gebhart/patrepeval huggingface dataset:
High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells (1992)

Sindbis virus is an alphavirus with a very wide host range, being able to infect many birds and mammals as well as mosquitoes. We have isolated a monoclonal antibody that largely blocks virus binding to mammalian cells. This antibody was found to be directed against the C-terminal domain of the high-affinity laminin receptor, a 67-kDa protein present on the cell surface that binds with high affinity to basement membrane laminin and that is known to be important in development and in tumor invasion. This receptor is believed to be formed from a 295-amino-acid polypeptide that is modified in some unknown way after translation. The primary sequence of this 295-amino-acid protein is highly conserved among mammals. We found the hamster amino acid sequence to be identical to a mouse sequence and to differ at only two amino acids from a human sequence and at two amino acids from a bovine sequence. To verify the importance of the laminin receptor for infection by Sindbis virus, hamster cells were stably transfected with the gene encoding the 295-amino-acid protein under the control of a high-efficiency promoter. Such transfected hamster cells overexpressed the laminin receptor at the cell surface, bound severalfold more Sindbis virions than did the parental cells, and became infected by Sindbis virus with a higher efficiency. In contrast, cells transfected with the antisense gene expressed less laminin receptor on the surface and were less susceptible to the virus. Binding of the virus varied linearly with the amount of laminin receptor on the cell surface, whereas infectivity measured with a plaque assay varied with the 1.4 power of the receptor concentration, suggesting that interaction with more than one receptor aids virus penetration. By these criteria, the laminin receptor functions as the major receptor for Sindbis virus entry into mammalian cells. We also found that the anti-laminin receptor antibody partially blocked Sindbis virus binding to mosquito cells, suggesting that the laminin receptor is conserved in mosquitoes and functions as a Sindbis virus receptor in this host. The wide distribution of this highly conserved receptor may be in part responsible for the broad host range exhibited by the virus, which infects a wide range of mammals and birds as well as its mosquito vector and can infect many different tissues within these hosts.(ABSTRACT TRUNCATED AT 400 WORDS)
Speech and speaker independent codebook design in VQ coding schemes (2005)

In vector quantization schemes usually speech and speaker dependent codebooks are applied in order to achieve good speech quality at medium bit rates. This paper deals with another approach: The speech waveforms are transformed into signals which ideally do no longer contain speech and speaker specific features. Thus these signals can be encoded by an universal vector quantizer. This concept is realized by a system called RELP-VQ. The performance of this RELP-VQ scheme was evaluated by SNR-measurements as well as by informal listening tests including female and male English and German speakers.
A Traceless Vascular‐Targeting Antibody–Drug Conjugate for Cancer Therapy (2011)

Right on target: A chemically defined vascular-targeting antibody–drug conjugate (ADC) that offers comprehensive tumor coverage has been developed. When injected intravenously, this ADC potently inhibited tumor growth in a syngeneic immunocompetent model of murine cancer which cannot be cured by conventional cytotoxic agents. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Cellular and viral splicing factors can modify the splicing pattern of CFTR transcripts carrying splicing mutations (2000)

Variable levels of aberrantly spliced cystic fibrosis transmembrane conductance regulator (CFTR ) transcripts were suggested to correlate with variable cystic fibrosis (CF) severity. We studied the effect of the cellular splicing factors, hnRNP A1 and ASF/SF2, and their adenoviral analogues, E4-ORF6 and E4-ORF3, that promote exon skipping and/or exon inclusion, on the splicing pattern of the CFTR mutation 3849+10kb C-->T and the 5T allele. These mutations can lead to cryptic exon inclusion and exon skipping, respectively. Overexpression of the cellular factors promoted exon skipping of pre-mRNA transcribed from minigenes carrying the mutation (p5T or p3849M). This led to a substantial decrease in the level of correctly spliced mRNA transcribed from p5T and generated correctly spliced mRNA transcribed from p3849M that was not found without overexpression of the factors. The viral factor, E4-ORF3, promoted exon inclusion and led to a substantial increase of the correctly spliced mRNA transcribed from the p5T. The factor, E4-ORF6, activated exon skipping and generated correctly spliced mRNA transcribed from p3849M. Thus, overexpression of alternative splicing factors can modulate the splicing pattern of CFTR alleles carrying splicing mutations. These results are important for understanding the mechanism underlying phenotypic variability in CF and other genetic diseases.
Generative Multi-Adversarial Networks (2016)

Generative adversarial networks (GANs) are a framework for producing a generative model by way of a two-player minimax game. In this paper, we propose the \emph{Generative Multi-Adversarial Network} (GMAN), a framework that extends GANs to multiple discriminators. In previous work, the successful training of GANs requires modifying the minimax objective to accelerate training early on. In contrast, GMAN can be reliably trained with the original, untampered objective. We explore a number of design perspectives with the discriminator role ranging from formidable adversary to forgiving teacher. Image generation tasks comparing the proposed framework to standard GANs demonstrate GMAN produces higher quality samples in a fraction of the iterations when measured by a pairwise GAM-type metric.
Cochrane Review Brief: Chinese Herbal Medicine for Endometriosis (2014)

Keywords: Chinese herbal medicine; endometriosis; gestrinone; danazolReview question:What is the effectiveness and safety of Chinese herbal medicine (CHM), compared with conventional treatment, on endometriosis-related pain and infertility in women with endometriosis?Nursing Implications:Endometriosis is a disease characterized by the presence of tissue that is morphologically and biologically similar to normal endometrium in locations outside the uterine cavity. Primary symptoms of endometriosis are endometriosis-related pain and infertility. The conventional treatment of endometriosis involves surgery and hormonal drugs, with short term benefits and high rates of rates of symptom recurrence (or return).In China, treatment of endometriosis using CHM is routine. CHM is thought to act via improvement of blood circulation and anti-inflammatory activity. There is considerable anecdotal evidence supporting the use of CHM as a potential treatment for endometriosis. Analyzing the evidence on the effectiveness and safety of CHM in the treatment of endometriosis can promote the development of treatment strategies for endometriosis which may reduce the suffering of patients and improve their quality of life.Study Characteristics:The review included two small, Chinese randomized controlled trials (RCTs), with a total of 158 women aged 23 to 45 years and an average age of 30 years. All participants had a laparoscopically confirmed diagnosis of endometriosis.The two trials, conducted by the same researchers, took place in a hospital outpatient department in China. One trial compared CHM endometriosis pills plus CHM enema (24 participants) and CHM endometriosis pills only (16 participants) with danazol (18 participants), and the other compared CHM endometriosis pills plus a herbal enema (48 participants) with gestrinone (52 participants).Treatment durations were for 3 months. One trial followed up for 1-24 months, while the other one did not follow up. The primary outcome was relief of endometriosis-related pain (both in the long term and short term) while secondary outcomes included improvement in fertility rates (live birth or pregnancy); reduction in the size and extent of endometrial cysts; improvement in quality of life scores; improvement of endometriosis-related symptoms apart from pain (e.g. fatigue); adverse effects resulting from the CHM intervention and rates of reoccurrenceand adverse effects.Both trials described adequate randomization and allocation concealment methods. The trials also reported single blinding for participants, and assessor blinding. Few or no participants were excluded from the analysis. Both studies were evaluated as a moderate risk of bias. …
Evaluation of Oligonucleotides Containing Two Novel 2′-O-Methyl Modified Nucleotide Monomers: A 3′-C-Allyl and a 2′-O-3′-C-Linked Bicyclic Derivative (1999)

The two ribo-configured nucleosides 1-(3-C-allyl-2-O-methyl-beta-D-ribo-pentofuranosyl)thymine 3 and (1S,5R,6R,8R)-5-hydroxy-6-(hydroxymethyl)-1-methoxy-8-(thymin-1-yl )- 2,7-dioxabicyclo[3.3.0]octane 6 have been transformed into their corresponding phosphoramidites, 5 and 8 respectively, and used as building blocks for the synthesis of modified oligonucleotides. The oligonucleotides were shown to hybridize with decreased binding affinity towards complementary single stranded DNA and RNA.
Promotion of intramuscular fat accumulation in porcine muscle by nutritional regulation (2011)

Recently, pork with marbling has received attention as good quality pork and scientists are required to develop methods to produce pork with reasonable amounts of intramuscular fat (IMF). The aim of this review is to describe studies relevant to promotion of IMF accumulation in porcine muscle by nutritional regulation. The main focus is on effect of dietary lysine levels. First, we found dietary low lysine up-regulated glucose transporter protein 4 messenger (m)RNA expression in Longissimus dorsi (L. dorsi) and Rhomboideus muscles. In addition, the proportion of oxidative fiber of both muscles was also enhanced by dietary low lysine. Because it has been observed that higher oxidative capacity is associated with higher IMF content, we hypothesized that dietary low lysine would promote IMF accumulation. Further, higher mRNA abundance of peroxisome proliferator-activated receptor γ, a master regulator of adipogenesis, in both muscles induced by dietary low lysine, supported this hypothesis. Indeed, IMF content of L. dorsi muscle of finishing pigs given a low lysine diet for 2 months until reaching the market weight was twice that of pigs given a control diet. Possible underlying mechanisms of IMF accumulation in porcine muscle and future perspectives are also discussed in this review.
A Soluble Recombinant Factor VIII Fragment Containing the A2 Domain Binds to Some Human Anti-Factor VIII Antibodies that Are not Detected by Immunoblotting (1992)

Human factor VIII (fVIII) inhibitors are pathologic antibodies that inactivate fVIII. A cDNA clone was modified to encode fVIII amino acid residues 373-740 for expression in a baculovirus vector in insect cells. The encoded protein fragment H2 was produced as a soluble, secreted protein, and it was used to test inhibitor plasmas for the presence of antibodies that were not detected by immunoblotting. Seven of 13 inhibitors that bound only to the fVIII light chain by immunoblotting also bound to fragment H2 in an immunoprecipitation assay. Thus multi-chain inhibitor reactivity of inhibitors is more frequent than previously reported. One of these inhibitors was shown to share the epitope for other inhibitors that bind to H2 within amino acid residues 373-541 in immunoblotting assays. The sensitive immunoprecipitation assay described allows determination of relative H2 binding capacity of the total IgG and epitope localization of inhibitors that cannot be similarly characterized by immunoblotting.
Optical versus electronic switching for broadband networks (1996)

The authors compare the use of electronic and optical switching for broadband networks. Their technological merits and their ability to meet traffic demand when multimedia communications become ubiquitous are considered. The authors conclude that electronic ATM switches are adequate and commercially mature for local access. An ATM-mesh switch with multihop routing can be used to achieve capacity of several hundreds of gigabits per second. Beyond access, the authors argue that traffic aggregation may allow switching to be performed on much longer time scales than that of ATM cells. Wavelength and mechanical fiber switching may be used economically for trunk switching, avoiding the expensive processes of optoelectronic conversions and demultiplexing.
The Effects of Augmentation with Swine Small Intestine Submucosa on Tendon Healing under Tension (2005)

Enhancing the biological process of tendon healing under tension by using a collagen matrix patch may improve the ultimate success of rotator cuff repair.
Deep venous thrombosis of the lower extremity: US evaluation. (1987)

The sensitivity of duplex ultrasonography (US) for detecting deep venous thrombosis of the lower extremity was compared with that of venography in a prospective study of 54 patients. Doppler analysis of the common femoral vein and US imaging of the deep venous system from the common femoral vein to the popliteal vein was performed. Common femoral vein response to the Valsalva maneuver was recorded. Sonographically visible thrombi and abnormal vein compressibility were 91% sensitive for the common femoral vein, and 94% sensitive for the superficial femoral or popliteal veins, with no false-positive examinations. Abnormal Doppler flow and abnormal response of the common femoral vein to the Valsalva maneuver enabled thrombi to be detected only in the common femoral and iliac veins. Combined data allowed accurate diagnoses in all patients with deep venous thrombosis proximal to the deep calf veins. US should be the screening examination of choice for evaluating patients with suspected lower extremity deep venous thrombosis.
Transgenic mouse models of dopamine deficiency (2003)

The dopamine system is implicated in several neurological and psychiatric disorders. Genetic mutations or variations that affect dopamine system functions either directly cause or contribute to these disorders, even though other genetic and environmental factors may contribute significantly to some of these disorders as well. Transgenic mice increasingly become important tools in revealing functions of genes that are essential components of the dopamine system as well as in modeling human genetic disorders. We have reviewed a comprehensive list of those genes and compared genetic mutations/variations in humans and transgenic mouse models. The significance and limitations of these animal models as well as future directions are discussed.
Extracting usability information from user interface events (2000)

Modern window-based user interface systems generate user interface events as natural products of their normal operation. Because such events can be automatically captured and because they indicate user behavior with respect to an application's user interface, they have long been regarded as a potentially fruitful source of information regarding application usage and usability. However, because user interface events are typically voluminos and rich in detail, automated support is generally required to extract information at a level of abstraction that is useful to investigators interested in analyzing application usage or evaluating usability. This survey examines computer-aided techniques used by HCI practitioners and researchers to extract usability-related information from user interface events. A framework is presented to help HCI practitioners and researchers categorize and compare the approaches that have been, or might fruitfully be, applied to this problem. Because many of the techniques in the research literature have not been evaluated in practice, this survey provides a conceptual evaluation to help identify some of the relative merits and drawbacks of the various classes of approaches. Ideas for future research in this area are also presented. This survey addresses the following questions: How might user interface events be used in evaluating usability? How are user interface events related to other forms of usability data? What are the key challenges faced by investigators wishing to exploit this data? What approaches have been brought to bear on this problem and how do they compare to one another? What are some of the important open research questions in this area?
Reproduction of porcine proliferative enteropathy with pure cultures of ileal symbiont intracellularis (1993)

Porcine proliferative enteropathy is consistently associated with the presence of intracellular curved bacteria in epithelial cells in affected portions of intestine. Two strains of these intracellular bacteria were cultured in a cell culture system with rat enterocytes (IEC-18) and passaged several times and used as oral inocula for 14 gnotobiotic and 8 conventional pigs. DNA and immunological studies had identified these bacteria as belonging to a new taxon, Ileal symbiont (IS) intracellularis. Conventional pigs dosed with approximately 3.7 x 10(6) of these organisms passaged six times in cell culture developed severe lesions of proliferative enteropathy in the ileum. Other conventional pigs dosed with a lower titer or with organisms passaged 13 times developed moderate and minor lesions, respectively. All gnotobiotic pigs dosed with organisms failed to develop lesions. Control pigs, eight conventional and two gnotobiotic, dosed with diluent, uninfected cell material or left undosed failed to develop lesions also. Reisolation of IS intracellularis and demonstration of the organism in mucosal and fecal samples only occurred in conventional pigs dosed with organisms. Gnotobiotic pigs lacking a normal intestinal flora have not been shown to be colonized by the organism. Seroconversion to IS intracellularis or mucosal infiltration by inflammatory cells was not observed in experimentally affected pigs, confirming the weak immune response characteristic of the natural disease. These results support the identification of IS intracellularis as an etiological agent of proliferative enteropathy in pigs.
Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa (2005)

A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.
Toll-Like Receptor Signaling and Regulation of Cytokine Gene Expression in the Immune System (2002)

Dendritic cells, macrophages, and granulocytes are derived from hematopoietic stem cells and provide a first line of defense against infectious pathogens. Toll-like receptors (TLRs) expressed on these cells recognize molecular stuctures present in the pathogens. Upon binding of a pathogen ligand, TLRs trigger a cascade of signaling pathways that is conserved from insect to plants to humans, which ultimately activates NFkappaB. In mammalian cells, this leads to the induction of cytokine genes and the establishment of innate immunity. For example, TLR signals induce type I interferons (IFN alpha/beta) in dendritic cells conferring an antiviral state upon host cells. Moreover, TLR signals stimulate not only pro-inflammatory cytokines such as IFNs, IL-1, TNFalpha, and IL-12 but also anti-inflammatory cytokines such as IL-10 and IL-6 IL-12 and IL-10 are cytokines that bridge early innate responses and the ensuing specific immune responses. TLR signals also enhance an antigen presentation capacity in dendritic cells and macrophages. Recent studies with mouse and human cells indicate that TLRs activate multiple signaling cascades that involve chromatin structure alterations as well as activation of many transcription factors (e.g., IRF-3, IRF-8/ICSBP, and PU.1). Together, although the basic backbone is conserved throughout evolution, the TLR signaling system in mammalian species has an added complexity to accommodate a mechanism that links innate and adaptive immunity.
Substitution therapy for alcoholism: time for a reappraisal? (2011)

A number of compounds already in use as medications for various indications substitute for ethanol at clinically relevant brain pathways, in particular, at gamma-aminobutyric acid (GABA) receptors. Nevertheless, although substitute medications have been recognized for heroin and tobacco dependence, patients with alcohol dependence are rarely offered an analogous approach. Benzodiazepines may have paradoxical effects, and abuse and dependence are known. Baclofen (GABA(B) agonist) has not been associated with dependence or misuse and has been effective in several trials in preventing relapse, although research is required to establish the optimal dosing regimen. GABA-ergic anticonvulsants, helpful in treating withdrawal, have yet to emerge as effective in relapse prevention. Clomethiazole and sodium oxybate, the latter having been shown to be effective in relapse prevention, have incurred a reputation for dependence and abuse. However, data have emerged showing that the risk of abuse of sodium oxybate is lower than many clinicians had foreseen. For a condition where existing therapies are only effective in a proportion of patients, and which has high morbidity and mortality, the time now seems right for reappraising the use of substitute prescribing for alcohol dependence.
Recombinant Human Erythropoietin Stimulates Angiogenesis and Wound Healing in the Genetically Diabetic Mouse (2004)

The effects of recombinant human erythropoietin (rHuEPO) in diabetes-related healing defects were investigated by using an incisional skin-wound model produced on the back of female diabetic C57BL/KsJ-m(+/+)Lept(db) mice (db(+)/db(+)) and their normoglycemic littermates (db(+/+)m). Animals were treated with rHuEPO (400 units/kg in 100 microl s.c.) or its vehicle alone (100 microl). Mice were killed on different days (3, 6, and 12 days after skin injury) for measurement of vascular endothelial growth factor (VEGF) mRNA expression and protein synthesis, for monitoring angiogenesis by CD31 expression, and for evaluating histological changes. Furthermore, we evaluated wound-breaking strength at day 12. At day 6, rHuEPO injection in diabetic mice resulted in an increase in VEGF mRNA expression (vehicle = 0.33 +/- 0.1 relative amount of mRNA; rHuEPO = 0.9 +/- 0.09 relative amount of mRNA; P < 0.05) and protein wound content (vehicle = 23 +/- 5 pg/wound; rHuEPO = 92 +/- 12 pg/wound; P < 0.05) and caused a marked increase in CD31 gene expression (vehicle = 0.18 +/- 0.05 relative amount of mRNA; rHuEPO = 0.98 +/- 0.21 relative amount of mRNA; P < 0.05) and protein synthesis. Furthermore, rHuEPO injection improved the impaired wound healing and, at day 12, increased the wound-breaking strength in diabetic mice (vehicle = 12 +/- 2 g/mm; rHuEPO 21 +/- 5 g/mm; P < 0.05). Erythropoietin may have a potential application in diabetes-related wound disorders.