gebhart/patrepeval huggingface dataset:
Efficient Crosspolar Optimization of Shaped-Beam Dual-Polarized Reflectarrays Using Full- Wave Analysis for the Antenna Element Characterization (2016)

A method for the optimization of the crosspolar component of dual-polarized reflectarrays using full-wave analysis at the element level is described and demonstrated. The reflectarray full-wave analysis is based on local periodicity (LP) and integrated within the optimization process in order to accurately characterize the crosspolar far field. The proposed method is based on the generalized Intersection Approach framework using the Levenberg-Marquardt Algorithm as backward projector, and the employed full-wave analysis is based on the Method of Moments assuming Local Periodicity (MoM-LP). Several strategies to accelerate the computations are exploited, such as the parallelization of all the algorithm building blocks. To minimize the impact of MoM-LP in the optimization process, a strategy to reduce the number of MoM-LP calls is described, further accelerating the algorithm. Moreover, the convergence is improved by working with the squared field amplitude, alleviating the trap problem of local optimizers. This method allows to optimize the crosspolar component in the whole visible region or only in the coverage zone to facilitate the convergence, reducing computing time and memory usage. Two test cases are provided to validate the technique, one with an isoflux pattern for global Earth coverage and another with European coverage for direct broadcast satellite application.
Hydrogen as a Cause of Doping in Zinc Oxide (2000)

Zinc oxide, a wide-band-gap semiconductor with many technological applications, typically exhibits n-type conductivity. The cause of this conductivity has been widely debated. A first-principles investigation, based on density functional theory, produces strong evidence that hydrogen acts as a source of conductivity: it can incorporate in high concentrations and behaves as a shallow donor. This behavior is unexpected and very different from hydrogen's role in other semiconductors, in which it acts only as a compensating center and always counteracts the prevailing conductivity. These insights have important consequences for control and utilization of hydrogen in oxides in general.
Extracellular ATP Activates Transcription Factor NF-κB through the P2Z Purinoreceptor by Selectively Targeting NF-κB p65 (RelA) (1997)

Cells of the macrophage lineage express a peculiar surface receptor for extracellular ATP, designated P2Z/P2X7 purinergic receptor, that induces pore formation and collapse of the plasma membrane potential. Although the function of the P2Z receptor is largely unknown, accumulating evidence implicates its role in cell signaling and immune reactions. Here, we investigated the effect of P2Z receptor ligation on the activation of NF-kappaB, a transcription factor controlling cytokine expression and apoptosis. Exposure of microglial cells to ATP but not other nucleotides resulted in potent NF-kappaB activation. This effect was specifically mediated by the P2Z receptor, because selective receptor antagonists prevented NF-kappaB activation. NF-kappaB activation required reactive oxygen intermediates and proteases of the caspase family, because it was abolished by antioxidants and specific protease inhibitors. The subunit composition of the ATP-induced NF- kappaB-DNA complex was rather unusual. Whereas exposure to LPS-induced prototypical NF-kappaB p50 homo- and p65 (RelA)/p50 heterodimers, ATP stimulation resulted in the sole appearance of a p65 homodimer. This is the first demonstration that a certain stimulus activates a particular NF-kappaB subunit. Because different NF-kappaB complexes exhibit distinct transcriptional and DNA-binding activities, ATP may control the expression of a subset of NF-kappaB target genes distinct from those activated by classical proinflammatory mediators.
Reprogramming of adult rod photoreceptors prevents retinal degeneration (2013)

A prime goal of regenerative medicine is to direct cell fates in a therapeutically useful manner. Retinitis pigmentosa is one of the most common degenerative diseases of the eye and is associated with early rod photoreceptor death followed by secondary cone degeneration. We hypothesized that converting adult rods into cones, via knockdown of the rod photoreceptor determinant Nrl, could make the cells resistant to the effects of mutations in rod-specific genes, thereby preventing secondary cone loss. To test this idea, we engineered a tamoxifen-inducible allele of Nrl to acutely inactivate the gene in adult rods. This manipulation resulted in reprogramming of rods into cells with a variety of cone-like molecular, histologic, and functional properties. Moreover, reprogramming of adult rods achieved cellular and functional rescue of retinal degeneration in a mouse model of retinitis pigmentosa. These findings suggest that elimination of Nrl in adult rods may represent a unique therapy for retinal degeneration.
NGF and BDNF signaling control amyloidogenic route and Aβ production in hippocampal neurons (2008)

Here, we report that interruption of NGF or BDNF signaling in hippocampal neurons rapidly activates the amyloidogenic pathway and causes neuronal apoptotic death. These events are associated with an early intracellular accumulation of PS1 N-terminal catalytic subunits and of APP C-terminal fragments and a progressive accumulation of intra- and extracellular Abeta aggregates partly released into the culture medium. The released pool of Abeta induces an increase of APP and PS1 holoprotein levels, creating a feed-forward toxic loop that might also cause the death of healthy neurons. These events are mimicked by exogenously added Abeta and are prevented by exposure to beta- and gamma-secretase inhibitors and by antibodies directed against Abeta peptides. The same cultured neurons deprived of serum die, but APP and PS1 overexpression does not occur, Abeta production is undetectable, and cell death is not inhibited by anti-Abeta antibodies, suggesting that hippocampal amyloidogenesis is not a simple consequence of an apoptotic trigger but is due to interruption of neurotrophic signaling.
Implementing and configuring SAP event management (EM) (2010)

The influence of pretreatment natural killer (NK) cell activity on patterns of disease recurrence in patients with head and neck cancer was studied. One hundred eighty-three patients were followed prospectively for disease recurrence following definitive therapy (median disease-free follow-up = 15 months). Pretreatment peripheral blood NK cell activity against K562 target cells was measured for each patient and expressed in lytic units (LU). The risk of death caused by recurrent disease was greatest in those patients with peripheral blood NK cell activity less than 60 LU (P less than 0.05). The site of recurrence could also be distinguished by LU value. As a percentage of total recurrences, locally recurrent disease was predominant in the patients with NK function greater than 60 LU whereas distant metastases predominated in the group of patients with peripheral blood NK cell activity less than 60 LU. These patterns were maintained when patients were stratified by clinical N stage (N = 0 versus N+), T stage (T-1 or T-2 versus T-3 or T-4), and treatment regimen (surgery alone versus surgery and postoperative radiation therapy). Natural killer cell activity appears to have prognostic significance, both for disease-free survival and for site of recurrence independent of clinical stage. The indications for multimodality therapy for patients with head and neck cancer when stratified by NK cell function need to be clarified.
A prosurvival DNA damage-induced cytoplasmic interferon response is mediated by end resection factors and is limited by Trex1 (2017)

Radiotherapy and chemotherapy are effective treatment methods for many types of cancer, but resistance is common. Recent findings indicate that antiviral type I interferon (IFN) signaling is induced by these treatments. However, the underlying mechanisms still need to be elucidated. Expression of a set of IFN-stimulated genes comprises an IFN-related DNA damage resistance signature (IRDS), which correlates strongly with resistance to radiotherapy and chemotherapy across different tumors. Classically, during viral infection, the presence of foreign DNA in the cytoplasm of host cells can initiate type I IFN signaling. Here, we demonstrate that DNA-damaging modalities used during cancer therapy lead to the release of ssDNA fragments from the cell nucleus into the cytosol, engaging this innate immune response. We found that the factors that control DNA end resection during double-strand break repair, including the Bloom syndrome (BLM) helicase and exonuclease 1 (EXO1), play a major role in generating these DNA fragments and that the cytoplasmic 3'-5' exonuclease Trex1 is required for their degradation. Analysis of mRNA expression profiles in breast tumors demonstrates that those with lower Trex1 and higher BLM and EXO1 expression levels are associated with poor prognosis. Targeting BLM and EXO1 could therefore represent a novel approach for circumventing the IRDS produced in response to cancer therapeutics.
Serial Echocardiographic-Doppler Assessment of Left Ventricular Geometry and Function in Rats With Pressure-Overload Hypertrophy (1995)

This model of pressure overload is characterized initially by concentric LV hypertrophy with compensated LV chamber performance; however, markedly abnormal diastolic filling is present. The transition from compensated hypertrophy to early failure is heralded by LV dilation, impairment of systolic function, and progression of the abnormalities in LV filling. Chronic ACE inhibition in rats with supravalvular aortic banding (1) does not change in vivo LV systolic pressure but prevents increased LV cavity size and increased LV wall stress and (2) attenuates impairment of (or improves) both systolic and diastolic functions. The effects of fosinopril could be explained in part by inhibition of an intracardiac renin-angiotensin system.
Direct Feedback Alignment Provides Learning in Deep Neural Networks (2016)

Artificial neural networks are most commonly trained with the back-propagation algorithm, where the gradient for learning is provided by back-propagating the error, layer by layer, from the output layer to the hidden layers. A recently discovered method called feedback-alignment shows that the weights used for propagating the error backward don't have to be symmetric with the weights used for propagation the activation forward. In fact, random feedback weights work evenly well, because the network learns how to make the feedback useful. In this work, the feedback alignment principle is used for training hidden layers more independently from the rest of the network, and from a zero initial condition. The error is propagated through fixed random feedback connections directly from the output layer to each hidden layer. This simple method is able to achieve zero training error even in convolutional networks and very deep networks, completely without error back-propagation. The method is a step towards biologically plausible machine learning because the error signal is almost local, and no symmetric or reciprocal weights are required. Experiments show that the test performance on MNIST and CIFAR is almost as good as those obtained with back-propagation for fully connected networks. If combined with dropout, the method achieves 1.45% error on the permutation invariant MNIST task.
[Antileukotriene drugs: a new treatment for asthma]. (1999)

Asthma incidence and prevalence have significantly increased during the last 25 years, making this pathology to be one of the most diffused in the industrialized world. It affects 5% of the adult population and the 10-15% of the children. The aethipathogenesis of asthma clearly involves a number of mediators, among which leukotrienes play an important role inducing bronchoconstriction, oedema and mucus hypersecretion. Thus, compounds inhibiting either the action or formation of these mediators are potentially interesting as antiasthma drugs. Two strategies for modulating the actions of the leukotrienes are currently undergoing clinical evaluation: cysteinyl leukotriene receptor antagonism and 5-lipoxygenase (5-LO) inhibition. These two classes of agents are here reviewed and their clinical utility as antiasthma drugs is evaluated.
Corn leaf volatiles: indentification using Tenax trapping for possible insect attractants (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorn leaf volatiles: indentification using Tenax trapping for possible insect attractantsRon G. Buttery and Louise C. LingCite this: J. Agric. Food Chem. 1984, 32, 5, 1104–1106Publication Date (Print):September 1, 1984Publication History Published online1 May 2002Published inissue 1 September 1984https://pubs.acs.org/doi/10.1021/jf00125a044https://doi.org/10.1021/jf00125a044research-articleACS PublicationsRequest reuse permissionsArticle Views404Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A large fraction of HLA class I ligands are proteasome-generated spliced peptides (2016)

The proteasome generates the epitopes presented on human leukocyte antigen (HLA) class I molecules that elicit CD8<sup>+</sup> T cell responses. Reports of proteasome-generated spliced epitopes exist, but they have been regarded as rare events. Here, however, we show that the proteasome-generated spliced peptide pool accounts for one-third of the entire HLA class I immunopeptidome in terms of diversity and one-fourth in terms of abundance. This pool also represents a unique set of antigens, possessing particular and distinguishing features. We validated this observation using a range of complementary experimental and bioinformatics approaches, as well as multiple cell types. The widespread appearance and abundance of proteasome-catalyzed peptide splicing events has implications for immunobiology and autoimmunity theories and may provide a previously untapped source of epitopes for use in vaccines and cancer immunotherapy.
Franklin’s disease: Ig gamma 2 H chain mutant BUR (1992)

The complete sequence of a gamma 2-H chain disease protein BUR is presented. This mutant, a dimer of a 348-residue chain, linked by four disulfide bridges, is composed of a complete V region, hinge, CH2, and CH3 domains. There is one deletion, the CH1 domain, which includes the cysteine residue bridging the H to L chain. Although the V region is encoded by the VHI and JHIII genes, it has several distinctions: methionine at position 11, two unique cysteine residues in the second complementarity determining region (CDR2), and three glycosylation sites, two of which are located in the CDR2 and CDR3 regions. These distinctive characteristics of BUR VH within the framework of a normal VHI may be affected by extensive somatic mutation or by a rare and previously unanalyzed VH gene.
Mitotic and G 2 Checkpoint Control: Regulation of 14-3-3 Protein Binding by Phosphorylation of Cdc25C on Serine-216 (1997)

Human Cdc25C is a dual-specificity protein phosphatase that controls entry into mitosis by dephosphorylating the protein kinase Cdc2. Throughout interphase, but not in mitosis, Cdc25C was phosphorylated on serine-216 and bound to members of the highly conserved and ubiquitously expressed family of 14-3-3 proteins. A mutation preventing phosphorylation of serine-216 abrogated 14-3-3 binding. Conditional overexpression of this mutant perturbed mitotic timing and allowed cells to escape the G2 checkpoint arrest induced by either unreplicated DNA or radiation-induced damage. Chk1, a fission yeast kinase involved in the DNA damage checkpoint response, phosphorylated Cdc25C in vitro on serine-216. These results indicate that serine-216 phosphorylation and 14-3-3 binding negatively regulate Cdc25C and identify Cdc25C as a potential target of checkpoint control in human cells.
Characterization method for a new diffusion mixer applicable in micro flow injection analysis systems (1999)

A new mixer is designed for mixing a phenolic solution into water. The mixer design is such that it can be easily adjusted for the controlled mixing of a specific compound within a certain time. This paper describes the working principle of the mixer as well as a suitable characterization method for the mixer. Measurement results are presented which show the correct working of the mixer. A quantitative measure is introduced to express the extent of mixing performed by the mixer. The characterization method allows the measurement of the flow rate, pressure drop and extent of mixing.
Cell electropermeabilization to small molecules in vitro: control by pulse parameters (2001)

A systematic study concerning the role of the different electric field parameters (pulse number, duration and amplitude) on electropermeabilization of DC3F cells to small molecules (propidium iodide) and on cell viability is presented. Cell permeabilization and viability dependence on the pulse amplitude was determined by twenty different sets of electrical parameters. The number of pulses varied between 1 and 64 and pulse duration between 20 ms and 1 ms. The most important parameter was the pulse amplitude because it triggered the electropermeabilization process and the process of cell death. Either in the case of electropermeabilization as well as in the case of cell viability experiments, the parameter U50 (the pulse amplitude leading to permeabilization or to the death of 50% of cell population) was not changed if the set of electrical parameters consisted of more than 16 pulses. This was independent of the pulse duration. The efficiency of permeabilization was enhanced by using of longer pulses. Such a systematic study of the influence of different electric field parameters on electropermeabilization and cell viability may serve as a base for optimization of the electropermeabilization conditions for different applications.
Aerosol vaccination of mice with a live, temperature- sensitive recombinant influenza virus (1976)

Mice were vaccinated intranasally (i.n.) or with small-particle aerosols (SPA; 2 mum) or large-particle aerosols (LPA; 8 mum) of an attenuated, temperature-sensitive, recombinant A influenza (H3N2) virus, ts-1 (E). Serum virus-neutralizing and hemagglutination inhibition antibodies were detected for all vaccinated mice by 28 days. Bronchoalveolar wash fluids had increased levels of immunoglobulin (IgG, IgA) only in the i.n. -vaccinated mice. Hemagglutination and virus-neutralizing antibodies were detected in the SPA- and i.n. -vaccinated groups but not in the LPA vaccinates. Upon challenge with SPA of a mouse virulent H3N2 influenza vitus, total protection was obtained for the SPA- and I.N. -vaccinated mice, whereas only 89% of the LPA group survived. Replication of the challenge virus was signifcantly repressed in both the lower and upper respiratory tracts of the three groups of vaccinated mice compared to the nonvaccinated controls. The protection afforded the SPA- and i.n. -vaccinated mice was the same as measured for mice after recovery from earlier subelthal active infection with virulent virus.
Antinociceptive Effect of Dihydroetorphine and Its Tolerance/Dependence Liability in Mice. (1993)

The profile of actions of dihydroetorphine (DHE) concerning antinociception, tolerance and dependence was compared with those of morphine in mice. DHE at 1, 5, 10 or 20 micrograms/kg produced an antinociceptive effect in a dose dependent manner and 10 micrograms/kg was nearly equipotent to that of 10 mg/kg of morphine. The antinociceptive effect of both drugs was completely suppressed by 1 mg/kg of naloxone, while neither 10 mg/kg of naltrindole nor 1 mg/kg of nor-binaltorphimine had any suppressive effect. Mice tolerant to morphine antinociception were tolerant to DHE and vice versa. The naloxone-sensitive, locomotor accelerating activity was progressively enhanced by daily administration of DHE and morphine and a cross reverse tolerance developed between these compounds, suggesting that common mechanisms, especially mediating opioid receptors, underlay the activity enhancement. The development of physical dependence as evidenced by naloxone precipitated withdrawal signs, however, was not observed with daily treatment with DHE, 10, 20 and 100 micrograms/kg for 6 d. Thus, we demonstrated that DHE produces the antinociceptive effect mediated through mu opioid receptors without causing development of a physical dependence, suggesting that it is safe to use in the clinical therapy of patients suffering severe pain such as that accompanying cancer.
Aberrant Overexpression of Satellite Repeats in Pancreatic and Other Epithelial Cancers (2011)

Satellite repeats in heterochromatin are transcribed into noncoding RNAs that have been linked to gene silencing and maintenance of chromosomal integrity. Using digital gene expression analysis, we showed that these transcripts are greatly overexpressed in mouse and human epithelial cancers. In 8 of 10 mouse pancreatic ductal adenocarcinomas (PDACs), pericentromeric satellites accounted for a mean 12% (range 1 to 50%) of all cellular transcripts, a mean 40-fold increase over that in normal tissue. In 15 of 15 human PDACs, alpha satellite transcripts were most abundant and HSATII transcripts were highly specific for cancer. Similar patterns were observed in cancers of the lung, kidney, ovary, colon, and prostate. Derepression of satellite transcripts correlated with overexpression of the long interspersed nuclear element 1 (LINE-1) retrotransposon and with aberrant expression of neuroendocrine-associated genes proximal to LINE-1 insertions. The overexpression of satellite transcripts in cancer may reflect global alterations in heterochromatin silencing and could potentially be useful as a biomarker for cancer detection.
Programmable Magnetic Tweezers and Droplet Microfluidic Device for High‐Throughput Nanoliter Multi‐Step Assays (2012)

Tweezing out the answer: A microfluidic device combining droplets (less than 100 nL) and magnetic particles (see scheme) was implemented for fast heterogeneous multiplexed assays. Magnetic tweezers can perform the manipulations required in an immunoassay (capture, extraction, mixing, and rinsing). This method was applied to the diagnosis of congenital hypothyroidism with 14 pM sensitivity. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.