gebhart/patrepeval huggingface dataset:
Liposome-mediated delivery of tobacco mosaic virus RNA into tobacco protoplasts: A sensitive assay for monitoring liposome-protoplast interactions (1982)

Tobacco mosaic virus (TMV) RNA was encapsulated in large, unilamellar phospholipid vesicles (liposomes), and the encapsulated TMV RNA was shown to be infectious when incubated with tobacco protoplasts under appropriate conditions. Maximal virus production in protoplasts was observed after their incubation with TMV RNA entrapped in phosphatidylserine/cholesterol liposomes. Infection was dependent on the presence of polyalcohols in the incubation mixture. Other parameters, such as the extent of vesicle binding, the cell-induced leakage of vesicle contents, and the degree of liposome toxicity were shown to be important in determining the efficiency of infectivity. Liposome-mediated delivery offers an efficient and reproducible method for introducing RNA into plant protoplasts.
Forecasting with a Repeat Purchase Diffusion Model (1988)

A methodology for forecasting the sales of an ethical drug as a function of marketing effort before any sales data are available and for updating the forecast with a few periods of sales data is presented. Physicians' perceptions of the drug on a number of attributes, e.g. effectiveness, range of ailments for which appropriate, frequency of prescriptions, are used to estimate the parameters of a model originally proposed by Lilien, Rao and Kalish (Lilien, G. L., A. G. Rao, S. Kalish. 1981. Bayesian estimation and control of detailing effort in a repeat purchase diffusion environment. Management Sci. 27(May) 493–506.). This model conceptualizes the drug adoption process as a repeat purchase diffusion model; sales are expressed as a function of a drug's own and competitive marketing efforts and of word of mouth. The model is first validated in this paper via predictive testing on 19 drugs prescribed by three types of physicians. The forecasting methodology is illustrated using physicians' perceptions on these drugs. Forecasts obtained without any sales data, and updated forecasts using seven periods of sales data are presented, and are encouraging.
The remarkable biology of pollen. (1992)

To reproduce, higher plants utilize a unique multicellular microorganism: the male gametophyte, or pollen grain. The independent lifetime of flowering plant gametophytes is greatly abbreviated compared to that of gametophytes of more primitive plants, yet the angiosperm pollen grain must be able to survive at least briefly free from the sporophytic plant and perform a number of specialized functions before fertilization is accomplished. Although the pollen grain is a rather simple twoor threecelled organism, cytogenetic and mutagenesis experiments indicate that higher plants make a significant investment in genetic material devoted to gametophyte production (Carlson, 1977; Birchler and Schwartz, 1979; Kindiger et al., 1991). Molecular studies identifying antherand pollen-specific genes support the notion that the construction of a functional male gametophyte requires a rather large pool of such genes (Kamalay and Goldberg, 1980; Willing and Mascarenhas, 1984; McCormick, 1991). This is not so surprising given the specialized structures, mechanisms for rapid growth, and cell-cell communication systems during pollen-pistil interactions that have evolved to allow for both the survival of the gametophyte free of the sporophyte and the efficient delivery of sperm to the embryo sac. Studies of certain aspects of the molecular mechanisms of gametophyte development and function have progressed very rapidly. Examples of these include the identification of cis-acting elements determining tissue-specific gene expression in pollen and tapetal cells (Ursin et al., 1989; Koltunow et al., 1990; McCormick et al., 1991) and of mechanisms of self-incompatibility in pollen-pistil interactions (McClure et al., 1989, 1990; Thorsness et al., 1991). The intent of this article is to focus on a few less well studied but intriguing aspects of pollen development and function, with an emphasis on pollen development in maize. For more general reviews of microsporogenesis, the reader is referred to Giles and Prakash (1987) and Mascarenhas (1989). For recent reviews of gene expression during pollen development, see Mascarenhas (1990) and McCormick (1991). OVERVIEW OF POLLEN DEVELOPMENT
A nitric oxide synthase transgene ameliorates muscular dystrophy in mdx mice (2001)

Dystrophin-deficient muscles experience large reductions in expression of nitric oxide synthase (NOS), which suggests that NO deficiency may influence the dystrophic pathology. Because NO can function as an antiinflammatory and cytoprotective molecule, we propose that the loss of NOS from dystrophic muscle exacerbates muscle inflammation and fiber damage by inflammatory cells. Analysis of transgenic mdx mice that were null mutants for dystrophin, but expressed normal levels of NO in muscle, showed that the normalization of NO production caused large reductions in macrophage concentrations in the mdx muscle. Expression of the NOS transgene in mdx muscle also prevented the majority of muscle membrane injury that is detectable in vivo, and resulted in large decreases in serum creatine kinase concentrations. Furthermore, our data show that mdx muscle macrophages are cytolytic at concentrations that occur in dystrophic, NOS-deficient muscle, but are not cytolytic at concentrations that occur in dystrophic mice that express the NOS transgene in muscle. Finally, our data show that antibody depletions of macrophages from mdx mice cause significant reductions in muscle membrane injury. Together, these findings indicate that macrophages promote injury of dystrophin-deficient muscle, and the loss of normal levels of NO production by dystrophic muscle exacerbates inflammation and membrane injury in muscular dystrophy.
Design of a discriminating fingerprint for G-protein-coupled receptors (1993)

A systematic method for designing discriminating protein sequence fingerprints is described. The approach used is iterative, and diagnostic performance is evaluated in terms of the relative abilities of sequences to match with individual elements of the fingerprint. The method allows complete protein folds to be characterized in terms of a number of separate 'features', without the requirement to define specific intervals between them, and is described here with reference to the derivation of a fingerprint for G-protein-coupled receptors: this comprises the seven hydrophobic regions shown by protein chemistry approaches to be membrane-spanning. The fingerprint is potently diagnostic of all sequences of this type in the database in which it was derived (the OWL composite sequence database, version 8.1), and has continued to perform well on subsequent database updates, identifying 240 receptors in OWL17.0. Results are compared with a commonly used pattern template for this class of receptors. The investigation suggests that discriminating power is improved in the fingerprint approach because the recognition of individual features is made mutually conditional. Furthermore, by avoiding the definition of predetermined feature separations, members of protein families possessing all or only part of the fingerprint may be identified.
Vehicular Positioning System (1986)

There is a US Army vehicular positioning program under way called MAFIS (Mobile Automated Field Instrumentation System). Though its use is intended for full scale battlefield operational testing of mobile ground units and aircraft systems, the implications for extending this technology toward commercial aplications are the thrust of this paper.
Secreted proteins from normal and SV40 transformed human MRC-5 fibroblasts: toward establishing a database of human secreted proteins. (1987)

Analysis by means of computerized two-dimensional gel electrophoresis (NEPHGE, IEF) of the [35S]-methionine labeled proteins secreted by normal human MRC-5 fibroblasts revealed 476 polypeptides (258 acidic and 218 basic), many of which appeared as charge trains due to modification. Similar analysis of the proteins secreted by SV40 transformed MRC-5 fibroblasts (MRC-5 V2) showed a striking decrease in the levels of many of these proteins as well as the appearance (or increased synthesis) of 47 polypeptides that were either absent or present in very low amounts in normal cells. Of the major secreted polypeptides whose relative proportion decreased dramatically in the MRC-5 V2 cells, 15 were found to be abundant components of other normal (nontransformed) fibroblasts (W138, Xeroderma pigmentosum cell lines). Low levels of these radioactively labeled polypeptides were observed in transformed human cell lines of fibroblast (W138, SV40, HT1080), epithelial (HeLa, transformed amnion cells (AMA), A431, A459) and myeloid (HL-60) origin. No major secreted polypeptide from MRC-5 V2 cells was synthesized exclusively by the transformed cell lines.
Serial Serum C-Reactive Protein Levels in the Diagnosis of Neonatal Infection (1998)

Serial CRP levels are useful in the diagnostic evaluation of neonates with suspected infection. Two CRP levels <1 mg/dL obtained 24 hours apart, 8 to 48 hours after presentation, indicate that bacterial infection is unlikely. The sensitivity of a normal CRP at the initial evaluation is not sufficient to justify withholding antibiotic therapy. The positive predictive value of elevated CRP levels is low, especially for culture-proven early-onset infections.
Chemotherapeutic agents with an imidazole moiety. III. Synthesis and microbiological activity of new 1,4-diarylimidazole and 1,4-pyrrolimidazolphenylene derivatives. (1992)

New 1,4-diarylimidazole and 1,4-pyrrolimidazol-phenylene derivatives were prepared in attempt to deepen S.A.R. study on chemotherapeutic agents with an imidazole moiety. Antimicrobial data in comparison with antifungal antibiotic pyrrolnitrin showed that all tested compounds are practically inactive against blastomycetes but some derivatives exhibited selective activity against strains of gram-negative bacteria. The results obtained are discussed on the basis of structure-activity relationships.
Novel heterocyclic nitrofurfural hydrazones. In vivo antitrypanosomal activity (1976)

Hydrazine derivatives of several pyrazolo[1,5-alpha]pyrimidines (A), pyrazolo[1,5-alpha]-1,3,5-triazines (B), s-triazolo[1,5-alpha]pyrimidines (C), and imidazo[1,2-alpha]pyrimidines (D) were synthesized and condensed with 5-nitrofurfural in order to obtain the corresponding nitrofurfural hydrazones of each heterocycle (1d-14d). Each compound was screened for in vitro activity against Trypanosoma cruzi. The compounds were then tested in vivo against experimental infections of T. cruzi in laboratory (C3H/He strain) mice. An interesting structure-activity relationship was uncovered, revealing that 5-methyl-7-(5-nitrofurfurylidenehydrazino)pyrazolo[1,5-alpha]pyrimidine (2d) greatly increased the mean survival time (IMST) of mice with terminal infections. Subtle alterations in the structure of 2d, such as the substitution of a 5-hydrogen for the 5-methyl group (1d) or the substitution of the 3-hydrogen by the water-soluble 3-sulfonic acid (3d) or 3-sodium sulfonate (4d), resulted in a drastic loss of in vivo and in vitro activity.
Efficacy of Pneumococcal Vaccination in Adults (1994)

Pneumococcal vaccination appears efficacious in reducing bacteremic pneumococcal pneumonia in low-risk adults. However, evidence from randomized controlled trials fails to demonstrate vaccine efficacy for pneumococcal infection-related or other medical outcomes in the heterogeneous group of subjects currently labeled as high risk.
Broadcast digital video as a low-cost overlay to baseband digital-switched services on a PON (1996)

Summary form only given. Here we show how low-cost lasers can be used to deliver a broad selection of broadcast digital signals to multiple optical network units (ONU), for low cost. In addition, these signals are transmitted as a video overlay, using subcarrier multiplexing, with broadband digital-switched services.
Erratum to: ‘DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs’ (2016)

5'-phosphorilated oligonucleotides (IDT)".
Effects of pH and Buffer Concentration on the Thermal Stability of Etanercept Using DSC and DLS (2014)

The protein size, electrical interaction, and conformational stability of etanercept (marketed as Enbrel®) were examined by thermodynamic and light scattering methods with changing pH and buffer concentration. As pH of etanercept increased from pH 6.6 to 8.6, electrical repulsion in the solution increased, inducing a decrease in protein size. However, the size changed less in high buffer concentration and irreversible aggregation issues were not observed; in contrast, aggregates of about 1000 nm were observed in low buffer concentration at the pH range. Three significant unfolding transitions (Tm) were observed by differential scanning calorimetry (DSC). Unlikely to Tm1, Tm2 and Tm3 were increased as the pH increased. Higher Tm at high buffer concentration was observed, indicating increased conformational stability. The apparent activation energy of unfolding was further investigated since continuous increase of Tm2 and Tm3 was not sufficient to determine optimal conditions. A higher energy barrier was calculated at Tm2 than at Tm3. In addition, the energy barriers were the highest at pH from 7.4 to 7.8 where higher Tm1 was also observed. Therefore, the conformational stability of protein solution significantly changed with pH dependent steric repulsion of neighboring protein molecules. An optimized pH range was obtained that satisfied the stability of all three domains. Electrostatic circumstances and structural interactions resulted in irreversible aggregation at low buffer concentrations and were suppressed by increasing the concentration. Therefore, increased buffer concentration is recommended during protein formulation development, even in the earlier stages of investigation, to avoid protein instability issues.
A Rapid Flp-In System for Expression of Secreted H5N1 Influenza Hemagglutinin Vaccine Immunogen in Mammalian Cells (2011)

Our data suggest that the 293 Flp-In system could serve as a platform for rapid expression of HA immunogens in mammalian cells from emerging influenza strains.
Cellular response to conditional expression of hepatitis C virus core protein in Huh7 cultured human hepatoma cells (2002)

Data suggesting that the hepatitis C virus (HCV) core protein influences normal cellular processes remain controversial. To determine the effects of core on cellular gene expression in hepatocytes, we developed a human hepatoma (Huh7)-derived cell line with tightly regulated core expression under the control of a tetracycline-regulated promoter. Cells expressing core did not have impaired proliferative abilities. Changes in gene expression profiles in response to core expression were determined using commercial oligonucleotide microarrays (Affymetrix GeneChip). Significant increases were observed in the abundance of mRNA-encoding members of the metallothionein (MT) family, as well as nicotinamide N-methyltransferase (NNMT) and glutathione peroxidase-like protein (GPLP). These changes did not result from removal of tetracycline from growth media, and were confirmed in reverse-transcription polymerase chain reaction (RT-PCR) assays. They suggest that core protein expression leads to intracellular oxidative stress, and that vital cellular functions are, in turn, protected by up-regulation of cellular antioxidant defense mechanisms. In conclusion, these findings can explain many potentially conflicting prior observations concerning the effects of core on cellular physiology, and are of relevance to the role of core protein in the pathogenesis of HCV-related fibrosis and hepatocellular carcinoma.
Microfabricated preconcentrator-focuser for a microscale gas chromatograph (2003)

The design, fabrication, and testing of a preconcentrator-focuser (PCF), consisting of a thick micromachined Si heater packed with a small quantity of a granular adsorbent material are described. The PCF is developed to capture and concentrate vapors for subsequent focused thermal desorption and analysis in a micro gas chromatograph. The microheater contains an array of high-aspect-ratio, etched-Si heating elements, 520 μm (h)×50 μm (w)×3000 μm (l), bounded by an annulus of Si and thermally isolated from the remaining substrate by an air gap. This structure is sandwiched between Pyrex glass plates with inlet/outlet ports that accept capillary tubes for sample flow and is sealed by anodic bonding (bottom) and rapidly annealed glass/metal/Si solder bonding (top). The large microheater surface area allows for high adsorption capacity and efficient, uniform thermal desorption of vapors captured on the adsorbent within the structure. The adsorbent consists of roughly spherical granules, ∼200 μm in diameter, of a high-surface-area, graphitized carbon. Key design considerations, fabrication technologies, and results of performance tests are presented with an emphasis on the thermal desorption characteristics of several representative volatile organic compounds as a function of volumetric flow rates and heating rates. Preconcentration factors as high as 5600 and desorbed peak widths as narrow as 0.8 s are achieved from 0.25-L samples of benzene at modest heating rates. The effects of operating variables on sensitivity, chromatographic resolution, and detection limits are assessed. Testing of this PCF with a micromachined separation column and integrated sensor array is discussed briefly.
Functional heterogeneity among CD4+ encephalitogenic T cells in recruitment of CD8+ T cells in experimental autoimmune encephalomyelitis. (1989)

Inoculation of Lewis rats with live or attenuated (irradiated or paraformaldehyde-fixed) CD4+ encephalitogenic T cells (S1 line) protects the recipients from transferred experimental autoimmune encephalomyelitis (tEAE) induced by S1 cells. A CD8+ T lymphocyte population specifically activated against the EAE-inducing S1 cells can be readily isolated from the lymphoid organs of pretreated animals. We show, in the present study, that encephalitogenic T cell lines derived from Lewis rats differ in their ability to induce resistance against tEAE in vivo and to stimulate CD8+ cell proliferation in vitro. We also demonstrate that the S19 line of encephalitogenic T cells, in combination with myelin basic protein (MBP), can stimulate CD8+ cell proliferation in vitro. The CD8+ cells generated in this way strongly suppress MBP-specific T cell proliferation in vitro. This combined effect of T cells and MBP was also evident in vivo. Neither S19 cells nor MBP alone induced resistance against S19-mediated tEAE, rather coinjection of these cells and MBP was required. Our results suggest that resistance to EAE is mediated by distinct populations of encephalitogenic T cells that activate Ts cells through different mechanisms. In some instances, both autoreactive T cells and their relevant autoantigen(s) may be needed to activate Ts cells in vivo.
Programmable demultiplexer/demodulator processor (2002)

Low to medium bit rate digital transmission formats are increasingly being used for satellite communications to reduce the size and cost of earth stations. A combined demultiplexer and demodulator using digital signal processing is described which uses frequency domain filtering to separate the signals and a shared structure for the demodulation process. A special-purpose, high-speed fast Fourier transform (FFT) processor is designed which operates on a 20-MHz bandwidth that contains carriers ranging in bit rate from approximately 1.5 to 11.3 Mb/s. An interpolating filter is used between the demultiplexer and the demodulator to provide the two samples per symbol required for the demodulation process. The major components of the demultiplexer/demodulator are described. The implementation concentrated on designs using low-power complementary metal-oxide semiconductor (CMOS) circuitry. In addition, a testbed was designed and fabricated to provide the multicarrier inputs needed to demonstrates system capability.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Polytopic ligand directed self-assembly—polymetallic [n×n] grids versus non-grid oligomers (2009)

This critical review surveys factors involved in the convergent self-assembly of square [n x n] (n = 2-5) polymetallic grids, using ligands with encoded coordination information appropriate to the metal ion's coordination algorithm. While entropic factors drive these reactions, other effects, including metal ion CFSE, ligand conformational character, electrostatic factors etc., can lead preferentially to non-grid oligomers. It will be of interest to the general supramolecular community, particularly coordination chemists attempting to create predetermined architectures, with applications oriented molecular based properties (86 references).
Tolerance and Alloreactivity of the Ly49D Subset of Murine NK Cells (1999)

Class I-specific stimulatory and inhibitory receptors expressed by NK cell subsets contribute to the alloreactive potential of the self-tolerant murine NK cell repertoire. In this report, we have studied potential mechanisms of tolerance to the function of the positive signaling Ly49D receptor in mice that express one of its ligands, H2-Dd. Our results demonstrate that H2-Dd-expressing mice possess a large Ly49D+ subset of NK cells that is functionally capable of rejecting bone marrow cell (BMC) allografts in vivo and lysing allogeneic Con A lymphoblasts in vitro. Also, we show that the Ly49D receptor is responsible for the ability of H2b/d F1 hybrid mice to reject H2d/d parental BMC (hybrid resistance). Thus, deletion or anergy of Ly49D+ cells in H2-Dd+ hosts cannot explain self tolerance. Our functional studies revealed that coexpression of the Dd-specific Ly49A or Ly49G2 inhibitory receptors by Ly49D+ cells resulted in tolerance to Dd+ targets, while coexpression of Kb-specific inhibitory receptors Ly49C/I resulted in tolerance to Kb+ targets. Only in H2d/d cells did Ly49C/I dominantly inhibit Ly49D-Dd stimulation. This correlated with an increased mean fluorescence intensity of Ly49C expression, as well as an increased percentage of Ly49C+ cells in the Ly49D+A/G2- compartment. Therefore, we conclude that self tolerance of the Ly49D subset can be achieved through coexpression of a sufficient level of self-specific inhibitory receptors.
Functional Evidence for an RNA Template in Telomerase (1990)

The RNA moiety of the ribonucleoprotein enzyme telomerase from the ciliate Euplotes crassus was identified and its gene was sequenced. Functional analysis, in which oligonucleotides complementary to portions of the telomerase RNA were tested for their ability to prime telomerase in vitro, showed that the sequence 5' CAAAACCCCAAA 3' in this RNA is the template for synthesis of telomeric TTTTGGGG repeats by the Euplotes telomerase. The data provide a direct demonstration of a template function for a telomerase RNA and demarcate the outer boundaries of the telomeric template. Telomerase can now be defined as a specialized reverse transcriptase.
Effect of food on the pharmacokinetics and bioavailability of oral imiquimod relative to a subcutaneous dose (2000)

This study suggests that food does not have a major effect on the rate, extent of absorption or bioavailability of oral imiquimod, and thus, it is suitable to administer imiquimod orally in either the fasted or non-fasted states.
Autonomic mechanism for initiation of rapid firing from atria and pulmonary veins: evidence by ablation of ganglionated plexi (2009)

HFS applied to local atrial and PV sites initiated rapid firing via activation of the interactive autonomic network in the heart. GP in either left side or right side contributes to the rapid firings and AF originating from ipsolateral and contralateral PVs and atrium. Autonomic denervation suppresses or eliminates those rapid firings.