gebhart/patrepeval huggingface dataset:
A generalized hidden Markov model with state-conditioned trend functions of time for the speech signal (1992)

Abstract The standard hidden Markov model (HMM) and the hidden filter model assume local or state-conditioned stationarity for the modeled signal. In this work we generalize these models and develop the ‘trended HMM’ to allow the local, as well as the global (via a Markov chain), non-stationarity to be represented in the model. The mathematical structure of the trended HMM can be described by a discrete-time Markov process with its states associated with distinct regression functions on time, or alternatively by a ‘deterministic trend plus stationary residual’ time series with its parameters governed by the evolution of a Markov chain. The EM algorithm is applied to obtain closed-form re-estimation formulas for the model parameters. Compared with the types of HMMs developed in the past, the trended HMM is a more faithful and more structured representation of many classes of speech sounds whose production involves strong articulatory dynamics. As such, it is expected to be a more suitable model for use in speech processing applications.
Differential inhibition of T and B cell function in IL-4-dependent IgE production by cyclosporin A and methylprednisolone. (1990)

The present study examines the role of the immunosuppressive agents methylprednisolone (MPN) and cyclosporin (Cs)A on IL-4-dependent IgE and IgG production. Addition of optimal amounts of IL-4 (100 U/ml) to cultures of tonsil mononuclear cells resulted in a mean increase in IgG production of 175% and in IgE production of 2460%. Frequency analysis of IgE- and IgG-producing B cells, using an ELISA spot assay, showed parallel increases in both Ig production and numbers of Ig-secreting B cells. IgE production was also enhanced by addition of IL-2 (10 U/ml) and maximal IgE production was obtained with a combination of IL-4 and IL-2. MPN (10(-7) M) and CsA (1 microgram/ml) markedly reduced IL4-induced IgE and IgG production as well as numbers of Ig-secreting cells in a dose-dependent fashion. The suppression of Ig production by the cyclosporins was restricted to the immunosuppressive compounds CsA, CsG, and dihydro-CsD, but not the nonimmunosuppressive drug CsH. Delayed addition of CsA revealed that inhibition was maximal when the drug was added during the first 48 h after addition of IL-4 to the culture. Addition of IL-2 (10 U/ml) partially overcame the inhibition induced by CsA. In coculture experiments, in which separated T or B cells were precultured with the drugs and the cells were then combined and further incubated in the presence of IL-4, the suppressive effects of CsA on IgE production were related to pretreatment of the T but not B cells. The maximum inhibiting effects of MPN were similarly observed when the drug was present in the cultures from the beginning, and addition of IL-2 also partially reversed this inhibition. In contrast to the results with CsA, pretreatment of the B but not T cells with MPN-reduced IgE production. These studies demonstrate that IL-4 increases both numbers of IgE-secreting cells as well as IgE production and CsA and MPN differentially affect the responding T and B cells, resulting in inhibition of Ig production.
Fluorometric Detection of MicroRNA Using Isothermal Gene Amplification and Graphene Oxide (2016)

We have developed a facile fluorometric system for the detection of microRNA (miRNA), using rolling circle amplification (RCA), graphene oxide (GO), and fluorescently labeled peptide nucleic acid (F-PNA). The padlock probe DNA complementary to a target miRNA was selectively ligated to form circular DNA that was then used as the template for RCA. F-PNAs complementary to the target miRNA were annealed to multiple sites of the isothermally amplified single-stranded RCA product (RCAP) containing multiple target miRNA sequences. This F-PNA/RCAP duplex is less adsorbed onto the GO monolayer, thus attenuating the quenching of F-PNA fluorescence by GO. In the absence of target miRNA (and hence the absence of RCA and duplex formation), the free F-PNA is completely adsorbed onto the GO monolayer and fluorescence quenching ensues. Thus, GO-based fluorescence detection coupled with isothermal gene amplification would be a simple and convenient method for the quantitative detection of miRNA.
Regeneration of fat cells from myofibroblasts during wound healing (2017)

Although regeneration through the reprogramming of one cell lineage to another occurs in fish and amphibians, it has not been observed in mammals. We discovered in the mouse that during wound healing, adipocytes regenerate from myofibroblasts, a cell type thought to be differentiated and nonadipogenic. Myofibroblast reprogramming required neogenic hair follicles, which triggered bone morphogenetic protein (BMP) signaling and then activation of adipocyte transcription factors expressed during development. Overexpression of the BMP antagonist Noggin in hair follicles or deletion of the BMP receptor in myofibroblasts prevented adipocyte formation. Adipocytes formed from human keloid fibroblasts either when treated with BMP or when placed with human hair follicles in vitro<i>.</i> Thus, we identify the myofibroblast as a plastic cell type that may be manipulated to treat scars in humans.
Actin stress fibers transmit and focus force to activate mechanosensitive channels (2008)

Mechanosensitive (MS) channels are expressed in various cells in a wide range of phylogenetic lineages from bacteria to humans. Understanding the molecular and biophysical mechanisms of their activation is an important research pursuit. It is controversial whether eukaryotic MS channels need accessory proteins -- typically cytoskeletal structures -- for activation, because MS channel activities are modulated by pharmacological treatments that affect the cytoskeleton. Here we demonstrate that direct mechanical stimulation (stretching) of an actin stress fiber using optical tweezers can activate MS channels in cultured human umbilical vein endothelial cells (HUVECs). Furthermore, by using high-speed total internal reflection microscopy, we visualized spots of Ca(2+) influx across individual MS channels distributed near focal adhesions in the basal surface of HUVECs. This study provides the first direct evidence that the cytoskeleton works as a force-transmitting and force-focusing molecular device to activate MS channels in eukaryotic cells.
Electroconvulsive Stimuli Alter the Regional Concentrations of Nerve Growth Factor, Brain-Derived Neurotrophic Factor, and Glial Cell Line-Derived Neurotrophic Factor in Adult Rat Brain (2002)

In this study we investigated whether electroconvulsive stimuli (ECS) altered the regional brain protein concentrations of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neurotrophic factor (GDNF) in Sprague Dawley rats. Electroconvulsive stimuli were administered once daily for 8 days. At the end of the experiment, rats were killed, the brains were dissected into five regions, and the neurotrophic factors were extracted and measured by enzyme-linked immunosorbent assay. Electroconvulsive stimuli increased the concentrations of NGF in the frontal cortex and concentrations of BDNF in the hippocampus, the striatum, and the occipital cortex. In contrast, ECS decreased GDNF concentrations in the hippocampus and the striatum. Our data indicate that neurotrophic factors play a role in the mechanism of action of ECS and, by extrapolation, may play a role in the mechanism of action of electroconvulsive treatment.
Time‐resolved NMR studies of RNA folding (2007)

The application of real-time NMR experiments to the study of RNA folding, as reviewed in this article, is relatively new. For many RNA folding events, current investigations suggest that the time scales are in the second to minute regime. In addition, the initial investigations suggest that different folding rates are observed for one structural transition may be due to the hierarchical folding units of RNA. Many of the experiments developed in the field of NMR of protein folding cannot directly be transferred to RNA: hydrogen exchange experiments outside the spectrometer cannot be applied since the intrinsic exchange rates are too fast in RNA, relaxation dispersion experiments on the other require faster structural transitions than those observed in RNA. On the other hand, information derived from time-resolved NMR experiments, namely the acquisition of native chemical shifts, can be readily interpreted in light of formation of a single long-range hydrogen bonding interaction. Together with mutational data that can readily be obtained for RNA and new ligation technologies that enhance site resolution even further, time-resolved NMR may become a powerful tool to decipher RNA folding. Such understanding will be of importance to understand the functions of coding and non-coding RNAs in cells.
Impact of data placement on resilience in large-scale object storage systems (2016)

Distributed object storage architectures have become the de facto standard for high-performance storage in big data, cloud, and HPC computing. Object storage deployments using commodity hardware to reduce costs often employ object replication as a method to achieve data resilience. Repairing object replicas after failure is a daunting task for systems with thousands of servers and billions of objects, however, and it is increasingly difficult to evaluate such scenarios at scale on real-world systems. Resilience and availability are both compromised if objects are not repaired in a timely manner. In this work we leverage a high-fidelity discrete-event simulation model to investigate replica reconstruction on large-scale object storage systems with thousands of servers, billions of objects, and petabytes of data. We evaluate the behavior of CRUSH, a well-known object placement algorithm, and identify configuration scenarios in which aggregate rebuild performance is constrained by object placement policies. After determining the root cause of this bottleneck, we then propose enhancements to CRUSH and the usage policies atop it to enable scalable replica reconstruction. We use these methods to demonstrate a simulated aggregate rebuild rate of 410 GiB/s (within 5% of projected ideal linear scaling) on a 1,024-node commodity storage system. We also uncover an unexpected phenomenon in rebuild performance based on the characteristics of the data stored on the system.
Few-mode fiber transmission with in-line few-mode erbium-doped fiber amplifier (2013)

We demonstrate wavelength-division multiplexed (WDM) and mode-division multiplexed (MDM) transmission over a fiber recirculating loop comprising of a 25-km span of low differential mode group dispersion (DMGD) few-mode fiber carrying the LP01 and LP11 mode groups, and an inline few-mode erbium-doped fiber amplifier (FM-EDFA) providing low mode-dependent gain (MDG) per span. We successfully transmitted a 10λ × 6 × 28-Gbaud QPSK signal over a distance of 700 km.
Sybase SQL Server on the World Wide Web (1996)

From the Publisher:
As the World Wide Web becomes the medium for interactive business communications and electronic commerce, companies need to know how to connect their Web pages to their enterprise database-management system. This book shows software developers and system administrators how to build interactive Web applications. From this comprehensive guide you'll learn how to analyze your company's needs and objectives, select the right approach for creating a Web-to-database link, choose the best tools to implement your strategy - even how to make sure your data is accessible and secure. Exhaustively illustrated with examples and sample code, Sybase SQL Server on the World Wide Web covers the leading languages and tools for Web development including: HTML Forms, CGI, Perl, Tcl, Java, ISQL, and Web.sql. Also includes an in-depth discussion on security encryption including symmetric (secret key), asymmetric (public key), message digests, and digital certificates methods.
Multi-resolution vehicle detection using artificial vision (2004)

This paper describes a vehicle detection system using a single camera. It is based on the search for areas with a high vertical symmetry in multi-resolution images; symmetry is computed using different sized boxes centered on all the columns of the interest areas. All the columns with high symmetry are analyzed to get the width of detected objects. Horizontal edges are examined to find the base of the vehicle in the individuated area. The aim is to find horizontal lines located below an area with sufficient amount of edges. The algorithm deletes all the bounding boxes which are too large, too small, or too far from the camera in order to decrease the number of false positives. All the results found in different interest areas are mixed together and the overlapping bounding boxes are localized and managed in order to delete false positives. The algorithm analyzes images on a frame by frame basis, without any temporal correlation.
MeshWorks: A Comprehensive Framework for Optimized Clock Mesh Network Synthesis (2010)

Clock mesh networks are well known for their variation tolerance. But their usage is limited to high-end designs due to the significantly high resource requirements compared to clock trees and the lack of automatic mesh synthesis tools. Most existing works on clock mesh networks either deal with semi-custom design or perform optimizations on a given clock mesh. However, the problem of obtaining a good initial clock mesh has not been addressed. Also, the problem of achieving a smooth tradeoff between variation tolerance and resource requirements has not been addressed adequately. In this paper, we present our MeshWorks framework, the first comprehensive automated framework for planning, synthesis, and optimization of clock mesh networks that addresses the above issues. Experimental results suggest that our algorithms can achieve an additional reduction of 31% in buffer area, 21% in wirelength, and 23% in power, compared to the best previous work, with similar worst case maximum frequency. We also demonstrate the effectiveness of our framework under several practical issues such as blockages, multiple clocks, uneven load distribution, and electromigration violations.
Characterization of a novel human putative mitochondrial transporter homologous to the yeast mitochondrial RNA splicing proteins 3 and 4 (2001)

We report here a novel human gene, hMRS3/4, encoding a putative mitochondrial transporter structurally and functionally homologous to the yeast mitochondrial RNA splicing proteins 3 and 4. These proteins belong to the family of mitochondrial carrier proteins (MCF) and are likely to function as solute carriers. hMRS3/4 spans approximately 10 kb of genomic DNA on chromosome 10q24 and consists of four exons that encode a 364-aa protein with six transmembrane domains. A putative splice variant, encoding a 177-aa protein with three transmembrane domains, was also identified. hMRS3/4 has a well-conserved signature sequence of MCF and is targeted into the mitochondria. When expressed in yeast, hMRS3/4 efficiently restores the mitochondrial functions in mrs3(o)mrs4(o) knock-out mutants. Ubiquitous expression in human tissues and a well-conserved structure and function suggest an important role for hMRS3/4 in human cells.
Randomized Controlled Trial of RSS Reader Use and Resident Familiarity With Primary Literature (2014)

The RSS reader use was accepted by residents and associated with increased familiarity with the primary literature but not with increased understanding.
An efficient fair payment system (1996)

Many proposed payment systems allow the payer to remain anonymous during a transaction. However, this unconditional privacy protection could be misused by criminals, e.g. for blackmailing or money laundering. With a fair payment system, anonymous payments are still possible, but the anonymity can be removed with the help of a trusted party which need not be involved in the transaction itself. In this paper, we present an efficient fair payment system and we discuss its security.
Adaptive Control of Systems with Actuator and Sensor Nonlinearities (1996)

From the Publisher:
An in-depth examination of intelligent approaches to increasing the accuracy of a variety of system components. Utilizing a unified, adaptive, inverse approach, the book offers electrical, mechanical, chemical, aeronautical and computer engineers methods for controlling many of the hard nonlinearities of frequently-employed control systems such as dead-zone, backlash and hysteresis. Discusses such nonlinearities at both the input and output points of a linear part and within both continuous time designs and discrete time designs.
Omega-3 fatty acid supplementation and cardiovascular disease (2012)

Epidemiological studies on Greenland Inuits in the 1970s and subsequent human studies have established an inverse relationship between the ingestion of omega-3 fatty acids [C(20-22) ω 3 polyunsaturated fatty acids (PUFA)], blood levels of C(20-22) ω 3 PUFA, and mortality associated with cardiovascular disease (CVD). C(20-22) ω 3 PUFA have pleiotropic effects on cell function and regulate multiple pathways controlling blood lipids, inflammatory factors, and cellular events in cardiomyocytes and vascular endothelial cells. The hypolipemic, anti-inflammatory, anti-arrhythmic properties of these fatty acids confer cardioprotection. Accordingly, national heart associations and government agencies have recommended increased consumption of fatty fish or ω 3 PUFA supplements to prevent CVD. In addition to fatty fish, sources of ω 3 PUFA are available from plants, algae, and yeast. A key question examined in this review is whether nonfish sources of ω 3 PUFA are as effective as fatty fish-derived C(20-22) ω 3 PUFA at managing risk factors linked to CVD. We focused on ω 3 PUFA metabolism and the capacity of ω 3 PUFA supplements to regulate key cellular events linked to CVD. The outcome of our analysis reveals that nonfish sources of ω 3 PUFA vary in their capacity to regulate blood levels of C(20-22) ω 3 PUFA and CVD risk factors.
The role of statins in cancer prevention and treatment. (2005)

Statins inhibit the activity of the rate-limiting enzyme in the cholesterol biosynthetic pathway, HMG-CoA reductase, and are widely prescribed for lowering plasma lipid levels. Several statins have antitumor effects in experimental models, and observational studies suggest that this anticancer activity in the laboratory may translate into effective treatments and/or preventive strategies for certain human cancers. This paper reviews the laboratory and clinical evidence that statins have anticancer activity, discusses the possible mechanisms by which tumor growth may be inhibited by this class of drugs, and outlines strategies for the evaluation of these agents in the prevention and treatment of human cancers.
REGULATION OF HER2/NEU GENE-EXPRESSION (REVIEW) (1995)

Transcriptional regulation of the HER2/neu protooncogene (also known as c-erbB2) has been the topic of many recent reports. The importance of these studies lies in the fact that HER2/neu overexpression occurs in about 30% of breast and ovarian cancers and to varying degrees in other cancers, including gastric, colorectal, lung, salivary, ovarian, bladder, pancreas, endometrial, cervical, oral, and prostate cancers. Furthermore, increased levels of the gene product play an important role during the neoplastic process of certain tumors. This review summarizes the recent work studying the regulation of HER2/neu expression in cells that overexpress the gene and reviews the current knowledge on general regulation of the HER2/neu promoter.
Miniemulsion Living Free Radical Polymerization of Styrene (2000)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTMiniemulsion Living Free Radical Polymerization of StyreneAlessandro Butté,, Giuseppe Storti, and Massimo MorbidelliView Author Information Laboratorium für Technische Chemie LTC, ETH Zürich, CAB C40, Universitätstr. 6, CH-8092 Zürich, Switzerland Cite this: Macromolecules 2000, 33, 9, 3485–3487Publication Date (Web):April 10, 2000Publication History Received4 November 1999Revised4 February 2000Published online10 April 2000Published inissue 1 May 2000https://pubs.acs.org/doi/10.1021/ma991866whttps://doi.org/10.1021/ma991866wbrief-reportACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views810Altmetric-Citations92LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Emulsions,Polymer particles,Radical polymerization,RAFT polymerization,Styrenes Get e-Alerts
Server+ Certification Bible with CD-ROM (2001)

From the Publisher:
The Server+ Certification Bible is a combination of information drawn right from the Server+ test objectives. It focuses on subject matter mastery, complete with review questions to prepare you for the exam. Topics focus on vendor-neutral knowledge covering multiple server types, server installation procedures, configuration and upgrading, advanced hardware issues, proactive maintenance, network cabling and connecting, troubleshooting, SCSI, multiple CPU's and RAID and will addressed a level of knowledge above basic hardware issues. As with all books in the Certification Bible series, The Server+ Certification Bible relies on logical organization, practice, and real-world examples to facilitate learning beyond the exam.
A transforming KIF5B and RET gene fusion in lung adenocarcinoma revealed from whole-genome and transcriptome sequencing (2011)

The identification of the molecular events that drive cancer transformation is essential to the development of targeted agents that improve the clinical outcome of lung cancer. Many studies have reported genomic driver mutations in non-small-cell lung cancers (NSCLCs) over the past decade; however, the molecular pathogenesis of >40% of NSCLCs is still unknown. To identify new molecular targets in NSCLCs, we performed the combined analysis of massively parallel whole-genome and transcriptome sequencing for cancer and paired normal tissue of a 33-yr-old lung adenocarcinoma patient, who is a never-smoker and has no familial cancer history. The cancer showed no known driver mutation in EGFR or KRAS and no EML4-ALK fusion. Here we report a novel fusion gene between KIF5B and the RET proto-oncogene caused by a pericentric inversion of 10p11.22-q11.21. This fusion gene overexpresses chimeric RET receptor tyrosine kinase, which could spontaneously induce cellular transformation. We identified the KIF5B-RET fusion in two more cases out of 20 primary lung adenocarcinomas in the replication study. Our data demonstrate that a subset of NSCLCs could be caused by a fusion of KIF5B and RET, and suggest the chimeric oncogene as a promising molecular target for the personalized diagnosis and treatment of lung cancer.