gebhart/patrepeval huggingface dataset:
Development of an Enzyme-Linked Immunosorbent Assay for Quantitative Determination of Quizalofop-p-ethyl (2006)

Accurate quantification of quizalofop-p-ethyl is essential for it may do harm to humans and animals through both water and food. Currently, detection of quizalofop-p-ethyl mainly relies on methods such as gas chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry. Although these techniques are reliable, they are relatively expensive and time-consuming because of multistep sample cleanup. To address this, we developed a competitive indirect enzyme-linked immunosorbent assay (ciELISA) with a polyclonal antibody against quizalofop-p-ethyl that was generated in our lab. The IC(50) of detection was 0.03495 microg/mL, and the lowest detection limit reached 0.00192 microg/mL. Furthermore, the method had high specificity for it did not cross-react with other structure-related compounds. When water and soil samples that were fortified with quizalofop-p-ethyl were analyzed by this ELISA, recoveries were in the range of 89-110% from water and 81-108% from soil. Good correlations between this immunoassay and gas chromatography data were obtained for residues of quizalofop-p-ethyl in water and soil. Our data indicate that this method is a convenient analytical technique for monitoring quizalofop-p-ethyl in waters without extraction and the extra cleanup step and in soil without the cleanup step.
3-Hydroxy-3-methylglutaryl-coenzyme A reductase from Haloferax volcanii: purification, characterization, and expression in Escherichia coli (1996)

Prior work from this laboratory characterized eukaryotic (hamster) and eubacterial (Pseudomonas mevalonii) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductases. We report here the characterization of an HMG-CoA reductase from the third domain, the archaea. HMG-CoA reductase of the halobacterium Haloferax volcanii was initially partially purified from extracts of H. volcanii. Subsequently, a portion of the H. volcanii lovastatin (formerly called mevinolin) resistance marker mev was subcloned into the Escherichia coli expression vector pT7-7. While no HMG-CoA reductase activity was detectable following expression in E. coli, activity could be recovered after extracts were exposed to 3 M KCl. Following purification to electrophoretic homogeneity, the specific activity of the expressed enzyme, 24 microU/mg, equaled that of homogeneous hamster or P. mevalonii HMG-CoA reductase. Activity was optimal at pH 7.3. Kms were 66 microM (NADPH) and 60 microM [(S)-HMG-CoA]. (R)-HMG-CoA and lovastatin inhibited competitively with (S)-HMG-CoA. H. volcanii HMG-CoA reductase also catalyzed the reduction of mevaldehyde [optimal activity at pH 6.0; Vmax 11 microU/mg; Kms 32 microM (NADPH), 550 microM [(R,S)-mevaldehyde]] and the oxidative acylation of mevaldehyde [optimal activity at pH 8.0; Vmax 2.1 microU/mg; Kms 350 microM (NADP+), 300 microM (CoA), 470 microM [(R,S)-mevaldehyde]]. These properties are comparable to those of hamster and P. mevalonii HMG-CoA reductases, suggesting a similar catalytic mechanism.
Lignin-based polyurethane materials (2008)

Four technical lignins (Alcell, Indulin AT, Sarkanda and Curan 27-11P) were used as macromonomers in the synthesis of polyurethane materials following two global approaches. In the first one Alcell and Indulin AT lignins were used directly as co-monomers in combination with a linear polycaprolactone (PCL) in order to produce polyurethane elastomers where lignin content varied between 10 and 25% (w/w) with respect to polyol mixture (PCL+lignin). The thermomechanical properties of the resulting materials were determined by dynamical mechanical analysis (DMA), differential scanning calorimetry (DSC) and swelling tests. In lignin-based elastomers Indulin AT showed to be more efficiently incorporated in the polyurethane network compared with Alcell lignin. Elastomers prepared with Indulin AT lignin exhibited a cross-linking density and storage modulus (rubbery plateau) higher than those of Alcell lignin-based counterpart and a lower soluble fraction. For both Alcell and Indulin AT based elastomers the glass transition temperature increased and extended over a wide temperature range with the increase of lignin content. The second approach consisted of producing rigid polyurethane foams (RPU) using ligninbased polyols obtained after chemical modification by an oxypropylation procedure. Two polyol formulations (20/80 and 30/70, in what concerns the weight ratios between lignin and propylene oxide, PO), were used in RPU formulations and their content varied from 0 to 100% (w/w with respect to a commercial polyol, used as a reference). The resulting RPU foams were characterized in terms of density, mechanical properties, conductivity and morphology. The prepared RPU foams with lignin-based polyols presented properties, very similar to those obtained from conventional commercial polyols. RPU foams prepared with 30/70 polyols exhibited improved properties comparatively to those arising from 20/80 formulations. Exceptions were however detected in RPU foams prepared with all Sarkanda lignin based polyols and Curan 27-11P 30/70 formulation, which were found to be inadequate for RPU formulation.
A 31%-efficient GaAs/silicon mechanically stacked, multijunction concentrator solar cell (1988)

The development and demonstration of the first solar cell to achieve an efficiency in excess of 30% are reported. The improved performance compared to previous GaAs/silicon mechanically stacked, multijunction (MSMJ) concentrator cells is due to improvements in the component cell technologies and to better optimization of the GaAs cell transmissivity. Preliminary analysis suggests that an efficiency approaching 35% is possible with GaAs-based MSMJ cells.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Tracking football players with multiple cameras (2005)

A system is described for tracking the positions of football players during a match. The input is eight video streams from static cameras, each processed to generate measurements of the players for a multi view tracker. The single view processing includes foreground detection and a bounding box tracker designed to split measurements of merged players. In the multi-view process, the measurements, each from a different camera and matched to the same player, are fused into an overall measurement which is then used in the estimate updating. Results are demonstrated on real data.
Synthesis and UV Photolysis of Oligodeoxynucleotides That Contain 5-(Phenylthiomethyl)-2‘-deoxyuridine:  A Specific Photolabile Precursor of 5-(2‘-Deoxyuridilyl)methyl Radical (2000)

[formula: see text] The title exocyclic radical (2) is generated via photochemical cleavage of 5-(phenylthiomethyl)-2'-deoxyuridine (8). The latter thionucleoside (8) was successfully incorporated into DNA oligomers by automated DNA synthesis using phosphoramidite chemistry. UV exposure of 8 containing oligonucleotides under (an)aerobic conditions gives rise to specific base lesions. The photoproducts have been isolated and further characterized on the basis of detailed NMR and mass spectrometric analyses.
Synthesis and antitumor and antiviral properties of 5-alkyl-2'-deoxyuridines 3',5'-cyclic monophosphates, and neutral cyclic triesters (1986)

A series of 5-alkyl-2'-deoxyuridine 3',5'-cyclic monophosphates (5-R-cdUMP's, R = Et, i-Pr, n-Pr, n-Bu, n-Pent, n-Hex, n-Oct) was prepared and tested in culture systems as antitumor and antiviral agents in comparison to the 5-alkyl-2'-deoxyuridines (5-R-dUrd's) themselves. Only the 5-Et- and 5-n-Bu-cdUMP showed appreciable cytostatic activities against murine L1210 and human lymphoblast Raji cells (ID50 range: 28-82 micrograms/mL). 5-Et-dUrd itself was much more active (ID50 = 1.6 and 2.9 micrograms/mL). The 5-i-Pr-, and 5-n-Bu-dUrd's were inactive, but activity increased again for groups with chain lengths of five carbons or greater. 5-Et-cdUMP and 5-Et-dUrd had greatly reduced activities against deoxythymidine kinase deficient (TK-) L1210 and Raji cells. 5-Et-cdUMP evidently is not an efficient prodrug source of the corresponding 5'-monophosphate where the TK- cells are concerned. Of the 5-R-cdUMP's, 5-Et-cdUMP displayed reasonably good antiviral potency against herpes simplex types 1 and 2 (MIC50, mostly 7-70 micrograms/mL) and vaccinia virus (MIC, 70 micrograms/mL). The activity was nonetheless 10- to 100-fold less than that for 5-Et-dUrd. The other 5-R-dUrd's generally showed decreasing antiviral activity with increasing 5-R chain length. Methyl and/or benzyl neutral triesters of certain 5-R-cdUMP's were inactive as antivirals and largely inactive against tumor cells in culture. In contrast to the 5'-monophosphates, the 5-R-cdUMP's failed to inhibit thymidylate synthetase from L1210 cells.
Globally Normalized Transition-Based Neural Networks (2016)

Daniel Andor, Chris Alberti, David Weiss, Aliaksei Severyn, Alessandro Presta, Kuzman Ganchev, Slav Petrov, Michael Collins. Proceedings of the 54th Annual Meeting of the Association for Computational Linguistics (Volume 1: Long Papers). 2016.
On the allocation of documents in multiprocessor information retrieval systems (1991)

Article On the allocation of documents in multiprocessor information retrieval systems Share on Authors: Ophir Frieder Dept. of Computer Science, George Mason University, Fairfax, VA Dept. of Computer Science, George Mason University, Fairfax, VAView Profile , Hava Tova Siegelmann Dept. of Computer Science, New Brunswick, NJ Dept. of Computer Science, New Brunswick, NJView Profile Authors Info & Claims SIGIR '91: Proceedings of the 14th annual international ACM SIGIR conference on Research and development in information retrievalSeptember 1991 Pages 230–239https://doi.org/10.1145/122860.122884Online:01 September 1991Publication History 21citation303DownloadsMetricsTotal Citations21Total Downloads303Last 12 Months2Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
352 Cryopreserved Gal-Knockout Xenografts Provide Efficacious Temporary Coverage of Full-Thickness Wounds: Good Laboratory Practice-Compliant Studies in Non-Human Primates (2019)

Split-thickness skin grafts derived from genetically engineered, Designated Pathogen Free (DPF), alpha 1,3 galactosyltransferase knockout miniature swine were tested alongside allograft comparators, over a two-phase Good Laboratory Practice (GLP)-compliant study series in non-human primates (NHP) for assessment of temporary coverage of full-thickness wounds. Xenografts were surgically harvested under aseptic conditions via dermatome, sterilely processed, and cryopreserved via control-rate freezer. Full-thickness wounds (4-9 cm2) were created on the dorsal region of 8 cynomolgus monkeys and grafted with xenografts and/or allografts (from a common NHP donor). All subjects were assessed for signs of graft adherence to the wound bed, vascularity, and visible signs of graft rejection. In phase 1, 4 male subjects each received 2 xeno (n=8) and 2 allo (n=8) grafts. On Days 12 and 15, all grafts were removed per study design for analysis; 8 wounds were then treated with split-thickness autografts. In phase 2, 4 mixed gender subjects were treated with 2 xenografts (only) (n=8). Study duration was extended and recipient-immune response was assessed. In phase 1, all xeno-and-allografts (n=16) were completely adherent to the surrounding wound bed and exhibited equivalent degrees of minimal epidermolysis at Days 12 and 15. Subjects treated with xenografts exhibited post-autograft healing equivalent to the allografts comparators. In phase 2, all xenografts (n=8) demonstrated adherence until the end-of-study at Day 30. Classic signs of graft rejection were absent at anticipated time points (10-14 days expected, based on previous experience). All subjects completed Phase 2 without adverse event. These preclinical investigations exceeded previously reported efficacy findings of similar, specialized xenografts. The xenografts performed indistinguishably from in-situ allograft comparators. These observations suggest that source material from DPF animals, harvested under controlled conditions, can significantly extend the period of graft adherence, a promising advance for patients with severe burns. These studies support an Investigational New Drug application; a Phase I clinical trial of GalT-KO Xeno-Skin™ grafts is planned for early 2019, pending final FDA approval.
Emerging Markets for China's Tourism Industry (1992)

International tourist arrivals showed a rebound in 1990 after the devastating loss in 1989 due to political turmoil. An analysis of the top five tourist-generating countries or regions reveals Taiwan and the U.S.S.R. as the two emerging markets for China's tourism industry. The author discusses the forces shaping such new international travel patterns and suggests further research needs on the new markets.
Production of angkak through co-culture of Monascus purpureus and Monascus ruber (2010)

Angkak (red mold rice, red yeast rice, Chinese red rice) is a traditional Chinese medicine produced by solid-state fermentation of cooked non-glutinous rice with Monascus species. The secondary metabolite of Monascus species, monacolin K /lovastatin, has been proven to lower blood lipid levels. In this study, a co-culture of Monascus purpureus MTCC 369 and Monascus ruber MTCC 1880 was used for angkak production. Four medium parameters screened by Plackett-Burman design were optimized by response surface methodology for highest lovastatin production in angkak during solid-state fermentation by the co-culture. Maximum lovastatin production of 2.84 mg g(-1) was predicted in solid medium containing 20 g rice and 40 ml liquid nutrients medium (malt extract 9.68 g l(-1), dextrose 38.90 g l(-1), MnSO4.H2O 1.96 g l(-1), and MgSO4.7H2O 0.730 g l(-1)) by point prediction tool of Design Expert 7.1 software (Statease Inc. USA).
Prodrugs of 9-Benzyl-8-hydroxy-2-(2-hydroxyethylthio)adenine: Potent Interferon Inducing Agents in Monkeys (2004)

In order to improve the oral bioavailability of 9-benzyl-8-hydroxy-2-(2-hydroxyethylthio)adenine (SM-295072), a potent interferon (IFN) inducing agent, we synthesized prodrugs of it by utilizing the hydroxy groups at the C(2)-side chain and/or the C(8)-position. The carbonate prodrug at the C(8)-position was more effective than that at the C(2)-side chain for oral absorption in rats. Among the compounds prepared, compound 6 demonstrated the most preferable prodrug properties, and the maximum plasma concentration of 6 was approximately 4-fold higher than that of SM-295072. Furthermore, compound 6 was dose-dependently absorbed in monkeys by oral administration, and exhibited a potent IFN-inducting activity that correlated well with its plasma drug concentration.
Transduction of murine cerebellar neurons with recombinant FIV and AAV5 vectors (2000)

Our data demonstrate that vectors derived from recombinant feline immunodeficiency virus (rFIV) and adeno-associated virus type 5 (rAAV5) transduce cerebellar cells following direct injection into the cerebellar lobules of mice. Both recombinant viruses mediated gene transfer predominantly to neurons, with up to 2500 and 1500 Purkinje cells transduced for rAAV5 or rFIV-based vectors, respectively. The vectors also transduced stellate, basket and Golgi neurons, with occasional transduction of granule cells and deep cerebellar nuclei. rAAV5 also spread outside the cerebellum to the inferior colliculus and ventricular epithelium, while rFIV demonstrated the ability to undergo retrograde transport to the physically close lateral vestibular nuclei. Thus, AAV5 and FIV-based vectors show promise for targeting neurons affected in the hereditary spinocerebellar ataxias. These vectors could be important tools for unraveling the pathophysiology of these disorders, or in testing factors which may promote neuronal survival.
Concise and Efficient Synthesis of 4-Fluoro-1H-pyrrolo[2,3-b]pyridine (2003)

Two routes describing the preparation of 4-fluoro-1H-pyrrolo[2,3-b]pyridine (4a) from 1H-pyrrolo[2,3-b]pyridine N-oxide (1) are presented. Regioselective fluorination was achieved using either the Balz-Schiemann reaction or lithium-halogen exchange. [reaction: see text]
Observing Majorana bound States inp-Wave Superconductors Using Noise Measurements in Tunneling Experiments (2007)

The zero-energy bound states at the edges or vortex cores of chiral p-wave superconductors should behave like Majorana fermions. We introduce a model Hamiltonian that describes the tunneling process when electrons are injected into such states. Using a nonequilibrium Green function formalism, we find exact analytic expressions for the tunneling current and noise and identify experimental signatures of the Majorana nature of the bound states to be found in the shot noise. We discuss the results in the context of different candidate materials that support triplet superconductivity. Experimental verification of the Majorana character of midgap states would have important implications for the prospects of topological quantum computation.
Structural diversity of the major surface antigen of Plasmodium falciparum merozoites. (1986)

The structures of the major merozoite surface antigen of Plasmodium falciparum and the gene encoding it were indistinguishable for the Wellcome strain and the Thai clone T9/94 but different for clones T9/96, T9/98, and T9/101. The central portion of the gene is subject to the greatest variation in structure. The protein from all five lines was found to be posttranslationally modified by covalent addition of both carbohydrate and fatty acid.
Drug Discovery for Schistosomiasis: Hit and Lead Compounds Identified in a Library of Known Drugs by Medium-Throughput Phenotypic Screening (2009)

To accelerate the identification of novel anti-schistosomals, we have developed a partially automated screen workflow that interfaces schistosomula with microtiter plate-formatted compound libraries. The workflow has identified various compounds and drugs as hits in vitro and leads, with the prescribed oral efficacy, in vivo. Efforts to improve throughput, automation, and rigor of the screening workflow are ongoing.
Nickel(0)-Catalyzed Cycloaddition Copolymerization of Ether Diynes with Carbon Dioxide to Poly(2-pyrone)s (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNickel(0)-Catalyzed Cycloaddition Copolymerization of Ether Diynes with Carbon Dioxide to Poly(2-pyrone)sTetsuo Tsuda, Hiroyuki Yasukawa, and Kenji KomoriCite this: Macromolecules 1995, 28, 5, 1356–1359Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://doi.org/10.1021/ma00109a004RIGHTS & PERMISSIONSArticle Views153Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (551 KB) Get e-Alerts Get e-Alerts
Clinical Pharmacology of Vasodilator Peptides (1987)

Summary: Several neuropeptides cause mild flushing when infused intravenously in humans. However, only calcitonin gene-related peptide (CGRP), vasointestinal peptide, and substance P (SP) cause reproducible falls in blood pressure when infused into normal subjects. Of these, CGRP is of the most interest because it is the most potent and because it is present in vascular nerve endings. This paper summarises the evidence to date suggesting that CGRP release from vascular nerve endings may be of physiological importance in the regulation of blood pressure.
Transcription of Bacillus subtilis subtilisin and expression of subtilisin in sporulation mutants (1988)

The start point for transcription of the subtilisin (aprE) gene was determined by primer extension analysis and was found to be at a point significantly different from that identified in a previously published report (S. L. Wong, C. W. Price, D. S. Goldfarb, and R. H. Doi, Proc. Natl. Acad. Sci. USA 81:1184-1188, 1984). An aprE-lacZ fusion was used to analyze expression of the promoter. Deletion analyses of the promoter were performed to determine the extent of the upstream region necessary for activity. This was found to be between -52 and -41 with respect to the transcription start site. Expression of the aprE-lacZ fusion was unimpaired in a mutant deleted for the sigma B subunit of RNA polymerase. Mutations in the gene for the sigma H subunit of RNA polymerase decreased expression of the aprE-lacZ fusion to approximately 25% of that of the wild type. These results leave the identity of the sigma factor responsible for transcription of this gene in question. Mutations in the spo0A gene drastically decreased the activity of the aprE promoter and its upstream deletion derivatives, while the abrB gene, a phenotypic suppressor of spo0 mutations, restored activity of the aprE promoter in all of the deletion derivatives. Thus, inhibition of transcription by the spo0A mutation and its restoration by an abrB mutation could not be separated from the promoter of the aprE gene.
Seismic waves in a stratified half space (1979)

The response of a stratified elastic half space to a general source may be represented in terms of the reflection and transmission properties of the regions above and below the source. For P-SV and SH waves and both buried sources and receivers, convenient forms of the response may be found in which no loss of precision problems arise from growing exponential terms in the evanescent regime. These expressions have a ready physical interpretation and enable useful approximations to the response to be developed. The reflection representation leads to efficient computational procedures for models composed of uniform layers, which may be extended in an asymptotic development to piecewise smooth models.