gebhart/patrepeval huggingface dataset:
Atrial natriuretic factor gene expression in ventricles of rats with spontaneous biventricular hypertrophy. (1988)

A subset of Wistar-Kyoto (WKY) rats that spontaneously develops biventricular hypertrophy (BVH) in response to increased cardiac output was evaluated for ventricular expression of the atrial natriuretic factor (ANF) gene. Normal WKY rats had low levels of left ventricular ANF mRNA and minimally detectable ANF transcripts in the right ventricle. In contrast, BVH rats showed a sixfold greater ANF mRNA concentration in the left ventricle than age-matched WKY controls. BVH right ventricular ANF mRNA levels equaled those found in BVH left ventricles and were dramatically greater than WKY right ventricular controls. Unlike experimental models of hypertrophy, both left and right ventricles significantly increase ANF gene transcripts in the natural development of BVH. The left and right ventricles can concordantly respond to hypertrophy and increase ANF gene transcription.
Nanomaterial integrated microfluidic devices for virus analysis (2015)

Outbreaks of viral infectious diseases are often devastating, causing illness, disability, death and massive economic loss. Viral infectious diseases can spread rapid and affecting a large population. The high mutation rate of viruses are especially challenging for disease control and surveillance. Point-of-care technologies using microfluidic principles and integrated with nanomaterials promise to meet these challenges of future analysis of viral infectious diseases by providing cost-effective and sometimes enabling technologies with extraordinary portability, high sensitivity, and fast processing speed.
Control of Peripheral Nerve Myelination by the ß-Secretase BACE1 (2006)

Although BACE1 (beta-site amyloid precursor protein-cleaving enzyme 1) is essential for the generation of amyloid-b peptide in Alzheimer's disease, its physiological function is unclear. We found that very high levels of BACE1 were expressed at time points when peripheral nerves become myelinated. Deficiency of BACE1 resulted in the accumulation of unprocessed neuregulin 1 (NRG1), an axonally expressed factor required for glial cell development and myelination. BACE1-/- mice displayed hypomyelination of peripheral nerves and aberrant axonal segregation of small-diameter afferent fibers, very similar to that seen in mice with mutations in type III NRG1 or Schwann cell-specific ErbB2 knockouts. Thus, BACE1 is required for myelination and correct bundling of axons by Schwann cells, probably through processing of type III NRG1.
6‐Oxocyclohex‐1‐ene‐1‐carbonyl‐coenzyme A hydrolases from obligately anaerobic bacteria: characterization and identification of its gene as a functional marker for aromatic compounds degrading anaerobes (2008)

In anaerobic bacteria, most aromatic growth substrates are channelled into the benzoyl-coenzyme A (CoA) degradation pathway where the aromatic ring is dearomatized and cleaved into an aliphatic thiol ester. The initial step of this pathway is catalysed by dearomatizing benzoyl-CoA reductases yielding the two electron-reduction product, cyclohexa-1,5-diene-1-carbonyl-CoA, to which water is subsequently added by a hydratase. The next two steps have so far only been studied in facultative anaerobes and comprise the oxidation of the 6-hydroxyl-group to 6-oxocyclohex-1-ene-1-carbonyl-CoA (6-OCH-CoA), the addition of water and hydrolytic ring cleavage yielding 3-hydroxypimelyl-CoA. In this work, two benzoate-induced genes from the obligately anaerobic bacteria, Geobacter metallireducens (bamA(Geo)) and Syntrophus aciditrophicus (bamA(Syn)), were heterologously expressed in Escherichia coli, purified and characterized as 6-OCH-CoA hydrolases. Both enzymes consisted of a single 43 kDa subunit. Some properties of the enzymes are presented and compared with homologues from facultative anaerobes. An alignment of the nucleotide sequences of bamA(Geo) and bamA(Syn) with the corresponding genes from facultative anaerobes identified highly conserved DNA regions, which enabled the discrimination of genes coding for 6-OCH-CoA hydrolases from those coding for related enzymes. A degenerate oligonucleotide primer pair was deduced from conserved regions and applied in polymerase chain reaction reactions. Using these primers, the expected DNA fragment of the 6-OCH-CoA hydrolase genes was specifically amplified from the DNA of nearly all known facultative and obligate anaerobes that use aromatic growth substrates. The only exception was the aromatic compound-degrading Rhodopseudomonas palustris, which uniquely uses a modified benzoyl-CoA degradation pathway. Using the oligonucleotide primers, the expected DNA fragment was also amplified in a toluene-degrading and a m-xylene-degrading enrichment culture demonstrating its potential use in less defined bacterial communities. The gene probe established in this work provides for the first time a general tool for the detection of a central functionality in aromatic compound-degrading anaerobes.
Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels (2005)

Hydrogenases encapsulated in porous polymeric silica gels retain significant levels of hydrogen production activity when compared to hydrogenases in solution using reduced methyl viologen as an electron donor. Encapsulated hydrogenases remain active after storage at room temperature for longer than four weeks and are less sensitive to proteolytic digestion. Nanoscopic confinement of active hydrogenases in solids paves the way for their potential use in hydrogen producing catalytic materials applications.
Alternative splicing of the human IgA Fc receptor CD89 in neutrophils and eosinophils (1996)

Receptors for the Fc portion of IgA (Fc alpha R) trigger important immunological elimination processes against IgA-coated targets. Investigation of human Fc alpha R (CD89) transcripts in neutrophils, eosinophils and a monocyte-like cell line, THP-1, with the use of reverse transcriptase PCR, Northern blotting and RNase protection analysis, has provided evidence in these cell types for at least two distinct transcripts generated by alternative splicing. The cDNAs derived from the two major transcripts of both neutrophils and eosinophils have been cloned and sequenced. For both cell types, the larger clone represents the previously described full-length receptor, whereas the second, shorter, splice variant lacks the entire second, membrane-proximal, Ig-like domain. Stable CHO-K1 transfectants have been obtained for both full-length and truncated variant neutrophil receptors. Whereas the full-length receptor is recognized by a panel of five anti-Fc alpha R monoclonal antibodies (mAbs), the shorter variant is bound weakly by only two of the antibodies, suggesting that the epitopes recognized by the majority of the mAbs lie at least in part in the second Ig-like domain of Fc alpha R. Both full-length and splice variant forms of the receptor bind secretory IgA, but the weak binding to serum IgA seen with the full-length receptor is not evident with the shorter variant. Alternative splicing might therefore serve as a means of diversifying Fc alpha R structure and function.
Recombination Dynamics of Localized Excitons in Cubic Phase InxGa1?xN/GaN Multiple Quantum Wells on 3C-SiC/Si (001) (2002)

Recombination dynamics of localized excitons in strained cubic (c-)InxGa1—xN/c-GaN multiple quantum wells (MQWs) were studied. In contrast to hexagonal (h-)InGaN quantum wells (QWs), low-excitation photoluminescence (PL) peak energy increased moderately with decreasing well thickness L and the PL decay time did not depend on L. The results indicated that piezoelectric field had negligible influence on the transition processes. The time-resolved photoluminescence (TRPL) signal showed a stretched exponential decay up to 300 K, showing that the spontaneous emission is due to the radiative recombination of excitons localized in disordered quantum nanostructures even at 300 K. Decrease in internal quantum efficiency (η), which is related to the increase in quasi-radiative lifetime, with the increase in temperature was explained to be due to reduced localization rate.
Anwendung von N‐Tritylmethionin zur Darstellung von biologisch und synthetisch interessanten Heterocyclen (1988)

Abstract N ‐Tritylmethionin‐Derivate liefern durch Überführung in Methylsulfonium‐Salze und anschließende Einwirkung von Natriumhydrid oder Kalium‐ tert ‐butylat die Heterocyclen N ‐(Trityl‐ L ‐homoserinlacton ( 3 ), ( S )‐ bzw. ( R )‐3‐( N ‐Tritylamino)tetrahydrofuran ( 7a, 7b ), ( S )‐1‐Hydroxy‐3‐( N ‐tritylamino)‐2‐pyrrolidinon ( 14 ), ( S )‐4‐( N ‐Tritylamino)‐1,2‐oxazinan‐3‐on ( 15 ), ( S )‐3‐( N ‐Tritylamino)‐2‐pyrrolidon ( 23 ), ( S )‐1‐ tert ‐Butyloxycarbonylamino‐3‐( N ‐tritylamino)‐2‐pyrrolidon ( 27 ) und ( S )‐2‐Methyl‐4‐( N ‐tritylamino)‐1,2‐oxazinan‐3‐on ( 18 ). Durch Methylierung von 14 und 15 erhält man ( S )‐1‐Methoxy‐3‐( N ‐tritylamino)‐2‐pyrrolidon ( 17 ) bzw. 18 . Die Stereochemie der Verbindungen 3, 7a und 17 wird durch Röntgenstrukturanalyse ermittelt.
Camera calibration method based on bundle adjustment (2009)

Camera calibration is the most important step for stereovision measuring system, which affects the accuracy and stability of the measuring system directly. In order to compute the intrinsic and extrinsic parameters of camera, at least seven feature points should be extracted from the calibration board, and the world coordinates of these points should be consistent with their camera coordinates. In this paper, a special planar board utilizing the circular blobs of different sizes is used, and the correspondence between world and image coordinates of these feature points could be built
automatically. Based on an existing algorithm that is used for single camera calibration, the intrinsic and lens radial
distortion parameters can be computed with several image pairs of the planar board that are captured by two cameras in
different orientations as well as the initial orientation and location of cameras. In order to improve the accuracy of object
reconstruction, the bundle adjustment algorithm is further used to optimize the orientation and location of camera.
Experiment results demonstrate the efficiency of proposed method.
Mobile Biometrics (MoBio): Joint Face and Voice Verification for a Mobile Platform (2012)

The Mobile Biometrics (MoBio) project combines real-time face and voice verification for better security of personal data stored on, or accessi-ble from, a mobile platform. 1
Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods (2004)

The normal microbial flora of the vagina plays an important role in preventing genital and urinary tract infections in women. Thus an accurate understanding of the composition and ecology of the ecosystem is important to understanding the aetiology of these diseases. Common wisdom is that lactobacilli dominate the normal vaginal microflora of post-pubertal women. However, this conclusion is based on methods that require cultivation of microbial populations; an approach that is known to yield a biased and incomplete assessment of microbial community structure. In this study cultivation-independent methods were used to analyse samples collected from the mid-vagina of five normal healthy Caucasian women between the ages of 28 and 44. Total microbial community DNA was isolated following resuspension of microbial cells from vaginal swabs. To identify the constituent numerically dominant populations in each community 16S rRNA gene libraries were prepared following PCR amplification using the 8f and 926r primers. From each library, the DNA sequences of approximately 200 16S rRNA clones were determined and subjected to phylogenetic analyses. The diversity and kinds of organisms that comprise the vaginal microbial community varied among women. Species of Lactobacillus appeared to dominate the communities in four of the five women. However, the community of one woman was dominated by Atopobium sp., whereas a second woman had appreciable numbers of Megasphaera sp., Atopobium sp. and Leptotrichia sp., none of which have previously been shown to be common members of the vaginal ecosystem. Of the women whose communities were dominated by lactobacilli, there were two distinct clusters, each of which consisted of a single species. One class consisted of two women with genetically divergent clones that were related to Lactobacillus crispatus, whereas the second group of two women had clones of Lactobacillus iners that were highly related to a single phylotype. These surprising results suggest that culture-independent methods can provide new insights into the diversity of bacterial species found in the human vagina, and this information could prove to be pivotal in understanding risk factors for various infectious diseases.
Myeloperoxidase: Contribution to the Microbicidal Activity of Intact Leukocytes (1970)

Azide and, to a lesser extent, cyanide inhibit the microbicidal activity of myeloperoxidase and of intact normal leukocytes, but they have little or no effect on peroxidase-negative leukocytes. The contribution of the azide-sensitive (peroxidase-dependent?) systems to the total microbicidal activity of normal leukocytes is considerable. The azide-insensitive antimicrobial systems are more highly developed in peroxidase-negative leukocytes than in normal leukocytes, thus suggesting an adaptation.
Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. (1990)

The expression of two cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3), has been investigated in MLA-144 cells before and after induction with phorbol 12-myristate 13-acetate. We describe an adaptation of the polymerase chain reaction (PCR) for highly accurate quantitation of mRNA or DNA from a small number of cells. Aliquots of the PCR mixture containing cDNA copies of the RNA to be assayed were added to serial dilutions of a competitor DNA fragment that differed from the cDNA of interest by having either a small intron or a mutated internal restriction enzyme site. Therefore, the same primers were used to coamplify the unknown and the competitor. The ratio of products remains constant through the amplification and can be readily quantitated. In unstimulated cells, no GM-CSF or IL-3 mRNA could be detected. However, with appropriate induction, mRNA for both cytokines was detected and quantitated in as few as 200 cells. Competitive PCR was also used to accurately quantitate the copy number of the human GM-CSF gene in normal human cells, in a clonal population of cells from a patient with 5q- syndrome, and in a human-hamster cell line known to have only one copy of the human GM-CSF gene.
Epithelial-mesenchymal transition and its implications for fibrosis (2003)

Epithelial to mesenchymal transition (EMT) is a central mechanism for diversifying the cells found in complex tissues. This dynamic process helps organize the formation of the body plan, and while EMT is well studied in the context of embryonic development, it also plays a role in the genesis of fibroblasts during organ fibrosis in adult tissues. Emerging evidence from studies of renal fibrosis suggests that more than a third of all disease-related fibroblasts originate from tubular epithelia at the site of injury. This review highlights recent advances in the process of EMT signaling in health and disease and how it may be attenuated or reversed by selective cytokines and growth factors.
Decellularized scaffolds as a platform for bioengineered organs (2014)

The search for alternatives to allogeneic organ transplantation continues. Important milestones have been achieved in organ bioengineering with the use of decellularized scaffolds. However, many challenges remain on the way to producing an autologous, fully functional organ that can be transplanted similar to a donor organ.
Isolation of human erythropoietin with monoclonal antibodies. (1984)

Human erythropoietin was isolated from urine of aplastic anemic patients in a high yield with a simple purification procedure using an immunoadsorbent column of monoclonal antibodies and a Sephadex G-100 column.About 6 mg of erythropoietin was isolated from 700 liters of urine and the specific activity was estimated to be 81,600 unitslmg of protein with an in vivo 68Fe incorporation assay method, using starved rats.Activity measurement of the extracts from sliced gels after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the Western blotting technique revealed heterogeneity of the isolated erythropoietin, which is probably caused by variable amounts of carbohydrates attached to the polypeptide chain.Thirty amino acids in the NH2-terminal portion of the isolated hormone were sequenced.Erythropoietin is a sialoglycoprotein which is believed to play an important role in regulating, by stimulating, erythropoiesis.Purification of erythropoietin from the urine of patients with aplastic anemia, using conventional purification procedures, has been reported (1, 2).However, laborious purification procedures with low yields and limited supplies of starting material have prevented us from obtaining amounts of pure erythropoietin suitable for studying its structure, mechanism of action, and metabolism.We have prepared a stable hybridoma clone that secretes monoclonal antibody against human urinary erythropoietin.'We describe here a very simple isolation procedure with a high yield of erythropoietin from human urine by the use of this monoclonal antibody. EXPERIMENTAL PROCEDURESMaterials-Erythropoietin (38,000 units/mg of protein with in vivo assay method) was purified from human urine by a combination of conventional methods, immunoadsorbent columns against contaminants and preparative SDS' polyacrylamide gel electrophoresis as a
MIMO Channel Modeling and the Principle of Maximum Entropy (2005)

We devise theoretical grounds for constructing channel models for multiple-input multiple-output (MIMO) systems based on information-theoretic tools. The paper provides a general method to derive a channel model which is consistent with one's state of knowledge. The framework we give here has already been fruitfully explored with success in the context of Bayesian spectrum analysis and parameter estimation. For each channel model, we conduct an asymptotic analysis (in the number of antennas) of the achievable transmission rate using tools from random matrix theory. A central limit theorem is provided on the asymptotic behavior of the mutual information and validated in the finite case by simulations. The results are useful both in terms of designing a system based on criteria such as quality of service and in optimizing transmissions in multiuser networks.
[Study of antibody against protein in human allografts of decalcified bones]. (1993)

The result of study on antibody against protein in human allograft of decalcified bones was reported in this paper. 50 cases with allografts of decalcified bones were detected for sero-antibodies by indirect method of ELISA. The antigens used were extracted protein from the human decalcified bones. Among them, 32 cases showed positive reaction including complete bone graft healing in 30 cases, incomplete healing in one and failed in one cases success rate 98% The other 18 cases showed negative reaction. Neither the Positivith nor the titer of sero-antibody interfered with the healing of allograft.
Self-Assembled Monolayers of Terminal Alkynes on Gold (2007)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSelf-Assembled Monolayers of Terminal Alkynes on GoldSheng Zhang, Kusum L. Chandra, and Christopher B. GormanView Author Information Department of Chemistry, North Carolina State University, Box 8204, Raleigh, North Carolina 27695-8204 Cite this: J. Am. Chem. Soc. 2007, 129, 16, 4876–4877Publication Date (Web):March 31, 2007Publication History Received20 January 2007Published online31 March 2007Published inissue 1 April 2007https://pubs.acs.org/doi/10.1021/ja0704380https://doi.org/10.1021/ja0704380rapid-communicationACS PublicationsCopyright © 2007 American Chemical SocietyRequest reuse permissionsArticle Views3651Altmetric-Citations98LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Gold,Hydrocarbons,Metal nanoparticles,Molecules,Thickness Get e-Alerts
[Experimental study of recovery force of surface-modified TiNi memory alloy rod]. (2006)

The recovery force of Ti-Nb coated and uncoated TiNi shape memory alloy rods was investigated. The rods were 6.0 mm, 6.5 mm and 7.0 mm in diameter respectively. The mean transition temperature was 33.0 degrees C. The rods were stored at -18 degrees C and pre-bent with a three-point bending fixture, the span was 20. 0 centimeters and the deflections were 5.0 mm, 10.0 mm, 15.0 mm and 20.0 mm, respectively. The rods were then heated in a constant temperature saline solution chamber. The experimental temperature was 37.0 C and 50.0 C respectively. The recovery force was measured in a constant displacement mode on biomaterial test machine. The results showed that the recovery force of the memory alloy rod increased with increasing recovery temperature, rod diameter and deformation of both Ti-Nb coated and uncoated surface. The recovery force of Ti-Nb coated rods of 6.0 and 6.5 millimeter in diameter was lower than the uncoated rods in the same diameter. However, the recovery force of 7.0-mm-diameter rods showed no significant difference between coated and uncoated surface.
Functional polarity is introduced by Dicer processing of short substrate RNAs (2005)

Synthetic RNA duplexes that are substrates for Dicer are potent triggers of RNA interference (RNAi). Blunt 27mer duplexes can be up to 100-fold more potent than traditional 21mer duplexes. Not all 27mer duplexes show increased potency. Evaluation of the products of in vitro dicing reactions using electrospray ionization mass spectrometry reveals that a variety of products can be produced by Dicer cleavage. Use of asymmetric duplexes having a single 2-base 3'-overhang restricts the heterogeneity that results from dicing. Inclusion of DNA residues at the ends of blunt duplexes also limits heterogeneity. Combination of asymmetric 2-base 3'-overhang with 3'-DNA residues on the blunt end result in a duplex form which directs dicing to predictably yield a single primary cleavage product. It is therefore possible to design a 27mer duplex which is processed by Dicer to yield a specific, desired 21mer species. Using this strategy, two different 27mers can be designed that result in the same 21mer after dicing, one where the 3'-overhang resides on the antisense (AS) strand and dicing proceeds to the 'right' ('R') and one where the 3'-overhang resides on the sense (S) strand and dicing proceeds to the 'left' ('L'). Interestingly, the 'R' version of the asymmetric 27mer is generally more potent in reducing target gene levels than the 'L' version 27mer. Strand targeting experiments show asymmetric strand utilization between the two different 27mer forms, with the 'R' form favoring S strand and the 'L' form favoring AS strand silencing. Thus, Dicer processing confers functional polarity within the RNAi pathway.