gebhart/patrepeval huggingface dataset:
Constitutive expression of recombinant proteins in the methylotrophic yeastHansenula polymorpha using thePMA1 promoter (2000)

The methylotrophic yeast H. polymorpha is a popular system for the expression of recombinant proteins using the strong and regulatable methanol oxidase (MOX) promoter. Here we show that the constitutive PMA1 promoter can programme the expression of two heterologous proteins, glucose oxidase and human serum albumin. A constitutive promoter provides a useful additional facility to the H. polymorpha expression system because it allows a simplified fermentation regime, avoids the use of methanol, which is both toxic and an explosive hazard, and allows more flexibility for ectopic gene expression during the course of academic studies. A fragment previously isolated in a promoter screen, using glucose oxidase (GOD) as a reporter gene, was shown to consist of the promoter region and the first 659 bp of the H. polymorpha PMA1 gene, encoding the plasma membrane H(+)-ATPase. When the PMA1 promoter was optimally aligned with the GOD coding region, it produced 185 mg/l glucose oxidase in high cell density fed batch fermentations, whereas in previous experiments using the MOX promoter, a yield of 500 mg/l was recovered. The PMA1 promoter was also used to express recombinant human serum albumin (rHA) in H. polymorpha. In high cell density fermentations the PMA1 promoter produced 460 mg/l rHA, whereas 280 mg/l rHA was obtained using the MOX promoter. Taken together, these experiments show that the HpPMA1 programmes the constitutive expression of recombinant proteins and provides a yield comparable to that from the MOX promoter.
Mobility management in wireless ATM networks (1997)

Mobile ATM offers a common wired network infrastructure to support mobility of wireless terminals, independent of the wireless access protocol. In addition, it allows seamless migration to future wireless broadband services, such as wireless ATM, by enabling mobility of end-to-end ATM connections. In spite of the diversity in mobile networking technologies (e.g., cellular telephony, mobile-IP, packet data services, PCS), all of them require two fundamental mechanisms: location management and handoff. This article describes different schemes for augmenting a wired ATM network to support location management of mobile terminals and handoff protocols for rerouting a connection data path when the endpoint moves. A prototype implementation of mobile ATM integrating mobility support with ATM signaling and connection setup, is presented. It shows how mobile ATM may be used to provide mobility support to an IP terminal using non-ATM wireless access.
Water harvest via dewing (2012)

Harvesting water from humid air via dewing can provide a viable solution to a water shortage problem where liquid-phase water is not available. Here we experimentally quantify the effects of wettability and geometry of the condensation substrate on the water harvest efficiency. Uniformly hydrophilic surfaces are found to exhibit higher rates of water condensation and collection than surfaces with lower wettability. This is in contrast to a fog basking method where the most efficient surface consists of hydrophilic islands surrounded by hydrophobic background. A thin drainage path in the lower portion of the condensation substrate is revealed to greatly enhance the water collection efficiency. The optimal surface conditions found in this work can be used to design a practical device that harvests water as its biological counterpart, a green tree frog, Litoria caerulea , does during the dry season in tropical northern Australia.
SOI MOSFET on low cost SPIMOX substrate (2002)

Separation by plasma implantation of oxygen (SPIMOX) is a low-cost, high-throughput process for fabricating silicon on insulator (SOI) substrates. MOSFETs are fabricated on SPIMOX substrates and their characteristics are reported for the first time. The transistor characteristics and effective electron mobility of the silicon layer are compared to that in commercial separation by implantation of oxygen (SIMOX) wafers. The effect of the back gate on the channel, which is unique to SOI, is observed. The gate oxides and surface roughness of SPIMOX wafers are also studied.
Effects of meclofenoxate and Extr. Rhodiolae roseae L. on electroconvulsive shock-impaired learning and memory in rats. (1986)

In experiments on albino rats, the authors studied the effects of meclofenoxate and Extr. Rhodiolae roseae on the memory-impairing action of convulsant electroshock. "Step-down" passive avoidance training with negative reinforcement was used to trace the changes in memory. Meclofenoxate administered i.p. in a dose of 100 mg/kg body weight for five days prevented the retrograde amnesia observed after convulsant electroshock upon retention testing on the 3rd and 24th hr after the end of the training session. The Rhodiola extract administered orally in a dose of 0.10 ml/rat for 10 days, which in other experimental approaches improved learning and memory, remained ineffective here. The role of biogenic monoamines in the learning- and memory-improving effects of meclofenoxate is considered on the basis of earlier studies by the authors.
Potential Barriers in Management of Refurbishment Projects (2005)

The study presents the sources of the barriers in management of refurbishment projects that may cause delays and escalate costs. To achieve the study objectives, the information was acquired through personal interviews and in-depth discussions with the architects and contractors of the seven refurbishment projects selected in eastern province of Saudi Arabia. It addresses issues often overlooked by the management team in a refurbishment project during the design, document preparation, and construction stages. The twelve most important sources of barriers were the degradation of undamaged materials over time, formidable variations to original structure, salvaged materials, conflict between responsibilities and liabilities, missing design information, illogical imposed conditions, selecting contract types, improper site survey, concurrent operation by owners, selection of contractor, escalated costs and delays during refurbishment, and underpayment for architect’s services were revealed from the in-depth interviews and discussion with the architects and the contractors of the refurbishment projects. Reasons for these problems of management are reviewed with emphasis on the role of the architect in avoiding underestimation of required work during the stages of the project. Recommendations are given to minimize ambiguities in the scope of the work and avoid disputes among the parties to the contract. This can be achieved by employing an experienced architect and by making sound prompt decisions through out the project lifecycle.
Campylobacter infections (1993)

Campylobacter jejuni (and also Campylobacter coli) are currently thought to be the number one cause of sporadic bacterial gastrointestinal infection in the United States. Complications do occur, although infrequently, and recent studies suggest a strong association of Campylobacter infection with Guillain-Barré syndrome. There are no specific clinical findings that differentiate Campylobacter species from other causes of infection, and diagnosis is dependent on laboratory testing. There are still many clinical laboratories that do not routinely look for C. jejuni in routine stool cultures as should be standard practice. New species of Campylobacter associated with gastrointestinal infections have been recently identified that may not be recovered by methods used for the more common C. jejuni. Clinical studies on antimicrobial therapy of Campylobacter infections show variable effects on symptoms but ensure microbiologic cure. New studies suggest that development of quinolone resistance during therapy is common with Campylobacter species, which may limit quinolone therapy in the future.
Hydrolysed microspheres from cross-linked polymethyl methacrylate (Hydrogel). A new embolic material for interventional neuroradiology. (1991)

Highly hydrophilic, perfectly smooth and spherical microspheres have been synthetized. These non-biodegradable microspheres absorb water in varying degrees and can be injected easily through microcatheters due to their slippery and compressible characteristics. The material was successfully used for embolization of 4 vascular intracranial tumours and 2 spinal vascular lesions in the cervical region, by superselective delivery. Histopathology confirmed absolute inertness of the microspheres.
Splicing of Effector Cell Protease Receptor-1 mRNA Is Modulated by an Unusual Retained Intron (1994)

Effector cell protease receptor-1 (EPR-1) is a transmembrane glycoprotein receptor for factor Xa that contributes to cell surface assembly of proteolytic activities and leukocyte mitogenesis. It is now shown that membrane expression of EPR-1 is dynamically modulated by mRNA splicing. Northern hybridization analysis of EPR-1-expressing cells and genetically engineered transfectants demonstrates that this mechanism involves removal of a 451 bp intervening sequence retained in 70-90% of mature mRNA, as quantitated by polymerase chain reaction amplification and ribonuclease protection studies. Splicing of the intervening sequence occurs in a cell type-specific fashion, as judged by the constitutive membrane overexpression of EPR-1 in certain leukemic B lymphocytes and monocytic cells. Furthermore, phenotypic analysis of cell lines stably transfected with functionally spliced or unspliced EPR-1 constructs suggests a potential role of intron cis-acting sequence(s) in splicing regulation. Instead of a transmembrane receptor for factor Xa (EPR-1a), the most prevalent unspliced EPR-1 transcript generates a novel truncated protein of 110 amino acids (EPR-1b), in which a unique intron-encoded -COOH terminus carries a potential nuclear targeting signal PPQHRAKS. An antibody generated against the intron-encoded sequence of EPR-1b demonstrates prominent nuclear localization of this variant isoform in indirect immunofluorescence staining of permeabilized cells. These findings provide evidence for a novel mechanism based on high efficiency intron retention modulating factor Xa-dependent cellular effector functions.
beta-Amyloid precursor protein binds to the neurite-promoting IKVAV site of laminin. (1993)

We previously characterized a 110-kDa membrane-associated laminin-binding protein (LBP110) from brain which binds the laminin A chain -Ile-Lys-Val-Ala-Val-(IKVAV) site and increases in injury. Here we demonstrate that antisera directed against different epitopes of beta-amyloid precursor protein (APP) recognize LBP110 and that APP is recognized by LBP110 antiserum. APP specifically binds IKVAV and not another biologically active laminin-derived peptide containing the amino acid sequence -Tyr-Ile-Gly-Ser-Arg-. PC-12 cells transfected with antisense APP RNA produce less APP and LBP110, and they form fewer processes when cultured on either laminin or the IKVAV peptide. Thus, LBP110 is a member of the APP family and a function for APP in neurite outgrowth is now defined.
Age and Gender Estimation of Unfiltered Faces (2014)

This paper concerns the estimation of facial attributes-namely, age and gender-from images of faces acquired in challenging, in the wild conditions. This problem has received far less attention than the related problem of face recognition, and in particular, has not enjoyed the same dramatic improvement in capabilities demonstrated by contemporary face recognition systems. Here, we address this problem by making the following contributions. First, in answer to one of the key problems of age estimation research-absence of data-we offer a unique data set of face images, labeled for age and gender, acquired by smart-phones and other mobile devices, and uploaded without manual filtering to online image repositories. We show the images in our collection to be more challenging than those offered by other face-photo benchmarks. Second, we describe the dropout-support vector machine approach used by our system for face attribute estimation, in order to avoid over-fitting. This method, inspired by the dropout learning techniques now popular with deep belief networks, is applied here for training support vector machines, to the best of our knowledge, for the first time. Finally, we present a robust face alignment technique, which explicitly considers the uncertainties of facial feature detectors. We report extensive tests analyzing both the difficulty levels of contemporary benchmarks as well as the capabilities of our own system. These show our method to outperform state-of-the-art by a wide margin.
Biochemical and Pharmacological Characterization of [3H]GBR 12935 Binding In Vitro to Rat Striatal Membranes: Labeling of the Dopamine Uptake Complex (1987)

Binding of the selective dopamine (DA) uptake inhibitor [3H]GBR 12935 to rat striatal membranes was characterized biochemically and pharmacologically. [3H]GBR 12935 binding at 0 degree C was reversible and saturable and Scatchard analysis indicated a single binding site with a KD of 5.5 nM and a Bmax of 760 pmol/mg tissue. [3H]GBR 12935 labeled two binding sites. One binding site was identified as the classic DA uptake site, since methylphenidate, cocaine, diclofensine, and Lu 19-005 potently inhibited [3H]GBR 12935 binding to it. Binding to the second site was inhibited by high concentrations of the above compounds. IC50 values for inhibition of [3H]GBR 12935 binding to the DA uptake site were proportional to IC50 values for inhibition of DA uptake. However, substrates of DA uptake, e.g., DA and 1-methyl-4-phenylpyridine, and DA releasers, e.g., the amphetamines, inhibited [3H]GBR 12935 binding less than DA uptake. Rate experiments excluded the possibility that these "weak" inhibitors affected the binding by allosteric coupled binding sites. The second binding site was not a noradrenergic, serotonergic, or GABAergic uptake site. Neither was it a dopaminergic, acetylcholinergic, histaminic, serotonergic, or adrenergic receptor. However, [3H]GBR 12935 was potently displaced from it by disubstituted piperazine derivatives, i.e., flupentixol and piflutixol. DA uptake and the DA uptake binding site of [3H]GBR 12935 were located primarily in the striatum, but the piperazine acceptor site was distributed uniformly throughout the brain. Also only the DA uptake binding site was destroyed by 6-OH-DA. Thus, [3H]GBR 12935 labels the classic DA uptake site in rat striatum and also a piperazine acceptor site. Substrates for DA uptake and releasers of DA inhibited [3H]GBR 12935 binding with low potency, but did not alter the rate constants for [3H]GBR 12935 binding. Therefore inhibitors of DA uptake label the carrier site and prevent the carrier process.
Crystal and Molecular Structure of cyclo-L-Aspartyl-L-alanyl (3,6-Dioxo-5-methyl-2-piperazineacetic acid). (1987)

Le compose du titre cristallise dans le groupe d'espace P2 1 . Affinement de la structure jusqu'a R=0,032. Le cycle piperazine est plan, avec une legere tendance vers la conformation bâteau avec les 2 substituants quasi axiaux. La chaine aspartyl est repliee sur le cycle
Management of renovascular disease: a review of renal artery stenting in ten studies (1999)

To evaluate the efficacy and safety of renal artery stents in renovascular disease, we identified 10 descriptive studies containing sufficient information for systematic evaluation. No randomized comparisons of stenting with angioplasty or with surgery were found. Overall, stents were placed in 416 renal arteries in 379 patients, mean age 64 years (range 27-84), 56% male. Of the stenoses, 97% were atheromatous (inter-study range 71-100%), 80% ostial (22-100%) and 31% bilateral (12-87%). The clinical indication for stenting was usually hypertension with or without mild renal impairment. Radiological indications for stenting were: narrowing of > or = 50% (in 9/10 studies) as a result of elastic recoil (58%) or dissection (2%) at the time of angioplasty; restenosis some time after angioplasty (15%); or as a primary procedure (25%). Technical success was reported in 96-100% of procedures. Restenosis (> or = 50% narrowing), evaluated in 312/416 (75%) arteries, generally between 6 and 12 months, was 16% overall. Hypertension was cured by stenting (DBP < or = 90 mmHg on no treatment) in 34/379 (9%) overall and in 34/207 (16%) of those whose renal function was normal initially. Six of 379 (1.6%) patients died within 30 days of stenting, but in only two (0.5%) was death judged to be procedure-related. Complications, other than those which led to dialysis, occurred in 42/379 (13%) patients, one third requiring intervention, ranging from blood transfusion to a surgical bypass procedure. Renal function as judged by serum creatinine concentration (SCC) improved in 26%, stabilized in 48% and deteriorated in 26% of patients whose renal function was impaired initially (SCC > 133 mumol/l). In one study, with average baseline SCC > 200 mumol/l, successful stenting slowed the rate of progression of renal failure when renal function was deteriorating beforehand. Nine of 379 (2.4%) patients, including 7/14 (50%) whose SCC was > or = 400 mumol/l initially, required dialysis after stenting. Stenting should be offered by specialist centres as a secondary procedure for unsuccessful angioplasty, or restenosis following angioplasty, to patients with renovascular disease and uncontrolled hypertension, advancing renal failure or pulmonary oedema.
Cutting Edge: IL-2 Is Critically Required for the In Vitro Activation of CD4+CD25+ T Cell Suppressor Function (2004)

CD4(+)CD25(+) T cells are potent immunoregulatory cells that suppress TCR-induced proliferation of CD4 and CD8 T cells in vitro by a cell contact-dependent mechanism. Addition of IL-2 or anti-CD28 abrogates CD4(+)CD25(+)-mediated suppression of proliferation and has been assumed to "break suppression." We examined IL-2 mRNA by quantitative PCR in cocultures of mouse CD4(+)CD25(+) and CD4(+)CD25(-) T cells. Although IL-2 gene transcription was inhibited in the presence or absence of exogenous IL-2, the addition of anti-CD28 stimulated endogenous IL-2 production. Surprisingly, transcription of IL-2 mRNA was also restored in the cocultures in the presence of anti-IL-2. These results are most compatible with a model in which CD4(+)CD25(+) T cells do not suppress the initial activation of CD4(+)CD25(-) T cells, but mediate their suppressive effects following production of IL-2 by the responder cells resulting in both the expansion of the CD4(+)CD25(+) T cells and the induction of their suppressor function.
Gastrointestinal Tract as a Major Site of CD4 + T Cell Depletion and Viral Replication in SIV Infection (1998)

Human and simian immunodeficiency virus (HIV and SIV) replicate optimally in activated memory CD4(+) T cells, a cell type that is abundant in the intestine. SIV infection of rhesus monkeys resulted in profound and selective depletion of CD4+ T cells in the intestine within days of infection, before any such changes in peripheral lymphoid tissues. The loss of CD4+ T cells in the intestine occurred coincident with productive infection of large numbers of mononuclear cells at this site. The intestine appears to be a major target for SIV replication and the major site of CD4+ T cell loss in early SIV infection.
Amyloid β-peptide inhibition of the PKA/CREB pathway and long-term potentiation: Reversibility by drugs that enhance cAMP signaling (2002)

Changes in hippocampal function seem critical for cognitive impairment in Alzheimer's disease (AD). Although there is eventual loss of synapses in both AD and animal models of AD, deficits in spatial memory and inhibition of long-term potentiation (LTP) precede morphological alterations in the models, suggesting earlier biochemical changes in the disease. In the studies reported here we demonstrate that amyloid beta-peptide (Abeta) treatment of cultured hippocampal neurons leads to the inactivation of protein kinase A (PKA) and persistence of its regulatory subunit PKAIIalpha. Consistent with this, CREB phosphorylation in response to glutamate is decreased, and the decrease is reversed by rolipram, a phosphodiesterase inhibitor that raises cAMP and leads to the dissociation of the PKA catalytic and regulatory subunits. It is likely that a similar mechanism underlies Alphabeta inhibition of LTP, because rolipram and forskolin, agents that enhance the cAMP-signaling pathway, can reverse this inhibition. This reversal is blocked by H89, an inhibitor of PKA. These observations suggest that Alphabeta acts directly on the pathways involved in the formation of late LTP and agents that enhance the cAMP/PKA/CREB-signaling pathway have potential for the treatment of AD.
CRYSTALSversion 12: software for guided crystal structure analysis (2003)

The determination of small-molecule structures from single-crystal\nX-ray data is being carried out by researchers with little or no crys-\ntallographic training. At the same time, completely automatic crystal\nstructure analysis can still only be achieved under very favourable\nconditions. Many of the problems that cause automatic systems to fail\ncould be resolved with suitable chemical insight, and until this is built-\nin, programs continue to need human guidance. CRYSTALS version\n12 contains a modern crystallographic human-interface design, and\nnovel strategies incorporating chemical knowledge and sensible\ncrystallographic guidance into crystal structure analysis software.
[Experience with the use of ozone for the treatment of chronic viral hepatitis]. (2010)

Comparative data are presented illustrating efficiency of the treatment of patients with chronic viral hepatitis by standard therapeutic methods and basal therapy combined with ozone therapy. Patients treated with the use of ozone exhibited better biochemical, virological, and functional characteristics compared with their counterparts managed by standard methods. The results of the study suggest a higher efficiency of combined therapy and reduced risk of development of cholelithiasis.
A simple, real-time range camera (2003)

A simple imaging range sensor is described, based on the measurement of focal error, as described by A. Pentland (1982 and 1987). The current implementation can produce range over a 1 m/sup 3/ workspace with a measured standard error of 2.5% (4.5 significant bits of data). The system is implemented using relatively inexpensive commercial image-processing equipment. Experience shows that this ranging technique can be both economical and practical for tasks which require quick and reliable but coarse estimates of range. Examples of such tasks are initial target acquisition or obtaining the initial coarse estimate of stereo disparity in a coarse-to-fine stereo algorithm.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
EFFECT OF RANIBIZUMAB RETREATMENT FREQUENCY ON NEUROSENSORY RETINAL VOLUME IN NEOVASCULAR AMD (2009)

Patients receiving fewer injections of ranibizumab appeared less likely to maintain anatomic improvements achieved following commencement of ranibizumab therapy; regression of these improvements was associated with deterioration in visual acuity.
Lee distance Gray codes (2002)

Presents 4 methods of generating a Lee distance Gray code. The first two methods presented are for radix k numbers, and the other two methods are for mixed radix numbers.