gebhart/patrepeval huggingface dataset:
A Drosophila Homolog of the Yeast Origin Recognition Complex (1995)

Genes from Drosophila melanogaster have been identified that encode proteins homologous to Orc2p and Orc5p of the Saccharomyces cerevisiae origin recognition complex (ORC). The abundance of the Drosophila Orc2p homolog DmORC2 is developmentally regulated and is greatest during the earliest stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts revealed that DmORC2 is found in a tightly associated complex with five additional polypeptides, much like the yeast ORC. These studies will enable direct testing of the initiator-based model of replication in a metazoan.
Fibroblast growth factor 4 transfection of MCF-7 cells produces cell lines that are tumorigenic and metastatic in ovariectomized or tamoxifen-treated athymic nude mice. (1993)

Successful antiestrogen treatment in patients with tamoxifen-responsive breast tumors is often followed by an outgrowth of tumors cells that are antiestrogen resistant, implying that estrogen-dependent tumors can become estrogen-independent. In an effect to mimic this progression, we have transfected fibroblast growth factor 4 into MCF-7 cells, a human breast carcinoma cell line that is estrogen-dependent for growth in nude mice. This transfection results in cell lines that form progressively growing, metastatic tumors when injected s.c. into untreated or tamoxifen-treated ovariectomized nude mice. In contrast to the parental cell line, growth of transfected cells in ovariectomized nude mice is stimulated by tamoxifen treatment and inhibited by estrogen treatment of the mice. Parental MCF-7 cells were transfected with an expression vector for beta-galactosidase, conferring the ability to convert the chromogenic substrate, 5-bromo-4-chloro-3-indoyl-beta-galactoside, to a blue color and allowing the detection of their presence within tumors developing after coinoculation with fibroblast growth factor 4-transfected cells. The fibroblast growth factor 4-transfected cells could support growth and metastasis of the beta-galactosidase-expressing parental cell line when both lines were coinjected into the same site in untreated or tamoxifen-treated, ovariectomized mice. These data suggest a possible role for fibroblast growth factors in the progression of breast tumors to an estrogen-independent, antiestrogen-resistant, metastatic phenotype. They also support a role for paracrine factors in mixed populations of tumor cells of differing states of malignant progression.
A Chaotic Mixer for Magnetic Bead-Based Micro Cell Sorter (2004)

An efficient magnetic force driven mixer with simple configuration is designed, fabricated, and tested. It is designed to facilitate the mixing of magnetic beads and biomolecules in a microchannel, where mixing is unavoidably inefficient due to its low Reynolds number. With appropriate temporal variations of the force field, chaotic mixing is achieved, hence the mixing becomes effective. The mixing device consists of embedded microconductors as a magnetic field source and a microchannel that guides the streams of working fluid. It is demonstrated that a pair of integrated micro conductors provides a local magnetic field strong enough to attract nearby magnetic beads. Mixing of magnetic beads is accomplished by applying a time-dependent control signal to a row of conductors, at the Reynolds number of as low as 10/sup -2/. Two-dimensional numerical simulation has been performed to design the configuration of the channel and electrodes, which creates chaotic motion of beads. It is found that a simple two-dimensional serpentine channel geometry with the transverse electrodes is able to create the stretching and folding of material lines, which is a manifestation of chaos. The mixing pattern predicted by the simulation has been confirmed by both flow visualization and PTV (particle tracking velocimetry) in the chaotic mixer fabricated, which should greatly increase the attachment of beads onto the target biomolecules. The optimum frequency of applied control signal is searched by evaluating the Lyapunov exponent in both numerical and experimental particle tracking. It is found that the range of optimum Strouhal number is 5<St<12, which is defined by the channel width and the mean velocity.
Estimating the dominant person in multi-party conversations using speaker diarization strategies (2008)

In this paper, we apply speaker diarization strategies from a single source to the task of estimating the dominant person in a group meeting. Previous work has shown that speaking length is strongly correlated with perceived dominance. Here we investigate this in more depth by considering two dominance tasks where there is full and majority agreement amongst ground-truth annotators. In addition, we investigate how 24 different speed-up and algorithmic strategies, and source types lead to interesting outcomes when applied to dominance estimation. We obtained the best performance of 77% using our slowest scheme and a single distant microphone (SDM). Within the top 3 out of 24 performing experiments in both dominance tasks, we show that we can use the furthest SDM, with no prior knowledge of the number of speakers and the fastest diarization scheme, which performs 1.3 times faster than real-time.
Treadmill ECG test combined with myocardial perfusion imaging for evaluation of coronary artery disease: analysis of 340 cases. (2003)

Combined stress ECG and stress MPI perform "gate keeper" function for referral for angiography, as well as for risk stratification of those who already have coronary angiograms. Decisions for revascularization should be based on combined evaluation--a shift from stenosis- based to ischaemic--based evaluation. Success or failure of revascularization was also documented by this evaluation.
Preclinical evaluation of the antiproliferative potential of STI571 in Hodgkin's disease (2002)

STI571 is a selective tyrosine kinase inhibitor with proven therapeutic potential in malignancies expressing c-kit. A strong c-kit and stem cell factor expression was detected in the Hodgkin and Reed Sternberg cell line L1236, but not in 20 primary cases of classical Hodgkin's disease. Proliferation of L1236 cells was neither affected by addition of stem cell factor nor by neutralising anti-stem cell factor antibodies or STI571. Results suggest that patients with Hodgkin's disease may not benefit from therapy with STI571.
Temporal composability [real-time embedded systems] (2002)

The constructive design of dependable distributed real-time systems out of pre-validated components requires precise interface specifications of the components in the temporal domain and in the value domain. This paper focuses on the temporal specification of interfaces in composable distributed real-time systems and establishes four principles of composability (independent development of nodes, stability of prior services, performability of the communication system, and replica determinism). It presents the temporal firewall interface that forms a fully specified operational interface of a component. The paper explains how the temporal firewall interface supports the four principles of composability. It then classifies interfaces from the point of view of composability and shows how these interfaces correspond to the time-triggered and event-triggered communication paradigms.
Primary gastroduodenal prophylaxis with omeprazole for non‐steroidal anti‐inflammatory drug users (1998)

Omeprazole is an effective agent for gastroduodenal prophylaxis in patients taking NSAIDs. Its main effect is to reduce the rate of development of gastric and duodenal ulcers.
Age-Related Genetic Abnormalities: The Achilles‘ Heel for Customizing Therapy in Elderly Lung Cancer Patients (2007)

Aging and cancer are closely related, and DNA repair systems, mainly involving the nucleotide excision repair pathway, have an important caretaker function in both processes. More than half of non-small cell lung cancer patients are elderly, and the expression of some genes involved in the mitotic checkpoint, such as BubR1, declines with aging. Cisplatin-based chemotherapy is the standard treatment for advanced non-small cell lung cancer; if performance status is good, both elderly and younger patients can tolerate this treatment equally well. Customized cisplatin treatment, based on reduction of the nucleotide excision repair pathway function, could be an attractive approach, and the assessment of mitotic checkpoint genes can be used for selecting docetaxel treatment. Epidermal growth factor receptor (EGFR) mutations are particularly frequent in elderly lung cancer patients who are never-smokers and constitute an attractive target for treatment with EGFR tyrosine kinase inhibitors.
Combination use of statins and omega-3 fatty acids: an emerging therapy for combined hyperlipidemia (2006)

AbstractMany patients with coronary heart disease remain at risk for cardiovascular events despite treatment with statins, especially those with combined hyperlipidemia. Since the observation in the 1970s that Greenland Eskimos have reduced cardiovascular mortality despite a high-fat diet, the role of omega-3 fatty acids in human health has generated intense interest. Omega-3 fatty acids have been shown to provide cardiovascular benefits in secondary prevention and have a hypotriglyceridemic effect, as well as other beneficial biological properties. Combination therapy using statins and omega-3 fatty acids has been proposed as a method to improve both low-density lipoprotein cholesterol and triglycerides in patients with combined hyperlipidemia. In addition, combination therapy with omega-3 fatty acids may represent a way to reduce the residual cardiovascular risk seen after statin monotherapy. This review highlights the properties of omega-3 fatty acids and reviews the current evidence for their use alone and in combination with statins in reducing cardiovascular mortality.Keywords: cholesterolcombination therapycombined hyperlipidemiacoronary heart diseaseeicosapentanoic acidfish oilhypertriglyceridemialipidsomega-3 fatty acids
Repaglinide and Related Hypoglycemic Benzoic Acid Derivatives (1998)

The structure-activity relationships in two series of hypoglycemic benzoic acid derivatives (5, 6) were investigated. Series 5 resulted from meglitinide (3) when the 2-methoxy was replaced by an alkyleneimino residue. Maximum activity was observed with the cis-3, 5-dimethyl-piperidino (5h) and the octamethyleneimino (5l) residues. Series 6 resulted from the meglitinide analogon 4 bearing an inversed amido function when the 2-methoxy, the 5-fluoro, and the alpha-methyl residue were replaced by a 2-piperidino, a 5-hydrogen, and a larger alpha-alkyl residue, respectively. An alkoxy residue ortho to the carboxy group further increased activity and duration of action in the rat. The most active racemic compound, 6al (R4 = isobutyl; R = ethoxy), turned out to be 12 times more active than the sulfonylurea (SU) glibenclamide (1). Activity was found to reside predominantly in the (S)-enantiomers. Compared with the SUs 1 and 2 (glimepiride), the most active enantiomer, (S)-6al (AG-EE 623 ZW; repaglinide; ED50 = 10 micro/kg po), is 25 and 18 times more active. Repaglinide turned out to be a useful therapeutic for type 2 diabetic patients; approval was granted recently by the FDA and the EMEA. From investigations on the pharmacophoric groups in compounds of type 5 and 6, it was concluded that in addition to the two already known-the acidic group (COOH; SO2NH) and the amidic spacer (CONH; NHCO)-the ortho residue R1 (alkyleneimino; alkoxy; oxo) must be regarded as a third one. A general pharmacophore model suitable for hypoglycemic benzoic acid derivatives, SUs, and sulfonamides is proposed (Figure 6). Furthermore, from superpositions of low-energy conformations (LECs) of 1, 2, and (S)-6al, it was concluded that a common binding conformation (LEC II; Figure 10B) may exist and that differences in binding to the SU receptor and in the mechanism of insulin release between repaglinide and the two SUs may be due to specific hydrophobic differences.
A Novel Stereodivergent Synthesis of Optically Purecis- andtrans-3-Substituted Proline Derivatives (1997)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTA Novel Stereodivergent Synthesis of Optically Pure cis- and trans-3-Substituted Proline DerivativesN. André Sasaki, Michael Dockner, Angèle Chiaroni, Claude Riche, and Pierre PotierView Author Information Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France Cite this: J. Org. Chem. 1997, 62, 3, 765–770Publication Date (Web):February 7, 1997Publication History Received18 September 1996Published online7 February 1997Published inissue 1 February 1997https://pubs.acs.org/doi/10.1021/jo961790rhttps://doi.org/10.1021/jo961790rbrief-reportACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views444Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Alcohols,Mixtures,Post-translational modification,Pyrrolidines,Reaction products Get e-Alerts
Nano-Intrinsic True Random Number Generation (2017)

Recent advances in predictive data analytics and ever growing digitalization and connectivity with explosive expansions in industrial and consumer Internet-of-Things (IoT) has raised significant concerns about security of people's identities and data. It has created close to ideal environment for adversaries in terms of the amount of data that could be used for modeling and also greater accessibility for side-channel analysis of security primitives and random number generators. Random number generators (RNGs) are at the core of most security applications. Therefore, a secure and trustworthy source of randomness is required to be found. Here, we present a differential circuit for harvesting one of the most stochastic phenomenon in solid-state physics, random telegraphic noise (RTN), that is designed to demonstrate significantly lower sensitivities to other sources of noises, radiation and temperature fluctuations. We use RTN in amorphous SrTiO3-based resistive memories to evaluate the proposed true random number generator (TRNG). Successful evaluation on conventional true randomness tests (NIST tests) has been shown. Robustness against using predictive machine learning and side-channel attacks have also been demonstrated in comparison with non-differential readouts methods.
New salicylates as maintenance treatment in ulcerative colitis. (1994)

New salicylates as maintenance treatment in ulcerative colitis For decades sulphasalazine has been the only valuable non-corticosteroid drug in the treatment of ulcerative colitis. Then Truelove et al showed that the pharmacologically active moiety in sulphasalazine for the treat- ment of this disease was 5-aminosalicylic acid (5-ASA).' This resulted in new 5-ASA formulations for topical and oral use. 5-ASA enemas and suppositories seem to have the same efficacy as topical corticosteroids in active distal ulcerative colitis.2 3 Sulphasalazine is not as effective as corticosteroids for the treatment of widespread active disease, and there is no evidence that any of the newer salicylates is better in this respect.4 Because extensive, active ulcerative colitis must be treated aggressively so that it does not become severe and require surgery, systemic corticosteroids are on July 24, 2023 by guest.
GaAs/AlGaAs and InGaAs/AlGaAs MODFET inverter simulations (1986)

Although MODFET's have exhibited the fastest switching speed for any digital circuit technology, there is as yet no clear consensus on optimal inverter design rules. We therefore have developed a comprehensive MODFET device model that accurately accounts for such high gate bias effects as transconductance degradation and increased gate capacitance. The device model, which agrees with experimental devices fabricated in this laboratory, is used in the simulation of direct-coupled FET logic (DCFL) inverters with saturated resistor loads. Based on simulation results, the importance of large driver threshold voltage not only for small propagation delay times but for wide logic swings and noise margins is demonstrated. Furthermore, minimum delay times are found to occur at small supply voltages as seen experimentally. Both of these results are attributed to the reduction of detrimental high gate bias effects. The major effect of reducing the gate length on delay time is to decrease the load capacitance of the gate. Using 0.25-µm gates, delay times of 5 and 3.6 ps at 300 and 77 K, respectively, are predicted. Finally, the recently introduced In-GaAs/AlGaAs MODFET's are shown to have switching speeds superior to those of conventional GaAs/AlGaAs MODFET's.
The Zucker fatty rat as a genetic model of obesity and hypertension. (1989)

The association of hypertension with obesity has long been recognized; however, because of the lack of suitable animal models of obesity and hypertension, the pathogenesis of the high blood pressure associated with obesity remains poorly understood. We hypothesized that the Zucker fatty rat, a widely studied model of obesity and insulin resistance, might also be characterized by hypertension. Mean arterial pressure directly measured in the unanesthetized, unrestrained obese (fatty) Zucker rat was significantly greater than in two strains of nonobese control rats, the lean Zucker rat and the Lewis rat. The greater blood pressure in the obese rats was not dependent on hyperphagia or increased body weight per se since moderate caloric restriction, achieved by pair-feeding with lean rats, decreased weight gain but did not attenuate hypertension. Pair-fed obese rats retained less sodium than lean control rats, suggesting that greater blood pressure in the obese rats is not a consequence of increased renal retention of sodium. A unique feature of the Zucker strain is that the increased blood pressure appears to be specifically associated with the obese genotype. The findings suggest that the obese Zucker rat might provide a useful experimental model of obesity and hypertension.
Hexose‐6‐phosphate dehydrogenase determines the reaction direction of 11β‐hydroxysteroid dehydrogenase type 1 as an oxoreductase (2004)

The impact of hexose-6-phosphate dehydrogenase (H6PDH) on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity was investigated upon coexpression in HEK-293 cells. Confocal microscopy analysis indicated colocalisation of both enzymes at the lumenal side of the endoplasmic reticulum (ER) membrane. Functional analysis in intact cells revealed fivefold stimulation of 11beta-HSD1 oxoreductase activity and sixfold decrease of dehydrogenase activity upon coexpression with H6PDH, without changing kinetic parameters in cell lysates. Thus, H6PDH directly determines the reaction direction of 11beta-HSD1 in intact cells as an oxoreductase without changing intrinsic catalytic properties of 11beta-HSD1 by regenerating NADPH in the ER-lumen.
In vivo delivery of heat shock protein 70 accelerates wound healing by up‐regulating macrophage‐mediated phagocytosis (2006)

Injury causes tissue breakdown, which releases large quantities of intracellular contents into the extracellular space. Some of these materials are well-established activators of the immune system and include heat shock proteins (HSPs), uric acid, nucleotides, High Mobility Group Box-1 protein (HMGB-1), and DNA. Here, we show that in vivo delivery of HSPs into BALB/cJ mice with full-thickness wounds accelerates the rate of wound closure by 60% as compared with control-treated mice. The onset is rapid and the effect is sustained, dose dependent, and protein specific. Adoptive transfer of RAW264 macrophages pretreated with HSP70 into naïve recipients with a wound transfers the HSP-mediated effect on the rate of wound closure. Further, we demonstrate that part of the mechanism by which HSP70 accelerates wound closure is through the stimulation of macrophage-mediated phagocytosis of wound debris. Disabling the HSP70-mediated enhancement of phagocytosis abrogates the HSP-mediated acceleration of the healing process. These findings create two opportunities: one, therapeutic, wherein HSP70 could be used in the clinical management of wounds; and two, pathophysiologic, to decode signals by which the host defenses recognize and respond to injury.
The Spider Toxin ω-Aga IIIA Defines a High Affinity Site on Neuronal High Voltage-activated Calcium Channels (2000)

The spider toxin omega-agatoxin IIIA (omega-Aga-IIIA) is a potent inhibitor of high voltage-activated calcium currents in the mammalian brain. To establish the biochemical parameters governing its action, we radiolabeled the toxin and examined its binding to native and recombinant calcium channels. In experiments with purified rat synaptosomal membranes, both kinetic and equilibrium data demonstrate one-to-one binding of omega-Aga-IIIA to a single population of high affinity sites, with K(d) = approximately 9 pm and B(max) = approximately 1.4 pmol/mg protein. Partial inhibition of omega-Aga-IIIA binding by omega-conotoxins GVIA, MVIIA, and MVIIC identifies N and P/Q channels as components of this population. omega-Aga-IIIA binds to recombinant alpha(1B) and alpha(1E) calcium channels with a similar high affinity (K(d) = approximately 5-9 pm) in apparent one-to-one fashion. Results from recombinant alpha(1B) binding experiments demonstrate virtually identical B(max) values for omega-Aga-IIIA and omega-conotoxin MVIIA, providing further evidence for a one-to-one stoichiometry of agatoxin binding to calcium channels. The combined evidence suggests that omega-Aga-IIIA defines a unique, high affinity binding site on N-, P/Q-, and R-type calcium channels.