gebhart/patrepeval huggingface dataset:
Four-Dimensional Heteronuclear Triple-Resonance NMR Spectroscopy of Interleukin-1β in Solution (1990)

A method is presented that dramatically improves the resolution of protein nuclear magnetic resonance (NMR) spectra by increasing their dimensionality to four. The power of this technique is demonstrated by the application of four-dimensional carbon-13--nitrogen-15 (13C-15N)--edited nuclear Overhauser effect (NOE) spectroscopy to interleukin-1 beta, a protein of 153 residues. The NOEs between NH and aliphatic protons are first spread out into a third dimension by the 15N chemical shift of the amide 15N atom and subsequently into a fourth dimension by the 13C chemical shift of the directly bonded 13C atoms. By this means ambiguities in the assignment of NOEs between NH and aliphatic protons that are still present in the three-dimensional 15N-edited NOE spectrum due to extensive chemical shift overlap and degeneracy of aliphatic resonances are completely removed. Consequently, many more approximate interproton distance restraints can be obtained from the NOE data than was heretofore possible, thereby expanding the horizons of three-dimensional structure determination by NMR to larger proteins.
Identification and preliminary characterization of a sperm-binding protein in normal human semen (1985)

In the seminal plasma of normal men a protein, immunologically related to a major protein (RSV-IV) secreted from the rat seminal vesicle epithelium, was detected by competition with RSV-IV in a specific radioimmunoassay using a rabbit anti RSV-IV antiserum. The protein was partly (80%) purified by column chromatography; characterization by gel electrophoresis indicated that the protein is slightly basic and has a molecular weight of 140 000. The protein was present on the surface of human spermatozoa as well as in the ejaculates of azoospermic men and it is believed to be a sperm-binding protein.
Noninvasive determination of the optical properties of adult brain: near-infrared spectroscopy approach (2004)

The basic parameters for physiological measurements provided by near-infrared spectroscopy are the local absorption and scattering coefficients. For the adult human head, they have been difficult to measure noninvasively because of the layered structure of the head. The results of measurements of absorption and reduced scattering coefficients through the forehead on 30 adult volunteers using a multidistance frequency domain method are reported. The optode separation distance ranged from 10 to 80 mm and measurements were recorded at 758 and 830 nm. The measured absorption and reduced scattering coefficients of the forehead were used to evaluate the hemoglobin content in the scalp and brain as well as cerebral oxygen saturation. We found that cerebral oxygenation was relatively narrowly distributed within the subject group (the standard deviation was about 3% for scalp and 6% for brain, respectively), whereas hemoglobin concentrations had a relatively broader distribution. We found that as the optode distance increased, the absorption coefficients increased and the scattering coefficients decreased, retrieving the optical values of scalp and brain for shorter and longer optode distances, respectively. We present the transition curves of the absorption and reduced scattering coefficients as functions of the optode distance. In order to verify the values for each layer, a comparison between the experimental data and a prediction based on the two-layer model of the adult head was carried out. The thicknesses of scalp and skull for the two-layer model were obtained by magnetic resonance imaging of a subject's head. The optical parameters obtained from the two-layer model agreed very well with those measured by the multidistance method.
The extracellular matrix as a cell survival factor. (1993)

Programmed cell death (PCD) or apoptosis is a naturally occurring cell suicide pathway induced in a variety of cell types. In many cases, PCD is induced by the withdrawal of specific hormones or growth factors that function as survival factors. In this study, we have investigated the potential role of the extracellular matrix (ECM) as a cell survival factor. Our results indicate that in the absence of any ECM interactions, human endothelial cells rapidly undergo PCD, as determined by cell morphology, nuclei fragmentation, DNA degradation, protein cross-linking, and the expression of the PCD-specific gene TRPM-2. PCD was blocked by plating cells on an immobilized integrin beta 1 antibody but not by antibodies to either the class I histocompatibility antigen (HLA) or vascular cell adhesion molecule-1 (VCAM-1), suggesting that integrin-mediated signals were required for maintaining cell viability. Treatment of the cells in suspension with the tyrosine phosphatase inhibitor sodium orthovanadate also blocked PCD. When other cell types were examined, some, but not all, underwent rapid cell death when deprived of adhesion to the ECM. These results suggest that in addition to regulating cell growth and differentiation, the ECM also functions as a survival factor for many cell types.
Automatic calibration method using transmitting signals of an adaptive array for TDD systems (2001)

Adaptive arrays are especially effective in reducing cochannel interference and increasing channel capacity in time-division duplex (TDD) mobile communication systems because, ideally, the optimum pattern formed in the receiving mode can be applied to the transmitting pattern. However, since the radiation pattern of the adaptive array is generally formed on a baseband, the amplitude and phase errors must be calibrated between the branches of the array due to individual differences in the radio frequency (RF) devices of the receivers and transmitters. Moreover, since these errors change over time due to temperature variations, real-time calibration is required. This correspondence proposes an automatic calibration method using transmitting signals (ACT) for adaptive base station antennas that is suitable for TDD communication systems, i.e., it enables real-time calibration. The effectiveness of the proposed calibration method is demonstrated using an experimental adaptive array testbed.
Vicinal diol cyclic sulfates. Like epoxides only more reactive (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVicinal diol cyclic sulfates. Like epoxides only more reactiveYun. Gao and K. Barry. SharplessCite this: J. Am. Chem. Soc. 1988, 110, 22, 7538–7539Publication Date (Print):October 1, 1988Publication History Published online1 May 2002Published inissue 1 October 1988https://pubs.acs.org/doi/10.1021/ja00230a045https://doi.org/10.1021/ja00230a045research-articleACS PublicationsRequest reuse permissionsArticle Views5336Altmetric-Citations496LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
ERAP140, a Conserved Tissue-Specific Nuclear Receptor Coactivator (2002)

We report here the identification and characterization of a novel nuclear receptor coactivator, ERAP140. ERAP140 was isolated in a screen for ER alpha-interacting proteins using the ER alpha ligand binding domain as a probe. The ERAP140 protein shares no sequence and has little structural homology with other nuclear receptor cofactors. However, homologues of ERAP140 have been identified in mouse, Drosophila, and Caenorhabditis elegans. The expression of ERAP140 is cell and tissue type specific and is most abundant in the brain, where its expression is restricted to neurons. In addition to interacting with ER alpha, ERAP140 also binds ER beta, TR beta, PPAR gamma, and RAR alpha. ERAP140 interacts with ER alpha via a noncanonical interaction motif. The ER alpha-ERAP140 association can be competed by coactivator NR boxes, indicating ERAP140 binds ER alpha on a surface similar to that of other coactivators. ERAP140 can enhance the transcriptional activities of nuclear receptors with which it interacts. In vivo, ERAP140 is recruited by estrogen-bound ER alpha to the promoter region of endogenous ER alpha target genes. Furthermore, the E(2)-induced recruitment of ERAP140 to the promoter follows a cyclic pattern similar to that of other coactivators. Our results suggest that ERAP140 represents a distinct class of nuclear receptor coactivators that mediates receptor signaling in specific target tissues.
Electrophoretic Patterns of Proteins in the Genus Bifidobacterium and Proposal of Four New Species (1982)

The polyacrylamide gel electrophoretic patterns of soluble cellular proteins from 1,094 strains of bifidobacteria were compared with available deoxyribonu-cleic acid (DNA)-DNA homology data and with the phenotypic and biochemical reactions of these strains. There was excellent correlation between the 25 distinct protein patterns and 24 DNA-DNA homology groups in the genus. Differentiation among species on the basis of common phenotypic properties often was unreliable. Our results demonstrate that the species previously known as “Bifidobacterium eriksonii” is a synonym of Bifidobacterium dentium Scardovi and Crociani; that “Actinomyces parabifidus” is a synonym of Bifidobacterium infantis Reuter; that Bifidobacterium globosum (ex Scardovi, Trovatelli, Crociani, and Sgorbati 1969) sp. nov., nom. rev. (type strain, ATCC 25865) and Bifidobacterium pseudolongum Mitsuoka are closely related but distinct entities; and that Bifidobacterium minimum sp. nov. (type strain, ATCC 27538) and Bifidobacterium subtile sp. nov. (type strain, ATCC 27537) are valid species. Our analyses also indicate that Bifidobacterium coryneforme (ex Scardovi and Trovatelli 1969) sp. nov., nom. rev. (type strain, ATCC 25911) is a valid species. A group of strains intermediate between B. infantis and B. longum, “Bifidobacterium infantis-longum” occurs in calf feces. Identical or nearly identical protein patterns were produced by strains that had 80% or greater DNA homology.
Therapeutic antibodies: their mechanisms of action and the pathological findings they induce in toxicity studies (2015)

Antibodies can swiftly provide therapeutics to target disease-related molecules discovered in genomic research. Antibody engineering techniques have been actively developed and these technological innovations have intensified the development of therapeutic antibodies. From the mid-1990's, a series of therapeutic antibodies were launched that are now being used in clinic. The disease areas that therapeutic antibodies can target have subsequently expanded, and antibodies are currently utilized as pharmaceuticals for cancer, inflammatory disease, organ transplantation, cardiovascular disease, infection, respiratory disease, ophthalmologic disease, and so on. This paper briefly describes the modes of action of therapeutic antibodies. Several non-clinical study results of the pathological changes induced by therapeutic antibodies are also presented to aid the future assessment of the toxic potential of an antibody developed as a therapeutic.
Human phase response curve to a 1 h pulse of bright white light (2012)

The phase resetting response of the human circadian pacemaker to light depends on the timing of exposure and is described by a phase response curve (PRC). The current study aimed to construct a PRC for a 1 h exposure to bright white light (∼8000 lux) and to compare this PRC to a <3 lux dim background light PRC. These data were also compared to a previously completed 6.7 h bright white light PRC and a <15 lux dim background light PRC constructed under similar conditions. Participants were randomized for exposure to 1 h of either bright white light (n=18) or <3 lux dim background light (n=18) scheduled at 1 of 18 circadian phases. Participants completed constant routine (CR) procedures in dim light (<3 lux) before and after the light exposure to assess circadian phase. Phase shifts were calculated as the difference in timing of dim light melatonin onset (DLMO) during pre- and post-stimulus CRs. Exposure to 1 h of bright white light induced a Type 1 PRC with a fitted peak-to-trough amplitude of 2.20 h. No discernible PRC was observed in the <3 lux dim background light PRC. The fitted peak-to-trough amplitude of the 1 h bright light PRC was ∼40% of that for the 6.7 h PRC despite representing only 15% of the light exposure duration, consistent with previous studies showing a non-linear duration–response function for the effects of light on circadian resetting.
A C-arm fluoroscopy-guided progressive cut refinement strategy using a surgical robot (2000)

We describe a new method to cut a precise, high-quality femoral cavity in Revision Total Hip Replacement surgery (RTHR) using a surgical robot and an intra-operative C-arm fluoroscope. With respect to previous approaches, our method contains several new features. (1) We describe a novel checkerboard plate designed to correct the geometric distortion within fluoroscopic images. Unlike previous distortion correction devices, the plate does not completely obscure any part of the image, and the distortion correction algorithm works well even when there are some overlaid objects in the field of view. (2) Also included are a novel corkscrew fiducial object designed to be integrated with the robot end-effector, and a 6D pose estimation algorithm based on the two-dimensional (2D) projection of the corkscrew, used in robot-imager registration and imager co-registration. (3) In addition, we develop a cavity location algorithm, which utilizes image subtraction and 2D anatomy contour registration techniques. (4) Finally, we propose a progressive cut refinement strategy, which progressively improves the robot registration during the procedure. We have conducted several experiments, in both simulated and in vitro environments. The results indicate that our strategy is a promising method for precise orthopedic procedures like total hip replacement.
HydroSense (2009)

Recent work has examined infrastructure-mediated sensing as a practical, low-cost, and unobtrusive approach to sensing human activity in the physical world. This approach is based on the idea that human activities (e.g., running a dishwasher, turning on a reading light, or walking through a doorway) can be sensed by their manifestations in an environment's existing infrastructures (e.g., a home's water, electrical, and HVAC infrastructures). This paper presents HydroSense, a low-cost and easily-installed single-point sensor of pressure within a home's water infrastructure. HydroSense supports both identification of activity at individual water fixtures within a home (e.g., a particular toilet, a kitchen sink, a particular shower) as well as estimation of the amount of water being used at each fixture. We evaluate our approach using data collected in ten homes. Our algorithms successfully identify fixture events with 97.9% aggregate accuracy and can estimate water usage with error rates that are comparable to empirical studies of traditional utility-supplied water meters. Our results both validate our approach and provide a basis for future improvements.
Recovered Pulmonary Vein Conduction as a Dominant Factor for Recurrent Atrial Tachyarrhythmias After Complete Circular Isolation of the Pulmonary Veins (2004)

In patients with recurrent ATa after CCLs, recovered PV conduction is a dominant finding in approximately 80% of patients and can be successfully eliminated by segmental RF ablation. Also, mapping and ablation of non-PV arrhythmias can improve clinical success.
Lane recognition based on location of raised pavement markers (2011)

Lane recognition plays an important role in driver assistance systems. Many Lane recognition methods have been proposed until now. But most methods are used to recognize the traditional painted lines and can't work on the road where there are only raised pavement lane markers such as reflective markers and bots dots. Lane recognition on this kind of roads is a difficult problem especially in the daytime for the raised pavement markers are poorly visible and not reflected. In this paper, a novel solution for lane recognition in this case is presented. Firstly, the dot filters and the OLS templates are proposed for location of raised pavement markers. Then a line fitting method based on Hough transform and clustering is used on the accumulation image of raised pavement markers to detect the implicit lane. The experimental results under various scenes show that the proposed method is effective and can be implemented in real-time.
The addition of polydextrose and galactooligossacharide to formula does not affect barrier function or bacterial translocation in neonatal piglets (2009)

The microbiota of breastfed and formula‐fed infants differ, largely due to the prebiotic activity of oligosaccharides in human milk. While there is interest in supplementing infant formulas with prebiotics, some studies suggest that prebiotics may increase bacterial translocation (BT). Thus, the objective of this study was to determine the impact of polydextrose (PDX) and galactooligossacharide (GOS), on intestinal BT in neonatal piglets. Colostrum‐deprived piglets (n=36) were randomized to receive total enteral nutrition as formula (TEN); TEN+GOS (4 g/L), TEN+PDX (4 g/L) or TEN+GOS+PDX (2 g/L each) for 7 days. An additional group of piglets (n=8) was placed on total parenteral nutrition (TPN) as a positive control. Blood, spleen, liver and mesenteric lymph nodes were cultured for aerobic (MacConkey and Trypic Soy + 5% sheep blood) and anaerobic (Wilkins‐Chalgren, Bacteroides Bile Esculin and Lactobacillus Agars) bacteria. Bacterial culturing demonstrated significant BT of Enterobacteriaceae , fastidious anaerobes and fastidious aerobes in TPN piglets. In contrast, BT was insignificant in TEN regardless of the presence of prebiotics. Ileal and colonic villus height and width were not affected by prebiotic supplementation (p&lt;0.05), but were affected by TPN. Our study shows that the addition of PDX and GOS to infant formula does not adversely impact BT. (Supported by Mead Johnson Nutritionals)
Evidence for platelet-activating factor as a novel mediator in experimental stroke in rabbits. (1990)

Platelet-activating factor is a potent mediator of inflammation, which has untoward effects on cerebrovascular and neural elements. While several investigators have reported attenuation of ischemic damage after treatment with antagonists of platelet-activating factor, no study has proved endogenous production of platelet-activating factor in ischemia of the central nervous system. We hypothesized that endogenous production of platelet-activating factor participates in the early pathologic manifestations of deteriorating stroke. In 12 rabbits, we found tissue levels of platelet-activating factor measured by the release of serotonin from washed platelets to be elevated by approximately 20-fold in spinal cord injured by 25 minutes of ischemia and 2 hours of reperfusion (2.80 +/- 0.98 ng/g) compared with that in normal spinal cord (0.15 +/- 0.06 ng/g, p less than 0.01). Given during ischemia to seven rabbits, 10 mg/kg i.p. of a highly selective and potent antagonist of platelet-activating factor (BN 50739) accentuated the early postischemic hyperemia and prevented the delayed hypoperfusion measured by on-line laser-Doppler flowmetry (-35 +/- 7% of baseline [n = 7] without versus 33 +/- 14% with treatment, p less than 0.01) and the edema formation measured as the increase in tissue water content (4.4 +/- 0.7% without [n = 6] versus 2.1 +/- 0.6% with [n = 7]treatment, p less than 0.05) after 2 hours of reperfusion. This neurochemical and pharmacologic evidence emphasizes a new perspective of ischemia-induced phospholipid degradation and suggests an important role for platelet-activating factor in the early manifestations of stroke.
An in Vivo Model of Somatic Cell Gene Therapy for Human Severe Combined Immunodeficiency (1991)

Deficiency of adenosine deaminase (ADA) results in severe combined immunodeficiency (SCID), a candidate genetic disorder for somatic cell gene therapy. Peripheral blood lymphocytes from patients affected by ADA- SCID were transduced with a retroviral vector for human ADA and injected into immunodeficient mice. Long-term survival of vector-transduced human cells was demonstrated in recipient animals. Expression of vector-derived ADA restored immune functions, as indicated by the presence in reconstituted animals of human immunoglobulin and antigen-specific T cells. Retroviral vector gene transfer, therefore, is necessary and sufficient for development of specific immune functions in vivo and has therapeutic potential to correct this lethal immunodeficiency.
Cadherin‐11 contributes to pulmonary fibrosis: potential role in TGF‐β production and epithelial to mesenchymal transition (2011)

Pulmonary fibrosis, characterized by excess deposition of extracellular matrix by myofibroblasts, is a serious component of chronic lung diseases. Cadherin-11 (CDH11) is increased in wound healing and fibrotic skin. We hypothesized that CDH11 is increased in pulmonary fibrosis and contributes its development. CDH11 expression was assessed in lung tissue from idiopathic pulmonary fibrosis patients. The role of CDH11 in lung fibrosis was determined using the bleomycin model of pulmonary fibrosis, and in vitro analyses were performed on A549 cells during the process of epithelial to mesenchymal transition (EMT). Immunohistochemical studies demonstrated CDH11 expression on fibroblasts, epithelial cells, and alveolar macrophages of patients with pulmonary fibrosis and mice given bleomycin. Interestingly, CDH11-deficient mice had decreased fibrotic endpoints in the bleomycin model of pulmonary fibrosis compared to wild-type mice. Furthermore, anti-CDH11-neutralizing monoclonal antibodies successfully treated established pulmonary fibrosis induced by bleomycin. TGF-β levels were reduced in bronchoalveolar lavage (BAL) fluid, BAL cells, and primary alveolar macrophages from CDH11-deficient mice. Mechanistic studies demonstrated that TGF-β up-regulated CDH11 expression on A549 cells, and inhibition of CDH11 expression using siRNA reduced TGF-β-induced EMT. Together, these results identify CDH11 as a novel therapeutic target for pulmonary fibrosis.
2. Data Structures (1998)

2.1. Overview. In this chapter, we discuss the data structures used in the PLTMG package. We begin with the two data structures used to define the region Ω: the triangulation and the skeleton. Unlike earlier versions of PLTMG, which used a triangle tree data structure for refinement and unrefinement, this version implements all adaptive algorithms using only the triangulation data structure. Triangulation and skeleton data structure definitions are summarized in Table 2.1 and described in detail in Sections 2.2 and 2.3. The arrays IP, RP, and SP contain many scalar parameters, switches, control variables, flags, and pointers, some that must be specified by the user and others that are internally computed but may be of interest to the user. These are described in Section 2.4. Finally, the coefficient functions defining the differential operator and functional ρ in (1.1)–(1.3), and the optional function QXY used by TRIGEN and TRIPLT, are described in Section 2.5.