gebhart/patrepeval huggingface dataset:
Preparation and Dissolution Characteristics of Prolonged Release Mebeverine-HCL Beads (1994)

Different batches of slow release mebeverine-HCl beads were prepared by pan coating technique using different release retarding polymers viz Eudragit RL100, Eudragit RS100 and Ethyl cellulose. The thickness of the coats was controlled by changing the amounts of the added polymers. Pre- and overcoating of the beads with bees wax was also carried out. Mixtures of pre-waxed Eudragit RS100 coated and uncoated beads in different ratios were prepared to control both drug content and release.Dissolution profiles of mebeverine HCl from the prepared beads were investigated using USP XX rotating basket method. Prolonged release of mebeverine-HCl was obtained from different batches of the coated beads with the advantage of no initial dumping of the water soluble drug. The release of mebeverine-HCl from the beads coated with acrylic resins and ethyl cellulose as well as waxed acrylic resins coated beads was diffusion controlled according to Higuchi model. Beads coated with ethyl cellulose showed a different release pattern when pre-or overcoated with wax. By altering the ratios of prewaxed Eudragit Rs100 coated and uncoated beads in formulated mixtures, it was possible to control both mebeverine-HCl content and release rate.
Monolithic integration of rare-earth oxides and semiconductors for on-silicon technology (2014)

Several concepts of integration of the epitaxial rare-earth oxides into the emerging advanced semiconductor on silicon technology are presented. Germanium grows epitaxially on gadolinium oxide despite lattice mismatch of more than 4%. Additionally, polymorphism of some of the rare-earth oxides allows engineering of their crystal structure from hexagonal to cubic and formation of buffer layers that can be used for growth of germanium on a lattice matched oxide layer. Molecular beam epitaxy and metal organic chemical vapor deposition of gallium nitride on the rare-earth oxide buffer layers on silicon is discussed.
Computer-Assisted Scanning of Ligand Interactions:  Analysis of the Fructose 1,6-Bisphosphatase−AMP Complex Using Free Energy Calculations (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTComputer-Assisted Scanning of Ligand Interactions: Analysis of the Fructose 1,6-Bisphosphatase−AMP Complex Using Free Energy CalculationsMark D. Erion, Paul D. van Poelje, and M. Rami ReddyView Author Information Metabasis Therapeutics, Inc., 9390 Towne Centre Drive San Diego, California 92121 Cite this: J. Am. Chem. Soc. 2000, 122, 25, 6114–6115Publication Date (Web):June 8, 2000Publication History Received23 February 2000Revised10 May 2000Published online8 June 2000Published inissue 1 June 2000https://doi.org/10.1021/ja000651vCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views176Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (49 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Free energy,Genetics,Ligands,Noncovalent interactions,Screening assays Get e-Alerts
Production of Anti-Fibroblast Growth Factor Receptor Monoclonal Antibodies by In Vitro Immunization (1992)

Monoclonal antibodies against the high affinity tyrosine kinase Fibroblast Growth Factor (FGF) receptor may help define receptor epitopes involved in FGF binding and signal transduction which mediate coronary and tumor angiogenesis (the development of blood vessels). Monoclonal antibodies against the FGF receptor were made by in vitro immunization. Receptor was PAGE-purified and transblotted to nitrocellulose. The nitrocellulose was dissolved with acetonitrile, and the receptor used as antigen in an in vitro immunization system. Screening for FGF receptor positive clones was done using membrane preparations from Coronary Venular Endothelial Cells (CVEC) expressing the FGF receptor, both by Enzyme Linked Immunosorbent Assay (ELISA) and Western blot. Culture supernatants of several clones tested positive for IgM or IgG monoclonal antibodies against the FGF receptor. Antibodies were affinity purified. Ascites fluid was produced in Balb/c mice primed with Incomplete Freund's adjuvant (IFA). The monoclonal antibodies were also found to be suitable for receptor immunoprecipitation. Immunocytochemistry was done on sections from a variety of species. Further characterization of these antibodies, as well as the production of the remainder of a panel of anti-FGF receptor monoclonal antibodies, is underway.
Insulin Degradation by Acinar Cell Proteases Creates a Dysfunctional Environment for Human Islets Before/After Transplantation: Benefits of α-1 Antitrypsin Treatment (2011)

Culture of impure human islet fractions in the presence of A1AT prevents insulin degradation and improves islet recovery.
Human Antigen-Specific Regulatory T Cells Generated by T Cell Receptor Gene Transfer (2010)

These results support the feasibility of class I-restricted TCR transfer as a promising strategy to redirect the functional properties of Tregs and provide for a more efficacious adoptive cell therapy.
Simulation of exposure and alignment for nanoimprint lithography (2002)

Rigorous electromagnetic simulation with TEMPEST is used to examine the exposure and alignment processes for nano-imprint lithography with attenuating thin-film molds. Parameters in the design of topographical features of the nano-imprint system and material choices of the components are analyzed. The small feature size limits light transmission through the feature. While little can be done with auxiliary structures to attract light into small holes, the use of an absorbing material with a low real part of the refractive index such as silver helps mitigates the problem. Results on complementary alignment marks shows that the small transmission through the metal layer and the vertical separation of two alignment marks create the leakage equivalent to 1 nm misalignment but satisfactory alignment can be obtained by measuring alignment signals over a +/- 30 nm range.
Synovial macrophage depletion with clodronate‐containing liposomes in rheumatoid arthritis (2000)

A single IA administration of clodronate liposomes leads to macrophage depletion and decreased expression of adhesion molecules in the synovial lining in patients with longstanding RA. The procedure is well tolerated, and its therapeutic potential is currently under investigation. The expression of adhesion molecules in the sublining layers reflects ongoing inflammation.
PERYLENE-BASED FLUORESCENT LIQUID CRYSTAL DYE GUEST-HOST MIXTURES (2001)

Abstract We present a study on a series of dye guest-host mixtures using fluorescent perylene-based molecules as the guest dye in an organosiloxane host. These hosts have temperature-independent switching, at room temperature, through 90° for fields of the order of 10 Vrms/μm. Perylene molecules have been grafted onto the organosiloxane moiety via an alkyl spacer producing novel and rugged fluorescent dyes that are readily miscible in the host. Micro-separation of the low molar mass siloxane groups in the mesophases tend to form smectic phases. These planes produce an effective two-dimensional polymer backbonethat engenders the rugged mechanical properties of polymeric liquid crystals onto these low molar mass ferroelectric liquid crystals. In this study we show how the introduction of the dye molecules affects the electro-optic properties of the organosiloxane host.
Oxidative stress‐induced oligomerization inhibits the activity of the non‐receptor tyrosine phosphatase STEP61 (2010)

The neuron-specific tyrosine phosphatase STriatal Enriched Phosphatase (STEP) is emerging as an important mediator of glutamatergic transmission in the brain. STEP is also thought to be involved in the etiology of neurodegenerative disorders that are linked to oxidative stress such as Alzheimer's disease and cerebral ischemia. However, the mechanism by which oxidative stress can modulate STEP activity is still unclear. In this study, we have investigated whether dimerization may play a role in regulating the activity of STEP. Our findings show that STEP(61), the membrane associated isoform, can undergo homodimerization under basal conditions in neurons. Dimerization of STEP(61) involves intermolecular disulfide bond formation between two cysteine residues (Cys 65 and Cys 76 respectively) present in the hydrophobic region at the N-terminus specific to STEP(61). Oxidative stress induced by hydrogen peroxide leads to a significant increase in the formation of dimers and higher-order oligomers of STEP(61). Using two substrates, para-nitrophenylphosphate and extracellular-regulated kinase MAPK we further demonstrate that oligomerization leads to a significant reduction in its enzymatic activity.
ChemInform Abstract: Chemoselective Functionalization of α‐Carbolines at the C‐2, C‐3, C‐4, and C‐6 Positions Using Suzuki—Miyaura Reactions. (2009)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Isolation and characterization of butanol-resistant mutants of Clostridium acetobutylicum (1985)

In a wild-type strain of Clostridium acetobutylicum isolated from soil, solvent production appeared limited by butanol toxicity. Butanol-resistant mutants have been obtained which produced significantly higher solvent concentrations (about 30%) than the wild-type strain. Some other physiological differences were observed between a selected resistant mutant and the wild-type strain at the level of solvent resistance and sporulation.
Omalizumab (Xolair) for treatment of asthma. (2005)

Omalizumab is an immunomodulatory therapy for the control of symptoms in patients with moderate to severe allergic asthma uncontrolled with conventional therapy. Given its high cost, the requirement that it be administered in a physician’s office, and side effects, it is appropriate for use in only a small percentage of patients with asthma.
Spectrum-weighted EEG frequency ("brain-rate") as a quantitative indicator of mental arousal. (2005)

A concept of brain-rate is introduced, defining it as the weighted mean frequency of the EEG spectrum. In analogue to the blood pressure, heart-rate and temperature, used as standard preliminary indicators of corresponding general bodily activations, it is proposed to use the brain-rate as a preliminary indicator of general mental activation (mental arousal) level. In addition, along with the more specific few-band biofeedback parameters (theta-beta ratio, relative beta ratio, etc.), the brain-rate could be effectively used as a general multiband biofeedback parameter.
The nudeotide sequence of the gene for γ-glutamylcysteine synthetase ofEscherichia coli (1986)

The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted a ploypeptide of 518 amino acids with a calculated molecular weight of 58,251. The value is in good agreement with that obtained from gel filtration and SDS/PAGE of GSH I. The initiation codon 5 bp downstream of putative Shine-Dalgarno sequence was an unusual TTG, which encoded methionine. For transcription, two sets of consensus promoter signals(-10 and -35 regions) overlapping each other were identified. The terminator signal shows the favored stem-loop structure with an adequate free energy delta G = -22.80 kcal/mol.
A gene encoding a tyrosine tRNA synthetase is located near Sacs in Bacillus subtilis (1991)

Within the frame of an attempt to sequence the whole Bacillus subtilis genome, a region of 5.5 kbp of the B. subtilis chromosome near the sacS locus has been sequenced. It contains five complete coding sequences, including the sequence of sacY, three unknown CDS and a sequence coding for a tyrosine tRNA synthetase. That the corresponding CDS encodes a functional synthetase has been demonstrated by complementation of an Escherichia coli mutant possessing a thermosensitive tRNA synthetase. Insertion of a kanamycin resistance cassette in the B. subtilis chromosome at the corresponding locus resulted, however, in no apparent phenotype, demonstrating that this synthetase is dispensable. Finally phylogenetic relationships between known tyrosine and tryptophan tRNA synthetases are discussed.
Passive Sensing of Health Outcomes Through Smartphones: Systematic Review of Current Solutions and Possible Limitations (2019)

One relevant outcome of this systematic review is that although smartphones present many advantages for the passive monitoring of users' health and well-being, there is a lack of correlation between smartphone-generated outcomes and clinical knowledge. Moreover, user engagement and motivation are not always modeled as prerequisites, which directly affects user adherence and full validation of such systems.
Enhanced performance of phosphonium based ionic liquids towards 4 electrons oxygen reduction reaction upon addition of a weak proton source (2013)

The oxygen reduction reaction (ORR) in the presence of proton donors of similar proton activities (water, methanol and ethylene glycol), as well as weak and strong acids has been studied in a phosphonium-based IL, [P66614][Cl]. [P66614][Cl]/ethylene glycol mixtures showed better ORR performance than mixtures containing water or methanol in terms of onset potential and current density. This enhancement in performance similar to that produced by weak acids, is attributed to the self-stabilization of ethylene glycol after proton removal; this has been corroborated by calculation of the hydrogen-bond donating ability. Interestingly under these conditions, the oxygen reduction reaction on a glassy carbon electrode follows an overall 4-electron pathway (i.e., two 2-electron processes) which is of importance in the performance of air cathodes in electrochemical devices.
Development of an automated in vitro selection protocol to obtain RNA-based aptamers: identification of a biostable substance P antagonist (2005)

We have developed an automated SELEX (Systematic Evolution of Ligands by EXponential Enrichment) process that allows the execution of in vitro selection cycles without any direct manual intervention steps. The automated selection protocol is designed to provide for high flexibility and versatility in terms of choice of buffers and reagents as well as stringency of selection conditions. Employing the automated SELEX process, we have identified RNA aptamers to the mirror-image configuration (d-peptide) of substance P. The peptide substance P belongs to the tachykinin family and exerts various biologically important functions, such as peripheral vasodilation, smooth muscle contraction and pain transmission. The aptamer that was identified most frequently was truncated to the 44mer SUP-A-004. The mirror-image configuration of SUP-A-004, the so-called Spiegelmer, has been shown to bind to naturally occurring l-substance P displaying a K(d) of 40 nM and to inhibit (IC50 of 45 nM) l-substance P-mediated Ca2+ release in a cell culture assay.
Modification of Porous Silicon Surfaces with Activated Ester Monolayers (2002)

A series of activated esters of undecylenic acid with activated ester end groups (3-nitrophenyl, N-succinimidyl, S-ethyl, and 2,2,2-trifluoroethyl) have been synthesized and covalently linked to a porous silicon (PSi) surface via a thermal hydrosilylation reaction. Diffuse reflectance infrared Fourier transform (DRIFT) or transmission FTIR spectroscopy and X-ray photoelectron spectroscopy (XPS) were used to characterize the monolayers and to follow the course of reactions with primary amines in aqueous solution. It was found that the reaction of the 3-nitrophenyl undecylenate with the PSi surface resulted in significant oxidation of the surface. A control reaction with nitrobenzene confirmed that the arylnitro group was responsible for the surface oxidation and grafting of the nitroaromatic on the surface via a Si−O−N linkage. The N-succinimidyl (NHS) and thioethyl activated ester-modified surfaces formed monolayers with no apparent oxidation of the PSi surface. However, we find that the hydrophobic end groups prevent wetting of the porous structure and further reaction is not possible. Reactions of these groups could be achieved by the addition of 25% (v/v) of ethanol to the aqueous solution of amine, electrochemical partial oxidation of the surface, or preparation of mixed monolayers consisting of mixtures of undecylenic acid and the activated undecylenate ester in a ratio of 5:1 or higher. Under these conditions the NHS group was displaced by ethanolamine or n-butylamine. As expected, exposure of primary amines to the activated S-ethyl thioester did not result in any reaction. However, amide bond formation was observed upon reaction of the methyl ester of cysteine illustrating specificity of this activating group toward cysteine-terminated proteins.
Effects of glycosylation on protein structure and dynamics in ribonuclease B and some of its individual glycoforms (1993)

In an attempt to elucidate the role of carbohydrates on protein structure and dynamics in glycoproteins, ribonuclease B (RNase B), containing a single glycosylation site at Asn34, has been investigated and compared with the enzyme in the unglycosylated form (RNase A). RNase B consists of five glycoforms: Man5GlcNAc2, Man6GlcNAc2, Man7GlcNAc2, Man8GlcNAc2 and Man9GlcNAc2 (Man5-9GlcNAc2). The individual glycoforms Man1GlcNAc2 (synthetic) and Man5GlcNAc2 of RNase B have been studied to ascertain any specific effects of the different oligosaccharides. NMR measurement of amide-proton/deuterium exchange rates shows that glycosylation of the enzyme leads to the protection of amide-proton resonances from solvent exchange for a large number of residues, both in the vicinity of the glycosylation site (e.g. residues 29-34 and 35) and remote from it (e.g. residues 57-61 and 75-76). In addition, residues 10-13, 32, 34 and 35, which are observed to be protected from exchange as a result of glycosylation in the individual glycoforms Man1Glc-NAc2-RNase and Man5GlcNAc2-RNase (when compared with RNase A) are less protected in RNase B. This additional protection in the glycoforms Man1GlcNAc2-RNase and Man5GlcNAc2-RNase may arise from steric hindrance between the oligosaccharide and protein reducing solvent accessibility. The rates of solvent exchange of amide protons for residues 10-13, 32, 34 and 35 are dependent on the oligosaccharide moiety. The average amide-proton/deuterium exchange rate in Man6-9Glc-NAc2-ribonucleases for residues 10-13 and 35 is approximately three times greater than Man5Glc-NAc2-ribonuclease, while for residues 32 and 34 it is approximately 7-11 times greater. CD analysis of RNase A and RNase B revealed the carbohydrate moiety to have a small stabilizing effect (approximately 5 kJ/mol) on the protein.
Constructing Bayesian networks for medical diagnosis from incomplete and partially correct statistics (2000)

The paper discusses several knowledge engineering techniques for the construction of Bayesian networks for medical diagnostics when the available numerical probabilistic information is incomplete or partially correct. This situation occurs often when epidemiological studies publish only indirect statistics and when significant unmodeled conditional dependence exists in the problem domain. While nothing can replace precise and complete probabilistic information, still a useful diagnostic system can be built with imperfect data by introducing domain-dependent constraints. We propose a solution to the problem of determining the combined influences of several diseases on a single test result from specificity and sensitivity data for individual diseases. We also demonstrate two techniques for dealing with unmodeled conditional dependencies in a diagnostic network. These techniques are discussed in the context of an effort to design a portable device for cardiac diagnosis and monitoring from multimodal signals.
Silicon integrated defected ground structures (2010)

In this paper we study the impact of two common defected ground structures fabricated in finite ground coplanar waveguide on silicon and alumina substrates useful for monolithic and hybrid designs. The single defects resonate at 30GHz and have bandwidth differences due to the nature and size of the defect. On-wafer measurements up to 40GHz are included for the silicon designs and show good agreement with simulation.
Recombinant RFB4 single-chain immunotoxin that is cytotoxic towards CD22-positive cells (1997)

Conference Article| May 01 1997 Recombinant RFB4 single-chain immunotoxin that is cytotoxic towards CD22-positive cells E. Mansfield; E. Mansfield *Laboratory of Molecular Biology, DBS, National Cancer Institute, National Institutes of Health, 37/4E16, 37 Convent Drive MSC 4255, Bethesda, MS 20892, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar M. F. Chiron; M. F. Chiron *Laboratory of Molecular Biology, DBS, National Cancer Institute, National Institutes of Health, 37/4E16, 37 Convent Drive MSC 4255, Bethesda, MS 20892, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar P. Amlot; P. Amlot †Department of Immunology, Royal Free Hospital, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar I. Pastan; I. Pastan *Laboratory of Molecular Biology, DBS, National Cancer Institute, National Institutes of Health, 37/4E16, 37 Convent Drive MSC 4255, Bethesda, MS 20892, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar D. J. FitzGerald D. J. FitzGerald ‡ *Laboratory of Molecular Biology, DBS, National Cancer Institute, National Institutes of Health, 37/4E16, 37 Convent Drive MSC 4255, Bethesda, MS 20892, U.S.A. ‡To whom correspondence should be addressed. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1997) 25 (2): 709–714. https://doi.org/10.1042/bst0250709 Article history Received: December 22 1996 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation E. Mansfield, M. F. Chiron, P. Amlot, I. Pastan, D. J. FitzGerald; Recombinant RFB4 single-chain immunotoxin that is cytotoxic towards CD22-positive cells. Biochem Soc Trans 1 May 1997; 25 (2): 709–714. doi: https://doi.org/10.1042/bst0250709 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: PE, Pseudomonas exotoxin A, scFv, single-chain Fv region This content is only available as a PDF. © 1997 Biochemical Society1997 Article PDF first page preview Close Modal You do not currently have access to this content.