gebhart/patrepeval huggingface dataset:
Synthesis of enantiomerically pure (2'R,5'S)-(-)-1-(2-hydroxymethyloxathiolan-5-yl)cytosine as a potent antiviral agent against hepatitis B virus (HBV) and human immunodeficiency virus (HIV) (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of enantiomerically pure (2'R,5'S)-(-)-1-(2-hydroxymethyloxathiolan-5-yl)cytosine as a potent antiviral agent against hepatitis B virus (HBV) and human immunodeficiency virus (HIV)J. Warren Beach, Lak S. Jeong, Antonio J. Alves, Douglas Pohl, Hea O. Kim, Chien Neng Chang, Shin Lian Doong, Raymond F. Schinazi, Yung Chi Cheng, and Chung K. ChuCite this: J. Org. Chem. 1992, 57, 8, 2217–2219Publication Date (Print):April 1, 1992Publication History Published online1 May 2002Published inissue 1 April 1992https://pubs.acs.org/doi/10.1021/jo00034a006https://doi.org/10.1021/jo00034a006research-articleACS PublicationsRequest reuse permissionsArticle Views842Altmetric-Citations185LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Developmental Changes in the Basement Membrane of the Normal and Hypothyroid Postnatal Rat Testis: Segmental Localization of Fibulin-2 and Fibronectin1 (1998)

To characterize developmental changes in the extracellular matrix of the postnatal rat testis in relationship to the timing of germ cell maturation, we immunolocalized fibulin-1, fibulin-2, and other matrix components in the testes of normal and propyl-thiouracil (PTU)-induced hypothyroid animals. Unlike laminin, nidogen, and perlecan, which were present in the seminiferous tubule basement membrane (BM) throughout postnatal development, fibulins were found to disappear from the postnatal tubule BM. Fibulin-1 was no longer detected after Day 5 whereas fibulin-2 became localized in a segmental manner within the BM of each seminiferous tubule on Days 10 and 15 and disappeared by Day 20. Fibronectin showed a segmental pattern in the level of immunostaining of the tubule BM on Days 10 and 15, with a more uniform staining seen at earlier and later ages. Collagen VI was initially confined to the interstitial matrix in the Day 5 testis and became progressively more closely associated with the seminiferous tubule BM at later stages. The disappearance of fibulin-2 and the BM-association of collagen VI were both delayed in the PTU-treated testes. The developmental changes in the staining patterns for fibulin-2 and fibronectin coincide with the adhesion and alignment of peritubular cells on the inner seminiferous tubule BM. The delay in maturation of the seminiferous tubule BM in the testes of PTU-treated rats demonstrates a correlation between changes in the composition of the tubule BM and cellular development of the testis.
Structural Logistic Regression for Link Analysis (2003)

We present Structural Logistic Regression, an extension of logistic regression to modeling relational data. It is an integrated approach to building regression models from data stored in relational databases in which potential predictors, both boolean and real-valued, are generated by structured search in the space of queries to the database, and then tested with statistical information criteria for inclusion in a logistic regression. Using statistics and relational representation allows modeling in noisy domains with complex structure. Link prediction is a task of high interest with exactly such characteristics. Be it in the domain of scientific citations, social networks or hypertext, the underlying data are extremely noisy and the features useful for prediction are not readily available in a &amp;quot;flat&amp;quot; file format. We propose the application of Structural Logistic Regression to building link prediction models, and present experimental results for the task of predicting citations made in scientific literature using relational data taken from the CiteSeer search engine. This data includes the citation graph, authorship and publication venues of papers, as well as their word content.
Pharmacology of exenatide (synthetic exendin-4) for the treatment of type 2 diabetes. (2003)

New therapies for the long-term treatment of type 2 diabetes are needed to ameliorate declining pancreatic beta-cell function. Ideally, these therapies should lower fasting and post-prandial blood glucose, produce no hypoglycemia or weight gain, cause no other limiting side effects, and reduce cardiovascular complications. Exenatide (synthetic exendin-4) is a potential therapeutic which may fulfill these criteria. Dose-ranging studies have identified an optimal dose of 0.05 to 0.2 microgram/kg administered subcutaneously twice daily. Pharmacokinetic data support a pivotal study design which mitigates the transient nausea observed in early studies by including a dose initiation period of 1 month at 5 micrograms twice daily, followed by maintenance therapy at 10 micrograms twice daily. Ongoing studies suggest exenatide improves glycemic control through a combination of mechanisms discussed in this review.
Studies on the Internalization Mechanism of Cationic Cell-penetrating Peptides (2003)

A great deal of data has been amassed suggesting that cationic peptides are able to translocate into eucaryotic cells in a temperature-independent manner. Although such peptides are widely used to promote the intracellular delivery of bioactive molecules, the mechanism by which this cell-penetrating activity occurs still remains unclear. Here, we present an in vitro study of the cellular uptake of peptides, originally deriving from protegrin (the SynB peptide vectors), that have also been shown to enhance the transport of drugs across the blood-brain barrier. In parallel, we have examined the internalization process of two lipid-interacting peptides, SynB5 and pAntp-(43-58), the latter corresponding to the translocating segment of the Antennapedia homeodomain. We report a quantitative study of the time- and dose-dependence of internalization and demonstrate that these peptides accumulate inside vesicular structures. Furthermore, we have examined the role of endocytotic pathways in this process using a variety of metabolic and endocytosis inhibitors. We show that the internalization of these peptides is a temperature- and energy-dependent process and that endosomal transport is a key component of the mechanism. Altogether, our results suggest that SynB and pAntp-(43-58) peptides penetrate into cells by an adsorptive-mediated endocytosis process rather than temperature-independent translocation.
Comparison of self-reported fatigue in rheumatoid arthritis and controls. (1995)

The importance of assessing fatigue in RA is confirmed. Effective management is needed to control clinical manifestations of RA that were found to be strongly associated with fatigue such as poor sleep, functional disability, pain, depressive symptoms, and lower Hct.
Transactivation of programmed ribosomal frameshifting by a viral protein (2014)

Programmed -1 ribosomal frameshifting (-1 PRF) is a widely used translational mechanism facilitating the expression of two polypeptides from a single mRNA. Commonly, the ribosome interacts with an mRNA secondary structure that promotes -1 frameshifting on a homopolymeric slippery sequence. Recently, we described an unusual -2 frameshifting (-2 PRF) signal directing efficient expression of a transframe protein [nonstructural protein 2TF (nsp2TF)] of porcine reproductive and respiratory syndrome virus (PRRSV) from an alternative reading frame overlapping the viral replicase gene. Unusually, this arterivirus PRF signal lacks an obvious stimulatory RNA secondary structure, but as confirmed here, can also direct the occurrence of -1 PRF, yielding a third, truncated nsp2 variant named "nsp2N." Remarkably, we now show that both -2 and -1 PRF are transactivated by a protein factor, specifically a PRRSV replicase subunit (nsp1β). Embedded in nsp1β's papain-like autoproteinase domain, we identified a highly conserved, putative RNA-binding motif that is critical for PRF transactivation. The minimal RNA sequence required for PRF was mapped within a 34-nt region that includes the slippery sequence and a downstream conserved CCCANCUCC motif. Interaction of nsp1β with the PRF signal was demonstrated in pull-down assays. These studies demonstrate for the first time, to our knowledge, that a protein can function as a transactivator of ribosomal frameshifting. The newly identified frameshifting determinants provide potential antiviral targets for arterivirus disease control and prevention. Moreover, protein-induced transactivation of frameshifting may be a widely used mechanism, potentially including previously undiscovered viral strategies to regulate viral gene expression and/or modulate host cell translation upon infection.
Combination immunotherapy: a road map (2017)

Cancer immunotherapy and in particular monoclonal antibodies blocking the inhibitory programed cell death 1 pathway (PD-1/PD-L1) have made a significant impact on the treatment of cancer patients in recent years. However, despite the remarkable clinical efficacy of these agents in a number of malignancies, it has become clear that they are not sufficiently active for many patients. Initial evidence, for example with combined inhibition of PD-1 and CTLA-4 in melanoma and non-small cell lung cancer (NSCLC), has highlighted the potential to further enhance the clinical benefits of monotherapies by combining agents with synergistic mechanisms of action. In order to address the current progress and consider challenges associated with these novel approaches, the Society for Immunotherapy of Cancer (SITC) convened a Combination Immunotherapy Task Force. This Task Force was charged with identifying and prioritizing the most promising prospects for combinatorial approaches as well as addressing the challenges associated with developing these strategies. As a result of the extensive clinical benefit and tolerable side effects demonstrated with agents inhibiting the PD-1 pathway, an overview of current evidence to support its promising potential for use as a backbone in combination strategies is presented. In addition, key issues in the development of these strategies including preclinical modeling, patient safety and toxicity considerations, clinical trial design, and endpoints are also discussed. Overall, the goal of this manuscript is to provide a summary of the current status and potential challenges associated with the development and clinical implementation of these strategies.
Marrow Stromal Cell Culture (1998)

The first book of its kind on marrow stromal cells, and written by leading experts in the field, this concise handbook aims to provide all that is needed to those new to this relatively young area of research. It provides a historical perspective and a summary of present knowledge of the marrow stromal lineages which are currently best understood. Detailed protocols for the isolation, culture and characterization of marrow stromal cell types from human and other animal species are given. The breadth and depth of the subject matter included makes the book equally suitable for new researchers and the more experienced investigator in this rapidly expanding field, including clinicians and bioscientists.
Synthesis and anticancer activity of nitrosourea derivatives of phensuximide (1980)

Nitrosourea derivatives 9--13 which utilize phensuximide (1) as the carrier were synthesized as potential central nervous system antitumor agents. The N-(2-chloroethyl)-N-nitrosourea 13 was active in the mouse ependymoblastoma brain-tumor system, as well as the intraperitoneal L1210 leukemia system.
Mesothelin (2004)

Mesothelin is a differentiation antigen present on normal mesothelial cells and overexpressed in several human tumors, including mesothelioma and ovarian and pancreatic adenocarcinoma. The mesothelin gene encodes a precursor protein that is processed to yield the 40-kDa protein, mesothelin, attached to the cell membrane by a glycosylphosphatidyl inositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor. The biological function of mesothelin is not known. Mesothelin is a promising candidate for tumor-specific therapy, given its limited expression in normal tissues and high expression in several cancers. SS1(dsFv)PE38 is a recombinant anti-mesothelin immunotoxin that is undergoing clinical evaluation in patients with mesothelin-expressing tumors. There is evidence that mesothelin is an immunogenic protein and could be exploited as a therapeutic cancer vaccine. A soluble mesothelin variant has been identified and could be a useful tumor marker for malignant mesotheliomas.
The ATP Synthase atpHAGDC (F 1 ) Operon from Rhodobacter capsulatus (1998)

The atpHAGDC operon of Rhodobacter capsulatus, containing the five genes coding for the F1 sector of the ATP synthase, has been cloned and sequenced. The promoter region has been defined by primer extension analysis. It was not possible to obtain viable cells carrying atp deletions in the R. capsulatus chromosome, indicating that genes coding for ATP synthase are essential, at least under the growth conditions tested. We were able to circumvent this problem by combining gene transfer agent transduction with conjugation. This method represents an easy way to construct strains carrying mutations in indispensable genes.
Shark: SQL and Rich Analytics at Scale (2012)

Shark is a new data analysis system that marries query processing with complex analytics on large clusters. It leverages a novel distributed memory abstraction to provide a unified engine that can run SQL queries and sophisticated analytics functions (e.g., iterative machine learning) at scale, and efficiently recovers from failures mid-query. This allows Shark to run SQL queries up to 100 faster than Apache Hive, and machine learning programs up to 100 faster than Hadoop. Unlike previous systems, Shark shows that it is possible to achieve these speedups while retaining a MapReduce-like execution engine, and the fine-grained fault tolerance properties that such engines provide. It extends such an engine in several ways, including column-oriented in-memory storage and dynamic mid-query replanning, to effectively execute SQL. The result is a system that matches the speedups reported for MPP analytic databases over MapReduce, while offering fault tolerance properties and complex analytics capabilities that they lack.
THE TIME COURSE OF CUTANEOUS PORPHYRIN PHOTOSENSITIZATION IN THE MURINE EAR (1989)

This study was designed to investigate the time course of acute cutaneous photosensitivity following administration of Photofrin II using the murine ear swelling response (ESR) as an in vivo end-point. Ros:(ICR) mice were injected with 5 mg/kg Photofrin II and illuminated 7.5 h to 31 days later with 630-nm laser light; ESR was measured 24 h after illumination. There was a direct correlation between ESR and the concentration of [14C]Photofrin II in blood, while no relationship between ESR and the level of [14C]Photofrin II in the ear tissue of exsanguinated mice was evident. Photosensitivity in the mouse foot can be suppressed by preexposure to low doses of light via a photochemical destruction of tissue-bound sensitizer (Boyle and Potter, 1987, Photochem. Photobiol. 46, 997-1001). However, mouse ears pretreated with 84 J/cm2 of 630-nm light (28 J/cm2/day, given 2, 4 and 6 d after injection), a dose sufficient to reduce porphyrin fluorescence in ear tissue by about 75%, prior to the usual light dose (88.6 J/cm2, 630 nm, day 9 after injection) showed a mean ESR not significantly different (P less than 0.5) from that for ears which received only a single dose of 88.6 J/cm2 on day 9. It is concluded, for this animal model, that circulating porphyrin is the source of photoinduced ear-tissue edema and that photobleaching of tissue-bound sensitizer does not attenuate ear-tissue photosensitivity.
Purification and composition of the proteins from Sindbis virus grown in chick and BHK cells (1976)

Procedures are described for the purification of the Sindbis virus structural proteins. The amino acid and carbohydrate compositions of the purified proteins are presented for virus grown in BHK-21/13 and chicken embryo cells. Glycoprotein E1 from virus grown in BHK cells is deficient in a mannose-rich glycopeptide found on that glycoprotein when virus is grown in chicken embryo cells. The complex glactose-containing glycopeptides appear similar for virus grown in both hosts. However, when virus is grown in BHK cells, both glycoproteins are enriched in those glycopeptides containing more sialic acid. Since the two viral glycoproteins are difficult to separate cleanly during purification, it is suggested that there may be strong, but noncovalent, interactions between glycoproteins E1 and E2. It is also suggested that there may be an interaction between glycoprotein E2 and a component of the nucleocapsid.
High Rate Gas Phase  CO 2 Reduction to Ethylene and Methane Using Gas Diffusion Electrodes (1990)

La reduction chimique du dioxyde de carbone, en phase gazeuse, sur une electrode a diffusion de gaz est etudiee en solution d'hydroxyde de potassium
In Vitro Lung Models and Their Application to Study SARS-CoV-2 Pathogenesis and Disease (2021)

SARS-CoV-2 has spread across the globe with an astonishing velocity and lethality that has put scientist and pharmaceutical companies worldwide on the spot to develop novel treatment options and reliable vaccination for billions of people. To combat its associated disease COVID-19 and potentially newly emerging coronaviruses, numerous pre-clinical cell culture techniques have progressively been used, which allow the study of SARS-CoV-2 pathogenesis, basic replication mechanisms, and drug efficiency in the most authentic context. Hence, this review was designed to summarize and discuss currently used in vitro and ex vivo cell culture systems and will illustrate how these systems will help us to face the challenges imposed by the current SARS-CoV-2 pandemic.
Crossing-channel waveguide electrooptic modulators (1986)

This paper presents a general theoretical investigation of the integrated-optical crossing-channel waveguide electrooptic modulator (sometimes called a "TIR" modulator). The theoretical model is based upon the analysis of mode conversion during propagation through the device. The theoretical results are compared to experimental data. The operation of these modulators can be roughly divided into two classes, reflective-type and mode-interference-type devices, based upon an "adiabatic criterion" (made up of the optical wavelength, the waveguide width, and waveguide crossing angle) and upon the number of modes supported by the waveguide.
Asymmetric Catalytic Mannich Reactions Catalyzed by Urea Derivatives:  Enantioselective Synthesis of β-Aryl-β-Amino Acids (2002)

Highly enantioselective addition reactions between silyl ketene acetals and N-Boc aldimines are catalyzed by the thiourea-based catalyst 1c. Extraordinary scope is observed in this methodology with regard to the imine substrate, with aryl and heteroaromatic derivatives generally affording nearly quantitative yields of beta-amino ester product in up to 98% enantioselectivity.
Streptococcus pneumoniae Evades Complement Attack and Opsonophagocytosis by Expressing the pspC Locus-Encoded Hic Protein That Binds to Short Consensus Repeats 8–11 of Factor H (2002)

Streptococcus pneumoniae is an important cause of upper and lower respiratory tract infections, meningitis, peritonitis, bacterial arthritis, and sepsis. Here we have studied a novel immune evasion mechanism of serotype 3 pneumococci, which are particularly resistant to phagocytosis. On their surfaces the bacteria express the factor H-binding inhibitor of complement (Hic), a protein of the pneumococcal surface protein C family. Using radioligand binding, microtiter plate assays, surface plasmon resonance analysis, and recombinant constructs of factor H, we located the binding site of Hic to short consensus repeats (SCRs) 8-11 in the middle part of factor H. This represents a novel microbial interaction region on factor H. The only other ligand known so far for SCRs 8-11 of factor H is C-reactive protein (CRP), an acute phase protein that binds to the pneumococcal C-polysaccharide. The binding sites of Hic and CRP within the SCR8-11 region were different, however, because CRP did not inhibit the binding of Hic and required calcium for binding. Binding of factor H to Hic-expressing pneumococci promoted factor I-mediated cleavage of C3b and restricted phagocytosis of pneumococci. Thus, virulent pneumococci avoid complement attack and opsonophagocytosis by recruiting functionally active factor H with the Hic surface protein. Hic binds to a previously unrecognized microbial interaction site in the middle part of factor H.
Smart Nanomaterials Responsive to Multiple Chemical Stimuli with Controllable Cooperativity (2006)

Aptamer-linked smart materials responsive to a combination of any two analytes, including adenosine, cocaine, and K+, are demonstrated. The effect of small molecules on the properties of the nanomaterials can be either highly cooperative or non-cooperative, which can be described as logic gates with chemical inputs and colorimetric output (see figure).
Pyrrolopyrimidine nucleosides. III. Total synthesis of toyocamycin, sangivamycin, tubercidin, and related derivatives (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPyrrolopyrimidine nucleosides. III. Total synthesis of toyocamycin, sangivamycin, tubercidin, and related derivativesRichard L. Tolman, Roland K. Robins, and Leroy B. TownsendCite this: J. Am. Chem. Soc. 1969, 91, 8, 2102–2108Publication Date (Print):April 1, 1969Publication History Published online1 May 2002Published inissue 1 April 1969https://pubs.acs.org/doi/10.1021/ja01036a040https://doi.org/10.1021/ja01036a040research-articleACS PublicationsRequest reuse permissionsArticle Views701Altmetric-Citations104LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts