gebhart/patrepeval huggingface dataset:
Akt/Protein Kinase B Prevents Injury-Induced Motoneuron Death and Accelerates Axonal Regeneration (2000)

Motoneurons require neurotrophic factors for their survival and axonal projection during development, as well as nerve regeneration. By using the axotomy-induced neuronal death paradigm and adenovirus-mediated gene transfer, we attempted to gain insight into the functional significances of major growth factor receptor downstream cascades, Ras-extracellular signal-regulated kinase (Ras-ERK) pathway and phosphatidylinositol-3 kinase-Akt (PI3K-Akt) pathway. After neonatal hypoglossal nerve transection, the constitutively active Akt-overexpressing neurons could survive as well as those overexpressing Bcl-2, whereas the constitutively active ERK kinase (MEK)-overexpressing ones failed to survive. A dominant negative Akt experiment demonstrated that inhibition of Akt pathway hastened axotomy-induced neuronal death in the neonate. In addition, the dominant active Akt-overexpressing adult hypoglossal neurons showed accelerated axonal regeneration after axotomy. These results suggest that Akt plays dual roles in motoneuronal survival and nerve regeneration in vivo and that PI3K-Akt pathway is probably more vital in neuronal survival after injury than Ras-ERK pathway.
Hypertensive cardiomyopathy. Myocyte nuclei hyperplasia in the mammalian rat heart. (1990)

To determine whether long-term hypertension leads to hyperplasia of myocyte nuclei in the heart, a phenomenon suspected to occur in humans, renal hypertension was produced in rats and the animals were killed 8 mo later. Arterial blood pressure remained elevated for approximately 5 mo, but decreased progressively in the last 3 mo so that at 8 mo this parameter was practically identical to that found in controls. Moreover, left ventricular end diastolic pressure was markedly increased in experimental animals in association with a substantial decrease in left ventricular dP/dt. The alteration of these physiological measurements was indicative of severe ventricular dysfunction. Quantitative analysis of the transmural distribution of myocyte nuclei in the left ventricle showed 36 and 23% increases in myocyte nuclei concentration in the epimyocardium and endomyocardium, respectively. These changes in nuclei were accompanied by 25 and 16% reductions in myocyte cell volume per nucleus in the outer and inner layers of the wall. In conclusion, long-term hypertension leads to impairment of ventricular function and proliferation of nuclei in myocytes.
Increased expression of the pro-apoptotic ATP-sensitive P2X7 receptor in experimental and human glomerulonephritis (2006)

Although there is an association between expression of the pro-inflammatory and pro-apoptotic P2X(7) receptor and glomerulonephritis in these rodent models, and in at least one form of human glomerulonephritis, the underlying relationship and its functional significance remain to be explored.
A LISP‐based image analysis system with applications to microscopy (1987)

SUMMARY LISPIX is an image processing and analysis system consisting of a sub‐system of image processing commands implemented in FORTRAN and a collection of analysis algorithms implemented in LISP. Examples are given of using the system for microanalysis applications. The processing commands are useful alone for simple tasks. For analysis applications, the LISP programming environment, for which the processing commands are designed, allows rapid development of algorithms and a convenient way to use them. Appendices contain definitions of the most useful commands as well as examples of the LISP algorithms.
Energetics of Biological Nitrogen Fixation: Determination of the Ratio of Formation of H2 to NH4+ Catalysed by Nitrogenase of Klebsiella pneumoniae in vivo (1977)

Nitrogen fixation (Nif)-derepressed mutants of Klebsiella pneumoniae consumed, under optimum conditions, 7.5 to 8.5 mol glucose per mol N2 fixed. The nitrogenase system of these mutants catalysed the production of about 1.3 mol H2 per mol N2 reduced. Almost one-third of the energy as ATP and reductant used by nitrogenase in vivo may be lost in H2 production, since an ATP/2e ratio of approximately 4 was obtained. Nitrogenase-catalysed H2 production was not substantially suppressed by increasing the partial pressure of N2 from 0.2 atm (20 kPa) to 1 atm (101 kPa). In the absence of N2, H2 production catalysed by nitrogenase increased about threefold. It is concluded that nitrogenase-catalysed H2 production is of major importance in the overall efficiency of biological N2 fixation in vivo.
Essential Fatty Acids/Eicosanoid Biosynthesis in the Skin: Biological Significance (1994)

The first indication that dietary fat may be essential for healthy growing animals was presented in 1918 by Aron, who proposed that butter has a nutrient value that cannot be provided by other dietary components . This report suggested that there was a special nutritive value inherent in fat apart from its caloric contribution and that this possibly was related to the presence of certain lipids. In 1929, Burr and Burr presented the first in a series of papers outlining a “new deficiency disease produced by the rigid exclusion of fat from the diet.” They developed the hypothesis that warm-blooded animals in general cannot synthesize appreciable quantities of certain fatty acids. In 1930, both investigators significantly added to their earlier work by presenting evidence that the dietary inclusion of linoleic acid alone could reverse all deficiency symptoms resulting from a fat-free diet and thus linoleic acid (LA or 18:2n-6) was heralded as an “essential fatty acid” (EFA) . This pioneering study contained additional observations which indicated that besides the visible scaliness of the skin, animals with essential fatty acid deficiency (EFA-deficiency) also experience increased water consumption without increased urine output leading to speculation of increased water loss through the skin. Thus, Burr recognized in these early studies the two major defects that have been associated with EFA deficiency in cutaneous biology, namely: epidermal hyperproliferation and an increased permeability of the skin to water. Three major families of unsaturated fatty acids (UFAs) are characteristic of mammalian species: the n-9, the n-6, and the n-3 UFAs. The n-6 and n-3 polyunsaturated fatty acids (PUFAs) are defined by the position of the double bond closest to the terminal methyl group of the fatty acid molecule.
Solar cell efficiency tables (version 49) (2016)

Abstract Consolidated tables showing an extensive listing of the highest independently confirmed efficiencies for solar cells and modules are presented. Guidelines for inclusion of results into these tables are outlined, and new entries since June 2016 are reviewed. Copyright © 2016 John Wiley &amp; Sons, Ltd.
P‐5: A‐Si TFT Integrated Gate Driver with AC‐driven Single Pull‐Down Structure (2006)

Abstract A novel gate driver circuit using a‐Si TFT has been developed. It has AC driven single pull‐down structure for maintaining gate line voltage. Degradation of the circuit is retarded because of relieved stress to the pull‐down TFT by alternating the gate node voltage. The circuit has been successfully integrated in 14.1‐in. XGA TFT‐LCD panel.
ELF-2, a New Member of the Eph Ligand Family, Is Segmentally Expressed in Mouse Embryos in the Region of the Hindbrain and Newly Forming Somites (1995)

The Eph receptors are the largest known family of receptor tyrosine kinases and are notable for distinctive expression patterns in the nervous system and in early vertebrate development. However, all were identified as orphan receptors, and only recently have there been descriptions of a corresponding family of ligands. We describe here a new member of the Eph ligand family, designated ELF-2 (Eph ligand family 2). The cDNA sequence for mouse ELF-2 indicates that it is a transmembrane ligand. It shows closest homology to the other known transmembrane ligand in the family, ELK-L/LERK-2/Cek5-L, with 57% identity in the extracellular domain. There is also striking homology in the cytoplasmic domain, including complete identity of the last 33 amino acids, suggesting intracellular interactions. On cell surfaces, and in a cell-free system, ELF-2 binds to three closely related Eph family receptors, Elk, Cek10 (apparent ortholog of Sek-4 and HEK2), and Cek5 (apparent ortholog of Nuk/Sek-3), all with dissociation constants of approximately 1 nM. In situ hybridization of mouse embryos shows ELF-2 RNA expression in a segmental pattern in the hindbrain region and the segmenting mesoderm. Comparable patterns have been described for Eph family receptors, including Sek-4 and Nuk/Sek-3, suggesting roles for ELF-2 in patterning these regions of the embryo.
A novel assay for replicative lifespan in (2004)

The replicative lifespan of Saccharomyces cerevisiae is determined by both genetic and environmental factors. Many of the same factors determine the lifespan of metazoan animals. The lack of fast and reliable lifespan assays has limited the pace of yeast aging research. In this study we describe a novel strategy for assaying replicative lifespan in yeast, and apply it in a screening of mutants that are resistant to pro-oxidants. The assay reproduces the lifespan-shortening effects of deleting SIR2 and of growth in the presence of paraquat, a pro-oxidant. The lifespan-increasing activity of resveratrol is also reproduced. Compared to current assays, this new strategy promises to significantly increase the possible number of replicative-lifespan determinations.
Synthesis of oligothymidylates and nucleoside cyclic phosphates by oxidation-reduction condensation (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of oligothymidylates and nucleoside cyclic phosphates by oxidation-reduction condensationTeruaki Mukaiyama and Mitsunori HashimotoCite this: J. Am. Chem. Soc. 1972, 94, 24, 8528–8532Publication Date (Print):November 1, 1972Publication History Published online1 May 2002Published inissue 1 November 1972https://pubs.acs.org/doi/10.1021/ja00779a039https://doi.org/10.1021/ja00779a039research-articleACS PublicationsRequest reuse permissionsArticle Views700Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The chondroitin sulfate proteoglycan NG2 is a tumour-specific antigen on the chemically induced rat chondrosarcoma HSN (1994)

N-terminal sequences of 19 and 11 amino acids obtained from 2 different tryptic fragments of the tumour-specific antigen on the chemically induced rat chondrosarcoma HSN show a 100% homology with the rat chondroitin sulfate proteoglycan NG2. Using a scheme of overlapping oligonucleotide primers we have cloned by PCR amplification the cDNA for the specific antigen of the HSN tumour that is immunogenic in immunocompetent CBH/Cbi rats and now report that its cDNA sequence is identical to that of NG2. The cDNA codes for a transmembrane protein of 2,325 amino acids with a large extracellular domain of 2,224 amino acids containing 2 cysteine-rich regions, a transmembrane domain (25 amino acids) and a short cytoplasmic tail (76 amino acids). The tumour-specific determinant was found to lie between amino acid residues 556 and 992 on the core glycoprotein.
The Vacuolar Targeting Signal of the 2S Albumin from Brazil Nut Resides at the C Terminus and Involves the C-Terminal Propeptide as an Essential Element (1996)

Genetic constructs in which different N- and C-terminal segments of Brazil nut (Bertholletia excelsa H.B.K.) 2S albumin were fused to secretory yeast invertase were transformed into tobacco (Nicotiana tabacum) plants to investigate the vacuolar targeting signal of the 2S albumin. None of the N-terminal segments, including the complete precursor containing all propeptides, was able to direct the invertase to the vacuoles. However, a short C-terminal segment comprising the last 20 amino acids of the precursor was sufficient for efficient targeting of yeast invertase to the vacuoles of the transformed tobacco plants. Further analyses showed that peptides of 16 and 13 amino acids of the C-terminal segment were still sufficient, although they had slightly lower efficiency. When segments of 9 amino acids or shorter were analyzed, a decrease to approximately 30% was observed. These segments included the C-terminal propeptide of four amino acids (Ile-Ala-Gly-Phe). When the 2S albumin was expressed in tobacco, it was also localized to the vacuoles of mesophyll cells. If the C-terminal propeptide was deleted from the 2S albumin precursor, all of this truncated 2S albumin was secreted from the tobacco cells. These results indicate that the C-terminal propeptide is necessary but not sufficient for vacuolar targeting. In addition, an adjacent segment of at least 12 amino acids of the mature protein is needed to form the complete signal for efficient targeting.
Enzymatic Synthesis of Glycosaminoglycan Heparin (2007)

Heparin and its low molecular weight heparin derivatives, widely used as clinical anticoagulants, are acidic polysaccharide members of a family of biomacromolecules called glycosaminoglycans (GAGs). Heparin and the related heparan sulfate are biosynthesized in the Golgi apparatus of eukaryotic cells. Heparin is a polycomponent drug that currently is prepared for clinical use by extraction from animal tissues. A heparin pentasaccharide, fondaparinux, has also been prepared through chemical synthesis for use as a homogenous anticoagulant drug. Recent enabling technologies suggest that it may now be possible to synthesize heparin and its derivatives enzymatically. Moreover, new technologies including advances in synthetic carbohydrate synthesis, enzyme-based GAG synthesis, micro- and nano-display of GAGs, rapid on-line structural analysis, and microarray/microfluidic technologies might be applied to the enzymatic synthesis of heparins with defined structures and exhibiting selected activities. The advent of these new technologies also makes it possible to consider the construction of an artificial Golgi to increase our understanding of the cellular control of GAG biosyntheses in this organelle.
AFilter: adaptable XML filtering with prefix-caching suffix-clustering (2006)

XML message filtering problem involves searching for instances of a given, potentially large, set of patterns in a continuous stream of XML messages. Since the messages arrive continuously, it is essential that the filtering rate matches the data arrival rate. Therefore, the given set of filter patterns needs to be indexed appropriately to enable real-time processing of the streaming XML data. In this paper, we propose AFilter, an adaptable, and thus scalable, path expression filtering approach. AFilter has a base memory requirement linear in filter expression and data size. Furthermore, when additional memory is available, AFilter can exploit prefix commonalities in the set of filter expressions using a loosely-coupled prefix caching mechanism as opposed to tightly-coupled active state representation of alternative approaches. Unlike existing systems, AFilter can also exploit suffix-commonalities across filter expressions, while simultaneously leveraging the prefix-commonalities through the cache. Finally, AFilter uses a triggering mechanism to prevent excessive consumption of resources by delaying processing until a trigger condition is observed. Experiment results show that AFilter provides significantly better scalability and runtime performance when compared to state of the art filtering systems.
Frequent deletion of p16INK4a/MTS1 and p15INK4b/MTS2 in pediatric acute lymphoblastic leukemia (1995)

The tandemly linked p16INK4aMTS1 and p15INK4b/MTS2 genes on chromosome 9, band p21 encode proteins that function as specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. This locus undergoes frequent bi-allelic deletion in human cancer cell lines, suggesting that the encoded proteins may function as tumor suppressors. However, more recent analysis of primary tumor samples has shown a much lower frequency of abnormalities affecting this region, raising doubt over the importance of these proteins in human malignancies. Hemizygous deletions and rearrangements of chromosome 9, band p21, are among the most frequent cytogenetic abnormalities detected in pediatric acute lymphoblastic leukemia (ALL), occurring in approximately 10% of cases. To determine if the p16INK4a/p15INK4b locus might be the target of these chromosomal lesions, we analyzed both genes in primary clinical samples from 43 pediatric ALL patients using interphase fluorescence in situ hybridization, Southern blot analysis, and the polymerase chain reaction. Deletions of p16INK4a/p15INK4b were identified in 18 of 20 cases with cytogenetically observed abnormalities of 9p and 5 of 23 with apparently normal chromosomes 9p, with the majority containing bi-allelic deletions (16 homozygous/7 hemizygous). Although most homozygous deletions involved both genes, Southern blot analysis showed an interstitial deletion in a single case that was confined to p16INK4a, suggesting that p15INK4b was not the critical target gene in this case. Sequence analysis of both p16INK4a and p15INK4b in all seven cases with hemizygous deletions failed to show mutations within the coding regions of the retained alleles. In this select group of patients, deletion of p16INK4a/p15INK4b was associated with T-cell phenotype, nonhyperdiploid karyotype (< 50 chromosomes), and poor event-free survival. These findings indicate that deletion of the p16INK4a/p15INK4b locus is one of the most common genetic abnormalities so far detected in pediatric ALL, and that loss of one or more of these cell cycle kinase inhibitors is important in leukemogenesis.
A NEW SYNTHESIS OF AZIRIDINIUM SALTS. 2,2-PENTAMETHYLENE-1,1-TETRAMETHYLENEAZIRIDINIUM PERCHLORATE, A PROTOTYPE1 (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA NEW SYNTHESIS OF AZIRIDINIUM SALTS. 2,2-PENTAMETHYLENE-1,1-TETRAMETHYLENEAZIRIDINIUM PERCHLORATE, A PROTOTYPE1Nelson J. Leonard and Klaus JannCite this: J. Am. Chem. Soc. 1960, 82, 24, 6418–6419Publication Date (Print):December 1, 1960Publication History Published online1 May 2002Published inissue 1 December 1960https://pubs.acs.org/doi/10.1021/ja01509a061https://doi.org/10.1021/ja01509a061research-articleACS PublicationsRequest reuse permissionsArticle Views148Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
153. The oxidation of the silicon hydrides. Part II (1936)

H. J. Emeléus and K. Stewart, J. Chem. Soc., 1936, 677 DOI: 10.1039/JR9360000677
Analysis of cochannel interference in multi-cell OFDM networks (2002)

This paper provides an analytical framework for evaluating the performance of multi-cell orthogonal frequency division multiplexing (OFDM) networks in the presence of cochannel interference (CCI), frequency selective Rayleigh fading and additive white Gaussian noise (AWGN). Coherent and differential coherent modulation methods along with the imperfect power control mechanisms are considered in our analysis and the corresponding formulas of symbol error rate (SER) are derived. Moreover, forward error control coding schemes using bandwidth efficient inner trellis codes and outer Reed-Solomon codes are employed to improve system performance. The expressions of the asymptotic bit error rate (BER) of the inner trellis code and the SER of the concatenated code are analyzed. Finally, numerical results verify the effectiveness of the concatenated coding scheme in combating the channel fading and cochannel interference in OFDM networks.
Anti-Tumor Activity of IPI-504, a Novel Hsp90 Inhibitor in Multiple Myeloma. (2004)

Abstract IPI-504 is a novel inhibitor of Hsp90 based on the geldanamycin pharmacophore. When placed in rat, monkey, and human blood, IPI-504 rapidly converts to the known and well-studied compound 17-allylamino-17-demethoxy-geldanamycin (17-AAG). 17-AAG is the subject of multiple clinical trials for the treatment of hematologic and solid tumors. However, 17-AAG suffers from poor aqueous solubility necessitating the use of sub-optimal formulations to deliver this agent to patients. IPI-504 is over 1000-fold more soluble than 17-AAG in aqueous solution. In vitro, both 17-AAG and IPI-504 bind tightly to, and selectively inhibit Hsp90 derived from cancer cells. The cytotoxic effect of IPI-504, as well as its ability to stimulate the degradation of Hsp90 client proteins and increase the intracellular levels Hsp70, were monitored in two human multiple myeloma cells lines (RPMI-8226 and MM1.S). The effects of IPI-504 were compared to 17-AAG. We demonstrate that the actions of IPI-504 are bioequivalent to 17-AAG and that both compounds induce apoptosis in these cells and stimulate the degradation of HER2 and c-Raf. In addition, both agents stimulate Hsp70 protein levels. In all cases the EC50s are virtually the same for both molecules (~200–400 nM). Furthermore, IPI-504 inhibits the secretion of immunoglobulin light chain from the RPMI-8226 multiple myeloma cells (EC50 ~300 nM). Importantly, IPI-504 is active in tumor xenograft models of multiple myeloma. The data indicate that active metabolites of IPI-504 accumulate in these xenografts long after these metabolites are cleared from the plasma compartment, suggesting that they preferentially accumulate in tumor cells based on their increased affinity to Hsp90 derived from tumor cells. In conclusion, we have developed IPI-504 as a novel, potent inhibitor of Hsp90 with greatly increased solubility over 17-AAG, and that IPI-504 is an active anti-tumor agent in vitro and in vivo.