gebhart/patrepeval huggingface dataset:
Renal interaction between sympathetic activity and ANP in rats with chronic ischaemic heart failure (1989)

The diuretic and natriuretic effects of r-alpha-ANP (99-126) were investigated in rats with chronic ischaemic heart failure (IHF) produced by left coronary artery ligation. The plasma concentration of immunoreactive ANP (IrANP) was significantly higher, 91.8 +/- 16.0 pm in the IHF rats compared to 31.0 +/- 4.9 pm in sham-operated controls. In the control rats, ANP infusion (0.25-1.0 micrograms kg 1 mm 1) increased urine flow rate (V) and urinary sodium (UNa V) excretion. At the highest dose level, both V and UNa V were increased approximately fivefold. The diuresis and natriuresis seen in the control group after the infusion of ANP were blunted in the IHF rats. A bilateral surgical renal denervation in the IHF rats did not alter the renal dopamine levels, but induced a significant decrease in renal noradrenaline content, and almost completely restored the renal responsiveness to the ANP infusions. We conclude that renal denervation reversed the blunted renal excretory response to ANP in IHF rats. Thus, in experimental IHF, there seems to be a functional antagonism between efferent renal sympathetic nerve activity and ANP.
Cost analysis of supercapacitor cell production (2013)

A life cycle costing (LCC) is to be performed complementary to the ongoing research on an enhanced supercapacitor pouch cell, in order to provide additional decision support on the best cell chemistry from the economic point of view. Due to the early stage of the project so far merely the production phase is considered. The detailed cost calculation method was chosen and complemented with a scale up using dimension analysis and analogy analysis, in order to be able to utilize this method since available data is either scarce or refers to laboratory scale. It was found that the researched cells are within the lower margin of costs reported in literature. Also the relative contribution of material and production costs as well as energy consumption was in the same range as stated in literature. Although these comparisons should be handled with care as they do not always refer to the exact same item. Further, we concluded that the developed approach provides a sound basis for a reproducible calculation of production costs for technologies at an early research stage.
Gene Transfer into Mammalian Cells Using Histone-Condensed Plasmid DNA (1996)

A recombinant histone (NLS-H1) containing both the SV40 large T antigen nuclear localization signal and the carboxy-terminal domain of human histone H1(0) was produced in bacteria. NLS-H1-plasmid DNA complexes, in the presence of chloroquine, mediated reporter gene transfer into cultured cells with similar efficiencies as plasmid DNA-cationic lipid (lipofectin) complexes. NIH-3T3 or COS-7 cells transfected with NLS-H1-plasmid DNA-lipofectin complexes expressed at least 20 times more luciferase or had at least 2.5 times more beta-galactosidase-positive cells than those transfected with plasmid DNA-lipofectin complexes. Foreign gene expression was also improved by other DNA-binding proteins and cationic lipid formulations, yet the greatest enhancement was obtained with complexes containing either NLS-H1 or calf thymus histone H1. Histone H1-plasmid DNA-lipofectin complexes were internalized by a greater number of cells than plasmid DNA-lipofectin complexes.
Golgi protein FAPP2 tubulates membranes (2009)

The Golgi-associated four-phosphate adaptor protein 2 (FAPP2) has been shown to possess transfer activity for glucosylceramide both in vitro and in cells. We have previously shown that FAPP2 is involved in apical transport from the Golgi complex in epithelial MDCK cells. In this paper we assign an unknown activity for the protein as well as providing structural insight into protein assembly and a low-resolution envelope structure. By applying analytical ultracentrifugation and small-angle x-ray scattering, we show that FAPP2 is a dimeric protein in solution, having a curved shape 30 nm in length. The purified FAPP2 protein has the capability to form tubules from membrane sheets in vitro. This activity is dependent on the phosphoinositide-binding activity of the PH domain of FAPP2. These data suggest that FAPP2 functions directly in the formation of apical carriers in the trans-Golgi network.
Stat5a is mandatory for adult mammary gland development and lactogenesis. (1997)

Prolactin (PRL) induces mammary gland development (defined as mammopoiesis) and lactogenesis. Binding of PRL to its receptor leads to the phosphorylation and activation of STAT (signal transducers and activators of transcription) proteins, which in turn promote the expression of specific genes. The activity pattern of two STAT proteins, Stat5a and Stat5b, in mammary tissue during pregnancy suggests an active role for these transcription factors in epithelial cell differentiation and milk protein gene expression. To investigate the function of Stat5a in mammopoiesis and lactogenesis we disrupted this gene in mice by gene targeting. Stat5a-deficient mice developed normally and were indistinguishable from hemizygous and wild-type littermates in size, weight, and fertility. However, mammary lobuloalveolar outgrowth during pregnancy was curtailed, and females failed to lactate after parturition because of a failure of terminal differentiation. Although Stat5b has a 96% similarity with Stat5a and a superimposable expression pattern during mammary gland development it failed to counterbalance for the absence of Stat5a. These results document that Stat5a is the principal and an obligate mediator of mammopoietic and lactogenic signaling.
IL-17 produced by neutrophils regulates IFN-γ–mediated neutrophil migration in mouse kidney ischemia-reperfusion injury (2009)

The IL-23/IL-17 and IL-12/IFN-gamma cytokine pathways have a role in chronic autoimmunity, which is considered mainly a dysfunction of adaptive immunity. The extent to which they contribute to innate immunity is, however, unknown. We used a mouse model of acute kidney ischemia-reperfusion injury (IRI) to test the hypothesis that early production of IL-23 and IL-12 following IRI activates downstream IL-17 and IFN-gamma signaling pathways and promotes kidney inflammation. Deficiency in IL-23, IL-17A, or IL-17 receptor (IL-17R) and mAb neutralization of CXCR2, the p19 subunit of IL-23, or IL-17A attenuated neutrophil infiltration in acute kidney IRI in mice. We further demonstrate that IL-17A produced by GR-1+ neutrophils was critical for kidney IRI in mice. Activation of the IL-12/IFN-gamma pathway and NKT cells by administering alpha-galactosylceramide-primed bone marrow-derived DCs increased IFN-gamma production following moderate IRI in WT mice but did not exacerbate injury or enhance IFN-gamma production in either Il17a-/- or Il17r-/- mice, which suggested that IL-17 signaling was proximal to IFN-gamma signaling. This was confirmed by the finding that IFN-gamma administration reversed the protection seen in Il17a-/- mice subjected to IRI, whereas IL-17A failed to reverse protection in Ifng-/- mice. These results demonstrate that the innate immune component of kidney IRI requires dual activation of the IL-12/IFN-gamma and IL-23/IL-17 signaling pathways and that neutrophil production of IL-17A is upstream of IL-12/IFN-gamma. These mechanisms might contribute to reperfusion injury in other organs.
Oxidative Etching and Its Role in Manipulating the Nucleation and Growth of Noble-Metal Nanocrystals (2013)

Oxidative etching plays an important role in the synthesis of metal nanocrystals. This is because the zerovalent species, including atoms, clusters, and nanocrystallites, can all be oxidized back to the ionic form and thus altering the types and distributions of products formed in both the nucleation and growth steps. In the first part of this review, we discuss the critical components needed for oxidative etching, as well as methods for enabling or preventing oxidative etching in a synthesis. We then present and analyze a number of interesting experimental observations caused by oxidative etching. In the following sections, we highlight four major applications of oxidative etching in the synthesis of metal nanocrystals, with regards to experimental controls over the crystallinity, size, shape, morphology, and growth kinetics.
Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum (1998)

Exposure of the ileum to nutrients markedly inhibits several upper gastrointestinal functions. Hormonal peptides of the ileal wall, i.e. peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NT), are thought to play a role in this negative feedback mechanism. The present study was conducted to comparatively assess the secretion of PYY, GLP-1, and NT upon luminal infusion of a variety of individual luminal factors in the isolated vascularly perfused rat ileum preparation. PYY, GLP-1, and NT were measured in the portal effluent with specific RIAs. Glucose (250 mm) induced a pronounced release of the three peptides, whereas a physiological concentration of 5 mm did not induce peptide secretion. Peptone (5%, wt/vol) evoked a sustained release of PYY, GLP-1, and NT. Only NT secretion was increased upon luminal administration of 100 mm sodium oleate. Short chain fatty acids (20 mm) evoked an early and transient release of the three peptides. In contrast, taurocholate (20 mm) induced a sustained release of PYY, GLP-1, and NT, but the threshold concentration for peptide release was lower for NT than for PYY or GLP-1. Cellulose or pectin (0.5%, wt/vol) did not modify peptide secretion. In conclusion, glucose and peptone are potent stimulants of PYY, GLP-1, and NT release. Only NT is released upon oleic acid stimulation. Finally, taurocholate is a potent stimulant of the release of the three peptides. Overall, PYY, GLP-1, and NT may participate cooperatively in the ileal brake. As relatively high concentrations of the various stimulants were required to elicit peptide release, it seems likely that this mechanism operates in cases of maldigestion or malabsorption.
M olecular D efects in H uman S evere C ombined I mmunodeficiency and A pproaches to I mmune R econstitution (2004)

Mutations in nine different genes have been found to cause the human severe combined immunodeficiency syndrome. The products of three of the genes--IL-2RG, Jak3, and IL-7R alpha--are components of cytokine receptors, and the products of three more-RAG1, RAG2, and Artemis-are essential for effecting antigen receptor gene rearrangement. Additionally, a deficiency of CD3 delta, a component of the T-cell antigen receptor, results in a near absence of circulating mature CD3+ T cells and a complete lack of gamma/delta T cells. Adenosine deaminase deficiency results in toxic accumulations of metabolites that cause T cell apoptosis. Finally, a deficiency of CD45, a critical regulator of signaling thresholds in immune cells, also causes SCID. Approaches to immune reconstitution have included bone marrow transplantation and gene therapy. Bone marrow transplantation, both HLA identical unfractionated and T cell-depleted HLA haploidentical, has been very successful in effecting immune reconstitution if done in the first 3.5 months of life and without pretransplant chemotherapy. Gene therapy was highly successful in nine infants with X-linked SCID, but the trials have been placed on hold due to the development of a leukemic process in two of the children because of insertional oncogenesis.
Brief Focal Cerebral Ischemia That Simulates Transient Ischemic Attacks in Humans Regulates Gene Expression in Rat Peripheral Blood (2009)

Blood gene expression profiles of very brief (5 and 10 mins) focal ischemia that simulates transient ischemic attacks in humans were compared with ischemic stroke (120 mins focal ischemia), sham, and naïve controls. The number of significantly regulated genes after 5 and 10 mins of cerebral ischemia was 39 and 160, respectively (fold change >/=mid R:1.5mid R: and P<0.05). There were 103 genes common to brief focal ischemia and ischemic stroke. Ingenuity pathway analysis showed that genes regulated in the 5 mins group were mainly involved in small molecule biochemistry. Genes regulated in the 10 mins group were involved in cell death, development, growth, and proliferation. Such genes were also regulated in the ischemic stroke group. Genes common to ischemia were involved in the inflammatory response, immune response, and cell death-indicating that these pathways are a feature of focal ischemia, regardless of the duration. These results provide evidence that brief focal ischemia differentially regulates gene expression in the peripheral blood in a manner that could distinguish brief focal ischemia from ischemic stroke and controls in rats. We postulate that this will also occur in humans.
Neurocognitive effects of brivaracetam, levetiracetam, and lorazepam (2010)

The differential pattern of drug effects was consistent across multiple electrophysiologic, cognitive, and subjective measures. The profile of cognitive, subjective, and electrophysiologic effects for BRV was similar to the analog compound LEV and to placebo. The findings suggest that BRV should be tolerated well from a neuropsychological perspective, but additional studies are needed.
Epidermal growth factor receptor inhibitors in non-small-cell lung cancer (2007)

The epidermal growth factor receptor (EGFR) is a cell membrane receptor that plays a key role in cancer development and in the progression of many human malignancies, including non-small-cell lung cancer (NSCLC). EGFR-dependent signaling is involved in cancer cell proliferation, apoptosis, angiogenesis, invasion and metastasis. Targeting the EGFR is a valuable molecular approach in cancer therapy. This receptor is overexpressed in up to 80% of NSCLC cases. Thus, several molecules inhibiting this critical biologic pathway have been synthesized and tested as a single agent or in combination with other anticancer modalities in a wide of clinical trials, including reversible and irreversible small tyrosine kinase inhibitors, such as gefitinib and erlotinib, dual vascular endothelial growth factor receptor EGFR tyrosine kinase inhibitors, such as vandetanib (ZD-6474), and monoclonal antibodies, such as cetuximab, which have shown promising activity in patients with NSCLC. This review focuses on the preclinical and clinical results available with EGFR inhibitors in the treatment of NSCLC patients.
Vaccinia and Pox-Virus (2007)

Vaccinia virus has been studied extensively since its discovery as a smallpox vaccine in 1798. Its use as a smallpox vaccine documented its safety profile. It was later found that its large size and ability to accept large fragments of DNA combined with its natural tumor affinity make it an attractive agent for cancer therapy. This chapter discusses the history of the vaccinia, the various strains available, the biology of the virus as well as the steps in creating recombinants. The various clinical and safety considerations will be addressed. We will also discuss the various methods used to treat cancer using the vaccinia virus and will review the recent clinical trials using vaccinia in the treatment of cancer.
Signal analysis through analog representation (2003)

We present an approach to the analysis of signals based on analog representation of measurements. Methodologically, it relies on the consideration and full use of the continuous nature of a realistic, as opposed to an idealized, measuring process. Mathematically, it is based on the transformation of discrete or continuous signals into normalized continuous scalar fields with the mathematical properties of distribution functions. This approach allows a simple and efficient implementation of many traditionally digital analysis tools, including nonlinear filtering techniques based on order statistics. It also enables the introduction of a large variety of new characteristics of both one– and multi–dimensional signals, which have no digital counterparts.
Enantioselective Addition of Organometallic Reagents to Carbonyl Compounds: Chirality Transfer, Multiplication, and Amplification (1991)

Abstract Nucleophilic addition of organometallic reagents to carbonyl substrates constitutes one of the most fundamental operations in organic synthesis. Modification of the organometallic compounds by chiral, nonracemic auxiliaries offers a general opportunity to create optically active alcohols, and the catalytic version in particular provides maximum synthetic efficiency. The use of organozinc chemistry, unlike conventional organolithium or ‐magnesium chemistry, has realized an ideal catalytic enantioselective alkylation of aldehydes leading to a diverse array of secondary alcohols of high optical purity. A combination of dialkylzinc compounds and certain sterically constrained β‐dialkylamino alcohols, such as (–)‐3‐ exo ‐dimethylaminoiso‐ borneol [(–)‐DAIB], as chiral inducers affords the best result (up to 99% ee ). The alkyl transfer reaction occurs via a dinuclear Zn complex containing a chiral amino alkoxide, an aldehyde ligand, and three alkyl groups. The chiral multiplication method exhibits enormous chiral amplification: a high level of enantioselection (up to 98%) is attainable by use of DAIB in 14% ee . This unusual nonlinear effect is a result of a marked difference in chemical properties of the diastereomeric (homochiral and heterochiral) dinuclear complexes formed from the dialkylzinc and the DAIB auxiliary. This phenomenon may be the beginning of a new generation of enantioselective organic reactions.
Persistent high frequency of human immunodeficiency virus-specific cytotoxic T cells in peripheral blood of infected donors. (1995)

Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTLs) are thought to play a major role in the immune response to HIV infection. The HIV-specific CTL response is much stronger than previously documented in an infectious disease, yet estimates of CTL frequency derived from limiting-dilution analysis (LDA) are relatively low and comparable to other viral infections. Here we show that individual CTL clones specific for peptides from HIV gag and pol gene products are present at high levels in the peripheral blood of three infected patients and that individual CTL clones may represent between 0.2% and 1% of T cells. Previous LDA in one donor had shown a frequency of CTL precursors of 1/8000, suggesting that LDA may underestimate CTL effector frequency. In some donors individual CTL clones persisted in vivo for at least 5 years. In contrast, in one patient there was a switch in CTL usage suggesting that different populations of CTLs can be recruited during infection. These data imply strong stimulation of CTLs, potentially leading some clones to exhaustion.
Efficiency Analysis of Waveform Shape for Electrical Excitation of Nerve Fibers (2010)

Stimulation efficiency is an important consideration in the stimulation parameters of implantable neural stimulators. The objective of this study was to analyze the effects of waveform shape and duration on the charge, power, and energy efficiency of neural stimulation. Using a population model of mammalian axons and in vivo experiments on cat sciatic nerve, we analyzed the stimulation efficiency of four waveform shapes: square, rising exponential, decaying exponential, and rising ramp. No waveform was simultaneously energy-, charge-, and power-optimal, and differences in efficiency among waveform shapes varied with pulse width (PW). For short PWs (< or = 0.1 ms), square waveforms were no less energy-efficient than exponential waveforms, and the most charge-efficient shape was the ramp. For long PW s (> or = 0.5 ms), the square was the least energy-efficient and charge-efficient shape, but across most PW s, the square was the most power-efficient shape. Rising exponentials provided no practical gains in efficiency over the other shapes, and our results refute previous claims that the rising exponential is the energy-optimal shape. An improved understanding of how stimulation parameters affect stimulation efficiency will help improve the design and programming of implantable stimulators to minimize tissue damage and extend battery life.
Two-photon-absorption-induced luminescence in organic waveguide couplers (1995)

We report the enhancement of two-photon-absorption-induced luminescence of a polymer film when a guided mode of the film is excited at the pump frequency, with a diffraction grating as optical coupler. The results show the double excitation of a guided mode at the pump frequency and of other guided modes at the luminescence broadband frequency. We also present experimental results of second-harmonic generation with such a device in which the effects of both absorption and diffraction are observed.
P1‐189: Conversion to dementia over a five‐year period among patients with mild cognitive impairment in a Polish follow‐up study (2008)

Mild cognitive impairment (MCI) patients can develop demonstrable cognitive impairment, without crossing the threshold for dementia. They have an increased risk of developing dementia, however not all convert to dementia, and some can revert to normal. The longitudinal study consisted 105 individuals with the diagnosis of MCI according to Peterson's et al. criteria. All subjects received annual clinical and psychometric examinations for up to mean 5 years. Diagnosis were made for dementia according to DSM III-R. All patients were given routine laboratory tests, APOE genotype, CT, (1) H MRS, SPECT and standard neuropsychological examinations. 42 subjects were classified as amnestic MCI and 63 as multi-domain MCI. Conversion to dementia over the average of 5 years period was determined. Over the follow-up period 35.3% (37/105) showed cognitive decline not dementia, 27.6% (29/105) were stabile, while 7.6% patients (8/105) showed cognitive improvement. Thirty-one out of 105 patients with MCI (29.5%) converted to dementia: 84% were diagnosed as Alzheimer's disease (AD), 3% as fronto-temporal dementia, 13% as mixed AD/vascular dementia. Patients with multi-domain MCI were more likely to convert to dementia than those with amnestic MCI. We conclude that conversion rate from MCI to dementia was 29.5% over a 5 years period. Patients with multi-domain MCI were at higher risk for more rapid conversion to clinical dementia.