gebhart/patrepeval huggingface dataset:
JAK2 (V617F) Mutation Status and Neutrophil Apoptotic Resistance in Myelofibrosis with Myeloid Metaplasia (MMM): Correlation and Potential Identification through Phosphorylation Status of STAT3. (2005)

Abstract Background: We have previously described a resistance to the normal process of apoptosis in neutrophils of patients with myelofibrosis with myeloid metaplasia (MMM) (Blood2003;102:11). Most recently, an activating mutation of JAK2 (V617F) has been described in approximately half of the patients with MMM as well as in variable proportion of patients with other myeloproliferative disorders (MPD). In the current study, we investigated the correlation between JAK2 V617F mutation status and neutrophil apoptosis in MMM. Methods: Neutrophils were isolated by density centrifugation from patients with MMM, other MPDs, and normal controls and assessed for apoptosis at baseline and after 24 hours in culture (IMDM with 20% sterilized fetal calf serum to simulate spontaneous apoptosis). Apoptosis was quantified using three-color flow cytometry using CD45 (to confirm leukocyte presence), annexin V (AN) (marker of apoptosis; detects aberrant externalization of phosphatidylserine during apoptosis), and propidium iodide (PI) (marker of dead cells). Mutation analysis for JAK2 V617F was performed in DNA derived from the isolated neutrophils using genomic DNA amplified by PCR, or extracted from cytogenetic pellets in archived specimens. Apoptotic rates after 24 hours in culture were correlated between patients and controls for both JAK2 mutation status and clinical parameters. Immunoblotting was performed on a subset of patients for correlation of JAK2 mutation status and downstream phosphorylation of the JAK2 target, STAT3, which transcriptionally activates several antiapoptotic genes. Results: Spontaneous neutrophil apoptosis was significantly decreased in MMM patients (n=50; median % apoptotic cells at 41%) compared to both healthy volunteers (n=9; 66%) and patients with other MPD (n=11; 53%) (p=0.002). Resistance to apoptosis in MMM correlated with both anemia (p=0.01) and the presence of the JAK2 V617F mutation (p=0.01). Furthermore, the specific abnormality was more pronounced in patients with homozygous JAK2 V617F; median % apoptotic cells of 47% for patients with wild-type allele (n=22) vs. 39% for heterozygotes (n=23) vs. 22% for homozygotes (n=5; p=0.008). The JAK2 mutation status did not appear dependent on other peripheral blood or clinical features. Neutrophils from 14 MMM patients were assessed simultaneously for both JAK2 mutation and STAT3 phosphorylation status by immunoblotting. Strong expression of phosphorylation of STAT3 was seen in all 3 homozygotes and 4 of 5 heterozygotes, but only 1 of 6 with wild-type allele (p=0.026). Conclusions: Impaired neutrophil apoptosis in patients with MMM correlates with the functional presence of JAK2 V617F in an allele-dose dependent manner and STAT3 phosphorylation. The current observation supports a pathogenetic role for the specific mutation in sustaining clonal myeloproliferation.
Palmitic acid induces production of proinflammatory cytokine interleukin-8 from hepatocytes (2007)

These data demonstrate for the first time that lipid accumulation in hepatocytes can stimulate IL-8 production, thereby potentially contributing to hepatic inflammation and consequent liver injury.
Genetics: Principles and Analysis (1997)

Genetics : principles and analysis , Genetics : principles and analysis , کتابخانه مرکزی دانشگاه علوم پزشکی تهران
Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans (1992)

The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate synthase, homoisocitrate dehydrogenase, alpha-aminoadipate reductase, saccharopine reductase, and saccharopine dehydrogenase) of the alpha-aminoadipate pathway and glucose-6-phosphate dehydrogenase, a glycolytic enzyme used as a control, were demonstrated in wild-type cells of these organisms. All enzymes were present in Saccharomyces cerevisiae and the pathogenic organisms except C. neoformans 32608 serotype C, which exhibited no saccharopine reductase activity. The levels of enzyme activity varied considerably from strain to strain. Variation among organisms was also observed for the control enzyme. Among the pathogens, C. albicans exhibited much higher homocitrate synthase, homoisocitrate dehydrogenase, and alpha-aminoadipate reductase activities. Seven lysine auxotrophs of C. albicans and one of Candida tropicalis were characterized biochemically to determine the biochemical blocks and gene-enzyme relationships. Growth responses to alpha-aminoadipate- and lysine-supplemented media, accumulation of alpha-aminoadipate semialdehyde, and the lack of enzyme activity revealed that five of the mutants (WA104, WA153, WC7-1-3, WD1-31-2, and A5155) were blocked at the alpha-aminoadipate reductase step, two (STN57 and WD1-3-6) were blocked at the saccharopine dehydrogenase step, and the C. tropicalis mutant (X-16) was blocked at the saccharopine reductase step. The cloned LYS1 gene of C. albicans in the recombinant plasmid YpB1078 complemented saccharopine dehydrogenase (lys1) mutants of S. cerevisiae and C. albicans. The Lys1+ transformed strains exhibited significant saccharopine dehydrogenase activity in comparison with untransformed mutants. The cloned LYS1 gene has been localized on a 1.8-kb HindIII DNA insert of the recombinant plasmid YpB1041RG1. These results established the gene-enzyme relationship in the second half of the alpha-aminoadipate pathway. The presence of this unique pathway in the pathogenic fungi could be useful for their rapid detection and control.
Nonhemolytic abiogenic polymers as antimicrobial peptide mimics (2004)

Cationic, facially amphiphilic poly(m-phenylene ethynylene)s were used to investigate whether or not simple polymers can mimic the essential biological activity of host defense peptides. The minimal inhibitory concentrations for two bacteria, Escherichia coli and Bacillus subtilis, were determined and used as models for gram-negative and gram-positive bacteria. Hemolytic activity showed that selectivity toward bacterial cells can occur by the control of the hydrophobic balance and the molecular weight of the system.
A Prime-Boost Strategy Using Virus-Like Particles Pseudotyped for HCV Proteins Triggers Broadly Neutralizing Antibodies in Macaques (2011)

Chronic hepatitis C virus (HCV) infection, with its cohort of life-threatening complications, affects more than 200 million persons worldwide and has a prevalence of more than 10% in certain countries. Preventive and therapeutic vaccines against HCV are thus much needed. Neutralizing antibodies (NAbs) are the foundation for successful disease prevention for most established vaccines. However, for viruses that cause chronic infection such as HIV or HCV, induction of broad NAbs from recombinant vaccines has remained elusive. We developed a vaccine platform specifically aimed at inducing NAbs based on pseudotyped virus-like particles (VLPs) made with retroviral Gag. We report that VLPs pseudotyped with E2 and/or E1 HCV envelope glycoproteins induced high-titer anti-E2 and/or anti-E1 antibodies, as well as NAbs, in both mouse and macaque. The NAbs, which were raised against HCV 1a, cross-neutralized the five other genotypes tested (1b, 2a, 2b, 4, and 5). Thus, the described VLP platform, which can be pseudotyped with a vast array of virus envelope glycoproteins, represents a new approach to viral vaccine development.
Serum-Free Keloid Fibroblast Cell Culture: An In Vitro Model for the Study of Aberrant Wound Healing (1997)

The purpose of this study was to develop an in vitro serum-free keloid fibroblast model. Keloid formation remains a problem for every surgeon. Prior evaluations of fibroblast characteristics in vitro, especially those of growth factor measurement, have been confounded by the presence of serum-containing tissue culture media. The serum itself contains growth factors, yet has been a "necessary evil" to sustain cell growth. The design of this study is laboratory-based and uses keloid fibroblasts obtained from five patients undergoing facial (ear lobule) keloid removal in a university-affiliated clinic. Keloid fibroblasts were established in primary cell culture and then propagated in a serum-free environment. The main outcome measures included sustained keloid fibroblast growth and viability, which was comparable to serum-based models. The keloid fibroblast cell cultures exhibited logarithmic growth, sustained a high cellular viability, maintained a monolayer, and displayed contact inhibition. Demonstrating model consistency, there was no statistically significant difference between the mean cell counts of the five keloid fibroblast cell lines at each experimental time point. The in vitro growth of keloid fibroblasts in a serum-free model has not been done previous to this study. The results of this study indicate that the proliferative characteristics described are comparable to those of serum-based models. The described model will facilitate the evaluation of potential wound healing modulators, and cellular effects and collagen modifications of laser resurfacing techniques, and may serve as a harvest source for contaminant-free fibroblast autoimplants. Perhaps its greatest utility will be in the evaluation of endogenous and exogenous growth factors.
A pilot cluster randomized controlled trial of structured goal-setting following stroke (2011)

A cluster randomized design is not a practical means of avoiding contamination effects in studies of inpatient rehabilitation goal-setting. Other techniques for coping with contamination effects are necessary.
Nanomaterial-Based Sensors for Detection of Disease by Volatile Organic Compounds (2013)

The importance of developing new diagnostic and detection technologies for the growing number of clinical challenges is rising each year. Here, we present a concise, yet didactic review on a new diagnostics frontier based on the detection of disease-related volatile organic compounds (VOCs) by means of nanomaterial-based sensors. Nanomaterials are ideal for such sensor arrays because they are easily fabricated, chemically versatile and can be integrated into currently available sensing platforms. Following a general introduction, we provide a brief description of the VOC-related diseases concept. Then, we focus on detection of VOC-related diseases by selective and crossreactive sensing approaches, through chemical, optical and mechanical transducers incorporating the most important classes of nanomaterials. Selected examples of the integration of nanomaterials into selective sensors and crossreactive sensor arrays are given. We conclude with a brief discussion on the integration possibilities of different types of nanomaterials into sensor arrays, and the expected outcomes and limitations.
Genetic Polymorphism in the Fibrinolytic System and Endometriosis (2006)

II-2.
Advantages of indium–tin oxide‐coated glass slides in correlative scanning electron microscopy applications of uncoated cultured cells (2009)

A method of direct visualization by correlative scanning electron microscopy (SEM) and fluorescence light microscopy of cell structures of tissue cultured cells grown on conductive glass slides is described. We show that by growing cells on indium-tin oxide (ITO)-coated glass slides, secondary electron (SE) and backscatter electron (BSE) images of uncoated cells can be obtained in high-vacuum SEM without charging artefacts. Interestingly, we observed that BSE imaging is influenced by both accelerating voltage and ITO coating thickness. By combining SE and BSE imaging with fluorescence light microscopy imaging, we were able to reveal detailed features of actin cytoskeletal and mitochondrial structures in mouse embryonic fibroblasts. We propose that the application of ITO glass as a substrate for cell culture can easily be extended and offers new opportunities for correlative light and electron microscopy studies of adherently growing cells.
Tumor necrosis factor is an important mediator of tumor cell killing by human monocytes. (1987)

The mechanism of human peripheral blood monocyte-mediated cytotoxicity for tumor cells was investigated, using the A673 human rhabdomyosarcoma and HT-29 human colon adenocarcinoma lines as target cells. A673 cells were shown to be susceptible to the cytotoxic action of purified recombinant human tumor necrosis factor (TNF). A673 cells were also highly sensitive to the cytotoxic action of peripheral blood monocytes. Clones of A673 cells sensitive and resistant to TNF were isolated and characterized for their sensitivity to monocyte killing. A good correlation was found between the sensitivity of these clones to the cytotoxicity of TNF and their susceptibility to killing by monocytes. A TNF-specific neutralizing monoclonal antibody (MAb) reduced monocyte killing of parental A673 cells and of a TNF-sensitive clone of A673 cells. Inhibition of monocyte killing by this MAb was particularly pronounced at a low effector to target cell ratio. HT-29 cells were relatively resistant to the cytotoxic action of recombinant TNF and to monocyte killing. Treatment of HT-29 cells with recombinant human IFN-gamma increased their susceptibility to both TNF cytotoxicity and monocyte killing. In addition, MAb to TNF inhibited monocyte killing in HT-29 cells sensitized by incubation with IFN-gamma. Our data show that TNF is an important mediator of the cytotoxicity of human monocytes for tumor cells and that IFN-gamma can increase monocyte cytotoxicity by sensitizing target cells to the lytic action of TNF.
Reversible LSD1 Inhibition Interferes with Global EWS/ETS Transcriptional Activity and Impedes Ewing Sarcoma Tumor Growth (2014)

These data support epigenetic modulation with HCI2509 as a therapeutic strategy for Ewing sarcoma, and highlight a critical dual role for LSD1 in the oncogenic transcriptional activity of EWS/ETS proteins.
Doxorubicin-conjugated CuS nanoparticles for efficient synergistic therapy triggered by near-infrared light (2016)

To integrate photothermal therapy (PTT) with chemotherapy for improving anticancer efficiency, we developed a novel and multifunctional doxorubicin (DOX) conjugated copper sulfide nanoparticle (CuS-DOX NP) drug delivery system using hydrazone bonds to conjugate carboxyl-functionalized copper sulfide nanoparticles (CuS NPs) and DOX. On the other hand, the hydrazone bonds could be used for improving the DOX release rate (88.0%) by cleavage in a mildly acidic environment irradiated by 808 nm laser light, which could greatly promote chemo-therapeutic efficacy. Simultaneously, CuS NPs which can absorb near infrared (NIR) light produce a clear thermal effect, giving rise to a synergistic therapeutic effect combined with enhanced chemo-therapy. The DOX-conjugated CuS NPs display an evident in vitro cytotoxicity to HeLa cancer cells under 808 nm light irradiation. High tumor inhibition efficacy has been achieved after 14 day in vivo treatment, performed with intravenous administration of CuS-DOX NPs with 808 nm laser irradiation on H22 tumor-bearing mice. The multifunctional system which was achieved by a facile route should be a potential candidate in the anti-cancer field due to the synergistic therapeutic effect, which is superior to any single approach.
Genomic Alterations of Anaplastic Thyroid Carcinoma Detected by Targeted Massive Parallel Sequencing in a BRAF V600E Mutation-Prevalent Area (2016)

This comprehensive analysis of ATCs using targeted massive parallel sequencing identified several novel mutations in ATCs, such as loss of function mutations of NF2 or KMT2D. Future studies are needed to confirm the role of these novel mutations as independent drivers of ATC development.
Synthesis of Water-Soluble, Aliphatic Phosphines and Their Application to Well-Defined Ruthenium Olefin Metathesis Catalysts (1996)

Water-soluble aliphatic phosphines Cy2P(CH2)2N(CH3)3+Cl- (14), Cy2P[4-(N,N-dimethylpiperidinium)]+Cl- (15), Cy2P(CH2)2SO3-Na+ (16), and CyP[(CH2)2N(CH3)3+Cl-]2 (17) (Cy = cyclohexyl) were prepared from air-stable, borane-protected precursors. Spectroscopic investigations of corresponding Pd(PR3)2Cl2 complexes were used to estimate the steric parameters of these new phosphines. Spectroscopic investigation of Ni(CO)3PR3 complexes of the new phosphines indicated that phosphines bearing quaternary amine functionalities were less electron donating than tricyclohexylphosphine, while the presence of a sulfonate group increased phosphine electron donation. Cationic phosphines 14 and 15 were used to synthesize water-soluble ruthenium carbene complexes of the type (Cy2PR)2Cl2RuCHPh. These complexes initiate the ring-opening metathesis polymerization (ROMP) of functionalized 7-oxanorbornenes in water, methanol, and aqueous emulsions.
二年期護理師（護士）訓練計劃實施成效評價 (2011)

背景：合適的護理人員是提供病人安全照護的關鍵。臨床教育訓練可提升專業知能和技能，進而促進工作效能和品質。目的：本研究旨在探討某醫學中心實施「二年期護理師（護士）訓練計劃」後護理人員之護理能力，以評價本訓練計畫之成效。方法：本研究採量性單組後測設計，使用結構式「護理能力」評量表含六大構面共79題，方便取樣收集某醫學中心97年到職滿三個月至一年之新進護理人員，於98年4月進行第一次自我評量，99年4月對同組留任之94位再次評量。結果：本研究結果在照護、臨床、行政、臨床教學、研究及總護理能力等6項效果指標，會受教育程度、服務科別與到院前年資的影響，在危急病人處理能力則受教育程度與到院前年資之影響。在照護、臨床、行政、臨床教學、危急病人處理、研究及總護理能力等7項效果指標，除研究能力外其他指標皆有提升，並達統計上顯著差異，另外37.3%通過N1及N2之護理能力進階，可顯示本訓練計劃之成效。結論／實務應用：研究結果可作為實施與修訂學習護照及教學方法，並加強護理人員能力訓練之參考，進而提升照護品質。
Optimum Sample Utilization in Secondary Ion Mass Spectrometry (1981)

Abstract Secondary ion mass spectrometry (SIMS) is capable of detecting all elements, most of them with high sensitivity. Since the sample volume is eroded during SIMS analysis, particular attention must be paid to the optimum utilization of the consumed sample material. Post-ionization of the sputtered neutrals by different methods has had only limited success; there are basic problems in post-ionizing the neutrals and then effectively collecting the ions for mass spectrometric analysis. The better alternative is to maximize the ionized fraction of the sputtered particles by a suitable choice of the sputtering conditions. The next step is then effective collection and transmission of the secondary ions through the mass analyzer. Various types of mass analyzer are suitable for SIMS: quadrupoe mass filters, sector field mass spectrometers, dc accelerators, time-of-flight mass spectrometers, and sector field mass spectrographs. The choice depends on the analytical requirements such as trace sensitivity, spatial resolution, mass-resolving power, number of elements of interest and duration of analysis. For optimum performance regarding these parameters a large double-focusing mass spectrograph with on-line simulataneous multi-element detection is required.
Functional Annotation of Class I Lysyl-tRNA Synthetase Phylogeny Indicates a Limited Role for Gene Transfer (2002)

Functional and comparative genomic studies have previously shown that the essential protein lysyl-tRNA synthetase (LysRS) exists in two unrelated forms. Most prokaryotes and all eukaryotes contain a class II LysRS, whereas most archaea and a few bacteria contain a less common class I LysRS. In bacteria the class I LysRS is only found in the alpha-proteobacteria and a scattering of other groups, including the spirochetes, while the class I protein is by far the most common form of LysRS in archaea. To investigate this unusual distribution we functionally annotated a representative phylogenetic sampling of LysRS proteins. Class I LysRS proteins from a variety of bacteria and archaea were characterized in vitro by their ability to recognize Escherichia coli tRNA(Lys) anticodon mutants. Class I LysRS proteins were found to fall into two distinct groups, those that preferentially recognize the third anticodon nucleotide of tRNA(Lys) (U36) and those that recognize both the second and third positions (U35 and U36). Strong recognition of U35 and U36 was confined to the pyrococcus-spirochete grouping within the archaeal branch of the class I LysRS phylogenetic tree, while U36 recognition was seen in other archaea and an example from the alpha-proteobacteria. Together with the corresponding phylogenetic relationships, these results suggest that despite its comparative rarity the distribution of class I LysRS conforms to the canonical archaeal-bacterial division. The only exception, suggested from both functional and phylogenetic data, appears to be the horizontal transfer of class I LysRS from a pyrococcal progenitor to a limited number of bacteria.
Studies on 1-Alkyl-2 (1H)-pyridone Derivatives XXXII. The Friedel-Crafts Reaction of 1-Alkyl-2 (1H)-pyridone Derivatives with Acid Anhydride (1990)

The Friedel-Crafts reactions of N-alkyl-2(1H)-pyridone and its derivatives with acid anhydride were carried out. In the cases of N-benzyl-2(1H)-pyridone(1a) and N-benzyl-2(1H)-pyridone(1b), reaction with benzoic anhydride (2a) gave 5-benzoyl compounds (3a, b) and 3, 5-dibenzoyl compounds (4a, b), respectively, in good yields. Reactions of 1a, b with acetic anhydride (2b) gave only 5-acetyl compounds (5a, b). Reactions of dimethyl-2(1H)-pyridone(6a-d) bearing the methyl group on the pyridone ring with 2a were carried out. Only 6a gave 5-benzoyl-1, 3-dimethyl compound (7) and 6b-d gave no benzoyl compound. Reactions of 6a, b and 6d with 2b gave 5-acetyl compounds (8a, b, d), but reaction of 6c with 2b gave no acetyl compound. The result that reactions of 6b and 6d with 2a gave no benzoyl compound may be attributed to the steric hindrance between one methyl group on the pyridone ring and the reagent. Reactions of thiolactam compounds (thiopyridone, thioquinolone) with 2a gave 5-benzoylpyridone (3c) and 6-benzoylquinolone (11) in poor yields, respectively.
Neuroanatomy Through Clinical Cases (2002)

Introduction to Case Presentations - Neuroanatomy Overview, and Basic Definitions - The Neurologic Exam as a Lesson in Neuroanatomy - Introduction to Clinical Neuroradiology - Brain and Environs: Cranium, Ventricles, and Meninges - Corticospinal Tract and Other Motor Pathways - Somatosensory Pathways - Spinal Nerve Roots - Major Plexuses and Peripheral Nerves - Cerebral Hemispheres and Vascular Supply - Visual System - Brainstem I: Surface Anatomy and Cranial Nerves - Brainstem II: Eye Movements and Pupillary Control - Brainstem III: Internal Structure and Vascular Supply - Cerebellum - Basal Ganglia - Pituary and Hypothalamus - Limbic System - Homeostasis, Olfaction, Memory and Evolution - Higher Order Cerebral Function - Epilogue: A Simple Working Model of the Mind - Index to Cases - Subject Index
Technetium-99m labeling by means of stannous pyrophosphate: application to bleomycin and red blood cells. (1975)

Bojana Kunst, philosopher, dramaturg and contemporary art theorist, writes about “the theatre by other means” performed by BADco., on occasion of their Responsibility for Things Seen spatial intervention and video installation in the Aksioma art gallery. BADco. is a Zagreb-based performance collective formed in 2000 by four choreographers and dancers, two dramaturgs and one philosopher. Their performances – which have been presented throughout Europe and the U.S. – do not follow a linear dramaturgical structure, but create the theatre event as a complex synchronization of actions, shifts, speeches and movements, focusing on a concrete political interest. The choreographies, images, spaces ...