gebhart/patrepeval huggingface dataset:
Nitrogen Fertilization Timing Effect on Wheat Production, Nitrogen Uptake Efficiency, and Residual Soil Nitrogen (1993)

Abstract Split fertilizer N application has been proposed for improving N uptake efficiency in wheat ( Triticum aestivum L.) production systems, but results have been inconsistent. In this field study, 75 and 150 kg N ha −1 was applied either all preplant or in two, three, or four split applications based on wheat growth stage (GS) to determine N timing effect on wheat yield and apparent fertilizer N recovery. Vegetative samples were also collected at Feekes GS 4, 6, and 10 to determine treatment effects on tissue N concentration during the growing season. Soil samples were collected after harvest to evaluate residual N quantity and movement in soil relative to N rate and time of application. The soil used was a Fluventic Ustochrept‐Udic Chromustert intergrade. Nitrogen rate and application timing appeared to have less effect than year on yield and N parameters. Significant grain yield increases were achieved with split applications of N fertilizer when N was topdressed at GS 4 or 6 in 1989 as compared with all preplant or application at GS 10. Split N application at GS 10 produced greater grain yield than application at GS 4 or 6 in 1990. Nitrogen uptake efficiency was greatest with 75 kg N ha −1 and split application. Soil NO ‐ 3 –N concentration was significantly higher at 150 kg N ha −1 than at 75 kg N ha −1 . Significantly lower residual NO ‐ 3 ‐N with the preplant treatment was not accounted for by greater grain or straw N content.
Selective carboxymethylation of the alpha-amino groups of hemoglobin. Effect on functional properties. (1983)

Hemoglobin A hybrids with carboxymethyl groups at the alpha-NH2 termini of the alpha-chains or the beta-chains or at the termini of both chains have been prepared by reductive alkylation of the protein with glyoxylate and NaCNBH3 under controlled conditions. A hemoglobin derivative, which was selectively carboxymethylated at the NH2-terminal residues, was separated into its alpha- and beta-chains. These derivatized chains were recombined to yield alpha 2 Cm beta 2 Cm or were combined with unmodified beta- or alpha-chains, respectively, and purified to yield alpha 2 Cm beta 2 or alpha 2 beta 2 Cm. These hybrid tetramers retained their cooperativity and function (average n = 2.4). The hybrid alpha 2 Cm beta 2 had a lower oxygen affinity (p50 = 12 mm) than unmodified hemoglobin (p50 = 7 mm) and was reactive with 2,3-diphosphoglycerate (p50 = 48 mm). The oxygen affinity of the derivative alpha 2 beta 2 Cm was lower (p50 = 17 mm) than unmodified hemoglobin and was affected only slightly by 2,3-diphosphoglycerate (p50 = 25 mm). The tetramer carboxymethylated at all 4 NH2-terminal residues, alpha 2 Cm beta 2 Cm, exhibited a very low intrinsic oxygen affinity (p50 = 37 mm) that was further lowered to a limiting value of 50 mm by saturating amounts of 2,3-diphosphoglycerate. Each carboxymethyl tetramer, except alpha 2 Cm beta 2 Cm, was reactive with chloride to lead to a lower oxygen affinity. These carboxymethylated hybrids (Hb-NH-CH2COO-) may provide a useful model system for studies on the binding of anions to hemoglobin or on the interaction of CO2 with hemoglobin to form the carbamate Hb-NH-COO-.
Evaluation of radiolabelled bombesin analogues for receptor-targeted scintigraphy and radiotherapy (1999)

The 14-aminoacid peptide bombesin (BN) has a high affinity for the gastrin-releasing peptide receptor which is expressed by a variety of tumours. Thus, radiometal-labelled DTPA-BN derivatives are potentially useful radioligands for receptor-targeted scintigraphy and radiotherapy of BN receptor-expressing tumours. A number of such DTPA-BN analogues, [DTPA-D-Tyr6]BN(6-13)NHEt (Et=ethyl), [DTPA-Tyr5,D-Phe6]BN(5-13)NHEt, [DTPA-D-Phe6,Leu13psiPhe14]BN(6-14), [DTPA-Tyr5,D-Phe6,Leu13psiPhe14]BN(5-14), [DTPA-Pro1,Tyr4]BN and [DTPA-Pro1,Tyr4,Nle14]BN, were synthesized and studied for their binding characteristics to the BN receptor on 7315b rat pituitary tumour cell membranes in competition with [125I-Tyr4]BN. The effects of the BN analogues were determined on basal and BN-stimulated prolactin secretion by 7315b cells to distinguish between their agonistic and antagonistic characteristics. Internalization of selected (111)In-labelled BN analogues was studied using the BN receptor-positive 7315b pituitary tumour and the CA20948 and AR42J exocrine pancreas tumour cell lines. The tissue distribution of these (111)In-labelled BN analogues was investigated in 7315b tumour-bearing rats. Two DTPA-conjugated analogues, the antagonist [DTPA-Tyr5,D-Phe6]BN(5-13)NHEt and the agonist [DTPA-Pro1,Tyr4]BN showed the highest affinity for the BN receptor on 7315b cell membranes. Despite similar affinity for the BN receptor, the (111)In-labelled agonist, but not the antagonist, was internalized by the BN receptor-positive tumour cells. Consonant with this observation, the agonist [(111)In-DTPA-Pro1,Tyr4]BN showed much higher specific uptake in BN receptor-positive tissues and tumour than the antagonist [(111)In-DTPA-Tyr5,D-Phe6]BN(5-13)NHEt, with concordant target to background ratios. We conclude that [(111)In-DTPA-Pro1,Tyr4]BN has promising characteristics for applications in nuclear medicine.
OASIS/CREB3L1 Is Induced by Endoplasmic Reticulum Stress in Human Glioma Cell Lines and Contributes to the Unfolded Protein Response, Extracellular Matrix Production and Cell Migration (2013)

OASIS is a transcription factor similar to ATF6 that is activated by endoplasmic reticulum stress. In this study we investigated the expression of OASIS in human glioma cell lines and the effect of OASIS knock-down on the ER stress response and cell migration. OASIS mRNA was detected in three distinct glioma cell lines (U373, A172 and U87) and expression levels were increased upon treatment with ER stress-inducing compounds in the U373 and U87 lines. OASIS protein, which is glycosylated on Asn-513, was detected in the U373 and U87 glioma lines at low levels in control cells and protein expression was induced by ER stress. Knock-down of OASIS in human glioma cell lines resulted in an attenuated unfolded protein response to ER stress (reduced GRP78/BiP and GRP94 induction) and decreased expression of chondroitin sulfate proteoglycan extracellular matrix proteins, but induction of the collagen gene Col1a1 was unaffected. Cells in which OASIS was knocked-down exhibited altered cell morphology and reduced cell migration. These results suggest that OASIS is important for the ER stress response and maintenance of some extracellular matrix proteins in human glioma cells.
Lifetime Comorbidity of Panic Attacks and Major Depression in a Population-Based Study (1994)

These findings may indicate a worse severity when the two disorders occur in the same individual.
Using NUMA interconnects for highly available filers (1999)

The paper discusses the clustered failover configuration which connects two independent file-server appliances via a nonuniform-memory-access network. Combining NUMA interconnects and a proprietary, log-structured file system results in file service that survives hardware faults with minimal disruption to clients.
The Metabolism of Iron‐Dextran Given as a Total‐Dose Infusion to Iron Deficient Jamaican Subjects* (1968)

S ummary The movements of iron‐dextran, following a total dose infusion of up to 2550 mg. iron in six severely iron deficient patients, have been studied using a 59 Fe tagged complex. The preparation is removed from the plasma in 8–10 days. Fifty per cent of the iron was incorporated into haemoglobin in 3–4 weeks and the iron stores replenished rapidly. Using organ scanning, the liver was confirmed as the site of primary plasma clearance, but accumulations of iron were also detected in the spleen and bone marrow. Only minute quantities of radioactivity appeared in the urine. No untoward effects on iron metabolism have been detected, but reduction of the serum iron and total iron binding capacity to low levels 6 weeks post infusion is of interest. An attempt has been made to interpret the findings to elucidate the ferrokinetics of this method of treatment.
Accurate estimation of homologue-specific DNA concentration-ratios in cancer samples allows long-range haplotyping (2011)

Abstract Interpretation of allelic copy measurements at polymorphic markers in cancer samples presents distinctive challenges and opportunities. Due to frequent gross chromosomal alterations occurring in cancer (aneuploidy), many genomic regions are present at homologous-allele imbalance. Within such regions, the unequal contribution of alleles at heterozygous markers allows for direct phasing of the haplotype derived from each individual parent. In addition, genome-wide estimates of homologue specific copy- ratios (HSCRs) are important for interpretation of the cancer genome in terms of fixed integral copy-numbers. We describe HAPSEG, a probabilistic method to interpret bi- allelic marker data in cancer samples. HAPSEG operates by partitioning the genome into segments of distinct copy number and modeling the four distinct genotypes in each segment. We describe general methods for fitting these models to data which are suit- able for both SNP microarrays and massively parallel sequencing data. In addition, we demonstrate a specially tailored error-model for interpretation of systematic variations arising in microarray platforms. The ability to directly determine haplotypes from cancer samples represents an opportunity to expand reference panels of phased chromosomes, which may have general interest in various population genetic applications. In addition, this property may be exploited to interrogate the relationship between germline risk and cancer phenotype with greater sensitivity than is possible using unphased genotype. Finally, we exploit the statistical dependency of phased genotypes to enable the fitting of more elaborate sample-level error-model parameters, allowing more accurate estimation of HSCRs in cancer samples.
Effects of curcumin on retinal oxidative stress and inflammation in diabetes (2007)

Thus, the beneficial effects of curcumin on the metabolic abnormalities postulated to be important in the development of diabetic retinopathy suggest that curcumin could have potential benefits in inhibiting the development of retinopathy in diabetic patients.
Background limited long wavelength infrared type-II InAs/GaSb superlattice photodiodes operating at 110 K (2008)

The utilization of the P+-π-M-N+ photodiode architecture in conjunction with a thick active region can significantly improve long wavelength infrared type-II InAs/GaSb superlattice photodiodes. By studying the effect of the depletion region placement on the quantum efficiency in a thick structure, we achieved a topside illuminated quantum efficiency of 50% for an N-on-P diode at 8.0 μm at 77 K. Both the double heterostructure design and the application of polyimide passivation greatly reduce the surface leakage, giving an R0A of 416 Ω cm2 for a 1% cutoff wavelength of 10.52 μm, a Shot–Johnson detectivity of 8.1×1011 cmHz/W at 77 K, and a background limited operating temperature of 110 K with 300 K background.
ChemInform Abstract: Catalytic Asymmetric Synthesis of Peptides on Polymer Support (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Two Sensor Kinases Contribute to the Hypoxic Response of Mycobacterium tuberculosis (2004)

Current estimates indicate that nearly a third of the world's population is latently infected with Mycobacterium tuberculosis. Reduced oxygen tension and nitric oxide exposure are two conditions encountered by bacilli in vivo that may promote latency. In vitro exposure to hypoxia or nitric oxide results in bacterial stasis with concomitant induction of a 47-gene regulon controlled by the transcription factor DosR. In this report we demonstrate that both the dosS gene adjacent to dosR and another gene, dosT (Rv2027c), encode sensor kinases, each of which can autophosphorylate at a conserved histidine and then transfer phosphate to an aspartate residue of DosR. Mutant bacteria lacking both sensors are unable to activate expression of DosR-regulated genes. These data indicate that DosR/DosS/DosT comprise a two-component signaling system that is required for the M. tuberculosis genetic response to hypoxia and nitric oxide, two conditions that produce reversible growth arrest in vitro and may contribute to latency in vivo.
Sagittal plane biomechanics. American Diabetes Association (2000)

During walking, the center of body mass must pass from behind the weightbearing foot to in front of it. For this to take place, the foot must function as a sagittal plane pivot. Because the range required for this motion is approximately five times as great as both frontal and transverse plane motion, its evaluation should become an essential part of a podiatric biomechanical assessment. Lack of proper sagittal plane motion and its sequelae are described.
Codecast: a network-coding-based ad hoc multicast protocol (2006)

In this article we present CodeCast, a network-coding-based ad hoc multicast protocol. CodeCast is especially well-suited for multimedia applications with low-loss, low-latency constraints such as audio/video streaming. The key ingredient of CodeCast is random network coding, which transparently implements both localized loss recovery and path diversity with very low overhead. Simulation results show that in a typical setting, CodeCast yields a nearly 100 percent delivery ratio, as compared to a 94 percent delivery ratio by traditional multicast. More importantly, the overhead is reduced by as much as 50 percent
Purification and characterization of trichosanthin. Homology to the ricin A chain and implications as to mechanism of abortifacient activity. (1987)

Trichosanthin, a protein from the Chinese medicinal herb Trichosanthes kirilowii, was purified in two essentially quantitative steps involving CM-Sephadex chromatography and reverse-phase high performance liquid chromatography. The protein was found to have a molecular mass of 25-26 kDa, to contain no cysteine, and to contain no glycosidic linkages. Pure trichosanthin was found to have potent abortifacient activity in pregnant mice. In order to understand the molecular basis of this unique biological activity, we have examined the amino acid sequence of the protein. As purified, trichosanthin was found to contain two amino-terminal sequences which differed only in the absence or presence of a tyrosine at residue 1. Sequence analysis of trichosanthin has allowed for determination of the NH2-terminal 38-amino acid residues. Comparison of this sequence to those present in a data base revealed homology with the ricin A-chain. Consistent with this structural homology, we have found that trichosanthin is a potent inhibitor of protein synthesis in a reticulocyte lysate system.
Preliminary Design and Experimental Studies of a Novel Soft Implant for Correcting Sagittal Plane Instability in the Lumbar Spine (1997)

A circular silicone spacer placed between the spinous processes appears to contribute to the stability of the cadaveric lumbar spine. There are many attractions to using a simple, soft implant that can be placed with minimal surgery between the spinous processes.
Quantifying EGFR Alterations in the Lung Cancer Genome with Nanofluidic Digital PCR Arrays (2010)

The digital PCR array platform allows characterization and quantification of oncogenes, such as EGFR, at the single-molecule level. Use of this nanofluidics platform may provide deeper insight into the specific roles of clinically relevant kinase mutations during different stages of lung tumor progression and may be useful in predicting the clinical response to EGFR-targeted inhibitors.
Bioartificial Skin (2000)

The loss of skin has been one of the oldest, yet most frequent and costly problems in our health care system. To restore functional and esthetic integrity in patients with unstable or hypertrophic scars, in burn patients and after skin loss for hereditary, traumatic or oncological reasons, an armamentarium of reconstructive surgical procedures including autogenous, allogenous and xenogenous tissue transfer as well as implantation of alloplastic materials has been favored. For several decades there has been increasing interest focused on 'tissue engineering' of dermal, epidermal and full thickness skin substitutes by both biological and synthetic matrices. At our institution (Hannover Medical School), a collagen/glycosaminoglycan dermal regeneration matrix has been used for immediate dermal coverage after escharectomy in burn injuries as well as for dermal replacement in chronically unstable scars. This article gives an overview on the current state of the art in bioartificial skin as well as our personal experience with the collagen/glycosaminoglycan matrix for dermal replacement in different clinical situations.
Gene Therapy for Amyotrophic Lateral Sclerosis and Other Motor Neuron Diseases (2000)

There are several incurable diseases of motor neuron degeneration, including amyotrophic lateral sclerosis (ALS), primary lateral sclerosis, hereditary spastic hemiplegia, spinal muscular atrophy, and bulbospinal atrophy. Advances in gene transfer techniques coupled with new insights into molecular pathology have opened promising avenues for gene therapy aimed at halting disease progression. Nonviral preparations and recombinant adenoviruses, adeno-associated viruses, herpesviruses, and lentiviruses may ultimately transduce sufficient numbers of cerebral, brainstem, and spinal cord neurons for therapeutic applications. This could be accomplished by direct injection, transduction of lower motor neurons via retrograde transport after intramuscular injection, or cell-based therapies. Studies using transgenic mice expressing mutant superoxide dismutase 1 (SOD1), a model for one form of ALS, established that several proteins were neuroprotective, including calbindin, bcl-2, and growth factors. These same molecules promoted neuronal survival in other injury models, suggesting general applicability to all forms of ALS. Potentially correctable genetic lesions have also been identified for hereditary spastic hemiplegia, bulbospinal atrophy, and spinal muscular atrophy. Finally, it may be possible to repopulate lost corticospinal and lower motor neurons by transplanting stem cells or stimulating native progenitor populations. The challenge ahead is to translate these basic science breakthroughs into workable clinical practice.
Linear stability analysis of capillary instabilities for concentric cylindrical shells (2011)

Abstract Motivated by complex multi-fluid geometries currently being explored in fibre-device manufacturing, we study capillary instabilities in concentric cylindrical flows of $N$ fluids with arbitrary viscosities, thicknesses, densities, and surface tensions in both the Stokes regime and for the full Navier–Stokes problem. Generalizing previous work by Tomotika ( $N= 2$ ), Stone &amp; Brenner ( $N= 3$ , equal viscosities) and others, we present a full linear stability analysis of the growth modes and rates, reducing the system to a linear generalized eigenproblem in the Stokes case. Furthermore, we demonstrate by Plateau-style geometrical arguments that only axisymmetric instabilities need be considered. We show that the $N= 3$ case is already sufficient to obtain several interesting phenomena: limiting cases of thin shells or low shell viscosity that reduce to $N= 2$ problems, and a system with competing breakup processes at very different length scales. The latter is demonstrated with full three-dimensional Stokes-flow simulations. Many $N\gt 3$ cases remain to be explored, and as a first step we discuss two illustrative $N\ensuremath{\rightarrow} \infty $ cases, an alternating-layer structure and a geometry with a continuously varying viscosity.
The relation of RNA and protein synthesis to the sedimentation of muscle ribosomes: effect of diabetes and insulin. (1967)

Ribosomes from muscle of alloxan-diabetic rats fuiietion less efficiently in the synthesis of protein than ribosomes from niormal aniimals.1-4 The nature of the change in function is unknowni. The decrease in protein synthesis is not due to a deficiency in the amount of messenger RNA, but rather the result of a defect in its utilization or translation by ribosomes. 1 2 An important unresolved problem is the nature of the putative chanige in the ribosomes that leads to an alteration in the translation of messenger RNA. It is our hope that a study of the chemical and physical properties of the ribonucleoprotein particles from muscle of diabetic animals will provide a solution to the problem. We have begun the study with an examination of the sedimentationi of muscle ribosomes and of the relation of RNA and. protein synthesis to the influence of diabetes and insulin on those properties.
5'-O-Monophosphoryladenylyl(2' goes to 5')adenylyl(2' goes to 5')adenosine is an antagonist of the action of 5'-O-triphosphoryladenylyl-(2' goes to 5')adenylyl(2' goes to 5')adenosine and double-stranded RNA. (1981)

5'-O-Monophosphoryladenylyl(2' goes to 5')adenylyl-(2' goes to 5')adenosine [(2' goes to 5')(pA)3] antagonizes the protein synthesis inhibitory effects of 5'-O-triphosphoryladenylyl-(2' goes to 5')adenylyl(2' goes to 5')adenosine by preventing activation of the (2' goes to 5')oligo(a)-activated ribonuclease which degrades mRNA. The oligoribonucleotide (2' goes to 5')(pA)3 also antagonizes the translational inhibitory capacity of poly(I).poly(C) in extracts of interferon-treated L cells, suggesting that (2' goes to 5')oligo(A) is the primary mediator of the protein synthesis inhibitory effects of poly(I).poly(C) in this cell-free system.
Visualization and Virtual Screening of the Chemical Universe Database GDB-17 (2012)

The chemical universe database GDB-17 contains 166.4 billion molecules of up to 17 atoms of C, N, O, S, and halogens obeying rules for chemical stability, synthetic feasibility, and medicinal chemistry. GDB-17 was analyzed using 42 integer value descriptors of molecular structure which we term "Molecular Quantum Numbers" (MQN). Principal component analysis and representation of the (PC1, PC2)-plane provided a graphical overview of the GDB-17 chemical space. Rapid ligand-based virtual screening (LBVS) of GDB-17 using the city-block distance CBD(MQN) as a similarity search measure was enabled by a hashed MQN-fingerprint. LBVS of the entire GDB-17 and of selected subsets identified shape similar, scaffold hopping analogs (ROCS > 1.6 and T(SF) < 0.5) of 15 drugs. Over 97% of these analogs occurred within CBD(MQN) ≤ 12 from each drug, a constraint which might help focus advanced virtual screening. An MQN-searchable 50 million subset of GDB-17 is publicly available at www.gdb.unibe.ch .