gebhart/patrepeval huggingface dataset:
Functional Cloning of IGFBP-3 From Human Microvascular Endothelial Cells Reveals its Novel Role in Promoting Proliferation of Primitive CD34+CD38− Hematopoietic Cells In Vitro (2003)

Ex vivo expansion of hematopoietic stem cells (HSCs) has been investigated as a means of enhancing engraftment of transplantation therapies, but current ex vivo expansion methods typically result in a loss of functional stem cell activity. Factors that can selectively expand human HSCs remain elusive. Recently we have isolated three functionally distinct human brain microvascular endothelial cells (HBMVECs) that differ greatly in their ability to support in vitro proliferation of human umbilical cord blood (UBC) CD34+CD38-cells. Using these distinct HBMVEC populations, we have devised a cell-based functional cloning assay to identify a molecule(s) capable of facilitating expansion of HSCs in vitro. A gene encoded for IGFBP-3 (insulin-like growth factor binding protein-3) has been identified. IGFBP-3 mRNA and protein are differentially expressed in distinct HBMVEC populations. In vitro cell proliferation assay and CD34+CD38- immunophenotype analysis showed that the addition of an exogenous IGFBP-3 to cultures of purified CD34+/-CD38-Lin- cells (CD2/CD3/CD14/CD16/CD19/CD24/CD56/CD66b/GlyA depleted) enhanced proliferation of primitive hematopoietic cells with CD34+CD38- phenotype, suggesting that IGFBP-3 is capable of expanding primitive human blood cells. These expanded primitive blood cells were illustrated to maintain ability to generate functional progenitors. IGFBP-3 belongs to a family of high-affinity IGFBPs, which binds to IGFs and modulates their actions. IGFBP-3 appears to have intrinsic bioactivity that is independent of IGF binding. We are currently exploring the underlying mechanism by which IGFBP-3 modulates proliferation of primitive hematopoietic cells, and the potential of IGFBP-3 to expand pluripotent human repopulating cells capable of hematopoietic reconstitution of irradiated NOD/SCID recipients.
<title>Video indexing: an approach based on moving object and track</title> (1993)

With the progression of multimedia technology and the trend of visualization of man-machine interface, video data will become a kind of fundamental resource of data as general as text and graphics. When the data size is large, retrieving data will become a time-consuming process. Much research has been done to facilitate the retrieval of data, e.g., indexes in a database. Similarly, the video data also encounter the same problem. It is very difficult to generate video indexes automatically, because the index is application-dependent and only the user knows what indexes he actually needs. In this paper a mechanism for video data indexing is proposed which is based on the concept of objects and object motion with interactive annotation. This mechanisms provides an efficient method to access video substance without browsing the whole video data. A motion representation for the track of a moving object is presented. The access methods of video queries are introduced. A prototype of the video indexing system is implemented.
Reliability Modeling, Analysis and Prediction of Wireless Mobile Communications (2014)

The future Fifth Generation (5G) mobile cellular networks that are currently in research phase today enable broad range of services/applications beyond classical mobile communications. One key enabler for Ultra-Reliable services to be integrated into mobile networks is the Reliability of transmission success of a given data packet. This is harder mainly owing to the time-dependent effective link qualities of the communicating devices. However, successful indication of the availability of the instantaneous link quality (e.g., by the device) would allow opportunistic access of ultra reliable services/applications when the link conditions are fair enough. This paper introduces a framework for modeling, predicting and analyzing the theoretical reliability of the wireless link based on factors such as fading, mobility, interference etc. The analysis and prediction is based on the part stress method [1] by assuming time dependent factors as elements/components and their respective Transmission Times To Failure (TTTF). The proposed framework also supports other reliability analysis techniques such as Fault Tree Analysis [2] and Accelerated testing [3] of wireless systems and to improve the components.
Burkitt's Lymphoma: Clinicopathologic Features and Differential Diagnosis (2006)

Burkitt's lymphoma is a highly aggressive lymphoma identified and described in the last century by Denis Burkitt in Africa, in areas endemic for malaria. Since its description in African children, it has been recognized outside areas with endemic malaria, frequently also in children as well as among individuals with an underlying immunodeficiency. Since its initial designation as Burkitt's lymphoma, this type of lymphoma and lymphomas closely resembling it have received a variety of names in different classifications of lymphomas and leukemias: undifferentiated lymphoma, Burkitt's and non-Burkitt's type in the modified Rappaport Classification, malignant lymphoma, small non-cleaved cell, Burkitt's type in the Working Formulation, Burkitt's lymphoma and high-grade B-cell lymphoma, Burkitt-like in the REAL Classification, and acute lymphoblastic leukemia, L3 type in the FAB Classification. With the publication of the WHO Classification of Haematopoietic and Lymphoid Tumors, the nomenclature of this lymphoma has come full circle, and it is once again known simply as Burkitt's lymphoma. In recent years, efforts have focused on improving therapy for this rapidly proliferating neoplasm while minimizing, to the extent possible, treatment-associated toxicity. These efforts have led to the development of high-intensity, short-duration combination chemotherapy that has proven extremely effective for a high proportion of Burkitt's lymphoma patients. The differential diagnosis of Burkitt's lymphoma is broad, and precise diagnosis based on histologic, immunophenotypic, and genetic features remains the critical first step in planning appropriate therapy.
Personal Brokerage of Web Service Access (2007)

Network smart-card technology can potentially meet the growing need for secure, flexible Web services. This technology allows building personal identity brokerage solutions that bridge independent Web services into new simple sign-on systems. The network smart card can help bring about a new generation of personal authentication services.
The overgrown hematopoietic organs-31 tumor suppressor gene of Drosophila encodes an Importin-like protein accumulating in the nucleus at the onset of mitosis. (1995)

The tumor suppressor gene overgrown hematopoietic organs-31 (oho31) of Drosophila encodes a protein with extensive homology to the Importin protein of Xenopus (50% identity), the related yeast SRP1 protein, and the mammalian hSRP1 and RCH1 proteins. A strong reduction in the expression of oho31 by a P element inserted in the 5' untranslated region of the oho31 transcript or a complete inactivation of oho31 by imprecise P element excision leads to malignant development of the hematopoietic organs and the genital disc, as shown by their growth autonomy in transplantation assays. We have cloned the oho31 gene of Drosophila melanogaster and determined its nucleotide sequence. The gene encodes a phosphoprotein of 522 amino acids made of three domains: a central hydrophobic domain of eight repeats of 42-44 amino acids each, displaying similarity to the arm motif found in junctional and nucleopore complex proteins, and flanked by two hydrophilic NH2- and COOH-terminal domains. Immunostaining revealed that the OHO31 protein is supplied maternally and rapidly degraded during the first 13 nuclear divisions. Thereafter, the OHO31 protein is predominantly expressed, albeit at reduced levels, in proliferating tissues. During the interphase of early embryonic cell cycles, the OHO31 protein is present in the cytoplasm and massively accumulates in the nucleus at the onset of mitosis in late interphase and prophase. The nuclear import of OHO31 is, however, less pronounced during later developmental stages. These results suggest that, similar to Importin, OHO31 may act as a cytosolic factor in nuclear transport. Moreover, the cell cycle-dependent accumulation of OHO31 in the nucleus indicates that this protein may be required for critical nuclear reactions occurring at the onset of mitosis.
Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins. (1988)

The human platelet-derived growth factor (PDGF) A-chain locus was characterized by restriction endonuclease analysis, and the nucleotide sequence of its exons was determined. Seven exons were identified, spanning approximately 22 kilobase pairs of genomic DNA. Alternative exon usage, identified by cDNA cloning, occurs in a human glioblastoma cell line and may give rise to two types of A-chain precursors with different C termini. The exon-intron arrangement was similar to that of the PDGF B-chain/sis locus and seemed to divide the precursor proteins into functional domains. Southern blot analysis of genomic DNA showed that a single PDGF A-chain gene was present in the human genome.
Frameshift mutations of autophagy‐related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability (2008)

Mounting evidence indicates that alterations of autophagy processes are directly involved in the development of many human diseases, including cancers. Autophagy-related gene (ATG) products are main players in the autophagy process. In humans there are 16 known ATG genes, of which four (ATG2B, ATG5, ATG9B and ATG12) have mononucleotide repeats with seven or more nucleotides. Frameshift mutations of genes with mononucleotide repeats are features of cancers with microsatellite instability (MSI). It is not known whether ATG genes with mononucleotide repeats are altered by frameshift mutations in gastric and colorectal carcinomas with MSI. For this, we analysed the mononecleotide repeats in ATG2B, ATG5, ATG9B and ATG12 in 32 gastric carcinomas with high MSI (MSI-H), 13 gastric carcinomas with low MSI (MSI-L), 43 colorectal carcinomas with MSI-H and 15 colorectal carcinomas with MSI-L by a single-strand conformation polymorphism (SSCP) analysis. We found ATG2B, ATG5, ATG9B and ATG12 mutations in 10, 2, 13 and 0 cancers, respectively. The mutations were detected in MSI-H cancers but not in MSI-L cancers. Gastric and colorectal cancers with MSI-H harboured one or more ATG mutations in 28.1% and 27.9%, respectively. Our data indicate that frameshift mutations in ATG genes with mononucleotide repeats are common in gastric and colorectal carcinomas with MSI-H, and suggest that these mutations may contribute to cancer development by deregulating the autophagy process.
A Few to Flip the Angiogenic Switch (2008)

A relatively small number of endothelial progenitor cells have a profound effect on tumor growth and metastasis.
Photochemical generation of stable fluorescent compounds (photofluorescence) (1973)

Abstract
Reduction of partial‐volume artifacts with zero‐filled interpolation in three‐dimensional MR angiography (1994)

Partial-volume artifacts reduce vessel contrast and continuity (especially in small vessels) in magnetic resonance (MR) angiography. The authors applied zero-filled (band-limited) interpolation to three-dimensional (3D) MR angiograms to reduce partial-volume artifacts. They demonstrated that zero-filled interpolation can also be implemented by means of voxel shifting in real space. Voxel-shifted interpolation is much less computer memory intensive than conventional zero-filled interpolation. They numerically simulated the contrast loss due to partial-volume artifacts and contrast recovery obtained with zero-filled interpolation. Zero-filled interpolation in all three orthogonal directions was applied to 3D MR angiography data sets from 29 human studies. These studies were obtained with the three commonly used 3D MR angiography techniques: 3D time of flight, multislab 3D time of flight, and 3D phase contrast. A substantial improvement in vessel contrast and vessel continuity was observed in all cases.
Calculating the Statistical Significance of Changes in Pathway Activity From Gene Expression Data (2004)

We present a statistical approach to scoring changes in activity of metabolic pathways from gene expression data. The method identifies the biologically relevant pathways with corresponding statistical significance. Based on gene expression data alone, only local structures of genetic networks can be recovered. Instead of inferring such a network, we propose a hypothesis-based approach. We use given knowledge about biological networks to improve sensitivity and interpretability of findings from microarray experiments. Recently introduced methods test if members of predefined gene sets are enriched in a list of top-ranked genes in a microarray study. We improve this approach by defining scores that depend on all members of the gene set and that also take pairwise co-regulation of these genes into account. We calculate the significance of co-regulation of gene sets with a nonparametric permutation test. On two data sets the method is validated and its biological relevance is discussed. It turns out that useful measures for co-regulation of genes in a pathway can be identified adaptively. We refine our method in two aspects specific to pathways. First, to overcome the ambiguity of enzyme-to-gene mappings for a fixed pathway, we introduce algorithms for selecting the best fitting gene for a specific enzyme in a specific condition. In selected cases, functional assignment of genes to pathways is feasible. Second, the sensitivity of detecting relevant pathways is improved by integrating information about pathway topology. The distance of two enzymes is measured by the number of reactions needed to connect them, and enzyme pairs with a smaller distance receive a higher weight in the score calculation.
Automatic Recording Apparatus for Use in Chromatography of Amino Acids (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAutomatic Recording Apparatus for Use in Chromatography of Amino AcidsD. H. Spackman, W. H. Stein, and Stanford. MooreCite this: Anal. Chem. 1958, 30, 7, 1190–1206Publication Date (Print):July 1, 1958Publication History Published online1 May 2002Published inissue 1 July 1958https://pubs.acs.org/doi/10.1021/ac60139a006https://doi.org/10.1021/ac60139a006research-articleACS PublicationsRequest reuse permissionsArticle Views3221Altmetric-Citations7629LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
ROOM-TEMPERATURE 2-µm Ho : YAG AND 3-µm Er : YAG LASERS (1987)

We demonstrate fundamental laser properties fo Cr-sensitized Tm, Ho, Er-garnets like YAG, YSAG and YSGG. In Cr, Tm, Ho : doped YAG, YSAG and YSGG crystals cw-lasing is achieved with efficiencies up to 41 %. Laser operation was also obtained under flashlamp pumping. Even Cr, Er : and Cr, Er, Ho : doped YAG and YSGG crystals oscillate in a cw-mode. All these lasers are working efficiently at room-temperature.
The Connective Tissue Growth Factor/Cysteine- Rich 61/Nephroblastoma Overexpressed (CCN) Family* (1999)

I. Introduction II. The Modular Structure of the Connective Tissue Growth Factor/Cysteine-Rich 61/Nephroblastoma Overexpressed (CCN) Family Members III. Connective Tissue Growth Factor (CTGF) A. Discovery B. Structure of the CTGF gene and protein C. CTGF mRNA production D. CTGF production, secretion, and processing E. CTGF-heparin interactions F. Biological properties of CTGF G. Mechanism of action of CTGF IV. Cyr61 A. Discovery of cyr61 B. Structure of the cyr61 gene and protein C. Cyr61 mRNA production D. Cyr61 production and secretion E. Biological properties of cyr61 F. Mechanism of action of cyr61 V. Nov A. Discovery and structure of nov B. Nov mRNA production C. Biological properties of nov VI. Elm1/WISP-1 VII. Heparin-Induced CCN-Like Protein (HICP)/rCop-1/CTGF-3/WISP-2 VIII. WISP-3 IX. Other CCN-Like Molecules X. Regulation of Cellular Functions by the CCN Family A. Cell cycle control B. Cell adhesion and migration C. Extracellular matrix (ECM) production XI. Biological Processes Involving the CCN...
Efficient management for large-scale flash-memory storage systems with resource conservation (2005)

Many existing approaches on flash-memory management are based on RAM-resident tables in which one single granularity size is used for both address translation and space management. As high-capacity flash memory is becoming more affordable than ever, the dilemma of how to manage the RAM space or how to improve the access performance is emerging for many vendors. In this article, we propose a tree-based management scheme which adopts multiple granularities in flash-memory management. Our objective is to not only reduce the run-time RAM footprint but also manage the write workload, due to housekeeping. The proposed method was evaluated under realistic workloads, where significant advantages over existing approaches were observed, in terms of the RAM space, access performance, and flash-memory lifetime.
The role of sulfur-containing amino acids in superoxide production and modification of low density lipoprotein by arterial smooth muscle cells. (1987)

Extracellular superoxide (O2-.) was detected in cultures of monkey arterial smooth muscle cells as measured by the superoxide dismutase-inhibitable reduction of cytochrome c and acetylated cytochrome c. Reduction of cytochrome c by these cells required L-cystine in the incubation medium. A variety of other sulfur-containing amino acids, including D-cystine, L-cystathionine, L-methionine, and djenkolic acid did not support O2-. generation when present at concentrations equimolar to L-cystine. At millimolar concentrations, the chelators EDTA and diethylene triamine penta-acetic acid inhibited O2-. production by smooth muscle cells. This effect was maximal when the chelator was present at the same concentration as the sum of the Ca2+ and Mg2+ in the medium, suggesting a role for these cations in O2-. generation by cells. Modification of low density lipoprotein (LDL) by arterial smooth muscle cells, as assessed by changes in lipid peroxide content, mobility on agarose gel electrophoresis, and apoprotein B fragmentation, was also L-cystine-dependent. LDL modification also required micromolar concentrations of the transition metal ion Cu(II) or Fe(III) and was inhibited by superoxide dismutase. LDL modified by smooth muscle cells in the presence of L-cystine and Cu(II) was taken up and degraded less well than native LDL by human skin fibroblasts, suggesting that recognition by the LDL receptor was lost. In contrast, LDL modified by smooth muscle cells was taken up and degraded to a greater degree than native LDL by mouse peritoneal macrophages, consistent with recognition by the scavenger receptor. These results indicate that monkey arterial smooth muscle cells produce O2-. and modify LDL by an L-cystine-dependent process. This may involve reduction of cystine to a thiol, possibly cysteine or a cysteine-containing peptide such as glutathione. Sulfur-containing amino acids may play a role in atherogenesis by supporting cell-mediated generation of reactive oxygen species and modification of lipoprotein to a form recognized by the scavenger receptor.
In vitro Conversion of the Carbohydrate Moiety of Fungal Glycoproteins to Mammalian‐type Oligosaccharides (1997)

To investigate the potential of filamentous fungi to synthesize N-glycans that are convertible to a mammalian type, in vitro glycosylation assays were performed. Recombinant human N-acetylglucosaminyltransferase I, human beta-1,4-galactosyltransferase and rat alpha-2,6-sialyltransferase were successively used to mimic part of the mammalian glycosylation synthesis pathway. High-mannose carbohydrates on Trichoderma reesei cellobiohydrolase I were converted to a hybrid mammalian-type structure. Successful modification varied markedly with the strain of T. reesei used to produce cellobiohydrolase I. In vitro pretreatment of fungal glycoproteins with Aspergillus saitoi alpha-1,2-mannosidase improved subsequent hybrid formation. It was, however, not possible to trim all fungal oligosaccharides to an acceptor substrate for mammalian glycosyltransferases. With T. reesei RUTC 30, capping glucose residues and phosphate groups were shown to be responsible for this lack of trimming. N-glycan processing in T. reesei apparently involves different steps, including alpha-1,2-mannosidase trimmings, and thus resembles the first mammalian glycosylation processes. The alpha-1,2-mannosidase trimming steps can be exploited for further in vitro and/or in vivo synthesis of complex oligosaccharides on (heterologous) glycoproteins from filamentous fungi.
Profound Enhancement of T Cell Activation Mediated by the Interaction Between the TCR and the D3 Domain of CD4 (1999)

Abstract CD4 plays an important role in the activation and development of CD4+ T cells. This is mediated via its bivalent interaction with MHC class II molecules and the TCR:CD3 complex through p56lck. Recent studies have implicated a third site of interaction between the membrane-proximal extracellular domains of CD4 and the TCR. Due to these multiple interactions, direct evidence for the functional importance of this extracellular association has remained elusive. Furthermore, the residues that mediate this interaction are unknown. In this study, we analyzed the function of 61 CD4 mutants. Alanine substitution of just 2 residues, either Q114/F182 or F182/F201, which are partially buried and located close to the D2/D3 interface, completely abrogated CD4 function. Direct evidence for the functional importance of TCR:CD4.D3 interaction was obtained using an anti-CD3fos:anti-CD4jun-bispecific Ab. Surprisingly, it induced strong T cell activation in hybridomas transfected with cytoplasmic-tailless CD4, despite the lack of association with either p56lck or MHC class II molecules. However, this effect was completely abrogated with the CD4 mutants Q114A/F182A or F182A/F201A. These data demonstrate that TCR:CD4.D3 interaction can have a profound effect on T cell activation and obviates the need for receptor oligomerization.
A novel malaria protein, Pfs28, and Pfs25 are genetically linked and synergistic as falciparum malaria transmission-blocking vaccines (1997)

Antibodies to Pfs28 block Plasmodium falciparum transmission and when combined with antibodies to Pfs25 provide synergy in blocking transmission. Pfs28 and Pfs25 are immunogenic, have limited antigenic diversity, and are structurally similar and genetically linked on chromosome 10. Pfs28 may prove a useful addition to Pfs25 in an effective transmission-blocking vaccine.
Rapid Clearance of Psoriatic Skin Lesions Induced by Topical Cyclopamine (2004)

An effective treatment for psoriasis is described, consistent with intervention in a proximal/key pathogenic event.