gebhart/patrepeval huggingface dataset:
A new approach to photoalignment: photo-addition to a self-assembled monolayer (2008)

Photoalignment used to control the orientation of nematic liquid crystals in LCDs usually involves the photochemical modification of the chemical structure of an organic thin film so that an optical and/or steric anisotropy is developed. We now demonstrate a novel photoadditive alternative whereby small photoreactive molecules are bound to a photoreactive SAM surface by irradiation with polarised UV so that the surface properties are changed and a surface anisotropy is developed.
Recombinase-Mediated Gene Activation and Site-Specific Integration in Mammalian Cells (1991)

A binary system for gene activation and site-specific integration, based on the conditional recombination of transfected sequences mediated by the FLP recombinase from yeast, was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequence to activate an otherwise silent beta-galactosidase reporter gene. Clones of marked cells were generated by excisional recombination within a chromosomally integrated copy of the silent reporter. By the reverse reaction, integration of transfected DNA was targeted to a specific chromosomal site. The results suggest that FLP could be used to mosaically activate or inactivate transgenes for analysis of vertebrate development, and to efficiently integrate transfected DNA at predetermined chromosomal locations.
The Definition and Unit of Ionic Strength (2001)

The term ionic strength is defined differently in different textbooks and monographs. Some define it in terms of mol kg-1, while others define it in terms of the non-SI unit mol L-1. The conversion to the SI unit mol m3 creates a problem in computing mean ionic activity coefficients. A dimensionless ionic strength unit has also appeared. These various definitions make the concept of ionic strength very confusing to students, especially in the use of this quantity to calculate mean activity coefficients through the Debye-Hückel theory. To resolve this confusion, a recommendation is given to define ionic strength as a dimensionless quantity.
Image Analysis and Computer Simulation of Nanoparticle Clustering in Combustion Systems (2009)

The current work presents a new method to model and characterize the formation and growth of nanoparticles clusters which includes the three dimensional geometric properties of the clusters, such as aspect ratios, main chain length, radius of gyration, fractal dimension and fractal prefactor. In the model, semi-stochastic mathematics is used to represent varying levels of Coulomb and van der Waals forces during clustering of monodisperse particles. Parametric studies of the relative particle interactions are conducted to evaluate the effects on cluster geometry. Radius of gyration and main chain length were identified as the geometric properties with the greatest sensitivity to changes in the interparticle forces. To demonstrate the analytical capability of the approach, clusters of combustion-generated tin dioxide (SnO2) nanoparticles were imaged and evaluated to identify the dominant forces controlling cluster morphology. The results show the geometry of the SnO2 clusters is a result of strong Coulomb interactions.
The C‐terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity (2008)

A new crystal structure of the dimeric cysteine synthase CysM from Mycobacterium tuberculosis reveals an open and a closed conformation of the enzyme. In the closed conformation the five carboxy-terminal amino acid residues are inserted into the active site cleft. Removal of this segment results in a decreased lifetime of the alpha-aminoacrylate reaction intermediate, an increased sensitivity to oxidants such as hydrogen peroxide, and loss of substrate selectivity with respect to the sulfur carrier thiocarboxylated CysO. These results highlight features of CysM that might be of particular importance for cysteine biosynthesis under oxidative stress in M. tuberculosis.
Detection of Tumor Marker CA125 in Ovarian Carcinoma Using Quantum Dots (2004)

Semiconductor quantum dots (QDs) offer several advantages over organic dyes in fluorescence-imaging applications, such as higher quantum yield, exceptional photostability, and a narrow, tunable, and symmetric emission spectrum. To explore whether QDs could specifically and effectively label tumor markers and be used in immunohistochemistry as a novel type of fluorescent probe, we used quantum dots with maximum emission wavelength 605 nm (QD605) to detect the ovarian carcinoma marker CA125 in specimens of different types (fixed cells, tissue sections, and xenograft piece). Additionally, we compared the photostability of QD signals with that of a conventional organic dye, FITC. All labeling signals of QDs were found to be more specific and brighter than those of FITC. Moreover, the QDs exhibited exceptional photostability during continuous illumination for 1 h by a high-intensity laser (Ar laser power 100 mW) at 488 nm, while the FITC signals faded very quickly and became undetectable after 24 min of illumination. These results indicate that QD-based probes can offer substantial advantages over existing fluorophores in many applications, and can be used effectively in immunohistochemistry as a novel class of fluorescent probes.
In vivo control of redox potential during protein folding catalyzed by bacterial protein disulfide-isomerase (DsbA). (1993)

The formation of disulfide bonds in Escherichia coli is catalyzed by periplasmic protein disulfide-isomerase (DsbA). When the alpha-amylase/trypsin inhibitor from Ragi, a protein containing five intramolecular disulfide bridges, is secreted into the periplasm of E. coli, large amounts of misfolded inhibitor with incomplete or incorrect disulfides are accumulated. Folding of the inhibitor in the periplasm is not improved when DsbA is coexpressed and cosecreted. However, an up to 14-fold increase in correctly folded inhibitor is observed by co-expression of DsbA in conjugation with the addition of reduced glutathione to the growth medium. This peptide acts as a disulfide-shuffling reagent and can pass the outer membrane of E. coli. Since the influence of DsbA on the folding yield of the inhibitor is reduced in the presence of oxidized glutathione, the in vivo function of DsbA appears to be dependent on the ratio between oxidizing and reducing thiol equivalents in the periplasm. The high stability of thiol reagents against air oxidation during growth of E. coli allows the investigation of oxidative protein folding in vivo under controlled, thiol-dependent redox conditions.
Minocycline versus Doxycycline in the Treatment of Lyme Neuroborreliosis (2000)

Figure 1. Ratio of CSF: serum concentrations of doxycycline and minocycline after multiple 100-mg oral doses given twice daily. Data are from [11].
Cyclosporine improves psoriasis in a double-blind study (1986)

In a double-blind trial, 21 patients with severe plaque psoriasis were randomly assigned to receive oral cyclosporine, 14 mg/kg/d, or its vehicle. After four weeks of therapy the 11 cyclosporine recipients had the following response to treatment: two had total clearing and six improved markedly, two moderately, and one minimally; whereas ten vehicle-treated patients showed no change or minimal improvement. Vehicle-treated patients, after a switch to cyclosporine for four weeks, demonstrated impressive improvement similar to that seen in patients who initially received only cyclosporine. Moderate or marked improvement or total clearing was noted in 17 (81%) of 21 and 20 (95%) of 21 after one and four weeks of therapy, respectively. Mitotic figures and leukotriene B4 levels in lesions decreased 86% and 64%, respectively, after seven days of cyclosporine therapy. Mononuclear (including activated T cells) and polymorphonuclear leukocyte infiltrates were markedly reduced in lesions of all patients after seven days of cyclosporine therapy. These results suggest that (1) psoriasis may have an immunologic basis mediated by activated T cells and/or other immune cells; (2) if a long-term regimen with a favorable efficacy—side effect ratio can be determined, cyclosporine would be a significant advance in the treatment of psoriasis. (<i>JAMA</i>1986;256:3110-3116)
Color Analysis of Facial Skin: Detection of Emotional State (2014)

Humans show emotion through different channels such as facial expression, head poses, gaze patterns, bodily gestures, and speech prosody, but also through physiological signals such as skin color changes. The concentration levels of hemoglobin and blood oxygenation under the skin vary due to changes in a person's emotional and physical state, this produces subtle changes in the hue and saturation components of their skin color. In this paper, we present an evaluation of facial skin color changes as the only feature to infer the emotional state of a person. We created a dataset of spontaneous human emotions with a wide range of human subjects of different ages and ethnicities. We used three different types of video clips as stimuli: negative, neutral, and positive to elicit emotions on subjects. We performed experiments using various machine learning algorithms including decision trees, multinomial logistic regression and latent-dynamic conditional random field. Our preliminary results show that facial skin color changes can be used to infer the emotional state of a person in the valence dimension with an accuracy of 77.08%.
Epicardial lead implantation techniques for biventricular pacing via left lateral mini-thoracotomy, video-assisted thoracoscopy, and robotic approach. (2003)

Epicardial lead implantation for biventricular pacing is feasible with all 3 surgical techniques. Each method allows optimal lead implantation under direct vision and therefore reduces the incidence of nonresponders resulting from suboptimal lead placement.
On the Modulatory Roles of Neuregulins/ErbB Signaling on Synaptic Plasticity (2019)

Neuregulins (NRGs) are a family of epidermal growth factor-related proteins, acting on tyrosine kinase receptors of the ErbB family. NRGs play an essential role in the development of the nervous system, since they orchestrate vital functions such as cell differentiation, axonal growth, myelination, and synapse formation. They are also crucially involved in the functioning of adult brain, by directly modulating neuronal excitability, neurotransmission, and synaptic plasticity. Here, we provide a review of the literature documenting the roles of NRGs/ErbB signaling in the modulation of synaptic plasticity, focusing on evidence reported in the hippocampus and midbrain dopamine (DA) nuclei. The emerging picture shows multifaceted roles of NRGs/ErbB receptors, which critically modulate different forms of synaptic plasticity (LTP, LTD, and depotentiation) affecting glutamatergic, GABAergic, and DAergic synapses, by various mechanisms. Further, we discuss the relevance of NRGs/ErbB-dependent synaptic plasticity in the control of brain processes, like learning and memory and the known involvement of NRGs/ErbB signaling in the modulation of synaptic plasticity in brain's pathological conditions. Current evidence points to a central role of NRGs/ErbB receptors in controlling glutamatergic LTP/LTD and GABAergic LTD at hippocampal CA3-CA1 synapses, as well as glutamatergic LTD in midbrain DA neurons, thus supporting that NRGs/ErbB signaling is essential for proper brain functions, cognitive processes, and complex behaviors. This suggests that dysregulated NRGs/ErbB-dependent synaptic plasticity might contribute to mechanisms underlying different neurological and psychiatric disorders.
8 Gb 3-D DDR3 DRAM Using Through-Silicon-Via Technology (2010)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> An 8 Gb 4-stack 3-D DDR3 DRAM with through-Si-via is presented which overcomes the limits of conventional modules. A master-slave architecture is proposed which decreases the standby and active power by 50 and 25%, respectively. It also increases the I/O speed to <formula formulatype="inline"><tex Notation="TeX">${&gt;}\,$</tex> </formula>1600 Mb/s for 4 rank/module and 2 module/channel case since the master isolates all chip I/O loadings from the channel. Statistical analysis shows that the proposed TSV check and repair scheme can increase the assembly yield up to 98%. By providing extra VDD/VSS edge pads, power noise is reduced to <formula formulatype="inline"><tex Notation="TeX">${≪}\,$</tex></formula>100 mV even if all 4 ranks are refreshed every clock cycle consecutively. </para>
Recombinant Adeno-Associated Viral Vectors Mediate Long-Term Transgene Expression in Muscle (1997)

Gene transfer to muscle holds overt promise for the treatment of inherited myopathies, lysosomal storage disorders, and serum protein deficiencies. In addition, muscle could provide a reservoir for delivery of therapeutic molecules like blood clotting factors, erythropoietin, or insulin. To date, successful gene transfer to muscle has been limited by the inefficiency of the vector delivery systems and the transient nature of gene expression. In this paper, we show that a vector based on recombinant adeno-associated virus (rAAV) can efficiently transduce adult mouse skeletal muscle. Transduced myofibers escape immune elimination and transgene expression is robust beyond 5 months. Importantly, input vector DNA appears to undergo conversion from single-stranded genomes to high-molecular-weight concatameric forms. These data suggest that rAAV might have a significant advantage over many other viral and nonviral gene delivery methods, and holds significant promise as a vector for gene transfer to mature muscle.
The DNA-dependent protein kinase, DNA-PK: 10 years and no ends in sight (1996)

The phenomenon of DNA-dependent protein phosphorylation was first described little over 10 years ago. Since then a DNA-dependent protein kinase, DNA-PK, has been purified from human cells and many of its biochemical properties have been characterized. DNA-PK is composed of a large catalytic subunit, DNA-PKcs, and a DNA-targeting protein, Ku. When assembled on a suitable DNA molecule, the DNA-PK holoenzyme acts as a serine/threonine protein kinase that in vitro phosphorylates many DNA binding and non-binding proteins and transcription factors. Recent genetic studies point strongly to functions in DNA double-strand break repair and V(D)J recombination. In addition, biochemical studies suggest a role in the regulation of transcription. Here we discuss, from a historical perspective, the events leading up to our current understanding of the function of DNA-PK, including recent results from our own studies suggesting the involvement of DNA-PK in apoptosis and in viral infection of human cells.
Molecular and phenotypic heterogeneity in mitochondrial trifunctional protein deficiency due to ?-subunit mutations (2003)

The mitochondrial trifunctional protein (TFP) is a multienzyme complex of the fatty acid beta-oxidation cycle. It is composed of four alpha-subunits (HADHA) harboring long-chain enoyl-CoA hydratase and long-chain L-3-hydroxyacyl-CoA dehydrogenase (LCHAD) and four beta-subunits (HADHB) harboring long-chain 3-ketoacyl-CoA thiolase (LKAT). Mutations in either subunit can result in TFP deficiency with reduced activity of all three TFP enzymes. We characterize 15 patients from 13 families with beta-subunit mutations by clinical, biochemical, and molecular features. Three clinical phenotypes are apparent: a severe neonatal presentation with cardiomyopathy, Reye-like symptoms, and early death (n=4); a hepatic form with recurrent hypoketotic hypoglycemia (n=2); and a milder later-onset neuromyopathic phenotype with episodic myoglobinuria (n=9). Maternal HELLP syndrome occurred in two mothers independently of the fetal phenotype. Mutational analysis revealed 16 different mutations, the majority being missense mutations (n=12). The predominance of missense mutations and the milder myopathic phenotype are consistent. Based upon homology to yeast thiolase that has been characterized structurally, the mutation localization within the protein correlates with the clinical phenotype. Outer loop mutations that are expected to alter protein stability less were only present in milder forms. The degree of reduction in thiolase antigen also correlated with the severity of clinical presentation. Although TFP deficiency is highly heterogeneous, there is genotype-phenotype correlation.
Hierarchies and relative operators in the OLAP environment (2000)

In the last few years, numerous proposals for modelling and querying Multidimensional Databases (MDDB) are proposed. A rigorous classification of the different types of hierarchies is still an open problem. In this paper we propose and discuss some different types of hierarchies within a single dimension of a cube. These hierarchies divide in different levels of aggregation a single dimension. Depending on them, we discuss the characterization of some OLAP operators that refer to hierarchies in order to maintain the data cube consistency. Moreover, we propose a set of operators for changing the hierarchy structure. The issues discussed provide modelling flexibility during the scheme design phase and correct data analysis.
Generating Cancelable Fingerprint Templates (2007)

Biometrics-based authentication systems offer obvious usability advantages over traditional password and token-based authentication schemes. However, biometrics raises several privacy concerns. A biometric is permanently associated with a user and cannot be changed. Hence, if a biometric identifier is compromised, it is lost forever and possibly for every application where the biometric is used. Moreover, if the same biometric is used in multiple applications, a user can potentially be tracked from one application to the next by cross-matching biometric databases. In this paper, we demonstrate several methods to generate multiple cancelable identifiers from fingerprint images to overcome these problems. In essence, a user can be given as many biometric identifiers as needed by issuing a new transformation "key." The identifiers can be cancelled and replaced when compromised. We empirically compare the performance of several algorithms such as Cartesian, polar, and surface folding transformations of the minutiae positions. It is demonstrated through multiple experiments that we can achieve revocability and prevent cross-matching of biometric databases. It is also shown that the transforms are noninvertible by demonstrating that it is computationally as hard to recover the original biometric identifier from a transformed version as by randomly guessing. Based on these empirical results and a theoretical analysis we conclude that feature-level cancelable biometric construction is practicable in large biometric deployments.
The utility of troponin measurement to detect myocardial infarction: review of the current findings (2010)

Myocardial infarction (MI) is defined by the presence of myocardial necrosis in combination with clinical evidence of myocardial ischemia. Cardiac troponins are regulatory proteins within the myocardium that are released into the circulation when damage to the myocyte has occurred. Therefore, serum troponin is an exquisitely sensitive marker of myocardial injury and is necessary for establishing the diagnosis of MI. High-sensitivity troponin assays are improving the diagnostic accuracy and rapid detection of myocardial infarction. The early identification of MI is vital for the institution of anti-thrombotic therapy to limit myocardial damage and preserve cardiac function. Troponin has both diagnostic and prognostic significance in the setting of acute coronary syndrome (ACS). Increased troponin levels in the absence of ACS should prompt an evaluation for an alternative, non-thrombotic mechanism of troponin elevation and direct management at the underlying cause. This review describes the role of troponin in the evaluation of patients with suspected myocardial infarction.
Integrated and interactive position tracking and imaging of interventional tools and internal devices using small fiducial receiver coils (1998)

A method is described of tracking the position of a rigid device within an MR scanner and imaging with the image slice position determined by the current position of the device, such that the manipulation of the device can be achieved in a robust, interactive manner. The device can be either external or internal to the patient. The position tracking is performed by means of two or three small MR receiver coils attached to individual receiver channels. Each coil contains a small sample that acts as a fiducial (MR-visible marker point). The imaging is performed by any suitable receiver coil attached to a further receive channel. This method has a large number of applications, both in the field of MR-guided procedures and in MR imaging using endocavitary coils. In particular, two devices are described, the first being a device for MR-guided biopsies and the second being one for dynamic endorectal imaging of the anal sphincter.
Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks (2007)

The stx1 and stx2 genes used in this phylogenetic study show sequence conservation with no significant divergence with respect to place or time. These data could indicate that Shiga toxins are experiencing purifying selection.
An Ocean Bottom Seismic Data Recorder (2003)

Abstract An ocean bottom seismic data recorder is a broadband digital instrument of large dynamic range, and it can be used for recording the seismic data on the ocean bottom or in remote land areas. It is a compact, light‐weight and easy operation, adopting two buffer memories to control the PC/104 embedded computer, so that it can record the seismic data continuously over long time at a low‐power dissipation. The key component of the recorder is the PC/104 embedded computer, which ensures the reliable operation of the recorder. It uses a hard disk to store data. The 2GB hard disk can hold 1.9998GB seismic data. The time length of recording the seismic data is decided by the sampling rate.
Aromatic and Pseudoaromatic Non-benzenoid Systems. III. The Synthesis of Some Ten π-Electron Systems1-3 (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAromatic and Pseudoaromatic Non-benzenoid Systems. III. The Synthesis of Some Ten π-Electron Systems1-3Norman L. Allinger and Gilbert A. YoungdaleCite this: J. Am. Chem. Soc. 1962, 84, 6, 1020–1026Publication Date (Print):March 1, 1962Publication History Published online1 May 2002Published inissue 1 March 1962https://pubs.acs.org/doi/10.1021/ja00865a030https://doi.org/10.1021/ja00865a030research-articleACS PublicationsRequest reuse permissionsArticle Views608Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts