gebhart/patrepeval huggingface dataset:
在犬的普鲁斯病的 seroprevalence 学习的 Brucella canis 和他们的使用的抗原的免疫化学的描述 (2011)

Objective:To explore immunochemical characterization of antigens of Brucella canis(B. canis),and the use in seroprevalence study of canine brucellosis.Methods:External hot phosphate buffer saline extract(HPBSE) and internal sonicated(SA) antigens were prepared from B.canis strain MEX 51 and imniunochemically characterized.These antigens were used to test 527 serum samples of dogs by 2-mercaptoethanol-tubc agglutination test(2 ME-TAT), agar gel immunodiffusion test(AGID).dot-ELISA and indirect enzyme-linked immunosorbent assay(I-ELISA) to assess the seroprevalence of canine brucellosis.Results:The protein content of HPBSE and SA antigens was 0.387 mg/ml.and 0.195 mg/mL,respectively,whereas carbohydrate content was 0.174 mg/mL and 0.150 mg/mL,respectively.The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(12.5%) of HPBSE and SA,revealed 6 and 8 visible peptide bands ranging from 18-80 kDa and 12-45 kDa,respectively.Western blot analysis showed immunodominant bands of MW 12.28.39 and 45 kl)a for HPBSE and 20-24 kl)a for SA. The AGII) revealed HPBSE as more specific antigen than SA but both I-ELISA and dot-ELISA indicated SA antigen to be more specific and reliable than HPBSE.The seroprevalence of canine brucellosis was 2.27%by 2ME-TAT.1.5%by AGID.3.03%by dot-ELISA and 16.12%by I-ELISA. Conclusions:On the basis of the results of present study,we concluded that HPBSE is suitable antigen for AGID,which is more specific:whereas SA antigen is suitable for I-ELISA,which is highly sensitive.Therefore,initial screening of serum samples should be carried out by I-ELISA followed by confirmation with AGID.
Correlation Analysis between Electrocardiography (ECG) and Photoplethysmogram (PPG) Data for Driver’s Drowsiness Detection Using Noise Replacement Method (2017)

The aim of this study is to investigate a noise handling method that provides high correlation between Electrocardiography(ECG) and Photoplethysmogram(PPG) data. This issue is important in detecting driver’s drowsiness. So far, there have been many studies to estimate the driver’s drowsiness based on heart rate variability (HRV), by examining such features such as power spectral density (PSD) from ECG data. However, since the ECG data is obtained from heart’s electrical signals through the skin’s electrodes, ECG measuring instruments are inconvenient to wear in real-life driver situations. On the other hand, with the development of PPG sensors, it becomes now easy to get HRV data through smart bands which are more convenient to wear in driving situations. But the PPG data from smart bands tend to have more noise than ECG data. Thus, handling such noises is of great significance to adopt existing ECG-based methods for PPG data in driver’s fatigue estimation. In this study, we propose a noise replacement method that identifies noises and substitutes them with appropriate values, not filtering out noises. From experiments, we observed that our noise replacement method enables us to obtain the improved correlation in PSD between ECG data and PPG data, compared to noise filtering method. This result implies that the noise replacement method may be a more effective way to handle PPG data for driver safety monitoring.
Efficient removal of uracil from G.U mispairs by themismatch-specific thymine DNA glycosylase from HeLa cells. (1994)

The uracil DNA glycosylases (EC 3.2.2.3) characterized to date remove uracil from DNA irrespective of whether it is base paired with adenine or mispaired with guanine in double-stranded substrates or whether it is found in single-stranded DNA. We report here the characterization of uracil glycosylase activity that can remove the base solely from a mispair with guanine. It does not recognize uracil either in A.U pairs or in single-stranded substrates. The enzyme, a 55-kDa polypeptide, was previously characterized as a mismatch-specific thymine DNA glycosylase and was thought to be responsible solely for the correction (to G.C) of G.T mispairs that arise as a result of spontaneous hydrolytic deamination of 5-methylcytosine to thymine. Given the broader substrate specificity of the enzyme (in addition to uracil and thymine, the protein can also remove 5-bromouracil from mispairs with guanine), we propose that its biological role in vivo may also include the correction of a subset of G.U mispairs inefficiently removed by the more abundant ubiquitous uracil glycosylases, such as those arising from cytosine deamination in G+C-rich regions of the genome.
Therapeutic vaccines (2012)

Personalized therapy is directed at obtaining maximal therapeutic effect on diseased tissue with minimal off-target side effects. Many classes of therapeutics have attempted to reach this ideal, only to fall well short. Therapeutic vaccines represent a novel class of therapies that can induce a dynamic immune response that, in theory, can continue to adapt and expand following initiation of vaccination. This adaptability, through epitope spreading or antigen cascade, can continuously refine a therapeutic immune response, making it more relevant to the patient's tumor. This active, dynamic, iterative process can continue long after the vaccine course has been completed. Recent clinical trials have provided further insight into the clinical activity of therapeutic vaccines, and offer guidance on clinical expectations following vaccine. The ongoing active sculpting of the immune response, along with the lack of significant side effects, uniquely positions therapeutic vaccines as perhaps the ultimate in personalized therapy.
Prevention of Neonatal Respiratory Distress Syndrome by Tracheal Instillation of Surfactant: A Randomized Clinical Trial (1985)

With a randomized clinical trial, the possibility was assessed that a tracheal instillation of pulmonary surfactant prior to the first breath might prevent the development of some of the signs of neonatal respiratory distress syndrome. Of the 72 infants in the trial, all born at a gestational age of less than 30 weeks, 39 received 3 or 4 mL of surfactant, prepared from the lipids extracted from calf lung lavage. The treatment resulted in a significantly improved gas exchange during the first 72 hours of life. On the average, the arterial/alveolar PO2 ratio was 0.15 higher for the treated infants, and only about half as much extra oxygen had to be supplied. The respiratory support (peak inspiratory pressure X frequency) could be lowered significantly. Pulmonary interstitial emphysema occurred in 13 of the 33 control infants, but in only three of the 39 treated infants. Six of the control infants died in the neonatal period, but only one treated infant died. It is concluded that surfactant supplementation prior to the first breath is feasible and is of value as protection against the respiratory distress syndrome and the negative effects of hypoxia and ventilatory support.
Catalytic ring-closing metathesis of functionalized dienes by a ruthenium carbene complex (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic ring-closing metathesis of functionalized dienes by a ruthenium carbene complexGregory C. Fu, SonBinh T. Nguyen, and Robert H. GrubbsCite this: J. Am. Chem. Soc. 1993, 115, 21, 9856–9857Publication Date (Print):October 1, 1993Publication History Published online1 May 2002Published inissue 1 October 1993https://pubs.acs.org/doi/10.1021/ja00074a085https://doi.org/10.1021/ja00074a085research-articleACS PublicationsRequest reuse permissionsArticle Views4937Altmetric-Citations481LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Optimal parameters for retinal illumination and imaging in fundus cameras (2008)

A fundus camera is a complex optical system for imaging the retina of the eye. Designing a fundus camera requires the combination of an imaging system and an illumination system to share common optics. This combination of systems results in the need to find an optimal balance between imaging and illuminating the retina. We present a series of parameters and methods used to optimize the illumination system of a fundus camera while maintaining excellent image quality.
Query processing techniques for solid state drives (2009)

Solid state drives perform random reads more than 100x faster than traditional magnetic hard disks, while offering comparable sequential read and write bandwidth. Because of their potential to speed up applications, as well as their reduced power consumption, these new drives are expected to gradually replace hard disks as the primary permanent storage media in large data centers. However, although they may benefit applications that stress random reads immediately, they may not improve database applications, especially those running long data analysis queries. Database query processing engines have been designed around the speed mismatch between random and sequential I/O on hard disks and their algorithms currently emphasize sequential accesses for disk-resident data.
A Conserved Gene Cluster Rules Anaerobic Oxidative Degradation of l -Ornithine (2009)

For the ornithine fermentation pathway, described more than 70 years ago, genetic and biochemical information are still incomplete. We present here the experimental identification of the last four missing genes of this metabolic pathway. They encode L-ornithine racemase, (2R,4S)-2,4-diaminopentanoate dehydrogenase, and the two subunits of 2-amino-4-ketopentanoate thiolase. While described only for the Clostridiaceae to date, this pathway is shown to be more widespread.
Wafer Bonding: A Flexible Approach to Materials Integration (2000)

Wafer bonding, also termed direct bonding or fusion bonding, has increasingly become a technology of choice for materials integration in various areas of microelectronics, optoelectronics, and microelectromechanical systems (MEMS). The Electrochemical Society has played an important role in furthering this new technology by organizing a biannual series of symposia on this subject starting in 1991. In the present article we will describe the status of this technology, and mention major accomplishments as well as challenges and opportunities for future research and applications. We will concentrate on the basic principles involved, rather than on detailed descriptions of applications. Because the literature on wafer bonding has become rather extensive, it is not our goal to be exhaustive but rather to stress aspects which are important or just interesting in our own somewhat personal view.
Low-jitter process-independent DLL and PLL based on self-biased techniques (1996)

Delay-locked loop (DLL) and phase-locked loop (PLL) designs based upon self-biased techniques are presented. The DLL and PLL designs achieve process technology independence, fixed damping factor, fixed bandwidth to operating frequency ratio, broad frequency range, input phase offset cancellation, and, most importantly, low input tracking jitter. Both the damping factor and the bandwidth to operating frequency ratio are determined completely by a ratio of capacitances. Self-biasing avoids the necessity for external biasing, which can require special bandgap bias circuits, by generating all of the internal bias voltages and currents from each other so that the bias levels are completely determined by the operating conditions. Fabricated in a 0.5-/spl mu/m N-well CMOS gate array process, the PLL achieves an operating frequency range of 0.0025 MHz to 550 MHz and input tracking jitter of 384 ps at 250 MHz with 500 mV of low frequency square wave supply noise.
Purification and characterization of a novel protein from bovine aorta that inhibits coagulation (1988)

A novel inhibitor of blood coagulation has been isolated from the intima of bovine aorta. The inhibitor, vascular anticoagulant (VAC), has been purified to an active fraction that contains two Coomassie-blue-staining bands (Mr = 34,000 and Mr = 32,000, as judged by sodium dodecyl sulfate/polyacrylamide electrophoresis). Both bands are single-chain proteins, having no glycoprotein features. Furthermore, they do not contain any detectable 4-carboxyglutamic acid residues. Both proteins have an identical isoelectric pH of approximately 4.5. VAC binds in the presence of calcium ions to a bilayer consisting of 20% dioleoylglycerophosphoserine and 80% dioleoylglycerophosphocholine with a Kd = 6 nM. The binding is dependent on the calcium concentration: half-saturation of binding occurs at a calcium concentration of 0.8 mM. The binding is completely reversible with EDTA. Furthermore the phospholipid/VAC ratio at saturation was n = 112 and n = 32 mol/mol for 0.5 mM Ca2+ and 2 mM Ca2+, respectively. Binding does not occur between VAC and pure dioleoylglycerophosphocholine. In a system with purified coagulation factors VAC inhibits the activation of prothrombin by factor Xa and calcium only in the presence of negatively charged phospholipids. VAC decreases the Vmax and increases the Km of the factor-Xa-catalyzed prothrombin activation. Based on these results, we conclude that we have purified from bovine aortic intima an anticoagulant protein, which exerts its activity through a calcium-dependent binding to negatively charged phospholipids, and thus interferes with the assembly of prothrombinase on the phospholipid surface.
Early growth of environmental plantings in relation to site and management factors (2012)

Summary In extensively cleared areas of temperate Australia, large‐scale mixed‐species environmental revegetation is often recommended to address land and water degradation in rural landscapes. Although survival and growth are central to the success of revegetation, there is very limited information on this aspect of practice. We investigated how site and management practices influence early growth by surveying 29 planted or direct‐seeded sites, covering ages from 3–6 years, in north central Victoria, Australia. We measured heights and crown widths of trees and shrubs at each site and estimated average growth over time at the scale of: (i) individual trees or shrubs (mean height increment) and (ii) stand (vegetation cover increment and a ‘height integral’ increment variable, which integrates differences in both height and stand density). Relationships between growth increment variables and site factors, including climate, site preparation and land‐use history, were investigated to identify the main factors influencing early growth rates. Mean annual increment in height of individual trees was significantly higher for trees established from tubestock than those from direct seeding. Multiple linear regression models for two growth variables, cover increment and height integral increment, explained 61% and 38% of the variation, respectively, but with high uncertainty. Ploughing, pre‐establishment weed control and fertiliser addition improved growth rates. Revegetation method had a significant effect on early growth; at the scale of individuals, growth rates were higher for planted tubestock than for direct seeding, while at the stand scale, growth rates were higher for direct seeding than for planted tubestock.
The Guanine Nucleotide Exchange Factor Tiam1 Affects Neuronal Morphology; Opposing Roles for the Small GTPases Rac and Rho (1997)

The invasion-inducing T-lymphoma invasion and metastasis 1 (Tiam1) protein functions as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac1. Differentiation-dependent expression of Tiam1 in the developing brain suggests a role for this GEF and its effector Rac1 in the control of neuronal morphology. Here we show that overexpression of Tiam1 induces cell spreading and affects neurite outgrowth in N1E-115 neuroblastoma cells. These effects are Rac-dependent and strongly promoted by laminin. Overexpression of Tiam1 recruits the alpha 6 beta 1 integrin, a laminin receptor, to specific adhesive contacts at the cell periphery, which are different from focal contacts. Cells overexpressing Tiam1 no longer respond to lysophosphatidic acid- induced neurite retraction and cell rounding, processes mediated by Rho, suggesting that Tiam1-induced activation of Rac antagonizes Rho signaling. This inhibition can be overcome by coexpression of constitutively active RhoA, which may indicate that regulation occurs at the level of Rho or upstream. Conversely, neurite formation induced by Tiam1 or Rac1 is further promoted by inactivating Rho. These results demonstrate that Rac- and Rho-mediated pathways oppose each other during neurite formation and that a balance between these pathways determines neuronal morphology. Furthermore, our data underscore the potential role of Tiam1 as a specific regulator of Rac during neurite formation and illustrate the importance of reciprocal interactions between the cytoskeleton and the extracellular matrix during this process.
A suite of Gateway® cloning vectors for high‐throughput genetic analysis in Saccharomyces cerevisiae (2007)

In the post-genomic era, academic and biotechnological research is increasingly shifting its attention from single proteins to the analysis of complex protein networks. This change in experimental design requires the use of simple and experimentally tractable organisms, such as the unicellular eukaryote Saccharomyces cerevisiae, and a range of new high-throughput techniques. The Gateway system has emerged as a powerful high-throughput cloning method that allows for the in vitro recombination of DNA with high speed, accuracy and reliability. Two Gateway-based libraries of overexpression plasmids containing the entire complement of yeast open reading frames (ORFs) have recently been completed. In order to make use of these powerful resources, we adapted the widely used pRS series of yeast shuttle vectors for use in Gateway-based cloning. The resulting suite of 288 yeast Gateway vectors is based upon the two commonly used GPD and GAL1 promoter expression systems that enable expression of ORFs, either constitutively or under galactose-inducible conditions. In addition, proteins of interest can be fused to a choice of frequently used N- or C-terminal tags, such as EGFP, ECFP, EYFP, Cerulean, monomeric DsRed, HA or TAP. We have made this yeast Gateway vector kit available to the research community via the non-profit Addgene Plasmid Repository (http://www.addgene.org/yeast_gateway).
Ultra-fast 3D filtered backprojection on commodity graphics hardware (2005)

Recent efforts in cone-beam scanner technology have focused on developing interactive scanning capabilities, for example, to enable image-guided surgical interventions or real-time diagnosis with time-varying data. However, apart from a fast scanner these applications also require a fast reconstruction algorithm to match. The filtered backprojection algorithm devised by Feldkamp, Davis, and Kress is the most widely used algorithm for 3D reconstruction from cone-beam projections, and it is the algorithm with the lowest complexity. Yet, pure software implementations have difficulties to process the data at the speeds required for real-time scanning. One option is to utilize expensive and rare custom boards for this purpose. We describe an alternative solution, which is inexpensive, uses readily available PC graphics hardware boards, and provides the desired performance at the quality required.
Autoantibodies in Autism Spectrum Disorders (ASD) (2007)

Autism spectrum disorders (ASD) are a heterogeneous group of neurodevelopmental disorders defined behaviorally by abnormalities in social, verbal, and nonverbal communication. The etiologies of ASD are unknown, likely to be the result of a variety of numerous genetic, neurological, environmental, and immunological interactions that lead to a general behavioral phenotype defined as ASD. This review will focus on the various immune system anomalies, in particular, autoantibodies, which have been reported in subjects with ASD. In addition, we will discuss recent studies performed by our group concerning the presence of autoantibodies directed against neural antigens, which are observed in patients with ASD.
Investigation of the nature of the methionine–π interaction in β‐hairpin peptide model systems (2004)

There are frequent contacts between aromatic rings and sulfur atoms in proteins. However, it is unclear to what degree this putative interaction is stabilizing and what the nature of the interaction is. We have investigated the aryl-sulfur interaction by placing a methionine residue diagonal to an aromatic ring on the same face of a beta-hairpin, which places the methionine side chain in close proximity to the aryl side chain. The methionine (Met)-aryl interaction was compared with an equivalent hydrophobic and cation-pi interaction in the context of the beta-hairpin. The interaction between phenylalanine (Phe), tryptophan (Trp), or cyclohexylalanine (Cha) and Met stabilized the beta-hairpin by -0.3 to -0.5 kcal mole(-1), as determined by double-mutant cycles. The peptides were subjected to thermal denaturations that suggest a hydrophobic driving force for the interactions between Met and Trp or Cha. The observed interaction of Met or norleucine (Nle) with Trp or Cha are quite similar, implying a hydrophobic driving force for the Met-pi interaction. However, the thermodynamic data suggest that there may be some differences between the interaction of Met with Trp and Phe and that there may be a small thermodynamic component to the Met...Phe interaction.
Evaluation of bone turnover and osteoclastic cytokines in early rheumatoid arthritis treated with alendronate. (1999)

Our study demonstrates that alendronate reduces bone turnover in early RA and may have a possible antiarthritic effect. Because of the mild early form of the disease in the study cohort, further evaluations are required to confirm this effect.
Functional glass surface displaying a glutamyl donor substrate for transglutaminase‐mediated protein immobilization (2010)

A chemically modified glass surface displaying a glutamyl donor substrate peptide (Z-QG) was developed for microbial transglutaminase (MTG)-mediated immobilization of recombinant proteins tagged with an MTG-reactive lysine-containing substrate peptide (K-tag). To evaluate the surface modification conditions affecting the enzymatic protein immobilization, we employed an amino-modified 96-well glass plate as a base and prepared three types of glass surfaces displaying Z-QG. Validation of the Z-QG modified glass surfaces with recombinant enhanced green fluorescent proteins revealed that the insertion of a di(ethylene glycol) linker between the terminal Z-QG moiety and the base not only enhances enzymatic protein immobilization efficiency but also decreases nonselective protein adsorption. A bacterial alkaline phosphatase fused with a K-tag at the N terminus was also successfully immobilized to the designed glass surface, suggesting that the chemically modified glass surface displaying a spatially controlled glutamyl donor substrate is a potential platform for MTG-mediated fabrication of protein-based solid biomaterials.