gebhart/patrepeval huggingface dataset:
Effect of the Macromolecular Metabolism of Murine Hybridoma Cells on Antibody Productivity in Batch and Fed-Batch Culture (2006)

Batch and fed-batch cultures of a murine hybridoma cell line (AFP-27) have been performed in a stirred tank reactor to estimate effect of macromolecular composition on antibody production. Macromolecular composition was found to change dynamically during batch culture of hybridoma cells possibly due to active production of RNA and protein and high metabolic rate of total cellular carbohydrates during exponential phase. Antibody synthesis from the amino acid pool is expected to compete with production of cellular proteins. Therefore, it is necessary to examine the relationship between cell growth in terms of cellular macromolecules and antibody production. A set of fed-batch cultures of a murine hybridoma cell line (AFP-27) have been performed in a stirred tank reactor to elucidate this relationship. In this study, we searched for an optimum feeding strategy by changing the target specific growth rate in fed-batch culture to give higher antibody productivity while examining the macromolecular composition. From this study, the specific production rate of cellular protein and RNA were found to have maximum rates in the fed-batch culture with a target specific growth rate of 0.035 hr1 and to decrease with target specific growth rate below 0.035 hr1 Specific antibody production rate was found to be significantly increased in the fed-batch cultures with target specific growth rate of 0.01 hr1 in which production of cellular RNA and protein were strictly limited by slow feeding of glucose and other nutrients.
Haplotype-based identification of a microsomal transfer protein marker associated with the human lifespan (2003)

We previously reported a genomewide linkage study for human longevity using 308 long-lived individuals (LLI) (centenarians or near-centenarians) in 137 sibships and identified statistically significant linkage within chromosome 4 near microsatellite D4S1564. This interval spans 12 million bp and contains approximately 50 putative genes. To identify the specific gene and gene variants impacting lifespan, we performed a haplotype-based fine-mapping study of the interval. The resulting genetic association study identified a haplotype marker within microsomal transfer protein as a modifier of human lifespan. This same variant was tested in a second cohort of LLI from France, and although the association was not replicated, there was evidence for statistical distortion in the form of Hardy-Weinberg disequilibrium. Microsomal transfer protein has been identified as the rate-limiting step in lipoprotein synthesis and may affect longevity by subtly modulating this pathway. This study provides proof of concept for the feasibility of using the genomes of LLI to identify genes impacting longevity.
Role of proline, cysteine and a disulphide bridge in the structure and activity of the anti-microbial peptide gaegurin 5 (2002)

Gaegurin 5 (GGN5) is a cationic 24-residue anti-microbial peptide isolated from the skin of a Korean frog, Rana rugosa. It contains a central proline residue and an intra-residue disulphide bridge in its C-terminus, which are common to the anti-microbial peptides found in Ranidae. We determined the solution structure of GGN5 bound to SDS micelles for the first time and investigated the role of proline, cysteine and a disulphide bridge on the structure and activity of GGN5. GGN5 adopts an amphipathic alpha-helical structure spanning residues 3-20 kinked around Pro-14, which allows the hydrophobic residues to reside in the concave helical region, and a disulphide-bridged loop-like conformation in its C-terminus. By replacement of proline with alanine (PAGGN5), a straight and rigid helix was formed in the central region and was more stable than the kinked helix. Reduction of a disulphide bridge in the C-terminus (GGN5SH) maintained the loosely ordered loop-like conformation, while the replacement of two cysteines with serines (CSGGN5) caused the C-terminal conformation to be completely disordered. The magnitude of anti-microbial activity of the peptides was closely related to their helical stability in the order PAGGN5>GGN5>GGN5SH>CSGGN5, suggesting that the helical stability of the peptides is important for anti-microbial activity. On the other hand, the significant increase of haemolytic activity of PAGGN5 implies that a helical kink of GGN5 could be involved in the selectivity of target cells. The location of GGN5 and PAGGN5, analysed using paramagnetic probes, was mainly at the surface of SDS micelles, although the location of the N-terminal region was slightly different between them.
Therapeutic interleukin-6 blockade reverses transforming growth factor-beta pathway activation in dermal fibroblasts: insights from the faSScinate clinical trial in systemic sclerosis (2018)

NCT01532869; Post-results.
Hyperglycemia and Oxidative Stress Strengthen the Association Between Myeloperoxidase and Blood Pressure (2010)

Scavenging of the vasodilator nitric oxide by myeloperoxidase activity in the vasculature may contribute to hypertension. Because hydrogen peroxide is a cosubstrate of myeloperoxidase, hyperglycemia-induced oxidative stress may strengthen the relationship between myeloperoxidase and blood pressure. We investigated this relationship and its modification by hyperglycemia and oxidative stress in a population-based cohort of elderly subjects with normal glucose metabolism (n=267), impaired glucose metabolism (n=189), and type 2 diabetes (n=290). In an age- and sex-adjusted linear regression model, plasma myeloperoxidase was positively associated with systolic blood pressure (2.10 mm Hg per 1 SD increment of myeloperoxidase [95% CI: 0.66 to 3.54]), and this association was stronger at higher levels of fasting glucose (0.61 [-1.70 to 2.93], 1.33 [-1.43 to 4.10], and 3.42 [1.01 to 5.82] for increasing tertiles of glucose) and higher plasma levels of oxidized low-density lipoprotein (0.92 [-1.31 to 3.14], 2.00 [-0.71 to 4.70], and 3.58 [0.98 to 6.19] for increasing tertiles of oxidized low-density lipoprotein). Likewise, the relationship between myeloperoxidase and blood pressure was strongest under conditions associated with oxidative stress, like obesity, low high-density lipoprotein cholesterol, metabolic syndrome, and type 2 diabetes. The strength of these associations was only marginally attenuated by adjustment for other cardiovascular risk factors. Our data demonstrate that myeloperoxidase is positively and independently associated with blood pressure, and this association is strongest in subjects with (hyperglycemia-induced) oxidative stress. These observations, together with emerging evidence that myeloperoxidase-derived oxidants contribute to the initiation and propagation of cardiovascular disease, identify myeloperoxidase as a promising target for drug development.
Simple Network Management Protocol (SNMP) Context EngineID Discovery (2008)

The Simple Network Management Protocol (SNMP) version three (SNMPv3)
requires that an application know the identifier (snmpEngineID) of the
remote SNMP protocol engine in order to retrieve or manipulate objects
maintained on the remote SNMP entity. This document introduces a
well-known localEngineID and a discovery mechanism that can be used to
learn the snmpEngineID of a remote SNMP protocol engine. The proposed
mechanism is independent of the features provided by SNMP security
models and may also be used by other protocol interfaces providing
access to managed objects. This document updates RFC 3411.
[STANDARDS-TRACK]
Electrochemical Properties of Adriamycin Adsorbed on a Mercury Electrode Surface (1984)

Abstract The electrochemical behavior of adriamycin has been studied by means of cyclic d.c. and a.c. voltammetry with a hanging mercury drop electrode, quinizarin being used as a model compound. Both adriamycin and quinizarin are strongly adsorbed on the mercury electrode with their aromatic ring planes oriented parallel to the electrode surface, and give two sets of reduction waves. The first wave, due to the quinone moieties of the adsorbed adriamycin and quinizarin, has been explained on terms of a two-step one-electron surface redox reaction. The formal standard redox potentials, semiquinone formation constants, and charge transfer rate constants of the surface redox reaction of the quinone moieties have been determined. Although the reduced form of adriamycin is not very stable chemically, it is converted to a stable and electrochemically active form for a few minutes at pH 4.54. The second wave appearing at more negative potential would be due to a kinetic or catalytic process.
Infection Assays of Tomato and Apple Fruit by the Fungal Pathogen Botrytis cinerea (2014)

Botrytis cinerea (B. cinerea) is an aggressive fungal pathogen that infects more than 200 plant species. Furthermore, the pathogen can attack fruits of some plants, such as tomato and apple. B. cinerea has become one of the model systems in molecular phytopathology because of its economic importance and sophisticated genetic operation methods. Virulence assays are very important in the study of fungal pathogenesis. This protocol details the artificial inoculation procedure of B. cinerea on tomato and apple fruits. It also can be used to analyse the virulence of postharvest fungal pathogens on other fruits, such as pear, peach, jujube and so on.
Specificity of a Mycobacterium kansasii phenolic glycolipid (mycoside A) immunoserum (1987)

The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii showed that all strains of M. kansasii and the representative strain of M. gastri formed the antigen, whereas none of the remaining species (including M. leprae) formed it. Consequently, it was found that the antiserum was highly specific and useful for diagnostic purposes.
Molecular Targeting and Treatment of Composite EGFR and EGFRvIII-Positive Gliomas Using Boronated Monoclonal Antibodies (2008)

Both EGFRvIII and wild-type EGFR tumor cell populations must be targeted using a combination of BD-cetuximab and BD-L8A4. Although in vitro C225 recognized both receptors, in vivo it was incapable of delivering the requisite amount of (10)B for BNCT of EGFRvIII-expressing gliomas.
Modalities for the assessment of burn wound depth. (2006)

Of the depth assessment modalities currently used in clinical practice, LDI and ICG video angiography offer the best data-supported estimates of accuracy. Until the future of new modalities unfolds, a combination of clinical evaluation and another modality--thermography, biopsy, or, ideally, ICG video angiography or LDI--is advised to best assess the depth of acute burn wounds.
Human Leukocyte Antigen (HLA) Class I and II Polymorphism in Iranian Healthy Population from Yazd Province. (2017)

The major histocompatibility complex (MHC) genes are the most polymorphic loci in the human genome and have been widely studied in various populations and ethnic groups. Investigations into the HLA genes and proteins have been useful tool for anthropological, transplantation and disease association studies. The polymorphism of the HLA class I (A, B, C) and class II (DRB1, DQA1, DQB1) genes were investigated in 90 unrelated Iranian individuals from Yazd province located in the center of Iran using sequence-specific primers (PCR-SSP). Allele and haplotype frequencies, expected/observed heterozygosity, unbiased expected heterozygosity, number of effective alleles, deviations from Hardy-Weinberg (HW) equilibrium and genetic diversity were computed. A total of 23, 48, 23, 24, 13 and 16 alleles for HLA-A, -B,-C, -DRB1, -DQA and -DQB loci were determined, respectively in the population study. The most frequent allele identified in this study were A*02:01 (18.889%), HLA-B* 51:01 (12.778%), HLA-C* 12:03 (17.033%), HLA-DRB* 11 (24.4%), HLA-DQA* 05:05 (20.55%) and HLA-DQB*03:01 (22.8%).Furthermore, the most frequent 3-locus haplotypes were DRB*11-DQA*05:01-DQB*03:01 (21.1%), HLA-A*02:01- B *50:01- DRB*07:01 (4.9%) and A*26:01 -B* 38:01 -C*12:03(5%). The most 4-locus haplotype were A*11:01 -B* 52:01 -C*12:03 -DRB!*15(2.5%) and A*02:01 -B* 50:01 -DRB1*07:01 -DQB1*02:01(4.5%). The heterozygosity of the study population was confirmed the expected value and not deviated from Hardy-Weinberg equilibrium for all loci (p>0.05). Our study shows a close relatedness between Yazd population and other ethnic group of Iran despite some differences, which may be due to admixture of each one of these groups with each other or foreigner subpopulations during centuries. Moreover, the results of this study suggest that the Iranian population from Yazd province is in close vicinity with the Caucasians populations and far from the Korean and Japanese populations.
Trophic effects of basic fibroblast growth factor (bFGF) on differentiated oligodendroglia: A mechanism for regeneration of the oligodendroglial lineage (1993)

We have investigated the effect of basic fibroblast growth factor (bFGF) on the proliferation and phenotype of differentiated oligodendroglia. Using primary cell cultures enriched in oligodendrocytes but containing few O2A-oligodendrocyte progenitor cells, we demonstrate that bFGF treatment greatly increases the proportion of O2A cells while decreasing the proportion of galactocerebroside +(GalC+), myelin basic protein +(MBP+) oligodendrocytes, and the steady state levels of MPB mRNA. Complement mediated cell lysis experiments using the A2B5 antibody to deplete existing O2A cells or the R-Mab antibody to deplete existing oligodendroglia show that bFGF elicits a rapid increase in the number of O2A cells in cultures previously depleted of O2A cells, but does not cause an early increase in O2A cells in cultures from which oligodendroglia had been removed, indicating that the oligodendrocytes are the source of the newly recruited O2A cells. This bFGF-mediated transition from oligodendrocyte to O2A cells occurs with a time course similar to the bFGF-induced increase of the proliferation rate of the GalC+ oligodendrocytes. Studies with purified, passaged cells of the oligodendroglial lineage show that bFGF augments oligodendroglial dedifferentiation and proliferation in chronologically adult oligodendrocytes and in the virtual absence of other cell types. We have thus demonstrated that mature oligodendrocytes are induced by bFGF to dedifferentiate and proliferate, suggesting a mechanism for regeneration of the oligodendroglial lineage following demyelinating disease.
Replacement of the p16/CDKN2 gene suppresses human glioma cell growth. (1995)

The p16/CDKN2 gene has many features of a growth suppressor gene: it maps to 9p21, a frequent region of loss of heterozygozity in a variety of tumor types; it encodes an inhibitor of cyclin-dependent kinase 4; and its homozygous deletion is common in tumor-derived cell lines. However, the lower frequency of alteration of the gene in primary tumor tissue as compared to the cognate tumor cell lines has brought this interpretation into question. We have assessed the growth suppressive function of p16/CDKN2 by gene transfer. The introduction of full-length p16/CDKN2 cDNA caused marked growth suppression in p16/CDKN2-null human glioma cells, but was without significant effect in those cells with endogenous wild-type p16/CDKN2 alleles. These results provide functional evidence in support of the hypothesis that the p16/CDKN2 gene is a functional growth suppressor gene, at least in gliomas.
Units in Skew Fields (2000)

This book is devoted to a study of the unit groups of orders in skew fields, finite dimensional and central over the rational field; it thereby belongs to the field of noncommutative arithmetic. Its p
Effect of CO 2 Insufflation on the Number and Behavior of Air Microemboli in Open-Heart Surgery (2004)

Insufflation of CO2 into the thoracic wound markedly decreases the incidence of microemboli.
Transitional Flow Phenomena in Concentric Annuli (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTransitional Flow Phenomena in Concentric AnnuliR. W. Hanks and W. F. BonnerCite this: Ind. Eng. Chem. Fundamen. 1971, 10, 1, 105–113Publication Date (Print):February 1, 1971Publication History Published online1 May 2002Published inissue 1 February 1971https://pubs.acs.org/doi/10.1021/i160037a018https://doi.org/10.1021/i160037a018research-articleACS PublicationsRequest reuse permissionsArticle Views299Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Overexpression of Jagged-1 and Its Intracellular Domain in Human Mesenchymal Stromal Cells Differentially Affect the Interaction with Hematopoietic Stem and Progenitor Cells (2013)

Mesenchymal stromal cells (MSC) are an important component of the bone marrow microenvironment. Notch ligands expressed by MSC are known to play a regulatory role for hematopoietic stem and progenitor cells (HSPC) and in support of bone marrow homeostasis. While the role of Notch signaling in HSPC, their progeny, and MSC has been relatively well studied, little is known about the Notch-independent regulatory impact of Notch ligands on MSC themselves. In the present study, we used genetically engineered bone marrow-derived human MSC to study the function role of Jagged-1 and the Jagged-1 intracellular domain (JICD) with regard to the interaction with HSPC. We demonstrate that Jagged-1 in human MSC undergoes cleavage to produce an intracellular domain that translocates into the nucleus. JICD but not Jagged-1 overexpression was associated with an increased expression of stromal cell-derived factor-1. Short-term co-culture (7 days) of HSPC with JICD-overexpressing MSC, but not with Jagged-1-overexpressing MSC, led to increased proliferation of CD34(+) progenitors. In contrast, long-term co-culture of HSPC with Jagged-1-overexpressing MSC (up to 6 weeks) led to a significantly better support of cobblestone area-forming cells and long-term culture-initiating cells (LTC-ICs) compared with JICD-overexpressing MSC. Taken together, results of this study indicate that full-length Jagged-1 and JICD have differential effects on MSC and on their interaction with HSPC ex-vivo. JICD-overexpressing MSC induce proliferation of HSPCs in short-term culture at the expense of immature precursors (LTC-ICs), whereas Jagged-1 overexpressing MSC favor LTC-IC formation.
Reduction of intraocular pressure by topical administration of an inhibitor of the Rho-associated protein kinase (2001)

The ROCK inhibitor reduced intraocular pressure in rabbits by topical instillation. The inhibitor relaxed the excised ciliary muscle which was previously constricted by carbachol suggesting that the inhibitor acts to increase the uveoscleral outflow. Our results suggest that the ROCK inhibitor is a promising treatment for glaucoma therapy in the next generation.
Immunophenotypic differentiation patterns of normal hematopoiesis in human bone marrow: Reference patterns for age‐related changes and disease‐induced shifts (2004)

Although alternative immunostainings can be used (e.g., including NK- and T-cell markers), we feel that the selected six stainings represent a comprehensive and easy screening phase for quick identification of shifts in the composition of the studied differentiation lineages, reflecting age-related changes or disease-induced BM abnormalities.
Normal and malignant human myelocytic and monocytic cells identified by monoclonal antibodies. (1982)

Two cytotoxic IgM monoclonal antibodies reactive with human cells of myeloid origin are described. Each antibody was tested for reactivity with normal and malignant cells of myeloid origin by using immunofluorescent and cytotoxic assays. Antibody 5F1 defines a determinant expressed by peripheral blood monocytes and platelets. Antibody 1G10 detects a determinant expressed in high concentration on granulocytes and in low concentration on a subset of monocytes. Neither antibody reacts with peripheral blood lymphocytes, erythrocytes, or thymocytes. In bone marrow, 5F1 stains monocytic and nucleated erythrocytic cells, whereas 1G10 stains cells of the granulocytic lineage. Each antibody also reacts with subsets of acute nonlymphocytic leukemia cells. These reagents should be useful in delineating stages of normal and malignant myeloid differentiation.
Electronic transport properties of amorphous indium-gallium-zinc oxide semiconductor upon exposure to water (2008)

The effect of water exposure on amorphous indium-gallium-zinc oxide (a-IGZO) semiconductors was reported. It was found that water can diffuse in and out of the a-IGZO film, reversibly affecting the transistor properties. Two competing mechanisms depending on the thickness of the active channel were clarified. The electron donation effect caused by water adsorption dominated for the thicker a-IGZO films (⩾100nm), which was manifested in the large negative shift (&amp;gt;14V) of the threshold voltage. However, in the case of the thinner a-IGZO films (⩽70nm), the dominance of the water-induced acceptorlike trap behavior was observed. The direct evidence for this behavior was that the subthreshold swing was greatly deteriorated from 0.18V/decade (before water exposure) to 4.4V/decade (after water exposure) for the thinnest a-IGZO films (30nm). These results can be well explained by the screening effect of the intrinsic bulk traps of the a-IGZO semiconductor.
The Clinician Assessment of Fluctuation and the One Day Fluctuation Assessment Scale (2000)

Both scales are useful instruments for the clinical assessment of fluctuation in dementia.
Annotated reference list agents (2002)

The agents described in the article were used to create a system which does most of the creation, addition, and maintenance of an annotated reference list page. This system has been used to maintain a telecommunications Web page for the last two years. The strength of this system is that it automatically locates a large number of potential links which may be included in the Web page, and uses a fairly fast method of interfacing with the list maintainer to verify links before their inclusion. The paper discusses the implementation of agents to automate the task of maintaining an annotated reference list.