gebhart/patrepeval huggingface dataset:
Induced oleoresin biosynthesis in grand fir as a defense against bark beetles. (1995)

Grand fir (Abies grandis) saplings and derived cell cultures are useful systems for studying the regulation of defensive oleoresinosis in conifers, a process involving both the constitutive accumulation of resin (pitch) in specialized secretory structures and the induced production of monoterpene olefins (turpentine) and diterpene resin acids (rosin) by nonspecialized cells at the site of injury. The pathways and enzymes involved in monoterpene and diterpene resin acid biosynthesis are described, as are the coinduction kinetics following stem injury as determined by resin analysis, enzyme activity measurements, and immunoblotting. The effects of seasonal development, light deprivation, and water stress on constitutive and wound-induced oleoresinosis are reported. Future efforts, including a PCR-based cloning strategy, to define signal transduction in the wound response and the resulting gene activation processes are delineated.
The morphology of Ganoderma lucidum mycelium in a repeated-batch fermentation for exopolysaccharide production (2016)

The morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morphology was disposed to EPS production in the shake flask and bioreactor, respectively. Seven RBF could be carried out in 500 mL flasks, and five repeated batches were performed in a 2 L bioreactor. Under RBF conditions, autolysis of pellet core in the shake flask and shaving off of the outer hairy region in the bioreactor were observed at the later stages of RBF (R4 for the shake flask and R6 for the bioreactor). The proposed strategy showed that the morphology of G. lucidum mycelium can withstand extended fermentation cycles.
DNA replication fidelity inEscherichia coli: a multi-DNA polymerase affair (2012)

High accuracy (fidelity) of DNA replication is important for cells to preserve the genetic identity and to prevent the accumulation of deleterious mutations. The error rate during DNA replication is as low as 10(-9) to 10(-11) errors per base pair. How this low level is achieved is an issue of major interest. This review is concerned with the mechanisms underlying the fidelity of the chromosomal replication in the model system Escherichia coli by DNA polymerase III holoenzyme, with further emphasis on participation of the other, accessory DNA polymerases, of which E. coli contains four (Pols I, II, IV, and V). Detailed genetic analysis of mutation rates revealed that (1) Pol II has an important role as a back-up proofreader for Pol III, (2) Pols IV and V do not normally contribute significantly to replication fidelity, but can readily do so under conditions of elevated expression, (3) participation of Pols IV and V, in contrast to that of Pol II, is specific to the lagging strand, and (4) Pol I also makes a lagging-strand-specific fidelity contribution, limited, however, to the faithful filling of the Okazaki fragment gaps. The fidelity role of the Pol III τ subunit is also reviewed.
The shape space of discrete orthogonal geodesic nets (2018)

Discrete orthogonal geodesic nets (DOGs) are a quad mesh analogue of developable surfaces. In this work we study continuous deformations on these discrete objects. Our main theoretical contribution is the characterization of the shape space of DOGs for a given net connectivity. We show that generally, this space is locally a manifold of a fixed dimension, apart from a set of singularities, implying that DOGs are continuously deformable. Smooth flows can be constructed by a smooth choice of vectors on the manifold's tangent spaces, selected to minimize a desired objective function under a given metric. We show how to compute such vectors by solving a linear system, and we use our findings to devise a geometrically meaningful way to handle singular points. We base our shape space metric on a novel DOG Laplacian operator, which is proved to converge under sampling of an analytical orthogonal geodesic net. We further show how to extend the shape space of DOGs by supporting creases and curved folds and apply the developed tools in an editing system for developable surfaces that supports arbitrary bending, stretching, cutting, (curved) folds, as well as smoothing and subdivision operations.
Study on changes of polyamine levels in mice with the development of U14 cervical cancer (2012)

This study was performed to investigate the possible involvement of polyamines in the development of cervical cancer. The objective of the present study was therefore to find the specific polyamine indicators, which could be used as useful markers for the early determination of cervical cancer. A simple method for the simultaneous determination of plasma concentrations of five polyamines in normal and U14 model mice was developed by using HPLC-MS. The samples were derivatized by benzoyl chloride. The derived polyamines were separated on a C18 column by a gradient elution with methanol-water, and then detected with HPLC-MS. The results showed that all polyamine levels in the U14 model mice were higher than those in normal ones. The cadaverine, putrescine and 1, 3-diaminopropane levels were significantly higher in U14 model mice plasma than those in normal mice plasma, especially the putrescine and 1, 3-diaminopropane (<i>P<</i>0.01). The cadaverine, putrescine and 1, 3-diaminopropane levels were significantly higher in U14 mice model urine than those in normal mice urine, especially the cadaverine and 1, 3-diaminopropane (<i>P<</i>0.01). Putrescine, cadaverine and 1, 3-diaminopropane might be the indicators of the cervical cancer.
Myocardial ischemia associated with high-dose carmustine infusion (1991)

Carmustine (BCNU), a nitrosourea derivative, is an antineoplastic agent used recently for the treatment of human solid tumors and lymphomas in high doses with autologous bone marrow transplantation. Cardiac toxicity related to BCNU has not been described well. Three patients are reported without a history of angina pectoris who had clinical and electrocardiographic evidence of myocardial ischemia during and immediately after BCNU infusion. The incidence, cause, and mechanism of such a side effect are unknown but should be considered in patients receiving high-dose carmustine infusions.
Pregnancy-related mortality from preeclampsia and eclampsia (2001)

The continuing racial disparity in mortality from preeclampsia and eclampsia emphasizes the need to identify those differences that contribute to excess mortality among black women, and to develop specific interventions to reduce mortality from preeclampsia and eclampsia among all women.
Theory and evaluation of human robot interactions (2003)

Human-robot interaction (HRI) for mobile robots is still in its infancy. Most user interactions with robots have been limited to tele-operation capabilities where the most common interface provided to the user has been the video feed from the robotic platform and some way of directing the path of the robot. For mobile robots with semiautonomous capabilities, the user is also provided with a means of setting way points. More importantly, most HRI capabilities have been developed by robotics experts for use by robotics experts. As robots increase in capabilities and are able to perform more tasks in an autonomous manner we need to think about the interactions that humans will have with robots and what software architecture and user interface designs can accommodate the human in-the-loop. We also need to design systems that can be used by domain experts but not robotics experts. This paper outlines a theory of human-robot interaction and proposes the interactions and information needed by both humans and robots for the different levels of interaction, including an evaluation methodology based on situational awareness.
Insulin‐like growth factor I receptor is required for the mitogenic and transforming activities of the platelet‐derived growth factor receptor (1995)

R- cells are 3T3-like cells derived from mouse embryos in which the insulin-like growth factor I (IGF-I) receptor (IGF-IR) genes have been disrupted by targeted homologous recombination. These cells cannot grow in serum-free medium supplemented by the growth factors that sustain the growth of other 3T3 cell lines, and cannot be transformed by oncogenes that easily transform wild type mouse embryo cells. We have used these cells to study the role of the IGF-IR in the growth and transformation of cells overexpressing the platelet-derived growth factor (PDGF)-beta beta receptor. We report that an overexpressed PDGF-beta beta receptor fails to induce mitogenesis or transformation in cells lacking the IGF-IR, while capable of doing so in cells expressing the IGF-IR. We conclude that the ability of the activated PDGF-beta beta receptor to stimulate cell proliferation and transformation requires a functional IGF-IR.
Fixed and Adaptive Predictors for Hybrid Predictive/Transform Coding (1985)

Hybrid predictive/transform coding is studied. The usual formulation is to first apply a unitary transform and then code the transform coefficients with independent DPCM coders, i.e., the prediction is performed in the transform domain. This structure is compared to spatial domain prediction, where a difference signal is formed in the spatial domain and then coded by a transform coder. A linear spatial domain predictor which minimizes the mean square prediction error also minimizes the mean square of each transform coefficient. The two structures are equivalent if the transform domain prediction scheme is extended to a more general predictor. Hence, the structure that gives the easiest implementation can be chosen. The spatial domain structure is preferred for motion compensation and for line interlaced video signals. Interframe hybrid coding experiments are performed on interlaced videophone scenes using an adaptive transform coder. Motion compensation gives a rate reduction of 25-35 percent compared to frame difference prediction with the same mean square error. The subjective advantage is even greater, since the "dirty window" effect is not present with motion compensation. It is important to perform the motion estimation with fractional pel accuracy. Field coding with a switched predictor using previous field in moving areas is an interesting alternative to frame coding with frame difference prediction.
Divergent roles for thyroid hormone receptor β isoforms in the endocrine axis and auditory system (1999)

Thyroid hormone receptors (TRs) modulate various physiological functions in many organ systems. The TR alpha and TR beta isoforms are products of 2 distinct genes, and the beta 1 and beta 2 isoforms are splice variants of the same gene. Whereas TR alpha 1 and TR beta 1 are widely expressed, expression of the TR beta 2 isoform is mainly limited to the pituitary, triiodothyronine-responsive TRH neurons, the developing inner ear, and the retina. Mice with targeted disruption of the entire TR beta locus (TR beta-null) exhibit elevated thyroid hormone levels as a result of abnormal central regulation of thyrotropin, and also develop profound hearing loss. To clarify the contribution of the TR beta 2 isoform to the function of the endocrine and auditory systems in vivo, we have generated mice with targeted disruption of the TR beta 2 isoform. TR beta 2-null mice have preserved expression of the TR alpha and TR beta 1 isoforms. They develop a similar degree of central resistance to thyroid hormone as TR beta-null mice, indicating the important role of TR beta 2 in the regulation of the hypothalamic-pituitary-thyroid axis. Growth hormone gene expression is marginally reduced. In contrast, TR beta 2-null mice exhibit no evidence of hearing impairment, indicating that TR beta 1 and TR beta 2 subserve divergent roles in the regulation of auditory function.
Phase variable desialylation of host proteins that bind to Streptococcus pneumoniae in vivo and protect the airway (2004)

Most clinical isolates of Streptococcus pneumoniae consist of heterogeneous populations of at least two colony phenotypes, opaque and transparent, selected for in the bloodstream and nasopharynx, respectively. Microarray analysis revealed 24 orfs that demonstrated differences in expression greater than twofold between variants of independent strains. Twenty-one of these showed increased expression in the transparent variants, including 11 predicted to be involved in sugar metabolism. A single genomic region contains seven of these loci including the gene that encodes the neuraminidase, NanA. In contrast to previous studies, there was no contribution of NanA to adherence of S. pneumoniae to epithelial cells or colonization in an animal model. However, we observed NanA-dependent desialylation of human airway components that bind to the organism and may mediate bacterial clearance. Targets of desialylation included human lactoferrin, secretory component, and IgA2 that were shown to be present on the surface of the pneumococcus in vivo during pneumococcal pneumonia. The efficiency of desialylation was increased in the transparent variants and enhanced for host proteins binding to the surface of S. pneumoniae. Because deglycosylation affects the function of many host proteins, NanA may contribute to a protease-independent mechanism to modify bound targets and facilitate enhanced survival of the bacterium.
Effect of linker length on DNA-binding affinity, cross-linking efficiency and cytotoxicity of C8-linked pyrrolobenzodiazepine dimers (1992)

An efficient synthesis of a homologous series of C8-linked pyrrolobenzodiazepine dimers is reported; compounds with an odd number of methylenes (n= 3 or 5) in the linker show a higher affinity for DNA, enhanced cross-linking efficiency, and are more cytotoxic compared with compounds with either n= 4 or 6.
Placental Transforming Growth Factor-β Is a Downstream Mediator of the Growth Arrest and Apoptotic Response of Tumor Cells to DNA Damage and p53 Overexpression (2000)

The p53 tumor suppressor gene and members of the transforming growth factor-beta (TGF-beta) superfamily play central roles in signaling cell cycle arrest and apoptosis (programmed cell death) in normal development and differentiation, as well as in carcinogenesis. Here we describe a distantly related member of the TGF-beta superfamily, designated placental TGF-beta (PTGF-beta), that is up-regulated in response to both p53-dependent and -independent apoptotic signaling events arising from DNA damage in human breast cancer cells. PTGF-beta is normally expressed in placenta and at lower levels in kidney, lung, pancreas, and muscle but could not be detected in any tumor cell line studied. The PTGF-beta promoter is activated by p53 and contains two p53 binding site motifs. Functional studies demonstrated that one of these p53 binding sites is essential for p53-mediated PTGF-beta promoter induction and specifically binds recombinant p53 in gel mobility shift assays. PTGF-beta overexpression from a recombinant adenoviral vector (AdPTGF-beta) led to an 80% reduction in MDA-MB-468 breast cancer cell viability and a 50-60% reduction in other human breast cancer cell lines studied, including MCF-7 cells, which are resistant to growth inhibition by recombinant wild-type p53. Like p53, PTGF-beta overexpression was seen to induce both G(1) cell cycle arrest and apoptosis in breast tumor cells. These results provide the first evidence for a direct functional link between p53 and the TGF-beta superfamily and implicate PTGF-beta as an important intercellular mediator of p53 function and the cytostatic effects of radiation and chemotherapeutic cancer agents.
The Role of the Bacterial Membrane Protein ActA in Immunity and Protection Against Listeria monocytogenes (1998)

ActA, an essential virulence factor of Listeria monocytogenes, is an integral membrane protein that is required for intracellular motility, cell-to-cell spread, and rapid dissemination of the bacteria in the infected host. To reveal cytotoxic T cell responses against ActA we introduced a recombinant soluble form of ActA into the MHC class I-processing compartment of APC using a variant of listeriolysin mutated within its immunodominant MHC class I epitope. With this experimental system we demonstrate that T cells are induced against ActA during a sublethal infection with L. monocytogenes. However, adoptively transferred cytotoxic CD8+ T cells specific for ActA did not protect mice against a subsequent challenge with this pathogen. This was due to an inability of APC to present ActA by either MHC class I or class II molecules as long as ActA remained tethered to the surface of intracellular viable bacteria. ActA was only presented when L. monocytogenes were engineered to secrete ActA or when the bacteria were killed by antibiotics during the assay. These findings raise questions on the general use of membrane proteins of pathogens as candidates for subunit vaccines.
RAG-l-Deficient Mice Have No Mature 8 and T Lymphocytes (1992)

Randall S. Johnson,7 Karl Herrup,* Susumu Tonegawa, * and Virginia E. PapaioannouO *Howard Hughes Medical Institute at the Center for Cancer Research and Department of Biology Massachusetts Institute of Technology Cambridge, Massachusetts 02139 tDana-Farber Cancer Institute and Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston, Massachusetts 02115 $Developmental Neurobiology Shriver Center for Mental Retardation 200 Trapelo Road Waltham, Massachusetts 02254 SDepartment of Pathology Tufts University School of Medicine and Veterinary Medicine Boston, Massachusetts 02111 Summary The V(D)J recombination activation gene RAG-1 was isolated on the basis of its ability to activate V(D)J re- combination on an artificial substrate in fibroblasts. This property and the expression pattern in tissues and cell lines indicate that RAG-1 eitheractivatesorcata- lyres the V(D)J recombination reaction of immuno- globulin and T cell receptor genes. We here describe the introduction of a mutation in RAG-1 into the germ- line of mice via gene targeting in embryonic stem cells. RAG-l-deficient mice have small lymphoid organs that do not contain mature Band T lymphocytes. The arrest of B and T cell differentiation occurs at an early stage and correlates with the inability to perform V(D)J re- combination. The immune system of the RAG-1 mutant mice can be described as that of nonleaky scid mice. Although RAG-1 expression has been reported in the central nervous system of the mouse, no obvious neu- roanatomical or behavioral abnormalities have been found in the RAG-l-deficient mice. Introduction The generation of antibody and Tcell receptor (TCR) diver- sity is mediated to a large extent by the somatic DNA rearrangement known as V(D)J recombination, which joins variable (V), diversity (D), and joining (J) gene seg- ments (Tonegawa, 1983). The genes for the antigen recep- tors are assembled through this process exclusively in lymphocytes. V(D)J recombination occurs at seven differ- ent loci: the immunoglobulin (lg) heavy chain,
LOAM: Lidar Odometry and Mapping in Real-time (2014)

We propose a real-time method for odometry and mapping using range measurements from a 2-axis lidar moving in 6-DOF. The problem is hard because the range measurements are received at different times, and errors in motion estimation can cause mis-registration of the resulting point cloud. To date, coherent 3D maps can be built by off-line batch methods, often using loop closure to correct for drift over time. Our method achieves both low-drift and low-computational complexity without the need for high accuracy ranging or inertial measurements. The key idea in obtaining this level of performance is the division of the complex problem of simultaneous localization and mapping, which seeks to optimize a large number of variables simultaneously, by two algorithms. One algorithm performs odometry at a high frequency but low fidelity to estimate velocity of the lidar. Another algorithm runs at a frequency of an order of magnitude lower for fine matching and registration of the point cloud. Combination of the two algorithms allows the method to map in real-time. The method has been evaluated by a large set of experiments as well as on the KITTI odometry benchmark. The results indicate that the method can achieve accuracy at the level of state of the art offline batch methods.
Identification of endosialin, a cell surface glycoprotein of vascular endothelial cells in human cancer. (1992)

Cell surface antigens of transformed cells are the traditional targets for antibody-guided detection and therapy of solid neoplasms. Alternative targets may be found in the tumor stroma, which contains newly formed blood vessels, reactive fibroblasts, and extracellular matrix proteins. The F19 cell surface glycoprotein of reactive fibroblasts is a prototypic stromal antigen since it is found in the stroma of > 90% of common epithelial cancers but is absent or expressed at low levels in normal tissues and benign epithelial tumors. In the present study, we define an additional tumor stromal antigen, FB5, that is selectively expressed in vascular endothelial cells of malignant tumors. Immunohistochemical analysis of 128 tumors identified FB5 in endothelial cells in 67% of the samples (including 41 of 61 sarcomas, 26 of 37 carcinomas, and 18 of 25 neuroectodermal tumors) whereas normal blood vessels and other adult tissues tested lacked FB5 expression. In vitro studies showed that FB5 is a M(r) 165,000 cell surface glycoprotein, comprised of a M(r) 95,000 core polypeptide and highly sialyated O-linked oligosaccharides but few if any N-linked sugars, and that the FB5 gene is located on chromosome 11q13-qter. The restricted tissue distribution of the FB5 protein, which we refer to as endosialin, suggests strategies for tumor imaging and therapy that are aimed primarily at the tumor vasculature.
Antennas and Radio Wave Propagation (1986)

Antennas and radiowave propagation , Antennas and radiowave propagation , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Enzyme Catalysis in Organic Synthesis. A Comprehensive Handbook, Vols. I-III (2002)

Organische Synthese mit Enzymen? Heute ein Muß! Diese völlig überarbeitete und erweiterte zweite Auflage des praxisbewährten Handbuchs liefert leicht zugänglich alle notwendigen Informationen für den erfolgreichen Einsatz von Enzymen in der Organischen Synthese. Für jeden, der sich mit Katalyse und Synthesechemie beschäftigt.