gebhart/patrepeval huggingface dataset:
COOL: A Language for Describing Coordination in Multi Agent Systems. (1995)

Agent interaction takes place at several levels. Current work in the ARPA Knowledge Sharing Effort has addressed the information content level by the KIF language and the intentional level by the KQML language. In this paper we address the coordination level by means of our Coordination Language (COOL) that relies on speech act based communication, but integrates it in a structured conversation framework that captures the coordination mechanisms agents use when working together. We are currently using this language (i) to represent coordination mechanisms for the supply chain of manufacturing enterprises modeled as intelligent agents and (ii) as an environment for designing and validating coordination protocols for multi-agent systems. This paper describes the basic elements of this language: conversation objects, conversation rules, error recovery rules, continuation rules, conversation nesting. The actual COOL source code and a running trace for the n-queens problem are presented in ...
The Secretion and Uptake of Lysosomal Phospholipase A2 by Alveolar Macrophages (2008)

Macrophages have long been known to secrete a Phospholipase A(2) with an acidic pH optimum in response to phagocytic stimuli. However, the enzyme or enzymes responsible for this activity have not been identified. We report that mouse alveolar macrophages release lysosomal phospholipase A(2) (LPLA(2)) into the medium of cultured cells following stimulation with zymosan. The release of the enzyme was detected by enzymatic activity assays as well as by Western blotting using an Ab against mouse LPLA(2). LPLA(2) is a high mannose type glycoprotein found in lysosomes, suggesting that the released enzyme might be reincorporated into alveolar macrophages via a mannose or mannose phosphate receptor. Recombinant glycosylated mouse LPLA(2) produced by HEK293 cells was applied to LPLA(2)-deficient (LPLA(2)(-/-)) mouse alveolar macrophages. The uptake of exogenous LPLA(2) into LPLA(2)(-/-) alveolar macrophages occurred in a concentration-dependent manner. The LPLA(2) taken into the alveolar macrophages colocalized with the lysosomal marker, Lamp-1. This uptake was significantly suppressed in the presence of alpha-methyl-mannoside but not in the presence of mannose 6-phosphate. Thus, the predominant pathway for uptake of exogenous LPLA(2) is via the mannose receptor, with subsequent translocation into acidic, Lamp-1-associated compartments. LPLA(2)(-/-) alveolar macrophages are characterized by marked accumulation of phosphatidylcholine and phosphatidylethanolamine. Treatment with the recombinant LPLA(2) rescued the LPLA(2)(-/-) alveolar macrophages by markedly decreasing the phospholipid accumulation. The application of a catalytically inactive LPLA(2) revealed that the enzymatic activity of LPLA(2) was required for the phospholipid reduction. These studies identify LPLA(2) as a high m.w.-secreted Phospholipase A(2).
Timing uncertainty measurements on the Power5 microprocessor (2004)

On-chip timing measurement (Skitter) circuits are included on the Power5 microprocessor. By cross-coupling the 3 Skitter instances, the combined effect of jitter, skew, and supply noise can be measured for all cycles of a pattern or an application. System results show a maximum of 27ps timing impact on a 500ps path.
Microfluidic Device Fabrication by Thermoplastic Hot-Embossing (2012)

Due to their low cost compatibility with replication-based fabrication methods, thermoplastics represent an exceptionally attractive family of materials for the fabrication of lab-on-a-chip platforms. A diverse range of thermoplastic materials suitable for microfluidic fabrication is available, offering a wide selection of mechanical and chemical properties that can be leveraged and further tailored for specific applications. While high-throughput embossing methods such as reel-to-reel processing of thermoplastics is an attractive method for industrial microfluidic chip production, the use of single chip hot embossing is a cost-effective technique for realizing high-quality microfluidic devices during the prototyping stage. Here we describe methods for the replication of microscale features in two thermoplastics, polymethylmethacrylate (PMMA) and polycarbonate (PC), using hot embossing from a silicon template fabricated by deep reactive-ion etching.
Overlap and refractory effects in a brain–computer interface speller based on the visual P300 event-related potential (2009)

We reveal the presence of refractory and overlap effects in the event-related potentials in visual P300 speller datasets, and we show their negative impact on the performance of the system. This finding has important implications for how to encode the letters that can be selected for communication. However, we show that such effects are dependent on stimulus parameters: an alternative stimulus type based on apparent motion suffers less from the refractory effects and leads to an improved letter prediction performance.
An image synthesizer (1985)

We introduce the concept of a Pixel Stream Editor. This forms the basis for an interactive synthesizer for designing highly realistic Computer Generated Imagery. The designer works in an interactive Very High Level programming environment which provides a very fast concept/implement/view iteration cycle.Naturalistic visual complexity is built up by composition of non-linear functions, as opposed to the more conventional texture mapping or growth model algorithms. Powerful primitives are included for creating controlled stochastic effects. We introduce the concept of "solid texture" to the field of CGI.We have used this system to create very convincing representations of clouds, fire, water, stars, marble, wood, rock, soap films and crystal. The algorithms created with this paradigm are generally extremely fast, highly realistic, and asynchronously parallelizable at the pixel level.
Acid binding and detritylation during oligonucleotide synthesis (1996)

Under the conditions normally used for detritylation in oligonucleotide synthesis, the haloacetic acid binds strongly to the oligonucleotide. Acetonitrile also forms a complex with the deblocking acid, in competition with the oligonucleotide, and drastically slows detritylation. Incomplete removal of acetonitrile during the deblock step may slow the kinetics enough to result in incomplete detritylation of the oligonucleotide. Acid binding to the growing oligonucleotide causes striking chromatographic effects in the presence of high oligonucleotide mass densities. In packed-bed column reactors, at low linear velocities, the acid binding almost completely depletes free acid from the deblocking solution. This results in an advancing zone within which the oligonucleotide is saturated with acid. Detritylation occurs mostly in a narrow band at the front of the advancing saturated zone. Increasing the DCA concentration in order to achieve quick saturation can give faster and more complete detritylation while minimizing the exposure time of the oligonucleotide to acid.
Self-organized public-key management for mobile ad hoc networks (2003)

In contrast with conventional networks, mobile ad hoc networks usually do not provide online access to trusted authorities or to centralized servers, and they exhibit frequent partitioning due to link and node failures and to node mobility. For these reasons, traditional security solutions that require online trusted authorities or certificate repositories are not well-suited for securing ad hoc networks. We propose a fully self-organized public-key management system that allows users to generate their public-private key pairs, to issue certificates, and to perform authentication regardless of the network partitions and without any centralized services. Furthermore, our approach does not require any trusted authority, not even in the system initialization phase.
Technologies for Boron Removal (2007)

Boron has been widely used in various manufacturing industries, and the level of boron in the industrial effluent can range from as high as several grams per liter to as low as a few micrograms per liter. Due to the potential adverse health effect of boron on human beings, the World Health Organization (WHO) has provided a guideline for the boron concentration in drinking water to be less than 0.5 mg/L. Therefore, the boron-containing effluents or solutions should be treated before they could be discharged into natural water bodies. This paper critically reviews various technologies used for boron removal, including those recently developed, electrocoagulation (EC) and double-layered hydroxide (DLH) compounds, as well as conventional and advanced treatment technologies such as chemical precipitation, ion exchange, reverse osmosis, adsorption, liquid−liquid extraction, and electrodialysis. Particular focus is given to the comparative performance and advantages and disadvantages of these technologies for boron removal. EC and DLH have been proven to be highly effective for boron removal from solutions with a high boron concentration, while a DLH compound alone can effectively treat solutions with low boron concentrations (<50 mg/L) to the WHO recommended drinking water standard (≤0.5 mg/L). Pilot- and full-scale trials, however, need to be carried out in order to implement EC and DLH compounds for removing boron in a full scale.
Immunosuppression by D-penicillamine in vitro. Inhibition of human T lymphocyte proliferation by copper- or ceruloplasmin-dependent generation of hydrogen peroxide and protection by monocytes. (1984)

It has been suggested that D-penicillamine is active in rheumatoid arthritis because of its capacity to function as a selective inhibitor of T lymphocyte function. The basis for the immunosuppressive action of this drug as well as mechanisms whereby the effect of D-penicillamine could be modified by elements of rheumatoid synovial tissue were examined. As previously reported, D-penicillamine, in the presence of copper ions markedly inhibited mitogen-induced human T lymphocyte DNA synthesis. Since the vast majority of copper in the body exists as an integral part of the ceruloplasmin molecule, the capacity of this cuproprotein to augment D-penicillamine-mediated inhibition of T cell function was examined. The requirement for copper ions could be entirely replaced by purified ceruloplasmin, which had been depleted of nonspecifically bound copper by passage over Chelex-100 columns. The mechanism by which D-penicillamine in the presence of either copper ions or ceruloplasmin caused inhibition of T lymphocyte responsiveness was examined. Partial protection from this inhibitory effect was accomplished by sodium borohydride. While superoxide dismutase had no protective effect, catalase was found to protect lymphocyte responsiveness totally from the inhibitory action of D-penicillamine and either copper ions or ceruloplasmin. Similarly, horseradish peroxidase and myeloperoxidase also protected responsiveness from these inhibitors while boiled catalase was without effect. These results indicate that inhibition of T lymphocyte responsiveness resulted from the generation of hydrogen peroxide. Since a number of cells likely to be present at chronic inflammatory sites, such as mononuclear phagocytes, contain enzymatic mechanisms to degrade hydrogen peroxide, the modulatory influence of these cells on the inhibition of T cell function caused by D-penicillamine and copper was examined. Monocytes, whose function was not suppressed by D-penicillamine and copper, were found to protect T lymphocyte responsiveness from the inhibitory effects of either the mixture of D-penicillamine and CuSO4 or of hydrogen peroxide. By contrast, endothelial cells, fibroblasts, or cells obtained from enzyme-digested noninflamed synovium could not protect T cells from the inhibitory effects of D-penicillamine and copper. Protection of T cells was afforded by means of a heat labile, azide-sensitive soluble factor present in lysates of human monocytes. These results indicate that the mechanism whereby D-penicillamine in the presence of copper or ceruloplasmin inhibits T lymphocyte responsiveness involves the generation of hydrogen peroxide and that other neighboring cells likely to be found w
Staphylococcal Vaccines: A Realistic Dream (1996)

Staphylococcus aureus, especially multidrug resistant strains, continues to be a leading cause of serious nosocomial infections. In spite of the debate among investigators in the field, the discovery of serologically distinct capsular polysaccharides on the surface of clinical isolates has renewed the prospects for development of vaccines and passive protective immunity against S. aureus infections. Capsular polysaccharide conjugate vaccines have now been produced and proven to be safe and immunogenic in both healthy and in a significant percentage of immunocompromised patients. Antibodies generated in humans against these vaccines have been shown to mediate type-specific opsonophagocytosis, and to protect animals against lethal challenge with the appropriate S. aureus isolate.
Fish Oil–Derived Fatty Acids, Docosahexaenoic Acid and Docosapentaenoic Acid, and the Risk of Acute Coronary Events (2000)

Our data provide further confirmation for the concept that fish oil-derived fatty acids reduce the risk of acute coronary events. However, a high mercury content in fish could attenuate this protective effect.
Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor (2007)

mAbs that are sensitive to protein conformation can be helpful in studies of protein structure and function; in particular, mAb fragments are useful reagents in membrane protein crystallization. We immunized mice with the rat 5HT2c serotonin receptor and derived clonal hybridoma cells, which we tested for specific antigen reactivity by using the complementarity of purified protein from bacteria and receptor-embedded mammalian cell membranes. Nine mAbs met our criteria for specificity, affinity, and sensitivity to conformational features. Epitopes were mapped in various additional tests. Five of the nine mAbs have cytoplasmic epitopes, and two of these are sensitive to the ligand state of the receptor. These properties should be useful both for structural analysis and in probes of function.
Breast cancer is associated with loss of the c‐kit oncogene product (1992)

The proto-oncogene c-kit encodes a tyrosine kinase receptor related to the PDGF/CSF-1 receptors. Mutations of this gene result in impairment of hematopoiesis, melanogenesis and gametogenesis. Using monoclonal antibodies to the c-kit gene product, we have analyzed its expression in normal and transformed human tissues. Unexpectedly, the receptor was found to be expressed in normal mammary epithelium. While in benign breast lesions, the c-kit gene product was detected at variable levels in 82% of the instances, in primary tumors, no product could be identified in 87% of the cases. This phenotype is maintained in metastatic foci. These findings were confirmed by paired Northern blot analysis of RNA preparations from normal and tumor tissues. These results demonstrate that the c-kit receptor may also be involved in the growth control of mammary epithelium and that this function may be impaired following malignant transformation and de-differentiation.
CoolSpots (2006)

CoolSpots enable a wireless mobile device to automatically switch between multiple radio interfaces, such as WiFi and Bluetooth, in order to increase battery lifetime. The main contribution of this work is an exploration of the policies that enable a system to switch among these interfaces, each with diverse radio characteristics and different ranges, in order to save power - supported by detailed quantitative measurements. The system and policies do not require any changes to the mobile applications themselves, and changes required to existing infrastructure are minimal. Results are reported for a suite of commonly used applications, such as file transfer, web browsing, and streaming media, across a range of operating conditions. Experimental validation of the CoolSpot system on a mobile research platform shows substantial energy savings: more than a 50% reduction in energy consumption of the wireless subsystem is possible, with an associated increase in the effective battery lifetime.
Modified vaccinia virus Ankara induces moderate activation of human dendritic cells (2004)

Modified vaccinia virus Ankara (MVA) is a highly attenuated strain known to be an effective vaccine vector. Here it is demonstrated that MVA, unlike standard vaccinia virus (VACV) strains, activates monocyte-derived human dendritic cells (DCs) as testified by an increase in surface co-stimulatory molecules and the secretion of pro-inflammatory cytokines. Inhibition of virus gene expression by subjecting MVA to UV light or heat treatment did not alter its ability to activate DCs. On the other hand, standard VACV strains activated DCs if virus gene expression was prevented by prior UV light or heat treatment. These results suggest that MVA or standard VACV particles are responsible for DC activation but, in the case of standard VACV strains, virus gene expression prevents activation. Additional experiments showed that DCs were activated by MVA-infected HeLa cells and, under these conditions, could induce secretion of gamma interferon from T lymphocytes more efficiently than if a replication-competent VACV strain was employed. These data provide one explanation for the remarkable immune-stimulating capacity of MVA in the absence of virus multiplication.
Spin Environment Engineering for a Persistent Current Qubit (2001)

A persistent current qubit has two quantum states with opposite currents flowing in a superconducting loop. Their magnetic field couple to nuclear spins. The qubit state is not only perturbed by the spins but it also gets entangled with the spins' state on a very short timescale. However, when the same spins are exposed to a strong but less than critical external magnetic field, then the qubit field is just a small perturbation on top of the external field and the entanglement with each spin is negligible. For a qubit which is more microscopic than certain threshold this partial entanglement results in negligible partial decoherence.
DNA Immunization byPlasmodium falciparumLiver-Stage Antigen 3 Induces Protection againstPlasmodium yoeliiSporozoite Challenge (2001)

DNA-based immunization of mice by Plasmodium falciparum liver-stage antigen 3 (PfLSA3), a novel highly conserved P. falciparum preerythrocytic antigen, was evaluated. Animals developed a dominant Th1 immune response (high gamma interferon T-cell responses and predominance of immunoglobulin G2a) to each of three recombinant proteins spanning the molecule. We have exploited the immunological cross-reactivity of PfLSA3 with its putative homologue on sporozoites of the rodent parasite Plasmodium yoelii, and we show for the first time that responses induced by PfLSA3 in mice significantly protect against a heterologous challenge by P. yoelii sporozoites. These results support a significant effect of DNA-induced immune responses on preerythrocytic stages.
A study of application performance with non-volatile main memory (2015)

Attaching next-generation non-volatile memories (NVMs) to the main memory bus provides low-latency, byte-addressable access to persistent data that should significantly improve performance for a wide range of storage-intensive workloads. We present an analysis of storage application performance with non-volatile main memory (NVMM) using a hardware NVMM emulator that allows fine-grain tuning of NVMM performance parameters. Our evaluation results show that NVMM improves storage application performance significantly over flash-based SSDs and HDDs. We also compare the performance of applications running on realistic NVMM with the performance of the same applications running on idealized NVMM with the same performance as DRAM. We find that although NVMM is projected to have higher latency and lower bandwidth than DRAM, these difference have only a modest impact on application performance. A much larger drag on NVMM performance is the cost of ensuring data resides safely in the NVMM (rather than the volatile caches) so that applications can make strong guarantees about persistence and consistency. In response, we propose an optimized approach to flushing data from CPU caches that minimizes this cost. Our evaluation shows that this technique significantly improves performance for applications that require strict durability and consistency guarantees over large regions of memory.
Diastereoselective [2+2]-Cycloaddition Reactions of Unsymmetrical Cyclic Ketenes with Imines:  Synthesis of Modified Prolines and Theoretical Study of the Reaction Mechanism (2004)

The synthesis of enantiomerically pure modified proline derivatives was achieved by using spiro beta-lactams as starting material that were prepared in turn by the [2+2]-cycloaddition of unsymmetrical cyclic ketenes with optically active imines. A theoretical study of the [2+2]-cycloaddition reaction, using density-functional methods, gave insights on the origin of the observed stereoselectivity of the Staudinger reaction. The spiro beta-lactams were transformed in the N-Boc derivatives and subjected to nucleophilic ring opening, affording the corresponding enantiomerically pure modified proline derivatives, isolated as orthogonally protected compounds.
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5 (1996)

DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located just upstream of the daunomycin polyketide biosynthesis genes, revealed the presence of six complete genes. The two genes reading right to left include genes encoding the potentially translationally coupled gene products, an acyl carrier protein and a ketoreductase, and the four genes reading divergently, left to right, include two open reading frames of unknown function followed by a gene encoding an apparent glycosyltransferase and dauE, encoding aklaviketone reductase. Extracts of Streptomyces lividans TK24 containing recombinant DauE catalyzed the NADPH-specific conversion of aklaviketone, maggiemycin, and 7-oxodaunomycinone to aklavinone, epsilon-rhodomycinone, and daunomycinone, respectively. Neither the product of dauB nor that of the ketoreductase gene directly downstream of the acyl carrier protein gene demonstrated aklaviketone reductase activity.