gebhart/patrepeval huggingface dataset:
Glucosamine-sensitive and -insensitive detritiation of [2-3H]glucose in isolated rat hepatocytes: a study of the contributions of glucokinase and glucose-6-phosphatase (1995)

Glucosamine, a potent inhibitor of glucokinase (hexokinase IV or D), was used to estimate the contribution of this enzyme to glucose phosphorylation in freshly isolated rat hepatocytes and its sensitivity to fructose 6-phosphate in situ. Experiments with radiolabelled glucosamine indicated that this amino sugar, at concentrations of 5 or 40 mM, readily penetrated hepatocytes to reach in 1 min a total (i.e., glucosamine+metabolites) intracellular concentration equal to 0.8-1.2-fold its extracellular concentration. In marked contrast, N-acetylglucosamine barely penetrated the cells. The detritiation of [2-3H]glucose, used to estimate glucose phosphorylation in intact cells, was inhibited by glucosamine much more potently than by N-acetylglucosamine, half-maximal effects being reached at about 2.5 and 30 mM respectively. Extrapolation of the data indicated that about 12% of the detritiation was resistant to glucosamine. Dihydroxyacetone (10 mM), lactate (10 mM) + pyruvate (1 mM), and glucagon (1 microM) increased up to 8-fold the concentration of hexose 6-phosphates (glucose 6-phosphate+fructose 6-phosphate) and, against expectations, modestly decreased the detritiation rate measured in the absence of glucosamine. In the presence of 40 mM glucosamine, these agents increased the detritiation rate, which then positively correlated with the concentration of hexose 6-phosphates. This hexose 6-phosphates-dependent detritiation was sensitive to inhibition by vanadate, and was also catalysed by gel-filtered cell-free extracts, as well as by liver microsomes in the presence of phosphoglucoisomerase; it can be explained by an exchange reaction catalysed by glucose-6-phosphatase. When this exchange reaction is taken into account, it appears that the rate of glucose detritiation attributable to glucokinase decreases when the concentration of hexose 6-phosphates increases. This is in agreement with the known effect of fructose 6-phosphate to potentiate the inhibition of glucokinase by its regulatory protein.
Detection of the electromechanical delay and its components during voluntary isometric contraction of the quadriceps femoris muscle (2014)

Electromechanical delay (EMD) was described as a time elapsed between first trigger and force output. Various results have been reported based on the measurement method with observed inconsistent results when the trigger is elicited by voluntary contraction. However, mechanomyographic (MMG) sensor placed far away on the skin from the contracting muscle was used to detect muscle fiber motion and excitation-contraction (EC) coupling which may give unreliable results. On this basis, the purpose of this study was to detect EMD during active muscle contraction whilst introducing an ultrafast ultrasound (US) method to detect muscle fiber motion from a certain depth of the muscle. Time delays between onsets of EMG-MMG, EMG-US, MMG-FORCE, US-FORCE, and EMG-FORCE were calculated as 20.5 ± 4.73, 28.63 ± 6.31, 19.21 ± 6.79, 30.52 ± 8.85, and 49.73 ± 6.99 ms, respectively. Intrarater correlation coefficient (ICC) was higher than MMG when ultrafast US was used for detecton of the Δt EMG-US and Δt US-FORCE, ICC values of 0.75 and 0.70, respectively. Synchronization of the ultrafast ultrasound with EMG and FORCE sensors can reveal reliable and clinically useful results related to the EMD and its components when muscle is voluntarily contracted. With ultrafast US, we detect onset from the certain depth of the muscle excluding the tissues above the muscle acting as a low-pass filter which can lead to inaccurate time detection about the onset of the contracting muscle fibers. With this non-invasive technique, understanding of the muscle dynamics can be facilitated.
Factors influencing Agrobacterium‐mediated transient expression of uidA in wheat inflorescence tissue (2001)

A critical step in the development of Agrobacterium tumifaciens-mediated transformation is the establishment of optimal conditions for T-DNA delivery into tissue from which whole plants can be regenerated. The efficient transformation of inflorescence tissue from 'Baldus', a commercial wheat variety, using the Agrobacterium strain AGLI harbouring the binary vector pAL156 is reported here. The effects of various factors on delivery and the transient expression of the uidA gene were studied including the duration of preculture, vacuum infiltration, the effect of sonication treatments, and Agrobacterium cell density. Optimal T-DNA delivery (as measured by uidA activity) was obtained from inflorescence tissues precultured for 21 d and sonicated. Increasing Agrobacterium cell density, the duration of inoculation/co-cultivation, and vacuum pressure, up to a threshold, increased uidA expression. The investigation of factors that influence T-DNA delivery is an important first step in the utilization of Agrobacterium in the transformation of immature wheat inflorescence tissue.
Vitamin A as an immunomodulating agent. (1993)

Findings on the benefits and mechanism of action of vitamin A in measles and other infectious diseases and immunocompromised states are discussed. Vitamin A deficiency is one of the world's major malnutrition problems and is most commonly found in children under the age of five years. An association between vitamin A status and immune function has been suggested by community studies and animal experiments. Mortality and susceptibility to infection and diarrhea are higher in children with vitamin A deficiency. The association between increased mortality and morbidity and vitamin A deficiency is strongest in children with measles. Vitamin A supplementation reduces mortality and complications resulting from measles. Measles may increase the body's utilization of vitamin A, possibly because of the rapid destruction of epithelial surfaces. Vitamin A may boost immune responses in the elderly, persons with high exposure to ultraviolet light, patients who have undergone surgery, and persons with parasitic infection, but more studies are needed. The immune defect caused by vitamin A deficiency may be due to alterations in the glycoproteins of the lymphocyte membrane, an adverse effect on helper T-cell function, the effect on epithelial tissue, or some other mechanism. Vitamin A therapy is relatively safe, and its effectiveness in children with measles and possibly other groups appears to justify public health campaigns to eliminate vitamin A deficiency. Vitamin A apparently has important immunomodulating properties, notably in patients with measles.
SDN and OpenFlow for Converged Access/Aggregation Networks (2013)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text H. Woesner and D. Fritzsche, "SDN and OpenFlow for Converged Access/Aggregation Networks," in Optical Fiber Communication Conference/National Fiber Optic Engineers Conference 2013, OSA Technical Digest (online) (Optical Society of America, 2013), paper OTu3E.4. Export Citation BibTex Endnote (RIS) HTML Plain Text Get Video Citation alert Save article
Redox mechanism as alternative to ligand binding for receptor activation delivering disregulated cellular signals. (1994)

Cross-linking with specific ligand is a general requirement for ordered activation of cell surface receptors. In this study we demonstrated a novel pathway for disregulated receptor activation through a redox mechanism. Treatment of murine thymocytes or spleen cells with thiol-reactive HgCl2, a known inducer of autoimmune proliferative lymphocyte disorders in rodents, was found to induce tyrosine phosphorylation of several cellular proteins, which was up to 100 times as extensive as that triggered by stimulation with antireceptor antibody or mitogen. Through the cross-linkage by thiol-reactive bivalent mercury, transmembrane CD4, CD3, and CD45 and glycosylphosphatidylinositol-anchored Thy-1 were aggregated together on thymocytes or T lymphocytes. Along with the aggregation of Thy-1 and CD4, nonreceptor protein tyrosine kinase p56lck was aggregated and activated. These events were linked to extensive protein tyrosine phosphorylation, which was visualized as a well localized spot beneath the membrane. Under appropriate conditions, this novel pathway of multiple receptor aggregation delivered a disregulated signal into T lymphocytes, which cross-talked to the antireceptor antibody-induced signal, for prolonged cell proliferation and IL-2 production. These results suggest a novel mechanism of disregulation of the ligand-dependent receptor function.
Rheumatoid Arthritis: An Overview of New and Emerging Therapies (2005)

Rheumatoid arthritis (RA) is a chronic, inflammatory, systemic autoimmune disorder characterized by symmetric inflammation of synovial joints leading to progressive erosion of cartilage and bone. The aim of treatment is to mitigate joint destruction, preserve function, and prevent disability. The American College of Rheumatology guidelines for the treatment of RA recommend that newly diagnosed patients with RA begin treatment with disease-modifying antirheumatic drugs (DMARDs) within 3 months of diagnosis. Methotrexate remains the most commonly prescribed DMARD and is the standard by which recent new and emerging therapies are measured. Increasing knowledge regarding the immunologic basis of RA and advances in biotechnology have resulted in new, targeted biological therapies against proinflammatory cytokines that have dramatically changed the treatment paradigm and outcomes of patients with RA. This article reviews the pharmacological rationale underlying RA therapy, with a focus on currently available biological therapies and new therapies in development.
Regulation of Leishmania populations within the host. II. genetic control of acute susceptibility of mice to Leishmania donovani infection. (1977)

The acute growth rates of Leishmania donovani populations in twenty-five inbred mouse strains fall into two distinct groups: the susceptible (S) and the resistant (R). Hybrids within either category resemble their parents in susceptibility. Hybrids between categories are moderately resistant. Back-crossing of F1 hybrids to R and S parents, and interbreeding the F1 generation, give susceptibility ratios consistent with single gene control of acute susceptibility to visceral leishmaniasis. The distribution of this character among inbred mouse strains does not correspond to any well-studied gene nor does it appear to be linked to the H2 histocompatibility locus.
Impulse noise performance of an asymmetric digital subscriber lines passband transmission system (1995)

This letter presents an analytical method to numerically evaluate the bit error Probability (BEP) performance of a quadrature amplitude modulation (QAM) transmission system in the presence of impulse noise. This work facilitates characterization of the two-wire twisted-pair loop plant for high-speed digital communications. Simulation results are presented to demonstrate the effectiveness of this approach using parameters that are typical of the recently proposed Asymmetric Digital Subscriber Lines (ADSL) for high-speed digital transmission over local copper telephone loops.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Testing the Honeywell Durafet® for seawater pH applications (2010)

We report on the first seawater tests at 1 atm of the Honeywell Durafet® pH sensor, a commercially available ion sensitive field effect transistor (ISFET). Performance of this sensor was evaluated in a number of different situations including a temperature‐controlled calibration vessel, the MBARI test tank, shipboard underway mapping, and a surface mooring. Many of these tests included a secondary reference electrode in addition to the internal reference supplied with the stock Durafet sensor. We present a theoretical overview of sensor response using both types of reference electrode. The Durafet sensor operates with a short term precision of ± 0.0005 pH over periods of several hours and exhibits stability of better than 0.005 pH over periods of weeks to months. Our tests indicate that the Durafet pH sensor operates at a level of performance satisfactory for many types of biogeochemical studies at low pressure.
Myostatin and the control of skeletal muscle mass (1999)

The mechanisms by which tissue size is controlled are poorly understood. Over 30 years ago, Bullough proposed the existence of chalones, which act as tissue-specific negative growth regulators. The recent discovery of myostatin suggests that negative regulation of tissue growth may be an important mechanism for controlling skeletal muscle mass and raises the possibility that growth inhibitors may also be involved in regulating the size of other tissues.
Exercise and Sleep: Body-Heating Effects (1983)

Eight physically trained subjects underwent three experimental conditions on separate occasions between 1400 and 1800 h. Two conditions acted as controls for a high-intensity exercise (HI) condition of treadmill running at 80% VO2 max for a total of 80 min. The rate of body heating was modelled in a no-exercise passive heating condition (PH), and the total exercise load was replicated in a low-intensity condition (LI) at 40% VO2 max for 160 min. LI produced no slow-wave sleep (SWS--stages 3 + 4 sleep) changes, but was the only condition to produce significant increases in sleep length and in non-rapid eye movement (REM) sleep (stages 1 + 2 + 3 + 4), and a significant decrease in sleep onset time. Although HI and PH produced similar SWS increases, these consisted of significant increases in stage 3 sleep for HI and in stage 4 sleep for PH. No REM sleep parameter was affected under any condition. Self-estimates of presleep tiredness produced no significant findings. It was concluded that a high and sustained rate of body heating for 1-2 h, particularly the inherent rapid rates of core temperature increase and of body dehydration, may trigger a SWS response, and that exercise may simply be a vehicle for these effects.
Lecithin: Cholesterol Acyltransferase Expression Has Minimal Effects on Macrophage Reverse Cholesterol Transport In Vivo (2009)

These results demonstrate that LCAT overexpression does not promote an increased rate of macrophage RCT. Although LCAT activity does become rate limiting in the context of complete LCAT deficiency, RCT is reduced by only 50% even in the absence of LCAT. These data suggest that macrophage RCT may not be as dependent on LCAT activity as has previously been believed.
Catalytically Reactive Ruthenium Intermediates in the Homogeneous Oxidation of Alcohols to Esters (1984)

Abstract Ru(η 4 ‐tetracyclone)(CO) 3 and [Ru(η 4 ‐tetracyclone)(CO) 2 ] 2 were prepared from Ru 3 (CO) 12 and diphenylacetylene. Their rate of oxidation of PhCH 2 OH are, respectively, 1.6 and 4.2 times faster than their precursor Ru 3 (CO) 12 . Both complexes, as well as others, were also found in the oxidation reaction mixture: PhCH 2 OH+ Ph 2 C 2 + Ru 3 (CO) 12 which generates benzyl benzoate. Ru(η 4 ‐tetracyclone)(CO) 2 is most probably the true catalytic species in the catalytic oxidation of alcohols to esters.
Fingerprinting the datacenter (2010)

Contemporary datacenters comprise hundreds or thousands of machines running applications requiring high availability and responsiveness. Although a performance crisis is easily detected by monitoring key end-to-end performance indicators (KPIs) such as response latency or request throughput, the variety of conditions that can lead to KPI degradation makes it difficult to select appropriate recovery actions. We propose and evaluate a methodology for automatic classification and identification of crises, and in particular for detecting whether a given crisis has been seen before, so that a known solution may be immediately applied. Our approach is based on a new and efficient representation of the datacenter's state called a fingerprint, constructed by statistical selection and summarization of the hundreds of performance metrics typically collected on such systems. Our evaluation uses 4 months of trouble-ticket data from a production datacenter with hundreds of machines running a 24x7 enterprise-class user-facing application. In experiments in a realistic and rigorous operational setting, our approach provides operators the information necessary to initiate recovery actions with 80% correctness in an average of 10 minutes, which is 50 minutes earlier than the deadline provided to us by the operators. To the best of our knowledge this is the first rigorous evaluation of any such approach on a large-scale production installation.
On Analysis of Pragmatic Vagueness from the Perspective of Relevance Theory (2009)

This paper attempts to study pragmatic vagueness in the framework of Relevance Theory, which puts its emphasis on how to explain the reasonable existence of pragmatic vagueness and how to infer the implicature of vagueness. Using vagueness in our daily life can make communication economical. That is the internal factor leading to the frequent use of vagueness in daily communication. In terms of relevance theory, communication is to find the optimization of effects with minimum efforts. Therefore, we can use the principle of relevance to infer the optimization relevance and get the informative intention.
Identification of a putative isoform of the Na,K-ATPase β subunit (1989)

We have isolated cDNA clones from rat brain and human liver encoding a putative isoform of the Na,K-ATPase beta subunit. The rat brain cDNA contains an open reading frame of 870 nucleotides coding for a protein of 290 amino acids with a calculated molecular weight of 33,412. The corresponding amino acid sequence shows 98% identity with its human liver counterpart. The proteins encoded by the rat and human cDNAs exhibit a high degree of primary sequence and secondary structure similarity with the rat Na,K-ATPase beta subunit. We have therefore termed the polypeptides these cDNAs encode a beta 2 subunit with the previously characterized rat cDNA encoding a beta 1 subunit. Analysis of rat tissue RNA reveals that the beta 2 subunit gene encodes a 3.4-kilobase mRNA which is expressed in a tissue specific fashion distinct from that of rat beta 1 subunit mRNA. Cell lines derived from the rat central nervous system shown to lack beta 1 subunit mRNA sequences were found to express beta 2 subunit mRNA. These results suggest that different members of the Na,K-ATPase beta subunit family may have specialized functions.
The Infectivity of Polyoma Virus DNA for Mouse Embryo Cells in the Presence of Diethylaminoethyl-dextran (1968)

SUMMARY Plaques were produced in mouse-embryo monolayers by infection with polyoma virus DNA in the presence of diethylaminoethyl-dextran. Optimum conditions for plaque assay were established and dose-response relationships for component I and component (II+III) polyoma DNA determined. Efficiencies up to 6 × 105 p.f.u./µg. were obtained for component I DNA.
Functional properties of antibody insulin-like growth factor fusion proteins. (1994)

Genetic engineering and expression techniques have been used to produce antibody growth factor fusion proteins. Insulin-like growth factors (IGFs) 1 and 2 have been fused to mouse-human chimeric IgG3 at the end of CH1, immediately after the hinge, and at the end of CH3. Fusion heavy chains of the expected molecular weight were expressed, assembled with a co-expressed light chain, and secreted. The resulting molecules continued to bind antigen; they also bound the growth factor receptors, albeit with decreased affinity. The molecule with IGF1 attached after CH3 (CH3-IGF1) had reduced ability to carry out complement-mediated cytolysis. In contrast the molecule with IGF2 attached after CH3 (CH3-IGF2) showed an approximately 50-fold increase in its ability to effect complement-mediated cytolysis and so should be an effective cytolytic agent. Both CH3-IGF1 and CH3-IGF2 bound Fc gamma RI with affinity similar to that of IgG3. The growth factor fusion proteins showed small but significant uptake into the brain parenchyma.
Specific B lymphocytes efficiently pick up, process and present antigen to T cells. (1985)

To study the nature of the antigen bridge between specific B and T lymphocytes we have isolated from the same donor clones of human T cells and EBV-transformed B cells specific for tetanus toxoid (TT). We found that TT-specific B cells are extremely efficient in presenting TT (but not an unrelated antigen) to T cells, as they can trigger T cell proliferation in the presence of only 10(-12) M TT, a concentration that is approximately 10(4) times lower than that required for presentation by conventional antigen-presenting cells such as macrophages or non-specific B cells. The high avidity of the interaction between specific B and T cells is reflected by the resistance to inhibition by antibodies against the T4 antigen. Experiments with fixed and chloroquine-treated B cells show that the role of surface antibodies is limited to the uptake of antigen and that in order to obtain presentation, the antigen has to be internalized, processed and subsequently displayed on the cell surface, where it becomes available for T cell recognition in an MHC-restricted fashion.
General Method for Site-Specific Protein Immobilization by Staudinger Ligation (2007)

Protein microarrays are playing an increasingly important role in the discovery and characterization of protein-ligand interactions. The uniform orientation conferred by site-specific immobilization is a demonstrable advantage in using such microarrays. Here, we report on a general strategy for fabricating gold surfaces displaying a protein in a uniform orientation. An azido group was installed at the C-terminus of a model protein, bovine pancreatic ribonuclease, by using the method of expressed protein ligation and a synthetic bifunctional reagent. This azido protein was immobilized by Staudinger ligation to a phosphinothioester-displaying self-assembled monolayer on a gold surface. Immobilization proceeded rapidly and selectively via the azido group. The immobilized enzyme retained its catalytic activity and was able to bind to its natural ligand, the ribonuclease inhibitor protein. This strategy provides a general means to fabricate microarrays displaying proteins in a uniform orientation.
A specific inhibitory effect of multivalent trehalose toward Aβ(1-40) aggregation (2011)

The inhibitory effect of disaccharides and their polyvalent compounds toward aggregation of Aβ(1-40) was investigated. Polyvalent trehalose, maltose and lactose were synthesized, and their inhibitory effects were investigated together with those of the corresponding monomeric disaccharides. The inhibitory effects of the monomeric disaccharides were weak, but were amplified by multivalency. Trehalose and polyvalent trehalose induced formation of aggregates with specific pseudo-spherical morphology, which did not occur in the presence of the other disaccharides or in the absence of additives. Addition of polyvalent trehalose neutralized the cytotoxicity of Aβ, while monomeric trehalose induced weak cytotoxicity (toward HeLa cells). The specific inhibitory effects of trehalose and polyvalent trehalose on Aβ aggregation are discussed in the terms of the conformation of those molecules.