gebhart/patrepeval huggingface dataset:
<title>Current status of DNA sequencing by single molecule detection</title> (1999)

Our current experiments further the development of a laser- based technique capable of sequencing an individual strand of DNA. We report the detection and identification of fluorescently labeled nucleotides enzymatically cleaved from DNA strands suspended in flow. We used fluorescence lifetime, fluorescence intensity, or a correlated measure of the intensity and lifetime to identify each individual tagged base traversing the detection region with high accuracy. DNA strands containing a single tetramethylrhodamine labeled uracil and/or a single Rhodamine 6G labeled cytosine were attached to polystyrene microspheres. An optical trap was used to capture and hold a single DNA-laden microsphere nominally 20 microns upstream of the detection region of an ultra- sensitive flow cytometer. The addition of an exonuclease cleaved bases from the 3' end of the fluorescently labeled strand. The cleaved, labeled nucleotides were carried by the flow downstream and detected and identified one-at-a-time with high efficiency by laser-induced fluorescence.
News Briefs (2007)

When many people think of motion-capture technology, they think of people wearing reflective markers and having their movements captured on camera for use in modeling figures created by animation or computer graphics. Motion capture is frequently associated with movies or videogames. Now, motion technology is moving in some new directions. Researchers are working on techniques that don't, for example, require placing hundreds of markers on models. Also, motion-capture applications are becoming less expensive and more readily available. Fields as diverse as medicine, manufacturing, and advertising are thus beginning to utilize the technology more often. Nonetheless, motion capture still faces challenges, such as output precision and ease of use.
Stereospecific total syntheses of dl-coriolin and dl-coriolin B (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereospecific total syntheses of dl-coriolin and dl-coriolin BSamuel Danishefsky, Robert Zamboni, Michael Kahn, and Sarah Jane EtheredgeCite this: J. Am. Chem. Soc. 1981, 103, 12, 3460–3467Publication Date (Print):June 1, 1981Publication History Published online1 May 2002Published inissue 1 June 1981https://pubs.acs.org/doi/10.1021/ja00402a034https://doi.org/10.1021/ja00402a034research-articleACS PublicationsRequest reuse permissionsArticle Views976Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Dichlorotetrakis(dimethyl sulphoxide)ruthenium(II) and its use as a source material for some new ruthenium(II) complexes (1973)

A simple preparation of dichlorotetrakis(dimethyl sulphoxide)ruthenium(II) is given. The i.r. and 1H n.m.r. spectra of the complex suggest there are mixed sulphur and oxygen co-ordination sites. The complex is a useful starting material for other ruthenium(II) complexes.
Mechanisms of Ultrasonic Modulation of Multiply Scattered Coherent Light: An Analytic Model (2001)

An analytic model of the ultrasonic modulation of multiply scattered coherent light in scattering media is developed based on two mechanisms: the ultrasonic modulation of the index of refraction and the ultrasonic modulation of the displacements of Rayleigh scatterers. In water solutions, for example, the first mechanism is slightly less important than the second mechanism when the scattering mean free path is less than a critical fraction (0.0890) of the acoustic wavelength, and it becomes increasingly more important beyond this point. This model agrees well with an independent Monte Carlo model.
The Structure and Emission of the Accretion Shock in T Tauri Stars (1998)

We have examined one of the key predictions of the magnetospheric infall model for classical T Tauri stars (CTTS), namely, the formation of a shock on the stellar surface. We nd that accretion column emission can successfully reproduce the main observational properties of the excess continuum that veils the absorption features in CTTS. This success adds further support to the magnetospheric infall model for disk accretion. We have calculated the structure and the spectral energy distribution of the emergent continuum emission from a shock at the base of the magnetospheric accretion column. We nd that the spectral shape of the excess can be understood as optically thick emission from the heated photosphere below the shock, appearing mostly on the Paschen and Brackett continua, and optically thin emission from the preshock and attenuated postshock regions, dominating at wavelengths shorter than the Balmer threshold. The accretion luminosity and rate depend on two parameters : the energy ux of the accretion ow, F, and the surface coverage of the column, f, with typical values log F D 10.511.5, of the order or up to a factor of 10 higher than the intrinsic stellar ux, and f D 0.1%1% of the surface area. The so-called continuum stars have accretion columns with similar energy uxes than the less veiled, typical CTTS but with much larger surface emitting areas of f 10%. At near-infrared wavelengths, the predicted veiling from the accretion column for typical CTTS parameters is nearly constant and 0.1. Only for the "" continuum stars are signicant amounts of near-infrared veiling from the accretion column expected. For accretion columns with low values of F, the Paschen continuum emission has spectral features, rendering the usual deveiling procedures uncertain. Magnetospheric ow Balmer emission may also contribute to the excess for stars with low-F columns. Finally, accretion column emission can successfully reproduce the observed correlation between the excess luminosity at the U band and the accretion luminosity. This correlation does not depend on the characteristics (mass, spectral type) of the underlying star, at least in the range M3K5.
Evaluation of a Multiplex Fluorescent Microsphere Immunoassay for the Determination of Epstein-Barr Virus Serologic Status (2007)

Epstein-Barr virus (EBV), a human herpesvirus, affects up to 95% of adults. Diagnosis of acute EBV infection can be challenging and often relies on the serologic antibody pattern to 3 distinct antigens, most often determined by indirect fluorescent antibody (IFA), enzyme-linked immunosorbent assays (ELISAs), and, more recently, multiplex assays. We compared a multiplex assay for the simultaneous detection of antibodies to viral capsid (VCA), nuclear (EBNA), and early (EA) EBV antigens with ELISAs using IFA for discrepancy resolution. Concordance of the multiplex assay was good for all 4 antigens: VCA IgM, 86.6% vs ELISA and 92.9% vs IFA; VCA IgG, 92.8% vs ELISA and 98.0% vs IFA; EBNA IgG, 90.3% vs ELISA and 98.1% vs IFA; and EA IgG, 83.8% vs ELISA and 92.8% vs IFA. After IFA resolution, correlation between the multiplex assay and ELISA for serologic disease stage, based on the antibody profile of all 4 analytes, was 90%. The multiplex assay showed good correlation with an established ELISA and even better correlation with the "gold standard" IFA. Advantages of the multiplex assay over traditional methods include multiple results per assay, inclusion of internal controls for each assay, and well-to-well monitoring of assay drift.
Oxidation-reduction properties and complexation reactions of the iron-molybdenum cofactor of nitrogenase. (1980)

The interactions of the iron-molybdenum cofactor, FeMoco, isolated from acid-treated Azotobacter vinelandii molybdenum-iron protein (Av1) with EDTA and thiophenol in N-methylformamide solution have been reinvestigated. Our studies show that EDTA alone is sufficient to eliminate the EPR signal of dithionite-reduced FeMoco. Neither light/5-deazaflavin nor carbon monoxide are required, which implies that this EPR-silent form of FeMoco does not correspond to the EPR-silent, substrate-reducing state of Av1. As EDTA-treated FeMoco does not regain EPR activity on addition of sodium dithionite or thiophenol, it is apparently distinct from the EPR-silent form of either dye-oxidized FeMoco or dye-oxidized Av1. Thiophenol sharpens the EPR signal of dithionite-reduced FeMoco and shifts the g = 3.3 feature to g = 3.6. This shift is complete at 1:1 ratio of thiophenol/Mo atom, while the EDTA effect requires about 40 molecules/Mo atom. Thiophenol and EDTA probably affect different sites of FeMoco. The binding of either reactant does not affect the activity of FeMoco as measured by its ability to reconstitute extracts of A. vinelandii mutant UW45.
A Survey of Electrochemical Super-Capacitor Technology (2003)

The electrochemical double-layer capacitor (EDLC) is an emerging technology that promises to play an important role in meeting the demands of electronic devices and systems both now and in the future.This paper traces the history of the development of the technology.and explores the principles and theory of operation.The use of EDLCs in applications such as pulse power, backup sources, and others is discussed and comparisons made with alternative technologies.To provide an example with which to outline practical implementation issues, a UPS incorporating EDLCs for energy storage is also explored.
Efficacy and safety of an interleukin 6 monoclonal antibody for the treatment of systemic lupus erythematosus: a phase II dose-ranging randomised controlled trial (2016)

NCT01405196; Pre-results.
ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase. (1994)

Two recessive alleles of ERG10 and three temperature-sensitive recessive alleles of HMG1 (3-hydroxy-3-methyl-glutaryl-CoA reductase isoenzyme 1) were isolated in a screen for mevalonate auxotrophs in Saccharomyces cerevisiae. The essential, single-copy ERG10 gene was cloned by complementation of the temperature-sensitive phenotype of erg10-21. The 1,194-base pair continuous open reading frame, encoding a 398-amino acid polypeptide with a calculated molecular mass of 41,681 daltons, was demonstrated to encode cytoplasmic aceto-acetyl-CoA thiolase. Acetoacetyl-CoA thiolase activity corresponded to the number of copies of ERG10 present in cell extracts, and null alleles of ERG10 produced no detectable acetoacetyl-CoA thiolase enzyme activity. The deduced amino acid sequence was 40-95% identical to acetoacetyl-CoA thiolases from other organisms. This identity included the active site cysteines located at amino acids 91 and 384 in the Erg10 protein.
Electrofusion‐induced intracellular Ca2+ flux and its effect on murine oocyte activation (1992)

These experiments were designed to monitor influx of extracellular Ca2+ into the murine ooplasm following a 1.56 kV.cm-1 direct current (DC) electrofusion pulse and subsequently to determine its effect on rate of activation. Pulse media consisted of non-electrolyte (0.3 M mannitol) and electrolyte (phosphate-buffered saline; PBS) media each containing 0.0, 0.05, or 0.9 mM Ca2+ (groups T1-T3 and T4-T6, respectively). Cumulus-free oocytes were incubated in 100 microliters drops of PBS containing 2 microM of the calcium indicator fluo-3/AM for 60 min at 37 degrees C. Fluo-3/AM-loaded oocytes were equilibrated for 7 min in assigned treatment media (T1-T6) prior to application of DC pulse. Change in fluorescent intensity was monitored for 6.5 min after DC pulse by photon counting spectrofluorometry. Fluorometric measurements demonstrate a dramatic rise in intracellular free Ca2+ (Ca2+i) following DC pulse is associated with Ca2+ ion concentration in the pulse medium. Significantly (P less than 0.01) higher Ca2+i levels were observed when 0.9 mM Ca2+ was added to the pulse medium (T3 and T6) compared with pulse medium containing lower Ca2+ ion concentrations (T1, T2, T4, and T5; P greater than 0.05). Differences (P less than 0.01) were observed in peak Ca2+i levels 18 sec after pulse with mean percent change in fluorescence of 5.1%a, 33.9%b, 112.7%c, 1.2%a, 9.3%a, and 99.9%c for T1-T6, respectively (values with different superscripts are significantly different at P less than 0.01). Increased oocyte membrane permeability to Ca2+ ion after DC pulse was observed for a minimum of 5 min after delivery of the 1.56 kV.cm-1 pulse.(ABSTRACT TRUNCATED AT 250 WORDS)
Incidence of adverse events attributable to bupivacaine liposome injectable suspension or plain bupivacaine for postoperative pain in pediatric surgical patients: A retrospective matched cohort analysis (2018)

In a cohort of pediatric surgical patients receiving wound infiltration with either plain or liposomal bupivacaine, we identified no cases of local anesthetic systemic toxicity syndrome, and only few patients with any sign or symptom that could potentially be related to local anesthetic toxicity.
Helix-Loop-Helix Proteins: Regulators of Transcription in Eucaryotic Organisms (2000)

Click to increase image sizeClick to decrease image size ACKNOWLEDGMENTSWe thank the reviewers for their helpful comments and suggestions which improved the quality of the manuscript.M.E.M. is supported by a postdoctoral Cancer Biology training grant from the National Institutes of Health. C.M. is supported by grants from the National Institutes of Health.
Ab initio molecular dynamics study of the hydration of Li+, Na+ and K+ in a montmorillonite model. Influence of isomorphic substitution (2009)

The present work reports ab initio molecular dynamics simulations, based on density functional theory using the PBE functional, of Li+- Na+- and K+-montmorillonites, considering three types of isomorphic substitutions in the montmorillonite layer: tetrahedral (Tsub), octahedral (Osub) and both (OTsub). These simulations allow us to evaluate the effect of each type of substitution on the inner- outer-sphere complex formation of the alkali cations. It is observed that, for the three kinds of substituted montmorillonites, K+ remains bound to the surface confirming its role as swelling inhibitor. In contrast, Li+ tends to hydrate and coordinate to 4 water molecules in all cases, except for OTsub, for which one of the two Li+ cations remains bound to the oxygens close to the substituted tetrahedral site. Finally, Na+ presents an intermediate behaviour, binding to the surface in Tsub montmorillonite but being hydrated in Osub. These simulations show that the hydration/adsorption behaviour of alkali cations in the swelling process of montmorillonite depends on the affinity of the cation for water and the surface, as well as on the type of substitution that controls the negative charge on surface oxygen atoms.
A Nuclear Localization Sequence Endows Human Pancreatic Ribonuclease with Cytotoxic Activity (2004)

Some members of the ribonuclease superfamily, such as Onconase, are cytotoxic to cancer cells. This is not the case for human pancreatic ribonuclease. This lack of cytotoxicity is probably a result of the inhibition exerted by the cytosolic ribonuclease inhibitor once the protein has reached the cytosol. Until now, all cytotoxic human pancreatic ribonuclease variants have been described as being resistant to the inhibitor. Here, we report on the characterization of a cytotoxic variant of human pancreatic ribonuclease which has an Arg triplet introduced onto one of its surface-exposed loops. Despite its sensitivity to the inhibitor, this variant, called PE5, was only 5-15 times less cytotoxic than Onconase. When it was taken up by cells, it was only observed within late compartments of the endocytic pathway, probably because the number of molecules transported to the cytosol was too small to allow their visualization. Nuclear import assays showed that the Arg triplet endows PE5 with a nuclear localization signal. In these experiments, PE5 was efficiently transported to the nucleus where it was initially localized in the nucleolus. Although the Arg introduction modified the net charge of the protein and somehow impaired recognition by the cytosolic inhibitor, control variants, which had the same number of charges or were not recognized by the inhibitor, were not toxic. We concluded that targeting a ribonuclease to the nucleus results in cytotoxicity. This effect is probably due to ribonuclease interference with rRNA processing and ribosome assembly within the nucleolus.
Finding image exemplars using fast sparse affinity propagation (2008)

In this paper, we propose a novel approach to organize image search results obtained from state-of-the-art image search engines in order to improve user experience. We aim to discover exemplars from search results and simultaneously group the images. The exemplars are delivered to the user as a summary of search results instead of the large amount of unorganized images. This gives the user a brief overview of search results with a small amount of images, and helps the user to further find the images of interest. We adopt the idea of affinity propagation and design a fast sparse affinity propagation algorithm to find exemplars that best represent the image search results. Experiments on real-world data demonstrate the effectiveness of our method both visually and quantitatively.
Molecular Diagnostics of Human Cancer (1989)

Proceedings of the 1988 Cancer Cells Conference. Major topics covered include: molecular analysis of chromosomal rearrangements in leukemia and lymphoma, chromosomal alterations and drug resistant genes in solid tumors, nuclear oncogene-encoded proteins, activated oncogene proteins, DNA tumor virus
A direct conversion RF front-end for 2-GHz WCDMA and 5.8-GHz WLAN applications (2003)

A direct conversion RF front-end for 2.0 GHz WCDMA and 5.8 GHz WLAN applications is described. The measured double-sideband NF, IIP3 and voltage gain are 3.6 dB, -15.1 dBm and 29.5 dB for WCDMA, and 5.2 dB, -17.4 dBm and 26.5 dB for WLAN, respectively. The RF front-end consumes 22.3 mA in WCDMA mode and 23.1 mA in WLAN mode from a 2.7 V supply. The chip is fabricated using a 0.35 /spl mu/m 45 GHz SiGe BiCMOS process.
Cloning of a Human Receptor of the NPY Receptor Family with High Affinity for Pancreatic Polypeptide and Peptide YY (1995)

Neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP) are structurally related peptides found in all higher vertebrates. NPY is expressed exclusively in neurons, whereas PYY and PP are produced primarily in gut endocrine cells. Several receptor subtypes have been identified pharmacologically, but only the NPY/PYY receptor of subtype Y1 has been cloned. This is a heptahelix receptor that couples to G proteins. We utilized Y1 sequence information from several species to clone a novel human receptor with 43% amino acid sequence identity to human Y1 and 53% identity in the transmembrane regions. The novel receptor displays a pharmacological profile that distinguishes it from all previously described NPY family receptors. It binds PP with an affinity (Ki) of 13.8 pM, PYY with 1.44 nM, and NPY with 9.9 nM. Because these data may identify the receptor as primarily a PP receptor, we have named it PP1. In stably transfected Chinese hamster ovary cells the PP1 receptor inhibits forskolin-stimulated cAMP synthesis. Northern hybridization detected mRNA in colon, small intestine, pancreas, and prostate. As all three peptides are present in the gut through either endocrine release or innervation, all three peptides may be physiological ligands to the novel NPY family receptor PP1.
Post-disaster housing reconstruction: Post-occupancy case studies from Sri Lanka (2010)

After the 2004 Indian Ocean Tsunami, major housing reconstruction programmes were implemented in the affected countries. Five years after, the houses that were built offer post-occupancy lessons. This paper is based on Sri Lanka, one of the countries most severely impacted by the tsunami. The experience of these large reconstruction programmes prompts a fundamental question: what design and planning features should be encouraged, and what should be avoided, in postdisaster housing reconstruction programmes? Based on primary information from interviews of community members and representatives of agencies involved in planning and implementing the programmes, three main aspects are discussed - design, construction and post-occupancy issues. From the micro-scale of design of spaces within the house to the urban scale of settlement layout, the achievements made in the context of a highly constrained post-disaster situation as well as issues that required more careful attention are discussed here. The paper underscores the importance of resilience and capacity for adaptation of the communities in these settlements, and highlights the necessity of involving local communities in all stages of housing reconstruction.
Infrastructure Planning for Electric Vehicles with Battery Swapping (2013)

Electric vehicles (EVs) have been proposed as a key technology to help cut down the massive greenhouse gas emissions from the transportation sector. Unfortunately, because of the limited capacity of batteries, typical EVs can only travel for about 100 miles on a single charge and require hours to be recharged. The industry has proposed a novel solution centered around the use of “swapping stations,” at which depleted batteries can be exchanged for recharged ones in the middle of long trips. The possible success of this solution hinges on the ability of the charging service provider to deploy a cost-effective infrastructure network, given only limited information regarding adoption rates. We develop robust optimization models that aid the planning process for deploying battery-swapping infrastructure. Using these models, we study the potential impacts of battery standardization and technology advancements on the optimal infrastructure deployment strategy. This paper was accepted by Dimitris Bertsimas, optimization.
Biscrolling Nanotube Sheets and Functional Guests into Yarns (2011)

Multifunctional applications of textiles have been limited by the inability to spin important materials into yarns. Generically applicable methods are demonstrated for producing weavable yarns comprising up to 95 weight percent of otherwise unspinnable particulate or nanofiber powders that remain highly functional. Scrolled 50-nanometer-thick carbon nanotube sheets confine these powders in the galleries of irregular scroll sacks whose observed complex structures are related to twist-dependent extension of Archimedean spirals, Fermat spirals, or spiral pairs into scrolls. The strength and electronic connectivity of a small weight fraction of scrolled carbon nanotube sheet enables yarn weaving, sewing, knotting, braiding, and charge collection. This technology is used to make yarns of superconductors, lithium-ion battery materials, graphene ribbons, catalytic nanofibers for fuel cells, and titanium dioxide for photocatalysis.