gebhart/patrepeval huggingface dataset:
A patch‐clamp study of effects of dihydroavermectin on Ascaris muscle (1989)

1. Effects of bath-application of the anti-parasitic agent 22,23-dihydroavermectin B1a (DHAVM 20 fM to 0.2 microM) on outside-out and inside-out patches from collagenase-treated muscle membranes of Ascaris suum were examined. 2. DHAVM was tested as a gamma-aminobutyric acid (GABA) agonist by application to outside-out patches. DHAVM failed to open characteristic GABA channels even when high concentrations (20 nM to 0.2 microM) were used. These high concentrations of DHAVM also failed to potentiate opening of channels activated by application of 3-4 microM GABA; instead they acted to depress GABA-activated channel currents by reducing mean conductances and P open. 3. In outside-out patches, low concentrations of DHAVM (1 pM to 100 pM) produced opening of 0-13 channels, the number depending on the patch. The progressive opening of channels gave rise to a 'Staircase' effect. The conductance of these channels was 9-15 pS and open times were long (greater than 100 mS). Ion substitution experiments showed these channels to be permeable to Cl. The channels were not blocked by 20 microM picrotoxin. There was a long delay (greater than 15 s) between DHAVM application and channel opening; this delay and lipophilic nature of DHAVM suggested a site of action in the lipid phase of the membrane. 4. The effect of bath-application of low concentrations of DHAVM on inside-out patches was investigated under low-Ca conditions (to avoid Ca-activated Cl channels). DHAVM (20 fM to 1 nM) did not produce opening of Cl channels but produced opening after a long delay (mean 2.5 minutes) of noisy cation-selective channels which had conductances of 5-30 pS. 5. The actions of DHAVM are discussed.
Interactive three-dimensional holographic displays (1997)

Computer graphics is confined chiefly to flat images. Images may look three-dimensional (3D), and sometimes create the illusion of 3D when displayed, for example, on a stereoscopic display [16, 13, 12]. Nevertheless, when viewing an image on most display systems, the human visual system (HVS) sees a flat plane of pixels. Volumetric displays can create a 3D computer graphics image, but fail to provide many visual depth cues (e.g. shading texture gradients) and cannot provide the powerful depth cue of overlap (occlusion). Discrete parallax displays (such as lenticular displays) promise to create 3D images with all of the depth cues, but are limited by achievable resolution. Only a real-time electronic holographic ("holovideo") display [11, 6, 8, 7, 9, 21, 22, 20, 2] can create a truly 3D computer graphics image with all of the depth cues (motion parallax, ocular accommodation, occlusion, etc.) and resolution sufficient to provide extreme realism [13]. Holovideo displays promise to enhance numerous applications in the creation and manipulation of information, including telepresence, education, medical imaging, interactive design and scientific visualization.The technology of electronic interactive three-dimensional holographic displays is in its first decade. Though fancied in popular science fiction, only recently have researchers created the first real holovideo systems by confronting the two basic requirements of electronic holography: computational speed and high-bandwidth modulation of visible light. This article describes the approaches used to address these problems, as well as emerging technologies and techniques that provide firm footing for the development of practical holovideo.
Effects of Shock Vibration on Concrete (2005)

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Insulin‐Loaded Calcium Pectinate Nanoparticles: Effects of Pectin Molecular Weight and Formulation pH (2004)

Insulin-loaded calcium pectinate nanoparticles were prepared as a potential colonic delivery system by ionotropic gelation with calcium ions. The effects of pectin molecular weight (Mv) and formulation pH on the characteristics of the nanoparticles were evaluated. Commercial pectins, LM101 and LM104, with respective degrees of esterification of 36% and 28%, were depolymerized by mechanical milling to give Mv ranging from 89 to 5.6 kDa. Milled pectins did not yield nanoparticles with significantly different mean diameter and insulin association efficiency (AE) compared to nanoparticles of unmilled pectins. LM104 nanoparticles had smaller variation in mean size than the LM101 nanoparticles. Formulation pH significantly influenced the AE and stability of the nanoparticles. Increasing the pH from 2 to 3 enhanced the AE by three-fold, from 32.76% to 93.31%, at an insulin loading concentration of 80 U/mL. This increase in AE was correlated to the charge density on the pectin molecules as a function of pH. Subsequent release of associated insulin from the nanoparticles was dependent on the extent of dilution of the nanoparticle dispersion and the pH of the dissolution medium. Cross-flow filtration could be used to separate the nanoparticles from unassociated ions and molecules, without compromising the characteristics of the nanoparticles.
Induction of Vigorous Helper and Cytotoxic T Cell as well as B Cell Responses by Dendritic Cells Expressing a Modified Antigen Targeting Receptor-Mediated Internalization Pathway (2000)

Efficient Ag presentation is essential to induce effective cellular and humoral immune responses. Thus, one central goal of current immunotherapy and vaccine development is to enhance Ag presentation to induce potent and broad immune responses. Here, a novel Ag presentation strategy is developed by transducing dendritic cells (DCs) to produce an Ag for presentation as an exogenous Ag to efficiently induce both humoral and cellular immunity. The principle of this strategy is illustrated by genetically modifying DCs to secrete a model hepatitis B virus Ag fused with a cell-binding domain and to process the fusion Ag as an exogenous Ag after receptor-mediated internalization for MHC class I and II presentation. Vigorous Ag-specific CD4(+) helper and CD8(+) cytotoxic T cell, as well as B cell, responses were induced by the transduced DCs in mouse models. Thus, this novel strategy uses a receptor-mediated internalization process to efficiently induce all arms of the adaptive immunity and may provide a powerful means to develop potent vaccines and immunotherapies.
AAV2/8-mediated Correction of OTC Deficiency Is Robust in Adult but Not Neonatal Spfash Mice (2009)

Ornithine transcarbamylase (OTC) deficiency, the most common urea cycle disorder, is associated with severe hyperammonemia accompanied by a high risk of neurological damage and death in patients presenting with the neonatal-onset form. Contemporary therapies, including liver transplantation, remain inadequate with considerable morbidity, justifying vigorous investigation of alternate therapies. Clinical evidence suggests that as little as 3% normal enzyme activity is sufficient to ameliorate the severe neonatal phenotype, making OTC deficiency an ideal model for the development of liver-targeted gene therapy. In this study, we investigated metabolic correction in neonatal and adult male OTC-deficient Spf(ash) mice following adeno-associated virus (AAV)2/8-mediated delivery of the murine OTC complementary DNA under the transcriptional control of a liver-specific promoter. Substantially supraphysiological levels of OTC enzymatic activity were readily achieved in both adult and neonatal mice following a single intraperitoneal (i.p.) injection, with metabolic correction in adults being robust and life-long. In the neonates, however, full metabolic correction was transient, although modest levels of OTC expression persisted into adulthood. Although not directly testable in Spf(ash) mice, these levels were theoretically sufficient to prevent hyperammonemia in a null phenotype. This loss of expression in the neonatal liver is the consequence of hepatocellular proliferation and presents an added challenge to human therapy.
A comparison of different focus functions for use in autofocus algorithms (1985)

A number of functions for the autofocusing of microscopes and other optical instruments are to be found in the literature. In this article we compare 11 of them to determine, in an objective manner, which functions are most suitable for implementation with real-time video acquisition systems. Three different images, each representing a typical class of image, are used in the comparison. Among the best focus functions found in our study are the squared magnitude gradient, the squared Laplacian, and the normalized image standard deviation.
T Cells Expressing CD19/CD20 Bispecific Chimeric Antigen Receptors Prevent Antigen Escape by Malignant B Cells (2016)

The adoptive transfer of T cells expressing anti-CD19 chimeric antigen receptors (CARs) has shown remarkable curative potential against advanced B-cell malignancies, but multiple trials have also reported patient relapses due to the emergence of CD19-negative leukemic cells. Here, we report the design and optimization of single-chain, bispecific CARs that trigger robust cytotoxicity against target cells expressing either CD19 or CD20, two clinically validated targets for B-cell malignancies. We determined the structural parameters required for efficient dual-antigen recognition, and we demonstrate that optimized bispecific CARs can control both wild-type B-cell lymphoma and CD19(-) mutants with equal efficiency in vivo To our knowledge, this is the first bispecific CAR capable of preventing antigen escape by performing true OR-gate signal computation on a clinically relevant pair of tumor-associated antigens. The CD19-OR-CD20 CAR is fully compatible with existing T-cell manufacturing procedures and implementable by current clinical protocols. These results present an effective solution to the challenge of antigen escape in CD19 CAR T-cell therapy, and they highlight the utility of structure-based rational design in the development of receptors with higher-level complexity. Cancer Immunol Res; 4(6); 498-508. ©2016 AACR
A generic load/extract utility for data transfer between XML documents and relational databases (2002)

XML is rapidly gaining momentum in e-commerce and Internet-based information exchange, where its simplicity and custom-defined tags make it usable as a semantics-preserving data exchange format. However, to realize this potential it is necessary to be able to extract structured data from XML documents and store it in a database, as well as to generate XML documents from data extracted from a database. Although many DBMS vendors are scrambling to extend their products to handle XML, there is a need for a lightweight, DBMS- and platform-independent load/extract utility as well. In this paper, we describe such a utility that solves the following problems: (1) loading data from XML documents into relational tables with a known schema, (2) creating XML documents according to a known document type definition (DTD) from data extracted from a database, (3) generating relational schemas from XML DTDs for on-the-fly storage of XML documents, and (4) generating XML DTDs from relational schemas for on-the-fly extraction of relational data. We introduce a language to describe a mapping between an existing XML DTD and an existing relational schema and discuss some of the interesting issues arising from such a mapping.
Oncogenic Association of Specific Human Papillomavirus Types With Cervical Neoplasia<xref ref-type="fn" rid="FN1">2</xref> (1987)

Molecular hybridization analysis of human papillomavirus (HPV) DNA from 190 cervical biopsy specimens from women in the United States, Brazil, and Peru revealed viral sequences in 2 (9%) of 23 biopsy specimens of normal mature squamous epithelium, 7 (44%) of 16 biopsy specimens of metaplastic squamous epithelia, 60 (77%) of 78 cervical intraepithelial neoplasia (CIN), 57 (89%) of 64 invasive squamous carcinomas, and 8 (89%) of 9 endocervical adenocarcinomas. HPV typing by DNA hybridization revealed HPV 6 and HPV 11 sequences in metaplastic squamous epithelia, CIN I, and CIN II, but not in CIN III lesions or invasive carcinomas. HPV 16 was detected in metaplastic epithelium and in nearly half of the invasive squamous carcinomas and adenocarcinomas. It was present in 31% of CIN lesions, increasing in frequency with the severity of CIN from 20% of CIN I to 50% of CIN III. HPV 16 showed a striking difference in geographic distribution, being detected in 36% of the carcinomas from the United States compared to 64% of the carcinomas from Brazil and Peru. HPV 18 was found in metaplastic epithelia and in 17% of carcinomas but in only 1% of CIN lesions. HPV 31 was not found in metaplastic epithelium but was present in 6% of carcinomas and in 18% of CIN lesions. In addition, a group of uncharacterized HPVs, not corresponding to any of the probes used, was found in 5% of normal and metaplastic epithelia and in 18% of CIN and 19% of invasive cancers. These results suggest that individual HPV types that infect the cervix have varying degrees of oncogenic association. HPV 6 and HPV 11 appear to have very little oncogenic association, HPV 31 has low oncogenic association, and HPV 16 and HPV 18 have high oncogenic association.
An ICU protocol development and management system (2002)

Patient care is commonly managed by clinicians using general guidelines or specific protocols. Acute, severe illness may require care in an intensive care unit (ICU), with many aspects of care managed concurrently. Variation of aspects of care among patients, clinicians, ICUs and institutions is well-known, and is due to a lack of standardization in clinical decision making and the inability to implement detailed, patient-responsive protocols. Automation of protocols for specific aspects of care using computing technology and medical informatics/knowledge engineering principles offers a consistent, systematic way to implement a protocol by prompting bedside clinicians in a timely manner monitoring a patient's responses to therapeutic interventions, and monitoring the decision logic used. This paper describes a new generic system for the design and implementation of computerized protocols.
Basic Principles of MR Contrast (1995)

The use of intravenous contrast media is well established in magnetic resonance (MR) for improved diagnosis. MR differs from other imaging modalities in the complexity of signal and contrast dependence, with the method of measurement having great impact on tissue contrast. Unlike computed tomography, in which contrast depends solely on x-ray density, the signal intensity on MR is determined by differences in spin density, T1 and T2 relaxation times, diffusion, perlusion, and magnetic susceptibility. The only contrast agents in widespread clinical use at this time are paramagnetic metal ion chelates. These compounds influence tissue contrast by enhancing T1 and T2 relaxation. The effectiveness of any one agent is dependent on a number of basic parameters, including concentration, number of coordination sites, magnetic moment, distance between the ion and water protons, and correlation times. An understanding of general contrast mechanisms, principles of contrast agent design, and basic MR imaging techniques is important for proper clinical implementation and medical diagnosis.
Modulating seed β-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil (2007)

beta-Ketoacyl-acyl carrier protein (ACP) synthase II (KASII) elongates 16:0-ACP to 18:0-ACP in the plastid, where it competes with three other enzymes at the first major branch point in fatty acid biosynthesis. Despite its key metabolic location, the influence of KASII in determining seed oil composition remains unclear, in part because the biochemical consequences of the fab1-1 mutation were unresolved. Thus, fab1-1, and a newly identified knockout allele, fab1-2, were analyzed in the context of the hypothesis that modulating KASII activity is sufficient to convert the composition of a temperate seed oil into that of a palm-like tropical oil. No homozygous fab1-2 individuals were identified in progeny of self-fertilized heterozygous fab1-2 plants, approximately 1/4 of which aborted before the torpedo stage, suggesting that fab1-2 represents a complete loss of function and results in lethality when homozygous. Consistent with this hypothesis, homozygous fab1-2 plants were identified when a fab1-1 transgene was introduced, demonstrating that fab1-1 encodes an active KASII. Strong seed-specific hairpin-RNAi reductions in FAB1 expression resulted in abortion of approximately 1/4 of the embryos in an apparent phenocopy of fab1-2 homozygosity. In less severe FAB1 hairpin-RNAi individuals, embryos developed normally and exhibited a 1:2:1 segregation ratio for palmitate accumulation. Thus, early embryo development appears sensitive to elevated 16:0, whereas at later stages, up to 53% of 16:0, i.e., a 7-fold increase over wild-type levels, is tolerated. These results resolve the role of KASII in seed metabolism and demonstrate that modulation of Arabidopsis KASII levels is sufficient to convert its temperate oilseed composition to that of a palm-like tropical oil.
Classification of daily and near-daily headaches (1996)

Primary chronic daily headache can be subdivided into transformed migraine, chronic tension-type headache, hemicrania continua, and new daily persistent headache. We proposed and tested criteria in 150 consecutive outpatients with chronic daily headache. Based on preliminary analysis, we revised the criteria for transformed migraine. Using the International Headache Society criteria, 43% of the patients could not be classified; using our old criteria, 25% could not be classified; however, using our new criteria, we were able to classify 100%. Seventy-eight percent had transformed migraine, 15.3% had chronic tension-type headache, and 6.7% had other headache disorders.
Association of Lubricant Use with Women’s Sexual Pleasure, Sexual Satisfaction, and Genital Symptoms: A Prospective Daily Diary Study (2010)

The water- and silicone-based lubricants used in this study were associated with significantly higher reports of sexual pleasure and satisfaction and rarely associated with genital symptoms.
Bis(perfluoroalkyl) trioxides (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBis(perfluoroalkyl) trioxidesPhillip Gerhard. ThompsonCite this: J. Am. Chem. Soc. 1967, 89, 17, 4316–4319Publication Date (Print):August 1, 1967Publication History Published online1 May 2002Published inissue 1 August 1967https://pubs.acs.org/doi/10.1021/ja00993a012https://doi.org/10.1021/ja00993a012research-articleACS PublicationsRequest reuse permissionsArticle Views100Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Protein expression in animal cells (1994)

Part 1 The use of naturally occurring and recombinant viruses: viruses as model systems in cell biology, R.W. Compans and P.C. Roberts expression of exogenous proteins in mammalian cells with the recombinant simliki forest virus vector, V.M. Olkkonen et al recombinant sindbis virus as an expression system for cell biology, R.C. Piper et al recombinant rous sarcoma virus vectors for avian cells, G. Odorizzi and I.S. Trowbridge generation of high-titer pseudotyped retroviral vectors with very broad host range, J.-K. Lee et al SV40 virus expression vectors, H.Y. Naim and M.G. Roth use of recombinant vaccinia virus vectors for cell biology, O.A. Weisz and C.E. Machamer use of recombinant adenovirus for metabolic engineering of mammalian cells, T.C. Becker et al amplicon-based herpes simplex virus vectors, D.Y. Ho replication-defective recombinant herpes virus vectors, P.A. Johnson and T. Friedman. Part 2 The use of expression plasmids in continuous cell lines: CMV plasmid vectors for permanent lines of polarized epithelial cells, C.B. Brewer inducible protein expression using a glucocorticoid-sensitive vector, R.P. Hirt et al expression of exogenous proteins in cells with regulated secretory pathways, R.A. Chavez et al expression of foreign proteins in a human neuronal system, D.G. Cook et al homologous recombination for gene replacement in mouse cell lines, T.E. Willnow et al regulation of protein activities by fusion to steroid binding domains, T. Mattioni et al gene gun transfection of animal cells and genetic immunization, D.-C. Tang and S.A. Johnston amplification of expression plasmids in continuous CHO cell lines and analysis of transdominant mutant proteins, I. Mellman.
Microdomain Orientation of PS-b-PMMA by Controlled Interfacial Interactions (2008)

The microdomain orientation in thin films of cylinder- and lamella-forming diblock copolymers, polystyrene-block-poly(methyl methacrylate) (PS-b-PMMA), was investigated as a function of the film thickness and the composition of random copolymers composed of styrene (S) and methyl methacrylate (MMA), denoted as P(S-r-MMA), that were anchored to the substrate. Using scanning force microscopy (SFM) and grazing incidence small-angle X-ray scattering (GISAXS), the dependence of the microdomain orientation on film thickness around lattice period (or d-spacing, L0), where the microdomain orientations normal to the film surface could be achieved, showed that the optimal condition for the balanced interfacial interactions (the so-called neutrality in random copolymer) was 0.64 of PS mole fraction (XPS) for the cylindrical microdomain having narrow compositional range of P(S-r-MMA) from 0.52 to 0.72 of XPS, whereas for the lamella microdomain it was observed at XPS = 0.55 ranging extensively from 0.48 to 0.78.