gebhart/patrepeval huggingface dataset:
Chromatographic resolution of in vivo phosphorylated and nonphosphorylated eukaryotic translation initiation factor eIF-4E: increased cap affinity of the phosphorylated form. (1994)

Eukaryotic translation initiation factor eIF-4E plays a central role in the recognition of the 7-methylguanosine-containing cap structure of mRNA and the formation of initiation complexes during protein synthesis. eIF-4E exists in both phosphorylated and nonphosphorylated forms, and the primary site of phosphorylation has been identified. Previous studies have suggested that eIF-4E phosphorylation facilitates its participation in protein synthesis. However, the biochemical basis for the functional difference between the two forms of eIF-4E is unknown. To address this directly, we have developed a method for the separation of phosphorylated and nonphosphorylated eIF-4E from rabbit reticulocytes by chromatography on rRNA-Sepharose. Using the resultant purified forms, we have studied the protein's interaction with the cap analogs m7GTP and m7GpppG and with the cap of globin mRNA by fluorescence quenching of tryptophan residues. It was found that phosphorylated eIF-4E had 3- to 4-fold greater affinity for cap analogs and mRNA than nonphosphorylated eIF-4E. The equilibrium binding constants (x 10(5), expressed as M-1) for the interaction of phosphorylated eIF-4E with m7GTP, m7GpppG, and globin mRNA were 20.0 +/- 0.1, 16.4 +/- 0.1, and 31.0 +/- 0.1, respectively, whereas those for the nonphosphorylated form were 5.5 +/- 0.4, 4.3 +/- 0.4, and 10.0 +/- 0.1, respectively. Treatment with potato acid phosphatase converted the phosphorylated form to the nonphosphorylated form and decreased the binding constant for m7GTP by a factor of 3. The increased affinity for mRNA caps may account for the in vivo and in vitro correlations between eIF-4E phosphorylation and accelerated protein synthesis and cell growth.
248-nm DUV MoSiON embedded phase-shifting mask for 0.25 micrometer lithography (1995)

Over the past five years worldwide efforts have been made to develop new techniques for optical lithography enhancement. These techniques include optical proximity correction, off-axis illumination, pupil filtering and phase-shifting mask (PSM). Among many phase-shifting mask approaches, embedded PSM (EPSM) method has drawn significant interest due to its relatively simple reticle fabrication process and excellent lithographic performance, in particular, for dark field mask layers such as contact and via holes. Perhaps, the most difficult task in materializing the EPSM technology is the creation of a thin film structure that controls both phase and transmission. In addition, this film structure must withstand severe environment of mask making process and yet can be inspected and repaired successfully using currently available tool sets. The newly developed MoSiON material meets these requirements and has demonstrated a feasibility for DUV EPSM pilot production. In this paper, characteristics of the DUV lifetime test results. Details of reticle fabrication process including e-beam writing, dry etching, inspection and repair will be presented along with chemical durability data and process capability. Finally, wafer level lithographic performance for contact holes printed on a step-and-scan and a projection aligner will be shown to demonstrate lithographic performance of 248 nm DUV EPSM for 0.25 micrometer lithography.
Using neural nets to optimize retrieval in a fuzzy relational database (1992)

This paper examines the theory behind Fuzzy Sets and Back-Propagation Neural Nets, and how neural nets can be used to replace fuzzy sets and improve the query performance in a Fuzzy Relational Database (FRDB).Neural Nets and Fuzzy Relations are complementary theories that can be used together effectively in a Fuzzy Relational Database. This paper brings the theories of Fuzzy Sets, Neural Nets and Fuzzy Relational Databases together into one system.
Regenerative Proliferation in Organ Cultures of the Avian Cochlea: Identification of the Initial Progenitors and Determination of the Latency of the Proliferative Response (1996)

Sensory hair cells in the cochleae of birds are regenerated after the death of preexisting hair cells caused by acoustic over-stimulation or administration of ototoxic drugs. Regeneration involves renewed proliferation of cells in an epithelium that is otherwise mitotically quiescent. To determine the identity of the first cells that proliferate in response to the death of hair cells and to measure the latency of this proliferative response, we have studied hair-cell regeneration in organ culture. Cochleae from hatchling chicks were placed in culture, and hair cells were killed individually by a laser microbeam. The culture medium was then replaced with a medium that contained a labeled DNA precursor. The treated cochleae were incubated in the labeling media for different time periods before being fixed and processed for the visualization of proliferating cells. The first cells to initiate DNA replication in response to the death of hair cells were supporting cells within the cochlear sensory epithelium. All of the labeled supporting cells were located within 200 microns of the hair-cell lesions. These cells first entered S-phase approximately 16 hr after the death of hair cells. The results indicate that supporting cells are the precursors of regenerated hair cells and suggest that regenerative proliferation of supporting cells is triggered by signals that act locally within the damaged epithelium.
Protein Separations Using Colloidal Magnetic Nanoparticles (2003)

Phospholipid-coated colloidal magnetic nanoparticles with mean magnetite core size of 8 nm are shown to be effective ion exchange media for the recovery and separation of proteins from protein mixtures. These particles have high adsorptive capacities (up to 1200 mg protein/mL adsorbent, an order of magnitude larger than the best commercially available adsorbents) and exhibit none of the diffusional resistances offered by conventional porous ion exchange media. Protein-laden particles are readily recovered from the feed solution using high-gradient magnetic filtration.
An electrooptic analog-to-digital converter (1975)

A new type of analog-to-digital converter, the basic element of which is an array of electrooptic waveguide modulators, is described. Higher conversion rates and simplicity are potential advantages in comparison with conventional electronic conversion techniques.
Black Conductive Titanium Oxide High-Capacity Materials for Battery Electrodes (2011)

Stoichiometric titanium dioxide (TiO{sub 2}) is one of the most widely studied transitionmetal oxides because of its many potential applications in photoelectrochemical systems, such as dye-sensitized TiO{sub 2} electrodes for photovoltaic solar cells, and water-splitting catalysts for hydrogen generation, and in environmental purification for creating or degrading specific compounds. However, TiO{sub 2} has a wide bandgap and high electrical resistivity, which limits its use as an electrode. A set of non-stoichiometric titanium oxides called the Magneli phases, having a general formula of Ti{sub n}O{sub 2n-1} with n between 4 and 10, exhibits lower bandgaps and resistivities, with the highest electrical conductivities reported for Ti{sub 4}O{sub 7}. These phases have been formulated under different conditions, but in all reported cases the resulting oxides have minimum grain sizes on the order of micrometers, regardless of the size of the starting titanium compounds. In this method, nanoparticles of TiO{sub 2} or hydrogen titanates are first coated with carbon using either wet or dry chemistry methods. During this process the size and shape of the nanoparticles are 'locked in.' Subsequently the carbon-coated nanoparticles are heated. This results in the transformation of the original TiO{sub 2} or hydrogen titanates to Magneli phases without coarsening, so that the original size and shape of the nanoparticles are maintained to a precise degree. People who work on batteries, fuel cells, ultracapacitors, electrosynthesis cells, electro-chemical devices, and soil remediation have applications that could benefit from using nanoscale Magneli phases of titanium oxide. Application of these electrode materials may not be limited to substitution for TiO{sub 2} electrodes. Combining the robustness and photosensitivity of TiO{sub 2} with higher electrical conductivity may result in a general electrode material.
Arachidonic acid to eicosapentaenoic acid ratio in blood correlates positively with clinical symptoms of depression (1996)

In this study of 20 moderately to severely depressed patients, diagnosed using current research diagnostic criteria and excluding known bipolar affective disorder and reactive depression, we investigated relationships between severity of depression and levels and ratios of n-3 and n-6 long-chain polyunsaturated fatty acids (PUFA) in plasma and erythrocyte phospholipids (PL). Severity of depression was measured using the 21-item Hamilton depression rating scale (HRS) and a second linear rating scale (LRS) of severity of depressive symptoms that omitted anxiety symptoms. There was a significant correlation between the ratio of erythrocyte PL arachidonic acid (AA) to eicosapentaenoic acid (EPA) and severity of depression as rated by the HRS (P < 0.05) and the LRS for depression (P < 0.01). There was also a significant negative correlation between erythrocyte EPA and the LRS (P < 0.05). The AA/EPA ratio in plasma PL and the ratio of erythrocyte long-chain (C20 and C22 carbon) n-6 to long-chain n-3 PUFA were also significantly correlated with the LRS (P < 0.05). These findings do not appear to be simply explained by differences in dietary intake of EPA. We cannot determine whether the high ratios of AA/EPA in both plasma and erythrocyte PL are the result of depression or whether tissue PUFA change predate the depressive symptoms. We suggest, however, that our findings provide a basis for studying the effect of the nutritional supplementation of depressed subjects, aimed at reducing the AA/EPA ratio in tissues and severity of depression.
Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors (2016)

Receptor tyrosine kinases (RTKs) are key molecules in numerous cellular processes, the inhibitors of which play an important role in the clinic. Among them are the vascular endothelial growth factor (VEGF) family members and their receptors (VEGFR), which are essential in the formation of new blood vessels by angiogenesis. Anti-VEGF therapy has already shown promising results in oncology and ophthalmology, but one of the challenges in the field is the design of specific small-molecule inhibitors for these receptors. We show the identification and characterization of small 6-mer peptides that target the extracellular ligand-binding domain of all three VEGF receptors. These peptides specifically prevent the binding of VEGF family members to all three receptors and downstream signaling but do not affect other angiogenic RTKs and their ligands. One of the selected peptides was also very effective at preventing pathological angiogenesis in a mouse model of retinopathy, normalizing the vasculature to levels similar to those of a normal developing retina. Collectively, our results suggest that these peptides are pan-VEGF inhibitors directed at a common binding pocket shared by all three VEGFRs. These peptides and the druggable binding site they target might be important for the development of novel and selective small-molecule, extracellular ligand-binding inhibitors of RTKs (eTKIs) for angiogenic-dependent diseases.
Heterobimetallic Catalysis in Asymmetric 1,4‐Addition of O‐Alkylhydroxylamine to Enones. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Lysophospholipids Enhance Matrix Metalloproteinase-2 Expression in Human Endothelial Cells (2005)

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are both low-molecular-weight lysophospholipids, which promote cell proliferation, migration, and invasion via interaction with a family of specific G protein-coupled receptors. Matrix metalloproteinases (MMPs) are zinc-dependent proteolytic enzymes, which are involved in degradation of the extracellular matrix and play critical roles in endothelial cell migration and matrix remodeling during angiogenesis. Among these MMPs, MMP-2 is known to trigger cell migration. In our present study, we examined the effects of LPA and S1P on MMP-2 expression in human endothelial cells. We showed that LPA and S1P enhanced MMP-2 expression in mRNA, protein levels, and also enzymatic activity of cells of the EAhy926 human endothelial cell line. The enhancement effects occurred in concentration- and time-dependent manners. Results from real-time PCR, Western blots, and substrate gels indicated that these enhancement effects were mediated through MAPK kinase/ERK-, nuclear factor-kappaB-, and calcium influx-dependent pathways. Furthermore, we show that endothelial cell invasion of the gel was enhanced by lysophospholipids, and the induction could be prevented by an MMP inhibitor, GM6001. These observations suggest that LPA and S1P may play important roles in endothelial cell invasion by regulating the expression of MMP-2.
Photoelectric effect from a metal surface: a revisited theoretical model (1992)

The Sommerfeld model extended to include radiation–electron interaction in the regime of highly intense fields is taken as the basis for studying theoretically the laser multiphoton photoelectric effect from a metal surface. Numerical analysis is carried out without approximations other than those inherent in the model itself; the study of the multiphoton aspect of the problem is based on a scheme that is nonperturbative in an essential way. The numerical analysis facilitates insight into the potential and the limits of the model in the interpretation of recent experiments and into the similarities and differences between the metal multiphoton effect and atomic multiphoton ionization. The results obtained and the importance of the competing processes not considered here clearly call for more realistic models to account for recent laboratory observations.
Use of magnesium cation in aldol additions - a convenient method for achieving anti-aldol selectivity (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of magnesium cation in aldol additions - a convenient method for achieving anti-aldol selectivityKevin A. Swiss, Woo Baeg Choi, Dennis C. Liotta, Ahmed F. Abdel-Magid, and Cynthia A. MaryanoffCite this: J. Org. Chem. 1991, 56, 21, 5978–5980Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://pubs.acs.org/doi/10.1021/jo00021a004https://doi.org/10.1021/jo00021a004research-articleACS PublicationsRequest reuse permissionsArticle Views733Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The formation and maintenance of the definitive endoderm lineage in the mouse: involvement of HNF3/forkhead proteins (1993)

Little is known about genes that govern the development of the definitive endoderm in mammals; this germ layer gives rise to the intestinal epithelium and various other cell types, such as hepatocytes, derived from the gut. The discovery that the rat hepatocyte transcription factor HNF3 is similar to the Drosophila forkhead gene, which plays a critical role in gut development in the fly, led us to isolate genes containing the HNF3/forkhead (HFH) domain that are expressed in mouse endoderm development. We recovered mouse HNF3 beta from an embryo cDNA library and found that the gene is first expressed in the anterior portion of the primitive streak at the onset of gastrulation, in a region where definitive endoderm first arises. Its expression persists in axial structures derived from the mouse equivalent of Hensen's node, namely definitive endoderm and notochord, and in the ventral region of the developing neural tube. Expression of the highly related gene, HNF3 alpha, appears to initiate later than HNF3 beta and is first seen in midline endoderm cells. Expression subsequently appears in notochord, ventral neural tube, and gut endoderm in patterns similar to HNF3 beta. Microscale DNA binding assays show that HNF3 proteins are detectable in the midgut at 9.5 days p.c. At later stages HNF3 mRNAs and protein are expressed strongly in endoderm-derived tissues such as the liver. HNF3 is also the only known hepatocyte-enriched transcription factor present in a highly de-differentiated liver cell line that retains the capacity to redifferentiate to the hepatic phenotype. Taken together, these studies suggest that HNF3 alpha and HNF3 beta are involved in both the initiation and maintenance of the endodermal lineage. We also discovered a novel HFH-containing gene, HFH-E5.1, that is expressed transiently in posterior ectoderm and mesoderm at the primitive streak stage, and later predominantly in the neural tube. HFH-E5.1 is highly similar in structure and expression profile to the Drosophila HFH gene FD4, suggesting that HFH family members have different, evolutionarily conserved roles in development.
Recursive Deep Models for Semantic Compositionality Over a Sentiment Treebank (2013)

Richard Socher, Alex Perelygin, Jean Wu, Jason Chuang, Christopher D. Manning, Andrew Ng, Christopher Potts. Proceedings of the 2013 Conference on Empirical Methods in Natural Language Processing. 2013.
Postoperative Intravenous Ferric Carboxymaltose Reduces Transfusion Amounts after Orthopedic Hip Surgery (2018)

Orthopedic hip surgery is associated with considerable blood loss and high rates of perioperative transfusion. 1) A recent meta-analysis of 19 studies showed that 24% of patients undergoing total hip replacement or total knee replacement and 44% of those with hip fracture had preoperative anemia. It has also been reported that more than 50% of such patients became anemic after surgery due to intraoperative blood loss. 2,3) Allogenic red blood cell transfusion is frequently used to treat acute postoperative anemia, and 20%-50% of these patients receive at least one unit of red blood cells. In this respect, the three-pillar concept of patient blood management was developed to reduce the risk of transfusion. n addition to perioperative blood loss, iron homeostasis can result in postoperative anemia through a condition called functional iron deficiency, which is caused
Neurodevelopmental Outcome of Extremely Low Birth Weight Infants from the Vermont Oxford Network: 1998–2003 (2009)

ELBW infants completing evaluation were at a high risk for severe disability. There are considerable differences among participating centers in attrition at follow-up. Further resources will be needed to study the effect of follow-up care for this group of infants.
Limits on super-resolution and how to break them (2002)

Nearly all super-resolution algorithms are based on the fundamental constraints that the super-resolution image should generate low resolution input images when appropriately warped and down-sampled to model the image formation process. (These reconstruction constraints are normally combined with some form of smoothness prior to regularize their solution.) We derive a sequence of analytical results which show that the reconstruction constraints provide less and less useful information as the magnification factor increases. We also validate these results empirically and show that, for large enough magnification factors, any smoothness prior leads to overly smooth results with very little high-frequency content. Next, we propose a super-resolution algorithm that uses a different kind of constraint in addition to the reconstruction constraints. The algorithm attempts to recognize local features in the low-resolution images and then enhances their resolution in an appropriate manner. We call such a super-resolution algorithm a hallucination or reconstruction algorithm. We tried our hallucination algorithm on two different data sets, frontal images of faces and printed Roman text. We obtained significantly better results than existing reconstruction-based algorithms, both qualitatively and in terms of RMS pixel error.
Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. (1985)

A recombinant phage that carries the cytoplasmic beta-actin gene was isolated from a human DNA library. The nucleotide sequence of this gene was determined. The amino acid sequence deduced from the nucleotide sequence matches perfectly that of beta-actin from human fibroblasts. The gene contains five introns. A large intron was found in the 5' untranslated region six nucleotides upstream from the ATG initiation codon. Four introns were found within the coding region at codons specifying amino acids 41/42, 121/122, 267, and 327/328. In contrast to the human cardiac muscle actin gene, the aorta-type smooth muscle actin gene, and the stomach-type smooth muscle actin gene, the beta-actin gene lacks the codon for cysteine between the ATG initiation codon and the codon for the NH2-terminal amino acid of the mature protein. Hybridization of genomic DNA with DNA fragments derived from intron I in the 5' untranslated region and from intron III strongly suggests the presence of a single beta-actin gene in the human genome. The DNA sequences of the coding region, of the 3' untranslated region, and of the sequence block between the "CCAAT" box and "TATA" box in the 5' flanking DNA of the human beta-actin gene are highly homologous to the corresponding sequences of the rat and chicken beta-actin genes. Unexpectedly, the sequence of intron III of the human beta-actin gene shows considerable homology to that of the rat beta-actin gene.