gebhart/patrepeval huggingface dataset:
Chemical pathology of homocysteine. II. Carcinogenesis and homocysteine thiolactone metabolism. (1994)

Abnormalities of methionine metabolism in malignancy include carcinogenicity of methionine deficiency, methionine auxotrophy of cultured malignant cells, deficient methylation of DNA, and aerobic glycolysis that is reversed by methionine. Cells from children with homocystinuria form an aggregated sulfated extracellular matrix and grow in a pattern similar to cultured malignant cells. Normal cells metabolize homocysteine thiolactone to sulfate, but malignant cells accumulate homocysteine thiolactone, which thiolates proteins and other cellular macromolecules. Thioretinamide, the amide of retinoic acid homocysteine thiolactone, and its cobalamin complex, thioretinaco, are antineoplastic and chemopreventive against carcinogenesis. Deficiency of these compounds in malignant cells is believed to increase conversion of methionine to homocysteine thiolactone and thioco, its cobalamin complex. These compounds are believed to participate in oxidative phosphorylation by formation of thioretinaco ozonide disulfonium complexes that are the active sites of adenosine triphosphate (ATP) binding in mitochondrial membranes. Hypothetical deficiency of thioretinaco may explain important metabolic abnormalities of malignant cells.
Studies of immunity in typhoid fever. Protection induced by killed oral antigens or by primary infection. (1971)

Adult male volunteers were orally vaccinated with two "killed" antityphoid preparations. The recommended doses of both vaccines resulted in serum antibody development in only a few of the subjects. When the dose of the monovalent preparation (Taboral) was doubled, serological responses occurred more frequently, with a rise in O agglutinins in nearly one-fifth of the subjects, in H agglutinins in approximately one-fourth, and in Vi antibodies in nearly half.When vaccinated volunteers were fed virulent typhoid organisms, disease occurred less frequently among those men vaccinated with Taboral at twice the recommended dose (38%) than among those not vaccinated (54%). This preparation did not confer protection at the recommended dose.Volunteers who had previously recovered from an induced typhoid infection received a further challenge with virulent organisms. These persons developed typhoid fever less frequently (23%) than individuals without prior typhoid exposure (30%).
Real-Time 3D Ladar Imaging (2006)

A prototype image processing system has recently been developed which generates, displays and analyzes three-dimensional ladar data in real time. It is based upon a suite of novel algorithms that transform raw ladar data into cleaned 3D images. These algorithms perform noise reduction, ground plane identification, detector response deconvolution and illumination pattern renormalization. The system also discriminates static from dynamic objects in a scene. In order to achieve real-time throughput, we have parallelized these algorithms on a Linux cluster. We demonstrate that multiprocessor software plus Blade hardware result in a compact, real-time imagery generation adjunct to an operating ladar.
Appearance-based place recognition for topological localization (2002)

This paper presents a new appearance-based place recognition system for topological localization. The method uses a panoramic vision system to sense the environment. Color images are classified in real-time based on nearest-neighbor learning, image histogram matching, and a simple voting scheme. The system has been evaluated with eight cross-sequence tests in four unmodified environments, three indoors and one outdoors. In all eight cases, the system successfully tracked the mobile robot's position. The system correctly classified between 87% and 98% of the input color images. For the remaining images, the system was either momentarily confused or uncertain, but never classified an image incorrectly.
Paradoxical Reactions to Benzodiazepines: Literature Review and Treatment Options (2004)

Benzodiazepines frequently are administered to patients to induce sedation. Paradoxical reactions to benzodiazepines, characterized by increased talkativeness, emotional release, excitement, and excessive movement, are relatively uncommon and occur in less than 1% of patients. The exact mechanism of paradoxical reactions remains unclear. Most cases are idiosyncratic; however, some evidence suggests that these reactions may occur secondary to a genetic link, history of alcohol abuse, or psychological disturbances. This review evaluates the numerous cases of paradoxical reactions to benzodiazepines in adult and pediatric patients that have been reported in the biomedical literature. It also explores the advantages and disadvantages of the various available treatment options.
A novel IP-routing lookup scheme and hardware architecture for multigigabit switching routers (1999)

One of the pertinent design issues for new generation IP routers is the route-lookup mechanism. For each incoming IP packet, the IP routing is required to perform a longest-prefix matching on the route lookup in order to determine the packet's next hop. This study presents a fast unicast route-lookup mechanism that only needs tiny SRAM and can be implemented using a hardware pipeline. The forwarding table, based on the proposed scheme, is small enough to fit into a faster SRAM with low cost. For example, a large routing table with 40000 routing entries can be compacted into a forwarding table of 450-470 kbytes costing less than US$30. Most route lookups need only one memory access; no lookup needs more than three memory accesses. When implemented using a hardware pipeline, the proposed mechanism can achieve one routing lookup every memory access. With current 10-ns SRAMs, this mechanism furnishes approximately 100/spl times/10/sup 6/ routing lookups/s, which is much faster than any current commercially available routing-lookup scheme.
Polarization-induced pulse spreading in birefringent optical fibers with zero differential group delay (1999)

The polarization properties of concatenations of trunks of birefringent fibers and elements with polarization-dependent losses are analyzed. We show both theoretically and experimentally that the concatenation can have zero differential group delay over a whole range of wavelengths but that a pulse propagating down the concatenation can still experience significant pulse spreading. In this example the two main methods used for characterizing polarization mode dispersion in optical fiber systems, i.e., Jones matrix eigenanalysis and the interferometric method, give different results. This counterintuitive example underlines the need for a careful assessment of the basic concepts related to polarization effects in the presence of polarization-dependent losses.
Doxorubicin acts via mitochondrial ROS to stimulate catabolism in C2C12 myotubes (2011)

Doxorubicin, a commonly prescribed chemotherapeutic agent, causes skeletal muscle wasting in cancer patients undergoing treatment and increases mitochondrial reactive oxygen species (ROS) production. ROS stimulate protein degradation in muscle by activating proteolytic systems that include caspase-3 and the ubiquitin-proteasome pathway. We hypothesized that doxorubicin causes skeletal muscle catabolism through ROS, causing upregulation of E3 ubiquitin ligases and caspase-3. We tested this hypothesis by exposing differentiated C2C12 myotubes to doxorubicin (0.2 μM). Doxorubicin decreased myotube width 48 h following exposure, along with a 40-50% reduction in myosin and sarcomeric actin. Cytosolic oxidant activity was elevated in myotubes 2 h following doxorubicin exposure. This increase in oxidants was followed by an increase in the E3 ubiquitin ligase atrogin-1/muscle atrophy F-box (MAFbx) and caspase-3. Treating myotubes with SS31 (opposes mitochondrial ROS) inhibited expression of ROS-sensitive atrogin-1/MAFbx and protected against doxorubicin-stimulated catabolism. These findings suggest doxorubicin acts via mitochondrial ROS to stimulate myotube atrophy.
Local control of the immune response in the liver (2000)

The physiological function of the liver--such as removal of pathogens and antigens from the blood, protein synthesis and metabolism--requires an immune response that is adapted to these tasks and is locally regulated. Pathogenic microorganisms must be efficiently eliminated while the large number of antigens derived from the gastrointestinal tract must be tolerized. From experimental observations it is evident that the liver favours the induction of tolerance rather than the induction of immunity. The liver probably not only is involved in transplantation tolerance but contributes as well to tolerance to orally ingested antigens (entering the liver with portal-venous blood) and to containment of systemic immune responses (antigen from the systemic circulation entering the liver with arterial blood). This review summarizes the experimental data that shed light on the molecular mechanisms and the cell populations of the liver involved in local immune regulation in the liver. Although hepatocytes constitute the major cell population of the liver, direct interaction of hepatocytes with leukocytes in the blood is unlikely. Sinusoidal endothelial cells, which line the hepatic sinusoids and separate hepatocytes from leukocytes in the sinusoidal lumen, and Kupffer cells, the resident macrophage population of the liver, can directly interact with passenger leukocytes. In the liver, clearance of antigen from the blood occurs mainly by sinusoidal endothelial cells through very efficient receptor-mediated endocytosis. Liver sinusoidal endothelial cells constitutively express all molecules necessary for antigen presentation (CD54, CD80, CD86, MHC class I and class II and CD40) and can function as antigen-presenting cells for CD4+ and CD8+ T cells. Thus, these cells probably contribute to hepatic immune surveillance by activation of effector T cells. Antigen-specific T-cell activation is influenced by the local microenvironment. This microenvironment is characterized by the physiological presence of bacterial constituents such as endotoxin and by the local release of immunosuppressive mediators such as interleukin-10, prostaglandin E2 and transforming growth factor-beta. Different hepatic cell populations may contribute in different ways to tolerance induction in the liver. In vitro experiments revealed that naive T cells are activated by resident sinusoidal endothelial cells but do not differentiate into effector T cells. These T cells show a cytokine profile and a functional phenotype that is compatible with the induction of tolerance. Besides sinusoidal endothelial cells, other cell populations of the liver, such as dendritic cells, Kupffer cells and perhaps also hepatocytes, may contribute to tolerance induction by deletion of T cells through induction of apoptosis.
Influenza Virus Vaccine Based on the Conserved Hemagglutinin Stalk Domain (2010)

Although highly effective in the general population when well matched to circulating influenza virus strains, current influenza vaccines are limited in their utility due to the narrow breadth of protection they provide. The strain specificity of vaccines presently in use mirrors the exquisite specificity of the neutralizing antibodies that they induce, that is, antibodies which bind to the highly variable globular head domain of hemagglutinin (HA). Herein, we describe the construction of a novel immunogen comprising the conserved influenza HA stalk domain and lacking the globular head. Vaccination of mice with this headless HA construct elicited immune sera with broader reactivity than those obtained from mice immunized with a full-length HA. Furthermore, the headless HA vaccine provided full protection against death and partial protection against disease following lethal viral challenge. Our results suggest that the response induced by headless HA vaccines is sufficiently potent to warrant their further development toward a universal influenza virus vaccine.
Effect of lactoferrin on proliferation and differentiation of the Jurkat human lymphoblastic T cell line. (1997)

The effect of human lactoferrin on the human lymphoblastic T cell line (Jurkat) was tested with regard to proliferation and differentiation. Lactoferrin enhanced cell proliferation in a serum-reduced (1% fetal calf serum) culture. The stimulatory effect of lactoferrin on proliferation depended on the degree of iron saturation but the amplitude of the effect was low, similar to that obtained in the presence of serum transferrin. The proliferation stimulatory effect of lactoferrin was not observed in the presence of 10% fetal calf serum (FCS) in the culture medium. These results suggest that Fe-lactoferrin can substitute for Fe-transferrin during the prolonged culture of cells in a low serum concentration. Iron-saturated lactoferrin was also shown to promote T cell differentiation. Jurkat cells, when exposed to iron-saturated lactoferrin in the presence of 10% FCS, gradually exhibited a decrease in the cell volume, cell surface density of CD71 antigen, the nuclear incorporation of [methyl-3H]thymidine, but an increase of the percentage of cell population in the G0/1 phase of the cell cycle. These modifications were accompanied by the appearance of CD4 antigen at the cell surface. Therefore, in the continuous presence of lactoferrin, proliferating cells slowly enter into quiescence state, undergoing cell differentiation.
Perspectives on oncologic imaging with radiolabeled antibodies (1997)

Both radioimmunodetection and PET provide new exciting opportunities for earlier and more sensitive functional imaging tests for cancer. In contrast to PET, antibody agents also can serve to qualify a patient as a candidate for an antibody-based therapy.
Nanotechnology for the biologist (2005)

Nanotechnology refers to research and technology development at the atomic, molecular, and macromolecular scale, leading to the controlled manipulation and study of structures and devices with length scales in the 1- to 100-nanometers range. Objects at this scale, such as "nanoparticles," take on novel properties and functions that differ markedly from those seen in the bulk scale. The small size, surface tailorability, improved solubility, and multifunctionality of nanoparticles open many new research avenues for biologists. The novel properties of nanomaterials offer the ability to interact with complex biological functions in new ways-operating at the very scale of biomolecules. This rapidly growing field allows cross-disciplinary researchers the opportunity to design and develop multifunctional nanoparticles that can target, diagnose, and treat diseases such as cancer. This article presents an overview of nanotechnology for the biologist and discusses "nanotech" strategies and constructs that have already demonstrated in vitro and in vivo efficacy.
Analyses of functions of an anti-PD-L1/TGFβR2 bispecific fusion protein (M7824) (2017)

M7824 (MSB0011359C) is a novel first-in-class bifunctional fusion protein consisting of a fully human IgG1 anti-PD-L1 monoclonal antibody (with structural similarities to avelumab) linked to the extracellular domain of two TGFβ receptor 2 (TGFβR2) molecules serving as a TGFβ Trap. Avelumab has demonstrated clinical activity in a range of human cancers and has been approved by the Food and Drug Administration for the therapy of Merkel cell and bladder carcinomas. Preclinical studies have shown this anti-PD-L1 is capable of mediating antibody-dependent cell-mediated cytotoxicity (ADCC). In the studies reported here, it is shown that M7824 is also capable of mediating ADCC of a wide range of human carcinoma cells <i>in vitro</i>, employing natural killer (NK) cells as effectors, albeit not as potent as anti-PD-L1 employing some tumor cells as targets. The addition of the IL-15 superagonist fusion protein complex ALT-803 enhanced the ADCC capacity of both anti-PD-L1 and M7824, and to levels that both agents now demonstrated similar levels of ADCC of tumor cells. TGFβ is a known immunosuppressive entity. Studies reported here show TGFβ1 induced reduction of several NK activation markers as well as reduction of endogenous NK lytic activity and NK-mediated ADCC of tumor cells. These phenomena could be reduced or mitigated, however, by M7824, but not by anti-PD-L1. M7824, but not anti-PD-L1, was also shown to reduce the immunosuppressive activity of regulatory T cells on human CD4<sup>+</sup> T-cell proliferation. These studies thus demonstrate the dual functionalities of M7824 and provide the rationale for its further clinical development.
Pseudomonas aeruginosa biofilms: role of the alginate exopolysaccharide (1995)

Pseudomonas aeruginosa synthesizes an exopolysaccharide called alginate in response to environmental conditions. Alginate serves to protect the bacteria from adversity in its surroundings and also enhances adhesion to solid surfaces. Transcription of the alginate biosynthetic genes is induced upon attachment to the substratum and this leads to increased alginate production. As a result, biofilms develop which are advantageous to the survival and growth of the bacteria. In certain circumstances, P. aeruginosa produces an alginate lyase enzyme which cleaves the polymer into short oligosaccharides. This negates the anchoring properties of the alginate and results in increased detachment of the bacteria away from the surface, allowing them to spread and colonize new sites. Thus, both alginate biosynthetic and degradative enzymes are important for the development, maintenance and spread of P. aeruginosa biofilms.
Irradiation of crystalline polyethylene (1962)

Abstract Measurements of crosslinking, unsaturation, and hydrogen evolution following electron bombardment are similar for bulk polyethylene and fast‐filtered single crystals. Slow‐filtered single crystals evidence a unique irradiation behavior. It is suggested that irradiation‐induced crosslinking is preferentially between the layers of polyethylene single crystals if the lamellae are in close contact. Following slow filtration the lamellae are separated while fast filtration of single crystal preparations brings the lamellae into contact. Moreover, it is possible that the bulk polymer is composed of folded molecules in compacted lamellae.
Thymosin β4 Is Cardioprotective after Myocardial Infarction (2007)

Heart disease is a leading cause of death in newborns and in adults. Efforts to promote cardiac repair by introduction or recruitment of exogenous stem cells hold promise but typically involve isolation and introduction of autologous or donor progenitor cells. We have found that the G-actin-sequestering peptide thymosin beta4 promotes myocardial and endothelial cell migration in the embryonic heart and retains this property in postnatal cardiomyocytes. Survival of embryonic and postnatal cardiomyocytes in culture was also enhanced by thymosin beta4. We found that thymosin beta4 formed a functional complex with PINCH and integrin-linked kinase (ILK), resulting in activation of the survival kinase Akt/PKB, which was necessary for thymosin beta4's effects on cardiomyocytes. After coronary artery ligation in mice, thymosin beta4 treatment resulted in upregulation of ILK and Akt activity in the heart, enhanced early myocyte survival, and improved cardiac function. These findings suggest that thymosin beta4 promotes cardiomyocyte and endothelial migration, survival, and repair and may be a novel therapeutic target in the setting of acute myocardial damage.
Improved Drug Delivery Using Microemulsions: Rationale, Recent Progress, and New Horizons (2001)

Microemulsions are excellent candidates as potential drug delivery systems because of their improved drug solubilization, long shelf life, and ease of preparation and administration. The formulation of microemulsions for pharmaceutical use requires a thorough understanding of the properties, uses, and limitations of microemulsions. Three distinct microemulsions--oil external, water external, and middle phase--can be used for drug delivery, depending upon the type of drug and the site of action. In this article, we present an examination of microemulsions as drug carrier systems, starting with general information and moving to a thorough review of the microemulsion literature, with a special section devoted to microemulsion-based gels.
Non‐muscle myosins in tumor progression, cancer cell invasion, and metastasis (2014)

The actin cytoskeleton, which regulates cell polarity, adhesion, and migration, can influence cancer progression, including initial acquisition of malignant properties by normal cells, invasion of adjacent tissues, and metastasis to distant sites. Actin-dependent molecular motors, myosins, play key roles in regulating tumor progression and metastasis. In this review, we examine how non-muscle myosins regulate neoplastic transformation and cancer cell migration and invasion. Members of the myosin superfamily can act as either enhancers or suppressors of tumor progression. This review summarizes the current state of knowledge on how mutations or epigenetic changes in myosin genes and changes in myosin expression may affect tumor progression and patient outcomes and discusses the proposed mechanisms linking myosin inactivation or upregulation to malignant phenotype, cancer cell migration, and metastasis.
Image watermarking using DCT domain constraints (2002)

Watermarking algorithms are used for image copyright protection. The algorithms proposed select certain blocks in the image based on a Gaussian network classifier. The pixel values of the selected blocks are modified such that their discrete cosine transform (DCT) coefficients fulfil a constraint imposed by the watermark code. Two different constraints are considered. The first approach consists of embedding a linear constraint among selected DCT coefficients and the second one defines circular detection regions in the DCT domain. A rule for generating the DCT parameters of distinct watermarks is provided. The watermarks embedded by the proposed algorithms are resistant to JPEG compression.
Production and utilization of acellular lung scaffolds in tissue engineering (2012)

Pulmonary disease is a worldwide public health problem that reduces the quality of life and increases the need for hospital admissions as well as the risk for premature death for those affected. For many patients, lung transplantation is the only chance for survival. Unfortunately, there is a significant shortage of lungs for transplantation and since the lung is the most likely organ to be damaged during procurement many lungs deemed unacceptable for transplantation are simply discarded. Rather than discarding these lungs they can be used to produce three-dimensional acellular (AC) natural lung scaffolds for the generation of engineered lung tissue. AC scaffolds are lungs whose original cells have been destroyed by exposure to detergents and physical methods of removing cells and cell debris. This creates a lung scaffold from the skeleton of the lungs themselves. The scaffolds are then used to support adult, stem or progenitor cells which can be grown into functional lung tissue. Recent studies show that engineered lung tissues are capable of surviving after in vivo transplantation and support limited gas exchange. In the future engineered lung tissue has the potential to be used in clinical applications to replace lung functions lost following injury or disease. This manuscript discusses recent advances in development and use of AC scaffolds to support engineering of lung tissues.