gebhart/patrepeval huggingface dataset:
ATP released from cardiac fibroblasts via connexin hemichannels activates profibrotic P2Y 2 receptors (2012)

Cardiac fibroblasts (CFs) play an essential role in remodeling of the cardiac extracellular matrix. Extracellular nucleotide signaling may provoke a profibrotic response in CFs. We tested the hypothesis that physical perturbations release ATP from CFs and that ATP participates in profibrotic signaling. ATP release was abolished by the channel inhibitor carbenoxolone and inhibited by knockdown of either connexin (Cx)43 or Cx45 (47 and 35%, respectively), implying that hypotonic stimulation induces ATP release via Cx43 and Cx45 hemichannels, although pannexin 1 may also play a role. ATP released by hypotonic stimulation rapidly (<10 min) increased phosphorylated ERK by 5-8 fold, an effect largely eliminated by P2Y(2) receptor knockdown or ATP hydrolysis with apyrase. ATP stimulation of P2Y(2) receptors increased α-smooth muscle actin (α-SMA) production, and in an ERK-dependent manner, ATP increased collagen accumulation by 60% and mRNA expression of profibrotic markers: plasminogen activator inhibitor-1 and monocyte chemotactic protein-1 by 4.5- and 4.0-fold, respectively. Apyrase treatment substantially reduced the basal profibrotic phenotype, decreasing collagen and α-SMA content and increasing matrix metalloproteinase expression. Thus, ATP release activates P2Y(2) receptors to mediate profibrotic responses in CFs, implying that nucleotide release under both basal and activated states is likely an important mechanism for fibroblast homeostasis.
Displaced subdivision surfaces (2000)

In this paper we introduce a new surface representing, the displaced subdivision surface. It represents a detailed surface model as a scalar-valued displacement over a smooth domain surface. Our representation defines both the domain surface and the displacement function using a unified subdivision framework, allowing for simple and efficient evaluation of analytic surface properties. We present a simple, automatic scheme for converting detailed geometric models into such a representation. The challenge in this conversion process is to find a simple subdivision surface that still faithfully expresses the detailed model as its offset. We demonstrate that displaced subdivision surfaces offer a number of benefits, including geometry compression, editing, animation, scalability, and adaptive rendering. In particular, the encoding of fine detail as a scalar function makes the representation extremely compact.
Paper Machine Clothing: Key to the Paper Making Process (1997)

Everyone involved in paper making knows Asten as a world class manufacturer of paper machine clothing. Perhaps less well known is that Asten started in this industry more than 120 years ago. Since then the company has taken advantage of modern manufacturing techniques to produce innovative products needed by the growing paper making industry. That is why Asten commissioned Dr. Sabit Adanur to write this book - to continue spreading sophisticated papermaking knowledge throughout the global paper industry. This book discusses how the latest technological innovations help produce quality paper products. It also covers the use of TQM and computers in the papermaking process as basic paper structure and properties.
The Promise of Global Cervical-Cancer Prevention (2005)

Cervical cancer remains a leading form of cancer among women living in low-resource regions of the world. Drs. Mark Schiffman and Philip Castle discuss the promising new prevention strategies.
Dual-mode transport of penetrants in glassy polymers (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDual-mode transport of penetrants in glassy polymersGlenn H. Fredrickson and Eugene HelfandCite this: Macromolecules 1985, 18, 11, 2201–2207Publication Date (Print):November 1, 1985Publication History Published online1 May 2002Published inissue 1 November 1985https://pubs.acs.org/doi/10.1021/ma00153a024https://doi.org/10.1021/ma00153a024research-articleACS PublicationsRequest reuse permissionsArticle Views326Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Galectin-1 Modulates Human Glioblastoma Cell Migration into the Brain Through Modifications to the Actin Cytoskeleton and Levels of Expression of Small GTPases (2002)

We show that high-grade astrocytic tumors with high levels of galectin-1 expression are associated with dismal prognoses. The immunohistochemical analysis of galectin-1 expression of human U87 and U373 glioblastoma xenografts from the brains of nude mice revealed a higher level of galectin-1 expression in invasive areas rather than non-invasive areas of the xenografts. Nude mice intracranially grafted with U87 or U373 cells constitutively expressing low levels of galectin-1 (by stable transfection of an expression vector containing the antisense mRNA of galectin-1) had longer survival periods than those grafted with U87 or U373 cells expressing normal levels of galectin-1. Galectin-1 added to the culture media markedly and specifically increased cell motility levels in human neoplastic astrocytes. These effects are related to marked modifications in the organization of the actin cytoskeleton and the increase in small GTPase RhoA expression. All the data obtained indicate that galectin-1 enhances the migratory capabilities of tumor astrocytes and, therefore, their biological aggressiveness.
A data mining driven risk profiling method for road asset management (2013)

Road surface skid resistance has been shown to have a strong relationship to road crash risk, however, applying the current method of using investigatory levels to identify crash prone roads is problematic as they may fail in identifying risky roads outside of the norm. The proposed method analyses a complex and formerly impenetrable volume of data from roads and crashes using data mining. This method rapidly identifies roads with elevated crash-rate, potentially due to skid resistance deficit, for investigation. A hypothetical skid resistance/crash risk curve is developed for each road segment, driven by the model deployed in a novel regression tree extrapolation method. The method potentially solves the problem of missing skid resistance values which occurs during network-wide crash analysis, and allows risk assessment of the major proportion of roads without skid resistance values.
Large-scale neural networks implemented with non-volatile memory as the synaptic weight element: Comparative performance analysis (accuracy, speed, and power) (2015)

We review our work towards achieving competitive performance (classification accuracies) for on-chip machine learning (ML) of large-scale artificial neural networks (ANN) using Non-Volatile Memory (NVM)-based synapses, despite the inherent random and deterministic imperfections of such devices. We then show that such systems could potentially offer faster (up to 25×) and lower-power (from 120-2850×) ML training than GPU-based hardware.
Autofluorescence of Fungi: An Aid to Detection in Tissue Sections (1983)

Many pathogenic fungi have been found to autofluoresce in routine hematoxylin and eosin sections when exposed to ultraviolet light. These fungi include Blastomyces, Cryptococcus, Candida, Aspergillus, Coccidioides, and occasionally, Histoplasma. No autofluorescence was observed with Mucor. Examinations of other inflammatory processes, including those producing a granulomatous response, have not revealed any false-positive results. The advantages of fluorescent microscopy for fungal screening include: (1) no special staining procedures required; (2) no time delay, as involved with special stains; (3) the ability to scan sections at a relatively low power; and (4) the ability to tentatively identify the fungus. Disadvantages may include: (1) lack of a fluorescent microscope, and (2) a possible aging process whereby autofluorescence is lost in older cases.
Absence of human papillomavirus type 16 E6 transcripts in HPV 16‐infected, cytologically normal cervical scrapings (1993)

By a combination of reversed transcription and subsequent polymerase chain reaction (RNA-PCR), 23 cytologically normal cervical scrapings, positive for the presence of human papillomavirus type 16 (HPV 16) DNA, were analyzed for the presence of transcripts originating from the E6 region of the viral genome. This region is thought to be involved in transformational, tumorigenic events. No mRNAs of the E6 region were detectable using the most sensitive PCR-mediated procedure currently available. Since it was previously shown that in cytological abnormal cervical scrapings about one-half of the samples positive for HPV 16 DNA express mRNAs of the E6 region, a difference between normal and abnormal cervical scrapings, when the HPV 16 is present, exists. The observed difference between cytologically normal and abnormal, HPV-DNA-positive cervical scrapes may eventually be used as a prognostic marker for screening of women at risk for the development of cervical carcinoma. However, firm establishment of the putative correlation between tumor progression and the presence of E6 transcripts requires extensive follow-up analysis of HPV-positive patients.
Discovering drugs to treat coronavirus disease 2019 (COVID-19) (2020)

The SARS-CoV-2 virus emerged in December 2019 and then spread rapidly worldwide, particularly to China, Japan, and South Korea. Scientists are endeavoring to find antivirals specific to the virus. Several drugs such as chloroquine, arbidol, remdesivir, and favipiravir are currently undergoing clinical studies to test their efficacy and safety in the treatment of coronavirus disease 2019 (COVID-19) in China; some promising results have been achieved thus far. This article summarizes agents with potential efficacy against SARS-CoV-2.
Characterization of the myeloid-specific CD11b promoter (1992)

The CD11b/CD18 heterodimeric surface antigen is expressed exclusively on human monocytes, macrophages, granulocytes, and natural killer cells. During differentiation of myeloid cell lines, CD11b steady state messenger RNA levels increase significantly; we show here that CD11b transcription rates increase commensurately. A 1.7-kb fragment of CD11b 5′ flanking sequence directs expression of a reporter gene specifically in myeloid cell lines. Deletion analysis localizes elements directing high levels of tissue-specific reporter gene expression to the 412 bp proximal to the transcriptional start site. This sequence contains two consensus binding sites for Sp1, a GATA motif, and a purine-rich sequence that presents potential binding sites for members of the ets family of genes. Analysis of this promoter should result in the isolation of myeloid-specific transcription factors and the development of methods to direct the myeloid-specific expression of heterologous genes.
Comparative evaluation of incretin‐based antidiabetic medications and alternative therapies to be added to metformin in the case of monotherapy failure† (2010)

Based on the results from clinical trials, incretin-based medications have been shown to be efficacious antidiabetic drugs with a favourable adverse event and tolerability profile. This leads to high scores using a novel system paying attention to multiple facets contributing to the selection of antidiabetic drugs for general recommendation and individual treatment choices.
Fingerprint Based Gender Classification (2024)

Male-female classification from a fingerprint is an important step in forensic science, anthropological and medical studies to reduce the efforts required for searching a person.The aim of this research is to establish a relationship between gender and the fingerprint using some special features such as ridge density, ridge thickness to valley thickness ratio (RTVTR) and ridge width.showed that male-female classification can be done correctly up to 88.5% based on white lines count, RTVTR & ridge count using Neural Network as Classifier.We have used RTVTR, ridge width and ridge density for classification and SVM as classifier.We have found male-female can be correctly classified up to 91%.Gender classification plays an active role in several applications such as biometrics, criminology, surveillance, human computer interaction, commercial profiling.Though biometric traits such as face, gait, iris and hand shape are used for gender classification in the past, majority of the work is based on face as it contains more prominent features than others.In this paper we have analyzed fingerprints for gender classification with a hope that it has great potential for future research.We have employed a three convolutional layer CNN with rectified linear and activation functions on NIST database which contains a set of 4000 images and achieved 99% accuracy.Performance of the proposed system demonstrated that fingerprints contains vital features to discriminate gender of a person.Humans have distinctive and unique traits which can be used to distinguish them thus, acting as a form of identification.Biometrics identify people by measuring some aspect of individual's anatomy or physiology such as hand geometry or fingerprint which consists of a pattern of interleaved ridges and valleys.The year 2015 election in Nigeria was greeted by some petitions including under-aged voters.The need for an age and gender detector system is a major concern for organizations at all levels where integrity of information cannot be compromised.This work developed a system that determines human age-range and gender using fingerprint analysis trained with Back Propagation Neural Network (for gender classification) and DWT+PCA (for age classification).A total of 280 fingerprint samples of people with various age and gender were collected.140 of these samples were used for training the system"s Database; 70 males and 70 females respectively.This was done for age groups 1-10, 11-20, 21-30, 31-40, 41-50, 51-60 and 61-70 accordingly.In order to determine the gender of an individual, the Ridge Thickness Valley Thickness Ratio (RTVTR) of the person was put into consideration.Result showed 80.00 % classification accuracy for females and 72.86 % for males while 115 subjects out of 140 (82.14%)were correctly classified in age.
Peptide Antisera to Human Colony-Stimulating Factor 1 Receptor Detect Ligand-Induced Conformational Changes and a Binding Site for Phosphatidylinositol 3-Kinase (1991)

A peptide antiserum (anti-A) directed to the intracellular, juxtamembrane region (residues 552 to 574) of the human colony-stimulating factor 1 receptor (CSF-1R) precipitated only ligand-activated, native receptors from solution but bound to unstimulated forms after their denaturation. Two peptide antisera (anti-KI1 and -KI2), directed to residues 679 to 700 and 701 to 721, respectively, in the CSF-1R kinase insert (KI) domain and including mapped sites of ligand-induced phosphorylation at Tyr-699 and Tyr-708, bound at least 80% of the receptor molecules expressed in either CSF-1-stimulated or unstimulated cells. Immune complexes formed with anti-KI1, anti-A, or a peptide antiserum to the CSF-1R carboxyl terminus (anti-C-ter) coprecipitated CSF-1R complexed to a phosphatidylinositol 3-kinase (PtdIns 3-K) from CSF-1-stimulated cells, whereas anti-KI2 serum did not. In an in vitro assay, binding of CSF-1R to PtdIns 3-K required receptor tyrosine phosphorylation but not CSF-1R-mediated phosphorylation of the lipid kinase, and the association was specifically blocked by anti-KI2 or antibodies to phosphotyrosine. Neither anti-KI1, anti-A, nor anti-C-ter serum inhibited binding. We conclude that (i) only a minority of ligand-activated receptors form a stable complex with PtdIns 3-K in vivo, (ii) efficient binding of the lipid kinase requires receptor tyrosine phosphorylation within the CSF-1R KI domain, and (iii) a region within the KI domain defined by residues 701 to 721 at least partially overlaps the PtdIns 3-K binding site.
Cell-Penetrating Peptide-Mediated Delivery of TALEN Proteins via Bioconjugation for Genome Engineering (2014)

Transcription activator-like (TAL) effector nucleases (TALENs) have enabled the introduction of targeted genetic alterations into a broad range of cell lines and organisms. These customizable nucleases are comprised of programmable sequence-specific DNA-binding modules derived from TAL effector proteins fused to the non-specific FokI cleavage domain. Delivery of these nucleases into cells has proven challenging as the large size and highly repetitive nature of the TAL effector DNA-binding domain precludes their incorporation into many types of viral vectors. Furthermore, viral and non-viral gene delivery methods carry the risk of insertional mutagenesis and have been shown to increase the off-target activity of site-specific nucleases. We previously demonstrated that direct delivery of zinc-finger nuclease proteins enables highly efficient gene knockout in a variety of mammalian cell types with reduced off-target effects. Here we show that conjugation of cell-penetrating poly-Arg peptides to a surface-exposed Cys residue present on each TAL effector repeat imparted cell-penetrating activity to purified TALEN proteins. These modifications are reversible under reducing conditions and enabled TALEN-mediated gene knockout of the human CCR5 and BMPR1A genes at rates comparable to those achieved with transient transfection of TALEN expression vectors. These findings demonstrate that direct protein delivery, facilitated by conjugation of chemical functionalities onto the TALEN protein surface, is a promising alternative to current non-viral and viral-based methods for TALEN delivery into mammalian cells.
Simultaneous highlighting of paper and electronic documents (2002)

The ability to automatically record the marks applied to paper documents on their electronic originals would preserve the information represented by those annotations. Users could even lose the original paper document. The marked-up version could be regenerated by merely reprinting it. We describe a solution that saves an electronic representation for the highlights users commonly apply over the top of machine-printed text. A unique combination of algorithms is presented that maps the image captured from a pen scanner affixed to a highlighting pen onto text strings in electronic documents. Documents are automatically located in a large database using characteristics of the highlighted text. We describe here the system components, including the image recognition algorithms, and discuss their performance in finding a unique mapping from an image of text onto a sequence of words in an electronic document within a large database.
Cross-Presentation of the Oncofetal Tumor Antigen 5T4 from Irradiated Prostate Cancer Cells—A Key Role for Heat-Shock Protein 70 and Receptor CD91 (2015)

Immune responses contribute to the success of radiotherapy of solid tumors; however, the mechanism of triggering CD8(+) T-cell responses is poorly understood. Antigen cross-presentation from tumor cells by dendritic cells (DC) is a likely dominant mechanism to achieve CD8(+) T-cell stimulation. We established a cross-presentation model in which DCs present a naturally expressed oncofetal tumor antigen (5T4) from irradiated DU145 prostate cancer cells to 5T4-specific T cells. The aim was to establish which immunogenic signals are important in radiation-induced cross-presentation. Radiation (12 Gy) caused G2-M cell-cycle arrest and cell death, increased cellular 5T4 levels, high-mobility protein group-B1 (HMGB1) release, and surface calreticulin and heat-shock protein-70 (Hsp70) expression in DU145 cells. DCs phagocytosed irradiated tumor cells efficiently, followed by upregulation of CD86 on phagocytic DCs. CD8(+) 5T4-specific T cells, stimulated with these DCs, proliferated and produced IFNγ. Inhibition of HMGB1 or the TRIF/MyD88 pathway only had a partial effect on T-cell stimulation. Unlike previous investigators, we found no evidence that DCs carrying Asp299Gly Toll-like receptor-4 (TLR4) single-nucleotide polymorphism had impaired ability to cross-present tumor antigen. However, pretreatment of tumor cells with Hsp70 inhibitors resulted in a highly statistically significant and robust prevention of antigen cross-presentation and CD86 upregulation on DCs cocultured with irradiated tumor cells. Blocking the Hsp70 receptor CD91 also abolished cross-presentation. Together, the results from our study demonstrate that irradiation induces immunologically relevant changes in tumor cells, which can trigger CD8(+) T-cell responses via a predominantly Hsp70-dependent antigen cross-presentation process.
5-Arylthiopyrimidines. III. Cyclization of 4-Hydroxy Derivatives to 10H-Pyrimido[5,4-b][1,4]benzothiazines (1,3-Diazaphenothiazines)1 (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT5-Arylthiopyrimidines. III. Cyclization of 4-Hydroxy Derivatives to 10H-Pyrimido[5,4-b][1,4]benzothiazines (1,3-Diazaphenothiazines)1Barbara Roth and Lorraine A. SchloemerCite this: J. Org. Chem. 1963, 28, 10, 2659–2672Publication Date (Print):October 1, 1963Publication History Published online1 May 2002Published inissue 1 October 1963https://pubs.acs.org/doi/10.1021/jo01045a042https://doi.org/10.1021/jo01045a042research-articleACS PublicationsRequest reuse permissionsArticle Views288Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Prospective Benefits from Cotton Subsidy Cuts and New Technologies (2008)

While waiting for the removal of cotton subsidies, farmers can benefit from the adoption of GM cotton varieties currently available to them, as the experiences of China and India indicate.
Synthesis of Silicon Analogues of Acyclonucleotides Incorporable in Oligonucleotide Solid-Phase Synthesis (1997)

The synthesis of the four silicon analogues of acyclonucleosides was described. In every case, the silicon atom was introduced onto an allyl group on the natural nucleobase following a hydrosilylation reaction. Diols obtained were protected as 4,4‘-dimethoxytrityl ethers and subsequently activated as 2-cyanoethyl N,N-diisopropylchlorophosphoramidite in order to be suitable for oligonucleotide solid phase synthesis.