gebhart/patrepeval huggingface dataset:
Surface Gradient Material:  From Superhydrophobicity to Superhydrophilicity (2006)

We have developed a simple method to fabricate a gradient from superhydrophobicity to superhydrophilicity. It is based on the controlled self-assembled monolayer consisting of a thiol molecule on a gold surface and the amplifying effect of the wetting property on a rough surface. Using a relatively dilute HS(CH2)11CH3 solution (0.05 mmol/L), we found that the density of molecules on the surface can be controlled by varying the immersion time. Slowly adding the dilute solution to the container holding the rough gold substrate will lead to a density gradient along the surface. After the complementary adsorption of HS(CH2)10CH2OH, the surface exhibits a gradient from superhydrophobicity to superhydrophilicity. The slope of the gradient can be conveniently tuned by varying the speed of addition. Cassie-Baxter and Wenzel equations are employed to explain this special property based on the rough structure and the molecular composition gradient that have been determined by XPS. This kind of material would provide a larger oriented driving force for many important biological and physical processes and might have potential applications in water droplet movement, oriented axonal specification of neurons, protein adhesion, and so on.
Two-dimensional block diagonal LMS adaptive filtering (1994)

The paper is concerned with the development of two-dimensional (2D) adaptive filters using the block diagonal least mean squared (BDLMS) method. In this adaptive filtering scheme, the image is scanned and processed block by block in a diagonal fashion, and the filter weights are adjusted once per block rather than once per pixel. The diagonal scanning is adopted to avoid the problems inherent in the 1D standard scanning schemes and to account for the correlations in two directions. The weight updating equation for 2D BDLMS is derived, and the convergence properties of the algorithms are investigated. Simulation results that indicate the effectiveness of the 2D BDLMS when used for image enhancement, estimation, and detection applications are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Familial Dilated Cardiomyopathy Locus Maps to Chromosome 2q31 (1999)

A dilated cardiomyopathy locus (designated CMD1G) is located on chromosome 2q31 and causes early-onset congestive heart failure. Although titin remains an intriguing candidate gene for this disorder, a disease-causing mutation is not present in its cardiac-specific N2-B domain.
Effect of Intra-articular Injection of Hyaluronic Acid on the Clinical Symptoms of Osteoarthritis and on Granulation Tissue Formation (1971)

1From Ihc Department of Connective Tissue Research. Boston Biomcdical Research Institute, Boston, Massachusetts 2From the Orthopedic Clinic, Harvard Medical School. Massachusetts General Hospital, Boston, Massachusetts.
Unbiased Charged Circular CMUT Microphone: Lumped-Element Modeling and Performance (2017)

An energy-consistent lumped-element equivalent circuit model for charged circular capacitive micromachined ultrasonic transducer (CMUT) cell is derived and presented. It is analytically shown and experimentally verified that a series dc voltage source at the electrical terminals is sufficient to model the charging in CMUT. A model-based method for determining this potential from impedance measurements at low bias voltages is presented. The model is validated experimentally using an airborne CMUT, which resonates at 103 kHz. Impedance measurements, reception measurements at resonance and off-resonance, and the transient response of the CMUT are compared with the model predictions.
Highly Oxidation-Resistant TiN Barrier Layers for Ferroelectric Capacitors (1997)

The characteristics of TiN film, which is well suited for use as the barrier layer of ferroelectric capacitors, were investigated. It was found that high-density TiN is more resistive against oxidation than low-density one. It was also revealed that the &lt;200&gt;-oriented TiN had higher oxidation resistance than the &lt;111&gt;-oriented one. However, whereas a thick Pt bottom electrode was previously used to prevent barrier layer oxidation, we found that the Pt thickness can be reduced to less than half with high-density &lt;200&gt;-oriented TiN. TiN oxidation followed the linear-oxidation law (a reaction-limited process) in the initial stage of oxidation, and the oxidation rate was found to be inversely proportional to the density and proportional to the etching rate. The etching rate of the &lt;200&gt;-oriented TiN was lower than that of the &lt;111&gt;-oriented TiN, indicating that (100) was less reactive than (111). Consequently, the &lt;200&gt;-oriented TiN film exhibited high oxidation resistance.
Expression of CD39 on Activated T Cells Impairs their Survival in Older Individuals (2016)

In an immune response, CD4(+) T cells expand into effector T cells and then contract to survive as long-lived memory cells. To identify age-associated defects in memory cell formation, we profiled activated CD4(+) T cells and found an increased induction of the ATPase CD39 with age. CD39(+) CD4(+) T cells resembled effector T cells with signs of metabolic stress and high susceptibility to undergo apoptosis. Pharmacological inhibition of ATPase activity dampened effector cell differentiation and improved survival, suggesting that CD39 activity influences T cell fate. Individuals carrying a low-expressing CD39 variant responded better to vaccination with an increase in vaccine-specific memory T cells. Increased inducibility of CD39 after activation may contribute to the impaired vaccine response with age.
Cyclin-Binding Motifs Are Essential for the Function of p21CIP1 (1996)

The cyclin-dependent kinase (Cdk) inhibitor p21 is induced by the tumor suppressor p53 and is required for the G1-S block in cells with DNA damage. We report that there are two copies of a cyclin-binding motif in p21, Cy1 and Cy2, which interact with the cyclins independently of Cdk2. The cyclin-binding motifs of p21 are required for optimum inhibition of cyclin-Cdk kinases in vitro and for growth suppression in vivo. Peptides containing only the Cy1 or Cy2 motif partially inhibit cyclin-Cdk kinase activity in vitro and DNA replication in Xenopus egg extracts. A monoclonal antibody which recognizes the Cy1 site of p21 specifically disrupts the association of p21 with cyclin E-Cdk2 and with cyclin D1-Cdk4 in cell extracts. Taken together, these observations suggest that the cyclin-binding motif of p21 is important for kinase inhibition and for formation of p21-cyclin-Cdk complexes in the cell. Finally, we show that the cyclin-Cdk complex is partially active if associated with only the cyclin-binding motif of p21, providing an explanation for how p21 is found associated with active cyclin-Cdk complexes in vivo. The Cy sequences may be general motifs used by Cdk inhibitors or substrates to interact with the cyclin in a cyclin-Cdk complex.
Epitope mapping of the Pseudomonas aeruginosa major outer membrane porin protein OprF (1995)

The Pseudomonas aeruginosa major outer membrane protein OprF has been proposed for use as a vaccine and as a target for immunotherapeutic and diagnostic monoclonal antibodies. The well-conserved epitopes for 10 surface-reactive, OprF-specific monoclonal antibodies were localized by both overlapping peptide analysis and immunodetection of OprF peptides generated by cyanogen bromide and the protease papain. Three of the monoclonal antibodies bound to specific overlapping octapeptides, which had been synthesized on 160 pins to cover the entire 326 amino acids of OprF. The highest reactivities were as follows: MA7-1 to the pin with attached peptide GTYETGNK (amino acids 55 to 62), MA7-2 to NLADFMKQ (amino acids 237 to 244), and MA5-8 to TAEGRAIN (amino acids 307 to 314). The other monoclonal antibodies showed no reactivity, indicating that they do not recognize linear epitopes. Two polyclonal sera were also tested and demonstrated weak reactivity with discrete regions of OprF, suggesting that the majority of antibodies produced might recognize conformational epitopes. Utilizing defined peptides generated with cyanogen bromide and papain, the conformational epitopes recognized by the seven monoclonal antibodies were localized to regions that were 42 to 90 amino acids long. These regions were located on two adjacent loops in the middle of an amended structural model of OprF.
Smart tags: the distributed-memory revolution (1989)

Electronic identification and data-storage badges can now be used to keep track of people, animals or car bodies, without need for central computer or physical contact
Increased defecation during stress or after 5‐hydroxytryptophan: selective inhibition by the 5‐HT4 receptor antagonist, SB‐207266 (2000)

5-HT(4) receptor antagonism prevents the ability of exogenous 5-HT or 5-HTP to sensitize the intestinal peristaltic reflex and increase the rate of defecation, generally without affecting non-stimulated intestinal function. In this study we confirmed the ability of the selective 5-HT(4) receptor antagonist SB-207266 1 - 1000 microg kg(-1) p.o., to prevent the increase in defecation evoked over a 60 min period by 5-HTP 10 mg kg(-1) s.c. in conscious mice, in the absence of an apparent constipating action. The role of endogenous 5-HT in the mechanisms of increased defecation and/or diarrhoea was then investigated in conscious, fed rats. This was evoked by 180 min exposure to restraint stress, which increased both the number and mean weight of formed, faecal pellets excreted over the entire time period. SB-207266 1 - 1000 microg kg(-1) p.o. (dosed 30 min before restraint) did not affect the increase in defecation evoked during the first 60 min of restraint stress, but significantly and dose-dependently reduced or prevented the increased defecation during the remaining 120 min of the experiment; this action occurred in the absence of an apparent constipating action of SB-207266. In fasted rats exposed to restraint stress, watery diarrhoea developed and although there was a tendency for SB-207266 1 - 1000 microg kg(-1) p.o. (dosed 30 min before restraint) to reduce the incidence of diarrhoea, this inhibition was not complete. We conclude that selective 5-HT(4) receptor antagonism prevents disruptions in defecation behaviours caused by exogenous or endogenous enteric 5-HT and that this activity is not accompanied by a concomitant suppression of activity (constipation-like) within the intestine itself.
Deep sleep (2015)

Boreholes drilled into Earth's crust get a fresh look for nuclear waste disposal.
Isolation and characterization of human mammary stem cells (2005)

Since stem cells are present throughout the lifetime of an organism, it is thought that they may accumulate mutations, eventually leading to cancer. In the breast, tumours are predominantly oestrogen and progesterone receptor-positive (ERalpha/PR+). We therefore studied the biology of ERalpha/PR-positive cells and their relationship to stem cells in normal human mammary epithelium. We demonstrated that ERalpha/PR-positive cells co-express the putative stem cell markers p21(CIP1/WAF1), cytokeratin (CK) 19 and Musashi-1 when examined using dual label immunofluorescence on tissue sections. Next, we isolated a Hoechst dye-effluxing 'side population' (SP) from the epithelium using flow cytometry and demonstrated them to be undifferentiated cells by lack of expression of myoepithelial and luminal cell-specific antigens such as CALLA and MUC1. Epithelial SP cells were shown to be enriched for the putative stem cell markers p21(CIP1/WAF1), Musashi-1 and ERalpha/PR-positive cells. Lastly, SP cells, compared to non-SP, were highly enriched for the capacity to produce colonies containing multiple lineages in 3D basement membrane (Matrigel) culture. We conclude that breast stem cells include two populations: a primitive ERalpha/PR-negative stem cell necessary for development and a shorter term ERalpha/PR-positive stem cell necessary for adult tissue homeostasis during menstrual cycling. We speculate these two basic stem cell types may therefore be the cells of origin for ERalpha-positive and -negative breast tumours.
Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom. Further evidence for an exendin receptor on dispersed acini from guinea pig pancreas. (1992)

The recent identification in Heloderma horridum venom of exendin-3, a new member of the glucagon superfamily that acts as a pancreatic secretagogue, prompted a search for a similar peptide in Heloderma suspectum venom. An amino acid sequencing assay for peptides containing an amino-terminal histidine residue (His1) was used to isolate a 39-amino acid peptide, exendin-4, from H. suspectum venom. Exendin-4 differs from exendin-3 by two amino acid substitutions, Gly2-Glu3 in place of Ser2-Asp3, but is otherwise identical. The structural differences make exendin-4 distinct from exendin-3 in its bioactivity. In dispersed acini from guinea pig pancreas, natural and synthetic exendin-4 stimulate a monophasic increase in cAMP beginning at 100 pM that plateaus at 10 nM. The exendin-4-induced increase in cAMP is inhibited progressively by increasing concentrations of the exendin receptor antagonist, exendin-(9-39) amide. Unlike exendin-3, exendin-4 does not stimulate a second rise in acinar cAMP at concentrations greater than 100 nM, does not stimulate amylase release, and does not inhibit the binding of radiolabeled vasoactive intestinal peptide to acini. This indicates that in dispersed pancreatic acini, exendin-4 interacts only with the recently described exendin receptor.
Does Inflammation Cut to the Heart of the Matter? (2002)

CARDIOVASCULAR DISEASEA growing body of evidence suggests that a molecular marker for inflammation known as C-reactive protein (CRP) may be as crucial as cholesterol deposition and clogged arteries in assessing risk of heart attack. That discovery, the result of considerable work over the past decade, has spawned more questions, starting with the most fundamental one: Does the presence of inflammation trigger rising CRP levels that lead to heart disease, or is inflammation a byproduct of the disease? The answer could greatly affect the standard of care for the disease, from early diagnosis through treatment.
Effect on Electrical Properties of Barium and Strontium Additions in the Lead Lanthanum Zirconate Stannate Titanate System (1997)

The effect of barium (Ba) and strontium (Sr) additions on the electrical properties of lead lanthanum zirconate stannate titanate (PLZST) ceramics and their electrically induced phase change behavior was examined. Sr additions decreased both the ferroelectric (FE) ↔ antiferroelectric (AFE) transition temperature ( T AFE‐FE ) and the temperature of the maximum dielectric constant ( T MAX ). This indicates that Sr additions stabilize the AFE phase. Along with B‐site modifications Sr additions can be used to decrease the switching field and hysteresis. Ba additions caused the temperature range of the AFE phase to narrow by increasing T AFE‐FE and decreasing T MAX . This behavior indicates an ability to lower the switching field and suppress hysteresis. Using a combination of both A‐site and B‐site modifications it is possible to tailor the properties (for example, switching field, hysteresis, and operating temperature range) of this group of phase change materials to meet the requirements of specific applications.
Enhanced Detection of Focal Brain Responses Using Intersubject Averaging and Change-Distribution Analysis of Subtracted PET Images (1988)

Intersubject averaging and change-distribution analysis of subtracted positron emission tomographic (PET) images were developed and tested. The purpose of these techniques is to increase the sensitivity and objectivity of functional mapping of the human brain with PET. To permit image averaging, all primary tomographic images were converted to anatomically standardized three-dimensional images using stereotactic anatomical localization and interslice interpolation. Image noise, measured in control-minus-control subtractions, was strongly suppressed by averaging. Signal-to-noise ratio, measured in stimulus-minus-control subtractions (hand vibration minus eyes-closed rest), rose steadily with averaging, confirming the accuracy of our method of anatomical standardization. Distribution analysis of CBF change images (outlier detection by gamma-2 statistic) was assessed as an omnibus test for state-dependent changes in regional neuronal activity. Sensitivity in detecting the somatosensory response rose steadily with averaging, increasing from 50% in individual images to 100% when three or more images were averaged. Specificity was 100% at all averaging levels. Although described here as a technique for functional brain mapping with H2(15O) CBF images, image averaging, and change-distribution analysis are more generally applicable techniques, not limited to a single purpose or tracer.
p16 proteins from melanoma-prone families are deficient in binding to Cdk4. (1995)

The tumor suppressor candidate p16INK4 is a cyclin-dependent kinase inhibitor that inhibits cell proliferation. The p16 coding gene is often mutated in glioblastomas, pancreatic adenocarcinomas and melanoma-prone pedigrees, but, until recently, the significance of these allelic variants has remained unclear. Here, we used interaction mating and coprecipitation to measure interaction of seven p16 allelic variants detected in melanoma-prone pedigrees with Cyclin-dependent kinases (Cdks). We found that most variants were deficient in interaction with Cdk4 and Cdk6. One defective variant was found both in cancer prone families and in the control population and therefore previously defined as a common polymorphism. Another variant, which is weakly linked to familial cancer, is only slightly affected in interaction with Cdks. These results are consistent with the idea that p16 allelic variants that decrease Cdk interaction predispose individuals who carry them to an increased risk of cancer. Moreover, they suggest that determination of affinity between p16 mutants and partner proteins may help identify functionally-significant allelic variants not detected by classical human genetic techniques.
Human Metapneumovirus Infection in the Canadian Population (2003)

Human metapneumovirus (hMPV), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (ARIs) ranging from upper ARIs to severe bronchiolitis and pneumonia. Important questions remain on the contribution of hMPV to ARIs and its impact on public health. During the 2001-2002 season, we conducted a collaborative study with four provincial public health laboratories to study the prevalence of this new virus in the Canadian population. A total of 445 specimens were collected from patients of all age groups with ARIs and were tested for the presence of hMPV by reverse transcription-PCR. Of these, 66 (14.8%) tested positive for hMPV. Positive specimens were found in all age groups and in all four provinces studied. Virus activity peaked in February and March. The age range of the patients with hMPV infection was 2 months to 93 years (median age, 25 years), with similar numbers of females (35%) and males (41%). Thirty-three percent (n = 22) of hMPV-infected patients were hospitalized; of these, 27% (n = 6) had rhinitis and pneumonia, 23% (n = 5) had bronchiolitis, and 9% (n = 2) had bronchitis. The hospitalization rates were significantly higher among patients <5 years of age (P = 0.0005) and those >50 years of age (P = 0.0044) than among those 6 to 50 years of age. Phylogenetic analysis of the F gene showed that two hMPV genetic clusters were cocirculating in the 2001-2002 season, and comparison with earlier studies suggests a temporal evolutionary pattern of hMPV isolates. These results provide further evidence of the importance of hMPV in ARIs, particularly in young children and elderly individuals.
Impact of ion-host interactions on the5d-to-4fspectra of lanthanide rare-earth-metal ions. II. The Ce-doped elpasolites (1986)

Crystal growth of Ce-doped fluoride elpasolites is reported. Single crystals of ${\mathrm{Rb}}_{2}$${\mathrm{NaYF}}_{6}$ and ${\mathrm{Rb}}_{2}$${\mathrm{NaScF}}_{6}$ have been obtained by both vertical Bridgman and Czochralski techniques. Attempts to grow ${\mathrm{K}}_{2}$${\mathrm{NaYF}}_{6}$ by both methods yielded only polycrystalline samples. Ce-doped ${\mathrm{Rb}}_{2}$${\mathrm{NaYF}}_{6}$ and ${\mathrm{K}}_{2}$${\mathrm{NaYF}}_{6}$ display two distinct emitting centers, one in the blue and another in the near uv. Ce:${\mathrm{Rb}}_{2}$${\mathrm{NaScF}}_{6}$ displays only the blue-emitting center, and Ce:${\mathrm{K}}_{2}$${\mathrm{NaScF}}_{6}$ displays only the uv-emitting center. Growth and spectroscopic investigation of ${\mathrm{Rb}}_{2}$${\mathrm{NaYF}}_{6}$ doped with other rare earths established that the blue-emitting center is ${\mathrm{Ce}}^{3+}$ in the normal (Y or Sc) site. The uv-emitting center is ${\mathrm{Ce}}^{3+}$ in a larger, lower-symmetry site. It is hypothesized that this second site is related either to disordering or to the intergrowth of a second phase. Absorption, emission, excitation, and vacuum-uv transmission spectra were measured on Ce-doped elpasolites. Covalent contributions to the crystal field are numerically estimated by comparing the observed 5d energy levels with those predicted by an ionic-model calculation. These estimates as well as the qualitative dependence of the 5d energies on the lattice constant indicate that covalent contributions to the crystal field are significant. Design rules are suggested for choosing a fluoride host crystal for a visible-wavelength ${\mathrm{Ce}}^{3+}$ laser.
Lineage Conversion of Murine Extraembryonic Trophoblast Stem Cells to Pluripotent Stem Cells (2011)

In mammals, the first cell fate decision is initialized by cell polarization at the 8- to 16-cell stage of the preimplantation embryo. At this stage, outside cells adopt a trophectoderm (TE) fate, whereas the inside cell population gives rise to the inner cell mass (ICM). Prior to implantation, transcriptional interaction networks and epigenetic modifications divide the extraembryonic and embryonic fate irrevocably. Here, we report that extraembryonic trophoblast stem cell (TSC) lines are converted to induced pluripotent stem cells (TSC-iPSCs) by overexpressing Oct4, Sox2, Klf4, and cMyc. Methylation studies and gene array analyses indicated that TSC-iPSCs had adopted a pluripotent potential. The rate of conversion was lower than those of somatic reprogramming experiments, probably due to the unique genetic network controlling extraembryonic lineage fixation. Both in vitro and in vivo, TSC-iPSCs differentiated into tissues representing all three embryonic germ layers, indicating that somatic cell fate could be induced. Finally, TSC-iPSCs chimerized the embryo proper and contributed to the germ line of mice, indicating that these cells had acquired full somatic differentiation potential. These results lead to a better understanding of the molecular processes that govern the first lineage decision in mammals.