gebhart/patrepeval huggingface dataset:
Design and Computed Theoretical Performance of Three Classes of Equal-Ripple Nonuniform Line Couplers (1969)

The purpose of this paper is to provide an accurate theoretical picture of three classes of tapered-line couplers. The coupler taper is approximated with a cascade of short equal-length coupled line segments whose coupling values match those of the taper at one end of each segment. Exact analysis of the even-mode transmission-line analogy is then performed by evaluating the ABCD matrix of the cascade as a function of frequency with the aid of a digital computer. The computed response converges to that of the smooth tapered coupler as the number of line segments increases, total length being held constant. An exact asymptotic high-frequency model for asymmetric couplers is presented which provides improved prediction of coupler performance, as verified by computer analysis. The associated design procedures are given explicitly. The insertion phase dispersion of the 90/spl deg/ and /spl Sigma/ - /spl Delta/ magic tee devices is treated. Comparisons with the insertion phase dispersion of various stepped coupling 90/spl deg/ designs are provided.
Effects of Antiflammins on Endotoxin-Induced Uveitis in Rats (1991)

Antiflammins are phospholipase A2-inhibitory, anti-inflammatory, synthetic oligopeptides derived from the region of the highest amino-acid sequence similarity between uteroglobin and lipocortin I. Endotoxin-induced uveitis is a model for anterior uveitis of the eye, which has been suggested to be induced through phospholipase A2 activation. In a preliminary report we demonstrated that topical administration of antiflammins could inhibit endotoxin-induced uveitis in rats. In this study, the anti-inflammatory effects of antiflammins were compared with those of corticosteroids on endotoxin-induced uveitis as measured by phospholipase A2 enzyme activity, inflammatory cell counts in the aqueous humor, and histopathologic features. Antiflammins are as effective as corticosteroids in their ability to suppress endotoxin-induced uveitis.
Reproducible switching effect in thin oxide films for memory applications (2000)

Thin oxide films with perovskite or related structures and with transition metal doping show a reproducible switching in the leakage current with a memory effect. Positive or negative voltage pulses can switch the resistance of the oxide films between a low- and a high-impedance state in times shorter than 100 ns. The ratio between these two states is typically about 20 but can exceed six orders of magnitude. Once a low-impedance state has been achieved it persists without a power connection for months, demonstrating the feasibility of nonvolatile memory elements. Even multiple levels can be addressed to store two bits in such a simple capacitor-like structure.
Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I (2006)

The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found.
Tyrosine-O-sulfated proteins of PC12 pheochromocytoma cells and their sulfation by a tyrosylprotein sulfotransferase. (1983)

The O-sulfation of specific proteins on tyrosine residues was studied using the rat pheochromocytoma cell line PC12 as a model system. In intact PC12 cells labeled with inorganic [35S]sulfate, the major protein substrates for sulfation on tyrosine were four acidic polypeptides with apparent molecular weights of 113,000, 105,000, 86,000, and 84,000 designated as p113, p105, p86, and p84. After labeling of intact PC12 cells with inorganic [32P]phosphate, these four proteins were also found to be phosphorylated at serine residues. Peptide mapping after limited proteolysis indicated sequence homologies between p113 and p105, and between p86 and p84. In lysed PC12 cells, p113, p105, p86, and p84 were phosphorylated at serine residues by an endogenous protein kinase using [32P] ATP. Moreover, in the cell-free preparation, an enzymatic activity was detected that was able to catalyze the sulfation of the four proteins on tyrosine residues. This sulfation reaction, which used adenosine 3'-phosphate 5'-phospho[35S]sulfate as the sulfate donor, occurred in a particulate fraction of PC12 cells and was inhibited by 5 mM EDTA. These results demonstrate the presence in PC12 cells of a novel enzyme, designated here as a tyrosylprotein sulfotransferase, and imply a role for this enzyme in the post-translational processing of specific PC12 cell proteins.
Nucleic acid aptamers in cancer medicine (2002)

Many signalling proteins involved in diverse functions such as cell growth and differentiation can act as oncogenes and cause cellular transformation. These molecules represent attractive targets for cancer diagnosis or therapy and are therefore subject to intensive investigation. Aptamers are small nucleic acid molecules, isolated from combinatorial libraries by a procedure termed SELEX, that bind to a target molecule by providing a limited number of specific contact points embedded in a larger, defined three-dimensional structure. In some cases aptamers have the potential to inhibit the biological function of the molecule resulting in useful reagents for target validation in a variety of disease models.
Experimental Analysis of Pattern and Polarization Reconfigurable Circular Patch Antennas for MIMO Systems (2010)

In this paper, we investigate the performance achievable with pattern and polarization multielement reconfigurable antennas in narrowband and broadband multiple-input-multiple-output (MIMO) systems by means of two-port reconfigurable circular patch antennas (RCPAs). We use field measurements collected in an indoor environment to determine the channel capacity achievable with the RCPAs when used at one or at both ends of the communication link. To conduct this analysis, we use two types of RCPAs: 1) an RCPA with two different antenna configurations, exploiting only pattern diversity and 2) an RCPA with three different antenna configurations, exploiting both pattern and polarization diversity. The benefits offered by each state (i.e., excited radiation pattern and polarization) of the RCPAs are investigated in both line-of-sight (LOS) and nonline-of-sight (NLOS) scenarios. The analysis shows the effectiveness of each array configuration in increasing the diversity level of the system and the amount of signal power received in LOS and NLOS communication links. The radiation efficiency and the level of pattern and polarization diversity of each configuration are investigated to explain the performance offered by this class of antennas in MIMO systems. Finally, we show the difference in performance achievable with these antennas when employed in narrowband or broadband communication systems.
Functional Roles of Sphingosine, Sphingosine 1-Phosphate, and Methylsphingosines: In Regard to Membrane Sphingolipid Signaling Pathways (1997)

The signaling roles of ceramide and sphingosine produced through the degrading processes of membrane sphingolipids are currently receiving hot attention in the biochemical and biomedical research fields. For these 9 years at the Biomembrane Institute in Seattle, we have studied functional roles of various sphingolipids such as ceramide, sphingosine, methylsphingosines, and sphingosine 1-phosphate in a variety of biomedical systems. In this article, first, the recent conceptual developments on sphingolipid signaling pathways is outlined. Next, our recent findings on the functional roles of sphingolipids are described focusing on (i) functional roles of sphingosine 1-phosphate in cell motility regulation and platelet activation (ii) involvement of sphingosine in cell signaling (iii) effects of methylsphingosines in cancer cell apoptosis induction and in the regulation of inflammatory processes. Based upon these findings from our studies and others, the perspective of future sphingosine research (sphingology or sphingophysiology) is briefly discussed.
Neuroprotective efficacy of aminopropyl carbazoles in a mouse model of amyotrophic lateral sclerosis (2012)

We previously reported the discovery of P7C3, an aminopropyl carbazole having proneurogenic and neuroprotective properties in newborn neural precursor cells of the hippocampal dentate gyrus. We have further found that chemicals having efficacy in this in vivo screening assay also protect dopaminergic neurons of the substantia nigra following exposure to the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, a mouse model of Parkinson disease. Here, we provide evidence that an active analog of P7C3, known as P7C3A20, protects ventral horn spinal cord motor neurons from cell death in the G93A-SOD1 mutant mouse model of amyotrophic lateral sclerosis (ALS). P7C3A20 is efficacious in this model when administered at disease onset, and protection from cell death correlates with preservation of motor function in assays of walking gait and in the accelerating rotarod test. The prototypical member of this series, P7C3, delays disease progression in G93A-SOD1 mice when administration is initiated substantially earlier than the expected time of symptom onset. Dimebon, an antihistaminergic drug with significantly weaker proneurogenic and neuroprotective efficacy than P7C3, confers no protection in this ALS model. We propose that the chemical scaffold represented by P7C3 and P7C3A20 may provide a basis for the discovery and optimization of pharmacologic agents for the treatment of ALS.
The PYRIN-CARD Protein ASC Is an Activating Adaptor for Caspase-1 (2002)

The PYRIN and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the apoptotic and inflammatory signaling pathways. Here we show that the PYRIN-CARD protein ASC functions as a caspase-1-activating adaptor. ASC interacted specifically with procaspase-1 via CARD-CARD interactions and induced its oligomerization. Consistent with these results ectopic expression of full-length ASC, but not its isolated CARD or PYRIN domain, with procaspase-1 induced activation of procaspase-1 and processing of pro-interleukin-1beta in transfected cells. Substitution of the PYRIN domain of ASC with an inducible FKBP12 oligomerization domain produced a molecule that can induce caspase-1 activation in response to stimulation with the oligomerization drug AP20187, suggesting that the PYRIN domain functions as an oligomerization domain, whereas the CARD domain functions as the effector domain in the caspase-1 activation pathway. Furthermore stable expression of an isolated CARD of ASC in THP-1 cells diminished interleukin-1beta generation in response to pro-inflammatory cytokines. These results indicate that ASC is involved in the caspase-1 signaling pathway by mediating the assembly of a caspase-1-inflammasome signaling complex in response to pro-inflammatory cytokine stimulation.
Gene Therapy for Infectious Diseases (1998)

Gene therapy is being investigated as an alternative treatment for a wide range of infectious diseases that are not amenable to standard clinical management. Approaches to gene therapy for infectious diseases can be divided into three broad categories: (i) gene therapies based on nucleic acid moieties, including antisense DNA or RNA, RNA decoys, and catalytic RNA moieties (ribozymes); (ii) protein approaches such as transdominant negative proteins and single-chain antibodies; and (iii) immunotherapeutic approaches involving genetic vaccines or pathogen-specific lymphocytes. It is further possible that combinations of the aforementioned approaches will be used simultaneously to inhibit multiple stages of the life cycle of the infectious agent.
The FokI Restriction-Modification System (1989)

A DNA fragment that carried the genes coding for FokI endonuclease and methylase was cloned from the chromosomal DNA of Flavobacterium okeanokoites, and the coding regions were assigned to the nucleotide sequence by deletion analysis. The methylase gene was 1,941 base pairs (bp) long, corresponding to a protein of 647 amino acid residues (Mr = 75,622), and the endonuclease gene was 1,749 bp long, corresponding to a protein of 583 amino acid residues (Mr = 66,216). The assignment of the methylase gene was further confirmed by analysis of the N-terminal amino acid sequence. The endonuclease gene was downstream from the methylase gene in the same orientation, separated by 69 bp. The promoter site, which could be recognized by Escherichia coli RNA polymerase, was upstream from the methylase gene, and the sequences adhering to the ribosome-binding sequence were identified in front of the respective genes. Analysis of the gene products expressed in E. coli cells by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the molecular weights of both enzymes coincided well with the values estimated from the nucleotide sequences, and that the monomeric forms were catalytically active. No significant similarity was found between the sequences of the two enzymes. Sequence comparison with other related enzymes indicated that FokI methylase contained two copies of a segment of tetra-amino acids which is characteristic of adenine-specific methylase.
VGGFace2: A dataset for recognising faces across pose and age (2017)

In this paper, we introduce a new large-scale face dataset named VGGFace2. The dataset contains 3.31 million images of 9131 subjects, with an average of 362.6 images for each subject. Images are downloaded from Google Image Search and have large variations in pose, age, illumination, ethnicity and profession (e.g. actors, athletes, politicians). The dataset was collected with three goals in mind: (i) to have both a large number of identities and also a large number of images for each identity; (ii) to cover a large range of pose, age and ethnicity; and (iii) to minimize the label noise. We describe how the dataset was collected, in particular the automated and manual filtering stages to ensure a high accuracy for the images of each identity. To assess face recognition performance using the new dataset, we train ResNet-50 (with and without Squeeze-and-Excitation blocks) Convolutional Neural Networks on VGGFace2, on MS- Celeb-1M, and on their union, and show that training on VGGFace2 leads to improved recognition performance over pose and age. Finally, using the models trained on these datasets, we demonstrate state-of-the-art performance on all the IARPA Janus face recognition benchmarks, e.g. IJB-A, IJB-B and IJB-C, exceeding the previous state-of-the-art by a large margin. Datasets and models are publicly available.
Substrate-bias effect and source-drain breakdown characteristics in body-tied short-channel SOI MOSFET's (1999)

The substrate-bias effect and source-drain breakdown characteristics in body-tied short-channel silicon-on-insulator metal oxide semiconductor field effect transistors (SOI MOSFET's) were investigated. Here, "substrate bias" is the body bias in the SOI MOSFET itself. It was found that the transistor body becomes fully depleted and the transistor is released from the substrate-bias effect, when the body is reverse-biased. Moreover, it was found that the source-drain breakdown voltage for reverse-bias is as high as that for zero-bias. This phenomenon was analyzed using a three-dimensional (3-D) device simulation considering the body-tied SOI MOSFET structure in which the body potential is fixed from the side of the transistor. This analysis revealed that holes which are generated in the transistor are effectively pulled out to the body electrode, and the body potential for reverse-bias remains lower than that for zero-bias, and therefore, the source-drain breakdown characteristics does not deteriorate for reverse-bias. Further, the influence of this effect upon circuit operation was investigated. The body-tied configuration of SOI devices is very effective in exploiting merits of SOI and in suppressing the floating body-effect, and is revealed to be one of the most promising candidates for random logic circuits such as gate arrays and application specific integrated circuits.
Altered serotonin physiology in human breast cancers favors paradoxical growth and cell survival (2009)

Our data constitutes the first report of direct involvement of 5-HT in human breast cancer. Increased 5-HT biosynthetic capacity accompanied by multiple changes in 5-HT receptor expression and signaling favor malignant progression of human breast cancer cells (for example, stimulated proliferation, inappropriate cell survival). This occurs through uncoupling of serotonin from the homeostatic regulatory mechanisms of the normal mammary epithelium. The findings open a new avenue for identification of diagnostic and prognostic markers, and valuable new therapeutic targets for managing breast cancer.
FILOCHAT (1994)

Article Free Access Share on FILOCHAT: handwritten notes provide access to recorded conversations Authors: Steve Whittaker Lotus Development Corporation, 1 Rogers St., Cambridge, MA and Office Appliances Dept., Hewlett-Packard Laboratories, Filton Road, Bristol, BS12 6QZ, UK Lotus Development Corporation, 1 Rogers St., Cambridge, MA and Office Appliances Dept., Hewlett-Packard Laboratories, Filton Road, Bristol, BS12 6QZ, UKView Profile , Patrick Hyland Office Appliances Dept., Hewlett-Packard Laboratories, Filton Road, Bristol, BS12 6QZ, UK Office Appliances Dept., Hewlett-Packard Laboratories, Filton Road, Bristol, BS12 6QZ, UKView Profile , Myrtle Wiley Office Appliances Dept., Hewlett-Packard Laboratories, Filton Road, Bristol, BS12 6QZ, UK Office Appliances Dept., Hewlett-Packard Laboratories, Filton Road, Bristol, BS12 6QZ, UKView Profile Authors Info & Claims CHI '94: Proceedings of the SIGCHI Conference on Human Factors in Computing SystemsApril 1994 Pages 271–277https://doi.org/10.1145/191666.191763Published:24 April 1994Publication History 77citation1,466DownloadsMetricsTotal Citations77Total Downloads1,466Last 12 Months208Last 6 weeks26 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Effect of purified Clostridium difficile toxins on intestinal smooth muscle. I. Toxin A (1989)

In these studies we determined the effects of purified Clostridium difficile toxin A, an enterotoxin, on the electrophysiological and contractile properties of rabbit intestinal circular smooth muscle and correlated these effects with changes of smooth muscle morphology. Simultaneous measurements of intracellular membrane potential and contractility were determined in excised ileal muscle strips after administration of toxin A in vivo (60 micrograms/ml) into an isolated rabbit ileal loop or directly in vitro (0.1-60 micrograms/ml) to a normal muscle strip. Toxin A injection in vivo resulted in membrane depolarization and increased slow wave and action potential frequency. Toxin A injection in vivo also caused increased amplitude of spontaneous and carbachol-induced phasic contractions. The electrophysiological effects of in vivo administration of toxin A were correlated with an inflammatory infiltrate of the lamina propria, but no light or electron microscopic evidence of injury to smooth muscle cells was seen. In contrast to the in vivo studies, direct in vitro exposure of normal ileal muscle strips to toxin A had no effect on either spontaneous or carbachol-induced electromechanical activity. Our results indicate that in vivo administration of C. difficile toxin A into a rabbit ileal loop, but not direct in vitro exposure, causes significant alterations of smooth muscle excitation-contraction coupling that may be mediated by products of local inflammatory cells.
Determination of the amounts of the protein synthesis initiation and elongation factors in wheat germ. (1990)

Previous work by Browning et al. (Browning, K. S., Lax, S. R., Humphreys, J., Ravel, J. M., Jobling, S. A., and Gehrke, L. (1988) J. Biol. Chem. 263, 9630-9634) indicated that wheat germ extracts do not contain sufficient amounts of some of the protein synthesis initiation factors to obtain optimal translation of all mRNAs. In this investigation, a quantitative enzyme-linked immunosorbent assay was used to determine the amounts of eukaryotic initiation factors (eIF) 2, 3, 4A, 4F, and (iso)4F as well as the amounts of 40 S ribosomal subunits and elongation factors (EF) 1 alpha and 2 present in wheat germ extracts. EF-1 alpha is present in the highest amount (approximately 5% of the total protein), and eIF-4F is present in the lowest amount (approximately 0.03% of the total protein). The micromolar amounts of the factors and ribosomes are as follows: EF-1 alpha, 34; EF-2, 5.2; eIF-2, 1.5; eIF-3, 0.7; eIF-4A, 3.0, eIF-4F, 0.09; eIF-(iso)4F, 0.8; and 40 S ribosomal subunits, 3.2. The molar ratios of the factors to 40 S ribosomal subunits are approximately 11:1 for EF-1 alpha, 1.6:1 for EF-2, 0.45:1 for eIF-2, 0.2:1 for eIF-3, 0.9:1 for eIF-4A, 0.03:1 for eIF-4F, and 0.25:1 for eIF-(iso)4F. These findings strongly suggest that the concentrations of the initiation factors, particularly those factors required for the binding of mRNA to ribosomes, may play a major role in regulating the translation of mRNAs within the cell.
The K+ Channel Opener 1-EBIO Potentiates Residual Function of Mutant CFTR in Rectal Biopsies from Cystic Fibrosis Patients (2011)

We conclude that 1-EBIO potentiates Cl⁻secretion in native CF tissues expressing CFTR mutants with residual Cl⁻ channel function by activation of basolateral KCNN4 K⁺ channels that increase the driving force for luminal Cl⁻ exit. This mechanism may augment effects of CFTR correctors and potentiators that increase the number and/or activity of mutant CFTR channels at the cell surface and suggests KCNN4 as a therapeutic target for CF.
先驱与先烈——月入数万的“倍增事业” (2010)

金融海啸，不少人一夜之间失去很多资产财富，于是奥斯卡大奖片《贫民富翁》备受追捧。与此同时，一位“中国版贫民富翁”林A，也成为最近网络关注的人物。我们几经周折了解到，林A是一位企业家，其创业和成功经历颇具传奇色彩。