gebhart/patrepeval huggingface dataset:
Equine Atopic Dermatitis: Pathogenesis (2013)

Horses develop a number of skin and respiratory disorders that have been attributed to allergy. These include pruritic skin diseases, recurrent urticaria, and allergic rhinoconjunctivitis, and reactive airway disease. There is a complex interplay between the immune system and the nervous system, which promotes the sensation of itch. Th2 cytokines, particularly IL-31, directly stimulate itch by binding to their receptors on nerve fibres. Other pruritogenic mediators, including histamine, proteases, substance P, opioids, neurotrophins, and other neuroactive peptides, enhance the itch. The disease most like atopic dermatitis is the pruritus and dermatitis associated with Culicoides hypersensitivity and, in some horses, associated with intradermal or serum test reactions to environmental allergens. Barrier dysfunction is considered an integral part of the pathogenesis of atopic dermatitis. In fact, the skin barrier and the immune response are believed to be tightly linked.
The Stabilization Effect of Mesenchymal Stem Cells on the Formation of Microvascular Networks in a Microfluidic Device (2013)

There is a demand for three-dimensional (3D) angiogenesis model including endothelial cells (ECs) and mesenchymal stem cells (MSCs), which are known to differentiate into pericytes, to construct stabilized and matured microvascular networks in vitro. However, it remains to be elucidated how MSCs affected on ECs in the process of 3D angiogenesis. In this study, we utilized a microfluidic device to develop a 3D coculture system including human umbilical vein ECs and human MSCs, which allowed us to investigate the effects of MSCs on ECs in the context of 3D angiogenesis. A series of EC:MSC ratio was tested in the EC-MSC coculture. First, we confirmed that MSCs differentiated into pericytes by direct EC-MSC contacts. Next, we found that MSCs attenuated vascular sprout formation of ECs regardless of EC:MSC ratio in the early stage of 3D angiogenesis as well as extension of microvascular networks in the later stage. ECs and MSCs were also cultured under interstitial flow to enhance angiogenesis. However, the stabilization effects of MSCs on the extension of capillary structures were dominant over the promotion effects of the interstitial flow. These results indicate the stabilization effect of MSCs on the formation of microvascular networks in vitro. Although some HMSCs differentiated into pericytes and located around microvascular networks, vascular structures became thick over time in coculture. The 3D EC-MSC coculture model described in this study is useful to further investigate culture microenvironments for constructing stabilized and matured microvascular networks with aligning pericytes.
Recursive ensemble mutagenesis (1993)

We have developed a generally applicable experimental procedure to find functional proteins that are many mutational steps from wild type. Optimization algorithms, which are typically used to search for solutions to certain combinatorial problems, have been adapted to the problem of searching the 'sequence space' of proteins. Many of the steps normally performed by a digital computer are embodied in this new molecular genetics technique, termed recursive ensemble mutagenesis (REM). REM uses information gained from previous iterations of combinatorial cassette mutagenesis (CCM) to search sequence space more efficiently. We have used REM to simultaneously mutate six amino acid residues in a model protein. As compared to conventional CCM, one iteration of REM yielded a 30-fold increase in the frequency of 'positive' mutants. Since a multiplicative factor of similar magnitude is expected for the mutagenesis of additional sets of six residues, performing REM on 18 sites is expected to yield an exponential (30,000-fold) increase in the throughput of positive mutants as compared to random [NN(G,C)]18 mutagenesis.
The neurologic aspects of transcobalamin II deficiency (1992)

Thirty-four symptomatic cases of inherited transcobalamin II (TCII) deficiency were analysed in order to determine the frequency and nature of neurologic manifestations. In no instance was there definite evidence of a neurologic disorder at the time of presentation as a young infant. One child of 2 1/2 years transiently lost deep tendon reflexes at a time of suboptimal treatment. A syndrome of mental retardation and other neurologic manifestations was observed in three cases, all with the following in common: (1) an extended duration of illness of 2-17 years; (2) inadequate or not treatment with Cbl; (3) treatment with folic of folinic acid. TCII deficiency rarely if ever presents with neurologic manifestations. However, neurologic disorders can be produced subsequently by improper treatment.
Lipoprotein lipase enhances binding of lipoproteins to heparan sulfate on cell surfaces and extracellular matrix. (1992)

Lipoprotein lipase enhances binding at 4 degrees C of human plasma lipoproteins (chylomicrons, VLDL, intermediate density lipoprotein, LDL, and HDL3) to cultured fibroblasts and hepG-2 cells and to extracellular matrix. Heparinase treatment of cells and matrix reduces the lipoprotein lipase enhanced binding by 90-95%. Lipoprotein lipase causes only a minimal effect on the binding of lipoproteins to heparan sulfate deficient mutant Chinese hamster ovary cells while it promotes binding to wild type cells that is abolished after heparinase treatment. With 125I-LDL, lipoprotein lipase also enhances uptake and proteolytic degradation at 37 degrees C by normal human skin fibroblasts but has no effect in heparinase-treated normal cells or in LDL receptor-negative fibroblasts. These observations prove that lipoprotein lipase causes, predominantly, binding of lipoproteins to heparan sulfate at cell surfaces and in extracellular matrix rather than to receptors. This interaction brings the lipoproteins into close proximity with cell surfaces and may promote metabolic events that occur at the cell surface, including facilitated transfer to cellular receptors.
Pharmacokinetics and biodistribution of engineered single-chain antibody constructs of MAb CC49 in colon carcinoma xenografts. (1999)

The rapid clearance from the blood, higher tumor uptake and longer retention of the stable dimer of CC49 scFv make it an important agent for potential imaging and therapeutic applications.
Asymmetric Synthesis of an Axially Chiral Antimitotic Biaryl via an Atropo-Enantioselective Suzuki Cross-Coupling (2003)

A catalytic asymmetric synthesis of the axially chiral bridged biaryl (-)-2, a structural analogue of natural (-)-rhazinilam possessing original antimitotic properties, is described. The key step is an intermolecular asymmetric Suzuki coupling, furnishing the nonbridged biaryl (-)-6, precursor of (-)-2, with up to 40% ee using binaphthyl ligand 7a. Various known or new binaphthyl and ferrocenyl phosphines as well as phosphetanes were screened as ligands in this reaction, the conditions of which were optimized. The comparison with another Suzuki coupling system showed that 7a is the most versatile ligand described to date for this type of transformation. This work gives the first application of the asymmetric Suzuki coupling to a biologically relevant target.
Effect of Salinity Change on Biomass and Biochemical Composition of Nannochloropsis oculata (2012)

Salinity fluctuation is an important factor affecting outdoor microalgae culture. This investigation examined the effect of salinity change (Tr‐1:35–15 g/L, Tr‐2:35–25 g/L, Tr‐3:35–35 g/L, Tr‐4:35–45 g/L, and Tr‐5:35–55 g/L) on growth and the biochemical composition of Nannochloropsis oculata , a candidate for biodiesel production in indoor photo‐bioreactors. Results showed that the algae increased in absorbency and dry biomass as salinity decreased. When the salinity increased, the specific growth rate (SGR) of the algae decreased significantly. The salinity stress also affected the pigments of the algae, the chlorophyll‐ a , and carotenoid contents of the algae which decreased with the increase of salinity from 45 to 55 g/L. The fatty acid methyl esters (FAME) content (% of dry biomass) increased with the increase of salinity (e.g., Tr‐4 and Tr‐5). The algae was rich in C16:0 (palmitic acid), C16:1n‐7 (palmitoleic acid), and C20:5n‐3 (eicosapentaenoic acid), and C16:0 content increased with decreasing salinity from 35 to 15 g/L, but C16:1n‐7 content was high in all the treatments ranging from 25.25 ± 1.42% in Tr‐1 to 27.05 ± 1.13% in Tr‐5.
Whispering-gallery-mode electro-optic modulator and photonic microwave receiver (2003)

We report on the experimental observation of efficient all-resonant three-wave mixing using high-Q whispering-gallery modes. The modes were excited in a millimeter size toroidal cavity fabricated from LiNbO3. We implemented a low-noise resonant electro-optic modulator based on this wave mixing process. We observe an efficient modulation of light with coherent microwave pumping at 9 GHz with applied power of approximately 10 mW. Used as a receiver, the modulator allows us to detect nanowatt microwave radiation. Preliminary results with a 33-GHz modulator prototype are also reported. We present a theoretical interpretation of the experimental results and discuss possible applications of the device.
Synthesis of phosphoenolypyruvate and its use in ATP cofactor regeneration (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of phosphoenolypyruvate and its use in ATP cofactor regenerationBernard L. Hirschbein, Francois P. Mazenod, and George M. WhitesidesCite this: J. Org. Chem. 1982, 47, 19, 3765–3766Publication Date (Print):September 1, 1982Publication History Published online1 May 2002Published inissue 1 September 1982https://pubs.acs.org/doi/10.1021/jo00140a036https://doi.org/10.1021/jo00140a036research-articleACS PublicationsRequest reuse permissionsArticle Views1317Altmetric-Citations122LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Differences Between the Sexes in the Anatomy of the Anterior Condyle of the Knee (2009)

Background: Claims that there are dramatic differences in anterior condylar anatomy between the sexes have led to the design of total knee implants with thinner anterior condyles specifically for use in women. We had observed, in our patients, differences in anterior condylar anatomy that appeared to be highly variable and dependent on the size, height, and ethnicity of the patient as well as his or her sex. Because of this observed variability, we sought to determine if differences in anterior condylar anatomy between the sexes actually exist. Methods: Two hundred and twelve randomly selected magnetic resonance images (112 of men and 100 of women) were evaluated. The anterior condyle was defined as the area of bone anterior to the anterior femoral cortex, 10 mm above the joint line. The medial and lateral heights of the anterior condyles were measured in millimeters directly from magnetic resonance imaging data obtained in two planes. The so-called aspect ratio was calculated to determine whether patient size had an effect on the size of the anterior condyles. Results: On the basis of the numbers available, there was no significant difference (p = 0.16) between the sexes with regard to lateral condylar height. The average difference was only 0.5 mm. There was a significant difference (p = 0.001) between men and women with regard to medial condylar height. However, the average difference was only 1.1 mm. While the difference between the sexes with regard to anterior condylar height was nominal, the measurements were highly variable regardless of sex. On the basis of the numbers available, there were no significant differences between men and women with regard to the condylar aspect ratios. Conclusions: The difference in anterior condylar anatomy is mentioned as one of three reasons for the need for a so-called gender-specific knee implant. The aspect ratio reported here, which is a surrogate for patient size, seems to negate any difference in anterior condylar anatomy based on sex. We have shown that anterior condylar anatomy is highly variable regardless of sex. We believe that implants as well as surgical techniques should be designed with the variability of anterior condylar anatomy taken into account and with an attempt to reproduce such anatomy regardless of sex.
PEO-Based Block Copolymers and Homopolymers as Reactive Surfactants for AGET ATRP of Butyl Acrylate in Miniemulsion (2008)

Amphiphilic block copolymers poly(ethylene oxide)-b-polystyrene (PEO-PS-Br) with various molecular weights and poly(ethylene oxide) homopolymer (PEO-Br) were synthesized and used as macroinitiators and stabilizers (reactive “surfactants”) for activator generated by electron transfer atom transfer radical polymerization (AGET ATRP) of n-butyl acrylate (BA), in miniemulsion either with or without ethyl 2-bromoisobutyrate (EBiB) as co-initiator. Under both conditions, the reactions were well controlled and stable latexes were formed. In the absence of EBiB, polymer particles with diameter around 120−230 nm were obtained, and the particles contained polymers with molecular weight Mn = 16 000−25 000 g/mol and relatively low polydispersity (Mw/Mn = 1.2−1.4). In the presence of a low molecular weight initiator, EBiB, the amount of surfactant used can be reduced in the reaction (1.7−4 wt % vs monomer). The percent of initiating sites from EBiB was changed from 30% to 90%, and the majority of the obtained polymers were initiated by EBiB. Nevertheless, because of the covalent linking of the surfactants to polymer chains, no free surfactant was left in the reaction system. The final diameter of latexes stabilized by reactive surfactants was around 200−390 nm, the number-average molecular weight Mn of the polymers obtained was 10 000−25 000 g/mol, and the polydispersity index was around 1.2.
A human‐human hybridoma system based on a fast‐growing mutant of the ARH‐77 plasma cell leukemia‐derived line (1982)

A rapidly-growing, HAT-sensitive cell line LICR-LON-HMy2 has been derived from the ARH-77 human plasma cell leukemia-derived line. It lacks the enzyme hypoxanthine phosphoribosyltransferase (EC 2.4.2.8). Hybrids have been reproducibly made for more than a year and in independent laboratories with lymphocytes from tonsils, lymph nodes and peripheral blood and tonsil lymphocytes cultured with antigens. The parent line and hybrids are very robust in culture and double in 20-30 h. Hybrids clone easily and have stable karyotypes, most with modal numbers in the sixties. Stable Ig secretion patterns have been observed over 20-30 passages, and after cloning. It was estimated that about half the hybrids produce new immunoglobulin chains in addition to the parent cell line's IgG1 (kappa light chain). High, but not limiting, density hybridoma cultures (approximately 5 x 10(5) cells/ml) typically produce 0.25-2 micrograms/ml immunoglobulin per day, but some hybrids produce more. A high-secreting variant of LICR-LON-HMy2 has been derived. The LICR-LON-HMy2 line is human and is distinct from the HAT-sensitive human B cell-lines SKO-007 and GM 1500-6TG Al 2. It is available for distribution.
Inhibition of melanoma growth and metastasis by combination with (−)‐epigallocatechin‐3‐gallate and dacarbazine in mice (2001)

(-)-Epigallocatechin-3-gallate (EGCG), a major polyphenol in green tea, was shown to have cancer chemopreventive activity. In this study, we examined the antimetastatic effects of EGCG or the combination of EGCG and dacarbazine on B16-F3m melanoma cells in vitro and in vivo. First, the antimetastatic potentials of five green tea catechins were examined by soft agar colony formation assay, and the results show that EGCG was more effective than the other catechins in inhibiting soft agar colony formation. Second, EGCG dose-dependently inhibited B16-F3m cell migration and invasion by in vitro Transwell assay. Third, EGCG significantly inhibited the spread of B16-F3m cells on fibronectin, laminin, collagen, and Matrigel in a dose-dependent manner. In addition, EGCG significantly inhibited the tyrosine phosphorylation of focal adhesion kinase (FAK) and the activity of matrix metalloproteinase-9 (MMP-9). In animal experiments, EGCG alone reduced lung metastases in mice bearing B16-F3m melanomas. However, a combination of EGCG and dacarbazine was more effective than EGCG alone in reducing the number of pulmonary metastases and primary tumor growths, and increased the survival rate of melanoma-bearing mice. These results demonstrate that combination treatment with EGCG and dacarbazine strongly inhibits melanoma growth and metastasis, and the action mechanisms of EGCG are associated with the inhibition of cell spreading, cell-extracellular matrix and cell-cell interactions, MMP-9 and FAK activities.
Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3) (1995)

Many gene products associated with the cdk cell cycle kinases are thought to regulate the active kinase complex and thus regulate the transition points of the cell cycle. Genes encoding these proteins may potentially function as oncogenes or tumor suppressor genes. We describe the chromosomal mapping by FISH of the genes for several cdk-associated proteins including human CCNC (cyclin C) to 6q21, CCNE (cyclin E) to 19q12-->q13, CDKN1 (p21) to 6p21.2 and CDKN3 (KAP to 14q22). Some of these sites are near chromosomal translocations or LOH sites common to a variety of human tumors. The potential role for each of these genes in neoplasia is discussed.
Directed Transformation of Molecules to Solids: Synthesis of a Microporous Sulfide from Molecular Germanium Sulfide Cages (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDirected Transformation of Molecules to Solids: Synthesis of a Microporous Sulfide from Molecular Germanium Sulfide CagesO. M. Yaghi, Z. Sun, D. A. Richardson, and T. L. GroyCite this: J. Am. Chem. Soc. 1994, 116, 2, 807–808Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://doi.org/10.1021/ja00081a067RIGHTS & PERMISSIONSArticle Views1404Altmetric-Citations244LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (297 KB) Get e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
DNA Priming Prior to Inactivated Influenza A(H5N1) Vaccination Expands the Antibody Epitope Repertoire and Increases Affinity Maturation in a Boost-Interval–Dependent Manner in Adults (2013)

DNA priming improves the response to inactivated influenza A(H5N1) vaccination. We compared the immunogenicity of an H5 DNA prime (using strain A/Indonesia/5/2005) followed by an H5N1 monovalent inactivated vaccine boost at 4, 8, 12, 16, or 24 weeks to that of 2 doses of H5N1 monovalent inactivated vaccine in adults. Antibody epitope repertoires were elucidated by genome-fragment phage-display library analysis, and antibody avidities for HA1 and HA2 domains were measured by surface plasmon resonance. H5 DNA priming expanded the H5-specific antibody epitope repertoire and enhanced antibody avidity to the HA1 (but not the HA2) domain in an interval-dependent manner. Enhanced HA1 binding and avidity after an interval of ≥12 weeks between prime and boost correlated with improved neutralization of homologous and heterologous H5N1 strains. Clinical trials registration NCT01086657.
Reflux-free cannula for convection-enhanced high-speed delivery of therapeutic agents (2005)

The authors developed a new step-design cannula for CED that effectively prevents reflux in vivo and maximizes the distribution of agents delivered in the brain. Data in the present study show reflux-free infusion with a constant volume of distribution in the rat brain over a broad range of flow rates. Reflux-free delivery of liposomes into nonhuman primate brain was also established using the cannula. This step-design cannula may allow reflux-free distribution and shorten the duration of infusion in future clinical applications of CED in humans.
An algebra for probabilistic databases (1994)

An algebra is presented for a simple probabilistic data model that may be regarded as an extension of the standard relational model. The probabilistic algebra is developed in such a way that (restricted to /spl alpha/-acyclic database schemes) the relational algebra is a homomorphic image of it. Strictly probabilistic results are emphasized. Variations on the basic probabilistic data model are discussed. The algebra is used to explicate a commonly used statistical smoothing procedure and is shown to be potentially very useful for decision support with uncertain information.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>