gebhart/patrepeval huggingface dataset:
Ultrasonic separation of suspended particles (2002)

The forces on suspended particles in acoustic fields are reviewed briefly and the theoretical modelling of ultrasonic separators based on piezoelectrically excited layered resonators is described. Two flow-through resonator chamber concepts for ultrasonic particle (bio-cell) separation are investigated: (a) the coagulation or sedimentation approach, (b) the so-called h-shaped ultrasonic separator. The h-shaped ultrasonic separator is analysed by combining for the first time the mathematical modelling of the laminar flow with the acoustic force based velocity field of the particles relative to the suspension medium. This allows a complete modelling of the resonator's particle separation performance. Examples for separation chamber designs optimized by use of the mathematical model are presented and the calculated particle traces in the h-resonator are shown and compared with experimental results. For direct comparison of different ultrasonic flow through separator concepts a separation performance figure is introduced and its value is given for the two investigated separator concepts for the sample suspensions of polystyrene spheres, yeast and spirulina cells in (salt) water. The presented results are of importance for the state of the art design of acoustic cell filters for perfusion type bioreactors, as recently launched at the biotechnology market, as well as for the ultrasonic separation of plant (algae) cells under low gravity conditions, where the sedimentation concept fails.
slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains. (1990)

The Drosophila slit locus encodes a protein with four regions containing tandem arrays of a 24-amino-acid leucine-rich repeat (LRR) with conserved flanking sequences (flank-LRR-flank surrounding these arrays), followed by two regions with epidermal growth factor (EGF)-like repeats. Each of these motifs has been implicated in protein-protein interactions as part of an extracellular domain in a variety of other proteins. Analysis of slit cDNA clones reveals that as a consequence of alternative splicing, the locus can code for two distinct protein species differing by 11 amino acids at the carboxyl terminus of the last EGF repeat. The existence of a putative signal sequence and the absence of a transmembrane domain suggest that slit is secreted, an observation supported by an analysis of its expression in tissue culture. Examining the expression pattern of slit in the embryo by antibody staining, enhancer trap detection, and in situ hybridization, we demonstrate that the protein is expressed by a subset of glial cells along the midline of the developing central nervous system. Through immunoelectron microscopy, slit can be seen on the commissural axons traversing the glial cells although it is absent from the cell bodies of these neurons, implying that slit is exported by the glia and distributed along the axons. Finally, we demonstrate that a reduction in slit expression results in a disruption of the developing midline cells and the commissural axon pathways. The embryonic localization, mutant phenotype, and homology of slit to both receptor-binding EGF-like ligands and adhesive glycoproteins suggest that it may be involved in interactions between the midline glial cells, their extracellular environment, and the commissural axons that cross the midline.
Memory Charging Effect in Silicon Nanoparticles of Pentacene Capacitor Device (2011)

In this study, an organic memory structure having citrate-capped silicon nanoparticles (Si NPs) self-assembled on SiO2 layer is demonstrated to show memory charging effect. The Si NPs were prepared by a simple chemical oxidation and etching method with silicon powder. The hysteresis loops on C-V measurement were obtained by flatband voltage shifts, which demonstrate the presence of charge storages in the embedded pentacene capacitor of Si NPs. The formation of Si NPs and the citrate capping on the Si NPs were analyzed by high resolution transmission electron microscopy and Fourier transform infrared spectroscopy, respectively. With voltage sweep of +/- 10 V, a hysteresis loop having flatband voltage shift of 9.9 V was obtained. In addition, electrical performance measurement for the charge storage showed more than 10,000 s charge retention time. (C) 2011 The Electrochemical Society. [DOI: 10.1149/1.3544488] All rights reserved.
Identification, functional expression and chromosomal localisation of a sustained human proton‐gated cation channel (1998)

Non-inactivating or slowly inactivating proton-gated cation channels are thought to play an important role in the perception of pain that accompanies tissue acidosis. We have identified a novel human proton-gated cation channel subunit that has biphasic desensitisation kinetics with both a rapidly inactivating Na+-selective and a sustained component. The protein shares 84% sequence identity with the proton-gated cation channel rASIC3 (rDRASIC) from rat sensory neurones. The biphasic desensitisation kinetics and the sequence homology suggest that this novel clone (hASIC3) is the human orthologue of rASIC3 (rDRASIC). While rASIC3 (rDRASIC) requires very acidic pH (pH < 4.5) for activation of the sustained current, the non-inactivating hASIC3 current starts to be activated when the pH decreases to below pH 6. hASIC3 is an acid sensor and might play an important role in the detection of lasting pH changes in human. We localised the hASIC3 gene to the human chromosome 7q35, 6.4 cRad telomeric from the microsatellite AFMA082XC9.
Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells (2009)

The pluripotency factor Nanog is expressed in peri-implantation embryos and primordial germ cells (PGCs). Nanog-deficient mouse embryos die soon after implantation. To explore the function of Nanog in germ cells, Nanog RNA was conditionally knocked down in vivo by shRNA. Nanog shRNA transgenic (NRi-Tg) mice were generated through the formation of germline chimeras with NRi-Tg embryonic stem cells. In E12.5 Cre-induced ER-Cre/NRi-Tg and TNAP-Cre/NRi-Tg double-transgenic embryos, the number of alkaline phosphatase-positive and SSEA1-positive PGCs decreased significantly. In the E9.5 and E10.5 migrating Nanog-knockdown PGCs, TUNEL-positive apoptotic cell death became prominent in vivo and in vitro, despite Oct4 expression. Single-cell microarray analysis of E10.5 Nanog-knockdown PGCs revealed significant up- and downregulation of a substantial number of genes, including Tial1, Id1 and Suz12. These data suggest that Nanog plays a key role in the proliferation and survival of migrating PGCs as a safeguard of the PGC-specific molecular network.
Rheological Properties of Silica Particle Suspensions in Mineral Oil (2005)

The rheological properties of particles suspended in a non‐polar mineral oil have been investigated as a function of volume fraction of particles, particle size, surface properties and shear rate. Three different types of particles were investigated; glass microspheres, monodisperse silica particles and fumed silica. The suspensions showed shear thinning behavior at higher volume fractions, and the viscosity increased with decreasing particle size. The hydrophobic particles display lass shear thinning effects. The relative viscosity of all the suspensions was well fitted to the Krieger and Dougherty model.
Effects of combined administration of vasopressin, epinephrine, and norepinephrine during cardiopulmonary resuscitation in pigs* (2005)

Vasopressin with or without epinephrine and norepinephrine resulted in higher myocardial and cerebral perfusion than epinephrine alone, but there was no benefit in adding norepinephrine to vasopressin and epinephrine with regard to cardiac and cerebral blood flow during cardiopulmonary resuscitation.
Inflammation and Cardiovascular Disease in RA (2006)

Patients with rheumatoid arthritis (RA) appear to have high risk for cardiovascular disease, independent of traditional risk factors. Whether
A new and efficient total synthesis of (.+-.)-longifolene (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA new and efficient total synthesis of (.+-.)-longifoleneWolfgang Oppolzer and Thierry GodelCite this: J. Am. Chem. Soc. 1978, 100, 8, 2583–2584Publication Date (Print):April 1, 1978Publication History Published online1 May 2002Published inissue 1 April 1978https://pubs.acs.org/doi/10.1021/ja00476a071https://doi.org/10.1021/ja00476a071research-articleACS PublicationsRequest reuse permissionsArticle Views2339Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Phospholipase C, protein kinase C, Ca2+/calmodulin‐dependent protein kinase II, and redox state are involved in epigallocatechin gallate‐induced phospholipase D activation in human astroglioma cells (2004)

We show that epigallocatechin-3 gallate (EGCG), a major component of green tea, stimulates phospholipase D (PLD) activity in U87 human astroglioma cells. EGCG-induced PLD activation was abolished by the phospholipase C (PLC) inhibitor and a lipase inactive PLC-gamma1 mutant, which is dependent on intracellular or extracellular Ca(2+), with the possible involvement of Ca(2+)/calmodulin-dependent protein kinase II (CaM kinase II). EGCG induced translocation of PLC-gamma1 from the cytosol to the membrane and PLC-gamma1 interaction with PLD1. EGCG regulates the activity of PLD by modulating the redox state of the cells, and antioxidants reverse this effect. Moreover, EGCG-induced PLD activation was reduced by PKC inhibitors or down-regulation of PKC. Taken together, these results show that, in human astroglioma cells, EGCG regulates PLD activity via a signaling pathway involving changes in the redox state that stimulates a PLC-gamma1 [Ins(1,4,5)P(3)-Ca(2+)]-CaM kinase II-PLD pathway and a PLC-gamma1 (diacylglycerol)-PKC-PLD pathway.
Increased stability of nucleolin in proliferating cells by inhibition of its self-cleaving activity. (1991)

Nucleolin is the major nucleolar phosphoprotein of exponentially growing eukaryotic cells and is presumably involved in pre-rRNA transcription and ribosome biogenesis. Monoclonal antibodies against nucleolin were selected by a differential dot-immunobinding assay. Nucleolin expression during T lymphocyte activation was monitored by the specific antibody. Results showed that nucleolin fluctuated in parallel to DNA synthesis. The intact 105-kDa nucleolin molecule was the major species in actively dividing cells, whereas the degraded forms were relatively abundant in nondividing cells. These results imply that stability of nucleolin molecule is cell proliferation-dependent. When affinity purified nucleolin containing undetectable contaminants was incubated at 37 degrees C, the majority of 105-kDa nucleolin was cleaved by 6 h and completely degraded within 24 h. This purified nucleolin was further separated from possible copurified protease, if any, on a reducing sodium dodecyl sulfate-polyacrylamide gel. After renaturation, the 105-kDa nucleolin immobilized in the gel was also cleaved at 37 degrees C. These data have confirmed that nucleolin protein autocatalyzes its own degradation. The self-cleaving activity of nucleolin was inhibited by nuclear extracts prepared from proliferating cells. Apparently, a proteolytic inhibitor(s) in the nuclei of proliferating cells stabilized the nucleolin molecule. It provides an unique regulatory mechanism for nucleolin expression.
Controlled formation of flavor compounds by preparation and application of Maillard reaction intermediate (MRI) derived from xylose and phenylalanine (2017)

An effective method for preparing Maillard reaction intermediate (MRI) derived from xylose (Xyl) and phenylalanine (Phe) in aqueous medium was proposed in this study.
Nota Corta: Endophytic fungi in healthy soybean leaves (2002)

Fungal endophytes were isolated from healthy cultivated soybean leaves at two growth stages (R2-R3 and R4-R5) during the growing season 1997/98. Samples were obtained from a segregating population (F3 generation) cultivated at the experimental field of the Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Buenos Aires, Argentina. Twelve genera of endophytic fungi were isolated and identified. In general, in both growth stages the same species were isolated and most of them did not show significant differences in their infection frequencies. Most of the fungi isolated are cited as soybean pathogens in different places of the world. The most commonly isolated endophytes included Alternaria alternata and Glomerella cingulata (= Colletotrichum gloeosporioides).
Membrane D-lactate dehydrogenase from Escherichia coli. Purification and properties (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMembrane D-lactate dehydrogenase from Escherichia coli. Purification and propertiesMasamitsu FutaiCite this: Biochemistry 1973, 12, 13, 2468–2474Publication Date (Print):June 1, 1973Publication History Published online1 May 2002Published inissue 1 June 1973https://pubs.acs.org/doi/10.1021/bi00737a016https://doi.org/10.1021/bi00737a016research-articleACS PublicationsRequest reuse permissionsArticle Views1007Altmetric-Citations95LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cohort Study of Structured Reporting Compared with Conventional Dictation (2009)

While there are many potential benefits of structuring radiology reports, such changes cannot be assumed to improve report accuracy or completeness. Any SRS should be tested for effect on intrinsic report quality.
( R )-Citramalate Synthase in Methanogenic Archaea (1999)

The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.
Activin A: Autocrine Regulator of Kidney Development and Repair (2008)

The research described in this review suggests a novel and important role for activin A in the developmental and repair processes of the kidney (Table 1). The results obtained in these studies indicate that activin A is a negative regulator of kidney development and plays an essential part in kidney diseases, such as acute renal failure or renal fibrosis. It is also possible that activin A is a key player in the pathophysiological processes of other kidney diseases, such as congenital urogenital abnormalities, renal cystic disease and renal cell carcinoma. Activin A is thus a potential target for therapeutic interventions in kidney diseases. To address this issue, more detailed analysis on the regulation of activin production, modulation of activin activity and activin target genes is required.
A fully automated object extraction system for the World Wide Web (2002)

This paper presents a fully automated object extraction system Omini. A distinct feature of Omini is the suite of algorithms and the automatically learned information extraction rules for discovering and extracting objects from dynamic Web pages or static Web pages that contain multiple object instances. We evaluated the system using more than 2,000 Web pages over 40 sites. It achieves 100% precision (returns only correct objects) and excellent recall (between 99% and 98%, with very few significant objects left out). The object boundary identification algorithms are fast, about 0.1 second per page with a simple optimization.
Concerning the Phosphorylation of Vicinal Diols (1988)

Abstract The specific use of n-butyllithium as the base for reaction of diethyl phosphorochloridate with diols leads to the formation of diphosphate esters in good to excellent yields without any evidence for the formation of 5-, 6-, or 7-membered cyclic phosphates.
Syntheses and Diels-Alder cycloaddition reactions of 4H-furo[3,4-b]indoles. A regiospecific Diels-Alder synthesis of ellipticine (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSyntheses and Diels-Alder cycloaddition reactions of 4H-furo[3,4-b]indoles. A regiospecific Diels-Alder synthesis of ellipticineGordon W. Gribble, Daniel J. Keavy, Deborah A. Davis, Mark G. Saulnier, Benjamin Pelcman, Timothy C. Barden, Mukund P. Sibi, Erik R. Olson, and Joseph J. BelBrunoCite this: J. Org. Chem. 1992, 57, 22, 5878–5891Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/jo00048a021https://doi.org/10.1021/jo00048a021research-articleACS PublicationsRequest reuse permissionsArticle Views1403Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Compounds in Milk Accompanying Feeding of Ethoxyquin (1968)

A feeding trial was conducted to determine if ethoxyquin, or its residues, is transferred from feed to milk when added to feed at 0.015% of the dry matter intake. A very low concentration (less than 7 t~g/ liter) of ethoxyquin was detected in milk by a fluorimetric procedure and by thin layer chromatography. Feeding ethoxyquin did not increase milk tocopherol, but was accompanied by appearance in milk of a compound (not yet identified) that interfered in the determination of ethoxyquin by a fluorimetric method, and in the determination of tocopherol by a method used before in our laboratory.
High-Mobility Group Box 1: A Novel Target for Treatment of Pseudomonas aeruginosa Keratitis (2015)

High-mobility group box 1 (HMGB1), a prototypic alarmin, mediates the systemic inflammatory response syndrome. Treatment with vasoactive intestinal peptide, an anti-inflammatory neuropeptide, downregulates proinflammatory cytokines and promotes healing in a susceptible (cornea perforates) model of Pseudomonas aeruginosa keratitis, and also significantly downregulates HMGB1 expression. Therefore, we examined targeting HMGB1 for the treatment of P. aeruginosa keratitis to avoid delivery and other issues associated with vasoactive intestinal peptide. For this, HMGB1 was silenced using small interfering RNA, whereas controls were treated with a nonspecific scrambled sequence small interfering RNA. Less disease was seen postinfection in siHMGB1 compared with control mice and was documented by clinical score and photographs with a slit lamp. Real-time RT-PCR and ELISA confirmed HMGB1 knockdown. RT-PCR analysis also revealed reduced mRNA levels of IL-1β, MIP-2, TNF-α, TLR4, and receptor for advanced glycation end products, whereas mRNA levels of anti-inflammatory TLRs single Ig IL-1-related receptor and ST2 were increased significantly. HMGB1 knockdown also decreased IL-1β and MIP-2 proteins, reducing polymorphonuclear cell number in the infected cornea. mRNA and protein levels of CXCL12 and CXCR4, as well as mononuclear cells, were reduced significantly after HMGB1 knockdown. Ab neutralization of HMGB1, infection with a clinical isolate, and recombinant HMGB1 treatment of resistant mice supported the silencing studies. These data provide evidence that silencing HMGB1 promotes better resolution of P. aeruginosa keratitis by decreasing levels of proinflammatory mediators (decreasing polymorphonuclear cell infiltration), increasing anti-inflammatory TLRs, reducing CXCL12 (preventing HMGB1/CXCL12 heterodimer formation), and signaling through CXCR4, reducing monocyte/macrophage infiltration.
Receptor-targeted recombinant adenovirus conglomerates: a novel molecular conjugate vector with improved expression characteristics (1997)

To develop improved strategies for gene transfer to hematopoietic cells, we have explored targeted gene transfer using molecular conjugate vectors (MCVs). MCVs are constructed by condensing plasmid DNA containing the gene of interest with polylysine (PL), PL linked to a replication-incompetent adenovirus (endosomolytic agent), and PL linked to streptavidin for targeting with biotinylated ligands. In this report, we compare gene transfer to K562 cells by using the previously described transferrin-targeted MCV (Trans-MCV) to a novel transferrin-targeted MCV. In the novel MCV, the transferred gene (luciferase) is in the genome of recombinant replication-incompetent adenovirus (recMCV), which also acts as the endosomolytic agent. The level of luciferase gene expression was fivefold higher in K562 cells transfected with Trans-recMCV than in cells transfected with Trans-MCV. Furthermore, targeted transfection with recMCV resulted in prolonged luciferase expression that declined 14 to 20 days after transfection, in comparison with Trans-MCV, where luciferase expression declined by 4 to 8 days. Moreover, targeted transfection of K562 cells with the Trans-recMCV resulted in persistent luciferase gene expression for 6 months. Analysis of luciferase gene expression in K562 single-cell clones that were subcloned 5 weeks after transfection with Trans-recMCV showed that 35 to 50% of the single-cell clones had intermediate to high levels of luciferase gene expression that was stable for 6 months, with the remaining clones showing low or no luciferase gene expression. Stable gene expression was associated with integration of adenovirus sequences into genomic DNA.