gebhart/patrepeval huggingface dataset:
Intrinsic Tyrosine Fluorescence as a Tool To Study the Interaction of the Shaker B “Ball” Peptide with Anionic Membranes (2003)

Steady-state and time-resolved fluorescence from the single tyrosine in the inactivating peptide of the Shaker B potassium channel (ShB peptide) and in a noninactivating peptide mutant, ShB-L7E, has been used to characterize their interaction with anionic phospholipid membranes, a model target mimicking features of the inactivation site on the channel protein. Partition coefficients derived from steady-state anisotropy indicate that both peptides show a high affinity for anionic vesicles, being higher in ShB than in ShB-L7E. Moreover, differential quenching by lipophilic spin-labeled probes and fluorescence energy transfer using trans-parinaric acid as the acceptor confirm that the ShB peptide inserts deep into the membrane, while the ShB-L7E peptide remains near the membrane surface. The rotational mobility of tyrosine in membrane-embedded ShB, examined from the decay of fluorescence anisotropy, can be described by two different rotational correlation times and a residual constant value. The short correlation time corresponds to fast rotation reporting on local tyrosine mobility. The long rotational correlation time and the high residual anisotropy suggest that the ShB peptide diffuses in a viscous and anisotropic medium compatible with the aliphatic region of a lipid bilayer and support the hypothesis that the peptide inserts into it as a monomer, to configure an intramolecular beta-hairpin structure. Assuming that this hairpin structure behaves like a rigid body, we have estimated its dimensions and rotational dynamics, and a model for the peptide inserted into the bilayer has been proposed.
Progressive ventricular remodeling in rat with myocardial infarction (1991)

Ventricular dilatation may have important prognostic implications for the survival of patients with left ventricular (LV) dysfunction. To determine the manner and extent to which the left ventricle of the rat remodels and dilates after myocardial infarction, we obtained the passive pressure-volume relationships, chamber stiffness constants, and mass during both the early and late phases. In moderate and large infarcts as inflammation and edema developed, LV weight increased then progressively decreased as a thin scar formed, returning to normal values as a result of compensatory hypertrophy of the residual myocardium. LV dilatation occurred in all rats with infarcts but to different extents depending on infarct size and duration. In the early postinfarction phase, pressure-volume relationship was relatively unchanged in all infarct-size groups, except for significant rightward shift in low pressure range for rats with moderate and large infarcts and significant leftward shift in high pressure range for rats with small infarcts. During resolution of the inflammatory response, LV dilatation occurred in all infarct groups in relation to infarct size. As scar formation became complete, LV enlargement did not progress in rats with small infarcts but did so in rats with moderate and large infarcts. LV chamber stiffness remained within the range of normal values during the early phase in all rats with infarcts but decreased significantly during the late phase in rats with moderate and large infarcts in association with the extent of ventricular enlargement. Alterations in the volume-to-mass ratio (V/Vwt) were most marked in the late postinfarction phase, wherein both volume (increased) and mass (decreased, then increased) changed dramatically and V/Vwt progressively increased in rats with large infarcts.
Using program slicing in software maintenance (1991)

Program slicing is applied to the software maintenance problem by extending the notion of a program slice (that originally required both a variable and line number) to a decomposition slice, one that captures all computation on a given variable, i.e., is independent of line numbers. Using the lattice of single variable decomposition slices ordered by set inclusion, it is shown how a slice-based decomposition for programs can be formed. One can then delineate the effects of a proposed change by isolating those effects in a single component of the decomposition. This gives maintainers a straightforward technique for determining those statements and variables which may be modified in a component and those which may not. Using the decomposition, a set of principles to prohibit changes which will interfere with unmodified components is provided. These semantically consistent changes can then be merged back into the original program in linear time.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
In situ infrared external reflection spectroscopy as a probe of the interactions at the liquid-solid interface of long-chain alkanethiol monolayers at gold (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn situ infrared external reflection spectroscopy as a probe of the interactions at the liquid-solid interface of long-chain alkanethiol monolayers at goldScott M. Stole and Marc D. PorterCite this: Langmuir 1990, 6, 6, 1199–1202Publication Date (Print):June 1, 1990Publication History Published online1 May 2002Published inissue 1 June 1990https://pubs.acs.org/doi/10.1021/la00096a029https://doi.org/10.1021/la00096a029research-articleACS PublicationsRequest reuse permissionsArticle Views187Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A highly conserved candidate chemoreceptor expressed in both olfactory and gustatory tissues in the malaria vector Anopheles gambiae (2004)

Anopheles gambiae is a highly anthropophilic mosquito responsible for the majority of malaria transmission in Africa. The biting and host preference behavior of this disease vector is largely influenced by its sense of smell, which is presumably facilitated by G protein-coupled receptor signaling [Takken, W. & Knols, B. (1999) Annu. Rev. Entomol. 44, 131-157]. Because of the importance of host preference to the mosquitoes' ability to transmit disease, we have initiated studies intended to elucidate the molecular mechanisms underlying olfaction in An. gambiae. In the course of these studies, we have identified a number of genes potentially involved in signal transduction, including a family of candidate odorant receptors. One of these receptors, encoded by GPRor7 (hereafter referred to as AgOr7), is remarkably similar to an odorant receptor that is expressed broadly in olfactory tissues and has been identified in Drosophila melanogaster and other insects [Krieger, J., Klink, O., Mohl, C., Raming, K. & Breer, H. (2003) J. Comp. Physiol. A 189, 519-526; Vosshall, L. B., Amrein, H., Morozov, P. S., Rzhetsky, A. & Axel, R. (1999) Cell 96, 725-736]. We have observed AgOr7 expression in olfactory and gustatory tissues in adult An. gambiae and during several stages of the mosquitoes' development. Within the female adult peripheral chemosensory system, antiserum against the AgOR7 polypeptide labels most sensilla of the antenna and maxillary palp as well as a subset of proboscis sensilla. Furthermore, AgOR7 antiserum labeling is observed within the larval antenna and maxillary palpus. These results are consistent with a role for AgOr7 in both olfaction and gustation in An. gambiae and raise the possibility that AgOr7 orthologs may also be of general importance to both modalities of chemosensation in other insects.
Low-frequency noise dependence of TFSOI BiCMOS for low power RF mixed-mode applications (2002)

Low-frequency (1/f) noise has been characterized for the first time in TFSOI BiCMOS devices designed for low power high frequency applications. In the bipolar transistors, 1/f noise obeyed a square law dependence on base current and was proportional to the inverse of the area. Aside from the expected 1/f noise, we have also observed a bias dependent generation-recombination (G/R) noise component in a small portion of these TFSOI BJTs. The 1/f noise in the near-fully-depleted MOSFETs was found to be bias independent in both the linear and saturation region of operation. However, when operated in the subthreshold regime, extraneous generation-recombination (G/R) noise becomes apparent.
A Theoretical Study of the Three Phase Salient Pole Type Generator with Simultaneous AC and Bridge Rectified DC Output-Part I (1973)

This paper presents theoretical equations, describing the relations between the performance and design details of a three phase salient pole type generator with simultaneous AC and DC output, the latter being obtained by bridge rectification. The AC voltage is regulated and the DC voltage floats; however, the inverse condition is also included. The start and duration of DC commutation, all damper and field winding currents, the DC commutation current and both average, RMS and instantaneous DC and AC voltages are determined.
Osteomalacia with low alkaline phosphatase: a not so rare condition with important consequences (2016)

Hypophosphatasia is a genetic disorder, characterised by a dysfunctional tissue-non-specific isoenzyme of alkaline phosphatase that impacts bone metabolism and predisposes to osteomalacia or rickets. The clinical presentation is very diverse, depending on the age of onset and the severity of the disease. Several forms of hypophosphatasia are recognised. We present a case of a 50-year-old woman with low impact fractures and loss of teeth at a young age. She also had a low alkaline phosphatase and was diagnosed with adult hypophosphatasia. Although the severe forms of hypophosphatasia are rather rare, the adult form is thought to occur quite frequently. As this condition is not well known by healthcare professionals, the time to diagnosis and initiation of adequate treatment is often postponed. When encountering a patient with low alkaline phosphatase, low bone density or a history of bone fractures, the possibility of hypophosphatasia should be considered.
The Necessary Nitrogen Atom: A Versatile High-Impact Design Element for Multiparameter Optimization (2017)

There is a continued desire in biomedical research to reduce the number and duration of design cycles required to optimize lead compounds into high-quality chemical probes or safe and efficacious drug candidates. The insightful application of impactful molecular design elements is one approach toward achieving this goal. The replacement of a CH group with a N atom in aromatic and heteroaromatic ring systems can have many important effects on molecular and physicochemical properties and intra- and intermolecular interactions that can translate to improved pharmacological profiles. In this Perspective, the "necessary nitrogen atom" is shown to be a versatile high-impact design element for multiparameter optimization, wherein ≥10-, 100-, or 1000-fold improvement in a variety of key pharmacological parameters can be realized.
Transfer of Genetic Information by Purified Metaphase Chromosomes (1973)

Transfer of genetic information from isolated mammalian chromosomes to recipient cells has been demonstrated. Metaphase chromosomes isolated from Chinese hamster fibroblasts were incubated with mouse A(9) cells containing a mutation at the hypoxanthine-guanine phosphoribosyl transferase (hprt) locus. Cells were plated in a selective medium, resulting in death of all unaltered parental A(9) cells. However, colonies of cells containing hypoxanthine phosphoribosyl transferase (EC 2.4.2.8) appeared with a variable frequency of about 10(-6) to 10(-7). The enzyme from these cells was indistinguishable from that from Chinese hamster cells, as shown by DEAE-cellulose chromatography and gel electrophoresis, and differed clearly from the mouse enzyme. The colonies, thus, did not result from reversion of A(9) parental cells to wild type, but appeared to represent progeny of individual cells that had ingested chromosomes, replicated, and expressed the hprt gene. These colonies differed from each other in stability of expression of the transferred gene.
Selective Aerobic Oxidation of Alcohols with a Combination of a Polyoxometalate and Nitroxyl Radical as Catalysts (2001)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTSelective Aerobic Oxidation of Alcohols with a Combination of a Polyoxometalate and Nitroxyl Radical as CatalystsRevital Ben-Daniel, Paul Alsters, and Ronny NeumannView Author Information Department of Organic Chemistry, Weizmann Institute of Science, Israel, 76100, and DSM Research, Koestraat 1, 6160 MD Geleen, The Netherlands [email protected]Cite this: J. Org. Chem. 2001, 66, 25, 8650–8653Publication Date (Web):November 16, 2001Publication History Received11 June 2001Published online16 November 2001Published inissue 1 December 2001https://pubs.acs.org/doi/10.1021/jo0105843https://doi.org/10.1021/jo0105843brief-reportACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views1820Altmetric-Citations129LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alcohols,Anions,Oxidation,Pharmaceuticals,Photovoltaics Get e-Alerts
A study on the realtionships between stochastic and harmonic coding (2005)

Two of the coding methods which have appeared recently, attempting to solve the problem of high quality speech coding at bit rates below 9.6 kbits/sec are harmonic and stochastic coding. The two represent distinct approaches to this problem and yield synthetic speech of a very different kind. In this paper, we discuss the advantages and disadvantages of both methods for processing voiced and unvoiced speech segments, and examine the potential benefits of combining the two methods in coding applications.
Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis (1996)

The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism (SSCP) analysis. The central region of the ospA gene of B. burgdorferi from infected ticks was amplified by nested PCR. Amplified product of the correct size was obtained from 20 to 45 ticks (44%). This is the fraction of ticks that is expected to be infected with B. burgdorferi. Four variant classes were determined by SSCP analysis. Eight ticks were infected with a single variant, nine ticks were infected with two variants, two ticks were infected with three variants, and one tick was infected with all four variants. DNA from each variant was sequenced. Five different sequences were found. The sequence of each variant was different from that of another variant by a single base. SSCP analysis could distinguish three of the four single-base changes found in the region.
Bandwidth-efficient trellis coded modulation schemes (1990)

The author proposes a scheme that shapes the power density spectrum of the transmitted signal to reduce its bandwidth. The encoder is modeled as a finite-state machine consisting of a shift register and a mapper. A pulse-shaping filter generates time-limited or Nyquist pulses. The whole system is optimized with respect to bandwidth and power efficiency, where average-power and peak-power criteria are considered. The author presents the achievable data rates of systems with quaternary input symbols and an out-of-band power of -20 dB for the various mappers and pulse-shaping filters considered. It can be seen that bandlimited pulses allow roughly a doubling of the data rate with respect to time-limited pulses. The bandwidth gain that can be reached with proper coding is remarkable. The drawback is the increased receiver complexity needed to resolve the intersymbol interference.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Regulated Dimerization of the Thyrotropin-Releasing Hormone Receptor Affects Receptor Trafficking But Not Signaling (2005)

To investigate the function of dimerization of the TRH receptor, a controlled dimerization system was developed. A variant FK506 binding protein (FKBP) domain was fused to the receptor C terminus and dimerization induced by incubating cells with dimeric FKBP ligand, AP20187. The TRH receptor-fusion bound hormone and signaled normally. Addition of dimerizer to cells expressing the receptor-FKBP fusion dramatically increased the fraction of receptor running as dimer on SDS-PAGE. AP20187 caused dimerization in a time- and concentration-dependent manner, acting within 1 min. Dimerizer had no effect on TRH receptors lacking the FKBP domain, and its effects were blocked by excess monomeric FKBP ligand. AP20187-induced dimerization did not cause receptor phosphorylation, inositol phosphate production, or ERK1/2 activation, and dimerizer did not alter signaling by TRH. Induced dimerization did, however, alter TRH receptor trafficking. TRH promoted greater receptor internalization in cells treated with AP20187 but not monomeric ligand, based on loss of surface binding sites and immunostaining. Dimerization increased the rate of internalization of TRH receptors and decreased the apparent rate of receptor recycling. AP20187 enhanced the small amount of TRH-induced receptor internalization when the receptor-FKBP fusion protein was expressed in cells lacking beta-arrestins. The results show that controlled dimerization of the TRH receptor potentiates hormone-induced receptor trafficking.
RPC: Remote Procedure Call Protocol Specification Version 2 (1995)

This document describes the ONC Remote Procedure Call (ONC RPC Version
2) protocol as it is currently deployed and accepted. [STANDARDS-
TRACK]
Directions in TeraHertz technology (2002)

The term 'T-rays' was coined in the early 1990's by Bell Labs to describe the spectrum in the TeraHertz range (1 THz=10/sup 12/ Hz). The THz region of the spectrum lies on the border of where electronics and optics meet, between the mm-wave and infrared bands. Infrared sources become very dim as we approach the THz region and high speed devices struggle to reach these frequencies. Consequently, practical 0.1 THz to 20 THz sources were difficult to obtain until recent developments in laser, quantum well and compound semiconductor technology in the 1990's. Historically, we know that each time a new band in the electromagnetic spectrum opens up, a whole new industry develops and revolves around that band.
Corrosion Behavior of Coarse- and Fine-Grain Ni Coatings Incorporating NaH2PO4.H2O Inhibitor Treated Substrates (2015)

Plain carbon steel substrates were treated with NaH2PO4.H2O inhibitor for 24 hours and coated with Ni using dc and pulse electrodeposition in standard Watt’s bath. The effect of dc and pulse electrodeposition, on the microstructure and corrosion properties of Ni coatings in 3.5 wt% NaCl solution was studied. The effect of inhibitor on the deposition process and corrosion behavior was also examined. Materials characterization was performed using field emission scanning electron microscopy, cross-sectional scanning transmission electron microscopy, atomic force microscopy, x-ray diffraction and nanoindentation. Experimental results indicated that pulse electrodeposition produced fine grained Ni coatings that showed lower corrosion rate compared to coarse grained dc electrodeposited Ni. Pre-treatment of substrates with inhibitor did not adversely affect the deposition process and adherent Ni coatings were readily developed. The results showed that pulse electrodeposition could be used to produce hard corrosion resistant Ni coatings while the inhibitor treatment yielded enhanced corrosion protection by providing a protective buffer layer between the Ni coating and the substrate.
Challenges with real-world smartwatch based audio monitoring (2018)

Audio data from a microphone can be a rich source of information. The speech and audio processing community has explored using audio data to detect emotion, depression, Alzheimer's disease and even children's age, weight and height. The mobile community has looked at using smartphone based audio to detect coughing and other respiratory sounds and help predict students' GPA. However, audio data from these studies tends to be collected in more controlled environments using well placed, high quality microphones or from phone calls. Applying these kinds of analyses to continuous and in-the-wild audio could have tremendous applications, particularly in the context of health monitoring. As part of a health monitoring study, we use smartwatches to collect in-the-wild audio from real patients. In this paper we characterize the quality of the audio data we collected. Our findings include that the smartwatch based audio is good enough to discern speech and respiratory sounds. However, extracting these sounds is difficult because of the wide variety of noise in the signal and current tools perform poorly at dealing with this noise. We also find that the quality of the microphone allows annotators to differentiate the source of speech and coughing, which adds another level of complexity to analyzing this audio.