gebhart/patrepeval huggingface dataset:
Analysis of short-term NBTI effect on the Single-Event Upset sensitivity of a 65nm SRAM using two-photon absorption (2013)

We used two-photon absorption laser testing to characterize the effect of short-term NBTI aging on the SEU sensitivity of 65 nm CMOS SRAM cell. Results indicate a fast increase of SEU sensitivity due to aging.
Thermostabilization of firefly luciferase by a single amino acid substitution at position 217 (1993)

Random mutagenesis of the luciferase cDNA from "Genji" firefly, Luciola cruciata, was induced by hydroxylamine in an attempt to isolate thermostable mutants. Three mutants were isolated, and the cDNAs encoding these proteins were sequenced. All mutant cDNAs carried the same C to T transition mutation that conferred an amino acid substitution of Thr by Ile at position 217. The wild-type luciferase and the thermostable variant (Thr217Ile) were purified to homogeneity, and their enzymatic properties were determined. Thr217Ile was superior to wild-type in thermal and pH stability. Furthermore, the specific activity of the Thr217Ile mutant was increased to 130% of that of the wild-type. In order to examine the effect of amino acid residue substitution at position 217 on the thermostability of luciferase, we replaced the Thr residue at position 217 with all of the rest of the possible amino acid residues by site-directed mutagenesis. The thermostability of these substitution mutants seemed to correlate with the hydrophobicity of the substituted amino acid residue.
901. Polyfluoroalkyl compounds of silicon. Part IV. Polyfluoroalkyl-silanes, -silicones, and -polysiloxanes derived from perfluoroalkylethylenes or vinylsilanes (1957)

A. M. Geyer, R. N. Haszeldine, K. Leedham and R. J. Marklow, J. Chem. Soc., 1957, 4472 DOI: 10.1039/JR9570004472
Automating and streamlining inference of function of plant ESTs within a data analysis system (1994)

Automated sequencing of partial cDNA clones as expressed sequence tags (ESTs) and similarity searches of these ESTs against public DNA and protein sequence databanks is becoming a well-accepted method for identifying genes of any given species. The use of local similarity search algorithms against the public databanks has been preferred as a screening mechanism for the short EST sequences, because they determine scores for only those regions which are conserved between sequences. Furthermore, local similarities of translated EST sequences to the public protein databank sequences can often be detected when similarities to the public DNA databanks appear coincidental. Both human brain and Caenorhabditis elegans cDNA EST projects have used similarity searches for positive identification of known genes and determination of putative function of others. Possible novel genes make up those ESTs which did not show significant similarity to the public databases. The authors show that shorter ESTs are sufficient to meet EST project objectives for plant genomes.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The Rites and Music Tradition (2009)

This chapter traces the major themes of Chinese aesthetics (beauty and goodness, society and nature, feeling and form, art and politics) back to the pre-Confucian tradition of rites and music. It considers the possibility of uniting the statement, “when a ram is large, it is beautiful” (equating beauty with the natural or sensuous), with the statement that “man with ram is beautiful” (equating beauty with goodness). It details how further development and specialization of ancient totemic dance and shamanistic ritual led to the so-called rites and music and laid the foundation for the unity of sense and reason, nature and society, that would come to characterize the Chinese aesthetic tradition. It also discusses the connection between taste and beauty and how rites and music are intimately connected with politics. This chapter suggests that Confucian aesthetics carried on and developed the rites and music tradition of China.
Exploring the specificity of the PI3K family inhibitor LY294002 (2007)

The PI3Ks (phosphatidylinositol 3-kinases) regulate cellular signalling networks that are involved in processes linked to the survival, growth, proliferation, metabolism and specialized differentiated functions of cells. The subversion of this network is common in cancer and has also been linked to disorders of inflammation. The elucidation of the physiological function of PI3K has come from pharmacological studies, which use the enzyme inhibitors Wortmannin and LY294002, and from PI3K genetic knockout models of the effects of loss of PI3K function. Several reports have shown that LY294002 is not exclusively selective for the PI3Ks, and could in fact act on other lipid kinases and additional apparently unrelated proteins. Since this inhibitor still remains a drug of choice in numerous PI3K studies (over 500 in the last year), it is important to establish the precise specificity of this compound. We report here the use of a chemical proteomic strategy in which an analogue of LY294002, PI828, was immobilized onto epoxy-activated Sepharose beads. This affinity material was then used as a bait to fish-out potential protein targets from cellular extracts. Proteins with high affinity for immobilized PI828 were separated by one-dimensional gel electrophoresis and identified by liquid chromatography-tandem MS. The present study reveals that LY294002 not only binds to class I PI3Ks and other PI3K-related kinases, but also to novel targets seemingly unrelated to the PI3K family.
Gate-channel capacitance characteristics in the fully-depleted SOI MOSFET (2001)

A gate-channel capacitance minimum occurs in the capacitance-voltage (C-V) curve of a fully-depleted SOI MOSFET, when the front silicon surface is biased into accumulation while the back surface is maintained in strong inversion. This observation is explained in terms of a model based on the depletion width of the silicon film, taking into account the small accumulation and inversion layer thickness. A simple method is proposed to determine the flat-band potential in the SOI MOSFET.
Network Plus (1988)

Over the past several years, the Electronic Publishing Group at the MIT Media Laboratory has been conducting a family of media experiments which explore a new kind of broadcast: the distribution of data and computer programs rather than pre-packaged material. This broadcast is not directed to a human recipient, but to a local computational agent acting on his behalf. In response to instructions from both the broadcaster and the reader, this agent selects from the incoming data and presents it in a manner suggestive of traditional media. The embodiment of these media experiments is a news retrieval system where the news editor has been replaced by the personal computer. A variety of both local and remote databases which operate passively as well as interac-tively are accessed by &quot;reporters.&quot; These &quot;reporters&quot; are actually software interfaces, which are programmed to gather news. Ideally, they are &quot;broadcatching&quot;; that is to say, watching all broadcast television channels, listening to all radio transmissions, and reading all newspapers, magazines, and journals. 1 A possible consequence of the synthesis of media through active processing is the merger of newspapers and television (figure 1). The result is either a newspaper with illustrations which move 2 or, conversely, print as television output. The latter is the theme of Network Plus.
Identification of albumin-binding proteins in capillary endothelial cells. (1988)

Isolated fat tissue microvessels and lung, whose capillary endothelia express in situ specific binding sites for albumin, were homogenized and subjected to SDS-gel electrophoresis and electroblotting. The nitrocellulose strips were incubated with either albumin-gold (Alb-Au) and directly visualized, or with [125I]albumin (monomeric or polymeric) and autoradiographed. The extracts of both microvascular endothelium and the lung express albumin-binding proteins (ABPs) represented by two pairs of polypeptides with major components of molecular mass 31 and 18 kD. The ABP peptides have pIs 8.05 to 8.75. Rabbit aortic endothelium, used as control, does not express detectable amounts of ABPs. The ABPs subjected to electrophoresis bind specifically and with high affinity (Kd = approximately 60 X 10(-9)M) both monomeric and polymeric albumin: the binding is saturable at approximately 80 nM concentration and 50% inhibition is reached at 5.5 micrograms/ml albumin concentration. Sulfhydryl-reducing agents beta-mercaptoethanol and dithiothreitol do not markedly affect the ABPs electrophoretic mobility and binding properties. As indicated by cell surface iodination of isolated capillary endothelium followed by electroblotting, autoradiography, and incubation with Alb-Au, the bands specifically stained by this ligand are also labeled with radioiodine.
Characterization of human blood dendritic cell subsets (2002)

Dendritic cells (DCs) are key antigen-presenting cells for stimulating immune responses and they are now being investigated in clinical settings. Although defined as lineage-negative (Lin(-)) HLA-DR(+) cells, significant heterogeneity in these preparations is apparent, particularly in regard to the inclusion or exclusion of CD14(+), CD16(+), and CD2(+) cells. This study used flow cytometry and a panel of monoclonal antibodies (mAbs), including reagents from the 7th Leukocyte Differentiation Antigen Workshop, to define the cellular composition of 2 standardized peripheral blood mononuclear cell (PBMCs)-derived Lin(-) HLA-DR(+) preparations. Lin(-) cells were prepared from PBMCs by depletion with CD3, CD14, CD19, CD11b, and either CD16 or CD56 mAbs. Analysis of the CD16-replete preparations divided the Lin(-) HLA-DR(+) population into 5 nonoverlapping subsets (mean +/- 1 SD): CD123 (mean = 18.3% +/- 9.7%), CD1b/c (18.6% +/- 7.6%), CD16 (49.6% +/- 8.5%), BDCA-3 (2.7% +/- 1.4%), and CD34 (5.0% +/- 2.4%). The 5 subsets had distinct phenotypes when compared with each other, monocytes, and monocyte-derived DCs (MoDCs). The CD85 family, C-type lectins, costimulatory molecules, and differentiation/activation molecules were also expressed differentially on the 5 Lin(-) HLA-DR(+) subsets, monocytes, and MoDCs. The poor viability of CD123(+) DCs in vitro was confirmed, but the CD16(+) CD11c(+) DC subset also survived poorly. Finally, the individual subsets used as stimulators in allogeneic mixed leukocyte reactions were ranked by their allostimulatory capacity as CD1b/c > CD16 > BDCA-3 > CD123 > CD34. These data provide an opportunity to standardize the DC populations used for future molecular, functional and possibly even therapeutic studies.
Si Nanowire Bridges in Microtrenches: Integration of Growth into Device Fabrication (2005)

Silicon nanowire bridges are grown in prefabricated microtrenches on (110) silicon-on-insulator wafers (see Figure). Silicon trenches are used as substrates during growth and probing electrodes after growth. This way, nanowire growth and device fabrication can be achieved simultaneously, providing a simple and rational way to realize nanowire-based integrated circuits.
Combined Functional Genome Survey of Therapeutic Targets for Hepatocellular Carcinoma (2010)

The four genes we identified were highly expressed in HCC, and HCC cells are highly dependent on these genes for proliferation. Although many important genes must have been overlooked, the selected genes were biologically relevant. The combination of genome-wide expression and functional screening described here is a rapid and comprehensive approach that could be applied in the identification of therapeutic targets in any type of human malignancy.
Phase retrieval in low-coherence interferometric microscopy (2007)

We present compensating methods that address inherent errors in quantitative phase reporting for low-coherence interferometric techniques. A brief theoretical treatment of the problem and experimental validation using spectral domain phase microscopy demonstrate mitigation of the degrading effects of phase leakage on accurate measurement of optical path length in the vicinity of closely spaced reflectors. This result has direct implications for phase-sensitive interferometry techniques, such as Doppler imaging, as well as amplitude-based quantitative reporting. Corrected phase retrieval is demonstrated for conversion of interferometric phase to optical path length in cell surface deflections of beating cardiomyocytes.
FastHap: fast and accurate single individual haplotype reconstruction using fuzzy conflict graphs (2014)

An implementation of FastHap is available for sharing on request.
Festival Speech Synthesis System (2000)

A general toolkit and full TTS enginefor text to speech supporting a numberof different APIs. Currentlysupports, English - US and UK- andSpanish, though other voices are beingdeveloped
Computationally efficient turbo decoding with the bi-directional Viterbi algorithm (BIVA) (2002)

An algorithm for decoding parallel concatenated codes (turbo codes) that replaces the BCJR algorithm with a new Viterbi type algorithm is presented. Replacement of the BCJR algorithm with the new algorithm results in a gain in computational efficiency with only a slight loss in BER performance.
TGF-β, IL-6, IL-17 and CTGF direct multiple pathologies of chronic cardiac allograft rejection (2010)

Cardiac transplantation is an effective treatment for heart failure refractive to therapy. Although immunosuppressive therapeutics have increased first year survival rates, chronic rejection remains a significant barrier to long-term graft survival. Chronic rejection manifests as patchy interstitial fibrosis, vascular occlusion and progressive loss of graft function. Recent evidence from experimental and patient studies suggests that the development of cardiomyocyte hypertrophy is another hallmark of chronic cardiac allograft rejection. This pathologic hypertrophy is tightly linked to the immune cytokine IL-6, which promotes facets of chronic rejection in concert with TGF-beta and IL-17. These factors potentiate downstream mediators, such as CTGF, which promote the fibrosis associated with the disease. In this article, we summarize contemporary findings that have revealed several elements involved in the induction and progression of chronic rejection of cardiac allografts. Further efforts to elucidate the interplay between these factors may direct the development of targeted therapies for this disease.
Additive-Free Clicking for Polymer Functionalization and Coupling by Tetrazine–Norbornene Chemistry (2011)

Herein we report the use of a tetrazine-norbornene inverse electron demand Diels-Alder conjugation applied to polymer end-functionalization and polymer-polymer coupling. The reaction was found to be applicable to polymer-polymer coupling, as judged by SEC, DOSY NMR, and LCxSEC analyses, giving diblock copolymers by merely mixing the constituent homopolymers together under ambient conditions, using no catalyst, additive, or external stimulus.
Early and sustained effects of cardiac resynchronization therapy on N-terminal pro-B-type natriuretic peptide in patients with moderate to severe heart failure and cardiac dyssynchrony (2007)

CRT exerts an early and sustained reduction in NT-pro-BNP. This appears to reflect improvements in ventricular function. NT-pro-BNP may be a simple method for monitoring the effects of CRT.
Mouse-human chimeric anti-epidermal growth factor receptor antibody C225 inhibits the growth of human renal cell carcinoma xenografts in nude mice. (1998)

The epidermal growth factor (EGF) receptor and its ligand transforming growth factor-alpha (TGF-alpha) are overexpressed in human renal cell carcinoma (RCC). The chimeric anti-EGF receptor monoclonal antibody C225 was used to determine the effects of blocking the EGF receptor on RCC growth both in vitro and in vivo. A panel of RCC cell lines all tested positive at various levels for EGF receptor cell surface expression. C225 inhibited DNA synthesis of cultured A498, Caki-1, SK-RC-4, SK-RC-29, and SW839 cells in a dose-dependent manner, ranging from 20 to 45% inhibition compared with untreated controls. C225 also inhibited exogenous ligand-stimulated tyrosine phosphorylation of EGF receptor on RCC cells. The antitumor effects of C225 on RCC tumor growth were evaluated in ascites, s.c., and orthotopic RCC xenograft models. Mice treated with C225 in a Caki-1 ascites xenograft model showed a significant increase in survival (P = 0.002). All control mice died with ascites tumors by week 9, whereas >70% of C225-treated mice survived beyond 12 weeks. C225 also inhibited the growth of s.c. SK-RC-29 tumors in a dose-dependent manner. Mice treated with C225 (1 mg/dose) displayed a significant decrease in tumor volume compared with mice treated with control antibody (P < 0.05) or vehicle alone (P < 0.01). Lastly, C225 inhibited the growth and metastasis of RCC tumors growing orthotopically in the renal subcapsule of nude mice. Histological examination of RCC tumors from mice treated with C225 showed a substantial decrease in proliferating cell nuclear antigen staining and an increase in tumor cell apoptosis. These data suggest that C225 affects growth of RCC tumors by inhibiting EGF receptor-dependent proliferation and demonstrate the potential for therapeutic application of C225 in the treatment of human renal cancer.
Preparation and mass spectrometric study of egg yolk antibody (IgY) against rabies virus (2001)

Rabies virus was used as the antigen to immunize laying chickens. Anti-rabies virus immunoglobulin Y(IgY) was isolated from yolks of the eggs laid by these chickens using a two-step salt precipitation and one-step gel filtration protocol. The purified IgY was reduced with dithiothreitol, and heavy chains (HC) and light chains (LC) were obtained. In addition, the purified IgY was digested with pepsin and the fragment with specific antigen binding properties (Fab) was produced. Using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOFMS), the average molecular weights of IgY, HC, LC, and Fab were determined as 167 250, 65 105, 18 660, and 45,359 Da, respectively. IgY has two structural differences compared with mammalian IgGs. First, the molecular weight of the heavy chain of IgY is larger than that of its mammalian counterpart, while the molecular weight of the light chain of IgY is smaller. Second, upon pepsin digestion, anti-rabies virus IgY is degraded into Fab, in contrast to mammalian IgG, which has been reported to be degraded into F(ab')(2) under the same conditions.
2‘-C-Methyl Analogues of Selective Adenosine Receptor Agonists:  Synthesis and Binding Studies (1998)

2'-C-Methyl analogues of selective adenosine receptor agonists such as (R)-PIA, CPA, CCPA, NECA, and IB-MECA were synthesized in order to further investigate the subdomain that binds the ribose moiety. Binding affinities of these new compounds at A1 and A2A receptors in bovine brain membranes and at A3 in rat testis membranes were determined and compared. It was found that the 2'-C-methyl modification resulted in a decrease of the affinity, particularly at A2A and A3 receptors. When such modification was combined with N6-substitutions with groups which induce high potency and selectivity at A1 receptors, the high affinity was retained and the selectivity was increased. Thus, 2-chloro-2'-C-methyl-N6-cyclopentyladenosine (2'-Me-CCPA), which displayed a Ki value of 1.8 nM at A1 receptors, was selective for A1 vs A2A and A3 receptors by 2166- and 2777-fold, respectively, resulting in one of the most potent and A1-selective agonists so far known. In functional assay, this compound inhibited forskolin-stimulated adenylyl cyclase activity with an IC50 value of 13.1 nM, acting as a full agonist.
Validation of finite element model of transcranial electrical stimulation using scalp potentials: implications for clinical dose (2013)

Our results provide the first direct model validation for these subject-specific modeling approaches. In addition, the monitoring of scalp voltages may be used to verify electrode placement to increase transcranial electrical stimulation safety and reproducibility.
The importance of intumescent systems for fire protection of plastic materials (2000)

A review of recent developments in the applications and actions of intumescent fire-retardance is given. An attempt has been made to classify the main systems of importance such as melamine, ammonium polyphosphate, melamine phosphate, pentaerythritol phosphate, sodium silicate, vermiculite, expandable graphite and microbeads. They are defined in terms of the Berthelot number which is the product of heat of vaporization or decomposition and volume of gases evolved. In principle, only two kinds of gases are produced from this group, namely water vapour and ammonia (from melamine). The heats of decomposition are readily calculated from heats of formation. An important aspect which is not included in the Berthelot number is the ignition residue in the shape of glassy foam or a cellular enamel. © 2000 Society of Chemical Industry
Properties and Ultrastructure of Phycoerythrin From Porphyridium cruentum (1969)

Phycoerythrin, a photosynthetic accessory pigment, was isolated from Porphyridium cruentum and examined by electron microscopy and disc gel electrophoresis. The absorption monomer, with maxima at 563, 545, and a shoulder at 500 nm, has a molecular weight of about 300,000. With phosphotungstic acid staining it appears as a tightly structured disc-shaped particle possessing a mean diameter of 101 +/- 0.4A and height of 54 +/- 0.7A. The absorption maxima remained the same in glutaraldehyde fixed material, and in dimer and trimer aggregates. Treatment with sodium dodecyl sulfate caused a breakdown into smaller units accompanied by a loss of the 563 nm peak. It is suggested that this absorption monomer is the in vivo functional species and comparable to the phycocyanin hexamer, but structurally distinguishable at the ultrastructural level. It has been calculated that about 35 phycobiliprotein molecules can be contained within each phycobilisome. There are 1.4 x 10(3) chlorophyll molecules per phycobilisome, but not contained within it.
INTELLIGENT VEHICLE NAVIGATION SYSTEM CONNECTED WITH INTERNET (2003)

International Telemetering Conference Proceedings / October 20-23, 2003 / Riviera Hotel and Convention Center, Las Vegas, Nevada
Effects of PCSK9 Inhibition With Alirocumab on Lipoprotein Metabolism in Healthy Humans (2016)

URL: http://www.clinicaltrials.gov. Unique identifier: NCT01959971.