gebhart/patrepeval huggingface dataset:
Multifunctional Peptide Synthetases (1997)

ADVERTISEMENT RETURN TO ISSUEPREVArticleMultifunctional Peptide SynthetasesHans von Döhren, Ullrich Keller, Joachim Vater, and Rainer ZocherView Author Information Section Biochemistry and Molecular Biology, Max-Volmer-Institute of Biophysical Chemistry and Biochemistry, Technical University Berlin, Franklinstrasse 29, D-10587 Berlin, Germany Cite this: Chem. Rev. 1997, 97, 7, 2675–2706Publication Date (Web):November 10, 1997Publication History Received9 June 1997Revised26 September 1997Published online10 November 1997Published inissue 1 November 1997https://pubs.acs.org/doi/10.1021/cr9600262https://doi.org/10.1021/cr9600262research-articleACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views1514Altmetric-Citations197LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biosynthesis,Condensation,Lactones,Monomers,Peptides and proteins Get e-Alerts
β1C Integrin in Epithelial Cells Correlates with a Nonproliferative Phenotype (1998)

The expression of the beta1C integrin, an alternatively spliced variant of the beta1 subunit, was investigated in human adult and fetal tissues. In the adult, beta1C immunoreactivity was found in nonproliferative, differentiated simple, and/or pseudostratified epithelia in prostate glands and liver bile ducts. In contrast, beta1C was undetectable in stratified squamous epithelium of the epidermis and/or in hepatocytes. Luminal prostate epithelial cells expressed beta1C in vivo and in vitro, but no beta1C was seen in basal cells, which are proliferating cells. Fetal prostate expressed beta1C in differentiated glands that had a defined lumen, but not in budding glands, indicating that beta1C is a marker of prostate epithelium differentiation. The beta1C and the common beta1A variants are differentially distributed: beta1A was found in luminal and basal epithelial as well as in stromal cells in the prostate. In the liver, beta1C and beta1A were coexpressed in biliary epithelium, whereas vascular cells expressed only beta1A. Because we found beta1C in nonproliferative and differentiated epithelium, we investigated whether beta1C could have a causal role in inhibiting epithelial cell proliferation. The results showed that exogenous expression of a beta1C, but not of a beta1A, cytoplasmic domain chimeric construct, completely inhibited thymidine incorporation in response to serum by prostate cancer epithelial cells. Consistent with these in vitro results, beta1C appeared to be downregulated in prostate glands that exhibit regenerative features in benign hyperplastic epithelium. These data show that the presence of beta1C integrins in epithelial cells correlates with a nonproliferative, differentiated phenotype and is growth inhibitory to prostate epithelial cells in vitro. These findings indicate a novel pathophysiological role for this integrin variant in epithelial cell proliferation.
Giant strongly connected component of directed networks (2001)

We describe how to calculate the sizes of all giant connected components of a directed graph, including the strongly connected one. In particular, the World Wide Web is a directed network. The results are obtained for graphs with statistically uncorrelated vertices and an arbitrary joint in and out-degree distribution P(k(i),k(o)). We show that if P(k(i),k(o)) does not factorize, the relative size of the giant strongly connected component deviates from the product of the relative sizes of the giant in- and out-components. The calculations of the relative sizes of all the giant components are demonstrated using the simplest examples. We explain that the giant strongly connected component may be less resilient to random damage than the giant weakly connected one.
Therapeutic potential of matrix metalloprotease inhibitors in neuropathic pain (2010)

Targeted therapy using specific MMP inhibitors, siRNAs, peptide inhibitors and monoclonal antibodies can provide a better way of treatment by blocking a single MMP and can reduce the side effects of broad-spectrum MMP inhibitors.
Preventing Discrimination Against Volunteers in Prophylactic HIV Vaccine Trials: Lessons From a Phase II Trial (1998)

In this vaccine trial, few serious social harms were reported. Those who conduct HIV tests for insurance, employment, health care, or other reasons should be made aware that HIV vaccines can cause false-positive HIV test results. Those planning future trials must continue to provide needed support to volunteers. Social harms should be monitored with the same vigilance accorded to physical harms.
Targeted Expression of Major Histocompatibility Complex (MHC) Class II Molecules Demonstrates that Dendritic Cells Can Induce Negative but Not Positive Selection of Thymocytes In Vivo (1997)

It is well established that lymphoid dendritic cells (DC) play an important role in the immune system. Beside their role as potent inducers of primary T cell responses, DC seem to play a crucial part as major histocompatibility complex (MHC) class II+ "interdigitating cells" in the thymus during thymocyte development. Thymic DC have been implicated in tolerance induction and also by some authors in inducing major histocompatibility complex restriction of thymocytes. Most of our knowledge about thymic DC was obtained using highly invasive and manipulatory experimental protocols such as thymus reaggregation cultures, suspension cultures, thymus grafting, and bone marrow reconstitution experiments. The DC used in those studies had to go through extensive isolation procedures or were cultured with recombinant growth factors. Since the functions of DC after these in vitro manipulations have been reported to be not identical to those of DC in vivo, we intended to establish a system that would allow us to investigate DC function avoiding artificial interferences due to handling. Here we present a transgenic mouse model in which we targeted gene expression specifically to DC. Using the CD 11c promoter we expressed MHC class II I-E molecules specifically on DC of all tissues, but not on other cell types. We report that I-E expression on thymic DC is sufficient to negatively select I-E reactive CD4+ T cells, and to a less complete extent, CD8+ T cells. In contrast, it only DC expressed I-E in a class II-deficient background, positive selection of CD4+ T cells could not be observed. Thus negative, but not positive, selection events can be induced by DC in vivo.
Research on the Advertisement Effect of Push Type Mobile Advertisement (2009)

Due to the development in mobile telecommunication, mobile advertisements using mobile telecommunication devices are drawing the attention as the new advertisement mean, following the internet advertisement, and the market size of the mobile advertisement is forecasted to only grow. Understanding of the characteristic of mobile medium and analyses of and application plans to the various newly developed advertisement types are necessary for the effective mobile advertisement. In this research, advertisement effects of the push type advertisement, among the mobile advertisement types, on the users were empirically analyzed. The push type advertisement is a marketing method that forcibly exposes the advertisements to the users, and the main examples are location based type, time based type, wireless internet connection type, multimedia type and coupon type advertisements. Advertisements were created based on the above five types, and a survey was carried out with the preselected surveyees as the subject. The result analysis was researched by dividing them into buying action effect and reminiscence effect based on the types. As the result, the differences in the advertisement effect by push type mobile advertisements was found to exist, and the effects on multimedia type and coupon type advertisement were especially large. In the future, research, not just on the push type advertisement, but also on the pull type advertisement should be carried out. And the research to eliminate the negative perceptions on mobile advertisements is necessary to increase the effect of mobile advertisements.
[The presence of antithiamine factor--thiaminase--in organs of random-bred albino rats and mice]. (1991)

Thiaminase activity was not found in rat and mice tissues (liver, kidney tissues) using radiometric procedure. Intact exogenous thiaminase I from bivalve freshwater mollusks was effectively detected in homogenates of rat or mice liver and kidney tissues within 6 hrs after the enzyme parenteral administration into these animals; at the same time, considerable amount of 14C-thiazol developed after thiaminase catalysis was estimated in urine.
Molecular Mechanisms of Vascular Permeability in Diabetic Retinopathy (1999)

Diabetes leads to a wide array of complications in humans, including kidney failure, vascular disease, peripheral nerve degeneration, and vision loss. Diabetic retinopathy causes blindness in more working-age people in the United States than any other disease and contributes greatly to blindness in the young and old as well. The increasing rate of diabetes occurring in our society can only bring about a further decrease in the visual health of this country unless new modalities are discovered to prevent and cure diabetic retinopathy. Breakdown of the blood-retinal barrier and the resultant vascular permeability remains one of the first observable alterations in diabetic retinopathy and strongly correlates with vision loss. In this article, we examine the molecular components that form this blood-retinal barrier and explore how changes in the production of growth factors in the neural parenchyma cause an increase in vascular permeability and contribute to retinal degeneration.
Chronic Myeloid Leukemia Stem Cells (2008)

Although rare, chronic myeloid leukemia (CML) represents an important paradigm for understanding the molecular events leading to malignant transformation of primitive hematopoietic progenitors. CML was the first cancer to be associated with a defined genetic abnormality, BCR-ABL, that is necessary and sufficient for initiating chronic phase disease as well as the first cancer to be treated with molecular targeted therapy. Malignant progenitors or leukemia stem cells (LSCs) evolve as a result of both epigenetic and genetic events that alter hematopoietic progenitor differentiation, proliferation, survival, and self-renewal. LSCs are rare and divide less frequently, and thus, represent a reservoir for relapse and resistance to a molecularly targeted single agent. On subverting developmental processes normally responsible for maintaining robust life-long hematopoiesis, the LSCs are able to evade the majority of current cancer treatments that target rapidly dividing cells. Enthusiasm for the enormous success of tyrosine kinase inhibitors at controlling the chronic phase disease is tempered somewhat by the persistence of the LSC pool in the majority of the patients. Combined therapies targeting aberrant properties of LSC may obviate therapeutic resistance and relapse in advanced phase and therapeutically recalcitrant CML.
Ultrasmooth, Large‐Area, High‐Uniformity, Conductive Transparent Single‐Walled‐Carbon‐Nanotube Films for Photovoltaics Produced by Ultrasonic Spraying (2009)

Single-walled-carbon-nanotube (SWNT) films are prepared with high transparency, electrical conductivity, and uniformity, with exceptionally low surface roughness, on arbitrarily large substrates by ultrasonic spraying. This scalable process is ideally suited for large-area, solution-based production of SWNT electrodes for photovoltaics and other optoelectronic applications. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Efficient clustering of high-dimensional data sets with application to reference matching (2000)

important problems involve clustering large datasets. Although naive implementations of clustering are computa- tionally expensive, there are established ecient techniques for clustering when the dataset has either (1) a limited num- ber of clusters, (2) a low feature dimensionality, or (3) a small number of data points. However, there has been much less work on methods of eciently clustering datasets that are large in all three ways at once|for example, having millions of data points that exist in many thousands of di- mensions representing many thousands of clusters. We present a new technique for clustering these large, high- dimensional datasets. The key idea involves using a cheap, approximate distance measure to eciently divide the data into overlapping subsets we call canopies .T hen cluster- ing is performed by measuring exact distances only between points that occur in a common canopy. Using canopies, large clustering problems that were formerly impossible become practical. Under reasonable assumptions about the cheap distance metric, this reduction in computational cost comes without any loss in clustering accuracy. Canopies can be applied to many domains and used with a variety of cluster- ing approaches, including Greedy Agglomerative Clustering, K-means and Expectation-Maximization. We present ex- perimental results on grouping bibliographic citations from the reference sections of research papers. Here the canopy approach reduces computation time over a traditional clus- tering approach by more than an order of magnitude and decreases error in comparison to a previously used algorithm by 25%.
In vitro and In vivo Antitumor Effects of the Dual Insulin-Like Growth Factor-I/Insulin Receptor Inhibitor, BMS-554417 (2006)

The insulin-like growth factor receptor (IGF-IR) and insulin receptor are either overactivated and/or overexpressed in a wide range of tumor types and contribute to tumorigenicity, proliferation, metastasis, and drug resistance. Here, we show that BMS-554417, a novel small molecule developed as an inhibitor of IGF-IR, inhibits IGF-IR and insulin receptor kinase activity and proliferation in vitro, and reduces tumor xenograft size in vivo. In a series of carcinoma cell lines, the IC50 for proliferation ranged from 120 nmol/L (Colo205) to >8.5 micromol/L (OV202). The addition of stimulatory ligands was unnecessary for the antiproliferative effect in MCF-7 and OV202 cells. BMS-554417 treatment inhibited IGF-IR and insulin receptor signaling through extracellular signal-related kinase as well as the phosphoinositide 3-kinase/Akt pathway, as evidenced by decreased Akt phosphorylation at Ser473. At doses that inhibited proliferation, the compound also caused a G0-G1 arrest and prevented nuclear accumulation of cyclin D1 in response to LR3 IGF-I. In Jurkat T-cell leukemia cells, this agent triggered apoptotic cell death via the mitochondrial pathway. BMS-554417 was orally bioavailable and significantly inhibited the growth of IGF1R-Sal tumor xenografts in vivo. BMS-554417 is a member of a novel class of IGF-IR/insulin receptor inhibitors that have potential clinical applications because of their antiproliferative and proapoptotic activity in vitro and in vivo.
Electrospun Nanofibrous Membranes for Highly Sensitive Optical Sensors (2002)

The first use of electrospun nanofibrous membranes as highly responsive fluorescence quenching-based optical sensors for metal ions (Fe3+ and Hg2+) and 2,4-dinitrotoluene (DNT) is reported. A fluorescent polymer, poly(acrylic acid)−poly(pyrene methanol) (PAA−PM), was used as a sensing material. Optical chemical sensors were fabricated by electrospinning PAA−PM and thermally cross-linkable polyurethane latex mixture solutions. These sensors showed high sensitivities due to the high surface area-to-volume ratio of the nanofibrous membrane structures.
Health and safety in the office: 3 (1985)

Radiation from VDUs ▪ The problem VDUs emit low levels of radiation, and there was a scare in North America recently that this had been the cause of higher than usual incidences of miscarriages and birth defects among VDU operators. Some organisations took this sufficiently seriously as to make all female operators of child‐bearing age wear protective aprons. But our own Health and Safety Executive has concluded recently that there is no significant risk. A major research project has been launched in the US, however, so obviously the worry still persists. Television sets carry the same risk but only if you sit within four or five feet of them. The radiation is ineffective beyond this distance.
Measurement of propagation constant in waveguides with wideband coherent terahertz spectroscopy (2003)

A quasi-optical technique for characterizing micromachined waveguides is demonstrated with wideband time-resolved terahertz spectroscopy. A transfer-function representation is adopted for the description of the relation between the signals in the input and output port of the waveguides. The time-domain responses were discretized, and the waveguide transfer function was obtained through a parametric approach in the z domain after describing the system with an autoregressive with exogenous input model. The a priori assumption of the number of modes propagating in the structure was inferred from comparisons of the theoretical with the measured characteristic impedance as well as with parsimony arguments. Measurements for a precision WR-8 waveguide-adjustable short as well as for G-band reduced-height micromachined waveguides are presented.
Serum Cytokine Profile in Patients with Chronic Rhinosinusitis with Nasal Polyposis Infected by Aspergillus flavus (2017)

High levels of IL-17 and its regulatory cytokines in patients with chronic rhinosinusitis with nasal polyposis infected by Aspergillus flavus raise a concern about effective disease management and therapeutic recovery. Surgical removal of the nasal polyp being the chief management option, the choice of post-operative drugs may differ in eosinophilic vs. non-eosinophilic nasal polyposis. The prognosis is likely poor, warranting extended care.
Functional Analysis of PA Binding by Influenza A Virus PB1: Effects on Polymerase Activity and Viral Infectivity (2001)

Influenza A virus expresses three viral polymerase (P) subunits-PB1, PB2, and PA-all of which are essential for RNA and viral replication. The functions of P proteins in transcription and replication have been partially elucidated, yet some of these functions seem to be dependent on the formation of a heterotrimer for optimal viral RNA transcription and replication. Although it is conceivable that heterotrimer subunit interactions may allow a more efficient catalysis, direct evidence of their essentiality for viral replication is lacking. Biochemical studies addressing the molecular anatomy of the P complexes have revealed direct interactions between PB1 and PB2 as well as between PB1 and PA. Previous studies have shown that the N-terminal 48 amino acids of PB1, termed domain alpha, contain the residues required for binding PA. We report here the refined mapping of the amino acid sequences within this small region of PB1 that are indispensable for binding PA by deletion mutagenesis of PB1 in a two-hybrid assay. Subsequently, we used site-directed mutagenesis to identify the critical amino acid residues of PB1 for interaction with PA in vivo. The first 12 amino acids of PB1 were found to constitute the core of the interaction interface, thus narrowing the previous boundaries of domain alpha. The role of the minimal PB1 domain alpha in influenza virus gene expression and genome replication was subsequently analyzed by evaluating the activity of a set of PB1 mutants in a model reporter minigenome system. A strong correlation was observed between a functional PA binding site on PB1 and P activity. Influenza viruses bearing mutant PB1 genes were recovered using a plasmid-based influenza virus reverse genetics system. Interestingly, mutations that rendered PB1 unable to bind PA were either nonviable or severely growth impaired. These data are consistent with an essential role for the N terminus of PB1 in binding PA, P activity, and virus growth.
Wavelength-selective Switch for Few-mode Fiber Transmission (2013)

We demonstrate combined WDM/MDM transmission over a novel few-mode fiber based 1 × 9 wavelength-selective switch that supports fibers with 6 spatial and polarization modes. The effect on transmission of cascaded passband filtering and mode-dependent loss build-up is investigated in detail.
Inflammatory mediators promote production of shed LRP1/CD91, which regulates cell signaling and cytokine expression by macrophages (2010)

Abstract The shed form of LRP1/CD91, which is generated at increased levels in inflammation, regulates cell-signaling and cytokine expression by macrophages. LRP1 is a type-1 transmembrane receptor that mediates the endocytosis of diverse ligands. LRP1 β-chain proteolysis results in release of sLRP1 that is present in human plasma. In this study, we show that LPS and IFN-γ induce shedding of LRP1 from RAW 264.7 cells and BMMs in vitro. ADAM17 was principally responsible for the increase in LRP1 shedding. sLRP1 was also increased in vivo in mouse plasma following injection of LPS and in plasma from human patients with RA or SLE. sLRP1, which was purified from human plasma, and full-length LRP1, purified from mouse liver, activated cell signaling when added to cultures of RAW 264.7 cells and BMMs. Robust activation of p38 MAPK and JNK was observed. The IKK-NF-κB pathway was transiently activated. Proteins that bind to the ligand-binding clusters in LRP1 failed to inhibit sLRP1-initiated cell signaling, however an antibody that targets the sLRP1 N terminus was effective. sLRP1 induced expression of regulatory cytokines by RAW 264.7 cells, including TNF-α, MCP-1/CCL2, and IL-10. These results demonstrate that sLRP1 is generated in inflammation and may regulate inflammation by its effects on macrophage physiology.