gebhart/patrepeval huggingface dataset:
Communication architecture and protocols for broadcast-type mobile multimedia ad hoc networks (2003)

Communication architecture and related protocols are proposed for broadcast-type mobile multimedia ad hoc networks. Communication among nodes is achieved through two distinct layers that are characterized by clustering of nodes and separation of communication channels. Nodes are grouped in a disjoint manner into clusters, each cluster consisting of one or more members. One node that is one hop away from the other members in the cluster is designated as a leader or clusterhead; it acts as a communication portal for members to reach out to other nodes within or outside their clusters. We propose a cluster assisted routing (CAR) protocol that reduces the copies of route discovery messages significantly by utilizing cluster information. One distinct channel is dedicated to inter-cluster communication and the other to intra-cluster communication. Communication overhead is balanced between these two channels since the clusterhead handles more packets while the number of clusterheads is much less than that of members. This model also improves the cost of path discovery and maintenance as well as expected data delivery time.
Food-Evoked Changes in Humans (2010)

Two experiments investigate effects related to food intake in humans. In Experiment 1, we measured startle response modulation while study participants ate ice cream, yoghurt, and chocolate. Statistical analysis revealed that ice cream intake resulted in the most robust startle inhibition compared to no food. Contrasting females and males, we found significant differences related to the conditions yoghurt and chocolate. In females, chocolate elicited the lowest response amplitude followed by yoghurt and ice cream. In males, chocolate produced the highest startle response amplitude even higher than eating nothing, whereas ice cream produced the lowest. Assuming that high response amplitudes reflect aversive motivation while low response amplitudes reflect appetitive motivational states, it is interpreted that eating ice cream is associated with the most appetitive state given the alternatives of chocolate and yoghurt across gender. However, in females alone eating chocolate, and in males alone eating ice cream, led to the most appetitive state. Experiment 2 was conducted to describe food intake-related brain activity by means of source localization analysis applied to electroencephalography data (EEG). Ice cream, yoghurt, a soft drink, and water were compared. Brain activity in rostral portions of the superior frontal gyrus was found in all conditions. No localization differences between conditions occurred. While EEG was found to be insensitive, startle response modulation seems to be a reliable method to objectively quantify motivational states related to the intake of different foods.
ACAPELLA-1K, a capillary-based submicroliter automated fluid handling system for genome analysis. (2000)

The Genomation Laboratory in the Electrical Engineering Department at the University of Washington has been developing an automated, high-throughput, submicroliter-scale fluid-handling system for use in molecular biology, especially as part of the Human Genome Project and other high-throughput DNA sequencing endeavors. Small glass capillaries enable the preparation, handling, and monitoring of 1-microliter reaction volumes. The Genomation Laboratory, with corporate partners Orca Photonic Systems, Inc. and Engineering Arts, has developed modules for aspiration, dispensing, mixing, transport, and rapid thermal processing of biological samples contained in glass capillaries. The ACAPELLA-1K is the first integration of these modules, designed to process 1000 samples in an eight-hour day. It has served as a test bed for the technologies as well as for performing biological experiments in conjunction with the University of Washington Genome Center. This system and related results are presented in this paper. A video of the system in operation is provided at. The Genomation Laboratory is presently developing the next-stage ACAPELLA-5K system based on the results of the ACAPELLA-1K system.
Using a quantum computer to investigate quantum chaos (1998)

We show that the quantum baker's map, a prototypical map invented for theoretical studies of quantum chaos, has an efficient realization in terms of quantum gates. Chaos in the quantum baker's map could be investigated experimentally on a quantum computer based on only three quantum bits.
Reversal of nzb/nzw disease with total lymphoid irradiation. (1979)

NZB/NZW mice spontaneously exhibit autoimmune disease similar to that seen in human systemic lupus erythematosus (SLE). We demonstrated that total lymphoid irradiation (TLI) reversed well expressed disease in 6-mo-old NZB/NZW females with a prolongation in survival, decrease in proteinuria, and decrease in anti-DNA antibodies as compared to control animals. Few side effects were observed in the treated group. TLI also prolonged survival in animals with advanced renal disease. These findings suggest that TLI may have application to the treatment of human SLE.
The Linking Regions of EBNA1 Are Essential for Its Support of Replication and Transcription (1999)

The ability of distant cis-acting DNA elements to interact functionally has been proposed to be mediated by the interaction of proteins associated site specifically with those cis-acting elements. We have found that the DNA-linking regions of EBNA1 are essential for its contribution to both replication and transcription. The synthesis of plasmids containing the Epstein-Barr virus (EBV) origin of plasmid replication (oriP) can be mediated entirely by the cellular machinery; however, the replicated molecules are lost rapidly from proliferating cells. When EBNA1 is provided in trans, plasmids containing oriP (oriP plasmids) are synthesized during repeated S phases, and the newly formed daughter molecules are precisely segregated to the daughter cells. The contribution(s) of EBNA1 to the stable replication of oriP plasmids is therefore likely to be postsynthetic. In latently infected cells, EBNA1 also regulates the expression of multiple EBV promoters located as many as 10 kbp away. EBNA1 supports replication and transcription through binding to oriP; both the ability of EBNA1 to bind to DNA and the integrity of its binding sites in oriP are required. However, DNA binding by EBNA1 is not sufficient to support replication or transcription, indicating that an additional activity (or activities) is required. EBNA1 links DNAs to which it binds and can form a loop between the two subelements of oriP, the family of repeats and the region of dyad symmetry, each of which contains multiple binding sites for EBNA1. We have constructed a set of derivatives of EBNA1 which contain both, one, or neither of its linking regions in various contexts. Analyses of these derivatives demonstrate that the linking regions of EBNA1 are essential for its support of replication and transcription and that the ability of derivatives of EBNA1 to link DNAs correlates strongly with their support of these activities in cells. These findings indicate that protein-protein associations of the linking regions of EBNA1 underlie its long-range contributions to replication and transcription.
Microelectrodes, Microelectronics, and Implantable Neural Microsystems (2008)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> Lithographically defined microelectrode arrays now permit high-density recording and stimulation in the brain and are facilitating new insights into the organization and function of the central nervous system. They will soon allow more detailed mapping of neural structures than has ever before been possible, and capabilities for highly localized drug-delivery are being added for treating disorders such as severe epilepsy. For chronic neuroscience and neuroprosthesis applications, the arrays are being used in implantable microsystems that provide embedded signal processing and wireless data transmission to the outside world. A 64-channel microsystem amplifies the neural signals by 60 dB with a user-programmable bandwidth and an input-referred noise level of 8 <formula formulatype="inline"><tex>$\mu{\hbox{V}}_{\rm rms}$</tex></formula> before processing the signals digitally. The channels can be scanned at a rate of 62.5 kS/s, and signals above a user-specified biphasic threshold are transmitted wirelessly to the external world at 2 Mbps. Individual channels can also be digitized and viewed externally at high resolution to examine spike waveforms. The microsystem dissipates 14.14 mW from 1.8 V and measures 1.4 <formula formulatype="inline"><tex>$\times$</tex></formula> 1.55 cm<formula formulatype="inline"> <tex> $^2$</tex></formula>. </para>
Quality estimation for Machine Translation output using linguistic analysis and decoding features (2012)

We describe a submission to the WMT12 Quality Estimation task, including an extensive Machine Learning experimentation. Data were augmented with features from linguistic analysis and statistical features from the SMT search graph. Several Feature Selection algorithms were employed. The Quality Estimation problem was addressed both as a regression task and as a discretised classification task, but the latter did not generalise well on the unseen testset. The most successful regression methods had an RMSE of 0.86 and were trained with a feature set given by Correlation-based Feature Selection. Indications that RMSE is not always sufficient for measuring performance were observed. 1
Immunization of monkeys with varicella‐zoster virus glycoprotein antigens and their response to challenge with simian varicella virus (1987)

African green monkeys (Cercopithecus aethiops) were immunized with three intramuscular injections of gpI, gpII, or gpIII glycoprotein antigens of varicella-zoster virus (VZV). Antibody responses to VZV were determined by enzyme-linked immunosorbent assay (ELISA) and to simian varicella virus (SVV) by immunofluorescence and by serum neutralization assays. Two weeks following the third immunization with VZV glycoproteins, the monkeys were challenged by inoculation of SVV. Antibodies to gpII or gpIII partially prevented infection by SVV, while the presence of antibodies to gpI was ineffective in preventing disease induced by SVV challenge. Factors affecting the immunogenicity of these antigens in this model are discussed.
In vivo breast cancer characterization imaging using two monoclonal antibodies activatably labeled with near infrared fluorophores (2012)

Multi-color target-specific fluorescence breast cancer imaging can be achieved in vivo by employing two activatable fluorescent probes administered as a cocktail. The images allowed us to see a specific receptor expression in each breast tumor without post-image processing.
A simple and sensitive procedure for measuring isotope fluxes through ion-specific channels in heterogenous populations of membrane vesicles. (1983)

In this paper, we describe a simple and highly sensitive manual assay for isotope fluxes through ion-conducting pathways, particularly cation-specific channels, in heterogenous populations of small membrane vesicles. We measure uptake of tracer of the ion of interest, against a large chemical gradient of the same ion. As a result of the imposed chemical gradient, a transient electrical diffusion potential is set up across the membranes of those vesicles which are highly permeable to the ion of interest. The isotope tends to equilibrate with the diffusion potential and is therefore concentrated selectively and transiently into those vesicle containing the channels. Furthermore, when performed in this way, the time course of tracer equilibration occurs over several minutes, rather than the sub-second range expected for tracer equilibration into channel-containing vesicles in the absence of an opposing chemical gradient of the permeant ion. The use of the procedure is demonstrated for three Na-conducting channels: gramicidin D incorporated into phospholipid vesicles, amiloride-blockable Na channels in toad bladder microsomes, and veratridine-activated tetrodotoxin-blockable Na channels in rat brain synaptic membranes. For all three cases, it proved simple to measure a specific 22Na uptake, in a minute time range, using very low concentrations of the channel-containing vesicles. By comparison with isotope flux measurements performed without an opposing Na gradient, the power of the present assay derives from both the very large gain in sensitivity and the convenient time course.
Preferential synthesis of heptaacyl lipopolysaccharide by the ssc permeability mutant of Salmonella typhimurium (1992)

In Salmonella typhimurium, a chromosomal gene termed ssc has been shown to cause an antibiotic-supersensitive phenotype. We studied the effect of the ssc gene on the chemical composition of the lipopolysaccharide component, using a thermosensitive ssc1 mutant (SH7622) that grows poorly at 42 degrees C. Analysis of the lipopolysaccharide by various techniques including fast-atom-bombardment mass spectrometry of lipid A, and determination of the type of linkage of fatty acids, revealed a profound temperature-dependent effect associated with the ssc1 mutation. At the non-permissive temperature, SH7622 contained hexadecanoic acid in the majority of lipid A molecules, resulting in the exclusive presence of heptaacyl lipopolysaccharide. This effect was largely reversed by the introduction of the cloned wild-type ssc gene to SH7622 and much reduced by growth of SH7622 at 37 degrees C.
Depiction of Seniors in Packaged Food Products Commercials (1996)

This manuscript sets forth thc results of an examination of the portrayal of older persons in television commercials sponsored by packaged food manufacturers. The analysis indicated that the advertisements featured older persons less frequently and less positively than was the case for younger consumers. The manuscript sets forth some implications for packaged food products marketers, as they relate to depicting seniors and depicting them positively.
Metal‐Ion Metathesis in Metal–Organic Frameworks: A Synthetic Route to New Metal–Organic Frameworks (2012)

A porous metal-organic framework, Mn(H(3)O)[(Mn(4)Cl)(3)(hmtt)(8)] (POST-65), was prepared by the reaction of 5,5',10,10',15,15'-hexamethyltruxene-2,7,12-tricarboxylic acid (H(3)hmtt) with MnCl(2) under solvothermal conditions. POST-65(Mn) was subjected to post-synthetic modification with Fe, Co, Ni, and Cu according to an ion-exchange method that resulted in the formation of three isomorphous frameworks, POST-65(Co/Ni/Cu), as well as a new framework, POST-65(Fe). The ion-exchanged samples could not be prepared by regular solvothermal reactions. The complete exchange of the metal ions and retention of the framework structure were verified by inductively coupled plasma-atomic emission spectrometry (ICP-AES), powder X-ray diffraction (PXRD), and Brunauer-Emmett-Teller (BET) surface-area analysis. Single-crystal X-ray diffractions studies revealed a single-crystal-to-single-crystal (SCSC)-transformation nature of the ion-exchange process. Hydrogen-sorption and magnetization measurements showed metal-specific properties of POST-65.
Tumor-Bound Immunoglobulin in Human Gynecologic Cancers<xref ref-type="fn" rid="FN2">2</xref> (1979)

By the use of immunofluorescence techniques, immunoglobulin of the IgG class was consistently found in touch preparations and in frozen sections of squamous cell carcinomas of the uterine cervix (both keratinizing and nonkeratinizing) and in an adenosquamous cell carcinoma of the cervix, an adenocarcinoma of the cervix, a mixed mesodermal-uterine tumor, and a uterine adenocarcinoma metastasized to the ovaries. Trace amountsof IgM were found in 1 squamous cell carcinoma and in 1 adenocarcinoma of the cervix. Except for 1 tumor specimen consisting primarily of infiltrating lymphocytes that stained positive for human IgG, IgM, IgA, and C3, the tumors were consistently negative for IgA and C3. Specimens made from normal cervical tissue were uniformly negative for all immunoglobulins and complement. Positive staining for human IgG could be eliminated by incubation of the tumor preparations with unconjugated goat antihuman IgG before the preparations were stained with fluorescein isothiocyanate-conjugated goat antihuman IgG. Attempts to elute the tumor-bound IgG with glycine-HCl buffer (pH 2.2) were most successful with the use of unfixed tissue, although the positive staining for IgG could not be entirely eliminated. The elution effects of the low-pH buffer on tissue fixed over 2 hours in 10% Formalin were minimal.
Nitroglycerin Improves the Hemodynamic Response to Vasopressin in Portal Hypertension (1982)

This study was designed to investigate whether the addition of nitroglycerin to vasopressin infusion could avoid the deleterious systemic effects of vasopressin while maintaining or enhancing the therapeutic benefits of portal pressure reduction. The effect of nitroglycerin on splanchnic and systemic hemodynamics was studied in cirrhotic patients and portal hypertensive dogs receiving i.v. vasopressin. During i.v vasopressin infusion (0.4 units per min), the cardiac output decreased in patients by 14% from 7.6 +/- 0.9 (mean +/- S.E.) to 6.5 +/- 0.7 liters per min, p less than 0.01, the mean arterial pressure increased 21% from 87 +/- 2 to 105 +/- 4, p less than 0.01, and the heart rate decreased 11% from 79 +/- 3 to 71 +/- 3, p less than 0.01. The administration of sublingual nitroglycerin (0.4 mg) returned all the systemic hemodynamic parameters to baseline values. In dogs, vasopressin infusion significantly reduced portal pressure and flow while increasing portal venous resistance. Nitroglycerin when added to the vasopressin infusion reduced portal venous resistance and further decreased portal pressure in dogs. In patients, vasopressin reduced the hepatic blood flow (44%), wedged hepatic venous pressure (11%), and the gradient between wedged and free hepatic venous pressures (23%). Nitroglycerin administration caused a further reduction of the wedged hepatic venous pressure (23.6 +/- 2.3 to 21.1 +/- 2.0, 11%, p less than 0.01). There was a small but not significant further decline (7%) in the hepatic venous pressure gradient. These results provide evidence that the addition of nitroglycerin to an i.v. infusion of vasopressin reversed the detrimental effects of vasopressin while preserving the beneficial effects.
Network Address Translation - Protocol Translation (NAT-PT) (2000)

This document specifies an IPv4-to-IPv6 transition mechanism, in addition to those already specified in [TRANS].This solution attempts to provide transparent routing, as defined in [NAT-TERM], to end-nodes in V6 realm trying to communicate with end-nodes in V4 realm and vice versa.This is achieved using a combination of Network Address Translation and Protocol Translation.The scheme described does not mandate dual-stacks (i.e., IPv4 as well as V6 protocol support) or special purpose routing requirements (such as requiring tunneling support) on end nodes.This scheme is based on a combination of address translation theme as described in [NAT-TERM] and V6/V4 protocol translation theme as described in [SIIT].
A rapid method for the isolation of functional thymus‐derived murine lymphocytes (1973)

Abstract A rapid method is described for effectively removing immunoglobulin‐bearing cells from either primed or unprimed mouse spleen and lymph node cell suspensions. Incubation of cell suspensions in nylon wool columns for 45 min at 37 °C resulted in a 9 to 100‐fold depletion of immunoglobulin‐bearing cells and a complementary 1.5 to 2‐fold enrichment of T cells in the column effluent populations. The effluent population, derived from passage of spleen cells through these columns, was virtually devoid of B precursor and memory cell activity, but contained all of the helper cell and cytotoxic effector cell precursor activity when compared to unfractionated spleen cells.
Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance (1993)

Several species of the genus Fusarium and related fungi produce trichothecenes which are sesquiterpenoid epoxides that act as potent inhibitors of eukaryotic protein synthesis. Interest in the trichothecenes is due primarily to their widespread contamination of agricultural commodities and their adverse effects on human and animal health. In this review, we describe the trichothecene biosynthetic pathway in Fusarium species and discuss genetic evidence that several trichothecene biosynthetic genes are organized in a gene cluster. Trichothecenes are highly toxic to a wide range of eukaryotes, but their specific function, if any, in the survival of the fungi that produce them is not obvious. Trichothecene gene disruption experiments indicate that production of trichothecenes can enhance the severity of disease caused by Fusarium species on some plant hosts. Understanding the regulation and function of trichothecene biosynthesis may aid in development of new strategies for controlling their production in food and feed products.
Retention of Coagulation Factors and Storage of Freeze-Dried Plasma (2021)

The TFDP process had no negative impact on coagulation factor activity. Input plasma and anticoagulant type did not affect TFDP quality. Pooling FFP normalized factor variability in TFDP and did not negatively impact product quality. The TFDP is stable for up to 24 months at room and refrigerated temperatures. Terumo's freeze-dried plasma is comparable to PF24/FFP. It does not require complex logistics or time-consuming thawing. Terumo's freeze-dried plasma may be suitable for rapid treatment of coagulopathies with logistical advantages over PF24/FFP.
Gene Expression Dynamics Inspector (GEDI): for integrative analysis of expression profiles (2003)

http://www.chip.org/~ge/gedihome.html
A novel trifunctional IgG-like bispecific antibody to inhibit HIV-1 infection and enhance lysis of HIV by targeting activation of complement (2010)

The targeted complement activator, (anti-gp120 x anti-C3d)-Fc, can be used as a novel approach to HIV therapy by abrogating the complement-enhanced HIV infection of cells.
Facile labelling of an anti-epidermal growth factor receptor Nanobody with 68Ga via a novel bifunctional desferal chelate for immuno-PET (2011)

Via a rapid procedure under mild conditions a (68)Ga-Nanobody was obtained that exhibited high tumour uptake and tumour to normal tissue ratios in nude mice bearing A431 xenografts. Fast kinetic (68)Ga-Nanobody conjugates can be promising tools for tumour detection and imaging of target expression.
Automated Peritoneal Dialysis: New Implications for Pharmacists (1997)

Well-designed studies are needed to fully characterize the pharmacokinetic parameters of drugs in APD. Until then, pharmacists should recommend that intraperitoneally administered drugs be given during the longest peritoneal dialysate dwell of the day and that serum concentrations of drugs with narrow therapeutic indices be monitored closely.