gebhart/patrepeval huggingface dataset:
Robotic System to Evaluate Force Feedback in Minimally Invasive Computer Aided Surgery (2004)

We present an experimental system for robot assisted, minimally invasive surgery that is capable of force measurement and haptic feedback. While minimally invasive surgery with robots provides several advantages like reduced tissue trauma and shorter recovery times, there are also some inherent short-comings. Referring to surgeons, the most significant issue is the lack of haptic feedback. This often causes collateral trauma and leads to prolonged operation time. Therefore we have developed an open platform, which combines the advantages of present systems with the possibility of force reflection. We have included features known from commercial available systems, like full Cartesian control of the end effector, stereo vision and ergonomic input devices. We used the system to perform basic surgical tasks (like knot-tying) and to evaluate force feedback.
Overexpression of MAGE/GAGE genes in paclitaxel/doxorubicin-resistant human cancer cell lines. (2003)

Previous studies directed at identifying paclitaxel resistance genes in a paclitaxel-resistant subclone of the human ovarian cancer cell line SKOV-3 identified a novel cancer testis antigen, Taxol resistance-associated gene 3 (TRAG-3). Because investigation suggested that TRAG-3, located on chromosome Xq28, does not directly participate in the paclitaxel-resistant phenotype, it was hypothesized that TRAG-3 might be linked to a neighboring gene that is directly involved in the drug-resistant phenotype, or alternatively, overexpression of TRAG-3 might be attributable to coregulation with other cancer testis antigens. To distinguish between these two hypotheses, expression of the genes that flank TRAG-3 was evaluated, namely the Centrin 2 gene and several members of the MAGE gene cluster. Northern analysis demonstrates overexpression of MAGE2 but not Centrin 2. Extension of this analysis to other neighboring and non-neighboring representative cancer testis antigens reveals overexpression of MAGE3, MAGE6, MAGE11, and MAGE12, as well as GAGE-2, GAGE-4, GAGE-5, GAGE-6, and GAGE-7 (clustered on Xp11) in SKOV-3(TR), as compared with SKOV-3. In addition, Affymetrix-based analysis of gene expression in SKOV-3 subclones with variable paclitaxel resistance demonstrates MAGE gene overexpression occurs early in the development of the paclitaxel-resistant phenotype, whereas GAGE gene overexpression occurs somewhat later. Evaluation of additional breast and ovarian cancer cell lines reveals MAGE/GAGE overexpression in both paclitaxel- and doxorubicin-resistant cell lines, whereas gemcitabine-resistant subclones of several ovarian cancer cell lines, including SKOV-3(GR), reveals no change in MAGE/GAGE expression. To determine whether MAGE gene overexpression contributes directly to the drug-resistant phenotype, MAGE2 or MAGE6, cDNA was introduced into the paclitaxel-sensitive human ovarian cancer cell line OVCAR8. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxicity analysis of both MAGE2 and MAGE6 transfectants demonstrates a 4-fold increase in resistance to paclitaxel and 2-fold increase in resistance to doxorubicin but not to other drugs, such as topotecan and cisplatin, through a nonmultidrug resistance-1 mechanism. MAGE2 or MAGE6 overexpression also induces a growth advantage in OVCAR8-transfected cells. These studies suggest that the in vitro acquisition of paclitaxel and doxorubicin resistance can be associated with increased expression of a variety of both neighboring and non-neighboring cancer testis antigens genes. This does not appear to be a consequence of random genetic instability or genomic amplification of the X chromosome. These antigens, because of limited expression in normal tissues, may be suitable targets for immunotherapy and novel therapeutic strategies in the treatment of chemotherapy-resistant epithelial tumors.
Angiotensin II Receptor Blocker‐Associated Angioedema: On the Heels of ACE Inhibitor Angioedema (2002)

Angioedema and cough are known side effects of angiotensin-converting enzyme (ACE) inhibitors. Angiotensin-converting enzyme is a potent inhibitor of kinase II, which facilitates the breakdown of bradykinin. An increase in bradykinin levels results in continued prostaglandin E2 synthesis, vasodilation, increased vascular permeability, and increased interstitial fluid. In contrast, the angiotensin II receptor blockers (ARBs) do not increase bradykinin levels. Angioedema as a complication of ACE inhibitor therapy is not widely recognized; this complication is even less recognized with second-line ARBs. We report angioedema associated with losartan (an ARB) in a patient who had experienced angioedema secondary to enalapril (an ACE inhibitor). Almost half of patients with ARB-associated angioedema also had developed angioedema while receiving ACE inhibitor therapy. Clinicians should exercise caution when using ARBs in patients with a history of angioedema secondary to ACE inhibitors.
Gene Inactivation in the Cyanobacterium <I>Synechococcus </I>sp. PCC 7002 and the Green Sulfur Bacterium <I>Chlorobium tepidum</I> Using In Vitro-Made DNA Constructs and Natural Transformation (2004)

Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.
Plan for a Biography of Still (2000)

This chapter takes a look at a letter Forsythe wrote to Still, where the former requested some information for a proposed biography. The information Still sent to Forsythe can be found in the “Personal Notes” chapter. This chapter shows that Forsythe's letter included a proposed outline of the book. Forsythe planned to discuss Still in the first two sections, while the third and fourth sections were for an analysis of Negro Folk materials and pure African folk materials, and a discussion of the music itself.
STUDIES OF MOUSE POLYOMA VIRUS INFECTION (1959)

THREE PROCEDURES HAVE BEEN COMPARED FOR USEFULNESS IN TITRATION AND DETECTION OF POLYOMA VIRUS: production of cytopathic effect (CPE) in mouse embryo tissue culture, production of HI antibody after inoculation into weanling mice (MAP test), and production of tumors in suckling hamsters during a 3 to 5 week observation period. The tissue culture and mouse antibody production tests were generally comparable in sensitivity, reproducibility, and time required to obtain results. Titration by tumor production in suckling hamsters was not suitable for quantitation because of marked variation in susceptibility among animals. Virus was detected in tissues of normal mice from spontaneously infected colonies by either production of CPE in mouse embryo tissue culture or by the MAP test; virus was found in organs of 15 (58 per cent) of 26 mice with antibody, and 2 (8 per cent) of 24 mice without antibody.
RTM angle gathers using Poynting vectors (2011)

Angle‐domain common image gathers have been shown to provide several advantages for the analysis of migrated images. In this abstract we describe an efficient new method for creating angle gathers for reverse time migration (RTM). The method relies on the computation of the direction of energy flux (known in electromagnetics as the Poynting vector) in the space‐time domain. Given the Poynting vectors for source and receiver wavefields, we may readily derive reflection angle and azimuth, and reflector dip and azimuth if desired. Unlike some previously considered angle‐gather algorithms, the method depends only on local information at the reflection point, thereby avoiding the loss of resolution inherent in mixing quantities from different horizontal locations. In addition, we derive the weight function needed to produce correct relative amplitudes for these angle gathers.
Primary Open-Angle Glaucoma (2009)

Glaucoma is one of the leading causes of blindness worldwide. This review discusses the clinical features, genetics, molecular biology, and cell biology of glaucoma. The authors present a typical case of glaucoma, together with the ocular findings.
Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation (2009)

The use of muscle progenitor cells (MPCs) for regenerative medicine has been severely compromised by their decreased proliferative and differentiative capacity after being cultured in vitro. We hypothesized the loss of pivotal niche factors to be the cause. Therefore, we investigated the proliferative and differentiative response of passage 0 murine MPCs to varying substrate elasticities and protein coatings and found that proliferation was influenced only by elasticity, whereas differentiation was influenced by both elasticity and protein coating. A stiffness of 21 kPa optimally increased the proliferation of MPCs. Regarding differentiation, we demonstrated that fusion of MPCs into myotubes takes place regardless of elasticity. However, ongoing maturation with cross-striations and contractions occurred only on elasticities higher than 3 kPa. Furthermore, maturation was fastest on poly-d-lysine and laminin coatings.
Role of Histone Acetylation in Reprogramming of Somatic Nuclei Following Nuclear Transfer1 (2006)

Before fertilization, chromatins of both mouse oocytes and spermatozoa contain very few acetylated histones. Soon after fertilization, chromatins of both gametes become highly acetylated. The same deacetylation-reacetylation changes occur with histones of somatic nuclei transferred into enucleated oocytes. The significance of these events in somatic chromatin reprogramming to the totipotent state is not known. To investigate their importance in reprogramming, we injected cumulus cell nuclei into enucleated mouse oocytes and estimated the histone deacetylation dynamics with immunocytochemistry. Other reconstructed oocytes were cultured before and/or after activation in the presence of the highly potent histone deacetylase inhibitor trychostatin A (TSA) for up to 9 h postactivation. The potential of TSA-treated and untreated oocytes to develop to the blastocyst stage and to full term was compared. Global deacetylation of histones in the cumulus nuclei occurred between 1 and 3 h after injection. TSA inhibition of histone deacetylation did not affect the blastocyst rate (37% with and 34% without TSA treatment), whereas extension of the TSA treatment beyond the activation point significantly increased the blastocyst rate (up to 81% versus 40% without TSA treatment) and quality (on average, 59 versus 45 cells in day 4 blastocysts with and without TSA treatment, respectively). TSA treatment also slightly increased full-term development (from 0.8% to 2.8%). Thus, deacetylation of somatic histones is not important for reprogramming, and hyperacetylation might actually improve reprogramming.
SPICE modeling of microwave and RF control diodes (2002)

A SPICE model for the microwave and RF PIN switching diode is presented. The model simulates the important I-region charge storage phenomenon and its effect on the PIN diode impedance-frequency characteristic. The SPICE model is described and validated by comparisons with numerical, analytical and measured results.
TRAP-Array: A Disk Array Architecture Providing Timely Recovery to Any Point-in-time (2006)

RAID architectures have been used for more than two decades to recover data upon disk failures. Disk failure is just one of the many causes of damaged data. Data can be damaged by virus attacks, user errors, defective software/firmware, hardware faults, and site failures. The risk of these types of data damage is far greater than disk failure with today's mature disk technology and networked information services. It has therefore become increasingly important for today's disk array to be able to recover data to any point in time when such a failure occurs. This paper presents a new disk array architecture that provides timely recovery to any point-in-time, referred to as TRAP-array. TRAP-array stores not only the data stripe upon a write to the array, but also the time-stamped exclusive-ORs of successive writes to each data block. By leveraging the exclusive-OR operations that are performed upon each block write in today's RAID4/5 controllers, TRAP does not incur noticeable performance overhead. More importantly, TRAP is able to recover data very quickly to any point-in-time upon data damage by tracing back the sequence and history of exclusive-ORs resulting from writes. What is interesting is that TRAP architecture is amazingly space-efficient. We have implemented a prototype TRAP architecture using software at block device level and carried out extensive performance measurements using TPC-C benchmark running on Oracle and Postgress databases, TPC-W running on MySQL database, and file system benchmarks running on Linux and Windows systems. Our experiments demonstrated that TRAP is not only able to recover data to any point-in-time very quickly upon a failure but it also uses less storage space than traditional daily differential backup/snapshot. Compared to the state-of-the-art continuous data protection technologies, TRAP saves disk storage space by one to two orders of magnitude with a simple and a fast encoding algorithm. From an architecture point of view, TRAP-array opens up another dimension for storage arrays. It is orthogonal and complementary to RAID in the sense that RAID protects data in the dimension along an array of physical disks while TRAP protects data in the dimension along the time sequence
Development of Tumor-Infiltrating CD8+ T Cell Memory Precursor Effector Cells and Antimelanoma Memory Responses Are the Result of Vaccination and TGF-β Blockade during the Perioperative Period of Tumor Resection (2011)

A main goal of cancer immunology research is the formation of Ag-specific memory T cell immunity capable of activation upon tumor re-encounter. The requirements necessary to overcome the inhibitory signals present in the tumor microenvironment and form such memory T cell responses are unknown. In contrast to previous studies targeting tumors expressing highly immunogenic model Ags, we demonstrate that alleviating tumor-induced suppression along with vaccination against authentic Ags during the perioperative period provides long-lasting protection against a highly suppressive and poorly immunogenic melanoma. In this study, we employed DNA vaccination with an immunologically optimized mouse melanoma-shared Ag, Trp1ee/ng, combined with systemic TGF-β blockade during the perioperative period of primary tumor resection, to confer protection against B16 melanoma, and against JBRH, an independently derived melanoma unrelated to B16. Importantly, we demonstrate that correlative to memory responses, perioperative immunotherapy increases the formation of tumor-infiltrating and tumor-reactive CD8(+) T cells expressing low levels of the transcription factor T-bet, defined as memory precursor effector cells. We show that conditions for an immunologically fertile environment are met when TGF-β blockade and vaccination are applied during the perioperative period of primary tumor resection. These findings address limitations of current CD8(+) T cell immunotherapies against cancer by generating effective CD8(+) T cell memory recall responses.
Cocaine, Ethanol, and Genotype Effects on Human Midbrain Serotonin Transporter Binding Sites and mRNA Levels (1998)

Serotonin transporter binding sites were regulated in a region-specific and substance-specific pattern, which was not simply a local response to functional blockade. Also, a reciprocal relationship appeared to exist between cocaine and ethanol effects in the dorsal raphe, which may have interesting clinical implications for dual-diagnosis patients. It is possible that serotonin transporter promoter genotype may play a complex role in chronic ethanol dependence.
Priority encoding transmission (1996)

We introduce a new method, called priority encoding transmission, for sending messages over lossy packet-based networks. When a message is to be transmitted, the user specifies a priority value for each part of the message. Based on the priorities, the system encodes the message into packets for transmission and sends them to (possibly multiple) receivers. The priority value of each part of the message determines the fraction of encoding packets sufficient to recover that part. Thus even if some of the encoding packets are lost en-route, each receiver is still able to recover the parts of the message for which a sufficient fraction of the encoding packets are received. For any set of priorities for a message, we define a natural quantity called the girth of the priorities. We develop systems for implementing any given set of priorities such that the total length of the encoding packets is equal to the girth. On the other hand, we give an information-theoretic lower bound that shows that for any set of priorities the total length of the encoding packets must be at least the girth. Thus the system we introduce is optimal in terms of the total encoding length. This work has immediate applications to multimedia and high-speed networks applications, especially in those with bursty sources and multiple receivers with heterogeneous capabilities. Implementations of the system show promise of being practical.
Vaccination of Women with Metastatic Breast Cancer, Using a Costimulatory Gene (CD80)-Modified, HLA-A2-Matched, Allogeneic, Breast Cancer Cell Line: Clinical and Immunological Results (2003)

MDA-MB-231, an HLA-A2(+), HER2/neu(+) allogeneic breast cancer cell line genetically modified to express the costimulatory molecule CD80 (B7-1), was used to vaccinate 30 women with previously treated stage IV breast cancer. Expression of CD80 conferred the ability to deliver a costimulatory signal and thereby improved the antigen presentation capability of the tumor cells to patient T cells in vitro. Patients were vaccinated with 10(7) or 10(8) irradiated gene-modified tumor cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) or BCG, three times at 2-week intervals and then monthly until progressive disease developed. GM-CSF-related flulike symptoms and minor injection site reactions were observed frequently. Prolonged disease stabilization was observed in four patients but no objective tumor regressions were seen. Immune responses were measured in matched peripheral blood samples collected before and after treatment from 9 of 15 patients treated at the 10(8) tumor cell dose. Four patients exhibited MHC class I-restricted cytokine production in response to the parental breast cancer cell line. One patient maintained an increased number of circulating tumor-specific, interferon gamma-secreting CD8(+) T cells for 24 months after the last vaccination. One patient exhibited a tumor-specific interleukin 5 response to an autologous tumor cell line. This immunization strategy proved to be safe and feasible, and induced tumor-specific immune responses in a minority of patients; however, no objective tumor regressions were observed.
SCPD: a promoter database of the yeast Saccharomyces cerevisiae. (1999)

SCPD can be accessed from the URL http://cgsigma.cshl.org/jian. The database is continually updated.
The effects of dietary flavonoids on the regulation of redox inflammatory networks (2012)

Dietary flavonoids are a large family of polyphenols ubiquitously expressed in plants. Recent evidence show that flavonoids possess several anti-inflammatory activities due to their ability to scavenge reactive oxygen and nitrogen species (ROS and RNS), to inhibit the pro-inflammatory activity of ROS-generating enzymes including cyclooxygenase (COX), lipoxygenase (LOX) and inducible nitric oxide synthase (iNOS) and to modulate different intracellular signaling pathways from NF-kB to mitogen-activated protein kinases (MAPKs) through perturbation of redox-sensible networks in immune cells. This report will review current knowledge on the anti-inflammatory effects of flavonoids on immune cells focusing on their ability to modulate multiple redox-sensible pathways involved in inflammation.
Summary of measured radiofrequency electric and magnetic fields (10 kHz to 30 GHz) in the general and work environment (1997)

We have plotted data from a number of studies on the range of radiofrequency (RF) field levels associated with a variety of environmental and occupational sources. Field intensity is shown in units of volts/meter (V/m) for electric field strength and amps/meter (A/m) for magnetic field strength. Duty factors, modulation frequencies, and modulation indices are also reported for some sources. This paper is organized into seven sections, each cataloging sources into appropriate RF frequency bands from very-low frequency (VLF) to super-high frequency (SHF), and covers frequencies from 10 kHz to 30 GHz. Sources included in this summary are the following: Coast Guard navigational transmitters, a Navy VLF transmitter, computer visual display terminals (VDTs), induction stoves or range tops, industrial induction and dielectric heaters, radio and television broadcast transmitters, amateur and citizens band (CB) transmitters, medical diathermy and electrosurgical units, mobile and handheld transmitters, cordless and cellular telephones, microwave ovens, microwave terrestrial relay and satellite uplinks, and police, air traffic, and aircraft onboard radars. For the sources included in this summary, the strongest fields are found near industrial induction and dielectric heaters, and close to the radiating elements or transmitter leads of high power antenna systems. Handheld transmitters can produce near fields of about 500 V/m at the antenna. Fields in the general urban environment are principally associated with radio and TV broadcast services and measure about 0.1 V/m root-mean-square (rms). Peak fields from air traffic radars sampled in one urban environment were about 10 V/m, 300 times greater than the rms value of 0.03 V/m when the duty factor associated with antenna rotation and pulsing are factored in.
The Bundesbank and the making of an economic press story (2000)

The Bundesbank and the Making of an Economic Press Story