gebhart/patrepeval huggingface dataset:
Prevalence of enteric parasites in homosexual patients attending an outpatient clinic (1986)

A total of 372 pooled stool specimens from 274 homosexual men with diarrhea were submitted for parasitologic examination over a 2.5-year period. Each two-vial pooled specimen set contained portions of stool from 3 consecutive days in Formalin and polyvinyl alcohol. Of the 274 patients, 133 (48.5%) harbored one or more intestinal protozoa, with 161 (43.3%) of the 372 specimens submitted being positive for one or more organisms. The parasites identified included Entamoeba histolytica (71 patients), Giardia lamblia (22 patients), Endolimax nana (106 patients), Entamoeba coli (39 patients), Entamoeba hartmanni (25 patients), Dientamoeba fragilis (3 patients), Iodamoeba bütschlii (2 patients), and Chilomastix mesnili (2 patients). Cryptosporidium sp. (2 patients) and Isospora belli (1 patient) were also detected. Results of this study support the experience of other workers regarding high rates of infection with intestinal parasites in the homosexual population and also indicate that symptomatic individuals belonging to this acquired immunodeficiency syndrome risk group be screened for both common and uncommon intestinal pathogens.
Resonant hemispherical dielectric antenna (1984)

An antenna consisting of a resonant dielectric hemisphere on top of a ground plane is investigated. Calculations of the internal and external fields are made with emphasis on the lowest-order mode that results in efficient radiation in the direction perpendicular to the ground plane. Measured input impedance data are also presented for a practical radiator.
Oldenlandia diffusa and Scutellaria barbata Augment Macrophage Oxidative Burst and Inhibit Tumor Growth (1996)

Oldenlandia diffusa (OD) and Scutellaria barbata (SB) have been used in traditional Chinese medicine for treating liver, lung and rectal tumors. In this study we determined the effects of these phytochemicals on oxidative burst as an indicator of phagocytic function in a murine macrophage cell line J774 using an automated micro-fluorometric assay. A dose-dependent augmentation of oxidative burst was observed with OD as well as SB. The effect of OD and SB on the growth of a murine renal cell carcinoma (Renca) was also determined. Balb/c mice were transplanted subcutaneously with 1 x 10(5) Renca cells and were randomized into groups of 10 to receive oral feeding of OD (4 mg/day), SB (4 mg/day), or saline as control. Oral feeding with either OD or SB significantly inhibited the growth of Renca in mice. The data of this study show that OD and SB are capable of enhancing macrophage function in vitro and inhibiting tumor growth in vivo.
Class-based statistical machine translation for field maintainable speech-to-speech translation (2008)

Current speech-to-speech translation systems lack any mechanism to handle out-of-vocabulary words that did not appear in the training data. To improve the usability of these systems we have developed a field maintainable speech-to-speech translation framework that enables users to add new words to the system while it is being used in the field. To realize such a framework, a novel class-based statistical machine translation framework is proposed, that applies class-based translation models and class n-gram language models during translation. To obtain consistent labelling of the parallel training corpora, on which these models are trained, we introduce a bilingual tagger that jointly labels both sides of the parallel corpora. On a Japanese-English evaluation system, the proposed framework significantly improved translation quality, obtaining a relative improvement in BLEU-score of 15% for both translation directions.
Antiretroviral Therapy in Macrophages: Implication for HIV Eradication (2009)

HIV type-1 (HIV-1) accounts for more than 25 million deaths and nearly 40 million people are infected worldwide. A significant obstacle in clearing virus from infected individuals is latently infected viral reservoirs. Latent HIV-1 can emerge with recrudescence as a productive infection later in disease progression and could provide a source for the emergence of resistant HIV-1. It is widely recognized that macrophages represent a latently infected viral reservoir and are a significant and critical HIV-1 target cell in vivo. Macrophages can be divided into multiple subsets of macrophage-like cells, all of which are susceptible to HIV-1 infection, including dendritic cells, Langerhans cells, alveolar macrophages, mucosal macrophages and microglial cells. Current antiretroviral therapy (ART) often displays differential antiviral activity in macrophages relative to CD4(+) T-lymphocytes. Significant work has been performed to establish antiviral activity of many clinically approved ART in macrophages; however, a direct link between antiviral activity and specific mechanisms responsible for these antiviral effects are incompletely understood. This review identifies many understudied areas of research, along with topics for further research in the field of HIV therapy and eradication. Discussion focuses upon the known cellular pharmacology and antiviral activity of antiretroviral agents in macrophages and its relationship to latency, chronic HIV-1 infection and therapeutic strategies to eradicate systemic HIV-1 infection.
I. Preliminary Observations on Artificial Colonization of Newborns (1963)

Glossary .—For the purpose of clarity the following terms have been defined in accord with their use in this and subsequent communications. 1-3 Interference: The inability of a second strain of coagulase positive staphylococcus to colonize a particular site of a newborn infant following artificial colonization of this specific site with staphylococcal strain 502A. This term is also used to indicate the inability of an index strain of coagulase positive staphylococci to colonize a household member who had been colonized previously with coagulase positive staphylococci. Infection: The presence of microorganisms without overt manifestations of disease. This term is equivalent to colonization. Criteria of Colonization: Isolation on artificial medium of more than one colony of a given strain of staphylococcus from a swab of the nasal mucosa or umbilicus. Colonization by a second strain is defined as the recovery of more than one colony of the second strain at any time
Use of the Humanized Anti-Epidermal Growth Factor Receptor Monoclonal Antibody h-R3 in Combination With Radiotherapy in the Treatment of Locally Advanced Head and Neck Cancer Patients (2004)

h-R3 is a well-tolerated drug that may enhance radiocurability of unresectable head and neck neoplasms.
Analysis of gene expression identifies candidate markers and pharmacological targets in prostate cancer. (2001)

Detection, treatment, and prediction of outcome for men with prostate cancer increasingly depend on a molecular understanding of tumor development and behavior. We characterized primary prostate cancer by monitoring expression levels of more than 8900 genes in normal and malignant tissues. Patterns of gene expression across tissues revealed a precise distinction between normal and tumor samples, and revealed a striking group of about 400 genes that were overexpressed in tumor tissues. We ranked these genes according to their differential expression in normal and cancer tissues by selecting for highly and specifically overexpressed genes in the majority of cancers with correspondingly low or absent expression in normal tissues. Several such genes were identified that act within a variety of biochemical pathways and encode secreted molecules with diagnostic potential, such as the secreted macrophage inhibitory cytokine, MIC-1. Other genes, such as fatty acid synthase, encode enzymes known as drug targets in other contexts, which suggests new therapeutic approaches.
Electrical stimulation of the liver cell: activation of glycogenolysis (1981)

To examine the role of ionic factors in the regulation of glycogen metabolism, we examined the effects of electrical stimulation on liver glycogen cycle enzymes. Passage of electric current through a suspension of rat hepatocytes caused the conversion of glycogen phosphorylase to its active (a) form and the simultaneous conversion of glycogen synthase to its inactive (D) form. The rise in phosphorylase a activity was dependent on the magnitude of current flow, was detectable after 5 s of current flow, and was rapidly reversible on cessation of stimulation. The activation of phosphorylase by shocking was completely eliminated by depletion of cellular Ca2+ and was restored by readdition of Ca2+. Cyclic AMP and cyclic GMP levels were unaffected by shocking. It is concluded that shocking, in the absence of any hormone or exogenous chemical, causes an increase in cytosol Ca2+, which in turn leads to activation of phosphorylase and inactivation of synthase. Electrical stimulation may serve as a model system for studying the role of ions in metabolic regulation.
A critical analysis of vector space model for information retrieval (1986)

Notations and definitions necessary to identify the concepts and relationships that are important in modelling information retrieval objects and processes in the context of vector spaces are presented. Earlier work on the use of vector model is evaluated in terms of the concepts introduced and certain problems and inconsistencies are identified. More importantly, this investigation should lead to a clear understanding of the issues and problems in using the vector space model in information retrieval. © 1986 John Wiley & Sons, Inc.
Monoclonal antibodies reveal expression of the CGRP receptor in Purkinje cells, interneurons and astrocytes of rat cerebellar cortex (1998)

The molecular layer of adult rat cerebellum displays high levels of calcitonin gene-related peptide (CGRP) receptors, but the cellular location of the receptor remains unidentified. In an attempt to reveal the expression sites of these receptors, monoclonal antibodies raised against purified CGRP receptors from porcine cerebellar membranes were used in double-immunofluorescence experiments combined with confocal microscopy. PEP-19, a marker that is highly enriched in Purkinje cells (Pc), revealed that CGRP receptors are located in Pc cytoplasm and dendrites, where they label small puncta sometimes arranged in a row along the course of the dendrite itself. CGRP receptors were also located in inhibitory interneurons. Furthermore, as shown by double-labeling experiments with GFAP, CGRP receptor-IR labeled Golgi epithelial cells and their radial fibers (Bergmann fibers), as well as astrocytic processes encircling Pc somata. The simultaneous presence of CGRP receptors in Purkinje cells and in the glial cells that heavily enshroud Purkinje cells allows us to hypothesize that these receptors may be involved in neuron-glia interactions influencing neuronal activity.
Li-Storage via Heterogeneous Reaction in Selected Binary Metal Fluorides and Oxides (2004)

We report on electrochemical reactions of a variety of metal fluorides metal oxides (TiO, NbO, and TiN with lithium in nonaqueous lithium cells over a wide voltage range (0.02-4.3 V) at room temperature. In most cases, deep Li uptake occurs via heterogeneous reaction resulting in transformation of or O or S or N) into a nanocrystalline or amorphous composite from which Li can be extracted under restoration of the phase. Thermodynamic and kinetic aspects, especially overpotential and its possible origins for both Li uptake and Li extraction processes, are discussed. © 2004 The Electrochemical Society. All rights reserved.
Tumor Necrosis Factor Receptor Superfamily 14 Is Involved in Atherogenesis by Inducing Proinflammatory Cytokines and Matrix Metalloproteinases (2001)

Tumor necrosis factor (TNF) receptor superfamily 14 (TNFRSF14) is the cellular receptor for TNF superfamily 14 (LIGHT). Immunohistochemical staining of human carotid atherosclerotic plaques revealed a high level of expression of the TNFRSF14 in regions rich in macrophages/foam cells. To investigate the role of TNFRSF14 in the functioning of monocytes in relation to atherogenesis, we have analyzed TNFRSF14 expression levels and cellular events after stimulation of TNFRSF14 in peripheral blood monocytes or the human macrophage-like cell line, THP-1. A high level of expression of TNFRSF14 was detected in activated monocytes, in macrophages derived from monocytes, and in THP-1 cells. Concomitant activation of THP-1 cells with interferon-gamma and immobilized anti-TNFRSF14 monoclonal antibody resulted in synergistic induction of proatherogenic cytokines, such as TNF-alpha and interleukin-8. Activation of THP-1 cells with immobilized anti-TNFRSF14 monoclonal antibody induced expression of matrix metalloproteinase (MMP)-1, MMP-9, MMP-13, and tissue inhibitors of metalloproteinase-1 and -2. Furthermore, immunohistochemical staining of atherosclerotic plaques with severe infiltration of foam cells revealed that the expression patterns of TNFRSF14 and MMP-1, -9, and -13 overlapped. Treatment of THP-1 cells with soluble LIGHT also caused induction of MMP-9 and interleukin-8. These data suggest that TNFRSF14 is involved in atherosclerosis via the induction of proatherogenic cytokines and decreasing plaque stability by inducing extracellular matrix-degrading enzymes.
Engineering at the Nanoscale: A Strategy for Developing High-Performance Functional Polymer Nanocomposites (2020)

The talk will concentrate on various approaches being used to engineer materials at the nanoscale for various applications in future technologies. In particular, the case of clay, carbon nanostructures (e.g., nanotubes, graphene), metal oxides, bionanomaterials (cellulose, starch, and chitin) will be used to highlight the challenges and progress. Several bio-degradable polymer systems will be considered, such as rubbers, thermoplastics, thermosets, and their blends for the fabrication of functional polymer nanocomposites. The interfacial activity of nanomaterials in compatibilism binary polymer blends will also be discussed. Various self-assembled architectures of hybrid nanostructures can be made using relatively simple processes. Some of these structures offer an excellent opportunity to probe novel nanoscale behavior and can impart unusual macroscopic end properties. I will talk about various applications of these materials, taking into account their multifunctional properties. Some of the promising applications of clay, metal oxides, nano cellulose, chitin, carbon nanomaterials, and their hybrids will be reviewed.
Circulating thrombomodulin, thrombospondin, and fibronectin in acute myeloblastic leukemias. (1999)

In this study, soluble thrombomodulin (TM) was measured as an indicator of endothelial injury in acute myelocytic leukemia (AML) together with fibronectin (FN) and thrombospondin (TS). The study group comprised of 17 (6 men, 11 women; aged 34 +/- 10 years; range 21-61 years) newly diagnosed AML patients. There was infection in 6 patients. Twelve (4 men, 8 women; aged 31 +/- 11 years; range 18-55 years) healthy subjects were studied as the control group. Plasma soluble TM levels were significantly higher in AML patients than in the healthy control group (116.6 +/- 13.7 vs 37.2 +/- 1.75 ng/ml, respectively (mean +/- SEM), p < 0.01). Plasma FN levels were found to be significantly higher in AML patients compared to the control group (15.9 +/- 2.69 vs 8.1 +/- 2.46 ng/ml, respectively (mean +/- SEM). p < 0.01 ). Plasma FN levels in infected patients were significantly lower than in non-infected patients (6.47 +/- 1.3 vs 21.0 +/- 3.1 ng/ml, respectively (mean +/- SD), p < 0.01). Plasma TS levels in the patient group were significantly lower than in the control group (20.6 +/- 1.45 vs 120.8 +/- 18.2 ng/ml, respectively (mean +/- SEM), p < 0.01). In one patient with M7 megakaryoblastic leukemia who also had a high platelet count, plasma TS levels were significantly higher than that in other patients.
The Vaccinia Virus A18R DNA Helicase Is a Postreplicative Negative Transcription Elongation Factor (1998)

Loss of vaccinia virus A18R gene function results in an aberrant transcription profile termed promiscuous transcription, defined as transcription within regions of the genome which are normally transcriptionally silent late during infection. Promiscuous transcription results in an increase in the intracellular concentration of double-stranded RNA, which in turn results in activation of the cellular 2-5A pathway and subsequent RNase L-catalyzed degradation of viral and cellular RNAs. One of three hypotheses could account for promiscuous transcription: (i) reactivation of early promoters late during infection, (ii) random transcription initiation, (iii) readthrough transcription from upstream promoters. Transcriptional analysis of several viral genes, presented here, argues strongly against the first two hypotheses. We have tested the readthrough hypothesis by conducting a detailed transcriptional analysis of a region of the vaccinia virus genome which contains three early genes (M1L, M2L, and K1L) positioned directly downstream of the intermediate gene, K2L. The results show that mutation of the A18R gene results in increased readthrough transcription of the M1L gene originating from the K2L intermediate promoter. A18R mutant infection of RNase L knockout mouse fibroblast (KO3) cells does not result in 2-5A pathway activation, yet the virus mutant is defective in late viral gene expression and remains temperature sensitive. These results demonstrate that the A18R gene product is a negative transcription elongation factor for postreplicative viral genes.
The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells (2015)

Induced pluripotent stem cells (iPS) have become crucial in medicine and biology. Several studies indicate their phenotypic similarities with cancer stem cells (CSCs) and a propensity to form tumors. Thus it is desirable to identify a trait which differentiates iPS populations and CSCs. Searching for such a feature, in this work we compare the restriction (R) point-governed regulation of cell cycle progression in different cell types (iPS, cancer, CSC and normal cells) based on the expression profile of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1). Our study reveals that PFKFB3 and PFK1 expression allows discrimination between iPS and CSCs. Moreover, cancer and iPS cells, when cultured under hypoxic conditions, alter their expression level of PFKFB3 and PFK1 to resemble those in CSCs. We also observed cell type-related differences in response to inhibition of PFKFB3. This possibility to distinguish CSC from iPS cells or non-stem cancer cells by PFKB3 and PFK1 expression improves the outlook for clinical application of stem cell-based therapies and for more precise detection of CSCs.
Evaluation techniques for storage hierarchies (1970)

Increasing speed and size demands on computer systems have resulted in corresponding demands on storage systems. Since it has been generally recognized that the speed and capacity requirements of storage systems cannot be fulfilled at an acceptable costperformance level within any single technology, storage hierarchies that use a variety of technologies have been investigated.
Classical Genetic Manipulation (1998)

A significant advantage of Pichia pastoris as an experimental system is the ability to bring to bear readily both classical and molecular genetic approaches to a research problem. Although the recent advent of yeast molecular genetics has introduced new and exciting capabilities, classical genetics remains the approach of choice in many instances. These include: the generation of mutations in previously unidentified genes (mutagenesis); the removal of unwanted secondary mutations (backcrossing); the assignment of mutations to specific genes (complementation analysis); and the construction of strains with new combinations of mutant alleles. In this chapter, these and other methods for genetic manipulation of P. pastoris are described.
Tbx2b is required for ultraviolet photoreceptor cell specification during zebrafish retinal development (2009)

The vertebrate rod and cone photoreceptors are highly specialized sensory neurons that transduce light into the chemical and electrical signals of the nervous system. Although the physiological properties of cones and rods are well known, only a handful of genes have been identified that regulate the specification of photoreceptor subtypes. Taking advantage of the mosaic organization of photoreceptors in zebrafish, we report the isolation of a mutation resulting in a unique change in photoreceptor cell fate. Mutation of the lots-of-rods (lor) locus results in a near one-for-one transformation of UV-cone precursors into rods. The transformed cells exhibit morphological characteristics and a gene-expression pattern typical of rods, but differentiate in a temporal and spatial pattern consistent with UV-cone development. In mutant larvae and adults, the highly ordered photoreceptor mosaic is maintained and degeneration is not observed, suggesting that lor functions after the specification of the other photoreceptor subtypes. In genetic chimeras, lor functions cell-autonomously in the specification of photoreceptor cell fate. Linkage analysis and genetic-complementation testing indicate that lor is an allele of tbx2b/fby (from beyond). fby was identified by a pineal complex phenotype, and carries a nonsense mutation in the T-box domain of the tbx2b transcription factor. Homozygous fby mutant larvae and lor/fby transheterozygotes also display the lots-of-rods phenotype. Based upon these data, we propose a previously undescribed function for tbx2b in photoreceptor cell precursors, to promote the UV cone fate by repressing the rod differentiation pathway.
The Parturition Defect in Steroid 5α-Reductase Type 1 Knockout Mice Is Due to Impaired Cervical Ripening (1999)

Successful delivery of the fetus (parturition) depends on coordinate interactions between the uterus and cervix. A majority (70%) of mice deficient in the type 1 isozyme of steroid 5alpha-reductase fail to deliver their young at term and thus manifest a parturition defect. Using in vitro and in vivo measurements we show here that rhythmic contractions of the uterus occur normally in these mutant mice at the end of gestation. In contrast, the cervix of the mutant animal fails to ripen at term as judged by biomechanical, histological, and endocrinological assays. Impaired metabolism of progesterone in the cervix of the mutant mice in late gestation leads to an accumulation of this steroid in the tissue. We conclude that a failure of cervical ripening underlies the parturition defect in mice lacking steroid 5alpha-reductase type 1 and that this enzyme normally plays an essential role in cervical progesterone catabolism at the end of pregnancy.
Efficacy of combined porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae vaccination in piglets (2010)

Three vaccination challenge studies were performed to evaluate the impact on vaccine efficacy of combining porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae vaccines. Piglets were vaccinated with either a M hyopneumoniae bacterin, a modified live PRRSV vaccine based on a European-type PRRSV strain, or a combination of both vaccines, followed by experimental infection with either M hyopneumoniae or PRRSV. Vaccine efficacy was evaluated by assessing lung lesion scores for M hyopneumoniae and measuring viraemia for PRRSV. There were no significant differences between the protective efficacy of the combined vaccine protocol and the protective efficacy of the two single vaccines, indicating that PRRSV vaccination did not interfere with M hyopneumoniae vaccine efficacy and vice versa.