gebhart/patrepeval huggingface dataset:
A large-area strain sensing technology for monitoring fatigue cracks in steel bridges (2017)

This paper presents a novel large-area strain sensing technology for monitoring fatigue cracks in steel bridges. The technology is based on a soft elastomeric capacitor (SEC), which serves as a flexible and large-area strain gauge. Previous experiments have verified the SEC's capability to monitor low-cycle fatigue cracks experiencing large plastic deformation and large crack opening. Here an investigation into further extending the SEC's capability for long-term monitoring of fatigue cracks in steel bridges subject to traffic loading, which experience smaller crack openings. It is proposed that the peak-to-peak amplitude (pk–pk amplitude) of the sensor's capacitance measurement as the indicator of crack growth to achieve robustness against capacitance drift during long-term monitoring. Then a robust crack monitoring algorithm is developed to reliably identify the level of pk–pk amplitudes through frequency analysis, from which a crack growth index (CGI) is obtained for monitoring fatigue crack growth under various loading conditions. To generate representative fatigue cracks in a laboratory, loading protocols were designed based on constant ranges of stress intensity to limit plastic deformations at the crack tip. A series of small-scale fatigue tests were performed under the designed loading protocols with various stress intensity ratios. Test results under the realistic fatigue crack conditions demonstrated the proposed crack monitoring algorithm can generate robust CGIs which are positively correlated with crack lengths and independent from loading conditions.
Protein Binding Has a Large Effect on Radical Mediated DNA Damage (2008)

Oxidative DNA damage is important in aging and a variety of diseases. Significant advances have been made in our understanding of the chemistry of radical mediated DNA damage. These studies have been carried out on DNA in the absence of proteins. However, in cells DNA is typically bound by proteins such as in chromatin and transiently by proteins that regulate biochemical processes. How and whether protein binding affects DNA radical reactivity is not well understood. The effect of the DNA binding protein Hbb on the reactivity of the 5-(2'-deoxyuridinyl)methyl radical (1) and 5-(2'-deoxycytidinyl)methyl radical (2) was studied. Hbb bends DNA and disrupts base stacking at the sites of kinking. The reactivity of 1 and 2 are significantly affected when they are generated at the kinking site in the presence of Hbb. The increased conformational mobility of the radicals results in significantly higher yields of DNA interstrand cross-links. These studies provide the first specific data on how protein binding affects the reactivity of a DNA radical and bring us closer to understanding oxidative DNA damage in cells.
Dendritic Cells from Mice Neonatally Vaccinated with Modified Vaccinia Virus Ankara Transfer Resistance against Herpes Simplex Virus Type I to Naive One-Week-Old Mice (2004)

Modified vaccinia Ankara (MVA) is an attenuated virus. MVA induces the production of IFN and Flt3-L (FL), which results in the expansion of dendritic cells (DC) and enhanced resistance against viral infections. We report on the interplay among IFN, FL, and DC in the resistance against heterologous virus after injection of neonatal mice with MVA. The induction of serum FL was tested on day 2, and the expansion of DC was tested 1 wk after treatment with MVA. At this time point the resistance against infection with heterologous virus was also determined. After MVA treatment, serum FL was enhanced, and DC, including plasmacytoid cells in spleen, were increased in number. Mice that lacked functional IFN type I and II systems failed to increase both the concentration of FL and the number of DC. Treatment with MVA enhanced resistance against HSV-1 in wild-type animals 100-fold, but animals without a functional IFN system were not protected. Transfer of CD11c(+) cells from MVA-treated mice into naive animals protected against lethal infection with HSV-1. Thus, although the increased resistance could be largely attributed to the increase in activation of IFN-producing plasmacytoid cells, this, in turn, depends on a complex interplay between the DC and T cell systems involving both FL and IFNs.
Benzaldehyde as a Carcinostatic Principle in Figs (1978)

Journal Article Benzaldehyde as a Carcinostatic Principle in Figs Get access Setsuo Takeuchi, Setsuo Takeuchi The Institute of Physical and Chemical Research, Wako-Shi, Saitama-ken Search for other works by this author on: Oxford Academic Google Scholar Mutsuyuki Kochi, Mutsuyuki Kochi lchijo-Kai Hospital, 4-26-1, Kita-kokubn, Ichikawa-shi, Chiba-ken Search for other works by this author on: Oxford Academic Google Scholar Kenji Sakaguchi, Kenji Sakaguchi Mitsubishi-Kasei Institute of Life Sciences, Minamiooya, Machida-shi, Tokyo Search for other works by this author on: Oxford Academic Google Scholar Kuniko Nakagawa, Kuniko Nakagawa The Institute of Physical and Chemical Research, Wako-Shi, Saitama-ken Search for other works by this author on: Oxford Academic Google Scholar Takeo Mizutani Takeo Mizutani The Institute of Physical and Chemical Research, Wako-Shi, Saitama-ken Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 42, Issue 7, 1 July 1978, Pages 1449–1451, https://doi.org/10.1080/00021369.1978.10863185 Published: 01 July 1978 Article history Received: 12 April 1978 Published: 01 July 1978
Application of neural networks in spatio-temporal hand gesture recognition (2002)

Several successful approaches to spatio-temporal signal processing such as speech recognition and hand gesture recognition have been proposed. Most of them involve time alignment which requires substantial computation and considerable memory storage. In this paper, we present a neural-network-based approach to spatio-temporal pattern recognition. This approach employs a powerful method based on hyperrectangular composite neural networks (HRCNNs) for selecting templates, therefore, considerable memory is alleviated. In addition, it greatly reduces substantial computation in the matching process because it obviates time alignment. Two databases consisted of 51 spatio-temporal hand gestures were utilized for verifying its performance. An encouraging experimental result confirmed the effectiveness of the proposed method.
Monitoring the formation of dynamic G-protein-coupled receptor–protein complexes in living cells (2005)

GPCRs (G-protein-coupled receptors) play an extremely important role in transducing extracellular signals across the cell membrane with high specificity and sensitivity. They are central to many of the body's endocrine and neurotransmitter pathways, and are consequently a major drug target. It is now clear that GPCRs interact with a range of proteins, including other GPCRs. Identifying and elucidating the function of such interactions will significantly enhance our understanding of cellular function, with the promise of new and improved pharmaceuticals. Biophysical techniques involving resonance energy transfer, namely FRET (fluorescence resonance energy transfer) and BRET (bioluminescence resonance energy transfer), now enable us to monitor the formation of dynamic GPCR-protein complexes in living cells, in real time. Their use has firmly established the concept of GPCR oligomerization, as well as demonstrating GPCR interactions with GPCR kinases, beta-arrestins, adenylate cyclase and a subunit of an inwardly rectifying K+ channel. The present review examines recent technological advances and experimental applications of FRET and BRET, discussing particularly how they have been adapted to extract an ever-increasing amount of information about the nature, specificity, stoichiometry, kinetics and agonist-dependency of GPCR-protein interactions.
Use of the parabiotic model in studies of cutaneous wound healing to define the participation of circulating cells (2010)

Previous experimental studies to assess the contribution of blood-borne circulating (BBC) cells to cutaneous wound healing have relied on discontinuous pulsing of labeled BBC elements or bone marrow transplant protocols. Such approaches do not allow the examination of stable BBC cells that have matured in a physiologically normal host. We have used a parabiotic murine model for cutaneous wound healing to evaluate the relative contribution of stable populations of peripheral blood cells expressing the green fluorescent protein (GFP) transgene in otherwise normal animals. Circulating cells (mature and immature) expressing the GFP transgene were easily detected and quantified in wounds of GFP- parabiotic twins during all evaluated stages of the healing response. Using multiple antibody probes, the relative contribution of various subsets of BBC cells could be comparatively assessed. In early wounds, some cells expressing mesenchymal epitopes were documented to be of hematopoietic origin, indicating the utility of this model in assessing cell plasticity in the context of tissue regeneration and repair. Application of this approach enables further investigation into the contribution of peripheral blood in normal and abnormal healing responses.
Characterization of a prostate carcinoma mucin‐like antigen (PMA) (1995)

A recently identified, high m.w. human tumor antigen, reactive with monoclonal antibody (MAb) PD41 and designated prostate mucin antigen (PMA), was found to have expression highly restricted to prostate carcinomas. Both biochemical and immunological characteristics of this antigen and its relationship to other tumor-associated mucins and various species of submaxillary mucins were evaluated. Immunohistochemical examination of submaxillary tissues revealed differential expression of this antigen and other human tumor-associated mucins, with MAb PD41 expression found only in bovine submaxillary gland serous cells. Neuraminidase treatment enhanced antibody binding by 50%, suggesting partial masking of the PD41 epitope by sialic acid. Antigenicity was reduced by treatment with alkaline-borohydride, sodium metaperiodate, beta-galactosidase, O-glycanase, and various proteolytic enzymes. MAb PD41 antibody binding also could be significantly reduced by selected lectins and sugars suggesting that the immunodominant carbohydrate involved in the epitope was an O-linked oligosaccharide containing N-acetyl galactosamine as a major component. This antigen was further distinguished from T, Tn, or Sialyl-Tn antigens and blood group carbohydrate antigens by radioimmunometric analyses. Cross-blocking and double determinant RIA experiments also showed a distinction between the PD41 epitope and several well-characterized tumor-associated mucin antigens such as MUCI, CEA, M344, OCI25, and AR3 as well as bovine submaxillary core protein. Our results indicate that the PD41-reactive epitope is a non-acidic O-linked carbohydrate or glycopeptide epitope with restricted expression in prostate carcinomas and bovine submaxillary glands. This expression is distinct from other mucin-like tumor-associated antigens identified in human prostate carcinomas.
Determining digital image origin using sensor imperfections (2005)

In this paper, we demonstrate that it is possible to use the sensor’s pattern noise for digital camera identification from images. The pattern noise is extracted from the images using a wavelet-based denoising filter. For each camera under investigation, we first determine its reference noise, which serves as a unique identification fingerprint. This could be done using the process of flat-fielding, if we have the camera in possession, or by averaging the noise obtained from multiple images, which is the option taken in this paper. To identify the camera from a given image, we consider the reference pattern noise as a high-frequency spread spectrum watermark, whose presence in the image is established using a correlation detector. Using this approach, we were able to identify the correct camera out of 9 cameras without a single misclassification for several hundred images. Furthermore, it is possible to perform reliable identification even from images that underwent subsequent JPEG compression and/or resizing. These claims are supported by experiments on 9 different cameras including two cameras of exactly same model (Olympus C765).
Early detection and monitoring of cancer in liquid biopsy: advances and challenges (2019)

KEYWORDS: Cancer monitoringcirculating tumor DNAearly detectionliquid biopsynext-generation sequencing
The microflora of the human ileum and intrabdominal colon: results of direct needle aspiration at surgery and evaluation of the technique. (1972)

Abstract The microflora of the terminal ileum, cecum, and transverse colon was defined in 10 patients undergoing elective cholecystectomy by direct needle aspiration of the bowel; the results were compared with the microbial counts in the feces. Facultative bacteria and obligate anaerobes were generally 2 to 4 logs more frequent in the feces than in the intra-abdominal colon. The microbial counts in the transverse colon and cecum were similar. The terminal ileum harbored a relatively sparse flora, contrasting with the large concentrations of microorganisms on the other side of the ileocecal valve. Studies in dogs revealed that the needle aspiration technique could obtain reproducible samples for bacteriologic study that differed by less than 1 log from direct intraluminal specimens. The addition of nonabsorbable markers to the diluting fluid used for aspirating bowel contents somewhat increased the accuracy of the method.
N-Methyl-d-Aspartate Antagonists in the Treatment of Parkinson's Disease (1991)

Current long-term treatment of Parkinson's disease is inadequate, and improved symptomatic and neuroprotective therapies are needed. Recent interest has focused on the use of antagonists of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptor in Parkinson's disease. Abnormally increased activity of the subthalamic nucleus is postulated to play a central pathophysiological role in the signs of Parkinson's disease, and NMDA antagonists may provide a means of decreasing this activity selectively. Like dopaminergic agonists, NMDA antagonists can reverse the akinesia and rigidity associated with monoamine depletion or neuroleptic-induced catalepsy. Very low doses of NMDA antagonists markedly potentiate the therapeutic effects of dopaminergic agonists. There is evidence that the beneficial effects of anticholinergic drugs and amantadine may be mediated, in part, by NMDA receptor blockade. Moreover, NMDA antagonists provide profound protection of dopaminergic neurons of the substantia nigra in the MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and methamphetamine models of Parkinson's disease. The clinical use of NMDA antagonists may prove useful in Parkinson's disease to treat symptoms and retard disease progression.
Ultrasound-Propelled Nanocups for Drug Delivery (2015)

Ultrasound-induced bubble activity (cavitation) has been recently shown to actively transport and improve the distribution of therapeutic agents in tumors. However, existing cavitation-promoting agents are micron-sized and cannot sustain cavitation activity over prolonged time periods because they are rapidly destroyed upon ultrasound exposure. A novel ultrasound-responsive single-cavity polymeric nanoparticle (nanocup) capable of trapping and stabilizing gas against dissolution in the bloodstream is reported. Upon ultrasound exposure at frequencies and intensities achievable with existing diagnostic and therapeutic systems, nanocups initiate and sustain readily detectable cavitation activity for at least four times longer than existing microbubble constructs in an in vivo tumor model. As a proof-of-concept of their ability to enhance the delivery of unmodified therapeutics, intravenously injected nanocups are also found to improve the distribution of a freely circulating IgG mouse antibody when the tumor is exposed to ultrasound. Quantification of the delivery distance and concentration of both the nanocups and coadministered model therapeutic in an in vitro flow phantom shows that the ultrasound-propelled nanocups travel further than the model therapeutic, which is itself delivered to hundreds of microns from the vessel wall. Thus nanocups offer considerable potential for enhanced drug delivery and treatment monitoring in oncological and other biomedical applications.
B Cells and Dendritic Cells from Vκ8 Light Chain Transgenic Mice Activate MRL- lpr/gld CD4+ T Cells (2006)

Autoreactive CD4+ T cells are required for full expression of disease in human systemic lupus erythematosus and in spontaneous murine lupus. However, the Ag specificity of these CD4+ T cells remains largely unknown. Rheumatoid factor (RF) B cells function as highly efficient APCs by taking up immune complexes (IC) and presenting IC constituents to T cells. We hypothesized that Ag-specific CD4+ T cells in lupus-prone mice could be identified by stimulating the CD4+ T cells with RF B cells from AM14 RF BCR transgenic mice pulsed with IC containing lupus-associated autoantibodies and autoantigens. This approach identified several independent T cell lines that proliferated robustly in response to IC-pulsed spleen cells from the AM14 RF BCR transgenic mice. However, these T cells did not recognize an IC constituent. Instead, these T cells recognized a determinant dependent on the inheritance of the transgene-encoded Vkappa8 L chain, most likely a neoantigen created by the insertion of the transgene into the genome. Additionally, although the precise nature of the neoantigen is not known, the T cells described in this report may provide a useful tool for examining the role of T cells in the RF autoantibody response.
Results of a Phase 2 NCI Multicenter Study of Romidepsin in Patients with Relapsed Peripheral T-Cell Lymphoma (PTCL). (2008)

Abstract Background: The histone deacetylase (HDAC) inhibitors are a class of epigenetic agents undergoing clinical testing. HDAC inhibitors modulate expression of genes involved in cell cycle regulation, leading to induction of differentiation or apoptosis. Romidepsin, a novel, HDAC inhibitor, has previously demonstrated activity as a single agent in patients (pts) with T-cell lymphoma in a phase 1 NCI study. Aims: To evaluate the efficacy and tolerability of romidepsin in the treatment of advanced PTCL and as an exploratory endpoint, to examine the molecular effects of romidepsin in both PTCL and cutaneous T-cell lymphoma (CTCL). Methods: This Phase 2, open-label, multi-arm, multicenter study enrolled 43 PTCL pts from the NCI and 9 extramural sites. CTCL pts were also enrolled in this study. The clinical results for pts with PTCL are presented here; the clinical results for the pts with CTCL and the biomarker analyses for both PTCL and CTCL are presented elsewhere. This study is now closed to accrual. Pts with relapsed or refractory PTCL or primary cutaneous anaplastic large cell lymphoma were eligible. Pts received romidepsin 14 mg/m2 as a 4-hr infusion on days 1, 8 and 15 every 28 days. Responses were assessed using elements of the IWG criteria and RECIST, as appropriate, for pts with lymph node involvement and extranodal disease. Results: 43 pts (23[53%] men and 20 [47%] women) with a mean age of 58.1 (range: 27 to 84) years were treated with romidepsin. 31 pts received ≥ 2 cycles of therapy. Mean number of prior therapies was 3.9 (range 1 to 12). Objective disease response rates (ORR) are summarized in the table. The mean number of cycles of treatment was 6.8 (range 1–37) and the overall median duration of response was 8.3 months (range 1.6 months to 4.8+ yrs) for all pts. The median duration of response for the 7 pts who achieved CR was 8.5 months (range 4.6 months to 4.8+yrs). The most frequent drug-related AEs (all grades, all cycles) were generally mild and included: nausea (86%; 0% ≥grade 3), fatigue (79%; 0% ≥grade 3), decreased platelets (70%; 7% ≥grade 3), and decreased AGC (63%; 5% ≥grade 3). One death, in a pt with significant cardiovascular disease who had achieved a CR, was considered possibly related to treatment. Conclusions: This study demonstrates tolerability and durable clinical benefit (ORR of 39% and median duration of response of 8.3 months) of romidepsin in pts with recurrent or refractory PTCL. Based on these promising results, a Phase 2B protocol investigating romidepsin in pts with progressive or relapsed PTCL is ongoing at multiple international centers. All Pts N=43 Pts ≥ 2 cycles N=31 ORR (CR+PR), n (%) 17 (39%) 17 (55%) PR, n (%) 10 (23%) 10 (32%) CR, n (%) 7 (16%) 7 (23%)
Review of the Relationships in the Montmorillonite Group of Clay Minerals (1958)

Abstract Since the publication of the paper on the minerals of the montmorillonite group by Ross and Hendricks (1945) some species have been added to the group, and minor changes in the acceptable species have become advisable. No radical changes are proposed, but a review of the members and their grouping will be presented. The acceptable dioctahedral members of the group appear to be montmorillonite, nontronite and volchonskoite. Beidellite should be discontinued. The acceptable trioetahedral members of the group appear to include saponite, hectorite, sauconite, stevensite and medmontite. Griffithite is probably a useful term for an iron-rich saponite. Representative chemical analyses and formulas have been assembled to illustrate relationships.
Selective killing of Paneth cells by intravenous administration of dithizone in rats. (1991)

Paneth cells are zinc-containing cells widely distributed in Lieberkühn's crypts of intestine in a variety of species. We found that rapid selective killing of Paneth cells took place after the intravenous (i.v.) injection of diphenylthiocarbazone (dithizone), a chelator forming a zinc dithizonate complex, in the rat. As soon as 5 min after the i.v. injection of dithizone, degeneration of Paneth cells occurred. At this stage, zinc dithizonate complexes were observed as purple-red granules in bright field microscopy. Thirty to 60 min later, Paneth cells were detached from the basement membrane and shed into the cryptic lumen. After 6 h, the cell debris in the crypts was no longer seen and the crypts once housing Paneth cells were now occupied by neighbouring crypt base columnar cells. Histochemically demonstrable zinc totally disappeared. After 12-24 h, however, definite Paneth cells began to resume. Histochemical staining for zinc was again positive at the apex of these cells. One week after dithizone administration, the number of Paneth cells increased twice as much as in uninjected control and histochemical staining for zinc was highly positive. After 2 weeks, Paneth cell hyperplasia subsided. X-ray microanalysis revealed that zinc was the most abundant metal in Paneth cells. We concluded that chelation of zinc and formation of zinc-dithizone complexes in Paneth cells' cytoplasm would be responsible for the selective degeneration observed after dithizone administration.