gebhart/patrepeval huggingface dataset:
Antisense Oligodeoxynucleotides Directed Against Kv1.5 mRNA Specifically Inhibit Ultrarapid Delayed Rectifier K+Current in Cultured Adult Human Atrial Myocytes (1997)

Several cloned K+ channel subunits are candidates to underlie macroscopic currents in the human heart, but direct evidence bearing on their role is lacking. The Kv1.5 K+ channel subunit has been suggested to play a potential role in human cardiac ultrarapid delayed rectifier (IKur) and transient outward (Ito) currents. To evaluate the role of proteins encoded by the Kv1.5 gene, we incubated cultured human atrial myocytes for 48 hours in medium containing antisense phosphorothioate oligodeoxynucleotides directed against octodecameric segments of the Kv1.5 mRNA coding sequence, the same concentration of homologous oligodeoxynucleotides with four mismatch mutations, or vehicle (control group). Cells exposed to antisense showed a highly significant (approximately 50%) reduction in IKur whether measured by step current at the end of a 400-millisecond depolarizing pulse, tail current at -20 mV, or current sensitive to a concentration of 4-aminopyridine (50 mumol/L) that is highly selective for IKur compared with control cells or cells exposed to mismatch oligodeoxynucleotides. In contrast, Ito was not different among the three experimental groups. When cultured human ventricular myocytes were exposed to Kv1.5 antisense oligodeoxynucleotides with the same controls, no changes occurred in either Ito or the sustained current at the end of a depolarizing pulse. We conclude that Kv1.5 channel subunits are essential to the expression of IKur and do not play a role in Ito in cultured human atrial myocytes. These studies provide the first direct evidence with an antisense approach for the equivalence between a macroscopic cardiac K+ current and a cloned K+ channel subunit and offer insights into the molecular electrophysiology of the human heart.
Guidelines for the Management of Dyspepsia (2005)

Dyspepsia is a chronic or recurrent pain or discomfort centered in the upper abdomen; patients with predominant or frequent (more than once a week) heartburn or acid regurgitation, should be considered to have gastroesophageal reflux disease (GERD) until proven otherwise. Dyspeptic patients over 55 yr of age, or those with alarm features should undergo prompt esophagogastroduodenoscopy (EGD). In all other patients, there are two approximately equivalent options: (i) test and treat for Helicobacter pylori (H. pylori) using a validated noninvasive test and a trial of acid suppression if eradication is successful but symptoms do not resolve or (ii) an empiric trial of acid suppression with a proton pump inhibitor (PPI) for 4-8 wk. The test-and-treat option is preferable in populations with a moderate to high prevalence of H. pylori infection (> or =10%); empirical PPI is an initial option in low prevalence situations. If initial acid suppression fails after 2-4 wk, it is reasonable to consider changing drug class or dosing. If the patient fails to respond or relapses rapidly on stopping antisecretory therapy, then the test-and-treat strategy is best applied before consideration of referral for EGD. Prokinetics are not currently recommended as first-line therapy for uninvestigated dyspepsia. EGD is not mandatory in those who remain symptomatic as the yield is low; the decision to endoscope or not must be based on clinical judgement. In patients who do respond to initial therapy, stop treatment after 4-8 wk; if symptoms recur, another course of the same treatment is justified. The management of functional dyspepsia is challenging when initial antisecretory therapy and H. pylori eradication fails. There are very limited data to support the use of low-dose tricyclic antidepressants or psychological treatments in functional dyspepsia.
Identification of collagen IV derived danger/alarm signals in insect immunity by nanoLC-FTICR MS (2009)

The immune system can be stimulated by microbial molecules as well as by endogenously derived danger/alarm signals of host origin. Using the lepidopteran model insect Galleria mellonella, we recently discovered that fragments of collagen IV, resulting from hydrolysis by microbial metalloproteinases, represent danger/alarm signals in insects. Here, we characterized immune-stimulatory peptides generated by thermolysin-mediated degradation of collagen IV using nanospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) after separation by nanoscale liquid chromatography (nanoLC). The combination of FTICR MS analysis and de novo peptide sequencing resulted in the identification of 38 specific collagen IV fragments of which several peptides included the integrin-binding motif RGD/E known from numerous mammalian immune-related proteins. Custom-synthesized peptides corresponding either to the presently identified collagen peptide GIRGEHyp or to a well-known integrin-binding RGD peptide (GRGDS) were injected into G. mellonella to determine their immune-stimulatory activities in vivo. Both peptides stimulated immune cells and systemically the expression of lysozyme and a specific inhibitor of microbial metalloproteinases. Further examination using specific MAP kinase inhibitors indicated that MEK/ERK and p38 are involved in RGD/E-mediated immune-signaling pathways, whereas JNK seems to play only a minor role.
Genotyping by apyrase-mediated allele-specific extension (2001)

This report describes a single-step extension approach suitable for high-throughput single-nucleotide polymorphism typing applications. The method relies on extension of paired allele-specific primers and we demonstrate that the reaction kinetics were slower for mismatched configurations compared with matched configurations. In our approach we employ apyrase, a nucleotide degrading enzyme, to allow accurate discrimination between matched and mismatched primer-template configurations. This apyrase-mediated allele-specific extension (AMASE) protocol allows incorporation of nucleotides when the reaction kinetics are fast (matched 3'-end primer) but degrades the nucleotides before extension when the reaction kinetics are slow (mismatched 3'-end primer). Thus, AMASE circumvents the major limitation of previous allele-specific extension assays in which slow reaction kinetics will still give rise to extension products from mismatched 3'-end primers, hindering proper discrimination. It thus represents a significant improvement of the allele-extension method. AMASE was evaluated by a bioluminometric assay in which successful incorporation of unmodified nucleotides is monitored in real-time using an enzymatic cascade.
InGaN based micro light emitting diodes featuring a buried GaN tunnel junction (2015)

GaN tunnel junctions (TJs) are grown by ammonia molecular beam epitaxy. High doping levels are achieved with a net acceptor concentration close to ∼1020 cm−3, thanks to the low growth temperature. This allows for the realization of p-n junctions with ultrathin depletion width enabling efficient interband tunneling. n-p-n structures featuring such a TJ exhibit low leakage current densities, e.g., &amp;lt;5 × 10−5 A cm−2 at reverse bias of 10 V. Under forward bias, the voltage is 3.3 V and 4.8 V for current densities of 20 A cm−2 and 2000 A cm−2, respectively. The specific series resistance of the whole device is 3.7 × 10−4 Ω cm2. Then micro-light emitting diodes (μ-LEDs) featuring buried TJs are fabricated. Excellent current confinement is demonstrated together with homogeneous electrical injection, as seen on electroluminescence mapping. Finally, the I-V characteristics of μ-LEDs with various diameters point out the role of the access resistance at the current aperture edge.
Zinc Finger Phage: Affinity Selection of Fingers with New DNA-Binding Specificities (1994)

A phage display system was developed and used to select zinc finger proteins with altered DNA-binding specificities. The three zinc fingers of the Zif268 protein were expressed on the surface of filamentous phage, and a library of variants was prepared by randomizing critical amino acids in the first zinc finger. Affinity selections, using DNA sites with base changes in the region recognized by the first finger, yielded Zif268 variants that bound tightly and specifically to the new sites. This phage system provides a tool for the study of protein-DNA interactions and may offer a general method for selecting zinc finger proteins that recognize desired target sites on double-stranded DNA.
Automated X-ray inspection of aluminum castings (1988)

The experience gained with several approaches to automatic flaw detection in X-ray images of cast aluminum wheels is described. Basic problems are mentioned, and the applicability of segmentation methods to actual inspection tasks is demonstrated. The discussion focuses on the definition, extraction, and combination of local features for pixel classification. Results of pilot tests are described briefly. Further investigations are suggested, aiming at more generality of the methods and greater stability of the segmentation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Root-Specific Expression of OsNAC10 Improves Drought Tolerance and Grain Yield in Rice under Field Drought Conditions (2010)

Drought poses a serious threat to the sustainability of rice (Oryza sativa) yields in rain-fed agriculture. Here, we report the results of a functional genomics approach that identified a rice NAC (an acronym for NAM [No Apical Meristem], ATAF1-2, and CUC2 [Cup-Shaped Cotyledon]) domain gene, OsNAC10, which improved performance of transgenic rice plants under field drought conditions. Of the 140 OsNAC genes predicted in rice, 18 were identified to be induced by stress conditions. Phylogenic analysis of the 18 OsNAC genes revealed the presence of three subgroups with distinct signature motifs. A group of OsNAC genes were prescreened for enhanced stress tolerance when overexpressed in rice. OsNAC10, one of the effective members selected from prescreening, is expressed predominantly in roots and panicles and induced by drought, high salinity, and abscisic acid. Overexpression of OsNAC10 in rice under the control of the constitutive promoter GOS2 and the root-specific promoter RCc3 increased the plant tolerance to drought, high salinity, and low temperature at the vegetative stage. More importantly, the RCc3:OsNAC10 plants showed significantly enhanced drought tolerance at the reproductive stage, increasing grain yield by 25% to 42% and by 5% to 14% over controls in the field under drought and normal conditions, respectively. Grain yield of GOS2:OsNAC10 plants in the field, in contrast, remained similar to that of controls under both normal and drought conditions. These differences in performance under field drought conditions reflect the differences in expression of OsNAC10-dependent target genes in roots as well as in leaves of the two transgenic plants, as revealed by microarray analyses. Root diameter of the RCc3:OsNAC10 plants was thicker by 1.25-fold than that of the GOS2:OsNAC10 and nontransgenic plants due to the enlarged stele, cortex, and epidermis. Overall, our results demonstrated that root-specific overexpression of OsNAC10 enlarges roots, enhancing drought tolerance of transgenic plants, which increases grain yield significantly under field drought conditions.
Enhanced depth resolution in terahertz imaging using phase-shift interferometry (2001)

We describe an imaging technique for few-cycle optical pulses. An object to be imaged is placed at the focus of a lens in one arm of a Michaelson interferometer. This introduces a phase shift of approximately π between the two arms of the interferometer, via the Gouy phase shift. The resulting destructive interference provides a nearly background-free measurement, and a dramatic enhancement in depth resolution. We demonstrate this using single-cycle pulses of terahertz radiation, and show that it is possible to resolve features thinner than 2% of the coherence length of the radiation. This technique could have important applications in low-coherence optical tomographic measurements.
Effect of a retinoid X receptor partial agonist on airway inflammation and hyperresponsiveness in a murine model of asthma (2017)

These data suggest that RXRs may be of crucial importance in the mechanism of allergic asthma and that the novel RXR partial agonist NEt-4IB may be a promising candidate for the treatment of allergic airway inflammation and airway hyperresponsiveness in a model of allergic asthma.
Environment Conditions and Biological Innovations in the European Agrarian Growth, 1819-1939 (2005)

The circumstances that were the driving forces behind Europe's economic growth beginning in the 19th century are diverse, and not easily prioritized. Until the 1970's, specifically, in Economy and Economic History, attention was focused on different institutional and technological variables, and various regularities were proposed. Nevertheless, new studies also underlined that the evolution of economic activity could not be understood considering only the new production possibilities offered by market economies. As a result, today it is also accepted that those processes can not be explained without considering two additional circumstances: the energy flows that sustained them, and the changes undergone in their transformation In this context, a question arises that takes on special importance. Which was the influence of the biological change in the economic growth?. A part of the flows of energy must be made into food, and this transformation can only happen with the participation.
EP 80317, a ligand of the CD36 scavenger receptor, protects apolipoprotein E‐deficient mice from developing atherosclerotic lesions (2005)

CD36, a type B scavenger receptor expressed on macrophages, appears to play a major role in fatty streak formation through scavenging oxidatively modified lipoproteins in the arterial wall. We tested the hypothesis that EP 80317, a novel CD36 ligand derived from the growth hormone (GH)-releasing peptide family but devoided of any GH releasing activity, exerts anti-atherosclerotic effects in apolipoprotein E-deficient (apoE-/-) mice fed an atherogenic diet from 6 wk of age. Daily subcutaneous injections of EP 80317 (300 microg/kg) or vehicle were initiated at 6, 10, 12, or 14 wk until death at 18 wk. En face analyses of the entire aortic tree revealed a striking reduction (up to 51%) of lesion areas in EP 80317-treated apoE-/- mice compared with controls. Chronic treatment with EP 80317 (12 wk) is also associated with a 30% decrease in total plasma cholesterol, suggesting potential effects of this drug on cholesterol metabolism at the intestine/hepatic levels. EP 80317 exerts both preventive and curative effects on atherosclerotic lesion progression that were shown to be reversible after cessation of treatment. At the macrophage level, EP 80317 reduced oxidized low density lipoproteins internalization and up-regulated genes involved in cholesterol efflux, including peroxisome proliferator-activated receptor gamma (PPARgamma), liver x receptor alpha (LXRalpha), and the ATP binding cassette (ABC) transporters ABCA1 and ABCG1, supporting a role in regulating peripheral cholesterol trafficking. Importantly, the effects of EP 80317 were shown to be CD36 dependent, inasmuch as no anti-atherosclerotic or hypocholesterolemic effects were observed in apoE/CD36 double-deficient mice. In addition, long-term treatment of apoE/CD36 double-deficient mice with EP 80317 did not modulate the expression of genes of the PPARgamma-LXRalpha-ABC transporters pathway. Our results suggest that EP 80317, as a CD36 ligand, might be a prototype for a novel class of anti-atherosclerotic agents.
A Locus Influencing Total Serum Cholesterol on Chromosome 19p (2000)

A genome-wide linkage study was analyzed to identify loci that influence serum lipid concentrations in Pima Indians. Linkage analyses were conducted for total cholesterol measured in 998 siblings from 292 nuclear families, for total triglycerides in 547 siblings from 188 families, and for high density lipoprotein (HDL) cholesterol in 590 siblings from 201 families. Genotypes were generated for 516 autosomal microsatellite markers. Multipoint variance components methods were used to assess linkage. The strongest evidence for linkage with total cholesterol was on chromosome 19p (lod score 3.89), in the vicinity of the marker D19S1034, which is near the low density lipoprotein receptor gene. The strongest evidence for linkage with HDL cholesterol was on chromosome 3q (lod score 2.64) near D3S3053. For triglycerides, the strongest evidence for linkage was on chromosome 2p near D2S1788 (lod score 1.70) and on chromosome 3p near D3S2406 (lod score 1.77). This genomic scan provides evidence for a locus influencing total cholesterol concentration on chromosome 19p. It also suggests a locus influencing HDL cholesterol on chromosome 3q.
Nonvolatile multilevel memories for digital applications (1998)

When thinking of semiconductor memories, it comes naturally to associate stored bits and memory cells with a one-to-one relationship. That, however, is not really a must nor necessarily the most convenient solution for data storage, since using analog signals and digital-to-analog (D/A) as well as analog-to digital (A/D) conversions a large number of bits could be memorized in a single cell, although, of course, the use of analog signals presents all drawbacks of signal-to-noise ratio that are so well known in electronics. In fact, the real question in this sense concerns the number of bits used for the A/D and D/A conversions, since the conventional (fully) digital case can be seen as the simplest realization of a general approach tending to infinitely precise analog storage (i.e. an infinite number of stored bits per cell) at the other extreme. Naturally, in the real world the conflicting aspects of density (measured in bits per cell) and noise immunity (in a general sense) should be traded off one against the other looking for optimum use of silicon area, of course depending on technology, architectures, circuits and reliability. From this point of view it is obvious that the fully digital approach based on the one-bit one-cell concept does not represent necessarily the best solution. Recently, this general question has assumed real and practical significance for nonvolatile memories, since devices storing two bits per cell are now being introduced on the market. At the same time, in a number of research labs a significant effort is currently being dedicated to the study of the limits and practical convenience of storage density considering the current state of the art in technology and circuit designs. This problem, however, presents a number of interacting aspects concerning cell concept, programming and reading schemes, and architectures and reliability that are of interest well beyond the field of nonvolatile memories, because they are ultimately dealing with the basic question of analog versus digital signals. In this contrast, the present paper considers the question of multilevel nonvolatile memories in all its interacting aspects, analyzing both the current state of the art and the future possibilities.
A comparative study of the use of executive information systems between Korea and the United States (1998)

Since their conception, EIS have been developed and adopted in many firms. The importance of EIS is now increasing with the need of accessing data from many sources under the high-velocity environment. Although many of the Korean firms are now in the process of introducing EIS with the faster growing economy, most research has been conducted on the use of EIS in Western firms. This study aims to explore the current state of EIS usage in Korean firms and make a comparison with that of the United States. Based upon Watson et al. (1992), a questionnaire instrument was constructed. The questionnaires were distributed to 136 Korean companies that were reported to use EIS and 50 of the questionnaires returned. We found that there were many differences in the use of EIS between Korea and the United States. This was mainly due to the fact that the use of EIS in Korea was at a very early stage and executives in Korea did not realize and/or understand the benefits from the use of EIS yet.
Extreme Stabilization of a Thermolysin-like Protease by an Engineered Disulfide Bond (1997)

The thermal inactivation of broad specificity proteases such as thermolysin and subtilisin is initiated by partial unfolding processes that render the enzyme susceptible to autolysis. Previous studies have revealed that a surface-located region in the N-terminal domain of the thermolysin-like protease produced by Bacillus stearothermophilus is crucial for thermal stability. In this region a disulfide bridge between residues 8 and 60 was designed by molecular modelling, and the corresponding single and double cysteine mutants were constructed. The disulfide bridge was spontaneously formed in vivo and resulted in a drastic stabilization of the enzyme. This stabilization presents one of the very few examples of successful stabilization of a broad specificity protease by a designed disulfide bond. We propose that the success of the present stabilization strategy is the result of the localization and mutation of an area of the molecule involved in the partial unfolding processes that determine thermal stability.
Panasync (2000)

File copying is frequently used to implement ad hoc management of file replicas, backups and versions. Such tasks can be assisted by appropriate applications, at the expense of introducing some restrictions to the usage patterns. In particular, this is the case of interactions involving disconnected machines and transportable media. PANASYNC tries to support these actions by introducing a set of commands for file copying and re-integration that complement the file-system commands and provide support for dependency analysis among time-stamp assisted files.
The Israelite Origins of the Mandaean People (2007)

This is considered a fact among
Los enfermeros, potenciales víctimas psicológicas en la atención de catástrofes (2003)

En situacion de catastrofe se precisa de la intervencion de muchos profesionales con campos de actuacion diferentes y con formacion especifica. Pero todos ellos, por el hecho de ser intervinientes, incluidos los profesionales sanitarios en general y los enfermeros en particular, deben ser considerados como potenciales victimas psicologicas de dicha situacion. 

Es necesario que los profesionales enfermeros esten informados y entrenados para poner en marcha los mecanismos que pueden ayudar a disminuir las consecuencias psicologicas de esa actividad. Es preciso que se considere despues de la actuacion en una catastrofe si los enfermeros necesitan una intervencion a nivel psicoemocional, que libere las cargas emocionales o bien si precisan algun otro tipo de terapia psicologica que permita reducir las secuelas de un posible estres postraumatico, del que no estan exentos de padecer a pesar de la profesionalidad y la experiencia.
Salvage Radiotherapy for Recurrent Prostate Cancer After Radical Prostatectomy (2004)

Gleason score, preradiotherapy PSA level, surgical margins, PSADT, and seminal vesicle invasion are prognostic variables for a durable response to salvage radiotherapy. Selected patients with high-grade disease and/or a rapid PSADT who were previously thought to be destined to develop progressive metastatic disease may achieve a durable response to salvage radiotherapy.