gebhart/patrepeval huggingface dataset:
Overexpression of the c-met Protooncogene in Human Gastric Carcinoma - Correlation to Clinical Features (2001)

Overexpression of hepatocyte growth factor receptor (c-met) has been detected in many human tumors. To investigate the possible involvement of c-met in human gastric carcinogenesis, we examined c-met expression in 45 patients with gastric carcinoma using Northern blot analysis, reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemical staining. The c-met mRNA expression was increased twofold and sevenfold in gastric carcinoma tissues compared to the adjacent normal tissues by Northern blot analysis and RT-PCR, respectively. In the immunohistochemical study, c-met protein was detected in 32 of 45 (71.1%) patients, with marked overexpression in gastric carcinoma compared with matched normal gastric tissues. The c-met-positive immunoreactivities were more frequently encountered in serosa-exposed and serosa-infiltrating gastric cancer (p = 0.003). In addition, tumor stage was another statistically significant parameter that was observed between the two groups (p = 0.02). Multivariate analyses revealed that the tumor stage (p = 0.014) and c-met overexpression (p = 0.031) were independently correlated with survival. These data suggest that overexpression of c-met may play a part in gastric carcinogenesis and may represent a prognostic factor for gastric cancer.
TheChlamydomonasFus1 Protein Is Present on the Mating TypeplusFusion Organelle and Required for a Critical Membrane Adhesion Event during Fusion withminusGametes (2003)

The molecular mechanisms of the defining event in fertilization, gamete fusion, remain poorly understood. The FUS1 gene in the unicellular, biflagellated green alga Chlamydomonas is one of the few sex-specific eukaryotic genes shown by genetic analysis to be essential for gamete fusion during fertilization. In Chlamydomonas, adhesion and fusion of the plasma membranes of activated mt+ and mt- gametes is accomplished via specialized fusion organelles called mating structures. Herein, we identify the endogenous Fus1 protein, test the idea that Fus1 is at the site of fusion, and identify the step in fusion that requires Fus1. Our results show that Fus1 is a approximately 95-kDa protein present on the external surface of both unactivated and activated mt+ gametes. Bioassays indicate that adhesion between mating type plus and mating type minus fusion organelles requires Fus1 and that Fus1 is functional only after gamete activation. Finally, immunofluorescence demonstrates that the Fus1 protein is present as an apical patch on unactivated gametes and redistributes during gamete activation over the entire surface of the microvillous-like activated plus mating structure, the fertilization tubule. Thus, Fus1 is present on mt+ gametes at the site of cell-cell fusion and essential for an early step in the fusion process.
Oxide electrodes as barriers to hydrogen damage of Pb(Zr,Ti)O3-based ferroelectric capacitors (1999)

The ferroelectric properties of Pb(Zr,Ti)O3 films are known to degrade when subjected to forming gas anneals. In an earlier publication we established that although there may be loss of oxygen and lead during forming gas anneal, the primary mechanism for loss of ferroelectricity is the incorporation of hydrogen and subsequent formation of [OH]−1 bonds between the ionized hydrogen and oxygen ions along the polarization axis in the octahedra. In this study, we show that (La,Sr)CoO3 oxide electrodes can act as a diffusion barrier to hydrogen during forming gas anneals. Forming gas anneal at lower temperatures such as 200 and 300 °C does not lead to a measurable loss of polarization. There is some loss of polarization during forming gas anneals at 450 °C for 12 h, however the capacitors still exhibit ferroelectric properties. The capacitors show no fatigue up to 1011 cycles, no imprint, good logic state retention characteristics, and similar slopes for the pulse width dependent polarization values before and after forming gas anneal in the measured range. More importantly, we have demonstrated that even a bare La0.5Sr0.5CoO3 top electrode can prevent the complete loss of ferroelectricity during forming gas treatment.
Microwave Facilitation of Methylnaltrexone Antagonism of Morphine-Induced Analgesia in Mice (1986)

Exposure to low dose microwave irradiation has been postulated to influence effects of centrally active pharmacological agents. In this investigation, the analgesic dose, 50% (ADSO) for morphine in the mouse tail flick test was significantly increased by pretreatment with the centrally active opiate receptor blocker naltrexone, and the dose-response curve ms shifted significantly to the right. On the other hand, the AD50 and dose-response curve for morphine were not influenced by either the peripherally active opiate receptor blocker methylnaltrexone alone or microwave irradiation (2.45 GHz, 20 mW/cm2, CW, 10 min) alone. However, following both methylnaltrexone pretreatment and microwave exposure, there was both a significant increase in the AD50 as well as a significant rightward shift of the dose-response curve, indicating a competitive antagonism of morphine by methylnaltrexone. One possible explanation for this microwave-drug interaction is that microwave energy might facilitate entry of methylnaltrexone into the central nervous system.
A quiet revolution: opportunities for local futures in the UK (2002)

Central Government in the UK has opened the door to futures work at a local level through the modernisation programme for local government. It has created conditions for local governance which are strategic and futures‐orientated. This raises challenges and opportunities for developing expert and participative futures research to inform local thinking, decision making and action. It also opens the institutions, structures and processes at a local level to the inherently uncomfortable and destabilising thoughts of what the future may hold within an interconnected and turbulent world. This work appears to be a quiet revolution in the making, creating major opportunities for foresight, futures research and anticipatory management at a local level and for developing processes that focus civic attention on a regular basis on long‐term challenges and implications for personal, corporate and political action.
Involvement of NK1+ T cells and their IFN-gamma production in the generalized Shwartzman reaction. (1998)

IL-12 (or LPS) priming and subsequent challenge by LPS produces the generalized Shwartzman reaction. IFN-gamma induced by IL-12 is a crucial cytokine in the priming phase. In vivo depletion of both NK cells and NK1+ alphabeta T cells of mice by anti-NK1.1 Ab greatly reduced the elevation of serum IFN-gamma induced by IL-12 and significantly reduced mortality after subsequent injection of LPS, whereas depletion of NK cells alone by anti-asialo GM1 Ab only partially decreased serum IFN-gamma, and lethality was not changed. Cell sorting and culture experiments confirmed that liver NK1+ alphabeta T cells of IL-12-injected mice produced greater amounts of IFN-gamma than did liver NK cells. MHC class I-deficient mice of C57BL/6 background, which lack a majority of NK1+ alphabeta T cells, produced low amounts of IFN-gamma by IL-12; no mortality was observed after the LPS challenge. However, production of TNF-alpha in the second phase (after LPS challenge) was not inhibited by depletion of NK cells alone or both subsets. IL-12 and subsequent LPS challenge activated NK1+ alphabeta T cells in the liver and induced strong cytotoxicity of these cells not only against tumor cells (including Fas-negative tumors) but also against a syngeneic hepatocyte cell line. Our findings show that IFN-gamma produced by NK1+ alphabeta T cells is essential for the IL-12 priming of the Shwartzman reaction, and the autoreactivity of NK1+ alphabeta T cells in the liver is involved in the hepatic disorders that are sometimes caused by IL-12, LPS, or the generalized Shwartzman reaction.
Production and characterization of transgenic pigs expressing porcine CTLA4‐Ig (2009)

Viable pigs exhibiting robust and ubiquitous expression of pCTLA4-Ig were produced on both a WT and GTKO background. Expression of pCTLA4-Ig resulted in acute susceptibility to opportunistic pathogens due at least in part to a significantly compromised humoral immune status. As this molecule is known to have immunosuppressive activity, high levels of pCTLA4-Ig expression in the blood, as well as defective development related to exposure to pCTLA4-Ig in utero, may contribute to this reduced immune status. Prophylactic treatment with antibiotics may promote survival of disease-free transgenic pigs to a size optimal for organ procurement for transplantation. Additional genetic modifications and/or tightly regulated expression of pCTLA4Ig may reduce the impact of this transgene on the humoral immune system.
Insertional inactivation studies of the csmA and csmC genes of the green sulfur bacterium Chlorobium vibrioforme 8327: the chlorosome protein CsmA is required for viability but CsmC is dispensable (1998)

Targeted mutagenesis was used to investigate the roles of the CsmA and CsmC proteins of the chlorosomes of the green bacteria Chlorobium tepidum and Chlorobium vibrioforme 8327. Under the photoautotrophic growth conditions employed, CsmA is required for the viability of the cells but CsmC is dispensable. The absence of CsmC caused a small red shift in the near-infrared absorption maximum of bacteriochlorophyll d in whole cells and chlorosomes, but chlorosomes were assembled in and could be isolated from the csmC mutant. The doubling time of the csmC mutant was approximately twice that of the wild-type strain. Fluorescence emission measurements suggested that energy transfer from the bulk bacteriochlorophyll d to another pigment, perhaps bacteriochlorophyll a, emitting at 800–804 nm, was less efficient in the csmC mutant cells than in wild-type cells. These studies establish that transformation and homologous recombination can be employed in targeted mutagenesis of Chlorobium sp. and further demonstrate that chlorosome proteins play important roles in the structure and function of these light-harvesting organelles.
Home 3D body scans from noisy image and range data (2011)

The 3D shape of the human body is useful for applications in fitness, games and apparel. Accurate body scanners, however, are expensive, limiting the availability of 3D body models. We present a method for human shape reconstruction from noisy monocular image and range data using a single inexpensive commodity sensor. The approach combines low-resolution image silhouettes with coarse range data to estimate a parametric model of the body. Accurate 3D shape estimates are obtained by combining multiple monocular views of a person moving in front of the sensor. To cope with varying body pose, we use a SCAPE body model which factors 3D body shape and pose variations. This enables the estimation of a single consistent shape while allowing pose to vary. Additionally, we describe a novel method to minimize the distance between the projected 3D body contour and the image silhouette that uses analytic derivatives of the objective function. We propose a simple method to estimate standard body measurements from the recovered SCAPE model and show that the accuracy of our method is competitive with commercial body scanning systems costing orders of magnitude more.
Molecular Cloning, Refined Physical Map and Heterogeneity of Methylation Sites of Papilloma Virus Type 1a DNA (1980)

The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. The integrity and the homogeneity of the viral DNA thus obtained was confirmed by restriction endonucleases analysis. Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome.
An in Vitro System for Studying the Kinetics of Interchain Disulfide Bond Formation in Immunoglobulin G (1974)

Abstract An in vitro system for studying the order and kinetics of interchain disulfide bond formation (covalent assembly) in immunoglobulins has been developed using subclasses 1 and 4 of human immunoglobulin G. Noncovalent association of reduced, but not alkylated, heavy and light chains was achieved at pH 4.8 and air oxidation initiated by rapid transfer to aerated buffer at pH 8.2. Disulfide-bonded species in samples taken at various times were detected, following alkylation, by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The principal intermediates in the covalent assembly of immunoglobulin G1, with either kappa or lambda light chains, were half-molecules (HL) and H2L. The half-time for the appearance of the completed molecule, H2L2, ranged from 70 to 95 min at 25°. Half-molecules were the predominant intermediate in the assembly of immunoglobulin G4. The disulfide bonds between the heavy chains formed slowly; H2L2 appeared with a half-time of 16 hours. The effects of temperature, pH, cupric ion concentration, and reducing agent on the air oxidation of reduced immunoglobulin were evaluated. Although all these parameters influenced the rate of H2L2 production, the order of disulfide bond formation was unaffected except in the presence of high concentrations of cupric ion. Covalent assembly utilizing a thiol-disulfide interchange system (oxidized and reduced glutathione) was also examined. Optimum rates of H2L2 production were observed with 10-2 m glutathione + 10-3 m oxidized glutathione. For immunoglobulin G1 assembly the half-time was reduced to 25 min at 25° and 10 min at 37° and to 30 min at 37° for subclass 4. These rates are comparable to those observed for covalent assembly in vivo.
Targeting of drugs 6 : strategies for stealth therapeutic systems (1998)

Interactions between Blood Components and Artificial Surfaces K.D. Caldwell. The Mononuclear Phagocyte System: Features Relevant to Interactions with Liposomes S. Gordon. StealthTM Therapeutic Systems: Rationale and Strategies D.J.A. Crommelin, G. Storm. Long Circulating Liposomes: Evolution of the Concept G. Gregoriadis. Sterically Stabilized Immunoliposomes: Formulations for Delivery of Drugs and Genes to Tumor Cells in Vivo J.W. Park, et al. Pegylation of Liposomes in Cell-Specific Targeting: It Does Not Always Make Sense G.L. Scherphof, et al. StealthTM Liposomes for the Targeting of Drugs in Cancer Therapy T.M. Allen, et al. StealthTM Liposomes as Carriers of Doxorubicin D. Goren, et al. Liposome-Mediated Delivery of Retinoids K. Mehta, et al. Applications of Liposome Technology to Overcome Multidrug Resistance in Solid Tumors R. Krishna, L.D. Mayer. Use of Radiolabeled Liposomes for PEG-Liposome-Based Drug Targeting and Diagnostic Imaging Applications W.T. Phillips. Diagnostic and Therapeutic Targeting of Infectious and Inflammatory Diseases Using Sterically Stabilized Liposomes G. Storm, et al. Biologically Active Ligand-Bearing Polymer-Grafted Liposomes S. Zalipsky, et al. 11 Additional Articles. Index.
The Garp architecture and C compiler (2000)

Various projects and products have been built using off-the-shelf field-programmable gate arrays (FPGAs) as computation accelerators for specific tasks. Such systems typically connect one or more FPGAs to the host computer via an I/O bus. Some have shown remarkable speedups, albeit limited to specific application domains. Many factors limit the general usefulness of such systems. Long reconfiguration times prevent the acceleration of applications that spread their time over many different tasks. Low-bandwidth paths for data transfer limit the usefulness of such systems to tasks that have a high computation-to-memory-bandwidth ratio. In addition, standard FPGA tools require hardware design expertise which is beyond the knowledge of most programmers. To help investigate the viability of connected FPGA systems, the authors designed their own architecture called Garp and experimented with running applications on it. They are also investigating whether Garp's design enables automatic, fast, effective compilation across a broad range of applications. They present their results in this article.
Regulation of Microtubule-Dependent Protein Transport by the TSC2/Mammalian Target of Rapamycin Pathway (2006)

Protein transport plays a critical role in the interaction of the cell with its environment. Recent studies have identified TSC1 and TSC2, two tumor suppressor genes involved in tuberous sclerosis complex, as regulators of the mammalian target of rapamycin (mTOR) pathway. Cells deficient in TSC1 or TSC2 possess high levels of Rheb-GTP resulting in constitutive mTOR activation. We have shown previously that the TSC1/TSC2 complex is involved in post-Golgi transport of VSVG and caveolin-1 in mammalian cells. Here, we show that modulation of mTOR activity affects caveolin-1 localization and that this effect is independent of p70S6K. Tsc1- and Tsc2-null cells exhibit abnormal caveolin-1 localization that is accompanied by disorganized microtubules in the subcortical region. Analyses of green fluorescent protein-EB1 and tubulin in live mutant cells suggest a failure of the plus-ends to sense cortical signals and to halt microtubule growth. Down-regulation of CLIP-170, a putative mTOR substrate with microtubule-binding properties, rescued the abnormal microtubule arrangement and caveolin-1 localization in Tsc2-/- cells. Together, these findings highlight a novel role of the TSC2/mTOR pathway in regulating microtubule-dependent protein transport.
Cytokine Production Patterns And Lymphoproliferative Responses In Volunteers Orally Immunized With Attenuated Vaccine Strains Of Salmonella Typhi (1994)

New recombinant strains of attenuated Salmonella typhi used as live oral vaccines elicit potent immune responses. This study examined the patterns of cytokine production and proliferation to specific S. typhi antigens in subjects orally immunized with attenuated S. typhi vaccines CVD 906, CVD 908, and CVD 908 expressing the circumsporozoite protein of Plasmodium falciparum. After immunization, sensitized lymphocytes were found in subjects' blood that exhibited significantly increased proliferative responses and interferon-gamma production to purified S. typhi flagella when compared with preimmunization levels. Significant negative correlations were observed between interleukin-4 production and both interferon-gamma production and proliferation to S. typhi flagella. These results demonstrate that oral immunization with attenuated S. typhi strains alone or with those carrying a foreign gene elicits strong systemic cell-mediated immunity to purified S. typhi antigens, including the production of cytokines compatible with T1-type responses.
Perioperative treatment of patients with obstructive sleep apnea (2004)

Obstructive sleep apnea places a significant proportion of surgical patients at increased risk of perioperative complications. Obstructive sleep apnea can be induced, unmasked, or exacerbated by the effects of sedative, analgesic, and anesthetic agents regardless of the site of surgery. The role of sleep apnea as a risk factor for development of postoperative complications needs greater emphasis. Increased awareness of the risk posed by an obstructed upper airway and appropriate management are important to optimize the perioperative care of patients with obstructive sleep apnea.
Macrophage inflammatory protein-1 alpha activates basophils and mast cells. (1992)

Macrophage inflammatory protein-1 (MIP) is a recently cloned cytokine that causes neutrophilic infiltration and induces an inflammatory response. We studied the effect of MIP-1 alpha on histamine secretion from basophils and mast cells. Leukocytes from allergic and normal subjects were studied. MIP-1 alpha caused dose-dependent release of histamine from basophils of 14 of 20 allergic donors at concentrations of 10(-9)-10(-7) M, and the mean release was 13.50 +/- 2.9% at the highest concentration. In the same experiments, the mean histamine release by anti-immunoglobulin E and monocyte chemotactic and activating factor (MCAF) (10(-7) M) was 32 +/- 7% and 31 +/- 3%, respectively. The cells from only 2 of 10 normal subjects released histamine in response to MIP-1 alpha. Histamine release by MIP-1 alpha was rapid, and almost complete within the first 3 min. MIP-1 alpha-induced degranulation was a calcium-dependent noncytotoxic process. MIP-1 alpha showed chemotactic activity for purified basophils that was comparable to MCAF. Both MIP-1 alpha and MCAF at 10(-7) M concentration elicited a chemotactic response that was 40% of the maximal response to C5a (1 microgram/ml). Murine MIP-1 alpha induced histamine release from mouse peritoneal mast cells in a dose-dependent manner. Thus, we have established that MIP-1 alpha is a novel activator of basophils and mast cells.
Experimental Study of Fracture Conductivity for Water-Fracturing and Conventional Fracturing Applications (2001)

Summary Hydraulic fracturing treatments that use treated water and very low proppant concentrations (commonly referred to as water-fracturing treatments or "waterfracs") have been successful in stimulating low-permeability reservoirs. However, the mechanism by which these treatments provide sufficient conductivity is not well understood. To understand the effects of fracture properties on conductivity, a series of laboratory conductivity experiments was performed with fractured cores from the east Texas Cotton Valley sandstone formation. The results of this study demonstrate that fracture displacement is required for surface asperities to provide residual fracture width and sufficient conductivity in the absence of proppants. However, the conductivity may vary by at least two orders of magnitude, depending on formation properties such as the degree of fracture displacement, the size and distribution of asperities, and rock mechanical properties. In the presence of proppants, the conductivity can be proppant- or asperity-dominated, depending on the proppant concentration, proppant strength, and formation properties. Under asperity-dominated conditions, the conductivity varies significantly and is difficult to predict. Low concentrations of high-strength proppant overcome the uncertainty associated with formation properties and provide proppant-dominated conductivity. The implication of these results is that the success of a water-fracturing treatment is difficult to predict because it will depend significantly on formation properties. This dependence can be overcome by using high-strength proppants or proppants at conventional field concentrations.
Functional Assays of Membrane‐Bound Proteins with SAMDI‐TOF Mass Spectrometry (2007)

Delicate probing: When nanodisc assemblies are used to present membrane proteins on a biochip surface, interactions of these proteins can be studied by mass spectrometry. The method is illustrated with the protein rhodopsin, which is immobilized to a self-assembled monolayer by way of a his-tagged membrane scaffold protein. Upon activation of rhodopsin with light, the protein complex transducin binds and can be detected using SAMDI mass spectrometry. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z702694_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.