gebhart/patrepeval huggingface dataset:
Overexpression of the intact thyrotropin receptor in a human thyroid carcinoma cell line (1993)

Although thyrotropin is known to regulate thyroid cell differentiation and proliferation, human thyroid carcinoma cells are relatively insensitive or resistant to TSH stimulation. The expression levels of TSH receptor are significantly lower in carcinoma tissues than in normal tissues. Furthermore, in vitro human thyroid cell growth is not regulated by TSH itself. We, therefore, isolated neomycin-resistant stable human thyroid carcinoma cell (WRO cell) transfectants overexpressing intact human TSH receptor to evaluate the functional role of TSH receptor on carcinoma cells. Southern blot analysis confirmed incorporation and amplification of human TSH receptor complementary DNA sequences into genomic DNA. Northern gel analysis and reverse transcriptase-polymerase chain reaction analysis revealed the presence of specific TSH receptor messenger RNA (4.0 kilobases), and the specific binding and the affinity of [125I]TSH on stably transfected WRO cells were demonstrated compared to wild type. Nevertheless, impaired cAMP production to transfectants by TSH was observed. cAMP production was confirmed after stimulation of both wild type and transfectants by forskolin, cholera toxin, and isoproterenol. In contrast, TSH could affect the cytoplasmic calcium mobilization immediately after the addition of TSH to WRO transfectants. These results suggest that the impairment of TSH action on human thyroid carcinoma cells is not due to a major structural abnormality of the TSH receptor, reduction in the receptor number, or receptor affinity, but much more likely due to a TSH receptor-guanyl nucleotide-binding protein coupling defect.
Terminology for high-risk and vulnerable coronary artery plaques (2004)

A group of investigators met for two days in Santorini, Greece, to discuss progress in the field of identification and treatment of high risk/vulnerable atherosclerotic plaques and patients. Many differences in the manner in which terms are being utilized were noted. It was recognized that increased understanding of the pathophysiology of coronary thrombosis and onset of acute coronary syndromes has created the need for agreement on nomenclature. The participants spent considerable time discussing the topic and reached agreement on their own usage of the terms as described below. It is the hope that this usage might be of value to the larger community of scientists working in this field, and that widespread adoption of a common nomenclature would accelerate progress in the prevention of acute coronary events.
The Clinical Usefulness of the Platelet Aggregation Test for the Diagnosis of Heparin-Induced Thrombocytopenia (1993)

The platelet aggregation test is widely used for the diagnosis of heparin-induced thrombocytopenia (HIT), a potentially serious complication of heparin therapy. We have evaluated its sensitivity and specificity in comparison with those of the 14C-serotonin release test. The sensitivity of the platelet aggregation test was found to vary with the heparin concentration and the donor of the platelets used in the test. The optimal heparin concentrations were between 0.1 and 1.0 U/ml. Using these heparin concentrations, the mean sensitivity varied from 39% (with the least reactive platelets) to 81% (with the most reactive platelets). In comparison, the sensitivity of the release test ranged from 65% to 94%. The specificities of the platelet aggregation test were 82%, 90% and 100% for the following control groups: (1) non-thrombocytopenic patients given heparin, (2) patients with thrombocytopenia due to other causes, and (3) normal controls not given heparin, respectively. The corresponding specificities for the release test was 94%, 90% and 100%. The specificities can be further increased to 100% for all controls with the adoption of a two-point system which defines a positive result as one in which platelet aggregation occurs with a low heparin concentration (0.5 U/ml) but not with 100 U heparin/ml. For optimal results, a two-point platelet aggregation test should be performed with heparin concentrations of 0.5 and 100 U/ml and using platelets of more reactive donors.
Bacterial eye infection in neonates, a prospective study in a neonatal unit. (1998)

One hundred and fifty five neonates with conjunctivitis admitted into the neonatal unit at the Lagos University Teaching Hospital were microbiologically investigated. This was to determine the bacterial aetiologic agent(s) in neonatal eye infection and highlight some risk factors. Antimicrobial susceptibility testing was done on the pathogens isolated using the diskagar diffusion method. The incidence of conjuctivitis in the newborn was 18 per 1000 live births. Predisposing factor noted were vaginal delivery, asphyxia neonatorum and prolonged rupture of membrane. Pathogens predominantly isolated were Staphylococcus aureus (37.4%), Coagilase negative Staphylococci (12.3%), Klebsiella pneumoniae (12.9%) and Pseudomonas aeruginosa (8.2%). Antimicrobial suscepibility results revealed varied degrees of susceptibility to ofloxacin (75%), Cloxacillin, erythromycin, Gentamicin and augumentin (30%) by the gram positive bacteria while most of the gram negative were susceptible to colistin and ofloxacin (above 90%). The high incidence of bacterial eye infection should be minimized by the elimination of the risk factors and adoption of stringent aseptic measures in the care of the neonate.
Delta‐Toxin fromStaphylococcus aureusas a Costimulator of Human Neutrophil Oxidative Burst (1997)

Delta-toxin from Staphylococcus aureus is responsible for various pathophysiologic effects. By studying different cell types in binding of delta-toxin in low, noncytotoxic concentrations, a specific binding of fluorescein-labeled delta-toxin to neutrophils and monocytes was found. Studying direct effects of delta-toxin on neutrophils, a dose-dependent up-regulation of complement receptor 3 expression was found. Oxygen radical production, as determined by Luminol-enhanced chemiluminescence, was not directly induced by delta-toxin, and this toxin was also unable to prime neutrophils for an enhanced response to FMLP or complement-opsonized zymosan. However, the priming response induced by lipopolysaccharide or tumor necrosis factor-alpha (TNF-alpha) was significantly further enhanced in the presence of delta-toxin. Furthermore, as a direct effect on human monocytes, delta-toxin induced TNF-alpha production. These data provide evidence that delta-toxin has direct and indirect effects on the activity of neutrophils and monocytes with regard to its proinflammatory capacity.
Update on obinutuzumab in the treatment of B-cell malignancies (2014)

Clinical data are now emerging confirming the promise of the initial preclinical data that demonstrated superior efficacy of obinutuzumab over rituximab at similar dosing. The emerging randomized Phase III data from older comorbid patients with previously untreated CLL demonstrated significant improvements in molecular remission rates and median progression-free survival of obinutuzumab plus chlorambucil versus rituximab plus chlorambucil. This emerging data provide reasons to be optimistic that outcomes for patients with B-cell malignancies can be further improved with obinutuzumab.
An optimal algorithm for detecting weak visibility of a polygon (1990)

Notation and a theorem are presented which, using a result of B. Chazelle and L.J. Guibas (1985), enable the authors to design an O(n log n) algorithm for reporting all visibility edges of a given n-vertex polygon. Improving on this bound to O(n) is presently focused upon. This problem is solved for polygons with at least one given visibility edge. It is assumed that both endpoints of this edge are convex vertices. Subsequently, it is shown how to drop this restriction. The general case of detecting weak edge visibility of an arbitrary simple polygon is dealt with.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Theory and experiments of concentration polarization and ion focusing at microchannel and nanochannel interfaces (2010)

In this tutorial review aimed at researchers using nanofluidic devices, we summarize the current state of theoretical and experimental approaches to describing concentration polarization (CP) in hybrid microfluidic-nanofluidic systems. We also analyze experimental results for these systems and place them in the context of recent theoretical developments. We then extend the theory to explain the behavior of both positively and negatively charged, low-concentration, analyte species in systems with CP. We conclude by discussing several applications of CP in microfluidics.
Specific HIV integration sites are linked to clonal expansion and persistence of infected cells (2014)

The persistence of HIV-infected cells in individuals on suppressive combination antiretroviral therapy (cART) presents a major barrier for curing HIV infections. HIV integrates its DNA into many sites in the host genome; we identified 2410 integration sites in peripheral blood lymphocytes of five infected individuals on cART. About 40% of the integrations were in clonally expanded cells. Approximately 50% of the infected cells in one patient were from a single clone, and some clones persisted for many years. There were multiple independent integrations in several genes, including MKL2 and BACH2; many of these integrations were in clonally expanded cells. Our findings show that HIV integration sites can play a critical role in expansion and persistence of HIV-infected cells.
Detecting near-duplicates for web crawling (2007)

Near-duplicate web documents are abundant. Two such documents differ from each other in a very small portion that displays advertisements, for example. Such differences are irrelevant for web search. So the quality of a web crawler increases if it can assess whether a newly crawled web page is a near-duplicate of a previously crawled web page or not. In the course of developing a near-duplicate detection system for a multi-billion page repository, we make two research contributions. First, we demonstrate that Charikar's fingerprinting technique is appropriate for this goal. Second, we present an algorithmic technique for identifying existing f-bit fingerprints that differ from a given fingerprint in at most k bit-positions, for small k. Our technique is useful for both online queries (single fingerprints) and all batch queries (multiple fingerprints). Experimental evaluation over real data confirms the practicality of our design.
A Family of Optimal Locally Recoverable Codes (2014)

A code over a finite alphabet is called locally recoverable (LRC) if every symbol in the encoding is a function of a small number (at most <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</i> ) other symbols. We present a family of LRC codes that attain the maximum possible value of the distance for a given locality parameter and code cardinality. The codewords are obtained as evaluations of specially constructed polynomials over a finite field, and reduce to a Reed-Solomon code if the locality parameter <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</i> is set to be equal to the code dimension. The size of the code alphabet for most parameters is only slightly greater than the code length. The recovery procedure is performed by polynomial interpolation over <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</i> points. We also construct codes with several disjoint recovering sets for every symbol. This construction enables the system to conduct several independent and simultaneous recovery processes of a specific symbol by accessing different parts of the codeword. This property enables high availability of frequently accessed data (“hot data”).
Asymmetric Catalysis with CO2:  Direct Synthesis of Optically Active Propylene Carbonate from Racemic Epoxides (2004)

This communication describes a convenient route to optically active propylene carbonate by a catalytic kinetic resolution process resulting from the coupling reaction of CO2 and racemic epoxides using simple chiral SalenCo(III)/quaternary ammonium halide catalyst systems.
Putative Seedling Ferulate Ester (sfe) Maize Mutant: Morphology, Biomass Yield, and Stover Cell Wall Composition and Rumen Degradability (2010)

ABSTRACT Ferulate cross‐linking of lignin to arabinoxylan contributes to poor cell wall degradability of grass forages. We hypothesized that reduced ferulate ester deposition will result in formation of fewer ferulate cross links and improved degradability. Objectives were to determine if the putative seedling ferulate ester ( sfe ) maize ( Zea mays L.) mutant, selected for reduced ferulate esters in seedling leaves, (i) exhibits reduced ferulate ethers; (ii) alters morphology, yield, and cell wall concentration and composition; and (iii) changes degradability. Four near‐isogenic sfe lines and backcrosses to W23 were compared with the inbred W23 in replicated field trials. Leaf blade, sheath, and stem of immature (1‐m plant height) and mature (silage) growth stages were analyzed for ferulate esters and ethers, cell wall concentration and composition, and in vitro rumen degradability. The sfe lines were taller and had greater internode cross‐sectional area than W23. Plant yield was greater at silage maturity for sfe lines and backcross lines yielded more than sfe Mature plant parts of sfe lines had lower ferulate ester concentrations than W23. At silage stage sfe lines had less ferulate ethers, small reductions in cell wall concentration, and shifts in composition. Immature samples were inconsistent for cell wall traits and backcross lines were generally intermediate, regardless of maturity. Cell wall degradability was higher for the sfe mutant as hypothesized. Reducing ferulate‐mediated cross‐linking of lignin to arabinoxylan in the cell walls of maize stover appears to be an effective strategy for improving cell wall degradability, and the sfe mutant may offer an opportunity to identify a gene controlling ferulate deposition in grasses.
Neuronal traits of clonal cell lines derived by fusion of dorsal root ganglia neurons with neuroblastoma cells. (1985)

In an attempt to immortalize the gene products of single neurons, somatic cell hybrids were produced by fusion of embryonic rat dorsal root ganglion (DRG) neurons with mouse neuroblastoma cells. Embryonic day 13 rat DRGs were fused with mouse neuroblastoma cells deficient in hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8). The hybrid cells were selected in medium with 100 microM hypoxanthine/1 microM aminopterin/12 microM thymidine to eliminate the neuroblastoma cells and with cis-hydroxyproline to retard fibroblast growth. Of the 17 lines derived, 4 manifested neuronal properties and were cloned. These lines retain both rat and mouse chromosomes and synthesize characteristic rat and mouse isoenzymes. Neuronal gangliosides, action potentials, and extensive neurite-like processes are exhibited by these hybrid cells, properties characteristic of DRG neurons but not of the neuroblastoma parent. Each line manifests a unique combination of action-potential properties and cell-surface markers, suggesting the selective expression of subsets of DRG neuronal genes. All of these neuronal properties are expressed constitutively, without the need for chemical induction or mitotic inhibition, and stably, without diminution after at least 5 months in culture. These lines may prove useful in the identification and isolation of gene products that characterize individual or small subsets of DRG neurons.
Isolation and characterization of a 60-residue intestinal peptide structurally related to the pancreatic secretory type of trypsin inhibitor: influence on insulin secretion. (1989)

We have isolated from pig intestine a 60-residue polypeptide initially identified by its inhibition of glucose-induced insulin secretion from perfused pancreas. The amino acid sequence of this porcine polypeptide was determined and found to be markedly similar to that of the pancreatic secretory trypsin inhibitor (41% residue identities). Furthermore, the disulfide arrangements of these two proteins appear identical, suggesting related overall conformations. However, the polypeptide, now named PEC-60 (peptide with N-terminal glutamic acid, C-terminal cysteine, and a total of 60 residues), was found not to inhibit trypsin. The amino acid sequence is also similar to that of a peptide recently isolated from rat bile/pancreatic juice which stimulates the release of cholecystokinin. The biological role of PEC-60 is not known, but the effect on insulin secretion and the homologies observed suggest important biological activities and interesting structural relationships.
Using pattern recognition in electron density map interpretation (2000)

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Diffusion coefficient measurement using a temperature‐controlled fluid for quality control in multicenter studies (2011)

The ice-water phantom described here is a practical and universal approach to validate the accuracy of ADC measurements with ever changing MRI sequence and hardware design and can be readily implemented in multicenter clinical trial designs.
Stem cell concepts renew cancer research (2008)

Although uncontrolled proliferation is a distinguishing property of a tumor as a whole, the individual cells that make up the tumor exhibit considerable variation in many properties, including morphology, proliferation kinetics, and the ability to initiate tumor growth in transplant assays. Understanding the molecular and cellular basis of this heterogeneity has important implications in the design of therapeutic strategies. The mechanistic basis of tumor heterogeneity has been uncertain; however, there is now strong evidence that cancer is a cellular hierarchy with cancer stem cells at the apex. This review provides a historical overview of the influence of hematology on the development of stem cell concepts and their linkage to cancer.
Synthesis of a Sulfahydantoin Library (2001)

A five-step solid-phase synthesis of sulfahydantoins from alpha-amino acids and aldehydes was developed. The synthetic method allows the use of hindered amino acids, including Val, Phg, and Aib, and use of aromatic aldehydes substituted with electron-withdrawing and -donating groups. Some limitations were encountered with amino acids with reactive side chains. A small but diverse library of compounds was produced for biological testing.
Optical Coherence Tomography of Ocular Diseases (2024)

This work shows the retina in normal and diseased states using the innovative OCT 3000. Topics include macular and other retinal diseases, glaucoma, and a description of OCT technology. An appendix is included for those interested in learning more about the principles of operation of this medical diagnostic imaging technology. The text's primary objective is to illustrate the appearance of the eye in health and disease, comparing conventional clinical technologies with OCT. This method introduces the clinician to the manifestations of disease as elucidated by OCT, while presenting the more familiar funduscopic and fluorescein angiographic appearance side-by-side. The text should provide a clinical reference for the retinal and glaucoma specialist which shows how to utilize and interpret OCT imaging to enhance diagnostic sensitivity and specificity as well as to enhance therapeutic decision-making and monitor the outcome of treatment.
The ZIC gene family in development and disease (2005)

The human ZIC gene family is comprised of five members encoding zinc-finger transcription factors, which are the vertebrate homologs of the Drosophila odd-paired gene. Mutations in ZIC genes in humans have recently been implicated in a wide variety of congenital malformations, including Dandy-Walker malformation, holoprosencephaly, neural tube defects, and heterotaxy. Mutant analysis of these genes in mice has underscored the conserved developmental roles of these genes. Further, this analysis has begun to elucidate the molecular and developmental mechanisms underlying these important birth defects.
Green‐fluorescent protein as a new vital marker in plant cells (1995)

The green-fluorescent protein (GFP) from jellyfish Aequorea victoria has been used as a convenient new vital marker in various heterologous systems. However, it has been problematic to express GFP in higher eukaryotes, especially in higher plants. This paper reports that either a strong constitutive or a heat-shock promoter can direct the expression of GFP which is easily detectable in maize mesophyll protoplasts. In this single-cell system, bright green fluorescence emitted from GFP is visible when excited with UV or blue light even in the presence of blue fluorescence from the vacuole or the red chlorophyll autofluorescence from chloroplasts using a fluorescence microscope. No exogenous substrate, co-factor, or other gene product is required. GFP is very stable in plant cells and shows little photobleaching. Viable cells can be obtained after fluorescence-activated cell sorting based on GFP. The paper further reports that GFP can be detected in intact tissues after delivering the constructs into Arabidopsis leaf and root by microprojectile bombardment. The successful detection of GFP in plant cells relies on the use of a universal transcription enhancer from maize or the translation enhancer from tobacco mosaic virus (TMV) to boost the expression. This new reporter could be used to monitor gene expression, signal transduction, co-transfection, transformation, protein trafficking and localization, protein-protein interaction, cell separation and purification, and cell lineage in higher plants.
A novel goal oriented concept for situation representation for ADAS and automated driving (2014)

Being aware of the environment is crucial for the task of driving a vehicle. With increasing degree of automation, the complexity of the relevant environment which has to be represented internally is growing. For humans this internal representation is called situation awareness. In the intelligent transportation community it is often called situation representation although there is no broadly accepted definition of the term situation. In this work we discuss existing concepts and employ the concept of situation awareness to generalize and extend the existing concepts to a goal oriented concept for situation representation. The concept can be exploited for the deduction of requirements for the perception process from the (driving) goals of a system on different levels of abstraction.