gebhart/patrepeval huggingface dataset:
Fluticasone Propionate Aqueous Nasal Spray Is Safe and Effective for Children With Seasonal Allergic Rhinitis (1993)

In children as young as 4 years, 100 micrograms of fluticasone propionate aqueous nasal spray given once daily is as effective as 200 micrograms given once daily, the usual adult dose for the treatment of seasonal allergic rhinitis. Both fluticasone propionate dosages were well tolerated and neither dosage appears to interfere with the hypothalamic-pituitary-adrenal axis in children.
Microwave/SPME method to quantify pesticide residues in tomato fruits (2009)

A new analytical method using focused microwave-assisted extraction (FMAE), coupled with solid phase micro-extraction (SPME), has been elaborated to determine 25 pesticides used in tomato cultivation. Microwave energy was used for a fast and controlled heating of solvent to selectively extract compounds. Calibration curves were plotted from blank tomato samples spiked at different concentrations with standards. A linear response was obtained between 10 and 1000 microg/Kg for pyrethroids and between 0.1 and 5000 microg/Kg for other compounds. For all studied substances, the resulting correlation coefficient (r(2)) was greater than 0.99. Limits of detection (LOD) and quantification (LOQ) were measured lower than 8 and 25 microg/Kg, respectively. The relative standard deviation (RSD) was determined below 15% for all pesticides. Field incurred tomato samples were used to validate the new FMAE/SPME method. Observed analysis results by using this technique were in good agreement compared to those obtained by two accredited trading laboratories using traditional methods. Four tomato samples, bought in a local market, were also tested with the FMAE/SPME method.
At least two kinases phosphorylate the MPM-2 epitope during Xenopus oocyte maturation. (1993)

MPM-2 antigens, a discrete set of phosphoproteins that contain similar phosphoepitopes (the MPM-2 epitope), are associated with various mitotically important structures. The central mitotic regulator cdc2 kinase has been proposed to induce M-phase by phosphorylating many proteins which might include the MPM-2 antigens. To clarify the relationship of cdc2 kinase and the MPM-2 antigens, we developed an in vitro assay that enabled us to specifically detect the kinases that phosphorylate the MPM-2 epitope (ME kinases) in crude cell extracts. Two different ME kinase activities were identified in unfertilized Xenopus eggs, neither of which was cdc2 kinase, but both appeared to be activated by the introduction of cdc2 kinase into oocytes or oocyte extract. The two ME kinases differed in molecular size, substrate specificity, peptide components, and MPM-2 reactivity. The larger one, ME kinase-H, phosphorylated several MPM-2 antigens, while the smaller one, ME kinase-L, phosphorylated mainly one. We purified ME kinase-L to near homogeneity by sequential chromatography and showed that it has the characteristics of the 42-kD microtubule-associated protein (MAP) kinase. Our results support the previous finding that MAP kinase is activated during Xenopus oocyte maturation and suggest that MAP kinase may contribute to oocyte maturation induction by phosphorylating one subtype of MPM-2 epitope.
Expression of chicken liver cell adhesion molecule fusion genes in transgenic mice. (1990)

The tissue-specific expression of the chicken liver cell adhesion molecule (L-CAM) was studied by generating transgenic mice. The rat insulin II promoter was fused to a chicken L-CAM cDNA or to chicken genomic L-CAM sequences. Mice carrying the cDNA showed no expression of L-CAM. Mice carrying L-CAM genomic sequences showed expression in the beta cells of the pancreas, suggesting that sequences in introns or in flanking regions are required for expression. Murine L-CAM was undetectable in the beta cells of the pancreas of those transgenic mice expressing chicken L-CAM and thus appeared to be down-regulated, but expression of the mouse protein was not altered at other sites. Chicken L-CAM was also found in extrapancreatic tissues such as skin, kidney, liver, lung, intestine, blood vessels, and the choroid plexus and leptomeninges of the central nervous system. These findings raised the possibility that the chicken L-CAM gene contains cis regulatory elements that interfere with the specificity of a tissue-specific promoter such as the rat insulin promoter. To test this hypothesis, transgenic mice were produced with a construct containing the murine neurofilament promoter fused to genomic chicken L-CAM sequences. Chicken L-CAM was expressed in the brain and spinal cord, where L-CAM is not normally found, but it was also found in some nonneural tissues (kidney, liver, intestine, lung) in which L-CAM is normally expressed. The combined results suggest that tissue-specific cis-acting elements in the chicken L-CAM gene, when combined with heterologous promoters/enhancers, can generate novel patterns of gene expression.
THE EFFECT OF BENTONITIC SLURRY ON DRILLED SHAFTS. FINAL REPORT (1984)

A series of laboratory and field studies were performed to determine the effect of bentonitic slurry on drilled shafts. Suggestions for construction procedures and slurry specifications are made. When such procedures were followed, no adverse effects have resulted. (Author)
Putative Kappa Opioid Heteromers As Targets for Developing Analgesics Free of Adverse Effects (2014)

It is now generally recognized that upon activation by an agonist, β-arrestin associates with G protein-coupled receptors and acts as a scaffold in creating a diverse signaling network that could lead to adverse effects. As an approach to reducing side effects associated with κ opioid agonists, a series of β-naltrexamides 3-10 was synthesized in an effort to selectively target putative κ opioid heteromers without recruiting β-arrestin upon activation. The most potent derivative 3 (INTA) strongly activated KOR-DOR and KOR-MOR heteromers in HEK293 cells. In vivo studies revealed 3 to produce potent antinociception, which, when taken together with antagonism data, was consistent with the activation of both heteromers. 3 was devoid of tolerance, dependence, and showed no aversive effect in the conditioned place preference assay. As immunofluorescence studies indicated no recruitment of β-arrestin2 to membranes in coexpressed KOR-DOR cells, this study suggests that targeting of specific putative heteromers has the potential to identify leads for analgesics devoid of adverse effects.
Example-based super-resolution (2002)

We call methods for achieving high-resolution enlargements of pixel-based images super-resolution algorithms. Many applications in graphics or image processing could benefit from such resolution independence, including image-based rendering (IBR), texture mapping, enlarging consumer photographs, and converting NTSC video content to high-definition television. We built on another training-based super-resolution algorithm and developed a faster and simpler algorithm for one-pass super-resolution. Our algorithm requires only a nearest-neighbor search in the training set for a vector derived from each patch of local image data. This one-pass super-resolution algorithm is a step toward achieving resolution independence in image-based representations. We don't expect perfect resolution independence-even the polygon representation doesn't have that-but increasing the resolution independence of pixel-based representations is an important task for IBR.
A Three-Kilobase Fragment of the Human Robo4 Promoter Directs Cell Type–Specific Expression in Endothelium (2007)

Robo4, a member of the roundabout family, is expressed exclusively in endothelial cells and has been implicated in endothelial cell migration and angiogenesis. Here we report the cloning and characterization of the human Robo4 promoter. The 3-kb 5'-flanking region directs endothelial cell-specific expression in vitro. Deletion and mutation analyses revealed the functional importance of two 12-bp palindromic DNA sequences at -2528 and -2941, 2 SP1 consensus motifs at -42 and -153, and an ETS consensus motif at -119. In electrophoretic mobility shift assays using supershifting antibodies, the SP1 motifs bound SP1 protein, whereas the ETS site bound a heterodimeric member of the ETS family, GA binding protein (GABP). These DNA-protein interactions were confirmed by chromatin immunoprecipitation assays. Transfection of primary human endothelial cells with small interfering RNA against GABP and SP1 resulted in a significant (approximately 50%) reduction in endogenous Robo4 mRNA expression. The 3-kb Robo4 promoter was coupled to LacZ, and the resulting cassette was introduced into the Hprt locus of mice by homologous recombination. Reporter gene activity was observed in the vasculature of adult organs (particularly in microvessels), tumor xenografts, and embryos, where it colocalized with the endothelial cell-specific marker CD31. LacZ mRNA levels in adult tissues and tumors correlated with mRNA levels for endogenous Robo4, CD31, and vascular endothelial cadherin. Moreover, the pattern of reporter gene expression was similar to that observed in mice in which LacZ was knocked into the endogenous Robo4 locus. Together, these data suggest that 3-kb upstream promoter of human Robo4 contains information for cell type-specific expression in the intact endothelium.
Physical unclonable function and true random number generator (2009)

Physical Unclonable Functions (PUF) and True Random Number Generators (TRNG) are two very useful components in secure system design. PUFs can be used to extract chip-unique signatures and volatile secret keys, whereas TRNGs are used for generating random padding bits, initialization vectors and nonces in cryptographic protocols.
Hydrogen Production By Alkaline Membrane Water Electrolysis (2013)

Abstract not Available.
CO liberated from CORM-2 modulates the inflammatory response in the liver of thermally injured mice (2008)

CORM-released CO modulates liver inflammation and significantly protects liver injury in burn mice by inhibiting the expression of iNOS and NO production, down-regulating the expression of pro-inflammatory mediators (TNF-alpha, IL-1beta).
Digital Video and Audio Compression (1997)

From the Publisher:
Here is a fully readable introduction to the basic technologies,infrastructures,costs,and applications for digital audio and video compression. Delivering a concise account of compression's terms,techniques,and tricks in an easy-to-read style,it covers the basic principles underlying digital signal processing and compression; how human beings see and hear; how audio and video are reproduced; all of the existing and emerging compression standards; video and audio compression techniques; and compression and reproduction requirements of different applications,including videoconferencing. 
A concise,readable account of the compression technologies crucial to the New Media age This is a chronicle of the basic technologies,infrastructures,costs,and applications of digital video and audio compression. Delivering a concise account of compression's terms,techniques,standards,and tricks in an easy-to-read style,it fills a huge gap for everyone from engineers to executives. . . and those currently working with digital media as well as those who would like to. No other single source provides such a clear overview of the technologies that are revolutionzing modes and means of communication around the glove. You'll find straight talk about: The basic principles underlying digital signal processing and compression. ; How human beings see and hear,in relation to how audio and video are reproduced. ; All major existing and emerging compression standards; video compression techniques such as differential pulse code modulation,transform coding,and vector quantization. ; Adaptive pulse code modulation and other audio compression techniques. ; Compression and reproduction requirementsof a wide variety of different applications,including videoconferencing. If the fast,creative,and cost-effective transmission of sights and sounds is a subject about which you need to know,Digital Video and Audio Compression is a must read.
Anticardiolipin antibodies in chronic hepatitis C: Implication of hepatitis C virus as the cause of the antiphospholipid syndrom (1996)

Antiphospholipid antibodies are a type of autoantibodies that have been implicated in the occurrence of thrombocytopenia and thrombotic events and have been described in autoimmune disorders and diverse viral diseases. In this study anticardiolipin antibodies (immunoglobulin G [IgG] isotype) were determined in serum from 100 patients with chronic hepatitis C and 52 healthy controls. In addition, hepatitis C virus (HCV) markers (anti-HCV and HCV RNA) were investigated in 73 patients with thrombotic disorders and no clinical evidence of liver disease; of these patients 37 cases tested negatively for anticardiolipin antibodies and 36 positively. Anticardiolipin test was positive more frequently (22%) in the group of patients with chronic hepatitis C than in healthy controls (1.9%; P < .001). Using conditional logistic-regression analysis we found that in hepatitis C patients the presence of thrombocytopenia, portal hypertension and the existence of prior thrombotic episodes were significantly related to positivity for anticardiolipin antibodies (P < .05 in all cases). In patients with no evidence of liver disease and a history of thrombotic events, hepatitis C markers were absent in all cases who tested negatively for anticardiolipin antibodies (n = 37), but were present in 16.7% of those positive for anticardiolipin (n = 36) (P = .01). In conclusion, anticardiolipin antibodies are frequently found in patients with chronic hepatitis C and in these patients they may be implicated in the occurrence of thrombosis and in the development of thrombocytopenia. Occult HCV infection is present in a significant proportion of patients with thrombotic disorders and positive for anticardiolipin (the antiphospholipid syndrome).
Repair of UVA-Induced Elastic Fiber and Collagen Damage by 0.05% Retinaldehyde Cream in an ex vivo Human Skin Model (1999)

It has been shown that retinaldehyde has many of the properties of tretinoin in its biological and beneficial effects on photoaging. We have verified some of these previous observations, especially on dermal connective tissue, by obtaining significant repair of elastic fibers and collagen alteration induced by UVA exposure.
Lipoxygenase-inhibitory action of antiviral polymeric oxidation products of polyphenols. (1991)

Various polymeric oxidation products of polyphenols strongly inhibited the purified lipoxygenase of rabbit reticulocytes, whereas the prostaglandin H synthase of sheep vesicular gland was only weakly inhibited. The oxidized polymeric preparations of caffeic acid, 2,5-dihydroxytoluene and 3,4-dihydroxytoluene were the most potent lipoxygenase inhibitors. Since such preparations are also known to inhibit herpes simplex virus in an early stage of the virus-cell interaction it is proposed that lipoxygenase metabolites are involved in virus-induced changes in the cell metabolism.
Effect of Dietary Omega‐3 Fatty Acids on Tumor‐Associated Macrophages and Prostate Cancer Progression (2016)

These findings underscore the potential of fish oil in modulating the clinical course of human prostate cancer through the immune system. Further preclinical and clinical studies are warranted evaluating fish oil-based therapies for inhibiting the recruitment and function of M1 and M2 tumor infiltrating macrophages. Prostate 76:1293-1302, 2016. © 2016 Wiley Periodicals, Inc.
GGA Proteins Mediate the Recycling Pathway of Memapsin 2 (BACE) (2004)

Memapsin 2 (BACE, beta-secretase) is a membrane-associated aspartic protease that initiates the hydrolysis of beta-amyloid precursor protein (APP) leading to the production of amyloid-beta (A beta) and the progression of Alzheimer disease. Both memapsin 2 and APP are transported from the cell surface to endosomes where APP is cleaved by memapsin 2. We described previously that the cytosolic domain of memapsin 2 contains an acid cluster-dileucine motif (ACDL) that binds the VHS (Vps-27, Hrs, and STAM) domain of Golgi-localized gamma-ear-containing ARF-binding (GGA) proteins (He, X., Zhu, G., Koelsch, G., Rodgers, K. K., Zhang, X. C., and Tang, J. (2003) Biochemistry 42, 12174-12180). Here we report that GGA proteins colocalize in the trans-Golgi network and endosomes with memapsin 2 and a memapsin 2 chimera containing a cytosolic domain of a mannose-6-phosphate receptor. Depleting cellular GGA proteins with RNA interference or mutation of serine 498 to stop the phosphorylation of ACDL resulted in the accumulation of memapsin 2 in early endosomes. A similar change of memapsin 2 localization also was observed when a retromer subunit, VPS26, was depleted. These observations suggest that GGA proteins function with the phosphorylated ACDL in the memapsin 2-recycling pathway from endosomes to trans-Golgi on the way back to the cell surface.
Glycosylation Site Alteration in the Evolution of Influenza A (H1N1) Viruses (2011)

Influenza virus typically alters protein glycosylation in order to escape immune pressure from hosts and hence to facilitate survival in different host environments. In this study, the patterns and conservation of glycosylation sites on HA and NA of influenza A/H1N1 viruses isolated from various hosts at different time periods were systematically analyzed, by employing a new strategy combining genome-based glycosylation site prediction and 3D modeling of glycoprotein structures, for elucidation of the modes and laws of glycosylation site alteration in the evolution of influenza A/H1N1 viruses. The results showed that influenza H1N1 viruses underwent different alterations of protein glycosylation in different hosts. Two alternative modes of glycosylation site alteration were involved in the evolution of human influenza virus: One was an increase in glycosylation site numbers, which mainly occurred with high frequency in the early stages of evolution. The other was a change in the positional conversion of the glycosylation sites, which was the dominating mode with relatively low frequency in the later evolutionary stages. The mechanisms and possibly biological functions of glycosylation site alteration for the evolution of influenza A/H1N1 viruses were also discussed. Importantly, the significant role of positional alteration of glycosylation sites in the host adaptation of influenza virus was elucidated. Although the results still need to be supported by experimental data, the information here may provide some constructive suggestions for research into the glycosylation of influenza viruses as well as even the design of surveillance and the production of viral vaccines.
Cleavage of Oligonucleotides from Solid-Phase Supports Using o-Nitrobenzyl Photochemistry (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCleavage of Oligonucleotides from Solid-Phase Supports Using o-Nitrobenzyl PhotochemistryMarc M. Greenberg and John L. GilmoreCite this: J. Org. Chem. 1994, 59, 4, 746–753Publication Date (Print):February 1, 1994Publication History Published online1 May 2002Published inissue 1 February 1994https://pubs.acs.org/doi/10.1021/jo00083a014https://doi.org/10.1021/jo00083a014research-articleACS PublicationsRequest reuse permissionsArticle Views504Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Automated DNA sequencing using 4-color fluorescent detection of reactions primed with hexamer strings. (1994)

The use of strings of contiguous short primers rather than a single long primer has the potential to greatly diminish the cost, time and management efforts associated with sequencing by primer walking. For maximum impact, this chemistry must be adaptable to current fluorescent automated sequencers, which, while allowing automated acquisition of data, have reduced sensitivity when compared with radioactive-based sequencing. The ability to use hexamer strings on Applied Biosystems DNA sequencers has now been demonstrated for single-stranded templates. Procedures are described that allow sequence to be obtained up to 400 bases from the priming site. Signal strength is sufficient in this region to allow single base resolution and highly accurate automatic base calling to be performed by the sequencer. While these conditions can no doubt be further optimized, these results show the feasibility of inexpensive primer walking using hexamer string primers on currently available commercial DNA sequencers. This should have a wide range of applications from genome sequencing projects to the sequencing of cDNA clones without the necessity of creating nested deletions or the necessity of spending inordinate amounts of time and money on oligonucleotide synthesis.
Heparin Cofactor IIOslo (1989)

Heparin and dermatan sulfate increase the rate of inhibition of thrombin by heparin cofactor II (HCII) approximately 1000-fold by providing a catalytic template to which both the inhibitor and the proteinase bind. A variant form of HCII that binds heparin but not dermatan sulfate has been described recently in two heterozygous individuals (Andersson, T.R., Larsen, M.L., and Abildgaard, U. (1987) Thromb. Res. 47, 243-248). We have now purified the variant HCII (designated HCIIOslo) from the plasma of ne of these individuals. HCIIOslo or normal HCII (11 nM) was incubated with thrombin (9 nM) for 1 min in the presence of heparin or dermatan sulfate. Fifty percent inhibition of thrombin occurred at 26 micrograms/ml dermatan sulfate with normal HCII and greater than 1600 micrograms/ml dermatan sulfate with HCIIOslo. In contrast, inhibition of thrombin occurred at a similar concentration of heparin (1.0-1.5 micrograms/ml) with both inhibitors. To identify the mutation in HCIIOslo, DNA fragments encoding the N-terminal 220 amino acid residues of HCII were amplified from leukocyte DNA by the Taq DNA polymerase chain reaction and both alleles were cloned. A point mutation (G----A) resulting in substitution of His for Arg-189 was found in one allele. The same mutation was constructed in the cDNA of native HCII by oligonucleotide-directed mutagenesis and expressed in Escherichia coli. The recombinant HCIIHis-189 reacted with thrombin in the presence of heparin but not dermatan sulfate, confirming that this mutation is responsible for the functional abnormality in HCIIOslo.
Face‐Selective Nucleation of Calcite on Self‐Assembled Monolayers of Alkanethiols: Effect of the Parity of the Alkyl Chain (2003)

In-line for promotion: A study of self-assembled monolayers of sulfanylalkanoic acids containing odd and even numbers of methylene groups supported on gold and silver has demonstrated that the crystallographic direction of nucleated calcite crystals is controlled by the orientation of the functional groups in the templating surface (see picture).