gebhart/patrepeval huggingface dataset:
Midkine, a Retinoic Acid-Inducible Heparin-Binding Cytokine in Inflammatory Responses: Chemotactic Activity to Neutrophils and Association with Inflammatory Synovitis (1997)

Midkine (MK) is a retinoic acid-inducible heparin-binding cytokine. In the inflammatory synovitis of rheumatoid arthritis and osteoarthritis, MK was detected in synovial fluid, synoviocytes, and endothelial cells of new blood vessels. Normal synovial fluid and noninflammatory synovial tissue did not contain detectable MK. Therefore, MK showed inflammation-associated expression in these cases. Furthermore, MK was found to promote chemotaxis of neutrophils in the range of 10 ng/ml. The mode of action of MK was found to be haptotactic; the substrate-bound form of MK was the active one. MK is also known to promote fibrinolysis. These activities of MK are in agreement with the modes of MK expression in various pathological statuses, and thus MK is proposed to be an important molecule regulating inflammatory responses.
Localisation of a mutation producing autosomal dominant polycystic kidney disease without renal failure. (1987)

A four generation Finnish family was identified with atypical features of adult polycystic kidney disease. All members of the extended pedigree were asymptomatic and none had developed renal failure. Previous studies have shown close linkage between the adult polycystic kidney disease locus and the alpha chain of human haemoglobin on chromosome 16, but these studies were carried out on families manifesting 'typical' clinical features of the disease. In order to determine whether the atypical clinical features observed in this Finnish family were produced by a mutation at the same or a second locus, linkage studies were carried out using a highly polymorphic DNA marker from the alpha globin cluster. Here we show that the mutation producing the disease in this Finnish family is also closely linked to alpha globin.
A new bone‐inducing biodegradable porous β‐tricalcium phosphate (2004)

A new type of degradable biomaterial with bone-inducing capacity was made by combining porous beta-tricalcium phosphate (beta-TCP) with a delivery system for recombinant human bone morphogenetic protein-2 (rhBMP-2). The BMP delivery system consisted of a block copolymer composed of poly-D,L-lactic acid with random insertion of p-dioxanone and polyethylene glycol (PLA-DX-PEG), a known biocompatible and biodegradable material. The efficacy of this biomaterial in terms of its bone-inducing capacity was examined by ectopic bone formation in the dorsal muscles of the mouse. In the beta-TCP implants coated with the PLA-DX-PEG polymer containing more than 0.0025% (w/w) of rhBMP-2, new ectopic bone tissues with marrow were consistently found on the surface of implants. The radiographic density of beta-TCP was diminished in a time-dependent manner. On histological examination, numerous multinucleated osteoclasts with positive tartrate-resistant acid-phosphatase (TRAP) staining were noted on the surface of the beta-TCP. These experimental results indicate that beta-TCP implants coated with synthetic rhBMP-2 delivery system might provide effective artificial bone-graft substitutes with osteoinductive capacity and biodegradable properties. In addition, this type of biomaterial may require less rhBMP-2 to induce significant new bone mass.
Periodic signals for measuring nonlinear Volterra kernels (1996)

The frequency-domain measurement of the Volterra kernels of a nonlinear system using periodic multisine signals is now a practical possibility. An analysis is presented of the harmonic output of a Volterra kernel when excited with a multiharmonic signal, which lays the basis for the design of such signals. This is followed by a review of previous work in this area, after which a range of new periodic signals is defined. The minimization of the signal crest factors is then examined, along with the practical problems associated with their application. Practical results are presented which illustrate the application of the signals to testing a reference nonlinear circuit and a servo motor system.
Functional Characterization and Localization of Acetyl-CoA Hydrolase, Ach1p, in Saccharomyces cerevisiae (2003)

Acetyl-CoA hydrolase (Ach1p), catalyzing the hydrolysis of acetyl-CoA, is presumably involved in regulating intracellular acetyl-CoA or CoASH pools; however, its intracellular functions and distribution remain to be established. Using site-directed mutagenesis analysis, we demonstrated that the enzymatic activity of Ach1p is dependent upon its putative acetyl-CoA binding sites. The ach1 mutant causes a growth defect in acetate but not in other non-fermentable carbon sources, suggesting that Ach1p is not involved in mitochondrial biogenesis. Overexpression of Ach1p, but not constructs containing acetyl-CoA binding site mutations, in ach1-1 complemented the defect of acetate utilization. By subcellular fractionation, most of the Ach1p in yeast was distributed with mitochondria and little Ach1p in the cytoplasm. By immunofluorescence microscopy, we show that Ach1p and acetyl-CoA binding site-mutated constructs, but not its N-terminal deleted construct, are localized in mitochondria. Moreover, the onset of pseudohyphal development in homozygote ach1-1 diploids was abolished. We infer that Ach1p may be involved in a novel acetyl-CoA biogenesis and/or acetate utilization in mitochondria and thereby indirectly affect pseudohyphal development in yeast.
Cig30, a Mouse Member of a Novel Membrane Protein Gene Family, Is Involved in the Recruitment of Brown Adipose Tissue (1997)

We have identified a previously uncharacterized gene that is implicated in the thermogenic function of brown adipose tissue of mice. This gene, termed Cig30, is the first mammalian member of a novel gene family comprising several nematode and yeast genes, such as SUR4 and FEN1, mutation of which is associated with highly pleiotropic phenotypes. It codes for a 30-kDa plasma membrane glycoprotein with five putative transmembrane domains. The Cig30 mRNA was readily detected only in brown fat and liver. When animals were exposed to a 3-day cold stress, the Cig30 expression was selectively elevated in brown fat more than 200-fold. Similar increases were brought about in two other conditions of brown fat recruitment, namely during perinatal development and after cafeteria diet. The magnitude of Cig30 mRNA induction in the cold could be mimicked by chronic norepinephrine treatment in vivo. However, in primary cultures of brown adipocytes, a synergistic action of norepinephrine and dexamethasone was required for full expression of the gene, indicating that both catecholamines and glucocorticoids are required for the induction of Cig30. We propose that the CIG30 protein is involved in a pathway connected with brown fat hyperplasia.
Boris I. Bittker: Colleague and Friend (1983)

Boris Bittker is not the kind to let University tradition or Connecticutstatute dictate the time of his retirement. His eminence as a scholar,teacher, and lawyer is due not only to his extraordinary gifts of mind andpersonality, but also to his hard work and self-discipline. It is thereforeentirely in keeping with his practice of controlling his own timetable thathe should have decided to retire last June, while in robust good health,several years ahead of the schedule ordained by others.
Hormones and the immunological capacity. 3. The immunodeficiency disease of the hypopituitary Snell-Bagg dwarf mouse. (1971)

The naturally occurring immunodeficiency syndrome of the hypopituitary Snell-Bagg dwarf mice has been characterized. The immunopathological aspects of this syndrome derive primarily from an arrested ontogenetic development of the thymus. The alteration of the thymus function is caused by the failure of the pituitary to produce certain hormones, especially somatotropic hormone. The relation of this syndrome of the dwarf mouse to human immunodeficiency diseases and endocrinopathies is discussed.
ANTIBODY SCREENING BY ENZYME-LINKED IMMUNOSORBENT ASSAY USING POOLED SOLUBLE HLA IN RENAL TRANSPLANT CANDIDATES (1997)

The enzyme-linked immunosorbent assay (ELISA) using HLA class I molecules purified from pooled platelets has the potential to detect HLA antibodies with increased efficiency without sacrificing sensitivity or specificity. This test, which was originally developed in our institution, has been independently validated by recent studies and is now commercially available. We now present evidence of its usefulness as a routine HLA antibody screening test for renal transplant patients. A total of 515 patients were tested monthly by ELISA (13.9 tests/patient) and by antiglobulin-enhanced panel reactivity (6.3 tests/patient). In patients found to be unsensitized, the incidence of false-positive results was less for ELISA than for the panel studies. In patients who were highly sensitized, both tests performed equally well, whereas discordant results were registered mainly in cases of mild sensitization. Because 66% of our patients were not sensitized, the ELISA was effective in reducing the number of more involved tests aimed at characterizing the antibodies. These results provide a foundation to use the pooled platelet HLA ELISA on a routine basis for HLA antibody screening.
Structure and Dynamics of 13C,15N‐Labeled Lipopolysaccharides in a Membrane Mimetic (2008)

The natural look of endotoxin: A new approach has been developed to determine the chemical structure, dynamics, and conformation of lipopolysaccharide (endotoxin, see structure) by solution NMR spectroscopy. Using isotope labeling and solubilization by dihexanoylphosphatidylcholine as a mimetic of the bacterial membrane, a general approach makes complex endotoxin molecules amenable to analysis by heteronuclear NMR methods. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Differential regulation of the endoplasmic reticulum stress response in pancreatic β-cells exposed to long-chain saturated and monounsaturated fatty acids (2008)

Exposure of pancreatic beta-cells to long-chain fatty acids leads to the activation of some components of the endoplasmic reticulum (ER) stress pathway and this mechanism may underlie the ability of certain fatty acids to promote beta-cell death. We have studied ER stress in BRIN-BD11 beta-cells exposed to either the saturated fatty acid palmitate (C16:0) or the monounsaturated palmitoleate (C16:1). Palmitate (0.025-0.25 mM) induced the expression of various markers of the RNA-dependent protein kinase-like ER eukaryotic initiation factor 2 alpha (eIF2 alpha) kinase (PERK)-dependent pathway of ER stress (phospho-eIF2 alpha; ATF4, activating transcription factor 4 and C/EBP homologous protein (CHOP-10)) although it failed to promote the expression of the ER chaperone GRP78. By contrast, palmitoleate did not induce any markers of the ER stress pathway even at concentrations as high as 1 mM. When palmitate and palmitoleate were added in combination, a marked attenuation of the ER stress response occurred. Under these conditions, the levels of phospho-eIF2 alpha, ATF4 and CHOP-10 were reduced to less than those found in control cells. Palmitoleate also attenuated the ER stress response to the protein glycosylation inhibitor, tunicamycin, and improved the viability of the cells exposed to this agent. Exposure of the BRIN-BD11 cells to the protein phosphatase inhibitor, salubrinal, in the absence of fatty acids resulted in increased eIF2 alpha phosphorylation but this was abolished by co-incubation with palmitoleate. We conclude that saturated fatty acids activate components of the PERK-dependent ER stress pathway in beta-cells, ultimately leading to increased apoptosis. This effect is antagonised by monounsaturates that may exert their anti-apoptotic actions by regulating the activity of one or more kinase enzymes involved in mediating the phosphorylation of eIF2 alpha.
From Cairo to Beijing and beyond. Women's rights in context. (1996)

The Fourth World Conference on Women was the largest conference in the history of the UN, drawing 50,000 people to Beijing and surrounding areas during the late Summer. The final Platform for Action garnered broad support among women's health advocates and feminists. Indeed, for feminists, the document reflected far more progress than anticipated on controversial questions related to human rights, sexuality, and reproductive health and rights. The 345-paragraph Platform for Action is expected to serve as a blueprint for advocacy by nongovernmental organizations supporting women's rights and as a basis for policy change and resource commitment by most of the world's governments. Although approximately 40 nations had reservations on controversial aspects of the Platform, most of the approximately 180 countries participating associated themselves with all provisions of the document. The road to Beijing, the Platform for Action, the 1994 International Conference on Population and Development, sexual rights, and the need to continue the struggle against religious conservatives and other related opposition are discussed.
Atypical Mowat-Wilson patient confirms the importance of the novel association between ZFHX1B/SIP1 and NuRD corepressor complex (2008)

Mutations in ZFHX1B cause Mowat-Wilson syndrome (MWS) but the precise mechanisms underlying the aberrant functions of mutant ZFHX1B proteins (also named Smad-interacting protein-1, SIP1) in patients are unknown. Using mass spectrometry analysis, we identified subunits of the NuRD corepressor complex in affinity-purified Zfhx1b complexes. We find that Zfhx1b associates with NuRD through its N-terminal domain, which contains a previously postulated NuRD interacting motif. Interestingly, this motif is substituted by an unrelated sequence in a recently described MWS patient. We show here that such aberrant ZFHX1B protein is unable to recruit NuRD subunits and displays reduced transcriptional repression activity on the XBMP4 gene promoter, a target of Zfhx1b. We further demonstrate that the NuRD component Mi-2beta is involved in repression of the Zfhx1b target gene E-cadherin as well as in Zfhx1b-induced neural induction in animal caps from Xenopus embryos. Thus, NuRD and Zfhx1b functionally interact, and defective NuRD recruitment by mutant human ZFHX1B can be a MWS-causing mechanism. This is the first study providing mechanistic insight into the aberrant function of a single domain of the multi-domain protein ZFHX1B/SIP1 in human disease.
Association of E-cadherin and beta-catenin with metastasis in nasopharyngeal carcinoma. (2004)

The results suggest that the downregulation of E-cadherin results from the gene promoter aberrant methylation of E-cadherin and that the methylation of E-cadherin plays an important role in invasion and metastasis of tumor cells in NPC. However, beta-catenin mutation is an infrequent event in NPC, and beta-catenin is not a critical factor influencing the invasion and metastasis of tumor cells in NPC.
Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene. (1994)

The agouti coat color gene encodes a paracrine signaling molecule that controls the production of yellow and black pigment by melanocytes within hair follicles. Some agouti alleles affect the dorsum and ventrum independently, which has provided the basis for speculation that agouti gene action in different regions of the body is controlled by distinct genetic loci that are closely linked. Using a combination of cDNA cloning and RNA expression studies, we find that alternative isoforms of agouti mRNA contain different noncoding first exons located 100 kb apart, whose patterns of expression indicate independent control by regulatory elements that are either ventral specific or hair cycle specific. These results demonstrate that the apparent genetic complexity of the agouti locus is explained by the existence of multiple regulatory elements exerting control over a single coding sequence and provide a conceptual basis for understanding differences in dorsal and ventral hair coloration in many mammalian species. The ventral-specific agouti isoform represents an example of a transcript whose expression is restricted to ventral skin and provide an approach to investigate the mechanisms by which dorsal-ventral differences in gene expression are established and maintained.
Dietary Phosphorus Transcriptionally Regulates 25-Hydroxyvitamin D-1α-Hydroxylase Gene Expression in the Proximal Renal Tubule (2002)

Synthesis of the hormone 1,25-dihydroxyvitamin D, the biologically active form of vitamin D, occurs in the kidney and is catalyzed by the mitochondrial cytochrome P450 enzyme, 25-hydroxyvitamin D-1alpha-hydroxylase (1alpha-hydroxylase). We sought to characterize the effects of changes in dietary phosphorus on the kinetics of renal mitochondrial 1alpha-hydroxylase activity and the renal expression of P450c1alpha and P450c24 mRNA, to localize the nephron segments involved in such regulation, and to determine whether transcriptional mechanisms are involved. In intact mice, restriction of dietary phosphorus induced rapid, sustained, approximately 6- to 8-fold increases in renal mitochondrial 1alpha-hydroxylase activity and renal P450c1alpha mRNA abundance. Immunohistochemical analysis of renal sections from mice fed the control diet revealed the expression of 1alpha-hydroxylase protein in the proximal convoluted and straight tubules, epithelial cells of Bowman's capsule, thick ascending limb of Henle's loop, distal tubule, and collecting duct. In mice fed a phosphorus-restricted diet, immunoreactivity was significantly increased in the proximal convoluted and proximal straight tubules and epithelial cells of Bowman's capsule, but not in the distal nephron. Dietary phosphorus restriction induced a 2-fold increase in P450c1alpha gene transcription, as shown by nuclear run-on assays. Thus, the increase in renal synthesis of 1,25-dihydroxyvitamin D induced in normal mice by restricting dietary phosphorus can be attributed to an increase in the renal abundance of P450c1alpha mRNA and protein. The increase in P450c1alpha gene expression, which occurs exclusively in the proximal renal tubule, is due at least in part to increased transcription of the P450c1alpha gene.
Bioinspired Design of a Superoleophobic and Low Adhesive Water/Solid Interface (2008)

The wetting/antiwetting behavior of liquid droplets on a solid surface is not an apparent or simple contact between two phases, but among three phases. Inspired by the antiwetting behavior of oil droplets on fish scales in water, a superoleophobic and low-adhesive interface is created on a solid substrate with micro/nanohierarchical structures, using oil/water/solid three-phase systems.
Occipitocervical Fusion for Rheumatoid Arthritis Using the Inside–Outside Stabilization Technique (2003)

The "inside-outside" technique is safe and effective for stabilizing the occipitocervical junction in rheumatoid patients. It is associated with significant reduction of neck pain, improved neurologic status, and maintenance of alignment and stability.
Reflections on the Readings of Sundays and Feasts February - April (2006)

A reflection on the readings of Sundays and feasts during February 2006 and April 2006 is presented.
Preparation of the High-Tc Phase of Bi-Sr-Ca-Cu-O Superconductor (1988)

Co-decomposition of mixed nitrates of Bi, Pb, Sr, Ca and Cu around 830°C under low oxygen pressure led to the formation of a high- T c superconducting phase of Bi(Pb)-Sr-Ca-Cu-O with T c (zero) at 107.5 K. A sample prepared by a conventional solid state reaction method under low oxygen pressure also showed the superconducting transition at 107.5 K. X-ray powder diffraction and magnetic susceptibility measurements on these samples revealed the high- T c phase without 80 K or semiconducting phase. The reaction under low oxygen pressure has an effect to lower the temperature with broad ranges to render the high- T c phase of the Bi-Sr-Ca-Cu-O.
Kinetics of II-VI and III-V Colloidal Semiconductor Nanocrystal Growth:  “Focusing” of Size Distributions (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTKinetics of II-VI and III-V Colloidal Semiconductor Nanocrystal Growth: "Focusing" of Size DistributionsXiaogang Peng, J. Wickham, and A. P. AlivisatosView Author Information Department of Chemistry, University of California Berkeley and Materials Sciences Division Lawrence Berkeley National Lab Berkeley, California 94720-1460 Cite this: J. Am. Chem. Soc. 1998, 120, 21, 5343–5344Publication Date (Web):May 14, 1998Publication History Received18 February 1998Published online14 May 1998Published inissue 1 June 1998https://pubs.acs.org/doi/10.1021/ja9805425https://doi.org/10.1021/ja9805425rapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views22646Altmetric-Citations1691LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cadmium selenide,Crystallization,Monomers,Nanocrystals,Nanoparticles Get e-Alerts
ALMOST P-SPACES (2003)

In this paper, we have characterizations of almost P-spaces which are analogous characterizations of P-spaces and we will show that if X is an almost P-space such that it is <TEX>$C^{*}-embedded$</TEX> in every almost P-space in which X is embedded, then <TEX>$<TEX>$\mid$</TEX>{\upsilon}X-X<TEX>$\mid$</TEX>{\leq}1$</TEX> and that if <TEX>$<TEX>$\mid$</TEX>{\upsilon}X-X<TEX>$\mid$</TEX>{\leq}1$</TEX> and <TEX>${\upsilon}X$</TEX> is Lindelof, then for any almost P-space Y in which X is dense embedded, then X is <TEX>$C^{*}-embeded$</TEX> in Y.
Do interhelical side chain-backbone hydrogen bonds participate in formation of leucine zipper coiled coils? (1991)

The leucine zipper proteins are a group of transcriptional regulators that dimerize to form a DNA binding domain. It has been proposed that this dimerization results from the hydrophobic association of the alpha-helices of two leucine zipper monomers into a coiled coil. We propose a model for a coiled coil based on a periodic hydrophobic-hydrophilic amino acid motif found in the leucine zipper regions of 11 transcriptional regulatory proteins. This model predicts the symmetrical formation of secondary hydrogen bonds between the polar side chains of one helix and the peptide carbonyls of the opposite chain, supplementing the interactions between hydrophobic side chains. Physical modeling (CPK) and in vacuo molecular mechanics calculations of the stability of the GCN4 leucine zipper coiled coil configured in accordance with this model demonstrate a greater stability for this conformer than for a conformer configured according to a current hydrophobic model. Molecular dynamics simulations show similar stability of the two models in vacuo but a higher stability of the hydrophobic model in water.