gebhart/patrepeval huggingface dataset:
Plasma Aldosterone Concentrations Are Not Related to the Degree of Angiotensin-Converting Enzyme Inhibition in Essential Hypertensive Patients. (2000)

There is increasing evidence of important cardiovascular effects of aldosterone via classical mineralocorticoid receptors in the heart. Aldosterone plus excess salt administration has been shown to produce both cardiac hypertrophy and cardiac fibrosis in rats. Various clinical studies have reported that aldosterone plays an important role in cardiac hypertrophy; however, the factors that control plasma aldosterone concentrations during angiotensin-convening enzyme (ACE) inhibitor treatment have still not been established. In the present study, we examined the relationship between plasma aldosterone concentrations and the degree of ACE inhibition in 25 essential hypertensive patients treated with an ACE inhibitor. Blood pressure decreased with treatment and plasma ACE activity, estimated in vitro (by a colorimetric method) and in vivo (by plasma angiotensin II/angiotensin I ratio) assay, was suppressed compared with that of hypertensive patients treated with medication other than ACE inhibitors. No relationship was found between the level of ACE inhibition and plasma aldosterone concentrations, which rose in parallel with the duration of ACE inhibitor treatment. The present study demonstrates that continuous ACE inhibitor therapy produces significant suppression of plasma ACE activity in essential hypertensive patients, but that no relationship exists between plasma aldosterone concentrations and levels of ACE inhibition. Plasma aldosterone concentrations tend to increase with the duration of ACE inhibitor treatment, although this increase did not reflect a reduced inhibition of ACE activity. (Hypertens Res 2000; 23: 25-31)
Identification of a Family of Mastermind-Like Transcriptional Coactivators for Mammalian Notch Receptors (2002)

The molecular mechanisms by which Notch receptors induce diverse biological responses are not fully understood. We recently cloned a mammalian homologue of the Mastermind gene of Drosophila melanogaster, MAML1 (Mastermind-like-1 molecule) and determined that it functions as a transcriptional coactivator for Notch receptors. In this report, we characterize two additional genes in this Mastermind-like gene family: MAML2 and MAML3. The three MAML genes are widely expressed in adult tissues but exhibit distinct expression patterns in mouse early spinal cord development. All MAML proteins localize to nuclear bodies, share a conserved basic domain in their N termini that binds to the ankyrin repeat domain of Notch, and contain a transcriptional activation domain in their C termini. Moreover, as determined by using coimmunoprecipitation assays, each MAML protein was found to be capable of forming a multiprotein complex with the intracellular domain of each Notch receptor (ICN1 to -4) and CSL in vivo. However, MAML3 bound less efficiently to the ankyrin repeat domain of Notch1. Also, in U20S cells, whereas MAML1 and MAML2 functioned efficiently as coactivators with each of the Notch receptors to transactivate a Notch target HES1 promoter construct, MAML3 functioned more efficiently with ICN4 than with other forms of ICN. Similarly, MAML1 and MAML2 amplified Notch ligand (both Jagged2 and Delta1)-induced transcription of the HES-1 gene, whereas MAML3 displayed little effect. Thus, MAML proteins may modify Notch signaling in different cell types based on their own expression levels and differential activities and thereby contribute to the diversity of the biological effects resulting from Notch activation.
Portable device for live tissue imaging (2006)

ADVERTISEMENT RETURN TO ISSUEPREVINSTRUMENTALSNEXTPortable device for live tissue imagingSteve MillerCite this: Anal. Chem. 2006, 78, 1, 17Publication Date (Web):January 1, 2006Publication History Published online1 January 2006Published inissue 1 January 2006https://pubs.acs.org/doi/10.1021/ac069345bhttps://doi.org/10.1021/ac069345bnewsACS Publications. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views230Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (82 KB) Get e-Alertsclose SUBJECTS:Imaging Get e-Alerts
Image-Feature Extraction for Protein Crystallization: Integrating Image Analysis and Case-Based Reasoning (2001)

This paper describes issues related to integrating image anal-ysis techniques into case-based reasoning. Although the ap-proach is generic, a high-throughput protein crystallization problem is used as an example. Our solution to the crystal-lization problem is to store outcomes of experiments as im-ages, extract important image features, and use them to auto-matically recognize different crystallization outcomes. Sub-sequently, we use the outcomes of image classification to per-form case-based planning of crystallization experiments for new proteins. Knowledge-discovery techniques are used to extract general principles for crystallization. Such principles are applicable to the adaptation phase of case-based reason-ing. The motivation for automated image-feature extraction is twofold: (1) the human interpretation/analysis of image content is subjective, and (2) many problem domains require reasoning with large databases of uninterpreted images. In this paper we present the design and implementation of our integrated system, as well as some preliminary experimental results.
A Practical Synthesis of C2-Symmetric Chiral Binaphthyl Ketone Catalyst (2000)

A practical synthesis of 11-membered C 2-symmetric binaphthyl ketone (R)-1, a catalyst for asymmetric epoxidation, was developed. (±)-1,1'-Binaphthyl-2,2'-dicarboxylic acid [(±)-6] was efficiently resolved by (R)-(-)-1-cyclohexylethylamine to give (R)-6 in > 99% ee and in 38% yield. Condensation of the acid chloride derived from (R)-6 with 1,3-dihydroxyacetone dimer at 60-70 °C provided the desired chiral ketone (R)-1 in 61-63% yield without need for high dilution techniques.
A Min-Cut Placement Algorithm for General Cell Assemblies Based on a Graph Representation (1979)

A new placement algorithm for general cell assemblies is presented which combines the ideas of polar graph representation and min-cut placement. First a detailed description of the initial placement procedure is given, then the various methods for placement improvement (rotation, squeezing, reflecting) and global routing are discussed. A sample circuit is used to demonstrate the performance of the algorithms. Results are shown to compare favourably with manually achieved solutions.
Steroids, histamine, and serotonin in the medicinal leech salivary gland secretion (2008)

Lipids represent 20% of the total weight of dried pool of medicinal leech salivary gland secretion (SGS) received from about 50 individual animals. There were detected steroids, but not pospholipids in SGS lipid fraction. Immunochemiluminescent analysis identified free steroid hormones in SGS: cortisol, progesterone, testosterone, estradiol and dehydroepiandrosterone. Microchromatografic-mass-spectrometric analysis of SGS and of low-molecular weight fraction (LMW) (molecular masses ranged from 220 to 850 Da) has shown the multicomponent nature of the LMW fraction. Using standart preparations as the reference steroid hormones (cortisol, dehydroepiandrosterone, androstenedione and testosterone) and histamine and serotonine have been identified in SGS.
Blockchain as a Service: An Autonomous, Privacy Preserving, Decentralized Architecture for Deep Learning. (2018)

Deep learning algorithms have recently gained attention due to their inherent capabilities and the application opportunities that they provide. Two of the main reasons for the success of deep learning methods are the availability of processing power and big data. Both of these two are expensive and rare commodities that present limitations to the usage and implementation of deep learning. Decentralization of the processing and data is one of the most prevalent solutions for these issues. This paper proposes a cooperative decentralized deep learning architecture. The contributors can train deep learning models with private data and share them to the cooperative data-driven applications initiated elsewhere. Shared models are fused together to obtain a better model. In this work, the contributors can both design their own models or train the models provided by the initiator. In order to utilize an efficient decentralized learning algorithm, blockchain technology is incorporated as a method of creating an incentive-compatible market. In the proposed method, Ethereum blockchain's scripting capabilities are employed to devise a decentralized deep learning mechanism, which provides much higher, collective processing power and grants access to large amounts of data, which would be otherwise inaccessible. The technical description of the mechanism is described and the simulation results are presented.
Age-related macular degeneration: a target for nanotechnology derived medicines (2007)

Despite the fact that the retina is a fairly accessible portion of the central nervous system, there are virtually no treatments for early age-related macular degeneration (AMD). AMD is a degenerative retinal disease that causes progressive loss of central vision and is the leading cause of irreversible vision loss and legal blindness in individuals over the age of 50. Both environmental and genetic components play a role in its development. AMD is a multifactorial disease with characteristics that include drusen, hyperpigmentation and/or hypopigmentation of the retinal pigment epithelium (RPE), geographic atrophy and, in a subset of patients, late-stage choroidal neovascularization (CNV). Drugs that inhibit vascular endothelial growth factor (VEGF) have proven effective in treating late-stage CNV, but optimal means of drug delivery remains to be determined. Microscopic particles, whose size is on the nanometer scale, show considerable promise for drug delivery to the retina, for gene therapy, and for powering prosthetic "artificial retinas." This article summarizes the pathophysiology of AMD stressing potential applications from nanotechnology.
Surfactants in Personal Care Products and Decorative Cosmetics (2006)

From anti-aging creams to make-up, surfactants play a key role as delivery systems for skin care and decorative cosmetic products. Surfactants in Personal Care Products and Decorative Cosmetics, Third Edition presents a scientific basis in surfactant science and recent advances in the industry necessary for understanding, formulating, and te
Degradation of N -Nitrosamines by Intestinal Bacteria (1975)

A major proportion of bacterial types, common in the gastrointestinal tract of many animals and man, were active in degrading diphenylnitrosamine and dimethylnitrosamine, the former being degraded more rapidly than the latter. At low nitrosamine concentrations (is less than 0.05 micronmol/ml), approximately 55% of added diphenylnitrosamine, 30% of N-nitrosopyrrolidine, and 4% of dimethylnitrosamine were degraded. The route of nitrosamine metabolism by bacteria appears to be different from that proposed for breakdown by mammalian enzyme systems in that carbon dioxide and formate were not produced. In bacteria, the nitrosamines were converted to the parent amine and nitrite ion and, in addition, certain unidentified volatile metabolites were produced from dimethylnitrosamine by bacteria. The importance of bacteria in reducing the potential hazard to man of nitrosamines is discussed.
Prognostic and Clinicopathologic Associations of Oncogenic BRAF in Metastatic Melanoma (2011)

V600K mutations comprised 20% of BRAF mutations. Characteristics of the antecedent primary melanoma and age at diagnosis differed in BRAF-mutant and BRAF wild-type patients. The presence of mutant BRAF had no impact on the disease-free interval from diagnosis of first-ever melanoma to first distant metastasis; however, it may have impacted survival thereafter.
Flow velocity measurements by frequency domain short coherence interferometry (2002)

A method to measure the longitudinal flow velocity component based on phase resolved frequency domain optical coherence tomography (FDOCT) is introduced. At a center wavelength of 800nm the accessible velocity components ranges from 2 micrometers /s up to 2 mm/s. The upper limit is set by half the maximum frame rate of the CCD detector array. The lower limit is determined by the minimum resolvable phase change in the system, which is set by the system phase noise of 1 deg. First tests of the method include the velocity measurement of a mirror mounted on an oscillating piezo translator, and the flow of 8 micrometers latex spheres dispersed in water through a glass capillary.
The in vitro influences of neurotensin on the motility characteristics of human U373 glioblastoma cells (2006)

Astrocytic tumours are associated with dismal prognoses due to their pronounced ability to diffusely invade the brain parenchyma. Various neuropeptides, including gastrin, are able to modulate tumour astrocyte migration. While neurotensin has been shown to influence the proliferation of glioma cells and the migratory ability of a large set of other cell types, its role in glioma cell migration has never been investigated. Neurotensin-induced modifications to the motility features of human U373 glioblastoma cells therefore constitute the topic of the present study. We evidenced that three subtypes of neurotensin receptors (NTR1, NTR2 and NTR3) are expressed in U373 glioblastoma cells, at least as far as their mRNAs are concerned. Treating U373 tumour cells with 10 nM neurotensin markedly modified the morphological patterns of these cells and also profoundly altered the organization of their actin cytoskeletons. Pull-down assays revealed that neurotensin induced the activation in U373 cells of both Rac1 and Cdc42 but not RhoA. Scratch wound assays evidenced that neurotensin (0.1 and 10 nM) very significantly inhibited wound colonization by U373 cells cultured in the absence of serum. In addition, quantitative phase-contrast videomicroscopy analyses showed that neurotensin decreases the motility levels of U373 glioblastoma cells when these cells are cultured on plastic. In sharp contrast, neurotensin stimulates the motility of U373 cells when they are cultured on laminin, which is a pro-adhesive extracellular matrix component ubiquitously secreted by glioma cells. Our data thus strongly suggest that, in addition to gastrin, neurotensin is a neuropeptide capable of modulating tumour astrocyte migration into the brain parenchyma.
Refractive index and elastic properties of z-cut quartz shocked to 60 kbar (2000)

Shock wave experiments were performed to characterize z-cut, α-quartz as an optical window for velocity interferometry measurements. Refractive index changes and shock velocities were determined to 60 kbar peak stress. Results indicate that the window correction to velocity measurements is a constant fraction of the actual particle velocity, Δu/u=0.081 07. Shock velocity measurements provide the Hugoniot curve to 60 kbar, and yield the fourth-order elastic constant, C3333=174 810 kbar. By combining the velocity correction factor and the shock velocity measurements, the refractive index is determined to be a linear function of density. This finding is used to show that the velocity correction appropriate for a step jump may be applied to monotonically time-dependent wave forms with negligible error. The present work has demonstrated the use of z-cut quartz as an optical window to 60 kbar.
Image quality of scintillator-based x-ray electronic imagers (1998)

A number of new concepts are presently appearing for large size X-ray imaging. The almost perfect MTF obtained with photoconductors read by an a-Si matrix is undoubtedly the best as regards signal, but the associated aliasing effects force the total photon noise power into the useful spatial frequency range, which degrades the signal to noise ratio. At present, an imager consisting of a CsI:Tl scintillator layer coupled to an array of a-Si pixels is the most attractive concept because it combines (1) A strong X-ray absorption together with a good MTF, (2) The best overall X-ray to electrons conversion factor, necessary to overcome electronic noises, (3) A low pass filtering effect which strongly attenuates photon noise beyond the Nyquist limit. The variation of DQE with spatial frequency is the right factor of merit for an X-ray imager. It not only accounts for the spatial resolution, but also contains the effects of all noises in the detection process, including aliasing. Whenever a digital imager is compared with an analogue detector such as the screen/film, the effect of the position of the object with respect to the pixel lattice (or phase difference between the sampling pitch a and the spatial frequency of interest f) must be taken into account. This results in an additional sinc<SUP>2</SUP>(fa) factor applied to the DQE(f).
A Directly Spliced Exon 10–Containing CD44 Variant Promotes the Metastasis and Homotypic Aggregation of Aggressive Non-Hodgkin's Lymphoma (1998)

Variants of the CD44 cell-surface adhesion molecule include additional sequences encoded by combinations of exons from the membrane proximal domain (exons 6-14). Preliminary studies suggest that these additional variable membrane proximal sequences may alter the ligand specificity, glycosylation, and biologic function of CD44. In earlier studies, we found that primary extranodal and widely disseminated aggressive non-Hodgkin's lymphomas (NHLs) and normal activated B cells expressed a directly spliced exon 10-containing variant (CD44ex10), whereas normal resting B cells expressed larger exon 10-containing variants (CD44ex10-14 and CD44ex7-14). To obtain additional information regarding the function of exon 10-containing CD44 variants in aggressive NHL, we generated aggressive NHL transfectants that expressed CD44ex10, CD44ex10-14, CD44ex7-14, the standard CD44 isoform (CD44H), or vector alone, and evaluated the local tumorogenicity, aggregation, and metastatic potential of these transfectants. CD44ex10 aggressive NHL transfectants were more likely to cause local tumor formation in nude mice than transfectants expressing the larger exon 10-containing variants, CD44H, or vector alone. In addition, cell suspensions derived from CD44ex10 local tumors exhibited far greater homotypic aggregation than those obtained from other CD44 or vector-only local tumors. In nude mice that received CD44ex10 transfectants, distant metastases were also significantly more likely to develop than in animals that were given either the CD44ex10-14, CD44ex7-14, CD44H, or vector-only transfectants. These data provide the first evidence that the directly spliced exon 10-containing CD44 variant (CD44ex10) has a unique biologic function in aggressive NHL.
Regulation of HILDA/LIF gene expression in activated human monocytic cells (1991)

We have previously shown that human monocytes express HILDA/LIF gene and its protein product upon stimulation. In the present study we have investigated some of the possible mechanisms involved in the regulation of HILDA/LIF gene expression in activated human monocytes and compared them to those of granulocyte colony-stimulating factor (G-CSF) and IL-1 beta gene expression. In the absence of added stimuli HILDA/LIF mRNA was barely detectable in isolated monocytes. HILDA/LIF mRNA accumulation was weakly induced by stimuli such as LPS or phorbol ester. However, a synergy was observed when each stimulus was used in combination with 1,25-dihydroxyvitamin D3. Nuclear run-on analysis did not detect an increase in HILDA/LIF gene transcription upon stimulation with LPS or phorbol ester in combination with 1,25-dihydroxyvitamin D3. HILDA/LIF mRNA half-life showed an increase when phorbol ester and 1,25-dihydroxyvitamin D3 were used in combination over that obtained for each stimuli alone. HILDA/LIF mRNA expression was largely inhibited when monocytes were stimulated in the presence of cycloheximide (CHX) added either at the onset or 4 h after the beginning of the stimulation period. When CHX was added at later time points, 2 h before cell harvesting, HILDA/LIF mRNA levels were superinduced when compared with those in cells stimulated in the absence of CHX. This superinduction can be at least partially explained by post-transcriptional mechanisms, since HILDA/LIF mRNA half-life in CHX-superinduced cells was significantly increased when compared with values on stimulated CHX-untreated cells. In contrast to HILDA/LIF, G-CSF and IL-1 beta mRNA accumulation showed a different response pattern to the same stimuli, and a augmentation of their gene transcription was detected by the run-on technique in activated monocytic cells when compared with controls. Furthermore, G-CSF and Il-1 beta mRNA levels were superinduced in monocytes stimulated in the presence of CHX from the onset of the stimulation period. These studies indicate that, when compared to G-CSF and IL-1 beta, the expression of HILDA/LIF gene is under the control of relatively specific regulatory mechanisms. HILDA/LIF gene expression is controlled by newly synthesized proteins acting at early and late time points of the stimulation period and with opposite effects on HILDA/LIF mRNA levels. Finally, HILDA/LIF mRNA levels are regulated by post-transcriptional mechanisms of mRNA stabilization probably controlled through labile newly synthesized proteins.
Aberrant methylation of suppressor of cytokine signalling-1 (SOCS-1) gene in pancreatic ductal neoplasms (2003)

The suppressor of cytokine signalling-1 (SOCS-1) gene is frequently silenced in human hepatocellular carcinoma by aberrant methylation. The aim of this study was to determine if SOCS-1 is inactivated in pancreatic ductal neoplasms, and to investigate if aberrant methylation of this gene affected the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. Aberrant methylation in the CpG island of the SOCS-1 gene was detected in six of 19 (31.6%) human pancreatic cancer cell lines using methylation-specific PCR, and was associated with a loss or reduction of gene expression in five of the six methylated cell lines. Thirteen of 60 pancreatic ductal adenocarcinomas (21.7%) and two of 34 intraductal papillary mucinous neoplasms (IPMNs) (5.9%) had methylated SOCS-1. In contrast, SOCS-1 methylation was not seen in pancreatic normal ductal epithelia (zero out of 15), in pancreatic intraepithelial neoplasia (PanINs) (zero out of 49) or in the IPMNs without infiltrating cancer (zero out of 20). 5-Aza-2'-deoxycytidine treatment of the SOCS-1-methylated pancreatic cancer cell lines led to restoration of SOCS-1 gene expression. Interleukin-6, which has been shown to act through the JAK/STAT pathway to increase cell growth, induced modest time and dose-dependent cell proliferation in a SOCS-1-methylated cell line (PL10, P=0.015) but not in two unmethylated cell lines. These results indicate that loss of SOCS-1 gene is associated with transcriptional silencing and may have growth-promoting effects, and that its methylation is a useful marker of pancreatic cancer.