gebhart/patrepeval huggingface dataset:
Five-year follow-up of 665 Hungarian patients with undifferentiated connective tissue disease (UCTD). (2003)

In our study most of the UCTD patients did not develop a definite CTD, but during the follow-up period we found new clinical and serological manifestations. One-third of the UCTD patients showed progress into different types of specific CTD.
Obesity and Cardiovascular Disease: Pathophysiology, Evaluation, and Effect of Weight Loss (2005)

Obesity is becoming a global epidemic in both children and adults. It is associated with numerous comorbidities such as cardiovascular diseases (CVD), type 2 diabetes, hypertension, certain cancers, and sleep apnea/sleep-disordered breathing. In fact, obesity is an independent risk factor for CVD, and CVD risks have also been documented in obese children. Obesity is associated with an increased risk of morbidity and mortality as well as reduced life expectancy. Health service use and medical costs associated with obesity and related diseases have risen dramatically and are expected to continue to rise. Besides an altered metabolic profile, a variety of adaptations/alterations in cardiac structure and function occur in the individual as adipose tissue accumulates in excess amounts, even in the absence of comorbidities. Hence, obesity may affect the heart through its influence on known risk factors such as dyslipidemia, hypertension, glucose intolerance, inflammatory markers, obstructive sleep apnea/hypoventilation, and the prothrombotic state, in addition to as-yet-unrecognized mechanisms. On the whole, overweight and obesity predispose to or are associated with numerous cardiac complications such as coronary heart disease, heart failure, and sudden death because of their impact on the cardiovascular system. The pathophysiology of these entities that are linked to obesity will be discussed. However, the cardiovascular clinical evaluation of obese patients may be limited because of the morphology of the individual. In this statement, we review the available evidence of the impact of obesity on CVD with emphasis on the evaluation of cardiac structure and function in obese patients and the effect of weight loss on the cardiovascular system.
Comment on “Polarized Raman Study of Aligned Multiwalled Carbon Nanotubes” (2000)

A Comment on the Letter by A. M. Rao, et al., Phys. Rev. Lett. 84, 1820 (2000). The authors of the Letter offer a Reply.Received 25 April 2000DOI:https://doi.org/10.1103/PhysRevLett.85.3544©2000 American Physical Society
Immunotherapeutic modification of Escherichia coli--induced experimental peritonitis and bacteremia by glucan. (1983)

Previous data from our laboratory have demonstrated that glucan administration significantly alters the course of a variety of experimentally induced infectious diseases. In view of the increasing incidence of gram-negative infections, studies were initiated to evaluate the effect of intraperitoneal glucan therapy on Escherichia coli-induced peritonitis and sepsis. Male ICR/Tex mice were injected intraperitoneally with glucan or dextrose on days 5 and 3 prior to intraperitoneal challenge with 1.0 x 10(8) E. coli. Glucan administration resulted in a significant enhancement of survival. Evaluation of the mechanism of protective action of glucan revealed that both the glucan and dextrose control groups showed an equivalent level of blood-borne E. coli at early periods. At 6 hours after challenge the glucan group showed a significant decrease in blood-borne E. coli. In contrast, the dextrose control group demonstrated progressive bacteremia. A significant depression of phagocytic activity occurred in E. coli-infected mice as compared with control mice that were not exposed to the bacterial challenge. The enhancement in phagocytic function observed in glucan-treated control mice was unaltered in E. coli challenged, glucan-treated mice. The possible importance of hyperfunctional macrophages in reduction of mortality from E. coli sepsis was denoted by methyl palmitate-induced reversal of the glucan hyperfunctional state. Methyl palmitate-treated glucan injected mice were not protected against E. coli infection. These data denote that the intraperitoneal administration of glucan significantly modifies the course of E. coli-induced peritonitis and bacteremia due, in part, to glucan-induced enhancement of macrophage function.
Heated humidification in major abdominal surgery. (1984)

The influence of heated humidification on body temperature and postoperative shivering was studied in 30 patients undergoing major intra-abdominal surgery. In the control group (I) the anaesthetic gases, administered in a non-rebreathing system, were humidified by a sponge heat and moisture exchanger. In group II the gases were humidified and heated to 37 degrees C and in group III up to 40 degrees C. Anaesthesia, surface insulation and warming of the infusions were standardized. The temperature was registered at the lower oesophagus and the big toe. Shivering and the feeling of cold were estimated at 15 min intervals postoperatively. A good correlation was found between heat gain during the first hour of recovery, the feeling of cold and intensity of shivering. Intraoperative heat loss was minimal in all groups. Heated humidification had no statistically significant effect on the body temperatures or postoperative shivering and thus provided no additional advantage compared to the control group.
In vitro and in vivo activities of a doxorubicin prodrug in combination with monoclonal antibody beta-lactamase conjugates. (1995)

A cephalosporin derivative of doxorubicin (C-Dox) was evaluated as a prodrug for activation by mAb conjugates of the beta-lactamase from Enterobacter cloacae P99 (beta L; EC 3.5.2.6). The conjugates consisted of beta L and the F(ab') fragments of either of the mAbs L6, P1.17, or 96.5. L6 binds to antigens on a variety of carcinomas, including the two lung adenocarcinoma cell lines H2981 and H2987 used in this study. 96.5 binds to the melanoma-associated antigen p97, and P1.17 was used for the control conjugate. C-Dox was found to be less cytotoxic to three different tumor cell lines in vitro compared to the parent drug doxorubicin (Dox). Immunospecific activation took place when the cells were pretreated with beta L conjugates that could bind to antigens on the tumor cells. In vivo toxicity and pharmacokinetics studies in athymic female nu/nu mice revealed that C-Dox was at least 7-fold less toxic than Dox (on a molar basis), despite the fact that a > or = 320-fold greater area-under-the-curve (blood concentration versus time) of C-Dox compared to Dox was obtained 0-2 h after administration of the two agents. Pharmacokinetic studies at maximum tolerated doses in mice bearing xenografts of either H2981 or H2987 revealed that the intratumoral levels of Dox after treatment with L6-beta L in combination with C-Dox were higher than were obtained by either systemic treatment with Dox or a combination of P1.17-beta L and C-Dox. This finding suggested that the conversion of C-Dox to Dox was tumor specific and dependent on the presence of the targeted antigen. Furthermore, the best antitumor activity against both H2981 and H2987 tumors was obtained by treatment with L6-beta L and C-Dox compared to P1.17-beta L and C-Dox or Dox alone. Thus, higher levels of Dox corresponded to greater therapeutic effects in both of the tumor models studied.
Of urfs and orfs : a primer on how to analyze devised amino acid sequences (1986)

Searching through data banks So you found something! Clocks and trees Characterizing an ORF: structure-function considerations Synthetic peptide antigens.
Tolerance of single, but not multiple, amino acid replacements in antibody VH CDR 2: a means of minimizing B cell wastage from somatic hypermutation? (1996)

Mutations in the heavy chain complementarity determining region 2 (CDR2) of the phosphocholine-specific T15 Ab can have a dramatic effect on the ability of the Ab to bind Ag. A panel of multisite mutants that had lost detectable binding to phosphocholine-containing Ags was previously created by saturation mutagenesis of the CDR2 region of T15. Based on the predicted importance of amino acid changes represented in the multisite mutants, we have created single-site mutations, yielding a panel of Abs with which to test 17 of the 19 CDR2 residues. Of the 17 positions examined, only one, Arg52, is intolerant to change, yielding a nonbinder phenotype even with conservative amino acid replacement. Mutation at two other sites, Ala50 and Tyr55, can yield a nonbinder phenotype depending on the amino acid replacement. Single-site mutations of the remaining 14 positions allowed retention of binding ability. Thus, except for positions 50, 52, and 55, multiple mutations must be introduced into the CDR2 region to create a nonbinder phenotype. We provide a newly refined model of T15, illustrating the structure and the interactions of the CDR2 region. Our results imply that introduction of point mutations would not normally delete Ag-binding ability until two or more mutations had accumulated. This would minimize potentially harmful effects of somatic mutation on Ig V region genes and improve the chance of survival for an Ab such as T15, which in its unmutated form is already well suited to bind Ag.
Experimental autoimmune prostatitis induces chronic pelvic pain (2008)

Pain is the hallmark of patients with chronic prostatitis (CP) and chronic pelvic pain syndrome (CPPS). Despite numerous hypotheses, the etiology and pathogenesis remain unknown. To better understand CP/CPPS, we used a murine experimental autoimmune prostatitis model to examine the development, localization, and modulation of pelvic pain. Pelvic pain was detected 5 days after antigen instillation and was sustained beyond 30 days, indicating the development of chronic pain. The pain was attenuated by lidocaine treatment into the prostate, but not into the bladder or the colon, suggesting that pain originated from the prostate. Experimental autoimmune prostatitis histopathology was confined to the prostate with focal periglandular inflammatory infiltrates in the ventral, dorsolateral, and anterior lobes of the mouse prostate. Inflammation and pelvic pain were positively correlated and increased with time. Morphologically, the dorsolateral prostate alone showed significantly increased neuronal fiber distribution, as evidenced by increased protein gene product 9.5 expression. Pelvic pain was attenuated by treatment with the neuromodulator gabapentin, suggesting spinal and/or supraspinal contribution to chronic pain. These results provide the basis for identifying mechanisms that regulate pelvic pain and the testing of therapeutic agents that block pain development in CP/CPPS.
Influence of Nanoparticle Addition on the Properties of Wormlike Micellar Solutions (2008)

The addition of positively charged, 30 nm diameter silica nanoparticles to cationic wormlike micellar solutions of cetyltrimethylammonium bromide and sodium nitrate is studied using a combination of rheology, small angle neutron scattering, dynamic light scattering, and cryo-transmission electron microscopy. The mixtures are single phase up to particle volume fractions of 1%. The addition of like-charged particles significantly increases the wormlike micelle (WLM) solution's zero shear rate viscosity, longest relaxation time, and storage modulus. The changes are hypothesized to originate from a close association of the particles with the micellar mesh. Small angle neutron scattering measurements with contrast matching demonstrate associations between particles mitigated by the WLMs. The effective interparticle interactions measured by SANS can explain the observed phase behavior. Dynamic light scattering measurements confirm the dynamic coupling of the particles to the micellar mesh.
Comparative Diet Analysis Of Fish Species Commonly Consumed By Managed And Free-Ranging Bottlenose Dolphins (Tursiops Truncatus) (2013)

Managed bottlenose dolphins (Tursiops truncatus) cared for by the Chicago Zoological Society’s Brookfield Zoo Seven Seas exhibit in Brookfield, Illinois, are fed primarily whole boney fish, a diet similar to that of free-ranging dolphins. However, the diets of the two dolphin populations differ in that managed dolphins are fed primarily frozen, stored, and thawed Canadian capelin (Mallotus villosus) and Atlantic herring (Clupea harengus) and free-ranging dolphins commonly consume a wide variety of live fish. Dolphins in Sarasota Bay, Florida, a coastal region where the Brookfield Zoo dolphins originated from, feed on numerous fish species, including pinfish (Lagodon rhomboides), pigfish (Orthopristis chrysoptera), toad fish (Opsanus beta) and striped mullet (Mugil cephalus), among many others. Considering that the nutrient composition of fish differs with species, marine location, season, and storage and handling methods, this is the first preliminary comparative analysis identifying nutrient variations in the fish commonly fed to one population of managed dolphins versus those consumed by a comparable population of free-ranging dolphins in one region of the world. The study results confirm differences particularly in dry matter content (range 19% capelin to 34% mullet), crude protein (range 53% pinfish to 69% capelin) , crude fat (range 11% pigfish to 22% herring), and individual minerals, across fish species. Future research is necessary to assess dolphin health in relation to diet nutrient composition by comparing clinical findings in either managed or free-ranging dolphins to the results of this diet analysis study.
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity (2008)

We outline a powerful method for the directed evolution of integral membrane proteins in the inner membrane of Escherichia coli. For a mammalian G protein-coupled receptor, we arrived at a sequence with an order-of-magnitude increase in functional expression that still retains the biochemical properties of wild type. This mutant also shows enhanced heterologous expression in eukaryotes (12-fold in Pichia pastoris and 3-fold in HEK293T cells) and greater stability when solubilized and purified, indicating that the biophysical properties of the protein had been under the pressure of selection. These improvements arise from multiple small contributions, which would be difficult to assemble by rational design. In a second screen, we rapidly pinpointed a single amino acid substitution in wild type that abolishes antagonist binding while retaining agonist-binding affinity. These approaches may alleviate existing bottlenecks in structural studies of these targets by providing sufficient quantities of stable variants in defined conformational states.
Neutrophil-induced injury of rat pulmonary alveolar epithelial cells. (1986)

The damage to pulmonary alveolar epithelial cells that occurs in many inflammatory conditions is thought to be caused in part by phagocytic neutrophils. To investigate this process, we exposed monolayers of purified rat alveolar epithelial cells to stimulated human neutrophils and measured cytotoxicity using a 51Cr-release assay. We found that stimulated neutrophils killed epithelial cells by a process that did not require neutrophil-generated reactive oxygen metabolites. Pretreatment of neutrophils with an antibody (anti-Mo1) that reduced neutrophil adherence to epithelial cells limited killing. Although a variety of serine protease inhibitors partially inhibited cytotoxicity, we found that neutrophil cytoplasts, neutrophil lysates, neutrophil-conditioned medium, purified azurophilic or specific granule contents, and purified human neutrophil elastase did not duplicate the injury. We conclude that stimulated neutrophils can kill alveolar epithelial cells in an oxygen metabolite-independent manner. Tight adherence of stimulated neutrophils to epithelial cell monolayers appears to promote epithelial cell killing.
Microencapsulated Osmium Tetraoxide. A New Recoverable and Reusable Polymer-Supported Osmium Catalyst for Dihydroxylation of Olefins (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTMicroencapsulated Osmium Tetraoxide. A New Recoverable and Reusable Polymer-Supported Osmium Catalyst for Dihydroxylation of OlefinsSatoshi Nagayama, Masahiro Endo, and Shū KobayashiView Author Information Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan, and Department of Applied Chemistry, Faculty of Science, Science University of Tokyo (SUT), Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan Cite this: J. Org. Chem. 1998, 63, 18, 6094–6095Publication Date (Web):August 13, 1998Publication History Received12 June 1998Published online13 August 1998Published inissue 1 September 1998https://pubs.acs.org/doi/10.1021/jo981127yhttps://doi.org/10.1021/jo981127yrapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views3129Altmetric-Citations136LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Catalysts,Hydrocarbons,Osmium,Polymers,Solvents Get e-Alerts
Cinical observation for the treatment of children RSV pneumonia with ke-an oral liquid and interferon (2006)

Objective
To observe and study the curative of ke-an oral liquid and interferon on treating children with RSV pneumonia.


Methods
The treatment group was treated with ke-an oral liquid and interferon, the control group with phenergon syrup and interferon. Symptoms and signs were observed and noted for children every day.


Results
The effective rate in the tretment group was 94. 17%, that in the control group was 86. 04(P<0. 001). All the observation indexes of the treatment group are better than the control group.


Conclusion
The effects of ke-an oral liquid and interferon on treating children with RSV pneumonia is definite, it is suitable for clinical treatment.


Key words: 
ke-an oral liguid; interferon; children RSV pneumonia; treatment; clinical observation
Three-Dimensional Pattern Recognition from Molecular Distance Minimization (1996)

A procedure optimizing the alignment of two three-dimensional molecules is presented. It uses atomic numbers, positions and radii, and optionally computed atomic charges. Neither bonds nor connectivities are required. The dissimilarity between two molecules is measured with a molecular distance, which is minimized under all rotations and translations with a Newton-like algorithm. This molecular distance is the usual norm of a two-components vector. One of the components is the electronic molecular distance, and the other is the protonic molecular distance. Both the electronic and the protonic component are computed with the same algorithm, which assumes a homogeneous spheres model. The resulting minimized norm is shown to be an intrinsic molecular distance. When a family of more than two compounds is involved, the intrinsic molecular distances matrix of the family is built. Various applications are presented, including comparisons of X-ray crystallographic data compounds, maximal common 3D-substructure searching, comparisons of geometry and charge calculations, and quantitative chirality measurement. The optimal alignments are more easily obtained when large atomic radii are selected. The charge calculation algorithm have only a little influence on the results.
Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis. (1988)

The pathway of sterol biosynthesis is highly conserved in all eucaryotic cells. We demonstrated structural and functional conservation of the rate-limiting enzyme of the mammalian pathway, 3-hydroxy-3-methyl-glutaryl coenzyme A reductase (HMG-CoA reductase), between the yeast Saccharomyces cerevisiae and humans. The amino acid sequence of the two yeast HMG-CoA reductase isozymes was deduced from DNA sequence analysis of the HMG1 and HMG2 genes. Extensive sequence similarity existed between the region of the mammalian enzyme encoding the active site and the corresponding region of the two yeast isozymes. Moreover, each of the yeast isozymes, like the mammalian enzyme, contained seven potential membrane-spanning domains in the NH2-terminal region of the protein. Expression of cDNA clones encoding either hamster or human HMG-CoA reductase rescued the viability of hmg1 hmg2 yeast cells lacking this enzyme. Thus, mammalian HMG-CoA reductase can provide sufficient catalytic function to replace both yeast isozymes in vivo. The availability of yeast cells whose growth depends on human HMG-CoA reductase may provide a microbial screen to identify new drugs that can modulate cholesterol biosynthesis.
Methicillin-Resistant Staphylococcus aureus Colonization at Different Body Sites: a Prospective, Quantitative Analysis (2011)

We quantified methicillin-resistant Staphylococcus aureus (MRSA) carriage. The greater the log(10) count in samples from the nares, the greater the likelihood that other body sites had been colonized. Log(10) counts among body sites were correlated. The greatest sensitivity value (98%) was determined for the combined results from 2 sites: the nares and the groin.
Bioresorbable Glass Fibres Facilitate Peripheral Nerve Regeneration (2005)

This is a proof of principle report showing that fibres of Bioglass 45S5 can form a biocompatible scaffold to guide regrowing peripheral axons in vivo. We demonstrate that cultured rat Schwann cells and fibroblasts grow on Bioglass fibres in vitro using SEM and immunohistochemistry, and provide qualitative and quantitative evidence of axonal regeneration through a Silastic conduit filled with Bioglass fibres in vivo (across a 0.5 cm interstump gap in the sciatic nerves of adult rats). Axonal regrowth at 4 weeks is indistinguishable from that which occurs across an autograft. Bioglass fibres are not only biocompatible and bioresorbable, which are absolute requirements of successful devices, but are also amenable to bioengineering, and therefore have the potential for use in the most challenging clinical cases, where there are long inter-stump gaps to be bridged.
<title>Programmable 128 x 128 ferroelectric liquid crystal spatial light modulator compact correlator</title> (1993)

This paper details a compact two-dimensional optical correlator based on 128 X 128 ferroelectric liquid crystal spatial light modulators in both the image and filter planes. A complete description of this compact correlator is given. The correlator is fully programmable and performs automatic pattern recognition functions at 500 frames per second. Key design parameters and results of performance analysis are presented.