gebhart/patrepeval huggingface dataset:
Induction of cytotoxicity in resting human T lymphocytes bound to tumor cells by antibody heteroconjugates. (1987)

An in vitro model for peripheral human T-cell activation and resultant tumor cell killing is described. Cytotoxic T lymphocytes may be generated from resting lymphocytes by incubation of human peripheral blood mononuclear cells for 3 days with the anti-CD3 monoclonal antibody OKT3. Cytotoxicity in peripheral blood mononuclear cells can also be induced by adding an anti-target-OKT3 antibody conjugate and 10% (vol/vol) fetal calf serum to the culture medium. Conjugate activation of T cells was almost completely blocked, however, when 20% (vol/vol) human serum was added to the medium. Conjugate-mediated peripheral blood mononuclear cells activation was restored to some extent by the addition of melanoma target cells to the culture and was markedly enhanced by a second conjugate containing anti-target cell and anti-CD28 antibody. Monoclonal antibody 9.3 (anti-CD28) provides a progression signal in T-lymphocyte activation when used in combination with anti-CD3. Thus, presentation by the tumor target cells of anti-CD3 and anti-CD28 to resting human lymphocytes causes T-cell activation, which is independent of monocytes, proceeds in the presence of human serum, and results in tumor cell killing.
High affinity interleukin-6 receptor is a hexameric complex consisting of two molecules each of interleukin-6, interleukin-6 receptor, and gp-130. (1994)

The high affinity human interleukin-6 (IL-6) receptor complex consists of IL-6 and two membrane-associated receptor components, the IL-6 receptor (alpha-subunit) and the high affinity converter and signal transducing molecule, gp-130 (beta-subunit). Recombinant IL-6 and the extracellular ("soluble") components of the IL-6 receptor (sIL-6R) and gp-130 (sgp-130) have been prepared in order to investigate the stoichiometry and binding of these components in the low affinity (IL-6.sIL-6R) and high affinity (IL-6.sIL-6R.sgp-130) IL-6 receptor complexes. Using a combination of size-exclusion chromatography and analytical ultracentrifugation analysis, in the low affinity receptor complex, IL-6 was shown to bind sIL-6R in a stoichiometric ratio of 1:1, whereas the high affinity ternary complex is hexameric consisting of two molecules each of IL-6, sIL-6R, and sgp-130. This is the first direct demonstration of a higher order arrangement for receptor cytokine interactions that exhibit both high and low affinity complexes.
Mechanism of Ion Permeation in a Model Channel:  Free Energy Surface and Dynamics of K+ Ion Transport in an Anion-Doped Carbon Nanotube (2006)

The mechanism of the ion permeation is investigated for an anion-doped carbon nanotube, as a model of the K+ channel, by analyzing the free energy surface and the dynamics of the ion permeation through the model channel. It is found that the main rate-determining step is how an ion enters the channel. The entrance of the ion is mostly blocked by a water molecule located at this entrance. Only about 10% of K+ ions which reach the mouth of the channel can really enter the channel. The rejection rate sensitively depends on the location of this water molecule, which is easily controlled by the charge of the carbon nanotube; for example, the maximum permeation is obtained when the anion charge is at a certain value, -5.4e in the present model. At this charge, the facile translocation of the ion inside the channel is also induced due to the number of fluctuations of the ions inside the channel. Therefore, the so-called "Newton's balls", a toy model, combined with a simple ion diffusion model for explaining the fast ion permeation should be modified. The present analysis thus suggests that there exists an optimum combination of the length and the charge of the carbon nanotube for the most efficient ion permeation.
Hormone receptor positive, HER2 negative metastatic breast cancer: A systematic review of the current treatment landscape (2016)

Endocrine therapy for the treatment of hormone receptor positive, HER2 negative, metastatic breast cancer is continually evolving. We systematically reviewed phase 2 and 3 randomized controlled trials (RCTs) of agents used in this setting to assess the effectiveness and safety of these agents for postmenopausal women. Across the 32 studies in more than 10,000 patients, the greatest improvement in progression-free survival (PFS) was seen with the addition of a cyclin-dependent kinase (CDK)4/6 inhibitor to standard endocrine therapy. Treatment with a mammalian target of rapamycin (mTOR) inhibitor, phosphoinositol-3-kinase (Pi3K) inhibitor, vascular endothelial growth factor (VEGF) inhibitor and with a selective estrogen receptor degrader (SERD) also showed benefit in PFS for selected trials. Overall survival (OS) improved with the use of mTOR inhibitors and a SERD; however, studies were not powered for an OS endpoint. Encouraging results from early studies of histone deacetylase (HDAC) and B-cell lymphoma (BCL2) inhibitors are yet to be confirmed in phase III clinical trials. Study discontinuation rates and toxicity-related deaths were highest with VEGF inhibitors in combination with endocrine therapy, limiting their use in hormone receptor positive breast cancer. CDK4/6 inhibitors and mTOR inhibitors appeared to have activity in both first and second line settings, but required additional monitoring for common toxicities. The activity of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors was limited to the first-line setting and treatment discontinuation rates were higher than with mTOR inhibitors and SERDs. Overall, PFS benefit appears to be greatest when agents acting on CDK4/6, mTOR and Pi3K pathways, and SERDs are added to standard endocrine therapy. If these early results persist in further studies, these data are likely to change the way we treat hormone receptor positive, HER2 negative metastatic breast cancer. In the follow-up article to this review, we will consider the potential future treatment options for these patients.
Advanced combustion technologies for gas turbine power plants (1995)

During the second half of fiscal year 1995 progress was made in all three funded subject areas of the project as well as in a new area. Work in the area of mixing and combustion management through flow actuation was transferred into an enclosed facility. Jet mixing in a ducted co-flow was examined. The same jets were also subjected to a strong acoustic field established in the duct. Excitation of the jet with static spatial modes was shown to be effective even in the presence of co-flow and the acoustic field. Only when a wall is placed at the jet exit plane did the acoustic field dominate the jet dispersion (as expected due to reflective boundary conditions and the jet shear layer receptivity). This case is, however, not the most relevant to gas turbine combustors since it precludes co-flow. In the area of combustor testing, the design, fabrication, and assembly of a modular combustor test rig for project has been completed at the University of Arkansas. In the area of high temperature piezoceramic actuator materials development, Sr{sub 2}(Nb{sub x}Ta{sub 1-x}){sub 2}O{sub 7} powders have been synthesized, and bulk samples and thick films sintered. These materials have a curie temperature of about 1400{degrees}C compared with 300{degrees}C for the commercially available PZT. While at room temperature the new materials show a piezoelectric constant (d{sub 33}) which is a factor of 100 lower than PZT, at high temperatures they can exhibit significant action. A new area of non-linear, neural-net based, controllers for mixing and combustion control has been added during the second contract year. This work is not funded by the contract. Significant progress was made in this area. Neural nets with up to 15 neurons in the hidden layer were trained with experimental data and also with data generated using linear stability theory. System ID was performed successfully. The network was then used to predict the behavior of jets excited at other modes not used for the training.
The effects of formulation and moisture on the stability of a freeze-dried monoclonal antibody-vinca conjugate: a test of the WLF glass transition theory. (1992)

Deacetylvinblastine (DAVLB) hydrazide, a cytotoxic vinca alkaloid, has been linked to the monoclonal antibody, KS1/4, via aldehyde residues of the oxidized carbohydrate groups on the antibody. The resulting KS1/4-DAVLB hydrazide conjugate is unstable in solution with both the acyl hydrazone linkage and the vinca moiety being subject to significant degradation, even at 5 degrees C. This necessitated the development of a freeze-dried formulation of the antibody-drug conjugate. Formulation factors considered were pH, ionic strength, buffer, excipient types, and excipient ratios. A formulation with equal weight ratios of mannitol, glycine, and conjugate in a low ionic strength phosphate buffer at near neutral pH was selected. Stability was studied at various moisture levels (1.4%, 3.0%, and 4.7%) and temperatures (5 degrees C, 25 degrees C, and 40 degrees C). Degradation was measured by size exclusion HPLC (aggregate formation) and by reverse phase HPLC (hydrolysis of hydrazone linkage and vinca decomposition). Differential scanning calorimetry (DSC) indicated that all samples were above their glass transition temperatures, Tg, when stored at 40 degrees C. When stored at 25 degrees C, only the highest moisture sample was initially above its Tg. However, due to crystallization of the excipients during storage and the resulting decrease in Tg, samples stored at 25 degrees C were also above their Tg during much of the storage period. The degradation rate, R, increased sharply with increasing temperature and with increasing moisture level. Degradation kinetics obeyed the Williams-Landel-Ferry relationship, R/Rg = exp[k(T-Tg)], where Rg is the degradation rate at Tg. For all three moisture levels and all three degradation pathways, k = 0.143.
Algorithm 548: Solution of the Assignment Problem [H] (1980)

article Free AccessArtifacts AvailableArtifacts Evaluated & ReusableAlgorithm 548: Solution of the Assignment Problem [H] Authors: Giorgio Carpaneto Istituto di Automatica, Facoltà di Ingegneria, Universita Di Bologna, Viale Risorgimento 2, 40136 Bologna, Italy Istituto di Automatica, Facoltà di Ingegneria, Universita Di Bologna, Viale Risorgimento 2, 40136 Bologna, ItalyView Profile , Paolo Toth Istituto di Automatica, Facoltà di Ingegneria, Universita Di Bologna, Viale Risorgimento 2, 40136 Bologna, Italy Istituto di Automatica, Facoltà di Ingegneria, Universita Di Bologna, Viale Risorgimento 2, 40136 Bologna, ItalyView Profile Authors Info & Claims ACM Transactions on Mathematical SoftwareVolume 6Issue 1pp 104–111https://doi.org/10.1145/355873.355883Published:01 March 1980Publication History 80citation1,525DownloadsMetricsTotal Citations80Total Downloads1,525Last 12 Months60Last 6 weeks13 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Three Novel Hydroxybenzoate Saxitoxin Analogues Isolated from the Dinoflagellate Gymnodinium catenatum (2003)

In a recent survey of paralytic shellfish poisoning (PSP) toxins in Gymnodinium catenatum Graham extracts, using LC with postcolumn oxidation and fluorescence detection, three novel saxitoxin analogues were revealed in isolates from several locations, including Australian waters. We have named them as G. catenatum toxins, GC1 (1), GC2 (2), and GC3 (3). The compounds were isolated from a culture of the Australian strain by LC-MS-guided fractionation employing a C18-silica column and hydrophilic interaction chromatography. The unusual structures of these novel compounds were characterized by low- and high-resolution MS, MS/MS, and NMR spectroscopy. GC3 (3) was found to be the 4-hydroxybenzoate ester derivative of decarbamoylsaxitoxin, while GC1 (1) and GC2 (2) are the epimeric 11-hydroxysulfate derivatives of GC3 (3).
Gold-coated iron nanoparticles: a novel magnetic resonance agent forT1andT2weighted imaging (2006)

Core/shell structured iron(Fe)/gold(Au) nanoparticles have been prepared by a reverse micelle method, and their potential application as magnetic resonance (MR) contrast agent investigated. The average nanoparticle size is 19 nm, as determined by x-ray powder diffraction (XRD) and transmission electron microscopy (TEM). Magnetic properties and relaxivities of nanoparticles are presented and compared. The saturation magnetization is 81 emu g−1 Fe for freshly prepared nanoparticles and the particles have high r1(6.87 mM−1 s−1) when the Fe core is kept from oxidation. These nanoparticles may be used as T1 agents in the unoxidized form.
A large-eddy simulation of turbulent compressible convection: differential rotation in the solar convection zone (2001)

We present the results of two simulations of the convection zone, obtained by solving the full hydrodynamic equations in a section of a spherical shell. The first simulation has cylindrical rotation contours (parallel to the rotation axis) and a strong meridional circulation, which traverses the entire depth. The second simulation has isorotation contours about mid-way between cylinders and cones, and a weak meridional circulation, concentrated in the uppermost part of the shell. We show that the solar differential rotation is directly related to a latitudinal entropy gradient, which pervades into the deep layers of the convection zone. We also offer an explanation of the angular velocity shear found at low latitudes near the top. A non-zero correlation between radial and zonal velocity fluctuations produces a significant Reynolds stress in that region. This constitutes a net transport of angular momentum inwards, which causes a slight modification of the overall structure of the differential rotation near the top. In essence, the thermodynamics controls the dynamics through the Taylor-Proudman momentum balance. The Reynolds stresses only become significant in the surface layers, where they generate a weak meridional circulation and an angular velocity ‘bump’.
Estimations of the increasing prevalence and plateau prevalence of chronic myeloid leukemia in the era of tyrosine kinase inhibitor therapy (2012)

The current results indicated that the prevalence of CML will continue to increase to reach a near plateau prevalence 35 times the annual incidence. These estimates should be considered in health care policies and in the design of future studies in CML.
Study on Material Management System of Coal Enterprise Based on Data Warehouse (2004)

On the bas is of the reality of material supply management of the coal enterprise, this paper expounds plans of material management systems based on specific IT, and indicates the deficiencies, the problems of them and the necessity of improving them. The structure, models and data organizing schema of the material management decision support system are investigated based on a new data management technology (data warehousing technology).
Technology in patient care (1999)

It is incumbent on healthcare professionals to ensure that the criteria of “better”, “cheaper” and “more effective” are satisfied
Non-invasive prenatal diagnosis (2006)

Obtaining fetal material for molecular analysis without the need for invasive procedures has been a goal of fetal medicine for many years. This is becoming a reality now that circulating cell-free fetal nucleic acids can be detected in maternal plasma. Tests are already being used in clinical practice for the management of X-linked conditions through the determination of fetal sex and for red cell alloimmunisation by prediction of fetal rhesus D status. The sensitivity of this technique produces highly accurate results, thereby reducing the number of invasive procedures needed. This approach has also been used in the diagnosis of genetic disorders and in obstetric complications where the level of free fetal DNA may be a marker for placental abnormalities.
Requirement for Activation of the Serine-Threonine Kinase Akt (Protein Kinase B) in Insulin Stimulation of Protein Synthesis but Not of Glucose Transport (1998)

A wide variety of biological activities including the major metabolic actions of insulin is regulated by phosphatidylinositol (PI) 3-kinase. However, the downstream effectors of the various signaling pathways that emanate from PI 3-kinase remain unclear. Akt (protein kinase B), a serine-threonine kinase with a pleckstrin homology domain, is thought to be one such downstream effector. A mutant Akt (Akt-AA) in which the phosphorylation sites (Thr308 and Ser473) targeted by growth factors are replaced by alanine has now been shown to lack protein kinase activity and, when overexpressed in CHO cells or 3T3-L1 adipocytes with the use of an adenovirus vector, to inhibit insulin-induced activation of endogenous Akt. Akt-AA thus acts in a dominant negative manner in intact cells. Insulin-stimulated protein synthesis, which is sensitive to wortmannin, a pharmacological inhibitor of PI 3-kinase, was abolished by overexpression of Akt-AA without an effect on amino acid transport into the cells, suggesting that Akt is required for insulin-stimulated protein synthesis. Insulin activation of p70 S6 kinase was inhibited by approximately 75% in CHO cells and approximately 30% in 3T3-L1 adipocytes, whereas insulin-induced activation of endogenous Akt was inhibited by 80 to 95%, by expression of Akt-AA. Thus, Akt activity appears to be required, at least in part, for insulin stimulation of p70 S6 kinase. However, insulin-stimulated glucose uptake in both CHO cells and 3T3-L1 adipocytes was not affected by overexpression of Akt-AA, suggesting that Akt is not required for this effect of insulin. These data indicate that Akt acts as a downstream effector in some, but not all, of the signaling pathways downstream of PI 3-kinase.
Differential Regulation of the Cellulase Transcription Factors XYR1, ACE2, and ACE1 in Trichoderma reesei Strains Producing High and Low Levels of Cellulase (2010)

Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly being investigated for second-generation biofuel production from lignocellulosic biomass. The induction mechanisms of T. reesei cellulases have been described recently, but the regulation of the genes involved in their transcription has not been studied thoroughly. Here we report the regulation of expression of the two activator genes xyr1 and ace2, and the corepressor gene ace1, during the induction of cellulase biosynthesis by the inducer lactose in T. reesei QM 9414, a strain producing low levels of cellulase (low producer). We show that all three genes are induced by lactose. xyr1 was also induced by d-galactose, but this induction was independent of d-galactose metabolism. Moreover, ace1 was carbon catabolite repressed, whereas full induction of xyr1 and ace2 in fact required CRE1. Significant differences in these regulatory patterns were observed in the high-producer strain RUT C30 and the hyperproducer strain T. reesei CL847. These observations suggest that a strongly elevated basal transcription level of xyr1 and reduced upregulation of ace1 by lactose may have been important for generating the hyperproducer strain and that thus, these genes are major control elements of cellulase production.
Phase-only modulation with twisted nematic liquid-crystal spatial light modulators (1988)

It is shown that twisted nematic liquid-crystal spatial light modulators behave as phase-only modulators when operated below the conventional optical threshold. Thus such devices, when operated in a reflection mode, behave as spatial amplitude modulators when used between crossed polarizers above the optical threshold; they behave as phase modulators when used between parallel polarizers and operated below that threshold.
Algorithms for image processing and computer vision (1996)

A cookbook of algorithms for common image processing applications Thanks to advances in computer hardware and software, algorithms have been developed that support sophisticated image processing without requiring an extensive background in mathematics. This bestselling book has been fully updated with the newest of these, including 2D vision methods in content-based searches and the use of graphics cards as image processing computational aids. It's an ideal reference for software engineers and developers, advanced programmers, graphics programmers, scientists, and other specialists wh
CD34 expression on platelets (2003)

Flow cytometric analysis of human platelets from healthy volunteers by high-resolution immunophenotyping accomplished with enzymatic amplification staining revealed the expression of CD34. Assessment of the platelets with five different monoclonal antibodies to the three different classes of CD34 epitopes suggested that platelets express a unique glycoform of the molecule. Expression of CD34 was verified by immunoblotting. Platelet activation resulted in a marked decrease in surface CD34. CD34 expression has significant implications for the function of platelets as well as for the determination of hematopoietic progenitor cells.
The evaporation of drops from super-heated nano-engineered surfaces (2009)

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2009.
Structure and Stoichiometry in Supervalent Doped Li7La3Zr2O12 (2015)

The oxide garnet material Li7La3Zr2O12 shows remarkably high ionic conductivity when doped with supervalent ions that are charge compensated by Li vacancies and is currently one of the best candidates for development of a technologically relevant solid electrolyte. Determination of optimal dopant concentration, however, has remained a persistent problem due to the extreme difficulty of establishing the actual (as compared to nominal) stoichiometry of intentionally doped materials and by the fact that it is still not entirely clear what level of lattice expansion/contraction best promotes ionic diffusion. By combining careful synthesis, neutron diffraction, high-resolution X-ray diffraction (XRD), Raman measurements, and density functional theory calculations, we show that structure and stoichiometry are intimately related such that the former can in many cases be used as a gauge of the latter. We show that different Li-vacancy creating supervalent ions (Al3+ vs Ta5+) affect the structure very differently, both in terms of the lattice constant, which is easily measurable, and in terms of the local structure, which can be difficult or impossible to access experimentally but may have important ramifications for conduction. We carefully correlate the lattice constant to dopant type/concentration via Vegard’s law and then further correlate these quantities to relevant local structural parameters. Our work opens the possibility of developing a codopant scheme that optimizes the Li vacancy concentration and the lattice size simultaneously.