gebhart/patrepeval huggingface dataset:
Compartmentalized roles for leukocytic adhesion molecules in lung inflammatory injury. (1995)

By using the model of acute injury caused by intrapulmonary deposition of IgG immune complexes, blocking mAb to CD11a, CD11b, L-selectin, and intercellular adhesion molecule-1 (ICAM-1) were administered either i.v. or intratracheally (i.t.). The effects of these interventions were assessed according to lung injury, lung content of myeloperoxidase (MPO), TNF-alpha, and cellular content in bronchoalveolar lavage (BAL) fluids, and up-regulation of pulmonary vascular ICAM-1. In animals treated i.v. with Abs to CD11a, L-selectin, or ICAM-1 lung injury was significantly attenuated in parallel with reduced lung content of MPO. Under similar conditions, treatment with anti-CD11b had no effect. However, when the same mAb were administered i.t., anti-CD11a and anti-L-selectin were without protective effects, whereas i.t. administered anti-CD11b and anti-ICAM-1 were each highly protective. The protective effects of anti-CD11b were related to profound reductions in BAL levels of TNF-alpha, pulmonary vascular up-regulation of ICAM-1, and lung content of MPO. The protective effects of i.t.-administered anti-ICAM-1 were not associated with reduced BAL levels of TNF-alpha. Protective effects of mAb were also reflected in reductions of retrievable neutrophils in BAL fluids. mAb to rat CD11b and CD18 but not to rat CD11a suppressed in vitro production of TNF-alpha by immune complex-stimulated rat alveolar macrophages. The mAb did not reduce NO2-/NO3- generation in stimulated macrophages but all mAb (except anti-ICAM-1) reduced O2- responses in macrophages. These data suggest a compartmentalized role for adhesion molecules in lung inflammatory injury after intraalveolar deposition of IgG immune complexes, with CD11a, L-selectin, and ICAM-1 being important in the vascular compartment for neutrophil recruitment, whereas in the alveolar compartment CD11b and ICAM-1 (but not CD11a and L-selectin) seem to play key roles.
A novel method for DNA molecular counting (2000)

We have developed a new method for counting DNA molecules using 'capillary-plates' consisting of a large number of small glass-capillary 'channels' fused together in parallel. PCR mixtures containing serially diluted DNA templates with the DNA indicator dye Hoechst 33258 were poured into the plates and sealed with silicone rubber-plates. Following 40 PCR cycles, fluorescence microscopy revealed that the fluorescence in some channels had increased about three-times more than in others at template concentrations of 1 fM or lower. No bright fluorescence was observed in the absence of template. The relationship between the proportion of fluorescent channels in the capillary-plates and the template concentrations was linear according to Poisson probabilities in the range of 0.1-1,000 aM. These results demonstrate the amplification of single templates in the channels, and that a small number of templates could be quantified by counting the proportion of positive channels on the capillary-plates.
<title>New 0.54 aperture i-line wafer stepper with field-by-field leveling combined with global alignment</title> (1991)

i-line wafer steppers are evolving as established production tools, and it is evident that they will be used to realize features in the sub-half-micron region. Consequently, i-line steppers can be expected to be the equipment of choice for volume production of 16 MBit DRAMs and possibly the first generation of 64 Mbit devices, before the introduction of DUV lithography. However, for this sub-half-micron resolution, lenses with higher apertures and large field sizes will be required. In this paper a new family of wafer steppers is introduced, with a new mechanical frame design and modular architecture which can accommodate a family of large field i-line and deep UV lenses. Results from the first lens type with NAequals0.54 and a field diameter of 25.5 mm are described. To overcome the anticipated depth of focus problems on production wafers, a field-by-field leveling system is introduced, ensuring optimum focus over the whole image field. A challenging problem of wafer steppers using this option is the alignment accuracy during stepping of stage and active leveling of the wafer chuck. The stepper concept introduced here is able to realize the field-by-field leveling without the need for the throughput consuming field-by-field alignment. For that purpose a wafer stage with a new metrology system and improved accuracy has been designed, resulting in an overlay accuracy better than 85 nm in the global alignment mode. Simultaneously a throughput of more than 80 150 mm wafers per hour is realized. Along with the new lens and metrology concept, the stepper contains local environmental control systems performing better than class 1, to ensure clean handling of 8-inch wafers without the need for space consuming environmental enclosures. This paper reports practical results from the new stepper, including resist features below 0.4 micrometers , overlay measurements, particle control, as well as a general description of the new stepper architecture.
Offline recognition of unconstrained handwritten texts using HMMs and statistical language models (2004)

This paper presents a system for the offline recognition of large vocabulary unconstrained handwritten texts. The only assumption made about the data is that it is written in English. This allows the application of Statistical Language Models in order to improve the performance of our system. Several experiments have been performed using both single and multiple writer data. Lexica of variable size (from 10,000 to 50,000 words) have been used. The use of language models is shown to improve the accuracy of the system (when the lexicon contains 50,000 words, the error rate is reduced by approximately 50 percent for single writer data and by approximately 25 percent for multiple writer data). Our approach is described in detail and compared with other methods presented in the literature to deal with the same problem. An experimental setup to correctly deal with unconstrained text recognition is proposed.
Co- and post-translational processing of the hevein preproprotein of latex of the rubber tree (Hevea brasiliensis) (1991)

Hevein is a chitin-binding protein of 43 amino acids found in the lutoid body-enriched fraction of rubber tree latex. A hevein cDNA clone (HEV1) (Broekaert, W., Lee, H.-i., Kush, A., Nam, C.-H., and Raikhel, N. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 7633-7637) encodes a putative signal sequence of 17 amino acids followed by a polypeptide of 187 amino acids. Interestingly, this polypeptide has two distinct domains: an amino-terminal domain of 43 amino acids, corresponding to mature hevein, and a carboxyl-terminal domain of 144 amino acids. To investigate the mechanisms involved in processing of the protein encoded by HEV1, three domain-specific antisera were raised against fusion proteins harboring the amino-terminal domain (N domain), carboxyl-terminal domain (C domain), and both domains (NC domain). Translocation experiments using an in vitro translation system show that the first 17-amino acid sequence encoded by the cDNA functions as a signal peptide. Immunoblot analysis of proteins extracted from lutoid bodies demonstrates that a 5-kDa protein comigrated with purified mature hevein and cross-reacted with N domain- and NC domain-specific antibodies. A 14-kDa protein was recognized by C domain- and NC domain-specific antibodies. A 20-kDa protein was cross-reactive with all three antibodies. Microsequencing data further suggest that the 5-kDa (amino-terminal domain) and 14-kDa (carboxyl-terminal domain) proteins are post-translational cleavage products of the 20-kDa polypeptide (both domains) which corresponds to the proprotein encoded by HEV1. In addition, it was found that the amino-terminal domain could provide chitin-binding properties to a fusion protein bearing it either amino terminally or carboxyl terminally.
Neocortical neuron number in humans: Effect of sex and age (1997)

Modern stereological methods provide precise and reliable estimates of the number of neurons in specific regions of the brain. We decided to estimate the total number of neocortical neurons in the normal human brain and to analyze it with respect to the major macro- and microscopical structural components, to study the internal relationships of these components, and to quantitate the influence of important physiological variables on brain structure. The 94 brains reported represent a consecutive collection of brains from the general Danish population. The average numbers of neocortical neurons were 19 billion in female brains and 23 billion in male brains, a 16% difference. In our study, which covered the age range from 20 years to 90 years, approximately 10% of all neocortical neurons are lost over the life span in both sexes. Sex and age were the main determinants of the total number of neurons in the human neocortex, whereas body size, per se, had no influence on neuron number. Some of the data presented have been analyzed by using new mathematical designs. An equation predicting the total neocortical neuron number in any individual in which sex and age are known is provided.
Expression of Integrin ανβ3 in Small Blood Vessels of Glioblastoma Tumors (1996)

Angiogenesis, which promotes tumor proliferation and invasiveness, is mediated by integrin-dependent cell adhesion mechanisms and requires expression of integrin alpha v beta 3. To determine whether integrin alpha v beta 3 is expressed in the small blood vessel hyperplasia characteristic of glioblastoma tumors, we explored the mRNA and protein expression of integrin alpha v, beta 3, beta 5, and beta 1 subunits in small blood vessels in gliomas of various grades and nontumorous brain biopsies. Antisense beta 3 and beta 5 riboprobes hybridized to small blood vessels (endothelial and adjacent mesenchymal cells) in 11 of 11 glioblastomas and 2 of 4 anaplastic astrocytomas, but failed to hybridize to small blood vessels in 12 non-neoplastic specimens and 4 low-grade astrocytomas. In contrast, antisense alpha v and beta 1 riboprobes hybridized to small blood vessels in all of the biopsies. The expression of integrin alpha v beta 3 protein in these vessels was determined by immunohistochemical analysis using anti-integrin subunit-specific antibodies. The findings were similar to those obtained using in situ hybridization: beta 3 subunit protein was detected on small blood vessels in 9 of 12 glioblastoma and 2 of 4 anaplastic astrocytomas, whereas it was not detected in small blood vessels of 10 non-neoplastic brains and 4 low-grade astrocytomas. In contrast, the alpha v and beta 1 subunit proteins were detected in small blood vessels in all of the brain biopsies, whereas the beta 5 subunit protein could not be detected. These data suggest that integrin alpha v beta 3 is expressed in small blood vessels of glioblastoma tumors.
Cyclopolycondensations. VIII. New Thermally Stable Aromatic Polybenzoxazinones by Solution Polymerization in Polyphosphoric Acid (1967)

Abstract High molecular weight polybenzoxazinones have been prepared by cyclo-polycondensation reaction of 4,4′-diamino-3,3′-biphenyldicarboxylic acid with a variety of aromatic carbonyl compounds using a solution polymerization technique in polyphosphoric acid. From the model reactions of anthranilic acid, and 4,4′-diamino-3,3′-biphenyldicarboxylic acid with benzoyl chloride in polyphosphoric acid, it is established that the cyclopolycondensation proceeds through the formation of an open-chain tractable precursor, polyamic acid of high molecular weight (ninh = 2.66) in the first step, which subsequently undergoes thermal or chemical cyclodehydration along the polymer chain, to yield, in the second step, a fully aromatic polybenz-oxazinone. Polybenzoxazinones thus obtained have excellent thermal stability both in nitrogen and in air. The optimum polymerization conditions for obtaining polyamic acid of high molecular weight are determined by the study of reaction variables such as polymerization temperatures, monomer concentrations, and reaction time as well as the effect of P2O5 concentrations in polyphosphoric acid.
The beta-interferon promoter responds to priming through multiple independent regulatory elements. (1994)

The induction of beta-interferon is markedly enhanced in some cell types by a pretreatment with type I interferon itself (priming). We show that induction in response to double-stranded RNA is completely dependent upon priming in HeLa cells. However, unprimed cells can be partially induced by Sendai virus. This indicates that Sendai virus can provide a signal that is different from, or additional to, that provided by double-stranded RNA. The requirement for priming cannot be localized to a specific sequence element in the beta-interferon promoter, suggesting that priming may induce an essential component for signal transduction in response to double-stranded RNA.
Techniques for improving the performance of CELP-type speech coders (1992)

Techniques for improving the performance of CELP (code excited linear prediction)-type speech coders while maintaining reasonable computational complexity are explored. A harmonic noise weighting function, which enhances the perceptual quality of the processed speech, is introduced. The combination of harmonic noise weighting and subsample pitch lag resolution significantly improves the coder performance for voiced speech. Strategies for reducing the speech coder's data rate, while maintaining speech quality, are presented. These include a method for efficient encoding of the long-term predictor lags, utilization of multiple gain vector quantizers, and a multimode definition of the speech coder frame. A 5.9-kb/s VSELP speech coder that incorporates these features is described. Complexity reduction techniques which allow the coder to be implemented using a single fixed-point DSP (digital signal processor) are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Two methods of large-scale extraction of an antibiotic produced by Myxococcus coralloides. (1979)

Two methods for the isolation of an antibiotic produced by Myxococcus coralloides have been developed: the chloroform extraction method and the charcoal adsorption method. The recovery of antibiotic in each case was 25% and 30%, respectively, by the two methods. Although the two methods yield a relative low recovery, the charcoal adsorption method seems more attractive and promising due to its simplicity and economic advantages.
Performance evaluation and demonstration of light-trails in shared wavelength optical networks (SWONs) (2005)

Light-trails are a promising candidate for grooming of traffic in the optical layer. We investigated the performance of light-trails via theory, simulation and experiment focusing on its efficiency. (2 pages)
Discovery of 1,5-Benzodiazepines with Peripheral Cholecystokinin (CCK-A) Receptor Agonist Activity (II):  Optimization of the C3 Amino Substituent (1996)

Analogs of the previously reported 1,5-benzodiazepine peripheral cholecystokinin (CCK-A) receptor agonist 1 were prepared which explore substitution and/or replacement of the C-3 phenyl urea moiety. Agonist efficacy on the isolated guinea pig gallbladder (GPGB) was retained with a variety of substituted ureas and amide analogs. Three compounds were identified which were orally active in the mouse gallbladder emptying assay (MGBE). The 2-indolamide (52) and N-(carboxymethyl)-2-indolamide (54) derivatives had improved affinity for the human CCK-A receptor but reduced agonist efficacy on the GPGB. Neither indolamide was orally active in a rat feeding assay. In contrast, the (3-carboxyphenyl)urea derivative (29, GW7854) had moderately increased affinity for the human CCK-B receptor but was a potent full agonist on the GPGB and was orally active in both the MGBE and rat feeding assays. GW7854 was a full agonist (EC50 = 60 nM) for calcium mobilization on CHO K1 cells expressing hCCK-A receptors and a potent antagonist of CCK-8 (pA2 = 9.1) on CHO K1 cells expressing hCCK-B receptors. GW7854 is a potent mixed CCK-A agonist/CCK-B antagonist which is orally active in two in vivo models of CCK-A-mediated agonist activity.
FIELD EVALUATION OF A RESPIRATORY SYNCYTIAL VIRUS VACCINE AND A TRIVALENT PARAINFLUENZA VIRUS VACCINE IN A PEDIATRIC POPULATION1 (1969)

Journal Article FIELD EVALUATION OF A RESPIRATORY SYNCYTIAL VIRUS VACCINE AND A TRIVALENT PARAINFLUENZA VIRUS VACCINE IN A PEDIATRIC POPULATION Get access JAMES CHIN, JAMES CHIN Calif. State Dep't. Public HealthBerkeley 94704 Reprint requests to Dr. Chin, Calif. State Dept.of Public Health; or to the Vaccine Development Branch, N.I.A.I.D., N.I.H., Bethesda, Md. 20014 Search for other works by this author on: Oxford Academic PubMed Google Scholar ROBERT L. MAGOFFIN, ROBERT L. MAGOFFIN Calif. State Dep't. Public HealthBerkeley 94704 Search for other works by this author on: Oxford Academic PubMed Google Scholar LOIS ANN SHEARER, LOIS ANN SHEARER Calif. State Dep't. Public HealthBerkeley 94704 Search for other works by this author on: Oxford Academic PubMed Google Scholar JACK H. SCHIEBLE, JACK H. SCHIEBLE Calif. State Dep't. Public HealthBerkeley 94704 Search for other works by this author on: Oxford Academic PubMed Google Scholar EDWIN H. LENNETTE EDWIN H. LENNETTE Calif. State Dep't. Public HealthBerkeley 94704 Search for other works by this author on: Oxford Academic PubMed Google Scholar American Journal of Epidemiology, Volume 89, Issue 4, April 1969, Pages 449–463, https://doi.org/10.1093/oxfordjournals.aje.a120957 Published: 01 April 1969 Article history Received: 08 August 1968 Published: 01 April 1969
An R-Type Ca2+Current in Neurohypophysial Terminals Preferentially Regulates Oxytocin Secretion (1999)

Multiple types of voltage-dependent Ca(2+) channels are involved in the regulation of neurotransmitter release (Tsien et al., 1991; Dunlap et al., 1995). In the nerve terminals of the neurohypophysis, the roles of L-, N-, and P/Q-type Ca(2+) channels in neuropeptide release have been identified previously (Wang et al., 1997a). Although the L- and N-type Ca(2+) currents play equivalent roles in both vasopressin and oxytocin release, the P/Q-type Ca(2+) current only regulates vasopressin release. An oxytocin-release and Ca(2+) current component is resistant to the L-, N-, and P/Q-type Ca(2+) channel blockers but is inhibited by Ni(2+). A new polypeptide toxin, SNX-482, which is a specific alpha(1E)-type Ca(2+) channel blocker (Newcomb et al., 1998), was used to characterize the biophysical properties of this resistant Ca(2+) current component and its role in neuropeptide release. This resistant component was dose dependently inhibited by SNX-482, with an IC(50) of 4.1 nM. Furthermore, SNX-482 did not affect the other Ca(2+) current types in these CNS terminals. Like the N- and P/Q-type Ca(2+) currents, this SNX-482-sensitive transient Ca(2+) current is high-threshold activated and shows moderate steady-state inactivation. At the same concentrations, SNX-482 blocked the component of oxytocin, but not of vasopressin, release that was resistant to the other channel blockers, indicating a preferential role for this type of Ca(2+) current in oxytocin release from neurohypophysial terminals. Our results suggest that an alpha(1E) or "R"-type Ca(2+) channel exists in oxytocinergic nerve terminals and, thus, functions in controlling only oxytocin release from the rat neurohypophysis.
A Double‐Blind, Placebo‐Controlled Pilot Study to Evaluate the Efficacy and Safety of Oral Nalmefene HCI for Alcohol Dependence (1994)

A dozen studies have been published showing that opiate antagonists suppress alcohol drinking in animals, and two independent placebo-controlled, double-blind clinical trials of naltrexone found this agent was associated with decreased alcohol craving and consumption in alcohol-dependent patients. Nalmefene is a newer opiate antagonist that has a number of potential advantages over naltrexone in the treatment of alcoholism, including no dose-dependent association with liver toxicity and more effective binding to central opiate receptors. Consequently, a double-blind pilot study was conducted to gather preliminary data on the safety and efficacy of nalmefene for reducing alcohol consumption in alcohol-dependent subjects. Twenty-one alcohol-dependent subjects meeting admission criteria were randomly assigned to 12 weeks of double-blind treatment with 40 mg nalmefene, 10 mg nalmefene, or placebo, resulting in 7 patients/treatment group. Nalmefene was well tolerated, with no serious adverse drug reactions. The 40 mg group had a significantly lower rate of relapse (p < or = 0.05), and a greater increase in the number of abstinent days/week (p < or = 0.09), than the other treatment groups. A significant decrease in the number of drinks/drinking day was noted for both nalmefene groups (p < or = 0.04), but not for placebo. These results were supported by parallel decreases in ALT. These pilot data provide preliminary support for the hypotheses that nalmefene can be safely given to alcoholics, and that nalmefene may have a role in reducing alcohol consumption and preventing relapse, particularly at the 40 mg level. A full-scale study is underway to confirm these preliminary findings.
Development of a Method for the Quantification of 1α,25(OH)2–Vitamin D3 in Serum by Liquid Chromatography Tandem Mass Spectrometry without Derivatization (2010)

Quantification of 1alpha,25(OH)(2)-vitamin D(3) in serum is technically challenging because of its very low concentration. Even mass spectrometry faces a challenge due to the low sensitivity for this molecule, unless a specific derivatization is implemented. Therefore, there is still a need for a robust, simplified, sensitive and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the quantification of 1alpha,25(OH)(2)-vitamin D(3). Thanks to tandem mass- spectrometry associated to an on-line sample extraction and clean-up, sample preparation has been reduced to a simple protein precipitation step and derivatization has been avoided. Specimen volume has been kept to a minimum. The innovative aspects of the hereby-presented method are the use of stable lithium adducts and a highly sensitive tandem mass spectrometer. The achieved limit of quantification is at a level of 15 pg mL(-1), with a % CV of 5-15% at physiological concentration levels. The linearity is good and spiking experiments yielded a recovery of 87-102%. Comparison of selected samples with an established reference method, using an extensive purification procedure (high-performance liquid chromatography and radioimmunoassay), has shown a good correlation.