gebhart/patrepeval huggingface dataset:
Native albumin for targeted drug delivery (2010)

Native albumin is an effective drug carrier to sites of inflammation or malignancy.
Comparative transcriptomics of 5 high-altitude vertebrates and their low-altitude relatives (2017)

These data serve as a valuable resource for examining the convergence and divergence of gene expression changes between species as they adapt or acclimatize to high-altitude environments.
Physically Gelled Ionic Liquids: Solid Membrane Materials with Liquidlike CO2 Gas Transport (2009)

A room-temperature ionic liquid was combined with a low-molecular-weight organic gelator to form a cuttable, soft gel solid. When used as a supported membrane, this material has enhanced transport properties for CO2 gas separations compared to traditional supported liquid membranes, while showing increased mechanical stability.
Use of plant roots for phytoremediation and molecular farming (1999)

Alternative agriculture, which expands the uses of plants well beyond food and fiber, is beginning to change plant biology. Two plant-based biotechnologies were recently developed that take advantage of the ability of plant roots to absorb or secrete various substances. They are (i) phytoextraction, the use of plants to remove pollutants from the environment and (ii) rhizosecretion, a subset of molecular farming, designed to produce and secrete valuable natural products and recombinant proteins from roots. Here we discuss recent advances in these technologies and assess their potential in soil remediation, drug discovery, and molecular farming.
The Synthesis and Solution Properties of Aromatic Polymers Containing 2,4-Quinoline Units in the Main Chain (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Synthesis and Solution Properties of Aromatic Polymers Containing 2,4-Quinoline Units in the Main ChainJ. F. Wolfe and J. K. StilleCite this: Macromolecules 1976, 9, 3, 489–496Publication Date (Print):May 1, 1976Publication History Published online1 May 2002Published inissue 1 May 1976https://pubs.acs.org/doi/10.1021/ma60051a020https://doi.org/10.1021/ma60051a020research-articleACS PublicationsRequest reuse permissionsArticle Views228Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Some Experiments on the Effect of Contact Angle in Agglomeration from Liquids (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSome Experiments on the Effect of Contact Angle in Agglomeration from LiquidsYoshiaki Kawashima and C. Edward CapesCite this: Ind. Eng. Chem. Fundamen. 1980, 19, 3, 312–314Publication Date (Print):August 1, 1980Publication History Published online1 May 2002Published inissue 1 August 1980https://pubs.acs.org/doi/10.1021/i160075a014https://doi.org/10.1021/i160075a014research-articleACS PublicationsRequest reuse permissionsArticle Views92Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Structure and evolutionary origin of the gene encoding a human serum mannose-binding protein (1989)

The N-terminal sequence of the major human serum mannose-binding protein (MBP1) was shown to be identical at all positions determined with the amino acid sequence predicted from a cDNA clone of a human liver MBP mRNA. An oligonucleotide corresponding to part of the sequence of this cDNA clone was used to isolate a cosmid genomic clone containing a homologous gene. The intron/exon structure of this gene was found to closely resemble that of the gene encoding a rat liver MBP (MBP A). The nucleotide sequence of the exons differed in several places from that of the human cDNA clone published by Ezekowitz, Day & Herman [(1988) J. Exp. Med. 167, 1034-1046]. The MBP molecule comprises a signal peptide, a cysteine-rich domain, a collagen-like domain, a 'neck' region and a carbohydrate-binding domain. Each domain is encoded by a separate exon. This genomic organization lends support to the hypothesis that the gene arose during evolution by a process of exon shuffling. Several consensus sequences that may be involved in controlling the expression of human serum MBP have been identified in the promoter region of the gene. The consensus sequences are consistent with the suggestion that this mammalian serum lectin is regulated as an acute-phase protein synthesized by the liver.
EDGAR 2.0: an enhanced software platform for comparative gene content analyses (2016)

The rapidly increasing availability of microbial genome sequences has led to a growing demand for bioinformatics software tools that support the functional analysis based on the comparison of closely related genomes. By utilizing comparative approaches on gene level it is possible to gain insights into the core genes which represent the set of shared features for a set of organisms under study. Vice versa singleton genes can be identified to elucidate the specific properties of an individual genome. Since initial publication, the EDGAR platform has become one of the most established software tools in the field of comparative genomics. Over the last years, the software has been continuously improved and a large number of new analysis features have been added. For the new version, EDGAR 2.0, the gene orthology estimation approach was newly designed and completely re-implemented. Among other new features, EDGAR 2.0 provides extended phylogenetic analysis features like AAI (Average Amino Acid Identity) and ANI (Average Nucleotide Identity) matrices, genome set size statistics and modernized visualizations like interactive synteny plots or Venn diagrams. Thereby, the software supports a quick and user-friendly survey of evolutionary relationships between microbial genomes and simplifies the process of obtaining new biological insights into their differential gene content. All features are offered to the scientific community via a web-based and therefore platform-independent user interface, which allows easy browsing of precomputed datasets. The web server is accessible at http://edgar.computational.bio.
Chimeric Live, Attenuated Vaccine against Japanese Encephalitis (ChimeriVax‐JE): Phase 2 Clinical Trials for Safety and Immunogenicity, Effect of Vaccine Dose and Schedule, and Memory Response to Challenge with Inactivated Japanese Encephalitis Antigen (2003)

ChimeriVax-JE is a live, attenuated vaccine against Japanese encephalitis, using yellow fever (YF) 17D vaccine as a vector. In a double-blind phase 2 trial, 99 adults received vaccine, placebo, or YF 17D vaccine (YF-VAX). ChimeriVax-JE was well tolerated, with no differences in adverse events between treatment groups. Viremias resulting from administration of ChimeriVax-JE and YF-VAX were of short duration and low titer; 82 (94%) of 87 subjects administered graded doses (1.8-5.8 log(10)) of ChimeriVax-JE developed neutralizing antibodies. A second dose, administered 30 days later, had no booster effect. Previous inoculation with YF did not interfere with ChimeriVax-JE, but there was a suggestion (not statistically significant) that ChimeriVax-JE interfered with YF-VAX administered 30 days later. A separate study explored immunological memory both in subjects who had received ChimeriVax-JE 9 months before and in ChimeriVax-JE-naive subjects challenged with inactivated mouse-brain vaccine (JE-VAX). Anamnestic responses were observed in preimmune individuals. ChimeriVax-JE appears to be a safe vaccine that provides protective levels of neutralizing antibody after a single dose.
A high performance transceiver hybrid IC for PHS hand-set operating with single positive voltage supply (2002)

A high performance transceiver hybrid IC (HIC) operating with single positive voltage supply has been developed for PHS hand-set. The HIC integrates a power amplifier (PA), a front-end (F/E) IC, a T/R SPDT switch (SW) and their peripheral circuits, which are mounted on the HIC circuit board with thick Cu metal heat-sink. In the transmitter block, the PA with a SW exhibits a low dissipation current of 190 mA, a high power gain of 39.4 dB and a low adjacent channel leakage power of -56.4 dBc at an output power of 20.5 dBm. In the receiver block, the F/E with a SW exhibits a low noise figure of 3.7 dB, a low dissipation current of 4.8 mA and a high conversion gain of 25.4 dB. The HIC needs only single positive voltage supply of 3.5 V. The HIC size is only 10 mm/spl times/13 mm/spl times/1.9 mm.
The flow deviation method: An approach to store‐and‐forward communication network design (1973)

Abstract Two problems relevant to the design of a store‐and‐forward communication network (the message routing problem and the channel capacity assignment problem) are formulated and are recognized to be essentially non‐linear, unconstrained multicommodity (m.c.) flow problems. A “Flow Deviation” (FD) method for the solution of these non‐linear, unconstrained m.c. flow problems is described which is quite similar to the gradient method for functions of continuous variables; here the concept of gradient is replaced by the concept of “shortest route” flow. As in the gradient method, the application of successive flow deviations leads to local minima. Finally, two interesting applications of the FD method to the design of the ARPA Computer Network are discussed.
Do shock absorbing insoles in recruits undertaking high levels of physical activity reduce lower limb injury? A randomized controlled trial (2006)

Objectives To assess the benefits, if any, of the use of shock absorbing insoles in reducing lower limb injury among Air Force recruits, and to assess the differences, if any, in the efficacy of two commonly available shock absorbing insoles. Design Randomized controlled trial. Setting: RAF Halton, UK. Site of all basic training for RAF personnel. Participants: 1205 recruits participating in basic training between 17 September 2003 and 7 April 2004. Interventions: Participants were randomized to receive either standard issue Saran non-shock absorbing insoles, or shock absorbing Sorbothane or Poron insoles, on a 1:1:1 basis. Main Outcome Measures The primary outcome measure was withdrawal from training for lower limb injury. The two primary comparisons were shock absorbing insole versus non-shock absorbing insole, and Sorbothane versus Poron (comparison of different shock absorbing insoles). Secondary outcomes were medical withdrawals for reasons other than those qualifying for the primary outcome measure. Results When comparing the non-shock absorbing insole to the shock absorbing insoles 72/401 participants (18.0%) allocated to Saran insoles were removed from training because of a qualifying lower limb injury, compared with 149/ 804 (18.5%) allocated to the shock absorbing insole (Sorbothane or Poron), odds ratio 1.04 (95% CI 0.75 to 1.44; P=0.87). When comparing the two shock absorbing insole 73/ 421 participants (17.3%) randomized to Sorbothane were removed from training because of a qualifying lower limb injury, compared with 76/383 for Poron (19.8%), odds ratio 0.85 (95% CI 0.58 to 1.23; P=0.37). Conclusions Similar rates of lower limb injuries were observed for all insoles (shock absorbing and non-shock absorbing) in the trial. The trial provides no support for a change in policy to the use of shock absorbing insoles for military recruits.
Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant. (1992)

Polysome-associated c-myc mRNA is degraded relatively rapidly in cells and in an in vitro mRNA decay system containing extracts from cultured mammalian cells. Using this system, a competition/screening assay was devised to search for factors that bind to specific regions of polysome-associated c-myc mRNA and thereby alter its half-life. mRNA stability was first assayed in reactions containing exogenous competitor RNAs corresponding to portions of c-myc mRNA itself. The addition of a 182-nucleotide sense strand fragment from the carboxy-terminal portion of the c-myc-coding region destabilized c-myc mRNA by at least eightfold. This RNA fragment had no effect on the stability of other mRNAs tested. Moreover, c-myc mRNA was not destabilized in reactions containing unrelated competitor RNAs or sense strand RNA from the c-myc 5' region. Polysome-associated globin mRNA containing the c-myc-coding region segment in-frame was also destabilized in vitro by the 182-nucleotide RNA. As determined by UV-cross-linking experiments, the 182-nucleotide RNA fragment was recognized by and bound to an approximately 75-kD polysome-associated protein. On the basis of these data plus Northern blotting analyses of c-myc mRNA decay products, we suggest that the approximately 75-kD protein is normally bound to a c-myc-coding region determinant and protects that region of the mRNA from endonuclease attack. Possible links between the protective protein, translation, ribosome pausing, and c-myc mRNA turnover are discussed.
Regulation of N-Linked Core-Glycosylation. (1996)

N型グリコシル化は、最も一般的なタンパク質の修飾機構の一つであり、タンパク質として合成され機能を発現する上で大変重要である。この修飾のタンパク質の機能に及ぼす影響は、N型糖鎖修飾が起こる時に、タンパク質に付加される糖鎖の本数やその位置で異なる。Asn-X-Ser/Thr配列は、タンパク質に糖鎖修飾が起こるためのシグナルであるが、必ずしもAsn-X-Ser/Thr配列が効率的にグリコシル化を受けるわけでもなく、またこの配列に全く糖鎖が結合していない場合もあり、他のタンパク質シグナルも関与していると考えられる。あるAsn残基に起こる糖鎖修飾を制御しているシグナルを明らかにするため、様々な実験が行なわれてきた。例えば、糖鎖が結合している配列としていない配列の近傍のタンパク質の一次構造を比較したり、糖鎖結合可能部位を含むペプチドをオリゴ糖の受容体や阻害剤として用いたり、また組換え体タンパク質の糖鎖結合可能部位に糖鎖が結合しているかどうかを解析した研究がある。こうした研究によって、様々な因子が複雑に組合わさることにより、個々の Asn-X-Ser/Thr配列にオリゴ糖が付加される効率が決まることが明らかとなった。糖鎖結合可能部位のAsn残基近傍のアミノ酸は、オリゴ糖の受容体活性に大きな影響を与えている可能性がある。これ以外の因子は、タンパク質が合成されている際のグリコシル化が起こる瞬間に、糖鎖結合可能部位が糖鎖修飾を受けやすいようにすることで、その効率を調節している。本稿では、こうした問題を取り扱っている研究について議論してみたい。
Investigation of Optimal Specimen Type and Sampling Time for Detection of Measles Virus RNA during a Measles Epidemic (2001)

At various times postonset of rash, 74 patients positive for measles virus-specific immunoglobulin M provided samples for detection of measles virus RNA by a reverse transcriptase PCR. Of lymphocytes, urine, throat swab, and serum specimens, throat swab specimens were optimal for detection of measles virus RNA during the first 2 weeks after the rash.
Strain-Sensing Characteristics of Carbon Fiber-Reinforced Cement (2005)

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Practical methods for synthesizing four incompletely condensed silsesquioxanes from a single R8Si8O12 framework (1998)

The reaction of (c-C6H11)8Si8O12 1 with triflic acid (TfOH) can produce two different ditriflates with the formula (c-C6H11)8Si8O11(OTf)2, which can be hydrolyzed selectively to four different incompletely condensed silsesquioxane frameworks with the formula (c-C6H11)8Si8O11(OH)2 (2, 4, 7 and 8).
Requirement of hypoxia-inducible factor-1α down-regulation in mediating the antitumor activity of the anti–epidermal growth factor receptor monoclonal antibody cetuximab (2008)

We tested our novel hypothesis that down-regulation of hypoxia-inducible factor-1alpha (HIF-1alpha), the regulated subunit of HIF-1 transcription factor that controls gene expression involved in key functional properties of cancer cells (including metabolism, survival, proliferation, invasion, angiogenesis, and metastasis), contributes to a major antitumor mechanism of cetuximab, an approved therapeutic monoclonal antibody that blocks activation of the epidermal growth factor receptor. We showed that cetuximab treatment down-regulates HIF-1alpha levels by inhibiting synthesis of HIF-1alpha rather than by enhancing degradation of the protein. Inhibition of HIF-1alpha protein synthesis was dependent on effective inhibition of the phosphoinositide-3 kinase (PI3K)/Akt pathway by cetuximab, because the inhibition was prevented in cells transfected with a constitutively active PI3K or a constitutively active Akt but not in cells with a constitutively active MEK. Overexpression of HIF-1alpha conferred cellular resistance to cetuximab-induced apoptosis and inhibition of vascular endothelial growth factor production in sensitive cancer cell models, and expression knockdown of HIF-1alpha by RNA interference substantially restored cellular sensitivity to the cetuximab-mediated antitumor activities in experimental resistant cell models created by transfection of an oncogenic Ras gene (G12V) or by concurrent treatment of the cells with insulin-like growth factor-I. In summary, our data show that cetuximab decreases HIF-1alpha protein synthesis through inhibition of a PI3K-dependent pathway and that an effective down-regulation of HIF-1alpha is required for maximal therapeutic effects of cetuximab in cancer cells.
Impact of a Protocol for Acute Antifibrinolytic Therapy on Aneurysm Rebleeding After Subarachnoid Hemorrhage (2008)

When used acutely, short-term EACA treatment resulted in decreased rebleeding without an increase in serious side effects in our selected group of patients. Randomized placebo-controlled trials are needed to determine whether acute antifibrinolytic therapy should be accepted as the standard of care in all patients.
Interactions of plasmid-encoded replication initiation proteins with the origin of DNA replication in the broad host range plasmid RK2 (1991)

The TrfA proteins, encoded by the broad host range plasmid RK2, are required for replication of this plasmid in a variety of Gram-negative bacteria. Two TrfA proteins, 33 and 44 kDa in molecular mass (designated TrfA-33 and TrfA-44, respectively), are expressed from the trfA gene of RK2 through the use of two alternative in-frame start codons within the same open reading frame. The two proteins have been purified from Escherichia coli to near homogeneity as a mixture of wild-type TrfA-44/33, as TrfA-33 alone and as a functional variant form of TrfA-44, designated TrfA-44(98L), which contains a leucine in place of the TrfA-33 methionine start codon. Cross-linking experiments demonstrated that TrfA-33 can multimerize in solution. By using gel mobility shift and DNase I footprinting techniques the binding properties of TrfA-33, TrfA-44(98L), and TrfA-44/33 to the origin of replication of plasmid RK2 were analyzed. All three protein preparations were able to bind very specifically to the cluster of five direct repeats (iterons) contained in the minimal origin of replication. Each protein preparation produced a ladder of TrfA/minimal oriV complexes of decreasing electrophoretic mobility. The DNase I protection pattern on the five iterons was identical for all three protein preparations and extended from the beginning of the first iteron to 5 base pairs upstream of the fifth iteron. Studies on the affinity of the proteins for DNA fragments containing one, two, or all five iterons of the origin revealed a strong preference of TrfA protein for DNA containing at least two iterons. To study the stability of TrfA.DNA complexes, association and dissociation rates of TrfA-33 and DNA fragments with one, two, or five iterons were measured. This analysis showed that unlike complexes involving two or five iterons the TrfA/one iteron complexes were highly unstable, suggesting some form of cooperativity between proteins or iterons in the formation of stable complexes and/or the requirement of specific sequences bordering the iterons at the RK2 origin of replication for the stabilization of TrfA/DNA complexes.
The pattern of gene expression in human CD15+myeloid progenitor cells (2001)

We performed a genome-wide analysis of gene expression in primary human CD15(+) myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. The correct genes were confirmed by application of the generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) technique for high-copy tags with multiple matches. A set of genes known to be important in myeloid differentiation were expressed at various levels and used different spliced forms. This study provides a normal baseline for comparison of gene expression in myeloid diseases. The strategy of using SAGE and GLGI techniques in this study has broad applications to the genome-wide identification of expressed genes.
A NOVEL FEATURE EXTRACTION TECHNIQUE FOR FACIAL EXPRESSION RECOGNITION (2013)

This paper presents a new technique to extract the light invariant local feature for facial expression recognition. It is not only robust to monotonic gray-scale changes caused by light variations but also very simple to perform which makes it possible for analyzing images in challenging real-time settings. The local feature for a pixel is computed by finding the direction of the neighboring of the pixel with the particular rank in term of its gray scale value among all the neighboring pixels. When eight neighboring pixels are considered, the direction of the neighboring pixel with the second minima of the gray scale intensity can yield the best performance for the facial expression recognition in our experiment. The facial expression classification in the experiment was performed using a support vector machine on CK+ dataset The average recognition rate achieved is 90.1 ± 3.8%, which is better than other previous local feature based methods for facial expression analysis. The experimental results do show that the proposed feature extraction technique is fast, accurate and efficient for facial expression recognition.
Rapid Saccular Aneurysm Induction by Elastase Application in Vitro (1997)

After empirically testing several methods of elastase delivery, we were able to induce saccular, bifurcation-type aneurysms in animal arterial specimens. These aneurysms are histologically similar and more authentic than surgical models. The procedure is easy and reproducible. Our results suggest a possible enzymatic role in aneurysm formation and highlight the dramatic effects of selective arterial elastic damage. Also, the rapid growth of our experimental aneurysms may reflect the speed of the natural process.