gebhart/patrepeval huggingface dataset:
DC to AC inverter with improved one cycle control (2004)

Switch mode DC to AC inverters using DC-to-DC converter topology has many advantages over the traditional bridge-type inverter. One cycle control is proposed to form a high performance inverter, but high performance ability was need for the magnify integrated chip. In this paper the control circuit error near the zero crossing point was magnified and steady state point of the magnifier was changed from zero to a positive number to minimized the distortion near the zero crossing point. Because one cycle control method was not acute to the load changed, a proportional loop was used and voltage feedback archived from the filter end. And the experimental results are given out.
Astrocytic necrosis is induced by anti-aquaporin-4 antibody-positive serum (2009)

Neuromyelitis optica (NMO) is an inflammatory demyelinating disease of the central nervous system, and humoral immunity is suggested to play an important role in the pathogenesis. The identification of an anti-aquaporin-4 antibody (AQP4-Ab, neuromyelitis optica immunoglobulin G) in the sera of patients with NMO has led to the investigation on the pathogenicity of the autoantibody. Recent immunohistological analyses revealed the primary loss of AQP4 on astrocytes and complement deposition in active lesions of NMO. In this report, we show that astrocytes are susceptible to sera from AQP4-Ab-positive patients and undergo necrosis in a complement-dependent manner. Our results suggest the primary pathogenic role of AQP4-Ab in NMO.
Leukocyte-derived matrix metalloproteinase-9 mediates blood-brain barrier breakdown and is proinflammatory after transient focal cerebral ischemia (2005)

Results of recent studies reveal vascular and neuroprotective effects of matrix metalloproteinase-9 (MMP-9) inhibition and MMP-9 gene deletion in experimental stroke. However, the cellular source of MMP-9 produced in the ischemic brain and the mechanistic basis of MMP-9-mediated brain injury require elucidation. In the present study, we used MMP-9-/- mice and chimeric knockouts lacking either MMP-9 in leukocytes or in resident brain cells to test the hypothesis that MMP-9 released from leukocytes recruited to the brain during postischemic reperfusion contributes to this injury phenotype. We also tested the hypothesis that MMP-9 promotes leukocyte recruitment to the ischemic brain and thus is proinflammatory. The extent of blood-brain barrier (BBB) breakdown, the neurological deficit, and the volume of infarction resulting from transient focal stroke were abrogated to a similar extent in MMP-9-/- mice and in chimeras lacking leukocytic MMP-9 but not in chimeras with MMP-9-containing leukocytes. Zymography and Western blot analysis from these chimeras confirmed that the elevated MMP-9 expression in the brain at 24 h of reperfusion is derived largely from leukocytes. MMP-9-/- mice exhibited a reduction in leukocyte-endothelial adherence and a reduction in the number of neutrophils plugging capillaries and infiltrating the ischemic brain during reperfusion; microvessel immunopositivity for collagen IV was also preserved in these animals. These latter results document proinflammatory actions of MMP-9 in the ischemic brain. Overall, our findings implicate leukocytes, most likely neutrophils, as a key cellular source of MMP-9, which, in turn, promotes leukocyte recruitment, causes BBB breakdown secondary to microvascular basal lamina proteolysis, and ultimately contributes to neuronal injury after transient focal stroke.
One-Step Optimization Using Touchdown and Stepdown PCR (2003)

Polymerase chain reaction (PCR) optimization and troubleshooting can consume considerable energy and resources because of the finicky and often unpredictable nature of the reactions. Small variations in any of the many variables in a given reaction can have a pronounced effect on the resultant amplicon profile. Reactions that are too stringent yield negligible product and reactions that are not stringent enough yield artifactual amplicons. Variables include concentrations of Mg2+, H+, dNTPs, primers, and template, as well as cycling parameters. With regard to the latter, the value selected for the annealing temperatures is most critical. Unfortunately, even with the most sophisticated algorithms (i.e., OLIGO) it is often difficult to predict the amplification optima a priori leaving no other choice but to employ empirical determination.
Matrix Rigidity Regulates Cancer Cell Growth and Cellular Phenotype (2010)

These observations suggest that the mechanical properties of the matrix environment play a significant role in regulating the proliferation and the morphological properties of cancer cells. Further, the multiwell format of the soft-plate assay is a useful and effective adjunct to established 3-dimensional cell culture models.
[A study of transgenic IFV cattle integrated with human serum albumin gene]. (2000)

The mammary gland expression vector (pcDNA 3.1-GCALBm) containing the full-length sequence of human serum albumin (hALB) cDNA and intron 1 as well as the goat beta-casien gene promoter and 5' up-stream regulatory sequence was constructed. The vector was micro-injected into bovine IVF eggs. The embryos were in vitro cultured to the late stage of morulae, and then few embryo cells were aspirated for the implantation detection of target gene integration and SRY DNA sequence using nested-PCR. Afterwards, ten integrated embryos were selected to transfer into eight recipients and three were pregnant. The pregnant rate was 37.5%(3/8). However, two were miscarried in mid-trimester but one was pregnant at term to deliver a male transgenic cattle integrated with hALB mini-gene. The transgenic efficiency was 12.5% (1/8).
Photoelectrochemical Properties of InGaN for H2 Generation from Aqueous Water (2005)

The photoelectrochemical properties of In x Ga 1- x N ( x =0.02 and 0.09) were compared with those of GaN. The band-edge potentials of In x Ga 1- x N were determined by the Mott–Schottky plot for the first time. The gas generation from a counterelectrode using the In 0.02 Ga 0.91 N working electrode was the highest of the three samples. Band-edge potentials and the light absorption of a working photoelectrode presumably affect the gas generation efficiency.
Broadband excitation for short-time impedance spectroscopy (2008)

Frequency domain impedance measurements are still the common approach in assessing passive electrical properties of cells and tissues. However, due to the time requirements for sweeping over a frequency range for performing spectroscopy, they are not suited for recovering fast impedance changes of biological objects. The use of broad bandwidth excitation and monitoring the response as a function of time will greatly reduce the measurement time. The widespread usage of a square wave excitation is simple but not always the best choice. Here we consider different waveforms for excitation and discuss not only the advantages but also their limitations. Measurements in a miniaturized chamber where frequency and time domain measurements are compared show the suitability of different waveforms as excitation signals for the measurements of bio-impedance. The chirp excitation has been found to be most promising in terms of frequency range, signal-to-noise ratio and crest factor.
Surface Plasmon Resonance Based Highly Selective Fiber Optic Dopamine Sensor Fabricated Using Molecular Imprinted GNP/SnO2 Nanocomposite (2018)

Fabrication and characterization of a highly selective fiber optic surface plasmon resonance-based dopamine sensor using molecular imprinted graphene nanoaplatelets/tin oxide (SnO2) nanocomposite have been reported. The synthesis of sensing layer has been carried out using a series of optimizations and morphological studies. The effectiveness of the sensing layer over other possible probe designs has been proved by performing control experiments for a dopamine concentration range covering the suggested level of dopamine for human being. The limit of detection (LOD) of the sensor evaluated is 0.031 μM, which is lower than the LOD values of various dopamine sensors fabricated using different methods. The specificity of the sensor for dopamine has been confirmed by performing experiments using various interferands while the stability and reusability of the sensor probe have been checked by performing experiments repeatedly for a long period of time. Apart from high sensitivity, low LOD, and fast response, the sensor can be used for remote sensing and online monitoring of dopamine.
Cloning and characterization of a gene cluster for geldanamycin production inStreptomyces hygroscopicusNRRL 3602 (2003)

We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.
Optimization of an siRNA‐expression system with an improved hairpin and its significant suppressive effects in mammalian cells (2004)

The mutated hairpin siRNAs and their genetically stable coding vectors could be very useful for gene knockdown experiments, and could further benefit gene therapy using RNAi.
An ASIC Control Circuit for Thermal Actuated Large Optical Packet Switch Array (2008)

Abstract—In this article we propose the employment of an ASIC integrated control circuit to compensate the fabrication error and tuning the wavelength response in a thermally actuated ring type optical switch. Additional functionalities can also be added in this circuit by tailoring externally the roundtrip loss or coupling constants of the ring. The design concept can be easily scaled up for large array format optical switch system without much change in the terminal numbers thanks to the three dimensional hierarchy of control circuit design, which effectively reduces the terminal numbers into the cubic root of the total control unit numbers. The integrated circuit has been designed, simulated, as well as fabricated, and demonstrated a decent performance with Free Spectral Range (FSR) equal to 1.5nm at a wavelength of 1534nm and center wavelength shift of the ring up to 0.3nm for thermal actuated ring type optical switch. Index Terms—Optical switch, tuning wavelength, electrical control, actuated, modulation frequency.
Comparative Physiology of Mice and Rats: Radiometric Measurement of Vascular Parameters in Rodent Tissues (2014)

A solid understanding of physiology is beneficial in optimizing drug delivery and in the development of clinically predictive models of drug disposition kinetics. Although an abundance of data exists in the literature, it is often confounded by the use of various experimental methods and a lack of consensus in values from different sources. To help address this deficiency, we sought to directly compare three important vascular parameters at the tissue level using the same experimental approach in both mice and rats. Interstitial volume, vascular volume, and blood flow were radiometrically measured in selected harvested tissues of both species by extracellular marker infusion, red blood cell labeling, and rubidium chloride bolus distribution, respectively. The latter two parameters were further compared by whole-body autoradiographic imaging. An overall good interspecies agreement was observed for interstitial volume and blood flow on a weight-normalized basis in most tissues. In contrast, the measured vascular volumes of most rat tissues were higher than for mouse. Mice and rats, the two most commonly utilized rodent species in translational drug development, should not be considered as interchangeable in terms of vascular volume per gram of tissue. This will be particularly critical in biodistribution studies of drugs, as the amount of drug in the residual blood of tissues is often not negligible, especially for biologic drugs (e.g., antibodies) having long circulation half-lives. Physiologically based models of drug pharmacokinetics and/or pharmacodynamics also rely on accurate knowledge of biological parameters in tissues. For tissue parameters with poor interspecies agreement, the significance and possible drivers are discussed.
Endogenous Testosterone and Mortality Due to All Causes, Cardiovascular Disease, and Cancer in Men (2007)

In men, endogenous testosterone concentrations are inversely related to mortality due to cardiovascular disease and all causes. Low testosterone may be a predictive marker for those at high risk of cardiovascular disease.
Subsarcolemmal and intermyofibrillar mitochondria proteome differences disclose functional specializations in skeletal muscle (2010)

Skeletal muscle is a highly specialized tissue that contains two distinct mitochondria subpopulations, the subsarcolemmal (SS) and the intermyofibrillar (IMF) mitochondria. Although it is established that these mitochondrial subpopulations differ functionally in several ways, limited information exists about the proteomic differences underlying these functional differences. Therefore, the objective of this study was to biochemically characterize the SS and IMF mitochondria isolated from rat red gastrocnemius skeletal muscle. We separated the two mitochondrial subpopulations from skeletal muscle using a refined method that provides an excellent division of these unique mitochondrial subpopulations. Using proteomics of mitochondria and its subfractions (intermembrane space, matrix and inner membrane), a total of 325 distinct proteins were identified, most of which belong to the functional clusters of oxidative phosphorylation, metabolism and signal transduction. Although more gel spots were observed in SS mitochondria, 38 of the identified proteins were differentially expressed between the SS and IMF subpopulations. Compared to the SS mitochondrial, IMF mitochondria expressed a higher level of proteins associated with oxidative phosphorylation. This observation, coupled with the finding of a higher respiratory chain complex activity in IMF mitochondria, suggests a specialization of IMF mitochondria toward energy production for contractile activity.
A Phase-Opposition System of Amplitude Modulation (1938)

The increasing economic importance of power efficiency for high-power broadcast stations is illustrated in Fig. 1 showing that some 50 per cent saving is possible. Different known modulation schemes are compared as regards necessary input power, including the recent Chireix and Doherty systems. The influence of rates for electric power is stressed. A so-called "phase-opposition" system is discussed starting with simple circuit theory, first for seriesand then for parallel-tuned circuits. Principally the system is based on the co-operation of two generators; i.e., one "carrier" generator constantly excited, and one "side-band" generator whose excitation is changed 180 degrees in phase according to the phase of modulation. Mean input power is calculated for this system, giving about the same input as good systems for about 30 per cent modulation, but 10 to 25 per cent more power at 100 per cent modulation. The paper is concluded with a consideration of tube characteristics and possible means for obtaining linearity are shown.
Transplantation of Purified Autologous Fat (1991)

The idea of autologous fat microtransplants has recently resurfaced because of interest by the press. Past experiments have shown that small amounts of fat may be transplanted with an expected survival rate between 10 and 50 percent without ectodermis. A great fund of knowledge exists showing that skin grafts will survive quite dependably if their thickness is up to about 0.0020 inch. For the first week or so, they live by diffusion and inosculation, and then neovascularization enables them to continue viability. I have developed a technique whereby fat is harvested through a blunt cannula with minimal vacuum to prevent explosion of the cellular fat globules. Fibrin and cellular debris are removed, and the fat globules are separated from the free fat, blood, and other constituents of the aspirate. Individual, free-floating 1- to 3-mm adipose fragments are then suspended in nutrient solution and injected through an 18-gauge needle. Multiple radial pathways, with each fat segment being separated from the other by host tissue, maximizes the host-prosthesis interface and the possibility for exchange of nutrients. I have attempted this procedure in more than 100 patients with widely varying results. In acne pits of the face, no significant improvement could be noted 6 weeks after injection. For the first few weeks, a near-perfect result was obtained, but none of these transplants in scarred areas of this kind have been of benefit. Injection of 10 to 50 cc in other areas has resulted in some cells (perhaps 10 percent) surviving over 2 years.(ABSTRACT TRUNCATED AT 250 WORDS)
Microchip-Based Purification of DNA from Biological Samples (2003)

A microchip solid-phase extraction method for purification of DNA from biological samples, such as blood, is demonstrated. Silica beads were packed into glass microchips and the beads immobilized with sol-gel to provide a stable and reproducible solid phase onto which DNA could be adsorbed. Optimization of the DNA loading conditions established a higher DNA recovery at pH 6.1 than 7.6. This lower pH also allowed for the flow rate to be increased, resulting in a decrease in extraction time from 25 min to less than 15 min. Using this procedure, template genomic DNA from human whole blood was purified on the microchip platform with the only sample preparation being mixing of the blood with load buffer prior to loading on the microchip device. Comparison between the microchip SPE (microchipSPE) procedure and a commercial microcentrifuge method showed comparable amounts of PCR-amplifiable DNA could be isolated from cultures of Salmonella typhimurium. The greatest potential of the microchipSPE device was illustrated by purifying DNA from spores from the vaccine strain of Bacillus anthracis, where eventual integration of SPE, PCR, and separation on a single microdevice could potentially enable complete detection of the infectious agent in less than 30 min.
Susceptibility of Campylobacter jejuni to strain-specific bactericidal activity in sera of infected patients (1986)

Campylobacter jejuni is a common cause of inflammatory enteritis, which in normal hosts is usually self-limited and resolves without antibiotic therapy. C. jejuni bacteremia is very rare. We examined sera for bactericidal activity that might be important in limiting the extent of C. jejuni infection in man. We studied the ability of nonimmune sera and homologous and heterologous immune sera from infected patients to kill different fresh case isolates of C. jejuni in vitro. The reduction of the log10 concentration of viable C. jejuni (log10 killing) by fresh sera from nonimmune donors was only 0.2. Log10 killing by homologous acute sera varied from 0 to 3.8 (mean, 1.8). Convalescent sera showed remarkable log10 killing of only homologous C. jejuni, with values of 2.7 to 4.4 (mean, 3.7). The bactericidal effects of acute and convalescent sera were abrogated by heat and EDTA chelation, indicating mediation by complement. The role of classical complement pathway activation was supported by chelating sera with magnesium EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N, N, N', N'-tetraacetic acid] and by reconstitution experiments with heat-inactivated sera, C2-deficient serum, and purified C2. The requirement of specific antibody for the serum bactericidal effect was indicated by the loss of bactericidal activity when immune sera were absorbed with homologous but not heterologous whole C. jejuni isolates. The presence of specific antibodies was further documented by agglutination of only homologous C. jejuni suspensions by heat-inactivated immune sera. Studies with polymorphonuclear leukocytes suggested that ingestion and killing of two C. jejuni strains were modest and variable in the presence of heat-inactivated homologous serum. In summary, the data document a potent serum bactericidal effect that develops rapidly and specifically during C. jejuni enteritis and may be an important factor in host defense against C. jejuni.
Molecular analysis of the von Hippel-Lindau disease tumor suppressor gene in human lung cancer cell lines. (1994)

The deletion of the short arm of chromosome 3 is frequently observed in lung cancer. To determine whether the von Hippel-Lindau (VHL) disease tumor suppressor gene located at 3p25 is responsible for oncogenesis in lung cancer, we searched the known open reading frame using the single-strand conformation polymorphism (SSCP) technique for mutations in the VHL gene in 72 cancer cell lines including small cell (SCLC) and non-small cell (NSCLC) lung cancers, carcinoids, and mesotheliomas. SSCP analysis showed that four cell lines have altered SSCP patterns within the coding region and one in an intron of the VHL gene. SCLC line NCI-H1672 had a somatic mutation, G to A at nucleotide (nt) 530, leading to amino acid substitution (glycine to aspartic acid) compared to normal DNA from the same patient. Mesothelioma line NCI-H28 had T to A mutation at nt 479 leading to leucine to histidine amino acid change. We found one frequent polymorphism A (0.72) or G (0.28) at nt 19 resulting in either serine or glycine at this position, changes also found in normal peripheral blood cell DNA, often in a heterozygous state. In addition, we found single rare polymorphisms which did not alter the coding region including: C to G at nt 396, G to T at nt 843, and C to T change in an intron. These results suggest that the VHL gene is only rarely mutated in thoracic malignancies.
Main-Duct Intraductal Papillary Mucinous Neoplasms of the Pancreas (2004)

Cancer is found in 60% of patients with main-duct IPMNs. Patients with malignant tumors are 6 years older than their benign counterparts and have a higher likelihood of presenting with jaundice or new onset diabetes. No patients with benign tumors or carcinoma in situ died of their disease following resection, and those with invasive cancer had a markedly better survival (60% at 5 years) than pancreatic ductal adenocarcinoma. These findings support both the concept of progression of benign IPMNs to invasive cancer and an aggressive policy of resection at diagnosis.
Supporting personal mobility for nomadic computing over the internet (1997)

This paper presents a new paradigm for nomadic computing over the Internet called universal personal computing (UPC), where mobile users can access computing resources, network services, and personalized computing environments anywhere using any available terminals. The concept of UPC and system design issues are discussed, and the required system architecture capable of managing different mobile objects, i.e., users and terminals, in the UPC environment is presented. Modifications of connection setup procedures between user application programs to enable addressing based on a global user identity are considered.