gebhart/patrepeval huggingface dataset:
Different functional domains of the adenovirus E1A gene are involved in regulation of host cell cycle products. (1987)

We have analyzed the cell cycle effects that different domains of the adenovirus E1A proteins have on quiescent primary BRK cells. Studies with deletion mutants that in combination removed all but the N-terminal 85 amino acids common to both the 12S and 13S proteins suggest that this region may be sufficient for the induction of synthesis of proliferating cell nuclear antigen and the stimulation of DNA synthesis. A second domain also common to the N-terminal exon of the 12S and 13S proteins was required for the induction of mitosis and stimulation of proliferation of primary BRK cells. A virus containing a mutation in this region was still able to stimulate DNA synthesis efficiently. A third domain, unique to the 13S protein, was required for the accelerated activation of the cellular thymidylate synthase gene in a manner similar to the 13S-dependent stimulation of adenovirus early region genes.
Coil Design and Shielding Methods for a Magnetic Resonant Wireless Power Transfer System (2013)

In this paper, we introduce the basic principles of wireless power transfer using magnetic field resonance and describe techniques for the design of a resonant magnetic coil, the formation of a magnetic field distribution, and electromagnetic field (EMF) noise suppression methods. The experimental results of wireless power transfer systems in consumer electronics applications are discussed in terms of issues related to their efficiency and EMF noise. Furthermore, we present a passive shielding method and a magnetic field cancellation method using a reactive resonant current loop and the utilization of these methods in an online electric vehicle (OLEV) system, in which an OLEV green transportation bus system absorbs wireless power from power cables underneath the road surface with only a minimal battery capacity.
Atypical Sexual Behavior During Sleep (2002)

The atypical behaviors were related to different syndromes despite the similarity of complaints from bed partners. In most cases the disturbing and often harmful symptoms were controlled when counseling was instituted and sleep disorders were treated. In some cases treatment of seizures or psychiatric disorders was also needed. Clonazepam with simultaneous psychotherapy was the most common successful treatment combination. The addition of antidepressant or antiepileptic medications was required in specific cases.
Mismatching base-pair dependence of the kinetics of DNA-DNA hybridization studied by surface plasmon fluorescence spectroscopy (2004)

Two single-stranded DNAs consisting of complementary base pairs except for one mismatching base pair (MM1) can form double-stranded DNA by molecular recognition. This type of duplex is not as stable as that formed by MM0. In order to add to a better understanding of the physical mechanism of the hybridization and dissociation processes at sensor (chip) surfaces, we studied the kinetics of the MM1 hybridization by surface plasmon fluorescence spectroscopy. Target DNA strands labelled with a fluorescent molecule Cy5 at the 5' end and hybridizing with the surface-attached probe DNA can be excited by the strong optical field of a surface plasmon resonance mode. The emitted fluorescence can be detected with high sensitivity. The affinity of a duplex was found to depend on the chemical nature, i.e. G-G, G-T etc., and on the position of the mismatching base pair along the 15mer duplex.
PRESENCE AND BIOLOGICAL ACTIVITY OF C-TYPE NATRIURETIC PEPTIDE-DEPENDENT GUANYLATE CYCLASE-COUPLED RECEPTOR IN THE PENILE CORPUS CAVERNOSUM (1998)

These results suggest for the first time that CNP modulates the erectile smooth muscle tone of penis by predominant activation of B-subtype of NPR with augmentation of cGMP production via particulate GC.
Strain relief in linearly graded composition buffer layers: A design scheme to grow dislocation-free (&lt;105 cm−2) and unstrained epilayers (1994)

The strain relaxation in linearly graded composition InGaAs layers grown on (001) GaAs substrates by molecular beam epitaxy is studied by transmission electron microscopy (TEM) and double crystal x-ray diffraction (DCXRD). The dislocation distribution in these layers does not coincide with the predicted equilibrium dislocation distribution [J. Tersoff, Appl. Phys. Lett. 62, 693 (1993)]. The dislocation density in the dislocation-rich layer thickness is slightly smaller than the equilibrium density. The thickness of the dislocation-rich region is different in the [110] and [11̄0] directions. A good correspondence exists between the TEM and DCXRD strain measurements. The dislocation distribution observed by TEM has made it possible to design a scheme to grow dislocation-free and unstrained top layers on linearly graded composition buffer layers.
Pathways of Antigen Processing (2013)

T cell recognition of antigen-presenting cells depends on their expression of a spectrum of peptides bound to major histocompatibility complex class I (MHC-I) and class II (MHC-II) molecules. Conversion of antigens from pathogens or transformed cells into MHC-I- and MHC-II-bound peptides is critical for mounting protective T cell responses, and similar processing of self proteins is necessary to establish and maintain tolerance. Cells use a variety of mechanisms to acquire protein antigens, from translation in the cytosol to variations on the theme of endocytosis, and to degrade them once acquired. In this review, we highlight the aspects of MHC-I and MHC-II biosynthesis and assembly that have evolved to intersect these pathways and sample the peptides that are produced.
Subset of T-Cell Prolymphocytic Leukemia Expresses CD117. Potential Target for Therapy. (2004)

Abstract Introduction Excluding non-hematopoietic lesions, CD117 (c-kit) is expressed by erythroid, megakaryocytic and myeloid precursors, mature mast cells, subset of plasma cell neoplasm and occasional precursor lymphoblastic tumors. C-kit plays important role in T-cell development around birth, but the expression of CD117 in mature T-cell lymphoproliferations is rare. We present flow cytometry phenotypic data of 28 cases of T-cell prolymphocytic leukemia (T-PLL). Material and methods Multiparameter 4-color FC analysis was performed on peripheral blood and/or bone marrow aspirate samples using the following antibodies: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD13, CD14, CD16, CD19, CD20, CD25, CD33, CD34, CD38, CD45, CD56, CD64, CD79a, CD117, TCRab/γδ, TdT, and HLA-DR. Fresh bone marrow aspirates and/or peripheral blood films and cytospin preparations from FC samples were stained with Wright-Giemsa. Results T-PLL was diagnosed 28 cases. The ages ranged from 43 to 92 years. Normal expression of pan-T cell antigens (CD2, CD3, CD5, CD7) was present in 57%. Abnormal expression of one, two and three antigens was noted in 21%, 11% and 11%, respectively. 4 cases showed aberrant expression of CD117. Those cases were always CD8-positive (50% of T-PLL were CD4+). There were no pan-myeloid (CD13, CD33) and blastic (TdT, CD34) markers present. Molecular studies confirmed clonal rearrangement of TCR. Discussion T-PLL is rare mature T-cell neoplasm characterized by marked lymphocytosis of small to medium-sized cells with conspicuous nucleoli. The response to conventional chemotherapy in T-PLL, similarly to other mature T-cell lymphoproliferations (except ALK+ anaplastic large cell lymphoma) generally is poor. While the use of alemtuzumab (Campath-1H) has improved remissions, the disease remains incurable. Novel treatment approaches, tailored to individual patients, and based on pathologic, phenotypic and molecular features will be crucial to improve survival for the patients with this disease. Based on the flow cytometry analysis of 28 cases of T-PLL we identified 4 cases with co-expression of CD117 (14%). When present, it is restricted to CD8+ tumors. We speculate that the expression of CD117 (c-kit) in these patients apart from its diagnostic utility, may serve as a criterion for a combination therapy with Campath-1H and imatinib mesylate.
Lactobacilli-Expressed Single-Chain Variable Fragment (scFv) Specific for Intercellular Adhesion Molecule 1 (ICAM-1) Blocks Cell-Associated HIV-1 Transmission across a Cervical Epithelial Monolayer (2006)

The vaginal and cervical epithelia provide an initial barrier to sexually acquired HIV-1 infection in women. To study the interactions between HIV-1-infected cells or cell-free HIV-1 and the reproductive epithelium, the transmission of HIV-1 by infected cells or cell-free virus across human cervical epithelial cells was examined using a Transwell culture system. Cell-associated HIV-1 was transmitted more efficiently than cell-free virus, and monocyte-associated virus was transmitted most efficiently. Abs to ICAM-1 added to the apical side of the epithelium blocked cell-mediated transepithelial HIV-1 transmission in vitro. When used in a previously described model of vaginal HIV-1 transmission in human PBL-SCID mice, anti-murine ICAM-1 Abs (0.4 microg/10 microl) also blocked vaginal transmission of cell-associated HIV-1 in vivo. To evaluate a candidate delivery system for the use of this Ab as an anti-HIV-1 microbicide, anti-ICAM single-chain variable fragment Abs secreted by transformed lactobacilli were evaluated for their protective efficacy in the Transwell model. Like the intact Ab and Fab derived from it, the single-chain variable fragment at a concentration of 6.7 microg/100 microl was able to reduce HIV-1 transmission by 70 +/- 5%. These data support the potential efficacy of an anti-ICAM Ab delivered by lactobacilli for use as an anti-HIV-1 microbicide.
A VLC media player plugin enabling dynamic adaptive streaming over HTTP (2011)

This paper describes the implementation of a VLC media player plugin enabling Dynamic Adaptive Streaming over HTTP (DASH). DASH is an emerging ISO/IEC MPEG and 3GPP standard for HTTP streaming. It aims to standardize formats enabling segmented progressive download by exploiting existing Internet infrastructure as such. Our implementation of these formats as described in this paper is based on the well-known VLC. Hence, it is fully integrated into the VLC structure and has been also submitted to the VLC development team for consideration in future releases of VLC. Therefore, it is licensed under the GNU Lesser General Public License (LGPL). The plugin provides a very flexible structure that could be easily extended with respect to different adaptation logics or profiles of the DASH standard. As a consequence, the plugin enables the integration of a variety of adaptation logics and comparison thereof, making it attractive for the research community.
Targeted Antiproliferative Drug Delivery to Vascular Smooth Muscle Cells With a Magnetic Resonance Imaging Nanoparticle Contrast Agent (2002)

These data suggest that targeted paramagnetic nanoparticles may provide a novel, MRI-visualizable, and quantifiable drug delivery system for the prevention of restenosis after angioplasty.
The role of type I interferons in non‐viral infections (2004)

For a long time, the family of type I interferons (IFN-alpha/beta) has received little attention outside the fields of virology and tumor immunology. In recent years, IFN-alpha/beta regained the interest of immunologists, due to the phenotypic and functional characterization of IFN-alpha/beta-producing cells, the definition of novel immunomodulatory functions and signaling pathways of IFN-alpha/beta, and the observation that IFN-alpha/beta not only exerts antiviral effects but is also relevant for the pathogenesis or control of certain bacterial and protozoan infections. This review summarizes the current knowledge on the production and function of IFN-alpha/beta during non-viral infections in vitro and in vivo.
A C‐terminal translocation signal is necessary, but not sufficient for type IV secretion of the Helicobacter pylori CagA protein (2006)

Type IV secretion systems are increasingly recognized as important virulence determinants of Gram-negative bacterial pathogens. While the examination of several type IV-secreted proteins suggested that their secretion depends on C-terminal signals, the nature of these signals and their conservation among different systems remain unclear. Here, we have characterized the secretion signal of the Helicobacter pylori CagA protein, which is translocated by the Cag type IV secretion apparatus into eucaryotic cells. The production of fusion proteins of CagA and green fluorescent protein (GFP) did not result in translocation of GFP to epithelial cells, but a fusion of GFP with the CagA C-terminus exerted a dominant-negative effect upon wild-type CagA translocation. We show that CagA translocation depends on the presence of its 20 C-terminal amino acids, containing an array of positively charged residues. Interestingly, these positive charges are neither necessary nor sufficient for CagA translocation, but replacing the C-terminal region of CagA with that of other type IV-secreted proteins reconstitutes CagA translocation competence. Using a novel type IV translocation assay with a phosphorylatable peptide tag, we show that removal of the N-terminal part of the CagA protein renders the protein translocation-incompetent as well. Thus, the Cag type IV secretion system seems to diverge from other systems not only with respect to its composition and architecture, but also in terms of substrate recognition and transport.
A 2.4-$\hbox{V} _{\rm pp}$ 60-Gb/s CMOS Driver With Digitally Variable Amplitude and Pre-Emphasis Control at Multiple Peaking Frequencies (2011)

The design of a 60-Gb/s CMOS driver with input signal retiming is analyzed theoretically and validated experimentally. The output stage employs a modified distributed amplifier (DA) architecture with summation of both low-pass and reactively coupled bandpass signal paths along a 50-Ω output transmission line. The DA features digital variable gain amplifier (DVGA) cells to achieve broadband waveshape control with adjustable pre-emphasis at three different peaking frequencies. Binary-weighted MOSFET gate-finger groupings are employed in a Gilbert-cell based DVGA topology to minimize bit-dependent output impedance and group delay variations. <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">S</i> -parameter measurements of the retimed driver show 54-dB gain, while the standalone DA exhibits approximately 10 dB of peaking control in each of the three frequency bands. Input and output return loss is better than -10 dB up to 60 GHz. The circuit operates from 1.2- and 2-V supplies and achieves a throughput efficiency of 12.2 mW/Gb/s. Equalization experiments at 40 Gb/s demonstrate compensation of various channel characteristics, including over 12 feet of cascaded coaxial cables with 21 dB loss at 20 GHz.
Optimization of Performance Parameter Design and Energy Use Prediction for Nearly Zero Energy Buildings (2018)

Optimizing key parameters with energy consumption as the control target can minimize the heating and cooling needs of buildings. In this paper we focus on the optimization of performance parameters design and the prediction of energy consumption for nearly Zero Energy Buildings (nZEB). The optimal combination of various performance parameters and the Energy Saving Ratio (ESR)are studied by using a large volume of simulation data. Artificial neural networks (ANNs) are applied for the prediction of annual electrical energy consumption in a nearly Zero Energy Building designs located in Shenyang (China). The data of the energy demand for our test is obtained by using building simulation techniques. The results demonstrate that the heating energy demand for our test nearly Zero Energy Building is 17.42 KW·h/(m2·a). The Energy Saving Ratio of window-to-wall ratios optimization is the most obvious, followed by thermal performance parameters of the window, and finally the insulation thickness. The maximum relative error of building energy consumption prediction is 6.46% when using the artificial neural network model to predict energy consumption. The establishment of this prediction method enables architects to easily and accurately obtain the energy consumption of buildings during the design phase.
Only dfl16, dsp2, and dq52 gene families exist in mouse immunoglobulin heavy chain diversity gene loci, of which dfl16 and dsp2 originate from the same primordial dh gene (1989)

In mice, 12 germ-line DH genes belonging to three different families (DQ52, DSP2 and DFL16) have been identified. The DH genes other than DQ52 are clustered in the 60 kb-long region located between VH and JH genes. Since there are seven DH gene families (DHQ52, DXP, DA, DK, DN, DM and DLR) in humans, we tried to identify new DH gene families in the 60 kb-long region using human DH gene probes. Mouse and human DH genes showing the highest similarity were mouse DFL16 genes and human DA genes. Southern hybridization of the mouse clones covering the 60-kb region with human DH probes did not detect any other DH genes. Nucleotide sequence analysis of the 4.0-kb fragment containing the DFL16.1 gene confirmed this conclusion. Comparison of the 12 germ-line DH genes and more than 150 somatic DH sequences also indicated that there are not more germ-line DH genes in the mouse genome. Moreover, comparison of nucleotide sequences of DFL16.1 and DSP2.2 genes and their surrounding regions suggests that both DH gene families originate from the same primordial DH gene. Using the flanking sequences of both DH genes, the divergence date between DFL16 and DSP2 genes was estimated at around 37 million years ago.
Immunolocalization of collagen types I and III, tenascin, and fibronectin in intramembranous bone. (1991)

Structural components of the organic bone matrix were located by immunohistochemical techniques in fresh-frozen sections of normal and dysplastic bone. Fine and coarse birefringent fibers were identified as separate and distinctive features in the extracellular matrix by antibodies raised against human collagen Type III. The glycoprotein tenascin was located on a proportion of the fibers in a characteristic beaded pattern, which was absent in dysplastic bone. The fibers originated in the periosteum or in the fibrous stroma of the marrow cavity and were oriented with regard to both the spatial and the lamellar organization of the bone. The disposition and composition of the fibers suggests that they form a preliminary framework on which intramembranous bone modeling proceeds, and that the specific location of tenascin on the fibers in normal developing membrane bone may be important in determining the alignment of the bone tissue. Epitopes recognized by the collagen Type I and fibronectin antibodies were demonstrated throughout the mineralized matrix, but their incorporation into the collagen "Type III" fibers was evident only outside the mineralized matrix.
Atomic Layer Deposition of Silicon Nitride from Bis(tert-butylamino)silane and N2 Plasma (2015)

Atomic layer deposition (ALD) of silicon nitride (SiNx) is deemed essential for a variety of applications in nanoelectronics, such as gate spacer layers in transistors. In this work an ALD process using bis(tert-butylamino)silane (BTBAS) and N2 plasma was developed and studied. The process exhibited a wide temperature window starting from room temperature up to 500 °C. The material properties and wet-etch rates were investigated as a function of plasma exposure time, plasma pressure, and substrate table temperature. Table temperatures of 300-500 °C yielded a high material quality and a composition close to Si3N4 was obtained at 500 °C (N/Si=1.4±0.1, mass density=2.9±0.1 g/cm3, refractive index=1.96±0.03). Low wet-etch rates of ∼1 nm/min were obtained for films deposited at table temperatures of 400 °C and higher, similar to that achieved in the literature using low-pressure chemical vapor deposition of SiNx at >700 °C. For novel applications requiring significantly lower temperatures, the temperature window from room temperature to 200 °C can be a solution, where relatively high material quality was obtained when operating at low plasma pressures or long plasma exposure times.
Purification of Steviosides by Membrane and Ion Exchange Processes (1990)

ABSTRACT An ultrafiltration (UF) process removed more than 96% of the pigments and recovered 45% of steviosides non‐nutritive sweetener from an extract of leaves. UF retentate was further processed by diafiltration (DF), and the permeate was concentrated by reverse osmosis (RO). The membrane processes (UF, DF, RO) achieved an overall steviosides recovery of more than 90% with product purity 46%. Final purification was conducted by two consecutive mixed bed ion exchange processes. The ion exchangers improved purity of the final product to 90%.
Carrots and Related Vegetable Umbelliferae (1999)

Origins, domestication, history and botany taxonomy, genetics and breeding environmental influences for crop and seed production growth and development cultural procedures pests and diseases and their management harvesting and handling postharvest procedures processing and marketing new and future directions.
Androgenic therapy for advanced breast cancer in women. A report of the cooperative breast cancer group (1973)

A randomized clinical trial of calusterone and testolactone according to the protocol of the Cooperative Breast Cancer Group (CBCG) produced objective remissions of advanced breast cancer in 28% of women receiving calusterone and 18% of those given testolactone. Effectiveness of the calusterone has prompted a dose-response study by the CBCG to determine the most effective dose of this androgen for use in treatment of breast cancer.
The Length Distribution of Class I–Restricted T Cell Epitopes Is Determined by Both Peptide Supply and MHC Allele–Specific Binding Preference (2016)

HLA class I-binding predictions are widely used to identify candidate peptide targets of human CD8(+) T cell responses. Many such approaches focus exclusively on a limited range of peptide lengths, typically 9 aa and sometimes 9-10 aa, despite multiple examples of dominant epitopes of other lengths. In this study, we examined whether epitope predictions can be improved by incorporating the natural length distribution of HLA class I ligands. We found that, although different HLA alleles have diverse length-binding preferences, the length profiles of ligands that are naturally presented by these alleles are much more homogeneous. We hypothesized that this is due to a defined length profile of peptides available for HLA binding in the endoplasmic reticulum. Based on this, we created a model of HLA allele-specific ligand length profiles and demonstrate how this model, in combination with HLA-binding predictions, greatly improves comprehensive identification of CD8(+) T cell epitopes.
Marked increase of methylumbelliferyl‐tetra‐N‐acetylchitotetraoside hydrolase activity in plasma from gaucher disease patients (1995)

Methylumbelliferyl-tetra-N-acetylchitotetraoside hydrolase activity was increased 53- to 484-fold in plasma from Gaucher disease patients and no activator could be found. High activity was also measured in other lysosomal storage diseases including Krabbe disease, Wolman disease, GM1-gangliosidosis and to a lesser extent Niemann-Pick disease type B, but the activities were lower than the lowest values in Gaucher patients. Kinetic properties of the high activity in Gaucher plasma were similar to those of controls. It is not known whether the increased activity represents intrinsic enzyme activity or increased enzyme concentration. It is possible that this enzyme may help in the detection of Gaucher disease or in the assessment of enzyme therapy with beta-D-glucosidase (Ceredase).