gebhart/patrepeval huggingface dataset:
RANKING OF WORKING FLUIDS FOR ORGANIC RANKINE CYCLE APPLICATIONS (2004)

Renewed interest in waste heat power recovery has resulted in the development of Organic Rankine Cycle (ORC) products with the recently commercialized refrigerant R-245fa [1,2,3]. A thermodynamic cycle analysis program predicting the performance of ORC systems has been written in support of the development of these products. This cycle program allows the use of different organic working fluids and various sources of waste heat. It also accounts for actual turbine and pump efficiencies, refrigerant line pressure losses and finite boiler and condenser surface area. Using this cycle program, the relative thermodynamic efficiency of various potential working fluids is determined for three waste heat power recovery applications: gas engine exhaust, landfill flare gas and condensing steam. The analysis is carried out for both recuperated and non-recuperated ORC cycle configurations and includes watercooled as well as air-cooled condenser options. The differences in ORC thermodynamic cycle efficiency of various refrigerants were found to be substantially larger than the differences in thermodynamic efficiency of these same fluids for vapor compression cycle applications. Second law analysis of the results explains these findings. ORC thermodynamic efficiency correlates well with the critical temperature of the working fluid. Future ORC systems could benefit from the development of safe and environmentally acceptable working fluids with higher critical temperatures than currently available.
T Cell Receptor Peptide Therapy Triggers Autoregulation of Experimental Encephalomyelitis (1991)

Encephalitogenic T cells specific for myelin basic protein share common V beta 8 peptide sequences in their T cell receptor (TCR) that can induce autoregulatory T cells and antibodies that prevent clinical signs of experimental autoimmune encephalomyelitis (EAE). It is not known, however, if TCR peptides can treat established disease. To test its therapeutic value, TCR-V beta 8-39-59 peptide was injected into rats with clinical signs of EAE. This treatment reduced disease severity and speeded recovery, apparently by boosting anti-V beta 8 T cells and antibodies raised naturally in response to encephalitogenic V beta 8+ T cells. These results demonstrate that synthetic TCR peptides can be used therapeutically, and implicate the TCR-V beta 8-39-59 sequence as a natural idiotope involved in EAE recovery. Similarly, human TCR peptides may be effective in enhancing natural regulation of autoreactive T cells that share common V genes.
P nucleotide insertions and the resolution of hairpinDNA structures in mammalian cells. (1994)

Two lines of evidence point to a hairpin DNA intermediate in V(D)J joining (V, variable; D, diversity; J, joining) [Lieber, M.R. (1991) FASEB J. 4, 2934-2944]. One is the presence of P nucleotide insertions (short inverted-repeat sequence) in V(D)J junctions [Lafaille, J. J., DeCloux, A., Bonneville, M., Takagaki, Y. & Tonegawa, S. (1989) Cell 59, 859-870]; a second is the detection of site-specifically broken DNA molecules with covalently closed (hairpin) termini in thymus DNA [Roth, D. B., Menetski, J. P., Nakajima, P., Bosma, M. J. & Gellert, M. (1989) Cell 70, 983-991]. However, P nucleotide insertions could be generated in ways not involving a hairpin structure, and because physical evidence for hairpin-ended DNA fragments has been obtained only with mutant mice, there is some uncertainty regarding the role of hairpin molecules in the normal V(D)J joining pathway. To determine whether mammalian cells are capable of metabolizing this odd type of DNA terminus and whether, in doing so, junctions with P insertions are in fact created, a linear DNA molecule with a hairpin closure at each end was transfected into several murine cell lines. The hairpin-ended molecules were recircularized, and the junctions exhibited P insertions at a high frequency. This result directly links the presence of P insertions to a hairpin precursor, providing strong evidence for the notion that a hairpin DNA intermediate exists in V(D)J recombination. A comparison of hairpin end joining in various cells, including those derived from mice with the severe combined immunodeficiency (scid) mutation, is presented.
Encapsulated Key Escrow (1996)

The main objection to current key-escrow proposals is that they assume complete faith in the authority and its trustees. If the authority does not follow the rules, or is replaced by an un-trustworthy authority tomorrow, it can immediately recover the secret keys of all users, and embark on massive wiretapping. We introduce a new approach tokey escrow called encapsulated key escrow (EKE). With this approach itis computationally possible for an authority to wiretap individual users, but computationally prohibitive for the authority to launch large scale wiretapping. This is achieved by imposing a time delay between obtaining the escrowed information of a user and actually recovering the secret key. Furthermore, the recoverability is veri able at escrow time. The approach is applicable both for session keys and for public key cryptography. EKE is a simple general paradigm, applicable across cryptosystems and key distribution protocols, regardless of their type. It solves in one stroke the problem of imposing time delays in key escrow. In particular it yields the rst time delayed key escrow system for RSA, and more e cient solutions for Di e-Hellman than achievable by the previous approach to time delays, namely partial key escrow (PKE). The idea behind EKE is a new cryptographic tool called a veri able cryptographic time capsule (VCTC). This has broader applications to \\sending information into the future.&amp;amp;quot;
Activation of B cells in vivo by a Fab/Fc fragment of a monoclonal anti-IgD antibody requires an interaction between the antibody fragment and a cellular IgG Fc receptor. (1988)

To investigate activation of B lymphocytes in vivo by an interaction between B cell surface Ig (sIg) and an anti-Ig antibody, we compared the abilities of a divalent IgG2b anti-IgD mAb, H delta a/1, and its univalent Fab/Fc fragment to enhance B cell sIa expression in vivo. The Fab/Fc fragment consists of a single Fab linked to Fc, and can interact with C and cellular Fc receptors. Although injection of BALB/c mice with either intact H delta a/1 or H delta a/1 Fab/Fc enhanced splenic B cell sIa expression, Ia expression was enhanced more by intact H delta a/1. Furthermore, injection of mice with 24G2, a mAb to the B cell and macrophage IgG2b Fc receptor, completely blocked the ability of 20 to 500 micrograms of H delta a/1 Fab/Fc to enhance B cell sIa expression, but had no effect on enhancement of B cell sIa expression by 100 micrograms of intact H delta a/1. This effect of 24G2 was mediated by its blocking of IgG2b receptor function rather than simply by its binding to B lymphocytes, since a mAb to the B cell IgE receptor did not interfere with the ability of H delta a/1 Fab/Fc to enhance B cell sIa expression. The different effects of 24G2 on B cell activation by intact H delta a/1 and H delta a/1 Fab/Fc were not a result of differences in the abilities of the intact antibody and its Fab/Fc fragment to activate B cells, since 24G2 did not interfere with the ability of AMS-15, a IgG2a anti-IgD mAb, to slightly increase B cell sIa expression. These observations indicate that a univalent ligand for B cell sIg can activate B lymphocytes in vivo through an IgGFc-IgGFc receptor-dependent interaction.
Ultraviolet-Induced Cell Death Blocked by a Selenoprotein from a Human Dermatotropic Poxvirus (1998)

Selenium, an essential trace element, is a component of prokaryotic and eukaryotic antioxidant proteins. A candidate selenoprotein homologous to glutathione peroxidase was deduced from the sequence of molluscum contagiosum, a poxvirus that causes persistent skin neoplasms in children and acquired immunodeficiency syndrome (AIDS) patients. Selenium was incorporated into this protein during biosynthesis, and a characteristic stem-loop structure near the end of the messenger RNA was required for alternative selenocysteine decoding of a potential UGA stop codon within the open reading frame. The selenoprotein protected human keratinocytes against cytotoxic effects of ultraviolet irradiation and hydrogen peroxide, providing a mechanism for a virus to defend itself against environmental stress.
HUMHOT: a database of human meiotic recombination hot spots (2005)

Meiotic recombination occurs preferentially at certain regions in the genome referred to as hot spots. The number of hot spots known in humans has increased manifold in recent years. The identification of these hot spots in humans is of great interest to population and medical geneticists since they influence the structure of Linkage Disequilibrium and Haplotype blocks in human populations, whose patterns have applications in mapping disease genes. HUMHOT is a web-based database of Human Meiotic Recombination Hot Spots. The database comprises DNA sequences corresponding to the hot spot regions from the literature that have been mapped to a high resolution (<4 kb) in humans. It also provides flanking sequence information for the hot spot region along with references describing the hot spot. The database can be queried based on hot spot identity, chromosome position or by homology to user-defined sequences. It is also updated with new hot spot sequences as they are discovered and provides hyperlinks to commonly used tools for estimating recombination rates, performing genetic analysis and new advances in our understanding of meiotic hot spots. Public access to the HUMHOT database is available at http://www.jncasr.ac.in/humhot.
Pyridones as Highly Selective, Noncovalent Inhibitors of T790M Double Mutants of EGFR (2015)

The rapid advancement of a series of noncovalent inhibitors of T790M mutants of EGFR is discussed. The optimization of pyridone 1, a nonselective high-throughput screening hit, to potent molecules with high levels of selectivity over wtEGFR and the broader kinome is described herein.
Reliability study of 850 nm VCSELs for data communications (1996)

We present reliability results for 850 nm Vertical Cavity Surface Emitting Laser (VCSEL) devices. Hundreds of parts from multiple wafer lots and package styles were subjected to burn-in for thousands of hours at various temperatures and currents, yielding more than one million device-hours of data. Possible failure mechanisms are also discussed.
Heteromer Induction: An Approach to Unique Pharmacology? (2017)

It is proposed that two types of opioid receptor heteromers exist: a) those that are constitutive and b) those that are induced by bivalent ligands. Mu opioid agonists interact with constitutive MOR-DOR heteromer to mediate tolerance and dependence. Bivalent ligand, MDAN21, is devoid of these adverse effects by virtue of its DOR antagonist pharmacophore. We propose that bivalent ligands MMG22 and MCC22 induce colocalized receptors to form heteromers (MOR-mGluR5 and MOR-CCR5, respectively) that do not occur naturally, thereby promoting unique pharmacology. Heteromer induction with bivalent ligands offers a general approach to unique pharmacology that complements traditional SAR.
Preconditioning donor liver withNodosinperfusion lessens rat ischemia reperfusion injury via heme oxygenase‐1 upregulation (2011)

This study suggests that preconditioning with Nodosin perfusion provides a potential protective effect through inducing HO-1 expression to attenuate ischemia/reperfusion injury in liver transplantation.
Magnetic Energy Harvesting and Concentration at a Distance by Transformation Optics (2012)

Based on transformation optics, we introduce a magnetic shell with which one can harvest magnetic energy and distribute it as desired in space with unprecedented efficiency at an arbitrary scale. It allows a very large concentration of magnetic energy in a free space region, which can be used for increasing the sensitivity of magnetic sensors, and the transfer of magnetic energy from a source to a given distant point separated by empty space, with possible applications in wireless transmission of energy.
DEG/ENaC channels: A touchy superfamily that watches its salt (1999)

To the surprise of many, studies of molecular mechanisms of touch transduction and analyses of epithelial Na+ transport have converged to define a new class of ion channel subunits. Based on the names of the first two identified subfamilies, the Caenorhabditis elegans degenerins and the vertebrate epithelial amiloride-sensitive Na+ channel, this ion channel class is called the DEG/ENaC superfamily. Members of the DEG/ENaC superfamily have been found in nematodes, flies, snails, and vertebrates. Family members share common topology, such that they span the membrane twice and have intracellular N- and C-termini; a large extracellular loop includes a conserved cysteine-rich region. DEG/ENaC channels have been implicated a broad spectrum of cellular functions, including mechanosensation, proprioception, pain sensation, gametogenesis, and epithelial Na+ transport. These channels exhibit diverse gating properties, ranging from near constitutive opening to rapid inactivation. We discuss working understanding of DEG/ENaC functions, channel properties, structure/activity correlations and possible evolutionary relationship to other channel classes.
Using the two-hybrid system to detect protein-protein interactions (1993)

Abstract Protein-protein interactions are essential in almost all biological processes, including replication, transcription, secretion, signal transduction, and metabolism. Thus a central question in the study of any protein is to determine what other proteins are in contact with it. The answer, in the case of oncogene-encoded proteins for example, has provided enormous insights into the problems of cell cycle control and differentiation. It is, therefore, clear that intense research efforts will continue to focus on identifying proteins that interact with some protein of interest, referred to here as the target protein. Current approaches to detect interacting proteins include traditional methods such as co-immunoprecipitation, crosslinking, and copurification through gradients or chromatographic columns (see Midgley and Lane, Chapter 6 of this volume). These biochemical methods have the major disadvantage that interacting proteins are generally known only as bands of a particular relative mobility on a polyacrylamide gel. To progress from these bands to cloned genes is often a difficult undertaking, involving such methods as the initial purification of sufficient protein for amino acid sequencing or antibody production, followed by additional work to screen a library for the corresponding.
Plant antitumor agents. 25. Total synthesis and antileukemic activity of ring A substituted camptothecin analogs. Structure-activity correlations (1987)

Nineteen racemic ring A substituted analogues of the antitumor agent 20(S)-camptothecin were prepared by total synthesis and evaluated for in vitro cytotoxic activity against KB cell culture and in vivo antileukemic activity against L1210. These compounds bore a wide variety of substituents at C11 designed to confer upon the ring system a broad range of combinations of electronic, steric, and lipophilic effects. A few C10-substituted derivatives as well as C10,C11-disubstituted analogues prepared as part of a concurrent study have also been included for general comparison. With the notable exception of the cyano derivative, the 11-substituted compounds displayed only modest in vitro and in vivo activities, and there was a remarkable insensitivity toward the nature of the substituent. In contrast, the 9- and 10-substituted compounds exhibited a considerably higher level of dose potency and activity both in vitro and in vivo.
Microarrays and toxicology: The advent of toxicogenomics (1999)

The availability of genome-scale DNA sequence information and reagents has radically altered life-science research. This revolution has led to the development of a new scientific subdiscipline derived from a combination of the fields of toxicology and genomics. This subdiscipline, termed toxicogenomics, is concerned with the identification of potential human and environmental toxicants, and their putative mechanisms of action, through the use of genomics resources. One such resource is DNA microarrays or "chips," which allow the monitoring of the expression levels of thousands of genes simultaneously. Here we propose a general method by which gene expression, as measured by cDNA microarrays, can be used as a highly sensitive and informative marker for toxicity. Our purpose is to acquaint the reader with the development and current state of microarray technology and to present our view of the usefulness of microarrays to the field of toxicology.
Orthogonal Self-Assembly of Carbon Nanotube Crossbar Architectures by Simultaneous Graphoepitaxy and Field-Directed Growth (2006)

Crossbar arrays of single-wall carbon nanotubes are produced spontaneously in a single step of chemical vapor deposition by simultaneous graphoepitaxy along faceted nanosteps and field-directed growth, perpendicular to each other. The two alignment mechanisms take place selectively on miscut C-plane sapphire and patterned amorphous SiO2 islands, respectively, without mutual interference, producing dense nanotube grids, with up to 12 junctions per square micrometer. This one-step method of orthogonal self-assembly may open up new possibilities for nanotube circuit integration.
Oncolytic therapy using a mutant type-1 herpes simplex virus and the role of the immune system (1999)

We conclude that HSV-1716 may provide a oncolytic therapy for lung cancer even in the absence of immune system induction and a "carrier" cell could potentially deliver this vector.
Generation of Functional Hepatocyte-Like Cells from Human Pluripotent Stem Cells in a Scalable Suspension Culture (2013)

Recent advances in human embryonic and induced pluripotent stem cell-based therapies in animal models of hepatic failure have led to an increased appreciation of the need to translate the proof-of-principle concepts into more practical and feasible protocols for scale up and manufacturing of functional hepatocytes. In this study, we describe a scalable stirred-suspension bioreactor culture of functional hepatocyte-like cells (HLCs) from the human pluripotent stem cells (hPSCs). To promote the initial differentiation of hPSCs in a carrier-free suspension stirred bioreactor into definitive endoderm, we used rapamycin for "priming" phase and activin A for induction. The cells were further differentiated into HLCs in the same system. HLCs were characterized and then purified based on their physiological function, the uptake of DiI-acetylated low-density lipoprotein (LDL) by flow cytometry without genetic manipulation or antibody labeling. The sorted cells were transplanted into the spleens of mice with acute liver injury from carbon tetrachloride. The differentiated HLCs had multiple features of primary hepatocytes, for example, the expression patterns of liver-specific marker genes, albumin secretion, urea production, collagen synthesis, indocyanin green and LDL uptake, glycogen storage, and inducible cytochrome P450 activity. They increased the survival rate, engrafted successfully into the liver, and continued to present hepatic function (i.e., albumin secretion after implantation). This amenable scaling up and outlined enrichment strategy provides a new platform for generating functional HLCs. This integrated approach may facilitate biomedical applications of the hPSC-derived hepatocytes.
Growth and Assembly of Crystalline Tungsten Oxide Nanostructures Assisted by Bioligation (2005)

Here, we present a systematic study on the influence of the bioligand deferoxamine mesylate on the crystallization and assembly behavior of tungsten oxide in a soft-chemistry process. Without deferoxamine mesylate, this approach yields pseudo-single crystalline tungstite nanoplatelets consisting of a large number of crystallographically almost perfectly aligned primary crystallites. In the presence of a constant amount of deferoxamine, the particle morphology drastically changes with temperature, ranging from wormlike organic-inorganic hybrid nanostructures to single-crystalline tungsten oxide nanowires, highlighting the role of the bioligand in controlling the crystal growth and assembly behavior. The nanowires have a uniform diameter of about 1.3 nm, an aspect ratio of more than 500, and the structural flexibility of tungsten oxide. The presented process is based on the combination of biomimetic construction principles with nonaqueous sol-gel chemistry, thus combining the advantages of both tools, excellent control over particle morphology and high crystallinity at low temperature.
Low-level laser therapy effects in traumatized permanent teeth with extrusive luxation in an orthodontic patient (2010)

The aim of this case report was to present and evaluate the effect of low-level laser therapy on traumatized permanent teeth with extrusive luxation in an orthodontic patient. The treatment and follow-up evaluation of two orally luxated maxillary permanent central incisors in a 19-year-old man is described. Detailed anamnesis was taken, and extraoral, intraoral, radiographic examinations and electrical and thermal pulpal tests were performed to determine the type of the luxation and the further treatment protocol. Teeth were splinted with composite resin, and antibiotic therapy was prescribed. Low-level laser therapy was applied for 25 sessions. No root canal treatment was applied to the teeth. Continuation of the orthodontic treatment was restarted after 6 months. No sign of clinical or radiographic pathology was detected after 2 years from the end of the treatment. Teeth were identified healthy and sound without any root canal intervention. Treatments with low-level laser applications may be evaluated as noninvasive alternative treatment options in comparison with endodontic treatment for teeth with extrusive luxation more than 2 mm, especially for those who have orthodontic treatment needs.
Mapping and Mutational Analysis of the IgE‐Binding Epitopes on Ara h 1, a Legume Vicilin Protein and a Major Allergen in Peanut Hypersensitivity (1997)

Peanut allergy is a significant health problem because of the prevelance and potential severity of the allergic reaction. Serum IgE from patients with documented peanut hypersensitivity reactions and overlapping peptides were used to identify the IgE-binding epitopes on the major peanut allergen, Ara h 1. At least twenty-three different linear IgE-binding epitopes, located throughout the length of the Ara h 1 protein, were identified. All of the epitopes were 6-10 amino acids in length, but there was no obvious sequence motif shared by all peptides. Four of the peptides appeared to be immunodominant IgE-binding epitopes in that they were recognized by serum from more than 80% of the patients tested and bound more IgE than any of the other Ara h 1 epitopes. Mutational analysis of the immunodominant epitopes revealed that single amino acid changes within these peptides had dramatic effects on IgE-binding characteristics. The identification and determination of the IgE-binding capabilities of core amino acids in epitopes on the Ara h 1 protein will make it possible to address the pathophysiologic and immunologic mechanisms regarding peanut hypersensitivity reactions specifically and food hypersensitivity in general.