gebhart/patrepeval huggingface dataset:
Can the biology of VEGF and haem oxygenases help solve pre-eclampsia? (2009)

Pre-eclampsia, a pregnancy-specific multi-organ syndrome characterized by widespread endothelial damage, is a new risk factor for cardiovascular disease. No therapies exist to prevent or treat this condition, even to achieve a modest improvement in pregnancy length or birth weight. Co-administration of soluble VEGFR-1 [VEGF (vascular endothelial growth factor) receptor-1; more commonly known as sFlt-1 (soluble Fms-like tyrosine kinase-1)] and sEng (soluble endoglin) to pregnant rats elicits severe pre-eclampsia-like symptoms. These two anti-angiogenic factors are increased dramatically prior to the clinical onset of pre-eclampsia and are quite possibly the 'final common pathway' responsible for the accompanying signs of hypertension and proteinuria as they can be reversed by VEGF administration in animal models. HO-1 (haem oxygenase-1), an anti-inflammatory enzyme, and its metabolite, CO (carbon monoxide), exert protective effects in several organs against oxidative stimuli. In a landmark publication, we showed that the HO-1 pathway inhibits sFlt-1 and sEng in cultured cells and human placental tissue explants. Both CO and NO (nitric oxide) promote vascular homoeostasis and vasodilatation, and activation of VEGFR-1 or VEGFR-2 induced eNOS (endothelial nitric oxide synthase) phosphorylation, NO release and HO-1 expression. Our studies established the HO-1/CO pathway as a negative regulator of cytokine-induced sFlt-1 and sEng release and eNOS as a positive regulator of VEGF-mediated vascular morphogenesis. These findings provide compelling evidence for a protective role of HO-1 in pregnancy and identify it as a target for the treatment of pre-eclampsia. Any agent that is known to up-regulate HO-1, such as statins, may have potential as a therapy. Any intervention achieving even a modest prolongation of pregnancy or amelioration of the condition could have a significant beneficial health impact worldwide.
Colonic transit scintigraphy labeled activated charcoal compared with ion exchange pellets. (1997)

Activated charcoal is a suitable alternative to resin pellets when delivered in a methacrylate-coated, delayed-release capsule to the colon for measurement of transit by scintigraphy.
Engineering human prolactin to bind to the human growth hormone receptor (1990)

A strategy of iterative site-directed mutagenesis and binding analysis was used to incorporate the receptor-binding determinants from human growth hormone (hGH) into the nonbinding homolog, human prolactin (hPRL). The complementary DNA for hPRL was cloned, expressed in Escherichia coli, and mutated to introduce sequentially those substitutions from hGH that were predicted by alanine-scanning mutagenesis and other studies to be most critical for binding to the hGH receptor from human liver. After seven rounds of site-specific mutagenesis, a variant of hPRL was obtained containing eight mutations with an association constant for the hGH receptor that was increased more than 10,000-fold. This hPRL variant binds one-sixth as strongly as wild-type hGH, but shares only 26 percent overall sequence identity with hGH. These studies show the feasibility of recruiting receptor-binding properties from distantly related and functionally divergent hormones and show that a detailed functional database can be used to guide the design of a protein-protein interface in the absence of direct structural information.
Basic & Clinical Pharmacology (2018)

Looks at the essential conepts in the science of pharmacology and its application to clinical practice.
Imaging of HSV-tk Reporter Gene Expression: Comparison Between [18F]FEAU, [18F]FFEAU, and Other Imaging Probes (2008)

For HSV1-tk reporter systems that require a 1- to 4-h PET paradigm, HSV1-tk-[18F]FEAU is the current top contender.
Some Patterns for Parametric Modeling (2007)

Patterns express generic solutions to a well-described problem. In parametric modeling patterns can be used to describe a ÂtacticalÂ level of work, above mechanics and below design. We describe three patterns and show how they can be used through an example model.
Distributed array data management on NUMA multiprocessors (2002)

Management of program data to reduce false sharing and improve locality is critical for scaling performance on NUMA multiprocessors. We use HPF-like directives to partition and place arrays in data-parallel applications on Hector, a shared-memory NUMA multiprocessor. We present experimental results that demonstrate the magnitude of the performance improvement attainable when our proposed array management schemes are used instead of the operating system management policies. We then describe a compiler system for automating the partitioning and placement of arrays. The compiler uses a number of techniques that exploit Hector's shared memory architecture to efficiently implement distributed arrays. Experimental results from a prototype implementation demonstrate the effectiveness of these techniques.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Beyond HPV: Oncomirs as new players in cervical cancer (2008)

MicroRNAs (miRNAs) are a recently discovered family of 18-24 nucleotide non-coding RNAs that can negatively regulate target mRNAs. All studied multicellular eukaryotes utilize miRNAs to regulate basic cellular functions including proliferation, differentiation, and death. It is now apparent that abnormal miRNA expression is a common feature of human malignancies. This review discusses the various cancer-relevant miRNAs (oncomirs) especially in cervical tumorigenesis and the potential role of oncomirs as therapeutic agents and targets for the treatment of cervical cancer.
Transforming growth factor-beta protein and mRNA in glomeruli in normal and diseased human kidneys. (1993)

These results indicate that mature TGF-beta and TGF-beta-LAP are localized in association with the matrix components of GBM or mesangium, and with immune deposits in human glomeruli. Glomerular expression of TGF-beta is enhanced in human glomerular diseases, and may contribute to the mesangial matrix increase.
Reaction of diisobutylaluminum hydride with selected organic compounds containing representative functional groups (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReaction of diisobutylaluminum hydride with selected organic compounds containing representative functional groupsNung Min Yoon and Young Soo GyoungCite this: J. Org. Chem. 1985, 50, 14, 2443–2450Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/jo00214a009https://doi.org/10.1021/jo00214a009research-articleACS PublicationsRequest reuse permissionsArticle Views5580Altmetric-Citations121LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
TWINKLE: a low earth orbit visible and infrared exoplanet spectroscopy observatory (2018)

Twinkle is a space mission designed for visible and near-IR spectroscopic observations of extrasolar planets. Twinkle’s highly stable instrument will allow the photometric and spectroscopic observation of a wide range of planetary classes around different types of stars, with a focus on bright sources close to the ecliptic. The planets will be observed through transit and eclipse photometry and spectroscopy, as well as phase curves, eclipse mapping and multiple narrow-band time-series. The targets observed by Twinkle will be composed of known exoplanets mainly discovered by existing and upcoming ground surveys in our galaxy (e.g. WASP, HATNet, NGTS and radial velocity surveys) and will also feature new discoveries by space observatories (K2, GAIA, Cheops, TESS). Twinkle is a small satellite with a payload designed to perform high-quality astrophysical observations while adapting to the design of an existing Low Earth Orbit commercial satellite platform. The SSTL-300 bus, to be launched into a low- Earth sun-synchronous polar orbit by 2019, will carry a half-meter class telescope with two instruments (visible and near-IR spectrographs - between 0.4 and 4.5&mu;m - with resolving power R~300 at the lower end of the wavelength scale) using mostly flight proven spacecraft systems designed by Surrey Satellite Technology Ltd and a combination of high TRL instrumentation and a few lower TRL elements built by a consortium of UK institutes. The Twinkle design will enable the observation of the chemical composition and weather of at least 100 exoplanets in the Milky Way, including super-Earths (rocky planets 1-10 times the mass of Earth), Neptunes, sub-Neptunes and gas giants like Jupiter. It will also allow the follow-up photometric observations of 1000+ exoplanets in the visible and infrared, as well as observations of Solar system objects, bright stars and disks.
Experimental studies and modeling on concentration polarization in forward osmosis (2010)

Concentration polarization (CP) is an important issue in forward osmosis (FO) processes and it is believed that the coupled effect of dilutive internal CP (DICP) and concentrative external CP (CECP) limits FO flux. The objective of this study was to distinguish individual contribution of different types of DICP and CECP via modeling and to validate it by pilot studies. The influence of DICP/CECP on FO flux has been investigated in this study. The CP model presented in this work was derived from a previous study and evaluated by bench-scale FO experiments. Experiments were conducted with drinking water as the feed and NaCl/MgSO(4) as draw solutions at different concentrations and velocities. Modeling results indicated that DICP contributed to a flux reduction by 99.9% for 0.5 M NaCl as a draw solution although the flow pattern of both feed and draw solutions was turbulent. DICP could be improved via selection of the draw solution. The modeling results were well fit with the experimental data. It was concluded that the model could be used for selection of the draw solution and prediction of water flux under similar situation. A draw solution with greater diffusion coefficient or a thinner substrate of an asymmetric FO membrane resulted in a higher flux.
Recent Developments in Linker Design and Application (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
STRUCTURE AND FUNCTION OF THE β3-ADRENERGIC RECEPTOR (1997)

The beta 3 subtype of adrenaline and noradrenaline receptors has now been extensively characterized at the structural and functional levels. Ligand binding and adenylyl cyclase activation studies helped define a beta-adrenergic profile that is quite distinct from that of the beta 1- and beta 2-adrenergic receptors, but strongly reminiscent of most of the "atypical" responses reported in earlier pharmacologic studies. Human, other large mammal, and rodent receptors share most of the characteristic beta 3 properties, although obvious species-specific differences have been identified. Recently, the incidence of a naturally occurring variant of the human beta 3-adrenergic receptor was shown to be correlated with hereditary obesity in Pima Indians and in Japanese individuals, and in Western obese patients with increased dynamic capacity to add on weight and develop non-insulin-dependent diabetes mellitus (NIDDM). A mild weight increase was also shown to develop in female, but not male, mice in which the beta 3 receptor gene was disrupted. Taken together, these results now provide a consistent picture of an important role of the beta 3-adrenoceptor in the regulation of lipid metabolism and as an obvious target for drugs to treat some forms of obesity.
Potassium Channel KIR4.1 as an Immune Target in Multiple Sclerosis (2012)

KIR4.1 is a target of the autoantibody response in a subgroup of persons with multiple sclerosis. (Funded by the German Ministry for Education and Research and Deutsche Forschungsgemeinschaft.).
Biodistribution and toxicity of engineered gold nanoparticles: a review of in vitro and in vivo studies (2010)

Recent advances in wet chemical synthesis and biomolecular functionalization of gold nanoparticles have led to a dramatic expansion of their potential biomedical applications, including biosensorics, bioimaging, photothermal therapy, and targeted drug delivery. As the range of gold nanoparticle types and their applications continues to increase, human safety concerns are gaining attention, which makes it necessary to better understand the potential toxicity hazards of these novel materials. Whereas about 80 reports on the in vivo biodistribution and in vitro cell toxicity of gold nanoparticles are available in the literature, there is lack of correlation between both fields and there is no clear understanding of intrinsic nanoparticle effects. At present, the major obstacle is the significant discrepancy in experimental conditions under which biodistribution and toxicity effects have been evaluated. This critical review presents a detailed analysis of data on the in vitro and in vivo biodistribution and toxicity of most popular gold nanoparticles, including atomic clusters and colloidal particles of diameters from 1 to 200 nm, gold nanoshells, nanorods, and nanowires. Emphasis is placed on the systematization of data over particle types and parameters, particle surface functionalization, animal and cell models, organs examined, doses applied, the type of particle administration and the time of examination, assays for evaluating gold particle toxicity, and methods for determining the gold concentration in organs and distribution of particles over cells. On the basis of a critical analysis of data, we arrive at some general conclusions on key nanoparticle parameters, methods of particle surface modification, and doses administered that determine the type and kinetics of biodistribution and toxicity at cellular and organismal levels (197 references).
Effect of Theophylline on Ischemically Induced Hippocampal Damage in Mongolian Gerbils: A Behavioral and Histopathological Study (1987)

We investigated the influence of the adenosine antagonist theophylline on the degree of hippocampal cell damage in the Mongolian gerbil following brief periods of forebrain ischemia. Male gerbils were randomly divided into nine groups. Ten minutes before surgery, four groups, which were later subjected to 1, 2, 3, or 5 min of bilateral carotid occlusion under halothane anesthesia, received theophylline (30 mg/kg, p.o.). Four groups served as nontreated ischemic controls; the ninth group was used to measure theophylline serum concentration. Neurological symptoms were classified by using a behavioral score. Fourteen days after ischemia, the brains were removed, and the hippocampus was histologically examined "blind" for the degree of cell damage in the CA1 sector, which was expressed as a semiquantitative histopathological score. There were no behavioral or histological abnormalities in either the control or theophylline group with 1 min of ischemia. With increasing duration of ischemia, the neurological symptoms worsened and the number of necrotic pyramidal cells increased significantly. The pretreatment with theophylline only moderately aggravated the neurological symptoms, whereas it enhanced the ischemic cell damage significantly. The results are discussed with respect to recent findings that theophylline may block putatively protective effects of endogenous adenosine, whose concentration in the brain is known to rise significantly during ischemia.
Modeling for text compression (1989)

The best schemes for text compression use large models to help them predict which characters will come next. The actual next characters are coded with respect to the prediction, resulting in compression of information. Models are best formed adaptively, based on the text seen so far. This paper surveys successful strategies for adaptive modeling that are suitable for use in practical text compression systems. The strategies fall into three main classes: finite-context modeling, in which the last few characters are used to condition the probability distribution for the next one; finite-state modeling, in which the distribution is conditioned by the current state (and which subsumes finite-context modeling as an important special case); and dictionary modeling, in which strings of characters are replaced by pointers into an evolving dictionary. A comparison of different methods on the same sample texts is included, along with an analysis of future research directions.
Disulfide bond structures of IgG molecules (2012)

The disulfide bond structures established decades ago for immunoglobulins have been challenged by findings from extensive characterization of recombinant and human monoclonal IgG antibodies. Non-classical disulfide bond structure was first identified in IgG4 and later in IgG2 antibodies. Although, cysteine residues should be in the disulfide bonded states, free sulfhydryls have been detected in all subclasses of IgG antibodies. In addition, disulfide bonds are susceptible to chemical modifications, which can further generate structural variants such as IgG antibodies with trisulfide bond or thioether linkages. Trisulfide bond formation has also been observed for IgG of all subclasses. Degradation of disulfide bond through β-elimination generates free sulfhydryls disulfide and dehydroalanine. Further reaction between free sulfhydryl and dehydroalanine leads to the formation of a non-reducible cross-linked species. Hydrolysis of the dehydroalanine residue contributes substantially to antibody hinge region fragmentation. The effect of these disulfide bond variations on antibody structure, stability and biological function are discussed in this review.
Glucocorticoid receptor inhibits transforming growth factor-β signaling by directly targeting the transcriptional activation function of Smad3 (1999)

The transforming growth factor-beta (TGF-beta) family of cytokines and glucocorticoids regulate diverse biological processes through modulating the expression of target genes. Here we report that glucocorticoid receptor (GR) represses TGF-beta transcriptional activation of the type-1 plasminogen activator inhibitor (PAI-1) gene in a ligand-dependent manner. Similarly, GR represses TGF-beta activation of the TGF-beta responsive sequence containing Smad3/4-binding sites. Using mammalian two-hybrid assays, we demonstrate that GR inhibits transcriptional activation by both Smad3 and Smad4 C-terminal activation domains. Finally, we show that GR interacts with Smad3 both in vitro and in vivo. These results suggest a molecular basis for the cross-regulation between glucocorticoid and TGF-beta signaling pathways.
Online CD Performance Monitoring and Automatic Alignment Correction (2011)

Cross Directional (CD) alignment is a key component used in modeling and control of papermaking processes. It specifies the spatial relationship between the center of individual CD actuators and the center of the corresponding measurement responses. A poor CD alignment may cause the quality degradation of the finished product and unnecessary CD actuation at higher spatial frequencies which in turn can lead to higher production cost. This paper presents techniques for monitoring CD performance and correcting poor CD alignment in closed-loop. A revised accumulated sum algorithm is used to detect the onset of CD performance degradation and the pseudo random binary sequence (PRBS) is used in the identification of new CD alignment. The techniques presented in this paper have been implemented in industrial CD control systems and validated in multiple paper mills.
Production, characterization and application of activated carbon from brewer’s spent grain lignin (2009)

Different types of activated carbon were prepared by chemical activation of brewer's spent grain (BSG) lignin using H(3)PO(4) at various acid/lignin ratios (1, 2, or 3g/g) and carbonization temperatures (300, 450, or 600 degrees C), according to a 2(2) full-factorial design. The resulting materials were characterized with regard to their surface area, pore volume, and pore size distribution, and used for detoxification of BSG hemicellulosic hydrolysate (a mixture of sugars, phenolic compounds, metallic ions, among other compounds). BSG carbons presented BET surface areas between 33 and 692 m(2)/g, and micro- and mesopores with volumes between 0.058 and 0.453 cm(3)/g. The carbons showed high capacity for adsorption of metallic ions, mainly nickel, iron, chromium, and silicon. The concentration of phenolic compounds and color were also reduced by these sorbents. These results suggest that activated carbons with characteristics similar to those commercially found and high adsorption capacity can be produced from BSG lignin.
Cloning and structure of the Bacillus subtilis aspartate transcarbamylase gene (pyrB). (1986)

The Bacillus subtilis gene (pyrB), which encodes aspartate transcarbamylase (ATCase), was cloned on a HindIII restriction endonuclease fragment inserted into the pUC13 plasmid vector. B. subtilis pyrB was expressed in Escherichia coli, as judged by complementation of E. coli pyrB mutants and production of enzyme that was specifically inhibited by antibody directed against B. subtilis ATCase. The extent of expression was strongly dependent on the orientation of the inserted DNA in the vector, which suggested that transcription was initiated from vector-borne (rather than B. subtilis) promoters. The entire 1098-base pair HindIII fragment of B. subtilis DNA was sequenced by the Maxam-Gilbert method. The amino acid sequence of B. subtilis ATCase was deduced from a 305-codon open reading frame and agreed very well with analyses of the purified enzyme. Comparison of the sequence of B. subtilis ATCase with that of E. coli ATCase catalytic subunit, for which the three-dimensional structure is known, revealed many homologous residues of probable importance in catalysis and structural folding of ATCases. The significance of homology to E. coli ornithine transcarbamylases was also analyzed. The sequences of the 5' and 3' flanking regions to pyrB encode open reading frames in both cases which overlap with pyrB by eight and six codons, respectively. It is probable that these open reading frames encode other enzymes of a coordinately regulated unit. The sequence 5' to pyrB also encodes an mRNA bearing a pyrimidine-rich sequence followed by a typical sequence for a rho-independent transcription terminator. The presence of these elements and the 5' open reading frame suggest that B. subtilis pyrB, like E. coli pyrBI, is regulated by an attenuation mechanism.