gebhart/patrepeval huggingface dataset:
Zero Duality Gap in Optimal Power Flow Problem (2011)

The optimal power flow (OPF) problem is nonconvex and generally hard to solve. In this paper, we propose a semidefinite programming (SDP) optimization, which is the dual of an equivalent form of the OPF problem. A global optimum solution to the OPF problem can be retrieved from a solution of this convex dual problem whenever the duality gap is zero. A necessary and sufficient condition is provided in this paper to guarantee the existence of no duality gap for the OPF problem. This condition is satisfied by the standard IEEE benchmark systems with 14, 30, 57, 118, and 300 buses as well as several randomly generated systems. Since this condition is hard to study, a sufficient zero-duality-gap condition is also derived. This sufficient condition holds for IEEE systems after small resistance (10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-5</sup> per unit) is added to every transformer that originally assumes zero resistance. We investigate this sufficient condition and justify that it holds widely in practice. The main underlying reason for the successful convexification of the OPF problem can be traced back to the modeling of transformers and transmission lines as well as the non-negativity of physical quantities such as resistance and inductance.
Forthcoming receptor tyrosine kinase inhibitors (2006)

Receptor tyrosine kinases play a significant role in carcinogenesis and have been successfully targeted with monoclonal antibodies and small-molecule inhibitors. There have been recent developments in the understanding of receptor tyrosine kinase signal transduction which have enabled better drug development. The use of receptor tyrosine kinase inhibitors in clinical practice has expanded the knowledge on cancer biology, in particular the understanding of resistant mutations and strategies to overcome such resistance. This has driven drug development from single kinase inhibitors to multi-kinase inhibitors and high-affinity kinase inhibitors. Finally, as the use of receptor tyrosine kinase inhibitors grows in clinical practice, more is learned about appropriate patient selection for such therapies. This is an exciting time in cancer therapeutics, highlighted by the advent of effective targeted therapy with receptor tyrosine kinase inhibitors.
Granulocyte–Macrophage Progenitors as Candidate Leukemic Stem Cells in Blast-Crisis CML (2004)

Activation of beta-catenin in CML granulocyte-macrophage progenitors appears to enhance the self-renewal activity and leukemic potential of these cells.
Silencing of microRNA-155 in mice during acute inflammatory response leads to derepression of c/ebp Beta and down-regulation of G-CSF (2009)

microRNA-155 (miR-155) has been implicated as a central regulator of the immune system, but its function during acute inflammatory responses is still poorly understood. Here we show that exposure of cultured macrophages and mice to lipopolysaccharide (LPS) leads to up-regulation of miR-155 and that the transcription factor c/ebp Beta is a direct target of miR-155. Interestingly, expression profiling of LPS-stimulated macrophages combined with overexpression and silencing of miR-155 in murine macrophages and human monocytic cells uncovered marked changes in the expression of granulocyte colony-stimulating factor (G-CSF), a central regulator of granulopoiesis during inflammatory responses. Consistent with these data, we show that silencing of miR-155 in LPS-treated mice by systemically administered LNA-antimiR results in derepression of the c/ebp Beta isoforms and down-regulation of G-CSF expression in mouse splenocytes. Finally, we report for the first time on miR-155 silencing in vivo in a mouse inflammation model, which underscores the potential of miR-155 antagonists in the development of novel therapeutics for treatment of chronic inflammatory diseases.
A Genetic Study of Total IgE Levels in the Amish (1982)

Relevant to the investigation of genetic factors involved in allergic response is the mode of inheritance of IgE levels, an important factor in allergic disease. Serum total IgE levels in 208 Pennsylvania Amish individuals from 23 nuclear families were studied. Males had a significantly higher mean in IgE level than females. There was also a significant negative relationship between age and IgE levels. Segregation analysis was used to fit a major gene model to the age- and sex-adjusted data. The environmental model was rejected (x2 = 41.12, p less than 0.005). The Mendelian codominant model was not rejected x2 = 8.84, p approximately or equal to 0.03) and gave a better fit to the data than the Mendelian dominant or recessive model.
Recent sedimentary history of Lake Mendota, Wis (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRecent sedimentary history of Lake Mendota, WisGilbert C. Bortleson and G. Fred LeeCite this: Environ. Sci. Technol. 1972, 6, 9, 799–808Publication Date (Print):September 1, 1972Publication History Published online1 May 2002Published inissue 1 September 1972https://pubs.acs.org/doi/10.1021/es60068a002https://doi.org/10.1021/es60068a002research-articleACS PublicationsRequest reuse permissionsArticle Views163Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Enzymic determination of d(−)-β-hydroxybutyric acid and acetoacetic acid in blood (1962)

Research Article| January 01 1962 Enzymic determination of d(−)-β-hydroxybutyric acid and acetoacetic acid in blood DH WILLIAMSON; DH WILLIAMSON Search for other works by this author on: This Site PubMed Google Scholar J MELLANBY; J MELLANBY Search for other works by this author on: This Site PubMed Google Scholar HA KREBS HA KREBS Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1962) 82 (1): 90–96. https://doi.org/10.1042/bj0820090 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation DH WILLIAMSON, J MELLANBY, HA KREBS; Enzymic determination of d(−)-β-hydroxybutyric acid and acetoacetic acid in blood. Biochem J 1 January 1962; 82 (1): 90–96. doi: https://doi.org/10.1042/bj0820090 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1962 The Biochemical Society1962 Article PDF first page preview Close Modal You do not currently have access to this content.
Compressive properties and function—composition relationships of developing bovine articular cartilage (2001)

The composition of cartilage is known to change during fetal and postnatal development. The objectives of this study were to characterize the compressive biomechanical properties of the 1 mm thick articular layer of cartilage of the distal femur from third-trimester bovine fetuses, from 1 to 3 week old bovine calf and from young adult bovine knees, and to correlate these properties with tissue components. The confined compression modulus increased 180% from the fetus to the calf and adult. The hydraulic permeability at 45% offset compression (relative to the free-swelling thickness) decreased by 70% from fetus to adult. These development-associated changes in biomechanical properties were primarily associated with a marked (approximately 2-3-fold) increase during development in collagen content and no detectable change in glycosaminoglycan (GAG) content. A role for collagen in the compressive properties of cartilage and the gradual increase in collagen during development suggest that collagen metabolism is critical for cartilage tissue engineering and repair therapies.
In vitro studies on the control of nerve fiber growth by the extracellular matrix of the nervous system. (1987)

1. Cultured neurons from embryonic chick sympathetic ganglia or dorsal root ganglia grow nerve fibers extensively on simple substrata containing fibronectin, collagens (types I, III, IV), and especially laminin. 2. The same neurons cultured on substrata containing glycosaminoglycans grow poorly. Glycosaminoglycans (heparin) inhibit nerve fiber growth on fibronectin substrata. 3. Proteolytic fragments of fibronectin support nerve fiber growth only when the cell attachment region is intact. For example, a 105 kD fragment, encompassing the cell attachment region, supports growth when immobilized in a substratum, but a 93 kD subfragment, lacking the cell attachment region, is unable to support fiber growth. When it is added to the culture medium, the 105 kD fragment inhibits fiber growth on substrata containing native fibronectin. 4. In culture medium lacking NGF, DRG neurons extend nerve fibers only on laminin and not on fibronectin, collagen or polylysine. Studies with radioiodinated laminin indicate that laminin binds with a relatively high affinity (kd approximately equal to 10(-9) M) to DRG neurons, and to a variety of other neural cells (NG108 cells, PC12 cells, rat astrocytes, chick optic lobe cells). We have isolated a membrane protein (67 kD) by affinity chromatography on laminin columns and are characterizing this putative laminin receptor. 5. Dissociated DRG neurons or ganglionic explants cultured on complex substrata consisting of tissue sections of CNS or PNS tissues extend nerve fibers onto the PNS (adult rat sciatic nerve) but not CNS (adult rat optic nerve) substrata. Other tissue substrata which support fiber growth in vivo (embryonic rat spinal cord, goldfish optic nerve) support growth in culture. While substrata from adult CNS, which support meager regeneration in vivo (adult rat spinal cord) support little fiber growth in culture. 6. Ganglionic explants cultured in a narrow space between a section of rat sciatic nerve and optic nerve grow preferentially onto the sciatic nerve suggesting that diffusible growth factors are not responsible for the differential growth on the two types of tissues. 7. Dissociated neurons adhere better to sections of sciatic nerve than optic nerve. Laminin, rather than fibronectin or heparan sulfate proteoglycan, is most consistently identifiable by immunocytochemistry in tissues (sciatic nerve, embryonic spinal cord, goldfish optic nerve) which support nerve fiber growth. Taken together, these data suggest that ECM adhesive proteins are important determinants of nerve regeneration.
Optimization of Expression Conditions Enhances Production of Sepiapterin, a Precursor for Tetrahydrobiopterin Biosynthesis, in Recombinant Escherichia coli (2015)

Sepiapterin is a precursor for the synthesis of tetrahydrobiopterin (BH4), which is a wellknown cofactor for aromatic amino acid hydroxylation and nitric oxide synthesis in higher mammals. In this study, a recombinant Escherichia coli BL21(DE3) strain harboring cyanobacterial guanosine 5'-triphosphate cyclohydrolase 1 (GCH1) and human 6- pyruvoyltetrahydropterin synthase (PTPS) genes was constructed to produce sepiapterin. The optimum conditions for T7 promoter-driven expression of GCH1 and PTPS were 30°C and 0.1 mM isopropyl-β-D-thioglucopyranoside (IPTG). The maximum sepiapterin concentration of 88.1 ± 2.4 mg/l was obtained in a batch cultivation of the recombinant E. coli, corresponding to an 18-fold increase in sepiapterin production compared with the control condition (37°C and 1 mM IPTG).
Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin (1991)

Concentrated broth culture supernatants from 50 to 60% of Helicobacter pylori strains induce eukaryotic cell vacuolation in vitro. A quantitative assay for cell vacuolation was developed on the basis of the rapid uptake of visibly vacuolated HeLa cells was significantly greater than that of nonvacuolated cells. By using the rapid NRU assay, we sought to determine the roles of H. pylori cytotoxin, urease, and ammonia in the vacuolation of HeLa cells. The NRU of HeLa cells incubated in medium containing ammonium chloride or ammonium sulfate was significantly greater than that of cells incubated in medium alone. In addition, ammonium salts augmented the NRU induced by H. pylori supernatants. The NRU induced by jack bean urease was augmented by the addition of urea to cell culture medium; this suggests that urease-mediated NRU occurs via the generation of ammonia. Acetohydroxamic acid blocked the NRU induced by jack bean urease and urea but failed to block the uptake induced by H. pylori supernatants. Supernatant from a non-urease-producing H. pylori mutant strain induced NRU identical to that of the urease-positive parental strain. These observations indicate that the vacuolating activity in H. pylori supernatants is not mediated solely by urease activity but that it may be potentiated by urease-mediated ammonia production.
An integrative variant analysis suite for whole exome next-generation sequencing data (2012)

We have implemented the Atlas2 Suite and applied it to 92 whole exome samples from the 1000 Genomes Project. The Atlas2 Suite is available for download at http://sourceforge.net/projects/atlas2/. In addition to a command line version, the suite has been integrated into the Genboree Workbench, allowing biomedical scientists with minimal informatics expertise to remotely call, view, and further analyze variants through a simple web interface. The existing genomic databases displayed via the Genboree browser also streamline the process from variant discovery to functional genomics analysis, resulting in an off-the-shelf toolkit for the broader community.
Mechanism of the Ca2+-induced Enhancement of the Intrinsic Factor VIIa Activity (2008)

The intrinsic activity of coagulation factor VIIa (FVIIa) is dependent on Ca(2+) binding to a loop (residues 210-220) in the protease domain. Structural analysis revealed that Ca(2+) may enhance the activity by attenuating electrostatic repulsion of Glu(296) and/or by facilitating interactions between the loop and Lys(161) in the N-terminal tail. In support of the first mechanism, the mutations E296V and D212N resulted in similar, about 2-fold, enhancements of the amidolytic activity. Moreover, mutation of the Lys(161)-interactive residue Asp(217) or Asp(219) to Ala reduced the amidolytic activity by 40-50%, whereas the K161A mutation resulted in 80% reduction. Hence one of these Asp residues in the Ca(2+)-binding loop appears to suffice for some residual interaction with Lys(161), whereas the more severe effect upon replacement of Lys(161) is due to abrogation of the interaction with the N-terminal tail. However, Ca(2+) attenuation of the repulsion between Asp(212) and Glu(296) keeps the activity above that of apoFVIIa. Altogether, our data suggest that repulsion involving Asp(212) in the Ca(2+)-binding loop suppresses FVIIa activity and that optimal activity requires a favorable interaction between the Ca(2+)-binding loop and the N-terminal tail. Crystal structures of tissue factor-bound FVIIa(D212N) and FVIIa(V158D/E296V/M298Q) revealed altered hydrogen bond networks, resembling those in factor Xa and thrombin, after introduction of the D212N and E296V mutations plausibly responsible for tethering the N-terminal tail to the activation domain. The charge repulsion between the Ca(2+)-binding loop and the activation domain appeared to be either relieved by charge removal and new hydrogen bonds (D212N) or abolished (E296V). We propose that Ca(2+) stimulates the intrinsic FVIIa activity by a combination of charge neutralization and loop stabilization.
Kinematics and Optimization of a Spatial 3-UPU Parallel Manipulator (1999)

The structural characteristics associated with parallel manipulators are investigated. Using these characteristics a class of 3 degree-of-freedom parallel manipulators are enumerated. Several parallel manipulators with only translational degrees of freedom are identified and the 3-UPU parallel manipulator is chosen for design analysis and optimization. The kinematics of this 3-UPU parallel manipulator is studied. Two geometric conditions that lead to pure translational motion of the moving platform are described. Due to the simple kinematic structure, the inverse kinematics yields two equal and opposite limb lengths whereas the direct kinematics produces two possible manipulator postures with one being the mirror image of the other. The Jacobian matrix is derived and several singular conditions are discussed. Furthermore the conditions for existence of an isotropic point within the workspace are discussed and equations to compute the isotropic configurations of a 3-UPU manipulator are derived. Finally, we undertake architecture optimization and show that certain values of design variables maximize the global condition index of the 3-UPU manipulator. [S1050-0472(00)01404-5]
Plasma reactive oxygen species activity and antioxidant potential levels in rosacea patients: correlation with seropositivity to Helicobacter pylori (2004)

Our results suggest that rosacea is an oxidative stress condition, as reflected by the increased ROS activity and decreased AOP, regardless of HP infection.
The B Cell Antigen Receptor Complex: Association of Ig-α and Ig-β with Distinct Cytoplasmic Effectors (1992)

The B cell antigen receptor complex is a hetero-oligomeric structure composed of antigen binding, membrane immunoglobulin, and transducer-transporter substructures. The transducer-transporter substructure is composed of disulfide-linked dimers of immunoglobulin (Ig)-alpha and Ig-beta/gamma subunits that are products of the mb-1(alpha) and B29 (beta/gamma) genes. Although the receptor complex associates with Src family kinases that are activated after receptor ligation, the site of interaction of these and other cytoplasmic effector molecules with receptor subunits is unknown. The cytoplasmic tails of Ig-alpha and Ig-beta chains were found to associate with distinct sets of effector molecules. The Ig-alpha chain cytoplasmic domain bound to the Src family kinases Lyn and Fyn, phosphatidylinositol-3 kinase (PI-3 kinase), and an unidentified 38-kilodalton phosphoprotein; the cytoplasmic tail of Ig-beta bound PI-3 kinase and unidentified 40- and 42-kilodalton phosphoproteins. Binding activity was found to occur within a 26-amino acid sequence of Ig-alpha and Ig-beta that contains a motif [(Asp or Glu)-(any amino acid)7-(Asp or Glu)-Tyr-(any amino acid)3-Leu-(any amino acid)7-Tyr-(any amino acid)2-(Leu or Ile)] previously implicated in signal transduction via other receptors including the Fc epsilon receptor I and the T cell antigen receptor. These findings indicate that the subunits act independently to activate distinct second messenger pathways.
Solid-Phase Synthesis of Doubly Labeled Peptide Nucleic Acids as Probes for the Real-Time Detection of Hybridization (2000)

A substantial fluorescence enhancement was observed when novel peptide nucleic acid (PNA) probes were hybridized with complementary DNA in homogeneous solution. For the synthesis of the doubly labeled PNA probes, a highly flexible strategy was developed (see scheme). All reactions, including the labeling steps, were performed on the solid phase. Bhoc=benzhydryloxycarbonyl; Boc=tBuOCO.
Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity (2011)

Epithelial injury is a central event in the pathogenesis of many inflammatory and fibrotic lung diseases like acute respiratory distress syndrome, pulmonary fibrosis, and iatrogenic lung injury. Mechanical stress is an often underappreciated contributor to lung epithelial injury. Following injury, differentiated epithelia can assume a myofibroblast phenotype in a process termed epithelial to mesenchymal transition (EMT), which contributes to aberrant wound healing and fibrosis. We demonstrate that cyclic mechanical stretch induces EMT in alveolar type II epithelial cells, associated with increased expression of low molecular mass hyaluronan (sHA). We show that sHA is sufficient for induction of EMT in statically cultured alveolar type II epithelial cells and necessary for EMT during cell stretch. Furthermore, stretch-induced EMT requires the innate immune adaptor molecule MyD88. We examined the Wnt/β-catenin pathway, which is known to mediate EMT. The Wnt target gene Wnt-inducible signaling protein 1 (wisp-1) is significantly up-regulated in stretched cells in hyaluronan- and MyD88-dependent fashion, and blockade of WISP-1 prevents EMT in stretched cells. In conclusion, we show for the first time that innate immunity transduces mechanical stress responses through the matrix component hyaluronan, and activation of the Wnt/β-catenin pathway.
Synthese von zwei diastereomeren natürlichen Dihydroxyprolinen: 2,3‐trans‐3,4‐trans‐3,4‐Dihydroxy‐L‐prolin und 2,3‐cis‐3,4‐trans‐3,4‐Dihydroxy‐L‐prolin (1981)

Abstract Ausgehend von 2‐Pyrrolcarbonsäure wird über 3,4‐Dehydro‐D,L‐prolin ( 5 ) das N ‐Boc‐Derivat 6 durch Kristallisation mit R ( + )‐1‐(4‐Nitrophenyl)ethanamin in die Antipoden gespalten. Der daraus zugängliche N ‐Tosyl‐3,4‐dehydro‐L‐prolin‐methylester (L‐7) ergibt mit Trifluorperessigsäure die 3,4‐Epoxyester 8a und 8b , die bei der Hydrolyse eine Mischung aus dem N ‐Tosyl‐3,4‐dihydroxy‐L‐prolinester ( 9a ) und der Säure 10b ergeben. Die Säure 10b wird durch Extraktion mit Hydrogencarbonatlösung vom diastereomeren Ester 9a getrennt. Die Trennung gelingt auch durch Reaktion mit Chlorameisensäure‐isobutylester, wobei 9b unverändert bleibt und 10b ein γ‐Lacton bildet. Nach Öffnung des Lactonrings resultieren mit Natrium in flüssigem Ammoniak die Dihydroxy‐L‐proline 3 (aus 9b ) und 4 (aus 10b ), die mit natürlichem Material in allen Eigen‐schaften übereinstimmen.