gebhart/patrepeval huggingface dataset:
Impact of obstructive sleep apnoea on left ventricular mass and global function (2005)

Obstructive sleep apnoea syndrome (OSAS) might be a cause of heart failure. The present study aimed to assess left ventricular mass and myocardial performance index (MPI) in OSAS patients. A total of 67 subjects without any cardiac or pulmonary disease, referred for evaluation of OSAS, had overnight polysomnography and echocardiography. According to apnoea-hypopnoea index (AHI), subjects were classified into three groups: mild OSAS (AHI: 5-14; n = 16), moderate OSAS (AHI: 15-29; n = 18), and severe OSAS (AHI: > or = 30; n = 33). Thickness of interventricular septum (IVS) and posterior wall (LVPW) were measured by M-mode, along with left ventricular mass (LVM) and LVM index (LVMI). Left ventricular MPI was calculated as (isovolumic contraction time+isovolumic relaxation time)/aortic ejection time by Döppler echocardiography. There were no differences in age or body mass index among the groups, but blood pressures were higher in severe OSAS compared with moderate and mild OSAS. In severe OSAS, thickness of IVS (11.2+/-1.1 mm), LVPW (11.4+/-0.9 mm), LVM (298.8+/-83.1 g) and LVMI (144.7+/-39.8 g x m(-2)) were higher than in moderate OSAS (10.9+/-1.3 mm; 10.8+/-0.9 mm; 287.3+/-74.6 g; 126.5+/-41.2 g x m(-2), respectively) and mild OSAS (9.9+/-0.9 mm; 9.8+/-0.8 mm; 225.6+/-84.3 g; 100.5+/-42.3 g x m(-2), respectively). In severe OSAS, MPI (0.64+/-0.14) was significantly higher than in mild OSAS (0.50+/-0.09), but not significantly higher than moderate OSAS (0.60+/-0.10). In conclusion, severe and moderate obstructive sleep apnoea syndrome patients had higher left ventricular mass and left ventricular mass index, and also left ventricular global dysfunction.
Metabolism of oral cephalothin and related cephalosporins in the rat (1967)

The fate of orally administered [(14)C]cephalothin has been studied in the rat. This antibiotic undergoes degradation in the gut followed by the subsequent absorption of a portion of the degradation products. About 50% of the administered radioactivity appears in the urine as a mixture of thienylacetylglycine, thienylacetamidoethanol and an unidentified polar metabolite. Evidence is presented indicating that thienylacetamidoethanol arises by the enzymic reduction of a metabolic intermediate, thienylacetamidoacetaldehyde. The metabolic fate of cephalothin is very similar to that of cephaloram reported earlier. The fate of [(14)C]cephaloridine and 7-phenoxy[1-(14)C]acetamidocephalosporin was also investigated. In addition to the expected metabolites, about 5% of the cephaloridine dose is absorbed unchanged. With 7-phenoxy[1-(14)C]acetamidocephalosporin, 15% of the dose is recovered in urine as deacetyl-7-phenoxy[1-(14)C]acetamidocephalosporin.
Identification of Attenuated Yersinia pseudotuberculosis Strains and Characterization of an Orogastric Infection in BALB/c Mice on Day 5 Postinfection by Signature-Tagged Mutagenesis (2001)

Yersinia pseudotuberculosis localizes to the distal ileum, cecum, and proximal colon of the gastrointestinal tract after oral infection. Using signature-tagged mutagenesis, we isolated 13 Y. pseudotuberculosis mutants that failed to survive in the cecum of mice after orogastric inoculation. Twelve of these mutants were also attenuated for replication in the spleen after intraperitoneal infection, whereas one strain, mutated the gene encoding invasin, replicated as well as wild-type bacteria in the spleen. Several mutations were in operons encoding components of the type III secretion system, including components involved in translocating Yop proteins into host cells. This indicates that one or more Yops may be necessary for survival in the gastrointestinal tract. Three mutants were defective in O-antigen biosynthesis; these mutants were also unable to invade epithelial cells as efficiently as wild-type Y. pseudotuberculosis. Several other mutations were in genes that had not previously been associated with growth in a host, including cls, ksgA, and sufl. In addition, using Y. pseudotuberculosis strains marked with signature tags, we counted the number of different bacterial clones that were present in the cecum, mesenteric lymph nodes, and spleen 5 days postinfection. We find barriers in the host animal that limit the number of bacteria that succeed in reaching and/or replicating in the mesenteric lymph nodes and spleen after breaching the gut mucosa.
Expression of insulin-like growth factor binding protein-1 and -2 genes through the perinatal period in the rat. (1993)

Insulin-like growth factor binding proteins (IGFBPs) are essential mediators of the bioavailability and biological effects of the IGFs. Liver expression of the rat (r) IGFBP-1 and rIGFBP-2 genes has been characterized between day 16 in utero (16 diu) and 16 days postnatally (+16 dpn). Run-on experiments showed transcriptional activity of the rIGFBP-1 and rIGFBP-2 genes at birth (B) to be 25 and 5 times that at 16 diu, respectively. After B, transcriptional activity of the rIGFBP-1 gene remained high (140% B at +6 dpn), but that of the rIGFBP-2 gene dropped to 70% B by +6 dpn. Northern blot analysis done simultaneously showed rIGFBP-1 messenger RNA (mRNA) levels to increase approximately 50-fold between 16 diu and B, whereas rIGFBP-2 mRNA increased only 5- to 10-fold. rIGFBP-1 mRNA levels decreased after birth, reaching about 20% B by +6 dpn; rIGFBP-2 mRNA, however, remained stable (about 80% B) at least up to +6 dpn. Parallel Western ligand blot and immunoblot analyses of serum rIGFBPs revealed rIGFBP-1 and rIGFBP-2 concentrations to be increased 3- and 2-fold, respectively between 20 diu and B. Maximal expression of rIGFBP-1 was at +1 dpn (220% B), and of rIGFBP-2, at B. Both rIGFBPs then decreased, reaching about 5% B at adulthood. All these data indicate that increased transcriptional activity of the rIGFBP-1 and rIGFBP-2 genes at birth would determine the increased synthesis in the liver and circulating levels of these proteins. In addition, it would seem that post-transcriptional events (reduced half-life of the rIGFBP-1 messenger after birth, translation efficiency of the rIGFBP-2 messenger) modulate transcriptional regulation.
Terabit/Sec VCSEL-Based 48-Channel Optical Module Based on Holey CMOS Transceiver IC (2012)

We report here on the design, fabrication and characterization of 48-channel parallel optical transceivers demonstrating terabit/sec data transfer rate. The 0.48 Tb/s transmit plus 0.48 Tb/s receive throughput was achieved using a second-generation single-chip holey CMOS transceiver IC. In addition to 24 receiver (RX) and 24 laser diode driver circuits, the 5.2 mm × 5.8 mm single CMOS chip incorporates 48 through-substrate optical vias (holes), one for each transmitter (TX) and RX channel. A complete holey Optochip is formed following direct flip-chip attachment of 24-channel 850-nm VCSEL and PD arrays. The 48 optical vias enable optical access to the 24 VCSELs and 24 PDs. The holey Optochip concept provides a dense chip-scale package which is fully compatible with industry-standard top emitting/detecting 850-nm VCSELs/PDs providing optimized high-speed performance through close integration of the optoelectronic (OE) devices with their drive electronics. Furthermore, the optical vias and OE devices are arranged in a 4 × 12 array on 250 μm × 250 μ m pitch to facilitate direct fiber-coupling to a standard 4 × 12 multi-mode fiber array. The Optochips are packaged into complete modules by flip-chip soldering to high-density, high-speed organic carriers. A pluggable connector soldered to the bottom of the carrier provides all module electrical I/O. The 18 mm × 18 mm overall module area is dictated by the 0.8 mm-pitch ball grid array (BGA) of the organic carrier and connector. Fully functional holey Optomodules with 24 TX and 24 RX channels operate up to 20 Gb/s/ch achieving efficiencies (including both TX and RX) of 7.3 pJ/bit. The terabit/sec data rate (480 Gb/s TX + 480 Gb/s RX) is highest reported for single-chip CMOS transceiver modules.
The research of camera distortion correction basing on neural network (2011)

Camera distortion correction is an important part of the camera calibration. Because camera distortion model is too difficult to be established, the BP neural network is used in camera distortion correction. The Hybrid algorithm which is the BP algorithm with PSO algorithm and chaos is introduced to the camera distortion correction. Weights and thresholds is optimized by the hybrid algorithm, and this hybrid algorithm can jump out of local optimum. So camera distortion is corrected by the hybrid algorithm.
Conjugated Polymers with Main Chain Chirality. 3. Synthesis of Optically Active Poly(aryleneethynylene)s (1996)

Binaphthyl-based chiral poly(aryleneethynylene)s of different molecular weights have been synthesized. An optically active poly(aryleneethynylene), (R)-9c, is obtained by coupling an optically active binaphthyl molecule, (R)-2,2‘-bis(octadecyloxy)-6,6‘-diethynyl-1,1‘-binaphthyl, (R)-8, with 1,4-diiodobenzene in the presence of tetrakis(triphenylphosphine)palladium(0) and cuprous iodide catalysts. GPC analysis of (R)-9c shows Mw = 29 000 and Mn = 12 000 (PDI = 2.4). Its specific optical rotation is [α]D = −272.2° (c = 0.5, CH2Cl2). Coupling of (R)-8 with 4,4‘-diiodobiphenyl using tetrakis(triphenylphosphine)palladium(0) and cuprous iodide catalysts also generates an optically active poly(aryleneethynylene), (R)-10c. GPC analysis of (R)-10c shows Mw = 37 000 and Mn = 18 000 (PDI = 2.1). The specific optical rotation of (R)-10c is [α]D = −281.7° (c = 0.18, CH2Cl2). A laser light scattering study of these polymers shows that the actual molecular weights (Mw) of these materials are higher (1.4−2.5 times) than those obtained from GPC analysis vs polystyrene standards. The repeat units of these polymers have been synthesized. UV spectroscopic studies of the repeat units and of the polymers demonstrate that the conjugation of the polymers is determined by the conjugation in their repeat units.
Circular DNA molecules imaged in air by scanning force microscopy (1992)

Routine and reproducible imaging of DNA molecules in air with the scanning force microscope (SFM) has been accomplished. Circular molecules of plasmid DNA were deposited onto red mica and imaged under various relative humidities. In related experiments, the first images of the Escherichia coli RNA polymerase-DNA complex have also been obtained. This has been possible by (1) the use of specially modified SFM tips with a consistent radius of curvature of 10 nm or less, to minimize the amount of image distortion introduced by the finite dimensions of commercially available tips, (2) the optimization of a method to deposit and bind DNA molecules to the mica surface in a stable fashion, and (3) careful control of the sample humidity, to prevent solvation of the molecules and detachment from the surface by the scanning tip or stylus. Contact forces in the range of a few nanonewtons are routinely possible in air and in the presence of residual humidity. The spatial resolution of the images appears determined by the radius of curvature of the modified styli, which can be estimated directly from the apparent widths of the DNA molecules in the images.
Central Executive and Default Mode Network Intra-network Functional Connectivity Patterns in Chronic Migraine (2018)

There are similar patterns of NTNC dysfunction within CEN in CM regardless of MOH status. We observed more extensive intranetwork disruption in CM<sup>MOH</sup> than CM. The decreased coherence between the right inferior frontal gyrus and the left dorsolateral PFC in CM<sup>MOH</sup> is likely associated with a significant disruption in the inhibitory control and a maladaptive response in risk aversion and reward; whereas the decreased coherence between right dorsolateral and ventrolateral PFC to contralateral dorsal PFC/FEF may be related to lack of cognitive control and top-down regulation of pain in both CM and CM<sup>MOH</sup>.
Composite hollow fiber membranes (1979)

Abstract Composite polysulfone hollow fibers consisting of a polysulfone porous substrate coated with crosslinked polyethyleneimine (PEI) or furan resin are reported. These composite hollow fibers are analogous to the flat‐sheet composite membranes known as NS‐100 and NS‐200. The surface structure of the porous substrate was rigorously studied before and after coating. Scanning electron microscope observations and reverse osmosis transport studies showed that the support fiber must have surface pore diameters of less than 0.2 μm to obtain a durable composite hollow fiber membrane. The curing process would normally follow in situ condensation of the PEI or the cationic polymerization of the furfuryl alcohol. However, since both the dense layer and surface of the porous substrate contract when exposed to the curing temperature (110–150°C), it was found to be profitable to cure the hollow fiber before applying the coating. When tested in a reverse osmosis rig, PEI/TDI‐coated polysulfone hollow fiber bundles displayed 98% salt rejection and a flux of 5–7 gfd for a feed solution of 10,000 ppm NaCl at a hydraulic pressure of 400 psi. A new method of depositing furan resin on the polysulfone hollow fiber is described. The furfuryl alcohol is instantaneously polymerized by exposing the alcohol‐soaked fiber to a 60% solution of concentrated sulfuric acid. It has been demonstrated that in such a polymerization procedure a dense, semipermeable layer is formed on top of the porous substrate; the resulting composite hollow fiber membrane yields salt rejections higher than 98% when tested under the above reverse osmosis conditions.
Photoreactive labeling of M13 coat protein in model membranes by use of a glycolipid probe. (1979)

Coliphage M13 coat protein in synthetic bilayer membranes was labeled by use of 12-(4-azide-2-nitrophenoxy)stearoyl[1-14C]glucosamine, a photoreactive glycolipid probe that spontaneously inserts into membranes. In this model system, the probe preferentially labeled the proteins over the lipids. Experiments designed to test the probe's restriction to integral membrane proteins revealed that extrinsic proteins as well as external peptide fragments of integral membrane proteins were not accessible to the photogenerated nitrene on the fatty acid chain. Only integral membrane peptides were labeled by the membrane-bound probe. These results indicate that protein labeling can be effected specifically from within the hydrocarbon milieu of a model membrane system.
A Review of Recent Advances in Plasma Polymerization (1979)

Polymers can be obtained by subjecting an organic or organometallic vapor to the plasma created in an electrical glow discharge. By varying the plasma conditions such as pressure, monomer flow rate, power etc., not only films but also powder and oil are formed In this paper we shall consider both the mechanism of polymerization and the influence of reaction conditions upon the rates of polymerization. A kinetic model will be presented, which appears to be in good agreement with experiments. The plasma polymerized materials can be characterized by IR, NMR, ESCA and other analytical techniques. It has been found that the polymer is highly crosslinked, amorphous and often contains functional groups not originally present in the monomer. The electrical properties of these films will also be discussed. Some of the potential applications of plasma polymerized films will be pointed out.
Toll-Like Receptor (TLR)-7 and -8 Modulatory Activities of Dimeric Imidazoquinolines (2012)

Toll-like receptors (TLRs) are pattern recognition receptors that recognize specific molecular patterns present in molecules that are broadly shared by pathogens but are structurally distinct from host molecules. The TLR7-agonistic imidazoquinolines are of interest as vaccine adjuvants given their ability to induce pronounced Th1-skewed humoral responses. Minor modifications on the imidazoquinoline scaffold result in TLR7-antagonistic compounds which may be of value in addressing innate immune activation-driven immune exhaustion observed in HIV. We describe the syntheses and evaluation of TLR7 and TLR8 modulatory activities of dimeric constructs of imidazoquinoline linked at the C2, C4, C8, and N(1)-aryl positions. Dimers linked at the C4, C8, and N(1)-aryl positions were agonistic at TLR7; only the N(1)-aryl dimer with a 12-carbon linker was dual TLR7/8 agonistic. Dimers linked at C2 position showed antagonistic activities at TLR7 and TLR8; the C2 dimer with a propylene spacer was maximally antagonistic at both TLR7 and TLR8.
Skin reactivity of unsensitized monkeys upon challenge with staphylococcal enterotoxin B: a new approach for investigating the site of toxin action (1985)

The correlation between skin tests and emetic responses in unsensitized monkeys was used to elucidate the cellular site of action of staphylococcal enterotoxin B (SEB). Evidence is presented that SEB administered intradermally provoked immediate-type skin reactions associated with mild degranulation of cutaneous mast cells. The cytoplasma showed signs of synthetic and metabolic activity, with formation of vesicles and increased prominence of mitochondria. Carboxymethylation of histidine residues of SEB altered the molecule (cSEB) from more alkaline components to more acidic species with increased microheterogeneity. This modification caused a loss in toxicity and completely abrogated the skin-sensitizing activity without changing the immunological specificity. cSEB, however, could compete with SEB for binding sites on the target cell surface. Previously, compound 48/80-treated skin sites behaved refractively to challenge with SEB, indicating that mediators from cutaneous mast cells are required for SEB-induced skin reactions. Skin reactions as well as emetic responses challenged with SEB were completely inhibited by H2 receptor antagonists and calcium channel blockers but not by H1 antihistamine or competitive antagonists of serotonin. This new approach provides a model for investigating the mechanisms of SEB action.
A Critical Period for Macromolecular Synthesis in Long-Term Heterosynaptic Facilitation in Aplysia (1986)

Both long-term and short-term sensitization of the gill and siphon withdrawal reflex in Aplysia involve facilitation of the monosynaptic connections between the sensory and motor neurons. To analyze the relationship between these two forms of synaptic facilitation at the cellular and molecular level, this monosynaptic sensorimotor component of the gill-withdrawal reflex of Aplysia can be reconstituted in dissociated cell culture. Whereas one brief application of 1 microM serotonin produced short-term facilitation in the sensorimotor connection that lasted minutes, five applications over 1.5 hours resulted in long-term facilitation that lasted more than 24 hours. Inhibitors of protein synthesis or RNA synthesis selectively blocked long-term facilitation, but not short-term facilitation, indicating that long-term facilitation requires the expression of gene products not essential for short-term facilitation. Moreover, the inhibitors only blocked long-term facilitation when given during the serotonin applications; the inhibitors did not block the facilitation when given either before or after serotonin application. These results parallel those for behavioral performance in vertebrates and indicate that the critical time window characteristic of the requirement for macromolecular synthesis in long-term heterosynaptic facilitation is not a property of complex circuitry, but an intrinsic characteristic of specific nerve cells and synaptic connections involved in the long-term storage of information.
Effect of CYP2C Polymorphisms on the Pharmacokinetics of Phenytoin in Japanese Patients with Epilepsy. (1996)

We examined the effect of CYP2C9/19 polymorphisms on the pharmacokinetics of phenytoin in 17 Japanese patients with epilepsy. The maximal elimination rate (Vmax) of phenytoin was slightly decreased (up to 14%) in patients with CYP2C19 mutations for the defective allele. The Vmax values in patients with a CYP2C9 mutation for the heterozygous Ile/Leu359 allele were 40% lower than those in patients with wild-type CYP2C9 for the homozygous Ile359 allele. These findings suggested that the genetic polymorphism of CYP2C isoenzymes plays an important role in the pharmacokinetic variability of phenytoin, and that the mutation in CYP2C9 proteins is a determinant of impaired metabolism of the drug.
Broad-phase collision detection using semi-adjusting BSP-trees (2005)

The broad-phase step of collision detection in scenes composed of n moving objects is a challenging problem because enumerating collision pairs has an inherent O(n2) complexity. Spatial data structures are designed to accelerate this process, but often their static nature makes it difficult to handle dynamic scenes. In this work we propose a new structure called Semi-Adjusting BSP-tree for representing scenes composed of thousands of moving objects. An scheduling algorithm evaluates locations where the BSP-tree becomes unbalanced, uses several strategies to alter cutting planes, and defer updates based on their re-structuring cost. We show that the tree does not require a complete re-structuring even in highly dynamic scenes, but adjusts itself while maintaining desirable balancing and height properties.
Prevention of Type 1 Diabetes with Major Histocompatibility Complex–Compatible and Nonmarrow Ablative Hematopoietic Stem Cell Transplants (2005)

Progression to hyperglycemia in young nonobese diabetic (NOD) mice is blocked by the transplantation of hematopoietic cells mismatched at the major histocompatibility complex (MHC). Because the NOD MHC class II allele, I-A(g7), is the primary disease susceptibility gene, it is logical to conclude that MHC-mismatched hematopoietic grafts prevent diabetes by replacement of this susceptibility allele on critical hematolymphoid populations. In this report, transplantation of MHC-matched purified hematopoietic stem cells (HSCs) pre-vented diabetes development in NOD mice, demonstrating that alleles of non-MHC background genes expressed on hematopoietic cells are sufficient to disrupt the autoaggressive process. Nonmarrow ablative conditioning was 100% protective, further showing that elimination of NOD hematopoiesis, including T-cells, was not required for the graft to block diabetes pathogenesis. The current standard clinical practice of hematopoietic cell transplantation uses donor/recipient pairs that are matched at the MHC. In our view, the principles established here using an MHC-matched engineered hematopoietic graft in conjunction with nonmarrow ablative conditioning to successfully block autoimmune diabetes sufficiently reduces the morbidity of the allogeneic transplantation procedure such that a similar approach can be translated to the treatment of human autoimmune disorders.
Identification of inhibitors of nitric oxide synthase that do not interact with the endothelial celll‐arginine transporter (1992)

The effects of inhibitors of nitric oxide (NO) synthase and other cationic amino acids on unidirectional L-arginine transport were studied in porcine aortic endothelial cells cultured in microwell plates or perfused in microcarrier columns. L-Homoarginine, L-lysine and L-ornithine inhibited transport of L-arginine. The NO synthase inhibitors NG-monomethyl-L-arginine and NG-iminoethyl-L-ornithine also reduced L-arginine uptake, whereas NG-nitro-L-arginine and its methyl-ester had no inhibitory effect. The ability to modulate selectively endothelial cell L-arginine transport or NO synthase activity will allow further characterization of the arginine transporter and its role in regulating NO biosynthesis.