gebhart/patrepeval huggingface dataset:
Dynamic pricing by scalable energy management systems &#x2014; Field experiences and simulation results using PowerMatcher (2012)

Response of demand, distributed generation and electricity storage (e.g. vehicle to grid) will be crucial for power systems management in the future smart electricity grid. In this paper, we describe a smart grid technology that integrates demand and supply flexibility in the operation of the electricity system through the use of dynamic pricing. Over the last few years, this technology has been researched and developed into a market-ready system, and has been deployed in a number of successful field trials. Recent field experiences and simulation studies show the potential of the technology for network operations (e.g. congestion management and black-start support), for market operations (e.g. virtual power plant operations), and integration of large-scale wind power generation. The scalability of the technology, i.e. the ability to perform well under mass-application circumstances, has been proved in a targeted field experiment. This paper gives an overview of the results of two field trials and three simulation studies. In these trials and simulations, demand and supply response from real and simulated electrical vehicles, household appliances and heating systems (heat pumps and micro co-generation) has been successfully coordinated to reach specific smart grid goals.
Blood-brain barrier dysfunction in disorders of the developing brain (2015)

Disorders of the developing brain represent a major health problem. The neurological manifestations of brain lesions can range from severe clinical deficits to more subtle neurological signs or behavioral problems and learning disabilities, which often become evident many years after the initial damage. These long-term sequelae are due at least in part to central nervous system immaturity at the time of the insult. The blood-brain barrier (BBB) protects the brain and maintains homeostasis. BBB alterations are observed during both acute and chronic brain insults. After an insult, excitatory amino acid neurotransmitters are released, causing reactive oxygen species (ROS)-dependent changes in BBB permeability that allow immune cells to enter and stimulate an inflammatory response. The cytokines, chemokines and other molecules released as well as peripheral and local immune cells can activate an inflammatory cascade in the brain, leading to secondary neurodegeneration that can continue for months or even years and finally contribute to post-insult neuronal deficits. The role of the BBB in perinatal disorders is poorly understood. The inflammatory response, which can be either acute (e.g., perinatal stroke, traumatic brain injury) or chronic (e.g., perinatal infectious diseases) actively modulates the pathophysiological processes underlying brain injury. We present an overview of current knowledge about BBB dysfunction in the developing brain during acute and chronic insults, along with clinical and experimental data.
Antisense inhibition of protein phosphatase 2C accelerates cold acclimation in Arabidopsis thaliana (2001)

Summary Two related protein phosphatases 2C, ABI1 and AtPP2CA have been implicated as negative regulators of ABA signalling. In this study we characterized the role of AtPP2CA in cold acclimation. The pattern of expression of AtPP2CA and ABI1 was studied in different tissues and in response to abiotic stresses. The expression of both AtPP2CA and ABI1 was induced by low temperature, drought, high salt and ABA. The cold and drought‐induced expression of these genes was ABA‐dependent, but divergent in various ABA signalling mutants. In addition, the two PP2C genes exhibited differences in their tissue‐specific expression as well as in temporal induction in response to low temperature. To elucidate the function of AtPP2CA in cold acclimation further, the corresponding gene was silenced by antisense inhibition. Transgenic antisense plants exhibited clearly accelerated development of freezing tolerance. Both exposure to low temperature and application of ABA resulted in enhanced freezing tolerance in antisense plants. These plants displayed increased sensitivity to ABA both during development of frost tolerance and during seed germination, but not in their drought responses. Furthermore, the expression of cold‐and ABA‐induced genes was enhanced in transgenic antisense plants. Our results suggest that AtPP2CA is a negative regulator of ABA responses during cold acclimation.
Effects of Preventing Periparturient Hypocalcemia in Cows by Parathyroid Hormone Administration on Hematology, Conglutinin, Immunoglobulin, and Shedding of Staphylococcus aureus in Milk (1990)

The effects of hypocalcemia at parturition on concentrations of serum immunoglobulin and conglutinin, number of bacteria shed into milk, and leukograms of dairy cows were investigated from -4 wk prepartum to 4 wk postpartum. Ten healthy multiparous Holstein cows were fed a high calcium diet to induce hypocalcemia at parturition. Five cows received intramuscular parathyroid hormone to prevent hypocalcemia at parturition. All cows experienced a leukopenia (attributable to an absolute and relative neutropenia) during the 1st wk after calving, decreased serum conglutinin activity during the first 3 wk postpartum, and decreased concentration of serum IgG1 during the 3 wk before calving. At parturition, a large increase in organisms was found in foremilk (1000 to 10,000 times more than prepartum values). Neither the hematological changes nor the decreased immunoglobulin concentration was influenced by hypocalcemia or the development of milk fever. This implies that the degree of hypocalcemia observed did not have a large or irreversible influence on bacterial infection, hematological, or humoral immunity changes in periparturient cows.
Modulation of Calcium Oxalate Crystallization by Linear Aspartic Acid-Rich Peptides (2006)

Calcium oxalate monohydrate (COM) kidney stone formation is prevented in most humans by urinary crystallization inhibitors. Urinary osteopontin (OPN) is a prototype of the aspartic acid-rich proteins (AARP) that modulate biomineralization. Synthetic poly(aspartic acids) that resemble functional domains of AARPs provide surrogate molecules for exploring the role of AARPs in biomineralization. Effects of linear aspartic acid-rich peptides on COM growth kinetics and morphology were evaluated by the combination of constant composition (CC) analysis and atomic force microscopy (AFM). A spacer amino acid (either glycine or serine) was incorporated during synthesis after each group of 3 aspartic acids (DDD) in the 27-mer peptide sequences. Kinetic CC studies revealed that the DDD peptide with serine spacers (DDDS) was more than 30 times more effective in inhibiting COM crystal growth than the DDD peptide with glycine spacers (DDDG). AFM revealed changes in morphology on (010) and (-101) COM faces that were generally similar to those previously described for OPN and citrate, respectively. At comparable peptide levels, the effects of step pinning and reduced growth rate caused by DDDS were remarkably greater. In CC nucleation studies, DDDS caused a greater prolongation of induction periods than DDDG. Thus, nucleation studies link changes in interfacial energy caused by peptide adsorption to COM to the CC growth and AFM results. These studies indicate that, in addition to the number of acidic residues, the contributions of other amino acids to the conformation of DDD peptides are also important determinants of the inhibition of COM nucleation and growth.
Influence of Intestinal Segment and Configuration on the Outcome of Augmentation Enterocystoplasty (1986)

The clinical and urodynamic results of enterocystoplasty were compared in 3 groups of patients in which the intact ileocecal segment (10 patients), tubular sigmoid segment (16) or sigmoid cup-patch segment (8) was used. In all 3 groups the upper urinary tract and renal function improved or remained stable, and the functional bladder capacity increased. All patients experienced involuntary, volume-dependent vesical contractions of comparable intensity 3 months postoperatively. After a mean followup of 19.4 +/- 8.9 months only 12 per cent of the patients with a cup-patch configuration demonstrated volume-dependent contractions, compared to all patients with ileocecal cystoplasty and 94 per cent with sigmoid tubular cystoplasty. The intensity of these contractions was significantly less in the cup-patch configuration group compared to the other groups. Patients with cup-patch cystoplasty achieved a significantly higher rate of urinary continence than those in the other 2 groups. It appears that the sigmoid cup-patch configuration may provide a lower pressure system and better continence compared to the intact ileocecal or tubular sigmoid segment, although the latter techniques have specific applications when confronted with a wide gap between the ureters and bladder or when dilated ureters require anastomosis to the bowel.
Computing the Lines Piercing Four Lines (1992)

Given four distinct lines in R^3 there exist zero, one, two, or various infinities of lines incident on the given lines. We wish to characterize and compute the set of incident lines in a numerically stable way. We use the Plucker coordinatization of lines to cast this problem as a null-space computation in R^5, and show how the singular value decomposition (SVD) yields a simple, stable characterization of the incident lines. Finally, we enumerate the types of input degeneracies that may arise, and describe the solution set of lines in each case
A New Strategy for Improved Nicotine Vaccines Using Conformationally Constrained Haptens (2003)

Constrained nicotine analogues were synthesized and coupled to the carrier protein KLH. The immunogenic effects were compared to those using our previously designed flexible nicotine hapten. Immunization of mice with the constrained hapten conjugates resulted in highly increased antibody titers and affinity for nicotine.
J.M. Coetzee, R.G. Howarth, south to south (2014)

This article was part of the 2014 special issue of New Scholar entitled 'Australian Literature: The Road Ahead'. The issue followed the 2013 Macquarie University conference of the same name in seeking to examine trends, manifestations and solidifications in Australian writing and modes of analysis which reveal new research frontiers.
Novel Near-Infrared Cyanine Fluorochromes:  Synthesis, Properties, and Bioconjugation (2002)

Recently, near-infrared (NIR) fluorescence light has been applied to image various biological events in vivo, because it penetrates tissue more efficiently than light in the visible spectrum. Compounds exhibiting fluorescent properties in the NIR range are key elements for this upcoming optical imaging technology. In this paper, we report the synthesis of four new, water-soluble NIR cyanine fluorochromes which have superior chemical stability and optical properties. Each fluorochrome was designed with a monoreactive carboxyl group for labeling purposes. When multiple fluorochromes were attached to a single macromolecule, fluorescence quenching was observed. On the basis of this property, a novel autoquenched enzyme sensitive NIR fluorescence probe was prepared.
Transcutaneous Immunization with Bacterial ADP-Ribosylating Exotoxins as Antigens and Adjuvants (1999)

Transcutaneous immunization (TCI) is a new technique that uses the application of vaccine antigens in a solution on the skin to induce potent antibody responses without systemic or local toxicity. We have previously shown that cholera toxin (CT), a potent adjuvant for oral and nasal immunization, can induce both serum and mucosal immunoglobulin G (IgG) and IgA and protect against toxin-mediated mucosal disease when administered by the transcutaneous route. Additionally, CT acts as an adjuvant for coadministered antigens such as tetanus and diphtheria toxoids when applied to the skin. CT, a member of the bacterial ADP-ribosylating exotoxin (bARE) family, is most potent as an adjuvant when the A-B subunits are present and functional. We now show that TCI induces secondary antibody responses to coadministered antigens as well as to CT in response to boosting immunizations. IgG antibodies to coadministered antigens were also found in the stools and lung washes of immunized mice, suggesting that TCI may target mucosal pathogens. Mice immunized by the transcutaneous route with tetanus fragment C and CT developed anti-tetanus toxoid antibodies and were protected against systemic tetanus toxin challenge. We also show that bAREs, similarly organized as A-B subunits, as well as the B subunit of CT alone, induced antibody responses to themselves when given via TCI. Thus, TCI appears to induce potent, protective immune responses to both systemic and mucosal challenge and offers significant potential practical advantages for vaccine delivery.
Tolerization of dendritic cells by HLA‐G (2005)

The expression of HLA-G at the fetal-maternal interface during pregnancy and in transplanted tissue makes this a key molecule in the acceptance of a semiallogeneic fetus and allogeneic transplant. Dendritic cells (DC) play a critical role in the control of innate and adaptive immune responses. DC are present in maternal decidua, but must be kept under tight control. Here we describe the mechanism of tolerization of DC by HLA-G through inhibitory receptor interactions. The HLA-G-ILT (immunoglobulin-like transcript) interaction leads to development of tolerogenic DC with the induction of anergic and immunosuppressive T cells. Using human monocyte-derived DC and ILT4-transgenic mice, we show that (i) HLA-G induces the development of tolerogenic DC with arrest maturation/activation of myeloid DC, (ii) HLA-G-modified DC induce differentiation of anergic and immunosuppressive CD4(+) and CD8(+) effector T cells, and (iii) the gene expression profile provides evidence that HLA-G induces tolerogenic DC by disruption of the MHC class II presentation pathway. Ligation of ILT4 receptor on DC from transgenic mice diminished peptide presentation by MHC class II molecules and significantly prolonged allograft survival. These findings provide support that HLA-G is an important tolerogenic molecule on DC for the acceptance of a semiallogeneic fetus and transplanted tissue/organ.
Combined Dendritic Cell Cryotherapy of Tumor Induces Systemic Antimetastatic Immunity (2005)

These results present cryoimmunotherapy as a novel approach for systemic treatment of cancer. We envisage that cryotherapy of tumors combined with subsequent in situ immunotherapy by autologous unmodified immature dendritic cells can be applied in practice.
Nitrogen Fixation by Members of the Tribe Klebsielleae (1965)

Mahl, M. C. (University of Wisconsin, Madison), P. W. Wilson, M. A. Fife, and W. H. Ewing. Nitrogen fixation by members of the tribe Klebsielleae. J. Bacteriol. 89:1482-1487. 1965.-Strains of species of the tribe Klebsielleae were tested for nitrogen fixation by inoculating actively growing cultures into side-arm flasks containing a medium with a growth-limiting quantity of combined nitrogen (25 mug of nitrogen per ml as ammonium sulfate). The flasks were evacuated, filled with pure N(2), sealed, and placed on a shaker at 30 C. Growth was followed by optical-density measurements; maximal growth was obtained in 9 to 10 hr. Yeast extract was then added as a source of amino acids to shorten the induction time for the nitrogen-fixing enzymes. Fixation was determined either by estimating total nitrogen with a semimicro Kjeldahl technique or by exposing 20- to 22-hr-old samples to an atmosphere of N(2) (15) and helium for 5 hr and then analyzing the digested sample for N(15) in a mass spectrometer. None of the 22 strains of the two Enterobacter (formerly Aerobacter) species fixed nitrogen; neither did any of eight strains of Serratia species that were tested. Of 31 strains of Klebsiella pneumoniae, 13 incorporated atmospheric nitrogen. Net nitrogen fixed ranged from 17 to 65 mug/ml. It is concluded that these facultative anaerobic, gram-negative, nitrogen-fixing rods should be placed in the genus Klebsiella pneumoniae. The nitrogen-fixing organism tentatively classified as Achromobacter N-4 should also be changed to K. pneumoniae strain N-4, because it has been found to be an anaerogenic strain of K. pneumoniae.
Ionic Liquid as a Green Solvent for Lignin (2007)

Abstract This study examined the application of select ionic liquids (ILs) as aprotic green solvents for lignin. Dissolution experiments were carried out employing lignin isolated from pine kraft pulp. Up to 20 wt% lignin could be dissolved in [hmim][CF3SO3], [mmim][MeSO4] and [bmim][MeSO4]. For the [bmim]+‐containing ionic liquids, the order of lignin solubility for varying anions was: [MeSO4]−>Cl−∼Br−⋙[PF6]−, indicating that the solubility of lignin was principally influenced by the nature of the anions. Ionic liquids containing large, non‐coordinating anions [PF4]− and [PF6]− were unsuitable as a solvent for lignin. 13C nuclear magnetic resonance (NMR) analyses of lignin and model compounds showed that 13C signals using ionic liquid as a solvent were shifted up‐field by δ 0.1 to 1.9 ppm in comparison to 13C NMR data acquired using dimethyl sulfoxide (DMSO) as the solvent.
Accumulation of genetic defects during astrocytoma progression (1992)

These results identify a genetic approach to defining degrees of tumor progression and a means for determining the genomic locations of genes involved in the pathway as a prelude to their molecular isolation and characterization. They provide a molecular genetic-based oncology with clinical utility in differential pathologic findings, in disease groupings for therapeutic purposes, and in prenatal identification of latent disease carriers.
Fluorometric assessment of Gram-negative bacterial permeabilization (2000)

Uptake of the fluorescent probe 1-N-phenylnaphthylamine (NPN), as adapted to an automated spectrofluorometer enabling multiwell reading of microtitre plates, was applied to determine permeability changes in Gram-negative bacteria. An intact outer membrane is a permeability barrier, and excludes hydrophobic substances such as NPN but, once damaged, it can allow the entry of NPN to the phospholipid layer, resulting in prominent fluorescence. With Escherichia coli O157, Pseudomonas aeruginosa, and Salmonella typhimurium as test organisms and ethylenediaminetetraacetic acid and sodium hexametaphosphate as the model permeabilizers, quantitative and highly reproducible NPN uptake levels were obtained that differed characteristically between the test bacteria. Furthermore, citric acid was shown to be a potent permeabilizer at millimolar concentrations, its effect being partly (Ps. aeruginosa, Salm. typhimurium) or almost totally (E. coli O157) abolished by MgCl2, suggesting that part of the action occurs by chelation. Sodium citrate induced weak NPN uptake, which was totally abolished by MgCl2. In conclusion, the NPN uptake assay with the automated spectrofluorometer serves as a convenient method in analysing and quantifying the effects of external agents, including potential food preservatives, on Gram-negative bacteria.
Redox Behavior of Phenyl-Terpyridine-Substituted Artificial Oligopeptides Cross-Linked by Co and Fe (2007)

This work presents the synthesis and characterization of metalated oligopeptide duplex assemblies composed of artificial oligopeptides bearing tethered phenyl terpyridine ligands that are coordinated to Co(II) and Fe(II) ions. The metals cross-link the oligopeptide strands, forming duplex structures. UV-vis spectrophotometric titrations that monitor the absorbance of the metal complexes' characteristic MLCT bands demonstrate stoichiometric metal chelation. Anodic peaks in the cyclic voltammograms of these molecules are consistent with one-electron oxidative reactions without strong coupling between the metal complexes. In separate chronocoulometry measurements, the diffusion coefficients of the metal complexes decrease with increasing oligopeptide length, suggesting the primary products are metal-linked oligopeptide duplex assemblies. Larger metalated oligopeptides adsorb to electrode surfaces during cyclic voltammetry and yield irreversibly adsorbed electroactive films with thicknesses that depend on the number of voltage cycles. Electrochemical and spectroelectrochemical investigations of the films on Pt and ITO electrodes show that the electron transfers in the adsorbed films are chemically reversible but are kinetically quasi-reversible.
The attenuation of learning impairments induced after exposure to CO or trimethyltin in mice by sigma (σ) receptor ligands involves both σ1 and σ2 sites (1999)

1. Sigma (sigma) receptor ligands were previously reported to alleviate learning and memory impairments on several pharmacological and pathological rodent models of amnesia. Such effect was demonstrated as involving the sigma1 subtype of sigma receptor. 2. In this study, we characterized the pharmacological effect mediated by sigma ligands on two lesional models of amnesia in mice: (1) the hypoxia-related learning and memory impairment model induced by repeated exposure to carbon monoxide (CO) gas; and (2) the intoxication with trimethyltin (1 mg kg(-1)). 3. The selective sigma1 ligand PRE-084 (1 mg kg(-1)) or the non-selective sigma1/sigma2 compounds DTG (0.1 mg kg(-1)), BD1008 (3 mg kg(-1)), and haloperidol (0.1 mg kg(-1)) reversed significantly the spontaneous alternation deficits observed 7 days after exposure to CO or 14 days after intoxication with trimethyltin. 4. The selective sigma1 receptor antagonist NE-100 (1 mg kg(-1)) was ineffective by itself, but blocked completely the PRE-084 effects, partially the DTG effects, and did not affect the effects induced by BD1008 or haloperidol. 5. A similar pharmacological profile was observed in the step-down type passive avoidance test performed 8 days after exposure to CO. 6. These results show that, in contrast to the previously reported amnesia models, the impairments induced after exposure to CO or intoxication with trimethyltin could be alleviated not only by sigma1 receptor agonists but also by sigma2 agonists. The particular pattern of neurodegeneration observed in these lesional models may explain these differences.
Similarity searching in databases of three-dimensional molecules and macromolecules (1992)

This paper discusses algorithmic techniques for measuring the degree of similarity between pairs of three-dimensional (3-D) chemical molecules represented by interatomic distance matrices. A comparison of four methods for the calculation of 3-D structural similarity suggests that the most effective one is a procedure that identifies pairs of atoms, one from each of the molecules that are being compared, that lie at the center of geometrically-related volumes of 3-D space. This atom mapping method enables the calculation of a wide range of types of intermolecular similarity coefficient, including measures that are based on physicochemical data. Massively-parallel implementations of the method are discussed, using the AMT Distributed Array Processor, that achieve a substantial increase in performance when compared with a sequential implementation on a UNIX workstation. Current work involves the use of angular information and the extension of the method to field-based similarity searching. Similarity searching in 3-D macromolecules is effected by the use of a maximal common subgraph (MCS) isomorphism algorithm with a novel, graph-based representation of the tertiary structures of proteins. This algorithm is being used to identify similarities between the 3-D structures of proteins in the Brookhaven Protein Data Bank; its use is exemplified by searches involving the NAD-binding fold motif.