gebhart/patrepeval huggingface dataset:
Microbiota-Driven Immune Cellular Maturation Is Essential for Antibody-Mediated Adaptive Immunity to Staphylococcus aureus Infection in the Eye (2014)

As an immune-privileged site, the eye, and particularly the outer corneal surface, lacks resident mature immune effector cells. Physical barriers and innate mediators are the best-described effectors of immunity in the cornea. When the barriers are breached, infection can result in rapid tissue destruction, leading to loss of visual acuity and frank blindness. To determine the cellular and molecular components needed for effective adaptive immunity on the corneal surface, we investigated which immune system effectors were required for protection against Staphylococcus aureus corneal infections in mice, which are a serious cause of human eye infections. Both systemically injected and topically applied antibodies to the conserved cell surface polysaccharide poly-N-acetylglucosamine (PNAG) were effective at mediating reductions in corneal pathology and bacterial levels. Additional host factors impacting protection included intercellular adhesion molecule 1 (ICAM-1)-dependent polymorphonuclear leukocyte (PMN) recruitment, functional CD4(+) T cells, signaling via the interleukin-17 (IL-17) receptor, and IL-22 production. In germfree mice, there was no protective efficacy of antibody to PNAG due to the lack of LY6G(+) inflammatory cell coeffector recruitment to the cornea. Protection was manifest after 3 weeks of exposure to conventional mice and acquisition of a resident microbiota. We conclude that in the anterior eye, ICAM-1-mediated PMN recruitment to the infected cornea along with endogenous microbiota-matured CD4(+) T cells producing both IL-17 and IL-22 is required for antibody to PNAG to protect against S. aureus infection.
Status of Current Clinical Trials in Diabetic Polyneuropathy (2001)

Peripheral polyneuropathy is the most frequent complication of diabetic mellitus. In spite of many clinical trials of different specific interventions for diabetic polyneuropathy, intensive glycemic control remains the only effective specific therapy currently available for this troublesome complication. This systematic overview reports the status of current clinical trials in diabetic polyneuropathy with an emphasis on those interventions directed towards specific pathophysiological derangements. A discussion of clinical trials of agents directed towards relieving painful symptoms of diabetic polyneuropathy concludes this overview.
SST: Single-Stream Temporal Action Proposals (2017)

Our paper presents a new approach for temporal detection of human actions in long, untrimmed video sequences. We introduce Single-Stream Temporal Action Proposals (SST), a new effective and efficient deep architecture for the generation of temporal action proposals. Our network can run continuously in a single stream over very long input video sequences, without the need to divide input into short overlapping clips or temporal windows for batch processing. We demonstrate empirically that our model outperforms the state-of-the-art on the task of temporal action proposal generation, while achieving some of the fastest processing speeds in the literature. Finally, we demonstrate that using SST proposals in conjunction with existing action classifiers results in improved state-of-the-art temporal action detection performance.
Wirebonding: reinventing the process for MCMs (2002)

Summary form only given. Wirebonding is the mainstay interconnect for current IC products. Due to the standardization of pad metallurgy, wire types, package structures and bond optimization methods, wirebonding of individually packaged parts has achieved significant levels of quality and reliability. The situation in the multichip module (MCM) world is quite different. The introduction of the interconnection substrate with its typically different metallurgy, variable trace and pad geometries, multiple fabrication and attach process, and now in many cases a flexible or compliant (soft) structure has caused significant increases in wirebond failure rates and bondability concerns. We have conducted a systematic study of wirebonding processes (both thermosonic and ultrasonic) for multichip applications. The study included a variety of rigid, semi-rigid and compliant type substrate structures, both Si and GaAs chips, and even some exotic detector chips (CZT) and array structures. In this paper, we describe further results in bonding to soft or compliant substrate structures. Parameters investigated include bonding pad structures and morphology, the effect of ultrasonic delay on allowing compliant materials to recover after application of bonding force, and the influence of changing (increasing) the ultrasonic frequency from the standard 601 Hz. Again, the wirebond pull test and the ball bond shear test are used as measures for improvement. Strength maintenance as a function of aging at elevated temperature is used as an indicator of reliability.
Analysis and Design of Forced Commutated Cycloconverter Structures with Improved Transfer Characteristics (1986)

Forced Commutated Cycloconverters (FCC's) are converter structures capable of providing simultaneous voltage and frequency transformation without the need of intermediate stage reactive components. Consequently, they are well-suited for high-power density variable-frequency applications.
Purification and characterization of an activator protein for methanol dehydrogenase from thermotolerant Bacillus spp (1991)

All thermotolerant methanol-utilizing Bacillus spp. investigated by us possess a NAD-dependent methanol dehydrogenase (MDH) activity which is stimulated by a protein present in the soluble fraction of Bacillus sp. C1 cells. This activator protein was purified to homogeneity from Bacillus sp. C1 cells grown at a low dilution rate in a methanol-limited chemostat culture. The native activator protein (Mr = 50,000) is a dimer of Mr = 27,000 subunits. The N-terminal amino acid sequence revealed no significant similarity with any published sequences. Stimulation of MDH activity by the activator protein required the presence of Mg2+ ions. Plots of specific MDH activity versus activator protein concentration revealed Michaelis-Menten type kinetics. In the presence of activator protein, MDH displayed biphasic kinetics (v versus substrate concentration) toward C1-C4 primary alcohols and NAD. The data suggest that in the presence of activator protein plus Mg2+ ions, MDH possesses a high affinity active site for alcohols and NAD, in addition to an activator- and Mg2(+)-independent low affinity active site. The activation mechanism remains to be elucidated.
The use of adenovirus vectors for intracerebral grafting of transfected nervous cells (1994)

Grafting genetically-modified cells into the brain is a promising approach to address fundamental and clinical issues in neurobiology. Despite recent substantial progress, most of the methods used for introducing DNA sequences into donor cells result in weak efficacy or transient gene expression after transplantation. We tested whether the use of adenovirus as the vector for foreign genes avoided these problems. A replication-defective adenovirus vector carrying a reporter gene encoding for beta-galactosidase was used to transfect primary astrocytes. After grafting into various brain structures, transfected cells exhibited robust survival and expressed the transgene for at least five months. These results demonstrate the advantage of adenovirus-mediated gene transfer for prolonged transgene expression in grafted primary cells.
The CD94 and NKG2‐A C‐type lectins covalently assemble to form a natural killer cell inhibitory receptor for HLA class I molecules (1997)

CD94, a type II membrane protein containing a C-type lectin domain, has been shown to be involved in natural killer (NK) cell-mediated recognition of different HLA allotypes. The inhibitory form of the CD94 receptor has recently been identified by the specific monoclonal antibody (mAb) Z199. Herein, we demonstrate that the inhibitory receptor is in fact a complex formed by the covalent association of CD94 with the NKG2-A molecule (Mr approximately 43 kDa), another member of the C-type lectin superfamily, and that Z199 mAb specifically recognize NKG2-A molecules. Although the NKG2-A-encoding cDNA has been known for several years, the corresponding protein and its possible function remained undefined. Moreover, we show that the NKG2-B protein, an alternatively spliced product of the NKG2-A gene, can also assemble with CD94. Remarkably, both NKG2-A and NKG2-B proteins contain cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIM). This may provide the molecular basis of the inhibitory function mediated by the CD94/NKG2-A receptor complexes.
The metabolism of tryptophan. 2. The metabolism of tryptophan in patients suffering from cancer of the bladder (1956)

Research Article| November 01 1956 The metabolism of tryptophan. 2. The metabolism of tryptophan in patients suffering from cancer of the bladder* E. Boyland; E. Boyland 1Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, London, S.W. 3 Search for other works by this author on: This Site PubMed Google Scholar D. C. Williams D. C. Williams 1Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, London, S.W. 3 Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1956) 64 (3): 578–582. https://doi.org/10.1042/bj0640578 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation E. Boyland, D. C. Williams; The metabolism of tryptophan. 2. The metabolism of tryptophan in patients suffering from cancer of the bladder. Biochem J 1 November 1956; 64 (3): 578–582. doi: https://doi.org/10.1042/bj0640578 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1956 CAMBRIDGE UNIVERSITY PRESS1956 Article PDF first page preview Close Modal You do not currently have access to this content.
Observation of Precessional Magnetization Dynamics in L10-FePt Thin Films with Different L10 Order Parameter Values (2013)

Fast magnetization precession was observed in L1 0 -FePt thin films with different L1 0 order parameter values by all optical pump–probe technique. Precession frequency was varied widely for the films with different order parameter, which is due to large difference in perpendicular magnetic anisotropy. Gilbert damping constant (α) was estimated from relaxation time as apparent damping. Clear difference in α was not observed with different perpendicular magnetic anisotropy.
Armored RNA Technology for Production of Ribonuclease-Resistant Viral RNA Controls and Standards (1998)

The widespread use of sensitive assays for the detection of viral and cellular RNA sequences has created a need for stable, well-characterized controls and standards. We describe the development of a versatile, novel system for creating RNase-resistant RNA. "Armored RNA" is a complex of MS2 bacteriophage coat protein and RNA produced in Escherichia coli by the induction of an expression plasmid that encodes the coat protein and an RNA standard sequence. The RNA sequences are completely protected from RNase digestion within the bacteriophage-like complexes. As a prototype, a 172-base consensus sequence from a portion of the human immunodeficiency virus type 1 (HIV-1) gag gene was synthesized and cloned into the packaging vector used to produce the bacteriophage-like particles. After production and purification, the resulting HIV-1 Armored RNA particles were shown to be resistant to degradation in human plasma and produced reproducible results in the Amplicor HIV-1 Monitor assay for 180 days when stored at -20 degreesC or for 60 days at 4 degreesC. Additionally, Armored RNA preparations are homogeneous and noninfectious.
The gene therapy journey for hemophilia: are we there yet? (2012)

Since the isolation and characterization of the genes for FVIII and FIX some 30 years ago, a longstanding goal of the field has been development of successful gene therapy for the hemophilias. In a landmark study published in 2011, Nathwani et al demonstrated successful conversion of severe hemophilia B to mild or moderate disease in 6 adult males who underwent intravenous infusion of an adeno-associated viral (AAV) vector expressing factor IX. These 6 subjects have now exhibited expression of FIX at levels ranging from 1% to 6% of normal for periods of > 2 years. This review discusses obstacles that were overcome to reach this goal and the next steps in clinical investigation. Safety issues that will need to be addressed before more widespread use of this approach are discussed. Efforts to extend AAV-mediated gene therapy to hemophilia A, and alternate approaches that may be useful for persons with severe liver disease, who may not be candidates for gene transfer to liver, are also discussed.
R-spondins: Novel matricellular regulators of the skeleton (2014)

R-spondins are a family of four matricellular proteins produced by a variety of cell-types. Structurally, R-spondins contain a TSR1 domain that retains the tryptophan structure and a modified cysteine-rich CSVCTG region. In addition, the R-spondins contain two furin repeats implicated in canonical Wnt signaling. R-spondins positively regulate canonical Wnt signaling by reducing Wnt receptor turnover and thereby increasing beta-catenin stabilization. R-spondins are prominently expressed in the developing skeleton and contribute to limb formation, particularly of the distal digit. Additionally, results suggest that R-spondins may contribute to the maintenance of adult bone mass by regulating osteoblastogenesis and bone formation.
Alterations in serum immunoglobulin G subclasses in patients with ulcerative colitis and Crohn's disease. (1989)

We have examined the concentration of immunoglobulin G (IgG) subclass antibodies in the sera of 27 patients with ulcerative colitis and 21 patients with Crohn's disease as well as in 11 normal controls and 11 patients with systemic lupus erythematosus. In comparison with a control mean serum IgG1 concentration of 5173 micrograms/ml, patients with ulcerative colitis exhibited a significantly increased mean serum concentration of 7924 micrograms/ml (p less than 0.05), whereas patients with Crohn's disease had a near normal mean serum IgG1 level of 5898 micrograms/ml. In contrast, control sera had a mean IgG2 level of 2477 micrograms/ml and ulcerative colitis sera had a similar IgG2 level of 2269 micrograms/ml, whereas Crohn's disease sera had a significantly increased mean IgG2 level of 5111 micrograms/ml (p less than 0.05). Patients with systemic lupus erythematosus, like those with ulcerative colitis, had a markedly elevated serum IgG1 level of 15,594 micrograms/ml (p less than 0.001) without a significantly increased IgG2 serum level (3271 micrograms/ml). Neither ulcerative colitis nor Crohn's disease sera exhibited altered levels of IgG3 or IgG4. These data show that alterations in IgG subclass concentrations occur in the sera of patients with active, untreated inflammatory bowel disease, similar to the previously noted changes in the IgG subclasses secreted by lymphocytes from involved inflammatory bowel disease intestinal specimens.
Uniaxial mechanical tension promoted osteogenic differentiation of rat tendon‐derived stem cells (rTDSCs) via the Wnt5a‐RhoA pathway (2012)

Chronic tendinopathy is a tendon disorder that is common in athletes and individuals whose tendons are subjected to repetitive strain injuries. The presence of ossification worsened the clinical manifestation of the disorder. The change of tendon loading due to mechanical overload, compression, or disuse have been implicated as the possible etiologies, but the pathological mechanisms of tendinopathy remain unclear. In this study, we demonstrated that ossification in tendon tissue might be due to the osteogenesis of tendon-derived stem cells (TDSCs) induced by uniaxial mechanical tension (UMT) which mimics the mechanical loading in tendon. Rat TDSCs (rTDSCs) could be induced to differentiate into osteogenic lineage after treatment with 2% elongation UMT for 3 days as shown by the increased expression Runx2 mRNA and protein, Alpl mRNA, collagen type 1 alpha 1 (Col1a1) mRNA, ALP activity, and ALP cytochemical staining. RhoA, an osteogenesis regulator, was activated in rTDSCs upon UMT stimulation. Blockage of RhoA activity in rTDSCs by C3 toxin or ROCK activity, a downstream target of RhoA, by Y-27632 inhibited UMT-induced osteogenesis in rTDSCs. UMT up-regulated the mRNA expression of Wnt5a but not the other non-canonical Wnts. The inhibition of Wnt5a expression by siRNA abolished UMT-induced Runx2 mRNA expression and RhoA activation in rTDSCs and the inhibition of Runx2 expression could be rescued by addition of LPA, a RhoA activator. In conclusion, our results showed that UMT induced osteogenic differentiation of rTDSCs via the Wnt5a-RhoA pathway, which might contribute to ectopic ossification in tendon tissue due to mechanical loading.
Solidarity through National Pride: The Future of Catholic Politics in the 21st Century (2019)

This paper raises the Pragmatist concepts of solidarity and national pride, as espoused by American philosophers such as John Dewey and Richard Rorty, as potential means for Catholics in Western democratic nations to approach the current political issues facing them in the 21st century. Though Dewey and Rorty were neither politicians nor Catholics (being liberal thinkers trained in philosophy), their views on solidarity and patriotism in modern liberal democracies provide useful roadmaps for Catholics in Europe and the Anglosphere to navigate our present period of polarised and highly partisan politics, potentially reaching a ‘sensible centre’ akin to the American Catholic political tradition since the 1930s. This centrism relies on this Pragmatist solidarity through a shared hope of a pluralistic society constantly improved upon for the benefit of future generations. Catholics can only achieve this solidarity and hope if they feel, in the spirit of Rorty, a deep sense of national pride for their country. This type of national pride is not reactionary in nature, but advocates a politics of pluralism rather than identity, democratic nationalism rather than amorphous internationalism, and active engagement in the public square to implement an achievable political programme of action which is hopeful and borne out of a collective imagination for a better future for their countries.
Comparative Effects of Antacids, Cimetidine and Enteric Coating on the Therapeutic Response to Oral Enzymes in Severe Pancreatic Insufficiency (1977)

To provide a rational basis for pancreatic enzyme replacement therapy, we evaluated, in six patients with advanced pancreatic insufficiency, the effects of various treatment regimens on fecal fat and nitrogen balance and on duodenal recovery of ingested pancreatic enzymes after a solid test meal. The combination of cimetidine (an H2-receptor antagonist) and pancreatin, each given by mouth, produced significantly higher postprandial duodenal recoveries and concentrations of trypsin and lipase (P<0.05). Steatorrhea was reduced in all patients and abolished in four of the six. In the dosages used, neither entericcoated enzymes nor supplemental neutralizing antacids were more effective than pancreatin alone in decreasing steatorrhea or improving duodenal enzyme delivery. Cimetidine may be a useful adjunct to oral pancreatic extract therapy in some patients with severe pancreatic insufficiency who fail to respond to pancreatic enzyme replacement alone. (N Engl J Med 297:854–858, 1977)
Effect of Organic Effectors on Chromatin Solubility, DNA-Histone H1 Interactions, DNA and Histone HI Structures (1993)

We have extended our previous investigations on the effect of organic osmolytes (glycine, proline, taurine, mannitol, sorbitol and trimethylammonium oxide (TMAO)) on chromatin solubility, to the study of their influence on DNA stability and DNA-histone interactions. Our aim was to understand the molecular origin of the protection effects observed. To this end, we determined the amount of histone H1 required to precipitate DNA or H1-depleted chromatin, at various salt concentrations, in the presence of the above mentioned organic compounds. We found a shift of the H1/DNA ratio required to reach 50% precipitation, towards higher values. Taurine was the most efficient compound followed by mannitol and glycine, then sorbitol and proline. On the contrary, TMAO favoured the precipitation process. We attempted to interpret these results on the basis of Manning's counterion condensation theory. Changes in histone H1 structure folding and in DNA melting temperature Tm were also analyzed. Glycine, taurine, sorbitol and TMAO increased the degree of secondary structure folding of the protein while mannitol and sorbitol had no effect. Taurine, glycine and proline decreased the Tm of DNA, TMAO largely destabilized DNA, but mannitol and sorbitol had no effect. Measurements of NaCl activity in the presence of organic osmolytes did not reveal sufficiently large changes to account for their protection effect against chromatin precipitation. The osmotic coefficient j of the organic effectors solutions increased in the order: taurine < glycine < sorbitol < mannitol < proline << TMAO. For the two latter compounds, the j values increased above 1 at high concentration. We consider that the organic compounds investigated may be classified into three categories: (i) class I (zwitterionic compounds: glycine, proline, taurine) would produce sodium ions release from the DNA surface; (ii) class II (the very polar molecule TMAO) would increase sodium counterions condensation on DNA together with histone H1 folding; (iii) class III compounds (mannitol and sorbitol) would possibly produce a modification of NaCl activity but no definite explanation could be found for the complex behavior of these compounds.
A Rapid and Nondestructive Plant Nutrient Analysis using Portable X-Ray Fluorescence (2012)

Portable X-ray fluorescence (PXRF) can provide a rapid and nondestructive analysis for environmentally important elements in plant matrices. No previous publication has been able to demonstrate the application of PXRF element determination in plant material. To assess the applicability of PXRF for total element analysis, four plant species of agricultural importance {corn (maize) [Zea mays L.], cotton [Gossypium hirsutum L.], soybean [Glycine max (L.) Merr.], and wheat [Triticum aestivum L.]} were collected across northern New South Wales (NSW), Australia. The effect of scanning time and particle size on data quality was also evaluated. For three plant species (corn, cotton, and soybean) there were significant linear relationships between the acid digest values and the PXRF readings for Ca, Co, Cr, Fe, K, Mn, Ni, P, S, Si, and Zn. Only slight reductions in the regression coefficient (r2) for Fe and Zn were observed in cotton when scanning time was reduced by more than half. Similar regressions between corn and cotton at 120 s demonstrated the potential for a single algorithm for plant matrices. The use of novel techniques to optimize PXRF settings for quantitative determination of total plant elements provides an efficient alternative to traditional plant digestion.