gebhart/patrepeval huggingface dataset:
The membrane attack complex of complement contributes to plasmin‐induced synthesis of platelet‐activating factor by endothelial cells and neutrophils (2003)

Thrombolytic agents, used to restore blood flow to ischaemic tissues, activate several enzymatic systems with pro-inflammatory effects, thus potentially contributing to the pathogenesis of ischaemia-reperfusion injury. Platelet-activating factor (PAF), a phospholipid mediator of inflammation, has been implicated in the pathogenesis of this process. We previously showed that the infusion of streptokinase (SK) induces the intravascular release of PAF in patients with acute myocardial infarction (AMI), and that cultured human endothelial cells (EC) synthesized PAF in response to SK and plasmin (PLN). In the present study, we investigated the role of the membrane attack complex (MAC) of complement in the PLN-induced synthesis of PAF. In vivo, we showed a correlation between the levels of soluble terminal complement components (sC5b-9) and the concentrations of PAF detected in blood of patients with AMI infused with SK. In vitro both EC and polymorphonuclear neutrophils (PMN), incubated in the presence of PLN and normal human serum, showed an intense staining for the MAC neoepitope, while no staining was detected when they were incubated with PLN in the presence of heat-inactivated normal human serum. Moreover, the insertion of MAC on EC and PMN plasmamembrane elicited the synthesis of PAF. In conclusion, our results elucidate the mechanisms involved in PAF production during the activation of the fibrinolytic system, showing a role for complement products in this setting. The release of PAF may increase the inflammatory response, thus limiting the beneficial effects of thrombolytic therapy. Moreover, it may have a pathogenic role in other pathological conditions, such as transplant rejection, tumoral angiogenesis, and septic shock, where fibrinolysis is activated.
A tutorial on decomposition methods for network utility maximization (2006)

A systematic understanding of the decomposability structures in network utility maximization is key to both resource allocation and functionality allocation. It helps us obtain the most appropriate distributed algorithm for a given network resource allocation problem, and quantifies the comparison across architectural alternatives of modularized network design. Decomposition theory naturally provides the mathematical language to build an analytic foundation for the design of modularized and distributed control of networks. In this tutorial paper, we first review the basics of convexity, Lagrange duality, distributed subgradient method, Jacobi and Gauss-Seidel iterations, and implication of different time scales of variable updates. Then, we introduce primal, dual, indirect, partial, and hierarchical decompositions, focusing on network utility maximization problem formulations and the meanings of primal and dual decompositions in terms of network architectures. Finally, we present recent examples on: systematic search for alternative decompositions; decoupling techniques for coupled objective functions; and decoupling techniques for coupled constraint sets that are not readily decomposable.
EGFR Signaling Promotes TGFβ-Dependent Renal Fibrosis (2011)

The mechanisms by which angiotensin II (Ang II) promotes renal fibrosis remain incompletely understood. Ang II both stimulates TGFβ signaling and activates the EGF receptor (EGFR), but the relative contribution of these pathways to renal fibrogenesis is unknown. Using a murine model with EGFR-deficient proximal tubules, we demonstrate that upstream activation of EGFR-dependent ERK signaling is critical for mediating sustained TGFβ expression in renal fibrosis. Persistent activation of the Ang II receptor stimulated ROS-dependent phosphorylation of Src, leading to sustained EGFR-dependent signaling for TGFβ expression. Either genetic or pharmacologic inhibition of EGFR significantly decreased TGFβ-mediated fibrogenesis. We conclude that TGFβ-mediated tissue fibrosis relies on a persistent feed-forward mechanism of EGFR/ERK activation through an unexpected signaling pathway, highlighting EGFR as a potential therapeutic target for modulating tissue fibrogenesis.
Pancreatic carcinoma cells express neuropilins and vascular endothelial growth factor, but not vascular endothelial growth factor receptors (2004)

The pancreatic carcinoma cell line Panc-1 and adenocarcinoma tissue specimens expressed high levels of NRP-1 and VEGF, but not VEGFRs, and exogenous VEGF significantly increased NRP-1-mediated, but not VEGFR-mediated, Panc-1 cell proliferation. These data suggested that NRP-1 may be involved in the pathogenesis of pancreatic carcinoma.
Synthesis of Oligonucleotide Analogues with Modified Backbones (1993)

The research activity in the general area of modified oligonucleotides and their dephospho analogues is summarized. The general criteria for the design of oligomers with altered backbones, namely charge, hydration and conformational freedom of the internucleosidyl linkage, are described. The flexible and semi-rigid polyamide linkages are emphasized which are best suited to adapt to the solid phase synthetic methodologies, at the same time satisfying the necessary physical requirements for optimum binding to form a duplex or triplex. A novel approach is described which takes advantage of the established peptide chemistry in the synthesis of oligonucleosides with an amino acid backbone. A discussion of the future direction for the synthesis and application of these polymers is included. 1. Introduction 2. Alternative Phosphate-Containing Linkages 2.1. Phosphorothioates 2.1.1. Phosphorodithioates 2.2. Methylphosphonates 2.3. Phosphoramidates 2.4. Phosphate Esters 2.5. The Contradiction Suggested by the Modified Phosphate Oligonucleotides: Influence of Backbone Charge and Hydration 3. Phosphate-Free Backbones 3.1. Carbonate Linkages 3.2. Carboxymethyl Linkages 3.3. Acetamidate Linkages 3.4. Carbamate Linkages 3.5. The Polyamide Linkages 3.5.1. The Semi-Rigid Polyamide Linkages 3.6. General Criteria for Altered Oligomer Design 3.6.1. Conformational Freedom 3.6.2. Backbone Charge 3.6.3. Backbone Hydration 3.7. Sulfur-Based Linkages 3.7.1. Thioether Linkages 3.7.2. Sulfonate, Sulfonamide and Sulfamate Linkages 3.7.3. Sulfide, Sulfoxide and Sulfone Linkages 3.8. Miscellaneous Linkages 3.8.1. Formacetal and Thioformacetal Linkages 3.8.2. Methylhydroxylamine Linkages 3.8.3. N-Cyanoguanidine Linkage 3.9. Alkylsilyl Linkages 4. Conclusion
Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae (1996)

The XYN2 gene encoding the main Trichoderma reesei QM 6a endo-beta-1,4-xylanase was amplified by PCR from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 699-bp open reading frame that encodes a 223-amino-acid propeptide. The XYN2 gene, located on URA3-based multicopy shuttle vectors, was successfully expressed in the yeast Saccharomyces cerevisiae under the control of the alcohol dehydrogenase II (ADH2) and phosphoglycerate kinase (PGK1) gene promoters and terminators, respectively. The 33-amino-acid leader peptide of the Xyn2 beta-xylanase was recognized and cleaved at the Kex2-like Lys-Arg residues, enabling the efficient secretion and glycosylation of the heterologous beta-xylanase. The molecular mass of the recombinant beta-xylanase was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 27 kDa. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of the URA3-based XYN2 shuttle vectors in nonselective complex medium. These autoselective S. cerevisiae strains produced 1,200 and 160 nkat of beta-xylanase activity per ml under the control of the ADH2 and PGK1 promoters in rich medium, respectively. The recombinant enzyme showed highest activity at pH 6 and 60 degrees C and retained more than 90% of its activity after 60 min at 50 degrees C.
Osteopontin Promotes Integrin Activation through Outside-In and Inside-Out Mechanisms: OPN-CD44V Interaction Enhances Survival in Gastrointestinal Cancer Cells (2007)

Osteopontin (OPN) and splice variants of CD44 (CD44(V)) have independently been identified as markers for tumor progression. In this study, we show that both OPN and CD44(V) are frequently overexpressed in human gastric cancer and that OPN-engaged CD44(V) ligation confers cells an increased survival mediated through integrin activation. First, we show that OPN treatment confers cells an increased resistance to UV-induced apoptosis. The OPN-mediated antiapoptosis is dependent on the expression of the variant exon 6 (V6)- or V7-containing CD44 as shown by overexpression of individual CD44(V) in gastric AZ521 cells that express no or very low level of endogenous CD44 and by knockdown of the constitutively expressed V6-containing CD44 isoforms in colon HT29 cells. Although OPN also interacts with RGD integrins, OPN-RGD sequence is dispensable for OPN-mediated antiapoptosis. OPN-induced antiapoptosis is mainly attributed to the engagement of CD44(V) isoforms and the relay of an inside-out signaling via Src activity, leading to robust integrin activation. Furthermore, OPN-elicited antiapoptosis was observed when cells were plated on fibronectin but not on poly-D-lysin, and preincubation of cells with anti-integrin beta(1) antibody to block integrin-extracellular matrix (ECM) interaction or ectopic expression of the dominant-negative forms of focal adhesion kinase to block ECM-derived signal abolished OPN-induced survival, suggesting that OPN-elicited antiapoptotic function is propagated from matrix transduced by integrin. Taken together, we showed that OPN-CD44(V) interaction promotes ECM-derived survival signal mediated through integrin activation, which may play an important role in the pathogenic development and progression of gastric cancer.
First Titanium‐Catalyzed anti‐1,4‐Hydrosilylation of Dienes. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Electrolytic membrane recovery of bromine from waste hydrogen bromide streams (1998)

Abstract A novel electrochemical process was developed for the recovery of bromine from waste gas‐phase hydrogen bromide streams. It uses a molten‐salt‐saturated membrane to electrolytically decompose hydrogen bromide into its molecular constituents, which are separated into a hydrogen‐enriched waste stream and a pure bromine product stream. Single‐cell studies were carried out in a configuration consisting of two cell housings (vitreous carbon), two gas‐diffusion electrodes (reticulated vitreous carbon or graphite felt), and a molten salt [(Li 0.575 K 0.133 Cs 0.292 )Br] saturated membrane (zirconia). Single‐cell results at 300°C, based on process stream concentrations ranging from 25 to 75% hydrogen bromide at 50 to 300 mL/min, demonstrated current densities exceeding 1 A/cm 2 and removals as high as 95%. Water and acetone (as a light organic contaminant) addition to the process feed, as well as exposure to thermal cycling, showed no deleterious effects on cell performance. Preliminary economics indicate this to be a viable process.
The Results of Repair of Massive Tears of the Rotator Cuff*† (2000)

The method of repair of massive rotator cuff tears that was used in this study yielded a comparatively low retear rate and good-to-excellent clinical results; however, the repair did not result in substantial reversal of muscular atrophy and fatty degeneration. Retears occurred more often in patients who had had a shorter interval between the onset of the symptoms and the operation (p < 0.05). Patients who had a retear had improvement of the shoulder compared with the preoperative state, but they had less improvement than did those who had a successful repair.
CMOS-compatible high efficiency double-etched apodized waveguide grating coupler (2013)

We present a high efficiency double-etched apodized fiber-to-waveguide grating coupler on a silicon-on-insulator substrate, which can be fabricated using deep UV photolithography. The fabricated grating coupler yields a coupling loss of -1.5 dB with 3-dB bandwidth of 54 nm at a wavelength of 1560 nm. Measurements and simulations show that the double-etched apodized grating coupler design is robust and tolerant to fabrication process variations.
The effects of tibolone on mood and libido (2002)

Published studies indicate beneficial effects of tibolone on both libido and mood, which otherwise significantly compromise physical, psychological, and social well-being. Hence, tibolone provides another option for menopausal women experiencing loss of libido as part of their symptomatology or who have persistent low libido despite adequate estrogen/progestin replacement therapy.
Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis. (2002)

99mTc-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction.
Differential effects of chemotherapeutic drugs versus the MDM‐2 antagonist nutlin‐3 on cell cycle progression and induction of apoptosis in SKW6.4 lymphoblastoid B‐cells (2007)

We have compared the cytotoxic/cytostatic responses of the SKW6.4 lymphoblastoid B-cells to the alkylating agent chlorambucil, the purine analog fludarabine, the non-genotoxic activator of the p53 pathway, Nutlin-3, used alone or in association with the death-inducing ligand recombinant TRAIL. Exposure to chlorambucil, fludarabine, and Nutlin-3 induced p53 accumulation and variably affected cell cycle progression in SKW6.4 lymphoblastoid cells. In particular, chlorambucil induced cell cycle accumulation at the G2/M checkpoint; Nutlin-3 induced early cell cycle arrest at the G1/S checkpoint, while fludarabine showed an intermediate behavior. On the other hand, recombinant TRAIL alone did not affect cell cycle progression but induced a rapid increase of apoptosis. Analysis of the gene expression profile of the p53-transcriptional targets showed distinct features between chlorambucil, Nutlin-3 and fludarabine, which likely account for their differential effect on cell cycle in SKW6.4 cells. In particular, chlorambucil upregulated the steady-state mRNA expression of SFN/14-3-3sigma, a gene involved in G2/M cell cycle arrest. Of note, all agonists upregulated TRAIL-R2 expression in SKW6.4 cells both at the mRNA and protein levels. Consistently, pretreatment with chlorambucil, fludarabine and Nutlin-3 enhanced SKW6.4 sensitivity to TRAIL-mediated apoptosis.
Targeting the anthrax receptors, TEM-8 and CMG-2, for anti-angiogenic therapy (2010)

The anthrax toxin receptors tumor endothelial marker-8 (TEM-8) and capillary morphogenesis gene-2 (CMG-2) are responsible for allowing entry of anthrax toxin into host cells. These receptors were first discovered due to their enhanced expression on endothelial cells undergoing blood vessel growth or angiogenesis in model systems. Inhibition of angiogenesis is an important strategy for current anti-cancer therapies and treatment of retinal diseases. Functional roles for TEM-8 and CMG-2 in angiogenesis have recently emerged. TEM-8 appears to regulate endothelial cell migration and tubule formation whereas a role for CMG-2 in endothelial proliferation has been documented. TEM-8 and CMG-2 bind differentially to extracellular matrix proteins including collagen I, collagen IV and laminin and these properties may be responsible for their apparent roles in regulating endothelial cell behavior during angiogenesis. TEM-8-binding moieties have also been suggested to be useful in selectively targeting anti-angiogenic and anti-tumorigenic therapies to tumor endothelium. Additionally, studies of modified forms of lethal toxin (LeTx) have demonstrated that targeted inhibition of MAPKs within tumor vessels may represent an efficacious anti-angiogenic strategy.
The trigger of the TOFUS detector (1992)

The TOFUS detector is a low-energy neutron detector based on double scattering and dedicated to cold fusion experiments. A solution has been found by means of the double scattering technique for neutron detection. The realized apparatus, the trigger, and the detector performance concerning the time resolution and the dead time are described. The authors have designed, built, and tested special logic circuits allowing a fast decision in a time less than 200 ns, and able to reduce the background signal to 0.035 trigger/s. Then a software analysis reduces this number of 0.007 events/s in the (2-3) MeV neutron energy range.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Characterization of a cytoplasmic trehalase of Escherichia coli (1996)

Escherichia coli can synthesize trehalose in response to osmotic stress and is able to utilize trehalose as a carbon source. The pathway of trehalose utilization is different at low and high osmolarity. At high osmolarity, a periplasmic trehalase (TreA) is induced that hydrolyzes trehalose in the periplasm to glucose. Glucose is then taken up by the phosphotransferase system. At low osmolarity, trehalose is taken up by a trehalose-specific enzyme II of the phosphotransferase system as trehalose-6-phosphate and then is hydrolyzed to glucose and glucose-6-phosphate. Here we report a novel cytoplasmic trehalase that hydrolyzes trehalose to glucose. treF, the gene encoding this enzyme, was cloned under ara promoter control. The enzyme (TreF) was purified from extracts of an overexpressing strain and characterized biochemically. It is specific for trehalose exhibiting a Km of 1.9 mM and a Vmax of 54 micromol of trehalose hydrolyzed per min per mg of protein. The enzyme is monomeric, exhibits a broad pH optimum at 6.0, and shows no metal dependency. TreF has a molecular weight of 63,703 (549 amino acids) and is highly homologous to TreA. The nonidentical amino acids of TreF are more polar and more acidic than those of TreA. The expression of treF as studied by the expression of a chromosomal treF-lacZ fusion is weakly induced by high osmolarity of the medium and is partially dependent on RpoS, the stationary-phase sigma factor. Mutants producing 17-fold more TreF than does the wild type were isolated.
CBI-TMI: Synthesis and Evaluation of a Key Analog of the Duocarmycins. Validation of a Direct Relationship between Chemical Solvolytic Stability and Cytotoxic Potency and Confirmation of the Structural Features Responsible for the Distinguishing Behavior of Enantiomeric Pairs of Agents (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCBI-TMI: Synthesis and Evaluation of a Key Analog of the Duocarmycins. Validation of a Direct Relationship between Chemical Solvolytic Stability and Cytotoxic Potency and Confirmation of the Structural Features Responsible for the Distinguishing Behavior of Enantiomeric Pairs of AgentsDale L. Boger and Weiya YunCite this: J. Am. Chem. Soc. 1994, 116, 18, 7996–8006Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September 1994https://doi.org/10.1021/ja00097a006RIGHTS & PERMISSIONSArticle Views442Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (4 MB) Get e-Alertsclose Get e-Alerts
Ionic responses and growth stimulation induced by nerve growth factor and epidermal growth factor in rat pheochromocytoma (PC12) cells. (1983)

Rat pheochromocytoma cells (clone PC12) respond to nerve growth factor (NGF) by the acquirement of a phenotype resembling neuronal cells. In an earlier study we showed that NGF causes an increase in Na+,K+ pump activity, as monitored by ouabain-sensitive Rb+ influx. Here we show that addition of epidermal growth factor (EGF) to PC12 cells resulted in a stimulation of Na+,K+ pump activity as well. The increase of Na+,K+ pump activity by NGF or EGF was due to increased Na+ influx. This increased Na+ influx was sensitive to amiloride, an inhibitor of Na+,H+ exchange. Furthermore, no changes in membrane potential were observed upon addition of NGF or EGF. Amiloride-sensitive Na+,H+ exchange in PC12 cells was demonstrated by H+ efflux measurements and the effects of weak acids on Na+ influx. These observations suggest that both NGF and EGF activate an amiloride-sensitive, electroneutral Na+,H+ exchange mechanism in PC12 cells. These findings were surprising in view of the opposite ultimate biological effects of NGF and EGF, e.g., growth arrest vs. growth stimulation. However, within 24 h after addition, NGF was found to stimulate growth of PC12 cells, comparable to EGF. In the presence of amiloride, this stimulated growth by NGF and EGF was abolished. In contrast, amiloride did not affect NGF-induced neurite outgrowth of PC12 cells. From these observations it is concluded that in PC12 cells: (a) NGF has an initial growth stimulating effect; (b) neurite outgrowth is independent of increased amiloride-sensitive Na+ influx; and (c) growth stimulation by NGF and EGF is associated with increased amiloride-sensitive Na+ influx.
IMP PCR primers detect single nucleotide polymorphisms for Anopheles gambiae species identification, Mopti and Savanna rDNA types, and resistance to dieldrin in Anopheles arabiensis (2006)

The simplicity and effectiveness of the method should be utilized in these and other PCR analyses to increase their specificity and simplicity. These results have the potential to be extended not only to mosquito analyses but also to parasite and human polymorphisms.
Preparation and characterization of ultrathin Pb and In films on PbTe and Te (1976)

Ultrathin 10–300-Å films of Pb and In have been prepared by evaporation in ultrahigh vacuum onto single crystal PbTe and Te substrates at low temperatures. Epitaxial growth was obtained for both metals deposited on PbTe (100) at 77 K, but not on Te (101̄0). Low energy electron diffraction, Auger electron spectroscopy, and low energy electron energy loss measurements were used to characterize substrates and films. Values of low energy electron escape depths through Pb, In, and PbTe were also obtained.
Plasminogen (1992)

Plasminogen is the zymogen form of plasmin, a broad specificity serine protease whose activity contributes to a variety of normal and pathological conditions, including intravascular thrombolysis and extracellular proteolysis. Plasminogen contains seven structural units or 'domains', each of which confer specific properties on the molecule. The kringle domains possess fibrin-binding functions and, together with the N-terminal peptide, regulate the ability of plasminogen to adopt at least three dissimilar conformations. These conformational forms influence the rate of formation, following activation by plasminogen activators, of the plasmin active site within its C-terminal serine protease domain. Structural and functional analogies are postulated between these plasminogen structures and the conformations of other proteins related by sequence homology.