gebhart/patrepeval huggingface dataset:
Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device (2010)

Microtechnology offers great prospects for cellular research by enabling controlled experimental conditions that cannot be achieved by traditional methods. This study demonstrates the use of a microfluidic platform for long-term cultivation (3 weeks) of human mesenchymal stem-like cells (MSCs), a cell population of high interest for tissue engineering. The typical high motility of the MSCs required a strategy for preventing cells from inhabiting the feeding channels and thus interfere with a steady perfusion of medium to the cell cultivation chamber. Hence, a straightforward and long-term patterning method was developed and implemented for reliable cell positioning within the device. This method was based on the modification of a polystyrene substrate into cell supportive and non-supportive regions by the use of selective oxygen plasma treatment and the triblock copolymer Pluronic. Also, a novel and size-effective "flip-chip" set-up for operating the devices was invented. Successful and reproducible adipogenic and osteogenic differentiation of MSCs in the device was demonstrated, verifying that an adequate long-term microfluidic cultivation environment was obtained. Strengths of the experimental protocol include ease of fabrication and maintenance (gravity driven), good cell performance (viability/differentiation), as well as the possibility of exposing the culture to heterogeneous laminar flow for experimental purposes.
A Unique Fatty Acid from Limnanthes douglasii Seed Oil: The C22 Diene (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Unique Fatty Acid from Limnanthes douglasii Seed Oil: The C22 DieneM. O. BAGBY, C. R. SMITH Jr., T. K. MIWA, R. L. LOHMAR, and I. A. WOLFFCite this: J. Org. Chem. 1961, 26, 4, 1261–1265Publication Date (Print):April 1, 1961Publication History Published online1 May 2002Published inissue 1 April 1961https://pubs.acs.org/doi/10.1021/jo01063a067https://doi.org/10.1021/jo01063a067research-articleACS PublicationsRequest reuse permissionsArticle Views80Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effect of Binary Additive Combinations on Solution Crystal Growth of the Poorly Water-Soluble Drug, Ritonavir (2012)

Combinations of additives (polymers and surfactants) are often used in pharmaceutical products to improve the delivery of poorly water-soluble active pharmaceutical ingredients (API). Additive interactions have not been widely studied and may promote or inhibit crystallization (nucleation and crystal growth) in an unpredictable manner, which in turn has an impact on the extent and duration of supersaturation. In this study, the effect of a series of polymer/polymer and polymer/surfactant combinations on crystal growth inhibition was investigated. Surprisingly, the majority of the polymer/polymer combinations investigated had a synergistic effect on crystal growth inhibition. The effectiveness of the polymer/polymer combinations was ascribed to the formation of interpolymer complexes through hydrophobic interactions that adsorb and interact favorably with the crystallizing solute and/or, interaction of individual polymers at different adsorption sites. The acceleration of crystal growth in the presence of polymer/surfactant combinations was attributed to weakened interactions between the polymer and the surface of the crystallizing solute brought about by the presence of surfactant molecules. Based on these observations, careful evaluation of the impact of combinations of additives on crystallization behavior is recommended in order to optimize the performance of supersaturating dosage forms.
Long-term in vivo transmission of alpha-particle-induced chromosomal instability in murine haemopoietic cells (1996)

We have previously reported non-clonal cytogenetic aberrations in the clonal descendants of murine haemopoietic stem cells irradiated in vitro with alpha-particles. The data are consistent with a transmissible chromosomal instability induced in a stem cell resulting in a diversity of aberrations in its clonal progeny many cell divisions later. To determine whether this instability is transmissible in vivo, bone marrow cells obtained from the male mouse have been irradiated with alpha-particles in vitro and transplanted into female recipients. In the repopulated haemopoietic system we have observed persisting chromosomal instability up to 1 year post-transplantation. The findings demonstrate a long-lived effect of the alpha-particle-induced lesion in the donor repopulating stem cells.
Involvement of cis‐acting repressive element(s) in the 3′‐untranslated region of human connective tissue growth factor gene (1999)

To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR.
A Document Image Model and Estimation Algorithm for Optimized JPEG Decompression (2009)

The JPEG standard is one of the most prevalent image compression schemes in use today. While JPEG was designed for use with natural images, it is also widely used for the encoding of raster documents. Unfortunately, JPEG's characteristic blocking and ringing artifacts can severely degrade the quality of text and graphics in complex documents. We propose a JPEG decompression algorithm which is designed to produce substantially higher quality images from the same standard JPEG encodings. The method works by incorporating a document image model into the decoding process which accounts for the wide variety of content in modern complex color documents. The method works by first segmenting the JPEG encoded document into regions corresponding to background, text, and picture content. The regions corresponding to text and background are then decoded using maximum a posteriori (MAP) estimation. Most importantly, the MAP reconstruction of the text regions uses a model which accounts for the spatial characteristics of text and graphics. Our experimental comparisons to the baseline JPEG decoding as well as to three other decoding schemes, demonstrate that our method substantially improves the quality of decoded images, both visually and as measured by PSNR.
Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: An overview of enabling science and potential applications (2005)

The application of probiotics and prebiotics to the manipulation of the microbial ecology of the human colon has recently seen many scientific advances. The sequencing of probiotic genomes is providing a wealth of new information on the biology of these microorganisms. In addition, we are learning more about the interactions of probiotics with human cells and with pathogenic bacteria. An alternative means of modulating the colonic microbial community is by the use of prebiotic oligosaccharides. Increasing knowledge of the metabolism of prebiotics by probiotics is allowing us to consider specifically targeting such dietary intervention tools at specific population groups and specific disease states.
Multimedia traffic analysis using CHITRA95 (1995)

We describe how to investigate collections of trace data representing network delivery of multimedia information with CHITRA95, a tool that allows a user to visualize, query, statistically analyze and test, transform, and model collections of trace data. CHITRA95 is applied to characterize World Wide Web (WWW) traffic from three workloads: students in a classroom of network-connected workstations, graduate students browsing the Web, undergraduates browsing educational and other materials, as well as traffic on a courseware repository server. We explore the inter-access time of files on a server (i.e., recency), the hit rate from a proxy server cache, and the distributions of file sizes and media types requested. The traffic study also yields statistics on the effectiveness of caching to improve transfer rates. In contrast to past WWW traffic studies, we analyze client as well as server traffic; we compare three workloads rather than drawing conclusions from one workload; and we analyze tcpdump logs to calculate the performance improvement in throughput that an end user sees due to caching.
Model-based teeth reconstruction (2016)

In recent years, sophisticated image-based reconstruction methods for the human face have been developed. These methods capture highly detailed static and dynamic geometry of the whole face, or specific models of face regions, such as hair, eyes or eye lids. Unfortunately, image-based methods to capture the mouth cavity in general, and the teeth in particular, have received very little attention. The accurate rendering of teeth, however, is crucial for the realistic display of facial expressions, and currently high quality face animations resort to tooth row models created by tedious manual work. In dentistry, special intra-oral scanners for teeth were developed, but they are invasive, expensive, cumbersome to use, and not readily available. In this paper, we therefore present the first approach for non-invasive reconstruction of an entire person-specific tooth row from just a sparse set of photographs of the mouth region. The basis of our approach is a new parametric tooth row prior learned from high quality dental scans. A new model-based reconstruction approach fits teeth to the photographs such that visible teeth are accurately matched and occluded teeth plausibly synthesized. Our approach seamlessly integrates into photogrammetric multi-camera reconstruction setups for entire faces, but also enables high quality teeth modeling from normal uncalibrated photographs and even short videos captured with a mobile phone.
Complement-induced vesiculation and exposure of membrane prothrombinase sites in platelets of paroxysmal nocturnal hemoglobinuria (1993)

Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired stem-cell disorder in which the glycolipid-anchored membrane proteins, including the cell-surface complement inhibitors, CD55 and CD59, are partially or completely deleted from the plasma membranes of mature blood cells. To gain insight into the pathogenesis of thrombosis that is frequently observed in this disorder, the procoagulant responses of PNH platelets exposed to the human terminal complement proteins C5b-9 were investigated. C5b-9 complexes were assembled on gel-filtered platelets by incubation with purified C5b6, C7, C9, and limiting amounts of C8. Platelet microparticle formation and exposure of plasma membrane-binding sites for coagulation factor Va were then analyzed by flow cytometry. PNH platelets exhibiting undetectable levels of surface CD59 antigen showed an approximately 10-fold increase in sensitivity to C5b-9-stimulated expression of membrane-binding sites for factor Va when compared with platelets from normal controls. Expression of catalytic surface for the prothrombinase complex (VaXa) paralleled the exposure of factor Va-binding sites; the rate of prothrombin conversion by C5b-9-treated PNH platelets exceeded that of C5b-9-treated normal controls by approximately 10-fold at the maximal input of C8 tested (500 ng/mL). These data indicate that PNH platelets deficient in plasma membrane CD59 antigen are exquisitely sensitive to C5b-9-induced expression of prothrombinase activity, and suggest that the tendency toward thrombosis in these patients may be due, at least in part, to the deletion of this complement inhibitor from the platelet plasma membrane.
Identification of a Gene that Complements an Arabidopsis Mutant Deficient in Chloroplast [omega]6 Desaturase Activity (1994)

Membrane lipids of the fad6 (formerly fadC) mutant of Arabidopsis, which is deficient in chloroplast omega 6 desaturase activity, have increased levels of monounsaturated fatty acids and are deficient in trienoic fatty acids. A putative fad6 cDNA clone was isolated by probing a cDNA library with a degenerate oligonucleotide based on a conserved region within known omega 3 desaturase genes. Expression of the cDNA in transgenic plants of a fad6 mutant restored normal levels of all fatty acids. When used as a hybridization probe, the cDNA identified a restriction fragment-length polymorphism that co-segregated with the fad6 mutation. Thus, on the basis of a genetic complementation test and genetic map position, the fad6 gene is encoded by the cDNA. The cDNA encoded a 418-amino acid polypeptide of 47,727 D that displayed a high degree of sequence similarity to a delta 12 desaturase from the cyanobacterium Synechocystis. The fad6 gene exhibited less sequence homology to any known higher plant desaturase, including an endoplasmic reticulum-localized omega 6 desaturase corresponding to the Arabidopsis fad2 gene.
Vaccinia virus-infected cells release a novel polypeptide functionally related to transforming and epidermal growth factors. (1985)

The recent discovery, that a vaccinia virus (VV) gene encodes a polypeptide with structural homology to transforming growth factor (TGF-alpha) and epidermal growth factor (EGF), led us to look for a virus-induced protein with the predicted biological activity. The supernatants of VV-infected cell cultures were found to contain an acid stable Mr 25,000 polypeptide that competes with EGF for binding to EGF membrane receptors. This VV-induced growth factor (VGF) like EGF and TGF-alpha is mitogenic and stimulates anchorage-independent cell growth in the presence of TGF-beta. However, VGF did not cross-react in a radioimmunoassay specific for small and large forms of TGF-alpha and exhibited minimal cross-reactivity with antisera to EGF. VGF was detectable in the culture medium within 2 hr, and maximal amounts were present 12 hr after infection. The level of VGF was proportional to the multiplicity of VV used. Inhibition of viral DNA synthesis enhanced VGF production, consistent with the hypothesis that VGF is an early gene product encoded by VV. The demonstration of a novel growth factor, released from cells infected with VV, may have important implications regarding the nature of virus-host interactions.
Photothermal imaging of nanoparticles and cells (2005)

This review summarizes the findings of recent applications of time-domain far-field photothermal (PT) technique to the detection and imaging of nanoscale absorbing particles. This two-beam (pump-probe) technique is based on time-resolved PT visualization of laser-induced thermal effects around nanoparticles. Imaging is accomplished, after an adjustable time delay after the pump laser pulse, with a second probe beam that senses the nanotarget. Using a tunable optical parametric oscillator laser (wavelength, 420 to 570 nm; energy, 0.1-300 /spl mu/J; pulse width, 8 ns) as the pump laser and a Raman shifter (639 nm, 10 nJ, 13 ns) as the probe laser, with a tunable delay of 0 to 5 000 ns of the probe pulse relative to the pump pulse, this approach has demonstrated the capability to visualize nanoscale gold particles (2 to 250 nm) alone and in cells, liposomes (30 to 90 nm), neutral red-stained particles (30 to 500 nm), and polystyrene beads. Different applications of the time-resolved PT technique are discussed, including imaging of absorbing cellular nanostructures and optimization of selective killing of cancer cells and bacteria.
Electrophilic Affibodies Forming Covalent Bonds to Protein Targets (2009)

Antibody affinity limits sensitivity of detection in many areas of biology and medicine. High affinity usually depends on achieving the optimal combination of the natural 20 amino acids in the antibody binding site. Here, we investigate the effect on recognition of protein targets of placing an unnatural electrophile adjacent to the target binding site. We positioned a weak electrophile, acrylamide, near the binding site between an affibody, a non-immunoglobulin binding scaffold, and its protein target. The proximity between cysteine, lysine, or histidine on the target protein drove covalent bond formation to the electrophile on the affibody. Covalent bonds did not form to a non-interacting point mutant of the target, and there was minimal cross-reactivity with serum, cell lysate, or when imaging at the cell surface. Electrophilic affibodies showed more stable protein imaging at the surface of mammalian cells, and the sensitivity of protein detection in an immunoassay improved by two orders of magnitude. Thus electrophilic affibodies combined good specificity with improved detection of protein targets.
Chondroitin Sulfate Perlecan Enhances Collagen Fibril Formation (2006)

Inactivation of the perlecan gene leads to perinatal lethal chondrodysplasia. The similarity to the phenotypes of the Col2A1 knock-out and the disproportionate micromelia mutation suggests perlecan involvement in cartilage collagen matrix assembly. We now present a mechanism for the defect in collagen type II fibril assembly by perlecan-null chondrocytes. Cartilage perlecan is a heparin sulfate or a mixed heparan sulfate/chondroitin sulfate proteoglycan. The latter form binds collagen and accelerates fibril formation in vitro, with more defined fibril morphology and increased fibril diameters produced in the presence of perlecan. Interestingly, the enhancement of collagen fibril formation is independent on the core protein and is mimicked by chondroitin sulfate E but neither by chondroitin sulfate D nor dextran sulfate. Furthermore, perlecan chondroitin sulfate contains the 4,6-disulfated disaccharides typical for chondroitin sulfate E. Indeed, purified glycosaminoglycans from perlecan-enriched fractions of cartilage extracts contain elevated levels of 4,6-disulfated chondroitin sulfate disaccharides and enhance collagen fibril formation. The effect on collagen assembly is proportional to the content of the 4,6-disulfated disaccharide in the different cartilage extracts, with growth plate cartilage glycosaminoglycan being the most efficient enhancer. These findings demonstrate a role for perlecan chondroitin sulfate side chains in cartilage extracellular matrix assembly and provide an explanation for the perlecan-null chondrodysplasia.
Affymetrix GeneChip® system: moving from research to the clinic (2006)

Affymetrix Inc.'s GeneChip technology has become the industry standard in microarray-based research. Due to the high density of content per array that can be achieved today (an industry leading 6.5 million features) the GeneChips can be used for high-throughput mutation detection, single nucleotide polymorphism genotyping, expression profiling and detection of chromosomal aberrations. This in turn opens the way for clinical applications in genetics, cytogenetics, pharmacogenetics, oncology and pathogen recognition. Establishing standards is a central issue in improving data quality and, in combination with automatic, easy-to-interpret reports, will form the basis of the clinical applicability. Future pricing policies and the resolving of ethical considerations will also dictate the technology's full translation from research into clinical laboratories.
Slot antenna design for wireless communications systems (2007)

In this paper, we present the practical considerations of designing antennas for cellular handsets as well as several antenna miniaturization techniques that can be used to reduce the size of planar slot antennas. Examples of miniaturized slot antennas that are 15 to 20 times smaller than traditional dipole or patch antennas are presented and discussed. Miniaturization techniques that can be used to reduce the size of cavity-backed slot antennas, by as much as 80%, are also presented. It is shown that, even with the very small cavity- dimensions, the antennas still demonstrate front-to-back ratios of 4-7 dB. Moreover, an example of a miniaturized on-chip slot antenna integrated with a low noise amplifier on a complementary metal oxide semiconductor (CMOS) chip is also presented and discussed. Finally a particular antenna configuration, optimized to possess good performance characteristics for a multiple input multiple output (MIMO) antenna module in small real estates, is presented and discussed.
Negative regulation of the major histocompatibility complex class I enhancer in adenovirus type 12-transformed cells via a retinoic acid response element (1992)

In cells transformed by the highly oncogenic adenovirus type 12 (Ad12), the viral E1A proteins mediate transcriptional repression of the major histocompatibility class I genes. In contrast, class I transcription is not reduced in cells transformed by the nononcogenic Ad5. The decreased rate of class I transcription is, at least in part, the result of a reduced major histocompatibility complex class I enhancer activity in Ad12-transformed cells and correlates with an increase in the levels of a DNA-binding activity to the R2 element of the enhancer (R. Ge, A. Kralli, R. Weinmann, and R. P. Ricciardi, J. Virol. 66:6969-6978, 1992). Employing transient transfection assays, we now provide direct evidence that the R2 element can confer repression in Ad12- but not Ad5-transformed cells. Repression by R2 was observed only in the presence of the positive enhancer element R1 and was dependent on (i) the number of the R2 elements and (ii) the relative arrangement of R2 and R1 elements. The putative R2-binding repressor protein, R2BF, was similar in molecular weight and binding specificity to members of the thyroid hormone/retinoic acid (RA) receptor family. RA treatment abrogated the R2-mediated repression in Ad12-transformed cells and had no effect on the activity of R2/R1-containing promoters in Ad5-transformed cells. These results are consistent with the presence of an R2-binding repressor in Ad12-transformed cells. In the absence of RA, the repressor compromises enhancer activity by interfering with the activity of the positive cis element R1. RA treatment of Ad12-transformed cells may render the repressor inactive.