gebhart/patrepeval huggingface dataset:
Comparison of Porous Bone Mineral and Biologically Active Glass in Critical‐Sized Defects (1997)

Several materials have been proposed as therapies to augment alveolar bone and to promote periodontal regeneration. However, there are an insufficient number of studies that effectively evaluated these therapies. Consequently, the purpose of this study was to compare bone regeneration promoted by porous bone mineral and biologically active glass. Unilateral critical-sized defects (CSDs) were prepared in the radii of 24 rabbits, divided evenly between 2 time periods (4 and 8 weeks) and between 2 treatment groups (porous bone mineral and biologically active glass). Evaluations consisted of clinical examinations, standardized radiography at baseline and every 2 weeks thereafter, as well as histology and histomorphometry. Data were analyzed by an unpaired Student t-test with significance established at P < or = 0.05. We determined that CSDs treated with porous bone mineral were significantly more radiopaque than biologically active glass-treated sites at both 4 and 8 weeks. Moreover, the amount of new bone was significantly greater at both 4 and 8 weeks in the porous bone mineral groups than in the biologically active glass groups. We concluded that in the rabbit radius CSD wound model, porous bone mineral appears to be more effective than biologically active glass in regenerating bone.
Sulfone-Based Electrolytes for Lithium-Ion Batteries (2002)

A series of cyclic or acyclic sulfones have been synthesized and evaluated as electrolyte solvents for lithium (ion) batteries. This report summarizes the physical and electrochemical properties of these new compounds. It is found that, while the anodic stability of these solvents is independent of the sulfone structure, i.e., no oxidative decomposition is observed before 5.5 V vs. Li in any sulfone, the structure of their alkyl substituents dictates their compatibility with graphitic anodes. In the most favorable cases, this compatibility is comparable with that found with carbonate-based electrolytes. Fluorination of alkyl groups in the sulfone seems to help in forming a stable solid electrolyte interface on the anode, while also improving the conductivity. Initial tests of these cells containing such electrolyte solvents show promising performance. © 2002 The Electrochemical Society. All rights reserved.
Advances in Gene Delivery Systems. (2011)

The transfer of genes into cells, both in vitro and in vivo, is critical for studying gene function and conducting gene therapy. Methods that utilize viral and nonviral vectors, as well as physical approaches, have been explored. Viral vector-mediated gene transfer employs replication-deficient viruses such as retro-virus, adenovirus, adeno-associated virus and herpes simplex virus. A major advantage of viral vectors is their high gene delivery efficiency. The nonviral vectors developed so far include cationic liposomes, cationic polymers, synthetic peptides and naturally occurring compounds. These nonviral vectors appear to be highly effective in gene delivery to cultured cells in vitro but are significantly less effective in vivo. Physical methods utilize mechanical pressure, electric shock or hydrodynamic force to transiently permeate the cell membrane to transfer DNA into target cells. They are simpler than viral- and nonviral-based systems and highly effective for localized gene delivery. The past decade has seen significant efforts to establish the most desirable method for safe, effective and target-specific gene delivery, and good progress has been made. The objectives of this review are to (i) explain the rationale for the design of viral, nonviral and physical methods for gene delivery; (ii) provide a summary on recent advances in gene transfer technology; (iii) discuss advantages and disadvantages of each of the most commonly used gene delivery methods; and (iv) provide future perspectives.
Deviation Control With Steerable System Lowers Well Costs in Southern Oklahoma (1991)

ABSTRACT This paper describes case histories of wells drilled primarily in two fields in Southern Oklahoma, Southwest Davis and Cottonwood Creek, which are located in an area of known deviation problems. Past operations in this "crooked hole country" have employed conventional means of drilling these problem wells, including running relatively light weights and stiff, packed hole assemblies. Ordinarily, conventional directional tools are used to direct the hole to a specified location. In an attempt to control deviation problems and increase penetration rates, a steerable system was incorporated into the drilling program for the 8-3/4″ hole size, at depths that ranged from 2000 to more than 8000 feet. While directional tools were used to direct the hole to a specified location, use of the steerable system resulted in a drastic reduction in the number of days required to drill the interval.
Determination of mortar strength using stone dust as a partially replaced material for cement and sand (2014)

Mortar is a masonry product which is matrix of concrete. It consists of binder and fine aggregate and moreover, it is an essential associate in any reinforced structural construction. The strength of mortar is a special concern to the engineer because mortar is responsible to give protection in the outer part of the structure as well as at a brick joint in masonry wall system. The purpose of this research is to investigate the compressive strength and tensile strength of mortar, which are important mechanical properties, by replacing the cement and sand by stone dust. Moreover, to minimize the increasing demand of cement and sand, checking of appropriateness of stone dust as a construction material is necessary to ensure both solid waste minimization and recovery by exchanging stone dust with cement and sand. Stone dust passing by No. 200 sieve, is used as cement replacing material and retained by No. 100 sieve is used for sand replacement. Sand was replaced by stone dust of 15%, 20%, 25%, 30%, 35%, 40%, 45% and 50% by weight of sand while cement was replaced by stone dust of 3%, 5%, and 7% by weight of cement. Test result indicates that, compressive strength of specimen mix with 35% of sand replacing stone dust and 3% of cement replacing stone dust increases 21.33% and 22.76% respectively than the normal mortar specimen at 7 and 28 days while for tensile it increases up to 13.47%. At the end, optimum dose was selected and crack analysis as well as discussion also included.
Theory of regular M-band wavelet bases (1993)

Orthonormal M-band wavelet bases have been constructed and applied by several authors. This paper makes three main contributions. First, it generalizes the minimal length K-regular 2-band wavelets of Daubechies (1988) to the M-band case by deriving explicit formulas for K-regular M-band scaling filters. Several equivalent characterizations of K-regularity are given and their significance explained. Second, two approaches to the construction of the (M-1) wavelet filters and associated wavelet bases are described; one relies on a state-space characterization with a novel technique to obtain the unitary wavelet filters; the other uses a factorization approach. Third, this paper gives a set of necessary and sufficient condition on the M-band scaling filter for it to generate an orthonormal wavelet basis. The conditions are very similar to those obtained by Cohen (1990) and Lawton (1990) for 2-band wavelets.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A 10-bit Charge-Redistribution ADC Consuming 1.9 $\mu$W at 1 MS/s (2010)

This paper presents a 10 bit successive approximation ADC in 65 nm CMOS that benefits from technology scaling. It meets extremely low power requirements by using a charge-redistribution DAC that uses step-wise charging, a dynamic two-stage comparator and a delay-line-based controller. The ADC requires no external reference current and uses only one external supply voltage of 1.0 V to 1.3 V. Its supply current is proportional to the sample rate (only dynamic power consumption). The ADC uses a chip area of approximately 115×225 μm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . At a sample rate of 1 MS/s and a supply voltage of 1.0 V, the 10 bit ADC consumes 1.9 μW and achieves an energy efficiency of 4.4 fJ/conversion-step.
A noise-shaping coder topology for 15+ bit converters (1989)

A topology for high-precision noise-shaping converters that can be integrated on a standard digital IC process is presented. This topology uses a multibit noise-shaping coder and a novel form of dynamic element matching to achieve high accuracy and long-term stability without requiring precision matching of components. A fourth-order noise-shaping D/A (digital-to-analog) conversion system using a 3-b quantizer and a dynamic element-matching internal D/A converter, fabricated in a standard double-metal 3- mu m CMOS process, achieved 16-bit dynamic range and a harmonic distortion below -90 dB. This multibit noise-shaping D/A conversion system achieved performance comparable to that of a 1-bit noise-shaping D/A conversion system that operated at nearly four times its clock rate.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Growth and differentiation of the normal mammary gland and its tumours. (1998)

The mammary glands of non-pregnant rodents and humans consist of epithelial, intermediate stem and myoepithelial cells, and these have been isolated as cell lines in vitro. Growth factors produced by the myoepithelial cells, e.g. transforming growth factor alpha (TGF alpha) and basic fibroblast growth factor (bFGF), can stimulate the growth of the intermediate stem cells in vitro. One protein, p9Ka, a calcium binding regulatory protein, arises at an early stage of the differentiation of epithelial into myoepithelial cells in vitro and is associated with the cytoskeleton; another, cathepsin D, is a protease associated with this pathway in vivo. Unlike normal glands and benign lesions, malignant mammary carcinomas of rats and humans do not contain fully differentiated myoepithelial cells, and the resultant cell lines fail to differentiate completely into myoepithelial cells. Loss of the myoepithelial cells in some human invasive carcinomas may account, in part, for compensatory changes in the malignant cells. For example, overexpression of TGF alpha/ErbB-2 receptors may compensate for a decrease in TGF alpha, whereas ectopic production of bFGF and its receptors, and of p9Ka and cathepsin D, may help in tumorigenesis and in metastasis respectively. Thus compensation for, or retention of, molecules potentially involved in growth and/or differentiation by some human invasive carcinomas may be a mechanism by which a malignancy progresses.
Death by suicide: one way to go in mammalian cellular development? (1991)

Cell deaths occur selectively in many types of tissues throughout development. These physiological deaths appear to follow an orderly process of internal cellular disintegration that is distinct from the process observed in cell death resulting from trauma. Studies of a variety of physiological cell deaths have revealed that this process appears generally to involve the active participation of the dying cell in its own death. In other words, physiological cell death seems to be a process of induced cellular self-destruction, or cell suicide. Whether a single, genetically determined mechanism is utilized in all cell suicides remains to be established. Nonetheless, while genome digestion and intracellular calcium rises are dissociable from, and thus neither necessary nor sufficient for, cell death, control of the cell cycle may be critical in all cases of induced cell suicide. It is proposed here that physiological cell death occurs through a process of abortive mitosis.
Analog Quantum Error Correction (1998)

Quantum error-correction routines are developed for continuous quantum variables such as position and momentum. The result of such analog quantum error correction is the construction of composite continuous quantum variables that are largely immune to the effects of noise and decoherence.
Genesis of Sigmoidal Dose‐Response Curve During Defibrillation by Random Shock: A Theoretical Model Based on Experimental Evidence for a Vulnerable Window During Ventricular Fibrillation (1990)

The sigmoidal dose-response curve (percent success vs shock energy) suggests a probabilistic nature of defibrillation. The mechanism is still largely unknown, however, random variation in the excitable state during ventricular fibrillation (VF) is suspected. A canine defibrillation study was designed to determine whether random variation in absolute VF voltage (AVFV) (a crude marker of number of excitable cells) was related to success of defibrillation, using a DC shock successful at the 50% level. The results were: (a) transmyocardial resistance (73.4 +/- 1.4 vs 73.6 +/- 1.5 ohms) and delivered energy (6.1 +/- 1.2 vs 6.2 +/- 1.2 joules) were similar; however, (b) AVFV 2 msec prior to DC shock was greater for successful as compared to unsuccessful attempts (0.5 +/- 0.1 vs 0.3 +/- 0.0 mV, P less than 0.01). A mathematical model was subsequently developed based on fluctuation in the number of excitable cells. Variation in the state of excitability resulted in a cyclic window potentially vulnerable to defibrillation. The vulnerable window occurred at a point when the number of excitable cells was low, i.e., a higher state of total depolarization, which was in agreement with the experimental finding. For a given VF pattern, duration of the vulnerable window was regulated by the shock energy. A larger shock energy generated a wider vulnerable window and, in turn, a higher success rate. Finally, the sigmoidal dose-response curve of defibrillation was theoretically constructed by calculating the variable chances of a random DC shock occurring either in a vulnerable window or elsewhere during VF. It is concluded that a vulnerable window susceptible to defibrillation can be demonstrated in the early stages (10 sec) of VF. The mathematical model provides a theoretical basis for the vulnerable window and helps elucidate the probabilistic nature of defibrillation.
Characterization of the Proline-Rich Region of Murine Leukemia Virus Envelope Protein (1998)

Mammalian type C retroviral envelope proteins contain a variable proline-rich region (PRR), located between the N-terminal receptor-binding domain and the more highly conserved C-terminal portion of the surface (SU) subunit. We have investigated the role of the PRR in the function of murine leukemia virus (MuLV) envelope protein. In the MuLVs, the PRR contains a highly conserved N-terminal sequence and a hypervariable C-terminal sequence. Despite this variability, the amphotropic PRR could functionally substitute for the ecotropic PRR. The hypervariable region of the PRR was not absolutely required for envelope protein function. However, truncations in this region resulted in decreased levels of both the SU and TM proteins in viral particles and increased amounts of the uncleaved precursor protein, Pr85. In contrast, the N-terminal conserved region was essential for viral infectivity. Deletion of this region prevented the stable incorporation of envelope proteins into viral particles in spite of normal envelope protein processing, wild-type levels of cell surface expression, and a wild-type ability to induce syncytia in an XC cell cocultivation assay. However, higher levels of the SU protein were shed into the supernatant, suggesting a defect in SU-TM interactions. Our data are most consistent with a role for the PRR in stabilizing the overall structure of the protein, thereby affecting the proper processing of Pr85, SU-TM interactions, and the stable incorporation of envelope proteins into viral particles. In addition, we have demonstrated that the PRR can tolerate the insertion of a peptide-binding domain, making this a potentially useful site for constructing targetable retroviral vectors.
Electrooptic sampling in GaAs integrated circuits (1986)

Electrooptic sampling has been shown to be a very powerful technique for making time-domain measurements of fast electronic devices and circuits. Previous embodiments relied on a hybrid connection between the device under test and a transmission line deposited on an electrooptic substrate such as LiTaO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> . The hybrid nature of this approach leads to device packaging difficulties and can result in measurement inaccuracies and performance degradation at very high frequencies. Since GaAs is electrooptic and an attractive material for high speed devices, we have devised an approach of direct electrooptic sampling of voltage waveforms in the host semiconductor. In this paper, we review the principles and limitations of electrooptic sampling and discuss this new noninvasive technique for electronic probing with applications to characterizing high speed GaAs circuits and devices.
Proteomic identification of novel targets regulated by the mammalian target of rapamycin pathway during oligodendrocyte differentiation (2011)

Previous work from our laboratory demonstrated that the mammalian target of rapamycin (mTOR) is active during and required for oligodendrocyte progenitor cell (OPC) differentiation. Here, we applied an iTRAQ mass spectrometry-based proteomic approach to identify novel targets of the mTOR pathway during OPC differentiation. Among the 978 proteins identified in this study, 328 (34%) exhibited a greater than 20% change (P < 0.05) in control versus rapamycin-treated cultures following 4 days of differentiation in vitro. Interestingly, 197 (20%) proteins were elevated in rapamycin-treated cultures, while 131 (13%) proteins were downregulated by rapamycin. In support of our previous data, inhibiting mTOR caused a dramatic reduction in the expression of myelin proteins. mTOR also was required for the induction of proteins involved in cholesterol and fatty acid synthesis, as well as the expression of many cytoskeletal proteins, cell signaling components, and nuclear/transcriptional regulators. Of particular interest was the identification of several critical mediators of oligodendrocyte differentiation. Specifically, mTOR activity controls the developmentally programmed upregulation of the prodifferentiation factors Fyn and Quaking, whereas the expression of the differentiation repressor Gpr17 was elevated by mTOR inhibition. These data reveal a distinct signature of mTOR-regulated protein expression during OPC differentiation.
Intermodulation Analysis of FDMA Satellite Systems Employing Compensated and Uncompensated TWT's (1982)

In a frequency-division multiple-access (FDMA) satellite communication system, the output power of the TWT (or FET) amplifier is backed from saturation to reduce the intermodulation distortion caused by AM/AM and AM/PM nonlinearities. The required amount of backoff can be considerable, wasting valuable satellite power, unless some scheme is used to compensate for these nonlinearities. A comparative study is presented for the effectiveness of three types of predistortion compensators: (1) a cuber predistortion linearizer, which is the simplest to build; (2) an AM/PM equalizer; and (3) an ideal linearizer, which is quite difficult to realize in practice.
Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides (1995)

Two synthetic peptides 31 and 32 amino acids in length were prepared as deduced from a known amino acid sequence of penicillin-binding protein 2' (PBP2') of methicillin-resistant Staphylococcus aureus. Two monoclonal antibodies were generated from fused cells of myeloma cells and splenic cells of mice immunized with the synthetic peptides. Western blot (immunoblot) analysis demonstrated specific binding of the antibodies to PBP2' of a methicillin-resistant S. aureus strain. An immunoradiometric assay was developed by using these antibodies for simple detection of PBP2'.
Immunocytochemical analysis of estrogen receptors as a predictor of prognosis in breast cancer patients: comparison with quantitative biochemical methods. (1989)

Biochemical quantitation of estrogen receptors has been used to predict prognosis in breast cancer. Immunocytochemical analysis of estrogen receptors correlates with biochemical analysis but has very few follow-up studies in the literature to validate it as a prognostic indicator. 257 patients were followed for up to 10 years (median, 6.2 years) after primary surgical treatment. Estrogen receptor analysis using both biochemical and immunocytochemical techniques was performed on their tumor specimens. Patients with positive estrogen receptor values had longer survival than patients with negative values. This was demonstrated by both methods in the first 5 years of follow-up but only by immunochemistry after 5 years. The relationship between estrogen receptor status and disease-free interval was less strong than with survival. This study demonstrates that immunocytochemical estrogen receptor analysis was of prognostic significance.
Resonant interband tunneling spin filter (2002)

We propose an InAs/GaSb/AlSb-based asymmetric resonant interband tunneling diode as a spin filter. The interband design exploits large valence band spin–orbit interaction to provide strong spin selectivity, without suffering from fast hole spin relaxation. Spin filtering efficiency is also enhanced by the reduction of tunneling through quasibound states near the zone center, where spin spitting vanishes and spin selectivity is difficult. Our calculations show that, when coupled with an emitter or collector capable of lateral momentum selectivity, the asymmetric resonant interband tunneling diode can achieve significant spin filtering in conventional nonmagnetic semiconductor heterostructures under zero magnetic field.
Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin helveticin J (1990)

Lactobacillus helveticus 481 produces a 37-kDa bacteriocin called helveticin J. Libraries of chromosomal DNA from L. helveticus were prepared in lambda gt11 and probed for phage-producing fusion proteins that could react with polyclonal helveticin J antibody. Two recombinant phage, HJ1 and HJ4, containing homologous inserts of 350 and 600 bp, respectively, produced proteins that reacted with antibody. These two phage clones specifically hybridized to L. helveticus 481 total genomic DNA but not to DNA from strains that did not produce helveticin J or strains producing unrelated bacteriocins. HJ1 and HJ4 lysogens produced beta-galactosidase fusion proteins that shared similar epitopes with each other and helveticin J. The intact helveticin J gene (hlv) was isolated by screening a library of L. helveticus chromosomal DNA in lambda EMBL3 with the insert DNA from phage HJ4 as a probe. The DNA sequence of a contiguous 3,364-bp region was determined. Two complete open reading frames (ORF), designated ORF2 and ORF3, were identified within the sequenced fragment. The 3' end of another open reading frame, ORF1, was located upstream of ORF2. A noncoding region and a putative promoter were located between ORF1 and ORF2. ORF2 could encode an 11,808-Da protein. The L. helveticus DNA inserts of the HJ1 and HJ4 clones reside within ORF3, which begins 30 bp downstream from the termination codon of ORF2. ORF3 could encode a 37,511-Da protein. Downstream from ORF3, the 5' end of another ORF (ORF4) was found. A Bg/II fragment containing ORF2 and ORF3 was cloned into pGK12, and the recombinant plasmid, pTRK135, was transformed into Lactobacillus acidophilus via electroporation. Transformants carrying pTRK135 produced a bacteriocin that was heat labile and exhibited an acitivity spectrum that was the same as that of helveticin J.