gebhart/patrepeval huggingface dataset:
Battery management systems (BMS) for increasing battery life time (1999)

The life time of the battery depends of many different parameters. One parameter set is the internal battery parameters which are influenced by the battery manufacturers, and the second set is the external battery parameters which are influenced by the battery users. The external parameter can have an enormous influence on the life time. By use of a battery management system (BMS) it is possible to control the external parameters with the target to extend battery lifetime. This paper describes a BMS consisting of a data acquisition system, battery state calculation, electrical management, thermal management, safety/supervisory management, and communication. If the functions of a BMS are optimized to the used battery type and application, it is possible to extend battery lifetime.
A Concise Synthesis of a Novel Antiangiogenic Tyrosine Kinase Inhibitor (2004)

An efficient synthesis of the potent KDR inhibitor 3-[5-[[4-(methylsulfonyl)-1-piperazinyl]methyl]-1H-indole-2-yl]quinolin-2(1H)-one (1) is described. The process features a noncryogenic indole boronation and a dicyclohexylamine-mediated Suzuki coupling.
Assessment of SAGD Well Configuration Optimization in Lloydminster Heavy Oil Reserve (2012)

Abstract Large quantity of heavy oil resources are present in variety of complex thin reservoirs in Lloydminster area which are situated in east-central Alberta and west-central Saskatchewan. Primary depletion and waterflooding are the principal recovery techniques. Although these techniques work, the recovery factors remain low and large volumes of oil are left unrecovered when these methods have been exhausted. Because of the large quantities of sand production, many of these reservoirs end up with a network of wormholes that makes most of the displacement type enhanced oil recovery techniques inapplicable. Because of these high conductivity channels, only gravity drainage based techniques have a good chance of success. Among the applicable methods in Lloydminster area, SAGD has not received adequate attention, mostly due to the notion that heat loss in thin reservoirs would make the process uneconomical. While this may be true, the limiting reservoir thickness for SAGD under varying conditions has not been established. These reservoirs contain light oil with sufficient mobility. Therefore the communication between the SAGD well pairs is no longer a hurdle. This opens up the possibility of increasing the distance between the two wells and introducing elements of steamflooding into the process in order to compensate for the small thickness of the reservoir. The main objective of this study was to evaluate the effect of well configuration on SAGD performance and develop a methodology for enhancement of the SAGD performance through optimizing the well configurations for Lloydminster type of reservoir. A new well configuration was able to significantly improve the application of SAGD in thin reservoirs of Lloydminster. It provided high RF at reasonable cSOR. The effects of some common Lloydminster reservoir characteristics, which are problematic for the SAGD process (such as initial gas saturation, bottom water, and gas-cap) were investigated for the most promising well configuration.
Nanoscale three-dimensional single particle tracking (2011)

Single particle tracking (SPT) in biological systems is a quickly growing field. Many new technologies are being developed providing new tracking capabilities, which also lead to higher demands and expectations for SPT. Following a single biomolecule as it performs its function provides quantitative mechanistic information that cannot be obtained in classical ensemble methods. From the 3D trajectory, information is available over the diffusional behavior of the particle and precise position information can also be used to elucidate interactions of the tracked particle with its surroundings. Thus, three-dimensional (3D) SPT is a very valuable tool for investigating cellular processes. This review presents recent progress in 3D SPT, from image-based techniques toward more sophisticated feedback approaches. We focus mainly on the feedback technique known as orbital tracking. We present here a modified version of the original orbital tracking in which the intensities from two z-planes are simultaneously measured allowing a concomitant wide-field imaging. The system can track single particles with a precision down to 5 nm in the x-y plane and 7 nm in the axial direction. The capabilities of the system are demonstrated using single virus tracing to follow the infection pathway of Prototype Foamy Virus in living cells.
Motor learning in Ts65Dn mice, a model for Down syndrome (2000)

Ts65Dn mice are a genetic model for Down syndrome. Both individuals with Down syndrome and Ts65Dn mice have reduced cerebellar volumes and the cerebellum is involved in motor learning. Conflicting results have been reported on the motor learning abilities of Ts65Dn mice, which may be related to procedural differences between the motor learning tasks used in different laboratories and/or variability in phenotype because of the segregating background on which the mice are maintained. In this study, we examined learning in three types of motor tasks (peg running, accelerating rotorod, and rotating rod) which were initially easy for mice and gradually increased in difficulty. Ts65Dn mice learned the peg running task as well as controls, and learned the accelerating rotorod and rotating rod tasks as well as, and even better than, controls. These data indicate that Ts65Dn mice are not impaired in motor learning.
The structural basis for the interaction between L-tryptophan and the Escherichia coli trp aporepressor. (1987)

We have employed equilibrium dialysis to help study the mechanism by which the unliganded Escherichia coli trp aporepressor is activated by L-tryptophan to the liganded trp repressor. By measuring the relative affinity of L-tryptophan and various tryptophan analogues for the co-repressor's binding site, we have estimated the extent to which each of the functional groups of L-tryptophan contributes to the liganding process and discuss their role in the context of the crystal structures of the trp repressor and aporepressor. We have found that the indole ring and alpha carboxyl group of L-tryptophan are mainly responsible for its affinity to the aporepressor. The alpha amino group, however, has a small negative contribution to the affinity of L-tryptophan for the aporepressor which may be associated with its essential role in operator-specific binding.
Beyond bloom filters (2006)

Many networking applications require fast state lookups in a concurrent state machine,which tracks the state of a large number of flows simultaneously.We consider the question of how to compactly represent such concurrent state machines. To achieve compactness,we consider data structures for Approximate Concurrent State Machines (ACSMs)that can return false positives,false negatives,or a "don 't know "response.We describe three techniques based on Bloom filters and hashing,and evaluate them using both theoretical analysis and simulation.Our analysis leads us to an extremely efficient hashing-based scheme with several parameters that can be chosen to trade off space,computation,and the pact of errors.Our hashing approach also yields a simple alternative structure with the same functionality as a counting Bloom filter that uses much less space.We show how ACSMs can be used for video congestion control.Using an ACSM,a router can implement sophisticated Active Queue Management (AQM)techniques for video traffic (without the need for standards changes to mark packets or change video formats),with a factor of four reduction in memory compared to full-state schemes and with very little error.We also show that ACSMs show promise for real-time detection of P2P traffic.
Defective protein folding as a basis of human disease (1995)

The ability of a polypeptide to fold into a unique, functional, three-dimensional structure in vivo is dependent upon its amino acid sequence and the function of molecular chaperone proteins and enzymes that catalyse folding. Intense study of the physical chemistry and cell biology of folding have greatly aided our understanding of the mechanisms normally employed. Evidence is accumulating that many disease-causing mutations and modifications exert their effects by altering protein folding. Here we discuss the pathobiology of these processes.
High resolution allelotype of microdissected primary nasopharyngeal carcinoma. (2000)

Nasopharyngeal carcinoma (NPC) is a common cancer in South China but is rare in other parts of the world. To better understand the molecular basis of this cancer, we performed high-resolution allelotyping on 27 microdissected primary tumors using 382 microsatellite markers. We have detected high frequencies of allelic imbalance on 3p (96.3%), 9p (85.2%), 9q (88.9%), 11q (74.1%), 12q (70.4%), 13q (55.6%), 14q (85.2%), and 16q (55.6%). Nonrandom allelic changes of 12q and 16q were revealed for the first time. In addition, loss of heterozygosity on chromosomal arms 1p (37.0%), 5q (44.4%), and 12p (44.4%) were also common in NPC. Multiple minimally deleted regions, 7-40 cM, were identified at 3p14-24.2, 11q21-23, 13q12-14, 13q31-32, 14q24-32, and 16q22-23. Frequent deletions of these minimally deleted regions implied the presence of tumor suppressor genes that may be involved in the development of NPC. Consistent loss of heterozygosity on 3p, 9p, and 14q in almost all tumors suggested that such changes are critical events in NPC tumorigenesis.
Orientation-Controlled Self-Assembled Nanolithography Using a Polystyrene−Polydimethylsiloxane Block Copolymer (2007)

Templated self-assembly of a cylinder-forming poly(styrene-b-dimethylsiloxane) (PS-PDMS) diblock copolymer has been investigated for nanolithography applications. The large chi-parameter of the blocks and the use of a PDMS-brush substrate surface treatment are especially advantageous for achieving long-range ordering and minimizing defect densities, and the high Si content in PDMS leaves a robust oxide etch mask after two-step reactive ion etching. By adjusting mesa width and solvent-annealing vapor pressure and time, the cylinders can be intentionally oriented parallel or perpendicular to the trench walls. Pattern transfer into thin silica is also demonstrated. This block copolymer system has excellent characteristics for self-assembled nanolithography applications.
Mass Spectrometry in Proteomics (2001)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMass Spectrometry in ProteomicsRuedi Aebersold and David R. GoodlettView Author Information The Institute for Systems Biology, 4225 Roosevelt Way NE, Seattle, Washington 98105 Cite this: Chem. Rev. 2001, 101, 2, 269–296Publication Date (Web):January 23, 2001Publication History Received24 July 2000Published online23 January 2001Published inissue 1 February 2001https://pubs.acs.org/doi/10.1021/cr990076hhttps://doi.org/10.1021/cr990076hresearch-articleACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views10989Altmetric-Citations1009LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biological databases,Ions,Monomers,Peptides and proteins,Protein identification Get e-Alerts
Making Memristive Neural Network Accelerators Reliable (2018)

Deep neural networks (DNNs) have attracted substantial interest in recent years due to their superior performance on many classification and regression tasks as compared to other supervised learning models. DNNs often require a large amount of data movement, resulting in performance and energy overheads. One promising way to address this problem is to design an accelerator based on in-situ analog computing that leverages the fundamental electrical properties of memristive circuits to perform matrix-vector multiplication. Recent work on analog neural network accelerators has shown great potential in improving both the system performance and the energy efficiency. However, detecting and correcting the errors that occur during in-memory analog computation remains largely unexplored. The same electrical properties that provide the performance and energy improvements make these systems especially susceptible to errors, which can severely hurt the accuracy of the neural network accelerators. This paper examines a new error correction scheme for analog neural network accelerators based on arithmetic codes. The proposed scheme encodes the data through multiplication by an integer, which preserves addition operations through the distributive property. Error detection and correction are performed through a modulus operation and a correction table lookup. This basic scheme is further improved by data-aware encoding to exploit the state dependence of the errors, and by knowledge of how critical each portion of the computation is to overall system accuracy. By leveraging the observation that a physical row that contains fewer 1s is less susceptible to an error, the proposed scheme increases the effective error correction capability with less than 4.5% area and less than 4.7% energy overheads. When applied to a memristive DNN accelerator performing inference on the MNIST and ILSVRC-2012 datasets, the proposed technique reduces the respective misclassification rates by 1.5x and 1.1x.
Multiple, Tandem Plasmid Integration inSaccharomyces cerevisiae (1983)

Nonreplicating plasmids transform Saccharomyces cerevisiae by recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.
Combination Therapy for Infection Due to Human Immunodeficiency Virus Type 1 (1994)

The preliminary results of the Concorde trial demonstrated the transient clinical benefit of monotherapy with zidovudine (AZT) in asymptomatic persons infected with human immunodeficiency virus type 1 (HIV-1). This result, which has been widely disseminated and discussed, was predictable given the previous demonstration of the development of resistance to AZT in isolates from individuals receiving prolonged treatment with the drug and given the finding that didanosine (ddI) is more efficacious than continued therapy with AZT in individuals who have received > or = 6 months of AZT monotherapy. On the basis of these findings, interest in combinations of antiretroviral agents has continued to grow. Many in vitro studies of nucleoside and nonnucleoside inhibitors of reverse transcriptase combined with interferon-alpha or inhibitors of protease have been published. In addition, numerous clinical trials of various combinations have been completed or are under way. Dr. Martin Hirsch and his colleagues at the Massachusetts General Hospital have been among the leaders of this effort. He and Dr. Angela Caliendo review, in this AIDS Commentary, the current state of our knowledge regarding the potential utility of combination therapy for infection with HIV-1.
Saponin and Sapogenol. IV. Seeds Sapogenols of <I>Aesculus turbinata</I> B<SUB>LUME</SUB>. On the Configuration of Hydroxyl Functions in Ring E of Aescigenin, Protoaescigenin, and Isoaescigeninin Relation to Barringtogenol C and Theasapogenol A (1971)

1) The sapogenol composition of Japanese horsechestnuts (Aesculus turbinata BLUME, Hippocastanaceae) has been clarified asbarringtogenol C (1), aescigenin (VI), protoaescigenin (VII) and 16-desoxy-barringtogenol C (IX). 2) The structure elucidation of the new sapogenol named 16-desoxy-barringtogenol C (IX) has been described in detail. 3) The full account on the configuration of hydroxyl functions in ring E of aescigenin (VI; 22α-OH), protoaescigenin (VII; 21β-0H, 22α-OH) and isoaescigenin (VIII; 21α-OH, 22α-OH) in relation to barringtogenol C (I) and theasapogenol A (II) has been presented.
Asymmetric dihydroxylation route to a dipeptide isostere of a protease inhibitor: enantioselective synthesis of the core unit of ritonavir (1999)

An enantioselective synthesis of the dipeptide isostere of ritonavir has been accomplished utilizing Sharpless asymmetric hydroxylation as the key step.
Expression of a Functionally Active gp91phox-Containing Neutrophil-Type NAD(P)H Oxidase in Smooth Muscle Cells From Human Resistance Arteries (2002)

A major source of vascular smooth muscle cell (VSMC) superoxide is NAD(P)H oxidase. However, the molecular characteristics and regulation of this enzyme are unclear. We investigated whether VSMCs from human resistance arteries (HVSMCs) possess a functionally active, angiotensin II (Ang II)-regulated NAD(P)H oxidase that contains neutrophil oxidase subunits, including p22phox, gp91phox, p40phox, p47phox, and p67phox. mRNA expression of gp91phox homologues, nox1 and nox4, was also assessed in HVSMCs, human aortic smooth muscle cells, and rat VSMCs. HVSMCs were obtained from resistance arteries from gluteal biopsies of healthy subjects. gp91phox and nox4, but not nox1, were detected in HVSMCs. Nox1 and nox4, but not gp91phox, were expressed in human aortic smooth muscle cells and rat VSMCs. All NAD(P)H oxidase subunits were present in HVSMCs as detected by reverse transcriptase-polymerase chain reaction and immunoblotting. Ang II increased NAD(P)H oxidase subunit abundance. These effects were inhibited by cycloheximide. Acute Ang II stimulation (10 to 15 minutes) increased p47phox serine phosphorylation and induced p47phox and p67phox translocation. This was associated with NAD(P)H oxidase activation. In cells transfected with gp91phox antisense oligonucleotides, Ang II-mediated actions were abrogated. NADPH-induced superoxide generation was reduced by gp91ds-tat and apocynin, inhibitors of p47phox-gp91phox interactions. Our results suggest that HVSMCs possess a functionally active gp91phox-containing neutrophil-like NAD(P)H oxidase. Ang II regulates the enzyme by inducing phosphorylation of p47phox, translocation of cytosolic subunits, and de novo protein synthesis. These novel findings provide insight into the molecular regulation of NAD(P)H oxidase by Ang II in HVSMCs. Furthermore, we identify differences in gp91phox homologue expression in VSMCs from rats and human small and large arteries.
Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability (2003)

As part of a program to discover improved glycoside hydrolase family 12 (GH 12) endoglucanases, we have studied the biochemical diversity of several GH 12 homologs. The H. schweinitzii Cel12A enzyme differs from the T. reesei Cel12A enzyme by only 14 amino acids (93% sequence identity), but is much less thermally stable. The bacterial Cel12A enzyme from S. sp. 11AG8 shares only 28% sequence identity to the T. reesei enzyme, and is much more thermally stable. Each of the 14 sequence differences from H. schweinitzii Cel12A were introduced in T. reesei Cel12A to determine the effect of these amino acid substitutions on enzyme stability. Several of the T. reesei Cel12A variants were found to have increased stability, and the differences in apparent midpoint of thermal denaturation (T(m)) ranged from a 2.5 degrees C increase to a 4.0 degrees C decrease. The least stable recruitment from H. schweinitzii Cel12A was A35S. Consequently, the A35V substitution was recruited from the more stable S. sp. 11AG8 Cel12A and this T. reesei Cel12A variant was found to have a T(m) 7.7 degrees C higher than wild type. Thus, the buried residue at position 35 was shown to be of critical importance for thermal stability in this structural family. There was a ninefold range in the specific activities of the Cel12 homologs on o-NPC. The most and least stable T. reesei Cel12A variants, A35V and A35S, respectively, were fully active. Because of their thermal tolerance, S. sp. 11AG8 Cel12A and T. reesei Cel12A variant A35V showed a continual increase in activity over the temperature range of 25 degrees C to 60 degrees C, whereas the less stable enzymes T. reesei Cel12A wild type and the destabilized A35S variant, and H. schweinitzii Cel12A showed a decrease in activity at the highest temperatures. The crystal structures of the H. schweinitzii, S. sp. 11AG8, and T. reesei A35V Cel12A enzymes have been determined and compared with the wild-type T. reesei Cel12A enzyme. All of the structures have similar Calpha traces, but provide detailed insight into the nature of the stability differences. These results are an example of the power of homolog recruitment as a method for identifying residues important for stability.
Infrared Multiphoton Dissociation in an External Ion Reservoir (1999)

In this work we present a novel scheme for performing infrared multiphoton dissociation (IRMPD) external to the mass analyzer in an external ion reservoir consisting of an rf-only multipole and a pair of electrostatic lens elements. Ions generated by electrospray ionization (ESI) are accumulated in an rf-only hexapole and dissociated by irradiation at 10.6 microns from a CW CO2 laser in the source region of the mass spectrometer. This scheme is unique from other IRMPD schemes as dissociation occurs in a spatially distinct region of the spectrometer and is independent of the mass spectrometry platform used to analyze the fragment ions. The effectiveness of the technique is demonstrated with ESI IRMPD FTICR mass spectrometry of a 20-mer phosphorothioate oligonucleotide. A comparison of the external IRMPD scheme with nozzle-skimmer dissociation and conventional in-cell IRMPD reveals a significant improvement in signal-to-noise ratio and fragment yield, particularly for larger, more highly charged fragment ions.