gebhart/patrepeval huggingface dataset:
Antiviral and anticancer ribozymes (1994)

Molecular biologists are beginning to apply the site-specific RNase properties of ribozymes to gene therapy for HIV infection and cancer. Requirements for substrate recognition and cleavage are being finely mapped. Methods of augmenting intracellular cleavage and of dissecting observed discrepancies between in vitro and cellular activity are being explored. Antiviral efficacy against HIV type 1 has been seen in tissue culture for both hammerhead and hairpin ribozymes, and a phase I clinical trial for ex vivo T-cell gene therapy is planned. Oncogene transcripts, such as the bcr/abl fusion messenger RNA and ras, have also been targeted with ribozymes.
Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. (1982)

A new candidate hormone--designated peptide YY (PYY)--has been isolated from extracts of porcine upper intestinal tissue by using a novel chemical assay method. This peptide has been detected in the extracts by the presence of its unusual COOH-terminal tyrosine amide structure. The peptide consists of a linear chain of 36 amino acids and its complete amino acid sequence is: Tyr-Pro-Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr -Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2. This peptide has a distinct sequence homology to the pancreatic polypeptide. It is therefore proposed that PYY and the pancreatic polypeptide together form a new peptide family. PYY has been found to strongly inhibit both secretin- and cholecystokinin-stimulated pancreatic secretion in the anesthetized cat.
Characterization and expression of the human alpha beta T cell receptor by using a framework monoclonal antibody. (1987)

A monoclonal antibody (mAb) with framework reactivity against the T cell receptor (TCR) alpha beta complex is characterized. The mAb, beta Framework 1 (beta F1) is capable of immunoprecipitating the TCR alpha beta complex from 125I-labeled human T cell tumors, immunocompetent T cell clones, and peripheral blood lymphocytes (PBL). beta F1 recognizes the separated TCR beta subunit in Western blotting. Because it does not bind to the surface of viable T cells but does react with the plasma membrane form of the TCR after treatment with membrane solubilizing agents, the beta F1 mAb reacts with a "hidden" determinant on the TCR beta subunit. After solubilization with 70% ethanol, the TCR alpha beta complex is shown to exist on greater than 92% of T3+ human PBL, whereas 2 to 8% of T3+ PBL do not react with the mAb. The beta F1 mAb demonstrates the existence of differently glycosylated surface 125I-labeled TCR alpha-chains (alpha, alpha', alpha") in association with a common TCR beta-chain on the HPB-MLT T cell leukemia. Reactivity of the beta F1 mAb on thymus tissue sections is similar to that of anti-Leu-4 (anti-T3). The beta F1 mAb should prove useful as a research tool for both the immunochemical characterization and isolation of virtually any alpha beta T cell receptor, whether from individual T cell clones or polyclonal populations of T lymphocytes. Recognition of T cell receptors in histologic tissue sections suggests that the beta F1 mAb may be useful in the clinical diagnosis of T cell lineage neoplasms. In failing to recognize all T3+ lymphocytes, it allows the identification of novel populations of T3+ lymphocytes that may express non-alpha, non-beta T cell receptors.
Effects of Glucosamine and Chondroitin Sulfate on Cartilage Metabolism in OA: Outlook on Other Nutrient Partners Especially Omega-3 Fatty Acids (2011)

Osteoarthritis (OA) is a degenerative joint disease that is characterized by increasing loss of cartilage, remodeling of the periarticular bone, and inflammation of the synovial membrane. Besides the common OA therapy with nonsteroidal anti-inflammatory drugs (NSAIDs), the treatment with chondroprotectives, such as glucosamine sulfate, chondroitin sulfate, hyaluronic acid, collagen hydrolysate, or nutrients, such as antioxidants and omega-3 fatty acids is a promising therapeutic approach. Numerous clinical studies have demonstrated that the targeted administration of selected micronutrients leads to a more effective reduction of OA symptoms, with less adverse events. Their chondroprotective action can be explained by a dual mechanism: (1) as basic components of cartilage and synovial fluid, they stimulate the anabolic process of the cartilage metabolism; (2) their anti-inflammatory action can delay many inflammation-induced catabolic processes in the cartilage. These two mechanisms are able to slow the progression of cartilage destruction and may help to regenerate the joint structure, leading to reduced pain and increased mobility of the affected joint.
Multi-Modulus Blind Equalizations for Coherent Quadrature Duobinary Spectrum Shaped PM-QPSK Digital Signal Processing (2013)

A novel digital signal processing (DSP) scheme for quadrature duobinary (QDB) spectrum shaped polarization multiplexed quadrature phase shift keying (PM-QPSK) based on multi-modulus blind equalizations (MMBE) is proposed and demonstrated with both simulation and experimental results. The key algorithms for this novel digital signal processing scheme include the cascaded multi-modulus algorithm (CMMA) for blind polarization de-multiplexing, multi-modulus QPSK partitioning frequency offset estimation (FOE) and two stage carrier phase recovery (CPR) with maximum likelihood phase estimation. The final signal is detected by maximum-likelihood sequence detection (MLSD) for data BER measurement. It shows that CMMA have better frequency response performance compared with CMA for noise compression. The performances of FOE and CPR are also investigated with different spectrum shaping bandwidth, group size and laser linewidth. Finally, the feasibility of the proposed digital signal processing scheme is demonstrated by the experiment of 112 Gb/s QDB spectrum shaped PM-QPSK signal with a 25 GHz bandwidth waveshaper for Nyquist WDM channels.
Bacteriophages: an underestimated role in human and animal health? (2014)

Metagenomic approaches applied to viruses have highlighted their prevalence in almost all microbial ecosystems investigated. In all ecosystems, notably those associated with humans or animals, the viral fraction is dominated by bacteriophages. Whether they contribute to dysbiosis, i.e., the departure from microbiota composition in symbiosis at equilibrium and entry into a state favoring human or animal disease is unknown at present. This review summarizes what has been learnt on phages associated with human and animal microbiota, and focuses on examples illustrating the several ways by which phages may contribute to a shift to pathogenesis, either by modifying population equilibrium, by horizontal transfer, or by modulating immunity.
Enzymic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates (1980)

In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction components with respect to their influence on the size of DNA transcripts rather than the incorporation of radio-labeled deoxynucleoside triphosphates into acid-insoluble DNA product. The most dramatic effect on uninterrupted reverse transcription is the presence of physiological concentrations (i.e., 148 mM) of monovalent cation in the reaction mixture, although all of the components of the reaction influence the size of the DNA transcripts synthesized to some extent. The enzymatic conditions described herein for the uninterrupted reverse transcription of large RNA templates (greater than 1000--2000 nucleotides) are superior to those described previously because they are reproducible, do not require the presence of ribonuclease inhibitors, and do not result in the precipitation of components of the reaction mixture during incubation.
Degradation and bound residue formation of atrazine in a Western Tennessee soil (1991)

Abstract Biological and physical degradation, and metabolite and soil-bound residue formation, of [U-ring-14C]atrazine (2-chloro-4-[ethylamino]-6-[isopropylamino]-1,3,5-triazine) were characterized in a western Tennessee soil using laboratory and field assays. Biological mineralization of [14C]atrazine was examined in the laboratory using intact surface soil cores in sealed microcosms. Physical mineralization was determined using gamma-radiation-sterilized soil microcosms. Metabolites measured were deethylatrazine (DEA), deisopropylatrazine (DIA), dealkylatrazine (DAA) and hydroxyatrazine (HYA). Atrazine was applied at a rate of 2.2 kg/ha (˜ 5.6 μg/g soil) to soil microcosms in two studies and 3.2 kg/ha to the field. Evolution of 14CO2 from [14C]atrazine in nonirradiated soil microcosms increased to 12 and 28% of the radiolabel added after 180 d incubation. Irradiated soil microcosms evolved less than 0.07% of the radiolabel as 14CO2 for both studies after the same period. The concentrations of metabolites throughout the laboratory and field studies were: HYA &amp;gt; DEA &amp;gt; DIA &amp;gt; DAA. The highest concentration of HYA detected during microcosm and field studies was approximately 0.5 μg/g soil. Atrazine dissipation rates and metabolite production in nonirradiated microcosms were comparable to field results. Atrazine concentrations after 180 d in nonirradiated microcosm and field samples were lower than HYA, and either equivalent to or greater than DEA. The DAA was not detected throughout microcosm or field studies. Rates of dissipation for atrazine followed first-order kinetics. Atrazine half-life, based on extractable residues, was approximately 21 d in the microcosm studies, compared to 14 d in surface field soil. Extractable 14C residues sampled from nonirradiated microcosms decreased in concentration during the incubation period. Soil-bound 14C residues increased and, at the termination of the two studies, accounted for 39 and 43% of the radioactivity added to nonirradiated microcosms. Bound 14C residues in irradiated microcosms accounted for 49 and 48% of the activity after 180 d. Similarity of results between microcosm and field studies suggests that those microcosms may be useful for predicting the fate of atrazine in surface soil.
Varied Effects of Thoracic Irradiation on Peripheral Lymphocyte Subsets in Lung Cancer Patients. (1995)

To investigate the influence of thoracic irradiation on immunological competence in patients with lung cancer, we examined the changes in peripheral blood lymphocyte subsets in 15 patients before and after radiation therapy by two-color flow cytometry techniques. After radiation therapy, the percentage and the absolute number of CD4+CD45RA+ cells (naive T cells) and CD56+ and/or CD16+ cells (NK cells) decreased. The percentage of CD4+human leukocyte antigen-DR(HLA-DR)+ cells (activated CD4T cells) and CD8+HLA-DR+ cells (activated CD8T cells) increased, although the absolute number did not change significantly. Naive T cells may be more selectively damaged than memory T cells by thoracic irradiation, through their recirculation behavior. The reduction of natural killer (NK) cells is disadvantageous for anti-tumor immunity. The percentage of HLA-DR positive T lymphocytes was significantly increased, and thus the possibility of HLA-DR enhancement by irradiation cannot be excluded. Therefore, thoracic irradiation has numerous varied effects on the immunological system of lung cancer patients.
Guideline for the use of antenatal corticosteroids for fetal maturation (2008)

The aim is to present a document, which is based on current evidence and serves as a guideline for use in clinical practice. The following questions are addressed: Is the use of antenatal corticosteroids (ACS) an effective therapy? Who are the candidates for antenatal corticosteroid therapy? Is there benefit after 34 weeks' gestation? When is the optimal time to treat? Which are the optimal steroids; what is the ideal dose and route of administration? Are there any contraindications to the administration of ACS? Are antenatal corticosteroids indicated in women with premature rupture of membranes (PROM)? Is the use of ACS recommended in pregnancies complicated by maternal diabetes mellitus? Should the treatment with corticosteroids be repeated?
Whisper of Magnetism Tells Molecules Apart (2002)

High-energy physicists aren't the only scientists with a lust for power. For decades, chemists have built ever stronger magnets to improve nuclear magnetic resonance (NMR) spectroscopy, a technique that gleans the structure of molecules from the unique magnetic signatures of their component atoms. But generating those high magnetic fields is expensive, which drives up the cost of those probes and related medical imaging scanners.
Evaluating voltage islands in CMPs under process variations (2007)

Parameter variations are a major factor causing power-performance asymmetry in chip multiprocessors. In this paper, we analyze the effects of with-in-die (WID) process variations on chip multicore processors and then apply a variable voltage island scheme to minimize power dissipation. Our idea is based on the observation that due to process variations, the critical paths in each core are likely to have a different latencies resulting in core-to-core (C2C) variations. As a result, each core can operate correctly under different supply voltage levels, achieving an optimal power consumption level. Particularly, we analyze voltage islands at different granularities ranging from a single core to a group of cores. We show that the dynamic power consumption can be reduced by up to 36.2% when each core can set its individual supply voltage level. In addition, for most manufacturing technologies, significant power savings can be achieved with only a few voltage islands on the whole chip: a single customized voltage setting can reduce the power consumption by up to 31.5%. Since the nominal operating frequency remains unchanged after the modifications, our scheme incurs no performance overhead.
Information Extraction from Web-scale N-gram Data (2010)

Search engines are increasingly relying on structured data to provide direct answers to certain types of queries. However, extracting such structured data from text is challenging, especially due to the scarcity of explicitly expressed knowledge. Even when relying on large document collections, pattern-based information extraction approaches typically expose only insufficient amounts of information. This paper evaluates to what extent n-gram statistics, derived from volumes of texts several orders of magnitude larger than typical corpora, can allow us to overcome this bottleneck. An extensive experimental evaluation is provided for three different binary relations, comparing different sources of n-gram data as well as different learning algorithms.
Synthetic approach to pentaleno[2,1-b:5,4-b']diindoles (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic approach to pentaleno[2,1-b:5,4-b']diindolesKotha Sambasivarao, Sean Hollinshead, Desiree Grubisha, Frank Laib, Dennis Bennett, and James M. CookCite this: J. Org. Chem. 1990, 55, 12, 3858–3866Publication Date (Print):June 1, 1990Publication History Published online1 May 2002Published inissue 1 June 1990https://pubs.acs.org/doi/10.1021/jo00299a031https://doi.org/10.1021/jo00299a031research-articleACS PublicationsRequest reuse permissionsArticle Views370Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Monoclonal and bispecific antibodies as novel therapeutics (2006)

Gene amplification, over-expression, and mutation of growth factors, or the receptors themselves, causes increased signaling through receptor kinases, which has been implicated in many human cancers and is associated with poor prognosis. Tumor growth has been shown to be decreased by interrupting this process of extensive growth factor-mediated signaling by directly targeting either the surface receptor or the ligand and thereby preventing cell survival and promoting apoptosis. Monoclonal antibodies have long been eyed as a potential new class of therapeutics targeting cancer and other diseases. Antibody-based therapy initially entered clinical practice when trastuzumab/Herceptin became the first clinically approved drug against an oncogene product as a well-established blocking reagent for tumors with hyperactivity of epidermal growth factor signaling pathways. In the first part of this review we explain basic terms related to the development of antibody-based drugs, give a brief historic perspective of the field, and also touch on topics such as the "humanization of antibodie" or creation of hybrid antibodies. The second part of the review gives an overview of the clinical usage of bispecific antibodies and antibodies "armed" with cytotoxic agents or enzymes. Further within this section, cancer-specific, site-specific, or signaling pathway-specific therapies are discussed in detail. Among other antibody-based therapeutic products, we discuss: Avastin (bevacizumab), CG76030, Theragyn (pemtumomab), daclizumab (Zenapax), TriAb, MDX-210, Herceptin (trastuzumab), panitumumab (ABX-EGF), mastuzimab (EMD-72000), Erbitux (certuximab, IMC225), Panorex (edrecolomab), STI571, CeaVac, Campath (alemtuizumab), Mylotarg (gemtuzumab, ozogamicin), and many others. The end of the review deliberates upon potential problems associated with cancer immunotherapy.
Novel Somatic and Germline Mutations in Cancer Candidate Genes in Glioblastoma, Melanoma, and Pancreatic Carcinoma (2007)

A recent systematic sequence analysis of well-annotated human protein coding genes or consensus coding sequences led to the identification of 189 genes displaying somatic mutations in breast and colorectal cancers. Based on their mutation prevalence, a subset of these genes was identified as cancer candidate (CAN) genes as they could be potentially involved in cancer. We evaluated the mutational profiles of 19 CAN genes in the highly aggressive tumors: glioblastoma, melanoma, and pancreatic carcinoma. Among other changes, we found novel somatic mutations in EPHA3, MLL3, TECTA, FBXW7, and OBSCN, affecting amino acids not previously found to be mutated in human cancers. Interestingly, we also found a germline nucleotide variant of OBSCN that was previously reported as a somatic mutation. Our results identify specific genetic lesions in glioblastoma, melanoma, and pancreatic cancers and indicate that CAN genes and their mutational profiles are tumor specific. Some of the mutated genes, such as the tyrosine kinase EPHA3, are clearly amenable to pharmacologic intervention and could represent novel therapeutic targets for these incurable cancers. We also speculate that similar to other oncogenes and tumor suppressor genes, mutations affecting OBSCN could be involved in cancer predisposition.
Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. (1984)

Fusion of rat immune spleen cells with mouse myeloma cells resulted in the formation of a stable hybridoma that secretes monoclonal antibody (MAb) directed against murine gamma interferon ( MuIFN -gamma). This MAb specifically neutralized the antiviral activity of a variety of MuIFN -gamma preparations, including a sample produced by recombinant DNA technologies. In contrast, the antiviral activities of a mixture of MuIFN -alpha plus MuIFN -beta, as well as those of rat or human IFN-gamma, were not neutralized by this antibody. The ability of the MAb to inhibit lymphokine-induced macrophage activation was also tested. It was found that in relation to the quantity of antibody needed to completely neutralize antiviral activity, much higher concentrations of MAb were required to abolish the capacity of lymphokine preparations to induce macrophage tumoricidal activity in vitro. The MAb was also coupled to cyanogen bromide-activated Sepharose beads and used as an immunoadsorbent. By reacting lymphokines with MAb coupled to an insoluble matrix, it was possible to show that this immobilized antibody completely and specifically removed from the lymphokine preparations the ability both to invoke macrophage tumoricidal activity and to mediate antiviral activity.
Synthesis and Cytotoxic Activity of Two Novel 1-Dodecylthio-2-decyloxypropyl-3-phosphatidic Acid Conjugates with Gemcitabine and Cytosine Arabinoside (2003)

Cytosine arabinoside (ara-C) and gemcitabine (dFdC) are two standard chemotherapy drugs used in the treatment of patients with various cancers. To alter the pharmacokinetic and pharmacodynamic properties of these molecules, we conjugated a synthetic phospholipid to both ara-C and dFdC and investigated their chemotherapeutic potential. The dFdC conjugate had greater cytotoxic activity compared with the ara-C conjugate and demonstrated notable cytotoxicity against all human cell lines tested.