gebhart/patrepeval huggingface dataset:
Signaling function of reconstituted CD16: ζ: γ receptor complex isoforms (1992)

Natural killer cells express an Fc receptor for IgG (CD16) in association with disulfide-linked dimers composed of two homologous subunits: the zeta chain of the T cell antigen receptor complex and the gamma chain of the mast cell/basophil Fc receptor for IgE. The ability of zeta and gamma to transduce CD16-mediated activation signals was compared by reconstituting distinct CD16 receptor isoforms composed of various combinations of zeta- and gamma-containing dimers. Stably transformed non-hematopoietic and hematopoietic cell lines were established that expressed chimeric molecules comprising the extracellular domain of CD16 joined to the transmembrane and intracellular domains of zeta or gamma. Reconstituted CD16 receptor complexes triggered Ca2+ influx, tyrosine phosphorylation, and IL-2 production in stable transformants of the Jurkat T cell line. However, cross-linking of the CD16/gamma chimera induced a specific pattern of tyrosine phosphorylation and was more efficient at signal transduction than a CD16, zeta-zeta complex, suggesting that zeta and gamma cytoplasmic domains may be coupled to distinct tyrosine kinase pathways that differentially regulate CD16-mediated activation signals. By contrast, both CD16/zeta and CD16/gamma chimeric molecules were not functional in stable transformants of the fibroblast Chinese Hamster Ovary cell line, indicating a requirement for downstream signaling components present in hematopoietic cells. Finally, the zeta transmembrane domain appears to preferentially associate with CD16 rather than the CD3:TCR complex, suggesting that a hierarchy of molecular interactions governs NK and T cell differentiation.
Acoustophoretic Control of Microparticle Transport Using Dual-Wavelength Surface Acoustic Wave Devices (2019)

We present a numerical and experimental study of acoustophoretic manipulation in a microfluidic channel using dual-wavelength standing surface acoustic waves (SSAWs) to transport microparticles into different outlets. The SSAW fields were excited by interdigital transducers (IDTs) composed of two different pitches connected in parallel and series on a lithium niobate substrate such that it yielded spatially superimposed and separated dual-wavelength SSAWs, respectively. SSAWs of a singltablee target wavelength can be efficiently excited by giving an RF voltage of frequency determined by the ratio of the velocity of the SAW to the target IDT pitch (i.e., <i>f</i> = <i>c</i><sub>SAW</sub>/<i>p</i>). However, the two-pitch IDTs with similar pitches excite, less efficiently, non-target SSAWs with the wavelength associated with the non-target pitch in addition to target SSAWs by giving the target single-frequency RF voltage. As a result, dual-wavelength SSAWs can be formed. Simulated results revealed variations of acoustic pressure fields induced by the dual-wavelength SSAWs and corresponding influences on the particle motion. The acoustic radiation force in the acoustic pressure field was calculated to pinpoint zero-force positions and simulate particle motion trajectories. Then, dual-wavelength SSAW acoustofluidic devices were fabricated in accordance with the simulation results to experimentally demonstrate switching of SSAW fields as a means of transporting particles. The effects of non-target SSAWs on pre-actuating particles were predicted and observed. The study provides the design considerations needed for the fabrication of acoustofluidic devices with IDT-excited multi-wavelength SSAWs for acoustophoresis of microparticles.
Prevention of the Hemodynamic Effects of Iopromide-Carrying Liposomes in Rats and Pigs (2000)

Allergy-like side effects induced by liposome injection strongly depend on the size, electric charge, and composition of the particles. The mechanism triggered by liposome injection probably is complex and can be effectively blocked by pretreatment with acetylsalicylic acid.
Effect of hydrogen loading on temperature/electric-fieldpoling of SiO 2 -based thin filmson Si (1995)

Waveguide stacks of Ge:SiO2-based glass deposited on Si exhibit a permanent second-order nonlinearity following temperature/electric-field poling. High temperature hydrogen loading increases the second harmonic signal under certain conditions.
Differences in side effects of amantadine hydrochloride and rimantadine hydrochloride relate to differences in pharmacokinetics (1983)

In a double-blind, placebo-controlled study, the comparative toxicities and blood concentrations of amantadine hydrochloride and rimantadine hydrochloride were determined. Healthy, working adults ingested either 200 (n = 52) or 300 mg (n = 196) per day in divided doses for 4.5 days. Mean plasma drug concentrations at 4 h after the first dose were lower in rimantadine recipients given 100- (140 versus 300 ng/ml for rimantadine and amantadine, respectively; P less than 10(-5)) or 200-mg doses (310 versus 633 ng/ml; P less than 10(-5)). The plasma drug concentrations after the first dose correlated significantly with total symptom sources for both amantadine and rimantadine, but the plasma levels of toxic and nontoxic subjects overlapped extensively. At 300-mg/day dosage amantadine was associated more often with adverse central nervous system symptoms (33% of amantadine versus 9% of rimantadine recipients; P less than 0.001) and sleep disturbance (39 versus 13%; P less than 0.001), but not gastrointestinal symptoms (19.5 versus 16.0%). However, no differences between the drugs were noted in symptom frequency or scores in volunteers with similar plasma concentrations. Amantadine and rimantadine differ in their pharmacokinetics but not in their potential for side effects at comparable plasma concentrations.
A MONOCLONAL ANTIBODY DETECTING A NEW HUMAN T CELL ANTIGEN, HULY-M2 (1984)

HuLy-m2 is a new T cell antigen detected by a monoclonal antibody. The antibody (anti-HuLy-m2) reacts with 85% of human T cells, 20% B cells, and 50% null cells in peripheral blood. The antibody is cytotoxic, binds protein A, and is of the IgG2a subclass. HuLy-m2 antigen consists of a glycoprotein dimer Mr 37,000 subunit structure, in which sialic acid and carbohydrate play some role in antigenicity. Capping studies showed the HuLy-m2 antigen to be distinct from the previously described OKT-3, 4, 8, HuLy-m1 (OKT11), and HuLy-m3 antigens.
Synthesis, Comparative Photosensitizing Efficacy, Human Serum Albumin (Site II) Binding Ability, and Intracellular Localization Characteristics of Novel Benzobacteriochlorins Derived from vic-Dihydroxybacteriochlorins (2003)

In a sequence of reactions, methyl mesopyropheophorbide a, mesochlorin e(6) trimethyl ester, mesochlorin p(6) trimethyl ester, mesopurpurin-18-N-hexylimide methyl ester, and mesopurpurin-18-N-3,5-bis(trifluoromethyl)benzylimide methyl ester were synthesized from chlorophyll-a. These chlorins on reacting with osmium tetraoxide produced the corresponding vic-dihydroxybacteriochlorins. The 8-vinylchlorins obtained by refluxing the related vic-dihydroxybacteriochlorins in o-dichlorobenzene were individually treated with dimethylacetylenedicarboxylate (DMAD) under Diels-Alder reaction conditions. The intermediate adducts on 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) treatment rearranged to the corresponding stable benzobacteriochlorins, exhibiting the longest wavelength absorption in the range of 737 to 805 nm. In preliminary in vitro (RIF tumor cells) and in vivo screening (C3H/HeJ mice bearing RIF tumors), some of these compounds were found to be quite effective. Under similar treatment conditions (drug dose: 5.0 micromol/kg; light dose: 135 J/cm(2), tumors were exposed to light for 30 min at 24 h postinjection), the benzobacteriochlorins containing N-substituted-imide ring system produced enhanced photosensitizing efficacy with limited skin phototoxicity. These compounds were also found to bind to site II of human serum albumin (HSA). However, no correlation between the binding constant values and photosensitizing efficacy was observed. A competitive intracellular localization study of these novel structures with Rhodamine-123 (a mitochondrial probe) indicated their preferential localization in mitochondria, without producing any specific displacement of (3)H-PK11195 (PBR probe, (3)H-labeled 1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3-isoquinoline carboxamide). These results suggest that the mitochondrial peripheral benzodiazepine receptor (PBR) is not the cellular binding site for this class of compounds.
Optical Switch Utilizing Total Reflection of (Pb, La)(Zr, Ti)O3 Ceramics (1994)

We have previously proposed an optical deflector which consists of a (Pb, La)(Zr, Ti)O 3 (PLZT) rectangular solid and triangular electrodes on its top and bottom surfaces. Applying DC voltage to both electrodes, the refractive index of the material covered by the electrodes increases and becomes different from that of the remaining part of the plate. Therefore, the part covered by the electrodes works as a prism. By increasing the applied voltage, total reflection takes place at the boundary between the part covered by electrodes and the remainder of the plate. We propose here an optical switch utilizing this total reflection, the electrodes of which are divided into three parts. By applying voltage to two of the three electrodes, the shape of the equivalent prism can be changed. Thus, the output optical beam can be deflected and switched either to the left or right. Since the beam travels straight without voltage application, it is found that this device works as a 1×3 optical switch.
Effects of different concentrations and volumes of formalin on pain response in rats. (2000)

The 10%--25 microL formalin produces fewer flinching responses than other concentrations. Flinching was a biphasic behavior which was more spontaneous and active than was licking. The volume of formalin was a more important factor than the concentration of formalin in the generation of the active biphasic flinching response in the rat model.
THE COMPLEXITY OF THE INFLAMMATORY RESPONSE TO MENINGOCOCCAL SEPSIS REVEALED BY GENE EXPRESSION PROFILING USING CDNA MICROARRAYS (2002)

SOCIETY OF CRITICAL CARE MEDICINE 32ND CRITICAL CARE CONGRESS SAN ANTONIO, TEXAS, USA JANUARY 28-FEBRUARY 2, 2003: ORAL/SANDWICH PRESENTATIONS: Poster Presentation: Basic Science: Sepsis Genetic Markers: PDF Only
Ketonization of Carboxylic Acids by Decarboxylation: Mechanism and Scope (2005)

Abstract In the ketonic decarboxylation process, a ketone is formed from two moles of carboxylic acid; water and carbon dioxide are produced as side‐products. At present, the mechanism of this reaction remains under debate; it has been proposed as a radical mechanism, a mechanism involving a ‐keto acid as intermediate, or a concerted mechanism. This paper demonstrates that the latter mechanism is the most likely one and that weak bases may play the role of promoters. Different processes are reviewed for the syntheses of the following ketones: symmetrical ketones, such as acetone or 3‐pentanone, cyclic ketones, such as cyclopentanone (parent compound and substituted derivatives), fatty ketones, and some unsymmetrical ketones. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2005)
9 kV 4H-SiC IGBTs with 88 mΩ·cm<sup>2</sup> of R <sub>diff, on</sub> (2007)

SiC IGBTs are suitable for high power, high temperature applications. For the first time, the design and fabrication of 9 kV planar p-IGBTs on 4H-SiC are reported in this paper. A differential on-resistance of ~ 88 m(cm2 at a gate bias of –20 V is achieved at 25°C, and decreases to ~24.8 m(cm2 at 200°C. The device exhibits a blocking voltage of 9 kV with a leakage current density of 0.1 mA/cm2. The hole channel mobility is 6.5 cm2/V-s at room temperature with a threshold voltage of –6.5 V resulting in enhanced conduction capability. Inductive switching tests have shown that IGBTs feature fast switching capability at both room and elevated temperatures.
Characterization of CKβ8 and CKβ8-1: Two Alternatively Spliced Forms of Human β-Chemokine, Chemoattractants for Neutrophils, Monocytes, and Lymphocytes, and Potent Agonists at CC Chemokine Receptor 1 (1998)

Two new members of human beta-chemokine cDNA were isolated based on structural and functional similarities to human leukotactin-1. One of these clones was identical to the previously isolated human beta-chemokine, CKbeta8, whereas the other is a splicing variant of CKbeta8, therefore named CKbeta8-1. CKbeta8 was short in 51 nucleotides (17 amino acids) compared with CKbeta8-1. The mature proteins of CKbeta8-1 and CKbeta8 consisted of 116 and 99 amino acids with calculated molecular weights of 12,500 and 10,950, respectively. Both CKbeta8-1 and CKbeta8 were potent agonists at CCR1. These chemokines chemoattracted neutrophils, monocytes, and lymphocytes. They also significantly suppressed colony formation by human bone marrow, granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. To our knowledge, this is the first example that an alternative splicing produces two active beta-chemokines from a single gene.
Laboratory assessment of the status of Her-2/neu protein and oncogene in breast cancer specimens: comparison of immunohistochemistry assay with fluorescence in situ hybridisation assays (2000)

Because of the increasing importance of the Her-2/neu oncogene and oncoprotein in the clinical management of patients with breast cancer, the accurate and consistent evaluation of Her-2/neu status is crucial. This study suggests that the best approach is to combine both IHC and FISH assays; that is, to use the IHC assay as a triage step, followed by the PathVysion FISH assay to analyse the IHC medium and high positive cases.
Alterations of cell surface carbohydrates and inhibition of metastatic property of murine melanomas by alpha 1,3 galactosyltransferase gene transfection. (1995)

Three melanomas of C57BL/6 mice (BL6, JB/MS, and JB/RH) share several phenotypic properties. All these cells contain melanoma-specific ecotropic C-type retrovirus that encodes melanoma-associated antigen recognizable by MM2-9B6 mAb. They do not express H-2Kb molecules, and the alpha-galactosyl epitopes (Gal alpha 1-3Gal beta 1-4GlcNAc-R) they fail to react with soybean agglutinin (SBA), peanut agglutinin (PNA), and vicia villosa (VV) lectins. Previously, we found that failure of BL6 melanoma cells to express alpha-galactosyl epitopes is due to down-regulation of alpha 1,3 galactosyltransferase (alpha 1,3GT) gene expression. To evaluate the possible role of alpha-galactosyl cell membrane carbohydrates in regulation of metastatic properties, individual clones isolated from BL6, JB/MS, and JB/RH melanomas were transfected with alpha 1,3GT cDNA. This resulted in appearance of alpha-galactosyl epitopes, as well as of carbohydrates reacting with SBA, PNA, or VV lectins, but did not affect expression of H-2 class I molecules or melanoma-associated antigen. Appearance of SBA, PNA, and VV lectin binding carbohydrates in the alpha 1,3GT gene-transfected melanoma cells is a result of reduction of cell membrane sialylation and unmasking of these carbohydrates. Reduction in cell membrane sialylation in the alpha 1,3GT gene-transfected melanoma cells is probably due to the competition between alpha 1,3GT with alpha 2,3 sialyltransferase or alha 2,6 sialyltransferase for the common acceptor N-acetyllactosamine in the Golgi apparatus. As a result of this competition, cell membranes of alpha 1,3GT gene-transfected melanoma cells became galactosylated and less sialylated. In parallel with alteration of cell membrane carbohydrates, transfection of the alpha 1,3GT gene leads to the loss of metastatic properties of the transfected melanoma cells in the immunocompetent and immunosuppressed C57BL/6 mice. Thus, the use of specific glycosyltransferase cDNA transfection presents direct experimental confirmation of the importance of cell membrane carbohydrates in the regulation of metastatic properties of tumor cells.
Pharmacological characterization of histamine receptors mediating the stimulation of cyclic AMP accumulation in slices from guinea-pig hippocampus. (1978)

A histamine-sensitive adenylate cyclase presenting characteristics very similar to typical H2-receptors has recently been demonstrated in cell-free brain preparations, whereas the stimulation of cyclic AMP accumulation elicited by histamine in cerebral slices has been postulated to involve both H1- and H2-receptors. We have reinvestigated the pharmacological properties of histamine receptors mediating the stimulation of cyclic AMP in guinea-pig hippocampal slices by determining the relative potencies of several agonists and the apparent affinities of antagonists. Metiamide, a H2-blocker, antagonizes in a competitive manner the stimulation elicited by histamine (or 2-thiazolylethylamine, a predominantly H1-agonist) with a pA2 value of 6, corresponding to an interaction with a single population of typical H2-receptors. The relative potencies of two H2-agonists (dimaprit and 4-methylhistamine) are consistent with this view. However other data suggest the involvement of an heterogeneous population of histamine receptors. First, the relative potencies of two H1-agonists (2-thiazolylethylamine and 2-methylhistamine) in hippocampal slices are intermediate between corresponding values on homogeneous populations of H1- and H2-receptors, respectively. Furthermore in the presence of moderate concentrations (5 nM to 0.1 µM) of mepyramine, an H1-antagonist, the response to histamine is modified in a complex fashion: the responses to histamine in low concentrations remain unaltered whereas those to the amine in concentrations above 10 µM are competitively antagonized. Analysis of these data by Schild plot or by a computer program reveals two components in the response to histamine: a first one (insensitive to mepyramine) with an EC50 of 6µM and a second one with an EC50 of 14 µM for the amine (antagonized by mepyramine with a pA2 of 8.2). The participation of each component to the maximal response is approximately 50%. In addition, a series of H1-antagonists inhibit the response to 100 µM histamine with apparent KB 5 toward the second component which appear to be in the same range as those toward typical H1-receptors. Furthermore the supramaximal response to dimaprit, a pure H2-agonist, is progressively elevated in the presence of 2-thiazolylethylamine, a predominantly H1-agonist, in increasing concentrations. In these conditions the relative potency of 2-thiazolylethylamine becomes comparable with the value for typical H1-receptors. This additive response is blocked by 0.1 µM mepyramine while the response to dimaprit alone is not affected. It is concluded that the response to histamine in brain slices involves the stimulation of both typical H2-receptors and receptors closely similar to (or identical with) H1-receptors, which appear to be activated in a sequential manner.
[Effect of apigenin on protein expressions of PPARs in liver tissues of rats with nonalcoholic steatohepatitis]. (2015)

Apigenin can improve glucose tolerance,lipid metabolism and insulin resistance while decreasing blood levels of TC,TG,LDL-C,FBG,FINS and HOMA-IR,and increasing HDL-C in NASH,as shown in a high-fat diet induced rat model, and may have therapeutic potential.
TSG-6: a multifunctional protein associated with inflammation (2003)

TSG-6 expression is upregulated in many cell types in response to a variety of proinflammatory mediators and growth factors. This protein is detected in several inflammatory disease states (e.g. rheumatoid arthritis) and in the context of inflammation-like processes, such as ovulation, and is often associated with extracellular matrix remodelling. TSG-6 has anti-inflammatory and chondroprotective effects in various models of inflammation and arthritis, which suggest that it is a component of a negative feedback loop capable of downregulating the inflammatory response. Growing evidence also indicates that TSG-6 acts as a crucial factor in ovulation by influencing the expansion of the hyaluronan-rich cumulus extracellular matrix in the preovulatory follicle. TSG-6 is a member of the Link module superfamily and binds to hyaluronan (a vital component of extracellular matrix), as well as other glycosaminoglycans, via its Link module. In addition, TSG-6 forms both covalent and non-covalent complexes with inter-alpha-inhibitor (a serine protease inhibitor present at high levels in serum) and potentiates its anti-plasmin activity.
Laplacian Mesh Processing (2005)

Surface representation and processing is one of the key topics in computer graphics and geometric modeling, since it greatly affects the range of possible applications. In this paper we will present recent advances in geometry processing that are related to the Laplacian processing framework. This framework is based on linear operators defined on polygonal meshes, and furnishes a variety of processing applications, such as shape approximation and compact representation, mesh editing, filtering, watermarking and morphing. The core of the framework is the mesh Laplacian operator, which allows to define differential coordinates and new bases for efficient mesh geometry representation.
‘Well-determined’ regions in RNA secondary structure prediction: analysis of small subunit ribosomal RNA (1995)

Recent structural analyses of genomic RNAs from RNA coliphages suggest that both well-determined base paired helices and well-determined structural domains that are identified by "energy dot plot" analysis using the RNA folding package mfold, are likely to be predicted correctly. To test these observations with another group of large RNAs, we have analyzed 15 ribosomal RNAs. Published secondary structure models that were derived by comparative sequence analysis were used to evaluate the predicted structures. Both the optimal predicted fold and the predicted "energy dot plot" of each sequence were examined. Each prediction was obtained from a single computer run on an entire ribosomal RNA sequence. All predicted base pairs in optimal foldings were examined for agreement with proven base pairs in the comparative models. Our analyses show that the overall correspondence between the predicted and comparative models varied for different RNAs and ranges from a low of 27% to high of 70%, with a mean value of 49%. The correspondence improves to a mean value of 81% when the analysis is limited to well-determined helices. In addition to well-determined helices, large well-determined structural domains can be observed in "energy dot plots" of some 16S ribosomal RNAs. The predicted domains correspond closely with structural domains that are found by the comparative method in the same RNAs. Our analyses also show that measuring the agreement between predicted and comparative secondary structure models underestimates the reliability of structural prediction by mfold.