gebhart/patrepeval huggingface dataset:
Combination of Low-Dose Gemcitabine and Recombinant Quail Vascular Endothelial Growth Factor Receptor-2 as a Vaccine Induces Synergistic Antitumor Activities (2005)

Vascular endothelial growth factor receptor-2 (VEGFR-2) has been shown to play a major role in inducing the full spectrum of VEGF biological response which is essential for tumor angiogenesis. We have demonstrated that immunotherapy of tumors with a vaccine based on quail homologous VEGFR-2 (qVEGFR) was effective in providing both protective and therapeutic antitumor immunity in several tumor models in mice. The purpose of this study was to determine whether the combination therapy of low-dose gemcitabine with qVEGFR as a vaccine could inhibit tumor growth to a greater extent. To test this concept, H22 hepatoma and Lewis lung carcinoma models were established in BALB/c mice and C57BL/6 mice, respectively. Mice were treated with either qVEGFR as a protein vaccine, gemcitabine, or both agents together. qVEGFR or low-dose chemotherapy treatment individually resulted in tumor inhibition to a certain extent.Remarkably, the combination therapy resulted in synergistic antitumor activity. Histological examination revealed that there was endothelial deposition of immunoglobulins within tumor tissues from mice treated with vaccine or combination therapy, especially intratumor angiogenesis was suppressed more significantly for the combination group. Also, ELISPOT analysis showed that mice treated with either qVEGFR alone or in combination with low-dose chemotherapy produced similar amount of anti-VEGFR antibody-producing B cells, which suggested that low-dose gemcitabine did not suppress the host's immune response, but potentiated the antitumor activity of the qVEGFR vaccine. Furthermore, TUNEL staining demonstrated a significant increase in the number of TUNEL-positive cells in the combination group compared with those of other groups. The observations may provide a new bio-chemotherapeutic approach for cancer.
Mesenchymal stem cell-natural killer cell interactions: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2-induced NK-cell proliferation (2005)

In recent years, mesenchymal stem cells (MSCs) have been shown to inhibit T-lymphocyte proliferation induced by alloantigens or mitogens. However, no substantial information is available regarding their effect on natural killer (NK) cells. Here we show that MSCs sharply inhibit IL-2-induced proliferation of resting NK cells, whereas they only partially affect the proliferation of activated NK cells. In addition, we show that IL-2-activated NK cells (but not freshly isolated NK cells) efficiently lyse autologous and allogeneic MSCs. The activating NK receptors NKp30, NKG2D, and DNAM-1 represented the major receptors responsible for the induction of NK-mediated cytotoxicity against MSCs. Accordingly, MSCs expressed the known ligands for these activating NK receptors-ULBPs, PVR, and Nectin-2. Moreover, NK-mediated lysis was inhibited when IFN-gamma-exposed MSCs were used as target cells as a consequence of the up-regulation of HLA class I molecules at the MSC surface. The interaction between NK cells and MSCs resulted not only in the lysis of MSCs but also in cytokine production by NK cells. These results should be taken into account when evaluating the possible use of MSCs in novel therapeutic strategies designed to improve engraftment or to suppress graft-versus-host disease (GVHD) in bone marrow transplantation.
Sensing through the wall imaging using the Army Research Lab ultra-wideband synchronous impulse reconstruction (UWB SIRE) radar (2008)

The U.S. Army Research Laboratory (ARL), as part of a mission and customer funded exploratory program, has developed a new low-frequency, ultra-wideband (UWB) synthetic aperture radar (SAR). The radar is capable of penetrating enclosed areas (buildings) and generating SAR imagery. This supports the U.S. Army's need for intelligence on the configuration, content, and human presence inside these enclosed areas. The radar system is mounted on a ground based vehicle traveling along the road and is configured with an array of antennas pointing toward the enclosed areas of interest. This paper will describe an experiment conducted recently at Aberdeen Proving Ground (APG), Maryland. In this paper we briefly describe the UWB SIRE radar and the test setup in the experiment. We will also describe the signal processing and the image techniques used to produce the SAR imagery. Finally, we will present SAR imagery of the building and its internal structure from different viewing directions.
Electronic Detection of Specific Protein Binding Using Nanotube FET Devices (2003)

We have used nanoscale field effect transistor devices with carbon nanotubes as the conducting channel to detect protein binding. A PEI/PEG polymer coating layer has been employed to avoid nonspecific binding, with attachment of biotin to the layer for specific molecular recognition. Biotin-streptavidin binding has been detected by changes in the device characteristic. Nonspecific binding was observed in devices without the polymer coating, while no binding was found for polymer-coated but not biotinylated devices. Streptavidin, in which the biotin-binding sites were blocked by reaction with excess biotin, produced essentially no change in device characteristic of the biotinylated polymer-coated devices.
On minimal closure constraint generation for symbolic cell assembly (1991)

Article On minimal closure constraint generation for symbolic cell assembly Share on Authors: Debaprosad Dutt AT&T Bell Laboratories, 1247 South Cedar Crest Blvd., Allentown, PA AT&T Bell Laboratories, 1247 South Cedar Crest Blvd., Allentown, PAView Profile , Chi-Yuan Lo AT&T Bell Laboratories, 600 Mountain Avenue, Murray Hill, NJ AT&T Bell Laboratories, 600 Mountain Avenue, Murray Hill, NJView Profile Authors Info & Claims DAC '91: Proceedings of the 28th ACM/IEEE Design Automation ConferenceJune 1991 Pages 736–739https://doi.org/10.1145/127601.127761Online:01 June 1991Publication History 6citation150DownloadsMetricsTotal Citations6Total Downloads150Last 12 Months2Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Plant biochemistry and molecular biology (1997)

Contents : 1. A leaf cell consists of several metabolic compartments -2. The use of energy from sunlight by photosynthesis -3. Photosynthesis is an electron transport process -4. ATP generation by photosynthesis -5. Mitochondria, the power stations of cell -6. Photosynthetic CO2 assimilation by the Calvin cycle -7. Photorespiration -8. Photosynthesis and water consumption -9. Polysaccharides -10. Nitrate assimilation -11. Nitrogen fixation -12. Sulfate assimilation -13. Phloem transport -14. Plant storage proteins -15. Glycerolipids -16. The function of secondary metabolites in plants -17. Isoprenoids -18. Phenylpropanoids -19. Signals regulating the growth and development of plant organs -20. The genomes of plant cells -21. Protein biosynthesis -22. Gene technology in plants.
Occurrence of Propionibacterium freudenreichii bacteriophages in swiss cheese (1995)

We isolated bacteriophages active against Propionibacterium freudenreichii from 16 of 32 swiss cheese samples. Bacteriophage concentrations ranged from 14 to 7 x 10(5) PFU/g, depending on the sample and the sensitive strain used for detection. Only a few strains, 8 of the 44 strains of P. freudenreichii in our collection, were sensitive. We observed that multiplication of bacteriophages occurred in the cheese loaf during multiplication of propionibacteria in a warm curing room, but it seems that these bacteriophages have no adverse effect on the development of the propionic flora. We also found that sensitive cells, originating from either the starter or the cheese-making milk, were present at a high level (10(9) CFU/g) in the cheese.
Efficient Test Generation for Trojan Detection using Side Channel Analysis (2019)

Detection of hardware Trojans is vital to ensure the security and trustworthiness of System-on-Chip (SoC) designs. Side-channel analysis is effective for Trojan detection by analyzing various side-channel signatures such as power, current and delay. In this paper, we propose an efficient test generation technique to facilitate side-channel analysis utilizing dynamic current. While early work on current-aware test generation has proposed several promising ideas, there are two major challenges in applying it on large designs: (i) the test generation time grows exponentially with the design complexity, and (ii) it is infeasible to detect Trojans since the side-channel sensitivity is marginal compared to the noise and process variations. Our proposed work addresses both challenges by effectively exploiting the affinity between the inputs and rare (suspicious) nodes. We formalize the test generation problem as a searching problem and solve the optimization using genetic algorithm. The basic idea is to quickly find the profitable test patterns that can maximize switching in the suspicious regions while minimize switching in the rest of the circuit. Our experimental results demonstrate that we can drastically improve both the side-channel sensitivity (30x on average) and time complexity (4.6x on average) compared to the state-of-the-art test generation techniques.
Crystal and molecular structures of double macrocyclic inclusion complexes composed of cyclodextrins, crown ethers, and cations (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal and molecular structures of double macrocyclic inclusion complexes composed of cyclodextrins, crown ethers, and cationsShigehiro Kamitori, Ken Hirotsu, and Taiichi HiguchiCite this: J. Am. Chem. Soc. 1987, 109, 8, 2409–2414Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April 1987https://pubs.acs.org/doi/10.1021/ja00242a026https://doi.org/10.1021/ja00242a026research-articleACS PublicationsRequest reuse permissionsArticle Views441Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes (2008)

The identification of interaction partners in protein complexes is a major goal in cell biology. Here we present a reliable affinity purification strategy to identify specific interactors that combines quantitative SILAC-based mass spectrometry with characterization of common contaminants binding to affinity matrices (bead proteomes). This strategy can be applied to affinity purification of either tagged fusion protein complexes or endogenous protein complexes, illustrated here using the well-characterized SMN complex as a model. GFP is used as the tag of choice because it shows minimal nonspecific binding to mammalian cell proteins, can be quantitatively depleted from cell extracts, and allows the integration of biochemical protein interaction data with in vivo measurements using fluorescence microscopy. Proteins binding nonspecifically to the most commonly used affinity matrices were determined using quantitative mass spectrometry, revealing important differences that affect experimental design. These data provide a specificity filter to distinguish specific protein binding partners in both quantitative and nonquantitative pull-down and immunoprecipitation experiments.
Ventilation-Induced Chemokine and Cytokine Release Is Associated with Activation of Nuclear Factor- κ B and Is Blocked by Steroids (2001)

Recent clinical trials have shown that the survival of patients with acute respiratory distress syndrome (ARDS) is improved by ventilation with reduced volumes. These studies suggested that overinflation of the lungs causes overactivation of the immune system. The present study investigated the hypothesis that ventilation with increased tidal volumes results in early responses similar to those caused by stimulation with one of the major risk factors for ARDS: bacterial lipopolysaccharide (LPS). We therefore compared the effects of ventilation (-10 cm H2O or -25 cm H2O end-inspiratory pressure) and LPS (50 microg/ml) on nuclear factor (NF)-kappaB activation, chemokine release, and cytokine release in isolated perfused lungs obtained from BALB/C mice. We found that both LPS and ventilation with -25 cm H2O (overventilation; OV) caused translocation of NF-kappaB, which was abolished by pretreatment with the steroid dexamethasone. Furthermore, both treatments resulted in similar increases in perfusate levels of alpha-chemokines (macrophage inflammatory protein; [MIP]-2; KC), beta-chemokines (macrophage chemotactic protein-1; MIP-1alpha), and cytokines (tumor necrosis factor-alpha, interleukin-6), which were largely prevented by dexamethasone pretreatment. In LPS-resistant C3H/HeJ mice, only OV, and not LPS, caused translocation of NF-kappaB and release of MIP-2. We conclude that OV evokes early inflammatory responses similar to those evoked by LPS (i.e., NF-kappaB translocation and release of proinflammatory mediators). The NF-kappaB translocation elicited by OV appears to be independent of Toll-like receptor 4 and not due to LPS contamination introduced by the ventilator. Our data further suggest that steroids might be considered as a subsidiary treatment during artificial mechanical ventilation.
Charge transport inTiO2/MEH−PPVpolymer photovoltaics (2001)

We study the effect of polymer thickness, hole mobility, and morphology on the device properties of polymer-based photovoltaics consisting of MEH-PPV as the optically active layer, ${\mathrm{TiO}}_{2}$ as the exciton dissociation surface, and ITO and Au electrodes. We demonstrate that the conversion efficiency in these polymer-based photovoltaics is primarily limited by the short exciton diffusion length combined with a low carrier mobility. For MEH-PPV devices with optimal device geometry, we achieve quantum efficiencies of 6% at the maximum absorption of the polymer, open circuit voltages of 1.1 V, current densities of 0.4 ${\mathrm{m}\mathrm{A}/\mathrm{c}\mathrm{m}}^{2}$ and rectification ratios greater than ${10}^{5}$ under 100 ${\mathrm{m}\mathrm{W}/\mathrm{c}\mathrm{m}}^{2}$ white light illumination. In addition, we achieve fill factors up to 42% at high light intensities and as high as 69% at low light intensities. We conclude by presenting a model that describes charge transport in ${\mathrm{s}\mathrm{o}\mathrm{l}\mathrm{i}\mathrm{d}\ensuremath{-}\mathrm{s}\mathrm{t}\mathrm{a}\mathrm{t}\mathrm{e}\phantom{\rule{0ex}{0ex}}\mathrm{p}\mathrm{o}\mathrm{l}\mathrm{y}\mathrm{m}\mathrm{e}\mathrm{r}/\mathrm{T}\mathrm{i}\mathrm{O}}_{2}$-based photovoltaics and suggest methods for improving energy conversion efficiencies in polymer-based photovoltaics.
Parallel Personal Comments on “Classical” Papers in Combinatorial Chemistry (1998)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTParallel Personal Comments on "Classical" Papers in Combinatorial ChemistryMichal LeblView Author Information Trega Biosciences, 9880 Campus Point Drive, San Diego, California 92121 Cite this: J. Comb. Chem. 1999, 1, 1, 3–24Publication Date (Web):December 23, 1998Publication History Received12 November 1998Published online23 December 1998Published inissue 12 January 1999https://pubs.acs.org/doi/10.1021/cc9800327https://doi.org/10.1021/cc9800327review-articleACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views1144Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Combinatorial chemistry,Genetics,Mixtures,Monomers,Peptides and proteins Get e-Alerts
In vitro adhesion of Escherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors (1978)

Escherichia coli strains with pili (K99 or 987P) known to facilitate intestinal colonization adhered in vitro to porcine intestinal epithelial cells. These strains adhered equally to both ileal and jejunal epithelial cells. A laboratory E. coli strain that has type 1 pili also adhered to porcine intestinal epithelial cells. When nonpiliated cells derived from 987P+, K99+, or type 1 pilus+ strains were used for in vitro adhesion assays, they failed to adhere. The attachment of piliated bacteria to epithelial cells was a saturable process that plateaued at 30 to 40 bacterial cells attached per epithelial cell. Competitive inhibition of bacterial cell attachment to epithelial cells with purified pili showed that only purified 987P competed against the 987P+ strain and only purified type 1 pili competed against the type 1 pilus+ strain. Competition between a K99+ strain and K99 was not consistently achieved. K99+, 987P+, and type 1 pilus+ bacteria could be prevented from adhering to epithelial cells by Fab fragments specific for K99, 987P, or type 1 pili, respectively. Fab fragments specific for non-K99 bacterial surface antigens did not inhibit adhesion of the K99+ strain. It is concluded that adhesion of E. coli to porcine intestinal epithelial cells in vitro is mediated by pili and that the epithelial cells used apparently had different receptors for different pili.
Recombinant Viral Vector Vaccines for the Veterinary Use. (1997)

Recently, genetically engineering using recombinant DNA techniques has been applied to design new viral vaccines in order to reduce some problems which present viral vaccines have. Up to now, many viruses have been investigated for development of recombinant attenuated vaccines or live viral vectors for delivery of foreign immunogenic antigens. In this review, we introduced three kind of viruses; herpesviruses, vaccinia viruses, and adenoviruses, which have best widely been studied as recombinant vaccines or delivery vaccines for the veterinary use.
Narrowband interference suppression in time-hopping impulse-radio systems (2003)

Ultra-wideband (UWB) radio systems have drawn a lot of attention during the last few years. These systems use very low transmission power, spread over a bandwidth of several gigahertz. The very low transmission power and the large bandwidth used enable UWB radio systems to coexist with other narrowband systems over the same frequency band without interfering with the narrowband systems. Nevertheless, these narrowband systems may cause interference which jams the UWB receiver completely. Since standard narrowband interference suppression techniques are not applicable, techniques for interference suppression have to be developed. This paper presents novel narrowband interference suppression algorithms for UWB radio systems. Theoretical analysis of these algorithms reveal that they can eliminate the narrowband interference almost completely.
Analysis of the complex transcription termination region of the Escherichia coli rrn B gene (1991)

The complex terminator region of the Escherichia coli rrnB gene was analyzed by subcloning the terminators T1 and T2 and the inverted repeats IR1 and IR2 individually, or in various combinations, in a normal or inverted orientation into a terminator probe vector. The in vivo terminating efficiency was assayed by measuring the galactokinase activity encoded by the downstream galK gene. Termination efficiencies of all fragments were compared in two constructs, differing in the presence or absence of readthrough translation over the investigated terminator signal. The following main conclusions were drawn. (a) T1 and T2 are both efficient terminators in isolated forms. (b) IR1 and IR2 have some terminating effect (much lower than the proper terminators), especially in the inverted orientation. Their presence modifies the effect of the proper terminators in a quite unpredictable way, especially if these regions are translated. (c) The terminators are not symmetrical; in the inverted orientation T1 is practically inactive and T2 termination is reduced. (d) Translation radically decreases the efficiency of the terminators. (e) Several sequences in the rrnB gene, upstream of the terminator region (one in the 16S RNA and one in the 5S RNA coding region), are very efficient in vivo terminators in the inverted orientation.
A versatile high speed 250‐MHz pulse imager for biomedical applications (2008)

A versatile 250 MHz pulse electron paramagnetic resonance (EPR) instrument for imaging of small animals is presented. Flexible design of the imager hardware and software makes it possible to use virtually any pulse EPR imaging modality. A fast pulse generation and data acquisition system based on general purpose PCI boards performs measurements with minimal additional delays. Careful design of receiver protection circuitry allowed us to achieve very high sensitivity of the instrument. In this article we demonstrate the ability of the instrument to obtain three dimensional images using the electron spin echo (ESE) and single point imaging (SPI) methods. In a phantom that contains a 1 mM solution of narrow line (16 μT, peak-to-peak) paramagnetic spin probe we achieved an acquisition time of 32 seconds per image with a fast 3D ESE imaging protocol. Using an 18 minute 3D phase relaxation (T(2e)) ESE imaging protocol in a homogeneous sample a spatial resolution of 1.4 mm and a standard deviation of T(2e) of 8.5% were achieved. When applied to in vivo imaging this precision of T(2e) determination would be equivalent to 2 torr resolution of oxygen partial pressure in animal tissues.
Lithocholic acid disrupts phospholipid and sphingolipid homeostasis leading to cholestasis in mice (2011)

This study revealed that LCA induced disruption of phospholipid/sphingolipid homeostasis through TGF-β signaling and that serum LPC is a biomarker for biliary injury.