gebhart/patrepeval huggingface dataset:
Microoptic grating multiplexers and optical isolators for fiber-optic communications (1980)

As new optical devices for increasing further the utility of and to expand the application of fiber-optic communications, grating multiplexers and isolators have been developed for 0.8 μm band employing microoptic approach. The development of these devices is the subject of this paper. The devices have desirable features of small size, compactness, high optical performances, and high reliability. The grating multiplexer consists of a graded-index rod, a blazed reflection grating replicated onto the graded-index rod slanting facet or a wedge facet, and an input-output fiber array. Simple calculations have been done to determine necessary element parameters for a given channel spacing. Experimental results are presented for five-channel multiplexers devised using a SELFOC <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">®</sup> lens. Around 3 dB insertion loss and less than -30 dB crosstalk have been obtained for about 35 nm channel spacing in overall device size of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">18 \times 13 \times 50</tex> mm. Faraday rotation optical isolators for 0.8 μm band have been miniaturized by employing an efficient paramagnetic glass Faraday rotator, a magnet with a through hole and a folded optics in the Faraday rotator. The path number in the folded optics has been optimized in terms of trading-off between the magnet size and the insertion loss. A 0.9 dB insertion loss including fiber coupling loss and 36 dB isolation have been obtained in overall device size of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">24 \times 24.5 \times 42</tex> mm. Results on the temperature and wavelength dependence of the isolation are also presented. In addition, fundamental properties of optical circulators for 0.8 μm band and optical isolators and circulators both for 1.3 μm band, developed as extended modifications of the optical isolators for 0.8 μm band, are briefly described.
Knowledge, attitudes, and screening practices among older men regarding prostate cancer (2000)

Many New York men appear to be unaware of risk factors for prostate cancer. However, a substantial percentage reported having been screened for the disease; physician advice may have been a major determining factor in their decision to be tested.
Effects of aloesin on melanogenesis in pigmented skin equivalents (2008)

Reconstituted 3-dimensional human skin equivalents in vitro are gaining popularity for studies of skin metabolism and depigmenting agents because they exhibit morphological and growth characteristics similar to human skin. The effects of aloesin on melanogenesis, however, have never been examined with the pigmented skin equivalent. The purpose of the study was to construct the skin equivalent and observe the effects of aloesin on melanogenesis in the model. We constructed an in vitro pigmented skin equivalent and examined the general structure and condition of the pigmented skin equivalent with H&E staining and Fontana Masson staining. Then, we examined the effects of aloesin on tyrosinase activity and formation of melanin in the model. Such a pigmented skin model demonstrated morphology similar to that seen in normal skin and can be used to assess the regulation of pigmentation by melanogenic compounds. The results suggested that aloesin had direct inhibitory effects on melanogenesis and showed dose-dependent reductions in tyrosinase activity (P < 0.05) and melanin content (P < 0.05). In conclusion, our study indicated that skin equivalents provided a convenient and cost-effective alternative to animal testing for evaluating the regulation of pigmentation. Aloesin showed promise as a pigmentation-altering agent for cosmetic or therapeutic applications.
Characterization of a G-protein Activator in the Neuroblastoma-Glioma Cell Hybrid NG108-15 (1996)

Purified bovine brain G-protein was used in a solution phase assay to identify membrane-associated proteins that influenced the activation of heterotrimeric G-proteins. Detergent-solubilized membrane extracts from the neuroblastoma-glioma cell hybrid NG108-15, but not the parent C6B4 glioma cell line, increased [35S]GTPγS binding to purified G-protein by ∼460%. The G-protein activator was heat-sensitive, and the magnitude of its action was related to the amount of extract protein. The biophysical and biochemical properties of the G-protein activator were determined using DEAE ion exchange chromatography, gel filtration, and a lectin affinity matrix. In the presence of added GDP (1 μM), the enriched G-protein activator increased the initial rate of [35S]GTPγS binding to brain G-protein by up to 4-fold. In the absence of added GDP, the G-protein activator elicited an initial burst in [35S]GTPγS binding to brain G-protein within the first 30 s, after which the rate of nucleotide binding to G-protein was similar in the absence or presence of the G-protein activator. The stimulation of nucleotide binding to brain G-protein by the activator was also observed after resolution of Gα from Gβγ. The G-protein activator was distinct from other proteins (neuromodulin, tubulin, and β-amyloid precursor protein) that influence nucleotide binding to G-protein, indicating the existence of a novel signal accelerator. Purified bovine brain G-protein was used in a solution phase assay to identify membrane-associated proteins that influenced the activation of heterotrimeric G-proteins. Detergent-solubilized membrane extracts from the neuroblastoma-glioma cell hybrid NG108-15, but not the parent C6B4 glioma cell line, increased [35S]GTPγS binding to purified G-protein by ∼460%. The G-protein activator was heat-sensitive, and the magnitude of its action was related to the amount of extract protein. The biophysical and biochemical properties of the G-protein activator were determined using DEAE ion exchange chromatography, gel filtration, and a lectin affinity matrix. In the presence of added GDP (1 μM), the enriched G-protein activator increased the initial rate of [35S]GTPγS binding to brain G-protein by up to 4-fold. In the absence of added GDP, the G-protein activator elicited an initial burst in [35S]GTPγS binding to brain G-protein within the first 30 s, after which the rate of nucleotide binding to G-protein was similar in the absence or presence of the G-protein activator. The stimulation of nucleotide binding to brain G-protein by the activator was also observed after resolution of Gα from Gβγ. The G-protein activator was distinct from other proteins (neuromodulin, tubulin, and β-amyloid precursor protein) that influence nucleotide binding to G-protein, indicating the existence of a novel signal accelerator.
Dose escalation, safety and immunogenicity study of a tetravalent meninogococcal polysaccharide diphtheria conjugate vaccine in toddlers (2002)

Two injections of tetravalent (Groups A, C, Y and W-135) meningococcal polysaccharide vaccine conjugated to diphtheria were given to 30 toddlers at dosages of 1, 4 and 10 microg/ml polysaccharide of each serogroup. Reactogenicity was acceptable at all dosages. The 4-microg/ml dose appears to be immunologically optimal.
An evolutionary conserved pattern of 18S rRNA sequence complementarity to mRNA 5′ UTRs and its implications for eukaryotic gene translation regulation (2013)

There are several key mechanisms regulating eukaryotic gene expression at the level of protein synthesis. Interestingly, the least explored mechanisms of translational control are those that involve the translating ribosome per se, mediated for example via predicted interactions between the ribosomal RNAs (rRNAs) and mRNAs. Here, we took advantage of robustly growing large-scale data sets of mRNA sequences for numerous organisms, solved ribosomal structures and computational power to computationally explore the mRNA-rRNA complementarity that is statistically significant across the species. Our predictions reveal highly specific sequence complementarity of 18S rRNA sequences with mRNA 5' untranslated regions (UTRs) forming a well-defined 3D pattern on the rRNA sequence of the 40S subunit. Broader evolutionary conservation of this pattern may imply that 5' UTRs of eukaryotic mRNAs, which have already emerged from the mRNA-binding channel, may contact several complementary spots on 18S rRNA situated near the exit of the mRNA binding channel and on the middle-to-lower body of the solvent-exposed 40S ribosome including its left foot. We discuss physiological significance of this structurally conserved pattern and, in the context of previously published experimental results, propose that it modulates scanning of the 40S subunit through 5' UTRs of mRNAs.
The Research of External Financing Options of Small and Medium-Sized Enterprises in China (2012)

Since the reform and opening up, small and medium-sized enterprises (SMEs) in China developed rapidly, which have become an important part of China’s economic and social development. However, the financing difficulties of SMEs have become increasingly prominent during recent years, and it hinders the healthy development of SMEs in China. This paper analyzes the current financing situation of SMEs in China, and conducts a deeply comparative analysis for several common external financing means of SMEs. In addition, this article provides some suggestions about how to solve financing difficulties if SMEs in China. Key words: Small and medium-sized enterprises (SMEs); External financing means; Options
Experimental study of turbulent flows through pipe bends (2012)

This thesis deals with turbulent flows in 90 degree curved pipes of circular cross-section. The flow cases investigated experimentally are turbulent flow with and without an additional motion, swirling or pulsating, superposed on the primary flow. The aim is to investigate these complex flows in detail both in terms of statistical quantities as well as vortical structures that are apparent when curvature is present. Such a flow field can contain strong secondary flow in a plane normal to the main flow direction as well as reverse flow. The motivation of the study has mainly been the presence of highly pulsating turbulent flow through complex geometries, including sharp bends, in the gas exchange system of Internal Combustion Engines (ICE). On the other hand, the industrial relevance and importance of the other type of flows were not underestimated. The geometry used was curved pipes of different curvature ratios, mounted at the exit of straight pipe sections which constituted the inflow conditions. Two experimental set ups have been used. In the first one, fully developed turbulent flow with a well defined inflow condition was fed into the pipe bend. A swirling motion could be applied in order to study the interaction between the swirl and the secondary flow induced by the bend itself. In the second set up a highly pulsating flow (up to 40 Hz) was achieved by rotating a valve located at a short distance upstream from the measurement site. In this case engine-like conditions were examined, where the turbulent flow into the bend is non-developed and the pipe bend is sharp. In addition to flow measurements, the effect of non-ideal flow conditions on the performance of a turbocharger was investigated. Three different experimental techniques were employed to study the flow field. Time-resolved stereoscopic particle image velocimetry was used in order to visualize but also quantify the secondary motions at different downstream stations from the pipe bend while combined hot-/cold-wire anemometry was used for statistical analysis. Laser Doppler velocimetry was mainly employed for validation of the aforementioned experimental methods. The three-dimensional flow field depicting varying vortical patterns has been captured under turbulent steady, swirling and pulsating flow conditions, for parameter values for which experimental evidence has been missing in literature.
MeCP2 Dysfunction in Humans and Mice (2005)

Rett syndrome is a leading cause of postnatal neurodevelopmental regression. Rett syndrome is caused by mutations in MECP2, the gene encoding methyl-CpG binding protein 2. In up to 96% of all classic cases, Rett syndrome cases are caused by mutations or deletions in MECP2. The phenotypic spectrum of MECP2 mutations is broad and includes mental retardation with or without seizures, Angelman syndrome-like phenotype, and autism. Mecp308/Y mice carry a truncating mutation and display many of the features seen in Rett syndrome. Social behavior abnormalities and impaired social interactions in Mecp308/Y mice suggest that MeCP2 plays a role in modulating the activity of genes and neurons important for social interactions. Mice that overexpress MeCP2 at twice the endogenous levels develop a progressive neurologic disorder, demonstrating that MeCP2 levels are tightly regulated and raising the possibility that duplications or gain-of-function mutations of MECP2 might underlie some cases of neurodevelopmental X-linked disorders.
Identification of DFNA5 as a target of epigenetic inactivation in gastric cancer (2006)

Epigenetic gene inactivation plays a key role in the development of various types of cancer. Using methylated CpG island amplification coupled with representational difference analysis to identify genes inactivated by DNA methylation in gastric cancer, we identified seven DNA fragments corresponding to the 5' CpG islands of the affected genes. One of the clones recovered was identical to the 5' flanking region of DFNA5, a gene previously shown to be associated with deafness and induced by DNA damage. Further analysis revealed that DFNA5 is expressed in normal tissues but is down-regulated in gastric cancer cell lines due to methylation of the region around its transcription start site. Treating gastric cancer cells that lacked DFNA5 expression with a methyltransferase inhibitor, 5-aza-2'-deoxycytidine, restored the gene's expression. Methylation of DFNA5 was detected in 50% of primary gastric tumors, and was correlated with positivity for Epstein-Barr virus and the absence of metastasis. Moreover, introduction of exogenous DFNA5 into silenced cells suppressed colony formation. Taken together, these data suggest that the silencing of DFNA5 occurs frequently in gastric cancer and may play a key role in development and progression of the disease.
Detection of an unusually stable fibrinolytic inhibitor produced by bovine endothelial cells. (1983)

Fibrin/agar films were prepared and used to detect plasminogen activators produced by cultured bovine aortic endothelial cells (fibrin autography). One preparation of fibrin underwent spontaneous lysis upon incubation at 37 degrees C. This lysis was prevented by antibodies to tissue-type plasminogen activator but not by antibodies to urokinase. Conditioned medium from the confluent endothelial cells was fractionated by polyacrylamide gel electrophoresis in the presence of NaDodSO4. The gels were analyzed on indicator films prepared with the spontaneously lysing fibrin (reverse fibrin autography). Unexpectedly, as the opaque fibrin film cleared, a distinct lysis-resistant zone appeared in the indicator gel at a region corresponding to Mr 55,000. Experiments were devised to determine whether the lysis-resistant zone in the indicator film reflected the presence of a cellular inhibitor in the polyacrylamide gel. The corresponding region was excised from a polyacrylamide gel, extracted with buffer, and tested directly for antifibrinolytic activity by the 125I-labeled fibrin plate method. Urokinase-mediated fibrinolytic activity was inhibited by the gel extract in a dose-dependent manner indicating the presence of such an inhibitor. Inhibitor activity was detected in Triton X-100 extracts of washed monolayers and in conditioned medium, where it accumulated with time. The endothelial cell inhibitor not only survived exposure to NaDodSO4 but also was active after incubation at pH 12 or treatment with 5% (vol/vol) 2-mercaptoethanol, 6 M urea, 4 M guanidine hydrochloride, or 1 M acetic acid. Considerable activity also remained after heating at 100 degrees C for 30 min. These results indicate that cultured bovine aortic endothelial cells synthesize and secrete a previously undetected, unusually stable fibrinolytic inhibitor of Mr 55,000. Reverse fibrin autography offers a convenient approach for studying such molecules.
Expression of Concern: Neurotensin and EGF induce synergistic stimulation of DNA synthesis by increasing the duration of ERK signaling in ductal pancreatic cancer cells (2004)

Neurotensin (NT) and epidermal growth factor (EGF) induced rapid extracellular-regulated protein kinase (ERK) activation through different signaling pathways in the K-Ras mutated human pancreatic carcinoma cell lines PANC-1 and MIA PaCa-2. NT stimulated ERK activation via a protein kinase C (PKC)-dependent (but EGF receptor-independent) pathway in PANC-1 and MIA PaCa-2 cells, whereas EGF promoted ERK activation through a PKC-independent pathway in these cells. Concomitant stimulation of these cells with NT and EGF induced a striking increase in the duration of ERK pathway activation as compared with that obtained in cells treated with each agonist alone. Stimulation with NT + EGF promoted synergistic stimulation of DNA synthesis and anchorage-independent growth. Addition of the MEK inhibitor U0126, either prior to stimulation with NT + EGF or 2 h after stimulation with NT + EGF prevented the synergistic increase in DNA synthesis and suppressed the sustained phase of ERK activation. Furthermore, treatment with the selective PKC inhibitor GF-1 converted the sustained ERK activation in response to NT and EGF into a transient signal and also abrogated the synergistic increase in DNA synthesis. Collectively, our results suggest that the sustained phase of ERK signaling mediates the synergistic effects of NT and EGF on DNA synthesis in pancreatic cancer cells.
NF-Y–Dependent Cyclin B2 Expression in Colorectal Adenocarcinoma (2007)

Cyclin B2 seems to be a molecular marker of a colorectal adenocarcinoma and that its up-regulation and coordinate expression of the other cell cycle-related genes by NF-Y might contribute to tumor cell proliferation by accelerating cell cycle progression.
Differential pattern of T cell recognition of the 65‐kDa mycobacterial antigen following immunization with the whole protein or peptides (1989)

The 65-kDa stress protein from Mycobacterium bovis (Bacillus Calmette Guérin) elicited T cell proliferation and antibody responses in seven B10 congenic mouse strains with different H-2 haplotypes. To analyze T cell determinants on this antigen, seven peptides corresponding to six predicted T cell epitopes, and one defined B cell epitope were synthesized. Mice were either immunized with the whole antigen and the specificity of the response was ascertained in respect of the six peptides, or mice were immunized with seven of the peptides and tested for proliferative responses to the whole molecule. The results showed that three peptides carried epitopes to which mice responded following injection of the whole molecule and that immunization with two additional peptides could prime for in vitro stimulation with the native antigen. The latter result indicates the feasibility of generating T cell responses to "cryptic" epitopes on proteins by immunizing with peptides. The peptide-specific T cell responses were distinctly influenced by the H-2 haplotype of mouse strains. However, two peptides were recognized by several H-2-disparate mouse strains, and one peptide could be presented by both I-A and I-E molecules. Immunization with several peptides induced a cross-reactive T cell proliferative response to the homologous GroEL protein isolated from E. coli. The amount of cross-reactivity was influenced by the extent of sequence homology between mycobacterial and E. coli proteins and the major histocompatibility complex class II molecule used to present the peptide.
Transcriptional regulation of gene expression: Mechanisms and pathophysiology (1994)

Transcription factors are key mediators of the genetic programs that underlie human development and physiology. Mutations in genes that encode transcription factors or in DNA sequences to which these factors bind may adversely affect gene expression and result in disease. Mutations in genes encoding transcription factors often have pleiotropic effects because each transcription factor is involved in the regulation of multiple genes. For several transcription factors, germline mutations have been shown to result in malformation syndromes whereas somatic mutations in the same genes contribute to the multistep process of tumorigenesis. The study of transcription factors and their involvement in human disease thus provides insight into the molecular mechanisms underlying human development, physiology, dysmorphology, and oncology.
Energy from algae using microbial fuel cells (2009)

Bioelectricity production from a phytoplankton, Chlorella vulgaris, and a macrophyte, Ulva lactuca was examined in single chamber microbial fuel cells (MFCs). MFCs were fed with the two algae (as powders), obtaining differences in energy recovery, degradation efficiency, and power densities. C. vulgaris produced more energy generation per substrate mass (2.5 kWh/kg), but U. lactuca was degraded more completely over a batch cycle (73 +/- 1% COD). Maximum power densities obtained using either single cycle or multiple cycle methods were 0.98 W/m(2) (277 W/m(3)) using C. vulgaris, and 0.76 W/m(2) (215 W/m(3)) using U. lactuca. Polarization curves obtained using a common method of linear sweep voltammetry (LSV) overestimated maximum power densities at a scan rate of 1 mV/s. At 0.1 mV/s, however, the LSV polarization data was in better agreement with single- and multiple-cycle polarization curves. The fingerprints of microbial communities developed in reactors had only 11% similarity to inocula and clustered according to the type of bioprocess used. These results demonstrate that algae can in principle, be used as a renewable source of electricity production in MFCs.
Inhibition of DNA helicase II unwinding and ATPase activities by DNA-interacting ligands : kinetics and specificity (2021)

Although DNA helicases play important roles in the processing of DNA, little is known about the effects of DNA-interacting ligands on these helicases. Therefore, the effects of a wide variety of DNA-binding ligands on the unwinding and ATPase reactions catalyzed by Escherichia coli DMA helicase II were examined. DNA minor groove binders and simple DNA intercalators did not inhibit helicase II. However, DNA intercalators, such as mitoxantrone and nogalamycin, which position functionalities in the major groove upon binding duplex DNA, were potent inhibitors of helicase II.Although DNA helicases play important roles in the processing of DNA, little is known about the effects of DNA-interacting ligands on these helicases. Therefore, the effects of a wide variety of DNA-binding ligands on the unwinding and ATPase reactions catalyzed by Escherichia coli DMA helicase II were examined. DNA minor groove binders and simple DNA intercalators did not inhibit helicase II. However, DNA intercalators, such as mitoxantrone and nogalamycin, which position functionalities in the major groove upon binding duplex DNA, were potent inhibitors of helicase II.