gebhart/patrepeval huggingface dataset:
Differential Reactivity of a Novel Monoclonal Antibody (DF3) with Human Malignant versus Benign Breast Tumors (1984)

We have defined a human breast tumor associated antigen using a murine monoclonal antibody (MAb DF3) prepared against a membrane-enriched fraction of a human breast carcinoma. This antigen has a MW of 290 kD and is detectable on the cell surface of human breast carcinoma cells using a live cell radioimmunoassay and fluorescence flow cytometry. More important, immunoperoxidase staining with MAb DF3 clearly distinguishes malignant and benign breast lesions. A cytoplasmic staining pattern has been observed with 40 of 51 (78%) breast carcinomas, but only one of 13 fibroadenoma or fibrocystic disease specimens. In contrast, reactivity of benign breast lesions with MAb DF3 primarily occurs along apical borders on ductules. These results demonstrate that the DF3 antigen is present on apical borders of more differentiated secretory mammary epithelial cells and in the cytosol of less differentiated cells.
RF pulse optimization for Bloch‐Siegert B mapping (2011)

The Bloch-Siegert (B-S) method of B ₁⁺ mapping has been shown to be fast and accurate, yet has high SAR and moderately long TE. These limitations can lengthen scan times and incur signal loss due to B(0) inhomogeneity, particularly at high field. The B-S method relies on applying a band-limited off-resonant B-S radiofrequency pulse to induce a B ₁⁺-dependent frequency-shift for resonant spins. A method for optimizing the B-S radiofrequency pulse is presented here, which maximizes B-S B ₁⁺ measurement sensitivity for a given SAR and T(2) . A 4-ms optimized pulse is shown to have 35% less SAR compared with the conventional 6-ms Fermi pulse while still improving B ₁⁺ map angle-to-noise ratio by 22%. The optimized pulse performance is validated both in phantom and in vivo brain imaging at 7 T.
Defined TLR3-specific adjuvant that induces NK and CTL activation without significant cytokine production in vivo (2015)

Ligand stimulation of the Toll-like receptors (TLRs) triggers innate immune response, cytokine production and cellular immune activation in dendritic cells. However, most TLR ligands are microbial constituents, which cause inflammation and toxicity. Toxic response could be reduced for secure immunotherapy through the use of chemically synthesized ligands with defined functions. Here we create an RNA ligand for TLR3 with no ability to activate the RIG-I/MDA5 pathway. This TLR3 ligand is a chimeric molecule consisting of phosphorothioate ODN-guided dsRNA (sODN-dsRNA), which elicits far less cytokine production than poly(I:C) in vitro and in vivo. The activation of TLR3/TICAM-1 pathway by sODN-dsRNA effectively induces natural killer and cytotoxic T cells in tumour-loaded mice, thereby establishing antitumour immunity. Systemic cytokinemia does not occur following subcutaneous or even intraperitoneal administration of sODN-dsRNA, indicating that TICAM-1 signalling with minute local cytokines sufficiently activate dendritic cells to prime tumoricidal effectors in vivo.
Production of Ovarian Tumors by Hormone Administration. (1981)

The influence of various extrinsic hormones was observed by using the clipping method to imbed and fix chemical carcinogens in the ovarian parenchyma. Among the hormones used, ACH and estrogen appeared to accelerate tumor growth. Although the incidence of solid tumors showed little difference among the test groups by the 20 th week, the incidence increased to 90% by the 40 th week in the ACH group. In hormone-given groups, incidence of tumors was low until the 20 th week, particularly in the androgen and progesteron groups. After that point the incidence of tumors incerased more in the hormone-given groups than in the control group. The estrogen group had the highest total incidence of cystic tumor, when compared with the control. The process of generating adenocarcinoma and sarcoma was fundamentally similar in the treated groups and the control. However, the time of appearance of tumors is the hormone-traced groups, except in the ACH group, differed from the control, particularly in the estrogen group.
Functionalized Mesoporous Silica Nanoparticle-Based Drug Delivery System to Rescue Acrolein-Mediated Cell Death (2008)

MSNs modified with drug/polymer constructs provide significant neuroprotection to cells damaged by a usually lethal exposure to acrolein.
Induction of Lens Differentiation by Activation of a bZIP Transcription Factor, L-Maf (1998)

After the vertebrate lens is induced from head ectoderm, lens-specific genes are expressed. Transcriptional regulation of the lens-specific alphaA-crystallin gene is controlled by an enhancer element, alphaCE2. A gene encoding an alphaCE2-binding protein, L-maf(lens-specific maf), was isolated. L-maf expression is initiated in the lens placode and is restricted to lens cells. The gene product L-Maf regulates the expression of multiple genes expressed in the lens, and ectopic expression of this transcription factor converts chick embryonic ectodermal cells and cultured cells into lens fibers. Thus, vertebrate lens induction and differentiation can be triggered by the activation of L-Maf.
A small, synthetic peptide for gene delivery via the serpin-enzyme complex receptor (2001)

A small (33 amino acid), bifunctional, synthetic peptide represents a highly efficient and readily standardised DNA vector for the SECR. The effectiveness of this peptide depends on the distance of the K16 moiety from the targeting ligand. High salt concentrations are not required to form effective vector/DNA complexes.
Orthogonal frequency‐division multiple access and its application to CATV networks (1998)

Abstract We introduce the concept of orthogonal frequency‐division multiple access (OFDMA) and investigate its application to send upstream information from subscriber premises to the cable head‐end in cable TV (CATV) networks. The carrier and timing synchronizations needed to ensure orthogonality between different subscriber signals are achieved by sending common pilot signals from the head‐end. Whereas N demodulators are required to accommodate N simultaneous users in conventional FDMA systems, OFDMA only requires a single demodulator followed by an N ‐point discrete Fourier transform (DFT). We analyze the performance of this technique and compare it to time‐division multiple access (TDMA) which appears in several international standards. Our results indicate that OFDMA is an attractive multiple access technique which offers increased robustness to narrowband interference, impulse noise, and other signal impairments which characterize typical CATV networks.
p53: prospects for cancer gene therapy. (1998)

Initially described as an oncogene, at the end of the 1980s, the wild-type p53 gene (TP53) was later shown to be capable of suppressing the proliferation of transformed cells. In the following years, an increasing number of studies demonstrated that intact p53 function is essential for the maintenance of the non-tumorigenic phenotype of cells. Indeed, functional inactivation of the p53 protein is one of the most common alterations observed in human cancers. More recently, it has been shown that inactivation of the TP53 tumor suppressor gene may lead to radioresistant and chemoresistant tumors, possibly by the induction of apoptosis-resistant phenotypes. Finally, a large number of in vitro and in vivo transduction experiments have demonstrated that exogenous TP53 overexpression can suppress the transformed phenotype of many cell types by inducing growth arrest, apoptosis or cell differentiation. All of these findings have rendered p53 a potentially helpful target for the therapy of many types of human cancers. In this review we shall discuss the different approaches to p53-related cancer therapy that have been proposed on the basis of this large number of experimental studies, and we shall try to dissect the biological questions that are still open and need to be clarified to improve p53-based therapy.
Measurements of nitric oxide on the heme iron and β-93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation (2003)

Nitric oxide has been proposed to be transported by hemoglobin as a third respiratory gas and to elicit vasodilation by an oxygen-linked (allosteric) mechanism. For hemoglobin to transport nitric oxide bioactivity it must capture nitric oxide as iron nitrosyl hemoglobin rather than destroy it by dioxygenation. Once bound to the heme iron, nitric oxide has been reported to migrate reversibly from the heme group of hemoglobin to the beta-93 cysteinyl residue, in response to an oxygen saturation-dependent conformational change, to form an S-nitrosothiol. However, such a transfer requires redox chemistry with oxidation of the nitric oxide or beta-93 cysteinyl residue. In this article, we examine the ability of nitric oxide to undergo this intramolecular transfer by cycling human hemoglobin between oxygenated and deoxygenated states. Under various conditions, we found no evidence for intramolecular transfer of nitric oxide from either cysteine to heme or heme to cysteine. In addition, we observed that contaminating nitrite can lead to formation of iron nitrosyl hemoglobin in deoxygenated hemoglobin preparations and a radical in oxygenated hemoglobin preparations. Using 15N-labeled nitrite, we clearly demonstrate that nitrite chemistry could explain previously reported results that suggested apparent nitric oxide cycling from heme to thiol. Consistent with our results from these experiments conducted in vitro, we found no arterial/venous gradient of iron nitrosyl hemoglobin detectable by electron paramagnetic resonance spectroscopy. Our results do not support a role for allosterically controlled intramolecular transfer of nitric oxide in hemoglobin as a function of oxygen saturation.
Automatic parsing of news video (1994)

Video content parsing is possible when one has an a priori model of a video's structure based on domain knowledge. This paper presents work on using domain knowledge to parse content of news video programs. Approaches to locating and identifying frame structure models based on temporal and spatial structure of news video data, along with algorithms to apply these models in parsing news video, have been developed and are presented in detail in this paper. Experimental results are also discussed in detail to evaluate the approaches and algorithms. Finally, proposals for future work are summarized.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Selective translocation of the A chain of diphtheria toxin across the membrane of purified endosomes. (1992)

Translocation is a necessary and rate-limiting step for diphtheria toxin (DT) cytotoxicity. We have reconstituted DT translocation in a cell-free system using endosomes purified from lymphocytes and have demonstrated this using two different probe/cell systems, which provided identical results: 125I-DT/human CEM cells and 125I-transferrin-DT/mouse BW cells. The cell-free DT translocation process was found to be dependent on the presence of the pH gradient endosome (pH 5.3)/cytosol (pH 7). Among the pH equilibrating agents, nigericin (5 microM) was found to be the most effective, inhibiting DT translocation by 88%. An optimum pH value of 7 on the cytosolic side of the membrane (pH gradient approximately 1.7) was determined. ATP per se is not required for DT translocation. 125I-DT translocation was 3-fold more active from late than from early endosomes, probably because of their slightly more acidic pH. Only the A chain of the toxin was found to escape from either 125I-DT/CEM or 125I-transferrin-DT/BW endosomes. Translocation of control endosome labels (125I-transferrin and 125I-horseradish peroxidase) was never observed. We also show that DT receptors present on resistant (mouse) cells block the translocation of the toxin and are responsible for the resistance of these cells to DT.
Human testicular protein TPX1/CRISP-2: localization in spermatozoa, fate after capacitation and relevance for gamete interaction (2005)

Testicular protein Tpx-1, also known as CRISP-2, is a cysteine-rich secretory protein specifically expressed in the male reproductive tract. Since the information available on the human protein is limited to the identification and expression of its gene, in this work we have studied the presence and localization of human Tpx-1 (TPX1) in sperm, its fate after capacitation and acrosome reaction (AR), and its possible involvement in gamete interaction. Indirect immunofluorescence studies revealed the absence of significant staining in live or fixed non-permeabilized sperm, in contrast to a clear labelling in the acrosomal region of permeabilized sperm. These results, together with complementary evidence from protein extraction procedures strongly support that TPX1 would be mainly an intra-acrosomal protein in fresh sperm. After in vitro capacitation and ionophore-induced AR, TPX1 remained associated with the equatorial segment of the acrosome. The lack of differences in the electrophoretic mobility of TPX1 before and after capacitation and AR indicates that the protein would not undergo proteolytical modifications during these processes. The possible involvement of TPX1 in gamete interaction was evaluated by the hamster oocyte penetration test. The presence of anti-TPX1 during gamete co-incubation produced a significant and dose-dependent inhibition in the percentage of penetrated zona-free hamster oocytes without affecting sperm motility, the AR or sperm binding to the oolema. Together, these results indicate that human TPX1 would be a component of the sperm acrosome that remains associated with sperm after capacitation and AR, and is relevant for sperm-oocyte interaction.
S-nitroso human serum albumin reduces ischaemia/reperfusion injury in the pig heart after unprotected warm ischaemia (2007)

Long-lasting release of NO by S-NO-HSA prevented uncoupling of eNOS and thereby improved systolic and diastolic function, myocardial perfusion, and the energetic reserve of the heart after I/R injury.
N-Phenyl-2-indolinones and N-phenylindolines. New class of antidepressant agents (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTN-Phenyl-2-indolinones and N-phenylindolines. New class of antidepressant agentsA. Canas-Rodriguez and P. R. LeemingCite this: J. Med. Chem. 1972, 15, 7, 762–770Publication Date (Print):July 1, 1972Publication History Published online1 May 2002Published inissue 1 July 1972https://doi.org/10.1021/jm00277a017RIGHTS & PERMISSIONSArticle Views328Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1005 KB) Get e-Alerts Get e-Alerts
CAMPATH-1H in multiple sclerosis (1996)

In a pilot study, seven patients with multiple sclerosis were treated with CAMPATH-IH which targets the CD52 antigen present on lymphocytes and monocytes. There was a substantial reduction in disease activity as measured by gadoliunium-enhancing lesions on MRI. Encouraged by this result a further seven patients have been treated with CAMPATH-IH; four also received anti-CD4 antibody. Lymphopaenia developed rapidly and was sustained for at least one year. In 12 patients, the first infusion of antibody was characterised by significant exacerbation or re-awakening of pre-existing symptoms lasting several hours. These clinical effects of antibody treatment correlated with increased levels of circulating cytokines. Peak levels of tumour necrosis factor alpha (TNF alpha) and interferon gamma (IFN gamma) occurred at 2 h whereas the rise in interleukin-6 (IL-6) was significantly delayed and peaked at 4 h after starting antibody treatment. The neurological symptoms could not be attributed directly to pyrexia and were not provoked (in one patient) by an artificial rise in temperature. In the remaining two patients, a single pre-treatment with intravenous methylprednisolone (500 mg) prevented both the transient increase in neurological symptoms and the cytokine release. Our results suggest that soluble immune mediators contribute to symptom production in multiple sclerosis by directly or indirectly blocking conduction through partially demyelinated pathways.
Proliferation Potential-Related Protein, an Ideal Esophageal Cancer Antigen for Immunotherapy, Identified Using Complementary DNA Microarray Analysis (2004)

The expression of PP-RP in esophageal cancer cells was significantly higher than in normal cells, and the CTLs recognizing PP-RP killed tumor cells in vitro and also showed tumor rejection effects in a xenograft model. Therefore, PP-RP may prove to be an ideal tumor antigen useful for diagnosis and immunotherapy for patients with esophageal cancer. cDNA microarray analysis is a useful method to identify ideal tumor-associated antigens.
A highlight scene detection and video summarization system using audio feature for a personal video recorder (2005)

The personal video recorder such as recordable-DVD recorder, Blu-ray disc recorder and/or hard disc recorder has become popular for a large volume storage device for video/audio content data and a browsing function that would quickly provide a desired scene to the user is required as an essential part of such a large capacity recording/playback system. We propose a highlight scene detection function by using only 'audio' features and realize a browsing function for the recorder that enables completely automatic detection of sports highlights. We detect sports highlights by identifying portions with "commentator's excited speech" using Gaussian mixture models (GMM's) trained using the MDL criterion. Our computation is carried out directly on the MDCT coefficients from the AC-3 coefficients thus giving us a tremendous speed advantage. Our accuracy of detection of sports highlights is high across a variety of sports.
Convoluted array elements and reduced size unit cells for frequency-selective surfaces (1991)

When the curvature of a surface that is to be made frequency selective is not small, it modifies the array lattice and element geometry, altering the transmission performance of the surface. For a given degree of curvature, small unit cells are distorted less. The transmission response of a typical array of conducting elements has a main reflection resonance at a wavelength λ1, with further nulls at shorter wavelengths. We have attempted to reduce the unit cell dimension p as much as possible for a given reflection wavelength λ1, thereby increasing its separation from grating responses. The ratio λ1/p has been used as a figure of merit. An array of linear dipoles on a square lattice of side p is a standard of comparison, a typical case giving a figure of merit of 1.6. A Jerusalem cross array gives a value of about 4.5. By convoluting the conductors of a square loop element, or by using, as the array elements, the low order generations of space filling curves, such as that described by Hilbert, we have obtained values of λ1/p in the range 7–12. The unit cell areas are lower than that of the dipole standard by factors averaging about 40.
Antagonism of Notch signaling activity by members of a novel protein family encoded by the Bearded and Enhancer of split gene complexes (2000)

Cell-cell signaling through the Notch receptor is a principal mechanism underlying cell fate specification in a variety of developmental processes in metazoans, such as neurogenesis. In this report we describe our investigation of seven members of a novel gene family in Drosophila with important connections to Notch signaling. These genes all encode small proteins containing predicted basic amphipathic (&agr;)-helical domains in their amino-terminal regions, as described originally for Bearded; accordingly, we refer to them as Bearded family genes. Five members of the Bearded family are located in a newly discovered gene complex, the Bearded Complex; two others reside in the previously identified Enhancer of split Complex. All members of this family contain, in their proximal upstream regions, at least one high-affinity binding site for the Notch-activated transcription factor Suppressor of Hairless, suggesting that all are directly regulated by the Notch pathway. Consistent with this, we show that Bearded family genes are expressed in a variety of territories in imaginal tissue that correspond to sites of active Notch signaling. We demonstrate that overexpression of any family member antagonizes the activity of the Notch pathway in multiple cell fate decisions during adult sensory organ development. These results suggest that Bearded family genes encode a novel class of effectors or modulators of Notch signaling.
Role of dying endothelial cells in transendothelial macromolecular transport. (1990)

There are focal areas in the aorta with an enhanced endothelial permeability to macromolecules, as indicated by the focal uptake of the protein-binding azo dye Evans blue in vivo. These areas exhibit high rates of endothelial cell turnover and a number of structural characteristics in en face endothelial morphology. To determine the relationship of endothelial cell death to macromolecular leakage at the level of individual endothelial cells, thoracic aortas of 12 adult male Sprague-Dawley rats were studied at 3 to 5 minutes after intravenous administration of Evans blue-albumin (EBA). Leakage of EBA around individual endothelial cells in en face preparations of the aorta was visualized by fluorescence microscopy. Dying or dead endothelial cells were identified by indirect immunoglobulin G (IgG) immunocytochemistry. Although endothelial cell death is uncommon in normal aortic endothedium (i.e., an average frequency of 0.48%), a high percentage (63%) of IgG-containing dying or dead endothelial cells was found to be associated with EBA leakage. These dying or dead endothelial cells were responsible for 37% of total EBA leaky foci. The results suggest that, in addition to mitotic endothelial cells, the dying or dead endothelial cells also make significant contributions to the local enhancement in aortic endothelial permeability. The present findings lend further support to the "cell turnover-leaky junction" hypothesis for the localization of atherosclerosis.