gebhart/patrepeval huggingface dataset:
Antimicrobial Susceptibility ofArcanobacterium haemolyticum (1994)

(OrionPharmaceuticals, Espoo, Finland), cephalexin (Orion), cefuroxime (GlaxoOperations, Ltd., Greenford, England), cefotaxime (Hoechst AG, Frankfurt, Germany), erythromycin (Orion), azithromycin (Pfizer GmbH,Karlsruhe, Germany), doxycycline (Orion), ciprofloxacin (Bayer, Leverkusen, Germany), clindamycin (Upjohn Co.,Kalamazoo, Mich.), vancomycin (Eli Lilly and Co.,Indianapolis, Ind.), andtrimethoprim-sulfamethoxazole (Orion). TheMICswere determined bytheagardilution method(7) on Mueller-Hinton IIagar(BBL,Cockeysville, Md.)supplementedwith5%defibrinated sheep bloodand1%IsoVitalex (BBL).Bacteria harvested fromsheepbloodagarplates after48h ofincubation at35'Cina candlejarwere suspended in0.9%saline andadjusted to a density of approximately 0.5on theMcFarland turbidity standard. The suspension was further diluted 1 in10insaline. Witha multipoint inoculator (MastLaboratories Ltd., Bootles, England), a final inoculum ofapproximately 104CFU was delivered ontoMueller-Hinton plates. TheMueller-Hinton plates were incubated overnight at35°Cinambient air. The MIC was determined as thelowestconcentration ofthe antimicrobial agentthatinhibited macroscopic growth. The following control organisms were inoculated on eachplate: Escherichia coliATCC 25922, Staphylococcus aureus ATCC29213andA.haemolyticum ATCC9345.
Efficiency and precision of electroimmunoassay for canine factor VIII-related antigen. (1983)

Factors that improved the efficiency, precision, and sensitivity of the electroimmunoassay for canine factor VIII-related antigen were investigated. These included rapid electrophoresis, increased rocket heights, deletion of the gel-washing step, and doubling of the sample capacity per plate. The modified assay was reliable and accurate, could be completed within 3 hours, and permitted twice as many determinations. Other experimental variables that affected the assay were the type of agarose and sample diluent, storage conditions of samples before assay, source of antibody, and use of 2 mM calcium lactate.
Influence of probenecid (PR) and cimetidine (C) on pramipexole (PX) pharmacokinetics. (1996)

Clinical Pharmacology & Therapeutics (1996) 59, 183–183; doi: 10.1038/sj.clpt.1996.233
Process intensification of biodiesel production using a continuous oscillatory flow reactor (2003)

Abstract Oscillatory flow reactors (OFRs) are a novel type of continuous reactor, consisting of tubes containing equally spaced orifice plate baffles. An oscillatory motion is superimposed upon the net flow of the process fluid, creating flow patterns conducive to efficient heat and mass transfer, whilst maintaining plug flow. Unlike conventional plug flow reactors, where a minimum Reynolds number must be maintained, the degree of mixing is independent of the net flow, allowing long residence times to be achieved in a reactor of greatly reduced length‐to‐diameter ratio. Many long residence time processes are currently performed in batch, as conventional designs of plug flow reactor prove to be impractical due to their high length‐to‐diameter ratios, which lead to problems such as high capital cost, large ‘footprint’, high pumping costs and, also control is difficult. The OFR allows these processes to be converted to continuous, thereby intensifying the process. The transesterification of various natural oils to form ‘biodiesel’ is a ‘long’ reaction, usually performed in batch. Conversion to continuous processing should improve the economics of the process, as the improved mixing should generate a better product (rendering the downstream separation processes easier), at lower residence time (reduction in reactor volume). These improvements can decrease the price of ‘biodiesel’, making it a more realistic competitor to ‘petrodiesel’. This paper shows that it is feasible to perform this reaction in an OFR at a lower residence time. The reaction was performed in a pilot‐scale plant, using rapeseed oil and methanol as the feedstocks, and NaOH as the catalyst. © 2003 Society of Chemical Industry
Sialobiology of Influenza: Molecular Mechanism of Host Range Variation of Influenza Viruses (2005)

The gene pool of influenza A viruses in aquatic birds provides all of the genetic diversity required for human and lower animals. Host range selection of the receptor binding specificity of the influenza virus hemagglutinin occurs during maintenance of the virus in different host cells that express different receptor sialo-sugar chains. In this paper, functional roles of the hemagglutinin and neuraminidase spikes of influenza viruses are described in the relation to 1) host range of influenza viruses, 2) receptor binding specificity of human and other animal influenza viruses, 3) recognition of sialyl sugar chains by Spanish influenza virus hemagglutinin, 4) highly pathogenic and potentially pandemic H5N1, H9N2, and H7N7 avian influenza viruses and molecular mechanism of host range variation of influenza viruses, 5) role of the neuraminidase spike for the host range of influenza viruses, and 6) Development of anti-influenza drugs.
Plasmid vectors for the rapid isolation and transcriptional analysis of human beta-globin gene alleles. (1983)

We describe the construction and characterization of miniplasmid vectors that can be used to isolate and express normal and mutant alleles of the human beta-globin gene. These vectors, designated pi SV beta plasmids, contain a bacterial origin of replication and selectable marker, a 5'-flanking beta-globin DNA fragment that can be used for recombination screening (Seed, 1983), and simian virus 40 (SV40) sequences that allow accurate and efficient expression of the beta-globin gene transfected into mammalian cells. We show that pi SV beta plasmids can be used to select cloned beta-globin genes from a bacteriophage lambda library of genomic DNA, and that plasmids containing the beta-globin gene linked to the SV40 enhancer sequence can be excised from the phage, circularized and recovered by transformation of Escherichia coli. Analysis of the beta-globin transcripts produced by the recovered pi SV beta recombinant plasmids after transfection into COS cells and replication to high copy number, indicates that the beta-globin gene is accurately transcribed, but a substantial fraction of the transcripts are the result of readthrough from sites within the vector. In contrast, when these plasmids are transferred into HeLa cells beta-globin RNA is accurately initiated and little readthrough transcription is observed. These results indicate that HeLa cells are more suitable than COS cells for studying mutant beta-globin genes, even though the copy number of the pi SV beta plasmids is much higher in COS cells.
Targeted hypoglossal neurostimulation for obstructive sleep apnoea: a 1-year pilot study (2012)

Continuous positive airway pressure (CPAP) is an effective but cumbersome treatment for obstructive sleep apnoea (OSA). Noncompliant patients need alternative therapies. We studied a tongue neurostimulation approach: targeted hypoglossal neurostimulation (THN) therapy with the aura6000™ System. A multi-contact electrode positioned around the main trunk of the twelfth nerve connected to an implanted pulse generator stimulates segments of the nerve, activating dilator muscles. The primary objective was to improve the polysomnographically determined apnoea/hypopnoea index (AHI) at 3 months, and maintain the improvement after 12 months of treatment. 13 out of 14 operated patients were successfully implanted. At 12 months, the AHI decreased from 45±18 to 21±17, a 53% reduction (p<0.001). The 4% oxygen desaturation index fell from 29±20 to 15±16 and the arousal index from 37±13 to 25±14, both p<0.001. The Epworth sleepiness scale decreased from 11±7 to 8±4 (p=0.09). THN was neither painful nor awakened patients, who all complied with therapy. There were two transient tongue paresis. The present study represents the longest study of any hypoglossal neurostimulation reported to date. We conclude that THN is safe and effective to treat OSA in patients not compliant with CPAP.
Research on forced oscillations disturbance source locating through an energy approach (2015)

Low-frequency oscillations (LFOs) are inherent phenomena of modern interconnected electrical power systems, essentially caused by the continuous exchange of momentum among rotating masses. It is widely recognized that LFOs can be induced by both the occurrence of multiple disturbances and the existence of periodic disturbance sources. LFOs can be classified into negative damped oscillations and forced power oscillations. The former can be suppressed by standard controllers (e.g., power system stabilizers). The latter cannot be eliminated until the disturbance source is removed or isolated. Thus, locating the forced disturbance source promptly and accurately is crucial. In this paper, a traditional energy function, simplified and improved, with a new decomposition way to show the direction of energy flow is proposed. Energy associated with the LFO is decomposed into oscillating component and quasi-steady component at the aim of analyzing the inducing multiple disturbances. The branch energy is hence decomposed into periodic and aperiodic components. By identifying the direction along which the aperiodic component is propagated and dissipated, the periodic forced disturbance source could be located rapidly and accurately. The proposed energy decomposition method provides a novel idea to judge the direction along which the dissipated energy flows. The effectiveness and feasibility of the proposed method were verified by simulation results obtained on an actual large-scale interconnected power grid in China. Copyright © 2015 John Wiley & Sons, Ltd.
Plaque-associated expression of human herpesvirus 6 in multiple sclerosis. (1995)

Representational difference analysis was used to search for pathogens in multiple sclerosis brains. We detected a 341-nucleotide fragment that was 99.4% identical to the major DNA binding protein gene of human herpesvirus 6 (HHV-6). Examination of 86 brain specimens by PCR demonstrated that HHV-6 was present in > 70% of MS cases and controls and is thus a commensal virus of the human brain. By DNA sequencing, 36/37 viruses from MS cases and controls were typed as HHV-6 variant B group 2. Other herpesviruses, retroviruses, and measles virus were detected infrequently or not at all. HHV-6 expression was examined by immunocytochemistry with monoclonal antibodies against HHV-6 virion protein 101K and DNA binding protein p41. Nuclear staining of oligodendrocytes was observed in MS cases but not in controls, and in MS cases it was observed around plaques more frequently than in uninvolved white matter. MS cases showed prominent cytoplasmic staining of neurons in gray matter adjacent to plaques, although neurons expressing HHV-6 were also found in certain controls. Since destruction of oligodendrocytes is a hallmark of MS, these studies suggest an association of HHV-6 with the etiology or pathogenesis of MS.
The migraine attack (2008)

Abstract This chapter covers the epidemiology of migraine, making the diagnosis of migraine, co-morbidity and associations, the migraine attack, migraine variants and complications, and when to investigate.
Insights into the Evolution of Allosteric Properties. The NADH Binding Site of Hexameric Type II Citrate Synthases, (2003)

Study of the hexameric and allosterically regulated citrate synthases (type II CS) provides a rare opportunity to gain not only an understanding of a novel allosteric mechanism but also insight into how such properties can evolve from an unregulated structural platform (the dimeric type I CS). To address both of these issues, we have determined the structure of the complex of NADH (a negative allosteric effector) with the F383A variant of type II Escherichia coli CS. This variant was chosen because its kinetics indicate it is primarily in the T or inactive allosteric conformation, the state that strongly binds to NADH. Our structural analyses show that the six NADH binding sites in the hexameric CS complex are located at the interfaces between dimer units such that most of each site is formed by one subunit, but a number of key residues are drawn from the adjacent dimer. This arrangement of interactions serves to explain why NADH allosteric regulation is a feature only of hexameric type II CS. Surprisingly, in both the wild-type enzyme and the NADH complex, the two subunits of each dimer within the hexameric conformation are similar but not identical in structure, and therefore, while the general characteristics of NADH binding interactions are similar in each subunit, the details of these are somewhat different between subunits. Detailed examination of the observed NADH binding sites indicates that both direct charged interactions and the overall cationic nature of the sites are likely responsible for the ability of these sites to discriminate between NADH and NAD(+). A particularly novel characteristic of the complex is the horseshoe conformation assumed by NADH, which is strikingly different from the extended conformation found in its complexes with most proteins. Sequence homology studies suggest that this approach to binding NADH may arise out of the evolutionary need to add an allosteric regulatory function to the base CS structure. Comparisons of the amino acid sequences of known type II CS enzymes, from different Gram-negative bacteria taxonomic groups, show that the NADH-binding residues identified in our structure are strongly conserved, while hexameric CS molecules that are insensitive to NADH have undergone key changes in the sequence of this part of the protein.
Revisiting regulation of potassium homeostasis in Escherichia coli: the connection to phosphate limitation (2017)

Two-component signal transduction constitutes the predominant strategy used by bacteria to adapt to fluctuating environments. The KdpD/KdpE system is one of the most widespread, and is crucial for K<sup>+</sup> homeostasis. In Escherichia coli, the histidine kinase KdpD senses K<sup>+</sup> availability, whereas the response regulator KdpE activates synthesis of the high-affinity K<sup>+</sup> uptake system KdpFABC. Here we show that, in the absence of KdpD, kdpFABC expression can be activated via phosphorylation of KdpE by the histidine kinase PhoR. PhoR and its cognate response regulator PhoB comprise a phosphate-responsive two-component system, which senses phosphate limitation indirectly through the phosphate transporter PstCAB and its accessory protein PhoU. In vivo two-hybrid interaction studies based on the bacterial adenylate cyclase reveal pairwise interactions between KdpD, PhoR, and PhoU. Finally, we demonstrate that cross-regulation between the kdpFABC and pstSCAB operons occurs in both directions under simultaneous K<sup>+</sup> and phosphate limitation, both in vitro and in vivo. This study for the first time demonstrates direct coupling between intracellular K<sup>+</sup> and phosphate homeostasis and provides a mechanism for fine-tuning of the balance between positively and negatively charged ions in the bacterial cell.
Structural and Mechanical Properties of Woven Fabrics Employing Peirce's Model (2003)

Peirce's model is newly studied to predict the structural and mechanical properties of woven fabrics. The study concerns various fabric structures made from different yarns of cotton, wool, and polyester, and weave structures of plain, twill, satin, and mat. Weave structures and fabric relaxation cause changes in the yam diameter and fabric crimp. Therefore, the diameters of warp and weft yams in Peirce's model are newly defined to determine effective yarn diameters. When the modified diameters are used to predict the structural and mechanical properties of the fabric, the predicted values show excellent agreement with the measured ones. The more relaxed the fabrics are, the more applicable is Peirce's model to predicting fabric thickness and tensile behavior.
ATP and calcium modulation of nonselective cation channels in IMCD cells (1994)

The mIMCD-3 cell line, developed from simian virus 40 transformed mice, was grown on coverslips for single-channel analysis of the apical membrane. An amiloride-sensitive nonselective cation channel (NCATP) was demonstrated that occurred predominantly in excised patches. The selectivity sequence for NCATP was NH4+ = Na+ = K+ = Li+ = Rb+ > Cl-. The single-channel conductance was 24 pS and nonrectifying in 140 mM KCl or NaCl solutions. NCATP was not permeable to barium from the extracellular side. NCATP was not voltage gated but was activated spontaneously upon patch excision or after applying negative pressure (20-40 mmHg) to an excised patch in bath solutions containing 1 microM Ca2+ [mean number of open channels (NPl) = 1.45]. NCATP was not activated when excised into a bath solution in which Ca2+ was reduced to 100 nM. The open probability of NCATP was reduced by 68% when 2 mM ATP was added to the intracellular side of an excised patch but was unaffected when 0.1 mM 8-bromoguanosine 3',5'-cyclic monophosphate was added to the intracellular side. In cell-attached patches, NCATP was activated upon response to a hyposmotic (210 mosmol/kgH2O) bathing solution containing 0.5 mM Ca2+ (NPo = 0.35). These results show that the mIMCD-3 cell line contains a volume-sensitive nonselective cation channel that is modulated by ATP and calcium but not guanosine 3',5'-cyclic monophosphate. It is postulated that NCATP may act to initiate the volume regulatory response in IMCD cells.
Phytase from Aspergillus terreus (1968)

1) Aspergillus terreus No. 9A-1 was cultivated by a shaking method and the optimal cultural conditions for the phytase production were concluded as follows: Composition of medium; rice bran 30g, ammonium sulfate 3g, distilled water 1.0 liter; initial pH 5.5; shaking condition; 50ml of medium/500ml vol. flask; 120 oscil./min, 90hr. 2) Phytase from Asp. terreus was purified by ammonium sulfate precipitation, acetone precipitation and chromatography on SE-Sephadex C-50 and Sephadex G-200 columns. The enzyme was purified about 520-folds with the yield of 20% from the broth. The purified enzyme was homogeneous by column chromatography, ultracentrifugation and electrophoresis. 3) This purified preparation of phytase showed following properties. a) Optimal pH for the reaction was 4.5; b) optimal temperature for the reaction was about 70°C; c) the enzyme was stable in the range of pH from 1.2 to 9.0
Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? (2004)

The limited throughput, scalability and high cost of protein purification by chromatography provide motivation for the development of non-chromatographic protein purification technologies that are cheaper and easier to implement in a high-throughput format for proteomics applications and to scale up for industrial bioprocessing. We have shown that genetic fusion of a recombinant protein to an elastin-like polypeptide (ELP) imparts the environmentally sensitive solubility property of the ELP to the fusion protein, and thereby allows selective separation of the fusion protein from Escherichia coli lysate by aggregation above a critical temperature (T(t)). Further development of ELP fusion proteins as widely applicable purification tools necessitates a quantitative understanding of how fused proteins perturb the ELP T(t) such that purification conditions (T(t)) may be predicted a priori for new recombinant proteins. We report here the effect that fusing six different proteins has on the T(t) of an ELP. A negative correlation between T(t) and the fraction hydrophobic surface area on the fused proteins was observed, which was determined from computer modeling of the available three-dimensional structure. The thermally triggered aggregation behavior of ELP-coated, functionalized gold colloids as well as ligand binding to the tendamistat-ELP fusion protein support the hypothesis that hydrophobic surfaces in molecular proximity to ELPs depress the ELP T(t) by a mechanism analogous to hydrophobic residue substitution in the ELP repeat, Val-Pro-Gly-Xaa-Gly.
Double Diels−Alder Strategies to Soluble 2,9- and 2,9,6,13-Tetraethynylpentacenes, Photolytic [4 + 4] Cycloadditions, and Pentacene Crystal Packing (2007)

Four new classes of organic solvent soluble ethynylpentacene derivatives (2,9-, 2,10-, 2,6,9,13-, 2,6,10,13-) have been prepared by complementary, versatile, double Diels-Alder strategies. Functional groups on the A, C, and E rings can be manipulated to increase the solubility, modulate the electronics, and alter the solid-state packing. Cycloaddition reactions with diene 2 and 1,4,5,8-anthradiquinone (3) or ortho-quinodimethane 19 with 1-butyl-3-methylimidazolim iodide (18) as the iodide source (a significant improvement over NaI) and benzoquinone (20) followed by in situ aromatization afforded the quinones 4, 5, 21, and 22, respectively. For the 2,9- and 2,10- families, a one-pot desilylation/triflation was developed. Palladium(0) coupling and reductive aromatization afforded 2,9-di(triisopropylsilylethynyl)pentacene (10) and 2,10-di(triisopropylsilylethynyl)pentacene (11), respectively. Photodimerization of these pentacenes afforded the air-stable [4 + 4] cycloaddition pentacene precursors (tetrakisnaphthotricyclo[4.2.2.22,5]dodecanes, 12-15). Thermal cycloreversion of the dimers ( approximately 13 J/g, approximately 4 kcal/mol) produces the parent pentacenes (10 or 11). The tetrasubstituted family utilized a parallel route with extra versatility as the timing of the Grignard and palladium(0) coupling step may be varied depending upon the functional group combinations desired. The subsequent reactions provided the tetraethynylpentacenes 28-30, 33-35 (para-isomers), and 38 (meta-isomer). X-ray crystallography analysis of 28, 29, and 33 revealed various pi-pi stacked packing motifs that differ from the unfavorable herringbone pattern of pentacene.
The effect of carotid artery disease on the cerebrovascular response to physiologic stimulation (1988)

Eight of 16 patients with severe carotid artery disease, but no evidence of functional or structural brain damage, had abnormal regional cerebral blood flow (rCBF) responses to physiologic stimulation of sensorimotor cortex (unilateral reduction in six, abnormal diffuse increase in two). Thus, in patients with cerebrovascular disease the rCBF responses during physiologic tasks may not be reliable indicators of local neuronal activity.
CB2receptor‐mediated migration of immune cells: it can go either way (2007)

Though many studies have examined the role of CB2 receptors in immune cell migration, it has been difficult to form definitive conclusions about the physiopathological role of these receptors in regulating immune responses and how this might be pharmacologically targeted for therapy. Do cannabinoids promote inflammation through the recruitment of immune cells, or reduce inflammation by interfering with the action of other chemoattractants? Is therapeutic intervention with an agonist or antagonist more appropriate for the reduction of inflammation? In this review, we will summarize the progress that has been made in answering these questions and outline current hypotheses.