gebhart/patrepeval huggingface dataset:
Coded labels with amorphous magnetoelastic resonators (2002)

In this paper, a coding and decoding technique for labels based on magnetoelastic resonators is presented. For coding of the labels, the length of the resonators as well as their angular direction in the label is used. As an external bias magnetic field is used for decoding, no semihard magnetic strips are needed in the label. Low cost plastic housings with a low damping coating have been developed for these labels to achieve a high-level resonator signal.
Cross-linking of a monomeric 39/34-kDa dispase fragment of von Willebrand factor (Leu-480/Val-481-Gly-718) to the N-terminal region of the .alpha.-chain of membrane glycoprotein Ib on intact platelets with bis(sulfosuccinimidyl) suberate (1989)

A 39/34-kilodalton (kDa) monomeric dispase fragment of von Willebrand factor (vWF) has been purified by heparin affinity chromatography. Detailed structural analysis of the individual 39- and 34-kDa fragments indicated that they had identical amino acid sequences extending from Leu-480/Val-481 to Gly-718 with an intramolecular disulfide bond between Cys-509 and Cys-695. In addition to the binding site for heparin, the 39/34-kDa fragment also contained binding sites for collagen and for platelet membrane glycoprotein (GP) Ib. Unlike native vWF, the 39/34-kDa fragment bound to GP Ib without the requirement for a modulator but showed increased binding in the presence of botrocetin. The 39/34-kDa vWF fragment was cross-linked to intact human platelets by using the membrane-impermeable, homobifunctional cross-linking reagent bis(sulfosuccinimidyl) suberate. Two distinct cross-linked species of similar molecular weight (220/200 kDa, nonreduced; 190/175 kDa, reduced) were identified by SDS-polyacrylamide gel electrophoresis and autoradiography, consistent with the cross-linking of the 125I-labeled 39/34-kDa vWF fragment to GP Ib. The formation of these cross-linked species was enhanced 1.5-2.5-fold in the presence of the modulator botrocetin. The platelet membrane protein involved in cross-linking was shown unequivocally to be GP Ib since (i) neither cross-linked species was formed with Bernard-Soulier syndrome platelets, which genetically lack the GP Ib-IX complex, (ii) both cross-linked species were specifically immunoprecipitated by anti-GP Ib polyclonal and monoclonal antibodies, and (iii) the formation of the cross-linked species was completely inhibited only by those anti-GP Ib-IX complex monoclonal antibodies that inhibited vWF-GP Ib-IX complex interaction. Proteolysis of cross-linked platelets with endoproteinase Lys-C, which preferentially cleaves off the N-terminal peptide domain on the alpha-chain of GP Ib, indicated that the 39/34-kDa vWF fragment was cross-linked exclusively to this region of the GP Ib-IX complex.
New synthesis of jasmine lactone and related .delta.-lactones from 1,2-cyclohexanedione. Preparation and dye-sensitized photooxygenation of 3-(2-alkenyl)- and 3-(2-alkynyl)-1,2-cyclohexanediones (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew synthesis of jasmine lactone and related .delta.-lactones from 1,2-cyclohexanedione. Preparation and dye-sensitized photooxygenation of 3-(2-alkenyl)- and 3-(2-alkynyl)-1,2-cyclohexanedionesMasanori Utaka, Hidesuke Kuriki, Takashi Sakai, and Akira TakedaCite this: J. Org. Chem. 1986, 51, 6, 935–938Publication Date (Print):March 1, 1986Publication History Published online1 May 2002Published inissue 1 March 1986https://pubs.acs.org/doi/10.1021/jo00356a035https://doi.org/10.1021/jo00356a035research-articleACS PublicationsRequest reuse permissionsArticle Views401Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
[Perioperative anxiety and postoperative pain suppression in intraocular operations using general anesthesia and local anesthesia]. (1988)

The scores of state and trait anxiety behavior are comparable to a normal geriatric population, even if normal data cannot always be applied to clinical situations.(ABSTRACT TRUNCATED AT 250 WORDS)
Role of purified erythropoietin in the amplification of the erythroid compartment. (1980)

A single dose of Myleran induced a prolonged depletion of multi-potential haemopoietic cells and of early erythroid precursors to levels between 2 and 6 of control. In these mice, a priming dose of highly purified erythropoietin (Epo) or of any of 3 Epo preparations from different sources, and having different specific activities, increased the magnitude of the response to a test dose of Epo by erythropoietin responsive cells (ERC) in the hypertransfused mouse assay. As significant feeding into the ERC compartment from the most immature cells is unlikely because of the depletion induced by the Myleran, it may be concluded that highly purified Epo, not contaminated with other biologically active molecules, has a dual effect on the ERC: it causes increased amplification besides inducing differentiation into cells with the capacity to synthesize haemoglobin.
Phenotypic rescue of mutant brown melanocytes by a retrovirus carrying a wild-type tyrosinase-related protein gene (1990)

A mouse cDNA for the developmentally controlled, melanocyte-specific protein, tyrosinase-related protein 1 (TRP-1), was previously cloned and reported to show genetic linkage with the coat-colour locus brown (b) on mouse chromosome 4. The cDNA has been inserted into a retroviral vector derived from Moloney murine leukaemia virus, under the control of the human histone H4 promoter. This vector was used to infect melanocytes of the immortal line melan-b, which are homozygous for the b mutation and which display light brown pigmentation in culture. Infected cultures containing between 0.2 and 2 copies of provirus per cell displayed an altered phenotype: 20-50% of cells now had the black to dark brown colour characteristic of cultured wild-type (Black, B/B) mouse melanocytes. Thus the TRP-1 gene complements the brown mutation. We conclude that TRP-1 is the product of the wild-type b-locus.
An MPEG-2 encoder architecture based on a single-chip dedicated LSI with a control MPU (2002)

This paper describes an MPEG-2 encoder architecture based on a hard-wired LSI with a control MPU. All basic functions of MPEG-2 MP@ML video compression are integrated in the dedicated LSI. For the motion estimation, a horizontally subsampled, diamond search was employed as a simplified first search step. It can reduce operations to 20% of the full-search, with an estimated SNR degradation of only -0.1 dB. To help achieve a single-memory interface, a pair of 81 MHz, 16 Mb SDRAMs are used as a frame buffer and a code buffer. Data bandwidth between the SDRAMs and the LSI is kept to less than 94% of the maximum data rate. Jobs assigned to the control MPU need be executed less frequently than those of the macroblock coding, which helps reduce the requirements for MPU performance to about 7 MIPS.
Grating-flanked plasmonic coaxial apertures for efficient fiber optical tweezers (2016)

Subwavelength plasmonic apertures have been foundational for direct optical manipulation of nanoscale specimens including sub-100 nm polymeric beads, metallic nanoparticles and proteins. While most plasmonic traps result in two-dimensional localization, three-dimensional manipulation has been demonstrated by integrating a plasmonic aperture on an optical fiber tip. However, such 3D traps are usually inefficient since the optical mode of the fiber and the subwavelength aperture only weakly couple. In this paper we design more efficient optical-fiber-based plasmonic tweezers combining a coaxial plasmonic aperture with a plasmonic grating coupler at the fiber tip facet. Using full-field finite difference time domain analysis, we optimize the grating design for both gold and silver fiber-based coaxial tweezers such that the optical transmission through the apertures is maximized. With the optimized grating, we show that the maximum transmission efficiency increases from 2.5% to 19.6% and from 1.48% to 16.7% for the gold and silver structures respectively. To evaluate their performance as optical tweezers, we calculate the optical forces and the corresponding trapping potential on dielectric particles interacting with the apertures. We demonstrate that the enahncement in the transmission translates into an equivalent increase in the optical forces. Consequently, the optical power required to achieve stable optical trapping is significantly reduced allowing for efficient localization and 3D manipulation of sub-30 nm dielectric particles.
Evaluation of fully 3-D emission mammotomography with a compact cadmium zinc telluride detector (2005)

A compact, dedicated cadmium zinc telluride (CZT) gamma camera coupled with a fully three-dimensional (3-D) acquisition system may serve as a secondary diagnostic tool for volumetric molecular imaging of breast cancers, particularly in cases when mammographic findings are inconclusive. The developed emission mammotomography system comprises a medium field-of-view, quantized CZT detector and 3-D positioning gantry. The intrinsic energy resolution, sensitivity and spatial resolution of the detector are evaluated with Tc-99m (140 keV) filled flood sources, capillary line sources, and a 3-D frequency-resolution phantom. To mimic realistic human pendant, uncompressed breast imaging, two different phantom shapes of an average sized breast, and three different lesion diameters are imaged to evaluate the system for 3-D mammotomography. Acquisition orbits not possible with conventional emission, or transmission, systems are designed to optimize the viewable breast volume while improving sampling of the breast and anterior chest wall. Complications in camera positioning about the patient necessitate a compromise in these two orbit design criteria. Image quality is evaluated with signal-to-noise ratios and contrasts of the lesions, both with and without additional torso phantom background. Reconstructed results indicate that 3-D mammotomography, incorporating a compact CZT detector, is a promising, dedicated breast imaging technique for visualization of tumors < 1 cm in diameter. Additionally, there are no outstanding trajectories that consistently yield optimized quantitative lesion imaging parameters. Qualitatively, imaging breasts with realistic torso backgrounds (out-of-field activity) substantially alters image characteristics and breast morphology unless orbits which improve sampling are utilized. In practice, the sampling requirement may be less strict than initially anticipated.
Fluorescent Imaging Applications of Quantum Dot Probes (2007)

Quantum dots (QDs) have rapidly emerged as an attractive alternative to conventional organic fluorophores in a variety of biological imaging applications. Their improved photostability allows for long-term dynamic imaging of cellular processes and their narrow, size-tunable emission permits unprecedented multiplexing capabilities. Additionally, the inherent brightness of core/shell QDs, with quantum yields capable of exceeding 85%, provides increased sensitivity for both diagnostic screening and single molecule tracking applications. To date, the primary focus of research in this field has been directed towards modifying the surface chemistries of the QDs to introduce biological specificity while, at the same time, limiting nonspecific cellular interactions. As such, biomolecules such as antibodies, peptides, streptavidin and biotin have all been conjugated to QDs and been used to demonstrate specific labeling of cellular targets. Additionally, polyethylene glycol (PEG) modification of the QD surface has been shown to limit nonspecific interactions. The use of small molecule QD-conjugates has also been demonstrated as an effective means for targeted labeling of membrane associated receptors. This approach introduces specificity via ligand-receptor interactions, resulting in a highly modular system which is easily modified to interrogate a wide variety of cellular targets. This article provides a comprehensive review of the current status of QD imaging applications in biological systems with a particular emphasis on the design and application of small molecule nanoconjugates.
Significance of IGFBP-4 in the Development of Fetal Growth Restriction (2012)

Our findings support the role of IGFBP-4 in regulating IGF bioavailability and provide new clues for the prevention and treatment of FGR, raising the possibility of clinical use of IGFBP-4 as an early biomarker for this condition.
Molecule-Mimetic Chemistry and Mesoscale Self-Assembly (2000)

Molecules are structured aggregates of atoms joined by chemical bonds; crystals are aggregates of molecules, interacting covalently or noncovalently. The work described in this Account uses molecules, crystals, and other forms of atomic/molecular matter to suggest principles that can be used in generating structured aggregates of millimeter-scale components, interacting through capillary interactions. The properties of these aggregates--that is, their "chemistry"--mimic aspects of the chemistry of molecules.
Pairwise Independence and Derandomization (2005)

Pairwise Independence and Derandomization gives several applications of the following paradigm, which has proven extremely powerful in algorithm design and computational complexity. First, design a probabilistic algorithm for a given problem. Then, show that the correctness analysis of the algorithm remains valid even when the random strings used by the algorithm do not come from the uniform distribution, but rather from a small sample space, appropriately chosen. In some cases this can be proven directly (giving "unconditional derandomization"), and in others it uses computational assumptions, like the existence of 1-way functions (giving "conditional derandomization"). Pairwise Independence and Derandomization is self contained, and is a prime manifestation of the "derandomization" paradigm. It is intended for scholars and graduate students in the field of theoretical computer science interested in randomness, derandomization and their interplay with computational complexity.
Novel gene transcripts preferentially expressed in human muscles revealed by quantitative hybridization of a high density cDNA array. (1996)

A set of 1091 human skeletal muscle cDNA clone inserts representing more than 800 human gene transcripts were spotted as PCR products at high density on nylon membranes. Replicas of the filters were hybridized in stringent conditions with 33P-radiolabeled cDNA probes transcribed from skeletal muscle poly(A)+ RNA. Hybridization signals were collected on phosphor screens and processed using a software specifically adapted for this application to identify and quantitate each spot. Parameters likely to influence the hybridization signal intensity were assessed to eliminate artifacts. Each clone was assigned to one of four intensity classes reflecting the steady-state level of transcription of the corresponding gene in skeletal muscle. Differential expression of specific gene transcripts was detected using complex cDNA probes derived from nine different tissues, allowing assessment of their tissue specificity. This made it possible to identify 48 novel gene transcripts (including 7 homologous or related to known sequences) with a muscle-restricted pattern of expression. These results were validated through the analysis of known muscle-specific transcripts and by Northern analysis of a subset of the novel gene transcripts. All these genes have been registered in the Genexpress Index, such that sequence, map, and expression data can be used to decipher their role in the physiology and pathology of human muscles.
Retroviral-mediated gene transfer into hepatocytes in vivo. (1991)

Stable gene transfer into hepatocytes might be used to compensate for a genetic deficiency affecting liver function or to deliver diffusible factors into the blood stream. In rats, we have combined retroviral-mediated gene transfer with a surgical procedure in which the liver is temporarily excluded from the circulation and infected in vivo. Partial hepatectomy was performed 24-48 hr before perfusion with virus to induce hepatocyte division and facilitate viral integration. A helper-free recombinant retrovirus coding for beta-galactosidase with nuclear localization was used to score cells that expressed the transgene. For at least 3 months after gene transfer, up to 5% of hepatocytes expressed nuclear beta-galactosidase. Whereas in vitro reimplantation of genetically modified hepatocytes has proved to be inefficient in stably transferring genes into the liver, our approach provides a feasible alternative.
(Bis)urea and (Bis)thiourea Inhibitors of Lysine-Specific Demethylase 1 as Epigenetic Modulators (2010)

The recently discovered enzyme lysine-specific demethylase 1 (LSD1) plays an important role in the epigenetic control of gene expression, and aberrant gene silencing secondary to LSD1 overexpression is thought to contribute to the development of cancer. We recently reported a series of (bis)guanidines and (bis)biguanides that are potent inhibitors of LSD1 and induce the re-expression of aberrantly silenced tumor suppressor genes in tumor cells in vitro. We now report a series of isosteric ureas and thioureas that are also potent inhibitors of LSD1. These compounds induce increases in methylation at the histone 3 lysine 4 (H3K4) chromatin mark, a specific target of LSD1, in Calu-6 lung carcinoma cells. In addition, these analogues increase cellular levels of secreted frizzle-related protein (SFRP) 2 and transcription factor GATA4. These compounds represent an important new series of epigenetic modulators with the potential for use as antitumor agents.
Decreased STAT4 indicates poor prognosis and enhanced cell proliferation in hepatocellular carcinoma (2015)

Our data indicate that STAT4 may inhibit HCC development by modulating HCC cell proliferation.
Rats Expressing Human Cytosolic Copper–Zinc Superoxide Dismutase Transgenes with Amyotrophic Lateral Sclerosis: Associated Mutations Develop Motor Neuron Disease (2001)

Some cases of familial amyotrophic lateral sclerosis (ALS) are caused by mutations in the gene encoding cytosolic, copper-zinc superoxide dismutase (SOD1). We report here that rats that express a human SOD1 transgene with two different ALS-associated mutations (G93A and H46R) develop striking motor neuron degeneration and paralysis. As in the human disease and transgenic ALS mice, pathological analysis demonstrates selective loss of motor neurons in the spinal cords of these transgenic rats. In spinal cord tissues, this is accompanied by activation of apoptotic genes known to be activated by mutant SOD1 protein in vitro and in vivo. These animals provide additional support for the proposition that motor neuron death in SOD1-related ALS reflects one or more acquired, neurotoxic properties of the mutant SOD1 protein. The larger size of this rat model as compared with the ALS mice will facilitate studies involving manipulations of spinal fluid (implantation of intrathecal catheters for chronic therapeutic studies; CSF sampling) and spinal cord (e.g., direct administration of viral- and cell-mediated therapies).
Position-independent expression of the ovine β-lactoglobulin gene in transgenic mice (1992)

The major milk whey protein of sheep, beta-lactoglobulin (BLG), is expressed specifically in the mammary gland in a developmentally regulated pattern. To identify the cis-acting DNA regions involved in the regulation of BLG expression, resected gene constructs were analysed in transgenic mice. BLG transgenes which contain at least the proximal 406 bp of the 5' flanking region were expressed in all mice analysed, at levels related to transgene copy number, and thus were expressed in a position-independent manner. Expression was restricted to the mammary gland, except in a few lines where low-level expression was also detected in the salivary gland. In these mice, BLG transgenes were expressed during pregnancy and lactation in the appropriate temporal pattern. Further resection of the 5' proximal region to -146 bp resulted in a dramatically reduced frequency of expression, without affecting tissue specificity, while a construct which retained only 79 bp of 5' flanking region was not expressed. Chromatin analysis of isolated sheep nuclei showed that the promoter resides within a DNAaseI-hypersensitive region in the mammary gland but not in the liver. A BLG transgene displayed a similar tissue-specific pattern of DNAaseI hypersensitivity in mice. These data demonstrate an essential role of the proximal DNAaseI-hypersensitive sequences for position-independent expression of the BLG gene.
Unforgettable Songs of Experience: Women's Global Experiences of Violence a Report from the 12th World Congress on Criminology, Seoul, South Korea, August 1998 (1999)

In August 1998 I attended the 12th World Congress of Criminology, in Seoul, South Korea to present a work-in-progress paper on my doctoral research. Topic areas at the congress included world perspectives on marital violence, violence against women and children, and family violence. Reading through the program of presenters it was surprising and disappointing to discover that aside from myself, no other Australian was presenting a paper on domestic violence or women's and children's experiences of violence in Australia. I was presenting a paper on my doctoral research pertaining to the legal construction of victim/survivors of intrafamilial sexual abuse in Victorian County Court trials during 1995. What I would like to present in this article is a brief glance through the windows of women's global experiences of violence as articulated by speakers from around the world.