gebhart/patrepeval huggingface dataset:
Supported lipid bilayer microarrays created by non-contact printing (2011)

Arrays of supported lipid bilayers (SLBs) provide great potential for future drug development and multiplexed biological research, but are difficult to prepare due to the sensitivity of both the lipid and protein structural arrangement to air exposure. A novel way to produce arrays of SLBs is presented based on non-contact dispensing of vesicles to a substrate through a thin surface confined water film. The approach presents many degrees of freedom since it is not limited to a specific substrate, lipid composition, linker or controlled environment. The method allows adjustment of spot size (180-360 μm) by repeated dispensing as well as control over the composition of the spots and subsequent analytes. SLB formation by vesicle adsorption and rupture allows for incorporation of membrane proteins through pre-formed proteoliposomes. Dispensing through a dip-and-rinse water film avoids contamination, disruptive drying and the need for complex buffer compositions. Furthermore, no humidity control is necessary which simplifies the production step and prolongs the life-time of the spotting system. We characterize the method with respect to control over spot size, bilayer mobility and the formation process as well as demonstrate the possibility to fuse bilayer spots with subsequently added vesicles. Since complex lipid compositions and multiple spotting nozzles can be used, this novel technique is expected to be a promising platform for future applications, e.g. patterning to monitor peptide/protein-lipid interactions, for glycomics using glycolipids or lipopolysaccharides, and to study mixing of spatially confined lipid membranes.
Complement activation by beta-amyloid in Alzheimer disease. (1992)

Alzheimer disease (AD) is characterized by excessive deposition of the beta-amyloid peptide (beta-AP) in the central nervous system. Although several lines of evidence suggest that beta-AP is neurotoxic, a mechanism for beta-AP toxicity in AD brain remains unclear. In this paper we provide both direct in vitro evidence that beta-AP can bind and activate the classical complement cytolytic pathway in the absence of antibody and indirect in situ evidence that such actions occur in the AD brain in association with areas of AD pathology.
Research of High-voltage Power Frequency Electric Field Warning Instrument (2009)

Environment impact tests both under the high-voltage transmission lines and in the high-voltage substations indicate that the electric field strength in some places exceeds 5kV/m which is the allowable value of national standard. In order to help power workers to get the electric field information around the working place accurately and immediately, a power frequency electric field warning instrument is designed based on C8051F020 single-chip. Experiments have shown that the instrument can not only measure the power frequency electric field, but also warn automatically when the electric field strength in the environment is larger than the standard authorized value. It can meet the demand to monitor the electric field environment in power system.
LAG-3 regulates CD8+ T cell accumulation and effector function in murine self- and tumor-tolerance systems (2007)

Lymphocyte activation gene-3 (LAG-3) is a cell-surface molecule with diverse biologic effects on T cell function. We recently showed that LAG-3 signaling is important in CD4+ regulatory T cell suppression of autoimmune responses. Here, we demonstrate that LAG-3 maintains tolerance to self and tumor antigens via direct effects on CD8+ T cells using 2 murine systems. Naive CD8+ T cells express low levels of LAG-3, and expression increases upon antigen stimulation. Our data show increased levels of LAG-3 protein on antigen-specific CD8+ T cells within antigen-expressing organs or tumors. In vivo antibody blockade of LAG-3 or genetic ablation of the Lag-3 gene resulted in increased accumulation and effector function of antigen-specific CD8+ T cells within organs and tumors that express their cognate antigen. Most notably, combining LAG-3 blockade with specific antitumor vaccination resulted in a significant increase in activated CD8+ T cells in the tumor and disruption of the tumor parenchyma. A major component of this effect was CD4 independent and required LAG-3 expression by CD8+ T cells. Taken together, these data demonstrate a direct role for LAG-3 on CD8+ T cells and suggest that LAG-3 blockade may be a potential cancer treatment.
A monolithically integrated detector-preamplifier on high-resistivity silicon (1990)

A monolithically integrated detector-preamplifier on high-resistivity silicon has been designed, fabricated, and characterized. The detector is a fully depleted p-i-n diode, and the preamplifier is implemented in a depletion-mode PMOS process which is compatible with detector processing. The amplifier is internally compensated, and the measured gain-bandwidth product is 30 MHz with an input-referred noise of 15 nV/ square root Hz in the white-noise regime. Measurements with an Am/sup 241/ radiation source yield an equivalent input noise charge of 800 electrons at 200 ns shaping time for a 1.4 mm/sup 2/ detector with on-chip amplifier in an experimental setup with substantial external pickup.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Metrics for analyzing module interactions in large software systems (2005)

We present a new set of metrics for analyzing the interaction between the modules of a large software system. We believe that these metrics would be important to any automatic or semi-automatic code modularization algorithm. The metrics are based on the rationale that code partitioning should be based on the principle of similarity of service provided by the different functions encapsulated in a module. Although module interaction metrics are necessary for code modularization, in practice they must be accompanied by metrics that measure other important attributes of how the code is partitioned into modules. These other metrics, dealing with code properties such as the approximate uniformity of module sizes, conformance to any size constraints on the modules, etc., are also included in the work presented here. To give the reader some insight into the workings of our metrics, this paper also includes some results obtained by applying the metrics to the body of code that constitutes the open-source Apache HTTP server. We apply our metrics to this code as packaged by the developers of the software and to the other partially and fully randomized versions of the code.
Thyroid imaging using positron emission tomography—a comparison with ultrasound imaging and conventional scintigraphy in thyrotoxicosis (1990)

Forty-six comparative studies were performed on 41 patients with hyperthyroidism. Clinically these comprised two groups: those with Graves' disease, and those with multinodular goitre. All patients underwent an ultrasound examination and positron emission tomography (PET) using 124I, then gamma camera pinhole imaging following their 131I therapy administration. Although the 131I pinhole imaging was not performed for diagnostic purposes, there was good correlation (78% agreement) between it and 124I PET in determining relative lobe size. Hence either imaging modality could be used as an indicator of the relative radiation dose delivered to each thyroid lobe at a macroscopic level. In terms of gland morphology the PET images corresponded well to the high resolution ultrasound images (78% agreement), unlike the pinhole images which correlated poorly (only 28% agreement). The results showed that PET imaging gives better anatomical and physiological detail than 131I pinhole imaging. In 77% of cases where the pinhole image showed a uniform distribution of radioisotope, the improved spatial resolution of the PET images revealed non-homogeneous distribution indicating a non-uniform distribution of radiation dose. Since all dosimetry calculations are based on the assumption of uniform distribution of radioiodine, this non-uniformity could possibly have important consequences in the outcome of radioiodine therapy in thyrotoxicosis.
A simple algorithm for the control of reactances in beam steering applications with parasitic elements (2004)

A simple algorithm for the control of reactances in parasitic circular aerial beamforming arrays is presented. The computation of the capacitances is straightforward and eliminates the use of optimization procedures. Different sets of capacitances for rotating-beam applications are easily obtained. The beam shape is maintained during rotation.
Endosperm‐specific activity of a storage protein gene promoter in transgenic wheat seed (2001)

The characterization of the promoter of a wheat (Triticum aestivum) cv. Cheyenne high molecular weight glutenin subunit (HMW subunit) gene, Glu‐1D‐1 is reported. The nucleotide sequence of the promoter from position −1191 to −650 with respect to the transcription start site was determined, to add to that already determined. Analysis of this region of the promoter revealed the presence of an additional copy of part of the primary enhancer sequence and sequences related to regulatory elements present in other wheat seed protein genes. A chimaeric gene was constructed comprising the 5′ flanking region of the Glu‐1D‐1 gene from position −1191 to +58, the coding region of the UidA (Gus) gene, and the nopaline synthase (Nos) gene terminator. This chimaeric gene was introduced into wheat (Triticum durum cv. Ofanto) by particle bombardment of inflorescence explants. Two independent transgenic lines were produced, and both showed expression of the Gus gene specifically in the endosperm during mid‐development (first detected 10–12 d after anthesis). Histochemical analysis of homozygous T2 seed confirmed this pattern of expression, and showed that expression was initiated first in the central lobes of the starchy endosperm, and then spread throughout the endosperm tissue, while no expression was detected in the aleurone layer. Native HMW subunit protein was detectable by Western analysis 12–14 d after anthesis, consistent with concurrent onset of activity of the native and introduced HMW subunit gene promoters.
Write a Classifier: Predicting Visual Classifiers from Unstructured Text (2016)

People typically learn through exposure to visual concepts associated with linguistic descriptions. For instance, teaching visual object categories to children is often accompanied by descriptions in text or speech. In a machine learning context, these observations motivates us to ask whether this learning process could be computationally modeled to learn visual classifiers. More specifically, the main question of this work is how to utilize purely textual description of visual classes with no training images, to learn explicit visual classifiers for them. We propose and investigate two baseline formulations, based on regression and domain transfer, that predict a linear classifier. Then, we propose a new constrained optimization formulation that combines a regression function and a knowledge transfer function with additional constraints to predict the parameters of a linear classifier. We also propose a generic kernelized models where a kernel classifier is predicted in the form defined by the representer theorem. The kernelized models allow defining and utilizing any two Reproducing Kernel Hilbert Space (RKHS) kernel functions in the visual space and text space, respectively. We finally propose a kernel function between unstructured text descriptions that builds on distributional semantics, which shows an advantage in our setting and could be useful for other applications. We applied all the studied models to predict visual classifiers on two fine-grained and challenging categorization datasets (CU Birds and Flower Datasets), and the results indicate successful predictions of our final model over several baselines that we designed.
Structure and expression of the human L-myc gene reveal a complex pattern of alternative mRNA processing. (1988)

We analyzed in detail the structure of the L-myc gene isolated from human placental DNA and characterized its expression in several small-cell lung cancer cell lines. The gene is composed of three exons and two introns spanning 6.6 kilobases in human DNA. Several distinct mRNA species are produced in all small-cell lung cancer cell lines that express L-myc. These transcripts are generated from a single gene by alternative splicing of introns 1 and 2 and by use of alternative polyadenylation signals. In some mRNAs there is a long open reading frame with a predicted translated protein of 364 residues. Amino acid sequence comparison with c-myc and N-myc demonstrated multiple discrete regions with extensive homology. In contrast, other mRNA transcripts, generated by alternative processing, could encode a truncated protein with a novel carboxy-terminal end.
Microbial degradation of complex carbohydrates in the gut (2012)

Bacteria that colonize the mammalian intestine collectively possess a far larger repertoire of degradative enzymes and metabolic capabilities than their hosts. Microbial fermentation of complex non-digestible dietary carbohydrates and host-derived glycans in the human intestine has important consequences for health. Certain dominant species, notably among the Bacteroidetes, are known to possess very large numbers of genes that encode carbohydrate active enzymes and can switch readily between different energy sources in the gut depending on availability. Nevertheless, more nutritionally specialized bacteria appear to play critical roles in the community by initiating the degradation of complex substrates such as plant cell walls, starch particles and mucin. Examples are emerging from the Firmicutes, Actinobacteria and Verrucomicrobium phyla, but more information is needed on these little studied groups. The impact of dietary carbohydrates, including prebiotics, on human health requires understanding of the complex relationship between diet composition, the gut microbiota and metabolic outputs.
Functional analyses of the cancer stem cell-like properties of human endometrial tumor initiating cells (2008)

Recent data suggest that rare stem cell populations with the capacity to self renew and drive tumor formation are a feature of solid tumors. Several investigators have identified putative stem cells from solid tumors and cancer cell lines following isolation of a side population (SP) defined by dye exclusion. We investigated this parameter in our efforts to identify an endometrial cancer (EnCa) stem cell population. Multiple EnCa cell lines were assessed and verapamil sensitive SP and non-SP cells were isolated from two human EnCa cell lines. The functional significance of the SP and non-SP derived from AN3CA was evaluated in vitro and in vivo. SP cells proliferated at a significantly slower rate than the non-SP fraction, and a larger proportion of the SP cells were in the G(1) phase of the cell cycle as compared to the non-SP fraction. The SP fraction was more resistant to the chemotherapeutic agent paclitaxel. The SP comprised -0.02% of the initial AN3CA cell population and this proportion of SP cells was maintained within the larger heterogeneous population following repeated passages of purified SP cells. These findings suggest that SP cells derived from the An3CA cell line have the stem cell properties of low proliferative activity, chemoresistance and self-renewal. We also tested relative tumor formation activity of the SP and non-SP fractions. Only the SP fraction was tumorigenic. Additionally, we identified SP fractions in primary EnCa. Together these results are consistent with the hypothesis that EnCa contain a subpopulation of tumor initiating cells with stem like properties.
Thermal conductivity of micromachined low-stress silicon-nitride beams from 77 to 325 K (2009)

We present thermal conductivity measurements of micromachined 500 nm thick silicon-nitride (Si–N) beams suspended between two Si–N islands, in the temperature range from 77 to 325 K. The measured thermal conductivity, k, of Si–N at high temperatures is in good agreement with previously measured values for Si–N grown by low-pressure chemical vapor deposition, but behaves much differently as temperature is lowered, showing a dependence more similar to polycrystalline materials. Preliminary structural characterization by x-ray diffraction suggests that the material is likely nano- or polycrystalline. The micromachined suspended platform structure is designed to allow highly accurate measurements of the thermal conductivity of deposited metallic, semiconducting, or insulating thin films. As a demonstration, we present measurements of a 200 nm thick sputtered molybdenum film. In the entire temperature range the measured thermal conductivity matches the prediction of the Wiedemann–Franz thermal conductivity determined from measured electrical conductivity.
Tungsten carbene complexes in olefin metathesis: a cationic and chiral active species (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTungsten carbene complexes in olefin metathesis: a cationic and chiral active speciesJacky Kress and John A. OsbornCite this: J. Am. Chem. Soc. 1983, 105, 20, 6346–6347Publication Date (Print):September 1, 1983Publication History Published online1 May 2002Published inissue 1 September 1983https://pubs.acs.org/doi/10.1021/ja00358a044https://doi.org/10.1021/ja00358a044research-articleACS PublicationsRequest reuse permissionsArticle Views427Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Microglia and inflammatory mechanisms in the clearance of amyloid β peptide (2002)

There is now abundant evidence that brain microglia, when activated, have the lineage, receptors, and synthetic capacity to participate in both potentially neurotoxic inflammatory responses and potentially beneficial phagocytic responses. Amyloid beta peptide (Abeta) forms highly insoluble, beta-pleated aggregates that are widely deposited in the Alzheimer's disease (AD) cortex and limbic system. Aggregated Abeta also activates the classical and alternative complement cascades. These properties make Abeta an excellent target for microglial phagocytosis, a view supported by multiple reports, through well established mechanisms of phagocyte clearance.
Inhibitory effects of acyclic nucleoside phosphonates on human hepatitis B virus and duck hepatitis B virus infections in tissue culture (1994)

The inhibitory effects of the 9-(2-phosphonylmethoxyethyl)adenine-related compounds (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)-adenine, (S)-9-(3-fluoro-2-phosphonylmethoxypropyl)adenine, (R)-9-(2-phosphonylmethoxypropyl)adenine, (R)-9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine, and (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine on human hepatitis B virus replication in the human hepatoma cell line HepG2 2.2.15 and duck hepatitis B virus infection in primary duck hepatocytes were investigated. (R)-9-(2-phosphonylmethoxypropyl-2,6-diaminopurine had the lowest 50% inhibitory concentrations against hepatitis B virus and duck hepatitis B virus, 0.22 and 0.06 microM, respectively, i.e., two- to fivefold lower concentrations than required for (R)-9-(2-phosphonylmethoxypropyl)adenine and 9-(2-phosphonylmethoxyethyl)adenine. All compounds were not toxic in vitro at a concentration of 100 microM.
Neurology of ciguatera (2001)

Ciguatera is a widespread ichthyosarcotoxaemia with dramatic and clinically important neurological features. This severe form of fish poisoning may present with either acute or chronic intoxication syndromes and constitutes a global health problem. Ciguatera poisoning is little known in temperate countries as a potentially global problem associated with human ingestion of large carnivorous fish that harbour the bioaccumulated ciguatoxins of the photosynthetic dinoflagellate Gambierdiscus toxicus. This neurotoxin is stored in the viscera of fish that have eaten the dinoflagellate and concentrated it upwards throughout the food chain towards progressively larger species, including humans. Ciguatoxin accumulates in all fish tissues, especially the liver and viscera, of "at risk" species. Both Pacific (P-CTX-1) and Caribbean (C-CTX-1) ciguatoxins are heat stable polyether toxins and pose a health risk at concentrations above 0.1 ppb. The presenting signs of ciguatera are primarily neurotoxic in more than 80% of cases. Such include the pathognomonic features of postingestion paraesthesiae, dysaesthesiae, and heightened nociperception. Other sensory abnormalities include the subjective features of metallic taste, pruritus, arthralgia, myalgia, and dental pain. Cerebellar dysfunction, sometimes diphasic, and weakness due to both neuropathy and polymyositis may be encountered. Autonomic dysfunction leads to hypotension, bradycardia, and hypersalivation in severe cases. Ciguatoxins are potent, lipophilic sodium channel activator toxins which bind to the voltage sensitive (site 5) sodium channel on the cell membranes of all excitable tissues. Treatment depends on early diagnosis and the early administration of intravenous mannitol. The early identification of the neurological features in sentinel patients has the potential to reduce the number of secondary cases in cluster outbreaks.