gebhart/patrepeval huggingface dataset:
Silica-supported ZnS.cntdot.CdS mixed semiconductor catalysts for photogeneration of hydrogen (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilica-supported ZnS.cntdot.CdS mixed semiconductor catalysts for photogeneration of hydrogenA. Ueno, N. Kakuta, K. H. Park, M. F. Finlayson, A. J. Bard, A. Campion, M. A. Fox, S. E. Webber, and J. M. WhiteCite this: J. Phys. Chem. 1985, 89, 18, 3828–3833Publication Date (Print):August 1, 1985Publication History Published online1 May 2002Published inissue 1 August 1985https://pubs.acs.org/doi/10.1021/j100264a012https://doi.org/10.1021/j100264a012research-articleACS PublicationsRequest reuse permissionsArticle Views342Altmetric-Citations92LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Development of superconducting magnets for beam lines and accelerator at KEK (1981)

Recent rapid development of superconducting magnets at KEK is reported. KEK is now constructing a superconducting pion beam line at the 12GeV Proton Synchrotron. A large bore superconducting dipole and a superconducting septum magnet for the beam line have been manufactured and successfully tested. Concurrently KEK is developing big aperture superconducting accelerator magnet of warm iron and warm bore. The first 1m model dipole of coil I.D. 14cm has been assembled and tested. A dipole field of 5.1 Tesla has been obtained in the second cool down test. This is a test dipole for TRISTAN proton ring magnet. Development program of superconducting magnets at KEK is also presented.
Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain (2010)

Infectious meningitis and encephalitis is caused by invasion of circulating pathogens into the brain. It is unknown how the circulating pathogens dynamically interact with brain endothelium under shear stress, leading to invasion into the brain. Here, using intravital microscopy, we have shown that Cryptococcus neoformans, a yeast pathogen that causes meningoencephalitis, stops suddenly in mouse brain capillaries of a similar or smaller diameter than the organism, in the same manner and with the same kinetics as polystyrene microspheres, without rolling and tethering to the endothelial surface. Trapping of the yeast pathogen in the mouse brain was not affected by viability or known virulence factors. After stopping in the brain, C. neoformans was seen to cross the capillary wall in real time. In contrast to trapping, viability, but not replication, was essential for the organism to cross the brain microvasculature. Using a knockout strain of C. neoformans, we demonstrated that transmigration into the mouse brain is urease dependent. To determine whether this could be amenable to therapy, we used the urease inhibitor flurofamide. Flurofamide ameliorated infection of the mouse brain by reducing transmigration into the brain. Together, these results suggest that C. neoformans is mechanically trapped in the brain capillary, which may not be amenable to pharmacotherapy, but actively transmigrates to the brain parenchyma with contributions from urease, suggesting that a therapeutic strategy aimed at inhibiting this enzyme could help prevent meningitis and encephalitis caused by C. neoformans infection.
Complex I Subunit Gene Therapy With NDUFA6 Ameliorates Neurodegeneration in EAE (2015)

NDUFA6 gene therapy provided long-term suppression of neurodegeneration in the EAE animal model suggesting that it may also ameliorate the mitochondrial dysfunction associated with permanent disability in optic neuritis and MS patients.
“Inside-out” signal transduction inhibited by isolated integrin cytoplasmic domains (1994)

The affinities of integrin alpha beta heterodimers for extracellular ligands are important regulators of cell adhesion. Intracellular signals provoke changes in the integrin extracellular domain resulting in "activation," as manifested by an increase in affinity. Interactions of integrin cytoplasmic domains with intracellular elements may mediate this "inside-out signaling." Here we report that overexpression of chimeras of the cytoplasmic domain of integrin beta 3 or beta 1 subunits, joined to the extracellular and transmembrane domains of the Tac subunit of the interleukin-2 receptor, reduced integrin affinity. In contrast, chimeras containing the cytoplasmic domain of alpha 5 or alpha IIb or of beta 3 bearing a mutation that disrupts inside-out signaling lacked inhibitory activity. These data suggest that limiting quantities of intracellular factors bind to integrin beta 3 and beta 1 cytoplasmic domains to modulate ligand binding affinity. Structural mimics of these domains may provide a novel means to alter cell adhesion.
Replication and transcription sites are colocalized in human cells (1994)

HeLa cells synchronized at different stages of the cell cycle were permeabilized and incubated with analogues of nucleotide triphosphates; then sites of incorporation were immunolabeled with the appropriate fluorescent probes. Confocal microscopy showed that sites of replication and transcription were not diffusely spread throughout nuclei, reflecting the distribution of euchromatin; rather, they were concentrated in 'foci' where many polymerases act together. Transcription foci aggregated as cells progressed towards the G1/S boundary; later they dispersed and became more diffuse. Replication was initiated only at transcription sites; later, when heterochromatin was replicated in enlarged foci, these remained sites of transcription. This illustrates the dynamic nature of nuclear architecture and suggests that transcription may be required for the initiation of DNA synthesis.
Key implication of CD277/butyrophilin-3 (BTN3A) in cellular stress sensing by a major human γδ T-cell subset (2012)

Human peripheral Vγ9Vδ2 T cells are activated by phosphorylated metabolites (phosphoagonists [PAg]) of the mammalian mevalonate or the microbial desoxyxylulose-phosphate pathways accumulated by infected or metabolically distressed cells. The underlying mechanisms are unknown. We show that treatment of nonsusceptible target cells with antibody 20.1 against CD277, a member of the extended B7 superfamily related to butyrophilin, mimics PAg-induced Vγ9Vδ2 T-cell activation and that the Vγ9Vδ2 T-cell receptor is implicated in this effect. Vγ9Vδ2 T-cell activation can be abrogated by exposing susceptible cells (tumor and mycobacteria-infected cells, or aminobisphosphonate-treated cells with up-regulated PAg levels) to antibody 103.2 against CD277. CD277 knockdown and domain-shuffling approaches confirm the key implication of the CD277 isoform BTN3A1 in PAg sensing by Vγ9Vδ2 T cells. Fluorescence recovery after photobleaching (FRAP) experiments support a causal link between intracellular PAg accumulation, decreased BTN3A1 membrane mobility, and ensuing Vγ9Vδ2 T-cell activation. This study demonstrates a novel role played by B7-like molecules in human γδ T-cell antigenic activation and paves the way for new strategies to improve the efficiency of immunotherapies using Vγ9Vδ2 T cells.
Viking: a multi-spanning-tree Ethernet architecture for metropolitan area and cluster networks (2005)

Simplicity, cost effectiveness, scalability, and the economies of scale make Ethernet a popular choice for local area networks, as well as for storage area networks and increasingly metropolitan-area networks. These applications of Ethernet elevate it from a LAN technology to a ubiquitous networking technology, thus prompting a rethinking of some of its architectural features. One weakness of existing Ethernet architecture is its use of single spanning tree, which, while useful at avoiding routing loops, leads to low link utilization and long failure recovery time. To apply Ethernet to cluster networks and MANs, these problems need to be addressed. We propose a multi-spanning-tree Ethernet architecture, called Viking, that improves both aggregate throughput and fault tolerance by exploiting standard virtual LAN technology in a novel way. By supporting multiple spanning trees through VLAN, Viking makes the most of the inherent redundancies in most mesh-like networks and delivers a multi-fold throughput gain over single-spanning-tree Ethernet with the same physical network topology. It also provides much faster failure recovery, reducing the down-time to a sub-second range from that of multiple seconds in single-spanning-tree Ethernet architecture. Finally, based only on standard mechanisms, Viking is readily implementable on commodity Ethernet switches without any firmware modifications.
Subfractionation of Sf 4–105, Sf 4–20 and high density lipoproteins (1972)

Abstract Subfractionation of the total low density S f 4–10 5 , the low density S f 4–20 and high density plasma (or serum) lipoproteins has been accomplished using a cumulative flotation rate procedure. Fractionation employs nonlinear salt gradients and high performance swinging bucket rotors. Subfractionation of the total low density lipoproteins with minimal contamination allows and extremely accurate lipoprotein mass measurement of S f &gt; 400, total very low density lipoproteins and low density lipoproteins (LDL) by elemented CHN analysis. Physical and chemical data on LDL and high density lipoprotein (HDL) subfractions are in general agreement with earlier data. Lower molecular weight data are obtained for HDL subfractions than reported earlier; however this may be the result of the different fractionation procedures used.
Computational Discovery of New Zeolite-Like Materials (2009)

We present a database of computationally predicted zeolite-like materials. The materials were identified by a Monte Carlo search of Si atom positions as the number of unique atoms, density, space group, and unit cell of the crystalline material was systematically explored. Over 2.7M unique structures were identified, with roughly 10% within the +30 kJ/mol Si energetic band above α-quartz in which the known zeolites lie. Predicted structures within this band have geometric and topological characteristics similar to that of the known zeolites. Known zeolites are shown to lie on the low-density edge of the distribution of predicted structures. Dielectric constants and X-ray powder diffraction patterns are calculated. Strategies for chemical synthesis of these materials are discussed, a low-density subset of the materials is identified as particularly interesting, and the complementarity of these materials to high-throughput methods is discussed. These structures have been deposited in two publicly available databases.
African arts between curios, antiquities, and avant-garde at the Maison Brummer, Paris (1908 - 1914) (2015)

Before World War I, Hungarian-born art dealer Joseph Brummer (1883-1947) developed in Paris an extensive trade network through talented salesmanship and brilliant art selections. Helped by his brothers Imre (1889-1928) and Ernest (1891-1964), he promoted original art-forms that were not previously integral to the Western art canon. Juxtaposing works from Africa with art of Medieval Europe, Ancient Greece and Rome, as well as creations by living artists, the Maison Brummer blurred the boundaries that existed between these fields of collecting and was instrumental in awakening the interest of many collectors and museum professionals. Despite the Brummer brothers’ extensive international dealership and the fame of the collections they helped build, only recently has in depth scholarship begun to explore their activities. Using an array of unpublished archival documents and little-examined written accounts, this paper focuses on the early history of the Maison Brummer before its expansion to New York in 1914.
Biomimetic Mineralization of Charged Collagen Matrices: In Vitro and In Vivo Study (2003)

Polyanionic collagen matrix prepared by hydrolysis side chain amides of asparagine and glutamine was mineralized in vivo, without inflammatory response, biodegradation, or resorption, with calcium phosphate deposited in close resemblance to the D-periodicity of collagen fibrils assembly. In vitro results with the same material produced mineralized collagen fibers with a similar morphology and chemical characteristics, suggesting that amide hydrolysis may have introduced into this matrix, signs for the controlled mineralization of collagen fiber. TEM indicated that amide hydrolysis occurred near the OVERLAP and GAP zones, as suggested by the significant reduction in inter-band distances in these regions. The lack of an inflammatory response associated to the similar mineralization pattern observed in vivo and in vitro suggests not only the biomimetic behavior of polyanionic collagen matrix, but also its potential uses as scaffold for bone tissue reconstruction. Based on these results, a model for the in vitro mineralization was also proposed.
Detection of c-Ki-ras mutations in faecal samples from sporadic colorectal cancer patients. (1995)

Colonic exfoliated epithelial cells in faecal material provide a source of human DNA which has been analysed for the presence of the tumour marker ras, in order to detect early tumour cells. The stool samples were subjected to a preliminary sample preparation step followed by centrifugation. DNA was extracted from both the centrifugation pellet and supernatant fractions, as well as from endoscopy washings, using a conventional phenol chloroform extraction method and was then purified on glass milk or spin columns. The purified DNA was amplified using mitochondrial primers and analysed for ras mutations using a non-radioactive, allele specific mismatch method. Corresponding tumour DNA was analysed for mutations using the same method. The results show that approximately 50% of the faecal samples analysed exhibited the presence of ras mutations which were also observed in the corresponding tumours. A double mutation was detected in one supernatant. Our findings represent an important stage in the development of a diagnostic test for the early detection of colorectal cancer.
Dual Targeting of Innate and Adaptive Checkpoints on Tumor Cells Limits Immune Evasion (2018)

CD47 on tumor cells protects from phagocytosis, while PD-L1 dampens T cell-mediated tumor killing. However, whether and how CD47 and PD-L1 coordinate is poorly understood. We reveal that CD47 and PD-L1 on tumor cells coordinately suppress innate and adaptive sensing to evade immune control. Targeted blockade of both CD47 and PD-L1 on tumor cells with a bispecific anti-PD-L1-SIRPα showed significantly enhanced tumor targeting and therapeutic efficacy versus monotherapy. Mechanistically, systemic delivery of the dual-targeting heterodimer significantly increased DNA sensing, DC cross-presentation, and anti-tumor T cell response. In addition, chemotherapy that increases "eat me" signaling further synergizes with the bispecific reagent for better tumor control. Our data indicate that tumor cells evolve to utilize both innate and adaptive checkpoints to evade anti-tumor immune responses and that tumor cell-specific dual-targeting of both checkpoints represents an improved strategy for tumor immunotherapy.
Progress in the Palladium‐Catalyzed Cyanation of Aryl Chlorides (2003)

The development of new palladium catalysts for the cyanation of various aryl and heteroaryl chlorides is described. The combination of amine co-catalysts with chelating phosphine ligands, for example, 1,4-bis(diphenylphosphino)butane (dppb) or 1,5-bis(diphenylphosphino)pentane (dpppe), allows an efficient cyanation of chloroarenes with simple potassium cyanide. General palladium-catalyzed cyanation of nonactivated and deactivated chloroarenes is possible for the first time. Studies of the oxidative addition of aryl halides to palladium triphenylphosphine complexes in the presence and absence of amines suggest that the co-catalyst is capable of preventing catalyst deactivation caused by the presence of excess cyanide ions in solution.
HIV-1 Production from Infected Peripheral Blood T Cells After HTLV-I Induced Mitogenic Stimulation (1988)

The human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type I (HTLV-I) are two distinct human retroviruses that infect T cells. Recent epidemiologic studies have identified a cohort of individuals that are coinfected with both viruses. It is reported here that human peripheral blood leukocytes infected with HIV-1 in vitro can be induced to produce large quantities of HIV-1 after mitogenic stimulation by noninfectious HTLV-I virions. It is also shown that HTLV-I virions may exert this effect prior to, immediately following, or well after the cells are infected with HIV-1. These results provide further impetus for epidemiologic studies of dually infected individuals to determine whether HTLV-I may act as a cofactor for acquired immunodeficiency syndrome (AIDS).
Structure-activity relationships of an exotoxin of Pseudomonas aeruginosa (1977)

The relation of the structure of Pseudomonas aeruginosa exotoxin A (PA toxin) to its enzymatic activity (adenosine 5'-diphosphate-ribosyl transferase) in vitro and to its toxicity in vivo was examined. PA toxin is produced as a single polypeptide chain with a molecular weight of about 71,500. PA toxin is produced by Pseudomonas as a toxic proenzyme that lacks enzymatic activity. Adenosine 5'-diphosphate-ribosyl transferase activity is expressed when the molecule is denatured and reduced or when its is cleaved by Pseudomonas proteases to yield an enzymatically active 27,000-dalton fragment (fragment a). A 45,000-dalton protein is tentatively identified as the enzymatically inactive fragment b of PA toxin. Enzymatically active forms of the toxin lack toxicity for mouse L-cells or mouse lethality. Thus, it is concluded that the native toxin proenzyme is required for toxicity and that a structural rearrangement must precede its intracellular activity.
Two-dimensional signal processing from hexagonal rasters (2005)

Two-dimensional signals are normally sampled and processed as rectangular arrays. For signals which are bandlimited with a circular region of support in the Fourier plane, however, it has been known for some time that a savings in the number of samples required for an exact reconstruction can be realized by sampling the signal on a hexagonal sampling raster. In this presentation we show that a substantial savings in computation can result as well. Included are methods for signal representation, linear system implementation, Fourier transform computation and FIR filter design. Some comparisons between systems defined over rectangular and hexagonal sampling rasters will also be given.
Integrin αvβ3‐targeted therapy for Kaposi's sarcoma with an in vitro‐evolved antibody (2002)

Here, we define integrin alpha(v)beta3 as a molecular target for antibody therapy for Kaposi's sarcoma (KS). We previously reported, using a new phage display strategy based on designed combinatorial V gene libraries, the humanization of mouse monoclonal antibody LM609 directed to human integrin alpha(v)beta3. In the present study, we describe the in vitro affinity maturation of humanized LM609 by using a phage display strategy for the sequential and parallel optimization of three complementarity determining regions of the antibody molecule. The evolved Fab had an affinity of 150 pM and was converted into IgG1 by use of a new mammalian expression vector. The resulting whole antibody, designated JC-7U IgG1, was found to selectively target human KS in a nude mouse model and inhibit tumor growth at a therapeutically relevant dose. Because of its high affinity and its high degree of humanization, JC-7U IgG1 is an excellent drug candidate for therapeutic applications that involve integrin alpha(v)beta3 as the molecular target. Of particular interest is therapy for KS, breast cancer, melanoma, and other cancers in which integrin alpha(v)beta3 is expressed on both angiogenic endothelial cells and tumor cells, which would allow a dual antiangiogenic and antitumor strike with a single drug.
Intracellular expression of single chain antibodies reverts ErbB-2 transformation. (1994)

We report a novel approach for specific in vivo inactivation of the ErbB-2 receptor tyrosine kinase and suppression of ErbB-2-induced transformation. Genes encoding single chain antibodies that specifically bind to the extracellular domain of human ErbB-2 were constructed and expressed intracellularly in NIH/3T3 fibroblasts transformed by activated ErbB-2. The single chain antibodies are derived from monoclonal antibodies FRP5 and FWP51 (Harwerth, I.M., Wels, W., Marte, B. M., and Hynes, N. E. (1992) J. Biol. Chem. 267, 15160-15167) and are composed of heavy and light chain variable domains connected by a flexible peptide linker. The antibodies were provided with: 1) an N-terminal hydrophobic leader sequence to target their synthesis to the lumen of the endoplasmic reticulum, and 2) a C-terminal retention signal to prevent secretion. When expressed in ErbB-2-transformed cells, the single chain antibodies bound to the receptor and prevented its transit through the endoplasmic reticulum. This resulted in the functional inactivation of the receptor and reversion of the transformed phenotype. This is the first demonstration of a targeted and stable inactivation of a cellular oncoprotein via intracellular antibody expression. The use of such a strategy represents a simple and powerful approach to study the in vivo function of receptors and other cellular proteins.
Role of 5-HT receptors in treatment of overactive bladder (2003)

Normal bladder function requires coordinated detrusor relaxation and urethral sphincter contraction during the filling phase and the reverse during micturition. This is achieved by the integration of excitatory, inhibitory, and sensory nerve activity in control centers in the spinal cord, pons, and forebrain. It is possible that much of bladder pathology is related to disturbances in the vesical ganglia, sensory reflex loops, and central control of micturition. Experimental studies in animals have revealed that spinal reflex circuits involved in voiding function exhibit a dense serotonergic innervation, multiple 5-HT receptors, and sensitivity to 5-HT receptor agonists and antagonists, and 5-HT reuptake inhibitors. Activity in the serotonergic pathway generally enhances urine storage by facilitating the vesical sympathetic reflex pathway and inhibiting the parasympathetic voiding pathway. Thus, 5-HT receptor antagonists and reuptake inhibitors represent important targets for the development of new treatments of detrusor overactivity and urinary incontinence. Among the drugs modulating the bladder function through 5HT receptor, duloxetine, a potent and selective inhibitor of 5-HT and NE reuptake, is presently undergoing clinical trials for stress urinary incontinence after being shown to be effective in animal models of this condition.