gebhart/patrepeval huggingface dataset:
Role of the inositol phosphatase SHIP in B cell receptor-induced Ca2+ oscillatory response. (1998)

Src homology-2 domain-containing inositol polyphosphate 5'-phosphatase (SHIP) is a recently identified protein that has been implicated as an important signaling molecule. Although SHIP has been shown to participate in the FcgammaRIIB-mediated inhibitory signal, the functional role of SHIP in activation responses by immunoreceptor tyrosine-based activation motif-bearing receptors such as B cell receptor (BCR) remains unclear. Indeed, it has been proposed that SHIP serves as a linking molecule for the regulation of the extracellular signal-regulated kinase pathway in BCR signaling, because SHIP associates with Shc. We now report that SHIP-deficient DT40 B cells display enhanced Ca2+ mobilization in response to BCR ligation, whereas extracellular signal-regulated kinase activation is unaffected. This Ca2+ enhancement is due to a sustained intracellular Ca2+ increase or to long-lasting Ca2+ oscillations by loss of SHIP, as revealed by single-cell Ca2+ imaging analysis. These results demonstrate the importance of SHIP in B cell activation by the modulation of Ca2+ mobilization.
Exchange in vitro of Subunits between Enzymes from Different Organisms: Chimeras of Enzymes (1976)

Abstract Most intracellular enzymes are made up of several identical or different subunits. The more remote any two organisms are phylogenetically, the greater will be the differences in amino acid sequence of a given enzyme. Nevertheless, numerous examples exist of specific association between chemically different subunits, or even of the formation of enzyme chimeras. They span not only the boundaries between related organisms but also the deep rift between prokaryotic and eukaryotic cells. Exchange of subunits between enzymes of similar activity but differing origin can be rationalized by assuming enzymes to posses functionally defined types of three‐dimensional structures.
A novel L-ascorbic acid and peptide conjugate with increased stability and collagen biosynthesis (2009)

L-ascorbic acid (Vitamin C) and peptide are both useful compounds for collagen biosynthesis in cosmeceuticals (cosmetic and pharmaceutical fields). The instability of these compounds, however, limit their application in these industries. In this report, we describe the development of a novel compound, Stabilized Ascorbyl Pentapeptide (SAP), which physically is much more stable than L-ascorbic acid in water. The inhibitory effects of this SAP compound on tyrosinase and melanin synthesis is comparable to that of L-ascorbic acid. Importantly, the SAP compound displays no cytotoxicity at a high concentration (5 mM). The ability of SAP to promote collagen biosynthesis is greater than that of L-ascorbic acid or the KTTKS peptide alone. Considering the in vitro stability and functional effects, our data strongly suggest that the SAP compound is a good candidate not only as a cosmetic ingredient, but also as a wound healing agent.
Self‐Assembly of Multi‐nanozymes to Mimic an Intracellular Antioxidant Defense System (2016)

In this work, for the first time, we constructed a novel multi-nanozymes cooperative platform to mimic intracellular antioxidant enzyme-based defense system. V2 O5 nanowire served as a glutathione peroxidase (GPx) mimic while MnO2 nanoparticle was used to mimic superoxide dismutase (SOD) and catalase (CAT). Dopamine was used as a linker to achieve the assembling of the nanomaterials. The obtained V2 O5 @pDA@MnO2 nanocomposite could serve as one multi-nanozyme model to mimic intracellular antioxidant enzyme-based defense procedure in which, for example SOD, CAT, and GPx co-participate. In addition, through assembling with dopamine, the hybrid nanocomposites provided synergistic antioxidative effect. Importantly, both in vitro and in vivo experiments demonstrated that our biocompatible system exhibited excellent intracellular reactive oxygen species (ROS) removal ability to protect cell components against oxidative stress, showing its potential application in inflammation therapy.
Insulin-like growth factor binding protein-3 mediates tumor necrosis factor-alpha-induced apoptosis: role of Bcl-2 phosphorylation. (2002)

The insulin-like growth factor (IGF)-independent effects of insulin-like growth factor binding protein-3 (IGFBP-3) to effect cellular apoptosis have now been described in various cellular systems. IGFBP-3 mediates transforming growth factor-beta-induced apoptosis. Other growth-inhibitory and apoptosis-inducing agents such as tumor necrosis factor-alpha (TNF-alpha) and the tumor suppressor gene p53 also induce IGFBP-3. In this report, we demonstrate the role of IGFBP-3 as a mediator of apoptosis induced by TNF-alpha and elucidate the process involved in its signaling mechanism. Treatment of PC-3 cells with TNF-alpha resulted in the induction of IGFBP-3 expression in a dose- and time-dependent fashion and also induced apoptosis. TNF-alpha-induced apoptosis was prevented by cotreatment with IGFBP-3 neutralizing antibodies or IGFBP-3-specific antisense thiolated oligonucleotides. Both IGFBP-3 and TNF-alpha treatment increased the levels of the inactive, serine phosphorylated form of the survival protein Bcl-2. The effect of TNF-alpha on Bcl-2 serine phosphorylation was blocked by IGFBP-3 antisense oligomers. These findings confirm that IGFBP-3 is essential for TNF-alpha-induced apoptosis in PC-3 cells and that this IGFBP-3 effect includes the inactivation of Bcl-2 through serine phosphorylation.
Relative Effectiveness of Phenylhydrazine Treatment and Hemorrhage in the Production of an Erythropoietic Factor (1956)

Abstract Active plasma extract has been prepared from rabbits made anemic by phenylhydrazine treatment, but in every case where rabbits were made equally anemic by bleeding the only peripheral evidence of erythropoietic activity found after injection of plasma filtrate was the inducement of a moderate reticulocytosis in a heterologous recipient. In rabbits bled on successive days until their hematocrits were equal to paired animals made anemic by phenylhydrazine injection, there is, nevertheless, a lower oxygen carrying capacity in the phenylhydrazine injected animals. The latter have methemoglobin concenstrations amounting to approximately 24 per cent of the total blood pigment while in the bled rabbits the methemoglobin level is practically zero. Plasma extracts from phenylhydrazine treated rabbits with apparently normal or very slightly damaged livers produce factor comparable in potency to that obtained from bled animals. The most highly active preparations are obtained in phenylhydrazine treated rabbits which have severely damaged livers. The proposed hypothesis is that the factor is produced in quantity only when the effective hematocrit is below 12 to 15 per cent and when the liver is not capable of rapid active destruction of the factor or is not producing an otherwise normally occurring inhibitor. The factor obtained from phenylhydrazine treated rabbits with liver damage is markedly active as shown by significant increase in blood volume and red cell mass, an elevated red blood cell count and hematocrit, and an intense reticulocytosis.
Preparation of Diphenylacetonitrile (1948)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of DiphenylacetonitrileWm. Bradley Reid Jr. and James H. HunterCite this: J. Am. Chem. Soc. 1948, 70, 10, 3515Publication Date (Print):October 1, 1948Publication History Published online1 May 2002Published inissue 1 October 1948https://pubs.acs.org/doi/10.1021/ja01190a509https://doi.org/10.1021/ja01190a509research-articleACS PublicationsRequest reuse permissionsArticle Views188Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Luminescent Blinking from Silver Nanostructures (2003)

Silver nanostructures deposited on glass showed luminescent blinking when excited at a high 442 nm irradiance. The irradiance required to photoactivate the silver, was dependent on the nature of the silver nanostructures. Silver fractal-like structures were found to be highly emissive, requiring only ≈30 W/cm(2) for photoactivation as compared to silver island films and spin-coated silver colloids, which required a significantly higher irradiance, > 100 W/cm(2), to observe similar luminescent emission. In contrast to our recent findings for gold colloids, foci with different color blinking were also observed, with an increase in luminescence intensity as a function of time. We place these findings in context with recent work from our laboratory which employs these silver nanostructures for applications in metal-enhanced fluorescence, a relatively new phenomenon in fluorescence, whereby metallic particles, colloids, and fractal-like structures can modify the intrinsic radiative decay rate of close proximity fluorescent species. These effects are a consequence of localized changes in photonic mode density around the fluorophores, and we can now report are typically observed at significantly lower illumination intensities as compared to those required to photoactivate silver. Subsequently, our findings strongly suggest that the enhanced fluorescence emission of fluorophores positioned in close proximity to metallic silver structures is not due to either intrinsic silver blinking, or indeed the silver luminescence pumping the fluorophore. Further, the intrinsic luminescence properties of silver reported here, suggest a new class of luminescence probes and labels.
The evolving role of lyophilized plasma in remote damage control resuscitation in the French Armed Forces Health Service (2013)

Freeze-dried plasma was developed by the US Army for the resuscitation of combat casualties during World War II. The French Military Blood Institute began producing French lyophilized plasma (FLYP) in 1949, in accordance with French blood product guidelines. Since 2010, a photochemical pathogen inactivation process has been implemented to reduce the remaining transfusion-related infectious risk. All quality controls for this procedure verify that the hemostatic properties of FLYP are conserved. FLYP is compatible with all blood types, can be stored at room temperature for 2 years, and its reconstitution requires less than 6 minutes. As a result, FLYP allows quick delivery of all the coagulation proteins and the application of a 1:1 ratio of FLYP and red blood cells in the context of a massive transfusion. Hemovigilance data collected in France since 1994 have included FLYP. Results indicate no reporting of infection related to the use of FLYP. Clinical monitoring with a focus on hemostasis was implemented in 2002 and expanded in 2010. The data, obtained from overseas operations, confirmed the indications, the safety and the clinical efficacy of FLYP. Further research is needed to determine specific indications for FLYP in the therapeutic management of civilian patients with severe hemorrhage.
Combination antiviral therapy controls severe post‐liver transplant recurrence of hepatitis B virus infection (1993)

The authors have successfully used combination ganciclovir and foscarnet chemotherapy to control viral replication following liver transplantation in a patient with severe recurrence of hepatitis B virus (HBV) infection. The disease was characterized by extremely high viraemias, deteriorating liver function, and high levels of intra-hepatic hepatitis B core antigen (HBcAg) and hepatitis B surface antigen (HBsAg). Treatment resulted in a greater than 30-fold reduction in serum HBV DNA and HBsAg levels. Liver function tests returned to normal and the histological progression of the disease was arrested. Hepatic cytoplasmic HBsAg decreased substantially but there was little change in HBcAg, implicating HBsAg rather than HBcAg in the liver injury. Combination antiviral chemotherapy using agents such as ganciclovir and foscarnet may offer a new approach to the management of post-transplant recurrence of HBV.
Understanding Visual Concepts with Continuation Learning (2016)

We introduce a neural network architecture and a learning algorithm to produce factorized symbolic representations. We propose to learn these concepts by observing consecutive frames, letting all the components of the hidden representation except a small discrete set (gating units) be predicted from the previous frame, and let the factors of variation in the next frame be represented entirely by these discrete gated units (corresponding to symbolic representations). We demonstrate the efficacy of our approach on datasets of faces undergoing 3D transformations and Atari 2600 games.
Ferroelectricity of HfZrO<sub>2</sub> in Energy Landscape With Surface Potential Gain for Low-Power Steep-Slope Transistors (2015)

The corresponding energy landscape and surface potential are deduced from the experimental ferroelectricity of HfZrO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> (HZO) for low-power steep-slope transistor applications. The anti-ferroelectric (AFE) in annealed 600°C HZO extracted electrostatic potential gain from the measured polarization hysteresis loop and calculated subthreshold swing 33 mV/dec over six decades of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$I_{\rm DS} $ </tex-math></inline-formula> . A feasible concept of coupling the AFE HZO is experimentally established with the validity of negative capacitance and beneficial for steep-slope FET development in future generation.
Imaging with Mass Spectrometry (1999)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImaging with Mass SpectrometryM. L. Pacholski and N. WinogradView Author Information Department of Chemistry, Penn State University, 184 Materials Research Institute Building, University Park, Pennsylvania 16802 Cite this: Chem. Rev. 1999, 99, 10, 2977–3006Publication Date (Web):September 15, 1999Publication History Received16 February 1999Revised18 May 1999Published online15 September 1999Published inissue 13 October 1999https://pubs.acs.org/doi/10.1021/cr980137whttps://doi.org/10.1021/cr980137wresearch-articleACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views2369Altmetric-Citations266LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Grain boundaries,Imaging,Ions,Layers,Oxides Get e-Alerts
Two Mhc class I and two Mhc class II genes map to the chicken Rfp-Y system outside the B complex. (1994)

Gene sequences highly similar to major histocompatibility complex (Mhc) class I and class II genes were recently recognized as mapping to a site in the genome of the chicken separate from the Mhc class I, class II, and B-G genes of the major histocompatibility (B) complex. The present study was undertaken to see whether this complex of Mhc-like genes designated as restriction fragment pattern Y (Rfp-Y) might reside in one of three clusters of cosmid clones contained within the molecular map of chicken Mhc genes, since only two of the three clusters can be assigned to the B system. To determine whether the third cluster (cluster II/IV) might contain Rfp-Y, a subclone (18.1) from within cluster II/IV near a polymorphic lectin gene was used to analyze the DNA of families in which Rfp-Y haplotypes are known to be segregating. The restriction fragment polymorphisms revealed by the 18.1 probe were found to segregate in parallel with the restriction fragment polymorphisms defining the Rfp-Y haplotypes, thus establishing the location of Rfp-Y within cosmid cluster II/IV. Two of six Mhc class I genes and two of five Mhc class II genes map to cosmid cluster II/IV, so a substantial fraction of chicken Mhc genes, including at least one that may be expressed, are located in a chromosomal region separate from the B system. In further linkage analyses, Rfp-Y was found to assort independently from more than 400 markers in the present linkage map of the chicken genome.
Parallax Panoramagrams Made with a Large Diameter Lens (1930)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Herbert E. Ives, "Parallax Panoramagrams Made with a Large Diameter Lens," J. Opt. Soc. Am. 20, 332-342 (1930) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Changes in Adeno-Associated Virus-Mediated Gene Delivery in Retinal Degeneration (2009)

Gene therapies for retinal degeneration have relied on subretinal delivery of viral vectors carrying therapeutic DNA. The subretinal injection is clearly not ideal as it limits the viral transduction profile to a focal region at the injection site and negatively affects the neural retina by detaching it from the supportive retinal pigment epithelium (RPE). We assessed changes in adeno-associated virus (AAV) dispersion and transduction in the degenerating rat retina after intravitreal delivery. We observed a significant increase in AAV-mediated gene transfer in the diseased compared with normal retina, the extent of which depends on the AAV serotype injected. We also identified key structural changes that correspond to increased viral infectivity. Particle diffusion and transgene accumulation in normal and diseased retina were monitored via fluorescent labeling of viral capsids and quantitative PCR. Viral particles were observed to accumulate at the vitreoretinal junction in normal retina, whereas particles spread into the outer retina and RPE in degenerated tissue. Immunohistochemistry illustrates remarkable changes in the architecture of the inner limiting membrane, which are likely to underlie the increased viral transduction in diseased retina. These data highlight the importance of characterizing gene delivery vectors in diseased tissue as structural and biochemical changes can alter viral vector transduction patterns. Furthermore, these results indicate that gene delivery to the outer nuclear layer may be achieved by noninvasive intravitreal AAV administration in the diseased state.
Optimization of MUSIC algorithm for angle of arrival estimation in wireless communications (2013)

Smart Antennas are phased array antennas with smart signal processing algorithms used to identify the angle of arrival (AOA) of the signal, which can be used subsequently to calculate beam-forming vectors needed to track and locate the intended mobile set. This concept is called space division multiple access (SDMA) which enables a higher capacity and data rates for all modern wireless communications by focusing the antenna beam on the intended user. This enables wide coverage and very low interference and also adding new applications like location based services. MUltiple SIgnal Classification (MUSIC) is a well-known high resolution eigen structure method, extensively used to estimate the number of signals, and their angles of arrival. In this paper we investigate the possibility of optimization of some key parameters of the MUSIC algorithm that can enhance the performance of the estimation process. This leads to an increased accuracy in determining the directions of multiple users and beam-forming (Gross, 2005).
3G Radio Access Evolution-HSPA and LTE for Mobile Broadband- (2009)

This paper provides an overview of the 3GPP radio-access technologies for mobile broadband — HSPA and its evolution, and LTE. The paper also discusses the current stage of the 3GPP activities on evolving LTE towards LTE-Advanced and full IMT-Advanced compliance.
Multiple Domains in a Ribozyme Construct Confer Increased Suppressive Activity in Monkey Cells (1994)

An expression vector designed to express a long (approximately 1 kb) RNA containing multiple ribozyme domains was cotransfected into mammalian cells with a plasmid encoding, as target, messenger RNA for chloramphenicol acetyltransferase (CAT). In comparative studies the multimeric ribozyme construct proved to be significantly more effective at suppressing CAT expression than either the corresponding antisense RNA, or a transcript carrying a single ribozyme domain. Suppression of gene activity was apparently specific because expression of an independently expressed gene for human growth hormone was unaffected. The profile of CAT RNA extracted from transfected cells was consistent with RNA cleavage.
Correction: Corrigendum: Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming (2017)

This corrects the article DOI: 10.1038/ncomms11208.
PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALIS (2002)

Pre-inoculating urinary catheters with E. coli 83972 significantly impedes catheter colonization by Enterococcus: These promising in vitro results prompt the clinical investigation of this particular application of bacterial interference.
Synthesis and characterization of one-dimensional K0.27MnO2 center dot 0.5H(2)O (2005)

Potassium manganese oxide (K(0.27)MnO(2)center dot 0.5H(2)O) nanowires/ribbons have been synthesized successfully by a simple hydrothermal method at low temperature. The nanoribbons have rectangular sections with widths of 50-200 nm, thicknesses of 20-25nm, and lengths up to hundreds of micrometers; nanowires have diameters of 20-30nm and lengths up to hundreds of micrometers. A plausible formation mechanism of the one-dimensional nanostructure and the effect of the anion effect used for the starting reactants on the product are discussed. Temperature dependence resistivity of one nanowire follows the small polaron theory p = AT exp(E-a/k(B)T), and a small magnetoresistance was observed in the paramagnetic potassium-intercalated manganese oxides. (c) 2005 Elsevier B.V. All rights reserved.