gebhart/patrepeval huggingface dataset:
Apoptosis in parasites and parasite-induced apoptosis in the host immune system: a new approach to parasitic diseases (1999)

Apoptosis, a form of programmed cell death (PCD), has been described as essential for normal organogenesis and tissue development, as well as for the proper function of cell-renewal systems in adult organisms. Apoptosis is also pivotal in the pathogenesis of several different diseases. In this paper we discuss, from two different points of view, the role of apoptosis in parasitic diseases. The description of apoptotic death in three different species of heteroxenic trypanosomatids is reviewed, and considerations on the phylogenesis of apoptosis and on the eventual role of PCD on their mechanism of pathogenesis are made. From a different perspective, an increasing body of evidence is making clear that regulation of host cell apoptosis is an important factor on the definition of a host-pathogen interaction. As an example, the molecular mechanisms by which Trypanosoma cruzi is able to induce apoptosis in immunocompetent cells, in a murine model of Chagas' disease, and the consequences of this phenomenon on the outcome of the experimental disease are discussed.
Real-time optical spectrum analysis based on the time-space duality in chirped fiber gratings (2000)

Based on time-space duality, we deduce a time-domain equivalent to the Fraunhofer (far-field) approximation in the problem of spatial diffraction. We can use this equivalence to carry out a real-time optical spectrum analysis, which is shown to be realizable by using, as the dispersive media, filtering devices based on chirped distributed resonant coupling. In particular, we present the design of linearly chirped fiber gratings (reflection configurations) and linearly chirped intermodal couplers (transmission configurations) to work as real-time spectrum analyzers. The proposed systems are shown to work properly by means of simulation tools. Furthermore, we use joint time-frequency signal representations to get a better understanding of the physical processes that determine the behavior of these systems. In this way, we demonstrate that the propagation of a given signal through a chirped fiber grating (or a chirped intermodal coupler), under the temporal Fraunhofer conditions, translates into a temporal separation of the spectral components of the signal. The results of our study indicate potential important applications based on this effect.
Extraction and integration of data from semi-structured documents into business applications (1997)

Collecting useful information along the information Autobahn is a fun window shopping activity which rapidly becomes frustrating. As the technology offers a broader logical connectivity, enables schemes
Screening of Polymers for EOR in High Temperature, High Salinity and Carbonate Reservoir Conditions (2015)

Abstract Polymer flooding is one of the most attractive chemical EOR techniques for sandstone reservoirs however due to complex geological heterogeneity and harsh reservoir conditions its full potential has not been explored in carbonate reservoirs. The main reason behind this limitation is the inability of conventional EOR polymers like HPAM and Xanthan to withstand these conditions. Candidate polymers must provide the required rheology at minimal polymer concentration, be thermally and mechanically stable, and result in a manageable adsorption on the reservoir formation. A polymer screening study has been conducted on a series of polymers, to identify the most suitable candidate that can tolerate the harsh reservoir conditions. Initially, rheological measurements are conducted on a series of polymers followed by filterability, injectivity, static and dynamic adsorption, mechanical and thermal stability testing as a screening criterion for polymers in EOR operations. Amongst the tested polymers polyacrylamide based co- and ter-polymers showed reasonable temperature stability with low salinity tolerance. Whereas, biopolymer Schizophyllan a polysaccharide showed shear thinning behavior with positive thermal stability and salt tolerance. Long-term thermal stability of biopolymer is also conducted at a temperature of 120 °C and salinity up to 220 g/L under anaerobic conditions for over eight months and no viscosity loss is observed. Biopolymer showed acceptable injectivity on cores of permeability more than 30 mD. Core flood effluents viscosity reached 40% compared to injected sample. In addition the mechanical stability and filterability of biopolymer are also discussed. Static as well as dynamic adsorptions of biopolymer have also been studied. The static adsorption on four natural minerals (Calcite, Dolomite, Kaolin, and Silica) as well as formation rocks is reported. Out of these four minerals, maximum and minimum adsorption is observed on Dolomite and Kaolin respectively. The adsorption of biopolymer decreases with salinity and temperature. Adsorption on carbonate reservoir rocks is found to be low when compared to pure calcite and dolomite minerals. Dynamic adsorption on cores with different permeabilites (3 to 165 mD) is measured to be low in magnitude (47.5 to 0.53 µg/g).
Reassessment of the suction blister model of wound healing: introduction of a new higher pressure device (1999)

This higher pressure suction blister system is an effective model for wound healing. Compared to reported methods, it is a better tolerated, more reliable, and more efficient approach to studying in vivo wound repair in human subjects. It is especially well suited for screening the wound healing potential of new pharmacologic agents.
Crystal structure of interleukin 8: symbiosis of NMR and crystallography. (1991)

The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 A x-ray data to an R factor of 0.187 (0.217 at 1.6 A). A comparison indicates some potential differences between the structure in solution and in the crystalline state. Our analysis also predicts that residues 4 through 9 on the amino terminus and the beta-bend, which includes His-33, may be important for receptor binding.
Direct correlation of cytogenetic findings with cell morphology using in situ hybridization: an analysis of suspicious cells in bone marrow specimens of two patients completing therapy for acute lymphoblastic leukemia (1991)

Bone marrow cells from two pediatric patients completing therapy for acute lymphoblastic leukemia were studied using in situ hybridization with an alpha-satellite DNA probe specific for chromosome 17. Morphologic analysis of the end-therapy specimens from each patient had shown small numbers (7.5%, 8.5%) of cells that were suspicious for residual or recurrent disease. These cells could not be morphologically or immunophenotypically distinguished with certainty from immature lymphoid cells (hematogones), which may be present normally, sometimes in increased numbers, in the bone marrow specimens of children. In situ hybridization with a probe to chromosome 17 was used because the leukemic cells from each patient had originally been shown to have an extra copy of this chromosome. In one patient, in situ studies showed a population of cells (106 of 1,000 cells) with three hybridization signals indicating trisomy 17, and thus residual/recurrent leukemia. In the other patient trisomy 17 could not be detected. Additional hybridizations to previously stained bone marrow aspirate smears permitted a direct correlation of the cytogenetic findings with the suspicious cells on a cell-to-cell basis. The questionable cells were identified, photographed, and then re-examined after hybridization. In one patient, 13 of 18 (72%) of the suspicious cells were found to have trisomy 17, whereas in the other patient 0 of 24 (0%) demonstrated an extra copy of this chromosome. These cases illustrate a clinical application of interphase cytogenetic analysis and demonstrate how this technology can be used for direct correlation of cytogenetic findings with cell morphology. This technique should prove useful for the detection of minimal residual disease and for lineage studies in leukemia and myelodysplasia.
Expression of the secretory hepatitis B virus middle surface protein in Pichia pastoris (2002)

The methylotrophic yeast Pichia pastoris has been developed as production systems for recombinant proteins recently. The favourable and most advantageous characteristics of the species have resulted in an increasing number of biotechnological applications. Gene of hepatitis B virus middle surface protein (M) was placed under the control of AOX1 promoter in an integrative Pichia expression vector carrying eight copies of the expression cassette. Through electroporation transformation of SMD1168 and G418 selection, a strain of Pichia pastoris capable of secreting M protein into the medium at a level up to 50 mg/L was constructed. After initially purified, the characterization of the expression products demonstrated that the recombinant protein could be assembled into particles which presented preS2 and S antigenicity and a certain extent of glycosylation. These results have provided a good foundation for further research and development of new hepatitis B virus vaccine.
Frizzled 9 knock-out mice have abnormal B-cell development (2004)

The binding of frizzled (Fzd) receptors by their Wnt ligands results in the inhibition of beta-catenin degradation and subsequent transcription of beta-catenin/LEF-inducible genes. The beta-catenin pathway is known to be involved in development, tumorigenesis, and stem cell self-renewal. In humans, the FZD9 gene lies in the region of chromosome 7q11.23 deleted in the neurodevelopmental disorder, Williams-Beuren syndrome (WBS). Fzd9-/- mice show no obvious features of WBS, but reveal a role for Fzd9 in lymphoid development and maturation. Fzd9-/- mice show pronounced splenomegaly, thymic atrophy, and lymphadenopathy with age, with accumulation of plasma cells in lymph nodes. There is a depletion of developing B cells in the bone marrow (BM), particularly in the pre-B stage where immunoglobulin heavy chains are expressed and the cells are undergoing clonal expansion prior to light chain rearrangement. The pre-B defect is partially intrinsic to the hematopoietic system; as in competitive BM reconstitution studies, Fzd9-/- -derived BM exhibits defective B-cell development when implanted into a wild-type host. Mature B cells are present in normal numbers in lymph node and spleen. These findings suggest a role for Fzd9 signaling in lymphoid development, particularly at points where B cells undergo self-renewal prior to further differentiation.
Adenovirus-Based Transient Expression Systems for Peritoneal Membrane Research (2006)

The use of recombinant adenoviruses to genetically modify cells and tissues is now a common laboratory research tool. This technique has provided important advances in our understanding of the peritoneal membrane.
Inhibition of NO Synthesis Induces Inflammatory Changes and Monocyte Chemoattractant Protein-1 Expression in Rat Hearts and Vessels (1998)

We recently showed that chronic inhibition of NO synthesis by N(omega)-nitro-L-arginine methyl ester (L-NAME) causes coronary vascular remodeling (ie, vascular fibrosis and medial thickening) in rats. To test the hypothesis that the inhibition of NO synthesis induces inflammatory changes in the heart, we characterized the inflammatory lesions that occurred during L-NAME administration and determined whether inflammation involved the induction of monocyte chemoattractant protein-1 (MCP-1) in vivo. During the first week of L-NAME administration to Wistar-Kyoto rats, we observed a marked infiltration of mononuclear leukocytes (ED1-positive macrophages) and fibroblast-like cells (alpha-smooth muscle actin-positive myofibroblasts) into the coronary vessels and myocardial interstitial areas. These inflammatory changes were associated with the expression of proliferating cell nuclear antigen and MCP-1 (both mRNA and protein). The areas affected by inflammatory changes, as well as the expression of MCP-1 mRNA, declined after longer (28 days) treatment with L-NAME and were replaced by vascular and myocardial remodeling. Our results support the hypothesis that the inhibition of NO synthesis induces inflammatory changes in coronary vascular and myocardial tissues and involves MCP-1 expression. Results also suggest that the early stages of inflammatory changes are important in the development of later-stage structural changes observed in rat hearts.
DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection (1993)

Replaceable linear polyacrylamide (LPA) has been utilized as a sieving matrix for DNA sequencing by capillary electrophoresis (CE). Difficulties associated with cross-linked polyacrylamide gel stability have been overcome for the routine application of CE to DNA sequencing. A simple laser-induced fluorescence (LIF) detection system based on a single laser and two photomultipliers (PMT) has been adopted for this work. Sequencing information for four bases has been obtained from two fluorescent dyes and two peak height ratios, detected in two optical channels. FAM- and JOE-labeled M13 (-21) primers have been chosen because both dyes are efficiently excited with a low-power argon ion laser, can be optically separated, and exhibit minimal dye-based shifts in DNA fragment mobilities. Addition of denaturants to the electrophoresis running buffer (1 x TBE, 3.5 M urea, 30% formamide) and column operation at 32 degrees C permitted the resolution of difficult compressed sites in the sequence of phage M13mp18. Careful examination of the polymerization reaction of LPA has led to methodology that has proven to be reproducible for obtaining DNA sequencing information of M13mp18 phage for 350 nucleotides in close to 30 min.
Evaluation of the solitary pulmonary nodule. (2009)

Solitary pulmonary nodules are common radiologic findings, typically discovered incidentally through chest radiography or computed tomography of the neck, chest, and abdomen. Primary care physicians must decide how to pursue an evaluation of a nodule once it has been identified. The differential diagnosis for pulmonary nodules includes benign and malignant causes. Diameter of 8 mm or more, "ground-glass" density, irregular borders, and doubling time between one month and one year suggest malignancy. The American College of Chest Physicians recently released guidelines for the evaluation of solitary pulmonary nodules, based primarily on nodule size and patient risk factors for cancer. Algorithms for the evaluation of lesions smaller than 8 mm and those 8 mm or greater recommend different imaging follow-up regimens. Fluorodeoxyglucose-positron emission tomography can be used to aid decision making when cancer pretest probability and imaging results are discordant. Any patient with evidence of a nodule with notable growth during follow-up should undergo biopsy for identification. The rationale for closely monitoring an incidentally found pulmonary lesion is that detection and treatment of early lung cancer might lead to decreased morbidity and mortality.
MRI, Quantitative MRI, SPECT, and neuropsychological findings following carbon monoxide poisoning (1999)

Carbon monoxide (CO) poisoning has been shown to result in neuropathologic changes and cognitive impairments due to anoxia and other related biochemical mechanisms. The present study investigated brain-behaviour relationships between neuropsychological outcome and SPECT, MRI, and Quantitative magnetic resonance imaging (QMRI) in 21 patients with CO poisoning. Ninety-three per cent of the patients exhibited a variety of cognitive impairments, including impaired attention, memory, executive function, and mental processing speed. Ninety-five per cent of the patients experienced affective changes including depression and anxiety. The results from the imaging studies revealed that 38% of the patients had abnormal clinical MRI scans, 67% had abnormal SPECT scans, and 67% had QMRI findings including hippocampal atrophy and/or diffuse cortical atrophy evidenced by an enlarged ventricle-to-brain ratio (VBR). Hippocampal atrophy was also found on QMRI. SPECT and QMRI appear to be sensitive tools which can be used to identify the neuropathological changes and cerebral perfusion defects which occur following CO poisoning. Cerebral perfusion defects include frontal and temporal lobe hypoperfusion. Significant relationships existed between the various imaging techniques and neuropsychological impairments. The data from this study indicate that a multi-faceted approach to clinical evaluation of the neuropathological and neurobehavioural changes following CO poisoning may provide comprehensive information regarding the neuroanatomical and neurobehavioural effects of CO poisoning.
A Simple Indirect ELISA using F(ab')2 Fragments of Immunoglobulin (1982)

An indirect ELISA is described in which (i) virus is trapped by F(ab')2 fragments of specific IgG immobilized on a solid phase support, (ii) trapped virus is detected by intact IgG (from the same or a different antiserum) and (iii) positive reactions are identified using an enzyme conjugate specific for the Fc portion of IgG. Pepsin digestion of the Fc portion of the trapping antibody permits the use of a general purpose enzyme conjugate to discriminate between trapping and detecting antibody. Consequently, the assay requires only a single virus-specific antiserum which is often all that is available to the plant virologist. The assay was at least as sensitive for detecting small amounts of antigen as the standard double-antibody sandwich procedure and, for some viruses, two- to fourfold more sensitive. The improvement in performance resulted largely from lower and more consistent background reactions. Both assays were equally effective in revealing heterologous reactions when optimized for detecting homologous antigen. However, increased cross-reactions were obtained in the F(ab')2 procedure by the use of higher concentrations of detecting antibody. The assay is considered particularly suited for comparing antisera from different sources or of different bleeds from the same source, and for investigations involving so few tests that the effort or expense of preparing individual virus-specific conjugates is not justified.
Sequential sampling procedures for query size estimation (1992)

We provide a procedure, based on random sampling, for estimation of the size of a query result. The procedure is sequential in that sampling terminates after a random number of steps according to a stopping rule that depends upon the observations obtained so far. Enough observations are obtained so that, with a pre-specified probability, the estimate differs from the true size of the query result by no more than a prespecified amount. Unlike previous sequential estimation procedures for queries, our procedure is asymptotically efficient and requires no ad hoc pilot sample or a a priori assumptions about data characteristics. In addition to establishing the asymptotic properties of the estimation procedure, we provide techniques for reducing undercoverage at small sample sizes and show that the sampling cost of the procedure can be reduced through stratified sampling techniques.
Identification of PTHrP(12-48) as a Plasma Biomarker Associated with Breast Cancer Bone Metastasis (2013)

In summary, we present the first validated, plasma biomarker signature for diagnosis of breast cancer bone metastasis that may improve the early diagnosis of high-risk individuals.
Knockout and knockin of the beta 1 exon D define distinct roles for integrin splice variants in heart function and embryonic development (1998)

The beta1D integrin is a recently characterized isoform of the beta1 subunit that is specifically expressed in heart and skeletal muscle. In this study we have assessed the function of the beta1D integrin splice variant in mice by generating, for the first time, Cre-mediated exon-specific knockout and knockin strains for this splice variant. We show that removal of the exon for beta1D leads to a mildly disturbed heart phenotype, whereas replacement of beta1A by beta1D results in embryonic lethality with a plethora of developmental defects, in part caused by the abnormal migration of neuroepithelial cells. Our data demonstrate that the splice variants A and D are not functionally equivalent. We propose that beta1D is less efficient than beta1A in mediating the signaling that regulates cell motility and responses of the cells to mechanical stress.
Intelligent dynamic service provisioning architecture in the multimedia era (1996)

In the multimedia/B-ISDN era, it will become essential for customers to be able to design, define, and manage communication services based on their own concepts and knowledge of communications. This paper proposes a new concept of intelligent dynamic service provisioning (IDSP) offering customers this capability. Service views of customers and carriers for multimedia services, and the interfaces required to propagate information necessary for providing services are also discussed. Based on these views, we propose an architecture for the IDSP system. Finally, the basic mechanism of the IDSP system, and the technical issues related to this system are examined.
Palladium-catalyzed synthesis of cinnamylamines (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPalladium-catalyzed synthesis of cinnamylaminesNancy J. Malek and Alan E. MoormannCite this: J. Org. Chem. 1982, 47, 27, 5395–5397Publication Date (Print):December 1, 1982Publication History Published online1 May 2002Published inissue 1 December 1982https://pubs.acs.org/doi/10.1021/jo00148a033https://doi.org/10.1021/jo00148a033research-articleACS PublicationsRequest reuse permissionsArticle Views384Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Montelukast for treating seasonal allergic rhinitis: a randomized, double‐blind, placebo‐controlled trial performed in the spring (2002)

Montelukast is well tolerated and provides improvements in daytime and night-time symptoms, as well as quality of life parameters, for patients with seasonal allergic rhinitis.