Production of Human Monoclonal Antibodies in SCID Mouse (1997)

Microbiology and ImmunologyVolume 41, Issue 12 p. 901-907 MinireviewFree Access Production of Human Monoclonal Antibodies in SCID Mouse Hai Nguyen, Hai Nguyen Department of Molecular and Cellular Pathology, University of Toronto, CanadaSearch for more papers by this authorJasbir Sandhu, Jasbir Sandhu Department of Surgery and Immunology, University of Toronto, CanadaSearch for more papers by this authorNobumichi Hozumi, Corresponding Author Nobumichi Hozumi Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Canada Research Institute for Biological Sciences, Science University of Tokyo, Noda, Chiba, 278 JapanAddress correspondence to Dr. Nobumichi Hozumi, Research Institute for Biological Sciences, Science University of Tokyo, 2669 Yamazaki, Noda, Chiba 278, Japan.Search for more papers by this author Hai Nguyen, Hai Nguyen Department of Molecular and Cellular Pathology, University of Toronto, CanadaSearch for more papers by this authorJasbir Sandhu, Jasbir Sandhu Department of Surgery and Immunology, University of Toronto, CanadaSearch for more papers by this authorNobumichi Hozumi, Corresponding Author Nobumichi Hozumi Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Canada Research Institute for Biological Sciences, Science University of Tokyo, Noda, Chiba, 278 JapanAddress correspondence to Dr. Nobumichi Hozumi, Research Institute for Biological Sciences, Science University of Tokyo, 2669 Yamazaki, Noda, Chiba 278, Japan.Search for more papers by this author First published: 14 November 2013 https://doi.org/10.1111/j.1348-0421.1997.tb01948.xCitations: 6AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abbreviations Ab antibody Ag antigen ASGM1 anti-asialo GM1 CFA complete Freund's adjuvant CFC colony-forming cell CSP circumsporozoite malaria parasite DNA-PK DNA-dependent protein kinase ELISA enzyme-linked immunosorbent assay GC germinal centre H immunoglobulin heavy chain HAMA response human anti-mouse antibody response HRP horseradish peroxidase HuMAb human monoclonal antibody Hu-PBL human peripheral blood lymphocyte Hu-PBL-SCID mouse SCID mouse engrafted with Hu-PBLs IFA incomplete Freund's adjuvant Ig immunoglobulin KLH keyhole limpet hemocyanin L immunoglobulin light chain MAb monoclonal antibody MoMAbs mouse monoclonal antibody PBL peripheral blood lymphocyte RSV respiratory syncytial virus scFv single-chain Fv SCID mouse severe combined immunodeficient mouse STn carbohydrate antigen V immunoglobulin variable region XGVHD xenogeneic graft versus host disease. References 1Abedi, M.R., Christensson, B., Islam, K.B., Hammarstrom, L., and Smith, C.I.E. 1992. Immunoglobuln production in severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood mononuclear cells. Eur. J. Immunol. 22: 823– 828. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar 2Aldrovandi, G.M., Fener, G., Gao, L., Jamieson, B., Kristeva, M., Chen, I., and Zack, J.A. 1993. The SCID-Hu mouse as a model for HIV infection. Nature 363: 732– 736. CrossrefCASPubMedWeb of Science®Google Scholar 3Blunt, T., Finnie, N.J., Taccioli, G.E., Smith, G., Demengeot, J., Gottlieb, T., Mizuta, R., Varghese, A., Alt, F.W., Jeggo, P., and Jackson, S.P. 1995. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair associated with the murine SCID mutation. Cell 80: 813– 823. CrossrefCASPubMedWeb of Science®Google Scholar 4Bonyhadl, M.L., Rabin, L., Sallmi, S., Brown, D.A., Kosek, J., McCune, J.M., and Kaneshima, H. 1993. HIV induces thymus depletion in vivo. Nature 363: 728– 732. CrossrefPubMedWeb of Science®Google Scholar 5Bosma, G.C., Custer, R.P., and Bosma, M.M.J. 1983. A severe combined immunodeficiency mutation in the mouse. Nature 301: 527– 530. CrossrefCASPubMedWeb of Science®Google Scholar 6Bosma, G.C., Fried, M., Custer, R.P., Carroll, A., Gilson, D.M., and Bosma, M.J. 1988. Evidence of finding lymphocytes in some (leaky) SCID mice. J. Exp. Med. 167: 1016– 1033. CrossrefPubMedWeb of Science®Google Scholar 7Boulianne, G., Hozumi, N., and Shulman, M. 1984. Production of functional chimeric mouse/human antibody. Nature 312: 643– 646. CrossrefCASPubMedWeb of Science®Google Scholar 8Boynton, E., Aubin, J., Hozumi, N., and Sandhu, J.S. 1996. Human osteoblasts survive and deposit new bone when human bone is implanted in SCID mice. Bone 18: 321– 326. CrossrefCASPubMedWeb of Science®Google Scholar 9Chanok, R.M., Parrott, R.H., Connors, M., Collins, P.L., and Murphy, B.R. 1992. Serious respiratory tract disease caused by respiratory syncytial virus: prospects for improved therapy and effective immunization. Pediatrics 90: 137– 143. PubMedWeb of Science®Google Scholar 10Dick, J.E. 1994. Future prospects for animal models created by transplanting human hematopoietic cells into immunodeficient mice. Res. Immunol. 145: 380– 384. CrossrefCASPubMedWeb of Science®Google Scholar 11Dorshkind, K., Pollack, S.B., Bosma, M.J., and Phillips, R.A. 1985. Natural killer (NK) cells are present in mice with severe combined immunodeficiency (SCID). J. Immunol. 134: 3798– 3801. PubMedWeb of Science®Google Scholar 12Duchosal, M.A., Eming, S.A., Fischer, P., Leturcq***, D., Barbas C.F., III, McConahey, P.J., Caothien, R.H., Thornton, G.B., Dixon, F.J., and Burton, D.R. 1992. Immunization of hu-PBL-SCID mice and the rescue of human monoclonal Fab fragments through combinatorial libraries. Nature 355: 258– 262. CrossrefCASPubMedWeb of Science®Google Scholar 13Elliott, M.J., Maini, R.N., Feldman, M., Kalden, J.R., Antoni, C., Smolen, J., Leeb, B., Breedweld, F.C., MacFarlane, J.D., Biji, H., and Woody, J.N. 1994. Randomized double-blind comparison of chimeric monoclonal antibody to tumour necrosis factor I (cA2) versus placebo in rheumatoid arthritis. Lancet 344: 1105– 1110. CrossrefCASPubMedWeb of Science®Google Scholar 14Fulop, G.M., and Phillips, R.A. 1986. Full reconstitution of the immune deficiency in SCID mice with normal stem cells required low-dose irradiation of the recipients. J. Immunol. 136: 4438– 4443. PubMedWeb of Science®Google Scholar 15Fulop, G.M., and Phillips, R.A. 1990. The SCID mutation in mice causes a general defect in DNA repair. Nature 347: 479– 482. CrossrefCASPubMedWeb of Science®Google Scholar 16Griffiths, A.D., Malmqvist, M., Marks, J.D., Bye, J., Embleton, M.J., McCafferty, J., Baier, M., Holliger, K., Gorick, B.D., Hughes-Jones, N., Hoogenboom, H.R., and Winter, G. 1993. Human anti-self antibodies with high specificity from phage display libraries. EMBO J. 12: 725– 734. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar 17Hartley, K.O., Gell, D., Smith, G.M., Zhang, H., Divechna, N., Connelly, M.A., Admon, A., Lees-Miller, S., Anderson, C.W., and Jackson, S.P. 1995. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell 82: 849– 856. CrossrefCASPubMedWeb of Science®Google Scholar 18Hendrickson, E.A., Schwartz, D.G., and Weaver, D.T. 1988. The SCID gene encodes a transacting factor that mediates the rejoining event of Ig gene rearrangement. Gene Develop. 2: 817– 829. CrossrefCASPubMedWeb of Science®Google Scholar 19Hozumi, N., Gorczynski, R., Peters, W., and Sandhu, J.S. 1994. A SCID mouse model for human immune response and disease. Res. Immunol. 145: 370– 379. CrossrefCASPubMedWeb of Science®Google Scholar 20Hozumi, N., and Sandhu, J. 1993. Recombinant antibody technology: its advent and advances. Cancer Inv. 11: 714– 723. CrossrefCASPubMedWeb of Science®Google Scholar 21Huse, W.D., Sastry, L., Iverson, S.A., Kang, A.S., Alting, M.M., Burton, D.R., Benkovic, S.J., and Lerner, R.A. 1989. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science 246: 1275– 1281. CrossrefCASPubMedWeb of Science®Google Scholar 22Jackson, J.R., Sathe, G., Rosenberg, M., and Sweet, R. 1995. In vitro maturation: improvement of a high affinity, neutralizing antibody against ***IL1/J. J. Immunol. 154: 3310– 3319. CASPubMedWeb of Science®Google Scholar 23Kamel-Reid, S., and Dicks, J.E. 1988. Engraftment of immune-deficient mice with human hematopoietic stem cells. Science 242: 1706– 1709. CrossrefCASPubMedWeb of Science®Google Scholar 24Kang, A.S., Barbas, C.F., Janda, K.D., Benkovic, S.J., and Lerner, R.A. 1991. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proc. Natl. Acad. Sci. U.S.A. 88: 4363– 4366. CrossrefCASPubMedWeb of Science®Google Scholar 25Kirchgessner, C.U., Patid, C.K., Evans, J.W., Cuomo, C.A., Fried, L.M., Carter, T., Oettinger, M.A., and Brown, J.M. 1995. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267: 1178– 1183. CrossrefCASPubMedWeb of Science®Google Scholar 26Kohler, G., and Milstein, C. 1975. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495– 497. CrossrefCASPubMedWeb of Science®Google Scholar 27Lapidot, T., Pflumio, F., Duodens, M., Murdoch, B., Williams, D., and Dick, J. 1992. Cytokine stimulation of multilineage human hematopoiesis from immature cells transplanted into SCID mice. Science 255: 1137– 1141. CrossrefCASPubMedWeb of Science®Google Scholar 28Lees-Miller, S.P., Godbout, R., Chan, D.W., Weinfeld, M., Day R.S.III, Barron, G.M., and Allalunis-Turner, J. 1995. Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line. Science 267: 1183– 1185. CrossrefCASPubMedWeb of Science®Google Scholar 29Lortenz, H.-M., Antoni, C., Valerius, T., Repp, R., Grunke, M., Schwerdtner, N., Nuslein, H., Woody, J., Kalden, J.R., and Manger, B. 1996. In vivo blockade of TNF-α by intravenous infusion of a chimeric monoclonal TNF-α antibody in patients with rheumatoid arthritis. J. Immunol. 156: 1646– 1653. PubMedWeb of Science®Google Scholar 30Mazingue, C., Cottrez, F., Auriault, C., Cesbron, J.-Y., and Capron, A. 1991. Obtention of a human primary humoral response against schistosome protective antigens in severe combined immunodeficiency mice after the transfer of human peripheral blood mononuclear cells. Eur. J. Immunol. 21: 1763– 1766. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar 31McCafferty, J., Griffiths, A.D., Winter, G., and Chiswell, D.J. 1990. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552– 554. CrossrefCASPubMedWeb of Science®Google Scholar 32McCune, J.M., Namikawa, R., Kaneshima, H., Shultz, L.D., Lieberman, M., and Weissman, I.L. 1988. The SCID-Hu mouse: murine model for the analysis of human hematolymphoid differentiation and function. Science 241: 1632– 1639. CrossrefCASPubMedWeb of Science®Google Scholar 33McHeyzer-Williams, M.G., McLean, M.J., Lalor, P.A., and Nossal, G.J.V. 1993. Antigen-driven B cell differentiation in vivo. J. Exp. Med. 178: 295– 307. CrossrefCASPubMedWeb of Science®Google Scholar 34Mendez, M.J., Green, L., Corvalan, J., Jia, X.-C., Maynard-Carrie, C., Yang, X., Gallo, M., Louie, D., Lee, D., Erickson, K., Luna, J., Roy, C., Abderrahim, H., Kirschenbaum, F., Noguchi, M., Smith, D., Fukushima, A., Hales, J., Finer, M., Davis, G., Zsebo, K., and Jakobovits, A. 1997. Functional transplant of megabase human immunoglobulin loci recapitulates human antibody response in mice. Nature Genet. 15: 146– 156. CrossrefCASPubMedWeb of Science®Google Scholar 35Miller, C., Stedra, J., Kelsue, G., and Gerny, J. 1995. Facultative role of germinal centers and T cells in the somatic diversification of IgVH genes. J. Exp. Med. 181: 1319– 1331. CrossrefCASPubMedWeb of Science®Google Scholar 36Mosier, D.E., Gulizia, R.J., Baird, S.M., and Wilson, D.B. 1988. Transfer of a functional human immune system to mice with severe combined immunodeficiency. Nature 335: 256– 259. CrossrefCASPubMedWeb of Science®Google Scholar 37Okazaki, K., Nishikawa, S., and Sakano, H. 1988. Aberrant immunoglobulin gene rearrangement in SCID mouse bone marrow cells. J. Immunol. 141: 1348– 1352. CASPubMedWeb of Science®Google Scholar 38Pennycook, J.L., Chang, Y., Celler, J., Phillips, R.A., and Wu, G.E. 1993. High frequency of normal DJH joints in B cell progenitors in severe combined immunodeficiency mice. J. Exp. Med. 178: 1007– 1016. CrossrefCASPubMedWeb of Science®Google Scholar 39Pluckthun, A. 1992. Mono- and bi-valent antibody fragments produced in Escherichia coli: engineering, folding and antigen binding. Immunol. Rev. 130: 151– 181. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar 40Sandhu, J.S., Boynton, E., Gorczynski, R., and Hozumi, N. 1996. The use of SCID mice in biotechnology and as a model for human disease. Critic. Rev. Biotech. 16: 95– 118. CrossrefCASPubMedWeb of Science®Google Scholar 41Sandhu, J.S., Clark, B., Boynton, E., Atkins, J., Messner, H., Keating, A., and Hozumi, N. 1996. Human hematopoiesis in SCID mice implanted with human adult cancellous bone. Blood 88: 1973– 1982. CASPubMedWeb of Science®Google Scholar 42Sandhu, J.S., Gorczynski, R., Shpitz, B., Gallinger, S., Nguyen, H.P., and Hozumi, N. 1995. Human model of xenogeneic graft versus host disease in SCID engrafted with human peripheral blood lymphocytes. Transplantation 60: 179– 184. CrossrefPubMedWeb of Science®Google Scholar 43Sandhu, J., Shpitz, B., Gallinger, S., and Hozumi, N. 1994. Human primary immune response in SCID mice engrafted with human peripheral blood lymphocytes. J. Immunol. 152: 3806– 3813. CASPubMedWeb of Science®Google Scholar 44Soderlind, E., Simonsson, A.C., and Borrebaeck, C.A.K. 1992. Phage display technology in antibody engineering: design of phagemid vectors and in vitro maturation systems. Immunol. Rev. 130: 109– 124. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar 45Verhoeyen, M., Milstein, C., and Winter, G. 1988. Reshaping human antibodies: grafting an antilysozyme activity. Science 239: 1534– 1536. CrossrefCASPubMedWeb of Science®Google Scholar 46Wertz, G.W., and Sullender, W.M. 1992. Approaches to immunization against respiratory syncytial virus. Biotechnology 20: 151– 176. CASPubMedGoogle Scholar Citing Literature Volume41, Issue12December 1997Pages 901-907 ReferencesRelatedInformation