gebhart/patrepeval huggingface dataset:
Measurements of a distributed file system (1991)

We analyzed the user-level file access patterns and caching behavior of the Sprite distributed file system. The first part of our analysis repeated a study done in 1985 of the: BSD UNIX file system. We found that file throughput has increased by a factor of 20 to an average of 8 Kbytes per second per active user over 10-minute intervals, and that the use of process migration for load sharing increased burst rates by another factor of six. Also, many more very large (multi-megabyte) files are in use today than in 1985. The second part of our analysis measured the behavior of Sprite's main-memory file caches. Client-level caches average about 7 Mbytes in size (about one-quarter to one-third of main memory) and filter out about 50% of the traffic between clients and servers. 35% of the remaining server traffic is caused by paging, even on workstations with large memories. We found that client cache consistency is needed to prevent stale data errors, but that it is not invoked often enough to degrade overall system performance.
The Changing Epidemiology of Meningococcal Disease in the United States, 1992–1996 (1999)

New meningococcal vaccines are undergoing clinical trials, and changes in the epidemiologic features of meningococcal disease will affect their use. Active laboratory-based, population-based US surveillance for meningococcal disease during 1992-1996 was used to project that 2400 cases of meningococcal disease occurred annually. Incidence was highest in infants; however, 32% of cases occurred in persons >/=30 years of age. Serogroup C caused 35% of cases; serogroup B, 32%; and serogroup Y, 26%. Increasing age (relative risk [RR], 1.01 per year), having an isolate obtained from blood (RR, 4.5), and serogroup C (RR, 1.6) were associated with increased case fatality. Among serogroup B isolates, the most commonly expressed serosubtype was P1.15; 68% of isolates expressed 1 of the 6 most common serosubtypes. Compared with cases occurring in previous years, recent cases are more likely to be caused by serogroup Y and to occur among older age groups. Ongoing surveillance is necessary to determine the stability of serogroup and serosubtype distribution.
Linking primary health care researchers in South Australia: a network strategy, SARNet (2004)

In response to the changing definitions of primary care delivery there is a growing need for research to be practitioner-driven and multidisciplinary. This paper addresses the strategies used to enhance multidisciplinary participation and capacity building in research in the South Australian Primary Health Care Research Network (SARNet). This network has 198 members currently. Membership is strongly multidisciplinary and spans all levels of research expertise. The services offered by the network are member-driven and include competitive bursary funding for research and evaluation skills development, writing groups, training events, access to web-based resources and information, special interest groups, and email alerts. Potential future directions include a database of members’ research interests and an online discussion forum or list server. The strategies, challenges and future impact of the network are discussed.
Experimental Demonstration of Dynamic Bandwidth Allocation Using a MEMS-Actuated Bandwidth-Tunable Microdisk Resonator Filter (2007)

A novel bandwidth-tunable optical filter based on a microelectromechanical-system-actuated microdisk resonator is utilized for dynamic bandwidth allocation. The filter bandwidth can be dynamically adjusted by voltage tuning the gap spacing between the two waveguides and microdisk. Three key applications of dynamic bandwidth allocation are demonstrated experimentally. Matched optical filtering is achieved on 5-Gb/s nonreturn-to-zero (NRZ) signals with error-free ( 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-9</sup> bit-error-rate) data transmission. Reconfigurable channel banding of three 2.5-Gb/s NRZ signals is demonstrated by either routing a single or a group of data channels. Wavelength demultiplexing of three channels under the worst-case scenario shows 14.5-dB suppression with error-free operation.
One-component bimetallic aluminium(salen)-based catalysts for cyclic carbonate synthesis and their immobilization (2010)

The development of one-component, bimetallic μ-oxoaluminium(salen) complexes as highly active catalysts for the synthesis of cyclic carbonates from terminal epoxides is described. The resulting homogeneous catalysts are used in batch reactions at room temperature and one atmosphere pressure. The catalysts have also been immobilized onto various support materials and used in either batch reactions or gas-phase flow reactions with ethylene and propylene oxides. Catalyst lifetime, deactivation and reactivation have been studied in both batch and flow reactions, and it has been shown that of the impurities present in power station flue gas, only sulfur trioxide deactivates the catalyst and at the concentrations of sulfur trioxide present in flue gas, this deactivation would require more than one years exposure of the catalyst to flue gas.
A learning theory approach to non-interactive database privacy (2008)

We demonstrate that, ignoring computational constraints, it is possible to release privacy-preserving databases that are useful for all queries over a discretized domain from any given concept class with polynomial VC-dimension. We show a new lower bound for releasing databases that are useful for halfspace queries over a continuous domain. Despite this, we give a privacy-preserving polynomial time algorithm that releases information useful for all halfspace queries, for a slightly relaxed definition of usefulness. Inspired by learning theory, we introduce a new notion of data privacy, which we call distributional privacy, and show that it is strictly stronger than the prevailing privacy notion, differential privacy.
Growth characteristics of the chimeric Japanese encephalitis virus vaccine candidate, ChimeriVax-JE (YF/JE SA14--14--2), in Culex tritaeniorhynchus, Aedes albopictus, and Aedes aegypti mosquitoes. (2000)

The Japanese encephalitis (JE) virus vaccine candidate, ChimeriVax-JE, which consists of a yellow fever (YF) 17D virus backbone containing the prM and E genes from the JE vaccine strain JE SA14--14--2, exhibits restricted replication in non-human primates, producing only a low-level viremia following peripheral inoculation. Although this reduces the likelihood that hematophagous insects could become infected by feeding on a vaccinated host, it is prudent to investigate the replication kinetics of the vaccine virus in mosquito species that are known to vector the viruses from which the chimera is derived. In this study ChimeriVax-JE virus was compared to its parent viruses, as well as to wild-type JE virus, for its ability to replicate in Culex tritaeniorhynchus, Aedes albopictus, and Aedes aegypti mosquitoes. Individual mosquitoes were exposed to the viruses by oral ingestion of a virus-laden blood meal or by intrathoracic (IT) virus inoculation. ChimeriVax-JE virus did not replicate following ingestion by any of the three mosquito species. Additionally, replication was not detected after IT inoculation of ChimeriVax-JE in the primary JE virus vector, Cx. tritaeniorhynchus. ChimeriVax-JE exhibited moderate growth following IT inoculation into Ae. aegypti and Ae. albopictus, reaching titers of 3.6-5.0 log(10) PFU/mosquito. There was no change in the virus genotype associated with replication in mosquitoes. Similar results were observed in mosquitoes of all three species that were IT inoculated or had orally ingested the YF 17D vaccine virus. In contrast, all mosquitoes either IT inoculated with or orally fed wild-type and vaccine JE viruses became infected, reaching maximum titers of 5.4-7.3 log(10) PFU/mosquito. These results indicate that ChimeriVax-JE virus is restricted in its ability to infect and replicate in these mosquito vectors. The low viremia caused by ChimeriVax-JE in primates and poor infectivity for mosquitoes are safeguards against secondary spread of the vaccine virus.
Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives (1994)

Superoxide (O2-.), nitric oxide (.NO), and their reaction product peroxynitrite (ONOO-) have all been shown to independently exert toxic target molecule reactions. Because these reactive species are often generated in excess during diverse inflammatory and other pathologic circumstances, we assessed the influence of .NO on membrane lipid peroxidation induced by O2-., H2O2, and .OH derived from xanthine oxidase (XO) and by ONOO-. Experimental conditions in lipid oxidation systems were adjusted to yield different rates of delivery of .NO, relative to rates of O2-. and H2O2 generation, by infusion of either .NO or via .NO released from S-nitroso-N-acetylpenicillamine or S-nitrosoglutathione. Peroxidation of phosphatidylcholine liposomes was assessed by formation of thiobarbituric acid-reactive products and by liquid chromatography-mass spectrometry. Liposomes exposed to XO-derived reactive species in the presence of .NO exhibited both stimulation and inhibition of lipid peroxidation, depending on the ratio of the rates of reactive oxygen species production and .NO introduction into reaction systems. Nitric oxide alone did not induce lipid peroxidation. Linolenic acid emulsions peroxidized by XO-derived reactive species showed similar dose-dependent regulation of lipid peroxidation by .NO. Mass spectral analysis of oxidation products showed formation of nitrito-, nitro-, nitrosoperoxo-, and/or nitrated lipid oxidation adducts, demonstrating that .NO serves as a potent terminator of radical chain propagation reactions. Electron spin resonance (ESR) analysis of incubation mixtures provided no evidence for formation of paramagnetic iron-lipid-nitric oxide complexes in reaction systems. Peroxynitrite-dependent lipid peroxidation, which predominantly occurs by metal-independent mechanisms, was also inhibited by .NO. Peroxynitrite-mediated benzoate hydroxylation was partially inhibited by .NO, inferring reaction between .NO and ONOOH. It is concluded that .NO can both stimulate O2-./H2O2/.OH-induced lipid oxidation and mediate oxidant-protective reactions in membranes at higher rates of .NO production, with the prooxidant versus antioxidant outcome critically dependent on relative concentrations of individual reactive species. Prooxidant reactions of .NO will occur after O2-. reaction with .NO to yield potent secondary oxidants such as ONOO- and the antioxidant effects of .NO a consequence of direct reaction with alkoxyl and peroxyl radical intermediates during lipid peroxidation, thus terminating lipid radical chain propagation reactions.
Automated detection of all kinds of violations at a street intersection using real time individual vehicle tracking (2003)

In this paper we introduce a system that detects all kinds of violations at a street intersection such as red light running, speed violation, stop line violation and lane violation by tracking individual vehicles. Two cameras are used for detecting violations. One is an analog camera for real-time tracking and the other is a digital camera for license plate reading. This system is connected to the traffic signal system controller and monitors the red, arrow, yellow and green phases of an approach. Two loops in the road are used to detect vehicle approach and speed. The system takes pictures of all vehicles passing a second loop and tracks the vehicles until they go out a street intersection. Traveling trajectory, approach lane, traffic signal and speed are used to decide whether the vehicle violates or not. The registration numbers and eight images of the violating vehicle are transmitted with an exclusive line to a central system at the metropolitan police agency.
Novel folded protein domains generated by combinatorial shuffling of polypeptide segments (2000)

It has been proposed that the architecture of protein domains has evolved by the combinatorial assembly and/or exchange of smaller polypeptide segments. To investigate this proposal, we fused DNA encoding the N-terminal half of a beta-barrel domain (from cold shock protein CspA) with fragmented genomic Escherichia coli DNA and cloned the repertoire of chimeric polypeptides for display on filamentous bacteriophage. Phage displaying folded polypeptides were selected by proteolysis; in most cases the protease-resistant chimeric polypeptides comprised genomic segments in their natural reading frames. Although the genomic segments appeared to have no sequence homologies with CspA, one of the originating proteins had the same fold as CspA, but another had a different fold. Four of the chimeric proteins were expressed as soluble polypeptides; they formed monomers and exhibited cooperative unfolding. Indeed, one of the chimeric proteins contained a set of very slowly exchanging amides and proved more stable than CspA itself. These results indicate that native-like proteins can be generated directly by combinatorial segment assembly from nonhomologous proteins, with implications for theories of the evolution of new protein folds, as well as providing a means of creating novel domains and architectures in vitro.
Human epicardial cell-conditioned medium contains HGF/IgG complexes that phosphorylate RYK and protect against vascular injury (2015)

Early treatment with HGF/IgG complexes after myocardial ischaemia with reperfusion may rescue tissue through vasoprotection conferred by c-Met and RYK signalling.
Demonstration on protein A of two distinct immunoglobulin-binding sites and their role in the mitogenic activity of Staphylococcus aureus Cowan I on human B cells. (1982)

Abstract The immunoglobulin-binding capacity of Staphylococcal protein A (SpA) and its role in the triggering of B cell proliferation induced by SpA-containing Staphylococcus aureus Cowan first strain (Cowan Staph) were investigated. Monoclonal lymphocytes from 34% of patients with surface IgM (slgM)-bearing B cell chronic lymphocytic leukemia (CLL) were found capable of binding to SpA. A similar proportion of monoclonal serum IgM proteins also showed the ability to react with SpA, whereas the remaining proportion of either slgM-bearing B cell CLL or monoclonal serum IgM proteins did not reveal any SpA-reactivity. Monomeric subunits as well as F(ab’)2µ fragments obtained from SpA-reactive monoclonal IgM proteins retained SpA-binding capacity, even though it was lower than that of native 19S IgM molecules. The SpA-reacting structure present on IgM molecules was snared by human polyclonal IgG and human F(ab’)2γ and F(ab’)2µ fragments but not by human Fcγ fragments or rabbit polyclonal IgG. The inactivation of tyrosil residues of SpA, which are essential for its Fcγ-binding ability, virtually abolished the capacity of SpA and Cowan Staph to react with rabbit IgG and human Fcγ fragments, but it did not alter the reactivity of either SpA or Cowan Staph with IgM molecules. The mitogenic activity of Cowan Staph on normal human B cells was inhibited by addition to the cell cultures of human polyclonal IgG or SpA-reactive monoclonal IgM proteins, but it was poorly influenced or not by influenced at all by addition of rabbit polyclonal IgG. Moreover, the inactivation of SpA-Fcγ-binding regions on Cowan Staph failed to affect the ability of these bacteria to induce proliferation of normal human B cells. In contrast, the Cowan Staph-induced proliferative response of surface IgG- (slgG) bearing B lymphocytes from a patient with CLL was abrogated either by addition to the cell cultures of very small amounts of rabbit polyclonal IgG or inactivation of the Fcγ-binding regions of SpA present on the bacterial cell wall. These data indicate the existence on SpA molecules of an IgM F(ab’)2-reactive site, distinct from the Fcγ-binding region. They also suggest that, even though the classical SpA-Fcγ interaction can represent a triggering signal for the Cowan Staph-induced proliferation of slgG-bearing B lymphocytes, the binding of SpA present on the bacterial cell wall to slgM is primarily responsible for the mitogenic activity of Cowan Staph on normal human B cells.
Specific Genetic Interference With Behavioral Rhythms in Drosophila by Expression of Inverted Repeats (2000)

Abstract We describe a new experimental technique that allows for a tissue-specific reduction of gene activity in the Drosophila nervous system. On the basis of the observation that certain gene functions can be ubiquitously blocked by injecting double-stranded RNA into Drosophila embryos, we employed a method to interfere with an individual gene function permanently in a predetermined cell type. This was achieved by the formation of an inverted-repeat RNA sequence in the tissue of interest under control of the GAL4/UAS binary expression system. As an example, we show that inverted-repeat-mediated interference with the period gene produces a hypomorphic period phenotype. A selective decrease of period RNA appears to be a component of the cellular response.
Thiobarbituric acid‐reactive malondialdehyde formation during superoxide‐dependent, iron‐catalyzed lipid peroxidation: Influence of peroxidation conditions (1989)

A systematic study of the influence of biological lipid peroxidation conditions on lipid hydroperoxide decomposition to thiobarbituric acid-reactive malondialdehyde is presented. A superoxide-dependent, iron-catalyzed peroxidation system was employed with xanthine oxidase plus hypoxanthine plus ferric iron-adenosine diphosphate complex as free radical generator. Purified cardiac membrane phospholipid (as liposomes) was the peroxidative target, and 15-hydroperoxy-eicosatetraenoic acid was used as a standard lipid hydroperoxide. Exposure of myocardial phospholipid to free radical generator at physiological pH (7.4) and temperature (37 degrees C) was found to support not only phospholipid peroxidation, but also rapid lipid hydroperoxide breakdown and consequent malondialdehyde formation during peroxidation. Under lipid peroxidation conditions, oxidative injury to the phospholipid polyunsaturated fatty acids required superoxide radical and ferric iron-adenosine diphosphate complex, whereas 37 degrees C temperature and trace iron were sufficient for lipid hydroperoxide decomposition to malondialdehyde. Harsh thiobarbituric acid-test conditions following peroxidation were not mandatory for either lipid hydroperoxide breakdown or thiobarbituric acid-reactive malondialdehyde formation. However, hydroperoxide decomposition that had begun in the peroxidation reaction could be completed during a subsequent thiobarbituric acid test in which no lipid autoxidation took place. Iron was more critical than heat in promoting the observed hydroperoxide decomposition to malondialdehyde during the lipid peroxidation reaction at 37 degrees C and pH 7.4. These data demonstrate that the radical generator, at physiological pH and temperature, serves a dual role as both initiator of membrane phospholipid peroxidation and promotor of lipid peroxide breakdown and thiobarbituric acid-reactive malondialdehyde formation.(ABSTRACT TRUNCATED AT 250 WORDS)
DNA Binding Specificity of the CCAAT-binding Factor CBF/NF-Y (1997)

CBF is a heterotrimeric protein that binds to DNA containing CCAAT motifs. Here we have analyzed interactions of recombinant CBF with DNA using hydroxyl radical footprinting and methylation interference assays. In the CBF-DNA complex, three separate DNA regions are protected from hydroxyl radical cleavage, one located over and immediately adjacent to the CCAAT motif itself and the other two located on both sides of the CCAAT motif. The methylation interference assay showed, however, that only in the CCAAT motif region methylation of bases was able to interfere with the formation of a CBF-DNA complex, suggesting that CBF makes sequence-specific contacts only in the CCAAT motif region. To further determine the specific DNA sequences necessary for CBF binding, we employed a polymerase chain reaction-mediated random binding site selection method. This analysis showed that CBF binding to DNA requires the CCAAT sequence and other specific sequences immediately flanking both ends of the CCAAT motif. We also showed that the nature of the flanking nucleotide sequences affects the affinity of CBF for DNA. Interestingly, most of the CCAAT motifs present in various higher eukaryotic promoters correspond to the CBF binding sites that were selected, consistent with the hypothesis that these motifs are binding sites for CBF and, hence, that CBF could regulate transcription of numerous eukaryotic genes.
Superoxide dismutase in Drosophila melanogaster: biochemical and structural characterization of allozyme variants. (1981)

Superoxide dismutase (SOD; superoxide:superoxide oxidoreductase, EC 1.15.1.1) is known to be polymorphic in many organisms; in Drosophila, the degree of polymorphism has a wide range of variation from locality to locality within a given species. We have thoroughly purified from D. melanogaster the two common electromorphs, SODS and SODF. These differ in properties such as isoelectric point, specific activity, rate constant, thermostability, and amino acid composition. The specific activity is three times greater in SODS than in SODF, but the latter is more thermostable. In strains from California, SODS differs from SODF by at least one amino acid substitution: lysine in SODS is replaced by either aspartic acid or asparagine in SODF. This difference is consistent with the electrophoretic mobility and isoelectric points of the two electromorphs. In strains from Africa, SODS and SODF differ by two amino acid substitutions (histidine and proline in SODS vs. serine and either glutamic acid or glutamine in SODF) in addition to the one distinguishing the California strains. Thus the SODF electromorphs from California and from Tunisia, in spite of their identical electrophoretic mobility, differ by at least two amino acid substitutions.
Insulin-Like Growth Factors I and II. Peptide, Messenger Ribonucleic Acid and Gene Structures, Serum, and Tissue Concentrations* (1989)

There is currently widespread interest in the IGFs (IGF-I and IGF-II) and their roles in the regulation of growth and differentiation of an ever increasing number of tissues are being reported. This selective review focused on the current state of our knowledge about the structure of mammalian IGFs and the multiple forms of mRNAs which arise from alternative splicing and promoter sites which arise from gene transcription. Current progress in the immunological measurement of the IGF is reviewed including different strategies for avoiding binding protein interference. The results of measurements of serum IGF-I and IGF-II in fetus and mother and at various stages of postnatal life are described. Existing knowledge of the concentration of these peptides in body fluids and tissues are considered. Last, an attempt is made to indicate circumstances in which the IGFs are exerting their actions in an autocrine/paracrine mode and when endocrine actions predominate. In the latter context it was concluded that an important role for GH action on skeletal tissues via hepatic production of IGF-I and endocrine action of IGF-I on growth cartilage is likely.