gebhart/patrepeval huggingface dataset:
Serum level of squamous cell carcinoma antigen and tumor size are useful to identify preoperatively patients at high risk of cervical cancer (1994)

Serum squamous cell carcinoma antigen assays and clinical tumor size estimated by colposcopic examination are helpful in identifying patients who require postoperative adjunctive therapy.
The Measurement of Species Diversity (1974)

▪ Abstract The literature on effects of habitat fragmentation on biodiversity is huge. It is also very diverse, with different authors measuring fragmentation in different ways and, as a consequence, drawing different conclusions regarding both the ...Read More
Platelet‐activating factor in bronchoalveolar lavage fluid of patients with adult respiratory distress syndrome (1992)

1. To clarify the role of platelet-activating factor (PAF) in the development of adult respiratory distress syndrome (ARDS), we performed bronchoalveolar lavage (BAL) in 19 patients with ARDS and examined cell populations, albumin concentrations and PAF levels. PAF levels were measured by a newly developed radioimmunoassay. 2. In the BAL fluid of ARDS patients, neutrophil percentages and albumin concentrations markedly increased compared with control subjects. 3. PAF was detected in 14 of 19 patients with ARDS, whereas it did not exist in the control subjects. 4. Furthermore, we investigated the priming effect of recombinant human tumour necrosis factor-alpha (TNF alpha), which is known to be one of the most important mediators in the development of ARDS, on PAF production induced by the calcium ionophore in neutrophils. 5. Pre-incubation with TNF alpha dose-dependently increased both extracellular and intracellular PAF production in neutrophils. 6. These results suggest that PAF might play an important role in the development of ARDS.
Attenuated responses to emotional expressions in women with generalized anxiety disorder (2010)

In contrast with previous research, this study found BOLD signal attenuation in the ventrolateral and medial prefrontal cortex and the anterior cingulate cortex during face emotion processing, consistent with a hypothesis of hypo-responsivity to external emotional stimuli in GAD. These decreases were in areas that have been implicated in emotion and cognition and may reflect an altered balance between internally and externally directed attentional processes.
Vaccines. New approaches to immunological problems. (1992)

Haemophilus b conjugate vaccines recent advances in development of pertussis vaccines cholera vaccines malaria helminths influenza vaccines approaches to immunization against respiratory synctial virus hepatitis B vaccines blueprints for vaccines of the future poliovirus vaccines herpes simplex vaccines rotavirus vaccines flavivirus human immunodeficiency virus recent advances in antitumour vaccines vaccinia virus vectors adenovirus based on expression vectors and recombinant vaccines active immunication strategies using anti-idiotypic antibodies passive immunoprophyiaxis with human monoclonal antibodies immunological adjuvants and their mode of action synthetic peptides.
A ONE-POT PROCEDURE FOR THE PREPARATION OF PHOSPHONIC ACIDS FROM ALKYL HALIDES (1990)

(1990). A ONE-POT PROCEDURE FOR THE PREPARATION OF PHOSPHONIC ACIDS FROM ALKYL HALIDES. Organic Preparations and Procedures International: Vol. 22, No. 2, pp. 209-213.
Cleavage by RNase P of Gene N mRNA Reduces Bacteriophage Burst Size (1996)

RNase P, an enzyme essential for tRNA biosynthesis, can be directed to cleave any RNA when the target RNA is in a complex with a short, complementary oligonucleotide called an external guide sequence (EGS). RNase P from Escherichia coli can cleave phage lambda N mRNA in vitro or in vivo when the mRNA is in a complex with an EGS. The EGS can either be separate from or covalently linked to M1 RNA, the catalytic RNA subunit of RNase P. The requirement for Mg2+ in the reaction in vitro is lower when the EGS is covalently linked to M1 RNA. Substrates made of DNA can also be cleaved by RNase P in vitro in complexes with RNA EGSs. When either kind of EGS construct is used in vivo, burst size of phage lambda is reduced by > or = 40%. Reduction in burst size depends on efficient expression of the EGS constructs. The product of phage lambda gene N appears to function in a stoichiometric fashion.
Chromosome Conformation Capture of All 13 Genomic Loci in the Transcriptional Regulation of the Multisubunit Bigenomic Cytochrome c Oxidase in Neurons (2009)

Cytochrome c oxidase (COX) is the terminal enzyme of the electron transport chain composed of 13 subunits; three are mitochondria-encoded, and 10 are nucleus-inscribed on nine different chromosomes within the mammalian genome. The transcriptional regulation of such a multisubunit, multichromosomal, and bigenomic enzyme is mechanistically challenging. Transcription factories have been proposed as one mechanism by which genes from different genomic loci congregate to transcribe functionally related genes, and chromosome conformation capture (3C) is a means by which such interactions can be revealed. Thus far, however, only loci from the same chromosome or at most two chromosomes have been co-localized by 3C. The present study used 3C to test our hypothesis that not only the 10 genomic loci from nine chromosomes encoding the 10 nuclear subunits of COX, but also genes from three chromosomes encoding mitochondrial transcription factors A and B (Tfam, Tfb1m, and Tfb2m) critical for the transcription of the three mitochondria-encoded COX subunit genes all occupy common intranuclear sites in the murine neuronal nuclei. The pairing of various COX subunit genes and Tf genes indicates that interactions are present among all of them. On the other hand, genes for a non-mitochondrial protein (calreticulin) as well as a mitochondrial enzyme (citrate synthase) did not interact with COX genes. Furthermore, interactions between COX subunit and Tf genes were up-regulated by depolarizing stimulation and down-regulated by impulse blockade in primary neurons. Thus, a viable mechanism is in place for a synchronized, coordinated transcriptional regulation of this multisubunit, bigenomic COX enzyme in neurons.
Interaction of Poly(amidoamine) Dendrimers with Supported Lipid Bilayers and Cells:  Hole Formation and the Relation to Transport (2004)

We have investigated poly(amidoamine) (PAMAM) dendrimer interactions with supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayers and KB and Rat2 cell membranes using atomic force microscopy (AFM), enzyme assays, flow cell cytometry, and fluorescence microscopy. Amine-terminated generation 7 (G7) PAMAM dendrimers (10-100 nM) were observed to form holes of 15-40 nm in diameter in aqueous, supported lipid bilayers. G5 amine-terminated dendrimers did not initiate hole formation but expanded holes at existing defects. Acetamide-terminated G5 PAMAM dendrimers did not cause hole formation in this concentration range. The interactions between PAMAM dendrimers and cell membranes were studied in vitro using KB and Rat 2 cell lines. Neither G5 amine- nor acetamide-terminated PAMAM dendrimers were cytotoxic up to a 500 nM concentration. However, the dose dependent release of the cytoplasmic proteins lactate dehydrogenase (LDH) and luciferase (Luc) indicated that the presence of the amine-terminated G5 PAMAM dendrimer decreased the integrity of the cell membrane. In contrast, the presence of acetamide-terminated G5 PAMAM dendrimer had little effect on membrane integrity up to a 500 nM concentration. The induction of permeability caused by the amine-terminated dendrimers was not permanent, and leaking of cytosolic enzymes returned to normal levels upon removal of the dendrimers. The mechanism of how PAMAM dendrimers altered cells was investigated using fluorescence microscopy, LDH and Luc assays, and flow cytometry. This study revealed that (1) a hole formation mechanism is consistent with the observations of dendrimer internalization, (2) cytosolic proteins can diffuse out of the cell via these holes, and (3) dye molecules can be detected diffusing into the cell or out of the cell through the same membrane holes. Diffusion of dendrimers through holes is sufficient to explain the uptake of G5 amine-terminated PAMAM dendrimers into cells and is consistent with the lack of uptake of G5 acetamide-terminated PAMAM dendrimers.
In situdetection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS (2007)

The method presented combines the high discriminatory power of short oligonucleotide probes with the impressive amplification power and selectivity of the rolling circle reaction, providing excellent signal to noise ratios in combination with exact target localization due to the target primed reaction. Furthermore, the procedure is easily multiplexed, allowing visualization of several different RNAs.
[Studies on the chemical constituents from Lobelia chinensis]. (2010)

Compounds 1 -5 and 10 -12 are isolated from this plant for the first time.
Evolution of matrix metalloprotease and tissue inhibitor expression during heart failure progression in the infarcted rat (2000)

MMP/TIMP upregulation evolves over time following infarction in the rat LV. Some MMPs were significantly elevated during the first week post-MI (MMP-13, -2, and -9) and another was not until 16 weeks post-MI (MMP-14). The dissociation between LV MMP/TIMP mRNA and protein levels shows that post-translation processing occurs in the rat heart.
Metabolites of microorganisms. 247. Phenazines from Streptomyces antibioticus, strain TUE 2706. (1988)

From a strain of Streptomyces antibioticus seven yellow phenazines were isolated. The antibacterially most active antibiotic was identified as (-)-saphenamycin, a second one with compound DC-86-Y (saphenic acid). Three compounds were new: Saphenic acid methyl ether, 6-acetylphenazine-1-carboxylic acid and an inseparable mixture of fatty acid esters of saphenic acid. Two simple phenazines were phenazine-1-carboxylic acid (tubermycin B) and unsubstituted phenazine, which was isolated for the first time from a microorganism.
임신부 혈청에서 Insulin - like Growth Factor ( IGF ) 및 IGF-Binding Protein-3 의 특성 (1997)

Background: Pregnancy in human and rodents is associated with dramatic matemal metabolic changes. Insulin-like growth factors(IGFs) are mitogenic peptides that are essential for fetal and maternal tissue growth during pregnancy. They circulate complexed primarily with a serum IGF-binding protein(IGFBP-3) which regulates the availability of the IGFs to their specific target tissues. Methods: To examine the changes of IGFs and IGFB-3 during pregnancy, we measured serum total IGF-I, free IGF-I, IGF-II and IGFBP-3 by using specific radioimmunoassay, immunoradio- metric assay, western ligand blot and western immunoblot. Blood samples were obtained from 88 pregnant women between 6-40 weeks gestation. Results: While serum IGF-I levels increased up to 50% in late pregnancy, serum IGF-II levels remained unchanged. However, serum free IGF-I levels were significantly higher during pregnancy than in nonpregnancy. Western ligand blot analysis revealed that IGFBP-3 in pregnancy serum was significantly decreased at 6 weeks of gestation, continued decreased level until term, and returned to a nonpregnant level by postpartum 10 day. Serum IGFBP-3 profiles in Western immunoblot analysis revealed that 30 kDa fragments of IGFBP-3 were detectable in pregnancy serum but not in nonpregnancy serum. In contrast, serum IGFBP-3 levels using radioimmunoassay was significantly increased in late pregnancy. Conclusions: 1) serum IGF-I was significantly elevated in late pregnancy 2) serum IGF-II was not significantly changed 3) free IGF-I significantly elevated throughout gestation 4) intact IGFBP- 3 was markedly reduced after 6 weeks of gestation. (J Kor Soc Endocrinol 12:376-385, 1997)
Flexible strain sensors with high performance based on metallic glass thin film (2017)

Searching strain sensitive materials for electronic skin is of crucial significance because of the restrictions of current materials such as poor electrical conductivity, large energy consumption, complex manufacturing process, and high cost. Here, we report a flexible strain sensor based on the Zr55Cu30Ni5Al10 metallic glass thin film which we name metallic glass skin. The metallic glass skin, synthesized by ion beam deposition, exhibits piezoresistance effects with a gauge factor of around 2.86, a large detectable strain range (∼1% or 180° bending angle), and good conductivity. Compared to other e-skin materials, the temperature coefficient of resistance of the metallic glass skin is extremely low (9.04 × 10−6 K−1), which is essential for the reduction in thermal drift. In addition, the metallic glass skin exhibits distinct antibacterial behavior desired for medical applications, also excellent reproducibility and repeatability (over 1000 times), nearly perfect linearity, low manufacturing cost, and negligible energy consumption, all of which are required for electronic skin for practical applications.
Social representations of gender and young children's play: A replication (1988)

Forty target children aged 3 to 4 years were observed playing with gender stereotyped toys with both a boy and a girl partner in a replication and extension of an earlier study of toy choice in action and pretend play (Lloyd &amp; Smith, 1985). The findings which were replicated were: (1) in action play girls used girls' toys more and boys used boys' toys more, (2) in pretend play girls used girls' toys more but no differentiation occurred in the use of boys' toys. The absence of pair‐type effects which were found in the earlier study raises questions about socio‐psychological aspects of the replication design.
Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli (2004)

Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.
High-speed and low-power electro-optical DSP coprocessor (2009)

Phase sensitive x-ray imaging extends standard x-ray microscopy techniques by offering up to a thousand times higher sensitivity than absorption-based techniques. If an object is illuminated with a sufficiently coherent beam, phase contrast is achieved by moving the detector downstream from the object. There is a quantitative relationship between the phase shift induced by the object and the recorded intensity. This relationship can be used to retrieve the phase shift induced by the object through the solution of an inverse problem. Since the phase shift can be considered as a projection through the 3D refractive index, the latter can be reconstructed using standard tomographic inversion techniques. However, the determination of the phase shift from the recorded intensity is an ill-posed inverse problem. We investigate the application of Fourier-wavelet regularized deconvolution (ForWaRD) to this problem. The method is evaluated using simulated and experimental data and is shown to increase the quality of reconstructions, in terms of normalized RMS error and compared with standard Tikhonov regularization, at a three times increase in computational cost.
Protective Immune Responses to a Recombinant Adenovirus Type 35 Tuberculosis Vaccine in Two Mouse Strains: CD4 and CD8 T-Cell Epitope Mapping and Role of Gamma Interferon (2007)

There is an urgent need for an efficacious vaccine against tuberculosis (TB). Cellular immune responses are key to an effective protective response against TB. Recombinant adenovirus (rAd) vectors are especially suited to the induction of strong T-cell immunity and thus represent promising vaccine vehicles for the prevention of TB. We have previously reported on rAd vector serotype 35, the serotype of choice due to low preexisting immunity worldwide, which expresses a unique fusion protein of Mycobacterium tuberculosis antigens Ag85A, Ag85B, and TB10.4 (Ad35-TBS). Here, we demonstrate that Ad35-TBS confers protection against M. tuberculosis when administered to mice through either an intranasal or an intramuscular route. Histological evaluation of lung tissue corroborated the protection and, in addition, demonstrated differences between two mouse strains, with diffuse inflammation in BALB/c mice and distinct granuloma formation in C57BL/6 mice. Epitope mapping analysis in these mouse strains showed that the major T-cell epitopes are conserved in the artificial fusion protein, while three novel CD8 peptides were discovered. Using a defined set of T-cell epitopes, we reveal differences between the two mouse strains in the type of protective immune response, demonstrating that different antigen-specific gamma interferon (IFN-gamma)-producing T cells can provide protection against M. tuberculosis challenge. While in BALB/c (H-2(d)) mice, a dominant CD8 T-cell response was detected, in C57BL/6 (H-2(b)) mice, more balanced CD4/CD8 T-cell responses were observed, with a more pronounced CD4 response in the lungs. These results unify conflicting reports on the relative importance of CD4 versus CD8 T-cell responses in protection and emphasize the key role of IFN-gamma.
Cation-anion combination reactions. 23. Solvent effects on rates and equilibria of reactions (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCation-anion combination reactions. 23. Solvent effects on rates and equilibria of reactionsCalvin D. RitchieCite this: J. Am. Chem. Soc. 1983, 105, 11, 3573–3578Publication Date (Print):June 1, 1983Publication History Published online1 May 2002Published inissue 1 June 1983https://pubs.acs.org/doi/10.1021/ja00349a035https://doi.org/10.1021/ja00349a035research-articleACS PublicationsRequest reuse permissionsArticle Views115Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
On the universality of inversion layer mobility in Si MOSFET's: Part I-effects of substrate impurity concentration (1994)

This paper reports the studies of the inversion layer mobility in n- and p-channel Si MOSFET's with a wide range of substrate impurity concentrations (10/sup 15/ to 10/sup 18/ cm/sup -3/). The validity and limitations of the universal relationship between the inversion layer mobility and the effective normal field (E/sub eff/) are examined. It is found that the universality of both the electron and hole mobilities does hold up to 10/sup 18/ cm/sup -3/. The E/sub eff/ dependences of the universal curves are observed to differ between electrons and holes, particularly at lower temperatures. This result means a different influence of surface roughness scattering on the electron and hole transports. On substrates with higher impurity concentrations, the electron and hole mobilities significantly deviate from the universal curves at lower surface carrier concentrations because of Coulomb scattering by the substrate impurity. Also, the deviation caused by the charged centers at the Si/SiO/sub 2/ interface is observed in the mobility of MOSFET's degraded by Fowler-Nordheim electron injection.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
PP.16.15 (2015)

Objective: The objective of this study was to analyses the major risk factors for coronary artery disease (CAD) among patients with ischemic heart disease in the state of Kerala in South India. Design and method: Study was done among randomly selected 496 patients who were admitted in the Department of Cardiology – in a tertiary Cardiac hospital in the state of Kerala in South India during the month of June –December 2012. Patients included for the study were those with Coronary artery disease, either with Acute Coronary Syndrome or with angiographic, ECG or stress test evidence for Ischemic heart disease. Risk factors studied were the conventional risk factors for Coronary artery disease- Hypertension, Diabetes Mellitus, Dyslipidemia, Body Mass index, Smoking and Family History of Coronary artery disease. Data collected from the patients, old medical records, Clinical Examination and Laboratory results of the patients were analyzed for the study. Results: From the study, it was seen that in Keralites-irrespective of gender, diabetes or impaired glucose tolerance (79%) and dyslipidemia (71%) are the major risk factor for Coronary artery disease. Hypertension (39%) and cigarette smoking (24%) were not seen to be a major risk factors for coronary artery disease as only a minority of the study population had hypertension or gives a history of cigarette smoking. 57% of the study population had a family history of coronary artery disease. Among the studied population, 55% of females are with increased BMI, whereas only 16% of males with CAD were with BMI above 30. Conclusions: Among South Indian population in the state of Kerala irrespective of gender, diabetes mellitus and dyslipidemia are the major Risk factor for Coronary artery disease. So early detection of diabetes mellitus and dyslipidemia and proper treatment of both, before developing the end organ damage, play a vital role for the prevention of coronary artery disease.
Generation of an Intensely Potent Anthracycline by a Monoclonal Antibody−β-Galactosidase Conjugate (2005)

The L49 monoclonal antibody against the p97 antigen on melanomas and carcinomas was chemically conjugated to E. coli beta-galactosidase (beta-gal), forming a largely monomeric conjugate with preserved enzymatic activity. The resulting L49-beta-gal conjugate was used to activate (N-[(4"R,S)-4"-hexyloxy-4"-(1'''-O-beta-D-galactopyranosyl)butyl]daunorubicin) (1), a derivative of daunorubicin that has low cytotoxicity and high chemical stability. Addition of the conjugate to the prodrug resulted in an increase in cytotoxicity of approximately 10(5)-fold, a level of activation that is higher than any mAb-enzyme/prodrug combination yet described. Furthermore, the released drug had an IC(50) value of approximately 10 pM, making it significantly more potent than the vast majority of clinically approved anticancer drugs. The potential of this enzyme/prodrug combination for cancer therapy is discussed.