gebhart/patrepeval huggingface dataset:
Masitinib, a c‐kit/PDGF receptor tyrosine kinase inhibitor, improves disease control in severe corticosteroid‐dependent asthmatics (2009)

Masitinib, a c-kit and PDGF-receptor tyrosine kinase inhibitor, may represent an innovative avenue of treatment in corticosteroid-dependent asthma. These preliminary results warrant further long-term clinical studies in severe asthma
High Speed Packet Access Evolution - Concept and Technologies (2007)

In this paper we present the main concepts of high speed packet access evolution currently being standardized in 3GPP. In general HSPA evolution consists of introduction of MIMO, higher order modulation, and protocol optimizations and optimizations for voice over IP. We describe these improvements in detail and show that HSPA Evolution can reach performance comparable to those of long term evolution of UMTS terrestrial radio access network in a 5 MHz deployment.
Key Based Random Permutation (KBRP) (2006)

This study introduces a method for generating a particular permutation P of a given size N out of N! permutations from a given key. This method computes a unique permutation for a specific size since it takes the same key; therefore, the same permutation can be computed each time the same key and size are applied. The name of random permutation comes from the fact that the probability of getting this permutation is 1 out of N! possible permutations. Beside that, the permutation can not be guessed because of its generating method that is depending completely on a given key and size.
Children with disabilities in private inclusive schools in Mumbai: experiences and challenges (2010)

'Inclusive education’ policy has been introduced in India, however the concept is in its infancy This qualitative study analyses the case of children with disabilities studying in private inclusive schools of Mumbai. It discusses the development of self concept, elucidates the benefits and challenges of children with disabilities in inclusive education. We then suggest recommendations for improvements in implementing inclusive education in India.
A new approach to fabricate graphene nanosheets in organic medium: combination of reduction and dispersion (2010)

The direct dispersion of graphene nanosheets (GNS) in an organic medium, without assistance of dispersing agents, has been considered to be an insurmountable challenge. Herein we report a new approach to the fabrication of GNS by ethylenediamine (EDA) reduction in N,N-dimethylformamide (DMF). By this method, chemical reduction as well as dispersion of GNS can be carried out in one step. Characterization indicated that the bulk of oxygen-containing functional groups were removed from graphene oxide, and stable DMF colloidal suspensions containing homogeneously dispersed GNS were successfully obtained. Our findings make it possible to prepare graphene-based nanocomposites using a quick and low-cost solution processing technique.
Production of medium-chain carboxylic acids by Megasphaera sp. MH with supplemental electron acceptors (2016)

Megasphaera sp. MH metabolized fructose for the production of C2-C8 carbon-chain carboxylic acids using various electron acceptors and achieved a high-titer of 9.7 g L(-1) and fast productivity of 0.41 g L(-1) h(-1) for hexanoic acid. However, further metabolic activities of Megaspahera sp. MH for C5-C8 carboxylic acids production must be deciphered and improved for industrially relevant production levels.
Retroviral-mediated transfer of the human glucocerebrosidase gene into cultured Gaucher bone marrow. (1992)

Gaucher disease, a lysosomal glycolipid storage disorder, results from the genetic deficiency of an acidic glucosidase, glucocerebrosidase (GC). The beneficial effects of allogeneic bone marrow transplantation (BMT) for Gaucher disease suggest that GC gene transduction and the transplantation of autologous hematopoietic stem cells (gene therapy) may similarly alleviate symptoms. We have constructed a retroviral vector, L-GC, produced by a clone of the amphotropic packaging cell line PA317, which transduces the normal human GC cDNA with high efficiency. Whole-marrow mononuclear cells and CD34-enriched cells from a 4-yr-old female with type 3 Gaucher disease were transduced by the L-GC vector and studied in long-term bone marrow culture (LTBMC). Prestimulation of marrow with IL-3 and IL-6, followed by co-cultivation with vector-producing fibroblasts, produced gene transfer into 40-45% of the hematopoietic progenitor cells. The levels of GC expression in progeny cells (primarily mature myelomonocytic) produced by the LTBMC were quantitatively analyzed by Northern blot, Western blot, and glucocerebrosidase enzyme assay. Normal levels of GC RNA, immunoreactive protein, and enzymatic activity were detected throughout the duration of culture. These studies demonstrate that retroviral vectors can efficiently transfer the GC gene into long-lived hematopoietic progenitor cells from the bone marrow of patients with Gaucher disease and express physiologically relevant levels of GC enzyme activity.
Relationship of Job Performance to Job Perceptions of Salespersons (1981)

AbstractComparative perceptions of salespersons and sales managers with respect to dimensions of the formers' job were examined. It was shown that the two groups have similar perceptions regarding several dimensions, but that these perceptions relate only somewhat to subordinates' job performance and not at all to supervisors' performance.
Manipulations of cellular iron metabolism for modulating normal and malignant cell proliferation: achievements and prospects (1990)

in several biologic reactions and I is essential to virtually all forms of living organisms.
Secondary structure of human granulocyte colony‐stimulating factor derived from NMR spectroscopy (1992)

Recombinant 15N-, 13C-labeled human granulocyte colony-stimulating factor (rh-metG-CSF) has been studied by 2D and 3D NMR using uniformly labeled protein as well as residue-specific 15N-labeled samples. Assignment of the 1H, 15N backbone, and 60% 1H sidechain resonances has enabled the determination of the secondary structure of the protein. The secondary structure is dominated by alpha-helical regions with four stretches of helices between residues 11-41, 71-95, 102-124 and 144-170.
DNA Sequence Homology Between the Terminal Inverted Repeats of Shope Fibroma Virus and an Endogenous Cellular Plasmid Species (1986)

DNA hybridization experiments indicate that the genome of a tumorigenic poxvirus. Shope fibroma virus (SFV), possesses sequence homology with DNA isolated from uninfected rabbit cells. Southern blotting experiments, either with high-complexity rabbit DNA as probe and SFV restriction fragments as targets or with high-specific activity, 32P-labeled, cloned SFV sequences as probes and rabbit DNA as target, indicate that the homologous sequences map at two locations within the viral genome, one in each copy of the terminal inverted repeat sequences. Unexpectedly, Southern blots revealed that the homologous host sequences reside in a rabbit extrachromosomal DNA element. This autonomous low-molecular-weight DNA species could be specifically amplified by cycloheximide treatment and was shown by isopycnic centrifugation in cesium chloride-ethidium bromide to consist predominantly of covalently closed circular DNA molecules. DNA sequencing of pSIC-9, a cloned 1.9-kilobase fragment of the rabbit plasmid species, indicated extensive homology at the nucleotide level over a 1.5-kilobase stretch of the viral terminal inverted repeat. Analysis of open reading frames in both the plasmid and SFV DNA revealed that (i) the N-terminal 157-amino acid sequence of a potential 514-amino acid SFV polypeptide is identical to the N-terminal 157 amino acids of one pSIC-9 open reading frame, and (ii) a second long pSIC-9 open reading frame of 361 amino acids, although significantly diverged from the comparable nucleotide sequence in the virus, possessed considerable homology to a family of cellular protease inhibitors, including alpha 1-antichymotrypsin, alpha 1-antitrypsin, and antithrombin III. The potential role of such cellular plasmid-like DNA species as a mediator in the exchange of genetic information between the host cell and a cytoplasmically replicating poxvirus is discussed.
Identification of a Broadly Active Phage Lytic Enzyme with Lethal Activity against Antibiotic-Resistant Enterococcus faecalis and Enterococcus faecium (2004)

Enterococcus faecalis and Enterococcus faecium infections are increasingly difficult to treat due to high levels of resistance to antibiotics. PlyV12, a bacteriophage lytic enzyme, was isolated and shown to effectively kill both E. faecalis and E. faecium (including vancomycin-resistant strains), as well as other human pathogens. We propose its development and use as an alternative therapeutic tool.
Cell Cycle Checkpoint Proteins and Cellular Response to Treatment by Anticancer Agents (2004)

The response of cancer cells to treatment with anticancer agents is mediated in part by proteins controlling both the cell cycle progression and the genomic integrity, including p53, p73 and checkpoint proteins chk1 and chk2. We here summarized the cellular functions of these proteins, their alterations in human tumors and the impact of their mutations/alterations on cellular response to treatment, Particular attention has been paid for those studies performed in isogenic cell systems, to minimize as much as possible interference by other alterations invariably present when different cell types are considered. Focus has also be given to the approaches taken to exploit the differential expression of these proteins between normal and tumor cells to improve the selectivity of treatment with anticancer agents.
A Channel Aware Scheduling Algorithm for HSDPA System (2006)

High speed downlink packet access (HSDPA) is an evolutionary step to boost the WCDMA performance for downlink packet traffic as it increases spectral efficiency by supporting high user data rates. Because of the time varying channel fluctuations of wireless links, efficient use of the large available bandwidth cannot be achieved without adopting channel aware packet scheduling strategies. The HSDPA scheduling algorithm is based on splitting the scheduling decision into user selection and resource allocation. In this paper, we propose two user selection algorithms that can be adjusted (or pre-configured) in terms of cell throughput and/or user throughput fairness. Finally, we discuss a channel aware resource allocation algorithm that aims to maximize the number of served users among the selected ones, without any loss of the overall cell throughput with respect to the traditional greedy resource assignment.
Decidualization of human endometrial stromal cells in vitro: effects of progestin and relaxin on the ultrastructure and production of decidual secretory proteins* (1994)

Decidual cells arise by proliferation and differentiation of endometrial stromal cells of the uterus after appropriate stimulation by ovarian hormones. Previously we have shown that progestin and relaxin stimulate the secretion of several decidual-cell-specific secretory proteins in a long-term primary cell culture system. We now report the effects of progestin and relaxin on the morphology of stromal cells in association with the production rate of two major decidual secretory proteins, prolactin and insulin-like growth factor binding protein-1 (IGFBP-1). Stromal cells were cultured in RPMI 1640 and 2% fetal bovine serum for 22 days under control conditions (no hormone), with relaxin or medroxyprogesterone acetate (MPA), or MPA plus relaxin. Cells treated with MPA alone or MPA plus porcine relaxin grew to a high density with many areas of heaping while control cells and cells grown in medium containing relaxin alone formed discontinuous layers. The cytoplasm was distinguished by aggregates of rough endoplasmic reticulum and secretory granules. Surfaces of cells treated with MPA plus relaxin had clusters of short blunt processes containing secretory granules. The processes were rarely seen in cells exposed to MPA alone and completely absent in control cells or cells exposed to relaxin alone. Intercellular space was greatly widened in cells treated with MPA alone or MPA plus relaxin. There were many extended gap junctions in MPA- and relaxin-treated cells in contrast to controls.(ABSTRACT TRUNCATED AT 250 WORDS)
特輯(특집): 古代韓日關係(고대한일관계) 硏究(연구)의 새로운 흐름 : 倭國(왜국)의 第(제)3次(차) 遣唐使(견당사)와 百濟(백제),新羅(신라) (2013)

왜국의 견당사 역사에 있어서 654년 2월에 출발, 655년 8월에 귀국하는 제3차 견당사는 전후의 견당사와 비교해 두드러진 특징이 있다. 첫째, 사절의 성격이 전후의 견당사에 공통적인 정치문화사절이 아닌 순수한 정치외교사절이라는 점, 둘째, 653년 5월에 앞서 출발한 제2차 견당사의 귀국(654년 7월) 전에 긴급히 파견되었다는 점, 셋째, 파견의 직접적인 이유가 국내적 요인에 의해서라기보다는 국제적·외적요인에 의해 비롯되었다는 점, 넷째, 그 결과가 왜국의 대외정책, 특히 대신라정책상에 있어서 커다란 전환을 초래했다는 점 등이다. 본고는 이처럼 특이한 경우에 해당하는 제3차 견당사 문제에 주목해 파견의 배경 및 목적, 그리고 그 결과를 해명하기 위해 백제·신라의 움직임 및 당의 대응 등 국제적 요인과 왜국내의 권력주체의 변동이라는 국내적 요인 등을 종합적으로 고려하면서 검토해 보았다. 무엇보다도 제3차견당사의 제 문제를 특히 653년의 백제의 움직임에 주의하면서 왜국 내의 대(對)신라정책의 변화과정(=신라 「질」체제의 파탄과정)과 관련해 살펴보았다. 그 결과를 요약하면 다음과 같다. 첫째, 제3차 견당사 파견의 직접적이고 결정적인 외적 요인은 653년 6~8월에 걸친 백제의 대왜외교 강화이며, 이러한 백제의 대왜외교를 촉진시킨 중요한 계기는 (1)650년 신라 법민의 입당과 대백제원군요청, (2)이를 수용한 당의 백제에 대한 강경한 태도 표명, 즉 종래의 백제의 신라침공에 대한 최후통첩이라 할 수 있는 651년에 있어서의 당 황제의 璽書 전달이라 할 수 있다. 둘째, 동아시아의 급변하는 국제정세 속에서 654년 2월에 갑자기 파견된 제3차 견당사는 신라에서 상당 기간 체재하면서 원만하게 신라와의 외교절충을 걸친 후 입당하게 되는데 당에서 기다리고 있는 결과는 高向을 비롯한 견당사 일행이 전혀 예상하지 못한 것이었다. 즉, 그것은 당황제가 왜국왕에게 내린 청천벽력과도 같은 신라 구원, 대백제 출병이라는 출병명령이었다. 655년 8월에 귀국한 견당사 일행이 왜왕권에 전한 당의 명령은 왜국의 신라정책의 전환(신라 「질」 체제의 파탄)에 결정적으로 작용하게 된다. 이 왜국에 전해진 백제를 공격하라는 전혀 예기치 못한 당의 출병명령은 왜국으로 하여금 지금까지 개신정권이 추구해 온 외교정책 노선을 근본적으로 재검토하지 않으면 안 되도록 만들었기 때문이다. 왜냐하면 개신정권 성립에 의해 수립된 새로운 대신라정책의 핵심은 직접적인 대신라출병(=대백제 원군파견)을 자제한다는 전제 하에서 맺은 백제의 「질」 체제에 대신하는 신라의 .질. 도입책에 다름 아니었기 때문이다. 결국 왜국은 백제·고구려 라인과 신라·당 라인이라는 대립구도 속에서 양자택일을 하지 않으면 안 되는 상황에 놓이게 된 것이다. 왜국이 전자에 가담하지 않을 수 없었음은 물론이다. 셋째, 왜국 내에 있어서의 정치권력의 교체도 또한 앞서 언급한 국제적 요인과 함께 상호 작용을 하여 신라 「질」 체제의 파탄에 결정적으로 영향을 미쳤다고 할 수 있다. 즉 신라의 「질」 체제를 성립.유지시켜 왔던 당사자인 효덕(孝德)천황의 죽음, 그리고 그에 대신하는 새로운 권력주체의 등장인 중대형(中大兄)황자 및 제명(齊明)천황에 의한 권력 장악이다.
The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development (2008)

The vertebrate embryonic vasculature develops from angioblasts, which are specified from mesodermal precursors and develop in close association with blood cells. The signals that regulate embryonic vasculogenesis and angiogenesis are incompletely understood. Here, we show that R-spondin 3 (Rspo3), a member of a novel family of secreted proteins in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. In Xenopus embryos, morpholino antisense knockdown of Rspo3 induces vascular defects because Rspo3 is essential for regulating the balance between angioblast and blood cell specification. In mice, targeted disruption of Rspo3 leads to embryonic lethality caused by vascular defects. Specifically in the placenta, remodeling of the vascular plexus is impaired. In human endothelial cells, R-spondin signaling promotes proliferation and sprouting angiogenesis in vitro, indicating that Rspo3 can regulate endothelial cells directly. We show that vascular endothelial growth factor is an immediate early response gene and a mediator of R-spondin signaling. The results identify Rspo3 as a novel, evolutionarily conserved angiogenic factor in embryogenesis.
Network data mining: methods and techniques for discovering deep linkage between attributes (2006)

Abstract. Network Data Mining identifies emergent networks between myriads of individual data items and utilises special algorithms that aid visualisation of ‘emergent ’ patterns and trends in the linkage. It complements conventional data mining methods, which assume the independence between the attributes and the independence between the values of these attributes. These techniques typically flag, alert or alarm instances or events that could represent anomalous behaviour or irregularities because of a match with pre-defined patterns or rules. They serve as ‘exception detection ’ methods where the rules or definitions of what might constitute an exception are able to be known and specified ahead of time. Many problems are suited to this approach. Many problems however, especially those of a more complex nature, are not well suited. The rules or definitions simply cannot be specified. For example, in the analysis of transaction data there are no known suspicious transactions. This chapter presents a human-centred network data mining methodology that addresses the issues of depicting implicit relationships between data attributes and/or specific values of these attributes. A case study from the area of security illustrates the application of the methodology and corresponding data mining techniques. The chapter argues that for many problems, a ‘discovery’ phase in the investigative process based on visualisation and human cognition is a logical precedent to, and complement of, more automated ‘exception detection ’ phases.
Peptide-Based Biomaterials for Protease-Enhanced Drug Delivery (2006)

Controlled delivery of drugs in response to environments has the potential of targeting therapies and personalized treatments. Here, we described self-assembled peptide sequences that release therapeutic payloads upon specific interaction with disease-associated proteases. The core peptide sequence consists of a protease cleavable region flanked by two self-assembly motifs. In aqueous solution, the peptides self-assemble as a gel scaffold. With treatment of the model preparations with the appropriate protease, the matrix can be degraded in a controlled fashion, where the degradation rate is fine-tuned by varying the peptide compositions. Protease-mediated drug release was demonstrated by enzymatic treatment of a model therapeutic peptide incorporated into the optimized matrix. Our results suggest that this type of material may have far-reaching applications for functionally targeted drug delivery.
Yeast RNA Polymerase II Genes: Isolation with Antibody Probes (1983)

Genes encoding yeast RNA polymerase II subunits were cloned. Efficient isolation of these genes was accomplished by probing a phage lambda gt11 recombinant DNA expression library with polyvalent antibodies directed against purified yeast RNA polymerase II. The identity of genes that specify the largest RNA polymerase II subunits, the 220,000- and 150,000-dalton polypeptides, was confirmed by competitive radioimmune assay. Both of these genes exist in single copy in the yeast Saccharomyces cerevisiae.
Confocal fluorescence coincidence analysis: An approach to ultra high-throughput screening (1999)

Fluorescence-based assay technologies play an increasing role in high-throughput screening. They can be classified into different categories: fluorescence polarization, time-resolved fluorescence, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy. In this work we present an alternative analytical technique for high-throughput screening, which we call confocal fluorescence coincidence analysis. Confocal fluorescence coincidence analysis extracts fluorescence fluctuations that occur coincidently in two different spectral ranges from a tiny observation volume of below 1 fl. This procedure makes it possible to monitor whether an association between molecular fragments that are labeled with different fluorophores is established or broken. Therefore, it provides access to the characterization of a variety of cleavage and ligation reactions in biochemistry. Confocal fluorescence coincidence analysis is a very sensitive and ultrafast technique with readout times of 100 ms and below. This feature is demonstrated by means of a homogeneous assay for restriction endonuclease EcoRI. The presented achievements break ground for throughput rates as high as 10(6) samples per day with using only small amounts of sample substance and therefore constitute a solid base for screening applications in drug discovery and evolutionary biotechnology.
Cloning, Characterization, and Properties of Seven Triplet Repeat DNA Sequences (1996)

Several neuromuscular and neurodegenerative diseases are caused by genetically unstable triplet repeat sequences (CTG.CAG, CGG.CCG, or AAG.CTT) in or near the responsible genes. We implemented novel cloning strategies with chemically synthesized oligonucleotides to clone seven of the triplet repeat sequences (GTA.TAC, GAT.ATC, GTT.AAC, CAC.GTG, AGG.CCT, TCG.CGA, and AAG.CTT), and the adjoining paper (Ohshima, K., Kang, S., Larson, J. E., and Wells, R. D.(1996) J. Biol. Chem. 271, 16784-16791) describes studies on TTA.TAA. This approach in conjunction with in vivo expansion studies in Escherichia coli enabled the preparation of at least 81 plasmids containing the repeat sequences with lengths of approximately 16 up to 158 triplets in both orientations with varying extents of polymorphisms. The inserts were characterized by DNA sequencing as well as DNA polymerase pausings, two-dimensional agarose gel electrophoresis, and chemical probe analyses to evaluate the capacity to adopt negative supercoil induced non-B DNA conformations. AAG.CTT and AGG.CCT form intramolecular triplexes, and the other five repeat sequences do not form any previously characterized non-B structures. However, long tracts of TCG.CGA showed strong inhibition of DNA synthesis at specific loci in the repeats as seen in the cases of CTG.CAG and CGG.CCG (Kang, S., Ohshima, K., Shimizu, M., Amirhaeri, S., and Wells, R. D.(1995) J. Biol. Chem. 270, 27014-27021). This work along with other studies (Wells, R. D.(1996) J. Biol. Chem. 271, 2875-2878) on CTG.CAG, CGG.CCG, and TTA.TAA makes available long inserts of all 10 triplet repeat sequences for a variety of physical, molecular biological, genetic, and medical investigations. A model to explain the reduction in mRNA abundance in Friedreich's ataxia based on intermolecular triplex formation is proposed.
Phenyl Methimazole Inhibits TNF-α-Induced VCAM-1 Expression in an IFN Regulatory Factor-1-Dependent Manner and Reduces Monocytic Cell Adhesion to Endothelial Cells (2004)

Proinflammatory cytokine (e.g., TNF-alpha)-induced expression of endothelial cell adhesion molecules (ECAMs) on the lumenal surface of the vascular endothelium and a consequent increase in leukocyte adhesion are key aspects of pathological inflammation. A promising therapeutic approach to diminish aberrant leukocyte adhesion is, therefore, to inhibit cytokine-induced ECAM expression at the transcription level. Several studies suggest that methimazole, a compound used clinically to treat autoimmune diseases, such as Graves' disease, may also diminish pathological inflammation by suppressing ECAM expression. In this study we probed the hypothesis that a derivative of methimazole, phenyl methimazole (compound 10), can reduce cytokine-induced ECAM expression and consequent leukocyte adhesion. We found that compound 10 1) dramatically inhibits TNF-alpha-induced VCAM-1 mRNA and protein expression in human aortic endothelial cells (HAEC), has a relatively modest inhibitory effect on TNF-alpha induced E-selectin expression and has no effect on ICAM-1 expression; 2) significantly reduces TNF-alpha-induced monocytic (U937) cell adhesion to HAEC under in vitro flow conditions similar to that present in vivo; 3) inhibits TNF-alpha-induced IFN regulatory factor-1 binding to VCAM-1 promoter; and 4) reduces TNF-alpha-induced IRF-1 expression in HAEC. Combined, the results indicate that phenyl methimazole can reduce TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and that this contributes significantly to reduced monocytic cell adhesion to TNF-alpha-activated HAEC.