gebhart/patrepeval huggingface dataset:
Direct Synthesis of β-Mannopyranosides by the Sulfoxide Method (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDirect Synthesis of β-Mannopyranosides by the Sulfoxide MethodDavid Crich and Sanxing SunView Author Information Department of Chemistry, University of Illinois at Chicago, 845 West Taylor Street, Room 4500, Chicago, Illinois 60607-7061 Cite this: J. Org. Chem. 1997, 62, 5, 1198–1199Publication Date (Web):March 7, 1997Publication History Received16 December 1996Published online7 March 1997Published inissue 1 March 1997https://pubs.acs.org/doi/10.1021/jo962345zhttps://doi.org/10.1021/jo962345zrapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views2768Altmetric-Citations227LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cations,Ethers,Free radicals,Oxides,Solvents Get e-Alerts
Therapeutic efficacy of selective nerve root blocks in the treatment of lumbar radicular leg pain (2001)

The objective of this study was to investigate the clinical effectiveness of nerve root blocks (i.e., periradicular injection of bupivacaine and triamcinolone) for lumbar monoradiculopathy in patients with a mild neurological deficit. We have retrospectively analysed 30 patients (29-82 years) with a minor sensory/motor deficit and an unequivocal MRI finding (20 disc herniations, 10 foraminal stenoses) treated with a selective nerve root block. Based on the clinical and imaging findings, surgery (decompression of the nerve root) was justifiable in all cases. Twenty-six patients (87%) had rapid (1-4 days) and substantial regression of pain, five required a repeat injection. 60% of the patients with disc herniation or foraminal stenosis had permanent resolution of pain, so that an operation was avoided over an average of 16 months (6-23 months) follow-up. Nerve root blocks are very effective in the non-operative treatment of minor monoradiculopathy and should be recommended as the initial treatment of choice for this condition.
Characterization of a putative periplasmic transport system for octopine accumulation encoded by Agrobacterium tumefaciens Ti plasmid pTiA6 (1991)

Neoplastic crown gall tumors incited by Agrobacterium tumefaciens release novel amino acid or sugar derivatives known as opines, whose synthesis is directed by genes transferred to plant cells. Agrobacterium cells can transport and catabolize these compounds as sources of carbon and nitrogen. This article describes a region of the pTiA6 plasmid which is required for catabolism of the opine octopine and whose transcription is induced by octopine. This region of the plasmid contains four open reading frames, occQ, occM, occP, and occJ, which show homology to the family of so-called shock-sensitive permeases. TnphoA mutagenesis demonstrated that the OccJ and OccM proteins lie fully or partly in the periplasmic space. The OccJ protein was identified by electrophoresis and found to be fully localized in the periplasmic space. When these proteins were expressed in Escherichia coli, radiolabeled octopine became cell-associated.
A new storage and retrieval method to support editing operations in a multi-disk-based video server (2002)

Since the storage ordering is assumed the same as the retrieval ordering in all the previous storage placement strategies, there is a requirement of a large amount of file copying or merging operations when users insert/delete some data into/from the media data. The observation is also important for video applications because features, like video editing, require the support of random access on individual video frames. We propose a new strategy for the insert/delete operations on continuous media that are striped into multi-disks without reorganization of the whole data. From our performance analysis and simulation, the proposed algorithms for insertion/deletion may require only some additional movement cost.
Sentry tag: an efficient filter scheme for low power cache (2002)

In this paper, we focus on low power design at the architecture level and propose a new architecture, called sentry tag, for reducing the on-chip cache power consumption in processors. The many unnecessary activities in the conventional cache-access process motivate our proposal. By pre-comparing the sentry bit of the access address with the sentry tag before starting a normal cache access, we can filter out and eliminate most unnecessary activities. Thus the power consumption can be effectively reduced. Our method is valuable for both a conventional cache with parallel access and a phased cache with serial access. We used SimpleScalar, a trace-driven simulator, to evaluate the performance of our proposed architecture. Compared to a conventional cache, experimental results from SPEC95 benchmarks show that the additional sentry tag can reduce many unnecessary activities (and hence the power consumption), while the hardware overhead is insignificant. For example, an 8-KB, 4-way data cache with two-bit sentry tag can improve the power consumption by 35% compared to a conventional set-associative cache. The maximum power saving achieved using this architecture depends on the cache configuration, which suggests that the proposed sentry tag is a viable choice for data caches with higher associativity.
Identification of Amino Acid Residues Essential for von Willebrand Factor Binding to Platelet Glycoprotein Ib. (1995)

At sites of vascular injury, von Willebrand factor (VWF) mediates platelet adhesion through binding to platelet glycoprotein Ib (GPIb). The VWF-GPIb interaction was investigated by clustered charged-to-alanine scanning mutagenesis of VWF domain A1 between His-473 and Gly-716. Recombinant variants of VWF were assayed for binding to conformation-dependent monoclonal antibody NMC-4, for ristocetin-induced and botrocetin-induced binding to platelets, and for direct binding to botrocetin. Substitutions at 32 amino acids had no effect on VWF function. The epitope of NMC-4 depended on charged residues between Asp-514 and Arg-632 and not on segments previously implicated by peptide inhibition studies, Cys-474-Pro-488 and Leu-694-Pro-708. Substitutions at Glu-626 and in the segment Asp-520-Lys-534 abolished ristocetin-induced binding of VWF to GPIb but did not affect botrocetin-induced binding, suggesting that these regions are required for modulation by ristocetin but not for binding of VWF to GPIb. Mutations at Glu-596 and Lys-599 decreased binding of VWF to GPIb without affecting its binding to botrocetin, suggesting that this segment interacts directly with GPIb. Alanine substitutions at Arg-545 and in the segments Glu-497-Arg-511 and Arg-687-Glu-689 caused increased binding of VWF to GPIb. These results, and the locations of von Willebrand disease type 2B mutations, suggest that two acidic regions containing the Cys-509-Cys-695 disulfide (Glu-497-Arg-511, Arg-687-Val-698) and one predominantly basic region (Met-540-Arg-578) cooperate to inhibit a distinct GPIb binding site in the VWF A1 domain. This inhibition is relieved by specific mutations, by the modulators ristocetin and botrocetin, or by binding to subendothelial connective tissue.
Cytokines, fibrosis and the failure of glaucoma filtration surgery (1991)

Current therapies for the prevention of fibrosis after glaucoma filtering surgery can be effective but often produce unwanted side effects. An understanding of the cellular basis of the fibrotic reaction may lead to better treatments. Wound repair revolves around angiogenesis and the activation of fibroblasts by cytokines. These peptides, a number of which have been described, act together in intricately complicated networks to encourage fibroblast chemotaxis, proliferation and contractility, as well as to stimulate the production of glycosaminoglycans and collagen. Since interferons seem to inhibit many of these responses, they deserve further evaluation in the treatment of ocular fibrosis.
Determination and Modeling of Kinetics of Cancer Cell Killing by Doxorubicin and Doxorubicin Encapsulated in Targeted Liposomes (2004)

Various mathematical approaches have been devised to relate the cytotoxic effect of drugs in cell culture to the drug concentration added to the cell culture medium. Such approaches can satisfactorily account for drug response when the drugs are free in solution, but the approach becomes problematic when the drug is delivered in a drug delivery system, such as a liposome. To address this problem, we have developed a simple model that assumes that the cytotoxic potency of a drug is a function of the intracellular drug level in a critical compartment. Upon exposure to drug, cell death commences after a lag time, and the cell kill rate is dependent on the amount of drug in the critical intracellular compartment. The computed number of cells in culture, at any time after exposure to the drug, takes into account the cell proliferation rate, the cell kill rate, the average intracellular drug concentration, and a lag time for cell killing. We have applied this model to compare the cytotoxic effect of doxorubicin (DOX), or DOX encapsulated in a liposome that is targeted to CD44 on B16F10 melanoma cells in culture. CD44 is the surface receptor that binds to hyaluronan and is overexpressed on various cancer cells, including B16F10. We have shown previously that the drug encapsulated in hyaluronan-targeted liposomes was more potent than was the free drug. The model required the determination of the cell-associated DOX after the cells were incubated with various concentrations of the free or the encapsulated drug for 3 h, and the quantification of cell number at various times after exposure to the drug. The uptake of encapsulated drug was greater than that of the free drug, and the ratio of cell association of encapsulated:free drug was 1.3 at 0.5 micro g/ml and increased to 3.3 at 20 micro g/ml DOX. The results demonstrate that the enhanced potency of the encapsulated drug could stem from its enhanced uptake. However, in certain cases, where larger amounts of the free drug were added, such that the intracellular amounts of drug exceeded those obtained from the encapsulated drug, the numbers of viable cells were still significantly smaller for the encapsulated drug. This finding demonstrates that for given amounts of intracellular DOX, the encapsulated form was more efficient in killing B16F10 cells than the free drug. The outcome was expressed in the kinetic model as a 5-6-fold larger rate constant of cell killing potency for the encapsulated drug versus the free drug. The model provides a quantitative framework for comparing the cytotoxic effect in cultured cells when applying the drug in the free form or in a delivery system.
A robust denoising method for random-valued impulse noise based on smooth and texture region separation (2012)

So far it is still difficult to remove random-valued impulse noise excellently at any noise level for any image. In this paper, we present a novel robust two-phase denoising scheme based on smooth and texture region separation. In the first phase, all pixels are classified into two groups by using cascade window filtering: the smooth region pixels and the texture region pixels. Then we identify pixels which are likely to be contaminated by noise in these two groups separately. In the second phase, detail-preserving regularization [13] is used to restore the image. Extensive simulations demonstrate the robustness of this proposed approach and it can significantly outperform typical state-of-the-art denoising methods generally, especially for images corrupted by severe noise.
Two types of novel dipeptidyl aminopeptidases from Pseudomonas sp. strain WO24 (1996)

Two kinds of dipeptidyl aminopeptidase I (DAP I [cathepsin C])-like activities which hydrolyze Gly-Phe-p-nitroanilide (Gly-Phe-pNA) were detected in Pseudomonas sp. strain WO24. They were purified and characterized. The isolated enzymes, named DAP BII and DAP BIII, were revealed to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing. DAP BII was estimated to have a molecular mass of 150,000 Da by gel filtration and a subunit size of 73,000 Da by SDS-PAGE, indicating it to be a homodimer. The molecular mass of DAP BIII was evaluated to be approximately 60,000 Da by gel filtration and 69,000 Da by SDS-PAGE, indicating that it is monomeric. The isoelectric points of DAP BII and DAP BIII were 6.1 and 5.0, and their optimal pHs were 8.0 and 8.5 to 9.0, respectively. The result of peptide mapping for DAP BII and DAP BIII showed that these enzymes consist of different components. Both enzymes were completely inhibited by diisopropylphosphofluoride but not by general thiol inhibitors, indicating that they are serine proteases. DAP BII and DAP BIII hydrolyzed Gly-Phe-pNA but not Gly-Arg-pNA, both of which are model substrates for mammalian DAP I. Despite these shared activities toward DAP I, DAP BII released dipeptides from Ala-Ala-pNA and Lys-Ala-4-methylcoumarinamide (a substrate for DAP II), whereas DAP BIII did not hydrolyze either of these compounds and was presumed to prefer substrates composed of bulky, hydrophobic amino acids at P1 and P1' positions. In addition, DAP BII showed no endopeptidase activity, whereas DAP BIII possessed the activity on N-terminally blocked peptide derivatives besides exopeptidase activity. Assays performed with bioactive peptides such as angiotensin I and neuromedin N as substrates indicate that DAP BII has a considerably broader substrate specificity than DAP BIII and is able to hydrolyze an X-Pro bond, an imido bond that few peptidases and no known DAPs can cleave. These characteristics, namely, substrate specificities, molecular mass, pI, peptide mapping, pH optimum, and effect of inhibitors, suggested that the two DAPs purified in this work are distinct enzymes and do not belong to any of the previously reported DAP classes.
The NH2‐terminal extension of high molecular weight bFGF is a nuclear targeting signal (1991)

Basic fibroblast growth factor (bFGF) is a member of the heparin-binding growth factor (HBGF) family that includes at least seven species. These proteins are potent regulators of a number of cellular processes, including cell division and angiogenesis. Multiple forms of bFGF exist differing only in the length of their NH2-terminal extensions. These species of bFGF also have unique subcellular distributions. The smallest form (18 kD) occurs predominantly in the cytosol, while the higher molecular weight forms (22, 22.5, 24 kD) are associated with the nucleus and ribosomes. Here we report that the nuclear localization of the higher molecular weight forms of bFGF derives specifically from the amino acid sequences within the NH2-terminal extension. This has been demonstrated by constructing a chimeric protein containing the NH2-terminal extension of the highest molecular weight form of bFGF fused to beta-galactosidase (beta-gal). After transfection in a transient expression system, the chimeric protein accumulated in the nuclei of transfected cells, while the wild-type beta-gal was found predominantly in the cytoplasm.
Single-feed square-ring microstrip antenna withtruncatedcorners for compact circular polarisation operation (1998)

A novel circular polarisation (CP) design of a single-feed square-ring microstrip antenna with truncated corners is described. Experimental results show that the proposed design has a reduced antenna size as compared to the conventional circularly-polarised square microstrip antenna with truncated corners at a given operating frequency. Also, the required size of the truncated corners for CP operation is larger for the present design than for the conventional design using a square microstrip patch, which suggests that the fabrication tolerance is relaxed for the present design. Typical results are presented and analysed.
Safety and efficacy of sunitinib for advanced non‐clear cell renal cell carcinoma (2015)

Sunitinib was efficacious in the treatment of advanced non-clear cell renal cell carcinoma. Most adverse events were tolerable.
Effect of Cortisone Therapy on Serum Complement Components (1973)

Abstract Male guinea pigs weighing 300 to 400 g were given a daily subcutaneous injection of 5, 20 or 100 mg/kg body weight of cortisone acetate. Measurement of serum cortisol demonstrated sustained blood levels proportional to the dose. All nine components of the classical complement pathway were measured by functional assay. At 5 mg/kg/day significant (p &amp;lt; 0.05) changes noted were a 20 and 51% elevation of the CH50 and C1 titers, respectively. C4, C2, and C3–9 complex were higher than saline treated controls but these increases were not statistically significant. High dose glucocorticoid therapy produced a marked depression in the titer of a number of components. At 20 mg/kg/day C4 and C8 titers were most strongly affected although all components except C1 and C9 were at least 15% lower than controls. At 100 mg/kg the titers of all components except C1 and C9 were markedly depressed. During the first 2 weeks of therapy there was a progressive fall in complement followed by stabilization at these lower levels. On discontinuation of therapy 2 weeks were required before the levels of these components returned to normal. Albumin and globulin levels, hematocrit, spleen size, and percentage of weight gain were unaltered by cortisone therapy. There was no effect on hemolytic complement levels by adding hydrocortisone in vitro to guinea pig serum in comparable doses to those obtained in vivo. Thus, altered catabolic and/or synthetic rates are responsible for the cortisone-induced changes in serum complement.
Intramuscular Delivery of Replicon RNA Encoding ZIKV-117 Human Monoclonal Antibody Protects against Zika Virus Infection (2020)

Monoclonal antibody (mAb) therapeutics are an effective modality for the treatment of infectious, autoimmune, and cancer-related diseases. However, the discovery, development, and manufacturing processes are complex, resource-consuming activities that preclude the rapid deployment of mAbs in outbreaks of emerging infectious diseases. Given recent advances in nucleic acid delivery technology, it is now possible to deliver exogenous mRNA encoding mAbs for <i>in situ</i> expression following intravenous (i.v.) infusion of lipid nanoparticle-encapsulated mRNA. However, the requirement for i.v. administration limits the application to settings where infusion is an option, increasing the cost of treatment. As an alternative strategy, and to enable intramuscular (IM) administration of mRNA-encoded mAbs, we describe a nanostructured lipid carrier for delivery of an alphavirus replicon encoding a previously described highly neutralizing human mAb, ZIKV-117. Using a lethal Zika virus challenge model in mice, our studies show robust protection following alphavirus-driven expression of ZIKV-117 mRNA when given by IM administration as pre-exposure prophylaxis or post-exposure therapy.
Acquired Thermotolerance and Stressed-Phase Growth of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula in Continuous Culture (1997)

The response of an extremely thermoacidophilic archaeon, Metallosphaera sedula (growth temperature range, 50 to 79(deg)C; optimum temperature, 74(deg)C; optimum pH, 2.0), to thermal stress was investigated by using a 10-liter continuous cultivation system. M. sedula, growing at 74(deg)C, pH 2.0, and a dilution rate of 0.04 hr(sup-1), was subjected to both abrupt and gradual temperature shifts in continuous culture to determine the responses of cell density levels and protein synthesis patterns. An abrupt temperature shift from 74 to 79(deg)C resulted in little, if any, changes in cell density and a small increase in total protein per cell. When the culture temperature was shifted further to 80.5(deg)C, cell density dropped to below 5 x 10(sup6) cells/ml from 10(sup8) cells/ml, leading to washout of the culture. Operation at this temperature and slightly higher temperatures, however, could be achieved by exposing the culture to thermal stress more gradually (0.5(deg)C increments). As a result, stable operation could be maintained at temperatures of up to 81(deg)C, and the washout temperature could be increased to 82.5(deg)C. Continuous culture operation at 81(deg)C for 100 h (stressed phase) led to an approximately sevenfold lower steady-state cell density than that observed for operation at or below 79(deg)C. However, sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis (both one and two dimensional) revealed significantly higher levels (sixfold increase) of a 66-kDa stress response protein (MseHSP60), immunologically related to Thermophilic Factor 55 from Sulfolobus shibatae (J. D. Trent, J. Osipiuk, and T. Pinkau, J. Bacteriol. 172:1478-1484, 1990). If the acclimated culture was returned to a lower temperature (i.e., 74(deg)C), the amount of MseHSP60 returned to levels observed prior to thermal acclimation. Furthermore, when the previously acclimated culture (at 81(deg)C) was shifted back from 74 to 81(deg)C, without going through gradual acclimation steps, the result was the immediate onset of washout, suggesting no residual thermotolerance. This study shows that gradual thermal acclimation of M. sedula could only extend the temperature range of stable growth for this organism by 2(deg)C above its maximal growth temperature, albeit at reduced cell densities. Also, this investigation illustrates the utility of continuous culture for characterizing heat shock response and assessing maximum growth temperatures for extremely thermophilic microorganisms.
RGD‐Porphyrin Conjugates: Synthesis and Potential Application in Photodynamic Therapy (2003)

Abstract We report a convenient solid‐phase synthesis and characterisation of a new class of glycosylated porphyrins bearing the RGD tripeptide, designed for photodynamic cancer therapy. The photocytotoxicities of these compounds against the K562 leukemia cell line are also presented and compared to the effect of Photofrin II ® . (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2003)
A survey of smoothing techniques for ME models (2000)

In certain contexts, maximum entropy (ME) modeling can be viewed as maximum likelihood (ML) training for exponential models, and like other ML methods is prone to overfitting of training data. Several smoothing methods for ME models have been proposed to address this problem, but previous results do not make it clear how these smoothing methods compare with smoothing methods for other types of related models. In this work, we survey previous work in ME smoothing and compare the performance of several of these algorithms with conventional techniques for smoothing n-gram language models. Because of the mature body of research in n-gram model smoothing and the close connection between ME and conventional n-gram models, this domain is well-suited to gauge the performance of ME smoothing methods. Over a large number of data sets, we find that fuzzy ME smoothing performs as well as or better than all other algorithms under consideration. We contrast this method with previous n-gram smoothing methods to explain its superior performance.