gebhart/patrepeval huggingface dataset:
Central Europe after the fall of the Iron Curtain : geopolitical perspectives, spatial patterns and trends (1998)

Contents: Francis W. Carter: Central Europe, Fact or Geographical Fiction? - Violette Rey: The New Central Europe: Waiting for Convergence? - Vladimir Kolossov: Geopolitical Scenarios for Eastern and Central Europe in a Post-bipolar World - Milan Bufon: Some Political-Geographical Problems of Transition in Central Europe: the Case of Slovenia - Mladen Klemencic: Croatia and Central Europe: Past and future Prospects - Antoni Kuklinski: Research Priorities in the Transformation of Central Europe - Petr Dostal / Martin Hampl: Transformation of East-Central Europe: General Principles under Differentiating Conditions - Gyoergy Enyedi: New Regional Processes in Post-Socialist Central Europe - Elisabeth Lichtenberger: Geography of Transition in East-Central Europe: Society and Settlement Systems - Marie-Claude Maurel: Post-Collectivist Local Societies in Central Europe - Rene Verhoeff: The Transformation of International Tourism in Central Europe - Between State and Market - Lauren Eastwood: From Revolution to Dissolution: Recent Transitions of the Eastern European Environmental Movement - Alma Bianchetti: Friuli-Venezia Giulia. Problems and future Prospects for a Central European Border and Bridge Region in the Process of European Integration - Bronislaw Kortus: Spatial-Economic Transformations in Poland - Stephan Barisitz: Changes in Trade Patterns - The Case of Austria - Marek Kupiszewski: The Future of East-West Migration in Europe - Dusan Drbohlav: The Probable Future of European East-West International Migration - Selected Aspects - Judit Juhasz / Zoltan Doevenyi: Recent International Migration Trends in Hungary. Social and Geographical Aspects - Nadine Cattan / Claude Grasland / Stanislav Rehak: Migration Flows between the Czech and Slovak Republics - Which Forms of Transition?
Structure−Activity Relationships of Polymyxin Antibiotics (2009)

ADVERTISEMENT RETURN TO ISSUEPREVPerspectiveNEXTStructure−Activity Relationships of Polymyxin AntibioticsTony Velkov*†‡, Philip E. Thompson‡, Roger L. Nation§, and Jian Li*§View Author Information† School of Medicine, Deakin University, Pigdons Road, Geelong 3217, Victoria, Australia‡ Medicinal Chemistry and Drug Action§ Facility for Anti-infective Drug Development and Innovation, Drug Delivery, Disposition and Dynamics Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville 3052, Victoria, Australia*To whom correspondence should be addressed. For T.V.: phone, +61-3-9903 9539; fax, +61-3-9903 9582; e-mail, [email protected] or [email protected]. For J.L.: phone, +61-3-9903 9702; fax, +61-3-9903 9629; e-mail, [email protected]Cite this: J. Med. Chem. 2010, 53, 5, 1898–1916Publication Date (Web):October 29, 2009Publication History Received7 July 2009Published online29 October 2009Published inissue 11 March 2010https://pubs.acs.org/doi/10.1021/jm900999hhttps://doi.org/10.1021/jm900999hreview-articleACS PublicationsCopyright © 2009 American Chemical SocietyRequest reuse permissionsArticle Views8703Altmetric-Citations568LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Acyls,Antimicrobial activity,Hydrophobicity,Lipids,Peptides and proteins Get e-Alerts
Mutation detection by solid phase primer extension (1996)

A mutation analysis method based upon a wild-type DNA sequence is presented. Oligonucleotides were utilized for primer extension by T7 DNA polymerase to discriminate between wild-type and mutant sequences in two solid phase approaches. 1. Oligonucleotides were annealed to an immobilized template, extended with fluorescent dideoxynucleotides (ddNTPs), and analyzed on an automated fluorescent DNA sequencer. The oligonucleotide length identified the known mutation site, and the fluorescence emission of the ddNTP identified the mutation. 2. Template DNA was annealed to an oligonucleotide array, extended with alpha-32P dNTPs, and analyzed with a Phosphor Imager. The grid position of the oligonucleotide identified the mutation site and the extended base identified the mutation.
T-cell lymphoma. (1985)

Of a total of 3,366 cases of malignant lymphoma in China reviewed histopathologically, T-cell lymphomas accounted for an average of 26.1% among all non-Hodgkin's lymphomas. However, the proportion of lymphomas of T-cell lineage varied in different parts of China, with the highest proportion (30-40%) along the east coast. Immunologic typing of 41 non-Hodgkin's lymphomas by the avidin-biotin-peroxidase complex technique revealed similar patterns. When serum antibodies to human T-cell leukemia virus were assayed in 462 normal men and 103 monkeys, 2-5% of the men and 8.4-15% of the monkeys were positive for antibodies to this virus.
Ecolabels: boon for the environment or confusion for the consumer? (1999)

The rise of green consumerism led to a proliferation of products claiming to be environmentally friendly, however consumers often found the statements given in support of these claims confusing. `Ecolabels&apos; are intended to help consumers recognise products which have been manufactured and which perform their intended function in an environmentally friendly way. This article gives an overview of the current position regarding ecolabelling within the Member States of the European Union (EU) and proposals for strengthening the EU Ecolable Regulations.
High reflectivity fibre gratings produced by incubateddamage using a 193 nm ArF laser (1994)

High reflectivity gratings have been formed in optical fibre using a 193 nm ArF laser irradiated phase mask. Relatively high absorption in the fibre core at this wavelength coupled with an incubation effect in which the absorption grows with repeated exposure allows rapid grating formation (~10 pulses) at modest fluences (~400 m Jcm-2).
ChemInform Abstract: HETERODIENE SYNTHESES. XXIV. CHANGES IN THE REACTIVITY OF 2‐OXOINDOLIN‐3‐YLIDENE DERIVATIVES WITH ETHYL VINYL ETHER INDUCED BY ELECTRON‐WITHDRAWING GROUPS. THE IMPORTANCE OF THE LUMO COEFFICIENTS (1981)

Abstract Die Umsetzung des Vinylethers (II) mit den Oxoindolinyliden‐Derivaten (I) bei 100°C liefert die Produkte (III), (IV), (V), (VI) + (VII) bzw. (VIIIa)+(VIIIb) +(IXa) + (IXb).
Characterization of YafO, an Escherichia coli Toxin (2009)

YafO is a toxin encoded by the yafN-yafO antitoxin-toxin operon in the Escherichia coli genome. Our results show that YafO inhibits protein synthesis but not DNA or RNA synthesis. The in vivo [35S]methionine incorporation was inhibited within 5 min after YafO induction. In in vivo primer extension experiments with two different mRNAs, the specific cleavage bands appeared 11-13 bases downstream of the initiation codon, AUG, 2.5 min after the induction of YafO. An identical band was also detected in in vitro toeprinting experiments when YafO was added to the reaction mixture containing 70 S ribosomes and the same mRNAs even in the absence of tRNA(f)(Met). Notably, this band was not detected in the presence of YafO alone, indicating that YafO by itself does not have endoribonuclease activity under the conditions used. The full-length mRNAs almost completely disappeared 30 min after YafO induction in in vivo primer extension experiments, consistent with Northern blotting analysis. Over 84% of [35S]methionine-tRNA(f)(Met) was released from the translation initiation complex at 5.43 microM YafO in vitro. We demonstrated that the 70 S ribosome peak significantly increased upon YafO induction, and when the 70 S ribosomes dissociated into 50 and 30 S subunits, YafO was found to be associated with 50 S subunits. These results demonstrate that YafO is a ribosome-dependent mRNA interferase inhibiting protein synthesis.
Constitutive Expression of Human Intercellular Adhesion Molecule-1 (ICAM-1) is Regulated by Differentially Active Enhancing and Silencing Elements (1995)

While expression of intracellular adhesion molecule 1 (ICAM-1; CD54) is associated with chronic inflammation and autoimmune disease, and is also found on some tumours arising from ICAM-1-negative tissues, in apparently normal tissues it is restricted to a few cell types. Levels of constitutive ICAM-1 expression correlate with the levels of ICAM-1 mRNA. In order to identify regions of the gene regulating its constitutive expression, 5.8 kb of the 5' upstream region was studied in 16 human cell lines using transient transfection of reporter-gene constructs. Three enhancing and one silencing region were observed. While the enhancer upstream of position -1352 was active in all cells investigated, the inhibitory influence of a silencer region between positions -339 and -290 was observed only in 50% of the cells. All cells expressing low levels of ICAM-1, such as may occur in many tissues in vivo, lacked an active silencer. In contrast to the ICAM-1 low-expressing cells, cell lines with high constitutive ICAM-1 levels, as well as those with no ICAM-1 expression, showed an active silencer. Thus, ICAM-1 constitutive expression seems to be regulated in two different ways. The fact that silencer and enhancer activities were observed in both strongly positive and negative ICAM-1 cells, suggests that constitutive ICAM-1 expression is regulated by a balance of enhancing and silencing transcription factors and possible additional elements.
A Controlled Study of Drugs in Long-term Geriatric Psychiatric Patients (1971)

At dosages of up to 600 mg of pentylenetetrazol (Metrazol), 450 mg of papaverine, and 45 mg of niacin per day, over a three-month period, only very small clinical changes were observed in 60 long-term psychotic geriatric patients randomly assigned to the three treatments. If one assumes niacin to be an active placebo, one finds that pentylenetetrazol elicits significantly more clinical improvement than placebo. This effect was modest and was not shown on other measures. Papaverine increased hallucinations relative to placebo. pentylenetetrazol increased motor retardation relative to placebo and improved hostility relative to papaverine. None of the treatments showed any significant differential effect in reducing intellectual impairment. Measures used included the BPRS, the NOSIE, and two scales, the Mental Status and Activities of Daily Living scales developed by Stotsky for use in studies of geriatric patients.
Continuous subcutaneous infusion of glucagon-like peptide 1 lowers plasma glucose and reduces appetite in type 2 diabetic patients. (1999)

We conclude that 48-h continuous subcutaneous infusion of GLP-1 in type 2 diabetic patients 1) lowers fasting as well as meal-related plasma glucose, 2) reduces appetite, 3) has no gastrointestinal side effects, and 4) has no negative effect on blood pressure.
Opposing Effect of EGFRWT on EGFRvIII-Mediated NF-κB Activation with RIP1 as a Cell Death Switch (2013)

RIP1 is a central mediator of cell death in response to cell stress but can also mediate cell survival by activating NF-κB. Here, we show that RIP1 acts as a switch in EGFR signaling. EGFRvIII is an oncogenic mutant that does not bind ligand and is coexpressed with EGFRWT in glioblastoma multiforme (GBM). EGFRvIII recruits ubiquitin ligases to RIP1, resulting in K63-linked ubiquitination of RIP1. RIP1 binds to TAK1 and NEMO, forming an EGFRvIII-RIP1 signalosome that activates NF-κB. RIP1 is essential for EGFRvIII-mediated oncogenicity and correlates with NF-κB activation in GBM. Surprisingly, activation of EGFRWT with EGF results in a negative regulation of EGFRvIII, with dissociation of the EGFRvIII-RIP1 signalosome, loss of RIP1 ubiquitination and NF-κB activation, and association of RIP1 with FADD and caspase-8. If EGFRWT is overexpressed with EGFRvIII, the addition of EGF leads to a RIP1 kinase-dependent cell death. The EGFRWT-EGFRvIII-RIP1 interplay may regulate oncogenicity and vulnerability to targeted treatment in GBM.
Urethane-protected α-amino acid N-carboxyanhydrides and peptide synthesis (1996)

The preparation, physical properties and analytical data are reported for seventy urethane-protected (Boc, Cbz, FMOC) amino acid N-carboxyanhydrides (UNCAs). Most of the UNCAs are crystalline and the X-ray diffraction patterns for several of these are described. UNCAs are stable to routine laboratory manipulations and can be stored for extended periods of time (1-2 years at below 0 degrees C). Most are completely stable to the conditions commonly employed for peptide synthesis. The correct choice of base is key for the successful introduction of urethane protecting groups into NCAs. N-Methylmorpholine is used for the introduction of FMOC, Cbz or Boc from the chloroformates, and pyridine is used for the introduction of the Boc group from Boc anhydride. UNCAs represent a unique class of preactivated, isolable and stable amino acid derivatives that generate no side products or co-products, other than CO2, during condensation reactions. The application of UNCAs in peptide synthesis in both solid phase and in solution is reviewed in detail.
Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line. (1996)

Adeno-associated virus-2 (AAV) can integrate in a site-specific manner to human chromosome 19 and is currently in phase I clinical trials for cystic fibrosis (CF) at Johns Hopkins Hospital. The goal of this study was to determine the fate of recombinant AAV containing the CFTR cDNA (AAV-CFTR) in an immortalized pseudotetraploid CF bronchial epithelial cell line (IB3-1) established from a patient with CF. Fluorescence in situ hybridization (FISH) and Southern blotting of DNA from IB3-1 cells infected with wild-type (wt) or recombinant AAV-CFTR were performed. CFRH2, an IB3-1 cell line with an estimated 15-20 integrated copies of CFTR cDNA, was used to test FISH sensitivity. All metaphase spreads had integrated copies: a single site in 36 of 56 (64.3%) and two sites within the same metaphase spread in 20 of 56 (35.7%). 3-CF-8, an IB3-1 cell line with integration of a partial CFTR cDNA (3.9 kb) was also analyzed by FISH. Integration was observed in 56 of 157 (35.7%) metaphase spreads examined. IB3-1 cells infected with wild-type AAV showed integration in 51 of 86 (59%) metaphase spreads examined. Of 51 integrations, 48 (94%) were to chromosome 19. Examination of 67 metaphase chromosome spreads of IB3-1 cells infected with AAV-CFTR vector (Azero) identified four integrations (6%) to different chromosomes. No integration was to chromosome 19 which differs significantly (P < 0.0001) from wild-type AAV. We then analyzed the A35 cell line, a clone of Azero selected for stable CFTR expression. Genomic DNA from A35 cells did not show a single site of integration; however episomal AAV-CFTR sequences were abundant in the low molecular weight DNA fraction. Examination of 68 metaphase chromosome preparations identified eight distinct integrations, none to chromosome 19. These studies show that FISH is sensitive for the detection of a partial CFTR cDNA integration. Wild-type AAV integrates in a predominantly site-specific fashion. Recombinant AAV-CFTR integrates at low frequency in a nonspecific manner and persists in episomal form in this epithelial cell line.
A higher order topology for interpolative modulators for oversampling A/D converters (1990)

Oversampling interpolative coding has been demonstrated to be an effective technique for high-resolution analog-to-digital (A/D) conversion that is tolerant of process imperfections. A novel topology for constructing stable interpolative modulators of arbitrary order is described. Analysis of this topology shows that with proper design of the modulator coefficients, stability is not a limitation to higher order modulators. Furthermore, complete control over placement of the poles and zeros of the quantization noise response allows treatment of the modulation process as a high-pass filter for quantization noise. Higher order modulators are shown not only to greatly reduce oversampling requirements for high-resolution conversion applications, but also to randomize the quantization noise, avoiding the need for dithering. An experimental fourth-order modulator breadboard demonstrates stability and feasibility, achieving a 90-dB dynamic range over the 20-kHz audio bandwidth with a sampling rate of 2.1 MHz. A generalized simulation software package has been developed to mimic time-domain behavior for oversampling modulators. Circuit design specifications for integrated circuit implementation can be deduced from analysis of simulated data.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
IL-10 Regulates Murine Lupus (2002)

MRL/MpJ-Tnfrsf6(lpr) (MRL/MpJ-Fas(lpr); MRL-Fas(lpr)) mice develop a spontaneous lupus syndrome closely resembling human systemic lupus erythematosus. To define the role of IL-10 in the regulation of murine lupus, IL-10 gene-deficient (IL-10(-/-)) MRL-Fas(lpr) (MRL-Fas(lpr) IL-10(-/-)) mice were generated and their disease phenotype was compared with littermates with one or two copies of an intact IL-10 locus (MRL-Fas(lpr) IL-10(+/-) and MRL-Fas(lpr) IL-10(+/+) mice, respectively). MRL-Fas(lpr) IL-10(-/-) mice developed severe lupus, with earlier appearance of skin lesions, increased lymphadenopathy, more severe glomerulonephritis, and higher mortality than their IL-10-intact littermate controls. The increased severity of lupus in MRL-Fas(lpr) IL-10(-/-) mice was closely associated with enhanced IFN-gamma production by both CD4(+) and CD8(+) cells and increased serum concentration of IgG2a anti-dsDNA autoantibodies. The protective effect of IL-10 in this lupus model was further supported by the observation that administration of rIL-10 reduced IgG2a anti-dsDNA autoantibody production in wild-type MRL-Fas(lpr) animals. In summary, our results provide evidence that IL-10 can down-modulate murine lupus through inhibition of pathogenic Th1 cytokine responses. Modulation of the level of IL-10 may be of potential therapeutic benefit for human lupus.
Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus (1990)

The lentivirus caprine arthritis-encephalitis virus (CAEV) is closely related by nucleotide sequence homology to visna virus and other sheep lentiviruses and shows less similarity to the other animal and human lentiviruses. The genomic organization of CAEV is very similar to that of visna virus and the South African ovine maedi visna virus (SA-OMVV) as well as to those of other primate lentiviruses. The CAEV genome includes the small open reading frames (ORF) between pol and env which are the hallmarks of the lentivirus genomes. The most striking difference in the organization of CAEV is in the env gene. The Env polyproteins of visna virus and the related SA-OMVV contain 20 amino acids between the translational start and the signal peptide not present in CAEV. In addition to nucleotide sequence analysis, the transcriptional products of CAEV were determined by Northern analysis. The viral mRNA present in cells transfected with the infectious clone reveal a pattern characteristic of the mRNAs observed in other lentivirus infections. The putative tat ORF of CAEV could be identified by genomic location and amino acid homology to the visna virus tat gene. However, the CAEV rev gene could not be identified in a similar fashion. Thus, to determine the location of the rev ORF cDNA clones were obtained by PCR amplification of the mRNA from infected cells. To determine if a Rev response element was contained in the CAEV genome, secondary structural analysis of the viral RNA was performed. A stable stem loop structure which is similar in location, stability, and configuration to that determined for the Rev response element of HIV was found.
Roles of acetate and phosphate in the successful storage of platelet concentrates prepared with an acetate‐containing additive solution (1993)

The development of a synthetic medium for platelet storage is an important goal in transfusion medicine. Its use would make large volumes of plasma available for fractionation and might improve the quality of platelets after storage. Several investigators have described successful storage in media containing acetate. The previous work of the authors showed that platelet concentrates (PCs) can be stored successfully for 5 days at 22 degrees C by using an additive solution (Seto sol) to replace 80 to 95 percent of the plasma usually employed as a suspending medium. Seto sol contains 23 mM (23 mmol/L) sodium acetate and 25 mM (25 mmol/L) sodium phosphate. The roles of acetate and phosphate in achieving successful platelet storage were studied in the work reported here. The concentration of acetate decreased linearly for 7 days at 0.61 +/- 0.11 mumol per day per 10(9) platelets in parallel with the disappearance of 1-14C or 2-14C acetate. There was no disappearance of tritiated acetate from PCs or of 1-14C acetate from platelet-free mixtures of plasma and Seto sol, which suggests that the disappearance of 14C acetate from PCs reflected oxidation to CO2, which could leave PCs through the walls of the plastic container. Since O2 consumption was 1.47 mumol per day per 10(9) platelets, and the oxidation of a molecule of acetate requires 2 molecules of oxygen, acetate oxidation accounted for approximately 85 percent of oxygen consumption by platelets. The pH of PCs stored in Seto sol was nearly constant for 7 days, whereas, without acetate, it fell to 6.4 +/- 0.1 on Day 5.(ABSTRACT TRUNCATED AT 250 WORDS)
Interaction of Daptomycin with Lipid Bilayers: A Lipid Extracting Effect (2014)

Daptomycin is the first approved member of a new structural class of antibiotics, the cyclic lipopeptides. The peptide interacts with the lipid matrix of cell membranes, inducing permeability of the membrane to ions, but its molecular mechanism has been a puzzle. Unlike the ubiquitous membrane-acting host-defense antimicrobial peptides, daptomycin does not induce pores in the cell membranes. Thus, how it affects the permeability of a membrane to ions is not clear. We studied its interaction with giant unilamellar vesicles (GUVs) and discovered a lipid-extracting phenomenon that correlates with the direct action of daptomycin on bacterial membranes observed in a recent fluorescence microscopy study. Lipid extraction occurred only when the GUV lipid composition included phosphatidylglycerol and in the presence of Ca(2+) ions, the same condition found to be necessary for daptomycin to be effective against bacteria. Furthermore, it occurred only when the peptide/lipid ratio exceeded a threshold value, which could be the basis of the minimal inhibitory concentration of daptomycin. In this first publication on the lipid extracting effect, we characterize its dependence on ions and lipid compositions. We also discuss possibilities for connecting the lipid extracting effect to the antibacterial activity of daptomycin.