gebhart/patrepeval huggingface dataset:
Laser-Induced Breakdown Spectroscopy of Liquids: Aqueous Solutions of Nickel and Chlorinated Hydrocarbons (1998)

Spectrochemical analyses of aqueous solutions containing nickel or the chlorinated hydrocarbons (CHCs) C 2 Cl 4 , CCl 4 , CHCl 3 , and C 2 HCl 3 were performed with the use of laser-induced breakdown spectroscopy. A Nd:YAG laser operating at 60 mJ/pulse was focused onto the surface of the liquid. Elemental line intensities were monitored in the laser-produced plume as a function of analyte concentration to determine detection limits. The limits of detection for nickel in water were 36.4 ± 5.4 mgAL and 18.0 ± 3.8 mg/L for laser irradiation at 1.06 μm and 355 nm, respectively. Ablation of pure CHCs at 355 nm produced extremely intense plasma emissions that primarily consisted of spectroscopic features attributed to CN, C 3 , H, N, and Cl. The spectra were structurally identical for all the CHCs except for differences in the intensities of various emission lines. With the use of emission from neutral atomic chlorine as an identifier for CHC contamination of water, no detectable traces of these elements were observed in saturated aqueous solutions. The detection limits for the CHCs were well above the saturation limits of CHC in water.
Inhibitions of Histamine Release and Prostaglandin E2 Synthesis by Mangosteen, a Thai Medicinal Plant. (2002)

The fruit hull of mangosteen, Garcinia mangostana L. has been used as a Thai indigenous medicine for many years. However, its mechanism of action as a medicine has not been elucidated. The present study was undertaken to examine the effects of mangosteen extracts (100% ethanol, 70% ethanol, 40% ethanol and water) on histamine release and prostaglandin E2 synthesis. We found that the 40% ethanol extract of mangosteen inhibited IgE-mediated histamine release from RBL-2H3 cells with greater potency than the water extract of Rubus suavissimus that has been used as an anti-allergy crude drug in Japan. All extracts of mangosteen potently inhibited A23187-induced prostaglandin E2 synthesis in C6 rat glioma cells, while the water extract of Rubus suavissimus had no effect. The 40% ethanol extract of mangosteen inhibited the prostaglandin E2 synthesis in a concentration-dependent manner with relatively lower concentrations than the histamine release. In addition, passive cutaneous anaphylaxis (PCA) reactions in rats were significantly inhibited by this ethanol extract as well as by the water extract of Rubus suavissimus. These results suggest that the 40% ethanol extract of mangosteen has potent inhibitory activities of both histamine release and prostaglandin E2 synthesis.
Microbubble‐mediated sonoporation for highly efficient transfection of recalcitrant human B‐ cell lines (2014)

Sonoporation has not been widely explored as a strategy for the transfection of heterologous genes into notoriously difficult-to-transfect mammalian cell lines such as B cells. This technology utilizes ultrasound to create transient pores in the cell membrane, thus allowing the uptake of extraneous DNA into eukaryotic and prokaryotic cells, which is further enhanced by cationic microbubbles. This study investigates the use of sonoporation to deliver a plasmid encoding green fluorescent protein (GFP) into three human B-cell lines (Ramos, Raji, Daudi). A higher transfection efficiency (TE) of >42% was achieved using sonoporation compared with <3% TE using the conventional lipofectamine method for Ramos cells. Upon further antibiotic selection of the transfected population for two weeks, we successfully enriched a stable population of GFP-positive Ramos cells (>70%). Using the same strategy, Raji and Daudi B cells were also successfully transfected and enriched to 67 and 99% GFP-positive cells, respectively. Here, we present sonoporation as a feasible non-viral strategy for stable and highly efficient heterologous transfection of recalcitrant B-cell lines. This is the first demonstration of a non-viral method yielding transfection efficiencies significantly higher (42%) than the best reported values of electroporation (30%) for Ramos B-cell lines.
Korea's Move to e-Procurement (2004)

In 1997 the Korean government began&#13;\n reforming its notoriously complicated, nontransparent,&#13;\n corrupt public procurement system, introducing e-procurement&#13;\n to exploit the country's well-developed information and&#13;\n communications infrastructure. Through extensive business&#13;\n process reengineering and information strategy planning, the&#13;\n Public Procurement Service-the agency formerly responsible&#13;\n for buying government goods and services-has been&#13;\n transformed into a one-stop information center.&#13;\n E-procurement has generated numerous benefits, including:&#13;\n Enhanced transparency and public trust-by reducing contacts&#13;\n between officials and suppliers and by sharing information&#13;\n between government agencies and the public. Increased&#13;\n managerial efficiency-by achieving economies of scale in&#13;\n procurement, with an estimated $2.5 billion a year in&#13;\n savings from the $26 million investment. This note analyzes&#13;\n how Korea achieved these outcomes, the lessons of its&#13;\n experience, and the challenges that remain for its&#13;\n e-procurement system.
Metabolic imaging with gallium-68- and indium-111-labeled low-density lipoprotein. (1991)

Low-density lipoprotein (LDL) labeled with either gallium-68 (68Ga) or indium-111 (111In) was evaluated as a potential PET or SPECT radiopharmaceutical for determination of hepatic lipoprotein metabolism in rabbits. Gallium-68 or 111In was linked to LDL via diethylenetriaminepentaacetic acid (DTPA) with a 25-70% radiochemical yield. Studies in vivo that compared 68Ga- or 111In-DTPA-LDL with dilactitol-[125I]-tyramine LDL and 131I-LDL showed that both 68Ga- and 111In-labeled LDL behaved as residualizing radiotracers. Localization of radioactivity within the liver of normal rabbits was visualized clearly with [68Ga]DTPA-LDL by PET and with [111In]DTPA-LDL by gamma scintigraphy. Significant differences were observed in hepatic uptake of normal compared with hypercholesterolemic rabbits in which low-capacity LDL receptor-mediated catabolism was saturated. Gallium-68 and 111In-DTPA-LDL are attractive radiopharmaceuticals for noninvasive delineation of tissue LDL metabolism under normal and pathophysiologic conditions.
Planck's blackbody radiation law: Presentation in different domains and determination of the related dimensional constants (2009)

In this paper the Planck function is derived in the frequency domain using the method of oscillators. It is also presented in the wavelength domain and in the wave number domain. The latter is mainly used in spectroscopy for studying absorption and emission by gases. Also the power law of Stephan and Boltzmann is derived for these various domains. It is shown that this power law is generally independent of the domain in which the Planck function is presented. A formula for the filtered spectrum is also given and expressed in the sense of the power law of Stefan and Boltzmann. Furthermore, based on Wien's displacement relationship, it is argued that the wavelength at which the maximum of the Planck function presented in the wavelength domain occurs differs from that of the maxima of the other domains by a factor of 1.76. As Planck determined his elementary quantum of action, eventually called the Planck constant, and the Boltzmann constant using Wien's displacement relationship formulated for the wavelength domain, it is shown that the values of these fundamental constants are not affected by the choice of domain in which the Planck function is presented. Finally, the origin of the Planck constant is discussed.
Sequence of 1060 3'-terminal nucleotides of poliovirus RNA as determined by a modification of the dideoxynucleotide method. (1980)

The dideoxynucleotide method for sequencing DNA developed by Sanger et al. [Sanger, F., Nicklen, S. & Coulson, A. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467] was modified to allow sequence analysis of poliovirus RNA without recourse to cloning. Our method involves reverse transcription of poliovirus RNA followed by cDNA-dependent DNA synthesis in the presence of unlabeled dNTPs and 2',3'-dideoxynucleoside triphosphates, with Escherichia coli DNA polymerase I (Klenow) used to catalyze the reaction. DNA synthesis is primed by 5'-32P-labeled RNase T1- or RNase A-resistant oligonucleotides generated from poliovirus RNA. The sequence of 1060 nucleotides preceding the 3'-terminal poly(A) is presented. Based on the position of termination codons we propose that viral translation terminates at nucleotide -562.
Disease specific protein corona (2015)

It is now well accepted that upon their entrance into the biological environments, the surface of nanomaterials would be covered by various biomacromolecules (<i>e.g.,</i> proteins and lipids). The absorption of these biomolecules, so called ‘protein corona’, onto the surface of (nano)biomaterials confers them a new ‘biological identity’. Although the formation of protein coronas on the surface of nanoparticles has been widely investigated, there are few reports on the effect of various diseases on the biological identity of nanoparticles. As the type of diseases may tremendously changes the composition of the protein source (<i>e.g.,</i> human plasma/serum), one can expect that amount and composition of associated proteins in the corona composition may be varied, in disease type manner. Here, we show that corona coated silica and polystyrene nanoparticles (after interaction with in the plasma of the healthy individuals) could induce unfolding of fibrinogen, which promotes release of the inflammatory cytokines. However, no considerable releases of inflammatory cytokines were observed for corona coated graphene sheets. In contrast, the obtained corona coated silica and polystyrene nanoparticles from the hypofibrinogenemia patients could not induce inflammatory cytokine release where graphene sheets do. Therefore, one can expect that disease-specific protein coronas can provide a novel approach for applying nanomedicine to personalized medicine, improving diagnosis and treatment of different diseases tailored to the specific conditions and circumstances.
TAL effector-DNA specificity (2010)

TAL effectors are important virulence factors of bacterial plant pathogenic Xanthomonas, which infect a wide variety of plants including valuable crops like pepper, rice, and citrus. TAL proteins are translocated via the bacterial type III secretion system into host cells and induce transcription of plant genes by binding to target gene promoters. Members of the TAL effector family differ mainly in their central domain of tandemly arranged repeats of typically 34 amino acids each with hypervariable di-amino acids at positions 12 and 13. We recently showed that target DNA-recognition specificity of TAL effectors is encoded in a modular and clearly predictable mode. The repeats of TAL effectors feature a surprising one repeat-to-one-bp correlation with different repeat types exhibiting a different DNA base pair specificity. Accordingly, we predicted DNA specificities of TAL effectors and generated artificial TAL proteins with novel DNA recognition specificities. We describe here novel artificial TALs and discuss implications for the DNA recognition specificity. The unique TAL-DNA binding domain allows design of proteins with potentially any given DNA recognition specificity enabling many uses for biotechnology.
A distributed search service for peer-to-peer file sharing in mobile applications (2003)

In this paper, we present the concept of passive distributed indexing, a general-purpose distributed search service for mobile file sharing applications, which is based on peer-to-peer technology. The service enables resource-effective searching for files distributed across mobile devices based on simple queries. Building blocks of PDI constitute local broadcast transmission of query- and response messages, together with caching of query results at every device participating in PDI. Based on these building blocks, the need for flooding the entire network with query messages can be eliminated for most application. In extensive simulation studies, we demonstrate the performance of PDI. Because the requirements of a typical mobile file sharing application are not known-or even do not exist at all-we study the performance of PDI for different system environments and application requirements. We show that due to the flexible design PDI can be employed for several kinds of applications.
Immunoelectrophoretic characterization of Sendai virus antigens. (1977)

Antigens extracted from Sendai virus by solubilization with Triton X-100 (Tx). Tween-20 (Tw) or Triton X-100 and SDS (Tx-SDS) were analysed by rocket, crossed and tandem immunoelectrophoresis. The migration of these antigens through 1% agarose supplemented with guinea pig anti-viral envelope protein anti-serum shows that Tx and Tw antigens consist of three fractions differing in their quantitative ratios, while Tx-SDS exhibits five different fractions. The method proved to be useful for the identification of anti-host antibodies in immune antisera by means of an antigen extracted from the chorio-allantoic membrane of embryonated hen eggs.
Inhibitory Fcγ Receptor Engagement Drives Adjuvant and Anti-Tumor Activities of Agonistic CD40 Antibodies (2011)

CD40, a member of the tumor necrosis factor receptor (TNFR) superfamily, is expressed on antigen-presenting cells (APCs) and is essential for immune activation. Although agonistic CD40 antibodies have been developed for immunotherapy, their clinical efficacy has been limited. We have found that coengagement of the Fc domain of agonistic CD40 monoclonal antibodies (mAbs) with the inhibitory Fcγ receptor FcγRIIB is required for immune activation. Direct comparison of mAbs to CD40 enhanced for activating FcγR binding, hence capable of cytotoxicity, or for inhibitory FcγRIIB binding, revealed that enhancing FcγRIIB binding conferred immunostimulatory activity and considerably greater anti-tumor responses. This unexpected requirement for FcγRIIB in enhancing CD40-mediated immune activation has direct implications for the design of agonistic antibodies to TNFR as therapeutics.
Permeability of Rheumatoid and Normal Human Synovium to Specific Plasma Proteins (1981)

A method is described for the determination of the permeability of the blood-joint barrier to specific plasma proteins, using the ratio of protein concentration in synovial fluid to that in plasma. The inadequacy of the ratio per se as a direct index of permeability is discussed. Permeabilities are evaluated for the normal and rheumatoid human knee. Permeability increases in the rhematoid knee by approximately 6 times for the rheumatoid knee by approximately 6 times for albumin and over 40 times for macroglobulins. The effect of protein molecular dimensions upon permeability is analyzed. Permeability shows less dependence upon solute dimensions in the rheumatoid knee than in the normal knee, i.e., molecular selectivity is reduced. From these data and synovial morphology, a two-membrane model of the blood-joint barrier is developed. The relative contribution of the component intimal and endothelial layers to the total barrier is found to depend upon solute dimensions.
Realizable Hamiltonians for universal adiabatic quantum computers (2008)

It has been established that local lattice spin Hamiltonians can be used for universal adiabatic quantum computation. However, the two-local model Hamiltonians used in these proofs are general and hence do not limit the types of interactions required between spins. To address this concern, the present paper provides two simple model Hamiltonians that are of practical interest to experimentalists working toward the realization of a universal adiabatic quantum computer. The model Hamiltonians presented are the simplest known quantum-Merlin-Arthur-complete (QMA-complete) two-local Hamiltonians. The two-local Ising model with one-local transverse field which has been realized using an array of technologies, is perhaps the simplest quantum spin model but is unlikely to be universal for adiabatic quantum computation. We demonstrate that this model can be rendered universal and QMA-complete by adding a tunable two-local transverse ${\ensuremath{\sigma}}^{x}{\ensuremath{\sigma}}^{x}$ coupling. We also show the universality and QMA-completeness of spin models with only one-local ${\ensuremath{\sigma}}^{z}$ and ${\ensuremath{\sigma}}^{x}$ fields and two-local ${\ensuremath{\sigma}}^{z}{\ensuremath{\sigma}}^{x}$ interactions.
NAAG peptidase inhibitors block cognitive deficit induced by MK-801 and motor activation induced by d-amphetamine in animal models of schizophrenia (2012)

The most widely validated animal models of the positive, negative and cognitive symptoms of schizophrenia involve administration of d-amphetamine or the open channel NMDA receptor blockers, dizocilpine (MK-801), phencyclidine (PCP) and ketamine. The drug ZJ43 potently inhibits glutamate carboxypeptidase II (GCPII), an enzyme that inactivates the peptide transmitter N-acetylaspartylglutamate (NAAG) and reduces positive and negative behaviors induced by PCP in several of these models. NAAG is an agonist at the metabotropic glutamate receptor 3 (mGluR3). Polymorphisms in this receptor have been associated with expression of schizophrenia. This study aimed to determine whether two different NAAG peptidase inhibitors are effective in dopamine models, whether their efficacy was eliminated in GCPII knockout mice and whether the efficacy of these inhibitors extended to MK-801-induced cognitive deficits as assessed using the novel object recognition test. ZJ43 blocked motor activation when given before or after d-amphetamine treatment. (R,S)-2-phosphono-methylpentanedioic acid (2-PMPA), another potent NAAG peptidase inhibitor, also reduced motor activation induced by PCP or d-amphetamine. 2-PMPA was not effective in GCPII knockout mice. ZJ43 and 2-PMPA also blocked MK-801-induced deficits in novel object recognition when given before, but not after, the acquisition trial. The group II mGluR antagonist LY341495 blocked the effects of NAAG peptidase inhibition in these studies. 2-PMPA was more potent than ZJ43 in a test of NAAG peptidase inhibition in vivo. By bridging the dopamine and glutamate theories of schizophrenia with two structurally different NAAG peptidase inhibitors and demonstrating their efficacy in blocking MK-801-induced memory deficits, these data advance the concept that NAAG peptidase inhibition represents a potentially novel antipsychotic therapy.
Women Litigators in Search of a Care-Oriented Judicial System (1995)

Discourage litigation.Persuade your neighbors to compromise whenever you can.Point out to them how the nominal winner is often a real loserin fees, expenses and waste of time.As a peacemake, the lawyer has a superior opportunity of being a good man.Never stir up litigation.A worse man can scarcely be found than one who does this.Who can be more nearly a fiend than he who habitually overhauls the register of deeds in search of defects in titles, whereupon to stir up strife and put money in his pocket?A moral tone ought to be enforced in the profession which would drive such men out of it.
Conditional Independence in Statistical Theory (1979)

Summary Some simple heuristic properties of conditional independence are shown to form a conceptual framework for much of the theory of statistical inference. This framework is illustrated by an examination of the rôle of conditional independence in several diverse areas of the field of statistics. Topics covered include sufficiency and ancillarity, parameter identification, causal inference, prediction sufficiency, data selection mechanisms, invariant statistical models and a subjectivist approach to model-building.
Fexofenadine HCl Immediate Release Tablets: In vitro Characterization and Evaluation of Excipients (2013)

Fexofenadine HCl immediate release tablets were designed to increase the dissolution rate by using superdisintegrants. Different formulations of Fexofenadine HCl were prepared by direct compression method. These formulations were evaluated for hardness, thickness, friability, weight variation, disintegration time, and in vitro dissolution study. The drug release from the formulations were studied according to USP specification (USP paddle method at 50 rpm for 60 minutes) maintaining the temperature to 37°C. Sodium starch glycolate, cross carmellose sodium, crospovidone (kollidon CL), ludiflash and xanthan gum were used in 3%, 6% and 8% concentrations as superdisintegrants. Thus, the ratio of superdisintegrants was changed whereas all the other excipients as well as the active drug (Fexofenadine HCl) remained same in every formulation. Here, 0.001N HCl was used as dissolution medium according to USP and absorbances were determined by using UV spectrophotometer at 217 nm. The F-3 and F-6 formulation prepared by 8% of Sodium starch glycolate and 8% of Cross carmellose sodium showed 99.99% drug release within 30 minutes and 45 minutes, respectively. The disintegration times of F-3 and F-6 formulation were within 9 seconds. The interactions between drug and excipients were characterized by FTIR spectroscopic study. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14483 Bangladesh Pharmaceutical Journal 16(1): 1-9, 2013
Henry VIII: An Annotated Bibliography. (1989)

Journal Article Linda McJ. Micheli, comp. Henry VIII: An Annotated Bibliography. Get access Henry VIII: An Annotated Bibliography. Compiled by Linda Mcj . Micheli. New York and London: Garland, 1988. Pp. xxxii + 444. $62.00 cloth. John D. Cox John D. Cox Search for other works by this author on: Oxford Academic Google Scholar Shakespeare Quarterly, Volume 40, Issue 4, Winter 1989, Pages 509–511, https://doi.org/10.2307/2870620 Published: 01 December 1989
Measurement of Atmospheric Ozone by Cavity Ring-down Spectroscopy (2011)

Ozone plays a key role in both the Earth's radiative budget and photochemistry. Accurate, robust analytical techniques for measuring its atmospheric abundance are of critical importance. Cavity ring-down spectroscopy has been successfully used for sensitive and accurate measurements of many atmospheric species. However, this technique has not been used for atmospheric measurements of ozone, because the strongest ozone absorption bands occur in the ultraviolet spectral region, where Rayleigh and Mie scattering cause significant cavity losses and dielectric mirror reflectivities are limited. Here, we describe a compact instrument that measures O3 by chemical conversion to NO2 in excess NO, with subsequent detection by cavity ring-down spectroscopy. This method provides a simple, accurate, and high-precision measurement of atmospheric ozone. The instrument consists of two channels. The sum of NO2 and converted O3 (defined as Ox) is measured in the first channel, while NO2 alone is measured in the second channel. NO2 is directly detected in each channel by cavity ring-down spectroscopy with a laser diode light source at 404 nm. The limit of detection for O3 is 26 pptv (2 sigma precision) at 1 s time resolution. The accuracy of the measurement is ±2.2%, with the largest uncertainty being the effective NO2 absorption cross-section. The linear dynamic range of the instrument has been verified from the detection limit to above 200 ppbv (r2>99.99%). The observed precision on signal (2 sigma) with 41 ppbv O3 is 130 pptv in 1 s. Comparison of this instrument to UV absorbance instruments for ambient O3 concentrations shows linear agreement (r2=99.1%) with slope of 1.012±0.002.