gebhart/patrepeval huggingface dataset:
Mapping Urban Green Infrastructure: A Novel Landscape-Based Approach to Incorporating Land Use and Land Cover in the Mapping of Human-Dominated Systems (2018)

Common approaches to mapping green infrastructure in urbanised landscapes invariably focus on measures of land use or land cover and associated functional or physical traits. However, such one-dimensional perspectives do not accurately capture the character and complexity of the landscapes in which urban inhabitants live. The new approach presented in this paper demonstrates how open-source, high spatial and temporal resolution data with global coverage can be used to measure and represent the landscape qualities of urban environments. Through going beyond simple metrics of quantity, such as percentage green and blue cover, it is now possible to explore the extent to which landscape quality helps to unpick the mixed evidence presented in the literature on the benefits of urban nature to human well-being. Here we present a landscape approach, employing remote sensing, GIS and data reduction techniques to map urban green infrastructure elements in a large U.K. city region. Comparison with existing urban datasets demonstrates considerable improvement in terms of coverage and thematic detail. The characterisation of landscapes, using census tracts as spatial units, and subsequent exploration of associations with social–ecological attributes highlights the further detail that can be uncovered by the approach. For example, eight urban landscape types identified for the case study city exhibited associations with distinct socioeconomic conditions accountable not only to quantities but also qualities of green and blue space. The identification of individual landscape features through simultaneous measures of land use and land cover demonstrated unique and significant associations between the former and indicators of human health and ecological condition. The approach may therefore provide a promising basis for developing further insight into processes and characteristics that affect human health and well-being in urban areas, both in the United Kingdom and beyond.
Strategy for Modular Tagged High-Throughput Amplicon Sequencing (2011)

The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.
Sonic hedgehog expression correlates with fundic gland differentiation in the adult gastrointestinal tract (2002)

We found a strong correlation between Shh expression and fundic gland differentiation. Our current study therefore provides evidence that in addition to its role in gastric epithelial development, Shh plays a unique role in gastric epithelial differentiation in adults.
Molecular basis of AMP deaminase deficiency in skeletal muscle. (1992)

AMP deaminase (AMPD; EC 3.5.4.6) is encoded by a multigene family in mammals. The AMPD1 gene is expressed at high levels in skeletal muscle, where this enzyme is thought to play an important role in energy metabolism. Deficiency of AMPD activity in skeletal muscle is associated with symptoms of a metabolic myopathy. Eleven unrelated individuals with AMPD deficiency were studied, and each was shown to be homozygous for a mutant allele characterized by a C----T transition at nucleotide 34 (codon 12 in exon 2) and at nucleotide 143 (codon 48 in exon 3). The C----T transition at codon 12 results in a nonsense mutation predicting a severely truncated AMPD peptide. Consistent with this prediction, no immunoreactive AMPD1 peptide is detectable in skeletal muscle of these patients. This mutant allele is found in 12% of Caucasians and 19% of African-Americans, whereas none of the 106 Japanese subjects surveyed has this mutant allele. We conclude from these studies that this mutant allele is present at a sufficiently high frequency to account for the 2% reported incidence of AMPD deficiency in muscle biopsies. The restricted distribution and high frequency of this doubly mutated allele suggest it arose in a remote ancestor of individuals of Western European descent.
Growth-regulated synthesis and secretion of biologically active nerve growth factor by human keratinocytes. (1991)

Nerve growth factor (NGF) transcripts were identified in normal human keratinocytes in primary and secondary culture. The expression of the NGF mRNA was strongly down-regulated by corticosteroids and was maximal when keratinocytes were in the exponential phase of growth. Immunofluorescence studies on growing keratinocytes colonies and on elutriated keratinocytes obtained from growing colonies and mature stratified epithelium showed specific staining of the Golgi apparatus only in basal keratinocytes in the exponential phase of growth. The keratinocyte-derived NGF was secreted in a biologically active form as assessed by neurite induction in sensory neurons obtained from chick embryo dorsal root ganglia. Based on these data we suggest that the basal keratinocyte is the cell synthesizing and secreting NGF in the human adult epidermis. The paracrine secretion of NGF by keratinocytes might have a major role in regulating innervation, lymphocyte function, and melanocyte growth and differentiation in epidermal morphogenesis as well as during wound healing.
Fc receptor-mediated antiviral antibodies (2009)

Fc-FcgammaR interactions play a critical role in the biological function of antibody and are likely to be instrumental in preventing or modulating lentiviral infection. Exploiting antibody responses that depend on Fc-FcgammaR interactions may help widen the breadth and increase the potency of vaccine-induced antibody. Although the importance of generating optimal Fab-antigen interactions cannot be overestimated, improving Fc-FcgammaR interactions through adjuvants or other strategies provides another option for improving HIV vaccines and immunotherapies.
Human Papilloma Virus (HPV)‐E6/E7 and Epidermal Growth Factor Receptor (EGF‐R) Protein Levels in Cervical Cancer and Cervical Intraepithelial Neoplasia (CIN) (2001)

We have established a highly specific and sensitive semi-quantitative immunofluorescent antibody assay for measuring levels of HPV-E6/E7 proteins and EGF-R in archival cervical biopsies. Our data suggest an association between HPV-E6/E7 and EGF-R.
Human life engineering in daily life (2010)

Ergonomics (or Human Factors) are applied to make human life convenience and comfort according to precise observation and understanding of human characteristics and functions. In these fields of studies, basic and key technologies have been human centered design towards the improvement of quality of daily life. Living in our home, working in the office and moving in society are all in our daily affairs and life itself. In these circumstances, mobile support for human is one of the great issues in human centered design. To make better designs for driver support, first of all, a precise measurement of behaviors and understanding of the phenomena are also necessary to apply and fit with personal behaviors. In this paper the recent technical trends of driving behavioral analysis are reviewed for driving support systems and, above all, the examples of advanced behavioral analysis are shown for predicting the application for future active safety as well as suggesting the research topics for future development of driving support.
Selective removal of alpha heavy-chain glycosylation sites causes immunoglobulin A degradation and reduced secretion. (1988)

The importance of carbohydrate in the secretion of immunoglobulin A (IgA) has previously been suggested by results of studies with tunicamycin, which prevents N-linked glycosylation of all cell glycoproteins. To directly evaluate the role of individual oligosaccharides in the secretion of IgA, we have used site-directed mutagenesis to selectively eliminate the two N-linked attachment sites reported to be glycosylated in alpha heavy chains. Transfected wild-type and mutant alpha genes were expressed in kappa light-chain-producing MPC-11 variant myeloma cells, and secretion kinetics of the IgAs were compared. Removal of either or both glycosylation sites led to intracellular alpha heavy-chain degradation and a 90 to 95% inhibition of IgA secretion. These results reveal that both N-linked oligosaccharides of the alpha heavy chain are essential for intracellular stability and normal secretion of IgA. This suggests that the key function of carbohydrate here is to maintain proper conformation of the glycoprotein. We also found that when expressed in the MPC-11 variant cells, alpha heavy chains were glycosylated at a third, normally unused site.
An Effective Method of On-Resin Disulfide Bond Formation in Peptides (2005)

ADVERTISEMENT RETURN TO ISSUEPREVReportNEXTAn Effective Method of On-Resin Disulfide Bond Formation in PeptidesAmit K. Galande, Ralph Weissleder, and Ching-Hsuan TungView Author Information Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129 Cite this: J. Comb. Chem. 2005, 7, 2, 174–177Publication Date (Web):February 19, 2005Publication History Received12 October 2004Published online19 February 2005Published inissue 1 March 2005https://pubs.acs.org/doi/10.1021/cc049839rhttps://doi.org/10.1021/cc049839rbrief-reportACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views3940Altmetric-Citations40LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cyclization,Disulfides,Organic polymers,Peptides and proteins,Thiols Get e-Alerts
Effect of inflatable plastic splints on blood flow. (1966)

Inflatable plstic splints have been in use for some time in the United States for the emergency splinting of fracures;t
The Future of Genetic Studies of Complex Human Diseases (1996)

The identification of the genetic basis of complex human diseases such as schizophrenia and diabetes has proven difficult. In their Perspective, Risch and Merikangas propose that we can best accomplish this goal by combining the power of the human genome project with association studies, a method for determining the basis of a genetic disease.
Collagen fibril assembly during postnatal development and dysfunctional regulation in the lumican‐deficient murine cornea (2006)

The transparent cornea is the outer barrier of the eye and is its major refractive surface. Development of a functional cornea requires a postnatal maturation phase involving development, growth and organization of the stromal extracellular matrix. Lumican, a leucine-rich proteoglycan, is implicated in regulating assembly of collagen fibrils and the highly organized extracellular matrix essential for corneal transparency. We investigated the regulatory role(s) of lumican in fibril assembly during postnatal corneal development using wild type (Lum+/+) and lumican-null (Lum-/-) mice. In Lum+/+ mice, a regular architecture of small-diameter fibrils is achieved in the anterior stroma by postnatal day 10 (P10), while the posterior stroma takes longer to reach this developmental maturity. Thus, the anterior and the posterior stroma follow distinct developmental timelines and may be under different regulatory mechanisms. In Lum-/- mice, it is the posterior stroma where abnormal lateral associations of fibrils and thicker fibrils with irregular contours are evident as early as P10. In contrast, the anterior stroma is minimally perturbed by the absence of lumican. In Lum+/+ mice, lumican is expressed throughout the developing stroma at P10, with strong expression limited to the posterior stroma in the adult. Therefore, the posterior stroma, which is most vulnerable to lumican-deficiency, demonstrates an early developmental defect in fibril structure and architecture in the Lum-/- mouse. These defects underlie the reported increased light scattering and opacity detectable in the adult. Our findings emphasize the early regulation of collagen structure by lumican during postnatal development of the cornea.
The association of sex hormone levels with poor mobility, low muscle strength and incidence of falls among older men and women (2005)

It can be concluded that low levels of sex hormones were associated with impaired mobility and low muscle strength in men, but not in women. Levels of sex hormones were not associated with the incidence of falls neither in men, nor in women.
科学，即生活——第28届全国青少年科技创新大赛侧记 (2013)

小编从小就是一个理科“废柴”，所以对于数学、物理和化学成绩优异的同学，那是相当地羡慕，对于文科理科都好到离谱的学霸，更是只有仰望的份儿。那些单调的数字、复杂的公式、危险的实验，还有云山雾罩的定理，他们到底是怎么样钻进去的呢？因为这个疑问，所以对沉迷科学研究的同学，总是有着一种“脑回路离普通生活有距离”的印象。
Crystal structures of a rat anti-CD52 (CAMPATH-1) therapeutic antibody fab fragment and its humanized counterpart (1998)

The CAMPATH-1 family of antibodies are able systematically to lyse human lymphocytes with human complement by targeting the small cell-surface glycoprotein CD52, commonly called the CAMPATH-1 antigen. These antibodies have been used clinically for several years, providing therapy for patients with a variety of immunologically mediated diseases. We report here the first X-ray crystallographic analyses of a Fab fragment from a rat antibody, the original therapeutic monoclonal CAMPATH-1G and its humanized counterpart CAMPATH-1H, into which the six complementarity-determining regions of the rat antibody have been introduced. These structures have been refined at 2.6 A and 3.25 A resolution, respectively. The VL domains of adjacent molecules of CAMPATH-1H form a symmetric dimer within the crystals with an inter-molecular extended beta-sheet as seen in light chain dimers of the kappa class. Crystals of CAMPATH-1G have translational pseudo-symmetry. Within the antibody-combining sites, which are dominated by the protrusion of LysH52b and LysH53 from hypervariable loop H2, the charge distribution and overall integrity are highly conserved, but large changes in the position of loop H1 are observed and an altered conformation of loop H2. The major determinants of this are framework residues H71 and H24, whose identity differs in these two antibodies. These structures provide a detailed structural insight into the transplantation of an intact antibody-combining site between a rodent and a human framework, and provide an increased understanding of the specificity and antigen affinity of this pair of CAMPATH-1 antibodies for CD52. This study forms the structural basis for future modification and design of more effective antibodies to this important antigen.
A robust low complexity voice activity detection algorithm for speech communication systems (2002)

We present a robust voice activity detection algorithm which enables further bit rate reduction for practical speech communication systems. The voice activity decision is based on a set of energy and spectral measures. The local frame measures are compared to a long term estimation of the noise. The voice activity decision is obtained using a pattern recognition approach with additional decision smoothing. This algorithm was adopted as part of Annex B for the ITU Recommendation G.729.
Sir2p suppresses recombination of replication forks stalled at the replication fork barrier of ribosomal DNA in Saccharomyces cerevisiae (2003)

In the ribosomal DNA (rDNA) of Saccharomyces cerevisiae replication forks progressing against transcription stall at a polar replication fork barrier (RFB) located close to and downstream of the 35S transcription unit. Forks blocked at this barrier are potentially recombinogenic. Plasmids bearing the RFB sequence in its active orientation integrated into the chromosomal rDNA in sir2 mutant cells but not in wild-type cells, indicating that the histone deacetylase silencing protein Sir2 (Sir2p), which also modulates the aging process in yeast, suppresses the recombination competence of forks blocked at the rDNA RFB. Orientation of the RFB sequence in its inactive course or its abolition by FOB1 deletion avoided plasmid integration in sir2 mutant cells, indicating that stalling of the forks in the plasmid context was required for recombination to take place. Altogether these results strongly suggest that one of the functions of Sir2p is to modulate access of the recombination machinery to the forks stalled at the rDNA RFB.
A compact, high-speed, and low-cost hyperspectral imager (2012)

Although the potential of hyperspectral imaging has been demonstrated for several applications, using laboratory setups in research environments, its adoption by industry has so far been limited due to the lack of high speed, low cost and compact hyperspectral cameras. To bridge the gap between research and industry, we present a novel hyperspectral sensor that integrates a wedge filter on top of a standard CMOS sensor. To enable the low-cost processing of a microscopic wedge filter, we have introduced a design that is able to compensate for process variability. The result is a compact and fast hyperspectral camera made with low-cost CMOS process technology. The current prototype camera acquires 100 spectral bands over a spectral range from 560 nm to 1000 nm, with a spectral resolution better than 10 nm and a spatial resolution of 2048 pixels per line. The speed is 180 frames per second at illumination levels as typically used in machine vision. The prototype is a hyperspectral line scanner that acquires 16 lines per spectral band in parallel on a 4 MPixel sensor. The theoretic line rate for this implementation is thus 2880 lines per second.
Colesevelam hydrochloride: a specifically engineered bile acid sequestrant (2008)

AbstractThere is a well established association between serum cholesterol and coronary heart disease and this relationship has been found to be related to concentration and is continuous over the entire distribution of cholesterol levels. Recent studies have shown that intensive lipid lowering is beneficial and more stringent guidelines are advocated to improve the clinical outcome. Although statins are the first-line agents for the treatment of hypercholesterolemia, often it is evident that combination therapy is required to achieve the desired reduction in LDL-cholesterol (LDL-C). Niacin and bile acid sequestrants were among the first lipid-lowering drugs developed to lower LDL-C and have been established to be effective in both monotherapy and combination therapy. However, tolerability and compliance issues have limited the use of the traditional bile acid sequestrants. Colesevelam hydrochloride is the newest bile acid sequestrant and shows reduced adverse effects, improved tolerance and better compliance.Keywords: bile acid sequestrantcardiovascularcholesterolcolesevelam HCldiabetes
NF-κB inducing kinase is a therapeutic target for systemic lupus erythematosus (2018)

NF-κB-inducing kinase (NIK) mediates non-canonical NF-κB signaling downstream of multiple TNF family members, including BAFF, TWEAK, CD40, and OX40, which are implicated in the pathogenesis of systemic lupus erythematosus (SLE). Here, we show that experimental lupus in NZB/W F1 mice can be treated with a highly selective and potent NIK small molecule inhibitor. Both in vitro as well as in vivo, NIK inhibition recapitulates the pharmacological effects of BAFF blockade, which is clinically efficacious in SLE. Furthermore, NIK inhibition also affects T cell parameters in the spleen and proinflammatory gene expression in the kidney, which may be attributable to inhibition of OX40 and TWEAK signaling, respectively. As a consequence, NIK inhibition results in improved survival, reduced renal pathology, and lower proteinuria scores. Collectively, our data suggest that NIK inhibition is a potential therapeutic approach for SLE.
Dendritic Chelating Agents. 1. Cu(II) Binding to Ethylene Diamine Core Poly(amidoamine) Dendrimers in Aqueous Solutions (2004)

This paper describes an investigation of the uptake of Cu(II) by poly(amidoamine) (PAMAM) dendrimers with an ethylenediamine (EDA) core in aqueous solutions. We use bench scale measurements of proton and metal ion binding to assess the effects of (i) metal ion-dendrimer loading, (ii) dendrimer generation/terminal group chemistry, and (iii) solution pH on the extent of binding of Cu(II) in aqueous solutions of EDA core PAMAM dendrimers with primary amine, succinamic acid, glycidol, and acetamide terminal groups. We employ extended X-ray absorption fine structure (EXAFS) spectroscopy to probe the structures of Cu(II) complexes with Gx-NH2 EDA core PAMAM dendrimers in aqueous solutions at pH 7.0. The overall results of the proton and metal ion binding measurements suggest that the uptake of Cu(II) by EDA core PAMAM dendrimers involves both the dendrimer tertiary amine and terminal groups. However, the extents of protonation of these groups control the ability of the dendrimers to bind Cu(II). Analysis of the EXAFS spectra suggests that Cu(II) forms octahedral complexes involving the tertiary amine groups of Gx-NH2 EDA core PAMAM dendrimers at pH 7.0. The central Cu(II) metal ion of each of these complexes appears to be coordinated to 2-4 dendrimer tertiary amine groups located in the equatorial plane and 2 axial water molecules. Finally, we combine the results of our experiments with literature data to formulate and evaluate a phenomenological model of Cu(II) uptake by Gx-NH2 PAMAM dendrimers in aqueous solutions. At low metal ion-dendrimer loadings, the model provides a good fit of the measured extent of binding of Cu(II) in aqueous solutions of G4-NH2 and G5-NH2 PAMAM dendrimers at pH 7.0.
[Disturbances in the development of various hippocampal fields in rats as a long-term effect of acute perinatal hypoxia]. (2010)

The aim of this study was to determine the long-term effects of acute normobaric hypoxia in the perinatal period on the development of a hippocampal formation in rats. The experiments performed have shown that the exposure to hypoxia on postnatal day 2 resulted in a significant damage to the hippocampal field structure. Differential field sensitivity to the damaging factor was also detected. At postnatal day 20, the cell death was found in all the fields and the thinning of pyramidal neuron layers was observed. The neuronal death was mostly expressed in CA4 and CA3 fields. At postnatal day 30, the neuronal death remained significant in CA4 field, while it was reduced in CA3 field and was not found in CA1 field, however, in fascia dentata the death of neurons of the granular layers was increased. Along with it, the dimensions of the pyramidal neuron cell bodies were reduced in all the hippocampal fields. Also, in all the hippocampal fields, the activation of astrocytic reaction was found, which was more expressed in CA4 field, where gliosis was found to remain until prepuberal age (day 30).