gebhart/patrepeval huggingface dataset:
Physiological changes in respiratory function associated with ageing (1999)

Physiological ageing of the lung is associated with dilatation of alveoli, enlargement of airspaces, decrease in exchange surface area and loss of supporting tissue for peripheral airways ("senile emphysema"), changes resulting in decreased static elastic recoil of the lung and increased residual volume and functional residual capacity. Compliance of the chest wall diminishes, thereby increasing work of breathing when compared with younger subjects. Respiratory muscle strength also decreases with ageing, and is strongly correlated with nutritional status and cardiac index. Expiratory flow rates decrease with a characteristic alteration in the flow-volume curve suggesting small airway disease. The ventilation-perfusion ratio (V'A/Q') heterogeneity increases, with low V'A/Q' zones appearing as a result of premature closing of dependent airways. Carbon monoxide transfer decreases with age, reflecting mainly a loss of surface area. In spite of these changes, the respiratory system remains capable of maintaining adequate gas exchange at rest and during exertion during the entire lifespan, with only a slight decrease in arterial oxygen tension, and no significant change in arterial carbon dioxide tension. Ageing tends to diminish the reserve of the respiratory system in cases of acute disease. Decreased sensitivity of respiratory centres to hypoxia or hypercapnia results in a diminished ventilatory response in cases of heart failure, infection or aggravated airway obstruction. Furthermore, decreased perception bronchoconstriction and diminished physical activity may result in lesser awareness of the disease and delayed diagnosis.
Monoclonal antibodies to human laminin α4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of α6β1 integrin and MCAM to α4-laminins (2014)

α4-Laminins, such as laminins 411 and 421, are mesenchymal laminins expressed by vascular and lymphatic endothelial cells, leukocytes and other normal cell types. These laminins are recognized by α6β1 and α6β4 integrins and MCAM (CD146), and promote adhesion and migration of the cells. α4-Laminins are also expressed and secreted by some tumor cells and strongly promote tumor cell migration. Moreover, the abluminal side of blood and/or lymphatic vessels and the nerve perineurium, common tracks of tumor cell dissemination, express α4-laminins, and these laminin isoforms, when expressed in the stroma, may contribute to tumor invasion. In the present study, we examined ten mAbs to human laminin α4 chain for their reactivity with the isolated laminin α4 globular domain, their ability to inhibit tumor cell adhesion and migration on laminins 411 and 421, and their effect on the binding of α6β1 integrin and MCAM to both α4-laminins. Most of the mAbs reacted with the laminin α4 globular domain, but only two, mAbs FC10 and 084, significantly inhibited tumor cell adhesion and migration on laminin-411. When used in combination, these antibodies practically abolished the cell adhesion and migration on laminin-411 and significantly reduced the cellular responses on laminin-421. Accordingly, mAbs FC10 and 084 significantly inhibited the binding of purified α6β1 integrin and MCAM to laminins 411 and 421. These results indicate that mAbs to the laminin α4 globular domain are able to inhibit tumor cell adhesion and migration on laminins 411 and 421, and that α6β1 integrin and MCAM bind α4-laminins at very close sites on the globular domain. These reagents contribute to a better understanding of the biology of α4-laminins and may have a therapeutic potential in malignant and inflammatory diseases.
Reduced-order models based on linear and nonlinear aerodynamic impulse responses (1999)

This paper discusses a method for the identification and application of reduced-order models based on linear and nonlinear aerodynamic impulse responses. The Volterra theory of nonlinear systems and an appropriate kernel identification technique are described. Insight into the nature of kernels is provided by applying the method to the nonlinear Riccati equation in a non-aerodynamic application. The method is then applied to a nonlinear aerodynamic model of an RAE 2822 supercritical airfoil undergoing plunge motions using the CFL3D Navier-Stokes flow solver with the Spalart-Allmaras turbulence model. Results demonstrate the computational efficiency of the technique.
Adeno-Associated Virus Type 2-Mediated Gene Transfer: Role of Cellular FKBP52 Protein in Transgene Expression (2001)

Although adeno-associated virus type 2 (AAV) has gained attention as a potentially useful vector for human gene therapy, the transduction efficiencies of AAV vectors vary greatly in different cells and tissues in vitro and in vivo. We have documented that a cellular tyrosine phosphoprotein, designated the single-stranded D-sequence-binding protein (ssD-BP), plays a crucial role in AAV-mediated transgene expression (K. Y. Qing, X.-S. Wang, D. M. Kube, S. Ponnazhagan, A. Bajpai, and A. Srivastava, Proc. Natl. Acad. Sci. USA 94:10879-10884, 1997). We have documented a strong correlation between the phosphorylation state of ssD-BP and AAV transduction efficiency in vitro as well as in vivo (K. Y. Qing, B. Khuntrirat, C. Mah, D. M. Kube, X.-S. Wang, S. Ponnazhagan, S. Z. Zhou, V. J. Dwarki, M. C. Yoder, and A. Srivastava, J. Virol. 72:1593-1599, 1998). We have also established that the ssD-BP is phosphorylated by epidermal growth factor receptor protein tyrosine kinase and that the tyrosine-phosphorylated form, but not the dephosphorylated form, of ssD-BP prevents AAV second-strand DNA synthesis and, consequently, results in a significant inhibition of AAV-mediated transgene expression (C. Mah, K. Y. Qing, B. Khuntrirat, S. Ponnazhagan, X.-S. Wang, D. M. Kube, M. C. Yoder, and A. Srivastava, J. Virol. 72:9835-9841, 1998). Here, we report that a partial amino acid sequence of ssD-BP purified from HeLa cells is identical to a portion of a cellular protein that binds the immunosuppressant drug FK506, termed the FK506-binding protein 52 (FKBP52). FKBP52 was purified by using a prokaryotic expression plasmid containing the human cDNA. The purified protein could be phosphorylated at both tyrosine and serine or threonine residues, and only the phosphorylated forms of FKBP52 were shown to interact with the AAV single-stranded D-sequence probe. Furthermore, in in vitro DNA replication assays, tyrosine-phosphorylated FKBP52 inhibited AAV second-strand DNA synthesis by greater than 90%. Serine- or threonine-phosphorylated FKBP52 caused approximately 40% inhibition, whereas dephosphorylated FKBP52 had no effect on AAV second-strand DNA synthesis. Deliberate overexpression of FKBP52 effectively reduced the extent of tyrosine phosphorylation of the protein, resulting in a significant increase in AAV-mediated transgene expression in human and murine cell lines. These studies corroborate the idea that the phosphorylation status of the cellular FKBP52 protein correlates strongly with AAV transduction efficiency, which may have important implications for the optimal use of AAV vectors in human gene therapy.
Advances in HIV/AIDS therapy. (2000)

Advances in HIV/AIDS therapy have been rapid and profound. An improved understanding of HIV pathogenesis has demonstrated the need for aggressive antiretroviral therapy in most persons with HIV infection. Combinations of at least three drugs are necessary to fully suppress viral replication. Suppression of viral replication prevents the development of drug resistance and allows immune reconstitution to occur. Current therapies do have limitations including side effects, cross-resistance, adherence challenges, and drug interactions. However, most patients have derived great benefit from these therapies. The consequences of immune reconstitution have included resolution of existing opportunistic infections and a markedly reduced risk of new infections. In certain settings, immune reconstitution may allow the discontinuation of opportunistic infection prophylaxis or treatments. The net result has been a substantial improvement in the quality and quantity of life for HIV-infected patients.
α-Internexin: A Novel Biomarker for Pancreatic Neuroendocrine Tumor Aggressiveness (2014)

α-Internexin can be a useful prognostic biomarker for PNETs.
HOW DO THEY KNOW THEIR CUSTOMERS SO WELL (2001)

Many firms know about their customers, but few know the customers themselves or how to get new ones. Leaders in customer-knowledge management go beyond transaction data, using a mix of techniques, and they aren't afraid to tackle difficult problems.

Davenport, director of the Institute for Strategic Change, Accenture (formerly Andersen Consulting) and coauthors Harris, also from Accenture, and Kohli, professor of marketing at Emory University, report results from interviews with 24 leading firms and describe seven practices that the leaders share. The companies interviewed ? including Harley-Davidson, Procter & Gamble, and Wachovia Bank ? have undertaken specific and successful initiatives centered around the management of customer knowledge.

Within the seven practices, two results stand out: First, firms are beginning to rely more on data from actual interactions, such as sales and service. They are learning that customers are more than transactions, and they are seeking creative ways to turn data from these interactions ? human data ? into knowledge. Second, even the most ambitious firms are keeping data from different approaches separate. They are not accepting the notion of an integrated data repository.

Focus on the most valued customers. Know which customers are worth the organization's resources. 

Prioritize objectives. Successful firms begin all customer-knowledge management initiatives by prioritizing business strategies and customer-relationship objectives. They know which customers to focus on and what new behaviors the customers should exhibit.

Aim for the optimal knowledge mix. There's no single solution to knowledge management. Use a variety of approaches. 

Don't use one repository for all data. The fully integrated customer-knowledge environment seems more an intriguing idea than a practical reality. Diverse forms of information are difficult to combine in one set of database records, and firms risk having a departing employee walk away with highly developed knowledge. Consequently, customer data is fragmented across multiple systems and locations. No one has been able to combine hard (transaction-based) and soft knowledge in one customer database.

Think creatively about human knowledge. This is the main practice that separates the leaders from the laggards. We saw many creative solutions to managing both explicit (documented and accessible) and tacit (understood but undocumented and not accessible) knowledge.

Look at the broader context. Customer-knowledge initiatives do not exist in a vacuum. Their success depends on the organization's roles and responsibilities, the workplace culture, and the organizational structure.

Establish a process and tools. Many firms seem to stop working when they've selected a management strategy ? avoiding the planning that is critical to implementation. The leading firms work hard to deliberately manage customer knowledge, using a defined process and creating tools as needed.
Principles of Design of Superhydrophobic Coatings by Deposition from Dispersions (2009)

The analysis demonstrating the abilities of various types of ordered textures, constructed from spherical particles, to provide high water contact angles on the surface of the hydrophobic material is presented. For several ordered three-dimensional surface structures we have derived the equations, relating the contact angles, formed by water droplets on a textured coating, with the geometric parameters of the texture and the chemical properties of the coating. The conditions providing the thermodynamic stability of heterogeneous wetting regime on textured surfaces have been discussed. It was demonstrated that multimodal surface roughness facilitates essentially the attainment of the superhydrophobic state and the surface textures exhibiting the stable superhydrophobic properties may be effectively manufactured by controlled aggregation of particles. Based on the analysis of forces acting between the nanoparticles both in the bulk dispersions and in the deposited film, we discuss the correlation of the properties of dispersion with the surface textures formed.
Vehicle Fingerprinting for Reacquisition and Tracking in Videos (2005)

Visual recognition of objects through multiple observations is an important component of object tracking. We address the problem of vehicle matching when multiple observations of a vehicle are separated in time such that frames of observations are not contiguous, thus prohibiting the use of standard frame-to-frame data association. We employ features extracted over a sequence during one time interval as a vehicle fingerprint that is used to compute the likelihood that two or more sequence observations are from the same or different vehicles. The challenges of change in pose, aspect and appearances across two disparate observations are handled by combining feature-based quasi-rigid alignment with flexible matching between two or more sequences. The current work uses the domain of vehicle tracking from aerial platforms where typically both the imaging platform and the vehicles are moving and the number of pixels on the object are limited to fairly low resolutions. Extensive evaluation with respect to ground truth is reported in the paper.
Optimised ligation of oligonucleotides by thermal ligases: comparison of Thermus scotoductus and Rhodothermus marinus DNA ligases to other thermophilic ligases (2000)

We describe the characterisation of four thermo-stable NAD(+)-dependent DNA ligases, from Thermus thermophilus (Tth), Thermus scotoductus (Ts), Rhodothermus marinus (Rm) and Thermus aquaticus (Taq), by an assay which measures ligation rate and mismatch discrimination. Complete libraries of octa-, nona- and decanucleotides were used as substrates. The assay comprised the polymerisation of oligo-nucleotides initiated from a 17 base 'primer', using M13mp18 ssDNA as template. Polymers of ligation products were analysed by polyacrylamide gel electro-phoresis. Under optimum conditions, the enzymes produced polymers ranging from 8 to 16 additions; there was variation between enzymes and the length of the oligonucleotides had a strong effect. The optimal total oligonucleotide concentration for each library was approximately 4 nmol. We compared the rates of ligation between the four ligases using an octanucleotide library as substrate. By this criterion, the Ts and Rm ligases are far more active compared to the more commonly available thermostable ligases.
Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses (2005)

Organic osmolytes are small solutes used by cells of numerous water-stressed organisms and tissues to maintain cell volume. Similar compounds are accumulated by some organisms in anhydrobiotic, thermal and possibly pressure stresses. These solutes are amino acids and derivatives, polyols and sugars, methylamines, methylsulfonium compounds and urea. Except for urea, they are often called ;compatible solutes', a term indicating lack of perturbing effects on cellular macromolecules and implying interchangeability. However, these features may not always exist, for three reasons. First, some of these solutes may have unique protective metabolic roles, such as acting as antioxidants (e.g. polyols, taurine, hypotaurine), providing redox balance (e.g. glycerol) and detoxifying sulfide (hypotaurine in animals at hydrothermal vents and seeps). Second, some of these solutes stabilize macromolecules and counteract perturbants in non-interchangeable ways. Methylamines [e.g. trimethylamine N-oxide (TMAO)] can enhance protein folding and ligand binding and counteract perturbations by urea (e.g. in elasmobranchs and mammalian kidney), inorganic ions, and hydrostatic pressure in deep-sea animals. Trehalose and proline in overwintering insects stabilize membranes at subzero temperatures. Trehalose in insects and yeast, and anionic polyols in microorganisms around hydrothermal vents, can protect proteins from denaturation by high temperatures. Third, stabilizing solutes appear to be used in nature only to counteract perturbants of macromolecules, perhaps because stabilization is detrimental in the absence of perturbation. Some of these solutes have applications in biotechnology, agriculture and medicine, including in vitro rescue of the misfolded protein of cystic fibrosis. However, caution is warranted if high levels cause overstabilization of proteins.
Tyrosine Residues Serve as Proton Donor in the Catalytic Mechanism of Epoxide Hydrolase from Agrobacterium radiobacter (2000)

Epoxide hydrolase from Agrobacterium radiobacter catalyzes the hydrolysis of epoxides to their diols via an alkyl-enzyme intermediate. The recently solved X-ray structure of the enzyme shows that two tyrosine residues (Tyr152 and Tyr215) are positioned close to the nucleophile Asp107 in such a way that they can serve as proton donor in the alkylation reaction step. The role of these tyrosines, which are conserved in other epoxide hydrolases, was studied by site-directed mutagenesis. Mutation of Tyr215 to Phe and Ala and mutation of Tyr152 to Phe resulted in mutant enzymes of which the k(cat) values were only 2-4-fold lower than for wild-type enzyme, whereas the K(m) values for the (R)-enantiomers of styrene oxide and p-nitrostyrene oxide were 3 orders of magnitude higher than the K(m) values of wild-type enzyme, showing that the alkylation half-reaction is severely affected by the mutations. Pre-steady-state analysis of the conversion of (R)-styrene oxide by the Y215F and Y215A mutants showed that the 1000-fold elevated K(m) values were mainly caused by a 15-40-fold increase in K(S) and a 20-fold reduction in the rate of alkylation. The rates of hydrolysis of the alkyl-enzyme intermediates were not significantly affected by the mutations. The double mutant Y152F+Y215F showed only a low residual activity for (R)-styrene oxide, with a k(cat)/K(m) value that was 6 orders of magnitude lower than with wild-type enzyme and 3 orders of magnitude lower than with the single tyrosine mutants. This indicates that the effects of the mutations were cumulative. The side chain of Gln134 is positioned in the active site of the X-ray structure of epoxide hydrolase. Mutation of Gln134 to Ala resulted in an active enzyme with slightly altered steady-state kinetic parameters compared to wild-type enzyme, indicating that Gln134 is not essential for catalysis and that the side chain of Gln134 mimics bound substrate. Based upon this observation, the inhibitory potential of various unsubstituted amides was tested, resulting in the identification of phenylacetamide as a competitive inhibitor with an inhibition constant of 30 microM.
Stabilization of the p53 tumor suppressor is induced by adenovirus 5 E1A and accompanies apoptosis. (1993)

Oncogenic transformation by human adenoviruses requires early regions 1A and 1B (E1A and E1B) and provides a model of multistep carcinogenesis. This study shows that the metabolic stabilization of p53 observed in adenovirus 5 (Ad5)-transformed cells can occur in untransformed cells expressing E1A alone. Stabilized p53 was localized to the nucleus and was indistinguishable from wild-type p53 with respect to its interactions with hsc70, PAb420, Ad5 p55E1B, and SV40 large T antigen. Moreover, binding of Ad5 p55E1B or SV40 large T antigen had no additional effect on p53 levels or turnover. Higher levels of p53 were also induced in a variety of cell types within 40 hr after transferring E1A genes. E1A also caused cells to lose viability by a process resembling apoptosis. The apoptosis appeared to involve p53, because p53 levels reverted to normal in surviving cells that had lost E1A, and E1B protected cells from the toxic effects of E1A. These results suggest that (1) the involvement of p53 in tumor suppression and/or apoptosis can be regulated at the level of protein turnover, and (2) a major oncogenic role for E1B is to counter cellular responses to E1A (i.e., stabilization of p53 and associated apoptosis) that preclude transformation by E1A alone. This represents the first physiological setting in which high levels of endogenous p53 are induced in response to an oncogenic challenge, with the apparent consequence of suppressing transformation.
Trimetrexate in Relapsed T-Cell Lymphoma With Skin Involvement (2002)

TMTX is active with acceptable toxicity in this population and merits further investigation.
Network-Centric Quantum Communications with Application to Critical Infrastructure Protection (2013)

Network-centric quantum communications (NQC) - a new, scalable instantiation of quantum cryptography providing key management with forward security for lightweight encryption, authentication and digital signatures in optical networks - is briefly described. Results from a multi-node experimental test-bed utilizing integrated photonics quantum communications components, known as QKarDs, include: quantum identification; verifiable quantum secret sharing; multi-party authenticated key establishment, including group keying; and single-fiber quantum-secured communications that can be applied as a security retrofit/upgrade to existing optical fiber installations. A demonstration that NQC meets the challenging simultaneous latency and security requirements of electric grid control communications, which cannot be met without compromises using conventional cryptography, is described.
Differential Myocardial Infarct Repair with Muscle Stem Cells Compared to Myoblasts (2005)

Myoblast transplantation for cardiac repair has generated beneficial results in both animals and humans; however, poor viability and poor engraftment of myoblasts after implantation in vivo limit their regeneration capacity. We and others have identified and isolated a subpopulation of skeletal muscle-derived stem cells (MDSCs) that regenerate skeletal muscle more effectively than myoblasts. Here we report that in comparison with a myoblast population, MDSCs implanted into infarcted hearts displayed greater and more persistent engraftment, induced more neoangiogenesis through graft expression of vascular endothelial growth factor, prevented cardiac remodeling, and elicited significant improvements in cardiac function. MDSCs also exhibited a greater ability to resist oxidative stress-induced apoptosis compared to myoblasts, which may partially explain the improved engraftment of MDSCs. These findings indicate that MDSCs constitute an alternative to other myogenic cells for use in cardiac repair applications.
A Card Trick Mnemonic Revisited (1994)

In the November 1968 and the May 1977 Word Ways, I described the use of the word BIBLE ATLAS GOOSE THIGH as a mnemonic for performing a bit of card-reading "magic". Twenty playing cards are dealt face down in ten pairs and, while the magician's back is turned, the subject looks at and memorizes the cards of one pair. The twenty cards are then gathered into one pile, preserving the pairs, and dealt face up in a four-by-five array according to the mnemonic: the first two cards in the first and third positions of the first row (the two B's in BIBLE), the next two cards in the second position of the first row and the third position of the fourth row (the two I's in BIBLE and THIGH), etc.
Accelerating SQL database operations on a GPU with CUDA (2010)

Prior work has shown dramatic acceleration for various database operations on GPUs, but only using primitives that are not part of conventional database languages such as SQL. This paper implements a subset of the SQLite command processor directly on the GPU. This dramatically reduces the effort required to achieve GPU acceleration by avoiding the need for database programmers to use new programming languages such as CUDA or modify their programs to use non-SQL libraries.