gebhart/patrepeval huggingface dataset:
Why is diabetes mellitus a stronger risk factor for fatal ischemic heart disease in women than in men? The Rancho Bernardo Study (1991)

We report here the 14-year sex-specific effect of non—insulin-dependent diabetes mellitus on the risk of fatal ischemic heart disease in a geographically defined population of men and women aged 40 through 79 years. There were 207 men and 127 women who had diabetes at baseline based on medical history or fasting hyperglycemia. They were compared with 2137 adults who had fasting euglycemia and a negative personal and family history of diabetes. The relative hazard of ischemic heart disease death in diabetics vs nondiabetics was 1.8 in men and 3.3 in women, after adjusting for age, and 1.9 and 3.3, respectively, after adjusting for age, systolic blood pressure, cholesterol, body mass index, and cigarette smoking using the Cox regression model. The sex difference in the independent contribution of diabetes to fatal heart disease was largely explained by the persistently more favorable survival rate of women (than men) without diabetes. (<i>JAMA</i>. 1991;265:627-631)
MALE STERILITY IN SOYBEAN‐AN OVERVIEW (1988)

The most common type of reproductive mutations observed in the soybean [ Glycine max (L.) Merr.] are those that induce male sterility. The high frequency of occurrence of male‐sterile mutations indicates that a number of genes influence the processes of microgametogenesis and microsporogenesis. The current knowledge of these mutations is summarized. The origins of male‐sterile mutations, their inheritance patterns, and known linkage relationships are detailed. The phenotypic expression of male‐sterile mutations, including their effects on both male and female reproduction, is discussed. The influence of environment on the expressivity of male‐sterile mutations, and the effects of male‐sterile mutations on physiological processes (senescence and nitrogen fixation) are summarized. Male‐sterile mutations have been useful in the study of soybean reproduction, genetic and cytogenetic investigations, and in evaluating the potential for commercial production of hybrid soybeans. These various applications of male‐sterile mutations are presented.
Reprogramming human somatic cells to pluripotency using RNA (2012)

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2012.
Cutting Edge: CD40 Ligand Is a Limiting Factor in the Humoral Response to T Cell-Dependent Antigens (1999)

CD40 ligand (CD40L) plays a crucial role in T cell-dependent B cell responses, but whether its abundance is a limiting factor in their development is unclear. This question was addressed in transgenic mice expressing the murine CD40L gene under the control of the IL-2-promoter (CD40Ltg+). The fraction of activated T cells from the CD40Ltg+ mice with detectable levels of surface CD40L was modestly greater (1.1- to 2-fold) than littermate controls and paralleled an approximately 1.8-fold increase in CD40L mRNA abundance. In response to trinitrophenol (TNP)-keyhole limpet hemocyanin and tetanus/diphtheria vaccine, CD40Ltg+ mice developed higher titers of high-affinity IgG and IgG1 Ab than wild-type mice. In contrast, the Ab response of CD40Ltg+ and control mice was similar in response to the T-independent Ag TNP-Ficoll. These results suggest that a modest increment in expression of CD40L accelerates the development of T-dependent responses, and that CD40L plays a limiting role in the induction of high-affinity Ab and Ab-class switching.
Broad-band thin-film signal sampler (1972)

Previous developments in high frequency signal sampling have been extended by applying thin-film and solid state techniques to the sampler design. The basic elements of a sampler are a signal-carrying transmission line, a sampling gate, a drive line for the gate pulse, and a video output. In the design reported here, a thin quartz substrate supports the circuits. To reduce signal-line reflections the gate is made the center element of a low-pass signal-line filter. The gate pulse is developed across a length of slot transmission line in the signal ground plane parallel to and beneath the signal transmission line. A beam-lead Schottky diode pair is mounted across the slot with its center node connected to the signal transmission line through the substrate.
Characterization of Mitotic Neurons Derived From Adult Rat Hypothalamus and Brain Stem (2002)

Embryonic or neonatal rat neurons retain plasticity and are readily grown in tissue culture, but neurons of the adult brain were thought to be terminally differentiated and therefore difficult to culture. Recent studies, however, suggest that it may be possible to culture differentiated neurons from the hippocampus of adult rats. We modified these procedures to grow differentiated neurons from adult rat hypothalamus and brain stem. At day 7 in tissue culture and beyond, the predominant cell types in hypothalamic and brain stem cultures had a stellate morphology and could be subdivided into two distinct groups, one of which stained with antibodies to the immature neuron marker alpha-internexin, while the other stained with the astrocyte marker GFAP. The alpha-internexin positive cells were mitotic and grew to form a characteristic two-dimensional cellular network. These alpha-internexin positive cells coimmunostained for the neuronal markers MAP2, type III beta-tubulin, and tau, and also bound tetanus toxin, but were negative for the oligodendrocyte marker GalC and also for the neurofilament triplet proteins NF-L, NF-M, and NF-H, markers of more mature neurons. Patch-clamp analysis of these alpha-internexin positive cells revealed small Ca(2+) currents with a peak current of -0.5 +/- 0.1 pA/pF at a membrane potential of -20 mV (n = 5) and half-maximal activation at -30 mV (n = 5). Na(+) currents with a peak current density of -154.5 +/- 49.8 pA/pF at a membrane potential of -15 mV (n = 5) were also present. We also show that these cells can be frozen and regrown in tissue culture and that they can be efficiently infected by viral vectors. These cells therefore have the immunological and electrophysiological properties of immature mitotic neurons and should be useful in a variety of future studies of neuronal differentiation and function.
Antioxidant imbalance in the lungs of cystic fibrosis transmembrane conductance regulator protein mutant mice (2001)

Recent studies suggest that the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) protein modulates epithelial reduced glutathione (GSH) transport and when defective creates an antioxidant imbalance. To test whether the CFTR protein modulates lung antioxidant defenses in vivo, epithelial lining fluid (ELF) and lung tissue from CFTR knockout (CFTR-KO) and wild-type (WT) mice were compared for GSH content and the activities of glutathione reductase, glutathione peroxidase, and gamma-glutamyltransferase. In the CFTR-KO mice, the ELF concentration of GSH was decreased (51%) compared with that in WT mice. The concentration of GSH in the lung tissue of CFTR-KO mice, however, was not significantly different from that in WT mice. The activities of glutathione reductase and glutathione peroxidase in the lung tissue of CFTR-KO mice were significantly increased compared with those in WT mice (48 and 28%, respectively). Tissue lipid and DNA oxidation were evaluated by measurement of thiobarbituric acid-reactive substances and 8-hydroxy-2'-deoxyguanosine, respectively. The levels of thiobarbituric acid-reactive substances and 8-hydroxy-2'-deoxyguanosine in the lung tissue of CFTR-KO mice were significantly increased compared with those in WT mice. These data support our hypothesis that a mutation in the CFTR gene can affect the antioxidant defenses in the lung and may contribute to the exaggerated inflammatory response observed in CF.
Adverse Effects Associated With High-Dose Recombinant Human Bone Morphogenetic Protein-2 Use in Anterior Cervical Spine Fusion (2006)

A significant rate of complications resulted after the use of a high dose of INFUSE in anterior cervical fusions. We hypothesize that in the cervical area, the putative inflammatory effect that contributes to the effectiveness of INFUSE in inducing fusion may spread to adjacent critical structures and lead to increased postoperative morbidity. A thorough investigation is warranted to determine the optimal dose of rhBMP-2 that will promote cervical fusion and minimize complications.
Comparison of cefotaxime, imipenem-cilastatin, ampicillin-gentamicin, and ampicillin-chloramphenicol in the treatment of experimental Escherichia coli bacteremia and meningitis (1985)

In a search for more effective antimicrobial therapy of neonatal Escherichia coli infection, newer beta-lactam antibiotics, cefotaxime and imipenem, were evaluated for their activities against a K1 E. coli strain in vitro and in vivo, and the results were compared with those of conventional therapeutic regimens for neonatal E. coli infection: ampicillin-gentamicin and ampicillin-chloramphenicol. Measured by MICs and MBCs, cefotaxime and imipenem were 8- to 512-fold more active in vitro than the older agents. For in vivo studies, the following daily doses were used: 50 mg/kg for each of imipenem and cilastatin; 100 mg/kg for each of cefotaxime, ampicillin, and chloramphenicol; and 10 mg/kg for gentamicin. At these doses, the mean bactericidal titers in blood and cerebrospinal fluid were significantly greater with newer agents than with ampicillin-gentamicin and ampicillin-chloramphenicol. However, at the doses used, the newer agents were not more effective in vivo than the older agents. This was shown by the similarities in clearance of bacteria from blood and cerebrospinal fluid, incidences of meningitis in bacteremic animals, and mortality rates. Thus, although these two newer antibiotics are more active in vitro and produce greater bactericidal titers in vivo, they do not appear to be superior to conventional regimens for treatment of neonatal E. coli bacteremia and meningitis.
A Monoclonal Antibody against Very Late Activation Antigen-4 Inhibits Eosinophil Accumulation and Late Asthmatic Response in a Guinea Pig Model of Asthma (1997)

Preferential eosinophil accumulation is a characteristic of airway inflammation in asthma. Although little is known about its mechanism, recent in vitro observations suggest that the very late activation antigen-4 (VLA-4, CD49d/CD29) and vascular cell adhesion molecule-1 (VCAM-1) adhesion pathway may be involved in specific eosinophil migration. To test this hypothesis, we studied the effect of an anti-VLA-4 monoclonal antibody (mAb) on the airway eosinophilia in a guinea pig model of asthma. Guinea pigs were sensitized by repeated inhalation of ovalbumin. After a single inhalation challenge, the animals showed a striking airway eosinophilia and late asthmatic response (LAR). In contrast, when guinea pigs were pretreated intravenously at 2 h before antigen challenge with a rat antimouse VLA-4 mAb, PS2/3, cross-reacting with guinea pig eosinophils and lymphocytes, eosinophil, basophil and lymphocyte infiltration in the tracheal wall was significantly inhibited as well as LAR in a dose-dependent manner. These results suggest that VLA-4 plays a critical role in antigen-induced airway eosinophilia and LAR.
Conformational and structural characteristics of peptides binding to HLA-DR molecules (1991)

A fundamental characteristic of MHC class I and class II proteins is their unusual capacity to form stable complexes with a wide spectrum of peptide ligands. In this study, sets of peptide analogues containing long chain-biotinylated lysine individually substituted for each amino acid in the sequence have been used to explore the structural requirements for the formation of peptide-MHC class II protein complexes. Based on the ability of the analogs to bind both the MHC protein and fluorescent streptavidin, receptor contact residues were identified and from their spacing the conformation of the bound peptides could be inferred. Six separate peptides were studied; three defined by HLA-DR1Dw1-restricted T cells, and three identified by T cells restricted through alleles other than HLA-DR1Dw1. The similar patterns of fluorescent signals observed when the former three peptides were studied indicated that they shared conformational features when bound to HLA-DR1Dw1. In contrast when the latter three peptides were examined, the data indicated that they shared some but not all of the conformational features characteristic of the peptides known to elicit HLA-DR1Dw1-restricted T cells. When the peptide sequences were aligned based on the critical contact residues, two positions of structural homology were apparent. In each sequence, an amino acid with a bulky hydrophobic side chain could be identified separated by four residues from a small amino acid. These minimal structural requirements were consistent with recent experiments demonstrating that only a small number of side chains in the peptide were necessary for binding to the MHC protein.
ChemInform Abstract: HIGHLY STEREOSELECTIVE CONSTRUCTION OF CHIRAL QUATERNARY CARBON: ASYMMETRIC SYNTHESIS OF β,β‐DISUBSTITUTED Γ‐BUTYROLACTONES (1982)

Abstract Durch Verwendung des chiralen Synthons (Ia) gelingt es in der im Formelschema angegebenen Weise durch zwei aufeinanderfolgende Alkylierungen die dialkylierten Derivate (IV) mit einer Isomerenrein‐ 84 heit von 96 ‐ 99% zu erhalten.
NMR structural studies on a nonnatural deoxyribonucleoside which mediates recognition of GC base pairs in pyrimidine.cntdot.purine.cntdot.pyrimidine DNA triplexes (1993)

As a part of our ongoing efforts to define the structural aspects of unusual pairing alignments in DNA triplexes by nuclear magnetic resonance spectroscopy, we have examined the structural role of a nonnatural deoxyribonucleoside, P1, that has been shown to mediate the recognition of GC base pairs in pyrimidine-purine-pyrimidine DNA triplexes [Koh, J.S., & Dervan, P.B. (1992) J. Am. Chem Soc. 114, 1470]. A qualitative interpretation of the NMR data indicates that this analog of protonated cytosine is readily accommodated in the third strand segment of an intramolecular triplex system. Furthermore, the observed NOE patterns position the imino and amino protons of P1 opposite the N7 and O6 atoms of guanine, respectively, consistent with the previously proposed pairing scheme.
White Paper on Adoptive Cell Therapy for Cancer with Tumor-Infiltrating Lymphocytes: A Report of the CTEP Subcommittee on Adoptive Cell Therapy (2011)

Adoptive T-cell therapy (ACT) using expanded autologous tumor-infiltrating lymphocytes (TIL) and tumor antigen-specific T cell expanded from peripheral blood are complex but powerful immunotherapies directed against metastatic melanoma. A number of nonrandomized clinical trials using TIL combined with high-dose interleukin-2 (IL-2) have consistently found clinical response rates of 50% or more in metastatic melanoma patients accompanied by long progression-free survival. Recent studies have also established practical methods for the expansion of TIL from melanoma tumors with high success rates. These results have set the stage for randomized phase II/III clinical trials to determine whether ACT provides benefit in stage IV melanoma. Here, we provide an overview of the current state-of-the art in T-cell-based therapies for melanoma focusing on ACT using expanded TIL and address some of the key unanswered biological and clinical questions in the field. Different phase II/III randomized clinical trial scenarios comparing the efficacy of TIL therapy to high-dose IL-2 alone are described. Finally, we provide a roadmap describing the critical steps required to test TIL therapy in a randomized multicenter setting. We suggest an approach using centralized cell expansion facilities that will receive specimens and ship expanded TIL infusion products to participating centers to ensure maximal yield and product consistency. If successful, this approach will definitively answer the question of whether ACT can enter mainstream treatment for cancer.
The automatic recognition of gestures (1992)

Abstract Gesture-based interfaces, in which the user specifies commands by simple freehand drawings,offer an alternative to traditional keyboard, menu, and direct manipulation interfaces. The ability to specify objects, an operation, and additional parameters with a single intuitive gesture makesgesture-based systems appealing to both novice and experienced users.
Fast self-diffusion of ions in CH3NH3PbI3: the interstiticaly mechanism versus vacancy-assisted mechanism (2016)

The stability of organic–inorganic halide perovskites is a major challenge for their applications and has been extensively studied.
Caspase-8 Promotes Cell Motility and Calpain Activity under Nonapoptotic Conditions (2006)

Significant caspase-8 activity has been found in normal and certain tumor cells, suggesting that caspase-8 possesses an alternative, nonapoptotic function that may contribute to tumor progression. In this article, we report that caspase-8 promotes cell motility. In particular, caspase-8 is required for the optimal activation of calpains, Rac, and lamellipodial assembly. This represents a novel nonapoptotic function of caspase-8 acting at the intersection of the caspase-8 and calpain proteolytic pathways to coordinate cell death versus cell motility signaling.
An elliptical head tracker (2002)

A simple algorithm for tracking a person's head is presented. A two-dimensional model, namely an ellipse, is used to approximate the head's contour. When a new image becomes available, a local search determines the position and size of the best ellipse by maximizing the normalized sum of the image gradient magnitude around the perimeter of the ellipse. The local search begins from a predicted position, using the head's velocity which eliminates the tracker's dependence upon maximum velocity. The tracker operates at 30 Hz and actively controls camera pan and tilt in order to track a person moving in a real environment. The algorithm tolerates full 360-degree rotation of the body as well as moderate amounts of occlusion, and it performs reacquisition of the subject.
Mechanisms of Active Transport in Isolated Bacterial Membrane Vesicles (1973)

Abstract The membrane-bound d-lactate dehydrogenase of Escherichia coli ML 308–225 has been solubilized and purified to homogeneity. The enzyme has a molecular weight of 75,000 ± 7% and contains approximately 1 mole of flavin adenine dinucleotide per mole of enzyme. Its pH optimum before exposure to Triton X-100 is 7.5 to 8.0; after exposure it is 8.0 to 9.5. The purified enzyme has a Km value of 1.4 x 10-3 m for d-lactate and 3.0 x 10-2 m for l-lactate before exposure to Triton X-100; after exposure its Km values are 0.5 x 10-2 m and 2.1 x 10-2 m for the same substrates, respectively. The enzyme is not active with d-glycerate, l-glycerate, succinate, malate, d-tartrate, l-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of d-lactate to pyruvate. The enzyme is not inhibited by p-chloromercuribenzoate, n-ethylmaleimide, or 5,5'-dithiobis(2-nitrobenzoic acid) but is inactivated by treatment with 2-hydroxy-3-butynoic acid.
Differential specificity for binding of retinoblastoma binding protein 2 to RB, p107, and TATA-binding protein. (1994)

The growth suppressor activities of the RB and p107 products are believed to be mediated by the reversible binding of a heterogeneous family of cellular proteins to a conserved T/E1A pocket domain that is present within both proteins. To study the functional role of these interactions, we examined the properties of cellular retinoblastoma binding protein 2 (RBP2) binding to RB, p107, and the related TATA-binding protein (TBP) product. We observed that although RBP2 bound exclusively to the T/E1A pocket of p107, it could interact with RB through independent T/E1A and non-T/E1A domains and with TBP only through the non-T/E1A domain. Consistent with this observation, we found that a mutation within the Leu-X-Cys-X-Glu motif of RBP2 resulted in loss of ability to precipitate p107, while RB- and TBP-binding activities were retained. We located the non-T/E1A binding site of RBP2 on a 15-kDa fragment that is independent from the Leu-X-Cys-X-Glu motif and encodes binding activity for RB and TBP but does not interact with p107. Despite the presence of a non-T/E1A binding site, however, recombinant RBP2 retained the ability to preferentially precipitate active hypophosphorylated RB from whole-cell lysates. In addition, we found that cotransfection of RBP2 can reverse in vivo RB-mediated suppression of E2F activity. These findings confirm the differential binding specificities of the related RB, p107, and TBP proteins and support the presence of multifunctional domains on the nuclear RBP2 product which may allow complex interactions with the cellular transcription machinery.
Soft Chemistry Synthesis and Characterization of Layered Li1-xNi1-yCoyO2-δ (0 ≤ x ≤ 1 and 0 ≤ y ≤ 1) (2001)

Li1-xNi1-yCoyO2-δ oxides have been synthesized for 0 ≤ x ≤ 1 and 0 ≤ y ≤ 1 by chemically extracting lithium at ambient temperature from LiNi1-yCoyO2 with an oxidizing agent NO2PF6 in acetonitrile medium. The samples have been characterized by X-ray diffraction, wet-chemical analyses to determine lithium and oxygen contents, and infrared spectroscopy. While the nickel-rich end members Ni1-yCoyO2-δ have the O3 structure similar to the initial LiNiO2, but with smaller lattice parameters, the cobalt-rich end members Ni1-yCoyO2-δ consist of a mixture of P3 and O1 phases that are formed from the initial O3 structure of LiCoO2 by a sliding of the oxide-ion layers. The nickel-rich phases Li1-xNi1-yCoyO2-δ have an oxygen content close to 2 with a negligible amount of oxygen vacancies, while the cobalt-rich phases have considerable amount of oxygen vacancies (δ = 0.33 in CoO2-δ). Fourier transform infrared spectroscopy indicates a semiconductor to metal transition in Li1-xCoO2-δ for (1 − x) < 0.77. On the other hand, Li1-xNi1-yCoyO2-δ oxides with 0 ≤ y ≤ 0.5 remain as semiconductors for the entire 0 ≤ (1 − x) ≤ 1. The differences in oxygen loss behavior and electrical conduction are explained on the basis of qualitative band diagrams.