gebhart/patrepeval huggingface dataset:
Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization (2000)

The surface-induced mineralization (SIM) technique was used to produce hydroxyapatite (HAP) coatings on external fixation pins with the antimicrobial agent, chlorhexidine, incorporated within the coating. The SIM process involved surface modification of the substrate with organic functional groups followed by immersion in aqueous supersaturated calcium phosphate solutions. X-ray diffraction spectra confirmed that hydroxyapatite coatings were formed. Chlorhexidine was incorporated into the coating by placing the substrate into various chlorhexidine solutions in between mineralization cycles. Total uptake was measured by dissolution of the coating into a 0.1 M nitric acid solution and measuring the chlorhexidine concentration using UV spectroscopy at 251 nm. Release rates were measured by submersion of coated substrates into saline solutions and measuring chlorhexidine UV absorbency at 231 nm as a function of time. Results show an initial rapid release followed by a period of slower sustained release. The anti-microbial efficacy of the HAP-chlorhexidine coatings was evaluated in vitro using a Staphylococcus aureus cell culture. Initial results show a large "inhibition zone" formed around the chlorhexidine/HAP coating vs. coatings with HAP only. This preliminary work clearly demonstrates that SIM HAP coatings have great potential to locally deliver antimicrobial agents such as chlorhexidine at implantation sites, which may greatly reduce the incidence of pin tract infection that occurs in external fixation.
Functional variability of sacral roots in bladder control (2014)

This is the first study showing a significant horizontal and vertical variability in the functional distribution of sacral roots in bladder innervation. These results show the variability of cauda equina syndromes and their forensic implications. These data should help with the monitoring of sacral roots and the performance of several tasks during surgery, including neurostimulation and neuromodulation.
Inhibitory Efficacy of Intravitreal Dexamethasone Acetate-Loaded PLGA Nanoparticles on Choroidal Neovascularization in a Laser-Induced Rat Model (2007)

Purpose: The aims of this study were to investigate the inhibitory efficacy of intravitreal dexamethasone acetate (DA)-loaded by (D, L-lactide-co-glycolide) (PLGA) nanoparticles on choroidal neovascularization (CNV) in a laser-induced rat model and to evaluate its mode of drug release. Methods: DA loaded with PLGA nanoparticles containing 50% DA was prepared using an emulsification/solvent evaporation method. CNV was unilaterally induced by laser photocoagulation in rats. Different dosages of sterilized DA-loaded PLGA nanoparticles suspensions by intravitreal injection were evaluated (i.e., 50, 100, 200, and 400 μg) and the blank PLGA vehicle was the control. The concentration of DA in vitreous was measured by reverse-phase high-performance liquid chromatography (RP-HPLC). Flash electroretinography and transmission electron microscopy were performed to assess retinal toxicity. Fluorescein fundus angiography was performed to evaluate the incidence of CNV on days 14 and 56. The animals were sacrificed on day 56, then eyecups were processed for histologic analysis. Results: The in vitro release of DA from nanoparticles showed that 50% of the drug was released over 2 weeks and controlled release in a linear pattern for 40 days. The pharmacokinetics of DA-loaded PLGA nanoparticles in the eyes with CNV demonstrated a triphasic pattern. The DA concentrations in vitreous remained within the effective range, which is capable of inhibiting inflammatory responses for more than 56 days. On day 14 after photocoagulation, the incidence of CNV was 47.4% as for 50 μg, 28.2% for 100 μg, 15.8% for 200 μg and 7.9% for the 400 μg DA-loaded PLGA nanoparticles group, respectively, and 65.8% for the control. On day 56, The inhibition of DA-loaded PLGA nanoparticles on CNV showed a dose-dependent effect. No sign of retinal toxicity was detected. Conclusions: These results suggest that DA-loaded PLGA nanoparticles can dose-dependently inhibit the development of experimental CNV. The controlled intraocular delivery system of DA-loaded PLGA nanoparticles may be a potentiality for CNV treatment.
Organization of Polymers onto Carbon Nanotubes:  A Route to Nanoscale Assembly (2001)

Atomic scale imaging using scanning tunneling microscopy has been used to investigate the morphology and structure of high molecular weight polymers attached to carbon nanotubes. A surprising degree of regularity is observed in the attached polymers, suggesting a “nanotube-driven” crystallization process is present. The symmetries of this crystalline state of the polymer are directly determined by the underlying nanotube's chirality. Tunneling spectroscopy further demonstrates that the crystallization of the polymer onto the nanotube results in modifications to the nanotube's electronic structure, as would be expected from a strong polymer−tube interaction. The assembly of nanoscale organics into objects with such perfect order clearly foreshadows the construction of large-scale nanoarchitectures with order over many lengths scales.
Anti-CD38 autoantibodies in type? diabetes (2006)

Human diabetes mellitus comprises two main clinical entities: type 1 and type 2 diabetes. While type 1 diabetes is autoimmune in origin, type 2 diabetes is due to a decreased sensitivity to insulin action (so-called insulin resistance) associated with impaired beta cell function. However, it is becoming increasingly clear that there is a certain overlap between these two diseases. While some degree of insulin resistance is present in type 1 diabetic patients, markers of beta cell autoimmunity (either primary or secondary) can frequently be detected in type 2 diabetic subjects. In this scenario, anti-CD38 autoantibodies (aAbs) have been described in both type 1 and type 2 diabetic patients. Contrary to the other known islet aAbs, anti-CD38 autoantibodies are more prevalent in long-standing than in new-onset type 1 diabetes, and more prevalent in type 2 than in type 1 diabetes. Moreover, anti-CD38 aAbs are endowed with unique stimulatory properties on Ca(2+) mobilization and insulin secretion. These observations suggest that autoimmunity may be both the cause and consequence of beta cell dysfunction, in either case imposing a further toll for the control of glucose homeostasis.
PUB-NChIP—“in vivo biotinylation” approach to study chromatin in proximity to a protein of interest (2012)

We have developed an approach termed PUB-NChIP (proximity utilizing biotinylation with native ChIP) to purify and study the protein composition of chromatin in proximity to a nuclear protein of interest. It is based on coexpression of (1) a protein of interest, fused with the bacterial biotin ligase BirA, together with (2) a histone fused to a biotin acceptor peptide (BAP), which is specifically biotinylated by BirA-fusion in the proximity of the protein of interest. Using the RAD18 protein as a model, we demonstrate that the RAD18-proximal chromatin is enriched in some H4 acetylated species. Moreover, the RAD18-proximal chromatin containing a replacement histone H2AZ has a different pattern of H4 acetylation. Finally, biotin pulse-chase experiments show that the H4 acetylation pattern starts to resemble the acetylation pattern of total H4 after the proximity of chromatin to RAD18 has been lost.
Apolipoprotein-E-deficient mice exhibit an increased susceptibility to disseminated candidiasis (2004)

The effect of hyperlipoproteinemia on systemic candidiasis was investigated by assessing the susceptibility of hyperlipoproteinemic, apolipoprotein E (ApoE)-deficient (ApoE -/-) mice to a systemic Candida albicans infection. The absence of ApoE in these mice resulted in an eightfold increase in plasma lipoprotein concentrations in the very low-density lipoprotein (VLDL) fraction, as compared with levels seen in ApoE +/+ mice. Mortality due to candidemia was significantly higher (86%) in ApoE -/- mice than in ApoE+/+ mice (52%), and in platings of homogenized kidney material on fungal culture medium, ApoE -/- mice yielded significantly higher levels of C. albicans outgrowth than did ApoE+/+ mice. C albicans grew twofold better in ApoE -/- plasma in 4 h than in ApoE+/+ plasma, and depletion of lipoproteins from plasma resulted in a significant seven- to tenfold increase in C. albicans growth. Recombinant ApoE did not directly inhibit C. albicans growth. Our data indicate that the increased susceptibility of ApoE -/- mice to C albicans is due both to increased growth of blastoconidia in ApoE -/- mice in response to the availability of lipids as nutrients, and to the neutralization of candidacidal factors by lipoproteins. This study suggests that lipoproteins play a significant role in host defense against candidiasis.
In Situ Copolyesters Containing Poly(l-lactide) and Poly(hydroxyalkanoate) Units (2007)

In situ copolyesters containing polylactide (PLA) and polyhydroxyalkanoate (PHA) segments were obtained via ring-opening polymerization of L-lactide using PHA as a macroinitiator with stannous octoate as catalyst. Incorporation of PHA (20 wt %) into PLA affords a novel copolymer with Mn values ranging from 25 to 50 KDa and low polydispersities of 1.8-2.3. DSC analysis of the copolymer indicates well-defined crystallization and melting transitions different from the homopolymers and corresponding blend. The polymers were characterized by FT-IR, GPC, DSC, optical microscopy, NMR, and TGA. The results show successful reactivity of PHA as a macroinitiator for the ring-opening polymerization of lactide.
Effect of Sample Heterogeneity on the Interpretation of QCM(-D) Data: Comparison of Combined Quartz Crystal Microbalance/Atomic Force Microscopy Measurements with Finite Element Method Modeling (2008)

A quartz crystal microbalance was integrated into an AFM in order to monitor the adsorption of biomolecules to the resonator surface with both atomic force microscopy and microgravimetry. The comparison between the two techniques allows the fractional coverage of the surface, theta, to be correlated with the frequency shift of the resonator, deltaf. The adsorbed material was ferritin, which is a spherical protein with a diameter of approximately 12 nm. Even ata coverage below 50%, the protein layer exhibits Sauerbrey-like behavior, meaning that the magnitude in the frequency shift [deltaf] much exceeds the shift in bandwidth and that the normalized frequency shift, deltaf/n (n the overtone order), is similar on the different overtones. The relation between coverage and frequency shift was found to be nonlinear. In order to model this situation, we performed finite element method calculations based on the incompressible Navier-Stokes equation. The comparison between the model and the experiment suggests that the deformation of the protein upon adsorption is small. For low coverage, the volume of the trapped solvent exceeds the volume of the adsorbate itself. The ratio of the two decreases with increasing coverage. This is the cause of the experimentally observed nonlinear relationship between the surface coverage and frequency shift. Comparing frequency shifts at different overtones, one finds that deltaf/n slightly decreases with overtone order. Such a behavior is typically attributed to softness. The model shows that, for the adsorbed spheres, this apparent softness arises through a rocking motion of the spheres at the surface instead of the shear deformation. Also, there is a hydrodynamic contribution (related to roughness) to the overtone dependence of deltaf/n.
Analysis of multithreaded architectures for parallel computing (1990)

Multithreading has been proposed as an architectural strategy for tolerating latency in multiprocessors and, through limited empirical studies, shown to offer promise. This paper develops an analytical model of multithreaded processor behavior based on a small set of architectural and program parameters. The model gives rise to a large Markov chain, which is solved to obtain a formula for processor efficiency in terms of the number of threads per processor, the remote reference rate, the latency, and the cost of switching between threads. It is shown that a multithreaded processor exhibits three operating regimes: linear (efficiency is proportional to the number of threads), transition, and saturation (efficiency depends only on the remote reference rate and switch cost). Formulae for regime boundaries are derived. The model is embellished to reflect cache degradation due to multithreading, using an analytical model of cache behavior, demonstrating that retums diminish as the number threads becomes large. predictions from the embellished model correlate well with published empirical measurements. prescriptive use of the model under various scenarios indicates that multithreading is effective, but the Ilumber of useful threads per processor is fairly small.
The neuropathic pain: An overview of the current treatment and future therapeutic approaches (2019)

Neuropathic pain is characterized by abnormal hypersensitivity to stimuli (hyperalgesia) and nociceptive responses to non-noxious stimuli (allodynia). The conditions and the pathophysiological states that determine the onset of neuropathic pain are heterogeneous, such as metabolic disorders, neuropathy caused by viral infections, and autoimmune diseases affecting the central nervous system (CNS). Neuropathic pain in the general population is estimated to have a prevalence ranging between 3% and 17%. Most of the available treatments for neuropathic pain have moderate efficacy and present side effects that limit their use; therefore, other therapeutic approaches are needed for patients. In this article, the current standard of care treatment, the emerging pharmacological approaches from the completed phase III clinical trials, and the preclinical studies on novel promising therapeutic options will be reviewed.
Production ofl-Threonine by Analog-resistant Mutants (1972)

Mutants resistant to α-amino-β-hydroxyvaleri0c acid (AHV) were derived from various bacteria which belong to Corynebacterium, Brevibacterium, Arthrobacter, Microbacterium, or Bacillus by mutational treatment with N-methyl-N′-nitro-N-nitrosoguanidine(NTG), and screened for their ability to produce l-threonine. A number of l-threonine producers were obtained from each group of bacteria. Among them, the mutants derived from C. glutamicum KY9159(Met−) were further mutagenized with NTG to derive thialysine(S-Lys)-resistant mutants. An AHV-resistant mutant, KY10484 was proved to be much more sensitive to the growth inhibition by thialysine than the parent strain, KY9159. From KY10484, a number of AHV- and thialysine-resistant mutants were derived. Approximately a half of these mutants were found to produce more l-threonine than KY10484. Among these mutants, KY10440 (Met−, AHVR, s-LysR) was used to investigate the cultural conditions for l-threonine production. The growth of KY10440 decreased largely with addition of l-homoserine, a threonine precursor. l-Asparagine, l-cystine, l-glutamine or l-arginine partially reversed the inhibitory effect of l-homoserine. Addition of these amino acids at low level led to increase l-threonine production. The amount of l-threonine accumulation reached to a level of 14mg/ml with a medium containing 10% glucose and to a level of 10 mg/ml with a medium containing 5% molasses (as glucose). Another AHV- and thialysine-resistant mutant, KY10251 which was also derived from KY9159 was found to produce both 9 mg/ml of l-threonine and 5.5 mg/ml of l-lysine in a culture broth.
Therapy of myasthenic crisis (1997)

None of the therapeutic regimens applied demonstrated any advantage over the others. All three regimens used were found to be effective and should be applied depending on the circumstances prevailing. Patients with myasthenic crisis must undergo careful cardiac monitoring, and temporary pace-making should be provided where clinically indicated.
Towards the Biological Understanding of CTC: Capture Technologies, Definitions and Potential to Create Metastasis (2013)

Circulating Tumor Cells (CTC) are rare cells originated from tumors that travel into the blood stream, extravasate to different organs of which only a small fraction will develop into metastasis. The presence of CTC enumerated with the CellSearch system is associated with a relative short survival and their continued presence after the first cycles of therapy indicates a futile therapy in patients with metastatic carcinomas. Detailed characterization of CTC holds the promise to enable the choice of the optimal therapy for the individual patients during the course of the disease. The phenotype, physical and biological properties are however not well understood making it difficult to assess the merit of recent technological advancements to improve upon the capture of CTC or to evaluate their metastatic potential. Here we will discuss the recent advances in the classification of CTC captured by the CellSearch system, the implications of their features and numbers. Latest capture platforms are reviewed and placed in the light of technology improvements needed to detect CTC. Physical properties, phenotype, viability and proliferative potential and means to assess their proliferation and metastatic capacity will be summarized and placed in the context of the latest CTC capture platforms.
Lenalidomide Oral Monotherapy Produces Durable Responses in Relapsed or Refractory Indolent Non-Hodgkin's Lymphoma (2009)

Oral lenalidomide monotherapy produces durable responses with manageable adverse events in patients with relapsed/refractory indolent NHL, warranting further investigation of treatment for indolent NHL.
Effects of various types of stress on the metabolism of reserve carbohydrates in Saccharomyces cerevisiae: genetic evidence for a stress-induced recycling of glycogen and trehalose (1997)

It is well known that glycogen and trehalose accumulate in yeast under nutrient starvation or entering into the stationary phase of growth, and that high levels of trehalose are found in heat-shocked cells. However, effects of various types of stress on trehalose, and especially on glycogen, are poorly documented. Taking into account that almost all genes encoding the enzymes involved in the metabolism of these two reserve carbohydrates contain between one and several copies of the stress-responsive element (STRE), an investigation was made of the possibility of a link between the potential transcriptional induction of these genes and the accumulation of glycogen and trehalose under different stress conditions. Using transcriptional fusions, it was found that all these genes were induced in a similar fashion, although to various extents, by temperature, osmotic and oxidative stresses. Experiments performed with an msn2/msn4 double mutant proved that the transcriptional induction of the genes encoding glycogen synthase (GSY2) and trehalose-6-phosphate synthase (TPS1) was needed for the small increase in glycogen and trehalose upon exposure to a mild heat stress and salt shock. However, the extent of transcriptional activation of these genes upon stresses in wild-type strains was not correlated with a proportional rise in either glycogen or trehalose. The major explanation for this lack of correlation comes from the fact that genes encoding the enzymes of the biosynthetic and of the biodegradative pathways were almost equally induced. Hence, trehalose and glycogen accumulated to much higher levels in cells lacking neutral trehalose or glycogen phosphorylase exposed to stress conditions, which suggested that one of the major effects of stress in yeast is to induce a wasteful expenditure of energy by increasing the recycling of these molecules. We also found that transcriptional induction of STRE-controlled genes was abolished at temperatures above 40 degree C, while induction was still observed for a heat-shock-element regulated gene. Remarkably, trehalose accumulated to very high levels under this condition. This can be explained by a stimulation of trehalose synthase and inhibition of trehalose by high temperature.
Cardiovascular pharmacology of bumepidil, a new synthetic vasoactive compound for coronary circulation. (1981)

Intravenously administered 8-tert.-butyl-7,8-dihydro-5-methyl-6H-pyrrolo[3,2-e]triazolo[1,5-alpyrimidine (bumepidil, CS-611) increased the coronary sinus outflow as well as intramyocardial flows in both subendocardial and subepicardial layers in the anesthetized dog and also the increase in the coronary arterial inflow was observed with short onset time, within 10 min when given orally to the conscious dog, and time for maximum effect is also short, i.e., 20 min after the oral administration. In the anesthetized rabbit flow-increase in the brain by CS-611 was next to the coronary, followed by the stomach and small intestines while there was no organ which showed a decreased flow. In the anesthetized open-chest dog cardiac output was increased by CS-611 while myocardial oxygen consumption was little affected. In addition, CS-611 exerted antiarrhythmic action on ventricular ectopics induced by coronary ligation of the conscious dog. A correlation between the increase in myocardial flow by CS-611 in the intact heart and the antiarrhythmic action in the coronary-lighted heart still remains to be studied further.
Solid-phase radioimmunoassay for IgG antibodies to Staphylococcus epidermidis. Use in serious coagulase-negative staphylococcal infections (1987)

• A radioimmunoassay (RIA) for human IgG antibodies to<i>Staphylococcus epidermidis</i>was compared with an agar-geldiffusion assay in patients with a variety of infections. The RIA was sensitive and reproducible and discriminated between endocarditis and uncomplicated bacteremias due to coagulase-negative staphylococci. Anti-<i>S epidermidis</i>antibodies by RIA were elevated in 16 (89%) of 18 patients with coagulase-negative staphylococcal endocarditis but in none of 28 patients with uncomplicated bacteremia (n =18) or with blood culture contaminated with these organisms (n =10). Cross-reacting IgG antibodies to<i>S epidermidis</i>antigens were also detected by RIA in 13 (76%) of 17 patients with<i>Staphylococcus aureus</i>endocarditis but in none of 17 patients with nonvalvular<i>S aureus</i>bacteremias and in none of 25 patients with endocarditis or bacteremia caused by other pathogens. Agar-geldiffusion assay was less sensitive than RIA for detecting coagulase-negative staphylococcal endocarditis, being positive in nine (50%) of 18 such patients. This RIA may be useful in distinguishing patients with endocarditis from those with nonvalvular staphylococcemias or blood culture contamination. (<i>Arch Intern Med</i>1987;147:689-693)
"Conjugal" transfer of plasmid DNA among oral streptococci. (1978)

The beta plasmid from Streptococcus faecalis strain DS5, which codes for resistance to erythromycin and lincomycin, was introduced into a Lancefield group F streptococcus, strain DR1501, by transformation. This strain, DR1501 (beta), was found to be an excellent donor of the beta plasmid and readily transferred the resistance markers to various lactic acid bacteria, including certain strains of S. mutans, S. sanguis, and S. salivarius. Evidence is presented indicating that the transfer of the beta plasmid is mediated by a mechanism that requires cell-to-cell contact. The transfer of plasmid DNA during conjugation has been confirmed by the isolation of beta plasmid from transconjugant clones and by their ability to then serve as donors of the erythromycin and lincomycin resistance markers.