gebhart/patrepeval huggingface dataset:
[The contribution of antioxidant enzymes in the cardioprotective effect of opioids during oxidative stress]. (2001)

Intravenous administration of peptide delta 2-opioid receptor agonists increased cardiac sarcolemma resistance to subsequent oxidative stress in the isolated perfused rat heart in vitro. delta-Opioid receptor stimulation prevented oxidative stress-induced accumulation of lipid peroxides in myocardium and inhibition of superoxide dismutase. The cardioprotective and "antioxidant" effects of delta-agonists were completely abolished by in vivo pretreatment with the delta-receptor antagonist ICI 174,864. mu-Opioid receptor activation in vivo did not protect the isolated myocardium from cardiotoxic action of activated oxygen species.
Giant backscattering peak in angle-resolved Andreev reflection (1995)

It is shown analytically and by numerical simulation that the angular distribution of Andreev reflection by a disordered normal-metal--superconductor junction has a narrow peak at the angle of incidence. The peak is higher than the well-known coherent backscattering peak in the normal state, by a large factor G/${\mathit{G}}_{0}$ (where G is the conductance of the junction and ${\mathit{G}}_{0}$=2${\mathit{e}}^{2}$/h). The enhanced backscattering can be detected by means of ballistic point contacts.
Tracking the World State with Recurrent Entity Networks (2016)

We introduce a new model, the Recurrent Entity Network (EntNet). It is equipped with a dynamic long-term memory which allows it to maintain and update a representation of the state of the world as it receives new data. For language understanding tasks, it can reason on-the-fly as it reads text, not just when it is required to answer a question or respond as is the case for a Memory Network (Sukhbaatar et al., 2015). Like a Neural Turing Machine or Differentiable Neural Computer (Graves et al., 2014; 2016) it maintains a fixed size memory and can learn to perform location and content-based read and write operations. However, unlike those models it has a simple parallel architecture in which several memory locations can be updated simultaneously. The EntNet sets a new state-of-the-art on the bAbI tasks, and is the first method to solve all the tasks in the 10k training examples setting. We also demonstrate that it can solve a reasoning task which requires a large number of supporting facts, which other methods are not able to solve, and can generalize past its training horizon. It can also be practically used on large scale datasets such as Children's Book Test, where it obtains competitive performance, reading the story in a single pass.
Model Structure for the Inflammatory Protein C5a (1985)

The complement cleavage product C5a is a potent stimulant of inflammatory processes; thus, inhibition of C5a activity is of therapeutic interest. The three-dimensional structure of the major portion of C5a was modeled from the homologous C3a crystal structure by comparative modeling techniques. The model shows that core residues of C5a are completely conserved, while external residues differ from C3a. Even though the amino-terminal 12 residues of C3a are disordered in the crystal, this sequence in C5a may form an amphipathic helix. The distribution of species sequence differences in the complete C5a structure suggests a possible receptor binding site.
Theory and Design of a Quadrature Analog-to-Information Converter for Energy-Efficient Wideband Spectrum Sensing (2014)

A flexible bandwidth, blind sub-Nyquist sampling approach referred to as the quadrature analog-to-information converter (QAIC) is proposed. The QAIC relaxes the analog front-end bandwidth requirements at the cost of some added complexity compared to the modulated wideband converter (MWC) for an overall improvement in sensitivity and energy consumption. An approach for detailed frequency domain analysis of the proposed system with linear impairments is developed. A process for selecting QAIC parameter values is illustrated through examples. The benefits of the QAIC are highlighted with cognitive radio use cases where a wide range of spectrum is observed at various resolution bandwidth settings. We demonstrate that the energy consumption of the QAIC is potentially two orders of magnitude lower than the swept-tuned spectrum analyzer (STSA) and an order of magnitude lower than the MWC. We also demonstrate that the QAIC significantly improves upon the sensitivity performance delivered by the MWC.
Synergistic Effect of TNF-α in Soluble VCAM-1-Induced Angiogenesis Through α4 Integrins (2003)

In our present study we focused on soluble VCAM-1 (sVCAM-1)/alpha(4) integrin-induced angiogenesis and found that this type of angiogenesis was mediated through p38 mitogen-activated protein kinase and focal adhesion kinase (FAK). HUVEC expressed both alpha(4) and beta(1) integrins, and it was reported that expression of alpha(4) integrin and its counterreceptor, sVCAM-1/VCAM-1, was enhanced in response to an inflammatory cytokine, TNF-alpha. In endothelial cells phosphorylation of p38 and FAK, but not that of extracellular signal-regulated kinase 1/2 was induced by sVCAM-1. Migration of endothelial cells was stimulated in response to sVCAM-1 at similar levels as those induced by vascular endothelial growth factor, and sVCAM-1-induced migration was almost completely blocked by neutralizing Ab against alpha(4) integrin, by either an inhibitor of p38 (SB203580), or by adenovirus containing FAK-related nonkinase. sVCAM-1 also induced the formation of blood vessels in Matrigel plug assay in vivo, and this neovascularization was blocked by SB203580 or neutralizing Ab against alpha(4) integrin. Moreover, we also confirmed that both TNF-alpha and sVCAM-1 could synergistically induce angiogenesis in the corneas of mice when each factor at used dose could not induce. This angiogenesis by TNF-alpha and sVCAM-1 was almost completely blocked by coadministration of SB203580 and also by neutralizing Ab against alpha(4) integrin. These results suggest that sVCAM-1/alpha(4) integrin induces angiogenesis through p38 and FAK signaling pathways.
The enduracidin biosynthetic gene cluster from Streptomyces fungicidicus (2006)

The biosynthetic gene cluster for the 17 aa peptide antibiotic enduracidin has been cloned and sequenced from Streptomyces fungicidicus ATCC 21013. The 84 kb gene cluster contains 25 ORFs and is located within a 116 kb genetic locus that was fully sequenced. Targeted disruption of non-ribosomal peptide synthetase (NRPS) genes in the cluster abolished enduracidin production and confirmed function. The cluster includes four genes, endA-D, encoding two-, seven-, eight- and one-module NRPSs, respectively, and includes unique modules for the incorporation of citrulline and enduracididine. The NRPS organization generally follows the collinearity principle, and starts with a condensation domain (C domain) similar to those found in other lipopeptide systems for the coupling of an acyl group to the starting amino acid. The sixth module of EndB, corresponding to Thr(8), is missing an adenylation domain (A domain) and this module is presumed to be loaded in trans by the single module protein EndD. The most striking feature of the NRPS organization is the lack of epimerization domains (E domains) in light of the fact that the product has seven d-amino acid residues. Sequence analysis reveals that C domains following modules corresponding to d-amino acids belong to a unique subset of C domains able to catalyse both epimerization and condensation reactions. Other genes directing lipid modification and activation, and formation of the non-proteinogenic amino acids 4-hydroxyphenylglycine and enduracididine are readily identified, as are genes possibly involved in regulation of antibiotic biosynthesis and export. These findings provide the basis to further genetically manipulate and improve lipodepsipeptide antibiotics via combinatorial and chemical methods.
Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes. (1994)

Extracts of insect cells infected with baculoviruses recombinant for the herpes simplex virus 1 (HSV-1)-encoded enzymes that are required for its replication can promote the rolling circle replication of circular plasmid templates. Replication is independent of a HSV-1 origin of replication (oris) or the HSV-1 origin binding protein and is inhibited by the origin binding protein when the plasmid contains oris. Replication is dependent on a complex composed of the HSV-1-encoded DNA polymerase and its processivity enhancing factor (the UL42 protein), ICP8 (the HSV-1-encoded single-strand DNA binding protein), and the HSV-1-encoded helicase-primase. The complex can be purified by size-exclusion and anion-exchange chromatography.
Beneficial effects of naloxone in a patient with intestinal pseudoobstruction. (1985)

A 15-day course of Naloxone treatment was given to a patient with intestinal pseudoobstruction who had previously undergone subtotal colectomy with terminal ileostomy for invalidating constipation. The effects of the drug were assessed according to symptoms, by recording the myoelectric activity of the stomach, and by measuring gastric emptying of a radiolabeled solid-liquid meal and the intestinal transit time of radiopaque markers. All tests were performed 1) at baseline; 2) after 2 wk with Naloxone 1.6 mg subcutaneous per day; and 3) after 8 days of placebo. Results showed that before treatment gastric emptying of solids was delayed, emptying of liquids was normal, myoelectric activity of the stomach was normal, small intestinal transit time of radiopaque markers was considerably increased while ileal output was markedly decreased. After Naloxone, gastric emptying of solids was markedly accelerated, emptying of liquids remained normal, gastric electrical spiking activity increased, small intestinal transit time strikingly decreased, and ileal output increased. After placebo, a tendency to return to pretreatment values was observed. This observation suggests that Naloxone may be helpful in the treatment of some patients with intestinal pseudoobstruction.
The Stromal Cell–Derived Factor-1 Chemokine Is a Potent Platelet Agonist Highly Expressed in Atherosclerotic Plaques (2000)

Chemokines are chemotactic cytokines that activate and direct the migration of leukocytes. However, their role in modulating platelet function has not been shown. We studied the direct effect of chemokines on human platelets and found that of the 16 tested only stromal cell-derived factor (SDF)-1 induced platelet aggregation, accompanied by a rise in intracellular calcium. Platelets expressed the SDF-1 receptor, CXCR4, and an antibody to CXCR4 and pertussis toxin inhibited SDF-1-induced platelet aggregation, confirming that this effect is mediated through CXCR4, a Galphai-coupled receptor. SDF-1-induced platelet aggregation was also inhibited by wortmannin, LY294002, and genistein, suggesting that phosphatidylinositol 3-kinase and tyrosine kinase are likely involved in SDF-1-induced platelet aggregation. Because chemokines are produced from multiple vascular cells and atherosclerotic vessels are prone to develop platelet-rich thrombi, we examined the expression of SDF-1 in human atheroma. SDF-1 protein was highly expressed in smooth muscle cells, endothelial cells, and macrophages in human atherosclerotic plaques but not in normal vessels. Our studies demonstrate a direct effect of a chemokine in inducing platelet activation and suggest a role for SDF-1 in the pathogenesis of atherosclerosis and thrombo-occlusive diseases.
Intracellular Targeting with Low pH-triggered Bispecific Antibodies (1997)

Bispecific antibodies were designed to deliver a reversibly bound ligand into target cells and then spontaneously release it upon passage into acidified vesicles. These reagents were assembled by coupling monoclonal antibodies that recognize acid-sensitive epitopes on diphtheria toxin to cell type-specific monoclonal antibodies. The dual binding capacity of the bispecific antibodies was confirmed by delivery of 125I-diphtheria toxin to target molecules present on intact cells. Bispecific antibodies directed against transferrin receptors on human cells were loaded with toxin and tested for cytotoxicity. The mutant diphtheria toxins CRM107 and CRM45 were used since their inability to bind cell receptors renders them ordinarily nontoxic. Their full cytotoxic potential, however, was restored via bispecific antibody-mediated delivery and release within low pH intracellular vesicles. Cytotoxicity was shown to be specific by blocking receptor sites and to be acidification-dependent by protection using NH4Cl to raise endosomal pH. Kinetics for inhibition of cellular protein synthesis was identical for native diphtheria toxin and the bispecific antibody. CRM107 combination. The rate of inhibition (t1/2 = 20 min) indicated that release of CRM107 from the antibody combining site was fast, and its toxic action was unimpeded by this delivery mechanism.
Efferent Vagal Fibre Stimulation Blunts Nuclear Factor-κB Activation and Protects Against Hypovolemic Hemorrhagic Shock (2003)

Our results show a parasympathetic inhibition of NF-kappaB by which the brain opposes NF-kappaB activation in the liver and modulates the inflammatory response during acute hypovolemic hemorrhagic shock.
Heat-induced blebbing and vesiculation of the outer membrane of Escherichia coli (1982)

Thermal damage to the outer membrane of Escherichia coli W3110 was studied. When E. coli cells were heated at 55 degrees C in 50 mM Tris-hydrochloride buffer at pH 8.0, surface blebs were formed on the cell envelope, mainly at the septa of dividing cells. Membrane lipids were released from the cells during the heating period, and part of the released lipids formed vesicle-like structures from the membrane. This vesicle fraction had a lipopolysaccharide to phospholipid ratio similar to that of the outer membrane of intact cells, whereas it had a lower content of protein than the isolated outer membrane. After heating bacterial cells at 55 degrees C for 30 min, the resulting leakage from the cells of a periplasmic enzyme, alkaline phosphatase, amounted to 52% of the total activity, whereas no release of a cytoplasmic enzyme, glucose-6-phosphate dehydrogenase, was detected. The results obtained suggest that surface blebs formed by heat treatment almost completely consist of the outer membrane and that the blebs may be gradually released from the cell surface into the heating menstruum to partially form vesicles.
FurinDB: A Database of 20-Residue Furin Cleavage Site Motifs, Substrates and Their Associated Drugs (2011)

FurinDB (freely available online at http://www.nuolan.net/substrates.html) is a database of furin substrates. This database includes experimentally verified furin cleavage sites, substrates, species, experimental methods, original publications of experiments and associated drugs targeting furin substrates. The current database release contains 126 furin cleavage sites from three species: mammals, bacteria and viruses. A main feature of this database is that all furin cleavage sites are recorded as a 20-residue motif, including one core region (eight amino acids, P6-P2') and two flanking solvent accessible regions (eight amino acids, P7-P14, and four amino acids, P3'-P6'), that represent our current understanding of the molecular biology of furin cleavage. This database is important for understanding the molecular evolution and relationships between sequence motifs, 3D structures, cellular functions and physical properties required by furin for cleavage, and for elucidating the molecular mechanisms and the progression of furin cleavage associated human diseases, including pathogenic infections, neurological disorders, tumorigenesis, tumor invasion, angiogenesis, and metastasis. FurinDB database will be a solid addition to the publicly available infrastructure for scientists in the field of molecular biology.
Antioxidant effect of natural phenols on olive oil (1991)

Abstract The total polar fraction and individual phenols present in virgin olive oil were tested for their antioxidant effect in refined olive oil. Hydroxytyrosol and caffeic acid showed protection factors greater than BHT. Protocatechuic and syringic acid were also found to have antioxidant activity. Tyrosol, p‐hydroxyphenylacetic acid, o‐coumaric acid, p‐coumaric acid, p‐hydroxybenzoic acid and vanillic acid had very little or no effect, and their contribution to the stability of the oil is negligible.
The Troyer Syndrome (1967)

FAMILIAL spastic paraplegia was first reported by Seeligmüller,<sup>1</sup>and many additional cases have since been reported (see reviews by Rhein,<sup>2</sup>Paskind and Stone,<sup>3</sup>and Bell and Carmichael.<sup>4</sup>Although the diagnosis is commonly made in patients with evidence of pyramidal tract disease beginning in the lower extremities, the disease is frequently more extensive and may progress to involve the arms as well as cranial nerves. Some difference of opinion exists<sup>4,5</sup>regarding the full range of clinical manifestations of spastic paraplegia, but families have been reported with mental defect, optic atrophy, nystagmus, ataxia, seizures, rigidity, muscle atrophy, kyphosis, pes cavus, and cardiac conduction defects. The reported families probably comprise a heterogeneous group of disorders with somewhat similar clinical features, and as yet no pathological or biochemical distinctions are possible. On genetic grounds, however, at least three types must exist: one genetic defect produces dominant spastic paraplegia,<sup>4</sup>
N‐Heterocyclic Carbene Organocatalysts for Dehydrogenative Coupling of Silanes and Hydroxyl Compounds (2013)

Go organic! N-Heterocyclic carbene (NHC) 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene (IiPr) has been found to be an efficient and selective catalyst for the dehydrogenative coupling of a wide range of silanes and hydroxyl groups to form SiO bonds under mild and solvent-free conditions (see scheme). Mechanistic studies indicated that the activation of hydroxyl groups by the NHC is the most plausible initial step for the process. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Retargeting interleukin 13 for radioimmunodetection and radioimmunotherapy of human high-grade gliomas. (1999)

A vast majority of patients with glioblastoma multiforme (GBM), a high-grade glioma, overexpress abundant amounts of a receptor for interleukin (IL)-13 in situ. This receptor is more restrictive because it is IL-4-independent and therefore differs from the IL-13/4 signaling receptor of normal tissue that is shared with IL-4. We previously identified one of the sites on the human IL (hIL)-13 molecule that is important for its interaction with the IL-13/4 receptor, a residue of glutamic acid at position 13. In this study, we mutated the cytokine and produced hIL-13.E13Y, in which the glutamic acid was substituted by tyrosine. This additional tyrosine residue was therefore strategically located within the region of IL-13 interaction with the signaling physiological receptor. hIL-13.E13Y did not transduce signals through the IL-13/4 receptor, whereas its interaction with the more restrictive, GBM-associated receptor remained intact. The mutated hIL-13 could be readily radiolabeled. Radiolabeled hIL-13.E13Y produced specific autoradiographic images of human GBM specimens. We demonstrate an effective way to redirect hIL-13 to its more restrictive receptor found in high-grade gliomas by mutagenizing the cytokine, and, concomitantly, we equipped hIL-13 with an additional tyrosine residue for higher specific activity radiolabeling.
Sequential Cross-Coupling of 1,4-Bissilylbutadienes:  Synthesis of Unsymmetrical 1,4-Disubstituted 1,3-Butadienes (2005)

A mild and general and stereospecific cross-coupling reaction of unsymmetrical 1,4-bissilyl-1,3-butadienes has been accomplished. By the use of either a benzyldimethylsilyl or 2-thienyldimethylsilyl unit at one end of the dienylsilanol, a selective cross-coupling could be effected under mildly basic conditions (KOTMS) to afford 4-aryl-1,3-dienylsilanes in excellent yield for a wide range of aryl and alkenyl coupling partners. The second cross-coupling could be effected cleanly by the action of TBAF with electron-rich or electron-poor halides. The sequential process could be telescoped into a "one pot" procedure with overall excellent yields of the unsymmetrical 1,4-diaryl-1,3-butadienes.
Effect of 2,5‐norbornadiene on abscission and ethylene production in citrus leaf explants (1985)

Citrus ( Citrus sinensis L. Osbeck) leaf explants completely abscise within 48 h when exposed to saturating amounts of ethylene at 25°C. When 2,5‐norbornadiene was added, 2000 μl 1 −1 reduced abscission of explants also exposed to 2 μl 1 −1 of ethylene to the level of the control, and 8000 μl 1 −1 reduced abscission in explants exposed to 10 μl 1 −1 of ethylene to the level of the control, but abscission was complete when 1 000 μl 1 −1 of ethylene was used in the presence of 8 000 μl 1 −1 of 2,5‐norbornadiene. When explants were exposed to 2 μl 1 −1 of ethylene, 2000 μl 1 −1 of 2,5‐norbornadiene prevented abscission if applied up to 10 h after exposure to ethylene. After 18 h, applied 2,5‐norbornadiene had little effect on abscission at 48 h. A Lineweaver‐Burk plot gave a 1/2 maximum value of 0.12 μl 1 −1 for ethylene on abscission, 2,5‐Norbornadiene gave competitive kinetics with respect to ethylene with a K 1 value of approximately 120 μl 1 −1 of 2,5‐norbornadiene. The presence of 2,5norbornadiene stimulated ethylene production, which progressively increased as the 2,5‐norbornadiene concentration was increased from 250 to 8 000 μl 1 −1 2,5‐Norbornadiene also suppressed the induction of cellulase and polygalacturonase by ethylene. Together, 2,5‐norbornadiene and 2,4‐dichlorophenoxyacetic acid were more effective than either alone in reducing abscission. 2,5‐Norbornadiene also was effective in preventing the reduction of indole‐3‐acetic acid transport induced by ethylene.
High Seroprevalence of Human Metapneumovirus among Young Children in Israel (2003)

Exposure to the newly discovered human metapneumovirus (HMPV) during the first 2 years of life was studied by longitudinal serological analysis in 40 healthy children in southern Israel. The seropositivity rate decreased to a minimum by age 13 months and increased to 52% by age 24 months. Evidence of new infection was detected in 13%, 23%, and 55% of children by ages 7, 13, and 24 months, respectively. The high exposure rates suggest that HMPV may be an important cause of community-acquired respiratory-tract infections in young children.
Cross-sectional evaluation of YKL-40 serum concentrations in patients with systemic sclerosis. Relationship with clinical and serological aspects of disease. (2003)

This preliminary study shows that YKL-40 serum levels are increased in SSc and that they are correlated with sIL-2Ra and joint involvement, suggesting a relationship with cartilage and/or fibroblast activity.