gebhart/patrepeval huggingface dataset:
A Novel RFID-Based Sensing Method for Low-Cost Bolt Loosening Monitoring (2016)

In coal mines, bolt loosening in the cage guide is affected by the harsh environmental factors and cage hoist vibration, leading to significant threats to work safety. It is crucial, to this effect, to successfully detect the status of multipoint bolts of guide structures. This paper proposes a system to monitor bolt status in harsh environments established based on the RFID technique. A proof-of-concept model was demonstrated consisting of a bolt gearing system, passive UHF RFID tags, a reader, and monitoring software. A tinfoil metal film is fixed on the retaining plate and an RFID tag bonded to a large gear, with the bolt to be detected fixed in the center of a smaller gear. The radio-frequency signal cannot be received by the reader if the tag is completely obscured by the tinfoil, and if the bolt is loose, the tag's antenna is exposed when the gear revolves. A radio-frequency signal that carries corresponding bolt's information is transmitted by the RFID tag to the RFID reader due to coil coupling, identifying loose bolt location and reporting them in the software. Confirmatory test results revealed that the system indeed successfully detects bolt loosening and comparative test results (based on a reed switch multipoint bolt loosening monitor system) provided valuable information regarding the strengths and weaknesses of the proposed system.
Cholesterol Derivative of a New Triantennary Cluster Galactoside Lowers Serum Cholesterol Levels and Enhances Secretion of Bile Acids in the Rat (1995)

We conclude that TG(20A)C efficiently directs lipoproteins that contain cholesterol to the liver at a 30-fold-lower concentration than previously developed cholesterol-derived cluster galactosides. This newly developed approach to lower cholesterol levels may prove valuable for familial hypercholesterolemic patients or those with familial defective apolipoprotein B-100 who do not respond or who respond insufficiently, respectively, to conventional therapies.
Nucleosides. XI. 2',3'-Dideoxycytidine (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleosides. XI. 2',3'-DideoxycytidineJerome P. Horwitz, Jonathan Chua, Michael Noel, and Joseph T. DonattiCite this: J. Org. Chem. 1967, 32, 3, 817–818Publication Date (Print):March 1, 1967Publication History Published online1 May 2002Published inissue 1 March 1967https://doi.org/10.1021/jo01278a070RIGHTS & PERMISSIONSArticle Views242Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (318 KB) Get e-Alertsclose Get e-Alerts
Development of ytterbium-doped oxyfluoride glasses for laser cooling applications (2016)

Oxyfluoride glasses doped with 2, 5, 8, 12, 16 and 20 mol% of ytterbium (Yb(3+)) ions have been prepared by the conventional melt-quenching technique. Their optical, thermal and thermo-mechanical properties were characterized. Luminescence intensity at 1020 nm under laser excitation at 920 nm decreases with increasing Yb(3+) concentration, suggesting a decrease in the photoluminescence quantum yield (PLQY). The PLQY of the samples was measured with an integrating sphere using an absolute method. The highest PLQY was found to be 0.99(11) for the 2 mol% Yb(3+): glass and decreases with increasing Yb(3+) concentration. The mean fluorescence wavelength and background absorption of the samples were also evaluated. Upconversion luminescence under 975 nm laser excitation was observed and attributed to the presence of Tm(3+) and Er(3+) ions which exist as impurity traces with YbF3 starting powder. Decay curves for the Yb(3+): (2)F5/2 → (2)F7/2 transition exhibit single exponential behavior for all the samples, although lifetime decrease was observed for the excited level of Yb(3+) with increasing Yb(3+) concentration. Also observed are an increase in the PLQY and a slight decrease in lifetime with increasing the pump power. Finally, the potential of these oxyfluoride glasses with high PLQY and low background absorption for laser cooling applications is discussed.
The use of antiidiotypic antibodies as vaccines against infectious agents. (1987)

The development of the antibodies to the combining site of an antigen-binding antibody has been documented in humans as well as experimental animals. These antibodies have been shown in experimental models to have an important regulatory role in their ability to affect both B- and T-cell responses to antigen. Recently, with the development of technology for cloning T cells, it has been possible to produce monoclonal antibodies to the T-cell receptor proteins. These T-cell antiidiotypic antibodies have been shown to activate both B- and T-cell responses. For this reason, such reagents have the potential to be used as nonantigen-containing immunogens. Our work has demonstrated that such monoclonal anti-T-cell antiidiotypes are capable of stimulating immunity to lethal viral infections. The implications of the use of antiidiotypes against infectious organisms are discussed.
Interaction of Cytosolic Adaptor Proteins with Neuronal Apolipoprotein E Receptors and the Amyloid Precursor Protein (1998)

Apolipoprotein E, alpha2-macroglobulin, and amyloid precursor protein (APP) are involved in the development of Alzheimer's disease. All three proteins are ligands for the low density lipoprotein (LDL) receptor-related protein (LRP), an abundant neuronal surface receptor that has also been genetically linked to Alzheimer's disease. The cytoplasmic tails of LRP and other members of the LDL receptor gene family contain NPxY motifs that are required for receptor endocytosis. To investigate whether these receptors may have functions that go beyond ligand internalization, e.g. possible roles in cellular signaling, we searched for proteins that might interact with the cytoplasmic tails of the receptors. A family of adaptor proteins containing protein interaction domains that can interact with NPxY motifs has previously been described. Using yeast 2-hybrid and protein coprecipitation approaches in vitro, we show that the neuronal adaptor proteins FE65 and mammalian Disabled bind to the cytoplasmic tails of LRP, LDL receptor, and APP, where they can potentially serve as molecular scaffolds for the assembly of cytosolic multiprotein complexes. FE65 contains two distinct protein interaction domains that interact with LRP and APP, respectively, raising the possibility that LRP can modulate the intracellular trafficking of APP. Tyrosine-phosphorylated mammalian Disabled can recruit nonreceptor tyrosine kinases, such as src and abl, to the cytoplasmic tails of the receptors to which it binds, suggesting a molecular pathway by which receptor/ligand interaction on the cell surface could generate an intracellular signal.
Thymic humoral factor .gamma.2: purification and amino acid sequence of an immunoregulatory peptide from calf thymus (1988)

Thymic humoral factor gamma 2 (THF-gamma 2), an octapeptide essential for immune regulation, was purified from calf thymus. The purification of THF-gamma 2, monitored in vitro and in vivo in mouse splenocyte proliferation assays, was achieved by gel filtration of low molecular weight thymus extracts followed by ion-exchange chromatography and sequential reversed-phase high-performance liquid chromatography. The process yielded 5 micrograms of THF-gamma 2/1000 kg of thymus tissue. The concentration of THF-gamma 2 required for augmentation of lymphocyte proliferation and interleukin 2 production was 5 ng/mL in vitro and 10 ng/kg per mouse in vivo. THF-gamma 2 has the amino acid sequence Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu. The proposed structure has been confirmed because a peptide was synthesized on the basis of this sequence that showed activity identical with that of the biological molecule. It shows no homology to the amino acid sequence of other thymic hormones nor is it part of any peptide or protein of known sequence. THF-gamma 2 retains essentially all of the biological activity of the thymus extract from which it is derived.
A polymeric drug delivery system for the simultaneous delivery of drugs activatable by enzymes and/or light (1994)

Three water soluble copolymers based on N-(2-hydroxypropyl)methacrylamide were prepared. Copolymer I contains adriamycin, a chemotherapeutic agent, attached via enzymatically degradable oligopeptide (glycylphenylalanylleucylglycine; G-F-L-G) side chains. The other two copolymers contained the photosensitizer, meso-chlorin e6 monoethylene diamine disodium salt (Mce6). In Copolymer II, the chlorin is attached via the degradable G-F-L-G sequence, and it was bound by the nondegradable glycyl spacer in Copolymer III. Initially, the copolymers were characterized separately in vitro and in vivo. Combinations of the copolymer bound chemotherapeutic agent and each of the copolymer bound photosensitizers were then assessed for antitumor effect in vivo. Localization/retention studies (A/J mice; Neuro 2A neuroblastoma solid tumor) were performed with the two copolymers containing Mce6 as well as the free drug. Results of these experiments demonstrated a very different tumor uptake profile for the two copolymers. While the free drug was rapidly cleared from tumor tissue, the copolymer containing Mce6 attached via the non-degradable bond was retained for an extended period; drug concentrations in the tumor were high even after 5 days. On the other hand, a high concentration of the copolymer containing Mce6 bound via the degradable sequence was taken up by the tumor, yet its concentration in the tumor was substantially diminished at 48 h after administration. This shows indirect evidence of in vivo cleavage of Mce6 from the copolymer in the lysosomal compartment which is supported by direct evidence of cleavage by cathepsin B (a lysosomal enzyme) in vitro. Antitumor effects were assessed on Neuro 2A neuroblastoma induced in A/J mice for all three copolymers. Photodynamic therapy (PDT) proved the copolymer with Mce6 bound via the degradable oligopeptide sequence to be a more effective photosensitizer in vivo than the other chlorin containing copolymer. The difference in activity was consistent with the results obtained by photophysical analyses in which the free drug had a higher quantum yield of singlet oxygen generation than the polymer bound drug in buffer. The quantum yield of singlet oxygen generation increased with the enzymatic cleavage of the chlorin from the copolymer. Conditions were subsequently determined for which chemotherapy or PDT would show some antitumor effect, yet be incapable of curing tumors. Finally, combination therapy experiments were performed in which the copolymer bound adriamycin was mixed with either of the copolymer bound chlorin compounds and injected intravenously (i.v.) into the tail veins of mice.(ABSTRACT TRUNCATED AT 400 WORDS)
Celiac Disease (2012)

The diagnosis of celiac disease involves serologic testing (generally for IgA anti–tissue transglutaminase antibodies first) followed by upper endoscopy with biopsy for confirmation in most patients. Patients with celiac disease should follow a lifelong, strict gluten-free diet.
Definitive role for natriuretic peptide receptor-C in mediating the vasorelaxant activity of C-type natriuretic peptide and endothelium-derived hyperpolarising factor (2007)

These data substantiate CNP/NPR-C signalling as a fundamental pathway underlying EDHF-dependent regulation of vascular tone in the rat mesenteric resistance vasculature. An increased understanding of the physiological roles of CNP/NPR-C signalling in the vasculature (now facilitated by the identification of a selective NPR-C antagonist) should aid determination of the (patho)physiological importance of EDHF and might provide the rationale for the design of novel therapeutics.
Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice (2014)

The Cas9/sgRNA of the CRISPR/Cas system has emerged as a robust technology for targeted gene editing in various organisms, including plants, where Cas9/sgRNA-mediated small deletions/insertions at single cleavage sites have been reported in transient and stable transformations, although genetic transmission of edits has been reported only in Arabidopsis and rice. Large chromosomal excision between two remote nuclease-targeted loci has been reported only in a few non-plant species. Here we report in rice Cas9/sgRNA-induced large chromosomal segment deletions, the inheritance of genome edits in multiple generations and construction of a set of facile vectors for high-efficiency, multiplex gene targeting. Four sugar efflux transporter genes were modified in rice at high efficiency; the most efficient system yielding 87-100% editing in T0 transgenic plants, all with di-allelic edits. Furthermore, genetic crosses segregating Cas9/sgRNA transgenes away from edited genes yielded several genome-edited but transgene-free rice plants. We also demonstrated proof-of-efficiency of Cas9/sgRNAs in producing large chromosomal deletions (115-245 kb) involving three different clusters of genes in rice protoplasts and verification of deletions of two clusters in regenerated T0 generation plants. Together, these data demonstrate the power of our Cas9/sgRNA platform for targeted gene/genome editing in rice and other crops, enabling both basic research and agricultural applications.
A preliminary study on the immune responses during allografting of rabbit bone marrow stromal cells (2003)

To search for the ability of rabbit bone marrow stromal cells to induce the differentiation into osteoblasts and the possibility of allografting osteoblasts,the bone marrow cells were obtained fron tibia of newborne rabbits and cultivated in vitro in conditional DMEM medium with 10% of fetal bovine serum After 5 generations of passages,the cells were examined with Wrights staining,alkaline phosphatase (ALP) staining,and red alizarin staining Several pieces of the gelfoam absorbed revived cells were transplanted subcutaneously and intramuscularly Speciemens were observed after 2,4,8 and 12 weeks of operation,and the ability to form osteoblasts as well as the allograft rejection reaction were analyzed The results showed that the bone marrow osteoblasts of the in vitro induced cultures were a comparatively pure cell lines with spindle shape and with several protuberances,and to be positive in the ALP staining After cultivation in vitro for 40 days,many mineralized nodes were found around the cultured cells Majority of the transplanted osteoblasts were survived and after 4 weeks the transplanted cells began to produce osteoid matrix with irregular mineralized bone tissue Around the transplanted cells,there was only small amount of lymphocyte or eosinphil infiltrations It concludes that BMSC have significantly osteogenic potential and the transplanted cells still perserve their fundamental biological functions with slight degree of rejection reactions These results suggest it is possible for the performance of cell allografting
ID ‐ A dominant negative regulator of skeletal muscle differentiation — is not involved in maturation or differentiation of vascular smooth muscle cells (1995)

Rat aortic vascular smooth muscle cells (VSMCs) expressing the MyoD gene formed myotubes at a low frequency in the presence of serum but at a high frequency in the absence of serum. Expression of an antisense Id gene increased myotube formation in the presence of serum indicating that a reduction in Id levels is a major mechanism by which serum withdrawal promotes myotube formation. The role of Id in the development of VSMCs was investigated by expressing an antisense Id gene in neonatal VSMCs. No evidence was found for the conversion of neonatal VSMCs to adult VSMCs in the presence of the antisense Id gene. Similarly reduction in Id by serum withdrawal also failed to cause conversion of the neonatal VSMCs to the adult phenotype. These data suggest that the maturation of neonatal smooth muscle cells is not controlled by a VSMC homologue of the skeletal muscle basic-helix-loop-helix proteins.
Biological Role and Potential Therapeutic Targeting of the Chemokine Receptor CXCR4 in Undifferentiated Thyroid Cancer (2007)

Anaplastic thyroid carcinoma (ATC) is a rare thyroid cancer type with an extremely poor prognosis. Despite appropriate treatment, which includes surgery, radiotherapy, and chemotherapy, this cancer is invariably fatal. CXCR4 is the receptor for the stromal cell-derived factor-1 (SDF-1)/CXCL12 chemokine and it is expressed in a variety of solid tumors, including papillary thyroid carcinoma. Here, we show that ATC cell lines overexpress CXCR4, both at the level of mRNA and protein. Furthermore, we found that CXCR4 was overexpressed in ATC clinical samples, with respect to normal thyroid tissues by real-time PCR and immunohistochemistry. Treatment of ATC cells with SDF-1 induced proliferation and increase in phosphorylation of extracellular signal-regulated kinases and protein kinase B/AKT. These effects were blocked by the specific CXCR4 antagonist AMD3100 and by CXCR4 RNA interference. Moreover, AMD3100 effectively reduced tumor growth in nude mice inoculated with different ATC cells. Thus, we suggest that CXCR4 targeting is a novel potential strategy in the treatment of human ATC.
Stimulation of muscle growth by clenbuterol: lack of effect on muscle protein biosynthesis (1986)

1. Young rats were offered to appetite a semi-synthetic diet either alone or containing the beta 2-selective agonist clenbuterol (4-amino-alpha[t-butylamino)methyl]-3,5-dichlorobenzyl alcohol). 2. In female rats (starting weight 116g) the presence of the drug at daily doses greater than 10 micrograms/kg body-weight per d increased the growth of skeletal and cardiac muscle but had no stimulatory effect on the growth of the liver, gastrointestinal tract and kidney. 3. Male rats (starting weight 53 g) received clenbuterol at a daily oral dose of 200 micrograms/kg body-weight per d. Animals were slaughtered after 0, 4, 8, 11, 18, 21 and 25 d of treatment. At 4, 11, 21 and 25 d muscle protein synthesis was measured by the method of Garlick et al. (1980). Although clenbuterol increased the rate of protein and RNA accretion in gastrocnemius and soleus muscles, protein synthesis was not increased. 4. The results suggested that the drug had a rapid, perhaps direct, inhibitory effect on protein degradation. It is concluded that the growth-promoting effect of clenbuterol may be specific to muscle and that the drug may act in a novel manner which circumvents the physiological mechanisms responsible for the control of muscle growth.
Marshall-Marchetti Procedure: Modification (1966)

No AccessJournal of Urology1 Nov 1966Marshall-Marchetti Procedure: Modification Charles F. Mckiel, Edwin C. Graf, and Daniel H. Callahan Charles F. MckielCharles F. Mckiel , Edwin C. GrafEdwin C. Graf , and Daniel H. CallahanDaniel H. Callahan View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)63342-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1966 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited by Enderev T (1992) Anchor fixation and other modifications of endoscopic bladder neck suspensionUrology, 10.1016/0090-4295(92)90453-4, VOL. 40, NO. 5, (409-418), Online publication date: 1-Nov-1992. Winter C (1982) Peripubic urethropexy for urinary stress incontinence in womenUrology, 10.1016/0090-4295(82)90466-6, VOL. 20, NO. 4, (408-411), Online publication date: 1-Oct-1982. Uhle C, Kohler F and Mackinney C (2018) Urinary Stress Incontinence in the Female PatientJournal of Urology, VOL. 99, NO. 5, (613-616), Online publication date: 1-May-1968. Volume 96Issue 5November 1966Page: 737-739 Advertisement Copyright & Permissions© 1966 by The American Urological Association Education and Research, Inc.Metrics Author Information Charles F. Mckiel More articles by this author Edwin C. Graf More articles by this author Daniel H. Callahan More articles by this author Expand All Advertisement PDF downloadLoading ...
Antiretroviral and immune-based therapies (1993)

From the Centre Hospitaller Universitaire Bichat–Claude Bernard, Paris, France *University of Colorado Health Sciences Center, Denver, Colorado †New England Deaconess Hospital and Harvard Medical School, Boston, Massachusetts, USA
In vivo microdialysis and immunohistochemical analyses of tendon tissue demonstrated high amounts of free glutamate and glutamate NMDAR1 receptors, but no signs of inflammation, in Jumper's knee (2001)

This investigation describes, to our knowledge, the first experiment where the microdialysis technique was used to study certain metabolic events in human patellar tendons in combination with immunohistochemical analyses of tendon biopsies. In five patients (four men and one woman) with a long duration (range 12-36 months) of pain symptoms from Jumper's knee (localized tenderness in the patellar tendon verified as tendon changes with ultrasonography or MRI), and in five controls (four men and one woman) with normal patellar tendons, a standard microdialysis catheter was inserted into the patellar tendon under local anestesia. The local concentrations of glutamate (excitatory neurotransmitter) and prostaglandin E2 (PGE2) were registered under resting conditions. Samplings were done every 15 min during a 2 h period. In all individuals (patients and controls) biopsies were taken for immunohistochemical analyses. The results showed that it was possible to detect and measure the concentrations of glutamate and PGE2 in the patellar tendon with the use of microdialysis technique. There were significantly higher concentrations of free glutamate, but not PGE2, in tendons with tendinosis compared to normal tendons. In the biopsies, there were no inflammatory cell infiltrates, but, for the first time, it was shown that there was immunoreaction for the glutamate receptor NMDAR1 in association with nerve structures in human patellar tendons. These findings altogether indicate that glutamate might be involved in painful Jumper's knee, and further emphasizes that there is no chemical inflammation (normal PGE2 levels) in this chronic condition.