gebhart/patrepeval huggingface dataset:
Characteristics of antitumor activity of KW-2189, a novel water-soluble derivative of duocarmycin, against murine and human tumors. (1994)

Methyl(1S)-1-bromomethyl-7-methyl-5-[(4-methylpiperazinyl)-carb onyloxy]- 3-[(5,6,7-trimethoxy-2-indolyl)-carbonyl]-1,2-dihydro-3H-pyrolo[3, 2-e] indole-8-carboxylate hydrobromide (KW-2189), a novel derivative of duocarmycin B2, was selected for extensive evaluation based on its improved antitumor activity, water solubility, and stability in the culture medium, as compared with duocarmycin B2. Although the in vitro cell growth-inhibitory activity of KW-2189 was less potent than that of duocarmycin B2, it significantly inhibited the growth of five murine solid tumors including Colon 26 adenocarcinoma, Colon 38 adenocarcinoma, and B16 melanoma in vivo. KW-2189 was also effective against murine P388 leukemia and L1210 leukemia not only by local administration (i.p.-i.p. system), but also by systemic administration (i.p.-i.v. or i.v.-i.v. system). The most remarkable feature of KW-2189 was its efficacy against various human xenografts, which was observed in 14 tumors among 16 tested tumors including drug-insensitive tumors by single i.v. administration. Tumor regression was observed in mice bearing LC-6 lung, St-4 and St-40 stomach, Li-7 liver, PAN-2 pancreas, and MX-1 breast carcinomas. In many cases, the activities of KW-2189 were more than those of clinically active agents, mitomycin C, Adriamycin, cisplatin, and cyclophosphamide. Delayed lethal toxicity, which was reported in mice treated with CC-1065 whose structure was similar to KW-2189, was not observed in mice treated with KW-2189. KW-2189 inhibited DNA synthesis more significantly than RNA or protein synthesis, although DNA strand breaks were not observed. KW-2189 was activated by porcine liver esterase, mouse liver homogenate or Hep G2 homogenate, and DU-86-DNA adducts were detected in KW-2189-treated HeLa S3 cells, suggesting that KW-2189 was converted to DU-86 in the cells. These results indicate that KW-2189 is an interesting candidate for further development as a novel antitumor agent.
Molecular characterization of group A streptococcal (GAS) oligopeptide permease (Opp) and its effect on cysteine protease production (1996)

Bacterial oligopeptide permeases are membrane-associated complexes of five proteins belonging to the ABC-transporter family, which have been found to be involved in obtaining nutrients, cell-wall metabolism, competence, and adherence to host cells. A lambda library of the strain CS101 group A streptococcal (GAS) genome was used to sequence 10,192 bp containing the five genes oppA to oppF of the GAS opp operon. The deduced amino acid sequences exhibited 50-84% homology to pneumococcal AmiA to AmiF sequences. The operon organization of the five genes was confirmed by transcriptional analysis and an additional shorter oppA transcript was detected. Insertional inactivation was used to create serotype M49 strains which did not express either the oppA gene or the ATPase genes, oppD and oppF. The mutation in oppA confirmed that the additional shorter oppA transcript originated from the opp operon and was probably due to an intra-operon transcription terminator site located downstream of oppA. While growth kinetics, binding of serum proteins, and attachment to eukaryotic cells were unaffected, the oppD/F mutants showed reduced production of the cysteine protease, SpeB, and a change in the pattern of secreted proteins. Thus, the GAS opp operon appears to contribute to both protease production and export/processing of secreted proteins.
Hematopoietic progenitors of T lymphocytes (1997)

Les lymphocytes T appartiennent a une des lignees hematopoietiques les mieux caracterisees au niveau moleculaire et cellulaire en raison de leur importance dans le systeme immunitaire. Cela contraste avec le peu d'informations dont nous disposons sur les etapes hematopoietiques precoces de production des lymphocytes T. Afin de definir le phenotype et le potentiel des progeniteurs hematopoietiques des cellules T, nous avons etudie l'activite fonctionnelle de diverses populations cellulaires exprimant la molecule de surface CD34. Ces progeniteurs CD34+ ont ete isoles a partir de moelle osseuse par cytometrie de flux et leur activite biologique a ete analysee dans un systeme de reconstitution thymique ainsi que par leur activite de reconstitution du systeme hematolymphoide que reflete la production de cellules myeloides et lymphoides. Chez l'homme comme chez la souris, il est possible d'identifier dans la moelle osseuse des progeniteurs T qui, bien qu'exprimant CD34, sont phenotypiquement et fonctionnellement distincts des cellules souches hematopoietiques. La mise en evidence de ces stades intermediaires entre cellules souches hematopoietiques et cellules T est utile pour cartographier les etapes de differenciation de cette lignee cellulaire.
Dynamic Dependencies in Application Service Management. (2000)

This paper addresses the role of dependency analysis in distributed management. It introduces the concept of dependency lifetime that traces the flow of dependency information from the design to installation to runtime stages of a service. Two categories of models, functional and structural, are used to represent this information as it flows from the design to the runtime stages. The paper describes how the dependency information at each of these stages can be retrieved and evaluated by management applications and presents an architecture to integrate these with existing network management platforms.
Homer 2 tunes G protein–coupled receptors stimulus intensity by regulating RGS proteins and PLCβ GAP activities (2003)

Homers are scaffolding proteins that bind G protein-coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels. However, their role in Ca2+ signaling in vivo is not known. Characterization of Ca2+ signaling in pancreatic acinar cells from Homer2-/- and Homer3-/- mice showed that Homer 3 has no discernible role in Ca2+ signaling in these cells. In contrast, we found that Homer 2 tunes intensity of Ca2+ signaling by GPCRs to regulate the frequency of [Ca2+]i oscillations. Thus, deletion of Homer 2 increased stimulus intensity by increasing the potency for agonists acting on various GPCRs to activate PLCbeta and evoke Ca2+ release and oscillations. This was not due to aberrant localization of IP3Rs in cellular microdomains or IP3R channel activity. Rather, deletion of Homer 2 reduced the effectiveness of exogenous regulators of G proteins signaling proteins (RGS) to inhibit Ca2+ signaling in vivo. Moreover, Homer 2 preferentially bound to PLCbeta in pancreatic acini and brain extracts and stimulated GAP activity of RGS4 and of PLCbeta in an in vitro reconstitution system, with minimal effect on PLCbeta-mediated PIP2 hydrolysis. These findings describe a novel, unexpected function of Homer proteins, demonstrate that RGS proteins and PLCbeta GAP activities are regulated functions, and provide a molecular mechanism for tuning signal intensity generated by GPCRs and, thus, the characteristics of [Ca2+]i oscillations.
ChemInform Abstract: Bio‐Supported Palladium Nanoparticles as a Phosphine‐Free Catalyst for the Suzuki Reaction in Water. (2012)

Abstract Palladium nanoparticles, immobilized on bacterial cellulose, are successfully applied in the cross‐coupling of aryl iodides and chlorides with arylboronic acids [cf.
Generation of Functional Murine Cardiac Myocytes From Induced Pluripotent Stem Cells (2008)

iPS cells differentiate into functional cardiomyocytes. In contrast to ES cells, iPS cells allow derivation of autologous functional cardiomyocytes for cellular cardiomyoplasty and myocardial tissue engineering.
Neisseria gonorrhoeaeandChlamydia trachomatisinfection in HIV-1-infected women taking antiretroviral therapy: a prospective cohort study from Burkina Faso: Table 1 (2013)

The risk of NG or CT infection remains low among high-risk women in Bobo-Dioulasso. This provides some evidence that antiretroviral use does not contribute to behavioural disinhibition. The asymptomatic nature of most infections underscores the need for regular screening and treatment of STIs in core groups.
A Facile Ligation Approach to Prepare Three-Helix Bundles of HIV Fusion-State Protein Mimetics (2002)

We have designed a facile ligation approach to prepare three-helix bundles mimicking the HIV membrane fusion-state proteins that may be useful as inhibitors and vaccine candidates for blocking HIV infection. [reaction: see text]
Abbott AxSYM random and continuous access immunoassay system for improved workflow in the clinical laboratory (1993)

We describe a new clinical laboratory instrument, the Abbott AxSYM, which provides random- and continuous-access testing for immunoassays, 20 onboard reagents, primary tube sampling, and a throughput of 80 to 120 tests per hour. The AxSYM incorporates three separate analytical technologies for processing immunoassays: microparticle enzyme immunoassay, fluorescence polarization immunoassay, and a novel technology known as ion-capture immunoassay. The system incorporates both common and technology-specific subsystems controlled by a real-time software scheduling processor. Tests can be processed in one- or two-step sandwich or competitive formats, with variable pipetting steps, incubation periods, optical read formats, and wash sequences. Menu capabilities include tests for hepatitis, retrovirus, tumor markers, fertility markers, thyroid functions, and therapeutic drugs. The time to first result is approximately 15-25 min for most routine assays and < or = 15 min for stat assays (i.e., creatine kinase MB isoenzyme, human chorionic gonadotropin beta subunit, and therapeutic drugs). AxSYM assay performance for 23 assays was comparable with that of the Abbott IMx and TDx analyzers; specimen correlation data had correlation coefficients ranging from 0.97 to 0.99 and slopes ranging from 0.99 to 1.10. Within-run imprecision (CV) was 1.5% to 11.4%, with most assays (19 of 23) demonstrating CVs < or = 8.0%.
The Amino Terminus of the Saccharomyces cerevisiae DNA Helicase Rrm3p Modulates Protein Function Altering Replication and Checkpoint Activity (2004)

The Pif1 family of DNA helicases is conserved from yeast to humans. Although the helicase domains of family members are well conserved, the amino termini of these proteins are not. The Saccharomyces cerevisiae genome encodes two Pif1 family members, Rrm3p and Pif1p, that have very different functions. To determine if the amino terminus of Rrm3p contributes to its role in promoting fork progression at >1000 discrete chromosomal sites, we constructed a deletion series that lacked portions of the 249-amino-acid amino terminus. The phenotypes of cells expressing alleles that lacked all or most of the amino terminus were indistinguishable from those of rrm3Delta cells. Rrm3p deletion derivatives that lacked smaller portions of the amino terminus were also defective, but the extent of replication pausing at tRNA genes, telomeres, and ribosomal DNA (rDNA) was not as great as in rrm3Delta cells. Deleting only 62 amino acids from the middle of the amino terminus affected only rDNA replication, suggesting that the amino terminus can confer locus-specific effects. Cells expressing a fusion protein consisting of the Rrm3p amino terminus and the Pif1p helicase domain displayed defects similar to rrm3Delta cells. These data demonstrate that the amino terminus of Rrm3p is essential for Rrm3p function. However, the helicase domain of Rrm3p also contributes to its functional specificity.
Total Oxidant Scavenging Capacity of Euterpe oleracea Mart. (Açaí) Seeds and Identification of Their Polyphenolic Compounds (2006)

The antioxidant capacity of methanol and ethanol seed extracts from Euterpe oleracea Mart. (açaí) against the reactive oxygen species (ROS) peroxyl radicals, peroxynitrite, and hydroxyl radicals was studied with the total oxidant scavenging capacity (TOSC) assay in a modified and automated version. Cold methanol digestion was the most efficient extraction method with respect to the antioxidant capacity. The extracts exhibit good antioxidant capacity against peroxyl radicals, similar to the capacity of the pulp. The antioxidant capacity against peroxynitrite and hydroxyl radicals is even higher. The main antioxidants identified by HPLC-MS and HPLC-CEAD are five different procyanidins (di- through pentamers); furthermore, protocatechuic acid and epicatechin were identified as minor compounds. Determination of TOSC values of HPLC seed extract fractions indicates that the procyanidins contribute substantially to the overall antioxidant capacity. In addition, however, other compounds that have not yet been identified are responsible for a large part of the observed antioxidant capacity.
Hydroxylaminolysis of penicillin binding componenets is enzymatically catalyzed. (1977)

The hydroxylaminolysis of the penicilloyl moiety from [14C]penicillin G binding component (PBC) complexes of the Bacillus subtilis D-alanine carboxypeptidase and of the mixture of PBC's of Staphylococcus aureus was inhibited by denaturation of the complexes by heat (55 degrees), detergent (1% sodium dodecyl sulfate), or trichloroacetic acid. The kinetics of inhibition by denaturation were comparable to those of the inhibition of [14C]penicillin G binding to the PBC's and of carboxypeptidase activity of the B. subtilis enzyme under identical denaturing conditions. These data establish that the hydroxylaminolysis is an enzymatically catalyzed process suggesting that penicillin G is bound to an enzymatically active site. Treatment of the denatured [14C]penicillin G-carboxypeptidase complex with sodium borohydride or at pH 12 resulted in the release of the penicilloyl moiety. These results are consistent with a carboxylic ester bond for the penicilloyl-PBC instead of a thiolester linkage as was initially presumed.
Expression of the Anabaena hetR gene from a copper-regulated promoter leads to heterocyst differentiation under repressing conditions (2001)

Heterocyst differentiation in the filamentous cyanobacterium Anabaena PCC 7120 requires a functional hetR gene. Increased expression of the hetR gene is seen in developing and mature heterocysts in response to fixed nitrogen limitation. We mapped four likely transcriptional start sites for hetR and identified a specific transcript that is positively autoregulated. By using the copper-responsive petE promoter from Anabaena PCC 7120 to drive hetR expression, we show that ectopic expression of hetR increases heterocyst frequency and induces heterocyst differentiation under fully repressing conditions. Coexpression of a reporter gene shows that expression from the petE promoter is smoothly induced depending on the amount of copper supplied. In the heterocyst pattern mutant PatA, where terminally positioned heterocysts are formed almost exclusively, expression of the petEhetR fusion does not result in the formation of intercalary heterocysts. These results suggest that although the intracellular concentration of HetR has to be elevated for the differentiation decision, PatA plays a role as well. This role may be in the form of posttranslational modification of HetR, because PatA is a member of the response regulator family of proteins.
Demonstration Of The Caustic-side Solvent Extraction Process For The Removal Of137Cs From Savannah River Site High Level Waste (2003)

Abstract This article describes a demonstration of a solvent extraction process for removal of 137Cs from alkaline high-level radioactive waste (HLW) solutions from the Savannah River Site (SRS) tank farm. The process employed a calix[4]arene-crown-6 extractant dissolved in an inert hydrocarbon matrix, along with an alkylphenoxy alcohol modifier and an additional additive, trioctylamine, to improve stripping performance and mitigate the effects of organic anions present in the feed stream. Testing included washing and recycle of the solvent. Tests conducted with two nonradioactive simulants of SRS tank waste verified proper hydraulic operation prior to the radioactive experiments. An additional 12-hour demonstration used simulated SRS tank waste spiked with 137Cs to confirm proper hydraulic operation, decontamination, and volume reduction factors following installation of the equipment in a shielded cell facility. Finally, a 48-hour demonstration was performed employing a composite waste from SRS tank farms. Approximately 106 L of waste (1.5E+09 d/min/mL 137Cs activity) was processed through the test unit. Average 137Cs decontamination factors for the decontaminated waste streams in all tests exceeded the target value of 40,000. Concentration factors in the strip section agreed to within 5% of the target value of 15. #The submitted manuscript has been authored by a contractor of the U.S. Government under contract No. DE-AC09-96SR18500. Accordingly, the U.S. Government retains a non-exclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes. Keywords: CesiumWaste processingSolvent extractionCentrifugal contactors Acknowledgments Notes #The submitted manuscript has been authored by a contractor of the U.S. Government under contract No. DE-AC09-96SR18500. Accordingly, the U.S. Government retains a non-exclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes.
Strong polarization toward Th1 immune response in ANCA-associated glomerulonephritis (2003)

Both peripheral and renal immune responses are strongly polarized toward Th1 type immune response in ANCA-associated GN. Peripheral Th1/Th2 ratio may reflect the immune responses in renal injury of ANCA-associated GN.
La categoría paradigma en la investigación social (2005)

CONTENIDO\n\n\n\n\n\nArtículos\n\n\n\nLa categoría paradigma en la investigación social \nToro, Abraham y Marcano, Luzmila\n\n\n\nEl concepto de la historia revisitado desde Mario Briceño Iragorry\nHernández C., Luis Javier\n\n\n\nClero regular y sociedad canaria en el antiguo régimen: los conventos de la Orotava\nHernández G., Manuel\n\n\n\nLa educación institucional en la jurisdicción Cabudareña 1832-1908 (actuales Municipios Simón Planas y Palavecino) estado Lara - Venezuela\nAris, Yolanda\n\n\n\n\nBioética, cultura y responsabilidad social\nMora García, José Pascual\n\n\n\n\n\nConversaciones\n\n\n\nConversando con La Sociedad Venezolana de Historia de la Educación (SVHE)\nMora García, José Pascual\n\n\n\n\n\nReseñas\n\n\n\n\nMuseo Pedagógico "Temístocles Salazar". Servicios Bibliotecarios Núcleo Táchira\nReseñado por: Mora García, José Pascua\n\n\n\n\nEventos\n\n\n\n\nI Congreso Internacional de Ciencias Historicas y XI Jornada Nacional sobre Investigacion y Docencia en la Ciencia de la Historia, Barquisimeto, 27 de Julio de 2005
Fluorescence in situ hybridization analysis of c‐myc amplification in stage T3N0M0 prostate cancer in Japanese patients (2006)

Identification of an AI-c-myc may serve as a potential marker of prostate cancer progression.
A MEMS Probe Card with High Aspect Ratio Electroplated Posts (2007)

In this paper, a MEMS probe card developed with high aspect ratio post in order to implement high speed and high frequency testing and reduce thermal deformation effect during hot test of device under test (DUT) is discussed. High aspect ratio posts were fabricated by nickel electroplating after silicon etching and oxidation performed by a deep RIE etcher and furnace, respectively. Probe card makes contact with the metal pads of the DUT, thereby transmitting the test signals to the DUT. According to reliability test, there was no mechanical bending to cantilever beam and position difference to tips after the 350,000 time touchdowns. The proposed probe card is suitable for wafer-level testing and fine pitch device testing.
Relationship of microglial and astrocytic activation to disease onset and progression in a transgenic model of familial ALS (1998)

Transgenic mice that highly over-express a mutated human CuZn superoxide dismutase (SOD1) gene [gly93-->ala; TgN(SOD1-G93A)G1H line] found in some patients with familial ALS (FALS) have been shown to develop motor neuron disease that is characterized by motor neuron loss in the lumbar and cervical spinal regions and a progressive loss of motor activity. The mutant Cu,Zn SOD exhibits essentially normal SOD activity but also generates toxic oxygen radicals as a result of an enhancement of a normally minor peroxidase reaction. Consequently, lipid and protein oxidative damage to the spinal motor neurons occurs and is associated with disease onset and progression. In the present study, we investigated the time course of microglial (major histocompatibility-II antigen immunoreactivity) and astrocytic (glial fibrillary acidic protein immunoreactivity) activation in relation to the course of motor neuron disease in the TgN(SOD1-G93A)G1H FALS mice. Four ages were investigated: 30 days (pre-motor neuron pathology and clinical disease); 60 days (after initiation of pathology, but pre-disease); 100 days (approximately 50% loss of motor neurons and function); and 120 days (near complete hindlimb paralysis). Compared to non-transgenic littermates, the TgN(SOD1-G93A)G1H mice showed significantly increased numbers of activated astrocytes (P < 0.01) at 100 days of age in both the cervical and lumbar spinal cord regions. However, at 120 days of age, the activation lost statistical significance. In contrast, microglial activation was significantly increased several-fold at both 100 and 120 days. We hypothesize that astrocytic activation may exert a trophic influence on the motor neurons that is insufficiently maintained late in the course of the disease. On the other hand, the sustained, intense microglial activation may conceivably contribute to the oxidative stress and damage involved in the disease process. If true, then agents which inhibit microglia may help to limit disease progression.
In vitro synthesis of oncogenic human papillomaviruses requires episomal genomes for differentiation-dependent late expression. (1996)

Human papillomavirus (HPV) types 16, 18, 31, and 51 are the etiologic agents of many anogenital cancers including those of the cervix. These "high risk" HPVs specifically target genital squamous epithelia, and their lytic life cycle is closely linked to epithelial differentiation. We have developed a genetic assay for HPV functions during pathogenesis using recircularized cloned HPV 31 genomes that were transfected together with a drug resistance marker into monolayer cultures of normal human foreskin keratinocytes, the natural host cell. After drug selection, cell lines were isolated that stably maintained HPV 31 DNA as episomes and underwent terminal differentiation when grown in organotypic raft cultures. In differentiated rafts, the expression of late viral genes, amplification of viral DNA, and production of viral particles were detected in suprabasal cells. This demonstrated the ability to synthesize HPV 31 virions from transfected DNA templates and allowed an examination of HPV functions during the vegetative viral life cycle. We then used this system to investigate whether an episomal genome was required for the induction of late viral gene expression. When an HPV 31 genome (31E1*) containing a missense mutation in the E1 open reading frame was transfected into normal human keratinocytes, the mutant viral sequences were found to integrate into the host cell chromosomal DNA with both early and late regions intact. While high levels of early viral gene transcription were observed, no late gene expression was detected in rafts of cell lines containing the mutant viral genome despite evidence of terminal differentiation. Therefore, the induction of late viral gene expression required that the viral genomes be maintained as extrachromosomal elements, and terminal differentiation alone was not sufficient. These studies provide the basis for a detailed examination of HPV functions during viral pathogenesis.