gebhart/patrepeval huggingface dataset:
Removal of repeated sequences from hybridisation probes (1985)

Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.
Long-term efficacy of the complement inhibitor eculizumab in cold agglutinin disease (2009)

To the editor:

Cold agglutinin disease (CAD) is characterized by immunoglobulin M (IgM)–mediated hemagglutination and robust complement activation leading to intravascular hemolysis and hemolysis-related symptoms including anemia, fatigue, dyspnea, hemoglobinuria, and acrocyanosis.[1][1][⇓][2
New β-Lactamase Inhibitors: a Therapeutic Renaissance in an MDR World (2013)

As the incidence of Gram-negative bacterial infections for which few effective treatments remain increases, so does the contribution of drug-hydrolyzing β-lactamase enzymes to this serious clinical problem. This review highlights recent advances in β-lactamase inhibitors and focuses on agents with novel mechanisms of action against a wide range of enzymes. To this end, we review the β-lactamase inhibitors currently in clinical trials, select agents still in preclinical development, and older therapeutic approaches that are being revisited. Particular emphasis is placed on the activity of compounds at the forefront of the developmental pipeline, including the diazabicyclooctane inhibitors (avibactam and MK-7655) and the boronate RPX7009. With its novel reversible mechanism, avibactam stands to be the first new β-lactamase inhibitor brought into clinical use in the past 2 decades. Our discussion includes the importance of selecting the appropriate partner β-lactam and dosing regimens for these promising agents. This "renaissance" of β-lactamase inhibitors offers new hope in a world plagued by multidrug-resistant (MDR) Gram-negative bacteria.
Bidirectional modulation of anxiety-related and social behaviors by amygdala projections to the medial prefrontal cortex (2015)

The basolateral amygdala (BLA) and the medial prefrontal cortex (mPFC) modulate anxiety and social behaviors. It remains to be elucidated, however, whether direct projections from the BLA to the mPFC play a functional role in these behaviors. We used optogenetic approaches in behaving mice to either activate or inhibit BLA inputs to the mPFC during behavioral assays that assess anxiety-like behavior and social interaction. Channelrhodopsin-2 (ChR2)-mediated activation of BLA inputs to the mPFC produced anxiogenic effects in the elevated plus maze and open field test, whereas halorhodopsin (NpHR)-mediated inhibition produced anxiolytic effects. Furthermore, activation of the BLA-mPFC pathway reduced social interaction in the resident-intruder test, whereas inhibition facilitated social interaction. These results establish a causal relationship between activity in the BLA-mPFC pathway and the bidirectional modulation of anxiety-related and social behaviors.
Targeted treatment of recurrent platinum-resistant ovarian cancer: current and emerging therapies (2010)

With advances in surgical techniques and chemotherapeutic agents, mortality rates from epithelial ovarian cancer (EOC) have slightly decreased over the last 30 years. However, EOC still ranks as the most deadly gynecologic cancer with an overall 5-year survival rate of 45%. Prognosis is especially disappointing for women with platinum-resistant disease, where 80% of patients will fail to respond to available therapies. Emerging treatment strategies have sub-sequently focused on targets which are integral to tumor growth and metastasis. In this review, we will focus on those innovative agents currently under investigation in clinical trials.
Implementation of the 2D DCT using a Xilinx XC6264 FPGA (2002)

This paper presents a novel FPGA implementation of a two dimensional (8/spl times/8) point Discrete Cosine Transform. It is shown how the development of a suitable architectural style can produce high quality circuit designs for a specific technology, in this case the Xilinx XC6200 series of FPGA. Distributed arithmetic and exploitation of parallelism and pipelining are used to produce a DCT implementation on a single FPGA that operates at 25 frames per second with VGA resolution which is the equivalent of 2 million multiplications or additions per second.
A lexicon driven approach to handwritten word recognition for real-time applications (1997)

A fast method of handwritten word recognition suitable for real time applications is presented in this paper. Preprocessing, segmentation and feature extraction are implemented using a chain code representation of the word contour. Dynamic matching between characters of a lexicon entry and segment(s) of the input word image is used to rank the lexicon entries in order of best match. Variable duration for each character is defined and used during the matching. Experimental results prove that our approach using the variable duration outperforms the method using fixed duration in terms of both accuracy and speed. Speed of the entire recognition process is about 200 msec on a single SPARC-10 platform and the recognition accuracy is 96.8 percent are achieved for lexicon size of 10, on a database of postal words captured at 212 dpi.
Sensitivity to Basis Mismatch in Compressed Sensing (2011)

The theory of compressed sensing suggests that successful inversion of an image of the physical world (broadly defined to include speech signals, radar/sonar returns, vibration records, sensor array snapshot vectors, 2-D images, and so on) for its source modes and amplitudes can be achieved at measurement dimensions far lower than what might be expected from the classical theories of spectrum or modal analysis, provided that the image is sparse in an apriori known basis. For imaging problems in spectrum analysis, and passive and active radar/sonar, this basis is usually taken to be a DFT basis. However, in reality no physical field is sparse in the DFT basis or in any apriori known basis. No matter how finely we grid the parameter space the sources may not lie in the center of the grid cells and consequently there is mismatch between the assumed and the actual bases for sparsity. In this paper, we study the sensitivity of compressed sensing to mismatch between the assumed and the actual sparsity bases. We start by analyzing the effect of basis mismatch on the best <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</i> -term approximation error, which is central to providing exact sparse recovery guarantees. We establish achievable bounds for the <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">l</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> error of the best <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</i> -term approximation and show that these bounds grow linearly with the image (or grid) dimension and the mismatch level between the assumed and actual bases for sparsity. We then derive bounds, with similar growth behavior, for the basis pursuit <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">l</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> recovery error, indicating that the sparse recovery may suffer large errors in the presence of basis mismatch. Although, we present our results in the context of basis pursuit, our analysis applies to any sparse recovery principle that relies on the accuracy of best <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</i> -term approximations for its performance guarantees. We particularly highlight the problematic nature of basis mismatch in Fourier imaging, where spillage from off-grid DFT components turns a sparse representation into an incompressible one. We substantiate our mathematical analysis by numerical examples that demonstrate a considerable performance degradation for image inversion from compressed sensing measurements in the presence of basis mismatch, for problem sizes common to radar and sonar.
Effects of Perioperative Granulocyte Colony‐Stimulating Factor on Horses With Ascending Colonic Ischemia (1993)

Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein that regulates the proliferation and maturation of hematopoietic progenitor cells and modulates the function of mature neutrophils. The responses to administration of G-CSF alone, and in combination with antimicrobials, were studied in an equine model of ascending colon ischemia. Complete segmental colonic ischemia (3.75 hours) with pelvic flexure enterotomy was created in four treatment groups. Group 1 horses received recombinant canine G-CSF (10 micrograms/kg, every 24 hours, intramuscularly), gentamicin sulfate (2.2 mg/kg, every 8 hours, intravenously), and potassium penicillin G (40,000 IU/kg, every 6 hours, intravenously). Group 2 horses were treated with the G-CSF vehicle and antimicrobials as for group 1. Group 3 horses received G-CSF and the antimicrobial drug vehicles, and group 4 horses served as the untreated control receiving G-CSF vehicle and antimicrobial vehicles. The results for 20 horses, five horses in each group, were compared. Treatment with G-CSF was associated with an increased concentration of white blood cells, band neutrophils, neutrophils, lymphocytes, and monocytes in the peripheral blood after surgery. Antimicrobial administration had no detectable effect on cell concentrations after surgery. Administration of G-CSF was associated with an increased concentration of nucleated cells in the peritoneal fluid including neutrophils, small mononuclear cells and large mononuclear cells. Horses that developed incisional infections had lower neutrophil concentrations in the peripheral blood on postoperative day 2 than horses without infected incisions. These results suggested that the prophylactic administration of G-CSF may be useful in the treatment of patients at risk for developing neutropenia after surgery.
Newcastle disease virus-infected splenocytes express the proopiomelanocortin gene. (1986)

Infection of murine splenocytes with Newcastle disease virus results in expression of the proopiomelanocortin gene as determined by dot and northern blot analysis of total cellular and poly(A)+ cytoplasmic RNA. These data provide the first evidence that the precursor protein for adrenocorticotropin and beta-endorphin can be synthesized by lymphoid cells.
A Short Visit to the Bot Zoo (2005)

The past year (2004-5) has seen, a new attack trend emerge: bots. After a successful compromise, the attacker installs a bot (also called a zombie or drone) on the system; this small program enables a remote control mechanism to then command the victim. Attackers use this technique repeatedly to form networks of compromised machines (botnets) to further enhance the effectiveness of their attacks. In recent years, malicious bots have become commonplace, with botnets in particular posing a severe threat to the Internet community. Attackers primarily use them for distributed denial-of-service (DDoS) attacks, mass identity theft, or sending spam.
Ionizing radiation improves survival in mice bearing intracranial high-grade gliomas injected with genetically modified herpes simplex virus. (1999)

Malignant gliomas remain incurable with current interventions. Encouraging investigational approaches include the use of genetically modified herpes simplex-1 (HSV-1) viruses as direct cytotoxic agents. Combining attenuated HSV-1 with standard therapy, human U-87 malignant glioma xenografts grown in the hind limb or intracranially in athymic nude mice were exposed to ionizing radiation, inoculated with genetically modified HSV R3616, or received both virus and radiation. The combination of virus with fractionated ionizing radiation suggests a synergistic action and results in reduced tumor volumes and longer survivals when compared with treatment with either modality alone.
Resistance of Akt kinases to dephosphorylation through ATP-dependent conformational plasticity (2011)

Phosphorylation of a threonine residue (T308 in Akt1) in the activation loop of Akt kinases is a prerequisite for deregulated Akt activity frequently observed in neoplasia. Akt phosphorylation in vivo is balanced by the opposite activities of kinases and phosphatases. Here we describe that targeting Akt kinase to the cell membrane markedly reduced sensitivity of phosphorylated Akt to dephosphorylation by protein phosphatase 2A. This effect was amplified by occupancy of the ATP binding pocket by either ATP or ATP-competitive inhibitors. Mutational analysis revealed that R273 in Akt1 and the corresponding R274 in Akt2 are essential for shielding T308 in the activation loop against dephosphorylation. Thus, occupancy of the nucleotide binding pocket of Akt kinases enables intramolecular interactions that restrict phosphatase access and sustain Akt phosphorylation. This mechanism provides an explanation for the "paradoxical" Akt hyperphosphorylation induced by ATP-competitive inhibitor, A-443654. The lack of phosphatase resistance further contributes insight into the mechanism by which the human Akt2 R274H missense mutation may cause autosomal-dominant diabetes mellitus.
Graft-versus-Host Disease and Survival in Patients with Aplastic Anemia Treated by Marrow Grafts from HLA-Identical Siblings (1983)

One hundred thirty patients with severe aplastic anemia were conditioned with cyclophosphamide for transplantation of marrow from HLA-identical siblings. The patients were selected for the present analysis according to the criterion of sustained marrow engraftment. Of the 130 patients, 97 are now alive between 1.4 and 11 years (median, 5) after transplantation. Twenty-nine of the thirty-three who died had either acute or chronic graft-versus-host disease (GVHD). Our analysis was directed at identifying factors predicting GVHD and survival after transplantation in patients. Our key findings were that moderately severe to severe acute GVHD had a strong adverse influence on survival; that a protective environment significantly reduced mortality, which corresponded in part to a reduction in and delayed onset of acute GVHD; that refractoriness to random-donor platelet infusions at transplantation adversely influenced survival, particularly among patients with acute GVHD; and that increasing age was associated with increased mortality.
Mononuclear heterocyclic rearrangements—vi : Conversion of 1,2,4-oxadiazoles into imidazoles (1974)

Abstract The rearrangement of N - (1,2,4 - oxadiazol - 3 - yl)β - enamino ketones 3 a–d and N-(1,2,4 - oxadiazol - 3 - yl)β - enaminoesters 3 e–h into 2 - acylamino - imidazolyl derivatives 9 a–h by the action of sodium ethoxide in N,N-dimethylformamide is the first example of a mononuclear heterocyclic rearrangement involving a nucleophilic carbon. Compounds prepared by condensation of 3 - amino - 1,2,4 - oxadiazoles 1 a–b with β - dicarbonyl compounds 2 a–d, show spectroscopic properties in agreement with cis-chelated structures 4. By condensation between 1 a–b and benzoylacetic ester 2 d, N - (1,2,4 - oxadiazol - 3 - yl)β - ketoamides 6 a–b have been obtained as secondary products. In solution, these compounds are in equilibrium with the corresponding tautomers 7 a–b.
The Potential Benefit of Radiotherapy with Protons in Head and Neck Cancer with Respect to Normal Tissue Sparing: A Systematic Review of Literature (2011)

The results of ISPC studies indicate that protons have the potential for a significantly lower normal tissue dose, while keeping similar or better target coverage. Scanned IMPT probably offers the most advantage and will allow for a substantially lower probability of radiation-induced side effects. The results of these ISPC studies should be confirmed in properly designed clinical trials.
Expression of CCR2 by Endothelial Cells (1999)

Endothelial cell proliferation and migration may play a central role in angiogenesis, wound healing, and atherosclerosis. Although CXC chemokines can act on endothelial cells by influencing proliferation, an involvement of CC chemokines and endothelial expression of chemokine receptors remains to be elucidated. Reverse transcription-polymerase chain reaction, RNase protection, Western blot, and flow cytometric analysis showed that human umbilical vein endothelial cells express mRNA and surface protein of the monocyte chemotactic protein-1 (MCP-1) receptor CCR2, which was upregulated by inflammatory cytokines. MCP-1 induced migration of endothelial cells in a transwell assay, which was inhibited by the 9-76 MCP-1 receptor antagonist. Increased secretion of MCP-1 or interleukin-8, but not RANTES, on endothelial injury suggested a functional role of CCR2 in wound repair as measured by ELISA. After mechanical injury to endothelial monolayers, which spontaneously closed within 24 hours, wound repair was delayed by the 9-76 antagonist and by a blocking monoclonal antibody to MCP-1, but not to interleukin-8, and was improved by exogenous MCP-1. This was confirmed by quantification of cell migration into the wound area, whereas proliferation and viability were unaltered by MCP-1 or its analogue. Notably, immunohistochemistry of inflamed tissue revealed CCR2 staining on arterial, venous, and venular endothelium affected by cellular infiltration. This is the first demonstration of endothelial CCR2 expression ex vivo, inferring its involvement in inflammatory conditions. Thus endothelial cells express functional CCR2 that may have important implications for endothelial wound repair and inflammatory reactions.
An introduction to structural health monitoring (2006)

The process of implementing a damage identification strategy for aerospace, civil and mechanical engineering infrastructure is referred to as structural health monitoring (SHM). Here, damage is defined as changes to the material and/or geometric properties of these systems, including changes to the boundary conditions and system connectivity, which adversely affect the system's performance. A wide variety of highly effective local non-destructive evaluation tools are available for such monitoring. However, the majority of SHM research conducted over the last 30 years has attempted to identify damage in structures on a more global basis. The past 10 years have seen a rapid increase in the amount of research related to SHM as quantified by the significant escalation in papers published on this subject. The increased interest in SHM and its associated potential for significant life-safety and economic benefits has motivated the need for this theme issue. This introduction begins with a brief history of SHM technology development. Recent research has begun to recognize that the SHM problem is fundamentally one of the statistical pattern recognition (SPR) and a paradigm to address such a problem is described in detail herein as it forms the basis for organization of this theme issue. In the process of providing the historical overview and summarizing the SPR paradigm, the subsequent articles in this theme issue are cited in an effort to show how they fit into this overview of SHM. In conclusion, technical challenges that must be addressed if SHM is to gain wider application are discussed in a general manner.
Molecular and clinical pharmacology of intranasal corticosteroids: clinical and therapeutic implications (2008)

Intranasal corticosteroids (INSs) are effective treatments for allergic rhinitis, rhinosinusitis, and nasal polyposis. In recent years, increased understanding of corticosteroid and glucocorticoid receptor pharmacology has enabled the development of molecules designed specifically to achieve potent, localized activity with minimal risk of systemic exposure. Pharmacologic potency studies using affinity and other assessments have produced similar rank orders of potency, with the most potent being mometasone furoate, fluticasone propionate, and its modification, fluticasone furoate. The furoate and propionate ester side chains render these agents highly lipophilic, which may facilitate their absorption through nasal mucosa and uptake across phospholipid cell membranes. These compounds demonstrate negligible systemic absorption. Systemic absorption rates are higher among the older corticosteroids (flunisolide, beclomethasone dipropionate, triamcinolone acetonide, and budesonide), which have bioavailabilities in the range of 34-49%. Studies, including 1-year studies with mometasone furoate, fluticasone propionate, and budesonide that evaluated potential systemic effects of INSs in children have generally found no adverse effects on hypothalamic-pituitary-adrenal axis function or growth. Clinical data suggest no significant differences in efficacy between the INSs. Theoretically, newer agents with lower systemic availability may be preferable, and may come closer to the pharmacokinetic/pharmacologic criteria for the ideal therapeutic choice.
The synthesis of [18F]fluoroarenes from the reaction of cyclotron-produced [18F]fluoride ion with diaryliodonium salts (1998)

Diaryliodonium salts have been shown to react with fluoride ion at 80 °C in acetonitrile to generate aryl fluorides. The regioselectivity is controlled electronically and by the bulk of the ortho-substituents on the rings, with the latter the dominant factor such that electron-rich rings can be fluorinated. ortho-Substituted aryl fluorides can be selectively produced from unsymmetrical diaryliodonium salts. The process has been used to synthesise [18F] labelled aromatics by the use of cyclotron generated [18F]fluoride ion.
Problems in Hemostasis during Open Heart Surgery (1967)

Gans, Henry M.D.Ph.D.; Castaneda, Aldo R. M.D., Ph.D.; Subramanian, V. M.D.; John, Stanley M.D.; Lhliehei, C. Walton M.D.,Ph.D. Author Information
Das Nebeneinander der beiden deutschen Staaten und die deutsche Einigung 1990 (1999)

ZusammenfassungMit dem Fall der Berliner Mauer in der Nacht vom 9. November 1989 begann eine rasante Entwicklung, die den Beobachter noch heute in Staunen versetzt. Innerhalb eines Zeitraumes von etwas mehr als einem Jahr verschwand der Staat DDR von der politischen Landkarte. Mit dem Beitritt der DDR zur Bundesrepublik Deutschland am 3. Oktober 1990 wurde die staatliche Einheit Deutschlands Wirklichkeit. Noch im selben Jahr fanden die erste gesamtdeutsche Bundestagswahl und die Konstitution des ersten gesamtdeutschen Bundestages statt.