gebhart/patrepeval huggingface dataset:
Distinct Role of CD80 and CD86 in the Regulation of the Activation of B Cell and B Cell Lymphoma (2002)

To date, not much has been known regarding the role of CD80 and CD86 molecules in signaling of B cells. The CD28/CTLA4 ligands, CD80 (B7-1) and CD86 (B7-2), are expressed on the surface of freshly isolated splenic B cells, and their expression is up-regulated by lipopolysaccharides. In the present study, we have investigated whether signaling via CD80/CD86 could alter the proliferation and immunoglobulin synthesis of B cells. Splenic B cells were stimulated with lipopolysaccharides in the presence of anti-B7-1 (16-10A1) and anti-B7-2 (GL1) monoclonal antibodies (mAbs). Exciting features observed during the study were that cross-linking of CD86 with GL1 enhanced the proliferation and production of IgG1 and IgG2a isotypes. In contrast, anti-B7-1 (16-10A1) mAb could efficiently block the proliferation and production of IgG1 and IgG2a. Furthermore, GL1 mAb could also induce the secretion of IgG isotypes from B cell lymphomas. Importantly, 16-10A1 could retard the growth of lymphomas and favored the up-regulation of pro-apoptotic molecules caspase-3, caspase-8, Fas, FasL, Bak, and Bax and down-regulation of anti-apoptotic molecule Bcl-x(L). In contrast, GL1 augmented the level of anti-apoptotic molecules Bcl-w and Bcl-x(L) and decreased the levels of pro-apoptotic molecule caspase-8, thereby providing a novel insight into the mechanism whereby triggering through CD80 and CD86 could deliver regulatory signals. Thus, this study is the first demonstration of a distinct signaling event induced by CD80 and CD86 molecules in B cell lymphoma. Finally, the significance of the finding is that CD80 provided negative signal for the proliferation and IgG secretion of normal B cells and B cell lymphomas. In contrast, CD86 encouraged the activity of B cells.
Flexible update propagation for weakly consistent replication (1997)

article Free Access Share on Flexible update propagation for weakly consistent replication Authors: Karin Petersen Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, California Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, CaliforniaView Profile , Mike J. Spreitzer Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, California Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, CaliforniaView Profile , Douglas B. Terry Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, California Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, CaliforniaView Profile , Marvin M. Theimer Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, California Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, CaliforniaView Profile , Alan J. Demers Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, California and Oracle Corporation, 500 Oracle Way, Redwood Shores, CA Computer Science Laboratory, Xerox Palo Alto Research Center, Palo Alto, California and Oracle Corporation, 500 Oracle Way, Redwood Shores, CAView Profile Authors Info & Claims ACM SIGOPS Operating Systems ReviewVolume 31Issue 5Dec. 1997 pp 288–301https://doi.org/10.1145/269005.266711Published:01 October 1997Publication History 338citation2,547DownloadsMetricsTotal Citations338Total Downloads2,547Last 12 Months109Last 6 weeks7 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Assessment of mRNA and microRNA Stabilization in Peripheral Human Blood for Multicenter Studies and Biobanks (2010)

In this study we evaluate the suitability of two methods of RNA conservation in blood samples, PAXgene and RNAlater, in combination with variable shipping conditions for their application in multicenter studies and biobanking. RNA yield, integrity, and purity as well as levels of selected mRNA and microRNA species were analyzed in peripheral human blood samples stabilized by PAXgene or RNAlater and shipped on dry ice or at ambient temperatures from the study centers to the central analysis laboratory. Both examined systems were clearly appropriate for RNA stabilization in human blood independently of the shipping conditions. The isolated RNA is characterized by good quantity and quality and well suited for downstream applications like quantitative RT-PCR analysis of mRNA and microRNA. Superior yield and integrity values were received using RNAlater. It would be reasonable to consider the production and approval of blood collection tubes prefilled with RNAlater to facilitate the use of this excellent RNA stabilization system in large studies.
Selective formation of large-grained, (100)- or (111)-oriented Si on glass by Al-induced layer exchange (2014)

By controlling the Si thickness and the annealing temperature used for Al-induced crystallization, we controlled the fraction of (100) and (111) orientations of polycrystalline Si (poly-Si) grains grown on glass. Changing the proportions of crystal orientation strongly influenced the average grain size of the poly-Si layer. By growing a 99% (111)-oriented poly-Si layer, formed with a 50-nm-thick Si layer at 375 °C, we produced a Si layer with grains nearly 40 μm in size. We discuss the growth mechanism from the perspective of competition between (100)- and (111)-oriented nuclei. This achievement holds promise for fabricating high-efficiency thin-film solar cells on inexpensive glass substrates.
Movie-DiC: a Movie Dialogue Corpus for Research and Development (2012)

This paper describes Movie-DiC a Movie Dialogue Corpus recently collected for research and development purposes. The collected dataset comprises 132,229 dialogues containing a total of 764,146 turns that have been extracted from 753 movies. Details on how the data collection has been created and how it is structured are provided along with its main statistics and characteristics. 1
Instruction fetch energy reduction using loop caches for embedded applications with small tight loops (1999)

A fair amount of work has been done in recent years on reducing power consumption in caches by using a small instruction buffer placed between the execution pipe and a larger main cache [ 1,2, These techniques, however, often degrade the overall system performance. In this paper, we propose using a small instruction buffer, also called a loop cache, to save power. A loop cache has no address tag store. It consists of a direct-mapped data array and a loop cache controller. The loop cache controller knows precisely whether the next instruction request will hit in the loop cache, well ahead of time. As a result, there is no performance degradation.
A photobiological and photophysical-based study of phototoxicity of two chlorins. (2001)

To understand the fundamental determinants of phototoxic efficacy and absorbed photodynamic dose, the triplet state and photobleaching quantum yields in living cells, cellular uptake, intracellular localization, and correlation with cell viability were studied for the two purpurins tin ethyl etiopurpurin 1 (SnET2) and tin octaethylbenzochlorin (SnOEBC) in ovarian cancer cells (OVCAR5). Although the triplet yields of these two photosensitizers were not significantly affected by cellular incorporation, the photobleaching yields were shown to be 3 orders of magnitude higher for cellular-bound sensitizer than for free or albumin-bound photosensitizer and higher for SnET2 than for SnOEBC for all of the cases. The intracellular concentration of SnOEBC was half that of SnET2 after 3 h- and 24 h-incubation times for both 0.1 microM and 1.0 microM incubation concentrations. Despite the lower concentrations of SnOEBC, the phototoxicity of the two photosensitizers was comparable at 1-microM incubation concentration and was up to 10-fold higher for SnOEBC at the lower concentration. The subcellular localization established using confocal microscopy and molecular probes showed that both photosensitizers were primarily lysosomally localized. SnOEBC, however, had an extra-lysosomal, mitochondrial localization component. The photophysical measurements allowed calculation of the intracellular singlet oxygen production, which indicated that the photosensitizer-light dose reciprocity was limited by photobleaching for SnET2 but only minimally for SnOEBC, and this was confirmed through cell-survival studies. Taken together, these data indicate that the critical determinant of differences in phototoxicity between the two molecules was their relative rates of photobleaching and their subcellular localization. The study points to the importance of combining photosensitizer uptake and localization with photophysical measurements in the relevant biological milieu to reasonably interpret and/or predict photosensitization efficacies.
Potent Inactivation of Cathepsins S and L by Peptidyl (Acyloxy)methyl Ketones (1994)

Peptidyl (acyloxy)methyl ketones (Z-Aa-Aa-CH2-O-CO-R), a new class of irreversible inhibitors whose chemical reactivity can be modulated by varying the substitution pattern of the carboxylate leaving group, are shown to be extremely potent inactivators of the lysosomal cysteine proteinases cathepsin L and cathepsin S. The highest k2/Ki values measured were found to exceed 10(6) M-1s-1 for both cathepsin L and cathepsin S. The rate of inactivation can be controlled by varying the dipeptidyl moiety or the carboxylate leaving group, with the second-order rate constants for both enzymes found to be strongly dependent on the pKa values of the leaving group. The specificities of the cathepsins S and L reveal a different selectivity towards the nature of substitution of the aryl P' leaving group of the inhibitor. This new inhibitor class opens the possibility of the design of selective and specific inhibitors for lysosomal cysteine proteinases.
Promoter prediction in the human genome (2001)

Computational prediction of eukaryotic poIII promoters has been one of the most elusive problems despite considerable effort devoted to the study. Researchers have looked for various types of signals around the transcriptional start site (TSS), viz. oligo-nucleotide statistics, potential binding sites for core factors, clusters of binding sites, proximity to CpG islands etc. The proximity of CpG islands to gene starts is now a well established fact, although until recently, it was based on very little genomic data. In this work we explore the possibility of enhancing the promoter prediction accuracy by combining CpG island information with a few other, biologically motivated, seemingly independent signals, that cover most of the known knowledge. We benchmarked the method on a much larger genomic datasets compared to previous studies. We were able to improve slightly upon current prediction accuracy. Furthermore, we observe that CpG islands are the most dominant signals and the other signals do not improve the prediction. This suggests that the computational prediction of promoters for genes with no associated CpG-island (typically having tissue-specific expression) looking only at the immediate neighborhood of the TSS may not even be possible. We suggest some biological experiments and studies to better understand the biology of transcription.
The golden code: a 2 x 2 full-rate space-time code with non-vanishing determinants (2004)

In this paper we present the Golden code for a 2times2 MIMO system. This is a full-rate 2times2 linear dispersion algebraic space-time code with unprecedented performance based on the Golden number 1+radic5/2
Selected Methods for Antibody and Nucleic Acid Probes (1993)

A basic manual for individuals beginning to use antibody or nucleic acid probes, providing a sufficient introduction to each technique so that readers may then make use of methods published in journals and other primary sources. Special emphasis is placed on techniques of particular importance to ne
Delta-doping of Semiconductors (2005)

Part I: 1. Introduction E. F. Schubert Part II: 2. Electronic structure of delta-doped semiconductors C. R. Proetto Part III: 3. Recent progress in delta-like confinement of impurities in GaAs K. H. Ploog 4. Flow-rate modulation epitaxy (FME) of III-V semiconductors T. Makimoto and Y. Horikoshi 5. Gas source molecular beam epitaxy (MBE) of delta-doped III-V semiconductors D. Ritter 6. Solid phase epitaxy for delta-doping in silicon I. Eisele 7. Low temperature MBE of silicon H.-J. Gossmann Part IV: 8. Secondary ion mass spectrometry of delta-doped semiconductors H. S. Luftmann 9. Capacitance-voltage profiling E. F. Schubert 10. Redistribution of impurities in III-V semiconductors E. F. Schubert 11. Dopant diffusion and segregation in delta-doped silicon films H.-J. Gossmann 12. Characterisation of silicon and delta-doped structures in GaAs R. C. Newman 13. The DX-center in silicon delta-doped GaAs and AlxGa1-xAs P. M. Koenraad Part V: 14. Luminescence and ellipsometry spectroscopy H. Yao and E. F. Schubert 15. Photoluminescence and Raman spectroscopy of single delta-doped III-V semiconductor heterostructures J. Wagner and D. Richards 16. Electron transport in delta-doped quantum wells W. T. Masselink 17. Electron mobility in delta-doped layers P. M. Koenraad 18. Hot electrons in delta-doped GaAs M. Asche 19. Ordered delta-doping R. L. Headrick, L. C. Feldman and B. E. Weir Part IV: 20. Delta-doped channel III-V field effect transistors (FETs) W.-P. Hong 21. Selectively doped heterostructure devices E. F. Schubert 22. Silicon atomic layer doping FET K. Nakagawa and K. Yamaguchi 23. Planar doped barrier devices R. J. Malik 24. Silicon interband and intersubband photodetectors I. Eisele 25. Doping superlattice devices E. F. Schubert.
A novel cationic cardiolipin analogue for gene delivery. (2006)

The optically active R and S isomers of cationic cardiolipin analogues (CCA) were synthesized and evaluated as a liposome based transfection reagent. Both isomers form stable liposomes with mean diameters of about 120 nm without any additional lipid ingredients. No significant change in particle size distribution profile was observed over one-month storage at room temperature (20-25 degrees C). The gel to liquid crystalline phase transition temperature (Tm) of cationic liposomes comprised of both R and S isomers was approximately 2 degrees C, as measured by differential scanning calorimetry (DSC). Both isomers also formed stable liposomes when combined with DOPE. In vitro transfection efficiency of the CCA/DOPE liposomes complexed to plasmid DNA was evaluated using a luciferase reporter gene. Both liposomes composed of R and S isomers of the cationic cardiolipin displayed higher transfection efficiency than commercially available Lipofectin. Further in vivo studies are warranted.
Small hairpin RNA as a small molecule sensor (2008)

ISSN:1744-4292
Screening Molecules for Control of Citrus Huanglongbing Using an Optimized Regeneration System for ‘Candidatus Liberibacter asiaticus’-Infected Periwinkle (Catharanthus roseus) Cuttings (2010)

Citrus huanglongbing is one of the most destructive diseases of citrus worldwide. The disease is associated with three different species of 'Candidatus Liberibacter', of which 'Ca. L. asiaticus' is the most widely distributed. An optimized system using 'Ca. L. asiaticus'-infected periwinkle cuttings was developed to screen chemical compounds effective for controlling the bacterial population while simultaneously assessing their phytotoxicity. The optimal regeneration conditions were determined to be the use of vermiculite as a growth medium for the cuttings, and a fertilization routine using half-strength Murashige and Tucker medium supplemented with both naphthalene acetic acid (4 microg/ml) and indole-3-butyric acid (4 microg/ml). This system allowed a plant regeneration rate of 60.6% for 'Ca. L. asiaticus'-infected cuttings in contrast to the <1% regeneration rate with water alone. Two chemical agents, penicillin G sodium and 2,2-dibromo-3-nitrilopropionamide (DBNPA), were found to be effective at eliminating or suppressing the 'Ca. L. asiaticus' bacterium in this periwinkle regeneration system. When treated with penicillin G sodium at 50 microg/ml, all plants regenerated from 'Ca. L. asiaticus'-infected cuttings were 'Ca. L. asiaticus' negative as determined by both nested polymerase chain reaction (PCR) and quantitative real-time PCR. In addition, DBNPA was also able to significantly reduce the percentage of 'Ca. L. asiaticus'-positive plants and the titer of the 'Ca. L. asiaticus' bacterium at 200 microl/liter.
Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248 through 2312, which overlap a phospholipid-binding site (1995)

The finding that human factor VIII (fVIII) inhibitor antibodies with C2 domain epitopes interfere with the binding of fVIII to phosphatidylserine (PS) suggested that this is the mechanism by which they inactivate fVIII. We constructed a recombinant C2 domain polypeptide and demonstrated that it bound to all six human inhibitors with fVIII light chain specificity. Thus, some antibodies within the polyclonal anti-light chain population require only amino acids within C2 for binding. Recombinant C2 also partially or completely neutralized the inhibitor titer of these plasmas, demonstrating that anti-C2 antibodies inhibit fVIII activity. Immunoblotting of a series of C2 deletion polypeptides, expressed in Escherichia coli, with inhibitor plasmas showed that the epitopes for human inhibitors consist of a common core of amino acid residues 2248 through 2312 with differing extensions for individual inhibitors. The epitope of inhibitory monoclonal antibody (MoAb) ESH8 was localized to residues 2248 through 2285. Three human antibodies and anti-C2 MoAb NMC-VIII/5 bound to a synthetic peptide consisting of amino acids 2303 through 2332, a PS-binding site, but MoAb ESH8 did not. These antibodies also inhibited the binding of fVIII to synthetic phospholipid membranes of PS and phosphatidylcholine, confirming that the blocked epitopes contribute to membrane binding as well as binding to PS. In contrast, MoAb ESH8 did not inhibit binding. As the maximal function of activated fVIII in the intrinsic factor Xase complex requires its binding to a phospholipid membrane, we propose that fVIII inhibition by anti-C2 antibodies is related to the overlap of their epitopes with the PS-binding site. MoAb ESH8 did not inhibit fVIII binding to PS-containing membranes, suggesting the existence of a second mechanism of fVIII inhibition by anti-C2 antibodies.
CTA for Screening of Complicated Atherosclerotic Carotid Plaque—American Heart Association Type VI Lesions as Defined by MRI (2013)

Maximum soft plaque component thickness as measured by carotid CTA is a reliable indicator of a complicated plaque, with a threshold of 2.2 mm representing little to no probability of a complicated American Heart Association lesion type VI plaque.
Hierarchical system test by an IEEE 1149.5 MTM-bus slave-module interface core (2000)

An IEEE 1149.5 module test and maintenance (MTM) bus slave module interface core is presented, which is used for direct access from the system bus to the IEEE 1149.1 chip-level or on-chip buses to facilitate hierarchical system test and diagnosis. The hierarchical test methodology also is presented, which is applicable to the system-on-chip environment, All the standard 1149.1 instructions, such as SAMPLE/PRELOAD, EXTEST, BYPASS, and even RUNBIST, can be performed within three 1149.5 read/write-data message cycles. The messages are transmitted between the MTM-bus master module (Ill-module) and the slave module (S-module). We adopt the full test access port control method to activate the 1149.1 boundary-scan paths via the 1149.5 MTM-bus. Our S-module interface circuit implements 16 CORE commands and one read/write-data command. It has been prototyped using a field-programmable gate array chip and implemented by a full-custom chip. Hierarchical test of multiple 1149.1 compatible boards has been experimentally verified.
Transition metal borohydride complexes. II. Th reaction of copper(I) compounds with boron hydride anions (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTransition metal borohydride complexes. II. Th reaction of copper(I) compounds with boron hydride anionsStephen J. Lippard and David UckoCite this: Inorg. Chem. 1968, 7, 6, 1051–1056Publication Date (Print):June 1, 1968Publication History Published online1 May 2002Published inissue 1 June 1968https://pubs.acs.org/doi/10.1021/ic50064a002https://doi.org/10.1021/ic50064a002research-articleACS PublicationsRequest reuse permissionsArticle Views2321Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
MspI, an isoschizomer of HpaII which cleaves both unmethylated and methylated HpaII sites (1978)

The cleavage of DNA by restriction endonucleases HpaII and HapII is prevented by the presence of a 5-methyl group at the internal C residue of its recognition sequence CCGG. MspI, an isoschizomer of HpaII available from New England Biolabs, cleaves DNA irrespective of the presence of a methyl group at this position. This enzyme cleaves DNA from Haemophilus parainfluenzae and Haemophilus aphrophilus readily while HpaII and HapII cannot degrade these DNAs. Practically all HpaII sites in mammalian sperm DNA are also protected by methylation at the internal C position since HpaII and HapII barely cleave this DNA (average molecular weight 40 kb). MspI, however, cleaves the DNA to an average size of about 5 kb.
Cardiovascular disease and Alzheimer's disease: common links (2006)

Growing evidence supports a strong and likely causal association between cardiovascular disease (CVD), and its risk factors, with incidence of cognitive decline and Alzheimer's disease. Individuals with subclinical CVD are at higher risk for dementia and Alzheimer's. Several cardiovascular risk factors are also risk factors for dementia, including hypertension, high LDL cholesterol, low HDL cholesterol and especially diabetes. Moderate alcohol appears to be protective for both CVD and dementia. In contrast, inflammatory markers predict cardiovascular risk, but not dementia, despite biological plausibility for such a link. The substantial overlap in risk factors points to new avenues for research and prevention.