gebhart/patrepeval huggingface dataset:
Micromachining for improvement of integrated ultrasonic transducer sensitivity (1990)

A micromachined diaphragm structure for integrated ultrasonic transducers is discussed. The micromachining significantly reduces a large parasitic capacitance, resulting in improved sensitivity compared with identical transducers on solid substrates. For the particular geometry tested, sensitivity improved 8.9 dB; this result matches a prediction of 10.9 dB, based on a simple model, reasonably well. This level of improvement is considered significant for the applications under consideration (medical imaging and nondestructive evaluation).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Curative radioimmunotherapy of human mammary carcinoma xenografts with iodine-131-labeled monoclonal antibodies. (1989)

The radioiodinated monoclonal antibody BW 495/36 showed an exceptionally high uptake and long residence time in human ductal mammary carcinoma xenografts in nude mice. There was a mean tumor uptake of 82%/g 24 hr p.i., decreasing with a biologic half-life of approximately 6 days, to 15%/g by Day 16. The tumor-to-blood ratio increased from 2.8 to 21.4 and the percentage of the whole-body retention recovered in the tumor from 47% to 80% during the same time interval. The therapeutic efficiency of two injections of 7.4 MBq 131I-BW 495/36 was evaluated by comparing the tumor size with that in mice injected with either the same amount of the unlabeled MoAb, the same radioactivity of an 131I-labeled nonspecific MoAb, or with saline only. The high tumor accumulation of 131I-BW 495/36 led to a total tumor dose of 77 Gy resulting in a mean reduction in tumor diameter of 50%, corresponding to a reduction in tumor volume of 88% within 42 days p.i. Unlabeled MoAb had no effect on tumor growth compared with controls, whereas 131I nonspecific antibody caused a slight inhibition of tumor growth. Histologic tumor sections showed large areas of necrosis and a pronounced vacuolation of the tumor cell cytoplasm between Days 7 and 30 p.i. By Day 42 all remaining tissue in the tumor was identified as mouse connective tissue.
Integrative analysis of DNA copy number and gene expression in metastatic oral squamous cell carcinoma identifies genes associated with poor survival (2010)

Genes exhibiting CNA-correlated expression may have biological impact on carcinogenesis and cancer progression in OSCC. Determination of copy number-associated transcripts associated with clinical outcomes in tumor cells with an aggressive phenotype (i.e., cells metastasized to the lymph nodes) can help prioritize candidate transcripts from high-throughput data for further studies.
Energy cost of walking of amputees (1976)

A comparison of selected gait parameters and the energy cost of prosthetic walking was made in seventy patients with unilateral traumatic and vascular amputations. Amputations above the knee, below the knee, and at the Syme's level were compared in both groups of amputees, and a control group of forty normal subjects also studied. In both groups of amputees performance was significantly better the lower the level of the amputation. When preservation of function is the chief concern, amputation should be performed at the lowest possible level.
Ex ova chick chorioallantoic membrane as a novel model for evaluation of tissue responses to biomaterials and implants (2003)

One of the major obstacles in developing rationale strategies to control inflammation and fibrosis surrounding implants is the lack of a simple and inexpensive in vivo model to screen tissue reactions to various biomaterials and implants. To begin to fill this gap, we have developed an ex ova model of the chick embryo chorioallantoic membrane (CAM) for testing of tissue reaction to biomaterials and implants. For these studies, we evaluated tissue reactions (inflammation and fibrosis) to two commonly used biomaterials (nylon and silastic) grossly and histologically in the ex ova CAM. Nylon mesh was incorporated within the CAM tissue 4 days postplacement. After 8 days postplacement, the nylon mesh was totally incorporated into the CAM. Histologically, little or no inflammation was seen associated with the incorporated nylon mesh at any time point. In the case of silastic tubing, significant incorporation of the CAM was seen grossly by 1-2 days postplacement. Incorporation of the tubing continued at day 8 postplacement of the silastic tubing, with ingrowth of the CAM into the lumen of the tubing. Histological evaluation of CAMs indicated that no significant tissue reactions (inflammation or fibrosis) occurred in the CAM tissue surrounding the silastic tubing or in the CAM tissue and vasculature that had grown into the silastic tubing. To our knowledge, this report represents the first investigation of the usage of the ex ova CAM model, a shell-less chick embryo model (ex ova), as an in vivo model to test the tissue reactions to biomaterials and implants.
Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes (2006)

Selenocysteine (Sec) is cotranslationally inserted into protein in response to UGA codons and is the 21st amino acid in the genetic code. However, the means by which Sec is synthesized in eukaryotes is not known. Herein, comparative genomics and experimental analyses revealed that the mammalian Sec synthase (SecS) is the previously identified pyridoxal phosphate-containing protein known as the soluble liver antigen. SecS required selenophosphate and O-phosphoseryl-tRNA([Ser]Sec) as substrates to generate selenocysteyl-tRNA([Ser]Sec). Moreover, it was found that Sec was synthesized on the tRNA scaffold from selenide, ATP, and serine using tRNA([Ser]Sec), seryl-tRNA synthetase, O-phosphoseryl-tRNA([Ser]Sec) kinase, selenophosphate synthetase, and SecS. By identifying the pathway of Sec biosynthesis in mammals, this study not only functionally characterized SecS but also assigned the function of the O-phosphoseryl-tRNA([Ser]Sec) kinase. In addition, we found that selenophosphate synthetase 2 could synthesize monoselenophosphate in vitro but selenophosphate synthetase 1 could not. Conservation of the overall pathway of Sec biosynthesis suggests that this pathway is also active in other eukaryotes and archaea that synthesize selenoproteins.
Activation of bovine B cells via surface immunoglobulin M cross‐linking or CD40 ligation results in different B‐cell phenotypes (2000)

Experiments reported herein demonstrate that activation of bovine B cells via surface immunoglobulin M (sIgM) cross-linking, analogous to T-cell independent (TI-2) antigenic stimulation, results in the expression of CD5. Interestingly, in the presence of CD40 ligand, sIgM-mediated induction of CD5 on B cells was inhibited. These findings indicate that activation of bovine B cells via B-cell receptor (BCR) cross-linking results in a CD5+ B-cell phenotype and that CD40 signalling is inhibitory to this process. Analysis of cytokine mRNA indicates that bovine B cells constitutively express tumour necrosis factor-alpha (TNF-alpha) and interleukin (IL)-1beta transcripts in vitro, while IL-10 mRNA expression is induced following sIgM cross-linking. IL-12 p40 transcripts were produced by B cells activated by CD40, but not by BCR, ligation. Analysis of cytokine receptor mRNA indicates that activation through CD40, in the presence or absence of IgM cross-linking, results in increased IL-4 receptor-alpha (IL-4Ralpha), IL-13Ralpha1 and interferon-alpha receptor 1 (IFN-alphaR1) mRNA levels. Overall, these findings suggest that activation of bovine B cells through BCR cross-linking yields an activation phenotype that differs substantially from that of B cells activated through CD40.
Computational Studies of Epidermal Growth Factor Receptor: Docking Reliability, Three-Dimensional Quantitative Structure−Activity Relationship Analysis, and Virtual Screening Studies (2009)

An aberrant activity of the epidermal growth factor receptor (EGFR) has been shown to be related to many human cancers, such as breast and liver cancers, thus making EGFR an attractive target for antitumor drug discovery. In this study we evaluated the reliability of various kinds of docking software and procedures to predict the binding disposition of EGFR inhibitors. By application of the best procedure and use of more than 200 compounds, a receptor-based 3D-QSAR model for EGFR inhibition was developed. On the basis of the results obtained, the possibility of developing virtual screening studies was also evaluated. The VS procedure that proved to be the most reliable from a computational point of view was then used to filter the Maybridge database in order to identify new EGFR inhibitors. Enzymatic assays revealed that among the eight top-scoring compounds, seven proved to inhibit EGFR activity at a concentration of 100 microM, two of them exhibiting IC(50) values in the low micromolar range and one in the nanomolar range. These results demonstrate the validity of the methodologies followed. Furthermore, the two low micromolar compounds may be considered as very interesting leads for the development of new EGFR inhibitors.
Kinetic Study of Thermally Stimulated Dissociation of Inclusion Complex of 1-Methylcyclopropene with α-Cyclodextrin by Thermal Analysis (2008)

The thermally stimulated dissociation of the inclusion complex of 1-methylcyclopropene (1-MCP) with alpha-cyclodextrin (alpha-CD) in solid state was studied by means of thermogravimetry (TG) and differential scanning calorimetry (DSC). The mass loss of 1-MCP/alpha-CD inclusion complex occurs in four separated phases with the thermal dissociation of the inclusion complex and release of 1-MCP taking place in the second phase between 90 and 230 degrees C. The kinetic parameters of the dissociation reaction (the apparent activation energy of dissociation, E(D), the reaction order of thermal dissociation, n, and the pre-exponential factor, k0) were evaluated. The dissociation reaction was satisfactorily described by the unimolecular decay law, where the reaction order, n = 1. The effect of the molar ratio of 1-MCP to alpha-CD (inclusion ratio) in the inclusion complex on the temperature dependence of the dissociation reaction was also studied. The E(D) decreased with increasing inclusion ratio indicating higher complex stability at lower inclusion ratios. The extrapolation of the E(D) of the inclusion complexes with different inclusion ratios to 1 mol 1-MCP/mol alpha-CD yielded the "true" E(D) of 20.9 +/- 2.8 and 18.1 +/- 0.2 kJ/mol for TG and DSC, respectively. The "true" ln k(0TG) and the "true" ln k(0DSC) were also determined by extrapolation, yielding values of +4.5 +/- 1.0 and -0.3 +/- 0.3, respectively.
A comparison of chromogenic substrates for horseradish peroxidase as a label in steroid enzyme immunoassay. (1986)

Six chromogenic substrates for horseradish peroxidase (HRP) as an enzyme label were compared with regard to the sensitivity obtainable with a testosterone enzyme immunoassay system. The chromogens tested were 2, 2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), o-phenylenediamine (OPD), 5-aminosalicylic acid (5-AS), 3-amino-9-ethylcarbazole (AEC), 3, 3', 5, 5'-tetramethylbenzidine (TMB) and 3-methyl-2-benzothiazolinone hydrazone (MBTH); for comparison, a fluorimetric assay using 3-(p-hydroxyphenyl)propionic acid was also carried out. An HRP-labeled antigen was prepared by the N-succinimidyl ester method. The bound and free enzyme-labeled antigens were separated by a double antibody method. A dose-response curve with a satisfactory sensitivity was obtained in each system by the use of a minimum amount of the HRP label at an appropriate dilution of anti-testosterone antiserum (Ka=2×1010M-1). The amounts of testosterone needed to displace 50% of the bound label ranged from 9 to 150 pg. The sensitivity decreases in the order : TMB>OPD>ABTS>5-AS>MBTH>AEC. The assay using the non-mutagenic substrate, TMB, gave a high sensitivity, comparable to that of the fluorimetric method.
Cloning and characterization of aspartate-beta-semialdehyde dehydrogenase from Mycobacterium tuberculosis H37 Rv (2005)

The ASD enzyme is a validated drug target. We characterized this enzyme from M. tuberculosis and future work would focus on deducing the three-dimensional structure of the enzyme and design of inhibitors, which could be used as drugs against TB.
GENCODE reference annotation for the human and mouse genomes (2018)

The accurate identification and description of the genes in the human and mouse genomes is a fundamental requirement for high quality analysis of data informing both genome biology and clinical genomics. Over the last 15 years, the GENCODE consortium has been producing reference quality gene annotations to provide this foundational resource. The GENCODE consortium includes both experimental and computational biology groups who work together to improve and extend the GENCODE gene annotation. Specifically, we generate primary data, create bioinformatics tools and provide analysis to support the work of expert manual gene annotators and automated gene annotation pipelines. In addition, manual and computational annotation workflows use any and all publicly available data and analysis, along with the research literature to identify and characterise gene loci to the highest standard. GENCODE gene annotations are accessible via the Ensembl and UCSC Genome Browsers, the Ensembl FTP site, Ensembl Biomart, Ensembl Perl and REST APIs as well as https://www.gencodegenes.org.
Simple/rapid test devices for anti-HIV screening: Do they come up to the mark? (1999)

Thirteen simple/rapid test devices (S/RTDs) for the detection of antibodies to HIV 1 and HIV 2 were assessed. Ninety-two specimens in four categories were used and results with the thirteen S/RTDs were compared with those obtained with six currently available commercial laboratory-based anti-HIV 1/2 EIAs. Seven of the 13 S/RTDs scored all 26 blood donors' specimens as unreactive, and 11 correctly identified all the 25 "straightforward" anti-HIV positive specimens. False negative results arose when testing by Uni-Gold HIV and SeroCard HIV, which gave 72 and 68 correct positive observations, respectively, out of 75. No S/RTD detected seroconversion earlier than the most sensitive EIAs, but four S/RTDs performed similarly to most of the EIAs. On the low-titre panel specimens, six S/RTDs were less sensitive than the least sensitive EIA and, in contrast to four of the six EIAs, only one S/RTD was able correctly to identify all the positive specimens. A manufacturing problem was identified that allowed the HIV antigen-sensitised area on the membrane of two SeroCard HIV devices to be misaligned with the device's reading window so that the reaction was almost entirely obscured. As long as small numbers of specimens were involved, most S/RTDs required considerably less time and less equipment than EIAs, but overall they were slightly less sensitive. Their use in various health settings and for confirmatory procedures is discussed.
Expression of botulinum neurotoxins A and E, and associated non-toxin genes, during the transition phase and stability at high temperature: analysis by quantitative reverse transcription-PCR (2006)

Production of botulinum neurotoxin A (BoNT/A) and associated non-toxic proteins (ANTPs), which include a non-toxic non-haemagglutinin (NTNH/A) as well as haemagglutinins (HAs), was found previously to be dependent upon an RNA polymerase alternative sigma factor (BotR/A). Expression of the botR/A, bont/A and antp genes, monitored by reverse transcription and real-time PCR analysis, occurred concomitantly at the transition between the exponential and stationary growth phases of Clostridium botulinum A. The botR/A expression level was about 100-fold less than those of the bont/A and antp genes. Therefore, BotR/A is an alternative sigma factor controlling the botulinum A locus genes during the transition phase. The highest toxin concentration was released into the culture supernatant 12 h after maximum expression of the botR/A, bont/A and antp genes, without any apparent bacterial lysis. Toxin levels were then stable over 5 days in cultures at 37 degrees C, whereas a dramatic decrease in lethal activity was observed between 24 and 48 h in cultures at 44 degrees C. High temperature did inhibit transcription, since expression levels of the botR/A, bont/A and antp genes were similar in cultures at 37 and 44 degrees C. However, incubation at 44 degrees C triggered a calcium-dependent protease that degraded BoNT/A and NTNH/A, but not HAs. In C. botulinum E, which contains no gene related to botR, the bont/E and p47 genes were also expressed during the transition phase, and no protease activation at 44 degrees C was evident.
Circulating T cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 size spectratyping: correlation with immune status (1995)

The analysis of the T cell repertoires involved in local or systemic immune responses is beginning to play an important role in many clinical situations. These include autoimmunity, response to viral or bacterial superantigens, alloimmunity including allograft rejection, and tumor immunity. Here we analyze circulating T cell repertoires by determining TCR beta-chain gene complexity using a modification of V beta family-specific PCR. This approach, called CDR3 size spectratyping, uses the size heterogeneity of the CDR3 as a further source of specificity in TCR analysis. It has been used here to analyze the complexity and stability of circulating T cell repertoires in normal adults, including bone marrow donors, and bone marrow transplant recipients. Normal spectratypes are both complex and stable. The repertoire complexity of marrow recipients correlates with their state of immune function. Contractions and gaps in repertoires are revealed in individuals suffering from recurrent infections associated with T cell impairment. Spectratype analysis is applicable to other studies of specific repertoire skewing such as may be associated with immunodeficiency or found at sites of immune activity.
Droidmaker: George Lucas and the Digital Revolution (2005)

The inside story of George Lucas, his intensely private company, and their work to revolutionize filmmaking. In the process, they made computer history. Discover the birth of Pixar, digital video editing, videogame avatars, THX sound, and a host of other icons of the media age. Lucas played a central role in the universe of entertainment technologies we see every day. This book ventures in territory never explored, as Rubin--a former member of the Lucasfilm Computer Division--reconstructs the events in Hollywood, in Silicon Valley, and at Lucas' private realm in Marin County, California, to track the genesis of modern media. With unprecedented access to images and key participants from Lucasfilm, Pixar and Zoetrope--from George Lucas and the executives who ran his company, to the small team of scientists who made the technological leaps--Rubin weaves a tale of friendships, a love of movies, and the incessant forward movement of technology.--Adapted from www.droidmaker.com
Platelet-Activating Factor (PAF) Induces Corneal Neovascularization and Upregulates VEGF Expression in Endothelial Cells (2004)

PAF stimulates corneal neovascularization by a receptor-mediated mechanism. Induction of VEGF expression and stimulation of vascular endothelial cell migration are initial events in PAF-promoted corneal angiogenesis.
Arterial and venous embolization: declotting of dialysis shunts by direct injection of streptokinase. (1986)

During the past 33 months, thrombolysis of 79 clotted hemodialysis shunts was attempted by injecting small quantities of dilute streptokinase solution directly into the clotted shunt, followed by massage of the clot. Embolization of clot fragments in six of 79 cases (7.6%) was demonstrated angiographically. In four of the six cases embolization involved the brachial artery or its branches. In one case embolization involved an arm vein, and in one case embolization involved both the brachial artery and axillary vein. All patients remained asymptomatic, and repeat angiographic study, usually performed the following day, showed resolution of the emboli in four of five cases. The incidence of embolization in direct-injection thrombolysis reported in this study appears to be comparable to that reported in studies using the streptokinase infusion technique.
Prevalence of Foodborne Pathogens in Bulk Tank Milk (2001)

Bulk tank milk from 131 dairy herds in eastern South Dakota and western Minnesota was examined for the presence of for foodborne pathogens. Campylobacter jejuni, shiga-toxin producing Escherichia coli, Listeria monocytogenes, Salmonella spp., and Yersinia enterocolitica were detected in 9.2, 3.8, 4.6, 6.1, and 6.1% of bulk tank milk samples, respectively. Thirty-five of 131 (26.7%) bulk tank milk samples contained one or more species of pathogenic bacteria. Isolates of Salmonella belonged to group D (n = 4), B (n = 2), C (n = 1), and E (n = 1) "O" serogroups. All six isolates of Listeria monocytogenes were identified as O antigen type 1. Four of five isolates of E. coli encoded for the shiga-toxin 2 gene, while one strain encoded for the shiga-toxin 1 gene. Escherichia coli O157:H7 was not isolated from bulk tank milk samples. Based on autoagglutination testing, it was inferred that all eight isolates of Yersinia enterocolitica were likely to be virulent. Non A-grade (manufacturing grade) raw milk producers were at a higher risk (odd's ratio, 4.98; confidence interval, 1.96 to 12.22) of having one or more pathogens in their bulk tank milk than were Grade A producers. It was observed that 21 of 79 (26.6%) dairy producers who consumed raw milk had one or more pathogenic bacteria in their bulk tank milk. The findings of the study warrant the need for educational programs for dairy producers about the risks associated with consumption of raw milk.
Human autoantibody to RNA polymerase I transcription factor hUBF. Molecular identity of nucleolus organizer region autoantigen NOR-90 and ribosomal RNA transcription upstream binding factor. (1991)

In dividing eukaryotic cells, nucleoli disperse before mitosis and reform in daughter cells at sites of ribosomal RNA (rRNA) gene clusters that are at the secondary constrictions of chromosomes, called nucleolus organizer regions (NORs). In this study, cDNA clones for a NOR autoantigen (NOR-90) were selected using a specific human autoantibody probe and were subsequently identified to encode an alternative form of the reported human upstream binding factor (hUBF). Results from immunoprecipitation showed that anti-NOR-90 antibodies recognized both forms of hUBF/NOR-90. Our data therefore showed that UBF, a critical factor in the regulation of rRNA transcription, was tightly bound to NOR during mitosis even when rRNA synthesis was thought to be minimal. Furthermore, we identified a nucleolar transcription factor as a novel target for human autoimmune response.
Soap Dealers and Beer Barons (1982)

I've been suffering from an inferiority complex ever since reading about that 15-letter Finnish palindrome for a soap-dealer, SAIPPUAKAUPPIAS. Surely the English language can come up with something longer than the 7-letter ROTATOR, REPAPER or DEIFIED. (Yes, I know of the 9-letter MALAYALAM and the 11-letter KINNIKINNIK variant, but these are both borrowed words from non-European languages.)
28‐2: Study of the Image Blur through FFS LCD Panel Caused by Diffraction for Camera under Panel (2020)

In this paper, we analyzed image blur problems through FFS LCD panels in camera under panel (CUP) application. By careful comparison of captured images with different panel components, we find that the fully‐covered RGB photo resists and 2‐domain pixel electrodes design will be the main contributors to the diffraction‐induced problem.