gebhart/patrepeval huggingface dataset:
Photonic bandgap fibers exploiting omnidirectional reflectivity enable flexible delivery of infrared lasers for tissue cutting (2004)

Laser cutting of human bone and tissue is one of the oldest and most widespread applications of biophotonics. Due to the unique absorption of different kinds of tissue, choosing an appropriate laser wavelength allows selective ablation of tissue. Consequently, a large variety of laser sources with different emission wavelengths have been successfully applied to an equally large variety of medical indications. However, only a limited set of successful tissue-interaction experiments have translated into standard minimally-invasive procedures. One of the main reasons for this discrepancy between medical research and clinical practice is the lack of a commercially viable, flexible, and easy-to-use fiber optic beam delivery systems for wavelengths longer than 2 &mu;m. In this paper, we will show how OmniGuide fibers, a new type of photonic bandgap fibers, could solve this problem. Recent performance data will be presented for both straight and bent fibers, including losses and power capacity, for delivery of CO<sub>2</sub> lasers. We will also highlight medical procedures where these fibers could find first applications.
3D rasterization: a bridge between rasterization and ray casting (2012)

Ray tracing and have long been considered as two fundamentally different approaches to rendering images of scenes, although they compute the same results for primary rays. Rasterization projects every triangle onto the image plane and enumerates all covered pixels in 2D, while ray tracing operates in by generating rays through every pixel and then finding the closest intersection with a triangle. In this paper we introduce a new view on the two approaches: based on the Plucker ray-triangle intersection test, we define triangle edge functions, resembling (homogeneous) 2D edge functions. Then both approaches become identical with respect to coverage computation for image samples (or primary rays). This generalized 3D rasterization perspective enables us to exchange concepts between both approaches: we can avoid applying any model or view transformation by instead transforming the sample generator, and we can also eliminate the need for perspective division and render directly to non-planar viewports. While ray tracing typically uses floating point with its intrinsic numerical issues, we show that it can be implemented with the same consistency rules as 2D rasterization. With the only remaining differences between the two approaches are the scene traversal and the enumeration of potentially covered samples on the image plane (binning). allows us to explore the design space between traditional and ray casting in a formalized manner. We discuss performance/cost trade-offs and evaluate different implementations and compare to traditional ray tracing and 2D rasterization.
Native-oxide-defined coupled-stripe AlxGa1−xAs-GaAs quantum well heterostructure lasers (1991)

Data are presented on the continuous-wave (cw) room-temperature (300 K) operation of multiple stripe AlxGa1−xAs-GaAs quantum well heterostructure (QWH) laser arrays defined with native oxide contact masking. Use of the native AlxGa1−xAs(x≳0.7) oxide allows the fabrication of high-performance devices without depositing foreign oxide or dielectric layers (SiO2 or Si3N4). Arrays of ten 5-μm-wide emitters on 7 μm centers are coupled and operate at powers as high as 300 mW per facet, or at wider stripe spacing (5 μm emitters on 10 μm centers) as high as 400 mW per facet. These data indicate that current blocking layers of native oxide, formed from AlxGa1−xAs with H2O vapor in N2 carrier gas (400 °C, 3 h), can be used in the construction of high-power multiple stripe QWH arrays with excellent performance characteristics.
Therapeutic potential of a tumor-specific, MHC-unrestricted T-cell receptor expressed on effector cells of the innate and the adaptive immune system through bone marrow transduction and immune reconstitution (2005)

T-cell receptor (TCR) with unique major histocompatibility complex (MHC)-unrestricted antigen-binding properties was isolated from a human T-cell clone specific for the tumor antigen MUC1. This TCR binds its epitope on the MUC1 protein without the requirement of processing and presentation. A single-chain Valpha/Vbeta/Cbeta (scTCR) was fused to a CD3 zeta (zeta) chain to allow expression on the surface of cells of the innate (granulocytes, macrophages, natural killer [NK] cells) as well as the adaptive (T and B cells) immune system. To test the ability of the cells of the innate immune system to reject a tumor when provided with a tumor antigen-specific TCR, we reconstituted severe combined immunodeficiency (SCID) mice with bone marrow cells transduced with a retroviral vector encoding this receptor and challenged them with a MUC1-positive human tumor. These mice controlled the growth of the tumor significantly better than the control mice. We performed a similar experiment in immunocompetent mice transgenic for human MUC1. Expression of the TCR on large percentages of cells did not result in infiltration or destruction of tissues expressing MUC1. Reconstituted mice controlled the outgrowth of a MUC1-transfected but not the parental control tumor. scTCR expression appears lifelong, suggesting a successful transduction of the self-renewing stem cells.
Salmonella penetration of egg shells and proliferation in broiler hatching eggs–a review (2000)

The presence of salmonellae in fertile broiler hatching eggs has been clearly identified as a critical control point in the salmonellae contamination of broiler chickens. This paper reviews the published research studies on a) the penetration and proliferation of salmonellae in hatching eggs, b) the consequences of this contamination on the contamination of the final product, and c) the egg's defenses against invading salmonellae. A better understanding of the material in this review paper will assist poultry researchers and the poultry industry in continuing to make progress in reducing and eliminating salmonellae from fertile hatching eggs, hatcheries, and breeder flocks.
Cytokine activity in cancer-related anorexia/cachexia: role of megestrol acetate and medroxyprogesterone acetate. (1998)

The characteristic clinical picture of anorexia, tissue wasting, loss of body weight accompanied by a decrease in muscle mass and adipose tissue, and poor performance status that often precedes death has been named the cancer-related anorexia/cachexia syndrome (CACS). Chronic administration of pro-inflammatory cytokines, including interleukin-I (IL-I), IL-6, and tumor necrosis factor (TNF), either alone or in combination, is capable of reproducing the different features of CACS. High serum levels of these cytokines have been found in cancer patients, which seem to correlate with progression of the tumor. This article describes a series of experimental and clinical studies demonstrating that: (1) high serum levels of some cytokines, including IL-I, IL-6, and TNF, are present in advanced-stage cancer patients, particularly those with CACS; (2) megestrol acetate (MA) has a beneficial therapeutic effect on CACS symptoms, such as appetite, body weight, and quality of life; (3) MA downregulates the synthesis and release of cytokines and relieves the symptoms of CACS; (4) cytokines play a key role in the onset of CACS; (5) medroxyprogesterone acetate (MPA) reduces the in vitro production of cytokines and serotonin (5-hydroxytryptamine, 5-HT) by peripheral blood mononuclear cells (PBMC) of cancer patients; and (6) MA and MPA reduce the cisplatin-induced 5-HT release in vitro from PBMC of cancer patients. Based on these results, a clinical study incorporating MA/MPA in combination with chemotherapy or chemoimmunotherapy may be warranted. In addition, it has been recently shown that "oxidative stress" resulting from reactive oxygen species, which can be induced by pro-inflammatory cytokines, is involved in tissue wasting and CACS. These results suggest promising approaches for the prevention and treatment of cytokine-induced CACS based on MA, MPA, and metabolic antioxidants.
Genomics of Smoking Exposure and Cessation: Lessons for Cancer Prevention and Treatment (2008)

Tobacco use is the greatest preventable cause of cancer in the United States, accounting for almost one third of all cancerrelated deaths and 90% of deaths from lung cancer. Despite widespread knowledge of these risks, tobacco use prevalence rates are 20% in the United States and up to 30% to 50% in the developing world (1, 2). Nicotine, the addictive chemical in tobacco, produces a biological dependence (3), and therefore, even with the most efficacious medications available, only one in four smokers is able to maintain long-term abstinence (4). Persistent tobacco use is common even following a diagnosis of tobaccorelated cancer and is associated with poorer outcomes of radiation therapy and chemotherapy and with increased risk of second primary malignancies (5). Although smoking cessation is optimal, it may not be realistic for all patients. Therefore, it is essential to gain a better understanding of the cellular and molecular mechanisms through which tobacco exposure contributes to cancer pathogenesis and outcomes, and to develop targeted prevention and therapeutic approaches. Toward this end, a study reported by Gumus et al. (6) in this issue of the journal characterized gene expression profiles in the oral mucosa of smokers and nonsmokers and validated their findings by examining the in vitro effects of tobacco smoke condensate on gene expression in an oral leukoplakia cell line. Another related study also reported in this issue of the journal was conducted by Zhang et al. (7), who compared gene expression in cells obtained from bronchial brushes from never, current, and former smokers. Smoking effects on global gene expression were common across these studies. Certain genes (CYP1A1 and CYP1B1) that were up-regulated in buccal oral specimens of smokers were part of a larger group (CYP1A1, CYP1B1, ALDH3A1, NQO1, and AKR1C1) up-regulated following in vitro exposure to tobacco smoke condensate (6) and in the airway cells of smokers (7). Zhang and colleagues provide further in vivo evidence that many of these genes are down-regulated in persons who reported quitting smoking at least 1 year before the assessment (CYP1B1, AKR1C1, AKR1C2, AKR1B10 ,a ndALDH3A1). Many of these same genes were previously shown to be overexpressed in oral cancer cells exposed to tobacco smoke condensate (8), in bronchial epithelial cells of smokers, and in patients' non–small cell lung carcinoma cells (9, 10). The effects are also consistent with the effects of smoking on the airway transcriptome (11, 12). Taken together, these findings identify important tobacco smoke exposure response genes. The studies of Gumus et al. and Zhang et al. in this issue of the journal provide an elegant illustration of the value of cross-model (preclinical and clinical) validation in translational cancer prevention research.
Catalysis with Transition Metal Nanoparticles in Colloidal Solution:  Nanoparticle Shape Dependence and Stability (2005)

While the nanocatalysis field has undergone an explosive growth during the past decade, there have been very few studies in the area of shape-dependent catalysis and the effect of the catalytic process on the shape and size of transition metal nanoparticles as well as their recycling potential. Metal nanoparticles of different shapes have different crystallographic facets and have different fraction of surface atoms on their corners and edges, which makes it interesting to study the effect of metal nanoparticle shape on the catalytic activity of various organic and inorganic reactions. Transition metal nanoparticles are attractive to use as catalysts due to their high surface-to-volume ratio compared to bulk catalytic materials, but their surface atoms could be so active that changes in the size and shape of the nanoparticles could occur during the course of their catalytic function, which could also affect their recycling potential. In this Feature Article, we review our work on the effect of the shape of the colloidal nanocatalyst on the catalytic activity as well as the effect of the catalytic process on the shape and size of the colloidal transition metal nanocatalysts and their recycling potential. These studies provide important clues on the mechanism of the reactions we studied and also can be very useful in the process of designing better catalysts in the future.
Antennas and propagation: a telecommunications systems subject (1988)

The design, construction, testing and utilisation of communication systems between a source and a sink physically well apart, i.e. telecommunications, involves a variety of signal processing stages. Two of these are the channel encoding and decoding which contain the fundamental aspects of modulation and demodulation with their wealth of varieties, particularly, since the expansion of digital communications. There are two other important elements of the channel encoding/decoding: the antennas or transducers between transmitter and receiver, and the channel or propagation medium. The telecommunications/microwave systems engineering course taught at Portsmouth Polytechnic is outlined.
Identification of activated tumor antigen-reactive CD8+ cells in healthy individuals. (2000)

We investigated the ability of HER-2 peptide E75, which maps an immunodominant CTL epitope for ovarian and breast tumor-associated lymphocytes (TAL), to activate effector functions in freshly isolated CD8+ cells from healthy individuals. IFN-gamma was rapidly induced by E75 within 20-24 h, in five of six healthy donors, in the presence of IL-12 and was detectable as early as 6 h. The IFN-gamma levels were Ag-concentration dependent. Similar results were obtained with peptides mapping CTL epitopes from two other tumor Ag: folate binding protein (FBP) and amino-enhancer of split of Notch (AES). IFN-gamma was also detected, from freshly isolated, unstimulated PBMC in response to HLA-A2 matched tumors + IL-12 but not of IL-12 alone. The major source of IFN-gamma were CD45RO+ CD8+ cells. Induction of IFN-gamma and IL-2 from CD8+ cells and of IL-12 from dendritic cells (DC) by CD8+ cells reactive with E75 mirrored their induction by the influenza matrix peptide (M1: 58-66) in the same individual. Responses to M1 are used to define the presence of activated memory cells in healthy individuals. Compared to M1 responses E75 recognition induced 2-4-fold lower levels of IL-12 from the same APC and IFN-gamma and IL-2 from the same CD8+ cells. At lower Ag concentrations the endogenous IL-12 induced by E75-reactive CD8+ cells did not reach the threshold required to co-stimulate for IFN-gamma. alphaB7.1 synergized with E75 in increasing the overall levels of IL-2 induced within 24 h. The presence of tumor Ag-reactive activated CD8+ cells in healthy individuals may improve our understanding of the mechanisms of immunosurveillance and regulation of immune responses by tumors.
Influence of the incorporation of (S)-9-(3,4-dihydroxybutyl) adenine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides (1991)

(S)-9-(3,4-dihydroxybutyl)adenine was used at several positions as nucleoside substitute in the synthesis of dimers and 13-mers. Therefore we used the phosporamidite and the H-phosphonate chemistry. The nuclease susceptibilities and the base-pairing properties of these oligomers have been evaluated.
Boronic acids selectively facilitate glucose transport through a lipid bilayer (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBoronic acids selectively facilitate glucose transport through a lipid bilayerPamela R. Westmark and Bradley D. SmithCite this: J. Am. Chem. Soc. 1994, 116, 20, 9343–9344Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/ja00099a067https://doi.org/10.1021/ja00099a067research-articleACS PublicationsRequest reuse permissionsArticle Views532Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Utilization of Health Care Services in Patients With Severe Obstructive Sleep Apnea (1996)

We compared the health care utilization of 97 obese patients diagnosed with obstructive sleep apnea (OSA) and 97 matched control subjects. Over a 2-year period that ended 2 years prior to initial diagnosis, the OSA group had 251 nights in hospital, compared to 90 nights for the control group. During the same 2-year period, total expenditures from physician claims were $82,238 (Canadian dollars) in the OSA patients versus $41,018 in the control group (p < 0.01). Depending upon which assumptions one uses for the calculation of hospital costs, during the same 2-year period, the 97 OSA patients utilized between $100,000 and $200,000 more in services than their control counterparts. We conclude that sleep apnea patients are already heavy consumers of health care services prior to any specific evaluation and treatment for apnea.
PolarX Cryoballoon metrics predicting successful pulmonary vein isolation: targets for ablation of atrial fibrillation (2022)

The PolarX Cryoballoon has a different cryoablation profile to AFA Cryoballoon. Prospective testing of these proposed targets in large outcomes studies is required.
Medical Diagnosis: From Student to Clinician (1984)

This book is about the way medical students and doctors think when they are trying to make a diagnosis. It is also about the effects that medical education and clinical experience have on that thinking.
X-ray Structures of Human Neutrophil Collagenase Complexed with Peptide Hydroxamate and Peptide Thiol Inhibitors. Implications for Substrate Binding and Rational Drug Design (1995)

Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases involved in tissue remodeling. They have also been implicated in various disease processes including tumour invasion and joint destruction and are therefore attractive targets for inhibitor design. For rational drug design, information of inhibitor binding at the atomic level is essential. Recently, we have published the refined high-resolution crystal structure of the catalytic domain of human neutrophil collagenase (HNC) complexed with the inhibitor Pro-Leu-Gly-NHOH, which is a mimic for the unprimed (P3-P1) residues of a bound peptide substrate. We have now determined two additional HNC complexes formed with the thiol inhibitor HSCH2CH(CH2Ph)CO-L-Ala-Gly-NH2 and another hydroxamate inhibitor, HONHCOCH(iBu)CO-L-Ala-Gly-NH2, which were both refined to R-values of 0.183/0.198 at 0.240/0.225-nm resolution. The inhibitor thiol and hydroxamate groups ligand the catalytic zinc, giving rise to a slightly distorted tetrahedral and trigonal-bipyramidal coordination sphere, respectively. The thiol inhibitor diastereomer with S-configuration at the P1' residue (corresponding to an L-amino acid analog) binds to HNC. Its peptidyl moiety mimics binding of primed (P1'-P3') residues of the substrate. In combination with our first structure a continuous hexapeptide corresponding to a peptide substrate productively bound to HNC was constructed and energy-minimized. Proteolytic cleavage of this Michaelis complex is probably general base-catalyzed as proposed for thermolysin, i.e. a glutamate assists nucleophilic attack of a water molecule. Although there are many structural and mechanistic similarities to thermolysin, substrate binding to MMPs differs due to the interactions beyond S1'-P1'. While thermolysin binds substrates with a kink at P1', substrates are bound in an extended conformation in the collagenases. This property explains the tolerance of thermolysin for D-amino acid residues at the P1' position, in contrast to the collagenases. The third inhibitor, HONHCOCH(iBu)CO-L-Ala-Gly-NH2, unexpectedly binds in a different manner than anticipated from its design and binding mode in thermolysin. Its hydroxamate group obviously interacts with the catalytic zinc in a favourable bidentate manner, but in contrast its isobutyl (iBu) side chain remains outside of the S1' pocket, presumably due to severe constraints imposed by the adjacent planar hydroxamate group. Instead, the C-terminal Ala-Gly-NH2 tail adopts a bent conformation and inserts into this S1' pocket, presumably in a non-optimized manner. Both the isobutyl side chain and the C-terminal peptide tail could be replaced by other, better fitting groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Targeting dendritic cells to enhance DNA vaccine potency. (2001)

DNA vaccination that can induce both cellular and humoral immune responses has become an attractive immunization strategy against cancer and infection. Dendritic cells (DCs) play a critical role in the induction of immune responses by DNA vaccination. However, a major problem of DNA vaccination is its limited potency, because only a very limited fraction of injected DNA molecules are taken up by DCS: In this study, we describe a novel DNA vaccination strategy to enhance uptake and presentation of antigens by DCS: Specifically, we developed a DNA vaccine based upon expression of a model hepatitis B virus (HBV) e antigen fused to an IgG Fc fragment. After vaccination, the DNA are taken up by cells that produce and secrete the antigen-Fc fusion proteins. The secreted fusion proteins, in addition to inducing B cells, are efficiently captured and processed by DCs via receptor-mediated endocytosis and then presented to the MHC class II and as -I (cross-priming). The results of this study demonstrate that broad enhancement of antigen-specific CD4+ helper, CD8+ cytotoxic T-cell, and B-cell responses can be achieved by this DNA vaccination strategy. Thus, the strategy capable of inducing all arms of the adaptive immunity may provide a novel, generic design for the development of therapeutic and preventive DNA vaccines.