gebhart/patrepeval huggingface dataset:
Adjuvant-free in vivo targeting. Antigen delivery by alpha2-macroglobulin enhances antibody formation. (1994)

The proteinase "inhibitor" alpha 2-macroglobulin (alpha 2M) is able to entrap and form covalent linkages with diverse proteins during a transient proteinase-activated state. These complexes are rapidly endocytosed after binding to receptors present on macrophages and other cells. We have previously shown that compared to free hen egg lysozyme (HEL), alpha 2M-complexed HEL undergoes enhanced macrophage uptake, processing, and presentation to T hybridoma clones in vitro. Inasmuch as it is not clear whether T hybridoma responses accurately reflect primary immune responses in vivo, we studied antibody production in rabbits using two Ag complexed with either human alpha 2M (H alpha 2M) or a homologous protein purified from rabbit plasma, alpha 1-macroglobulin (R alpha 1M). Pathogen-free NZW rabbits received s.c. injections with adjuvant-free preparations of free HEL or porcine pancreatic elastase (PPE), H alpha 2M-HEL-PPE complexes, R alpha 1M-HEL-PPE complexes, or mixtures of the uncomplexed proteins. Complexing the Ag to alpha 2M resulted in 10 to 500-fold higher IgG titers compared to uncomplexed controls. Injection of Ag complexed to either H alpha 2M or R alpha 1M resulted in levels of anti-HEL IgG comparable to those elicited by emulsification in CFA. Inasmuch as inflammatory proteinases such as neutrophil elastase can initiate covalent complex formation with alpha 2M, we propose that "proteinase-activated" alpha 2M may mediate receptor-enhanced Ag uptake by macrophages, resulting in augmented Ag processing and antibody production.
Dual Magnetic-/Temperature-Responsive Nanoparticles for Microfluidic Separations and Assays (2007)

A stimuli-responsive magnetic nanoparticle system for diagnostic target capture and concentration has been developed for microfluidic lab card settings. Telechelic poly(N-isopropylacrylamide) (PNIPAAm) polymer chains were synthesized with dodecyl tails at one end and a reactive carboxylate at the opposite end by the reversible addition fragmentation transfer technique. These PNIPAAm chains self-associate into nanoscale micelles that were used as dimensional confinements to synthesize the magnetic nanoparticles. The resulting superparamagnetic nanoparticles exhibit a gamma-Fe2O3 core ( approximately 5 nm) with a layer of carboxylate-terminated PNIPAAm chains as a corona on the surface. The carboxylate group was used to functionalize the magnetic nanoparticles with biotin and subsequently with streptavidin. The functionalized magnetic nanoparticles can be reversibly aggregated in solution as the temperature is cycled through the PNIPAAm lower critical solution temperature (LCST). While the magnetophoretic mobility of the individual nanoparticles below the LCST is negligible, the aggregates formed above the LCST are large enough to respond to an applied magnetic field. The magnetic nanoparticles can associate with biotinylated targets as individual particles, and then subsequent application of a combined temperature increase and magnetic field can be used to magnetically separate the aggregated particles onto the poly(ethylene glycol)-modified polydimethylsiloxane channel walls of a microfluidic device. When the magnetic field is turned off and the temperature is reversed, the captured aggregates redisperse into the channel flow stream for further downstream processing. The dual magnetic- and temperature-responsive nanoparticles can thus be used as soluble reagents to capture diagnostic targets at a controlled time point and channel position. They can then be isolated and released after the nanoparticles have captured target molecules, overcoming the problem of low magnetophoretic mobility of the individual particle while retaining the advantages of a high surface to volume ratio and faster diffusive properties during target capture.
Lack of teratological effects in rats exposed to 20 or 60 kHz magnetic fields (2011)

Exposure of rats to MFs during organogenesis did not show significant reproducible teratogenicity under experimental conditions. Present findings do not support the hypothesis that intermediate frequency MF exposure after implantation carries a significant risk for developing mammalian fetuses.
Molecular Genetic Dissection of Mouse Unconventional Myosin-VA: Tail Region Mutations (1998)

Abstract We used an RT-PCR-based sequencing approach to identify the mutations responsible for 17 viable dilute alleles, a mouse-coat-color locus encoding unconventional myosin-VA. Ten of the mutations mapped to the MyoVA tail and are reported here. These mutations represent the first extensive collection of tail mutations reported for any unconventional mammalian myosin. They identify sequences important for tail function and identify domains potentially involved in cargo binding and/or proper folding of the MyoVA tail. Our results also provide support for the notion that different myosin tail isoforms produced by alternative splicing encode important cell-type-specific functions.
Little Red Riding Hood meets critical social theory (2001)

article Little Red Riding Hood meets critical social theory Share on Author: Robert L. Glass Computing Trends Computing TrendsView Profile Authors Info & Claims ACM SIGMIS Database: the DATABASE for Advances in Information SystemsVolume 32Issue 4Fall 2001 pp 10–12https://doi.org/10.1145/506139.506141Online:01 September 2001Publication History 0citation338DownloadsMetricsTotal Citations0Total Downloads338Last 12 Months5Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
A Thermally Stable Pt/Y-Based Metal−Organic Framework:  Exploring the Accessibility of the Metal Centers with Spectroscopic Methods Using H2O, CH3OH, and CH3CN as Probes (2006)

A metal-organic framework (MOF) based on Pt, Y, and 2,2'-bipyridine-5,5'-dicarboxylate (BPDC), stable up to 400 degrees C, has been synthesized and characterized. In this MOF, the Pt centers are coordinated to Cl and the N atoms of the BPDC unit, giving a local environment similar to that found in a series of Pt-organic complexes with catalytic activity toward C-H bond cleavage of alkanes. This new material is a heterogeneous counterpart to the corresponding metal-organic complex. The structure, determined by single-crystal XRD data, is the repetition of three covalently bonded layers. These layers form a block, which is stacking as an (a)(b)(c) sequence along the crystallographic b-axis. Each layer contains the Pt-organic unit, while Y atoms represent the connection between adjacent layers. No covalent connection is present between layer (a) of a block and layer (c) of an adjacent block. EXAFS (BM29 at the ESRF) analysis supports the XRD data. As this MOF crystallizes under hydrothermal conditions, water acts both as solvent and as a direct ligand of Y. Accessibility to the metal centers is demonstrated by reversible water desorption/readsorption, as determined by TPA/TPD, FTIR, UV-vis, EXAFS, and XANES. Importantly, the results show that the as-synthesized material will not suffer a permanent loss in porosity upon solvent removal. In addition to water, methanol, ethanol, and acetonitrile can also access the internal void of the dehydrated phase.
Enhanced nonlinear optical effects in rotationally twinned crystals (1975)

We have produced continuously tunable infrared radiation between 4 and 21 μm by mixing the outputs of a ruby laser and a ruby−pumped dye laser in a single rotationally twinned ZnSe crystal. The peak output power of 5 W at 4 μm is approximately 250 times larger than the power obtained from an untwinned ZnSe crystal of comparable length. This enhancement is attributable to randomly spaced rotational twin planes perpendicular to the direction of propagation. Regularly spaced twins should produce an enhancement proportional to the square of the number of twin planes.
The ongoing war between gatherers and tax planners : tax planning (2006)

Extracted from text ... Moneyweb's TAX BREAKS May 2006 3 
TAX PLANNING 
The ongoing war between 
gatherers and tax planners 
By IAN WILSON 
THE TENSION that always exists 
between tax gatherers and tax planners 
ensures there are healthy 
checks and balances within South 
Africa's tax regime. 
The result of the ongoing 'war' 
between the two combatants has 
been a vigorous development of 
legal principle, in the course of 
which many aspects of the relevant 
legal provisions have been tested in 
the courts. 
Without the intervention of 
planners, the real effect and extent 
of the law is not easily established. 
Tax planners, in effect, operate ..
Two-dimensional simulation of local oxidation of silicon: calibrated viscoelastic flow analysis (1996)

Local Oxidation of Silicon (LOCOS) remains the common isolation technology for mass-production of integrated circuits. The work reported in this paper contributes to the improvement of the numerical modeling of the LOCOS process. A physical two-dimensional (2-D) modeling of the thermal oxidation of silicon has been developed based on the explicit treatment of the reaction expansion. The originality of this modeling is to propose a general solution taking into account of the silicon deformation, incorporating the viscoelastic behaviour of oxide and nitride and, particularly, giving a complete calibration of the stress-dependent parameters. The prediction capabilities are demonstrated by the calculations of oxide shapes and oxidation-induced stresses in a silicon substrate for very advanced isolation techniques.
Electrocatalytic Enhancement of Methanol Oxidation at Pt−WOx Nanophase Electrodes and In-Situ Observation of Hydrogen Spillover Using Electrochromism (2003)

A Pt−WOx nanophase electrode showed a considerably more enhanced electrocatalytic activity than Pt itself for methanol oxidation and exactly the reverse change in optical signal intensity with respect to electrochemical cell potential, compared with an electrochromic WOx electrode. This provides evidence for hydrogen spillover from Pt to WOx. Consequently, it was possible to directly observe the in-situ hydrogen transfer from platinum to tungsten oxide in a fuel cell electrode using electrochromism. The transfer of hydrogen ions, produced on the platinum during the electrooxidation of methanol, to the tungsten oxide ensures that the active reaction sites on the platinum remain clean, thus enhancing the electrooxidation current density.
NLRP3 (Nucleotide Oligomerization Domain–Like Receptor Family, Pyrin Domain Containing 3)–Caspase-1 Inflammasome Degrades Contractile Proteins (2017)

Inflammasome-caspase-1-mediated degradation of SMC contractile proteins may contribute to aortic biomechanical dysfunction and AAD development. This cascade may be a therapeutic target in AAD formation. In addition, glyburide may have protective effects against AAD development.
OMICS in assisted reproduction: possibilities and pitfalls (2010)

A key step in assisted reproduction is the assessment of oocyte and embryo developmental potential in order to determine the embryo(s) most likely to result in pregnancy. Currently used embryo assessment strategies are largely based on embryo morphology and cleavage rate. Although these systems have been successful in improving pregnancy rates and reducing multiple gestations, their precision is still insufficient. Therefore, development of an objective, accurate, fast and affordable test that can aid in the assessment of oocyte and embryo developmental potential is a significant aim of reproductive medicine. Recently, global assessment strategies involving genomic, transcriptomic, proteomic or metabolomic profiling of oocytes, granulosa or cumulus cells, embryos or culture media have been applied to assisted reproduction. These technologies are at different stages of development and present unique advantages as well as limitations.
A Review of the State-of-the-Art for the Design of Self-Acting Gas-Lubricated Bearings (1969)

This is a review of selected publications over the past eight years, dealing with the analysis and design of self-acting, gas-lubricated bearings. An effort has been made to evaluate the advances made both in the understanding of the basic phenomena involved and in the technical analytical ability to deal with them. Whenever possible, design procedures have been referenced and illustrated.
The Physical Reality of Space and Surface Waves in the Radiation Field of Radio Antennas (1937)

Evidence is presented which indicates that, notwithstanding the change in sign made by Sommerfeld in his 1926 paper on radio wave propagation, the radiation field of a vertical electric dipole may be separated into space and surface wave components. Sommerfeld's original concepts as to the characteristics of two such waves in radio transmission are largely substantiated. It is shown that a space and a surface wave are generated by a simple vertical dipole antenna at the surface of the earth and that this surface wave has the same wave tilt as the Sommerfeld surface wave. Evidence is given which would indicate that this surface wave travels around the curve of the earth in much the same manner as a guided wire wave travels around a bend on a wire. In the appendix formulas are given for the space and surface waves in the radiation fields of a horizontal electric dipole and of horizontal and vertical magnetic dipoles.
COOH terminus of membrane IgM is essential for an antigen-specific induction of some but not all early activation events in mature B cells. (1991)

Transfectants of mature B cell lines that bind phosphorylcholine were made in order to understand the role of the COOH terminus of the mu chain of membrane IgM (mIgM) in generation of antigen-specific signals. A chimeric receptor (I-A alpha tail) was constructed by replacing 40 amino acids from the mu COOH terminus with that of major histocompatibility complex class II I-A alpha chain. The effect of wild-type and chimeric tails were studied on representative immediate-early antigen-specific signals. The I-A alpha tail hybrid, but not the wild-type receptor, was defective in antigen-driven Ca2+ mobilization, although it could effectively endocytose ligand-receptor complexes. Signal(s) transduced through the wild-type receptor led to transient induction of selected immediate-early gene messages (Egr-1, c-fos, Jun) above basal levels. However, the signal(s) generated after crosslinking of the I-A alpha tail receptor either showed no effect (c-fos) or actually repressed basal level expression of Egr-1 and Jun. Thus, we have established that receptor-mediated endocytosis can be distinguished from other early events associated with B cell activation, based on their differential dependence upon the structural fidelity of the COOH-terminal sequence of mIgM.
Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat (2007)

The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO -is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with a semiquantitative system (0, 1, 2 or 3 crosses) in JG, ventral Bowman glands (VBG), dorsal Bowman glands (DBG), sensitive chemoreceptory neuroepithelium (Ne), receptor-free epithelium (RFE) and nasal septum respiratory epithelium (RE) histological slides of male rats at 5, 15, 25 and 35 days of age. The VBG and JG were intensely positive to PAS reaction since 5 days of age, and weakly positive to Alcyan blue reaction (AB) since 15 days of age, but not at 5 days of age. Both JG and VBG were strongly positive to androgen receptor immunolocalization at all ages studied (15, 25 and 35 days of age). DBG were always negative to both PAS and AB reactions. The NE and the RFE and nasal mucosa luminar epithelium were negative. To our knowledge, this is the first report of androgen receptor (AR) immunolocalization in the vomeronasal organ and the nasal septum mucosa. The present results also suggest an influence of androgens on the mechanism of pheromones contacting with the Ne of the VNO through regulation of the Jacobson glands secretion.
Synthesis of Artificial Opals with Uniform Mesoporous Silica Spheres (2004)

Abstract The mesoporous silica submicron-spheres (80–200 nm) with a small dispersion in diameter (&amp;lt; 10%) as the building blocks of the artificial mesoporous silica opals have been synthesized using quaternary ammonium surfactant as template in an aqueous solution of suitable polarity at near neutral condition of pH = 7.0–9.0.
Probing transmembrane mechanical coupling and cytomechanics using magnetic twisting cytometry (1995)

We recently developed a magnetic twisting cytometry technique that allows us to apply controlled mechanical stresses to specific cell surface receptors using ligand-coated ferromagnetic microbeads and to simultaneously measure the mechanical response in living cells. Using this technique, we have previously shown the following: (i) beta 1 integrin receptors mediate mechanical force transfer across the cell surface and to the cytoskeleton, whereas other transmembrane receptors (e.g., scavenger receptors) do not; (ii) cytoskeletal stiffness increases in direct proportion to the level of stress applied to integrins; and (iii) the slope of this linear stiffening response differs depending on the shape of the cell. We now show that different integrins (beta 1, alpha V beta 3, alpha V, alpha 5, alpha 2) and other transmembrane receptors (scavenger receptor, platelet endothelial cell adhesion molecule) differ in their ability to mediate force transfer across the cell surface. In addition, the linear stiffening behavior previously observed in endothelial cells was found to be shared by other cell types. Finally, we demonstrate that dynamic changes in cell shape that occur during both cell spreading and retraction are accompanied by coordinate changes in cytoskeletal stiffness. Taken together, these results suggest that the magnetic twisting cytometry technique may be a powerful and versatile tool for studies analyzing the molecular basis of transmembrane mechanical coupling to the cytoskeleton as well as dynamic relations between changes in cytoskeletal structure and alterations in cell form and function.
Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. (1995)

CD6 is a 130-kD glycoprotein expressed on the surface of thymocytes and peripheral blood T cells that is involved in TCR-mediated T cell activation. In thymus, CD6 mediates interactions between thymocytes and thymic epithelial (TE) cells. In indirect immunofluorescence assays, a recombinant CD6-immunoglobulin fusion protein (CD6-Rg) bound to cultured human TE cells and to thymic fibroblasts. CD6-Rg binding to TF and TE cells was trypsin sensitive, and 54 +/- 4% of binding was divalent cation dependent. By screening the blind panel of 479 monoclonal antibodies (mAbs) from the 5th International Workshop on Human Leukocyte Differentiation Antigens for expression on human TE cells and for the ability to block CD6-Rg binding to TE cells, we found one mAb (J4-81) that significantly inhibited the binding of CD6-Rg to TE cells (60 +/- 7% inhibition). A second mAb to the surface antigen identified by mAb J4-81, J3-119, enhanced the binding of CD6-Rg to TE cells by 48 +/- 5%. Using covalent cross-linking and trypsin digestion, we found that mAb J4-81 and CD6-Rg both bound to the same 100-kD glycoprotein (CD6L-100) on the surface of TE cells. These data demonstrate that a 100-kD glycoprotein on TE cells detected by mAb J4-81 is a ligand for CD6.
Pediatric pulmonology 2020 year in review: Rare and diffuse lung disease (2021)

Pediatric Pulmonology publishes original research, review articles, and case reports on topics related to a wide range of children's respiratory disorders. Here we review some of the most notable manuscripts published in 2020 in this journal on (1) children's interstitial lung disease (chILD), (2) congenital airway and lung anomalies, and (3) primary ciliary dyskinesia and other non-cystic fibrosis bronchiectasis. The articles reviewed are discussed in context with published works from other journals.
Gene Promoter Methylation in Prostate Tumor–Associated Stromal Cells (2006)

The observation of promoter methylation in the non-neoplastic cells of the prostate tumor microenvironment may advance our understanding of prostate cancer development and progression and lead to new diagnostic and prognostic markers and therapeutic targets.
A 2.4-GHz-band 1.8-V operation single-chip Si-CMOS T/R-MMIC front-end with a low insertion loss switch (2001)

This paper describes the design and experimental results of a 1.8-V single-chip CMOS MMIC front-end for 2.4-GHz band short-range wireless communications, such as Bluetooth and wireless LANs. The IC consists of fundamental RF building circuits-a power amplifier (PA), a low-noise amplifier (LNA), and a transmit/receive-antenna switch (SW), including almost all on-chip matching elements. The IC was fabricated using a 0.18-/spl mu/m standard bulk CMOS technology which has no extra processing steps to enhance the RF performances. Two new circuit-design techniques are introduced in the IC in order to minimize the insertion loss of the SW and realize a higher gain for the PA and LNA despite the utilization of the standard bulk CMOS technology. The first is the derivation of an optimum gate width of the SW to minimize the insertion loss based on small-signal equivalent circuit analysis. The other is the revelation of the advantages of interdigitated capacitors (IDCs) over conventional polysilicon to polysilicon capacitors and the successful use of the IDCs in the LNA and PA. The IC achieves the following sufficient characteristics for practical wireless terminals at 2.1 GHz and 1.8 V: a 5-dBm transmit power at a -1-dB gain compression, a 19-dB gain, an 18-mA current for the PA, a 1.5-dB insertion loss, more than 24-dB isolation, an 11-dBm power handling capability for the SW, a 7.5-dB gain, a 4.5-dB noise figure, and an 8-mA current for the LNA.