gebhart/patrepeval huggingface dataset:
Effect of growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments (1995)

Growth factors have been shown to stimulate fibroblast division and thus may influence ligament healing. We analyzed the effects of individual growth factors on the proliferation of fibroblasts from the medial collateral and anterior cruciate ligaments of the rabbit in vitro in order to identify growth factors that might enhance proliferation of fibroblasts and to compare the responses of the fibroblasts from the two ligaments to these growth factors. Through measurement of the uptake of [3H]-thymidine into DNA, fibroblasts from these ligaments that had been treated with epidermal growth factor and basic fibroblast growth factor were found to proliferate nearly eight times more than control fibroblasts. Additionally, the fibroblasts of both ligaments proliferated at similar rates when exposed to platelet-derived growth factor-AA, platelet-derived growth factor-BB, basic fibroblast growth factor, insulin-like growth factor-1, and interleukin-1-alpha. However, epidermal growth factor and transforming growth factor-beta caused the fibroblasts from the medial collateral ligament to proliferate at a rate 1.3-1.4 times greater than that of fibroblasts from the anterior cruciate ligament. The reverse was true with acidic fibroblast growth factor, which stimulated the fibroblasts from the anterior cruciate ligament to proliferate at a rate 1.3-1.6 times greater than that of fibroblasts from the medial collateral ligament. This study demonstrated that growth factors can stimulate cell division in ligaments and may be effective in enhancing ligament healing but that these differences were not great enough to explain fully the clinical differences observed between healing of the medial collateral and anterior cruciate ligaments.
Lymphatic vessels in vascularized human corneas: immunohistochemical investigation using LYVE-1 and podoplanin. (2002)

Immunohistochemistry with novel lymph-endothelium markers and ultrastructural analyses indicate the existence of lymphatic vessels in vascularized human corneas. Human corneal lymphangiogenesis appears to be correlated with the degree of corneal hemangiogenesis and may at least partially be mediated by VEGF-C and its receptor VEGFR3.
[Basic studies on oral administration of lentinan (I)--influence on lymphocyte subsets in peripheral venous blood]. (1989)

The effect of oral administration of lentinan (LTN), a biological response modifier, in the control of systemic immune function was studied in 6-week old male Wistar-Imamichi SPF rats. In the LTN group, 1 mg LTN dissolved in 1 ml physiological saline was administration forcibly into the stomach twice weekly. Physiological saline alone was administered in a similar fashion to the control group. Blood samples were obtained prior to and after four and eight weeks of administration. White blood cells and lymphocyte counts were obtained and lymphocyte subsets were measured using monoclonal antibodies W3/13, W3/25 and 0 X 8 (Sera-Lab), and a laser flow cytometry system (Orthospectrum III, Orthodiagnostic System). The T cell ratio, helper/inducer T (Th) cell ratio, and suppressor/cytotoxic T (Ts) cell ratio were measured. The peripheral white blood cell count and lymphocyte count were not significantly different between the control and LTN groups. After four weeks of LTN administration, however, the LTN group showed a significantly higher T cell ratio, Th cell ratio and Th/Ts cell ratio than did the control group, and the Ts cell ratio was significantly lower. In the groups undergoing administration for eight weeks, no difference was noted in the lymphocyte subsets between the two groups. Oral administration of LTN apparently modulates the systemic immune function through T cell stimulation, especially Th cells, but continued administration may induce a tolerance to the effect of LTN.
Ion Channels in Innate and Adaptive Immunity (2015)

Ion channels and transporters mediate the transport of charged ions across hydrophobic lipid membranes. In immune cells, divalent cations such as calcium, magnesium, and zinc have important roles as second messengers to regulate intracellular signaling pathways. By contrast, monovalent cations such as sodium and potassium mainly regulate the membrane potential, which indirectly controls the influx of calcium and immune cell signaling. Studies investigating human patients with mutations in ion channels and transporters, analysis of gene-targeted mice, or pharmacological experiments with ion channel inhibitors have revealed important roles of ionic signals in lymphocyte development and in innate and adaptive immune responses. We here review the mechanisms underlying the function of ion channels and transporters in lymphocytes and innate immune cells and discuss their roles in lymphocyte development, adaptive and innate immune responses, and autoimmunity, as well as recent efforts to develop pharmacological inhibitors of ion channels for immunomodulatory therapy.
Cholesterol and Coronary Heart Disease: A Perspective (1985)

In this issue ofTHE JOURNALis the statement of the National Institutes of Health Consensus Development Conference on lowering blood cholesterol<sup>1</sup>; their recommendations are reasonable and can be supported. However, it is important at this point to review certain related issues. There is no doubt that elevated blood cholesterol levels and other "high-risk" factors, such as cigarette smoking, high blood pressure, obesity, physical inactivity, male sex, stressful lifestyle, and strong family history, are associated with an increased risk of development of coronary artery disease. However, even if all the recognized risk factors were eliminated except for the male sex, coronary atherosclerosis and death from coronary artery disease will<i>not</i>disappear. Therefore, it needs to be recognized that we do<i>not</i>yet know the cause(s) of coronary atherosclerosis<sup>2</sup>and there is a need for continued research to discover the cause(s) and specific treatment of coronary atherosclerosis. The recent interest,
InP DHBT technology and design methodology for high-bit-rate optical communications circuits (1998)

High-bit-rate optical communication links require high performance circuits. Electrical time division multiplex (ETDM) single channel bit-rate of 40 Gb/s is at hand, due to recent progress in both technology and design methodology. Multilevel modulation format can be envisaged for ETDM transmission. An InP double heterojunction bipolar transistor technology is presented in this paper. The methodology used and tools developed with optical communications in mind are also discussed. Fabricated circuits are reported: 40 Gb/s multiplexer and demultiplexer, a 20 Gb/s driver, a 30 Gb/s selector-driver, a 22 Gb/s decision circuit, and a decision-decoding circuit for multilevel transmissions.
KALMAN FILTERING FOR RESOLUTION OF OVERLAPPING LINES IN INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION-SPECTROMETRY (1992)

A Kalman filter was developed for resolving overlapping lines in inductively coupled plasma atomic emission spectrometry (ICP-AES) and evaluated experimentally with the determination of La in the presence of Ho, and Cu in the presence of Pr. The whiteness of the innovation sequence for an optimal filter was explored to be the criterion for the correction of the wavelength positioning errors which may occur in spectral scans. Under the conditions of the medium-resolution spectrometer and 1.5 pm step size in scans, the filter effectively resolved the Cu/Pr line pair having a small peak separation of 4.8 pm. For the La/Ho line pair with a peak distance of 9.8 pm, an unbiased estimate for La concentration was still obtained even when the signal-to-background ratio was down to 0.048. Favourable detection limits for real samples were achieved. Unstructured backgrounds were modeled theoretically and all spectral scans therefore did not require the correction for solvent.
Permanent photoinduced birefringence in a Ge-doped fiber (1991)

We report the observation of permanent photoinduced birefringence in a Ge-doped fiber exposed to cw mode locked at 532 nm. This effect is enhanced by increasing the Ge concentration in the core of the fiber, or by submitting it to a thermal hydrogen treatment. The photoinduced birefringence is however very weak, or nonexistent, with irradiation by 514 or 488 nm cw argon laser light.
Depression and Risk for Alzheimer Disease (2006)

A history of depression may confer an increased risk for later developing AD. This relation may reflect an independent risk factor for the disease.
Purification of ricin A1, A2, and B chains and characterization of their toxicity. (1986)

This paper describes a protocol for the preparation of highly purified A (A1 and A2) and B chains of the plant toxin, ricin, and biochemical and biological characterization of these proteins. Intact ricin was bound to acid-treated Sepharose 4B and was split on the column into A and B chains with 2-mercaptoethanol. The A chains were eluted with borate buffer containing 2-mercaptoethanol. A1 and A2 were then partially separated by cation exchange chromatography and the contaminating B chain was removed by affinity chromatography on Sepharose-asialofetuin and Sepharose-monoclonal anti-B chain. The B chain was eluted from the Sepharose 4B column by treatment with galactose and was further purified by cation and anion exchange chromatography; contaminating A chains were removed by affinity chromatography on Sepharose-monoclonal anti-A chain. The purified A and B chains were active as determined by their ability to inhibit protein synthesis in a cell-free assay and their binding to asialofetuin, respectively. Furthermore, by polyacrylamide gel electrophoresis, toxicity in mice, and toxicity on several different cell types, both A and B chains were shown to be minimally cross-contaminated. Finally, it was shown that ammonium chloride significantly enhanced the nonspecific toxicity of B chains for cells in vitro. In contrast, ammonium chloride did not enhance either the nonspecific toxicity of A chains in vitro or the specific toxicity of A chain-containing immunotoxins prepared with the highly purified A1, A2 chains.
Induction of neurite outgrowth by a conditioned-medium factor bound to the culture substratum (1978)

Heart-cell conditioned medium (HCM) induces rapid neurite outgrowth from isolated neurons in culture. The following evidence indicates that this action of HCM is due to a trypsin-sensitive factor which attaches to the polyornithinecoated culture substratum: (i) Pretreatment of the culture substratum with HCM allows rapid neurite outgrowth to occur even in unconditioned media. The active factor remains bound to the substratum during the period of neurite outgrowth. (ii) The substratum-bound activity is destroyed by trypsin treatment, but is insensitive to collagenase, RNase, and DNase. (iii) The factor that binds to the substratum is essential for neurite outgrowth, because HCM is no longer active when the material that binds to the polyornithine substratum has been removed by passage of the HCM over a series of culture dishes. However, this "depleted" HCM is still able to support the growth of nonneuronal cells. (iv) Most significantly, when neurons are cultured in whole HCM, the extent of neurite outgrowth is proportional to the amount of substratum-bound activity and not to the amount in solution, indicating that the substratum-bound form of the factor is more active. Previous observations [Collins, F. (1978) Dev. Biol. 65, 50-57] suggest that HCM promotes neurite outgrowth by increasing the adhesion between nerve cell surface extensions and the polyornithine-coated culture substratum. It is possible, therefore, that the factor in HCM that binds to the substratum possesses sites to which nerve cell surface components adhere.
In vivo uptake of lecithin-coated polystyrene beads by rat hepatocytes and sinusoidal endothelial cells (1996)

Uptake of 240 nm lecithin-coated polystyrene beads was observed by Kupffer cells, endothelial cells and hepatocytes. These beads were considered to be transported across the endothelial cells by transcytosis. Pseudopodia and bristle-coated invaginations were not employed by the hepatocytes when incorporating the beads.
Use of Anther‐Derived Haploids in Nicotiana. I. Isolation of Breeding Lines Differing in Total Alkaloid Content1 (1974)

A large population of haploid Nicotiana tabacum plants was obtained from the F 1 hybrid ‘LA Burley 21’ ✕ ‘Burley 21’ using an anther culture technique. LA Burley 21 is homoyzgous recessive (aabb) for total alkaloid content, and Burley 21 is homozygous dominant (AABB). Haploid genotypes for alkaloid content included AB, Ab, aB, and ab, obtained from the diploid F 1 hybrid of genotype AaBb. The haploid plants were verified cytologically, and individual haploids were chromosomally doubled by either root or leaf in vitro tissue culture procedures to recover the four homozygous doubled haploid forms. The two parental genotypes (AABB and aabb) and the low‐intermediate (aaBB) and high‐intermediate (AAbb) genotypes were evaluated for stability and compared with conventionally derived alkaloid lines. Haploid plants were extracted from doubled haploid genotypes to obtain additional evidence on stability of anther‐derived haploids. The haploids and their derivatives behave as stable true‐breeding genotypes. The evidence presented supports the hypothesis that two major genes control total alkaloid production in burley tobacco. The feasibility of using the anther‐derived haploid technique for the development of tobacco breeding lines is demonstrated.
Decoding the Entry of Two Novel Cell-Penetrating Peptides in HeLa Cells:  Lipid Raft-Mediated Endocytosis and Endosomal Escape (2004)

Cellular entry of peptide, protein, and nucleic acid biopharmaceuticals is severely impeded by the cell membrane. Linkage or assembly of such agents and cell-penetrating peptides (CPP) with the ability to cross cellular membranes has opened a new horizon in biomedical research. Nevertheless, the uptake mechanisms of most CPP have been controversially discussed and are poorly understood. We present data on two recently developed oligocationic CPP, the sweet arrow peptide SAP, a gamma-zein-related sequence, and a branched human calcitonin derived peptide, hCT(9-32)-br, carrying a simian virus derived nuclear localization sequence in the side chain. Uptake in HeLa cells and intracellular trafficking of N-terminally carboxyfluorescein labeled peptides was studied by confocal laser scanning microscopy and flow cytometry using biochemical markers in combination with quenching and colocalization approaches. Both peptides were readily internalized by HeLa cells through interaction with the extracellular matrix followed by lipid raft-mediated endocytosis as confirmed by reduced uptake at lower temperature, in the presence of endocytosis inhibitors and through cholesterol depletion by methyl-beta-cyclodextrin, supported by colocalization with markers for clathrin-independent pathways. In contrast to the oligocationic SAP and hCT(9-32)-br, interaction with the extracellular matrix, however, was no prerequisite for the observed lipid raft-mediated uptake of the weakly cationic, unbranched hCT(9-32). Transient involvement of endosomes in intracellular trafficking of SAP and hCT(9-32)-br prior to endosomal escape of both peptides was revealed by colocalization and pulse-chase studies of the peptides with the early endosome antigen 1. The results bear potential for CPP as tools for intracellular drug delivery.
Epicardial boundary detection using fuzzy reasoning (1991)

A fully automated system for detecting the endocardial and epicardial boundaries in a two-dimensional echocardiography by using fuzzy reasoning techniques is proposed. The image is first enhanced by applying the Laplacian-of-Gaussian edge detector. Second, the center of the left ventricle is determined automatically by analyzing the original image. Next, a search process radiated from the estimated center is performed to locate the endocardial boundary by using the zero-crossing points. After this step, the estimation of the range of radius of a possible epicardial boundary is carried out by comparing the high-level knowledge of intensity changes along all directions with the actual image intensity changes. The high-level knowledge of global intensity change in the image is acquired from experts in advance, and is represented in the form of fuzzy linguistic descriptions and relations. Knowledge of local intensity change can therefore be deduced from the knowledge of global intensity change through fuzzy reasoning.
Constitutive inhibitory action of muscarinic receptors on adenylyl cyclase in cardiac membranes and its stereospecific suppression by hyoscyamine. (2002)

Muscarinic acetylcholine receptors in the heart have been shown to display agonist-independent spontaneous (constitutive) activity which causes changes in the opening of cardiac ion channels and in the activity of G proteins. We investigated whether an inhibition of the constitutive activity of muscarinic receptors induced by the binding of antagonist brings about a change in the synthesis of cyclic AMP in rat cardiac membranes, and whether the action ofthe antagonist is stereospecific. Atropine and S-(-)-hyoscyamine were indeed found to enhance the forskolin-stimulated synthesis of cyclic AMP in rat cardiac (both atrial and ventricular) membranes by up to 24%. The effect was stereospecific and the potency of R-(+)-hyoscyamine was 30 fold lower than that of the S-(-) enantiomer, confirming that the action of hyoscyamine is receptor-mediated. The effect did not depend on the presence of endogenous acetylcholine in the system used. The results strongly suggest that the adenylyl cyclase in the heart is exposed to continuous mild inhibition by constitutively active muscarinic receptors in the membranes of cardiomyocytes.
A0C: Alpha Zero in Continuous Action Space (2018)

A core novelty of Alpha Zero is the interleaving of tree search and deep learning, which has proven very successful in board games like Chess, Shogi and Go. These games have a discrete action space. However, many real-world reinforcement learning domains have continuous action spaces, for example in robotic control, navigation and self-driving cars. This paper presents the necessary theoretical extensions of Alpha Zero to deal with continuous action space. We also provide some preliminary experiments on the Pendulum swing-up task, empirically showing the feasibility of our approach. Thereby, this work provides a first step towards the application of iterated search and learning in domains with a continuous action space.
A Multiplexed Protein Kinase Assay (2007)

We report a novel protein kinase assay designed for high-throughput detection of one or many kinases in a complex mixture. A solution-phase phosphorylation reaction is performed on 900 different peptide substrates, each covalently linked to an oligonucleotide tag. After incubation, phosphoserine, phosphothreonine, and phosphotyrosine are chemically labeled, and the substrates are hybridized to a microarray with oligonucleotides complementary to the tags to read out the phosphorylation state of each peptide. Because protein kinases act on more than one peptide sequence, each kinase can be characterized by a unique signature of phosphorylation activity on multiple substrates. Using this method, we determined signatures for 26 purified kinases and demonstrated that enzyme mixtures can be screened for activity and selectivity of inhibition.
ASC is essential for LPS‐induced activation of procaspase‐1 independently of TLR‐associated signal adaptor molecules (2004)

Toll-like receptors (TLRs) initiate a signalling cascade via association with an adaptor molecule, myeloid differentiation factor 88 (MyD88) and/or TIR domain-containing adaptor inducing-IFN-beta (Trif), to induce various pro-inflammatory cytokines for microbial eradication. After stimulation of TLR4 with lipopolysaccharide (LPS), both IL-1beta and IL-18 are processed, depending on the activation of caspase-1, although its mechanism remains unclear. ASC is an adapter protein possibly involved in the activation of procaspase-1. To unravel the requirement of ASC, we generated Asc(-/-) mice. Upon stimulation with LPS, Asc(-/-) macrophages failed in the processing of procaspase-1 and maturation of pro-IL-1beta and pro-IL-18, but normally produced other pro-inflammatory cytokines including TNF-alpha and IL-6. MyD88(-/-) and Trif(-/-) macrophages showed normal activation of caspase-1, demonstrating a dispensable role for MyD88 and Trif. After, LPS-challenged Asc(-/-) mice lacked serum elevation of IL-1beta and IL-18. Moreover, the Asc(-/-) mice exhibited neither acute liver injury nor lethal shock. These results demonstrate critical roles for ASC in the release of IL-1beta/IL-18 via activation of caspase-1 and provide new insights into the inflammatory responses for host defence and diseases.
35.3: Distinguished Paper : A Novel Self‐Aligned Top‐Gate Oxide TFT for AM‐OLED Displays (2011)

Abstract We developed a novel self‐aligned top‐gate oxide TFT for AM‐OLED displays. Our developed Al reaction method to obtain source/drain regions is effective to fabricate a short channel and highly reliable TFT. A 9.9‐inch diagonal qHD AM‐OLED display was demonstrated to provide applicable solution for a large‐sized and ultra‐high definition OLED mass production.
Blends of CO2 and 2-Ethyl Hexanol as Replacement Foaming Agents for Extruded Polystyrene (2001)

By the year 2010, HCFC 142b will be banned for use as a foaming agent for extruded polystyrene (PS) foam in North America. Many blends of foaming agents have been patented as replacements to expand PS. In this study the optimal concentration of a previously unexplored blend of CO2 and 2-ethyl hexanol (2-EH) is shown to allow the production of PS foam of 30 kg/m3 density. The glass transition temperature reduction of the PS, due to the incorporation of 2-EH, is believed to be an important contributor to the success of this foaming agent blend. In long-term use of the foams, the 2-EH does not measurably diffuse out of the PS, whereas the half-life of CO2 diffusion out of the foam is measured in weeks.
Hemolysis of human erythrocytes by transient electric field. (1977)

Exposure of human erythrocytes, under isotonic conditions, to a high voltage pulse of a few kV/cm leads to total hemolysis of the red cells. Experiments described herein demonstrate that the hemolysis is due to the effect of electric field. Neither the effect of current nor the extent of the rapid Joule-heating to the suspending medium shows a direct correlation with the observed hemolysis. Voltage pulsation of the erythrocyte suspension can induce a transmembrane potential across the cell membrane and, at a critical point, it either opens up or creates pores in the red cells. In isotonic saline the pores are small. They allow passage of potassium and sodium ions but not sucrose and hemoglobin molecules. The pores are larger in low ionic conditions and permit permeation of sucrose molecules, but under no circumstances can hemoglobin leak out as the direct result of the voltage pulse. Kinetic measurements indicate that the hemolysis of the red cells follows a stepwise mechanism: leakage of ions leads to an osmotic imbalance which in turn causes a colloidal hemolysis of the red cells. Other effects of the voltage pulsation are also discussed.