gebhart/patrepeval huggingface dataset:
Tumor-Specific T Cell Activation by Recombinant Immunoreceptors: CD3ζ Signaling and CD28 Costimulation Are Simultaneously Required for Efficient IL-2 Secretion and Can Be Integrated into One Combined CD28/CD3ζ Signaling Receptor Molecule (2004)

Andreas Hombach, Anja Wieczarkowiecz, Thomas Marquardt, Claudia Heuser, Loretta Usai, Christoph Pohl, Barbara Seliger, and Hinrich Abken. Tumor-Specific T Cell Activation by Recombinant Immunoreceptors: CD3ζ Signaling and CD28 Costimulation Are Simultaneously Required for Efficient IL-2 Secretion
Vascular wall resident progenitor cells: a source for postnatal vasculogenesis (2006)

Here, we report the existence of endothelial precursor (EPC) and stem cells in a distinct zone of the vascular wall that are capable to differentiate into mature endothelial cells, hematopoietic and local immune cells, such as macrophages. This zone has been identified to be localized between smooth muscle and adventitial layer of human adult vascular wall. It predominantly contains CD34-positive (+) but CD31-negative (-) cells, which also express VEGFR2 and TIE2. Only few cells in this zone of the vascular wall are positive for CD45. In a ring assay using the fragments of human internal thoracic artery (HITA), we show here that the CD34+ cells of the HITA-wall form capillary sprouts ex vivo and are apparently recruited for capillary formation by tumor cells. New vessels formed by these vascular wall resident EPCs express markers for angiogenically activated endothelial cells, such as CEACAM1, and also for mature endothelial cells, such as VE-cadherin or occludin. Vascular wall areas containing EPCs are found in large and middle sized arteries and veins of all organs studied here. These data suggest the existence of a ;vasculogenic zone' in the wall of adult human blood vessels, which may serve as a source for progenitor cells for postnatal vasculogenesis, contributing to tumor vascularization and local immune response.
Treatment of stroke in rat with intracarotid administration of marrow stromal cells (2001)

MSC injected intra-arterially are localized and directed to the territory of the middle cerebral artery, and these cells foster functional improvement after cerebral ischemia.
Cisphosphorylated tau as the earliest detectable pathogenic conformation in Alzheimer disease, offering novel diagnostic and therapeutic strategies (2013)

After protein phosphorylation on certain serine or threonine residues preceding a proline (pSer/Thr-Pro), the function of certain phosphorylated protein is further regulated by cis-trans conformational change. Due to the lack of any tool to detect such two conformations in cells, however, it is not even known whether any cis or trans conformation exists in vivo, not to mention their conformation-specific functions or regulation. We developed a novel peptide chemistry technology to generate the first pair of antibodies that can distinguish cis from trans pThr231-Pro tau. Cis, but not trans, pThr231-tau appears early in mild cognitive impairment (MCI) neurons and further accumulates in only degenerating neurons as Alzheimer disease (AD) progresses, localizing to dystrophic neurites, which are known to correlate well with memory loss. Unlike trans p-tau, the cis cannot promote microtubule assembly, and is more resistant to dephosphorylation and degradation and more prone to aggregation. Pin1 accelerates cis to trans isomerization to prevent tau pathology in AD. Thus, during MCI and AD development, cis pThr231-Pro tau is the earliest detectable pathogenic tau conformation and antibodies and vaccines against the pathogenic cis p-tau may be used for the early diagnosis and treatment of AD. These findings offer in vivo approach to study conformational regulation of Pro-directed phosphorylation signaling.
Biotechnological production of succinic acid: current state and perspectives (2012)

Abstract Succinic acid has multiple practical applications (e.g. synthesis of 1,4‐butanediol, tetrahydrofuran, gamma‐butyrolactone, and as a monomer of some biodegradable polymers). Bio‐based succinic acid is a potential substitute for current petrochemical production. Facing a shortage of crude oil supply and sharply rising oil prices, biological production of succinic acid from abundant and available biomass has become a topic of worldwide interest. Although great progress has been made in recent decades, much needs to be developed further in order to achieve economic viability. This paper reviews developments in technology and updates research progress of biosuccinate production, including pathways, micro‐organisms, culture conditions, as well as integrated production with other high‐value‐added products. Finally, strategies are proposed for successful commercialization of fermentative succinic acid production. © 2012 Society of Chemical Industry and John Wiley &amp; Sons, Ltd
Scaling laws, pressure anisotropy, and thermodynamic effects for blackbody radiation in a finite cavity (2014)

Finite-size effects for blackbody radiation in a cavity are revealed. We find out simple scaling laws for thermodynamic functions of blackbody radiation in a finite cavity of arbitrary shape and predict the anisotropy of blackbody radiation pressure in asymmetric cavities. Special thermodynamic effects accompanying cavities merge are discussed.
Fate of a Modified Fenestration of Atrial Septal Occluder Device after Transcatheter Closure of Atrial Septal Defects in Elderly Patients (2011)

The modified fenestration of the ASO decreased significantly in size at follow-up. Applying this technique to selected patients judged to be at risk for ASD closure avoids acute decompensation and allows gradual diminuition of right ventricular volume overload during mid-term follow-up.
Analysis of T cell receptor beta chain CDR3 size using RNA extracted from formalin fixed paraffin wax embedded tissue. (1997)

RNA and DNA were isolated from formalin fixed paraffin wax embedded enteropathy associated T cell lymphoma tissue. Detailed analysis of clonality can be carried out by a non-radioactive method of CDR3 spectratyping.
Nickel(0)-catalyzed cycloaddition of diynes and carbon dioxide to give bicyclic .alpha.-pyrones (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNickel(0)-catalyzed cycloaddition of diynes and carbon dioxide to give bicyclic .alpha.-pyronesTetsuo Tsuda, Shohei Morikawa, Ritsuo Sumiya, and Takeo SaegusaCite this: J. Org. Chem. 1988, 53, 14, 3140–3145Publication Date (Print):July 1, 1988Publication History Published online1 May 2002Published inissue 1 July 1988https://pubs.acs.org/doi/10.1021/jo00249a003https://doi.org/10.1021/jo00249a003research-articleACS PublicationsRequest reuse permissionsArticle Views980Altmetric-Citations140LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Inflammation-induced tumorigenesis in the colon is regulated by caspase-1 and NLRC4 (2010)

Chronic inflammation is a known risk factor for tumorigenesis, yet the precise mechanism of this association is currently unknown. The inflammasome, a multiprotein complex formed by NOD-like receptor (NLR) family members, has recently been shown to orchestrate multiple innate and adaptive immune responses, yet its potential role in inflammation-induced cancer has been little studied. Using the azoxymethane and dextran sodium sulfate colitis-associated colorectal cancer model, we show that caspase-1-deficient (Casp1(-/-)) mice have enhanced tumor formation. Surprisingly, the role of caspase-1 in tumorigenesis was not through regulation of colonic inflammation, but rather through regulation of colonic epithelial cell proliferation and apoptosis. Consequently, caspase-1-deficient mice demonstrate increased colonic epithelial cell proliferation in early stages of injury-induced tumor formation and reduced apoptosis in advanced tumors. We suggest a model in which the NLRC4 inflammasome is central to colonic inflammation-induced tumor formation through regulation of epithelial cell response to injury.
Cannabis and Cannabinoids: Pharmacology and Rationale for Clinical Use (1997)

There are two types of cannabinoid receptor, CB1 and CB2, both coupled to Gi/o proteins. CB1 receptors are present on certain central and peripheral neurons whereas CB2 receptors have been found mainly in immune cells. Endogenous agonists for cannabinoid receptors have also been identified. These recent discoveries have prompted the development of selective CB1 and CB2 receptor ligands. They have also stimulated renewed interest in the clinical potential of these ligands. Two cannabinoids, Δ9-tetrahydrocannabinol and nabilone, are already prescribed in the UK or USA as anti-emetics or to stimulate appetite. Other possible uses for cannabinoid receptor agonists include pain relief, suppression of muscle spasticity (and pain) associated with multiple sclerosis or spinal injury, management of glaucoma, bronchial asthma and inflammatory disorders, and modulation of immune function. Cannabinoid receptor antagonists may also have clinical applications, for example in reducing memory deficits associated with ageing or neurological diseases. It is important that further preclinical and clinical investigations are carried out so that the therapeutic potential of cannabinoids can be exploited to the full.
A novel glucose-responsive element in the human insulin gene functions uniquely in primary cultured islets (1998)

Insulin gene transcription is limited to the beta cells within the mammalian pancreas and, like insulin secretion, is regulated by glucose. Our previous studies in primary cultured beta cells suggested the presence of a strong glucose-responsive enhancer element between base pairs -341 and -260 of the human insulin promoter, the same region in which a transcriptional repressor had been identified in beta-cell tumor lines. In an attempt to map these promoter activities and resolve these conflicting data, we designed minienhancer constructs spanning this region, and tested them in primary cultured and immortalized cells. One sequence, the Z element (base pairs -292 to -243), functions as both a potent glucose-responsive transcriptional enhancer in primary cultured islet cells and as a transcriptional repressor in immortalized beta and nonbeta cells and in primary fibroblasts. In addition, the Z element binds a novel glucose-responsive protein complex that is found in the nuclei of primary cultured islet cells, but not in the nuclei of tumor cells or primary cultured fibroblasts. These data demonstrate a critical role for the Z element in human insulin gene transcription and its regulation by glucose.
1H NMR studies of echistatin in solution (1991)

Two-dimensional 1H-NMR methods have been used to obtain complete proton resonance assignments for the 49-residue protein echistatin from the viper Echis carinatus. The protein in solution contains only a small amount of regular secondary structure with four very short beta-strands. These beta-strands form two short segments of antiparallel beta-sheet, as evidenced by the observed cross-strand NOE. The first two strands are connected with a tight reverse turn, whereas the remaining two strands are linked together by an 11-residue loop forming a so-called hairpin. The tripeptide unit Arg-Gly-Asp, responsible for the binding of echistatin to the fibrinogen receptor glycoprotein GPIIb/IIIa, is located at the tip of this very hydrophilic loop.
Batf3 Deficiency Reveals a Critical Role for CD8α + Dendritic Cells in Cytotoxic T Cell Immunity (2008)

Although in vitro observations suggest that cross-presentation of antigens is mediated primarily by CD8alpha+ dendritic cells, in vivo analysis has been hampered by the lack of systems that selectively eliminate this cell lineage. We show that deletion of the transcription factor Batf3 ablated development of CD8alpha+ dendritic cells, allowing us to examine their role in immunity in vivo. Dendritic cells from Batf3-/- mice were defective in cross-presentation, and Batf3-/- mice lacked virus-specific CD8+ T cell responses to West Nile virus. Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3-/- mice. These results suggest an important role for CD8alpha+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection.
Severe fibrosis in hepatitis C virus-infected patients is associated with increased activity of the mannan-binding lectin (MBL)/MBL-associated serine protease 1 (MASP-1) complex (2006)

Mannan-binding lectin (MBL) binds microorganisms via interactions with glycans on the target surface. Bound MBL subsequently activates MBL-associated serine protease proenzymes (MASPs). A role for MBL in hepatitis C virus (HCV) infection had been indicated by previous studies examining MBL levels and polymorphisms in relation to disease progression and response to treatment. We undertook this study to investigate a possible relationship between disease progression and functional MBL/MASP-1 complex activity. A functional assay for MBL/MASP-1 complex activity was employed to examine serum samples from patients with chronic HCV infection, non-HCV liver disease and healthy controls. Intrapatient consistency of MBL/MASP-1 complex activity levels was assessed in sequential samples from a subgroup of patients. Median values of MBL/MASP-1 complex activity were higher in sera from patients with liver disease compared with healthy controls. MBL/MASP-1 complex activity levels correlate with severity of fibrosis after adjusting for confounding factors (P = 0.003). MBL/MASP-1 complex activity was associated more significantly with fibrosis than was MBL concentration. The potential role of MBL/MASP-1 complex activity in disease progression is worthy of further study to investigate possible mechanistic links.
Positively Cooperative Sites for Drug Transport by P‐Glycoprotein with Distinct Drug Specificities (1997)

In this paper, we show that P-glycoprotein contains two distinct sites for drug binding and transport, and that, unexpectedly, these sites interact in a positively cooperative manner. The kinetics of transport of rhodamine 123 and Hoechst 33342 in isolated P-glycoprotein-rich plasma membrane vesicles from Chinese hamster ovary CH(R)B30 cells were followed by continuous fluorescence monitoring. Each substrate stimulated P-glycoprotein-mediated transport of the other. Colchicine and quercetin stimulated rhodamine 123 transport and inhibited Hoechst 33342 transport. In contrast, anthracyclines such as daunorubicin and doxorubicin stimulated Hoechst 33342 transport and inhibited rhodamine 123 transport. Vinblastine, actinomycin D, and etoposide inhibited transport of both dyes. The results are consistent with a functional model of P-glycoprotein containing at least two positively cooperative sites (H site and R site) for drug binding and transport. This model is consistent with earlier observations of competitive and non-competitive effects of P-glycoprotein substrates and chemosensitizers. Such a two-site model may be fundamental to multidrug transport by P-glycoprotein, and it may be a feature common to other ATP-dependent transporters belonging to the ATP-binding cassette superfamily.
Beyond Feeling: Chronic Pain Hurts the Brain, Disrupting the Default-Mode Network Dynamics (2008)

Chronic pain patients suffer from more than just pain; depression and anxiety, sleep disturbances, and decision-making abnormalities (Apkarian et al., 2004a) also significantly diminish their quality of life. Recent studies have demonstrated that chronic pain harms cortical areas unrelated to pain (Apkarian et al., 2004b; Acerra and Moseley, 2005), but whether these structural impairments and behavioral deficits are connected by a single mechanism is as of yet unknown. Here we propose that long-term pain alters the functional connectivity of cortical regions known to be active at rest, i.e., the components of the "default mode network" (DMN). This DMN (Raichle et al., 2001; Greicius et al., 2003; Vincent et al., 2007) is marked by balanced positive and negative correlations between activity in component brain regions. In several disorders, however this balance is disrupted (Fox and Raichle, 2007). Using well validated functional magnetic resonance imaging (fMRI) paradigms to study the DMN (Fox et al., 2005), we investigated whether the impairments of chronic pain patients could be rooted in disturbed DMN dynamics. Studying with fMRI a group of chronic back pain (CBP) patients and healthy controls while executing a simple visual attention task, we discovered that CBP patients, despite performing the task equally well as controls, displayed reduced deactivation in several key DMN regions. These findings demonstrate that chronic pain has a widespread impact on overall brain function, and suggest that disruptions of the DMN may underlie the cognitive and behavioral impairments accompanying chronic pain.
Contruction of Defined galE Mutants of Salmonella for Use as Vaccines (1987)

We describe the molecular cloning of the gal operon of Salmonella typhimurium LT2 and the localization of the gal promoter and the genes galE, galT, and galK. The order of the genes and the direction of transcription was the same as in Escherichia coli K12. A restriction enzyme map of the operon was obtained, and the approximate termini of the gal genes were located by using transposon insertion mutagenesis and minicell analysis. We constructed a plasmid that contained a defined 0.4-kilobase deletion in the galE but still expressed galT and galK activities from the gal promoter. This galE deletion was recombined into the chromosomal gal operons of S. typhimurium and Salmonella typhi Ty2. The resulting strains were vigorous, nonreverting galE mutants that were sensitive to galactose-induced lysis at 0.2 mM galactose. The S. typhimurium galE derivatives were avirulent and protective in mice.
Facilitation of beta-tricalcium phosphate-induced alveolar bone regeneration by platelet-rich plasma in beagle dogs: a histologic and histomorphometric study. (2005)

The increase in bone density facilitated by grafting with a combination of Cerasorb and PRP requires thorough study in humans.
Transfusion‐transmitted hepatitis E caused by apparently indigenous hepatitis E virus strain in Hokkaido, Japan (2004)

The patient was infected with HEV via transfused blood from a volunteer donor. A potential risk of posttransfusion hepatitis E should be considered even in nonendemic countries.
High molecular weight insulin-like growth factor binding protein complex (1989)

In the human circulation, the insulin-like growth factors (IGFs) circulate as part of a growth hormone-dependent 125- to 150-kDa complex. This complex has been postulated to contain, in addition to IGFs and one or more IGF-binding proteins, an acid-labile subunit (ALS) which does not itself bind IGFs. In this study, the ALS has been purified 1600-fold from human serum, and its binding properties have been examined. Fresh serum was fractionated on DEAE-Sephadex, and active fractions (determined by radioimmunoassay) were purified by affinity chromatography on an IGF-agarose column saturated with the plasma IGF-binding protein BP-53. After further high performance anion exchange chromatography, an ALS preparation was obtained which contained only an 84-86-kDa protein doublet, converting to a single 70-kDa band on N-glycanase treatment, and having an amino-terminal sequence unrelated to IGF-binding proteins or receptors. Pure ALS formed a complex with BP-53 (Ka approximately 5 x 10(8) M-1), immunoprecipitable by anti-BP-53 antiserum, only in the presence of IGF-I or IGF-II. This complex appeared at approximately 150 kDa on high performance gel chromatography. Pure ALS had no intrinsic IGF-binding activity and no effect on the binding of IGF-I or IGF-II to BP-53. These studies suggest that formation of the high molecular weight IGF-binding protein complex requires ALS, BP-53, and IGF.
Molecular cloning, sequence and characterization of a novel streptococcal phosphoglycerate dehydrogenase gene (2000)

The nucleotide sequence of a Streptococcus mutans serA gene that encodes D-3-phosphoglycerate dehydrogenase has been determined. The gene consisted of 1308-bp nucleotides coding for a 436-amino-acid polypeptide (48,546 Da). The deduced amino acid sequence showed a 66% identity with SerA from Bacillus subtilis and possessed specific residues (G-R-P-N-V-G) in the coenzyme-binding domain, alpha B helix. Recombinant streptococcal SerA was expressed using pMAL-c2 expression vector and purified by amylose resin affinity chromatography and DEAE-Sephacel column chromatography. This SerA enzyme catalyzed detectable reduction of alpha-ketoglutarate to 2-hydroxyglutaric acid. These findings indicate that the novel streptococcal phosphoglycerate dehydrogenase, SerA, is a member of a D-isomer-specific family of 2-hydroxyacid dehydrogenases.
FOLLOW-UP OF THE MACINTOSH ARTHROPLASTY OF THE KNEE JOINT (1972)

Journal Article FOLLOW-UP OF THE MACINTOSH ARTHROPLASTY OF THE KNEE JOINT Get access J. D. JESSOP, J. D. JESSOP †Present appointment: Consultant in Physical Medicine and Rheumatology, University Hospital of WalesCardiff Search for other works by this author on: Oxford Academic PubMed Google Scholar C.J MOORE C.J MOORE ‡ Consultant Orthopaedic Surgeon, Chelmsford and St. Helena Group Hospitals Search for other works by this author on: Oxford Academic PubMed Google Scholar Rheumatology, Volume 11, Issue 5, February 1972, Pages 217–224, https://doi.org/10.1093/rheumatology/11.5.217 Published: 01 February 1972