gebhart/patrepeval huggingface dataset:
ORGANIZATIONAL LEARNING AND SENGE'S FIVE DISCIPLINES: ARESEARCH ON THE FLORIANOPOLIS/SC AIR FORCE MILITARY BAND (2009)

This paper aims to verify in general a Band of Music, a
					strictly military organization, in this case the Band Music of the
					Air Base of Florianopolis, propelling the development of practices
					of organizational learning. It is about a descriptive case research
					primarily quantitative, with questionnaires, in order to verify
					that the environment of a military organization provides to your
					actors satisfactory levels of organizational learning. To answer
					the purpose of research, were established as categories of analysis
					the five disciplines, conceptualized by Peter Senge and practices
					leading to the learning process occurs at the institutional level.
					The methodology of applied research also incorporated qualitative
					techniques of participant observation and document analysis, they,
					in turn, served to raise a brief history of the Air Base and the
					Band of Music - but also check the structure of the band. Moreover,
					the listed techniques comply with quantitative results. It was
					testified that the music band structure is categorized as linear,
					for presenting this kind of assortment: linear authority, formal
					communication line, decision centralization and pyramidal aspects.
					The categories of analysis used to check the levels of
					organizational learning, as satisfactory or unsatisfactory were:
					personal mastery, mental models, building shared vision, team
					learning and systemic thinking in. These categories were
					conceptualized by Senge (1990), as practices that are widespread in
					the organization, promoting a learning environment. The categories
					were further subdivided into units of analysis. In general there
					was a predominance of factors that enable the development of
					learning in organizational levels in the Military Band Music. These
					results are based on statistical analysis of data collected,
					according to the criteria outlined previously established by the
					conceptual scope of theoretical research and detail that are
					presented in this article.
Determination of the Time Course and Extent of Neurotoxicity at Defined Temperatures in Cultured Neurons Using a Modified Multiwell Plate Fluorescence Scanner (1997)

The cellular and molecular mechanisms of hypoxic/ischemic neurodegeneration are sensitive to numerous factors that modulate the time course and degree of neuronal death. Among such factors is hypothermia, which can dramatically protect neurons from injury. To examine and control for temperature-dependent effects, we developed a technique that provides for a high-throughput, accurate, and reproducible determination of the time course and degree of neurotoxicity in cultured cortical neurons at precisely defined temperatures. We used a fluorescence multiwell plate scanner, modified by us to permit the control of temperature, to perform serial quantitative measurements of propidium iodide (PI) fluorescence in cortical neuronal cultures exposed to excitotoxic insults. In validating this approach, we show that these time course measurements correlate highly with manual counts of PI-stained cells in the same cultures (r = 0.958, p < 0.0001) and with lactate dehydrogenase release (r = 0.964, p < 0.0001). This method represents an efficient approach to mechanistic and quantitative studies of cell death as well as a high-throughput technique for screening new neuroprotective therapies in vitro.
Three-dimensional imaging of the stomach: role of pylorus in the emptying of liquids (1987)

Using the dynamic spatial reconstructor and manometry, we measured gastric emptying, pyloric size, and antral contractile activity in three anesthetized dogs in the prone position. Our objective is to evaluate the effect of intestinal perfusion of equicaloric, neutral, and isosmolar nutrients (Maltose 32.5 mg/ml, casein hydrolysate 32.5 mg/ml, and oleic acid 15.5 mg/ml) at a constant rate of 10 ml/min in the proximal jejunum on the dynamics of the antropyloric region. Isotonic saline perfusion was used as a control solution. Test meal in the stomach was 600 ml of isotonic Gastrograffin. Antral phasic pressure activity was recorded using an antroduodenal six-channel (ports 5 mm apart) perfused probe (2 mm OD). Gastric emptying in response to intestinal perfusion of fat was significantly slower (P less than 0.02) in comparison to other nutrients or isotonic saline. There was no significant difference in the maximum or minimum pyloric diameter in response to the four intestinal perfusates. However, the pylorus was closed for a significantly longer duration (P less than 0.05) during the perfusion of fat in the upper intestine. Antral contractions were related to pyloric opening in an "antral contraction followed by pyloric opening" sequence. This study suggests that the presence of fat in the upper intestine delays gastric emptying and that this effect is regulated in part by increased resistance to flow offered by the pylorus.
Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes (1984)

Varicella-zoster virus (VZV) codes for approximately eight glycosylated polypeptides in infected cell cultures and in virions. To determine the number of serologically distinct glycoprotein gene products encoded by VZV, we have developed murine monoclonal antibodies to purified virions. Of 10 monoclonal antibodies which can immunoprecipitate intracellular VZV antigens and virion glycoproteins, 1 (termed gA) reacted with gp105, 1 (termed gB) reacted with gp115 (intracellular only), gp62, and gp57, and 8 (termed gC) reacted with gp92, gp83, gp52, and gp45. The anti-gA monoclonal antibody neutralized VZV infectivity in the absence of complement. All eight anti-gC monoclonal antibodies neutralized only in the presence of complement. An anti-gB monoclonal antibody obtained from another laboratory also neutralizes in the absence of complement. Since the above reactivities account for all major detectable VZV glycoprotein species, the data strongly suggest that VZV has three major glycoprotein genes which encode glycosylated polypeptides with neutralization epitopes.
A high accuracy camera calibration for vision-based measurement systems (2004)

An important task in most 3D measurement systems based on machine vision is camera calibration, whose objective is to estimate the internal and external parameters of each camera. A new accurate calibration method with multilevel process of camera parameter is presented. Flexibly making use of geometry imaging theory, our algorithm obtain all the parameters through logical organization of solving order, accordingly avoid obtaining possible local optimized problem when solving the non-linear equation. The camera model is aimed at efficient computation of camera extrinsic and intrinsic parameters considering lens distortion, which are solved dividedly. Built on strict geometry constraint, our calibration method gets over the relativity influence of every unknown parameters of traditional calibration way, and makes the error distributed among the constraint relation of parameters, in order to guarantee the accuracy. Experimental results are provided to show that the accuracy is high.
Separation of sequences defining basal expression from those conferring alpha gene recognition within the regulatory domains of herpes simplex virus 1 alpha genes. (1984)

The genes of herpes simplex virus 1 form three major groups--alpha, beta, and gamma--whose expression is coordinately regulated and sequentially ordered in a cascade fashion. To determine how the infected cell differentiates between these gene groups, alpha-regulated chimeric genes were constructed in earlier studies by fusing the structural sequences of the thymidine kinase (TK) gene, a beta gene, to the 5' noncoding sequences of alpha genes. These studies showed that (i) one or more structural components of the virion act in trans to increase alpha gene expression and (ii) the 5' noncoding sequences of alpha genes contain cis-acting domains that promote gene expression and confer alpha-gene regulation. These two domains could be moved independently, but the regulatory domain required a promoter for its function. We report here the properties of three sequences containing features common to the regulatory regions of all alpha genes. Sequence 1, containing (G + C)-rich inverted repeats, increased the basal level of TK expression when fused 5' to either the alpha gene 4 promoter or the truncated beta TK promoter. The effect was to some extent orientation dependent. Moreover, sequence 1 restored beta regulation to the truncated beta TK promoter but did not confer alpha-specific regulation on any of the chimeric genes tested. Sequences 2 (49 base pairs) and 3 (29 base pairs), containing an (A + T)-rich homolog from alpha gene 27 and alpha gene 0, respectively, restored alpha-specific regulation to the alpha promoter gene but only sequence 2 conferred alpha regulation on the truncated beta promoter gene. Our results indicate that (i) in natural beta TK the promoter and regulatory domains overlap, (ii) sequence 1 determines basal level of expression and substitutes for a promoter component that is essential for beta but not alpha regulation, and (iii) conversion of a gene with a promoter into an alpha gene requires two elements. Sequence 2 may contain both whereas sequence 3 contains only one.
[Role of antineuraminidase antibodies in protection against influenza]. (1998)

For improving the anti-influenza vaccination efficacy, the choice of strains carrying up dated neuraminidase antigen (NA) and the introduction of the optimal amount of NA antigen in the vaccine are critical. Monoclonal antibodies prepared against the neuraminidase N2 of A/Beijing/32/92 showed NA inhibition (NI) and neutralized (Nt) the cells infection by influenza virus either at an early stage (group 2 antibodies inhibit virus binding to cells) or at a late stage of infection (group 1 antibodies inhibit virus release). The specificity of the neutralization test is restricted to the homologous variant whereas the NI specificity is much broader. When both group 1 and group 2 antibodies are tested together, their neutralizing activity is significantly increased. The emergence in 1997 of an avian strain H5N1 in humans influenza infections at Hong Kong (Strain A/Hong-Kong/156/97) rose the threat of pandemic. The H5N1 strain carried H5 HA which is not recognized by the human immune system, but N1 might be related to other N1 antigens belonging to avian, swine and human strains. So we 1) characterized the N1 antigen from H5N1 in comparison with other known antigens, 2) we looked for anti N1 (H5N1) antibodies in humans according to the age and the vaccination status, 3) we checked the neutralizing activity of anti N1 antibodies. The N1 antigen (H5N1) appeared closely related to N1 from swine strains: Sw/31 correlated itself to the pandemic spanish virus (1918-19), and more recent swine isolates from 1982 and 1989. The anti N1 (H5N1) antibodies were present in sera collected from 75+ years old persons and these N1 antibodies were neutralizing H5N1 cells infection. Consequently, 75+ years old persons do not represent a priority group for vaccination in the case of H5N1 pandemic conditions.
High performance 600 V smart power technology based on thin layer silicon-on-insulator (2002)

A high-performance 600 V smart power technology has been developed in which novel lateral double-diffused MOS transistors (LDMOS) are merged with a BiCMOS process flow for the construction of power integrated circuits on bonded silicon-on-insulator (BSOI) substrates. All active and passive device structures have been optimized for fabrication on BSOI layers which are less than 1.5 /spl mu/m-thick, with buried oxide layers in the range of 2.0 to 3.0 /spl mu/m-thick. Complete dielectric isolation processing is straightforward due to the use of a thin SOI active device layer. A dual field plate design of the high-voltage devices results in at least a factor-of-two reduction in specific on-resistance over conventional LDMOS structures for a given breakdown voltage.
Gesundheitsförderung im Handwerk (1992)

Gesundheitsförderung, Handwerk, Kleinbetrieb, Gesundheitsbericht, Gesundheitszustand, Arbeitnehmer, Arbeitsbelastung, abhängig Beschäftigter, EDV-Programm, Krankenkasse, Bundesrepublik Deutschland
The NMDA Receptor antagonists, LY274614 and MK-801, and the nitric oxide synthase inhibitor, NG-nitro-L-arginine, attenuate analgesic tolerance to the mu-opioid morphine but not to kappa opioids (1994)

Once daily s.c. administration of 5 mg/kg morphine, a mu-opioid agonist, or U50488H (U50), a kappa 1-opioid agonist, for 5 days in male CD-1 mice results in a 2-3-fold shift to the right of the respective analgesic (tail flick) dose-response curves, indicating the development of tolerance. Concurrent s.c. administration of the competitive NMDA receptor antagonist, LY274614 (LY), at 24 mg/kg/24 h infusion (osmotic pump) or 6 mg/kg i.p. once daily attenuates the development of morphine tolerance, when the response to saline plus morphine is compared on day 5 with LY plus morphine. Using this paradigm, once daily administration of either the non-competitive NMDA antagonist, MK-801, at 0.3 mg/kg i.p. or the nitric oxide synthase inhibitor, NG-nitro-L-arginine (NorArg), at 1 mg/kg i.p. twice daily attenuated the development of morphine tolerance. None of these drugs modify the tail-flick response or alter the ED50 for morphine. In contrast, co-administration of LY, MK-801 or NorArg, as above, failed to attenuate the development of tolerance to U50 or to the kappa 3-opioid agonist, naloxone benzoylhydrazone (NalBzoH). These results suggest that mu-opioid tolerance but not kappa 1- or kappa 3-opioid tolerance involves the mediation of NMDA receptors and the nitric oxide system.
A magnetic internal mechanism for precise orientation of the camera in wireless endoluminal applications (2010)

We report the feasibility and effectiveness of the combined use of external static magnetic fields and internal actuation to move small permanent intracapsular magnets to achieve wirelessly controllable and precise camera steering. The concept is applicable to capsule endoscopy as to other instrumentation for laparoscopic, endoluminal, or transluminal procedures.
HLA Class I Antigen Processing Machinery Component Expression and Intratumoral T-Cell Infiltrate as Independent Prognostic Markers in Ovarian Carcinoma (2008)

The negative effect of APM component down-regulation by itself and in combination with absent intratumoral T-cell infiltration on the survival of patients with ovarian carcinoma implies a role for immune escape in addition to immunosurveillance in the clinical course of disease.
216-02: In vitro analysis of gas bubble formation and its effect on impedance during electroporation ablation (2016)

Introduction: Irreversible electroporation via a multi-electrode circular catheter is a novel method for cardiac ablation. Due to electrolysis, gas formation will occur at the electrode surfaces that eventually may lead to arcing. We hypothesized that the formation of electrically isolating gas bubbles on the electrode surface can be detected by a change in electrical resistance. In this in vitro study we investigated the relationship between electrolytic gas formation and electrical impedance using various energy levels and catheter hoop diameters. Methods: In a tank filled with physiologic saline, 200 - 360 J was applied between a 14 pole circular electrode catheter with 2.5 mormation was assessed using ultra-high-speed video recordings (10k fps) while synchronously recording voltage and current waveforms. Conclusion: Decreasing the hoop diameter of a catheter leads to a higher impedance and larger gas bubbles. When using a hoop diameter, lower energies should be applied.
PECAM-1 is expressed on hematopoietic stem cells throughout ontogeny and identifies a population of erythroid progenitors (2004)

Platelet endothelial cell adhesion molecule-1 (PECAM-1) (CD31) is an adhesion molecule expressed on endothelial cells and subsets of leukocytes. Analysis of phenotypically defined hematopoietic stem cells (HSCs) from the yolk sac, fetal liver, and adult bone marrow demonstrates CD31 expression on these cells throughout development. CD31+ c-kit+ cells, but not CD31- c-kit+ cells, isolated from day-9.5 yolk sac give rise to multilineage hematopoiesis in vivo. Further evaluation of the CD31+ lineage marker-negative fraction of adult bone marrow reveals functionally distinct cell subsets. Transplantation of CD31+ Lin- c-kit- cells fails to protect lethally irradiated recipients, while CD31+ Lin- c-kit+ Sca-1- cells (CD31+ Sca-1-) provide radioprotection in the absence of long-term donor-derived hematopoiesis. Although donor-derived leukocytes were not detected in CD31+ Sca-1- recipients, donor-derived erythroid cells were transiently produced during the initial phases of bone marrow recovery. These results demonstrate CD31 expression on hematopoietic stem cells throughout ontogeny and identify a population of CD31+ short-term erythroid progenitors cells that confer protection from lethal doses of radiation.
Multilevel evaluation of electromagnetic fields for the rapid solution of scattering problems (1994)

Abstract A multilevel algorithm is presented for evaluating fields generated by given distributions of electric current. The algorithm can be used to accelerate the solution of scattering problems using the method of moments and/or boundary element methods. The algorithm exploits the limited number of degrees of freedom that characterize a field observed over a domain that is well separated from a source domain. Both the memory requirements and the computational complexity of the algorithm are O(N s log(N s )), where N s is the common number of sources and observation points. The multilevel algorithm can easily be incorporated into existing method‐of‐moments (MoM) programs. A simple physical interpretation of the algorithm is provided. © 1994 John Wiley &amp; Sons, Inc.
Dynamic resource allocation with management objectives: implementation for an OpenStack cloud (2012)

We report on design, implementation and evaluation of a resource management system that builds upon OpenStack, an open-source cloud platform for private and public clouds. Our implementation supports an Infrastructure-as-a-Service (IaaS) cloud and currently provides allocation for computational resources in support of both interactive and computationally intensive applications. The design supports an extensible set of management objectives between which the system can switch at runtime. We demonstrate through examples how management objectives related to load-balancing and energy efficiency can be mapped onto the controllers of the resource allocation subsystem, which attempts to achieve an activated management objective at all times. The design is extensible in the sense that additional objectives can be introduced by providing instantiations for generic functions in the controllers. Our implementation monitors the fulfillment of the relevant management metrics in real time. Testbed evaluation demonstrates the effectiveness of our approach in a dynamic environment. It further illustrates the trade-off between closely meeting a specific management objective and the associated cost of VM live-migration.
Studies on 15-Hydroxyprostaglandin Dehydrogenase with Various Prostaglandin Analogues (1978)

The NAD+-linked 15-hydroxyprostaglandin dehydrogenase (PGDH) of swine lung was purified to a high specific activity by affinity chromatographies on prostaglandin (PG)-and NAD+-Sepharose. The affinities of the enzyme for various synthetic analogues of PGA, E, F, and I and their inhibitory effects on the enzymatic reaction were examined. The modification of the alkyl side chain of PG, particularly at C-15 or C-16, reduced the affinity of the enzyme for these PG analogues. Furthermore, 14-methyl-13,14-dihydro-PGE1 and 16-cyclopentyl-omega-trinor-15-epi-PGE2 were potent inhibitors of PGDH.
180-kD ribosome receptor is essential for both ribosome binding and protein translocation. (1993)

We have previously isolated a 180-kD ribosome receptor (p180) from mammalian rough ER that, when incorporated into liposomes, bound ribosomes with an affinity similar to intact membranes. To directly assess the contribution of p180 to ribosome binding as well as protein translocation, monoclonal antibodies were used to selectively deplete p180 from the detergent extracts of rough ER membranes used in the preparation of translocation-competent proteoliposomes. Proteoliposomes prepared from p180-depleted extracts showed a reduction in ribosome binding to the level of trypsin-inactivated controls as well as a loss in their ability to cotranslationally translocate two different secretory protein precursors. When purified p180 was added back to depleted extracts before proteoliposome formation, both ribosome binding and translocation activity were restored. In addition, the monoclonal antibodies, as well as their Fab' fragments, were able to inhibit ribosome binding and protein translocation when bound to intact rough microsomes. These data provide direct evidence that the 180-kD ribosome receptor is essential for ribosome binding and for the translocation of nascent proteins across the membrane of the rough ER.
Characterization of Chimpanzee/Human Monoclonal Antibodies to Vaccinia Virus A33 Glycoprotein and Its Variola Virus Homolog In Vitro and in a Vaccinia Virus Mouse Protection Model (2007)

Three distinct chimpanzee Fabs against the A33 envelope glycoprotein of vaccinia virus were isolated and converted into complete monoclonal antibodies (MAbs) with human gamma 1 heavy-chain constant regions. The three MAbs (6C, 12C, and 12F) displayed high binding affinities to A33 (K(d) of 0.14 nM to 20 nM) and may recognize the same epitope, which was determined to be conformational and located within amino acid residues 99 to 185 at the C terminus of A33. One or more of the MAbs were shown to reduce the spread of vaccinia virus as well as variola virus (the causative agent of smallpox) in vitro and to more effectively protect mice when administered before or 2 days after intranasal challenge with virulent vaccinia virus than a previously isolated mouse anti-A33 MAb (1G10) or vaccinia virus immunoglobulin. The protective efficacy afforded by anti-A33 MAb was comparable to that of a previously isolated chimpanzee/human anti-B5 MAb. The combination of anti-A33 MAb and anti-B5 MAb did not synergize the protective efficacy. These chimpanzee/human anti-A33 MAbs may be useful in the prevention and treatment of vaccinia virus-induced complications of vaccination against smallpox and may also be effective in the immunoprophylaxis and immunotherapy of smallpox and other orthopoxvirus diseases.