gebhart/patrepeval huggingface dataset:
FACILE IMAGE-BASED CELL SORTING USING OPTO- FLUCS (OPTO-FLUIDIC CELL SORTING) (2006)

We present a new approach for sorting cells based upon visual information, termed opto-fluidic cell sorting (OPTO-FluCS). This technique uses a combination of hydrodynamic and optical scattering forces to array, image, and then sort many individual cells. Our device combines the strengths of microscopy–imaging in space and time–with the ability to array cell populations and isolate cells of interest in an intuitive, user-friendly fashion. We successfully demonstrate image-based sorting based on fluorescence localization, a sort unavailable with flow-assisted cell sorting.
Diversity and Complexity of the Mu Opioid Receptor Gene: Alternative Pre-mRNA Splicing and Promoters (2005)

Mu opioid receptors play an important role in mediating the actions of a class of opioids including morphine and heroin. Binding and pharmacological studies have proposed several mu opioid receptor subtypes: mu(1), mu(2), and morphine-6beta-glucuronide (M6G). The cloning of a mu opioid receptor, MOR-1, has provided an invaluable tool to explore pharmacological and physiological functions of mu opioid receptors at the molecular level. However, only one mu opioid receptor (Oprm) gene has been isolated. Alternative pre-mRNA splicing has been proposed as a molecular explanation for the existence of pharmacologically identified subtypes. In recent years, we have extensively investigated alternative splicing of the Oprm gene, particularly of the mouse Oprm gene. So far we have identified 25 splice variants from the mouse Oprm gene, which are controlled by two diverse promoters, eight splice variants from the rat Oprm gene, and 11 splice variants from the human Oprm gene. Diversity and complexity of the Oprm gene was further demonstrated by functional differences in agonist-induced G protein activation, adenylyl cyclase activity, and receptor internalization among carboxyl terminal variants. This review summarizes these recent results and provides a new perspective on understanding and exploring complex opioid actions in animals and humans.
A Targeted Deletion of a Region Upstream from the Jκ Cluster Impairs κ Chain Rearrangement In Cis in Mice and in the 103/bcl2 Cell Line (1999)

We have shown previously that a mutation of the KI-KII site immediately 5' to J(kappa)1 on the mouse immunoglobulin light chain kappa locus reduces the rearrangement level in cis, although it does not affect transcription. Here we deleted by homologous recombination in mouse embryonic stem cells a 4-kb DNA fragment, located immediately upstream of the KI-KII element, which contains the promoter of the long germline transcript. Analysis of gene-targeted heterozygous mouse splenic B cells showed a strong decrease in rearrangement for the allele bearing the deletion. When both the KI-KII mutation and the 4-kb deletion were present on the same allele, the overall reduction in rearrangement was stronger than with the 4-kb deletion alone underlying the role of these two elements in the regulation of rearrangement. The same deletion was performed by homologous recombination on one allele of the rearrangement-inducible mouse 103/bcl2-hygro(R) pre-B cell line, and resulted in a similar reduction in the induction of rearrangement of the mutated allele. This result validates this cell line as an in vitro model for studying the incidence of gene-targeted modifications of the kappa locus on the regulation of rearrangement.
The PCSK9 decade (2012)

PCSK9 proprotein convertase subtilisin/kexin type (PCSK9) is a crucial protein in LDL cholesterol (LDL-C) metabolism by virtue of its pivotal role in the degradation of the LDL receptor. In recent years, both in vitro and in vivo studies have greatly supplemented our understanding of the (patho)physiological role of PCSK9 in human biology. In the current review, we summarize studies published or in print before May 2012 concerning the physiological role of PCSK9 in cholesterol metabolism. Moreover, we briefly describe the clinical phenotypes encountered in carriers of mutations in the gene encoding PCSK9. As PCSK9 has emerged as a novel target for LDL-C lowering therapy, methods to inhibit PCSK9 will also be reviewed. Initial data from investigations of PCSK9 inhibition in humans are promising and indicate that PCSK9 inhibition may be a viable new therapeutic option for the treatment of dyslipidemia and associated cardiovascular diseases.
Discrete model of fish scale incremental pattern: a formalization of the 2D anisotropic structure (2004)

Abstract The structure of growth patterns on fish scales is characteristically anisotropic: the number of circuli and their widths significantly vary with the direction of measurement. We show, however, that because of anisotropy, fish scale growth rate variability can be described in fuzzy terms. The index of structural anisotropy is introduced, which serves as a measure of the fuzziness of growth-rate quantification. A discrete model of fish scale incremental pattern is proposed, which takes into account the incremental structure in 2D. This model is based on a representation of the fish scale pattern as a relay network, taking anisotropy in the form of discontinuities and convergences of incremental structural elements into account, and the widths of growth increments in different directions. The model is used to formalize procedures necessary for the quantification of fish scale growth rate. The capability of the model for analysing objects with similar structural attributes as found in fish scale incremental patterns, such as those found in coral, otoliths, shells, and bones, is demonstrated.
Purification and characterization of prolixin S (nitrophorin 2), the salivary anticoagulant of the blood-sucking bug Rhodnius prolixus (1995)

The salivary anticoagulant of the blood-sucking bug Rhodnius prolixus was purified to homogeneity using a protocol consisting of weak cation-exchange, DEAE, hydrophobic-interaction and octadecyl reverse-phase chromatography, yielding a protein with the same N-terminal sequence as nitrophorin 2, one of the four NO haem protein carriers present in the salivary glands of Rhodnius with a molecular mass of 19689 Da [D. Champagne, R.H. Nussenzveig and J.M.C. Ribeiro, (1995) J. Biol. Chem. 270, in the press]. To exclude the possibility of the nitrophorin being a contaminant, another chromatographic protocol was performed, consisting of chromatofocusing followed by strong-cation-exchange chromatography. Again the anticoagulant was eluted with nitrophorin 2. Nitrophorin 2 inhibits coagulation Factor VIII-mediated activation of Factor X and accounts for all the anti-clotting activity observed in Rhodnius salivary glands.
Resolution limit in community detection (2006)

Detecting community structure is fundamental for uncovering the links between structure and function in complex networks and for practical applications in many disciplines such as biology and sociology. A popular method now widely used relies on the optimization of a quantity called modularity, which is a quality index for a partition of a network into communities. We find that modularity optimization may fail to identify modules smaller than a scale which depends on the total size of the network and on the degree of interconnectedness of the modules, even in cases where modules are unambiguously defined. This finding is confirmed through several examples, both in artificial and in real social, biological, and technological networks, where we show that modularity optimization indeed does not resolve a large number of modules. A check of the modules obtained through modularity optimization is thus necessary, and we provide here key elements for the assessment of the reliability of this community detection method.
Comparison of aldehyde-producing activities of cyanobacterial acyl-(acyl carrier protein) reductases (2016)

We found that the activity, substrate specificity, and solubility are diverse among various AARs. Our results provide a basis for selecting an AAR sequence suitable for metabolic engineering of bioalkane production while regulating carbon chain length.
Ultrathin Alumina Mask-Assisted Nanopore Patterning on Monolayer MoS2 for Highly Catalytic Efficiency in Hydrogen Evolution Reaction (2018)

Nanostructured molybdenum disulfide (MoS<sub>2</sub>) has been considered as one of the most promising catalysts in the hydrogen evolution reaction (HER), for its approximately intermediate hydrogen binding free energy to noble metals and much lower cost. The catalytically active sites of MoS<sub>2</sub> are along the edges, whereas thermodynamically MoS<sub>2</sub> favors the presence of a two-dimensional (2-D) basal plane and the catalytically active atoms only constitute a small portion of the material. The lack of catalytically active sites and low catalytic efficiency impede its massive application. To address the issue, we have activated the basal plane of monolayer 2H MoS<sub>2</sub> through an ultrathin alumina mask (UTAM)-assisted nanopore arrays patterning, creating a high edge density. The introduced catalytically active sites are identified by Cu electrochemical deposition, and the hydrogen generation properties are assessed in detail. We demonstrate a remarkably improved HER performance as well as the identical catalysis of the artificial edges and the pristine metallic edges of monolayer MoS<sub>2</sub>. Such a porous monolayer nanostructure can achieve a much higher edge atom ratio than the pristine monolayer MoS<sub>2</sub> flakes, which can lead to a much improved catalytic efficiency. This controllable edge engineering can also be extended to the basal plane modifications of other 2-D materials, for improving their edge-related properties.
Realistic power-amplifiers characterization with application to baseband digital predistortion for 3G base stations (2002)

In this paper, a realistic, accurate, versatile, and thermal-free complex behavior test bed suitable for third-generation power-amplifiers characterization is proposed. Using this approach, a 90-W peak power amplifier based on Motorola-LDMOS class-AB transistors was measured under several signal excitations such as W-CDMA, cdma2000, and eight-tone signals. The results obtained show noticeable discrepancies compared to those measured using a vector network analyzer (HP-8510C) for both AM/AM and AM/PM curves. This test bed was also used for the investigation of the memory effect in RF power amplifiers. In the second part of this paper, the characterization results obtained by the test bed were used to design a digital predistorter for an LDMOS amplifier. A baseband predistortion accurate synthesis algorithm is presented. Indeed, a memoryless baseband digital predistorter lookup table was directly synthesized using the measured AM/AM and AM/PM curves without any need to perform additional analytical derivations and/or numerical optimizations. The predistorter synthesis procedure requires only one iteration, contrary to previous works, which need several iterations to obtain similar performances.
Catalytic Properties and Structural Components of Lysyl Oxidase (2007)

Key aspects of the biosynthesis and catalytic specificity of lysyl oxidase (LO) have been explored. Oxidation of peptidyl lysine in synthetic oligopeptides is markedly sensitive to the presence of vicinal dicarboxylic ami/no acid residues. Optimal activity is obtained with the -Glu-Lys- sequence within a polyglycine 11-mer, whereas the -Lys-Glu- sequence is much less efficiently oxidized. The -Asp-Glu-Lys- sequence is a very poor substrate, although this sequence is oxidized in type I collagen fibrils. These results are considered in the light of a model requiring collagen to be assembled as fibrils prior to oxidation by LO. An in vitro system for the expression of catalytically active LO has been devised. Deletion or inclusion of the cDNA coding for the propeptide region in the expressed construct results in apparently identical, catalytically active enzyme products, indicating the lack of essentiality of this region for active enzyme production. These effects are considered with respect to the conservation of the amino acid sequence of LO produced by different species.
Regulation by Chemokines of Circulating Dendritic Cell Precursors, and the Formation of Portal Tract–Associated Lymphoid Tissue, in a Granulomatous Liver Disease (2000)

We have studied the recruitment and roles of distinct dendritic cell (DC) precursors from the circulation into Propionibacterium acnes-induced granulomas in mouse liver. During infection, F4/80(-)B220(-)CD11c(+) DC precursors appeared in the circulation, migrated into the perisinusoidal space, and matured within newly formed granulomas. Recruited DCs later migrated to the portal area to interact with T cells in what we term "portal tract-associated lymphoid tissue" (PALT). Macrophage inflammatory protein 1alpha attracted blood DC precursors to the sinusoidal granuloma, whereas secondary lymphoid organ chemokine (SLC) attracted mature DCs to the newly identified PALT. Anti-SLC antibody diminished PALT expansion while exacerbating granuloma formation. Therefore, circulating DC precursors can migrate into a solid organ like liver, and participate in the granulomatous reaction in response to specific chemokines.
A Non-Operative Salvage of Surgically-Resistant Pseudarthroses and Non-Unions by Pulsing Electromagnetic Fields (1977)

This report documents, for the first time, to the authors' knowledge, the therapeutic use in humans of low energy, electromagnetic fields pulsing in the extremely low frequency (E.L.F.) range. These fields, established outside the body, were used to treat congenital and acquired pseudarthroses and non-unions. Energy of this type appears to affect biological processes, not through heat production, but through electrically-induced changes in the environment of cells within the organism. Of the 29 patients included in the study, 17 had experienced at least one failure of surgical repair and, in each of these, amputation had been recommended. The overall success rate, including those patients treated with inadequate pulse characteristics and those who failed to follow the protocol, was in excess of 70 per cent. Improvements in the specificity of pulse characteristics hold promise for increasing the rate of success. The simple, clinical methodology, which is conducted on an out-patient basis, appears to be both safe and effective. It can be applied with or without surgery. This approach requires additional controlled investigations before it is ready for general use in the orthopaedic community. The indications for amputation of surgically-resistant pseudarthroses, however, should be reassessed. The principles and technology, which have been established during this endeavor, may have physiologic and practical significance for processes other than pseudarthrosis and non-union.
High frequency of epithelial chimerism in liver transplants demonstrated by microdissection and STR-analysis (2002)

It has recently been shown that epithelial cells derived from stem cells originating outside the liver are integrated into liver allografts. Whether epithelial intragraft chimerism protects transplants from rejection or chronic transplant dysfunction, and whether it interferes with recurrence of primary liver disease, is not known. Twenty-seven sequential biopsies derived from 9 liver-transplant recipients were studied for chimerism of hepatocytes and cholangiocytes. The target cells were isolated by laser microdissection after cytokeratin immunolabeling and genotyped using DNA analysis of a highly polymorphic short tandem repeat. Irrespective of whether early (up to 4 weeks) or late (more than 12 months) posttransplantation biopsies were studied, cholangiocyte chimerism was almost constantly found in 91% of the samples. No significant differences occurred between samples derived from patients with chronic organ dysfunction (n = 3), recurrent hepatitis (n = 3), or mild, unspecific changes (n = 3). By contrast, hepatocyte chimerism tended to occur later (55% vs. 22%) and appeared to be associated with recurrent hepatitis (67% vs. 27%). In this respect, chronic organ dysfunction did not differ from mild, unspecific changes. While cholangiocyte chimerism represents a constant and early phenomenon in liver transplantations, an enhanced chimeric integration of recipient-derived hepatocytes can be observed in recurrent hepatitis, supporting the concept of an increased recruitment of extrahepatic progenitor cells to the liver in chronic hepatitis.
Coordination chemistry of microbial iron transport compounds. 9. Stability constants for catechol models of enterobactin (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCoordination chemistry of microbial iron transport compounds. 9. Stability constants for catechol models of enterobactinAlex Avdeef, Stephen R. Sofen, Thomas L. Bregante, and Kenneth N. RaymondCite this: J. Am. Chem. Soc. 1978, 100, 17, 5362–5370Publication Date (Print):August 1, 1978Publication History Published online1 May 2002Published inissue 1 August 1978https://pubs.acs.org/doi/10.1021/ja00485a018https://doi.org/10.1021/ja00485a018research-articleACS PublicationsRequest reuse permissionsArticle Views2487Altmetric-Citations266LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Vacuum Deposition of Thin Films (1957)

Vacuum deposition of thin films , Vacuum deposition of thin films , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Selective Bromination with Copper(II) Bromide1 (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelective Bromination with Copper(II) Bromide1L. Carroll King and G. Kenneth OstrumCite this: J. Org. Chem. 1964, 29, 12, 3459–3461Publication Date (Print):December 1, 1964Publication History Published online1 May 2002Published inissue 1 December 1964https://pubs.acs.org/doi/10.1021/jo01035a003https://doi.org/10.1021/jo01035a003research-articleACS PublicationsRequest reuse permissionsArticle Views6729Altmetric-Citations247LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Temperature-Programmed Reaction and Desorption of the Sensor Elements of a WO[sub 3]∕YSZ∕Pt Potentiometric Sensor (2006)

Temperature-programmed reaction (TPR) and desorption (TPD) were performed to determine the reaction and adsorption characteristics of , CO, , , and their mixtures on the sensor elements of a /yttria-stabilized-zirconia (YSZ)/Pt potentiometric sensor. The results are discussed, focusing on how sensor performance is influenced by catalytic and adsorptive behavior of the electrode materials. The sensor response is most likely given by a dissymmetry of the decomposition activity between the and Pt electrode. No significant adsorption of and its related molecules is observed during the TPD of and -containing gas mixtures. Enhanced NO response by coexisting CO agrees with the increased NO adsorption by the addition of CO, indicating that the NO response is related to gas adsorption behavior.
Curved Oligophenylenes as Donors in Shape‐Persistent Donor–Acceptor Macrocycles with Solvatofluorochromic Properties (2015)

Many optoelectronic organic materials are based on donor-acceptor (D-A) systems with heteroatom-containing electron donors. Herein, we introduce a new molecular design for all-carbon curved oligoparaphenylenes as donors, which results in the generation of unique shape-persistent D-A macrocycles. Two types of acceptor-inserted cycloparaphenylenes were synthesized. These macrocycles display positive solvatofluorochromic properties owing to their D-A characteristics, which were confirmed by theoretical and electrochemical studies.
Structure of the chromosomal insertion site for pSAM2: functional analysis in Escherichia coli (1998)

The element pSAM2 from Streptomyces ambofaciens integrates into the chromosome through site-specific recombination between the element (attP) and the chromosomal (attB) sites. These regions share an identity segment of 58bp extending from the anti-codon loop through the 3' end of a tRNA(Pro) gene. To facilitate the study of the attB site, the int and xis genes, expressed from an inducible promoter, and attP from pSAM2 were cloned on plasmids in Escherichia coil. Compatible plasmids carrying the different attB regions to be tested were introduced in these E. coli strains. Under these conditions, Int alone could promote site-specific integration; Int and Xis were both required for site-specific excision. This experimental system was used to study the sequences required in attB for efficient site-specific recombination. A 26 bp sequence, centred on the anti-codon loop region and not completely included in the identity segment, retained all the functionality of attB; shorter sequences allowed integration with lower efficiencies. By comparing the 26-bp-long attB with attP, according to the Lambda model, we propose that B and B', C and C' core-type Int binding sites consist of 9 bp imperfect inverted repeats separated by a 5 bp overlap region.
Identification of IgG and Fc-Binding Proteins in Human Seminal Plasma and Sperm (1991)

Human seminal plasma contains two novel soluble proteins capable of binding IgG and Fc, but not Fab. The IgG- and Fc-binding proteins were identified by immunoblotting using IgG of various species, Fc and Fab fragments. Their estimated molecular sizes are 16-kD and 20-kD. The monoclonal antibody (MAb), Leu 11b, raised against Fc gamma RIII interacts with the 16-kD protein, whereas other mAbs (32.2, IV.3, and 3G8) raised against FcR did not. The 16-kD protein is capable of binding IgG of several species (human, mouse, rabbits, and goat), whereas the 20-kD protein interacted only with human IgG-Fc fragment. The 16-kD IgG and the 20-kD Fc-binding proteins were found in the PBS extract of human sperm. Human seminal plasma/sperm contain an IgG- and a Fc-binding protein with estimated Mr of 16 and 20 kD, respectively.