gebhart/patrepeval huggingface dataset:
Hydrogen rearrangement in molecular ions of alkyl benzenes: Mechanism and time dependence of hydrogen migrations in molecular ions of 1, 3‐diphenylpropane and deuterated analogues (1978)

Abstract Hydrogen migrations in the molecular ions of 1,3‐diphenylpropane, preceding the fragmentations to [C 7 H 7 ] + and [C 7 H 8 ] + ions, have been investigated by use of deuterated derivatives. By comparing the distribution of deuterium labels in the [C 7 (H, D) 8 ] + products from metastable molecular ions with the distribution patterns calculated for various exchange models, it is shown that the H migrations occur by two processes linked by a common intermediate: (i) exchange between hydrogen isotopes at the γ‐methylene group and at the ortho positions of the phenyl group: (ii) exchange between hydrogen isotopes at the ortho and orthó positions in the intermediate. In these mechanisms the eight hydrogen isotopes at both benzylic positions and both the ortho and orthó positions of 1,3‐diphenylpropane participate in a mutual exchange. A statistical equipartition of the hydrogen isotopes at these eight positions is not reached in metastable molecular ions, however. The distribution pattern of [C 7 (H, D) 8 ] + ions from the deuterium labelled compounds as a function of the mean number n of exchange cycles has been calculated according to this reaction model and compared with experimental results for unstable molecular ions, generated by 70 eV and 12 eV electrons, respectively, and metastable molecular ions. Good agreement is obtained for all compounds and n = 0.4–0.8 for unstable molecular ions and n = 5–8 for metastable ions. Therefore, the hydrogen exchange in the molecular ion of 1,3‐diphenylpropane is a rather slow process. These results firmly establish the isomerization reaction involving the conversion of the molecular ion of 1,3‐diphenylmethane to the intermediate and hence to the molecular ion of 7‐(2‐phenylethyl)‐5‐methylene cyclohexa‐1,3‐diene and preceding the fragmentations. The postulated intermediate is a true one which corresponds to a s̀‐complex type ion and which fragments to [C 7 H 8 ] + ions. Surprisingly, no isomerizations of the intermediate by hydrogen shifts within the protonated aromatic system (‘ring walks’) are observed.
Transcribing Meetings With the AMIDA Systems (2011)

In this paper, we give an overview of the AMIDA systems for transcription of conference and lecture room meetings. The systems were developed for participation in the Rich Transcription evaluations conducted by the National Institute for Standards and Technology in the years 2007 and 2009 and can process close talking and far field microphone recordings. The paper first discusses fundamental properties of meeting data with special focus on the AMI/AMIDA corpora. This is followed by a description and analysis of improved processing and modeling, with focus on techniques specifically addressing meeting transcription issues such as multi-room recordings or domain variability. In 2007 and 2009, two different strategies of systems building were followed. While in 2007 we used our traditional style system design based on cross adaptation, the 2009 systems were constructed semi-automatically, supported by improved decoders and a new method for system representation. Overall these changes gave a 6%-13% relative reduction in word error rate compared to our 2007 results while at the same time requiring less training material and reducing the real-time factor by five times. The meeting transcription systems are available at www.webasr.org.
The Caenorhabditis elegans gene lin-10 is broadly expressed while required specifically for the determination of vulval cell fates. (1990)

As a first step in a molecular dissection of the pathway controlling the determination of vulval cell fates in Caenorhabditis elegans, we have analyzed the gene lin-10. We show that loss-of-function mutations in this gene specifically prevent the induction of vulval cell lineages and result instead in the expression of hypodermal cell lineages. We isolated a transposon-insertion allele of lin-10 and used it to clone a genomic region that contains the lin-10 locus. The location of lin-10 within this region was determined by identifying a transcript affected by three independent lin-10 mutations and by delimiting the minimal segment of DNA sufficient to rescue the lin-10 mutant phenotype in germ line transformation experiments. The predicted lin-10 protein sequence is not similar to sequences in current data bases, suggesting that lin-10 defines a novel class of gene involved in the specification of cell fates. Although our genetic studies indicate that lin-10 is required specifically for the determination of vulval cell fates, lin-10 transcripts are present in cells other than vulval precursor cells. This result suggests that lin-10 may have a general but redundant role in development, functioning in diverse cell lineages to control cell fates. Alternatively, lin-10 may function specifically in vulval development, in which case lin-10 activity could be regulated at a post-transcriptional level or could have biological consequences only in conjunction with the products of other genes.
Maintenance of hepatic differentiation by hepatocyte attachment peptides derived from laminin chains (2011)

Hepatocytes rapidly lose hepatic functions upon isolation from liver, perhaps due to disrupted cell/matrix interactions. The matrix macromolecule laminin-111 consists of three chains, α1, β1, and γ1; it is a major component of Matrigel, which can maintain hepatic differentiation. We previously showed that the A13 peptide (RQVFQVAYIIIKA, α1 chain 121-133) derived from mouse laminin α1 exhibits hepatocyte attachment activity and maintains hepatic differentiation. Here, we sought to identify hepatocyte adhesive sequences from the mouse laminin β1 and γ1 chains using 22 synthetic peptides that show biological activity for fibrosarcoma cells. Nine peptides showed hepatocyte attachment activity. Of these, B160 (VILQQSAADIAR, β1 chain 1607-1618), and C16 (KAFDITYVRLKF γ1 chain 139-150) exhibited the most potent activity. Hepatocytes cultured on both peptides also maintained expression of albumin, tyrosine aminotransferase, tryptophan-2,3-dioxygenase, and cytochrome P450. The morphology of hepatocytes on both peptides was a rounded shape typical for hepatic differentiation. We also characterized the nature of adhesion to the peptides. Heparin and EDTA inhibited cell attachment to both peptides, suggesting that hepatocyte attachment to the peptides was mediated by multiple receptors. The identification of active sequences regulating hepatic functions may facilitate the design of hepatocyte culture substrata that can regulate specific cellular behaviors in the context of a bioartificial liver.
Structural Analogs of Human Insulin-like Growth Factor (IGF) I with Altered Affinity for Type 2 IGF Receptors (1989)

We have used site-directed mutagenesis of a synthetic gene for insulin-like growth factor (IGF) I to prepare three analogs in which specific residues in the A region are replaced with the corresponding residues in the A chain of insulin. The analogs are [Ile41, Glu45, Gln46, Thr49, Ser50, Ile51, Ser53, Tyr55, Gln56]IGF I (A chain mutant), in which residue 41 is changed from threonine to isoleucine and residues 42 to 56 of the A region are replaced, [Thr49, Ser50, Ile51]IGF I, and [Tyr55, Gln56]IGF I. These analogs are all equipotent to IGF I at the type 1 IGF receptor in human placental membranes, and in stimulating the incorporation of [3H]thymidine into DNA in the rat vascular smooth muscle cell line A10. However, the A chain mutant and [Thr49, Ser50, Ile51]IGF I have greater than 20-fold lower relative affinity for the type 2 IGF receptor of rat liver membranes, respectively. In contrast, [Tyr55, Gln56]IGF I has 7-fold higher affinity than IGF I for the type 2 IGF receptor. Residues 49, 50, and 51 in IGF I are Phe-Arg-Ser and are strictly conserved in IGF II. Residues 55 and 56 of IGF I and the corresponding residues in IGF II are Arg-Arg and Ala-Leu, respectively. Thus, the presence of the charged residues at these positions in IGF I appears to be responsible, in part, for the lower affinity of IGF I for the type 2 IGF receptor. In addition to the alterations in affinity for the type 2 IGF receptor, the A chain mutant has a 7-fold increase in affinity for insulin receptors, and [Thr49, Ser50, Ile51]IGF I has a 4-fold lower affinity for acid-stable human serum binding protein. These data strongly suggest that specific determinants in the A region of IGF I are important for maintaining binding to the type 2 IGF receptor, and that these determinants are different from those required for maintaining high affinity for the type 1 IGF receptor.
Advanced Materials for Banknote Applications (2001)

Australia has spearheaded the worldwide struggle against counterfeiting banknotes over the past 30 years. In the first stage polymer banknotes with transparent windows that contain an optically variable devices (OVDs) were issued. New developments comprise (a) the “self-veryfying banknote” where the interaction of features imprinted in the window e.g., an optical lens, and in another region of the note e.g., microtext only legible under this lens, prove the banknote's authenticity, (b) elaborate inks which for example reveal hidden images under special angles or light intensity, and diffractive OVD elements. Despite the great progress made, the quest for more forgery-proof money remains a continuing effort.
Comparative study of gated single crystal silicon and polysilicon field emitters (1997)

Both single crystal silicon and polysilicon are attractive emitter materials for displays using cold field emission. Although single crystal silicon has been under investigation for some time, by contrast, interest in polysilicon is more recent. Using state of the art fabrication techniques including high resolution electron beam lithography and plasma dry etch both single crystal silicon and polysilicon have been processed to fabricate sharp and uniform gated emitter arrays. We report in this article a comparative study of material aspects, processing, and emission properties of the two materials.
Prognostic significance of EpCAM expression in human esophageal cancer (2007)

EpCAM is a 40-kDa epithelial transmembrane glycoprotein that has a well-known role in Ca2+ -independent homophilic cell-cell adhesion. Recently, correlations between EpCAM expression and clinicopathological features have been investigated in various cancers. We examined EpCAM expression in surgical specimens from esophageal cancer patients (n = 138), using real-time RT-PCR, immunohistochemistry and ELISA. The mean expression level of EpCAM mRNA in tumor tissues was significantly higher than that in corresponding normal tissues (P < 0.0001). Immunohistochemically, positive staining for EpCAM was found in 135 (97.8%) of the 138 primary tumor specimens. EpCAM expression was correlated with tumor depth (P = 0.0005), stage (P = 0.0037), blood-vessel invasion (P = 0.0397) and infiltrative growth pattern (P = 0.0015). The survival rates of patients with tumors with high EpCAM expression was significantly higher than those for patients with tumors with low EpCAM expression (P = 0.0213). Furthermore, the serum EpCAM levels of patients with esophageal cancer were significantly higher than those of normal volunteers (P = 0.0221). The survival rates of patients with a high EpCAM level in the peripheral vein were also significantly higher than those for patients with a low serum EpCAM level (P = 0.0291). The serum EpCAM level in the peripheral vein was independently associated with prognosis (P = 0.0074; hazard ratio 7.40). Tumor-specific EpCAM expression and release into the circulation may serve as effective immunotherapy in esophageal cancer patients.
Characterization of chemisorbed hyaluronic acid directly immobilized on solid substrates (2004)

Hyaluronic acid (HA) has a number of potential biomedical applications in drug delivery and tissue engineering. For these applications, a prerequisite is to understand the characteristic of HA films directly immobilized to solid substrates. Here, we demonstrate that high molecular weight HA can be directly immobilized onto hydrophilic substrates without any chemical manipulation, allowing for the formation of an ultrathin chemisorbed layer. Hyaluronic acid is stabilized on these surfaces through hydrogen bonding between the hydrophilic moieties in HA [such as carboxylic acid (-COOH) or hydroxyl (-OH) groups] with silanol (-SiOH), carboxylic acid or hydroxyl groups on the hydrophilic substrates. Despite the water solubility, the chemisorbed HA layer remained stable on glass or silicon oxide substrates for at least 7 days in phosphate-buffered saline. Furthermore, HA immobilized on silicon and other dioxide surfaces in much higher quantities than other polysaccharides including dextran sulfate, heparin, heparin sulfate, chondroitin sulfate, dermatan sulfate, and alginic acid. This behavior is related to the molecular entanglement and intrinsic stiffness of HA as a result of strong internal and external hydrogen bonding as well as high molecular weight. These results demonstrate that HA can be used to coat surfaces through direct immobilization.
Three‐Dimensionally Ordered Macroporous Polymeric Materials by Colloidal Crystal Templating for Reversible CO2 Capture (2013)

Abstract The design and preparation of porous materials with controlled structures and functionalities is crucial to a variety of absorption‐ or separation‐relevant applications, including CO 2 capture. Here, novel functional polymeric materials with three‐dimensionally ordered macroporous (3DOM) structures are prepared by using colloidal crystals as templates using relatively simple, rapid, and inexpensive approaches. These ordered structures are used for the reversible CO 2 capture from ambient air by humidity swing. Typically, the colloidal crystal template is synthesized from polymer latex particles of poly(methyl methacrylate) (PMMA) or polystyrene (PS). To maintain the functionality of the material, it is important to prevent the porous structure collapsing, which can occur by the hydrolysis of the ester bonds in conventional crosslinkers under basic conditions. This hydrolysis can be prevented by using a water‐soluble crosslinker containing two quaternary ammonium moieties, which can be used to prepare stable porous crosslinked polymers with the monomer (vinylbenzyl)trimethylammonium chloride (VBTMACl) and using a PMMA‐based colloidal crystal template. The hydroxide‐containing monomer and dicationic crosslinker are synthesized from their chloride precursors, avoiding the ion‐exchange step which causes shrinkage of the pores. An analysis of different methods for infiltrating the monomer solution into the colloidal crystal template shows that infiltration using capillary forces leads to fewer defects than infiltration under a partial vacuum. In addition, functional macroporous films with micrometer thickness are prepared from a template of PS‐based colloidal crystals in a thin film. In general, the colloidal crystal templated materials showed improved CO 2 absorption/desorption rates and swing sizes compared to a commercially available material with similar functional groups. This work could easily be extended to create a new generation of ordered macroporous polymeric materials with tunable functionalities for other applications.
Branching ratio measurement of aΛsystem inTm3+:YAGunder a magnetic field (2007)

A three-level $\ensuremath{\Lambda}$ system in ${\mathrm{Tm}}^{3+}$ doped YAG crystal is experimentally investigated in the prospect of quantum information processing. Zeeman effect is used to lift the nuclear spin degeneracy of this ion. In a previous paper [de Seze et al., Phys. Rev. B 73, 085112 (2006)] we measured the gyromagnetic tensor components and concluded that adequate magnetic field orientation could optimize the optical connection of both ground state sublevels to each one of the excited state sublevels, thus generating $\ensuremath{\Lambda}$ systems. Here we report on the direct measurement of the transition probability ratio along the two legs of the $\ensuremath{\Lambda}$. Measurement techniques combine frequency selective optical pumping with optical nutation or photon echo processes.
The RNA Component of Human Telomerase (1995)

Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5'-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG)n. Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.
Rigid Body Registration (2003)

This chapter opens with a discussion of how images are transformed via the process of resampling. This chapter is about rigid registration of images and therefore it describes the parameterization of rigid body transformations as a subset of the more general affine transformations. Image registration is important in many aspects of functional image analysis. In imaging neuroscience, particularly for fMRI, the signal changes due to any hæmodynamic response can be small compared to apparent signal differences that can result from subject movement. Subject head movement in the scanner cannot be completely eliminated, so retrospective motion correction is performed as a preprocessing step. This is especially important for experiments where subjects may move in the scanner in a way that is correlated with the different conditions. It also focuses on the methods of rigid body registration, in both intra and intermodality contexts. Intramodality registration implies registration of images acquired using the same modality and scanning sequence or contrast agent, whereas intermodality registration allows the registration of different modalities. © 2004 Elsevier Inc. All rights reserved.
Contemporary issues in rate‐adaptive pacing (1997)

This review addresses a number of questions pertaining to contemporary rate-adaptive pacemakers that include the role of dual sensor systems, sensor blending and cross-checking, automaticity, nonrate-related sensor functions, prevention of arrhythmias, and several other areas of clinical importance.
Critical Role for Microglial NADPH Oxidase in Rotenone-Induced Degeneration of Dopaminergic Neurons (2003)

Increasing evidence has suggested an important role for environmental toxins such as pesticides in the pathogenesis of Parkinson's disease (PD). Chronic exposure to rotenone, a common herbicide, reproduces features of Parkinsonism in rats. Mechanistically, rotenone-induced dopaminergic neurodegeneration has been associated with both its inhibition of neuronal mitochondrial complex I and the enhancement of activated microglia. Our previous studies with NADPH oxidase inhibitors, diphenylene iodonium and apocynin, suggested that NADPH oxidase-derived superoxide might be a major factor in mediating the microglia-enhanced rotenone neurotoxicity. However, because of the relatively low specificity of these inhibitors, the exact source of superoxide induced by rotenone remains to be further determined. In this study, using primary mesencephalic cultures from NADPH oxidase--null (gp91phox-/-) or wild-type (gp91phox+/+) mice, we demonstrated a critical role for microglial NADPH oxidase in mediating microglia-enhanced rotenone neurotoxicity. In neuron--glia cultures, dopaminergic neurons from gp91phox-/- mice were more resistant to rotenone neurotoxicity than those from gp91phox+/+ mice. However, in neuron-enriched cultures, the neurotoxicity of rotenone was not different between the two types of mice. More importantly, the addition of microglia prepared from gp91phox+/+ mice but not from gp91phox-/- mice to neuron-enriched cultures markedly increased rotenone-induced degeneration of dopaminergic neurons. Furthermore, apocynin attenuated rotenone neurotoxicity only in the presence of microglia from gp91phox+/+ mice. These results indicated that the greatly enhanced neurotoxicity of rotenone was attributed to the release of NADPH oxidase-derived superoxide from activated microglia. This study also suggested that microglial NADPH oxidase may be a promising target for PD treatment.
Treatment of hyperkinetic movement disorders with tetrabenazine: A double‐blind crossover study (1982)

Tetrabenazine, a presynaptic monoamine depleting agent, has been reported to have an ameliorating effect in a variety of hyperkinetic movement disorders. In a double-blind crossover trial of tetrabenazine versus placebo, 19 patients with a variety of hyperkinetic movement disorders were evaluated. During the evaluation period, all but 4 patients were treated for three or more weeks at a maximum dosage of 200 mg per day. The patients were examined and rated using clinical assessment of hyperkinesia, and movies of their activities were randomized and rated by an independent group of neurologists. A good correlation was found between the clinical examination scale and the film analysis score. Improvement was seen in all 4 patients with tardive dyskinesia, 4 of 6 patients with Meige disease, and 5 of 6 patients with other dystonias. One patient with Huntington disease showed marked improvement and 2 patients with congenital choreoathetosis showed only mild improvement. The most frequent side effects included daytime drowsiness, drooling or sialorrhea, insomnia, restlessness and anxiety, parkinsonian features, and mild postural hypotension. The adverse effects resolved with continued administration or with reduction in dosage. Tetrabenazine is a useful and safe therapeutic agent in some patients with hyperkinetic movement disorders.