gebhart/patrepeval huggingface dataset:
The γ-Secretase Complex:  Membrane-Embedded Proteolytic Ensemble (2006)

Gamma-secretase is responsible for the proteolytic processing of a variety of membrane-associated fragments derived from type I integral membrane proteins, including the amyloid beta-protein precursor and the Notch receptor. This enzyme is composed of four different integral membrane proteins: presenilin, nicastrin, Aph-1, and Pen-2. During assembly and maturation of the protease complex, presenilin is endoproteolyzed into two subunits, each of which contributes one aspartate to the active site of an aspartyl protease. Substrate apparently interacts with an initial docking site before passing in whole or in part between the two presenilin subunits to the internal water-containing active site. The ectodomain of nicastrin also interacts with the N-terminus of the substrate as an essential step in substrate recognition and processing. Sites for allosteric regulation on the protease complex allow selective inhibition or modulation of APP processing without interfering with Notch signaling, and such selective agents may represent promising leads for the development of Alzheimer's disease therapeutics. Elucidation of detailed structural features of gamma-secretase and other membrane-embedded proteases is the next frontier in understanding how these enzymes carry out hydrolysis within the lipid bilayer.
Carbon capture with ionic liquids: overview and progress (2012)

As an emerging carbon dioxide (CO2) capture technology, separating CO2 from industrial gases with ionic liquids is increasingly attracting remarkable interests. In this paper, the research progress on CO2 capture with ionic liquids is reviewed with particular attention on the viewpoint of potential industrial applications. We investigate and compare the CO2 capture capacities of pure ionic liquids, including conventional and task-specific ionic liquids, and ionic liquid-based mixtures. The mechanisms of chemisorption and physisorption are explained with experimental characteristics and molecular simulation, which show more and more important roles for screening suitable ionic liquids from tremendous candidates. Considering the scaling up of novel units for ionic liquid-based fluids, the studies on the transport properties and hydrodynamics of ionic liquid fluids are presented. The process design and assessment of ionic liquid processes are discussed, including the research progress on thermodynamic properties and prediction models. Besides giving an overview of research for each issue above, we also provide discussions for future work to try to identify the gap between the current work and the demand by real industrial applications. Finally, we present some perspectives of ionic liquid-based novel technologies, and the challenges which will be faced while developing industrially available technologies.
Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins. (1987)

We have developed an in vitro assay which measures the ability of growth cones to extend on an axonal substrate. Neurite lengths were compared in the presence or absence of monovalent antibodies against specific neural cell surface glycoproteins. Fab fragments of antibodies against the neural cell adhesion molecule, NCAM, have an insignificant effect on the lengths of neurites elongating on either an axonal substrate or a laminin substrate. Fab fragments of polyclonal antibodies against two new neural cell surface antigens, defined by mAb G4 and mAb F11, decrease the lengths of neurites elongating on an axonal substrate, but have no effect on the lengths of neurites elongating on a laminin substrate. G4 antigen is related to mouse L1, while F11 antigen appears to be distinct from all known neural cell surface glycoproteins. Our results suggest that the G4 and F11 antigens help to promote the extension of growth cones on axons.
A low dark current InGaAs/InP p-i-n photodiode with covered mesa structure (1987)

A new InGaAs p-i-n photodiode with a covered mesa (CM) structure having extremely low dark current characteristics and high yields has been developed. The device consists of only two epitaxial layers: n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> -InP and n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> -InGaAs, continuously grown on an n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> -InP substrate by liquid-phase epitaxy. The InGaAs layer is chemically etched to be a tapered shape in order to make the fabrication process simple, as compared with a conventional mesa diode. The Zn diffusion to form a p-n junction is carried out without a diffusion mask such as Si <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> or SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> , which induces damage due to the thermal stress. The tapered-shape InGaAs layer is covered with the Zn-diffused layer because a surface p-n junction occurring in an InGaAs region is leaky. Therefore, the surface p-n junction of the photodiode appears in the n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> -InP layer, which has a bandgap about two times wider than the InGaAs. Finally, the passivation of the surface p-n junction is carried out with a Si <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> film formed by a plasma-assisted chemical vapor deposition. We have successfully achieved an extremely low dark current of 20 pA at a reverse bias voltage of 10 V and a high yield of 80 percent, by adopting the CM structure and the simple fabrication process.
Virtual sensor services for simulated mobile nodes (2017)

The faithful and flexible simulation of mobile robotic platforms with different application-specific sensing capabilities will become increasingly important in order to test a wide range of scenarios without having to run real missions. Current research focuses primarily on the mobility dynamics of such systems, and to this end a lot of work has already been done to support the simulation of various land, surface, underwater and aerial vehicles. We tackle a complementary aspect, namely that of sensing. More concretely, we propose to support virtual sensors via a service-oriented architecture, which cleanly separates a simulated vehicle from the logic that provides the (artificial) sensor values to it. The application running on the simulated vehicle has the illusion of accessing a real onboard sensor, but sensor data is provided by an independent software component, which can be flexibly placed on the same machine that runs the simulated vehicle, or on a remote host. This paper introduces the concept, describes a proof-of-concept implementation, and provides an evaluation that identifies the limits of the proposed approach.
Mechanism of initial rapid rate retardation in cellobiohydrolase catalyzed cellulose hydrolysis (2010)

Despite intensive research, the mechanism of the rapid retardation in the rates of cellobiohydrolase (CBH) catalyzed cellulose hydrolysis is still not clear. Interpretation of the hydrolysis data has been complicated by the inability to measure the catalytic constants for CBH-s acting on cellulose. We developed a method for measuring the observed catalytic constant (k(obs)) for CBH catalyzed cellulose hydrolysis. It relies on in situ measurement of the concentration of CBH with the active site occupied by the cellulose chain. For that we followed the specific inhibition of the hydrolysis of para-nitrophenyl-beta-D-lactoside by cellulose. The method was applied to CBH-s TrCel7A from Trichoderma reesei and PcCel7D from Phanerochaete chrysosporium and their isolated catalytic domains. Bacterial microcrystalline cellulose, Avicel, amorphous cellulose, and lignocellulose were used as substrates. A rapid decrease of k(obs) in time was observed on all substrates. The k(obs) values for PcCel7D were about 1.5 times higher than those for TrCel7A. In case of both TrCel7A and PcCel7D, the k(obs) values for catalytic domains were similar to those for intact enzymes. A model where CBH action is limited by the average length of obstacle-free way on cellulose chain is proposed. Once formed, productive CBH-cellulose complex proceeds with a constant rate determined by the true catalytic constant. After encountering an obstacle CBH will "get stuck" and the rate of further cellulose hydrolysis will be governed by the dissociation rate constant (k(off)), which is low for processive CBH-s.
Brain Phenotype of Transgenic Mice Overexpressing Cystathionine β-Synthase (2012)

We demonstrate that CBS overexpression has functional consequences on hippocampal neuronal networks. These results shed new light on the function of the CBS gene, and raise the interesting possibility that CBS overexpression might have an advantageous effect on some cognitive functions in DS.
Mechanism of Tau-Induced Neurodegeneration in Alzheimer Disease and Related Tauopathies (2008)

The accumulation of hyperphosphorylated tau is a common feature of several dementias. Tau is one of the brain microtubule-associated proteins. Here we discuss tau's function in microtubule assembly and stabilization and with regards to tau's interactions with other proteins, membranes, and DNA. We describe and analyze important posttranslational modifications: hyperphosphorylation, glycosylation, ubiquitination, glycation, polyamination, nitration, and truncation. We discuss how these post-translational modifications can alter tau's biological function and what is known about tau self-assembly, and we propose a mechanism of tau polymerization. We analyze the impact of natural mutations on tau that cause fronto-temporal dementia associated with chromosome 17 (FTDP-1 7). Finally, we consider whether tau accumulation or its conformational change is related to tau-induced neurodegeneration, and we propose a mechanism of neurodegeneration.
A 16b microprocessor with on chip memory protection (1983)

A 16b microprocessor, containing on-chip memory management and protection, which executes up to 1.5-million instructions per second,will be discussed. The chip (334×340 mils <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) with 120K transistors uses an 8MHz clock and provides a 242.5ns data access time at the pins of the chip.
O-Linked GlcNAc Transferase Is a Conserved Nucleocytoplasmic Protein Containing Tetratricopeptide Repeats (1997)

O-Linked GlcNAc addition and phosphorylation may compete for sites on nuclear pore proteins and transcription factors. We sequenced O-linked GlcNAc transferase from rabbit blood and identified the homologous Caenorhabditis elegans transferase gene on chromosome III. We then isolated C. elegans and human cDNAs encoding the transferase. The enzymes from the two species appear to be highly conserved; both contain multiple tetratricopeptide repeats and nuclear localization sequences. The C. elegans transferase accumulated in the nucleus and in perinuclear aggregates in overexpressing transgenic lines. O-Linked GlcNAc transferase activity was also elevated in HeLa cells transfected with the human cDNA. At least four human transcripts were observed in the tissues examined ranging in size from 4.4 to 9.3 kilobase pairs. The two largest transcripts (7.9 and 9.3 kilobase pairs) were enriched at least 12-fold in the pancreas. Based on its substrate specificity and molecular features, we propose that O-linked GlcNAc transferase is part of a glucose-responsive pathway previously implicated in the pathogenesis of diabetes mellitus.
Estructuras agrarias comparadas: la Puna argentina y el sur boliviano a comienzos del siglo XX (2005)

Este artículo tiene por propósito realizar un aporte más al conocimiento de la problemática socio-económica de la Puna argentina, específicamente a su estructura agraria, atendiendo los cambios y permanencias relativos a la propiedad y tenencia de la tierra, clave del poder económico y social y eje fundamental en las relaciones de producción de esta sociedad agraria. Se trata la cuestión en el marco de la problemática de las tierras indígenas, comparando la situación del Norte argentino con la del Sur boliviano, analizando las políticas de ambos Estados en torno a la tierra de las comunidades y sus efectos en las estructuras agrarias de comienzos del siglo XX.
Building blocks for computer vision systems (1993)

The designers and users of computer vision systems depend on a repertoire of software and hardware building blocks. This article reviews past and future trends in computer vision research and system development.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Negative Regulatory Element Involved in the Hormonal Regulation of GlcNAc-1-P Transferase Gene in Mouse Mammary Gland (1996)

The gene encoding UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1-phosphate transferase (GPT), the enzyme that initiates the pathway for the biosynthesis of asparagine-linked glycoproteins, is ubiquitously expressed in eukaryotic cells. However, its expression in the mammary gland is developmentally and hormonally regulated; transcription of the mouse mammary GPT gene is stimulated by the lactogenic hormones, insulin, glucocorticoid, and prolactin. The involvement of cisacting elements in regulating the expression of the mouse GPT (mGPT) gene was investigated by transient transfections of various GPT promoter/luciferase (Luc) constructs into primary mouse mammary epithelial cells. A series of 5'-deletions of the GPT promoter identified a distal negative regulatory region (base pairs -1057 to -968) and deletion of this region results in enhanced hormonal induction (approximately 7-fold) with no effect on basal promoter activity. Electrophoretic mobility shift assays (EMSA) performed with nuclear extracts from different developmental stages of mouse mammary gland demonstrated that the binding activity of the nuclear proteins to the distal negative regulatory region was predominant in virgin stage as compared with pregnant and lactating stages. EMSA performed with nuclear extracts from virgin explants showed that the binding activity was markedly decreased after cultivation with the combination of the three lactogenic hormones. DNase I footprinting analysis identified two pentamer direct repeat motifs, AGGAA and GAAAC, within the negative regulatory region. EMSA competition experiments showed that mutations within the direct repeats failed to compete for binding of the nuclear proteins to labeled wild type oligonucleotide. Transcription from the promoter containing the mutated direct repeats was increased greatly, consistent with the conclusion that these motifs functions in vivo to repress GPT gene expression. These data suggest the importance of the negative regulatory region in hormonal control of mGPT gene expression in mammary gland.
The Organophilic Bentonites. II. Organic Liquid Gels. (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Organophilic Bentonites. II. Organic Liquid Gels.J. W. Jordan, B. J. Hook, and C. M. FinlaysonCite this: J. Phys. Chem. 1950, 54, 8, 1196–1208Publication Date (Print):August 1, 1950Publication History Published online1 May 2002Published inissue 1 August 1950https://doi.org/10.1021/j150482a012RIGHTS & PERMISSIONSArticle Views255Altmetric-Citations104LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (645 KB) Get e-Alerts
Labelled dependencies in machine translation evaluation (2007)

We present a method for evaluating the quality of Machine Translation (MT) output, using labelled dependencies produced by a Lexical-Functional Grammar (LFG) parser. Our dependencybased method, in contrast to most popular string-based evaluation metrics, does not unfairly penalize perfectly valid syntactic variations in the translation, and the addition of WordNet provides a way to accommodate lexical variation. In comparison with other metrics on 16,800 sentences of Chinese-English newswire text, our method reaches high correlation with human scores.
Power line modems and networks (1989)

A look at the history, present applications and system designs for mains-borne data communications. Modern selection and networking software are covered.<
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Mutants of Escherichia coli heat-labile toxin lacking ADP-ribosyltransferase activity act as nontoxic, mucosal adjuvants. (1995)

A nontoxic mutant (LTK7) of the Escherichia coli heat-labile enterotoxin (LT) lacking ADP-ribosylating activity but retaining holotoxin formation was constructed. By using site-directed mutagenesis, the arginine at position 7 of the A subunit was replaced with lysine. This molecule, which was nontoxic in several assays, was able to bind to eukaryotic cells and acted as a mucosal adjuvant for co-administered proteins; BALB/c mice immunized intranasally with LTK7 and ovalbumin developed high levels of serum and local antibodies to ovalbumin and toxin. In addition, mice immunized intranasally with fragment C of tetanus toxin and LTK7 were protected against lethal challenge with tetanus toxin. Thus nontoxic mutants of heat-labile toxin can act as effective intranasal mucosal adjuvants.
Role of src-like protooncogenes in lymphocyte proliferation. (1991)

Cross-linking of membrane-bound immunoglobulins, which are B cell antigen receptors, causes proliferation and differentiation of B cells or the inhibition of their growth. The receptor-mediated signalling involves tyrosine phosphorylation of cellular proteins. The Src-like protein-tyrosine kinase Lyn is expressed preferentially in B cells and is an intracytoplasmic constituent of the B cell antigen receptor complex. Cross-linking of membrane-bound immunoglobulin M with antibody induced rapid increases in the kinase activities of Lyn and Lyn-associated phosphatidylinositol-3 kinase. Cross-linking of B-cell antigen receptor also induced association of Lyn with an 85 kDa non-catalytic subunit of phosphatidylinositol-3 kinase. Thus, Lyn is functionally associated with membrane-bound immunoglobulin M, and seems to participate in B cell antigen receptor-mediated signalling. On the other hand, accumulating evidence shows that Fyn and Lck are involved in T-cell activation. Co-immunoprecipitation experiments showed that Fyn was physically associated with T cell antigen receptor-CD3 complex. Transient expression of actively mutated Fyn, having Phe-528 instead of Tyr-528, in Jurkat T cells stimulated the fos promoter and serum response element (SRE), suggesting that the Fyn kinase stimulates c-fos expression through SRE. An analogous set of experiments showed that introduction of the active Fyn alone had little effect on IL-2 promoter. However, it could stimulate transcription from this promoter when transfected cells were stimulated by a combination of Concanavalin A (ConA) and 12-O-tetradecanoylphorbol-13-acetate (TPA). Under the same conditions, normal Lck and Lyn could not stimulate IL-2 promoter.
The Tug function: A Method of context sensitive dot structuring for digital halftones (1990)

The process of digitizing images to create halftones inherently reduces sharpness through the averaging of grayness values within cell areas. Within the context of a resolution independent page description language, overcoming this reduced sharpness is conventionally addressed by adjusting the grayness values of cells to create larger or smaller halftone dots where edges are present. Such an approach does not take full advantage of the capabilities of the output device. The objective of this project was to design and implement a method of sharpening digital images by altering the shape and position, rather than the size, of halftone dots. Such a method can more accurately represent the original image and more closely emulate the characteristics of photomechanically produced halftones. Within the PostScript page description language, the generation of halftone dots is controlled by the spot function. A particular type of spot function, the Tug function, was developed to control the shape and position of halftone dots based on the grayness value of each cell and its surrounding neighbors. Because the standard PostScript imaging operators are not designed to allow halftone dot shapes to be redefined on a cell-by-cell basis, an alternate method of generating images was created. A computer program in the PostScript programming language was written to perform the requisite image analysis and halftone dot structuring to drive a laser printing device for viewing the effects of the Tug function. Three representative photographic images were processed in this manner. A panel of judges compared the resulting prints with control prints processed without the Tug function but by the same imaging method. The judges' subjective preferences are presented, and the relative merits of the Tug function are discussed.
Constitutive expression of B7 restores immunogenicity of tumor cells expressing truncated major histocompatibility complex class II molecules. (1993)

The inability of the autologous host to reject resident tumor cells is frequently the result of inadequate generation of tumor-specific T cells. Specific activation of T cells occurs after delivery of two signals by the antigen-presenting cell. The first signal is antigen-specific and is the engagement of the T-cell antigen receptor by a specific major histocompatibility complex antigen-peptide complex. For some T cells, the second or costimulatory signal is the interaction of the T-cell CD28 receptor with the B7 activation molecule of the antigen-presenting cell. In the present study, we demonstrate that mouse sarcoma cells genetically engineered to provide both T-cell activation signals stimulate potent tumor-specific CD4+ T cells that cause rejection of both engineered and wild-type neoplastic cells. Two other recent studies have also demonstrated that costimulation via B7 can improve tumor immunity. However, our study differs from these reports by two important observations. (i) One of these studies utilized mouse tumor cells expressing xenogenic viral antigens, and hence, the results are not applicable to wild-type resident tumors. Our study, however, demonstrates that coexpression of B7 by major histocompatibility complex class II+ tumor cells induces immunity in the autologous host that is specific for naturally occurring tumor antigens of poorly immunogenic tumors. (ii) In both earlier studies, only CD8+ T cells were activated after coexpression of B7, whereas in the present report, tumor-specific CD4+ T cells are generated. This report therefore illustrates the role of B7 activation molecule in stimulating potent tumor-specific CD4+ T cells that mediate rejection of wild-type tumors and provides a theoretical basis for immunotherapy of established tumors.