gebhart/patrepeval huggingface dataset:
The C terminus of the anaphylatoxin C3a generated upon complement activation represents a neoantigenic determinant with diagnostic potential. (1988)

Activation of the C component C3 results in generation of the anaphylatoxin C3a. The C3a polypeptide chain consists of 77 amino acids. The active site of this potent mediator, which also has immunoregulatory function resides in its C terminus. This report demonstrates that the C terminus of C3a (C3a-desArg) exposed by proteolytic cleavage from C3 represents a neoantigenic determinant. Two mAb specific for this epitope were obtained after immunization with the synthetic octapeptide (OP) Arg-Ala-Ser-His-Leu-Gly-Leu-Ala [C3a(69-76)] coupled to the carrier keyhole limpet hemocyanin (KLH). These anti-C3a(69-76) antibodies (H453 and H454) reacted in an ELISA system with C3a and KLH-OP but not with C3 or with KLH alone. Free OP efficiently blocked binding of the antibodies to C3a, whereas binding of another anti-C3a mAb (H13) remained unaffected. In immunoblotting analysis, the anti-C3a(69-76) mAb reacted with purified C3a but failed to react with the denatured, noncleaved C3. A novel quantitative C3a-ELISA was established with the anti-C3a(69-76) mAb. It had a sensitivity in the nanogram range (1 to 5 ng/ml). The C3a determination was not impaired by the presence of high concentrations of C3. Therefore, C3 removal was not required in contrast to the previously described C3a assays. This C3a ELISA might facilitate clinical C3a quantitation, e.g., in samples from patients with adult respiratory distress syndrome. In these patients, C3a determination in the early phase of the disease is of diagnostic relevance and has prognostic value.
The redox and DNA-repair activities of Ref-1 are encoded by nonoverlapping domains. (1994)

The DNA binding activity of transcription factor AP-1 is regulated in vitro by a posttranslational mechanism involving reduction/oxidation (redox). Redox regulation is mediated by a conserved cysteine residue in the DNA-binding domain of Fos and Jun. Previously, we demonstrated that a DNA repair protein, Ref-1, could stimulate the DNA binding activity of Fos-Jun dimers by reducing this cysteine residue. To examine the relationship between the redox and repair functions of Ref-1, we generated a series of deletion mutants. Analysis of the truncated proteins in vitro revealed that the redox and repair activities are encoded by distinct regions of Ref-1. Sequences in the N-terminal domain of Ref-1 that are not present in functionally related proteins from other organisms are required for the redox activity, whereas the DNA repair activity requires conserved C-terminal sequences. Chemical alkylation or oxidation of cysteine sulfhydryls inhibits the redox activity of Ref-1 without affecting its DNA repair activity. Crosslinking studies suggest that a direct cysteine-mediated interaction occurs between Ref-1 and Jun.
Review articles (1996)

Endothelial dysfunction is the final common pathway in the pathogenesis of preeclampsia. Future therapeutic modalities aimed at preventing or treating preeclampsia should either reduce the extent of (or even prevent) endothelial cell dysfunction (primary prevention) or should reduce the consequences of endothelial cell dysfunction (secondary and tertiary prevention). Relevant potential and promising directions for further research will be discussed. Part I of this review concerns the primary prevention of preeclampsia. The etiology of preeclampsia is still unknown. The 4 hypotheses that are the most popular currently are the placental ischemia hypothesis, the immune maladaptation/spiral artery-decidual ¿toxin' hypothesis, the VLDL (very low density lipoproteins)/TxPA (toxicity preventing activity) hypothesis, and the genetic hypothesis. For the near future only the disturbed VLDL/TxPA balance and the imbalance between free radicals and scavengers appear to be amenable for clinical research purposes.
Pharmacological and immunological characteristics of the therapeutic anti-EGFR antibodies cetuximab, panitumumab, and nimotuzumab. (2010)

e13025 Background: Therapeutic antibodies can affect tumor cells by functionally interfering with the target and/or by employing immune effector mechanisms such as antibody-dependent cellular cytotoxicity (ADCC). The scope of this study was to analyze the clinically used anti-EGFR therapeutic antibodies cetuximab, panitumumab, and nimotuzumab with regard to both modes of action: interference with EGFR function and ADCC activity. Methods: EGFR binding and inhibition of EGFR signal transduction were characterized in A431, A549, and HCT116 tumor cell lines. The ability to kill these tumor cells via ADCC was analyzed in ex vivo assays using human immune effector cells isolated from blood. Results: In cellular assays using A431, A549, and HCT116 cells cetuximab and panitumumab showed similar EGFR binding affinities (Kd values for binding to A431 cells of cetuximab and panitumumab: 5 nM and 4 nM, respectively) while nimotuzumab bound to EGFR with lower affinity (Kd for binding to A431 cells: ∼ 90 nM). The EGFR binding behavior correlated with the ability to inhibit EGFR signal transduction (phospho-EGFR, phospho-ERK1/2, phospho-Akt, phospho- STAT3) as well as inhibition of VEGF and IL-8 production. Using peripheral blood mononuclear cells (PBMCs) as effector cells, cetuximab mediated a marked, dose-dependent ADCC response in all three cell lines. Nimotuzumab displayed weak ADCC activity, while no or only marginal ADCC was observed with panitumumab. NK cells were the main effector cells of the PBMC fraction. With all three antibodies no ADCC activity was seen when polymymorphonuclear leukocytes were used as effector cells. Conclusions: Although cetuximab, panitumumab, and nimotuzumab are all clinically applied therapeutic antibodies directed against the EGFR, the results of this investigation suggest that different modes of action may contribute to the clinical efficacy of the antibodies. Author Disclosure Employment or Leadership Position Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration EMD Serono, Merck KGaA, Merck Serono
A phase I study of the natural killer T-cell ligand alpha-galactosylceramide (KRN7000) in patients with solid tumors. (2002)

i.v. KRN7000 is well tolerated in cancer patients over a wide range of doses. Biological effects were observed in several patients with relatively high pretreatment NKT-cell numbers. Other therapeutic strategies aiming at reconstitution of the deficient NKT-cell population in cancer patients may be warranted.
Nanosecond switching of thin ferroelectric films (1991)

The switching time (ts) for polarization reversal in ferroelectric films of PbZr0.53Ti0.47O3 and La-substituted PbTiO3 has been investigated. The films were prepared by spin-on and metalorganic decomposition followed by processing into ferroelectric capacitors having electrode areas ranging from 9 to 50 000 μm2. Pulse measurements show that under all our experimental conditions ts is instrumentally limited and that therefore the true switching time is smaller than the experimental resolution of 1.8 ns. This very fast polarization reversal can be explained by a nucleation-rate-controlled switching mechanism.
Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens. (1990)

Regulation of proliferation of normal human T lymphocytes (T cells) by glutathione (GSH) was explored with T-cell activation models that do not require accessory cell signals. L-Buthionine-(S,R)-sulfoximine (BSO), which inactivates gamma-glutamylcysteine synthetase and therefore inhibits GSH synthesis, inhibited proliferation elicited by monoclonal antibodies directed at cluster designation 2 (CD2) and CD3 antigens, or by sn-1,2-dioctanoylglycerol and ionomycin. L-Buthionine-(R)-sulfoximine, which does not inactivate gamma-glutamylcysteine synthetase, did not affect proliferation. BSO-induced inhibition of accessory cell-independent T-cell proliferation was not reversed by recombinant human interleukin 2, despite activation-dependent expression of interleukin 2 receptor alpha by T cells treated with BSO. However, BSO-associated inhibition of T-cell proliferation was reversed by GSH or GSH ester. These studies, which show that GSH can directly modulate proliferation of highly purified T cells, suggest that GSH is essential for steps close to or at DNA synthesis. The availability of methods for decreasing and for increasing GSH levels suggest therapies to produce (i) immunosuppression (of value in organ transplantation), and (ii) immunopotentiation (of potential value in treatment of immunodeficiency states such as AIDS).
Periodic Leg Movements (PLM): Their Relationship to Sleep Stages (1993)

We investigated the characteristics of periodic leg movements (PLM) during nocturnal sleep and wakefulness in 13 drug-free patients presenting with the restless legs syndrome (RLS, n = 9) or with isolated PLM (n = 4). Eight-hour polygraphic sleep recordings included the electromyogram (EMG) of both tibialis anterior muscles. Scoring of leg movements was done according to established criteria for periodic movements in sleep, but movements occurring during episodes of wakefulness were scored as well. Twelve out of 13 patients had PLM during wakefulness, including three subjects not affected by RLS. The frequency of periodic movements in sleep (PMS) per hour of total sleep time was significantly lower than the frequency of PLM (including movements during wakefulness) per hour of polygraphic recording. Movement indices based on PMS alone underestimated the relative frequency of PLM particularly in patients with high amounts of wakefulness (> 20%). All features of PLM clearly differed between sleep stages. Relative frequency of movements, their duration and their arousing effect decreased along the nonrapid eye movement (NREM) sleep stages, whereas the intermovement interval increased. During rapid eye movement (REM) sleep the duration of movements was shortest and the intermovement interval was longest. The results presented suggest that the processes underlying PLM are most active at the transition from wakefulness to sleep and considerably attenuated during deep NREM sleep and even more during REM sleep. We suggest including movements during wakefulness in routine PLM scoring to get a more complete picture of the disturbance.
On the complexity of VLSI implementations and graph representations of Boolean functions with application to integer multiplication (1991)

Lower-bound results on Boolean-function complexity under two different models are discussed. The first is an abstraction of tradeoffs between chip area and speed in very-large-scale-integrated (VLSI) circuits. The second is the ordered binary decision diagram (OBDD) representation used as a data structure for symbolically representing and manipulating Boolean functions. The lower bounds demonstrate the fundamental limitations of VLSI as an implementation medium, and that of the OBDD as a data structure. It is shown that the same technique used to prove that any VLSI implementation of a single output Boolean function has area-time complexity AT/sup 2/= Omega (n/sup 2/) also proves that any OBDD representation of the function has Omega (c/sup n/) vertices for some c>1 but that the converse is not true. An integer multiplier for word size n with outputs numbered 0 (least significant) through 2n-1 (most significant) is described. For the Boolean function representing either output i-1 or output 2n-i-1, where 1<or=i<or=n, the following lower bounds are proved: any VLSI implementation must have AT/sup 2/= Omega (i/sup 2/) and any OBDD representation must have Omega (1.09/sup i/) vertices.<<ETX>>
The rsmA ‐like gene rsmA Xoo of Xanthomonas oryzae pv. oryzae regulates bacterial virulence and production of diffusible signal factor (2010)

The plant-pathogenic prokaryote Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight, one of the most destructive diseases of rice. A nonpolar mutant of the rsmA-like gene rsmA(Xoo) of the Xoo Chinese strain 13751 was constructed by homologous integration with a suicide plasmid. Virulence tests on a host plant, namely the hybrid rice cultivar Teyou 63, showed that the mutant had lost its virulence almost completely, whereas tests on a nonhost, namely castor-oil plant (Ricinus communis), showed that the mutant had also lost the ability to induce a hypersensitive response in the nonhost. In addition, the rsmA(Xoo) mutant produced significantly smaller amounts of the diffusible signal factor, extracellular endoglucanase, amylase and extracellular polysaccharide, but showed significantly higher glycogen accumulation, bacterial aggregation and cell adhesion. The expression of most hrp genes, genes encoding AvrBs3/PthA family members, rpfB, xrvA, glgA, eglXoB and XOO0175 (encoding an α-amylase) was down-regulated in the rsmA(Xoo) mutant. All phenotypes and expression levels of the tested genes in the rsmA(Xoo) mutant were restored to their levels in the wild-type by the presence of rsmA(Xoo) in trans. These results indicate that rsmA(Xoo) is essential for the virulence of Xoo.
Multiple monoclonal B cell expansions and c-myc oncogene rearrangements in acquired immune deficiency syndrome-related lymphoproliferative disorders. Implications for lymphomagenesis. (1986)

AIDS (acquired immune deficiency syndrome) and ARC (AIDS-related complex) are associated with a spectrum of lymphoproliferative disorders ranging from lymphadenopathy syndrome (LAS), an apparently benign polyclonal lymphoid hyperplasia, to B cell non-Hodgkin's lymphoma (B-NHL), i.e., malignant, presumably monoclonal B cell proliferations. To gain insight into the process of lymphomagenesis in AIDS and to investigate a possible pathogenetic relationship between LAS and NHL, we investigated the clonality of the B or T lymphoid populations by Ig or T beta gene rearrangement analysis, the presence of rearrangements involving the c-myc oncogene locus, and the presence of human immunodeficiency virus (HIV) sequences in both LAS and B-NHL biopsies. Our data indicate that multiple clonal B cell expansions are present in a significant percentage of LAS (approximately 20%) and B-NHL (60%) biopsies. c-myc rearrangements/translocations are detectable in 9 of our 10 NHLs, but not in any of the LAS cases. However, only one of the B cell clones, identified by Ig gene rearrangements carries a c-myc gene rearrangement, suggesting that only one clone carries the genetic abnormality associated with malignant B cell lymphoma. Furthermore, the frequency of detection of c-myc rearrangements in AIDS-associated NHLs of both Burkitt and non-Burkitt type suggest that the biological alterations present in AIDS favor the development of lymphomas carrying activated c-myc oncogenes. Finally, our data show that HIV DNA sequences are not detectable in LAS nor in NHL B cell clones, suggesting that HIV does not play a direct role in NHL development. Taken together, these observations suggest a model of multistep lymphomagenesis in AIDS in which LAS would represent a predisposing condition to NHL. Immunosuppression and EBV infection present in LAS can favor the expansion of B cell clones, which in turn may increase the probability of occurrence of c-myc rearrangements leading to malignant transformation.
Estimated Annual Costs of Campylobacter-Associated Guillain-Barre Syndrome (1997)

Guillain-Barre syndrome (GBS) is an autoimmune reaction that can cause acute neuro-muscular paralysis. Of an estimated 2,628 to 9,575 new U.S. cases with GBS annually, 526 to 3,830 are triggered by infection with Campylobacter, the most frequently isolated cause of foodborne diarrhea. Estimated total annual costs of Campylobacter-associated GBS of $0.2 to $1.8 billion plus previously estimated costs of campylobacteriosis ($1.3 to $6.2 billion) add to total annual costs from Campylobacter of $1.5 to $8.0 billion (1995 dollars). Assuming 55-70 percent of costs are attributable to foodborne sources, costs of campylobacteriosis from food sources ($0.7 to $4.3 billion) and costs of associated GBS ($0.1 to $1.3 billion) combined equal total annual costs of $0.8 to $5.6 billion from foodborne Campylobacter. Reducing Campylobacter in food could prevent up to $5.6 billion in costs annually.
The Modulation Transfer Function in Room Acoustics as a Predictor of Speech Intelligibility (1973)

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Overview of the Advanced Camera for Surveys on-orbit performance (2003)

We present an overview of the ACS on-orbit performance based on the calibration observations taken during the first three months of ACS operations. The ACS meets or exceeds all of its important performance specifications. The WFC and HRC FWHM and 50% encircled energy diameters at 555 nm are 0.088" and 0.14", and 0.050" and 0.10". The average rms WFC and HRC read noises are 5.0 e<sup>-</sup> and 4.7 e<sup>-</sup>. The WFC and HRC average dark currents are ~ 7.5 and ~ 9.1 e<sup>-</sup>/pixel/hour at their operating temperatures of - 76°C and - 80°C. The SBC + HST throughput is 0.0476 and 0.0292 through the F125LP and F150LP filters. The lower than expected SBC operating temperature of 15 to 27°C gives a dark current of 0.038 e<sup>-</sup>/pix/hour. The SBC just misses its image specification with an observed 50% encircled energy diameter of 0.24" at 121.6 nm. The ACS HRC coronagraph provides a 6 to 16 direct reduction of a stellar PSF, and a ~1000 to ~9000 PSF-subtracted reduction, depending on the size of the coronagraphic spot and the wavelength. The ACS grism has a position dependent dispersion with an average value of 3.95 nm/pixel. The average resolution &#955;/&#916;&#955; for stellar sources is 65, 87, and 78 at wavelengths of 594 nm, 802 nm, and 978 nm.
Light Field Photography with a Hand-held Plenoptic Camera (2005)

This paper presents a camera that samples the 4D light field on its sensor in a single photographic exposure. This is achieved by inserting a microlens array between the sensor and main lens, creating a plenoptic camera. Each microlens measures not just the total amount of light deposited at that location, but how much light arrives along each ray. By resorting the measured rays of light to where they would have terminated in slightly different, synthetic cameras, we can compute sharp photographs focused at different depths. We show that a linear increase in the resolution of images under each microlens results in a linear increase in the sharpness of the refocused photographs. This property allows us to extend the depth of field of the camera without reducing the aperture, enabling shorter exposures and lower image noise. Especially in the macrophotography regime, we demonstrate that we can also compute synthetic photographs from a range of different viewpoints. These capabilities argue for a different strategy in designing photographic imaging systems. To the photographer, the plenoptic camera operates exactly like an ordinary hand-held camera. We have used our prototype to take hundreds of light field photographs, and we present examples of portraits, high-speed action and macro close-ups.
New attachment following surgical treatment of human periodontal disease (1982)

The present experiment was undertaken to test the hypothesis that new connective tissue attachment may form on a previously periodontitis involved root surface provided cells originating from the periodontal ligament are enabled to repopulate the root surface during healing. A mandibular incisor with advanced periodontal disease of long standing (the distance between the cemento-enamel junction and the alveolar bone crest was 9 mm) was subjected to periodontal surgery using a technique which during healing prevented the dentogingival epithelium and the gingival connective tissue from reaching contact with the curetted root surface. Preference was hereby given to the periodontal ligament cells to repopulate the previously diseased root surface. After 3 months of healing a block biopsy containing the incisor and surrounding tissue was sampled. The histological analysis revealed that new cementum with inserting principal fibers had formed on the previously diseased root surface. This new attachment extended in coronal direction to a level 5 mm coronal to the alveolar bone crest. This finding suggests that new attachment can be achieved by cells originating from the periodontal ligament and demonstrates that the concept that the periodontitis affected root surface is a major preventive factor for new attachment is invalid.
Consideraciones sobre el estudio de catálisis homogénea y heterogénea (2005)

"El propósito de este trabajo es brindar información sobre catálisis, su fundamento y algunas consideraciones esenciales sobre el empleo de la catálisis homogénea y heterogénea en nuestros tiempos, así como ventajas y diferencias a nivel industrial."
n–3 Fatty Acids and Cardiovascular Outcomes in Patients with Dysglycemia (2012)

Daily supplementation with 1 g of n-3 fatty acids did not reduce the rate of cardiovascular events in patients at high risk for cardiovascular events. (Funded by Sanofi; ORIGIN ClinicalTrials.gov number, NCT00069784.).
Reversible Inactivation of Protein-tyrosine Phosphatase 1B in A431 Cells Stimulated with Epidermal Growth Factor (1998)

Stimulation of various cells with growth factors results in a transient increase in the intracellular concentration of H2O2 that is required for growth factor-induced protein tyrosine phosphorylation. The effect of H2O2 produced in response to epidermal growth factor (EGF) on the activity of protein-tyrosine phosphatase 1B (PTP1B) was investigated in A431 human epidermoid carcinoma cells. H2O2 inactivated recombinant PTP1B in vitro by oxidizing its catalytic site cysteine, most likely to sulfenic acid. The oxidized enzyme was reactivated more effectively by thioredoxin than by glutaredoxin or glutathione at their physiological concentrations. Oxidation by H2O2 prevented modification of the catalytic cysteine of PTP1B by iodoacetic acid, suggesting that it should be possible to monitor the oxidation state of PTP1B in cells by measuring the incorporation of radioactivity into the enzyme after lysis of the cells in the presence of radiolabeled iodoacetic acid. The amount of such radioactivity associated with PTP1B immunoprecipitated from A431 cells that had been stimulated with EGF for 10 min was 27% less than that associated with PTP1B from unstimulated cells. The amount of iodoacetic acid-derived radioactivity associated with PTP1B reached a minimum 10 min after stimulation of cells with EGF and returned to base line values by 40 min, suggesting that the oxidation of PTP1B is reversible in cells. These results indicate that the activation of a receptor tyrosine kinase by binding of the corresponding growth factor may not be sufficient to increase the steady state level of protein tyrosine phosphorylation in cells and that concurrent inhibition of protein-tyrosine phosphatases by H2O2 might also be required.
T-helper (1) cytokines increase during early pregnancy in women with a history of recurrent spontaneous abortion. (2002)

These data may explain the increase NK-cells cytolytic activity during early pregnancy in RSA group. It may also provide a diagnostic tool to predict the outcome of pregnancy.