gebhart/patrepeval huggingface dataset:
IRON ABSORPTION FROM THE INTESTINAL TRACT (1962)

SUMMARY Groups of rats were injected with “Imferon” and with red cells to increase the storage iron. After seven weeks a tracer dose of iron‐59 was given to each animal and three weeks later the percentage of the dose in the blood, liver and spleen was determined by radioassay. Compared with control animals, decreased absorption of iron‐59 was found in the rats loaded with “Imferon” and with red cells. This suggests that the size of the iron stores is an important factor regulating iron absorption. Some of the unrecovered red cell iron was in tissues other than those assayed, but a large amount was probably lost from the body during the experimental period. These considerations also applied to the “Imferon” injected animals, but in addition some of the “Imferon” was still present in the mesenteric lymph tissues.
Electromechanical Control Systems and Devices (1977)

Electromechanical control systems and devices , Electromechanical control systems and devices , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Synthesis and Biological Activity of a Novel Series of 6-Substituted Thieno[2,3-d]pyrimidine Antifolate Inhibitors of Purine Biosynthesis with Selectivity for High Affinity Folate Receptors over the Reduced Folate Carrier and Proton-Coupled Folate Transporter for Cellular Entry† (2009)

A series of seven 2-amino-4-oxo-6-substituted thieno[2,3-d]pyrimidines with bridge length variations (from 2 to 8 carbon atoms) were synthesized as selective folate receptor (FR) alpha and beta substrates and as antitumor agents. The syntheses were accomplished from appropriate allylalcohols and 4-iodobenzoate to afford the aldehydes, which were converted to the appropriate 2-amino-4-carbethoxy-5-substituted thiophenes 23-29. Cyclization with chloroformamidine afforded the thieno[2,3-d]pyrimidines 30-36, which were hydrolyzed and coupled with diethyl-L-glutamate, followed by saponification, to give the target compounds 2-8. Compounds 3-6 were potent growth inhibitors (IC(50) 4.7-334 nM) of human tumor cells (KB and IGROV1) that express FRs. In addition, compounds 3-6 inhibited the growth of Chinese hamster ovary (CHO) cells that expressed FRs but not the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT). However, the compounds were inactive toward CHO cells that lacked FRs but contained either the RFC or PCFT. By nucleoside and 5-amino-4-imidazole carboxamide (AICA) protection studies, along with in vitro and in situ enzyme activity assays, the mechanism of antitumor activity was identified as the dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, AICA ribonucleotide formyltransferase. The dual inhibitory activity of the active thieno[2,3-d]pyrimidine antifolates and the FR specificity represent unique mechanistic features for these compounds distinct from all other known antifolates. The potent inhibitory effects of compounds 3-6 toward cells expressing FRs but not PCFT provide direct evidence that cellular uptake of this series of compounds by FRs does not depend on the presence of PCFT and argues that direct coupling between these transporters is not obligatory.
Insulin‐mediated sensitization of adenylyl cyclase activation (1993)

1. Insulin may be an important regulator of vascular function. We have previously studied lymphocyte beta-adrenoceptors as a model for the human vascular beta-adrenoceptor. To examine the effects of insulin on human beta-adrenoceptor responsiveness, adenylyl cyclase activity, cyclic AMP-dependent protein kinase activity and beta-adrenoceptor radioligand binding assays were performed on permeabilized mononuclear leukocytes. 2. With acute exposure to insulin in vitro, followed by washing and permeabilization there was a dose-dependent increase in both lymphocyte NaF-stimulated activity and beta-adrenoceptor-stimulated adenylyl cyclase activity paralleling an increase in beta-adrenoceptor-stimulated protein kinase A activity. Manganese-, forskolin- and forskolin plus guanylimidodiphosphate-stimulated adenylyl cyclase activities were not altered by insulin pretreatment. Additionally, mononuclear leukocyte beta-adrenoceptor density, proportion of externalized receptors and receptor affinity for agonist were not altered. 3. The data indicate that acute exposure to insulin sensitizes G-protein-stimulated adenylyl cyclase activity. These findings suggest a potential role for insulin in the regulation of beta-adrenoceptor responsiveness in man.
Tubulogenesis from isolated single cells of adult mammalian kidney: clonal analysis with a recombinant retrovirus (1996)

The adult mammalian kidney tubule epithelium exists in a relatively dormant, slowly replicative state but has a large potential for regenerative morphogenesis following severe ischemic or toxic injury. Under selective serum-free growth conditions, which included epidermal growth factor and retinoic acid, a subpopulation of renal proximal tubule cells isolated from adult rabbit kidney were grown in cell culture. These cells possessed two important characteristics: 1) an ability to differentiate morphogenically into tubule structures when grown in three-dimensional collagen gels and 2) a high capacity for self-renewal, since cell lineage analysis with a recombinant retrovirus demonstrated that in vitro tubulogenesis arose from clonal expansion of a single cell. Thus individual cells in the adult kidney have retained the ability for kidney tubulogenesis in vitro.
Antigenic modulation and receptor loss in experimental autoimmune myasthenia gravis (1979)

Immunization of groups of rats with 0.1- 100 microgram of acetylcholine receptor (AChR) purified from the electric organ of Torpedo californica resulted in dose-dependent (1) loss of acetylcholine receptor from the rats' muscles, (2) binding of antibodies to many of the receptors remaining in muscle and (3) production of antibodies in serum capable of cross-reacting with receptor solubilized from rat muscle. Addition of antibodies from rats immunized with electric organ acetylcholine receptors to muscle cells in culture caused loss of receptor by accelerating the rate of receptor degradation. Monovalent antibody fragments did not accelerate degradation unless antiantibody was added to cross-link the monovalent antibody fragments bound to receptors. This indicates that cross-linking of receptors by antibody molecules triggers accelerated receptor degradation, leading to receptor loss. The rate of increase in receptor destruction due to antigenic modulation observed in vitro appears sufficient to account for the extent of receptor loss observed in vivo. Endocytosis of antibody cross-linked receptors may be a rate-limiting step common to antigenic modulation in vitro and in vivo.
Synthetic amphipathic sequences of surfactant protein-B mimic several physicochemical and in vivo properties of native pulmonary surfactant proteins. (1992)

A mixture of lipids and proteins unique to the lung lines the airspaces of all mammalian species. This mixture, termed pulmonary surfactant, is essential for normal lung function. We have synthesized selected amino acid sequences of one of the major low molecular weight surfactant proteins to find whether these peptides can duplicate effects of native protein. Peptide/lipid mixtures approximate results found with native surfactant proteins both in vitro and in vivo. Effects found with native proteins or synthetic peptides include association with, and ordering of, surfactant lipid; changes in surface tension with surface compression; rapid adsorption of lipids from subphase to surface; and improvements in oxygenation of surfactant-deficient rats.
A High-Power Dual Six-Port Automatic Network Analyzer Used in Determining Biological Effects of Rf and Microwave Radiation (1981)

The design, calibration, and performance of a high-power (1-1000 W) automatic network analyzer based on the six-port concept are described for the 10-100-MHz range. Calibration is performed with a length of transmission line as the only impedence standard needed. A 10-mW thermistor mount is the standard of power. Imprecision in measnring reflection coefficient Gamma is 0.0001 in magnitude and 0.005/|Gamma| degrees in phase. Corresponding estimated systematic errors are 0.001 and 0.1/|Gamma| degrees. Imprecision in measuring power is 0.01 percent of range (20 W, 200 W, or 1000 W) with an estimated systematic error of 1.25 percent of reading.
Antitumor 2-(Aminocarbonyl)-1,2-bis(methylsulfonyl)-1-(2-chloroethyl)- hydrazines (1996)

Several 2-(aminocarbonyl)-1,2-bis(methylsulfonyl)-1-(2-chloroethyl)hydr azi nes were synthesized and primarily evaluated for antitumor activity against the murine L1210 leukemia. All of the compounds tested were capable of producing "cures" of mice bearing this tumor. One of the most active agents of this class, 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)- 2(-)[[2-chloroethyl)-amino]carbonyl]hydrazine, was further evaluated against a spectrum of transplanted murine and human solid tumors. Pronounced activity was found against all of the tumors including the murine B16F10 melanoma, M109 lung carcinoma, M5076 reticulum cell sarcoma, and the human LX-1 lung carcinoma. The activities observed compared favorably with those of the established antitumor drugs, cyclophosphamide, mitomycin C, and the nitrosoureas, evaluated concomitantly.
Regulation of the Human P-selectin Promoter by Bcl-3 and Specific Homodimeric Members of the NF-κB/Rel Family (1995)

P-selectin, an adhesion receptor for leukocytes, is constitutively expressed by megakaryocytes and endothelial cells. Synthesis of P-selectin is also increased by some inflammatory mediators. We characterized a previously identified kappa B site (-218GGGGGTGACCCC-207) in the promoter of the human P-selectin gene. The kappa B site was unique in that it bound constitutive nuclear protein complexes containing p50 or p52, but not inducible nuclear protein complexes containing p65. Furthermore, the element bound recombinant p50 or p52 homodimers, but not p65 homodimers. Methylation interference analysis indicated that p50 or p52 homodimers contacted the guanines at positions -218 to -214 on the coding strand and at -210 to -207 on the noncoding strand. Changes in the three central residues at -213 to -211 altered binding specificity for members of the NF-kappa B/Rel family. Mutations that eliminated binding to NF-kappa B/Rel proteins reduced by approximately 40% the expression of a reporter gene driven by the P-selectin promoter in transfected bovine aortic endothelial cells. Overexpression of p52 enhanced P-selectin promoter activity, and co-overexpression of Bcl-3 further induced promoter activity in a kappa B site-dependent manner. In contrast, overexpression of p50 repressed promoter activity; this repression was prevented by co-overexpression of Bcl-3. Similar phenomena were observed with reporter gene constructs driven by two tandem P-selectin kappa B sequences linked to the SV40 minimal promoter. These data suggest that Bcl-3 differentially regulates the effects of p50 and p52 homodimers bound to the kappa B site of the P-selectin promoter. This site may be a prototype for kappa B elements in other genes that bind specifically to p50 and/or p52 homodimers.
Electromagnetic Breast Imaging: Average Tissue Property Values in Women with Negative Clinical Findings (2004)

Representative data are provided for three electromagnetic breast imaging techniques-near-infrared spectroscopy, electrical impedance spectroscopy, and microwave imaging spectroscopy-to serve as potential benchmarks for future investigation. The breasts of 23 women without clinical or mammographic findings of disease were imaged in the coronal plane with nonionizing radiation of varying frequencies. Average electromagnetic property values were reconstructed at each frequency on the basis of computational models of light diffusion, current flow, and microwave propagation. Electromagnetic properties were correlated with subject characteristics and between techniques. Each technique yielded information on breast tissue features (eg, conductivity, permittivity, light scattering, and absorption) that had not previously all been measured in the same individuals.
Major role for sensory feedback in soleus EMG activity in the stance phase of walking in man (2000)

1. Sensory feedback plays a major role in the regulation of the spinal neural locomotor circuitry in cats. The present study investigated whether sensory feedback also plays an important role during walking in 20 healthy human subjects, by arresting or unloading the ankle extensors 6 deg for 210 ms in the stance phase of gait. 2. During the stance phase of walking, unloading of the ankle extensors significantly (P < 0.05) reduced the soleus activity by 50 % in early and mid-stance at an average onset latency of 64 ms. 3. The onset and amplitude of the decrease in soleus activity produced by the unloading were unchanged when the common peroneal nerve, which innervates the ankle dorsiflexors, was reversibly blocked by local injection of lidocaine (n = 3). This demonstrated that the effect could not be caused by a peripherally mediated reciprocal inhibition from afferents in the antagonist nerves. 4. The onset and amplitude of the decrease in soleus activity produced by the unloading were also unchanged when ischaemia was induced in the leg by inflating a cuff placed around the thigh. At the same time, the group Ia-mediated short latency stretch reflex was completely abolished. This demonstrated that group Ia afferents were probably not responsible for the decrease of soleus activity produced by the unloading. 5. The findings demonstrate that afferent feedback from ankle extensors is of significant importance for the activation of these muscles in the stance phase of human walking. Group II and/or group Ib afferents are suggested to constitute an important part of this sensory feedback.
Methicillin-resistant Staphylococcus aureus strain USA300: origin and epidemiology (2009)

Methicillin-resistant Staphylococcus aureus (MRSA) PFGE strain type USA300 (multilocus sequence type 8, clonal complex 8, staphylococcal cassette chromosome mec type IV) was first reported in the USA as a cause of skin and soft issue infection among college football players in Pennsylvania and among prisoners in Missouri in 2000. Over the next 5 years, USA300 became the predominant community-associated MRSA strain in the USA. It was the most common PFGE type recovered from skin and soft tissue infections in persons presenting to 11 emergency departments across the USA, and caused outbreaks in Native American populations, children in daycare centres, military recruits, prison inmates and among men who have sex with men. Although predominantly a cause of skin and soft issue infection, USA300 isolates also have been recovered from cases of invasive disease including bacteraemia, endocarditis, severe necrotizing pneumonia and osteomyelitis. Isolates of USA300 usually carry the genes encoding the Panton-Valentine leucocidin and the arginine catabolic mobile element, but rarely carry staphylococcal enterotoxin genes. USA300 isolates are becoming more resistant to antimicrobial agents, including erythromycin, levofloxacin, mupirocin and tetracycline, and have spread to Europe, South America and Australia. The emergence of the MRSA USA300 strain type represents a unique biological success story.
Discrimination among the human beta A, beta S, and beta C-globin genes using allele-specific oligonucleotide hybridization probes. (1985)

Synthetic nonadecanucleotides complementary to the human beta A-, beta S-, or beta C-globin sequences were used as hybridization probes to screen human genomic DNA samples for these genes. The oligonucleotides were 32P-labeled and used as probes to genotype restriction endonuclease digests of human genomic DNA. The data obtained show that hybridization with oligonucleotide probes, unlike restriction fragment length polymorphism (RFLP) analysis or direct restriction enzyme digestion, can be used to directly distinguish among the three alleles of beta-globin, beta A, beta S, and beta C, when present either in one (heterozygous) or two copies.
Invader® Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation (2006)

The Invader assay (Third Wave Technologies) is a homogeneous, isothermal DNA probe-based method for sensitive detection of nucleic acid sequences. Invader reactions are performed directly on genomic DNA or total RNA targets; however, polymerase chain reaction- or reverse transcriptase polymerase chain re action-amplified products can also be used. Detection is achieved through target-specific signal amplification instead of target amplification. The assay is a highly accurate and specific detection method for both qualitative and quantitative analysis of single-nucleotide changes, insertions or deletions, gene copy number, infectious agents, and gene expression.
Peroxide generation and decomposition on polymer surface (1995)

Abstract Polymer peroxides were generated onto polypropylene and polyurethane films, and polyester fibers by ozonization, UV irradiation, and plasma treatment. Their thermal and redox decompositions were studied using two assays: peroxidase and iodide. They may differ in the sensitivity to access the peroxides, depending on their localization in the polymer substrates. It was found that the generated peroxides are likely to be distributed not only on the outermost surface, but also in the subsurface of the polymer substrates. Plasma treatment provided polyurethane film with easily accessible peroxides, while UV irradiation and ozonization generated peroxides that were mostly incapable of reacting with aqueous solution of peroxidase. Redox decomposition of the peroxide groups by ferrous ions at 25°C showed that less than 50% of peroxides could react with ferrous ions with a rate constant similar to that of hydrogen peroxide in aqueous solution. Thermal decomposition of the peroxides did not obey the first‐order kinetics, probably due to formation of not single peroxide species with different decomposition rate constants. The lowest decomposition rate constant observed at 65°C was 3 × 10 −3 min −1 , which did not depend on the polymer substrate nature and the method of peroxide generation. © 1995 John Wiley &amp; Sons, Inc.
Retrovirus-delivered siRNA (2002)

The experiments presented here demonstrate that retroviruses are efficient vectors for delivery of siRNA into mammalian cells. Retrovirus-delivered siRNA provides significant advancement over previously available methods by providing efficient, uniform delivery and immediate selection of stable "knock-down" cells. This development should provide a method to rapidly assess gene function in established cell lines, primary cells, or animals.
Data Center Switch Architecture in the Age of Merchant Silicon (2009)

Today, data center networks that scale to tens of thousands of ports require the use of highly-specialized ASICs, with correspondingly high development costs. Simultaneously, these networks also face significant performance and management limitations. Just as commodity processors and disks now form the basis of computing and storage in the data center, we argue that there is an opportunity to leverage emerging high-speed commodity merchant switch silicon as the basis for a scalable, cost-effective, modular, and more manageable data center network fabric. This paper describes how to save cost and power by repackaging an entire data center network as a distributed multi-stage switch using a fat-tree topology and merchant silicon instead of proprietary ASICs. Compared to a fat tree of discrete packet switches, a 3,456-port 10 Gigabit Ethernet realization of our architecture costs 52% less, consumes 31% less power, occupies 84% less space, and reduces the number of long, cumbersome cables from 6,912 down to 96, relative to existing approaches.
Omni-Rig sensors: what can be done with a non-rigid vision platform? (2002)

We describe the principles of building a moving vision platform (a Rig) that once calibrated can thereon self-adjust to changes in its internal configuration and maintain an Euclidean representation of the 3D world using only projective measurements. Formally, we address the question of how to obtain an invariant 3D projective representation from a dynamic collection of cameras. We show that the maximal generality is reached when the rig consists of 5 cameras whose center of projection remain fixed relative to each other during the motion. In other words, the non-rigid component motion may consist of change of internal parameters and relative camera orientations. The configuration reduces to 3 views when the Rig is built using a single physical camera with half-mirrors (beam-splitters) for creating 3 distinct views. We also briefly discuss 2-view configurations using half-mirrors and the principle behind adapting the configuration to allow for zoom lenses in the system. The new research paradigm on non-rigid rigs (we term "Omni-Rig") is applicable to the design of Vision-based sensors that after calibration can move in space while changing critical elements of their configuration-such as changing focus on the fly, zoom, relative camera orientation and inclination of focal plane to object's surface orientation-without the need for recalibration, i.e., using only projective calculations throughout its motion.
Manifold sequencing: efficient processing of large sets of sequencing reactions. (1994)

Automated instruments for DNA sequencing greatly simplify data collection in the Sanger sequencing procedure. By contrast, the so-called front-end problems of preparing sequencing templates, performing sequencing reactions, and loading these on the instruments remain major obstacles to extensive sequencing projects. We describe here the use of a manifold support to prepare and perform sequencing reactions on large sets of templates in parallel, as well as to load the reaction products on a sequencing instrument. In this manner, all reaction steps are performed without pipetting the samples. The strategy is applied to sequencing PCR-amplified clones of the human mitochondrial D-loop and for detection of heterozygous positions in the human major histocompatibility complex class II gene HLA-DQB, amplified from genomic DNA samples. This technique will promote sequencing in a clinical context and could form the basis of more efficient genomic sequencing strategies.
Predicting the Skilled Use of Hierarchical Menus With the Keystroke-Level Model (1993)

This article addresses a key question in the application of Card, Moran, and Newell's (1983) keystroke-level model to software in which users specify a command by working through a system of hierarchical menus. For example, to insert a row in Lotus 1-2-3®, the user makes three menu choices: W for worksheet, I for insert, and R for row. In the keystroke-level model, it is assumed that a time-consuming mental operation precedes each command. The question in the application of the keystroke-level model to hierarchical menu systems is whether the keystrokes WIR in the previous example constitute the execution of three commands and thus require three mental operations or whether WIR acts as a single command and requires only one mental operation. Data were collected from four highly experienced Lotus 1-2-3 users as they went about their day-to-day work. Strong evidence that only one mental operation is involved in choosing from a hierarchical menu system was obtained. We hypothesize that the discrepancy of our results from the data of others is due to the fact that our subjects were more experienced. The implications of our findings for the design of menus is discussed.