that the unique region of apoB directly involved in the LDL-receptor interaction is that of the T2/T3 junction.
Delivering Type I Interferon to Dendritic Cells Empowers Tumor Eradication and Immune Combination Treatments (2017)

An ideal generic cancer immunotherapy should mobilize the immune system to destroy tumor cells without harming healthy cells and remain active in case of recurrence. Furthermore, it should preferably not rely on tumor-specific surface markers, as these are only available in a limited set of malignancies. Despite approval for treatment of various cancers, clinical application of cytokines is still impeded by their multiple toxic side effects. Type I IFN has a long history in the treatment of cancer, but its multifaceted activity pattern and complex side effects prevent its clinical use. Here we develop AcTakines (Activity-on-Target cytokines), optimized (mutated) immunocytokines that are up to 1,000-fold more potent on target cells, allowing specific signaling in selected cell types only. Type I IFN-derived AcTaferon (AFN)-targeting Clec9A<sup>+</sup> dendritic cells (DC) displayed strong antitumor activity in murine melanoma, breast carcinoma, and lymphoma models and against human lymphoma in humanized mice without any detectable toxic side effects. Combined with immune checkpoint blockade, chemotherapy, or low-dose TNF, complete tumor regression and long-lasting tumor immunity were observed, still without adverse effects. Our findings indicate that DC-targeted AFNs provide a novel class of highly efficient, safe, and broad-spectrum off-the-shelf cancer immunotherapeutics with no need for a tumor marker.<b>Significance:</b> Targeted type I interferon elicits powerful antitumor efficacy, similar to wild-type IFN, but without any toxic side effects. <i>Cancer Res; 78(2); 463-74. ©2017 AACR</i>.
Role of Supply Chain Practices on Customer Satisfaction in the Printing Industry in Kenya: A Case Study of Morven Kester East Africa Limited (2014)

The purpose of this study was to carry out an investigation on the role of supply chain practices on customer satisfaction in the printing industry. Studies by previous researchers have indicated that printing industry faces the challenges of uncertainty associated with sourcing for raw materials from oversees and failure to embrace modern technology which adversely affected the ability to satisfy customers.
Heart Failure: Pathophysiology, Molecular Biology, and Clinical Management (2000)

Preface Overview, Definition, Historical Aspects Organ Physiology:The Failing Heart as a Weakened Pump Cell Biochemistry:Abnormal Excitation-Contraction Coupling,Contraction, and Relaxation Arrhythmias Energy Starvation The Hemodynamic Defense Reaction Neurohumoral Response:The Inflammatory Response The Hypertrophic Response:Programmed Cell Death Signal Transduction Within Cells of the Failing Heart Maladaptive Hypertrophy and the Cardiomyopathy of Overload:Familial Cardiomyopathies Therapeutic Strategies for Managing Heart Failure Care of the Heart Failure Patient Inde
Comparative genomic analysis of carbon and nitrogen assimilation mechanisms in three indigenous bioleaching bacteria: predictions and validations (2008)

The current study utilized genomic and molecular evidence to verify carbon and nitrogen fixation mechanisms for three bioleaching bacteria and provided an analysis of the potential regulatory pathways and functional networks that control carbon and nitrogen fixation in these microorganisms.
Intracellular Antibodies as a New Class of Therapeutic Molecules for Gene Therapy (1994)

Intracellularly expressed antibodies, referred to as "intrabodies" can be designed to bind and inactivate target molecules inside cells. In our previous study, mammalian cells were transduced to produce an anti-gp120 single-chain intrabody sFv105 to inactivate human immunodeficiency virus type-1 (HIV-1) infection. Here, an inducible expression vector was constructed in which the sFv105 intrabody, which reacts with the CD4-binding site of HIV-1 gp120, is under the control of the HIV-1 long terminal repeat (LTR)/promoter. The sFv105 intrabody is inducibly expressed after HIV-1 infection or in the presence of Tat protein and is retained intracellularly. A human CD4+ lymphocyte line transformed with the expression vector exhibits resistance to the virus-mediated syncytium formation and a decreased ability to support HIV-1 production. Surface gp120 expression is markedly reduced and surface CD4 is restored to normal following HIV-1 infection in the transformed lymphocytes. Cell-surface phenotype, replication rate, morphology, and response to mitogenic stimulation of the transformed cells are also normal. Thus, intrabodies are a new class of active molecules that may be useful for the gene therapy of acquired immunodeficiency virus (AIDS) and other diseases.
STAPHYLOCOCCUS AUREUS PNEUMONIA (1919)

In the standard textbooks the staphylococcus is usually noted among other micro-organisms as a possible etiologic agent in the production of bronchopneumonia. A detailed description of the clinical picture of the disease associated with the presence of this organism in the lung has thus far failed to come to our attention. This is no doubt due in part to the relative rarity of this infection as encountered in civil practice, and possibly to the lack of careful study of the bacteriology of acute respiratory affections until but recently. How rarely<i>Staphylococcus aureus</i>infection of the lung occurs and how grave the prognosis is may best be stated by noting that in a series of about 800 cases of pneumonia, drawn from all classes of the population of New York City, only thirteen cases were treated at the hospital of the Rockefeller Institute under the direction of Dr. Rufus Cole, in
Linear pushbroom cameras (1997)

Modeling and analyzing pushbroom sensors commonly used in satellite imagery is difficult and computationally intensive due to the motion of an orbiting satellite with respect to the rotating Earth, and the nonlinearity of the mathematical model involving orbital dynamics. In this paper, a simplified model of a pushbroom sensor (the linear pushbroom model) is introduced. It has the advantage of computational simplicity while at the same time giving very accurate results compared with the full orbiting pushbroom model. Besides remote sensing, the linear pushbroom model is also useful in many other imaging applications. Simple noniterative methods are given for solving the major standard photogrammetric problems for the linear pushbroom model: computation of the model parameters from ground-control points; determination of relative model parameters from image correspondences between two images; and scene reconstruction given image correspondences and ground-control points. The linear pushbroom model leads to theoretical insights that are approximately valid for the full model as well. The epipolar geometry of linear pushbroom cameras is investigated and shown to be totally different from that of a perspective camera. Nevertheless, a matrix analogous to the fundamental matrix of perspective cameras is shown to exist for linear pushbroom sensors. From this it is shown that a scene is determined up to an affine transformation from two views with linear pushbroom cameras.
Apparent molal heat capacities and volumes of aqueous electrolytes at 25 °C: NaClO3, NaClO4, NaNO3, NaBrO3, NaIO3, KClO3, KBrO3, KIO3, NH4NO3, NH4Cl, and NH4ClO4 (1978)

Measurements at 25 °C with flow calorimeters and densimeters have led to heat capacities and densities of aqueous solutions of 11 1:1 electrolytes: NaClO 3 , NaBrO 3 , NaIO 3 , NaNO 3 , NaClO 4 , NH 4 NO 3 , KClO 3 , KBrO 3 , KIO 3 , NH 4 Cl, and NH 4 ClO 4 . The first 6 salts were studied up to near saturation. We have used results of these measurements to obtain apparent molal heat capacities and apparent molal volumes of the various solutes. Extrapolation to infinite dilution on the basis of the Debye–Hückel theory bas led to [Formula: see text]and [Formula: see text] values for each solute. We have compared these standard values with results of earlier investigations.
The promyelocytic leukemia zinc finger (PLZF) protein binds DNA in a high molecular weight complex associated with cdc2 kinase (1999)

A binding site selection from a CpG island library for the promyelocytic leukemia zinc finger protein (PLZF) identified two high affinity PLZF binding sites. These sequences also bound RARalpha/PLZF, a fusion protein formed in chromosomal translocation t(11;17)(q23;q21) associated with acute promyelocytic leukemia. PLZF bound DNA as a slowly migrating complex with an estimated mol. wt of 600 kDa whose formation was dependent on the POZ/dimerization domain of PLZF. The PLZF-DNA complex was unable to form in the presence of cdc2 antibodies. A PLZF-cdc2 interaction was further demonstrated by co-immunoprecipitation and a biotin-streptavidin pull-down assay. PLZF is a phosphoprotein and immunoprecipi-tates with a cdc2-like kinase activity. The PLZF-DNA complex was abolished with the addition of a phosphatase. These studies suggest that the activity of PLZF, a regulator of the cell cycle, may be modulated by cell cycle proteins. RARalpha/PLZF did not complex with cdc2, this potentially contributing to its aberrant transcriptional properties and potential role in leukemo-genesis.
Highly multiplexed signal readout for a time-of-flight positron emission tomography detector based on silicon photomultipliers (2017)

Maintaining excellent timing resolution in the generation of silicon photomultiplier (SiPM)-based time-of-flight positron emission tomography (TOF-PET) systems requires a large number of high-speed, high-bandwidth electronic channels and components. To minimize the cost and complexity of a system's back-end architecture and data acquisition, many analog signals are often multiplexed to fewer channels using techniques that encode timing, energy, and position information. With progress in the development SiPMs having lower dark noise, after pulsing, and cross talk along with higher photodetection efficiency, a coincidence timing resolution (CTR) well below 200 ps FWHM is now easily achievable in single pixel, bench-top setups using 20-mm length, lutetium-based inorganic scintillators. However, multiplexing the output of many SiPMs to a single channel will significantly degrade CTR without appropriate signal processing. We test the performance of a PET detector readout concept that multiplexes 16 SiPMs to two channels. One channel provides timing information with fast comparators, and the second channel encodes both position and energy information in a time-over-threshold-based pulse sequence. This multiplexing readout concept was constructed with discrete components to process signals from a [Formula: see text] array of SensL MicroFC-30035 SiPMs coupled to [Formula: see text] Lu<sub>1.8</sub>Gd<sub>0.2</sub>SiO<sub>5</sub> (LGSO):Ce (0.025 mol. %) scintillators. This readout method yielded a calibrated, global energy resolution of 15.3% FWHM at 511 keV with a CTR of [Formula: see text] FWHM between the 16-pixel multiplexed detector array and a [Formula: see text] LGSO-SiPM reference detector. In summary, results indicate this multiplexing scheme is a scalable readout technique that provides excellent coincidence timing performance.
Potential Anticancer Agents. II. Urethan-Type Nitrogen Mustards of Some Natural Sex Hormones (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotential Anticancer Agents. II. Urethan-Type Nitrogen Mustards of Some Natural Sex HormonesI. Niculescu-Duvaz, A. Cambanis, and E. TárnáuceanuCite this: J. Med. Chem. 1967, 10, 2, 172–174Publication Date (Print):March 1, 1967Publication History Published online1 May 2002Published inissue 1 March 1967https://pubs.acs.org/doi/10.1021/jm00314a009https://doi.org/10.1021/jm00314a009research-articleACS PublicationsRequest reuse permissionsArticle Views113Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts