gebhart/patrepeval huggingface dataset:
High Density Lipoprotein (HDL) Reduces Renal Ischemia/Reperfusion Injury (2003)

High-density lipoproteins (HDL) have been shown to reduce organ injury and mortality in animal models of shock via modulation of the expression of adhesion molecules and pro-inflammatory enzymes. As renal inflammation plays an important role in the development of ischemia/reperfusion (I/R) injury of the kidney, the aim of this study was to investigate the ability of HDL to alleviate renal dysfunction and injury in a rat model of renal I/R. HDL (80 mg/kg, intravenous) was administered to male Wistar rats 30 min before bilateral renal ischemia for 45 min followed by reperfusion for up to 48 h. After 6-h reperfusion, HDL significantly reduced (1) renal and tubular dysfunction, (2) tubular and reperfusion-injury, and (3) histologic evidence of renal injury. HDL also improved renal function (after 24-h and 48-h reperfusion) and reduced histologic signs of renal injury (after 48-h reperfusion). Administration of HDL significantly reduced the numbers of polymorphonuclear leukocytes (PMN) infiltrating into renal tissues during reperfusion, which was reflected by an attenuation of the increase in renal myeloperoxidase activity caused by I/R. Furthermore, HDL markedly reduced expression of the adhesion molecules, intercellular adhesion molecule-1 (ICAM-1), and P-selectin during reperfusion. The increase in renal malondialdehyde levels caused by renal I/R was also significantly reduced by HDL, suggesting attenuation of lipid peroxidation subsequent to oxidative stress. These results demonstrate that HDL significantly reduces renal I/R injury and severity of ischemic acute renal failure. It is proposed that the mechanism of protection involves reduction of the expression of adhesion molecules, resulting in reduction of PMN infiltration and oxidative stress.
Physicochemical Characterization and Dissolution Properties of Meloxicam-Gelucire 50/13 Binary Systems (2011)

A solid dispersion of Meloxicam (MX), a poorly soluble, non steroidal anti-inflammatory drug, and Gelucire 50/13 was prepared by spray drying. Spherical microparticles were yielded with smooth surfaces as observed by scanning electron microscopy. According to differential scanning calorimetry and powder X-ray diffractometry analysis, MX was transformed from the crystalline state to the amorphous state as confirmed by the disappearance of its melting peak and the crystalline peaks. The dissolution tests at pH 7.4 revealed that the dissolution rate of encapsulated MX was 2.5-fold higher than that of the corresponding physical mixture and fourfold higher than the drug alone, respectively. The microparticles prepared at a ratio of 1:4 (drug/Gelucire) exhibited a 4-fold higher anti-inflammatory activity on the paw edema of rats in comparison to the drug alone. All in all, this work reveals that spray drying is a suitable technique for preparation of solid dispersions with improved biopharmaceutical and pharmacological characteristics of MX.
Two Dimensional Electronic Spectroscopy (2003)

Two different electronically resonant two-dimensional spectroscopies are described. The first, two-color photon echo peak shift spectroscopy, is sensitive to correlations in transition frequency between the initial and probed (final) states. It provides new insight into the mechanism of ultrafast solvation and should prove useful for characterizing dynamics in inhomogeneous systems in general. The second technique, fifth order threepulse scattering, contains two coherence periods whose durations are controlled. The entire two-dimensional surface was recorded for a dye molecule in dilute solution and a photosynthetic light-harvesting complex. The data provide insight into the short-time dynamics of solvation and exciton relaxation, respectively.
Molecular Profile of Peripheral Blood Mononuclear Cells from Patients with Rheumatoid Arthritis (2007)

Rheumatoid arthritis (RA) is a chronic inflammatory arthritis. Currently, diagnosis of RA may take several weeks, and factors used to predict a poor prognosis are not always reliable. Gene expression in RA may consist of a unique signature. Gene expression analysis has been applied to synovial tissue to define molecularly distinct forms of RA; however, expression analysis of tissue taken from a synovial joint is invasive and clinically impractical. Recent studies have demonstrated that unique gene expression changes can be identified in peripheral blood mononuclear cells (PBMCs) from patients with cancer, multiple sclerosis, and lupus. To identify RA disease-related genes, we performed a global gene expression analysis. RNA from PBMCs of 9 RA patients and 13 normal volunteers was analyzed on an oligonucleotide array. Compared with normal PBMCs, 330 transcripts were differentially expressed in RA. The differentially regulated genes belong to diverse functional classes and include genes involved in calcium binding, chaperones, cytokines, transcription, translation, signal transduction, extracellular matrix, integral to plasma membrane, integral to intracellular membrane, mitochondrial, ribosomal, structural, enzymes, and proteases. A k-nearest neighbor analysis identified 29 transcripts that were preferentially expressed in RA. Ten genes with increased expression in RA PBMCs compared with controls mapped to a RA susceptibility locus, 6p21.3. These results suggest that analysis of RA PBMCs at the molecular level may provide a set of candidate genes that could yield an easily accessible gene signature to aid in early diagnosis and treatment.
Regional glucose metabolic abnormalities are not the result of atrophy in Alzheimer's disease (1998)

Reduced glucose metabolism measured by PET in AD is not simply an artifact due to an increase in CSF space induced by atrophy, but reflects a true metabolic reduction per gram of tissue.
Mobile robot localization by tracking geometric beacons (1991)

The application of the extended Kaman filter to the problem of mobile robot navigation in a known environment is presented. An algorithm for, model-based localization that relies on the concept of a geometric beacon, a naturally occurring environment feature that can be reliably observed in successive sensor measurements and can be accurately described in terms of a concise geometric parameterization, is developed. The algorithm is based on an extended Kalman filter that utilizes matches between observed geometric beacons and an a priori map of beacon locations. Two implementations of this navigation algorithm, both of which use sonar, are described. The first implementation uses a simple vehicle with point kinematics equipped with a single rotating sonar. The second implementation uses a 'Robuter' mobile robot and six static sonar transducers to provide localization information while the vehicle moves at typical speeds of 30 cm/s.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Patterning cells and their environments using multiple laminar fluid flows in capillary networks (1999)

This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.
Identification and characterization of a gene that is coamplified with dihydrofolate reductase in a methotrexate-resistant CHO cell line. (1989)

As part of an effort to characterize the spatial and functional relationships among genetic elements within the amplified dihydrofolate reductase (DHFR) domain in Chinese hamster cells, we have used a variation of the differential hybridization approach to identify cDNA clones whose genes are coamplified with DHFR in the methotrexate-resistant cell line, CHOC 400. Our initial screen was successful in isolating both DHFR and non-DHFR cDNAs. One of the non-DHFR cDNA clones, 2BE2121, hybridizes on Northern (RNA) blots to abundant 1,200- and 1,500-nucleotide (nt) transcripts which differ in the lengths of their 3' untranslated regions. The clone 2BE2121 contains a 789-nt open reading frame but does not appear to be related to any members of the protein or nucleic acid sequence databases. A second larger non-DHFR cDNA, II-19-211, was isolated that is transcribed from the same gene as 2BE2121 but contains only a small carboxyl-terminal portion of the open reading frame. II-19-211 may, therefore, represent either a splicing intermediate or an mRNA transcribed from a cryptic intragenic promoter. Hybridization to cosmids from the DHFR domain shows that 2BE2121 is encoded by a gene approximately 34 kilobases (kb) long. The 5'-most genomic fragment is less than 4 kb from an interamplicon junction. The 3' end of the 2BE2121 gene lies approximately 75 kb downstream from the DHFR gene and approximately 25 kb downstream from the proximal replication initiation site, and the transcriptional polarity is opposite to that of the leading strand of replication. Thus, both the DHFR and 2BE2121 genes are exceptions to the theory that transcription proceeds in the same direction as the leading strand of the replication fork.
Actions of C-type natriuretic peptide and sodium nitroprusside on carbachol-stimulated inositol phosphate formation and contraction in ciliary and iris sphincter smooth muscles. (1997)

In bovine CM, SNP effects are probably mediated through soluble guanylyl cyclase, whereas in Sph the CNP effects are mediated through membrane-bound guanylyl cyclase, which is associated with the type-B natriuretic peptide receptor. Agents that strongly increase intracellular cGMP levels, including SNP and CNP, produce significant inhibition of CCh-induced IP3 production and contraction. These effects are tissue and species specific. The results indicate that the cGMP signaling system, similar to the cAMP system, has a major inhibitory influence on the muscarinic responses in smooth muscles of the iris-ciliary body. The agents CNP and SNP, which stimulate cGMP accumulation in the ocular smooth muscles, could reduce intraocular pressure, presumably by increasing uveoscleral outflow induced by relaxation of the CM. However, the relationships between the CNP- and SNP-induced inhibition of the muscarinic stimulation and the reported intraocular pressure-lowering effects of the cGMP-elevating agents remain to be determined.
Towards very large aperture massive MIMO: A measurement based study (2014)

Massive MIMO is a new technique for wireless communications that claims to offer very high system throughput and energy efficiency in multi-user scenarios. The cost is to add a very large number of antennas at the base station. Theoretical research has probed these benefits, but very few measurements have showed the potential of Massive MIMO in practice. We investigate the properties of measured Massive MIMO channels in a large indoor venue. We describe a measurement campaign using 3 arrays having different shape and aperture, with 64 antennas and 8 users with 2 antennas each. We focus on the impact of the array aperture which is the main limiting factor in the degrees of freedom available in the multiple antenna channel. We find that performance is improved as the aperture increases, with an impact mostly visible in crowded scenarios where the users are closely spaced. We also test MIMO capability within a same user device with user proximity effect. We see a good channel resolvability with confirmation of the strong effect of the user hand grip. At last, we highlight that propagation conditions where line-of-sight is dominant can be favourable.
Surface-Confined Living Radical Polymerization for Coatings in Capillary Electrophoresis (1998)

Surface-confined living radical polymerization is shown to be a controlled means of covalently bonding both linear and cross-linked polymer films on silica. CuCl/bipyridine initiates radical formation through atom transfer with a self-assembled monolayer of benzyl chloride, onto which polymer then grows. The polymerization is intrinsically confined to the surface, avoiding problems associated with polymer formed in the solution. The surface-confined polymerization scheme is generally applicable to radical polymerization of vinyl monomers and was studied here for the case of acrylamide. Infrared spectroscopy shows that the film growth is controllable, and atomic force microscopy reveals that smooth films are prepared. The surface-confinement polymerization scheme was tested for both linear and cross-linked polyacrylamide. Capillary electrophoresis of strongly basic proteins confirms that the coated capillaries provide the high efficiency expected for polyacrylamide. The cross-linked coating exhibits higher reproducibility with respect to migration time than does the linear coating. Surface-confined living radical polymerization prepares linear and cross-linked polymer films without danger of clogging narrow capillaries and will ultimately facilitate cross-laboratory comparisons by enabling control of film thickness.
Synthesis of Poly(L-lactic acid-co-L-lysine) Graft Copolymers (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Poly(L-lactic acid-co-L-lysine) Graft CopolymersJeffrey S. Hrkach, Jean Ou, Noah Lotan, and Robert LangerCite this: Macromolecules 1995, 28, 13, 4736–4739Publication Date (Print):June 1, 1995Publication History Published online1 May 2002Published inissue 1 June 1995https://pubs.acs.org/doi/10.1021/ma00117a055https://doi.org/10.1021/ma00117a055research-articleACS PublicationsRequest reuse permissionsArticle Views886Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Importance of apoptosis in the histopathology of drug related lesions in the large intestine. (1993)

The presence of crypt apoptoses in substantial numbers (with an apoptotic count in excess of 5) should always raise the possibility of drug effect. The mechanisms involved are not clear but with NSAIDs the changes might well be immunologically mediated.
"Allergen-specific immunotherapy utilizing mechanisms for immune regulation" (2008)

In Europe and America, the allergen immunotherapy (AIT) for various hay fevers or allergic rhinitis is enforced as causal treatment methods. However, in Japan, the only AIT is subcutaneous immunotherapy (SCIT) for Japanese cedar pollinosis and not so popular due to the long term treatment and the obscure mechanism of action being elucidated etc. We are now advancing research of the new vaccine technologies which aim at the practical applications of ASIT to Japanese cedar pollenosis. The first candidate vaccine is immunoregulatory liposomes which include antigenic polypeptides and chemicals for the induction of immunoregulatory cells such as invariant natural killer T (iNKT) cells, regulatory dendritic cells and regulatory T (Treg) cells. The liposomes encapsulated ovalbumin (OVA) were prepared and injected intraperitoneally into mice primed with alum-adsorbed OVA. After subsequent challenge with OVA alone, IgE and IgG antibody responses were remarkably suppressed for several months. The results suggest that the immunoregulatory liposomes containing antigenic polypeptides might suppress on-going IgE antibody formations during the pollen-season and induce long-term immune tolerance after the treatment. To elucidate the mechanism of action, next, the spleen cells of the mice treated with the immunoregulatory liposomes were analyzed. As a result, the liposomes were taken into B cells such as marginal zone B (MZB) cells in addition to the dendritic cells or the macrophages. It is demonstrated that IL-10 production after the interaction of the liposomes-captured B cells with iNKT cells are involved in the induction of Treg cells. Now, as a vaccine of Japanese cedar pollenosis, the immunoregulatory liposomes encapsulating the designed recombinant Cryj 1-Cryj 2 fusion protein is manufactured so that there may be no risk of anaphylaxis. In a mouse model sensitized with natural Cry j1 and Cry j2 antigens, the vaccine showed the suppression of IgE and IgG antibody responses after the challenge with the antigens. Moreover, oral administration of the vaccine also showed the efficacy for the IgE antibody suppression. Taken together, it is suggested that our basic technology for immunoregulatory liposomes can be applicable for any allergen-specific immunotherapies.
Purification and characterization of N‐acyl‐D‐glutamate deacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A‐6 (1991)

The purification and properties of N ‐acyl‐D‐glutamate deacylase from the cell extracts of Alcaligenes xylosoxydans subsp. xylosoxydans A‐6 were studied. The two active fractions (peaks I and II) were obtained by a Mono Q column chromatography. The predominant enzyme (peak I) has been purified, 1960‐fold to homogeneity and characterized. The enzyme was a monomer with a molecular weight of 59 000. The optimum pH and the isoelectric point were 8.0 and 5.5, respectively. The enzyme catalyzed the hydrolysis of N ‐acyl derivatives of D‐glutamate. The K m s for N ‐acetyl, N ‐butyryl and N ‐propionyl derivatives of D‐glutamate were 0.129, 0.066 and 0.01 mM, respectively.
The management of urinary incontinence in the male neurological patient (2014)

Standard options for treatment of urinary incontinence in neurological patients remain largely unchanged. Alternative treatment options, especially of conservative or minimally invasive character, have the potential to further broaden the therapeutic spectrum. While a higher level of evidence is needed to assess the potential of such therapeutic approaches, randomized controlled trials in the male neurological population present a challenge. To truly advance treatment of urinary continence in male neurological patients, well-designed, multicenter studies are warranted.
An Adhesin of the Yeast Pathogen Candida glabrata Mediating Adherence to Human Epithelial Cells (1999)

Candida glabrata is an important fungal pathogen of humans that is responsible for about 15 percent of mucosal and systemic candidiasis. Candida glabrata adhered avidly to human epithelial cells in culture. By means of a genetic approach and a strategy allowing parallel screening of mutants, it was possible to clone a lectin from a Candida species. Deletion of this adhesin reduced adherence of C. glabrata to human epithelial cells by 95 percent. The adhesin, encoded by the EPA1 gene, is likely a glucan-cross-linked cell-wall protein and binds to host-cell carbohydrate, specifically recognizing asialo-lactosyl-containing carbohydrates.
Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation (2009)

The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA sequencing, single-nucleotide polymorphism (SNP) analysis, DNA chips, DNA single-strand conformation polymorphism (SSCP) analysis and many other techniques. The purity and yield of ssDNA can affect the success of each application. This study compares the two ssDNA production methods, the strand separation by streptavidin-coated magnetic beads and alkaline denaturation and the lambda exonuclease digestion, in regard to the purity of generated ssDNA and the efficiency. Here, we demonstrate the considerable benefits of ssDNA production by lambda exonuclease digestion for in vitro selection of DNA aptamers. We believe that the generation of ssDNA aptamers using this method will greatly improve the success rate of SELEX experiments concerning the recovery of target-specific aptamers.
Oncogenic GNAQ mutations are not correlated with disease-free survival in uveal melanoma (2009)

The GNAQ mutation status is not suitable to predict DFS. However, the high frequency of GNAQ mutations may render it a promising target for therapeutic intervention.
Long-term follow-up of psilocybin-assisted psychotherapy for psychiatric and existential distress in patients with life-threatening cancer (2020)

These findings suggest that psilocybin-assisted psychotherapy holds promise in promoting long-term relief from cancer-related psychiatric distress. Limited conclusions, however, can be drawn regarding the efficacy of this therapy due to the crossover design of the parent study. Nonetheless, the present study adds to the emerging literature base suggesting that psilocybin-facilitated therapy may enhance the psychological, emotional, and spiritual well-being of patients with life-threatening cancer.
Photodynamic therapy in the management of resistant lower urinary tract carcinoma (1987)

Twenty-three patients with resistant transitional cell carcinoma (TCC) of the bladder and posterior urethra had photodynamic therapy (PDT). Seventy-two hours after an intravenous injection with 2 mg/kg of the photosensitizer dihematoporphyrin ether (DHE) (Photofrin II, Photomedica, Raritan, NJ), each patient received cystoscopic light treatment. The light dose to the whole bladder using the bulb diffuser ranged from 5 to 60 J/cm2; power density ranged from 9 to 22 mW/cm2. The focal light dose ranged from 100 to 200 J/cm2 at a power density from 100 to 200 mW/cm2. To treat the urethra, a diffuser fiber was used to deliver 200 to 400 J/linear cm at a power of 110 to 300 mW/cm. In the 19 of 23 patients who were treated for resistant superficial TCC, 83.3% of the tumors had responded at the initial follow-up evaluation. Seven patients had a complete response and 10 had a partial response. Three of 19 patients who received inadequate light treatment failed to respond. Control of intractable gross hematuria was the only benefit for patients with locally invasive disease (greater than or equal to T2). Irritating lower urinary tract symptoms that varied in severity and duration occurred in all patients. Four patients experienced bladder shrinkage, which did not resolve. Although the light and drug doses remain to be determined, a whole bladder light dose of 15 to 20 J/cm2 with a drug dose of 2 mg/kg seems to be effective enough without producing permanent adverse effects in the bladder.