gebhart/patrepeval huggingface dataset:
Automated diagnosis and disease characterization using neural network analysis (2003)

A neural network approach is used to analyze and diagnose a rather new and uncommon disease, Lyme borreliosis. To fully exploit the method's generalizing power, a significance analysis split the set of inputs of a trained network into two classes that were important and unimportant. The results of this analysis lead to a new structured network, whose topology and architecture reflect the estimated relevance of symptoms. The diagnostic performance thus obtained showed a dramatic improvement which reached an average error rate of around 6%.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
One-Dimensional Nanostructures of Metals:  Large-Scale Synthesis and Some Potential Applications (2007)

We review recent developments in our group regarding the solution-phase synthesis of one-dimensional nanostructures of metals. The synthetic approaches include solution-liquid-solid growth for nanowires of low-melting-point metals such as Pb; seed-directed growth for Ag nanowires, nanobeams, and nanobelts; kinetically controlled growth for Pt nanorods, nanowires, and multipods; and galvanic replacement for nanotubes of Au, Pt, and Pd. Both characterization and mechanistic studies are presented for each nanostructure. Finally, we highlight the electrical and plasmonic properties of these metal nanostructures and discuss their potential applications in nanoscale devices.
Managing Diversity in Regression Ensembles (2005)

Ensembles are a widely used and effective technique in machine learning---their success is commonly attributed to the degree of disagreement, or 'diversity', within the ensemble. For ensembles where the individual estimators output crisp class labels, this 'diversity' is not well understood and remains an open research issue. For ensembles of regression estimators, the diversity can be exactly formulated in terms of the covariance between individual estimator outputs, and the optimum level is expressed in terms of a bias-variance-covariance trade-off. Despite this, most approaches to learning ensembles use heuristics to encourage the right degree of diversity. In this work we show how to explicitly control diversity through the error function. The first contribution of this paper is to show that by taking the combination mechanism for the ensemble into account we can derive an error function for each individual that balances ensemble diversity with individual accuracy. We show the relationship between this error function and an existing algorithm called negative correlation learning, which uses a heuristic penalty term added to the mean squared error function. It is demonstrated that these methods control the bias-variance-covariance trade-off systematically, and can be utilised with any estimator capable of minimising a quadratic error function, for example MLPs, or RBF networks. As a second contribution, we derive a strict upper bound on the coefficient of the penalty term, which holds for any estimator that can be cast in a generalised linear regression framework, with mild assumptions on the basis functions. Finally we present the results of an empirical study, showing significant improvements over simple ensemble learning, and finding that this technique is competitive with a variety of methods, including boosting, bagging, mixtures of experts, and Gaussian processes, on a number of tasks.
Packet communication protocol for image services on a high-speed multimedia LAN (1989)

The authors describe the requirements for high-speed multimedia communication and propose a high-speed communication protocol to provide congestion-free access and efficient retransmission/flow control. Its usefulness is proved in a 400 Mb/s multiaccess loop local area network (LAN) with a 100 Mb/s user interface. The main characteristics of the protocol are separation of image communication handling, guarantee of no buffer overflow in a network, and end-to-end block-based transfer. Buffer reservation control in the user-network interface and a retransmission scheme based on a long-size block are used to realize high-speed congestion control and error recovery. A multimedia terminal architecture suitable for real-time image communication is also discussed. In the prototype system, a few frames of high-resolution image information can be transferred in a second. Around 26 Mb/s effective throughput between application entities has been obtained.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
[Expression of cytokines and interferon-related genes in the mouse embryo]. (1994)

The expression of the cytokine and IFN-related genes was studied in mouse embryo using RT-PCR method capable of detecting low levels of mRNA. Total RNA was prepared from the days 7 embryos by acid guanidinium thiocyanate-phenol-chloroform (AGPC) method. cDNA was synthesized by M-MLV reverse transcriptase, and amplified using the specific oligonucleotide primers for IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-alpha, IFN-alpha, IFN-beta, IFN-gamma, IFN-alpha/beta receptor (IFN-alpha/beta R), IFN-gamma receptor (IFN-gamma R), interferon reguratory factor (IRF)-1, IRF-2 and 2'-5' oligoadenylate synthetase (2-5AS) by PCR method. Although the expressions of IL-1 beta, IL-4, IL-5, IL-6, TNF-alpha and IFN-gamma mRNA were detected in all the embryos tested, the expressions of IL-2, IL-3, IFN-alpha and IFN-beta mRNA were not detected at all. On the other hand, the expressions of IFN-related genes such as IFN-alpha/beta R, IFN-gamma R, IRF-1, IRF-2 and 2-5AS mRNA, were also detected. These results suggest that these cytokine may play an important role in early embryonic development.
Characterization of 3-D imaging lidar for hazard avoidance and autonomous landing on the Moon (2007)

Future robotic and crewed lunar missions will require safe and precision soft-landing at scientifically interesting sites near hazardous terrain features such as craters and rocks or near pre-deployed assets. Presently, NASA is studying the ability of various 3-dimensional imaging sensors particularly lidar/ladar techniques in meeting its lunar landing needs. For this reason, a Sensor Test Range facility has been developed at NASA Langley Research Center for calibration and characterization of potential 3-D imaging sensors. This paper describes the Sensor Test Range facility and its application in characterizing a 3-D imaging ladar. The results of the ladar measurement are reported and compared with simulated image frames generated by a ladar model that was also developed as part of this effort. In addition to allowing for characterization and evaluation of different ladar systems, the ladar measurements at the Sensor Test Range will support further advancement of ladar systems and development of more efficient and accurate image reconstruction algorithms.
Noncoupling Synthesis of Unsymmetrical Tetrathiafulvalene Derivatives. Dihydrotetrathiafulvalenes as New Electron Donors (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNoncoupling Synthesis of Unsymmetrical Tetrathiafulvalene Derivatives. Dihydrotetrathiafulvalenes as New Electron DonorsJun-ichi Yamada, Yoshiki Amano, Satoshi Takasaki, Rie Nakanishi, Kaoru Matsumoto, Shyuji Satoki, and Hiroyuki AnzaiCite this: J. Am. Chem. Soc. 1995, 117, 3, 1149–1150Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ja00108a040https://doi.org/10.1021/ja00108a040research-articleACS PublicationsRequest reuse permissionsArticle Views218Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Plasticity of the Intrinsic Period of the Human Circadian Timing System (2007)

Human expeditions to Mars will require adaptation to the 24.65-h Martian solar day-night cycle (sol), which is outside the range of entrainment of the human circadian pacemaker under lighting intensities to which astronauts are typically exposed. Failure to entrain the circadian time-keeping system to the desired rest-activity cycle disturbs sleep and impairs cognitive function. Furthermore, differences between the intrinsic circadian period and Earth's 24-h light-dark cycle underlie human circadian rhythm sleep disorders, such as advanced sleep phase disorder and non-24-hour sleep-wake disorders. Therefore, first, we tested whether exposure to a model-based lighting regimen would entrain the human circadian pacemaker at a normal phase angle to the 24.65-h Martian sol and to the 23.5-h day length often required of astronauts during short duration space exploration. Second, we tested here whether such prior entrainment to non-24-h light-dark cycles would lead to subsequent modification of the intrinsic period of the human circadian timing system. Here we show that exposure to moderately bright light ( approximately 450 lux; approximately 1.2 W/m(2)) for the second or first half of the scheduled wake episode is effective for entraining individuals to the 24.65-h Martian sol and a 23.5-h day length, respectively. Estimations of the circadian periods of plasma melatonin, plasma cortisol, and core body temperature rhythms collected under forced desynchrony protocols revealed that the intrinsic circadian period of the human circadian pacemaker was significantly longer following entrainment to the Martian sol as compared to following entrainment to the 23.5-h day. The latter finding of after-effects of entrainment reveals for the first time plasticity of the period of the human circadian timing system. Both findings have important implications for the treatment of circadian rhythm sleep disorders and human space exploration.
Single channel study of a Ca(2+)‐activated K+ current associated with ras‐induced cell transformation. (1993)

1. Ras-transformed fibroblasts have a whole-cell Ca(2+)-activated K+ current which is either absent or unavailable for activation in their non-transformed counterparts. To better understand the physiological significance of this K+ current the single channel basis for the current was characterized in ras-transformed cells. 2. More than 90% of inside-out patches from ras-transformed balb 3T3 cells had a channel type which was Ca(2+)-activated (threshold < 0.2 microM internal Ca2+), K(+)-selective (permeability ratio PNa:PK < 0.02), and inwardly rectifying in symmetric 150 mM KCl solutions (conductances at -60 and 60 mV of 33 +/- 1 and 17 +/- 1 pS respectively). Channel opening probability increased 25-50% between -60 and 60 mV due to an increase in the frequency of opening. Single K+ channels in outside-out patches were blocked by externally applied 10 mM TEA or 100 nM charybdotoxin, as were whole-cell Ca(2+)-activated K+ currents. The properties of this class of K+ channel are sufficient to account for the whole-cell Ca(2+)-activated current in ras-transformed cells. 3. Inside-out patches from C3H10T1/2 and NIH 3T3 fibroblasts transformed by the H-ras oncogene had Ca(2+)-activated K+ channels identical to those observed in K-ras-transformed balb 3T3 cells. 4. As predicted from whole-cell experiments Ca(2+)-activated K+ channels were not observed in inside-out patches from non-transformed balb 3T3 cells. The purpose of the excised patch recordings was, instead, to rule out potential technical complications with the whole-cell experiments. For instance A23187, which evoked whole-cell K+ currents in transformed cells, may not have elevated Ca2+ sufficiently to allow K+ channel activation in non-transformed cells. Another possibility was that trypsin pretreatment used to round-up cells for whole-cell recording may have preferentially disabled channels in non-transformed cells. The first problem was addressed by exposing patches from non-transformed cells to 100-1000 microM Ca2+. Excised patches were also taken from non-transformed cells which had not been exposed to trypsin. K+ channel activity was not observed under either condition. 5. Patches from both ras-transformed and non-transformed cells had a type of non-specific cation channel which was activated at internal Ca2+ concentrations > or = 100 microM. This channel was sensitive to membrane voltage, mean open time increasing from 12 to 72 ms between -90 and 90 mV.
A trial of dextromethorphan in parkinsonian patients with motor response complications (1998)

The effects of the NMDA antagonist dextromethorphan (DM) on levodopa-associated dyskinesias and motor fluctuations were studied in patients with advanced Parkinson's disease. During initial open-label dose escalation, 6 of 18 patients reported a beneficial effect at their individually determined optimal DM dose (range, 60-120 mg/day). The 12 remaining patients either experienced reversible side effects, particularly mild drowsiness, or decreased levodopa efficacy, and were therefore excluded from the study. The six responders entered the double-blind, placebo-controlled, crossover study with two 2-week arms separated by 1 week wash-out. On the last day of each arm, motor ratings were performed every 20 minutes for 8 consecutive hours. In addition, motor complications and Activities of Daily Living (ADL) were assessed using the Unified Parkinson's Disease Rating Scale (UPDRS) and patient diaries. With DM, dyskinesias improved by 25% according to physician's ratings and by 40% according to UPDRS interviews, without compromising the anti-Parkinson effect of levodopa. Motor fluctuations and ADL scores also improved significantly. Although the narrow therapeutic index of DM limits its clinical usefulness, these findings support the view that drugs acting to inhibit glutamatergic transmission at the NMDA receptor can ameliorate levodopa-associated motor complications.
Development and Characterization of Monoclonal Antibodies to Detect Klotho (2014)

Although antibodies are commercially available to allow investigation into the biology of the age-regulating protein Klotho, problems with antibody specificity and application functionality are significant barriers to progress. Chief among these limitations is the inability of current tools to allow in vivo validation of binding partners originally identified through transfection of tagged proteins. To overcome this barrier, we generated a series of hybridoma cell lines by immunizing rats with a GST-KL1 fusion protein. Purified antibodies generated from these cell lines differentially detect human or mouse Klotho protein via Western blot, immunocyto/histochemistry, and immunoprecipitation. Specificity of antibody binding to Klotho was confirmed by mass spectrometry following immunoprecipitation. With this confidence in antibody specificity, co-immunoprecipitation was utilized to validate the interaction of Klotho/FGFR and Klotho/wnt7a in mouse kidney lysates.
STAT3β, a Splice Variant of Transcription Factor STAT3, Is a Dominant Negative Regulator of Transcription (1996)

The 89-kDa STAT3 protein is a latent transcription factor which is activated in response to cytokines (interleukin (IL)-5 and -6) and growth factors (epidermal growth factor). Binding of IL-5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Here we report the cloning of a cDNA encoding a variant of the transcription factor STAT3 (named STAT3beta) which was isolated by screening an eosinophil cDNA library. Compared to wild-type STAT3, STAT3beta lacks an internal domain of 50 base pairs located near the C terminus. This splice product is a naturally occurring isoform of STAT3 and encodes a 80-kDa protein. We found by reconstitution of the human IL-5R in COS cells that like STAT3, STAT3beta is phosphorylated on tyrosine and binds to the pIRE from the ICAM-1 promoter after IL-5 stimulation. However, STAT3beta fails to activate a pIRE containing promoter in transient transfection assays. Instead, co-expression of STAT3beta inhibits the transactivation potential of STAT3. These results suggests that STAT3beta functions as a negative regulator of transcription.
Factors Affecting the Production of Fast Transient Signals in Single Shot Laser Ablation Inductively Coupled Plasma Mass Spectrometry (2002)

Low sample consumption and high achievable spatial resolution combine to make laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) an attractive direct sampling technique for the analysis of solids. These desirable characteristics are best realized when the laser ablation system is operated in a single-shot fashion, in which each laser pulse produces a transient analyte signal. The temporal width of a single-shot LA analyte transient is inversely related to the best achievable signal-to-noise ratio (S/N). Thus, production of fast transient signals is an important consideration in single-shot LA analyses. In this study we explore the effects on transient pulse width of ablation-cell volume, the diameter and length of tubing used to connect the ablation cell to the ICP, and the composition and flow rate of ablation-cell sweep gas. Minimization of ablation-cell volume and the length and diameter of the transfer tube was found to dramatically decrease the peak widths of transient signals. However, use of helium gas to sweep analyte particles from the ablation cell was found to significantly reduce the effect of cell volume on transient width. Use of a cell volume of 0.70 cm 3 and optimization of other instrumental parameters produced a transient sample pulse 85 ms in duration and limits of detection in the tens of femtograms range for single-shot laser-ablation events.
Macromol. React. Eng. 2/2011 (2011)

Cover: Polymer microstructure control in latex-based pressure sensitive adhesives (PSAs) leads to properties comparable to that in solvent-based PSAs. The normally discrete gel structure in polymer latex films is converted to a continuous network structure as found in solvent-based polymer films. Further details can be found in the article by L. Qie and M. A. Dubé* on page 117.
MK-801 prevents hypobaric-ischemic neuronal degeneration in infant rat brain (1989)

Recent evidence implicates the endogenous excitatory amino acids, glutamate (Glu) and aspartate, in hypoxic/ischemic neuronal degeneration. In a preceding article (Ikonomidou et al., 1989) we described a new model for studying hypoxic/ischemic neuronal degeneration in the infant rat brain that entails unilateral common carotid artery ligation followed by exposure to a partial vacuum for 75 min. Promising features of this model include a low mortality rate and high incidence of acute brain damage disseminated over numerous brain regions. In addition, there is a striking similarity between the type of cytopathology characterizing this model of hypoxic/ischemic neuronal degeneration and that which has been described in infant animals treated with Glu. MK-801 is a powerful antagonist of the N-methyl-D-aspartate (NMDA) receptor ionophore complex (a subtype of Glu receptor). In the present study, after unilateral carotid artery ligation was performed on 10-d-old rat pups, they were treated either with MK-801 (1 mg/kg i.p.) or saline 15 min before exposure to the hypobaric condition. MK-801 exerted a strong neuroprotective effect without serious side effects; the majority of saline control animals sustained severe brain damage, whereas the majority of MK-801-treated pups had no brain damage. These and other recent findings suggest that the NMDA receptor may play an important role in hypoxic/ischemic neuronal degeneration in the immature brain and provide hope that NMDA antagonists such as MK-801 may be effective in preventing such degeneration.
Crystal Structure of Human Toll-Like Receptor 3 (TLR3) Ectodomain (2005)

Toll-like receptors (TLRs) play key roles in activating immune responses during infection. The human TLR3 ectodomain structure at 2.1 angstroms reveals a large horseshoe-shaped solenoid assembled from 23 leucine-rich repeats (LRRs). Asparagines conserved in the 24-residue LRR motif contribute extensive hydrogen-bonding networks for solenoid stabilization. TLR3 is largely masked by carbohydrate, but one face is glycosylation-free, which suggests its potential role in ligand binding and oligomerization. Highly conserved surface residues and a TLR3-specific LRR insertion form a homodimer interface in the crystal, whereas two patches of positively charged residues and a second insertion would provide an appropriate binding site for double-stranded RNA.
Naloxone Suppresses Buprenorphine Stimulation of Plasma Prolactin (1989)

The effects of parenterally-administered buprenorphine and simultaneous injection of naloxone was evaluated in six healthy adult males. Each subject was studied on six occasions, an average of 10 days apart, and received either two simultaneous intramuscular injections of saline, buprenorphine 0.3 mg and saline, or buprenorphine 0.3 mg and 0.6 mg, 0.45 mg, 0.3 mg, or 0.15 mg of naloxone. Simultaneous injection of buprenorphine 0.3 mg and saline resulted in an average increase in plasma prolactin above baseline levels of approximately 10 and 25 ng/ml, 30 and 55 minutes after injection. Buprenorphine-induced stimulation of plasma prolactin levels was statistically significantly greater than basal prolactin values (p less than 0.01). When 0.6 mg of naloxone was simultaneously injected with 0.3 mg buprenorphine, peak plasma prolactin levels were significantly lower (p less than 0.05) than prolactin values after administration of 0.3 mg buprenorphine and saline. Simultaneous injection of 0.45 mg naloxone and 0.3 mg buprenorphine also resulted in a significant attenuation (p less than 0.05) of buprenorphine-stimulated prolactin levels. Injection of 0.3 mg or 0.15 mg of naloxone did not inhibit prolactin stimulation produced by buprenorphine 0.3 mg. These findings demonstrate a dose-effect relationship between naloxone concentration and suppression of the increase in plasma prolactin levels produced by administration of buprenorphine 0.3 mg. As prolactin stimulation occurs shortly after opioid agonist administration and is temporally concordant with the rapid induction of pharmacologic reinforcement associated with opiate abuse, naloxone added to buprenorphine parenteral preparations may reduce the abuse potential of buprenorphine.