gebhart/patrepeval huggingface dataset:
Synthesis of α-(p-Aminophenyl)- and α-(p-Chlorophenyl)-β-arylpropionitriles by Catalytic Reduction of Stilbenenitriles (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of α-(p-Aminophenyl)- and α-(p-Chlorophenyl)-β-arylpropionitriles by Catalytic Reduction of StilbenenitrilesGordon N. WalkerCite this: J. Med. Chem. 1965, 8, 5, 583–588Publication Date (Print):September 1, 1965Publication History Published online1 May 2002Published inissue 1 September 1965https://pubs.acs.org/doi/10.1021/jm00329a008https://doi.org/10.1021/jm00329a008research-articleACS PublicationsRequest reuse permissionsArticle Views150Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Versatile Switching in Substrate Topicity: Supramolecular Chirality Induction in Di‐ and Trinuclear Host Complexes (2012)

Supramolecular chirality effects have been achieved both for ditopic and monotopic substrates by using a programmable bis-salphen scaffold that incorporates either two or three Zn nuclei. The dinuclear host shows preferential chirogenesis in the presence of ditopic systems, whereas effective chirality transfer to the trinuclear complex is realized through monotopic binding. The mode of binding in the trinuclear host has been investigated through X-ray crystallography, CD measurements, UV/Vis spectroscopy, and DFT analysis. The bis-salphen scaffold holds promise for the development of substrate-specific host systems useful for determination of the absolute configuration of various types of organic molecules.
Jet-Pylon Interaction of High Bypass Ratio Separate Flow Nozzle Configurations (2004)

NASA Langley Research Center, Hampton, Virginia, 23681-0001 USA An experimental investigation was performed of the acoustic effects of jet-pylon interaction for separate flow and chevron nozzles of both bypass ratio five and eight. The models corresponded to an approximate scale factor of nine. Cycle conditions from approach to takeoff were tested at wind tunnel free jet Mach numbers of 0.1, 0.2 and 0.28. An eight-chevron core nozzle, a sixteen chevron fan nozzle, and a pylon were primary configuration variables. In addition, two orientations of the chevrons relative to each other and to the pylon were tested. The effect of the pylon on the azimuthal directivity was investigated for the baseline nozzles and the chevron nozzles. For the bypass ratio five configuration, the addition of the pylon reduces the noise by approximately 1 EPNdB compared to the baseline case and there is little effect of azimuthal angle. The core chevron produced a 1.8 EPNdB reduction compared to the baseline nozzle. Adding a pylon to the chevron core nozzle produces an effect that depends on the orientation of the chevron relative to the pylon. The azimuthal directivity variation remains low at less than 0.5 EPNdB. For the bypass ratio eight configuration the effect of adding a pylon to the baseline nozzle is to slightly increase the noise at higher cycle points and for the case with a core chevron the pylon has little additional effect. The azimuthal angle effect continues to be very small for the bypass ratio eight configurations. A general impact of the pylon was observed for both fan and core chevrons at both bypass ratios. The pylon reduces the typical low frequency benefit of the chevrons, even eliminating it in some cases, while not impacting the high frequency. On an equal ideal thrust basis, the bypass ratio eight baseline nozzle was about 5 EPNdB lower than the bypass ratio five baseline nozzle at the highest cycle condition, however, with a pylon installed the difference decreased to about 4 EPNdB.
Enhancement of Muscle Gene Delivery with Pseudotyped Adeno-Associated Virus Type 5 Correlates with Myoblast Differentiation (2001)

Adeno-associated virus (AAV)-based muscle gene therapy has achieved tremendous success in numerous animal models of human diseases. Recent clinical trials with this vector have also demonstrated great promise. However, to achieve therapeutic benefit in patients, large inocula of virus will likely be necessary to establish the required level of transgene expression. For these reasons, efforts aimed at increasing the efficacy of AAV-mediated gene delivery to muscle have the potential for improving the safety and therapeutic benefit in clinical trials. In the present study, we compared the efficiency of gene delivery to mouse muscle cells for recombinant AAV type 2 (rAAV-2) and rAAV-2cap5 (AAV-2 genomes pseudo-packaged into AAV-5 capsids). Despite similar levels of transduction by these two vectors in undifferentiated myoblasts, pseudotyped rAAV-2cap5 demonstrated dramatically enhanced transduction in differentiated myocytes in vitro (>500-fold) and in skeletal muscle in vivo (>200-fold) compared to rAAV-2. Serotype-specific differences in transduction efficiency did not directly correlate with viral binding to muscle cells but rather appeared to involve endocytic or intracellular barriers to infection. Furthermore, application of this pseudotyped virus in a mouse model of Duchenne's muscular dystrophy also demonstrated significantly improved transduction efficiency. These findings should have a significant impact on improving rAAV-mediated gene therapy in muscle.
The amazing Internet challenge: how leading projects use library skills to organize the Web (2000)

From the Publisher:
The Amazing Internet Challenge can help you organize web resources for your users. Insiders from twelve digital libraries, such as Agriculture Network Information Center (AgNIC), Infomine, Internet Public Library, and Social Science Information Gateway (SOSIG), reveal their selection criteria, evaluation process, funding sources and project budgets, and software and hardware tools.
Toxic Trypsin Digest Fragment from the Bacillus thuringiensis Parasporal Protein (1987)

Enzymatic digestion in vitro of the Bacillus thuringiensis protoxin presumably releases and activates the toxin in a manner analogous to that which occurs when a B. thuringiensis sporulated fermentation preparation passes through the midgut of a lepidopteran larva. Therefore, a sporulated culture of B. thuringiensis subsp. kurstaki (serotype 3a3b) HD-263 was treated with trypsin to release an activated toxin soluble in bicarbonate buffer. A 63-kilodalton protein, toxic to cabbage looper larvae (Trichoplusia ni) and to lepidopteran cells in culture, was purified to homogeneity from this trypsin digest. The larvicide, a glycoprotein containing 5% carbohydrate (wt/wt), was purified from the soluble B. thuringiensis trypsin digest by using ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic-interaction chromatography. Its amino acid composition was high in nonpolar residues and unusually low in lysine and histidine. The isoelectric point was 6.5, and the amino acid on the N terminus was identified as isoleucine. The toxin was only slightly soluble in aqueous buffers unless the chaotropic agent potassium thiocyanate was added. Partial characterization of the toxin indicated that it corresponds well with reported sequences deduced from cloned genes.
Cutting edge: constitutive B cell receptor signaling is critical for basal growth of B lymphoma (2006)

In Results, in the heading of the third to last paragraph and in the last sentence of the same paragraph, the word "central" is incorrect. The corrected heading and sentence are shown below.
Microarray Analysis of Murine Retinal Light Damage Reveals Changes in Iron Regulatory, Complement, and Antioxidant Genes in the Neurosensory Retina and Isolated RPE (2012)

The concept of a photooxidative stress-induced vicious cycle of increased iron uptake leading to further oxidative stress was introduced.
Research into fuzzy extensions of information retrieval (1986)

Modern computerized information retrieval systems consist of mechanisms to acquire, describe (e.g., index), and store "documents", and to receive, analyze, and respond to queries for information for users. A key element is the index language, by which the users (or user intermediaries) and indexers can communicate. Modern technology allows natural language processing mechanisms to begin to be incorporated in the sense of matching terms found in the free text specification of the query and the free text within the document.
Calcium Ion Adsorption on Phospholipid Bilayers Theoretical Interpretation (1994)

A theoretical approach to phase transition enthalpy change as a function of calcium ion concentration was studied in terms of calcium ion adsorption on a phospholipid surface. The phospholipids used in this study were dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylethanolamine (DPPE), and dipalmitoylphosphatidylglycerol (DPPG). The theory used here is based on the DLVO theory, taking into account electrostatic interaction caused by calcium ion adsorption on the membrane surface. Calcium ion effects on DPPC and DPPG phase behavior can be explained by Helmholtz free energy change. Experimental enthalpy change is dependent on the electrical contribution in the free energy equation. This contribution is related to the binding constant K and effective surface area a'. Binding parameters, binding constant K and effective surface area a' were K=100 M-1 and a'=60, 000 m2/g for DPPG, and K=40 M-1 and a'=750 m2/g for DPPC as optimal values. These values for DPPE could not be determined owing to the absence of enthalpy change.
Biomedical Science, Engineering and Technology (2012)

This innovative book integrates the disciplines of biomedical science, biomedical engineering, biotechnology, physiological engineering, and hospital management technology. Herein, Biomedical science covers topics on disease pathways, models and treatment mechanisms, and the roles of red palm oil and phytomedicinal plants in reducing HIV and diabetes complications by enhancing antioxidant activity. Biomedical engineering coves topics of biomaterials (biodegradable polymers and magnetic nanomaterials), coronary stents, contact lenses, modelling of flows through tubes of varying cross-section, heart rate variability analysis of diabetic neuropathy, and EEG analysis in brain function assessment. Biotechnology covers the topics of hydrophobic interaction chromatography, protein scaffolds engineering, liposomes for construction of vaccines, induced pluripotent stem cells to fix genetic diseases by regenerative approaches, polymeric drug conjugates for improving the efficacy of anticancer drugs, and genetic modification of animals for agricultural use. Physiological engineering deals with mathematical modelling of physiological (cardiac, lung ventilation, glucose regulation) systems and formulation of indices for medical assessment (such as cardiac contractility, lung disease status, and diabetes risk). Finally, Hospital management science and technology involves the application of both biomedical engineering and industrial engineering for cost-effective operation of a hospital.
Directed Self-Assembly of Monodisperse Phospholipid Bilayer Nanodiscs with Controlled Size (2004)

Using a recently described self-assembly process (Bayburt, T. H.; Grinkova, Y. V.; Sligar, S. G. Nano Letters 2002, 2, 853-856), we prepared soluble monodisperse discoidal lipid/protein particles with controlled size and composition, termed Nanodiscs, in which the fragment of dipalmitoylphosphatidylcholine (DPPC) bilayer is surrounded by a helical protein belt. We have customized the size of these particles by changing the length of the amphipathic helical part of this belt, termed membrane scaffold protein (MSP). Herein we describe the design of extended and truncated MSPs, the optimization of self-assembly for each of these proteins, and the structure and composition of the resulting Nanodiscs. We show that the length of the protein helix surrounding the lipid part of a Nanodisc determines the particle diameter, as measured by HPLC and small-angle X-ray scattering (SAXS). Using different scaffold proteins, we obtained Nanodiscs with the average size from 9.5 to 12.8 nm with a very narrow size distribution (+/-3%). Functionalization of the N-terminus of the scaffold protein does not perturb their ability to form homogeneous discoidal structures. Detailed analysis of the solution scattering confirms the presence of a lipid bilayer of 5.5 nm thickness in Nanodiscs of different sizes. The results of this study provide an important structural characterization of self-assembled phospholipid bilayers and establish a framework for the design of soluble amphiphilic nanoparticles of controlled size.
A microfluidic device for separating motile sperm from nonmotile sperm via inter-streamline crossings (2003)

In vitro fertilization using small amounts of cryopreserved sperm would benefit from efficient microscale methods to sort "healthy" motile sperm from nonmotile ones. This paper describes a microfluidic system with embedded reservoirs that sorts sperm based on the ability of motile sperm to move out of their initial streamlines in a laminar fluid stream. The device has horizontally oriented sample inlet and outlet reservoirs with different relative heights that serve the dual purpose of also being a pumping system that pumps fluids by hydrostatic pressure and surface tension. Sperm sorting is necessary for cryopreservation and intracytoplasmic sperm injection of sperm from men with low viable sperm counts. Since they have few viable sperm, it is difficult to obtain the sperm in an efficient manner using current procedures.
Obstacles and pitfalls in the PEGylation of therapeutic proteins. (2008)

In recent years, PEGylation has become widely used as a post-production modification methodology for improving the biomedical efficacy and physicochemical properties of therapeutic proteins. Several marketed drugs that have already been in use for more than a decade have proved the applicability and safety of this technology and, with the successes already achieved, it is expected that PEGylation will be applied to other potential therapeutic proteins. The non-biodegradable nature of PEG, however, may become a limiting factor for the next generation of protein pharmaceuticals, for which use in high concentrations and in the long term is the aim, especially considering the trend for the use of branched and high molecular mass PEGs. This review addresses various obstacles and pitfalls in the production of PEGylated biopharmaceuticals, in particular, the specificity of PEGylation reactions, separation and purification issues, the analysis of inherently polydisperse PEG reagents and PEGylated products, the consistency of products and processes, and the accurate determination of pharmacokinetic properties.
Towards foveated rendering for gaze-tracked virtual reality (2016)

Foveated rendering synthesizes images with progressively less detail outside the eye fixation region, potentially unlocking significant speedups for wide field-of-view displays, such as head mounted displays, where target framerate and resolution is increasing faster than the performance of traditional real-time renderers. To study and improve potential gains, we designed a foveated rendering user study to evaluate the perceptual abilities of human peripheral vision when viewing today's displays. We determined that filtering peripheral regions reduces contrast, inducing a sense of tunnel vision. When applying a postprocess contrast enhancement, subjects tolerated up to 2× larger blur radius before detecting differences from a non-foveated ground truth. After verifying these insights on both desktop and head mounted displays augmented with high-speed gaze-tracking, we designed a perceptual target image to strive for when engineering a production foveated renderer. Given our perceptual target, we designed a practical foveated rendering system that reduces number of shades by up to 70% and allows coarsened shading up to 30° closer to the fovea than Guenter et al. [2012] without introducing perceivable aliasing or blur. We filter both pre- and post-shading to address aliasing from undersampling in the periphery, introduce a novel multiresolution- and saccade-aware temporal antialising algorithm, and use contrast enhancement to help recover peripheral details that are resolvable by our eye but degraded by filtering. We validate our system by performing another user study. Frequency analysis shows our system closely matches our perceptual target. Measurements of temporal stability show we obtain quality similar to temporally filtered non-foveated renderings.
Interferon gamma receptor deficient mice are resistant to endotoxic shock. (1994)

Antibody neutralization studies have established interferon gamma (IFN-gamma) as a critical mediator of endotoxic shock. The advent of IFN-gamma receptor negative (IFN gamma R-/-) mutant mice has enabled a more direct assessment of the role of IFN-gamma in endotoxin (lipopolysaccharide [LPS]-induced shock. We report that IFN gamma R-/- mice have an increased resistance to LPS-induced toxicity, this resistance manifesting well before the synthesis and release of LPS-induced IFN-gamma. LPS-induced lymphopenia, thrombocytopenia, and weight loss seen in wild-type mice were attenuated in IFN gamma R-/- mice. IFN gamma R-/- mice tolerated 100-1,000 times more LPS than the minimum lethal dose for wild-type mice in a D-galactosamine (D-GalN)/LPS model. Serum tumor necrosis factor (TNF) levels were 10-fold reduced in mutant mice given LPS or LPS/D-GalN. Bone marrow and splenic macrophages from IFN gamma R-/- mice had a four- to sixfold decreased LPS-binding capacity which correlated with similar reduction in CD14. Serum from mutant mice reduced macrophage LPS binding by a further 50%, although LPS binding protein was only 10% reduced. The expression of TNF receptor I (p55) and II (p75) was identical between wild-type and mutant mice. Thus, depressed TNF synthesis, diminished expression of CD14, and low plasma LPS-binding capacity, in addition to blocked IFN-gamma signaling in the mutant mice, likely to combine to manifest in the resistant phenotype of IFN gamma R-/- mice to endotoxin.
The quest for the SPIN transistor (2001)

Microelectronics researchers have been investigating spin for at least 20 years. Indeed, their discoveries revolutionized hard-disk drives, which since 1998 have used a spin-based phenomenon to cram more bits than ever on to their disks. Within three years, Motorola Inc. and IBM Corp. are expected to take the next step, introducing the first commercial semiconductor chips to exploit spin-a new form of random access memory called M (for magnetic) RAM. In this paper, the author describes how the mystical property of electron spin is revolutionizing the memory business. If it can do the same with logic, electronics will become "spintronics".
A computational framework for simulating and analyzing human and animal movement (2000)

Computer simulations provide a framework for exploring the biomechanics of movement. The authors have developed a software system that lets users create computer graphics simulations of human and animal movement. This software provides a platform on which the biomechanics community can build a library of simulations that can be exchanged, tested and improved through multi-institutional collaboration.
Quad-tree segmentation for texture-based image query (1994)

In this paper we propose a technique for segmenting images by texture content with application to indexing images in a large image database. Using quad-tree decomposition, texture features are extracted from spatial blocks at a hierarchy of scales in each image. The quad-tree is grown by iteratively testing conditions for splitting parent blocks based on texture content of children blocks. While this approach does not achieve smooth identification of texture region borders, homogeneous blocks of texture are extracted which can be used in a database index. Furthermore, this technique performs the segmentation directly using image spatial-frequency data. In the segmentation reported here, texture features are extracted from the wavelet representation of the image. This method however, can use other subband decompositions including Discrete Cosine Transform (DCT), which has been adopted by the JPEG standard for image coding. This makes our segmentation method extremely applicable to databases containing compressed image data. We show application of the texture segmentation towards providing a new method for searching for images in large image databases using “Query-by-texture.”
LRRK2 phosphorylates moesin at threonine-558: characterization of how Parkinson's disease mutants affect kinase activity (2007)

Mutations in the LRRK2 (leucine-rich repeat kinase-2) gene cause late-onset PD (Parkinson's disease). LRRK2 contains leucine-rich repeats, a GTPase domain, a COR [C-terminal of Roc (Ras of complex)] domain, a kinase and a WD40 (Trp-Asp 40) motif. Little is known about how LRRK2 is regulated, what its physiological substrates are or how mutations affect LRRK2 function. Thus far LRRK2 activity has only been assessed by autophosphorylation and phosphorylation of MBP (myelin basic protein), which is catalysed rather slowly. We undertook a KESTREL (kinase substrate tracking and elucidation) screen in rat brain extracts to identify proteins that were phosphorylated by an activated PD mutant of LRRK2 (G2019S). This led to the discovery that moesin, a protein which anchors the actin cytoskeleton to the plasma membrane, is efficiently phosphorylated by LRRK2, at Thr558, a previously identified in-vivo-phosphorylation site that regulates the ability of moesin to bind actin. LRRK2 also phosphorylated ezrin and radixin, which are related to moesin, at the residue equivalent to Thr558, as well as a peptide (LRRKtide: RLGRDKYKTLRQIRQ) encompassing Thr558. We exploited these findings to determine how nine previously reported PD mutations of LRRK2 affected kinase activity. Only one of the mutations analysed, namely G2019S, stimulated kinase activity. Four mutations inhibited LRRK2 kinase activity (R1941H, I2012T, I2020T and G2385R), whereas the remainder (R1441C, R1441G, Y1699C and T2356I) did not influence activity. Therefore the manner in which LRRK2 mutations induce PD is more complex than previously imagined and is not only caused by an increase in LRRK2 kinase activity. Finally, we show that the minimum catalytically active fragment of LRRK2 requires an intact GTPase, COR and kinase domain, as well as a WD40 motif and a C-terminal tail. The results of the present study suggest that moesin, ezrin and radixin may be LRRK2 substrates, findings that have been exploited to develop the first robust quantitative assay to measure LRRK2 kinase activity.