gebhart/patrepeval huggingface dataset:
Further in vivo studies on the role of the molecular chaperone, Hsp93, in plastid protein import (2007)

In Arabidopsis, Hsp93 is encoded by two genes, atHSP93-V and atHSP93-III. We identified two T-DNA mutants for atHSP93-III: one being a partial 'knockdown' (hsp93-III-1) and the other a complete 'knockout' (hsp93-III-2). Homozygotes for both mutants were indistinguishable from wild type. We crossed each mutant to an atHSP93-V knockout, and identified double mutants with strongly chlorotic phenotypes. This implied redundancy, which was confirmed by the complementation of mildly chlorotic hsp93-V plants by atHSP93-III over-expression. While the hsp93-V hsp93-III-1 mutant was doubly homozygous, the second double mutant was heterozygous for hsp93-III-2 (genotype: hsp93-V/hsp93-V; +/hsp93-III-2). Attempts to identify an hsp93-V hsp93-III-2 double homozygote were unsuccessful, indicating that the Hsp93 pool is essential for viability. Consistently, siliques of the second double mutant contained aborted seeds (because of a block in the zygote-embryo transition) and failed ovules (because of a moderate defect in female gametophytes). Double-mutant plants were chlorophyll-deficient, contained under-developed chloroplasts, and exhibited stunted growth. In import assays using a chimeric pre-protein (plastocyanin transit peptide fused to dihydrofolate reductase; PC-DHFR), a clear defect was observed in hsp93-V hsp93-III-1 chloroplasts. Interestingly, while denaturation or stabilization of the DHFR moiety had a strong effect on import efficiency in the wild type, no such effects were observed with double-mutant (or tic40) chloroplasts. This indicated that pre-protein unfolding is not rate-limiting for import into mutant chloroplasts, and suggested that (unlike the situation in mitochondria) the inner membrane import machinery does not contribute to pre-protein unfolding at the organellar surface.
The efficacy of amitraz against cattle ticks in Tanzania. (1996)

The efficacy of amitraz on cattle ticks was assessed by susceptibility tests, spraying and dipping trials. Tests on the susceptibility of three tick species, Rhipicephalus appendiculatus, Amblyomma variegatum and Boophilus decoloratus to amitraz (technical grade-purity 98.6% w/w) showed all 15 tick strains tested to be highly susceptible, with LC50 ranging between 0.001% and 0.03%, and LC99, between 0.01% and 0.07%. Spraying trials were carried out on calves infested with R. appendiculatus, A. variegatum and B. decoloratus. Amitraz wettable powder [as Taktic total replacement (TR)] was tested against B. decoloratus, and amitraz emulsifiable concentrate (as Taktic EC) against the other two species. Both formulations showed instant action, with ticks detaching from the calves between 30 min and 8 h after spraying. More than 50% of the detached engorged females failed to lay eggs. The remainder laid few eggs, and these had a low hatching rate of 0-2%, compared with 90-98% in the controls. The detached nymphs failed to moult, and the males and non-engorged females also detached, were immobilized and finally died. In the dipping trials, cattle heavily infested with ticks (mean tick counts of about 800) were dipped once weekly in amitraz (Taktic TR). Weekly tick counts showed that the reinfestation rate was reduced to zero after the ninth dipping. The results of the three trials complement each other, showing that amitraz is at present effective in the control of African tick species on cattle in Tanzania.
Mechanistic studies on oxidative condensation of a thymidine 3'-H-phosphonate derivative with 3'-O-acetylthymidine (2008)

Oxidation-reduction condensation using triphenylphosphine and 2,2-dipyridildisulfide was carried out for forming a phosphodiester bond between a mono-anionic thymidine 3-Hphosphonate derivative and 3-O-acetylthymidine. This reaction proceeded with simultaneous coupling and oxidation. The detailed NMR analysis of this reaction shows that an S-(2-pyridyl) phosphorothioate diester derivative was formed as an initial intermediate.
Ferritin-bearing lymphocytes in the diagnosis of breast cancer (1984)

Four hundred forty-seven women attending a breast clinic because of either suspicious lesions, anxiety about breast cancer, follow-up after the removal of a benign breast lesion, or a family history of breast cancer had a routine test for percentage of ferritin-bearing lymphocytes ( FBL ) in their peripheral blood. Among patients who received surgery following physical examination in the clinic and/or mammography, the test was positive in 40 of the 45 (89%) with Stage I;II carcinoma, 3 of 3 with Stage IV carcinoma, and only in 29 of the 97 (37%) with benign breast disease. The possible reasons for the poorer detection rate in Stage III carcinoma are discussed. The test, however, identified 2 cases of Stage I carcinoma, 1 of breast lymphoma, and 12 with premalignant lesions in those who were found normal on physical examination and mammography. Ferritin-bearing lymphocyte results tended to become negative after surgical removal of the lesion, and became positive on recurrence of the tumor and appearance of metastases. The detection rate was maximized by combining the FBL test with the clinical modes of detection.
A Feasibility Study of Tissue Characterization Using LC Sensors (2009)

Bio-implantable sensors using radio-frequency telemetry links that enable the continuous monitoring and recording of physiological data are receiving a great deal of attention. The objective of this paper is to study the feasibility of an implantable sensor for tissue characterization. This has been done by querying an LC sensor surrounded by dispersive tissues by an external antenna. The resonant frequency of the sensor is monitored by measuring the input impedance of the antenna, and correlated to the desired quantities. Using an equivalent circuit model of the sensor that accounts for the properties of the encapsulating tissue, analytical expressions have been developed for the extraction of the tissue permittivity and conductivity. Finally, experimental validation has been performed with a telemetry link that consists of a loop antenna and a fabricated LC sensor immersed in single and multiple dispersive phantom materials.
Chemically stable, lipophilic prodrugs of phosphoramide mustard as potential anticancer agents (1991)

Benzyl phosphoramide mustard (3), 2,4-difluorobenzyl phosphoramide mustard (4), and methyl phosphoramide mustard (5) were examined as lipophilic, chemically stable prodrugs of phosphoramide mustard (2). These phosphorodiamidic esters are designed to undergo biotransformation by hepatic microsomal enzymes to produce 2. The rate of formation of alkylating species, viz., 2, from these prodrugs and their in vitro cytotoxicity toward mouse embryo Balb/c 3T3 cells were comparable to or better than that of cyclophosphamide (1). Preliminary antitumor screening against L1210 leukemia in mice, however, suggests that these prodrugs are devoid of any significant antitumor activity in vivo.
Robust time-optimal control of uncertain structural dynamic systems (1993)

A time-optimal open-loop control problem of flexible spacecraft in the presence of modeling uncertainty has been investigated. The results indicate that the proposed approach significantly reduces the residual structural vibrations caused by modeling uncertainty. The results also indicate the importance of proper jet placement for practical tradeoffs among the maneuvering time, fuel consumption, and performance robustness.
Characterization of the receptor for epidermal growth factor-urogastrone in human placenta membranes. (1980)

The binding of mouse epidermal growth factor-urogastrone (EGF-URO) to membranes from term human placenta is peptide-specific, saturable (about 20 pmol of EGF-URO bound maximally/mg of protein), reversible, and of high affinity (KD about 400 pM). Optimal binding is observed at pH 7.6. At low pH (3.5 to 5.0). EGF-URO can be reversibly dissociated from the receptor; however, exposure to pH < 3 irreversibly inactivates the receptor. The binding, which does not exhibit ligand cooperativity, exhibits an association rate constant of 6.1 x 10(-4) s-1 and a dissociation rate constant of 6.1 x 10(-4) s-1. The dissociation constant determined from the rate constants, 240 pM, is in reasonable agreement with the constant estimated by equilibrium methods. Both monovalent and divalent cations augment EGF-URO binding 2- to 3-fold. Although in general, divalent cations enhance binding at lower concentrations (optimum, 5 mM) than do monovalent cations (optimum, approximately 80 mM), there is no cation-specific effect. Neither guanine nor adenine nucleotides affect EGF-URO binding. Whereas the proteolytic enzymes (trypsin, chymotrypsin, papain, and pepsin) inactivate the receptor, neuraminidase and phospholipases A2, C, and D augment EGF-URO binding. Neuraminidase increases the number of available sites without affecting ligand affinity. Wheat germ agglutinin, concanavalin A, and phytohemagglutinin all compete for the binding of EGF-URO. The data complement previous observations of EGF-URO binding obtained in intact cells and provide a basis for the solubilization, characterization, and isolation of this receptor from a rich tissue source.
Growth inhibition of human colon carcinoma cells by combinations of anti-epidermal growth factor-related growth factor antisense oligonucleotides. (1996)

GEO is a well-differentiated colon cancer cell line that coexpresses the epidermal growth factor-like growth factors CRIPTO (CR), amphiregulin (AR), and transforming growth factor alpha (TGF-alpha). Antisense 20-mer phosphorothioate oligodeoxynucleotides (AS S-oligos) directed against CR, AR, and TGF-alpha mRNAs were equipotent in their ability to inhibit both the anchorage-dependent growth and the anchorage-independent growth (AIG) of GEO cells, with a 50% inhibitory concentration of about 5 micrometer in the AIG assay. A supraadditive effect was observed when a combination of S-oligos was used. For example, a combination of two different AS S-oligos (either AR + CR, or TGF-alpha + CR, or TGF-alpha + AR) at a concentration of 1 micrometer each (total concentration, 2 micrometer) resulted in 50% inhibition of GEO cells AIG, whereas the use of each AS S-Oligo at a 1 or 2 micrometer concentration resulted respectively in about 10 and 20% growth inhibition. A combination of the three AS S-oligos was even more effective, resulting in about 60% inhibition of GEO cells AIG at a concentration of 1 micrometer each (3 micrometer total concentration). The AS S-oligos were also able to inhibit specifically the expression of either AR, CR, or TGF-alpha proteins in GEO cells, as assessed using immunocytochemistry or Western blot analysis. Finally, a supraadditive growth inhibitory effect of the AS S-oligos and an epidermal growth factor receptor-blocking antibody (monoclonal antibody 528) was observed. These data suggest that the use of a combination of AS S-oligos directed against different growth factors and antibodies directed against their receptors might result in an efficient inhibition of colon carcinoma cell growth.
E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor (2010)

Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. Heparosan is also the capsular polysaccharide of Escherichia coli K5 strain. The current study was focused on the examination of the fermentation of E. coli K5 with the goal of producing heparosan in high yield and volumetric productivity. The structure and molecular weight properties of this bacterial heparosan were determined using polyacrylamide gel electrophoresis (PAGE) and Fourier transform mass spectrometry. Fermentation of E. coli K5 in a defined medium using exponential fed-batch glucose addition with oxygen enrichment afforded heparosan at 15 g/L having a number average molecular weight of 58,000 Da and a weight average molecular weight of 84,000 Da.
DYNAMIC SLUG TRACKING SIMULATIONS FOR GAS-LIQUID FLOW IN PIPELINES (1996)

Abstract A Lagrangian slug tracking model for dynamic gas-liquid slug flow in pipelines of varying inclinations has been formulated and implemented in C + + using an object-oriented approach. Slugs and bubbles are discrete computational objects which are coupled by exchange of mass and momentum, and organized in linked lists The flow parameters are determined from dynamic integral mass and momentum balances on each slug and bubble. A subgrid is used in bubbles extending over pipe sections with different inclinations Slugs and bubbles are initiated at the pipe inlet, or at low points along a pipeline, and the propagation of individual slugs are tracked dynamically, and without numerical diffusion. Some sample cases demonstrate how the structure of the flow can be followed as it evolves from terrain effects, expansion and wake effects. These are effects that may in some instances cause slugs to disappear and merging of bubbles during simulations. Terrain slugging compulations compare well with some experimental data.
Role of Glycosylation Sites in the IgE Fc Molecule (1995)

The Fc region of immunoglobulin E (IgE) comprising the C epsilon 3 and C epsilon 4 domains (residues 329-547) is sufficient for binding to the high-affinity IgE Fc receptor (Fc epsilon RI alpha). Three potential N-linked glycosylation sites are present within the C epsilon 3 domain. To determine the effect of the glycosylation sites on IgE Fc synthesis and on Fc epsilon RI alpha binding, site-directed mutagenesis was performed. Mutant IgE Fc constructs were expressed in COS cells and analyzed for protein synthesis and secretion, and Fc epsilon RI alpha binding activity. We find that only N371 and N394 are glycosylated, and that the residues surrounding the glycosylation site at N394 are required for Fc epsilon RI alpha binding activity.
Induction of antigen-specific CD4+ T-cell anergy and deletion by in vivo viral gene transfer (2004)

Immune responses to the therapeutic gene product are a potentially serious complication in treatment of genetic disease by gene therapy. Induction and maintenance of immunologic hypo-responsiveness to the therapeutic antigen is therefore critical to the success of gene-based treatment of inherited protein deficiency. Here, we demonstrate induction of antigen-specific CD4+ T-cell tolerance to a secreted transgene product (ovalbumin, ova) in ova-specific T-cell receptor (TCR) transgenic mice by hepatic adeno-associated virus (AAV)-mediated gene transfer. Transduced mice maintained stable circulating ova levels without evidence of an immune response. Lymph node cells and splenocytes were hypo-responsive to ova as early as day 10 after gene transfer. Numbers of TCR+CD4+ cells were reduced in secondary lymphoid organs and in the thymus by 1 to 2 months after vector administration. The remaining TCR+CD4+ cell population was anergic to ova antigen in vitro and enriched for CD25+ cells. These data provide direct evidence that transgene expression following in vivo viral gene transfer can induce CD4+ T-cell tolerance to the transgene product, involving anergy and deletion mechanisms.
Structural characterization of TaMe3Cl2 and Ta(PMe3)2Me3Cl2, a pair of five and seven-coordinate d0 tantalum methyl compounds (2011)

The trimethylphosphine complex Ta(PMe(3))(2)Me(3)Cl(2) has been synthesized by addition of PMe(3) to TaMe(3)Cl(2). The molecular structures of both TaMe(3)Cl(2) and Ta(PMe(3))(2)Me(3)Cl(2) have been determined by X-ray diffraction thereby demonstrating that, in the solid state, these complexes respectively adopt trigonal bipyramidal and capped trigonal prismatic geometries.
Novel approach to phasing proteins: derivatization by short cryo-soaking with halides (2000)

A quick (less than 1 min) soak of protein crystals in a cryo-solution containing bromide or iodide anions leads to incorporation of these anomalous scatterers into the ordered solvent region around the protein molecules. These halide anions provide a convenient way of phasing through their anomalous scattering signal: bromides using multiwavelength anomalous dispersion (MAD) and bromides and/or iodides using single-wavelength anomalous dispersion (SAD) or single isomorphous replacement with anomalous scattering (SIRAS) methods. This approach has been tested successfully on four different proteins and has been used to solve the structure of a new protein of molecular weight 30 kDa.
Phenotypic Knockout of Nerve Growth Factor in Adult Transgenic Mice Reveals Severe Deficits in Basal Forebrain Cholinergic Neurons, Cell Death in the Spleen, and Skeletal Muscle Dystrophy (2000)

The disruption of the nerve growth factor (NGF) gene in transgenic mice leads to a lethal phenotype (Crowley et al., 1994) and hinders the study of NGF functions in the adult. In this study the phenotypic knockout of NGF in adult mice was achieved by expressing transgenic anti-NGF antibodies, under the control of the human cytomegalovirus promoter. In adult mice, antibody levels are 2000-fold higher than in newborns. Classical NGF targets, including sympathetic and sensory neurons, are severely affected. In the CNS, basal forebrain and hippocampal cholinergic neurons are not affected in the early postnatal period, whereas they are greatly reduced in the adult (55 and 62% reduction, respectively). Adult mice show a reduced ability in spatial learning behavioral tasks. Adult, but not neonatal, transgenic mice further show a new phenotype at the level of peripheral tissues, such as apoptosis in the spleen and dystrophy of skeletal muscles. The analysis of this novel comprehensive transgenic model settles the controversial issue regarding the NGF dependence of cholinergic neurons in adult animals and reveals new NGF functions in adult non-neuronal tissues. The results demonstrate that the decreased availability of NGF in the adult causes phenotypic effects via processes that are at least partially distinct from early developmental effects of NGF deprivation.
Dissolvable Supports in Powder Bed Fusion-Printed Stainless Steel (2017)

This work demonstrates, for the first time, two approaches for dissolvable supports in powder bed fusion-printed metal parts. Expanding on our recent innovations with dissolvable supports in multimaterial direct energy deposition printing, this work brings dissolvable support capabilities to additive manufacturing systems that are limited to single-material builds. First, a direct dissolution approach is demonstrated with the supports electrochemically dissolved. While this process works, it is not self-terminating and maintaining dimensional accuracy for complex geometries is thus difficult. The second approach incorporates a sensitizing agent during the normal stress, relieving thermal annealing step to decrease the chemical stability of the top 100–200 μm of the component surface. The component is then etched under etching conditions with a high selectivity toward the “sensitized” surface over the base component material. This creates an etching process that self-terminates once the sensitized layer is removed. These two processes are first demonstrated using 17-4 PH stainless steel. Direct dissolution was conducted under anodic bias in a solution of HNO3/KCl/HCl; 120 μm of material were removed from the component's surface. For the self-terminating dissolution process, surface sensitization was done by exposing the sample to sodium hexacyanoferrate at 800°C for 6 h to carburize the top 100–200 μm of the sample. This carburization process captures the protective chromium in chromium carbide precipitates and renders the surface sensitive to chemical and electrochemical dissolution. The self-terminating etching reaction was demonstrated under anodic bias in a solution of HNO3/KCl. Open circuit potentials were measured at 10 s and 1 h, and polarization curves were used to identify the corrosion potential. Self-termination was verified by monitoring component diameter over time, and 120 μm of material were removed from the sensitized surface of the component. To further test the self-terminating sensitized approach, a set of 316 stainless steel interlocking rings were fabricated to demonstrate that this approach scales to large components with complex geometry. For these parts, this approach replaced 4–5 days of machining operations with a 32.5 h electrochemical bath.
Preparation and biological properties of a covalent antitumor drug—arm—carrier (DAC conjugate) (1980)

Daunorubicin-HCl was
Performance tradeoffs for client-server query processing (1996)

The construction of high-performance database systems that combine the best aspects of the relational and object-oriented approaches requires the design of client-server architectures that can fully exploit client and server resources in a flexible manner. The two predominant paradigms for client-server query execution are data-shipping and query-shipping We first define these policies in terms of the restrictions they place on operator site selection during query optimization. We then investigate the performance tradeoffs between them for bulk query processing. While each strategy has advantages, neither one on its own is efficient across a wide range of circumstances. We describe and evaluate a more flexible policy called hybrid-shipping, which can execute queries at clients, servers, or any combination of the two. Hybrid-shipping is shown to at least match the best of the two "pure" policies, and in some situations, to perform better than both. The implementation of hybrid-shipping raises a number of difficult problems for query optimization. We describe an initial investigation into the use of a 2-step query optimization strategy as a way of addressing these issues.
The auxin conjugate 1-O-indole-3-acetyl- -D-glucose is synthesized in immature legume seeds by IAGlc synthase and may be used for modification of some high molecular weight compounds (2004)

Immature seeds of some dicotyledonous plants contain IAGlc synthase catalysing the synthesis of 1-O-IAGlc. This enzyme activity is comparable with 1-O-IAGlc synthase activity investigated earlier in liquid endosperm of Zea mays. Polyclonal antibodies against maize 1-O-IAGlc synthase cross-react with partially purified 1-O-IAGlc synthase from immature pea and rape seeds. Single immunoreactive bands were observed at a locus corresponding to 45.7 kDa and 43.7 kDa from pea and rape enzyme preparations, respectively, unlike that from the 50 kDa molecular mass of the maize enzyme. It was also observed that some high molecular weight compounds of pea seeds are labelled in vivo by [(14)C] IAA, and unlabelled 1-O-IAGlc inhibits that labelling. In immature pea seeds 43-49.8% of the IAA-modified high molecular weight compounds, obtained after ultracentrifugation, was found in the soluble fraction and 50.1-57% in the insoluble fraction. Ester-linked IAA accounted for about 6-9% and 38-45.6% in soluble and insoluble material, respectively, estimated after hydrolysis in 1 N NaOH. Enzymatic hydrolysis of IAA-labelled high molecular weight compounds gives free IAA and compound(s) corresponding to IAGlc isomers. These results suggest that 1-O-IAGlc synthesized in legume seeds may be used for the modification of some high molecular weight compounds.
Stick-slip substructure in rapid tape peeling (2010)

The peeling of adhesive tape is known to proceed with a stick-slip mechanism and produces a characteristic ripping sound. The peeling also produces light and when peeled in a vacuum, even X-rays have been observed, whose emissions are correlated with the slip events. Here we present direct imaging of the detachment zone when Scotch tape is peeled off at high speed from a solid surface, revealing a highly regular substructure, during the slip phase. The typical 4-mm-long slip region has a regular substructure of transverse 220 μm wide slip bands, which fracture sideways at speeds over 300 m/s. The fracture tip emits waves into the detached section of the tape at ∼ 100 m/s, which promotes the sound, so characteristic of this phenomenon.