gebhart/patrepeval huggingface dataset:
Haemin-binding proteins of Porphyromonas gingivalis W50 grown in a chemostat under haemin-limitation (1993)

Porphyromonas gingivalis W50 was grown in a chemostat at pH 7.3 under haemin-limitation and haemin-excess at a constant mean doubling time of 6.9 h. Outer membranes (OM) were extracted from whole cells using EDTA and compared by SDS-PAGE. Haemin-limited cells expressed novel outer membrane proteins (OMPs) of mol. mass 115, 113 and 19 kDa when samples were solubilized at 100 degrees C. A 46 kDa OMP was observed in haemin-excess cells but not in those from haemin-limited conditions. Tetramethylbenzidine (TMBZ) staining of gels, after OM solubilization at 20 degrees C, was used to detect haemin-binding proteins (HBPs). HBPs were observed only in OM from haemin-limited cells. The major HBP (mol. mass 32.4 kDa) corresponded to a similar sized Kenacid-blue-stained protein which was not observed in haemin-excess-derived OM. Haemin-limited cells and OM displayed a ladder-like series of Kenacid-blue-stained proteins. Lighter TMBZ-stained proteins of mol. mass 51, 53, 56 and 60 kDa, with mobilities corresponding to those of silver-stained LPS components, were observed in haemin-limited OM. No soluble HBPs were detected extracellularly. The greater number of HBPs expressed by cells grown under haemin-limitation may reflect an additional cell surface receptor system for haemin acquisition under low environmental levels of this essential cofactor.
A New Medium for the Isolation and Growth of Auxotrophic Mutants of the Blue-green Alga Anacystis nidulans (1973)

SUMMARY: A complete medium of defined composition has been developed for quantitative growth of wild-type and auxotrophic mutant strains of Anacystis nidulans. This medium has proved to be more satisfactory than other complex media (for example casein hydrolysate, yeast extract) for both the isolation and the growth of auxo-trophs. Rigorous control of the pH of complete and other supplemented media is essential for quantitative growth on agar. Four diagnostic media are described which each contain a different combination of the supplements used in the complete medium and facilitate the identification of the nutritional requirements of mutants. By using these media a number of auxotrophs have been isolated including five with novel phenotypes which require respectively (i) thiamine, (ii) p-aminobenzoic acid, (iii) a combination of pyruvate or acetate plus malate or succinate or fumarate, (iv) serine or glycine and (v) adenine.
The behaviour of 4-alkoxy methylene-2-phenyl-4H-oxazol-5-one and 4-dimethyl amino methylene-2-phenyl-4H-oxazol-5-one toward nitrogen nucleophiles under microwave heating (2005)

The reactivity of 4-ethoxymethylene-2-phenyloxazol-5(4H)-one and 4-dimethylamino methylene-2-phenyloxazol-5(4H)-one toward amines and active methylene reagents under microwave heating is reported. A new 3-aroylamino-4-pyridone synthesis is described.
A critique of Claude Rubinson's paper nulls, three - valued logic, and ambiguity in SQL (2008)

research-article A critique of Claude Rubinson's paper nulls, three - valued logic, and ambiguity in SQL: critiquing Date's critique Share on Author: C. J. Date Consultant, Healdsburg, CA Consultant, Healdsburg, CAView Profile Authors Info & Claims ACM SIGMOD RecordVolume 37Issue 3September 2008 pp 20–22https://doi.org/10.1145/1462571.1462574Online:30 September 2008Publication History 6citation303DownloadsMetricsTotal Citations6Total Downloads303Last 12 Months3Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Inadequacies and hazards of chymopapain injections as treatment for intervertebral disc disease (1975)

Chymopapain chemonucleolysis was performed on 100 patients with primary lumbar intervertebral disc disease. The results were compared with those of 174 patients who underwent laminotomy, foraminotomy, and discectomy. Primary lumbar intervertebral disc disease was arbitrarily divided into degenerative, complex, previous surgical, and simple disc syndromes. No difference was seen between chemonucleolysis and surgery in the first three divisions; between 55 percent and 60 per cent of patients responded successfully to treatment. In the simple disc division 89 per cent of the surgical and 60 per cent of the chemonucleolysis patients had successful results.
Polymorphism of the 3′ open reading frame of the virus associated with the acquired immune deficiency syndrome, human T-lymphotropic virus type III (1985)

The genome of the virus associated with the acquired immune deficiency syndrome (AIDS), human T-lymphotropic virus type III (HTLV-III), includes two open reading frames, not found in other retroviruses. One of these, designated 3' open reading frame (3'orf) is 648 base pairs (bp) in length, and overlaps with the 3' long terminal repeat (LTR) sequences. Sequences of additional HTLV-III clones were determined in order to estimate the level and location of variation within 3'orf, to gain some insight into the function of its protein product. Newly determined sequences are reported for 3'orf of two unintegrated clones of HTLV-III and three cDNA clones made from virion RNA derived from the same cell line infected with pooled blood samples of different patients with AIDS or AIDS-related complex symptoms (ARC). In addition, sequences for 3'orf were derived from an unintegrated viral clone derived from a different cell line infected with a distinct isolate from a single patient. These sequences are compared to those previously reported for six other viral clones. Sequences of 3'orf differ among clones by 1.1-10.4% bp and 2.4-17.0% of predicted amino acids. This represents significantly greater sequence variation than is found in the entire genome on average. Moreover, a functional proviral clone has a termination codon at amino acid residue 124 of this open reading frame. This raises questions concerning the structure, and regulation of expression of the protein encoded by 3'orf.
Characterization of aBacteroidesspecies from human intestine that degrades glycosaminoglycans (1998)

Polysaccharide lyases that can degrade glycosaminoglycans (GAGs) were identified in an anaerobic strain living in the human intestine. The strain was isolated from the stool of a healthy male and identified as Bacteroides sp. strain HJ-15. A detailed taxonomical study indicated the species is a strain of Bacteroides stercoris. The isolate was cultured and the polysaccharide lyase activity was partially purified. This enzyme preparation could act on GAGs containing either glucosamine or galactosamine suggesting the presence of both heparinases and chondroitinases. Various GAGs were incubated with the partially purified enzyme and the products formed were analyzed by strong anion-exchange high performance liquid chromatography and proton nuclear magnetic resonance spectroscopy. These studies demonstrated the presence of at least two types of polysaccharide lyases: heparin lyase and chondroitin sulfate lyase. The eliminative mechanism of these lyase enzymes was confirmed through the isolation of unsaturated disaccharide products. The heparin lyase acted on both heparin and acharan sulfate, a GAG recently isolated from Achatina fulica. The Bacteroides chondroitin lyase, acted on chondroitin sulfates A, B (dermatan sulfate), and C, resembling chondroitin lyase ABC. The presence of a GAG-degrading organism in human intestine may pose problems for the effective oral administration of GAG drugs.Key words: Bacteroides stercoris, glycosaminoglycan, nuclear magnetic resonance spectroscopy, polysaccharide lyase, heparinase, chondroitinase.
miRNAs as Non-Invasive Biomarkers for Lung Cancer Diagnosis (2014)

Lung cancer is a leading cause of cancer death and late diagnosis is one of the most important reasons for the high mortality rate. Circulating microRNAs (miRNAs) represent stable and reproducible markers for numerous solid tumors, including lung cancer, and have been hypothesized as non-invasive diagnostic markers. Serum, plasma or whole peripheral blood can be used as starting material, and several methodological approaches have been proposed to evaluate miRNA expression. The present review provides an in depth summary of current knowledge on circulating miRNAs in different types of biological samples used as diagnostic markers of lung cancer. We also evaluate the diagnostic accuracy of each miRNA or group of miRNAs in relation to the different housekeeping miRNAs used. Finally, the limitations and potential of miRNA analysis are discussed.
Split Stream Boilers for High-Temperature/High-Pressure Topping Steam Turbine Combined Cycles (1997)

Research and development work on high-temperature and high-pressure (up to 1500°F TIT and 4500 psia) topping steam turbines and associated steam generators for steam power plants as well as combined cycle plants is being carried forward by DOE, EPRI, and independent companies. Aeroderivative gas turbines and heavy-duty gas turbines both will require exhaust gas supplementary firing to achieve high throttle temperatures. This paper presents an analysis and examples of a split stream boiler arrangement for high-temperature and high-pressure topping steam turbine combined cycles. A portion of the gas turbine exhaust flow is run in parallel with a conventional heat recovery steam generator (HRSG). This side stream is supplementary fired opposed to the current practice of full exhaust flow firing. Chemical fuel gas recuperation can be incorporated in the side stream as an option. A significant combined cycle efficiency gain of 2 to 4 percentage points can be realized using this split stream approach. Calculations and graphs show how the DOE goal of 60 percent combined cycle efficiency burning natural gas fuel can be exceeded. The boiler concept is equally applicable to the integrated coal gas fuel combined cycle (IGCC).
High-efficiency, high step-up DC-DC converters (2003)

Many applications call for high step-up DC-DC converters that do not require isolation. Some DC-DC converters can provide high step-up voltage gain, but with the penalty of either an extreme duty ratio or a large amount of circulating energy. DC-DC converters with coupled inductors can provide high voltage gain, but their efficiency is degraded by the losses associated with leakage inductors. Converters with active clamps recycle the leakage energy at the price of increasing topology complexity. A family of high-efficiency, high step-up DC-DC converters with simple topologies is proposed in this paper. The proposed converters, which use diodes and coupled windings instead of active switches to realize functions similar to those of active clamps, perform better than their active-clamp counterparts. High efficiency is achieved because the leakage energy is recycled and the output rectifier reverse-recovery problem is alleviated.
Pulsed laser desorption of biological molecules in supersonic beam mass spectrometry with resonant two-photon ionization detection (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPulsed laser desorption of biological molecules in supersonic beam mass spectrometry with resonant two-photon ionization detectionRoger. Tembreull and David M. LubmanCite this: Anal. Chem. 1987, 59, 8, 1082–1088Publication Date (Print):April 15, 1987Publication History Published online1 May 2002Published inissue 15 April 1987https://pubs.acs.org/doi/10.1021/ac00135a004https://doi.org/10.1021/ac00135a004research-articleACS PublicationsRequest reuse permissionsArticle Views237Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Organic reactions in chiral micelles: 7. The structural effects on the asymmetric oxidation of prochiral sulfides in chiral micelles (1990)

Abstract A variety of phenyl alkyl sulfides were oxidized enantioselectively with NaIO 4 in chiral micellar systems formed from eight chiral surfactants, to give optical active sulfoxides. The enantiomer excesses ranged from 1.6 to 15.0%. To understand the mechanistic detail of this asymmetric oxidation in chiral micelle, the effects of structure both in substrates and surfactants on the optical yield of the oxidation were studied and discussed. Generally, increasing the alkyl chain length both in surfactants and in substrates enhances the optical yield, also the surfactant with hydroxy group at its appropriate position gives better enantioselectivity, suggesting the enzymic characteristics of the chiral micelle.
In Vitro Modulation of Microglia Motility by Glioma Cells Is Mediated by Hepatocyte Growth Factor/Scatter Factor (1999)

HGF/SF, which plays a role in glioma motility and mitogenesis, may also act as a chemokine for microglia and may be responsible for the microglia infiltration in malignant gliomas. This active recruitment of microglia may play an important role in glioma biology.
Regulation of basement membrane heparan sulfate proteoglycan, perlecan, gene expression in glomerular epithelial cells by high glucose medium (1996)

Proteinuria in diabetic nephropathy has been correlated with reduction in heparan sulfate proteoglycan (HSPG) content of the glomerular basement membrane. We have previously shown that the underlying mechanism probably involves reduction in the synthesis by glomerular epithelial cells. In this study we explored whether high glucose medium regulates basement membrane HSPG gene expression. Northern analysis demonstrated that rat glomerular epithelial cells in vitro constitutively express mRNA for basement membrane HSPG, similar to that observed in rat kidney glomerulus. RNase protection assay showed that incubation of glomerular epithelial cells with 30 mM glucose for 24 h and 7 days resulted in reduction in HSPG mRNA abundance. The decrease in mRNA abundance correlated with reduction in the synthesis of 35 SO4-labeled basement membrane HSPG as measured by immunoprecipitation. Reduction in synthesis of HSPG could not be entirely accounted for by decrease in mRNA abundance, suggesting both transcriptional and posttranscriptional mechanisms may be involved in reduction of glomerular basement membrane HSPG synthesis by glomerular epithelial cells in diabetic nephropathy. © 1996 Wiley-Liss, Inc.
The human immunoglobulin pseudo-gamma IGHGP gene shows no major structural defect (1988)

International audience
Bactericidal Effect of 0.95-mW Helium-Neon and 5-mW Indium-Gallium-Aluminum-Phosphate Laser Irradiation at Exposure Times of 30, 60, and 120 Seconds on Photosensitized Staphylococcus aureus and Pseudomonas aeruginosa In Vitro (1999)

Laser-induced microbial killing of photosensitized organisms could have clinical applications in the treatment of infected skin lesions, pending in vivo studies.
Cords, channels, corridors and conduits: critical architectural elements facilitating cell interactions in the lymph node cortex (1997)

The lymph node cortex is a critical site for encounter between recirculating T cells and their specific antigens. Due to its extreme plasticity, little is understood of the underlying functional unit of the lymph node cortex, the paracortical cord. The idealized paracortical cord (approximately 100 microns by 1000 microns) stretches from a medullary cord to the base of a B-cell follicle. In cross-section, a cord can be visualized as a set of nested cylinders consisting of spaces bounded by cells. The spaces are: i) the lumen of the high endothelial venule (HEV), ii) perivenular channels-narrow potential spaces (0.1 micron) tightly encircling the HEV, iii) corridors-broad spaces (10-15 microns) constituting the majority of the parenchyma, and iv) the cortical sinus. In addition to these spaces for cell traffic, the conduit (fifth space) is a special delivery system for the transit of soluble factors to the HEV and emigrating lymphocytes. The cellular barriers between these spaces are high endothelium, fibroblastic reticular cells, or sinus-lining cells. This review describes the spaces of the paracortical cord and their cellular boundaries, outlines the movement of cells and fluids through these spaces, and discusses how this anatomy affects the efficiency of surveillance by T cells.
Residue size at position 87 of cytochrome P450 BM‐3 determines its stereoselectivity in propylbenzene and 3‐chlorostyrene oxidation (2001)

We report here oxidation of propylbenzene and 3‐chlorostyrene by wild‐type cytochrome P450 BM‐3 with high turnover (479 nmol 1‐phenyl‐1‐propanol/min/nmol P450 and 300 nmol 3‐chlorostyrene oxide/min/nmol P450). Furthermore, the residue size at position 87 of P450 BM‐3 was found to play critical roles in determining stereoselectivity in oxidation of propylbenzene and 3‐chlorostyrene. Replacement of Phe87 with Val, Ala and Gly resulted in decreases in optical purity of produced ( R )‐(+)‐1‐phenyl‐1‐propanol from 90.0 to 37.4, 26.0 and −15.6% e.e ., respectively, and in increases in those of produced ( R )‐(+)‐3‐chlorostyrene oxide from −61.0 to −38.0, 67.0 and 94.6% e.e ., respectively.
Combined antimicrotubule activity of estramustine and taxol in human prostatic carcinoma cell lines. (1992)

Estramustine (EM) and taxol, two antimicrotubule agents with distinct and apparently opposing mechanisms of action, were found to be effective in combination in the preclinical treatment of EM-resistant and sensitive, wild-type human prostatic carcinoma cell lines. Estramustine combined with 1 nM taxol (concentration 100-fold less than that measured in plasma of patients treated with taxol) produced greater than additive effects on the inhibition of cell survival of both wild-type and EM-resistant cells. When taxol was used with another microtubule-destabilizing drug, vinblastine, no significantly increased cytotoxicity was observed. Other effects on wild-type and EM-resistant cells produced by the combination of EM and taxol included (a) an increased proportion of the cells in the S phase of the cell cycle; (b) no mitotic block; and (c) an increase in the percentage of micronucleated cells from a control value of less than 1% to greater than 20% after drug treatment. Immunofluorescent microscopic analysis of the effect of this drug combination on the mitotic spindle apparatus revealed specific examples of aberrant mitotic figures, including multiple asters, cells with two distinct spindles, and tripolar spindles able to traverse mitosis and complete cytokinesis. These data provide supportive preclinical evidence for the potential development of an EM/taxol combination clinical regimen either for prostate or other cancers.
Wideband extension of narrowband speech for enhancement and coding (2000)

Most existing telephone networks transmit narrowband coded speech which has been bandlimited to 4 kHz. Compared with normal speech, this speech has a muffled quality and reduced intelligibility, which is particularly noticeable in sounds such as /s/, /f/ and /sh/. Speech which has been bandlimited to 8 kHz is often coded for this reason, but this requires an increase in the bit rate. Wideband enhancement is a scheme that adds a synthesized highband signal to narrowband speech to produce a higher quality wideband speech signal. The synthesized highband signal is based entirely on information contained in the narrowband speech, and is thus achieved at zero increase in the bit rate from a coding perspective. Wideband enhancement can function as a post-processor to any narrowband telephone receiver, or alternatively it can be combined with any narrowband speech coder to produce a very low bit rate wideband speech coder. Applications include higher quality mobile, teleconferencing, and internet telephony. This thesis examines in detail each component of the wideband enhancement scheme: highband excitation synthesis, highband envelope estimation, and narrowband-highband envelope continuity. Objective and subjective test measures are formulated to assess existing and new methods for all components, and the likely limitations to the performance of wideband enhancement are also investigated. A new method for highband excitation synthesis is proposed that uses a combination of sinusoidal transform coding-based excitation and random excitation. Several new techniques for highband spectral envelope estimation are also developed. The performance of these techniques is shown to be approaching the limit likely to be achieved. Subjective tests demonstrate that wideband speech synthesized using these techniques has higher quality than the input narrowband speech. Finally, a new paradigm for very low bit rate wideband speech coding is presented in which the quality of the wideband enhancement scheme is improved further by allocating a very small bitstream for highband envelope and gain coding. Thus, this thesis demonstrates that wideband speech can be communicated at or near the bit rate of a narrowband speech coder.
A human serum mannose-binding protein inhibits in vitro infection by the human immunodeficiency virus. (1989)

In vitro infection by the human immunodeficiency virus (HIV) of CD4+ H9 lymphoblasts is inhibited by a mannose-binding protein (MBP) purified from human serum. In addition, MBP is able to selectively bind to HIV-infected H9 cells and HIV-infected cells from the monocyte cell line U937. These results indicate MBP most likely recognizes high mannose glycans known to be present on gp120 in the domain that is recognized by CD4 and thereby inhibits viral entry to susceptible cells. In support of this contention, recombinant gp120 binds directly to MBP; the binding is saturable, mannan inhibitable, removed by N-glycanase treatment, and dependent on divalent cations.