gebhart/patrepeval huggingface dataset:
Identification of Six Novel Autophosphorylation Sites on Fibroblast Growth Factor Receptor 1 and Elucidation of Their Importance in Receptor Activation and Signal Transduction (1996)

Fibroblast growth factor receptor (FGFR) activation leads to receptor autophosphorylation and increased tyrosine phosphorylation of several intra cellular proteins. We have previously shown that autophosphorylated tyrosine 766 in FGFR1 serves as a binding site for one of the SH2 domains of phospholipase Cy and couples FGFR1 to phosphatidylinositol hydrolysis in several cell types. In this report, we describe the identification of six additional autophosphorylation sites (Y-463, Y-583, Y-585, Y-653, Y-654 and Y-730) on FGFR1. We demonstrate that autophosphorylation on tyrosines 653 and 654 is important for activation of tyrosine kinase activity of FGFR1 and is therefore essential for FGFR1-mediated biological responses. In contrast, autophosphorylation of the remaining four tyrosines is dispensable for FGFR1-mediated mitogen-activated protein kinase activation and mitogenic signaling in L-6 cells as well as neuronal differentiation of PC12 cells. Interestingly, both the wild-type and a mutant FGFR1 (FGFR1-4F) are able to phosphorylate Shc and an unidentified Grb2-associated phosphoprotein of 90 kDa (pp90). Binding of the Grb2/Sos complex to phosphorylated Shc and pp90 may therefore be the key link between FGFR1 and the Ras signaling pathway, mito-genesis, and neuronal differentiation.
Monoclonal anti-tumor necrosis factor antibody renders non-obese diabetic mice hypersensitive to irradiation and enhances insulitis development (1992)

In attempt to evaluate biological roles of tumor necrosis factor (TNF), we studied the effects of anti-TNF mAb in non-obese diabetic (NOD) mice. Anti-murine TNF mAb rendered NOD mice hypersensitive to the lethal effects of radiation and prevented the reconstitution of lethally irradiated mice with adoptively transferred lymphocytes. While TNF-alpha reduced the incidence of diabetes development in the adoptive transfer system even when given 6 days post-transfer, mAb to TNF could not reduce or increase the incidence of diabetes compared to control mice. Administration of TNF-alpha for 4 or 8 weeks significantly reduced the incidence of spontaneous insulitis in NOD mice, while anti-TNF mAb given for 8 weeks increased the incidence of insulitis significantly.
Ex vivo characterization and isolation of rare memory B cells with antigen tetramers (2011)

Studying human antigen-specific memory B cells has been challenging because of low frequencies in peripheral blood, slow proliferation, and lack of antibody secretion. Therefore, most studies have relied on conversion of memory B cells into antibody-secreting cells by in vitro culture. To facilitate direct ex vivo isolation, we generated fluorescent antigen tetramers for characterization of memory B cells by using tetanus toxoid as a model antigen. Brightly labeled memory B cells were identified even 4 years after last immunization, despite low frequencies ranging from 0.01% to 0.11% of class-switched memory B cells. A direct comparison of monomeric to tetrameric antigen labeling demonstrated that a substantial fraction of the B-cell repertoire can be missed when monomeric antigens are used. The specificity of the method was confirmed by antibody reconstruction from single-cell sorted tetramer(+) B cells with single-cell RT-PCR of the B-cell receptor. All antibodies bound to tetanus antigen with high affinity, ranging from 0.23 to 2.2 nM. Furthermore, sequence analysis identified related memory B cell and plasmablast clones isolated more than a year apart. Therefore, antigen tetramers enable specific and sensitive ex vivo characterization of rare memory B cells as well as the production of fully human antibodies.
Presence of a vertebrate fibrinogen-like sequence in an echinoderm. (1990)

Sequence comparisons of the three homologous polypeptide chains that compose vertebrate fibrinogens imply that the molecule evolved before the divergence of vertebrates and invertebrates, but, to our knowledge, no protein resembling vertebrate fibrinogen has even been reported from an invertebrate. We used primers based on sequences conserved between lamprey and human fibrinogens and applied the polymerase chain reaction (PCR) to cDNA preparations from various invertebrates. A fibrinogen-like sequence was identified in cDNA prepared from the soft tissues of a sea cucumber, parastichopus parvimensis. The PCR-prepared material was then used to clone two closely related mRNA sequences from a sea cucumber soft tissue cDNA library. The putative fibrinogen-related proteins, FReP-A and FReP-B, correspond to the carboxyl-terminal two-thirds of vertebrate fibrinogen beta and gamma chains. Computer comparisons of various fibrinogen-related sequences indicate that the sea cucumber proteins diverged before the beta-gamma gene duplication.
The Ligand-Binding Domains of the Thyroid Hormone/Retinoid Receptor Gene Subfamily Function in Vivo To Mediate Heterodimerization, Gene Silencing, and Transactivation (1995)

The ligand-binding domains (LBDs) of the thyroid/retinoid receptor gene subfamily contain a series of heptad motifs important for dimeric interactions. This subfamily includes thyroid hormone receptors (T3Rs), all-trans retinoic acid (RA) receptors (RARs), 9-cis RA receptors (RARs and retinoid X receptors [RXRs]), the 1,25-dihydroxyvitamin D3 receptor (VDR), and the receptors that modulate the peroxisomal beta-oxidation pathway (PPARs). These receptors bind to their DNA response elements in vitro as heterodimers with the RXRs. Unliganded receptors in vivo, in particular the T3Rs, can mediate gene silencing and ligand converts these receptors into a transcriptionally active form. The in vivo interactions of these receptors with RXR were studied by using a GAL4-RXR chimera containing the yeast GAL4 DNA-binding domain and the LBD of RXR beta. GAL4-RXR activates transcription from GAL4 response elements in the presence of 9-cis RA. Unliganded T3R, which does not bind or activate GAL4 elements, represses the activation of GAL4-RXR by 9-cis RA in HeLa cells. However, addition of T3 alone leads to transcriptional activation. These findings suggest that T3R can repress or activate transcription while tethered to the LBD of GAL4-RXR and that heterodimerization can occur in vivo without stabilization by hormone response elements. Similar ligand-dependent activation was observed in HeLa cells expressing RAR, VDR, or PPAR and in GH4C1 cells from endogenous receptors. Replacement of the last 17 amino acids of the LBD of RXRbeta with the 90-amino-acid transactivating domain of the herpes simplex virus VP16 protein leads to a GAL4 constitutive activator that is repressed by wild-type T3R but not by a ninth heptad mutant that does not form heterodimers. This finding suggests that the ninth heptad or T3R is important for gene silencing and that the LBD of RXR does not exhibit silencing activity. This conclusion was verified with GAL4-LBD chimeras and with wild-type receptors in assays using appropriate response elements. These studies indicate that the LBD has diverse functional roles in gene regulation.
Vacuum ultraviolet circular dichroism of protected homooligomers derived from L-leucine (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVacuum ultraviolet circular dichroism of protected homooligomers derived from L-leucineMargaret M. Kelly, E. S. Pysh, G. M. Bonora, and C. TonioloCite this: J. Am. Chem. Soc. 1977, 99, 10, 3264–3266Publication Date (Print):May 1, 1977Publication History Published online1 May 2002Published inissue 1 May 1977https://pubs.acs.org/doi/10.1021/ja00452a010https://doi.org/10.1021/ja00452a010research-articleACS PublicationsRequest reuse permissionsArticle Views78Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Expression of herpes simplex virus type 1 DNA polymerase in Saccharomyces cerevisiae and detection of virus-specific enzyme activity in cell-free lysates (1988)

The herpes simplex virus type 1 (HSV-1) (strain 17) DNA polymerase gene has been cloned into an Escherichia coli-yeast shuttle vector fused to the galactokinase gene (GAL-1) promoter. Genes controlled by the GAL-1 promoter are induced by galactose, uninduced by raffinose, and repressed by glucose. Cell extracts from a strain of Saccharomyces cerevisiae harboring this vector (Y-MH202, expresser cells) grown in the presence of galactose and assayed in high salt (100 mM ammonium sulfate) contained a novel DNA polymerase activity. No significant high-salt DNA polymerase activity was detected in extracts from expresser cells grown in the presence of raffinose or in extracts from control cells containing the E. coli-yeast shuttle vector without the HSV-1 DNA polymerase gene grown in the presence of raffinose of galactose. Immunoblot analysis of the cell extracts by using a polyclonal rabbit antiserum prepared against a highly purified HSV-1 DNA polymerase preparation revealed the specific induction of the HSV-1 approximately 140-kilodalton DNA polymerase polypeptide in expresser cells grown in galactose. Extracts from the same cells grown in raffinose or control cells grown in either raffinose or galactose did not contain this immunoreactive polypeptide. The high-salt DNA polymerase activity in the extracts from expresser cells grown in galactose was inhibited greater than 90% by either acyclovir triphosphate or aphidicolin, as expected for HSV-1 DNA polymerase. In addition, the high-salt polymerase enzyme activity could be depleted from extracts by immunoprecipitation by using purified immunoglobulin G from this same polyclonal rabbit antiserum. These results demonstrate the successful expression of functional HSV-1 DNA polymerase enzyme in S. cerevisiae.
Humoral immune response to the entire human immunodeficiency virus envelope glycoprotein made in insect cells. (1987)

The human immunodeficiency virus envelope gene was expressed in insect cells by using a Baculovirus expression vector. The protein has an apparent molecular mass of 160 kDa, appears on the surface of infected insect cells, and does not appear to be cleaved to glycoproteins gp120 and gp41. Goats immunized with the 160-kDa protein have high titers of antibody that neutralizes virus infection as measured by viral gene expression or cell cytolysis. In addition, immune sera can block fusion of human immunodeficiency virus-infected cells in culture. Both neutralization and fusion-blocking activities are bound to and eluted from immobilized gp120.
On-chip metamaterial transmission-line based on stacked split-ring resonator for millimeter-wave LSIs (2009)

This paper proposes a method to realize on-chip metamaterial transmission-line. Since the resonant frequency of split-ring resonator (SRR) depends on the size strongly, it is difficult to realize metamaterial transmission-line in integrated circuits. If we implement a conventional SRR to on-chip transmission-line, the resonant frequency is around 200 GHz. We propose a stacked SRR using on-chip multi-layer interconnect. The stacked SRR is evaluated by a field-solver and the experimental results verify that the stacked SRR can realize the resonant frequency below 60 GHz.
Near‐Infrared Light‐Mediated Nanoplatforms for Cancer Thermo‐Chemotherapy and Optical Imaging (2013)

While thermo-chemotherapy has proved to be effective in optimizing the efficacies of cancer treatments, traditional chemotherapy is subject to adverse side effects and heat delivery is often challenging in operation. Some photothermal inorganic nanoparticles responsive to near infrared light provide new opportunities for simultaneous and targeted delivery of heat and chemotherapeutics to the tumor sites in pursuit of synergistic effects for efficacy enhancement. The state of the art of nanoparticle-induced thermo-chemotherapy is summarized and the advantages and challenges of the major nanoplatforms based on gold nanoparticles, carbon nanomaterials, palladium nanosheets, and copper-based nanocrystals are highlighted. In addition, the optical-imaging potentials of the nanoplatforms that may endow them with imaging-guided therapy and therapeutic-result-monitoring capabilities are also briefly discussed.
Single-shot measurement of the intensity and phase of a femtosecond UV laser pulse with frequency-resolved optical gating (1994)

We extend to the ultraviolet frequency-resolved optical gating a new method for measuring the intensity and phase evolution of an individual ultrashort pulse without assumption. Using frequency-resolved optical gating, we measure a 310-fs, 308-nm pulse, whose phase is approximately cubic in time. We show that this phase distortion probably results from self-phase modulation and amplifier detuning.
Determination of Multiresidue Pesticides in Green Tea by Using a Modified QuEChERS Extraction and Ion-Trap Gas Chromatography/Mass Spectrometry (2010)

The pesticide residues in exported and imported tea products must not exceed the maximum residue limits (MRLs) regulated by the import countries. Tea is a complex matrix that obfuscates the determination of pesticide residues. Many available methods for multiresidue pesticide analysis of tea are time-consuming and require many cleanup steps. The objective of this study was to develop a simple multiresidue method by using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and ion-trap GC/MS/MS, which can identify, confirm, and quantify pesticides in complex matrixes. A tea product was homogenized with water, and the pesticides were extracted with acetonitrile containing 1% acetic acid. The extract was subjected to centrifugation, initial cleanup with dispersive SPE (dSPE), solvent exchange, and final cleanup with dSPE. Diethyl-d10-parathion and triphenyl phosphate were used as the internal standard and surrogate, respectively. The final extract was injected into an ITQ 700 gas chromatograph/mass spectrometer. Quantitation of individual pesticides was based on matrix-matched calibration curves with a correlation coefficient of > 0.9930 for the 22 pesticides selected for the study. The recoveries of the 22 pesticides ranged from 78 to 115%, except those for diazinon (130%) and malathion (122%), with an average RSD of 8.7%. The LOD values of all of the pesticides, except for terbufos, were below the MRLs set by the European Union and Japan.
A Non-Cannabinoid Immunogen Used to Elicit Antibodies with Broad Cross-Reactivity to Cannabinoid Metabolites (1998)

A benzpyran derivative was linked to the lysines of bovine thyroglobulin (BTG) where 69% of the available lysines were modified. This derivative was designed to elicit antibodies that were directed towards the conserved epitopes of cannabinoid metabolites that appear in urine. Polyclonal antibodies from sheep and goats and murine monoclonal antibodies were generated using this immunogen. The cross-reactivity of the antibodies was compared with antibodies generated from the more traditional phenolic-linked or 9-linked immunogens. An ELISA assay was developed using delta 9-11-nor-9-carboxy-tetrahydrocannabinol (COOH-THC) to obtain a standard curve. The antibodies generated using the benzpyran immunogen showed an average of two to three times higher cross-reactivity towards 11-OH-delta 9-THC, 8 beta-OH-delta 9-THC, 8 alpha-OH-delta 9-THC, 11-OH-delta 8-THC, and 8 beta,11-di-OH-delta 9-THC than antibodies that were generated by traditional cannabinoid immunogens. The selectivity of the benzpyran-elicited antibodies was also compared with antibodies derived from traditional immunogens using clinical urine samples that were confirmed for cannabinoids by GC/MS. The total cross-reactive cannabinoid values obtained with the benzpyran-elicited antibodies were 49% higher than the values obtained using the traditional immunogen structures. The benzpyran immunogen-induced antibodies exhibited the same low cross-reactivity for non-structurally related compounds as antibodies derived from traditional immunogens. The novel benzpyran immunogen used in this study is the first non-cannabinoid immunogen used to generate cannabinoid-selective antibodies and demonstrates the usefulness of such a structure in developing broadly cross-reactive cannabinoid antibodies.
High-Spin Molecules: Iron(III) Incorporation into [Mn12O12(O2CMe)16(H2O)4] To Yield [Mn8Fe4O12(O2CMe)16(H2O)4] and Its Influence on the S = 10 Ground State of the Former (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh-Spin Molecules: Iron(III) Incorporation into [Mn12O12(O2CMe)16(H2O)4] To Yield [Mn8Fe4O12(O2CMe)16(H2O)4] and Its Influence on the S = 10 Ground State of the FormerAnn R. Schake, Hui-Lien Tsai, Robert J. Webb, Kirsten Folting, George Christou, and David N. HendricksonCite this: Inorg. Chem. 1994, 33, 26, 6020–6028Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://doi.org/10.1021/ic00104a009RIGHTS & PERMISSIONSArticle Views299Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
High power (2.1 W) 10-stripe AlGaAs laser arrays with Si disordered facet windows (1986)

Silicon impurity induced disordering has been used to fabricate lasers with reduced facet absorption (facet windows) exhibiting enhanced catastrophic facet damage levels over comparable nonwindow devices. Power levels of 1.2 W cw were obtained from uncoated output facet devices and 2.1 W cw were obtained for a device with a coated output facet. Evidence is presented that the window region formed by silicon diffusion is a low-loss waveguide which confines the propagating wave, increasing the efficiency of the device.
Image Sequence Compression Using a Pel-Recursive Motion-Compensated Technique (1987)

This paper presents and analyzes a pel-recursive, motioncompensated, image sequence compression algorithm [1]. The analysis retains all the terms of the Taylor's series expansion and yields a set of equations for which the convergence criteria and the convergence rate of the motion estimate are more easily seen. The existing motion prediction schemes are also reviewed and a new motion prediction scheme is presented which is shown to be superior to the existing schemes. Simulations run on actual image sequences to verify the analytical results indicate that implementing the analytical model as opposed to the generally used heuristic technique does yield a decrease in the information rate and the computational requirements. Simulation results also are included which use the "projection-along-the-motiontrajectory" or PAMT prediction scheme. Third, zeroth-order entropy encoding is shown to reduce the bit rate on the order of 12 percent, and to reduce the mean square error in the reconstructed images on the order of 60 percent when compared to first-order entropy encoding. Fourth, field-to-field motion prediction is compared to frame-to-frame motion prediction.
Cumulative rate of relapse of lupus nephritis after successful treatment with cyclophosphamide (1996)

A selected population of SLE patients may have long-term remission of renal disease following successful CYC therapy. Patients in whom CYC treatment is delayed or who are young at the time of nephritis onset are at increased risk of relapse.
Theory of communication. Part 3: Frequency compression and expansion (1946)

It is suggested that it may be possible to transmit speech and music in much narrower wavebands than was hitherto thought necessary, not by clipping the ends of the waveband, but by condensing the information. Two possibilities of more economical transmission are discussed. Both have in common that the original waveband is compressed in transmission and re-expanded to the original width in reception. In the first or “kinematical” method a temporary or permanent record is scanned by moving slits or their equivalents, which replace one another in continuous succession before a “window.” Mathematical analysis is simplest if the transmission of the window is graded according to a probability function. A simple harmonic oscillation is reproduced as a group of spectral lines with frequencies which have an approximately constant ratio to the original frequency. The average departure from the law of proportional conversion is in inverse ratio to the time interval in which the record passes before the window. Experiments carried out with simple apparatus indicate that speech can be compressed into a frequency band of 800 or even 500 c/s without losing much of its intelligibility. There are various possibilities for utilizing frequency compression in telephony by means of the “kinematical” method.In a second method the compression and expansion are carried out electrically, without mechanical motion. This method consists essentially in using non-sinusoidal carriers, such as repeated probability pulses, and local oscillators producing waves of the same type. It is shown that one variety of the electrical method is mathematically equivalent to the kinematical method of frequency conversion.
Wave manipulation with designer dielectric metasurfaces (2014)

The concept of an ultra-thin metasurface made of single layer of only-dielectric disks for successful phase control over a full range is demonstrated. Conduction loss is avoided compared to its plasmonic counterpart. The interaction of the Mie resonances of the first two modes of the dielectric particles, magnetic and electric dipoles, is tailored by the dimensions of the disks, providing required phase shift for the transmitted beam from 0° to 360°, together with high transmission efficiency. The successful performance of a beam-tilting array and a large-scale lens functioning at 195 THz demonstrates the ability of the dielectric metasurface that is thin and has also high efficiency of more than 80%. Such configurations can serve as outstanding alternatives for plasmonic metasurfaces especially that it can be a scalable design.