gebhart/patrepeval huggingface dataset:
Plant orthologs of p300/CBP: conservation of a core domain in metazoan p300/CBP acetyltransferase-related proteins (2001)

p300 and CBP participate as transcriptional coregulators in the execution of a wide spectrum of cellular gene expression programs controlling cell differentiation, growth and homeostasis. Both proteins act together with sequence-specific transcription factors to modify chromatin structure of target genes via their intrinsic acetyltransferase activity directed towards core histones and some transcription factors. So far, p300-related proteins have been described in animals ranging from Drosophila and Caenorhabditis elegans to humans. In this report, we describe p300/CBP-like polypeptides in the plant Arabidopsis thaliana. Interestingly, homology between animal and plant p300/CBP is largely restricted to a C-terminal segment, about 600 amino acids in length, which encompasses acetyltransferase and E1A-binding domains. We have examined whether this conservation in sequence is paralleled by a conservation in function. The same amino acid residues critical for acetyltransferase activity in human p300 are also critical for the function of one of the plant orthologs. Remarkably, plant proteins bind to the adenovirus E1A protein in a manner recapitulating the binding specificity of mammalian p300/CBP. The striking conservation of an extended segment of p300/CBP suggests that it may constitute a functional entity fulfilling functions that may be essential for all metazoan organisms.
Phase I trial of a novel human monoclonal antibody mAb216 in patients with relapsed or refractory B-cell acute lymphoblastic leukemia (2011)

Treatment with monoclonal antibody 216 in combination with vincristine is feasible and well tolerated in patients with relapsed or refractory B-cell acute lymphoblastic leukemia. Binding of monoclonal antibody 216 to leukemic blasts was efficient, and favorable early responses were observed.
Antibacterial Activity of and Resistance to Small Molecule Inhibitors of the ClpP Peptidase (2013)

There is rapidly mounting evidence that intracellular proteases in bacteria are compelling targets for antibacterial drugs. Multiple reports suggest that the human pathogen Mycobacterium tuberculosis and other actinobacteria may be particularly sensitive to small molecules that perturb the activities of self-compartmentalized peptidases, which catalyze intracellular protein turnover as components of ATP-dependent proteolytic machines. Here, we report chemical syntheses and evaluations of structurally diverse β-lactones, which have a privileged structure for selective, suicide inhibition of the self-compartmentalized ClpP peptidase. β-Lactones with certain substituents on the α- and β-carbons were found to be toxic to M. tuberculosis. Using an affinity-labeled analogue of a bioactive β-lactone in a series of chemical proteomic experiments, we selectively captured the ClpP1P2 peptidase from live cultures of two different actinobacteria that are related to M. tuberculosis. Importantly, we found that the growth inhibitory β-lactones also inactivate the M. tuberculosis ClpP1P2 peptidase in vitro via formation of a covalent adduct at the ClpP2 catalytic serine. Given the potent antibacterial activity of these compounds and their medicinal potential, we sought to identify innate mechanisms of resistance. Using a genome mining strategy, we identified a genetic determinant of β-lactone resistance in Streptomyces coelicolor, a non-pathogenic relative of M. tuberculosis. Collectively, these findings validate the potential of ClpP inhibition as a strategy in antibacterial drug development and define a mechanism by which bacteria could resist the toxic effects of ClpP inhibitors.
A Turn‐On Fluorescent Probe for Highly Selective and Sensitive Detection of Palladium (2016)

Abstract A novel turn‐on fluorescent probe for the detection of palladium has been designed. The probe can selectively and sensitively detect palladium in solution, and the limit of detection was calculated to be 11.4 nmol·L −1 . Furthermore, the probe was successfully used for fluorescence imaging of palladium in living cells.
Topical insulin in wound healing: a randomised, double-blind, placebo-controlled trial (1999)

Two studies were carried out to assess the relative roles of insulin and zinc in the acceleration of wound healing. In the first study, six diabetic and five non-diabetic human volunteers had two uniform cuts created, one on each forearm. One forearm wound was treated with topical regular insulin (Iletin-II) and the other with normal saline four times a day until healed. Treatment was double-blind and forearms were assigned randomly. The wounds treated with insulin healed 2.4 +/- 0.8 days faster than the wounds treated with saline (P < 0.001 by paired t-test). Zinc is used to crystallise insulin. When wounds are treated with insulin, they are therefore also being treated with zinc. If insulin accelerates wound healing, it is not clear if the increase in the rate of healing would be due to insulin (a known growth factor), the zinc it contains, or a combination of the two. The second study used a randomised, double-blind, placebo-controlled design to compare the efficacy of insulin with that of a solution containing the same amount of zinc in accelerating the healing of standardised wounds in rats and humans. Although these pilot investigations did not have the power to define the relative roles of insulin and zinc with accuracy, the results suggest that zinc does play a role in the wound healing process. It is concluded that topical insulin accelerates wound healing in humans. More importantly, however, this study describes a method of creating uniform wounds in humans acceptable to an institutional review board, thus solving one of the major impediments to the scientific evaluation of human wound healing.
Permanencia y abandono terapéutico en un centro de servicios psicológicos (2012)

El presente estudio tuvo como propósito describir las características de los motivos de permanencia y abandono terapéutico de los consultantes de la Unidad de Servicios Psicológicos de la Universidad Católica de Colombia entre los años 2007 y 2008. El tipo de investigación fue de tipo descriptivo. Los participantes fueron los consultantes que asistieron a la Unidad de Servicios Psicológicos de la Universidad Católica de Colombia entre los años 2007 y 2008. El muestreo fue de tipo censal puesto que se tomaron todos los usuarios y se analizó la información de los que finalmente respondieron los instrumentos así como las historias clínicas de los usuarios. Los resultados permitieron identificar que el principal motivo por el cual los usuarios abandonan el servicio está relacionado con dificultades económicas para su desplazamiento. Las variables analizadas para permanecer o abandonar el servicio con respecto a la institución, al proceso terapéutico, al consultante, al terapeuta y a la relación consultante - terapeuta, presentan un comportamiento similar.
Intra-Familial Tests of Association between Familial Idiopathic Scoliosis and Linked Regions on 9q31.3–q34.3 and 16p12.3–q22.2 (2012)

Nominally significant linkage results were found for markers in both candidate regions. Results from intra-familial tests of association and tiled regression corroborated the linkage findings and identified possible candidate genes suitable for follow-up with next-generation sequencing in these same families. Candidate genes that met our prioritization criteria included FAM129B and CERCAM on chromosome 9 and SYT1, GNAO1, and CDH3 on chromosome 16.
Nomenclature for factors of the HLA system, 1994 (1994)

The WHO Nomenclature Committee for factors of the HLA system met in Strasbourg in March 1994 at the European Foundation for Immunogenetics Conference to consider additions and revisions to the nomenclature of specificities defined by both molecular and serological techniques following the principles established in previous reports (1, 3, 3, 4, 5 , 6, 7, 8, 9, 10, 11).
Human MxB Protein, an Interferon-α-inducible GTPase, Contains a Nuclear Targeting Signal and Is Localized in the Heterochromatin Region beneath the Nuclear Envelope (1996)

Interferon-inducible Mx proteins belong to the family of large GTPases and are highly homologous with dynamins within their GTP-binding domain. Cytoplasmically localized human MxA protein mediates resistance to influenza and several other viruses, whereas human MxB protein has not been found to have any antiviral activity. Here we show that MxB protein is found both in the cytoplasm and in the nucleus, where it is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. Transfection experiments in COS cells of N-terminally deleted MxB constructs revealed a functional nuclear localization signal within the first 24 N-terminal amino acids. Nuclear 78-kDa and cytoplasmic 76-kDa forms of MxB protein were found in all of the cell lines studied and in human peripheral blood mononuclear cells. MxB protein proved to be a functional GTPase with activity comparable to that of MxA protein. N-terminally truncated (delta1-82) MxB protein lacking both the nuclear localization signal and a proline-rich domain had almost completely lost its GTPase activity. Analysis of peripheral blood mononuclear cells suggested that MxB protein expression is strictly regulated by interferon-alpha. This is the first documentation that human Mx protein resides in the nucleus. It also emphasizes that there are considerable differences in the localization and structure of functional domains within Mx proteins.
Plasma lipids and lipoproteins in subtypes of stroke (1992)

We determined plasma lipid and lipoprotein concentrations in 131 patients (95 males, 36 females, mean age 71 years) 6 months after acute stroke onset. Compared to a reference population, the alterations of plasma lipid concentrations in stroke patients were moderate. However, the stroke patients had higher levels of triglyceride and lipoprotein (a) and lower concentrations of cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol. Patients with different subtypes of stroke showed significant differences when compared with each other by analysis of covariance (with adjustment for age, sex, smoking, hypertension and diabetes). Patients with carotid or vertebral artery disease had the higher levels of cholesterol, triglyceride and apolipoprotein B, whereas high density lipoprotein triglyceride concentrations were higher in patients with cardioembolic infarcts.
The Future of Seawater Desalination: Energy, Technology, and the Environment (2011)

In recent years, numerous large-scale seawater desalination plants have been built in water-stressed countries to augment available water resources, and construction of new desalination plants is expected to increase in the near future. Despite major advancements in desalination technologies, seawater desalination is still more energy intensive compared to conventional technologies for the treatment of fresh water. There are also concerns about the potential environmental impacts of large-scale seawater desalination plants. Here, we review the possible reductions in energy demand by state-of-the-art seawater desalination technologies, the potential role of advanced materials and innovative technologies in improving performance, and the sustainability of desalination as a technological solution to global water shortages.
4.5 A 16nm FinFET heterogeneous nona-core SoC complying with ISO26262 ASIL-B: Achieving 10−7 random hardware failures per hour reliability (2016)

The role of car information systems (commonly referred to as car infotainment) is expanding from dedicated navigation systems to joint car-cockpit systems, including the dashboard meter, telematics for the internet/cloud, and advanced driver-assistance systems (ADASs), such as adaptive cruise control and a pre-crash safety system. The expanding role for car information systems requires higher computational performance, but also safety mechanisms which prevent serious accidents. This paper presents an SoC for the next generation of car infotainment, achieving high performance powered by nine heterogeneous CPUs and a high level of safety, complying with ISO26262 ASIL-B. It has two key features: 1) Run-time test for functional safety, which can detect wear-out faults, such as random fault, time-dependent dielectric breakdown, and electromigration; 2) A killer-droop (critical voltage droop) monitor with droop prediction, which can avoid a delay fault caused by voltage droop.
In Vitro HIV-Specific CTL Activity from HIV-Seropositive Individuals Is Augmented by Interleukin-12 (IL-12) (2001)

IL-12 production is reduced in HIV infection, and recombinant human IL-12 (rhIL-12) augments in vitro HIV-specific proliferative responses in PBMC from HIV-seropositive individuals. To determine whether rhIL12 could also augment HIV-specific CTL responses we studied 41 HIV-seropositive individuals. Recombinant hIL-12 increased the detectable in vitro HIV-specific CD8 CTL activity of PBMC taken from HIV-seropositive individuals with CD4 counts >500 cells/microl and from some individuals with lower CD4 counts. IL-12 increased cell recovery in cultures of PBMC from HIV-seropositive individuals with CD4 counts >500 cells/microl and also increased the precursor CTL frequency. However, the increase in HIV-specific CTL activity was not due to IL-2 or IFN-gamma production or an increase in the number of cells with surface markers characteristic of CTL effector cells. This study demonstrates that rhIL-12 augments in vitro HIV-specific CTL activity and provides evidence to justify further investigation within clinical trials of this cytokine in HIV infection.
Inappropriate Recruitment and Activity by the Src Homology Region 2 Domain-Containing Phosphatase 1 (SHP1) Is Responsible for Receptor Dominance in the SHIP-Deficient NK Cell (2007)

We have previously demonstrated that the NKR repertoire is profoundly disrupted by SHIP deficiency. This repertoire disruption is characterized by receptor dominance where inhibitory signals from 2B4 repress killing of complex targets expressing MHC class I and activating ligands. In this study, we examine the molecular basis of receptor dominance in SHIP-/- NK cells. In this study, we show that in SHIP-/- NK cells there is a pronounced bias toward the 2B4 long isoform. We have also characterized signaling molecules recruited to 2B4 in SHIP-/- NK cells. Interestingly, we find that approximately 10- to 16-fold more Src homology region 2 domain-containing phosphatase 1 (SHP1) is recruited to 2B4 in SHIP-/- NK cells when compared with wild type. Consistent with SHP1 overrecruitment, treatment with sodium orthovanadate or a novel inhibitor with micromolar activity against SHP1 restores the ability of SHIP-/- NK cells to kill Rae1+ RMA and M157+ targets. These findings define the molecular basis for hyporesponsiveness by SHIP-deficient NK cells.
Implantation Success and Infection in Cardiovascular Implantable Electronic Device Procedures Utilizing an Antibacterial Envelope (2010)

CIED procedures that utilized an antibacterial envelope had a high rate of CIED implantation success (>99%). Although the follow-up to date is short, there was also a low rate of infection (<0.50%) in this population at high risk for CIED infection.
Properties and Characterization of Al2O3 and SiO2-TiO2 Pillared Saponite (1993)

Abstract A saponite pillared with a single (Al 2 O 3 ) or a mixed (SiO 2 -TiO 2 ) oxide exhibited basal spacings of 16–19 and 30–40Å, respectively. The pillared structures were found to be stable up to 700°C. Water, nitrogen, and high resolution argon adsorption were used to study the effect of thermal treatments on surface chemistry, pore structure, and surface area of these pillared clays. The pillared saponites exhibited a hydrophobic behavior at temperatures &gt; 500°C, whereas such behavior was observed at ≥300°C for montmorillonite. Most of the micropores in the Al 2 O 3 pillared clays were &lt; 10 Å, whereas the SiO 2 -TiO 2 pillared clays showed a broad distribution of pores in both micropore and mesopore regions. The SiO 2 -TiO 2 pillared samples possessed higher surface area compared with Al 2 O 3 pillared clays. The percent decrease in surface area was smaller for pillared saponites compared with pillared montmorillonites when calcined from 300° to 700°C, indicating a higher thermal stability of the former. The pillared clays were also characterized by solid state 27 Al and 29 Si magic-angle spinning nuclear magnetic resonance (MAS/NMR) spectroscopy. There was no direct evidence of cross-linking (covalent bonding between the clay layer and pillar) in montmorillonite irrespective of the types of pillars. In saponite, however, a significant structural modification took place. 27 Al spectra of Al 2 O 3 pillared saponite heated at ≥300°C appear to indicate an increase in Al VI as a result, at least in part, of initiation of hydrolytic splitting of Si-O-Al bonds. The actual release of Al from the tetrahedral sheet probably occurred at a temperature &gt; 500°C and completed around 700°C with the formation of Si-O-Si linkages. The decreased intensity of peak due to Si(1Al) in 29 Si spectra of the sample heated at 700°C corroborates the 27 Al MAS/NMR results. Additionally, the 29 Si spectra indicated a cross-linking between SiO 4 (clay sheet) with Al 2 O 3 pillars, which could be achieved by inverting some silica tetrahedra into the interlayer. 27 Al and 29 Si spectra of SiO 2 TiO 2 pillared saponite also showed the trend similar to that exhibited by Al 2 O 3 pillared saponite, indicating that the crystal chemistry of the host may be more important than the nature of pillars in the structural modification and cross-linking behavior of thermally treated pillared clays.
<title>Effects of nonlinear parameters in feedback joint transform correlator (I)</title> (1993)

We report that nonlinear parameters effect the characteristics of the optical pattern recognition of a feedback joint transform correlator (FJTC) which is based on a joint transform correlator (JTC). This system incorporates the optically addressed ferroelectric liquid crystal spatial light modulator named LAPS-SLM. Nonlinear parameters in the FJTC are a threshold level of the LAPS-SLM to record the binary joint power spectrum (JPS), and a feedback transfer function from the correlation plane to the input plane. It is important to control the threshold level of the binary JPS. But the FJTC does not always need the optimum condition where the FJTC recognizes the signal image correctly at the initial recognition.
Identification of human myometrial target genes of the c-Jun NH2-terminal kinase (JNK) pathway: the role of activating transcription factor 2 (ATF2) and a novel spliced isoform ATF2-small (2005)

Activating transcription factor 2 (ATF2), a ubiquitously expressed member of the basic region leucine zipper (bZIP) family of transcription factors activated by mitogen activated protein kinase (MAPK) pathways, is important in the mediation of cellular stress responses, development and transformation. We have previously reported the differential expression of active ATF2 in the human myometrium throughout pregnancy and labour, and identified and partially characterized a novel splice variant ATF2-small (ATF2-sm). To further understand the role of these factors in the myometrium, we have used gene microarrays to define the target genes in cultured myometrial cells stably-transfected with ATF2 and ATF2-sm cDNAs. Many of the genes identified appear to have potential roles in regulating myometrial function and include proteins involved in G-protein receptor signalling, cytokine signalling, transcriptional regulation, cell-cycle control, formation of the extracellular matrix and cytoskeletal architecture. ATF2 was found to affect the expression of 204 genes; 113 being up-regulated and 91 down-regulated whereas the novel ATF2-sm factor altered the expression of 55 genes; expression was increased in 29 cases and decreased in 26. A further 25 genes affected by ATF2-sm were identified by suppression subtractive hybridisation (SSH). Notably, the genes affected by ATF2 and ATF2-sm appear to belong to discrete groups: only two genes were affected by both factors.
A New Mutation (Arg251 Trp) in the Ca2+ Binding Site of Factor X Protease Domain Appears to be Responsible for the Defect in the Extrinsic Pathway Activation of Factor X Padua (2004)

Factor X Padua, first described a few years ago, is characterized by a defect only in the extrinsic system. In this present paper, the molecular basis for this peculiar defect is investigated. Polymerase chain reaction amplification and direct sequencing of the entire FX coding sequence and of exon-intron junctions detected in the proposita a C-to-T translocation in exon 8 of nucleotide 875 at the homozygous level. This resulted in the substitution of tryptophan for arginine 251. A niece of the proposita was shown to be heterozygous for the abnormality. Molecular modeling suggested that the mutation does not alter significantly folding and stability of the protein but may be involved in the Ca2+ binding site.
UV-B induced changes of phenol composition and antioxidant activity in black currant fruit (Ribes nigrum L.) (2012)

Information on UV-B elicitor mediated changes on phenolic composition and antioxidant activity of black currant (Ribes nigrum L.) are scanty. In the present study physiological ripe black currant fruits were harvested and exposed to UV-B radiation with different exposure and adaptation times. The influence of UV-B on phenolic profile and quantitative composition as well as on the corresponding antioxidant activity was investigated. Antioxidant activity was screened with electron spin resonance spectrometry (ESR), while phenolic compound composition was conducted by HPLC analysis. Total phenol content and phenolic composition (flavonols, anthocyanins, hydroxycinnamic and hydroxybenzoic acids) increased to a large extent during UV-B treatment, irrespective of the adaptation time. Anthocyanins are concluded to absorb UV radiation within a short time, meanwhile flavonols and phenolic acids are assumed to have an impact on antioxidant protection of UV-B mediated tissue damage. Moreover, antioxidant activity significantly correlated with different phenolic compounds and increased to a similar extent by UV-B exposure.
On the Effect of Surface Roughness on the Vapor Flow Under Leidenfrost-Levitated Droplets (1995)

In this paper a theoretical investigation is reported on the effect of surface roughness on the phenomenon of Leidenfrost-levitation of droplets above a hot surface. The problem is solved first approximately using a macroscopic approach in which the roughness is replaced by a semi-empirical slip condition of the Beavers-Joseph type. Next, a microscopic model which determines the vapor flow in the close vicinity of the rough surface is solved numerically. Three basic periodic roughnesses are examined: triangular, rectangular and semi-cylindrical. The effect of the relative size of the droplet and the roughness elements on the vapor flow is investigated in the course of the study.
Real-time monitoring of the TVA power system (1994)

The Tennessee Valley Authority (TVA) is experimenting with one of the many concepts for the uses of phasor measurements, that of utilizing phasor measurement units (PMUs) as a state estimation system. However, the term state estimation no longer applies; what is being performed is a state determination in near real-time.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>