gebhart/patrepeval huggingface dataset:
The histone acetylase PCAF is a nuclear receptor coactivator (1998)

Whereas the histone acetylase PCAF has been suggested to be part of a coactivator complex mediating transcriptional activation by the nuclear hormone receptors, the physical and functional interactions between nuclear receptors and PCAF have remained unclear. Our efforts to clarify these relationships have revealed two novel properties of nuclear receptors. First, we demonstrate that the RXR/RAR heterodimer directly recruits PCAF from mammalian cell extracts in a ligand-dependent manner and that increased expression of PCAF leads to enhanced retinoid-responsive transcription. Second, we demonstrate that, in vitro, PCAF directly associates with the DNA-binding domain of nuclear receptors, independently of p300/CBP binding, therefore defining a novel cofactor interaction surface. Furthermore, our results show that dissociation of corepressors enables ligand-dependent PCAF binding to the receptors. This observation illuminates how a ligand-dependent receptor function can be propagated to regions outside the ligand-binding domain itself. On the basis of these observations, we suggest that PCAF may play a more central role in nuclear receptor function than previously anticipated.
Explanation-based facial motion tracking using a piecewise Bezier volume deformation model (2003)

Capturing real motions from video sequences is a powerful method for automatic building of facial articulation models. In this paper, we propose an explanation-based facial motion tracking algorithm based on a piecewise Bezier volume deformation model (PBVD). The PBVD is a suitable model both for the synthesis and the analysis of facial images. It is linear and independent of the facial mesh structure. With this model, basic facial movements, or action units, are interactively defined. By changing the magnitudes of these action units, animated facial images are generated. The magnitudes of these action units can also be computed from real video sequences using a model-based tracking algorithm. However, in order to customize the articulation model for a particular face, the predefined PBVD action units need to be adaptively modified. In this paper, we first briefly introduce the PBVD model and its application in facial animation. Then a multiresolution PBVD-based motion tracking algorithm is presented. Finally, we describe an explanation-based tracking algorithm that takes the predefined action units as the initial articulation model and adaptively improves them during the tracking process to obtain a more realistic articulation model. Experimental results on PBVD-based animation, model-based tracking, and explanation-based tracking are shown in this paper.
Cobalt Ion Mediated Self-Assembly of Genetically Engineered Bacteriophage for Biomimetic Co−Pt Hybrid Material (2005)

Biological scaffolds are used for the synthesis of inorganic materials due to their ability to self-assemble and nucleate crystal formation. We report the self-assembly of engineered M13 bacteriophage as a template for Co-Pt crystals. A M13 phage library with an octapeptide library on the major coat protein (pVIII) was used for selection of binders to cobalt ions. Fibrous structures with directionally ordered M13 phage were obtained by interaction with cobalt ions. Co-Pt alloys were synthesized on the fibrous scaffold, and their magnetic properties were characterized. The mineralization showed organized nanoparticles on fibrous bundles. This approach using the phage pVIII library allows for genetic selection that both induces assembly of the phage and directs mineralization of the selected inorganic material.
Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding (1989)

Six individual amino acid substitutions at separate positions in the tertiary structure of subtilisin BPN' (EC 3.4.21.14) were found to increase the stability of this enzyme, as judged by differential scanning calorimetry and decreased rates of thermal inactivation. These stabilizing changes, N218S, G169A, Y217K, M50F, Q206C, and N76D, were discovered through the use of five different investigative approaches: (1) random mutagenesis; (2) design of buried hydrophobic side groups; (3) design of electrostatic interactions at Ca2+ binding sites; (4) sequence homology consensus; and (5) serendipity. Individually, the six amino acid substitutions increase the delta G of unfolding between 0.3 and 1.3 kcal/mol at 58.5 degrees C. The combination of these six individual stabilizing mutations together into one subtilisin BPN' molecule was found to result in approximately independent and additive increases in the delta G of unfolding to give a net increase of 3.8 kcal/mol (58.5 degrees C). Thermodynamic stability was also shown to be related to resistance to irreversible inactivation, which included elevated temperatures (65 degrees C) or extreme alkalinity (pH 12.0). Under these denaturing conditions, the rate of inactivation of the combination variant is approximately 300 times slower than that of the wild-type subtilisin BPN'. A comparison of the 1.8-A-resolution crystal structures of mutant and wild-type enzymes revealed only independent and localized structural changes around the site of the amino acid side group substitutions.(ABSTRACT TRUNCATED AT 250 WORDS)
Prostacyclin Analogue Iloprost Influences Endothelial Cell-associated Soluble Adhesion Molecules and Growth Factors in Patients with Systemic Sclerosis: A Time Course Study of Serum Concentrations (2009)

Systemic sclerosis is a connective tissue disorder with unclear aetiology and pathogenesis. However, there is evidence that microvascular changes belong to the early symptoms of the disease. These are associated with increased serum levels of markers of endothelium activation, such as adhesion molecules and growth factors. The stable prostacyclin analogue iloprost is licensed for vascular symptoms (Raynaud's phenomenon) and was recently shown to exert short-term effects on these markers. In this study, serum samples (n = 13) from patients with systemic sclerosis were examined for serum levels of vascular cell adhesion molecule-1 (VCAM-1), intercellular cell adhesion molecule-1, E-selectin, endothelin-1 and vascular endothelial growth factor over 6 months after iloprost infusions in order to detect possible long-term effects. Iloprost significantly reduced initially elevated levels of these markers, partly until the end of the observation period (E-selectin, VCAM-1, endothelin-1). These effects provide serological evidence for the benefits of iloprost infusions that are seen clinically in patients with systemic sclerosis.
Dominant negative mutator mutations in the mutS gene of Escherichia coli (1994)

The MutS protein of Escherichia coli is part of the dam-directed MutHLS mismatch repair pathway which rectifies replication errors and which prevents recombination between related sequences. In order to more fully understand the role of MutS in these processes, dominant negative mutS mutations on a multicopy plasmid were isolated by screening transformed wild-type cells for a mutator phenotype, using a Lac+ papillation assay. Thirty-eight hydroxylamine- and 22 N-methyl-N'-nitro-N-nitrosoguanidine-induced dominant mutations were isolated. Nine of these mutations altered the P-loop motif of the ATP-binding site, resulting in four amino acid substitutions. With one exception, the remaining sequenced mutations all caused substitution of amino acids conserved during evolution. The dominant mutations in the P-loop consensus caused severely reduced repair of heteroduplex DNA in vivo in a mutS mutant host strain. In a wild-type strain, the level of repair was decreased by the dominant mutations to between 12 to 90% of the control value, which is consistent with interference of wild-type MutS function by the mutant proteins. Increasing the wild-type mutS gene dosage resulted in a reversal of the mutator phenotype in about 60% of the mutant strains, indicating that the mutant and wild-type proteins compete. In addition, 20 mutant isolates showed phenotypic reversal by increasing the gene copies of either mutL or mutH. There was a direct correlation between the levels of recombination and mutagenesis in the mutant strains, suggesting that these phenotypes are due to the same function of MutS.
Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds (2012)

We report the stabilization of the human IgG1 Fc fragment by engineered intradomain disulfide bonds. One of these bonds, which connects the N-terminus of the CH3 domain with the F-strand, led to an increase of the melting temperature of this domain by 10°C as compared to the CH3 domain in the context of the wild-type Fc region. Another engineered disulfide bond, which connects the BC loop of the CH3 domain with the D-strand, resulted in an increase of T(m) of 5°C. Combined in one molecule, both intradomain disulfide bonds led to an increase of the T(m) of about 15°C. All of these mutations had no impact on the thermal stability of the CH2 domain. Importantly, the binding of neonatal Fc receptor was also not influenced by the mutations. Overall, the stabilized CH3 domains described in this report provide an excellent basic scaffold for the engineering of Fc fragments for antigen-binding or other desired additional or improved properties. Additionally, we have introduced the intradomain disulfide bonds into an IgG Fc fragment engineered in C-terminal loops of the CH3 domain for binding to Her2/neu, and observed an increase of the T(m) of the CH3 domain for 7.5°C for CysP4, 15.5°C for CysP2 and 19°C for the CysP2 and CysP4 disulfide bonds combined in one molecule.
Teletext/Viewdata LSI (1979)

Teletext and Viewdata are the generic names for two basically similar systems for displaying pages or frames of useful information on the screen of a domestic television set.
Building artificial networks of protein molecular motors (1997)

This study attempts to assess the feasibility of building purposefully designed molecular motor arrays, the proteins responsible for the movements of the living organisms and cells. The 'building' process used high-resolution e-beam patterning, originating in semiconductor technology, upgraded to make biomicrolithography compatible with the patterning of bioactive molecules. The material used as a scaffold for the array [a copolymer of poly(tert-butyl-methacrylate/methyl- methacrylate)] was tailored to exhibit large difference in hydrophobicity when exposed to e-beam exposure. The e-beam patterning exposure-induced difference in hydrophobicity is responsible for the selective attachment of the myosin molecules on the patterned deep-submicron 'tracks,' and the higher concentration of 'guiding' molecules selectively confines the movement of the actin filaments.
Activity recognition: linking low-level sensors to high-level intelligence (2009)

Sensors provide computer systems with a window to the outside world. Activity recognition “sees” what is in the window to predict the locations, trajectories, actions, goals and plans of humans and objects. Building an activity recognition system requires a full range of interaction from statistical inference on lower level sensor data to symbolic AI at higher levels, where prediction results and acquired knowledge are passed up each level to form a knowledge food chain. In this article, I will give an overview of some of the current activity recognition research works and explore a life-cycle of learning and inference that allows the lowestlevel radio-frequency signals to be transformed into symbolic logical representations for AI planning, which in turn controls the robots or guides human users through a sensor network, thus completing a full life cycle of knowledge. 1
Insulin dosage adjustment in diabetes (1992)

This paper introduces a new apparatus and method that suits to measuring the water vapor transmission rate (WVTR) of wound covering materials. WVTR of synthetic materials, amniotic membrane, porcine and cadaveric skin was measured under different conditions of four temperatures, three mediums, and "water cup" or "inverted water cup". The temperature was in close relationship with the WVTR except Omiderm, amniotic membrane, and cadaveric skin between 30 degrees C and 37 degrees C groups. The medium did not affect the WVTR (P > 0.05). There was significant difference between the "water cup" and "inverted water cup" groups(P < 0.01). This study suggests that the apparatus is easy-to-operate, reliable and suitable for the measurement of WVTR of burn wound coverings and other membranous materials.
Reduction in Carotid Arterial Wall Thickness Using Lovastatin and Dietary Therapy (1996)

Lipid-lowering therapy reverses the progression of early, preintrusive atherosclerosis of the carotid artery. Both cholesterol-rich and triglyceride-rich lipoproteins correlate with the progression of early, preintrusive atherosclerosis of the carotid artery. These findings, together with earlier reports of the effects of lovastatin therapy on the progression of atherosclerosis of the coronary arteries, indicate that carotid arterial far wall intima-media thickness is a useful end point for anti-atherosclerosis trials.
Random walks on the click graph (2007)

Search engines can record which documents were clicked for which query, and use these query-document pairs as "soft" relevance judgments. However, compared to the true judgments, click logs give noisy and sparse relevance information. We apply a Markov random walk model to a large click log, producing a probabilistic ranking of documents for a given query. A key advantage of the model is its ability to retrieve relevant documents that have not yet been clicked for that query and rank those effectively. We conduct experiments on click logs from image search, comparing our ("backward") random walk model to a different ("forward") random walk, varying parameters such as walk length and self-transition probability. The most effective combination is a long backward walk with high self-transition probability.
Genetic control of susceptibility to Salmonella typhimurium in mice: role of the LPS gene. (1980)

Abstract C3H/HeJ mice are poorly responsive to all of the biologic effects of bacterial lipopolysaccharides (LPS) and are also, paradoxically, highly susceptible to Salmonella typhimurium infection. The defective response of these mice to LPS is governed by the Lpsd allele, but the gene(s) controlling their response to the bacterium has not been identified. Therefore, genetic analyses were performed to characterize the gene(s) responsible for the susceptibility of C3H/HeJ mice to S. typhimurium and to assess the influence of the Lpsd allele on this sensitivity to infection. The 50% lethal dose (LD50) of S. typhimurium for C3H/HeJ mice was &amp;lt;2, whereas the LD50 for other genetically related but LPS-responsive C3H substrains was ≥2 × 103. The susceptibility gene was distinct from both of the genes previously reported to confer susceptibility to murine typhoid (Ity* and xid). The F1 progeny derived from crosses of C3H/HeJ mice with the highly susceptible C57BL/6J strain (Ity8/Ity8) were resistant (LD50 ≥8 × 103), which indicated that the C3H/ HeJ susceptibility gene was distinct from Ity8. Furthermore, the gene(s) that governed the susceptibility of C3H/HeJ mice to S. typhimurium was also distinct from the X-linked susceptibility gene (xid) because both (C3H/ HeJ × C3H/HeN)F1 males and females were resistant (LD50 ≥ 1 × 104). To determine whether susceptibility to S. typhimurium was due to the expression of Lpsd, a backcross linkage analysis was performed by using the progeny derived from C3H/HeJ and (C3H/HeJ × C57BL/6J)F1 parents. There was a close correlation between a defective LPS proliferative response and susceptibility, since 13 out of 14 of such mice succumbed to infection whereas only 2 out of 13 of LPS-responsive animals died. The susceptibility of backcross mice was also closely linked to another chromosome-4 locus, Mup-1a. These data strongly suggest that the susceptibility of C3H/HeJ mice to S. typhimurium is due either to the Lpsd allele or to a closely linked gene.
Enzyme inhibition by allicin, the active principle of garlic (1956)

Research Article| July 01 1956 Enzyme inhibition by allicin, the active principle of garlic E. D. Wills E. D. Wills 1Department of Biochemistry and Chemistry, Medical College of St Bartholomew's Hospital, London, E.C. 1 Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1956) 63 (3): 514–520. https://doi.org/10.1042/bj0630514 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation E. D. Wills; Enzyme inhibition by allicin, the active principle of garlic. Biochem J 1 July 1956; 63 (3): 514–520. doi: https://doi.org/10.1042/bj0630514 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1956 CAMBRIDGE UNIVERSITY PRESS1956 Article PDF first page preview Close Modal You do not currently have access to this content.
Revisiting the Kinetics and Mechanism of Bromate-Bromide Reaction (2001)

The bromate-bromide reaction was investigated in an acidity range not studied yet. The reaction was followed at the Br2/Br3- isosbestic point (lambda = 446 nm). It was observed a first-order behavior for bromate and bromide ions and a second-order behavior for H+ ion that results in the rate law nu = k[BrO3-][Br- ][H+]². This rate law suggests a mechanism involving two successive protonation of bromate followed by the interaction of the intermediate species H2BrO3+ with bromide. These results disagree with the obtained by other authors who observed a second-order behavior for the bromide and first-order for H+, and have proposed intermediate species like H2Br2O3 and HBr2O3-. The second-order for [H+] observed in the range 0.005 <= [H+] <= 2.77 mol L-1 sets down that the pKa of bromic acid, HBrO3, must be lower than -0.5 (T = 25 °C), different from all other values for this pKa proposed in the literature.
Identification of Distinct Psychosis Biotypes Using Brain-Based Biomarkers (2015)

These data illustrate how multiple pathways may lead to clinically similar psychosis manifestations, and they provide explanations for the marked heterogeneity observed across laboratories on the same biomarker variables when DSM diagnoses are used as the gold standard.
Genetic Immunization of BALB/c mice with a Plasmid Bearing the Gene Coding for a Hybrid Merozoite Surface Protein 1-Hepatitis B Virus Surface Protein Fusion Protects Mice against LethalPlasmodium chabaudi chabaudiPC1 Infection (2000)

The genetic immunization of rodents with a plasmid coding for a Plasmodium chabaudi merozoite surface protein 1 (C terminus)-hepatitis B virus surface fusion protein (pPcMSP1(19)-HBs) provided protection of mice against subsequent lethal challenge with P. chabaudi chabaudi PC1-infected red blood cells. The percentage of survivor mice was higher in DNA-immunized mice than in animals immunized with a recombinant rPcMSP1(19)- glutathione S-transferase fusion protein administered in Freund adjuvant. In all mice immunized with the pPcMSP1(19)-HBs, a Th1-specific response, including the production of anti-MSP1(19)-specific immunoglobulins predominantly of the immunoglobulin G2a subtype and reacting almost exclusively against discontinuous epitopes, was elicited. The coinjection of Th1-type cytokine-expressing plasmids (gamma interferon, interleukin-2, and granulocyte-macrophage colony-stimulating factor) mostly abolished protection and boosting of MSP1(19)-specific antibodies. The inclusion of a lymph node-targeting signal did not significantly increase protection. These data provide further evidence that MSP1(19)-HBs DNA constructs might be useful as components of a genetic vaccine against the asexual blood stages of Plasmodium.
Vision assisted control for manipulation using virtual fixtures (2002)

The "steady hand" concept is a way of providing assistance for direct manipulation by applying constraints on the motion of a tool shared by a user and a robot. We explore in detail one family of constraints: virtual fixtures for use in path following tasks. Vision is used to sense the desired path, and then the robot encourages motion toward and along the path through a direction-based control law. This "soft" virtual fixture allows the user to move in other, non-preferred directions, maintaining the user's sense of autonomy and control. Experimental results show that user performance in assisted path following improves with virtual fixture augmentation, and differs with varying fixture compliance.
[Variations in the P. aeruginosa polysaccharide synthesis conditioned by aminosugars (author's transl)]. (1980)

The production of capsular and extracellular polysaccharides by P. aeruginosa species was conditioned by the presence of hexosamines in the growth media. Glucosamine and most remarkably galactosamine, strongly reduced the production of extracellular polysaccharides. Studies on the dynamics of the polysaccharide production show that these products are used by the cells after the carbon source (glucose of glucosamine) of the growth medium has been exhausted. These results suggest the presence of adaptative hexosaminidases. The concentration of total hexosamines in the polysaccharides changed according to the carbon source (glucose, glucosamine or galactosamine) and so did the molecular composition as demonstrate by IR spectrometry. IR spectra show variations in the intensity of some characteristic bands, as those located at 1,650-1,560 cm-1 (N-acetyl-hexosamines), 1,420 and 1,320 cm-1 (aminosugars) and at 1,380 cm-1. This last band disappears from polysaccharides after prolonged incubation. Bands between 1,300 and 700 cm-1 varied and even disappeared according to the aminosugar employed and the length of the incubation period.
Teaching Real Property Law as Real Estate Lawyering (1996)

This Article describes an alternative way of teaching the Property course so as to have students appreciate that the rules covered there are relevant to the everyday practice of law. It concentrates on the use lawyers make of those rules, rather than treating the rules as ends in themselves.