gebhart/patrepeval huggingface dataset:
Barite Segregation In Inclined Boreholes (1999)

Abstract Settling and sagging of barite in inclined boreholes may lead to safety and operational problems. To study the effect of rheology on settling, a laboratory tool was designed, consisting of two connected tubes, one inclined and one vertical. The hydrostatic pressure was measured at the bottom of each pipe. Stable and unstable muds can clearly be differentiated through their pressure behaviour. Several muds were studied at simulated static and dynamic conditions. The results show that sagging is most severe during laminar flow and also indicate that the rheological parameters may be used for predicting stability problems. Introduction In weighted drilling mud barite tends to segregate slowly. In directional drilling operations the settling process is accelerated. Barite settles in the lower side of the borehole and starts sliding when the borehole has an inclination above 30 °. This phenomenon is known as barite sagging. Sagging can lead to drilling and completion problems; a density variation or non-linear hydrostatic pressure gradients which can lead to pressure control problems, while thick and tight barite beds can lead to high torque and drag, stuck pipes and plugged boreholes, and even lost circulation. The sag problem is related to the so called Boycott effect(1), first described in 1920. Hanson et al.(2) have investigated the phenomenon and found that most of the sagging occurs while the mud is circulating. The same conclusion was reached by Bern et al.,(3) the sagging tendency is highest at low annular velocities. Zamora and Jefferson(4) presented a method for tracking drilling fluid density variations, which helps to detect, but not to predict drilling mud instability. Jamison and Clements(5) developed a test method to characterise settling and sag tendencies in static drilling fluids, however their equipment was not able to distinguish between settling and sliding. They also found that their data, based upon standard API rheological parameters like PV and YP, were unsuitable for prediction of sagging behaviour. It is apparent from previous works that the most difficult part of the problem is the prediction of drilling mud instabilities for both static and flowing drilling fluids. To date there are no API test procedures for sag testing. A new simple laboratory tool was therefore designed for the purpose to study and develop a method to predict sagging. Drilling Mud Separation Settling of Particles In vertical wells the settling of weighting material is generally not a problem due to the long settling distance. In horizontal wells the distance to the lower side of the wall is only about 0.2 m, which leads to rapid generation of solids beds. The settling velocity of a single spherical particle, vs,l, in a fluid is expressed by Stoke's law(6): Equation 1 (available in full paper) A barite particle (ρp = 4,200 kg/m3) with a diameter (dp) of 20 µm in a fluid of density (ρfluid) 1,500 kg/m3 and a viscosity (µ??of 40 cP will settle at a rate of 53 mm/h.
Stability characteristics of freeze-dried human live virus vaccines. (1976)

Freeze-drying (lyophilization) of biologically active materials such as human live virus vaccines is an accepted method of preserving potency. Since freeze-drying does not assure total stability, a formal system of monitoring stability, i.e., potency, has been established. Two experimental desings are utilized: (1) Long term studies to duplicate normal use storage at -20 degrees C and 2-8 degrees C and (2) Accelerated studies which predict long term stability through regression analysis using the Arrhenius equation. Data summarizing stability experience over a long period of study will be presented.
Principles of dynamic nuclear polarisation (1978)

After a general introduction, two sections are devoted to a survey of the theories of spin temperature and of nuclear relaxation in solids. This is followed by a short section on the so-called well-resolved solid effect, and a much more detailed one on DNP by thermal mixing in the non-linear low spin-temperature domain. One then analyses the various methods of measurement of the nuclear polarisation, as well as an indirect method of detecting the electronic resonance based on the existence of large nuclear polarisations. The final section describes briefly several applications of dynamic nuclear polarisation.
Requirement for Xvent-1 and Xvent-2 gene function in dorsoventral patterning of Xenopus mesoderm (1998)

Xvent-1 and Xvent-2 are members of a novel homeobox subfamily that have been implicated in dorsoventral patterning in Xenopus mesoderm and are thought to function in BMP signalling. Here we investigate the requirement for Xvent function by employing two dominant-negative strategies. Loss of Xvent function dorsalizes ventral mesoderm, induces secondary embryonic axes and directly neuralizes ectoderm. We further find that (1) Xvents act as transcriptional repressors, (2) Xvents function in an additive fashion and (3) a surprising number of genes are able to rescue dominant-negative Xvent phenotypes including Bmp-4, Smad-1 and wild-type Xvents and Xhox3, but not Xwnt-8. The results show that Xvent-1 and Xvent-2 are essential for ventral mesoderm formation and for preventing neural differentiation. A model is suggested to explain how Bmp-4 positional information is converted into distinct cellular responses.
Cenicriviroc for the treatment of non-alcoholic steatohepatitis and liver fibrosis (2018)

Nonalcoholic fatty liver disease (NAFLD) has an increasing prevalence worldwide. At present, no specific pharmacotherapy is approved for NAFLD. Simple steatosis and nonalcoholic steatohepatitis (NASH) can progress to liver fibrosis that is associated with mortality in NAFLD. The recruitment of inflammatory monocytes and macrophages via chemokine receptor CCR2 as well as of lymphocytes and hepatic stellate cells via CCR5 promote the progression of NASH to fibrosis. Areas covered: I summarize preclinical and clinical data on the efficacy and safety of the dual CCR2/CCR5 inhibitor cenicriviroc (CVC, also TBR-652 or TAK-652) for the treatment of NASH and fibrosis. In animal models of liver diseases, CVC potently inhibits macrophage accumulation in the liver and ameliorates fibrosis. In a phase 2b clinical trial (CENTAUR) on 289 patients with NASH and fibrosis, CVC consistently demonstrated liver fibrosis improvement after 1 year of therapy and had an excellent safety profile, leading to the implementation of a phase 3 trial (AURORA). Expert opinion: Preclinical and clinical data support the development of CVC as a safe and potent antifibrotic agent. However, open questions around CVC are the durability of antifibrotic responses, divergent effects on NASH versus fibrosis, potential long-term concerns and the expected path to approval.
Multicolor Spectral Karyotyping of Human Chromosomes (1996)

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.
Chemically induced dynamic nuclear polarization studies of yeast tRNAPhe (1984)

Chemically induced dynamic nuclear polarization (CIDNP) has been observed from yeast tRNAPhe following reaction with photoexcited riboflavin. At 20 degrees C, several resonances of tRNA in the native form show polarization; previous work predicts that only guanosine and its derivatives in single-stranded regions are likely to become polarized [ McCord , E.F., Morden , K. M., Pardi , A., Tinoco , I., Jr., & Boxer, S. G. (1984) Biochemistry (preceding paper in this issue)]. The methyl protons of m22G -26 show strong negative spin polarization, indicating that this residue is accessible. The solvent accessibility of this residue has not been previously demonstrated. In addition, two positively polarized aromatic resonances are observed, which are likely due to two or more G(H8) protons, including those of G-20, m22G -26, and/or Gm-34. For temperatures below 50 degrees C, a negatively polarized signal in the aromatic region is shown to arise from cross relaxation with the methyl group protons of m22G -26. This indicates the proximity of an aromatic proton, probably H2 of A-44, to the methyl groups of m22G -26. At higher temperatures, the CIDNP spectra show polarization of several additional G resonances, including those of m2G -10. These changes in the CIDNP spectra reflect melting of the tertiary and secondary structure of the tRNA. This work is the first use of CIDNP to study a large nucleic acid molecule and exemplifies the value of this technique in probing single-stranded and solvent-accessible regions of tRNA.
Low-Frequency Noise Performance of a Bilayer InZnO–InGaZnO Thin-Film Transistor for Analog Device Applications (2010)

In this letter, we present a comparative study of the low-frequency noise behavior of single-layer InGaZnO and bilayer InZnO-InGaZnO thin-film transistors (TFTs). The normalized noise for the bilayer oxide TFT is three times lower than that for the single-layer oxide TFT, mainly due to the higher mobility of the thin interfacial InZnO layer. The carrier number fluctuation is the dominant low-frequency noise mechanism in both devices. The use of a high-mobility bilayer oxide TFT with scaled gate length is still valid for reducing low-frequency noise.
A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo (2005)

The inability of the immune system to recognize and kill malignant plasma cells in patients with multiple myeloma (MM) has been attributed in part to the ineffective activation of natural killer (NK) cells. In order to activate and target NK cells to the malignant cells in MM we designed a novel recombinant bispecific protein (ULBP2-BB4). While ULBP2 binds the activating NK receptor NKG2D, the BB4 moiety binds to CD138, which is overexpressed on a variety of malignancies, including MM. ULBP2-BB4 strongly activated primary NK cells as demonstrated by a significant increase in interferon-gamma (IFN-gamma) secretion. In vitro, ULBP2-BB4 enhanced the NK-mediated lysis of 2 CD138+ human MM cell lines, U-266 and RPMI-8226, and of primary malignant plasma cells in the allogenic and autologous setting. Moreover, in a nude mouse model with subcutaneously growing RPMI-8226 cells, the cotherapy with ULBP-BB4 and human peripheral blood lymphocytes abrogated the tumor growth. These data suggest potential clinical use of this novel construct in patients with MM. The use of recombinant NK receptor ligands that target NK cells to tumor cells might offer new approaches for other malignancies provided a tumor antigen-specific antibody is available.
A mixed frequency-time approach for quasi-periodic steady-state simulation of multi-level modeled circuits (2003)

A new method which extends circuit level mixed frequency-time (MFT) method for quasi-periodic steady-state simulation to multilevel modeled circuits is presented. The extension is made possible by the use of a analog multi-level simulator iMACSIM and the use of Broyden's method to solve the boundary value equations. It is rigorously shown that the new method efficiently allows top-down design and simulation of a class of mixed signal and RF circuits in a unified CAD environment with component models at several levels of abstraction. Other advantages are that it requires significantly less matrix computation and storage than previously used method, and that it can be implemented easily around any transient simulation program. It also supports IEEE VHDL-AMS design methodology.
Practice parameter: Evidence-based guidelines for migraine headache (an evidence-based review) [RETIRED] (2000)

Mission statement
Auto-porting and rapid prototyping with application to wireless and nomadic network algorithms (1996)

Today we are faced with a need to find better ways to design, evaluate, and implement a plethora of wireless and nomadic networking algorithms that are emerging. Simulation is a key tool for evaluation. Unfortunately, development tools used in the past do not integrate or provide a direct path to implementation from simulation. This dissertation identifies a general framework which can be used to implement network algorithms into simulation, implementation testbed, or the Rapid Prototyper for validation and evaluation. As an example, we demonstrated this methodology in the application area of wireless and nomadic network algorithms. Example studies have worked with wireless clustering algorithms and the Nomadic Router to successfully validate the feasibility of using this framework. We found that these tools have reduced programming and development time for porting and evaluating a number of wireless and nomadic network algorithms from a matter of months to a matter of a few hours.
The underlying language support for this integrated environment utilizes Maisie, a parallel discrete-event based simulation language and C. Applications, algorithms, protocols, models, and various libraries are able to execute transparently in simulation and implementation. The Auto-porting interface provides a flexible and customizable integration framework for utilizing socket-based communication and for translating code between Maisie and C. The rapid prototyping interface maps the various modules into the simulation and implementation environments for experimentation, validation, and feedback. The Rapid Prototyper developed provides host system transparency thus providing auto-porting capability to move algorithms from simulation to implementation and the ability to rapidly prototype wireless and nomadic network algorithms independent of the host operating system.
Cell Separation, Centrifugation (2010)

Abstract A review of the different types of centrifuges used in industrial biotechnology is compiled, together with brief explanations of their function. The fluid and particle dynamics in the centrifuges is reviewed with some emphasis on the behavior of cells and other particles, and susceptibility to the shear forces acting on them. The classical mathematical models for the theoretical size, Σvalue, and KQ value are characterized. The concept of separation efficiency, the deviation from ideal capacity, due to the simplicity of the mathematical model for the Σ value is defined. The considerations when selecting type and size of centrifuge for a given process are described, as well as the evaluation and enhancement of separability of the given process stream. The importance of small‐scale lab and pilot tests for a successful scale‐up is emphasized. Applications in harvesting of bacteria, yeasts, and mammalian cells are reviewed, as well as cell debris and inclusion body separations. Extraction—solvent and two‐phase aqueous—application examples are given. Finally, some plant and operation considerations such as plant design, CIP, SIP, containment, asepsis, and sterilization are briefly reviewed.
Layer-by-Layer Assembly of Heterogeneous Modular Nanocomposites (2012)

Layer-by-layer (LbL) assembly of nanoparticles and polyelectrolyte multilayers into alternating nonrepetitive strata (modules) demonstrates an important advance in heterogeneous nanocomposites from random or repetitive distributions of nanoparticles to a versatile modular design. The morphology and composition for each module are determined by the LbL assembly conditions, as confirmed by cross-section transmission electron microscopy (TEM) and by X-ray photoelectron spectroscopy (XPS). Thickness and spacing of the modules are maintained in the 5–50 nm range relevant for nanoscale proximal interactions.
Botulinum Neurotoxin Serotype a Specific Cell-Based Potency Assay to Replace the Mouse Bioassay (2012)

Botulinum neurotoxin serotype A (BoNT/A), a potent therapeutic used to treat various disorders, inhibits vesicular neurotransmitter exocytosis by cleaving SNAP25. Development of cell-based potency assays (CBPAs) to assess the biological function of BoNT/A have been challenging because of its potency. CBPAs can evaluate the key steps of BoNT action: receptor binding, internalization-translocation, and catalytic activity; and therefore could replace the current mouse bioassay. Primary neurons possess appropriate sensitivity to develop potential replacement assays but those potency assays are difficult to perform and validate. This report describes a CBPA utilizing differentiated human neuroblastoma SiMa cells and a sandwich ELISA that measures BoNT/A-dependent intracellular increase of cleaved SNAP25. Assay sensitivity is similar to the mouse bioassay and measures neurotoxin biological activity in bulk drug substance and BOTOX® product (onabotulinumtoxinA). Validation of a version of this CBPA in a Quality Control laboratory has led to FDA, Health Canada, and European Union approval for potency testing of BOTOX®, BOTOX® Cosmetic, and Vistabel®. Moreover, we also developed and optimized a BoNT/A CBPA screening assay that can be used for the discovery of novel BoNT/A inhibitors to treat human disease.
Rapid responses to anakinra in patients with refractory adult‐onset Still's disease (2005)

Refractory AOSD appears to be IL-1-mediated since anakinra decreases hematologic, biochemical, and cytokine markers and also produces rapid reductions in systemic and local inflammation. Reported efficacy of tumor necrosis factor-blocking therapies in AOSD may be due to a reduction in IL-1.
Role of certain virulence factors in a murine model of Staphylococcus aureus arthritis (1997)

The susceptibility of male Swiss white mice (MF1) to Staphylococcus aureus-induced arthritis was investigated with wild-type strain allelic replacement mutants. Comparison was made with a known mouse arthritogenic strain. The development and severity of arthritis were dependent both on the numbers of live bacteria injected intravenously and also on the mutant used; the ID50 ranged from (5 X 10(6))-(1 x 10(8)) cfu. The results indicate that expression of the genes associated with virulence, including those for protein A and alpha-haemolysin, play a major role in the pathogenesis of staphylococcal septic arthritis. When either virulence component was carried by the S. aureus variant, a greater degree of inflammation, pannus formation and cartilage destruction was detected histologically. Loss of one or more virulence factors lowered the septic arthritis severity score based on clinical and histological parameters.
Intrathecal Upregulation of Granulocyte Colony Stimulating Factor and Its Neuroprotective Actions on Motor Neurons in Amyotrophic Lateral Sclerosis (2006)

To investigate cytokine/chemokine changes in amyotrophic lateral sclerosis (ALS), we simultaneously measured 16 cytokine/chemokines (interleukin [IL]-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 [p70], IL-13, IL-17, interferon-gamma, tumor necrosis factor-alpha, granulocyte colony stimulating factor [G-CSF], macrophage chemoattractant protein-1 [MCP-1], and macrophage inflammatory protein-1beta) in cerebrospinal fluid (CSF) and sera from 37 patients with sporadic ALS and 33 controls using a multiplexed fluorescent bead-based immunoassay. We also conducted immunohistochemical analyses from 8 autopsied ALS cases and 6 nonneurologic disease controls as well as cell culture analyses of relevant cytokines and their receptors. We found that concentrations of G-CSF and MCP-1 were significantly increased in ALS CSF compared with controls. In spinal cords, G-CSF was expressed in reactive astrocytes in ALS cases but not controls, whereas G-CSF receptor expression was significantly decreased in motor neurons of spinal cords from ALS cases. Biologically, G-CSF had a protective effect on the NSC34 cell line under conditions of both oxidative and nutritional stress. We suggested that G-CSF has potentially neuroprotective effects on motor neurons in ALS and that downregulation of its receptor might contribute to ALS pathogenesis. On the other hand, MCP-1 correlated with disease severity, which may aggravate motor neuron damage.
Short-Term Freeway Traffic Volume Forecasting Using Radial Basis Function Neural Network (1998)

A radial basis function (RBF) neural network has recently been applied to time-series forecasting. The test results of an RBF neural network in forecasting short-term freeway traffic volumes are provided. Real observations of freeway traffic volumes from the San Antonio TransGuide System have been used in these experiments. For comparison of forecasting performances, Taylor series, exponential smoothing method (ESM), double exponential smoothing method, and backpropagation neural network were also designed and tested. The RBF neural network model provided the best performance and required less computational time than BPN. It seems that RBF and ESM can be a viable forecasting routine for advanced traffic management systems. There are some tradeoffs between RBF and ESM. Although the performance of ESM is inferior to RBF, the former does not need a complicated training process or historic database, and vice versa. However, even in the best performance case, 35 percent of the forecast traffic volumes showed 10 percent or more percentage errors. This means that we cannot heavily depend on the forecast traffic volumes as long as we are utilizing the models tested. Further work is needed to provide a more reliable traffic forecasting model.
Antisense RNA inhibition of phosphoprotein p18 expression abrogates the transformed phenotype of leukemic cells. (1996)

Phosphoprotein p18 was identified originally on the basis of its very high level of expression in leukemic cells of different lineages. Changes in the level of p18 accumulation and phosphorylation associated with induction of differentiation of leukemic cells suggested a potential role for this phosphoprotein in cellular proliferation and differentiation and possibly in malignant transformation. Recent studies have demonstrated that p18 plays an important role in cell cycle progression by serving as a substrate for p34(cdc2) kinase. These studies showed that inhibition of p18 expression in leukemic cells results in growth retardation and accumulation of cells in G(2)-M. In this study, we explore the potential role of p18 in cellular transformation by investigating the effects of inhibition of p18 expression on the malignant phenotype of K562 erythroleukemia cells. These studies show that antisense inhibition of p18 expression in leukemic cells results in growth arrest at a lower saturation density, loss of serum independence, and loss of anchorage-independent growth in vitro. In addition, inhibition of p18 expression results in a marked inhibition of tumorigenicity of leukemic cells in vivo in the severe combined immune deficiency mouse model. These studies demonstrate that the high level of p18 expression in leukemic cells is necessary for the maintenance of the transformed phenotype and suggest p18 as a potential target for antileukemic interventions.
Glycan side chains on naturally presented MHC class II ligands (2005)

The molecular characterization of unknown naturally presented major histocompatibility complex (MHC) class II glycopeptides carrying complex glycans has so far not been achieved, reflecting the different fragmentation characteristics of sugars and peptides in mass spectrometric analysis. Human leukocyte antigen (HLA)-DR-bound peptides were isolated by affinity purification, separated via high performance liquid chromatography and analyzed by matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry. We were able to identify two naturally processed MHC class II ligands, CD53(122-136) and CD53(121-136), carrying complex N-linked glycan side chains by a combination of in-source and collision-induced fragmentation on a quadrupole time-of-flight tandem mass spectrometer.