gebhart/patrepeval huggingface dataset:
Effects of low-volume hemoglobin glutamer-200 versus normal saline and arginine vasopressin resuscitation on systemic and skeletal muscle blood flow and oxygenation in a canine hemorrhagic shock model* (2007)

Low-volume crystalloid or hemoglobin glutamer-200 resuscitation posthemorrhage may improve (but not restore) macro- and microvascular functions and tissue oxygenation, while arginine vasopressin infusion may only improve blood pressures and result in lower overall systemic perfusion compared with low-volume saline or hemoglobin glutamer-200 treatment and worsening of anaerobic conditions in skeletal muscle.
Naked DNA vaccines expressing the prM and E genes of Russian spring summer encephalitis virus and Central European encephalitis virus protect mice from homologous and heterologous challenge (1997)

Naked DNA vaccines expressing the prM and E genes of two tick-borne flaviviruses, Russian spring summer encephalitis (RSSE) virus and Central European encephalitis (CEE) virus were evaluated in mice. The vaccines were administered by particle bombardment of DNA-coated gold beads by Accell gene gun inoculation. Two immunizations of 0.5 to 1 microg of RSSE or CEE constructs/dose, delivered at 4-week intervals, elicited cross-reactive antibodies detectable by enzyme-linked immunosorbent assay and high-titer neutralizing antibodies to CEE virus. Cross-challenge experiments demonstrated that either vaccine induced protective immunity to homologous or heterologous RSSE or CEE virus challenge. The absence of antibody titer increases after challenge and the presence of antibodies to E and prM, but not NS1, both before and after challenge suggest that the vaccines prevented productive replication of the challenge virus. One vaccination with 0.5 microg of CEE virus DNA provided protective immunity for at least 2 months, and two vaccinations protected mice from challenge with CEE virus for at least 6 months.
Pulse energy gain saturation in subpico- and picosecond pulse amplification by a traveling-wave semiconductor laser amplifier (1989)

Optical pulses with durations ranging from 0.49 to 21 ps are amplified by a traveling-wave semiconductor laser amplifier. The pulse energy gain is determined by pulse energy only. The dependence of pulse energy gain on output pulse energy does not change in the pulse duration range. The saturation characteristics are successfully explained by a four-level system model.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Differential expression of Rb2/p130 and p107 in normal human tissues and in primary lung cancer. (1997)

Two proteins, p107 and pRb2/p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. Using immunocytochemical techniques, we examined a variety of normal human tissues for the expression of pRb2/p130 and p107. Both proteins were expressed ubiquitously, although a different tissue distribution and/or level of expression were found in various organs. Terminally differentiated cells, such as neurons and skeletal muscle, showed high expression levels for Rb2/p130, whereas p107 was expressed at higher levels in other cell types such as epithelia of the breast and prostate. We then examined the expression pattern of Rb2/p130 in 158 specimens of human lung cancer and found an inverse correlation between the histological grading of the tumors, the development of metastasis, and the level of expression of Rb2/p130.
Effect of a single ethanol exposure on HIV replication in human lymphocytes. (1997)

Our results are consistent with either a subtle, study-induced stress-related enhancement in HIV replication or significant individual variation in response to ethanol. The results do not provide evidence for a general increase in HIV replication in the lymphocytes of subjects following a single in vivo ethanol dose of 1 g/kg.
Biodehalogenation. Reductive reactivities of microbial and mammalian cytochromes P-450 compared with heme and whole-cell models (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiodehalogenation. Reductive reactivities of microbial and mammalian cytochromes P-450 compared with heme and whole-cell modelsCharles E. Castro, Wallace H. Yokoyama, and Nao O. BelserCite this: J. Agric. Food Chem. 1988, 36, 5, 915–919Publication Date (Print):September 1, 1988Publication History Published online1 May 2002Published inissue 1 September 1988https://pubs.acs.org/doi/10.1021/jf00083a007https://doi.org/10.1021/jf00083a007research-articleACS PublicationsRequest reuse permissionsArticle Views54Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cell types and morphogenesis in the mammary gland. (1982)

Cell types present in the mammary gland and their evolution were studied by labeling female rats with radioactive thymidine at various phases of the estrus cycle. The results suggest that the stem cells for mammary development are present in the terminal end buds and that they generate a lineage for lumenal cells and possibly a distinct one for myoepithelial cells. Growth and differentiation are controlled by both hormones and local factors.
Proline‐Catalyzed Asymmetric Aldol Reaction Between Methyl Ketones and 1‐Aryl‐2,2,2‐trifluoroethanones. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Thymidine Phosphorylase Gene Mutations in MNGIE, a Human Mitochondrial Disorder (1999)

Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an autosomal recessive human disease associated with multiple deletions of skeletal muscle mitochondrial DNA (mtDNA), which have been ascribed to a defect in communication between the nuclear and mitochondrial genomes. Examination of 12 MNGIE probands revealed homozygous or compound-heterozygous mutations in the gene specifying thymidine phosphorylase (TP), located on chromosome 22q13.32-qter. TP activity in leukocytes from MNGIE patients was less than 5 percent of controls, indicating that loss-of-function mutations in TP cause the disease. The pathogenic mechanism may be related to aberrant thymidine metabolism, leading to impaired replication or maintenance of mtDNA, or both.
The Carboxyl Terminus of Neph Family Members Binds to the PDZ Domain Protein Zonula Occludens-1 (2003)

The PSD95/Dlg/ZO-1 (PDZ) domain-containing protein zonula occludens-1 (ZO-1) selectively localizes to the cytoplasmic basis of the slit diaphragm, a specialized cell-cell contact in between glomerular podocytes necessary to prevent the loss of protein in the urine. However, the function of ZO-1 at the slit diaphragm has remained elusive. Deletion of Neph1, a slit diaphragm protein of the immunoglobulin superfamily with a cytoplasmic PDZ binding site, causes proteinuria in mice. We demonstrate now that Neph1 binds ZO-1. This interaction was mediated by the first PDZ domain of ZO-1 and involved the conserved PDZ domain binding motif present in the carboxyl terminus of the three known Neph family members. Furthermore, Neph1 co-immunoprecipitates with ZO-1 from lysates of mouse kidneys, demonstrating that this interaction occurs in vivo. Both deletion of the PDZ binding motif of Neph1 as well as threonine-to-glutamate mutation of the threonine within the binding motif abrogated binding of ZO-1, suggesting that phosphorylation may regulate this interaction. ZO-1 binding was associated with a strong increase in tyrosine phosphorylation of the cytoplasmic tail of Neph1 and dramatically accelerated the ability of Neph1 to induce signal transduction. Thus, our data suggest that ZO-1 may organize Neph proteins and recruit signal transduction components to the slit diaphragm of podocytes.
Paclitaxel-Induced Nuclear Translocation of FOXO3a in Breast Cancer Cells Is Mediated by c-Jun NH2-Terminal Kinase and Akt (2006)

The microtubule-targeting compound paclitaxel is often used in the treatment of endocrine-resistant or metastatic breast cancer. We have previously shown that apoptosis of breast cancer cells in response to paclitaxel is mediated by induction of FOXO3a expression, a transcription factor downstream of the phosphatidylinositol-3-kinase/Akt signaling pathway. To further investigate its mechanism of action, we treated MCF-7 cells with paclitaxel and showed a dose-dependent increase in nuclear localization of FOXO3a, which coincided with decreased Akt signaling but increased c-Jun NH2-terminal kinase 1/2 (JNK1/2), p38, and extracellular signal-regulated kinase 1/2 (ERK1/2) activity. Flow cytometry revealed that paclitaxel-induced apoptosis of MCF-7 cells and of other paclitaxel-sensitive breast cancer cell lines was maintained in the presence of inhibitors of p38 (SB203580) or mitogen-activated protein/ERK kinase 1 signaling (PD98059) but abrogated when cells were treated with the JNK1/2 inhibitor SP600125. SP600125 reversed Akt inhibition and abolished FOXO3a nuclear accumulation in response to paclitaxel. Moreover, conditional activation of JNK mimicked paclitaxel activity and led to dephosphorylation of Akt and FOXO3a. Furthermore, mouse embryonic fibroblasts (MEF) derived from JNK1/2 knockout mice displayed very high levels of active Akt, and in contrast to wild-type MEFs, paclitaxel treatment did not alter Akt activity or elicit FOXO3a nuclear translocation. Taken together, the data show that cell death of breast cancer cells in response to paclitaxel is dependent upon JNK activation, resulting in Akt inhibition and increased FOXO3a activity.
Technology in the Employment Interview: A Meta-Analysis and Future Research Agenda (2016)

The use of technology such as telephone and video has become common when conducting employment interviews. However, little is known about how technology affects applicant reactions and interviewer ratings. We conducted meta-analyses of twelve studies that resulted in K=13 unique samples and N=1,557. Mean effect sizes for interview medium on ratings (d=-.41) and reactions (d=-.36) were moderate and negative, suggesting that interviewer ratings and applicant reactions are lower in technology-mediated interviews. Generalizing research findings from face-to-face interviews to technology-mediated interviews is inappropriate. Organizations should be especially wary of varying interview mode across applicants, as inconsistency in administration could lead to fairness issues. At the same time, given the limited research that exists, we call for renewed attention and further studies on potential moderators of this effect.
Ras Oncogene Mutations in Thyroid Tumors (1996)

Ras mutations have been found in thyroid lesions. Different studies have shown different frequencies of mutations among benign and malignant lesions. The presence of point mutations in codons 12 and 13 of the c-K-ras, c-H-ras, and N-ras genes was studied in 58 thyroid lesions (10 nodular goiters, 10 follicular adenomas, and 15 papillary, 10 follicular, and 13 anaplastic carcinomas). DNA was extracted from formalin-fixed paraffin-embedded tissue, and target sequences were amplified in vitro by the polymerase chain reaction. Mutations were detected by the presence of restriction-fragment-length polymorphisms either occurring naturally or introduced artificially by the use of mutant primers. No characterization of the mutations was performed. Results were correlated with clinicopathologic features and patient follow-up. One goiter showed a mutation at codon 13, c-K-ras. All follicular adenomas, including three hyalinizing trabecular adenomas, were negative. Four papillary carcinomas presented mutations (one at codon 13, c-K-ras; three at codon 12, N-ras). Two follicular carcinomas showed mutations at codon 12, N-ras. Five anaplastic carcinomas showed mutations (two at codon 12 and two at codon 13, c-K-ras; one at codon 12, N-ras). In summary, the results confirm that ras oncogenes play a role in thyroid tumorigenesis, probably at an early step. Ras mutations appear not to be related to prognosis.
Complete Tumor Ablation With Iodine 131-Radiolabeled Disialoganglioside GD2-Specific Monoclonal Antibody Against Human Neuroblastoma Xenografted in Nude Mice23 (1986)

The antibody 3F8, an IgG3 murine monoclonal antibody (MoAb) against disialoganglioside GD2, could target iodine-131 (131I) to established subcutaneous human neuroblastoma (NB) xenografts in BALB/c nude mice. 131I-radiolabeled MoAb (0.125-1 mCi) was injected iv. Tumor radioactivity over time was calculated from scintigraphy, and radiation dose to individual tumors was calculated. Tumor shrinkage occurred only with 131I-labeled 3F8, but not with nonradioactive 3F8 or radiolabeled irrelevant antibody. While the tumor of the control mice enlarged by tenfold, the treated tumor showed over 95% shrinkage by 12 days. Both the rate of shrinkage and duration of tumor response were dose dependent. Calculated doses of more than 10,000 rad could be achieved. Only those tumors that received more than 4,200 rad were completely ablated without recurrence. Recurrent tumors were not antigen negative or radioresistant. These results confirmed the prediction based on imaging studies that human NB xenografts could be effectively eradicated with the use of 131I-labeled MoAb 3F8 with tolerable toxicities.
An early evaluation of malignant tendency with plectin expression in human colorectal adenoma and adenocarcinoma. (2004)

Tumor cells are morphologically different from normal cells. In this situation, we proposed that plectin, one of the intermediate filament associated proteins, might play some special roles in the tumorigenesis. Plectin exhibits a wide distribution spectrum among various tissues; however, it is scarcely investigated in tumor tissues including colorectal adenocarcinoma. In this study, we searched the plectin expression in 25 cases of colorectal adenocarcinoma and 10 cases of tubular adenoma with focal adenocarcinoma by immunohistochemical method. The results reveal that plectin is up-regulated in colorectal adenocarcinoma as well as in bizarre glands and locally invasive tumor nests in tubular adenoma, compared with normal colorectal mucosa. Over-expression of plectin in locally invasive tumor nests might help us in diagnosis of distinguish microinvasive foci from normal glands. The bizarre glands in adenoma might be precancerous lesions since the expression of plectin in them was identical to that of adenocarcinoma but in contrast to that of normal glands. The overexpression of plectin might affect the organization of cytoskeleton, which might further cause tumorigenesis and morphological change of colorectal epithelial cells.
Schizophrenia: Neural Mechanisms for Novel Therapies (2003)

Although valuable antischizophrenic drugs exist, they only partially ameliorate symptoms and elicit substantial side effects. Classic neuroleptic drugs act by blocking dopamine receptors. They can relieve some symptoms but not behavioral withdrawal features that are designated "negative" symptoms. Clozapine and related newer atypical neuroleptics may be more efficacious in relieving negative symptoms. Understandng their actions may facilitate new drug discovery. Agents influencing glutamate neurotransmission and N-methyl-D-aspartate receptors, especially the cotransmitter D-serine, are promising. Stimulation of the alpha7 subtype of nicotinic acetylcholine receptor may also be efficacious. The search for genes linked to schizophrenia has revealed several leads that may permit development of novel therapeutic agents. Promising genes include disrupted-in-schizophrenia-1, dysbindin, and neuregulin.
The Effects of Fuel Properties and Oxygenates on Diesel Exhaust Emissions (1995)

&lt;div class="htmlview paragraph"&gt;The effects of diesel fuel properties (aromatic content, cetane index and T90), cetane improver, oxygenates, high boiling point hydrocarbons and aromatics distribution on diesel exhaust emissions were studied under the Japanese 10-15 test cycle and the ECE+EUDC test cycle. The test vehicle was a TOYOTA COROLLA with a natural aspirated, 2.0L displacement, IDI diesel engine.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;It was demonstrated that particulate emissions are highly correlated with T90 and that NOx is affected by the aromatic content of fuel. A reduction in particulates emissions was observed in fuel with a lower cetane number by adding cetane improver, but this reduction was limited. Cetane improver had no effect on NOx emissions in the 45 # 60 cetane number range. Oxygenates reduced particulate emissions remarkably but had little effect on NOx emissions. A decrease in the soot in particulates was particularly observed. Particulate emissions seemed to be more affected by the blended oxygen content of oxygenated fuel than by its molecular structure, cetane number or boiling point. The reduction in particulates with oxygenated fuels was larger under the ECE+EUDC test cycle than the Japanese 10-15 test cycle. Di- and Triaromatics affected PM emissions more than Paraffin or Mono-aromatics.&lt;/div&gt; &lt;div class="htmlview paragraph"&gt;Furthermore, the effects of our test fuels were evaluated using a DI Diesel engine. Fuel with lower T90 and lower aromatic content reduced PM by 40% in the Japanese diesel 13 mode test. Cetane improver reduced ignition delay effectively, but had no effect on PM or NOx. Oxygenated fuel reduced PM emissions, however oxygenated fuel with a low cetane number increased PM given a specific injection timing under a high load condition.&lt;/div&gt;
Establishment of the mouse embryonic stem cell lines from whole blastocysts and isolated inner cell masses. (1989)

129/SVJ系マウスより得た,3.5日齢の胚盤胞あるいは免疫手術法により単離した内部細胞塊を,マイトマイシンC処理胎子線維芽細胞(C57BL由来)層上で継代培養を行った。その結果,異なる胚盤胞に由来する6株(7%,6/85;TT-3,-12,-23,-B2,-B3,-B4)の胚性幹細胞株を得た。これらの細胞株は,胚性癌細胞に類似した細胞境界が不明瞭でコンパクトな島状のコロニーを形成した。2株(TT-12,TT-B4)について染色体分析を行ったところ,2株とも観察した分裂中期像の78%(39/50)が正常2倍体の核型を有していた。細胞株の分化能を調べるために,増殖性の良好な細胞株(TT-12)を浮遊条件で培養した。その結果,外層が内胚葉と思われる組織に分化し,内部に外胚葉および前羊膜腔に類似した組織の形成が認められる胚様体が形成され,in vitroにおいて多能性が認められた。
A soluble divalent class I major histocompatibility complex molecule inhibits alloreactive T cells at nanomolar concentrations. (1993)

Genetically engineered or chemically purified soluble monovalent major histocompatibility complex (MHC) molecules, which have previously been used to study T cells, have not blocked cytotoxic T-cell responses. Here we describe a genetically engineered divalent class I MHC molecule which inhibits lysis of target cells by alloreactive cytotoxic T cells. This protein, H-2Kb/IgG, was generated as a fusion protein between the extracellular domains of a murine class I polypeptide, H-2Kb, and an immunoglobulin heavy chain polypeptide. The chimeric protein has serological and biochemical characteristics of both the MHC and IgG polypeptides. Nanomolar concentrations of H-2Kb/IgG inhibited lysis of H-2Kb-expressing target cells not only by alloreactive H-2Kb-specific T-cell clones but also by alloreactive H-2Kb-specific primary T-cell cultures. A direct binding assay showed high-affinity binding between the H-2Kb/IgG molecule and an H-2Kb-specific alloreactive T-cell clone. Unlabeled H-2Kb/IgG displaced 125I-labeled H-2Kb/IgG from T cells with an IC50 of 1.2 nM.
HeadGesture (2018)

We propose HeadGesture, a hands-free input approach to interact with Head Mounted Display (HMD) devices. Using HeadGesture, users do not need to raise their arms to perform gestures or operate remote controllers in the air. Instead, they perform simple gestures with head movement to interact with the devices. In this way, users' hands are free to perform other tasks, e.g., taking notes or manipulating tools. This approach also reduces the hand occlusion of the field of view [11] and alleviates arm fatigue [7]. However, one main challenge for HeadGesture is to distinguish the defined gestures from unintentional movements. To generate intuitive gestures and address the issue of gesture recognition, we proceed through a process of Exploration - Design - Implementation - Evaluation. We first design the gesture set through experiments on gesture space exploration and gesture elicitation with users. Then, we implement algorithms to recognize the gestures, including gesture segmentation, data reformation and unification, feature extraction, and machine learning based classification. Finally, we evaluate user performance of HeadGesture in the target selection experiment and application tests. The results demonstrate that the performance of HeadGesture is comparable to mid-air hand gestures, measured by completion time. Additionally, users feel significantly less fatigue than when using hand gestures and can learn and remember the gestures easily. Based on these findings, we expect HeadGesture to be an efficient supplementary input approach for HMD devices.
Tumor-Infiltrating Lymphocytes Expressing the Tissue Resident Memory Marker CD103 Are Associated with Increased Survival in High-Grade Serous Ovarian Cancer (2013)

CD103(+) TILs comprise intraepithelial, activated CD8(+) T cells, and NK cells and are strongly associated with patient survival in HGSC. CD103 may serve as a useful marker for enriching the most beneficial subsets of TILs for immunotherapy.
Membrane Association of the CD3ε Signaling Domain Is Required for Optimal T Cell Development and Function (2014)

The TCR:CD3 complex transduces signals that are critical for optimal T cell development and adaptive immunity. In resting T cells, the CD3ε cytoplasmic tail associates with the plasma membrane via a proximal basic-rich stretch (BRS). In this study, we show that mice lacking a functional CD3ε-BRS exhibited substantial reductions in thymic cellularity and limited CD4- CD8- double-negative (DN) 3 to DN4 thymocyte transition, because of enhanced DN4 TCR signaling resulting in increased cell death and TCR downregulation in all subsequent populations. Furthermore, positive, but not negative, T cell selection was affected in mice lacking a functional CD3ε-BRS, which led to limited peripheral T cell function and substantially reduced responsiveness to influenza infection. Collectively, these results indicate that membrane association of the CD3ε signaling domain is required for optimal thymocyte development and peripheral T cell function.