Gene Silencing by RNA Interference : Technology and Application (2004)

Gene silencing by RNA interference and the role of small interfering RNAs N.J. Caplen, National Institutes of Health, Maryland, USA Basics of siRNA design and chemical synthesis Q. Boese, A. Reynolds, and W.S. Marshall, Dharmacon, Inc. Colorado, USA Oligonucleotide scanning arrays in the design of small interfering RNAs V.M. Macaulay and M. Sohail, Weatherall Institute of Molecular Medicine and University of Oxford, England, UK siRNA production by in vitro transcription O. Donze, P. Dudek, and D. Picard, Universite de Geneve and Apotech Corporation, Switzerland Production of siRNAs with the application of deoxyribozymes G. Doran and M. Sohail, University of Oxford, England, UK Production of siRNA in vitro by enzymatic digestion of double-stranded RNA F. Buchholz, D. Drechsel, M. Ruer, and R. Kittler, Max Planck Institute, Dresden, Germany Plasmid-mediated intracellular expression of siRNAs R. Wadhwa, M. Sano, M. Miyagishi, and K. Taira, National Institute of Advanced Industrial Science and Technology, Tsukuba, and University of Tokyo, Japan Lentiviral vector-mediated delivery of si/shRNA, D.S. An, X-F. Qin, Vincent Auyeung, D. Baltimore, and I.S.Y. Chen, University of California-Los Angeles, UT MD Anderson Cancer Center in Texas, and California Institute of Technology, Pasadena, USA Exogenous siRNA delivery: protocols for optimizing delivery to cells A.J. Hollins, S.P. Fox, and S. Akhtar, Cardiff University, Wales, UK RNAi in Drosophila cell cultures, M. Bettencourt-Dias, R. Sinka, L. Frenz, and D. Glover, University of Cambridge. and Cyclacel, Ltd., England, UK RNAi in Caenorhabditis elegans F. Simmer and R.H.A. Plasterk, Center for Biomedical Genetics, Utrecht, The Netherlands Delivery of RNAi reagents in C. elegans by microinjection P. Appleford and A. Woollard, University of Oxford, England, UK RNAi in Drosophila embryos R.W. Williams, University of California, Berkeley, USA RNAi in Xenopus laevis oocytes E. Gordon and G.W. Abbott, Cornell University, New York, USA RNAi in Xenopus embryos K.H. Kok and D-Y. Jin, University of Hong Kong, People's Republic of China An RNAi-based genomic library for forward genetics in the African Trypanosome J.C. Morris, Z. Wang, S.A. Motyka, M.E. Drew, and P.T. Englund, Clemson University, South Carolina, Massachusetts Institute of Technology, Massachusetts, Johns Hopkins University, Maryland, and Washington University, Missouri, USA RNAi in the malaria parasite Plasmodium A. Bruning-Richardson and G.A. McConkey, University of Leeds, England, UK Silencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches P.V.N. Dasaradhi, A. Mohmmed, M.J. Hossain, V.S. Chauhan, and P. Malhotra, International Center for Genetic Engineering and Biotechnology, New Delhi, India RNA in transgenic animal models H. Hasuwa & M. Okabe, Osaka University, Japan RNA interference in mouse model, E. Song, P. Stern, D. Palliser, Luk Van Parijs and J. Lieberman, Harvard University, Massachusetts, USA Virus-induced RNA silencing in plants M. Figlerowicz, M. Barciszewska-Pacak, B. Grabowska, J. Wojciechowicz, and A. Pacak, Adam Mickiewicz University, Poznan, University of Warmia and Mazury, Olsztyn, and Polish Academy of Sciences, Poznan, Poland RNA silencing in plants - biolistic delivery of RNAi reagents U. Klahre and F. Meins, Novartis Research Foundation, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland Agro-infiltration: a versatile tool for RNAi studies in plants D. Silhavy, Agricultural Biotechnology Center, Godollo, Hungary