gebhart/patrepeval huggingface dataset:
Increased Gene Transfer into Human Cord Blood Cells by Centrifugation-Enhanced Transduction in Fibronectin Fragment-Coated Tubes (1999)

We investigated whether transduction of human cord blood progenitor cells can be increased by spinoculation in fibronectin fragment CH-296 (FN)-coated tubes. Bicistronic vectors PA317/LgEIN, containing the enhanced green fluorescent protein (EGFP) and neomycin phosphotransferase (neo) genes, and PG13/LgDIN, containing the dihydrofolate reductase and neo genes, were used to transduce CD34-enriched human cord blood cells. Transduction by spinoculation in FN-coated tubes (spin/FN+) was compared with spinoculation in noncoated tubes (spin/FN-) and transduction in plates coated with FN (plate/FN+). Antibody to TGF-beta was added to spin/FN+ to evaluate its impact on transduction. Using producer cell line PA317/LgEIN for transduction of CD34+ cord blood cells, FACS analysis for expression of EGFP revealed mean transduction of 30.6+/-4.3, 9.1+/-1.6, and 21.1+/-6.5% of CD34+ cells in the spin/FN+, spin/FN-, and plate/FN+ arms, respectively. Transduction of CD+CD38low cells was also higher in the spin/FN+ arm as compared with transduction in the spin/FN- arm. These results were corroborated by colony-forming assays. Antibody to TGF-beta did not further increase transduction. Using a different producer cell line, PG13/pLgDIN, a higher number of G418-resistant CFU-GM was observed in the spin/FN+ as compared with the plate/FN+ and spin/FN-arms. NOD/SCID mice were transplanted with transduced, CD34-enriched human cord blood cells, and persistence of transduced human cells was analyzed in the mice marrows after 6-8 weeks: 32.8, 6.0, and 23.9% human G418-resistant CFU-GM colonies were observed in the spin/FN+, spin/FN-, and plate/FN+ arms, respectively. These results suggest that spinoculation in FN-coated tubes increases transduction of early human cord blood progenitor cells as compared with spinoculation in noncoated tubes.
Detection of Foodborne Pathogens by Surface Enhanced Raman Spectroscopy (2018)

Food safety has become an important public health issue in both developed and developing countries. However, as the foodborne illnesses caused by the pollution of foodborne pathogens occurred frequently, which seriously endangered the safety and health of human beings. More importantly, the traditional techniques, such as PCR and enzyme-linked immunosorbent assay, are accurate and effective, but their pretreatments are complex and time-consuming. Therefore, how to detect foodborne pathogens quickly and sensitively has become the key to control food safety. Because of its sensitivity, rapidity, and non-destructive damage to the sample, the surface enhanced Raman scattering (SERS) is considered to be a powerful testing technology that is widely used to different fields. This review aims to give a systematic and comprehensive understanding of SERS for rapid detection of pathogen bacteria. First, the related concepts of SERS are stated, such as its work principal, active substrate, and biochemical origins of the detection of bacteria by SERS. Then the latest progress and applications in food safety, from detection and characterization of targets in label-free method to label method, is summarized. The advantages and limitations of different SERS substrates and methods are discussed. Finally, there are still several hurdles for the further development of SERS techniques into real-world applications. This review comes up with the perspectives on the future trends of the SERS technique in the field of foodborne pathogens detection and some problems to be solved urgently. Therefore, the purpose is mainly to understand the detection of foodborne pathogens and to make further emphasis on the importance of SERS techniques.
Inhibition of cyclin D-CDK4/CDK6 activity is associated with an E2F-mediated induction of cyclin kinase inhibitor activity. (1996)

Alterations of various components of the cell cycle regulatory machinery that controls the progression of cells from a quiescent to a growing state contribute to the development of many human cancers. Such alterations include the deregulated expression of G1 cyclins, the loss of function of activities such as those of protein p16INK4a that control G1 cyclin-dependent kinase activity, and the loss of function of the retinoblastoma protein (RB), which is normally regulated by the G1 cyclin-dependent kinases. Various studies have revealed an inverse relationship in the expression of p16INK4a protein and the presence of functional RB in many cell lines. In this study we show that p16INK4a is expressed in cervical cancer cell lines in which the RB gene, Rb, is not functional, either as a consequence of Rb mutation or expression of the human papillomavirus E7 protein. We also demonstrate that p16INK4a levels are increased in primary cells in which RB has been inactivated by DNA tumor virus proteins. Given the role of RB in controlling E2F transcription factor activity, we investigated the role of E2F in controlling p16INK4a expression. We found that E2F1 overexpression leads to an inhibition of cyclin D1-dependent kinase activity and induces the expression of a p16-related transcript. We conclude that the accumulation of G1 cyclin-dependent kinase activity during normal G1 progression leads to E2F accumulation through the inactivation of RB, and that this then leads to the induction of cyclin kinase inhibitor activity and a shutdown of G1 kinase activity.
The effect of dietary inclusion of yeast culture (Yea-Sacc) on patterns of rumen fermentation, food intake and growth of intensively fed bulls (1992)

Abstract The effects of a barley beef diet without (control) and with a yeast culture (YC) on rumen fermentation, in vivo diet digestibility, nitrogen retention, live-weight gain and food intake were evaluated using 13 Limousin × British Friesian bulls per treatment. The YC was composed of the yeast species Saccharomyces cerevisiae and its growth medium dried in such a manner that it maintained its fermentative capacity. The addition of YC significantly increased the concentration of acetate (P &lt; 0·05) while propionate concentration tended to be higher for bulls given YC (P &gt; 0·05). The acetate: propionate ratio remained unchanged. Concentration of total volatile fatty acid (VFA) was significantly higher in YC bulls compared with control bulls (P &lt; 0·05). The in vitro studies using the Menke gas test confirmed these findings. Mean in vitro gas production in bulls receiving YC was lower than that in the controls (P &lt; 0·05) and methane production was significantly reduced by the addition of YC after 12h (P &lt; 0·01). Ruminal ammonia concentrations were not affected by treatment but ruminal pH was significantly depressed by the addition of YC (P &lt; 0·05). Apparent digestibility of dry matter, organic matter, crude protein and neutral-detergent fibre were unaffected by treatment but tended to be higher with the control diet. Nitrogen retention was not affected by the addition of YC and mean values for allantoin excretion and plasma urea were similar. In a 28-week feeding trial, dry-matter intake was significantly greater for bulls given YC (5·55 kg/day) than for control bulls (5·32 kg/day, P &lt; 0·05) but average daily gain, 1·55 and 1·58 kg/day for control and YC respectively, and food conversion efficiency were not improved significantly by YC (P &gt; 0·05).
Biochemical and physiological parameters of recovery in acute severe head injury: Responses to multisensory stimulation (1993)

We investigated the efficacy of applying a programme of multisensory stimulation to patients with severe diffuse traumatic brain injury, during their admission to a tertiary neurosurgical intensive care unit. We attempted to determine the nature and extent of any physiological or biochemical changes occurring as a result of the multisensory stimulation in the initial period of their comatose state. The findings were inconclusive with no significant treatment effect demonstrated.
<title>Microchannel coolers for high-power laser diodes in copper technology</title> (1994)

This paper describes as an alternative to the silicon technology the production, assembly, and performance of microchannel coolers in copper technology. A realization technique for these copper coolers is described for one specific cooler geometry with a stacked design of 5 microstructured copper plates. These 5 layers are bonded together by diffusion welding at a temperature near 800 degree(s)C. This bonding process allows the fabrication of 3 X 8 coolers in one step in which the 5 structured copper plates of a size of 100 X 150 mm2 are bonded together. The structuring procedure is currently done by etching and laser machining. Microchannels with a width of 60 - 100 micrometers and a depth of 300 micrometers are used in the experiments as microstructures for better comparability to already existing theoretical and experimental results. A thermal resistance of 0.44 K/W of these copper coolers is demonstrated for a device with a 10 mm laser diode bar mounted on the front edge of the cooler. Experimental results for this configuration reach an average laser diode power of 71.5 Watts from one single bar with a footprint of 0.6 X 10 mm2. The experimental results are compared to 3-D heat flow calculations.© (1994) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
신경인지재활치료가 편측무시환자의 시지각 기능과 상지기능에 미치는 효과 (2014)

목적: 본 연구의 목적은 편측무시를 보이는 좌측 편마비환자에게 신경인지재활치료를 접근하여 편측무시가 감소됨으로 시지각 기능의 향상에 따른 상지기능에 미치는 효과를 확인하고자 하여 향후 신경학적 손상 환자들의 치료적 질을 향상시키는데 기여하고자 한다. 방법: 본 연구의 대상자는 인천에 소재하는 B재활병원에 입원하고 있는 좌측 편마비 환자 중 편측무시를 진단 받은 자로 자연 회복 가능성을 최소화하기 위하여 발병 기간이 6개월 이상 경과하고 실험의 내용을 이해하고 적극적으로 참여 의사를 밝힌 자를 선정하였다. 본 연구의 참여자는 40세 남자로 2014년 1월 4일 집 앞에서 쓰러진 후 발생한 의식 변화로 대학병원으로 후송되어 자기공명영상촬영(Magnetic Resonance Image; MRI)에 의해 뇌내출혈과 경막하출혈으로 진단받은 환자이다. 대상자는 타병원에서 치료를 받다가 전문재활치료를 받기위하여 본원에 2014년 6월 5일 입원하여 2014년 6월 9일부터 2014년 8월 1일까지 8주 동안 주 5회신경인지재활치료를 실시하였다. 결과: 본 연구의 결과를 보면 상지기능은 뇌졸중상지기능검사에서 마비측 22점 향상, 퍼듀 페그보드 검사에서 마비측6개 향상, 그루브드 페그보드 검사와 젭슨-테일러 손 기능검사 마비측 수행불가능에서 수행 가능해졌다. 장악력 10㎏, 편측집기 6.5㎏, 손바닥 집기 5.5㎏, 손끝집기검사 3.5㎏으로 각각 증진 되었다. MVPT-3는 원점수가 17개 향상되었다. 결론: 신경인지재활치료가 편측무시의 감소에 효과가 있었고, 편측무시가 감소됨으로 시지각 기능이 향상되어 상지기능이 향상되는 효과를 나타내었다. 본 연구는 자연회복이 되지 않는 편측무시 환자들의 치료에 활용될 수 있을 것이고 향후 신경학적 손상 환자들의 치료적 질을 향상시키는데 기여할 수 있을 것으로 생각된다.
Overexpression of cyclin D1 in rat fibroblasts causes abnormalities in growth control, cell cycle progression and gene expression. (1993)

Cyclin D1, a putative G1 cyclin, has been implicated in cell cycle control. The human cyclin D1 gene is located on chromosome 11q13 where DNA rearrangement and amplification have been detected in several types of human cancer. Previous studies demonstrated that the cyclin D1 gene is not only rearranged or amplified but also overexpressed in some of these human tumors and tumor-derived cell lines. To further address the roles of cyclin D1 in cell cycle control and tumorigenesis, we have stably overexpressed the human cyclin D1 cDNA in Rat6 embryo fibroblasts by using retrovirus mediated transduction. The cyclin D1 protein was overproduced about 10-fold and was localized predominately in the nucleus. Cyclin D1 overexpressing cells displayed a decrease in the duration of the G1 phase, decreased cell size, and induced tumors when injected into athymic (nude) mice. In addition, overexpression of cyclin D1 in Rat6 cells perturbed the expression of several cellular growth-related genes including c-myc, c-jun, and cyclin A, but not cyclin D3. Taken together, these results indicate that deregulated expression of the cyclin D1 gene can cause disturbances in cell cycle control and gene expression and also enhance tumorigenesis.
Buckling in serpentine microstructures and applications in elastomer-supported ultra-stretchable electronics with high areal coverage (2013)

Lithographically defined electrical interconnects with thin, filamentary serpentine layouts have been widely explored for use in stretchable electronics supported by elastomeric substrates. We present a systematic and thorough study of buckling physics in such stretchable serpentine microstructures, and a strategic design of serpentine layout for ultra-stretchable electrode, via analytical models, finite element method (FEM) computations, and quantitative experiments. Both the onset of buckling and the postbuckling behaviors are examined, to determine scaling laws for the critical buckling strain and the limits of elastic behavior. Two buckling modes, namely the symmetric and anti-symmetric modes, are identified and analyzed, with experimental images and numerical results that show remarkable levels of agreement for the associated postbuckling processes. Based on these studies and an optimization in design layout, we demonstrate routes for application of serpentine interconnects in an ultra-stretchable electrode that offer, simultaneously, an areal coverage as high as 81%, and a biaxial stretchability as large as ~170%.
Plasma prekallikrein levels are positively associated with circulating lipid levels and the metabolic syndrome in children (2010)

Plasma prekallikrein (PK) has been shown to be associated with cardiovascular disease (CVD) and its risk factors, but these associations have not been investigated in children. The present study examined PK activity in relation to well-established cardiovascular risk factors in a cohort of children aged 9-11 years (N=97). We found a significant and positive association between PK and fasting levels of total cholesterol (p<0.01), non-high-density lipoprotein cholesterol (p<0.01), and triglycerides (p<0.001). In addition, there was a significant association between PK activity and the metabolic syndrome, a clustering of risk factors considered to have an impact on atherosclerosis and CVD mortality. Finally, we found that children with a family history of CVD had significantly elevated PK activity. These novel findings warrant further investigations into the relationship between circulating PK levels and CVD risk factors because PK may be involved in the progression of the disease state.
Search for Correlates of Protective Immunity Conferred by Anthrax Vaccine (2001)

Vaccination by anthrax protective antigen (PA)-based vaccines requires multiple immunization, underlying the need to develop more efficacious vaccines or alternative vaccination regimens. In spite of the vast use of PA-based vaccines, the definition of a marker for protective immunity is still lacking. Here we describe studies designed to help define such markers. To this end we have immunized guinea pigs by different methods and monitored the immune response and the corresponding extent of protection against a lethal challenge with anthrax spores. Active immunization was performed by a single injection using one of two methods: (i) vaccination with decreasing amounts of PA and (ii) vaccination with constant amounts of PA that had been thermally inactivated for increasing periods. In both studies a direct correlation between survival and neutralizing-antibody titer was found (r(2) = 0.92 and 0.95, respectively). Most significantly, in the two protocols a similar neutralizing-antibody titer range provided 50% protection. Furthermore, in a complementary study involving passive transfer of PA hyperimmune sera to naive animals, a similar correlation between neutralizing-antibody titers and protection was found. In all three immunization studies, neutralization titers of at least 300 were sufficient to confer protection against a dose of 40 50% lethal doses (LD(50)) of virulent anthrax spores of the Vollum strain. Such consistency in the correlation of protective immunity with anti-PA antibody titers was not observed for antibody titers determined by an enzyme-linked immunosorbent assay. Taken together, these results clearly demonstrate that neutralizing antibodies to PA constitute a major component of the protective immunity against anthrax and suggest that this parameter could be used as a surrogate marker for protection.
ChemInform Abstract: SYNTHESIS AND ANTI‐INFLAMMATORY ACTIVITY OF SOME SUBSTITUTED TETRAZOLES (1980)

Abstract Wie im Formelschema angegeben, werden die Tetrazole (IV) synthetisiert.
Synthesis and X-ray crystal structures of two new alkaline-earth metal borates: SrBO2(OH) and Ba3B6O9(OH)6 (2002)

Using hydrothermal techniques, two new compounds—strontium borate SrBO2(OH) and barium polyborate Ba3B6O9(OH)6—have been prepared. The structures of the title compounds were solved by single-crystal X-ray diffraction analysis. In the structure of SrBO2(OH), the Sr2+ ion is surrounded by eight oxygen atoms and the borate ion is identified as an individual [BO2(OH)]2− group. The structure of Ba3B6O9(OH)6 can be regarded as constructed from anionic borate chains, the negative charges of which are compensated for by the Ba2+ cations residing between the adjacent chains. The unusual feature of this compound can also be described in terms of infinite chains of B3O3 six-membered rings sharing B atoms, which are all tetrahedrally coordinated by oxygen atoms. The materials have also been characterized by infrared and energy dispersive spectroscopies, thermal analysis, and second harmonic generation powder measurement.
Analytic evaluation of shared-memory systems with ILP processors (1998)

This paper develops and validates an analytical model for evaluating various types of architectural alternatives for shared-memory systems with processors that aggressively exploit instruction-level parallelism. Compared to simulation, the analytical model is many orders of magnitude faster to solve, yielding highly accurate system performance estimates in seconds.The model input parameters characterize the ability of an application to exploit instruction-level parallelism as well as the interaction between the application and the memory system architecture. A trace-driven simulation methodology is developed that allows these parameters to be generated over 100 times faster than with a detailed execution-driven simulator.Finally, this paper shows that the analytical model can be used to gain insights into application performance and to evaluate architectural design trade-offs.
DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae. (1986)

We showed by immunofluorescence that the procaryotic DNA-binding protein LexA and a chimeric protein that contains the DNA-binding portion of LexA (amino acids 1 to 87) and a large portion (amino acids 74 to 881) of the Saccharomyces cerevisiae positive regulatory GAL4 protein (GAL4 gene product) are not preferentially localized in the nucleus in S. cerevisiae.
Nonvolatilealpha‐branched chain fatty esters. II (1968)

Abstract A group of nonvolatile alpha ‐branched esters was prepared by the di‐tertiary butyl peroxide‐promoted addition of normal esters to terminal olefins containing methyl branches or aryl groups. Methyl stearate was added to 3,7‐dimethyl‐1‐octene, 3,5,5‐trimethyl‐1‐hexene, and 4‐phenyl‐1‐butene. The reaction with 2,4,4‐trimethyl‐1‐pentene, a terminal alkene with a methyl branch at the internal ethylenic carbon, was not successful. Benzyl 2‐(4‐phenylbutyl) octadecanoate was prepared by transesterification of the corresponding methyl ester. Benzyl 2,2‐dimethylpropanoate, which has no α‐hydrogen in the acyl portion of the molecule, was added to 1‐hexadecene to form α‐hexadecylbenzyl 2,2‐dimethylpropanoate. Lubricant evaluation data were obtained on the above compounds.
HeNe-Laser Light Scattering by Human Dental Enamel (1995)

Knowledge of the optical properties of tooth enamel and an understanding of the origin of these properties are necessary for the development of new optical methods for caries diagnosis and the measurement of tooth color. We measured the scattering intensity functions for HeNe-laser light of 80- to 100- micrometers-thick human dental enamel slabs. The asymmetry factors were calculated to be g = 0.68 at 633 nm. By measuring the collimated beam attenuation, we determined the scattering coefficient to be s = 6.6mm(-1). From Fraunhofer diffraction patterns, obtained from transmission of the laser beam, we calculated the periodicity of the prismatic structure as 5.4 micrometers. We present a model containing scattering by crystals and by prisms. It shows that the prisms are the most important scatterers but that the crystals are responsible for the back-scattering.
<title>New problems of femtosecond time-domain CARS (coherent antistokes Raman spectroscopy) of large molecules</title> (1991)

New possilities appeared in time-domain CARS of molecules due to the application of femtosecond lasers are analyzed. The most important one is that femtosecond pulses allow to control not only amplitude but also the phase of molecular vibrations.
Insulin Mimetic Peroxo Complexes of Vanadium Containing Uracil or Cytosine as Ligand (2000)

Mixed ligand oxo peroxo complexes of vanadium (V), M[VO(O(2))L(2)].nH(2)O where M = K or NH(4), HL = uracil or cytosine and n = 1 or 2, have been isolated from aqueous methanolic medium. The complexes were characterised by elemental analysis, conductance, TGA, UV-Visible, IR and NMR spectral studies. Both the peroxide and the other ligands acts as chelates coordinating through their oxygen at C (2) and nitrogen at N (3) and the presence of monomeric oxoperoxovanadium (V) species, have been established by IR, (1)H and (51)V NMR studies. The complexes appeared to possess pentagonal bipyramidal geometry. The terminal oxo ligand and the oxygen of the uracil or cytosine at C (2) occupy the axial position while the peroxide and the other donor ligands are in the equatorial position. The UV spectral studies confirm the presence of Vanadium in its +5 oxidation state. The administration of the potassium salts of the complexes reduces the blood glucose level in Swiss Albino mice compared to that of KVO(3). The complexes also readily oxidise cysteine to Cystine in aqueous solution. The possible mechanism for the insulin-mimic activity of the complexes is discussed.
Deregulation of histone lysine methyltransferases contributes to oncogenic transformation of human bronchoepithelial cells (2008)

Our results indicate the potential of these HKMTs in addition to the other targets for epigenetics such as DNMTs and HDACs to be interesting therapeutic targets.
The Effect of Cooling Regimens on the Transplantation Potential of Marrow (1967)

These experiments were designed to identify those portions of the cooling curve, variations in which most affect the repopulating potential of thawed marrow. Murine bone marrow was suspended in Hanks' solution containing 12% glycerol and 4% calf serum. One ml samples of this suspension were frozen at controlled cooling rates and after thawing the transplantation potential of each sample was bioassayed. The bioassay was based on the colony producing potential of hematopoietic tissue injected into lethally irradiated mice. The cooling curve was divided into five separate phases each of which was studied independently at several levels. At the levels studied, the rate of cooling to freezing, the duration of the pre‐freezing plateau and the temperature at which freezing was initiated did not affect the repopulating potential of marrow. However, the repopulating potential of marrow decreased in a linear fashion as: (1) the post‐freezing plateau was lengthened or (2) as the rate of cooling from freezing to —100 C was increased.
Using Drug Claims Data to Assess the Relationship of Medication Adherence With Hospitalization and Costs (2001)

The availability of drug claims data and the ability to use them in predictive analyses make them a potentially useful data source in studies of medication adherence among persons with severe mental illness.