gebhart/patrepeval huggingface dataset:
SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains (2012)

The synthetic biology toolkit contains a growing number of parts for regulating transcription and translation, but very few that can be used to control protein association. Here we report characterization of 22 previously published heterospecific synthetic coiled-coil peptides called SYNZIPs. We present biophysical analysis of the oligomerization states, helix orientations, and affinities of 27 SYNZIP pairs. SYNZIP pairs were also tested for interaction in two cell-based assays. In a yeast two-hybrid screen, >85% of 253 comparable interactions were consistent with prior in vitro measurements made using coiled-coil microarrays. In a yeast-signaling assay controlled by coiled-coil mediated scaffolding, 12 SYNZIP pairs were successfully used to down-regulate the expression of a reporter gene following treatment with α-factor. Characterization of these interaction modules dramatically increases the number of available protein interaction parts for synthetic biology and should facilitate a wide range of molecular engineering applications. Summary characteristics of 27 SYNZIP peptide pairs are reported in specification sheets available in the Supporting Information and at the SYNZIP Web site [http://keatingweb.mit.edu/SYNZIP/].
Synthesis and release of B-lymphocyte stimulator from myeloid cells (2001)

B-lymphocyte stimulator (BLyS) is a recently identified novel member of the tumor necrosis factor ligand superfamily shown to exist in a membrane-bound and soluble form. BLyS was found to be specifically expressed on cells of myeloid lineage and to selectively stimulate B-lymphocyte proliferation and immunoglobulin production. The expression of a cytokine involved in potentiation of humoral immune responses, such as BLyS, is expected to be strictly controlled. The goal of the present study was to examine regulation of BLyS levels in monocytic cells in response to cytokines and during their differentiation to macrophages and dendritic cells. The presence of BLyS on the cell surface and in the culture medium of both normal blood monocytes and on tumor cells of myelomonocytic origin was demonstrated. BLyS gene expression and levels of membrane-associated and soluble BLyS were found to be regulated by cytokines, in particular interferon (IFN)-gamma and to a lesser extent interleukin-10 (IL-10). The expression of BLyS on monocyte membranes was retained following differentiation into macrophages, but detection on the surface of monocyte-derived dendritic cells required stimulation with IFN-gamma. Both IFN-gamma and IL-10 enhanced the release of soluble BLyS that was active in B-cell proliferation assays. Cells transfected with BLyS complementary DNA mutated in a predicted cleavage site failed to release BLyS into the culture medium, thereby suggesting that soluble BLyS was derived from the membrane form. These results provide further support for an important role for BLyS expressed in myeloid cells in B-cell expansion and antibody responses.
Characteristics and partial purification of a novel cytosolic, magnesium-independent, neutral sphingomyelinase activated in the early signal transduction of 1 alpha,25-dihydroxyvitamin D3-induced HL-60 cell differentiation. (1994)

Treatment of HL-60 cells with a 1 alpha,25-dihydroxyvitamin D3 induces activation of a neutral sphingomyelinase (SMase), resulting in a decrease in sphingomyelin (SM) levels and an increase in ceramide levels in a proposed "sphingomyelin cycle" of cell regulation (Okazaki, T., Bell, R., and Hannun, Y. (1989) J. Biol. Chem. 264, 19076-19080). Cell-permeable synthetic ceramides induce HL-60 cell differentiation toward a monocytic lineage without conversion to sphingosine, suggesting that ceramide is a lipid mediator of cell differentiation (Okazaki, T., Bielawska, A., Bell, R., and Hannun, Y. (1990) J. Biol. Chem. 265, 15823-15831). In this study, we investigated a novel SMase that was activated 2-2.5 h after treatment of cells with 1 alpha,25-dihydroxyvitamin D3. The activated SMase was localized to the cytosolic fraction. It was inhibited by copper, ferric iron, and zinc and showed optimal activity at pH 7.5. A mixed micellar assay was developed for the enzyme, with optimal activity achieved at 12 mol% SM in Triton X-100 mixed micelles and at 20 mol% SM in deoxycholate micelles. The activity was modestly enhanced by phosphatidic acid, phosphatidylserine, or phosphatidylinositol, but not by other major phospholipids. Purification was performed by chromatography on DEAE anion-exchange, Q-Sepharose Fast Flow, hydroxylapatite, sphingosylphosphocholine affinity, and Superose 12 gel filtration columns. Two peaks of activity with molecular masses of 45 and 95 kDa were resolved by gel filtration chromatography on Superose 12. The specific activities of the purified 45- and 95-kDa enzymes were 2780 and 2790 nmol/mg/h, respectively. These data identify a novel cytosolic, magnesium-independent, neutral SMase(s) that is activated during cell differentiation.
dpy-18 Encodes an α-Subunit of Prolyl-4-Hydroxylase in Caenorhabditis elegans (2000)

Collagen is an extracellular matrix (ECM) component encoded by a large multigene family in multicellular animals. Procollagen is post-translationally modified by prolyl-4-hydroxylase (EC 1.14.11.2) before secretion and participation in ECM formation. Therefore, collagen processing and regulation can be studied by examining this required interaction of prolyl-4-hydroxylase with procollagen. High-resolution polymorphism mapping was used to place the Caenorhabditis elegans dpy-18 gene on the physical map, and we show that it encodes a prolyl-4-hydroxylase alpha catalytic subunit. The Dpy phenotype of dpy-18(e364) amber mutants is more severe when this mutation is in trans to the noncomplementing deficiency tDf7, while the dpy-18(e499) deletion mutant exhibits the same phenotype as dpy-18(e499)/tDf7. Furthermore, dpy-18 RNA interference (RNAi) in wild-type worms results in Dpy progeny, while dpy-18 (RNAi) in dpy-18(e499) mutants does not alter the Dpy phenotype of their progeny. These observations suggest that the dpy-18 null phenotype is Dpy. A dpy-18::gfp promoter fusion construct is expressed throughout the hypodermis within the cells that abundantly produce the cuticle collagens, as well as in certain head and posterior neurons. While prolyl-4-hydroxylase has been studied extensively by biochemical techniques, this is the first report of a mutationally defined prolyl-4-hydroxylase in any animal.
Amyloid A proteins in different species. (1984)

Amyloid A (AA) proteins were purified from canine and bovine idiopathic amyloid and casein-induced hamster amyloid. The molecular weights of these proteins were in the same range (8,000-10,000) as those reported for AA proteins from man and other animal species. The amino acid compositions were comparable as well. Antisera against human, bovine, canine and hamster protein AA were tested with the indirect immunoperoxidase antiperoxidase (PAP) method and the indirect immunofluorescence technique on amyloid-containing human, bovine, canine and hamster tissue sections. Antihuman protein AA serum did not cross-react with hamster protein AA. In all the other combinations, cross-reactions were observed. Because of the high similarity of these different AA proteins, the amyloid diseases in the species mentioned can serve as interchangeable models to investigate the pathogenesis of AA amyloidosis.
Fetal tissue transplantation: can it be morally insulated from abortion? (1991)

Ethical controversy over transplantation of human fetal tissue has arisen because the source of tissue is induced abortions. Opposition to such transplants has been based on various arguments, including the following: rightful informed consent cannot be obtained for use of fetal tissue from induced abortions, and fetal tissue transplantation might result in an increase in the number of abortions. These arguments were not accepted by the National Institutes of Health (NIH) Human Fetal Tissue Transplantation Research Panel. The majority opinion of the panel stated that abortion and fetal tissue use are entirely separate issues, and that tissue use is ethically acceptable because it can be morally insulated from the issue of abortion. In support of this view, panel members and others have replied to the arguments put forward by opponents of fetal tissue use. However, replies to the two arguments mentioned above have been unsatisfactory, and the shortcomings of those replies are identified herein. Examination of the arguments pro and con suggests that fetal tissue use cannot be completely insulated from the issue of abortion. Thus, in seeking an ethical justification for fetal tissue transplantation we must consider reasons other than those put forward by the NIH panel. In this paper it is argued that whatever wrong is involved in using fetal tissue from induced abortions must be balanced against the benefits for patients, and it is on this basis that fetal tissue transplantation can be ethically justified.
Macrolide antibiotics M-4365 produced by Micromonospora. II. Chemical structures. (1977)

By physiochemical analyses and chemical procedures, the structures of a series of basic 16-membered macrolide antibiotics, M-4365, A1, A2, A3, G1, G2 and G3 were elucidated, and it was found that M-4365 A1, G1 and G2 were novel, while M-4365 A2, A3 and G3 were identical with rosamicin, juvenimicins A4 and B1, respectively.
Old fashioned sodium bicarbonate baths for the treatment of psoriasis in the era of futuristic biologics: An old ally to be rescued (2005)

We maintain that even with sophisticated immunosuppressive and highly specific anti-cytokine and anti-chemokines treatments available for the treatment of psoriasis, an older and often forgotten treatment can still play a role.
脱水旋流器帮了厄尔戈(Ergo)的忙 (1999)

厄尔戈砂矿磨矿车间位于南非东兰德(East Rand)的布拉潘(Brakpan),于1990年7月投产,系为3对布拉潘矿砂堆的老金尾矿进行磨矿。磨矿产品及其它矿泥泵送至位于斯普林斯(Springs)的中央厄尔戈金回收厂进行金萃取。磨矿产品的脱水对于降低泵送成本及管道运送速度十分重要。据该公司报道,选择了Multotec脱水旋流器而不是常规的高效浓密机节约了大笔的基建与生产成本。该磨矿车间有两条平行作业线,各由1台与三组Multotec旋流器成闭路作业的4.27m(直径)×10m(长度)处理能力为160-180t/h的Osborn半自磨机组成。给矿由储矿槽经一次磨机泵池给到一台1200mm直径的橡胶衬里的旋流器。这种南非独有的旋流器可能是世界上最大的旋流器。一次分级旋流器底流进入半自磨磨机,其磨矿产品自重流入一次磨机泵池,旋流器溢流则进入一台900mm的橡胶衬里的二次
P3–126: Vitamin B12, holotranscobalamin and risk of cognitive decline: 10–year follow–up of the Oxford Healthy Aging Project (2006)

Elevated total homocysteine (tHcy) levels are associated with cognitive impairment and dementia, but it is uncertain if these associations are causal. Previous studies have shown that elevated tHcy in unimpaired older people predicts risk of dementia, but the relevance of low folate or vitamin B12 (B12) status for cognitive decline is uncertain. To examine the associations of B12 and folate status with cognitive decline between year 0 and 2, and year 0 and 10, respectively, in the Oxford Healthy Aging Project (n=2741). Cognitive function was assessed sequentially over 10 years using the Mini–Mental State Examination (MMSE). Blood was collected from 1648 individuals at year 2 and from 472 individuals at year 10. Serum levels of B12, holotranscobalamin (holoTC), folate, tHcy and methylmalonic acid (MMA) were measured. Repeated measurements over time on individuals were analyzed using random effects mixed models to examine associations of cognitive decline with vitamin status after adjustment for age, sex, smoking, education, apoE and prior vascular disease. Individuals were also classified as having significant cognitive decline at year 10 if their MMSE score declined by ≥3 points/10 years. The self–correlation (r) for MMSE scores at 6 months (n=352) was high (r=0.82). The self–correlation for biochemical markers of B12 status at 8 years between measurements was also high (r=0.68 for holoTC, 0.59 for B12, 0.53 for tHcy and 0.70 for MMA), but not for folate (r=0.23). After adjustment for relevant covariates, low holoTC was associated with more rapid cognitive decline at year 2 (p=0.03), but this association was no longer significant at year 10. Individuals with significant cognitive decline at year 10 (n=154) compared with those without it (n=520) had lower serum levels at year 2 of B12 (p<0.001), higher tHcy (p=0.04) and MMA levels (p=0.001), respectively, but there were no differences in folate levels. The rates of cognitive decline were more rapid in individuals with low holoTC levels at baseline. Low B12 status at baseline also predicted cognitive decline at 10 years. Large trials are required to assess the clinical relevance of B12 supplementation for maintenance of cognitive function in older people.
The effect of valproate on blood metabolite concentrations in spontaneously diabetic, ketoacidotic, BB/E Wistar rats. (1985)

Valproate is an effective anticonvulsant which is rarely associated with hepatotoxicity. It has profound effects on the intermediary metabolism of isolated rat hepatocytes. In this paper the effect of valproate on blood metabolite concentrations in spontaneously diabetic BB/E rats has been studied. Valproate causes a marked fall in blood ketone body concentrations and a smaller fall in blood glucose concentrations following either oral or intraperitoneal administration. Valproate may have a role in the treatment of patients with "brittle" diabetes.
Motion and structure from line correspondences; closed-form solution, uniqueness, and optimization (1992)

This work discusses estimating motion and structure parameters from line correspondences of a rigid scene. The authors present a closed-form solution to motion and structure parameters from line correspondences through three monocular perspective views. The algorithm makes use of redundancy in the data to improve the accuracy of the solutions. The uniqueness of the solution is established, and necessary and sufficient conditions for degenerate spatial line configurations are given. Optimization has been employed to further improve the accuracy of the estimates in the presence of noise. Simulations have shown that the errors of the optimized estimates are close to the theoretical lower error bound.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Risk factors and clinical manifestations of pre‐eclampsia (2000)

Nulliparity and hypertension increased the risk for each subgroup of pre-eclampsia, but high maternal weight, previous pre-eclampsia and smoking were not consistently associated with each clinical subtype. This observation may suggest that heterogeneous clinical manifestations of pre-eclampsia may be preceded by different pathological mechanisms.
Antibody fucosylation differentially impacts cytotoxicity mediated by NK and PMN effector cells (2008)

Glycosylation of the antibody Fc fragment is essential for Fc receptor-mediated activity. Carbohydrate heterogeneity is known to modulate the activity of effector cells in the blood, in which fucosylation particularly affects NK cell-mediated killing. Here, we investigated how the glycosylation profile of 2F8, a human IgG(1) monoclonal antibody against epidermal growth factor receptor in clinical development, impacted effector function. Various 2F8 batches differing in fucosylation, galactosylation, and sialylation of the complex-type oligosaccharides in the Fc fragment were investigated. Our results confirmed that low fucose levels enhance mononuclear cell-mediated antibody-mediated cellular cytotoxicity (ADCC). In contrast, polymorphonuclear cells were found to preferentially kill via high-fucosylated antibody. Whole blood ADCC assays, containing both types of effector cells, revealed little differences in tumor cell killing between both batches. Significantly, however, high-fucose antibody induced superior ADCC in blood from granulocyte colony-stimulating factor-primed donors containing higher numbers of activated polymorphonuclear cells. In conclusion, our data demonstrated for the first time that lack of fucose does not generally increase the ADCC activity of therapeutic antibodies and that the impact of Fc glycosylation on ADCC is critically dependent on the recruited effector cell type.
Eslicarbazepine Acetate: A Double‐blind, Add‐on, Placebo‐controlled Exploratory Trial in Adult Patients with Partial‐onset Seizures (2007)

Eslicarbazepine acetate was efficacious and well tolerated as an adjunctive therapy of refractory epileptic patients.
Enteropathogenicity of various isolates of Treponema hyodysenteriae (1977)

Isolates of Treponema hyodysenteriae from 25 geographically separated outbreaks of swine dysentery were tested for their ability to produce the disease. Clinical signs and lesions typical of acute swine dysentery were produced in 52 of 68 (75%) susceptible specific pathogen-free pigs that had been orally inoculated with pure cultures of 23 of 25 beta-hemolytic isolates. In addition, 13 weakly beta-hemolytic isolates of nondysentery origin with morphology similar to T. hyodysenteriae did not produce disease when orally inoculated into susceptible specific pathogen-free pigs. Two of these latter isolates, Puppy and B296, and one pathogenic, beta-hemolytic isolate failed to produce disease when orally inoculated into puppies.
Insulin-releasing pituitary cells as a model for somatic cell gene therapy in diabetes mellitus (1994)

Insulin delivery by somatic cell gene therapy was evaluated using murine pituitary AtT20MtIns-1.4 cells. These cells have been stably transfected to release human insulin by the introduction of a recombinant plasmid bearing a human preproinsulin cDNA under the control of a zinc-sensitive metallothionein promoter. 6 x 10(7) AtT20MtIns-1.4 cells were implanted subcutaneously into streptozotocin-diabetic mice immunosuppressed with cyclosporin A. Release of human insulin was assessed using a specific plasma human C-peptide assay. On days 1 and 2 after implantation human C-peptide concentrations were about 0.02 pmol/ml. Consumption of zinc sulphate solution (500 mg/l) as drinking fluid for days 3-5 increased plasma human C-peptide concentrations to 0.11 +/- 0.01 pmol/ml (mean +/- S.E.M.), n = 11, P < 0.01, and concentrations declined when zinc was discontinued. The extent of hyperglycaemia was slightly lower (P < 0.05) than in a group implanted with non-transfected AtT20 cells. The study was terminated after 9 days, and tumour-like aggregations of implanted cells were identified at autopsy. These comprised a large necrotic core with insulin-containing cells at the periphery. The study provides support for the view that somatic cell gene therapy offers a potential approach to insulin delivery in diabetes mellitus.
Three-dimensional imaging system (1994)

A vision system that acquires spatial coordinates (range data) of three-dimensional objects by triangulation using structured light is presented. The developed system requires only a single image and guarantees unambiguous correspondence by using a uniquely encoded light pattern. Using a single image enables the system to perform in a dynamic environment, which contains moving objects. An example is presented.
Drug Facts and Comparisons 2013 (1999)

Facts and contains up-to-date, comprehensive information on over 22,000 Rx and 6,000 OTC items grouped by therapeutic category for ease of comparison. The book answers questions about actions, indications and contraindications; warnings and precautions; interactions between drugs; adverse reactions; administration, dosage, and overdosage; patient information. Comparisons provided include drug to drug; different dosage forms; name brands are compared to name brands and to generics. Facts and Comparisons are also given on Centers for Disease Control biologicals and antiparasitic drugs; IV solutions; therapeutic and diagnostic ophthalmic preparations; diagnostic aids and radiographic contrast media and products; NCI investigational chemotherapeutic drugs; combination cancer chemotherapy regimens. This hardbound edition includes the Drug Identifier 2008 CD-ROM, the premier tool for correct drug identification. It ensures accuracy by searching through more than 5,000 color images in seconds. It enables to search by drug name, imprint, NDC code, color, shape, manufacturer, coating, scoring, clarity, and flavor.
Efficacy and Safety of Single and Repeated Administration of 1 Gram Intravenous Acetaminophen Injection (Paracetamol) for Pain Management after Major Orthopedic Surgery (2005)

Intravenous acetaminophen, 1 g, administered over a 24-h period in patients with moderate to severe pain after orthopedic surgery provided rapid and effective analgesia and was well tolerated.
Direct Monitoring of Energy Lost Due to Soiling on First Solar Modules in California (2012)

The optical loss caused by the accumulation of dirt and other contaminants on solar collectors is the third most important meteorological input, after insolation and air temperature, that determines energy yield in a photovoltaic power plant. First Solar is monitoring the impact of dust accumulation on solar panels using a methodology similar to that proposed by Ryan and colleagues in a long-term study in Eugene, OR. The method seeks to be a practical and automated technique for measuring the soiling rate, foregoing complex equipment such as <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I</i> ( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">V</i> ) curve tracers, and eliminating other performance-influencing factors. The test setup includes several First Solar plane-of-array modules that are allowed to accumulate natural contaminants, and several controls held clean by regular washing. This study reveals clear region-specific soiling trends as well as insight into the amount of rainfall required for full recovery of module performance. Soiling rates of up to 11.5% per month are observed in heavy agricultural areas. As little as 0.5 mm of rainfall is sufficient to completely clean a dirty frameless module in regions with lighter soiling rates.
Inhibitor development in haemophilia A: the role of von Willebrand factor/factor VIII concentrates (2007)

The presence of inhibitors that neutralize the function of factor VIII (FVIII) decreases the haemostatic efficacy of replacement clotting factor concentrate and increases morbidity among patients with haemophilia A. Certain genetic and environmental variables have been linked to a higher incidence of inhibitors. Conversely, the presence of von Willebrand factor (VWF) in some plasma-derived FVIII products may provide some measure of protection against inhibitor development, although the evidence is not conclusive. Clinical trials are needed to resolve this issue and determine the appropriate role of VWF-containing FVIII concentrates in the treatment of haemophilia A patients.
Progress in understanding the pathogenesis of the anemia of chronic disease [see comments] (1992)

Abstract Improved understanding of the inflammatory response and the identification and characterization of the specific cytokines involved, as well as improved understanding of erythropoiesis, and the availability of recombinant human growth factors such as EPO, have greatly enhanced our appreciation of the pathogenesis of ACD by allowing development of a number of informative models for studying this syndrome. It appears that a variety of cytokines are involved in all aspects of the pathogenesis of ACD, from the inhibition of erythroid progenitors and EPO production to impairment of iron release. A schematic of the contributions of some of these cytokines to the development of ACD is shown in Fig 6. The exact biochemical mechanisms by which these effects occur is still to be determined. The progress outlined in this report has allowed us to develop a more precise understanding of the pathogenesis of this common and important clinical syndrome. In 1983, Hansen subtitled a review of ACD “A Bag of Unsolved Questions.” Although this description is still accurate, our understanding of ACD has now developed to the point where we can offer a more defined subtitle: “A Bag of Cytokines.”