gebhart/patrepeval huggingface dataset:
Hollow Chitosan/Poly(acrylic acid) Nanospheres as Drug Carriers (2007)

The preparation, in-vitro release, in-vitro cytotoxicity, and in-vivo drug delivery of doxorubicin (DOX)-loaded chitosan (CS)-poly(acrylic acid) (PAA) hollow nanospheres were investigated. The loading was done by dissolving a certain amount of DOX in non-cross-linked CS-PAA nanospheres aqueous solution followed by cross-linking chitosan with glutaraldehyde. The drug-loading content was up to 4.3% and the size of drug-loaded hollow nanospheres, determined by dynamic light scattering, was 118 nm. The nanospheres showed a continuous release of the entrapped DOX up to 10 days in vitro and showed comparable in-vitro cytotoxicity against HepG2 cells compared to the free DOX. In-vivo DOX delivery of DOX-loaded CS-PAA nanospheres showed that DOX concentration in blood can be maintained for a longer period than free DOX solution, and the DOX concentration in mice liver can be maintained constantly at relatively high level. The interesting feature of DOX-loaded CS-PAA hollow nanopspheres is that the loaded DOX can be delivered into the mice brain. The confocal laser scanning microscopy analysis reveals that fluorescein isothiocyanate (FITC)-labeled CS-PAA can deposit in different organs including liver, spleen, and brain.
The Use of Conventional and New Music Media: Implications for Future Technologies. (2001)

A number of new technologies such as the MP3 music compression format and the Napster file sharing network are creating new opportunities for consumers’ activities with music media. In this paper we explore current practice with both conventional and new forms of media in order to better understand what the future may hold for music consumers. We interviewed 36 music enthusiasts: 12 teenaged and 12 adult conventional music users, and 12 early adopters of MP3 technologies. The results show that rather than new music technologies replacing the old, they supplement current formats by allowing users to exploit a different set of affordances. We discuss these uses drawing implications for new music devices, services and software.
The role of transglutaminase in the rat subtotal nephrectomy model of renal fibrosis. (1997)

Tissue transglutaminase is a calcium-dependent enzyme that catalyzes the cross-linking of polypeptide chains, including those of extracellular matrix (ECM) proteins, through the formation of epsilon-(gamma-glutamyl) lysine bonds. This crosslinking leads to the formation of protein polymers that are highly resistant to degradation. As a consequence, the enzyme has been implicated in the deposition of ECM protein in fibrotic diseases such as pulmonary fibrosis and atherosclerosis. In this study, we have investigated the involvement of tissue transglutaminase in the development of kidney fibrosis in adult male Wistar rats submitted to subtotal nephrectomy (SNx). Groups of six rats were killed on days 7, 30, 90, and 120 after SNx. As previously described, these rats developed progressive glomerulosclerosis and tubulo-interstitial fibrosis. The tissue level of epsilon-(gamma-glutamyl) lysine cross-link (as determined by exhaustive proteolytic digestion followed by cation exchange chromatography) increased from 3.47+/- 0.94 (mean+/-SEM) in controls to 13.24+/-1.43 nmol/g protein 90 d after SNx, P </= 0.01. Levels of epsilon-(gamma-glutamyl) lysine cross-link correlated well with the renal fibrosis score throughout the 120 observation days (r = 0.78, P </= 0.01). Tissue homogenates showed no significant change in overall transglutaminase activity (14C putrescine incorporation assay) unless adjusted for the loss of viable tubule cells, when an increase from 5.77+/-0.35 to 13.93+/-4.21 U/mg DNA in cytosolic tissue transglutaminase activity was seen. This increase was supported by Western blot analysis, showing a parallel increase in renal tissue transglutaminase content. Immunohistochemistry demonstrated that this large increase in epsilon-(gamma-glutamyl) lysine cross-link and tissue transglutaminase took place predominantly in the cytoplasm of tubular cells, while immunofluorescence also showed low levels of the epsilon-(gamma-glutamyl) lysine cross-link in the extracellular renal interstitial space. The number of cells showing increases in tissue transglutaminase and its cross-link product, epsilon-(gamma-glutamyl) lysine appeared greater than those showing signs of typical apoptosis as determined by in situ end-labeling. This observed association between tissue transglutaminase, epsilon-(gamma-glutamyl) lysine cross-link, and renal tubulointerstitial scarring in rats submitted to SNx suggests that tissue transglutaminase may play an important role in the development of experimental renal fibrosis and the associated loss of tubule integrity.
Fault-tolerant dense multiwavelength add–drop filter with a two-dimensional digital micromirror device (1998)

A two-dimensional digital micromirror device- (DMD-) based add-drop filter is introduced for dense wavelength-division multiplexed applications. Features of the filter include polarization-insensitive operation, low interchannel cross talk parallel processing, and a fault-tolerant design. Experiments include evaluation of a parallel-beam-fed interchannel cross-talk with a 25-beam mask and a three-color (red, green, blue) filter operational test that indicates output-port average switching optical signal-to-noise and multiwavelength cross-talk ratios of 24 dB. The DMD-based average optical loss for the filter is -2.85 dB for visible-light free-space operation, whereas a -8.88-dB optical loss is measured for a fiber-coupled filter operating at 1319 nm.
Massive Transfusion (2012)

The design and implementation of massive transfusion protocols with ratio-based transfusion of blood and blood products are important and active areas of investigation. A significant yet controversial body of literature exists to support the use of hemostatic resuscitation in massive transfusion and new data to support the use of adjuncts, such as recombinant factor VIIa and tranexamic acid. We review the developments in massive transfusion research during the past 5 years, including protocol implementation, hemostatic resuscitation, the use of tranexamic acid, and goal-directed therapy for coagulopathy. Furthermore, we provide a level of evidence analysis of the data surrounding the use of component therapy and recombinant factor VIIa in massive transfusion, summary recommendations for the various agents of resuscitation, and new methods of goal-directed therapy.
Thermodynamic limits to the efficiency of solar energy conversion by quantum devices (1981)

The second law of thermodynamics imposes a strict limitation to the energy converted from direct solar radiation to useful work by a quantum device. This limitation requires that the amount of energy converted to useful work (energy in any form other than heat) can be no greater than the change in free energy of the radiation fields. Futhermore, in any real energy conversion device, not all of this available free energy in the radiation field can be converted to work because of basic limitations inherent in the device itself. A thermodynamic analysis of solar energy conversion by a completely general prototypical quantum device is presented. This device is completely described by two parameters, its operating temperature T sub R and the energy threshold of its absorption spectrum. An expression for the maximum thermodynamic efficiency of a quantum solar converter was derived in terms of these two parameters and the incident radiation spectrum. Efficiency curves for assumed solar spectral irradiance corresponding to air mass zero and air mass 1.5 are presented.
1,3-Dipolar cycloadditions of nitrones derived from the reaction of acetylenes with hydroxylamines (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1,3-Dipolar cycloadditions of nitrones derived from the reaction of acetylenes with hydroxylaminesAlbert Padwa and George S. K. WongCite this: J. Org. Chem. 1986, 51, 16, 3125–3133Publication Date (Print):August 1, 1986Publication History Published online1 May 2002Published inissue 1 August 1986https://doi.org/10.1021/jo00366a009Request reuse permissionsArticle Views1152Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alertsclose Get e-Alerts
Role of a proteolysis-inducing factor (PIF) in cachexia induced by a human melanoma (G361) (1999)

Human melanoma, G361, which induces cachexia in nude mice, has been shown to produce a proteolysis-inducing factor (PIF) of Mr 24000, which is immunologically identical to that isolated from a cachexia-inducing murine tumour (MAC16). Biosynthetic labelling of G361 cells using a combination of [35S]sulphate and [6-3H]glucosamine gave a single component of Mr 24000 after affinity chromatography employing a murine monoclonal antibody. The material contained both radiolabels and, after digestion with peptide N-glycosidase F, two fragments were produced of Mr 14000 and 10000 also containing both radiolabels. Digestion with O-glycosidase produced three fragments of Mr 14000, 6000 and 4000, the first two of which contained both radiolabels, while the third only contained 3H. This digestion pattern is the same as that previously observed with PIF from the MAC16 tumour and is commensurate with one N-linked sulphated oligosaccharide chain of Mr 10000, one O-linked sulphated oligosaccharide chain of Mr 6000 and a central polypeptide chain of Mr 4000 with some residual carbohydrate. When PIF from G361 cells was administered to female NMRI mice (20 g) a pronounced depression of body weight (1.36+/-0.36 g; P < 0.0001 from control) was observed over a 24 h period without a decrease in either food or water consumption. Body composition analysis showed a significant decrease in the non-fat carcass mass without a change in carcass fat or body water. This result suggests that depletion of lean body mass in mice bearing G361 melanoma arises from the production of PIF.
cMYB is involved in the regulation of fetal hemoglobin production in adults (2006)

A quantitative trait locus (QTL) controlling HbF levels has previously been mapped to chromosome 6q23 in an Asian-Indian kindred with beta thalassemia and heterocellular hereditary persistence of fetal hemoglobin (HPFH). Five protein-coding genes, ALDH8A1, HBS1L, cMYB, AHI1, and PDE7B reside in this 1.5-megabase (Mb) candidate interval of 6q23. To direct sequencing efforts we compared the expression profiles of these 5 genes between 12 individuals with elevated and 14 individuals with normal HbF levels during adult erythropoiesis by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Two genes, cMYB and HBS1L, demonstrated simultaneous transcriptional down-regulation in individuals with elevated HbF levels. Transfection of K562 cells encoding human cDNA of cMYB and HBS1L genes showed that, although overexpression of ectopic cMYB inhibited gamma-globin gene expression, overexpression of HBS1L had no effect. Low levels of cMYB were associated with low cell expansions, accelerated erythroid maturation, and higher number of macrophages in erythroid cell culture. These observations suggest that differences in the intrinsic levels of cMYB may account for some of the variation in adult HbF levels. The possible mechanism of cMYB influencing gamma- to beta-globin switching is discussed.
Characterization of the COQ5 Gene from Saccharomyces cerevisiae EVIDENCE FOR A C-METHYLTRANSFERASE IN UBIQUINONE BIOSYNTHESIS (1997)

Ubiquinone (coenzyme Q or Q) is a lipophilic metabolite that functions in the electron transport chain in the plasma membrane of prokaryotes and in the inner mitochondrial membrane of eukaryotes. Q-deficient mutants of Saccharomyces cerevisiae fall into eight complementation groups (coq1-coq8). Yeast mutants from the coq5 complementation group lack Q and as a result are respiration-defective and fail to grow on nonfermentable carbon sources. A nuclear gene, designated COQ5 was isolated from a yeast genomic library based on its ability to restore growth of a representative coq5 mutant on media containing glycerol as the sole carbon source. The DNA segment responsible for the complementation contained an open reading frame (GenBankTM accession number Z49210Z49210) with 44% sequence identity over 262 amino acids to UbiE, which is required for a C-methyltransferase step in the Q and menaquinone biosynthetic pathways in Escherichia coli. Both the ubiE and COQ5 coding sequences contain sequence motifs common to a wide variety of S-adenosyl-L-methionine-dependent methyltransferases. A gene fusion expressing a biotinylated form of Coq5p retains function, as assayed by the complementation of the coq5 mutant. This Coq5-biotinylated fusion protein is located in mitochondria. The synthesis of two farnesylated analogs of intermediates in the ubiquinone biosynthetic pathway is reported. These reagents have been used to develop in vitro C-methylation assays with isolated yeast mitochondria. These studies show that Coq5p is required for the C-methyltransferase step that converts 2-methoxy-6-polyprenyl-1, 4-benzoquinone to 2-methoxy-5-methyl-6-polyprenyl-1,4-benzoquinone.
The Effect of Ultrasonic Vibration on Properties of Weld Metal (2013)

Weld metal mechanical properties and weldability of materials are closely related to the microstructure of the weld metal. A significant amount of research has been studied to improve microstructure of weldments such as weld pool stirring by using magnetic arc oscillation and arc pulsation. In this work, the effect of ultrasonic vibration was used to modify weld metal solidification to improve microstructure of the weld metal. Microstructure and mechanical properties of carbon steel weld metal (ER70S-G filler metal) were studied. Filler metal was melted by using Gas Tungsten Arc Welding (GTAW) in a water-cooled copper mold. Ultrasonic vibration with a frequency of 20 kHz was applied during solidification of the weld metal. Microstructure and mechanical properties of weld metal were compared with those of conventional weld metal (no ultrasonic vibration assistance). Scanning Electron Microscopy (SEM) was also used to determine microstructure and phases at high magnification. The results showed that ultrasonic vibration applied during solidification promoted grain refinement in the weld metal. Mechanical properties of weld metal were improved significantly, microstructure analysis correlated well with the mechanical test results.
Performance, carcass, and palatability traits for cull cows fed high-energy concentrate diets for 0, 14, 28, 42, or 56 days. (1997)

Cull cows, thin to moderate in initial condition scores, were randomly assigned within breed to slaughter groups and fed a high-concentrate diet for 0, 14, 28, 42 or 56 d (n = 40). Carcass data were collected, and one side of each carcass was fabricated into boneless subprimals, lean trimmings, fat, and bone. Live and carcass weights, ADG, and dressing percentage increased through 28 d of feeding (P < .05), and lean firmness increased (P < .05) through 42 d of feeding. Adjusted preliminary yield grades and final yield grades increased (P < .05) with feeding, but not to levels requiring knife trimming of fat. Fat color became whiter (P < .05) but marbling was not affected (P > .05) by feeding. Weights of fat-free lean, fat, and bone and percentages of fat in soft tissues of cow carcasses increased (P < .05) by 28 d on feed. Overall steak tenderness was higher (P < .05) for cows fed 56 d than for cows fed 0 or 14 d. Warner-Bratzler shear force was not affected by feeding. Amounts of soluble collagen increased (P < .05) in the longissimus muscle between 0 and 28 d on feed, and total collagen decreased (P < .05) in the biceps femoris muscle between 0 and 42 d on feed. Continental European cow carcasses yielded more fat-free lean and less fat (P < .05) and dairy cows generally provided the most tender product across all slaughter periods. In general, sensory tenderness and yields of cow carcass components increased without requiring excessive trimming of fat by feeding cull beef and dairy cows for periods up to 56 d.
The integer transforms analogous to discrete trigonometric transforms (2000)

The integer transform (such as the Walsh transform) is the discrete transform that all the entries of the transform matrix are integer. It is much easier to implement because the real number multiplication operations can be avoided, but the performance is usually worse. On the other hand, the noninteger transform, such as the DFT and DCT, has a good performance, but real number multiplication is required. W derive the integer transforms analogous to some popular noninteger transforms. These integer transforms retain most of the performance quality of the original transform, but the implementation is much simpler. Especially, for the two-dimensional (2-D) block transform in image/video, the saving can be huge using integer operations. In 1989, Cham had derived the integer cosine transform. Here, we will derive the integer sine, Hartley, and Fourier transforms. We also introduce the general method to derive the integer transform from some noninteger transform. Besides, the integer transform derived by Cham still requires real number multiplication for the inverse transform. We modify the integer transform introduced by Cham and introduce the complete integer transform. It requires no real number multiplication operation, no matter what the forward or inverse transform. The integer transform we derive would be more efficient than the original transform. For example, for the 8-point DFT and IDFT, there are in total four real numbers and eight fixed-point multiplication operations required, but for the forward and inverse 8-point complete integer Fourier transforms, there are totally 20 fixed-point multiplication operations required. However, for the integer transform, the implementation is simpler, and many of the properties of the original transform are kept.
Apoptosis mediates and thymidine prevents erythroblast destruction in folate deficiency anemia. (1994)

Deficiency of the vitamin folic acid causes pancytopenia by decreasing the production of new blood cells. Although impaired DNA synthesis and destruction of hematopoietic cells have been implicated, the mechanism by which folate deficiency decreases blood cell production is uncertain. An in vitro model of folate-deficient erythropoiesis was developed by using proerythroblasts isolated from folate-deficient mice that were infected with Friend leukemia virus. Proerythroblasts from folate-deficient mice had one-tenth the total folate as did proerythroblasts from control mice. The folate-deficient proerythroblasts underwent apoptosis, a form of programmed cell death, after 20-32 h in culture in folate-deficient medium. At the time of apoptosis the cells had differentiated into the later erythroblast stages and some had begun hemoglobin synthesis. Addition of either folic acid or thymidine, but not deoxycytidine or inosine, to the folate-deficient medium prevented the apoptosis and permitted proliferation and differentiation of the proerythroblasts into reticulocytes. The prevention of apoptosis by thymidine indicates (i) that decreased thymidylate synthesis plays a role in erythroblast apoptosis and the anemia of folate deficiency and (ii) that DNA cleavage is likely to be a primary event in the apoptosis of folate-deficient erythroblasts. Apoptosis of erythroblasts in the late stages of differentiation leads to decreased erythrocyte production and to anemia. The increased erythropoietin produced in response to the anemia increases the number of erythroid progenitor cells in the differentiation stages preceding those in which the cells undergo apoptosis. This population shift to earlier stage erythroblasts and proerythroblasts is characteristic of bone marrows of individuals with folate deficiency anemia.
The Metabolic Clearance of Progesterone in the Pregnant Rat: Absence of a Physiological Role for the Lung1 (1989)

The metabolic clearance rate (MCR) of progesterone is among the highest for all steroid hormones studied, yet it is difficult to apportion this high MCR to specific organ contributions. The isolated lung has been shown to metabolize progesterone, and since this tissue receives the entire cardiac output, potentially it could make a major contribution to the overall MCR. This possibility was examined in the present study by measuring lung extraction of [3H]progesterone under steady-state conditions in the intact pregnant rat. Anesthetized rats (n = 6) were infused with [3H]progesterone via a femoral vein for 100 min on Day 16 of pregnancy. After the onset of steady state (40 min), four blood samples were obtained at 20-min intervals from the right ventricle and from the aorta, and the concentrations of [3H]progesterone and its metabolites were determined. Throughout the sampling period, mean arterial pressure and heart rate remained stable (two-way analysis of variance), as did the production rate (3.76 +/- 0.35 mg/day; mean +/- SEM) and the MCR (34.8 +/- 3.5 ml/min) of progesterone. Despite this high rate of clearance, there was no difference between the concentration of [3H]progesterone in arterial and right ventricular blood, indicating no net extraction of progesterone during passage through the lung. Furthermore, there was no change in the concentration of either lipid-soluble or aqueous-soluble [3H]progesterone metabolites during trans-lung passage. These observations demonstrate that the lung does not contribute to the MCR of progesterone when measured under physiological and steady-state conditions. Therefore, the relationship, MCR (ml/min) = whole-body extraction (%) x cardiac output (ml/min), is upheld for progesterone in the rat.
Modern Microbial Genetics (2002)

Preface. Preface to the First Edition. Introduction. Contributors. SECTION I: DNA METABOLISM. Prokaryotic DNA Replication (W. Firshein). DNA Repair Mechanisms and Mutagenesis (R. Yasbin). Gene Expression and Its Regulation (J. Helmann). Bacteriophage Genetics (B. Guttan & E. Kutter). Bacteriophage and Its Relatives (R. Hendrix). Single-Stranded DNA Phages (J. LeClerc). Restriction-Modification Systems (R. Blumenthal & X. Cheng). Recombination (S. Levene & K. Huffman). Molecular Applications (T. Geoghegan). SECTION II: GENETIC RESPONSE. Genetics of Quorum Sensing Circuitry in Pseudomonas aeruginosa: Implications for Control of Pathogenesis, Biofilm Formation and Antibiotic/Biocide Resistance (D. Hassett, et al.). Endospore Formation in Bacillus subtilis: An Example of Cell Differentiation by a Bacterium (C. Moran). Stress Shock (U. Streips). Genetic Tools for Dissecting Motility and Development of Myxococcus xanthus (P. Hartzell). Agrobacterium Genetics (W. Ream). Two-Component Regulation (K. Bayles & D. Fujimoto). Molecular Mechanisms of Quorum Sensing (C. Fuqua & M. Parsek). SECTION III: GENETIC EXCHANGE. Bacterial Transposons--An Increasingly Diverse Group of Elements (G. Whittle & A. Salyers). Transformation (U. Streips). Conjugation (R. Porter). The Subcellular Entities a.k.a. Plasmids (M. Perlin). Transduction in Gram-Negative Bacteria (G. Weinstock). Genetic Approaches in Bacteria with No Natural Genetic Systems (C. Haller & T. DiChristina). Index.