gebhart/patrepeval huggingface dataset:
Role of kinins and nitric oxide in the effects of angiotensin converting enzyme inhibitors on neointima formation. (1993)

Marked neointima formation occurs after balloon injury to the intima of rat arteries. Angiotensin II has been implicated as a growth factor in this process, since angiotensin converting enzyme (ACE) inhibitors block neointima formation after injury. However, ACE is an important kininase, and its inhibitors may act in part by a kinin-mediated mechanism. Kinins are also known to stimulate synthesis of endothelium-derived relaxing factor/nitric oxide (EDRF/NO) and prostacyclin, both of which have antigrowth effects. To determine whether the effect of ACE inhibitors on neointima formation is due to blockade of angiotensin II synthesis alone and/or inhibition of kinin inactivation, we followed two approaches. First, we compared the inhibition of neointima formation induced by the AT1-type angiotensin II receptor antagonist losartan with that caused by the ACE inhibitor ramipril. We also studied whether a kinin receptor antagonist, Hoe 140, blocks the effect of two different ACE inhibitors, ramipril and enalapril, on neointima formation. In addition, we studied whether the effect of ramipril is blocked by an NO synthesis inhibitor, N omega-nitro-L-arginine-methyl ester (L-NAME). Although both ramipril and losartan significantly reduced neointima formation, ramipril had a more marked effect (p < 0.05 for ramipril versus losartan). The kinin antagonist Hoe 140 reduced the inhibitory effect of ramipril and enalapril by 73% and 62%, respectively. The remaining effect of the ACE inhibitors was now similar to that of losartan. Inhibition of neointima formation by ramipril was also blocked by the NO synthesis inhibitor L-NAME.(ABSTRACT TRUNCATED AT 250 WORDS)
Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-ligated cDNA templates (1992)

Japanese encephalitis virus (JEV) is a positive-stranded enveloped RNA virus that belongs to the family Flaviviridae. Genomic JEV RNA is approximately 11 kb long and encodes 10 proteins, 3 structural and 7 nonstructural. A full-length cDNA copy of the JEV genome was constructed by in vitro ligation of two cDNA fragments which encode the 5' (nucleotide positions 1 to 5576) and 3' (nucleotide positions 5577 to 10976) halves of the genome. T7 RNA polymerase transcripts of the ligated full-length cDNA template were infectious when transfected into BHK-21 cells. To identify the recombinant virus, a silent mutation was introduced into the clone encoding the 3' half of the genome, which abolished an XbaI site at nucleotide position 9131. Virus recovered by transfection with the transcripts contained this silent mutation, confirming its identity. Recombinant and parent viruses were identical with respect to growth and plaque production in BHK-21 cells, envelope protein expression in C6/36 cells, and neurovirulence and immunogenicity in mice. Repeated attempts to obtain infectious RNA by transcription from full-length JEV genome cDNA templates cloned into plasmid vectors were unsuccessful. Synthesis of infectious JEV RNA from in vitro-ligated JEV cDNA templates will be useful for molecular and genetic studies of flavivirus replication and virulence.
Phase II clinical studies of denileukin diftitox diphtheria toxin fusion protein in patients with previously treated chronic lymphocytic leukemia (2006)

Follow-up studies will be required to identify predictors of response that may improve the response rate to denileukin diftitox in patients with CLL.
Molecular anatomy of the transcription complex of Escherichia coli during initiation. (1992)

Transcription is the foremost event in gene expression in which the enzyme RNA polymerase copies the genetic information from DNA to RNA. Much of our understanding of this process have come from studies carried out in Escherichia coli. A faithful and efficient transcription machinery of E. coli can be reconstituted in vitro with purified RNA polymerase and promoter-containing DNA. It is generally believed that in E. coli and most other organisms, the control of gene expression lies with the initiation of transcription. In this review, an attempt has been made to understand the mechanistic details of the initiation of transcription from the structural point of view of the promoter and the RNA polymerase. Allosteric nature of the enzyme has also been discussed at the end.
Structural Studies of a Family of High Affinity Ligands for GPIIb/IIIa (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural Studies of a Family of High Affinity Ligands for GPIIb/IIIaAlvin C. Bach II, Charles J. Eyermann, John D. Gross, Michael J. Bower, Richard L. Harlow, Patrica C. Weber, and William F. DeGradoCite this: J. Am. Chem. Soc. 1994, 116, 8, 3207–3219Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/ja00087a006https://doi.org/10.1021/ja00087a006research-articleACS PublicationsRequest reuse permissionsArticle Views270Altmetric-Citations114LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
A Murine Model of falciparum-Malaria by In Vivo Selection of Competent Strains in Non-Myelodepleted Mice Engrafted with Human Erythrocytes (2008)

To counter the global threat caused by Plasmodium falciparum malaria, new drugs and vaccines are urgently needed. However, there are no practical animal models because P. falciparum infects human erythrocytes almost exclusively. Here we describe a reliable falciparum murine model of malaria by generating strains of P. falciparum in vivo that can infect immunodeficient mice engrafted with human erythrocytes. We infected NOD(scid/beta2m-/-) mice engrafted with human erythrocytes with P. falciparum obtained from in vitro cultures. After apparent clearance, we obtained isolates of P. falciparum able to grow in peripheral blood of engrafted NOD(scid/beta2m-/-) mice. Of the isolates obtained, we expanded in vivo and established the isolate Pf3D7(0087/N9) as a reference strain for model development. Pf3D7(0087/N9) caused productive persistent infections in 100% of engrafted mice infected intravenously. The infection caused a relative anemia due to selective elimination of human erythrocytes by a mechanism dependent on parasite density in peripheral blood. Using this model, we implemented and validated a reproducible assay of antimalarial activity useful for drug discovery. Thus, our results demonstrate that P. falciparum contains clones able to grow reproducibly in mice engrafted with human erythrocytes without the use of myeloablative methods.
Effect of Screening on Ovarian Cancer Mortality (2011)

Among women in the general US population, simultaneous screening with CA-125 and transvaginal ultrasound compared with usual care did not reduce ovarian cancer mortality. Diagnostic evaluation following a false-positive screening test result was associated with complications. Trial Registration clinicaltrials.gov Identifier: NCT00002540.
Platelet-activating factor triggers the phosphorylation and activation of MAP-2 kinase and S6 peptide kinase activity in human B cell lines. (1993)

Platelet activating factor (PAF), a phospholipid mediator produced by a variety of cell types, has potent biological activities in many cellular systems. We report that stimulation of B cell lines with PAF induced tyrosine phosphorylation of a protein, approximately 42 kDa in size. The PAF receptor antagonist WEB 2086 as well as the structural analogue PAF antagonist CV3988 inhibited the response, demonstrating that the tyrosine phosphorylation was attributable to PAF binding to a specific receptor. Immunoblot analysis, using an antibody to microtubule-associated protein-2 kinase, demonstrated the presence of a single protein species in unstimulated cells. PAF treatment induced the appearance of an additional species with a slightly reduced mobility in the gel. This PAF-inducible band had the same mobility on SDS-PAGE gels as the protein that was tyrosine-phosphorylated after PAF stimulation. Tyrosine phosphorylation of the 42-kDa protein was rapid, detectable within 30 s, and evident over a wide range of PAF concentrations (10(-7) to 10(-10) M). Treatment of cells with phorbol myristate acetate induced tyrosine phosphorylation of a protein with the same mobility in SDS-PAGE gels as the protein induced after PAF stimulation. Lysates from PAF-stimulated lymphoblastoid cells exhibited more than twice the ability to phosphorylate myelin basic protein than lysates from untreated cells. In addition, the ribosomal S6 peptide kinase activity (S6PK) was increased after PAF stimulation of the B cells. Taken together, the results suggest that PAF stimulation of B cells induces the tyrosine phosphorylation and activation of microtubule-associated protein-2 kinase.
RNA mutagenesis yields highly diverse mRNA libraries for in vitro protein evolution (2007)

The RNA based approach outlined here is rapid and simple to perform and generates large, highly diverse populations of proteins, each differing by only one or two amino acids from the parent protein. The practical implications of our results are that suitable improved protein candidates can be recovered from in vitro protein evolution approaches using significantly fewer rounds of mutagenesis and selection, and with little or no collateral damage to the protein or its mRNA.
Correction to Gd(III)-Labeled Peptide Nanofibers for Reporting on Biomaterial Localization in Vivo (2015)

ADVERTISEMENT RETURN TO ISSUEPREVAdditions and Correc...Additions and CorrectionsORIGINAL ARTICLEThis notice is a correctionCorrection to Gd(III)-Labeled Peptide Nanofibers for Reporting on Biomaterial Localization in VivoAdam T. Preslar, Giacomo Parigi, Mark T. McClendon, Samantha S. Sefick, Tyson J. Moyer, Chad R. Haney, Emily A. Waters, Keith W. MacRenaris, Claudio Luchinat, Samuel I. Stupp*, and Thomas J. Meade*Cite this: ACS Nano 2015, 9, 11, 11502Publication Date (Web):October 30, 2015Publication History Received15 October 2015Published online30 October 2015Published inissue 24 November 2015https://doi.org/10.1021/acsnano.5b06496Copyright © 2015 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views1372Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-AlertsSUBJECTS:Nanofibers,Peptides and proteins,Thermal annealing,Transmission electron microscopy Get e-Alerts
How Linker’s Modification Controls Swelling Properties of Highly Flexible Iron(III) Dicarboxylates MIL-88 (2011)

A series of organically modified iron(III) terephthalate MIL-88B and iron(III) 4,4'-biphenyl dicarboxylate MIL-88D flexible solids have been synthesized and characterized through a combination of X-ray diffraction, IR spectroscopy, and thermal analysis (MIL stands for Material from Institut Lavoisier). The swelling amplitude of the highly flexible MOFs tuned by introducing functional groups onto the phenyl rings shows a clear dependence on the steric hindrance and on the number of groups per aromatic ring. For instance, while the introduction of four methyl groups per spacer in dried MIL-88B results in a large permanent porosity, introducing two or four methyl groups in MIL-88D allows an easier pore opening in the presence of liquids without drastically decreasing the swelling magnitude. The influence of the degree of saturation of the metal center and the nature of the solvent on the swelling is also discussed. Finally, a computationally assisted structure determination has led to a proposal of plausible structures for the closed (dried) and open forms of modified MIL-88B and MIL-88D and to evaluation of their framework energies subject to the nature of the functional groups.
Prediction of total body water in infants and children (2005)

Secular trends in the nutritional status of infants and children are altering the relation between age or weight and TBW. Equations developed in previous decades significantly overestimate TBW in all age groups, especially infancy; however, the relation between TBW and weight may continue to change. This scenario is predicted to apply more generally to many aspects of paediatric clinical practice in which dosages are calculated on the basis of anthropometric data collected in previous decades.
Uniform Nickel Deposition into Ordered Alumina Pores by Pulsed Electrodeposition (2000)

Ordered arrays of nickel nanowires have been prepared using pulsed electrodeposition. Two self-patterning anodization processes were used to fabricate alumina pore matrices into which nickel was deposited from a Watts bath: two short millisecond deposition pulses followed by a long delay yielded almost 100 % filled pores (see Figure). Nanowire arrays are expected to have important applications as magnetic memories.
A Metalloproteinase Inhibitor Prevents Lethal Acute Graft-Versus-Host Disease in Mice (1997)

Tumor necrosis factor (TNF) and Fas ligand (FasL) have been implicated in the pathogenesis of graft-versus-host disease (GVHD), which is a major complication after allogeneic bone marrow transplantation. We examined here the ameliorating effect of a metalloproteinase inhibitor (KB-R7785) that inhibits TNF-alpha and FasL release in a lethal acute GVHD model in mice. Administration of KB-R7785 into (BALB/c x C57BL/6) F1 that received C57BL/6 spleen cells markedly reduced the mortality and weight loss in association with minimal signs of GVHD pathology in the liver, intestine, and hematopoietic tissues. The ameliorating effect of KB-R7785 was superior to that of anti-TNF-alpha antibody. Our results suggest that KB-R7785 could be a potent therapeutic agent for GVHD.
<title>POLARIS-II: an acousto-optic imaging spectropolarimeter for ground-based astronomy</title> (1992)

A compact, acousto-optic tunable filter (AOTF) imaging spectropolarimeter for ground based astronomy from 400-1100 nm has been constructed at NASA/GSFC. The key components of this instrument are a TeO<sub>2</sub> non-collinear AOTF, CCD camera, and an all-reflective optical relay assembly which uses a single elliptical mirror to produce side-by-side orthogonally polarized spectral images. The instrument was used at the Lowell Observatory 42-inch telescope for 'first light' planetary imaging and measurements of photometric standard stars. Narrow-band images of Saturn near 700 nm appear to show polarization effects which result from multiple scattering by aerosols. The instrument has recently been upgraded in order to integrate the RF drive electronics and eliminate contamination by scattered light. Design of the instrument and some initial results are presented.
Memory-Efficient Pipelined Architecture for Large-Scale String Matching (2009)

We propose a pipelined field-merge architecture for memory-efficient and high-throughput large-scale string matching (LSSM). Our proposed architecture partitions the (8-bit) character input into several bit-field inputs of smaller (usually 2-bit) widths. Each bit-field input is matched in a partial state machine (PSM) pipeline constructed from the respective bit-field patterns. The matching results from all the bit-fields in every pipeline stage are then merged with the help of an auxiliary table (ATB). This novel architecture essentially divides the LSSM problem with a continuous stream of input characters into two disjoint and simpler sub-problems: 1) O(character_bitwidth) number of pipeline traversals, and 2) O(pattern_length) number of table lookups. It is naturally suitable for implementation on FPGA or VLSI with on-chip memory. Compared to the bit-split approach, our field-merge implementation on FPGA requires 1/5 to 1/13 the total memory while achieving 25% to 54% higher clock frequencies.
Voxceleb: Large-scale speaker verification in the wild (2019)

The objective of this work is speaker recognition under noisy and unconstrained conditions. We make two key contributions. First, we introduce a very large-scale audio-visual dataset collected from open source media using a fully automated pipeline. Most existing datasets for speaker identification contain samples obtained under quite constrained conditions, and usually require manual annotations, hence are limited in size. We propose a pipeline based on computer vision techniques to create the dataset from open-source media. Our pipeline involves obtaining videos from YouTube; performing active speaker verification using a two-stream synchronization Convolutional Neural Network (CNN), and confirming the identity of the speaker using CNN based facial recognition. We use this pipeline to curate VoxCeleb which contains contains over a million ‘real-world’ utterances from over 6000 speakers. This is several times larger than any publicly available speaker recognition dataset. Second, we develop and compare different CNN architectures with various aggregation methods and training loss functions that can effectively recognise identities from voice under various conditions. The models trained on our dataset surpass the performance of previous works by a significant margin.
Strength loss after eccentric contractions is unaffected by creatine supplementation (2000)

This study's objective was to determine whether 14 days of dietary creatine supplementation preceding an injurious bout of eccentric contractions affect the in vivo strength loss of mouse anterior crural muscles. Three groups of nine mice each were fed a meal diet for 14 days, one group at each of three levels of creatine supplementation (i.e., 0, 0.5, and 1% creatine). Electrically stimulated concentric, isometric, and eccentric contraction torques produced about the ankle were measured both before and after a bout of 150 eccentric contractions. Tibialis anterior muscle creatine concentration was significantly increased by the supplementation, being 12% higher in the mice fed the 1% creatine diet compared with control mice. After the bout of eccentric contractions, the reductions in torque (i.e., 46-58%) were similar for the isometric contraction, all eccentric contractions, and the slow (i.e., </=200 (o)/s) concentric contractions; above 200 (o)/s, the percent reduction in concentric torque increased progressively to 85-88% at 1,000-1,200 (o)/s. However, there was no effect of creatine supplementation on the isometric torque loss or on the torque loss at any eccentric or concentric angular velocity (P >/= 0.62). In conclusion, a moderate increase in muscle creatine concentration induced by dietary supplementation in mice does not affect the strength loss after eccentric contractions.
Tumor cells escape suicide gene therapy by genetic and epigenetic instability (2004)

Transfer and expression of suicide genes is one cornerstone of cancer gene therapy and is also considered as a proactive tool to enhance the safety of somatic transgenesis. Here we addressed whether retrovirus-mediated suicide gene therapy would result in a predictable antitumor efficiency, given that problems related to gene transfer are solved or that the suicide gene is used in a proactive approach. Using retroviral vectors encoding the thymidine kinase gene of herpes simplex virus, we transduced EL-4 lymphoma cells and induced experimental tumors in congeneic C57Bl/6 mice. Systemic administration of ganciclovir (GCV) resulted in remission of transduced clonal and polyclonal tumors in vivo. However, GCV-resistant relapses occurred and were found to be associated with postinsertional alterations of transgene structure or loss of the entire transgene. Complete loss of a retrovirally marked fusion chromosome was confirmed by spectral karyotyping. Transgene silencing occurred in another clone. We conclude that genetic as well as epigenetic instability related to biologic features of the tumor, the insertion site, and the vector represent relevant limitations of retroviral suicide gene therapy. Considering the mechanisms of escape identified here, the proactive use of suicide genes to prevent complications of insertional mutagenesis may still be efficient.
[A retrospective study on the development of inhibitors in Japanese hemophiliacs (second report, 1994 study). Research Group of Blood Products for Hemophilia Inhibitor]. (1998)

In this report, we discuss the findings of a 1994 retrospective study concerning the rate of inhibitor formation in Japanese hemophiliacs. The study was the second of its kind, following on the first in 1991 (Kamiya et al. 1995 Int. J. Hematology). The records of 77 medical institutions were examined. Inhibitors were found in 6.50% (140 of 2154) of the patients with hemophilia A (HA), and 5.21% (22 of 422) of those with hemophilia B (HB). The median age for antibody formation was 10.7 years in patients with HA, and 4.5 years in those with HB. The median period (exposure period) from initial plasma factor concentrates exposure to inhibitor formation was 46 days and 20 days, respectively, in the HA and HB patients. Among the HA inhibitor patients, those with a large deletion or a nonsense mutation were aged 17.4 years or less (0.58, 1.7, 3.5, 5.5, 7.0 and 17.4), whereas those with intron 22 inversion were aged 55.7 years or less (1.3, 1.3, 1.8, 33.0, 36.1, 37.7, 43.9, 47.9 and 55.7).