gebhart/patrepeval huggingface dataset:
A small-molecule inhibitor of Tcf/β-catenin signaling down-regulates PPARγ and PPARδ activities (2008)

Activation of the Wnt/beta-catenin signaling pathway occurs in several types of cancers and thus it is an attractive target for anticancer drug development. To identify compounds that inhibit this pathway, we screened a chemical library using a cell-based beta-catenin/Tcf-responsive reporter. We identified FH535, a compound that suppresses both Wnt/beta-catenin and peroxisome proliferator-activated receptor (PPAR) signaling. FH535 antagonizes both PPARgamma and PPARdelta ligand-dependent activation and shows structural similarity to GW9662, a known PPARgamma antagonist. The effect of FH535 on beta-catenin/Tcf activity is reduced in cells carrying a deletion of the PPARdelta gene, as well as by the PPARgamma agonist lysophosphatidic acid. Mechanistically, FH535 inhibits recruitment of the coactivators beta-catenin and GRIP1 but not the corepressors NCoR and SMRT. Its repression of beta-catenin recruitment, in comparison with GW9662, is linked to FH535's unique capability to inhibit the Wnt/beta-catenin signaling pathway. The antiproliferation effect of the compound observed on some transformed colon lung and liver cell lines is suggestive of its potential therapeutic value in the treatment of cancer.
Two Effective Seminars by Medical Management Development Associates, Inc. (1984)

1A Guide to Managing Hospital Utilization Effectively 2DRG Based Discharge Planning presented by Lyman E. Martin
Extracellular matrix signatures of human mammary carcinoma identify novel metastasis promoters (2014)

The extracellular matrix (ECM) is a major component of tumors and a significant contributor to cancer progression. In this study, we use proteomics to investigate the ECM of human mammary carcinoma xenografts and show that primary tumors of differing metastatic potential differ in ECM composition. Both tumor cells and stromal cells contribute to the tumor matrix and tumors of differing metastatic ability differ in both tumor- and stroma-derived ECM components. We define ECM signatures of poorly and highly metastatic mammary carcinomas and these signatures reveal up-regulation of signaling pathways including TGFβ and VEGF. We further demonstrate that several proteins characteristic of highly metastatic tumors (LTBP3, SNED1, EGLN1, and S100A2) play causal roles in metastasis, albeit at different steps. Finally we show that high expression of LTBP3 and SNED1 correlates with poor outcome for ER(-)/PR(-)breast cancer patients. This study thus identifies novel biomarkers that may serve as prognostic and diagnostic tools. DOI: http://dx.doi.org/10.7554/eLife.01308.001.
Lipoprotein Receptors in the Nervous System (2002)

The low-density-lipoprotein (LDL) receptor family is an evolutionarily ancient gene family of structurally closely related cell-surface receptors. Members of the family are involved in the cellular uptake of extracellular ligands and regulate diverse biological processes including lipid and vitamin metabolism and cell-surface protease activity. Some members of the family also participate in cellular signaling and regulate the development and functional maintenance of the nervous system. Here we review the roles of this family of multifunctional receptors in the nervous system and focus on recent advances toward the understanding of the mechanisms by which lipoprotein receptors and their ligands transmit and modulate signals in the brain.
Modeling and Control of Aggregated Heterogeneous Thermostatically Controlled Loads for Ancillary Services (2011)

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The anti-ischemic drugs defibrotide and oligotide analogously inhibit leukocyte-endothelial cell adhesion in vitro (1996)

Abstract Defibrotide (a polydeox-yribonucleotide) and oligotide (an oligodeoxyribonucleotide) obtained from mammalian single-stranded DNA, have been demonstrated to have anti-ischemic activity in some experimental models of ischemia/ reperfusion of kidney in rats. We hypothesized that their anti-is chemic activity could be related to an inhibition of leukocyte-endothe lial cell adhesion and also the con sequent generation of oxygen free radicals by leukocytes. We studied the in vitro adhesion of neutrophils to human umbilical vein endothelial cells under basal conditions and following neutrophil or endothelial cell activation (using 10--7 fMLP and SO0 U/ml TNF-α, respectively). De fibrotide and oligotide significantly inhibited neutrophil adhesion to endothelial cells (after only 1 min of drug treatment). When the anti-LFA-1 70H12 F(ab)2 monoclonal antibody was used, the drugs exerted only slight additional inhibition of the adhesion of fMLP-acti-vated neutrophils to endothelium. These results, confirmed in NIH/ 3T3–ICAM-l-transfected cells, demonstrate that defibrotide and oligo tide interfere with leukocyte adhesion to endothelial cells by an LFA-1–dependent mechanism.
Pivotal role of VASP in Arp2/3 complex–mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility (2001)

The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. In vitro, the actin monomer-binding region of ActA is critical for stimulating Arp2/3-dependent actin nucleation; however, this region is dispensable for actin-based motility in cells. Here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (VASP) recruitment by ActA can bypass defects in actin monomer-binding. Furthermore, purified VASP enhances the actin-nucleating activity of wild-type ActA and the Arp2/3 complex while also reducing the frequency of actin branch formation. These data suggest that ActA stimulates the Arp2/3 complex by both VASP-dependent and -independent mechanisms that generate distinct populations of actin filaments in the comet tails of L. monocytogenes. The ability of VASP to contribute to actin filament nucleation and to regulate actin filament architecture highlights the central role of VASP in actin-based motility.
Synthesis and Biological Evaluation of a Cytarabine Phosphoramidate Prodrug (2003)

Recently, we reported a novel approach for the intracellular delivery of the anti-cancer nucleotide 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP) using phosphoramidate-based prodrugs. These phosphoramidate prodrugs contain an ester group that undergoes intracellular activation, liberating phosphoramidate anion, which in turn undergoes spontaneous cyclization and P-N bond cleavage to yield the nucleoside monophosphate quantitatively. This approach has now been extended to cytarabine [1-beta-D-arabinofuranosylcytosine (Ara-C)], an anti-cancer nucleoside that is limited in its utility because of poor intracellular transport characteristics and weak activity as a substrate for tumor cell kinases. The cytarabine phosphoramidate prodrug 1 has been synthesized and evaluated in comparison with cytarabine for growth inhibitory activity against wild-type, nucleoside transport-deficient, and nucleoside kinase-deficient CEM leukemia cell lines. The prodrug was comparable in growth inhibitory activity (IC50 = 32 nM) to cytarabine (IC50 = 16 nM) in wild-type CCRF-CEM cells following drug treatment for 72 h. The nucleoside transport-deficient CEM/AraC8C exhibited a high level of resistance (6400-fold) to cytarabine but was more sensitive (210-fold resistant vs CCRF-CEM cells) to prodrug 1. Similarly, the deoxycytidine kinase-deficient cell line (CEM/dCK-) was highly resistant to cytarabine (13900-fold) but more sensitive (106-fold resistant vs CCRF-CEM cells) to prodrug 1. These results indicate that prodrug 1 is significantly more potent than cytarabine against transport- and kinase-deficient cell lines and are consistent with a mechanism involving intracellular delivery of cytarabine 5'-monophosphate.
Patient-Controlled Methylphenidate for the Management of Fatigue in Patients With Advanced Cancer: A Preliminary Report (2003)

These preliminary results suggest that patient-controlled methylphenidate administration rapidly improved fatigue and other symptoms. Randomized controlled trials are justified.
Could battery-less scatter radio tags achieve 270-meter range? (2016)

This paper studies whether increased ranges of bistatic scatter radio communication are possible, especially when low-cost, embedded receivers, originally designed for conventional radio (and not for scatter radio) are employed. Wireless power transmission and bistatic scatter radio are closely related, and thus, this work aims to highlight a new exciting, potentially interesting, key-enabling research direction. It is found that for 13 dBm emitter transmission power, 246 meters scatter radio tag-to-reader distance is possible, with packet error rate (PER) less than 1%, while 268 meters are possible at the expense of increased PER, in the order of 10%.
Isolation of a Cytopathogenic Agent from Human Adenoids Undergoing Spontaneous Degeneration in Tissue Culture (1953)

Summary1. From the present evidence it appears that an unidentified, possibly new, tissue culture cytopathogenic agent has been isolated repeatedly from human adenoids undergoing spontaneous degeneration in tissue culture. The filter ability and the inability to cultivate the agent on bacteriological media and to demonstrate organisms in stained tissue culture preparations would indicate that the agent belongs to the group of viruses or rickettsial It is tentatively proposed to designate the agent as the “adenoid degeneration agent”, abbreviated as “A.D. agent”. 2. That the agent is derived from the adenoid tissue rather than from the nutrient media is indicated by the fact that some adenoids and all human embryonic tissues cultivated in the identical media and at the same time have not undergone degeneration, although they are susceptible to infection with the agent; also, repeated attempts to isolate the agents from adenoid cultures not demonstrating degeneration have been uniformly unsuccessful. 3. Further investigation is in progress to determine the relation of the agent to the adenoids and to study their possible role in human disease; particularly upper respiratory infections.
Alternative Therapies for Children with Brain Stem Gliomas: Immunotherapy and Gene Therapy (1996)

Alternative means are needed for the treatment of childhood brain stem gliomas. Both immunotherapy and gene therapy have and will be, in the future, evaluated for these patients. Results, to date, with immunotherapy have been sparse and variable. Restorative immunotherapy, especially the use of beta-interferon, has shown some promising results. However, in one recently completed trial, the combination of beta-interferon plus hyperfractionated radiotherapy in newly diagnosed patients did not apparently result in improved survival. Gene therapy, still in its infancy, also may be potentially applicable to the treatment of children with brain stem gliomas.
Pharmacology of Cannabinoid Receptor Ligands (1999)

Mammalian tissues contain at least two types of cannabinoid receptor, CB&lt;sub&gt;1&lt;/sub&gt; and CB&lt;sub&gt;2&lt;/sub&gt; , both coupled to G proteins. CB&lt;sub&gt;1&lt;/sub&gt; receptors are expressed mainly by neurones of the central and peripheral nervous system whereas CB&lt;sub&gt;2&lt;/sub&gt; receptors occur in certain non-neuronal tissues, particularly in immune cells. The existence of endogenous ligands for cannabinoid receptors has also been demonstrated. The discovery of this 'endogenous cannabinoid system' has been paralleled by a renewed interest in possible therapeutic applications of cannabinoids, for example in the management of pain and in the suppression of muscle spasticity/spasm associated with multiple sclerosis or spinal cord injury. It has also prompted the development of a range of novel cannabinoid receptor ligands, including several that show marked selectivity for CB&lt;sub&gt;1&lt;/sub&gt; or CB&lt;sub&gt;2&lt;/sub&gt; receptors. This review summarizes current knowledge about the in vitro pharmacological properties of important CB&lt;sub&gt;1&lt;/sub&gt; and CB&lt;sub&gt;2&lt;/sub&gt; receptor ligands. Particular attention is paid to the binding properties of these ligands, to the efficacies of cannabinoid receptor agonists, as determined using cyclic AMP or [&lt;sup&gt;35&lt;/sup&gt;S]GTP&amp;#947;S binding assays, and to selected examples of how these pharmacological properties can be influenced by chemical structure. The in vitro pharmacological properties of ligands that can potently and selectively oppose the actions of CB&lt;sub&gt;1&lt;/sub&gt; or CB&lt;sub&gt;2&lt;/sub&gt; receptor agonists are also described. When administered by themselves, some of these ligands produce effects in certain tissue preparations that are opposite in direction to those produced by cannabinoid receptor agonists and the possibility that the ligands producing such 'inverse cannabimimetic effects' are inverse agonists rather than pure antagonists is discussed.
An evaluation of alternative architectures for transaction processing in the cloud (2010)

Cloud computing promises a number of advantages for the deployment of data-intensive applications. One important promise is reduced cost with a pay-as-you-go business model. Another promise is (virtually) unlimited throughput by adding servers if the workload increases. This paper lists alternative architectures to effect cloud computing for database applications and reports on the results of a comprehensive evaluation of existing commercial cloud services that have adopted these architectures. The focus of this work is on transaction processing (i.e., read and update workloads), rather than analytics or OLAP workloads, which have recently gained a great deal of attention. The results are surprising in several ways. Most importantly, it seems that all major vendors have adopted a different architecture for their cloud services. As a result, the cost and performance of the services vary significantly depending on the workload.
Regulation of nuclear translocation of Forkhead transcription factor AFX by protein kinase B (1999)

The regulation of intracellular localization of AFX, a human Forkhead transcription factor, was studied. AFX was recovered as a phosphoprotein from transfected COS-7 cells growing in the presence of FBS, and the phosphorylation was eliminated by wortmannin, a potent inhibitor of phosphatidylinositol (PI) 3-kinase. AFX was phosphorylated in vitro by protein kinase B (PKB), a downstream target of PI 3-kinase, but a mutant protein in which three putative phosphorylation sites of PKB had been replaced by Ala was not recognized by PKB. In Chinese hamster ovary cells (CHO-K1) cultured with serum, the AFX protein fused with green fluorescence protein (AFX-GFP) is localized mainly in the cytoplasm, and wortmannin induced transient nuclear translocation of the fusion protein. The AFX-GFP mutant in which all three phosphorylation sites had been replaced by Ala was detected exclusively in the cell nucleus. AFX-GFP was in the nucleus when the cells were infected with an adenovirus vector encoding a dominant-negative form of either PI 3-kinase or PKB, whereas the fusion protein stayed in the cytoplasm when the cells expressed constitutively active PKB. In CHO-K1 cells expressing AFX-GFP, DNA fragmentation was induced by the stable PI 3-kinase inhibitor LY294002, and the expression of the active form of PKB suppressed this DNA fragmentation. The phosphorylation site mutant of AFX-GFP enhanced DNA fragmentation irrespective of the presence and absence of PI 3-kinase inhibitor. These results indicate that the nuclear translocation of AFX is negatively regulated through its phosphorylation by PKB.
Inhibition of Listeria monocytogenes by fatty acids and monoglycerides (1992)

Fatty acids and monoglycerides were evaluated in brain heart infusion broth and in milk for antimicrobial activity against the Scott A strain of Listeria monocytogenes. C12:0, C18:3, and glyceryl monolaurate (monolaurin) had the strongest activity in brain heart infusion broth and were bactericidal at 10 to 20 micrograms/ml, whereas potassium (K)-conjugated linoleic acids and C18:2 were bactericidal at 50 to 200 micrograms/ml. C14:0, C16:0, C18:0, C18:1, glyceryl monomyristate, and glyceryl monopalmitate were not inhibitory at 200 micrograms/ml. The bactericidal activity in brain heart infusion broth was higher at pH 5 than at pH 6. In whole milk and skim milk, K-conjugated linoleic acid was bacteriostatic and prolonged the lag phase especially at 4 degrees C. Monolaurin inactivated L. monocytogenes in skim milk at 4 degrees C, but was less inhibitory at 23 degrees C. Monolaurin did not inhibit L. monocytogenes in whole milk because of the higher fat content. Other fatty acids tested were not effective in whole or skim milk. Our results suggest that K-conjugated linoleic acids or monolaurin could be used as an inhibitory agent against L. monocytogenes in dairy foods.
Opportunities and Challenges in Materials Development for Thin Film Solid Oxide Fuel Cells (2008)

Abstract Solid oxide fuel cells (SOFCs) are currently the focus of intense investigation given their high chemical‐to‐electrical energy conversion efficiency and low carbon footprint. In this review, the development of thin film SOFCs, sometimes described as ‘micro‐SOFCs', is highlighted and analysed. Opportunities for reduced temperature operation and portable power generation arise from the decreased thickness of the solid electrolyte, as well at the metastable phases and nanoscale‐dependent effects that are a consequence of the reduced temperature of fabrication. Challenges such as enhanced cation diffusion along grain boundaries are; however, also observed, potentially impacting the long‐term stability of these devices. Recent progress achieved in understanding these and other challenges are reviewed and directions for future work identified.
Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.) (1995)

Sink strength of growing potato tubers is believed to be limited by sucrose metabolism and/or starch synthesis. Sucrose synthase (Susy) is most likely responsible for the entire sucrose cleavage in sink tubers, rather than invertases. To investigate the unique role of sucrose synthase with respect to sucrose metabolism and sink strength in growing potato tubers, transgenic potato plants were created expressing Susy antisense RNA corresponding to the T-type sucrose synthase isoform. Although the constitutive 35S CaMV promoter was used to drive the expression of the antisense RNA the inhibition of Susy activity was tuber-specific, indicating that independent Susy isoforms are responsible for Susy activity in different potato organs. The inhibition of Susy leads to no change in sucrose content, a strong accumulation of reducing sugars and an inhibition of starch accumulation in developing potato tubers. The increase in hexoses is paralleled by a 40-fold increase in invertase activities but no considerable changes in hexokinase activities. The reduction in starch accumulation is not due to an inhibition of the major starch biosynthetic enzymes. The changes in carbohydrate accumulation are accompanied by a decrease in total tuber dry weight and a reduction of soluble tuber proteins. The reduced protein accumulation is mainly due to a decrease in the major storage proteins patatin, the 22 kDa proteins and the proteinase inhibitors. The lowered accumulation of storage proteins is not a consequence of the availability of the free amino acid pool in potato tubers. Altogether these data are in agreement with the assumption that sucrose synthase is the major determinant of potato tuber sink strength. Contradictory to the hypothesis that the sink strength of growing potato tubers is inversely correlated with the tuber number per plant, no increase in tuber number per plant was found in Susy antisense plants.
Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell (2003)

Our study suggests that the non-cytotoxic concentrations of docetaxel have strong antiangiogenic activity on LS174T Cells, which suggests docetaxel may be a promising antiangiogenic agent.
Designing pixel-oriented visualization techniques: theory and applications (2000)

Visualization techniques are of increasing importance in exploring and analyzing large amounts of multidimensional information. One important class of visualization techniques which is particularly interesting for visualizing very large multidimensional data sets is the class of pixel-oriented techniques. The basic idea of pixel-oriented visualization techniques is to represent as many data objects as possible on the screen at the same time by mapping each data value to a pixel of the screen and arranging the pixels adequately. A number of different pixel-oriented visualization techniques have been proposed in recent years and it has been shown that the techniques are useful for visual data exploration in a number of different application contexts. In this paper, we discuss a number of issues which are important in developing pixel-oriented visualization techniques. The major goal of this article is to provide a formal basis of pixel-oriented visualization techniques and show that the design decisions in developing them can be seen as solutions of well-defined optimization problems. This is true for the mapping of the data values to colors, the arrangement of pixels inside the subwindows, the shape of the subwindows, and the ordering of the dimension subwindows. The paper also discusses the design issues of special variants of pixel-oriented techniques for visualizing large spatial data sets.
Manipulation of a single cell with microcapillary tubing based on its electrophoretic mobility (1995)

Manipulation of a single cell of spherical shape, approximately 5-10 microns in diameter, was performed with capillary tubing and an electrostatic field. A single cell migrates with its electrophoretic mobility into capillary tubing against the flow of electroosmosis coming out of the capillary. After trapping the cell in the capillary, it is pulled out into the other microreservoir with the application of a reverse electric voltage. When we apply a negative voltage to the microreservoir itself, the cell in it can keep floating for a relatively long period due to electrostatic repulsion. The electrophoretic mobility of a single cell is also estimated.
Lane detection using smoothing spline (2010)

In vehicle safety research area, a Lane Departure Warning System (LDWS) is of a major interest. The system, however, needs lane detection and lane tracking as basis. This paper proposes an algorithm used to detect the lane as an initialization for lane tracking. The algorithm takes an image captured from a video stream as an input. It then segments the image into a number of small tiles and applies the Principle Component Analysis (PCA) to each tile in order to find the centroid and the principle axis. After recovering the original image from all of the tiles, the probable lines are found using k-means clustering techniques. Two out of these lines will be selected to be the lanes of the road. At the end, the corresponding centroid points are chosen to be represented as control points of smoothing splines represented the lane. This obtained splines are aimed to be used further as an initialization of the lane tracking algorithm. The method has been tested and worked fine. However, its limitation has also been stated and needed to be improved in the future.