gebhart/patrepeval huggingface dataset:
Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae (1994)

Potocytosis is an endocytic pathway that utilizes glycosylphosphatidylinositol-anchored membrane proteins and caveolae to concentrate and internalize small molecules. We now report that activators of protein kinase C are potent inhibitors of potocytosis. Activators such as phorbol-12-myristate-13-acetate (PMA) inhibit the internalization of receptors for 5-methyltetrahydrofolate but allow the internal receptor pool to return to the cell surface. PMA does not affect the clustering of the folate receptor but instead markedly reduces the number of caveolae. Exposure to PMA totally blocks the intracellular accumulation of 5-methyltetrahydrofolate without affecting receptor-independent uptake or the formation of polyglutamylated species of 5-methyltetrahydrofolate in the cytoplasm. These data suggest that PMA inhibits uptake by inactivating caveolae internalization.
Growth factor-inducible alternative splicing of nontransmembrane phosphotyrosine phosphatase PTP-1B pre-mRNA. (1993)

PTP-1B is a major nontransmembrane phosphotyrosine phosphatase in human cell lines and tissues, but its physiological function(s) and mechanism(s) of regulation are largely unknown. We have found that in human diploid fibroblasts a novel PTP-1B mRNA isoform is produced upon stimulation of quiescent cells with a variety of growth factors. Generation of this isoform requires protein synthesis, suggesting that the product(s) of an immediate early response gene(s) is required. RNase protection and reverse transcription polymerase chain reaction analysis demonstrate that the isoform arises as a consequence of alternative pre-mRNA splicing, leading to retention of the last intron. The novel isoform is found on polyribosomes, indicating that it is actively translated, and is variably expressed in human tissues. Sequence analysis indicates that the isoform encodes a PTP-1B protein with an altered C terminus. To our knowledge, this is the first example of growth factor-regulated alternative splicing.
Identification and disruption of the gene encoding the K+-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae (1998)

The identity of the gene encoding the mitochondrial K+-activated acetaldehyde dehydrogenase (K+-ACDH) of Saccharomyces cerevisiae has been confirmed. The gene is situated on the right arm of chromosome XV, bears the systematic name YOR374w and the deduced product shows significant homology to other members of the S. cerevisiae aldehyde dehydrogenase (ALDH) family. YOR374w has now been assigned the gene name ALD7. The N-terminal amino acid sequences of K+-ACDHs purified from several diverse strains of S. cerevisiae were determined, and found to have 81–100% identity in alignments with the product of ALD7. Haploid mutants containing a deletion of ALD7 were constructed and, in these strains, the K+-ACDH was not detectable under any growth conditions examined. The activity of the Mg2+-activated acetaldehyde dehydrogenase (Mg2+-ACDH), encoded by ALD6, remained at wild-type levels in the mutants. Growth on glucose was not affected in the mutants lacking ALD7 (in contrast to the behaviour of ald6 mutants), whereas growth on ethanol was severely impaired. This observation, together with previous work by our group, shows that both the Mg2+- and K+-ACDHs are required for growth on ethanol, whilst only the former plays a role during growth on glucose.
Update of the NCI multiinstitutional phase II trial of romidepsin, FK228, for patients with cutaneous or peripheral T-cell lymphoma (2007)

8027 Background: The histone deacetylase inhibitors (HDIs) are a class of differentiating agents undergoing clinical testing. Like other HDIs, romidepsin (FK228) modulates expression of genes involved in cell cycle regulation and markers of differentiation in cancer cell lines, leading to induction of differentiation or apoptosis. Romidepsin has demonstrated clinical activity in patients with T-cell lymphoma. Methods: Patients with CTCL (42) or PTCL (36) were enrolled in the NCI multi-institutional trial and assigned to cohorts based on extent of prior therapy and pathology. Romidepsin is administered on days 1, 8, and 15 of a 28 d cycle with a starting dose of 14mg/m 2 . Responses for patients with PTCL are measured using Cheson criteria, and CTCL using RECIST criteria. Results: Cohort one, composed of 27 patients who had previously received no more than 2 prior cytotoxic regimens of chemotherapy, has completed enrollment. Responses observed include 3 patients with CR and 7 patients with partial responses, yielding an overall response rate of 37%. Of note, responses were observed independent of stage of disease. Among 18 patients with stage IV disease, 6 patients had a complete or partial response, including 3 patients with Sézary syndrome. When including patients with greater than 2 prior cytotoxic regimens, the overall response rate was 31%. A replicate arm has been opened with the goal of confirming the response rate observed in the first cohort. Response data have not been evaluated from this arm at this time. Responses observed in 36 patients with refractory or relapsed PTCL includes 3 patients with CR and 8 patients with partial responses, comprising an overall response rate of 30%. Responses were observed independent of prior therapy, with some patients having undergone prior stem-cell transplant. Molecular endpoint analysis was performed on peripheral mononuclear cells (PBMNCs) and tumor biopsies from treated patients evaluating histone acetylation and changes in gene expression. Conclusions: Romidepsin as a single agent appears to have significant single agent activity in patients with CTCL and PTCL. Combination therapy with romidepsin may increase efficacy and should be pursued. This protocol remains open to accrual. No significant financial relationships to disclose.
68‐1: A Machine Learning Approach to Objective Image Quality Evaluation (2019)

Subjective Test Scoring is the gold standard for evaluating visual lossless image compression, but its high cost and long lead time prohibits testing more than a few images. Here, we model the display and human eye and use Machine Learning models of increased complexity, achieving a Pearson correlation of 0.95.
Human immunodeficiency virus infection of xenografted SCID‐beige mice (1995)

The application of C.B-17 SCID-Beige mice as an experimental animal system for the acceptance of human leukocyte xenografts, the establishment of functional human immune responses and infection with HIV has been assessed. Reconstitution efficiencies approaching 100% could be obtained by using 2 x 10(7) human peripheral blood lymphocytes (PBLs). Typical levels of human immunoglobulin in mouse blood reached 120 micrograms/ml within 2 weeks of reconstitution rising to a maximum in excess of 3 mg/ml by 5 weeks. Immunohistological examination of lung, spleen, lymph node and thymus tissue, derived from reconstituted mice, with human leukocyte specific monoclonal antibodies revealed the presence of human macrophages (CD68+), T cells (CD43+) and B cells (CD20+). The establishment of a functional immune system was demonstrated by the ability of reconstituted mice to respond to immunisation with KLH. Finally, reconstituted Hu-PBL-SCID-Beige mice were susceptible to infection with HIV-1 by intraperitoneal injection. These results indicate that SCID-Beige mice are a valuable tool for the generation of human/mouse chimeras and for the establishment of an in vivo HIV infection model. The results are compared with other similar model systems and are discussed in the context of animal models of HIV vaccine studies.
Thrombin Activatable Fibrinolysis Inhibitor, a Potential Regulator of Vascular Inflammation (2003)

The latent plasma carboxypeptidase thrombin-activable fibrinolysis inhibitor (TAFI) is activated by thrombin/thrombomodulin on the endothelial cell surface, and functions in dampening fibrinolysis. In this study, we examined the effect of activated TAFI (TAFIa) in modulating the proinflammatory functions of bradykinin, complement C5a, and thrombin-cleaved osteopontin. Hydrolysis of bradykinin and C5a and thrombin-cleaved osteopontin peptides by TAFIa was as efficient as that of plasmin-cleaved fibrin peptides, indicating that these are also good substrates for TAFIa. Plasma carboxypeptidase N, generally regarded as the physiological regulator of kinins, was much less efficient than TAFIa. TAFIa abrogated C5a-induced neutrophil activation in vitro. Jurkat cell adhesion to osteopontin was markedly enhanced by thrombin cleavage of osteopontin. This was abolished by TAFIa treatment due to the removal of the C-terminal Arg168 by TAFIa from the exposed SVVYGLR alpha 4 beta 1 integrin-binding site in thrombin-cleaved osteopontin. Thus, thrombin cleavage of osteopontin followed by TAFIa treatment may sequentially up- and down-modulate the pro-inflammatory properties of osteopontin. An engineered anticoagulant thrombin, E229K, was able to activate endogenous plasma TAFI in mice, and E229K thrombin infusion effectively blocked bradykinin-induced hypotension in wild-type, but not in TAFI-deficient, mice in vivo. Our data suggest that TAFIa may have a broad anti-inflammatory role, and its function is not restricted to fibrinolysis.
p-Isothiocyanatobenzyl-desferrioxamine: a new bifunctional chelate for facile radiolabeling of monoclonal antibodies with zirconium-89 for immuno-PET imaging (2009)

The novel Df-Bz-NCS BFC allows efficient and easy preparation of optimally performing (89)Zr-labeled mAbs, facilitating further exploration of (89)Zr-immuno-PET as an imaging tool.
Gene Therapy for the Treatment of Metastatic Breast Cancer by In Vivo Transduction with Breast-Targeted Retroviral Vector Expressing Antisense c-fos RNA. Vanderbilt University Medical School, Nashville, Tennessee (1996)

Scientific Abstract Although murine retroviral vectors can transduce diverse types of proliferating cells, inclusion of tissue-specific or inducible promoters within retroviral vectors should theoretically target expression to allow selective expression within tumor cells. The mouse mammary tumor virus (MMTV) long terminal repeat (LTR) is expressed at high levels in only certain tissues in vivo: breast, prostate, salivary gland. We have employed these vectors to transduce MCF-7 human breast cancer cells. Transduction of cultured cells by supernatants from cloned producer cells expressing either antisense c-fos RNA or antisense c-myc RNA (titer 9 × 105 virions/ml) produces a greater than 90% reduction in the tumor size when cells are injected into an estrogen-dependent nude mouse model (compared with control MMTV-based viral vectors). Based upon these findings, we have proposed a clinical trial to determine if infusion of antisense c-fos viral vectors will induce regression of metastatic breast cancer in the peritoneum (malignant ascites) or pleura (malignant pleuritis). The patient population will consist of women who have failed standard therapy and have extensive, biopsy or cytology-proven metastatic breast cancer involving the peritoneum or pleura which result in malignant effusions. In this protocol, patient effusions will be drained daily for 4 days and the fluid replaced with retroviral vector. These are patients who have failed standard therapy for their cancer and are expected to survive for a few months.
Evaluation of the Mtb72F Polyprotein Vaccine in a Rabbit Model of Tuberculous Meningitis (2006)

Using a rabbit model of tuberculous meningitis, we evaluated the protective efficacy of vaccination with the recombinant polyprotein Mtb72F, which is formulated in two alternative adjuvants, AS02A and AS01B, and compared this to vaccination with Mycobacterium bovis bacillus Calmette-Guérin (BCG) alone or as a BCG prime/Mtb72F-boost regimen. Vaccination with Mtb72F formulated in AS02A (Mtb72F+AS02A) or Mtb72F formulated in AS01B (Mtb72F+AS01B) was protective against central nervous system (CNS) challenge with Mycobacterium tuberculosis H37Rv to an extent comparable to that of vaccination with BCG. Similar accelerated clearances of bacilli from the cerebrospinal fluid, reduced leukocytosis, and less pathology of the brain and lungs were noted. Weight loss of infected rabbits was less extensive for Mtb72F+AS02A-vaccinated rabbits. In addition, protection against M. tuberculosis H37Rv CNS infection afforded by BCG/Mtb72F in a prime-boost strategy was similar to that by BCG alone. Interestingly, Mtb72F+AS01B induced better protection against leukocytosis and weight loss, suggesting that the polyprotein in this adjuvant may boost immunity without exacerbating inflammation in previously BCG-vaccinated individuals.
Cyclic Stretching Stimulates Synthesis of Matrix Components by Arterial Smooth Muscle Cells in Vitro (1976)

Rabbit aortic medial cells were grown on purified elastin membranes, which were then subjected to repeated elongation and relaxation or to agitation without stretching. Cells remained attached to the membranes, and cyclic stretching resulted in a two- to fourfold increase in rates of collagen, hyaluronate, and chondroitin 6-sulfate synthesis over those in agitated or stationary preparations. Synthesis of types I and III collagen was increased to the same degree. Stretching did not increase rates of chondroitin 4-sulfate or dermatan sulfate synthesis. Differences were not attributable to differences in cell number, for DNA synthetic rates were not increased by stretching. The model system devised to demonstrate these effects provides a means for relating various modes of mechanical stimulation to cell metabolism.
Biomechanics of the Spine (2005)

The field of modern biomechanics has deep historical roots from the ancient Egyptians, who documented the earliest accounts of spinal injury (2600-2200 BC), as well as from the ancient Hindus, who were noted for their treatment of spinal deformities (3500-1800 BC). Building on this foundation, the modern explosion of spinal instrumentation introduced the concept of internal fixation for spinal stabilization, further advancing the understanding of the mechanics of musculoskeletal motion. The spine is a complex mechanical structure complete with levers (vertebrae), pivots (facets and disks), passive restraints (ligaments), and actuators (muscles). Each of these elements merits special consideration and thus is addressed individually. Understanding actions and reactions, force vectors, related component vectors, and the movements and/or deformation that they cause allows the spine surgeon to apply fundamental physical principles to clinical practice. Kinematics is the application of these physical principles toward the study of the motion of rigid bodies. Knowledge of the principles and laws that are clinically relevant regarding spinal instrumentation is crucial to success. Clinical biomechanics requires the assessment of 3 key questions: 1) how do the components of the implant connect together, 2) how does the implant connect to the spine, and 3) how does the construct function biomechanically? The ability to apply basic biomechanical principles in the clinical arena provides a framework that the surgeon can use in the clinical decision-making process.
Specific Knockdown of Nanog Expression by RNA Interference in P19 Embryonal Carcinoma Stem Cells (2008)

Nanog is a newly identified member of the homeobox family of DNA binding transcription factors that functions to maintain the undifferentiated state of stem cells. However, molecular mechanisms underlying the function of Nanog remain largely unknown. To elucidate the regulatory roles of Nanog involved in maintenance of P19 embryonal carcinoma (EC) stem cells, we transfected three small interfering RNA (siRNA) duplexes targeted against different regions of the Nanog gene into P19 cells. The Nanog siRNA-100 duplexes effectively decreased the expression of Nanog up to 30.7% compared to other two Nanog siRNAs, the Nanog siRNA-400 (67.9 %) and -793 (53.0%). When examined by RT-PCR and real-time PCR, the expression of markers for pluripotency such as Fgf4, Oct3/4, Rex1, Sox1 and Yes was downregulated at 48 h after transfection with Nanog siRNA-100. Furthermore, expression of the ectodermal markers, Fgf5 and Isl1 was reduced by Nanog knockdown. By contrast, the expression of other markers for pluripotency such as Cripto, Sox2 and Zfp57 was not affected by Nanog knockdown at this time. On the other hand, the expression of Lif/Stat3 pathway molecules and of the endoderm markers including Dab2, Gata4, Gata6 and the germ cell nuclear factor was not changed by Nanog knockdown. The results of this study demonstrated that the knockdown of Nanog expression by RNA interference in P19 cells was sufficient to modulate the expression of pluripotent markers involved in the self-renewal of EC stem cells. These results provide the valuable information on potential downstream targets of Nanog and add to our understanding of the function of Nanog in P19 EC stem cells.
Update on LPI Certification for Linux Professionals (2000)

Need to be Linux certified to get that dream job? The LPI provides the means to obtain certification.
Ethical usage of Internet facility by Students of Information science at Jimma University (2016)

This research on ethical usage of internet facility by the students of information science, Jimma University has been conducted in order to find out the ethical attitude of information science students towards usage of internet facilities, ethical advantage students had gained through the usage of internet facilities and to find out the websites they were using in the computer laboratories. Cross Sectional Survey Method was used for conducting the study. Primary and secondary data collection methods were used in this research. The instruments for data collections were observations, interviews and questionnaires and a descriptive analysis has been used for interpretation of data. Results show that ethical violations of internet happened in the night session of computer laboratory. They need to improve the ethical behavior and skill since some of the students were not ethical while using internet facilities. Saving time, paving ways for using academic or education sites and improving learning skills were some of the advantages of ethical usage of internet facilities. However lack of responsibilities while digging or using internet facility is the leading problem or obstacle to the department of information science. Some of the reasons were lack of ethical courses, shortage of panel discussion on the issues and club participation in lower classes. Students mostly use the search engines like Google, Ask, Yahoo, YouTube and so on which is followed by the social media (Facebook, Twitter and Google+). Based on the research findings, several suggestions for improvements are given at the end.
Patterning of the mesoderm in Xenopus: dose-dependent and synergistic effects of Brachyury and Pintallavis (1995)

Widespread expression of the DNA-binding protein Brachyury in Xenopus animal caps causes ectopic mesoderm formation. In this paper, we first show that two types of mesoderm are induced by different concentrations of Brachyury. Animal pole explants from embryos injected with low doses of Xbra RNA differentiate into vesicles containing mesothelial smooth muscle and mesenchyme. At higher concentrations somitic muscle is formed. The transition from smooth muscle formation to that of somitic muscle occurs over a two-fold increase in Brachyury concentration. Brachyury is required for differentiation of notochord in mouse and fish embryos, but even the highest concentrations of Brachyury do not induce this tissue in Xenopus animal caps. Co-expression of Brachyury with the secreted glycoprotein noggin does cause notochord formation, but it is difficult to understand the molecular basis of this phenomenon without knowing more about the noggin signal transduction pathway. To overcome this difficulty, we have now tested mesoderm-specific transcription factors for the ability to synergize with Brachyury. We find that co-expression of Pintallavis, but not goosecoid, with Brachyury causes formation of dorsal mesoderm, including notochord. Furthermore, the effect of Pintallavis, like that of Brachyury, is dose-dependent: a two-fold increase in Pintallavis RNA causes a transition from ventral mesoderm formation to that of muscle, and a further two-fold increase induces notochord and neural tissue. These results suggest that Pintallavis cooperates with Brachyury to pattern the mesoderm in Xenopus.
Elevated serum levels of cyclo (His-Pro), an endogenous inhibitor of pituitary prolactin secretion, in systemic lupus erythematosus patients (1997)

Cyclo (His-Pro) or CHP is a cyclic dipeptide that is known to elicit many biologic activities including inhibition of pituitary prolactin (PRL) secretion. PRL stimulates humoral and cell mediated immune responses and hyperprolactinemia has been found in a subset of systemic lupus erythematosus (SLE) patients in association with active disease. To explore whether CHP may play a role in SLE patients, we measured, CHP and PRL levels in sera of 21 SLE patients and 11 normal controls. The results of this study show significantly (P < 0.001) high levels of CHP in SLE compared to controls. However, there was no significant correlation between serum PRL and CHP levels in SLE patients (r = 0.027, P = 0.29). The increase in serum levels of CHP may be in an attempt to increase hypothalamic content of dopamine which in turn would decrease pituitary PRL synthesis in hyperprolactinemic SLE patients.
Inferring locks for atomic sections (2008)

Atomic sections are a recent and popular idiom to support the development of concurrent programs. Updates performed within an atomic section should not be visible to other threads until the atomic section has been executed entirely. Traditionally, atomic sections are supported through the use of optimistic concurrency, either using a transactional memory hardware, or an equivalent software emulation (STM).
Metal alkoxide modified organometallic reagents. Preparation and stability of organolithium reagents in tetrahydrofuran in the presence of magnesium 2-ethoxyethoxide (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetal alkoxide modified organometallic reagents. Preparation and stability of organolithium reagents in tetrahydrofuran in the presence of magnesium 2-ethoxyethoxideConstantinos G. Screttas and Barry R. SteeleCite this: J. Org. Chem. 1989, 54, 5, 1013–1017Publication Date (Print):March 1, 1989Publication History Published online1 May 2002Published inissue 1 March 1989https://pubs.acs.org/doi/10.1021/jo00266a005https://doi.org/10.1021/jo00266a005research-articleACS PublicationsRequest reuse permissionsArticle Views1215Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts