gebhart/patrepeval huggingface dataset:
Beta-amyloid (25-35) peptide and IFN-γ synergistically induce the production of the chemotactic cytokine MCP-1/JE in monocytes and microglial cells (1996)

The molecular mechanisms involved in the development of senile plaques characteristic of aging and Alzheimer's disease are poorly understood. In this study, we examined whether human monocytes and murine microglial cells stimulated with the active fragment of amyloid beta-protein (Abeta(25-35)) express the monocyte chemotactic protein-1 (MCP-1)/JE. We show that upon incubation with Abeta(25-35), human monocytes accumulate MCP-1 mRNA and produce significant amounts of MCP-1. The effect of Abeta(25-35) on MCP-1 secretion was neither mimicked by a scrambled analogue nor affected by polymyxin B sulfate, even though the latter almost completely abolished the effect of LPS on MCP-1 expression. Murine microglial cells stimulated with Abeta(25-35) also expressed high levels of JE mRNA (the murine counterpart of MCP-1) and released bioactive chemotactic factors. In addition, we report that IFN-gamma significantly synergizes with Abeta(25-35) either in human monocytes or in murine microglial cells, and that Abeta(25-35) plus/minus IFN-gamma-mediated early induction of MCP-1 mRNA does not require new protein synthesis. Finally, we provide evidence that the Abeta(25-35)- and Abeta plus IFN-gamma-induced production of MCP-1 is, in large part, mediated in an autocrine fashion by endogenous TNF-alpha. Taken together, our findings uncover another novel biologic action of Abeta(25-35) and might help in better understanding the mechanisms underlying mononuclear phagocyte recruitment and activation into amyloid deposits.
Human skin fibroblast collagenase inhibitor. Purification and biochemical characterization. (1983)

Human skin fibroblasts maintained in cell culture secrete a collagenase inhibitor which has been purified to homogeneity from serum-containing culture medium using a combination of salt precipitation, and ion-exchange, phenylboronate-agarose, gel filtration, and high pressure liquid chromatography. The pure inhibitor retained full activity and exhibited a 1:1 molar inhibition of collagenase. A single band of Mr = 28,500 was seen on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amino acid composition was remarkable for the presence of 24 half-cystine residues. No free sulfhydryls were present and the resultant 12 disulfide bridges may account for the remarkable stability of this protein to extremes of pH, temperature, pressure, as well as to denaturing agents. A total of 13 hexose residues/molecule were found. NH2-terminal sequence analysis revealed the presence of a single polypeptide chain and the first 23 residues were identified. A monospecific antibody was produced which abolished the functional activity of the inhibitor. Fibroblast inhibitor was found to migrate with the beta-globulins by immunoelectrophoresis. A chromatographically and immunologically identical collagenase inhibitor was partially purified from human serum, suggesting the possibility that the fibroblast-derived inhibitor and the previously reported serum beta 1-anticollagenase (Woolley, D. E., Roberts, D. R., and Evanson, J. M., (1975) Biochem. Biophys. Res. Commun. 66, 747-754) are similar, if not identical, proteins. The fibroblast collagenase inhibitor was found to be clearly distinct from other collagenase inhibitors of leukocyte and serum origin.
MR Imaging Predictors of Molecular Profile and Survival: Multi-institutional Study of the TCGA Glioblastoma Data Set (2013)

This analysis demonstrates a method for consistent image feature annotation capable of reproducibly characterizing brain tumors; this study shows that radiologists' estimations of macroscopic imaging features can be combined with genetic alterations and gene expression subtypes to provide deeper insight to the underlying biologic properties of GBM subsets.
Time-Dependent Inhibition of recA Protein-Catalyzed ATP Hydrolysis by ATPγS:  Evidence for a Rate-Determining Isomerization of the recA−ssDNA Complex (1997)

The ATP analog ATPgammaS is a competitive inhibitor of the recA protein-catalyzed ssDNA-dependent ATP hydrolysis reaction. The degree of inhibition by ATPgammaS, however, changes in a time-dependent manner and is consistent with a two step binding mechanism. In the first step, ATPgammaS binds to the recA-ssDNA complex in a rapid equilibrium step (KD = 50 microM). This initial binding step is followed by an isomerization of the recA-ssDNA-ATPgammaS complex to a new conformational state in which ATPgammaS is bound with a significantly higher affinity (overall K(D) = 0.3 microM). This isomerization is followed by the slow hydrolysis of ATPgammaS to ADP and thiophosphate (0.01 min(-1)). The first-order rate constant for the ATPgammaS-mediated isomerization step (20 min(-1)), although significantly greater than the rate of ATPgammaS hydrolysis, is identical to the steady-state rate constant for the recA protein-catalyzed ATP hydrolysis reaction. These results are consistent with a kinetic model in which an ATP-mediated isomerization of the recA-ssDNA complex represents the rate-determining step on the recA protein-catalyzed ssDNA-dependent ATP hydrolysis reaction pathway.
An unusual Donath‐Landsteiner antibody detectable at 37°C by the antiglobulin test (1985)

A 14-year-old boy with no history of transfusion experienced a hemolytic crisis attributed to paroxysmal cold hemoglobinuria. The Donath-Landsteiner test was positive. The IgG autoantibody had anti-P specificity but was unique in that it also reacted strongly by indirect antiglobulin test at 37 degrees C.
Dielectric dependence of EL brightness of powdered phosphor and particle size distribution (1981)

The particle size distribution of a phosphor material plays an important role in determining the effect of the dielectric constant of a dispersive medium on the electroluminescent brightness. The effect of the dielectric constant of the dispersive medium is to modify the electric field inside the phosphor without affecting the electrical characteristics. In the present investigation a semi-empirical calculation is made on how the brightness of a phosphor of given particle size distribution depends on the dielectric constant of the dispersive medium. An experimental measurement of EL brightness for ZnS: Cu, Cl phosphor in various dielectric media is described in support of the theoretical results. A probable explanation of the behaviour is given. Die Verteilung der Partikelgroße eines Leuchtmaterials spielt eine wesentliche Rolle bei der Be-stimmung des Einflusses der Dielektrizititskonstante des dispersiven Mediums auf die Elektro-lumineszenzhelligkeit. Der Einfluß der Dielektrizitütskonstante des dispersiven Mediums besteht in der Modifizierung des elektrischen Feldes innerhalb des Phosphors ohne die elektrische Charak-teristik zu ündern. Es werden halbempirische Rechnungen darüber durchgeführt, wie die Hellig-keit eines Phosphors mit vorgegebener Verteilung der Partikelgroße von der Dielektrizitütskonstante des dispersiven Mediums abhüngt. Zur Bestätigung der theoretischen Ergebnisse werden experimentelle Messungen der EL-Helligkeit fur ein ZnS: Cu, CI-Phosphor in verschiedenen di-elektrischen Medien durchgeführt. Es wird eine wahrscheinliche Erklärung des Verhaltens gegeben.
Phase II Trial of Sorafenib in Advanced Thyroid Cancer (2008)

Sorafenib has clinically relevant antitumor activity in patients with metastatic, iodine-refractory thyroid carcinoma, with an overall clinical benefit rate (partial response + stable disease) of 77%, median PFS of 79 weeks, and an overall acceptable safety profile. These results represent a significant advance over chemotherapy in both response rate and PFS and support further investigation of this agent in these patients.
Effects of Recombinant Apolipoprotein A-I Milano on Aortic Atherosclerosis in Apolipoprotein E–Deficient Mice (1998)

Recombinant A-I(Milano)/PC prevented progression of aortic atherosclerosis and reduced lipid and macrophage content of plaques in apo E-deficient mice despite severe hypercholesterolemia. Thus, A-I(Milano)/PC may have a role in inhibiting progression and promoting stabilization of atherosclerosis.
Circulating plasma levels of nucleosomes in patients with systemic lupus erythematosus. Correlation with serum antinucleosome antibody titers and absence of clear association with disease activity (1997)

Nucleosome plasma levels may be normal or increased in SLE, and found in patients with active or inactive SLE. Longitudinal studies are needed to further establish whether high levels of circulating nucleosomes may predict the occurrence of an SLE flare.
STAT3 mediates hepatic hepcidin expression and its inflammatory stimulation (2006)

Hepcidin is a key iron-regulatory hormone produced by the liver. Inappropriately low hepcidin levels cause iron overload, while increased hepcidin expression plays an important role in the anemia of inflammation (AI) by restricting intestinal iron absorption and macrophage iron release. Its expression is modulated in response to body iron stores, hypoxia, and inflammatory and infectious stimuli involving at least in part cytokines secreted by macrophages. In this study we established and characterized IL6-mediated hepcidin activation in the human liver cell line Huh7. We show that the proximal 165 bp of the hepcidin promoter is critical for hepcidin activation in response to exogenously administered IL6 or to conditioned medium from the monocyte/macrophage cell line THP-1. Importantly, we show that hepcidin activation by these stimuli requires a STAT3 binding motif located at position -64/-72 of the promoter. The same STAT binding site is also required for high basal-level hepcidin mRNA expression under control culture conditions, and siRNA-mediated RNA knockdown of STAT3 strongly reduces hepcidin mRNA expression. These results identify a missing link in the acute-phase activation of hepcidin and establish STAT3 as a key effector of baseline hepcidin expression and during inflammatory conditions.
Development of an efficient automated hyperspectral processing system using embedded computing (2012)

Automated hyperspectral image processing enables rapid detection and identification of important military targets from hyperspectral surveillance and reconnaissance images. The majority of this processing is done using ground-based CPUs on hyperspectral data after it has been manually exfiltrated from the mobile sensor platform. However, by utilizing high-performance, on-board processing hardware, the data can be immediately processed, and the exploitation results can be distributed over a low-bandwidth downlink, allowing rapid responses to situations as they unfold. Additionally, transitioning to higher-performance and more-compact processing architectures such as GPUs, DSPs, and FPGAs will allow the size, weight, and power (SWaP) demands of the system to be reduced. This will allow the next generation of hyperspectral imaging and processing systems to be deployed on a much wider range of smaller manned and unmanned vehicles. In this paper, we present results on the development of an automated, near-real-time hyperspectral processing system using a commercially available NVIDIA&reg; Telsa&trade; GPU. The processing chain utilizes GPU-optimized implementations of well-known atmospheric-correction, anomaly-detection, and target-detection algorithms in order to identify targetmaterial spectra from a hyperspectral image. We demonstrate that the system can return target-detection results for HYDICE data with 308&times;1280 pixels and 145 bands against 30 target spectra in less than four seconds.
Correlation of the immunohistochemical reactivity of mucin peptide cores MUC1 and MUC2 with the histopathological subtype and prognosis of gastric carcinomas (1998)

The expression of MUC1 and MUC2 mucin peptide core antigens in gastric carcinomas was studied by immunohistochemistry to determine correlations with TNM stage and histo-pathological classifications as well as a possible prognostic impact. Paraffin-embedded specimens from 128 gastric carcinomas with a minimal follow-up of 5 years were immunostained. In addition to a polyclonal antiserum generated against polymorphic epithelial mucin (MUC1) from human milk, 2 monoclonal antibodies (MAbs), HMFG2 (anti-MUC1) and 4FI (anti-MUC2), were applied. Reactivity of carcinomas was correlated with the classifications of the UICC (TNM), WHO and Laurén. Correlations with overall survival were analyzed using the Kaplan and Meier product limit method. MUC1 immunoreactivity was associated with an advanced pTNM stage. The demonstration of both mucin species (MUC1, MUC2) displayed a statistically significant correlation with tubular/papillary vs. signet-ring cell differentiation as well as with intestinal-type vs. diffuse-type of tumor growth according to Laurén. In particular, MUC2 was only rarely detectable in signet-ring cell and diffuse-type tumors. MUC1 correlated with poor prognosis in all cases and the subgroup of stage I tumors. According to the histopathological classifications, a similar result was observed in signet-ring cell and diffuse-type carcinomas. In contrast, MUC2 reactivity was associated with a favourable prognosis of intestinal-type carcinomas. In the non-neoplastic gastric mucosa, both peptide cores were recognized in the superficial epithelium, whereas parietal cells contained only MUC1, and intestinal metaplasia almost exclusively MUC2 antigens. We conclude that the mucin peptide core antigens are suitable markers for the tubule-rich gastric carcinomas, which may in part be derived from intestinal metaplasia. In addition, MUC1 may exert a prognostic relevance and appears to be involved in the progression of diffuse-type tumors.
Evaluating instruction set extensions for fast arithmetic on binary finite fields x (2004)

Binary finite fields GF(2/sup n/) are very commonly used in cryptography, particularly in public-key algorithms such as elliptic curve cryptography (ECC). On word-oriented programmable processors, field elements are generally represented as polynomials with coefficients from [0, 1]. Key arithmetic operations on these polynomials, such as squaring and multiplication, are not supported by integer-oriented processor architectures. Instead, these are implemented in software, causing a very large fraction of the cryptography execution time to be dominated by a few elementary operations. For example, more than 90% of the execution time of 163-bit ECC may be consumed by two simple field operations: squaring and multiplication. A few processor architectures have been proposed recently that include instructions for binary field arithmetic. However, these have only considered processors with small wordsizes and in-order, single-issue execution. The first contribution of this paper is to validate these new arithmetic instructions for processors with wider wordsizes and multiple-issue (e.g. superscalar) execution. We also consider the effects of varying the number of functional units and load/store pipes. We demonstrate that the combination of microarchitecture and new instructions provides speedups up to 22.4x for ECC point multiplication. Second, we show that if a bit-level reverse instruction is included in the instruction set, the size of the multiplier can be reduced by half without significant performance degradation. Third, we compare the benefits of superscalar execution with wordsize scaling. The latter has been used in recent processor architectures such as PLX and PAX as a new way to extract parallelism. We show that 2x wordsize scaling provides 70% better performance than 2-way superscalar execution. Finally, we suggest a low-cost method, which we call multi-word result execution, to realize some of the benefits of wordsize scaling in existing processors with fixed wordsizes.
The Hydroxylation of Unsaturated Substances. IV. The Catalytic Hydroxylation of Unsaturated Hydrocarbons1 (1937)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Hydroxylation of Unsaturated Substances. IV. The Catalytic Hydroxylation of Unsaturated Hydrocarbons1Nicholas A. Milas and Sidney SussmanCite this: J. Am. Chem. Soc. 1937, 59, 11, 2345–2347Publication Date (Print):November 1, 1937Publication History Published online1 May 2002Published inissue 1 November 1937https://pubs.acs.org/doi/10.1021/ja01290a073https://doi.org/10.1021/ja01290a073research-articleACS PublicationsRequest reuse permissionsArticle Views392Altmetric-Citations64LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A decision tree-based approach to dynamic pointcut evaluation (2008)

Constructs of dynamic nature, e.g., history-based pointcuts and control-flow based pointcuts, have received significant attention in recent aspect-oriented literature. A variety of compelling use cases are presented that motivate the need for efficiently supporting such constructs in language implementations. The key challenge in implementing dynamic constructs is to efficiently support runtime adaptation of the set of intercepted join points at a fine-grained level. This translates to two high-level requirements. First, since the set of intercepted join points may change, such implementations must provide an efficient method to determine this set membership, i.e., whether the currently executing join point needs to be intercepted. Second, the frequency with which such set membership needs to be determined must be minimized. In previous work, Dyer and Rajan proposed a dedicated caching mechanism to address the second requirement. In this work, we propose a mechanism to address the first requirement. This requirement translates to efficiently evaluating whether a join point is intercepted by a set of pointcut expressions. In the worst case, at every join point there may be the need to determine whether it is intercepted. Therefore, even modest savings in such mechanisms is likely to translate to significant savings in the long run.
Effect of Desipramine on a Glycoprotein Sialyltransferase Activity in C6 Cultured Glioma Cells (1990)

The tricyclic antidepressant desipramine, when added to culture medium, gave rise in C6 rat glioma cells to a decrease of the activity of the enzyme asialofetuin sialyltransferase. The inhibition was dose and time dependent and was observed in both multiplying cells and cells blocked with 2 mM thymidine or depletion of amino acids. This inhibition was rather specific to the sialyltransferase, as under the conditions where this enzyme was inhibited up to 70%, other enzymes such as dolichol phosphate mannose synthetase, glutamine synthetase, and glycerol phosphate dehydrogenase remained unaffected. This inhibition was not reversed after removal of desipramine from the medium and was not observed by direct addition of desipramine to the sialyltransferase incubation assay. Under the same conditions, W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide], which is known to be a potent calmodulin antagonist and an inhibitor of calmodulin-dependent kinases, gave the same concentration-dependent inhibition profile of sialyltransferase as desipramine, whereas H-7 [1-(5-isoquinolinylsulfonyl)-2-methylpiperazine], which is an inhibitor of protein kinase C and cyclic nucleotide-dependent kinases, had no effect. So, it is suggested that desipramine inhibits the sialyltransferase activity in C6 glioma cells through a calmodulin-dependent system.
Identification and map position of YAC clones comprising one‐third of the Arabidopsis genome. (1991)

YAC clones corresponding to 125 Arabidopsis thaliana RFLP markers have been identified. At least one YAC clone has been isolated for each of the RFLP markers tested. Based on CHEF gel analysis of 196 clones, the mean insert size of the available Arabidopsis YAC libraries is approximately 160 kb. The YACs of known genetic map location encompass about 30% of the Arabidopsis genome. The results presented here represent a first step towards assembly of an overlapping YAC library of the A. thaliana genome.
Total synthesis of (.+-.)-ginkgolide B (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of (.+-.)-ginkgolide BElias J. Corey, Myung Chol. Kang, Manoj C. Desai, Arun K. Ghosh, and Ioannis N. HoupisCite this: J. Am. Chem. Soc. 1988, 110, 2, 649–651Publication Date (Print):January 1, 1988Publication History Published online1 May 2002Published inissue 1 January 1988https://pubs.acs.org/doi/10.1021/ja00210a083https://doi.org/10.1021/ja00210a083research-articleACS PublicationsRequest reuse permissionsArticle Views7951Altmetric-Citations171LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Evaluation of Bottlenecks in the Late Stages of Protein Secretion in Bacillus subtilis (1999)

Despite a high capacity for secretion of homologous proteins, the secretion of heterologous proteins by Bacillus subtilis is frequently inefficient. In the present studies, we have investigated and compared bottlenecks in the secretion of four heterologous proteins: Bacillus lichenifomis alpha-amylase (AmyL), Escherichia coli TEM beta-lactamase (Bla), human pancreatic alpha-amylase (HPA), and a lysozyme-specific single-chain antibody. The same expression and secretion signals were used for all four of these proteins. Notably, all identified bottlenecks relate to late stages in secretion, following translocation of the preproteins across the cytoplasmic membrane. These bottlenecks include processing by signal peptidase, passage through the cell wall, and degradation in the wall and growth medium. Strikingly, all translocated HPA was misfolded, its stability depending on the formation of disulfide bonds. This suggests that the disulfide bond oxidoreductases of B. subtilis cannot form the disulfide bonds in HPA correctly. As the secretion bottlenecks differed for each heterologous protein tested, it is anticipated that the efficient secretion of particular groups of heterologous proteins with the same secretion bottlenecks will require the engineering of specifically optimized host strains.