gebhart/patrepeval huggingface dataset:
A COMPARISON OF THE CHARACTERISTICS OF M&A ACTIVITY IN FIVE LATIN AMERICAN COUNTRIES AND THE UNITED STATES: 1985–1994 (1997)

The Latin American merger and acquisition (M&amp;A) market offers enormous opportunities. M&amp;A activity in Argentina, Brazil, Chile, Mexico and Venezuela is examined and compared with M&amp;A activity in the United States. Characteristics examined include the industries and form of ownership of the target firms, the types of transactions, and the home countries of the acquiring firms. Differences are found between characteristics of M&amp;A activity in the Latin American countries and the United States. Differences are also found among the characteristics of M&amp;A activity in the various Latin American countries. Implications for managers and areas in need of additional research are discussed.
Inflammation-Based Scores: A New Method for Patient-Targeted Strategies and Improved Perioperative Outcome in Cancer Patients (2014)

Systemic inflammatory response (SIR) has actually been shown as an important prognostic factor associated with lower postoperative survival in several types of cancer. Thus, the challenge for physicians is to find specific, low-cost, and highly reliable inflammatory markers, clearly correlated with prognosis and able to preoperatively stratify patient's risk. Inflammation is a promising target to improve perioperative outcome, and data show that anti-inflammation techniques have a great potential in the perioperative period of cancer surgery. Inflammation scores could be useful to stratify patients with a potential better response to anti-inflammation strategies. Furthermore, inflammation scores could prevent failure of clinical trials by a better definition of patients to be included in such trials; inflammation scoring could clarify the real role of different drugs and techniques on outcome after cancer surgery, defining if different therapies are required for different patients. The role of this review is to focus on the currently available scores, in order to clarify their rationale and to analyze the actual evidence and limits, providing physicians with an updated overview of the possible inflammation-based prognostic scores for cancer patients undergoing surgery.
Synthesis and in Vitro Efficacy of Transferrin Conjugates of the Anticancer Drug Chlorambucil (1998)

One strategy for improving the selectivity and toxicity profile of antitumor agents is to design drug carrier systems employing soluble macromolecules or carrier proteins. Thus, five maleimide derivatives of chlorambucil were bound to thiolated human serum transferrin which differ in the stability of the chemical link between drug and spacer. The maleimide ester derivatives 1 and 2 were prepared by reacting 2-hydroxyethylmaleimide or 3-maleimidophenol with the carboxyl group of chlorambucil, and the carboxylic hydrazone derivatives 5-7 were obtained through reaction of 2-maleimidoacetaldehyde, 3-maleimidoacetophenone, or 3-maleimidobenzaldehyde with the carboxylic acid hydrazide derivative of chlorambucil. The alkylating activity of transferrin-bound chlorambucil was determined with the aid of 4-(4-nitrobenzyl)pyridine (NBP) demonstrating that on average 3 equivalents were protein-bound. Evaluation of the cytotoxicity of free chlorambucil and the respective transferrin conjugates in the MCF7 mammary carcinoma and MOLT4 leukemia cell line employing a propidium iodide fluorescence assay demonstrated that the conjugates in which chlorambucil was bound to transferrin through non-acid-sensitive linkers, i.e., an ester or benzaldehyde carboxylic hydrazone bond, were not, on the whole, as active as chlorambucil. In contrast, the two conjugates in which chlorambucil was bound to transferrin through acid-sensitive carboxylic hydrazone bonds were as active as or more active than chlorambucil in both cell lines. Especially, the conjugate in which chlorambucil was bound to transferrin through an acetaldehyde carboxylic hydrazone bond exhibited IC50 values which were approximately 3-18-fold lower than those of chlorambucil. Preliminary toxicity studies in mice showed that this conjugate can be administered at higher doses in comparison to unbound chlorambucil. The structure-activity relationships of the transferrin conjugates are discussed with respect to their pH-dependent acid sensitivity, their serum stability, and their cytotoxicity.
Blazingly Fast Video Object Segmentation with Pixel-Wise Metric Learning (2018)

This paper tackles the problem of video object segmentation, given some user annotation which indicates the object of interest. The problem is formulated as pixel-wise retrieval in a learned embedding space: we embed pixels of the same object instance into the vicinity of each other, using a fully convolutional network trained by a modified triplet loss as the embedding model. Then the annotated pixels are set as reference and the rest of the pixels are classified using a nearest-neighbor approach. The proposed method supports different kinds of user input such as segmentation mask in the first frame (semi-supervised scenario), or a sparse set of clicked points (interactive scenario). In the semi-supervised scenario, we achieve results competitive with the state of the art but at a fraction of computation cost (275 milliseconds per frame). In the interactive scenario where the user is able to refine their input iteratively, the proposed method provides instant response to each input, and reaches comparable quality to competing methods with much less interaction.
High efficiency and high linearity microwave power amplifiers based on ultra-high f/sub max/ selective buried sub-collector (SBSC) HBTs (2002)

This paper presents several microwave power amplifier designs that promise high power and broad bandwidth, while meeting the efficiency and linearity requirements of advanced radar and communication systems. Power amplifiers are designed based on an ultra-high f/sub max/ (>450 GHz) selective buried sub-collector (SBSC) HBT technology. SBSC HBTs are fabricated on pre-patterned sub-collector mesas. The active device region is carefully designed so that the overlap between the extrinsic base and the sub-collector is totally eliminated for minimizing the base collector capacitance and maximizing the device f/sub max/. Simulations on SBSC HBT microwave amplifiers show that >10 W output power may be achievable with PAE>50% and OIP3=40 dBm at 7-11 GHz (X-band) and >4 W output power with PAE=30% and OIP3=32 dBm at 28-32 GHz (Ka-band). These results show the great potential of using SBSC HBTs for broadband and high power microwave amplifiers with high efficiency and linearity.
Gastric emptying in normal subjects--a reproducible technique using a single scintillation camera and computer system. (1983)

The gastric emptying of a mixed solid and liquid meal was assessed in 24 normal subjects using a single camera/computer system which allowed continuous monitoring of both solids and liquids. It was shown that variation in tissue attenuation caused by the changing depth of radionuclide within the stomach accounted for large errors in the measurement of gastric emptying (alteration in 50% emptying time of up to 65%). A technique for the correction of attenuation is described which used factors derived from a lateral image of the stomach. In all subjects, solid emptying was slower than liquid emptying and was characterised by a delay (lag period) which was followed by linear emptying. Liquid emptying usually followed a single exponential pattern. The effect of physiological changes induced by increasing the calorie content of the liquid component of the meal was assessed by giving either water, 10% dextrose or 25% dextrose. Liquid emptying was slowed and the lag period of solid was prolonged as the calorie content increased. Reproducibility was assessed in 19 subjects. For the three groups studied (water, 10% dextrose, 25% dextrose) the day-to-day variation in gastric emptying was not significant for any measured parameter, while statistically significant differences were present in solid and liquid emptying between subjects and groups.
Wild-type bone marrow cells ameliorate the phenotype of SOD1-G93A ALS mice and contribute to CNS, heart and skeletal muscle tissues (2004)

Amyotrophic lateral sclerosis (ALS) is a progressive, lethal neurodegenerative disease without any effective therapy. To evaluate the potential of wild-type bone marrow (BM)-derived stem cells to modify the ALS phenotype, we generated BM chimeric Cu/Zn superoxide dismutase (SOD1) mice by transplantation of BM cells derived from mice expressing green fluorescent protein (GFP) in all tissues and from Thy1-YFP mice that express a spectral variant of GFP (yellow fluorescent protein) in neurons only. In the recipient cerebral cortex, we observed rare GFP+ and YFP+ neurons, which were probably generated by cell fusion, as demonstrated by fluorescence in situ hybridization (FISH) analysis, suggesting that this phenomenon is not limited to Purkinje cells. GFP-positive microglial cells were extensively present in both the brain and spinal cord of the affected animals. Completely differentiated and immature GFP+ myofibres were also present in the heart and skeletal muscles of SOD1 mice, confirming that BM cells can participate in striated muscle tissue regeneration. Moreover, wild-type BM chimeric SOD1 mice showed a significantly delayed disease onset and an increased life span, probably due to a positive 'non-neuronal environmental' effect rather than to neuronogenesis. This improvement in SOD1-G93A mouse survival is comparable with that previously obtained using some safer pharmacological agents. BM transplantation-related complications in humans preclude its clinical application for ALS treatment. However, our data suggest that further studies aimed at improving the degree of tissue chimerism by BM-derived cells may provide valuable insights into strategies to slow ALS progression.
Gcn4p and Novel Upstream Activating Sequences Regulate Targets of the Unfolded Protein Response (2004)

Eukaryotic cells respond to accumulation of unfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR), a signal transduction pathway that communicates between the ER and the nucleus. In yeast, a large set of UPR target genes has been experimentally determined, but the previously characterized unfolded protein response element (UPRE), an upstream activating sequence (UAS) found in the promoter of the UPR target gene KAR2, cannot account for the transcriptional regulation of most genes in this set. To address this puzzle, we analyzed the promoters of UPR target genes computationally, identifying as candidate UASs short sequences that are statistically overrepresented. We tested the most promising of these candidate UASs for biological activity, and identified two novel UPREs, which are necessary and sufficient for UPR activation of promoters. A genetic screen for activators of the novel motifs revealed that the transcription factor Gcn4p plays an essential and previously unrecognized role in the UPR: Gcn4p and its activator Gcn2p are required for induction of a majority of UPR target genes during ER stress. Both Hac1p and Gcn4p bind target gene promoters to stimulate transcriptional induction. Regulation of Gcn4p levels in response to changing physiological conditions may function as an additional means to modulate the UPR. The discovery of a role for Gcn4p in the yeast UPR reveals an additional level of complexity and demonstrates a surprising conservation of the signaling circuit between yeast and metazoan cells.
Hyperpolarization‐activated, cyclic nucleotide‐gated cation channels: Roles in the differential electrophysiological properties of rat primary afferent neurons (2004)

The large, medium-sized, and small neurons of the dorsal root ganglion (DRG) have different functions in the processing of various senses. Hyperpolarization-activated, cyclic nucleotide-gated channels (HCN) contribute greatly to neuronal excitability. In the present study, which used whole-cell patch clamp techniques and immunohistochemical staining methods, the electrophysiological properties of DRG neurons were systematically compared, and the roles of HCN-1, -2, and -4 were examined. The main results were as follows. 1) The large neurons had significantly higher V0.5 values (membrane potential at which the HCN channels were half-activated) and shorter time constants (tau) than small or medium-sized DRG neurons. However, large DRG neurons had higher Ih density (HCN neuron current). 2) HCN-1 was found predominantly, but not exclusively, in large and medium-sized DRG neurons; HCN-2 was found in all DRG neurons; and HCN-4 was poorly visualized in all DRG neurons. HCN-1 and HCN-2 were colocalized in large and medium-sized neurons with immunostaining of adjacent sections. In the dorsal horn of the spinal cord, HCN-1, HCN-2, and HCN-4 were all expressed in laminae I-IV, although HCN-1 was not detectable in lamina II. 3) Blockade of Ih current in DRG neurons caused a significant decrease in V0.5, resting membrane potential, and repetitive firing number of action potential and a significant increase in time of rising phase of action potential. These results suggest that the different HCN channels in the three types of DRG neurons might contribute to their differential electrophysiological properties.
Manipulation of T-Cell Responses with Monoclonal Antibodies (1989)

Exhausted CD8 T (Tex) cells are a distinct cell lineage that arise during chronic infections and cancers in animal models and humans. Tex cells are characterized by progressive loss of effector functions, high and sustained inhibitory receptor expression, ...Read More
Transgene Expression of bcl-xL Permits Anti-immunoglobulin (Ig)–induced Proliferation in xid B Cells (1998)

Mutations in the tyrosine kinase, Btk, result in a mild immunodeficiency in mice (xid). While B lymphocytes from xid mice do not proliferate to anti-immunoglobulin (Ig), we show here induction of the complete complement of cell cycle regulatory molecules, though the level of induction is about half that detected in normal B cells. Cell cycle analysis reveals that anti-Ig stimulated xid B cells enter S phase, but fail to complete the cell cycle, exhibiting a high rate of apoptosis. This correlated with a decreased ability to induce the anti-apoptosis regulatory protein, Bcl-xL. Ectopic expression of Bcl-xL in xid B cells permitted anti-Ig induced cell cycle progression demonstrating dual requirements for induction of anti-apoptotic proteins plus cell cycle regulatory proteins during antigen receptor mediated proliferation. Furthermore, our results link one of the immunodeficient traits caused by mutant Btk with the failure to properly regulate Bcl-xL.
An Alul RFLP detected in human the human prion protein (PrP) gene (1990)

Source/description: Cf56a is a 0.9 kb EcoRI fragment of dystrophin cDNA in pUC13 (1).Cf56a is identical to Kunkel's cDNA probe 8. Polymorphism: Constant bands of 14.4, 11.0, 8.1, 6.2 and 1.3 kb correspond to exons I, N, L, M and K respectively of ref ( 2).The polymorphic band is exon J (exon 48, 1.2+3.9kb HindHI bands).Frequency: Estimated from 149 chromosomes in caucasian British Allele 1 6.7 kb 0.62 Allele 2 6.4 kb 0.25 Allele 3 4.2 kb 0.13Chromosomal location: Xp2l.Mendelian inheritance: Codominant segregation of all alleles in a large 4-generation family and 10 nuclear families.Probe availability: Contact Kay Davies, Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU (or ATCC for probe 8).Other comments: This exon is deleted in 25% of all Duchenne/Becker dystrophy boys (2).Therefore this RFLP is useful for determining carrier status of at-risk females by showing heterozygosity or apparent non-maternity.
Determination of clonality of metastasis by cell-specific color-coded fluorescent-protein imaging. (2003)

It has been thought that metastases are clonal and originate from rare cells in primary tumors that are heterogeneous in genotype and phenotype. Recent studies using DNA array analysis challenge this hypothesis and suggest the genetic background of the host is the important determinant of metastatic potential implying that metastases are not necessarily clonal. Previous methods to determine clonality of metastasis used karyotype or molecular analysis that were complicated, thereby limiting the number of metastatic colonies analyzed and the conclusions that could be drawn. We describe here the use of green fluorescent protein-labeled or red fluorescent protein-labeled HT-1080 human fibrosarcoma cells to determine clonality by simple fluorescence visualization of metastatic colonies after mixed implantation of the red and green fluorescent cells. Resulting pure red or pure green colonies were scored as clonal, whereas mixed yellow colonies were scored as nonclonal. In a spontaneous metastasis model originating from footpad injection in severe combined immunodeficient mice, 95% of the resulting lung colonies were either pure green or pure red, indicating monoclonal origin, whereas 5% were of mixed color, indicating polyclonal origin. In an experimental lung metastasis model established by tail vein injection in severe combined immunodeficient mice, clonality of lung metastasis was dependent on cell number. With a minimum cell number injected, almost all (96%) colonies were pure red or green and therefore monoclonal. When a large number of cells were injected, almost all (87%) colonies were mixed color and therefore heteroclonal. We conclude that spontaneous metastasis may be clonal because they are rare events, thereby supporting the rare-cell clonal origin of metastasis hypothesis. The clonality of the experimental metastasis model depended on the number of input cells. The simple fluorescence method of determining clonality of metastases described here can allow large-scale clonal analysis in numerous types of metastatic models.
The collapse and reemergence of networks within and between Republics of the Former Soviet Union (1996)

A large body of research explores the characteristics of networks in Western economies. This study primarily analyzes supply networks in Estonia before and after the breakup of the former Soviet Union and the impact of networks on firm performance. Of particular interest is whether firms which had to reestablish networks were better off than those which were able to sustain previously established networks. Significant differences were found in the pre-independence network structures of high and low performing firms. After independence, network structures were not significantly different, with linkages to the former Soviet Union severed for both groups. Successful firms were found to have Estonia-centered customer and supply networks existing before independence, while unsuccessful firms pre-independence networks centered on the Soviet Union.
Industrial applications of temperature and species concentration monitoring using laser diagnostics (2002)

Industrial applications of laser diagnostics have been demonstrated for the purpose of clarifying combustor chemical reaction mechanisms, as well as temperature and harmful substance monitoring in large-scale burners and commercial plant exhaust ducts, and the combustion control of commercial plants. Laser induced fluorescence (LIF), laser induced breakdown spectroscopy (LIBS), and tunable diode laser absorption spectroscopy (TDLAS) have accordingly been applied in various industrial fields. In this study, temperature and species concentration were measured inside gas turbine combustors, a diesel engine, and a large-scale industrial burner using LIF. This technique introduces a new tool with respect to practical combustors for the analysis of NO formation characteristics, turbulent flame front structure, and differences between standard and improved combustors. On-line monitoring of trace elements to the ppb level was also successfully demonstrated using LIBS. The automated LIBS unit was found to be capable of monitoring trace element concentration fluctuations at ppb levels with a 1 min detection time under actual plant conditions. In addition, real-time measurement of O2 and CO concentrations in a commercial incinerator furnace was performed using TDLAS to improve the combustion control. By using the multiple-point laser measurement results to control secondary air allocation, higher secondary combustion efficiency was achieved, and CO concentration (considered to be a substitute indicator for dioxins) was reduced from 11.9 to 8.0 ppm.
Multiple Epitopes on Cartilage Type II Collagen are Accessible for Antibody Binding in vivo (1991)

Monoclonal mouse antibodies specific for the major epitopes on mouse type II collagen (CII) were biotinylated and injected into neonatal and adult mice. Anti-CII antibodies, specific for four different epitopes on the CII molecule, could be shown to bind specifically to joint surfaces in the paws of 2-day-old syngeneic DBA/I mice after an intraperitoneal injection of 100 μg of biotinylated antibody. The anti-CII antibodies did not bind to cartilage from DBA/I mice in vitro, unless the sections were pretreated with hyaluronidase or the specimens decalcified prior to freezing, showing that the epitopes are accessible in vivo but not in vitro. By analyzing the in vivo binding capacity for a number of monoclonal anti-CII antibodies which represented different IgG subclasses, it could be demonstrated that binding to the same epitopes occurred independent of IgG subclass. However, one epitope (denoted “BI”) was only weakly detected, possibly due to the fact that the antibody used (CIIBI) crossreacts with type 1 collagen and Clq. Monoclonal anti-CII antibodies, injected into neonates or adult mice, bound specifically to most, but not all, tissues containing CII; including hyaline joint cartilage, fibrous sternal and costal cartilage, tracheal cartilage and fibrous cartilage in the spine but not to CII-containing structures in the eye. The finding that CII, while present in cartilage, is accessible for antibody binding in vivo may have important implications for the availability of CII for the immune system and for the understanding of the development of pathological autoimmunity leading to collagen-induced arthritis in mice.
Synthesis and binding properties of pyrimidine oligodeoxynucleoside analogs containing neutral phosphodiester replacements: the formacetal and 3'-thioformacetal internucleoside linkages (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and binding properties of pyrimidine oligodeoxynucleoside analogs containing neutral phosphodiester replacements: the formacetal and 3'-thioformacetal internucleoside linkagesRobert J. Jones, Kuei Ying Lin, John F. Milligan, Shalini Wadwani, and Mark D. MatteucciCite this: J. Org. Chem. 1993, 58, 11, 2983–2991Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/jo00063a014https://doi.org/10.1021/jo00063a014research-articleACS PublicationsRequest reuse permissionsArticle Views342Altmetric-Citations92LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts