gebhart/patrepeval huggingface dataset:
Early activation of transcription factor NF-κB during ischemia in perfused rat heart (1999)

The transcription factor nuclear factor kappaB (NF-kappaB) regulates multiple immediate-early gene expressions involved in immune and inflammatory responses and cellular defenses. Ischemia-reperfusion induces many immediate-early gene expressions, but little is known about the NF-kappaB activation in myocardium during ischemia and reperfusion. This study demonstrated that ischemia alone rapidly induced NF-kappaB activation in the myocardium of isolated working rat hearts. Electrophoretic mobility shift assay showed that NF-kappaB binding activity significantly increased in the nucleus after 5 min of ischemia and remained elevated for up to 30 min. Western blot analysis suggested that the levels of inhibitory IkappaBalpha protein in the cytoplasm became markedly decreased at 4, 5, 7.5, and 10 min of ischemia but were gradually restored following 10 min of ischemia. Reduction of IkappaBalpha protein in the cytoplasm by ischemia resulted in NF-kappaB translocation to the nucleus. Northern blot hybridization showed that IkappaBalpha mRNA levels were not significantly elevated during myocardial ischemia. Pyrrolidine dithiocarbamate, an antioxidant, significantly inhibited the loss of IkappaBalpha protein from the cytoplasm and prevented NF-kappaB binding activity in the nucleus. Reperfusion following short periods of ischemia augmented NF-kappaB binding activity in the nucleus induced by ischemia. The results suggest that early activation of NF-kappaB induced by ischemia in the myocardium could be a signal mechanism for controlling and regulating immediate-early gene expression during ischemia-reperfusion.
Apoptosis as a mechanism of cell death in cultured T lymphoblasts acutely infected with HIV-1. (1991)

The mechanisms by which HIV-1 infection kills T lymphocytes are not clearly established. Apoptosis is an internally programmed cell death pathway that may regulate both T cell development and senescence, and that is characterized by cleavage of DNA at internucleosomal regions. The present experiments show that acute HIV-1 infection of MT2 lymphoblasts and activated normal peripheral blood mononuclear cells induces apoptosis. The addition of anti-gp120 neutralizing antibody, after HIV-1 infection of MT2 cells, permitted sustained high levels of viral replication, but blocked apoptosis and cell death. Apoptosis may account for the direct cytopathologic effects of HIV-1 in T cells.
Iron oxide–gold core–shell nanoparticles and thin film assembly (2005)

This paper reports findings of an investigation of the synthesis of monolayer-capped iron oxide and core (iron oxide)–shell (gold) nanocomposite and their assembly towards thin film materials. Pre-synthesized and size-defined iron oxide nanoparticles were used as seeding materials for the reduction of gold precursors, which was shown to be effective for coating the iron oxide cores with gold shells (Fe oxide@Au). The unique aspect of our synthesis is the formation of Fe oxide@Au core–shell nanoparticles with controllable surface properties. The novelty of our assembly strategy is the exploitation of the ligand-exchange reactivity at the gold shells for the thin film assembly of the core–shell nanoparticles. The core–shell nanocomposites and assemblies have been characterized using TEM, XRD, XPS, FTIR, TGA, and DCP-AES techniques. In addition to evidence from TEM detection of the change in particle size, UV-Vis observation of the change in the surface plasmon resonance band, and XRD detection of disappearance of the magnetite diffraction peaks after coating the gold shell, the formation of the core–shell morphology was further confirmed by DCP-AES composition analysis of Au and Fe in the molecularly-mediated thin film assembly of Fe oxide@Au particles. The interparticle ligand exchange–precipitation chemistry at the gold shell is to our knowledge the first example demonstrating the inter-shell reactivity for constructing thin films of Fe oxide@Au particles. The results have provided important insights into the design of interfacial reactivities via core–shell nanocomposites for magnetic, catalytic and biological applications.
Detection and characterization of fungal infections of Ammophila arenaria (marram grass) roots by denaturing gradient gel electrophoresis of specifically amplified 18s rDNA (1997)

Marram grass (Ammophila arenaria L.), a sand-stabilizing plant species in coastal dune areas, is affected by a specific pathosystem thought to include both plant-pathogenic fungi and nematodes. To study the fungal component of this pathosystem, we developed a method for the cultivation-independent detection and characterization of fungi infecting plant roots based on denaturing gradient gel electrophoresis (DGGE) of specifically amplified DNA fragments coding for 18S rRNA (rDNA). A nested PCR strategy was employed to amplify a 569-bp region of the 18S rRNA gene, with the addition of a 36-bp GC clamp, from fungal isolates, from roots of test plants infected in the laboratory, and from field samples of marram grass roots from both healthy and degenerating stands from coastal dunes in The Netherlands. PCR products from fungal isolates were subjected to DGGE to examine the variation seen both between different fungal taxa and within a single species. DGGE of the 18S rDNA fragments could resolve species differences from fungi used in this study yet was unable to discriminate between strains of a single species. The 18S rRNA genes from 20 isolates of fungal species previously recovered from A. arenaria roots were cloned and partially sequenced to aid in the interpretation of DGGE data. DGGE patterns recovered from laboratory plants showed that this technique could reliably identify known plant-infecting fungi. Amplification products from field A. arenaria roots also were analyzed by DGGE, and the major bands were excised, reamplified, sequenced, and subjected to phylogenetic analysis. Some recovered 18S rDNA sequences allowed for phylogenetic placement to the genus level, whereas other sequences were not closely related to known fungal 18S rDNA sequences. The molecular data presented here reveal fungal diversity not detected in previous culture-based surveys.
Analysis on Quench Propagation Characteristics of HTS Tape (2000)

The main issues for the power application of High Tc superconducting tape are lower AC loss and higher stability conditions. HTS tape has large stability margin by high heat capacity of superconductor itself and high temperature margin. But, it can be damaged by continuous heat generation at quench point, because normal zone propagation velocity by generating heat is very low. Here, we analyze the quench propagation characteristics using finite element method for BSCCO-2223 HTS tape.
Simultaneous degradation of organophosphorus pesticides and p‐nitrophenol by a genetically engineered Moraxella sp. with surface‐expressed organophosphorus hydrolase (2001)

Moraxella sp., a native soil organism that grows on p-nitrophenol (PNP), was genetically engineered for the simultaneous degradation of organophosphorus (OP) pesticides and p-nitrophenol (PNP). The truncated ice nucleation protein (INPNC) anchor was used to target the pesticide-hydrolyzing enzyme, organophosphorus hydrolase (OPH), onto the surface of Moraxella sp., alleviating the potential substrate uptake limitation. A shuttle vector, pPNCO33, coding for INPNC-OPH was constructed and the translocation, surface display, and functionality of OPH were demonstrated in both E. coli and Moraxella sp. However, whole cell activity was 70-fold higher in Moraxella sp. than E. coli. The resulting Moraxella sp. degraded organophosphates as well as PNP rapidly, all within 10 h. The initial hydrolysis rate was 0.6 micromol/h/mg dry weight, 1.5 micromol/h/mg dry weight, and 9.0 micromol/h/mg dry weight for methyl parathion, parathion, and paraoxon, respectively. The possibility of rapidly degrading OP pesticides and their byproducts should open up new opportunities for improved remediation of OP nerve agents in the future.
RING DISCHARGE EXCITATION OF GAS ION LASERS (1965)

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Study of corneal ablation with picosecond laser pulses at 211 nm and 263 nm (1996)

Background and Objective Corneal ablation has been studied by picosecond laser pulses in the far-UV region. Study Design/Materials and Methods Laser pulses of 25 ps duration at 211 nm and 263 nm wavelengths and a 1 kHz repetition rate have been used to ablate human and rabbit corneas. The dependence of the etch rate on laser fluence has been measured at both wavelengths. The collateral tissue damage has been investigated by electron microscopy. Results The ablation threshold for human cornea is determined to be about 3.0 mJ/cm2 at 211 nm, while the thresholds for rabbit cornea are about 2.3 mJ/cm2 at 211 nm and 8.0 mJ/cm2 at 263 nm. The slopes of the ablation curves and the dimensions of the damage zones have also been determined. Conclusion We compare these results to the existing data on corneal ablation by nanosecond UV pulses and discuss the deficiency of the photochemical model. Experimental results are analyzed in terms of a model that features plasma ablation assisted by chromophore absorption. © 1996 Wiley-Liss, Inc.
Human Immunodeficiency Virus Type 1 Infection in Homosexual Men Who Remain Seronegative for Prolonged Periods (1989)

Infection with the human immunodeficiency virus type 1 (HIV-1), as demonstrated by viral cultures, has been described in some patients before antibodies to HIV-1 can be detected, but the duration and frequency of such latent infections are uncertain. We selected prospectively a cohort of 133 seronegative homosexual men who continued to be involved in high-risk sexual activity, and we cultured 225 samples of their peripheral-blood lymphocytes, using mitogen stimulation to activate the integrated HIV-1 genome. HIV-1 was isolated in blood samples from 31 of the 133 men (23 percent), 27 of whom have remained seronegative for up to 36 months after the positive culture. The other four men seroconverted 11 to 17 months after the isolation of HIV-1. In three of them, we studied cryopreserved lymphocytes obtained earlier, using the polymerase chain reaction to amplify small amounts of viral DNA, and we demonstrated that HIV-1 provirus had been present 23, 35, and 35 months before seroconversion. We conclude that HIV-1 infection in homosexual men at high risk may occur at least 35 months before antibodies to HIV-1 can be detected. A prolonged period of latency in such infections may be more common than previously recognized; the degree of infectiousness during such periods is unknown.
Origin of magnetic field enhancement in the generation of terahertz radiation from semiconductor surfaces (2001)

We present a theory of the magnetic field enhancement of terahertz (THz) emission from photogenerated carriers in the surface depletion region of a semiconductor. A combination of the Drude-Lorentz model for the carrier dynamics with an appropriate solution of the radiation problem is sufficient to explain the strong B -field enhancement in THz radiation that has been observed experimentally. The effect arises primarily from the increased radiation efficiency of transient currents flowing in the plane of the surface. The model provides quantitative agreement with experiment for the pronounced angular dependence of the enhancement and predicts the correct trend for the enhancement in a variety of materials.
His Electrogram Alternans Reveal Dual-Wavefront Inputs Into and Longitudinal Dissociation Within the Bundle of His (2001)

Background His electrogram (HE) amplitude and morphology changes were observed in our previous studies during transition from “fast” to “slow” atrioventricular nodal (AVN) conduction. This phenomenon and its significance for the dual-AVN electrophysiology are not well recognized and have not been studied. Methods and Results Experiments were performed on 17 healthy rabbit atrial-AVN preparations during standard programmed electrical pacing. HEs were mapped along the His bundle with roving surface electrodes, along with recording of cellular action potentials (APs). HEs recorded from the superior margin of the His bundle were of greater amplitude during basic beats and decreased substantially, by 42±19% ( P &lt;0.01), when premature A 1 A 2 shortened to 178±20 ms. In contrast, the HEs from the inferior margin increased dramatically, 2.9±1.7 times ( P &lt;0.01), during short A 1 A 2 and remained high until AVN block occurred. In addition, during long A 1 A 2 , the superior HEs consistently preceded the inferior by 1.9±0.7 ms. In contrast, at short A 1 A 2 , the superior HEs occurred 2.7±0.8 ms after the inferior. Cellular AP recordings demonstrated clearly the presence of and the transition between early (fast) and late (slow) excitation wavefronts that accompanied HE alternans. Conclusions The morphological-electrophysiological evidence from the AV junction suggests that fast and slow wavefronts reach the His bundle differently, producing functional longitudinal dissociation into 2 domains. The characteristic HE alternans recorded from these domains are a new sensitive tool to determine the presence of distinctly different wavefronts and their participation in the conduction during reentrant or other arrhythmias. These findings provide further understanding of the mechanisms of dual-AVN electrophysiology.
8-PSK trellis codes for a Rayleigh channel (1992)

A suboptimal trellis coding approach based on the concept of combining a good convolutional code and bit interleavers is presented. The aim is to improve the reliability of digital radio communication over a fading channel. It is shown that over a Rayleigh channel and for a fixed code complexity the proposed system is superior to the baseline system. Its performance is analyzed using the generalized R/sub o/ and the upper bound on the bit error rate. The results suggest that on a Rayleigh channel, the standard trellis codes may not be the correct approach for improving the reliability of the communication channel. The discussion is restricted to a rate 2/3 coded system with 8-PSK modulation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Globalism, nationalism and selective importation (2003)

Understanding the perceptions of the Chinese decision-makers is paramount if we are to understand the decision to import Western state products and to integrate the country into the world system. This chapter tries to answer the question: Why does the Chinese leadership want to import Western state products selectively? I will examine how China's leaders formed their globalist worldview over the course of the country's development, and then look at why the post-Mao leadership wants to select economic rather than political products of Western states in its efforts to transform the state.
THE INTEGRATION OF NAVIGATIONAL INFORMATION--THE ELECTRONIC CHART (1989)

This article describes the two existing major types of electronic charts and their capabilities. Consideration is also given to complementary publications such as tide tables, sailing directions and light lists and to how their information is used on the electronic chart. The legal aspects of electronic chart use are discussed in relation to the Safety of Life at Sea (SOLAS) Convention requirements for the carriage by vessels of up-to-date nautical publications necessary for an intended voyage. The future potential of the electronic chart is assessed.
Hepatocyte growth factor induces Wnt-independent nuclear translocation of beta-catenin after Met-beta-catenin dissociation in hepatocytes. (2002)

Hepatocyte growth factor (HGF) and Wnt signaling pathways have been shown to be important in embryogenesis and carcinogenesis. The aim of this study was to elucidate the mechanism of functional similarities observed in the two pathways. We used normal rat liver, primary hepatocyte cultures and a dominant-negative Met expression system to study the effect of HGF on Wnt pathway components. We demonstrate novel association of beta-catenin and Met, a tyrosine kinase receptor of HGF, at the inner surface of the hepatocyte membrane. HGF induces dose-dependent nuclear translocation of beta-catenin in primary hepatocyte cultures that is Wnt independent. The source of beta-catenin for translocation in hepatocytes is the Met-beta-catenin complex, which appears to be independent of the E-cadherin-beta-catenin complex. To test the functionality of this association, we used a dominant-negative Met expression system that expresses only the extracellular and transmembrane regions of the beta-subunit of Met. A loss of Met-beta-catenin association resulted in abrogation of nuclear translocation of beta-catenin upon HGF stimulation. This event is tyrosine phosphorylation dependent, and the association of Met and beta-catenin is crucial for this event. We conclude that the HGF causes similar redistribution of beta-catenin as Wnt-1 in the hepatocytes and that this effect is attributable to subcellular association of Met and beta-catenin. The intracellular kinase domain of Met is essential for tyrosine phosphorylation and nuclear translocation of beta-catenin. Part of the multifunctionality of HGF might be attributable to nuclear beta-catenin and the resulting target gene expression.
Antibody-Based Delivery of IL2 and Cytotoxics Eradicates Tumors in Immunocompetent Mice (2014)

Antibody-drug conjugates are increasingly being used for cancer therapy, but little is known about their ability to promote anticancer immunity, which may lead to long-lasting remissions. We investigated the therapeutic effect of antibody-based pharmacodelivery of cemadotin, a cytotoxic drug, and IL2, a strong proinflammatory cytokine. Using the F8 antibody, which selectively localizes to the tumor neovasculature, combination treatment led to tumor eradication, in a process dependent on CD8(+) T cells and natural killer cells in the C1498 syngeneic mouse model of acute myelogenous leukemia. The clinical combination of antibody-drug conjugates and antibody-cytokine proteins should be facilitated by their orthogonal toxicity profiles.
Targeting of Bone-Derived Insulin-Like Growth Factor-II by a Human Neutralizing Antibody Suppresses the Growth of Prostate Cancer Cells in a Human Bone Environment (2009)

Our results indicate that IGF-II plays an important role in the prostate cancer cell growth in human bone, suggesting that targeting it by neutralizing antibodies offers a new therapeutic strategy for bone metastasis from prostate cancer.
Differences in the Binding to Fibrin of Urokinase and Tissue Plasminogen Activator (1972)

It has been reported by many investigators that platelets adhere to collagen fibers (9) and by us that platelets adhere to injured endothelial cells in vivo in small mesenteric arterioles in guinea pigs (2). This study shows platelets aggregating to form thrombi on the amorphous cytoplasm of endothelium where the ATP-ase activity has been located and where some unrecognizable part of the basement membrane may exist. It is postulated that injury to the endothelial cell exposes these ATP-ases which release the adenosine diphosphate observed in the blood in the damaged vessel, to initiate thrombi formation.
Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism (1988)

Sterol uptake control mutants (upc-) have been isolated via ethylmethanesulfonate mutagenesis from wild-type Saccharomyces cerevisiae. These mutants are heme and sterol competent but possess the ability to accumulate exogenous sterol(s) under aerobic conditions. Previous studies demonstrate sterol uptake only in a hem-, erg- background; however, the Upc- strains described here are Hem+ and do not require exogenous sterol for growth. We were unable to obtain viable hem+, erg-, upc+ recombinants; such combinations appear to be lethal. Isolates of Upc mutants demonstrated different levels of sterol uptake, and sterol analysis revealed a broad phenotypic range with regard to amounts and accumulation of ergosterol and non-ergosterol sterols. Assays of acyl CoA: ergosterol acyltransferase and sterol ester hydrolase showed no apparent difference in activity between Upc mutants and the wild type.
Dosimetric uncertainties of three-dimensional dose reconstruction from two-dimensional data in a multi-institutional study (2004)

Inconsistencies in the treatment planning process leading to dosimetric uncertainties may affect conclusions drawn from interinstitutional radiation oncology clinical trials. The purpose of this study was to assess the dosimetric uncertainties resulting from the process of reconstructing three-dimensional dose distributions from two-dimensional treatment plan information provided by participating institutions in a randomized clinical trial. This study was based on American College of Radiology Protocol #427, Locally Advanced Multi-Modality Protocol; a multi-institutional phase II randomized study involving radiation therapy for patients with inoperable non-small cell lung cancer. Several sources of dosimetric uncertainty were identified and analyzed, including image quality of hard-copy computed tomography (CT) images, slice spacing of CT scans, treatment position, interpretations of target volumes by radiation oncologists, the contouring of normal anatomic structures, and the use of common beam models for all dose calculations. Each source of uncertainty was investigated using a set of plans, with the ideal characteristics of digital images with 3-mm axial slice spacing and a flat couch, consisting of eight cases from Vanderbilt University Medical Center with electronically transferred CT data. The target volume DVH values were dependent on the additional uncertainty introduced by differences in delineation of the target volumes by the participating radiation oncologists. The DVH values for the lungs and heart were dependent on image quality and treatment position. Esophagus DVH values were not dependent on any of the sources of uncertainty. None of the structure DVH values were dependent on slice thickness or variations in the contouring of normal anatomic structures. Reconstruction of three-dimensional dose distributions from two-dimensional treatment plan information may be useful in cases for which digital CT data is not available or for historical data review. However, dosimetric accuracy will depend on image quality of the treatment planning CT data and consistency in the delineation of tumor volumes. PACS number: 87.53.-j
Experimental and Clinical Regenerative Capability of Human Bone Marrow Cells After Myocardial Infarction (2004)

Bone marrow mononuclear cells (BMCs) from 20 patients with extensive reperfused myocardial infarction (MI) were used to assess their myocardial regenerative capability "in vitro" and their effect on postinfarction left ventricular (LV) remodeling. Human BMCs were labeled, seeded on top of cryoinjured mice heart slices, and cultured. BMCs showed tropism for and ability to graft into the damaged mouse cardiac tissue and, after 1 week, acquired a cardiomyocyte phenotype and expressed cardiac proteins, including connexin43. In the clinical trial, autologous BMCs (78+/-41x10(6) per patient) were intracoronarily transplanted 13.5+/-5.5 days after MI. There were no adverse effects on microvascular function or myocardial injury. No major cardiac events occurred up to 11+/-5 months. At 6 months, magnetic resonance showed a decrease in the end-systolic volume, improvement of regional and global LV function, and increased thickness of the infarcted wall, whereas coronary restenosis was only 15%. No changes were found in a nonrandomized contemporary control group. Thus, BMCs are capable of nesting into the damaged myocardium and acquire a cardiac cell phenotype in vitro as well as safely benefiting ventricular remodeling in vivo. Large-scale randomized trials are needed now to assess the clinical efficacy of this treatment.