gebhart/patrepeval huggingface dataset:
Enzymatic syntheses of alkyds. II: Lipase‐catalyzed polytransesterification of dichloroethyl fumarate with aliphatic and aromatic diols (1991)

fC\ll-trans" alkyds (unsaturated polyesters) were prepared by lipase-catalyzed polytransesterifications of diesters of fumaric acid and 1,4-butane diol. Industrially prepared alkyds, however, contain aromaticity in their backbone, which is introduced by phthalic acid derivatives and/or isomers. Thus, in this study, the specificity of lipases toward aromatic esters, diesters, and diols was investigated in order to find a means of introducing aromaticity in the lipase-catalyzed synthesis of "all-trans" alkyds. It was found that lipases from Pseudomonas and Mucor efficiently catalyzed the polytransesterification of dichloroethyl fumarate with aromatic diols such as benzene dimethanol. Crystallinity was found in the "alltrans" alkyds synthesized in acetonitrile. In order to determine whether this property was due to the nature of diol or the frequency of the double bond in the backbone of the polyester, other alkyds were prepared in which the length of the diol was changed as well as the number of carbons (odd or even) in the diol and the chemical nature of the diol (primary or secondary). In all cases crystallinity was found. It thus seems that the stereochemistry of the double bond is responsible for this property. Molecular weights, which were determined by gel permeation chromatography (GPC) analyses, were comparable with those of the industrially prepared alkyds.
Different concentrations of hepatocyte growth factor and fibroblast growth factor in the regulation of hepatic stem cells in rats (2010)

Objective To evaluate effect of different concentrations of hepatocyte growth factor (HGF) and fibroblast growth factor (FGF) on the regulation of hepatic stem cell proliferation.Methods Hepatic stem cells from rats were cultured under different conditions (Group A and B). HGF was used in Group A, with the concentrations of 1,5,10,20,40,80, and 100 ng/mL. Cytokines was not used in the control group. FGF was used in Group B, with the concentrations of 1,5,10,20,40,80, and 100 ng/mL. The optimal concentration for HGF or FGF was obtained by analyzing the dose-effect relationship in Groups A and B. Then hepatic stem cells were cultured in Group C, with the optimal concentration of HGF combined with 1,5,10,20,40,80, and 100 ng/mL FGF. Mononuclear cell direct cytotoxicity assay (MTT) was used to analyze the effect of HGF and FGF on hepatic stem cells proliferation.Results Different concentration of HGF and FGF could promote cell proliferation in a dose-dependent manner. The optimal concentration for HGF and FGF was 20 ng/mL and 10 ng/mL, respectively. Conclusion Both HGH and FGF can accelerate the proliferation of hepatic stem cells in vitro. There exists a synergistic action between them.
Regulation of Expression of Herpes Simplex Virus (HSV) Glycoprotein D in Vaccinia Recombinants Affects Their Ability to Protect from Cutaneous HSV-2 Disease (1989)

The effect of regulation of herpes simplex virus (HSV) type 1 glycoprotein D (gD-1) gene expression on HSV-specific immune response and protection from cutaneous HSV-2 disease was studied using vaccinia virus recombinants containing gD-1 under the control of early (VP176) or late (VP254) vaccinia virus promoters. Expression of gD-1 in VP176-infected cells was first observed at 2 h after infection. It did not depend on viral DNA replication. In VP254-infected cells, gD-1 was first observed at 24 h after infection and its expression depended on DNA replication. Immunized guinea pigs had similar titers of HSV-specific neutralizing antibody. However, HSV-specific T cell responses were significantly higher in VP176- than in VP254-immunized animals as determined by lymphoproliferation (P less than .005) and delayed type hypersensitivity (P less than .01). The reduced T cell responses of VP254-immunized guinea pigs correlated with poor gD-1 expression in VP254-infected antigen presenting cells (splenic adherent and epidermal cells). Immunization with VP176, but not with VP254, protected guinea pigs from primary (P less than .0005) and recurrent (P less than .0005) cutaneous HSV-2 lesions.
Plasma Protein β-2-Glycoprotein 1 Mediates Interaction between the Anti-tumor Monoclonal Antibody 3G4 and Anionic Phospholipids on Endothelial Cells (2006)

A promising target on tumor vasculature is phosphatidylserine (PS), an anionic phospholipid that resides exclusively on the inner leaflet of the plasma membrane of resting mammalian cells. We have shown previously that PS becomes exposed on the surface of endothelial cells (EC) in solid tumors. To target PS on tumor vasculature, the murine monoclonal antibody 3G4 was developed. 3G4 localizes to tumor vasculature, inhibits tumor growth, and enhances anti-tumor chemotherapies without toxicity in mice. A chimeric version of 3G4 is in clinical trials. In this study, we investigated the basis for the interaction between 3G4 and EC with surface-exposed PS. We demonstrate that antibody binding to PS is dependent on plasma protein beta-2-glycoprotein 1 (beta2GP1). beta2GP1 is a 50-kDa glycoprotein that binds weakly to anionic phospholipids under physiological conditions. We show that 3G4 enhances binding of beta2GP1 to EC induced to expose PS. We also show that divalent 3G4-beta2GP1 complexes are required for enhanced binding, since 3G4 Fab' fragments do not bind EC with exposed PS. Finally, we demonstrate that an artificial dimeric beta2GP1 construct binds to EC with exposed PS in the absence of 3G4, confirming that antibody binding is mediated by dimerization of beta2GP1. Together, these data indicate that 3G4 targets tumor EC by increasing the avidity of beta2GP1 for anionic phospholipids through formation of multivalent 3G4-beta2GP1 complexes.
An FPGA implementation of low-density parity-check code decoder with multi-rate capability (2005)

With superior error correction capability, low-density parity-check (LDPC) has initiated wide scale interests in wireless telecommunication fields. In the past, various structures of single code rate LDPC decoders have been implemented for different applications. However, in order to cover a wide range of service requirements and diverse interference conditions in wireless applications, LDPC decoders that can operate in both high and low code rates are desired. In this paper, a new multi-rate LDPC decoder architecture is presented and implemented in a Xilinx FPGA device. Through selection pins, three operating modes with the irregular 1/2 rate, regular 5/8 rate and regular 7/8 rate are supported. The measurement results show LDPC decoder can achieve BER below 10-5 at SNR of 1.4dB in the most critical case with the irregular 1/2 mode.
Problems in Management of Opportunistic Fungal Diseases (1989)

Opportunistic fungal infections are now common and will become more common as a consequence of the use of various regimens of immunosuppressive therapy for a variety of diseases and because of AIDS. These infections can be broadly divided into those that take advantage of a neutrophil defect (e.g., candidiasis, aspergillosis, and mucormycosis) and those that take advantage of a T cell mononuclear phagocyte defect (e.g., cryptococcosis, histoplasmosis, and coccidioidomycosis). Problems in diagnosis have been apparent since these infections were first recognized. The usual clinical specimens are often not revealing, and attempts to develop special serologic and biochemical tests have been made for years, but progress has been slow. Problems in treatment stem first from a lack of an early diagnosis and second from a lack of drugs that are effective and relatively nontoxic. It is difficult to evaluate new drugs if we cannot make accurate diagnoses; this difficulty is compounded by the low rate of autopsies in the academic hospitals where such studies are conducted.
Extrinsic neural control of nitric oxide synthase expression in the myenteric plexus of rat jejunum (1998)

1. The role of extrinsic innervation in the expression of nitric oxide synthase (NOS) in the myenteric plexus remains unknown. In this study, we investigated the effect of extrinsic denervation on NOS expression in rat jejunum. 2. NG-nitro-L-arginine methyl ester (L-NAME)-sensitive non-adrenergic, non-cholinergic (NANC) relaxations induced by transmural nerve stimulation were significantly increased in muscle strips obtained from rats treated with splanchnic ganglionectomy or 6-hydroxydopamine (6-OH-dopamine). Truncal vagotomy or treatment of the coeliac ganglia with capsaicin did not significantly affect NANC relaxations. 3. The number of NOS-immunopositive cells in the myenteric plexus was significantly increased in tissues obtained from rats treated with splanchnic ganglionectomy or 6-OH-dopamine. 4. Western blot analysis and Northern blot analysis showed a significant increase in the density of the immunoreactive NOS band and the NOS mRNA band, respectively, of the tissues obtained from rats treated with splanchnic ganglionectomy or 6-OH-dopamine. 5. Truncal vagotomy or treatment of the coeliac ganglia with capsaicin did not significantly affect the density of NOS band or NOS mRNA band. 6. It is concluded that neuronal NOS expression in the myenteric plexus is independent of vagus nerve and is negatively regulated by the splanchnic nerves in the rat small intestine.
Plasma Platelet-activating Factor Acetylhydrolase Deficiency in Japanese Patients with Asthma (1999)

Platelet-activating factor (PAF), a phospholipid with a wide range of proinflammatory actions, is immediately degraded and inactivated in vivo by PAF acetylhydrolase (PAF-AH). Surprisingly, 4% of the Japanese population lacks the extracellular isoform of this enzyme, plasma PAF-AH, due to a genetic missense (V279F) mutation. We studied the association of this mutation with asthma prevalence and phenotypes in the Japanese adult population. The allele frequency of V279F mutation was 18.6% in 279 patients with asthma (28.7% heterozygotes and 4.3% homozygotes) and 21.7% in 217 healthy subjects (32.3% heterozygotes and 5.5% homozygotes). V279F mutant allele prevalence was consistent regardless of asthma type (16.3% in atopic [n = 156] and 21.6% in nonatopic [n = 123]), or the severity of disease (21.7% in patients with mild [n = 97], 17.5% in those with moderate [n = 131], and 15.8% in those with severe [n = 51] asthma). Plasma PAF-AH activity was inversely proportional to the number of mutant alleles, and did not correlate with asthma prevalence, type, or severity. We concluded that plasma PAF-AH deficiency due to V279F mutation is not essential to the pathophysiology of asthma in the Japanese adult population.
Mass spectrometry-based metabolomics of single yeast cells (2013)

Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (ΔPFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering (2001)

Degradable and injectable hydrogels may be ideal for bone-tissue engineering, especially in the craniofacial region because of the ease of access for injection. Alginate hydrogels potentially could be used as injectable cell delivery vehicles, but they exhibit a limited range of mechanical properties and uncontrollable disintegration time. Therefore we synthesized new hydrogels, composed of poly(aldehyde guluronate) (PAG) and adipic acid dihydrazide, that have a wide range of mechanical stiffness and controllable degradation rate. MC3T3-E1 cells adhered and multiplied on PAG hydrogels in vitro. When primary rat calvarial osteoblasts were mixed with PAG hydrogels and subcutaneously injected into the backs of mice, mineralized bone tissues were formed 9 weeks following implantation. These hydrogels may find wide utility as an injectable delivery system for bone precursor cells as well as for other applications in tissue engineering.
Whole genome sequencing for lung cancer. (2012)

Lung cancer is a leading cause of cancer related morbidity and mortality globally, and carries a dismal prognosis. Improved understanding of the biology of cancer is required to improve patient outcomes. Next-generation sequencing (NGS) is a powerful tool for whole genome characterisation, enabling comprehensive examination of somatic mutations that drive oncogenesis. Most NGS methods are based on polymerase chain reaction (PCR) amplification of platform-specific DNA fragment libraries, which are then sequenced. These techniques are well suited to high-throughput sequencing and are able to detect the full spectrum of genomic changes present in cancer. However, they require considerable investments in time, laboratory infrastructure, computational analysis and bioinformatic support. Next-generation sequencing has been applied to studies of the whole genome, exome, transcriptome and epigenome, and is changing the paradigm of lung cancer research and patient care. The results of this new technology will transform current knowledge of oncogenic pathways and provide molecular targets of use in the diagnosis and treatment of cancer. Somatic mutations in lung cancer have already been identified by NGS, and large scale genomic studies are underway. Personalised treatment strategies will improve care for those likely to benefit from available therapies, while sparing others the expense and morbidity of futile intervention. Organisational, computational and bioinformatic challenges of NGS are driving technological advances as well as raising ethical issues relating to informed consent and data release. Differentiation between driver and passenger mutations requires careful interpretation of sequencing data. Challenges in the interpretation of results arise from the types of specimens used for DNA extraction, sample processing techniques and tumour content. Tumour heterogeneity can reduce power to detect mutations implicated in oncogenesis. Next-generation sequencing will facilitate investigation of the biological and clinical implications of such variation. These techniques can now be applied to single cells and free circulating DNA, and possibly in the future to DNA obtained from body fluids and from subpopulations of tumour. As costs reduce, and speed and processing accuracy increase, NGS technology will become increasingly accessible to researchers and clinicians, with the ultimate goal of improving the care of patients with lung cancer.
Professional Active Server Pages (1997)

From the Publisher:
Anybody and everybody who has responsibility for planning, building, or maintaining a web site -- on the Internet or a company intranet--you will find this book invaluable. It covers everything you need to know to move from traditional static HTML programming, to creating attractive and interactive pages that will make your site more productive -- and more fun.
Modification of orthogonal tRNAs: unexpected consequences for sense codon reassignment (2016)

Breaking the degeneracy of the genetic code via sense codon reassignment has emerged as a way to incorporate multiple copies of multiple non-canonical amino acids into a protein of interest. Here, we report the modification of a normally orthogonal tRNA by a host enzyme and show that this adventitious modification has a direct impact on the activity of the orthogonal tRNA in translation. We observed nearly equal decoding of both histidine codons, CAU and CAC, by an engineered orthogonal M. jannaschii tRNA with an AUG anticodon: tRNA<sup>Opt</sup> We suspected a modification of the tRNA<sup>Opt</sup><sub>AUG</sub> anticodon was responsible for the anomalous lack of codon discrimination and demonstrate that adenosine 34 of tRNA<sup>Opt</sup><sub>AUG</sub> is converted to inosine. We identified tRNA<sup>Opt</sup><sub>AUG</sub> anticodon loop variants that increase reassignment of the histidine CAU codon, decrease incorporation in response to the histidine CAC codon, and improve cell health and growth profiles. Recognizing tRNA modification as both a potential pitfall and avenue of directed alteration will be important as the field of genetic code engineering continues to infiltrate the genetic codes of diverse organisms.
Does Absolute Neutrophilia Predict Early Congestive Heart Failure After Acute Myocardial Infarction? A Cross-Sectional Study (2008)

The study shows that the presence of absolute neutrophilia during the first 12 hours after AMI can predict the occurrence of CHF. This association may help identify high-risk individuals, who might benefit from more aggressive interventions.
Separation of true fat and water images by correcting magnetic field inhomogeneity in situ. (1986)

Dixon's method of chemical shift imaging of a two-component system is modified and extended without requiring additional imaging time. The modified method allows one to obtain truly segregated fat and water images of animal tissues. This is accomplished by acquiring additional image data from which information about in situ magnetic field inhomogeneity and bulk magnetic susceptibility can be derived. Applications to various anatomic sections of the normal human body are illustrated. The method is compared with the standard Dixon technique of chemical shift image separation.
H-2 compatibility requirement for virus-specific T cell-mediated effector functions in vivo. I. Specificity of T cells conferring antiviral protection against lymphocytic choriomeningitis virus is associated with H-2K and H-2D. (1976)

Adoptive immunization of recipient mice preinfected with lymphocytic choriomeningitis virus (LCMV) is mediated exclusively by virus-specific thymus-derived lymphocytes, when assayed in a short-term transfer model. Protection, measured as reduction of LCMV plaque-forming units in spleens, is conferred only if donors of immune spleen cells and recipients share the K or the D region of the H-2 gene complex. I region compatibility is neither necessary nor sufficient. The F1 leads to Parent combination is as effective as a syngeneic system. Admixture of a 6-fold excess of immune allogeneic cells did not impair the protective effect exerted by syngeneic immune spleen cells in vivo. Furthermore, allogeneic spleen cells or target cells added in syngeneic systems in vitro did not allogeneicly inhibit or suppress cytolytic activity. H-2 mutant mice B6. H-2bf did not protect wild type H-2Kb B10.A(5r) or vice versa. Therefore, these mice define the gene(s) coding for the relevant cell-surface structure involved. These results are consistent with the idea that immune T cells, which are specific for virally altered cell-surface self structures impair virus growth in vivo either by lysing target cells, probably before infectious virus is released, or alternatively via activities exerted by lymphokines.
Effects of Antineoplastic Agents on Cytoplasmic and Membrane-Bound Heat Shock Protein 70 (Hsp70) Levels (2002)

Here we report on the study of the effects of different antineoplastic agents, including cytarabine, 4-hydroperoxyifosfamide, the activated form of ifosfamide, vincristine, and paclitaxel, with regard to their capacity to modulate the amount of cytoplasmic and membrane-bound heat shock protein 70 (Hsp70). Hsp70 levels were measured in the myelogenous leukemic cell line K562, in the human colon carcinoma cell line CX2, and in peripheral blood lymphocytes (PBL) under physiological conditions (37 degrees C), and following non-lethal heat shock at 41.8 degrees C. A concentration of 1 microM and an incubation period of 2 h were determined as non-lethal, since none of the different antineoplastic agents induced necrosis or apoptosis in untreated or heat-shocked cells under these conditions. Our results show that tubulin-interacting agents, including vincristine and paclitaxel, but not DNA-interacting agents, including cytarabine and ifosfamide, selectively increase the amount of cytoplasmic Hsp70 in tumor and normal cells, as measured by semi-quantitative Western blot analysis. Mechanistically, a vincristine- and paclitaxel-induced tubulin assembly, as demonstrated by immunofluorescence microscopy, might be responsible for the elevated cytoplasmic Hsp70 levels. Interestingly, an increased membrane expression of Hsp70 following treatment with vincristine or paclitaxel was selectively observed on tumor cells, but not on normal cells.
Über die Si_N‐Bindung, XXVI Darstellung und Reaktionen von 1.2‐Bis‐trimethylsilyliminen (1968)

Abstract Aus Benzil und Phenanthrenchinon werden mit Natrium‐bis‐trimethylsilyl‐amid die entsprechenden Bis‐trimethylsilylimine ( 1 , 2 ) gewonnen. Die Alkoholyse der 1.2‐Bis‐trimethylsilylimine führt zu den bisher unbekannten 1.2‐Diiminen, ihre Umsetzung mit Säurechloriden oder kovalenten Halogeniden zu den N . N ′‐Diacylderivaten der Diimine und zu einer Reihe heterocyclischer Systeme.
Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus (1997)

Three Aedes albopictus (mosquito) cell lines persistently infected with Sindbis virus excluded the replication of both homologous (various strains of Sindbis) and heterologous (Aura, Semliki Forest, and Ross River) alphaviruses. In contrast, an unrelated flavivirus, yellow fever virus, replicated equally well in uninfected and persistently infected cells of each line. Sindbis virus and Semliki Forest virus are among the most distantly related alphaviruses, and our results thus indicate that mosquito cells persistently infected with Sindbis virus are broadly able to exclude other alphaviruses but that exclusion is restricted to members of the alphavirus genus. Superinfection exclusion occurred to the same extent in three biologically distinct cell clones, indicating that the expression of superinfection exclusion is conserved among A. albopictus cell types. Superinfection of persistently infected C7-10 cells, which show a severe cytopathic effect during primary Sindbis virus infection, by homologous virus does not produce cytopathology, consistent with the idea that cytopathology requires significant levels of viral replication. A possible model for the molecular basis of superinfection exclusion, which suggests a central role for the alphavirus trans-acting protease that processes the nonstructural proteins, is discussed in light of these results.
Surface Plasmon-Mediated Energy Transfer in Heterogap Au−Ag Nanowires (2008)

We report the observation of energy transfer from a gold (Au) nanodisk pair to a silver (Ag) nanowire across a 120 nm gap via surface plasmon resonance (SPR) excitation. The enhanced electromagnetic (EM) fields generated by Au SPR excitation induce oscillation of the conduction electrons in the Ag segment, transferring energy to it even though the Ag segment has only weak resonant interactions with the incident EM radiation. The induced Ag SPR produces strong EM fields at the position of the Ag segment, leading to a Raman signal approximately 15 times greater than when the Ag segment is alone (not adjacent to the Au nanodisk pair). The Raman intensity is found to depend nonlinearly on the incident laser intensity for laser power densities of 10 kW/cm(2), which is consistent with the results of EM theory calculations which are not able to account for the factor of 15 enhancement based on a linear mechanism. This suggests that energy transfer from the Au disk pair to the Ag segment involves an enhanced nonlinear polarization mechanism such as can be produced by the electronic Kerr effect or stimulated Raman scattering.
F2RL3methylation in blood DNA is a strong predictor of mortality (2014)

F2RL3 methylation is a strong predictor of mortality, including all-cause, cardiovascular, cancer and other mortality. Systemic adverse effects of smoking may be mediated by pathways associated with F2RL3 methylation.