gebhart/patrepeval huggingface dataset:
Measurement of arachidonate and its metabolites extracted from human normal and malignant gastrointestinal tissues. (1987)

This is the first report of human gastrointestinal arachidonate and prostanoids measured quantitatively by gas chromatography-mass spectrometry (GC-MS) in extracts of human cancers and macroscopically normal tissues from the stomach and colon. There were microgram/g amounts of arachidonate, and the particularly high yield from the tumours may explain why they usually produce more prostaglandins than the normal tissues in which they arise. There was only a small conversion of the arachidonate into prostanoids. 6-Keto-PGF1 alpha was the most abundant metabolite measured, particularly in the tumour extracts, with smaller amounts of prostaglandins E2, F2 alpha and D2.
Sequence and mutational analysis of ESS1, a gene essential for growth in Saccharomyces cerevisiae (1989)

A newly isolated gene, ESS1, was shown to encode a protein required for vegetative growth in Saccharomyces cerevisiae. The nucleotide sequence of ESS1 revealed a 172 amino acid open reading frame predicting a highly basic, 19.5 kilodalton product. Although the gene was isolated by cross-hybridization with the vertebrate v-sis oncogene, the primary amino acid sequence bears only a slight resemblance to the p28sis protein. ESS1 was shown to be single copy in the yeast genome and transcriptionally active during logarithmic growth. It is located on the right arm of chromosome X, 6 centimorgans distal to ilv3. The genetic map location indicates it is not allelic to any previously characterized mutation in this organism. Both inactivation of ESS1 by gene disruption and overexpression by fusion to a heterologous promoter were detrimental to growth in both haploid and diploid cell types. Under non-permissive conditions, the terminal phenotype of strains containing a suppressible amber mutation within ESS1 was one of aberrant multibudded structures. Examination of this morphology indicates that loss of ESS1 function may lead to a defect in cytokinesis or cell separation.
The Use of Housekeeping Genes (HKG) as an Internal Control for the Detection of Gene Expression by Quantitative Real-Time RT-PCR (2003)

Quantitative real-time RT-PCR is a very useful technique for estimating gene expression at the mRNA level. The expression of a tested gene has to be compared with that of a control gene. Various housekeeping genes have been used as control genes in different systems. In our study we tested several housekeeping genes in the model of gene expression after induction of apoptosis and differentiation. The myeloid cell lines were incubated with phorbol esters, butyric acid and combination of TNFalpha and IFNgamma to induce differentiation. Camptothecin was used for induction of apoptosis. Tested control genes included beta2-microglobulin, GAPDH, 18S ribosomal RNA and abl. GAPDH was found to be the best control gene in the apoptotic system. Different control genes were suitable for different systems where differentiation or senescence was induced. Our results show that attention should be paid to the choice of an appropriate control gene of quantitative real-time RT-PCR for different experimental models and various experimental conditions.
Effect of loperamide and naloxone on mouth-to-caecum transit time evaluated by lactulose hydrogen breath test. (1985)

The effect of loperamide and naloxone on mouth-to-caecum transit time was evaluated by the lactulose hydrogen breath test in four men and four women. Each subject underwent tests during the administration of placebo, loperamide (12-16 mg po), naloxone (40 micrograms/kg/h by a three-hour intravenous infusion), and loperamide plus naloxone, carried out at intervals of one or two weeks. The transit time was significantly longer after loperamide, and this effect was antagonised by the concomitant administration of naloxone whereas naloxone administered alone had no effect on mean transit time. No clinically important side effects were reported.
Potential therapeutic application of antisense oligonucleotides in the treatment of ocular diseases (2001)

Antisense oligonucleotides are a class of compounds being developed as therapeutic agents for many types of diseases. Although still relatively early in the clinical characterisation, the power of this technology lies in the ability to utilise genetic information and the known molecular mechanisms of disease to foster efficient and rational drug design. Consideration of novel approaches to treating ocular diseases is of interest because there are many ocular diseases with no satisfactory treatments. The recent availability of animal models of many ocular diseases provides the opportunity to use antisense oligonucleotides to understand the mechanisms of disease pathology and to potentially intervene therapeutically in ocular disease. There are already a number of examples where antisense oligonucleotides have been applied to the study of ocular physiology and disease and there is an antisense oligonucleotide approved for the treatment of cytomegalovirus (CMV) retinitis. We summarise current research in this area and highlight the properties of these compounds that are favourable for use as ocular therapeutics.
Sex hormones, inflammation and the metabolic syndrome: a population-based study (2003)

Low testosterone and SHBG levels were strongly associated not only with components of the metabolic syndrome, but also with the metabolic syndrome itself, independently of body mass index. Furthermore, sex hormones were associated with inflammation and body iron stores. Even in the absence of late-stage consequences such as diabetes and cardiovascular disease, subtle derangements in sex hormones are present in the metabolic syndrome, and may contribute to its pathogenesis.
The Ocular Hypertension Treatment Study (1999)

The intraocular pressure among enrolled subjects was sufficiently high to provide an adequate test of the potential benefit of ocular hypotensive medication in preventing or delaying glaucomatous damage. The large number of African American subjects enrolled should provide a good estimate of the African American response to topical medication.
Adherence of Human Vaginal Lactobacilli to Vaginal Epithelial Cells and Interaction with Uropathogens (1998)

Three strains of Lactobacillus, identified as Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus jensenii, were selected from among 70 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization or antagonism. All three self-aggregated and adhered to epithelial vaginal cells, displacing well-known vaginal pathogens, such as G. vaginalis, and inhibiting the growth in vitro of Escherichia coli and Streptococcus agalactiae. The surface components involved in self-aggregation appeared to be proteins for L. gasseri and lipoproteins for L. acidophilus and L. jensenii, as judged by susceptibility to treatment with appropriate degrading enzymes. The factors responsible for adherence to epithelial vaginal cells seemed to be glycoproteins (L. acidophilus and L. gasseri) and carbohydrate (L. jensenii). The receptors of the vaginal cells were glycolipids, which presumably were the targets of the competition observed between the lactobacilli and the pathogenic microbes.
IPv6--the new Internet protocol (1996)

1. Introduction. Preparing for a Decision. Two Years of Competition. The New Specifications. Points of Controversy. Further Reading. 2. The Design of IPv6. The IPv6 Header Format. From Options to Extension Headers. The Evolution of ICMP. Impact on the Upper Layers. Points of Controversy. Further Reading. 3. Routing and Addressing. Address Architecture. Multicasting and Anycasting. Inter-domain Routing. Intra-domain Routing. Points of Controversy. Further Reading. 4. Plug and Play. Autoconfiguration. Address Resolution. Advanced Features. Mapping to Specific Link Technologies. Points of Controversy. Further Reading. 5. Bringing Security to the Internet. Encryption and Authentication. Key Distribution. Usage of IPv6 Security. Points of Controversy. Further Reading. 6. Real-time Support and Flows. An Elusive Specification. Supporting Reservations. Hierarchical Coding and Priorities. Points of Controversy. Further Reading. 7. Transitioning the Internet. Dual-stack Strategy. Building the 6-Bone. Connecting End Stations. Early Deployment. Points of Controversy. Further Reading. 8. A Provisional Conclusion. The Right Solution. The Right Time. Milestones. The Future Will Tell. Glossary. Index.
Molecular cloning and characterization of a novel human ribonuclease (RNase k6): increasing diversity in the enlarging ribonuclease gene family (1996)

The discovery of Ribonuclease k6 (RNase k6) was an unexpected result of our ongoing efforts to trace the evolutionary history of the ribonuclease gene family. The open reading frame of RNase k6, amplified from human genomic DNA, encodes a 150 amino acid polypeptide with eight cysteines and histidine and lysine residues corresponding to those found in the active site of the prototype, ribonuclease A. The single-copy gene encoding RNase k6 maps to human chromosome 14 and orthologous sequences were detected in both primate and non-primate mammalian species. A single mRNA transcript (1.5 kb) was detected in all human tissues tested, with lung representing the most abundant source. At the cellular level, transcripts encoding RNase k6 were detected in normal human monocytes and neutrophils (but not in eosinophils) suggesting a role for this ribonuclease in host defense. Of the five previously identified human ribonucleases of this group, RNase k6 is most closely related to eosinophil-derived neurotoxin (EDN), with 47% amino acid sequence identity; slight cross-reactivity between RNase k6 and EDN was observed on Western blots probed with polyclonal anti-EDN antiserum. The catalytic constants determined, Km = 5.0 microM and Kcat = 0.13 s-1, indicate that recombinant RNase k6 has approximately 40-fold less ribonuclease activity than recombinant EDN. The identification and characterization of RNase k6 has extended the ribonuclease gene family and suggests the possibility that there are others awaiting discovery.
Evaluation of 99mTc-RP128 as a potential inflammation imaging agent: human dosimetry and first clinical results. (2001)

This study shows that 99mTc-RP128 is safe and can successfully be used to visualize clinically affected joints in patients with long-standing rheumatoid arthritis. A proposed radioactive dose of 450-500 MBq will produce an effective dose well within the range of effective doses for commonly used radiopharmaceuticals.
A Conversation-Mining System for Gathering Insights to Improve Agent Productivity (2007)

We describe a method to analyze transcripts of conversations between customers and agents in a contact center. The aim is to obtain actionable insights from the conversations to improve agent performance. Our approach has three steps. First we segment the call into logical parts. Next we extract relevant phrases within different segments. Finally we do two dimensional association analysis to identify actionable trends. We use real data from a contact center to identify specific actions by agents that result in positive outcomes. We also show that implementing the actionable results in improved agent productivity.
The morphological, optical and electrical properties of SnO 2 :F thin films prepared by spray pyrolysis (2008)

Abstract Combining the spray pyrolysis and the sol–gel techniques gives the possibility to produce Fluorine doped Tin oxide (SnO 2 :F) thin films. Transparent conducting SnO 2 :F thin films have been deposited on glass substrates by the spray pyrolysis technique. This technique for the fabrication of SnO 2 :F filmsby combining sol–gel process and the spray pyrolysis technique ispresented in this paper. The Sol–gel precursors have been successfully prepared using SnCl 2 ·5H 2 O and (Ac)F 3 . The structural, electrical, and optical properties of these films were investigated. The high resolution transmission electron microscopy (HRTEM) and selected area diffraction (SAD) patterns of SnO 2 :F films show that the gel films lead to a tetragonal structure. The X‐ray diffraction pattern of the films deposited at substrate temperature 530° , the orientation of the films was predominantly [110]. In addition, the surface chemical components were also examined by X‐ray photoelectron spectroscopy (XPS) showing the SnO 2 :F deposited with the atomic concentration ratios Sn/F 1.82:1. The minimum sheet resistance was 50 Ω and average transmission in the visible wavelength range of 300 to 800 nm was 87.25%. Copyright © 2008 John Wiley &amp; Sons, Ltd.
Three-dimensional subband coding with motion compensation (1994)

Three-dimensional (3-D) frequency coding is an alternative approach to hybrid coding concepts used in today's standards. The first part of this paper presents a study on concepts for temporal-axis frequency decomposition along the motion trajectory in video sequences. It is shown that, if a two-band split is used, it is possible to overcome the problem of spatial inhomogeneity in the motion vector field (MVF), which occurs at the positions of uncovered and covered areas. In these cases, original pixel values from one frame are placed into the lowpass-band signal, while displaced-frame-difference values are embedded into the highpass band. This technique is applicable with arbitrary MVF's; examples with block-matching and interpolative motion compensation are given. Derivations are first performed for the example of two-tap quadrature mirror filters (QMF's), and then generalized to any linear-phase QMF's. With two-band analysis and synthesis stages arranged as cascade structures, higher resolution frequency decompositions are realizable. In the second part of the paper, encoding of the temporal-axis subband signals is discussed. A parallel filterbank scheme was used for spatial subband decomposition, and adaptive lattice vector quantization was employed to approach the entropy rate of the 3-D subband samples. Coding results suggest that high-motion video sequences can be encoded at significantly lower rates than those achievable with conventional hybrid coders. Main advantages are the high energy compaction capability and the nonrecursive decoder structure. In the conclusion, the scheme is interpreted more generally, viewed as a motion-compensated short-time spectral analysis of video sequences, which can adapt to the quickness of changes. Although a 3-D multiresolution representation of the picture information is produced, a true multiresolution representation of motion information, based on spatio-temporal decimation and interpolation of the MVF, is regarded as the still-missing part.
Two cell lines from bone marrow that differ in terms of collagen synthesis, osteogenic characteristics, and matrix mineralization. (1993)

Two cloned cell lines were isolated from cultures of mouse bone-marrow cells. One of the lines, D1, exhibited osteogenic properties and synthesized type-I collagen (alpha 1)2 alpha 2. The second cell line, D2, was not osteogenic and produced a collagen homotrimer (alpha 1)3. Whereas the extracellular matrix of the D1 cell cultures contained striated collagen fibrils, presumably composed of type-I collagen, the homotrimer-producing D2 cells did not demonstrate striated collagen fibrils. Instead, they had thin filaments without detectable striations. Sodium ascorbate stimulated collagen synthesis at the transcriptional level in both the D1 and the D2 cells. The bone-producing characteristics of D1 in vitro included high levels of alkaline phosphatase, increased cyclic adenosine monophosphate on treatment with parathyroid hormone, and expression of osteocalcin mRNA. The D1 cells, unlike the D2 cells, produced a mineralized matrix in vitro. Mineralization in the cultures of the D1 cells occurred in nodules of increased cell density, which also contained the cells with the highest concentrations of collagen mRNA, as shown by in situ hybridization. When the D1 cells were implanted in a diffusion chamber in vivo, a mixture of both osteogenic and adipogenic tissues was formed. This indicates that the D1 cell line is derived from an early marrow stromal precursor that is multipotential.
Encephalitogenicity of myelin‐associated oligodendrocytic basic protein and 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase for BALB/c and SJL mice (1998)

In search of new encephalitogenic myelin antigens, the 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and 19 000 MW isoform of myelin-associated oligodendrocytic basic protein (MOBP) were obtained as recombinant proteins by the baculovirus expression system in Spodoptera frugiperda cells and purified to homogeneity by immobilized metal chelate affinity chromatography (IMAC). The purified MOBP was soluble in water and showed retarded migration on sodium dodecyl sulphate-polyacrylamide gel electrophoresis similar to myelin basic protein (MBP). MOBP induced experimental autoimmune encephalomyelitis (EAE) in nine of 15 susceptible SJL OlaHsd mice, causing death in two animals, whereas three of 14 BALB/c mice showed mild symptoms of EAE, manifested as transient weakness of hind limbs. In both mouse strains, periventricular infiltrates of mononuclear cells were observed. In addition, both 46 000 MW and 48 000 MW CNP isoforms were shown to be non-encephalitogenic for both mouse strains.
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids (1997)

The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.
Lymphedema and Quality of Life in Survivors of Early-Stage Breast Cancer (2002)

Lymphedema occurs at appreciable rates, and its impact on long-term quality of life in survivors of early-stage breast cancer should not be underestimated.
Substrate turnover and inhibitor binding as selection parameters in directed evolution of blood coagulation factor Xa (2003)

A library of blood coagulation factor Xa (FXa)-trypsin hybrid proteases was generated and displayed on phage for selection of derivatives with the domain "architecture" of trypsin and the specificity of FXa. Selection based on binding to soybean trypsin inhibitor only provided enzymatically inactive derivatives, due to a specific mutation of serine 195 of the catalytic triad to a glycine, revealing a significant selection pressure for proteolytic inactive derivatives. By including a FXa peptide substrate in the selection mixture, the majority of the clones had retained serine at position 195 and were enzymatically active after selection. Further, with the inclusion of bovine pancreatic trypsin inhibitor, in addition to the peptide substrate, the selected clones also retained FXa specificity after selection. This demonstrates that affinity selection combined with appropriate deselection provides a simple strategy for selection of enzyme derivatives that catalyse a specific reaction.
Experiments with the Chemostat on Spontaneous Mutations of Bacteria (1950)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
DIFFRACTIVE BEAUTY (1998)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Joseph N. Mait, "DIFFRACTIVE BEAUTY," Optics & Photonics News 9(11), 21- (1998) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Column Liquid Chromatography:  Equipment and Instrumentation (2000)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTColumn Liquid Chromatography: Equipment and InstrumentationWilliam R. LaCourseView Author Information Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, Maryland 21250 Cite this: Anal. Chem. 2000, 72, 12, 37–52Publication Date (Web):April 25, 2000Publication History Published online25 April 2000Published inissue 1 June 2000https://pubs.acs.org/doi/10.1021/a1000007ohttps://doi.org/10.1021/a1000007oreview-articleACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1098Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Chromatography,Electrodes,High-performance liquid chromatography,Interfaces,Silica Get e-Alerts