gebhart/patrepeval huggingface dataset:
Macrophage Activation in Atherosclerosis: Pathogenesis and Pharmacology of Plaque Rupture (2005)

Atherosclerosis is still an important disease. It accounts for 39% of deaths in the U.K. and 12 million U.S citizens have atherosclerosis-associated disease. Atherosclerosis may exert clinical effects by slow narrowing, producing stable angina or dramatic rupture, producing acute coronary syndromes such as unstable angina or myocardial infarction and death. Macrophages are abundant in ruptured atherosclerotic plaques. Macrophages are innate immune effectors, i.e. they are activated without antigenic specificity. This may make them liable to indiscriminate tissue damage, since they are less selective than lymphocytes. Macrophages are recruited and activated by many signals and have an impressive armamentarium of molecules to promote tissue damage. Macrophage recruitment by abnormal endothelium over developing atherosclerotic plaques, is aided by endothelial expression of adhesion molecules (ICAM-1, VCAM, ELAM). Use of knockout mice has implicated the chemoattractant cytokine (chemokine) MCP-1 in attracting macrophage recruitment in atherosclerosis. Macrophage-activation stimuli associated with atherosclerotic risk factors include oxidised low density lipoprotein (oxLDL, "bad cholesterol"), advanced glycosylation end products (AGEs) of diabetes, angiotensin II and endothelin. Substantial work has clarified macrophage activation by OxLDL via macrophage scavenger receptors (MSRs), especially MSRA and CD36. Activated macrophages express effector molecules that kill cells and degrade extracellular matrix. These include Fas-L and nitric oxide (NO). Macrophage NO is derived from the high output inducible nitric oxide synthase (iNOS) pathway and upregulates vascular smooth muscle (VSMC) cell surface Fas, priming them for apoptosis. Activated macrophages express surface Fas-L, similar to cytotoxic T-lymphocytes and natural killer cells. Since VSMCs promote plaque stability, VSMC apoptosis may promote plaque rupture. Macrophages express multiple metalloproteinases (e.g. stromelysin) and serine proteases (e.g. urokinase) that degrade the extracellular matrix, weakening the plaque and making it rupture prone. Macrophages secrete numerous other effectors including reactive oxygen species, eicosanoids, tumour necrosis factor alpha and interleukin-1. Macrophage-derived transforming growth factor beta promotes fibrosis. Existing cardiovascular treatments including angiotensin II receptor antagonists and angiotensin converting enzyme inhibitors, aspirin, cholesterol reduction agents especially statins may inhibit macrophages. The interaction of NO-donors with macrophages and apoptosis is complex and bifunctional. Traditional anti-inflammatory agents such as glucocorticoids and cyclophosphamide have very serious side effects and are probably inappropriate. Novel anti-inflammatory agents e.g. new immunosuppressives and anti-TNF therapy may have an improved cost-benefit ratio.
A Hybrid Hydrogel Biomaterial by Nanogel Engineering: Bottom‐Up Design with Nanogel and Liposome Building Blocks to Develop a Multidrug Delivery System (2012)

New hybrid poly(ethylene glycol) (PEG) hydrogels crosslinked with both nanogels and nanogel-coated liposome complexes are obtained by Michael addition of the acryloyl group of a cholesterol-bearing pullulan (CHP) nanogel to the thiol group of pentaerythritol tetra(mercaptoethyl) polyoxyethylene. The nanogel-coated liposome complex is stably retained after gelation and the complexes are well dispersed in the hybrid gel. Microrheological measurements show that the strength and gelation time of the hybrid hydrogel can be controlled by changing the liposome:nanogel ratio. The hydrogel is gradually degraded by hydrolysis under physiological conditions. In this process, the nanogel is released first, followed by the nanogel-coated liposomes. Hybrid hydrogels that can incorporate various molecules into the nanogel and liposomes, and release them in a two-step controllable manner, represent a new functional scaffold capable of delivering multiple drugs, proteins or DNA.
Microglia and multiple sclerosis (2005)

Microglia participate in all phases of the multiple sclerosis (MS) disease process. As members of the innate immune system, these cells have evolved to respond to stranger/danger signals; such a response within the central nervous system (CNS) environment has the potential to induce an acute inflammatory response. Engagement of Toll-like receptors (TLRs), a major family of pattern-recognition receptors (PRRs), provides an important mechanism whereby microglia can interact with both exogenous and endogenous ligands within the CNS. Such interactions modulate the capacity of microglia to present antigens to cells of the adaptive immune system and thus contribute to the initiation and propagation of the more sophisticated antigen-directed responses. This inflammatory response introduces the potential for bidirectional feedback between CNS resident and infiltrating systemic cells. Such interactions acquire particular relevance in the era of therapeutics for MS because the infiltrating cells can be subjected to systemic immunomodulatory therapies known to change their functional properties. Phagocytosis by microglia/macrophages is a hallmark of the MS lesion; however, the extent of tissue damage and the type of cell death will dictate subsequent innate responses. Microglia/macrophages are armed with a battery of effector molecules, such as reactive nitrogen species, that may contribute to CNS tissue injury, specifically to the injury of oligodendrocytes that is associated with MS. A therapeutic challenge is to modulate the dynamic properties of microglia/macrophages so as to limit potentially damaging innate responses, to protect the CNS from injury, and to promote local recovery.
Resonant-cavity enhanced thermal emission (2005)

In this paper we present a vertical-cavity enhanced resonant thermal emitter—a highly directional, narrow-band, tunable, partially coherent thermal source. This device enhances thermal emittance of a metallic or any other highly reflective structure to unity near a cavity resonant frequency. The structure consists of a planar metallic surface (e.g., silver, tungsten), a dielectric layer on top of the metal that forms a vertical cavity, followed by a multilayer dielectric stack acting as a partially transparent cavity mirror. The resonant frequency can easily be tuned by changing the cavity thickness (thus shifting resonant emission peak), while the angle at which the maximum emittance appears can be tuned as well by changing the number of dielectric stack layers. The thermal emission exhibits an extremely narrow angular emission lobe, suggesting increased spatial coherence. Furthermore, we show that we can enhance the thermal emission of an arbitrarily low-emittance material, choosing a properly designed thermal cavity, to near unity.
Gum Arabic as a Phytochemical Construct for the Stabilization of Gold Nanoparticles: In Vivo Pharmacokinetics and X‐ray‐Contrast‐Imaging Studies (2007)

Gold nanoparticles (AuNPs) have exceptional stability against oxidation and therefore will play a significant role in the advancement of clinically useful diagnostic and therapeutic nanomedicines. Despite the huge potential for a new generation of AuNP-based nanomedicinal products, nontoxic AuNP constructs and formulations that can be readily administered site-specifically through the intravenous mode, for diagnostic imaging by computed tomography (CT) or for therapy via various modalities, are still rare. Herein, we report results encompassing: 1) the synthesis and stabilization of AuNPs within the nontoxic phytochemical gum-arabic matrix (GA-AuNPs); 2) detailed in vitro analysis and in vivo pharmacokinetics studies of GA-AuNPs in pigs to gain insight into the organ-specific localization of this new generation of AuNP vector, and 3) X-ray CT contrast measurements of GA-AuNP vectors for potential utility in molecular imaging. Our results demonstrate that naturally occurring GA can be used as a nontoxic phytochemical construct in the production of readily administrable biocompatible AuNPs for diagnostic and therapeutic applications in nanomedicine.
Strategies for production of active eukaryotic proteins in bacterial expression system (2012)

Bacteria have long been the favorite expression system for recombinant protein production. However, the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias, protein folding, phosphorylation, glycosylation, mRNA stability and promoter strength. Factors are cited and the methods to convert to soluble and active proteins are described, for example a tight control of Escherichia coli milieu, refolding from inclusion body and through fusion technology.
Experts launch action on acrylamide in staple foods (2002)

Food science experts and officials from Europe and the United States are taking concerted action to try to reduce potentially dangerous levels of acrylamide, a toxin known to cause cancer, in staple foods.

The possible health risk was discussed at a meeting convened by the World Health Organization in Geneva last month, after the announcement in March by Swedish scientists that acrylamides were present in a wide range of foods, particularly …
The thoracolumbar fascia: anatomy, function and clinical considerations (2012)

In this overview, new and existent material on the organization and composition of the thoracolumbar fascia (TLF) will be evaluated in respect to its anatomy, innervation biomechanics and clinical relevance. The integration of the passive connective tissues of the TLF and active muscular structures surrounding this structure are discussed, and the relevance of their mutual interactions in relation to low back and pelvic pain reviewed. The TLF is a girdling structure consisting of several aponeurotic and fascial layers that separates the paraspinal muscles from the muscles of the posterior abdominal wall. The superficial lamina of the posterior layer of the TLF (PLF) is dominated by the aponeuroses of the latissimus dorsi and the serratus posterior inferior. The deeper lamina of the PLF forms an encapsulating retinacular sheath around the paraspinal muscles. The middle layer of the TLF (MLF) appears to derive from an intermuscular septum that developmentally separates the epaxial from the hypaxial musculature. This septum forms during the fifth and sixth weeks of gestation. The paraspinal retinacular sheath (PRS) is in a key position to act as a 'hydraulic amplifier', assisting the paraspinal muscles in supporting the lumbosacral spine. This sheath forms a lumbar interfascial triangle (LIFT) with the MLF and PLF. Along the lateral border of the PRS, a raphe forms where the sheath meets the aponeurosis of the transversus abdominis. This lateral raphe is a thickened complex of dense connective tissue marked by the presence of the LIFT, and represents the junction of the hypaxial myofascial compartment (the abdominal muscles) with the paraspinal sheath of the epaxial muscles. The lateral raphe is in a position to distribute tension from the surrounding hypaxial and extremity muscles into the layers of the TLF. At the base of the lumbar spine all of the layers of the TLF fuse together into a thick composite that attaches firmly to the posterior superior iliac spine and the sacrotuberous ligament. This thoracolumbar composite (TLC) is in a position to assist in maintaining the integrity of the lower lumbar spine and the sacroiliac joint. The three-dimensional structure of the TLF and its caudally positioned composite will be analyzed in light of recent studies concerning the cellular organization of fascia, as well as its innervation. Finally, the concept of a TLC will be used to reassess biomechanical models of lumbopelvic stability, static posture and movement.
Physics of high-pressure helium and argon radio-frequency plasmas (2004)

The physics of helium and argon rf discharges have been investigated in the pressure range from 50 to 760Torr. The plasma source consists of metal electrodes that are perforated to allow the gas to flow through them. Current and voltage plots were obtained at different purity levels and it was found that trace impurities do not affect the shape of the curves. The electron temperature was calculated using an energy balance on the unbound electrons. It increased with decreasing pressure from 1.1 to 2.4eV for helium and from 1.1 to 2.0 for argon. The plasma density calculated at a constant current density of 138mA∕cm2 ranged from 1.7×1011 to 9.3×1011cm−3 for helium and from 2.5×1011 to 2.4×1012cm−3 for argon, increasing with the pressure. At atmospheric pressure, the electron density of the argon plasma is 2.5 times that of the helium plasma.
Direct detection of explosives on solid surfaces by mass spectrometry with an ambient ion source based on dielectric barrier discharge (2007)

Trace amounts of explosives on solid surfaces were detected by mass spectrometry at ambient conditions with a new technique termed dielectric barrier discharge ionization (DBDI). By the needle-plate discharge mode, a plasma discharge with energetic electrons was generated, which could launch the desorption and ionization of the explosives from solid surfaces. Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), 2,4,6-trinitrotoluene (TNT), and pentaerythritol tetranitrate (PETN) were desorbed directly from the explosives-contaminated surface by DBDI, forming the typical anions of [TNT](-), [TNT - H](-), [RDX + NO(2)](-), [PETN + ONO(2)](-), and [RDX + ONO(2)](-). The ions were transferred into the MS instrument for analysis in the negative ion mode. The detection limit of present method was 10 pg for TNT (m/z 197, S/N 8 : 1), 0.1 ng for RDX (m/z 284, S/N 10 : 1), and 1 ng for PETN (m/z 260, S/N 12 : 1). The present method allowed the detection of trace explosives on various matrices, including paper, cloth, chemical fiber, glass, paints, and soil. A relative standard deviation of 5.57% was achieved by depositing 100 pg of TNT on these matrices. The analysis of A-5, a mixture of RDX and additives, has been carried out and the results were consistent with the reference values. The DBDI-MS method represents a simple and rapid way for the detection of explosives with high sensitivity and specificity, which is especially useful when they are present in trace amounts on ordinary environmental surfaces.
Eliminating the hypervisor attack surface for a more secure cloud (2011)

Cloud computing is quickly becoming the platform of choice for many web services. Virtualization is the key underlying technology enabling cloud providers to host services for a large number of customers. Unfortunately, virtualization software is large, complex, and has a considerable attack surface. As such, it is prone to bugs and vulnerabilities that a malicious virtual machine (VM) can exploit to attack or obstruct other VMs -- a major concern for organizations wishing to move to the cloud. In contrast to previous work on hardening or minimizing the virtualization software, we eliminate the hypervisor attack surface by enabling the guest VMs to run natively on the underlying hardware while maintaining the ability to run multiple VMs concurrently. Our NoHype system embodies four key ideas: (i) pre-allocation of processor cores and memory resources, (ii) use of virtualized I/O devices, (iii) minor modifications to the guest OS to perform all system discovery during bootup, and (iv) avoiding indirection by bringing the guest virtual machine in more direct contact with the underlying hardware. Hence, no hypervisor is needed to allocate resources dynamically, emulate I/O devices, support system discovery after bootup, or map interrupts and other identifiers. NoHype capitalizes on the unique use model in cloud computing, where customers specify resource requirements ahead of time and providers offer a suite of guest OS kernels. Our system supports multiple tenants and capabilities commonly found in hosted cloud infrastructures. Our prototype utilizes Xen 4.0 to prepare the environment for guest VMs, and a slightly modified version of Linux 2.6 for the guest OS. Our evaluation with both SPEC and Apache benchmarks shows a roughly 1% performance gain when running applications on NoHype compared to running them on top of Xen 4.0. Our security analysis shows that, while there are some minor limitations with cur- rent commodity hardware, NoHype is a significant advance in the security of cloud computing.
Oscillatory Potential Analysis and ERGs of Normal and Diabetic Rats (2004)

OPs have been used to evaluate retinal function in both diabetic models and patients. The comparison of amplitude-matched OPs is a robust determinant of changes in kinetics. Researchers and clinicians who use OPs may wish to consider the relationship between OP amplitude and kinetics to avoid confounding assessments of abnormalities. The amplitude versus kinetics relationship does not change form in diabetic animals; it is merely shifted (delayed) on the time axis.
Caspase inhibition in camptothecin-treated U-937 cells is coupled with a shift from apoptosis to transient G1 arrest followed by necrotic cell death. (1999)

Leukemia U-937 cells rapidly undergo characteristic morphological changes, caspase activation, and DNA fragmentation typical of apoptosis on treatment with the DNA topoisomerase I inhibitor camptothecin (CPT). In a previous report (Sané, A. T., and Bertrand, R., Cancer Res., 58: 3066-3072, 1998), we showed that, after CPT treatment, caspase inhibition by the tripeptide derivative benzyloxycarbonyl-Val-Ala-Asp(Ome)-fluoromethyl ketone (zVAD-fmk) blocked apoptosis and slowed passage of the cells through S-G2 and caused a transient accumulation of these cells at the G1 phase of the cell cycle. Accumulation of these cells at G1 is not associated with major changes in expression level of cyclin-dependent kinase (cdk)2, cdk4, and cdk6; cyclin D1 and cyclin E; or p16, p21, p27, and p57 after CPT treatment. Furthermore, cdk2, cdk4, and cdk6 kinase activities remain unaffected after CPT treatment. These results indicate that the G1 arrest of these cells does not correlate with a classical driven cell cycle checkpoint but with the known effect of CPT in mediating inhibition of DNA replication and RNA transcription after stabilization of topoisomerase I-linked DNA strand breaks. However, persistent caspase inhibition after CPT treatment also results in cells falling into necrosis after the transient G1 arrest. These results indicate that the enforced inhibition of caspase activities does not confer a survival advantage upon CPT-treated cells but is coupled with a shift from apoptosis to transient G1 arrest followed by massive necrosis.
DAvinCi: A cloud computing framework for service robots (2010)

We propose DAvinCi, a software framework that provides the scalability and parallelism advantages of cloud computing for service robots in large environments. We have implemented such a system around the Hadoop cluster with ROS (Robotic Operating system) as the messaging framework for our robotic ecosystem. We explore the possibilities of parallelizing some of the robotics algorithms as Map/Reduce tasks in Hadoop. We implemented the FastSLAM algorithm in Map/Reduce and show how significant performance gains in execution times to build a map of a large area can be achieved with even a very small eight-node Hadoop cluster. The global map can later be shared with other robots introduced in the environment via a Software as a Service (SaaS) Model. This reduces the burden of exploration and map building for the new robot and minimizes it's need for additional sensors. Our primary goal is to develop a cloud computing environment which provides a compute cluster built with commodity hardware exposing a suite of robotic algorithms as a SaaS and share data co-operatively across the robotic ecosystem.
Positive and negative controls on cell growth (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPositive and negative controls on cell growthRobert A. WeinbergCite this: Biochemistry 1989, 28, 21, 8263–8269Publication Date (Print):October 17, 1989Publication History Published online1 May 2002Published inissue 17 October 1989https://pubs.acs.org/doi/10.1021/bi00447a001https://doi.org/10.1021/bi00447a001research-articleACS PublicationsRequest reuse permissionsArticle Views545Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Neisseria meningitidis encodes an FK506-inhibitable rotamase. (1992)

Eukaryotic peptidyl-prolyl cis-trans isomerases (rotamases) fall into two classes, the cyclophilins inhibited by cyclosporin A and the FK506-binding proteins inhibited by the macrolide antibiotic FK506. In prokaryotes homologs of cyclophilins have been identified and found to have rotamase activity. Sequence similarities have been noted between FK506-binding proteins and gene products in a number of bacterial species, but whether these bacterial proteins have rotamase activity is not known. Using the polymerase chain reaction, we have cloned and sequenced a homolog of an FK506-binding protein from Neisseria meningitidis and expressed the gene product as a fusion protein with maltose-binding protein. The fusion protein was purified by affinity chromatography. By measuring the rate of chymotrypsin cleavage of the substrate succinyl-Ala-Ala-Pro-Phe p-nitroanilide, we found that the fusion protein had rotamase activity comparable to that of human FK506-binding protein. This rotamase activity was inhibited by FK506.
Cell Cycle Regulation in Hematopoietic Stem/Progenitor Cells (2004)

Hematopoietic stem cells (HSCs) are characterized by pluripotentiality and a capacity for self-renewal. In order to both maintain a supply of mature blood cells and not to exhaust HSCs throughout the lifespan of the organism, most HSCs remain quiescent and only a limited number enter the cell cycle. In HSCs, the cell cycle is crucially regulated by external factors such as cytokines and interactions with stromal cells and the extracellular matrix (ECM) in the bone marrow (BM) microenvironment. In addition, intrinsic transcription factors expressed in HSCs, including c-Myb, GATA-2, HOX family proteins, and Bmi-1, also control their growth through their effect on gene transcription. In terms of the particular roles in regulation of the cell-cycle, p21WAF1 (p21) and p27KIP1 (p27) were shown to maintain the quiescence of HSCs and of progenitor cells, respectively, thereby governing their available pool sizes. Also, p16INK4A (p16) and p15INK4B (p15) are thought to act as tumor suppressors, since their inactivation and/or deletion are observable in various types of hematologic malignancies. These results make evident that appropriate cell cycle control, particularly at the early stage of stem/progenitor cells, is required for maintaining normal hematopoiesis.