gebhart/patrepeval huggingface dataset:
Sites in Myelin Basic Protein that React with Monoclonal Antibodies (1985)

The epitopes (antigenic sites) for seven monoclonal antibodies (MAbs) evoked in rats or mice by guinea pig or monkey myelin basic protein (BP) have been located in four different sequences of the BPs extracted from various species. Six of the MAbs were evoked by guinea pig BP. (1) One epitope, possibly a pair, is included within residues 1-14 of all BPs tested and reacts with two rat IgG MAbs. (2) A definite pair of overlapping epitopes includes the central Phe91-Phe92 sequence. One epitope is contained entirely within sequence 90-99 and reacts with a rat IgG MAb. The substitution of Ser in chicken BP for Thr97 destroys this epitope. The other epitope appears to include residues on the amino side of Phe44 and even of His32 and suggests some tertiary structure in BP. This epitope reacts with a mouse IgM MAb that does not recognize the chicken substitution. (3) The third epitope lies within residues 114-121, specifically including Trp118, and reacts with a rat IgG MAb. A cross-reacting epitope probably includes residues 44-45 in certain species (guinea pig and bovine but not rabbit). (4) Another pair of epitopes is located within residues 131-140 but is severely species-restricted. This region in guinea pig BP evoked a species-specific mouse IgM MAb. The same region in monkey BP evoked the seventh MAb, a mouse IgG, which reacts with human, chimpanzee, monkey, bovine, and rat-18.5 kDa BPs and to a lesser extent rabbit BP but not with guinea pig, pig, or chicken BPs. Some tertiary structure in guinea pig BP is also suggested by the reactivities with the IgM MAb. All of the MAbs react with myelin in histologic preparations, but the optimum method of preparation of the tissue varies with each.
Intracoronary Bone Marrow Cell Transfer After Myocardial Infarction (2006)

In this study, a single dose of intracoronary BMCs did not provide long-term benefit on LV systolic function after AMI compared with a randomized control group; however, the study suggests an acceleration of LV ejection fraction recovery after AMI by BMC therapy.
Effect of p47phoxgene deletion on ROS production and oxygen sensing in mouse carotid body chemoreceptor cells (2005)

Membrane potential in oxygen-sensitive type I cells in carotid body is controlled by diverse sets of voltage-dependent and -independent K(+) channels. Coupling of Po(2) to the open-closed state of channels may involve production of reactive oxygen species (ROS) by NADPH oxidase. One hypothesis suggests that ROS are produced in proportion to the prevailing Po(2) and a subset of K(+) channels closes as ROS levels decrease. We evaluated ROS levels in normal and p47(phox) gene-deleted [NADPH oxidase knockout (KO)] type I cells using the ROS-sensitive dye dihydroethidium (DHE). In normal cells, hypoxia elicited an increase in ROS, which was blocked by the specific NADPH oxidase inhibitor 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF, 3 mM). KO type I cells did not respond to hypoxia, but the mitochondrial uncoupler azide (5 microM) elicited increased fluorescence in both normal and KO cells. Hypoxia had no effect on ROS production in sensory and sympathetic neurons. Methodological control experiments showed that stimulation of neutrophils with a cocktail containing the chemotactic peptide N-formyl-Met-Leu-Phe (1 microM), arachidonic acid (10 microM), and cytochalasin B (5 microg/ml) elicited a rapid increase in DHE fluorescence. This response was blocked by the NADPH oxidase inhibitor diphenyleneiodonium (10 microM). KO neutrophils did not respond; however, azide (5 microM) elicited a rapid increase in fluorescence. Physiological studies in type I cells demonstrated that hypoxia evoked an enhanced depression of K+ current and increased intracellular Ca2+ levels in KO vs. normal cells. Moreover, AEBSF potentiated hypoxia-induced increases in intracellular Ca2+ and enhanced the depression of K+ current in low O(2). Our findings suggest that local compartmental increases in oxidase activity and ROS production inhibit the activity of type I cells by facilitating K+ channel activity in hypoxia.
A library of organic landscapes on filamentous phage (1996)

A billion-clone library of filamentous phage with different surface structures ("landscapes') was generated by fusing random octapeptides to the N-terminus of all 4000 copies of the major coat protein. Such a "landscape library' might include clones exhibiting emergent properties that inhere in the entire surface architecture, not in the peptides by themselves. Because the diverse surface landscapes are displayed on viable phage, they can be surveyed for exceedingly rare functions using microbiological selection methods. Clones with several emergent properties of the sort envisioned were successfully selected, suggesting that landscape libraries have promise as a novel source of nanomaterials with exploitable surface properties.
Codon context effects on nonsense suppression in human cells (1993)

Conference Article| November 01 1993 Codon context effects on nonsense suppression in human cells Robin Martin; Robin Martin * †Krebs Institute for Biomolecular Research, The University of Sheffield, PO Box 594, Firth Court, Western Bank, Sheffield S10 2UH, U.K. *To whom correspondence should be addressed. Search for other works by this author on: This Site PubMed Google Scholar Mary K. Phillips-Jones; Mary K. Phillips-Jones †Krebs Institute for Biomolecular Research, The University of Sheffield, PO Box 594, Firth Court, Western Bank, Sheffield S10 2UH, U.K. Search for other works by this author on: This Site PubMed Google Scholar Fiona J. Watson; Fiona J. Watson ‡Trends in Biotechnology, Elsevier Trends Journals, 68 Hills Road, Cambridge CB2 1LA, U.K. Search for other works by this author on: This Site PubMed Google Scholar Lindsey S. J. Hill Lindsey S. J. Hill †Krebs Institute for Biomolecular Research, The University of Sheffield, PO Box 594, Firth Court, Western Bank, Sheffield S10 2UH, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1993) 21 (4): 846–851. https://doi.org/10.1042/bst0210846 Article history Received: July 30 1993 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation Robin Martin, Mary K. Phillips-Jones, Fiona J. Watson, Lindsey S. J. Hill; Codon context effects on nonsense suppression in human cells. Biochem Soc Trans 1 November 1993; 21 (4): 846–851. doi: https://doi.org/10.1042/bst0210846 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1993 Biochemical Society1993 Article PDF first page preview Close Modal You do not currently have access to this content.
Characterization of Cylinder-Forming Block Copolymers Directed to Assemble on Spotted Chemical Patterns (2008)

Films of cylinder-forming polystyrene-block-poly(methyl methacrylate) (bulk intercylinder spacing ao) were directed to assemble on surfaces chemically patterned with a hexagonal array of spots (interspot spacing as). The block copolymer domain assembly was investigated as a function of the film thickness and the commensurability between ao and as. The domains assembled vertically away from and matched the spacing and hexagonal order of the underlying lithographically patterned chemical substrate, provided as was equal to or up to 6% larger than ao and the film thickness was ≤54 nm. In contrast, comparably poor assembly was obtained when as was 8% less than ao. When as was commensurate with ao, the cylinder diameter and eccentricity had a lower standard deviation and was more circular, respectively, than that of self-assembled cylinders on a neutral substrate.
Improvement of blood glucose control in Type 1 diabetic patients treated with lispro and multiple NPH injections (1999)

An MDI regimen using lispro combined with multiple NPH compared to a standard MDI regimen using soluble insulin reduced day-to-day blood glucose fluctuations, was generally preferred by patients and was associated with a reduced incidence of severe hypoglycaemia with no loss of overall control.
Reactive Oxygen Species Precede the ε Isoform of Protein Kinase C in the Anesthetic Preconditioning Signaling Cascade (2003)

APC is mediated by PKC-epsilon but not by PKC-delta. Furthermore, PKC activation probably occurs downstream of ROS generation in the APC signaling cascade.
The role of ICAM expression in immunity and disease. (1995)

The ICAM family consists of three members. All are ligands for LFA1, and ICAM1 can also bind Mac1. However, the complete repertoire of other ligands has not yet been defined. The ICAMs are not functionally redundant, since they have quite distinct expression profiles and can bind LFA1 differentially. ICAM3 is constitutively and abundantly expressed by all leucocytes, whereas ICAM1 and ICAM2 are expressed at very low levels. ICAM1 is rapidly upregulated by inflammatory cytokines. A current working model for the interplay of the ICAMs is that ICAM3 is the major ligand for LFA1 in the initiation of immune responses and that ICAM1 takes over after these early stages. ICAM3 not only functions as an adhesion molecule, but also is clearly emerging as a potent signalling molecule.
Air-stable, high-conduction solid electrolytes of arsenic-substituted Li4SnS4 (2013)

HSAB theory predicts the design of air-stable, high-conduction sulfide based solid electrolytes.
Worldwide trends in mortality from biliary tract malignancies (2002)

There has been a marked global increase in mortality from intrahepatic, but not extra-hepatic, biliary tract malignancies.
Essential role for interferon-gamma and interleukin-6 in autoimmune insulin-dependent diabetes in NOD/Wehi mice. (1991)

Experimental studies in vitro suggest that cytokines are important mediators in the pathogenesis of autoimmune insulin-dependent diabetes mellitus (IDDM). However, there is little evidence for the role of cytokines in vivo, either in humans or in the spontaneous animal models of IDDM such as the NOD mouse or BB rat. To address this question, we used the model of cyclophosphamide (CYP)-induced autoimmune diabetes in the NOD/Wehi mouse to examine for (a) the production of IFN-gamma and IL-6 from isolated islets, and (b) the effect of anti IFN-gamma or anti IL-6 monoclonal antibodies on the development of diabetes. After cyclophosphamide, the majority of these mice develop of mononuclear cell infiltrate (insulitis) which by 10-14 d is associated with beta cell destruction. IFN-gamma activity at low levels (2.7 +/- 0.3 U/ml) could be detected only in culture supernatants from islets isolated at day 7 post-cyclophosphamide. In contrast, IL-6 activity progressively increased from 457 +/- 44 U/ml at day 0 to 6,020 +/- 777 U/ml at day 10. Culture of islets with anti-CD3 monoclonal antibody resulted in a significant increase in IFN-gamma activity from 41 +/- 7 U/ml at day 0 to 812 +/- 156 U/ml at day 10. Mice given either anti-IFN-gamma or anti-IL-6 antibody had a significantly reduced (P less than 0.001) incidence of diabetes and especially with IFN-gamma, decreased severity of insulitis. We conclude that IFN-gamma and IL-6 have essential roles in the pathogenesis of pancreatic islet beta cell destruction in this model.
Orthogonal frequency division multiple access peak-to-average power ratio reduction using optimized pilot symbols (2002)

In this paper, optimized pilot symbol (OPS) technique is proposed to reduce the peak-to-average power ratio (PAPR) of orthogonal frequency division multiple access (OFDMA) systems. The proposed PAPR reduction scheme is designed for OFDMA systems where the subcarriers assigned to each user are selected in clusters of consecutive subcarriers. Using the proposed scheme, the PAPR can be significantly reduced while maintaining low complexity and high flexibility on the channel assignment.
E2A proteins maintain the hematopoietic stem cell pool and promote the maturation of myelolymphoid and myeloerythroid progenitors (2009)

Hematopoiesis is a tightly controlled process maintained by a small pool of hematopoietic stem cells (HSCs). Here, we demonstrate that the LT-HSC, MPP, premegakaryocytic/erythroid, Pre CFU-E, Pre GM, MkP, and granulocyte-macrophage compartments were all significantly reduced in E2A-deficient bone marrow. Despite a severe depletion of erythroid progenitors, the erythrocyte and megakaryocyte compartments were equivalent in E2A-deficient bone marrow as compared with wild-type mice. E2A-deficient HSCs also failed to efficiently maintain the HSC pool on serial transplantation, and we demonstrate that the E2A proteins regulate cell cycle progression of HSCs by regulating the expression of p21(Cip1), p27(Kip1), and the thrombopoietin receptor, known regulators of HSC self-renewal activity. Based on these observations, we propose that the E2A proteins promote the developmental progression of the entire spectrum of early hematopoietic progenitors and to suppress an erythroid specific program of gene expression in alternative cell lineages. Last, the data mechanistically link E2A, cell cycle regulators, and the maintenance of the HSC pool in a common pathway.
Microbial production, structure elucidation and bioconversion of sophorose lipids (1988)

Abstract Cultivation of Torulopsis bombicola ATCC 22214 on a mixture of glucose and oleic acid (A) or oleic acid alone (B) produced large amounts of sophorose lipids. In the case of A, 38 g/1 of crude product were finally isolated; fermentation B led to 77 g/1. After separation by MPLC and TLC, six glycolipids were obtained and identified by NMR and fast atom bombardment‐mass spectrometry (FAB‐MS). In general, a 17‐hydroxyocta‐decanoic acid at the C‐1’ ‐position and acetate groups at the C‐6’ ‐and C‐6’ ‐positions of sophorose were found as substituents in the lactone and acidic forms of these lipids. The composition of product from A was as follows: 62% of sophorolipid 1’,4’ ‐lactone 6’ ,6’ ‐diacetate (SL‐1), 4% of sophorolipid 1’,4’‐lactone 6’‐monoacetate (SL‐2), 4% of sophorolipid 1’,4 ’‐lactone (SL‐3), 4% of sophorolipid 1’,6’‐and l’,6’‐lactones (SL‐4a,b), 4% of sophorolipid 6’‐monoacetate acid (SL‐5), 4% of sophorolipid acid (SL‐6) and finally 17% of other lipids. In B, the principal lactone (40%) had a double bond in the fatty acid moiety; the other components were identical with the above products. Yields of 13% SL‐2 and of 35% lipids containing no carbohydrate were significant. SL‐1 was deacetylated to SL‐3 (yield: 25‐307c) using acetyl‐esterase in a two‐phase system (cyclohexane/water).
Mass Spectrometry Imaging Enriches Biomarker Discovery Approaches with Candidate Mapping (2013)

Integral to the characterization of radiation-induced tissue damage is the identification of unique biomarkers. Biomarker discovery is a challenging and complex endeavor requiring both sophisticated experimental design and accessible technology. The resources within the National Institute of Allergy and Infectious Diseases (NIAID)-sponsored Consortium, Medical Countermeasures Against Radiological Threats (MCART), allow for leveraging robust animal models with novel molecular imaging techniques. One such imaging technique, MALDI (matrix-assisted laser desorption ionization) mass spectrometry imaging (MSI), allows for the direct spatial visualization of lipids, proteins, small molecules, and drugs/drug metabolites-or biomarkers-in an unbiased manner. MALDI-MSI acquires mass spectra directly from an intact tissue slice in discrete locations across an x, y grid that are then rendered into a spatial distribution map composed of ion mass and intensity. The unique mass signals can be plotted to generate a spatial map of biomarkers that reflects pathology and molecular events. The crucial unanswered questions that can be addressed with MALDI-MSI include identification of biomarkers for radiation damage that reflect the response to radiation dose over time and the efficacy of therapeutic interventions. Techniques in MALDI-MSI also enable integration of biomarker identification among diverse animal models. Analysis of early, sublethally irradiated tissue injury samples from diverse mouse tissues (lung and ileum) shows membrane phospholipid signatures correlated with histological features of these unique tissues. This paper will discuss the application of MALDI-MSI for use in a larger biomarker discovery pipeline.
결혼이주여성 가족 건강성에 영향을 미치는 경로 (2009)

본 연구는 결혼이주여성 가족의 건강성에 영향을 미치는 변인들의 영향경로를 살펴보고자 하였다. 전국적으로 143명의 결혼 이주여성을 대상으로 하였으며, 그들을 대상으로 한국어수준, 사회적 지지, 부부관계만족도, 우울과 적응유연성, 가족건강성을 측정하였다. 경로분석 결과는 다음과 같다. 첫째, 결혼 이주여성의 한국어 수준이 가족건강성에 직접 영향을 미치고, 적응유연성과 결혼만족도를 통해 가족건강성에 간접적으로 영향을 미쳤다. 둘째, 결혼이주여성의 한국어 수준은 사회적 지지, 결혼만족도를 통해 가족건강성에 간접적으로 영향을 미쳤다. 이러한 연구결과에 근거하여 볼 때 결혼 이주 여성의 한국어수준은 결혼이주여성 가족의 건강성에 영향을 미치는 주요 변인이었다.
Activation of EphA receptors on CD4+CD45RO+ memory cells stimulates migration (2010)

We have demonstrated previously that binding of ephrin-A1 to EphA receptors on human CD4(+) and CD8(+) T cells stimulates migration. Two EphA receptors have been reported in T cells: EphA1 at the protein level and EphA4 at the mRNA level. In this study, we wanted to investigate the expression profile of these receptors in T cell subpopulations and to test if expression differences would affect the potential of cells to migrate upon ephrin-A1 binding. We have generated an anti-EphA4 mAb for expression analysis. Our data show that functional EphA4 is expressed on the cell surface of CD4(+) and CD8(+) T cells. In addition, EphA4 receptor expression is induced after overnight incubation in serum-free medium, in particular, on CD4(+)CD45RO(+) T cells. Migration of CD4(+) T cells in response to ephrin-A1 is observed for memory cells (CD45RO(+)) and much weaker for naïve cells (CD45RA(+)). A signaling complex associated with the EphA4 receptor has also been isolated and includes EphA1, the Src family kinases Fyn and Lck, Slp76, and Vav1. To conclude, T cells express EphA1 and EphA4 receptors. Expression differences of EphA4 are observed in subpopulations of CD4(+) T cells. This is related to the cell migration potential after ephrin-A1 binding.
Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex. (1997)

The Bmi1 gene has been identified as a mouse Polycomb group (Pc-G) gene implicated in the regulation of Hox gene expression. Here we describe the characterization of a Bmi binding protein Mph1, which shares similarity to Drosophila polyhomeotic. Coimmunoprecipitation experiments indicate that Bmi1 and Mph1, as well as the Mel18 and M33 proteins described previously, are constituents of a multimeric protein complex in mouse embryos and human cells. A central domain of Bmi1 interacts with the carboxyl terminus of Mph1, whereas a conserved alpha-helical domain in the Mph1 protein is required for its homodimerization. Transgenic mice overexpressing various mutant Bmi1 proteins demonstrate that the central domain of Bmil is required for the induction of anterior transformations of the axial skeleton. Bmi1, M33, and Mph1 show an overlapping speckled distribution in interphase nuclei. These data provide molecular evidence for the existence of a mammalian Polycomb complex.
Engineered Fit-For-Purpose Cement System to Withstand Life-Of-The-Well Pressure and Temperature Cycling (2017)

Abstract Due to pressure and temperature changes during the life of the well (from drilling to production); globally many Casing-Casing Annular (CCA) leaks are observed in the annuli between surface and intermediate casings. A new approach to well cementing had to be developed, and advanced cement technologies considered, taking into account both short and long term design parameters. To assess the effect of pressure and temperature cycling on the long-term well integrity, finite element modeling of sections of interest was performed to analyze the stresses applied on the cement sheath throughout the life of the well. Such modeling requires detailed knowledge of the pressure and temperature cycles, as well as the mechanical properties of the formations, casings and cement. The output is a failure analysis of the cement sheath in terms of compression, tension and micro annulus development. Once the failure mechanisms are well understood, further sensitivity analyses permit to select the most adequate cement system for the application, based on target windows of compressive and tensile strengths, Young's modulus, Poisson's Ratio, and expansion capacity. The paper will discuss a case study where a fit-for-purpose durable cement system was designed to meet the required mechanical properties as suggested by the stress modeling performed on a candidate well. It was placed as a Tail slurry on the 9 5/8" Tieback casing to provide 1000 ft. of long-term hydraulic isolation in the cased annulus should the liner top packer fail to seal the high-pressure water formation below. A post-job analysis using job data and cement evaluation logs showed that placement was executed as per design and that the durable cement system provided excellent bonding and annular coverage. The well was later drilled to total depth, completed, fractured and delivered to production without CCA pressure at the wellhead. The new approach consists of performing finite elements stress modeling during the well planning phase to custom design cement systems that will withstand the anticipated loads.
Pharmacokinetics of Taxol and Doxorubicin Administered Alone and in Combination by Continuous 72-Hour Infusion (1994)

Journal Article Pharmacokinetics of Taxol and Doxorubicin Administered Alone and in Combination by Continuous 72-Hour Infusion Get access Stacey L. Berg, Stacey L. Berg Pediatric Branch, National Cancer InstituteBethesda, Md Correspondence to : Stacey L. Berg, M.D.., Pediatric Branch, Bldg. 10, Rm. 13N240, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD 20892. Search for other works by this author on: Oxford Academic PubMed Google Scholar Kenneth H. Cowan, Kenneth H. Cowan Medicine Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar Frank M. Balis, Frank M. Balis Pediatric Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar Jason S. Fisherman, Jason S. Fisherman Medicine Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar Andrea M. Denicoff, Andrea M. Denicoff Medicine Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar Marianne Hillig, Marianne Hillig Medicine Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar David G. Poplack, David G. Poplack Pediatric Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar Joyce A. O'Shaughnessy Joyce A. O'Shaughnessy Medicine Branch, National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 86, Issue 2, 19 January 1994, Pages 143–145, https://doi.org/10.1093/jnci/86.2.143 Published: 19 January 1994 Article history Received: 12 May 1993 Revision received: 23 September 1993 Accepted: 28 October 1993 Published: 19 January 1994