gebhart/patrepeval huggingface dataset:
Repairing the brain: Trophic factor or transplant? (1995)

Abstract Three experiments on neural grafting with adult rat hosts are described. Working memory impairments were produced by lesioning the hippocampus or severing its connections with the septum by ablating the fimbria-fornix. The results suggest that the survival and growth of a neural graft, whether an autograft or a xenograft, is not a necessary condition for functional recovery on a task tapping working memory.
Ablation of Rat TRPV1-Expressing Adelta/C-Fibers with Resiniferatoxin: Analysis of Withdrawal Behaviors, Recovery of Function and Molecular Correlates (2010)

Behavioral recovery following peripheral RTX treatment is linked to regeneration of TRPV1-expressing Adelta and C-fibers and sustained expression of molecular markers. Infrared laser stimulation is a potentially valuable tool for evaluating the behavioral role of Adelta fibers in pain and pain control.
The Discovery of Tadalafil:  A Novel and Highly Selective PDE5 Inhibitor. 2: 2,3,6,7,12,12a-hexahydropyrazino[1‘,2‘:1,6]pyrido[3,4-b]indole-1,4-dione Analogues (2003)

Modification of the hydantoin ring in the previously described lead compound 2a has led to the discovery of compound 12a, tadalafil, a highly potent and highly selective PDE5 inhibitor. The replacement of the hydantoin in compound 2a by a piperazinedione ring led to compound cis-11a which showed similar PDE5 inhibitory potency. Introduction of a 3,4-methylenedioxy substitution on the phenyl ring in position 6 led to a potent PDE5 inhibitor cis-11c with increased cellular potency. Optimization of the chain on the piperazinedione ring led to the identification of the racemic cis-N-methyl derivative 11i. High diastereospecificity for PDE5 inhibition was observed in the piperazinedione series with the cis-(6R,12aR) enantiomer displaying the highest PDE5 inhibitory activity. The piperazinedione 12a, tadalafil (GF196960), has been identified as a highly potent PDE5 inhibitor (IC(50) = 5 nM) with high selectivity for PDE5 vs PDE1-4 and PDE6. Compound 12a displays 85-fold greater selectivity vs PDE6 than sildenafil 1. 12a showed profound and long-lasting blood pressure lowering activity (30 mmHg/>7 h) in the spontaneously hypertensive rat model after oral administration (5 mg/kg).
Delay Time of Hemoglobin S Polymerization Prevents Most Cells from Sickling in Vivo (1987)

A laser photolysis technique has been developed to assess the quantitative significance of the delay time of hemoglobin S gelation to the pathophysiology of sickle cell disease. Changes in the saturation of hemoglobin S with carbon monoxide produced by varying the intensity of a photolytic laser beam were used to simulate changes in the saturation of oxyhemoglobin S produced by variations in oxygen pressure. The presence of polymer at steady-state saturation with carbon monoxide was determined by measurement of the kinetics of gelation after complete photodissociation. The kinetics are a very sensitive probe for polymer since small amounts of polymerized hemoglobin increase the rate of nucleation sufficiently to eliminate the delay period. First, the equilibrium gelation properties of partially photodissociated carbonmonoxyhemoglobin S were shown to be the same as partially oxygenated hemoglobin S, and the method was then used to determine the effect of saturation on the formation and disappearance of polymers in individual sickle cells. The saturation at which polymers first formed upon deoxygenation was much lower than the saturation at which polymers disappeared upon reoxygenation. The results indicate that at venous saturations with oxygen, gelation takes place in most cells at equilibrium, but is prevented from occurring in vivo because the delay times are sufficiently long that most cells return to the lungs and are reoxygenated before polymerization has begun.
Immunization with PCEP microparticles containing pertussis toxoid, CpG ODN and a synthetic innate defense regulator peptide induces protective immunity against pertussis (2011)

We investigated the efficacy of a novel microparticle (MP) based vaccine formulation consisting of pertussis toxoid (PTd), polyphosphazene (PCEP), CpG ODN 10101 and synthetic cationic innate defense regulator peptide 1002 (IDR) against Bordetella pertussis in mice. We studied whether encapsulation of these IDR-CpG ODN complexes into polyphosphazene-based microparticles further enhanced their immunomodulatory activity compared to soluble formulations containing PCEP (SOL), or without PCEP (AQ). In vitro stimulation of murine macrophages showed MP induced significantly higher levels of pro-inflammatory cytokines. When assessed in a B. pertussis infection challenge model, a single immunization with MP formulation led to significantly lower bacterial loads compared to other formulations and non-vaccinated animals. ELISPOT of splenocytes showed that MP group mice had significantly higher number of antigen-specific IL-17 secreting cells. The cytokine profile in lung homogenates of MP group mice after challenge showed significantly higher amounts of MCP-1, TNF-α, IFN-γ, IL-12 and IL-17 and significantly lowered IL-10 levels suggesting a strong Th1 shift. Protection was observed against challenge infection with B. pertussis. On the other hand protective immune responses elicited in Quadracel(®) immunized mice were Th2 skewed. Hence, we conclude that formulation of PTd, PCEP, CpG ODN and IDR into MP generates a protective immune response in mice against pertussis emphasizing the potential of MP as a delivery vehicle for the potential development of single-shot vaccines.
Gonococcal pili. Primary structure and receptor binding domain. (1984)

The complete amino acid sequence of pilin from gonococcal strain MS11 and the sequence of constant and variable regions from strain R10 pilin have been determined in order to elucidate the structural basis for adherence function, antigenic diversity, and polymeric structure. The MS11 pilin sequence consists of 159 amino acids in a single polypeptide chain with two cysteines in disulfide linkage and serine-bonded phosphate residues. TC-2 (31-111), a soluble monomeric pilus peptide prepared by arginine-specific digestion, bound human endocervical, but not buccal or HeLa cells and therefore is postulated to encompass the receptor binding domain. Variable regions of CNBr-3 appear to confer antigenic diversity and comprise segments in which changes in the position of charged residues occur in hydrophilic, beta-turns. Residues 2-21 and 202-221 of gonococcal pilins and lower eucaryotic actins, respectively, exhibit 50% homology. When these residues are arranged at intervals of 100 degrees of arc on "helical wheels," the identical amino acids comprise a hydrophobic face on one side of the helix. This observation, the hydrophobic character of this region and the tendency for TC-1 (residues 1-30) to aggregate in water, suggest that this stretch interacts with other subunits to stabilize polymeric structure.
A survey of positioning techniques and location based services in wireless networks (2015)

Positioning techniques are known in a wide variety of wireless radio access technologies. Traditionally, Global Positioning System (GPS) is the most popular outdoor positioning system. Localization also exists in mobile networks such as Global System for Mobile communications (GSM). Recently, Wireless Local Area Networks (WLAN) become widely deployed, and they are also used for localizing wireless-enabled clients. Many techniques are used to estimate client position in a wireless network. They are based on the characteristics of the received wireless signals: power, time or angle of arrival. In addition, hybrid positioning techniques make use of the collaboration between different wireless radio access technologies existing in the same geographical area. Client positioning allows the introduction of numerous services like real-time tracking, security alerts, informational services and entertainment applications. Such services are known as Location Based Services (LBS), and they are useful in both commerce and security sectors. In this paper, we explain the principles behind positioning techniques used in satellite networks, mobile networks and Wireless Local Area Networks. We also describe hybrid localization methods that exploit the coexistence of several radio access technologies in the same region, and we classify the location based services into several categories. When localization accuracy is improved, position-dependant services become more robust and efficient, and user satisfaction increases.
Functional Co-localization of Transfected Ca2+-stimulable Adenylyl Cyclases with Capacitative Ca2+ Entry Sites (1996)

Three adenylyl cyclases (ACI, ACIII, and ACVIII) have been described, which are putatively Ca(2+)-stimulable, based on in vitro assays. However, it is not clear that these enzymes can be regulated by physiological rises in [Ca2+]i when expressed in intact cells. Furthermore, it is not known whether transfected adenylyl cyclases might display the strict requirement for capacitative Ca2+ entry that is shown by the Ca(2+)-inhibitable ACVI, which is indigenous to C6-2B glioma cells (Chiono, M., Mahey, R., Tate, G., and Cooper, D. M. F. (1995) J. Biol. Chem. 270, 1149-1155). In the present study, ACI, ACIII, and ACVIII were heterologously expressed in HEK 293 cells, and conditions were devised that distinguished capacitative Ca2+ entry from both internal release and nonspecific elevation in [Ca2+]i around the plasma membrane. Remarkably, not only were ACI and ACVIII largely insensitive to Ca2+ release from stores, but they were robustly stimulated only by capacitative Ca2+ entry and not al all by a substantial increase in [Ca2+]i at the plasma membrane elicited by ionophore. (ACIII, reflecting its feeble in vitro sensitivity to Ca2+, was unaffected by any [Ca2+]i rise.) These results suggest a quite unsuspected, essential association of Ca(2+)-sensitive adenylyl cyclases with capacitative Ca2+ entry sites, even when expressed heterologously.
Electrodynamics at rough metal surfaces: Photochemistry and luminescence of adsorbates near metal-island films (1984)

Detailed measurements of the photochemical and photophysical properties of an adsorbate on discontinuous metal-island films are used to explore the unusual electrodynamics near rough metal surfaces. Several aspects of the properties have been measured: the magnitude the temporal decay of the fluorescence, the shape and temporal evolution of the fluorescence spectrum, and the effects on the spectrum of a photochemical hole-burning process. Dramatic increases in the fluorescent decay rate and decreases in the photochemical reaction rate as well as systematic spectral shifts of the emission of molecules experiencing the different electrodynamic environments on the island film are observed. These results reveal the strong effects of the coupling between the adsorbate and the plasma resonances localized on the islands of the film. We model our results using the electrodynamic picture which has successfully described many aspects of surface-enhanced Raman scattering and other optical processes on island films. The excellent agreement between this model and our results suggests that an important feature of the electrodynamics at these rough metal surfaces is the dipolar character of the couplings between the surface, the adsorbate, and the optical fields.
Separating reflections and lighting using independent components analysis (2003)

The image of an object can vary dramatically depending on lighting, specularities/reflections and shadows. It is often advantageous to separate these incidental variations from the intrinsic aspects of an image. This paper describes how the statistical tool of independent components analysis can be used to separate some of these incidental components. We describe the details of this method and show its efficacy with examples of separating reflections off glass, and separating the relative contributions of individual light sources.
Change in pH of plaque and 10% carbamide peroxide solution during nightguard vital bleaching treatment. (1994)

The purpose of this study was to evaluate in vivo changes in the pH of plaque and of a 10% carbamide peroxide solution occurring within the bleaching guard during a 2-hour nightguard vital bleaching procedure. Baseline pH values for plaque and the carbamide peroxide solution were established. A small hole was placed in the anterior interproximal region of the guard to allow placement of the pH electrode. The pH of the carbamide peroxide solution was measured at 5-minute intervals. After 2 hours, the guard was removed and pH of the plaque was remeasured. The procedure was repeated three times on each of four subjects. The mean baseline pH reading for plaque was 6.31 and mean final pH reading was 6.86. The difference was statistically significant. At initial placement of the carbamide peroxide-filled guard, the mean intraguard pH was 4.50 (range of 2.80 to 7.80). The mean peak intraguard pH of 8.06 (range of 7.30 to 8.43), which was significantly different from baseline, was obtained within 31 minutes. The pH of plaque, saliva, and a 10% carbamide peroxide bleaching solution within the guard increased significantly during bleaching and remained significantly elevated for the duration of the study (2 hours).
Protection against a Lethal Avian Influenza A Virus in a Mammalian System (1999)

ABSTRACT The question of how best to protect the human population against a potential influenza pandemic has been raised by the recent outbreak caused by an avian H5N1 virus in Hong Kong. The likely strategy would be to vaccinate with a less virulent, laboratory-adapted H5N1 strain isolated previously from birds. Little attention has been given, however, to dissecting the consequences of sequential exposure to serologically related influenza A viruses using contemporary immunology techniques. Such experiments with the H5N1 viruses are limited by the potential risk to humans. An extremely virulent H3N8 avian influenza A virus has been used to infect both immunoglobulin-expressing (Ig +/+ ) and Ig −/− mice primed previously with a laboratory-adapted H3N2 virus. The cross-reactive antibody response was very protective, while the recall of CD8 + T-cell memory in the Ig −/− mice provided some small measure of resistance to a low-dose H3N8 challenge. The H3N8 virus also replicated in the respiratory tracts of the H3N2-primed Ig +/+ mice, generating secondary CD8 + and CD4 + T-cell responses that may contribute to recovery. The results indicate that the various components of immune memory operate together to provide optimal protection, and they support the idea that related viruses of nonhuman origin can be used as vaccines.
RNA-Mediated Resistance with Nonstructural Genes from the Tobacco Etch Virus Genome (1995)

Sense RNA-mediated virus resistance has been described for transgenic plants expressing potyviral capsid protein sequences. This study was undertaken to determine if expression of other viral sequences could induce this type of virus resistance. Plants showing highly resistant or 'recovery' phenotypes were generated by expressing the tobacco etch virus (TEV) 6 kDa/21 kDa reading frames. Expression of translatable or untranslatable versions of this TEV sequence produced resistant lines. Highly resistant and recovery phenotype plants expressing TEV coat protein sequences. High transcription rates with low steady-state levels of the transgene transcript generally correlated with resistance. During recovery and induction of the resistance, RNA and protein steady-state levels decreased 5- to 20-fold, while transcription of the transgene continued at a similar level. A posttranscriptional, cellular system eliminating sequences contained in the transgene transcript and viral genome would be consistent with the results.
Overexpression of transcripts originating from the MMSET locus characterizes all t(4;14)(p16;q32)-positive multiple myeloma patients (2005)

Multiple myeloma (MM) is a B-lineage malignancy characterized by diverse genetic subtypes and clinical outcomes. The recurrent immunoglobulin heavy chain (IgH) switch translocation, t(4;14)(p16;q32), is associated with poor outcome, though the mechanism is unclear. Quantitative reverse-transcription-polymerase chain reaction (RT-PCR) for proposed target genes on a panel of myeloma cell lines and purified plasma cells showed that only transcripts originating from the WHSC1/MMSET/NSD2 gene are uniformly dysregulated in all t(4;14)POS patients. The different transcripts detected, multiple myeloma SET domain containing protein (MMSET I), MMSET II, Exon 4a/MMSET III, and response element II binding protein (RE-IIBP), are produced by alternative splicing and alternative transcription initiation events. Translation of the various transcripts, including those from major breakpoint region 4-2 (MB4-2) and MB4-3 breakpoint variants, was confirmed by transient transfection and immunoblotting. Green fluorescent protein (GFP)-tagged MMSET I and II, corresponding to proteins expressed in MB4-1 patients, localized to the nucleus but not nucleoli, whereas the MB4-2 and MB4-3 proteins concentrate in nucleoli. Cloning and localization of the Exon 4a/MMSET III splice variant, which contains the protein segment lost in the MB4-2 variant, identified a novel protein domain that prevents nucleolar localization. Kinetic studies using photobleaching suggest that the breakpoint variants are functionally distinct from wild-type proteins. In contrast, RE-IIBP is universally dysregulated and also potentially functional in all t(4;14)POS patients irrespective of fibroblast growth factor receptor 3 (FGFR3) expression or breakpoint type.
Repair of a recurrent urethrovaginal fistula with an island bulbocavernous musculocutaneous flap. (1993)

This paper describes a case of recurrent postpartum urethrovaginal fistula. The extent of the vaginal tissue loss and the perilesional scarring made direct closure of the defect not practicable. After suturing of the urethra, the anterior vaginal wall was reconstructed with an island bulbocavernous musculocutaneous flap raised from the left labium majus. Seven months after surgery, the flap healed well, and cystography showed a regular voiding without periurethral suffusions. Healing of thedonor site also was aesthetically satisfactory.
Interaction of GTP-binding regulatory proteins with chemosensory receptors. (1987)

GTP-binding regulatory proteins (G-proteins) were identified in chemosensory membranes from the channel catfish, Ictalurus punctatus. The common G-protein beta-subunit was identified by immunoblotting in both isolated olfactory cilia and purified taste plasma membranes. A cholera toxin substrate (Mr 45,000), corresponding to the G-protein that stimulates adenylate cyclase, was identified in both membranes. Both membranes also contained a single pertussis toxin substrate. In taste membranes, this component co-migrated with the alpha-subunit of the G-protein that inhibits adenylate cyclase. In olfactory cilia, the Mr 40,000 pertussis toxin substrate cross-reacted with antiserum to the common amino acid sequence of G-protein alpha-subunits, but did not cross-react with antiserum to the alpha-subunit of the G-protein from brain of unknown function. The interaction of G-proteins with chemosensory receptors was determined by monitoring receptor binding affinity in the presence of exogenous guanine nucleotides. L-Alanine and L-arginine bind with similar affinity to separate receptors in both olfactory and gustatory membranes from the catfish. GTP and a nonhydrolyzable analogue decreased the affinity of olfactory L-alanine and L-arginine receptors by about 1 order of magnitude. In contrast, the binding affinities of the corresponding taste receptors were unaffected. These results suggest that olfactory receptors are functionally coupled to G-proteins in a manner similar to some hormone and neurotransmitter receptors.
Effects of Intravenous Infusion of Hypotonic Hartman's Solution, with Volumes of Essential Water Loses, on Plasma Volume and Acid-base Equilibrium in Nondehydrated Heifers (2001)

The effects of intravenous infusion of hypotonic Hartman's replacement fluids (HG) on plasma volume and acid-base equilibrium were examined. Tests were made twice on 4 heifers. The animals were infused with either 30ml/kg or 60ml/kg of HG at a flow rate of 20ml/kg/hr and were allowed at least 20 days to recover between infusions. After fluid replacement, they were monitored for 270 minutes. At the end of fluid replacement, relative plasma volume (rPV) increased to 122.3±4.1%(t=90) in the animals receiving 30 ml/kg and 129.5±4.7%(t=180) in animals receiving 60ml/kg. No significant difference in rPV was observed between the 2 groups. No significant changes were observed in acid-base equilibrium and serum osmotic pressure in heifers receiving intravenous HG infusions of 30 ml/kg or 60ml/kg. These findings indicate that hypotonicsolution infusions of 30ml/kg or 60ml/kg at a flow rate of 20ml/kg/hr can be administered to cattle.effectively and safely.
L-Tyrosine-3, 5- 3 H Assay for Tyrosinase Development in Skin of Newborn Hamsters (1969)

Tyrosinase in hamster skin, measured by the production of (3)HOH from L-tyrosine-3,5-(3)H, increases about sevenfold in specific activity from the 14-day fetus to day 6 of life. Albino hamsters exhibit no activity, but enzyme is present in the ventral (white) areas of the Syrian hamster. 3,4-Dihydroxy-L-phenylalanine is required as a cosubstrate.
Expression of Tac antigen on activated normal human B cells. (1984)

Two-color fluorescence analysis revealed that Tac antigen, which was previously reported to be restricted to T cells, was expressed on a proportion of normal B cells activated by Staphylococcus aureus Cowan I (SAC). Immunoaffinity-purified interleukin 2 (IL-2) induced the proliferation of SAC-activated B cells, and the proliferation was completely inhibited by anti-Tac antibody, which blocked the membrane binding and action of IL-2. These results suggest that an IL-2 receptor system is directly involved in the B cell immune response.
Fetal-Maternal Conflict, Trophoblast Invasion, Preeclampsia, and the Red Queen (2008)

The much publicized conflict hypothesis for understanding fetal-maternal interaction during pregnancy often invokes a 'battle' metaphor, rather than a well orchestrated interplay occurring as a series of well controlled moves and counter-moves as happens in a game of chess. Such stepwise interaction is particularly obvious in the spiral artery remodelling process, and it would be interesting to trace the history of the successive steps in histological adaptation throughout primate phylogeny. The restricted invasion observed in a few species on a 'lower' evolutionary scale suggests a tendency of progressive deeper invasion during primate evolution. Unfortunately, our knowledge of invasive processes in the placental bed in nonhuman primates is highly inadequate. A paradigm underscoring the stepwise interaction between mother and fetus may be provided by the Red Queen hypothesis, which is a useful model to explain co-evolutionary processes between different species. The apparent association between preeclampsia and restricted endovascular trophoblast invasion, combined with the absence of the disease in primate species showing shallow invasion, suggests that preeclampsia may result from a failure in one or more interactive steps necessary for deeper invasion. Evidence for a genetic component invokes the puzzling question as to why "preeclampsia genes" are not eliminated from human populations. As in other fields of medicine, a proper understanding of Darwinian selection processes may throw some light on the causes of preeclampsia.