gebhart/patrepeval huggingface dataset:
Gender Differences in Acute CNS Trauma and Stroke: Neuroprotective Effects of Estrogen and Progesterone (2000)

Increasing evidence has demonstrated striking sex differences in the pathophysiology of and outcome after acute neurological injury. Lesser susceptibility to postischemic and posttraumatic brain injury in females has been observed in experimental models. Additional evidence suggests this sex difference extends to humans as well. The greater neuroprotection afforded to females is likely due to the effects of circulating estrogens and progestins. In fact, exogenous administration of both hormones has been shown to improve outcome after cerebral ischemia and traumatic brain injury in experimental models. The neuroprotection provided by periinjury administration of these hormones extends to males as well. The mechanisms by which estrogen and progesterone provide such neuroprotection are likely multifactorial, and probably depend on the type and severity of injury as well as the type and concentration of hormone present. Both genomic and nongenomic mechanisms may be involved. Estrogen's putative effects include preservation of autoregulatory function, an antioxidant effect, reduction of A beta production and neurotoxicity, reduced excitotoxicity, increased expression of the antiapoptotic factor bcl-2, and activation of mitogen activated protein kinase pathways. It is hypothesized that several of these neuroprotective mechanisms can be linked back to estrogen's ability to act as a potent chemical (i.e., electron-donating) antioxidant. Progesterone, on the other hand, has a membrane stabilizing effect that also serves to reduce the damage caused by lipid peroxidation. In addition, it may also provide neuroprotection by suppressing neuronal hyperexcitability. The following review will discuss experimental and clinical evidence for sex differences in outcome after acute brain trauma and stroke, review the evidence implicating estrogens and progestins as mediators of this neuroprotection following acute neurological injury, and finally, address the specific mechanisms by which these hormones may protect the brain following acute neurological injury.
Hemodynamic and metabolic effects of low-dose vasopressin infusions in vasodilatory septic shock (2001)

Low-dose vasopressin infusions increased mean arterial pressure, systemic vascular resistance, and urine output in patients with vasodilatory septic shock and hyporesponsiveness to catecholamines. The data indicate that low-dose vasopressin infusions may be useful in treating hypotension in these patients.
Metabolic syndrome: dietary interventions (2004)

It is imperative to identify strategies that favorably impact this deadly syndrome. Here the authors review how different nutrients in food can modify metabolic syndrome, potentially altering its natural history, and related consequences. Although there is no "all-inclusive" diet yet, it seems plausible that a Mediterranean-style diet exhibits most of the desired attributes.
Lactone and Lactam Library Synthesis by Silver Ion-Assisted Orthogonal Cyclization of Unprotected Peptides (1999)

An orthogonal cyclization strategy has been developed to prepare cyclic peptides individually or as a mixture in aqueous solutions. In this strategy, we propose a dual activation by entropy and enthalpy through Ag+ ion coordination of the reactive ends of an unprotected peptide thioester to permit long-range acyl migration. As a result, both lactamization and lactonization of linear peptides can be realized simultaneously or selectively by controlling the reaction conditions. At pH 4, lactonization is favored due to the strong protonation of the amino moieties. However, at pH 5−5.7 lactamization of α-amine of the N-terminal amino acid is favored, particularly with DMSO as a cosolvent. At pH > 6, lactamization of the ε-amine of lysine becomes significant. Furthermore, the Ag+ ion complexation with the peptide thioester precursor in aqueous buffered solutions may facilitate entropy-favored ring−chain tautomerization, which in turn promotes formation of monomeric cyclic products and thereby reduces unwanted oligomerization. Evidence supporting Ag+ ion-directed ring−chain tautomerization includes product distribution profiles, the concentration-independent nature of the cyclization, and lack of competition by intermolecular coupling with other unprotected peptide segments. The N-terminal amino acid has been found to exert a profound effect in conferring high specificity toward end-to-end cyclization. Examination of 23 linear peptide precursors containing different Nα-amino acids showed that the probability of lactamization is almost 20-fold greater in α-amines than ε-amines with N-terminal Gly, Asn, Asp, and Ser. Unprotected linear peptides ranging from 5 to 16 amino acid residues were cyclized individually or as a mixture with good to excellent yields. More importantly, this strategy paves the way for a new approach to synthesizing cyclic peptide libraries in which unprotected peptides are cyclized and released from the resin at the same time. The obtained libraries can, therefore, be used directly for bioassays without further chemical manipulations. This method is also useful for the synthesis of bicyclic peptides containing both sulfur−sulfur and lactam linkages.
Altered mRNA Splicing and Inhibition of Human E-selectin Expression by an Antisense Oligonucleotide in Human Umbilical Vein Endothelial Cells (1996)

We have characterized the mechanism of action of an antisense oligodeoxynucleotide (ASO) targeting human endothelial leukocyte adhesion molecule, E-selectin. ISIS 4730, a 20-base ASO designed to be complementary to a region in the 3'-untranslated region (3'-UTR) of human E-selectin, is a potent and specific inhibitor of both mRNA and protein expression in human umbilical vein endothelial cells. Following treatment with ISIS 4730, a lower molecular weight mRNA (3300 bases) species was detected by Northern blot analysis with a corresponding decrease in the mature E-selectin transcript (3875 bases). The ASO-induced low molecular weight mRNA is stable and remains in the nucleus. We demonstrate that ISIS 4730 targets E-selectin pre-mRNA in the nucleus and promotes cleavage of the pre-mRNA at the hybridization site, resulting in prevention of splicing of the last intron. The change in molecular weight of the E-selectin transcript is the result of loss of the 3'-UTR due to ASO-mediated RNA cleavage and retention of the last intron. Cleavage of the E-selectin pre-mRNA appears to be due to endogenous RNase H or a related enzyme activity.
A vertically integrated GaAs bipolar DRAM cell (1991)

Bipolar DRAM cells consisting of an n-p-n homojunction bipolar transistor integrated vertically over a p-n junction storage capacitor was fabricated and characterized. The floating collector of the access transistor was merged with the n-region of the storage capacitor. The n-type emitter was connected to the bit line and the p-type base was connected to the word line; the p-type substrate served as the ground plate of the storage capacitor. Storage times of up to 8 min were observed in these cells at room temperature. With a half-bandgap activation energy, these cells should have adequate (>100 ms) storage times up to 125 degrees C. Electrical writing was confirmed by monitoring the capacitance of the storage capacitor while write pulses were applied to the word line.
Amelioration of Vascular Dysfunctions in Diabetic Rats by an Oral PKC β Inhibitor (1996)

The vascular complications of diabetes mellitus have been correlated with enhanced activation of protein kinase C (PKC). LY333531, a specific inhibitor of the beta isoform of PKC, was synthesized and was shown to be a competitive reversible inhibitor of PKC beta 1 and beta 2, with a half-maximal inhibitory constant of approximately 5 nM; this value was one-fiftieth of that for other PKC isoenzymes and one-thousandth of that for non-PKC kinases. When administered orally, LY333531 ameliorated the glomerular filtration rate, albumin excretion rate, and retinal circulation in diabetic rats in a dose-responsive manner, in parallel with its inhibition of PKC activities.
High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli (2020)

Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (ΔCAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.
Heterogeneity in late-onset metachromatic leukodystrophy. Effect of inhibitors of cysteine proteinases. (1986)

The synthesis of arylsulfatase A polypeptides was followed in fibroblasts from 11 patients with late-onset forms of metachromatic leukodystrophy. In 10 cell lines, the apparent rate of synthesis was 20%-70% as measured by the amount of [35S]arylsulfatase A secreted in the presence of 10 mM NH4Cl. The specific activity of the secreted arylsulfatase A was normal. The residual activity of arylsulfatase A was below 10% except for one cell line in which it was 20%. The activity of arylsulfatase A and the degradation of sulfatides was partially restored in these fibroblast lines by treatment with irreversible (peptidyl diazomethyl ketones) or competitive (leupeptin) inhibitors of cysteine proteinases. Thus, the mutation(s) in these cell lines led to the synthesis of arylsulfatase. A polypeptides with increased susceptibility to cysteine proteinases. Multiple allelic mutations within this group of late-onset metachromatic leukodystrophy were suggested by the clinical heterogeneity, the variability of the residual activity, and in the response to inhibitors of cysteine proteinases. In fibroblasts from one patient, the apparent rate of synthesis of arylsulfatase A was less than 5%. Furthermore, inhibitors of cysteine proteinases were without effect, suggesting that the mutation in this patient is different from the others.
The Binding Protein for Globular Heads of Complement C1q, gC1qR (1996)

A binding protein for the globular head domains of complement component C1q, designated gC1qR, recently described to be present on vascular and blood cells (Ghebrehiwet, B., Lim, B.-L., Peerschke, E. I. B., Willis, A. C., and Reid, K. B. M. (1994) J. Exp. Med. 179, 1809-1821 was expressed in recombinant form in bacteria to investigate its functional and structural properties. The recombinant gC1qR was found to be functional because tetramerization of the 24.3-kDa polypeptide occurred as described for the native protein, and the binding of the ligand C1q by recombinant gC1qR was indistinguishable from binding shown by gC1qR isolated from Raji cells. Recombinant gC1qR immobilized to microspheres was used to search for additional binding proteins unrelated to C1q. Surprisingly, it was found that vitronectin or complexes containing vitronectin were retained from plasma or serum, and subsequent analysis revealed the specific binding of the ternary vitronectin-thrombin-antithrombin complex to gC1qR. Because the thrombin-antithrombin complex was unable to interact with gC1qR, direct binding with vitronectin was investigated in a purified system. The heparin binding multimeric form of vitronectin but not the plasma form of vitronectin was found to bind specifically to gC1qR isolated from Raji cell membrane as well as to recombinant gC1qR. This interaction was saturable (KD approximately 20 nM) and inhibitable by glycosaminoglycans such as heparin but not by chondroitin sulfate. C1q and vitronectin did not compete with each other for binding to gC1qR, and both ligands seem to interact with different parts of the gC1qR because a truncated version of recombinant gC1qR lacking the N-terminal 22-amino acid portion hardly interacted with vitronectin but bound C1q as well as the intact gC1qR. These findings establish gC1qR as a novel vitronectin-binding protein that may participate in the clearance of vitronectin-containing complexes or opsonized particles or cooperate with vitronectin in the inhibition of complement-mediated cytolysis.
Technical feasibility of using RU-43 fuel in the CANDU-6 reactors of the Cernavoda NPP (2008)

Abstract Recovered uranium (RU) is a by-product of many light-water reactor (LWR) fuel recycling programs. A fissile content in the RU of 0.9 to 1.0 % makes it impossible for reuse in an LWR without re-enrichment, but CANDU reactors have a sufficiently high neutron economy to use RU as fuel. The Institute for Nuclear Research (INR) Pitesti has analyzed the feasibility of using RU fuel with 0.9 – 1.1 w% 235 U in the CANDU-6 reactors of the Cernavoda Nuclear Power Plant (Cernavoda NPP). Using RU fuel would produce a significant increase in the fuel discharge burnup, from 170 MWh/kgU currently achieves with natural-uranium (NU) fuel to about 355 MWh/kgU. This would lead to reduced fuel-cycle cost and a large reduction in spent-fuel volume per full-power-year of operation. The RU fuel bundle design with recovered uranium fuel, known as RU-43, is being developed by the INR Pitesti and is now at the stage of final design verification. Early work has been concentrated on RU-43 fuel bundle design optimization, safety and reactor physics assessment. The changes in fuel element and fuel bundle design contribute to the many advantages offered by the RU-43 bundle. Verification of the design of the RU-43 fuel bundle is performed in a way that shows that design criteria are met, and is mostly covered by proof tests such as flow and irradiation tests. The most relevant calculations performed on this fuel bundle design version are presented. Also, the stages of an experimental program aiming to verify the operating performance are briefly described in this paper.
GenBank: update (2003)

GenBank is a comprehensive database that contains publicly available DNA sequences for more than 140 000 named organisms, obtained primarily through submissions from individual laboratories and batch submissions from large-scale sequencing projects. Most submissions are made using the BankIt (web) or Sequin program and accession numbers are assigned by GenBank staff upon receipt. Daily data exchange with the EMBL Data Library in the UK and the DNA Data Bank of Japan helps ensure worldwide coverage. GenBank is accessible through NCBI's retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome mapping, protein structure and domain information, and the biomedical journal literature via PubMed. BLAST provides sequence similarity searches of GenBank and other sequence databases. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. To access GenBank and its related retrieval and analysis services, go to the NCBI home page at: http://www.ncbi.nlm.nih.gov.
Revised structure for the product from the reaction of 3-hydrazinocarbonylmethylene-2-oxo-1,2,3,4-tetrahydroquinoxaline with nitrous acid. (1984)

The structure of the compound 2, 2, 4-dioxo-3-nitroso-1, 2, 4, 5-tetrahydropyrazolo [1, 5-a]-quinoxaline, which was previously obtained from the reaction of 3-hydrazinocarbonyl-methylene-2-oxo-1, 2, 3, 4-tetrahydroquinoxaline (1a) with an excess of nitrous acid, was revised to 3-(1, 2, 4-oxadiazolin-5-on-3-yl)-2-oxo-1, 2-dihydroquinoxaline (7) on the basis of comparison with an authentic sample prepared from 3-cyano-2-oxo-1, 2-dihydroquinoxaline (8) via an unambiguous route.
Improved, Acid-catalyzed Iodinating Procedures for Activated Aromatics with (Diacetoxyiodo)benzene as the Oxidant (1999)

Activated aromatics are effectively converted, at room temperature and within 15min, into the corresponding mono-, di- or tri-iodinated products in anhydrous l 2 -Phl(OAc) 2 -AcOH-Ac 2 O systems, acidified with catalytic amounts of conc. H 2 SO 4 .
Formation and hydrolysis of maltohexaose by an extracellular exo-maltohexaohydrolase. (1983)

An extracellular exo-maltohexaohydrolase [EC 3.2.1.98] from a Klebsiella pneumoniae (Aerobacter aerogenes) mutant produced about 40% maltohexaose (G6) from short-chain amylose (DP=23). Mostly G6 was produced from maltooligosaccharides larger than G6 by an exomechanism action. It also hydrolyzed G6 and shorter maltooligosaccharides to give smaller maltooligosaccharides. Its position specificity of action on G3 through G8 was studied with maltodextrins specifically labeled at the reducing-end glucose unit with 14C. The highest frequency of cleavage was at the second bond from the reducing end in G3 through G6. For G7 and G8, the sixth bond from the nonreducing end of the substrate was cleaved with absolute specificity by the exo-mechanism action. Kinetic parameters of the exo-maltohexaohydrolase on various substrates were also studied. The Michaelis constant (Km)for short-chain amylose was the smallest among the various substrates examined. G6 was also formed from G4 by a transfer action of the enzyme, with an action pattern dependent on the substrate concentration.
Building transcribed speech corpora quickly and cheaply for many languages (2010)

We present a system for quickly and cheaply building transcribed speech corpora containing utterances from many speakers in a variety of acoustic conditions. The system consists of a client application running on an Android mobile device with an intermittent Internet connection to a server. The client application collects demographic information about the speaker, fetches textual prompts from the server for the speaker to read, records the speaker’s voice, and uploads the audio and associated metadata to the server. The system has so far been used to collect over 3000 hours of transcribed audio in 17 languages around the world. Index Terms: speech corpora, speech recognition, internationalization
Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene. (1991)

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Genomic clones encoding the VCAM1 gene were isolated and the organization of the gene was determined. The gene, which is present in a single copy in the human genome, contains 9 exons spanning approximately 25 kilobases of DNA. Exons 2-8 contain C2 or H-type immunoglobulin domains. At least two different VCAM-1 precursors can be generated from the human gene as a result of alternative mRNA splicing events, which include or exclude exon 5. A consensus TATAA element is located upstream of the transcriptional start site. The VCAM1 promoter contains consensus binding sites for NF-kappa B, the GATA family of transcription factors, as well as an AP1 site. The VCAM1 gene was assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. Structural analysis of the human VCAM1 gene provides the basis for alternative mRNA splicing and an initial approach to elucidating the regulation of VCAM-1 expression.
Lack of effect of anti-hypertensive treatment with felodipine on cardiovascular structure of young spontaneously hypertensive rats (1985)

Felodipine (4(2,3-dichlorophenyl)-1,4-dihydro-2, 6-dimethyl-3-ethoxycarbonyl-5-methoxycarbonyl pyridine)), a selective vasodilating anti-hypertensive drug, was used in the treatment of spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto rats (WKYs) from age 6 to 14 weeks, ie during the time of high blood pressure development in SHRs. The effect of treatment on heart weight and on mesenteric resistance vessels (i.d. ca 170 microns) characteristics was investigated. In a first study, two oral doses of felodipine were added to the diet of SHRs, in concentrations of either 0.5 or 1.5 mg X g-1 rat food. Both treatments lowered mean arterial pressure by about 19% (p less than 0.001). In a second study, the lower dose of felodipine (0.5 mg . g-1 rat food) was therefore used to treat both SHRs and WKYs. Treatment did not interfere with weight or food intake of either SHRs or WKYs but increased average weekly water intake significantly. In neither strain was the pulse rate or, surprisingly, heart/body weight ratio affected by treatment. Furthermore, mesenteric resistance vessel morphology and mechanics were not affected by the blood pressure reduction. The noradrenaline and calcium sensitivity of mesenteric resistance vessels from treated rats was greater (p less than 0.001) than those from control rats. These findings indicate that blood pressure reduction with felodipine does not affect cardiovascular structure in young SHRs.
Increased light extraction efficiency from top-emitting organic light-emitting diodes employing a mask-free plasma-etched stochastic polymer surface (2016)

Top-emitting organic light-emitting diodes (TEOLEDs) are drawing interest as future devices for both high-quality display and lighting. However, the current TEOLEDs have external quantum efficiencies of approximately 20%, which still need improvement. To attain high device efficiencies in TEOLEDs, waveguide and surface plasmon polariton modes should be minimised. Many efforts have been made using nano- or micrometre-scale periodic gratings to extract the confined photons. However, significant angular distortion or colour shifts occur as the Bragg condition calls for. Here, an effective method is demonstrated for enhancing the light extraction efficiency from TEOLEDs employing a mask-free plasma-etched polymer surface with stochastically distributed nano-hemispheres. TEOLEDs with the stochastic polymer surface allowed us to achieve a device efficiency enhancement up to 1.55-fold relative to conventional devices without introducing spectral changes and angular emission distortion, unlike periodic grating-embedded devices. Because of the independence of the improvement from the particular emission wavelengths, the proposed TEOLEDs are attractive and practical for use in full colour and white lighting as well as display applications.
Cutting Edge: Paradigm Revisited: Antibody Provides Resistance to Listeria Infection (1999)

Listeriolysin O (LLO) is a secreted pore-forming toxin of the facultative intracellular bacterium Listeria monocytogenes. We assessed the ability of a murine anti-LLO mAb to affect the course of infection in mice challenged with Listeria. This mAb was previously shown to be capable of neutralizing LLO-mediated pore formation in vitro, and here we show that the passive administration of this Ab to mice before infection provides increased resistance. Mice treated with the mAb were protected from a lethal challenge with virulent Listeria and showed a significant reduction in Listeria burden during the first hours to days postinfection. These effects of the Ab were independent of host B or T cells, since treatment with the mAb provided enhanced resistance to SCID mice. The titer of anti-LLO Abs during the regular infection of mice with Listeria was found to be low to negative.