gebhart/patrepeval huggingface dataset:
Encapsulated multicellular spheroids of rat hepatocytes produce albumin and urea in a spouted bed circulating culture system. (1991)

Multicellular spheroids are spherical cell-aggregates that retain tridimensional architecture and tissue-specific functions. For use of multicellular spheroids of hepatocytes in a bioreactor for hybrid artificial liver support, we studied the effect of encapsulation and circulating culture on their integrity and tissue-specific functions. Multicellular spheroids of rat hepatocytes were encapsulated into microdroplets of calcium alginate gel and were used as a bioreactor in medium circulating in a spouted bed chamber. Approximately 10% of the hepatocytes of an adult rat were entrapped in a bioreactor chamber, connected to a gas exchanger and a medium reservoir. The total bed volume of the system was 250 ml. The pH and DO2 of the hormonally defined circulating medium was maintained constantly. Albumin and urea were produced in a linear fashion for 64 h at the rates of 0.02 micrograms/microgram cell protein/day and 0.15-0.2 ng/micrograms cell protein/day, respectively. Viability and structural stability of the spheroids were well preserved after the culture period. These results indicate that these encapsulated multicellular hepatocyte spheroids will provide a useful bioreactor for the continuous production of albumin, in vitro and also a prototype hybrid artificial liver support.
Wafer process-induced distortion study for x-ray technology (1991)

X-ray synchrotron radiation is a promising source for submicron lithography. For x-ray lithography to be a useful technology, the combined overlay for the mask, stepper, and wafer processes must be less than that required by the product device specification. X-ray lithography uses a 1× proximity printing technique. Unlike n× optical stepper lithography, a lens cannot be varied nor can a reticle be moved to correct for magnification distortions due to wafer processes. Wafer processes (such as film formation) stress the wafer, causing pattern-magnification distortion and wafer warpage. This paper examines the process’ contribution to the overlay tolerance. Several wafer experiments have been performed using simulated 64 Mbit dynamics random access memory (DRAM) processes. Test wafers, etched with periodic ‘‘L’’ image measurement targets, were both hot processed and trench processed. The ‘‘L’’ targets on the wafers were measured using a long-distance xy measurement system and wafer flatness system. The measurements, before and after each process, show the wafer-induced distortion. One of the experiments discussed in this paper is a hot process used for gate oxidation. This rapid-thermal-oxidation-furnace experiment generated less than 2 ppm distortion. Other examples of hot-process distortion and trench-process distortion data are presented.
Recovery of Xylitol from Fermentation of Model Hemicellulose Hydrolysates Using Membrane Technology (2000)

Xylitol can be produced from xylose or hemicellulose hydrolysates by either chemical reduction or microbial fermentation. Current technology for commercial production is based on chemical reduction of xylose or hemicellulose, and xylitol is separated and purified by chromatographic methods. The resultant product is very expensive because of the extensive purification procedures. Microbial production of xylitol is being researched as an alternative method for xylitol production. Apart from the chromatographic separation method and activated carbon treatment, no other separation method has been proposed for the separation of xylitol from the fermentation broth. Membrane separation was proposed as an alternative method for the recovery of xylitol from the fermentation broth because it has the potential for energy savings and higher purity. A membrane separation unit was designed, constructed, tested, and successfully used to separate xylitol from the fermentation broth. Eleven membranes were investigated for xylitol separation from the fermentation broth. A 10,000 nominal molecular weight cutoff (MWCO) polysulfone membrane was found to be the most effective for the separation and recovery of xylitol. The membrane allowed 82.2 to 90.3% of xylitol in the fermentation broth to pass through while retaining 49.2 to 53.6% of the Lowry's method positive material (such as oligopeptides and peptides). Permeate from the 10,000 MWCO membrane was collected and crystallized. Crystals were analyzed by HPLC for xylitol and impurities and determined to have purity up to 90.3%.
The Sour Taste-Modifying Protein (Miraculin), Tyrosinase Inhibitors and Antioxidants from Synsepalum dulcificum (2009)

There are four basic tastes, including sweet, sour, bitter and salty. Any other taste is merely combination of these four. Red berries of Synsepalum dulcificum, which is an evergreen shrub native of tropical West Africa, have a property in modifying taste by switching sour into a sweet taste remarkably, so the berry has been called miracle fruit. Among all taste proteins, there is one in particular - miraculin - which lacks taste completely when absorbed on its own but has the power of modifying a disagreeable taste into a pleasant one. The active miraculin causes citric acid, ascorbic acid, acetic acid and hydrochloric acid which are normally sour to be perceived as sweet after being held in the mouth. In this review, we demonstrated the miracle fruit development and miraculin identification, including glycosylation and protein structure. We also extracted the fruit of S. dulcificum with methanol (MeOH), and the pulp with chloroform (CHCl3), respectively. All obtained extracts were evaluated for their tyrosinase inhibition and free radical scavenging. The anti-tyrosinase effects were to calculate the hydroxylation of L-tyrosine to L-dopa according to in vitro mushroom tyrosinase assay. The antioxidant potential was evaluated using the following in vitro method: scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. This was the first time to reveal these bioactivities from this species plant to date.
A novel sphingosine kinase 1 inhibitor (SKI-5C) induces cell death of Wilms' tumor cells in vitro and in vivo. (2016)

Sphingosine kinase 1 (SphK1) is over-expressed in many cancers and therefore serves as a biomarker for cancer prognosis. SKI-5C is a new SphK1 inhibitor, and until now its molecular function in Wilms' tumor cells remained unknown. Here, using CCK-8 and nude mice experiments we assessed cell growth in Wilms' tumor cell lines (SK-NEP-1 and G401) <i>in vitro</i> and <i>in vivo</i>. We demonstrated that SphK1 is highly expressed in SK-NEP-1 and G401 cells, and through annexin V/propidium iodide staining and flow cytometry analysis, we detected cell apoptosis. Treatment with SKI-5C inhibited proliferation and induced apoptosis of SK-NEP-1 and G401 cells in a dose-dependent manner. Moreover, SKI-5C treatment inhibited the growth of SK-NEP-1 xenograft tumors in nude mice, with few side effects. Our microarray analysis revealed that SKI-5C-treated SK-NEP-1 cells mostly downregulated PRKACA and significantly inhibited phosphorylation of ERK1/2 and NF-κB p65. These results imply that SKI-5C induces apoptosis of SK-NEP-1 cells through the PRKACA/MAPK/NF-κB pathway. While, further research is required to determine the underlying details, these results provide new clues for the molecular mechanism of cell death induced by SKI-5C and suggest that SKI-5C may act as new candidate drug for Wilms' tumor.
A passive pumping method for microfluidic devices (2002)

The surface energy present in a small drop of liquid is used to pump the liquid through a microchannel. The flow rate is determined by the volume of the drop present on the pumping port of the microchannel. A flow rate of 1.25 microL s(-1) is demonstrated using 0.5 microL drops of water. Two other fluid manipulations are demonstrated using the passive pumping method: pumping liquid to a higher gravitational potential energy and creating a plug within a microchannel.
Deletional, mutational, and methylation analyses of CDKN2 (p16/MTS1) in primary and metastatic prostate cancer (1997)

The tumor suppressor gene CDKN2 (p16/MTS1) resides on chromosome 9p21 and encodes a 16 kDa inhibitor of the cyclin-dependent kinases. Inactivation of CDKN2 by homozygous deletion, point mutation, and recently described aberrant methylation in the 5' promoter region may increase progression through the cell cycle in tumors. In this study, we examine the CDKN2 gene for the presence of inactivating alterations in human prostate cancer. Sequence analysis of cell lines revealed no mutation in LNCaP, PC3, and TSU-PR1 and a missense mutation, GAC-->TAC (asp to tyr), in exon 2 of the DU145 cell line at codon 76. No mutations were identified in three primary prostate cancers or in seven lymph node metastases. Loss of heterozygosity (LOH) was analyzed by analysis of microsatellite markers in the vicinity of the CDKN2 gene. LOH was detected in 12 (20%) of 60 primary tumors at one or more loci and in 13 (46%) of 28 metastases. Methylation analysis of the CpG-rich promoter region revealed a dense methylation of CDKN2 in cell lines PC3, PPC1, and TSU-PR1, and this was found to correlate with a lack of mRNA expression by reverse transcription-polymerase chain reaction. A demethylating agent, 5-aza-2'-deoxycytidine, induced reexpression when cells were exposed in vitro. DU145 and LNCaP expressed the CDKN2 transcript and were unmethylated in the promoter region. Three of twenty-four (13%) primary prostate cancers and 1 of 12 metastatic tumors demonstrated promoter methylation. No normal prostate tissues were methylated at the CDKN2 gene promoter. One tumor was found to contain concomitant LOH and promoter methylation indicative of biallelic inactivation. A comprehensive analysis of CDKN2 in prostate cancer reveals that point mutations are infrequent, but gene deletion and methylation combine to inactivate CDKN2 in a subset of tumors. Moreover, alterations in this gene may represent a late event in prostate cancer progression.
An enzyme-linked immunosorbent assay for antibodies to the envelope glycoproteins of divergent strains of HIV-1 (1989)

We have developed an enzyme-linked immunosorbent assay (ELISA) specific for antibodies to the envelope glycoproteins gp120 and gp160 of HIV-1. An antibody to a conserved epitope on gp120 is adsorbed to a solid phase and used to capture gp120 and/or gp160 from solution. This may be purified recombinant protein or in simple, non-denaturing detergent extracts of different strains of HIV-1. Human serum antibodies bound to the captured antigen are subsequently detected with an anti-human antibody conjugated to alkaline phosphatase, and the AMPAK ELISA amplification system (Novo BioLabs, Cambridge, UK). With this procedure, antibodies can be detected that recognize gp120 from a wide range of divergent HIV-1 strains. The ELISA is sufficiently sensitive to detect env antibodies in sera from HIV-positive individuals at dilutions of 1:300,000. No repeatable false-positives were detected in a screen of 250 normal serum samples. Env antibodies were detected in all 37 strongly HIV-positive sera tested, and in four sera that were borderline or weakly positive in commercial ELISA. However, 55 sera positive in commercial ELISA but unconfirmable by Western blot ('ambiguously' positive) did not contain detectable env antibodies.
A Near Optimal Linear Precoding for Multiuser MIMO Throughput Maximization (2008)

This paper considers a linear precoding scheme that achieves near optimal sum rate. While the minimum mean square error (MMSE) precoding provides the better MSE performance at all signal-to-noise ratio (SNR) than the zero forcing (ZF) precoding, its sum rate shows superior performance to ZF precoding at low SNR but inferior performance to ZF precoding at high SNR. From this observation, we first develop a near sum rate optimal linear precoding scheme for the case of single receive antenna per user. The resulting precoding scheme regularizes ZF precoding to maximize the sum rate, resulting in better sum rate performance than both ZF precoding and MMSE precoding at all SNR. The proposed scheme is extended to the case of the multiple receive antennas, and it shows the better sum rate performance than the block diagonalization (BD) scheme at all SNR.
Retinoic Acid Embryopathy (1985)

Retinoic acid, an analogue of vitamin A, is known to be teratogenic in laboratory animals and has recently been implicated in a few clinical case reports. To study the human teratogenicity of this agent, we investigated 154 human pregnancies with fetal exposure to isotretinoin, a retinoid prescribed for severe recalcitrant cystic acne. The outcomes were 95 elective abortions, 26 infants without major malformations, 12 spontaneous abortions, and 21 malformed infants. A subset of 36 of the 154 pregnancies was observed prospectively. The outcomes in this cohort were 8 spontaneous abortions, 23 normal infants, and 5 malformed infants. Exposure to isotretinoin was associated with an unusually high relative risk for a group of selected major malformations (relative risk = 25.6; 95 per cent confidence interval, 11.4 to 57.5). Among the 21 malformed infants we found a characteristic pattern of malformation involving craniofacial, cardiac, thymic, and central nervous system structures. The malformations included microtia/anotia (15 infants), micrognathia (6), cleft palate (3), conotruncal heart defects and aortic-arch abnormalities (8), thymic defects (7), retinal or optic-nerve abnormalities (4), and central nervous system malformations (18). The pattern of malformation closely resembled that produced in animal studies of retinoid teratogenesis. It is possible that a major mechanism of isotretinoin teratogenesis is a deleterious effect on cephalic neural-crest cell activity that results in the observed craniofacial, cardiac, and thymic malformations.
Association between matrix metalloproteomase-9 polymorphism and coronary heart disease (2012)

Objective To investigate the association between the single polymorphisms(SNPs) of MMP-9 gene C1562T,R279Q and coronary heart disease(CHD).Methods 258 patients diagnosed with CHD and 183 healthy contols were selected.The serum levels of MMP-9 were determinated using enzyme-linked immunosorbent assay(ELISA).PCR-RFLP analysis was applied to detect the C1562T and R279Q SNPs of MMP-9 gene.Statistical soft ware SPSS 17.0 was used to analyze the frequency distribution of alleles and genotypes.Results The serum MMP-9 level in CHD group was significantly higher than that in control group(P0.05).The C1562T genotype frequencies of CT+TT genotypes and T allele were 24.4% and 11.8% in CHD group and 13.8% and 7.3% in control group,respectively,and there were significant differences between two groups(P0.05).The R279Q genotype frequencies of AG+GG genotypes and G allele were 60.2% and 41.7% in CHD group and 68.0% and 41.5% in control group,respectively,and there were no significant differences between two groups(P0.05).Conclusion The MMP-9 level in patients with CHD is increased and the polymorphisms in MMP-9 gene C1562T associates with the susceptibility to the CHD,but there is no association between R279Q polymorphism and CHD.
Tamoxifen and 5-fluorouracil in breast cancer: cytotoxic synergism in vitro. (1983)

The cytokinetic and cytotoxic interactions involved in combining tamoxifen, methotrexate, and 5-fluorouracil were studied in two hormone-dependent human breast cancer cell lines, 47-DN and MCF-7. These cells had measurable cytosol and nuclear estrogen receptor and cytosol progesterone receptor. Growth of the MCF-7 cells in medium containing gelding serum was stimulated maximally by addition of 10 pM estradiol. Both MCF-7 and 47-DN cells showed dose-dependent in vitro growth inhibition on exposure to tamoxifen, and toxicity from tamoxifen at concentrations up to 10 microM could be prevented by 1 nM estradiol. After exposure of 47-DN cells to 10 microM tamoxifen, cytosol progesterone and nuclear estrogen receptor levels were still detectable at 30 and 60% of control values. With this same concentration of tamoxifen, 47-DN cells in S phase declined 50% in association with a buildup of G0-1 cells. By clonogenic assay, tamoxifen enhanced 47-DN and MCF-7 cytotoxicity to 5-fluorouracil and 5-fluorouridine, but not to methotrexate alone. When given either concurrently or using a pretreatment-synchronizing schedule, tamoxifen enhanced markedly the growth inhibition of sequentially combined methotrexate and 5-fluorouracil. Isobologram analysis was used to prove that the cytotoxic interaction between tamoxifen and 5-fluorouracil was synergistic.
Precoding and Signal Shaping for Digital Transmission (2002)

Preface. Introduction. Digital Communications via Linear, Distorting Channels. Precoding Schemes. Signal Shaping. Combined Precoding and Signal Shaping. Appendix A: Wirtinger Calculus. Appendix B: Parameters of the Numerical Examples. Appendix C: Introduction to Lattices. Appendix D: Calculation of Shell Frequency Distribution. Appendix E: Precoding for MIMO Channels. Appendix F: List of Symbols, Variables, and Acronyms. Index.
Sequence of porcine circovirus DNA: affinities with plant circoviruses. (1997)

The complete nucleotide sequence (1759 nt) of the ssDNA genome of porcine circovirus (PCV) was determined from a cloned dsDNA replicative form isolated from PCV-infected cells. Sequence analysis detected no significant nucleic acid or protein similarity with another animal circovirus, chicken anaemia virus (CAV) but, surprisingly, the highest protein similarity was obtained between the product of the largest predicted PCV ORF (ORF1; encoding a potential protein of 35.7 kDa) and a putative protein encoded by the plant circovirus banana bunchy top virus (BBTV). High protein similarity was also detected with the other plant circoviruses subterranean clover stunt virus (SCSV) and coconut foliar decay virus (CFDV). This region of protein identity corresponds with the putative plant circovirus replication-associated protein (Rep). The presence of a nonanucleotide sequence at the apex of a potential-stem loop structure, identical to that found in the plant circoviruses CFDV and SCSV and similar (one mismatch) to that found in the plant circovirus BBTV and in the geminiviruses, suggests that rolling-circle replication may operate during PCV DNA replication. These findings show that PCV is unique in that it bridges the gap between animal and plant circoviruses. The taxonomic relationship of PCV with other members of the Circoviridae is discussed.
ChemInform Abstract: CATALYTIC HYDROGENATION OF SOME 2‐ACETAMIDOALDOSE DERIVATIVES (1973)

Abstract Während die Hexenopyranosederivate (Ia) ‐(Ic) beim Versuch der katalytischen Hydrierung unverändert bleiben, werden aus den Verbindungen (IIa) bzw. (IIb) die Produkte (IIIa) bzw. (IIIb) gebildet.
CAMERA POSITIONING AND CALIBRATION TECHNIQUES FOR INTEGRATING TRAFFIC SURVEILLANCE VIDEO SYSTEMS WITH MACHINE-VISION VEHICLE DETECTION DEVICES (2002)

This project addresses two fundamental areas. First, the project evaluates the technical difficulties associated with integrating machine vision vehicle detection with existing VDOT CCTV camera and camera control equipment (such as calibration, repositioning, and data loss due to pan/tilt operations by traffic controllers). Second, the project develops automated techniques to address these issues. The development effort concentrates on creating repositioning and recalibrating techniques with machine vision technologies. This project develops a prototype approach to integrate VDOT CCTV equipment with existing machine vision traffic detection devices. The prototype and the experience gained developing the prototype, will improve VDOT's understanding of machine vision traffic detection technologies and their appropriateness for system integration in traffic management applications; provide solutions to typical problems associated with machine vision systems in traffic control centers; serve as the proving grounds for the development of machine vision integration plans; and potentially increase the return on VDOT investments in CCTV systems
Mdm2 Inhibits the Apoptotic Function of p53 Mainly by Targeting It for Degradation (2000)

The ability of Mdm2 to inhibit the activities of a C-terminal truncated p53 mutant, p53-Delta30, which can bind Mdm2 but is resistant to Mdm2-mediated protein degradation was investigated. The inhibitory function of an Mdm2 mutant, Mdm2-Delta(222-437), which can bind p53 but is defective in targeting p53 for degradation was also studied. We have demonstrated that targeting p53 for degradation is the most effective way for Mdm2 to inhibit the apoptotic function of p53. However, we have also shown that Mdm2 can inhibit the transactivation function of p53 without targeting it for degradation, although Mdm2 releases the transrepression ability of p53 mainly by targeting it for degradation. The ability of Mdm2 to inhibit the apoptotic function of p53 was linked to its ability to inhibit the transrepression but not the transactivation function of p53. Furthermore, we have demonstrated that the transrepression function of p53 was specific to p53-induced apoptosis and was not simply a result of cell death.
Hyperammonemia-mediated autophagy in skeletal muscle contributes to sarcopenia of cirrhosis (2012)

Hyperammonemia and sarcopenia (loss of skeletal muscle) are consistent abnormalities in cirrhosis and portosystemic shunting. We have shown that muscle ubiquitin-proteasome components are not increased with hyperammonemia despite sarcopenia. This suggests that an alternative mechanism of proteolysis contributes to sarcopenia in cirrhosis. We hypothesized that autophagy could be this alternative pathway since we observed increases in classic autophagy markers, increased LC3 lipidation, beclin-1 expression, and p62 degradation in immunoblots of skeletal muscle protein in cirrhotic patients. We observed similar changes in these autophagy markers in the portacaval anastamosis (PCA) rat model. To determine the mechanistic relationship between hyperammonemia and autophagy, we exposed murine C(2)C(12) myotubes to ammonium acetate. Significant increases in LC3 lipidation, beclin-1 expression, and p62 degradation occurred by 1 h, whereas autophagy gene expression (LC3, Atg5, Atg7, beclin-1) increased at 24 h. C(2)C(12) cells stably expressing GFP-LC3 or GFP-mCherry-LC3 constructs showed increased formation of mature autophagosomes supported by electron microscopic studies. Hyperammonemia also increased autophagic flux in mice, as quantified by an in vivo autophagometer. Because hyperammonemia induces nitration of proteins in astrocytes, we quantified global muscle protein nitration in cirrhotic patients, in the PCA rat, and in C(2)C(12) cells treated with ammonium acetate. Increased protein nitration was observed in all of these systems. Furthermore, colocalization of nitrated proteins with GFP-LC3-positive puncta in hyperammonemic C(2)C(12) cells suggested that autophagy is involved in degradation of nitrated proteins. These observations show that increased skeletal muscle autophagy in cirrhosis is mediated by hyperammonemia and may contribute to sarcopenia of cirrhosis.