gebhart/patrepeval huggingface dataset:
Differential adherence of hydrophobic and hydrophilic Candida albicans yeast cells to mouse tissues (1991)

Using an ex vivo binding assay, we previously demonstrated that yeast cells grown at 37 degrees C display binding specificity in mouse spleen, lymph node, and kidney tissues. In spleen and lymph node tissues, binding was predominantly in regions rich in macrophages. Here, we tested the possibility that hydrophobic and hydrophilic cells bind differentially to host tissues. Hydrophobic and hydrophilic yeast cells of four Candida albicans strains were incubated for 15 min at 4 degrees C with cryostat sections of organs that had been rapidly frozen after removal from BALB/cByJ mice. Unattached cells were removed by washing, and the sections were examined. Hydrophobic cells bound diffusely and abundantly to all tissues, while hydrophilic cell attachment was restricted to specific sites. For example, hydrophobic cells bound to the white and red pulp and the marginal zones in spleens, whereas hydrophilic cells attached primarily to the marginal zones. Hydrophobic yeast cells attached throughout lymph node tissue including paracortical areas, but hydrophilic cell attachment occurred primarily at the subcapsular and trabecular sinuses, EDTA inhibited the adherence of hydrophilic cells but not hydrophobic cells to mouse tissues. Hydrophobic C. albicans strains displaying similar levels of hydrophobicity differed quantitatively in their levels of attachment to kidney and spleen tissues, confirming our earlier observation that surface hydrophobicity is not the sole determinant in adherence to host cells. Other studies have shown that hydrophobic and hydrophilic cells display different virulence characteristics related to their surface properties and that hydrophobic cells are more virulent than hydrophilic cells. Taken together, the present results suggest that the enhanced virulence of hydrophobic cells over hydrophilic cells may be due, in part, to the potential of hydrophobic cells to bind throughout various organs following clearance from the bloodstream.
Exome Capture Sequencing of Adenoma Reveals Genetic Alterations in Multiple Cellular Pathways at the Early Stage of Colorectal Tumorigenesis (2013)

Most of colorectal adenocarcinomas are believed to arise from adenomas, which are premalignant lesions. Sequencing the whole exome of the adenoma will help identifying molecular biomarkers that can predict the occurrence of adenocarcinoma more precisely and help understanding the molecular pathways underlying the initial stage of colorectal tumorigenesis. We performed the exome capture sequencing of the normal mucosa, adenoma and adenocarcinoma tissues from the same patient and sequenced the identified mutations in additional 73 adenomas and 288 adenocarcinomas. Somatic single nucleotide variations (SNVs) were identified in both the adenoma and adenocarcinoma by comparing with the normal control from the same patient. We identified 12 nonsynonymous somatic SNVs in the adenoma and 42 nonsynonymous somatic SNVs in the adenocarcinoma. Most of these mutations including OR6X1, SLC15A3, KRTHB4, RBFOX1, LAMA3, CDH20, BIRC6, NMBR, GLCCI1, EFR3A, and FTHL17 were newly reported in colorectal adenomas. Functional annotation of these mutated genes showed that multiple cellular pathways including Wnt, cell adhesion and ubiquitin mediated proteolysis pathways were altered genetically in the adenoma and that the genetic alterations in the same pathways persist in the adenocarcinoma. CDH20 and LAMA3 were mutated in the adenoma while NRXN3 and COL4A6 were mutated in the adenocarcinoma from the same patient, suggesting for the first time that genetic alterations in the cell adhesion pathway occur as early as in the adenoma. Thus, the comparison of genomic mutations between adenoma and adenocarcinoma provides us a new insight into the molecular events governing the early step of colorectal tumorigenesis.
Audio–Visual Affective Expression Recognition Through Multistream Fused HMM (2008)

Advances in computer processing power and emerging algorithms are allowing new ways of envisioning human-computer interaction. Although the benefit of audio-visual fusion is expected for affect recognition from the psychological and engineering perspectives, most of existing approaches to automatic human affect analysis are unimodal: information processed by computer system is limited to either face images or the speech signals. This paper focuses on the development of a computing algorithm that uses both audio and visual sensors to detect and track a user's affective state to aid computer decision making. Using our multistream fused hidden Markov model (MFHMM), we analyzed coupled audio and visual streams to detect four cognitive states (interest, boredom, frustration and puzzlement) and seven prototypical emotions (neural, happiness, sadness, anger, disgust, fear and surprise). The MFHMM allows the building of an optimal connection among multiple streams according to the maximum entropy principle and the maximum mutual information criterion. Person-independent experimental results from 20 subjects in 660 sequences show that the MFHMM approach outperforms face-only HMM, pitch-only HMM, energy-only HMM, and independent HMM fusion, under clean and varying audio channel noise condition.
High affinity and specificity of dimeric binding of thyroid hormone receptors to DNA and their ligand-dependent dissociation. (1993)

The thyroid hormone receptors are ligand-dependent, DNA binding, trans-acting transcriptional factors belonging to the erbA-related steroid/thyroid hormone receptor superfamily. We report here the high affinity and specificity of dimeric DNA binding of human thyroid hormone receptor-alpha 1 (hTR alpha 1) and hTR beta 1 and the effect of T3 on its DNA binding. Gel mobility shift assay showed that hTR alpha 1 and -beta 1 bind to the rat GH-thyroid hormone response element (TRE) and rat malic enzyme (rME)-TRE as a monomer, dimer, and oligomer in the absence of T3 and bind to an irrelevant DNA sequence from the adenovirus 5 promoter only as a monomer. In competition studies using unlabeled TRE, dimer binding was displaced by lower concentrations of TRE than was the monomer, indicating that the dimeric binding has higher affinity than the monomer binding. These results suggest that the formation of dimers of TR increases the specificity and affinity for the response element. Surprisingly, T3 disrupted dimer binding and increased the intensity of monomer binding to rME-TRE in a dose-dependent manner. This does not occur with the rat GH-TRE. We also demonstrate that this disruption of dimeric binding by T3 occurred on a TRE formed by a direct repeat and not on a palindromic TRE. Furthermore, a mutant hTR beta (Mf), which has no detectable ligand-binding activity because of a glycine to arginine substitution at amino acid 340 in the hormone-binding domain, does not show dissociation from rME-TRE after the addition of T3.(ABSTRACT TRUNCATED AT 250 WORDS)
Therapeutic Monoclonal Antibodies (2011)

Over a hundred years has passed since the discovery of the "magic bullet" serum therapy by Kitasato and Behring, the first ever therapeutic use of antibodies. More than 80 years later, the investigation of immunoglobulin structure and function and the development of cell and molecular biology introduced the production of monoclonal antibodies (MoAbs). In the 35 years since the first process for creating MoAbs was introduced, they have remained the centerpiece of the growing biotechnology and pharmaceutical industry. Herein, I review the history, development, and clinical settings of therapeutic MoAbs that have had a significant impact on life-saving medicine.
Lab-on-a-Foil: microfluidics on thin and flexible films (2010)

This critical review is motivated by an increasing interest of the microfluidics community in developing complete Lab-on-a-Chip solutions based on thin and flexible films (Lab-on-a-Foil). Those implementations benefit from a broad range of fabrication methods that are partly adopted from well-established macroscale processes or are completely new and promising. In addition, thin and flexible foils enable various features like low thermal resistance for efficient thermocycling or integration of easily deformable chambers paving the way for new means of on-chip reagent storage or fluid transport. From an economical perspective, Lab-on-a-Foil systems are characterised by low material consumption and often low-cost materials which are attractive for cost-effective high-volume fabrication of self-contained disposable chips. The first part of this review focuses on available materials, fabrication processes and approaches for integration of microfluidic functions including liquid control and transport as well as storage and release of reagents. In the second part, an analysis of the state of Lab-on-a-Foil applications is provided with a special focus on nucleic acid analysis, immunoassays, cell-based assays and home care testing. We conclude that the Lab-on-a-Foil approach is very versatile and significantly expands the toolbox for the development of Lab-on-a-Chip solutions.
Multiple-access techniques for commercial satellites (1971)

The unique multipoint interconnection capabilities of satellite communication networks place a burden on the communications system designer to achieve highly efficient transmission capacity in the face of a very wide range of network requirements. The ability of various types of multiple-access systems to meet these requirements is the subject of this paper. INTELSAT, recognizing that the frequency division multiplex-frequency modulation, frequencY division multiple access (FDM-FM-FDMA) method it currently uses has advantages in some applications but is not optimum for the multiple-access environment, has supported the development of two-digital multiple-access communications techniques: single channel per carrier frequency division multiple access (SPADE) and time division multiple access (TDMA). These two techniques are discussed in detail and the paper concludes with a presentation of a promising multiple-access technique of the future which includes time division switching on board the satellite.
Sema4D deficiency results in an increase in the number of oligodendrocytes in healthy and injured mouse brains (2009)

Semaphorins, a family of secreted and membrane-bound proteins, are known to function as repulsive axon guidance molecules. Sema4D, a class 4 transmembrane-type semaphorin, is expressed by oligodendrocytes in the central nervous system, but its role is unknown. In this study, the effects of Sema4D deficiency on oligodendrocytes were studied in intact and ischemic brains of adult mice. As observed in previous studies, Sema4D marked by beta-galactosidase in Sema4D mutant mice was localized exclusively on myelin-associated glycoprotein (MAG)-positive oligodendrocytes but not on NG2-positive oligodendrocyte progenitor cells (OPCs). Although there was no difference in the number of the latter cells between Sema4D-deficient and wild-type mice, the number of MAG-positive cells was significantly increased in the cerebral cortex of both nonischemic and postischemic brains of Sema4D-deficient mice. Cell proliferation, observed by using bromodeoxyuridine incorporation, was evident in the MAG-positive cells that developed after cerebral ischemia. These data indicate that Sema4D is involved in oligodendrogenesis during development and during recovery from ischemic injury.
Performance analysis of turbo-coded APSK modulations over nonlinear satellite channels (2006)

This paper investigates the performance of M-ary amplitude-phase shift keying (APSK) digital modulation over typical nonlinear satellite channels. The effect of the satellite nonlinearity is studied, and distortion pre- and post-compensation techniques for coded APSK are presented. Moreover, clock timing, signal amplitude and carrier phase recovery schemes are discussed. For the latter, a new class of non turbo decoder-aided closed-loop phase synchronizers featuring good performance and low complexity is studied. Finally, an end-to-end coded APSK system simulator inclusive of the satellite channel model and synchronization sub-systems is discussed and its performance compared to standard trellis-coded QAM concatenated with Reed-Solomon codes, showing a remarkable gain in both power and spectral efficiency. Coded APSK, recently selected for the new standard -DVB-S2- for digital video broadcasting and interactive broadband satellite services, is shown to represent a powerand spectral-efficient solution for satellite nonlinear channels.
Requirement of a Critical Period of Transcription for Induction of a Late Phase of LTP (1994)

Repeated high-frequency trains of stimuli induce long-term potentiation (LTP) in the CA1 region that persists for up to 8 hours in hippocampal slices and for days in intact animals. This long time course has made LTP an attractive model for certain forms of long-term memory in the mammalian brain. A hallmark of long-term memory in the intact animal is a requirement for transcription, and thus whether the late phase of LTP (L-LTP) requires transcription was investigated here. With the use of different inhibitors, it was found in rat hippocampal slices that the induction of L-LTP [produced either by tetanic stimulation or by application of the cyclic adenosine monophosphate (cAMP) analog Sp-cAMPS (Sp-cyclic adenosine 3′,5′-monophosphorothioate)] was selectively prevented when transcription was blocked immediately after tetanization or during application of cAMP. As with behavioral memory, this requirement for transcription had a critical time window. Thus, the late phase of LTP in the CA1 region requires transcription during a critical period, perhaps because cAMP-inducible genes must be expressed during this period.
Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase (1991)

The nucleotide sequence of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase (PMM; EC 5.4.2.8) was determined. The codon usage in algC in the wobble base position was 90.4% G+C, typical of Pseudomonas genes. The predicted amino acid sequence of phosphomannomutase (PMM) showed homology over a stretch of 112 amino acids in the carboxyl terminus with rabbit muscle phosphoglucomutase (PGM), an enzyme that catalyzes a reaction analogous to that catalyzed by PMM. In addition, a specific amino acid sequence within PMM showed homology with the catalytic site of PGM. DNA sequence analysis of a defective algC gene (algC') cloned from a mutant of P. aeruginosa that lacked PMM activity revealed one point mutation (a C to T transition) in the carboxyl terminus of PMM which resulted in an amino acid change from arginine 420 to cysteine 420. The mutation identified in the algC' gene was not within the regions of homology with PGM. The algC promoter showed significant homology with the promoters of two other P. aeruginosa genes involved in alginate synthesis, algD and algR1. Both the algD and algR1 promoters are activated by the product of the algR1 gene in P. aeruginosa. The upstream region of the algC gene contained a sequence identical to the algD upstream sequence that is known to be the binding site for the AlgR1 protein. Expression of algC was reduced 5.7-fold in an algR1 mutant of P. aeruginosa compared to its isogenic parent strain (lacking the algR1 mutation), suggesting that the algR1 gene product activates the transcription of the algC gene.
ChemInform Abstract: An Alternative Synthesis of the Antineoplastic Nucleoside 4′‐ThioFAC and Its Application to the Synthesis of 4′‐ThioFAG and 4′‐Thiocytarazid. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A Peptide from the Submandibular Glands Modulates Inflammatory Responses (1997)

SGP-T has several biological activities that collectively help explain the ability of the CST-SMG axis to regulate inflammation.
Vaccine induced mumps-like diseases. (1979)

Sixteen cases of parotitis and 2 cases of diabetes mellitus after mumps vaccination have been reported since the introduction of the live attenuated mumps vaccine in the F. R. Germany in the fall of 1976. Due to the post-vaccination incubation of 7 to 10 days, support is given to the assumption that these cases are vaccine induced and not coincidental wild virus infections. The diabetes mellitus cases, however, are too few to be included in this rational.
Thermogravimetry–Mass Spectrometry for Carbon Nanotube Detection in Complex Mixtures (2012)

In spite of the growth of the carbon nanotube (CNT) industry, there are no established analytical methods with which to detect or quantify CNTs in environmental matrices. Given that CNTs have relatively high thermal stabilities, we investigated the use of thermal techniques to isolate and quantify single wall carbon nanotubes (SWCNTs). Test materials included ten types of commercial SWCNTs, representative biological macromolecules (bovine serum albumin and methylcellulose), soot, natural coastal sediments, and SWCNT-amended sediments. Different SWCNTs exhibited widely diverse degradation temperatures, and thermal analytical methods may require SWCNT-type specific parameters. To improve quantification capabilities, evolved gases were monitored by mass spectrometry. SWCNTs produced diagnostic ion ratios reflective of their high carbon and low hydrogen and oxygen contents. Current detection limits are roughly 4 μg(SWCNT) per sample (e.g., 100 μg(SWCNT) g(-1)(sediment) and 40 mg sample), controlled by interfering ions associated with the instrument's non-airtight design. Although future modifications could improve this limitation, the current method is sufficient for quantifying SWCNTs in laboratories and industrial sites where SWCNTs are handled. Furthermore, the method shows promise to distinguish between incidental (e.g., soot) and engineered (e.g., SWCNTs) nanoparticles, which is not possible with current state-of-the-art techniques.
Improving Handwritten Chinese Text Recognition by Unsupervised Language Model Adaptation (2012)

This paper investigates the effects of unsupervised language model adaptation (LMA) in handwritten Chinese text recognition. For no prior information of recognition text is available, we use a two-pass recognition strategy. In the first pass, the generic language model (LM) is used to get a preliminary result, which is used to choose the best matched LMs from a set of pre-defined domains, then the matched LMs are used in the second pass recognition. Each LM is compressed to a moderate size via the entropy-based pruning, tree-structure formatting and fewer-byte quantization. We evaluated the LMA for five LM types, including both character-level and word-level ones. Experiments on the CASIA-HWDB database show that language model adaptation improves the performance for each LM type in all domains. The documents of ancient domain gained the biggest improvement of character-level correct rate of 5.87 percent up and accurate rate of 6.05 percent up.
Regulatory T cells as potential immunotherapy in allergy (2006)

The identification of a role for regulatory T cells in allergic disease has provided a host of new therapeutic possibilities, with the potential prospect of safe and long-term alleviation of allergic diseases.
Alkali Nitrates Molten Salt Modified Commercial MgO for Intermediate-Temperature CO2 Capture: Optimization of the Li/Na/K Ratio (2017)

The promoting effect of alkali nitrates molten salt on the CO2 capture capacity of a commercial MgO was investigated in detail. In particular, the ratio of Li/Na/K nitrates and the loading of the molten salt mixture on the MgO particles were optimized, and the influence of calcination and adsorption temperatures was evaluated. The MgO sample doped with 10 mol % (Li0.3Na0.6K0.1)NO3 was demonstrated to possess the highest CO2 uptake (up to 16.8 mmol g–1), which is the highest value reported for MgO based adsorbents in the literature. The CO2 adsorption/desorption cycling stability was studied using both temperature swing adsorption (TSA) and pressure swing adsorption (PSA). The morphology and structure of the optimized adsorbent, 10 mol % (Li0.3Na0.6K0.1)NO3·MgO, were characterized thoroughly using XRD, SEM, FTIR, and BET analyses. The thermal stability of doped alkali nitrates was investigated via temperature program desorption and XRD analysis, which indicated that the phase status of the molten salts is crucial for the marked improvement in CO2 capture capacity of MgO.
Characterization of the Denaturation and Renaturation of Human Plasma Vitronectin (1996)

Unfolding and refolding of plasma vitronectin appear irreversible under near physiological conditions, with rearrangements of disulfides and self-association to a multimeric form observed as prominent structural alterations which accompany denaturation. A mechanism for the folding reactions of vitronectin has been proposed (Zhuang, P., Blackburn, M. N., and Peterson, C. B.(1996) J. Biol. Chem. 270, 14323-14332) in which vitronectin acquires a partially folded intermediate structure which is highly prone to oligomerize into a multimeric form. Strongly oxidizing conditions adopted for refolding from urea were effective at preventing disulfide rearrangement which disrupts distal disulfides near the C terminus of the protein. Prohibiting disulfide rearrangement under these conditions, however, was not sufficient to achieve reversibility in folding. In contrast, variations in the ionic strength of the refolding medium affect the partitioning of species so that refolded monomers are obtained at high ionic strength, and self-association is precluded. The effects of ionic strength on the partially folded intermediate in the vitronectin folding pathway appear to favor intramolecular hydrophobic collapse to form a stable hydrophobic core for the monomer versus intermolecular hydrophobic interactions which stabilize multimeric vitronectin. Although both ionic and hydrophobic interactions presumably contribute to subunit interfaces within the multimer, the basic heparin-binding region near the C terminus of the protein does not provide binding interactions which are important for self-association of vitronectin.