1623 A FLUORESCENT IMAGING AGENT SPECIFICALLY TARGET PROSTATE SPECIFIC MEMBRANE ANTIGEN-EXPRESSING CELLS (2011)

You have accessJournal of UrologyProstate Cancer: Basic Research1 Apr 20111623 A FLUORESCENT IMAGING AGENT SPECIFICALLY TARGET PROSTATE SPECIFIC MEMBRANE ANTIGEN-EXPRESSING CELLS John Eifler, Wasim Chowdhury, Catherine Foss, Mark Castanares, Ying Chen, Netto George, Martin Pomper, and Ronald Rodriguez John EiflerJohn Eifler Baltimore, MD More articles by this author , Wasim ChowdhuryWasim Chowdhury Baltimore, MD More articles by this author , Catherine FossCatherine Foss Baltimore, MD More articles by this author , Mark CastanaresMark Castanares Baltimore, MD More articles by this author , Ying ChenYing Chen Baltimore, MD More articles by this author , Netto GeorgeNetto George Baltimore, MD More articles by this author , Martin PomperMartin Pomper Baltimore, MD More articles by this author , and Ronald RodriguezRonald Rodriguez Baltimore, MD More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1731AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES A urea-based small molecule conjugated to a near-infrared fluorophore (YC-27)has been developed at our institution which targets the prostate specific membrane antigen (PSMA). Such an agent may be suitable for real-time fluorescence-based surgery for detection of positive surgical margins and micrometastatic disease intraoperatively. We sought to determine the sensitivity of this compound in vitro. METHODS A PSMA-expressing prostate cancer cell line and a non-PSMA-expressing prostate cancer cell line were incubated with 10nM YC-27 at 37° C for 30 minutes. The cells were then washed twice with phosphate buffered saline (PBS). 1 million cells were added to each well in a 96-well plate, with a different percentage of PSMA-positive cells in each well, to determine the minimum number of cells that may be differentiated from background. The cells were then imaged using an optical imaging instrument, the PEARL Imager (Li-Cor Biosciences). In a separate experiment, 75,000 PC3-PIP, PC3-Control, and PC3 transfected with PSMA were incubated with YC-27, washed, and placed in a 96-well plate. After 24 hours, increasing numbers of unstained PC3 cells were added to the plate. The plate was then centrifuged and the cells were imaged with the PEARL Imager. RESULTS In the first experiment, the PSMA-based compound was detected above background and were visible with the PEARL imager when 10,000 cells were present (Figure 1) and the level of signal displayed a linear relationship. In a separate experiment, it was seen that 75,000 PC3-PIP, PC3 Control, and PC3-PSMA cells formed a confluent layer at 24h. After plating 75,000 stained cells, signal was not diminished by the addition of unstained cells, even up to 10 layers of unstained cells (Figure 2). CONCLUSIONS A fluorescent-labeled urea-based small molecule can target PSMA with high sensitivity and specificity in an in vivo model and fluorescence signal is able to penetrate at least 10 cell layers. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e650-e651 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information John Eifler Baltimore, MD More articles by this author Wasim Chowdhury Baltimore, MD More articles by this author Catherine Foss Baltimore, MD More articles by this author Mark Castanares Baltimore, MD More articles by this author Ying Chen Baltimore, MD More articles by this author Netto George Baltimore, MD More articles by this author Martin Pomper Baltimore, MD More articles by this author Ronald Rodriguez Baltimore, MD More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...