gebhart/patrepeval huggingface dataset:
Novel ubiquitin-like ribosomal protein fusion genes from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae. (1993)

Among eukaryotes studied to date, homologs of the yeast 76-amino acid ribosomal protein have invariably been found to be cotranslated with ubiquitin. However, in the nematodes Caenorhabditis elegans and Caenorhabditis briggsae, a 70-amino acid domain with only 40% identity to ubiquitin is cotranslated with a homolog of the ribosomal protein. In the nematode ubiquitin-like (UbL) proteins, the nucleotide sequence of the UbL coding region is 92% identical in C. elegans and C. briggsae. The corresponding gene sequence contains a single intron at a location identical to that found in the polyubiquitin gene of C. elegans, further confirming that the ubl genes are evolutionarily related to ubiquitin. The ribosomal protein portion of the UbL polypeptide consists of 93 amino acids and is 68% identical to the human homolog. The ribosomal protein portion of UbL is longer than in other homologs, with the additional sequence being present as a basic carboxyl extension. The ubl gene is constitutively expressed in all life cycle stages of C. elegans. A comparison of the nematode UbL sequences with other ubiquitin-like genes reveals a pattern of sequence conservation, which suggests that the ubiquitin-like proteins may have conserved functional domains.
cDNA cloning and expression of the human A-type platelet-derived growth factor (PDGF) receptor establishes structural similarity to the B-type PDGF receptor. (1989)

The primary structure of the human A-type receptor for platelet-derived growth factor (PDGF) has been determined. A 6.5-kilobase (kb) transcript was identified through low-stringency hybridization with a probe derived from the B-type PDGF receptor cDNA. The sequence of a cDNA clone corresponding to the 6.5-kb transcript contains an open reading frame that predicts a 1089-amino acid growth factor receptor-like molecule, which displays 44% overall amino acid similarity with the PDGF B-type receptor. The two receptors have a similar domain organization, with five immunoglobulin-like domains extracellularly and an intracellular split protein tyrosine kinase domain. Transfection of the new cDNA into COS cells led to the expression of a protein specifically recognized by an antiserum previously shown to react with the PDGF A-type receptor. The expressed protein was shown to display high-affinity binding of all three 125I-labeled dimeric forms of PDGF A and B chains in a manner that is characteristic for the PDGF A-type receptor.
Light induced electron transfer reactions of metal complexes (1980)

Properties of the excited states of tris(2,2'-bipyridine) and tris(l,lO-phenanthroline) complexes of chromium(III), iron(II), ruthenium(II), osmium(II), rhodium(III), and iridium(III) are described. The electron transfer reactions of the ground and excited states are discussed and interpreted in terms of the driving force for the reaction and the distortions of the excited states relative to the corresponding ground states. General considerations relevant to the conversion of light into chemical energy are presented and progress in the use of polypyridine complexes to effect the light induced decomposition of water into hydrogen and oxygen is reviewed. ''l.O <i02 0.0008 4.0 'O.9 Ru(bpy)32+ 4d6 MLCT 1.0 103 0.6 .O.2 2.1 Os(bpy)32+ 5d6 MLCT ---0.019 O.l 1.8 Rh(phen)33 4d6 L(r_7r*) l.O --0.3 <0.5 2.8 Ir(bpy)33 5d6 L(,r_,r*) --2.4 'O.1 2.8 CuL2+ 3d1M LCT(?) -------<2.5 a Unless otherwise stated, the data are for aqueous solutions at room temperature and were assembled from ref 1-3. bpy = 2,2'-bipyridine, L = 2, 9-dimethyl-1 , lO-phenanthroline. b antum yield for excited state formation: data for Cr(bpy)33+ from ref 5, for Fe(bpy)32+ from ref 7, for Ru(bpy)32+ from ref 12, for Rh(phen)33+ from ref 11. c Photoaquation quantum yield: for Cr(bpy)33+ at pH < 4.5 and > 9.0, respectively, from ref 5; for Fe(bpy)32+ from ref 7; for Ru(bpy)32+ in 1 M HC1 from ref 13. d Lifetime in H20 (ref 1-3) except for Rh(phen)33+ (acetonitrile, ref 11) and Ir(bpy)33 (methanol, ref 10). e Stokes shifts from refs 14, 15, 26, 17, and 9 for Cr, Ru, Os, Rh, and Ir, respectively. The value given for Fe is not a Stokes shift, as the complex does not emit, but is twice the estimated excited state distortion (7). f Excited state energy; that for Fe(bpy)32+ was estimated in ref 7. E* for CuL2+ is bracketed by the energy of the band at 450 nm.
Serial Analysis of Gene Expression in Non-Small Cell Lung Cancer1 (1998)

We used the serial analysis of gene expression (SAGE) method to systematically analyze transcripts present in non-small cell lung cancer. Over 226,000 SAGE tags were sequence analyzed from two independent primary lung cancers and two normal human bronchial/trachea) epithe lial cell cultures. A total of 226,000 SAGE tags were sequence identified. representing 43,254 unique transcripts. Comparison of the tags present in the tumor with those identified in the normal tissue revealed 175 tran script tags that were overrepresented in the normal tissue and 142 tags that were overexpressed in the tumor by 10-fold or more. Northern hybridization was performed on 15 of the most abundantly expressed tags identified in the tumors. These tags were derived from either a known gene or a matched expressed sequence tag clone. The transcripts for 3 of the 15 genes, PGP 9.5, li-myh. and human inui'f, were abundantly ex pressed in primary lung cancers (10 of 18, 15 of 18, and 6 of 12 tumors, respectively). In contrast, the presence of PGP9.5 and B-myb was much less frequent in primary tumors derived from other tissue origins. These results suggest that at least a portion of the transcripts identified by SAGE are frequently associated with lung cancer, and that their overexpression may contribute to lung tumorigenesis. The identification and further characterization of genes generated by SAGE should provide potential new targets for the diagnosis, prognosis, and therapy of lung cancer.
Antialiasing inputs for charge coupled devices (1976)

An input scheme for analog charge-coupled signal-processing devices has been developed that acts as a prefilter to suppress the aliasing frequencies produced by the sampled-data nature of the CCD. Each charge packet injected into the CCD is a sum of several weighted subsamples taken at a multiple of the CCD clock rate. Various approaches that differ in their rates of oversampling and in their time periods over which subsamples are combined have been compared with respect to their usefulness to produce a sharp cutoff prefilter. A particular implementation using only a factor of 2 oversampling but an averaging period that extends over two CCD clock periods results in a good prefilter characteristic and has been applied to a charge-coupled split-electrode transversal filter. Different implementations of the basic scheme and layout considerations are discussed.
SAR and temperature: Simulations and comparison to regulatory limits for MRI (2007)

Calculations indicate that both SAR and temperature distributions vary greatly with B(1) field frequency, that temperature distributions do not always correlate well with SAR distributions, and that regulatory limits on local temperature may not be exceeded as readily as those on local SAR.
Selective Expression of a Novel Surface Molecule by Human Th2 Cells In Vivo (1999)

The search for reliable marker molecules discriminating between human Th1 and Th2 cells identified a gene encoding a novel member of the G protein-coupled leukocyte chemoattractant receptor family, which is selectively expressed in Th2 but not Th1 lineage cells, thereby named CRTH2 (chemoattractant receptor-homologous molecule expressed on Th2 cells). Studies with anti-CRTH2 mAbs demonstrated that CRTH2 was expressed in a small population (0.4-6.5%) of CD4+ T cells in fresh PBMCs of healthy adults, but no remarkable expression was seen in B cells and NK cells. In some cases, CD8+ T cells ( approximately 3.5%) expressed CRTH2. Phenotypes of CD4+ T cells expressing CRTH2 were CD45RA-, CD45RO+, and CD25+, similar to those of Ag-activated effector/memory T cells. Freshly isolated CRTH2+ CD4+ T cells produced Th2- but little or no Th1-type cytokines upon stimulation with PMA and ionomycin. In addition, an allergen-induced proliferative response in fresh PBMCs was significantly and selectively reduced by subtracting CRTH2+ cells. Together, these results indicate that CRTH2 is selectively expressed in vivo in an activated state of Th2 cells including allergen-responsive Th2 cells, suggesting its pivotal roles in ongoing Th2-type immune reactions.
MHC Class-II Molecules and Autoimmunity (1991)

Molecular and genetic studies of HLA class-II genes provide new insights into the basis for MHC associations with autoimmunity. Polymorphisms among class-II genes identify specific haplotypes associated with autoimmune diseases such as type-I diabetes, rheumatoid arthritis, celiac disease, and pemphigus vulgaris. In some cases, single genes within those haplotypes are themselves implicated in disease susceptibility. Interactions, both cis and trans, between candidate susceptibility genes suggest a number of possible mechanisms critical for autoimmune triggering events involving class-II molecules. Amino acid sequence comparisons between products of candidate susceptibility genes and other class-II genes pinpoint a limited number of critical sites within HLA molecules which appear to be responsible for pathogenic events.
Native and baculovirus‐expressed forms of the immunoprotective protein BM86 from Boophilus microplus are anchored to the cell membrane by a glycosylphosphatidyl inositol linkage (1993)

A glycoprotein (BM86) from the gut cells of the cattle tick Boophilus microplus, when used to vaccinate cattle, has been shown to protect cattle from tick infestation. A recombinant BM86 protein is the principal component of a novel tick vaccine currently under development. The nature of the anchorage of BM86 to tick gut epithelial cells has been investigated using BM86 from B. microplus and recombinant BM86 proteins expressed in insect cells using the baculovirus expression system. BM86 from B. microplus and a full length recombinant BM86 are shown to be anchored to the extracellular surface of tick gut epithelial cells and baculovirus-infected insect cells, respectively by a glycosyl-phosphatidyl inositol membrane anchor. A recombinant BM86 truncated by the removal of a hydrophobic region coding for thirty amino acids at the carboxy-terminal end was secreted from baculovirus-infected Sf9 cells. This secreted form of recombinant BM86 showed strong protective activity against ticks in cattle vaccinated with this protein.
Current Management of Alcoholic Hepatitis and Future Therapies (2016)

Alcohol is one of the most common etiologies of liver disease, and alcoholic liver disease overall is the second most common indication for liver transplantation in the United States. It encompasses a spectrum of disease, including fatty liver disease, alcoholic hepatitis (AH), and alcoholic cirrhosis. AH can range from mild to severe disease, with severe disease being defined as: Discriminant Function (DF) ≥ 32, or Model for End-stage Liver Disease (MELD) ≥ 21, or presence of hepatic encephalopathy. Management of the mild disease consists mainly of abstinence and supportive care. Severe AH is associated with significant mortality. Currently, there is no ideal medical treatment for this condition. Besides alcohol cessation, corticosteroids have been used with conflicting results and are associated with an inherent risk of infection. Overall steroids have shown short term benefit when compared to placebo, but they have no obvious long term benefits. Pentoxifylline does not improve survival in patients with severe AH and is no longer recommended based on the results of the STOPAH (Steroid Or Pentoxifylline for Alcoholic Hepatitis) trial. Anti-tumor necrosis factor (TNF) agents are associated with increased risk of life threatening infections and death. Currently, early stage trials are underway, mainly targeting novel pathways based on disease pathogenesis, including modulation of innate immune system, inhibition of gut-liver axis and cell death pathways, and activation of transcription factor farnesyl X receptor (FXR). Future treatment may lie in human induced pluripotent stem cell (iPSC) technology, which is currently under investigation for the study of pathogenesis, drug discovery, and stem cell transplantation. Liver transplantation has been reported with good results in highly selected patients but is controversial due to limited organ supply.
Role of Methoxyestradiols in the Growth Inhibitory Effects of Estradiol on Human Glomerular Mesangial Cells (2002)

Metabolism of locally applied 17beta-estradiol (estradiol) to methoxyestradiols contributes to the growth inhibiting effects of estradiol on vascular smooth muscle cells via an estrogen receptor (ER)-independent mechanism. Because vascular smooth muscle cells are phenotypically similar to glomerular mesangial cells, it is feasible that estradiol inhibits glomerular mesangial cell growth via a similar mechanism, and this possibility was investigated. In human glomerular mesangail cells, estradiol concentration dependently (1 to 100 nmol/L) inhibited serum-induced proliferation (cell number) and DNA ((3)[H]-thymidine incorporation) and collagen ((3)[H]-proline incorporation) synthesis. The inhibitory effects of estradiol were mimicked by 2-hydroxyestradiol and 2-methoxyestradiol, metabolites of estradiol with little affinity for ERs. 2-Hydroxyestradiol and 2-methoxyestradiol were more potent growth inhibitors than estradiol. The inhibitory effects of estradiol were enhanced by CYP450 inducers 3-methylcholanthrene (10 micromol/L) and phenobarbital (10 micromol/L) and blocked by the CYP450 inhibitor 1-aminobenzotriazole (10 micromol/L). The growth inhibitory effects of estradiol were also blocked by quercetin (10 micromol/L) and OR 486 (10 micromol/L) inhibitors of catechol-O-methyltransferase (converts catecholestradiols to methoxyestradiols). ICI182780 (ER antagonist with ER binding affinity similar to estradiol) blocked the growth inhibitory effects of estradiol (1 to 100 nmol/L) only at concentrations (>50 micromol/L) that inhibited estradiol metabolism to catecholestradiols. The growth inhibitory effects of 2-hydroxyestradiol were abrogated by quercetin and OR486 (two structurally dissimilar catechol-O-methyltransferase inhibitors), but not by ICI182780. However, the growth inhibitory effects of 2-methoxyestradiol were unaltered by catechol-O-methyltransferase inhibitors and ICI182780. In conclusion, our findings provide the first evidence that methoxyestradiols mediate the growth inhibitory effects of locally applied estradiol on glomerular mesangial cell growth via an ER-independent mechanism.
Allogeneic bone marrow transplantation for leukemia: factors of importance for long-term survival and relapse. (1988)

From 1977 until June 1987, 139 patients with leukemia underwent allogeneic bone marrow transplantation at Huddinge Hospital. Among recipients of HLA identical bone marrow the survival plateau for 30 patients with acute myeloid leukemia in first remission was 71% from 3 to 7 years. Children (n = 11) had a survival of 100% compared with 52% (n = 19) in adults (p = 0.01). Among patients with acute lymphoblastic leukemia in first remission (n = 15) the actuarial 5-year survival was 73% compared with 31% for those (n = 25) in second to fourth remissions. The probability of relapse in these two groups was 9% and 45% respectively (p less than 0.05). Patients with chronic myeloid leukemia in first chronic phase (n = 27) had a survival plateau from 1 to 6 years of 65%. In Cox's multivariate regression analysis, improved survival was associated with grade 0-I acute graft-versus-host disease (GVHD) (p less than 0.0001), HLA-matched siblings (p less than 0.001), recipient age less than 17 years (p = 0.02), first remission and first chronic phase (p = 0.03), cytomegalovirus (CMV) seronegative recipients (p = 0.04) and absence of symptomatic CMV infection (p = 0.04). A decreased risk of relapse was seen in patients with first remission or first chronic phase (p less than 0.001) and in patients with asymptomatic CMV infection (p = 0.03). In multivariate analysis the p values were 0.002 and 0.09 for these two groups respectively. In these patients acute GVHD was the major obstacle to successful outcome.
Transmission of light through slit apertures in metallic films (2004)

Transmission of polarized light through sub-wavelength slit apertures is studied based on the electromagnetic field distributions obtained in computer simulations. The results show the existence of a cutoff for E|| and a strong transmission (with no cutoff) for E?; here || and ? refer to the direction of the incident E-field relative to the long axis of the slit. These observations are explained by the standard waveguide theory involving inhomogeneous plane waves that bounce back and forth between the interior walls of the slit aperture. We examine the roles played by the slit-width, by the film thickness, and by the real and imaginary parts of the host material's dielectric constant in determining the transmission efficiency. We also show that the slit's sharp edges can be rounded to eliminate highly-localized electric dipoles without significantly affecting the slit's throughput. Finally, interference among the surface charges and currents induced in the vicinity of two adjacent slits is shown to result in enhanced transmission through both slits when the slits are separated by about one half of one wavelength.
High mobilization of CD133+/CD34+ cells expressing HIF-1α and SDF-1α in septic abdominal surgical patients (2020)

ClinicalTrials.gov no. NCT02589535 . Registered 28 October 2015.
Optically Transparent Sulfur-Containing Polyimide−TiO2 Nanocomposite Films with High Refractive Index and Negative Pattern Formation from Poly(amic acid)−TiO2 Nanocomposite Film (2007)

A series of semialicyclic polyimides (PIs) have been successfully prepared by the polycondensation of two alicyclic dianhydrides, 1,2,3,4-cyclobutanetetracarboxylic dianhydride (CBDA) and 1,2,4,5-cyclohexanetetracarboxylic dianhydride (CHDA) with two sulfur-containing aromatic diamines, 4,4′-thiobis[(p-phenylenesulfanyl)aniline] (3SDA) and 2,7-bis(4-aminophenylenesulfanyl)thianthrene (APTT), respectively, by a two-step polymerization procedure via the soluble poly(amic acid) (PAA) precursors. Flexible and tough PI films were obtained with a tensile strength greater than 82 MPa and elongation at breaks higher than 12%. The films showed good thermal and thermal-oxidative stability without significant weight loss up to 400 °C, both in air and nitrogen. The glass-transition temperatures of the PIs ranged from 236.5 to 274.1 °C. Good optical transparency, including the cutoff wavelengths lower than 350 nm and transmittances higher than 80% at 400 nm, was achieved for all the PI films. The refractive indices of the PI films were in the range of 1.6799–1.7130 measured at 632.8 nm, which agreed well with the values calculated by the time-dependent density functional theory (TD-DFT). In addition, PAA-IIa (CHDA-3SDA) showed a good affinity with silica-modified TiO2 nanoparticles. The hybrid system with 45 wt % load of TiO2 nanoparticles, combined with the photobase generator N-{[(4,5-dimethoxy-2-nitrobenzyl)oxy]carbonyl}2,6-dimethylpiperidine (DNCDP) exhibited good photolithographic characteristics. A fine negative pattern with a resolution of approximately 4 µm was successfully printed on the hybrid film. The cured PI-IIa−TiO2 hybrid film exhibited a refractive index of 1.8100 at 632.8 nm.
Understanding early serum hepatitis D virus and hepatitis B surface antigen kinetics during pegylated interferon‐alpha therapy via mathematical modeling (2014)

Modeling results provide insights into HDV-host dynamics, the relationship between serum HBsAg levels and HBsAg-infected cells, IFN's mode of action, and its effectiveness. The observation that a flat second phase in HDV and HBsAg kinetics was associated with failure to achieve CVR provides the basis to develop early stopping rules during peg-IFN treatment in HDV-infected patients.
A direct preparation of vinylogous acyl anion equivalents (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA direct preparation of vinylogous acyl anion equivalentsC. Janakiram Rao and Paul KnochelCite this: J. Org. Chem. 1991, 56, 15, 4593–4596Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/jo00015a004https://doi.org/10.1021/jo00015a004research-articleACS PublicationsRequest reuse permissionsArticle Views605Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A Prospective Study of Aspirin Use and Primary Prevention of Cardiovascular Disease in Women (1991)

The use of one through six aspirin per week appears to be associated with a reduced risk of a first myocardial infarction among women. A randomized trial in women is necessary, however, to provide conclusive data on the role of aspirin in the primary prevention of cardiovascular disease in women.
Posttranslational Processing of Transfected Mouse Pro-Adrenocorticotropin/Endorphin in Rat Growth Hormone-Secreting Tumor Cells* (1989)

Pro-ACTH/endorphin (PAE; also POMC) is a hormone precursor that contains potential sites for endoproteolytic cleavage, glycosylation, phosphorylation, acetylation, sulfation, and amidation. Different sets of these sites are used in different neuroendocrine tissues. To learn more about the factors influencing the posttranslational processing of peptide hormone precursors, the cDNA for mouse PAE was cloned into a metallothionein expression vector. Two lines of rat GH-secreting tumor cells (GH3 and GC) were transfected with the resulting expression plasmid, and stable PAE-producing clones were isolated. Both GH3 and GC cells gave rise to clones in which about 75% of PAE was endoproteolytically processed. Mol wt distributions of PAE peptides were similar in cell extracts and medium, indicating that processing was not the result of extracellular proteases. The identity of immunoprecipitated molecules was confirmed by analysis of tryptic digests by reverse phase HPLC. Amidated forms of joining peptide were detected in the transfected cells by immunocytochemistry, adding evidence that GH-producing tumor cells can perform endoproteolysis, exoproteolysis, and alpha-amidation of the PAE precursor. One of the two forms of joining peptide from cell extracts was also shown to comigrate during reverse phase HPLC with authentic alpha-amidated mouse joining peptide. Comparisons of Northern analyses and peptide synthetic rates suggested that PAE mRNA was used for synthesis of PAE more efficiently in corticotropes than in the transfected GC cells.