gebhart/patrepeval huggingface dataset:
Antibodies to vascular endothelial growth factor enhance the efficacy of cancer immunotherapy by improving endogenous dendritic cell function. (1999)

Inadequate function of dendritic cells (DCs) in tumor-bearing hosts is one mechanism of tumor escape from immune system control and may compromise the efficacy of cancer immunotherapy. Vascular endothelial growth factor (VEGF), produced by most tumors, not only plays an important role in tumor angiogenesis but also can inhibit the maturation of DCs from hematopoietic progenitors. Here, we investigate a novel combination of antiangiogenic and immunotherapy based on this dual role of VEGF. Two s.c. mouse tumor models were used: D459 cells, expressing mutant human p53; and MethA sarcoma with point mutations in the endogenous murine p53 gene. Therapy with anti-mouse VEGF antibody (10 microg i.p. twice a week over 4 weeks) was initiated when tumors became palpable. Treatment of established tumors with anti-VEGF antibody alone did not affect the rate of tumor growth. However, anti-VEGF antibody significantly improved the number and function of lymph node and spleen DCs in these tumor-bearing animals. To investigate the possible effects of this antibody on the immunotherapy of established tumors, tumor-bearing mice were immunized with DCs pulsed with the corresponding mutation-specific p53 peptides, together with injections of anti-VEGF antibody. Therapy with peptide-pulsed DCs alone resulted in considerable slowing of tumor growth but only during the period of treatment, and tumor growth resumed after the end of the therapy. Combined treatment with peptide-pulsed DCs and anti-VEGF antibody resulted in a prolonged and much more pronounced antitumor effect. This effect was associated with the induction of significant anti-p53 CTL responses only in this group of mice. These data suggest that inhibition of VEGF may be a valuable adjuvant in the immunotherapy of cancer.
Teletext Field Trials in the United Kingdom (1979)

Following the introduction of an experimental Telextext service by Independent Television in June 1975, the Independent Broadcasting Authority initiated an extensive programme of field survey work.
[Observation on therapeutic effect of point-through-point acupunture on strephenopodia after apoplexy]. (2006)

The point-through-point acupuncture has obvious therapeutic effect on foot functional activities of various directions in the patient of strephenopodia after apoplexy.
Potent topoisomerase I inhibition by novel silatecans eliminates glioma proliferation in vitro and in vivo. (1999)

Although topoisomerase inhibitors, such as camptothecin and topotecan, have been widely used in the treatment of nonglial tumors, their application for gliomas has been limited by poor efficacy relative to toxicity that may in part reflect limited bioavailability and blood stability of these agents. However, the potential promise of this class of agents has fostered efforts to develop new, more potent, and less toxic inhibitors that may be clinically relevant. Using a cascade radical annulation route to the camptothecin family, we developed a series of novel camptothecin analogues, 7-silylcamptothecins ("silatecans"), that exhibited potent inhibition of topoisomerase I, dramatically improved blood stability, and sufficient lipophilicity to favor blood-brain barrier transit. We explored the efficacy of a series of these agents against a panel of five high-grade glioma cell lines to identify a promising compound for further preclinical testing. One of the most active agents in our systems (DB67) inhibited tumor growth in vitro with an ED50 ranging between 2 and 40 ng/ml, at least 10-fold more potent than the effects observed with topotecan, and at least comparable with those of SN-38, the active metabolite of CPT-11. Because DB67 also exhibited the highest human blood stability of any of the agents examined, this agent was then selected for in vivo studies. A dose-escalation study of this agent in a nude mouse U87 glioma model system demonstrated a concentration-dependent effect, with tumor growth inhibition at day 28 postimplantation (the day control animals began to require sacrifice because of large tumor size) of 61 +/- 7% and 73 +/- 3% after administration of DB67 doses of 3 and 10 mg/kg/day, respectively, for 5 days beginning on postimplantation day 7. Animals that continued treatment with 10 mg/kg/day in three additional 21-day cycles all remained progression free after >90 days of follow-up but later developed enlarging tumors after treatment was stopped. However, a slightly higher dose (30 mg/kg/day) induced complete tumor regression after only two cycles in all study animals and was effective even if treatment was delayed until large, bulky tumors had developed. Application of the 30 mg/kg/day dose to treat established intracranial glioma xenografts led to long-term (>90 day) survival in six of six animals, whereas all controls died of progressive disease (P < 0.00001). No apparent toxicity was encountered in any of the treated animals. In summary, the present studies indicate that silatecans may hold significant promise for the treatment of high-grade gliomas and provide a rationale for proceeding with further preclinical evaluation of their efficacy and safety versus commercially available camptothecin derivatives.
Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis (2015)

Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro-B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated.
Impact of V2G on real-time adaptive Volt/VAr optimization of distribution networks (2013)

Deployment of Smartgrid downstream features such as Smart Metering, pervasive control and Distributed Management Systems has brought great opportunities for distribution network planners to optimize the network in more precise methods. Moreover, the advent of Electric Vehicles (EVs) has brought more opportunities for grid optimization. Recent studies stipulate that EVs are able to inject reactive power into the grid by changing their inverter's operating mode. This paper primarily discusses a real-time adaptive Volt/VAr Optimization (VVO) engine, designed to minimize system apparent power losses, optimize voltage profiles, and reduce the operating costs of Switched Capacitor Banks of the grid. The paper goes on to study the impact of EVs on the distribution network VVO, taking into account different EV charging and penetration levels and checks the validity of the proposed algorithm by employing revised IEEE-37 Node Test Feeder in presence of various load types as a case study.
Effects of incompatible boundary information in EIT on the convergence behavior of an iterative algorithm (2002)

In electrical impedance tomography, currents are applied to the body through electrodes that are attached to the surface and the corresponding surface voltages are measured. Based on these boundary measurements, the internal admittivity distribution of the body can be reconstructed. In order to improve the image quality it is necessary and useful to apply physiologically meaningful prior information into the image reconstruction. Such prior information usually can be obtained from other sources. For example, information on the object's boundary shape and internal structure can be obtained from computed tomography and magnetic resonance imaging scan. However, this type of prior information may change from time to time and from person to person. As these changes are limited anatomically and physiologically, the prior information including the possible changes can be presented in a number of variational forms. The aim of this paper is to find which form of prior information is more compatible for a specific imaged object at the time of imaging. This paper proposes a new method for selecting the most appropriate form of prior information, through the procedure of iterative image reconstruction by using the information obtained from boundary measurements. The method is based on the principle that incompatible prior information causes errors which are able to affect the image reconstruction's convergence behavior. In this method, according to the various forms of prior information available, several image reconstruction configurations are designed. Then, through monitoring the convergence behavior in an iterative image reconstruction, the configuration with compatible prior information can be found among those different configurations. As an example, the prior information regarding the imaged object's boundary shape and internal structure was studied by computer simulation. Results were shown and discussed.
Migration of plastic packaging light stabilizers into food simulators : their reactivity in unsaturated fatty esters (1994)

Plastic packaging is usually stabilized against the effects of light by hindered amine light stabilizers (HALS : piperidine derivatives) and by UV absorbers (UVA), The amine stabilizers can react with oxygenated groups to form stable aminoxy radicals (NO°) that can be specifically detected by Electron Spin Resonance.
Foam and emulsion effects on gas driven oil recovery (1988)

The aim of this research was to investigate the gas mobility reducing effects that a gas driven surfactant slug has on enhanced oil recovery (EOR). Three chemically similar surfactants whose properties graded from foaming agent to emulsifying agent were used to study the effects that foam and emulsion formation have on enhanced oil recovery in an unconsolidated Ottawa sand model at room temperature. Both the foam lamellae and the emulsion droplets act to reduce the mobility of the injected gas in the swept zone, thus increasing the vertical sweep efficiency. Shell's Enordet series of alcohol ethoxylate surfactants were used in the study at three different concentrations of, 0.01%, 0.03% and 0.100% (wt.). The experimental procedure consisted of displacing oil from a porous medium at residual water saturation by injecting carbon dioxide, followed first by the injection of a 0.20 pore volume slug of surfactant solution, then by carbon dioxide gas at low pressure. Measurements were made of the cumulative produced gas and liquids. Performance differences between different surfactants are small but consistent. Combining the foam and emulsion mechanisms seems to lead to more efficient oil recovery than either mechanism alone. 33 refs., 14 figs., 3 tabs.
METALLO-ORGANIC COMPOUNDS CONTAINING METAL–NITROGEN BONDS: PART III. DIALKYLAMINO COMPOUNDS OF TANTALUM (1962)

Reactions involving TaCl 5 and LiX (where X = NMe 2 , NEt 2 , NPr 2 n , NBu 2 n , NMeBu n , and NC 5 H 10 ) were shown to produce substantially the pentaderivatives TaX 5 . The thermal decomposition of TaX 5 (X = NMe 2 , NEt 2 , NPr 2 n , NBu 2 n , and NMeBu n ) was studied and only Ta(NMe 2 ) 5 could be sublimed unchanged. In other cases the main product of decomposition was a quinquevalent compound RN=Ta(NR 2 ) 3 and the secondary amine R 2 NH. Some olefin and quadrivalent Ta(NR 2 ) 4 were also detected and it is believed that decomposition involves the following sequence:[Formula: see text]This is in contrast to the behavior of niobium compounds, which appears to be:[Formula: see text]
Bulk Raman Analysis of Pharmaceutical Tablets (2006)

We compare and contrast two Raman collection geometries, backscattering and transmission, to identify their potential for monitoring the bulk chemical composition of turbid media. The experiments performed on pharmaceutical tablets confirm the expected strong bias of the backscattering Raman collection towards surface layers of the probed sample. However, this bias is largely absent with the transmission geometry, exhibiting gross insensitivity to the depth of impurities within the sample. The results are supported by Monte-Carlo simulations. The applicability of transmission geometry to tablets without any thinning is possible because of long migration times of Raman photons in non-absorbing powder media. The absolute measured intensity of the Raman signal was only 12 times lower in transmission geometry compared with backscattering geometry for a standard paracetamol tablet with a thickness of 3.9 mm. This makes detection relatively straightforward, and detectable Raman signals were observed even after propagation through three paracetamol tablets. Given its properties and instrumental simplicity, the transmission method is particularly well suited to the on-line analysis of bulk content of tablets in pharmaceutical applications.
Conversion of Azides into Diazo Compounds in Water (2013)

Diazo compounds are in widespread use in synthetic organic chemistry but have untapped potential in chemical biology. We report on the design and optimization of a phosphinoester that mediates the efficient conversion of azides into diazo compounds in phosphate buffer at neutral pH and room temperature. High yields are maintained in the presence of common nucleophilic or electrophilic functional groups, and reaction progress can be monitored by colorimetry. As azido groups are easy to install and maintain in biopolymers or their ligands, this new mode of azide reactivity could have substantial utility in chemical biology.
Design and Chemical Synthesis of a Neoprotein Structural Model for the Cytoplasmic Domain of a Multisubunit Cell-Surface Receptor: Integrin .alpha.IIb.beta.3 (Platelet GPIIb-IIIa) (1994)

Integrins are a class of heterodimeric cell adhesion receptors involved in cell migration, cell anchorage, and cell-cell interactions. The cytoplasmic domains of integrins are of key importance in these activities. We have designed and chemically synthesized a 126 amino acid model protein (MP-1) containing both cytoplasmic tails of the platelet-derived integrin alpha IIb beta 3 covalently linked via a helical coiled coil. The coiled-coil tertiary structure was incorporated to mimic the membrane-spanning domain of the integrin and to act as a topological constraint fixing the two cytoplasmic tails in a parallel arrangement. This molecule, which contains two C-termini, was constructed by chemical dovetailing. The bromoacetylated and cysteinyl peptide synthons were unambiguously ligated through the formation of a thioether linkage. Ultraviolet circular dichroism (CD) spectroscopy has been performed on MP-1 and related compounds, confirming that a helical coiled coil is present within the MP-1 molecule. Significantly, the helicity apparently extends beyond the predicted amphiphilic region of MP-1. Fluorescence measurements suggest that a defined tertiary structure has formed by the association of the two cytoplasmic domains. We conclude that this is a practical design strategy for the study of the cytoplasmic domain of multisubunit cell-surface receptors.
Targeting of Porous Hybrid Silica Nanoparticles to Cancer Cells (2008)

Mesoporous silica nanoparticles functionalized by surface hyperbranching polymerization of poly(ethylene imine), PEI, were further modified by introducing both fluorescent and targeting moieties, with the aim of specifically targeting cancer cells. Owing to the high abundance of folate receptors in many cancer cells as compared to normal cells, folic acid was used as the targeting ligand. The internalization of the particles in cell lines expressing different levels of folate receptors was studied. Flow cytometry was used to quantify the mean number of nanoparticles internalized per cell. Five times more particles were internalized by cancer cells expressing folate receptors as compared to the normal cells expressing low levels of the receptor. Not only the number of nanoparticles internalized per cell, but also the fraction of cells that had internalized nanoparticles was higher. The total number of particles internalized by the cancer cells was, therefore, about an order of magnitude higher than the total number of particles internalized by normal cells, a difference high enough to be of significant biological importance. In addition, the biospecifically tagged hybrid PEI-silica particles were shown to be noncytotoxic and able to specifically target folate receptor-expressing cancer cells also under coculture conditions.
Memory-Efficient Sum–Product Decoding of LDPC Codes (2004)

Low-density parity-check (LDPC) codes perform very close to capacity for long lengths on several channels. However, the amount of memory (fixed-point numbers that need to be stored) required for implementing the message-passing algorithm increases linearly as the number of edges in the graph increases. In this letter, we propose a decoding algorithm for decoding LDPC codes that reduces the memory requirement at the decoder. The proposed decoding algorithm can be analyzed using density evolution; further, we show how to design good LDPC codes using this. Results show that this algorithm provides almost the same performance as the conventional sum-product decoding of LDPC codes.
The thickness of the human precorneal tear film: evidence from reflection spectra. (2000)

These studies did not confirm Prydal's estimate of approximately 40 microm. Nor were there prominent peaks near Danjo's value of approximately 11 microm, except in cases of probable reflex tears. Because the reflection at the aqueous-mucus boundary would be expected to be weaker than that from the epithelial surface, the 3-microm peak is unlikely to correspond to the aqueous layer (rather than the complete tear film). The proposal that the 3-microm peak corresponds to a reflection from the front of the cornea is supported by the demonstration of a peak of similar contrast from the back of the cornea. Thus, the current evidence consistently supports a value of approximately 3 microm for the thickness of the human precorneal tear film.
Genomic DNA Sequence and Transcription Factor Binding Sites of Mouse Ninjurin (2001)

The complete genomic DNA sequence of mouse ninjurin gene has been cloned and sequenced by screening a bacterial artificial chromosome (BAC) library of mouse 129/SvJ genomic DNA. The mouse ninjurin gene comprises four exons and the translatable sequences are included in the first three exons. The putative promoter region of the mouse ninjurin gene lacks the consensus "CAAT" or "TATA" sequence. Nonetheless, it has demonstrated the promoter activity in transient transfection experiment using the construct containing putative promoter sequence of mouse ninjurin and reporter gene. The nucleotide sequence of the putative promoter region shows 83% homology with the corresponding DNA sequence of human ninjurin gene that had been previously reported, and reveals a high degree of conservation between the two species. Analysis of the DNA sequence identified the putative promoters and the binding sites for a variety of transcription factors of mouse ninjurin.
Implementation of adaptive array algorithms (1986)

Some new, efficient, and numerically stable algorithms for the recursive solution of matrix problems arising in optimal beam-forming and direction finding are described and analyzed. The matrix problems considered are systems of linear equations and spectral decomposition. While recursive solution procedures based on the matrix inversion lemma may be unstable, ours are stable. Furthermore, these algorithms are extremely fast.
CD3 zeta / eta / theta locus is colinear with and transcribed antisense to the gene encoding the transcription factor Oct-1. (1993)

CD3 zeta and eta are signal-transducing components of the TCR and are derived from alternative splicing of transcripts from a single genetic locus that also encodes CD30 theta. We have isolated two murine cDNA clones that appear to result from antisense transcription through CD3 theta-specific exon 10 and CD3 eta-specific exon 9. The sequence of these clones shows no open reading frame. Northern analysis with single stranded probes confirms the existence of a ubiquitously expressed > 12-kb polyadenylated mRNA antisense to CD3 eta. A "genomic walk," which extended 32 kb distal to murine CD3 eta exon 9, provided genomic DNA containing a more 5' portion of the antisense transcript. This probe identified two murine thymic cDNA with 91% sequence homology to the human transcription factor Oct-1. Five exons of murine Oct-1 map in an antisense orientation to the CD3 zeta/eta/theta locus on the cloned genomic sequences. The murine Oct-1 cDNA and exon 9 of CD3 eta hybridize to the same > 12-kb mRNA. Similarly, human Oct-1 and previously characterized human genomic sequences homologous to murine CD3 eta exon 9 each hybridize to the same > 15-kb human mRNA. Thus, the CD3 zeta/eta/theta and Oct-1 gene loci are partially overlapping and transcribed in opposite directions. The potential functional implications of these findings are discussed.
Differential Metastasis Inhibition by Clinically Relevant Levels of Heparins—Correlation with Selectin Inhibition, Not Antithrombotic Activity (2005)

Heparin can attenuate metastasis at clinically relevant doses, likely by inhibiting selectins. Equivalent anticoagulation alone with Fondaparinux is ineffective. Clinically approved heparins have differing abilities to inhibit selectins, likely explained by size distribution. It should be possible to size fractionate heparins and inhibit selectins at concentrations that do not have a large effect on coagulation. Caution is also raised about the current preference for smaller heparins. Despite equivalent anticoagulation, hitherto unsuspected benefits of selectin inhibition in various clinical circumstances may be unwittingly discarded.
Immunization with a Synthetic Multiepitope Antigen Induces Humoral and Cellular Immune Responses to Hepatitis C Virus in Mice (2007)

The immunogenicity of a synthetic multiepitope PCX3 antigen, which contains triple tandem repeats of five conserved epitopes from hepatitis C virus (HCV) polyprotein, was studied in BALB/c mice given three intraperitoneal injections of antigen with Freund's adjuvant. Both a strong antibody response and specific cytotoxic T lymphocytes were induced. The specific anti-PCX3 IgG was able to bind HCV particles from hepatitis C patient sera by incubation overnight. In particular, in transgenic mice with chimeric human livers, anti-PCX3 antibody was able to lower the viral load in two of five mice and to eliminate HCV infection in three of five mice by 2 wk after inoculation with HCV-positive serum from patients. These results indicated that the synthetic multiepitope PCX3 antigen elicits a potent humoral and cellular immune response against HCV.