gebhart/patrepeval huggingface dataset:
Lipoxin A4 modulates transmigration of human neutrophils across intestinal epithelial monolayers. (1993)

Neutrophil (PMN) migration across intestinal epithelial barriers, such as occurs in many disease states, results in modifications in epithelial barrier. Here, we investigated the impact of lipoxin A4 (LXA4), an eicosanoid with counterregulatory inflammatory roles, on PMN migration across cultured monolayers of the human intestinal epithelial cell line T84. Transepithelial migration of PMN was assessed in the apical-to-basolateral direction and in the basolateral-to-apical direction. In the apical-to-basolateral direction, preexposure of PMN to LXA4 (10 nM, 15 min) stimulated an 87 +/- 5% increase in transepithelial migration of PMN as determined by a PMN myeloperoxidase assay. The LXA4-elicited effect on transmigration was present throughout the 2-h assay period and was not secondary to LXA4 effects on epithelial monolayer integrity as judged by measurement of transepithelial resistance. PMN migration in the basolateral-to-apical direction was modulated by LXA4 with a comparable time- and concentration-dependence to that in the apical-to-basolateral direction. However, qualitative differences in how LXA4 modulates transmigration in the two opposing directions were observed. In the basolateral-to-apical direction, preexposure of PMN to LXA4 (10 nM, 15 min) diminished PMN transepithelial migration by 33 +/- 4%. Structure-function studies revealed that LXA4 and 11-trans-LXA4 (50% of LXA4 effect), but not LXB4, inhibited basolateral-to-apical PMN transmigration. The action of LXA4 was not sensitive to inhibitors of cyclooxygenase or specific leukotriene biosynthesis, but was sensitive to staurosporine, a protein kinase C inhibitor. These results suggest that migration of PMN across epithelia in the physiological direction may be qualitatively different following PMN exposure to eicosanoids. We propose that such retention of PMN at this specific anatomic location may serve an important role in mucosal defense.
Pluramycins. Old Drugs Having Modern Friends in Structural Biology (1996)

ADVERTISEMENT RETURN TO ISSUEPREVArticlePluramycins. Old Drugs Having Modern Friends in Structural BiologyMark R. Hansen and Laurence H. HurleyView Author Information Drug Dynamics Institute, Division of Medicinal Chemistry, College of Pharmacy, The University of Texas at Austin, Austin, Texas 78712Cite this: Acc. Chem. Res. 1996, 29, 5, 249–258Publication Date (Web):May 14, 1996Publication History Received25 September 1995Published online14 May 1996Published inissue 14 May 1996https://pubs.acs.org/doi/10.1021/ar950167ahttps://doi.org/10.1021/ar950167aresearch-articleACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views798Altmetric-Citations130LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Genetics,Guanine,Nucleic acid structure,Reactivity,Selectivity Get e-Alerts
Attenuation of antigen-induced airway hyperresponsiveness in CGRP-deficient mice (2002)

Bronchial hyperresponsiveness and eosinophilia are major characteristics of asthma. Calcitonin gene-related peptide (CGRP) is a neuropeptide that has various biological actions. In the present study, we questioned whether CGRP might have pathophysiological roles in airway hyperresponsiveness and eosinophilia in asthma. To determine the exact roles of endogenous CGRP in vivo, we chose to study antigen-induced airway responses using CGRP gene-disrupted mice. After ovalbumin sensitization and antigen challenge, we assessed airway responsiveness and measured proinflammatory mediators. In the sensitized CGRP gene-disrupted mice, antigen-induced bronchial hyperresponsiveness was significantly attenuated compared with the sensitized wild-type mice. Antigen challenge induced eosinophil infiltration in bronchoalveolar lavage fluid, whereas no differences were observed between the wild-type and CGRP-mutant mice. Antigen-induced increases in cysteinyl leukotriene production in the lung were significantly reduced in the CGRP-disrupted mice. These findings suggest that CGRP could be involved in the antigen-induced airway hyperresponsiveness, but not eosinophil infiltration, in mice. The CGRP-mutant mice may provide appropriate models to study molecular mechanisms underlying CGRP-related diseases.
A transimpedance amplifier for capacitive micromachined ultrasonic transducers (2015)

In this thesis a design of a CMOS transimpedance amplifier (TIA) for a capacitive micro-machined ultrasonic transducer (CMUT) is presented.CMUT's have a high electrical impedance when used as receivers.Any capacitance between the CMUT and a high impedance amplifier will degrade the frequency response.So, we need to amplify the current rather than the voltage.This approach requires a transimpedance amplifier.The designed TIA has a 30 MHz bandwidth and 400 k transimpedance gain.The total input referred current noise of the TIA is 270 fA/ Hz at 10 MHz.The noise figure of the TIA is 2.7 dB at 10 MHz when connected to the CMUT with 200 k source resistance.The power consumption of the TIA is 10.5 mW and the size of the TIA layout is 133m x 45m.The TIA chip will be fabricated in AMS C35B4C3 (0.35m) process.
Functional characterization of an EGF receptor with a truncated extracellular domain expressed in glioblastomas with EGFR gene amplification. (1994)

The most common type of alteration of the epidermal growth factor receptor gene (EGFR) in human glioblastomas results in the synthesis of an aberrant mRNA lacking 801 bases that encode amino acids 6-273 of the receptor's extracellular domain. To study the effects of this mutation on receptor function, we have developed chinese hamster ovary cell transfectants which express the mutant EGF receptor. Comparison of wild-type and mutant receptor properties in this cell host indicates that the truncated receptor does not bind EGF or TGF-alpha and, consequently, DNA synthesis is not stimulated in cultures of mutant transfectants by either grown factor. However, levels of DNA synthesis determined for mutant transfectants in serum-free media are several-fold higher than those determined for corresponding cultures of wild-type transfectants. Western blot analysis with anti-phosphotyrosine antibody indicates that the mutant receptor is constitutively phosphorylated in CHO cells, and the same analysis applied to lysates of glioblastoma biopsies reveals the altered receptor is readily detectable as a phosphotyrosine protein in tumors for which there is evidence of corresponding EGFR gene and transcript alterations. In total, these results indicate that the aberrant EGF receptor synthesized in glioblastomas, and which lacks a portion of the extracellular domain necessary for ligand binding, is an activated tyrosine kinase.
Chronic β2-adrenergic receptor stimulation increases proliferation of human cardiac fibroblasts via an autocrine mechanism (2003)

Chronic beta(2)-AR stimulation increases proliferation of human cardiac fibroblasts via a mechanism involving increased secretion of heat-sensitive growth factors.
Nucleolin: A Multifunctional Major Nucleolar Phosphoprotein (1998)

Nucleolin is a major protein of exponentially growing eukaryotic cells where it is present in abundance at the heart of the nucleolus. It is highly conserved during evolution. Nucleolin contains a specific bipartite nuclear localization signal sequence and possesses a number of unusual structural features. It has unique tripartite structure and each domain performs a specific function by interacting with DNA or RNA or proteins. Nucleolin exhibits intrinsic self-cleaving, DNA helicase, RNA helicase and DNA-dependent ATPase activities. Nucleolin also acts as a sequence-specific RNA binding protein, an autoantigen, and as the component of a B cell specific transcription factor. Its phosphorylation by cdc2, CK2, and PKC-zeta modulate some of its activities. This multifunctional protein has been implicated to be involved directly or indirectly in many metabolic processes such as ribosome biogenesis (which includes rDNA transcription, pre-rRNA synthesis, rRNA processing, ribosomal assembly and maturation), cytokinesis, nucleogenesis, cell proliferation and growth, cytoplasmic-nucleolar transport of ribosomal components, transcriptional repression, replication, signal transduction, inducing chromatin decondensation and many more (see text). In plants it is developmentally, cell-cycle, and light regulated. The regulation of all these functions of a single protein seems to be a challenging puzzle.
Circular Logic: Nonribosomal Peptide-like Macrocyclization with a Ribosomal Peptide Catalyst (2010)

A protease from ribosomal peptide biosynthesis macrocyclizes diverse substrates, including those resembling nonribosomal peptide and hybrid polyketide-peptide products. The proposed mechanism is analogous to thioesterase-catalyzed chemistry, but the substrates are amide bonds rather than thioesters.
The Management and Mismanagement of Taxable Assets (2001)

Although the aggregate amount of taxable money under management actually exceeds the value of tax-exempt money such as in pensions and endowments, far too often these assets are invested without proper consideration for taxes. The authors identify sources of taxable asset mismanagement and give methods of improvement. They also describe considerations for selecting a tax-efficient manager and give details of actually effecting a tax-efficient strategy. Lastly, the authors close with a roster of actions a taxable investor should and should not do.
Improving data access in P2P systems (2002)

The authors present Gridella, a Gnutella-compatible P2P system. Gridella is based on the Peer-Grid (P-Grid) approach, which draws on research in distributed and cooperative information systems to provide a decentralized, scalable data access structure. Gridella improves the highly chaotic and inefficient Gnutella infrastructure with directed search and advanced concepts, thus enhancing efficiency and providing a model for further analysis and research.
Human SOD1 before Harboring the Catalytic Metal (2005)

SOD1 has to undergo several post-translational modifications before reaching its mature form. The protein requires insertion of zinc and copper atoms, followed by the formation of a conserved S-S bond between Cys-57 and Cys-146 (human numbering), which makes the protein fully active. In this report an NMR structural investigation of the reduced SH-SH form of thermostable E,Zn-as-SOD1 (E is empty; as is C6A, C111S) is reported, characterizing the protein just before the last step leading to the mature form. The structure is compared with that of the oxidized S-S form as well as with that of the yeast SOD1 complexed with its copper chaperone, CCS. Local conformational rearrangements upon disulfide bridge reduction are localized in the region near Cys-57 that is completely exposed to the solvent in the present structure, at variance with the oxidized forms. There is a local disorder around Cys-57 that may serve for protein-protein recognition and may possibly be involved in intermolecular S-S bonds in familial amyotrophic lateral sclerosis-related SOD1 mutants. The structure allows us to further discuss the copper loading mechanism in SOD1.
Designing immunoconjugates for cancer therapy (2012)

Conjugation chemistry and recombinant technologies have had a significant impact on the therapeutic prospects of immunoconjugates, particularly in hematopoietic diseases. Continued concerted efforts from different scientific disciplines are needed, together with newer treatment paradigms, for greater progress in the more challenging therapy of solid tumors.
A Characterization of Brain-Computer Interface Performance Trade-Offs Using Support Vector Machines and Deep Neural Networks to Decode Movement Intent (2018)

Laboratory demonstrations of brain-computer interface (BCI) systems show promise for reducing disability associated with paralysis by directly linking neural activity to the control of assistive devices. Surveys of potential users have revealed several key BCI performance criteria for clinical translation of such a system. Of these criteria, high accuracy, short response latencies, and multi-functionality are three key characteristics directly impacted by the neural decoding component of the BCI system, the algorithm that translates neural activity into control signals. Building a decoder that simultaneously addresses these three criteria is complicated because optimizing for one criterion may lead to undesirable changes in the other criteria. Unfortunately, there has been little work to date to quantify how decoder design simultaneously affects these performance characteristics. Here, we systematically explore the trade-off between accuracy, response latency, and multi-functionality for discrete movement classification using two different decoding strategies-a support vector machine (SVM) classifier which represents the current state-of-the-art for discrete movement classification in laboratory demonstrations and a proposed deep neural network (DNN) framework. We utilized historical intracortical recordings from a human tetraplegic study participant, who imagined performing several different hand and finger movements. For both decoders, we found that response time increases (i.e., slower reaction) and accuracy decreases as the number of functions increases. However, we also found that both the increase of response times and the decline in accuracy with additional functions is less for the DNN than the SVM. We also show that data preprocessing steps can affect the performance characteristics of the two decoders in drastically different ways. Finally, we evaluated the performance of our tetraplegic participant using the DNN decoder in real-time to control functional electrical stimulation (FES) of his paralyzed forearm. We compared his performance to that of able-bodied participants performing the same task, establishing a quantitative target for ideal BCI-FES performance on this task. Cumulatively, these results help quantify BCI decoder performance characteristics relevant to potential users and the complex interactions between them.
Variable dimension weighted universal vector quantization and noiseless coding (2002)

A new algorithm for variable dimension weighted universal coding is introduced. Combining the multi-codebook system of weighted universal vector quantization (WUVQ), the partitioning technique of variable dimension vector quantization, and the optimal design strategy common to both, variable dimension WUVQ allows mixture sources to be effectively carved into their component subsources, each of which can then be encoded with the codebook best matched to that source. Application of variable dimension WUVQ to a sequence of medical images provides up to 4.8 dB improvement in signal to quantization noise ratio over WUVQ and up to 11 dB improvement over a standard full-search vector quantizer followed by an entropy code. The optimal partitioning technique can likewise be applied with a collection of noiseless codes, as found in weighted universal noiseless coding (WUNC). The resulting algorithm for variable dimension WUNC is also described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cathepsin L deficiency as molecular defect of furless: hyperproliferation of keratinocytes and pertubation of hair follicle cycling (2000)

Lysosomal cysteine proteinases of the papain family are involved in lysosomal bulk proteolysis, major histocompatibility complex class II mediated antigen presentation, prohormone processing, and extracellular matrix remodeling. Cathepsin L (CTSL) is a ubiquitously expressed major representative of the papain-like family of cysteine proteinases. To investigate CTSL in vivo functions, the gene was inactivated by gene targeting in embryonic stem cells. CTSL-deficient mice develop periodic hair loss and epidermal hyperplasia, acanthosis, and hyperkeratosis. The hair loss is due to alterations of hair follicle morphogenesis and cycling, dilatation of hair follicle canals, and disturbed club hair formation. Hyperproliferation of hair follicle epithelial cells and basal epidermal keratinocytes-both of ectodermal origin-are the primary characteristics underlying the mutant phenotype. Pathological inflammatory responses have been excluded as a putative cause of the skin and hair disorder. The phenotype of CTSL-deficient mice is reminiscent of the spontaneous mouse mutant furless (fs). Analyses of the ctsl gene of fs mice revealed a G149R mutation inactivating the proteinase activity. CTSL is the first lysosomal proteinase shown to be essential for epidermal homeostasis and regular hair follicle morphogenesis and cycling.
A protocol-independent technique for eliminating redundant network traffic (2000)

We present a technique for identifying repetitive information transfers and use it to analyze the redundancy of network traffic. Our insight is that dynamic content, streaming media and other traffic that is not caught by today's Web caches is nonetheless likely to derive from similar information. We have therefore adapted similarity detection techniques to the problem of designing a system to eliminate redundant transfers. We identify repeated byte ranges between packets to avoid retransmitting the redundant data. We find a high level of redundancy and are able to detect repetition that Web proxy caches are not. In our traces, after Web proxy caching has been applied, an additional 39% of the original volume of Web traffic is found to be redundant. Moreover, because our technique makes no assumptions about HTTP protocol syntax or caching semantics, it provides immediate benefits for other types of content, such as streaming media, FTP traffic, news and mail.
Graft vascular disease after cardiac transplantation and its relationship to mean acute rejection score. (2005)

This study showed that the development of coronary artery fibrointimal hyperplasia, but not atherosclerosis, was significantly related to mean acute rejection score.
Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva (2003)

To evaluate alternative approaches to the serological diagnosis of dengue virus (DEN) infection, the detection of DEN-specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva specimens was assessed in 147 patients with symptoms of DEN infection seen at the Ministry of Health in Nicaragua. Seventy-two serum samples were determined to be positive for anti-DEN antibodies by IgM capture enzyme-linked immunosorbent assay, the routine diagnostic procedure. Serum and saliva specimens were obtained from 50 healthy adults as additional controls. IgM was detected in the saliva of 65 of the 72 serum IgM-positive cases, 6 of the 75 serum IgM-negative cases, and none of the control group, resulting in a sensitivity of 90.3% and a specificity of 92.0% and demonstrating that salivary IgM is a useful diagnostic marker for DEN infection. Detection of IgA in serum may be another feasible alternative for the diagnosis of DEN infection, with serum IgA found in 68 (94.4%) of the IgM-positive cases. In contrast, detection of IgA in saliva was not found to be a useful tool for DEN diagnosis in the present study. Further studies of the kinetics of antibody detection in another set of 151 paired acute- and convalescent-phase serum samples showed that DEN-specific IgA antibodies were detected in more acute-phase samples than were IgM antibodies. Thus, we conclude that DEN-specific IgA in serum is a potential diagnostic target. Furthermore, given that saliva is a readily obtainable, noninvasive specimen, detection of DEN-specific salivary IgM should be considered a useful, cheaper diagnostic modality with similar sensitivity and specificity to IgM detection in serum.
Improved Method for Rearing Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)1 (1988)

The described rearing method approximates the natural environment for larvae of Diabrotica virgifera virgifera LeConte more closely than do previous methods: eggs are in close proximity to corn roots when they hatch, neonate larvae are not handled, and larvae are reared in soil. In addition, the method is less labor-intensive. Our quality-control samples indicate a mean (±SD) return of 53.0% ±5.0 from eggs to adults and a mean return of 72.7% ± 10.2 from neonate larvae to adults. Adult females have a mean head-capsule width of 1.22 mm ± 0.4. Moreover, our new method for handling adults, both before and during mating, has resulted in a significant improvement in mating; percentage of mated females increased from a mean of 66 to a mean of 95%.
BDCA-2, BDCA-3, and BDCA-4: Three Markers for Distinct Subsets of Dendritic Cells in Human Peripheral Blood (2000)

We have generated a panel of mAbs that identify three presumably novel human dendritic cell Ags: BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c(+) CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c(+) CD11c(bright) CD123(dim), or on any other cells in blood. BDCA-4 is also expressed on monocyte-derived and CD34(+) cell-derived dendritic cells. Expression of all three Ags dramatically changes once blood dendritic cells undergo in vitro maturation. BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, expression of BDCA-3 is up-regulated on both plasmacytoid CD11c(-) CD123(bright) dendritic cells and CD1c(+) CD11c(bright) CD123(dim) dendritic cells, and expression of BDCA-4 is up-regulated on CD1c(+) CD11c(bright) CD123(dim) dendritic cells. BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling. The three presumably novel Ags serve as specific markers for the respective subpopulations of blood dendritic cells in fresh blood and will be of great value for their further analysis and to evaluate their therapeutic potential.
Vision-based car-following: detection, tracking, and identification (2003)

The authors have developed a vision system for automatic car following and object classification. The CARTRACK system can reliably detect, track, and identify the back of an automobile in a dynamic image taken from a following car in the same lane or in a neighbor lane. The detection and tracking system exploits the symmetry property of the rear view of normal cars. The class of objects that are detected by CARTRACK includes normal cars of all sizes as well as trucks and conventional trailers. Parallel to the tracking process, a model-based identification module identifies the type of vehicle being followed. For objects of known size, it also facilitates distance estimation. Deformable 2D models (planar feature graphs) constructed from various visual features are used. The image features in a region of interest selected by the symmetry-based detection process are matched with the model objects by means of an elastic net technique.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>