gebhart/patrepeval huggingface dataset:
Ku-band helicopter satellite communications for on scene disaster information transmission (2005)

Helicopter videoing is one of the most popular methods to acquire on scene information for disaster management and relief. We developed an on scene disaster information transmission system using Ku-band helicopter satellite communications. The main obstacle for a helicopter satellite communication link is the interruption by the rotating blades of the helicopter. To overcome the blade interruption, we use a time diversity transmission technique at the forward link and a blade-synchronized transmission technique at the return link. Furthermore, onboard position estimation is done for the on scene videoing pictures. Some primary field experiments have been done with satisfactory results.
Dynamically Redefinable Character SETS-D.R.C.S. (1980)

D. R. C. S. is potentially the most powerful and cost effective editorial addition to the Teletext and Viewdata services of all the refinements that have been discussed and proposed at various committees on these new public services.
Novel Routes to Chiral 2-Alkoxy-5-/6- methoxycarbonylmethylidenepyrrolidines/-piperidines (2004)

We report the results of a study aimed at the diastereoselective synthesis of chiral 2-alkoxy-5-/6-methoxycarbonylmethylidenepyrrolidines/-piperidines by condensation of chiral amines onto omega-oxo alkynoates and omega-oxo beta-keto esters.
Preparation and electrochemical characterization of (100 − x)(0.7Li2S·0.3P2S5)·xLiBr glass–ceramic electrolytes (2013)

Glass and glass–ceramic electrolytes of (100 − x)(0.7Li2S·0.3P2S5)·xLiBr (x = 0, 5, 10, 12.5, 15 and 20) (mol %) were synthesized by mechanical milling and subsequent heat treatment. Glass powders with no crystal phase were obtained by mechanical milling. The conductivities of the glasses increased concomitantly with increasing LiBr content. The conductivity at x = 20 was 3.1 × 10−4 S cm−1 at room temperature. The Li7P3S11 crystal with high Li ion conductivity was precipitated in the glass–ceramics obtained by heat treatment. LiBr crystal was also precipitated in the glass–ceramics containing LiBr as a starting material. The glass–ceramic at x = 10 showed conductivity of 6.4 × 10−3 S cm−1. It increased to 8.4 × 10−3 S cm−1 by control of the milling period to prepare the precursor glass. 90(0.7Li2S·0.3P2S5)·10LiBr glass–ceramic with high Li ion conductivity had a wide electrochemical window of 10 V. An all-solid-state lithium secondary battery using 90(0.7Li2S·0.3P2S5)·10LiBr glass–ceramic as an electrolyte was charged and discharged successfully.
Synthesis and characterization of mRNA cap analogs containing phosphorothioate substitutions that bind tightly to eIF4E and are resistant to the decapping pyrophosphatase DcpS (2008)

Analogs of the mRNA cap are widely employed to study processes involved in mRNA metabolism as well as being useful in biotechnology and medicinal applications. Here we describe synthesis of six dinucleotide cap analogs bearing a single phosphorothioate modification at either the alpha, beta, or gamma position of the 5',5'-triphosphate chain. Three of them were also modified with methyl groups at the 2'-O position of 7-methylguanosine to produce anti-reverse cap analogs (ARCAs). Due to the presence of stereogenic P centers in the phosphorothioate moieties, each analog was obtained as a mixture of two diastereomers, D1 and D2. The mixtures were resolved by RP HPLC, providing 12 different compounds. Fluorescence quenching experiments were employed to determine the association constant (K(AS)) for complexes of the new analogs with eIF4E. We found that phosphorothioate modifications generally stabilized the complex between eIF4E and the cap analog. The most strongly bound phosphorothioate analog (the D1 isomer of the beta-substituted analog m(7)Gpp(S)pG) was characterized by a K(AS) that was more than fourfold higher than that of its unmodified counterpart (m(7)GpppG). All analogs modified in the gamma position were resistant to hydrolysis by the scavenger decapping pyrophosphatase DcpS from both human and Caenorhabditis elegans sources. The absolute configurations of the diastereomers D1 and D2 of analogs modified at the alpha position (i.e., m(7)Gppp(S)G and m(2) (7,2'-O )Gppp(S)G) were established as S(P) and R(P) , respectively, using enzymatic digestion and correlation with the S(P) and R(P) diastereomers of guanosine 5'-O-(1-thiodiphosphate) (GDPalphaS). The analogs resistant to DcpS act as potent inhibitors of in vitro protein synthesis in rabbit reticulocyte lysates.
Telomerase and the Cellular Lifespan: Implications for the Aging Process (2000)

The aging process has multiple causes. However, there is now substantial evidence consistent with the hypothesis that (i) all normal mammalian somatic cells have a finite capacity to replicate and (ii) that gradual cell turnover throughout the lifespan of a mammal eventually exhausts this finite capacity. This results in a gradual accumulation of senescent (irreversibly post-mitotic) cells with increasing age. These cells display a radically different phenotype to their growing counterparts, which has the potential to compromise tissue function. Perhaps the best evidence for this is seen in Werner's syndrome, a rare genetic disease, in which patients display most of the features of accelerated aging, together with a profoundly compromised replicative lifespan in certain tissue lineages. Several classes of human cells are now known to count divisions by monitoring the progressive attrition of chromosomal ends (telomeres), leading to the activation of a p53-p21waf-dependent G1 checkpoint. Ectopic expression of telomerase has been shown to prevent senescence in several cell types and offers the potential for interventions in the aging process based on tissue engineering, gene therapy or homeografts. However, this telomere-driven senescence mechanism seems to be absent from rodents, which use telomere-independent means (perhaps based upon p14arf) to count divisions. Similar senescence pathways are now being reported in humans, and this, coupled with the demonstration of tissue-specific telomeric loss rates, has the potential to render strategies based on the use of telomerase dependent on the characteristics of the target tissue. Werner's syndrome may provide strong clues regarding the potential limitations and prospects of such future treatments.
Similarity between fluorescein-specific T-cell receptor and antibody in chemical details of antigen recognition. (1992)

A computer-generated model of the single-chain variable V alpha V beta fragment of the RFL3.8 T-cell receptor (TCR) specific for fluorescein served as a starting point for mutagenesis aimed at identification of its antigen-contacting residues. Selected backbone segments of the model representing regions of prominent sequence similarity between antibodies and TCRs were least-squares superimposed onto the corresponding segments of the crystallographically resolved 4-4-20 antibody complexed with its antigen, fluorescein. The superimposition placed the antibody-bound fluorescein molecule close to a cavity on the surface of the TCR model formed by the complementarity-determining region (CDR) loops. Some of the TCR cavity forming loops displayed sequence motifs related to canonical CDR loops previously found in antibodies. Six putative amino acid contacts were identified and single-chain TCRs with mutations at each of these positions were expressed in Escherichia coli, purified, refolded, and assayed for fluorescein binding. Five of the six mutations resulted in a loss of detectable binding. These RFL3.8 antigen combining site residues are distributed among the beta 3, alpha 1, and alpha 2 CDR loops and show striking chemical similarity to the known fluorescein contact residues on 4-4-20. Thus, antibodies and TCRs are similar both in their overall architecture and in the chemical details of specific antigen recognition.
Controlled Radical Polymerization of Methacrylic Monomers in the Presence of a Bis(ortho-chelated) Arylnickel(II) Complex and Different Activated Alkyl Halides (1996)

A novel class of homogeneous nickel(II) catalysts, i.e [Ni{o,o‘(CH2NMe2)2C6H3}Br], denoted as Ni(NCN‘)Br, is reported to mediate in the presence of activated alkyl halides, e.g., CCl4 or α-halocarbonyl compounds, a well-controlled radical polymerization of methacrylic monomers [methyl and n-butyl methacrylate), (MMA, n-BuMA)] at rather low temperatures (<100 °C). The number-average molecular weight of the polymer gradually increased with the monomer conversion and was inversely proportional to the initiator concentration of alkyl halides. The molecular weight distribution (MWD) remained very narrow during the whole course of the polymerization (MWD < 1.3). All the experimental data including a successful block copolymerization (n-BuMA-b-MMA) experiment were in agreement with a living polymerization process, and remarkably enough, poly(methyl methacrylate) (PMMA) with molecular weight up to at least 105 g/mol was synthesized in a controlled fashion. Increased thermal stability of the PMMA is a further indication of the high regioselectivity and the virtually absence of termination reactions. Owing to the compatibility of the Ni(II) complexes toward water, extension to aqueous suspension polymerization was attempted successfully as attested by the promising preliminary results. Indications on the mechanism let us suggest that the reactive alkyl halide or the corresponding growing chain end is reversibly activated/deactivated by single electron transfer together with the halogen transfer.
Pretransplant Infusion of Mesenchymal Stem Cells Prolongs the Survival of a Semiallogeneic Heart Transplant through the Generation of Regulatory T Cells (2008)

In this study, we investigated whether mesenchymal stem cells (MSC) had immunomodulatory properties in solid organ allotransplantation, using a semiallogeneic heart transplant mouse model, and studied the mechanism(s) underlying MSC tolerogenic effects. Either single (portal vein, day -7) or double (portal vein, day -7 and tail vein, day -1) pretransplant infusions of donor-derived B6C3 MSC in B6 recipients induced a profound T cell hyporesponsiveness and prolonged B6C3 cardiac allograft survival. The protolerogenic effect was abrogated when donor-derived MSC were injected together with B6C3 hematopoietic stem cells (HSC), suggesting that HSC negatively impact MSC immunomodulatory properties. Both the induction (pretransplant) and the maintenance phase (>100 days posttransplant) of donor-derived MSC-induced tolerance were associated with CD4(+)CD25(+)Foxp3(+) Treg expansion and impaired anti-donor Th1 activity. MSC-induced regulatory T cells (Treg) were donor-specific since adoptive transfer of splenocytes from tolerant mice prevented the rejection of fully MHC-mismatched donor-specific secondary allografts but not of third-party grafts. In addition, infusion of recipient-derived B6 MSC tolerized a semiallogeneic B6C3 cardiac allograft, but not a fully MHC-mismatched BALB/c graft, and expanded Treg. A double i.v. pretransplant infusion of recipient-derived MSC had the same tolerogenic effect as the combined intraportal/i.v. MSC infusions, which makes the tolerogenic protocol applicable in a clinical setting. In contrast, single MSC infusions given either peritransplant or 1 day after transplant were less effective. Altogether these findings indicate that MSC immunomodulatory properties require HSC removal, partial sharing of MHC Ags between the donor and the recipient and pretransplant infusion, and are associated with expansion of donor-specific Treg.
Efficiency of viral DNA synthesis during infection of permissive and nonpermissive cells with vif-negative human immunodeficiency virus type 1 (1993)

The rate-limiting steps in infection by human immunodeficiency virus type 1 (HIV-1) deficient in the viral infectivity factor, Vif, are unknown. As a measurement of completion of the early stages of the HIV-1 life cycle, the levels of viral DNA were examined by polymerase chain reaction amplification during infection by vif-positive and vif-negative viruses of MT-2 and H9 cells, in which vif is required for HIV-1 replication. Viral DNA was detected within hours of infection by both viruses, but the accumulation of vif-negative virus DNA was impeded in terms of both extent and kinetics. Inefficient viral DNA synthesis correlated with restricted replication of the vif-negative virus. Increasing the input dose of vif-negative virus increased viral DNA levels within 24 h of infection but failed to overcome the block to subsequent DNA synthesis and productive infection. Infection of C8166 cells, in which vif function is dispensable, resulted in efficient DNA synthesis by vif-positive and vif-negative viruses. We conclude that one defect in the replication of vif-negative HIV-1 in nonpermissive cells occurs prior to or during viral DNA synthesis and may reflect processes required for efficient nucleocapsid internalization or activation of reverse transcription.
Dye—ligand affinity chromatography on continuous beds (1995)

Continuous beds derivatized with three triazine dyes (Cibacron Blue 3G-A, Pricon Red HE-3B and Pricon Red H-3B) were used for chromatographic purification of dehydrogenases from yeast enzyme concentrate. All three columns, which were prepared by a very simple and cost-effective method, provided strong binding of glucose-6-phosphate dehydrogenase and lactate dehydrogenase. The Cibacron Blue 3G-A column showed high affinity for alcohol dehydrogenase. Under the same conditions, the Pricon Red HE-3B column showed a lower affinity and the Pricon Red H-3B column showed none. The adsorbed dehydrogenases were eluted specifically from the columns in high yields (71-113% by desorption with the coenzymes NADP, NADH and NAD respectively). Non-specific binding of human serum albumin and transferrin to these columns was also investigated. Enzyme assays and analyses by capillary electrophoresis showed that the continuous beds derivatized with triazine dyes gave a high degree of purification.
Deoxyribonucleic acid plasmids in yeasts (1989)

Description de tous les plasmides a DNA isoles et caracterises chez les levures et mecanismes aboutissant a la persistance ce ces elements extrachromosomiques (139 references). L'analyse de ces elements a abouti a de nouvelles donnees sur l'amplification de domaines chromosomiques specifiques, la proteolyse des proteines secretees et le controle de la replication du DNA
Intranasal organic dust exposure-induced airway adaptation response marked by persistent lung inflammation and pathology in mice (2009)

Organic dust exposure in agricultural environments results in an inflammatory response that attenuates over time, but repetitive exposures can result in chronic respiratory disease. Animal models to study these mechanisms are limited. This study investigated the effects of single vs. repetitive dust-induced airway inflammation in mice by intranasal exposure method. Mice were exposed to swine facility dust extract (DE) or saline once and once daily for 1 and 2 wk. Dust exposure resulted in increased bronchoalveolar lavage fluid neutrophils and macrophages after single and repetitive exposures. Lavage fluid TNFalpha, IL-6, keratinocyte chemoattractant, and macrophage inflammatory protein-2 were significantly increased after single and repetitive dust exposures, but were dampened in 2-wk dust-exposed mice compared with single exposure. Dust exposure induced PKCalpha and -epsilon activation in isolated tracheal epithelial cells but were dampened with repetitive exposures. Ex vivo stimulation of alveolar macrophages from 2-wk animals demonstrated reduced cytokine responsiveness and phagocytic ability. Significant lung pathology occurred with development of mixed mononuclear cellular aggregates (T and B lymphocytes, phagocytes) after repetitive dust exposure, a novel observation. Airway hyperresponsiveness to methacholine occurred after single dust exposure but resolved after 2 wk. Collectively, intranasal exposure to DE results in significant lung inflammatory and pathological responses marked by a modulated innate immune response to single and repetitive dust exposures that is associated with PKC activity.
Novel Boron Nitride Hollow Nanoribbons (2008)

Novel BN hollow nanoribbons (BNHNRs) were fabricated by a simple ZnS nanoribbon templating method. Such BNHNRs have a distinct structure and show unique optical properties, as demonstrated from Raman, Fourier transform infrared spectroscopy, UV-vis spectroscopy, and cathodoluminescence spectroscopy, when compared with other forms of BN nanostructures. With high crystallinity, the BNHNRs exhibit an extraordinary ultraviolet CL emission at 5.33 eV. Such a property is highly advantageous for optoelectronic applications, particularly in the ultraviolet region, such as blue lasing and light emitting diodes. This templating method has also been extended to synthesize other hollow nanostructures such as boron carbonitride. This study represents a new methodology for fabricating hollow nanostructures with defined crystallinity and unique optical properties.
A close ultrastructural relationship between sulfated proteoglycans and AA amyloid fibrils. (1987)

Two cationic reagents, Ruthenium red (RR) and Cuprolinic blue (CB), were used to assess the morphologic and structural relationship between sulfated proteoglycans and AA amyloid fibrils in amyloidotic spleen and liver, and in isolated fibril preparations. Amyloidotic tissue fixed in the presence of RR showed RR granules, measuring 15 to 25 nm in diameter, over areas of electrondense fibrils. In isolated fibril preparations, RR granules were specifically localized on amyloid fibrils. Amyloidotic tissue fixed in the presence of CB at 0.1 M and 0.7 M MgCl2 showed both granule and filamentous (50 to 90 nm in length) staining only over areas of amyloid fibrils. This same staining localization was also seen in isolated fibril preparations. The RR and CB granules and filaments, are believed to represent proteoglycan monomers with the glycosaminoglycan chains collapsed onto the protein core. The persistent CB staining at 0.7 M magnesium chloride suggested that highly sulfated proteoglycans were present. The glycosaminoglycan moiety has previously been identified as heparin/heparan sulphate. The intimate structural relationship between sulfated proteoglycans and AA amyloid fibrils, both in situ and in isolated fibril preparations, further suggests that these highly negatively charged molecules may have an important role in the pathogenesis of amyloidosis. Several pathogenetic scenarios are suggested.
Pathologic Features of Experimentally Induced Chlamydial Infection in Dogs (1972)

SUMMARY Inoculation of 6 dogs with the chlamydial agent of ovine polyarthritis resulted in infection in 5 dogs that was pathologically characterized by focal hepatitis. Lymphoid and reticuloendothelial hyperplasia of spleen and lymph node was seen in 3 of 5 dogs inoculated by the intravenous route. Two of these dogs had acute leptomeningitis. One dog developed fibrino-purulent polyarthritis, along with hyperplastic synovitis, bursitis, and tendovaginitis. In 4 dogs killed 4 to 7 days after inoculation, the chlamydial agent was consistently isolated from liver and spleen, but was less consistently isolated from other organs.
Static Conductance and Scaling Theory of Localization in One Dimension (1981)

It is shown that the Ohmic dc conductance of a one-dimensional system equals to either ${G}_{p}=(\frac{{e}^{2}}{\ensuremath{\pi}\ensuremath{\hbar}})T$ or ${G}_{c}=\frac{(\frac{{e}^{2}}{\ensuremath{\pi}\ensuremath{\hbar}})T}{(1\ensuremath{-}T)}$, depending on whether the system is connected to a perfect one-dimensional conductor or to a classical wire (behaving as a current source), respectively. The parameter $\ensuremath{\beta}\ensuremath{\equiv}\frac{d〈\mathrm{ln}{g}^{\ensuremath{'}}〉}{d\mathrm{ln}L}$, where $L$ is the length of the system, depends only on $〈\mathrm{ln}{g}^{\ensuremath{'}}〉$, so that $\ensuremath{\beta}=〈\mathrm{ln}{g}^{\ensuremath{'}}〉$, with ${g}^{\ensuremath{'}}=(\frac{\ensuremath{\pi}\ensuremath{\hbar}}{{e}^{2}}){G}_{p}$.
Low-cost compact diffuse speckle contrast flowmeter using small laser diode and bare charge-coupled-device (2016)

We report a low-cost compact diffuse speckle contrast flowmeter (DSCF) consisting of a small laser diode and a bare charge-coupled-device (CCD) chip, which can be used for contact measurements of blood flow variations in relatively deep tissues (up to ∼8 mm). Measurements of large flow variations by the contact DSCF probe are compared to a noncontact CCD-based diffuse speckle contrast spectroscopy and a standard contact diffuse correlation spectroscopy in tissue phantoms and a human forearm. Bland–Altman analysis shows no significant bias with good limits of agreement among these measurements: 96.5%±2.2% (94.4% to 100.0%) in phantom experiments and 92.8% in the forearm test. The relatively lower limit of agreement observed in the in vivo measurements (92.8%) is likely due to heterogeneous reactive responses of blood flow in different regions/volumes of the forearm tissues measured by different probes. The low-cost compact DSCF device holds great potential to be broadly used for continuous and longitudinal monitoring of blood flow alterations in ischemic/hypoxic tissues, which are usually associated with various vascular diseases.
The phenotypic spectrum of SCN8A encephalopathy (2015)

SCN8A encephalopathy presents in infancy with multiple seizure types including focal seizures and spasms in some cases. Outcome is often poor and includes hypotonia and movement disorders. The majority of mutations arise de novo, although we observed a single case of somatic mosaicism in an unaffected parent.
Geometrical cloning of 3D objects via simultaneous registration of multiple range images (1997)

Presents a method for the reconstruction of real-world objects from multiple range images. One major contribution of our approach is the simultaneous registration of all range images acquired from different scanner views. Thus, registration errors are not accumulated, and it is even possible to reconstruct large objects from an arbitrary number of small range images. The registration process is based on a least-squares approach where a distance metric between the overlapping range images is minimized. A resolution hierarchy accelerates the registration substantially. After registration, a volumetric model of the object is carved out. This step is based on the idea that no part of the object can lie between the measured surface and the camera of the scanner. With the marching cube algorithm, a polygonal representation is generated. The accuracy of this polygonal mesh is improved by moving the vertices of the mesh on to the surface implicitly defined by the registered range images.