gebhart/patrepeval huggingface dataset:
Accelerator physics research and light source development programs at Duke University (2007)

The Duke Free-Electron Laser Laboratory (DFELL) has recently completed two major accelerator/light source development projects - we successfully commissioned the world's first distributed optical klystron FEL (DOK-1 FEL) and a new 0.24-1.2 GeV booster synchrotron. The DOK-1 FEL has a much improved FEL gain compared with two- wiggler optical klystrons. This allows the DOK-1 FEL to become a versatile light source for UV-VUV operation and as a driver for a high-flux Compton gamma-source. The top-off booster injector for the Duke storage ring is part of the upgrade project of High Intensity Gamma-ray Source (HIGS), a facility jointly developed by the DFELL and Triangle Universities Nuclear Laboratory. The accelerator and light source development has created new opportunities for accelerator physics research. In this paper, we will report our recent progress in accelerator and light source development as well as the ongoing accelerator physics research programs at the DFELL.
Integrating security in a large distributed system (1989)

Andrew is a distributed computing environment that is a synthesis of the personal computing and timesharing paradigms. When mature, it is expected to encompass over 5,000 workstations spanning the Carnegie Mellon University campus. This paper examines the security issues that arise in such an environment and describes the mechanisms that have been developed to address them. These mechanisms include the logical and physical separation of servers and clients, support for secure communication at the remote procedure call level, a distributed authentication service, a file-protection scheme that combines access lists with UNIX mode bits, and the use of encryption as a basic building block. The paper also discusses the assumptions underlying security in Andrew and analyzes the vulnerability of the system. Usage experience reveals that resource control, particularly of workstation CPU cycles, is more important than originally anticipated and that the mechanisms available to address this issue are rudimentary.
Bulk acoustic wave coupled resonator filters synthesis methodology (2005)

This paper presents a synthesis methodology applied to bulk acoustic wave coupled resonator filters (BAW CRF). BAW-CRFs are able to realize wider bandwidth and higher rejection filter responses, but they present smoother roll-off coefficients compared to classical ladder topology. The proposed methodology enables to determine the filter structure dimensions able to synthesize Chebyshev responses. Finally, we address the design of an RF filter applied to the W-CDMA system employing the proposed methodology.
AMP Deaminase Inhibitors. 3. SAR of 3-(Carboxyarylalkyl)coformycin Aglycon Analogues (2000)

N3-Substituted coformycin aglycon analogues with improved AMP deaminase (AMPDA) inhibitory potency are described. Replacement of the 5-carboxypentyl substituent in the lead AMPDA inhibitor 3-(5-carboxypentyl)-3,6,7,8-tetrahydroimidazo[4,5-d][1, 3]diazepin-8-ol (2) described in the previous article with various carboxyarylalkyl groups resulted in compounds with 10-100-fold improved AMPDA inhibitory potencies. The optimal N3 substituent had m-carboxyphenyl with a two-carbon alkyl tether. For example, 3-[2-(3-carboxy-5-ethylphenyl)ethyl]-3,6,7,8-tetrahydroimidazo[4, 5-d][1,3]diazepin-8-ol (43g) inhibited human AMPDA with a K(i) = 0. 06 microM. The compounds within the series also exhibited >1000-fold specificity for AMPDA relative to adenosine deaminase.
Unsupervised image segmentation using local homogeneity analysis (2003)

In this paper, a novel method is presented for unsupervised image segmentation based on local homogeneity analysis. First, a criterion for homogeneity of a certain pattern is proposed. Applying the criterion to local windows in the original image results in the "H-image". The high and low values of the H-image correspond to possible region boundaries and region interiors respectively. Then, a region growing method is used to segment the image based on the H-image. Finally, visually similar regions are merged together to avoid over-segmentation. Experimental results on real images show the effectiveness and robustness of the method.
Am Evaluation of Prostate-Specific Antigen in the Diagnosis and Treatment of Prostate Cancer (1987)

No AccessJournal of Urology1 Jun 1987Am Evaluation of Prostate-Specific Antigen in the Diagnosis and Treatment of Prostate Cancer Thomas A. Stamey, Alan R. Hay, John E. McNeal, and Fuad S. Freiha Thomas A. StameyThomas A. Stamey More articles by this author , Alan R. HayAlan R. Hay More articles by this author , John E. McNealJohn E. McNeal More articles by this author , and Fuad S. FreihaFuad S. Freiha More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)75517-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Am Evaluation of Prostate-Specific Antigen in the Diagnosis and Treatment of Prostate Cancer." The Journal of Urology, 137(6), p. 195A © 1987 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByGoldrath D and Messing E (2018) Prostate Specific Antigen: Not Detectable Despite Tumor Progression after Radical ProstatectomyJournal of Urology, VOL. 142, NO. 4, (1082-1084), Online publication date: 1-Oct-1989.Stamey T (2018) Editorial CommentJournal of Urology, VOL. 139, NO. 4, (764-765), Online publication date: 1-Apr-1988. Volume 137Issue 6June 1987Page: 195A Advertisement Copyright & Permissions© 1987 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Thomas A. Stamey More articles by this author Alan R. Hay More articles by this author John E. McNeal More articles by this author Fuad S. Freiha More articles by this author Expand All Advertisement PDF DownloadLoading ...
Serum Amyloid P Therapeutically Attenuates Murine Bleomycin-Induced Pulmonary Fibrosis via Its Effects on Macrophages (2010)

Macrophages promote tissue remodeling but few mechanisms exist to modulate their activity during tissue fibrosis. Serum amyloid P (SAP), a member of the pentraxin family of proteins, signals through Fcgamma receptors which are known to affect macrophage activation. We determined that IPF/UIP patients have increased protein levels of several alternatively activated pro-fibrotic (M2) macrophage-associated proteins in the lung and monocytes from these patients show skewing towards an M2 macrophage phenotype. SAP therapeutically inhibits established bleomycin-induced pulmonary fibrosis, when administered systemically or locally to the lungs. The reduction in aberrant collagen deposition was associated with a reduction in M2 macrophages in the lung and increased IP10/CXCL10. These data highlight the role of macrophages in fibrotic lung disease, and demonstrate a therapeutic action of SAP on macrophages which may extend to many fibrotic indications caused by over-exuberant pro-fibrotic macrophage responses.
The Covalent and Three-Dimensional Structure of Concanavalin A (1972)

The tentative amino-acid sequence and three-dimensional structure of the lectin concanavalin A have been determined. The amino-acid sequence, which was determined chemically, contains 238 residues. The sequences of three short stretches were assigned on the basis of x-ray crystallographic data. Interpretation of an electron density map at 2-A resolution indicates that the predominant structural element is extended polypeptide chain arranged in two anti-parallel pleated sheets or beta-structures. Residues not included in the beta-structures are arranged in regions of random coil. One of the pleated sheets contributes extensively to the interactions among the monomers to form both dimers and tetramers. The positions at which Mn(2+), Ca(2+), and saccharide are bound to the protein, and the point of cleavage for the formation of the naturally occurring fragments A(1) and A(2), have been tentatively assigned. Both metal-binding sites are at least 20-A removed from the position at which saccharides are bound. The saccharide-binding site is a deep pocket of approximately 6A x 7.5A x 18A, the inner portion of which is occupied by hydrophobic residues.
High-resolution aliasing-free optical beam steering (2016)

Many applications, including laser (LIDAR) mapping, free-space optical communications, and spatially resolved optical sensors, demand compact, robust solutions to steering an optical beam. Fine target addressability (high steering resolution) in these systems requires simultaneously achieving a wide steering angle and a small beam divergence, but this is difficult due to the fundamental trade-offs between resolution and steering range. So far, to our knowledge, chip-based two-axis optical phased arrays have achieved a resolution of no more than 23 resolvable spots in the phased-array axis. Here we report, using non-uniform emitter spacing on a large-scale emitter array, a dramatically higher-performance two-axis steerable optical phased array fabricated in a 300 mm CMOS facility with over 500 resolvable spots and 80° steering in the phased-array axis (measurement limited) and a record small divergence in both axes (0.14°). Including the demonstrated steering range in the other (wavelength-controlled) axis, this amounts to two-dimensional beam steering to more than 60,000 resolvable points.
The Role of the Leukemia Inhibitory Factor (LIF) — Pathway in Derivation and Maintenance of Murine Pluripotent Stem Cells (2011)

Developmental biology, regenerative medicine and cancer biology are more and more interested in understanding the molecular mechanisms controlling pluripotency and self-renewal in stem cells. Pluripotency is maintained by a synergistic interplay between extrinsic stimuli and intrinsic circuitries, which allow sustainment of the undifferentiated and self-renewing state. Nevertheless, even though a lot of efforts have been made in the past years, the precise mechanisms regulating these processes remain unclear. One of the key extrinsic factors is leukemia inhibitory factor (LIF) that is largely used for the cultivation and derivation of mouse embryonic and induced pluripotent stem cells. LIF acts through the LIFR/gp130 receptor and activates STAT3, an important regulator of mouse embryonic stem cell self-renewal. STAT3 is known to inhibit differentiation into both mesoderm and endoderm lineages by preventing the activation of lineage-specific differentiation programs. However, LIF activates also parallel circuitries like the PI3K-pathway and the MEK/ERK-pathway, but its mechanisms of action remain to be better elucidated. This review article aims at summarizing the actual knowledge on the importance of LIF in the maintenance of pluripotency and self-renewal in embryonic and induced pluripotent stem cells.
Genetic Polymorphism of a Bovine t-Complex Gene (TCP1) Linkage to Major Histocompatibility Genes (1988)

Southern blot analysis of genomic cattle DNA was carried out using murine cDNA probes representing the Tcp-1 gene of the t complex. Excellent cross-hybridization was obtained, and the probes apparently hybridized to at least two bovine TCP1 genes. Two independent restriction fragment length polymorphisms, each composed of two allelic variants, were detected; the inheritance of the restriction fragment length polymorphisms was confirmed by family data. One of the restriction fragment length polymorphisms, designated TCP1B, was evidently due to a gene duplication and was revealed with any restriction enzyme used. The duplication was found in three different cattle breeds investigated. Family segregation data indicated that TCP1B is linked to major histocompatibility complex genes. The result was consistent with close linkage to the major histocompatibility complex class II DO beta gene, whereas a fairly high recombination frequency was indicated between TCP1B/DO beta and other major histocompatibility complex genes. The result assigns TCP1B to a bovine linkage group previously comprising major histocompatibility complex class I and class II genes and blood group locus M. The similarity between this linkage group and parts of mouse chromosome 17 (t-H-2) and human chromosome 6 (TCP1-HLA) is discussed.
Role for Rapid Dendritic Protein Synthesis in Hippocampal mGluR-Dependent Long-Term Depression (2000)

A hippocampal pyramidal neuron receives more than 10(4) excitatory glutamatergic synapses. Many of these synapses contain the molecular machinery for messenger RNA translation, suggesting that the protein complement (and thus function) of each synapse can be regulated on the basis of activity. Here, local postsynaptic protein synthesis, triggered by synaptic activation of metabotropic glutamate receptors, was found to modify synaptic transmission within minutes.
Fluorinated pyrimidines. XXXVI. Synthesis of some 2,4-substituted 5-trifluoromethylpyrimidines (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFluorinated pyrimidines. XXXVI. Synthesis of some 2,4-substituted 5-trifluoromethylpyrimidinesHeino Diringer, Tasneem A. Khwaja, and Charles HeidelbergerCite this: J. Med. Chem. 1970, 13, 1, 151–152Publication Date (Print):January 1, 1970Publication History Published online1 May 2002Published inissue 1 January 1970https://pubs.acs.org/doi/10.1021/jm00295a047https://doi.org/10.1021/jm00295a047research-articleACS PublicationsRequest reuse permissionsArticle Views161Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library. (1983)

A cDNA library of approximately equal to 9,000 members has been prepared from chicken smooth muscle mRNA by using the plasmid expression vector pUC8. Addition of Sal I and EcoRI linkers at different stages during the preparation of the cDNA resulted in a population of molecules, most of which had EcoRI linkers at the end of the cDNA that corresponded to the 5' end of the template mRNA and Sal I linkers at the end that corresponded to the 3' end of the mRNA. The cDNA molecules then were inserted into a EcoRI-Sal I-cut plasmid vector, pUC8, that contains the transcriptional and translational start sequences from the lacZ gene upstream of the EcoRI site. The sequential addition of the linkers to the cDNA ensured that most of the cDNAs were inserted into pUC8 in the proper orientation for expression. The colonies were replica plated onto nitrocellulose filters and lysed in situ with chloroform vapor. The library was screened for colonies producing products immunologically related to chicken tropomyosin by incubating the filters first with a rabbit antitropomyosin antibody and second with a 125I-labeled goat anti-rabbit IgG. Two colonies were detected that reacted specifically with the antisera. Plasmids from both clones have been partially subjected to sequence analysis; both plasmids contain cDNAs that encode tropomyosin. These protocols are potentially useful for the identification of cDNA clones for genes expressed at low levels from large cDNA expression libraries.
Mutation within TARDBP leads to Frontotemporal Dementia without motor neuron disease (2009)

It has been recently demonstrated that the 43-kDa transactive response (TAR)-DNA-binding protein (TARDBP) is the neuropathological hallmark of Frontotemporal Dementia (FTD) with ubiquitin-positive and tau-negative inclusions. Large series of FTD patients without motor neuron disease have been previously analysed, but no TARDBP mutation was identified. The aim of the present study was to evaluate whether TARDBP gene mutations may be associated with FTD. We report that a pathogenetic TARDBP mutation is causative of behavioural variant FTD (bvFTD). An aged woman in her seventies initially started to present apathy and depression associated with impairment in executive functions. The diagnosis of bvFTD (apathetic syndrome) was accomplished by three-year follow-up, and structural and functional neuroimaging. By five-years after onset, extensive electrophysiological investigations excluded subclinical motor neuron disease. In this patient, a single base substitution c.800A>G of TARDBP gene was identified. This mutation, already described as causative of ALS, predicted the amino acidic change arginine to serine at position 267 (N267S). In silico analysis demonstrated that this substitution generates a new phosphorylation site, and western blot analysis on lymphoblastoid cells reported a decrease of protein expression in N267S mutation carrier. Our study suggests that TARDBP mutations can be pathogenetic of bvFTD without motor neuron disease. TARDBP screening needs to be considered in FTD cases.
KDR (VEGF Receptor 2) Is the Major Mediator for the Hypotensive Effect of VEGF (2002)

Vascular endothelial growth factor (VEGF) exerts vasodilation-induced hypotension as a major side effect for treatment of ischemic diseases. VEGF has 2 receptor tyrosine kinases, KDR and Flt-1. Little is known about which receptor mediates VEGF-induced hypotension. To elucidate the role of each receptor in mediating hypotension, KDR-selective and Flt-1-selective mutants were used for in vitro and in vivo studies. The KDR-selective mutant induced vascular endothelial cell proliferation comparable to VEGF, whereas the Flt-1- selective mutant had no effect on proliferation. Intravenous injection of KDR-selective mutant, Flt-selective mutant, or VEGF caused a dose-related decrease in mean arterial pressure in conscious rats. The hypotensive response to KDR-selective mutant was significantly less than that to VEGF (P<0.01) but was greater than that to Flt-selective mutant (P<0.01). Similarly, VEGF and KDR-selective mutant induced more potent vasorelaxation than Flt-selective mutant or placenta growth factor that binds Flt-1 only (P<0.01), and the vasorelaxation to KDR-selective mutant was not significantly different at low concentrations but less than that to VEGF at high concentrations. The results indicate that the vasodilation and hypotensive effect of VEGF may involve both receptors, but KDR is the predominant receptor mediating this effect. Because KDR-selective mutant induced proliferation and angiogenesis similar to VEGF but was associated with 36% attenuation in hypotension, the data suggest that the KDR-selective mutant may represent an alternative treatment for ischemic diseases.
A Comparison of Renal Preservation by Cold Storage and Machine Perfusion Using a Porcine Autotransplant Model (2004)

In this model, MP improved 24-hr preservation of kidneys not subjected to warm ischemia (heart-beating donor model), but there was no evidence that MP was a better method of preservation than CS for kidneys exposed to 30 min of WIT (NHBD model).
Towards universal systems for recombinant gene expression (2010)

Recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. Today, two recombinant expression systems are particularly well represented in the literature reporting on recombinant expression of specific genes. According to searches in the PubMed citation database, during the last 15 years 80% of all recombinant genes reported on in the literature were expressed in either the enterobacterium Escherichia coli or the methylotropic yeast Pichia pastoris. Nevertheless, some eukaryotic proteins are misfolded or inadequately posttranslationally modified in these expression systems. This situation demands identification of other recombinant expression systems that enable the proper expression of the remaining eukaryotic genes. As of now, a single universal system allowing expression of all target genes is still a distant goal. In this light, thorough experimental screening for systems that can yield satisfying quantity and quality of target protein is required. In recent years, a number of new expression systems have been described and used for protein production. Two systems, namely Drosophila melanogaster S2 insect cells and human embryonic kidney 293 (HEK293) cells stably expressing the EBNA-1 gene, show exceptional promise. The time has come to identify a few well-performing systems that will allow us to express, purify, and characterize entire eukaryotic genomes.
Organic Synthesis on Soluble Polymer Supports:  Liquid-Phase Methodologies (1997)

ADVERTISEMENT RETURN TO ISSUEPREVArticleOrganic Synthesis on Soluble Polymer Supports: Liquid-Phase MethodologiesDennis J. Gravert and Kim D. JandaView Author Information The Scripps Research Institute, Department of Chemistry and The Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, California 92037 Cite this: Chem. Rev. 1997, 97, 2, 489–510Publication Date (Web):April 1, 1997Publication History Received28 August 1996Revised13 January 1997Published online1 April 1997Published inissue 1 April 1997https://doi.org/10.1021/cr960064lCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views5144Altmetric-Citations517LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (769 KB) Get e-AlertscloseSUBJECTS:Alcohols,Biopolymers,Crystal cleavage,Peptides and proteins,Polymers Get e-Alerts