gebhart/patrepeval huggingface dataset:
Double-stranded RNA interferes with gene function at the single-cell level in cereals (2000)

Double-stranded RNA (dsRNA) has been shown to specifically interfere with gene function in several organisms including tobacco and the model plant Arabidopsis. Here, we report on rapid and sequence-specific interference of dsRNA with gene function in cereals. Delivery of cognate dsRNA into single epidermal cells of maize, barley or wheat by particle bombardment interfered with the function of co-bombarded UidA (GUS) and TaGLP2a:GFP reporter genes. Cognate dsRNA was also found to specifically interfere with the function of the endogenous genes A1 and Ant18 encoding dihydroflavonol-4-reductase in maize and barley, respectively. Dihydroflavonol-4-reductase is an essential enzyme of the anthocyanin biosynthetic pathway in maize and barley. This pathway can be induced by transient expression of the C1- and b-Peru genes that encode transcription factors. In the presence of dsRNA corresponding to the dihydroflavonol-4-reductase gene, C1- and b-Peru-dependent, cell-autonomous accumulation of red anthocyanin pigments in bombarded cells of maize and barley was reduced. dsRNA was also demonstrated to negatively interfere with Mlo, which encodes a negative regulator of race non-specific resistance to the powdery mildew fungus in barley. In the presence of Mlo dsRNA, transformed cells became more resistant, thereby phenocopying plants that carry a heritable loss-of function mlo resistance allele. The results suggest that direct delivery of dsRNA to cereals leads to a rapid and sequence-specific interference with gene function at the single-cell level.
Linear Pre-Coding Performance in Measured Very-Large MIMO Channels (2011)

Wireless communication using very-large multiple-input multiple-output (MIMO) antennas is a new research field, where base stations are equipped with a very large number of antennas as compared to previously considered systems. In theory, as the number of antennas increases, propagation properties that were random before start to become deterministic. Theoretical investigations with independent identically distributed (i.i.d.) complex Gaussian (Rayleigh fading) channels and unlimited number of antennas have been done, but in practice we need to know what benefits we can get from very large, but limited, number of antenna elements in realistic propagation environments. In this study we evaluate properties of measured residential-area channels, where the base station is equipped with 128 antenna ports. An important property to consider is the orthogonality between channels to different users, since this property tells us how advanced multi-user MIMO (MU-MIMO) pre-coding schemes we need in the downlink. We show that orthogonality improves with increasing number of antennas, but for two single-antenna users there is very little improvement beyond 20 antennas. We also evaluate sum-rate performance for two linear pre-coding schemes, zero-forcing (ZF) and minimum mean squared error (MMSE), as a function of the number of base station antennas. Already at 20 base station antennas these linear pre-coding schemes reach 98% of the optimal dirty-paper coding (DPC) capacity for the measured channels.
A 2.5-2.7 THz room temperature electronic source (2011)

We report on a room temperature 2.5 to 2.7 THz electronic source based on frequency multipliers. The source utilizes a cascade of three frequency multipliers with W-band power amplifiers driving the first stage multiplier. Multiple-chip multipliers are utilized for the two initial stages to improve the power handling capability and a sub-micron anode is utilized for the final stage tripler. Room temperature measurements indicate that the source can put out a peak power of about 14 microwatts with more than 4 microwatts in the 2.5 to 2.7 THz range.
Effects of two cannabinoids on hepatic microsomal cytochrome P-450. (1986)

The effects of cannabidiol (CBD) and delta 9-tetrahydrocannabinol (delta 9-THC) on the synthesis and degradation of hepatic microsomal cytochrome P-450 were studied in mice. Cannabinoids used (10, 50 and 100 mg/kg, i.p.) did not affect delta-aminolevulinic acid synthetase activity in the liver. delta 9-THC-treatment (10, 50 and 100 mg/kg, i.p.) markedly stimulated heme oxygenase activity in hepatic 18000 X g supernatant fractions in a dose-dependent manner, whereas CBD-treatment was without effect. In vitro experiments, CBD and delta 9-THC (40 to 160 microM) markedly inhibited nicotinamide adenine dinucleotide phosphate (NADPH)-induced lipid peroxidation in hepatic microsomes. When CBD was incubated with the hepatic microsomes in the presence of an NADPH-generating system, cytochrome P-450 content decreased significantly. However, delta 9-THC showed no effects in similar experiments. The rate of decrease in the cytochrome P-450 content using CBD (160 microM) was 0.212 nmol/mg protein/20 min in microsomes from control mice. This value increased significantly in microsomes from phenobarbital-treated mice (0.792 nmol/mg protein/20 min) but not in those from 3-methylcholanthrene-treated mice (0.190 nmol/mg protein/20 min). The metabolic rate (per nmol cytochrome P-450) of CBD was also increased significantly by phenobarbital-treatment but not by 3-methylcholanthrene-treatment. These results suggest that CBD metabolites rather than CBD itself, play some role in the decreasing effect on cytochrome P-450 content in the hepatic microsomes in vitro, and that the microsomal formation of reactive metabolite of CBD is increased by phenobarbital-treatment.
Feature based robot navigation: using fuzzy logic and interval analysis (2005)

This work describes a new approach for mobile robot navigation using interval analysis and fuzzy logic. The robot is equipped with inertial sensors, encoders and ultrasonic sensors. The map used for this study is two-dimensional and it is assumed to be known. Multiple sensor fusion for robot localisation and navigation has attracted a lot of interested in recent years. The most commonly used approach is based on Kalman filter and other stochastic filters. Here we propose an alternative approach using interval analysis with multiple sets of ultrasonic measurements. Interval analysis has been already successfully applied in the past for robot localisation. But the results obtained may be conservative. Therefore this approach is extended using multiple sets of ultrasonic measurements, which results in estimation of multiple interval robot positions. These multiple interval robot positions are then fused using fuzzy logic to give a less conservative interval robot position estimate. Also interval analysis based algorithm can be used only in the presence of land marks. This problem is overcome here using additional sensors such as encoders and inertial sensors, which gives an estimate of the robot position using fuzzy logic in the absence of land marks.
Metagenomic systems biology of the human gut microbiome reveals topological shifts associated with obesity and inflammatory bowel disease (2011)

The human microbiome plays a key role in a wide range of host-related processes and has a profound effect on human health. Comparative analyses of the human microbiome have revealed substantial variation in species and gene composition associated with a variety of disease states but may fall short of providing a comprehensive understanding of the impact of this variation on the community and on the host. Here, we introduce a metagenomic systems biology computational framework, integrating metagenomic data with an in silico systems-level analysis of metabolic networks. Focusing on the gut microbiome, we analyze fecal metagenomic data from 124 unrelated individuals, as well as six monozygotic twin pairs and their mothers, and generate community-level metabolic networks of the microbiome. Placing variations in gene abundance in the context of these networks, we identify both gene-level and network-level topological differences associated with obesity and inflammatory bowel disease (IBD). We show that genes associated with either of these host states tend to be located at the periphery of the metabolic network and are enriched for topologically derived metabolic "inputs." These findings may indicate that lean and obese microbiomes differ primarily in their interface with the host and in the way they interact with host metabolism. We further demonstrate that obese microbiomes are less modular, a hallmark of adaptation to low-diversity environments. We additionally link these topological variations to community species composition. The system-level approach presented here lays the foundation for a unique framework for studying the human microbiome, its organization, and its impact on human health.
Network Coding for Mobile Devices - Systematic Binary Random Rateless Codes (2009)

In this work we consider the implementation of random linear network coding (RLNC) on battery constrained mobile devices with low computational capabilities such as; sensors, mobile phones and personal digital assistants (PDAs). It is non-trivial to create an efficient implementation of RLNC which is needed to ensure high throughput, low computational requirements and energy consumption. As a consequence there does not, to the best of our knowledge, exist any such implementation for mobile device that allow for throughput close to what can be achieved in e.g. wireless local area network (WLAN). In this paper we propose to base RLNC on the binary Galois field and to use a systematic code. We have implemented this approach in C++ and Symbian C++ and achieve synthetic encoding/decoding throughput of up to 40/30 MB/s on a Nokia N95-8GB mobile phone and 1.5/1.0 GB/s on a high end laptop.
Alan Kay: Transforming the Computer into a Communication Medium (2007)

Alan Kay is referred to as the "father of the personal computer" because his 1969 doctoral thesis described an early prototype of personal computing. Kay's ideas contributed to the transformation of the computer from a calculating machine to a communication medium. This article focuses on Kay's vision for personal computing.
Stochastic properties of processive cytidine DNA deaminases AID and APOBEC3G (2008)

Activation-induced (cytidine) deaminase (AID) efficiently introduces multiple and diversified deaminations in immunoglobulin (Ig) variable and switch regions. Here, we review studies of AID, and the APOBEC family member, APOBEC3G, demonstrating that both enzymes introduce multiple deaminations by processive action on single-stranded DNA and that deaminations occur stochastically at hot- and cold-spot targets. In a more detailed analysis of AID, we examine phosphorylation-null mutants, particularly, S38A and S43P. S43P mutant AID has been identified in a patient with hyper-IgM immunodeficiency syndrome. The phosphorylation-null mutants have essentially the same specific activity, processivity and ability to undergo transcription-dependent deamination compared with wild-type (WT) AID. Although the phosphorylation-null mutants still deaminate 5'-WRC hot spots, the mutant deamination spectra differ from WT AID. The mutants strongly prefer two motifs, 5'AGC and 5'GGC, which are disfavoured by WT AID. Differences in deamination specificities can be attributed primarily to the replacement of Ser rather than to the absence of phosphorylation. The 5'GGC motif occurs with exceptionally high frequency on the non-transcribed strand of human switch regions, IgG4 and IgE. The potential for S43P to catalyse large numbers of aberrant deaminations in switch region sequences suggests a possible relationship between non-canonical AID deamination specificity and a loss of antibody diversification.
Is privacy now possible? A brief history of an obsession (2001)

L'epoque contemporaine semble etre traversee par une angoisse, celle de la disparition de la vie privee, qui semble correlative du caractere obsessionnel, dans le contexte de la societe americaine, de l'idee de vie privee. L'A. tente une genealogie de cette obsession de la vie privee, en relation avec les differents problemes sociaux qui ont occupe le devant de la vie publique americaine dans les annees 1960-70
Analog-to-Information Conversion via Random Demodulation (2006)

Many problems in radar and communication signal processing involve radio frequency (RF) signals of very high bandwidth. This presents a serious challenge to systems that might attempt to use a high-rate analog-to-digital converter (ADC) to sample these signals, as prescribed by the Shannon/Nyquist sampling theorem. In these situations, however, the information level of the signal is often far lower than the actual bandwidth, which prompts the question of whether more efficient schemes can be developed for measuring such signals. In this paper we propose a system that uses modulation, filtering, and sampling to produce a low-rate set of digital measurements. Our "analog-to-information converter" (AIC) is inspired by the theory of compressive sensing (CS), which states that a discrete signal having a sparse representation in some dictionary can be recovered from a small number of linear projections of that signal. We generalize the CS theory to continuous-time sparse signals, explain our proposed AIC system in the CS context, and discuss practical issues regarding implementation
A de novo loss-of-function GRIN2A mutation associated with childhood focal epilepsy and acquired epileptic aphasia (2017)

We identified a de novo missense mutation in the GRIN2A gene in a patient with childhood focal epilepsy and acquired epileptic aphasia. The mutant decreases NMDAR activation suggesting NMDAR hypofunction may contribute to the epilepsy pathogenesis.
Mesoporous silica-coated luminescent Eu3+doped GdVO4nanoparticles for multimodal imaging and drug delivery (2014)

We describe a simple method for synthesizing mesoporous silica-coated luminescent europium-doped gadolinium vanadate (GdVO4:Eu3+@mSiO2) nanoparticles. Their biomedical applications as a potential imaging nanoprobe for both fluorescence imaging and magnetic resonance imaging (MRI) and as a simultaneous anti-cancer drug delivery vehicle are also discussed. Eu3+ doped GdVO4 nanoparticles exhibit strong red photoluminescence and the Gd3+ in GdVO4 can be used as a T1 contrast agent for MRI. T1-weighted MR contrast enhancement as well as sustained intracellular drug delivery can be achieved by the mesoporous silica layer coating onto a single nanoparticle. Enhanced T1 MR contrast was proven by relaxometric studies on water access to the paramagnetic core. GdVO4:Eu3+@mSiO2 nanoparticles as a new type of theragnostic (imaging and treatment) agent can provide new opportunities in a cancer treatment.
6-Hydroxydopamine-induced Apoptosis Is Mediated via Extracellular Auto-oxidation and Caspase 3-dependent Activation of Protein Kinase Cδ (2005)

6-Hydroxydopamine is a neurotoxin commonly used to lesion dopaminergic pathways and generate experimental models for Parkinson disease, however, the cellular mechanism of 6-hydroxydopamine-induced neurodegeneration is not well defined. In this study we have explored how 6-hydroxydopamine neurotoxicity is initiated. We have also investigated downstream signaling pathways activated in response to 6-hydroxydopamine, using a neuronal-like, catecholaminergic cell line (PC12 cells) as an in vitro model system. We have shown that 6-hydroxydopamine neurotoxicity is initiated via extracellular auto-oxidation and the induction of oxidative stress from the oxidative products generated. Neurotoxicity is completely attenuated by preincubation with catalase, suggesting that hydrogen peroxide, at least in part, evokes neuronal cell death in this model. 6-Hydroxydopamine does not initiate toxicity by dopamine transporter-mediated uptake into PC12 cells, because both GBR-12909 and nisoxetine (inhibitors of dopamine and noradrenaline transporters, respectively) failed to reduce toxicity. 6-Hydroxydopamine has previously been shown to induce both apoptotic and necrotic cell-death mechanisms. In this study oxidative stress initiated by 6-hydroxydopamine caused mitochondrial dysfunction, activation of caspases 3/7, nuclear fragmentation, and apoptosis. We have shown that, in this model, proteolytic activation of the proapoptotic protein kinase Cdelta (PKCdelta) is a key mediator of 6-hydroxydopamine-induced cell death. 6-Hydroxydopamine induces caspase 3-dependent cleavage of full-length PKCdelta (79 kDa) to yield a catalytic fragment (41 kDa). Inhibition of PKCdelta (with rottlerin or via RNA interference-mediated gene suppression) ameliorates the neurotoxicity evoked by 6-hydroxydopamine, implicating this kinase in 6-hydroxydopamine-induced neurotoxicity and Parkinsonian neurodegeneration.
Interferon Gamma-1b Compared with Placebo in Metastatic Renal-Cell Carcinoma (1998)

No difference in outcome was observed in patients with metastatic renal-cell carcinoma who were treated with interferon gamma-1b as compared with placebo. These results emphasize the necessity of testing the efficacy of immunomodulators in randomized studies.
Genomic transcriptional profiling identifies a candidate blood biomarker signature for the diagnosis of septicemic melioidosis (2009)

Blood transcriptional patterns distinguish patients with septicemic melioidosis from patients with sepsis caused by other pathogens. Once confirmed in a large scale trial this diagnostic signature might constitute the basis of a differential diagnostic assay.
Phylogeny of rice genomes with emphasis on origins of allotetraploid species (1999)

The rice genus, Oryza, which comprises 23 species and 9 recognized genome types, represents an enormous gene pool for genetic improvement of rice cultivars. Clarification of phylogenetic relationships of rice genomes is critical for effective utilization of the wild rice germ plasm. By generating and comparing two nuclear gene (Adh1 and Adh2) trees and a chloroplast gene (matK) tree of all rice species, phylogenetic relationships among the rice genomes were inferred. Origins of the allotetraploid species, which constitute more than one-third of rice species diversity, were reconstructed based on the Adh gene phylogenies. Genome types of the maternal parents of allotetraploid species were determined based on the matK gene tree. The phylogenetic reconstruction largely supports the previous recognition of rice genomes. It further revealed that the EE genome species is most closely related to the DD genome progenitor that gave rise to the CCDD genome. Three species of the CCDD genome may have originated through a single hybridization event, and their maternal parent had the CC genome. The BBCC genome species had different origins, and their maternal parents had either a BB or CC genome. An additional genome type, HHKK, was recognized for Oryza schlechteri and Porteresia coarctata, suggesting that P. coarctata is an Oryza species. The AA genome lineage, which contains cultivated rice, is a recently diverged and rapidly radiated lineage within the rice genus.
GnRH-Induced Ca 2+ Oscillations and Rhythmic Hyperpolarizations of Pituitary Gonadotropes (1992)

Secretion of gonadotropic hormones from pituitary gonadotropes in response to gonadotropin-releasing hormone (GnRH) is essential for regulation of reproductive potential. Gonadotropes from male rats exhibited an unusual form of cellular excitation that resulted from periodic membrane hyperpolarization. GnRH induced an oscillatory release of intracellular Ca2+ via a guanosine triphosphate (GTP) binding protein-coupled phosphoinositide pathway and hyperpolarized the gonadotrope periodically by opening apamin-sensitive Ca(2+)-activated K+ (SK) channels. Each hyperpolarization was terminated by firing of a few action potentials that may result from removal of inactivation from voltage-gated Na+ and Ca2+ channels.
Plerixafor-moblized stem cells alone are capable of inducing early engraftment across the MHC-haploidentical canine barrier (2010)

To the editor:

Donahue and colleagues published in Blood a study comparing CD34+ cells mobilized by plerixafor (AMD3100) or granulocyte colony-stimulating factor (G-CSF) in macaques.[1][1] Different gene profiling patterns and cell types were produced based on mobilization protocol. The authors
Cytotoxicity and Microbicidal Activity of Electrolyzed Strong Acid Water and Acidic Hypochlorite Solution under Isotonic Conditions (1999)

The cytotoxic effects of electrolyzed strong acid water and acidic hypochlorite solution, as well as these solutions after isotonization, against cultivated L cells were compared along with their microbicidal activities. Isotonization was accompanied by a reduction in the cytotoxic effects of these solutions against L cells. Microbicidal activity was also reduced somewhat but was still retained after isotonization. No difference was observed in these properties between these antiseptic solutions. The results obtained indicate that acidic hypochlorite solution may be useful as well as acidic electrolyzed water.
Bend-resistant fundamental mode operation in ytterbium-doped leakage channel fibers with effective areas up to 3160 µm2 (2006)

We report fundamental mode operation in ytterbium-doped leakage channel fibers with effective areas up to 3160 microm(2). These fibers are bend-resistant. Lasers with slope efficiency ~60% and M(2) ~1.3 has been demonstrated. Bend loss and amplifier performance have also been characterized.
Effects of Cholecystokinin (CCK)-4, Nonsulfated CCK-8, and Sulfated CCK-8 on Pancreatic Somatostatin, Insulin, and Glucagon Secretion in the Dog: Studiesin Vitro* (1984)

The effect of cholecystokinin (CCK)-4, nonsulfated CCK-8 (CCK-8), and sulfated CCK-8 (CCK-8-S) on endocrine pancreas function was investigated in the isolated perfused dog pancreas in the presence of 5.5 mM glucose. CCK-4 and CCK-8 at concentrations of 1, 10, and 100 nM dose dependently stimulated pancreatic SRIF, insulin, and glucagon release. The insulinotropic and glucagonotropic potency of CCK-8 was significantly greater than that of CCK-4, whereas the effect on SRIF secretion was similar. Furthermore, CCK-8-S and CCK-8 at concentrations of 0.1, 1, and 10 nM caused a dose-dependent increase in pancreatic A, B, and D cell secretion. The CCK-8-S was a more potent insulinotropic agent than CCK-8. It is suggested that these principal molecular CCK forms qualify for a physiological modulatory role in the endocrine pancreas.
Preimplantation genetic screening reveals a high incidence of aneuploidy and mosaicism in embryos from young women undergoing IVF (2005)

The rates of mosaicism and aneuploidy in these embryos from young IVF patients are similar to those published for older women. We found the best confirmation rate after a diagnosis based on two cells, where both blastomeres showed the same chromosomal abnormality. In contrast, after a mosaic diagnosis the confirmation rate was low. The present study provides the first detailed reanalysis data of embryos analysed by PGS and clearly demonstrates the impact of mosaicism on the reliability of the PGS diagnosis.
[Prognostic value of oxygen metabolism indices in the diffuse purulent peritonitis complicated by sepsis]. (2000)

There were the results of examination of 42 patients with diffuse purulent peritonitis, complicated by sepsis, analyzed. High risk of the oxygen debt, occurring due to limitation of the oxygen delivery to tissues, was noted. Normalization of delivery consumption ratio of oxygen and elimination of the oxygen debt in the early course of the disease are needed for the disease severity reduction and the mortality lowering.