gebhart/patrepeval huggingface dataset:
Differential Regulation of Platelet Aggregation by Matrix Metalloproteinases-9 and -2 (1999)

We have recently found matrix metalloproteinase-2 (MMP-2) in human platelets and reported that the release of this enzyme during platelet activation stimulates aggregation. We have now identified matrix metalloproteinase-9 (MMP-9) in human platelets and resistance-sized (approximately 200 microm) arteries. Resting platelets released small quantities of pro-MMP-9. Maximal release of MMP-9 was detected during partial (appr. 30% maximum) aggregation with thrombin. However, maximal release of MMP-2 was associated with maximal aggregation. MMP-9 antibodies induced aggregation of resting platelets and potentiated aggregation of platelets induced by thrombin and collagen. Moreover, MMP-9 microisolated from arteries as well as recombinant human MMP-9 (0.1-30 ng/ml) inhibited thrombin and collagen-induced aggregation. We conclude that MMP-9 is an inhibitor of aggregation and in this action opposes the effects of MMP-2. The MMP-2/MMP-9 system may play an important role in the regulation of platelet-platelet and platelet-vessel wall interactions.
Polarization investigation of laser-induced breakdown plasma emission from Al, Cu, Mo, W, and Pb elements using nongated detector (2018)

The polarization of plasma emission produced by ablation of Al, Cu, Mo, W, and Pb elements with nanosecond (ns)-laser pulses is explored in the air. A comparison of polarized plasma emission of these metal elements exhibits that the polarization degree increases with increasing the atomic number of elements. It is shown that plasma emission is polarized with no difference between polarization degree of discrete and continuum emission. No improvement in signal to background ratio was found to support the efficiency of polarization resolved laser-induced breakdown method without temporal gating. The estimated collision times of electron-electron and electron-ions ruled out the possibility of highly anisotropic electron distribution function as a case of observed polarization. The polarization due to Fresnel reflection for these elements as a function of wavelengths gives no agreement with the corresponding experimental measurements. In our opinion, polarization emission of laser-induced breakdown plasma may be due to the residual fluctuations from the anisotropic recombining plasma at early stages of the plasma formation and perhaps dynamic polarizability of the core. The enhancing behavior of polarization degree with increasing atomic number reinforces the possibility of the dynamic polarization of a core as a reason of observed polarized radiation in ns-laser-induced breakdown spectroscopy.
[Effect of CPAP and BIPAP on stroke volume in patients with obstructive sleep apnea syndrome]. (1995)

We studied the effect of CPAP and BIPAP on cardiac output (CO) in 23 male obstructive sleep apnea (OSAS) patients with cardiac history but without congestive heart failure (CHF) during wakefulness using the thermodilution method. CPAP was applied at 10 cm H2O and 15 cm H2O, BIPAP at 10/0 cm H2O and 15/10 cm H2O. CO only decreased significantly by 14.6 +/- 11.8% at 15 cm H2O CPAP and by 13.4 +/- 10.0% at 15/10 cm H2O BIPAP. CPAP and BIPAP did not differ in altering hemodynamics. So sleep apnea patients without CHF develop no severe decrease of CO during nasal positive pressure ventilation up to 15 cm 2O.
The functional investigation of a human adenocarcinoma cell line, stably transfected with the neuropeptide Y Y1 receptor (1996)

1. The human adenocarcinoma cell line, HT-29, has been stably transfected with the cDNA sequence for the rat neuropeptide Y (NPY) Y1 receptor, and three Y1 clones (Y1-4, Y1-7 and Y1-16) have been isolated which express high levels of specific [125I]-PYY binding. We have studied the functional responses or lack of responses to peptide YY (PYY) and its analogues in the three transfected clones and HT-29 wild type (wt) cells. 2. Vasoactive intestinal polypeptide (VIP) produced long-lasting increases in short-circuit current (SCC) in both HT-29 wt cells and the Y1 clones. VIP EC50 values were 8.4-11.7 nM in all four cases. The elevation in SCC after a maximal concentration of VIP (30 nM) was significantly greater in Y1-7 cells than in either HT-29 wt epithelia or the other Y1 cell lines. 3. PYY (100 nM) and human pancreatic polypeptide (hPP; 1 microM) were ineffective in HT-29 wt cells under either basal or stimulated conditions. In contrast, basolateral additions of PYY reduced both basal and VIP-stimulated SCC in all three Y1 clones. After VIP, the PYY EC50 values (in nM) were 18.6 in Y1-4, 8.0 in Y1-7 and 52.5 in Y1-16 hPP (1 microM) produced only small and transient responses in each transfected cell type. 4. The Y1 receptor agonist, [Leu31, Pro34] NPY (1 microM) was also effective in the three Y1 cell lines. In the Y1-7 clone the EC50 value for the effect of this peptide was 149 nM, 18.6 fold less potent than PYY. 5. PYY and the Y1-selective non-peptide antagonist, BIBP 3226 displaced [125I]-PYY binding from Y1-7 cell membranes with Ki values of 2.0 and 3.1 nM respectively. In the Y1-7 clone, BIBP 3226 fully inhibited the reductions in VIP-stimulated SCC induced by 30 nM PYY, with an IC50 of 27.2 nM and 30 nM BIBP 3226 caused a parallel rightward shift on the PYY concentration-response curve, with an approximate pKB of 8.0. 6. HT-29 clones stably expressing the Y1 receptor therefore show responses to PYY and its analogues that are characteristic of that subtype, and the Y1-7 clone in particular will be useful in the assessment of novel Y1-specific drugs. This approach will also allow the functional study of NPY Yi receptors with selected mutations.
Semantic Description of Text Mining Services (2012)

Today, a huge amount of crucial business knowl- edge is hidden in unstructured text sources, such as word documents, web pages or forum entries. In order to exploit this knowledge text mining techniques were developed that are able to automatically extract or annotate entities, their relations or sentiments from textual sources. Recently, a number of text mining services that offer REST or SOAP APIs for easy con- sumption were published. These services differ strongly in their mining abilities and result quality and are often constructed for specific use cases. In practice, it is often desirable to combine results of multiple services to increase quality and functionality. However, this result combination is difficult since descriptions of service functionalities are often rarely documented and not standardized so that searching for specific text mining characteristics is time consuming and complex. In this paper we introduce a categorization of text mining services and provide a novel description ontology for describing functional characteristics of a text mining service. The ontology, being of interest for practitioners as well as researchers, is completed by application examples and descriptions that are made publicly available. Through the ontology and the descriptions presented in this paper the automatic use and combination of different text mining services is enabled. Keywords-Text Mining; Semantic Description; Service Ori- ented Architecture.
Cellular barcoding tool for clonal analysis in the hematopoietic system (2010)

Clonal analysis is important for many areas of hematopoietic stem cell research, including in vitro cell expansion, gene therapy, and cancer progression and treatment. A common approach to measure clonality of retrovirally transduced cells is to perform integration site analysis using Southern blotting or polymerase chain reaction-based methods. Although these methods are useful in principle, they generally provide a low-resolution, biased, and incomplete assessment of clonality. To overcome those limitations, we labeled retroviral vectors with random sequence tags or "barcodes." On integration, each vector introduces a unique, identifiable, and heritable mark into the host cell genome, allowing the clonal progeny of each cell to be tracked over time. By coupling the barcoding method to a sequencing-based detection system, we could identify major and minor clones in 2 distinct cell culture systems in vitro and in a long-term transplantation setting. In addition, we demonstrate how clonal analysis can be complemented with transgene expression and integration site analysis. This cellular barcoding tool permits a simple, sensitive assessment of clonality and holds great promise for future gene therapy protocols in humans, and any other applications when clonal tracking is important.
Properties of swollen polymer networks. Solvation and swelling of peptide-containing resins in solid-phase peptide synthesis (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProperties of swollen polymer networks. Solvation and swelling of peptide-containing resins in solid-phase peptide synthesisVirender K. Sarin, Stephen B. H. Kent, and R. B. MerrifieldCite this: J. Am. Chem. Soc. 1980, 102, 17, 5463–5470Publication Date (Print):August 1, 1980Publication History Published online1 May 2002Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/ja00537a006https://doi.org/10.1021/ja00537a006research-articleACS PublicationsRequest reuse permissionsArticle Views1678Altmetric-Citations163LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Intelligent service mediation for supporting advanced location and mobility-aware service provisioning in reconfigurable mobile networks (2002)

Service differentiation, customization, and personalization in the market of 3G mobile services and applications has caused many standards groups, telecommunication companies, and institutions to introduce advanced frameworks and architectures for the support of flexible service provisioning. An important feature in such architectures is location information management, which is a key aspect in future mobile systems and networks, enabling new approaches to service provisioning, customization, and personalization. We present our proposal for a generic open flexible service provisioning architecture that supports location-aware service provisioning and management.
Microcirculation in pancreatic function (1997)

The pancreas is involved in two major bodily functions: production of hormones involved in the control of carbohydrate metabolism and the production of enzymes essential to digestion. Pancreatic function is mediated by both neurological and humoral control. The major pathway for humoral control is through the circulatory system, the level of action being in the microcirculation. This introductory paper explores the need for a deeper understanding of the dynamic morphology, i.e. the actual flow patterns in the microcirculation, as a function of the physiological state and demand to complement the careful ultrastructural mapping of the microvasculature. The current state of knowledge in this field is reviewed as a basis for identifying important areas of knowledge and ignorance, and some suggestions are made as to possible procedures for further experimental studies, particularly in the microscopic observation of the dynamics of the microcirculation with special emphasis on the need for transport studies in both directions across the microvascular wall.
Signed-digit-based multiplier-free realizations for multirate converters (1995)

We propose multiplier-free realizations for FIR multirate converters. Radix-r signed-digit number representation and periodically time-varying (PTV) coefficients are used to obtain multiplier-free realizations. The PTV coefficients, together with one hold-and-scale unit at the input and one scale-add-downsample unit at the output, are utilized effectively to distribute the required computations to the extent that coefficients are highly simplified. The coefficients in the realizations are designed to take values from either the ternary set (0, /spl plusmn/1) or the quinary set (0, /spl plusmn/1, /spl plusmn/2). Their values can be easily obtained by mapping from the target filter coefficients using a radix-r signed-digit number representation. We show that fairly high precision can be achieved by these realizations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Preparation of alpha-emitting 213Bi-labeled antibody constructs for clinical use. (1999)

These data show that 213Bi-labeled antibody constructs can be prepared and administered safely to humans at a wide range of therapeutic levels.
An energy efficient and spectrum efficient wireless heterogeneous network framework for 5G systems (2014)

In this article we explore a system framework of cooperative green heterogeneous networks for 5G wireless communication systems. We first survey the state-of-the-art on spectrum efficiency (SE), energy efficiency (EE), and quality of service (QoS) based mobile association, multi-layer interference management and power control, network wide cooperation and dynamic resource allocation for heterogeneous wireless networks. We also present the system framework of cooperative green heterogeneous networks, which aims at balancing and optimizing SE, EE, and QoS in heterogeneous wireless networks. We discuss the design principles and show some preliminary performance results on the tradeoffs among SE, EE, and QoS. Finally, we identify the technical challenges that remain in the cooperative green heterogeneous network design. The presented wireless system framework is expected to advance the understandings of the critical technical issues toward energy and spectrum efficient 5G wireless communication systems.
Comprehensive high-throughput arrays for relative methylation (CHARM) (2008)

This study was originally conceived to test in a rigorous way the specificity of three major approaches to high-throughput array-based DNA methylation analysis: (1) MeDIP, or methylated DNA immunoprecipitation, an example of antibody-mediated methyl-specific fractionation; (2) HELP, or HpaII tiny fragment enrichment by ligation-mediated PCR, an example of differential amplification of methylated DNA; and (3) fractionation by McrBC, an enzyme that cuts most methylated DNA. These results were validated using 1466 Illumina methylation probes on the GoldenGate methylation assay and further resolved discrepancies among the methods through quantitative methylation pyrosequencing analysis. While all three methods provide useful information, there were significant limitations to each, specifically bias toward CpG islands in MeDIP, relatively incomplete coverage in HELP, and location imprecision in McrBC. However, we found that with an original array design strategy using tiling arrays and statistical procedures that average information from neighboring genomic locations, much improved specificity and sensitivity could be achieved, e.g., approximately 100% sensitivity at 90% specificity with McrBC. We term this approach "comprehensive high-throughput arrays for relative methylation" (CHARM). While this approach was applied to McrBC analysis, the array design and computational algorithms are fractionation method-independent and make this a simple, general, relatively inexpensive tool suitable for genome-wide analysis, and in which individual samples can be assayed reliably at very high density, allowing locus-level genome-wide epigenetic discrimination of individuals, not just groups of samples. Furthermore, unlike the other approaches, CHARM is highly quantitative, a substantial advantage in application to the study of human disease.
Oncostatin M and hepatocyte growth factor induce hepatic maturation via distinct signaling pathways (2001)

Liver development is regulated by soluble factors as well as cell-cell contacts. We previously reported that oncostatin M (OSM) induced hepatic maturation in a primary culture of embryonic day 14 liver cells. While OSM expression in the liver starts in mid gestation and decreases in postnatal stages, hepatocyte growth factor (HGF) is mainly expressed in the liver in the first few days after birth. In this study, we compared the effect of OSM and HGF on the differentiation of fetal hepatic cells in vitro. Like OSM, HGF in the presence of dexamethasone induced expression of glucose-6-phosphatase, tyrosine amino transferase and carbamoyl-phosphate synthase, and accumulation of glycogen in fetal hepatic cells, although to a lesser extent than OSM. Interestingly, while both OSM and HGF up-regulated production of albumin, secretion of albumin occurred only in response to OSM. In addition, although hepatic maturation induced by OSM depends on STAT3, HGF failed to activate STAT3 and HGF-induced differentiation was independent of STAT3. These results indicate that OSM and HGF induce hepatic maturation through different signaling pathways.
Concerted Intercalation and Minor Groove Recognition of DNA by a Homodimeric Thiazole Orange Dye (2000)

The thiazole orange dye TOTO binds to double-stranded DNA (dsDNA) by a sequence selective bis-intercalation. Each chromophore is sandwiched between two base pairs in a (5'-CpT-3'):(5'-ApG-3') site, and the linker spans two base pairs in the minor groove. We have used one- and two-dimensional NMR spectroscopy to examine the dsDNA binding of an analogue of TOTO in which the linker has been modified to contain a bipyridyl group (viologen) that has minor groove binding properties. We have investigated the binding of this analogue, called TOTOBIPY, to three different dsDNA sequences containing a 5'-CTAG-3', a 5'-CTTAG-3', and a 5'-CTATAG-3' sites, respectively, demonstrating that TOTOBIPY prefers to span three base pairs. The many intermolecular NOE connectivities between TOTOBIPY and the d(CGCTTAGCG):d(CGCTAAGCG) oligonucleotide in the complex shows that the bipyridyl-containing linker is positioned in the minor groove and spans three base pairs. Consequently, we have succeeded in designing and synthesizing a ligand that recognizes an extended recognition sequence of dsDNA as the result of a concerted intercalation and minor groove binding mode.
Isoprenoid biosynthesis via the mevalonate-independent route, a novel target for antibacterial drugs? (1998)

Mevalonate was accepted as the universal precursor of all isoprenoids. This assertion was however revealed to be partially incorrect. Incorporation of 13C labeled acetate and glucose into bacterial triterpenoids of the hopane series allowed for the discovery and the partial elucidation of a novel biosynthetic pathway leading to isopentenyl diphosphate. The C5 skeleton of isoprenic units results from the condensation of (hydroxyethyl)thiamin on the carbonyl group of glyceraldehyde 3-phosphate, yielding D-1-deoxyxylulose 1-phosphate. A subsequent intramolecular rearrangement is involved in the formation of the branched isoprenic skeleton. 2C-Methyl-D-erythritol (or its mono- or diphosphate) was shown to be a putative intermediate. This pathway is widespread in bacteria including opportunistic pathogens or innocuous species related to well-known pathogens. In plants, it is involved in the formation of essential chloroplast isoprenoids (carotenoids, phytol, plastoquinone) and of probably most other plastid related isoprenoids of more restricted distribution (isoprene, mono- and diterpenoids). Therefore it potentially represents a novel target for antibacterial drugs and herbicides.
Inactivation of human factor VIII by activated protein C. Cofactor activity of protein S and protective effect of von Willebrand factor. (1988)

Activated protein C (APC) acts as a potent anticoagulant enzyme by inactivating Factor V and Factor VIII. In this study, protein S was shown to increase the inactivation of purified Factor VIII by APC ninefold. The reaction rate was saturated with respect to the concentration of protein S when protein S was present in a 10-fold molar excess over APC. The heavy chain of Factor VIII was cleaved by APC and protein S did not alter the degradation pattern. Factor VIII circulates in a complex with the adhesive protein von Willebrand factor. When purified Factor VIII was recombined with von Willebrand factor, the inactivation of Factor VIII by APC proceeded at a 10-20-fold slower rate as compared with Factor VIII in the absence of von Willebrand factor. Protein S had no effect on the inactivation of the Factor VIII-von Willebrand factor complex by APC. After treatment of this complex with thrombin, however, the actions of APC and protein S towards Factor VIII were completely restored. In hemophilia A plasma, purified Factor VIII associated with endogenous von Willebrand factor, resulting in a complete protection against APC (4 nM). By mixing hemophilic plasma with plasma from a patient with severe von Willebrand's disease, we could vary the amount of von Willebrand factor. 1 U of von Willebrand factor was needed to provide protection of 1 U Factor VIII. Also in plasma from patients with the IIA-type variant of von Willebrand's disease, Factor VIII was protected. In von Willebrand's disease plasma, which was depleted of protein S, APC did not inactivate Factor VIII. These results indicate that protein S serves as a cofactor in the inactivation of Factor VIII and Factor VIIIa by APC and that von Willebrand factor can regulate the action of these two anticoagulant proteins.
The ALK‐5 inhibitor A‐83‐01 inhibits Smad signaling and epithelial‐to‐mesenchymal transition by transforming growth factor‐β (2005)

Transforming growth factor (TGF)-beta signaling facilitates tumor growth and metastasis in advanced cancer. Use of inhibitors of TGF-beta signaling may thus be a novel strategy for the treatment of patients with such cancer. In this study, we synthesized and characterized a small molecule inhibitor, A-83-01, which is structurally similar to previously reported ALK-5 inhibitors developed by Sawyer et al. (2003) and blocks signaling of type I serine/threonine kinase receptors for cytokines of the TGF-beta superfamily (known as activin receptor-like kinases; ALKs). Using a TGF-beta-responsive reporter construct in mammalian cells, we found that A-83-01 inhibited the transcriptional activity induced by TGF-beta type I receptor ALK-5 and that by activin type IB receptor ALK-4 and nodal type I receptor ALK-7, the kinase domains of which are structurally highly related to those of ALK-5. A-83-01 was found to be more potent in the inhibition of ALK5 than a previously described ALK-5 inhibitor, SB-431542, and also to prevent phosphorylation of Smad2/3 and the growth inhibition induced by TGF-beta. In contrast, A-83-01 had little or no effect on bone morphogenetic protein type I receptors, p38 mitogen-activated protein kinase, or extracellular regulated kinase. Consistent with these findings, A-83-01 inhibited the epithelial-to-mesenchymal transition induced by TGF-beta, suggesting that A-83-01 and related molecules may be useful for preventing the progression of advanced cancers.