gebhart/patrepeval huggingface dataset:
Truncated forms of DNA-binding estrogen receptors in human breast cancer. (1991)

The likelihood a breast cancer will respond to antiestrogen therapy depends on the tumor content of immunoreactive or ligand-binding estrogen receptor (ER). To investigate the failure of many ER-positive breast cancers to respond to antiestrogen therapy, we examined by gel-shift assay the ability of tumor ER to bind its cognate estrogen response element (ERE). Analysis of 38 primary breast cancers showed that some tumors containing abundant immunoreactive ER failed to demonstrate DNA binding ER. In many other ER-positive tumors, the fraction of DNA binding ER was low and consisted primarily of truncated receptor forms, which on Western analysis were revealed to be 50 kD homodimers and 67-50 kD ER heterodimers. The use of protease inhibitors during tumor extraction and the demonstration of nuclear-localizing ER and ERE-binding COUP (chicken ovalbumin upstream promoter) protein in these tumors indicated that the truncated forms of ER were likely present in vivo. The presence of intact DNA binding ER correlated with higher tumor content of immunoreactive sex steroid receptors (ER and/or PR), standard predictors of tumor responsiveness to antiestrogen, suggesting that loss or truncation of DNA binding ER may be an important prognostic parameter accounting for some forms of clinical resistance to antiestrogen therapy.
Cloning and Characterization of KCC3 and KCC4, New Members of the Cation-Chloride Cotransporter Gene Family (1999)

The K+-Cl- cotransporters (KCCs) belong to the gene family of electroneutral cation-chloride cotransporters, which also includes two bumetanide-sensitive Na+-K+-2Cl- cotransporters and a thiazide-sensitive Na+-Cl- cotransporter. We have cloned cDNAs encoding mouse KCC3, human KCC3, and human KCC4, three new members of this gene family. The KCC3 and KCC4 cDNAs predict proteins of 1083 and 1150 amino acids, respectively. The KCC3 and KCC4 proteins are 65-71% identical to the previously characterized transporters KCC1 and KCC2, with which they share a predicted membrane topology. The four KCC proteins differ at amino acid residues within key transmembrane domains and in the distribution of putative phosphorylation sites within the amino- and carboxyl-terminal cytoplasmic domains. The expression of mouse KCC3 in Xenopus laevis oocytes reveals the expected functional characteristics of a K+Cl- cotransporter: Cl--dependent uptake of 86Rb+ which is strongly activated by cell swelling and weakly sensitive to furosemide. A direct functional comparison of mouse KCC3 to rabbit KCC1 indicates that KCC3 has a much greater volume sensitivity. The human KCC3 and KCC4 genes are located on chromosomes 5p15 and 15q14, respectively. Although widely expressed, KCC3 transcripts are the most abundant in heart and kidney, and KCC4 is expressed in muscle, brain, lung, heart, and kidney. The unexpected molecular heterogeneity of K+-Cl- cotransport has implications for the physiology and pathophysiology of a number of tissues.
Large scale models of cooperative and hostile intentions (2002)

Large-scale computer models of intentions have the potential to play a significant role in the coordination of large, complex macro-systems. By representing the intentions of all participants in a unified, dynamic, real-time computer model, participants can detect conflicts between their activities and those of others. In a cooperating macro-system, participants can improve overall performance by adapting their activities to reduce conflict and enhance cooperation. In adversarial macrosystems, such as military combat, tracking the intentions of friendly and hostile forces can provide enhanced counter-planning and response execution. This paper provides a brief view of the current progress in constructing and testing large scale models of intent based on recent manned simulation experiments and demonstrations.
Not So Healthy Libraries?: Report on the 2003-2004 Statistical Survey of Victorian Health Sciences Libraries (2005)

The key findings of the 2003-2004 Statistical Survey of Victorian Health Sciences Libraries are discussed. Libraries need to keep a close watch on resource and productivity levels in their core business areas as evidence is beginning to emerge of a decline in the demand for in-house library services, probably due to competition from other providers.
Effects of the obese Gene Product on Body Weight Regulation in ob / ob Mice (1995)

C57BL/6J mice with a mutation in the obese (ob) gene are obese, diabetic, and exhibit reduced activity, metabolism, and body temperature. Daily intraperitoneal injection of these mice with recombinant OB protein lowered their body weight, percent body fat, food intake, and serum concentrations of glucose and insulin. In addition, metabolic rate, body temperature, and activity levels were increased by this treatment. None of these parameters was altered beyond the level observed in lean controls, suggesting that the OB protein normalized the metabolic status of the ob/ob mice. Lean animals injected with OB protein maintained a smaller weight loss throughout the 28-day study and showed no changes in any of the metabolic parameters. These data suggest that the OB protein regulates body weight and fat deposition through effects on metabolism and appetite.
Effect of nursing interventions on patients with chronic heart failure (2011)

Objective To discuss the effect of nursing interventions on rehabilitation of patients with chronic heart failure.Methods Forty-six patients with chronic heart failure were randomized into two groups: trial and control group.The patients in control group were taken general nursing,while the patients in trial group were taken related nursing interventions.Results The symptoms of heart failure in patients in trial group were improved significantly compared with control group.Conclusion On the basis of modern drug therapy,earlier nursing interventions have an important role in improving patients' prognosis and adapting to the society.
Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS (1991)

The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in conjunction with the polymerase binding region for transcriptional stimulation. Interestingly, a mutant TFIIS with only the polymerase binding domain actually inhibits transcription, whereas a mutant in which the polymerase binding and zinc finger domains are separated by an octapeptide is only weakly active. The zinc finger itself has no effect on transcription, but in contrast to the wild-type protein, it binds to oligonucleotides. These findings suggest that TFIIS may interact with RNA polymerase II such that the normally masked zinc finger can specifically contact nucleotides in the transcription elongation zone at a position juxtaposed to the polymerization site.
Neuroadapted Yellow Fever Virus 17D: Determinants in the Envelope Protein Govern Neuroinvasiveness for SCID Mice (2003)

A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and 2 substitutions in the 3' untranslated region (UTR). The virulence determinants of SPYF-MN for SCID mice were identified by constructing and characterizing intratypic viruses in which the E protein of SPYF-MN was expressed in the YF5.2iv background (SPYF-E) or the E protein of YF5.2iv was expressed in the SPYF-MN background (YF5.2-E). SPYF-E caused lethal encephalitis in young adult SCID mice after intraperitoneal inoculation, with average survival times and tissue virus burdens resembling those of mice inoculated with the parental SPYF-MN virus. To define which domains of the E protein are involved in neuroinvasiveness, two viruses were tested in which the amino acid substitutions in domains I-II and III were segregated. This revealed that substitutions in domain III (residues 305, 326, and 380) were critical for the neuroinvasive phenotype, based on average survival times and tissue burdens of infectious virus. Comparison of growth properties of the various intratypic viruses in cell culture indicated that no inherent defects in replication efficiency were likely to account for the biological differences observed in these experiments. These findings demonstrate that the E protein is a critical factor for yellow fever virus neuropathogenesis in the SCID mouse model and that the neuroinvasive properties depend principally on functions contributed by domain III of this protein. To assess whether critical determinants for neuroinvasion of normal ICR mice by SPYF virus were also in the E protein, sequences of viruses recovered from brains of ICR mice succumbing to encephalitis with the parental SPYF virus were derived. No differences were found in the E protein; however, two substitutions were present in the 3' UTR compared to that of SPYF-MN, one of which is predicted to alter RNA secondary structure in this region. These findings suggest that the 3' UTR may also affect neuroinvasiveness of SPYF virus in the mouse model.
Caries Prevalence in 6- to 15-year-old French Children based on the 1987 and 1991 National Surveys (1993)

The caries prevalence in a multi-stage probability sample of 18,786 children representative of all French children 6-15 years of age was studied in 1991 and compared with the results of a similar survey made in 1987. A significant decline in dental caries in France became evident. This decline, observed in primary as well as in permanent teeth, was of variable magnitude among the different age groups. At the age of 6, 48.6% of the children were totally caries-free in 1991. At the age of 12, the DMFT and DMFS indices were 2.59 and 4.72, respectively, representing a corresponding decrease of 38% and 37%. In 1991, more caries-affected teeth or surfaces were filled, whereas fewer caries-affected teeth or surfaces were untreated. Females and children living in rural areas had a higher caries prevalence in both surveys. Pit-and-fissure lesions were the predominant caries types. Dental fluorosis was very uncommon; 96.1% of the 18,786 children examined in 1991 were totally free of any such lesions. Average plaque and calculus indices were similar in 1987 and in 1991, but a decrease of 25% was observed in the average gingival indices. Although the relative contributions of various preventive factors leading to this caries reduction are not clear, it should be noted that use of fluoridated salt (250 mg/kg KF) started in January, 1987. The sale of fluoridated dentifrices and the use of fluoride tablets and sugar substitutes increased during the period from 1987 to 1991.
Shape, Vibrations, and Effective Surface Tension of Water Marbles (2009)

The surface of water “marbles” obtained with hydrophobic lycopodium and polyvinylidene fluoride particles was investigated first with environmental scanning electron microscopy. The shape of water marbles was studied both experimentally and theoretically. The mathematical model describing the deformation of marbles by gravity is proposed. The model allowed the calculation of the effective surface tension of marbles and gives 0.09 J/m2 for marbles coated with PVDF and 0.06 J/m2 for marbles coated with lycopodium. The effective surface tensions of marbles calculated independently by the horizontal vibration of marbles were in semiquantitative agreement with the above values (0.07 J/m2 for marbles coated with PVDF and 0.055 J/m2 for marbles coated with lycopodium).
A comparison of mechanisms for improving TCP performance over wireless links (1997)

Reliable transport protocols such as TCP are tuned to perform well in traditional networks where packet losses occur mostly because of congestion. However, networks with wireless and other lossy links also suffer from significant losses due to bit errors and handoffs. TCP responds to all losses by invoking congestion control and avoidance algorithms, resulting in degraded end-to end performance in wireless and lossy systems. We compare several schemes designed to improve the performance of TCP in such networks. We classify these schemes into three broad categories: end-to-end protocols, where loss recovery is performed by the sender; link-layer protocols that provide local reliability; and split-connection protocols that break the end-to-end connection into two parts at the base station. We present the results of several experiments performed in both LAN and WAN environments, using throughput and goodput as the metrics for comparison. Our results show that a reliable link-layer protocol that is TCP-aware provides very good performance. Furthermore, it is possible to achieve good performance without splitting the end-to-end connection at the base station. We also demonstrate that selective acknowledgments and explicit loss notifications result in significant performance improvements.
Diversity of Capsular Polysaccharide Synthesis Gene Clusters in Streptococcus pneumoniae (1998)

Streptococcus pneumoniae comprises 90 serotypes, each one having its own specific polysaccharide capsule. In order to explore the diversity of capsular polysaccharide synthesis (cps) gene clusters in S. pneumoniae, we performed cross-hybridizations between the 12 cps genes of S. pneumoniae serotype 14 and chromosomal DNA of 26 strains comprising 26 different capsule types. Large variations in the hybridization patterns were observed. The genes cps14A to cps14D are conserved in most serotypes. Sequences homologous to cps14I to cps14L were only observed in the four types of serogroup 15, which all have a capsule structure similar to that of type 14. By using a cps14E knock-out construct, cpsE mutants of the pneumococcal types 9N, 13, and 15B were obtained. These mutants were unencapsulated and showed reduced glycosyltransferase activity, indicating that the pneumococcal types 9N, 13, and 15B express a glucosyl-1-phosphate transferase which is homologous to Cps14E. Glycosyltransferase assays showed that among 21 pneumococcal types which contain glucose in the core of their capsule polysaccharide, 19 types express glucosyl-1-phosphate transferase activity. However, not all of these types hybridized strongly with Cps14E, the type 14 glucosyl-1-phosphate transferase gene. Thus, pneumococci possess glucosyltransferase genes distinct from cps14E, but encoding enzymes with identical activity. All serotypes which synthesized lipid-linked lactose intermediates in glycosyltransferase activity assays (type 11B, 13, 15F, 15A, 15B, 15C) hybridized with cps14G. This gene encodes a galactosyltransferase which catalyzes the addition of 1,4-linked beta-galactose to lipid-linked glucose. The cps14G homologues in type 11B, 13, 15F, 15A, 15B, and 15C may encode a similar beta-galactosyltransferase activity as cps14G in type 14.
Exogenous Fluorescent Agents for the Determination of Glomerular Filtration Rate (2012)

Glomerular filtration rate (GFR) is now widely accepted as the best indicator of renal function in the state of health and illness.1,2 Current clinical guidelines advocate its use in the staging of chronic kidney disease as well as in assessing the risk of kidney failure under acute clinical, physiological, and pathological conditions.3-6 Acute renal failure (ARF) is a major cause of complications in the post-surgical and post-intervention vascular and cardiac procedure patient populations. ARF is also a major public health issue because it may lead to chronic renal failure. Real-time, continuous monitoring of GFR in patients at the bedside is particularly important in the case of critically ill or injured patients, and those undergoing organ transplantation because most of these patients face the risk of multiple organ failure (MOF) resulting in death.7-10 MOF is a sequential failing of lung, liver, and kidneys and is incited by one or more severe causes such as acute lung injury (ALI), adult respiratory distress syndrome (ARDS), hypermetabolism, hypotension, persistent inflammation, or sepsis. The transition from early stages of trauma to clinical MOF is marked by the extent of liver and renal failure and a change in mortality risk from about 30% to about 50%.10 Accurate determination of GFR is also necessary for monitoring patients undergoing cancer chemotherapy with nephrotoxic anticancer drugs,11 or those at risk for contrast media induced nephropathy (CIN).12 Finally, GFR measurement is also useful for patients with chronic illness such as diabetes, hypertension, obesity, hyperthyroidism, cystic fibrosis, etc. who are at risk for renal impairment.13-15
Optical wave-front transformer using the multiple-reflection interference effect inside a resonator (1997)

We propose a new class of optical wave-front transformer based on a different mechanism that uses the multiple-reflection interference effect in a Gires-Tournois resonator (GTR) as the physical mechanism for phase modification. By coating the front surface of a GTR with a predefined graded reflectivity profile, one can synthesize various optical elements. We present the basic concept of our proposal by synthesizing a lenslike element as a proof-of-principle example. One unique feature of this element is that it can function as a reflecting mirror, a converging lens, or a diverging lens, depending on the resonator length. Other applications are briefly discussed.
HER2-Specific Affibody-Conjugated Thermosensitive Liposomes (Affisomes) for Improved Delivery of Anticancer Agents (2008)

Thermosensitive liposomes are attractive vehicles for the delivery and release of drugs to tumors. To improvethe targeting efficacy for breast cancer treatment, an 8.3-kDa HER2-specific Affibody molecule (Z(HER2:342)-Cys) was conjugated to the surface of liposomes. The effects of this modification on physical characteristics and stability of the resulting nanoparticles denoted as "Affisomes" were investigated. Thermosensitive small unilamellar vesicle (SUV) liposomes of (80-100 nm) a diameter consisting of dipalmitoyl phosphatidylcholine (DPPC, Tm 41 degrees C) as the matrix lipid and a maleimide-conjugated pegylated phospholipid (DSPE-MaL-PEG2000) were prepared by probe sonication. Fluorescent probes were incorporated into liposomes for biophysical and/or biochemical analysis and/or triggered-release assays. Affibody was conjugated to these liposomes via its C-terminal cysteine by incubation in the presence of a reducing agent (e.g., tributylphosphine) for 16-20 hours under an argon atmosphere. Lipid-conjugated affibody molecule was visible as an 11.3-kDa band on a 4-12% Bis/Tris gel under reducing conditions. Affibody conjugation yields were approximately 70% at a protein-lipid ratio of 20 microg/mg, with an average number of 200 affibody molecules per Affisome. Affibody conjugation to thermosensitive liposomes did not have any significant effect on the hydrodynamic size distribution of the liposomes. Thermosensitivity of Affisomes was determined by monitoring the release of entrapped calcein (a water-soluble fluorescent probe, lambdaex/em 490/515 nm) as a function of temperature. Calcein was released from Affisomes (thermosensitive liposomes with affibody-Targeted SUV) as well as nontargeted SUV (thermosensitive liposomes without affibody) in a temperature-dependent manner, with optimal leakage (90-100%) at 41 degrees C. In contrast, liposomes prepared from Egg phosphatidyl choline (Egg PC, Tm approximately 0 degrees C) under similar conditions released only 5-10% calcein at 41 degrees C. Affisomes, when stored at room temperature, retained > 90% entrapped calcein up to 7 days. Moreover, incubation of liposomes in phosphate-buffered saline, supplemented with 10% heat-inactivated serum (fetal bovine serum) did not result in a destabilization of liposomes. Therefore, Affisomes present promising, novel drug-delivery candidates for breast cancer targeting.
The Application of Programmable DSPs in Mobile Communications (2001)

From the Publisher:
Digital Signal Processors (DSPs) are unique microprocessors that are programmable and operate in real time - much faster than general-purpose microprocessors. They are particularly well suited to handling the demands of processing information and are used in 70% of the world's digital cellular phones. The book brings together the broad, rapidly expanding field of DSP based applications for the mobile communications market, providing coverage many applications, such as the modem application for cellular communications, as well as user interface applications such as speech, video, security, recognition, etc. It will also include a discussion of the historical and future developments of DSPs, giving the reader a perspective on why DSPs are used in mobile communications and the challenges to their future use. First book to cover DSP based applications in mobile communications Includes many applications of DSPs, including speech, video, security, recognition Covers the historical and future development of DSPs for these applications Authored by the industry leaders in DSP based applications A valuable, leading edge resource for engineers and researchers working in wireless cellular communications.
The signaling pathway mediated by the type IIB activin receptor controls axial patterning and lateral asymmetry in the mouse. (1997)

Vertebrate animals exhibit segmented axial skeletons and lateral asymmetry of the visceral organs. The segment identity of individual vertebrae is believed to be determined by a combination of functionally active Hox genes that have defined expression boundaries along the anteroposterior axis (known as the axial Hox code). Disturbance of the Hox code by ectopic expression or mutation of Hox genes often leads to homeotic transformation of the vertebrae. Largely unknown, however, are the signaling molecules that provide the positional cues for the precise establishment and maintenance of the Hox code. In this study we show that disruption of the type IIB activin receptor (ActRIIB) by gene targeting results in altered expression of multiple Hox genes and abnormal patterning of the vertebrae, similar to but severer than retinoic acid (RA)-induced anterior transformation. We further show that RA and ActRIIB mutation have synergistic effects on vertebral patterning. Activin, Vg-1 and, type II activin receptors have been implicated in regulation of lateral asymmetry during chick and Xenopus development. We show here that the ActRIIB-/- mice die after birth with complicated cardiac defects including randomized heart position, malposition of the great arteries, and ventricular and atrial septal defects. In addition, the heart anomalies are associated with right pulmonary isomerism and splenic abnormalities, recapitulating the clinical symptoms of the human asplenia syndrome. These findings provide genetic evidence that the ActRIIB-mediated signaling pathway plays a critical role in patterning both anteroposterior and left-right axes in vertebrate animals.
DNA Repair Defect Tied to Mutated TGF-β Receptor Gene (1995)

Article MetricsDownloadsCitationsNo data available.01234AugSepOctNovDecJan240Total6 Months12 MonthsTotal number of downloads for the most recent 6 whole calendar months.
An oriented, siliceous deca-dodecasil 3R (DDR) zeolite film for effective carbon capture: insight into its hydrophobic effect (2017)

An oriented, hydrophobic membrane made of a decadodecasil 3 rhombohedral (DDR) zeolite (0.36 nm) could separate CO<sub>2</sub>(0.33 nm) from larger N<sub>2</sub>(0.364 nm) reliably.