gebhart/patrepeval huggingface dataset:
Angiotensin-converting enzyme 2, angiotensin-(1–7) and Mas: new players of the renin–angiotensin system (2012)

Angiotensin (Ang)-(1-7) is now recognized as a biologically active component of the renin-angiotensin system (RAS). Ang-(1-7) appears to play a central role in the RAS because it exerts a vast array of actions, many of them opposite to those attributed to the main effector peptide of the RAS, Ang II. The discovery of the Ang-converting enzyme (ACE) homolog ACE2 brought to light an important metabolic pathway responsible for Ang-(1-7) synthesis. This enzyme can form Ang-(1-7) from Ang II or less efficiently through hydrolysis of Ang I to Ang-(1-9) with subsequent Ang-(1-7) formation by ACE. In addition, it is now well established that the G protein-coupled receptor Mas is a functional binding site for Ang-(1-7). Thus, the axis formed by ACE2/Ang-(1-7)/Mas appears to represent an endogenous counterregulatory pathway within the RAS, the actions of which are in opposition to the vasoconstrictor/proliferative arm of the RAS consisting of ACE, Ang II, and AT(1) receptor. In this brief review, we will discuss recent findings related to the biological role of the ACE2/Ang-(1-7)/Mas arm in the cardiovascular and renal systems, as well as in metabolism. In addition, we will highlight the potential interactions of Ang-(1-7) and Mas with AT(1) and AT(2) receptors.
The metabolism of sphingomyelin. II. Evidence of an enzymatic deficiency in Niemann-Pick diseae. (1966)

The accumulation of excessive quantities of sphingomyelin in tissues of patients with Niemann-Pick disease was demonstrated by Klenk in 19341, 2 and has been amply confirmed by other investigators.3-5A study by Crocker and Mays6 in- dicated that the rate of biosynthesis of sphingomyelin in tissues from these patients appeared to be essentially normal.These findings suggested that the metabolic lesion in this condition might be of a catabolic nature.We have recently obtained evidence for the presence of a specific enzyme in liver tissue which catalyzes the hydrolysis of sphingomyelin.7 The enzyme was partially purified from rat liver tissue, and the products of the reaction were demonstrated to be phosphorylcholine and ceramide.The most highly purified enzyme preparations did not catalyze the hydrolysis of lecithin or phosphatidylethanolamine.Lecithin, however, appeared to be a competitive inhibitor of the reaction.Similar enzymatic activity could be detected in human liver tissue.The present report describes experiments in which the level of the sphingomyelin-cleaving enzyme in liver and kidney tissue obtained from six patients with the classic infantile form of Niemann- Pick disease8 is compared with the values found in a liver biopsy sample from a normal human adult and liver and kidney tissue of patients with various other dis- orders.Materials and Methods.-Sphingomyelinlabeled with C14 in the methyl carbon atoms of the choline portion of the molecule was synthesized as described previously.7Samples of liver and kidney tissue were homogenized in 10 vol of 0.25 M sucrose with an all-glass TenBroeck homoge- nizer and centrifuged at 600 X g for 12 min.Suitable aliquots of the supernatant suspensions were
Pancreas transplantation: a treatment option for insulin-dependent diabetes mellitus. (1996)

The success with which pancreas transplantation normalizes glucose concentration in patients with Type I diabetes mellitus has made it an important treatment option. Most pancreas transplant operations are performed in combination with a kidney transplant, and neither overall patient survival nor renal graft survival is compromised by the addition of a pancreas graft. Pancreas after kidney transplantation and isolated pancreas transplantation are performed less frequently since the pancreas graft success rate remains lower in these operations compared to combined pancreas-kidney transplantation. Pancreas transplantation improves the quality of life and stabilizes or reverses some diabetic microvascular complications, but its impact on the risk of atherosclerotic vascular disease is still unknown. The relative risks and benefits of pancreas transplantation need to be carefully assessed for each candidate through a thorough screening process, regardless of which type of graft is being considered. However, patient counseling and selection will be greatly aided by further research assessing the long-term risks and benefits of all types of pancreas transplantation. Pancreas transplantation will probably remain an important treatment option for some patients with Type I diabetes mellitus until this disease can either be successfully prevented or alternative treatment strategies are developed that provide equal glycaemic control with less or no associated immunosuppression.
A Fruiting Body Tissue Method for Efficient Agrobacterium -Mediated Transformation of Agaricus bisporus (2000)

We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.
Current Status and Research Strategies in Tuberculosis Drug Development (2011)

ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTADDITION / CORRECTIONThis article has been corrected. View the notice.Current Status and Research Strategies in Tuberculosis Drug DevelopmentMiniperspectiveLynn G. Dover‡ and Geoffrey D. Coxon*†View Author Information† Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom‡ Biomolecular and Biomedical Research Centre, School of Life Sciences, Northumbria University, Newcastle upon Tyne, United KingdomPhone: +44(0)141 5485754. E-mail: [email protected]Cite this: J. Med. Chem. 2011, 54, 18, 6157–6165Publication Date (Web):August 8, 2011Publication History Received17 March 2011Published online16 August 2011Published inissue 22 September 2011https://pubs.acs.org/doi/10.1021/jm200305qhttps://doi.org/10.1021/jm200305qreview-articleACS PublicationsCopyright © 2011 American Chemical SocietyRequest reuse permissionsArticle Views6651Altmetric-Citations97LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Antimicrobial agents,Bacteria,Drug discovery,Infectious diseases,Pharmaceuticals Get e-Alerts
Near-Online Multi-target Tracking with Aggregated Local Flow Descriptor (2015)

In this paper, we tackle two key aspects of multiple target tracking problem: 1) designing an accurate affinity measure to associate detections and 2) implementing an efficient and accurate (near) online multiple target tracking algorithm. As for the first contribution, we introduce a novel Aggregated Local Flow Descriptor (ALFD) that encodes the relative motion pattern between a pair of temporally distant detections using long term interest point trajectories (IPTs). Leveraging on the IPTs, the ALFD provides a robust affinity measure for estimating the likelihood of matching detections regardless of the application scenarios. As for another contribution, we present a Near-Online Multi-target Tracking (NOMT) algorithm. The tracking problem is formulated as a data-association between targets and detections in a temporal window, that is performed repeatedly at every frame. While being efficient, NOMT achieves robustness via integrating multiple cues including ALFD metric, target dynamics, appearance similarity, and long term trajectory regularization into the model. Our ablative analysis verifies the superiority of the ALFD metric over the other conventional affinity metrics. We run a comprehensive experimental evaluation on two challenging tracking datasets, KITTI [16] and MOT [2] datasets. The NOMT method combined with ALFD metric achieves the best accuracy in both datasets with significant margins (about 10% higher MOTA) over the state-of-the-art.
New discretization procedure for the breakage equation (1995)

Abstract A new discretization method, applicable for both batch and continuous systems, is developed for the breakage equation. The problem of intrainterval interactions due to discretization is accounted for by matching the zeroth and first moments of the continuous population balance equation with the corresponding moments of the discretized equation, thereby guaranteeing conservation of mass and total number of particles. Without loss of generality, the use of this method is demonstrated with a power law form of the specific rate of breakage, and with both theoretical and empirical breakage functions. The systematic method requires minimum computational efforts by allowing the user to choose either geometric size intervals with any geometric ratio or equal‐size intervals for the particle size range. Simulation results show that the new method significantly improves predictions of the particle size distribution over the discretization method currently in use.
Suppression of lung cancer tumor growth in a nude mouse model by the Ras inhibitor salirasib (farnesylthiosalicylic acid) (2007)

Aberrant Ras pathway functions contribute to the malignant phenotype of lung cancers. Inhibitors of Ras might therefore be considered as potential drugs for lung cancer therapy. Here, we show that the Ras inhibitor farnesylthiosalicylic acid (salirasib) inhibits proliferation of human lung cancer cells harboring a mutated K-ras gene (A549, H23, or HTB54) or overexpressing a growth factor receptor (H1299 or HTB58) and enhances the cytotoxic effect of the chemotherapeutic drug gemcitabine. Salirasib inhibited active K-Ras in A549 cells, reversed their transformed morphology, and inhibited their anchorage-independent growth in vitro. Tumor growth in A549 and HTB58 cell nude mouse models was inhibited by i.p. administration of salirasib. P.o. formulated salirasib also inhibited A549 cell tumor growth. Our results suggest that p.o. salirasib may be considered as a potential treatment for lung cancer therapy.
Invasion of HEp-2 cells by strains of Salmonella typhimurium of different virulence in relation to gastroenteritis (1991)

Experiments to measure the invasiveness of seven strains of Salmonella typhimurium for HEp-2 cells showed that high inocula (100 bacteria/HEp-2 cell), as used by most workers to synchronise events and to increase the number of bacteria which invade, resulted in recovery of significantly less than 1% of the original inoculum after treatment with gentamicin to kill extracellular bacteria. Also, the cell culture medium became acidic, and microscopic examination of Giemsa-stained monolayers immediately following gentamicin treatment revealed high concentrations of bacteria associated with the cells. Moreover, with bacterium-cell interaction beyond 2 h, many HEp-2 cells became rounded, especially with virulent strains W118 and TML. Thus, the biological significance of the quantitative data was uncertain. The fall in pH and the rounding of HEp-2 cells were prevented by the use of a low (1:1) bacterium: cell ratio; but the recovery of bacteria after treatment with gentamicin was still lower than expected by microscopic examination. After treatment of cells with Triton X-100 to release bacteria, many remained bound to residual cell nuclei. Additional treatment with a rubber policeman, and vigorous pipetting to disperse aggregates of bacteria and cell debris, increased the recovery to c. 10% of the initial inoculum after interaction for 2 h, and 30-80% after 4 h, depending on the strain and experimental conditions. The pattern of invasiveness, but not the absolute count, was highly reproducible on different days and in different hands. However, after interaction exceeding 2 h, the distribution of bacteria was uneven, many cells having no associated organisms, others showing microcolonies.(ABSTRACT TRUNCATED AT 250 WORDS)
Incision at hypoxanthine residues in DNA by a mammalian homologue of the Escherichia coli antimutator enzyme endonuclease V (2003)

Deamination of DNA bases can occur spontaneously, generating highly mutagenic lesions such as uracil and hypoxanthine. In Escherichia coli two enzymes initiate repair at hypoxanthine residues in DNA. The alkylbase DNA glycosylase, AlkA, initiates repair by removal of the damaged base, whereas endonuclease V, Endo V, hydrolyses the second phosphodiester bond 3' to the lesion. We have identified and characterised a mouse cDNA with striking homology to the E.coli nfi gene, which also has significant similarities to motifs required for catalytic activity of the UvrC endonuclease. The 37-kDa mouse enzyme (mEndo V) incises the DNA strand at the second phosphodiester bond 3' to hypoxanthine- and uracil-containing nucleotides. The activity of mEndo V is elevated on single-stranded DNA substrate in vitro. Expression of the mouse protein in a DNA repair-deficient E.coli alkA nfi strain suppresses its spontaneous mutator phenotype. We suggest that mEndo V initiates an alternative excision repair pathway for hypoxanthine removal. It thus appears that mEndo V has properties overlapping the function of alkylbase DNA glycosylase (Aag) in repair of deaminated adenine, which to some extent could explain the absence of phenotypic abnormalities associated with Aag knockout in mice.
VEGF-C receptor binding and pattern of expression with VEGFR-3 suggests a role in lymphatic vascular development (1996)

The vascular endothelial growth factor family has recently been expanded by the isolation of two new VEGF-related factors, VEGF-B and VEGF-C. The physiological functions of these factors are largely unknown. Here we report the cloning and characterization of mouse VEGF-C, which is produced as a disulfide-linked dimer of 415 amino acid residue polypeptides, sharing an 85% identity with the human VEGF-C amino acid sequence. The recombinant mouse VEGF-C protein was secreted from transfected cells as VEGFR-3 (Flt4) binding polypeptides of 30-32x10(3) Mr and 22-23x10(3) Mr which preferentially stimulated the autophosphorylation of VEGFR-3 in comparison with VEGFR-2 (KDR). In in situ hybridization, mouse VEGF-C mRNA expression was detected in mesenchymal cells of postimplantation mouse embryos, particularly in the regions where the lymphatic vessels undergo sprouting from embryonic veins, such as the perimetanephric, axillary and jugular regions. In addition, the developing mesenterium, which is rich in lymphatic vessels, showed strong VEGF-C expression. VEGF-C was also highly expressed in adult mouse lung, heart and kidney, where VEGFR-3 was also prominent. The pattern of expression of VEGF-C in relation to its major receptor VEGFR-3 during the sprouting of the lymphatic endothelium in embryos suggests a paracrine mode of action and that one of the functions of VEGF-C may be in the regulation of angiogenesis of the lymphatic vasculature.
Production of genetically modified lysozymes having extreme heat stability and antimicrobial activity against Gram negative bacteria in yeast and in plant (1998)

Hen egg white lysozyme was genetically modified to have extreme heat stability and strong antimicrobial activity against Gram negative bacteria and the modified lysozymes were secreted in yeast and tobacco. Complementary DNA encoding lysozyme was subjected to site-directed mutagenesis to have the Asn-X-Thr(Ser) sequence that is the signal for asparagine-linked glycosylation at the positions 49. The glycosyl lysozyme enhanced heat stability was expressed in the yeast carrying the modified lysozyme cDNA. The expression amount of glycosyl lysozyme was about 10 mg/l of yeast culture medium. Using the same yeast expression system, the lysozyme enhanced antimicrobial action by inserting hydrophobic penta-peptide at the C-terminus were secreted in a small amount (less than 100 micrograms/l in the yeast culture medium). These cDNA constructs of modified lysozymes were engineered into tabacco through Agrobacterium-mediated transformation in order to construct antimicrobial plant. The expression of lysozymes was confirmed by the reverse transcriptional PCR, SDS-PAGE analysis and lytic activity of transformants of tobacco. The transformant having the highest lytic activity expressed about 40 micrograms of lysozyme per g of leaf tissue.
Antineoplastic effects in rats of 5-fluorocytosine in combination with cytosine deaminase capsules. (1985)

5-Fluorocytosine (5-FC) lacks antineoplastic activity in human subjects because of the absence of cytosine deaminase (CDase) in mammalian cells. Intratumoral conversion of 5-FC into 5-fluorouracil (5-FUra) by locally implanted capsules containing CDase followed by systemic administration of 5-FC can be expected to induce antineoplastic activity at a local site with minimal systemic toxicity. In vitro and in vivo experiments were performed to evaluate this hypothesis. Spectrophotometric analysis confirmed the deamination of 5-FC to 5-FUra by CDase extracted from cultivated Escherichia coli. In vitro studies showed that 5-FC combined with CDase induced significant growth-inhibitory effects on the cultured glioma cells. An active CDase capsule, made of cellulose tubing, was newly designed for local implantation. 5-FC concentrations in the s.c. tumors of the rats given these CDase capsules, followed by 5-FC administration, showed a sufficient amount of delivery of 5-FC to the tumor tissue. 5-FUra appearing in the tumor reached the level of 8.0 micrograms/g at 2 h and stayed at more than 1.0 microgram/g at between 1 and 6 h. Significant reduction of the tumor growth and cytotoxic changes were observed. The passive cutaneous anaphylaxis reaction demonstrated no allergic reaction to the host due to the capsule. These results suggest that this chemotherapeutic method is effective for human brain tumors.
Human Apolipoprotein E Receptor 2 (1996)

Isolation and characterization of a human cDNA demonstrated a novel lipoprotein receptor designated apolipoprotein E receptor 2 (apoER2). The new receptor consists of five functional domains resembling the low density lipoprotein (LDL) and very low density lipoprotein (VLDL) receptors. LDL receptor deficient Chinese hamster ovary cells expressing human apoER2 bound apoE rich beta-migrating VLDL with high affinity and internalized. LDL was bound with much lower affinity to these cells. The 4.5- and 8.5-kb mRNAs for the receptor were most highly expressed in human brain and placenta. In rabbit tissues, multiple species of the mRNA with 4, 4.5, 5.5, 8.5, and 11 kb were detected most intensely in brain and testis and, to a much lesser extent, in ovary, but were undetectable in other tissues. In rat adrenal pheochromocytoma PC12 cells, the receptor mRNA was induced by treatment of the cells with nerve growth factor. The receptor transcripts were detectable most intensely in the cerebellar cortex, choroid plexus, ependyma, hippocampus, olfactory bulb and, to a much lesser extent, in the cerebral cortex as revealed by in situ hybridization histochemistry. In the cerebellar cortex, the receptor transcripts were densely deposited in Purkinje cell somata.
A seizure warning system for long-term epilepsy monitoring (1995)

We developed a system to provide a warning early in the development of a seizure with a reasonably low false alarm rate. Such a warning will improve the close observation of seizures and interaction between observers and patients early in the seizure, even in seizures having no obvious clinical manifestation. The system relies on the availability of the EEG recording of one sample seizure, which is used as a template for subsequent detection. We evaluated the performance in 24 seizure types from scalp and intracerebral recordings. It yielded a 100% detection rate and a false alarm rate averaging one false alarm every 5 hours. The warning signal was given, on average, 9.6 seconds after EEG seizure onset. The system will be useful in improving the clinical observation of seizures and may allow ictal SPECT scans to be more widely performed.
The site of antiviral action of 3-nitrosobenzamide on the infectivity process of human immunodeficiency virus in human lymphocytes. (1993)

The C-nitroso compound 3-nitrosobenzamide, which has been shown to remove zinc from the retroviral-type zinc finger of p7NC nucleocapsid proteins, inhibits acute infection of human immunodeficiency virus type 1 in cultured human lymphocytes. The attachment of the virus to lymphocytes and the activities of critical viral enzymes, such as reverse transcriptase, protease, and integrase, are not affected by 3-nitrosobenzamide. However, the process of reverse transcription to form proviral DNA is effectively abolished by the drug, identifying the mode of action of 3-nitrosobenzamide as interrupting the role of p7NC in accurate proviral DNA synthesis during the infectious phase of the virus life cycle.
Dynamic threshold-voltage MOSFET (DTMOS) for ultra-low voltage VLSI (1997)

In this paper, we propose a novel operation of a MOSFET that is suitable for ultra-low voltage (0.6 V and below) VLSI circuits. Experimental demonstration was carried out in a Silicon-On-Insulator (SOI) technology. In this device, the threshold voltage of the device is a function of its gate voltage, i.e., as the gate voltage increases the threshold voltage (V/sub t/) drops resulting in a much higher current drive than standard MOSFET for low-power supply voltages. On the other hand, V/sub t/ is high at V/sub gs/=0, therefore the leakage current is low. We provide extensive experimental results and two-dimensional (2-D) device and mixed-mode simulations to analyze this device and compare its performance with a standard MOSFET. These results verify excellent inverter dc characteristics down to V/sub dd/=0.2 V, and good ring oscillator performance down to 0.3 V for Dynamic Threshold-Voltage MOSFET (DTMOS).