gebhart/patrepeval huggingface dataset:
Interaction between G-protein beta and gamma subunit types is selective. (1992)

Signal-transducing guanine nucleotide-binding proteins (G proteins) are made up of three subunits, alpha, beta, and gamma. Each of these subunits comprises a family of proteins. The rules for association between members of one family with members of another to form a multimer are not known; it is not clear whether associations are specific or nonspecific. Other than transducin (Gt), the G protein in rod photoreceptors, most purified G proteins contain more than one subtype of beta or gamma subunits. The Gt alpha subunit is associated only with beta 1 and gamma 1. It is not known whether this specificity is due to the differential expression of these subunit types in a cell type or due to intrinsically different affinities between different beta and gamma subunit types. We have used a transfected cell assay system to examine the association of the beta 1, beta 2, and beta 3 proteins with the gamma 1 and gamma 2 proteins. Results show that gamma 1 does not associate with beta 2 and that beta 3 does not associate with gamma 1 or gamma 2. Differences in affinities between types of G protein subunits will impose restrictions on the formation of certain heterotrimers and determine which G protein will be active in a cell. A chimeric molecule of beta 1 and beta 2 was used to broadly map the regions on these subunits that determine specificity of association.
Characterization of the p-Coumaric Acid Decarboxylase from Lactobacillus plantarum CECT 748T (2008)

It was previously reported that cell cultures from Lactobacillus plantarum CECT 748 (T) were able to decarboxylate phenolic acids, such as p-coumaric, m-coumaric, caffeic, ferulic, gallic, and protocatechuic acid. The p-coumaric acid decarboxylase (PDC) from this strain has been overexpressed and purified. This PDC differs at its C-terminal end when compared to the previously reported PDC from L. plantarum LPCHL2. Because the C-terminal region of PDC is involved in enzymatic activity, especially in substrate activity, it was decided to biochemically characterize the PDC from L. plantarum CECT 748 (T). Contrarily to L. plantarum LPCHL2 PDC, the recombinant PDC from L. plantarum CECT 748 (T) is a heat-labile enzyme, showing optimal activity at 22 degrees C. This PDC is able to decarboxylate exclusively the hydroxycinnamic acids p-coumaric, caffeic, and ferulic acids. Kinetic analysis showed that the enzyme has a 14-fold higher K(M) value for p-coumaric and caffeic acids than for ferulic acid. PDC catalyzes the formation of the corresponding 4-vinyl derivatives (vinylphenol and vinylguaiacol) from p-coumaric and ferulic acids, respectively, which are valuable food additives that have been approved as flavoring agents. The biochemical characteristics showed by L. plantarum PDC should be taken into account for its potential use in the food-processing industry.
Induced Fit Selection of a Barbiturate Receptor from a Dynamic Structural and Conformational/Configurational Library (1999)

The selection of the receptor presenting the strongest affinity for a barbiturate substrate from a dynamic combinatorial library of constituents differing in structure and conformation/configuration is described. The gradual addition of the barbiturate to an equilibrating mixture of hydrazone isomers leads to the quantitative shift towards a single species, 3, which presents highest complementarity to the substrate and yields the supramolecular entity 3:4.
A Covalent Lock for Self-Assembled Oligonucleotide Conjugates (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Covalent Lock for Self-Assembled Oligonucleotide ConjugatesMathias K. Herrlein, Jeffrey S. Nelson, and Robert L. LetsingerCite this: J. Am. Chem. Soc. 1995, 117, 40, 10151–10152Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/ja00145a042https://doi.org/10.1021/ja00145a042research-articleACS PublicationsRequest reuse permissionsArticle Views633Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Silane-Grafted / Moisture-Curable Ethylene—Propylene Elastomers for the Cable Industry (1986)

Abstract The present study has shown the capability of VTMOS grafting and moisture crosslinking of EP Elastomers. These capabilities are very large and have been shown to be dependent on various parameters. In EP copolymers, the crosslinking potential appears to be a function of the ethylene content, molecular weight, and molecular weight distribution. In the case of diene containing terpolymers, the process is shown to be limited by the diene content of the polymer through probable parasitic condensation reactions of grafted alkoxy silane. The good performance of the EP elastomers in silane-grafting/moisture-crosslinking can be also utilized in the modification of LDPE when crosslink rate improvement (elimination of hot-water treatment/hot-water treatment time reduction) or higher flexibility and lower shrinkage are needed. The positive results of this study made possible the development of EP elastomer compounds using silane-grafting and moisture-crosslinking for electrical applications. In Table IX and Table X are reported two EP elastomer compounds respectively for low-voltage and medium-voltage cable insulation applications. The key features of the above mentioned two EP elastomer compounds are the following: • high flexibility (comparable to conventional peroxide cure compound) • good mechanical properties • very fast extrusion (for productivity) • easy/versatile processing • high crosslinking state (set properties) • crosslinkable in ambient conditions.
The gene for the major cuticular wax‐associated protein and three homologous genes from broccoli (Brassica oleracea) and their expression patterns (1995)

The gene for the major protein (WAX9) found in surface wax of broccoli, designated wax9D, and three homologous genes (wax9A, B and C) were isolated from a genomic library using the previously isolated cDNA encoding the WAX9 protein as the probe; all four genes were sequenced. Genomic Southern blot analysis using the WAX9 cDNA as a probe showed the presence of at least four homologous genes in broccoli genome. The sequence of the originally isolated WAX9 cDNA matched with that of gene D. All four genes have an intron two codons before the stop codon. The putative promoter regions of the four genes, beyond the first 200 bp immediately 5' to the translation start sites, are quite different. Essential elements such as TATA and CAAT boxes and several regions homologous to the promoter regions of other plant Itp genes were identified. The expression patterns of the genes were determined by RT-PCR with gene-specific primers and sequencing of the PCR products. All the genes were expressed in leaves and flower buds. While genes A, B and D also were expressed in stems and open flowers, expression of gene C was not detected in these organs. None of them were expressed in roots. The 972 bp 5'-flanking region of wax9D when fused to beta-glucuronidase (GUS) gene, directed tissue-specific GUS expression in transgenic tobacco plants; GUS expression was found in the epidermis of leaves, stems and flower petals, sepals, trichomes, and ovules but not in roots.
Structure of the Human CALM1 Calmodulin Gene and Identification of two CALM1 ‐Related Pseudogenes CALM1P1 and CALM1P2 (1994)

The human CALM1 calmodulin gene has been isolated and characterized. The gene contains six exons spread over about 10 kb of genomic DNA. The exon-intron structure is identical to that of the human CALM3 and of the rat CALM1 and CALM3 genes. A cluster of transcription-start sites was identified 200 bp upstream of the ATG translation-start codon, and several putative regulatory elements were found in the 5' flanking region as well as in intron 1. Sequence comparison with the rat CALM1 gene revealed significant similarities in the promoter regions of the two genes and an even more striking degree of identity (70%) in the available intron 1 sequences. A short CAG trinucleotide repeat region was identified in the 5' untranslated region of the human CALM1 gene; this sequence is not conserved in the rat counterpart. Expression of the CALM1 gene was detected in all human tissues tested, although at varying levels. A 1.7-kb mRNA was uniformly present at comparable levels, whereas a 4.2-kb mRNA species was particularly abundant in brain and skeletal muscle. Clones for two different CALM1-related pseudogenes CALM1P1 and CALM1P2 were also isolated and characterized. Both pseudogenes are intronless and non-functional as judged from the presence of mutations abolishing the open reading frame. Genomic Southern analysis indicates that the human CALM1 gene/pseudogene subfamily comprises at least three but probably no more than four members. The entire family consists of three bona fide CALM genes, at least one expressed calmodulin-like CALML gene as well as at least five pseudogenes.
Basic antiinflammatory compounds. N,N',N''-Trisubstituted guanidines (1980)

A variety of basic N,N',N'',-trisubstituted guanidines was prepared and tested for antiinflammatory activity. Compounds with a thiazolylguanidine moiety linked to the 4 position of the 2-methylquinoline ring exhibited fairly high antiinflammatory activity. Optimal activity was associated with the presence of N-cycloalkyl substituents on N''-4-(2-methylquinolyl)-N'-2-thiazolylguanidine. Pharmacological data on N-cyclohexyl-N''-4-(2-methylquinolyl)-N'-2-thiazolylguanidine (SR 1368, 44) are presented and discussed.
Contact urticaria from diethyl fumarate (1985)

The contact urticariagenic properties of diethyl fumarate were studied using the guinea pig ear swelling assay and by open application on the human upper back skin. Diethyl fumarate caused non-immunologic contact urticaria in both human and guinea pig skin, and the reactions exhibited similar dose dependency and timing of maximal response.
Ber-EP4 immunoreactivity in normal skin and cutaneous neoplasms. (1995)

Ber-EP4 is an antibody raised against a cell membrane glycoprotein of, as yet, unknown function. In the skin, the ability to distinguish basal cell carcinoma from squamous cell carcinoma has been emphasized. Immunoreactivity in apocrine and eccrine secretory coil epithelium, Merkel cell carcinoma, and cutaneous mixed tumor has been reported. Having observed more widespread staining than previously reported, we sought to characterize further Ber-EP4 immunoreactivity by examining 76 formalin-fixed and paraffin-embedded proliferative epithelial skin lesions, 2 sections of fetal skin with developing adnexa, and frozen sections of skin from Mohs surgery procedures, using hematoxylin and eosin and Ber-EP4 immunostaining. In paraffin-embedded skin, matrical and outer sheath epithelium of vellus anagen follicles, inferior segment epithelium of vellus telogen follicles, and secretory coils of sweat glands stain with Ber-EP4, but anagen terminal follicle epithelium and eccrine and apocrine sweat ducts are negative. On frozen section, additional staining of eccrine ducts and cells in the outer sheath and matrix of the inferior segment of terminal follicles is noted. Basaloid epithelium of trichoepitheliomas, follicular induction over dermatofibromas, mammary Paget's disease, and selected other neoplasms showing eccrine or apocrine differentiation stain with Ber-EP4. These data indicate that in addition to basal cell carcinoma, Ber-EP4 staining may be seen in a variety of proliferative epidermal and adnexal lesions.
Aniline: A Catalyst for Sialic Acid Detection (2009)

Acid test: Sialic acid-containing glycoconjugates can be specifically and covalently tagged by mild oxidation followed by treatment with aminooxy reagents, but the method has proven impractical for many biological samples. A new report demonstrates that aniline catalysis provides a striking improvement in sialic acid detection that promises to catalyze future glycomics studies.
Trafficking of lysosomal enzymes 1 (1987)

The targeting of lysosomal enzymes from their site of synthesis in the rough endoplasmic reticulum (RER) to their final destination in lysosomes is directed by a series of protein and carbohydrate recognition signals on the enzymes. Lysosomal enzymes, along with secretory and plasma membrane proteins, contain amino-terminal signal sequences that direct the vectorial discharge of the nascent proteins into the lumen of the RER. The three classes of proteins also share a common peptide signal for asparagine glycosylation. The next signal is unique to lysosomal enzymes and permits their high-affinity binding to a specific phosphotransferase that catalyzes the formation of the mannose 6-phosphate recognition marker. This carbohydrate determinant allows binding to specific receptors that translocate the lysosomal enzymes from the Golgi complex to an acidified prelysosomal compartment. There the lysosomal enzymes are discharged for final packaging into lysosomes. Two distinct mannose 6-phosphate receptors have been identified, and cDNAs encoding their entire sequences have been cloned. An analysis of the deduced amino acid sequences of the receptors shows that each is composed of four structural domains: a signal sequence, an extracytoplasmic amino-terminal domain, a hydrophobic membrane-spanning region, and a cytoplasmic domain. The entire extracytoplasmic region of the small receptor is homologous to the 15 repeating domains that constitute the extracytoplasmic portion of the large receptor.
Kyotorphin (tyrosine-arginine) synthetase in rat brain synaptosomes. (1987)

Kyotorphin (Tyr-Arg) is a unique neuropeptide which produces analgesia by releasing Met-enkephalin from slices of the brain and spinal cord. Recent studies revealed that kyotorphin possesses the properties of neurotransmitter/neuroregulator. In the present study, we identified a kyotorphin synthetase in the soluble fraction of rat brain synaptosomes (synaptosol) and characterized it. The enzyme partially purified with Sephacryl S-300 showed an absolute requirement for ATP, MgCl2, tyrosine, and arginine. The optimal pH was 7.5-9.0 and the pI was determined to be 6.1-6.2 by isoelectric focusing. The Km was 25.6 microM for tyrosine, 926 microM for arginine, 294 microM for ATP, and 442 microM for MgCl2. The Vmax was 34.0 pmol/mg of protein/h. The apparent molecular size of this "kyotorphin synthetase" further purified by the DE52 column was 240,000-245,000 daltons, estimated using TSKgel G4000SW column chromatography. The enzyme reaction is represented by the following equation: Tyr + Arg + ATP + MgCl2 + kyotorphin synthetase----Tyr-Arg (kyotorphin) + AMP + PPi + MgCl2 + kyotorphin synthetase. The regional distribution and subcellular localization of the synthetase showed a close correlation to that of kyotorphin levels in the rat brain. The amounts of kyotorphin formed from amino acids by the synthetase in the dialyzed synaptosol was 3.0-4.0 times higher than that from precursor proteins by processing enzymes within the 30 min incubation.
Chemical vapor deposition of thin polymer films used in polymer‐based light emitting diodes (1997)

A chemical vapor deposition (CVD) technique for the preparation of the conjugated polymer PPV , poly(phenylenevinylene), is presented. The thin films were prepared by CVD of a chlorine precursor polymer from a dichloro‐ p ‐xylene monomer followed by a thermal conversion step. The preparation and apparatus are detailed and results of UV‐vis and IR spectroscopy discussed. Spontaneous orientation of the polymer during growth is revealed, which suggests the possibility of fabricating chain‐oriented polymers by CVD.
Anaerobic Induction of Alanine Aminotransferase in Barley Root Tissue (1989)

Alanine aminotransferase, otherwise called glutamate-pyruvate aminotransferase (GPT), activity increases up to fourfold during several days of anaerobic induction in barley (Hordeum vulgare L.) roots, reaching a maximum activity of 13 international units per gram fresh weight. This increase in activity paralleled the increase in alcohol dehydrogenase activity in the same root tissue. Upon return to aerobic conditions, the induced GPT activity declined with an apparent half-life of 2 days. The isozyme profile of GPT in barley root tissue comprised one band of activity; in maize there were three bands of activity, the bands with greater mobility had much lower activity. Native polyacrylamide gel electrophoresis indicated that the induction of GPT activity results from an increase in the level of activity of these bands; no other activities were detected. When root tissue was induced under different levels of hypoxia (0%, 2%, 5%, and 21% O(2)), changes in GPT activity were found to increase with lower levels of oxygen. Comparisons of GPT induction in barley, maize (Zea mays), rye, (Secale cereale) and wheat (Triticum aestivum) indicate that this enzyme is induced in the root tissue of all of these cereals; however, anaerobic root conditions do not result in the induction of GPT activity in leaf tissue. The dependence of GPT induction on high levels of nitrate in the media was tested by comparing activity levels in Hoagland solution and a nitrate-free nutrient solution. GPT activity was induced to similar levels under both conditions. These results indicate that alanine aminotransferase shows a very similar pattern of induction to alcohol dehydrogenase in barley root tissue and may be important in anaerobic glycolysis.
Alveolar macrophages lack CCR2 expression and do not migrate to CCL2 (2007)

These data support the specificity of CCL2 to selectively drive monocyte, but not alveolar macrophage recruitment to the lung and CCR5 as the primary macrophage receptor for CCL3.
Ecological chemistry and biochemistry of plant terpenoids (1991)

Recent chemistry of conifer terpenoids, A. San Feliciano and J.L. Lopez terpenoids of marine plants, S. De Rosa chemistry, biogenesis, and chemotaxonomy of the diterpenoids of Salvia, J.G. Luis environmental control of essential oil production in Mediterranean plants, J.D. Ross and C. Sombrero terpenoid production in plant cell cultures, B.V. Charlwood and K.A. Charlwood plant iridoids, their biosynthesis and distribution in angiosperms, S.R. Jensen terpenoid phytoalexins - aspects of biosynthesis, catabolism, and regulation, D.R. Threlfall and I.M. Whitehead inhibition of phytosterol biosyntesis and the consequences for plant growth, L.J. Goad pharmacology of diterpenoids, M.J. Alcaraz and J.L. Rios plant saponins - chemistry and molluscicidal action, A. Marston and K. Hostettmann chemical ecology of terpenoid and other frangrances of angiosperm flowers, G. Bergstrom lower terpenoids as natural insect control agents, J.A. Pickett sequestration of iridoids by insects, H. Rimpler plant ecdysteroids and their interaction with insects, F. Camps plants terpenoids as allelopathic agents, N.H. Fisher recent advances in the ecological chemistry of plant terpenoids, J.B. Harborne.
Radioiodinated fatty acids for myocardial imagine: effects on chain length. (1981)

Terminally iodinated long-chain fatty acids have been used experimentally and clinically as myocardial imaging agents. Six omega-iodo fatty acids (I(CH2)n CO2H, where n = 10, 12, 15, 18, 21, 26) have been synthesized and tested in rats. Myocardial extraction values and heart-to-blood ratios are affected by chain length. Extraction is shown to be highest for n = 18 and 21, as are heart-to-blood ratios at 5 min. The cellular fate of the fatty acid changes form that of beta -oxidation for n less than or equal to 15 to predominantly triglyceride storage for n = 18 and 21, as shown by analysis of rat heart homogenates by thin layer chromatography at two time intervals.
Immunoelectron microscopic localization of transforming growth factor beta 1 and latent transforming growth factor beta 1 binding protein in human gastrointestinal carcinomas: qualitative difference between cancer cells and stromal cells. (1993)

Transforming growth factor beta 1 (TGF-beta 1) is secreted as an inactive complex associated with latent TGF-beta 1 binding protein (LTBP). Tissue localization of these proteins has not been fully understood in human pathological conditions. We examined the immunohistochemical localization of TGF-beta 1 precursor (proTGF-beta 1) and LTBP in carcinomas and granulation tissue in the human gastrointestinal tract at the light and electron microscopic levels. In normal tissue, endothelial cells and granulocytes sporadically showed immunoreactivity for proTGF-beta 1, while epithelial cells were all negative. In cancer tissue, both cancer cells and stromal cells (fibroblasts, macrophages, and endothelial cells) were positive for proTGF-beta 1, more frequently in diffuse-type gastric carcinomas than in differentiated-type adenocarcinomas. Immunoelectron microscopy revealed that proTGF-beta 1 was localized in rough endoplasmic reticulum and perinuclear cisternae in fibroblasts, macrophages, and endothelial cells in cancer stroma and in fibrous granulation tissue. In contrast, the intracellular localization of proTGF-beta 1 in carcinoma cells was predominantly observed in the cytosol (cytoplasmic matrix). This finding suggests disarranged or blocked intracellular transportation of proTGF-beta 1 in cancer cells. The immunoreactivity for LTBP was not observed in the normal epithelial cells. It was localized in cancer stroma, not in cancer cells. Ultrastructurally, LTBP was located in the extracellular matrix around fibroblasts and smooth muscle cells. The intracellular immunoreactivity for LTBP was observed in rough endoplasmic reticulum of fibroblasts and smooth muscle cells, the same as in granulation tissue. These results suggest that gastrointestinal carcinoma cells produce no or a small amount of LTBP in vivo. Our investigation suggests that extensive fibrosis in both cancer stroma and granulation tissues may be promoted by TGF-beta 1 mainly secreted from stromal cells.
Laser abrasion for cosmetic and medical treatment of facial actinic damage. (1989)

Previous studies have shown the carbon dioxide (CO2) laser to be effective in the treatment of actinic cheilitis. After CO2 laser abrasion, normal skin and marked cosmetic improvement of the lip were noted. In our study, twenty-three patients were treated with CO2 laser abrasions for cosmetic improvement of facial lines and actinic changes. Pre- and postoperative histopathologic examinations were made on two patients. Preoperative examination of specimens from actinically damaged skin showed atypical keratinocytes in the basal layer of the epidermis, with overlying dense compact orthokeratosis and parakeratosis. Abundant solar elastosis was seen in the papillary dermis. Postoperative histologic specimens showed a normal-appearing epidermis with fibrosis in the papillary dermis and minimal solar elastosis (about four weeks after laser treatment). At present, various modalities are available for the regeneration of the aged skin, including chemical peels and dermabrasion. Significantly fewer complications were noted with CO2 laser abrasion than with these methods. Thus, CO2 laser abrasion can be useful in the cosmetic and medical treatment of the aged skin. Marked clinical and histologic improvement has been demonstrated.