gebhart/patrepeval huggingface dataset:
Resolution of Longstanding Symptoms of Multiple Sclerosis by Application of Picotesla Range Magnetic Fields (1993)

Recent clinical reports have suggested that treatment with extremely weak magnetic fields (MF) in the picoTesla range is an efficacious modality for the symptomatic therapy in patients with multiple sclerosis (MS) during the remission and exacerbation periods of the disease. The present communication concerns a 64 year old woman with a 22 year history of MS of the chronic-progressive type who presented with a longstanding history of ataxia of gait, weakness in the legs, difficulties with swallowing, loss of bladder control, blurred vision, diplopia, chronic fatigue, and cognitive impairment. In this patient two 30 minute treatments with MF on two separate days resulted in a dramatic improvement of symptoms. Specifically, the patient experienced marked improvement in balance and gait as well as increased strength in the legs to the extent that she was able to abandon the use of a walker within 48 hours after initiation of magnetic treatment. In addition, there was complete resolution of diplopia, bladder dysfunction, and fatigue with improvement in mood and cognitive functions. The report attests to the unique efficacy of extremely weak MF in the symptomatic treatment of patients with MS including those patients with a chronic progressive course of the disease and supports the hypothesis that dysfunction of synaptic conductivity due to neurotransmitter deficiency specifically of serotonin rather than demyelination underlies the neurologic deficits of the disease.
Dense surface models of the human face (2004)

This thesis describes and evaluates Dense Surface Models (DSMs), a new technique for building point distribution models of surfaces, from raw input data. DSMs can be used on data from a wide range of surface acquisition systems without preprocessing since they do not require that the surfaces be closed or even locally manifold, and can cope well with holes and spikes in the surfaces. This is an advantage over comparable techniques, which impose such constraints on the input. The core of the DSM algorithm is as follows. Adense correspondence is made between the surfaces using thin-plate spline warping guided by means of a small set of hand-placed landmarks. The area of interest is automatically defined by a threshold on a measure of the closeness of the correspondence at each point. Apoint distribution model is then built using the vertices from the trimmed and densely-corresponded surfaces. The key benefit of using models of the whole surface is illustrated by the large improvement in classification on face shape that is obtained when using DSMs as compared
Nucleotide sequence of the Clostridium acidiurici ("Clostridium acidi-urici") gene for 10-formyltetrahydrofolate synthetase shows extensive amino acid homology with the trifunctional enzyme C1-tetrahydrofolate synthase from Saccharomyces cerevisiae (1988)

The nucleotide sequence of the gene for 10-formyltetrahydrofolate synthetase (EC 6.3.4.3) from Clostridium acidiurici ("Clostridium acidi-urici") was determined. The synthetase mRNA initiation and termination regions were determined by primer extension and S1 nuclease mapping. Two potential -10 and -35 promoter regions were identified upstream of mRNA initiation. The terminator region was found to be in a large region of dyad symmetry. A comparison of the amino acid sequences of the monofunctional synthetase and the eucaryotic trifunctional enzyme, C1-tetrahydrofolate synthase, from Saccharomyces cerevisiae demonstrated a region of strong homology.
Isolation and characterization of cell surface mutants of Candida albicans (1990)

Mutant strains of Candida albicans were obtained by selecting for cells that escaped agglutination by a polyclonal antiserum raised against standard C. albicans serotype A isolate B311. Mutants were obtained from strains B311 and B792 and from four strains isolated from patients with acquired immunodeficiency syndrome. All 15 tested mutants retained characteristic sugar assimilation patterns. All but one of the mutants retained the ability to form germ tubes and chlamydospores. Two mutants from an acquired immunodeficiency syndrome-derived isolate were deficient in binding complement ligands iC3b and C3d, whereas another mutant was deficient in binding ligand iC3b but not C3d. The hyphae of these three mutants lacked antigens when examined by Western immunoblotting with monoclonal antibody Ca-A, which detects several glycoproteins, including C3d-binding proteins. One of the complement-binding-deficient mutants was tested for its ability to colonize the gastrointestinal tract of rabbits but did not differ from the wild-type parent in site or degree of colonization. The proton magnetic resonance spectra of bulk mannan carbohydrate extracted from tested mutants showed the loss of a signal characteristic of the mannosyl alpha-PO4 linkage; each mutant also had a distinct pattern of other changes.
Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes (2008)

A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.
Photoacoustic tomography in absorbing acoustic media using time reversal (2010)

The reconstruction of photoacoustic images typically neglects the effect of acoustic absorption on the measured time domain signals. Here, a method to compensate for acoustic absorption in photoacoustic tomography is described. The approach is based on time-reversal image reconstruction and an absorbing equation of state which separately accounts for acoustic absorption and dispersion following a frequency power law. Absorption compensation in the inverse problem is achieved by reversing the absorption proportionality coefficient in sign but leaving the equivalent dispersion parameter unchanged. The reconstruction is regularized by filtering the absorption and dispersion terms in the spatial frequency domain using a Tukey window. This maintains the correct frequency dependence of these parameters within the filter pass band. The method is valid in one, two and three dimensions, and for arbitrary power law absorption parameters. The approach is verified through several numerical experiments. The reconstruction of a carbon fibre phantom and the vasculature in the abdomen of a mouse are also presented. When absorption compensation is included, a general improvement in the image magnitude and resolution is seen, particularly for deeper features. 1.
Stable Expression of the Hepatitis B Virus Surface Antigen Containing Pre-S2 Protein in Mouse Cells Using a Bovine Papillomavirus Vector (1988)

The large BglII fragment (2.8 kilobases) of hepatitis B virus DNA including the transcription unit for the hepatitis B surface antigen (HBsAg) was inserted into a bovine papillomavirus vector containing the neomycin resistance gene. The recombinant DNA was transfected into mouse C127 cells. A stable transformed cell line (MS128) secreting a large amount of 22 nm HBsAg particles containing pre-S2 protein was established. The secreted HBsAg particles had the receptor for polymerized human serum albumin. Immunoprecipitation and Western blot analyses showed that HBsAg particles consisted of two major proteins of 22K and 26K encoded by the S gene and a minor protein of 35K encoded by the pre-S2 and S genes. Southern blot analysis revealed that the transfected plasmid was integrated into the host chromosomal DNA and that most of the plasmid sequences were present. These results suggest that the stable expression of the HBsAg in MS128 cells is related to the integrated state of the recombinant DNA.
Passaging Protocols for Mammalian Neural Stem Cells in Suspension Bioreactors (2002)

Mammalian neural stem cells (NSC) offer great promise as therapeutic agents for the treatment of central nervous system disorders. As a consequence of the large numbers of cells that will be needed for drug testing and transplantation studies, it is necessary to develop protocols for the large-scale expansion of mammalian NSC. Neural stem cells and early progenitor cells can be expanded in vitro as aggregates in controlled bioreactors using carefully designed media. The first objective of this study was to determine if it is possible to maintain a population of murine neural stem and progenitor cells as aggregates in suspension culture bioreactors over extended periods of time. We discovered that serial passaging of a mixture of aggregates sizes resulted in high viabilities, high viable cell densities, and good control of aggregate diameter. When the NSC aggregates were serially subcultured three times without mechanical dissociation, a total multiplication ratio of 2.9 x 10(3) was achieved over a period of 12 days, whereas the aggregate size was controlled (mean diameter less than 150 microm) below levels at which necrosis would occur. Moreover, cell densities of 1.0 x 10(6) cells/mL were repeatedly achieved in batch culture with viabilities exceeding 80%. The second objective was to examine the proliferative potential of single cells shed from the surface of these aggregates. We found that the single cells, when subcultured, retained the capacity to generate new aggregates, gave rise to cultures with high viable cell densities and were able to differentiate into all of the primary cell phenotypes in the central nervous system.
Correlation between nanostructural parameters and conductivity properties for molecularly-mediated thin film assemblies of gold nanoparticles (2006)

The understanding of the particle size and interparticle spatial effects on the electrical conductivity properties of molecularly mediated thin film assemblies of nanoparticles is important for exploring the unique electrical properties in applications such as chemical sensors and biosensors. This paper reports findings of an investigation of such effects for thin film assemblies of gold nanoparticles of highly-monodispersed sizes (2–4 nm) using alkyl dithiols of different chain lengths (0.8–2 nm). The conductivities of the thin films were measured using interdigitated microelectrodes as the platform. Experimental results have shown that the activation energy increases with chain length and decreases with particle size, and the electron tunneling decay term decreases with particle size. The results have revealed that the experimentally determined conductivity and activation energy data for the nanoparticle films quantitatively match the calculations from electrostatic model of granular metals through the electron tunneling mechanism. The strong correlation between the experimental and calculated data was attributed to a combination of the high monodispersity of the nanoparticles and the uniformity and stability of the thin film assemblies. These findings have provided an important set of systematic data supporting the applicability of the activated electron tunneling theory to the molecularly-mediated thin film assemblies of gold nanoparticles, which have important implications for the design and fine-tuning of nanostructured thin films as chemical and biological sensing materials.
Kinins contribute to the improvement of insulin sensitivity during treatment with angiotensin converting enzyme inhibitor. (1994)

Although angiotensin converting enzyme inhibitors and alpha 1-blockers have been reported to improve insulin sensitivity, their mechanisms of action have not been elucidated. To investigate the role of kinins in insulin sensitivity, we treated 4-week-old spontaneously hypertensive rats with either an angiotensin converting enzyme inhibitor (enalapril), an alpha 1-blocker (doxazosin), or an angiotensin II antagonist (losartan) for 3 weeks. A control group received no drugs. In addition, 18 rats treated with enalapril or doxazosin received a simultaneous administration of a kinin antagonist (Hoe 140). Glucose clamp testing was performed in each group. Enalapril (128 +/- 1 mmHg) and doxazosin (132 +/- 2 mmHg) decreased mean blood pressure compared with control levels (148 +/- 1 mmHg) (P &lt; .01). The glucose requirement for the clamp test during the administration of enalapril (25.8 +/- 0.5 mg/kg per minute) or doxazosin (28.6 +/- 0.7 mg/kg per minute) was higher than that of the control group (19.8 +/- 0.5 mg/kg per minute) (P &lt; .05). Although Hoe 140 did not alter the glucose requirement of doxazosin (27.8 +/- 0.5 mg/kg per minute), it decreased that of enalapril (22.6 +/- 0.9 mg/kg per minute) (P &lt; .05) without affecting the changes in mean blood pressure induced by enalapril. In addition, losartan decreased mean blood pressure but did not affect the glucose requirement. Thus, the improvement in insulin sensitivity produced by an angiotensin converting enzyme inhibitor is mostly dependent on kinins but not on angiotensin II antagonism, and an alpha 1-blocker improves insulin sensitivity irrespective of kinins.
Expression of ER, PR, and Her-2/neu in endometrial cancer: a clinicopathological study. (2010)

ER expression is a good prognostic factor while Her-2/neu expression appears to be a poor indicator for both disease-free and overall survival, while PR tended to show favorable influence for only disease-free survival of Thai EMCs.
Optimized high-throughput microRNA expression profiling provides novel biomarker assessment of clinical prostate and breast cancer biopsies. (2006)

In sum, these findings demonstrate that optimized high-throughput microRNA expression profiling offers novel biomarker identification from typically small clinical samples such as breast and prostate cancer biopsies.
In vitro activities of two novel oxazolidinones (U100592 and U100766), a new fluoroquinolone (trovafloxacin), and dalfopristin-quinupristin against Staphylococcus aureus and Staphylococcus epidermidis (1996)

Two oxazolidinones (U100592 and U100766), trovafloxacin, and a streptogramin combination (dalfopristin-quinupristin) were highly active in vitro against Staphylococcus aureus and Staphylococcus epidermidis, including methicillin-resistant strains. Trovafloxacin was more active than ciprofloxacin. Time-kill synergy studies demonstrated indifference for the oxazolidinones combined with vancomycin and rifampin against methicillin-resistant staphylococci. Spontaneous resistance was observed with all agents.
Preoperative Evaluation of Hilar Cholangiocarcinoma with Contrast‐enhanced Three‐dimensional Fast Imaging with Steady‐state Precession Magnetic Resonance Angiography: Comparison with Intraarterial Digital Subtraction Angiography (2003)

We sought to evaluate the image quality of double-dose, contrast-enhanced 3D fast imaging with steady-state precession (FISP) magnetic resonance (MR) angiography in patients with hilar cholangiocarcinoma, and to compare its efficacy with intraarterial digital subtraction angiography (DSA). Thirty-six patients were studied to determine the visibility of the hepatic artery and portal vein with contrast-enhanced MR angiography. Determination of hepatic arterial and/or portal invasion from cholangiocarcinoma was compared between MR angiography and DSA as well. 0.2 mmol/kg gadolinium chelates were administrated at a rate of 4 ml/s. The hepatic artery was diagnostically visible in 28 patients (78%); the portal vein, in 34 patients (94%). The sensitivities, specificities, and accuracies in distinguishing arterial invasion, were 58%, 93%, and 89%, respectively, with MR angiography; 75%, 99%, and 96%, respectively, with DSA. In distinguishing portal venous invasion, these were 78%, 91%, and 89%, respectively, with MR angiography; 78%, 92%, and 90%, respectively, with DSA. There were no significant differences between imaging methods ( p >.05); however, DSA was superior in specificity regarding hepatic arterial invasion ( p =.0143). The data presented here indicate that noninvasive contrast-enhanced 3D FISP MR angiography has the potential to replace DSA in the preoperative evaluation of hilar cholangiocarcinoma.
A communication-induced checkpointing protocol that ensures rollback-dependency trackability (2002)

Considering an application in which processes take local checkpoints independently (called basic checkpoints), this paper develops a protocol that forces them to take some additional local checkpoints (called forced checkpoints) in order that the resulting checkpoint and communication pattern satisfies the Rollback Dependency Trackability (RDT) property. This property states that all dependencies between local checkpoints are on-line trackable by using a transitive dependency vector. Compared to other protocols ensuring the RDT property, the proposed protocol is less conservative in the sense that it takes less additional local checkpoints. It attains this goal by a subtle tracking of causal dependencies on already taken checkpoints; this tracking is then used to prevent the occurrence of hidden dependencies. As indicated by simulation study, the proposed protocol compares favorably with other protocols; moreover it additionally associates on-the-fly with each local checkpoint C the minimum global checkpoint to which C belongs.
Screening forStaphylococcus epidermidisMarkers Discriminating between Skin‐Flora Strains and Those Responsible for Infections of Joint Prostheses (2000)

Fifty-four Staphylococcus epidermidis strains responsible for infections of joint prostheses and 23 strains isolated from skin flora were studied for markers of virulence, to discriminate invasive strains from normal flora. They were screened for binding to polystyrene and matrix proteins and for the presence of staphylococcal genes involved in adhesion. The ica operon involved in biofilm formation was the only marker discriminating between these 2 categories of strains.
Rome III: The Functional Gastrointestinal Disorders (2006)

When it was first convened in 1990, the Rome committee sought to bring order to the largely amorphous world of functional gastrointestinal disorders (FGIDs). Sixteen years later, in the third edition of the Rome criteria, the authors continue their quest to update and refine the classification of FGIDs. The first 6 chapters are dedicated to neurogastroenterology, pharmacology, the role of gender and culture, and the psychologic aspects of FGIDs. Although a basic understanding of these aspects of FGIDs can be helpful, the level of detail in a few of these initial chapters (ie, the fundamentals of neurogastroenterology) is more than is necessary for the average clinician. As in past issues, the discussion of FGIDs is organized by organ system. Each disorder’s criteria are clearly listed at the beginning of the chapter followed by a detailed discussion of appropriate diagnostic and therapeutic strategies. Salient studies are succinctly summarized when appropriate, allowing clinicians the opportunity to quickly reference supporting data. Rome III does include several important changes to the previous edition. The time frame criteria required for FGID diagnosis has been simplified, several new classification categories have been created (mainly in the realm of pediatric FGIDs), and diagnostic criteria have been amended for functional dyspepsia and irritable bowel syndrome. Rome III is clearly written and well organized. Although the book’s size and subject matter may at first seem overwhelming, its excellent use of tables and diagnostic flow charts allows for easy application to medical practice. The patient questionnaires and scoring algorithms contained in the appendix are arguably the most useful to the practicing clinician. Also highlighted in this section is the specific testing necessary to exclude the presence of underlying structural disease before diagnosis of a particular FGID. Recognizing the inherent difficulty in adopting new guidelines, the authors even include the old Rome II criteria side-by-side with the updated criteria. Recommendation: In all, the authors of Rome III have done an excellent job of creating a resource on FGIDs that is comprehensive and detailed while also being clinically relevant and easy-to-use. A must have for any practicing gastroenterologist.
Use of a Patterned Self-Assembled Monolayer To Control the Formation of a Liquid Resist Pattern on a Gold Surface (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of a Patterned Self-Assembled Monolayer To Control the Formation of a Liquid Resist Pattern on a Gold SurfaceChristopher B. Gorman, Hans A. Biebuyck, and George M. WhitesidesCite this: Chem. Mater. 1995, 7, 2, 252–254Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://pubs.acs.org/doi/10.1021/cm00050a003https://doi.org/10.1021/cm00050a003research-articleACS PublicationsRequest reuse permissionsArticle Views246Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The non-histone proteins of the rat liver nucleus and their distribution amongst chromatin fractions as produced by nuclease digestion (1979)

The search for proteins involved in maintaining higher order chromatin structures has led to a systematic examination of the non-histone proteins (NHP) of rat liver nuclei in the context of nuclease digestion studies. 40-45% of the 3H-tryptophan labelled NHP originally present could be removed by extensive washing in a "physiological" buffer, incubation at 37 degrees C with or without nuclease and a further wash step. Nuclei at this stage had a remarkably constant NHP content (ca. 0.73 micrograms/micrograms DNA), independent of the degree of digestion with micrococcal nuclease or HaeIII. The solubilized chromatin produced by limited digestion with either nuclease contained 0.3-0.5 microgram NHP/microgram DNA, this value falling to ca. 0.16 after more extensive cleavage. Insoluble chromatin fractions were between 2-fold (very limited digestion) and 16-fold (extensive digestion) richer in NHP than the corresponding soluble fractions. Gel electrophoresis revealed about 12 NHP bands in soluble fractions, the most prominent of M.Wt. 41.400, while the insoluble material had at least 50 components. These properties were independent of whether lysis of nuclei occurred in 0.2 or 50 mM ionic strength. The large disparity in NHP content between complementary soluble and insoluble chromatin fractions is considered in terms of chromatin organization in vivo and the possible role of NHP migration.