gebhart/patrepeval huggingface dataset:
Systemic Analysis of PPARγ in Mouse Macrophage Populations Reveals Marked Diversity in Expression with Critical Roles in Resolution of Inflammation and Airway Immunity (2012)

Although peroxisome proliferator-activated receptor γ (PPARγ) has anti-inflammatory actions in macrophages, which macrophage populations express PPARγ in vivo and how it regulates tissue homeostasis in the steady state and during inflammation remains unclear. We now show that lung and spleen macrophages selectively expressed PPARγ among resting tissue macrophages. In addition, Ly-6C(hi) monocytes recruited to an inflammatory site induced PPARγ as they differentiated to macrophages. When PPARγ was absent in Ly-6C(hi)-derived inflammatory macrophages, initiation of the inflammatory response was unaffected, but full resolution of inflammation failed, leading to chronic leukocyte recruitment. Conversely, PPARγ activation favored resolution of inflammation in a macrophage PPARγ-dependent manner. In the steady state, PPARγ deficiency in red pulp macrophages did not induce overt inflammation in the spleen. By contrast, PPARγ deletion in lung macrophages induced mild pulmonary inflammation at the steady state and surprisingly precipitated mortality upon infection with Streptococcus pneumoniae. This accelerated mortality was associated with impaired bacterial clearance and inability to sustain macrophages locally. Overall, we uncovered critical roles for macrophage PPARγ in promoting resolution of inflammation and maintaining functionality in lung macrophages where it plays a pivotal role in supporting pulmonary host defense. In addition, this work identifies specific macrophage populations as potential targets for the anti-inflammatory actions of PPARγ agonists.
마이봄선 기능이상의 평가와 이에 따른 눈물막 파괴검사 및 Schirmer Test 의 결과비교 (2000)

1999년 3월부터 8월까지 본원 안과에 내원한 환자 75명 150안을 대상으로 마이봄선 촬영술 및 압박배출술을 시행하여 마이봄선 개구부폐쇄가 있는 1군, 마이봄선 파괴가 있는 2군,위의 두 가지가 복합되어 있는 3군, 정상인 4군으로 분류하였으며, 각 군에서 눈물막 파괴검사, Schirmer test, 환자의 증상을 서로 비교하여 상관관계를 알아보았다. 눈물막 파괴검사는 1,2,3,4군에서 각각 평균 8.20±2.6, 7.70±2.12, 6.22±2.07, 14.56±2.55초이었고,환자가 호소하는 증상의 개수는 2.10 ±1.06, 2.33 ±0.97, 2.92 ±0.77, 0.72 ±0.71개로 나타났으며, 서로간에 통계학적으로 유의한 차이를 보였다(p＜0.05). Schirmer test에서는 각각 14.75±3.86, 15.57±4.43, 18.13±4.05, 17.27±4.21 mm로 나타났지만 통계적 유의성은 없었다(p＞0.05). 이상의 결과로 보아 마이봄선 기능이상이 눈물막 파괴검사의 결과치를 낮게 하고, 환자로 하여금 건성안의 증상을 느끼게 하는 것을 확인할 수 있었고, 저자들은 외래에서 간단하게 이용할 수 있는 마이봄선 촬영술과 마이봄선 압박배출이 마이봄선 기능이상의 정도를 비교적 객관적으로 평가할 수 있는 좋은 검사라고 생각되며, 건성안 환자에게 이 검사를 시행하여 마이봄선 기능이상을 평가하는 것이 원인 및 치료에 도움을 줄 것으로 생각된다(한안지 41:1875~1882,2000).
Application of the Principle of Hard and Soft Acids and Bases to Organic Chemistry (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTApplication of the Principle of Hard and Soft Acids and Bases to Organic ChemistryRalph G. Pearson and Jon. SongstadCite this: J. Am. Chem. Soc. 1967, 89, 8, 1827–1836Publication Date (Print):April 1, 1967Publication History Published online1 May 2002Published inissue 1 April 1967https://pubs.acs.org/doi/10.1021/ja00984a014https://doi.org/10.1021/ja00984a014research-articleACS PublicationsRequest reuse permissionsArticle Views4473Altmetric-Citations790LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The inflammatory biomarker YKL‐40 at admission is a strong predictor of overall mortality (2012)

The level of YKL-40 at admission is a strong predictor of overall mortality, independent of diagnosis and could be useful as a biomarker in the acute evaluation of all patients.
Somatic antigens of Pseudomonas aeruginosa (1987)

O-specific polysaccharides, obtained on mild acid degradation of lipopolysacchrides of the serologically related strains Pseudomonas aeruginosa O3 (Lányi classification), O25 (Wokatsch classification) and immunotypes 3 and 7 (Fisher classification), are built up of trisaccharide repeating units involving 2-acetamido-2,6-dideoxy-D-galactose (N-acetyl-D-fucosamine), 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid or 2,3-diacetamido-2,3-dideoxy-L-guluronic acid and 3-acetamidino-2-acetamido-2,3-dideoxy-D-mannuronic acid or 3-acetamidino-2-acetamido-2,3-dideoxy-L-guluronic acid. Lányi O3(a),3d,3f and Wokatsch O25 polysaccharides contain also O-acetyl groups. On the basis of solvolysis with anhydrous hydrogen fluoride, resulting in trisaccharide fragments with N-acetylfucosamine residue at the reducing terminus, chemical modifications of the acetamidino group (alkaline hydrolysis to the acetamido group or reductive deamination to the ethylamino group), as well as analysis by 1H-NMR (including nuclear Overhauser effect experiments) and 13C-NMR spectroscopy, and fast-atom bombardment mass spectrometry, it was concluded that the repeating units of the polysaccharides have the following structures: (Formula: see text) where HexNAcAmA = alpha-L-GulNAcAmA (approximately 70%) or beta-D-ManNacAMA (approximately 30%). Lányi O3(a),3d,3f polysaccharide involves two types of repeating units, which differ from each other only in the configuration at C-5 of the 3-acetamidino-2-acetamido-2,3-dideoxyuronic acid residue. Lányi O3(a),3c,O3a,3d,3e and Fisher immunotypes 3 and 7 polysaccharides contain, together with the major repeating units shown above, a small proportion of units in which the derivative of alpha-L-guluronic acid is replaced by the corresponding beta-D-manno isomer. The data obtained provide the opportunity to substantiate the serological interrelations between these strains of P. aeruginosa by the presence in the O-specific polysaccharides of common monosaccharides or disaccharide fragments. The distinctions between them stem from the presence or absence of the O-acetyl group, a different configuration of the glycosidic linkage of the N-acetylfucosamine residue and/or a different configuration at C-5 of one or both derivatives of diaminouronic acids.
Adhesion and erythrophagocytosis of human senescent erythrocytes by autologous monocytes and their inhibition by beta-galactosyl derivatives. (1986)

Senescent human erythrocytes (RBC) are able to adhere to and be phagocytized by autologous monocytes in vitro to a greater extent than are young RBC. This adhesion and erythrophagocytosis of senescent RBC is inhibited by D-galactose, N-acetyl-D-galactosamine, their corresponding derivatives of bovine serum albumin, and lactose. On the other hand, D-glucose, D-mannose, L-fucose, N-acetyl-D-glucosamine, and their corresponding derivatives of bovine serum albumin are noninhibiting. The glycopeptides released by tryptic digestion of senescent RBC and purified on immobilized peanut agglutinin are the most effective inhibitors of both RBC adhesion and phagocytosis by autologous monocytes obtained from peripheral blood.
Aberrant Cell Expansion in the elongation Mutants of Barley (2009)

The elongation (elo) mutants of barley (Hordeum vulgare cv 'Himalaya') are a class of dwarf plants with defects affecting cell expansion. The phenotypes of mutants in three of the elo loci (elo1, elo2 and elo3) are recessive to the wild-type allele, and the mutations at elo-4 and elo-5 are semi-dominant. Allelism tests showed that elo1, elo2 and elo3 were at separate loci, and mapping data indicated that elo-5 was possibly allelic to either elo1 or elo2. A phenotype common to all elo mutants was the presence of short, radially swollen cells on the leaf epidermis, indicating a defect in longitudinal cell expansion. In three of the mutants, elo1, elo3 and elo5, this was accompanied by a twisting growth habit. Two of the mutations, elo2 and elo-5, affected cell division, with aberrant periclinal cell division resulting in the formation of increased cell layers in the leaf epidermis of elo2 and elo-5 homozygotes and in the aleurone layer of elo2 grains. Misplaced anticlinal divisions also occurred in the elo-5 leaf epidermis. Leaf cell walls of all elo lines contained less cellulose than the wild- type, and the cortical microtubules in elongating root epidermal cells in some elo lines were more randomly oriented than in the wild-type, consistent with the presence of radially swollen cells. We discuss possible functions for the Elo genes in primary cell wall synthesis.
Heterocyclische Spiroverbindungen: Spiro [benzo[b] thiophen‐4(5H),3′ ‐pyrrolidine] (1984)

Abstract Im Rahmen unserer längerfristigen Projekte zur Synthese heterocyclischer Spiroverbindungen wurden die Substanzen der allgemeinen Formeln I und II entsprechend Formelschemata 1 und 2 synthetisiert. Das spektroskopische Verhalten ( 1 H‐, 13 C‐NMR) dieser beiden Verbindungstypen wurde untersucht.
Overexpression of stromelysin-3, BM-40/SPARC, and MET genes in human esophageal carcinoma: implications for prognosis. (1998)

Molecular markers can improve staging and predict aggressive clinical behavior in esophageal cancer, thus helping to define appropriate therapeutic protocols and to identify patients who will benefit from surgery. We therefore characterized, by Northern blot and/or immunohistochemistry, the relative expression of three effectors involved in the invasion, angiogenesis, and dissemination of tumor cells in esophageal cancer versus nontumoral mucosae: (a) stromelysin-3 (ST3), a member of the metalloproteinase family; (b) basement membrane 40/secreted protein acidic and rich in cysteine (BM-40/SPARC), an extracellular matrix-associated protein involved in angiogenesis; and (c) the hepatocyte growth factor receptor MET, which triggers the scattering of epithelial cells. Results were analyzed in relation to clinicopathological parameters (cpTNE) including tumor size (T), lymph node status (N), periesophageal tissue invasion (E), disease recurrence, and overall survival. The ST3, BM-40/SPARC, and MET genes were found to be overexpressed in tumor samples compared to control mucosa. BM-40/SPARC and MET mRNA levels were not linked to any one of the cpTNE, indicating that this overexpression occurs at an early stage of neoplastic progression. In contrast, ST3 expression, identified by immunohistochemistry in fibroblastic cells surrounding neoplastic islets, correlated with tumor size and periesophageal tissue invasion. Of the 36 patients studied, those with high ST3 levels had shorter disease-free survival than those with low levels, but there was no relationship between the cpTNE and disease recurrence or survival. Our study demonstrates that ST3, BM-40/SPARC, and MET are involved in different steps of esophageal carcinogenesis and that ST3 overexpression is a marker of aggressive clinical behavior. We conclude that in esophageal cancer, ST3 might help to assess survival and the risk of recurrence after surgical resection.
Effects of wavelength routing and selection algorithms on wavelength conversion gain in WDM optical networks (1998)

Wavelength-division multiplexing (WDM) technology is emerging as the transmission and switching mechanism for future optical mesh networks. In these networks it is desired that a wavelength can be routed without electrical conversions. Two technologies are possible for this purpose: wavelength selective cross-connects (WSXC) and wavelength interchanging cross-connects (WIXC), which involve wavelength conversion. It is believed that wavelength converters may improve the blocking performance, but there is a mix of results in the literature on the amount of this performance enhancement. We use two metrics to quantify the wavelength conversion gain: the reduction in blocking probability and the increase in maximum utilization, compared to a network without converters. We study the effects of wavelength routing and selection algorithms on these measures for mesh networks. We use the overflow model to analyze the blocking probability for wavelength-selective (WS) mesh networks using the first-fit wavelength assignment algorithm. We propose a dynamic routing and wavelength selection algorithm, the least-loaded routing (LLR) algorithm, which jointly selects the least-loaded route-wavelength pair. In networks both with and without wavelength converters the LLR algorithm achieves much better blocking performance compared to the fixed shortest path routing algorithm. The LLR produces larger wavelength conversion gains; however, these large gains are not realized in sufficiently wide utilization regions and are diminished with the increased number of fibers.
Role of interleukin‐1 and tumor necrosis factor α in matrix degradation of human osteoarthritic cartilage (2005)

These results suggest that the autocrine/paracrine activities of TNFalpha and IL-1 in articular cartilage may play important roles in cartilage matrix degradation in OA patients but not in all patients. Inhibition of either or both of these cytokines may offer a useful therapeutic approach to the management of OA by reducing gene expression of MMPs involved in cartilage matrix degradation and favoring its repair.
The Mitogen-activated Protein Kinase Phosphatase-3 N-terminal Noncatalytic Region Is Responsible for Tight Substrate Binding and Enzymatic Specificity (1998)

We have reported recently that the dual specificity mitogen-activated protein kinase phosphatase-3 (MKP-3) elicits highly selective inactivation of the extracellular signal-regulated kinase (ERK) class of mitogen-activated protein (MAP) kinases (Muda, M., Theodosiou, A., Rodrigues, N., Boschert, U., Camps, M., Gillieron, C., Davies, K., Ashworth, A., and Arkinstall, S. (1996) J. Biol. Chem. 271, 27205-27208). We now show that MKP-3 enzymatic specificity is paralleled by tight binding to both ERK1 and ERK2 while, in contrast, little or no interaction with either c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK) or p38 MAP kinases was detected. Further study revealed that the N-terminal noncatalytic domain of MKP-3 (MKP-3DeltaC) binds both ERK1 and ERK2, while the C-terminal MKP-3 catalytic core (MKP-3DeltaN) fails to precipitate either of these MAP kinases. A chimera consisting of the N-terminal half of MKP-3 with the C-terminal catalytic core of M3-6 also bound tightly to ERK1 but not to JNK3/SAPKbeta. Consistent with a role for N-terminal binding in determining MKP-3 specificity, at least 10-fold higher concentrations of purified MKP-3DeltaN than full-length MKP-3 is required to inhibit ERK2 activity. In contrast, both MKP-3DeltaN and full-length MKP-3 inactivate JNK/SAPK and p38 MAP kinases at similarly high concentrations. Also, a chimera of the M3-6 N terminus with the MKP-3 catalytic core which fails to bind ERK elicits non selective inactivation of ERK1 and JNK3/SAPKbeta. Together, these observations suggest that the physiological specificity of MKP-3 for inactivation of ERK family MAP kinases reflects tight substrate binding by its N-terminal domain.
Dynamic Updating of Reservoir Models (2009)

Abstract In sandstone reservoirs, one of the most important challenges is the effective upscaling of a fine scale model. A conventional upscaling process is not adequate when significant percentage of shale distribution exists in the reservoir. In the fine scale model, significant discontinuity exists in sand bodies. Some sand bodies are connected to the wells, and some are not. As the fine scale model is upscaled, some discontinuous sand bodies are combined with other connected sands. This results in two potential problems: the connected volume to the existing wells increases, thus making the production from those wells more optimistic, and the production from in fill wells do not show as much additional potential since some of the new volumes which should have been connected to the new well are already drained by the existing wells. A new procedure1 is developed to overcome this problem. In a new procedure, we first determine the connected sand volume to the existing wells. We remove the dis-connected sand bodies from the fine scale model. We then upscale the model to a desired level. We simulate the flow performance till a desired time when either new wells are drilled or some well are shut-in. At that point, we start from the fine scale model again and determine new connected volume due to additional of new wells. We combine the new virgin sands with depleted sands in the upscaled model and determine the saturation and pressure in the upscaled model using an appropriate material balance technique. We re-start the simulation using newly connected volume till we reach a point of drilling additional wells. The key difference between the proposed method and the existing methods, is our ability to add new hydrocarbon volumes (as well as new conductivity) in the model as a function of time. The proposed method was applied to a giant oil field in Siberia which is in turbite environment with large amounts of discontinuous sand bodies. We were able to demonstrate the advantage of the proposed method by comparing the performance of upscaled simulation model to the fine scale geologic model. As the percentage of sand decreases in a given reservoir, the difference between the conventional and a proposed method becomes significant. Using the new approach, we would be able to evaluate the infill potential much more accurately.
Minimally Invasive Percutaneous Posterior Lumbar Interbody Fusion (2002)

Abstract OBJECTIVE The wide exposure required for a standard posterior lumbar interbody fusion (PLIF) can cause unnecessary trauma to the lumbar musculoligamentous complex. By combining existing microendoscopic, percutaneous instrumentation and interbody technologies, a novel, minimally invasive, percutaneous PLIF technique was developed to minimize such iatrogenic tissue injury (MIP-PLIF). METHODS The MIP-PLIF technique was validated in three cadaveric torsos with six motion segments decompressed and fused. Preoperative variables measured from imaging included interpedicular distance, pedicular height and width, interspinous distance, lordosis, intervertebral height, Cobb angle, and foraminal height and volume. Using the METRx and MD spinal access systems (Medtronic Sofamor Danek, Memphis, TN), bilateral laminotomies were performed using a hybrid of microsurgical and microendoscopic techniques. The intervertebral disc spaces were then distracted and prepared with the Tangent (Medtronic Sofamor Danek) interbody instruments. Either a 10 or 12 by 22 mm interbody graft was then placed. Using the Sextant (Medtronic Sofamor Danek) system, percutaneous pedicle screw-rod fixation of the motion segment was completed. We then applied MIP-PLIF in three patients. RESULTS For segments with preoperative intervertebral/foraminal height loss, MIP-PLIF was effective in restoring both heights in all cases. The amount of improvement (9.7 to 38% disc height increase; 7.7 to 29.9% foraminal height increase) varied directly with the size of the graft used and the original degree of disc and foraminal height loss. Segmental lordosis improved by 29% on average. Graft and screw placement was accurate in the cadavers, except for a single Grade 1 screw violation of one pedicle. The average operative time was 3.5 hours per level. In our three clinical cases, the MIP-PLIF procedure required a mean of 5.4 hours, estimated blood loss was 185 ml, and inpatient stay was 2.8 days, with no intravenous narcotic use after 2 days in any of the patients. All screw and graft placements were confirmed. CONCLUSION A complete PLIF procedure can be safely and effectively performed using minimally invasive techniques, thereby potentially reducing the pain and morbidity associated with standard open surgery. Prospective, randomized outcome studies will be required to validate the efficacy of this exciting new surgical technique.
Glutathione-S-transferase M1, M3, P1 and T1 polymorphisms and severity of lung disease in children with cystic fibrosis (2004)

These data provide the first evidence suggesting that polymorphism of the GSTM3 gene contributes to clinical severity in CF, which may have prognostic significance and could prompt to start a more targeted therapy in young patients with CF.
Natural Metabolic Diversity Encoded by the Enterocin Biosynthesis Gene Cluster (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTNatural Metabolic Diversity Encoded by the Enterocin Biosynthesis Gene ClusterJörn Piel, Khanh Hoang, and Bradley S. MooreView Author Information Department of Chemistry, Box 351700 University of Washington Seattle, Washington 98195-1700 Division of Medicinal Chemistry College of Pharmacy, University of Arizona Tucson, Arizona 85721-0207 Cite this: J. Am. Chem. Soc. 2000, 122, 22, 5415–5416Publication Date (Web):May 20, 2000Publication History Received7 February 2000Published online20 May 2000Published inissue 1 June 2000https://pubs.acs.org/doi/10.1021/ja000445nhttps://doi.org/10.1021/ja000445nrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1151Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Cluster chemistry,Genetics,Liquid chromatography-mass spectrometry,Metabolism,Rearrangement Get e-Alerts
Endothelial-Mural Cell Signaling in Vascular Development and Angiogenesis (2009)

Mural cells are essential components of blood vessels and are necessary for normal development, homeostasis, and organ function. Alterations in mural cell density or the stable attachment of mural cells to the endothelium is associated with several human diseases such as diabetic retinopathy, venous malformation, and hereditary stroke. In addition mural cells are implicated in regulating tumor growth and have thus been suggested as potential antiangiogenic targets in tumor therapy. In recent years our knowledge of mural cell function and endothelial-mural cell signaling has increased dramatically, and we now begin to understand the mechanistic basis of the key signaling pathways involved. This is mainly thanks to sophisticated in vivo experiments using a broad repertoire of genetic technologies. In this review, we summarize the five currently best understood signaling pathways implicated in mural cell biology. We discuss PDGFB/PDGFRbeta- dependent pericyte recruitment, as well as the role of angiopoietins and Tie receptors in vascular maturation. In addition, we highlight the effects of sphingosine-1-phosphate signaling on adherens junction assembly and vascular stability, as well as the role of TGF-beta-signaling in mural cell differentiation. We further reflect recent data suggesting an important function for Notch3 signaling in mural cell maturation.