gebhart/patrepeval huggingface dataset:
Human Coxsackie-Adenovirus Receptor Is Colocalized With Integrins α v β 3 and α v β 5 on the Cardiomyocyte Sarcolemma and Upregulated in Dilated Cardiomyopathy (2001)

Background The coxsackievirus and adenovirus receptor (CAR) was identified as a common cellular receptor for both viruses, but its biological and pathogenic relevance is uncertain. Knowledge of CAR localization in the human cardiovascular system is limited but important with respect to CAR-dependent viral infections and gene transfer using CAR-dependent viral vectors. Methods and Results Explanted failing hearts from 13 patients (8 with dilated cardiomyopathy [DCM] and 5 with other heart diseases [non-DCM]) and normal donor hearts (n=7) were investigated for the expression levels and subcellular localization of CAR and the adenovirus coreceptors α v β 3 and α v β 5 integrins. CAR immunoreactivity was very low in normal and non-DCM hearts, whereas strong CAR signals occurred at the intercalated discs and sarcolemma in 5 of the 8 DCM hearts (62.5%); these strong signals colocalized with both integrins. In all hearts, CAR was detectable in subendothelial layers of the vessel wall, but not on the luminal endothelial surface, and on interstitial cells. Human CAR (hCAR) expressed in rat cardiomyocytes was targeted to cell-cell contacts, which resembled CAR localization in DCM hearts and resulted in 15-fold increased adenovirus uptake. Conclusions Low hCAR abundance may render normal human myocardium resistant to CAR-dependent viruses, whereas re-expression of hCAR, such as that observed in DCM, may be a key determinant of cardiac susceptibility to viral infections. Asymmetric expression of hCAR in the vessel wall may be an important determinant of adenovirus tropism in humans. hCAR subcellular localization in human myocardium and hCAR targeting to cell-cell contacts in cardiomyocyte cultures suggest that hCAR may play a role in cell-cell contact formation.
Identification of aecA mutations in Bacillus subtilis as nucleotide substitutions in the untranslated leader region of the aspartokinase II operon (1991)

Recent genetic mapping of the aspartokinase II (lysC) operon of Bacillus subtilis [M. Petricek. L. Rutberg & L. Hederstedt (1989) FEMS Microbiology Letters 61, 85-88; N.Y. Chen. J. J. Zhang & H. Paulus (1989) Journal of General Microbiology 135, 2931-2940] has shown its chromosomal location to be close to the aecA locus, the mutation of which leads to highly increased levels of aspartokinase II. In order to examine the relationship between lysC and aecA, we have cloned the control regions of the lysC operon from several independent aecA mutants and determined their nucleotide sequences. The nucleotide sequences of the aecA mutants differed from the wild-type sequence by the substitution of one or two nucleotides at two widely separated sites in the transcribed leader region of the lysC operon. To confirm that the observed nucleotide changes are indeed responsible for the AecA phenotype and not simply the reflection of sequence polymorphisms in different B. subtilis strains, we introduced the same nucleotide substitutions as those observed in the aecA strains into the leader region of the wild-type lysC operon by oligonucleotide-directed mutagenesis. The expression of the mutagenized genes was analysed after transcriptional or translational fusion to lacZ in a single-copy integration vector. The levels of beta-galactosidase were greatly elevated by the nucleotide substitutions, with similar increases observed in transcriptional and translational fusions. The high level of expression of beta-galactosidase in the lysC'-lac'Z strains with nucleotide substitutions corresponding to the aecA mutations was resistant to repression by L-lysine but was completely abolished by the inactivation of the lysC promoter.(ABSTRACT TRUNCATED AT 250 WORDS)
Cancer Targets in the Ras Pathway (2005)

Ras proteins play a direct causal role in human cancer and in other diseases. Mutant H-Ras, N-Ras, and K-Ras occur in varying frequencies in different tumor types, for reasons that are not known. Other members of the Ras superfamily may also contribute to cancer. Mutations also occur in downstream pathways, notably B-Raf, PTEN, and PI 3' kinase: These pathways interact at multiple points, including cyclin D1, and act synergistically. In some cases mutations in Ras and effectors are mutually exclusive; in other cases, they coexist. Drugs blocking elements of the pathway are in different stages of clinical development. One of these, the Raf kinase/VEGF-R2 inhibitor Sorafenib, has already been approved for treatment of renal cancer and is being tested in other indications. However, therapeutic targets in the Ras pathway have not yet been fully validated as bona fide targets.
Energy-accuracy trade-off for continuous mobile device location (2010)

Mobile applications often need location data, to update locally relevant information and adapt the device context. While most smartphones do include a GPS receiver, it's frequent use is restricted due to high battery drain. We design and prototype an adaptive location service for mobile devices, a-Loc, that helps reduce this battery drain. Our design is based on the observation that the required location accuracy varies with location, and hence lower energy and lower accuracy localization methods, such as those based on WiFi and cell-tower triangulation, can sometimes be used. Our method automatically determines the dynamic accuracy requirement for mobile search-based applications. As the user moves, both the accuracy requirements and the location sensor errors change. A-Loc continually tunes the energy expenditure to meet the changing accuracy requirements using the available sensors. A Bayesian estimation framework is used to model user location and sensor errors. Experiments are performed with Android G1 and AT&T Tilt phones, on paths that include outdoor and indoor locations, using war-driving data from Google and Microsoft. The experiments show that a-Loc not only provides significant energy savings, but also improves the accuracy achieved, because it uses multiple sensors.
Times to Detection of Bacteria and Yeasts in BACTEC 9240 Blood Culture Bottles (1999)

A 7-day incubation protocol was instituted with the BACTEC 9240 system for a 1-year period to determine the times to detection of clinically relevant organisms. A total of 23,686 blood and 693 sterile body fluid cultures were received; some cultures were held longer by special request. Of 1,609 likely skin contaminants, 42 were recovered on day 5, 34 on day 6, 16 on day 7, and 5 on day 8. Of 2,803 usual pathogens, 34 were recovered on day 5, 24 on day 6, 15 on day 7 and 1 on day 8. Twenty-one of the latter organisms were considered significant laboratory isolates because they were the first isolates from the respective patients. Chart review showed that 10 of 21 were considered clinically significant, but only 3 (all yeasts) affected the treatment of the patient. Our data show that 4 days of incubation were sufficient to recover all clinically relevant bacteria and 6 days were required to recover all clinically relevant yeasts.
Gene expression profiles of prostate cancer reveal involvement of multiple molecular pathways in the metastatic process (2007)

We hypothesize that these genes have a role in the biology of metastatic disease and that they represent potential therapeutic targets for prostate cancer.
Isolation and Characterization of Monoclonal Antibody Charge Variants by Free Flow Isoelectric Focusing (2016)

Capillary isoelectric focusing (cIEF) is widely used in the biopharmaceutical industry to measure the charge distribution of therapeutic proteins. The implementation of this technology has created a new challenge. Capillary volumes are on the order of hundreds of nanoliters and cannot be scaled up for the preparative collection of charge variants. This makes it difficult to identify the charge variants in a cIEF electropherogram. Therefore, preparative IEF methods are needed to fractionate charge variants for characterization. We used free-flow electrophoresis (FFE) to isolate monoclonal antibody charge variants observed in a cIEF electropherogram. The same antibody was also fractionated using the Rotofor and Offgel instruments for comparison. A strategy for purifying the fractionated charge variants and downstream characterization is described. Acidic and basic variants were identified and related back to the analytical cIEF charge profile. This study establishes free-flow isoelectric focusing as a valuable tool for characterizing therapeutic proteins.
MAGIC—Memristor-Aided Logic (2014)

Memristors are passive components with a varying resistance that depends on the previous voltage applied across the device. While memristors are naturally used as memory, memristors can also be used for other applications, including logic circuits. In this brief, a memristor-only logic family, i.e., memristor-aided logic (MAGIC), is presented. In each MAGIC logic gate, memristors serve as an input with previously stored data, and an additional memristor serves as an output. The topology of a MAGIC nor gate is similar to the structure of a common memristor-based crossbar memory array. A MAGIC nor gate can therefore be placed within memory, providing opportunities for novel non-von Neumann computer architectures. Other MAGIC gates also exist (e.g., and, or, not, and nand) and are described in this brief.
Carl Orff: Musical Humanist (2006)

Beyond the obvious knowledge and skills imparted, education respects and enables students in ways that training does not.Composer Carl Orff articulated a central theme of his approach to education also known as Orff-Schulwerk, or schooling in music through active involvement when he wrote: "Anyone who has worked with children or young people in the spirit of Schulwerk will have discovered that it has a humanizing influence which transcends its musical function."This paper is an exploration of the Schulwerk in terms of its humanizing potential as well as a comparason of the Schulwerk to humanism as articulated by Abraham Maslow and Carl Rogers.
Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis (1991)

The gene encoding a 23 kilodalton protein antigen has been cloned from Mycobacterium tuberculosis by screening of a recombinant DNA library with monoclonal antibodies. The product of the gene has been identified as the superoxide dismutase (SOD) of M. tuberculosis on the basis of sequence comparison and by expression of the recombinant protein in a functionally active form. The derived amino acid sequence of M. tuberculosis SOD reveals a close similarity to manganese-containing SODs from other organisms, in spite of the fact that previous studies using the purified enzyme have identified iron as the preferred metal ion ligand. SOD is present in the extracellular fluid of logarithmic-phase cultures of M. tuberculosis, but the structural gene is not preceded by a signal peptide sequence. Insertion of the M. tuberculosis SOD gene into a novel shuttle vector demonstrated the mycobacteria but is ineffective in Escherichia coli.
PHARMACOLOGIC ENHANCEMENT OF WOUND HEALING (1995)

The field of pharmacologic modulation of soft tissue repair is in its infancy. Although the soluble, cellular, and insoluble mediators that govern repair have not been elucidated, the application of pharmacologic concentrations of purified polypeptide growth factors, cytokines, and matrix molecules has nonetheless resulted in the acceleration of normal repair and the reversal of deficient repair in a wide variety of dermal wound models in animals. However, early clinical results using these factors have been less than encouraging, and their potential roles in the armamentarium of chronic wound therapies remain to be established.
cGMP-Dependent Protein Kinase Expression Restores Contractile Function in Cultured Vascular Smooth Muscle Cells (2002)

Vascular diseases, such as atherosclerosis and restenosis following angioplasty or transplantation, are due to abnormal vascular smooth muscle growth and gene expression. The smooth muscle cells (SMC) in response to injury lose their contractile function, become highly proliferative and synthesize and secrete extracellular matrix proteins. Similar changes in the phenotypic properties of vascular SMC occur during in vitro culture. In this report, we examined whether restoration of the expression of the major receptor protein for nitric oxide (NO) signaling in smooth muscle, the guanosine 3':5' cyclic monophosphate (cGMP)-dependent protein kinase (PKG), reestablished contractile function to cultured rat aortic SMC. Contractile function was monitored using the silicone polymer wrinkle assay used previously to determine contractility in cultured mesangial cells. Noncontractile rat aortic smooth muscle cells transfected with the cDNA encoding the type I isoform of PKG, but not those transfected with empty vector, formed discreet wrinkles on the substratum in response to serum indicative of contraction. Treatment of the PKG-expressing SMC with sodium nitroprusside (SNP), an NO donor, and with cGMP analogs, or with the adenylyl cyclase activator, forskolin, and with adenosine 3':5' cyclic monophosphate (cAMP) analogs reduced wrinkling. The expression of a major PKG substrate protein involved in smooth muscle relaxation, heat shock-related protein-20 (HSP20), was also reestablished in PKG-expressing SMC. Treatment of the PKG-expressing SMC with nitroprusside resulted in phosphorylation of HSP20. Collectively, these results indicate that PKG expression is important to establish contractility to SMC in culture.
Ionic Mechanism of Action Potential Prolongation in Ventricular Myocytes From Dogs With Pacing-Induced Heart Failure (1996)

Membrane current abnormalities have been described in human heart failure. To determine whether similar current changes are observed in a large animal model of heart failure, we studied dogs with pacing-induced cardiomyopathy. Myocytes isolated from the midmyocardium of 13 dogs with heart failure induced by 3 to 4 weeks of rapid ventricular pacing and from 16 nonpaced control dogs did not differ in cell surface area or resting membrane potential. Nevertheless, action potential duration (APD) was significantly prolonged in myocytes isolated from failing ventricles (APD at 90% repolarization, 1097 +/- 73 milliseconds [failing hearts, n = 30] versus 842 +/- 56 milliseconds [control hearts, n = 25]; P < .05), and the prominent repolarizing notch in phase 1 was dramatically attenuated. Basal L-type Ca2+ current and whole-cell Na+ current did not differ in cells from failing and from control hearts, but significant differences in K+ currents were observed. The density of the inward rectifier K+ current (IKl) was reduced in cells from failing hearts at test potentials below -90 mV (at -150 mV, -19.1 +/- 2.2 pA/pF [failing hearts, n = 18] versus -32.2 +/- 5.1 pA/pF [control hearts, n = 15]; P < .05). The small outward current component of IKl was also reduced in cells from failing hearts (at -60 mV, 1.7 +/- 0.2 pA/pF [failing hearts] versus 2.5 +/- 0.2 pA/pF [control hearts]; P < .05). The peak of the Ca(2+)-independent transient outward current (Ito) was dramatically reduced in myocytes isolated from failing hearts compared with nonfailing control hearts (at +80 mV, 7.0 +/- 0.9 pA/pF [failing hearts, n = 20] versus 20.4 +/- 3.2 pA/pF [control hearts, n = 15]; P < .001), while the steady state component was unchanged. There were no significant differences in Ito kinetics or single-channel conductance. A reduction in the number of functional Ito channels was demonstrated by nonstationary fluctuation analysis (0.4 +/- 0.03 channels per square micrometer [failing hearts, n = 5] versus 1.2 +/- 0.1 channels per square micrometer [control hearts, n = 3]; P < .001). Pharmacological reduction of Ito by 4-aminopyridine in control myocytes decreased the notch amplitude and prolonged the APD. Current clamp-release experiments in which current was injected for 8 milliseconds to reproduce the notch sufficed to shorten the APD significantly in cells from failing hearts. These data support the hypothesis that downregulation of Ito in pacing-induced heart failure is at least partially responsible for the action potential prolongation. Because the repolarization abnormalities mimic those in cells isolated from failing human ventricular myocardium, canine pacing-induced cardiomyopathy may provide insights into the development of repolarization abnormalities and the mechanisms of sudden death in patients with heart failure.
Structuring operating system aspects (2001)

article Share on Structuring operating system aspects: using AOP to improve OS structure modularity Authors: Yvonne Coady Univ. of British Columbia, Cancouver, B. C., Canada Univ. of British Columbia, Cancouver, B. C., CanadaView Profile , Gregor Kiczales Univ. of British Columbia, Cancouver, B. C., Canada and Xerox PARC Univ. of British Columbia, Cancouver, B. C., Canada and Xerox PARCView Profile , Mike Feeley Univ. of British Columbia, Cancouver, B. C., Canada Univ. of British Columbia, Cancouver, B. C., CanadaView Profile , Norm Hutchinson Univ. of British Columbia, Cancouver, B. C., Canada Univ. of British Columbia, Cancouver, B. C., CanadaView Profile , Joon Suan Ong Univ. of British Columbia, Cancouver, B. C., Canada Univ. of British Columbia, Cancouver, B. C., CanadaView Profile Authors Info & Claims Communications of the ACMVolume 44Issue 10Oct. 2001pp 79–82https://doi.org/10.1145/383845.383863Published:01 October 2001Publication History 16citation2,969DownloadsMetricsTotal Citations16Total Downloads2,969Last 12 Months7Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
A reliable and efficient method for deleting operational sequences in PACs and BACs (2002)

P1-derived artificial chromosomes (PACs) and bacterial artificial chromosomes (BACs) have become very useful as tools to study gene expression and regulation in cells and in transgenic mice. They carry large fragments of genomic DNA (> or =100 kb) and therefore may contain all of the cis-regulatory elements required for expression of a gene. Because of this, even when inserted randomly in the genome, they can emulate the native environment of a gene resulting in a tightly regulated pattern of expression. Because these large genomic clones often contain DNA sequences which can manipulate chromatin at the local level, they become immune to position effects which affect expression of smaller transgenes, and thus their expression is proportional to copy number. Transgenic mice containing large BACs and PACs have become excellent models to examine the regulation of gene expression. Their usefulness would certainly be increased if easy and efficient methods are developed to manipulate them. We describe herein a method to make deletion mutations reliably and efficiently using a novel modification of the Chi-stimulated homologous recombination method. Specifically, we generated and employed a Lox511 'floxed' CAM resistance marker that first affords selection for homologous recombination in Escherichia coli, and then can be easily deleted leaving only a single Lox511 site as the footprint.
MSMAD: a computationally efficient method for the analysis of noisy array CGH data (2009)

Supplementary data are available at Bioinformatics online.
Boron Containing Compounds as Protease Inhibitors (2012)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTBoron Containing Compounds as Protease InhibitorsReem Smoum*, Abraham Rubinstein*, Valery M. Dembitsky*, and Morris SrebnikView Author Information The School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Faculty of Medicine, P.O. Box 12065, Jerusalem 91120, Israel*E-mail: [email protected] (R.S.); [email protected] (A.R.); [email protected] (V.M.D.).Cite this: Chem. Rev. 2012, 112, 7, 4156–4220Publication Date (Web):April 20, 2012Publication History Received7 September 2007Published online20 April 2012Published inissue 11 July 2012https://pubs.acs.org/doi/10.1021/cr608202mhttps://doi.org/10.1021/cr608202mreview-articleACS PublicationsCopyright © 2012 American Chemical SocietyRequest reuse permissionsArticle Views14142Altmetric-Citations346LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Crystal structure,Inhibition,Inhibitors,Monomers,Peptides and proteins Get e-Alerts
Quantenchemische Untersuchung der Zusammenhänge von PE‐, UV‐ und pKa‐Daten bei aromatischen Phosphinen und Aminen (1977)

Abstract Im Rahmen der PPP‐SCF‐CI‐Methode werden PE‐ und UV‐spektroskopische Daten von p ‐substituierten Phenylphosphinen 1 und Anilinen 2 mit Abweichungen von weniger als 2% berechnet. Mit den dabei gewonnenen Parametersätzen lassen sich für diese Verbindungen π‐Lokalisierungsenergien berechnen, die mit den Flüssigphasen‐p K a ‐Werten sehr gut korrelieren. Dagegen ist die Korrelation der aus den experimentellen PE‐Spektren ermittelten vertikalen Ionisationspotentiale der n‐Orbitale mit den p K a ‐Werten unbefriedigend.
Aberrant Cementum Phenotype Associated With the Hypophosphatemic Hyp Mouse (2009)

Mutations in the phosphate-regulating Phex gene of the Hyp mouse resulted in defective cementum development.
7‐Keto‐5β‐ätiansäure‐Derivate. Über Gallensäuren und verwandte Stoffe, 51. Mitteilung (1958)

Abstract Der Abbau der Seitenkette in Cholsäure bis zur Methylketon‐Stufe wird beschrieben. Ausgehend von 3α, 12α‐Dihydroxy‐7α‐acetoxy‐5β‐pregnan‐20‐on (XX) wurden die Pregnan‐Derivate XXIV, XXV, XXVII, XXVIII, XXXI, XXXIII und XXXIV synthetisiert. Die Herstellung des 12‐Keto‐ätiansäureesters XXII erfolgte aus XXV mit Hilfe der Haloformreaktion. Dieser Ester diente als Ausgangsmaterial für die Synthese von 3α‐Acetoxy‐7‐keto‐ätiansäure‐methylester (XLVIII) und 7‐Keto‐ätiansäure‐methylester (VIII) mit gesicherter Konfiguration. Der letztere wurde auf zwei Wegen erhalten. Sowohl VIII wie XLVIII erwiesen sich als identisch mit den von L ARDON auf anderem Weg hergestellten Präparaten. Im Laufe dieser Synthese wurde beobachtet, dass die Verseifung der 7α‐Acetoxy‐Gruppe in XXIII und XL zu zwei isomeren Hydroxy‐ketoestern führt.