gebhart/patrepeval huggingface dataset:
[A comparative study of the anabolic action of ecdysten, leveton and Prime Plus, preparations of plant origin]. (1996)

A new class of anabolic substances includes different saponins, originated from Leuzea rhaponticum sp. The effect of these substances on the human body was studied by caliperometric measurements of body folds. All tested substances taken orally for three weeks diminished fat content under conditions of daily aerobic-anaerobic training. Ekdisten and Prime Plus (combination of ekdisten and pure protein) elevated the muscle mass. The same effect of leventon was less pronounced. We showed that all substances studied elevated the magnitude of "total work", estimated per 1 kg of body weight. Ekdisten and Prime Plus again appeared to be most efficient. The prospects of using the saponins in sport medicine are discussed.
A Functional Recombinant Human 4-1BB Ligand for Immune Costimulatory Therapy of Cancer (2011)

Costimulatory factors hold great promise for development into novel anticancer biotherapeutics. An agonist to 4-1BB is ranked number 8 by National Cancer Institute on the list of 20 agents with high potential for use in treating cancer. We earlier reported on a recombinant murine 4-1BB ligand fusion protein that binds 4-1BB receptor on murine T cells and stimulates their proliferation in tumor-bearing mice. To facilitate clinical translation,we constructed a corresponding recombinant human 4-1BB ligand fusion protein (hIg-h4-1BBLs) and showed its ability to activate human T cells in vitro. Using Chinese hamster ovary cells transformed with a plasmid coexpressing hIg-h4-1BBLs and rat glutamine synthetase, we generated a high-producing clone by sequential selection with methionine sulfoximine. The hIg-h4-1BBLs was partially purified by protein A column chromatography and characterized biochemically and functionally, using human 4-1BB binding and human T-cell proliferation assays, in vitro.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western Blot confirmed that the hIg-h4-1BBLs is expressed predominantly as a functionally active multimeric protein with the ability to specifically bind to cells expressing human 4-1BB receptor and induce significant T-cell proliferation in vitro using both human and monkey peripheral blood mononuclear cells. The hIg-h4-1BBLs can be produced in large quantities from the high producer clone and developed as a novel immune costimulatory biotherapeutic to treat, alone and in combination with other modalities, various malignant diseases in patients through T-cell activation. Process development of this clinical agent has been discussed with the Food and Drug Administration in a pre-Investigational New Drug meeting and presented to the Office of Biotechnology Activities in a public hearing.
Glutamine‐limited batch hybridoma growth and antibody production: Experiment and model (1990)

The influence of glutamine (a major energy source) on both hybridoma growth and monoclonal antibody production was examined. A series of batch experiments were performed in T-flasks containing initial glutamine levels ranging from 0.5 to 4.0 mM in RPMI 1640 with 20% v/v fetal calf serum. The maximum final cell concentration increased with initial glutamine levels in the range of 0.5-2 mM; further glutamine increases had little or no effect. Earlier studies in our laboratories demonstrated that serum component(s) strongly influence the maximum specific growth rate. Here, the present studies reveal also the stoichiometric limitation by glutamine in the later stages of growth when its concentration is drastically reduced. For 0.5 to 1.5 mM initial glutamine, complete substrate utilization coincided with the cessation of cell growth and the onset of the death phase. For initial glutamine concentrations higher than 2.0 mM, growth halted prior to glutamine exhaustion, presumably because serum or RPMI component(s) were exhausted. The specific antibody secretion rate was essentially non-growth-associated above a critical low glutamine concentration in both the growth and death phases. At or below this critical value, an apparent emergence of stoichiometnc or energy limitation resulted in a dramatic drop in the secretion rate to zero. A simple unstructured model was developed that simulates these trends well. All parameters were determined using only subsets of the data. Nevertheless, these parameter values provided simulations in good agreement with all the glutamine-limited cultures.
Neuritic pathology and dementia in alzheimer's disease (1991)

Previous studies of Alzheimer's disease (AD) have correlated the severity of dementia with either the number of senile plaques or neurofibrillary tangles. We used antibodies raised against amyloid beta/A4 protein of senile plaque cores and tau protein as well as thioflavine S and the Campbell-Switzer modification of the Hicks silver method to examine the hippocampal formation and five neocortical regions from 22 nondemented elderly control subjects and 34 demented patients with cerebral senile plaques and neurofibrillary tangles, without complicating disease processes. Ten control subjects (46%) had no beta/A4 protein deposition. Twelve control subjects (54%) had widespread beta/A4 protein deposition but no neocortical neuritic pathology. Of the 34 patients with AD-type changes, 27 (79%) had widespread senile plaques and neurofibrillary tangles, while 7 (21%) had neocortical senile plaques with few neurofibrillary tangles. All demented patients had widespread beta/A4 protein deposition and neocortical tau-immunoreactive, Hicks silver-positive dystrophic neurites. The neurites were found both free in the neuropil as well as surrounding senile plaques. Quantitative analysis showed that dystrophic neurites were significantly increased in patients with AD compared with control subjects and the number of dystrophic neurites and neurofibrillary tangles correlated with the clinical severity of dementia. Widespread cerebral beta/A4 protein deposition may be necessary but by itself is insufficient for the development of dementia in AD.
CRF-based Bibliography Extraction from Reference Strings Focusing on Various Token Granularities (2012)

The references of academic articles include important bibliographic elements such as authors' names and article titles. Automatic extraction of these elements is useful because they can be used for various purposes, including searching. In this paper, a method for automatically extracting bibliographic elements from the text of reference strings is proposed. The proposed method assigns bibliographic labels to reference strings by using linguistic information and conditional random fields. Experimental results indicated that the extraction accuracies of major bibliographies were more than 96%.
Oncofetal protein, IMP‐3, a potential marker for prediction of postoperative peritoneal dissemination in gastric adenocarcinoma (2011)

IMP-3 expression in primary gastric tumors was an independent poor prognostic factor. IMP-3 mRNA expression in peritoneal lavage fluid was a predictor of recurrence after surgery in gastric cancer and a marker of poor prognosis.
Elevated Levels of the Vesicular Monoamine Transporter and a Novel Repetitive Behavior in the Drosophila Model of Fragile X Syndrome (2011)

Fragile X Syndrome (FXS) is characterized by mental impairment and autism in humans, and it often features hyperactivity and repetitive behaviors. The mechanisms for the disease, however, remain poorly understood. Here we report that the dfmr1 mutant in the Drosophila model of FXS grooms excessively, which may be regulated differentially by two signaling pathways. Blocking metabotropic glutamate receptor signaling enhances grooming in dfmr1 mutant flies, whereas blocking the vesicular monoamine transporter (VMAT) suppresses excessive grooming. dfmr1 mutant flies also exhibit elevated levels of VMAT mRNA and protein. These results suggest that enhanced monoamine signaling correlates with repetitive behaviors and hyperactivity associated with FXS.
Binding of α-melanocyte-stimulating hormone to its G-protein-coupled receptor on B-lymphocytes activates the Jak/STAT pathway (1998)

alpha-Melanocyte-stimulating hormone (alpha-MSH) is a 13-amino-acid peptide with a variety of physiological effects, including the stimulation of melanocyte proliferation and melanogenesis, temperature control, control of prolactin release and the modulation of cytokine action in the immune system. There are five known subtypes of G-protein-coupled receptors, which bind with different affinities to alpha-MSH. This paper provides evidence that Ba/F3 pro-B-lymphocyte cells express the gene for the melanocortin 5 (MC5) receptor and specifically bind alpha-MSH. Western-blot analysis reveals that alpha-MSH binding stimulates Janus kinase 2 (JAK2) and signal transducers and activators of transcription (STAT1) tyrosine phosphorylation in both Ba/F3 cells and human cultured IM-9 lymphocytes. alpha-MSH is further revealed to activate JAK2 in mouse L-cells stably expressing the human MC5 receptor. Finally, alpha-MSH binding is shown to result in an enhancement of cellular proliferation. These findings identify a new protein tyrosine kinase pathway in the action of alpha-MSH, and suggest that alpha-MSH plays an important role in B-lymphocyte function via the activation of the same intracellular phosphorylation pathway used by cytokines and growth factors.
Steric effects. A study of a rationally designed system (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSteric effects. A study of a rationally designed systemGarry Bott, Leslie D. Field, and Sever SternhellCite this: J. Am. Chem. Soc. 1980, 102, 17, 5618–5626Publication Date (Print):August 1, 1980Publication History Published online1 May 2002Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/ja00537a036https://doi.org/10.1021/ja00537a036research-articleACS PublicationsRequest reuse permissionsArticle Views2542Altmetric-Citations327LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Identification of attenuating mutations on the reovirus type 3 S1 double-stranded RNA segment with a rapid sequencing technique (1986)

Reovirus type 3 variants with mutations in the major neutralization domain of the sigma 1 protein have attenuated neurovirulence and restricted neurotropism. We devised a variation of the rapid RNA sequencing technique to facilitate the analysis of double-stranded RNA. We sequenced the S1 double-stranded RNA segment, which encodes the sigma 1 protein, of five attenuated reovirus type 3 variants. Four of the variants have changes in codon 419, and a fifth variant has a change at codon 340, all of which resulted in amino acid substitutions in the sigma 1 protein. We identified two sites on the reovirus type 3 sigma 1 protein that play a critical role in neurovirulence.
Treatment of intractable cancer pain by electronically controlled parenteral infusion of analgesic drugs (1993)

Intractable metastatic cancer pain can be controlled in the majority of cases with narcotic drugs. This review focuses on parenteral analgesic therapy; the available drugs, administration systems, and electronic infusion devices. The range of drugs suitable for parenteral therapy, including continuous infusion therapy by subcutaneous, intravenous, or epidural routes is limited both by pharmacokinetic and side effects profiles. Implantable ports and catheter systems permit ambulatory delivery of drugs when combined with portable electronic pumps. This enables patient care to move from a bed-bound hospital therapy to ambulation in the hospital or home. The safeguards, features, and modes of operation of these pump-port-catheter-drug systems are described.
Evidence for increased synthesis as well as increased degradation of protein kinase C after treatment of human osteosarcoma cells with phorbol ester. (1987)

Phorbol 12-myristate 13-acetate (PMA) induces time-dependent changes in protein kinase C subcellular distribution and enzymatic activity in the human osteosarcoma cell line SaOS-2. Short (less than 60 min) incubations with PMA caused decreased cytosolic enzyme activity and a concomitant increase in particulate protein kinase; after 3 h, particulate protein kinase C activity also declined to reach less than 10% of basal activity by 24 h (Krug, E., and Tashjian, Jr., A. H., (1987) Cancer Res. 47, 2243-2246). In order to determine whether the loss in enzyme activity was due to decreased enzyme protein, Western blot analyses were performed using a polyclonal antibody against protein kinase C raised in rabbits. This approach confirmed the previously reported time-related changes: 80-kDa immunoreactive protein kinase C initially translocated from the cytosol to the particulate cell fraction and later disappeared completely from the particulate fraction. Loss of protein kinase C enzymatic activity thus results from actual loss of the 80-kDa protein; we found no evidence for generation of a calcium/phospholipid-independent protein kinase C-like form of the enzyme. Membrane association was confirmed by immunoprecipitation experiments using [35S]methionine-labeled cells. Brief exposure to PMA caused a marked loss in the [35S]methionine-labeled cytosolic protein kinase C band and an increase in the labeled particulate band. Protein kinase C immunoprecipitated from cells treated with PMA for 14 h displayed an increase in [35S]methionine label despite a greater than 80% loss of enzyme activity. The high specific radioactivity of the remaining 80-kDa protein leads us to conclude that long term treatment with PMA causes an increase in the rate of protein kinase C synthesis accompanied by a still greater increase in the rate of enzyme degradation in SaOS-2 cells.
Removal of Benzyl Protecting Groups from Solid-Supported Compounds by Hydrogenolysis Using Palladium Nanoparticles (2000)

So mild und effektiv wie die konventionelle Hydrogenolyse in Lösung ist die Abspaltung von Benzylgruppen an Kohlenhydraten, die an ein festes Trägermaterial gebunden sind, mit Palladium-Nanoteilchen als Katalysatoren. Der Einsatz eines UV-aktiven Linkers, der die benzylgeschützten Glycoside von Glucose, Fucose und Lactose an den Träger bindet (siehe Bild), ermöglicht es, den Verlauf der Hydrogenolyse nach der Abspaltung vom Träger zu verfolgen.
Estimating Cepstrum of Speech Under the Presence of Noise Using a Joint Prior of Static and Dynamic Features (2004)

In this paper, we present a new algorithm for statistical speech feature enhancement in the cepstral domain. The algorithm exploits joint prior distributions (in the form of Gaussian mixture) in the clean speech model, which incorporate both the static and frame-differential dynamic cepstral parameters. Full posterior probabilities for clean speech given the noisy observation are computed using a linearized version of a nonlinear acoustic distortion model, and, based on this linear approximation, the conditional minimum mean square error (MMSE) estimator for the clean speech feature is derived rigorously using the full posterior. The final form of the derived conditional MMSE estimator is shown to be a weighted sum of three separate terms, and the sum is weighted again by the posterior for each of the mixture component in the speech model. The first of the three terms is shown to arrive naturally from the predictive mechanism embedded in the acoustic distortion model in absence of any prior information. The remaining two terms result from the speech model using only the static prior and only the dynamic prior, respectively. Comprehensive experiments are carried out using the Aurora2 database to evaluate the new algorithm. The results demonstrate significant improvement in noise-robust recognition accuracy by incorporating the joint prior for both static and dynamic parameter distributions in the speech model, compared with using only the static or dynamic prior and with using no prior.
[Study on drug food extract on lung cancer by seropharmacology]. (2005)

Seropharmacology is a useful method to the study of the effects of mixtures combined with cytobiological experiments. The inhibitive ability of HFE on lung cancer cells suggests that it has effective value in cancer prevention.
Apolipoprotein A-I possesses an anti-obesity effect associated with increase of energy expenditure and up-regulation of UCP1 in brown fat (2010)

Apolipoprotein A-I (ApoA-I) is the most abundant protein constituent of high-density lipoprotein (HDL). Reduced plasma HDL and ApoA-I levels have been found to be associated with obesity and metabolic syndrome in human beings. However, whether or not ApoA-I has a direct effect on obesity is largely unknown. Here we analysed the anti-obesity effect of ApoA-I using two mouse models, a transgenic mouse with overexpression of ApoA-I and the mice administered with an ApoA-I mimetic peptide D-4F. The mice were induced to develop obesity by feeding with high fat diet. Both ApoA-I overexpression and D-4F treatment could significantly reduce white fat mass and slightly improve insulin sensitivity in the mice. Metabolic analyses revealed that ApoA-I overexpression and D-4F treatment enhanced energy expenditure in the mice. The mRNA level of uncoupling protein (UCP)1 in brown fat tissue was elevated by ApoA-I transgenic mice. ApoA-I and D-4F treatment was able to increase UCP1 mRNA and protein levels as well as to stimulate AMP-activated protein kinase (AMPK) phosphorylation in brown adipocytes in culture. Taken together, our results reveal that ApoA-I has an anti-obesity effect in the mouse and such effect is associated with increases in energy expenditure and UCP1 expression in the brown fat tissue.
Modeling and Control of Fluid Transportation Operations in Production Plants With Petri Nets (2008)

In industrial plants various material flows take place, connecting processing and storing units to accomplish specific product routings. For example, chemical and batch plants are characterized by fluid exchange between tanks, reactors, filters, distillation columns, etc. Such fluid transportation operations may involve the use of many elementary transporting resources (e.g., pipes and valves), which can be extremely complicated to manage, especially in the case of concurrent material transfer and possibly conflicting commands. A systematic modeling and control approach for such operations is proposed based on Petri nets, which employs aggregate transportation resources denoted lines, for which suitable definitions of dependency and compatibility are provided. The actual management of valves is demanded to a specific control module, which computes valve commands with simple logical rules on the basis of the marking of resource places in the supervisor Petri net. The hierarchical and modular control architecture separates the resource allocation and deadlock avoidance problems from the physical device management, thus limiting system complexity. The general ideas discussed in this brief have been exemplified with a simple batch process example drawn from the literature.
The CCP4 suite: programs for protein crystallography (1994)

The CCP4 (Collaborative Computational Project, number 4) program suite is a collection of programs and associated data and subroutine libraries which can be used for macromolecular structure determination by X-ray crystallography. The suite is designed to be flexible, allowing users a number of methods of achieving their aims and so there may be more than one program to cover each function. The programs are written mainly in standard Fortran77. They are from a wide variety of sources but are connected by standard data file formats. The package has been ported to all the major platforms under both Unix and VMS. The suite is distributed by anonymous ftp from Daresbury Laboratory and is widely used throughout the world.
Ecallantide: A plasma kallikrein inhibitor for the treatment ofacute attacks of hereditary angioedema (2010)

Hereditary angioedema (HAE) is a debilitating, potentially fatal disease characterized by variable and unpredictable acute attacks of swelling affecting the subcutaneous tissue and mucosa. It is an autosomal dominant disorder resulting from a genetic deficiency of functional C1-esterase inhibitor. Available treatments include long-term prophylaxis, short-term prophylaxis and treatment of acute attacks. Ecallantide is a novel, specific and potent inhibitor of plasma kallikrein that was recently approved in the United States for the treatment of acute attacks of HAE in patients aged 16 years and older. In two phase III clinical trials, the subcutaneous administration of 30 mg ecallantide resulted in significantly greater symptom improvement than placebo for acute attacks of HAE. Ecallantide was generally well tolerated throughout the clinical development program. The main safety concern following ecallantide treatment is hypersensitivity reactions, including anaphylaxis. A Risk Evaluation and Management Strategy (REMS) has been implemented to minimize this risk and a long-term observational safety study is currently under way to collect more information about hypersensitivity and immunogenicity. Ecallantide represents a novel treatment option for patients with HAE.
Effect of heat-induced pain stimuli on pulse transit time and pulse wave amplitude in healthy volunteers (2015)

Pain is commonly assessed subjectively by interpretations of patient behaviour and/or reports from patients. When this is impossible the availability of a quantitative objective pain assessment tool based on objective physiological parameters would greatly benefit clinical practice and research beside the standard self-report tests. Vasoconstriction is one of the physiological responses to pain. The aim of this study was to investigate whether pulse transit time (PTT) and pulse wave amplitude (PWA) decrease in response to this vasoconstriction when caused by heat-induced pain. The PTT and PWA were measured in healthy volunteers, on both index fingers using photoplethysmography and electrocardiography. Each subject received 3 heat-induced pain stimuli using a Temperature-Sensory Analyzer thermode block to apply a controlled, increasing temperature from 32.0 °C to 50.0 °C to the skin. After reaching 50.0 °C, the thermode was immediately cooled down to 32.0 °C. The study population was divided into 2 groups with a time-interval between the stimuli 20s or 60s. The results showed a significant (p < 0.05) decrease of both PTT and PWA on the stimulated and contralateral side. Moreover, there was no significant difference between the stimulated and contralateral side. The time-interval of 20s was too short to allow PTT and PWA to return to baseline values and should exceed 40s in future studies. Heat-induced pain causes a decrease of PTT and PWA. Consequently, it is expected that, in the future, PTT and PWA may be applied as objective indicators of pain, either beside the standard self-report test, or when self-report testing is impossible.