gebhart/patrepeval huggingface dataset:
A Stereotactic Method of Anatomical Localization for Positron Emission Tomography (1985)

Extant methods for anatomical localization within a physiological image are inadequate for precise regional correlations between anatomy and physiology. We have developed a method for determining the location of any region of interest within a positron emission tomographic (PET) image by transformation of the tomographic location coordinates into coordinates within a stereotactic atlas of the human brain. The specific anatomical assumptions of this approach were identified and validated within groups of normal subjects by means of measurements made from lateral skull radiographs and nuclear magnetic resonance proton images. A high degree of accuracy and reproducibility was demonstrated when this technique was applied to groups of normal subjects by determining the anatomical location of two physiologically defined PET areas: the cavernous sinus and visually responsive cortex. This method is simple to implement and highly objective. Generalization of the localization procedure for use with structural tomography is readily accomplished.
Anaplerosis from heptanoate ‐a propionyl‐CoA precursor‐ in mouse brain (2007)

Dietary therapy with triheptanoin, a precursor of anaplerotic propionyl‐CoA, is used for the chronic treatment of long‐chain fatty acid oxidation disorders (Roe, JCI 110 : , 2002) and pyruvate carboxylase deficiency (Mochel, Mol Gen Metab 84 : , 2005). Heptanoate is partially converted in liver to C 5 ‐ketone bodies (β‐ketopentanoate and β‐hydroxypentanoate. These are precursors of propionyl‐CoA in peripheral tissues. We tested whether heptanoate can be taken up as such by the brain. Anesthetized mice were infused with [5,6,7‐ 13 C 3 ]heptanoate (20 to 100 nmol/g x min) for 30 min before freezing the brains. We assayed the concentration and mass isotopomer distribution of brain acyl‐CoAs by LC‐MS‐MS. We found that the brain concentrations of [5,6,7‐ 13 C 3 ]heptanoyl‐CoA, [3,4,5‐ 13 C 3 ]pentanoyl‐CoA, and [1,2,3‐ 13 C 3 ]propionyl‐CoA increased with the rate of infusion of [5,6,7‐ 13 C 3 ]heptanoate. The spectrum of triply‐labeled acyl‐CoAs demonstrates that heptanoate is taken up as such by the brain, and that it is anaplerotic in this organ. Our data support the hypothesis, presented in ( Mol Gen Metab 84 : , 2005) that the neurological status of a patient with pyruvate carboxylase deficiency was improved by anaplerosis from heptanoate in the brain. These investigations are extended to mice deficient in very long‐chain acyl‐CoA dehydrogenase deficiency. Supported by NIH grant DK069752.
Critical roles of the guanylyl cyclase B receptor in endochondral ossification and development of female reproductive organs (2004)

Guanylyl cyclase B is the receptor for a small peptide (C-type natriuretic peptide) produced locally in many different tissues. To unravel the functions of the receptor, we generated mice lacking guanylyl cyclase B through gene targeting. Expression of the receptor mRNA in tissues such as bone and female reproductive organs was evident, and significant phenotypes associated with each of these tissues were apparent in null mice. A dramatic impairment of endochondral ossification and an attenuation of longitudinal vertebra or limb-bone growth were seen in null animals. C-type natriuretic peptide-dependent increases of guanylyl cyclase B activity, but not basal enzyme activity, appeared to be required for the progression of endochondral ossification. Female mice were infertile, but male mice were not. This result was due to the failure of the female reproductive tract to develop. Thus, the guanylyl cyclase B receptor is critical for the development of both bone and female reproductive organs.
Mutational analysis of a ras catalytic domain. (1986)

We used linker insertion-deletion mutagenesis to study the catalytic domain of the Harvey murine sarcoma virus v-rasH transforming protein, which is closely related to the cellular rasH protein. The mutants displayed a wide range of in vitro biological activity, from those that induced focal transformation of NIH 3T3 cells with approximately the same efficiency as the wild-type v-rasH gene to those that failed to induce any detectable morphologic changes. Correlation of transforming activity with the location of the mutations enabled us to identify three nonoverlapping segments within the catalytic domain that were dispensable for transformation and six other segments that were required for transformation. Segments that were necessary for guanosine nucleotide (GDP) binding corresponded to three of the segments that were essential for transformation; two of the three segments share strong sequence homology with other purine nucleotide-binding proteins. Loss of GDP binding was associated with apparent instability of the protein. Lesions in two of the three other required regions significantly reduced GDP binding, while small lesions in the last required region did not impair GDP binding or membrane localization. We speculate that this latter region interacts with the putative cellular target of ras. The results suggest that transforming ras proteins require membrane localization, guanosine nucleotide binding, and an additional undefined function that may represent interaction with their target.
Fluorescence and Redox Activity of Probes Anchored through an Aminotrithiol to Polycrystalline Gold (1998)

A self-assembled monolayer of tris[3-(mercaptopropyl)methyl)]-N-(aminoacetyl)amine (1) covalently anchored onto a polycrystalline gold surface acts as an effective linking agent for binding surface probes that can be activated by light or by an applied potential. Fluorescence from two appended chromophores was observed when scattering of incident light from the gold support was minimized. Monolayers of 1 coupled to an end-terminated ferrocenyl group showed blocking electrochemical behavior by cyclic voltammetry, while retaining the characteristic redox activity of the attached group. These results indicate high yields of in situ surface coupling and significant local order within the two-dimensional array.
Nrf2 and Keap1 Abnormalities in Non–Small Cell Lung Carcinoma and Association with Clinicopathologic Features (2010)

Increased expression of Nrf2 and decreased expression of Keap1 are common abnormalities in NSCLC and are associated with a poor outcome. Nuclear expression of Nrf2 in malignant lung cancer cells may play a role in resistance to platinum-based treatment in squamous cell carcinoma.
Exact Routing in Large Road Networks Using Contraction Hierarchies (2012)

Contraction hierarchies are a simple approach for fast routing in road networks. Our algorithm calculates exact shortest paths and handles road networks of whole continents. During a preprocessing step, we exploit the inherent hierarchical structure of road networks by adding shortcut edges. A subsequent modified bidirectional Dijkstra algorithm can then find a shortest path in a fraction of a millisecond, visiting only a few hundred nodes. This small search space makes it suitable to implement it on a mobile device. We present a mobile implementation that also handles changes in the road network, like traffic jams, and that allows instantaneous routing without noticeable delay for the user. Also, an algorithm to calculate large distance tables is currently the fastest if based on contraction hierarchies.
Staphylococcal pathogenicity island interference with helper phage reproduction is a paradigm of molecular parasitism (2012)

Staphylococcal pathogenicity islands (SaPIs) carry superantigen and resistance genes and are extremely widespread in Staphylococcus aureus and in other Gram-positive bacteria. SaPIs represent a major source of intrageneric horizontal gene transfer and a stealth conduit for intergeneric gene transfer; they are phage satellites that exploit the life cycle of their temperate helper phages with elegant precision to enable their rapid replication and promiscuous spread. SaPIs also interfere with helper phage reproduction, blocking plaque formation, sharply reducing burst size and enhancing the survival of host cells following phage infection. Here, we show that SaPIs use several different strategies for phage interference, presumably the result of convergent evolution. One strategy, not described previously in the bacteriophage microcosm, involves a SaPI-encoded protein that directly and specifically interferes with phage DNA packaging by blocking the phage terminase small subunit. Another strategy involves interference with phage reproduction by diversion of the vast majority of virion proteins to the formation of SaPI-specific small infectious particles. Several SaPIs use both of these strategies, and at least one uses neither but possesses a third. Our studies illuminate a key feature of the evolutionary strategy of these mobile genetic elements, in addition to their carriage of important genes-interference with helper phage reproduction, which could ensure their transferability and long-term persistence.
An Investigation of the Metabolism of Isoleucine to Active Amyl Alcohol in Saccharomyces cerevisiae (2000)

The metabolism of isoleucine to active amyl alcohol (2-methylbutanol) in yeast was examined by the use of (13)C nuclear magnetic resonance spectroscopy, combined gas chromatography-mass spectrometry, and a variety of mutants. From the identified metabolites a number of routes between isoleucine and active amyl alcohol seemed possible. All involved the initial decarboxylation of isoleucine to alpha-keto-beta-methylvalerate. The first, via branched chain alpha-ketoacid dehydrogenase to alpha-methylbutyryl-CoA, was eliminated because abolition of branched-chain alpha-ketoacid dehydrogenase in an lpd1 disruption mutant did not prevent the formation of active amyl alcohol. However, the lpd1 mutant still produced large amounts of alpha-methylbutyrate which initially seemed contradictory because it had been assumed that alpha-methylbutyrate was derived from alpha-methylbutyryl-CoA via acyl-CoA hydrolase. Subsequently it was observed that alpha-methylbutyrate arises from the non-enzymic oxidation of alpha-methylbutyraldehyde (the immediate decarboxylation product of alpha-keto-beta-methylvalerate). Mutant studies showed that one of the decarboxylases encoded by PDC1, PDC5, PDC6, YDL080c, or YDR380w must be present to allow yeast to utilize alpha-keto-beta-methylvalerate. Apparently, any one of this family of decarboxylases is sufficient to allow the catabolism of isoleucine to active amyl alcohol. This is the first demonstration of a role for the gene product of YDR380w, and it also shows that the decarboxylation steps for each alpha-keto acid in the catabolic pathways of leucine, valine, and isoleucine are accomplished in subtly different ways. In leucine catabolism, the enzyme encoded by YDL080c is solely responsible for the decarboxylation of alpha-ketoisocaproate, whereas in valine catabolism any one of the isozymes of pyruvate decarboxylase will decarboxylate alpha-ketoisovalerate.
Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex (1996)

p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox. Although its function is not well defined, preliminary evidence indicates that it is a component of the cytosolic complex. We report the characterization of the human p40-phox gene, which is single copy and spans approximately 18 kb with 10 exons. Based on fluorescent in situ hybridization (FISH) studies and analysis of somatic hybrid cell lines, the chromosomal location of p40-phox is human chromosome 22q13.1. The start of transcription has been mapped to bp -156. The expression of p40-phox message is restricted to hematopoietic cells. In addition to identifying the mRNA transcript on Northern blot analysis in cells known to express components of the NADPH-oxidase, polymorphonuclear leukocytes, monocytes, B lymphoblastoid cell lines, and eosinophils, p40-phox is also expressed in two other cell types of white cell lineage, mast cells, and basophils. In addition, the mRNA for p40-phox is expressed in megakaryocytic cells, but not in erythroid cells.
Discrimination in Intestinal Absorption of Strontium and Calcium. (1961)

Absorption of Sr85 and Ca45 was studied in the rat by the technic of in vivo intestinal perfusion. With no calcium added to the perfusion solution the percentage of Sr85 absorbed was about 0.3 that of Ca45. With increasing levels of calcium in the perfusion solution, the percentage of Sr85 and Ca45 absorbed decreased; however, the ratio of percentage absorption of Sr85 to Ca45 increased, reaching a value of about 0.6 in 25 mM calcium solution. There was no evidence of discrimination in the reverse movement of Sr85 and Ca45 from blood to intestine. The discrimination which occurs in the absorption process would appear to be a dominant factor in determining over-all strontium calcium relationships.
Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase (2014)

NUAK1 (NUAK family SnF1-like kinase-1) and NUAK2 protein kinases are activated by the LKB1 tumour suppressor and have been implicated in regulating multiple processes such as cell survival, senescence, adhesion and polarity. In the present paper we present evidence that expression of NUAK1 is controlled by CDK (cyclin-dependent kinase), PLK (Polo kinase) and the SCFβTrCP (Skp, Cullin and F-boxβTrCP) E3 ubiquitin ligase complex. Our data indicate that CDK phosphorylates NUAK1 at Ser445, triggering binding to PLK, which subsequently phosphorylates NUAK1 at two conserved non-catalytic serine residues (Ser476 and Ser480). This induces binding of NUAK1 to βTrCP, the substrate-recognition subunit of the SCFβTrCP E3 ligase, resulting in NUAK1 becoming ubiquitylated and degraded. We also show that NUAK1 and PLK1 are reciprocally controlled in the cell cycle. In G2-M-phase, when PLK1 is most active, NUAK1 levels are low and vice versa in S-phase, when PLK1 expression is low, NUAK1 is more highly expressed. Moreover, NUAK1 inhibitors (WZ4003 or HTH-01-015) suppress proliferation by reducing the population of cells in S-phase and mitosis, an effect that can be rescued by overexpression of a NUAK1 mutant in which Ser476 and Ser480 are mutated to alanine. Finally, previous work has suggested that NUAK1 phosphorylates and inhibits PP1βMYPT1 (where PP1 is protein phosphatase 1) and that a major role for the PP1βMYPT1 complex is to inhibit PLK1 by dephosphorylating its T-loop (Thr210). We demonstrate that activation of NUAK1 leads to a striking increase in phosphorylation of PLK1 at Thr210, an effect that is suppressed by NUAK1 inhibitors. Our data link NUAK1 to important cell-cycle signalling components (CDK, PLK and SCFβTrCP) and suggest that NUAK1 plays a role in stimulating S-phase, as well as PLK1 activity via its ability to regulate the PP1βMYPT1 phosphatase.
Platinum nanoparticles prepared by a plasma-chemical reduction method (2005)

Platinum nanoparticles were prepared by reducing H2PtCl6 dissolved in water with the help of hydrogen plasma generated right above the aqueous solution, thus the small surface area of the solution contacting the plasma becomes the active reaction zone. This simple method to fabricate the nanoparticles did not necessitate use of reducing agents and polymer protective materials. The average particle size was around 2 nm with the solution temperature set at 10 °C.
Adaptive image segmentation based on color and texture (2003)

We propose an image segmentation algorithm that is based on spatially adaptive color and texture features. The features are first developed independently, and then combined to obtain an overall segmentation. Texture feature estimation requires a finite neighborhood which limits the spatial resolution of texture segmentation, while color segmentation provides accurate and precise edge localization. We combine a previously proposed adaptive clustering algorithm for color segmentation with a simple but effective texture segmentation approach to obtain an overall image segmentation. Our focus is in the domain of photographic images with an essentially unlimited range of topics. The images are assumed to be of relatively low resolution and may be degraded or compressed.
NMR Characterization of Immunoglobulin G Fc Glycan Motion on Enzymatic Sialylation (2012)

The terminal carbohydrate residues of the N-glycan on the immunoglobulin G (IgG) fragment crystallizable (Fc) determine whether IgG activates pro- or anti-inflammatory receptors. The IgG Fc alone becomes potently anti-inflammatory upon addition of α2-6-linked N-acetylneuraminic acid residues to the N-glycan, stimulating interest in use of this entity in novel therapies for autoimmune disease [Kaneko et al. (2006) Science313, 670-3]. Complete Fc sialylation has, however, been deemed challenging due to a combination of branch specificity and perceived protection by glycan-protein interactions. Here we report the preparation of high levels of disialylated Fc by using sufficient amounts of a highly active α2-6 sialyltransferase (ST6Gal1) preparation expressed in a transiently transformed human cell culture. Surprisingly, ST6Gal1 sialylated the two termini of the complex-type binantennary glycan in a manner remarkably similar to that observed for the free N-glycan, suggesting the Fc polypeptide does not greatly influence ST6Gal1 specificity. In addition, sialylation of either branch terminus does not appear to dramatically alter the motional behavior of the N-glycan as judged by solution NMR spectroscopy. Together these, data suggest the N-glycan occupies two distinct states: one with both glycan termini sequestered from enzymatic modification by an α1-6Man-branch interaction with the polypeptide surface and the other with both glycan termini exposed to the bulk solvent and free from glycan-polypeptide interactions. The results suggest new modes by which disialylated Fc can act as an anti-inflammatory effector.
Analysis of factor VIII coagulant antigen in normal, thrombin-treated, and hemophilic plasma. (1981)

The relationship between Factor VIII coagulant antigen (VIII:CAg) and Factor VIII-associated von Willebrand factor (VIII:vWF), and the effect of thrombin on VIII:CAg have been determined in plasma by using complexes of VIII:CAg and 125I-labeled human anti-VIII:CAg-Fab. Antibody-treated plasma samples were electrophoresed on NaDodSO4/polyacrylamide agarose gels and analyzed by autoradiography. The major VIII:CAg-125I-labeled Fab complex that persisted in NaDodSO4 had Mr 3.2 x 10(5). This Mr value was confirmed by column chromatography and sucrose density centrifugation and is presumed to reflect a free VIII:CAg of Mr 2.7 x 10(5). Minor bands were also present on autoradiograms of normal plasma corresponding to Mr values of 2.5, 1.85, and 1.7 x 10(5) (free VIII:CAg related proteins with Mr values of 2.0, 1.35, and 1.2 x 10(5), respectively). None of the VIII:CAg bands was present in plasma samples from five patients with severe hemophilia A. No radioactivity was associated with VIII:vWF multimers on NaDodSO4 gels. Thrombin treatment of normal plasma eliminated the radioactive band at 3.2 x 10(5) and increased the intensity of a band of Mr 1.7 x 10(5). Generation of this presumed VIII:CAg fragment of Mr is approximately equal to 1.2 x 10(5) coincided with a thrombin-induced increase in Factor VIII coagulant activity. These data demonstrate that the form of VIII:CAg detected in normal plasma is not covalently linked to VIII:vWF multimers and is absent in plasma from five hemophilia A patients. Thrombin-induced proteolysis of VIII:CAg can be detected in microliter quantities of normal plasma.
Facile Synthesis of Single Crystal Vanadium Disulfide Nanosheets by Chemical Vapor Deposition for Efficient Hydrogen Evolution Reaction (2015)

A facile chemical vapor deposition method to prepare single-crystalline VS2 nanosheets for the hydrogen evolution reaction is reported. The electrocatalytic hydrogen evolution reaction (HER) activities of VS2 show an extremely low overpotential of -68 mV at 10 mA cm(-2), small Tafel slopes of ≈34 mV decade(-1), as well as high stability, demonstrating its potential as a candidate non-noble-metal catalyst for the HER.
Identification of the pleiotropic sacQ gene of Bacillus subtilis (1986)

The sacQ gene of Bacillus subtilis, a pleiotropic gene affecting the expression of a number of secreted gene products, has been identified as a small 46-amino-acid polypeptide. The increased expression of this polypeptide in strains carrying the sacQ36 allele, or in strains carrying the sacQ gene on a high copy plasmid, appears to be responsible for the phenotype of higher levels of proteases seen in these strains. A deletion of the sacQ gene had no apparent phenotype, indicating that it is not an essential gene.
Internet of Things for Special Materials Transportation Vehicles (2013)

Special materials (weapons, dangerous goods, valuables, financial, etc.) lack of safety supervision in the transport and distribution process, blind spot in the transportation process, we propose a transport vehicle monitoring system based on Internet of Things technology designed to solve the above business needs. The system uses a transport fleet Command System by wireless ad hoc network, to provide effective protection for the transport fleet, comprehensive utilization of things sensing technology to master the comprehensive information of the transport vehicles and supplies for the safe transport of the materials escort, transport vehicle as a mobile warehouse, the use of RFID and bar code technology to build mobile warehouse management system, a comprehensive collection the entire warehouse business aspects of the operation data, to the meticulous management of the target has taken firm steps.