gebhart/patrepeval huggingface dataset:
Humanized Mik beta 1, a humanized antibody to the IL-2 receptor beta -chain that acts synergistically with humanized anti-TAC. (1993)

Mik beta 1 is a mouse mAb directed at the beta-subunit of the human IL-2R (Tac) that inhibits IL-2 binding and inhibits IL-2 induction of large granular lymphocytes (LGL). Mik beta 1 alone does not inhibit IL-2-induced T-cell proliferation, but acts synergistically with anti-Tac to inhibit IL-2-induced proliferation of activated T cells. To evaluate these effects for possible therapy in humans, we constructed two humanized Mik beta 1 antibodies by combining the complementarity-determining regions of the murine antibody with human framework and constant regions. Compared with murine Mik beta 1, the two humanized Mik beta 1 antibodies, which differ in their degree of humanization, had similar affinities for IL-2R beta. The murine Mik beta 1 and one of the humanized Mik beta 1 antibodies were equivalent in competing for IL-2 binding to IL-2R beta and inhibiting IL-2 induction of LGL cytotoxicity. The activity of the second humanized antibody was significantly reduced. The three Mik beta 1 antibodies act synergistically to varying degrees with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells. This capacity to synergize paralleled their abilities to inhibit IL-2 binding. In addition, both humanized antibodies directed antibody-dependent cell-mediated cytotoxicity. We hope that humanized Mik beta 1 in combination with humanized anti-Tac will provide a new immunosuppressive therapy for the treatment of autoimmune diseases, graft-versus-host disease, and prevention of allograft rejection.
Ovonic unified memory - a high-performance nonvolatile memory technology for stand-alone memory and embedded applications (2005)

The development status of Ovonic Unified Memory (OUM), a phase-change non-volatile semiconductor memory technology for VLSI stand-alone memory and embedded applications, is discussed. Using 0.18 μm 3 V CMOS, cells from 5F/sup 2/ to 8F/sup 2/ are built in a charge-pump-free 4 Mb development vehicle. Direct overwrite, 10 ns reset times, 50 ns set times, and 1.0×10/sup 12/ cycling are achieved. At en-year data retention is projected at 120°C.
Erythropoiesis in the Chick Embryo: The Role of Endoderm (1965)

The endoderm can be separated from the ectomesoderm of the area vasculosa of the early chick embryo. The development of the separated germ layers in organ cultures shows that the endoderm is necessary for the normal formation of blood islands by mesodermal cells. In the absence of endoderm the mesodermal cells undergo erythropoiesis in dispersed groups of cells but do not form endothelium.
Synthesis of 3-(4-acylaminopiperazin-1-ylalkyl)indoles as potential antihypertensive agents (1977)

Controlling the intensity and manipulating the spectral composition of sunlight are critical for many devices including “smart” windows, greenhouses, and photomicroreactors, but these are also important in more decorative applications. Here, we use a diarylethene dye incorporated in a liquid crystal host to create a dual-responsive “smart” window regulated both by an electrical trigger and by specific wavelengths of light. By incorporating the same diarylethene dye in a polymerizable host and using inkjet printing, coatings can be made with complete freedom in the applied pattern design, although the electrical response is lost. The color change of the diarylethene dye can be controlled in simulated sunlight by concurrent light exposure from an LED source, allowing a manual override for outdoor use. Photoluminescence of the closed isomer of the diarylethene from the light guide edges could be used for lighting or electricity generation in a luminescent solar concentrator architecture.
Smn, the spinal muscular atrophy–determining gene product, modulates axon growth and localization of β-actin mRNA in growth cones of motoneurons (2003)

Spinal muscular atrophy (SMA), a common autosomal recessive form of motoneuron disease in infants and young adults, is caused by mutations in the survival motoneuron 1 (SMN1) gene. The corresponding gene product is part of a multiprotein complex involved in the assembly of spliceosomal small nuclear ribonucleoprotein complexes. It is still not understood why reduced levels of the ubiquitously expressed SMN protein specifically cause motoneuron degeneration. Here, we show that motoneurons isolated from an SMA mouse model exhibit normal survival, but reduced axon growth. Overexpression of Smn or its binding partner, heterogeneous nuclear ribonucleoprotein (hnRNP) R, promotes neurite growth in differentiating PC12 cells. Reduced axon growth in Smn-deficient motoneurons correlates with reduced beta-actin protein and mRNA staining in distal axons and growth cones. We also show that hnRNP R associates with the 3' UTR of beta-actin mRNA. Together, these data suggest that a complex of Smn with its binding partner hnRNP R interacts with beta-actin mRNA and translocates to axons and growth cones of motoneurons.
Requirement of Nuclear Factor of Activated T-cells in Calcineurin-mediated Cardiomyocyte Hypertrophy (2002)

The calcium-activated phosphatase calcineurin has been implicated as a critical intracellular signal transducer of cardiomyocyte hypertrophy. Although previous data suggested the nuclear factor of activated T-cells (NFAT) as its sole transcriptional effector, the absolute requirement of NFAT as a mediator of calcineurin signaling has not been examined in the heart. We therefore investigated the expression and activation profile of NFAT genes in the heart. Four members (NFATc1-c4) are expressed in cardiomyocytes, elicit nuclear translocation upon calcineurin activation, and are able to drive transactivation of cardiac promoter luciferase constructs. To define the necessary function of NFAT factors as hypertrophic transducers, a dominant negative NFAT construct was created, encompassing part of the N-terminal region of NFATc4 containing a conserved calcineurin-binding motif. Cotransfection of this construct dose-dependently abrogated promoter activation, irrespective of the NFAT isoform used, whereas a control construct with the calcineurin-binding motif mutated displayed no such effects. Adenoviral gene transfer of dominant negative NFAT rendered cardiomyocytes resistant toward all aspects of calcineurin or agonist-induced cardiomyocyte hypertrophy, whereas adenoviral gene transfer of the control construct had no discernable effect on these parameters. These results indicate that multiple NFAT isoforms are expressed in cardiomyocytes where they function as necessary transducers of calcineurin in facilitating cardiomyocyte hypertrophy.
An open framework for multi-source, cross-domain personalisation with semantic interest graphs (2012)

Cross-domain recommendations are currently available in closed, proprietary social networking ecosystems such as Facebook, Twitter and Google+. I propose an open framework as an alternative, which enables cross-domain recommendations with domain-agnostic user profiles modeled as semantic interest graphs. This novel framework covers all parts of a recommender system. It includes an architecture for privacy-enabled profile exchange, a distributed and domain-agnostic user model and a cross-domain recommendation algorithm. This enables users to receive recommendations for a target domain (e.g. food) based on any kind of previous interests.
Objective and automated protocols for the evaluation of biomedical search engines using No Title Evaluation protocols (2008)

Our results suggest that the NT Evaluation protocols described here could be used to optimize some search engine parameters before human evaluation. Further research is needed to determine if NT Evaluation or variants of these protocols can fully substitute for human evaluations.
Evaluation of day one embryo quality and IVF outcome – a comparison of two scoring systems (2009)

The results indicate that early cleavage is a better independent marker of implantation potential than zygote morphology. The best outcome can be achieved if both embryo scoring systems are used jointly and the embryo is classified as EC and Pattern "0".
Peripheral Blood Stem Cell Mobilization by Chemotherapy With and Without Recombinant Human Granulocyte Colony-Stimulating Factor (1992)

Chemotherapy can serve as a stimulus for mobilizing hematopoietic progenitor cells to the peripheral blood for harvest via leukapheresis. Mobilized peripheral blood stem cells (PBSC) support rapid hematologic reconstitution after bone marrow aplasia induced by intensive myelosuppressive treatments. Our purpose was to develop effective mobilization regimens allowing collection of large quantities of PBSC. We administered high-dose cyclophosphamide (HDC, 4 gm/m2) or cyclophosphamide (4 gm/m2) plus etoposide (600 mg/m2) (HDCE) in a nonrandomized, sequential fashion to 94 patients with breast cancer, lymphoma, and other malignancies with collection of PBSC via leukapheresis during white blood cell (WBC) recovery from nadir counts. Each apheresis product was analyzed for total nucleated cell number, granulocyte-macrophage colony-forming units (CFU-GM) and CD34+ cells. Twenty-four additional patients with comparable pretreatment characteristics received HDCE plus recombinant human granulocyte colony-stimulating factor (HDCE+G) after chemotherapy through the end of apheresis. Patients receiving HDC were matched for age, sex, and disease but were more heavily pretreated. HDCE was superior to HDC in mean daily CFU-GM and CD34+ yield (p < 0.05), even when groups were adjusted for performance status and amount of prior therapy. HDCE+G led to 3.7 times more CFU-GM and 4.7 times more CD34+ cells than HDCE. Target PBSC yield, defined as > 20 x 10(4) CFU-GM/kg and >4 x 10(8) cells/kg, was achieved by 92% of HDCE+G patients after a median of three aphereses, 56% of HDCE patients after five aphereses, and 16% of HDC patients after six apheresis (p < 0.0001). Prior chemotherapy inversely correlated with the quantity of PBSC harvested regardless of regimen utilized. Our results demonstrate effective chemotherapy regimens for harvesting hematopoietic progenitors in a diverse patient population. HDCE+G produced the highest number of progenitors, suggesting that increasing dose intensity and adding rhG-CSF enhances mobilization. Correlation between cumulative CD34+ and CFU-GM allows real-time flow cytometric analysis of the number of aphereses required to harvest target numbers of PBSC.
The E5 Transforming Gene of Bovine Papillomavirus Encodes a Small, Hydrophobic Polypeptide (1986)

Bovine papillomavirus (BPV-1) contains two independent transforming genes that have been mapped to the E5 and E6 open reading frames (ORF's). The E5 transforming protein was identified by means of an antiserum against a synthetic peptide corresponding to the 20 COOH-terminal amino acids of the E5 ORF. The E5 polypeptide is the smallest viral transforming protein yet characterized; it had an apparent size of 7 kilodaltons. The transforming polypeptide is encoded entirely within the second half of the E5 ORF and its predicted amino acid composition is very unusual; 68% of the amino acids are strongly hydrophobic and 34% are leucine. Cell fractionation studies localized this polypeptide predominantly to cellular membranes.
Stable Green Electroluminescence from an Iridium Tris-Heteroleptic Ionic Complex (2012)

An ionic tris-heteroleptic iridium complex gives green light-emitting electrochemical cells (LECs) with unprecedented performances for this part of the visible spectrum. The devices are very bright (>1000 cd m–2), efficient (∼3%), and stable (>55 h). The novel complex is prepared using a new and efficient synthetic procedure. We show that there is a mixed orbital formation originating from the two different orthometalating ligands resulting in photophysical properties that lie between those of its two bis-heteroleptic analogs. Therefore, tris-heteroleptic complexes provide new avenues for fine-tunning the emission properties and to bridge gaps between a series of bis-heteroleptic complexes.
Nanostructuring and thermoelectric properties of semiconductor tellurides (2007)

Bulk nanostructured (Bi,Sb) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> Te <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> compounds and GeTe based amorphous/nanocrystal composites have been successfully fabricated by the combined hydrothermal/hot-pressing and quenching/annealing methods, respectively. The (Bi,Sb) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> Te <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> nanopowders synthesized by hydrothermal method exhibit a hollow-like structure. After hot-pressing, the nanoscale grains varying from tens to hundreds of nanometers were found in the bulk sample. The maximum ZT value of this (Bi,Sb) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> Te <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> sample is higher than the zone-melt one at room temperature. The sizes of the nanocrystals in the bulk GeTe nanocomposites were below 10 nm, the presence of which contributed to the remarkable improvement of the electrical conductivity and thermoelectric power factor compared to the amorphous precursors.
Induction and Characterization of a Cellobiose Dehydrogenase Produced by a Species of Monilia (1980)

A species of the imperfect fungus Monilia produced cellobiose dehydrogenase extracellularly when grown on cellulose. The inducible enzyme was both bound to the mycelium and released into the growth medium. The enzyme showed a high degree of specificity for cellobiose, but also oxidized lactose and 4-β-glucosylmannose. The specificity of the electron acceptor was restricted to those compounds having a redox potential of +0·22 V. p-Benzoquinone and several other quinones, however, were not reduced. Oxygen was not consumed nor was hydrogen peroxide produced by cellobiose dehydrogenase oxidation of cellobiose. The enzyme had a molecular weight of 48000 and an isoelectric point of 5·3 to 5·5. A new zymogram technique was developed for the detection of cellobiose dehydrogenase in polyacrylamide gels following electrophoresis and isoelectric focusing.
A New Pathway for Poly(3-hydroxybutyrate) Production inEscherichia coliandCorynebacterium glutamicumby Functional Expression of a New Acetoacetyl-coenzyme A Synthase (2011)

A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] was developed in Escherichia coli and Corynebacterium glutamicum by an acetoacetyl-coenzyme A (CoA) synthase (AACS) recently isolated from terpenoid-producing Streptomyces sp. strain CL190. Expression of AACS led to significant productions of P(3HB) in E. coli (10.5 wt %) and C. glutamicum (19.7 wt %).
Improved protein–ligand docking using GOLD (2003)

The Chemscore function was implemented as a scoring function for the protein-ligand docking program GOLD, and its performance compared to the original Goldscore function and two consensus docking protocols, "Goldscore-CS" and "Chemscore-GS," in terms of docking accuracy, prediction of binding affinities, and speed. In the "Goldscore-CS" protocol, dockings produced with the Goldscore function are scored and ranked with the Chemscore function; in the "Chemscore-GS" protocol, dockings produced with the Chemscore function are scored and ranked with the Goldscore function. Comparisons were made for a "clean" set of 224 protein-ligand complexes, and for two subsets of this set, one for which the ligands are "drug-like," the other for which they are "fragment-like." For "drug-like" and "fragment-like" ligands, the docking accuracies obtained with Chemscore and Goldscore functions are similar. For larger ligands, Goldscore gives superior results. Docking with the Chemscore function is up to three times faster than docking with the Goldscore function. Both combined docking protocols give significant improvements in docking accuracy over the use of the Goldscore or Chemscore function alone. "Goldscore-CS" gives success rates of up to 81% (top-ranked GOLD solution within 2.0 A of the experimental binding mode) for the "clean list," but at the cost of long search times. For most virtual screening applications, "Chemscore-GS" seems optimal; search settings that give docking speeds of around 0.25-1.3 min/compound have success rates of about 78% for "drug-like" compounds and 85% for "fragment-like" compounds. In terms of producing binding energy estimates, the Goldscore function appears to perform better than the Chemscore function and the two consensus protocols, particularly for faster search settings. Even at docking speeds of around 1-2 min/compound, the Goldscore function predicts binding energies with a standard deviation of approximately 10.5 kJ/mol.
The CCN family: a new stimulus package (2003)

The CCN family comprises cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). These proteins stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest and migration of multiple cell types. Many of these activities probably occur through the ability of CCN proteins to bind and activate cell surface integrins. Accumulating evidence supports a role for these factors in endocrine pathways and endocrine-related processes. To illustrate the broad role played by the CCN family in basic and clinical endocrinology, this Article highlights the relationship between CCN proteins and hormone action, skeletal growth, placental angiogenesis, IGF-binding proteins and diabetes-induced fibrosis.
Types of Hair Dye and Their Mechanisms of Action (2015)

Hair color change by dye application is a common procedure among women. Hair dyes are classified, according to color resistance, into temporary, semipermanent, demipermanent and permanent. The first two are based on molecules which are already colored. Temporary dyes act through dye deposition on cuticles, but semipermanent may penetrate a little into the cortex and so the color resists up to six washes. Demipermanent and permanent dyes are based on color precursors, called oxidation dyes, and the final shade is developed by their interactions with an oxidizing agent, but they differ from the alkalizing agent used. In oxidation systems, there is an intense diffusion of the molecules into the cortex, what promotes a longer color resistance. Dyes and color precursors present differences related to chromophore groups, hair fiber affinity, water solubility, and photo stability. The aim of this review is to discuss the differences among hair dye products available in the market and their action mechanisms, molecular structures, application methods, and some aspects of formulations.
Optogenetically induced olfactory stimulation in Drosophila larvae reveales the neuronal basis of odor-aversion behavior (2010)

Olfactory stimulation induces an odor-guided crawling behavior of Drosophila melanogaster larvae characterized by either an attractive or a repellent reaction. In order to understand the underlying processes leading to these orientations we stimulated single olfactory receptor neurons (ORNs) through photo-activation within an intact neuronal network. Using the Gal4-UAS system two light inducible proteins, the light-sensitive cation channel channelrhodopsin-2 (ChR-2) or the light-sensitive adenylyl cyclase (Pacalpha) were expressed in all or in individual ORNs of the larval olfactory system. Blue light stimulation caused an activation of these neurons, ultimately producing the illusion of an odor stimulus. Larvae were tested in a phototaxis assay for their orientation toward or away from the light source. Here we show that activation of Pacalpha expressing ORNs bearing the receptors Or33b or Or45a in blind norpA mutant larvae induces a repellent behavior away from the light. Conversely, photo-activation of the majority of ORNs induces attraction towards the light. Interestingly, in wild type larvae two ligands of Or33b and Or45a, octyl acetate and propionic ethylester, respectively, have been found to cause an escape reaction. Therefore, we combined light and odor stimulation to analyze the function of Or33b and Or45a expressing ORNs. We show that the larval olfactory system contains a designated neuronal pathway for repellent odorants and that activation of a specific class of ORNs already determines olfactory avoidance behavior.
OFDM Link Performance With Companding for PAPR Reduction in the Presence of Non-Linear Amplification (2006)

Use of companding for peak-to-average-power ratio (PAPR) control is explored for a link involving a nonlinear transmit power amplifier with orthogonal frequency division multiplexing (OFDM). Specifically, the objective of the study was to determine if companding using u-law compression/expansion at the transmitter/receiver, respectively, provides end-to-end performance gains relative to a system without companding. We consider the use of companding to ameliorate the impact of nonlinearities in the transmit amplifier. In the absence of companding, transmitter operation near saturation raises the signal level at the receiver but, because of the nonlinearities in the amplifier response, also results in distortion that impacts overall link performance. As the transmit power is backed-off from saturation, amplifier distortion is reduced, but error components due to lower SNR at the receiver become more significant. When companding is introduced in the system, the system is able to operate closer to saturation without substantial transmit distortion. However, requisite expansion of the compressed signal at the receiver yields noise amplification which can counteract any of the performance gains that would otherwise accrue from the increased SNR at the receiver. At issue is whether or not operating conditions exist (e.g., backoff, SNR, amplifier linearity model, etc) for which companding enhances the end-to-end performance relative to the link performance without companding. System simulation models were employed using Rapp's nonlinear power amplification models, where average symbol distance errors were used as performance metrics. We found that companding can provide very modest performance gains in comparison to systems that do not employ companding. Performance trends were corroborated in a hardware testbed with an amplifier chain, where average bit error rates were experimentally determined.
Findings of the First Consensus Conference on Medical Emergency Teams* (2006)

Patients needing an RRS intervention are suddenly critically ill and have a mismatch of resources to needs. Hospitals should implement an RRS, which consists of four elements: an afferent, "crisis detection" and "response triggering" mechanism; an efferent, predetermined rapid response team; a governance/administrative structure to supply and organize resources; and a mechanism to evaluate crisis antecedents and promote hospital process improvement to prevent future events.
Characterization of MAD2B and Other Mitotic Spindle Checkpoint Genes (1999)

Aneuploidy is a characteristic of the majority of human cancers, and recent work has suggested that mitotic checkpoint defects play a role in its development. To further explore this issue, we isolated a novel human gene, MAD2B (MAD2L2), which is homologous to the spindle checkpoint gene MAD2 (MAD2L1). We determined the chromosomal localization of it and other spindle checkpoint genes, including MAD1L1, MAD2, BUB3, TTK (MPS1L1), and CDC20. In addition, we resolved the genomic intron-exon structure of the human BUB1 gene. We then searched for mutations in these genes in a panel of 19 aneuploid colorectal tumors. No new mutations were identified, suggesting that genes yet to be discovered are responsible for most of the checkpoint defects observed in aneuploid cancers.