gebhart/patrepeval huggingface dataset:
Vascular Smooth Muscle Cells on Polyelectrolyte Multilayers:  Hydrophobicity-Directed Adhesion and Growth (2004)

Polyelectrolyte multilayer films were employed to support attachment of cultured rat aortic smooth muscle A7r5 cells. Like smooth muscle cells in vivo, cultured A7r5 cells are capable of converting between a nonmotile "contractile" phenotype and a motile "synthetic" phenotype. Polyelectrolyte films were designed to examine the effect of surface charge and hydrophobicity on cell adhesion, morphology, and motility. The hydrophobic nature and surface charge of different polyelectrolyte films significantly affected A7r5 cell attachment and spreading. In general, hydrophobic polyelectrolyte film surfaces, regardless of formal charge, were found to be more cytophilic than hydrophilic surfaces. On the most hydrophobic surfaces, the A7r5 cells adhered, spread, and exhibited little indication of motility, whereas on the most hydrophilic surfaces, the cells adhered poorly if at all and when present on the surface displayed characteristics of being highly motile. The two surfaces that minimized cell adhesion consisted of two varieties of a diblock copolymer containing hydrophilic poly(ethylene oxide) and a copolymer bearing a zwitterionic group AEDAPS, (3-[2-(acrylamido)-ethyldimethyl ammonio] propane sulfonate). Increasing the proportion of AEDAPS in the copolymer decreased the adhesion of cells to the surface. Cells presented with micropatterns of cytophilic and cytophobic surfaces generated by polymer-on-polymer stamping displayed a surface-dependent cytoskeletal organization and a dramatic preference for adhesion to, and spreading on, the cytophilic surface, demonstrating the utility of polyelectrolyte films in manipulating smooth muscle cell adhesion and behavior.
The kinome associated with estrogen receptor-positive status in human breast cancer (2014)

Estrogen receptor alpha (ERα) regulates and is regulated by kinases involved in several functions associated with the hallmarks of cancer. The following literature review strongly suggests that distinct kinomes exist for ERα-positive and -negative human breast cancers. Importantly, consistent with the known heterogeneity of ERα-positive cancers, different subgroups exist, which can be defined by different kinome signatures, which in turn are correlated with clinical outcome. Strong evidence supports the interplay of kinase networks, suggesting that targeting a single node may not be sufficient to inhibit the network. Therefore, identifying the important hubs/nodes associated with each clinically relevant kinome in ER+ tumors could offer the ability to implement the best therapy options at diagnosis, either endocrine therapy alone or together with other targeted therapies, for improved overall outcome.
Necrotising Enterocolitis in Very Low Birth Weight Infants in Italy: Incidence and Non‐nutritional Risk Factors (2008)

Incidence and non-nutritional risk factors were estimated for necrotising enterocolitis in 2035 very low birth weight infants, admitted to 14 tertiary-level neonatal intensive care units in Lombardy, northern Italy. There were 62 necrotising enterocolitis cases, with an overall incidence of 3.1%. After adjustment for gestational age and centre, the risk factors were mechanical ventilation, patent ductus arteriosus, and late-onset sepsis, whereas surfactant treatment was associated with decreased risk. Significant variations in necrotising enterocolitis incidence among hospitals were found.
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells (2011)

The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting cells (APCs) during antigen recognition. Recent evidence points to the transfer of RNA via exosomes as a novel mode of intercellular communication. Here we show that exosomes of T, B and dendritic immune cells contain microRNA (miRNA) repertoires that differ from those of their parent cells. We investigate whether miRNAs are exchanged during cognate immune interactions, and demonstrate the existence of antigen-driven unidirectional transfer of miRNAs from the T cell to the APC, mediated by the delivery of CD63+ exosomes on immune synapse formation. Inhibition of exosome production by targeting neutral sphingomyelinase-2 impairs transfer of miRNAs to APCs. Moreover, miRNAs transferred during immune synapsis are able to modulate gene expression in recipient cells. Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis.
The Structure of the Sugar Residue in Glycated Human Serum Albumin and Its Molecular Recognition by Phenylboronate (2003)

Quantification of the extent of glycation of human serum albumin (HSA) and of haemoglobin provides a record of average mid- and long-term blood-sugar concentrations, respectively; this is very useful for the management of diabetes. The reaction of D-glucose with propylamine affords the corresponding Schiff base, N-propylamino-D-glucoside, in the cyclic form. This compound is not stable: upon standing or treatment with acid it is converted, by an Amadori rearrangement, into N-propylfructosamine. Both amino sugars occur predominantly in the beta-pyranose form. Phenylboronate forms highly stable boronate esters through binding of the cis 1,2-diol moiety in the furanose form of N-propylfructosamine. Between pH 5 and 10, an electrostatic interaction between the protonated amino group and the negatively charged boronate moiety affords an additional stabilisation of the ester. The Schiff base, however, has no observable interaction with phenylboronate. In aqueous solution the Schiff base is in equilibrium with propylamine and glucose. Upon addition of phenylboronate, this equilibrium shifts to the side of glucose due to the formation of highly stable phenylboronate esters of the beta-furanose form of this compound. After Amadori rearrangement, the sugar moieties in glycated human serum albumin have a similar structure, they occur as an equilibrium of the beta-pyranose (59%), alpha-furanose (19%) and beta-furanose (24%) anomers. The open form was not observed. The beta-furanose anomer is selectively recognised by phenylboronate.
Zinc content of theBacillus anthracislethal factor (1994)

We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicating that all three zinc atoms are tightly bound to the protein. Lethal factor contains the highly conserved zinc-binding consensus sequence, HExxH, that is present in all known zinc metalloproteases. In addition, lethal factor contains an inverted form of this motif, HxxDH, which may also be involved in zinc binding. Using a large array of protease model substrates, however, we were unable to detect an endogenous protease activity for lethal factor.
Prevention of Spontaneous Breast Carcinoma by Prophylactic Vaccination with Dendritic/Tumor Fusion Cells (2003)

Genetically modified mice with spontaneous development of mammary carcinoma provide a powerful tool to study the efficacy of tumor vaccines, since they mimic breast cancer development in humans. We used a transgenic murine model expressing polyomavirus middle T oncogene and mucin 1 tumor-associated Ag to determine the preventive effect of a dendritic/tumor fusion cell vaccine. The MMT (a transgenic murine model) mice developed mammary carcinoma between the ages of 65-108 days with 100% penetrance. No spontaneous CTL were detected. However, prophylactic vaccination of MMT mice with dendritic/tumor fusion cells induced polyclonal CTL activity against spontaneous mammary carcinoma cells and rendered 57-61% of the mice free of the disease at the end of experiment (180 days). Furthermore, the level of CTL activity was maintained with multiple vaccinations. The antitumor immunity induced by vaccination with dendritic/tumor fusion cells reacted differently to injected tumor cells and autochthonous tumor. Whereas the injected tumor cells were rejected, the autochthonous tumor evaded the attack and was allowed to grow. Collectively these results indicate that prophylactic vaccination with dendritic/tumor fusion cells confers sufficient antitumor immunity to counter the tumorigenesis of potent oncogenic products. The findings in the present study are highly relevant to cancers in humans.
Nanophotonic Ion Production from Silicon Microcolumn Arrays (2009)

Nanoantennas for ions: Silicon microcolumn arrays harvest light from a laser pulse to produce ions. The system behaves like a quasi-periodic antenna array with ion yields that show profound dependence on the plane of polarization and the angle of incidence of the laser beam. Photonic ion sources promise to enable enhanced control of ion production on a micro- and nanometer scale and direct integration with miniaturized analytical devices.
Behavioural analysis of the efficacy of treatments for injuries to the spinal cord in animals (2004)

There have been very few clinical trials evaluating therapies for naturally occurring spinal cord injury in dogs and cats. This review describes the methods suitable for evaluating the behavioural recovery of animals with spinal cord injuries, in either a clinical or a laboratory setting. A list of commonly used methods for evaluating behavioural recovery in animals is provided, both in the clinical and laboratory setting; the tests, their limitations and benefits and specific recommendations for their use are also discussed in more depth.
<title>Adaptive array beamforming with fixed-point arithmetic matrix inversion using Givens rotations</title> (2001)

Adaptive array systems require the periodic solution of the well-known w=R<SUB>1</SUB>v equation in order to compute optimum adaptive array weights. The covariance matrix R is estimated by forming a product of noise sample matrices X:R=X<SUP>H</SUP>X. The operations-count cost of performing the required matrix inversion in real time can be prohibitively high for a high bandwidth system with a large number of sensors. Specialized hardware may be required to execute the requisite computations in real time. The choice of algorithm to perform these computations must be considered in conjunction with the hardware technology used to implement the computation engine. A systolic architecture implementation of the Givens rotation method for matrix inversion was selected to perform adaptive weight computation. The bit-level systolic approach enables a simple ASIC design and a very low power implementation. The bit-level systolic architecture must be implemented with fixed-point arithmetic to simplify the propagation of data through the computation cells. The Givens rotation approach has a highly parallel implementation and is ideally suited for a systolic implementation. Additionally, the adaptive weights are computed directly from the sample matrix X in the voltage domain, thus reducing the required dynamic range needed in carrying out the computations. An analysis was performed to determine the required fixed-point precision needed to compute the weights for an adaptive array system operating in the presence of interference. Based on the analysis results, it was determined that the precision of a floating-point computation can be well approximated with a 13-bit to 19-bit word length fixed point computation for typical system jammer-to-noise levels. This property has produced an order-of-magnitude reduction in required hardware complexity. A synthesis-based ASIC design process was used to generate preliminary layouts. These layouts were used to estimate the area and throughput of the VLSI QR decomposition architecture. The results show that this QR decomposition process, when implemented into a full-custom design, provides a computation time that is two orders of magnitude faster than a state-of-the-art microprocessor.
Acute anterior myocardial infarction due to aortosaphenous vein graft occlusion with very large thrombus burden. (2007)

A 75-year-old man, with a previous history of myocardial infarction and three-vessel coronary artery bypass grafting, presented with an acute anterior ST-elevation myocardial infarction. The vein graft to the left anterior descending artery was occluded with heavy thrombus burden, and the other grafts were patent. After administering a bolus dose of tirofiban and then undergoing percutaneous coronary intervention without stenting to the left anterior descending artery saphenous vein graft, intracoronary thrombolytic infusion was performed to maintain the patency of the vein graft. The patient was asymptomatic after medical follow-up. This may be an effective treatment option in patients with large thrombus burden and requires further investigation through large-scale trials.
Improving Word Alignment with Bridge Languages (2007)

We describe an approach to improve Statistical Machine Translation (SMT) performance using multi-lingual, parallel, sentence-aligned corpora in several bridge languages. Our approach consists of a sim-ple method for utilizing a bridge language to create a word alignment system and a proce-dure for combining word alignment systems from multiple bridge languages. The final translation is obtained by consensus de-coding that combines hypotheses obtained using all bridge language word alignments. We present experiments showing that mul-tilingual, parallel text in Spanish, French, Russian, and Chinese can be utilized in this framework to improve translation performance on an Arabic-to-English task. 1
B模式：拯救地球 延续文明 (2004)

今天,全世界可能有上千个会议在召开,但我想没有任何一次会议会比我们这个会议更重要,所以我非常荣幸能够来到这里,与各位来自中国和其它国家的著名环境专家坐在一起.同时,我也非常高兴能够见到曲格平先生,他在环境领域一直扮演着领袖的角色,而且我很高兴能够在潘岳局长演讲之后进行我的发言.
MPTP-induced parkinsonism in human and non-human primates--clinical and experimental aspects. (1984)

In this review, we describe the discovery of a new neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine or MPTP, which appears to be highly selective for the substantia nigra of human and non-human primates. This compound appears to produce a "pure" parkinsonian state more consistently than most, if not all previously described neurotoxins. Identification of its unique properties has already led to the development of an animal model for Parkinson's disease in the monkey. In this paper we review the history of the compound, and describe its clinical effects in man and monkeys. Experiments are reviewed which suggest that the "4-5" double bond in the nitrogen-containing ring is key for this compound to exert its toxic effects, probably serving as a starting point for oxidation of MPTP to its quaternary amine, 1-methyl-4-phenylpyridinium ion (MPP+). MPTP appears to be rapidly converted to the latter compound in all tissues studied to date, including brain. We believe that this newly recognized nigrotoxin holds promise as a tool for the study of Parkinson's disease and the nigrostriatal dopaminergic system of the brain.
Reactions of Microsolvated Organic Compounds at Ambient Surfaces: Droplet Velocity, Charge State, and Solvent Effects (2012)

The exposure of charged microdroplets containing organic ions to solid-phase reagents at ambient surfaces results in heterogeneous ion/surface reactions. The electrosprayed droplets were driven pneumatically in ambient air and then electrically directed onto a surface coated with reagent. Using this reactive soft landing approach, acid-catalyzed Girard condensation was achieved at an ambient surface by directing droplets containing Girard T ions onto a dry keto-steroid. The charged droplet/surface reaction was much more efficient than the corresponding bulk solution-phase reaction performed on the same scale. The increase in product yield is ascribed to solvent evaporation, which causes moderate pH values in the starting droplet to reach extreme values and increases reagent concentrations. Comparisons are made with an experiment in which the droplets were pneumatically accelerated onto the ambient surface (reactive desorption electrospray ionization, DESI). The same reaction products were observed but differences in spatial distribution were seen associated with the "splash" of the high velocity DESI droplets. In a third type of experiment, the reactions of charged droplets with vapor phase reagents were examined by allowing electrosprayed droplets containing a reagent to intercept the headspace vapor of an analyte. Deposition onto a collector surface and mass analysis showed that samples in the vapor phase were captured by the electrospray droplets, and that instantaneous derivatization of the captured sample is possible in the open air. The systems examined under this condition included the derivatization of cortisone vapor with Girard T and that of 4-phenylpyridine N-oxide and 2-phenylacetophenone vapors with ethanolamine.
G-CSF-Stimulated PMN in Immunotherapy of Breast Cancer with a Bispecific Antibody to FcγRI and to HER-2/neu (MDX-210) (1995)

Myeloid cells can mediate tumor cell cytotoxicity via certain receptors for immunoglobulins. Among the different Fc receptors, the high-affinity IgG receptor (Fc gamma RI, CD64) is a promising trigger molecule because it is selectively expressed on effector cells, including monocytes/macrophages and granulocyte colony-stimulating factor (G-CSF)-primed neutrophils. In vitro, a bispecific antibody (BsAb) (MDX-210, constructed by chemically cross-linking F(ab') fragments of monoclonal antibody (mAb) 520C9 to HER-2/neu and F(ab') fragments of mAb 22 to Fc gamma RI) mediated effective lysis of HER-2/neu overexpressing breast cancer cell lines. HER-2/neu (c-erbB2) is overexpressed in approximately 30% of breast and ovarian carcinomas and is a target for immunotherapy in clinical trials. In vitro assays showed Fc gamma RI-positive neutrophils to constitute a major effector cell population during G-CSF therapy. Based on these preclinical data and a preceding study at Dartmouth (New Hampshire) with a single dose of MDX-210 alone, a combination of G-CSF and MDX-210 is tested in a phase I study in breast cancer patients. In this study, patients receiving G-CSF are treated with escalating single doses of MDX-210. This therapy was generally well tolerated by the treated patients, some of whom reacted with fever and short periods of chills, which were temporally related to elevated plasma levels of IL-6 and TNF-alpha. After MDX-210 application, a transient decrease in the total white blood count and absolute neutrophil count (ANC) was observed. During G-CSF application, isolated neutrophils were highly cytotoxic in the presence of MDX-210 in vitro. These data indicate a potential role for G-CSF and BsAb in immunotherapy.
Core fucosylation of N-linked glycans in leukocyte adhesion deficiency/congenital disorder of glycosylation IIc fibroblasts (2005)

Leukocyte adhesion deficiency/congenital disorder of glycosylation IIc (LAD II/CDG IIc) is a genetic disease characterized by a decreased expression of fucose in glycoconjugates, resulting in leukocyte adhesion deficiency and severe morphological and neurological abnormalities. The biochemical defect is a reduced transport of guanosine diphosphate-L-fucose (GDP-L-fucose) from cytosol into the Golgi compartment, which reduces its availability as substrate for fucosyltransferases. The aim of this study was to determine the effects of a limited supply of GDP-L-fucose inside the Golgi on core fucosylation (alpha1,6-fucose linked to core N-acetylglucosamine [GlcNAc]) of N-linked glycans in LAD II fibroblasts. The results showed that, although [3H]fucose incorporation was generally reduced in LAD II cells, core fucosylation was affected to a greater extent compared with other types of fucosylation of N-linked oligosaccharides. In particular, core fucosylation was found to be nearly absent in biantennary negatively charged oligosaccharides, whereas other types of structures, in particular triantennary neutral species, were less affected by the reduction. Expression and activity of alpha1,6-fucosyltransferase (FUT8) in control and LAD II fibroblasts were comparable, thus excluding the possibility of a decreased activity of the transferase. The data obtained confirm that the concentration of GDP-L-fucose inside the Golgi can differentially affect the various types of fucosylation in vivo and also indicate that core fucosylation is not dependent only on the availability of GDP-L-fucose, but it is significantly influenced by the type of oligosaccharide structure. The relevant reduction in core fucosylation observed in some species of oligosaccharides could also provide clues for the identification of glycans involved in the severe developmental abnormalities observed in LAD II.
Designing with Care: The Future of Pervasive Healthcare (2009)

Humane care—which is an important design paradigm relevant for pervasive healthcare—means designing systems with care, keeping in mind both users and other stakeholders. Understanding human values is a key factor in creating any successful healthcare application and is essential to avoid dehumanizing and stigmatizing users. By considering human values, designers and developers can create pervasive healthcare applications that better fit users' needs and desires. The Designing with Care 09 workshop aimed to bring together researchers, designers, and practitioners to share their experiences with incorporating values into the design of pervasive healthcare systems and to help understand the sensitive issues involved in designing for healthcare. Based on the findings from the workshop, the present state of the art is discussed, and a research agenda is presented.
Inhibition of MDM2 attenuates neointimal hyperplasia via suppression of vascular proliferation and inflammation (2011)

The MDM2 antagonist nutlin-3 inhibits VSMC proliferation, migration, and NF-κB activation, and also attenuates neointimal hyperplasia after vascular injury in mice, which is associated with suppression of vascular cell proliferation and an inflammatory response. Targeting MDM2 might be a potential therapeutic strategy for the treatment of vascular proliferative diseases.
Applications of inductive logic programming (1995)

Techniques of machine learning have been successfully applied to various problems [1, 12]. Most of these applications rely on attribute-based learning, exemplified by the induction of decision trees as in the program C4.5 [20]. Broadly speaking, attribute-based learning also includes such approaches to learning as neural networks and nearest neighbor techniques. The advantages of attribute-based learning are: relative simplicity, efficiency, and existence of effective techniques for handling noisy data. However, attribute-based learning is limited to non-relational descriptions of objects in the sense that the learned descriptions do not specify relations among the objects' parts. Attribute-based learning thus has two strong limitations: the background knowledge can be expressed in rather limited form, and the lack of relations makes the concept description language inappropriate for some domains.
Simple Fluorescent Pyrimidine Analogues Detect the Presence of DNA Abasic Sites (2005)

A family of simple pyrimidine analogues has been synthesized, and their photophysical properties have been investigated. The most responsive of the family was incorporated in DNA. This isosteric fluorescent DNA analogue monitors denaturation of a DNA duplex via fluorescence and positively detects the presence of abasic sites in DNA duplexes.