gebhart/patrepeval huggingface dataset:
Flow cytometric analysis of CD123 is useful for immunophenotyping classical Hodgkin lymphoma (2010)

Although the diagnosis of classical Hodgkin lymphoma (CHL) is traditionally made morphologically in tissue sections, it is now possible to diagnose CHL by flow cytometry (FC). In an effort to identify additional antigens on Hodgkin and Reed-Sternberg (HRS) cells that might be useful for immunophenotyping this lymphoma by FC, we examined the expression of CD123 (α chain of the IL-3R) on HRS cells and compared this with the expression of CD123 in other lymph node samples (372 tissue specimens, including 98 reactive and 274 neoplastic cases), using a nine-color FC reagent combination including anti-CD123. CD123 was found to be expressed on the majority of HRS cell populations (59% of 59 CHL lymph nodes examined), rarely on B-cell non-Hodgkin lymphomas (NHLs) (3 of 3 hairy cell leukemia cases, 3 of 29 chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) cases, 1 of 55 large B-cell lymphoma cases, but not on 95 other B-cell NHL examined), and in none of the 16 T-cell NHL cases examined. In reactive lymph nodes, CD123 expression was largely limited to plasmacytoid dendritic cells and rare histiocyte populations that can readily be distinguished from HRS populations. As the expression of this antigen is shown to be relatively limited in B- and T-NHL and in reactive lymph node populations, assessment of CD123 expression is useful for supporting the FC diagnosis of CHL.
Improvement of the dielectric properties of Ta2O5 through substitution with Al2O3 (1997)

For bulk ceramic materials, small substitutions of Al2O3 in Ta2O5 are shown to cause a moderate enhancement of the dielectric constant. For compositions near 0.95Ta2O5–0.05Al2O3, the temperature coefficient of the dielectric constant at 20 °C dramatically decreases, from more than 200 ppm/°C for pure Ta2O5 to less than 20 ppm/°C. The quality factors (Q) at 1 MHz, are 2000–5000. Measurements to 14 GHz at 20 °C show that the dielectric constant is maintained to microwave frequencies, with a Q of ∼600 at 5 GHz.
Respiratory system : a compilation of paintings depicting anatomy and embryology, physiology, pathology, pathophysiology, and clinical features and treatment of diseases (1979)

The most critically acclaimed of all of Dr. Frank H. Netter's works, this fully illustrated single book from the 8-volume/13-book reference collection includes: hundreds of world-renowned illustrations by Frank H. Netter, MD; informative text by recognized medical experts; anatomy, physiology, and pathology; and diagnostic and surgical procedures.
Ethanol, oxidative stress, and cytokine-induced liver cell injury (2002)

Both clinical findings and results of experiments with animal models of alcoholic hepatitis have shown the importance of cytokine-mediated cell-cell interactions in the onset of ethanol-induced liver damage. Proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta (IL-1 beta), and interleukin-6, are released from Kupffer cells or infiltrating neutrophils and macrophages and elicit defensive responses in parenchymal cells, including activation of apoptosis. Reactive oxygen species (ROS) and reactive nitrogen species (RNS), generated in response to cytokine-induced stress signals in parenchymal cells and also by activation of Kupffer cells and inflammatory cells, further mobilize cellular defense mechanisms. When these defensive responses are overwhelmed cells may die by necrosis, further stimulating inflammatory responses and infiltration of neutrophils. Chronic ethanol intake (i.e., many years of heavy alcohol use in human patients, several weeks or months in experimental animals) enhances the damaging consequences of these events through a variety of mechanisms. The formation of cytokines in the liver is stimulated by increasing circulating levels of endotoxin and by enhancing the responsiveness of Kupffer cells to such stimuli. In addition, ethanol promotes oxidative stress, both by increased formation of ROS and by depletion of oxidative defenses in the cell. Furthermore, liver cells from ethanol-treated animals are more susceptible to the cytotoxic effects of TNF-alpha and other cytokines than cells from control animals. Mitochondria play a critical role in the apoptotic response, and alterations in mitochondrial function after chronic ethanol treatment may contribute to enhanced cell death by apoptosis or necrosis. How the shift in the balance of cytokine-induced defensive and damage responses in hepatocytes contributes to the liver injury that occurs in alcoholic hepatitis remains poorly characterized and should be a rewarding area for future studies.
Developments in Nonsteroidal Antiandrogens Targeting the Androgen Receptor (2010)

Enemy of the (pro)state! The androgen receptor (AR) is closely involved in the formation and growth of prostate cancer. Currently, targeting the AR is a hot research area in the design and development of drugs against prostate cancer. Herein, nonsteroidal antiandrogen agents that target the AR are reviewed.
Transgenic mice expressing the tumor marker germ cell alkaline phosphatase: an in vivo tumor model for human cancer antigens. (1993)

We have generated a series of transgenic mouse lines harboring the entire human germ cell alkaline phosphatase (GCAP) gene linked to progressively longer sequences of flanking DNA. A 450-bp promoter sequence directs the expression of GCAP to the intestine and endothelial cells, while a 5' sequence of 1.7 kb directs GCAP expression to the spermatogenic lineage and to the eight-cell through the blastocyst stage of preimplantation development. The expression of GCAP in these FVB/N transgenic mice induces a cellular immune tolerance to GCAP. When mouse fibrosarcoma MO4 cells (C3H derived), stably transfected with the cloned GCAP gene, were injected s.c. in nontransgenic control (C3H x FVB/N) hybrid mice, GCAP-positive tumor cells were rejected. However, when GCAP-expressing transgenic (C3H x FVB/N) hybrid mice were challenged with these cells, GCAP-positive tumors developed. Tumors also developed in the transgenic hybrid mice upon injection of MO4 cells transfected with the highly homologous placental alkaline phosphatase (PLAP) cDNA in spite of the presence in PLAP of 10 amino acids that are different from the corresponding residues in GCAP. These GCAP transgenic mice will allow the study of the immune response associated with the repeated administration of conjugated or derivatized anti-GCAP and anti-PLAP monoclonal antibodies. They will also enable evaluation of the therapeutic potential of bifunctional antibodies for T-cell recruitment and destruction of GCAP/PLAP-producing tumor cells.
Optimizing a qPCR Gene Expression Quantification Assay for S. epidermidis Biofilms: A Comparison between Commercial Kits and a Customized Protocol (2012)

Staphylococcus epidermidis biofilm-related infections are a current concern within the medical community due to their high incidence and prevalence, particularly in patients with indwelling medical devices. Biofilm gene expression analysis by quantitative real-time PCR (qPCR) has been increasingly used to understand the role of biofilm formation in the pathogenesis of S. epidermidis infections. However, depending on the RNA extraction procedure, and cDNA synthesis and qPCR master mixes used, gene expression quantification can be suboptimal. We recently showed that some RNA extraction kits are not suitable for S. epidermidis biofilms, due to sample composition, in particular the presence of the extracellular matrix. In this work, we describe a custom RNA extraction assay followed by the evaluation of gene expression using different commercial reverse transcriptase kits and qPCR master mixes. Our custom RNA extraction assay was able to produce good quality RNA with reproducible gene expression quantification, reducing the time and the costs associated. We also tested the effect of reducing cDNA and qPCR reaction volumes and, in most of the cases tested, no significant differences were found. Finally, we titered the SYBR Green I concentrations in standard PCR master mixes and compared the normalized expression of the genes icaA, bhp, aap, psmβ1 and agrB using 4 distinct biofilm forming S. epidermidis strains to the results obtained with commercially available kits. The overall results demonstrated that despite some statistically, but not biologically significant differences observed, the customized qPCR protocol resulted in the same gene expression trend presented by the commercially available kits used.
A novel clustered dielectric cubes metamaterial (CDC-MTM) (2006)

We investigate here a nonmetallic metamaterial (MTM) made of clustered dielectric cubes, where left-handed (LH) bands are generated by antisymmetric modes producing plasmonic artificial magnetic dipoles alternating with electric dipoles, so as to generate negative refractive index (NRI) characteristics. This structure bears some similarity with the metallic nanopillar pairs made of gold introduced in A.N. Grigorenko (2005) for magnetic activity at visible frequencies
Integrins and other cell adhesion molecules (1990)

Cell-cell and cell-substratum interactions are mediated through several different families of receptors. In addition to targeting cell adhesion to specific extracellular matrix proteins and ligands on adjacent cells, these receptors influence many diverse processes including cellular growth, differentiation, junction formation, and polarity. Several families of adhesion receptors have been identified. These include: 1) the integrins, heterodimeric molecules that function both as cell-substratum and cell-cell adhesion receptors; 2) the adhesion molecules of the immunoglobulin superfamily, which are involved in cell-cell adhesion and especially important during embryo-genesis, wound healing, and the inflammatory response; 3) the cadherins, developmentally regulated, calcium-dependent homophilic cell-cell adhesion proteins; 4) the LEC-CAMs, cell adhesion molecules with lectin-like domains that mediate white blood cell/endothelial cell adhesion; and 5) homing receptors that target lymphocytes to specific lymphoid tissue. In this review we summarize recent data describing the structure and function of some of these cell adhesion molecules (with special emphasis on the integrin family) and discuss the possible role of these molecules in development, inflammation, wound healing, coagulation, and tumor metastasis.
MONOCLONAL ANTIBODIES TO MOUSE MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS (1982)

As part of our continuing effort to produce a library of hybridoma antibodies specific for the products of the mouse major histocompatibility complex (MHC), nine antibodies reacting with antigens of the H-2d haplotype have been produced by cell fusion between immune spleen cells and the SP2/0.Ag.14 cell, of H-2d origin. Serological characterization revealed that seven antibodies reacted with H-2 antigens and two with Ia antigens. Of the anti-H-2 antibodies, four detected private specificities of H-2Kd or H-2Dd antigens and three detected public specificities of H-2d and other haplotypes. One of the anti-Ia antibodies detected a private Ia specificity corresponding to Ia.23 and the other detected a previously undescribed public specificity. Anti-H-2d hybridoma cells represent a potential "autoreactive" situation in that the antibodies produced by the cells should react with their own H-2 antigens unless expression of the corresponding H-2d antigens in these cells was altered. In order to examine whether H-2d antigens continued to be expressed on these anti-H-2d hybridoma cells, binding of 125I-labeled monoclonal anti-H-2Kd and/or H-2Dd antibodies was studied. Among the four hybridoma clones tested, three bound specifically three independent 125I-labeled anti-H-2d antibodies, including two cases in which binding of autologous antibodies was detected. The last clone did not bind any of the anti-H-2d antibodies, although it bound an anti-H-2k antibody, indicating selective loss of H-2d antigens. These observations demonstrate that neither loss nor retention of H-2d antigen expression on the cell surface is obligatory in hybridoma cells producing anti-H-2d antibodies.
Neuronal nicotinic acetylcholine receptor β‐subunit is coded for by the cDNA clone α4 (1987)

Acetylcholine receptors (AChRs) with high affinity for nicotine but no affinity for alpha-bungarotoxin, which have been purified from rat and chicken brains by immuno-affinity chromatography, consist of two types of subunits, alpha and beta. The beta-subunits form the ACh binding sites. Putative nicotinic AChR subunit cDNAs alpha 3 and alpha 4 have been identified by screening cDNA libraries prepared from rat PC12 cells and rat brain with cDNA probes encoding the mouse muscle AChR alpha-subunit. Here we determine the amino-terminal amino acid sequence of the rat brain AChR beta-subunit by protein microsequencing to be the same as amino acid residues 27-43 of the protein which could be coded by alpha 4. Further, we present evidence consistent with a subunit stoichiometry of alpha 3 beta 2 for this neuronal nicotinic AChR.
Somatic chromosomal engineering identifies BCAN-NTRK1 as a potent glioma driver and therapeutic target (2017)

The widespread application of high-throughput sequencing methods is resulting in the identification of a rapidly growing number of novel gene fusions caused by tumour-specific chromosomal rearrangements, whose oncogenic potential remains unknown. Here we describe a strategy that builds upon recent advances in genome editing and combines ex vivo and in vivo chromosomal engineering to rapidly and effectively interrogate the oncogenic potential of genomic rearrangements identified in human brain cancers. We show that one such rearrangement, an microdeletion resulting in a fusion between Brevican (BCAN) and Neurotrophic Receptor Tyrosine Kinase 1 (NTRK1), is a potent oncogenic driver of high-grade gliomas and confers sensitivity to the experimental TRK inhibitor entrectinib. This work demonstrates that BCAN-NTRK1 is a bona fide human glioma driver and describes a general strategy to define the oncogenic potential of novel glioma-associated genomic rearrangements and to generate accurate preclinical models of this lethal human cancer.
Carrier‐bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen‐induced basophil activation in allergic patients (2012)

Carrier-bound Der p 2 peptides should allow avoiding IgE-mediated side-effects, and because of their low potential to activate allergen-specific T cells, they may reduce late-phase side-effects during SIT. Further, these peptides may be also useful for prophylactic vaccination.
Molecular immunology lessons from therapeutic T‐cell receptor gene transfer (2010)

The T-cell receptor (TCR) is critical for T-cell lineage selection, antigen specificity, effector function and survival. Recently, TCR gene transfer has been developed as a reliable method to generate ex vivo large numbers of T cells of a given antigen-specificity and functional avidity. Such approaches have major applications for the adoptive cellular therapy of viral infectious diseases, virus-associated malignancies and cancer. TCR gene transfer utilizes retroviral or lentiviral constructs containing the gene sequences of the TCR-alpha and TCR-beta chains, which have been cloned from a clonal T-cell population of the desired antigen specificity. The TCR-encoding vector is then used to infect (transduce) primary T cells in vitro. To generate a transduced T cell with the desired functional specificity, the introduced TCR-alpha and TCR-beta chains must form a heterodimer and associate with the CD3 complex in order to be stably expressed at the T-cell surface. In order to optimize the function of TCR-transduced T cells, researchers in the field of TCR gene transfer have exploited many aspects of basic research in T-cell immunology relating to TCR structure, TCR-CD3 assembly, cell-surface TCR expression, TCR-peptide/major histocompatibility complex (MHC) affinity and TCR signalling. However, improving the introduction of exogenous TCRs into naturally occurring T cells has provided further insights into basic T-cell immunology. The aim of this review was to discuss the molecular immunology lessons learnt through therapeutic TCR transfer.
Induction of immune responses in vivo with small numbers of veiled (dendritic) cells. (1983)

The role of dendritic or veiled cells (VC) from lymph nodes or spleens of rats and mice in initiating immune responses in vivo has been investigated. Host-versus-graft responses were induced by injection of VC from spleens of (C57BL/10 X CBA) F1 mice into the footpads of parental strain (CBA) animals and measured by the increase in the weight of the draining popliteal lymph nodes. The potency of VC to induce the responses was 100-fold greater than that of unseparated spleen cells. The in vivo effect of VC was not limited to this direct allogeneic stimulation because autologous VC could also be used in the induction of an experimental autoimmune disease. In these studies, experimental allergic encephalomyelitis was produced in Lewis rats by injection of guinea pig brain and spinal cord material emulsified in Freund's complete adjuvant. Small numbers of VC from spleens or lymph nodes of rats showing clinical signs of experimental allergic encephalomyelitis induced a mild form of the disease when injected intravenously into normal Lewis rats. Thus, VC carrying antigen, either as an integral part of their surface membrane or acquired during exposure to antigenic substances, appeared to be very potent agents for the induction of immune responses.
Third Party Donor Derived EBV Specific T Cells for the Treatment of Refractory EBV-Related Post-Transplant Lymphomas (2014)

Adoptive immunotherapy is an effective strategy for the treatment of EBV+ lymphoproliferative diseases (EBV-LPD) arising after allogeneic hematopoietic stem cell (HSCT) or solid organ transplant (SOT). This approach is often limited by an inability to generate donor derived in vitro expanded EBV-specific cytotoxic T-lymphocyte (EBV-CTL) lines in a timely manner and/or donor derived EBV CTLs being restricted by non-shared HLA alleles in the patient. We have treated 28 consecutive patients for EBV LPD with in vitro expanded EBV-CTLs derived from a donor other than the patient or their transplant (HSCT or SOT) donor. EBV CTLs were selected from a bank of 345 lines generated under GMP conditions from normal donors. Patients were recipients of unmodified (n=7), T cell depleted (n=7) or unrelated cord blood (n=7) HSCT or one (n=5) or two (n=2) SOTs. Patients received infusions of 3rd party EBV-CTLs after failing a median of 2 prior therapies including rituximab in all but one case. Three patients failed prior infusions with EBV-CTLs which were autologous (n=1) or derived from their original HSCT donor (n=2). In these two patients it was demonstrated that the donor derived EBV CTLs were restricted by a non-shared HLA allele. Third party EBV-CTLs were selected on the basis of HLA matching at a minimum of 2/8 recipient alleles. When known, EBV-CTLs restricted through HLA alleles present on the EBV+ tumor were selected. A total of 25 lines were infused. We evaluated HLA restriction in 20 of these lines and inferred it in five. CTLs were restricted by a single HLA allele (n=12), by two alleles (n=6) and less frequently by > than two alleles (n=2). Patients received a median of 6 infusions most at 1x106 EBV-CTL/kg. Five patients received EBV-CTLs from >1 3rd party donor. Infusions were associated with minimal toxicity. Sixteen patients achieved CR, two patients PR and two SD. Eight patients died of progressive disease, five shortly after the first infusion (17- 29 days). Response to EBV CTL therapy correlated with HLA restriction but not the degree of HLA matching. Radiographic and clinical responses correlated with detectable increases in the frequency of CTL precursors in the blood. However durable EBV CTL engraftment was not seen. This study demonstrates a high response rate among patients with otherwise refractory EBV malignancy treated with EBV specific 3rd party CTLs. EBV CTLs can be effective when selected based on restriction to shared alleles despite significant HLA disparity. The bank of EBV specific T cells can provide an immediate source of HLA partially matched appropriately restricted T cells for adoptive immunotherapy to treat EBV associated malignancy. This enables treatment early in the course of disease and is anticipated to maximize the response rate.
Feedback techniques for continuity and synchronization in multimedia information retrieval (1995)

Future advances in storage and networking technologies will make it feasible to build multimedia on-demand information servers capable of providing services similar to those of a neighborhood videotape rental store over metropolitan area networks. Such multimedia information servers must not only support retrieval of continuous media units (such as video frames and audio samples), but also preserve synchrony among playback of the different media components constituting a multimedia object. We develop techniques for supporting continuous and synchronous retrieval from multimedia servers. We present feedback techniques by which, during retrieval of multimedia objects from a multimedia server to mediaphones, the multimedia server uses lightweight messages called feedback units transmitted periodically back to it (by mediaphones) to detect impending discontinuities as well as asynchronies at mediaphones. The multimedia server then preventively readjusts media transmission so as to avoid either anomaly, and steers the mediaphones back to synchrony. Given the available buffer sizes at mediaphones and the maximum tolerable asynchrony, we present methods to determine the minimum rate at which feedback units must be transmitted so as to maintain both continuity and synchronization. These feedback techniques remain robust even in the presence of playback rate mismatches and network delay jitter, and their initial simulation for video-audio playback yields a feedback rate of one per 1,000 media units to keep the asynchrony within 250ms, showing that the overhead due to feedback transmission is very small. The constant rate feedback techniques developed in this article form the basis of a prototype on-demand information server being developed at the UCSD Multimedia Laboratory.
Signal transduction in Campylobacter jejuni-induced cytokine production (2005)

Campylobacter jejuni is the leading cause of food-borne illness in the USA and one of the most common causes of diarrhoea worldwide. Central to its pathogenicity is its ability to induce the production of proinflammatory cytokines such as interleukin (IL)-8 in intestinal epithelial cells. Here, we demonstrated that C. jejuni infection of intestinal epithelial cells results in the activation of the ERK and p38 mitogen-activated protein kinases and that the ERK kinase pathway is essential for IL-8 production. We found that MAP kinase stimulation leading to IL-8 secretion requires C. jejuni gene products whose production is stimulated upon contact with epithelial cells. We also found that C. jejuni flagellin is a very poor stimulator of Toll-like receptor (TLR)-5 and therefore does not play a significant role in the stimulation of cytokine production.