gebhart/patrepeval huggingface dataset:
Leucocyte elastase as a marker in the diagnosis of acute appendicitis. (1995)

Measurement of the leucocyte elastase complex in plasma does not increase the accuracy of the diagnosis of acute appendicitis. There was no significant correlation between the leucocyte elastase activity and the total white cell count.
Store depletion and store‐operated Ca2+ current in human prostate cancer LNCaP cells: involvement in apoptosis (2000)

1. In the present study, we investigated the mechanisms involved in the induction of apoptosis by the Ca2+-ATPase inhibitor thapsigargin (TG), in androgen-sensitive human prostate cancer LNCaP cells. 2. Exposure of fura-2-loaded LNCaP cells to TG in the presence of extracellular calcium produced an increase in intracellular Ca2+, the first phase of which was associated with depletion of intracellular stores and the second one with consecutive extracellular Ca2+ entry through plasma membrane, store-operated Ca2+ channels (SOCs). 3. For the first time we have identified and characterized the SOC-mediated membrane current (Istore) in prostate cells using whole-cell, cell-attached, and perforated patch-clamp techniques, combined with fura-2 microspectrofluorimetric and Ca2+-imaging measurements. 4. Istore in LNCaP cells lacked voltage-dependent gating and displayed an inwardly rectifying current-voltage relationship. The unitary conductance of SOCs with 80 mM Ca2+ as a charge carrier was estimated at 3.2 +/- 0.4 pS. The channel has a high selectivity for Ca2+ over monovalent cations and is inhibited by Ni2+ (0.5-3 mM) and La3+ (1 microM). 5. Treatment of LNCaP cells with TG (0.1 microM) induced apoptosis as judged from morphological changes. Decreasing extracellular free Ca2+ to 200 nM or adding 0.5 mM Ni2+ enhanced TG-induced apoptosis. 6. The ability of TG to induce apoptosis was not reduced by loading the cells with intracellular Ca2+ chelator (BAPTA-AM). 7. These results indicate that in androgen-sensitive prostate cancer cells the depletion of intracellular Ca2+ stores may trigger apoptosis but that there is no requirement for the activation of store-activated Ca2+ current and sustained Ca2+ entry in induction and development of programmed cell death.
Aspiration to Obtain Osteoblast Progenitor Cells from Human Bone Marrow (1997)

On the basis of these data, we recommend that, when bone marrow is obtained with aspiration for use as a bone graft, the volume of aspiration from any one site should not be greater than two milliliters. A larger volume decreases the concentration of osteoblast progenitor cells because of dilution of the bone-marrow sample with peripheral blood. We estimate that four one-milliliter aspirates will provide almost twice the number of alkaline phosphatase-positive colony-forming units as will one four-milliliter aspirate. In addition, these data confirm that humans differ significantly from one another with respect to the cellularity of bone marrow and the prevalence of osteoblast progenitor cells. Additional studies are necessary to determine if the number or prevalence of alkaline phosphatase-positive colony-forming units in bone marrow is a determining factor in the efficacy of an autogenous bone or bone-marrow graft and to ascertain how the number and function of alkaline phosphatase-positive colony-forming units may change as a function of factors such as age, menopausal status, and selected diseases.
New Trick for an Old Ligand! The Sensing of Zn(II) Using a Lanthanide Based Ternary Yb(III)-cyclen-8-hydroxyquinoline System As a Dual Emissive Probe for Displacement Assay (2012)

A novel near-infrared (NIR) emissive lanthanide-based zinc sensor was designed, based on the self-assembly in aqueous solution between the nonemissive coordinatively unsaturated Yb(III) cyclen complex 2·Yb and the sulfonated 8-hydroxyquinoline (8-HQS) chromophore, which was employed as a sensitizing antenna. The resulting ternary complex, 2·Yb·8-HQS, displayed metal-centered emission in the NIR range upon excitation of the antenna with high quantum yield of Q = 0.23 ± 0.03% in pH 7.4 buffered aqueous solution; demonstrating efficient sensitization from 8-HQS. The addition of zinc led to quenching of the NIR emission as a result of the dissociation of the luminescent ternary 2·Yb·8-HQS complex, where the 8-HQS antenna was displaced from the Yb(III) center in favor of the formation of more stable chelates with Zn(II). These newly formed Zn(II) complexes were shown to exhibit strong green fluorescence; allowing for the simultaneous sensing of Zn(II) both within the visible and the NIR regions at physiological pH in competitive media. Furthermore, 2·Yb·8-HQS was shown to be able to detect Zn(II) with good selectivity and in a reversible manner, even in the presence of competitive group (I) and (II) metal ions as well as in the presence of several biologically important d-metal ions.
Simulation methods and tissue property models for non-invasive transcranial focused ultrasound surgery (2005)

Thesis (Ph. D.)--Harvard-MIT Division of Health Sciences and Technology, 2005.
Expression of Blood-Group Antigen A — A Favorable Prognostic Factor in Non-Small-Cell Lung Cancer (1991)

Expression of blood-group antigen A in tumor cells is an important favorable prognostic factor in patients with non-small-cell lung cancer. This variable needs to be considered in the design of future trials of therapy.
Specific recognition of antibody-oligonucleotide conjugates by radiolabeled antisense nucleotides: a novel approach for two-step radioimmunotherapy of cancer (1993)

One of the major challenges in radioimmunotherapy is the specific delivery of radioisotopes to tumor cells while minimizing normal tissue radiation. In this respect, the application of two-step pretargeting schemes generally leads to more favorable tumor to normal tissue uptake ratios than direct administration of radioimmunoconjugates. In this study, we present the specific hybridization of complementary DNA fragments as a novel recognition mechanism in pretargeting. Briefly, our strategy involves first administration of antibody-DNA conjugate, followed by targeting with radiolabeled complementary DNA (antisense DNA). Complementary oligodeoxynucleotides (14-mers, Tm = 57 degrees C), in which part of the phosphodiesters has been replaced by methylphosphonates (to ensure stability against nucleases), were prepared on a DNA synthesizer. The oligonucleotides were further derivatized via a uridine moiety at their 5'-end in such a way that radiolabeling or conjugation with antibodies could be accomplished. Both a murine IgG (anti-hCG) and the human anti-tumor IgM 16.88 were conjugated with one to three oligonucleotides via the heterobifunctional cross-linker SMCC. Incubation of these immunoconjugates with the radiolabeled antisense DNA revealed specific hybridization with the antibody-linked oligonucleotides. Antigen binding studies performed with antigen-coated matrices showed that the immunoreactivity of the antibody-DNA conjugate is preserved. Moreover, it is demonstrated that the radiolabeled DNA is still capable of hybridizing selectively with the oligonucleotides of the immunoconjugate, when the latter is bound to its antigen.
Causes of death in racehorses over a 2 year period (1994)

Necropsies were performed on 496 horses that had a fatal injury or illness at a California racetrack during the period February 20th 1990 to March 1st 1992. The primary cause of death was categorised by breed, activity at time of injury or illness and organ system affected. Most of the submissions were Thoroughbred horses (432) and Quarter Horses (46). Most of the injuries occurred while racing (42%) and in training sessions (39%); with fewer non-exercise (12%) and accident (7%) related injuries or illnesses. Musculoskeletal injuries accounted for 83% of the Thoroughbred and 80% of the Quarter Horse submissions. The Thoroughbred horses incurred 306 fractures with 263 in the limbs and 90% of those in the forelimbs. The proximal sesamoid bone(s), third metacarpal bone and humerus were the most common bones fractured in Thoroughbred horses and Quarter Horses. Other major causes of death included respiratory, digestive and multi-organ system disorders.
Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c. (1987)

The CD1 human antigens are a family of at least three components, CD1a, CD1b, and CD1c, that are characteristic of the cortical stage of thymocyte maturation. CD1a was originally named HTA1 or T6 and thought to be the human equivalent of mouse Tla. The genes coding for all three have now been identified by transfection into mouse cells. The transfectants express the surface antigens that can then be recognized by the corresponding cluster of monoclonal antibodies used to define the three members of CD1. The full sequence of the genomic DNA is described for all three. The intron-exon structure of CD1a is deduced by comparison with a near-full-length cDNA clone. Similar structures are proposed for the other two, largely based on sequence homology. An unusually long 5'-untranslated exon (280 bases long) is highly conserved between the three genes, suggesting an important but unknown function. CD1c has a duplicated form of this exon that is thought to be spliced out. The major homology between the three antigens is in the beta 2-microglobulin-binding domain. The general relatedness to major histocompatibility complex class I and class II molecules is significant but low, with no section of higher homology to mouse Tla.
Human Lactoferrin and Peptides Derived from Its N Terminus Are Highly Effective against Infections with Antibiotic-Resistant Bacteria (2001)

Since human lactoferrin (hLF) binds to bacterial products through its highly positively charged N terminus, we investigated which of the two cationic domains is involved in its bactericidal activity. The results revealed that hLF lacking the first three residues (hLF(-3N)) was less efficient than hLF in killing of antibiotic-resistant Staphylococcus aureus, Listeria monocytogenes, and Klebsiella pneumoniae. Both hLF preparations failed to kill Escherichia coli O54. In addition, hLF(-3N) was less effective than hLF in reducing the number of viable bacteria in mice infected with antibiotic-resistant S. aureus and K. pneumoniae. Studies with synthetic peptides corresponding to the first 11 N-terminal amino acids, designated hLF(1-11), and fragments thereof demonstrated that peptides lacking the first three N-terminal residues are less effective than hLF(1-11) in killing of bacteria. Furthermore, a peptide corresponding to residues 21 to 31, which comprises the second cationic domain, was less effective than hLF(1-11) in killing of bacteria in vitro and in mice having an infection with antibiotic-resistant S. aureus or K. pneumoniae. Using fluorescent probes, we found that bactericidal hLF peptides, but not nonbactericidal peptides, caused an increase of the membrane permeability. In addition, hLF killed the various bacteria, most probably by inducing intracellular changes in these bacteria without affecting the membrane permeability. Together, hLF and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant S. aureus and K. pneumoniae, and the first two arginines play an essential role in this activity.
Ribitol dehydrogenase of Klebsiella aerogenes. Sequence of the structural gene (1985)

The ribitol dehydrogenase gene was cloned from wild-type Klebsiella aerogenes and also from a transducing phage lambda prbt which expresses the rbt operon constitutively. The coding sequence for 249 amino acids is separated from the following D-ribulokinase gene by 31 base pairs containing three stop codons, one of which overlaps the ribosome binding site for D-ribulokinase. Three residues in the amino acid sequence differ from that predicted from the DNA sequence: Asp-212 for Asn-212 is probably a protein sequencing error, but -Ala-Val- for -Ser-Ser- at 146-147 appears to be a 'neutral mutation' that may have arisen during prolonged chemostat selection of a strain that superproduces the enzyme from which the protein sequence was determined.
The transactional HW/SW stack for fault tolerant embedded computing (2015)

O desafio de implementar tolerância a falhas em sistemas embarcados advém das restrições físicas de ocupação de área, dissipação de potência e consumo de energia desses sistemas. A necessidade de otimizar essas três restrições de projeto concomitante à computação dentro dos requisitos de desempenho e de tempo-real cria um problema difícil de ser resolvido. Soluções clássicas de tolerância a falhas tais como redundância modular dupla e tripla não são factíveis devido ao alto custo em potência e a falta de um mecanismo para se recuperar erros. Apesar de algumas técnicas existentes reduzirem o overhead de potência e área, essas incorrem em alta degradação de desempenho e muitas vezes assumem um modelo de falhas que não é factível. Essa tese introduz a Pilha de HW/SW Transacional, ou simplesmente Pilha, para gerenciar de maneira eficiente as restrições de área, potência, cobertura de falhas e desempenho. A Pilha introduz uma nova estratégia de compilação que organiza os programas em Blocos Básicos Transacionais (BBT), juntamente com um novo processador, a Arquitetura de Blocos Básicos Transacionais (ABBT), a qual provê detecção e recuperação de erros de grão fino e determinística ao usar o BBT como um contâiner de erros e como unidade de checkpointing. Duas soluções para prover a semântica de execução do BBT em hardware são propostas, uma baseada em software e a outra em hardware. A área, potência, desempenho e cobertura de falhas foram avaliadas através do modelo de hardware do ABBT. A Pilha provê uma cobertura de falhas de 99,35%, com overhead de 2,05 em potência e 2,65 de área. A Pilha apresenta overhead de desempenho de 1,33 e 1,54, dependento do modelo de hardware usado para suportar a semântica de execução do BBT.
SPARC‐like 1 (SC1) is a diversely expressed and developmentally regulated matricellular protein that does not compensate for the absence of SPARC in the CNS (2011)

SPARC-like 1 (SC1) is a member of the SPARC family of matricellular proteins that has been implicated in the regulation of processes such as cell migration, proliferation, and differentiation. Here we show that SC1 exhibits remarkably diverse and dynamic expression in the developing and adult nervous system. During development, SC1 localizes to radial glia and pial-derived structures, including the vasculature, choroid plexus, and pial membranes. SC1 is not downregulated in postnatal development, but its expression shifts to distinct time windows in subtypes of glia and neurons, including astrocytes, large projection neurons, Bergmann glia, Schwann cells, and ganglionic satellite cells. In addition, SC1 expression levels and patterns are not altered in the SPARC null mouse, suggesting that SC1 does not compensate for the absence of SPARC. We conclude that SC1 and SPARC may share significant homology, but are likely to have distinct but complementary roles in nervous system development.
Binding of Bone Sialoprotein, Osteopontin and Synthetic Polypeptides to Hydroxyapatite (2001)

The phosphorylated acidic glycoproteins bone sialoprotein (BSP) and osteopontin (OPN) bind to hydroxyapatite (HA) crystals and may be involved in the regulation of bone mineralization. The HA-binding properties of these proteins have been attributed to glutamic acid-rich sequences in BSP and aspartic acid-rich sequences in OPN. The present study examines the roles of these polycarboxylate sequences in the binding of BSP and OPN to HA. Porcine BSP, OPN and the synthetic polypeptides poly-L-glutamic acid [Poly(Glu)] and poly-L-aspartic acid [Poly(Asp)] were labeled with fluorescein isothiocyanate and their binding to HA determined by fluorimetry. From the binding isotherms, dissociation constants (KDs) for all the reagents tested were determined to be in the micromolar range. The saturation binding capacities of HA for Poly(Glu), Poly(Asp), BSP and OPN were similar (500-600 micrograms/m2). To investigate the role of glutamic acid-rich and aspartic acid-rich sequences in the binding to HA of BSP and OPN, respectively, competitive binding studies with Poly(Glu) and Poly(Asp) were performed. Poly(Glu) was able to displace a maximum of 100% of Poly(Glu), 81% of OPN, 68% of BSP and 65% of Poly(Asp). Poly(Asp) was able to displace a maximum of 100% of Poly(Glu), 99% of Poly(Asp), 95% of OPN and 89% of BSP. These results are consistent with the view that BSP and OPN bind to HA via their polycarboxylate sequences, but suggest a complex mode of interaction between polyelectrolytes and ionic crystals.
Parallelisation of Digital Signal Processing in Uniform and Reconfigurable Filter Banks for Satellite Communications (2006)

Modern satellite communication calls for novel and flexible concepts for de- and remultiplexing of wide-band FDM-signals in conjunction with beam switching on-board a satellite. For this application, two highly efficient approaches to uniform and yet reconfigurable digital filter banks are known: i) the complex-modulated polyphase filter bank, and ii) the tree-structured filter bank. Channelising and remultiplexing of (ultra-)wide-band FDM-signals require high end sample rates that, often, range beyond technological limit (e.g. > l GHz). A remedy is to process the signals at a lower rate in a parallel manner. While the polyphase approach i) applying one-step sample rate alteration (down/up-sampling) inherently operates in parallel, the hierarchical approach ii) being based on stepwise sample rate alteration calls for the parallelisation of, at least, the high rate stages. In this contribution, the systematic sample-by-sample processing procedure for parallelisation of multirate systems by Groth is recalled. It is applied to the parallelisation of a novel class of tree-structured filter banks: The various steps towards parallelisation of the high rate front end of the FDM-demultiplexer part of the filter bank are described in detail
Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3) (2012)

In the present study, T. fusca cutinase was successfully secreted to the culture media by α-hemolysin secretion system. This is the first report of cutinase being efficiently secreted by this pathway. Due to the limited cases of successful expression of industrial enzyme by E. coli α-hemolysin secretion system, our study further explored the utilization of this pathway in industrial enzymes.
Affinity modulation of small-molecule ligands by borrowing endogenous protein surfaces (1999)

A general strategy is described for improving the binding properties of small-molecule ligands to protein targets. A bifunctional molecule is created by chemically linking a ligand of interest to another small molecule that binds tightly to a second protein. When the ligand of interest is presented to the target protein by the second protein, additional protein-protein interactions outside of the ligand-binding sites serve either to increase or decrease the affinity of the binding event. We have applied this approach to an intractable target, the SH2 domain, and demonstrate a 3-fold enhancement over the natural peptide. This approach provides a way to modulate the potency and specificity of biologically active compounds.
Inheritance of Resistance to the Soybean Aphid in Soybean PI 200538 (2009)

The soybean aphid ( Aphis glycines Matsumura) is a major soybean [ Glycine max (L.) Merr.] insect pest. Soybean plant introduction (PI) 200538 has strong resistance to the aphid. The objectives of our research were to determine the inheritance of resistance and to map gene(s) controlling resistance in PI 200538. F 2 populations developed from crosses between PI 200538 and three susceptible genotypes were tested for resistance and with DNA markers. F 2 plants from the cross ‘Ina’ × PI 200538 segregated 114 resistant to 37 susceptible and F 2 plants from the cross ‘Williams 82’ × PI 200538 segregated 203 resistant to 65 susceptible when tested for resistance to soybean aphid biotype 1. F 2 plants from the cross LD02‐4485 × PI 200538 segregated 167 resistant to 62 susceptible when tested for resistance to biotype 2. These populations fit a 3:1 genetic ratio ( P = 0.89, 0.78, and 0.52, respectively) with resistance dominant over susceptibility. Segregation among F 2:3 families from the crosses supported the dominant resistance gene hypothesis. The gene mapped to soybean linkage group F, flanked by the simple sequence repeat marker loci Satt510, Soyhsp176, Satt114, and Sct_033, located in the same region as the aphid resistance gene Rag2 Since the resistance gene in PI 200538 also gave resistance to soybean aphid biotypes 1 and 2, it is possible that the gene is Rag2 and not a new aphid resistance gene. Therefore, PI 200538 may be an additional source of Rag2
Peptide toxins from Conus geographus venom. (1981)

Three homologous toxic peptides which cause postsynaptic inhibition at the vertebrate neuromuscular junction have been purified from the venom of the marine snail Conus geographus. Their amino acid sequences are: (formula see text) The biologically active peptides are monomeric, with internal disulfide bonds.
In Situ Regulation of DC Subsets and T Cells Mediates Tumor Regression in Mice (2009)

Vaccines are largely ineffective for patients with established cancer, as advanced disease requires potent and sustained activation of CD8(+) cytotoxic T lymphocytes (CTLs) to kill tumor cells and clear the disease. Recent studies have found that subsets of dendritic cells (DCs) specialize in antigen cross-presentation and in the production of cytokines, which regulate both CTLs and T regulatory (Treg) cells that shut down effector T cell responses. Here, we addressed the hypothesis that coordinated regulation of a DC network, and plasmacytoid DCs (pDCs) and CD8(+) DCs in particular, could enhance host immunity in mice. We used functionalized biomaterials incorporating various combinations of an inflammatory cytokine, immune danger signal, and tumor lysates to control the activation and localization of host DC populations in situ. The numbers of pDCs and CD8(+) DCs, and the endogenous production of interleukin-12, all correlated strongly with the magnitude of protective antitumor immunity and the generation of potent CD8(+) CTLs. Vaccination by this method maintained local and systemic CTL responses for extended periods while inhibiting FoxP3 Treg activity during antigen clearance, resulting in complete regression of distant and established melanoma tumors. The efficacy of this vaccine as a monotherapy against large invasive tumors may be a result of the local activity of pDCs and CD8(+) DCs induced by persistent danger and antigen signaling at the vaccine site. These results indicate that a critical pattern of DC subsets correlates with the evolution of therapeutic antitumor responses and provide a template for future vaccine design.
Conversion Limits in the Reaction of CO2with Lime (2003)

The use of calcines of natural limestones as CO2 regenerable sorbents is investigated in this work by studying the decay of the maximum carbonation conversion during many carbonation/calcination cycles. New experimental information is complemented with a compilation of previously published data on this subject. The observed conversion limits in the reaction of CO2 with lime are interpreted in terms of a certain loss in the porosity associated with small pores and a certain increase in the porosity associated with large pores. In the carbonation part of every cycle, the CaCO3 fills up all the available porosity made up of small pores plus a small fraction of the large voids, limited by the thickness of the product layer that marks the onset of the slow carbonation rate. A simple model based on textural changes, observed by scanning electron microscopy, fits equally well all the data from this work and from other authors. The two model parameters are consistent with known mechanism occurring during calcination and carbonation.