gebhart/patrepeval huggingface dataset:
A sketch retrieval method for full color image database-query by visual example (2003)

Gives a basic idea and its fundamental algorithms of the visual interface for image database systems. The QVE (Query by Visual Example) accepts a sketch roughly drawn by a user to retrieve the original image and the similar images. The system evaluates the similarity between the rough sketch, i.e. a visual example, and each of the image data in the database automatically. The QVE interface is implemented and examined on an experimental electronic art gallery called ART MUSEUM. This paper also gives some experimental results and a current evaluation. The algorithms are quite effective for content based image retrieval.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Xilinx FPGA design in a group environment using VHDL and synthesis tools (1996)

The use of VHDL and synthesis tools has been shown to be well suited to achieving the objectives in a group design environment. The expressiveness of VHDL lends itself to group discussion and with synthesis, the outcome is correct by construction and can be verified rapidly in a true system environment built around a Xilinx FPGA. Not surprisingly however since this year has seen the first full run of students through the course there have been problems. Students in their second year are not sufficiently mature to accept that fighting the CAD system and winning is a necessary part of becoming a successful designer and so they complain continually about the apparent dumbness of Autologic, its slowness and its seemingly obscure error messages. The complaints about the VHDL compiler run in a similar vein. Perhaps one day CAD tools of this complexity really will become quicker and less hostile. However, what this year's group cannot appreciate is how much more productive and successful they have been than have several years of preceding cohorts using traditional design methods with schematic capture. More help will of course be available on how to write VHDL to achieve more efficient synthesis as more experience is gained by staff of the seemingly infinite set of traps waiting to catch the novice designer.
Suppression of proliferation of human epidermal keratinocytes by 1,25‐dihydroxyvitamin D3 Analysis of its effect on psoriatic lesion and of its mechanism using human keratinocytes in culture (1991)

Psoriasis is a genetically determined chronic disease of the skin, and an accelerated proliferation of epidermal keratinocytes is one of the pathophysiological characteristics of psoriatic lesion. Recently, it was reported that topical administration of 1,25-dihydroxyvitamin D3 was effective for psoriasis. Our study was done to investigate the effect of 1,25-(OH)2-D3 on cell kinetics on human epidermal keratinocytes as a possible mechanism of its effect on psoriasis. After 24-h application of 1 microgram g-1 1,25-(OH)2-D3 ointment to psoriatic lesion, the number of mitotic keratinocytes decreased. When the cultured human keratinocytes were exposed to 10(-8) mol l-1 1,25-(OH)2-D3 for more than 9 days, inhibition of cell proliferation was noted. DNA distribution analysis by flow cytometry showed a decrease in cells in the S phase, and increase in 2c cells. This indicates blockade of the cell cycle in the G1 phase. The cell cycle time was not extended as a result of 1,25-(OH)2-D3-treatment.
Targeting the ensemble of heterogeneous tau oligomers in cells: A novel small molecule screening platform for tauopathies (2019)

Our biosensor technology is broadly applicable to other areas of tauopathy therapeutic development. These biosensors can be readily modified for different isoforms of tau, specific post-translational modifications, and familial Alzheimer's disease-associated mutations. We are eager to explore tau interactions with chaperone proteins, monitor cross-reactivity with other intrinsically disordered proteins, and target seeded oligomer pathology.
Spectroscopic investigation of energetic atoms in a DC hydrogen glow discharge (1990)

The analysis of the Doppler profile of the Balmer Halpha line in the cathode sheath and negative glow of a hydrogen glow discharge shows an important concentration of anisotropic energetic atoms. This translational energy is regarded as mainly resulting from reactions including ions accelerated by the cathode sheath electric field. The mechanisms involved are gas-phase charge-exchange collisions producing fast atoms moving toward the cathode and simultaneous neutralisation and backscattering of the ions at the cathode surface leading to energetic atoms moving in the other direction. Quantitative analysis shows that most of the emission of the fast atoms results from excitation directly produced by these ion impact reactions.
Human lymph node morphology as a function of age and site. (1980)

Cervical, axillary, cubital, inguinal, popliteal, and mesenteric lymph nodes from subjects of various ages who had died a sudden death were examined histologically. Care was taken to establish by morphometry the proportional distribution in lymph node cross-sections of cortical, paracortical, and medullary areas. In addition, numbers and surface areas of cross-sectioned germinal centres were registered. Important differences related to age and anatomical site of lymph nodes were established by this survey. Germinal centre formation, particularly evident in infants and children, less so in young adults, and often absent in ageing individuals, was most impressive in lymph nodes normally exposed to antigenic stimulation (mesenteric and cervical lymph nodes). Paracortical and medullary areas exhibited a slight but gradual reduction with advancing age. Replacement of lymphatic parenchyma by fat tissue (lipomatous atrophy) was a characteristic of more peripheral lymph nodes usually subjected to little antigenic stimulation, that is, cubital, axillary, and popliteal nodes. It should be emphasised that both age-related and regional differences have to be taken into account in a meaningful functional interpretation of lymph node morphology.
G-protein Palmitoyltransferase Activity Is Enriched in Plasma Membranes (1996)

Heterotrimeric G proteins are covalently modified by lipids. Myristoylation of G-protein alpha subunits and prenylation of gamma subunits are stable modifications. In contrast, palmitoylation of alpha subunits is dynamic and thus has the potential for regulating protein function. Indeed, receptor activation of Gs increases palmitate turnover on the alpha subunit, presumably by stimulating deacylation. The enzymes that catalyze reversible palmitoylation of G-protein alpha subunits have not been characterized. Here we report the identification of a palmitoyl-CoA:protein S-palmitoyltransferase activity that acylates G-protein alpha subunits in vitro. Palmitoyltransferase activity is membrane-associated and requires detergent for solubilization. The preferred G-protein substrate for the enzyme activity is the alpha subunit in the context of the heterotrimer. Both myristoylated and nonmyristoylated G-protein alpha subunits are recognized as substrates. The palmitoyltransferase activity demonstrates a modest preference for palmitoyl-CoA over other fatty acyl-CoA substrates. Palmitoyltransferase activity is high in plasma membrane and present at low or undetectable levels in Golgi, endoplasmic reticulum, and mitochondria of rat liver. The subcellular localization of this enzyme activity is consistent with a role for regulated cycles of acylation and deacylation accompanying activation of G-protein signal transduction pathways.
Secure Deniable Authentication Protocol Based on ElGamal Cryptography (2008)

In 2006, Shao et al. showed that Wang et al.'s deniable authentication protocol based on ElGamal cryptography is insecure to a person-in-the-middle attack; that is, in the whole process, the receiver cannot be aware of the existence of this adversary as well as the modification of the messages. Furthermore, they also presented a modification of Wang et al.'s protocol to overcome the security flaw. However, the current paper demonstrates that Shao et al.'s improved deniable authentication protocol is susceptible to a malicious receiver's impersonation attack. To mitigate this security breach, we propose an improved deniable authentication protocol based on ElGamal cryptography.
Deposition of a Thin Film of TiOx from a Titanium Metal Target as Novel Blocking Layers at Conducting Glass/TiO2 Interfaces in Ionic Liquid Mesoscopic TiO2 Dye-Sensitized Solar Cells (2006)

In dye-sensitized TiO2 solar cells, charge recombination processes at interfaces between fluorine-doped tin oxide (FTO), TiO2, dye, and electrolyte play an important role in limiting the photon-to-electron conversion efficiency. From this point of view, a high work function material such as titanium deposited by sputtering on FTO has been investigated as an effective blocking layer for preventing electron leakage from FTO without influencing electron injection. X-ray photoelectron spectroscopy analysis indicates that different species of Ti (Ti4+, Ti3+, Ti2+, and a small amount of Ti0) exist on FTO. Electrochemical and photoelectrochemical measurements reveal that thin films of titanium species, expressed as TiOx, work as a compact blocking layer between FTO and TiO2 nanocrystaline film, improving Voc and the fill factor, finally giving a better conversion efficiency for dye-sensitized TiO2 solar cells with ionic liquid electrolytes.
Mode of Nitric Oxide Delivery Affects Antibacterial Action (2019)

Nitric oxide (NO) is a broad-spectrum antibacterial agent, making it an attractive alternative to traditional antibiotics for treating infections. To date, a direct comparison of the antibacterial activity of gaseous NO (gNO) versus water-soluble NO-releasing biopolymers has not been reported. In this study, the bactericidal action of NO-releasing chitosan oligosaccharides was compared to gNO treatment against cystic fibrosis-relevant Gram-positive and Gram-negative bacteria. A NO exposure chamber was constructed to enable the dosing of bacteria with gNO at concentrations up to 800 ppm under both aerobic and anaerobic conditions. Bacteria viability, solution properties (i.e., pH, NO concentration), and toxicity to mammalian cells were monitored to ensure a thorough understanding of bactericidal action and reproducibility for each delivery method. The NO-releasing chitosan oligosaccharides required significantly lower NO doses relative to gNO therapy to elicit antibacterial action against <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> under both aerobic and anaerobic conditions. Reduced NO doses required for bacteria eradication using water-soluble NO-releasing chitosan were attributed to the release of NO in solution, removing the need to transfer from gas to liquid phase and the associated long diffusion distances of gNO treatment.
Glutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures (1995)

In vitro ischemia models have utilized oxygen, or oxygen and glucose deprivation to simulate ischemic neuronal injury. Combined oxygen and glucose deprivation can induce neuronal damage which is in part mediated through NMDA receptors. Severe oxygen deprivation alone however can cause neuronal injury which is not NMDA mediated. We tested the hypothesis that NMDA, or non-NMDA receptor mediated mechanisms may predominate, to induce neuronal injury following severe oxygen deprivation depending on the presence of glucose. We found that NMDA receptor blockade using dizocilpine (MK-801), DL-2-amino-5-phosphonovaleric acid (APV), or CGS 19755, was highly effective in reducing CA1 injury in organotypic hippocampal cultures, caused by complete oxygen and glucose deprivation. Complete oxygen deprivation alone however, caused CA1 neuronal injury which was not diminished using NMDA receptor blockade alone with MK-801 or APV, or in combination with AMPA/kainate receptor blockade using 6-cyano-7-dinitroquinoxalone-2,3-dione (CNQX). Neuronal protective strategies which act primarily through non-glutamate dependent mechanisms, including hypothermia, low chloride and calcium, and the free radical scavenger, alpha-phenyl-tert-butyl nitrone (PBN), provided neuronal protection against complete oxygen, as well as combined oxygen/glucose deprivation. Raising the pH using Hepes buffer during complete oxygen deprivation did not result in neuronal protection by NMDA receptor blockade. Partial oxygen deprivation alone, partial oxygen deprivation combined with glucose deprivation, glucose deprivation alone, and also glutamate exposure, all produced neuronal damage that was reduced by NMDA receptor blockade. The presence of glucose during complete oxygen deprivation appears to prevent glutamate receptor blockade from reducing neuronal injury in organotypic hippocampal cultures.
Isolation of human immunodeficiency virus from a Japanese hemophilia B patient with AIDS. (1987)

Human immunodeficiency virus (HIV) was isolated from a Japanese hemophilia B patient with AIDS. This isolate, HIV[GUN-1], was infectious to several mature T-cell lines. Proteins with apparent molecular weights of 160, 55 and 25 kilodaltons were detected. Restriction enzyme cleavage patterns of the proviral genome indicated that HIV[GUN-1] is related to but clearly different from HTLV-III or ARV-2.
Nucleophilic reactivity constants toward methyl iodide and trans-dichlorodi(pyridine)platinum(II) (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleophilic reactivity constants toward methyl iodide and trans-dichlorodi(pyridine)platinum(II)Ralph G. Pearson, Harold R. Sobel, and Jon SongstadCite this: J. Am. Chem. Soc. 1968, 90, 2, 319–326Publication Date (Print):January 1, 1968Publication History Published online1 May 2002Published inissue 1 January 1968https://pubs.acs.org/doi/10.1021/ja01004a021https://doi.org/10.1021/ja01004a021research-articleACS PublicationsRequest reuse permissionsArticle Views2288Altmetric-Citations403LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Towards beautification of freehand sketches using suggestions (2009)

Beautification of freehand sketches is integral for building robust sketch understanding systems and sketch-based interfaces for CAD. Many of the current methods for beautification do not consider some important information implied in the sketches such as spatial relationships (geometric constraints) between primitives. In addition, as the freehand input is ambiguous in nature, correctly interpreting the visual scene the user has in mind is a difficult problem. To this extent, we present our ongoing work, a suggestive interface for constraint-driven beautification of freehand sketches which provides multiple interpretations of the freehand input, from which the user can choose the intended result. A preliminary user study has been conducted to evaluate the effectiveness of the proposed method.
Preparation and characterization of HLA-A*0201 tetramer loaded with IE-1316-324 antigenic peptide of human cytomegalovirus. (2006)

Major histocompatibility complex (MHC) class I tetramer technology has become the central technique for analyzing antigen-specific CD8(+) T cell responses and it has been widely used to explore the differentiation and formation of memory CD8(+) T cells. Previously, a simplified and efficient procedure for preparing high quality HLA-A*0201 tetramers has been established in our lab and the tetramers loaded with HCMV peptide pp65(495-503) has been successfully applied to investigate HCMV-specific CD8(+) T cells in Chinese populations. Using similar procedure we reported here the construction of HLA-A*0201 tetramer loaded with another dominant epitope derived from immediate early (IE)-1(316-324) (VLEETSVML, VLE) of HCMV (A2-VLE) and characterization of this tetramer. After A2-VLE monomer was prepared and purified, its tetramer was then formed at a yield of 83%. The optimized amount of A2-VLE tetramer for staining 100 microl whole blood was 0.5 microg with incubation at 4 degrees C for 1 h. Furthermore, the dissociation constant of the tetramer binding to the specific CD8(+) T cells of one HLA-A2(+) donor was estimated to be 32.7 nmol/L, which is markedly higher than that of MHC monomer. The construction of A2-VLE tetramer provides an alternative choice for investigating HCMV-specific CD8(+) T cell responses and will deepen our understanding of the differentiation and formation of HCMV-specific memory CD8(+) T cells.
4-1BB Agonist Focuses CD8+ Tumor-Infiltrating T-Cell Growth into a Distinct Repertoire Capable of Tumor Recognition in Pancreatic Cancer (2017)

<b>Purpose:</b> Survival for pancreatic ductal adenocarcinoma (PDAC) patients is extremely poor and improved therapies are urgently needed. Tumor-infiltrating lymphocyte (TIL) adoptive cell therapy (ACT) has shown great promise in other tumor types, such as metastatic melanoma where overall response rates of 50% have been seen. Given this success and the evidence showing that T-cell presence positively correlates with overall survival in PDAC, we sought to enrich for CD8<sup>+</sup> TILs capable of autologous tumor recognition. In addition, we explored the phenotype and T-cell receptor repertoire of the CD8<sup>+</sup> TILs in the tumor microenvironment.<b>Experimental Design:</b> We used an agonistic 4-1BB mAb during the initial tumor fragment culture to provide 4-1BB costimulation and assessed changes in TIL growth, phenotype, repertoire, and antitumor function.<b>Results:</b> Increased CD8<sup>+</sup> TIL growth from PDAC tumors was achieved with the aid of an agonistic 4-1BB mAb. Expanded TILs were characterized by an activated but not terminally differentiated phenotype. Moreover, 4-1BB stimulation expanded a more clonal and distinct CD8<sup>+</sup> TIL repertoire than IL2 alone. TILs from both culture conditions displayed MHC class I-restricted recognition of autologous tumor targets.<b>Conclusions:</b> Costimulation with an anti-4-1BB mAb increases the feasibility of TIL therapy by producing greater numbers of these tumor-reactive T cells. These results suggest that TIL ACT for PDAC is a potential treatment avenue worth further investigation for a patient population in dire need of improved therapy. <i>Clin Cancer Res; 23(23); 7263-75. ©2017 AACR</i>.
Fluorinated poly(benzoxazole–imide)s (1994)

Abstract New poly(benzoxazole–imide)s have been prepared by polycondensation of bis(o‐aminophenol)s, such as 3,3′ dihydroxybenzidine or 2,2‐bis(3‐amino‐4‐hydroxyphenyl)‐hexafluoropropane, with diacid chlorides containing both imide and hexafluoroisopropylidene groups. The soluble polymer precursor resulting from the first step of polycondensation in N‐methylpyrrolidinone (NMP) at low temperature was processed into thin flexible films, which were then thermally treated to reach the fully cyclized structure of poly(benzoxazole–imide)s, as observed by infrared spectra. An alternative way to produce the cyclization was by heating at reflux the solution of polymer precursor in NMP. Thermal stability, glass transition temperature, dielectric constant and its dependence on relative humidity have been studied and compared with those of related polybenzoxazoles and other heterocyclic Polymers.
Phagocytosis of Microbes: Complexity in Action (2002)

The phagocytic response of innate immune cells such as macrophages is defined by the activation of complex signaling networks that are stimulated by microbial contact. Many individual proteins have been demonstrated to participate in phagocytosis, and the application of high-throughput tools has indicated that many more remain to be described. In this review, we examine this complexity and describe how during recognition, multiple receptors are simultaneously engaged to mediate internalization, activate microbial killing, and induce the production of inflammatory cytokines and chemokines. Many signaling molecules perform multiple functions during phagocytosis, and these molecules are likely to be key regulators of the process. Indeed, pathogenic microorganisms target many of these molecules in their attempts to evade destruction.
Practical selectivity estimation through adaptive sampling (1990)

Recently we have proposed an adaptive, random sampling algorithm for general query size estimation. In earlier work we analyzed the asymptotic efficiency and accuracy of the algorithm, in this paper we investigate its practicality as applied to selects and joins. First, we extend our previous analysis to provide significantly improved bounds on the amount of sampling necessary for a given level of accuracy. Next, we provide “sanity bounds” to deal with queries for which the underlying data is extremely skewed or the query result is very small. Finally, we report on the performance of the estimation algorithm as implemented in a host language on a commercial relational system. The results are encouraging, even with this loose coupling between the estimation algorithm and the DBMS.
Calcium Signaling in Neurons: Molecular Mechanisms and Cellular Consequences (1995)

Neuronal activity can lead to marked increases in the concentration of cytosolic calcium, which then functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calmodulin and stimulates the activity of a variety of enzymes, including calcium-calmodulin kinases and calcium-sensitive adenylate cyclases. These enzymes transduce the calcium signal and effect short-term biological responses, such as the modification of synaptic proteins and long-lasting neuronal responses that require changes in gene expression. Recent studies of calcium signal-transduction mechanisms have revealed that, depending on the route of entry into a neuron, calcium differentially affects processes that are central to the development and plasticity of the nervous system, including activity-dependent cell survival, modulation of synaptic strength, and calcium-mediated cell death.