gebhart/patrepeval huggingface dataset:
CD70: A Potential Target in Breast Cancer? (2014)

CD70 is a co-stimulatory molecule involved in the immune response and also in cancer development and progression. Recent studies show that high CD70 expression in cancer cells may inhibit the anti-tumor response. Furthermore, CD70 expression has been reported as a predictive marker of resistance to chemotherapy in ovarian cancers. Some in vitro studies have shown that CD70 expression is epigenetically down-regulated through hypermethylation of its promoter during tumoral progression. This study evaluated the level of CD70 expression in surgical samples of breast invasive tumors and determined its correlation with CD70 promoter methylation. Twenty "luminal A" and 20 "basal-like" frozen samples from early breast tumors were retrospectively selected. CD70 expression was evaluated by quantitative real-time PCR. Total DNA was bisulfite-treated, and methylation levels of 5 consecutive CG sites present in the proximal region (-464, -421) of the promoter were assessed by pyrosequencing analysis. Statistical analyses were performed using the Mann-Whitney test. The median relative CD70 expression level was 0.37 and was significantly higher in the basal-like group (0.78 [0.24-31.7]) compared to the luminal A group (0.25 [0.03-1.83], p=0.0001). The median methylation level was 61%, with no significant difference between the basal-like (63%) and luminal A (58%) groups. No correlation was found between CD70 expression and CD70 methylation level. In this study, higher CD70 expression was observed in the basal-like group, but this expression was not related to promoter methylation. The higher expression in the poor-prognosis subgroup of patients makes CD70 a potential target for emerging anti-CD70 therapies.
Requirement for V α 14 NKT Cells in IL-12-Mediated Rejection of Tumors (1997)

A lymphocyte subpopulation, the Valpha14 natural killer T (NKT) cells, expresses both NK1.1 and a single invariant T cell receptor encoded by the Valpha14 and Jalpha281 gene segments. Mice with a deletion of the Jalpha281 gene segment were found to exclusively lack this subpopulation. The Valpha14 NKT cell-deficient mice could no longer mediate the interleukin-12 (IL-12)-induced rejection of tumors. Although the antitumor effect of IL-12 was thought to be mediated through natural killer cells and T cells, Valpha14 NKT cells were found to be an essential target of IL-12, and they mediated their cytotoxicity by an NK-like effector mechanism after activation with IL-12.
Immunocytochemical localization of vasoactive intestinal peptide and substance P in the colon from normal subjects and patients with inflammatory bowel disease. (1992)

Neuropeptides form a part of the brain-gut axis which may regulate gastrointestinal functions, including immune regulation. Various changes in the neuropeptides--most important, vasoactive intestinal peptide and substances P (VIP and SP)--have been described in inflammatory bowel disease. We employed a sensitive immunoperoxidase (avidin-biotin-peroxidase complex) technique, using anti-VIP and anti-SP antibodies to localize and compare the distribution of VIP and SP in the colon. Colon specimens from 19 normal subjects, eight patients with ulcerative colitis (UC), and eight with Crohn's disease (CD) were used. In the normal colon, VIP and SP immunoreactivity (IR) were localized in the muscularis mucosa, circular muscles, walls of blood vessels, nerve fibers, and some distinct cells, probably enterochromaffin cells. SP-IR was also present in the epithelial cells, mainly along the basolateral domain. VIP-IR was considerably diminished at all locations in patients with UC and CD. However, the SP-IR was increased in UC in the colonic epithelial cells along the basolateral areas. The SP-IR was intense in patients with CD, in the epithelium, the granulomas, cells lining the mucosal fissure, and in the muscle layers. In contrast to normals, SP-IR in patients with CD was observed both in the longitudinal and circular muscles. We conclude that VIP-IR and SP-IR are distributed widely in the mucosa, submucosa, and in the circular muscle in normal colon. VIP-IR is decreased in UC and CD, whereas SP-IR is increased in both, but more so in CD.
Progressive versus Catastrophic Disability: A Longitudinal View of the Disablement Process (1996)

Tracking the development of disability provides new and important insights into the disability experience in older men and women that are potentially relevant in planning preventive, intervention, and long-term care strategies.
Fibrous Hybrid of Graphene and Sulfur Nanocrystals for High-Performance Lithium–Sulfur Batteries (2013)

Graphene-sulfur (G-S) hybrid materials with sulfur nanocrystals anchored on interconnected fibrous graphene are obtained by a facile one-pot strategy using a sulfur/carbon disulfide/alcohol mixed solution. The reduction of graphene oxide and the formation/binding of sulfur nanocrystals were integrated. The G-S hybrids exhibit a highly porous network structure constructed by fibrous graphene, many electrically conducting pathways, and easily tunable sulfur content, which can be cut and pressed into pellets to be directly used as lithium-sulfur battery cathodes without using a metal current-collector, binder, and conductive additive. The porous network and sulfur nanocrystals enable rapid ion transport and short Li(+) diffusion distance, the interconnected fibrous graphene provides highly conductive electron transport pathways, and the oxygen-containing (mainly hydroxyl/epoxide) groups show strong binding with polysulfides, preventing their dissolution into the electrolyte based on first-principles calculations. As a result, the G-S hybrids show a high capacity, an excellent high-rate performance, and a long life over 100 cycles. These results demonstrate the great potential of this unique hybrid structure as cathodes for high-performance lithium-sulfur batteries.
An in vitro study of the effects of cell wall polysaccharides of potatoes on digestibilities of their starches. (1985)

1) 一般的に生食される山の芋 (Dioscorea Batatas), 消化がよいとされているじゃがいも (Solanum tubero-sum), 消化が悪いとされているさつまいも (IpomoeaBatatas) について, それぞれの細胞壁にとり囲まれた状態で加熱糊化されたデンプンの消化性を試験管内で比較した結果, いずれのいもの場合も細胞壁が存在しない陽合に比し, デンプンの消化率は低く, 消化抑制率は山の芋が最も低く, さつまいもが最も高い値を示した。2) 3種のいもの細胞壁構成多糖の構成比を検討した結果, じゃがいも, 山の芋では主としてペクチン, ヘミセルロースから成るのに対し, さつまいもでは, セルロースの含量が他のいもに比し, 顕著に高値を示した。また各種いもの細胞壁構成多糖の構成糖を比較すると, 発生学的に根に属するさつまいも, 山の芋ではグルコースの比率が高いのに対し, 茎に属するじゃがいもではグルコースのほか, ガラクトース, ラムノースの比率も高値を示した。一方, セルロースの比率が最も低い山の芋では細胞壁の構造のもろさが示された。3) それぞれのいもの細胞壁構成多糖による, α-アミラーゼの阻害性を検討した結果, いずれの場合も, 細胞壁構成多糖そのものによって, α-アミラーゼ作用が阻害を受ける事実は認められなかった。4) いもの種類によって, 細胞壁存在下のデンプンの消化性に差が認められたのは, 細胞壁構成多糖の量, 種類, 構造等の違いによって, 細胞内へのα-アミラーゼの透過性が影響を受けたためと考えられた。また, このことは生体内でのデンプンの消化性にも何らかの影響を与えるものと考えられた。
Histone Deacetylase-3 Modification of MicroRNA-31 Promotes Cell Proliferation and Aerobic Glycolysis in Breast Cancer and Is Predictive of Poor Prognosis (2018)

Our findings revealed that HDAC3 served as an oncogene that could promote cell proliferation and aerobic glycolysis and was predictive of a poor prognosis in breast cancer. HDAC3 participated in the cell proliferation of breast cancer, which may prove to be a pivotal epigenetic target against this devastating disease.
Bacterial biota in the human distal esophagus (2004)

The esophagus, like other luminal organs of the digestive system, provides a potential environment for bacterial colonization, but little is known about the presence of a bacterial biota or its nature. By using broad-range 16S rDNA PCR, biopsies were examined from the normal esophagus of four human adults. The 900 PCR products cloned represented 833 unique sequences belonging to 41 genera, or 95 species-level operational taxonomic units (SLOTU); 59 SLOTU were homologous with culture-defined bacterial species, 34 with 16S rDNA clones, and two were not homologous with any known bacterial 16S rDNA. Members of six phyla, Firmicutes, Bacteroides, Actinobacteria, Proteobacteria, Fusobacteria, and TM7, were represented. A large majority of clones belong to 13 of the 41 genera (783/900, 87%), or 14 SLOTU (574/900, 64%) that were shared by all four persons. Streptococcus (39%), Prevotella (17%), and Veilonella (14%) were most prevalent. The present study identified approximately 56-79% of SLOTU in this bacterial ecosystem. Most SLOTU of esophageal biota are similar or identical to residents of the upstream oral biota, but the major distinction is that a large majority (82%) of the esophageal bacteria are known and cultivable. These findings provide evidence for a complex but conserved bacterial population in the normal distal esophagus.
Effect of deposition conditions and composition on band offsets in atomic layer deposited HfxSi1−xOy on InGaZnO4 (2017)

HfO2 or HfxSi1−xO4 (consisting of HfO2/SiO2 multilayers) were deposited at 200 °C by atomic layer deposition (ALD) onto a-InGaZnO4 (IGZO) layers deposited by sputtering at room temperature and the band offsets in the HfxSi1−xOy/InGaZnO4 heterostructures determined by x-ray photoelectron spectroscopy. The values for HfxSi1−xOy/IGZO are different from those obtained when sputtering is used to deposit the dielectric, indicating that surface disordering or metal inclusions can play a role in the offsets. Similarly, the band offsets for SiO2 deposited on IGZO by ALD are larger than previous reports for the same system in which the SiO2 was deposited by plasma enhanced chemical vapor deposition. In that latter case, the effect of incorporated hydrogen in the SiO2 may play a role by reducing the bandgap. By contrast, there was no difference in the offsets in interfaces initiated with SiO2-first versus HfO2-first deposition and no effect of annealing the IGZO at the ALD-deposition temperature prior to dielectric deposition. The valence band offset for HfxSi1−xOy/IGZO was determined as a function of composition, with values ranging from 1.73 eV for pure SiO2 to 0.52 eV for pure HfO2. Across the entire composition range, the HfxSi1−xOy/InGaZnO4 system has a nested, or straddling, gap (type I) alignment.
Passive immunotherapy of tauopathy targeting pSer413‐tau: a pilot study in mice (2015)

These results indicate that pSer413 is a promising target in the treatment of tauopathy.
Efficacy and safety of incretin therapy in type 2 diabetes: systematic review and meta-analysis. (2007)

Incretin therapy offers an alternative option to currently available hypoglycemic agents for nonpregnant adults with type 2 diabetes, with modest efficacy and a favorable weight-change profile. Careful postmarketing surveillance for adverse effects, especially among the DPP4 inhibitors, and continued evaluation in longer-term studies and in clinical practice are required to determine the role of this new class among current pharmacotherapies for type 2 diabetes.
Synseeds: applications of synthetic seeds to crop improvement. (1993)

Part 1 Synthetic seed technology: desiccated synthetic seed, J. Janick, et al hydrated coatings for synthetic seeds, K. Redenbaugh, et al hydrophobic coatings for synthetic seeds, D. Friend immobilization of whole cells and somatic embryos - coating process and cell-matrix interaction, J.-N Barbotin, et al maturation of somatic embryos, C.H. Bornman molecular markers for plant embryos, C.H. Bornman molecular markers for plant embryos, P. Sterk and S.C. der Vries long-term preservation of somatic embryos, B. Florin, et al variability in somatic embryos, P.D.S. Caligari and S. Shohet bioreactor culture of somatic embryos for mass propagation of plants, S. Nishimura, et al field planting of synthetic seeds, J. Fujii, et al encapsulation machinery, R. Garrett synthetic seed patents, J.C. Weseman. Part 2 Case studies: synthetic seeds of alfalfa, B. McKersie and S. Bowley carrot somatic embryogenesis and its application to synthetic seeds, F. Molle, et al somatic embryogenesis and synthetic seed technology using carrot as a model system, X.-Q. Li, celery and lettuce, M. Sanada, et al the potential for synthetic soybean seed, J. Ranch liquid suspension culture production of orchard grass somatic embryos and their potential for the breeding of improved varieties, D.J. Gray, et al grape somatic embryo dormancy and quiescence - potential of dehydrated synthetic seeds for germplasm conservation, D.J. Gray and M. Compton studies on synthetic seeds of sandalwood and mulberry, V.A. Bapat somatic embryos of mango, R.E. Litz, et al somatic embryogenesis of spruce, D.R. Roberts, et al conclusions and future, Y. Le Deunff.
Growth and Survival of Heliothis virescens (Lepidoptera: Noctuidae) on Transgenic Cotton Containing a Truncated Form of the Delta Endotoxin Gene from Bacillus thuringiensis (1993)

Journal Article Growth and Survival of Heliothis virescens (Lepidoptera: Noctuidae) on Transgenic Cotton Containing a Truncated Form of the Delta Endotoxin Gene from Bacillus thuringiensis Get access Johnie N. Jenkins, Johnie N. Jenkins Crop Science Research Laboratory, USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762 Search for other works by this author on: Oxford Academic PubMed Google Scholar W. L. Parrott, W. L. Parrott Crop Science Research Laboratory, USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762 Search for other works by this author on: Oxford Academic PubMed Google Scholar J. C. McCarty, Jr., J. C. McCarty, Jr. Crop Science Research Laboratory, USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762 Search for other works by this author on: Oxford Academic PubMed Google Scholar F. E. Callahan, F. E. Callahan Crop Science Research Laboratory, USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762 Search for other works by this author on: Oxford Academic PubMed Google Scholar S. A. Berberich, S. A. Berberich Crop Science Research Laboratory, USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762 Search for other works by this author on: Oxford Academic PubMed Google Scholar Randy W. Deaton Randy W. Deaton Crop Science Research Laboratory, USDA-ARS, P.O. Box 5367, Mississippi State, MS 39762 Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 86, Issue 1, 1 February 1993, Pages 181–185, https://doi.org/10.1093/jee/86.1.181 Published: 01 February 1993 Article history Received: 13 November 1991 Accepted: 03 September 1992 Published: 01 February 1993
Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7 (2011)

To allow vaccination irrespective of HLA type, DNA vaccines encoding full-length antigens are required. However, here, we demonstrate that the immunogenicity of DNA vaccines encoding the full-length human papillomavirus (HPV) type 16 E7 and E6 proteins is highly reduced compared to vaccines encoding only the immunodominant epitope. Furthermore, the low remaining immunogenicity is essentially lost for both E7 and E6 when a nononcogenic "gene-shuffled" variant is utilized. To address these issues, we tested whether alterations in transgene design can restore the immunogenicity of full-length and gene-shuffled DNA vaccines. Remarkably, genetic fusion of E7 with tetanus toxin fragment C (TTFC) resulted in a dramatic increase in immunogenicity both for the full-length and the gene-shuffled version of E7. Moreover, the TTFC fusion vaccines were more immunogenic than a vaccine encoding a fusion of E7 and mycobacterial heat shock protein-70, which has recently been tested in a clinical trial. Interestingly, vaccination with these TTFC fusion vaccines also resulted in extremely persistent T-cell responses. The E7-specific CD8(+) T cells induced by TTFC fusion vaccines were functional in terms of IFN-γ production, formation of immunological memory, in vivo cytolytic activity and tumor eradication. Finally, we show that genetic fusion with TTFC also improves the immunogenicity of a gene-shuffled E6 DNA vaccine. These data demonstrate that genetic fusion with tetanus toxin fragment C can dramatically improve the immunogenicity of full-length and gene-shuffled DNA vaccines. The DNA fusion vaccines developed here will be evaluated for the treatment of HPV-positive carcinomas in future studies.
Enhanced production of arginine and urea by genetically engineered Escherichia coli K-12 strains (1997)

Escherichia coli strains capable of enhanced synthesis of arginine and urea were produced by derepression of the arginine regulon and simultaneous overexpression of the E. coli carAB and argI genes and the Bacillus subtilis rocF gene. Plasmids expressing carAB driven by their natural promoters were unstable. Therefore, E. coli carAB and argI genes with and without the B. subtilis rocF gene were constructed as a single operon under the regulation of the inducible promoter ptrc. Arginine operator sequences (Arg boxes) from argI were also cloned into the same plasmids for titration of the arginine repressor. Upon overexpression of these genes in E. coli strains, very high carbamyl phosphate synthetase, ornithine transcarbamylase, and arginase catalytic activities were achieved. The biosynthetic capacity of these engineered bacteria when overexpressing the arginine biosynthetic enzymes was 6- to 16-fold higher than that of controls but only if exogenous ornithine was present (ornithine was rate limiting). Overexpression of arginase in bacteria with a derepressed arginine biosynthetic pathway resulted in a 13- to 20-fold increase in urea production over that of controls with the parent vector alone; in this situation, the availability of carbamyl phosphate was rate limiting.
[Non-invasive instrumental method in uncontrollable post-partum hemorrhage (an alternative to hemostatic hysterectomy)]. (1998)

A new non-invasive instrumental technique which is useful to stop the most severe post-partum haemorrhages is described. This technique is proposed as an alternative to more invasive methods such as the binding of uterine or hypogastric arteries, which is itself a trauma, not always effective and could promote the obstetric shock. The method proposed by the authors is not invasive, can be performed in a few minutes and performed without the help of anaesthesia. Very encouraging results were achieved and have allowed to avoid the use of the so-called "haemostatic" hysterectomy.
<title>A Four Channel 10-Nanometer Spacing Wavelength Demultiplexer</title> (1983)

Results on the development of an experimental four channel fiber optics Wavelength Division Multiplexing (WDM) system with 10 nanometer channel spacing will be presented. Data on WDM demultiplexers utilizing narrow band interference filters will be reviewed. Results on studies of wavelength stability of semiconductor lasers for WDM will also be presented.
Design and Synthesis of Modified Quinolones as Antitumoral Acridones (1999)

The bacterial topoisomerase II (DNA gyrase) and the mammalian topoisomerase II represent the cellular targets for quinolone antibacterials and a wide variety of anticancer drugs, respectively. In view of the mechanistic similarities and sequence homologies exhibited by the two enzymes, tentative efforts to selectively shift from an antibacterial to an antitumoral activity was made by synthesizing a series of modified tricyclic quinolones, in which the essential 3-carboxylic function is surrogated by phenolic OH and the classic C-6 fluorine atom is replaced by a NH2 group. The resulting 7-amino-9-acridone derivatives were assayed for their antibacterial as well as cytotoxic activities. No antibacterial activity was found. On the other hand, many derivatives showed significant cytotoxic activity against both HL-60 and P388 leukemias and a wide panel of human and rodent solid tumor cells, derivatives 25 and 26 displaying the best overall antiproliferative activity. Against the LoVo cell line, derivative 25 exhibited higher cytotoxic effects than etoposide.
Structure and expression of the excision repair geneERCC6, involved in the human disorder Cockayne's syndrome group B (1993)

The human repair gene ERCC6--a presumed DNA (or RNA) helicase--has recently been found to function specifically in preferential nucleotide excision repair (NER). This NER subpathway is primarily directed towards repair of (the transcribed strand of) active genes. Mutations in the ERCC6 gene are responsible for the human hereditary repair disorder Cockayne's syndrome complementation group B, the most common form of the disease. In this report, the genomic organization and expression of this gene are described. It consists of at least 21 exons, together with the promoter covering a region of 82-90 kb on the genome. Postulated functional domains deduced from the predicted amino acid sequence, including 7 distinct helicase signatures, are--with one exception--encoded on separate exons. Consensus splice donor and acceptor sequences are present at all exon borders with the exception of the unusual splice donor at the end of exon VII. The 'invariable' GT dinucleotide in the consensus (C,A)AG/GTPuAGT is replaced by the exceptional GC. Based on 42 GC splice donor sequences identified by an extensive literature search we found a statistically highly significant better 'overall' match of the surrounding nucleotides to the consensus sequence compared to normal GT-sites. This confirms and extends the observation made recently by Jackson (Nucl. Acids Res., 19, 3795-3798 (1991)) derived from analysis of 26 cases. Analysis of ERCC6 cDNA clones revealed the occurrence of alternative polyadenylation, resulting in the (differential) expression of two mRNA molecules (which are barely detectable on Northern blots) of 5 and 7 kb in length.
Peptidomimetic inhibitors of Ras farnesylation and function in whole cells. (1993)

The ras protooncogene is involved in regulation of cell growth. Mutations that activate the protein result in uncontrolled cell growth. Ras undergoes a series of posttranslational processing events, the first of which, farnesylation, is crucial for the function of the protein. Inhibitors of the farnesyltransferase enzyme are therefore potential candidates for the development of anticancer drugs. Tetrapeptides have been reported to be good inhibitors of this enzyme in vitro. We have synthesized analogs of the tetrapeptide Cys-Val-Phe-Met by replacement of the amino-terminal amide bonds. One inhibitor, B581, is permeable to the cell membrane. In the cell, it inhibits processing of two farnesylated proteins, H-ras and lamin A, but it does not inhibit processing of a geranylgeranylated protein, Rap 1A. Microinjection of B581 into frog oocytes inhibits maturation induced by activated, farnesylated H-ras but not maturation induced by activated, geranylgeranylated H-ras or by progesterone. These results demonstrate that this peptide mimic inhibits farnesylation selectively in the cell. The inhibition of farnesylation results in inhibition of H-ras function.
Safety of Yttrium-90 Ibritumomab Tiuxetan Radioimmunotherapy for Relapsed Low-Grade, Follicular, or Transformed Non-Hodgkin’s Lymphoma (2003)

Single-dose (90)Y ibritumomab tiuxetan RIT has an acceptable safety profile in relapsed NHL patients with less than 25% lymphoma marrow involvement, adequate marrow reserve, platelets greater than 100,000 cells/ micro L, and neutrophils greater than 1,500 cells/ micro L.