gebhart/patrepeval huggingface dataset:
Construction of Porous Solids from Hydrogen-Bonded Metal Complexes of 1,3,5-Benzenetricarboxylic Acid (1996)

The reaction of M(II) acetate hydrate (M = Co, Ni, and Zn) with 1,3,5-benzenetricarboxylic (BTC) acid yields a material formulated as M3(BTC)2·12H2O. These compounds are isostructural as revealed by their XRPD patterns and a single crystal structure analysis performed on the cobalt containing solid [monoclinic, space group C2, a = 17.482 (6) Å, b = 12.963 (5) Å, c = 6.559 (2) Å, β = 112.04°, V = 1377.8 (8) Å, Z = 4]. This solid is composed of zigzag chains of tetra-aqua cobalt(II) benzenetricarboxylate that are hydrogen-bonded to yield a tightly held 3-D network. Upon liberating 11 water ligands per formula unit a porous solid results, M3(BTC)2·H2O, which was found to reversibly and repeatedly bind water without destruction of the framework. The proposed 1-D channels of the monohydrate have a pore diameter of 4 × 5 Å, which is typical of those observed in zeolites and molecular sieves. The successful inclusion of ammonia into the porous solid was demonstrated. Larger molecules and others without a reactive lone pair are disallowed from entering the channels.
Intact and amino-terminally shortened forms of insulin-like growth factor I induce mammary gland differentiation and development. (1992)

Growth hormone (GH) plays a role in regulating growth and differentiation of immature glandular structures in the mammary gland, but the mechanisms by which the hormone exerts these effects are unknown. We have previously found that GH stimulates insulin-like growth factor I (IGF-I) I mRNA production within the mammary glands of hypophysectomized rats. In this study we set out to determine if IGF-I administration could mimic the action of GH in initiating mammary gland differentiation and development. Two forms of IGF-I, intact and amino-terminally shortened [des-(1-3)-IGF-I], were found to induce the development of terminal end buds and the formation of alveolar structures in the mammary glands of hypophysectomized, castrated, and estradiol-treated sexually immature male rats. The effect of both forms of IGF-I was similar to that obtained with human GH, but the truncated form was at least 5 times more potent than intact IGF-I. These findings suggest that the inductive effect of GH on glandular differentiation is mediated by the GH-induced production of IGF-I or a related molecule within the mammary gland itself.
Isolation of intestinal epithelial cells for the study of differential gene expression along the crypt-villus axis (1991)

Methods for the differential isolation of intestinal epithelial cells from crypt and villus compartments of small intestine have been used in the study of intestinal functions. However, the use of different methods has resulted in discrepant conclusions as to the localization of expressed genes. Therefore, we undertook a careful comparison of two methods of intestinal epithelial cell isolation, the distended intestinal sac method and the everted intestinal sac method. The isolated cell fractions (distended sac fractions 1-10, everted sac fractions 1-5) were evaluated for the expression of two mRNAs whose localization along the crypt-villus axis had been previously elucidated by in situ hybridization: cytochrome P-450IIB1 (expressed in villus cells) and cryptdin (expressed in crypt cells). Northern blots of total or poly(A)+ RNA from each cell population showed that the first fractions from both methods contained P-450IIB1 mRNA, suggesting that both methods allowed the isolation of cells originating from the villus. Cryptdin mRNA was detected only in cell fractions 5-10 using the distended sac method and was not detected in any fractions from the everted sac method. [3H]thymidine incorporation demonstrated that dividing (crypt) cells were successfully removed by the distended sac method, but remained with the everted sac intestinal remnant. Finally, light and electron microscopy of the isolated cells as well as the intestinal remnants confirmed that while undifferentiated crypt cells were present in distended sac cell fractions 9 and 10, they remained with the everted sac remnant. Thus the distended sac protocol was useful for the isolation of cells from tip and crypt compartments.(ABSTRACT TRUNCATED AT 250 WORDS)
Metodologias e recursos disponíveis no ambiente virtual das disciplinas semipresenciais da graduação (2012)

Hodiernamente, com as tecnologias digitais e educacionais, a aplicacao de conceitos, teorias e metodologias mais interativas e dinâmicas estao sendo utilizadas na Educacao a Distância (EAD), mudando o foco do processo de ensinar/aprender, seja em cursos e disciplinas totalmente virtuais seja em cursos e disciplinas semipresenciais, onde o aluno e o centro das atencoes, realizando a auto-aprendizagem. Esta inovacao no ensino superior foi proposta pela Portaria MEC 2.253/01 reformulada pela Portaria MEC 4.059/04 - Brasil, que sugere .a oferta de disciplinas integrantes do curriculo [do ensino superior] que utilizem modalidade semipresencial.. As universidades precisam pesquisar metodologias e utilizar novos recursos tecnologicos com aplicacao didatico-pedagogica que estao a disposicao no Moodle, desenvolvendo formas de ensino nao-convencionais, por meio do lancamento de modalidade de ensino a distância e de atividades teorico praticas. A presente pesquisa nao pretendeu apontar a excelencia na qualidade da EAD nos cursos semipresenciais, mas, sim sugerir alternativas que melhor viabilizem o ambiente virtual como suporte essencial para a construcao do conhecimento do aluno e uma pequena amostra do muito que se tem feito nos ultimos tempos, na expectativa ainda de muitas inovacoes a serem realizadas nesta area que precisam ser mais bem organizadas e sistematizadas. A metodologia da investigacao constituiu-se numa analise qualitativa-descritiva-interpretativa, consubstanciada nos testemunhos de dez professores e vinte e um alunos de diferentes disciplinas dos dois cursos de graduacao pesquisados. Apos a analise das dificuldades significativas encontradas por alunos e professores, sugeriu-se recursos e metodologias didatico-tecnologicos para o desenvolvimento nas disciplinas semipresenciais da graduacao.
Phosphoryl exchange is involved in the mechanism of the insulin receptor kinase (1994)

The cytoplasmic kinase domain of the human insulin receptor (IRKD; M(r) 49 kDa) has been over-expressed in insect cells using the baculovirus expression system. To investigate the kinase mechanism, we have compared the stoichiometry of ADP formation and phosphoryl transfer. After an initial phase of autophosphorylation, ATP is consumed without a stoichiometric increase in incorporated phosphate. During substrate phosphorylation using poly(Glu:Tyr) (4:1) phosphoryl transfer comes close to ATP turnover, which is independent of the presence of the substrate, indicating an increased efficiency (i.e. ATP turnover/phosphate incorporation) of phosphoryl transfer. Autophosphorylation under pulse-chase conditions suggests the existence of a phosphoenzyme intermediate.
Traffic phase effects in packet-switched gateways (1991)

Much of the traffic in existing packet networks is highly periodic, either because of periodic sources (e.g., real time speech or video, rate control) or because window flow control protocols have a periodic cycle equal to the connection roundtrip time (e.g., a network-bandwidth limited TCP bulk data transfer). Control theory suggests that this periodicity can resonate (i.e., have a strong, non-linear interaction) with deterministic estimation or control algorithms in network gateways. 1 In this paper we define the notion of traffic phase in a packet-switched network and describe how phase differences between competing traffic streams can be the dominant factor in relative throughput. Drop Tail gateways in a TCP/IP network with strongly periodic traffic can result in systematic discrimination against some connections. We demonstrate this behavior with both simulations and theoretical analysis. This discrimination can be eliminated with the addition of appropriate randomization to the network. In particular, analysis suggests that simply coding a gateway to drop a random packet from its queue (rather than the tail) on overflow is often sufficient.We do not claim that Random Drop gateways solve all of the problems of Drop Tail gateways. Biases against bursty traffic and long roundtrip time connections are shared by both Drop Tail and Random Drop gateways. Correcting the bursty traffic bias has led us to investigate a different kind of randomized gateway algorithm that operates on the traffic stream, rather than on the queue. Preliminary results show that the Random Early Detection gateway, a newly developed gateway congestion avoidance algorithm, corrects this bias against bursty traffic. The roundtrip time bias (at least in TCP/IP networks) results from the TCP window increase algorithm, not from the gateway dropping policy, and we briefly discuss changes to the window increase algorithm that could eliminate this bias.
Integrated GPS/INS System for Pedestrian Navigation in a Signal Degraded Environment (2006)

systems for pedestrian and vehicular navigation. Dr. Gérard Lachapelle is a Professor of Geomatics Engineering at the University of Calgary where he is responsible for teaching and research related to location, positioning, and navigation. He has been involved with GPS developments and applications since 1980. He has held a Canada Research Chair/iCORE Chair in wireless location since 2001 and heads the PLAN Group at the
Prevention of experimental autoimmune encephalomyelitis and induction of tolerance with acute immunosuppression followed by syngeneic bone marrow transplantation (1992)

Experimental autoimmune encephalomyelitis (EAE) is an inducible autoimmune disease widely used as a model of the acute/relapsing stage of multiple sclerosis. In the present study we examined the effect of acute immunosuppression induced by total body irradiation (TBI) (900 to 1100 centigray (cGy)) or by a single high dose of cyclophosphamide (CY) (300 mg/kg), followed by syngeneic bone marrow transplantation (SBMT), on the development of EAE in SJL/J mice. EAE was induced in SJL/J mice by immunization with spinal cord homogenate in adjuvant. Treatment with TBI (900 cGy) and SBMT on day 6 postimmunization caused a delayed onset and a marked reduction in the incidence and severity of EAE. A higher dose of irradiation (1100 cGy) or the administration of CY followed by SBMT completely abrogated the development of paralysis. None of the 21 mice treated with CY and SBMT, and only 1 of 7 mice treated with TBI (1100 cGy) and SBMT developed clinical signs of EAE during a period of 3 months. Furthermore, mice treated with CY and SBMT became resistant to rechallenge with the same encephalitogenic inoculum. In addition, the lymphocytes obtained from these mice did not proliferate in vitro in response to myelin basic protein or tuberculin-purified protein derivative, unlike lymphocytes from immunized but untreated animals. This absence of reactivity was not associated with alterations in the proportion of the L3T4 and Lyt-2 T-cell subsets nor with a loss in T cell competence as evidenced by the full response of lymphocytes to the T cell mitogen Con A and to a nonrelevant Ag (OVA). Our results indicate that the elimination of effector lymphocytes either by myeloablative doses of CY or ionizing irradiation followed by rescue with SBMT inhibits the development of the autoimmune process in EAE and leads to induction of tolerance to the immunizing Ag by newly developing lymphocytes. This approach of combining immunoablation and reconstitution with autologous bone marrow transplantation may be applicable in the treatment of life-threatening neurologic autoimmune diseases.
Low ringing I/O buffer design (2002)

This paper describes the design of a new I/O buffer architecture, allowing low ringing at the presence of inductive package noise. A simple and accurate model of the I/O buffer can be used to help semi-custom users foresee PAD behavior under different load and inductive packaging situations. Analysis results and simulations of the behavioral model were validated by a discrete prototype. The new buffer design reached higher frequencies without noise degradation when compared with traditional buffer design.
Inhibition of calcineurin by cyclosporin A‐cyclophilin requires calcineurin B (1992)

The interaction of the immunosuppressive complex cyclosporin A-cyclophilin (CsA-CyP) with the Ca2+/calmodulin-dependent protein phosphatase calcineurin is investigated using a recombinant form of the A subunit of calcineurin (rCNA). Only in the presence of purified calcineurin B (CNB) does rCNA show the response of native calcineurin, i.e. 50% inhibition of rCNA phosphatase activity at 6 nM human cyclophilin B and 0.6 microM human cyclophilin A using [32P]casein as substrate, yet stimulation of activity with p-nitrophenyl phosphate as substrate. This study demonstrates that the B subunit is necessary to confer sensitivity of calcineurin to CsA-CyP.
Targeted exon skipping with AAV-mediated split adenine base editors (2019)

Techniques for exclusion of exons from mature transcripts have been applied as gene therapies for treating many different diseases. Since exon skipping has been traditionally accomplished using technologies that have a transient effect, it is particularly important to develop new techniques that enable permanent exon skipping. We have recently shown that this can be accomplished using cytidine base editors for permanently disabling the splice acceptor of target exons. We now demonstrate the application of CRISPR-Cas9 adenine deaminase base editors to disrupt the conserved adenine within splice acceptor sites for programmable exon skipping. We also demonstrate that by altering the amino acid sequence of the linker between the adenosine deaminase domain and the Cas9-nickase or by coupling the adenine base editor with a uracil glycosylase inhibitor, the DNA editing efficiency and exon-skipping rates improve significantly. Finally, we developed a split base editor architecture compatible with adeno-associated viral packaging. Collectively, these results represent significant progress toward permanent in vivo exon skipping through base editing and, ultimately, a new modality of gene therapy for the treatment of genetic diseases.
A digital neuron-type processor and its VLSI design (1989)

A set of neuron-type circuits elements based on logic gate circuits with multi-input multifan output capability is described. Three types of elements are introduced, one called the cell body with its dendritic inputs and synaptic junction, another representing the axon base, and the axon circuit. These three elements are cascaded to form a neuron-type processing element. The circuit performs input temporal and spatial summation as well as thresholding. The entire neuron circuit is simulated and a design is given using VSLI techniques.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Whole genome sequence-enabled prediction of sequences performed for random PCR products of Escherichia coli (2000)

The sequence of an unknown PCR product generated by random (and conventional) PCR could be determined without sequencing when it is provided with the template DNA sequence. Theoretically, this was based on formerly established ideas which assert that the amount of random PCR product mainly depends on the stability of the primer-binding structures and that the dynamic solution structure of DNA is essentially governed by the Watson-Crick base pairing. However, it has not been clear whether this holds true for larger genomes of mega- to gigabase size, beside the lambda phage genome (of 50 kb) used previously, nor has it been ascertained to uniquely specify the sequence of a random PCR product. Here, we jointly use two computer programs together with experimental data from Genome Profiling (i.e. TGGE analysis of random PCR products). The first procedure carried out by a newly remodeled computer program (PCRAna-A1) was shown to be competent to calculate a set of random PCR products from Escherichia coli genome DNA (4.7 Mb). The other procedure performed with another program (Poland-H) played a critical role in determining the final candidate sequence by theoretically offering the initial melting temperature and the melting pattern of unspecified candidate sequences. The success attained here not only proved our method to be useful for sequence prediction but also confirmed the above-mentioned ideas as rational. We believe that this is the first case to computer-utilize a genome sequence as a whole.
Antisense transcription of the ftsZ-ftsA gene junction inhibits cell division in Escherichia coli (1993)

A 490-bp DNA segment spanning the junction between the ftsA and ftsZ genes inhibits cell division when present in high copy number. We show that this segment contains an antisense promoter and an antisense transcription terminator which define a new gene, stfZ.
Dimethylamino‐Pyrazoles and Pyrazolylium Salts (1985)

The cation in the pyrazolylium salt 1 can be regarded as a novel stable ring system with 4π electrons and thus–formally–as antiaromatic. It was prepared from the corresponding 1-unsubstituted pyrazole by oxidation with NO⊕BF.
Molecular analysis of hemolytic and phospholipase C activities of Pseudomonas cepacia (1990)

By using a gene-specific fragment from the hemolytic phospholipase C (PLC) gene of Pseudomonas aeruginosa as a probe and data from Southern hybridizations under reduced stringency conditions, we cloned a 4.2-kb restriction fragment from a beta-hemolytic Pseudomonas cepacia strain which expressed hemolytic and PLC activities in Escherichia coli under the control of the lac promoter. It was found, by using a T7 phage promoter-directed expression system, that this DNA fragment carries at least two genes. One gene which shares significant DNA homology with both PLC genes from P. aeruginosa encodes a 72-kDa protein, while the other gene encodes a 22-kDa protein. When both genes on the 4.2-kb fragment were expressed from the T7 promoter in the same cell, hemolytic and PLC activities could be detected in the cell lysate. In contrast, when each individual gene was expressed in different cells or when lysates containing the translated products of each separate gene were mixed, neither hemolytic activity nor PLC activity could be detected. Clinical and environmental isolates of P. cepacia were examined for beta-hemolytic activity, PLC activity, sphingomyelinase activity, and reactivity in Southern hybridizations with a probe from P. cepacia which is specific for the larger gene which encodes the 72-kDa protein. There were considerable differences in the ability of the different strains to express hemolytic and PLC activities, and the results of Southern DNA-DNA hybridizations of the genomic DNAs of these strains revealed considerable differences in the probe-reactive fragments between high- and medium-stringency conditions as well as remarkable variation in size and number of probe-reactive fragments among different strains. Analysis of the genomic DNAs from hemolytic and nonhemolytic variants of an individual strain (PC-69) by agarose gel electrophoresis. Southern hybridization, and transverse alternating pulsed field gel electrophoresis suggests that the conversion of the hemolytic phenotype to the nonhemolytic phenotype is associated with either the loss of a large plasmid (greater than 200 kb) or a large deletion of the chromosome of P. cepacia PC-69.
Functional Overlap of Finger Representations in Human SI and SII Cortices (2001)

We aimed to find out to what extent functional representations of different fingers of the two hands overlap at the human primary and secondary somatosensory cortices SI and SII. Somatosensory evoked fields (SEFs) were recorded with a 306-channel neuromagnetometer from 8 subjects. Tactile stimuli, produced by diaphragms driven by compressed air, were delivered to the fingertips in three different conditions. First, the right index finger was stimulated once every 2 s. Then two other stimuli were interspersed, in different sessions, to right- or left-hand fingers (thumb, middle finger, or ring finger) between the successive right index finger stimuli. Strengths of the responses to right index finger stimuli were evaluated in each condition. Responses to right index finger stimuli were modeled by three current dipoles, located at the contralateral SI and the SII cortices of both hemispheres. The earliest SI responses, peaking around 65 ms, were suppressed by 18% (P < 0.05) when the intervening stimuli were presented to the same hand; intervening stimuli to the other hand had no effect. The SII responses were bilaterally suppressed by intervening stimuli presented to either hand: in the left SII, the suppression was 39 and 42% (P < 0.01) and in the right SII 67 and 72% (P < 0.001) during left- and right-sided intervening stimuli, respectively. Left- and right-sided intervening stimuli affected similarly the SII responses and had no effect on the response latencies. The results indicate a strong and symmetric overlap of finger representations for both hands in the human SII cortices, and a weaker functional overlap for fingers of the same hand in the SI cortex.
Palladium-Catalyzed Amination of Aryl Triflates (1997)

The conversion of aryl triflates to the corresponding aniline derivatives was accomplished in moderate to good yield using a catalyst consisting of the combination of palladium acetate (2 mol %) and either BINAP or Tol-BINAP. In contrast to the corresponding palladium-catalyzed amination of aryl bromides and iodides, electronically neutral aryl triflates gave higher yields of arylamines than did electron deficient aryl triflates, presumably due to the increased rate of base-promoted triflate cleavage in electron deficient substrates.
Neutralizing Antibodies Protect against Lethal Flavivirus Challenge but Allow for the Development of Active Humoral Immunity to a Nonstructural Virus Protein (1998)

Antibody-mediated neutralization of viruses has been extensively studied in vitro, but the precise mechanisms that account for antibody-mediated protection against viral infection in vivo still remain largely uncharacterized. The two points under discussion are antibodies conferring sterilizing immunity by neutralizing the virus inoculum or protection against the development of disease without complete inhibition of virus replication. For tick-borne encephalitis virus (TBEV), a flavivirus, transfer of neutralizing antibodies specific for envelope glycoprotein E protected mice from subsequent TBEV challenge. Nevertheless, short-term, low-level virus replication was detected in these mice. Furthermore, mice that were exposed to replicating but not to inactivated virus while passively protected developed active immunity to TBEV rechallenge. Despite the priming of TBEV-specific cytotoxic T cells, adoptive transfer of serum but not of T cells conferred immunity upon naive recipient mice. These transferred sera were not neutralizing and were predominantly specific for NS1, a nonstructural TBEV protein which is expressed in and on infected cells and which is also secreted from these cells. Results of these experiments showed that despite passive protection by neutralizing antibodies, limited virus replication occurs, indicating protection from disease rather than sterilizing immunity. The protective immunity induced by replicating virus is surprisingly not T-cell mediated but is due to antibodies against a nonstructural virus protein absent from the virion.