gebhart/patrepeval huggingface dataset:
Role of the non‐respiratory pathways in the utilization of molecular oxygen by Saccharomyces cerevisiae (2003)

Saccharomyces cerevisiae is a facultative anaerobe devoid of mitochondrial alternative oxidase. In this yeast, the structure and biogenesis of the respiratory chain, on the one hand, and the functional interactions of oxidative phosphorylation with the cellular energetic metabolism, on the other, are well documented. However, to our knowledge, the molecular aspects and the physiological roles of the non-respiratory pathways that utilize molecular oxygen have not yet been reviewed. In this paper, we review the various non-respiratory pathways in a global context of utilization of molecular oxygen in S. cerevisiae. The roles of these pathways are examined as a function of environmental conditions, using either physiological, biochemical or molecular data. Special attention is paid to the characterization of the so-called 'cyanide-resistant respiration' that is induced by respiratory deficiency, catabolic repression and oxygen limitation during growth. Finally, several aspects of oxygen sensing are discussed.
A Homogenous Microarray for Enzymatic Functional Assays (2006)

Microarrays as an emerging research tool promises to play a pivotal role in the post genomic era. However, in spite of the fast development of this technology special requirements, such as the immobilization and delivery of bio-reagents on the chip surface limit the utilization of microarrays, especially for small chemical compound libraries. We have developed a unique homogenous microarray system that overcomes these limitations and can be used to array most biofunctional molecules, such as small chemical compounds, peptides and proteins without pre-immobilization. A standard microscope slide containing up to 5000 microarray dots, with volumes less than 2 nanoliter each and acting as individual reaction centers, can be printed with standard DNA arrayer. An aerosol deposition technology was adapted to deliver extremely small volumes of biofluids uniformly into each reaction center. The fluorescence based reaction signals were then scanned and analyzed with standard DNA scanner and DNA array analyzing software. With this platform, we demonstrated that this chip format could be used for not only screening individual but also multiple enzymatic activities simultaneously with different fluorescent tagged small peptide libraries. We further demonstrated that this system could be a very powerful ultra high throughput screening tool for drug discovery, with which we identified potential “hits” after screening chips printed with small chemical compounds against caspases 1 and 3. This highly sensible chip is also able to monitor caspase protein expression profiles by activating the peptide chips with cell lysates undergoing apoptosis.
Substituted Tris(2-pyridylmethyl)amine Ligands for Highly Active ATRP Catalysts (2012)

The synthesis and application of a very active catalyst for copper-catalyzed atom transfer radical polymerizations (ATRP) with tris([(4-methoxy-2,5-dimethyl)-2-pyridyl] methyl)amine (TPMA*) ligand is reported. Catalysts with TPMA* ligands are approximately 3 orders of magnitude more active than those with tris(2-pyridylmethyl)amine (TPMA). Catalyst activity was evaluated by cyclic voltammetry, stopped-flow, and ATRP kinetics. Catalysts with TPMA* ligands perform better than those with TPMA ligands, especially at low catalyst concentrations.
Tau oligomers in cerebrospinal fluid in Alzheimer's disease (2017)

These assays are suitable for the analysis of human CSF samples. These results here suggest that CSF tau oligomer measurements could be optimized and added to the panel of CSF biomarkers for the accurate and early detection of AD.
Distinguishing users with capacitive touch communication (2012)

As we are surrounded by an ever-larger variety of post-PC devices, the traditional methods for identifying and authenticating users have become cumbersome and time-consuming. In this paper, we present a capacitive communication method through which a device can recognize who is interacting with it. This method exploits the capacitive touchscreens, which are now used in laptops, phones, and tablets, as a signal receiver. The signal that identifies the user can be generated by a small transmitter embedded into a ring, watch, or other artifact carried on the human body. We explore two example system designs with a low-power continuous transmitter that communicates through the skin and a signet ring that needs to be touched to the screen. Experiments with our prototype transmitter and tablet receiver show that capacitive communication through a touchscreen is possible, even without hardware or firmware modifications on a receiver. This latter approach imposes severe limits on the data rate, but the rate is sufficient for differentiating users in multiplayer tablet games or parental control applications. Controlled experiments with a signal generator also indicate that future designs may be able to achieve datarates that are useful for providing less obtrusive authentication with similar assurance as PIN codes or swipe patterns commonly used on smartphones today.
Glass: Mechanics and Technology (2007)

INTRODUCTION GLASS, A CERAMIC MATERIAL Four Classes of Materials Ashby's Charts Co-Selecting Materials Performance Indexes Shape Factors in Mechanical Design GLASS PRE- AND HISTORY Natural Glasses Early Glasses First Optical Glasses Modern Glasses APPLICATIONS OF GLASSES Glazing Packaging Optical Glass Glass Fibres for Insulation and Reinforcement Abrasive Tools Glass Manufacturers GLASS STRUCTURE Introduction Silica Glass and Related Glasses Organic and Chalcogenide Glasses Avoiding Crystallization Surface Structure GLASS RHEOLOGY Viscosity Glass Transition and its Observation How to 'Observe' Glass Transition? Viscous Response of Glass Visco-Elastic Response of Glass Tempering of Glass MECHANICAL STRENGHT OF GLASS Theoretical Strength Tensile Resistance of Glass Stress Concentration Linear Elasticity Crack Tip Stress Field Toughness Measurement Influence of Residual Stress on Strength and Fragmentation Statistic Weibull Analysis CONTACT RESISTANVCE OF GLASS Sharp and Blunt Contact Sharp Contact Resistance Abrasion Resistance Cutting of Glass Application to other Materials AGEING OF GLASS Stress Corrosion Charles and Hillig Theory Life Time Under Static Fatigue Applications MECHANICS OF GLASS PROCESSES Introduction Float Process Blow-and-Blow and Press-and-Blow Process Fusion Draw PRODUCTION AND CONTROL OF RESIDUAL STRESSES Introduction Residual Stresses in Flat Glass Basics of Photoelasticity in Flat Glass Stress Meters HIGH-TECH PRODUCERS AND R&D Market Trend Driven R&D Flat Display Panel Thin Film Technology Residual Stresses in Thin Film Applications Conclusion APPENDICES
Septal networks: relevance to theta rhythm, epilepsy and Alzheimer's disease (2006)

Information processing and storing by brain networks requires a highly coordinated operation of multiple neuronal groups. The function of septal neurons is to modulate the activity of archicortical (e.g. hippocampal) and neocortical circuits. This modulation is necessary for the development and normal occurrence of rhythmical cortical activities that control the processing of sensory information and memory functions. Damage or degeneration of septal neurons results in abnormal information processing in cortical circuits and consequent brain dysfunction. Septal neurons not only provide the optimal levels of excitatory background to cortical structures, but they may also inhibit the occurrence of abnormal excitability states.
Review: IFN- α / β Receptor Interactions to Biologic Outcomes: Understanding the Circuitry (2002)

Type I interferons (IFNs), which include the IFN-alphas, IFN-beta, IFN-omega, IFN-kappa, and IFN-tau, are an evolutionarily conserved group of secreted cytokines that serve as potent extracellular mediators of host defense and homeostasis. Binding of IFNs to specific cell surface receptors results in the activation of multiple intracellular signaling cascades, leadingto the synthesis of proteins that mediate antiviral, growth inhibitory and immunomodulatory responses. In the past decade, considerable information has accumulated pertaining to the different signalingpathways that are activated by the type I IFNs. Although many of the literature findings are specific to defined cell systems or are tissue restricted, the intent of this review is to place these signaling cascades and their effectors in the context of distinct biologic outcomes.
Optimizing the expression of a monoclonal antibody fragment under the transcriptional control of the Escherichia coli lac promoter (2000)

The expression of a monoclonal antibody Fab fragment in Escherichia coli strain RB791/pComb3, induced with either lactose or isopropyl-beta-D-thiogalactoside (IPTG), was compared to determine if lactose might provide an inexpensive alternative to induction with IPTG. Induction of Fab expression imposed a metabolic load on the recombinant cells, resulting in lower final cell yields compared to the non-induced controls. An IPTG concentration of 0.05 mM was sufficient to achieve maximal expression of soluble Fab protein when inducing in the early-, mid-, or late-log phases of batch cultures grown using either glucose or glycerol as a carbon source. The largest overall yield of Fab fragments when using 0.05 mM IPTG was achieved by increasing the final yield of cells through glycerol feeding following induction in late-log phase. Lactose was as effective as IPTG for inducing Fab expression in E. coli RB791/pComb3. The greatest overall level of Fab expression was found when cells grown on glycerol were induced with 2 g/L lactose in late-log phase. Since the cost of 0.05 mM of IPTG is significantly greater than the cost of 2 g/L lactose, lactose provides an inexpensive alternative to IPTG for inducing the expression of Fab fragments, and possibly other recombinant proteins, from the E. coli lac promoter.
Three‐dimensional pancreas organogenesis models (2016)

A rediscovery of three-dimensional culture has led to the development of organ biogenesis, homeostasis and disease models applicable to human tissues. The so-called organoids that have recently flourished serve as valuable models bridging between cell lines or primary cells grown on the bottom of culture plates and experiments performed in vivo. Though not recapitulating all aspects of organ physiology, the miniature organs generated in a dish are useful models emerging for the pancreas, starting from embryonic progenitors, adult cells, tumour cells and stem cells. This review focusses on the currently available systems and their relevance to the study of the pancreas, of β-cells and of several pancreatic diseases including diabetes. We discuss the expected future developments for studying human pancreas development and function, for developing diabetes models and for producing therapeutic cells.
Detection of intermediary Clr with complete active site, using a synthetic proteinase inhibitor (1984)

The synthetic proteinase inhibitor, FUT-175 (6-amidino-2-naphthyl-4-guanidinobenzoate), strongly suppressed activation of Clr at 37 degrees C, causing 50% inhibition at 0.03 mM. To clarify whether the inhibitor was incorporated into the active site of intermediary Clr formed during the incubation, determination of the active site was tried using this inhibitor. Consequently, release of amidinonaphthol equimolar with the amount of Clr used was observed in the early period of incubation, in which the activation to Clr- was about 5%. These results indicate that intermediary Clr already has a complete active site.
Colony-Stimulating Factor-1 Suppresses Responses to CpG DNA and Expression of Toll-Like Receptor 9 but Enhances Responses to Lipopolysaccharide in Murine Macrophages (2002)

During bacterial infections, the balance between resolution of infection and development of sepsis is dependent upon the macrophage response to bacterial products. We show that priming of murine bone marrow-derived macrophages (BMMs) with CSF-1 differentially regulates the response to two such stimuli, LPS and immunostimulatory (CpG) DNA. CSF-1 pretreatment enhanced IL-6, IL-12, and TNF-alpha production in response to LPS but suppressed the same response to CpG DNA. CSF-1 also regulated cytokine gene expression in response to CpG DNA and LPS; CpG DNA-induced IL-12 p40, IL-12 p35, and TNF-alpha mRNAs were all suppressed by CSF-1 pretreatment. CSF-1 pretreatment enhanced LPS-induced IL-12 p40 mRNA but not TNF-alpha and IL-12 p35 mRNAs, suggesting that part of the priming effect is posttranscriptional. CSF-1 pretreatment also suppressed CpG DNA-induced nuclear translocation of NF-kappaB and phosphorylation of the mitogen-activated protein kinases p38 and extracellular signal-related kinases-1/2 in BMMs, indicating that early events in CpG DNA signaling were regulated by CSF-1. Expression of Toll-like receptor (TLR)9, which is necessary for responses to CpG DNA, was markedly suppressed by CSF-1 in both BMMs and thioglycolate-elicited peritoneal macrophages. CSF-1 also down-regulated expression of TLR1, TLR2, and TLR6, but not the LPS receptor, TLR4, or TLR5. Hence, CSF-1 may regulate host responses to pathogens through modulation of TLR expression. Furthermore, these results suggest that CSF-1 and CSF-1R antagonists may enhance the efficacy of CpG DNA in vivo.
Enhanced Bonding of Alkanephosphonic Acids to Oxidized Titanium Using Surface-Bound Alkoxyzirconium Complex Interfaces (1999)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTEnhanced Bonding of Alkanephosphonic Acids to Oxidized Titanium Using Surface-Bound Alkoxyzirconium Complex InterfacesEllen S. Gawalt, Gang Lu, Steven L. Bernasek, and Jeffrey SchwartzView Author Information Department of Chemistry, Princeton University, Princeton, New Jersey 08544-1009 Cite this: Langmuir 1999, 15, 26, 8929–8933Publication Date (Web):October 7, 1999Publication History Received12 July 1999Published online7 October 1999Published inissue 1 December 1999https://pubs.acs.org/doi/10.1021/la990906mhttps://doi.org/10.1021/la990906mbrief-reportACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views1005Altmetric-Citations93LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Deposition,Organophosphorus compounds,Oxides,Pretreatment,Thin films Get e-Alerts
New strategies in insulin treatment: analogues and noninvasive routes of administration (2004)

Abstract Recent years have seen the development of alternatives to human insulin for the treatment of diabetes. Both rapid-acting and long-acting analogues are available. Alternative routes of insulin administration are emerging. The present review briefly summarizes the present knowledge on insulin analogues and alternative administration routes.
Conceptually New 20-epi-22-Oxa Sulfone Analogues of the Hormone 1α,25-Dihydroxyvitamin D3:  Synthesis and Biological Evaluation (2000)

New C,D-ring side-chain-modified sulfone 4a, with natural 1alpha, 3beta-hydroxyl groups but lacking the 25-hydroxyl group characteristic of the natural hormone 1alpha,25-dihydroxyvitamin D(3) (1), has been prepared and characterized. Novel synthetic features include: (1) chemoselective oxidation of only a primary silyl ether in a primary-secondary bis-silyl ether intermediate and (2) smooth reductive etherification without interference by a neighboring sulfonyl group. Sulfone 4a, but not its 1beta, 3alpha-diastereomer 4b, is powerfully antiproliferative and transcriptionally active in vitro but desirably noncalcemic in vivo. Although sulfone 4a, designed to resemble Leo Pharmaceutical Co.'s KH-1060 (3), is recognized by catabolic enzymes, the selective biological profile of sulfone 4a is likely not due to its metabolites that are formed in only minor amounts.
The Complete cDNA Sequence and Structural Polymorphism of the Polypeptide Chain of Porcine Submaxillary Mucin (1997)

The complete structure of the DNA encoding the polypeptide chain of porcine submaxillary mucin has been determined. The polypeptide is composed of distinct domains. A large central domain containing tandem repeats of 81 residues each is flanked by much shorter domains with sequences similar to the tandem repeats. Four disulfide-rich domains, three at the amino terminus and one at the carboxyl terminus, complete the chain. The disulfide-rich domains have significant sequence identity to those of other mucins and prepro-von Willebrand factor. The coding region of the mucin gene is highly polymorphic, and three alleles were identified in a single animal that encoded different numbers of the 81-residue tandem repeats. A single large exon devoid of introns encodes the tandem repeat domains. The largest allele with 135 tandem repeats encoded 13,288 amino acids to give a polypeptide with Mr = 1,184,106. The other two alleles contained 99 and 125 tandem repeats, respectively. Each allele also showed different restriction fragment length polymorphisms, which is consistent with the different patterns seen in individual animals. Fragment length polymorphism was also seen within two different families of animals, indicating that the polymorphism observed occurs in a single generation.
After Hard Drives—What Comes Next? (2009)

There are numerous emerging nonvolatile memory technologies, which have been proposed as being capable of replacing hard disk drives (HDDs). In this paper, the prospects for these alternative technologies to displace HDDs in 2020 are analyzed. In order to compare technologies, projections were made of storage density and performance in year 2020 for both hard disks and the alternative technologies, assuming the alternative technologies could solve their remaining problems and assuming that hard drives would continue to advance areal density at a pace of about 40% per year, which would result in a two-disk 2.5-in disk drive that stores approximately 40 Terabytes and costs about $40. A major conclusion of the study is that to compete with hard drives on a cost per terabyte basis will be challenging for any solid state technology, because the ITRS lithography roadmap limits the density that most alternative technologies can achieve. Those technologies with the best opportunity have a small cell size and the capability of storing multiple bits per cell. Phase change random access memory (PCRAM) and spin transfer torque random access memory (STTRAM) appear to meet these criteria. PCRAMs are being marketed by at least one supplier and therefore appear to be closer to practical realization. On the other hand, STTRAMs would appear to have a performance edge assuming they, too, can be brought to market with multiple bits per cell. Although there are technologies that are not limited by the lithography roadmap and thus have greater areal density potential, they tend to be further from practical realization.
Performance of Chloroperoxidase Stabilization in Mesoporous Sol-Gel Glass Using In Situ Glucose Oxidase Peroxide Generation (2004)

A unique mesoporous sol-gel glass possessing a highly ordered porous structure (with three pore sizes of about 50, 150, and 200 A diameter) was used as a support material for immobilization of the enzyme chloroperoxidase (CPO). CPO was bound onto the glass via a bifunctional ligand, trimethoxysilylpropanal. In situ production of the cosubstrate, H2O2, was achieved using glucose oxidase. Solvent stability in acetonitrile mixtures was enhanced when a pore size larger than the size of CPO was used (i.e., 200 A). From these results, it appears that the glass-enzyme complex developed through the present work can be used as high-performance biocatalysts for various chemical-processing applications, particularly in harsh conditions.
Neuroprotection by minocycline facilitates significant recovery from spinal cord injury in mice (2003)

Acute spinal cord injury (SCI) produces tissue damage that continues to evolve days and weeks after the initial insult, with corresponding functional impairments. Reducing the extent of progressive tissue loss ('neuroprotection') following SCI should result in a better recovery from SCI, but treatment options have thus far been limited. In this study, we have tested the efficacy of minocycline in ameliorating damage following acute SCI in mice. This semi-synthetic tetracycline antibiotic has been reported to inhibit the expression and activity of several mediators of tissue injury, including inflammatory cytokines, free radicals and matrix metalloproteinases, making it a suitable candidate for study. Mice were subjected to extradural compression of the spinal cord using a modified aneurysm clip, following which they received treatment with either minocycline or vehicle beginning 1 h after injury. Behavioural testing of hindlimb function was initiated 3 days after injury using the Basso Beattie Bresnahan locomotor rating scale, and at 1 week using the inclined plane test. Functional assessments demonstrated that minocycline administration significantly improved both hindlimb function and strength from 3 to 28 days after injury compared with vehicle controls. Furthermore, gross lesion size in the spinal cord was significantly reduced by minocycline, and there was evidence of axonal sparing as determined using fluorogold labelling of the rubrospinal tract and by Bielchowsky silver stain. Finally, a comparison of minocycline against the currently approved treatment for acute SCI in humans, methylprednisolone, demonstrated superior behavioural recovery in the minocycline-treated animals.
High ERp5/ADAM10 expression in lymph node microenvironment and impaired NKG2D ligands recognition in Hodgkin lymphomas (2011)

Herein we describe that in classic Hodgkin lymphomas (cHL, n = 25) the lymph node (LN) stroma displayed in situ high levels of transcription and expression of the disulfide-isomerase ERp5 and of the disintegrin-metalloproteinase ADAM10, able to shed the ligands for NKG2D (NKG2D-L) from the cell membrane. These enzymes were detected both in LN mesenchymal stromal cells (MSCs) and in Reed-Sternberg (RS) cells; in addition, MIC-A and ULBP3 were present in culture supernatants of LN MSCs or RS cells. NKG2D-L-negative RS cells could not be killed by CD8(+)αβT or γδT cells; tumor cell killing was partially restored by treating RS cells with valproic acid, which enhanced NKG2D-L surface expression. Upon coculture with LN MSCs, CD8(+)αβT and γδT cells strongly reduced their cytolytic activity against NKG2D-L(+) targets; this seems to be the result of TGF-β, present at the tumor site, produced in vitro by LN MSCs and able to down-regulate the expression of NKG2D on T lymphocytes. In addition, CD8(+)αβT and γδT cells from the lymph nodes of cHL patients, cocultured in vitro with LN MSCs, underwent TGF-β-mediated down regulation of NKG2D. Thus, in cHL the tumor microenvironment is prone to inhibit the development of an efficient antitumor response.