gebhart/patrepeval huggingface dataset:
Stably tethered multifunctional structures of defined composition made by the dock and lock method for use in cancer targeting (2006)

We describe a platform technology, termed the dock and lock method, which uses a natural binding between the regulatory subunits of cAMP-dependent protein kinase and the anchoring domains of A kinase anchor proteins for general application in constructing bioactive conjugates of different protein and nonprotein molecules from modular subunits on demand. This approach could allow quantitative and site-specific coupling of many different biological substances for diverse medical applications. The dock and lock method is validated herein by producing bispecific, trivalent-binding complexes composed of three stably linked Fab fragments capable of selective delivery of radiotracers to human cancer xenografts, resulting in rapid, significantly improved cancer targeting and imaging, providing tumor/blood ratios from 66 +/- 5 at 1 h to 395 +/- 26 at 24 h.
Acute renal failure in hospitalized patients. (2002)

Distinguishing among the three categories of acute renal failure is important, as the evaluation and management are tailored to the particular cause. Most cases are due to acute tubular necrosis. To minimize the risk, we should give hospital patients adequate hydration, use potentially nephrotoxic drugs with caution, keep the use of radiographic contrast agents to a minimum, and give patients at risk a nonionic instead of ionic contrast agent when undergoing radiographic procedures.
Biomimetic Transformation of Hodgkinsine, a Pyrrolidinoindoline Alkaloid (1988)

a -The msfonnatim of hcdgkinsine 1 under mild acidic conditions provide5 a new compound whose smctun has becn shown to possess calycanthine and pynolidinomdoline subunits.This compound could be identified with calycosidine 2 previously isolated from Calycodendron milnei.General implications of this reaction are discussed.In a preliminary paper' we reported the isolation of hcdgkinsine 1 along with calycosidine 2, a new minor indole alkaloid hwn thc stem bark of Calycodendron milnei .The biological activify of several canpounds belonging to this family of indole alkaloids has been previously described and antibiotic activity 2, human platelet aggregation inhibition3, cytotoxicity 4, as well as activity on the cenu;~l nervous system has k e n demonstrated.We therefore set out to study chemical uansform:~t~nn\ of hodgkinsine 1, whose stlucture had been ascertained by X-ray analysis6, showing it to possess three Nmethylpynolidinoindoline units as depicted in formula I. hcdgkinsinePrevious studies had established that the acid catalyzed isomerisation of (-)-chimonanthine 3, meso- chimonanthine 4 and racemic chimonanthine 5 led to respectively (+)-calycanthine 6 7, meso-calycanthine 7 and racemic calycanthine l I 7 , ' , as !heonly obsemed rcaction products.A mechanism has been proposed for this reaction (Figure 1) which allows for the retention of configurat~on of carbon atoms 3a and 3a' in these
Assessing Platelet and Fibrinogen Contribution to Clot Strength Using Modified Thromboelastography in Pregnant Women (1999)

The monoclonal antibody fragment c7E3 Fab (ReoPro®), by binding to platelet surface fibrinogen receptors (glycoprotein, GPIIb/IIIa), inhibits platelet aggregation and its interaction with fibrinogen. In this study, we used thromboelastography with ReoPro® to evaluate the independent contribution of fibrinogen and platelets to clot strength. Thromboelastography was performed in 21 healthy, term parturients scheduled for elective cesarean delivery with 360 μL of celite-activated whole blood and with 5 μL of (2 mg/mL) ReoPro® added to 355 μL of celite-activated whole blood. The contribution of platelets to clot strength (MAplt) was derived by subtracting MAfib (maximal amplitude with ReoPro®) from MAwb (maximal amplitude with whole blood). Thus, MAwb − MAfib = MAplt. The value for MAwb (mean ± SD) was 73 ± 4 mm, for MAfib it was 33 ± 5 mm, and for MAplt it was 40 ± 3 mm. The contribution of fibrinogen and platelets to the MAplt was 45% and 55%, respectively. Modified thromboelastography using ReoPro® in healthy parturients can be used to determine the contribution of fibrinogen and platelets to blood clot strength. Implications Determining the independent contribution of platelets and fibrinogen to the maximal amplitude of thromboelastography using c7E3 Fab may further improve the use of thromboelastography in detecting and treating coagulation defects.
Triple-Shape Memory Polymers Based on Self-Complementary Hydrogen Bonding (2012)

Triple shape memory polymers (TSMPs) are a growing subset of a class of smart materials known as shape memory polymers, which are capable of changing shape and stiffness in response to a stimulus. A TSMP can change shapes twice and can fix two metastable shapes in addition to its permanent shape. In this work, a novel TSMP system comprised of both permanent covalent cross-links and supramolecular hydrogen bonding cross-links has been synthesized via a one-pot method. Triple shape properties arise from the combination of the glass transition of (meth)acrylate copolymers and the dissociation of self-complementary hydrogen bonding moieties, enabling broad and independent control of both glass transition temperature (T(g)) and cross-link density. Specifically, ureidopyrimidone methacrylate and a novel monomer, ureidopyrimidone acrylate, were copolymerized with various alkyl acrylates and bisphenol A ethoxylate diacrylate. Control of T(g) from 0 to 60 °C is demonstrated: concentration of hydrogen bonding moieties is varied from 0 to 40 wt %; concentration of the diacrylate is varied from 0 to 30 wt %. Toughness ranges from 0.06 to 0.14 MPa and is found to peak near 20 wt % of the supramolecular cross-linker. A widely tunable class of amorphous triple-shape memory polymers has been developed and characterized through dynamic and quasi-static thermomechanical testing to gain insights into the dynamics of supramolecular networks.
Biaxial Flow-Induced Alignment of Silicate Layers in Polypropylene/Clay Nanocomposite Foam (2001)

Via batch process in an autoclave, we conducted foam processing on polypropylene (PP)/clay nanocomposite (PPCN) by using supercritical CO2 as foaming agent under 10 MPa at 134.7 °C. Through transmission electron microscopy observation, the biaxial flow-induced alignment of clay particles along the cell boundary was identified. Such aligning behavior of clay particles helps cells to withstand the stretching force from breaking the so thin cell wall and to improve the modulus of the foam.
Compact 1-W Yb-doped double-cladding fiber amplifier using V-groove side-pumping (1998)

A compact packaged fiber amplifier was constructed using a Yb-doped double-cladding fiber, which was V-groove side-pumped by a laser diode operating at 975 nm. Under saturation, the amplifier generated 1 W at 1064 nm. The small-signal gain reached a maximum of 50 dB at 1075 nm and was greater than 40 dB in the range of 1042-1093 nm.
Evolution and Functional Impact of Rare Coding Variation from Deep Sequencing of Human Exomes (2012)

As a first step toward understanding how rare variants contribute to risk for complex diseases, we sequenced 15,585 human protein-coding genes to an average median depth of 111× in 2440 individuals of European (n = 1351) and African (n = 1088) ancestry. We identified over 500,000 single-nucleotide variants (SNVs), the majority of which were rare (86% with a minor allele frequency less than 0.5%), previously unknown (82%), and population-specific (82%). On average, 2.3% of the 13,595 SNVs each person carried were predicted to affect protein function of ~313 genes per genome, and ~95.7% of SNVs predicted to be functionally important were rare. This excess of rare functional variants is due to the combined effects of explosive, recent accelerated population growth and weak purifying selection. Furthermore, we show that large sample sizes will be required to associate rare variants with complex traits.
Swainsonine, an Inhibitor of Glycoprotein Processing, Enhances Cytotoxicity of Large Granular Lymphocytes (1990)

In the present study we investigated the effects of inhibitors of glycoprotein processing on cytotoxicity of human large granular lymphocytes (LGL). The incubation of LGL for 36 h with 0.5 microgram/ml swainsonine (SW), which is an inhibitor of mannosidase II, resulted in the augmentation of cytotoxicity of LGL against an NK-resistant colon carcinoma cell line (Colo-320DM) without increase of binding frequency of LGL to target cells or of cell proliferation. The enhanced cytotoxicity was associated with increased binding of concanavalin A to SW-treated LGL. The augmentation of cytotoxicity was also seen by 1-deoxymannojirimycin (1-DMN), an inhibitor of mannosidase I, but much higher amounts of this agent were needed to get the same level of augmentation as that with SW. Other inhibitors of glycoprotein processing such as castanospermine and 1-deoxynojirimycin (1-DN) did not show any augmentative effects on LGL cytotoxicity. The enhancement of cytotoxicity by SW was abolished by the addition of rabbit anti-human interleukin (IL-2) antibody to the culture. This result suggests that IL-2 is involved in the augmentation of cytotoxicity of LGL by SW. The presence of SW in the culture of LGL together with IL-2 also enhanced LAK generation compared to that with IL-2 alone. Thus, our results suggest that SW should be recognized as an efficient immunopotentiator and that modulation of carbohydrate moieties elicited by SW may shed further light on the mechanism of LGL activation.
The importance of a distal hydrogen bonding group in stabilizing the transition state in subtilisin BPN' (1991)

Stabilization of an oxyanion transition state is important to catalysis of peptide bond hydrolysis in all proteases. For subtilisin BPN', a bacterial serine protease, structural data suggest that two hydrogen bonds stabilize the tetrahedral-like oxyanion intermediate: one from the main chain NH of Ser221 and another from the side chain NH2 of Asn155. Molecular dynamic studies (Rao, S., N., Singh, U., C. Bush, P. A., and Kollman, P. A. (1987) Nature 328, 551-554) have indicated the gamma-hydroxyl of Thr220 may be a third hydrogen bond donor even though it is 4A away in the static x-ray structure. We have probed the role of Thr220 by replacing it with serine, cysteine, valine, or alanine by site-directed mutagenesis. These substitutions were intended to alter the size and hydrogen bonding ability of residue 220. Removal of the gamma-hydroxyl group reduced the transition state stabilization energy (delta delta GT) by 1.8-2.1 kcal/mol depending upon the substitution. By comparison, removal of the gamma-methyl group in the Thr220 to serine mutation only decreased delta GT by 0.5 kcal/mol. The gamma-hydroxyl of Thr220 is most important for catalysis, not substrate binding, because virtually all of the effects were on kcat and not KM. The role of the Thr220 hydroxyl is functionally independent from the amide NH2 of Asn155 because the free energy effects of double alanine mutants at these two positions are additive. These data indicate that a distal hydrogen bond donor, namely the hydroxyl of Thr220, plays a functionally important role in stabilizing the oxyanion transition state in subtilisin which is independent of Asn155.
Dactylocyclines, novel tetracycline derivatives produced by a Dactylosporangium sp. I. Taxonomy, production, isolation and biological activity. (1992)

A screen for antibiotics with activity against tetracycline-resistant microorganisms has led to the isolation of Dactylosporangium sp. (ATCC 53693), a producer of several novel tetracycline derivatives. The major fermentation products, dactylocyclines A and B, were purified and MIC values determined against tetracycline-resistant and tetracycline-sensitive Gram-positive bacteria. The dactylocyclines represent the first naturally occurring tetracycline C2 amides which lack cross resistance with tetracycline.
Evaluation of the Arginine Maturity Index (AMI) Method of Maturity Estimation for Virginia Type Peanuts1 (1976)

Abstract The arginine maturity index (AMI) method of predicting maturity in peanuts was evaluated on Virginia type peanuts grown in the North Carolina-Virginia area. Analyses of data from a two year study showed statistical differences (P &amp;gt; .05) of AMI values for cultivars, location and digging dates. Official grade factors suggested maturity levels consistent with AMI maturity predictions. Maximum yields per acre were observed near minimum AMI values. Optimum yields and dollars per acre were observed primarily at AMI values of 100. However, no sufficiently definitive value was determined to be the recommended point for digging peanuts. The AMI method was tested on commercial grades of shelled peanuts. Significant differences (P &amp;gt; .05) between AMI values of cultivars were also observed.
Gentamicin-Induced Correction of CFTR Function in Patients with Cystic Fibrosis andCFTRStop Mutations (2003)

In patients with cystic fibrosis who have premature stop codons, gentamicin can cause translational "read through," resulting in the expression of full-length CFTR protein at the apical cell membrane, and thus can correct the typical electrophysiological abnormalities caused by CFTR dysfunction.
Input-shaped control of three-dimensional maneuvers of flexible spacecraft (1993)

This paper deals with the control of three-dimensional rotational maneuvers of flexible spacecraft. A spacecraft model with a cylindrical huh and four symmetric appendages is considered. The appendages are tong and flexible, leading to low-frequency vibration under any control action. To provide a comprehensive treatment of input-shaped controllers (time-delay filters), both open-loop and closed-loop maneuvers are considered. For the open-loop maneuver, a five-switch, near-minimum-time bang-bang controller is designed based on the rigid-body model. The design procedure accounts for the presence of the time-delay filter for determining the switch times. In addition, a combination of a Lyapunov controller with the time-delay control technique is proposed to take advantage of the simple feedback control strategy and augment it with a technique that can eliminate the vibratory motion of the flexible appendages more efficiently. I. Introduction N this paper the problem of reorientation of a hub-apI pendage structure is considered. Considerable work has been done on Lyapunov control of single-axis maneuvers of hub-appendage structures. Hablani5 provides a technique for the estimation of the switch times for a bang-bang profile for zero residual energy, single-axis slew of a flexible spacecraft. Techniques are provided to estimate switch times for a system with either damped or undamped modes. A weighted time-fuel optimal controller for the rest-to-rest slew with zero residual energy is designed using the principles of dynamics by Skaar et a1.6 An elegant input-shaping scheme proposed by Smith 7 and later modified by Singer and Seering* has been used by Wie and Liu9 to modify the flexible-body time optimal control profile to produce a robust control scheme, which has been applied to the control of a two-mass spring model. Here we consider the three-dimensional case, leading to a model that has kinematic and dynamic nonlinearities. The system under consideration consists of a cylindrical hub, with four cantilevered cylindrical appendages of equal dimensions (Fig. 1). In modeling this system, only those modes that will be excited by a hub torque are considered. These modes lead to displacements of the appendages (which are modeled as EulerBernoulli beams), which resemble a three-dimensional starfish. The equations of motion of the flexible appendages are arrived at using the Lagrangian approach, and the rigidbody equations are arrived at using the Newton-Euler technique. The assumed modes technique is used to transform the partial differential equations (representing the equations of motion of the flexible appendages) to ordinary differential equations. Two modes are used to represent the displacement of each of the flexible appendages in the simulation of the system. The first controller proposed in this study is based on the amalgamation of two powerful control techniques. First, the rigid-body controller is designed based on the Lyapunov sta
Combination Therapy (2003)

Adult Treatment Panel III (ATP III) guidelines recommend specific treatment targets for low-density lipoprotein cholesterol (LDL-C) levels according to an individual.s short-term and long-term risk for coronary heart disease (CHD). Therapeutic lifestyle changes are recommended for all patients at any level of risk for CHD. Although most patients will achieve some LDL-C lowering with lifestyle modification, ATP III recognizes that a majority of patients with dyslipidemia will require drug therapy to reach their LDL goal. Surveys of physicians. practices suggest that only a small percentage of patients enrolled in an active treatment program actually achieve their LDL-C target. In addition, other surveys suggest that not all patients who are treatment candidates are receiving assessment. From a medication perspective, either up-titration of statin dose or the use of drug combinations should further enhance the likelihood of achieving target lipid levels. Combination therapies that target both the endogenous and exogenous pathways of cholesterol synthesis are particularly attractive. This paper reviews the pharmacotherapeutic effects of combination therapy, summarizes the strengths and weaknesses of current lipid-lowering drug combinations, and identifies the potential contribution of the novel cholesterol absorption inhibitor, ezetimibe, to the LDL-C treatment algorithm.
Lymphoid Monoclonal Antibodies Reactive with Lung Tumors (1996)

In the course of investigating 30 monoclonal antibodies (MAbs) for their potential reactivity with 25 lung tumors of different histologic types, we found that three MAbs commonly used for their specificities for lymphoid markers were highly reactive with non-small-cell carcinomas (NSCLC) and totally nonreactive with small-cell carcinomas (SCLC). Immunostaining was performed by the standard streptavidin-biotin-peroxidase method after microwave antigen retrieval on formalin-fixed, paraffin-embedded tissue sections. LN2 (CD74), LN3 (HLA-DR), and BLA-36, which are commonly used for the identification of B-lymphocytes, strongly immunostained 19 of 25 squamous and adenocarcinomas and none of 34 small-cell carcinomas and carcinoids. Moreover, in combined tumors, these MAbs selectively stained the adenocarcinoma cells but not the adjacent small-cell carcinoma cells. A cocktail mixture of LN2, LN3, and BLA-36 assayed on 24 additional lung tumors produced similar results with even stronger and sharper stainings. Other lymphoid MAbs showed some selective staining but to a lesser degree. Among nonlymphoid MAbs, the results were as expected, with MAbs for cytokeratin (B72.3) and epithelial membrane antigen staining NSCLC but also some SCLC. The MAbs for chromogranin and neuron-specific enolase were not entirely specific, whereas some nerve-cell adhesion molecule MAbs showed good specificity for SCLC. In a field with few specific MAbs, the newly discovered ability of these lymphoid MAbs to discriminate between SCLC and NSCLC may prove useful in the immunohistochemical diagnosis of lung tumors.
Evaluation of Off-State Current Characteristics of Transistor Using Oxide Semiconductor Material, Indium–Gallium–Zinc Oxide (2012)

We measured a significantly low off-state current (135 yA/µm at 85 °C) of a metal oxide semiconductor (MOS) transistor using indium–gallium–zinc oxide (IGZO), which is an oxide semiconductor material. Note that “y” is 10 -24 . A transistor in which the hydrogen concentration in an IGZO film is lowered (5×10 19 cm -3 or lower) was used. To estimate the minute current accurately, we established a measurement method in which changes in the amount of electrical charge are measured for a long time. Such extremely low off-state current characteristics show promise for new applications of IGZO transistors in memories.
Contribution of Renal Innervation to Hypertension in Rat Autosomal Dominant Polycystic Kidney Disease (2008)

The kidney has both afferent (sensory) and efferent (sympathetic) nerves that can influence renal function. Renal innervation has been shown to play a role in the pathogenesis of many forms of hypertension. Hypertension and flank pain are common clinical manifestations of autosomal dominant (AD) polycystic kidney disease (PKD). We hypothesize that renal innervation contributes to the hypertension and progression of cystic change in rodent PKD. In the present study, the contribution of renal innervation to hypertension and progression of renal histopathology and dysfunction was assessed in male Han:SPRD-Cy/+ rats with ADPKD. At 4 weeks of age, male offspring from crosses of heterozygotes (Cy/+) were randomized into either 1) bilateral surgical renal denervation, 2) surgical sham denervation control, or 3) nonoperated control groups. A midline laparotomy was performed to allow the renal denervation (i.e., physical stripping of the nerves and painting the artery with phenol/alcohol). Blood pressure (tail cuff method), renal function (BUN) and histology were assessed at 8 weeks of age. Bilateral renal denervation reduced the cystic kidney size, cyst volume density, systolic blood pressure, and improved renal function (BUN) as compared with nonoperated controls. Operated control cystic rats had kidney weights, cyst volume densities, systolic blood pressures, and plasma BUN levels that were intermediate between those in the denervated animals and the nonoperated controls. The denervated group had a reduced systolic blood pressure compared with the operated control animals, indicating that the renal innervations was a major contributor to the hypertension in this model of ADPKD. Renal denervation was efficacious in reducing some pathology, including hypertension, renal enlargement, and cystic pathology. However, sham operation also affected the cystic disease but to a lesser extent. We hypothesize that the amelioration of hypertension in Cy/+ rats was due to the effects of renal denervation on the renin angiotensin system.
Influence of stimulation parameters on auditory stimulus processing in schizophrenia and major depression: an auditory evoked potential study (1990)

The influence of stimulation frequency and stimulus intensity on the auditory evoked potential components N1 and P2 was investigated in schizophrenic and major depressive patients. The findings in the patients were compared with those in normal controls. At a high stimulation frequency the amplitude of N1 was enhanced in both schizophrenic and major depressive patients; the latency of N1 increased only in the schizophrenic patients. These changes may be related to impairments of auditory input control and processing in these diseases. In the schizophrenic patients, P2 latency was prolonged under treatment with high-potency neuroleptic drugs.
Supported gold nanoparticles as catalysts for organic reactions (2008)

This critical review is intended to attract the interest of organic chemists and researchers on green and sustainable chemistry on the catalytic activity of supported gold nanoparticles in organic transformations. In the general part of this critical review, emphasis is given to the different procedures to form supported gold nanoparticles and to the importance of the support cooperating in the catalysis. Also the convergence of homogeneous and heterogeneous catalysis in the study of gold nanoparticles has been discussed. The core part of this review is constituted by sections in which the reactions catalyzed by supported gold nanoparticles are described. Special emphasis is made on the unique ability of gold catalysts to promote additions to multiple C-C bonds, benzannulations and alcohol oxidation by oxygen (282 references).