gebhart/patrepeval huggingface dataset:
Porous-coated titanium implant impregnated with a biodegradable protein delivery system (1997)

Tissue ingrowth into porous-coated orthopedic and dental implants is commonly used as a means to achieve long-term fixation of these prostheses. However, the degree of tissue ingrowth is often inadequate and inconsistent. If the pores of these implants are impregnated with a controlled drug release system delivering relevant growth factors, then it might be possible to stimulate more tissue ingrowth. The present study introduces such a system based on biodegradable polymers and investigates its protein release profile and polymer degradation characteristics. Porous coated titanium implants were impregnated with a mixture of a 50%-50% polylactic acid-polyglycolic acid copolymer and a model protein, soybean trypsin inhibitor. Control implants contained only the polymer and no protein. The implants were subjected to hydrolytic degradation in phosphate buffered saline at 37 degrees C for periods of 3, 6, and 11 weeks. The protein release and the mass and molecular weight of the polymer were monitored. The results indicate that the protein is released in three distinct phases and the polymer loses almost all its mass and molecular weight by 11 weeks. There was a significant difference in the polymer degradation characteristics between the control and test implants, which might be the result of some complex polymer-protein interactions.
Mice and humans perceive multiharmonic communication sounds in the same way (2001)

Vowels and voiced consonants of human speech and most mammalian vocalizations consist of harmonically structured sounds. The frequency contours of formants in the sounds determine their spectral shape and timbre and carry, in human speech, important phonetic and prosodic information to be communicated. Steady-state partitions of vowels are discriminated and identified mainly on the basis of harmonics or formants having been resolved by the critical-band filters of the auditory system and then grouped together. Speech-analog processing and perception of vowel-like communication sounds in mammalian vocal repertoires has not been demonstrated so far. Here, we synthesize 11 call models and a tape loop with natural wriggling calls of mouse pups and show that house mice perceive this communication call in the same way as we perceive speech vowels: they need the presence of a minimum number of formants (three formants-in this case, at 3.8 + 7.6 + 11.4 kHz), they resolve formants by the critical-band mechanism, group formants together for call identification, perceive the formant structure rather continuously, may detect the missing fundamental of a harmonic complex, and all of these occur in a natural communication situation without any training or behavioral constraints. Thus, wriggling-call perception in mice is comparable with unconditioned vowel discrimination and perception in prelinguistic human infants and points to evolutionary old rules of handling speech sounds in the human auditory system up to the perceptual level.
Development of SU5416, a selective small molecule inhibitor of VEGF receptor tyrosine kinase activity, as an anti-angiogenesis agent. (2000)

Angiogenesis, or the sprouting of new blood vessels, is a central process in the growth of solid tumors. For many cancers, the extent of vascularization of a tumor is a negative prognostic indicator signifying aggressive disease and increased potential for metastasis. Recent efforts to understand the molecular basis of tumor-associated angiogenesis have identified several potential therapeutic targets, including the receptor tyrosine kinases for the angiogenic factor vascular endothelial growth factor (VEGF). Here we review the approach taken at SUGEN, Inc. to discover and develop small molecule inhibitors of receptor tyrosine kinases as anti-angiogenic agents. We focus on SU5416, a selective inhibitor of VEGF receptors that is currently in clinical development for the treatment of advanced malignancies. Its biochemical, biological and pharmacological properties are reviewed and clinical implications discussed.
An Overview of Horizontal Well Completion Technology (1988)

ABSTRACT Highly deviated or horizontal wells have led to new thinking in the application of well completion and stimulation technology. Horizontal drilling technology has advanced rapidly over the past few years. It is now common to be able to drill and control horizontal holes of greater than 1,000ft. displacement. However, horizontal completion technology is still on a steep learning curve and many completion practices need to be refined to contribute to the economic success of a horizontal project. In addition, a better understanding of reservoir performance with horizontal completions will be needed. Proven completion techniques used in less deviated wells have not proved to be cost effective or efficient in this new application. This paper addresses several of these problem areas by discussing the shortcomings of vertical well completion technology and offers solutions to horizontal applications. Specific completion areas discussed include production string isolation, influence of horizontal wellbore deviation, stimulation considerations, sand control and downhole production equipment. Case histories of three Bima Field wells, offshore Indonesia, are presented to illustrate various completion practices.
Tau gene mutations and neurodegeneration (2001)

Abundant neurofibrillary lesions made of the microtubule-associated protein tau constitute a defining neuropathological characteristic of Alzheimer's disease. Filamentous tau protein deposits are also the defining neuropathological characteristic of other neurodegenerative diseases, many of which are frontotemporal dementias or movement disorders, such as Pick's disease, progressive supranuclear palsy and corticobasal degeneration. It is well established that the distribution of tau pathology correlates with the presence of symptoms of disease. However, until recently, there was no genetic evidence linking dysfunction of tau protein to neurodegeneration and dementia. This has now changed with the discovery of close to 20 mutations in the tau gene in frontotemporal dementia with Parkinsonism linked to chromosome 17. All cases with tau mutations examined to date have shown an abundant filamentous tau pathology in brain cells. Pathological heterogeneity is determined to a large extent by the location of mutations in tau. Known mutations are either coding region or intronic mutations located close to the splice-donor site of the intron downstream of exon 10. Most coding region mutations produce a reduced ability of tau to interact with microtubules. Several of these mutations also promote sulphated glycosaminoglycan-induced assembly of tau into filaments. Intronic mutations and some coding region mutations produce increased splicing in of exon 10, resulting in an overexpression of four-repeat tau isoforms. Thus a normal ratio of three-repeat to four-repeat tau isoforms is essential for preventing the development of tau pathology. The new work has shown that dysfunction of tau protein can cause neurodegeneration and dementia.
Development of a symptom distress scale (1978)

Symptom distress was defined as the degree of discomfort reported by the patient in relation to his/her perception of the symptoms being experienced. The ten symptoms included in the symptom distress scale were nausea, mood, appetite, insomnia, pain, mobility, fatigue, bowel pattern, concentration and appearance. The scale was tested on fifty-three patients with advanced medical conditions. Total symptom distress scores for these patients ranged from ten to forty-one; the mean was twenty. The reliability coefficient alpha was .82143 and the standardized alpha was .82557. More testing of the scale is needed, especially with different types of chronic diseases and visible signs of illness.
GABRIEL: a design environment for programmable DSPs (1989)

Gabriel is a retargetable software system for the development of assembly code and microcode for single or multiple programmable DSPs. It is intended to ease code development even for processors that are not easy targets for conventional compilers. Code generation for the Motorola DSP56001 is emphasized. A Thor-based simulator supplies a variety of target multi-DSP architectures based on the DSP56001. The top-level algorithm description is a large grain data flow graph, and a graphical interface using OCT and VEM provides a natural representation of the high level structure of the algorithm.
Substrate bias effects in high-aspect-ratio SiO2 contact etching using an inductively coupled plasma reactor (1995)

High-aspect-ratio sub-half-micron contacts were etched in SiO2 using a high-density C2F6 plasma generated by an inductively coupled, low-pressure reactor. As expected, oxide and resist etch rates increased with increasing bias. The aspect ratio (depth/width) dependence of the etch process was also shown to vary with bias power; at high bias power conditions, reactive ion etching (RIE) lag was observed, where high-aspect-ratio features etch slower than low-aspect-ratio features, and at low bias powers, inverse RIE lag was observed. Increasingly vertical sidewall angles and increasing microtrenching depth were also observed for higher rf bias conditions on contacts, trenches, and steps. Topography modeling shows that the microtrench profiles are consistent with specular ion reflection from sidewalls and the increase in their depths with rf bias is consistent with the energy-dependent etching yields in high-density, fluorocarbon plasma etch chemistries reported by Oehrlein et al. [J. Vac Sci. Technol. A 12, 323, 333 (1994)].
Intravenous Remifentanil (1998)

Remifentanil crosses the placenta but appears to be rapidly metabolized, redistributed, or both. Maternal sedation and respiratory changes occur, but without adverse neonatal or maternal effects.
Determination of Activation and Deactivation Rate Constants of Model Compounds in Atom Transfer Radical Polymerization (2001)

The activation and deactivation rate constants in atom transfer radical polymerization (ATRP) were measured using model compounds. The activation rate constants were determined using HPLC or GC under the kinetic isolation condition achieved by trapping the generated radical with 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO). The deactivation rate constants were measured by trapping 1-phenylethyl radicals with TEMPO in a competitive reaction. The effects of several parameters in ATRP systems were examined, including alkyl groups, ligands, transferred groups, and solvents. The data obtained were consistent with ATRP kinetics and provided further quantitative insights into understanding the ATRP processes.
A gut-brain neural circuit for nutrient sensory transduction (2018)

The brain is thought to sense gut stimuli only via the passive release of hormones. This is because no connection has been described between the vagus and the putative gut epithelial sensor cell-the enteroendocrine cell. However, these electrically excitable cells contain several features of epithelial transducers. Using a mouse model, we found that enteroendocrine cells synapse with vagal neurons to transduce gut luminal signals in milliseconds by using glutamate as a neurotransmitter. These synaptically connected enteroendocrine cells are referred to henceforth as neuropod cells. The neuroepithelial circuit they form connects the intestinal lumen to the brainstem in one synapse, opening a physical conduit for the brain to sense gut stimuli with the temporal precision and topographical resolution of a synapse.
Rapid Color Grading for Fruit Quality Evaluation Using Direct Color Mapping (2010)

Color grading is a crucial step in the processing of fruits and vegetables that directly affects profitability, because the quality of agricultural products is often associated with their color. Most existing automatic color grading systems determine color quality either by directly comparing product color against a predefined and fixed set of reference colors or by using a set of color separating parameters, often in three-dimensional color spaces. Using these methods, it is not convenient for the user to adjust color preferences or grading parameters. In this paper, we present an effective and user-friendly color mapping concept for automated color grading that is well suited for commercial production. User friendliness is often viewed by the industry as a very important factor to the acceptance and success of automation equipment. This color mapping method uses preselected colors of interest specific to a given application to calculate a unique set of coefficients for color space conversion. The three-dimensional RGB color space is converted into a small set of color indices unique to the application. In contrast with more complex color grading techniques, the proposed method makes it easy for a human operator to specify and adjust color-preference settings Tomato and date maturity evaluation and date surface defect detection are used to demonstrate the performance of this novel color mapping concept.
Immune regulatory activity of CD34+ progenitor cells: evidence for a deletion-based mechanism mediated by TNF-α (2004)

Previous studies suggest that cells within the CD34(+) hematopoietic stem cell compartment are endowed with immune regulatory activity. Furthermore, it is possible to expand the human regulatory cells upon short-term culture of purified CD34+ cells with an early-acting cytokine cocktail. We now show that addition of anti-CD28, anti-CD2, interleukin-2 (IL-2), anti-IL-10, or IL-12 to the bulk mixed lymphocyte reaction (MLR) cannot reverse the inhibitory activity of the CD34+ cells, ruling out anergy-based mechanisms or mechanisms involving Th1-Th2 skewing. Furthermore, phenotyping of cells present after addition of CD34+ cells to the bulk MLR ruled out potential induction of plasmacytoid dendritic precursors, known to be endowed with regulatory activity. In contrast, the inhibitory activity of CD34+ cells could be reversed by adding the caspase inhibitor BD-FMK to the bulk MLR, indicating a deletion-based mechanism. The deletion can be inhibited by anti-tumor necrosis factor-alpha (anti-TNF-alpha) and not by anti-transforming growth factor-beta (anti-TGF-beta), suggesting a potential role for TNF-alpha in the regulatory activity of CD34+ cells.
Importance of anchor genomes for any plant genome project (1998)

Progress in agricultural and environmental technologies is hampered by a slower rate of gene discovery in plants than animals. The vast pool of genes in plants, however, will be an important resource for insertion of genes, via biotechnological procedures, into an array of plants, generating unique germ plasms not achievable by conventional breeding. It just became clear that genomes of grasses have evolved in a manner analogous to Lego blocks. Large chromosome segments have been reshuffled and stuffer pieces added between genes. Although some genomes have become very large, the genome with the fewest stuffer pieces, the rice genome, is the Rosetta Stone of all the bigger grass genomes. This means that sequencing the rice genome as anchor genome of the grasses will provide instantaneous access to the same genes in the same relative physical position in other grasses (e.g., corn and wheat), without the need to sequence each of these genomes independently. (i) The sequencing of the entire genome of rice as anchor genome for the grasses will accelerate plant gene discovery in many important crops (e.g., corn, wheat, and rice) by several orders of magnitudes and reduce research and development costs for government and industry at a faster pace. (ii) Costs for sequencing entire genomes have come down significantly. Because of its size, rice is only 12% of the human or the corn genome, and technology improvements by the human genome project are completely transferable, translating in another 50% reduction of the costs. (iii) The physical mapping of the rice genome by a group of Japanese researchers provides a jump start for sequencing the genome and forming an international consortium. Otherwise, other countries would do it alone and own proprietary positions.
Effects of phosphatidylserine in age‐associated memory impairment (1991)

We treated 149 patients meeting criteria for age-associated memory impairment (AAMI) for 12 weeks with a formulation of phosphatidylserine (100 mg BC-PS tid) or placebo. Patients treated with the drug improved relative to those treated with placebo on performance tests related to learning and memory tasks of daily life. Analysis of clinical subgroups suggested that persons within the sample who performed at a relatively low level prior to treatment were most likely to respond to BC-PS. Within this subgroup, there was improvement on both computerized and standard neuropsychological performance tests, and also on clinical global ratings of improvement. The results suggest that the compound may be a promising candidate for treating memory loss in later life.
Projected index computed tomography (2003)

Projected index computed tomography (PICT) is a new imaging technique that provides a computed reconstruction of the index of refraction of a sample. PICT makes use of data from standard optical coherence tomography images taken from several view angles to determine a mapping of the refractive indices of the sample. A rectilinear propagation model is assumed, so the data are understood to be related to the line integral of the refractive index in the beam paths. These data thus provide a set of angular projections of the sample. The spatial distribution of the index of the object may then be reconstructed by use of standard filtered backprojection techniques. The resultant PICT images are free of the spatial distortion that is inherent in standard optical cross-sectional images and correspond well to the manufactured dimensions of specific samples.
Molecular Genetics of Plant Sterol Backbone Synthesis (2006)

Sterols, which are biosynthesized via the cytoplasmic mevalonate (MVA) pathway, are important structural components of the plasma membrane and precursors of steroid hormones in both vertebrates and plants. Ergosterol and cholesterol are the major sterols in yeast and vertebrates, respectively. In contrast, plants produce a wide variety of phytosterols, which have various functions in plant development. Although the general biosynthetic pathway to plant sterols has been defined, the details of the biochemical, physiological, and developmental functions of genes involved in the biosynthetic network and their regulation are not well understood. Molecular genetic analyses are an effective approach to use when studying these fascinating problems. Since three enzymes, 3-hydroxy-3-methylglutaryl CoA reductase, farnesyl diphosphate synthase, and lanosterol synthase, have been functionally characterized in planta, we reviewed recent progress on these enzymes. Arabidopsis T-DNA and transposon insertion mutants are now widely available. The use of molecular genetics, molecular biology, and bioorganic chemical approaches on these mutants, as well as inhibitors of the MVA pathway, should help us to understand plant sterol biosynthesis comprehensively.
Abstract 9624: Suppressive Effects of Irbesartan on Inflammation and Apoptosis in Atherosclerotic Plaques of Apoe-/- Mice: Molecular Imaging with 14C-FDG and 99mTc-annexin A5 (2011)

Background: Although irbesartan, an angiotensin II subtype 1 receptor blocker, reportedly can suppress the progression of atherosclerosis, the mechanisms of the therapeutic effects remain unclear. ...
Reactivity of human Lyme borreliosis sera with a 39-kilodalton antigen specific to Borrelia burgdorferi (1990)

Borrelia burgdorferi is the causative agent of Lyme borreliosis, a spirochetal illness with a variety of acute clinical manifestations that may lead to debilitating neurological and arthritic complications. Diagnosis is difficult because symptoms mimic a variety of unrelated clinical conditions, spirochetes cannot always be isolated from infected patients, and current serological tests are frequently inconclusive because of the presence of cross-reacting non-B. burgdorferi antibodies. To identify antigens specific to B. burgdorferi that could be used in the serodiagnosis of Lyme borreliosis, we screened a Borrelia DNA expression library in Escherichia coli for antigens reactive with human Lyme borreliosis sera. One clone carried a 6.3-kilobase EcoRI chromosomal fragment (pSPR33), which encoded two species-specific antigens with molecular masses of 28 (P28) and 39 (P39) kilodaltons (kDa). These two antigens were immunologically distinct from OspA, OspB, and the 41-kDa flagellin. Ninety-four serum specimens from patients having Lyme borreliosis were tested for reactivity with P39. All of 33 the serum specimens with immunofluorescence assay titers of greater than or equal to 1:256, 13 of 17 serum specimens with titers of 1:128, and 14 of 44 serum specimens with titers of less than or equal to 1:64 reacted with P39. Notably, many sera reactive to P39 did not appear to react with the 41-kDa flagellin. Therefore, antibody to P39 could be mistaken for antibody to the 41-kDa flagellin in tests of human sera by Western blot (immunoblot). Twenty-five control serum specimens, which included sera from syphilitic, relapsing fever, and amyotrophic lateral sclerosis patients as well as from 10 normal individuals, did not react to P39. Our data suggest that P39 may be a useful antigen for the serological confirmation of Lyme borreliosis.
The hypercycle. Coupling of RNA and protein biosynthesis in the infection cycle of an RNA bacteriophage (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe hypercycle. Coupling of RNA and protein biosynthesis in the infection cycle of an RNA bacteriophageManfred Eigen, Christof K. Biebricher, Michael Gebinoga, and William C. GardinerCite this: Biochemistry 1991, 30, 46, 11005–11018Publication Date (Print):November 19, 1991Publication History Published online1 May 2002Published inissue 19 November 1991https://pubs.acs.org/doi/10.1021/bi00110a001https://doi.org/10.1021/bi00110a001research-articleACS PublicationsRequest reuse permissionsArticle Views538Altmetric-Citations99LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
STARD 2015: an updated list of essential items for reporting diagnostic accuracy studies (2015)

Incomplete reporting has been identified as a major source of avoidable waste in biomedical research. Essential information is often not provided in study reports, impeding the identification, critical appraisal, and replication of studies. To improve the quality of reporting of diagnostic accuracy studies, the Standards for Reporting Diagnostic Accuracy (STARD) statement was developed. Here we present STARD 2015, an updated list of 30 essential items that should be included in every report of a diagnostic accuracy study. This update incorporates recent evidence about sources of bias and variability in diagnostic accuracy and is intended to facilitate the use of STARD. As such, STARD 2015 may help to improve completeness and transparency in reporting of diagnostic accuracy studies.