gebhart/patrepeval huggingface dataset:
Highly Protein-Resistant Coatings from Well-Defined Diblock Copolymers Containing Sulfobetaines (2006)

Three well-defined diblock copolymers of poly(sulfobetaine methacrylate) [poly(SBMA)] and poly(propylene oxide) (PPO) were synthesized by the sequential addition of SBMA monomer to fixed amounts of PPO using an atom transfer radical polymerization method and varying poly(SBMA) lengths. These copolymers were characterized by 1H NMR and aqueous gel permeation chromatography. These copolymers were physically adsorbed onto a surface plasmon resonance (SPR) sensor surface covered by methyl-terminated self-assembled monolayers, followed by the in situ evaluation of protein adsorption on the adsorbed copolymers. It is found that the behavior of the protein adsorption depends on the molecular weight of the copolymers. Results show that the diblock copolymers containing poly(SBMA) can be highly protein resistant when surface SBMA densities are well controlled. Thus, copolymers containing zwitterionic groups are ideal for resisting protein adsorption when the surface density of zwitterionic groups is controlled.
DNA Methylation in Circulating Tumour DNA as a Biomarker for Cancer (2007)

Free circulating DNA, which is thought to be derived from the primary tumour, can be detected in the blood of patients with cancer. Detection of genetic and epigenetic alteration in this tumour DNA offers a potential source of development of prognostic and predictive biomarkers for cancer. One such change is DNA methylation of the promotor region of tumour suppressor genes. This causes down regulation of tumour suppressor gene expression, a frequent event in carcinogenesis. Hypermethylation of the promotor region of a number of genes has been detected in many tumour types and more recently these changes have been detected in circulating tumour DNA. This review will summarise the literature detailing DNA methylation in circulating tumour DNA and discuss some of the current controversies and technical challenges facing its use as a potential biomarker for cancer.
Cross‐reactivity of acid‐sensing ion channel and Na + –H + exchanger antagonists with nicotinic acetylcholine receptors (2011)

Nicotinic acetylcholine receptors (nAChRs) are widely distributed throughout the mammalian central and peripheral nervous systems, where they contribute to neuronal excitability and synaptic communication. It has been reported that nAChRs are modulated by BK channels and that BK channels, in turn, are inhibited by acid-sensing ion channels (ASICs). Here we investigate the possible functional interaction between these channels in medial habenula (MHb) neurones. We report that selective antagonists of large-conductance calcium-activated potassium channels and ASIC1a channels, paxilline and psalmotoxin 1, respectively, did not induce detectable changes in nicotine-evoked currents. In contrast, the non-selective ASIC and Na(+)-H(+) exchanger (NHE1) antagonists, amiloride and its analogues, suppressed nicotine-evoked responses in MHb neurones of wild-type and ASIC2 null mice, excluding a possible involvement of ASIC2 in the nAChR inhibition by amiloride. Zoniporide, a more selective inhibitor of NHE1, reversibly inhibited α3β4-, α7- and α4-containing (*) nAChRs in Xenopus oocytes and in brain slices, as well as in PS120 cells deficient in NHE1 and virally transduced with nAChRs, suggesting a generalized effect of zoniporide in most neuronal nAChR subtypes. Independently from nAChR antagonism, zoniporide profoundly blocked synaptic transmission onto MHb neurones without affecting glutamatergic and GABA receptors. Taken together, these results indicate that amiloride and zoniporide, which are clinically used to treat hypertension and cardiovascular disease, have an inhibitory effect on neuronal nAChRs when used experimentally at high doses. The possible cross-reactivity of these compounds with nAChRs in vivo will require further investigation.
CSE1L/CAS, the cellular apoptosis susceptibility protein, enhances invasion and metastasis but not proliferation of cancer cells (2008)

Our results indicate that CAS increases the invasion but not the proliferation of cancer cells. Thus, CAS plus ECM-degradation proteinases may be used as the markers for predicting the advance of tumour metastasis.
Deregulation of DNA-dependent protein kinase catalytic subunit contributes to human hepatocarcinogenesis development and has a putative prognostic value (2013)

DNA-dependent protein kinase catalytic subunit transduces HSF1 effects in HCC cells, and might represent a novel target and prognostic factor in human HCC.
Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. (1981)

A clonal hamster beta cell line (HIT) was established by simian virus 40 transformation of Syrian hamster pancreatic islet cells. Cytoplasmic insulin was detected in all cells by indirect fluorescent antibody staining, and membrane-bound secretory granules were observed ultrastructurally. Acidified-ethanol extracts of HIT cell cultures contained hamster insulin as determined by radioimmunoassay, radioreceptor assay, and bioassay. One subclone at passage 39 contained 2.6 micrograms of insulin per mg of cell protein. [3H]Leucine-labeled HIT insulin and proinsulin were identical to islet-derived proteins when compared by NaDodSO4/polyacrylamide gel electrophoresis of immunoprecipitates. HIT cell insulin secretion was stimulated by glucose, glucagon, and 3-isobutyl-1-methylxanthine. Insulin secretion at optimal glucose concentration (7.5 mM) was 2.4 milliunits per 10(6) cells per hr. Somatostatin and dexamethasone markedly inhibited HIT insulin secretion. The HIT cell line represents a unique in vitro system for studying beta cell metabolism and insulin biosynthesis.
HEALTH CARE IN FOCUS (2004)

RETURN TO ISSUEPREVNewsNEXTHEALTH CARE IN FOCUSThe pharmaceutical industry is seeking a new prescription for success as it faces pricing pressures, challenges from generics, and consumer disenchantmentCite this: Chem. Eng. News 2004, 82, 49, 18–29Publication Date (Print):December 6, 2004Publication History Published online14 November 2010Published inissue 6 December 2004https://doi.org/10.1021/cen-v082n049.p018Copyright © 2004 American Chemical SocietyArticle Views8Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Pharmaceuticals Get e-Alerts
Meclizine and Placebo in Treating Vertigo of Vestibular Origin (1972)

In a double-blind crossover study, meclizine hydrochloride was shown to be significantly more effective than placebo in treating patients with positional and continuous vertigo of vestibular origin. Meclizine reduced the severity and frequency of attacks, as well as signs and symptoms associated with the vertigo. These included nausea, positional and positioning nystagmus, and postural instability. There was no difference in the response of patients with disease of the peripheral or central vestibular system.
Thyroid Hormone Promotes Serine Phosphorylation of p53 by Mitogen-Activated Protein Kinase (2001)

L-Thyroxine (T(4)) nongenomically promotes association of mitogen-activated protein kinase (MAPK) and thyroid hormone receptor TRbeta1 (TR) in the cell nucleus, leading to serine phosphorylation of the receptor. The oncogene suppressor protein, p53, is serine phosphorylated by several kinases and is known to interact with TRbeta1. We studied whether association of p53 and TR is modulated by T(4) and involves serine phosphorylation of p53 by MAPK. TR-replete 293T human kidney cells were incubated with a physiological concentration of T(4) for 10-90 min. Nuclear fractions were immunoprecipitated and the resulting proteins separated and immunoblotted for co-immunoprecipitated proteins. Activated MAPK immunoprecipitates of nuclei from T(4)-treated cells accumulated p53 in a time-dependent manner; T(4) and T(4)-agarose were more effective than T(3). T(4)-induced nuclear complexing of p53 and MAPK was inhibited by PD 98059 (PD) and U0126, two MAPK kinase (MEK) inhibitors, and was absent in cells treated with MEK antisense oligonucleotide and in dominant negative Ras cells. T(4) also caused nuclear co-immunoprecipitation of TRbeta1 and p53, an effect also inhibited by PD. Nuclear complexing of p53 and MAPK also occurred in HeLa cells, which lack functional TR. Constitutively activated MAPK caused phosphorylation of a recombinant p53-GST fusion protein in vitro; thus, p53 is a substrate for MAPK. An indicator of p53 transcriptional activity, accumulation of the immediate-early gene product, c-Jun, was inhibited by T(4). This T(4) effect was reversed by PD, indicating that the transcriptional activity of p53 was altered by T(4)-directed MAPK-p53 interaction.
Loss of heterozygosity at the familial RCC t(3;8) locus in most clear cell renal carcinomas. (1995)

Previously, we had observed that more than 80% of clear cell renal carcinomas (RCCs) exhibited loss of heterozygosity (LOH) between the microsatellite markers D3S1285 (in 3p14.1) and D3S1295 (in 3p21.1), a region which includes the protein tyrosine phosphatase gamma locus (PTPRG locus, PTP gamma gene) and the 3p14.2 break of the familial RCC-associated translocation, t(3;8)(p14.2;q24), which has been hypothesized to affect expression of an RCC suppressor gene or oncogene. Using seven microsatellite markers and four markers derived from a PTPRG YAC contig, we have further delineated the 3p14.2 region of LOH in RCCs. Eighty-nine % of clear cell RCCs (31 of 35) showed a common region of loss between the D3S1481 and D3S1312 loci which flank the 3p14.2 t(3;8) translocation breakpoint and the PTP gamma gene. The PTP gamma gene occupies approximately 780 kilobase pairs between markers D3S1480 and D3S1312, with its currently defined 5' end greater than 200 kilobase pairs centromeric to the 3p14.2 translocation break. Although most of the RCCs with LOH between D3S1481 and D3S1312 loci have lost at least a portion of one PTP gamma allele, we have tested all known exons of the remaining PTP gamma gene in a number of the kidney tumors and have not observed mutations. Thus, there may be another gene in the vicinity of the 3p14.2 break that is important not only in the familial RCCs in the t(3;8) family but in the majority of clear cell RCCs.
Traumatically induced axonal injury: pathogenesis and pathobiological implications. (1992)

This work reviews the pathobiology of traumatically induced axonal injury. Drawing upon literature gleaned from the experimental and clinical setting, this review attempts to emphasize that, other than the most destructive insults, traumatic brain injury does not typically cause direct mechanical disruption of the axon. Rather, this review documents that with traumatic injury focal, subtle axonal change occurs, and that over time, such change leads to impaired axoplasmic transport, continued axonal swelling, and ultimate disconnection. The initial intra-axonal events that trigger the above described sequence of reactive axonal change are considered with focus on the possibility of either traumatically altered axolemmal permeability, direct cytoskeletal damage/perturbation, or more overt metabolic/functional disturbances. Not only does this review focus on the sequence of traumatically induced axonal change, but also, it considers its attendant consequences in terms of Wallerian degeneration and subsequent deafferentation. The concept that traumatically induced diffuse axonal injury leads to diffuse deafferentation is emphasized together with its pathobiological implications for morbidity and recovery. The potential for either adaptive or maladaptive neuroplasticity subsequent to such diffuse deafferentation is considered in the context of mild, moderate and severe traumatic brain injury.
Tissue-Engineering Bioreactors: A New Combined Cell-Seeding and Perfusion System for Vascular Tissue Engineering (2002)

One approach to the tissue engineering of vascular structures is to develop in vitro conditions in order ultimately to fabricate functional vascular tissues before final implantation. In our experiment, we aimed to develop a new combined cell seeding and perfusion system that provides sterile conditions during cell seeding and biomechanical stimuli in order to fabricate autologous human vascular tissue in vitro. The cell seeding and perfusion system is made of Plexiglas and is completely transparent (Berlin Heart, Berlin, Germany; University Hospital Benjamin Franklin, Berlin, Germany). The whole system consists of a cell seeding chamber that can be incorporated into the perfusion system and an air-driven respirator pump connected to the bioreactor. The cell culture medium continuously circulates through a closed-loop system. We thus developed a cell seeding device for static and dynamic seeding of vascular cells onto a polymeric vascular scaffold and a closed-loop perfused bioreactor for long-term vascular conditioning. The cell seeding chamber can be easily connected to the bioreactor, which combines continuous, pulsatile perfusion and mechanical stimulation to the tissue-engineered conduit. Adjusting the stroke volume, the stroke rate, and the inspiration/expiration time of the ventilator allows various pulsatile flows and different levels of pressure. The whole system is a highly isolated cell culture setting, which provides a high level of sterility and a gas supply and fits into a standard humidified incubator. The device can be sterilized by ethylene oxide and assembled with a standard screwdriver. Our newly developed combination of a cell seeding and conditioning device provides sterile conditions and biodynamic stimuli for controlled tissue development and in vitro conditioning of an autologous tissue-engineered vessel.
Comprehensive analysis of gratings for ultraviolet space instrumentation (1978)

Comprehensive measurements in the vacuum uv range of 1200-3000 A of efficiency, polarization, and scattering of classically ruled and photoresist gratings are reported. The results show that the art of ruling gratings for vacuum uv use has reached a high level of sophistication and that careful analysis of grating properties can lead to useful improvement of the ruling art.
A modification of the RSA public-key encryption procedure (Corresp.) (1980)

The Rivest, Shamir, and Adleman (RSA) public-key encryption algorithm can be broken if the integer <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">R</tex> used as the modulus can be factored. It my however be possible to break this system without factoring <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">R</tex> . A modification of the RSA scheme is described. For this modified version it is shown that, if the encryption procedure can be broken in a certain number of operations, then <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">R</tex> can be factored in only a few more operations. Furthermore, this technique can also be used to produce digital signatures, in much the same manner as the RSA scheme.
Non-destructive spectroscopic detection of cobalt(II) in paintings and glass (1996)

AbstractFrom classical times to the present day, pigments containing cobalt(II) have played an intermittent role. The most important cobalt blue pigments were smalt and Thenard's blue. In the present work, fibre optics reflectance spectroscopy (FORS) is proposed as a totally non-destructive method for the detection of cobalt(II) pigments in the visible and near infrared regions. This technique has been applied to the identification of cobalt(II) in a sixteenth-century wall painting and two fifteenth-century glass windows.
Oxygen Deprivation but Not a Combination of Oxygen, Glucose, and Serum Deprivation Induces DNA Degradation in Mouse Cortical Neurons in Vitro: Attenuation by Transgenic Overexpression of CuZn-Superoxide Dismutase (1996)

The present work was designed to study the possible implication of apoptosis in ischemic neuronal death, a phenomenon that has been suggested to be involved in neurodegeneration following focal as well as global ischemia. In this study, mouse cortical neurons in primary culture were subjected to oxygen deprivation or oxygen, glucose, and serum deprivation to simulate hypoxia and "ischemia-like" conditions; also, cellular viability as well as DNA degradation were investigated. The results showed that DNA degradation occurred in neurons subjected to oxygen deprivation but not to oxygen and substrate deprivation together. This DNA degradation, resulting in a laddering by agarose gel electrophoresis, could be prevented by cycloheximide and actinomycin-D treatments, although these inhibitors were unable to reduce neuronal death. To investigate if DNA degradation could be elicited by an intracellular free radical generation during reoxygenation, transgenic neurons overexpressing copper-zinc superoxide dismutase were subjected to 9 h of oxygen deprivation and analyzed after 24 h of reoxygenation. The results showed a significant attenuation of DNA degradation in these cells and confirmed a possible relationship between reactive oxygen species and neuronal apoptosis. This study opens the way to further investigations regarding the involvement of an apoptotic process in necrotic neuronal death, and provides some new insights into the mechanisms underlying selective sensitivity of neuronal cells to oxygen and glucose deprivation.
Single-tube three-dimensional scanner for scanning tunneling microscopy (1986)

We report a new type of three-dimensional mechanical scanner fabricated from a single piezoelectric tube. It has a typical response of 5 nm/V in each orthogonal direction and mechanical resonances at 8 kHz (bending perpendicular to the tube axis) and 40 kHz (motion parallel to the tube axis). When used in a scanning tunneling microscope it is the higher frequency mode which is most critical since it corresponds to motion perpendicular to the sample surface. We show an image of the atomic surface of graphite taken in air using a tube scanner incorporated into a scanning tunneling microscope. The tube scanner allows the development of smaller, simpler, and faster scanning tunneling microscopes.
Software to Monitor CD-ROM Usage. (1989)

The study was aimed for molecular detection of mycobacterial DNA in different clinical samples using real-time polymerase chain reaction (RT-PCR) system and rapid diagnosis of tuberculosis. A total of 508 clinical specimens (blood 343, menstrual fluid 53, endometrial tissue 43, body fluid 36, pus from lymph nodes 18, sputum 8, urine 5 and semen 2) were included in this study. We extracted DNA using QIAamp DNA Mini Kit (QIAGEN, Germany) and performed real-time assay using Rotor-Gene Q machine from Corbett Research, Australia for specific amplification of IS6110 sequence of mycobacterial genome. The RT-PCR result was also compared with bacterial culture and acid-fast bacillus staining. RT-PCR assay showed positivity in 52 cases and negative in 456 cases. Corresponding positive results in culture and acid-fast bacillus staining methods were 49 cases and 24 cases respectively. The sensitivity and specificity of detecting Mycobacterium tuberculosis by RT-PCR were 93.87% and 98.69% respectively taking positive culture results as reference standards. The overall positive and negative predictive values were 88.46% and 99.34% respectively. RT-PCR is a useful diagnostic tool for rapid and sensitive detection of mycobacteria in different clinical samples. The easy processing, fast reporting and relative lack of contamination issues make it worthy as a possible replacement to time consuming culture techniques. Moreover, it has added advantage of quantification of mycobacterial DNA, hence bacterial load.
Thermophotovoltaic generation with selective radiators based on tungsten surface gratings (2004)

Two-dimensional surface-relief gratings with a period of 1.0–0.2μm composed of rectangular microcavities were fabricated on single crystalline W substrates to develop spectrally selective radiators for thermophotovoltaic generation. The radiators displayed strong emission in the near-infrared region where narrow-band-gap photovoltaic cells could convert photons into electricity. The enhancement of thermal emission was attributed to the microcavity effect. Power generation tests were carried out and the W gratings showed more than two times higher generation efficiency, compared to a SiC radiator. The results showed that the microstructured W radiators behave as good selective radiator, with both high efficiency and high power density.
Precise excision and transposition of piggyBac in pink bollworm embryos (1999)

Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti. Plasmid-based excision and transposition assays have been useful indicators of an element's ability to be mobilized in vivo and thus potentially serve as a transforming vector. We report that the transposable element piggyBac is capable of precise excision and transposition in the pink bollworm (Pectinophora gossypiella), a worldwide pest of cultivated cotton. Combined with a suitable marker gene, the piggyBac element may serve as a vector for germline transformation in this and (potentially) other lepidopteran species.
Electroanalysis of Glucose in Transcutaneously Extracted Samples (2000)

Glucose can be extracted through intact skin by electroosmosis upon application of a low-level electrical current. The amount of glucose extracted has been shown previously to correlate with blood glucose level. An amperometric, GOx-based biosensor was used to measure the amount of glucose in samples extracted from a nondiabetic volunteer every twenty minutes for five hours. The blank-subtracted current output from this sensor accurately tracked blood glucose changes and correlated with the capillary blood glucose values (average r>0.93) and a time lag of twenty minutes. This proof-of-feasibility is a prerequisite to the development of an integrated, wearable glucose monitor for diabetics combining both biosensor and iontophoresis functions.
A stacked, voted, stacked model for named entity recognition (2003)

This paper investigates stacking and voting methods for combining strong classifiers like boosting, SVM, and TBL, on the named-entity recognition task. We demonstrate several effective approaches, culminating in a model that achieves error rate reductions on the development and test sets of 63.6% and 55.0% (English) and 47.0% and 51.7% (German) over the CoNLL-2003 standard baseline respectively, and 19.7% over a strong AdaBoost baseline model from CoNLL-2002.