gebhart/patrepeval huggingface dataset:
Central venous pressure: principles, measurement, and interpretation. (2012)

Central venous pressure (CVP), an estimate of right atrial pressure, has been used to assess cardiac preload and volume status in critically ill patients, assist in the diagnosis of right-sided heart failure, and guide fluid resuscitation. It is determined by the interaction between cardiac function and venous return. CVP measurements are relatively easy to obtain; however, because of the complex relationship between CVP, cardiac output, and the vascular system, they may be difficult to interpret. This article reviews the physiologic principles of CVP, indications for its use, technical aspects and pitfalls of CVP measurements, and interpretation of the information obtained.
An ethanol/ether soluble apoprotein from rat lung surfactant augments liposome uptake by isolated granular pneumocytes. (1984)

Ethanol/ether soluble apoproteins, comprising 17% of the total recovered surfactant-associated proteins, were isolated from rat lung surfactant and purified by silicic acid chromatography. The protein that eluted in 4:1 chloroform/methanol accounted for greater than 85% of protein in the ethanol/ether soluble fraction and was termed surfactant apoprotein Et (Apo Et). By sodium dodecyl sulfate polyacrylamide gel electrophoresis, this protein had an apparent molecular weight of approximately 10,500. Apo Et was evaluated for its effect on uptake of synthetic phospholipids in liposomal form by isolated granular pneumocytes (Type II alveolar epithelial cells) in primary culture. Liposomes were prepared to approximate the phospholipid composition of the alveolar surfactant, and uptake was measured by the accumulation of the radioactively labeled dipalmitoyl phosphatidyl choline fraction. The uptake of liposomal phosphatidylcholine by cells incubated for 2 h with Apo Et was increased by 61% over control. Most of the cell-associated phospholipid uptake was resistant to treatment with trypsin, suggesting an increased internalization of liposomal material in the presence of Apo Et. The effect of Apo Et on uptake was concentration and time dependent and was not associated with cell damage, phospholipase activity, or detergent properties of the protein. Apo Et had no significant effect on phosphatidylcholine uptake by granular pneumocytes maintained for 7 d in primary culture. Apo Et augmented the uptake of phospholipids by alveolar macrophages although total uptake by these cells was less than that observed with granular pneumocytes. Because Apo Et increases the rate of uptake of surfactant phospholipids by alveolar cells (granular pneumocytes and alveolar macrophages), this protein may represent a physiologically important regulator for clearance of lung surfactant phospholipids.
Robust distributed model predictive control (2007)

This paper presents a formulation for distributed model predictive control (DMPC) of systems with coupled constraints. The approach divides the single large planning optimization into smaller sub-problems, each planning only for the controls of a particular subsystem. Relevant plan data is communication between sub-problems to ensure that all decision satisfy the coupled constraints. The new algorithm guarantees that all optimizations remain feasible, that the coupled constraints will be satisfied, and that each subsystem will converge to its target, despite the action of unknown but bounded disturbances. Simulation results are presented showing that the new algorithm offers significant reductions in computation time for only a small degradation in performance in comparison with centralized MPC.
High-reflectivity monomode-fibre grating filters (1986)

The fabrication and characterisation of efficient, narrowband fibre-optic filters utilising surface-relief gratings etched into monomode fibre are described. A device with 92% reflectivity and 1·8 nm bandwidth at 1·3 μm wavelength using a first-order Bragg grating with a high-index overlayer is reported.
RF sampling gates: a brief review (1986)

The paper contains a brief review of the various techniques employed for the sampling of RF and microwave signals, and reference is made to subharmonic sampling as one of the possible applications. Particular attention is given to the two- and four-diode gate arrangements and the associated pulse strategies for fast switching time are discussed, as are the theoretical and practical aspects associated with the use of finite risetime sampling pulse trains. The design and operation of an experimental 10 GHz GaAs FET unit are given in detail, and recommendations are given in the concluding Section as regards development effort in sampling gates.
A glutathione conjugate of hepoxilin A3: formation and action in the rat central nervous system. (1990)

Incubation of (8R)- and (8S)-[1-14C]hepoxilin A3 [where hepoxilin A3 is 8-hydroxy-11,12-epoxyeicosa-(5Z,9E,14Z)-trienoic acid] and glutathione with homogenates of rat brain hippocampus resulted in a product that was identified as the (8R) and (8S) diastereomers of 11-glutathionyl hepoxilin A3 by reversed-phase high performance liquid chromatographic comparison with the authentic standard made by total synthesis. Identity was further confirmed by cleavage of the isolated product with gamma-glutamyltranspeptidase to yield the corresponding cysteinylglycinyl conjugate that was identical by reversed-phase high performance liquid chromatographic analysis with the enzymic cleavage product derived from the synthetic glutathionyl conjugate. The glutathionyl and cysteinylglycinyl conjugate are referred to as hepoxilin A3-C and hepoxilin A3-D, respectively, by analogy with the established leukotriene nomenclature. Formation of hepoxilin A3-C was greatly enhanced with a concomitant decrease in formation of the epoxide hydrolase product, trioxilin A3, when the epoxide hydrolase inhibitor trichloropropene oxide was added to the incubation mixture demonstrating the presence of a dual metabolic pathway in this tissue involving hepoxilin epoxide hydrolase and glutathione S-transferase processes. Hepoxilin A3-C was tested using intracellular electrophysiological techniques on hippocampal CA1 neurons and found to be active at concentrations as low as 16 nM in causing membrane hyperpolarization, enhanced amplitude and duration of the post-spike train afterhyperpolarization, a marked increase in the inhibitory postsynaptic potential, and a decrease in the spike threshold. These findings suggest that these products in the hepoxilin pathway of arachidonic acid metabolism formed by the rat brain may function as neuromodulators.
A review of the recent development of MEMS and crystal oscillators and their impacts on the frequency control products industry (2008)

Due to their high Q and temperature-stable properties, quartz crystal oscillators are important clock sources in consumer, commercial, industrial, and military products for many years. The demand for quartz crystals and crystal oscillators has been increasing steadily between 4 and 10% annually since the ldquodotcomrdquo market collapse in 2000~2001. The total market for 2008 is expected to exceed $4.1B. The quartz crystal and crystal oscillator industry has made major progresses in miniaturization, performance enhancement, and cost reduction in the past ten years. The unique fabrication and encapsulation requirements though render quartz crystals and crystal oscillators difficult or close to impossible to be integrated onto the silicon-based IC platforms. The recent strong marketing push of the all silicon MEMS resonators and oscillators seemed to re-ignite the interest in displacing the quartz crystal technology and to open up again the prospect in clock source integration. Based on a 2006 review paper, <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">[1]</sup> the author expands on the subject by reviewing the development of the all silicon MEMS oscillators and crystal oscillators in the past few years and commenting on what challenges they face in the highly competitive frequency control products industry. Since information on the technical development of the all silicon MEMS oscillators and crystal oscillators is abundantly and readily available, this paper addresses more on the market and business aspects. This paper also touches on the recent development of piezoelectric-activated silicon MEMS resonators and oscillators and all silicon oscillators (with no moving parts).
Spatially independent activity patterns in functional MRI data during the Stroop color-naming task (1998)

A method is given for determining the time course and spatial extent of consistently and transiently task-related activations from other physiological and artifactual components that contribute to functional MRI (fMRI) recordings. Independent component analysis (ICA) was used to analyze two fMRI data sets from a subject performing 6-min trials composed of alternating 40-sec Stroop color-naming and control task blocks. Each component consisted of a fixed three-dimensional spatial distribution of brain voxel values (a "map") and an associated time course of activation. For each trial, the algorithm detected, without a priori knowledge of their spatial or temporal structure, one consistently task-related component activated during each Stroop task block, plus several transiently task-related components activated at the onset of one or two of the Stroop task blocks only. Activation patterns occurring during only part of the fMRI trial are not observed with other techniques, because their time courses cannot easily be known in advance. Other ICA components were related to physiological pulsations, head movements, or machine noise. By using higher-order statistics to specify stricter criteria for spatial independence between component maps, ICA produced improved estimates of the temporal and spatial extent of task-related activation in our data compared with principal component analysis (PCA). ICA appears to be a promising tool for exploratory analysis of fMRI data, particularly when the time courses of activation are not known in advance.
Parallel processing gets down to business (1985)

While the ultimate aim of most parallel processor designers is to create extremely powerful machines that will out shine current supercomputers such as those from Cray and Control Data (both in Minneapolis), most of the initial parallel machines will compete against supermini computers like DEC's VAX-11/780. The supermini market is potentially much larger than the specialized supercomputer field, and the first versions of most parallel machines don't yet equal the performance of the supercomputers. Still, the likely applications for future parallel processors are similar to those currently addressed by the supercomputers. The computers will be used by scientists doing simulations of weather and other complex processes, by engineers studying aerodynamic flows, and by artificial intelligence researchers designing expert systems. 5 references, 4 figures.
Different 3′ untranslated regions target alternatively processed hu-li tai shao (hts) transcripts to distinct cytoplasmic locations during Drosophila oogenesis (1999)

Cytoplasmic mRNA localization is one method by which protein production is restricted to a particular intracellular site. We report here a novel mechanism for localization of transcripts encoding distinct protein isoforms to different destinations. Alternative processing of transcripts produced in the Drosophila ovary by the hu-li tai shao (hts) locus introduces distinct 3' untranslated regions (3'UTRs) that differentially localize the mRNAs. Three classes of hts mRNA (R2, N32 and N4) are synthesized in the germ line nurse cells and encode proteins with adducin-homologous amino-terminal regions but divergent carboxy-terminal domains. The R2 and N32 classes of mRNA remain in the nurse cells and are not transported into the oocyte. In contrast, the N4 class of transcripts is transported from the nurse cells into the oocyte starting at stage 1, is subsequently localized to the oocyte cortex at stage 8 and then to the anterior pole from stage 9 on. All aspects of N4 transcript transport and localization are directed by the 345-nucleotide(nt)-long 3' untranslated region (3'UTR). The organization of localization elements in the N4 3'UTR is modular: a 150 nt core is sufficient to direct transport and localization throughout oogenesis. Additional 3'UTR elements function additively together with this core region at later stages of oogenesis to maintain or enhance anterior transcript anchoring. The swallow locus is required to maintain hts transcripts at the anterior pole of the oocyte and functions through the N4 3'UTR. In addition to the three classes of germ line-expressed hts transcripts, a fourth class (R1) is expressed in the somatic follicle cells that surround the germ line cells. This transcript class encodes the Drosophila orthologue of mammalian adducin.
Clinical and Genetic Abnormalities in Patients with Friedreich's Ataxia (1996)

The clinical spectrum of Friedreich's ataxia is broader than previously recognized, and the direct molecular test for the GAA expansion on chromosome 9 is useful for diagnosis, determination of prognosis, and genetic counseling.
Ataxin-3 protein modification as a treatment strategy for spinocerebellar ataxia type 3: Removal of the CAG containing exon (2013)

Spinocerebellar ataxia type 3 is caused by a polyglutamine expansion in the ataxin-3 protein, resulting in gain of toxic function of the mutant protein. The expanded glutamine stretch in the protein is the result of a CAG triplet repeat expansion in the penultimate exon of the ATXN3 gene. Several gene silencing approaches to reduce mutant ataxin-3 toxicity in this disease aim to lower ataxin-3 protein levels, but since this protein is involved in deubiquitination and proteasomal protein degradation, its long-term silencing might not be desirable. Here, we propose a novel protein modification approach to reduce mutant ataxin-3 toxicity by removing the toxic polyglutamine repeat from the ataxin-3 protein through antisense oligonucleotide-mediated exon skipping while maintaining important wild type functions of the protein. In vitro studies showed that exon skipping did not negatively impact the ubiquitin binding capacity of ataxin-3. Our in vivo studies showed no toxic properties of the novel truncated ataxin-3 protein. These results suggest that exon skipping may be a novel therapeutic approach to reduce polyglutamine-induced toxicity in spinocerebellar ataxia type 3.
The Bacterial Cell Envelope (2010)

The bacteria cell envelope is a complex multilayered structure that serves to protect these organisms from their unpredictable and often hostile environment. The cell envelopes of most bacteria fall into one of two major groups. Gram-negative bacteria are surrounded by a thin peptidoglycan cell wall, which itself is surrounded by an outer membrane containing lipopolysaccharide. Gram-positive bacteria lack an outer membrane but are surrounded by layers of peptidoglycan many times thicker than is found in the gram-negatives. Threading through these layers of peptidoglycan are long anionic polymers, called teichoic acids. The composition and organization of these envelope layers and recent insights into the mechanisms of cell envelope assembly are discussed.
Solution structure of a short DNA fragment studied by neutron scattering (1986)

The solution structure of a DNA fragment of 130 base pairs and known sequence has been investigated by neutron small-angle scattering. In 0.1 M NaCl, the overall structure of the DNA fragment which contains the strong promoter A1 of the Escherichia coli phage T7 agrees with that expected for B-DNA. The neutron scattering curve is well fitted by that of a rigid rod with a length of 44 nm and a diameter of 2 nm. The results were confirmed by quasi-elastic light scattering and analytical centrifugation. The neutron measurements in H2O and D2O buffer reveal a cross-sectional inhomogeneity not detected by X-ray small-angle scattering. This inhomogeneity is caused by the hydration layer around the DNA core and not by the helical structure. The primary solvent shell has a density increased by at least 4-9% compared to bulk water.
Defect-Tolerant Single-Electron Charging at Room Temperature in Metal Nanoparticle Decorated Biopolymers (2001)

Gold nanoparticles assembled on a biopolymer template (see Figure) between metal electrodes on an insulating substrate are shown to exhibit unambiguous single electron charging effects that are found to depend on the nanoparticle properties and the geometrical contraints imposed by the biopolymer. The results support the idea of using nanoparticles in conjuction with biomolecular organization to produce nanoscale systems with defect-tolerant current–voltage behavior.
Hydrazide oligonucleotides: new chemical modification for chip array attachment and conjugation (2002)

We report the synthesis of new phosphoramidite building blocks and their use for the modification of oligonucleotides with hydrazides. The reaction of these hydrazide oligonucleotides with active esters and aldehydes is demonstrated for solution conjugation and immobilization. Compared with the established amino modified oligonucleotides, hydrazides show enhanced reactivity at neutral and acidic buffer conditions. One method to introduce hydrazides is using amidites with preformed, protected hydrazides. A completely novel approach is the generation of the hydrazide functionality during the oligonucleotide cleavage and deprotection with hydrazine. Therefore, building blocks for the introduction of esters as hydrazide precursors are described. For the enhanced attachment on surfaces branched modifier amidites, which introduce up to four reactive groups to the oligonucleotide, are applied. The efficiency of branched hydrazide oligonucleotides compared with standard amino modified oligonucleotides for the immobilization of DNA on active electronic Nanogen chips is demonstrated.
Superior Capacity Retention Spinel Manganese Oxyfluoride Cathodes for Lithium Ion Batteries (2006)

Abstract not Available.
Investigation of the role of microtubules in protein secretion from lactating mouse mammary epithelial cells (1992)

Disruption of microtubules has been shown to reduce protein secretion from lactating mammary epithelial cells. To investigate the involvement of microtubules in the secretory pathway in these cells we have examined the effect of nocodazole on protein secretion from mammary epithelial cells derived from the lactating mouse. Mouse mammary cells have extensive microtubule networks and 85% of their tubulin was in a polymeric form. Treatment with 1 micrograms/ml nocodazole converted most of the tubulin into a soluble form. In a continuous labelling protocol it was found that nocodazole did not interfere with protein synthesis but over a 5 h period secretion was markedly inhibited. To determine whether the inhibition was at the level of early or late stages of the secretory pathway mammary cells were pulse-labelled for 1 h to label protein throughout the secretory pathway before nocodazole treatment. When secretion was subsequently assayed it was found to be slower and only partially inhibited. These findings suggest that the major effect of nocodazole is on an early stage of the secretory pathway and that microtubules normally facilitate vesicle transport to the plasma membrane. An involvement of microtubules in vesicle transport to the plasma membrane is consistent with an observed accumulation of casein vesicles in nocodazole-treated cells. Exocytosis stimulated by the calcium ionophore ionomycin was unaffected by nocodazole treatment. We conclude from these results that the major effect of nocodazole is at an early stage of the secretory pathway, one possible target being casein vesicle biogenesis in the trans-Golgi network.
Differential activation requirements for virgin and memory T cells. (1988)

Most studies of the activation requirements for T cells have used either T cell lines or populations of normal T cells that consist of a mixture of virgin and Ag-primed T cells. These two subpopulations of T cells can now be distinguished in humans by their reactivity with mAb. The anti-CD45R antibody HB10 identifies virgin T cells (T degrees) that are non-reactive to recall Ag and relatively poor at providing help for B cell differentiation. Conversely, memory T cells (T') that can react to recall Ag and enhance Ig production are non-reactive with anti-CD45R, but can be identified with the UCHL1 antibody. We have used these antibodies to separate the T degrees and T' populations and examine their activation requirements. On activation CD45R+ cells rapidly began to lose the CD45R Ag and express the UCHL1 Ag in increased amounts, whereas the UCHL1+ cells retained this phenotype. Both populations responded to PHA in the presence of monocytes, but when triggered by an antibody to CD3 only the T' cells were induced to express IL-2R, produce IL-2, and to proliferate. The T degrees population of cells remained relatively quiescent by all of these parameters. However, anti-CD3 stimulation conditioned the T degrees cells for IL-2 responsiveness, inasmuch as the addition of rIL-2 resulted in significant IL-2R expression and proliferation. When the CD4+ T degrees and CD4+ T' subpopulations were isolated and examined in the same assays similar results were obtained. The data indicate that fundamental differences exist in the triggering requirements for T degrees and T' cells.
A Deletion Mutation within the Ligand Binding Domain Is Responsible for Activation of Epidermal Growth Factor Receptor Gene in Human Brain Tumors (1990)

Two transplantable cell lines of human glioblastoma multiforme GL-3 and GL-5 carried an amplification and overexpression of structurally altered epidermal growth factor (EGF) receptor gene: the 140 kilodalton EGF receptors in these cases exhibited a constitutively expressed tyrosine kinase activity without the ligand. Here, we isolated the abnormal EGF receptor cDNA from GL-5 cell line, and demonstrated that this cDNA bears a single large intramolecular deletion mutation 801 base pairs long within the ligand binding domain of EGF receptor. In other regions no amino acid substitution was observed. At the level of genomic DNA, this deletion appeared to start from the 1st intron and terminate in the 6th intron of the EGF receptor gene. However, in the two lines of glioblastoma, GL-3 and GL-5, the positions of the start or the end of the deletion mutation in these introns were not identical, suggesting an involvement of a unique recombination mechanism in the formation of deletion mutation. A weak but ligand-independent transforming activity was observed in the deletion-carrying EGF receptor cDNA.
New mobile payment protocol: Mobile pay center protocol 2 (MPCP2) by using new key agreement protocol: VAM (2011)

Growing of wireless networks and popularity of mobile devices represents an incredible opportunity to empower them as a payment device. Unfortunately, some problems hindering the widespread acceptance of mobile payment for example: accountability properties, privacy protection, limitation of wireless networks and mobile devices. Recently, many public-key cryptography protocols have been presented for mobile payment. However, limited capabilities of mobile devices and wireless networks make these protocols unsuitable for mobile network. Moreover, these protocols were designed to preserve traditional flow of payment data, which is vulnerable to attack and increase the user's risk. In this paper, we propose a private mobile payment protocol which is based on client centric model and works by employing symmetric key operations. The proposed mobile payment protocol not only minimizes the computational operations and communications between the engaging parties, but also achieves a completely privacy protection for the payer, avoid repudiating transaction from each of them and decrease risk of replay attacks. In according to limitation of wireless networks and mobile devices, this paper recommends VAM key agreement protocol for generating shared session key between two parties instead of Diffie-Hellman.