gebhart/patrepeval huggingface dataset:
Multiplex PCR of bcr-abl fusion transcripts in Philadelphia positive acute lymphoblastic leukemia. (1995)

We describe a multiplex PCR assay for the detection of bcr-abl fusion mRNA in Philadelphia chromosome positive acute lymphoblastic leukemia (Ph + ALL). The assay provides a quick method for screening p190 (e1:a2) and p210 (b2:a2 or b3:a2) bcr-abl mRNAs simultaneously. The assay proves to be highly sensitive with detection of as little as one positive bcr-abl-expressing cell in a background of 10(5) negative bcr-abl cells. Bone marrow and peripheral blood specimens from six patients were in total accordance when run by multiplex PCR and by the single primer PCR approach. The multiplex bcr-abl assay may prove to be highly useful for screening newly diagnosed patients with ALL for the bcr-abl fusion transcript and in following the course of disease during therapy.
Molecular Localization of an Ion-Binding Site Within the Pore of Mammalian Sodium Channels (1992)

Sodium channels are the major proteins that underlie excitability in nerve, heart, and skeletal muscle. Chemical reaction rate theory was used to analyze the blockage of single wild-type and mutant sodium channels by cadmium ions. The affinity of cadmium for the native tetrodotoxin (TTX)-resistant cardiac channel was much higher than its affinity for the TTX-sensitive skeletal muscle isoform of the channel (microliters). Mutation of Tyr401 to Cys, the corresponding residue in the cardiac sequence, rendered microliters highly susceptible to cadmium blockage but resistant to TTX. The binding site was localized approximately 20% of the distance down the electrical field, thus defining the position of a critical residue within the sodium channel pore.
Cyclodextrin-Based Polymeric Materials: Synthesis, Properties, and Pharmaceutical/Biomedical Applications (2009)

This review describes the synthesis, properties, and, in particular, biomedical and pharmaceutical applications of an upcoming class of polymeric networks and assemblies based on cyclodextrins (CDs). CDs are cyclic oligosaccharides composed of alpha-1,4-coupled d-glucose units, which contain a hydrophobic internal cavity that can act as a host for various, generally lipophilic, guest molecules. Because of this unique physicochemical property, commonly referred to as inclusion complex formation, CDs have often been used to design polymeric materials, such as hydrogels and nanoparticles. Polymeric systems based on CDs exhibit unique characteristics in terms of mechanical properties, stimuli-responsiveness, and drug release characteristics. In this contribution, first, an outline is given of covalently cross-linked polymeric networks in which CD moieties were structurally incorporated to modulate the network strength as well as the complexation and release of low molecular weight drugs. Second, physically assembled polymeric systems are discussed, of which the formation is accomplished by inclusion complexes between polymer-conjugated CDs and various guest molecule-derivatized polymers. Due to their physical nature, these polymeric systems are sensitive to external stimuli, such as temperature changes, shear forces and the presence of competing CD-binding molecules, which can be exploited to use these systems as injectable, in situ gelling devices. In recent years, many interesting CD-containing polymeric systems have been described in literature. These systems have to be optimized and extensively evaluated in preclinical studies concerning their safety and efficacy, making future clinical applications of these materials in the biomedical and pharmaceutical field feasible.
Transcription Activator-Like Effector Proteins Induce the Expression of the Frataxin Gene (2012)

Genes encoding transcription activator-like effector (TALE) proteins may be engineered to target specific DNA sequences. TALEs fused with a transcription activator can be used to specifically induce the expression of a gene. This could lead to completely new therapies for several diseases. We have applied this potential therapeutic approach to Friedreich ataxia (FRDA), as an example. FRDA is due to reduced expression of frataxin because of elongation of a trinucleotide (GAA) repeat in intron 1. Our aim was to develop a potential treatment for FRDA by increasing the expression of the frataxin gene. We engineered 12 TALE genes (TALE(Frat)) encoding TALE(Frat) proteins, each specifically targeting different 14-bp DNA sequences within the proximal region of the human frataxin promoter. When the genes encoding these TALE(Frat) proteins were fused with a transcription activator, that is, four VP16 peptides (i.e., VP64), the resulting TALE(Frat)-VP64 proteins induced the expression of an mCherry reporter gene fused to a mini-cytomegalovirus promoter able to be activated by the insertion of the frataxin proximal promoter upstream to the minipromoter. These TALE(Frat)-VP64 proteins also increased, by 2- to 3-fold, frataxin gene expression (detected by qRT-PCR) in the cells. We conclude that TALE(Frat) proteins targeting the frataxin promoter may be used to increase the expression of frataxin mRNA and potentially could alleviate the symptoms of Friedreich ataxia. TALE methodology opens a new field of research, which could be used to develop TALE proteins to treat other diseases by inducing the expression of specific genes.
Synthesis of 3-Arylcoumarins, 2-Aroylbenzofurans and 3-Aryl-2H-1,4-benzoazines Under Phase-Transfer Catalysis Conditions (1987)

Abstract The synthesis of 3-arylcoumarins, 2-aroyl-benzofurans and 3-aryl-2H-1,4-benzoxazines under phase transfer catalysis conditions is described.
A note on the maximum flow through a network (1956)

This note discusses the problem of maximizing the rate of flow from one terminal to another, through a network which consists of a number of branches, each of which has a limited capacity. The main result is a theorem: The maximum possible flow from left to right through a network is equal to the minimum value among all simple cut-sets. This theorem is applied to solve a more general problem, in which a number of input nodes and a number of output nodes are used.
Bit-Serial Reed Solomon Decoders in VLSI (1984)

Reed-Solomon codes are known to provide excellent error-correcting capabilities on many types of communication channels. Although efficient decoding algorithms have been known for over fifteen years, currently available decoder systems are large both in size and in power consumption. Such systems typically use a single, very fast, fully parallel finite-field multiplier in a sequential architecture. Thus, more processing time is required as the code redundancy increases. By using many arithmetic units on a single chip, it is possible to exploit the concurrency inherent in the decoding algorithms to attain performance levels previously possible only with large ECL systems. An investigation into the structure of binary extension fields reveals that the common arithmetic operations used in decoding can be implemented quite efficiently in a bit-serial fashion, using any of several bases over GF(2). Berlekamp's dual-basis multiplier is generalized to the product of two arbitrary field elements, and a necessary and sufficient condition is then derived for the existence of a self-dual basis. The efficient methods presented for bit-serial multiplicative inversion greatly reduce the complexity traditionally associated with this operation. Using these bit-serial techniques, several architectures for implementing each phase of the known Reed-Solomon decoding algorithms are presented and compared. Simple methods are presented to allow power-sum syndrome decoders to handle codes with a variety of block lengths and redundancies. Each approach comes within a factor of log n (where n is the block length of the code) of the recently derived asymptotic lower bounds for both time and area. Results from a student project to lay out a prototype decoder chip using the Berlekamp-Massey algorithm are also discussed. By utilizing the parallelism inherent in the key equation solution, these architectures can decode received words a t a speed independent of the redundancy of the code.
Chromosomal aberrations in prostate cancer xenografts detected by comparative genomic hybridization (2002)

A major problem in studying prostate cancer has been the lack of model systems because of the difficulties in growing prostate cancer cells in vitro. Recently, however, several human prostate cancer xenografts, grown in immune-deficient mice, have been established. Here, we characterized 13 such xenografts (LuCaP 23.8, 23.12, 35, 41, 49, 58, 69, 70, 73, LAPC-4AD, LAPC-4AI, LAPC-9AD, and LAPC-9AI) as well as one prostate cancer cell line (22Rv1) derived from a xenograft for chromosomal alterations by comparative genomic hybridization and a modification of multicolor fluorescence in situ hybridization. On average, the xenografts contained 13 (range 5-28) aberrations, 5 (1-13) gains, and 8 (1-15) losses, per case. The chromosome arms that most often contained losses were 2q, 5q, 6q, 8p, 13q, and 18q, and gains were 7q, 8q, and Xq. The same regions were previously shown to be often altered in advanced prostate carcinomas in patients. The androgen-dependent and corresponding androgen-independent sublines of LAPC-4 and LAPC-9 shared all genetic alterations, suggesting that the transition of the growth from androgen dependency to independence does not involve major chromosomal aberrations in these two models. In conclusion, the identified genetic aberrations lay the groundwork for further detailed genetic analyses of these xenografts.
Phase III Study of Afatinib or Cisplatin Plus Pemetrexed in Patients With Metastatic Lung Adenocarcinoma With EGFR Mutations (2023)

Afatinib is associated with prolongation of PFS when compared with standard doublet chemotherapy in patients with advanced lung adenocarcinoma and <i>EGFR</i> mutations.
Trophic Interactions Between Astroglial Cells and Hippocampal Neurons in Culture (1980)

Astroglial cells in primary culture release factors into the medium that promote the growth and prolong the survival of rat hippocampal neurons in vitro.
Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology. (1986)

Dendritic cells (DC) from human peripheral blood, known to adhere transiently and to become non-adherent by 16 hr, can be separated in the low-density interface on hypertonic Metrizamide gradients. Many more low-density cells (5.8% of the mononuclear cells separated on Ficoll) were obtained from the population that was non-adherent after only 90 min. Over 95% of these low-density cells had veiled morphology. A proportion were monocytes by phenotypic and phagocytic properties. One-third of the cells (on average) were DC on the basis of lack of monocyte phenotype and of potency as stimulators in the mixed lymphocyte reaction. Including both the 16 hr and 90 min non-adherent cells, over 2% of the mononuclear cells isolated from human peripheral blood may be DC.
Two stream approximation to radiative transfer equation: An alternative method of solution (2005)

An alternative analytical method of solution to radiative transfer equation in the two-stream approximation is studied. The method is formulated in terms of the diffusion-type equation for radiative transfer associated with the fluxes (irradiances) F d = F + - F - and F s = F + + F - , where F + and F - are defined as the upward and downward fluxes respectively. The diffusion-type equations are independent and therefore the method of solution is algebraically easier and faster than that used to solve the two coupled differential equations associated with F + and F - .
Angiotensin II for the Treatment of Vasodilatory Shock (2017)

Angiotensin II effectively increased blood pressure in patients with vasodilatory shock that did not respond to high doses of conventional vasopressors. (Funded by La Jolla Pharmaceutical Company; ATHOS-3 ClinicalTrials.gov number, NCT02338843 .).
Autocrine cytokine signaling mediates effects of rhinovirus on airway responsiveness (2000)

The airway responses to allergen exposure in allergic asthma are qualitatively similar to those elicited by specific viral respiratory pathogens, most notably rhinovirus (RV), suggesting that the altered airway responsiveness seen in allergic asthma and that elicited by viral respiratory tract infection may share a common underlying mechanism. To the extent that T helper cell type 2 (Th2) cytokines have been implicated in the pathogenesis of allergic asthma, this study examined the potential role(s) of Th2-type cytokines in mediating pro-asthmatic-like changes in airway smooth muscle (ASM) responsiveness after inoculation of naive ASM with human RV. Isolated rabbit ASM tissues and cultured human ASM cells were exposed to RV (serotype 16) for 24 h in the absence and presence of monoclonal blocking antibodies (MAbs) or antagonists directed against either the Th2-type cytokines interleukin (IL)-4 and IL-5, intercellular adhesion molecule (ICAM)-1 (the endogenous host receptor for most RVs), or the pleiotropic proinflammatory cytokine IL-1beta. Relative to control (vehicle-treated) tissues, RV-exposed ASM exhibited significantly enhanced isometric contractility to acetylcholine and impaired relaxation to isoproterenol. These pro-asthmatic-like changes in ASM responsiveness were ablated by pretreating the RV-exposed tissues with either IL-5-receptor-alpha blocking antibody or human recombinant IL-1-receptor antagonist, whereas IL-4 neutralizing antibody had no effect. Extended studies further demonstrated that inoculation of ASM cells with RV elicited 1) an increased mRNA expression and release of IL-5 protein, which was inhibited in the presence of anti-ICAM-1 MAb, and 2) an enhanced release of IL-1beta protein, which was inhibited in the presence of IL-5 receptor-alpha antibody. Collectively, these observations provide new evidence demonstrating that RV-induced changes in ASM responsiveness are largely attributed to ICAM-1-dependent activation of a cooperative autocrine signaling mechanism involving upregulated IL-5-mediated release of IL-1beta by the RV-exposed ASM itself.
Expression of Bovine Cytochrome P450c21 and Its Fused Enzymes with Yeast NADPH-Cytochrome P450 Reductase in Saccharomyces cerevisiae (1990)

Recombinant plasmids for expression of bovine cytochrome P450c21 (pA gamma 2), both P450c21 and yeast NADPH-cytochrome P450 reductase (pAR gamma 1), P450c21/yeast reductase fused enzymes (pAF gamma R1, pAF gamma R2, and pAF gamma R20), and yeast reductase/P450c21 fused enzymes (pAFR gamma 1 and pAFR gamma 2) were constructed by using expression vector pAAH5. The plasmids were each introduced into the yeast Saccharomyces cerevisiae AH22 cells. The recombinant yeast strains AH22/pA gamma 2 (Y21) and AH22/pAR gamma 1 (Y21R) produced 2-3 X 10(3) molecules of P450c21 per cell. The cultures of both strains converted progesterone and 17 alpha-hydroxyprogesterone into 11-deoxycorticosterone and 11-deoxycortisol, respectively. The 21-hydroxylase activity per cell of the strain Y21R was about three times higher than that of the strain Y21, probably due to overproduction of yeast reductase. The recombinant yeast strains AH22/pAF gamma R1 (Y21RF1), AH22/pAF gamma R2 (Y21RF2), and AH22/pAF gamma R20 (Y21RF20) produced about 1.1-2.0 X 10(4) molecules per cell of the corresponding P450c21/yeast reductase fused enzymes. The specific 21-hydroxylase activity toward 17 alpha-hydroxyprogesterone per cell of the strains Y21RF1, Y21RF2, and Y21RF20 was about 21, 28, and 49 times higher than that of the strain Y21, respectively. Thus, the fused enzymes were superior to P450c21 in the specific activity and in the expression level in the yeast. The Km values for 17 alpha-hydroxyprogesterone of P450c21 in the strains Y21 and Y21R, and of the fused enzymes in the strains Y21RF1 and Y21RF2 were 0.29, 0.30, 0.67, and 0.65 microM, respectively. The Vmax values of P450c21 in the strains Y21 and Y21R, and of the fused enzymes in the strains Y21RF1 and Y21RF2 were 28, 124, 151, and 222 moles/min.mole P450c21 or fused enzyme, respectively. These results indicated that the fused enzymes showed lower affinity for the substrate, probably due to structural modification and higher reaction rates through efficient intramolecular electron transfer as compared with those of P450c21. While the strain AH22/pAFR gamma 2 (YR21F2) produced about 3 X 10(4) molecules per cell of the reductase/P450c21 fused enzyme, the specific 21-hydroxylase activity of the fused enzyme toward 17 alpha-hydroxyprogesterone was extremely low, suggesting that the structure of the fused enzyme might not be suited for electron transfer in yeast microsomes.
Dilute and concentrated solution properties of zigzag polymers comprising long rodlike segments with freely rotating joints (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDilute and concentrated solution properties of zigzag polymers comprising long rodlike segments with freely rotating jointsShaul M. AharoniCite this: Macromolecules 1987, 20, 4, 877–884Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April 1987https://pubs.acs.org/doi/10.1021/ma00170a028https://doi.org/10.1021/ma00170a028research-articleACS PublicationsRequest reuse permissionsArticle Views127Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Six Amino Acid Changes Confined to the Leucine-Rich Repeat b-Strand/b-Turn Motif Determine the Difference between the P and P2 Rust Resistance Specificities in Flax (2001)

Peter N. Dodds, Gregory J. Lawrence, Jeffrey G. Ellis, Six Amino Acid Changes Confined to the Leucine-Rich Repeat β-Strand/β-Turn Motif Determine the Difference between the P and P2 Rust Resistance Specificities in Flax, The Plant Cell, Vol. 13, No. 1 (Jan., 2001), pp. 163-178
Medical applications of magnet devices (1975)

The use of magnetic devices in medically-related applications has often been frustrated by insufficient magnetic force, or by an inappropriately designed device. This paper describes magnetic treatment systems which were designed with the benefit of the cooperation of hospital personnel at all levels. Two systems are described in detail. First, a superconducting magnet, with integral orientation system, intended for use in intravascular catheter guidance. The maximum field and gradient produced by this solenoid are 20,000 Oe, and 2250 Oe/cm, respectively. The system is both powerful, and easy to use, by virtue of its completely portable design. The second is a magnetic traction device which has been successfully employed in the treatment of esophageal atresia.
Transition‐Metal‐Catalyzed Benzylation of Arenes and Heteroarenes (2004)

Friedel–Crafts-type benzylation of various aromatic and heteroaromatic compounds with benzylic acetates, alcohols, and carbonates was promoted under mild reaction conditions by a series of Rh, Ir, Pd, and Pt catalysts (see scheme; R1=H, Me; R2=H, Cl, MeO; R3=H, Ac, CO2Me). The desired products were obtained in high yields and with high selectivities.
Amorphous silicon solar cell (1976)

Thin film solar cells, ∼1 μm thick, have been fabricated from amorphous silicon deposited from a glow discharge in silane. The cells were made in a p-i-n structure by using doping gases in the discharge. The best power conversion efficiency to date is 2.4% in AM-1 sunlight. The maximum efficiency of thin-film amorphous silicon solar cells is estimated to be ∼14–15%.
Effects of Chronic Heart Failure on the Capacity of Glucagon to Enhance Contractility and Adenyl Cyclase Activity of Human Papillary Muscles (1971)

Glucagon has a positive inotropic effect in normal hearts but is ineffective in animals with chronic cardiac failure. To assess directly the influence of glucagon on the human myocardium, we measured contractility and activation of adenyl cyclase, the enzyme thought to mediate the inotropic action of glucagon, in left ventricular papillary muscles obtained from 12 patients at mitral valve replacement. On the basis of preoperative ventricular end-diastolic pressures and cardiac output (independent of papillary muscle data) patients were classified in three groups: normal, cardiac failure, and condition indeterminate. Concentration-response curves showed that glucagon caused a mean rise of 11% in peak papillary muscle tension and a rise of 12% in peak rate of tension development in the normal patients; myocardial adenyl cyclase activity from each normal patient rose after glucagon (average, 84%). In the papillary muscles of the patients with cardiac failure, glucagon did not augment either tension or adenyl cyclase activity. In contrast, contractility and adenyl cyclase activity increased after norepinephrine in both normal patients and those with cardiac failure. The indeterminate group had two patients whose papillary muscles responded to glucagon and two whose papillary muscles did not respond. Thus, direct study of human papillary muscles shows that chronic cardiac failure is uniformly associated with complete loss of the normal enhancement of contractility and associated activation of adenyl cyclase after glucagon. This perhaps explains the inefficacy of this drug in treating patients with chronic cardiac failure.
Novel synthesis of purine .beta.-D-nucleosides via purine 8,5'-S-anhydronucleosides (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel synthesis of purine .beta.-D-nucleosides via purine 8,5'-S-anhydronucleosidesYoshihisa Mizuno, Chisato Kaneko, Yuji Oikawa, Takao Ikeda, and Tokuo ItohCite this: J. Am. Chem. Soc. 1972, 94, 13, 4737–4739Publication Date (Print):June 1, 1972Publication History Published online1 May 2002Published inissue 1 June 1972https://pubs.acs.org/doi/10.1021/ja00768a056https://doi.org/10.1021/ja00768a056research-articleACS PublicationsRequest reuse permissionsArticle Views58Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts