gebhart/patrepeval huggingface dataset:
Molecular cloning of human testicular angiotensin-converting enzyme: the testis isozyme is identical to the C-terminal half of endothelial angiotensin-converting enzyme. (1989)

Angiotensin-converting enzyme (ACE; EC 3.4.15.1) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. Testis contains a unique, androgen-dependent ACE isozyme of unknown function. We have determined the cDNA sequence for human testicular ACE; it encodes a protein that is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence [Soubrier, F., Alhenc-Gelas, F., Hubert, C., Allegrini, J., John, M., Tregear, G. & Corvol, P. (1988) Proc. Natl. Acad. Sci. USA 85, 9386-9390]. The full-length human testis ACE cDNA was constructed from a composite of cloned cDNAs, obtained by a combination of (i) immunoscreening and hybridization screening of a human testicular cDNA library in lambda gt11 and (ii) hybridization screening of human testis cDNAs constructed with ACE-specific primers and amplified by the polymerase chain reaction. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. The testis enzyme contains the second of the two putative metal-binding sites (His-Glu-Met-Gly-His) identified in endothelial ACE. This indicates that the functionally active catalytic site is within the C-terminal domain of the endothelial enzyme, accounting for the previous finding that these two structurally dissimilar isozymes are virtually identical catalytically. Of 22 testis ACE cDNAs cloned and sequenced, 3 have unique 5' regions, consisting of inserted, deleted, or substituted sequences up to 328 base pairs long, which have apparently arisen by alternative pre-mRNA splicing.
Thermochemical CO2 and CO2/H2O Splitting over NiFe2O4 for Solar Fuels Synthesis (2014)

Within the framework of the recent trend to identify efficient ways of producing solar syngas (CO/H2), the two-step redox based solar thermochemical water dissociation cycle, already employed at a semi-pilot scale for the renewable production of H2, can be modified to include CO2 and/or combined CO2/H2O splitting. The present work relates to Ni-ferrite, as candidate redox material to be employed in Concentrated Solar Power (CSP)-aided thermochemical processes for CO2 and CO2/H2O splitting for the renewable production of CO and syngas respectively. The mixed oxide was synthesized via the Self-propagating High temperature Synthesis (SHS) method and subsequently calcined under air at 1400oC for 1 h. Upon calcination, the material obtained the single phase spinel structure. The material was tested, in the form of powder and as a small cylinder-shaped porous structured body, in a lab-scale fixed bed reactor. The experimental protocol involved a thermal activation step of the material for 1 h at 1400oC under N2 flow, the CO2 or CO2/H2O splitting step at 1100oC for 30 min, which resulted in the production of CO or CO/H2 respectively and the thermal reduction step under N2 at 1400oC for 30 min. The effect of CO2 concentration in the feed gas (4%-100%) was investigated in two-cycle experimental runs. In addition, a preliminary durability test was conducted under pure CO2 flow for 8 consecutive splitting and thermal reduction steps. Co-feeding of H2O and CO2 was also conducted for two different compositions; 8%H2O/4% CO2/N2 and 16%H2O/8% CO2/N2). The porous structured body showed somewhat lower yield, in terms of CO2 splitting, compared to the powder.
A VERSATILE SOFTWARE ARCHITECTURE FOR VIRTUAL AUDIO SIMULATIONS (2001)

Existing real-time audio rendering architectures provide rigid development frameworks which are not adapted to a wide range of applications. In particular, experimenting with new rendering techniques is virtually impossible. In this paper,
Comparative effects of six probiotic strains on immune functionin vitro (2011)

There is considerable interest in the strain specificity of immune modulation by probiotics. The present study compared the immunomodulatory properties of six probiotic strains of different species and two genera in a human peripheral blood mononuclear cell (PBMC) model in vitro. Live cells of lactobacilli (Lactobacillus casei Shirota, L. rhamnosus GG, L. plantarum NCIMB 8826 and L. reuteri NCIMB 11951) and bifidobacteria (Bifidobacterium longum SP 07/3 and B. bifidum MF 20/5) were individually incubated with PBMC from seven healthy subjects for 24 h. Probiotic strains increased the proportion of CD69+ on lymphocytes, T cells, T cell subsets and natural killer (NK) cells, and increased the proportion of CD25+, mainly on lymphocytes and NK cells. The effects on activation marker expression did not appear to be strain specific. NK cell activity was significantly increased by all six strains, without any significant difference between strains. Probiotic strains increased production of IL-1β, IL-6, IL-10, TNF-α, granulocyte-macrophage colony-stimulating factor and macrophage inflammatory protein 1α to different extents, but had no effect on the production of IL-2, IL-4, IL-5 or TNF-β. The cytokines that showed strain-specific modulation included IL-10, interferon-γ, TNF-α, IL-12p70, IL-6 and monocyte chemotactic protein-1. The Lactobacillus strains tended to promote T helper 1 cytokines, whereas bifidobacterial strains tended to produce a more anti-inflammatory profile. The results suggest that there was limited evidence of strain-specific effects of probiotics with respect to T cell and NK cell activation or NK cell activity, whereas production of some cytokines was differentially influenced by probiotic strains.
Noninvasive fetal blood group genotyping of rhesus D, c, E and of K in alloimmunised pregnant women: evaluation of a 7-year clinical experience (2011)

Noninvasive fetal blood group genotyping is accurate and applicable in a clinical diagnostic setting.
Chromatographic Alignment of ESI-LC-MS Proteomics Data Sets by Ordered Bijective Interpolated Warping (2006)

Mass spectrometry proteomics typically relies upon analyzing outcomes of single analyses; however, comparing raw data across multiple experiments should enhance both peptide/protein identification and quantitation. In the absence of convincing tandem MS identifications, comparing peptide quantities between experiments (or fractions) requires the chromatographic alignment of MS signals. An extension of dynamic time warping (DTW), termed ordered bijective interpolated warping (OBI-Warp), is presented and used to align a variety of electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS) proteomics data sets. An algorithm to produce a bijective (one-to-one) function from DTW output is coupled with piecewise cubic hermite interpolation to produce a smooth warping function. Data sets were chosen to represent a broad selection of ESI-LC-MS alignment cases. High confidence, overlapping tandem mass spectra are used as standards to optimize and compare alignment parameters. We determine that Pearson's correlation coefficient as a measure of spectra similarity outperforms covariance, dot product, and Euclidean distance in its ability to produce correct alignments with optimal and suboptimal alignment parameters. We demonstrate the importance of penalizing gaps for best alignments. Using optimized parameters, we show that OBI-Warp produces alignments consistent with time standards across these data sets. The source and executables are released under MIT style license at http://obi-warp.sourceforge.net/.
5-(Trifluoromethyl)bacteriorhodopsin does not translocate protons (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT5-(Trifluoromethyl)bacteriorhodopsin does not translocate protonsV. Jayathirtha. Rao, Fadila. Derguini, Koji. Nakanishi, Takeo. Taguchi, Akihiko. Hosada, Yuji. Hanzawa, Toshiro. Kobayashi, Chandra. Pande, and Robert H. CallenderCite this: J. Am. Chem. Soc. 1986, 108, 19, 6077–6078Publication Date (Print):September 1, 1986Publication History Published online1 May 2002Published inissue 1 September 1986https://pubs.acs.org/doi/10.1021/ja00279a087https://doi.org/10.1021/ja00279a087research-articleACS PublicationsRequest reuse permissionsArticle Views60Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Activity of Pleconaril against Enteroviruses (1999)

The activity of pleconaril in cell culture against prototypic enterovirus strains and 215 clinical isolates of the most commonly isolated enterovirus serotypes was examined. The latter viruses were isolated by the Centers for Disease Control and Prevention during the 1970s and 1980s from clinically ill subjects. Pleconaril at a concentration of </=0.03 microM inhibited the replication of 50% of all clinical isolates tested. Ninety percent of the isolates were inhibited at a drug concentration of </=0.18 microM. The most sensitive serotype, echovirus serotype 11, was also the most prevalent enterovirus in the United States from 1970 to 1983. Pleconaril was further tested for oral activity in three animal models of lethal enterovirus infection: coxsackievirus serotype A9 infection in suckling mice, coxsackievirus serotype A21 strain Kenny infection in weanling mice, and coxsackievirus serotype B3 strain M infection in adult mice. Treatment with pleconaril increased the survival rate in all three models for both prophylactic and therapeutic dosing regimens. Moreover, pleconaril dramatically reduced virus levels in target tissues of coxsackievirus serotype B3 strain M-infected animals. Pleconaril represents a promising new drug candidate for potential use in the treatment of human enteroviral infections.
Epidermal Cell Fate Determination in Arabidopsis : Patterns Defined by a Steroid-Inducible Regulator (1994)

The Arabidopsis mutant ttg lacks both trichomes (epidermal hairs) and anthocyanin pigments. Trichomes and anthocyanins are restored by the constitutive expression of the maize transcriptional regulator (R). The expression of an R-glucocorticoid receptor chimeric protein results in a steroid hormone-dependent, conditional allele of R that functions in whole Arabidopsis plants. The response of the chimeric protein to pulses of hormone was used to define the pattern and timing of trichome formation on the developing leaf epidermis. Each adaxial epidermal leaf cell appears to have an equal probability of differentiating into a trichome; there is a temporal zone of decision for trichome cell fate that proceeds as a wave from the tip to the base of developing leaves.
Studies on In Vivo Formation of Nitroso Compounds (III) (1975)

The concentrations of nitrite and nitrate in human saliva directly collected from salivary ducts were studied.In the previous papers of this series, it was observed that human saliva contained nitrite and nitrate and their concentrations were affected by the amount of nitrate in ingested foods.In the present paper, it was elucidated that high amounts of nitrite and nitrate were detected in saliva which was accumulated in mouth, but only nitrate was detected in saliva which was directly collected from the ducts of salivary glands. The similar phenomena were observed during 10hr after the ingestion of sodium nitrate.From these results, it seemed that nitrite was not secreted in saliva, but produced newly from secreted nitrate in mouth.
A modular degron library for synthetic circuits in mammalian cells (2019)

Tight control over protein degradation is a fundamental requirement for cells to respond rapidly to various stimuli and adapt to a fluctuating environment. Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons) for the precise regulation of protein expression profiles in mammalian cells by modulating target protein half-lives in a predictable manner. Using the well-established tetracycline gene-regulation system as a model, we show that the dynamics of protein expression can be tuned by fusing appropriate degron tags to gene regulators. Next, we apply this degron library to tune a synthetic pulse-generating circuit in mammalian cells. With this toolbox we establish a set of pulse generators with tailored pulse lengths and magnitudes of protein expression. This methodology will prove useful in the functional roles of essential proteins, fine-tuning of gene-expression systems, and enabling a higher complexity in the design of synthetic biological systems in mammalian cells.
Transdermal fentanyl during high-dose chemotherapy and autologous stem cell support. (2000)

We report on our experience in the use of transdermal fentanyl in management of acute pain due to mucositis WHO-grade IV during high-dose chemotherapy (HDC) and autologous stem cell support (APBSCT). Between 8/96 and 12/98 74 patients received HDC and PBSCT for progressive disease or relapse of non-Hodgkin's lymphoma (n=32), multiple myeloma (n=37), Hodgkin's lymphoma (n=5). All patients suffered from mucositis WHO-grade IV with a need for continuous pain management. Instead of pethidine i.v. fentanyl TTS was used. Sufficient analgesia was achieved mostly with a dose of 50 microg/h. There was no need of supplementary analgesia. Relevant fentanyl-associated side effects were not seen. Patient compliance and acceptance were excellent. The results suggest that transdermal fentanyl is reliable in pain management of chemotherapy-associated mucositis grade IV.
Bioelectricity and regeneration: large currents leave the stumps of regenerating newt limbs. (1977)

Electrical currents near regenerating newt limbs were measured with a recently developed vibrating probe. Steady currents with local surface densities of 10 to 100 muA/cm2 or more leave the end of the stump during the first 5-10 days after amputation and are balanced by currents with densities of only 1-3 muA/cm2 that enter the intact skin around the stump. They are immediately dependent upon the entry of sodium ions into this skin and are therefore inferred to be skin-driven. The outward currents are comparable in direction, density, duration, and position to artificially imposed currents previously found sufficient to induce significant regeneration of amputated adult frog limbs. This comparison suggests that the endogenous stump currents play some causal role in initiating regeneration.
On the $Z_p$-extensions over $Q(sqrt{m})$ (1998)

Let $k = Q(\sqrt{m})$ be a real quadratic field. In this paper, the following theorems on p-divisibility of the class number h of k are studied for each prime pp. Theorem 1. If the discriminant of k has at least three distinct prime divisors, then 2 divides h. Theorem 2. If an odd prime p divides h, then p divides $B_{a,\chi\omega^{-1}}$, where $\chi$ is the nontrivial character of k, and $\omega$ is the Teichmuller character for pp. Theorem 3. Let $h_n$ be the class number of $k_n$, the nth layer of the $Z_p$-extension $k_\infty$ of k. If p does not divide $B_{a,\chi\omega^{-1}}$, then $p \notmid h_n$ for all $n \geq 0$.
Characterization of rhBMP-2 pharmacokinetics implanted with biomaterial carriers in the rat ectopic model (1999)

Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a member of the bone morphogenetic protein family involved in de novo bone induction. Successful use of rhBMP-2 requires implantation with a biomaterial which can act as a scaffold for cell invasion for osteoinduction and retains rhBMP-2 at a site of implantation. This study was carried out to characterize rhBMP-2 pharmacokinetics from a variety of biomaterial carriers in a rat ectopic model. Retention of rhBMP-2 within carriers after 3 h was variable among the carriers (range, 75-10%), with collagenous sponges retaining the highest fraction of implanted dose. A gradual loss of rhBMP-2 was subsequently observed, the kinetics of which was strongly dependent on the implanted carrier. Collagenous carriers were observed to lose rhBMP-2 gradually from the implant site, whereas some of the mineral-based carriers retained a fraction of implanted rhBMP-2 within the implants. These differences in protein pharmacokinetics among carriers, in addition to their physicochemical nature, are expected to affect the biological activity of implanted rhBMP-2.
Irreversible inactivation of snake venom l‐amino acid oxidase by covalent modification during catalysis of l‐propargylglycine (2013)

Snake venom l-amino acid oxidase (SV-LAAO, a flavor-enzyme) has attracted considerable attention due to its multifunctional nature, which is manifest in diverse clinical and biological effects such as inhibition of platelet aggregation, induction of cell apoptosis and cytotoxicity against various cells. The majority of these effects are mediated by H2O2 generated during the catalytic conversion of l-amino acids. The substrate analog l-propargylglycine (LPG) irreversibly inhibited the enzyme from Crotalus adamanteus and Crotalus atrox in a dose- and time-dependent manner. Inactivation was irreversible which was significantly protected by the substrate l-phenylalanine. A Kitz-Wilson replot of the inhibition kinetics suggested formation of reversible enzyme-LPG complex, which occurred prior to modification and inactivation of the enzyme. UV-visible and fluorescence spectra of the enzyme and the cofactor strongly suggested formation of covalent adduct between LPG and an active site residue of the enzyme. A molecular modeling study revealed that the FAD-binding, substrate-binding and the helical domains are conserved in SV-LAAOs and both His223 and Arg322 are the important active site residues that are likely to get modified by LPG. Chymotrypsin digest of the LPG inactivated enzyme followed by RP-HPLC and MALDI mass analysis identified His223 as the site of modification. The findings reported here contribute towards complete inactivation of SV-LAAO as a part of snake envenomation management.
The Hyperintense Acute Reperfusion Marker on Fluid-Attenuated Inversion Recovery Magnetic Resonance Imaging Is Caused by Gadolinium in the Cerebrospinal Fluid (2011)

Our study yields first direct evidence in humans that the imaging phenomenon HARM is indeed caused by leakage of gadolinium-based contrast agents into the cerebrospinal fluid.
The Conversion of Testosterone to 5α-Androstan-17β-ol-3-one by Rat Prostate in Vivo and in Vitro (1968)

The identity of the nonpolar transformation products of testosterone-1,2-3H, the time course of the appearance of these metabolites in various tissue compartments, and the subcellular distribution of testosterone and its metabolites in prostate have been investigated following the intravenous administration of the hormone to normal and to functionally hepatectomized rats. Evidence has been obtained in both types of animals that within 1 min following its administration testosterone is taken up by the prostate, and at least 90% is converted to three products, androstandiol, dihydrotestosterone, and androsterone. From prostatic nuclei, however, only testosterone-3H and dihydrotestosterone-3H were recovered for as long as 2 hours following testosterone injection. Furthermore, it has been shown that in the presence of a NADPH2-generating system prostatic nuclei convert testosterone to dihydrotestosterone, whereas prostatic cytoplasm, in addition, reduces dihydrotestosterone to androstandiol. The nuclear enzyme which performs this reaction has been partially characterized and appears to be located within the chromatin. Finally, the tissue distribution of dihydrotestosterone-3H has been investigated at a short time interval following testosterone-1,2-3H injection; this metabolite was detected only in prostate, seminal vesicle, preputial gland, kidney, and plasma.
Constitutively Activated ALK2 and Increased SMAD1/5 Cooperatively Induce Bone Morphogenetic Protein Signaling in Fibrodysplasia Ossificans Progressiva (2008)

Fibrodysplasia ossificans progressiva (FOP) is a rare autosomal dominant disorder characterized by congenital malformation of the great toes and by progressive heterotopic bone formation in muscle tissue. Recently, a mutation involving a single amino acid substitution in a bone morphogenetic protein (BMP) type I receptor, ALK2, was identified in patients with FOP. We report here that the identical mutation, R206H, was observed in 19 Japanese patients with sporadic FOP. This mutant receptor, ALK2(R206H), activates BMP signaling without ligand binding. Moreover, expression of Smad1 and Smad5 was up-regulated in response to muscular injury. ALK2(R206H) with Smad1 or Smad5 induced osteoblastic differentiation that could be inhibited by Smad7 or dorsomorphin. Taken together, these findings suggest that the heterotopic bone formation in FOP may be induced by a constitutively activated BMP receptor signaling through Smad1 or Smad5. Gene transfer of Smad7 or inhibition of type I receptors with dorsomorphin may represent strategies for blocking the activity induced by ALK2(R206H) in FOP.
Synchronization of oscillators coupled through narrow-band networks (2001)

The ability of two coupled oscillators to synchronize depends critically on the coupling network. Previous analyses have accurately predicted the performance of quasi-optical microwave oscillator arrays for both weak and strong coupling, but have been limited to coupling networks with bandwidths considerably larger than the locking bandwidths of the oscillators. In this paper, the authors develop a method for deriving a suitable system of nonlinear differential equations describing the oscillator amplitude and phase dynamics using a generalization of Kurokawa's method. The method is applied to the case of two Van der Pol oscillators coupled through a resonant network for a wide range of coupling strengths and bandwidths. Simple approximate formulas are developed for the size of the frequency locking region as functions of the basic circuit parameters.
Selective Head Cooling in Newborn Infants After Perinatal Asphyxia: A Safety Study (1998)

Mild selective head cooling combined with mild systemic hypothermia in term newborn infants after perinatal asphyxia is a safe and convenient method of quickly reducing cerebral temperature with an increased gradient between the surface of the scalp and core temperature. The safety of mild hypothermia with selective head cooling is in contrast with the historical evidence of adverse effects with greater depths of whole-body hypothermia. This safety study and the strong experimental evidence for improved cerebral outcome justify a multicenter trial of selective head cooling for neonatal encephalopathy in term infants.