gebhart/patrepeval huggingface dataset:
Optical link design and validation testing of the Optical Payload for Lasercomm Science (OPALS) system (2014)

The Optical Payload for Lasercomm Science (OPALS) system developed by the Jet Propulsion Laboratory, California Institute of Technology, will be used for optical telecommunications link experiments from the International Space Station (ISS) to a ground telescope located at Table Mountain, CA. The launch of the flight terminal is scheduled for late February 2014 with an initially planned 90-day operations period following deployment on the exterior of the ISS. The simple, low-cost OPALS system will downlink a pre-encoded video file at 50 Mb/s on a 1550 nm laser carrier using on-off key (OOK) modulation and Reed-Solomon forward error correction. A continuous wave (cw) 976 nm multibeam laser beacon transmitted from the ground to the ISS will initiate link acquisition and tracking by the flight subsystem. Link analysis along with pre-flight results of the end-to-end free-space testing of the OPALS link are presented.
Cryptographic identification methods for smart cards in the process of standardization (1991)

Identification using integrated-circuit cards in credit card format with a built-in microcontroller (smart cards) is examined. The process by which the terminal's card adapter device verifies the authenticity of the smart card, called cryptographic node authentication, is examined. The methods used for node authentication, all of which are based on interrogation protocols, can be divided into the following categories on the basis of their fundamental cryptographic principles: challenge response with symmetrical key algorithms; challenge response with trapdoor public key algorithms; and zero-knowledge-based protocols. The various methods are described and compared, and the outlook for standardization is assessed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Nonviral Gene Delivery to Neural Stem Cells with Minicircles by Microporation (2013)

The main purpose of this work was to evaluate the transfection of novel DNA vectors, minicircles (mC), on embryonic stem cell-derived neural stem cells (NSC). We demonstrated that by combining microporation with mC, 75% of NSC expressing a transgene is achieved without compromising cell survival, morphology, and differentiation potential. When comparing mC with their plasmid DNA (pDNA) counterparts, both gave rise to similar transfection levels but cells harboring mC showed 10% higher cell viability, maintaining 90% of survival at least for 10 days. Long-term analysis showed that NSC harbor a higher number of mC copies and consequently exhibit higher transgene expression when compared to their pDNA counterpart. Taken together, our results offer the first insights on the use of mC as a novel and safe strategy to genetically engineer NSC envisaging their use as biopharmaceuticals in clinical settings for the treatment of neurodegenerative or neurological diseases.
Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries (1997)

We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transformed into Escherichia coli. Multiple rounds of enrichment with a target plasmid represented at 0.002-0.0001% resulted in over one-third positive clones. Our method will be useful for isolating even the rarest cDNAs starting from ESTs, isolated exons or homologous sequence information.
Conceptual Assessment of an Oblique Flying Wing Aircraft Including Control and Trim Characteristics (2008)

A method was developed to assist with the understanding of a unique configuration and investigate some of its stability and control attributes. Oblique wing aircraft concepts are a design option that is well understood, but has yet to be used in a production aircraft. Risk involved in choosing such a design can be averted through additional knowledge early in the concept evaluation phase. Analysis tools commonly used in early conceptual level analysis were evaluated for applicability to a non-standard aircraft design such as an oblique flying wing. Many tools used in early analyses make assumptions that are incompatible with the slewed wing configuration of the vehicle. Using a simplified set of tools, an investigation of a unique configuration was done as well as showing that the aircraft could be trimmed at given conditions. Wave drag was investigated to determine benefits for an oblique flying wing. This form of drag was reduced by the distribution of volume afforded by the slewing of the aircraft's wing. Once a reasonable concept was developed, aerodynamic conditions were investigated for static stability of the aircraft. Longitudinal and lateral trim were established simultaneously due to its asymmetric nature.
Small-Size LTE/WWAN Printed Loop Antenna With an Inductively Coupled Branch Strip for Bandwidth Enhancement in the Tablet Computer (2013)

The technique of using an inductively coupled branch strip for bandwidth enhancement of a simple printed loop antenna to achieve small size yet multiband operation to cover the LTE/WWAN bands (704-960 and 1710-2690 MHz) in the tablet computer is presented. The antenna's metal pattern occupies a small area of 10 × 34.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and is printed on a thin FR4 substrate. The branch strip is coupled to the loop antenna through a chip inductor. In the antenna's lower band, the branch strip can cause an additional resonance excited near a resonant mode contributed by the printed loop antenna and thereby greatly widens the antenna's low-band bandwidth to cover the 704-960 MHz band for the LTE700/GSM850/900 operations. While in the antenna's higher band, the chip inductor provides a high inductance and limits the excitation of the branch strip, thereby making the branch strip have negligible effects on the high-band operation. In this case, with the printed loop antenna excited in the higher band, the antenna can generate two higher order resonant modes to form a wide operating band for the GSM1800/1900/UMTS/LTE2300/2500 operations (1710-2690 MHz). Details of the proposed antenna are described, and the obtained results of the antenna are presented and discussed.
Composition Profiler: a tool for discovery and visualization of amino acid composition differences (2007)

As an exploratory data mining tool, our software can be used to guide feature selection for protein function or structure predictors. For classes of proteins with significant differences in frequencies of amino acids having particular physico-chemical (e.g. hydrophobicity or charge) or structural (e.g. alpha helix propensity) properties, Composition Profiler can be used as a rough, light-weight visual classifier.
Blind source camera identification (2005)

An interesting problem in digital forensics is that given a digital image, would it be possible to identify the camera model which was used to obtain the image. In this paper we look at a simplified version of this problem by trying to distinguish between images captured by a limited number of camera models. We propose a number of features which could be used by a classifier to identify the source camera of an image in a blind manner. We also provide experimental results and show reasonable accuracy in distinguishing images from the two and five different camera models using the proposed features.
Amine Scrubbing for CO 2 Capture (2009)

Amine scrubbing has been used to separate carbon dioxide (CO2) from natural gas and hydrogen since 1930. It is a robust technology and is ready to be tested and used on a larger scale for CO2 capture from coal-fired power plants. The minimum work requirement to separate CO2 from coal-fired flue gas and compress CO2 to 150 bar is 0.11 megawatt-hours per metric ton of CO2. Process and solvent improvements should reduce the energy consumption to 0.2 megawatt-hour per ton of CO2. Other advanced technologies will not provide energy-efficient or timely solutions to CO2 emission from conventional coal-fired power plants.
Red rubber bulb, cheap and effective vacuum drainage. (1989)

Red rubber bulbs have been used for vacuum drainage in head, neck, breast and several other operations by the authors since 1975 quite effectively without any major problems. The vacuum pressure of the red rubber bulbs was found to be higher than the expensive commercially available vacuum wound drainage device. The question of remaining old blood and infective microorganisms inside the reservoir for the reused ones were tested by the manual cleaning process and the standard sterile technique using steam under increased pressure (autoclave). The result is quite satisfactory. We encourage the use of this cheap and effective (made in Thailand) vacuum wound drainage in Thai hospitals and Thai medical schools.
Compounds Interacting with the Ethylene Receptor in Plants (2003)

Abstract: Some of the compounds binding to the ethylene receptor induce an ethylene response, but others prevent it. The compounds preventing an ethylene response have been developed into a means for protecting plants against ethylene and extending the life of some plant material. 1‐Methylcyclopropene (1‐MCP), a compound now commercially available under the names EthylBloc and SmartFresh™, is currently being used on flowers, fruit and vegetables with great success. In ethylene sensitive flowers, among other responses, it prevents senescence and abscission of plant organs; in fruit and vegetables it slows down the ripening process. Other similar compounds are now being developed for a range of methods of application.
Bureau of Mines - API Survey of Aviation Gasolines, 1969 (1970)

&lt;div class="htmlview paragraph"&gt;Inspection data are reported for 67 samples of three grades (80/87, 100/130, and 115/145) of commercial aviation gasolines produced in the United States during 1969. The results are compared with similar data for gasolines produced during 1964, the last year for which such data are available, and with recently revised specification requirements of the American Society for Testing and Materials.&lt;/div&gt;
HIV-1 p17 synthetic peptide vaccine HGP-30: induction of immune response in human subjects and preliminary evidence of protection against HIV challenge in SCID mice. (1995)

An HIV-1 p17 subunit vaccine, HGP-30, was evaluated in 38 HIV-1 seronegative individuals in phase I clinical trials in U.K. and U.S.A. The vaccine preparation induced cytotoxic T-cell (CTL) (11/25) and lymphocyte proliferation responses to KLH (19/20) and HGP-30/p17 (24/29) as well as antibody responses to HGP-30 (29/38) and KLH (38/38). The CTL activity was observed in a higher number of vaccine recipients (9/18) in the lower dose groups (10 and 25 micrograms/kg) than the vaccine recipients (2/7) in the 50 and 100 micrograms/kg dose group. These observations suggest that the 10-25 micrograms/kg vaccine dose may preferentially induce TH1 cell responses. TH1 cell responses have been suggested as important in inducing protective cell mediated immunity. The CTL response has been shown to be CD8+. In a pilot study in SCID mice, HIV-1 virus challenge studies in mice reconstituted with cells from an HGP-30 immunized individual showed protection against virus challenge as compared to SCID mice reconstituted with cells from a non-immunized subject. These studies suggest that HGP-30 is capable of inducing protective cellular immunity.
CD30 EXPRESSION IDENTIFIES A FUNCTIONAL ALLOREACTIVE HUMAN T-LYMPHOCYTE SUBSET1 (1998)

CD30+ T lymphocytes may constitute an important immunoregulatory subset in human allograft rejection.
Partial Purification and Properties of an Alkaline α-Galactosidase from Mature Leaves of Cucurbita pepo (1983)

A fourth molecular from of alpha-galactosidase, designated L(IV), an alkaline alpha-galactosidase, was isolated from leaves of Cucurbita pepo and purified 165-fold. It was active over a narrow pH range with optimal hydrolysis of p-nitrophenyl-alpha-d-galactoside and stachyose at pH 7.5. The rate of stachyose hydrolysis was 10 times that of raffinose. K(m) determinations in McIlvaine buffer (200 millimolar Na(2)-phosphate, 100 millimolar citric acid, pH 7.5) for p-nitrophenyl-alpha-d-galactoside, stachyose, and raffinose were 1.40, 4.5, and 36.4 millimolar, respectively. L(IV) was partially inhibited by Ca(2+), Mg(2+), and Mn(2+), more so by Ni(2+), Zn(2+), and Co(2+), and highly so by Cu(2+), Ag(2+), Hg(2+) and by p-chloromercuribenzoate. It was not inhibited by high concentrations of the substrate p-nitrophenyl-alpha-d-galactoside or by myo-inositol, but alpha-d-galactose was a strong inhibitor. As observed for most other forms of alpha-galactosidase, L(IV) only catalyzed the hydrolysis of glycosides possessing the alpha-d-galactose configuration at C(1), C(2), and C(4), and did not hydrolyze p-nitrophenyl-alpha-d-fucoside (alpha-d-galactose substituted at C(6)). The enzyme was highly sensitive to buffers and chelating agents. Maximum hydrolytic activity for p-nitrophenyl-alpha-d-galactoside was obtained in McIlvaine buffer (pH 7.5). In 10 millimolar triethanolaminehydrochloride-NaOH (pH 7.5) or 10 millimolar Hepes-NaOH (pH 7.5), hydrolytic activity was virtually eliminated, but the addition of low concentrations of either ethylenediaminetetraacetate or citrate to these buffers restored activity almost completely. Partial restoration of activity was also observed, but at higher concentrations, with pyruvate and malate. Similar effects were found for stachyose hydrolysis, but in addition some inhibition of L(IV) in McIlvaine buffer, possibly due to the high phosphate concentration, was observed with this substrate. It is questionable whether the organic acid anions possess any regulatory control of L(IV)in vivo. It was possible that the results reflected the ability of these anions, and ethylene-diaminetetraacetate, to restore L(IV) activity through coordination with some toxic cation introduced as a buffer contaminant.
The GTP binding proteins Gem and Rad are negative regulators of the Rho–Rho kinase pathway (2002)

The cytoskeletal changes that alter cellular morphogenesis and motility depend upon a complex interplay among molecules that regulate actin, myosin, and other cytoskeletal components. The Rho family of GTP binding proteins are important upstream mediators of cytoskeletal organization. Gem and Rad are members of another family of small GTP binding proteins (the Rad, Gem, and Kir family) for which biochemical functions have been mostly unknown. Here we show that Gem and Rad interface with the Rho pathway through association with the Rho effectors, Rho kinase (ROK) alpha and beta. Gem binds ROKbeta independently of RhoA in the ROKbeta coiled-coil region adjacent to the Rho binding domain. Expression of Gem inhibited ROKbeta-mediated phosphorylation of myosin light chain and myosin phosphatase, but not LIM kinase, suggesting that Gem acts by modifying the substrate specificity of ROKbeta. Gem or Rad expression led to cell flattening and neurite extension in N1E-115 neuroblastoma cells. In interference assays, Gem opposed ROKbeta- and Rad opposed ROKalpha-mediated cell rounding and neurite retraction. Gem did not oppose cell rounding initiated by ROKbeta containing a deletion of the Gem binding region, demonstrating that Gem binding to ROKbeta is required for the effects observed. In epithelial or fibroblastic cells, Gem or Rad expression resulted in stress fiber and focal adhesion disassembly. In addition, Gem reverted the anchorage-independent growth and invasiveness of Dbl-transformed fibroblasts. These results identify physiological roles for Gem and Rad in cytoskeletal regulation mediated by ROK.
The Mycobacterial Heparin-Binding Hemagglutinin Is a Protective Antigen in the Mouse Aerosol Challenge Model of Tuberculosis (2004)

The heparin-binding hemagglutinin (HBHA) of Mycobacterium tuberculosis is a surface-expressed adhesin that can affect binding to host cells via a unique, methylated, carboxyl-terminal, lysine-, alanine-, and proline-rich repeat region. It has been implicated in extrapulmonary dissemination of M. tuberculosis from the lung following the initial infection of the host. To assess the vaccine potential of this protein, purified preparations of HBHA were emulsified in a dimethyldioctadecylammonium bromide-monophosphoryl lipid A adjuvant and tested for the ability to reduce M. tuberculosis infection in the mouse aerosol challenge model for tuberculosis. The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge. Although a notable level of serum antibody to HBHA was elicited after three immunizations and the antibodies were able to bind to the surface of M. tuberculosis, passive immunization with monoclonal antibodies directed against HBHA did not protect in the challenge model. Compared to adjuvant controls, an elevated gamma interferon response was generated by splenic and lymph node-derived T cells from immunized mice in the presence of macrophages pulsed with purified HBHA or infected with live M. tuberculosis, suggesting that the effective immunity may be cell mediated. Efforts to construct effective recombinant HBHA vaccines in fast-growing Mycobacterium smegmatis have been unsuccessful so far, which indicates that distinctive posttranslational modifications present in the HBHA protein expressed by M. tuberculosis are critical for generating effective host immune responses. The vaccine studies described here demonstrate that HBHA is a promising new vaccine candidate for tuberculosis.
Comparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo (2018)

Small interfering RNA (siRNA)-based drugs require chemical modifications or formulation to promote stability, minimize innate immunity, and enable delivery to target tissues. Partially modified siRNAs (up to 70% of the nucleotides) provide significant stabilization in vitro and are commercially available; thus are commonly used to evaluate efficacy of bio-conjugates for in vivo delivery. In contrast, most clinically-advanced non-formulated compounds, using conjugation as a delivery strategy, are fully chemically modified (100% of nucleotides). Here, we compare partially and fully chemically modified siRNAs in conjugate mediated delivery. We show that fully modified siRNAs are retained at 100x greater levels in various tissues, independently of the nature of the conjugate or siRNA sequence, and support productive mRNA silencing. Thus, fully chemically stabilized siRNAs may provide a better platform to identify novel moieties (peptides, aptamers, small molecules) for targeted RNAi delivery.
Selective metallation of thiophene and thiazole rings with magnesium amide base (2001)

Selective hydrogen–metal exchange reaction of thiophene and thiazole proceeded smoothly with magnesium amide base (iPr2NMgCl) under mild conditions.
pH‐responsive gelatin microspheres for oral delivery of anticancer drug methotrexate (1995)

Abstract Multiple unit delivery dosage forms of biodegradable gelatin microspheres containing the anticancer drug methotrexate (GMM) of various mean particle sizes (1–5, 5–10, and 15–20 μm) were prepared by the polymer dispersion technique and were crosslinked with glutaraldehyde. The GMM were coated with biodegradable natural polymers, namely alginate (AGMM) and chitosan (CGMM), which differ in their pH sensitivity, to obtain two different types of pH dependent delivery systems for oral delivery of methotrexate (MTX). The in vitro release profiles of MTX from AGMM and CGMM were determined in simulated gastric medium, intestinal medium, and in media simulating gastrointestinal tract conditions. The effect of the concentration of coating polymer and particle size on the release rate of MTX from both AGMM and CGMM were also studied. Both AGMM and CGMM provided controlled release of MTX following a zero‐order release pattern in gastric and intestinal fluids for prolonged periods of time. The release rate of MTX decreased with an increase in concentration of the coating polymer as well as an increase in particle size of the microspheres. Both AGMM and CGMM showed good potential as pH dependent multiple unit delivery systems for the controlled release of MTX in oral administration. © 1995 John Wiley &amp; Sons, Inc.
RiftNet: Radio Frequency Classification for Large Populations (2021)

Radio transmitters can be identified by their unique radio frequency (RF) fingerprints. Hardware differences and manufacturing variations between different RF devices impart the unique RF fingerprints to their transmitted waveforms, which can be exploited to accurately distinguish them. Most previous work has focused on using expert-defined physics-based features to distinguish between 100 or less individual devices, with more recent trends turning to machine learning, and deep learning in particular. In this paper, we present a deep learning approach that scales to 10,000 devices, across different signal types, with upwards of 95% accuracy, which we call RiftNet (Resampling in Frequency and Time Network). RiftNet is particularly suited to pulling out generic signal features that may be relevant to other RF machine learning tasks, beyond just fingerprinting,
Demonstration of the heterostructure field-effect transistor as an optical modulator (1991)

A new semiconductor waveguide absorption modulator is demonstrated utilizing the heterostructure field-effect transistor structure. The modulator of 300 μm length and 10 μm width achieves an extinction ratio of 8 for a gate voltage change of 2.5 V and an absorption change greater than 2300 cm−1. The transistor transconductance is 92 ms/mm for a 1 μm device and an identical structure has been reported as an edge-emitting laser providing an ideal combination for optoeletronic integration.
COSMOLOGICAL EVOLUTION OF MODIFIED CHAPLYGIN GAS INTERACTING WITH BAROTROPIC BACKGROUND FLUID (2009)

Based on the observation that the equation of state parameter ω can cross the phantom dividing ω = -1 and has a 99% probability of being ω &lt; -1, we introduce an interaction between the modified Chaplygin gas and the barotropic background fluid. By studying the dynamical evolution of the MCG model with interaction and the statefinder diagnostic in autonomous system, we find that the MCG vacuum-energy-dominated phase is the only stable attractor, the universe evolves from the matter-dominated phase to the MCG vacuum-energy-dominated phase, and the effect of the transfer strength parameter c is evident. In addition, we have plotted the evolution trajectories of the interacting CG, GCG and MCG models with barotropic background fluid in the statefinder parameter (s - r)-plane to illustrate the discriminations among these scenarios.
Palladium-Catalyzed Stereocontrolled Vinylation of Azoles and Phenothiazine (2002)

[reaction: see text] Vinylation of various azoles (pyrrole, indole, carbazole, and their derivatives) and phenothiazine with vinyl bromides catalyzed by palladium-phosphine complexes results in the respective N-vinylazoles in 30-99% yields. This reaction with cis- and trans-beta-bromostyrenes is stereospecific giving the respective products with full retention of configuration.