gebhart/patrepeval huggingface dataset:
PPAR γ activators induce growth arrest and process extension in B12 oligodendrocyte‐like cells and terminal differentiation of cultured oligodendrocytes (2003)

Peroxisome proliferator-activated receptors (PPARs) are key transcription factors in the control of lipid homeostasis and cell differentiation, but little is known about their function in oligodendrocytes, the major lipid-synthesizing cells in the central nervous system (CNS). Using the B12 oligodendrocyte-like cell line and rat spinal cord-derived oligodendrocytes, we evaluated the importance of PPARgamma in the maturation process of these cells. B12 cells express all PPAR isoforms (alpha, beta/delta, and gamma), as assessed by RT-PCR, Western-blot, and transactivation assays. B12 cells respond specifically to PPARgamma agonists by arresting cell proliferation and extending cell processes, events that are blocked by the PPARgamma antagonist GW9662. In addition, alkyl-dihydroxyacetone phosphate synthase (ADAPS), a key peroxisomal enzyme involved in the synthesis of myelin-rich lipid plasmalogens, is increased in PPARgamma agonist-treated B12 cells. In contrast with B12 cells, both immature and mature isolated spinal cord oligodendrocytes presented a high and similar expression level of ADAPS, as assessed by immunocytochemistry. However, as in B12 cells, isolated spinal cord oligodendrocytes were also found to respond specifically to PPARgamma agonists with a four-fold increase in the number of mature cells. Our data suggest a relevant role for PPARgamma in oligodendrocyte lipid metabolism and differentiation.
Effect of Cellsize Reduction on Polyurethane Foam Physical Properties (1994)

Polymer distribution in rigid PU foams is a complex, position de pendent parameter which can be described in terms of: 1) the overall density and density distribution, 2) the average cellsize and cellsize distribution, 3) cell elonga tion and orientation and 4) the material distribution between struts and windows within the individual cells. Considering that a typical foam contains only 2-3 volume % of polymer, it is not surprising that any variation in the way this material is distributed will have a large impact on most of the physical properties of the foam. In the present paper, we evaluate what effects can be expected from changes in cellsize and/or density on the polymer distribution in foams and consequently on foam physical properties like compressive strength, initial thermal conductivity, and thermal conductivity aging. These results are obtained using a blend of modelling techniques and experimental data. Generally speaking, in PU foams a reduction in the average cellsize will also cause an increase in the amount of material which is present in the cell windows versus the cell struts. It is observed that in most systems, the window thickness remains relatively unaffected by cell size reduction. Also, for a given density, there exists a cell size at which the cells will no longer be closed simply because there is not enough material available to ensure film stability. Since the cell windows represent most of the barrier to diffusion, the rate of aging will be reduced dramatically by this shift in the material distribution as long as the cells are still closed. A reduction in cellsize will, in general, also cause a reduction in the initial thermal conductivity, mostly because of the reduction in the thermal radiation component to foam thermal conductivity. There exists a region, however, where this effect is reversed. Further reductions in cellsize will cause an increase in thermal conductivity rather than a decrease. The impact of cellsize reduction on foam compressive strength is usually negative, again because of the concomitant change in polymer distribution.
Family history and the risk of prostate cancer (1990)

A case-control study was performed to estimate the relative risk of developing prostate cancer for men with a positive family history. Extensive cancer pedigrees were obtained on 691 men with prostate cancer and 640 spouse controls. Fifteen percent of the cases but only 8% of the controls had a father or brother affected with prostate cancer (P less than .001). Men with a father or brother affected were twice as likely to develop prostate cancer as men with no relatives affected. In addition, there was a trend of increasing risk with increasing number of affected family members such that men with two or three first degree relatives affected had a five and 11-fold increased risk of developing prostate cancer. Recognizing that 9-10% of U.S. men will develop prostate cancer in their lifetime, men with a family history of prostate cancer should be advised of their significantly increased prostate cancer risk and should undergo appropriate screening measures for this disease.
Ring transformations of heterocycles. Part 1. Transformation of 4‐amino‐Δ2‐1,2,4‐oxadiazolines into 1,3,4‐oxadiazoles (1991)

Abstract 3‐Aryl‐4‐amino‐δ 2 ‐1,2,4‐oxadiazolines 3 and their N ‐chloroacetyl derivatives 4 , upon treatment with chloroacetic anhydride in refluxing toluene, afford 2‐chloromethyl‐5‐aryl‐1,3,4‐oxadiazoles 5 , suggesting the conversion sequence 3 → 4 → 5 . The generality of the new ring transformation 4 → 5 is supported similar conversion of other 4‐(acylamino)‐1,2,4‐oxadiazolines 8 to 1,3,4‐oxadiazoles 9 .
Workshop on network-I/O convergence (2003)

This is a summary of the NICELI workshop, based on scribe reports written by Olaf Maennel and Vinay Aggarwal, and edited by Jeffrey Mogul and Allyn Romanow with help from the NICELI attendees. The workshop was held in conjunction with SIGCOMM 2003 on 27 August 2003 in Karlsruhe, Germany.Papers and presentations from the workshop are available on the Web at http://www.acm.org/sigs/sigcomm/sigcomm2003/workshop/niceli/Note-taking during an active discussion is a fallible process, so these notes may contain errors. We have encouraged participants to help us find these errors. Corrections made after this article goes to press may be found at the NICELI Web site.
Identification of Quantitative Trait Loci Controlling Days to Flowering and Plant Height in Two Near Isogenic Lines of Maize (1993)

The number of days from planting to flowering is a trait of interest to maize ( Zea mays L.) breeders for its importance in selecting appropriate hybrid parents, and for its role in the utilization of unadapted germplasm. Using molecular marker technology, we were able to identify quantitative trait loci (QTLs) controlling days to flowering and two correlated traits, plant height and total leaf number, in two near isogenic lines (NILs). NC264 and B73G are shorter, earlier versions of SC76 and B73 respectively, developed by introgressing Gaspé Flint and selecting for early flowering through repeated backcrosses. The NILs were screened for introgressed chromosomal regions with restriction fragment length polymorphisms (RFLPs). Seven introgressed regions were identified in NC264 and two in B73G, with a specific chromosome 8 region maintained in both NILs. The introgressed regions were tested for their effect on flowering date and plant height in segregating F 2 populations and F 3 families developed from crosses between the original inbred and the NIL. The NC264 × SC76 F 2 population was tested in both long and short‐day photoperiod environments. The RFLP analysis of the F 2 individuals and F 3 families identified major QTLs for days to flowering and plant height on chro mosomes 1, 8, and 10. Major QTLs for total leaf number were found on chromosomes 1 and 8. Single‐factor analysis of variance techniques were employed for all pairwise marker‐trait associations. Additive gene action predominated at all loci. The most significant effects were constant across environments, generations, and populations except for the region on chromosome 8, which was not significant in the shortday photoperiod environment. Thus, the maturity QTL on chromosome 8 may represent a photoperiod response element. Selective determination of genotype using only the top and bottom 10% of the phenotypic extremes to identify QTLs was as effective as analysis of the entire population for detecting the most significant marker‐trait associations.
Opioid antagonist effects of dezocine in opioid-dependent humans* (1996)

Dezocine is an opioid mu-partial agonist recently approved for use as an analgesic in the United States. This study characterized the relative agonist versus antagonist effects of dezocine in comparison to naloxone (an opioid antagonist), hydromorphone (an opioid mu-agonist), and placebo (saline solution) in opioid-dependent volunteers. In a residential laboratory, six volunteer male opioid abusers maintained on 30 mg/day oral methadone underwent pharmacologic challenges two to three times per week, 20 hours after the last dose of methadone. Challenges consisted of a double-blind intramuscular injection of dezocine (dose range, 7.5 to 60 mg), hydromorphone (5 and 10 mg), naloxone (0.1 and 0.2 mg), or saline solution. Measures included physiologic indexes, self-reports of drug effects, and observer ratings of drug effects. Naloxone and hydromorphone produced characteristic antagonist-like and agonist-like effects, respectively. Dezocine acted as an opioid antagonist, precipitating a withdrawal syndrome only slightly different from that produced by naloxone. Dezocine's antagonist effects were not directly dose related, but peaked at intermediate doses and declined at higher doses.
Neutralizing Capacity of Monoclonal Antibodies That Recognize Peptide Sequences Underlying the Carbohydrates on gp41 of Simian Immunodeficiency Virus (2012)

Extensive glycosylation of the envelope spikes of human and simian immunodeficiency virus (HIV and SIV) is an important factor for the resistance of these viruses to neutralization by antibodies. SIVmac239 gp41 has three closely spaced sites for N-linked carbohydrate attachment. Rhesus macaques experimentally infected with mutant versions of SIVmac239 lacking two or three of these carbohydrate sites developed strong serum reactivity against mutated peptide sequences at the site of these glycosylations, as well as high titers of neutralizing activity to the mutant viruses (E. Yuste et al., J. Virol. 82:12472-12486, 2008). However, whether antibodies that recognize these underlying peptides have neutralizing activity has not been directly demonstrated. Here we describe the isolation and characterization of three gp41-specific monoclonal antibodies (4G8, 6G8, and 7D6) from one of these mutant-infected monkeys. All three antibodies reacted with mutant gp41 from viral particles and also with peptides corresponding to mutated sequences. Slight differences in peptide specificities were observed among the three antibodies. Sequence analysis revealed that the heavy chains of all three antibodies were derived from the same germ line heavy-chain segment (IGHV4-59*01), but they all had very different sequences in complementarity-determining region 3. The light chains of all three antibodies were very closely related to one another. All three antibodies had neutralizing activity to mutant viruses deficient in gp41 carbohydrate attachment, but they did not neutralize the parental SIVmac239. These results demonstrate unambiguously that antibodies with specificity for peptide sequences underlying gp41 carbohydrates can effectively neutralize SIV when these carbohydrates are absent. However, the presence of these gp41 carbohydrates effectively shields the virus from antibodies that would otherwise neutralize viral infectivity.
Electronic Structure of Deformed Carbon Nanotubes (2000)

Electronic structure of deformed carbon nanotubes varies widely depending on their chirality and deformation mode. We present a framework to analyze these variations by quantifying the dispersion relation and density of states. The theory is based on the Huckel tight-binding model and confirmed by four orbital tight-binding simulations of nanotubes under stretching, compression, torsion, and bending. It unriddles and unifies previous band gap studies and predicts the shifting, merging, and splitting of Van Hove singularities in the density of state, and the zigzag pattern of band gap change with strains. Possible applications to nanotube devices and spectroscopy research are also presented.
Terahertz photoconductive antenna with metal nanoislands (2012)

This work presents a nanoplasmonic photoconductive antenna (PCA) with metal nanoislands for enhancing terahertz (THz) pulse emission. The whole photoconductive area was fully covered with metal nanoislands by using thermal dewetting of thin metal film at relatively low temperature. The metal nanoislands serve as plasmonic nanoantennas to locally enhance the electric field of an ultrashort pulsed pump beam for higher photocarrier generation. The plasmon resonance of metal nanoislands was achieved at an excitation laser wavelength by changing the initial thickness of metal film. This nanoplasmonic PCA shows two times higher enhancement for THz pulse emission power than a conventional PCA. This work opens up a new opportunity for plasmon enhanced large-aperture THz photoconductive antennas.
Acute Renal Failure and Hypercalcemia (2006)

Hypercalcemia can result from excessive bone resorption, renal calcium retention, excessive intestinal calcium absorption, or a combination of these conditions. Hypercalcemia may also provoke acute renal failure (ARF) or hypertension, or aggravate the tubular necrosis that is frequently found in cases of ARF. The association of ARF and hypercalcemia was studied retrospectively in eight patients based in the data in their charts. Data are expressed as median and percentile (25th; 75th). Our results show that ARF associated with hypercalcemia was related with comorbidity in all cases (cancer, multiple myeloma, hyperparathyroidism, sarcoidosis, vitamin D intoxication, and leprosy). Maximum median serum creatinine levels were 3.3 mg/dL (2.7, 3.8 mg/dL) before treatment and 1.1 mg/dL (0.9, 1.3 mg/dL) after treatment. Maximum total median serum calcium was 15.9 mg/dL (13.5, 19.8 mg/dL) before treatment and 9.1 mg/dL (8.4, 9.7 mg/dL) after treatment. Maximum median ionized serum calcium was 2.1 mmol/L (1.8, 2.2 mmol/L) before treatment and 1.1 mmol/L (1.0, 1.2 mmol/L) after treatment. Different kinds of treatment induced a rapid fall in serum calcium concentration. All patients were treated with hydration and diuretics, and three patients also received calcitonin. Serum creatinine concentration always fell simultaneously with the decrease in serum calcium in all cases. All patients progressed with nonoliguric renal failure. In conclusion, in ARF, patients are frequently hypocalcemic. Usually, the presence of hypercalcemia associated with ARF is indicative of the presence of comorbidity, as observed in all eight patients studied here. There was an improvement of renal function in all cases as serum calcium levels decreased.
Molecular Determinants of Inverse Agonist Activity of Biologicals Targeting CTLA-4 (2007)

Ligation of CD28 or CTLA-4 with some biologicals can activate T cells due to an unexpected superagonist or inverse agonist activity, respectively. The risk of such an outcome limits the therapeutic development of these reagents. Thus, identifying the molecular determinants of superagonist/inverse agonist properties for biologicals targeting costimulatory/inhibitory receptors has not only fundamental value but also important therapeutic implications. In this study, we show that ligation of CTLA-4 with either soluble B7.1 Ig (but not B7.2 Ig) or with a recombinant bispecific in-tandem single chain Fv known as 24:26 induces TCR-independent, T cell activation. Such an inverse agonist activity requires CD28 expression and high CTLA-4 expression and is not seen when CTLA-4 is ligated by membrane-bound B7.1 or B7.2. At the molecular level, the inverse agonist activity of B7.1 Ig or 24:26 correlates with their ability to induce the formation of unique dimer-based, CTLA-4 oligomers on the T cell surface and involves CTLA-4 signaling through its cytoplasmic domain. Our results provide a potential mechanism to explain and to predict inverse agonist activity for CTLA-4 ligands.
Generation of Human Islets Through Expansion and Differentiation of Non-islet Pancreatic Cells Discarded (Pancreatic Discard) After Islet Isolation (2006)

Using the specified culture methods, non-islet pancreas cells can generate cell clusters resembling islets. These ICCs, obtained from fractions of the pancreas that are otherwise discarded, continue to differentiate after transplantation to become mature islets.
Identification of the Adult Human Hemangioblast (2004)

Recent studies show that human CD133(+) (previously known as AC133(+)) cells from mobilized peripheral blood consist of stem cells with either hematopoietic or endothelial potential. To test whether this population also contains individual precursors with both capacities, the defining characteristics of the elusive adult hemangioblast, we developed a culture system that allows single-cell analyses of differentiation. In the presence of vascular endothelial growth factor (VEGF), stem cell growth factor (SCGF), and FLT-3 ligand, CD133(+)-enriched cells were first expanded and the amplified cells were transduced with a vector encoding an enhanced green fluorescent protein (EGFP) marker gene. Single EGFP(+) cells were then cocultured with corresponding non-transduced cells from the same experiment, yielding 50-100 marked cells in 8% of the wells after 2 weeks. The resultant cells were divided and differentiated with either granulocyte colony-stimulating factor (G-CSF) or with SCGF and VEGF. These culture conditions resulted in the formation of neutrophil or endothelial cells, respectively, as identified morphologically and by phenotypic staining. Dual differentiation of EGFP(+) cells could be observed in one-quarter of clones from single-seeded cells, suggesting that 2% of EGFP(+) cells were in fact human hemangioblasts. These cells could be expanded for at least 28 days without losing this dual capacity. Hence, this culture system may be of clinical relevance in the development of cellular therapies for disorders involving hematopoiesis and the vascular system. In addition, our results provide important information related to the development of the vasculature and the potential role of hemangioblasts in vasculogenesis in adult humans.
Cellular immunity to viral antigens limits E1-deleted adenoviruses for gene therapy. (1994)

An important limitation that has emerged in the use of adenoviruses for gene therapy has been loss of recombinant gene expression that occurs concurrent with the development of pathology in the organ expressing the transgene. We have used liver-directed approaches to gene therapy in mice to study mechanisms that underlie the problems with transient expression and pathology that have characterized in vivo applications of first-generation recombinant adenoviruses (i.e., those deleted of E1a and E1b). Our data are consistent with the following hypothesis. Cells harboring the recombinant viral genome express the transgene as desired; however, low-level expression of viral genes also occurs. A virus-specific cellular immune response is stimulated that leads to destruction of the genetically modified hepatocytes, massive hepatitis, and repopulation of the liver with nontransgene-containing hepatocytes. These findings suggest approaches for improving recombinant adenoviruses that are based on further crippling the virus to limit expression of nondeleted viral genes.
Is There an Alternative Pathway for Starch Synthesis? (1992)

In leaf tissue, carbon enters starch via the gluconeogenesis pathway where d-glycerate 3-phosphate formed from CO(2) fixation is converted into hexose monophosphates within the chloroplast stroma. In starch-containing sink organs, evidence has been obtained indicating that the flow of carbon into starch follows a different pathway whereby hexose monophosphates formed from sucrose are transported into the amyloplast, a plastid specialized in starch accumulation. In both chloroplasts and amyloplasts, the formation of ADPglucose, the substrate for starch synthase, is controlled by the activity of ADPglucose pyrophosphorylase, a key regulatory enzyme of starch synthesis localized in the plastid. Recently, an alternative pathway of starch synthesis has been proposed in which ADPglucose is synthesized from sucrose and transported directly into the plastid compartment, where it is used for starch synthesis. On the basis of the biochemical phenotypes exhibited by various plant mutants with defined genetic lesions, it is concluded that ADPglucose pyrophosphorylase is essential for starch synthesis, whereas the alternative pathway has only a minor role in this process.
Chronic, controlled GDNF infusion promotes structural and functional recovery in advanced parkinsonian monkeys (2002)

The powerful trophic effects that glial cell line-derived neurotrophic factor (GDNF) exerts on midbrain dopamine neurones suggest its use in treating Parkinson's disease. However, some important questions remain about the possible therapeutic applications of GDNF. Here we demonstrate that the chronic infusion of 5 or 15 micro g/day GDNF into the lateral ventricle or the striatum, using programmable pumps, promotes restoration of the nigrostriatal dopaminergic system and significantly improves motor functions in rhesus monkeys with neural deficits modelling the terminal stages of Parkinson's disease. The functional improvements were associated with pronounced upregulation and regeneration of nigral dopamine neurones and their processes innervating the striatum. When compared with vehicle recipients, these functional improvements were associated with (i) >30% bilateral increase in nigral dopamine neurone cell size; (ii) >20% bilateral increase in the number of nigral cells expressing the dopamine marker tyrosine hydroxylase; (iii) >70 and >50% bilateral increase in dopamine metabolite levels in the striatum and the pallidum, respectively; (iv) 233 and 155% increase in dopamine levels in the periventricular striatal region and the globus pallidus, respectively, on the lesioned side; and (v) a five-fold increase in tyrosine hydroxylase-positive fibre density in the periventricular striatal region on the lesioned side. In addition, chronic GDNF treatment did not induce the side-effects generally associated with chronic administration of levodopa, the most widely used treatment for Parkinson's disease. Thus, the results suggest that the prolonged and controlled delivery of GDNF into the brain could be used to intervene in long-term neurodegenerative disease processes like Parkinson's disease. Additional studies are required to determine the potential differences between chronic, intraventricular and intraputamenal (or intranigral) delivery of GDNF to maximize the efficacy of infusion treatments.
Tandem MS can distinguish hyaluronic acid from N-acetylheparosan (2007)

Isobaric oligosaccharides enzymatically prepared from hyaluronic acid (HA) and N-acetylheparosan (NAH), were distinguished using tandem mass spectrometry. The only difference between the two series of oligosaccharides was the linkage pattern (in HA 1-->3 and in NAH 1-->4) between glucuronic acid and N-acetylglucosamine residues. Tandem mass spectrometry afforded spectra in which glycosidic cleavage fragment ions were observed for both HA and NAH oligosaccharides. Cross-ring cleavage ions 0,2An and 0,2An-h (n is even number) were observed only in GlcNAc residues of NAH oligosaccharides. One exception was an 0,2A2 ion fragment observed for the disaccharide from HA. These cross-ring cleavage fragment ions are useful to definitively distinguish HA and NAH oligosaccharides.
Cystic fibrosis-- current topics (1993)

Cell Cultures in CF Research Structure-Function Relations of CFTR Regulation of CFTR Channel Activity Evolution of CF Alleles Psychological Aspect of CF Heterozygote Screening Congenital Absence of Vas Deferens Aspergillus Infection in CF Dnase and Other Mycolytics Clinical Management of CF Patients Awaiting and Immediately Following Lung Transplantation CF Clinical Scoring Systems.