gebhart/patrepeval huggingface dataset:
Hypoxia‐inducible factor‐1 and oncogenic signalling (2004)

An understanding of underlying mechanisms involved in the activation of HIF-1 in response to both hypoxic stress and oncogenic signals has important implications for how these processes may become deregulated in human cancer. Changes in microenvironmental stimuli such as hypoxia and growth factors in combination with genetic lesions, such as loss or inactivation of p53, PTEN or pVHL or oncogenic activation, can all lead to increased HIF-1 activity. This provides cancer cells with a distinct advantage for survival and proliferation, resulting in their ability to form vascular tumours, which are aggressive and metastatic. Accordingly, upregulation of HIF-1alpha, a key component of HIF-1, correlates with a poor treatment outcome using conventional therapies. A variety of mechanisms exist that regulate expression of HIF-1alpha. In recent years, it has become clear that an extensive network of signalling cascades converge on HIF-1alpha to regulate the transcriptional response. A better understanding of this regulation may provide a basis for the development of new cancer therapies.
6-DOF VR videos with a single 360-camera (2017)

Recent breakthroughs in consumer level virtual reality (VR) headsets are creating a growing user-base in demand for immersive, full 3D VR experiences. While monoscopic 360-videos are perhaps the most prevalent type of content for VR headsets, they lack 3D information and thus cannot be viewed with full 6 degree-of-freedom (DOF). We present an approach that addresses this limitation via a novel warping algorithm that can synthesize new views both with rotational and translational motion of the viewpoint. This enables the ability to perform VR playback of input monoscopic 360-videos files in full stereo with full 6-DOF of head motion. Our method synthesizes novel views for each eye in accordance with the 6-DOF motion of the headset. Our solution tailors standard structure-from-motion and dense reconstruction algorithms to work accurately for 360-videos and is optimized for GPUs to achieve VR frame rates (>120 fps). We demonstrate the effectiveness our approach on a variety of videos with interesting content.
Lactoperoxidase, peroxide, thiocyanate antimicrobial system: correlation of sulfhydryl oxidation with antimicrobial action (1978)

The antimicrobial activity of the lactoperoxidase, peroxide, thiocyanate system against Escherichia coli was directly related to the oxidation of bacterial sulfhydryls. Lactoperoxidase catalyzed the oxidation of thiocyanate, which resulted in the accumulation of hypothiocyanite ion, OSCN-. A portion of the bacterial sulfhydryls were oxidized by OSCN- to yield sulfenic acid and sulfenyl thiocyanate derivatives. The remaining sulfhydryls were not oxidized, although OSCN- was present in large excess. The oxidation of sulfhydryls to sulfenyl derivatives inhibited bacterial respiration. This inhibition could be reversed by adding sulfhydryl compounds to reduce the sulfenyl derivatives and the excess OSCN-. Also, this inhibition could be reversed by washing the cells so as to remove the excess unreacted OSCN-. After washing, the bacteria underwent a time-dependent recovery of their sulfhydryl content. This recovery resulted in recovery of the ability to respire. The inhibited cells were viable if diluted and plated shortly after the incubation with the lactoperoxidase, peroxide, thiocyanate system. On the other hand, long-term incubation in the presence of the excess OSCN- resulted in loss of viability. Also, the inhibition of respiration became irreversible. During this long-term incubation, the excess OSCN- was consumed and the sulfenyl derivatives disappeared.
Polo-like kinase (Plk)1 depletion induces apoptosis in cancer cells (2003)

Elevated expression of mammalian polo-like kinase (Plk)1 occurs in many different types of cancers, and Plk1 has been proposed as a novel diagnostic marker for several tumors. We used the recently developed vector-based small interfering RNA technique to specifically deplete Plk1 in cancer cells. We found that Plk1 depletion dramatically inhibited cell proliferation, decreased viability, and resulted in cell-cycle arrest with 4 N DNA content. The formation of dumbbell-like chromatin structure suggests the inability of these cells to completely separate the sister chromatids at the onset of anaphase. Plk1 depletion induced apoptosis, as indicated by the appearance of subgenomic DNA in fluorescence-activated cell-sorter (FACS) profiles, the activation of caspase 3, and the formation of fragmented nuclei. Plk1-depletion-induced apoptosis was partially reversed by cotransfection of nondegradable mouse Plk1 constructs. In addition, the p53 pathway was shown to be involved in Plk1-depletion-induced apoptosis. DNA damage occurred in Plk1-depleted cells and inhibition of ATM strongly potentiated the lethality of Plk1 depletion. Although p53 is stabilized in Plk1-depleted cells, DNA damage also occurs in p53(-/-) cells. These data support the notion that disruption of Plk1 function could be an important application in cancer therapy.
Immobilization of factor XIII on collagen membranes (1986)

Factor XIII from placenta was successfully grafted onto collagen membranes by the acyl-azide procedure. The transamidase activity retained on collagen membranes was determined by measuring the increase of fluorescence resulting from dansylcadaverine incorporation into casein. We studied the effect of different factors on the grafting: concentration and composition of the factor XIII preparation in the coupling solution and influence of the preactivation of factor XIII by thrombin. Stability studies have shown that the activity of factor XIII grafted on collagen membranes was almost constant over a period of 8 months. Sterilization by gamma-irradiation of factor XIII revealed a drastic loss of activity while the use of high-energized electron bombardment caused a reduced loss of activity. The potential of such a material for biomedical use is presently under investigation.
Expression of a Mutant Form of Cellulose Synthase AtCesA7 Causes Dominant Negative Effect on Cellulose Biosynthesis (2003)

Cellulose synthase catalytic subunits (CesAs) have been implicated in catalyzing the biosynthesis of cellulose, the major component of plant cell walls. Interactions between CesA subunits are thought to be required for normal cellulose synthesis, which suggests that incorporation of defective CesA subunits into cellulose synthase complex could potentially cause a dominant effect on cellulose synthesis. However, all CesA mutants so far reported have been shown to be recessive in terms of cellulose synthesis. In the course of studying the molecular mechanisms regulating secondary wall formation in fibers, we have found that a mutant allele of AtCesA7 gene in the fra5 (fragile fiber 5) mutant causes a semidominant phenotype in the reduction of fiber cell wall thickness and cellulose content. The fra5 missense mutation occurred in a conserved amino acid located in the second cytoplasmic domain of AtCesA7. Overexpression of the fra5 mutant cDNA in wild-type plants not only reduced secondary wall thickness and cellulose content but also decreased primary wall thickness and cell elongation. In contrast, overexpression of the fra6 mutant form of AtCesA8 did not cause any reduction in cell wall thickness and cellulose content. These results suggest that the fra5 mutant protein may interfere with the function of endogenous wild-type CesA proteins, thus resulting in a dominant negative effect on cellulose biosynthesis.
A Review of Insulated Concentric Coiled Tubing Installations for Single Well, Steam Assisted Gravity Drainage (1996)

Abstract A new recovery process which uses concentric coiled tubing has been developed to improve production capabilities in heavy oil. The process, called Single Well – Steam Assisted Gravity DrainagePatPend (SW – SAGD) relies on continuous injection of high quality steam into the toe of a horizontal well while simultaneously producing mobilized oil from the same wellbore. The well is therefore dual completed with both, production tubulars and Insulated Concentric Coiled TubingPatPend (ICCT). Having completed an extensive study into the design and fabrication of ICCT, a number of single well, SAGD installations have already been completed and are in production. This paper will review this process and will document production results from some of these early wells.
p35/p25 Is Not Essential for Tau and Cytoskeletal Pathology or Neuronal Loss in Niemann–Pick Type C Disease (2006)

Hyperactivation of the cyclin-dependent kinase 5 (cdk5), triggered by proteolytic conversion of its neuronal activator, p35, to a more potent byproduct, p25, has been implicated in Alzheimer's disease (AD), amyotrophic lateral sclerosis, and Niemann-Pick type C disease (NPC). This mechanism is thought to lead to the development of neuropathological hallmarks, i.e., hyperphosphorylated cytoskeletal proteins, neuronal inclusions, and neurodegeneration, that are common to all three diseases. This pathological ensemble is recapitulated in a single model, the npc-1 (npc(-/-)) mutant mouse. Previously, we showed that pharmacological cdk inhibitors dramatically reduced hyperphosphorylation, lesion formation, and locomotor defects in npc(-/-) mice, suggesting that cdk activity is required for NPC pathogenesis. Here, we used genetic ablation of the p35 gene to examine the specific involvement of p35, p25, and hence cdk5 activation in NPC neuropathogenesis. We found that lack of p35/p25 does not slow the onset or progression or improve the neuropathology of NPC. Our results provide direct evidence that p35/p25-mediated cdk5 deregulation is not essential for NPC pathology and suggest that similar pathology in AD may also be cdk5 independent.
Phase Separations in pH-Responsive Polyelectrolyte Multilayers:  Charge Extrusion versus Charge Expulsion (2004)

Polyelectrolyte multilayers with continuously variable amounts of ionizable weak acid functionality were prepared by blending ionizable and nonionizable polyelectrolytes in the deposition solutions. Diluting ionizable groups in this way yielded multilayers that were more structurally stable, shown by thickness and atomic force microscopy measurements, as their internal polymer charge was varied by the pH of the external solution. Multilayers prepared with opposite surface charge to that appearing within the bulk (as a result of ionization) were more stable, as were thinner films, both results suggesting the extrusion of bulk charge to the surface. These multilayers were able to control the direction and magnitude of electroosmotic flow in microfluidics systems. Multilayers bearing only one, diluted layer ofionizable material were surprisingly effective in this respect.
The thrombospondin-1 N700S polymorphism is associated with early myocardial infarction without altering von Willebrand factor multimer size (2006)

The N700S polymorphism of thrombospondin-1 (TSP-1) has been identified as a potential genetic risk factor for myocardial infarction (MI). In a large case-control study of 1425 individuals who survived a myocardial infarction prior to age 45, the N700S polymorphism was a significant risk factor for myocardial infarction in both homozygous (odds ratio [OR] 1.9, 95% confidence interval [CI] 1.1-3.3, P = .01) and heterozygous carriers of the S700 allele (OR 1.4, 95% CI 1.1-3.3, P = .01). TSP-1 has been shown to reduce von Willebrand factor (VWF) multimer size, and the domain responsible for VWF-reducing activity has been localized to the calcium-binding C-terminal sequence. As the N700S polymorphism was previously shown to alter the function of this domain, we investigated whether the altered VWF-reducing activity of TSP-1 underlies the observed prothrombotic phenotype. The TSP1 N700S polymorphism did not influence VWF multimer size in patients homozygous for either allele nor was there a significant reduction of VWF multimer size following incubation with recombinant N700S fragments or platelet-derived TSP-1.
Lenalidomide plus Dexamethasone for Relapsed or Refractory Multiple Myeloma (2007)

Lenalidomide plus dexamethasone is more effective than high-dose dexamethasone alone in relapsed or refractory multiple myeloma. (ClinicalTrials.gov number, NCT00424047 [ClinicalTrials.gov].).
Using your smartphone to detect and map heterogeneous networks and devices in the home (2014)

Heterogeneity in the wireless spectrum is an increasing problem which breaks down coordination and exacerbates wireless interference. In particular, this is a growing problem in the home: heterogeneity is increasing, yet there is a lack of tools and expertise in the home to gather the necessary information about its RF environment to combat this issue.
Inflammation in Alzheimer's disease: relevance to pathogenesis and therapy (2010)

Evidence for the involvement of inflammatory processes in the pathogenesis of Alzheimer's disease (AD) has been documented for a long time. However, the inflammation hypothesis in relation to AD pathology has emerged relatively recently. Even in this hypothesis, the inflammatory reaction is still considered to be a downstream effect of the accumulated proteins (amyloid beta (Abeta) and tau). This review aims to highlight the importance of the immune processes involved in AD pathogenesis based on the outcomes of the two major inflammation-relevant treatment strategies against AD developed and tested to date in animal studies and human clinical trials - the use of anti-inflammatory drugs and immunisation against Abeta.
A General Method to Determine the Optimal Profile of Porting Grooves in Positive Displacement Machines: the Case of External Gear Machines (2016)

In all common hydrostatic pumps, compressibility affects the commutation phases of the displacing chambers, as they switch their connection from/to the inlet to/from the outlet port, leading to pressure peaks, localized cavitation, additional port flow fluctuations and volumetric efficiency reduction. In common pumps, these effects are reduced by proper grooves that realizes gradual port area variation in proximity of these transition regions. This paper presents a method to automatically find the optimal designs of these grooves, taking as reference the case of external gear pumps. The proposed procedure does not assume a specific geometric morphology for the grooves, and it determines the best feasible designs through a multi-objective optimization procedure. A commercial gear pump is used to experimentally demonstrate the potentials of the proposed method, for a particular case aimed at reducing delivery flow oscillations.
A simple method for converting nitriles to amides. Hydrolysis with potassium hydroxide in tert-butyl alcohol (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA simple method for converting nitriles to amides. Hydrolysis with potassium hydroxide in tert-butyl alcoholJohn Herbert Hall and Matthias GislerCite this: J. Org. Chem. 1976, 41, 23, 3769–3770Publication Date (Print):November 1, 1976Publication History Published online1 May 2002Published inissue 1 November 1976https://pubs.acs.org/doi/10.1021/jo00885a032https://doi.org/10.1021/jo00885a032research-articleACS PublicationsRequest reuse permissionsArticle Views2840Altmetric-Citations93LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Increasing Prevalence and Severity of Clostridium difficile Colitis in Hospitalized Patients in the United States (2007)

The prevalence, case fatality, total mortality rate, and colectomy rate of C difficile colitis significantly increased from 1993 to 2003. These findings provide compelling evidence of the changing epidemiological features of C difficile colitis.
Plant-Produced Cottontail Rabbit Papillomavirus L1 Protein Protects against Tumor Challenge: a Proof-of-Concept Study (2006)

The native cottontail rabbit papillomavirus (CRPV) L1 capsid protein gene was expressed transgenically via Agrobacterium tumefaciens transformation and transiently via a tobacco mosaic virus (TMV) vector in Nicotiana spp. L1 protein was detected in concentrated plant extracts at concentrations up to 1.0 mg/kg in transgenic plants and up to 0.4 mg/kg in TMV-infected plants. The protein did not detectably assemble into viruslike particles; however, immunoelectron microscopy showed presumptive pentamer aggregates, and extracted protein reacted with conformation-specific and neutralizing monoclonal antibodies. Rabbits were injected with concentrated protein extract with Freund's incomplete adjuvant. All sera reacted with baculovirus-produced CRPV L1; however, they did not detectably neutralize infectivity in an in vitro assay. Vaccinated rabbits were, however, protected against wart development on subsequent challenge with live virus. This is the first evidence that a plant-derived papillomavirus vaccine is protective in an animal model and is a proof of concept for human papillomavirus vaccines produced in plants.
Trisubstituted Sulfonamides: A New Chemotype for Development of Potent and Selective CB2Receptor Inverse Agonists (2013)

An extensive exploration of the SAR of a trisubstituted sulfonamides series led to the identification of <b>39</b>, which is a potent and selective CB<sub>2</sub> receptor inverse agonist (CB<sub>2</sub><i>K</i>i = 5.4 nM, and CB<sub>1</sub><i>K</i>i = 500 nM). The functional properties measured by cAMP assays indicated that the selected compounds were CB<sub>2</sub> inverse agonists with high potency values (<b>34</b>, EC<sub>50</sub> = 8.2 nM, and <b>39</b>, EC<sub>50</sub> = 2.5 nM). Furthermore, an osteoclastogenesis bioassay indicated that trisubstituted sulfonamide compounds showed great inhibition of osteoclast formation.
Recombinant NeutraLite Avidin: a non‐glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non‐specific binding properties (2000)

A recombinant non-glycosylated and acidic form of avidin was designed and expressed in soluble form in baculovirus-infected insect cells. The mutations were based on the same principles that guided the design of the chemically and enzymatically modified avidin derivative, known as NeutraLite Avidin. In this novel recombinant avidin derivative, five out of the eight arginine residues were replaced with neutral amino acids, and two of the lysine residues were replaced by glutamic acid. In addition, the carbohydrate-bearing asparagine-17 residue was altered to an isoleucine, according to the known sequences of avidin-related genes. The resultant mutant protein, termed recombinant NeutraLite Avidin, exhibited superior properties compared to those of avidin, streptavidin and the conventional NeutraLite Avidin, prepared by chemo-enzymatic means. In this context, the recombinant mutant is a single molecular species, which possesses strong biotin-binding characteristics. Due to its acidic pI, it is relatively free from non-specific binding to DNA and cells. The recombinant NeutraLite Avidin retains seven lysines per subunit, which are available for further conjugation and derivatization.
High-performance air-gap transmission lines and inductors for millimeter-wave applications (2002)

The air-gap transmission lines and inductors are developed by new multilayer process. The developed transmission lines are air-gap coaxial line, air-gap strip line, air-gap coplanar waveguides (CPW), and air-gap buried microstrip line (BMSL). The air-gap transmission lines show very low signal loss and very high isolation performances. The transmission line loss of the coaxial line is less than 0.08 dB/mm up to 40 GHz. Those of the CPW, strip line and BMSL are about 0.07, 0.15, and 0.13 dB/mm, respectively. The isolation characteristics of the coaxial line and BMSL are measured. In case of the coaxial line with 2-mm coupling length and 60-μm distance between signal lines, the coupling is less than –52 dB up to 40 GHz. In case of the BMSL with the same conditions, the coupling is less than –43 dB. Therefore, the air-gap transmission line is very suitable structure for high performance and high-density RF application. Additionally, the air-gap inductors are monolithically fabricated using the same process of the transmission line. The fabricated inductors have very high quality factors, the maximum factor of 1.46-nH air-gap inductor is about 130. Using the developed multilayer process, we can realize various types of air-gap transmission lines (coaxial line, CPW, strip line and BMSL) and air-gap inductors simultaneously.
EDTA induces differentiation and suppresses proliferation of promyelocytic leukemia cell line HL-60--possible participation of zinc. (1992)

Effects of ethylenediaminetetraacetic acid (EDTA), a chelator of divalent cations, on the proliferation and differentiation of human promyelocytic leukemia cell line HL-60 were examined. Incubation of HL-60 cells with 200 microM of EDTA suppressed cell proliferation, and induced differentiation assessed by reductivity of nitro blue tetrazolium and activity of alpha-naphthyl butyrate esterase. These effects were inhibited by zinc (Zn) dose-dependently at concentrations of up to 20 microM, but not by other divalent cations including Ca, Mg, Fe, Cu, Ni, or Cd. Although addition of 200 microM EDTA for 24 hr decreased the c-myc mRNA level of HL-60 cells, coaddition of 20 microM of Zn also reversed this effect on c-myc mRNA level. Treatment with EDTA did not change the half-life time of degradation of c-myc mRNA after addition of actinomycin D (5 micrograms/ml). These findings suggest that Zn deficiency suppresses c-myc gene transcription which is followed by suppression of proliferation and induction of differentiation of HL-60 cells.
Structural remodelling in heart failure: gelatinase induction. (1994)

The association of 92 kDa gelatinase with changes in left ventricular area suggests a possible modulating role for this matrix metalloproteinase in disruption of the fibrillar components of the left ventricular extracellular matrix.
Ultralow Hysteresis Superhydrophobic Surfaces by Excimer Laser Modification of SU-8 (2006)

We present a new and simple method to produce superhydrophobic surfaces with ultralow hysteresis. The method involves surface modification of SU-8 using an excimer laser treatment. The modified surface is coated with a hydrophobic plasma-polymerized hexafluoropropene layer. The advancing and receding water contact angles were measured to be approximately 165 degrees . The achieved water contact angle hysteresis was below the measurement limit. This low hysteresis can be ascribed to nanoscale debris generated during the excimer laser process.