gebhart/patrepeval huggingface dataset:
Modulation of human neuronal α1E-type calcium channel by α2δ-subunit (1998)

Calcium channels are composed of a pore-forming subunit, alpha 1, and at least two auxiliary subunits, beta- and alpha 2 delta-subunits. It is well known that beta-subunits regulate most of the properties of the channel. The function of alpha 2 delta-subunit is less understood. In this study, the effects of the calcium channel alpha 2 delta-subunit on the neuronal alpha 1E voltage-gated calcium channel expressed in Xenopus oocytes was investigated without and with simultaneous coexpression of either the beta 1b- or the beta 2a-subunit. Most aspects of alpha 1E function were affected by alpha 2 delta. Thus alpha 2 delta caused a shift in the current-voltage and conductance-voltage curves toward more positive potentials and accelerated activation, deactivation, and the installation of the inactivation process. In addition, the efficiency with which charge movement is coupled to pore opening assessed by determining ratios of limiting conductance to limiting charge movement was decreased by alpha 2 delta by factors that ranged from 1.6 (P < 0.01) for alpha 1E-channels to 3.0 (P < 0.005) for alpha 1E beta 1b-channels. These results indicate that alpha 2 delta facilitates the expression and the maturation of alpha 1E-channels and converts these channels into molecules responding more rapidly to voltage.
Phase Locked Loops (1993)

Elementary study of the PLL. Methods of analysis of the linear PLL. Response to pulse signals. Response to sinusoidal signals. Acquisition. Noise. Linear PLL bipolar integrated circuits. Semi-digital PLLs. Applications. Appendix.
The dynamic properties of intermediate filaments during organelle transport (2009)

Intermediate filament (IF) dynamics during organelle transport and their role in organelle movement were studied using Xenopus laevis melanophores. In these cells, pigment granules (melanosomes) move along microtubules and microfilaments, toward and away from the cell periphery in response to alpha-melanocyte stimulating hormone (alpha-MSH) and melatonin, respectively. In this study we show that melanophores possess a complex network of vimentin IFs which interact with melanosomes. IFs form an intricate, honeycomb-like network that form cages surrounding individual and small clusters of melanosomes, both when they are aggregated and dispersed. Purified melanosome preparations contain a substantial amount of vimentin, suggesting that melanosomes bind to IFs. Analyses of individual melanosome movements in cells with disrupted IF networks show increased movement of granules in both anterograde and retrograde directions, further supporting the notion of a melanosome-IF interaction. Live imaging reveals that IFs, in turn, become highly flexible as melanosomes disperse in response to alpha-MSH. During the height of dispersion there is a marked increase in the rate of fluorescence recovery after photobleaching of GFP-vimentin IFs and an increase in vimentin solubility. These results reveal a dynamic interaction between membrane bound pigment granules and IFs and suggest a role for IFs as modulators of granule movement.
Adventitial Expression of Recombinant eNOS Gene Restores NO Production in Arteries Without Endothelium (1998)

The current study was designed to determine the effect of recombinant endothelial nitric oxide synthase (eNOS) gene expression on endothelium-dependent relaxations to bradykinin in isolated canine basilar, coronary, or femoral arteries. Arterial rings were exposed ex vivo (30 minutes at 37 degrees C) to an adenoviral vector encoding either the eNOS gene (AdCMVeNOS) or the beta-galactosidase reporter gene (AdCMVbeta-Gal). Twenty-four hours after transduction, transgene expression was evident mainly in the adventitia. Expression of recombinant proteins was much higher in basilar arteries than in coronary or femoral arteries. Rings of control, AdCMVbeta-Gal, and AdCMVeNOS arteries with and without endothelium were suspended for isometric tension recording. Levels of cGMP were measured by radioimmunoassay. In AdCMVeNOS basilar arteries with endothelium, relaxations to low concentrations of bradykinin (3 x 10(-11) to 10(-9) mol/L) were significantly augmented. In contrast, in coronary and femoral arteries with endothelium, AdCMVeNOS transduction did not affect relaxations to bradykinin. Removal of the endothelium abolished bradykinin-induced relaxations in control and AdCMVbeta-Gal basilar arteries. However, in basilar arteries transduced with AdCMVeNOS even when the endothelium was removed, stimulation with bradykinin (3 x 10(-11) to 10(-9) mol/L) caused relaxations as well as increases in cGMP production. The relaxations to bradykinin were completely blocked by an NOS inhibitor, NG-nitro-L-arginine methyl ester. Electron microscopic analysis revealed that recombinant eNOS protein was expressed in fibroblasts of the basilar artery adventitia. These results suggest that genetically modified adventitial fibroblasts may restore production of NO in cerebral arteries without endothelium. Our findings support a novel concept in vascular biology that fibroblasts in the adventitia may play a role in the regulation of vascular tone after successful transfer and expression of recombinant eNOS gene.
Docker for Data Science (2017)

Learn Docker "infrastructure as code" technology to define a system for performing standard but non-trivial data tasks on medium- to large-scale data sets, using Jupyter as the master controller. It is not uncommon for a real-world data set to fail to be easily managed. The set may not fit well into access memory or may require prohibitively long processing. These are significant challenges to skilled software engineers and they can render the standard Jupyter system unusable.Â As a solution to this problem, Docker for Data ScienceÂ proposes using Docker. You will learn how to useÂ existing pre-compiled public images created by the major open-source technologiesâPython, Jupyter, Postgresâas well as using the Dockerfile to extend these images to suit your specific purposes. The Docker-Compose technology is examined and you will learn how it can be used to build a linked system with Python churning data behind the scenes and Jupyter managing these background tasks. Best practices in using existing images are explored as well as developing your own images to deploy state-of-the-art machine learning and optimization algorithms. Â What Â You'll Learn: Master interactive development using the Jupyter platform Run and build Docker containers from scratch and from publicly available open-source images Write infrastructure as code using the docker-compose tool and its docker-compose.yml file type Deploy a multi-service data science application across a cloud-based system.
Multiple overlapping homologies between two rheumatoid antigens and immunosuppressive viruses. (1991)

Amino acid (aa) sequence homologies between viruses and autoimmune nuclear antigens are suggestive of viral involvement in disorders such as systemic lupus erythematosus (SLE) and scleroderma. We analyzed the frequency of exact homologies of greater than or equal to 5 aa between 61 viral proteins (19,827 aa), 8 nuclear antigens (3813 aa), and 41 control proteins (11,743 aa). Both pentamer and hexamer homologies between control proteins and viruses are unexpectedly abundant, with hexamer matches occurring in 1 of 3 control proteins (or once every 769 aa). However, 2 nuclear antigens, the SLE-associated 70-kDa antigen and the scleroderma-associated CENP-B protein, are highly unusual in containing multiple homologies to a group of synergizing immunosuppressive viruses. Two viruses, herpes simplex virus 1 (HSV-1) and human immunodeficiency virus 1 (HIV-1), contain sequences exactly duplicated at 15 sites in the 70-kDa antigen and at 10 sites in CENP-B protein. The immediate-early (IE) protein of HSV-1, which activates HIV-1 regulatory functions, contains three homologies to the 70-kDa antigen (two hexamers and a pentamer) and two to CENP-B (a hexamer and pentamer). There are four homologies (including a hexamer) common to the 70-kDa antigen and Epstein-Barr virus, and three homologies (including two hexamers) common to CENP-B and cytomegalovirus. The majority of homologies in both nuclear antigens are clustered in highly charged C-terminal domains containing epitopes for human autoantibodies. Furthermore, most homologies have a contiguous or overlapping distribution, thereby creating a high density of potential epitopes. In addition to the exact homologies tabulated, motifs of matching sequences are repeated frequently in these domains. Our analysis suggests that coexpression of heterologous viruses having common immunosuppressive functions may generate autoantibodies cross-reacting with certain nuclear proteins.
N-Terminal analogs of cecropin A: synthesis, antibacterial activity, and conformational properties (1985)

Six analogues of the 37-residue antibacterial peptide cecropin A were synthesized by the solid-phase method: cecropin A-(2-37), [Glu2]cecropin A, [Pro4]cecropin A, [Glu6]cecropin A, [Leu6]cecropin A, and [Pro8]cecropin A. Their antibacterial activities against four test organisms were determined and related to conformational changes observed in their CD spectra and were discussed on the basis of a previously proposed amphipathic alpha-helix model. An aromatic residue in position 2 was shown to be important for activity against all tested bacteria. The highly alpha-helical 1-11 region of cecropin A did not appear to play a significant role in its activity against Escherichia coli but was clearly involved in its interaction against Pseudomonas aeruginosa, Bacillus megaterium, and Micrococcus luteus.
Rethinking Imperialism and Resistance in West Africa: Historiographic Connections for the Classroom (2010)

Even in the postcolonial era, West African history remains plagued by Eurocentric myths and media-driven stereotypes. Though specialists have been struggling with this problem for decades, a rift remains between the elite world of academia and the African history being taught in American schools. In an attempt to bridge this gap, this essay provides a case study and a list of suggested resources designed to help nonspecialist world history teachers rethink European colonial power and its impact on our conception of African history. Through its examination of how West African responses to imperialism interacted with, adapted to, and were ultimately conditioned by European power structures, this essay touches on a range of topics, including pre-colonial African history; the partition of Africa; decolonization; linkages between slavery, colonial labor coercion, and the spread of capitalism; and the continuing impact of colonial rule on present-day conflict in the postcolonial African states.
Traffic Sign Recognition for Intelligent Vehicle/Driver Assistance System Using Neural Network on OpenCV (2007)

Traffic Sign Recognition (TSR) is used to regulate traffic signs, warn a driver, and command or prohibit certain actions. A fast real-time and robust automatic traffic sign detection and recognition can support and disburden the driver and significantly increase driving safety and comfort. Automatic recognition of traffic signs is also important for automated intelligent driving vehicle or driver assistance systems. This paper presents a study to recognize traffic sign patterns using Neural Networks technique. The images are pre-processed with several image processing techniques, such as, threshold techniques, Gaussian filter, Canny edge detection, Contour and Fit Ellipse. Then, the Neural Networks stages are performed to recognize the traffic sign patterns. The system is trained and validated to find the best network architecture. The experimental results show the highly accurate classifications of traffic sign patterns with complex background images and the computational cost of the proposed method.
Performance assessment of bio-inspired systems: flow sensing MEMS hairs (2014)

Despite vigorous growth in biomimetic design, the performance of man-made devices relative to their natural templates is still seldom quantified, a procedure which would however significantly increase the rigour of the biomimetic approach. We applied the ubiquitous engineering concept of a figure of merit (FoM) to MEMS flow sensors inspired by cricket filiform hairs. A well known mechanical model of a hair is refined and tailored to this task. Five criteria of varying importance in the biological and engineering fields are computed: responsivity, power transfer, power efficiency, response time and detection threshold. We selected the metrics response time and detection threshold for building the FoM to capture the performance in a single number. Crickets outperform actual MEMS on all criteria for a large range of flow frequencies. Our approach enables us to propose several improvements for MEMS hair-sensor design.
Endothelial progenitor cells mobilization after maximal exercise according to heart failure severity (2020)

Our study has shown an increased EPCs and circulating endothelial cells mobilization after maximal exercise in CHF patients, but this increase was not associated with syndrome severity. Further investigation, however, is needed.
Phosphorylation of Titin Modulates Passive Stiffness of Cardiac Muscle in a Titin Isoform-dependent Manner (2005)

We investigated the effect of protein kinase A (PKA) on passive force in skinned cardiac tissues that express different isoforms of titin, i.e., stiff (N2B) and more compliant (N2BA) titins, at different levels. We used rat ventricular (RV), bovine left ventricular (BLV), and bovine left atrial (BLA) muscles (passive force: RV > BLV > BLA, with the ratio of N2B to N2BA titin, approximately 90:10, approximately 40:60, and approximately 10:90%, respectively) and found that N2B and N2BA isoforms can both be phosphorylated by PKA. Under the relaxed condition, sarcomere length was increased and then held constant for 30 min and the peak passive force, stress-relaxation, and steady-state passive force were determined. Following PKA treatment, passive force was significantly decreased in all muscle types with the effect greatest in RV, lowest in BLA, and intermediate in BLV. Fitting the stress-relaxation data to the sum of three exponential decay functions revealed that PKA blunts the magnitude of stress-relaxation and accelerates its time constants. To investigate whether or not PKA-induced decreases in passive force result from possible alteration of titin-thin filament interaction (e.g., via troponin I phosphorylation), we conducted the same experiments using RV preparations that had been treated with gelsolin to extract thin filaments. PKA decreased passive force in gelsolin-treated RV preparations with a magnitude similar to that observed in control preparations. PKA was also found to decrease restoring force in skinned ventricular myocytes of the rat that had been shortened to below the slack length. Finally, we investigated the effect of the beta-adrenergic receptor agonist isoprenaline on diastolic force in intact rat ventricular trabeculae. We found that isoprenaline phosphorylated titin and that it reduced diastolic force to a degree similar to that found in skinned RV preparations. Taken together, these results suggest that during beta-adrenergic stimulation, PKA increases ventricular compliance in a titin isoform-dependent manner.
Design and fabrication of a microfluidic circuitboard (1999)

This paper reports the design and fabrication of a micromachined microfluidic circuitboard. The circuitboard consists of a Pyrex wafer in which trenches and connection holes are etched. Channels are then formed by anodically bonding a silicon wafer to the Pyrex wafer. On top of this, various microfluidic devices can be mounted via the anodic bonding technique. This allows a simple way of mass production of different microfluidic systems. To realize other microfluidic systems only the mask layout for creating the channels in the Pyrex wafer has to be changed. The microfluidic circuitboard has been successfully fabricated and single devices have been surface mounted. A whole system has been tested and it proved to be functional and without any leakage.
Primary structure of helodermin, a VIP‐secretin‐like peptide isolated from Gila monster venom (1984)

The complete amino acid sequence of helodermin isolated from the venom of Gila monster was elucidated. The peptide was shown to be a basic pentatriacontapeptide amide: His-Ser-Asp-Ala-Ile-Phe-Thr-Gln-Gln-Tyr-Ser-Lys-Leu-Leu-Ala-Lys-Leu-Ala- Leu-Gln-Lys- Tyr-Leu-Ala-Ser-Ile-Leu-Gly-Ser-Arg-Thr-Ser-Pro-Pro-Pro-NH2. A high degree of sequence similarities to secretin/VIP/PHI/(PHM)/GRF from mammal and bird was observed over the entire N-terminal 1-27 sequence. In particular, the amino acid residues in positions 3, 6 and 7 were found to be common to 9 peptides of the family. Another interesting feature of the structure of helodermin was its C-terminal -Pro-Pro-Pro-NH2 sequence. Isolation of helodermin was the first demonstration of the existence of a secretin/VIP-related peptide in an animal that is neither mammal nor bird.
Octreotide Enhances Portal Pressure Reduction Induced by Propranolol in Cirrhosis: A Randomized, Controlled Trial (2007)

Octreotide enhances HVPG reduction induced by propranolol in cirrhotic patients.
Glycerol kinase: substrate specificity (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGlycerol kinase: substrate specificityDebbie C. Crans and George M. WhitesidesCite this: J. Am. Chem. Soc. 1985, 107, 24, 7008–7018Publication Date (Print):November 1, 1985Publication History Published online1 May 2002Published inissue 1 November 1985https://pubs.acs.org/doi/10.1021/ja00310a044https://doi.org/10.1021/ja00310a044research-articleACS PublicationsRequest reuse permissionsArticle Views933Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Automatic viewing control for 3D direct manipulation (1992)

This paper describes a technique for augmenting the process of 3D direct manipulation by automaticallyfinding an effective placement for the virtual camera. Many of the best techniques for direct manipulation of 3D geometric objects are sensitive to the angle of view, and can thus require that the user coordinate the placement of the viewpoint during the manipulation process. In some cases, this process can be automated. This means that the system can automatically avoid degenerate situations in which translations and rotations are difficult to perform. The system can also select viewpoints and viewing angles which make the object being manipulated visible, ensuring that it is not obstructed by other objects.
The Cutaneous Microcirculation: Ultrastructure and Microanatomical Organization (1997)

The cutaneous microcirculation is organized as two horizontal plexuses. One is situated 1-1.5 mm below the skin surface, and the other is at the dermal-subcutaneous junction. Ascending arterioles and descending venules are paired as they connect the two plexuses. From the upper layer, arterial capillaries arise to form the dermal papillary loops that represent the nutritive component of the skin circulation. There are sphincter-like smooth muscle cells at the point where the ascending arterioles divide to form the arteriolar component of the upper horizontal plexus. At the dermal subcutaneous junction, there are collecting veins with 2-cusped valves that are oriented to prevent the retrograde flow of blood. Laser Doppler flowmetry (LDF) has demonstrated vasomotion of red cell flux localized to the sites of ascending arterioles. The simultaneous recording by LDF of red cell flux and the concentration of moving red blood cells from individual sites allows one to construct by computer topographic maps of these two valves. The two maps, based on initial studies using correlative skin biopsy specimens, can define 1-mm3 volumes of skin that are predominantly arteriolar in composition, predominantly venular in composition, or essentially devoid of all microvascular elements. The electron and light microscopic features that define the microvascular segments, when coupled with the ability of LDF to define the predominant microvascular segments under the probe, will allow one to study both the mechanisms of normal physiological states and the pathogenetic mechanisms underlying pathological skin disorders in which the microvasculature plays a predominant role.
Gadolinium as a CT Contrast Agent: Assessment in a Porcine Model (1999)

Gadolinium produces good vascular enhancement, adequate renal enhancement, and suboptimal hepatic enhancement. Further study is needed to determine the safety of the gadolinium dose required to produce similar enhancement in patients.
Applications of Hilbert–Huang transform to non‐stationary financial time series analysis (2003)

Abstract A new method, the Hilbert–Huang Transform (HHT), developed initially for natural and engineering sciences has now been applied to financial data. The HHT method is specially developed for analysing non‐linear and non‐stationary data. The method consists of two parts: (1) the empirical mode decomposition (EMD), and (2) the Hilbert spectral analysis. The key part of the method is the first step, the EMD, with which any complicated data set can be decomposed into a finite and often small number of intrinsic mode functions (IMF). An IMF is defined here as any function having the same number of zero‐crossing and extrema, and also having symmetric envelopes defined by the local maxima, and minima respectively. The IMF also thus admits well‐behaved Hilbert transforms. This decomposition method is adaptive, and, therefore, highly efficient. Since the decomposition is based on the local characteristic time scale of the data, it is applicable to non‐linear and non‐stationary processes. With the Hilbert transform, the IMF yield instantaneous frequencies as functions of time that give sharp identifications of imbedded structures. The final presentation of the results is an energy–frequency–time distribution, which we designate as the Hilbert Spectrum. Comparisons with Wavelet and Fourier analyses show the new method offers much better temporal and frequency resolutions. The EMD is also useful as a filter to extract variability of different scales. In the present application, HHT has been used to examine the changeability of the market, as a measure of volatility of the market. Published in 2003 by John Wiley &amp; Sons, Ltd.