gebhart/patrepeval huggingface dataset:
Elevated protein kinase C betaII is an early promotive event in colon carcinogenesis. (2001)

Protein kinase C (PKC) has been implicated in colon carcinogenesis in humans and in rodent models. However, little is known about the specific role of individual PKC isozymes in this process. We recently demonstrated that elevated expression of PKC betaII in the colonic epithelium induces hyperproliferation in vivo (N. R. Murray et al., J. Cell Biol., 145: 699-711, 1999). Because hyperproliferation is a major risk factor for colon cancer, we assessed whether specific alterations in PKC betaII expression occur during azoxymethane-induced colon carcinogenesis in mice. An increase in PKC betaII expression was observed in preneoplastic lesions (aberrant crypt foci, 3.7-fold) compared with saline-treated animals, and in colon tumors (7.8-fold; P = 0.011) compared with uninvolved colonic epithelium. In contrast, PKC alpha and PKC betaI (a splicing variant of PKC betaII) expression was slightly decreased in aberrant crypt foci and dramatically reduced in colon tumors. Quantitative reverse transcription-PCR analysis revealed that PKC mRNA levels do not directly correlate with PKC protein levels, indicating that PKC isozyme expression is likely regulated at the posttranscriptional/translational level. Finally, transgenic mice expressing elevated PKC betaII in the colonic epithelium exhibit a trend toward increased colon tumor formation after exposure to azoxymethane. Taken together, our results demonstrate that elevated expression of PKC betaII is an important early, promotive event that plays a role in colon cancer development.
Object and native code thread mobility among heterogeneous computers (includes sources) (1995)

We present a technique for moving objects and threads among heterogeneous computers at the native code level. To enable mobility of threads running native code, we convert thread states among machine-dependent and machine-independent formats. We introduce the concept of bLts stops, which are machine-independent representations of program points as represented by program counter values, The concept of bus stops can be used also for other purposes, <~. g., to aid inspecting and debugging optimized code, garbage collection e~c. We also discuss techniques for thread mobility among processors executing differently optimized codes.
The effect of SWCNT and nano‐diamond films on human osteoblast cells (2007)

Abstract Human osteoblasts (SAOS‐2) are cultured on single‐walled carbon nanotube (SWCNT) and nanocrystalline diamond (NCD) films in order to evaluate their potential for biological application. We present dependence of metabolic activity and morphology of osteoblasts on (i) material substrate (NCD or SWCNT films), (ii) surface termination – O‐termination causing hydrophilic surface and H‐termination (as deposited NCDs) causing hydrophobic surface, (iii) surface roughness in the range of 11–39 nm RMS and (iv) SWCNT production technique – laser ablation, arc‐discharge and HiPco. The metabolic activity decreases to the same non‐toxic level on SWCNT films produced by different techniques as well as on H‐terminated NCD compared to tissue culture polystyrene. The metabolic activity is increasing on O‐terminated NCD films as a function of their roughness. Morphological observations support superior properties of O‐terminated NCD films. (© 2007 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)
Quantitative Characterization of a Hardwood Milled Wood Lignin by Nuclear Magnetic Resonance Spectroscopy (2005)

The structure of Eucalyptus grandis milled wood lignin (MWL) was investigated by 2D 1H-13C HSQC, HMQC, and 1H-1H TOCSY correlation NMR techniques and by quantitative 13C NMR as well as by the permanganate oxidation degradation technique. The combination of 2D NMR and quantitative 13C NMR spectroscopy of nonacetylated and acetylated lignin preparations allowed reliable identification and calculation of the amount of different lignin structures. About 85% of side-chain moieties were estimated on the structural level. This information was substantiated by data on the quantity of various functional groups and interunit linkages as a whole. A modified method for calculation of the h:g:s ratio has been suggested and compared with previously suggested approaches. E. grandis MWL has been determined to have an h:g:s ratio of 2:36:62. The amounts of various phenolic/etherified noncondensed/condensed guaiacyl and syringyl moieties were approximately estimated. E. grandis MWL contained approximately 0.60/Ar of beta-O-4 moieties along with small amounts of other structural units such as pino/syringyresinol (0.03/Ar), phenylcoumaran (0.03/Ar), and spirodienone (0.05/Ar). The degree of condensation was estimated at approximately 21%; the main condensed structures are 4-O-5 moieties (approximately 0.09/Ar). The structure of E. grandis MWL was compared with those of other lignin preparations isolated from various hardwoods.
Knowledge discovery in time series databases (2001)

Adding the dimension of time to databases produces time series databases (TSDB) and introduces new aspects and difficulties to data mining and knowledge discovery. In this correspondence, we introduce a general methodology for knowledge discovery in TSDB. The process of knowledge discovery in TSDR includes cleaning and filtering of time series data, identifying the most important predicting attributes, and extracting a set of association rules that can be used to predict the time series behavior in the future. Our method is based on signal processing techniques and the information-theoretic fuzzy approach to knowledge discovery. The computational theory of perception (CTP) is used to reduce the set of extracted rules by fuzzification and aggregation. We demonstrate our approach on two types of time series: stock-market data and weather data.
Scalable and progressive audio codec (2002)

A source coding technique for variable, bandwidth-constrained channels such as the Internet must do two things: offer high quality at low data rates, and adapt gracefully to changes in available bandwidth. Here we propose an audio coding algorithm that is superior on both counts. It is inherently scalable, meaning that channel conditions can be matched without the need for additional computation. Moreover, it is compact: in subjective tests our algorithm, coded at 32 kb/s/channel, outperformed MPEG-1 Layer 3 (MP3) coded at 56 kb/s/channel (both at 44.1 kHz). We achieve this simultaneous increase in compression and scalability through use of a two-dimensional transform that concentrates relevant information into a small number of coefficients.
Purification, separation and characterization of phosphoglucomutase and phosphomannomutase from maize leaves (1998)

Different phosphomutases-phosphoglucomutase (EC 2.7.5.1; PGM) and phosphomannomutase (EC 2.7.5.7; PMM) from maize (Zea mays L.) leaves have been purified. PGM and PMM were completely separated from each other. The purified PGM was shown to be electrophoretically homogeneous. The PGM from maize leaves was found to be a homodimer with an apparent molecular mass of 132 kDa, the size of the subunits was 66 kDa. The PGM is a bifunctional enzyme, which can use both glucose-1-phosphate and mannose-1-phosphate as substrates. In contrast, the PMM appears to be monospecific for mannose-1-phosphate. Evidence is presented that PMM differs from PGM. Some properties of the maize leaves PGM and PMM differ in many respects (K(m) for substrates, pH optimum). However, some properties of PGM and PMM were similar (influence of Mg2+ and Mn2+ ions).
Transient protein expression in three Pisum sativum (green pea) varieties (2009)

The expression of proteins in plants both transiently and via permanently transformed lines has been demonstrated by a number of groups. Transient plant expression systems, due to high expression levels and speed of production, show greater promise for the manufacturing of biopharmaceuticals when compared to permanent transformants. Expression vectors based on a tobacco mosaic virus (TMV) are the most commonly utilized and the primary plant used, Nicotiana benthamiana, has demonstrated the ability to express a wide range of proteins at levels amenable to purification. N. benthamiana has two limitations for its use; one is its relatively slow growth, and the other is its low biomass. To address these limitations we screened a number of legumes for transient protein expression. Using the alfalfa mosaic virus (AMV) and the cucumber mosaic virus (CMV) vectors, delivered via Agrobacterium, we were able to identify three Pisum sativum varieties that demonstrated protein expression transiently. Expression levels of 420 +/- 26.24 mg GFP/kgFW in the green pea variety speckled pea were achieved. We were also able to express three therapeutic proteins indicating promise for this system in the production of biopharmaceuticals.
Role of hepatitis C virus in dual and triple hepatitis virus infection (1995)

Hepatitis B virus (HBV), hepatitis C virus (HCV), and hepatitis delta virus (HDV) share same transmission routes, thus dual or triple infection may occur and even persist in the same patients. A significant amount of literature has accumulated since the advent of HCV assays. It is pertinent to review and evaluate the clinical and virological significance of HCV in multiple hepatotropic viral infection. The reported series on seroprevalence of HCV indicate that HCV is found in more than 10% of HBV- or HDV-infected patients worldwide. Of the patients with dual or triple infection involving HCV, those having coreplication of viruses tend to have severe and progressive liver disease that is resistant to interferon therapy, in contrast with patients having a single virus infection. Paradoxically, dual or triple hepatitis virus infections are associated with viral interference. In particular, HCV exerts a suppressive effect on HBV and HDV and may enhance seroclearance of HBV antigens or even usurp the role of preexisting virus as the agent for continuing hepatitis. Although HBV and HDV may also suppress HCV, it appears to be less effective. These findings clearly suggest the necessity of monitoring patients with HBV or HDV infections. In view of complex dynamism of viral interaction in multiple hepatotropic virus infection, the importance of HCV assay in the clinical studies can not be overemphasized. The basic mechanisms that regulate the viral interactions, in particular the impact of HCV in dual or triple virus infections, remain to be investigated. (Hepatology 1995; 22:1101-1108.).
The role of epigenetics in mental disorders. (2007)

It is well established that the idiopathic mental disorders have a genetic basis. Yet, genetic mapping has not definitively identified any genetic mutation or polymorphism underlying these disorders. This review discusses the role of epigenetics in the pathogenesis of the idiopathic mental disorders. Epimutations and epigenetic polymorphisms are emphasized as being an interface between the genes underlying the idiopathic mental disorders and the environment. Psychosocial factors are described as important environmental factors involved in the pathogenesis of the idiopathic mental disorders, modifying the underlying genes by epigenetic mechanisms. Epigenetic strategies to identify the genes underlying the idiopathic mental disorders are described and the available molecular evidence supporting an epigenetic pathogenesis for these disorders is discussed. It also discusses the role of epigenetic factors in the pathogenesis of neuropsychiatric disorders and the relevance of the new therapeutic option, epigenetic therapy, in treating the idiopathic mental disorders and the neuropsychiatric disorders.
Bayesian classification of protein structural elements (2002)

Heuristic Bayesian classification is applied to the problem of defining and enumerating structural motifs present in protein macromolecular systems. The classification system used, Autoclass III, estimates the most probable number of classes and describes class membership in terms of both mean attribute value and expected variance. A database of 9556 protein structure segments, each five amino acids in length, was compiled from high resolution, high quality X-ray crystal structures in the Brookhaven Protein Databank. Coordinates for these segments were transformed to a translationally and rotationally invariant local reference frame, then classified.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Extraordinary Photoresponse in Two-Dimensional In2Se3 Nanosheets (2013)

We demonstrate extraordinary photoconductive behavior in two-dimensional (2D) crystalline indium selenide (In2Se3) nanosheets. Photocurrent measurements reveal that semiconducting In2Se3 nanosheets have an extremely high response to visible light, exhibiting a photoresponsivity of 3.95 × 10(2) A·W(-1) at 300 nm with an external quantum efficiency greater than 1.63 × 10(5) % at 5 V bias. The key figures-of-merit exceed that of graphene and other 2D material-based photodetectors reported to date. In addition, the photodetector has a fast response time of 1.8 × 10(-2) s and a specific detectivity of 2.26 × 10(12) Jones. The photoconductive response of α-In2Se3 nanosheets extends into ultraviolet, visible, and near-infrared spectral regions. The high photocurrent response is attributed to the direct band gap (EG = 1.3 eV) of In2Se3 combined with a large surface-area-to-volume ratio and a self-terminated/native-oxide-free surface, which help to reduce carrier recombination while keeping fast response, allowing for real-time detection under very low-light conditions.
ON THE EXISTENCE OF ENERGETIC ATOMS IN THE UPPER ATMOSPHERE OF EXOPLANET HD209458b (2010)

International audience
Cold fusion: the scientific fiasco of the century (1992)

Press conference prior events confirmations, retractions and confusion a panel is appointed hearing before a Government committee cold fusion frenzy peaks publication of the panel's report where are the fusion products? promotion of claims for cold fusion Utah born and bred cold fusion and polywater pathological science lessons appendices: University of Utah press release, Energy Research Advisory Board cold fusion panel, chronology of the cold fusion saga.
Characterization of a bovine synovial fluid lubricating factor III. The interaction with hyaluronic acid (1992)

Although hyaluronate is not a boundary lubricant in cartilaginous and latex:glass bearings, a distinct interaction with purified synovial lubricating factor (PSLF) was demonstrated by three means: 1) enhancement of lubricating ability in an artificial test system; 2) viscometry; 3) electron microscopy. The interaction was of a physical rather than a specific chemical type; it varied with the degree of purification of PSLF and of hyaluronate. The interaction accounts for retention of the relatively small PSLF molecule (approximately 280 kDa) with the synovial mucin on a 0.22 microns filter. The data provide evidence that hyaluronate and PSLF act synergistically in the boundary lubricating activity of animal joints.
A Novel Probiotic Mixture Exerts a Therapeutic Effect on Experimental Autoimmune Encephalomyelitis Mediated by IL-10 Producing Regulatory T Cells (2010)

Our data provide evidence showing that the therapeutic effect of the chosen mixture of probiotic lactobacilli was associated with induction of transferable tolerogenic Tregs in MLNs, but also in the periphery and the CNS, mediated through an IL-10-dependent mechanism. Our findings indicate a therapeutic potential of oral administration of a combination of probiotics and provide a more complete understanding of the host-commensal interactions that contribute to beneficial effects in autoimmune diseases.
Long-term repopulating abilities of enriched fetal liver stem cells measured by competitive repopulation. (1995)

To characterize hematopoietic cell biology, many investigators have used protocols that enrich for primitive hematopoietic stem cells (PHSC). In this study, we quantified the long-term repopulating ability (LTRA) of enriched and discarded fractions of PHSC from day-14 murine fetal liver using the competitive repopulation assay. We fractionated populations of fetal cells using the antigenic markers AA4.1+, AA4.1+/Sca+, and AA4.1+/Linlow/Sca+. Differentiating and repopulating abilities of each of these populations were directly compared using competitive repopulation. Adult bone marrow was mixed with fetal cell fractions from congenic donors having genetically distinguishable markers, and mixtures were given to irradiated recipients. Differentiating and repopulating abilities of the enriched donor cells were measured by the proportions of myeloid and lymphoid cells having donor markers that repopulated the recipients. LTRA was found primarily in the AA4.1+ and AA4.1+/Sca+ subpopulations. Further fractionation of the AA4.1+ cells to derive an AA4.1+/Linlow/Sca+ fraction showed that virtually all of the long-term stem cell activity was found in this subpopulation. These cells were 1400- to 1600-fold enriched in long-term functional ability compared to fresh marrow. This very high multilineage repopulating ability per cell was directly measured using a long-term functional assay in vivo. Importantly, the measured repopulating ability for AA4.1+/Linlow/Sca+ cells was about five-fold less than expected from the fraction of cells enriched and remained two- to three-fold less even after compensating for repopulating ability in discarded fractions. This illustrates that long-term functional abilities of enriched PHSC cannot be estimated from fractions enriched but should be quantitatively assayed.
Dual role of tumor necrosis factor-alpha in angiogenesis. (1992)

The role of tumor necrosis factor-alpha (TNF; cachectin) in angiogenesis has been controversial. In vitro TNF inhibits proliferation of endothelial cells (EC) whereas in the cornea it appears to stimulate vessel growth. The authors tested TNF in their recently developed disc angiogenesis system (DAS), designed to measure the proliferation of microvessels. The DAS, implanted subcutaneously in mice, was either fitted with a central pellet containing mouse recombinant TNF (mrTNF), or exposed to mrTNF delivered subcutaneously by an osmotic minipump. Low doses of mrTNF (0.01-1 ng) induced angiogenesis, which was maximum at 0.1 ng, whereas high doses (1, and 5 micrograms) inhibited it. Subcutaneous mrTNF delivered at the (high) rate of 15-60 ng/hr for 14 days inhibited angiogenesis. These results indicate bimodal, dose-dependent opposing effects and explain some of the in vitro versus in vivo paradoxical results. TNF (native or exogenous) may have opposing effects on microvessels of neoplasms and inflammatory reactions, depending on its local tissue concentrations.
Production of immunologically active surface antigens of hepatitis B virus by Escherichia coli. (1981)

Several plasmids have been constructed which direct the synthesis of hepatitis B virus surface antigens in Escherichia coli either as the native polypeptide or fused to other plasmid encoded polypeptides. When injected into rabbits, extracts from bacteria carrying some of these plasmids induced the synthesis of antibodies to the antigens even though the extracts did not give satisfactory positive results in radioimmunoassay for them. Either the NH2-terminal segment or the COOH-terminal segment of the surface antigens alone was sufficient to elicit the immune response, but antibodies against the two segments showed different specificities. The results emphasize the value of an in vivo assay for the presence of antigens in crude cell extracts and illustrate the feasibility of this type of screening with laboratory animals.
Synthesis and Structure of Fused α-Oligothiophenes with up to Seven Rings (2005)

To combine the stability of alpha-oligothiophenes with the planarity of acenes, fully fused oligothienoacenes were synthesized and their properties compared to the nonfused alpha-oligothiophenes. By employing removable solubilizing groups, our synthetic methodology made it possible to efficiently prepare and purify oligothienoacenes with up to seven fused rings. The key steps involved the halogen dance reaction and Pd-catalyzed coupling of Bu3SnSSnBu3 to introduce sulfur linkages. This approach eliminates alpha-beta anion equilibration, a significant improvement over the traditional method of introducing sulfur linkages via Li-Br exchange. X-ray diffraction data indicate that pentathienoacene and heptathienoacene adopt pi-stacked packing motifs in contrast to the herringbone packing of nonfused oligothiophenes. On the basis of the linear dependence of the longest lambdamax on the reciprocal number of double bonds of thienoacenes with three to seven rings, the band gap of polythienoacene is extrapolated to be 2.21 eV.
Bcl-xL is an oncogenic driver in colorectal cancer (2016)

Colorectal cancer (CRC) is the second most common malignant neoplasia in women and men worldwide. The B-cell lymphoma 2 (Bcl-2) protein family is mainly known for its pivotal role in the regulation of the mitochondrial death pathway. Anti-apoptotic Bcl-2 proteins may provide survival benefits and induce therapy resistance in cancer cells. Among anti-apoptotic Bcl-2 proteins, we found solely Bcl-xL strongly upregulated in human CRC specimens. In order to study protein function in the context of tumor initiation and progression in vivo, we generated a mouse model lacking Bcl-xL in intestinal epithelial cells (Bcl-xL(IEC-KO)). If challenged in an inflammation-driven tumor model, Bcl-xL(IEC-KO) mice showed a significantly reduced tumor burden with lower tumor numbers per animal and decreased tumor sizes. Analysis of cell death events by immunohistochemistry and immunoblotting revealed a striking increase of apoptosis in Bcl-xL-negative tumors. qRT-PCR and immunohistochemistry excluded changes in proliferative capacity and immune cell infiltration as reasons for the reduced tumor load and thereby identify apoptosis as key mechanism. Human CRC tissue was cultured ex vivo and treated with the small molecule compound ABT-737, which inhibits Bcl-xL and Bcl-2. Under ABT-737 treatment, the amount of apoptotic tumor cells significantly increased compared with controls, whereas proliferation levels remained unaltered. In summary, our findings identify Bcl-xL as a driver in colorectal tumorigenesis and cancer progression, making it a valuable target for clinical application.
Specific interactions of liposomes with PMN leukocytes upon incorporating tuftsin in their bilayers (1984)

Incorporation of tuftsin derivatives, Thr-Lys-Pro-Arg-NH-C18H37 or Thr-Lys-Pro-Arg-NH-(CH2)2-NH-COC15H31, into an egg phosphatidylcholine/cholesterol liposome bilayer led to significantly enhanced binding of the liposomes to PMN leukocytes at 37 degrees C but not at 0 degree C. Under identical conditions, no such enhanced binding of the liposomes was observed with erythrocytes and lymphocytes. These results demonstrate that grafting of tuftsin on the liposome bilayer enables the liposome to recognize specifically the PMN leukocytes and to deliver its contents to these cells.
Effect of environment on extremely severe road traffic crashes: retrospective epidemic analysis during 2000-2001. (2004)

Extremely severe RTCs are closely related with environmental factors. Rational road programming, enhancing road establishment and improving road conditions are probably effective measures to reduce the road traffic injuries.