gebhart/patrepeval huggingface dataset:
Virulence of Avian Influenza A Viruses for Squirrel Monkeys (1982)

Ten serologically distinct avian influenza A viruses were administered to squirrel monkeys and hamsters to compare their replication and virulence with those of human influenza A virus, A/Udorn/307/72 (H3N2). In squirrel monkeys, the 10 avian influenza A viruses exhibited a spectrum of replication and virulence. The levels of virus replication and clinical response were closely correlated. Two viruses, A/Mallard/NY/6874/78 (H3N2) and A/Pintail/Alb/121/79 (H7N8), resembled the human virus in their level and duration of replication and in their virulence. At the other end of the spectrum, five avian viruses were restricted by 100- to 10,000-fold in replication in the upper and lower respiratory tract and were clearly attenuated compared with the human influenza virus. In hamsters, the 10 viruses exhibited a spectrum of replication in the nasal turbinates, ranging from viruses that replicated as efficiently as the human virus to those that were 8,000- fold restricted. Since several avian viruses were closely related serologically to human influenza viruses, studies were done to confirm the avian nature of these isolates. Each of the avian viruses plaqued efficiently at 42 degrees C, a restrictive temperature for replication of human influenza A viruses. Avian strains that had replicated either very efficiently or very poorly in squirrel monkeys still grew to high titer in the intestinal tracts of ducks, a tropism characteristic of avian, but not mammalian, influenza viruses. These observations indicate that some avian influenza A viruses grow well and cause disease in a primate host, whereas other avian viruses are very restricted in this host. These findings also provide a basis for determining the gene or genes involved in the restriction of replication that is observed with the attenuated avian viruses. Application of such information may allow the preparation of reassortant viruses derived from a virulent human influenza virus and an attenuated avian virus for possible use in a live attenuated vaccine for prevention of influenza in humans.
Births of normal daughters after MicroSort sperm separation and intrauterine insemination, in-vitro fertilization, or intracytoplasmic sperm injection (1998)

The world's first deliveries of normal babies after use of flow cytometric separated human sperm cells (MicroSort) for preconception gender selection are reported. Offspring were of the desired female gender in 92.9% of the pregnancies. Most of these pregnancies and births were achieved after simple intrauterine insemination.
Discovering Spammers in Social Networks (2021)

As the popularity of the social media increases, as evidenced in Twitter, Facebook and China's Renren, spamming activities also picked up in numbers and variety. On social network sites, spammers often disguise themselves by creating fake accounts and hijacking normal users' accounts for personal gains. Different from the spammers in traditional systems such as SMS and email, spammers in social media behave like normal users and they continue to change their spamming strategies to fool anti spamming systems. However, due to the privacy and resource concerns, many social media websites cannot fully monitor all the contents of users, making many of the previous approaches, such as topology-based and content-classification-based methods, infeasible to use. In this paper, we propose a novel method for spammer detection in social networks that exploits both social activities as well as users' social relations in an innovative and highly scalable manner. The proposed method detects spammers following collective activities based on users' social actions and relations. We have empirically tested our method on data from Renren.com, which is the largest social network in China, and demonstrated that our new method can improve the detection performance significantly.
Transforming Growth Factor β and Atherosclerosis: So Far, So Good for the Protective Cytokine Hypothesis (2003)

The role of the anti-inflammatory cytokine transforming growth factor beta (TGF-beta) in atherosclerosis has been the subject of considerable debate for a decade. In the early 1990s, we postulated that TGF-beta played an important role in maintaining normal vessel wall structure and that loss of this protective effect contributed to the development of atherosclerosis. We termed this the protective cytokine hypothesis. This proposal was slow to gain broad acceptance, however, because at that time there were little data available on the role of TGF-beta during the development of atherosclerosis but much information about its role during trauma-induced neointima formation. Because TGF-beta apparently aggravates neointima formation, both by inhibiting endothelial regeneration and by promoting fibrosis, it was difficult to accept that its presence might ameliorate the superficially similar atherogenesis process. But several recent studies revealed beyond doubt the fact that TGF-beta protects against lipid lesion formation, at least in mouse models of atherosclerosis. Therefore, two important questions remain. First, is the role of TGF-beta in vascular biology similar in humans and in mice? Secondly, how important, compared with defects in thrombosis or lipoprotein metabolism, is the protective role of TGF-beta during atherogenesis?
Nature of the use of adventitious carbon as a binding energy standard (1995)

It has become common practice to employ, as a binding energy reference for x-ray photoelectron spectroscopy studies on nonconductive materials, the C(1s) spectra of the ubiquitous (adventitious) carbon that seems to exhibit an instantaneous presence on all air exposed materials. Despite this commonality, surface scientists, including many practitioners, have expressed substantial concerns about the validity of this approach. A detailed discussion of the method is presented including consideration of the types of materials and the electronic energy states involved, e.g., Fermi edges, vacuum levels, etc., and the couplings that must exist for the referencing method to be correctly applied. A number of other surface environments for which the carbon referencing method may be fallacious are also presented. This leads to a consideration of the electron spectroscopy for chemical analysis results for different types of adventitious species and how the presence of some of these may confuse the use of the method. In this regard, we will also discuss the use of other methods to establish binding energy scales, such as Fermi edge coupling and select doping (e.g., the Au dot approach).
Fucosyl-glycoprotein and precursor pools in HeLa cells (1975)

An enzymatic-radioactive isotope method has been developed for the direct quantitation of L-fucose in amounts as low at 0.5 plus or minus 0.05 nmol. Fucose kinase is used to transfer [32-P]phosphate from ATP to [3-H]fucose. The labeled enzymatic products are then separated electrophoretically and the amount and specific activity of the fucose are determined from the known specific activity of the phosphate donor. This assay has been used to measure the GDP-L-fucose and macromolecualar fucose in HeLa cells after extraction and purification of the sugar. It has been determined there are 0.5 nmol of GDP-L-fucose in 10-7 cells with a nine- to tenfold dilution of specific activity in converting L-[3-H] fucose to GDP-L-[3-H]fucose. After 2 to 3 days of labeling, the GDP-L-[3-H]fucose pool is essentially at equilibrium with the macromolecular pool, and hence it can be concluded that the dilution of label is due to a nine- to tenfold contribution to GDP-L-fucose from an endogenous source, as compared to exogenously supplied fucose. The fucosyl-glycoprotein pool has been shown to be much larger containing 6 to 8 nmol of fucose in 10-7 cells. It has further been shown that GDP-fucose is the only soluble fucose intermediate present in significant amount.
Electronic effect of the tricyanomethyl group by carbon-13 and fluorine-19 NMR: nature of aryl fluorine-19 NMR polar field effects in the benzene and naphthalene ring systems (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectronic effect of the tricyanomethyl group by carbon-13 and fluorine-19 NMR: nature of aryl fluorine-19 NMR polar field effects in the benzene and naphthalene ring systemsWilliam Adcock and Douglas P. CoxCite this: J. Org. Chem. 1979, 44, 17, 3004–3017Publication Date (Print):August 1, 1979Publication History Published online1 May 2002Published inissue 1 August 1979https://pubs.acs.org/doi/10.1021/jo01331a011https://doi.org/10.1021/jo01331a011research-articleACS PublicationsRequest reuse permissionsArticle Views183Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Novel projection engine with dual paraboloid reflector and polarization recovery systems (2004)

Projection engine designs are mostly based on discrete optical components including color filters, mirrors, relay lenses, prisms, etc., which tends to be expensive and increase the space requirement. With the advancement of light pipe based illumination system, e.g. the Wavien patented dual paraboloid reflector system, and light-pipe based polarization recovery system, it would be advantage to design a light-pipe based projection engine for a complete light-pipe based system for low cost and space saving applications. In this paper, a totally light-pipe based projection engine is described. It consists of an etendue efficiency illumination system using the dual paraboloid reflector system with a lensed tapered light pipe as the output. The output is then directed into a light-pipe based polarization recovery system such that the output is polarized. The polarized light is then separated into its individual RGB colors, and is directed into the corresponding HTPS imager chips separately by the use of light pipes, prism, and beam splitters. The outputs from the imager chips are then recombined by an X-cube and projected onto the screen. This light-pipe based system uses low cost optical components and take up much less space than the traditional projection system.
The 5'-flanking region of the human CGL-1/granzyme B gene targets expression of a reporter gene to activated T-lymphocytes in transgenic mice. (1991)

The human CSP-B/CGL-1 gene is the homologue of the mouse granzyme B/CCPI gene and encodes a cytotoxic T-lymphocyte-specific serine protease. We have used regulatory sequences upstream from the CSP-B gene to drive human growth hormone gene expression in transgenic mice. Eleven founder mice were screened for transgene expression in activated T-cells. Expression was detected in 10 mice; levels of expression were integration site-dependent. The transgene was not expressed in resting lymphocytes but could be activated by treatment with concanavalin A or interleukin-2, indicating that CSP-B regulatory sequences are responsive to signals originating at either the T-cell receptor or the interleukin-2 receptor. Transgene expression was detected at the whole organ level only in lymph nodes and small intestine, where endogenous mouse CCPI mRNA was also present. The time course of transgene activation in T-lymphocytes was similar to that of the mouse CCPI gene. No differences in levels of expression of the transgene were observed in activated lymphocyte populations that had been depleted of either CD4+ or CD8+ cells; in contrast, the mouse CCPI gene was expressed primarily in CD8+ cells. Six CD4+ T-cell clones with Th0, Th1, or Th2 phenotypes were generated from a transgenic animal. All clones expressed moderate to high levels of the transgene, but only three clones expressed mouse CCPI, indicating that the transgene is disregulated in CD4+ T-cell subsets. The CSP-B regulatory unit represents a novel reagent for targeting gene expression to activated T-lymphocytes.
Effects of Cation Binding on the Conformation of Calsequestrin and the High Affinity Calcium-binding Protein of Sarcoplasmic Reticulum (1974)

Abstract The circular dichroism spectra of calsequestrin and the high affinity calcium-binding protein from rabbit skeletal muscle sarcoplasmic reticulum have been recorded in the presence and absence of Ca2+ and other cations. Calsequestrin Forms I and II show decreases in negativity upon Ca2+ binding from -12 to -6 x 103 and -20 to -10 x 103 deg cm2 dmole-1, respectively, at 200 nm, and increases from -5 to -6 x 103 and -7 to -10 x 103 deg cm2 dmole-1 at 220 nm. Above 250 nm there is little optical activity in the absence of cations, but both forms of calsequestrin show a complex signal with maximum of about 90 deg cm2 dmole-1 between 270 and 285 nm in their presence. The changes in circular dichroism are interpreted as reflecting a large conformational change in the protein upon cation binding. Absorption difference spectra in the 320 to 240 nm range confirm that calcium binding induces perturbation in the absorption bands of tryosine, tryptophan, and phenylalanine, probably as a result of greater folding. The circular dichroism and absorption spectra of the high affinity calcium-binding protein do not change upon binding calcium. This protein, even in the absence of calcium, has intense circular dichroism activity in the aromatic region, with a maximum of 250 deg cm2 dmole-1 at about 280 nm.
The current options in the management of actinic keratosis. (2009)

Actinic keratosis is a precursor for squamous cell skin cancer. Hence, it is important to treat. For single actinic keratoses, cryo surgery is recommended. For widespread actinic keratoses, imiquimod, photodynamic therapy (PDT) or 5-FU may be used. ''One-shot'' treatments are advocated as prolonged treatment over several weeks probably diminishes patient compliance. Consequently, cryo surgery for single actinic keratoses and PDT for widespread actinic keratoses should primarily be recommended.
A unique, efficient, implantable, electromechanical, total artificial heart. (1992)

A completely implantable, one piece electromechanical total artificial heart (TAH) intended for permanent human use was developed. It consisted of left and right conically shaped pusher-plate blood pumps sandwiching a thin centerpiece with a compact, efficient electromechanical actuator. The actuator consisted of a direct current brushless motor; a planetary roller screw fit the space between the two conically shaped pusher-plates. The rotational motion of the motor was converted to the rectilinear motion of the rollerscrew to displace the left and right pusher-plates in the left master alternate mode. The diameter of the assembled TAH was 97 mm, with a central thickness of 82 mm. The overall weight was 620 g, with a displaced volume of 510 ml. The pump provided flows of 3-8 L/min with a preload of 1-15 mmHg against an afterload of 100 mmHg. The net efficiency ranged from 15% to 18%. This model showed good fit in the pericardial space of heart transplant recipients (body weight, 77 kg).
The impact of acute stress on hormones and cytokines and how their recovery is affected by music-evoked positive mood (2016)

Stress and recovery from stress significantly affect interactions between the central nervous system, endocrine pathways, and the immune system. However, the influence of acute stress on circulating immune-endocrine mediators in humans is not well known. Using a double-blind, randomized study design, we administered a CO2 stress test to n = 143 participants to identify the effects of acute stress, and recovery from stress, on serum levels of several mediators with immune function (IL-6, TNF-α, leptin, and somatostatin), as well as on noradrenaline, and two hypothalamic-pituitary-adrenal axis hormones (ACTH and cortisol). Moreover, during a 1 h-recovery period, we repeatedly measured these serum parameters, and administered an auditory mood-induction protocol with positive music and a neutral control stimulus. The acute stress elicited increases in noradrenaline, ACTH, cortisol, IL-6, and leptin levels. Noradrenaline and ACTH exhibited the fastest and strongest stress responses, followed by cortisol, IL-6 and leptin. The music intervention was associated with more positive mood, and stronger cortisol responses to the acute stressor in the music group. Our data show that acute (CO2) stress affects endocrine, immune and metabolic functions in humans, and they show that mood plays a causal role in the modulation of responses to acute stress.
Bothnia dystrophy is caused by domino-like rearrangements in cellular retinaldehyde-binding protein mutant R234W (2009)

Cellular retinaldehyde-binding protein (CRALBP) is essential for mammalian vision by routing 11-cis-retinoids for the conversion of photobleached opsin molecules into photosensitive visual pigments. The arginine-to-tryptophan missense mutation in position 234 (R234W) in the human gene RLBP1 encoding CRALBP compromises visual pigment regeneration and is associated with Bothnia dystrophy. Here we report the crystal structures of both wild-type human CRALBP and of its mutant R234W as binary complexes complemented with the endogenous ligand 11-cis-retinal, at 3.0 and 1.7 A resolution, respectively. Our structural model of wild-type CRALBP locates R234 to a positively charged cleft at a distance of 15 A from the hydrophobic core sequestering 11-cis-retinal. The R234W structural model reveals burial of W234 and loss of dianion-binding interactions within the cleft with physiological implications for membrane docking. The burial of W234 is accompanied by a cascade of side-chain flips that effect the intrusion of the side-chain of I238 into the ligand-binding cavity. As consequence of the intrusion, R234W displays 5-fold increased resistance to light-induced photoisomerization relative to wild-type CRALBP, indicating tighter binding to 11-cis-retinal. Overall, our results reveal an unanticipated domino-like structural transition causing Bothnia-type retinal dystrophy by the impaired release of 11-cis-retinal from R234W.
Soft-Tissue Blood Flow Following Reamed Versus Unreamed Locked Intramedullary Nailing: A Fractured Sheep Tibia Model (1996)

A study was performed to compare the effects of reamed versus unreamed locked intramedullary nailing on soft-tissue blood flow in a fractured sheep tibia model. After creation of a standardized short spiral fracture, each tibia was stabilized by insertion of a locked intramedullary nail. Ten animals were randomized into two groups: one that had reaming prior to nail insertion and one that did not. Blood flow was measured in real time, using laser Doppler flowmetry. Skin and muscle perfusion measurements were made at three locations (proximal diaphysis, fracture site, and distal diaphysis) and at five time intervals (postfracture; postnail insertion; and at 2-week, 6-week, and 12-week follow-ups). All animals were sacrificed at 12 weeks postoperatively. Muscle blood flow remained elevated longer in the reamed group than in the unreamed group, following completion of the procedure. Muscle perfusion was significantly greater in the reamed group than in the unreamed group (p < 0.033) and was significantly greater than skin perfusion at 2 weeks (p < 0.024). There was no difference in skin perfusion between the reamed and unreamed groups at any time interval. The study demonstrates that muscle perfusion is altered following reamed and unreamed intramedullary nailing. The findings demonstrate the change in blood flow in the surrounding soft-tissue envelope during the process of revascularization, following devascularization of a segment of tibial cortex by intramedullary nailing.
Generating Shorter Bases for Hard Random Lattices (2009)

We revisit the problem of generating a ``hard'' random lattice together with a basis of relatively short vectors. This problem has gained in importance lately due to new cryptographic schemes that use such a procedure for generating public/secret key pairs. In these applications, a shorter basis directly corresponds to milder underlying complexity assumptions and smaller key sizes. The contributions of this work are twofold. First, using the \emph{Hermite normal form} as an organizing principle, we simplify and generalize an approach due to Ajtai (ICALP 1999). Second, we improve the construction and its analysis in several ways, most notably by tightening the length of the output basis essentially to the optimum value.
Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristics of C3/C4 binding proteins. (1987)

10 overlapping CR1 cDNA clones that span 5.5 kb were isolated from a tonsillar library and sequenced in whole or in part. A single long open reading frame beginning at the 5' end of the clones and extending 4.7 kb downstream to a stop codon was identified. This sequence represents approximately 80% of the estimated 6 kb of coding sequence for the F allotype of CR1. Three tandem, direct, long homologous repeats (LHRs) of 450 amino acids were identified. Analysis of the sequences of tryptic peptides provided evidence for a fourth LHR in the F allotype of CR1. Amino acid identity between the LHRs ranged from 70% between the first and third repeats to 99% between the NH2-terminal 250 amino acids of the first and second repeats. Each LHR comprises seven short consensus repeats (SCRs) of 60-70 amino acids that resemble the SCRs of other C3/C4 binding proteins, such as complement receptor type 2, factors B and H, C4 binding protein, and C2. Two additional SCRs join the LHRs to a single membrane-spanning domain of 25 amino acids; thus, the F allotype of CR1 probably contains at least 30 SCRs, 23 of which have been sequenced. Each SCR is predicted to form a triple loop structure in which the four conserved half-cystines form disulfide linkages. The linear alignment of 30 SCRs as a semi-rigid structure would extend 1,140A from the plasma membrane and might facilitate the interaction of CR1 with C3b and C4b located within the interstices of immune complexes and microbial cell walls. The COOH-terminal cytoplasmic domain of 43 residues contains a six-amino-acid sequence that is homologous to the sequence in the epidermal growth factor receptor that is phosphorylated by protein kinase C.
Randomized Evaluation of the Effects of Filter-Based Distal Protection on Myocardial Perfusion and Infarct Size After Primary Percutaneous Catheter Intervention in Myocardial Infarction With and Without ST-Segment Elevation (2005)

The filter wire as an adjunct to primary percutaneous catheter intervention in myocardial infarction with and without ST-segment elevation did not improve reperfusion or reduce infarct size.
COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer (2015)

This study substantiates the influence of altered O-glycan (Tn/STn) expression on oncogenic properties in pancreatic cancer and identifies O-GalNAc modified Nucleolin as novel prognostic marker.