gebhart/patrepeval huggingface dataset:
The strength‐duration relationship for excitation of myelinated nerve: computed dependence on membrane parameters. (1983)

Thresholds to applied current pulses have been determined for the myelinated nerve model of Goldman & Albus (1968). Strength-duration curves have been plotted, and compared with three strength-duration equations that have been proposed in the past. The simple, linear relation between stimulus charge and stimulus duration proposed by Weiss (1901) provided the best fit to the computed data. The effects on the strength-duration relationship of changes in twelve parameters of the model were determined and expressed in terms of the strength-duration time constant and rheobasic current. The rheobase depended primarily on conductances, whereas the strength-duration time constant depended on the electrotonic time constant and also on the rate of sodium activation. The model predicts strength-duration curves of the same form, for extracellular or intracellular stimulation where the external resistance is low and uniform. Tripolar stimulation, with anodes over adjacent rather than remote nodes, is predicted to result in much shorter strength-duration time constants, but with a similar sensitivity to nodal membrane parameters. The limitations of strength-duration measurements on myelinated nerves are discussed in the light of these simulations.
Verticillin A Overcomes Apoptosis Resistance in Human Colon Carcinoma through DNA Methylation-Dependent Upregulation of BNIP3 (2011)

Drug resistance is a major cause of failure in cancer chemotherapy. Therefore, identification and combined use of adjuvant compounds that can overcome drug resistance may improve the efficacy of cancer therapy. We screened extracts of Verticillium species-infected mushrooms for antitumor compounds and identified the compound Verticillin A as an inducer of hepatoma cell apoptosis in vitro and an inhibitor of tumor xenograft growth in vivo. Verticillin A exhibited a potent apoptosis-sensitizing activity in human colon carcinoma cells exposed to TRAIL or Fas in vitro. Furthermore, Verticillin A effectively sensitized metastatic human colon carcinoma xenograft to TRAIL-mediated growth inhibition in vivo. At the molecular level, we observed that Verticillin A induces cell-cycle arrest in the G₂ phase of the cell cycle in human colon carcinoma cells, markedly upregulating BNIP3 in both hepatoma and colon carcinoma cells. Notably, silencing BNIP3 decreased the sensitivity of tumor cells to Verticillin A-induced apoptosis in the absence or presence of TRAIL. We found that the BNIP3 promoter is methylated in both human hepatoma and colon carcinoma cells and tumor specimens. Verticillin A upregulated the expression of a panel of genes known to be regulated at the level of DNA methylation, in support of the concept that Verticillin A may act by demethylating the BNIP3 promoter to upregulate BNIP3 expression. Taken together, our findings identify Verticillin A as a potent apoptosis sensitizer with great promise for further development as an adjuvant agent to overcome drug resistance in human cancer therapy.
Beyond short snippets: Deep networks for video classification (2015)

Convolutional neural networks (CNNs) have been extensively applied for image recognition problems giving state-of-the-art results on recognition, detection, segmentation and retrieval. In this work we propose and evaluate several deep neural network architectures to combine image information across a video over longer time periods than previously attempted. We propose two methods capable of handling full length videos. The first method explores various convolutional temporal feature pooling architectures, examining the various design choices which need to be made when adapting a CNN for this task. The second proposed method explicitly models the video as an ordered sequence of frames. For this purpose we employ a recurrent neural network that uses Long Short-Term Memory (LSTM) cells which are connected to the output of the underlying CNN. Our best networks exhibit significant performance improvements over previously published results on the Sports 1 million dataset (73.1% vs. 60.9%) and the UCF-101 datasets with (88.6% vs. 88.0%) and without additional optical flow information (82.6% vs. 73.0%).
LAP, a novel member of the C/EBP gene family, encodes a liver-enriched transcriptional activator protein. (1990)

A gene, encoding a liver-enriched transcriptional activator protein (LAP) has been isolated. LAP is a 32-kD protein that stimulates the transcription of chimeric genes containing albumin D-promoter elements both in vivo and in vitro. LAP shares extensive sequence homology (71%) in its DNA-binding and leucine zipper domains with C/EBP. As a consequence, these two proteins show an indistinguishable DNA-binding specificity and readily heterodimerize. In addition, both genes, lap and cebp, are devoid of intervening sequences. Although correctly initiated transcripts from the LAP gene accumulate in the six examined tissues--liver, lung, spleen, kidney, brain, and testis--LAP protein is highly enriched in liver nuclei. Thus, the preferential accumulation of LAP protein in liver appears to be regulated post-transcriptionally.
The role of neutrophils and platelets in a rabbit model of thromboembolic stroke. (1991)

Cerebral ischemia is accompanied by many of the cardinal features of acute inflammation such as neutrophil and platelet activation and accumulation. We sought to determine whether circulating neutrophils or platelets contribute to brain injury in a rabbit model of thromboembolic stroke that includes a fixed duration of superimposed systemic hypotension. We randomized 18 rabbits to receive either antineutrophil antiserum (n = 6), antiplatelet antiserum (n = 5), or nonimmune serum (n = 7). We assessed brain ischemia by measuring cerebral blood flow, intracranial pressure, and infarct size. Following the intracarotid administration of an autologous clot, cerebral blood flow in all groups fell to less than 5 ml/100 g/min during induced hypotension. After restoration of baseline blood pressure, mean cerebral blood flow in neutropenic animals recovered to 20-30 ml/100 g/min while that in control and thrombocytopenic rabbits remained at less than 10 ml/100 g/min. Intracranial pressure in control animals rose steadily to a final value of 241% of baseline, while a much smaller increase (148% of baseline) was noted in the thrombocytopenic group; no change from baseline was evident in the neutropenic group. Infarct size was significantly (p less than 0.05) reduced in the neutropenic group but not in the thrombocytopenic group. These results suggest that neutrophils may be important contributors to ischemia-induced brain injury whereas the role of platelets is more subtle.
Novel LSI/SOI wafer fabrication using device layer transfer technique (1985)

A device layer transfer technique, wherein a device layer already fabricated on a silicon wafer is transferred onto an insulating substrate, has been developed for achieving an LSI/SOI wafer. This technique is based on preferential polishing, newly developed with a more than 100 Si/SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> polishing rate ratio. A bipolar LSI active layer, formed in a 1 µm thick epitaxial layer, has been successfully transferred to a 4 inch diameter quartz glass. No change in I <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">C</inf> -V <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">CE</inf> characteristics of the transistor and leakage current of CB junction were observed. Isolation breakdown voltage was markedly improved after transfer. The device transfer technique is a promising method for realizing an LSI/SOI wafer with both large diameter and high crystal quality.
Identification and predicted sequence of a previously unrecognized small hydrophobic protein, SH, of the paramyxovirus simian virus 5 (1985)

A previously unrecognized gene (SH) has been identified on the virion RNA of the paramyxovirus simian virus 5 between the genes for the fusion protein and the hemagglutinin-neuraminidase. An SH mRNA of 292 nucleotides (plus polyadenylate residues), transcribed from the SH gene, has been identified. The SH mRNA contains a single open reading frame which encodes a polypeptide of 44 amino acids with a molecular weight of 5,012. The SH polypeptide is predicted to contain an extensive hydrophobic region. This protein has been identified in simian virus 5-infected cells, and it has been shown to be encoded by the SH mRNA by in vitro translation of size-fractionated mRNAs, hybrid-arrest translation, and hybrid-selection translation.
Effect of Ionic Strength and Serum on the Activity Profile of Bovine Testicular Hyaluronidase (1979)

Conference Article| December 01 1979 Effect of Ionic Strength and Serum on the Activity Profile of Bovine Testicular Hyaluronidase PETER GACESA; PETER GACESA 1Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, Wales, U.K. Search for other works by this author on: This Site PubMed Google Scholar MARC J. SAVITSKY; MARC J. SAVITSKY 1Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, Wales, U.K. Search for other works by this author on: This Site PubMed Google Scholar KENNETH S. DODGSON; KENNETH S. DODGSON 1Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, Wales, U.K. Search for other works by this author on: This Site PubMed Google Scholar ANTHONY H. OLAVESEN ANTHONY H. OLAVESEN 1Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, Wales, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1979) 7 (6): 1287–1289. https://doi.org/10.1042/bst0071287 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation PETER GACESA, MARC J. SAVITSKY, KENNETH S. DODGSON, ANTHONY H. OLAVESEN; Effect of Ionic Strength and Serum on the Activity Profile of Bovine Testicular Hyaluronidase. Biochem Soc Trans 1 December 1979; 7 (6): 1287–1289. doi: https://doi.org/10.1042/bst0071287 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1979 Biochemical Society1979 Article PDF first page preview Close Modal You do not currently have access to this content.
Multi-Sentence Compression: Finding Shortest Paths in Word Graphs (2010)

We consider the task of summarizing a cluster of related sentences with a short sentence which we call multi-sentence compression and present a simple approach based on shortest paths in word graphs. The advantage and the novelty of the proposed method is that it is syntax-lean and requires little more than a tokenizer and a tagger. Despite its simplicity, it is capable of generating grammatical and informative summaries as our experiments with English and Spanish data demonstrate.
A Novel Monoclonal Antibody Specific for Sialylated MUC1 Mucin (1996)

Development of a new monoclonal antibody (mAb) MY.1E12 which reacts with sialylated MUC1 mucins is described. The mAb did not react with any component in the lysates of COS-1 cells, whereas it bound to sialylated MUC1 mucins produced by COS-1 cells transiently transfected with MUC1 mucin cDNA, strongly suggesting that the expression of the epitope of mAb MY.1E12 depends on the presence of the MUC1 mucin core peptide. The requirement of sialyl residues for antibody recognition was established by Western blotting analysis of extracts of various carcinoma cells and in situ desialylation. In all cases, the mAb binding of electrophoretically separated MUC1 mucin diminished after desialylation by mild acid hydrolysis. When Capan-1 pancreatic carcinoma cells were pretreated with benzyl-N-acetylgalactosaminide in culture, the MUC1 mucins produced under these conditions, which were detected by core peptide-specific mAbs, did not react with mAb MY.1E12. These results suggest that O-linked carbohydrate chains are important for the mAb binding.
Selection‐dominant and nonaccessible epitopes on cell‐surface receptors revealed by cell‐panning with a large phage antibody library (1999)

To generate antibodies to defined cell-surface antigens, we used a large phage antibody fragment library to select on cell transfectants expressing one of three chosen receptors. First, in vitro panning procedures and phage antibody screening ELISAs were developed using whole live cells stably expressing the antigen of interest. When these methodologies were applied to Chinese hamster ovary (CHO) cells expressing one of the receptors for a neuropeptide, somatostatin, using either direct cell panning or a strategy of depletion or ligand-directed elution, many different pan-CHO-cell binders were selected, but none was receptor specific. However, when using direct panning on CHO-cells expressing the human membrane protein CD36, an extraordinary high frequency of antigen-specific phage antibodies was found. Panning on myoblasts expressing the rat homologue of CD36 revealed a similar selection dominance for anti-(CD36). Binding of all selected 20 different anti-(CD36) phage was surprisingly inhibited by one anti-(CD36) mAb CLB-IVC7, which recognizes a functional epitope that is also immunodominant in vivo. Similar inhibition was found for seven anti-(rat) CD36 that cross-reacted with human CD36. Our results show that, although cells can be used as antigen carriers to select and screen phage antibodies, the nature of the antigen target has a profound effect on the outcome of the selection.
Light Modulation of Cellular cAMP by a Small Bacterial Photoactivated Adenylyl Cyclase, bPAC, of the Soil Bacterium Beggiatoa (2010)

The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase directly linked to a BLUF (blue light receptor using FAD) type light sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated adenylyl cyclase (bPAC) showed cyclase activity that is low in darkness but increased 300-fold in the light. This enzymatic activity decays thermally within 20 s in parallel with the red-shifted BLUF photointermediate. bPAC is well expressed in pyramidal neurons and, in combination with cyclic nucleotide gated channels, causes efficient light-induced depolarization. In the Drosophila central nervous system, bPAC mediates light-dependent cAMP increase and behavioral changes in freely moving animals. bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals.
High-power-density GaAs MISFETs with a low-temperature-grown epitaxial layer as the insulator (1991)

A GaAs layer grown by molecular beam epitaxy at 200 degrees C is used as the gate insulator for GaAs MISFETs. The gate reverse breakdown and forward turn-on voltages, are improved substantially by using the high-resistivity GaAs layer between the gate metal and the conducting channel. It is shown that a reverse bias of 42 V or forward bias of 9,3 V is needed to reach a gate current of 1 mA/mm of gate width. A MISFET having a gate of 1.5*600 mu m delivers an output power of 940 mW (1.57-W/mm power density) with 4.4-dB gain and 27.3% power added efficiency at 1.1 GHz. This is the highest power density reported for GaAs-based FETs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
High-Density Lipoprotein — The Clinical Implications of Recent Studies (1989)

DESPITE early observations suggesting an inverse relation between serum levels of high-density lipoprotein (HDL) cholesterol and coronary disease,1 2 3 the possible protective role of HDL in atherogenesis received little attention until its "rediscovery" by Miller and Miller4 in 1975 and the publication of confirmatory results from the Honolulu,5 Framingham,6 and Tromsø7 heart studies in 1976 and 1977. In the ensuing decade, research into the biochemistry, metabolism, epidemiology, and genetics of HDL has expanded rapidly, but many questions remain unanswered. The recent publication of the results of the Helsinki Heart Study8 , 9 — in which simultaneous 11 percent increases in HDL and reductions . . .
Reversible Formation and Dissolution of Silver Nanoparticles in Aqueous Surfactant Media (1997)

Nanosize silver particles are formed in aqueous surfactant systems and have been found to be very reactive toward oxygen in the presence of borohydride. Purging of air (O2) or simple shaking leads to complete dissolution of silver particles, which is further regenerated on standing. Thus an oscillation between a yellow silver plasmon band and colorless solution is observed on periodic shaking. This oscillation continues as long as excess borohydride is present. Investigation shows that borohydride, which is a strong nucleophile, adsorbs on the surface of the silver particle and decreases the reduction potential of the silver particle so that it reacts rapidly with oxygen. Surfactant plays an extra role over particle stabilization. It removes the oxidized silver from the particle surface to the bulk and also retards the rate of reduction of AgI to Ag0.
Cytokines and Estrogen in Bone: Anti-Osteoporotic Effects (1993)

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Risk of Diabetes Mellitus Associated With Atypical Antipsychotic Use Among Patients With Bipolar Disorder (2006)

Development or exacerbation of diabetes mellitus is associated with antipsychotic use in bipolar patients. Metabolic complications are a major issue in patients receiving antipsychotic therapy. Thus, the propensity of an antipsychotic to induce diabetes should be a consideration when selecting an agent for patients with bipolar disorder.
Dynamics of susceptibility and transmissibility of the live, attenuated, candidate vaccines dengue-1 PDK13, dengue-3 PGMK30F3, and dengue-4 PDK48 after oral infection in Aedes aegypti. (1999)

Dengue-1 virus PDK13 and isolates from vaccinees (dengue-1 Ib1 and dengue-1 Ib 10), dengue-3 PGMK30F3, and dengue-4 PDK48 were studied for their abilities to infect, disseminate, and replicate in Aedes aegypti mosquitoes by the oral route. In general, infection and dissemination rates were poorer for the vaccine compared with the parent viruses. The transmissibility was also lower for dengue-1 PDK13 than the parent virus, whereas it was not detected for isolates from vaccinees and dengue-3 PGMK30F3. Transmissibility of dengue-4 PDK48 was not determined because no dissemination occurred. Replication rates of vaccine strains were also found to be less efficient than the parent viruses. These imply that vaccination with the candidate vaccine is safe. Moreover, vector attenuation of vaccine viruses was consistent with its phenotypic markers of attenuation, which remained stable after a mosquito passage or after human and mosquito passage.
Doenca de Fahr – a Proposito de um Caso Clinico (2014)

A doença de Fahr é uma doença neuropsiquiátrica rara caracterizada por calcificação dos gânglios basais, podendo-se manifestar por alterações do movimento, demência e doenças afectivas. O diagnóstico é feito através de métodos imagiológicos (TAC ou RMN CE). É apresentado um caso clínico de doença de Fahr, em que os primeiros sintomas de doença foram alterações do humor.
Trends in the search for bioactive microbial metabolites (1992)

Bioactive microbial metabolites are attracting increasing attention as useful agents for medicine, veterinary medicine, agriculture, and as unique biochemical tools. A review of the current trends in the discovery of new metabolites shows that the number of active compounds with non-antibiotic type of activity has increased, resulting in an expansion of the variety of bioactivity of microbial metabolites. Factors that contribute to the increased rate of discovery include: development of new methods for activity measurement, exploitation of novel groups of microorganisms as sources of active compounds, new directions for chemical modification, and incorporation of newer knowledge of biotechnology into screening systems. To exemplify this, typical screening methods, and chemical and biological properties of several bioactive compounds obtained by these methods are discussed.
Joint request mapping and response routing for geo-distributed cloud services (2013)

Many cloud services are running on geographically distributed datacenters for better reliability and performance. We consider the emerging problem of joint request mapping and response routing with distributed datacenters in this paper. We formulate the problem as a general workload management optimization. A utility function is used to capture various performance goals, and the location diversity of electricity and bandwidth costs are realistically modeled. To solve the large-scale optimization, we develop a distributed algorithm based on the alternating direction method of multipliers (ADMM). Following a decomposition-coordination approach, our algorithm allows for a parallel implementation in a datacenter where each server solves a small sub-problem. The solutions are coordinated to find an optimal solution to the global problem. Our algorithm converges to near optimum within tens of iterations, and is insensitive to step sizes. We empirically evaluate our algorithm based on real-world workload traces and latency measurements, and demonstrate its effectiveness compared to conventional methods.
Palindromic units from E. coli as binding sites for a chromoid‐associated protein (1986)

Several hundred copies of a highly conserved extragenic palindromic sequence, 20-40 nucleotides long, exist along the chromosome of E. coli and S. typhimurium. These have been defined as palindromic units (PU) or repetitive extragenic palindromes (REP). No general function for PUs has been identified. In the present work, we provide data showing that a protein associated with a chromoid extract of E. coli protects PU DNA against exonuclease III digestion. This provides the first experimental evidence that PU constitutes binding sites for a chromoid-associated protein. This result supports the hypothesis that PUs could play a role in the structure of the bacterial chromoid.