gebhart/patrepeval huggingface dataset:
Induction of immunity to prostate cancer antigens: results of a clinical trial of vaccination with irradiated autologous prostate tumor cells engineered to secrete granulocyte-macrophage colony-stimulating factor using ex vivo gene transfer. (1999)

Vaccination with irradiated granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting gene-transduced cancer vaccines induces tumoricidal immune responses. In a Phase I human gene therapy trial, eight immunocompetent prostate cancer (PCA) patients were treated with autologous, GM-CSF-secreting, irradiated tumor vaccines prepared from ex vivo retroviral transduction of surgically harvested cells. Expansion of primary cultures of autologous vaccine cells was successful to meet trial specifications in 8 of 11 cases (73%); the yields of the primary culture cell limited the number of courses of vaccination. Side effects were pruritus, erythema, and swelling at vaccination sites. Vaccine site biopsies manifested infiltrates of dendritic cells and macrophages among prostate tumor vaccine cells. Vaccination activated new T-cell and B-cell immune responses against PCA antigens. T-cell responses, evaluated by assessing delayed-type hypersensitivity (DTH) reactions against untransduced autologous tumor cells, were evident in two of eight patients before vaccination and in seven of eight patients after treatment. Reactive DTH site biopsies manifested infiltrates of effector cells consisting of CD45RO+ T-cells, and degranulating eosinophils consistent with activation of both Th1 and Th2 T-cell responses. A distinctive eosinophilic vasculitis was evident near autologous tumor cells at vaccine sites, and at DTH sites. B-cell responses were also induced. Sera from three of eight vaccinated men contained new antibodies recognizing polypeptides of 26, 31, and 150 kDa in protein extracts from prostate cells. The 150-kDa polypeptide was expressed by LNCaP and PC-3 PCA cells, as well as by normal prostate epithelial cells, but not by prostate stromal cells. No antibodies against prostate-specific antigen were detected. These data suggest that both T-cell and B-cell immune responses to human PCA can be generated by treatment with irradiated, GM-CSF gene-transduced PCA vaccines.
Neurotrophic effect of hepatocyte growth factor on central nervous system neurons in vitro (1996)

Although the expression of hepatocyte growth factor (HGF) and its receptor, proto-oncogene c-met, has been demonstrated in the central nervous system (CNS), the function of HGF in the CNS was not fully understood. In the present studies, we determined the effects of HGF on neuronal development in neocortical explant and mesencephalic neurons obtained from embryonic rat brain. HGF clearly enhanced neurite outgrowth in neocortical explants. In the mesencephalic culture, the number of tyrosine hydroxylase (TH)-positive neurons was significantly higher in the HGF-treated wells and the neurites of the TH-positive neurons appear to be more developed. Moreover, the dopamine uptake into mesencephalic neurons was also enhanced by HGF treatment, indicating that HGF promotes the survival and/or maturation of mesencephalic dopaminergic neurons. In both neocortical explants and mesencephalic neurons, c-met autophosphorylation was induced by HGF and MAP kinase activation was also detected in the neocortical explant. Furthermore, Western blot analysis of the cultured CNS cells revealed that HGF was expressed mainly in microglia. These results suggest that HGF from microglia has neurotrophic activity on the CNS neurons and plays significant roles in the development of the CNS.
Osmoconditioning of Beet Seeds to Improve Emergence and Yield in Cold Soil1 (1983)

Abstract Table beet ( Beta vulgaris L.) seeds are characteristically slow germinating, and have a large time spread of germination. These characteristics interfere with the early establishment of a uniform, vigorous stand of seedlings in cold wet soil. This study was undertaken to determine if osmoconditioning (OC) of table beet seeds in polyethylene glycol 6000 (PEG) and MgSO 4 solutions and/or other measures would improve germination and/or reduce germination time and thereby improve emergence, seedling establishment and yield. Seed balls of ‘Ruby Queen’ and ‘Cardenal’ were osmoconditioned in −1.2 and −1.18 MPa solutions of PEG and MgSO 4 , respectively. Treatments were performed at 15 °C for 7 days in the light. The benefits of OC were not lost by drying back the seeds. Aeration during OC followed by a 2 min rinse with water was the most effective treatment in improving germination or emergence in laboratory and growth chamber studies. In the 1980 field trial, OC in PEG improved the rate of emergence by 2 to 3 days, the stand by 40% and the root, top, and total yield by 40, 66, and 59%, respectively. In 1982, seedling emergence was biphasic. The first phase lasted 23 days and showed large differences in rate of emergence and stand between osmotically treated and untreated seeds and resembled the 1980 emergence pattern. The second phase commenced after a 30 mm rain with a resurgence of emergence in all treatments. The resurgence rate was highest in untreated seeds and this reduced the treatment differences in stand and yield. The resurgence was attributed to invigoration of the non‐germinated seed by low soil water potential (drought). Several MgSO 4 ‐OC treatments increased the number of roots but decreased root size.
Design of retrovirus vectors for transfer and expression of the human beta-globin gene (1988)

Regulated expression of the human beta-globin gene has been demonstrated in cultured murine erythroleukemia cells and in mice after retrovirus-mediated gene transfer. However, the low titer of recombinant viruses described to date results in relatively inefficient gene transfer, which limits their usefulness for animal studies and for potential gene therapy in humans for diseases involving defective beta-globin genes. We found regions that interfered with virus production within intron 2 of the beta-globin gene and on both sides of the gene. The flanking regions could be removed, but intron 2 was required for beta-globin expression. Inclusion of beta-globin introns necessitates an antisense orientation of the gene within the retrovirus vector. However, we found no effect of the antisense beta-globin transcription on virus production. A region downstream of the beta-globin gene that stimulates expression of the gene in transgenic mice was included in the viruses without detrimental effects on virus titer. Virus titers of over 10(6) CFU/ml were obtained with the final vector design, which retained the ability to direct regulated expression of human beta-globin in murine erythroleukemia cells. The vector also allowed transfer and expression of the human beta-globin gene in hematopoietic cells (CFU-S cells) in mice.
Carbon Fibers: Precursors, Manufacturing, and Properties (2012)

Abstract The properties, structure, and processing of carbon fibers are reviewed. Carbon fibers are made from several precursors, with PAN being the dominating precursor in the market. Carbon fibers have high tensile strength, high modulus (up to the theoretical limit of around 1000 GPa), and low density, depending on the structure and processing in very limited combinations. Both the structure and composition of the precursor affect the properties of the resulting carbon fibers significantly. Although the essential processes for carbon fiber production are similar, different precursors require different processing conditions in order to achieve improved performance. Future developments are discussed. magnified image
The Importance of Drug Metabolism Studies for Efficient Drug Discovery and Development. (1996)

Drug action is the result of interaction with target sites, for both desired (pharmacological) and undesired (toxic) actions, modulated by the transfer processes, the pharmacokinetic variables of absorption, distribution, metabolism and elimination, by which the drug enters and leaves the body. There exist in general better relationships between effect and internal exposure, i.e. target concentrations, most frequently related to plasma concentration, than with the external dose offered. Drug metabolism and pharmacokinetic studies have essential roles to play in all stages of the research and development process, ideally being involved from the pre-nomination phase in drug discovery through to post-marketing surveillance. There occurs far more inter and intraspecies variation, in animals and humans, in the factors influencing the nature and extent of internal exposure, than in the sensitivity of drug targets and this pharmacokinetic variability is the cause of major problems in drug development. The origins of this may be termed “pharmacokinetic defects” and include, inter alia, poor absorption, very short or very long half-life, enzyme induction and high first pass effect. Failure to take these into consideration can cause expensive delay and/or failure during development and make an approved drug vulnerable in the marketplace. It will be argued that the thoughtful inclusion of new feedback loops will improve decision making at various stages during drug development. These should be based on quality metabolic and pharmacokinetic data and exploit the opportunities which the new biology offers for predicting metabolic pathways, anticipating kinetic variability and understanding mechanisms of toxicity. Such improved decision making should contribute to enhanced time- and cost-efficiency of development and ultimately lead to safer, more easily used, drugs.
A novel mechanism of retrovirus inactivation in human serum mediated by anti-alpha-galactosyl natural antibody. (1995)

Type C retroviruses endogenous to various nonprimate species can infect human cells in vitro, yet the transmission of these viruses to humans is restricted. This has been attributed to direct binding of the complement component C1q to the viral envelope protein p15E, which leads to classical pathway-mediated virolysis in human serum. Here we report a novel mechanism of complement-mediated type C retrovirus inactivation that is initiated by the binding of "natural antibody" [Ab] (anti-alpha-galactosyl Ab) to the carbohydrate epitope Gal alpha 1-3Gal beta 1-4GlcNAc-R expressed on the retroviral envelope. Complement-mediated inactivation of amphotropic retroviral particles was found to be restricted to human and other Old World primate sera, which parallels the presence of anti-alpha-galactosyl natural Ab. Blockade or depletion of anti-alpha-galactosyl Ab in human serum prevented inactivation of both amphotropic and ecotropic murine retroviruses. Similarly, retrovirus was not killed by New World primate serum except in the presence of exogenous anti-alpha-galactosyl Ab. Enzyme-linked immunosorbent assays revealed that the alpha-galactosyl epitope was expressed on the surface of amphotropic and ecotropic retroviruses, and Western blot analysis further localized this epitope to the retroviral envelope glycoprotein gp70. Finally, down-regulation of this epitope on the surface of murine retroviral particle producer cells rendered them, as well as the particles liberated from these cells, resistant to inactivation by human serum complement. Our data suggest that anti-alpha-galactosyl Ab may provide a barrier for the horizontal transmission of retrovirus from species that express the alpha-galactosyl epitope to humans and to other Old World primates. Further, these data provide a mechanism for the generation of complement-resistant retroviral vectors for in vivo gene therapy applications where exposure to human complement is unavoidable.
Temporal association of cellular immune responses with the initial control of viremia in primary human immunodeficiency virus type 1 syndrome (1994)

Virologic and immunologic studies were performed on five patients presenting with primary human immunodeficiency virus type 1 (HIV-1) infection. CD8+ cytotoxic T lymphocyte (CTL) precursors specific for cells expressing antigens of HIV-1 Gag, Pol, and Env were detected at or within 3 weeks of presentation in four of the five patients and were detected in all five patients by 3 to 6 months after presentation. The one patient with an absent initial CTL response had prolonged symptoms, persistent viremia, and low CD4+ T-cell count. Neutralizing antibody activity was absent at the time of presentation in all five patients. These findings suggest that cellular immunity is involved in the initial control of virus replication in primary HIV-1 infection and indicate a role for CTL in protective immunity to HIV-1 in vivo.
Cytoplasmic and periplasmic expression of a highly basic protein, human interleukin 4, inEscherichia coli (1990)

Human IL-4 (hIL-4) has been cloned from a human T cell line based on its homology to the murine IL-4 cDNA sequence. We have compared cytoplasmic and extra-cytoplasmic expression of this basic protein in Escherichia coli using various combinations of promoters, replicons and host strains. Strains producing a cytoplasmic product were most successful at heterologous protein expression, producing up to 500 mg/l of an inactive aggregated form of the protein. The biological activity of the protein could be restored by refolding the protein with guanidine hydrochloride and glutathione giving a specific activity identical to that of IL-4 derived from CHO cell lines stably transformed with an hIL-4 expression plasmid. Strains designed to secrete human IL-4 into the periplasmic space produced far less protein (approximately 5 mg/l). However, a significant fraction of this protein was detected in the culture medium. This fraction appeared to be soluble after ultracentrifugation, and demonstrated high specific activity without refolding. Leakage of heterologous protein into the culture medium may be a viable way to recover biologically active products without relying on the denaturation and refolding in vitro that can, at times, yield incorrectly folded gene product.
Elementary Linear Algebra with Applications (1991)

1 - Linear Equations And Matrices 2 - Solving Linear Systems 3 - Determinants 4 - Real Vector Spaces 5 - Inner Product Spaces 6 - Linear Transformations and Matrices 7 - Eigenvalues and Eigenvectors 8 - Applications of Eigenvalues and Eigenvectors (Optional) 9 - MATLAB for Linear Algebra 10 - MATLAB Exercises A P P E N D I X A Preliminaries A P P E N D I X B Complex Numbers A P P E N D I X C Introduction to Proofs
Sugar processing and by-products of the sugar industry (2001)

Sugar processing and by-products of the sugar industry , Sugar processing and by-products of the sugar industry , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Complementary addressed modification and cleavage of a single stranded DNA fragment with alkylating oligonucleotide derivatives (1986)

A single stranded DNA fragment was modified with alkylating derivatives of oligonucleotides complementary to a certain nucleotide sequences in the fragment. The derivatives carried aromatic 2-chloroethylamino groups at their 3'- or 5'-terminal nucleotide residues. Some of the derivatives carried both alkylating group and intercalating phenazine group which stabilized complementary complexes. It was found that these oligonucleotide derivatives modify the DNA fragment in a specific way near the target complementary nucleotide sequences, and the DNA fragment can be cleaved at the alkylated nucleotides positions. Alkylating derivatives carrying phenazine groups were found to be the most efficient in reaction with the DNA fragment.
Sensory organ generation in the chicken inner ear: Contributions ofBone morphogenetic protein 4, Serrate1, andLunatic fringe (2000)

The chicken inner ear is a remarkably complex structure consisting of eight morphologically distinct sensory organs. Unraveling how these sensory organs are specified during development is key to understanding how such a complex structure is generated. Previously, we have shown that each sensory organ in the chicken inner ear arises independently in the rudimentary otocyst based on Bone morphogenetic protein 4 (Bmp4) expression. Here, we compare the expression of Bmp4 with two other putative sensory organ markers, Lunatic Fringe (L-fng) and chicken Serrate1 (Ser1), both of which are components of the Notch signaling pathway. L-fng and Ser1 expression domains were asymmetrically distributed in the otic cup. At this early stage, expression of L-fng is similar to Delta1 (Dl1), in an anteroventral domain apparently corresponding to the neurogenic region, while Ser1 is expressed at both the anterior and posterior poles. By the otocyst stage, the expression of both L-fng and Ser1 largely coincided in the medial region. All presumptive sensory organs, as identified by Bmp4 expression, arose within the broad L-fng- and Ser1-positive domain, indicating the existence of a sensory-competent region in the rudimentary otocyst. In addition, there is a qualitative difference in the levels of expression between L-fng and Ser1 such that L-fng expression was stronger in the ventral anterior, whereas Ser1 was stronger in the dorsal posterior region of this broad domain. This early difference in expression may presage the differences among sensory organs as they arise from this sensory competent zone. J. Comp. Neurol. 424:509–520, 2000. Published 2000 Wiley-Liss, Inc.
An Ultra-Low Voltage, Low-Noise, High Linearity 900-MHz Receiver With Digitally Calibrated In-Band Feed-Forward Interferer Cancellation in 65-nm CMOS (2011)

We present an ultra-low voltage, highly linear, low noise integrated CMOS receiver operating from a 0.6-V supply. The receiver incorporates programmable, in-band feed-forward interferer cancellation at the baseband to obtain high linearity and low noise operation at ultra-low supply voltages. Being able to reject adjacent channel or far-out blockers, the digitally calibrated interferer cancellation improves the IIP <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> and IIP <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> by more than 13 dB and 8 dB respectively with very little impact on the receiver noise figure. As such, it breaks the trade-off between linearity and noise figure, making it possible to use a high-gain RF front-end to achieve low noise figure without affecting the linearity of the ultra-low voltage baseband circuits. The 0.6-V 900-MHz direct-conversion receiver prototype integrates a differential LNA, RF transconductors, linear quadrature current driven passive mixers, feed-forward interferer cancellation circuits, baseband variable gain transimpedance amplifiers and second-order channel-select filters. It has a nominal conversion gain of 56.4 dB, noise figure of 5 dB, IIP <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> of -9.8 dBm and IIP <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> of 21.4 dBm. The receiver operates reliably from 0.55-0.65 V, consumes 26.4 mW and occupies an active area of 1.7 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in a 65-nm low-power CMOS process, of which the feed-forward interferer cancellation circuits consume 11.4 mW and occupies 0.43 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
Glycogen debranching enzyme: purification, antibody characterization, and immunoblot analyses of type III glycogen storage disease. (1987)

Type III glycogen storage disease is caused by a deficiency of glycogen debranching-enzyme activity. Many patients with this disease have both liver and muscle involvement, whereas others have only liver involvement without clinical or laboratory evidence of myopathy. To improve our understanding of the molecular basis of the disease, debranching enzyme was purified 238-fold from porcine skeletal muscle. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis the purified enzyme gave a single band with a relative molecular weight of 160,000 that migrated to the same position as purified rabbit-muscle debranching enzyme. Antiserum against porcine debranching enzyme was prepared in rabbit. The antiserum reacted against porcine debranching enzyme with a single precipitin line and demonstrated a reaction having complete identity to those of both the enzyme present in crude muscle and the enzyme present in liver extracts. Incubation of antiserum with purified porcine debranching enzyme inhibited almost all enzyme activity, whereas such treatment with preimmune serum had little effect. The antiserum also inhibited debranching-enzyme activity in crude liver extracts from both pigs and humans to the same extent as was observed in muscle. Immunoblot analysis probed with anti-porcine-muscle debranching-enzyme antiserum showed that the antiserum can detect debranching enzyme in both human muscle and human liver. The bands detected in human samples by the antiserum were the same size as the one detected in porcine muscle. Five patients with Type III and six patients with other types of glycogen storage disease were subjected to immunoblot analysis. Although anti-porcine antiserum detected specific bands in all liver and muscle samples from patients with other types of glycogen storage disease (Types I, II, and IX), the antiserum detected no cross-reactive material in any of the liver or muscle samples from patients with Type III glycogen storage disease. These data indicate (1) immunochemical similarity of debranching enzyme in liver and muscle and (2) that deficiency of debranching-enzyme activity in Type III glycogen storage disease is due to absence of debrancher protein in the patients that we studied.
Effect of Operating Conditions on Reverse Osmosis (RO) Membrane Performance (2023)

The aim of this research is to study the effect of high concentrations of salts, pressure and temperature on the performance of the RO membrane with time. Four different (Na2CO3) concentrations (5000, 15000, 25000 and 35000) ppm and various pressures such as (1, 3 and 5) bars at different temperatures of the feed solution (i.e., 25, 35 and 45) ◦C were used in this work. It was found that, as the concentration of salt and feed temperatures increase, the rejection of the salt decrease. While the salt rejection of the membranes increases with increase of transmembrane pressure.
A Double-Blind Study of Capacitively Coupled Electrical Stimulation as an Adjunct to Lumbar Spinal Fusions (1999)

Capacitively coupled stimulation is an effective adjunct to primary spine fusion, especially for patients with posterolateral fusion and those with internal fixation.
HYALURONIDASE AS AN ADJUVANT IN INSULIN SHOCK THERAPY (1956)

Extensive studies have been performed and reports published during the last few years concerning the biochemical, physiological, and therapeutic actions of hyaluronidase, the most important of the "spreading factors." Very few of these studies have been concerned with the use of hyaluronidase as an aid to insulin shock therapy with regard to decreasing the amount of insulin necessary, accelerating the evolution of comas and thus increasing the period of actual treatment, and avoiding its untoward effects, particularly the so-called after-shocks. Although the value of insulin shock therapy is still controversial, this therapy continues to be widely used in mental hospitals throughout the world for the management of schizophrenia and other psychiatric disorders. Therefore, every method leading to possible technical improvements in the administration of this therapy is welcome and should be given attention. Reports on the use of hyaluronidase in insulin shock therapy have been published by Straccia and Scheflein,