gebhart/patrepeval huggingface dataset:
Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA) (2010)

For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists of a novel, foil-based centrifugal microfluidic cartridge including prestored liquid and dry reagents, and a commercially available centrifugal analyzer for incubation at 37 degrees C and real-time fluorescence detection. The system was characterized with an assay for the detection of the antibiotic resistance gene mecA of Staphylococcus aureus. The limit of detection was <10 copies and time-to-result was <20 min. Microfluidic unit operations comprise storage and release of liquid reagents, reconstitution of lyophilized reagents, aliquoting the sample into < or = 30 independent reaction cavities, and mixing of reagents with the DNA samples. The foil-based cartridge was produced by blow-molding and sealed with a self-adhesive tape. The demonstrated system excels existing PCR based lab-on-a-chip platforms in terms of energy efficiency and time-to-result. Applications are suggested in the field of mobile point-of-care analysis, B-detection, or in combination with continuous monitoring systems.
Fast multiresolution image operations in the wavelet domain (2003)

A wide class of operations on images can be performed directly in the wavelet domain by operating on coefficients of the wavelet transforms of the images and other matrices defined by these operations. Operating in the wavelet domain enables one to perform these operations progressively in a coarse-to-fine fashion, operate on different resolutions, manipulate features at different scales, trade off accuracy for speed, and localize the operation in both the spatial and the frequency domains. Performing such operations in the wavelet domain and then reconstructing the result is also often more efficient than performing the same operation in the standard direct fashion. In this paper, we demonstrate the applicability and advantages of this framework to three common types of image operations: image blending, 3D warping of images and sequences, and convolution of images and image sequences.
A comparative study of BSF layers for GaAs-based single-junction or multijunction concentrator solar cells (2006)

An effective back surface field is a key structural element for a high-efficiency GaAs concentrator solar cell, either in a multijunction or in a single-junction device. In this paper, several BSF materials are analysed, namely: (1) p++GaAs(Zn), (2) p+Ga0.5In0.5P(Zn) and (3) p++Al0.2Ga0.8As(C). The results of the comparison demonstrate that the best option is C-doped Al0.2Ga0.8As, which exhibits a low series resistance and behaves as an excellent minority carrier mirror; p++GaAs(Zn) shows reduced minority carrier mirror properties resulting from Zn diffusion and p+Ga0.5In0.5P(Zn) is shown to produce important series resistance problems because of an unfavourable heterojunction with GaAs.
CD45 Deficiency Drives Amyloid-β Peptide Oligomers and Neuronal Loss in Alzheimer's Disease Mice (2011)

Converging lines of evidence indicate dysregulation of the key immunoregulatory molecule CD45 (also known as leukocyte common antigen) in Alzheimer's disease (AD). We report that transgenic mice overproducing amyloid-β peptide (Aβ) but deficient in CD45 (PSAPP/CD45(-/-) mice) faithfully recapitulate AD neuropathology. Specifically, we find increased abundance of cerebral intracellular and extracellular soluble oligomeric and insoluble Aβ, decreased plasma soluble Aβ, increased abundance of microglial neurotoxic cytokines tumor necrosis factor-α and interleukin-1β, and neuronal loss in PSAPP/CD45(-/-) mice compared with CD45-sufficient PSAPP littermates (bearing mutant human amyloid precursor protein and mutant human presenilin-1 transgenes). After CD45 ablation, in vitro and in vivo studies demonstrate an anti-Aβ phagocytic but proinflammatory microglial phenotype. This form of microglial activation occurs with elevated Aβ oligomers and neural injury and loss as determined by decreased ratio of anti-apoptotic Bcl-xL to proapoptotic Bax, increased activated caspase-3, mitochondrial dysfunction, and loss of cortical neurons in PSAPP/CD45(-/-) mice. These data show that deficiency in CD45 activity leads to brain accumulation of neurotoxic Aβ oligomers and validate CD45-mediated microglial clearance of oligomeric Aβ as a novel AD therapeutic target.
Atomic Layer Deposition of SiO2 Films on BN Particles Using Sequential Surface Reactions (2000)

Alternating SiCl4 and H2O exposures were used to deposit SiO2 films with atomic layer control on BN particles. The high surface area of the BN particles facilitated the use of transmission Fourier transform infrared (FTIR) spectroscopy to monitor the sequential surface reactions. The BN particles initially displayed vibrational modes consistent with BOH* and BNH2* surface species. SiCl4 exposure at 700 K converted these species to SiClx* surface species. The subsequent H2O exposure at 700 K converted the SiClx* species to SiOH* surface species. Alternate exposures of SiCl4 and H2O yielded SiClx* and SiOH* species, respectively, sequentially depositing silicon and oxygen with atomic layer control. By repeating the sequential surface reactions, the absorbance of SiO2 bulk vibrational modes on the BN particles increased versus the number of SiCl4 and H2O reaction cycles. Transmission electron microscopy studies revealed fairly uniform SiO2 films of ∼28−38 Å on the edge planes of the BN particles after 32 reaction cycles at 700 K. SiO2 films on the basal planes of the BN particles were thinner and occurred in patches. X-ray photoelectron spectroscopy analysis was consistent with some uncoated regions on the BN particles. These ultrathin SiO2 films on BN particles may be useful to enhance the loading of BN particles in composite materials.
Differential Effects of CD28 Engagement and IL-12 on T Cell Activation by Altered Peptide Ligands (1998)

To futher our understanding of the mechanisms underlying the diverse effects of altered peptide ligands (APL) on T cell activation, we used a population of nonactivated spleen cells from mice that expressed a transgenic TCR specific for myelin basic protein Ac1-11 and peptide analogues that display either enhanced or decreased affinities for TCR/MHC to address the question whether APL-induced signaling through the TCR can regulate the capability of APC to activate T cells. We demonstrate that weak agonists APL are poor inducers of all aspects of the activation of both the responder T cells and the APC. Enhancement of the antigenic signal by augmenting the binding of the weak agonists to MHC reversed their defective activating capacity. Enhancement of costimulation by engagement of CD28 only resulted in augmentation of the capacity of the weak agonist APL to induce proliferation and IL-2/IL-3 production, but not CD40L or IL-12Rbeta2 chain expression on T cells, CD80/CD86 expression on APC, IL-12 secretion, or IFN-gamma production. Exogenous IL-12 promoted IFN-gamma production in the presence of the weak agonists. These studies demonstrate that there is a critical threshold of antigenic signal required for full activation of the T cell-APC interactions needed for the differentiation of Th1 cells. The provision of excess costimulation can overcome some of the defects in T cell activation by weak agonists, but is insufficient to induce a sufficient level of CD40L expression needed for engagement of CD40 on APC with subsequent IL-12 production and induction of IL-12Rbeta2 chain expression.
Roller-Bit Penetration Rate Response as a Function of Rock Properties and Well Depth (1986)

ABSTRACT A rate of penetration (ROP) prediction equation for an insert roller cone bit is determined from laboratory drilling tests as. a function of bit weight, well depth and laboratory measured rock properties. A complete description of each of the seven rock types used in the study is presented. A comparison of ROP at different flow rates for each rock shows minimal hydraulic cleaning problems at the base hydraulic energy levels chosen. The predictive equation will be valuable in predicting drilling rate and understanding how the rock properties affect it.
Synthesis and Biological Evaluation of Quinoline Salicylic Acids As P-Selectin Antagonists (2006)

Leukocyte recruitment of sites of inflammation and tissue injury involves leukocyte rolling along the endothelial wall, followed by firm adherence of the leukocyte, and finally transmigration of the leukocyte across cell junctions into the underlying tissue. The initial rolling step is mediated by the interaction of leukocyte glycoproteins containing active moieties such as sialyl Lewisx (sLex) with P-selectin expressed on endothelial cells. Consequently, inhibition of this interaction by means of a small molecule P-selectin antagonist is an attractive strategy for the treatment of inflammatory diseases such as arthritis. High-throughput screening of the Wyeth chemical library identified the quinoline salicylic acid class of compounds (1) as antagonists of P-selectin, with potency in in vitro and cell-based assays far superior to that of sLex. Through iterative medicinal chemistry, we identified analogues with improved P-selectin activity, decreased inhibition of dihydrooratate dehydrogenase, and acceptable CYP profiles. Lead compound 36 was efficacious in the rat AIA model of rheumatoid arthritis.
Crystal Structures of mPGES-1 Inhibitor Complexes Form a Basis for the Rational Design of Potent Analgesic and Anti-Inflammatory Therapeutics (2015)

Microsomal prostaglandin E synthase 1 (mPGES-1) is an α-helical homotrimeric integral membrane inducible enzyme that catalyzes the formation of prostaglandin E2 (PGE2) from prostaglandin H2 (PGH2). Inhibition of mPGES-1 has been proposed as a therapeutic strategy for the treatment of pain, inflammation, and some cancers. Interest in mPGES-1 inhibition can, in part, be attributed to the potential circumvention of cardiovascular risks associated with anti-inflammatory cyclooxygenase 2 inhibitors (coxibs) by targeting the prostaglandin pathway downstream of PGH2 synthesis and avoiding suppression of antithrombotic prostacyclin production. We determined the crystal structure of mPGES-1 bound to four potent inhibitors in order to understand their structure-activity relationships and provide a framework for the rational design of improved molecules. In addition, we developed a light-scattering-based thermal stability assay to identify molecules for crystallographic studies.
Lung Remodeling in Pulmonary Tuberculosis (2005)

Tuberculosis is a global public health catastrophe responsible for >8 million cases of illness and 2 million deaths annually. Pulmonary cavitation with cough-generated aerosol is the principle means of spread, and lung remodeling (healed cavitation, fibrosis, and bronchiectasis) is a major cause of lung disability, surpassing all other diffuse parenchymal lung diseases combined. Efficient granuloma turnover is mycobactericidal, and extracellular matrix is disbanded without scarring. In many with progressive disease, however, there is dysregulated granuloma turnover, liquefactive necrosis, and pathological scarring. The pathological mechanisms and the related immunological pathways underpinning these phenomena are reviewed in the present article. Further studies are needed to identify and develop specific immunotherapeutic interventions that target immunopathology, since they have the potential to substantially reduce spread.
Complications of Laparoscopic Colorectal Surgery (2004)

Procedures performed laparoscopically offer a wide variety of advantages to the patient, including less pain, quicker recovery, shorter duration of ileus, quicker return to full function, and a decreased immunological insult. Unfortunately, a laparoscopic procedure does not isolate the patient from a series of potential complications common to laparoscopic or any other type of surgery. Laparoscopic colon surgery is among the most difficult operations that can be performed laparoscopically and requires advanced laparoscopic skills. Complications resulting from laparoscopic colon surgery may be inherent to the procedure itself or to the laparoscopic technique.
In vitro validation of an ultra‐sensitive scanning fluorescence microscope for analysis of Circulating Tumor Cells (2013)

Analysis of circulating tumor cells ( CTC ) holds promise of providing liquid biopsies from patients with cancer. However, current methods include enrichment procedures. We present a method (CytoTrack ® ), where CTC from 7.5 mL of blood is stained, analyzed and counted by a scanning fluorescence microscope. The method was validated by breast cancer cells ( MCF ‐7) spiked in blood from healthy donors. The number of cells spiked in each blood sample was exactly determined by cell sorter and performed in three series of three samples spiked with 10, 33 or 100 cells in addition with three control samples for each series. The recovery rate of 10, 33 and 100 tumor cells in a blood sample was 55%, 70% and 78%, percent coefficient of variation ( CV %) for samples was 59%, 32% and 18%, respectively. None of the control samples contained CTC . In conclusion, the method has been validated to highly sensitively detect breast cancer cells in spiking experiments and should be tested on blood samples from breast cancer patients. The method could benefit from automation that could reduce the CV %, and further optimization of the procedure to increase the recovery.
Effect of Borna Disease Virus Infection on Athymic Rats (1985)

Homozygous athymic nude rats (rnu/rnu) infected intracerebrally with Borna disease virus produced relatively high titres of infectious virus in the central nervous system. However, no clinical signs of disease or pathological alterations could be found during a 100 day observation period. In contrast, heterozygous euthymic albino littermates (rnu/+), which were used as controls, reacted in a similar manner to immunocompetent Lewis rats. They developed behavioural alterations which coincided with encephalitis and retinitis. The results obtained confirm our previous concept that the genesis of Borna disease, at least in rats, is attributed to a cellular immune response.
In-vitro characterisation of the phagocytosis and fate of anthrax spores in macrophages and the effects of anti-PA antibody (2002)

Antibodies (Abs) to the protective antigen (PA) component of the anthrax toxins have anti-spore as well as anti-toxin activities. Anti-PA antisera and purified anti-PA Abs enhance the phagocytosis by murine-derived macrophages (MQs) of spores of the Ames and Sterne strains and retard the germination of extracellular spores in vitro. The fate after phagocytosis of untreated and anti-PA-treated spores was further studied in culture medium that supported phagocytosis without stimulating spore germination (Dulbecco's minimal essential medium with horse serum 10%). The spores germinated within cells of primary peritoneal murine MQs (C3H/HeN) and MQs of the RAW264.7 MQ-like cell line; germination was associated with a rapid decline in spore viability. Exposure of MQs to inhibitors of phago-endosomal acidification (bafilomycin A and chloroquine) reduced the efficiency of MQ killing and allowed outgrowth and replication of the organisms. Treatment of spores with anti-PA Abs stimulated their phagocytosis and was associated with enhanced MQ killing of the spores. The enhanced killing of spores correlated with the greater extent of germination of anti-PA-treated spores after phagocytosis. A PA null mutant of the Ames strain exhibited none of the effects associated with anti-PA Ab treatment ofthe parental strain. Thus, the anti-PA Ab-specific immunity induced by vaccines has anti-spore activities and its role in impeding the early stages of infection with Bacillusanthracis needs to be assessed.
Virus-neutralizing Antibodies to Hepatitis B Virus: The Nature of an Immunogenic Epitope on the S Gene Peptide (1986)

Using a murine monoclonal antibody (RF-HBs-1) which has been shown to be capable of neutralizing both ad and ay subtypes of hepatitis B virus (HBV), we have devised a competitive inhibition assay to measure the presence of virus-neutralizing antibodies in the sera of patients who have recovered from acute type B hepatitis. The majority of patients have this antibody in their serum. We also show that this antibody inhibits the binding of polymerized human serum albumin (pHSA) to the pHSA receptor site of the HBV particle, which has been proposed as an important site for the entry of HBV into liver cells. We have demonstrated that the epitope recognized by this antibody is dependent on the linkage of 24,000 and 28,000 mol. wt. polypeptides via a disulphide bond. This conformational determinant in the coat of the virus which is part of or near to the pHSA binding site is important in evoking a virus-neutralizing response.
High Frequency Behavior of Ceramic Multilayer Capacitors (1987)

The impedance of ceramic capacitors, made from different dielectric materials, was measured as a function of frequency from 1 MHz to 1 GHz. Most of the capacitors were of 0805 size and either end terminated or side terminated. The capacitance values ranged from a minimum of 50 pF to 100 nF. The high-frequency impedance measurements demonstrate that the inductance is a factor of two larger in end-terminated than in side-terminated ceramic muitilayer capacitors of size 0805. The inductance measured by two independent methods is 0.7 nH for the side-terminated 0805 capacitors. The inductance is only dependent on the geometry of the capacitor and independent of the dielectric material used. The capacitance and, in turn, the dielectric constant, remains constant for Z5U and X7R materials up to 100 MHz. For NPO materials the dielectric constant remains constant up to 1 GHz.
The effects of cyclophosphamide and irradiation singly and in combination upon SaI growth in A/J mice. (1987)

The effects of various doses of cyclophosphamide and low-dose (15 rads) radiation upon the size of tumors caused by 10(4) Sarcoma I (SaI) cells was determined. In intact A/Jax (A/J) recipients, the effect of the two agents singly and in combination was found to be dependent especially upon the dosage of cyclophosphamide and the time of its administration in relation to tumor inoculation. In cell transfer experiments to adult thymectomized, lethally irradiated, bone-marrow-restored (ATxXBM) mice, the effects of cyclophosphamide and irradiation appeared to be either overlapping (low dosages of cyclophosphamide) or additive (dosages of cyclophosphamide greater than or equal to 50 mg/kg), suggesting that the two agents exert their influence in dissimilar fashion, perhaps by injuring different cell types with the same basic function. The most pronounced conjoint effects are seen when low dosages of cyclophosphamide are given 3 days after the adoptive transfer of spleen cells from mice pretreated with low-dose irradiation. The implications of this observation with respect to immunotherapy are discussed.
Central Nervous System Effects of HMG CoA Reductase Inhibitors: Lovastatin and Pravastatin on Sleep and Cognitive Performance in Patients with Hypercholesterolemia (1994)

Sleep disturbances and decrements of daytime performance have been attributed to HMG-CoA reductase inhibitors. As a rule, lipophilic compounds more readily cross the blood-brain barrier and are more likely to affect central nervous system function. The authors compared the effects of lovastatin (40 mg), a lipophilic compound, to pravastatin (40 mg), a hydrophilic compound, in a 6-week, double-blind, randomized, placebo-controlled, three-way Latin square design, cross-over study on 22 men with hypercholesterolemia. Patients had LDL cholesterol of more than 165 mg/dL and triglyceride of less than 350 mg/dL after 6 weeks of a low-fat (< 30%), low-cholesterol (< 300 mg/day) diet. Compared with placebo, there were no significant effects of lovastatin or pravastatin on the following subjective and polysomnographic sleep measures: changes in total sleep time, time in each sleep stage, sleep efficiency, sleep latency, REM density, REM activity, and number of arousals. Similarly, there were no effects of the two drugs on measures of cognitive performance. A significant increase in the duration of nocturnal tumescence (NPT) was observed after 2 weeks of treatment with both study drugs. This effect was not significant after 6 weeks of treatment. Both lovastatin and pravastatin caused significant (P < .05 compared with placebo) decreases in total cholesterol (by 20.9 and 20.6%, respectively), LDL cholesterol (by 27.8 and 29.9%), and triglycerides (by 13.6 and 3.7%). Subjects' HDL increased by 2.3% with lovastatin (NS) and by 3.1% with pravastatin (P < .05). Lipoprotein(a) increased by 20.5% with lovastatin and by 1.1% with pravastatin; these changes were not significantly different from placebo.(ABSTRACT TRUNCATED AT 250 WORDS)
Few-Shot Adversarial Learning of Realistic Neural Talking Head Models (2019)

Several recent works have shown how highly realistic human head images can be obtained by training convolutional neural networks to generate them. In order to create a personalized talking head model, these works require training on a large dataset of images of a single person. However, in many practical scenarios, such personalized talking head models need to be learned from a few image views of a person, potentially even a single image. Here, we present a system with such few-shot capability. It performs lengthy meta-learning on a large dataset of videos, and after that is able to frame few- and one-shot learning of neural talking head models of previously unseen people as adversarial training problems with high capacity generators and discriminators. Crucially, the system is able to initialize the parameters of both the generator and the discriminator in a person-specific way, so that training can be based on just a few images and done quickly, despite the need to tune tens of millions of parameters. We show that such an approach is able to learn highly realistic and personalized talking head models of new people and even portrait paintings.
Electrolyte composition of pulmonary alveolar subphase in anesthetized rabbits (1986)

We measured the concentration of Na+, K+, Ca2+, and Cl- in the aqueous subphase of the alveolar lining by puncturing the most superficial alveoli of the exposed lungs of anesthetized rabbits with ion-selective microelectrodes and a nonselective KCl microelectrode. A buffered electrolyte solution bathed the lung surface to keep it moist and warm (38 +/- 1 degrees C) and to serve as a reference for each measurement of ionic concentration. The serum and alveolar concentrations (meq/l) were Na+ 134 +/- 6 and 135 +/- 5, K+ 3.4 +/- 0.2 and 7.3 +/- 0.7, Ca2+ 3.1 +/- 0.2 and 3.2 +/- 0.4, and Cl- 106 +/- 7 and 103 +/- 5 (mean +/- SD). Only K+ was significantly different (P less than 0.001). There was a small electrical potential difference between the alveolar lumen and the pleural surface (-3.5 +/- 0.8 mV, lumen negative) that was significantly different from zero (P less than 0.001). Although it is not possible to measure ion fluxes with these techniques, the results are consistent with active transport of one or more of the ions studied.