gebhart/patrepeval huggingface dataset:
Fibronectin, Laminin, Type I, III and IV Collagens in Duchenne's Muscular Dystrophy, Congenital Muscular Dystrophies and Congenital Myopathies: An Immunocytochemical Study (1985)

The distribution pattern of fibronectin, laminin, type I, III and IV collagens in human skeletal muscle was studied by immunofluorescence. In normal muscle, as well as in congenital myopathies (CM), type I and III collagens were localized in the endomysium and the perimysium. Laminin and type IV collagen delineated precisely each muscle fiber but did not stain the perimysium. In Duchenne's muscular dystrophy (DMD) as well as in congenital muscular dystrophies (CMD) the extensive proliferation of connective tissue consisted mainly of fibronectin and type I and III collagens. Laminin and type IV collagen delineated principally the basal lamina but suprisingly were found to be distributed to some extent all over the extracellular matrix. No disease-specific accumulation of components of the extracellular matrix was found which would enable us to differentiate these last two diseases, though the immunofluorescence reactions for all components were stronger in DMD than in CMD.
Giant magnetoresistance in magnetic multilayered nanowires (1994)

Giant magnetoresistance (GMR) is observed in a new type of nanostructured material consisting of magnetic multilayered nanowires formed by electrodeposition into nanometer-sized pores of a template polymer membrane. The composition of these nanowires is modulated over nanometer length scales with distinct magnetic and nonmagnetic metallic layers. Magnetoresistance measurements with the current perpendicular to the layers were performed on the array of parallel nanowires. GMR of about 15% was observed at room temperature on Co/Cu multilayered nanowires.
Blind Multiuser Receiver in Rayleigh Fading Channel (2006)

We propose and investigate a code-division multiple-access (CDMA) communication system using independent component analysis (ICA) for blind signal detection purposes. The proposed blind ICA based CDMA systems employ short spreading sequences in the presence of Rayleigh fading channels. This approach is capable of countering the presence of multiple access interference (MAI) in CDMA fading channels. The aim of the blind system is to include the ICA algorithm within a wireless receiver in order to reduce the level of multiuser interference in communication systems. Unlike the conventional MMSE receiver, the proposed ICA blind multiuser detector is made robust with no-training sequences and perfect knowledge of signature waveforms. It has achieved nearly the same performance of the conventional training based MMSE receiver. Several comparisons and experiments are performed based on examining bit error rate (BER) performance in AWGN and Rayleigh fading channels in order to verify the validity of the proposed blind ICA multiuser detector
A Role for Polyploidy in the Tumorigenicity of Pim-1-Expressing Human Prostate and Mammary Epithelial Cells (2008)

Our results support the notion that polyploidy can promote chromosomal instability and the initiation of tumorigenesis in human epithelial cells.
Occurrence of S‐adenosylhomocysteine hydrolase in prokaryote cells (1984)

The occurrence of S-adenosylhomocysteine hydrolase (EC 3.3.1.1) was found in a variety of prokaryotes. These prokaryotes did not exhibit any activities of S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) and S-ribosyl-homocysteine hydrolase (EC 3.3.1.3), which had been the generally accepted prokaryote enzymes for the regeneration of free homocysteine from S-adenosylhomocysteine in the activated methyl cycle. In these prokaryotes S-adenosylhomocysteine hydrolase was suggested to be the only enzyme functioning for the regeneration of free homocysteine by enzymological and immunochemical studies. S-Adenosylhomocysteine hydrolase was purified and crystallized from cells of a prokaryote, Alcaligenes faecalis. The purified enzyme was found to be homogeneous on ultracentrifugation and gel electrophoresis. Its relative molecular mass is approximately 280 000 and it is composed of six identical subunits with a Mr of approximately 48 000. The NH2-terminal and COOH-terminal amino acids are lysine and tyrosine respectively. The enzyme contains 6 mol NAD/mol. Some nucleosides, such as formycin A, nebularine, adenosine N1-oxide and so on, are able to substitute for adenosine yielding the corresponding S-nucleosidylhomocysteine congeners. Modification of the 5'-hydroxymethyl group in adenosine leads to the most potent inhibition of the thioether formation of homocysteine with adenosine. The enzyme from A. faecalis has some immunological similarities to other prokaryote S-adenosylhomocysteine hydrolases, but is different from the enzymes of animal sources.
The art of PS2 : the complete set of figures, panels and tables from introduction to protein structure (1999)

A contemporary account of the principles of protein structure, with examples of proteins in their biological context. Includes chapters on the general principles, specific examples of structures and on determining and predicting.
One-pot synthesis of optically active cyanohydrin acetates from aldehydes via lipase-catalyzed kinetic resolution coupled with in situ formation and racemization of cyanohydrins (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOne-pot synthesis of optically active cyanohydrin acetates from aldehydes via lipase-catalyzed kinetic resolution coupled with in situ formation and racemization of cyanohydrinsMinoru Inagaki, Jun Hiratake, Takaaki Nishioka, and Junichi OdaCite this: J. Org. Chem. 1992, 57, 21, 5643–5649Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/jo00047a016https://doi.org/10.1021/jo00047a016research-articleACS PublicationsRequest reuse permissionsArticle Views709Altmetric-Citations135LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Vector field visualization (1994)

We present two new techniques for rendering 3D vector fields in scientific visualisation. We also present a new technique called texture splats for rendering these volumes efficiently.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Expanded Tropism of Primary Human Immunodeficiency Virus Type 1 R5 Strains to CD4 + T-Cell Lines Determined by the Capacity To Exploit Low Concentrations of CCR5 (1999)

Human immunodeficiency virus type 1 (HIV-1) non-syncytium-inducing (NSI) strains predominantly use the chemokine receptor CCR5, while syncytium-inducing (SI) strains use CXCR4. In vitro, SI isolates infect and replicate in a range of CD4(+) CXCR4(+) T-cell lines, whereas NSI isolates usually do not. Here we describe three NSI strains that are able to infect two CD4(+) T-cell lines, Molt4 and SupT1. For one strain, a variant of JRCSF selected in vitro, replication on Molt4 was previously shown to be conferred by a single amino-acid change in the V1 loop (M.T. Boyd et al., J. Virol. 67:3649-3652, 1993). On CD4(+) cell lines expressing different coreceptors, these strains use CCR5 predominantly and do not replicate in CCR5-negative peripheral blood mononuclear cells derived from individuals homozygous for Delta32 CCR5. Furthermore, infection of Molt4 and SupT1 by each of these three strains is potently inhibited by ligands for CCR5, including 2D7, a monoclonal antibody specific for CCR5. CCR5 mRNA was present in both Molt4 and SupT1 by reverse transcription-PCR, although CCR5 protein could not be detected either on the cell surface or in intracellular vesicles. The expanded tropism of the three strains shown here is therefore not due to adaptation to a new coreceptor but due to the capacity to exploit extremely low levels of CCR5 on Molt4 and SupT1 cells. This novel tropism observed for a subset of primary HIV-1 isolates may represent an extended tropism to new CD4(+) cell types in vivo.
Cryogenic trapping for determination of odor concentration (2004)

The sensory testing method applied under Japanese law to measure odor concentration has a lower detection limit of 10 in the specified Odor Index. To measure odor below the limit, a condensing procedure using solid sorbents (Tenax-TA, Unicarbon B and Carbosieve SIII) has been developed and used in Japan. This procedure however cannot condense all odorous substances, and is specifically unsuited to hydrogen sulfide, methyl mercaptan, dimethyl sulfide, ammonia, and other typical odorous substances. In the present study, cryogenic trapping was tested to improve recovery rate. As water in sample air causes choking of the trap tube, vacant pre-columns to condense the water were connected to the Tenax-TA-packed column. The columns were chilled with liquid oxygen before passage of 100 L of sample air. The columns were then heated to 200 degrees C under passage of 50 mL/min of nitrogen carrier gas to desorb odors. The desorbed gases were captured in sampling bags made of polyethylene terephthalate film. The total volume of desorbed gases was approximately 1 L. The method showed good recovery rates for hydrogen sulfide, methyl mercaptan, dimethyl sulfide and ammonia, and was useful for determining low-level odor concentrations during measurement of odor in ambient air at various sites in Osaka City.
Unexpected selectivity in the alkylation of polyazamacrocycles (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnexpected selectivity in the alkylation of polyazamacrocyclesWilliam J. Kruper Jr., Philip R. Rudolf, and Charles A. LanghoffCite this: J. Org. Chem. 1993, 58, 15, 3869–3876Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/jo00067a018https://doi.org/10.1021/jo00067a018research-articleACS PublicationsRequest reuse permissionsArticle Views971Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Deletion of Immunoproteasome Subunits Imprints on the Transcriptome and Has a Broad Impact on Peptides Presented by Major Histocompatibility Complex I molecules (2010)

Proteasome-mediated proteolysis plays a crucial role in many basic cellular processes. In addition to constitutive proteasomes (CPs), which are found in all eukaryotes, jawed vertebrates also express immunoproteasomes (IPs). Evidence suggests that the key role of IPs may hinge on their impact on the repertoire of peptides associated to major histocompatibility complex (MHC) I molecules. Using a label-free quantitative proteomics approach, we identified 417 peptides presented by MHC I molecules on primary mouse dendritic cells (DCs). By comparing MHC I-associated peptides (MIPs) eluted from primary DCs and thymocytes, we found that the MIP repertoire concealed a cell type-specific signature correlating with cell function. Notably, mass spectrometry analyses of DCs expressing or not IP subunits MECL1 and LMP7 showed that IPs substantially increase the abundance and diversity of MIPs. Bioinformatic analyses provided evidence that proteasomes harboring LMP7 and MECL1 have specific cleavage preferences and recognize unstructured protein regions. Moreover, while differences in MIP repertoire cannot be attributed to potential effects of IPs on gene transcription, IP subunits deficiency altered mRNA levels of a set of genes controlling DC function. Regulated genes segregated in clusters that were enriched in chromosomes 4 and 8. Our peptidomic studies performed on untransfected primary cells provide a detailed account of the MHC I-associated immune self. This work uncovers the dramatic impact of IP subunits MECL1 and LMP7 on the MIP repertoire and their non-redundant influence on expression of immune-related genes.
Preconditioned time domain electromagnetic migration (1998)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 1998Preconditioned time domain electromagnetic migrationAuthors: Michael S. ZhdanovWeidong LiMichael S. ZhdanovUniversity of Utah, Nobuhiko Shiga Mindeco, Japan and Weidong LiUniversity of Utah, Nobuhiko Shiga Mindeco, Japanhttps://doi.org/10.1190/1.1820463 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1820463FiguresReferencesRelatedDetailsCited ByFinite‐difference EM modeling and imaging in a medium with a vertical axis of symmetryEfthimios Tartaras and Michael Zhdanov4 January 2005 SEG Technical Program Expanded Abstracts 1998ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 1998 Pages: 2092 publication data© 1998 Copyright © 1998 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished: 06 Jan 2005 CITATION INFORMATION Michael S. Zhdanov and Weidong Li, (1998), "Preconditioned time domain electromagnetic migration," SEG Technical Program Expanded Abstracts : 461-464. https://doi.org/10.1190/1.1820463 Plain-Language Summary PDF DownloadLoading ...
Manual versus mechanical cardiopulmonary resuscitation. An experimental study in pigs (2010)

LUCAS-CPR gave significantly higher coronary perfusion pressure and significantly fewer rib fractures than manual CPR in this porcine model.
An Open Trial of Methyltetrahydrofolate in Elderly Depressed Patients (1993)

5-methyltetrahydrofolate (MTHF) is a naturally occurring substance involved in the synthesis of s-adenosyl-l-methionine (SAMe), a major source of methyl groups in the brain. To assess the efficacy of a gastro-resistant, oral preparation of MTHF, 20 elderly patients with a DSM-III-R diagnosis of depressive disorder and a HAM-D-21 score > or = 18 underwent 6-weeks of open-label treatment with 50 mg per day of oral MTHF. Of these 20 patients, 16 completed at least 4 weeks of treatment and showed a markedly significant improvement in their depressive symptoms at endpoint, with 81% of them being considered responders. There were no clinically relevant changes in the routine laboratory tests during the study, and no adverse events considered to be definitely drug-related were reported.
Serum Immune-Related Proteins are Differentially Expressed during Hibernation in the American Black Bear (2013)

Hibernation is an adaptation to conserve energy in the face of extreme environmental conditions and low food availability that has risen in several animal phyla. This phenomenon is characterized by reduced metabolic rate (∼25% of the active basal metabolic rate in hibernating bears) and energy demand, while other physiological adjustments are far from clear. The profiling of the serum proteome of the American black bear (Ursus americanus) may reveal specific proteins that are differentially modulated by hibernation, and provide insight into the remarkable physiological adaptations that characterize ursid hibernation. In this study, we used differential gel electrophoresis (DIGE) analysis, liquid chromatography coupled to tandem mass spectrometry, and subsequent MASCOT analysis of the mass spectra to identify candidate proteins that are differentially expressed during hibernation in captive black bears. Seventy serum proteins were identified as changing by ±1.5 fold or more, out of which 34 proteins increased expression during hibernation. The majority of identified proteins are involved in immune system processes. These included α2-macroglobulin, complement components C1s and C4, immunoglobulin μ and J chains, clusterin, haptoglobin, C4b binding protein, kininogen 1, α2-HS-glycoprotein, and apoplipoproteins A-I and A-IV. Differential expression of a subset of these proteins identified by proteomic analysis was also confirmed by immunodetection. We propose that the observed serum protein changes contribute to the maintenance of the hibernation phenotype and health, including increased capacities for bone maintenance and wound healing during hibernation in bears.
An information system for corporate users: wide area information servers (1991)

For both residents and interdisciplinary staff, application of a cognitive model that clearly defines goals and expectations for ACP discussions prior to meeting with patients and families improves rates of ACP discussions.
A 900MHz high efficiency and linearity adaptive CMOS power amplifier (2011)

A high efficiency, linear, adaptive radio frequency (RF) power amplifier (PA) is presented. The working frequency is about 900MHz. The circuit employs a self-bias cascode for a higher performance and an adaptive bias to enhance linear and efficiency of the PA as well. This power amplifier is designed with the SMIC 0.18um CMOS RF process. The power supply is 1.8V. The simulation results show that the PA has a power gain of 30dB. In addition, the Power-Added Efficiency is over 33%.
Slug-Dependent Upregulation of L1CAM Is Responsible for the Increased Invasion Potential of Pancreatic Cancer Cells following Long-Term 5-FU Treatment (2015)

Our findings establish Slug-induced L1CAM expression as a mediator of a chemoresistant and migratory phenotype in pancreatic adenocarcinoma cells.
DNA vaccination primes MHC class I-restricted, simian virus 40 large tumor antigen-specific CTL in H-2d mice that reject syngeneic tumors (1996)

We investigated the stimulation of a MHC class I-restricted response of CTL to the SV40 large tumor Ag (T-Ag) by different vaccination strategies in H-2d and H-2b mice. Immunization with plasmid DNA or exogenous T-Ag, or infection with SV40, primed CTL to T-Ag in H-2b mice; these three different types of Ag delivery primed T-Ag-specific CTL populations with similar epitope/restriction specificities. In H-2d mice, i.m. immunization with plasmid DNA, but neither immunization with exogenous protein Ag nor SV40 infection, primed CTL to T-Ag. T-Ag-specific H-2d CTL were primed by DNA-based immunization in vivo, expressed the CD4-CD8+ phenotype, and were L(d)-restricted. In H-2d (DBA/2) mice, T-Ag-specific immune responses primed by plasmid DNA injection, but not those primed by exogenous T-Ag or SV40 infection, mediated CD8+ CTL-dependent rejection of T-Ag-expressing P815/T tumor grafts. The data indicate that immunization by plasmid DNA injection is an efficient strategy to induce class I-restricted CTL responses against oncogene-encoded Ags of low immunogenicity that mediate tumor rejection.
Exploring commonalities across participants in the neural representation of objects (2011)

The question of whether the neural encodings of objects are similar across different people is one of the key questions in cognitive neuroscience. This article examines the commonalities in the internal representation of objects, as measured with fMRI, across individuals in two complementary ways. First, we examine the commonalities in the internal representation of objects across people at the level of interobject distances, derived from whole brain fMRI data, and second, at the level of spatially localized anatomical brain regions that contain sufficient information for identification of object categories, without making the assumption that their voxel patterns are spatially matched in a common space. We examine the commonalities in internal representation of objects on 3T fMRI data collected while participants viewed line drawings depicting various tools and dwellings. This exploratory study revealed the extent to which the representation of individual concepts, and their mutual similarity, is shared across participants.
Neural differentiation of patient specific iPS cells as a novel approach to study the pathophysiology of multiple sclerosis (2011)

The recent introduction of technologies capable of reprogramming human somatic cells into induced pluripotent stem (iPS) cells offers a unique opportunity to study many aspects of neurodegenerative diseases in vitro that could ultimately lead to novel drug development and testing. Here, we report for the first time that human dermal fibroblasts from a patient with relapsing-remitting Multiple Sclerosis (MS) were reprogrammed to pluripotency by retroviral transduction using defined factors (OCT4, SOX2, KLF4, and c-MYC). The MSiPS cell lines resembled human embryonic stem (hES) cell-like colonies in morphology and gene expression and exhibited silencing of the retroviral transgenes after four passages. MSiPS cells formed embryoid bodies that expressed markers of all three germ layers by immunostaining and Reverse Transcriptase (RT)-PCR. The injection of undifferentiated iPS cell colonies into immunodeficient mice formed teratomas, thereby demonstrating pluripotency. The MSiPS cells were successfully differentiated into mature astrocytes, oligodendrocytes and neurons with normal karyotypes. Although MSiPS-derived neurons displayed some differences in their electrophysiological characteristics as compared to the control cell line, they exhibit properties of functional neurons, with robust resting membrane potentials, large fast tetrodotoxin-sensitive action potentials and voltage-gated sodium currents. This study provides for the first time proof of concept that disease cell lines derived from skin cells obtained from an MS patient can be generated and successfully differentiated into mature neural lineages. This represents an important step in a novel approach for the study of MS pathophysiology and potential drug discovery.