gebhart/patrepeval huggingface dataset:
Non-invasive measurement of human endothelium dependent arterial responses: accuracy and reproducibility. (1995)

Vascular responses to endothelium dependent and independent stimuli in systemic arteries can be studied non-invasively in man. Subjects should be studied on at least two occasions before and after any intervention, to optimise the chance of showing a significant effect from any potentially beneficial therapy.
Electrogenerated chemiluminescence. VII. Influence of an external magnetic field on luminescence intensity (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrogenerated chemiluminescence. VII. Influence of an external magnetic field on luminescence intensityLarry R. Faulkner, Hiroyasu Tachikawa, and Allen J. BardCite this: J. Am. Chem. Soc. 1972, 94, 3, 691–699Publication Date (Print):February 1, 1972Publication History Published online1 May 2002Published inissue 1 February 1972https://pubs.acs.org/doi/10.1021/ja00758a001https://doi.org/10.1021/ja00758a001research-articleACS PublicationsRequest reuse permissionsArticle Views775Altmetric-Citations174LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Numerical Convergence of the Landau‐Lifshitz Equations and Its Simulation (2010)

A difference scheme of Landau‐Lifshitz (LL for short) equations is studied. Their convergence and stability are proved. Furthermore, a new solution of LL equation is given for testing our scheme. At the end, three subcases of this LL equation are concerned about, and some properties about these equations are shown by a numeric simulation way.
Post-progression therapy (PPT) effect on survival in AVF2107, a phase III trial of bevacizumab in first-line treatment of metastatic colorectal cancer (mCRC) (2004)

3517 Background: Bevacizumab [Avastin (BV)], a recombinant humanized monoclonal antibody against VEGF, significantly prolongs overall survival (OS) when added to 1st-line chemotherapy for mCRC. BV joins 5-Fluorouracil/ leucovorin (5-FU/LV), irinotecan,(I) and oxaliplatin (Ox) as agents with demonstrated survival benefit in the treatment of mCRC. A recent analysis of phase III trials in mCRC, where treatment with 5-FU/LV, I, and Ox was available, demonstrates a correlation between the percentage of patients receiving all 3 active agents and OS (Grothey, J Clin Oncol, in press). We analyzed the effect of use of active agents over the treatment course on OS in AVF2107. Methods: The findings of AVF2107 have been reported. In AVF2107, use and composition of PPT (Ox-containing vs non-Ox-containing) was at the discretion of the investigator. OS was determined in the two randomized treatment arms [IFL/ placebo (Arm 1, n=412) and IFL/ BV (Arm 2, n=402)] according to PPT use and composition. Baseline characteristics were compared between these subgroups. Results: 231 (56.2%) and 222 (55.2%) patients received PPT in Arms 1 and 2, respectively. Table 1 summarizes OS by PPT composition. For Arm 1 patients who received PPT, the group receiving Ox PPT was younger (median age 57 vs 64 yrs), had better performance status (ECOG 0, 64 vs 52%) and a longer 1st-line PFS (7.4 vs 5.8 months) compared to those receiving non-Ox PPT. For Arm 2, differences between Ox and non-Ox PPT subgroups were not observed. Conclusion: BV prolongs survival in patients with mCRC when added to first-line chemotherapy. In addition, this subset analysis suggests that a treatment strategy incorporating all active agents over the course of disease optimizes OS. These results are consistent with an analysis of other recent phase III trials in mCRC. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Genentech, Inc. Eli Lilly & Co.; Genentech, Inc.
A Phylogenetic Analysis ofSaccharomyces Species by the Sequence of 18S–28S rRNA Spacer Regions (1997)

Sequences of two internally transcribed spacer regions between 18S and 28S rRNA genes were determined to assess the phylogenetic relationship in the strains belonging to the genus Saccharomyces. The sequences of S. bayanus and S. pastorianus were quite similar, but not identical. Two phylogenetic trees constructed by the neighbor-joining method showed that all the species examined were distinguished from one another. The Saccharomyces sensu stricto species: S. cerevisiae, S. bayanus, S. paradoxus and S. pastorianus, were closely related and far from the Saccharomyces sensu lato species including S. barnetti, S. castellii, S. dairensis, S. exiguus, S. servazzii, S. spencerorum and S. unisporus, and an outlying species, S. kluyveri.
Traumatic brain injury (2003)

Some issues in traumatic brain injury have now been resolved, and specific recommendations can be made. Fluid therapy directed toward a euvolemic state is now universally recommended, for example, as is the role of intracranial pressure monitoring. Other areas, such as the use of hypertonic saline, remain controversial. In both cases the authors have made an effort to cite the most recent literature, so that readers can draw their own conclusions from the original source material.
Liposomal Nanoparticles of a Spleen Tyrosine Kinase P-Site Inhibitor Amplify the Potency of Low Dose Total Body Irradiation Against Aggressive B-Precursor Leukemia and Yield Superior Survival Outcomes in Mice (2015)

This study was designed to improve the efficacy of radiation therapy against radiation-resistant leukemia. We report that the potency of low dose radiation therapy against B-precursor acute lymphoblastic leukemia (BPL) can be markedly enhanced by combining radiation with a liposomal nanoparticle (LNP) formulation of the SYK-P-site inhibitor C61 ("C61-LNP"). C61-LNP plus low dose total body irradiation (TBI) was substantially more effective than TBI alone or C61-LNP alone in improving the event-free survival outcome NOD/SCID mice challenged with an otherwise invariably fatal dose of human ALL xenograft cells derived from relapsed BPL patients. C61-LNP plus low dose TBI also yielded progression-free survival, tumor-free survival and overall survival outcomes in CD22ΔE12 × BCR-ABL double transgenic mice with advanced stage, radiation-resistant BPL with lymphomatous features that were significantly superior to those of mice treated with TBI alone or C61-LNP alone.
Preparation of sintered degenerate n-type PbTe with a small grain size and its thermoelectric properties (2002)

Sintered degenerate n-type PbTe samples with small grain sizes ranging from 0.7 to 4 μm were prepared and the effects of grain size on their thermoelectric properties were then investigated. The Seebeck coefficient of the sintered samples increased almost double when the grain size decreased from 4 to 0.7 μm. On the other hand, their electrical and thermal conductivity decreased with decreasing grain size. Accordingly, decreasing their grain size increased their thermoelectric figure-of-merit. A maximum value of the figure-of-merit of the obtained small grain-size samples was significantly higher than that of large grain-size samples with the same carrier concentration reported. This favorable result was caused mainly by the increase in the Seebeck coefficient. The influences of grain boundaries on the increase in the Seebeck coefficient were discussed. It is concluded that the Seebeck coefficient was increased by potential barrier scattering, which occurred at the grain boundaries in the sintered samples.
Rapid growth of a hepatocellular carcinoma and the driving mutations revealed by cell-population genetic analysis of whole-genome data (2011)

We present the analysis of the evolution of tumors in a case of hepatocellular carcinoma. This case is particularly informative about cancer growth dynamics and the underlying driving mutations. We sampled nine different sections from three tumors and seven more sections from the adjacent nontumor tissues. Selected sections were subjected to exon as well as whole-genome sequencing. Putative somatic mutations were then individually validated across all 9 tumor and 7 nontumor sections. Among the mutations validated, 24 were amino acid changes; in addition, 22 large indels/copy number variants (>1 Mb) were detected. These somatic mutations define four evolutionary lineages among tumor cells. Separate evolution and expansion of these lineages were recent and rapid, each apparently having only one lineage-specific protein-coding mutation. Hence, by using a cell-population genetic definition, this approach identified three coding changes (CCNG1, P62, and an indel/fusion gene) as tumor driver mutations. These three mutations, affecting cell cycle control and apoptosis, are functionally distinct from mutations that accumulated earlier, many of which are involved in inflammation/immunity or cell anchoring. These distinct functions of mutations at different stages may reflect the genetic interactions underlying tumor growth.
Acyclic analogs of lipid A: synthesis and biological activities. (1992)

The synthesis of a series of novel acyclic analogues of lipid A, the lipophilic terminal of lipopolysaccharide (LPS), is reported. In these compounds, the reducing glucose unit of lipid A has been replaced by an acyclic analogue unit (abbreviated as AAU) consisting of a spacer (of varying length), an (R)-3-hydroxytetradecanamido moiety (of varying configuration at the carbon of attachment), and a CO2H group. The AAU has been attached to the anomeric carbon of the nonreducing glucose unit of lipid A, either through glycosidic linkage or through an acyl linkage. Further, amide isosteres of these acyclic analogues have been prepared using suitably protected 2,3-diamino-2,3-dideoxyglucose instead of 2-amino-2-deoxyglucose. All the compounds were well characterized and were tested for their ability to induce TNF-alpha in mouse bone marrow-derived macrophages, to enhance nonspecific resistance to infection in mice and to induce endotoxic shock in mice. The results showed a dramatic dependence, for the first time, on the length of the spacer and on the configuration of the carbon bearing the amido group in the AAU part of the analogues.
Suppression of canonical Wnt/ -catenin signaling by nuclear plakoglobin recapitulates phenotype of arrhythmogenic right ventricular cardiomyopathy (2006)

Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVC) is a genetic disease caused by mutations in desmosomal proteins. The phenotypic hallmark of ARVC is fibroadipocytic replacement of cardiac myocytes, which is a unique phenotype with a yet-to-be-defined molecular mechanism. We established atrial myocyte cell lines expressing siRNA against desmoplakin (DP), responsible for human ARVC. We show suppression of DP expression leads to nuclear localization of the desmosomal protein plakoglobin and a 2-fold reduction in canonical Wnt/beta-catenin signaling through Tcf/Lef1 transcription factors. The ensuing phenotype is increased expression of adipogenic and fibrogenic genes and accumulation of fat droplets. We further show that cardiac-restricted deletion of Dsp, encoding DP, impairs cardiac morphogenesis and leads to high embryonic lethality in the homozygous state. Heterozygous DP-deficient mice exhibited excess adipocytes and fibrosis in the myocardium, increased myocyte apoptosis, cardiac dysfunction, and ventricular arrhythmias, thus recapitulating the phenotype of human ARVC. We believe our results provide for a novel molecular mechanism for the pathogenesis of ARVC and establish cardiac-restricted DP-deficient mice as a model for human ARVC. These findings could provide for the opportunity to identify new diagnostic markers and therapeutic targets in patients with ARVC.
Dimethylfumarate Inhibits TNF-Induced Nuclear Entry of NF-κB/p65 in Human Endothelial Cells (2002)

Fumaric acid esters, mainly dimethylfumarate (DMF), have been successfully used to treat psoriasis. Based on previous observations that DMF inhibited expression of several TNF-induced genes in endothelial cells, we wished to explore the molecular basis of DMF function in greater detail. In first experiments we analyzed DMF effects on tissue factor expression in human endothelial cells in culture, because tissue factor is expressed by two independent sets of transcription factors, by NF-kappa B via TNF and by early gene response-1 transcription factor via vascular endothelial growth factor (VEGF). We show that DMF inhibits TNF-induced tissue factor mRNA and protein expression as well as TNF-induced DNA binding of NF-kappa B proteins, but not VEGF-induced tissue factor protein, mRNA expression, or VEGF-induced early gene response-1 transcription factor/DNA binding. To determine where DMF interferes with the TNF/NF-kappa B signaling cascade, we next analyzed DMF effects on I kappa B and on the subcellular distribution of NF-kappa B. DMF does not inhibit TNF-induced I kappa B alpha phosphorylation and I kappa B degradation; thus, NF-kappa B is properly released from I kappa B complexes even in the presence of DMF. Importantly, DMF inhibits the TNF-induced nuclear entry of NF-kappa B proteins, and this effect appears selective for NF-kappa B after the release from I kappa B, because the constitutive shuttling of inactive NF-kappa B/I kappa B complexes into and out from the nucleus is not blocked by DMF. Moreover, DMF does not block NF-kappa B/DNA binding. In conclusion, DMF appears to selectively prevent the nuclear entry of activated NF-kappa B, and this may be the basis of its beneficial effect in psoriasis.
Differential Ability to Form the G Protein βγ Complex among Members of the β and γ Subunit Families (1996)

We have determined the relative abilities of several members of the G protein beta and gamma subunit families to associate with each other using the yeast two-hybrid system. We show first that the mammalian beta1 and gamma3 fusion proteins form a complex in yeast and that formation of the complex activates the reporter gene for beta-galactosidase. Second, the magnitude of reporter activity stimulated by various combinations of beta and gamma subunit types varies widely. Third, the reporter activity evoked by a particular combination of beta and gamma subunit types is not correlated with the expression levels of these subunit types in the yeast cells. Finally, the reporter activity shows a direct relationship with the amount of hybrid betagamma complex formed in the cell as determined by immunoprecipitation. These results suggest that different beta and gamma subunit types interact with each other with widely varying abilities, and this in combination with the level of expression of a subunit type in a mammalian cell determines which G protein will be active in that cell. The strong preference of all gamma subunit types for the beta1 subunit type explains the preponderence of this subunit type in most G proteins.
Diet and Parkinson's disease: A potential role of dairy products in men (2002)

Diet may play a causative role in Parkinson's disease (PD), but potential associations between diet and PD risk rarely have been assessed in prospective studies. We investigated associations between food intakes and PD risk in two large prospective cohorts in which 210 incident PD cases in men and 184 in women were documented. A positive association was found between dairy intake and PD risk in men (relative risk [RR] comparing extreme categories, 1.8; p trend = 0.004), but not in women (RR, 1.1; p trend = 0.9). No other food groups were associated with PD risk in either men or women. Further analyses among men showed significant positive associations with PD risk for intakes of several dairy foods as well as dairy calcium (RR, 1.5; p trend = 0.02), dairy vitamin D (RR, 1.6; p trend = 0.004), dairy protein (RR, 1.6; p trend = 0.01), and lactose (RR, 1.8; p trend = 0.002), but not dairy fat (RR, 1.1; p trend = 0.4). Intakes of calcium, vitamin D, and protein from other dietary or supplemental sources were not related to PD risk in men. Our results suggest that higher intake of dairy products may increase the risk of PD in men; however, this finding needs further evaluation, and the underlying active components need to be identified.
The mutual effects between orthodontic tooth movement and estrous cycle or estrogen 1 (2009)

The mutual effects between orthodontic tooth movement and estrous cycle or estrogen of female rats were studied. Animals were randomly divided into control group, force-loading for one time group, force-loading for five times group and sham groups. Each group included four subgroups, based on the estrous cycle stage. Serum estradiol, the change of estrous cycle, and tooth movement distance were measured. Significant variations in the estradiol level were found among the groups receiving force during the same stage of the estrous cycle (p < 0.01) and among the subgroups within each group (p < 0.01). The experimental treatments affected the estrous cycle of the groups receiving force in metestrus and diestrus. There were significant variations in the amount of tooth movement among the groups receiving force during different stages of the estrous cycle (p < 0.01). The largest amount of tooth movement was in the estrus group. The results show it was more desirable to wear appliances and apply force during estrus.
CHO cells knocked out for TSC2 display an improved productivity of antibodies under fed batch conditions (2016)

ABSTRACT The kinase mTOR operates in two cellular complexes, mTORC1 and mTORC2. mTORC1 adjusts metabolic activity according to external growth conditions and nutrients availability. When conditions are prosperous, mTOR facilitates protein and lipid biosyntheses and inhibits autophagy, while under metabolic constraints, however, its attenuation induces a catabolic program, energy preservation and autophagy. CHO is a key cell line for manufacturing of biologics owing to its remarkable ability to grow to high densities and maintain protein production and secretion for extended times. While high mTOR activity has been associated with high productivity in CHO cells, its inhibition by rapamycin has also been documented to augment productivity via promotion of viability. Here using CRISPR/Cas9 editing we engineered CHO cells to enforce high mTORC1 activity by knocking‐out TSC2, a major mTOR inhibitory protein, or PTEN, a phosphatase that attenuates the PI3K/AKT/mTOR pathway. Only TSC2‐deleted cells exhibited a constitutive activation of mTORC1 under fed batch conditions. Cells grew larger in size, synthesized more proteins and displayed an over twofold elevation in their specific productivity. While peak viable cell density was compromised, overall titers increased to an extent dependent upon the parental clone. Our data underscore manipulation of TSC as a strategy to improve performance of CHO cell in bioreactors. Biotechnol. Bioeng. 2016;113: 1942–1952. © 2016 Wiley Periodicals, Inc.
Cyclic oligomers of poly(ether ketone) (PEK): synthesis, extraction from polymer, fractionation, and characterisation of the cyclic trimer, tetramer and pentamer (2000)

Exhaustive extraction of poly(ether ketone) (PEK) with hot chloroform affords a novel range of cyclic oligo(ether ketone)s. The proportion of cyclic material extracted in this way ranged from as high as 13% for laboratory-synthesised PEK to as low as 2% for an industrial development-grade polymer. Extracts were characterised by 1H NMR spectroscopy, GPC, and MALDI-TOF mass spectrometry, which indicated the presence of ring sizes from the cyclic trimer up to at least the cyclic decamer. These macrocyclic oligomers were also prepared (in ca. 80% total yield) by self-polycondensation of 4-fluoro-4′-hydroxybenzophenone under pseudo-high-dilution conditions. Column chromatography on silica gel enabled isolation of the cyclic trimer, cyclic tetramer and cyclic pentamer as pure compounds, characterised by NMR spectroscopy, mass spectrometry, elemental analysis and (for the cyclic trimer and cyclic tetramer) X-ray crystallography. Ring-opening polymerisation of mixed cyclic oligomers was achieved in the melt at 385 °C, using the potassium salt of 4-hydroxybenzophenone as initiator.
Cyclopropanoic fatty acids of litchi (Litchi chinensis) seed oil. A reinvestigation (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCyclopropanoic fatty acids of litchi (Litchi chinensis) seed oil. A reinvestigationEmile M. Gaydou, Auguste. Ralaimanarivo, and Jean Pierre. BianchiniCite this: J. Agric. Food Chem. 1993, 41, 6, 886–890Publication Date (Print):June 1, 1993Publication History Published online1 May 2002Published inissue 1 June 1993https://pubs.acs.org/doi/10.1021/jf00030a009https://doi.org/10.1021/jf00030a009research-articleACS PublicationsRequest reuse permissionsArticle Views331Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts