gebhart/patrepeval huggingface dataset:
El papel de la gran propiedad en el proceso de constricción de la ciudad europea durante el último tercio del siglo xx. Las propiedades militares como referente /the role of great property in the european city-making process in the last third ot the 20th century. Military property as reference (2020)

The thesis concerns a work of urban history intended not to describe the city but rather to interpret it. By doing so, I have interpreted the city by means of the role played by the so-called ‘great property’ in the European city-making process during the last three decades of the 20th century, specifically focused on the concrete case of military properties in Italy. I have also considered the role played by other kinds of great properties, i.e. industries and railway, which previously acted in the production of the built environment in a different way respect to the military one. As all of them have as common denominator the fact of being ‘capital in land’, I analysed great industrial and railway properties in order to extrapolate a methodology which helped me to interpret the relationship between military properties and city-making process in Europe in the late 20th century.
 I have analysed the relationship between the capital in land and the city-making process on the ground of the understanding the interrelation between the great property, the urban development, and the agents involved in the urban and territorial planning. Here I have showed that urban planning is not the decisive factor influencing the city-making process, but instead the power held by the capital in land. I have found that is the great property the trigger of the creation of new ‘areas of centrality’ intended as large areas for consumerism. As far as the role played by great property is concerned, I have also discovered that it has evolved over time. Originally, industrial and railway properties have been regenerated into a wide range of new profit-driven spaces; successively, I have found out that most of the regeneration of military premises aimed to materialise areas of centrality. The way of interpreting this factor has been based on focusing my attention on the military premises in Italy: I have classified their typology when they have been built and, most importantly, when they have been regenerated into new areas of centrality.
Chemokine Receptor Expression Identifies Pre–T Helper (Th)1, Pre–Th2, and Nonpolarized Cells among Human CD4+ Central Memory T Cells (2004)

We previously reported that central-memory T cells (T(CM) cells), which express lymph node homing receptors CCR7 and CD62L, are largely devoid of effector functions but acquire characteristics of effector-memory T cells (T(EM) cells) (i.e., CCR7(-) T helper [Th]1 or Th2 cells) after stimulation with T cell receptor agonists or homeostatic cytokines. Here we show that three chemokine receptors identify functional subsets within the human CD4(+) T(CM) cell pool. T(CM) cells expressing CXCR3 secreted low amounts of interferon gamma, whereas CCR4(+) T(CM) cells produced some interleukin (IL)-4, but not IL-5. In response to IL-7 and IL-15, CXCR3(+) T(CM) and CCR4(+) T(CM) cells invariably generated fully differentiated CCR7(-) Th1 and Th2 cells, respectively, suggesting that they represent pre-Th1 and pre-Th2 cells. Conversely, CXCR5(+) T(CM) cells lacking CXCR3 and CCR4 remained nonpolarized and retained CCR7 and CD62L expression upon cytokine-driven expansion. Unlike naive cells, all memory subsets had a low T cell receptor rearrangement excision circle content, spontaneously incorporated bromodeoxyuridine ex vivo, and contained cells specific for tetanus toxoid. Conversely, recall responses to cytomegalovirus and vaccinia virus were largely restricted to CXCR3(+) T(CM) and T(EM) cells. We conclude that antigen-specific memory T cells are distributed between T(EM) cells and different subsets of T(CM) cells. Our results also explain how the quality of primary T cell responses could be maintained by T(CM) cells in the absence of antigen.
Generating Intestinal Tissue from Stem Cells: Potential for Research and Therapy (2011)

Intestinal resection and malformations in adult and pediatric patients result in devastating consequences. Unfortunately, allogeneic transplantation of intestinal tissue into patients has not been met with the same measure of success as the transplantation of other organs. Attempts to engineer intestinal tissue in vitro include disaggregation of adult rat intestine into subunits called organoids, harvesting native adult stem cells from mouse intestine and spontaneous generation of intestinal tissue from embryoid bodies. Recently, by utilizing principles gained from the study of developmental biology, human pluripotent stem cells have been demonstrated to be capable of directed differentiation into intestinal tissue in vitro. Pluripotent stem cells offer a unique and promising means to generate intestinal tissue for the purposes of modeling intestinal disease, understanding embryonic development and providing a source of material for therapeutic transplantation.
Sugar Residues on Protein (1981)

Glycoproteins have become increasingly important in the structure and function of many different mammalian systems; for example, membrane glycoproteins and glycoprotein hormones. It is, therefore, important to understand their chemistry, which would include an understanding of both the carbohydrate and protein parts of the molecule. Since the chemical characterization of the protein moiety has been extensively examined and the techniques for its characterization are well worked out, only the carbohydrate portion of glycoproteins will be reviewed in this article. The chemical nature of the carbohydrate moiety of glycoproteins will be examined. First, the types of monosaccharides present in animal systems, especially those in the mammalian systems, will be described. Next, various types of simple and complex carbohydrate chains will be discussed to establish the diversity, size, and number of chains present in the carbohydrate units in different glycoproteins. Then, the type of linkages of the carbohydrate to the protein will be examined to determine if the primary sequence of protein is important in determining the size and type of carbohydrate chains present in glycoproteins. Finally, the current methods of structural elucidation such as monosaccharide sequence, intersugar bonds, and anomeric linkages in the carbohydrate moiety of glycoproteins will be reviewed. These methods include the techniques of periodate oxidation, methylation, partial acid hydrolysis, and specific glycosidase digestion of glycoproteins, as well as the latest techniques using micromethods of carbohydrate quantitation and characterization involving gas chromatography and mass spectrometry. The function of the carbohydrate in glycoproteins will also be considered. First, hormone glycoproteins will be discussed in their relationship to the immunological and biological function of the glycoprotein when the carbohydrate is sequentially removed. Next, the function of the carbohydrate in the turnover of glycoproteins will be discussed. These topics will be considered in order to develop an understanding of a specific function(s) of the carbohydrate in glycoproteins.
Fish Oil Supplementation and Risk of Ventricular Tachycardia and Ventricular Fibrillation in Patients With Implantable Defibrillators (2005)

Among patients with a recent episode of sustained ventricular arrhythmia and an ICD, fish oil supplementation does not reduce the risk of VT/VF and may be proarrhythmic in some patients.
Molecular Properties That Influence the Oral Bioavailability of Drug Candidates (2002)

Oral bioavailability measurements in rats for over 1100 drug candidates studied at SmithKline Beecham Pharmaceuticals (now GlaxoSmithKline) have allowed us to analyze the relative importance of molecular properties considered to influence that drug property. Reduced molecular flexibility, as measured by the number of rotatable bonds, and low polar surface area or total hydrogen bond count (sum of donors and acceptors) are found to be important predictors of good oral bioavailability, independent of molecular weight. That on average both the number of rotatable bonds and polar surface area or hydrogen bond count tend to increase with molecular weight may in part explain the success of the molecular weight parameter in predicting oral bioavailability. The commonly applied molecular weight cutoff at 500 does not itself significantly separate compounds with poor oral bioavailability from those with acceptable values in this extensive data set. Our observations suggest that compounds which meet only the two criteria of (1) 10 or fewer rotatable bonds and (2) polar surface area equal to or less than 140 A(2) (or 12 or fewer H-bond donors and acceptors) will have a high probability of good oral bioavailability in the rat. Data sets for the artificial membrane permeation rate and for clearance in the rat were also examined. Reduced polar surface area correlates better with increased permeation rate than does lipophilicity (C log P), and increased rotatable bond count has a negative effect on the permeation rate. A threshold permeation rate is a prerequisite of oral bioavailability. The rotatable bond count does not correlate with the data examined here for the in vivo clearance rate in the rat.
Quantitative Imaging of Protein Interactions in the Cell Nucleus (2005)

Over the past decade, genetically encoded fluorescent proteins have become widely used as noninvasive markers in living cells. The development of fluorescent proteins, coupled with advances in digital imaging, has led to the rapid evolution of live-cell imaging methods. These approaches are being applied to address biological questions of the recruitment, co-localization, and interactions of specific proteins within particular subcellular compartments. In the wake of this rapid progress, however, come important issues associated with the acquisition and analysis of ever larger and more complex digital imaging data sets. Using protein localization in the mammalian cell nucleus as an example, we will review some recent developments in the application of quantitative imaging to analyze subcellular distribution and co-localization of proteins in populations of living cells. In this report, we review the principles of acquiring fluorescence resonance energy transfer (FRET) microscopy measurements to define the spatial relationships between proteins. We then discuss how fluorescence lifetime imaging microscopy (FLIM) provides a method that is independent of intensity-based measurements to detect localized protein interactions with spatial resolution. Finally, we consider potential problems associated with the expression of proteins fused to fluorescent proteins for FRET-based measurements from living cells.
Learning to Teach, Teaching to Learn (1997)

The Schreyer Institute for Teaching
Delivery of Antifibroblast Agents as Adjuncts to Filtration Surgery. Part I - Periocular Clearance of Cobalt-57 Bleomycin in Experimental Drug Delivery: Pilot Study in the Rabbit (1986)

Antitumor and antifibroblast agents show promise as adjuncts after glaucoma filtration surgery in reducing postoperative scarring and failure. We used nuclear imaging in rabbits to investigate periocular clearance of one such agent (57Co-bleomycin). Sub-Tenon injection was compared to other delivery techniques. Our results showed that a collagen sponge and a silastic disc implant with a microhole prolonged drug delivery when compared to sub-Tenon injection alone or injection with a viscosity enhancing agent (0.5% sodium hyaluronate). We theorize that if an antifibroblast agent can be delivered in small and sustained amounts after filtration surgery, this may prolong bleb longevity and avoid unnecessary drug toxicity.
Roles of Sema4D–Plexin-B1 Interactions in the Central Nervous System for Pathogenesis of Experimental Autoimmune Encephalomyelitis (2009)

Although semaphorins were originally identified as axonal guidance molecules during neuronal development, it is emerging that several semaphorins play crucial roles in various phases of immune responses. Sema4D/CD100, a class IV semaphorin, has been shown to be involved in the nervous and immune systems through its receptors plexin-B1 and CD72, respectively. However, the involvement of Sema4D in neuroinflammation still remains unclear. We found that Sema4D promoted inducible NO synthase expression by primary mouse microglia, the effects of which were abolished in plexin-B1-deficient but not in CD72-deficient microglia. In addition, during the development of experimental autoimmune encephalomyelitis (EAE), which was induced by immunization with myelin oligodendrocyte glycoprotein-derived peptides, we observed that the expression of Sema4D and plexin-B1 was induced in infiltrating mononuclear cells and microglia, respectively. Consistent with these expression profiles, when myelin oligodendrocyte glycoprotein-specific T cells derived from wild-type mice were adoptively transferred into plexin-B1-deficient mice or bone marrow chimera mice with plexin-B1-deficient CNS resident cells, the development of EAE was considerably attenuated. Furthermore, blocking Abs against Sema4D significantly inhibited neuroinflammation during EAE development. Collectively, our findings demonstrate the role of Sema4D-plexin-B1 interactions in the activation of microglia and provide their pathologic significance in neuroinflammation.
Circadian Profile of Systemic Hemodynamics (1995)

We determined the continuous 24-hour profile of mean arterial pressure, heart rate, stroke volume, cardiac output, and total peripheral resistance in eight healthy ambulatory volunteers. Beat-to-beat intra-arterial blood pressure was recorded with the Oxford system; subjects were ambulant during daytime and slept at night. Beat-to-beat stroke volume was determined by the pulse contour method from the arterial pulse wave. During the nighttime, compared with the daytime average, there was a decrease in blood pressure (9 mm Hg), heart rate (18 beats per minute), and cardiac output (29%); stroke volume showed a small decrease (7%), and total peripheral resistance increased unexpectedly by 22%. When subjects arose in the morning a steep increase in cardiac output and decrease in total peripheral resistance were found. Comparable changes were seen during a period of supine resting in the afternoon, whereas physical exercise caused opposite changes in hemodynamics. This pattern was observed in all subjects. We conclude that the circadian pattern of cardiac output and total peripheral resistance originates from the day-night pattern in physical activity: during the nighttime, blood flow to the skeletal muscles is decreased through local autoregulation, which increases total peripheral resistance and decreases cardiac output compared with the daytime.
ENDOTHELIAL NITRIC OXIDE SYNTHASE (2001)

In endothelium-dependent vasodilation, L-arginine is converted to citrulline, with the simultaneous release of nitric oxide (NO), a vasodilator. This reaction is catalyzed by nitric oxide synthase (NOS). Exercise training enhances endothelium-dependent vasodilation, presumably by increasing shear stress in a chronic but episodic manner. Hyperthyroidism has a similar but continuous effect. We hypothesized that NOS activity in the arota increases in exercise-trained and hyperthyroid rats. Rats were assigned to one of three groups: control (CO, n = 5), exercise-trained (TR, n = 5), or hyperthyroid (HY, n = 6). TR rats ran on a treadmill at 30 m/min, 5 d/week for 11–15 weeks. HY rats were injected every second day with T3 (300 ug/kg) for 17–20 weeks. Aortas were excised and endothelial proteins, including NOS, were extracted. NOS activity was determined using a 3H[L-arginine] to 3H[citrulline] assay. Left ventricular wt/body wt ratio was greater in HY (CO, 2.08 ± 0.22 mg/g; TR, 2.02 ± 0.04; HY, 2.59 ± 0.10; P < 0.05), as was resting oxygen consumption (20.84 ± 1.38 ml/min/kg for HY, 10.53 ± 0.38 for CO; P < 0.05), indicating treatment efficacy. Citrate synthase activity indicated significantly increased oxidative capacity in the soleus muscle (CO = 21.5 ± 2.1 umol/min/g, TR = 32.2 ± 1.7, HY = 37.3 ± 1.4; P < 0.05, TR & HY vs. CO), another indication of T3 and training efficacy. There were not any significant differences in aortic NOS activity among groups (CO = 65 ± 14 counts/hour/ug protein, TR = 57 ± 20, HY = 127 ± 21), although HY tended (0.05 < P < 0.10) to be greater than CO. We conclude that the increase in endothelium-dependent vasodilation associated with training and hyperthyroidism is not due to increased NOS activity. Supported by NIH HL 57226, AHA-KS-98-GB-25, and a Howard Hughes Medical Institute Undergraduate Biology Education Program Grant.
Infection and Vaccine-Induced Neutralizing-Antibody Responses to the SARS-CoV-2 B.1.617 Variants (2021)

To assess infection and vaccine-Induced Neutralizing-Antibody activity against the SARS-CoV-2 B.1.617.1 (or kappa) and B.1.617.2 (or delta) variants.
Expression and enzyme activity of glutathione reductase is upregulated by Fe-deficiency in graminaceous plants (2007)

Glutathione reductase (GR) plays an important role in the response to biotic and abiotic stresses in plants. We studied the expression patterns and enzyme activities of GR in graminaceous plants under Fe-sufficient and Fe-deficient conditions by isolating cDNA clones for chloroplastic GR (HvGR1) and cytosolic GR (HvGR2) from barley. We found that the sequences of GR1 and GR2 were highly conserved in graminaceous plants. Based on their nucleotide sequences, HvGR1 and HvGR2 were predicted to encode polypeptides of 550 and 497 amino acids, respectively. Both proteins showed in vitro GR activity, and the specific activity for HvGR1 was 3-fold that of HvGR2. Northern blot analyses were performed to examine the expression patterns of GR1 and GR2 in rice (Os), wheat (Ta), barley (Hv), and maize (Zm). HvGR1, HvGR2, and TaGR2 were upregulated in response to Fe-deficiency. Moreover, HvGR1 and TaGR1 were mainly expressed in shoot tissues, whereas HvGR2 and TaGR2 were primarily observed in root tissues. The GR activity increased in roots of barley, wheat, and maize and shoot tissues of rice, barley, and maize in response to Fe-deficiency. Furthermore, it appeared that GR was not post-transcriptionally regulated, at least in rice, wheat, and barley. These results suggest that GR may play a role in the Fe-deficiency response in graminaceous plants.
Inhibitory Effects of Tea Catechins, Black Tea Extract and Oolong Tea Extract on Hepatocarcinogenesis in Rat (1996)

Inhibitory effects of individual tea catechins ((--)-epicatechin, (--)-epigallocatechin, (--)-epicatechin gallate, (--)-epigallocatechin gallate), black tea extract and oolong tea extract on hepatocarcinogenesis were investigated. Male F344 rats received a single dose of diethylnitrosamine (200 mg/kg, i.p.), and thereafter phenobarbital (0.05%) was administered in the drinking water for a period of 6 weeks. Tea catechins, black tea extract or oolong tea extract were given during the entire experimental period, during only the initiation period or during only the promotion period. All four tea catechins, black tea extract and oolong tea extract (0.05 or 0.1%) significantly decreased the number and area of preneoplastic glutathione S-transferase placental form-positive foci in the liver. These results suggest that tea catechins, black tea extract and oolong tea extract have a chemopreventive action against hepatocarcinogenesis.
PV balancers: Concept, architectures, and realization (2012)

This paper presents a new concept of module-integrated converters called PV balancers for photovoltaic applications. The proposed concept enables independent maximum power point tracking (MPPT) for each module, and dramatically decreases the requirements for power converters. The power rating of a PV balancer is less than 20% of its counterparts, and the manufacturing cost is thus significantly reduced. In this paper, two architectures of PV balancers are proposed, analyzed, realized, and verified through simulation and experimental results. It is anticipated that the proposed approach will be a low-cost solution for future photovoltaic power systems.
Differential Regulation of Sphingosine-1-Phosphate- and VEGF-Induced Endothelial Cell Chemotaxis: Involvement of Gi α 2-Linked Rho Kinase Activity (2001)

We compared stimulus-coupling pathways involved in bovine pulmonary artery (PA) and lung microvascular endothelial cell migration evoked by sphingosine-1-phosphate (S1P), a potent bioactive lipid released from activated platelets, and by vascular endothelial growth factor (VEGF), a well-recognized angiogenic factor. S1P-induced endothelial cell migration was maximum at 1 microM (approximately 8-fold increase with PA endothelium) and surpassed the maximal response evoked by either VEGF (10 ng/ml) (approximately 2.5-fold increase) or hepatocyte growth factor (HGF) (approximately 2.5-fold increase). Migration induced by S1P, but not by VEGF, was significantly inhibited by treatment with antisense oligonucleotides directed to Edg-1 and Edg-3 (endothelial differentiation gene) S1P receptors and by G protein modification. These strategies included pretreatment with pertussis toxin, or transfection with mini-genes encoding a betagamma subunit inhibitory peptide of the beta-adrenergic receptor kinase, or an 11-amino-acid peptide that inhibits G(1alpha2) signaling. Various strategies to interrupt Rho family signaling, including C(3) exotoxin, dominant/negative Rho, or the addition of Y27632, a cell-permeable Rho kinase inhibitor, significantly attenuated S1P- but not VEGF-induced migration. Conversely, pharmacologic inhibition of either myosin light chain kinase, src family tyrosine kinases, or phosphatidylinositol-3' kinase reduced basal endothelial cell migration and abolished VEGF-induced endothelial cell migration but did not inhibit the increase in S1P-induced migration. Whereas VEGF and S1P increased both p42/p44 extracellular regulated kinase and p38 mitogen-activated protein (MAP) kinase activities, only p38 MAP kinase inhibition significantly reduced VEGF- and S1P-stimulated migration. These data confirm S1P as a potent endothelial cell chemoattractant through G(1alpha2)-coupled Edg receptors linked to Rho-associated kinase and p38 MAP kinase activation. The divergence in signaling pathways evoked by S1P and VEGF suggests complex and agonist-specific regulation of endothelial cell angiogenic responses.
TOOMAS: Interactive Shopping Guide robots in everyday use - final implementation and experiences from long-term field trials (2009)

The paper gives a comprehensive overview of our Shopping Guide project, which aims at the development of interactive mobile shopping companion robots for everyday use in challenging operating environments such as home improvement stores. It is spanning an arc from the expectations and requirements of store owners and customers, via the challenges of the shopping scenario and the operating environment, the implemented functionality of the shopping guide robots, up to the results of long-term field trials. The field trials started in April 2008 and still ongoing aim at studying whether and how a group of interactive mobile shopping guide robots can operate completely autonomously in such everyday environments and how they are accepted by uninstructed customers. In these field trials, where nine robotic shopping guides traveled together 2187 kilometers in three different home improvement stores in Germany, more than 8,600 customers were successfully guided to the locations of their products of choice. With the successful development of these shopping guide robots, a further important step towards assistive robotics for daily use has been done.