gebhart/patrepeval huggingface dataset:
Self-Assembled Ternary Complex of Cationic Dendrimer, Cucurbituril, and DNA:  Noncovalent Strategy in Developing a Gene Delivery Carrier (2002)

A ternary complex of PPI-DAB dendrimer [(1,4-diaminobutane); Gen = N; dendri-poly(propyleneimine); -[NHC(=O)CH(2)NH(2)(+)(CH(2))(4)NH(3)(+)](z)()], DNA, and cucurbituril (CB) was evaluated as an example of a totally self-assembled gene delivery carrier. The complex was formed in a noncovalent way in which DNA interacts with PPI-DAB electrostatistically and CB with PPI-DAB through multiple noncovalent interactions. Dynamic light scattering data indicated that the diameter and size distributions of the complexes were dependent upon the sequence of mixing of each component with unimodal distribution ranging from 150.8 to 210.2 nm under favorable conditions. Fluorescence studies showed the quantitative binding of CB to PPI-DAB after ternary complex formation. The complex was able to transfect mammalian cells with high efficiency and the cytotoxicity of the PPI-DAB/CB complex was relatively low.
The Burroughs Scientific Processor (BSP) (1982)

The Burroughs Scientific Processor (BSP), a high-performance computer system, performed the Department of Energy LLL loops at roughly the speed of the CRAY-1. The BSP combined parallelism and pipelining, performing memory-to-memory operations. Seventeen memory units and two crossbar switch data alignment networks provided conflict-free access to most indexed arrays. Fast linear recurrence algorithms provided good performance on constructs that some machines execute serially. A system manager computer ran the operating system and a vectorizing Fortran compiler. An MOS file memory system served as a high bandwidth secondary memory.
Mitochondrial Biogenesis in Mammals: The Role of Endogenous Nitric Oxide (2003)

Nitric oxide was found to trigger mitochondrial biogenesis in cells as diverse as brown adipocytes and 3T3-L1, U937, and HeLa cells. This effect of nitric oxide was dependent on guanosine 3',5'-monophosphate (cGMP) and was mediated by the induction of peroxisome proliferator-activated receptor gamma coactivator 1alpha, a master regulator of mitochondrial biogenesis. Moreover, the mitochondrial biogenesis induced by exposure to cold was markedly reduced in brown adipose tissue of endothelial nitric oxide synthase null-mutant (eNOS-/-) mice, which had a reduced metabolic rate and accelerated weight gain as compared to wild-type mice. Thus, a nitric oxide-cGMP-dependent pathway controls mitochondrial biogenesis and body energy balance.
Modelling voter behaviours by geographic information technology: a case of Hong Kong in 2004 (2010)

Maps have been used widely to communicate election results and voter turnout. This article presents a practical approach to extracting and assembling the profiles of Hong Kong voters in the 2004 Legislative Council Election. It describes the use of modelling functions in a geographic information system to augment the examination of demographic and socio-economic constructs of voters and their voting behaviours. Voter profiles were used as predictors in forecasting election results. The predictive power of our model was assessed through visual and qualitative comparison with actual results of the election. The comparison showed that both maps exhibited similar patterns. © 2010 Taylor &amp; Francis.
Increasing the Potency and Breadth of an HIV Antibody by Using Structure-Based Rational Design (2011)

Antibodies against the CD4 binding site (CD4bs) on the HIV-1 spike protein gp120 can show exceptional potency and breadth. We determined structures of NIH45-46, a more potent clonal variant of VRC01, alone and bound to gp120. Comparisons with VRC01-gp120 revealed that a four-residue insertion in heavy chain complementarity-determining region 3 (CDRH3) contributed to increased interaction between NIH45-46 and the gp120 inner domain, which correlated with enhanced neutralization. We used structure-based design to create NIH45-46(G54W), a single substitution in CDRH2 that increases contact with the gp120 bridging sheet and improves breadth and potency, critical properties for potential clinical use, by an order of magnitude. Together with the NIH45-46-gp120 structure, these results indicate that gp120 inner domain and bridging sheet residues should be included in immunogens to elicit CD4bs antibodies.
Pre- and Posteruptive Effects of Low Doses of Strontium on Dental Caries in the Rat (1980)

Concentrations of strontium (Sr) were given preeruptively to rats at 0.0, 0.5, 1.0, 2.5, 5.0, and 7.5 μg Sr/10 g body weight by esophageal intubation. At weaning, the same rats were given 0, 5, 10, 25, 50, and 75 mg Sr/liter via the drinking water. Caries scores were recorded for buccolingual, morsal, sulcal, and proximal lesions in all rats. Reductions in caries scores were only seen for buccolingual scores. Maximum reductions in buccolingual scores were found in rats given 7.5 μg Sr/10 g body weight preeruptively and 75 mg Sr/liter posteruptively. Uptake of strontium by enamel was significantly correlated with drinking water concentrations.
High density 3D printed microfluidic valves, pumps, and multiplexers (2016)

We demonstrate that a custom resin with the right optical properties enables a digital light processor stereolithographic (DLP-SLA) 3D printer to fabricate microfluidic devices with densely integrated active elements in a 3D layout.
Business Summary New York : 1986 (1987)

A.E. Res. 85-15
Bill Clinton's "Three Noes" and Taiwan's Future (1999)

Diplomatic historians and political scientists have warned that when great powers make war or love, the smaller countries nearby feel the tremors.1 As they look back, President Bill Clinton's 1998 summit visit to China marked the emergence of a new world order: With the end of the Cold War, China is poised to become a potential superpower, and its future evolution will have great implications for the U.S. "For better or worse, the U.S.Chinese relationship seems destined to be one of the principal pivots in international relations well into the 21st century," as Walter Russell Mead asserts.2 That much is clear, but what is not clear is how to deal with this rising power.
The p2 domain of human immunodeficiency virus type 1 Gag regulates sequential proteolytic processing and is required to produce fully infectious virions (1994)

The proteolytic processing sites of the human immunodeficiency virus type 1 (HIV-1) Gag precursor are cleaved in a sequential manner by the viral protease. We investigated the factors that regulate sequential processing. When full-length Gag protein was digested with recombinant HIV-1 protease in vitro, four of the five major processing sites in Gag were cleaved at rates that differ by as much as 400-fold. Three of these four processing sites were cleaved independently of the others. The CA/p2 site, however, was cleaved approximately 20-fold faster when the adjacent downstream p2/NC site was blocked from cleavage or when the p2 domain of Gag was deleted. These results suggest that the presence of a C-terminal p2 tail on processing intermediates slows cleavage at the upstream CA/p2 site. We also found that lower pH selectively accelerated cleavage of the CA/p2 processing site in the full-length precursor and as a peptide primarily by a sequence-based mechanism rather than by a change in protein conformation. Deletion of the p2 domain of Gag results in released virions that are less infectious despite the presence of the processed final products of Gag. These findings suggest that the p2 domain of HIV-1 Gag regulates the rate of cleavage at the CA/p2 processing site during sequential processing in vitro and in infected cells and that p2 may function in the proper assembly of virions.
Phenotype and function of human T lymphocyte subsets: Consensus and issues (2008)

In recent years, a tremendous effort has been devoted to the detailed characterization of the phenotype and function of distinct T cell subpopulations in humans, as well as to their pathway(s) of differentiation and role in immune responses. But these studies seem to have generated more questions than definitive answers. To clarify issues related to the function and differentiation of T cell subsets, one session of the MASIR 2008 conference was dedicated to this topic. Several points of consensus and discord were highlighted in the work presented during this session. We provide here an account of these points, including the relative heterogeneity of T cell subpopulations during infections with distinct pathogens, the relationship between phenotypic and functional T cell attributes, and the pathway(s) of T cell differentiation. Finally, we discuss the problems which still limit general agreement.
Chronic hypertension produced by infusion of endothelin in rats. (1990)

Endothelin, a potent vasoconstrictor peptide synthesized by the vascular smooth muscle endothelium, was chronically infused into male Sprague-Dawley rats to determine whether a long-term increase in circulating endothelin levels would cause a sustained elevation in mean arterial pressure. Rats were catheterized, housed in metabolic cages, and maintained on a fixed 6 meq/day sodium intake throughout the experiment with daily measurements including mean arterial pressure, heart rate, water intake, urine output, urinary sodium excretion, urinary potassium excretion, cardiac output, total peripheral resistance, and stroke volume. Infusion of endothelin-1 (ET-1) at rates of 3, 5, or 7.5 pmol/kg/min for 7 days was associated with significant, sustained, and dose-dependent increases in mean arterial pressure and smaller less consistent elevations in total peripheral resistance. Other parameters were unaffected. Similar results were observed in rats receiving endothelin-3 (ET-3), except that a higher dose of ET-3 was required. These results indicate that elevated blood levels of endothelin could produce a maintained hypertension without sodium or water retention and that the hemodynamic basis for the increased mean arterial pressure is similar to that seen in most other forms of experimental and clinical hypertension.
Peptidoglycan and Lipoteichoic Acid Modify Monocyte Phenotype in Human Whole Blood (2001)

ABSTRACT We examined the influence of the gram-positive cell wall products peptidoglycan (PepG) and lipoteichoic acid (LTA), compared to lipopolysaccharide (LPS), on the monocyte expression of receptors involved in antigen presentation (HLA-DR, B7.1, and B7.2), cell adhesion (intercellular adhesion molecule-1 [ICAM-1] and lymphocyte function associated antigen-3 [LFA-3]), phagocytosis (FcγRI), and cell activation (CD14). We also evaluated possible influences of the immunosuppressive drugs cyclosporine A, tacrolimus, and sirolimus on the expression of these receptors. Pretreatment of whole blood for 4 h with the immunosuppressive drugs did not influence the expression of the surface receptors in normal or stimulated blood. Stimulation with both PepG and LTA caused significant up-regulation of the surface expression of ICAM-1 and HLA-DR on whole blood monocytes, similar to that obtained with LPS, whereas B7.1, B7.2, LFA-3, and FcγRI were not modulated. PepG and LTA also caused increased expression of CD14, whereas LPS down-regulated this molecule. In contrast, we did not detect any significant influence of any of the bacterial products on the plasma concentration of soluble CD14. We hypothesized that the increased expression of surface CD14 in blood stimulated with PepG would prime for cellular activation by LPS. Indeed, we show that PepG and the partial PepG structure muramyl dipeptide acted in synergy with LPS to cause the release of tumor necrosis factor-α. The results suggest that PepG and LPS provoke partly different responses on monocyte phenotype and that CD14 may play different roles in the innate response to gram-positive and gram-negative bacteria.
Interplay between lipids and viral glycoproteins during hemolysis and fusion by influenza virus. (1986)

Since mixtures of lipids alone are known to elicit membrane fusion without participation of fusion proteins, the role of viral lipids in the so-called virus-induced hemolysis and cell fusion has been investigated, using as a model the fowl plague virus (influenza A/FPV/Rostock/H7N1). The experiments were planned in a way that allowed quantitative modification of viral lipids without changing envelope glycoproteins. Under the conditions employed, cholesterol oxidase of Nocardia erythropolis and phospholipase C of Bacillus cereus were shown to completely modify their substrates in the virus without altering virus-associated hemagglutinating and neuraminidase activities. It was found with such enzyme treatment that virus-induced hemolysis and cell fusion are greatly influenced by cholesterol and phospholipids of the envelope. It became clear, that hemolysis and fusion are differently dependent on the nature of lipid components even though mediated by the same viral glycoproteins.
An extrusion system for the processing of microcellular polymer sheets: Shaping and cell growth control (1996)

Abstract A new processing system for the extrusion of microcellular polymer sheets is presented. Specifically, the detailed design of a shaping and cell growth control system is discussed in the context of an overall extrusion system design with particular emphasis on the system level functional requirements of cell nucleation, cell growth, and shaping. The principle of the basic extrusion system design is to shape a nucleated polymer/gas solution flow under pressure and accurate temperature control. In this way, the initial cell growth is controlled so as to prevent degradation of the nucleated cell density during shaping. Two foaming die designs for satisfying the initial shaping and cell growth requirements are presented. Critical experiments are then presented which verified the concept of shaping a nucleated polymer/gas solution. Moreover, these experiments demonstrated the feasibility of the overall microcellular polymer sheet extrusion system design.
Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques. (1985)

We constructed a plasmid coexpression vector that directs the insertion of a foreign gene of interest together with the Escherichia coli beta-galactosidase (beta gal) gene into the thymidine kinase (TK) locus of the vaccinia virus genome. Tissue culture cells that had been infected with vaccinia virus were transfected with a plasmid vector containing a foreign gene. TK- recombinants could be selected by a plaque assay on TK- cells in the presence of 5-bromodeoxyuridine and distinguished from spontaneous TK- mutants by the addition of a beta-gal indicator to the agarose overlay. Plaques that expressed beta-gal stained dark blue within several hours at 37 degrees C. Alternatively, TK- selection could be eliminated, and recombinant plaques could be readily identified solely by their blue color. The reverse procedure, in which the starting virus expresses beta-gal (i.e., forms blue plaques) and the desired recombinant has deleted the entire beta-gal gene (i.e., forms white plaques), is another alternative. Each protocol was tested by constructing vaccinia virus recombinants that express hepatitis B virus surface antigen.
A Conserved Histidine Is Essential for Glycerolipid Acyltransferase Catalysis (1998)

Sequence analysis of membrane-bound glycerolipid acyltransferases revealed that proteins from the bacterial, plant, and animal kingdoms share a highly conserved domain containing invariant histidine and aspartic acid residues separated by four less conserved residues in an HX4D configuration. We investigated the role of the invariant histidine residue in acyltransferase catalysis by site-directed mutagenesis of two representative members of this family, the sn-glycerol-3-phosphate acyltransferase (PlsB) and the bifunctional 2-acyl-glycerophosphoethanolamine acyltransferase/acyl-acyl carrier protein synthetase (Aas) of Escherichia coli. Both the PlsB[H306A] and Aas[H36A] mutants lacked acyltransferase activity. However, the Aas[H36A] mutant retained significant acyl-acyl carrier protein synthetase activity, illustrating that the lack of acyltransferase activity was specifically associated with the H36A substitution. The invariant aspartic acid residue in the HX4D pattern was also important. The substitution of aspartic acid 311 with glutamic acid in PlsB resulted in an enzyme with significantly reduced catalytic activity. Substitution of an alanine at this position eliminated acyltransferase activity; however, the PlsB[D311A] mutant protein did not assemble into the membrane, indicating that aspartic acid 311 is also important for the proper folding and membrane insertion of the acyltransferases. These data are consistent with a mechanism for glycerolipid acyltransferase catalysis where the invariant histidine functions as a general base to deprotonate the hydroxyl moiety of the acyl acceptor.
Reconstruction of sequential cardiac in-plane displacement patterns on the chest wall by laser speckle interferometry (1991)

Time-average speckle interferometry has been applied to obtain displacement patterns on the chest wall produced by cardiac action, in the absence of breathing, during various phases of the cardiac cycle. This has been achieved by an electronic shutter, controlled by the electrocardiogram of the subject. The recorded holographic plates processed under identical conditions are scanned by the pointwise method to obtain the absolute displacements at various locations corresponding to the activities of the various cardiac chambers and values. These data are transformed to a 40 x 30 matrix by an interpolation method and, from this, three-dimensional displacement plots are reconstructed by an IBM PC/AT computer. These patterns shown the displacements over the entire cardiac area corresponding to the activities of various regions during the cardiac cycle. The apex and aortic valve areas show the maximum displacements during the systolic phase. During the diastolic phase the activities over the low pressure atrial regions are also observed. The results obtained outline the functional details of the normal heart and the activities over various areas are in agreement with that of other noncontact techniques.
Mechanistic Insight into the TH1-Biased Immune Response to Recombinant Subunit Vaccines Delivered by Probiotic Bacteria-Derived Outer Membrane Vesicles (2014)

Recombinant subunit vaccine engineering increasingly focuses on the development of more effective delivery platforms. However, current recombinant vaccines fail to sufficiently stimulate protective adaptive immunity against a wide range of pathogens while remaining a cost effective solution to global health challenges. Taking an unorthodox approach to this fundamental immunological challenge, we isolated the TLR-targeting capability of the probiotic E. coli Nissle 1917 bacteria (EcN) by engineering bionanoparticlate antigen carriers derived from EcN outer membrane vesicles (OMVs). Exogenous model antigens expressed by these modified bacteria as protein fusions with the bacterial enterotoxin ClyA resulted in their display on the surface of the carrier OMVs. Vaccination with the engineered EcN OMVs in a BALB/c mouse model, and subsequent mechanism of action analysis, established the EcN OMV's ability to induce self-adjuvanted robust and protective humoral and T(H)1-biased cellular immunity to model antigens. This finding appears to be strain-dependent, as OMV antigen carriers similarly engineered from a standard K12 E. coli strain derivative failed to generate a comparably robust antigen-specific TH1 bias. The results demonstrate that unlike traditional subunit vaccines, these biomolecularly engineered "pathogen-like particles" derived from traditionally overlooked, naturally potent immunomodulators have the potential to effectively couple recombinant antigens with meaningful immunity in a broadly applicable fashion.
MTN-001: Randomized Pharmacokinetic Cross-Over Study Comparing Tenofovir Vaginal Gel and Oral Tablets in Vaginal Tissue and Other Compartments (2013)

Background: Oral and vaginal preparations of tenofovir as pre-exposure prophylaxis (PrEP) for human immunodeficiency virus (HIV) infection have demonstrated variable efficacy in men and women prompting assessment of variation in drug concentration as an explanation. Knowledge of tenofovir concentration and its active form, tenofovir diphosphate, at the putative vaginal and rectal site of action and its relationship to concentrations at multiple other anatomic locations may provide key information for both interpreting PrEP study outcomes and planning future PrEP drug development.
Auditory Apparatus of the Culex Mosquito (1855)

JOURNAL ARTICLE| 01 April 1855 Original Communications: Auditory Apparatus of the Culex Mosquito CHRISTOPHER JOHNSTON CHRISTOPHER JOHNSTON Baltimore, United States Search for other works by this author on: This site PubMed Google Scholar Author and article information CHRISTOPHER JOHNSTON Baltimore, United States Online Issn: 1477-9137 Print Issn: 0021-9533 Copyright © 1855 by the Company of Biologists Ltd.1855 J Cell Sci (1855) s1-3 (10): 97–102. https://doi.org/10.1242/jcs.s1-3.10.97 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation CHRISTOPHER JOHNSTON; Original Communications: Auditory Apparatus of the Culex Mosquito. J Cell Sci 1 April 1855; s1-3 (10): 97–102. doi: https://doi.org/10.1242/jcs.s1-3.10.97 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All contentAll journalsJournal of Cell Science Search Advanced Search This content is only available via PDF. Copyright © 1855 by the Company of Biologists Ltd.1855 Article PDF first page preview Close Modal You do not currently have access to this content.
Two Different Isomers of Vitamin E Prevent Bone Loss in Postmenopausal Osteoporosis Rat Model (2012)

Postmenopausal osteoporotic bone loss occurs mainly due to cessation of ovarian function, a condition associated with increased free radicals. Vitamin E, a lipid-soluble vitamin, is a potent antioxidant which can scavenge free radicals in the body. In this study, we investigated the effects of alpha-tocopherol and pure tocotrienol on bone microarchitecture and cellular parameters in ovariectomized rats. Three-month-old female Wistar rats were randomly divided into ovariectomized control, sham-operated, and ovariectomized rats treated with either alpha-tocopherol or tocotrienol. Their femurs were taken at the end of the four-week study period for bone histomorphometric analysis. Ovariectomy causes bone loss in the control group as shown by reduction in both trabecular volume (BV/TV) and trabecular number (Tb.N) and an increase in trabecular separation (Tb.S). The increase in osteoclast surface (Oc.S) and osteoblast surface (Ob.S) in ovariectomy indicates an increase in bone turnover rate. Treatment with either alpha-tocopherol or tocotrienol prevents the reduction in BV/TV and Tb.N as well as the increase in Tb.S, while reducing the Oc.S and increasing the Ob.S. In conclusion, the two forms of vitamin E were able to prevent bone loss due to ovariectomy. Both tocotrienol and alpha-tocopherol exert similar effects in preserving bone microarchitecture in estrogen-deficient rat model.
A Placebo-Controlled Trial of Pioglitazone in Subjects with Nonalcoholic Steatohepatitis (2006)

In this proof-of-concept study, the administration of pioglitazone led to metabolic and histologic improvement in subjects with nonalcoholic steatohepatitis. Larger controlled trials of longer duration are warranted to assess the long-term clinical benefit of pioglitazone. (ClinicalTrials.gov number, NCT00227110 [ClinicalTrials.gov] .).