gebhart/patrepeval huggingface dataset:
Saturation kinetics of the S0 to S2 optical transition in isolated diphenylhexatriene (1985)

A three level kinetic model is fitted to fluorescence intensities measured as a function of excitation intensity for the S0 to S2 transition of isolated diphenylhexatriene. Parameters derived in the fit are coupled with experimental observations to bracket the rate of energy redistribution from S2 to vibrationally excited levels of S1 and S0 to between 1010 and 4×1012 s−1. The rate of excitation, which is directly proportional to oscillator strength, is precisely determined in the fit to be 3.7×108 s−1 per μJ at the peak of our 6 ns FWHM laser pulse.
SIMULTANEOUS PRODUCTION OF HIGH-PURITY HYDROGEN AND SEQUESTRATION-READY CO2 FROM SYNGAS (2003)

This final report summarizes the progress made on the program ''Simultaneous Production of High-Purity Hydrogen and Sequestration-Ready CO{sub 2} from Syngas (contract number DE-FG26-99FT40682)'', during October 2000 through September of 2003. GE Energy and Environmental Research (GE-EER) and Southern Illinois University (SIU) at Carbondale conducted the research work for this program. This program addresses improved methods to efficiently produce simultaneous streams of high-purity hydrogen and separated carbon dioxide from synthesis gas (syngas). The syngas may be produced through either gasification of coal or reforming of natural gas. The process of production of H{sub 2} and separated CO{sub 2} utilizes a dual-bed reactor and regenerator system. The reactor produces hydrogen and the regenerator produces separated CO{sub 2}. The dual-bed system can be operated under either a circulating fluidized-bed configuration or a cyclic fixed-bed configuration. Both configurations were evaluated in this project. The experimental effort was divided into lab-scale work at SIU and bench-scale work at GE-EER. Tests in a lab-scale fluidized bed system demonstrated the process for the conversion of syngas to high purity H{sub 2} and separated CO{sub 2}. The lab-scale system generated up to 95% H{sub 2} (on a dry basis). Extensive thermodynamic analysis of chemical reactions between the syngas and the fluidized solids determined an optimum range of temperature and pressure operation, where the extent of the undesirable reactions is minimum. The cycling of the process between hydrogen generation and oxygen regeneration has been demonstrated. The fluidized solids did not regenerate completely and the hydrogen purity in the reuse cycle dropped to 70% from 95% (on a dry basis). Changes in morphology and particle size may be the most dominant factor affecting the efficiency of the repeated cycling between hydrogen production and oxygen regeneration. The concept of simultaneous production of hydrogen and separated stream of CO{sub 2} was proved using a fixed bed 2 reactor system at GE-EER. This bench-scale cyclic fixed-bed reactor system designed to reform natural gas to syngas has been fabricated in another coordinated DOE project. This system was modified to reform natural gas to syngas and then convert syngas to H{sub 2} and separated CO{sub 2}. The system produced 85% hydrogen (dry basis).
Increased Expression of Androgen Receptor Coregulator MAGE-11 in Prostate Cancer by DNA Hypomethylation and Cyclic AMP (2009)

Melanoma antigen gene protein-A11 (MAGE-11) of the MAGE family of cancer germ-line antigens increases androgen receptor (AR) transcriptional activity through its interaction with the AR NH(2)-terminal FXXLF motif. The present study investigated the regulatory mechanisms that control MAGE-11 expression during androgen deprivation therapy and prostate cancer progression. Studies include the CWR22 xenograft model of human prostate cancer, clinical specimens of benign and malignant prostate, and prostate cancer cell lines. MAGE-11 mRNA levels increased 100- to 1,500-fold during androgen deprivation therapy and prostate cancer progression, with highest levels in the castration-recurrent CWR22 xenograft and clinical specimens of castration-recurrent prostate cancer. Pyrosequencing of genomic DNA from prostate cancer specimens and cell lines indicated the increase in MAGE-11 resulted from DNA hypomethylation of a CpG island in the 5' promoter of the MAGE-11 gene. Sodium bisulfite sequencing of genomic DNA from benign and malignant prostate tumors and prostate cancer cell lines revealed DNA hypomethylation at individual CpG sites at the transcription start site were most critical for MAGE-11 expression. Cyclic AMP (cAMP) also increased MAGE-11 expression and AR transcriptional activity in prostate cancer cell lines. However, cAMP did not alter DNA methylation of the promoter and its effects were inhibited by extensive DNA methylation in the MAGE-11 promoter region. Increased expression of the AR coregulator MAGE-11 through promoter DNA hypomethylation and cAMP provides a novel mechanism for increased AR signaling in castration-recurrent prostate cancer.
Throw and Tilt – Seamless Interaction across Devices Using Mobile Phone Gestures (2008)

Abstract: To overcome limitations of small screens and to provide intuitive ways of interacting with personal data, this work addresses the seamless combination of sensor-enabled phones with large displays. An intuitive basic set of tilt gestures is introduced for a stepwise or continuous interaction with both mobile applications and distant user interfaces by utilizing the handheld as a remote control. In addi-tion, we introduce throwing gestures to transfer media documents to a large dis-play. By means of these gestures, we also propose transferring a running interface from a mobile phone to a large screen (to improve usability) and back (to achieve mobility). We demonstrate the feasibility of the interaction methods with several application prototypes facilitating a very natural flow of interaction. 1
A Single-Blinded, Randomized Pilot Study Evaluating Effects of Electroacupuncture in Diabetic Patients with Symptoms Suggestive of Gastroparesis (2008)

This study demonstrates that short-term EA at the Zusanli and Hegu points effectively reduces the dyspeptic symptoms of diabetic gastroparesis and accelerates solid gastric emptying. Sustained improvement in dyspeptic symptoms was observed at 2 weeks after the end of the trial. Its potential for treating gastroparesis may be explored, and a larger trial is required to draw definitive conclusions.
The human cytomegalovirus UL37 immediate-early regulatory protein is an integral membrane N-glycoprotein which traffics through the endoplasmic reticulum and Golgi apparatus (1996)

The human cytomegalovirus (HCMV) UL37 immediate-early gene is predicted to encode a type I membrane-bound glycoprotein, gpUL37. Following expression of the UL37 open reading frame in vitro, its signals for translocation and N-glycosylation were recognized by microsomal enzymes. Its orientation in the microsomes is that of a type I protein. gpUL37 produced in HCMV-infected human cells was selectively immunoprecipitated by rabbit polyvalent antiserum generated against the predicted unique domains of the UL37 open reading frame and migrated as an 83- to 85-kDa protein. Tunicamycin treatment, which inhibits N-glycosylation, increased the rate of migration of the UL37 protein to 68 kDa, verifying its modification by N-glycosylation in HCMV-infected cells. Consistent with this observation, gpUL37 was found to be resistant to digestion with either endoglycosidase F or H but sensitive to peptide N-glycosidase F digestion. These results suggested that gpUL37 is N-glycosylated and processed in both the endoplasmic reticulum (ER) and the Golgi apparatus. Direct demonstration of passage of gpUL37 through the ER and the Golgi was obtained by confocal microscopy. gpUL37 colocalized with protein disulfide isomerase, a protein resident in the ER, and with a Golgi protein. Subcellular fractionation of HCMV-infected cells demonstrated that gpUL37 is an integral membrane protein. Taken together, our results demonstrate that the HCMV gpUL37 immediate-early regulatory protein is a type I integral membrane N-glycoprotein which traffics through the ER and the Golgi network.
Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs (2007)

Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes ( Chat , Th , Slc6a4 , Slc6a2 , Etv1 , Ntsr1 , Drd2 , Drd1 , Pcp2 , and Cmtm5 ) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the &gt;500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.
When Cells Become Organelle Donors (2013)

More than 40 variations of intercellular organelle transfer, such as a mitochondria or lysosome originating in one cell being transported to another cell, have been described. We review the mechanisms and subcellular structures by which, and conditions under which, transfer occurs, with particular attention paid to the role of external cell stress in activating transfer. We propose a research agenda for answering key questions in this burgeoning field.
The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functions (2007)

The human heat shock protein 70 (Hsp70) family contains at least eight homologous chaperone proteins. Endoplasmatic reticulum and mitochondria have their specific Hsp70 proteins, whereas the remaining six family members reside mainly in the cytosol and nucleus. The requirement for multiple highly homologous although different Hsp70 proteins is still far from clear, but their individual and tissue-specific expression suggests that they are assigned distinct biological tasks. This concept is supported by the fact that mice knockout for different Hsp70 genes display remarkably discrete phenotypes. Moreover, emerging data suggest that individual Hsp70 proteins can bring about non-overlapping and chaperone-independent functions essential for growth and survival of cancer cells. This review summarizes our present knowledge of the individual members of human Hsp70 family and elaborate on the functional differences between the cytosolic/nuclear representatives.
Energy-based renal tumor ablation: a review. (2001)

The trend toward minimally invasive options in the management of renal tumors has prompted interest in energy-based ablation techniques as a possible alternative to radical or partial nephrectomy in select patients. Such techniques include radiofrequency thermal ablation, microwave thermotherapy, laser interstitial thermal therapy, high intensity focused ultrasound, interstitial photon radiation ablation, and cryoablation. This review describes the current status of these techniques as they apply to the management of renal tumors.
Biologics through Chemistry: Total Synthesis of a Proposed Dual-Acting Vaccine Targeting Ovarian Cancer by Orchestration of Oligosaccharide and Polypeptide Domains (2009)

Carbohydrate and peptide-based antitumor vaccine constructs featuring clusters of both tumor associated carbohydrate antigens and mucin-like peptide epitopes have been designed, synthesized, and studied. The mucin-based epitopes are included to act, potentially, as T-cell epitopes in order to provoke a strong immune response. Hopefully the vaccine will simulate cell surface architecture, thereby provoking levels of immunity against cancer cell types displaying such characteristics. With this central idea in mind, we designed a new vaccine type against ovarian cancer. Following advances in glycohistology, our design is based on clusters of Gb(3) antigen and also incorporates a MUC5AC peptide epitope. The vaccine is among the most complex targeted constructs to be assembled by chemical synthesis to date. The strategy for the synthesis employed a Gb(3)-MUC5AC thioester cassette as a key building block. Syntheses of both nonconjugate and KLH-conjugated vaccines constructs have been accomplished.
SNAP-25 and synaptotagmin involvement in the final Ca(2+)-dependent triggering of neurotransmitter exocytosis. (1996)

In neurons, depolarization induces Ca2+ influx leading to fusion of synaptic vesicles docked at the active zone for neurotransmitter release. While a number of proteins have now been identified and postulated to participate in the assembly and subsequent disengagement of a vesicle docking complex for fusion, the mechanism that ultimately triggers neuroexocytosis remains elusive. Using a cell-free, lysed synaptosomal membrane preparation, we show that Ca2+ alone is sufficient to trigger secretion of glutamate and furthermore that Ca(2+)-signaled exocytosis is effectively blocked by antibodies and peptides to SNAP-25, a key constituent of the vesicle docking complex. In addition, Ca2+ inhibits the ability of synaptotagmin, a synaptic vesicle protein proposed as a calcium sensor and triggering device, to associate with this docking complex. These results support a model in which Ca(2+)-dependent triggering of neurotransmission at central synapses acts after ATP-dependent potentiation of the docking-fusion complex for membrane fusion.
Linearly fused vs bridged regioselection in the intramolecular 1,3-diyl trapping reaction (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLinearly fused vs bridged regioselection in the intramolecular 1,3-diyl trapping reactionMohammad R. Masjedizadeh, Ingeborg Dannecker-Doerig, and R. Daniel LittleCite this: J. Org. Chem. 1990, 55, 9, 2742–2752Publication Date (Print):April 1, 1990Publication History Published online1 May 2002Published inissue 1 April 1990https://pubs.acs.org/doi/10.1021/jo00296a035https://doi.org/10.1021/jo00296a035research-articleACS PublicationsRequest reuse permissionsArticle Views1104Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Learning Mid-level Filters for Person Re-identification (2014)

In this paper, we propose a novel approach of learning mid-level filters from automatically discovered patch clusters for person re-identification. It is well motivated by our study on what are good filters for person re-identification. Our mid-level filters are discriminatively learned for identifying specific visual patterns and distinguishing persons, and have good cross-view invariance. First, local patches are qualitatively measured and classified with their discriminative power. Discriminative and representative patches are collected for filter learning. Second, patch clusters with coherent appearance are obtained by pruning hierarchical clustering trees, and a simple but effective cross-view training strategy is proposed to learn filters that are view-invariant and discriminative. Third, filter responses are integrated with patch matching scores in RankSVM training. The effectiveness of our approach is validated on the VIPeR dataset and the CUHK01 dataset. The learned mid-level features are complementary to existing handcrafted low-level features, and improve the best Rank-1 matching rate on the VIPeR dataset by 14%.
Cloning, expression and biological activity of a new variant of human interferon alpha identified in virus induced lymphoblastoid cells. (1985)

A synthetic oligonucleotide complementary to a highly conserved sequence in the IFN-alpha gene family, was used to screen a Namalva cDNA library. Among the cDNA clones having typical IFN-alpha traits, one was distinct from previously characterized IFN-alpha cDNAs. E. coli cells carrying this recombinant cDNA plasmid express an alpha-interferon activity. The sequence of this IFN-cDNA is extremely homologous (99.5%) to that of the IFN-alpha J gene and is designated IFN-alpha J1. Several E. coli trp expression plasmids were constructed for efficient transcription and translation of the mature IFN-alpha J1. The maximal level of expression (5 X 10(3) molecules/cells) was obtained from plasmid pJ1-4. A synthetic consensus translation initiation sequence coupled to the trp p/o region (in pJ1-5) proved to be 10 times less effective in promoting metIFN production in bacteria, than the in-vitro mutated trpL initiation sequence carried on pJ1-4. The bacterial IFN-alpha J1 was purified (to over 90% purity) to a specific activity of 1.3 X 10(8) units/mg. The antiviral activity of the purified IFN-alpha J1 was compared with other highly purified IFN-alpha species (bacterial IFN alpha A and alpha C, leukocyte IFN-alpha 1, leukocyte IFN mixture and Namalva IFN preparation) on a large panel of mammalian cell cultures. IFN-alpha J1 exhibits a distinct antiviral activity.
Antibiotic Therapy for Chronic Infection of Pseudomonas in the Lung (1993)

Chronic Pseudomonas aeruginosa lung infection is an endobronchiolitis characterized by the microcolony mode of growth (biofilm). Early colonization is treated with oral ciprofloxacin combinedw ith colistin inhalation. Chronic infection is treated with “maintenance chemotherapy” consisting of β-lactam antibiotics and tobramycin intravenously in combination with colistin inhalation four times yearly.
457. Furano-compounds. Part VII. A synthesis of 2 : 3-dihydropsoralene (1948)

R. T. Foster, A. Robertson and A. Bushra, J. Chem. Soc., 1948, 2254 DOI: 10.1039/JR9480002254
Chromophore-assisted laser inactivation of proteins is mediated by the photogeneration of free radicals. (1994)

Chromophore-assisted laser inactivation (CALI) is a technique that selectively inactivates proteins of interest to elucidate their in vivo functions. This method has application to a wide array of biological questions and an understanding of its mechanism is required for its judicious application. We report here that CALI is not mediated by photoinduced thermal denaturation but by photogenerated free radicals. Thermal diffusion calculations suggest that the temperature changes resulting from CALI are too small to cause thermal denaturation, and Arrhenius plots of CALI are inconsistent with a photothermal mechanism. CALI shows an energy dose reciprocity above a threshold and can be inhibited by free-radical quenchers, thus demonstrating a photochemical mechanism of protein inactivation. The type of quenchers that are effective in inhibiting CALI indicates that the active species is a hydrogen abstractor which is not derived from molecular oxygen. We suggest that the active free-radical species is the hydroxyl radical and its very short lifetime explains the spatial specificity of CALI such that half-maximal damage is effected within 15 A from the dye moiety and no significant damage occurs at 34 A. The data are consistent with free-radical formation resulting from a sequential two-photon process.
Effect of Infectious Bursal Disease Virus Infection on Incidence of Anemia by Chicken Anemia Agent (1980)

N. Yuasa, T. Taniguchi, T. Noguchi, I. Yoshida, Effect of Infectious Bursal Disease Virus Infection on Incidence of Anemia by Chicken Anemia Agent, Avian Diseases, Vol. 24, No. 1 (Jan. - Mar., 1980), pp. 202-209
[Significance of radiation-induced bystander effects in radiation therapy]. (2014)

Since 1994, a Phase I/II clinical study and radiotherapy have carried out using carbon-ion beams produced with the Heavy Ion Medical Accelerator in Chiba (HIMAC) at National Institute of Radiological Sciences. Now we constructed the new treatment facility for the advanced carbon-ion therapy at HIMAC applying a 3D fast spot scanning system with pencil beams. In the field of fundamental biological studies for high-LET heavy ions, there are some reports regarding bystander effects after exposure to alpha particles derived from 238Pu or He-ion microbeams. However, only limited sets of studies have examined bystander effects after exposure to different ion species heavier than helium, such as carbon ions. We have been investigating bystander cellular responses in both normal human and human tumor cells irradiated with the HIMAC carbon ions. Bystander cell-killing effect was observed in the cells harboring wild-type P53 gene, but not in the P53-mutated cells. Moreover, observed bystander effect was suppressed by treating with a specific inhibitor of gap-junction mediated cell-cell communication. There is clear evidence that the carbon-ion irradiation enables the enhanced cell killing in cells with wild-type P53 gene via gap-junction mediated bystander effect.
Hyponatremic Fits in Oxytocin‐Augmented Labors (1979)

ABSTRACT Case reports of three patients who had fits as a consequence of hyponatremia are presented. Each patient was in spontaneous labor at term and received conventional doses of oxytocin for augmentation. The pathogenesis, diagnosis and treatment of this entirely iatrogenic condition are discussed. The differences between these and similar cases in the literature are stressed.
Sensitive method for analysis of strontium in human and animal plasma by graphite furnace atomic absorption spectrophotometry (1995)

For long-term pharmacokinetic studies in humans as well as in experimental animals, an analysis of strontium (Sr) with a low detection limit and high sensitivity is necessary. The data presented here describe the optimization of the furnace program and sample handling for measuring Sr in plasma by using graphite furnace atomic absorption spectrophotometry. The method was validated and applied to studies on the pharmacokinetics of SrCl2 in humans and rats. Calibration curves were linear up to 57.1 nmol/L. The limit of detection and lower limit of quantification were 0.21 and 0.57 nmol/L, respectively. Reciprocal sensitivity was 0.53 nmol/L Sr at A = 0.0044. The intraassay precision was 2.2%, 1.5%, and 1.1% (n = 6) at 4.57, 22.7, and 49.2 nmol/L Sr, respectively. At these concentrations, the interassay precision and recovery were 0.7%, 1.5%, and 1.8% and 100.4%, 99.1%, and 100.6% (n = 12), respectively. The endogenous Sr concentration in human plasma samples was 0.27 +/- 0.07 mumol/L (n = 18). Pharmacokinetic studies in a human volunteer and in rats demonstrated that this procedure was suited for measuring low Sr concentrations and was applicable to small sample volumes.