gebhart/patrepeval huggingface dataset:
Laminin promotes rabbit neutrophil motility and attachment. (1986)

Polymorphonuclear neutrophils (PMN) traverse basement membrane to reach sites of infection. We have studied the role of laminin, a specific basement membrane component, in this process using three assay systems. In the Boyden chamber, laminin was found to stimulate chemotaxis of neutrophils while fibronectin did not. Co-incubation of cells with antibody to laminin blocked this chemotaxis, while antibody to fibronectin was without effect. In the human amnion system, neutrophils were shown to penetrate through the tissue when the peptide chemoattractant f-Met-Leu-Phe was placed on the opposing side. Antibody to laminin, but not to fibronectin, blocked this penetration. In an attachment assay system, laminin, but not fibronectin, was found to increase dispase-treated neutrophil attachment to type IV (basement membrane) collagen-coated plastic and to a plastic substrate itself. Electrophoretic analysis of PMN extract indicated the presence of laminin, and indirect immunofluorescence suggested that laminin is localized on the surface of the neutrophils. These data suggest that PMN can bind laminin on their cell surfaces, use laminin to attach to basement (type IV) membrane collagen, and migrate toward a gradient of laminin. These properties may be important for the passage of neutrophils from the circulation to sites of infection.
Electroorganic chemistry. 118. Electroreductive intermolecular coupling of ketones with olefins (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectroorganic chemistry. 118. Electroreductive intermolecular coupling of ketones with olefinsTatsuya Shono, Shigenori Kashimura, Yasuo Mori, Takashi Hayashi, Takeshi Soejima, and Yoshihide YamaguchiCite this: J. Org. Chem. 1989, 54, 26, 6001–6003Publication Date (Print):December 1, 1989Publication History Published online1 May 2002Published inissue 1 December 1989https://pubs.acs.org/doi/10.1021/jo00287a001https://doi.org/10.1021/jo00287a001research-articleACS PublicationsRequest reuse permissionsArticle Views851Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Forward and Reverse Genetic Approaches to Behavior in the Mouse (1994)

Modern molecular genetic and genomic approaches are revolutionizing the study of behavior in the mouse. "Reverse genetics" (from gene to phenotype) with targeted gene transfer provides a powerful tool to dissect behavior and has been used successfully to study the effects of null mutations in genes implicated in the regulation of long-term potentiation and spatial learning in mice. In addition, "forward genetics" (from phenotype to gene) with high-efficiency mutagenesis in the mouse can uncover unknown genes and has been used to isolate a behavioral mutant of the circadian system. With the recent availability of high-density genetic maps and physical mapping resources, positional cloning of virtually any mutation is now feasible in the mouse. Together, these approaches permit a molecular analysis of both known and previously unknown genes regulating behavior.
Hydroxyectoine Is Superior to Trehalose for Anhydrobiotic Engineering of Pseudomonas putida KT2440 (2002)

Anhydrobiotic engineering aims to increase the level of desiccation tolerance in sensitive organisms to that observed in true anhydrobiotes. In addition to a suitable extracellular drying excipient, a key factor for anhydrobiotic engineering of gram-negative enterobacteria seems to be the generation of high intracellular concentrations of the nonreducing disaccharide trehalose, which can be achieved by osmotic induction. In the soil bacterium Pseudomonas putida KT2440, however, only limited amounts of trehalose are naturally accumulated in defined high-osmolarity medium, correlating with relatively poor survival of desiccated cultures. Based on the enterobacterial model, it was proposed that increasing intracellular trehalose concentration in P. putida KT2440 should improve survival. Using genetic engineering techniques, intracellular trehalose concentrations were obtained which were similar to or greater than those in enterobacteria, but this did not translate into improved desiccation tolerance. Therefore, at least for some populations of microorganisms, trehalose does not appear to provide full protection against desiccation damage, even when present at high concentrations both inside and outside the cell. For P. putida KT2440, it was shown that this was not due to a natural limit in desiccation tolerance since successful anhydrobiotic engineering was achieved by use of a different drying excipient, hydroxyectoine, with osmotically preconditioned bacteria for which 40 to 60% viability was maintained over extended periods (up to 42 days) in the dry state. Hydroxyectoine therefore has considerable potential for the improvement of desiccation tolerance in sensitive microorganisms, particularly for those recalcitrant to trehalose.
Microcomputed tomography colonography for polyp detection in an in vivo mouse tumor model (2005)

This study was initiated to evaluate the efficacy of negative contrast-enhanced microcomputed tomography (microCT) colonography for the noninvasive detection of colonic tumors in living mice. After colonic preparation, 20 anesthetized congenic mice were scanned with high-resolution microCT. Images were displayed by using commercial visualization software and interpreted by two gastrointestinal radiologists, who were unaware of tumor prevalence and findings at gross pathology. Two-dimensional multiplanar images were assessed by using a five-point scale to distinguish colonic tumors (polyps) from fecal pellets (5 = definitely a tumor, 4 = probably a tumor, 3 = indeterminate, 2 = probably not a tumor, 1 = definitely not a tumor). Gross pathologic evaluation of excised mouse colons served as the reference standard. Data analysis included dichotomizing results, with 1-2 indicating no tumor and 3-5 indicating tumor and also receiver operator characteristic curve analysis with area under the curve for threshold-independent assessment. A total of 41 colonic polyps in 18 of the 20 mice were identified at gross examination on necropsy, of which 30 measured 2-5 mm and 11 measured <2 mm in size. The pooled per-polyp sensitivity for lesions >2 mm was 93.3% (56/60). The pooled per-mouse sensitivity for polyps >2 mm was 97.1% (33/34). Pooled specificity for distinguishing fecal pellets from tumor was 98.5% (65/66). The combined area under the curve from receiver operator characteristic curve analysis was 0.810 +/- 0.038 (95% confidence interval, 0.730-0.890). These findings indicate that accurate noninvasive longitudinal monitoring of colon tumor progression or response to various therapies is now technically feasible in live mice by using this microCT colonography method.
Decision Analysis (1987)

EXCELLENT clinical judgment requires optimal decision making. Many of the decisions that physicians make in their practices involve little uncertainty and little risk: these rote or routine choices need no special contemplation because they are "tried and true" practices. But for each routine problem there are several for which no easy solution is at hand. To deal with these, the tough problems, a physician can search for a properly designed, double-blind controlled study that examined patients of the same age, sex, and race and with the same conditions in the same stage; use an algorithm developed for such patients; use . . .
NaCl Induces a Na+/H+ Antiport in Tonoplast Vesicles from Barley Roots (1988)

Evidence was found for a Na(+)/H(+) antiport in tonoplast vesicles isolated from barley (Hordeum vulgare L. cv California Mariout 72) roots. The activity of the antiport was observed only in membranes from roots that were grown in NaCl. Measurements of acridine orange fluorescence were used to estimate relative proton influx and efflux from the vesicles. Addition of MgATP to vesicles from a tonoplast-enriched fraction caused the formation of a pH gradient, interior acid, across the vesicle membranes. EDTA was added to inhibit the ATPase, by chelating Mg(2+), and the pH gradient gradually dissipated. When 50 millimolar K(+) or Na(+) was added along with the EDTA to vesicles from control roots, the salts caused a slight increase in the rate of dissipation of the pH gradient, as did the addition of 50 millimolar K(+) to vesicles from salt-grown roots. However, when 50 millimolar Na(+) was added to vesicles from salt-grown roots it caused a 7-fold increase in the proton efflux. Inclusion of 20 millimolar K(+) and 1 micromolar valinomycin in the assay buffer did not affect this rapid Na(+)/H(+) exchange. The Na(+)/H(+) exchange rate for vesicles from salt-grown roots showed saturation kinetics with respect to Na(+) concentration, with an apparent K(m) for Na(+) of 9 millimolar. The rate of Na(+)/H(+) exchange with 10 millimolar Na(+) was inhibited 97% by 0.1 millimolar dodecyltriethylammonium.
Treatment of Ethylene Glycol Poisoning With Ethyl Alcohol (1965)

In the course of phylogenetic evolution, the substrate specificity of alcohol dehydrogenase, the enzyme which detoxicates ethyl alcohol, has apparently broadened. Thus, while the enzyme from yeast oxidizes ethyl alcohol and only a few other alcohols,<sup>1</sup>the enzyme of the horse liver is capable of oxidizing a range of other alcohols.<sup>1,2</sup>The human enzyme has an even wider range,<sup>3</sup>since contrary to the horse enzyme it oxidizes both ethyl alcohol and ethylene glycol. While chemically ethylene glycol is very similar to ethyl alcohol, both these compounds are toxic to man. Detailed studies of the enzymatic oxidation of ethylene glycol demonstrate that ethyl alcohol, the natural substrate, is a potent competitive inhibitor of the oxidation of ethylene glycol.<sup>3</sup>Hence, low concentrations of ethyl alcohol prevent the oxidation of large concentrations of ethylene glycol. These biochemical features of the catalytic behavior of human-liver alcohol dehydrogenase have served as
Mutagenic Polymerase Chain Reaction of Protein-Coding Genes for In Vitro Evolution (2002)

In vitro protein evolution is an efficient approach to study the structure and function of a protein, or to enhance its industrial utility (). One round of evolution consists of random mutation of a protein-coding gene, expression the resulting library in a population of micro-organisms, and high-throughput screening or selection of clones that most strongly exhibit a desired phenotype (“winners”). After many rounds, mutations that confer the phenotype accumulate on a single allele, e.g., the authors have isolated an octuple mutant of the Escherichia coli β-glucuronidase with catalytic activity resistant to roughly 80-fold higher concentrations of glutaraldehyde than that of the wild-type enzyme (). Here we describe a variation of the mutagenic polymerase chain reaction (PCR) (,) is described. The advantages of this method over other random mutagenesis techniques are explained.
Photo annotation on a camera phone (2004)

In this paper we describe a system that allows users to annotate digital photos at the time of capture. The system uses camera phones with a lightweight client application and a server to store the images and metadata and assists the user in annotation on the camera phone by providing guesses about the location and content of the photos. By conducting user interface testing, surveys, and focus groups we were able to evaluate the usability of this system and uncover usage patterns and motivations that will inform our development of future mobile media annotation applications. In this paper we present usability issues encountered in using a camera phone as an image annotation device immediately after image capture and users' responses to use of such a system.
Pharmacology of methylphenidate, amphetamine enantiomers and pemoline in attention-deficit hyperactivity disorder (1997)

Racemic methylphenidate remains the drug of choice for attention-deficit hyperactivity disorder (ADHD). Methylphenidate appears to produce psychostimulation by inhibiting the presynaptic uptake of impulse-released dopamine. The absolute bioavailability of methylphenidate in humans is quite low and variable: mean 23 per cent for the therapeutic (+)-isomer and 5 per cent for the (−)-isomer. The primary site of presystemic metabolism may be the gut and/or intestinal wall. Brain concentrations of methylphenidate average eight times that of blood. A Tmax of 1·5–2·5 h, a Cmax of 6–15 ng/ml and a T1/2 of 2–3·5 h are typical. The area under the plasma concentration–time curves for immediate-release versus sustained-release formulations are nearly identical, but the relative efficacy is unresolved. Dextroamphetamine has generally been found to compare favourably with methylphenidate in ADHD; it acts through release of newly synthesized dopamine. Levoamphetamine is present as a minor component in a combination product (Adderall®), but the rationale for inclusion of the levo isomer remains unclear. Pemoline appears to both release and block the uptake of dopamine. Though rarely exhibiting sympathomimetic side-effects, potential hepatotoxicity relegates pemoline to a second-line status. © 1997 John Wiley & Sons, Ltd.
Self-Assembled Smectic Phases in Rod-Coil Block Copolymers (1996)

Rod-coil block copolymers are self-assembling polymers that combine the physics of orientational ordering of rodlike polymers and the microphase separation of coil-coil block copolymers. Several new solid-state morphologies were observed in a series of anionically synthesized model poly(hexyl isocyanate-b-styrene) rod-coil diblock copolymers examined by transmission electron microscopy and selected-area electron diffraction. The rod-coils formed smectic C-like and O-like morphologies with domain sizes ranging from tens of nanometers to almost 1 micrometer. Both structural and orientational changes were found for increasing rod volume fractions. In addition, some morphologies exhibited spontaneous long-range orientational order over many tens of micrometers.
Enzyme abnormalities of patients with acquired immunodeficiency syndrome. (1988)

We determined the enzyme activities of glucose-6-phosphate isomerase, alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase in serum from 23 normal controls, 27 anti-HIV seropositive individuals confirmed by Western blot, and 53 patients with acquired immunodeficiency syndrome (AIDS). There is a significant difference for all four enzyme activities among controls, HIV seropositive individuals, and patients with AIDS, the enzyme activities showing a progressive increase as the disease progresses. Evidently these enzyme measurements may be adjunctive biochemical markers for progression of AIDS.
The Follicle-Stimulating Hormone Receptor: Biochemistry, Molecular Biology, Physiology, and Pathophysiology* (1997)

I. Introduction II. Biochemical Properties of the FSH Receptor: A Historical Prelude III. Molecular Structure of the FSH Receptor A. Cloning of the FSH receptor B. Predicted primary structure of the FSH receptor C. Molecular mass of the FSH receptor IV. The FSH Receptor Gene A. Chromosomal localization B. Structure and organization of the FSH receptor gene C. The promoter of the FSH receptor gene V. Expression of the FSH Receptor and Its Regulation A. FSH receptor gene expression B. Expression of the FSH receptor in the testis C. Expression of the FSH receptor in the ovary VI. Expression of the FSH Receptor in Cell Lines A. Cell lines expressing the recombinant FSH receptor B
Structural requirements for IgM assembly and cytolytic activity. Effects of mutations in the oligosaccharide acceptor site at Asn402. (1989)

Glycosylation of IgG occurs at asparagine 297 of the gamma H chain and is necessary for the normal capacity of IgG to activate the classical pathway of complement-dependent cytolysis. IgM is glycosylated at five sites in the constant region of the mu H chain, of which glycosylation at asparagine 402 seems analogous to the glycosylation of IgG. In order to assess the importance of glycosylation at asparagine 402 for IgM cytolytic activity, we have used site-directed mutagenesis to produce IgM which is not glycosylated at this position. In particular we have tested the effects of substituting Gln for Asn 402 and Thr-Gly for Gly 403-Thr 404 in the third constant region domain. We tested the effects of these substitutions by expressing the mutant mu genes in hybridoma cells which produce the hapten-specific kappa-chain. The normal mu-chain is glycosylated at Asn 402, and, as expected, these mutations appear to abrogate glycosylation of the mutant mu-chains at position 402 and do not affect the hapten affinity of the IgM. However, both of these mutations cause the increased production of monomeric rather than polymeric IgM: the ratio of monomeric to polymeric IgM is 0.21, 3.5, and 10.3 for wild-type IgM, IgM-Gln 402, and IgM-Thr 403-Gly 404, respectively. The wild-type and mutant polymeric IgM preparations were compared for their capacity to promote complement-dependent cytolysis: IgM-Gln 402 and IgM-Thr 403-Gly 404 have approximately 31% and 4%, respectively, of the capacity of wild-type IgM.
Semantic knowledge extraction and annotation for web images (2005)

Nowadays, images have become widely available on the World Wide Web (WWW). It's essential to develop effective ways for managing and retrieving such abundant images. Advantageously, compared to the traditional images where very little information is provided, the web images contain plentiful context data. This paper introduces a system that can automatically acquire semantic knowledge for web image annotation. By using a page layout analysis method that can precisely assign context to web images, we developed efficient algorithms to extract semantic knowledge for web images, such as description, people, temporal and geographic information. To validate the practicality and efficiency of this system, we applied it to about 6,500 images crawled from Web. Experiments demonstrated that our approach could achieve satisfactory results.
Wall-associated Kinase WAK1 Interacts with Cell Wall Pectins in a Calcium-induced Conformation (2005)

Wall-associated kinase 1 (WAK1) is a transmembrane protein containing a cytoplasmic Ser/Thr kinase domain and an extracellular domain in contact with the pectin fraction of the plant cell walls. In order to characterize further the interaction of WAK1 with pectin, a 564 bp DNA sequence corresponding to amino acids 67-254 of the extracellular domain of WAK1 from Arabidopsis thaliana was cloned and expressed as a soluble recombinant peptide in yeast. Using enzyme-linked immunosorbent assays (ELISA), we show that peptide WAK(67-254) binds to polygalacturonic acid (PGA), oligogalacturonides, pectins extracted from A. thaliana cell walls and to structurally related alginates. Our results suggest that both ionic and steric interactions are required to match the relatively linear pectin backbone. Binding of WAK(67-254) to PGA, oligogalacturonides and alginates occurred only in the presence of calcium and in ionic conditions promoting the formation of calcium bridges between oligo-and polymers (also known as 'egg-boxes'). The conditions inhibiting the formation of calcium bridges (EDTA treatment, calcium substitution, high NaCl concentrations, depolymerization and methylesterification of pectins) also inhibited the binding of WAK(67-254) to calcium-induced egg-boxes. The relevance of this non-covalent link between WAK(67-254) and cell wall pectins is discussed in terms of cell elongation, cell differentiation and host-pathogen interactions.
Endoplasmic reticulum targeting and glycosylation of hybrid proteins in transgenic tobacco. (1989)

The correct compartmentation of proteins to the endomembrane system, mitochondria, or chloroplasts requires an amino-terminal signal peptide. The major tuber protein of potato, patatin, has a signal peptide in common with many other plant storage proteins. When the putative signal peptide of patatin was fused to the bacterial reporter protein beta-glucuronidase, the fusion proteins were translocated to the endoplasmic reticulum in planta and in vitro. In addition, translocated beta-glucuronidase was modified by glycosylation, and the signal peptide was correctly processed. In the presence of an inhibitor of glycosylation, tunicamycin, the enzymatically active form of beta-glucuronidase was assembled in the endoplasmic reticulum. This is the first report of targeting a cytoplasmic protein to the endoplasmic reticulum of plants using a signal peptide.
Functional Intactness of Stimulatory and Inhibitory Autocrine Loops in Human Renal Carcinoma Cell Lines of the Clear Cell Type (1997)

Clear cell RCCs exhibit a complex and heterogeneous expression pattern for various growth factors and their receptors. Growth factor secretion and intact signal transduction pathways in most clear cell RCCs facilitate an intricate modulation of RCC growth by autocrine and paracrine interactions between tumor cells and host tissue.
Massive Rotating Stars: Pre--Supernova Evolution at Solar Metallicity (2005)

The concept of exploiting tumor intrinsic deficiencies in DNA damage repair mechanisms by inhibiting compensatory DNA repair pathways is well established. For example, ATM-deficient cells show increased sensitivity to the ATR inhibitor ceralasertib. DNA damage response (DDR)-deficient cells are also more sensitive to DNA damaging agents like the DNA crosslinker pyrrolobenzodiazepine (PBD) SG-3199. However, additional antitumor benefits from targeting the DDR pathways, which could operate through the activation of the innate immune system are less well studied. DNA accumulation in the cytosol acts as an immunogenic danger signal, inducing the expression of type-I interferon (IFN) stimulated genes (ISGs) by the activation of the cGAS-STING pathway. Here, we demonstrate that ATM <sup>-/-</sup> FaDu tumor cells have higher basal expression of ISGs when compared to WT cells and respond to ceralasertib and PBD SG-3199 by inducing higher levels of ISGs in a cGAS-STING-dependent manner. We show that sensitive tumor cells treated with ceralasertib and PBD SG-3199 activate dendritic cells (DCs) via a type-I IFN-dependent mechanism. However, STING deficiency in tumor cells does not prevent DC activation, suggesting that transactivation of the STING pathway occurs within DCs. Furthermore, depletion of the cytosolic DNA exonuclease TREX1 in tumor cells increases DC activation in response to PBD SG-3199-treated tumor cells, indicating that an increase in tumor-derived cytosolic DNA may further enhance DC activation. In summary, in this study, we show that ceralasertib and PBD SG-3199 treatment not only intrinsically target tumor cells but also extrinsically increase tumor cell immunogenicity by inducing DC activation, which is enhanced in ATM-deficient cells.