gebhart/patrepeval huggingface dataset:
Robotic assist devices for bimanual physical therapy: preliminary experiments (1993)

Preliminary experiments were performed on a dynamically simplified system consisting of two outstretched hands constrained to flexion/extension while holding an object. A prototype device was designed to measure and assist in a transport task, in which the two hands moved a pencil-like object rhythmically back and forth, and in a bimanual squeezing task, in which the two hands squeezed the object. The device, operating under simple proportional feedback control laws, could substitute completely for one hand in both tasks. When the device assisted a hand partially disabled by ischemia, it produced torque that the disabled hand did not, allowing the tasks to be performed. These results demonstrate the capability of a device, operating under simple control laws, to assist a disabled hand, allowing performance of coordinated bimanual tasks. Such a robot, extended to more useful bimanual activities of daily living, could afford cost effective, objectively measurable, self-driven therapy for a large patient population.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The role of ras gene mutations in myeloproliferative disorders. (1990)

Mutations in the ras genes frequently are found in myeloproliferative disorders. These abnormalities have prognostic significance in the myelodysplastic syndromes, but their clinical importance in other hematopathic states remains unclear. The study of this gene in leukemic conditions is providing insights into the genetic pathways leading to leukemic progression.
Segmentation and Clustering of Car-Following Behavior: Recognition of Driving Patterns (2014)

Driving behavior can be influenced by many factors that are not feasible to collect in driving behavior studies. The research presented in this paper investigates the characteristics of a wide range of driving behaviors linking driving states to the drivers' actions. The proposed methodology is structured such that a known state can be linked to multiple actions, thus accounting for the effects of unknown driving state factors. A two-step algorithm was developed and used for the segmentation and clustering of driving behaviors. The algorithm segments and clusters car-following behaviors based on eight state-action variables: the longitudinal acceleration, the lateral acceleration, the yaw rate, the vehicle speed, the lane offset, the yaw angle, the range, and the range rate. The results of this methodology are state-action clusters that define the driving pattern of drivers. The sample used in this paper included 20 different drivers, i.e., ten car and ten truck drivers. The results revealed that behavior patterns were different between car drivers but not between truck drivers. The results also show that car drivers exhibit behaviors that are unique to each driver, whereas truck drivers show a common driving pattern. The characteristics and frequency of recognized driving patterns are provided in this paper, along with the corresponding modeling parameters of each pattern.
A Novel Knowledge-Based System for Interpreting Complex Engineering Drawings: Theory, Representation, and Implementation (2009)

We present a novel knowledge-based system to automatically convert real-life engineering drawings to content-oriented high-level descriptions. The proposed method essentially turns the complex interpretation process into two parts: knowledge representation and knowledge-based interpretation. We propose a new hierarchical descriptor-based knowledge representation method to organize the various types of engineering objects and their complex high-level relations. The descriptors are defined using an Extended Backus Naur Form (EBNF), facilitating modification and maintenance. When interpreting a set of related engineering drawings, the knowledge-based interpretation system first constructs an EBNF-tree from the knowledge representation file, then searches for potential engineering objects guided by a depth-first order of the nodes in the EBNF-tree. Experimental results and comparisons with other interpretation systems demonstrate that our knowledge-based system is accurate and robust for high-level interpretation of complex real-life engineering projects.
Population and family planning policies: women-centred perspectives (1993)

(1993). Population and family planning policies: women-centred perspectives. Reproductive Health Matters: Vol. 1, Population and family planning policies, pp. 4-12.
Lung ischemia-reperfusion injury: a molecular and clinical view on a complex pathophysiological process (2010)

Lung ischemia-reperfusion injury remains one of the major complications after cardiac bypass surgery and lung transplantation. Due to its dual blood supply system and the availability of oxygen from alveolar ventilation, the pathogenetic mechanisms of ischemia-reperfusion injury in the lungs are more complicated than in other organs, where loss of blood flow automatically leads to hypoxia. In this review, an extensive overview is given of the molecular and cellular mechanisms that are involved in the pathogenesis of lung ischemia-reperfusion injury and the possible therapeutic strategies to reduce or prevent it. In addition, the roles of neutrophils, alveolar macrophages, cytokines, and chemokines, as well as the alterations in the cell-death related pathways, are described in detail.
Early processing of visual information (1976)

Abstract An introduction is given to a theory of early visual information processing. The theory has been implemented, and examples are given of images at various stages of analysis. It is argued that the first step of consequence is to compute a primitive but rich description of the grey-level changes present in an image. The description is expressed in a vocabulary of kinds of intensity change (EDGE, SHADING-EDGE, EXTENDED-EDGE, LINE, BLOB etc.). Modifying parameters are bound to the elements in the description, specifying their POSITION, ORIENTATION, TERMINATION points, CONTRAST, SIZE and FUZZINESS. This description is obtained from the intensity array by fixed techniques, and it is called the primal sketch. For most images, the primal sketch is large and unwieldy. The second important step in visual information processing is to group its contents in a way that is appropriate for later recognition. From our ability to interpret drawings with little semantic content, one may infer the presence in our perceptual equipment of symbolic processes that can define ‘ placetokens’ in an image in various ways, and can group them according to certain rules. Homomorphic techniques fail to account for many of these grouping phenomena, whose explanations require mechanisms of construction rather than mechanisms of detection. The necessary grouping of elements in the primal sketch may be achieved by a mechanism that has available the processes inferred from above, together with the ability to select items by first order discriminations acting on the elements’ parameters. Only occasionally do these mechanisms use downward-flowing information about the contents of the particular image being processed. It is argued that ‘non-attentive’ vision is in practice implemented by these grouping operations and first order discriminations acting on the primal sketch. The class of computations so obtained differs slightly from the class of second order operations on the intensity array. The extraction of a form from the primal sketch using these techniques amounts to the separation of figure from ground. It is concluded that most of the separation can be carried out by using techniques that do not depend upon the particular image in question. Therefore, figure-ground separation can normally precede the description of the shape of the extracted form. Up to this point, higher-level knowledge and purpose are brought to bear on only a few of the decisions taken during the processing. This relegates the widespread use of downward-flowing information to a later stage than is found in current machine-vision programs, and implies that such knowledge should influence the control of, rather than interfering with, the actual data-processing that is taking place lower down.
Salmonella entericaSerovar Typhimurium Strains with Regulated Delayed Attenuation In Vivo (2008)

Recombinant bacterial vaccines must be fully attenuated for animal or human hosts to avoid inducing disease symptoms while exhibiting a high degree of immunogenicity. Unfortunately, many well-studied means for attenuating Salmonella render strains more susceptible to host defense stresses encountered following oral vaccination than wild-type virulent strains and/or impair their ability to effectively colonize the gut-associated and internal lymphoid tissues. This thus impairs the ability of recombinant vaccines to serve as factories to produce recombinant antigens to induce the desired protective immunity. To address these problems, we designed strains that display features of wild-type virulent strains of Salmonella at the time of immunization to enable strains first to effectively colonize lymphoid tissues and then to exhibit a regulated delayed attenuation in vivo to preclude inducing disease symptoms. We recently described one means to achieve this based on a reversible smooth-rough synthesis of lipopolysaccharide O antigen. We report here a second means to achieve regulated delayed attenuation in vivo that is based on the substitution of a tightly regulated araC P(BAD) cassette for the promoters of the fur, crp, phoPQ, and rpoS genes such that expression of these genes is dependent on arabinose provided during growth. Thus, following colonization of lymphoid tissues, the Fur, Crp, PhoPQ, and/or RpoS proteins cease to be synthesized due to the absence of arabinose such that attenuation is gradually manifest in vivo to preclude induction of diseases symptoms. Means for achieving regulated delayed attenuation can be combined with other mutations, which together may yield safe efficacious recombinant attenuated Salmonella vaccines.
Speaking for the environment (1995)

Communicators have special opportunities to promote environmental ethics. This paper shows how environmental ethics relates to the workplace and gives ideas about how communicators can help the environment in their work.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Increase in bone mass after one year of percutaneous oestradiol and testosterone implants in post‐menopausal women who have previously received long‐term oral oestrogens (1992)

Subcutaneous oestradiol and testosterone implants will result in an increase in bone mass even after many years of oral oestrogen replacement therapy.
Nuclear Factor Kappa B: Important Transcription Factor and Therapeutic Target (1998)

Nuclear factor kappa B (NF-kappa B) is an ubiquitous rapid response transcription factor in cells involved in immune and inflammatory reactions, and exerts its effect by expressing cytokines, chemokines, cell adhesion molecules, growth factors, and immunoreceptors. In this manner, NF-kappa B contributes to immunologically mediated diseases such as allograft rejection, rheumatoid arthritis, and bronchial asthma. The prototypic inducible form of NF-kappa B is a heterodimer composed of NF-kB1 and RelA, which both belong to the NF-kappa B/Rel family of proteins. Inactive NF-kappa B is present in the cytoplasm complexed with an inhibitory protein, I kappa B. NF-kappa B is activated by a number of incoming signals from the cell surface. Released from I kappa B inhibition, NF-kappa B translocates into the nucleus and binds to the kappa B motif of the target gene. The NF-kappa B activation process can be inhibited by pharmacologic agents at each activation step. Glucocorticoids inhibit NF-kappa B by directly associating with NF-kappa B or by upregulating I kappa B expression. Cyclosporine and tacrolimus prevent NF-kappa B activation by inhibiting the action of calcineurin, a phosphatase that indirectly induces I kappa B degradation. Deoxyspergualin inhibits NF-kappa B by blocking its nuclear translocation. Aspirin and salicylates inhibit upstream events inducing I kappa B phosphorylation. Tepoxalin and antioxidants inhibit NF-kappa B activation by influencing the redox state of the cell. Further research is required to develop more specific inhibitors to treat diseases mediated by NF-kappa B.
INCORPORATION OF THE GUS GENE INTO ORCHIDS VIA EMBRYO ELECTROPHORESIS (1992)

The apical meristems of Calanthe orchid embryos were exposed to 1 mg/ml pBI-121 DNA in an electric field. pBI-121 contains the GUS marker gene glucoronidase under the control of the 35 S cauliflower mosaic virus promoter. A pipette containing 0.3% agarose and acetate buffer containing the DNA was placed on one end of the embryo; while the opposite end was in contact with a pipette containing only buffer and agarose. Uptake of the DNA into the meristem was monitored by 4′6-diamidino-2-phenylindole (DAPI) fluorescence. Optimal uptake occurred after 10 min of electrophoresis at 10 volts and 0.5 milliamps. Under these conditions, 55% of the embryos survived the treatment and 57% of those which survived were transformed as measured by GUS-positive staining. Leaves from 6 month old plants which developed from the transformed embryos expressed specific patterns of GUS staining.
Acquisition of high metastatic capacity after in vitro fusion of a nonmetastatic tumor line with a bone marrow-derived macrophage. (1984)

A low metastatic, thioguanine-resistant murine T lymphoma line (EbTGR) was hybridized in vitro, with the help of polyethylene glycol, with syngeneic bone marrow-derived macrophages. Two HAT-resistant hybrid lines (Eb-F1 and Eb-F2) were obtained from independent fusion cultures. A cytogenetic analysis revealed that most of the macrophage chromosomes except No. 12 had segregated or become rearranged 60 d after fusion, a time at which the cell lines had become stabilized in culture. Syngeneic mice inoculated subcutaneously with the tumor macrophage hybrid lines developed, very quickly, visceral metastases and died after less than 2 wk, while those inoculated with the parental line lived for greater than 6 wk and developed only localized, large primary tumors. The metastatic hybridomas expressed a similar tumor antigen as a spontaneous, in vivo derived, high metastatic variant (ESb) of the same tumor. This suggests that ESb cells might have arisen from a spontaneous fusion with a host macrophage.
Metaphase fluorescence in situ hybridization (FISH) in the follow‐up of 60 patients with haemkopietic malignancies (1994)

Summary. Metaphase DNA fluorescence in situ hybridization (metaphase‐FISH) was performed on follow‐up samples from 60 patients suffering from haemopoietic malignancies (acute and chronic myeloid leukaemia, acute lymphoblastic leukaemia, non‐Hodgkin's lymphoma and myelodysplastic syndorme). All patients had clonal chromosomal trisomies or translocations at diagnosis, and were treated by bone marrow transplantation (BMT), chemotherapy (CT) or interferon‐α therapy. Metaphase‐FISH was performed during therapy‐induced complete haematological remission (CR) (BMT and CT patients) using biotin‐labelled whole chromosme paint probes. 28% of all patients in CR were shown by FISH to have abnormal metaphase cells, and 62% of this group suffered a clinical relapse. Of those with negative FISH results (72%), 12% relapsed. In three CML patients treated with BMT a small population of t(9;22)‐positive cells was demonstrated. These cells disappeared during follow‐up without causing a relapse. One ALL patient had abnormal cells a short time after start of therapy but was also later found FISH‐negative. Furthermore, we demonstrated that metaphase‐FISH is a suitable method for quantifying the proportion of abnormal cells in CML patients during interferon‐α therapy. Metaphase‐FISH was also employed to detect a local replase in an ALL patient. Thus, metaphase‐FISH was found reliable and sensitive for detection of minimal residual disease in patients with haemopoietic malignancies.
Phosphines: Nucleophilic organic catalysts for the controlled ring‐opening polymerization of lactides (2002)

Abstract A new metal‐free synthetic approach to the controlled ring‐opening polymerization (ROP) of lactide with nucleophilic phosphines as transesterification catalysts is described. P(Bu) 3 , PhPMe 2 , Ph 2 PMe, PPh 3 , and related phosphines are commercially available, inexpensive catalysts that generate narrowly dispersed polylactides with predictable molecular weights. These organic catalysts must be used in combination with an initiator, such as an alcohol, to generate an alcoholate ester α‐end group upon ROP. A likely polymerization pathway is through a monomer‐activated mechanism, with minimal active species, facilitating narrow molecular weight distributions. © 2002 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 40: 844–851, 2002; DOI 10.1002/pola.10168
Postoperative adjuvant chemotherapy in non‐small cell lung cancer: Prognostic value of DNA ploidy and postrecurrent survival (1991)

Eighty-six patients with non-small cell lung cancer who underwent curative operations were postoperatively randomized to control and adjuvant chemotherapy groups. In the adjuvant chemotherapy group, patients received cisplatin-based combination chemotherapy 3 or 4 weeks after operation and the average cycle of chemotherapy was 2.3 (from 1 to 6 cycles). In this trial, no evidence of improved survival or delayed recurrence was seen in the treated patients. In multivariate analysis of prognostic variables, the most important factor was the pathological stage of the disease and, second, DNA ploidy of the primary tumor. Although histology (squamous vs. non-squamous cell carcinoma) had a trend to influence the survival, it was not a significant factor. A total of 33 patients had recurrences: 17 and 16 patients were in control and adjuvant chemotherapy groups, respectively. Postrecurrent survival in the adjuvant chemotherapy group was significantly shorter than that in the control group, as determined by the generalized Wilcoxon and log rank tests. Median survival time after recurrence in the control and adjuvant therapy groups was 18.5 and 7.5 months, respectively. These results suggest that DNA ploidy of primary tumors should be considered as a prognostic factor in future trials of adjuvant therapy. Furthermore, analysis of postrecurrent survival in the adjuvant chemotherapy trial, as well as that of overall and disease-free survivals should be done.
Tool support for transformation from an OWL ontology to an HLA Object Model (2010)

Designing simulation architectures based on domain models is a promising approach. Tools to support transformation of formalized domain models to design models are essential. Ontology languages offer a way of formally specifying the domain knowledge. We adopt a user-guided approach to model transfor
In vitro assembly properties of vimentin mutagenized at the-site tail motif (1993)

The intermediate filament (IF) proteins vimentin, desmin and peripherin share a 9-residue sequence motif (beta-site) located near the end of their COOH-terminal tail domain. Peptide inhibition experiments have previously suggested that the beta-site is involved in interactions that limit the lateral growth of IFs and prevent inappropriate filament-filament associations. To investigate this question further, we have constructed and expressed, in Escherichia coli, hamster vimentin bearing different mutations in the beta-site. We show here that a single exchange of glycine 450 with a valine residue, or an internal deletion of amino acids 444-452, strongly interferes with the normal assembly of IFs under in vitro conditions. These mutants polymerize into irregular fibrils that have a strong tendency to anastomose and laterally aggregate under isotonic conditions. In contrast, a non-conservative substitution of arginine 448 for glutamic acid does not significantly interfere with filament structure and yields subunits that polymerize into long, smooth filaments that show a slight aberration in thickness. All mutant proteins are soluble in low salt and form oligomers similar to the ones formed by wild-type vimentin. On the basis of these findings and on related observations, we propose that the tail domain of type III IF proteins contains important structural elements involved in lateral protofilament-protofilament interactions.
VEGF Trap as a Novel Antiangiogenic Treatment Currently in Clinical Trials for Cancer and Eye Diseases, and VelociGene(R)- based Discovery of the Next Generation of Angiogenesis Targets (2005)

The concept that tumors can be controlled by directly targeting their vascular supply has finally come of age, because clinical trials using a humanized monoclonal antibody that blocks VEGF have demonstrated exciting efficacy in cancer patients, as well as in vascular eye diseases that can lead to blindness. However, data suggest that these current regimens may not provide complete VEGF inhibition and, thus, that the maximum therapeutic potential of VEGF blockade has not yet been achieved. We describe the status of a very potent and high-affinity VEGF blocker, termed the VEGF Trap, that may provide the opportunity to maximize the potential of VEGF blockade in cancer as well as in vascular eye diseases. We also describe use of the VEGF Trap as a research tool, when coupled to high-throughput mouse genetics approaches such as VelociGene that can be exploited in strategies to discover and validate the next generation of angiogenesis targets.
Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro (2009)

Pluripotent epiblast stem cells (EpiSCs) derived from postimplantation embryos exhibit properties that are characteristically different when compared with pluripotent embryonic stem cells (ESCs) derived from mouse blastocysts. However, EpiSCs are relatively less well characterised compared with ESCs. In particular, the relationship between EpiSCs and primordial germ cells (PGCs) is unknown, and is worthy of investigation because PGCs originate from postimplantation epiblast cells in vivo. We show that EpiSCs have an infinite capacity for generating PGCs, under conditions that sustain their pluripotency and self-renewal. These PGCs generated in vitro show appropriate transcriptional and epigenetic reprogramming events and are able to develop further into late germ cells. Notably, the PGCs can, in turn, be induced to undergo dedifferentiation into pluripotent embryonic germ cells (EGCs), which resemble ESCs and not the EpiSC from which they are derived. Our observations demonstrate intrinsic reprogramming during specification of PGCs that results in the erasure of epigenetic memory of EpiSCs following reactivation of the X-chromosome, DNA demethylation and re-expression of key pluripotency genes. This study provides novel insights into the nature and properties of EpiSCs, and introduces an in vitro model system that will be useful for investigations on PGC specification and on mechanisms regulating epigenetic reprogramming in germ cells.