gebhart/patrepeval huggingface dataset:
Proteolytic processing of tau (2010)

Tau aggregation is a hallmark of several neurodegenerative diseases, including AD (Alzheimer's disease), although the mechanism underlying tau aggregation remains unclear. Recent studies show that the proteolysis of tau plays an important role in both tau aggregation and neurodegeneration. On one hand, truncation of tau may generate amyloidogenic tau fragments that initiate the aggregation of tau, which in turn can cause toxicity. On the other hand, truncation of tau may result in tau fragments which induce neurodegeneration through unknown mechanisms, independently of tau aggregation. Blocking the truncation of tau thus may represent a promising therapeutic approach for AD or other tauopathies. In the present paper, we summarize our data on tau cleavage in a cell model of tauopathy and major results on tau cleavage reported in the literature.
Steady-State, ac-Temperature Calorimetry (1968)

A steady-state technique for measuring heat capacity using ac heating is described. Heat is applied sinusoidally in time to a sample coupled thermally to a reservoir; the resultant equilibrium temperature of the sample contains a term that is both inversely proportional to the heat capacity and measurable with high precision. The effects of various corrections that must be applied to the data are considered in detail. Measurements of the absolute magnitude of the heat capacity of indium and the field dependence of the heat capacity of beryllium have been made and are used to illustrate the power of the method. The observed quantum oscillations in the heat capacity of beryllium are in agreement with predictions based on other measurements.
The neonatal Fc receptor is expressed by human lymphocytes (2010)

The neonatal Fc receptor (FcRn) is an MHC class I like receptor that binds immunoglobulin G (IgG) in acidic environment (mainly endosomes). The FcRn protects IgG from lysosomal degradation, controls its transport between cell layers and extends IgG serum half-life [1]. It has been shown that the FcRn is expressed by many human cells including APCs and monocytes [2]. Thus far FcRn expression by human lymphocytes has not been proven.
Purification and Characterization of Two Lectins from Aloe arborescens Mill (1979)

Two lectins have been isolated from leaves of Aloe arborescens Mill by salt precipitation, pH-dependent fractionation and gel filtration. One lectin (P-2) has a molecular weight of approximately 18,000, consists of two subunits (alphabeta) and contains more than 18% by weight of neutral carbohydrate. The smaller subunit (alpha) has a molecular weight of approximately 7,500 and the larger subunit (beta) a molecular weight of approximately 10,500. The other lectin (S-1) has a molecular weight of approximately 24,000, consists of two subunits (gamma2) with a molecular weight of approximately 12,000 and contains more than 50% by weight of neutral carbohydrate. An interesting feature of the amino acid compositions of these lectins is the high proportion of acidic amino acids, such as aspartic acid and glutamic acid, and the low proportion of methionine and histidine. S-1 has a strong hemagglutinating activity. On the other hand, P-2 has not only hemagglutinating activity but also mitogenic activity on lymphocytes, precipitate-forming reactivity with serum proteins, one of which is alpha2-macroglobulin, and complement C3 activating activity via the alternate pathway.
Over One Hundred Peptide-Activated G Protein-Coupled Receptors Recognize Ligands with Turn Structure (2005)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOver One Hundred Peptide-Activated G Protein-Coupled Receptors Recognize Ligands with Turn StructureJoel D. A. Tyndall, Bernhard Pfeiffer, Giovanni Abbenante, and David P. FairlieView Author Information Center for Drug Design and Development, Institute for Molecular Bioscience, University of Queensland, Brisbane, Qld 4072, Australia Cite this: Chem. Rev. 2005, 105, 3, 793–826Publication Date (Web):February 22, 2005Publication History Received23 August 2004Published online22 February 2005Published inissue 1 March 2005https://pubs.acs.org/doi/10.1021/cr040689ghttps://doi.org/10.1021/cr040689gresearch-articleACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views2606Altmetric-Citations192LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chemical structure,Conformation,Ligands,Peptides and proteins,Receptors Get e-Alerts
Homogeneous and inhomogeneous Jones matrices (1994)

The classification of polarization properties of polarization elements is studied to derive data-reduction equations for extracting the diattenuation, retardance, and other polarization properties from their Jones matrices. Polarization elements, and Jones matrices as well, are divided into two classes: homogeneous, with orthogonal eigenpolarizations, and inhomogeneous, with nonorthogonal eigenpolarizations. The basic polarization properties, diattenuation and retardance, of homogeneous polarization elements are straightforward and well known; these elements are characterized by their eigenvalues and eigenpolarizations. Polarization properties of inhomogeneous polarization elements are not so evident. By applying polar decomposition, the definitions of diattenuation and retardance are generalized to inhomogeneous polarization elements, providing an understanding of their polarization characteristics. Furthermore, an inhomogeneity parameter is introduced to describe the degree of inhomogeneity in a polarization element. These results are then adapted to degenerate polarization elements, which have only one linearly independent eigenpolarization.
Optimizing ETL Processes in Data Warehouses (2005)

Extraction-transformation-loading (ETL) tools are pieces of software responsible for the extraction of data from several sources, their cleansing, customization and insertion into a data warehouse. Usually, these processes must be completed in a certain time window; thus, it is necessary to optimize their execution time. In this paper, we delve into the logical optimization of ETL processes, modeling it as a state-space search problem. We consider each ETL workflow as a state and fabricate the state space through a set of correct state transitions. Moreover, we provide algorithms towards the minimization of the execution cost of an ETL workflow.
Glossary of biotechnology and genetic engineering. (1999)

The Glossary of biotechnology and genetic engineering outlines the scientific terms that are mainly utilized in those areas and their definitions. It does not try to cover all areas of genetics and biochemistry, as those have already been covered in more specialized glossaries, but it does bring up to date the terms more frequently used by writers and workers on modern biotechnology and genetic engineering. It includes terms used in genetics, biochemistry, physiology, tissue culture, immunology and animal reproduction.
Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates and Antibody Mimetics (2018)

Strategies for site-specific modification of proteins have increased in number, complexity, and specificity over the last years. Such modifications hold the promise to broaden the use of existing biopharmaceuticals or to tailor novel proteins for therapeutic or diagnostic applications. The recent quest for next-generation antibody-drug conjugates (ADCs) sparked research into techniques with site selectivity. While purely chemical approaches often impede control of dosage or locus of derivatization, naturally occurring enzymes and proteins bear the ability of co- or post-translational protein modifications at particular residues, thus enabling unique coupling reactions or protein fusions. This review provides a general overview and focuses on chemo-enzymatic methods including enzymes such as formylglycine-generating enzyme, sortase, and transglutaminase. Applications for the conjugation of antibodies and antibody mimetics are reported.
Improving independent component analysis performances by variable selection (2004)

Blind source separation (BSS) consists in recovering unobserved signals from observed mixtures of them. In most cases the whole set of mixtures is used for the separation, possibly after a dimension reduction by PCA. This paper aims to show that in many applications the quality of the separation can be improved by first selecting a subset of some mixtures among the available ones, possibly by an information content criterion, and performing PCA and BSS afterwards. The benefit of this procedure is shown on simulated electrocardiographic data by extracting the fetal electrocardiogram signal from mixtures recorded on the abdomen of a pregnant woman.
Identification and Strain Differentiation of Vibrio cholerae by Using Polyclonal Antibodies against Outer Membrane Proteins (2001)

Cholera is caused only by O1 and O139 Vibrio cholerae strains. For diagnosis, 3 working days are needed for bacterial isolation from human feces and for biochemical characterization. Here we describe the purification of bacterial outer membrane proteins (OMP) from V. cholerae O1 Ogawa, O1 Inaba, and O139 strains, as well as the production of specific antisera and their use for fecal Vibrio antigen detection. Anti-OMP antisera showed very high reactivity and specificity by enzyme-linked immunosorbent assay (ELISA) and dot-ELISA. An inmunodiagnostic assay for V. cholerae detection was developed; this assay avoids preenrichment and costly equipment and can be used for epidemiological surveillance and clinical diagnosis of cases, considering that prompt and specific identification of bacteria is mandatory in cholera.
Region of peptide 125-147 of acetylcholine receptor alpha subunit is exposed at neuromuscular junction and induces experimental autoimmune myasthenia gravis, T-cell immunity, and modulating autoantibodies. (1985)

A major antigenic region of native nicotinic acetylcholine receptors (AcChoR) has been identified by using a synthetic disulfide-looped peptide corresponding to alpha-subunit residues 125-147 of Torpedo electric organ AcChoR: Lys-Ser-Tyr-Cys-Glu-Ile-Ile-Val-Thr-His-Phe- Pro-Phe-Asp-Gln-Gln-Asn-Cys-Thr-Met-Lys-Leu-Gly. The peptide bound 26-56% of polyclonal antibodies induced in rat, rabbit, and dog by immunization with native AcChoR. Rats inoculated with 50 micrograms of unconjugated peptide developed helper T-cell responses, delayed hypersensitivity, and antibodies to native AcChoR. Anti-peptide antibodies were more reactive with native than denatured AcChoR and bound to the alpha subunit. Some reacted exclusively with mammalian muscle AcChoR, some induced modulation of AcChoR on cultured myotubes, but none inhibited binding of alpha-bungarotoxin to solubilized or membrane-associated AcChoR. Repeated immunization induced experimental autoimmune myasthenia gravis: clinical signs in one rat and electrophysiologic and/or biochemical signs in 10 of 11 rats. Thus, at least part of the corresponding region of the mammalian AcChoR alpha subunit is extracellular at the neuromuscular junction and a potential target for pathogenic autoantibodies in patients with acquired myasthenia gravis.
Discharge planning and follow-up care: the asphyxiated infant. (1991)

Discharge planning and follow-up care of the asphyxiated infant is a complex process. Models of discharge planning, team member responsibilities, and teaching responsibilities are components of hospital discharge plans. Special care needs of these infants may include vision, hearing, immunizations, seizures, medications, and feeding. Families and health care professionals need to be familiar with programs providing financial resources for care of the infant such as private insurance, prepaid health care, Medicaid, Medical Needy program, Children with Special Health Care Needs (CSHCN), federal legislation mandating education and services for high-risk infants (PL 99-142 and PL 99-457) and intervention programs. Families returning to Newborn Follow-up programs become acquainted with a variety of professionals and types of neonatal and infant assessments. Providing teaching materials and information regarding special health problems, services and outcome, as it becomes known, is the responsibility of the extended health care team of nurses, physicians, home health services, psychologists, and therapists.
De NovoDesign of Helical Bundles as Models for Understanding Protein Folding and Function (2000)

De novo protein design has proven to be a powerful tool for understanding protein folding, structure, and function. In this Account, we highlight aspects of our research on the design of dimeric, four-helix bundles. Dimeric, four-helix bundles are found throughout nature, and the history of their design in our laboratory illustrates our hierarchic approach to protein design. This approach has been successfully applied to create a completely native-like protein. Structural and mutational analysis allowed us to explore the determinants of native protein structure. These determinants were then applied to the design of a dinuclear metal-binding protein that can now serve as a model for this important class of proteins.
A Technical Introduction to Digital Video (1996)

Video Monitor Adjustments: Black Level and Picture Gamma Component Video Colour Coding Composite NTSC and PAL Colour. Appendices: Gamma Correction on the Apple Macintosh Reducing Eyestrain.
ChemInform Abstract: Chemical Modification of Hyaluronic Acid for Drug Delivery, Biomaterials and Biochemical Probes (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Role of MD-2 in TLR2- and TLR4-mediated recognition of Gram-negative and Gram-positive bacteria and activation of chemokine genes (2000)

MD-2 is associated with TLR4 on the cell surface and enables TLR4 to respond to LPS. TLR2 without MD-2 does not respond to pure protein-free endotoxic LPS, ReLPS, and lipid A. MD-2 enables TLR2 to respond to non-activating LPS, ReLPS, and lipid A, and enhances TLR2-mediated responses to Gram-negative and Gram-positive bacteria, protein-containing LPS, peptidoglycan, and lipoteichoic acid. MD-2 enables TLR4 to respond to a wide variety of endotoxic LPS partial structures, Gram-negative bacteria, and Gram-positive lipoteichoic acid, but not to Gram-positive bacteria, peptidoglycan, and lipopeptide. MD-2 physically associates with both TLR4 and TLR2, but the association with TLR2 is weaker than with TLR4. Also, MD-2 and TLR2 and TLR4 enhance each other's expression. The highest induced genes in human monocytes stimulated with Gram-positive and Gram-negative bacterial cell wall components are chemokine genes, and IL-8 is the highest induced chemokine. Both Gram-positive and Gram-negative bacteria activate TLR2-->MyD88-->IRAK-->TRAF-->NIK-->IKK-->NF-->kappaB signal transduction pathway that induces transcription of the IL-8 gene. Therefore, TLR2 is a functional receptor for both Gram-positive and Gram-negative bacteria and it induces activation of IL-8.
Hormesis: A Highly Generalizable and Reproducible Phenomenon With Important Implications for Risk Assessment (1999)

From a comprehensive search of the literature, the hormesis phenomenon was found to occur over a wide range of chemicals, taxonomic groups, and endpoints. By use of computer searches and extensive cross‐referencing, nearly 3000 potentially relevant articles were identified. Evidence of chemical and radiation hormesis was judged to have occurred in approximately 10oO of these by use of a priori criteria. These criteria included study design features (e.g., number of doses, dose range), dose‐response relationship, statistical analysis, and reproducibility of results. Numerous biological endpoints were assessed, with growth responses the most prevalent, followed by metabolic effects, reproductive responses, longevity, and cancer. Hormetic responses were generally observed to be of limited magnitude with an average maximum stimulation of 30 to 60 percent over that of the controls. This maximum usually occurred 4‐ to 5‐fold below the NOAEL for a particular endpoint. The present analysis suggests that hormesis is a reproducible and generalizable biological phenomenon and is a fundamental component of many, if not most, dose‐response relationships. The relatively infrequent observation of homesis in the literature is believed to be due primarily to experimental design considerations, especially with respect to the number and range of doses and endpoint selection. Because of regulatory considerations, most toxicologic studies have been carried out at high doses above the low‐dose region where the hormesis phenomenon occurs.
Translational control of human p53 expression in yeast mediated by 5'-UTR-ORF structural interaction (2001)

We have expressed human p53 cDNA in the yeast Saccharomyces cerevisiae and shown that the level of production and the length of the p53 protein depends on the presence of untranslated mRNA regions (UTRs). The expression of the ORF alone leads to a p53 protein of correct size (53 kDa) that accumulates to high levels, concomitantly with the presence of a small amount of a p40 protein (40 kDa). However, when either the entire 5'-UTR and a part of the 3'- or 5'-UTR alone is used, this leads to the production of small amounts of the 40 kDa truncated form only. The p40 protein corresponds to a truncated form of p53 at the C-terminal extremity since it reacts only with a monoclonal antibody recognising the N-terminal epitope. This effect on the amount and length of p53 protein had no correlation at the mRNA level, suggesting that translational control probably occurs through the 5'-UTR. We propose a model of structural interaction between this UTR and a part of the ORF mRNA for the regulation of p53 expression in this heterologous context.
DRAM based Intrinsic Physical Unclonable Functions for System Level Security (2015)

Physical Unclonable Functions (PUF) are the result of random uncontrollable variables in the manufacturing process. A PUF can be used as a source of random but reliable data for applications such as generating chip identification and encryption keys. Among various types of PUFs, an intrinsic PUF is the result of a preexisting manufacturing process, does not require any additional circuitry, and is cost effective. In this paper, we introduce an intrinsic PUF based on dynamic random access memories (DRAM). DRAM PUFs can be used in low cost identification applications and also have several advantages over other PUFs such as large input patterns. The DRAM PUF relies on the fact that the capacitor in the DRAM initializes to random values at startup. We demonstrate real DRAM PUFs and describe an experimental setup to test different operating conditions on three DRAMs to achieve the highest reliable results. Finally, we select the most stable bits to use as chip ID using our enrollment algorithm.
HLA class I expression and chromosomal deletions at 6p and 15q in head and neck squamous cell carcinomas (1999)

Loss at the chromosomal region 6p21.3 is a frequent event in head and neck squamous cell carcinomas (HNSCC). Since the human leukocyte antigen (HLA) complex is located at 6p21.3, loss of heterozygosity (LOH) of this region may provide tumour cells with an immune-escape tumour phenotype. In the present study, we have studied the correlation of HLA class I, TAP1 and TAP2 expression and LOH at 6p21.3. HLA class I and TAP1 and TAP2 protein expression was analysed by immunohistochemical procedures. A panel of 41 HNSCC with downregulated HLA class I expression was selected for LOH studies using 5 microsatellite markers located at 6p21.3 (D6S105, D6S265, D6S276, D6S273, D6S291) and 2 markers located at the chromosome 6 centromere (D6S473) and the 6p telomere (D6S277). In addition, LOH of the beta-2-nmicroglobulin (beta2m) gene was studied using 2 microsatellite markers flanking the beta2m gene (D15S126 and D15S153) and was correlated with beta2m and HLA class I expression. In 20/41 (49%) of the HNSCC, allelic loss for at least one locus at 6p21.3 was found. Loss at 15q was found in 4/10 (40%) HNSCC with downregulated beta2m expression and in 12/41 (29%) HNSCC with downregulated HLA class I expression. Our data show that downregulation of HLA class I expression is correlated with loss of chromosomal regions at 6p21.3 in HNSCC. In addition, LOH at 6p21.3 and 15q in 10 paired samples of DNA derived from the primary HNSCC, the lymph node metastases and from peripheral blood lymphocytes (PBLs) was studied. Five (5/10) primary tumours contained the same deletion as the corresponding lymph node metastases. The other cases contained deletions either in the primary tumour (3 cases) or in the lymph node metastases (1 case) or no deletions at all (1 case).
Volatile Constituents of the Fruits ofMuntingia calaburaL. (1996)

ABSTRACT The volatile constituents of ripe fruits of Muntingia calabura L. were analyzed by capillary GC and GC/MS following isolation by vacuum distillation-extraction and steam distillation-extraction. A total of 42 compounds were identified in the vacuum distillation extract which was dominated by alcohols (44.7%), esters (26.5%) and carbonyl compounds (23.3%). Steam distillation-extraction resulted in the identification of 56 compounds, among which esters (31.4%), alcohols (15.9%), phenolic compounds (11.3%), sesquiterpenoids (10.6%) and furan derivatives (8.3%) were quantitatively significant. A potent odorant detected was 2-acetyl-l-pyrroline (1.3%). In both isolates methyl salicylate was the most abundant component.
Catheter cryoablation of cardiac arrhythmias. (2005)

For many cardiac arrhythmias, catheter cryoablation is a safe and effective alternative to radiofrequency ablation. The ability to identify suitable ablation targets by reversible cryomapping is a particularly useful feature of this technology. Although the acute procedural success rate of cryoablation may not equal that of radiofrequency ablation in all circumstances, as catheter technology evolves it is likely that the efficacy of cryoablation will improve and the list of arrhythmias that can be treated with this method will expand.