gebhart/patrepeval huggingface dataset:
MUC16 (CA125): tumor biomarker to cancer therapy, a work in progress (2014)

Over three decades have passed since the first report on the expression of CA125 by ovarian tumors. Since that time our understanding of ovarian cancer biology has changed significantly to the point that these tumors are now classified based on molecular phenotype and not purely on histological attributes. However, CA125 continues to be, with the recent exception of HE4, the only clinically reliable diagnostic marker for ovarian cancer. Many large-scale clinical trials have been conducted or are underway to determine potential use of serum CA125 levels as a screening modality or to distinguish between benign and malignant pelvic masses. CA125 is a peptide epitope of a 3-5 million Da mucin, MUC16. Here we provide an in-depth review of the literature to highlight the importance of CA125 as a prognostic and diagnostic marker for ovarian cancer. We focus on the increasing body of literature describing the biological role of MUC16 in the progression and metastasis of ovarian tumors. Finally, we consider previous and on-going efforts to develop therapeutic approaches to eradicate ovarian tumors by targeting MUC16. Even though CA125 is a crucial marker for ovarian cancer, the exact structural definition of this antigen continues to be elusive. The importance of MUC16/CA125 in the diagnosis, progression and therapy of ovarian cancer warrants the need for in-depth research on the biochemistry and biology of this mucin. A renewed focus on MUC16 is likely to culminate in novel and more efficient strategies for the detection and treatment of ovarian cancer.
Assessment of the adverse effects of the acaricide amitraz:in vitroevaluation of genotoxicity (2012)

Amitraz is a formamidine widely used in Veterinary Medicine for the treatment of ectoparasites. It is a highly liposoluble compound that is quickly absorbed through the skin and mucous membranes, thus making exposure potentially dangerous for humans and animals. The aim of this study was to compare the genotoxic potential of the active constituent of the insecticide amitraz and a commercial product containing amitraz in vitro in hamster cells. The induction of primary DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay) and the apoptotic ability was examined by the Annexin V/propidium iodide staining assay. The commercial formulation significantly increased the index of DNA damage at concentrations of 2.50-3.75 µg/mL compared to the control. The active constituent only induced significant DNA damage with the highest concentration (3.75 µg/mL). Although both tested products increased the frequency of cell death, neither of them induced significant differences. Genotoxic potential is a primary risk factor for long-term effects such as carcinogenic and reproductive toxicology. Results presented here highlight the need for further investigation of the potential health risk of this veterinary medicine.
Pattern classification and scene analysis (1973)

Provides a unified, comprehensive and up-to-date treatment of both statistical and descriptive methods for pattern recognition. The topics treated include Bayesian decision theory, supervised and unsupervised learning, nonparametric techniques, discriminant analysis, clustering, preprosessing of pictorial data, spatial filtering, shape description techniques, perspective transformations, projective invariants, linguistic procedures, and artificial intelligence techniques for scene analysis.
New Developments and Applications of Bacteriocins and Peptides in Foods (2011)

There is an increased desire for sophisticated foods, whereby consumers harbor higher expectations of health-promoting benefits above basic nutrition. Moreover, there is a move from the adulteration of foods with chemical preservatives toward biopreservation. Such expectations have led scientists to identify novel approaches to satisfy both demands, which utilize bacteriocin and peptide-based solutions. The best known examples of biopreservation involve bacteriocins. However, with the exception of nisin, bacteriocins have received limited use in the food industry. Peptides can be added to foods to improve consumer health. Some of the best known examples are angiotensin I-converting enzyme (ACE)-inhibitory peptides, which inhibit ACE, a key enzyme involved in blood pressure (BP) regulation. To be effective, these peptides must be bioavailable, but by their nature, peptides are degraded by digestion with proteolytic enzymes. This review critically discusses the use and potential of peptides and bacteriocins in food systems in terms of safety, quality, and improvement of human health.
High Molecular Weight Atactic Polypropylene prepared by Zirconium Complexes of an Amine Bis(phenolate) Ligand (2002)

Abstract The polymerization of propylene employing the recently developed amine bis(phenolate) zirconium catalysts is reported for the first time. The polymerizations were conducted in liquid propylene employing two different experimental setups, and two different precatalysts, based on the same amine bis(phenolate) ligand having a dimethylamino donor on a sidearm. The first precatalyst studied was a dibenzyl zirconium complex, which was found to lead to high molecular weight atactic polypropylene. The effects of the cocatalyst, the ratio between the cocatalyst and the precatalyst, and the polymerization temperature on the nature of the resulting polymer and the activity of the catalyst were studied. Reducing the relative amount of the cocatalyst and lowering the polymerization temperature increased the molecular weight of the polymer, and a temperature of 50 °C led to the most active catalyst. A dichloro zirconium complex of the same ligand, expected to show a higher hydrolytic and thermal stability was developed, its X‐ray structure was solved, and its activity in polymerization of propylene was studied and found to be similar to that of the dibenzyl precatalyst.
miRNA expression profiles in chronic lymphocytic and acute lymphocytic leukemia (2007)

MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles by targeting mRNAs for cleavage or translational repression. miRNAs act in diverse biological processes including development, cell growth, apoptosis, and hematopoiesis, suggesting their association with cancer. We determined the miRNA expression profile of chronic and acute lymphocytic leukemias (CLL and ALL) using the TaqMan MicroRNA Assays Human Panel (Applied Biosystems). Pooled leukemia samples were compared to pooled CD19+ samples from healthy individuals (calibrator) by the 2-DD Ct method. Total RNA input was normalized based on the Ct values obtained for hsa-miR-30b. The five most highly expressed miRNAs were miR-128b, miR-204, miR-218, miR-331, and miR-181b-1 in ALL, and miR-331, miR-29a, miR-195, miR-34a, and miR-29c in CLL. To our knowledge, this is the first report associating miR-128b, miR-204 and miR-331 to hematological malignancies. The miR-17-92 cluster was also found to be up-regulated in ALL, as previously reported for some types of lymphomas. The differences observed in gene expression levels were validated for miR-331 and miR-128b in ALL and CD19+ samples. These miRNAs were up-regulated in ALL, in agreement with our initial results. A brief target analysis was performed for miR-331. One of its putative targets, SOCS1, promotes STAT activation, which is a known mediator of cell proliferation and survival, suggesting the possibility of an association between miR-331 and these processes. This initial screening provided information on miRNA differentially expressed in normal and malignant B-cells that could suggest the potential roles of these miRNAs in hematopoiesis and leukemogenesis.
Resistance against Herbicide Isoxaben and Cellulose Deficiency Caused by Distinct Mutations in Same Cellulose Synthase Isoform CESA6 (2002)

Isoxaben is a pre-emergence herbicide that inhibits cellulose biosynthesis in higher plants. Two loci identified by isoxaben-resistant mutants (ixr1-1, ixr1-2, and ixr2-1) in Arabidopsis have been reported previously. IXR1 was recently shown to encode the cellulose synthase catalytic subunit CESA3 (W.-R. Scheible, R. Eshed, T. Richmond, D. Delmer, and C. Somerville [2001] Proc Natl Acad Sci USA 98: 10079-10084). Here, we report on the cloning of IXR2, and show that it encodes another cellulose synthase isoform, CESA6. ixr2-1 carries a mutation substituting an amino acid close to the C terminus of CESA6 that is highly conserved among CESA family members. Transformation of wild-type plants with the mutated gene and not with the wild-type gene conferred increased resistance against the herbicide. The simplest interpretation for the existence of these two isoxaben-resistant loci is that CESA3 and CESA6 have redundant functions. However, loss of function procuste1 alleles of CESA6 were previously shown to have a strong growth defect and reduced cellulose content in roots and dark-grown hypocotyls. This indicates that in these mutants, the presence of CESA3 does not compensate for the absence of CESA6 in roots and dark-grown hypocotyls, which argues against redundant functions for CESA3 and CESA6. Together, these observations are compatible with a model in which CESA6 and CESA3 are active as a protein complex.
Studies on the Alkylation of Chiral, Non-racemic, Tricyclic Pyrrolidinones (1995)

Condensation of cyclic keto acids with chiral amino alcohols provides tricyclic pyrrolidinones with high levels of diastereoselectivity.These pyrrolidinones can be akylated via their lithium or sodium enolates.Optimal alkylation conditions vary with the nature of the amino alcohol; the phenylglycinol-derived pyrrolidinones suffer from an apparent benzylic metallation, whereas the valinol and norephedrine-derived substrates are good alkylation substrates.Application of this methodology to a vinylsilane / iminium ion cyclization approach to the synthesis of Whitesell's amine was also investigated.' The double alkylation product was isolated in 17% yield.
A Mitochondrial Paradigm of Metabolic and Degenerative Diseases, Aging, and Cancer: A Dawn for Evolutionary Medicine (2005)

Life is the interplay between structure and energy, yet the role of energy deficiency in human disease has been poorly explored by modern medicine. Since the mitochondria use oxidative phosphorylation (OXPHOS) to convert dietary calories into usable energy, generating reactive oxygen species (ROS) as a toxic by-product, I hypothesize that mitochondrial dysfunction plays a central role in a wide range of age-related disorders and various forms of cancer. Because mitochondrial DNA (mtDNA) is present in thousands of copies per cell and encodes essential genes for energy production, I propose that the delayed-onset and progressive course of the age-related diseases results from the accumulation of somatic mutations in the mtDNAs of post-mitotic tissues. The tissue-specific manifestations of these diseases may result from the varying energetic roles and needs of the different tissues. The variation in the individual and regional predisposition to degenerative diseases and cancer may result from the interaction of modern dietary caloric intake and ancient mitochondrial genetic polymorphisms. Therefore the mitochondria provide a direct link between our environment and our genes and the mtDNA variants that permitted our forbears to energetically adapt to their ancestral homes are influencing our health today.
Dose‐Related Safety and Immunogenicity of a Trivalent Baculovirus‐Expressed Influenza‐Virus Hemagglutinin Vaccine in Elderly Adults (2006)

Baculovirus-expressed HA is a safe and immunogenic influenza vaccine in elderly adults.
Effect of age and pattern of gain on induction of puberty with a progestin in beef heifers. (1997)

Crossbred heifers (n = 75) fed for rapid (R; .82 kg/d) or slow-then-rapid (SR; .41 kg/d for 90 d then .82 kg/d) postweaning gain were used to examine the effects of age or pattern of gain on induction of puberty by a progestin. At 9.5, 11.0, and 12.5 mo of age, 12 prepuberal heifers from each growth treatment received progestin (a 6-mg Norgestomet implant for 10 d) or control treatments. Induction of puberty, LH secretory profiles, and ovarian follicular characteristics were assessed in Norgestomet-treated and control heifers. Body weights of R heifers were greater (P < .01) than those of SR heifers at all ages. At 12.5 mo, more Norgestomet-treated heifers exhibited a puberal estrus within 5 d after implant removal compared with controls (82% vs 9%, respectively), but Norgestomet did not induce puberty at 9.5 or 11 mo of age (progestin x age, P < .05) in heifers of either gain pattern. Norgestomet increased (P < .01) LH pulse frequency at all ages, whereas Norgestomet increased only mean LH concentrations at 12.5 mo of age (progestin x age, P < .03). Norgestomet treatment altered (P < .01) ovarian follicular characteristics at all ages. Gain pattern did not affect (P > .1) LH secretory profiles, ovarian characteristics, or induction of puberty by Norgestomet. We conclude that progestins induce puberty by hastening the normal cascade of endocrine and ovarian events associated with spontaneous puberty. Furthermore, age, but not pattern of gain, seems to be the critical factor influencing the efficacy of progestins to induce puberty in heifers.
IgG Subclass-specific Antibodies in Chlamydia pneumoniae Infections (1998)

IgG subclass-specific antibodies to Chlamydia pneumoniae and chlamydial lipopolysaccharide (LPS) were analysed in paired sera obtained from 15 patients with primary C. pneumoniae pneumonia and from 16 pneumonia patients with reinfection, as well as in single sera of 40 subjects with possible chronic C. pneumoniae infection and 40 healthy controls. The microimmunofluorescence (MIF) method was used to measure total IgG, IgM and IgG subclass-specific antibodies to C. pneumoniae protein antigens and enzyme immunoassay (EIA) to measure antibodies against the LPS antigen. By MIF, IgG1 antibodies to C. pneumoniae were demonstrated in all individuals of the 3 patient groups and also in all healthy controls. IgG2 subclass antibodies were not found by MIF. IgG3 antibodies were detected in 40% of patients with primary infection, in 31% of patients with reinfection, in 25% of those with chronic infection and in 8% of the controls. IgG4 antibodies were associated with acute C. pneumoniae infection and were found in 13% of primary infections and 31% of reinfections. The subclass pattern of LPS antibodies resembled that of protein antibodies measured by MIF: IgG1 was the most common subclass among the antibodies to LPS.
What is Good Testing?: Perceptions of College Students (1993)

How do college students define good testing and under what conditions do they perceive classroom testing as worthwhile? One starting point for answering these questions is to use data from stan dardized student ratings of courses. Many of the standard rating forms contain one or more items devoted to evaluation of the methods of testing used in a course. What does it mean when student evaluations indicate that an instructor was excellent in the area of examinations? The course evaluation form that we use on our campus (the Instructional Development and Effectiveness Assess ment, or IDEA, developed at Kansas State University) includes an item on preparing examinations. A high ranking on that item seems likely to reflect students' perceptions that ex amination procedures in a class have been effective. A phone call to the IDEA office revealed that they were unaware of any studies that had tried to determine how class procedures dif fered among classes that received vari ous ratings on testing. A review of cur rent literature confirmed that little or no research has been published on the topic. I made this study, then, to deter mine what was meant when students at
Characterization and structure of genes for proteases A and B from Streptomyces griseus (1987)

Protease A and protease B are extracellular proteins which are secreted by Streptomyces griseus. The genes encoding protease A (sprA) and protease B (sprB) were isolated from an S. griseus genomic library by using a synthetic oligonucleotide probe. Fragments containing sprA and sprB were characterized by hybridization and demonstration of proteolytic activity in Streptomyces lividans. Each DNA sequence contains a large open reading frame with the coding region of the mature protease situated at its carboxy terminus. The amino terminus of each reading frame appears to encode a 38-amino-acid signal peptide followed by a 76- or 78-amino-acid polypeptide, a propeptide, which is joined to the mature protease. Strong homology between the coding regions of the protease genes suggests that sprA and sprB originated by gene duplication.
Pharmacological BACE1 and BACE2 inhibition induces hair depigmentation by inhibiting PMEL17 processing in mice (2016)

Melanocytes of the hair follicle produce melanin and are essential in determining the differences in hair color. Pigment cell-specific MELanocyte Protein (PMEL17) plays a crucial role in melanogenesis. One of the critical steps is the amyloid-like functional oligomerization of PMEL17. Beta Site APP Cleaving Enzyme-2 (BACE2) and γ-secretase have been shown to be key players in generating the proteolytic fragments of PMEL17. The β-secretase (BACE1) is responsible for the generation of amyloid-β (Aβ) fragments in the brain and is therefore proposed as a therapeutic target for Alzheimer's disease (AD). Currently BACE1 inhibitors, most of which lack selectivity over BACE2, have demonstrated efficacious reduction of amyloid-β peptides in animals and the CSF of humans. BACE2 knock-out mice have a deficiency in PMEL17 proteolytic processing leading to impaired melanin storage and hair depigmentation. Here, we confirm BACE2-mediated inhibition of PMEL17 proteolytic processing in vitro in mouse and human melanocytes. Furthermore, we show that wildtype as well as bace2(+/-) and bace2(-/-) mice treated with a potent dual BACE1/BACE2 inhibitor NB-360 display dose-dependent appearance of irreversibly depigmented hair. Retinal pigmented epithelium showed no morphological changes. Our data demonstrates that BACE2 as well as additional BACE1 inhibition affects melanosome maturation and induces hair depigmentation in mice.
WaveLAN®-II: a high-performance wireless LAN for the unlicensed band (2002)

In July 1997 the Institute of Electrical and Electronics Engineers (IEEE) completed standard 802.11 for wireless local area networks (LANs). WaveLAN®-II, to be released early in 1998, offers compatibility with the IEEE 802.11 standard for operation in the 2.4-GHz band. It is the successor to WaveLAN-I, which has been in the market since 1991. As a next-generation wireless LAN product, WaveLAN-II has many enhancements to improve performance in various areas. An IEEE 802.11 direct sequence spread spectrum (DSSS) product, WaveLAN-II supports the basic bit rates of 1 and 2 Mb/s, but it can also provide enhanced bit rates as high as 10 Mb/s. This paper discusses various aspects of the system design of WaveLAN-II and characteristics of its antenna, radio-frequency (RF) front-end, digital signal processor (DSP) transceiver chip, and medium access controller (MAC) chip.
Stage-Dependent Development of Rat 1-Cell Embryos in a Chemically Defined Medium after Fertilization in Vivo and in Vitro (1997)

The present study was conducted to examine the development of rat 1-cell embryos cultured in a chemically defined medium (mR1ECM) soon after penetration of eggs in vivo or in vitro. When eggs were recovered from naturally mated females at 0600-0900 h on the following day, no penetrated eggs with male pronuclei were observed. However, the percentage of pronuclear eggs had increased significantly at 1000-1100 h (17%) and 1200-1300 h (65%). When penetrated eggs recovered at 0600-0900 h were cultured in mR1ECM, blastocyst formation (22-46%) was significantly less frequent than in those (79-93%) recovered at 1000-1300 h. After collection at 0600-0800 h, preculture of penetrated eggs in modified Krebs-Ringer bicarbonate solution (mKRB) for up to 1200-1300 h improved their development to the blastocyst stage from 42% to 78%. Pronuclei were formed in almost all (98-100%) of the penetrated eggs examined after 6-20 h of insemination in mKRB. When penetrated eggs were transferred from mKRB into mR1ECM after 4-30 h of insemination, the percentages (47-64%) of blastocyst formation were significantly higher than those (1-20%) for eggs transferred after 1-3 h or after 40 h of insemination. When a total of 70 morulae or early blastocysts that had been produced by in vitro fertilization and developed in vitro were transferred to 7 pseudopregnant rats, 5 recipients into which 48 embryos had been transferred maintained their pregnancies and 25% of the embryos developed into late fetuses or pups. The results of the present study indicate that rat 1-cell embryos recovered from oviducts before pronuclear formation, or produced by in vitro fertilization, can develop to the blastocyst stage in vitro and that one or more factors in mKRB are necessary to maintain their development in mR1ECM.
Homogeneous asymmetric hydrogenation of functionalized ketones (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHomogeneous asymmetric hydrogenation of functionalized ketonesMasato. Kitamura, Takeshi. Ohkuma, Shinichi. Inoue, Noboru. Sayo, Hidenori. Kumobayashi, Susumu. Akutagawa, Tetsuo. Ohta, Hidemasa. Takaya, and Ryoji. NoyoriCite this: J. Am. Chem. Soc. 1988, 110, 2, 629–631Publication Date (Print):January 1, 1988Publication History Published online1 May 2002Published inissue 1 January 1988https://pubs.acs.org/doi/10.1021/ja00210a070https://doi.org/10.1021/ja00210a070research-articleACS PublicationsRequest reuse permissionsArticle Views5728Altmetric-Citations469LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Ectopic Expression of Cancer–Testis Antigens in Cutaneous T-cell Lymphoma Patients (2014)

A number of CT genes are ectopically expressed in patients with CTCL and can be used as biomarkers or novel targets for immunotherapy.
High‐resolution T1 and T2 mapping of the brain in a clinically acceptable time with DESPOT1 and DESPOT2 (2004)

Variations in the intrinsic T(1) and T(2) relaxation times have been implicated in numerous neurologic conditions. Unfortunately, the low resolution and long imaging time associated with conventional methods have prevented T(1) and T(2) mapping from becoming part of routine clinical evaluation. In this study, the clinical applicability of the DESPOT1 and DESPOT2 imaging methods for high-resolution, whole-brain, T(1) and T(2) mapping was investigated. In vivo, 1-mm(3) isotropic whole-brain T(1) and T(2) maps of six healthy volunteers were acquired at 1.5 T with an imaging time of <17 min each. Isotropic maps (0.34 mm(3)) of one volunteer were also acquired (time <21 min). Average signal-to-noise within the 1-mm(3) T(1) and T(2) maps was approximately 20 and approximately 14, respectively, with average repeatability standard deviations of 46.7 ms and 6.7 ms. These results demonstrate the clinical feasibility of the methods in the study of neurologic disease.