gebhart/patrepeval huggingface dataset:
PDLIM4 repression by hypermethylation as a potential biomarker for prostate cancer. (2006)

Our analysis revealed differential expression of eight down-regulated and three up-regulated genes, implicating their role in prostate cancer development and progression. We further showed that the hypermethylation of PDLIM4 gene could be used as a sensitive molecular tool in detection of prostate tumorigenesis.
CD44 on LS174T Colon Carcinoma Cells Possesses E-Selectin Ligand Activity (2005)

Metastasis of circulating tumor cells requires a multistep cascade of events initiated by adhesion of tumor cells to the vascular endothelium of involved tissues. This process occurs under the forces of blood flow and is promoted by adhesion molecules specialized to interact under shear conditions. The endothelial molecule E-selectin is a major mediator of these adhesive events, and there is strong evidence that E-selectin receptor-ligand interactions contribute to the formation of metastasis. However, little is known about the identity of E-selectin ligand(s) expressed on cancer cells. To address this issue, we did SDS-PAGE analysis of membrane proteins, metabolic inhibition studies, and blot rolling assays of LS174T, a colon carcinoma cell line known to interact with E-selectin under physiologic flow conditions. Our studies show that LS174T cells express the hematopoietic cell E/L-selectin (HCELL) glycoform of CD44, which functions as a high-affinity E-selectin glycoprotein ligand on these cells. However, in contrast to the HCELL glycoform on human hematopoietic progenitor cells, which expresses carbohydrate-binding determinant(s) for E-selectin primarily on N-glycans of standard CD44, the relevant determinant(s) on LS174T cells is expressed on O-glycans and is predominantly found on variant isoforms of CD44 (CD44v). Our finding that tumor-associated CD44 splice variant(s) express E-selectin ligand activity provides novel perspectives on the biology of CD44 in cancer metastasis.
A new multiharmonic loading method for large-signal microwave and millimeter-wave transistor characterization (1991)

A multiharmonic loading method for nonlinear microwave and millimeter-wave transistor characterization using six-port techniques is presented. The system allows independent load tuning of an excitation signal and its harmonics. Load-pull measurements on a MESFET which have been performed at the fundamental frequency f/sub 0/ and at the second (2f/sub 0/) and third (3f/sub 0/) harmonics are discussed. The results highlight the importance of such measurement in designing and modeling nonlinear devices and circuits. The experimental results are found to be directly applicable for optimizing efficiency and output power in high-power MESFET amplifiers and MESFET frequency multipliers.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
3D printed metal columns for capillary liquid chromatography (2014)

Coiled planar capillary chromatography columns (0.9 mm I.D. × 60 cm L) were 3D printed in stainless steel (316L), and titanium (Ti-6Al-4V) alloys (external dimensions of ~5 × 30 × 58 mm), and either slurry packed with various sized reversed-phase octadecylsilica particles, or filled with an in situ prepared methacrylate based monolith. Coiled printed columns were coupled directly with 30 × 30 mm Peltier thermoelectric direct contact heater/cooler modules. Preliminary results show the potential of using such 3D printed columns in future portable chromatographic devices.
Multicomponent Patterning of Layer‐by‐Layer Assembled Polyelectrolyte/Nanoparticle Composite Thin Films with Controlled Alignment (2005)

Composite thin films of polyelectrolytes and fluorescent nanoparticles can be directly transfer-printed onto various substrates including indium tin oxide coated poly(ethylene terephthalate). The sequential transfer printing of thin films with controlled alignment introduces multicomponent patterns onto substrates, demonstrating possible practical device fabrication using functional polyelectrolyte multilayer composite thin films (see Figure).
Fulfilling the pedestrian protection directive using a long-wavelength infrared camera designed to meet both performance and cost targets (2006)

Pedestrian fatalities are around 15% of the traffic fatalities in Europe. A proposed EU regulation requires the automotive industry to develop technologies that will substantially decrease the risk for Vulnerable Road Users when hit by a vehicle. Automatic Brake Assist systems, activated by a suitable sensor, will reduce the speed of the vehicle before the impact, independent of any driver interaction. Long Wavelength Infrared technology is an ideal candidate for such sensors, but requires a significant cost reduction. The target necessary for automotive serial applications are well below the cost of systems available today. Uncooled bolometer arrays are the most mature technology for Long Wave Infrared with low-cost potential. Analyses show that sensor size and production yield along with vacuum packaging and the optical components are the main cost drivers. A project has been started to design a new Long Wave Infrared system with a ten times cost reduction potential, optimized for the pedestrian protection requirement. It will take advantage of the progress in Micro Electro-Mechanical Systems and Long Wave Infrared optics to keep the cost down. Deployable and pre-impact braking systems can become effective alternatives to passive impact protection systems solutions fulfilling the EU pedestrian protection regulation. Low-cost Long Wave Infrared sensors will be an important enabler to make such systems cost competitive, allowing high market penetration.
Performance of feedback and switch space diversity 900 MHz FM mobile radio systems with Rayleigh fading (1973)

A theoretical and experimental comparison of performance has been made between two types of predetection switching space diversity mobile radio systems. This comparison was made at a frequency of 840 MHz using simulated Rayleigh fading for a vehicle speed of about 80 mi/h. The switch diversity system was a conventional receiver antenna switching technique with two simulated physically separated receiving antennas and a single transmitting antenna. The feedback diversity system used a single receiving antenna with two simulated physically separated transmitting antennas. The transmitting antennas were switched remotely from the receiver. The difference in the performance of the two systems was shown to be primarily due to time delay inherent in the remote antenna switching technique.
Nanoparticles target distinct dendritic cell populations according to their size (2008)

The efficiency of a vaccine largely depends on the appropriate targeting of the innate immune system, mainly through prolonged delivery of antigens and immunomodulatory substances to professional antigen-presenting cells in the lymphoid environment. Particulate antigens, such as virus-like particles (VLP) induce potent immune responses. However, little is known about the relative importance of direct drainage of free antigen to lymph nodes (LN) versus cellular transport and the impact of particle size on the process. Here, we show that nanoparticles traffic to the draining LN in a size-dependent manner. Whereas large particles (500-2000 nm) were mostly associated with dendritic cells (DC) from the injection site, small (20-200 nm) nanoparticles and VLP (30 nm) were also found in LN-resident DC and macrophages, suggesting free drainage of these particles to the LN. In vivo imaging studies in mice conditionally depleted of DC confirmed the capacity of small but not large particles to drain freely to the LN and demonstrated that DC are strictly required for transport of large particles from the injection site to the LN. These data provide evidence that particle size determines the mechanism of trafficking to the LN and show that only small nanoparticles can specifically target LN-resident cells.
A Case Evaluation in Internet Assisted Laboratory Teaching (2001)

This paper presents the preliminary findings from an evaluation conducted on the implementation of Internet assisted teaching of fluid mechanical engineering laboratory sessions on a university degree programme. This paper compares the particular merits of laboratory sessions in which students receive instruction from Internet based presentations compared with those who received instruction from ‘traditional’ tutor led sessions, through a process of questionnaire based surveys and direct observations. The paper concludes that Internet assisted sessions allow students to easily repeat experimental instructions and provide late or absent students the opportunity to easily catch up with missed work. However, students still appear more likely to ask staff for assistance than rely on the Internet resources for information, and the Internet based programme, as applied here, has encountered some practical difficulties that have greatly reduced its effectiveness as a teaching aid.
Controlled release of poly-D,L-lactic acid containing bleomycin (1995)

By use of four types of in vivo degradable polylactic acid (PLA), i.e. PLA with an average molecular weight of 1500 (1500DL), 2200 (2200DL), 2800 (2800DL) and 3500 (3500DL), preparations of bleomycin (BLM)-containing solid forms (polymers) were tested. The in vitro release of BLM from the polymers was also examined in an immersion system. By the melt-pressing technique, five types of BLM (2.5 mg) containing solid forms, i.e. 1500DL polymer, 2200DL polymer, 2800DL polymer, 3500DL polymer and 1500DL + 3500DL (a mixture of 1500DL and 3500DL) polymer were prepared. In all five types of polymers, cumulative BLM release was controlled to less than 5% by the third day and no initial burst of the release was observed. BLM release from the polymer continued for 3 weeks at the shortest and 6 weeks at the longest. Various polymers containing BLM could be useful for the site of drug administration or anti-cancer release pattern.
Boosted Regression Active Shape Models (2007)

We present an efficient method of fitting a set of local feature models to an image within the popular Active Shape Model (ASM) framework [3]. We compare two different types of non-linear boosted feature models trained using GentleBoost [9]. The first type is a conventional feature detector classifier, which learns a discrimination function between the appearance of a feature and the local neighbourhood. The second local model type is a boosted regression predictor which learns the relationship between the local neighbourhood appearance and the displacement from the true feature location. At run-time the second regression model is much more efficient as only the current feature patch needs to be processed. We show that within the local iterative search of the ASM the local feature regression provides improved localisation on two publicly available human face test sets as well as increasing the search speed by a factor of eight.
Taneční drogy a akutní kardiologie (2006)

V tomto cisle je publikovana kazuistika dr. Holaje a spolupracovniků, popisujici připad mladeho muže, který po užiti drogy metylen 3,4dioxyamfetaminu (MDMA), spise zname pod nazvem extaze, prodělal akutni infarkt myokardu. Upozorňuji tak na specifický problem, který je již dobře znamý v zapadni Evropě a severni Americe. Je jim akutni srdecni onemocněni vznikle v důsledku uživani takzvaných rekreacnich drog. Nabizi se otazka, zda popisovaný připad bude zcela ojedinělý nebo zda je předzvěsti toho, s cim se v klinicke praxi budeme v nasledujicich letech stale castěji setkavat.
SUN-006 Lasofoxifene Achieves Potent Anti-Tumor Activity in Hormone-Resistant Breast Tumors by Maintaining High Affinity Binding for Y537S ERα (2019)

Activating somatic mutations to the ligand binding domain (LBD) of ESR1 (the gene for estrogen receptor alpha) may arise after prolonged hormone treatments. Y537S and D538G are the most prevalent mutations, accounting for 16% and 32% of cases respectively. Both mutations resist tamoxifen by favoring the receptor’s active state in the absence of hormone to reduce the selective estrogen receptor modulator’s binding affinity. Fulvestrant, a selective estrogen receptor degrader, is the only clinically approved molecule that can fully ablate Y537S estrogen receptor alpha (ERα) transcriptional activity, but does so at decreased potency due to a reduced ligand binding affinity. Interestingly, lasofoxifene, a selective estrogen receptor modulator, possesses significant anti-tumor activity in preclinical models of luminal breast cancers harboring WT, Y537S, and D538G ERα. Comprehensive structural and biochemical studies were undertaken to determine the molecular basis for lasofoxifene potency towards Y537S ERα. X-ray crystal structures of lasofoxifene in complex with WT and Y537S ERα LBD show that the molecule adopts an identical conformation within the hormone binding pocket. While, a competitive ligand binding assay shows that lasofoxifene possesses the same affinity towards WT and Y537S ERα LBD in vitro, compared to 5-10 fold decrease compared to other SERMs and SERDs. Collectively, these data suggest that lasofoxifene achieves improved anti-tumor activity by retaining its high affinity binding for the Y537S ERα somatic mutant.
Ultrafast Laser Radiation and Conduction Heat Transfer in Biological Tissues (2005)

Ultrafast laser radiation heat transfer in biological tissues is governed by time-dependent equation of radiative transfer and modeled using the transient discrete ordinates method. The divergence of radiative heat flux is then obtained and used for predicting the local temperature response due to radiation energy absorption within the ultrashort time period. To this end, the lumped method is employed and heat diffusion is negligible. Both single pulse and pulse train irradiations are considered. For the single pulse irradiation, the transient radiation field is obtained and the local temperature keeps rising until a time of about 20 times of the short pulse width; and then a stable local temperature profile is reached and maintained until the start of heat conduction. For the pulse train case (104 ultrashort pulses until 1 ms), the local temperature response is an accumulation of continuous single pulses because the thermal relaxation time of biological tissues was reported in the range of 1-100 sec and is much longer than the pulse train duration (1 ms). After a stable local temperature field is achieved, the hyperbolic heat conduction model is adopted to describe the heat conduction. MacCormark’s scheme is utilized for solving the thermal wave equations. Thermal wave behavior is observed during the heat transfer process. It is found that the hyperbolic wave model predicts a higher temperature rise than the classical heat diffusion model. After several thermal relaxation times the thermal wave behavior is substantially weakened and the predictions between the hyperbolic and diffusion models match.
Synthetic Cyclic Oligosaccharides (1998)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic Cyclic OligosaccharidesGiuseppe Gattuso, Sergey A. Nepogodiev, and J. Fraser StoddartView Author Information School of Chemistry, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK Cite this: Chem. Rev. 1998, 98, 5, 1919–1958Publication Date (Web):June 25, 1998Publication History Received20 May 1997Revised8 April 1998Published online25 June 1998Published inissue 30 July 1998https://doi.org/10.1021/cr960133wCopyright © 1998 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views2487Altmetric-Citations102LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (1 MB) Get e-AlertsSUBJECTS:Anions,Free radicals,Oligosaccharides,Precursors,Reaction products Get e-Alerts
Angiogenic role of endogenous basic fibroblast growth factor released by rat aorta after injury. (1993)

The autocrine role of basic fibroblast growth factor (bFGF) in angiogenesis was studied in the rat aortic ring-collagen gel model using serum-free culture conditions. Immunohistochemical staining of the rat aorta showed bFGF in the cytoplasm of endothelial and smooth muscle cells. Aortic rings mechanically injured during the dissection procedure released bFGF, which was demonstrated in the conditioned medium by slot and Western blot analysis. bFGF-containing aorta-conditioned medium and purified bFGF increased both the number and length of microvessels sprouting from the explants. This effect was particularly evident during the second week of culture, when the release of endogenous bFGF was minimal. Neutralizing anti-bFGF antibodies induced a 40% reduction of angiogenesis. Regression of microvessels, which regularly occurred toward the end of the second week, was prevented by purified bFGF. These data support the idea that bFGF released by vascular cells plays an important role in the autoregulation of angiogenesis after injury.
Genetic Mapping of the minB Locus in Escherichia coli K-12 (1983)

The minB (minicell production) locus of Escherichia coli K-12 was mapped by transduction using bacteriophage P1. minB is located at min 25.6, between purB (min 25.2) and dadR (min 25.8). The mapping was facilitated by the use of insertion zcf-236::Tn10, which is inserted at min 25.4.
Microtubules and desmin filaments during onset of heart hypertrophy in rat: a double immunoelectron microscope study. (1987)

The distribution of tubulin and desmin, the constituent proteins of microtubules and intermediate filaments, respectively, were studied in normal and hypertrophied rat myocardium by high-resolution immunofluorescence and immunoelectron microscopy. Cardiac hypertrophy was induced in 25-day-old rats by aortic stenosis. In the normal heart, double immunolabelling of ultrathin frozen sections of papillary muscle using gold-labelled probes for tubulin and desmin showed that microtubules ran primarily in a longitudinal direction through the intermyofibrillar spaces, perpendicularly to the desmin filaments. Microtubules were present near nuclei, mitochondria, and plasma membranes, while desmin filaments formed transverse connections between adjacent Z disks. No tubulin was observed near the intercalated disks, which were rich in desmin filaments. In hypertrophied hearts, myocytes exhibited the typical morphological features of developing hypertrophy. While there was little difference in the distribution of the microtubules around mitochondria and at the plasma membrane, considerable increases were seen near the nuclei and along the myofibrils. Desmin labelling was distributed transversely as in the controls; however, sometimes it was longitudinally oriented either in the intermyofibrillar space linking 2 Z disks out of register or along digitations of the intercalated disks connecting neighboring desmosomes. The unique rearrangement of desmin and tubulin filaments in hypertrophied cardiac myocytes emphasizes their distinct role in myocyte organization. We suggest that, during the development of cardiac hypertrophy, desmin filaments are mainly involved in maintaining the myofibrils in register, whereas the degree of assembly of microtubules is correlated with the rate of protein synthesis and with myofibrillogenesis.
Approaching Capacity at High-Rates with Iterative Hard-Decision Decoding (2012)

A variety of low-density parity-check (LDPC) ensembles have now been observed to approach capacity with message-passing decoding. However, all of them use soft (i.e., non-binary) messages and a posteriori probability (APP) decoding of their component codes. In this paper, we show that one can approach capacity at high rates using iterative hard-decision decoding (HDD) of generalized product codes. Specifically, a class of spatially-coupled GLDPC codes with BCH component codes is considered, and it is observed that, in the high-rate regime, they can approach capacity under the proposed iterative HDD. These codes can be seen as generalized product codes and are closely related to braided block codes. An iterative HDD algorithm is proposed that enables one to analyze the performance of these codes via density evolution (DE).
CD30 Overexpression Enhances Negative Selection in the Thymus and Mediates Programmed Cell Death Via a Bcl-2-Sensitive Pathway (1999)

Abstract The biological function of CD30 in the thymus has been only partially elucidated, although recent data indicate that it may be involved in negative selection. Because CD30 is expressed only by a small subpopulation of medullary thymocytes, we generated transgenic (Tg) mice overexpressing CD30 in T lymphocytes to further address its role in T cell development. CD30 Tg mice have normal thymic size with a normal number and subset distribution of thymocytes. In vitro, in the absence of CD30 ligation, thymocytes of CD30 Tg mice have normal survival and responses to apoptotic stimuli such as radiation, dexamethasone, and Fas. However, in contrast to controls, CD30 Tg thymocytes are induced to undergo programmed cell death (PCD) upon cross-linking of CD30, and the simultaneous engagement of TCR and CD30 results in a synergistic increase in thymic PCD. CD30-mediated PCD requires caspase 1 and caspase 3, is not associated with the activation of NF-κB or c-Jun, but is totally prevented by Bcl-2. Furthermore, CD30 overexpression enhances the deletion of CD4+/CD8+ thymocytes induced by staphylococcal enterotoxin B superantigen and specific peptide. These findings suggest that CD30 may act as a costimulatory molecule in thymic negative selection.
Collagen in two muscles of sheep selected for weight as yearlings (1993)

Abstract Collagen concentration and solubility were examined in the semimembranosus and biceps femoris muscles of Romney lambs from the Hight line, a line selected for nine generations on the basis of high body weight at 1 year old. Lambs from the Control (CON) and high body weight (HBW) lines were slaughtered at five ages (0, 42, 70, 274, 365 days). In the semimembranosus muscle, there was no evidence of concentration or solubility differences between the lines at any age, but there was a subtle sex by age interaction, possibly because of increased collagen synthesis in males at puberty. In the biceps femoris muscle, collagen concentration was 14% higher in the HBW line, but there was no difference in solubility. The semimembranosus muscle was shear‐tested after cooking to give an objective measure of tenderness. Selection line did not affect shear force. With the semimembranosus muscle, collagen solubility was a better predictor of shear force than collagen concentration, confirming previous work on this muscle. Although the biceps femoris muscle was not shear‐tested, it is argued that the higher collagen concentration in the HBW line would cause only a minimal decrease in tenderness. The concentration and the solubility of collagen in the semimembranosus muscle were significant predictors of the same characteristics in the biceps femoris muscle, a finding that could be useful in a breeding programme.