gebhart/patrepeval huggingface dataset:
Assessment of cellular mass and lean body mass by noninvasive nuclear techniques. (1985)

With the nuclear techniques employed in this study, it has been possible to measure total body potassium (TBK), lean body mass (LBM), and body cell mass (BCM). The latter two are derived independently of TBK. LBM is derived from the sum of total body water (TBW), total body protein, and bone mineral ash measurements. The BCM value is, in turn, based on the difference between the LBM (as measured above) and the sum of the extracellular water and extracellular solids. It was demonstrated in 123 normal individuals that, although the TBK/LBM ratio decreases with age, the TBK/BCM ratio does not change significantly with age. It can be deduced that the ratio of TBK to intracellular water should be relatively constant with age. This relative constancy with age was demonstrated independently with the absolute measurements of TBK and intracellular water in normal individuals. Because the BCM is physiologically and chemically more homogeneous than LBM and because it reflects the actively metabolizing cellular compartment more accurately than LBM, it is the preferred parameter to be used for reference or normalization in body composition studies. For most applications, BCM is readily derived from TBK measurement by either whole body counting or isotope dilution techniques.
The Basic Science of Wound Healing (2006)

Understanding wound healing today involves much more than simply stating that there are three phases: "inflammation, proliferation, and maturation." Wound healing is a complex series of reactions and interactions among cells and "mediators." Each year, new mediators are discovered and our understanding of inflammatory mediators and cellular interactions grows. This article will attempt to provide a concise report of the current literature on wound healing by first reviewing the phases of wound healing followed by "the players" of wound healing: inflammatory mediators (cytokines, growth factors, proteases, eicosanoids, kinins, and more), nitric oxide, and the cellular elements. The discussion will end with a pictorial essay summarizing the wound-healing process.
High‐Molecular‐Weight Polynucleotides by Transferase‐Catalyzed Living Chain‐Growth Polycondensation (2017)

We present terminal deoxynucleotidyl transferase-catalyzed enzymatic polymerization (TcEP) for the template-free synthesis of high-molecular-weight, single-stranded DNA (ssDNA) and demonstrate that it proceeds by a living chain-growth polycondensation mechanism. We show that the molecular weight of the reaction products is nearly monodisperse, and can be manipulated by the feed ratio of nucleotide (monomer) to oligonucleotide (initiator), as typically observed for living polymerization reactions. Understanding the synthesis mechanism and the reaction kinetics enables the rational, template-free synthesis of ssDNA that can be used for a range of biomedical and nanotechnology applications.
Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation (2008)

These days it has been increasingly recognized that mast cells (MCs) are critical components of host defense against pathogens. In this study, we have provided the first evidence that MCs can kill bacteria by entrapping them in extracellular structures similar to the extracellular traps described for neutrophils (NETs). We took advantage of the ability of MCs to kill the human pathogen Streptococcus pyogenes by a phagocytosis-independent mechanism in order to characterize the extracellular antimicrobial activity of MCs. Close contact of bacteria and MCs was required for full antimicrobial activity. Immunofluorescence and electron microscopy revealed that S pyogenes was entrapped by extracellular structures produced by MCs (MCETs), which are composed of DNA, histones, tryptase, and the antimicrobial peptide LL-37. Disruption of MCETs significantly reduced the antimicrobial effect of MCs, suggesting that intact extracellular webs are critical for effective inhibition of bacterial growth. Similar to NETs, production of MCETs was mediated by a reactive oxygen species (ROS)-dependent cell death mechanism accompanied by disruption of the nuclear envelope, which can be induced after stimulation of MCs with phorbol-12-myristate-13-acetate (PMA), H(2)O(2), or bacterial pathogens. Our study provides the first experimental evidence of antimicrobial extracellular traps formation by an immune cell population other than neutrophils.
Complete Amino Acid Sequence of the Mu Heavy Chain of a Human IgM Immunoglobulin (1973)

The amino acid sequence of the micro, chain of a human IgM immunoglobulin, including the location of all disulfide bridges and oligosaccharides, has been determined. The homology of the constant regions of immunoglobulin micro, gamma, alpha, and epsilon heavy chains reveals evolutionary relationships and suggests that two genes code for each heavy chain.
A Message-Based Approach to Discrete-Event Simulation (1987)

This paper develops a message-based approach to discrete-event simulation. Although message-based simulators have the same expressive power as traditional discrete-event simulation lanuages, they provide a more natural environment for simulating distributed systems. In message-based simulations, a physical system is modeled by a set of message-communicating processes. The events in the system are modeled by message-communications. The paper proposes the entity construct to represent a message-communicating process operating in simulated time. A general wait until construct is used for process scheduling and message-communication. Based on these two notions, the paper proposes a language fragment comprising a small set of primitives. The language fragment can be implemented in any general-purpose, sequential programming language to construct a message-based simulator. We give an example of a message-based simulation language, called MAY, developed by implementing the language fragment in Fortran. MAY is in the public domain and is available on request.
The Use, Design, Synthesis, and Properties of High Performance/High Temperature Polymers: An Overview (2003)

An overview of the definition and development of, factors that contribute to, applications and markets for and the design of high performance/high temperature polymers is presented. Of the many families of high performance/high temperature polymers known, the most popular families consisting of polyimides, polyarylene ethers and phenylethynyl-terninated oligomers are used to demonstrate the basic principles in polymer development. Chemical structure/property relationships are used to show how polymers can be designed with a unique combination of properties. The estimated worldwide market for high temperature polymers in 2000 was 206,700,000 kgs constituting $4.36B with polyimides comprising 3,982,000 kgs or $1 .07B (24% of the dollar value). With an improvement in the world economy, this market is predicted to grow substantially
A Recombinant Human scFv Anti-Rh(D) Antibody With Multiple Valences Using a C-Terminal Fragment of C4-Binding Protein (1997)

Monomeric recombinant molecules prove generally unsatisfactory for in vivo use. Most biological systems are indeed multivalent either structurally, associating different chains, or functionally, when cross-linked by their ligands. Mimicking natural molecules for immune intervention implies the need for multimerizing systems to create multivalent molecules capable of interfering with physiological processing. A multivalent anti-Rh(D) recombinant protein has been designed by reconstructing the antibody binding site of a human monoclonal anti-Rh(D) antibody as a single chain Fv mini antibody, then multimerizing it by inserting at its C-terminal end the C-terminal part of the C4 binding protein (C4bp) alpha chain, which is responsible for the octamer multimerization of that molecule. This soluble multivalent recombinant molecule was functional, bound red blood cells (RBCs), agglutinated them, and did not activate complement. This demonstration model opens the way for future in vivo use of multivalent molecules associating antibody valences and other functional molecules for cell targeting, imaging, or removal of cells such as Rh(D)-positive RBCs for preventing Rh alloimmunization.
Is the Environment a Human Security Issue? (2008)

The concept of environmental security (ES) is rather new - at least as far as human security in the developing world is concerned. While the issue itself is not uncommon, its theoretical concepts and practical impact remain underinvestigated. This paper outlines the understanding of the concept, and provides a summary of the approaches, concerns and categories of ES. Various factors affecting environmental security are discussed and a number of arguments in favour of and against the concept evaluated before arriving at the conclusion that ES is an important component of human security.
8MM pulse impatt oscillators (2005)

The design principle of P/sup +/NN/sup +/ structure IMPATT pulse diode, fabrication technology, microwave circuit structure of oscillators and pulse modulators are presented in this paper. The frequency chirp effect during the bias pulse is explained and the way of reducing frequency chirp bandwidth is proposed.
In silico pharmacology for drug discovery: methods for virtual ligand screening and profiling (2007)

Pharmacology over the past 100 years has had a rich tradition of scientists with the ability to form qualitative or semi-quantitative relations between molecular structure and activity in cerebro. To test these hypotheses they have consistently used traditional pharmacology tools such as in vivo and in vitro models. Increasingly over the last decade however we have seen that computational (in silico) methods have been developed and applied to pharmacology hypothesis development and testing. These in silico methods include databases, quantitative structure-activity relationships, pharmacophores, homology models and other molecular modeling approaches, machine learning, data mining, network analysis tools and data analysis tools that use a computer. In silico methods are primarily used alongside the generation of in vitro data both to create the model and to test it. Such models have seen frequent use in the discovery and optimization of novel molecules with affinity to a target, the clarification of absorption, distribution, metabolism, excretion and toxicity properties as well as physicochemical characterization. The aim of this review is to illustrate some of the in silico methods for pharmacology that are used in drug discovery. Further applications of these methods to specific targets and their limitations will be discussed in the second accompanying part of this review.
Dynamics of bubble growth and detachment from a needle (1993)

Several aspects of the growth and departure of bubbles from a submerged needle are considered. A simple model shows the existence of two different growth regimes according to whether the gas flow rate into the bubble is smaller or greater than a critical value. These conclusions are refined by means of a boundary-integral potential-flow calculation that gives results in remarkable agreement with experiment. It is shown that bubbles growing in a liquid flowing parallel to the needle may detach with a considerably smaller radius than in a quiescent liquid. The study also demonstrates the critical role played by the gas flow resistance in the needle. A considerable control on the rate and size of bubble production can be achieved by a careful consideration of this parameter. The effect is particularly noticeable in the case of small bubbles, which are the most difficult ones to produce in practice.
Restoration of central nervous system α‐N‐acetylglucosaminidase activity and therapeutic benefits in mucopolysaccharidosis IIIB mice by a single intracisternal recombinant adeno‐associated viral type 2 vector delivery (2010)

A single IC rAAV2 gene delivery is functionally beneficial for treating the CNS disease of MPS IIIB in mice. It is immediately clinically translatable, with the potential of improving the quality of life for patients with MPS IIIB.
A Si-based FPW sensor array system with polymer microfluidics integrated on a PCB (2003)

Many methods for fabricating structures for microfluidic-based sensors have been developed in recent years. However, little has been reported on effective methods for integrating these structures into electronic systems for analysis and fluidic delivery. This paper describes a straightforward and versatile fabrication platform for polymer microfluidics that readily accommodates integration with silicon-based sensors, printed circuit, and surface mount technologies. In particular, we have demonstrated a novel system for distributed fluid delivery to a flexural plate wave (FPW) chemical/biological sensor where micromachined fluidic components are combined in a single package with silicon die, multilayer printed circuit board, and surface mount electronics. In the same fabrication platform, we have demonstrated temperature control of the sensor with 0.1/spl deg/C precision using integrated metal thin-film heater and sensor elements. This is an important capability for FPW sensors to compensate for temperature-induced drift. We present results for the on-board microfluidic system where the sensor is used to detect changes in the composition of the supplied fluid.
Preparation of novel heterocyclic systems from isatoic anhydrides (1979)

Abstract Isatoic anhydride ( 1a ) and 5‐chloroisatoic anhydride ( 1b ) were treated with 2‐(1‐methylhydrazino)ethanol ( 2 ) to produce 2‐aminobenzoic acid 2‐(2‐hydroxyethyl)‐2‐methylhydrazide ( 3a ) and its 5‐chloro analog 3b , respectively. Treatment of 3a and 3b with carbon disulfide gave, respectively, 2,3‐dihydro‐3‐[(2‐hydroxyethyl)methylamino]‐2‐thioxo‐4‐(1 H )quinazolinone ( 4a ) and its 6‐chloro analog 4b . Compounds 4a and 4b afforded 5,6‐dihydro‐5‐methyl‐2‐thioxo‐4 H ,8 H ‐[1,3,5,6]oxathiadiazocino[4,5‐ b ]quinazolin‐8‐one ( 5a ) and its 10‐chloro analog 5b , respectively, upon treatment with thiophosgene. Compound 5a could be produced directly from 3a and thiophosgene. Treatment of 4a and 4b with trifluoroacetic anhydride followed by potassium carbonate gave 3,4‐dihydro‐4‐methyl‐2 H ,6 H ‐[1,3,4]thiadiazino[2,3‐ b ]quinazolin‐6‐one ( 7a ) and its 8‐chloro analog 7b , respectively. Treatment of 4a with thionyl chloride also gave 7a , but 4b and thionyl chloride afforded a mixture of 7b and 8‐chloro‐3,4‐dihydro‐4‐methyl‐2 H ,6 H ‐[1,3,4]oxadiazino[2,3‐ b ]quinazolin‐6‐one ( 10 ). The dimethyl analogs of 4a and 4b ( 13a and 13b ) upon treatment with thiophosgene afforded 3,4‐dihydro‐2,2,4‐trimethyl‐2 H ,6 H ‐[1,3,4]oxadiazino[2,3‐ b ]quinazolin‐6‐one ( 14a ) and its 8‐chloro analog 14b , respectively.
Early discharge after mastectomy. A safe way of diminishing hospital costs. (1987)

In order to assess the risks and benefits of early discharge after major breast surgery, the authors analyzed 73 consecutive private patients who underwent operations by four surgeons over a 1-year period. Patient's ages ranged from 34 to 84 years, with a mean of 56.2 years. One patient was excluded from analysis because thoracotomy with a pulmonary resection was performed during the same hospitalization. Thirty-seven patients underwent total mastectomy with complete axillary dissection, 30 underwent segmental mastectomy with complete axillary dissection, and five underwent total mastectomy alone. For each patient the chest wall and axilla were drained by means of one or two Jackson-Pratt (American Heyer Schulte Corp., Goleta, CA) closed suction drains. Prior to discharge, all patients were instructed in the proper technique of drain care and were directed to record the daily drainage. Patients were discharged when they were fully mobile, did not require injectable narcotics, and felt capable of taking care of the drains as outpatients. The length of postoperative stay ranged from 1 to 9 days (mean 2.9), with all but three patients being discharged by the fifth postoperative day. Patient acceptance of early discharge with drains was excellent. Drains were pulled on an outpatient basis, usually within 7 to 10 days after surgery. Complications were observed in twelve patients (18%), consisting of seromas (8 patients), cellulitis (2 patients), and minimal superficial skin necrosis (2 patients). All complications were managed easily on an outpatient basis. We conclude that early discharge with Jackson-Pratt drains remaining in place is safe, well tolerated by patients, and has tremendous potential for substantial cost savings.
Mechanisms of neocortical epileptogenesis in vitro (1982)

1. The cellular mechanisms underlying interictal epileptogenesis have been examined in an in vitro slice preparation of guinea pig neocortex. Penicillin or bicuculline was applied to the tissue, and intracellular recordings were obtained from neurons and glia. 2. Following convulsant application, stimulation could elicit a short-latency excitatory postsynaptic potential (EPSP) and a large, longer latency depolarization shift (DS) in single neurons. DSs in neurons of the slice were very similar to those evoked in neurons of neocortex in vivo in that they displayed an all-or-none character, large shifts in latency during repetitive stimuli, long afterpotentials, and a prolonged refractory period. In contrast to epileptogenesis produced by penicillin in intact cortex, neither spontaneous DSs nor ictal episodes were observed in neocortical slices. 3. In simultaneous recordings from pairs of neurons within the same cortical column, DS generation and latency shifts were invariably synchronous. DS generation in neurons was also coincident with large, paroxysmal increases of extracellular [K+], as indicated by simultaneous recordings from glia. 4. When polarizing currents were applied to neurons injected with the local anesthetic QX-314, the DS amplitude varied monotonically and had an extrapolated reversal potential near 0 mV. In neurons injected with the K+-current blocker Cs+, large displacements of membrane potential were possible, and both the short-latency EPSP and the peak of the DS diminished completely at about 0 mV. At potentials positive to this, the short-latency EPSP was reversed, and the DS was replaced by a paroxysmal hyperpolarization whose rise time and peak latency were prolonged compared to the DS evoked at resting potential. The paroxysmal hyperpolarization probably represents the prolonged activation of the impaled neuron by EPSPs. 5. Voltage-dependent components, including slow spikes, appeared to contribute to generation of the DS at resting potential in Cs+-filled cells, and these components were blocked during large depolarizations. 6. The results suggest that DS generation in single neocortical neurons occurs during synchronous synaptic activation of a large group of cells. DS onset in a given neuron is determined by the timing of a variable-latency excitatory input that differs from the short-latency EPSP. The DS slow envelope appears to be generated by long-duration excitatory synaptic currents and may be modulated by intrinsic voltage-dependent membrane conductances. 7. We present a hypothesis for the initiation of the DS, based on the anatomical and physiological organization of the intrinsic neocortical circuits.
Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica (2017)

Yarrowia lipolytica was used to express fungal genes encoding hydrolytic enzymes of interest. We developed a successful droplet-based microfluidic platform for the high-throughput screening (10<sup>5</sup> strains/h) of Y. lipolytica based on enzyme secretion and activity. This approach provides highly efficient tools for the HTS of recombinant enzymatic activities. This should be extremely useful for discovering new biocatalysts via directed evolution or protein engineering approaches and should lead to major advances in microbial cell factory development.
Shortened telomeres in clonally expanded CD28-CD8+ T cells imply a replicative history that is distinct from their CD28+CD8+ counterparts (1996)

Long term in vitro culture of clonally expanded CD8+T cells, generally found within the CD57+ or CD28-subset, has generally been unsuccessful, suggesting that these cells may have a limited replicative potential. Telomeric shortening may reflect the action of a "mitotic clock" regulating the number of divisions a cell can undergo. In this study, we have compared the telomeric lengths of CD28-CD8+ and CD28+CD8+ T cells in 10 normal individuals to assess their replicative history. Overall, the telomeric lengths were found to be significantly shorter in the CD28-CD8+ T cell subset compared with the CD28+CD8+ subset. Furthermore, clonally expanded TCRBV11+CD8+ T cells from an individual exhibited telomeric lengths that were 2.9 kb shorter than those found in the polyclonal CD28+CD8+ T cell subset. These findings indicate that clonally expanded CD28-CD8+ T cells have undergone many more rounds of replication than CD28+CD8+ T cells, and consistent with the loss of CD28 expression, they may have reached a state of replicative senescence.
Expression of Plasmodium falciparum Circumsporozoite Proteins in Escherichia coli for Potential Use in a Human Malaria Vaccine (1985)

The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficiently expressed in the bacterium Escherichia coli. When injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic CS protein on live sporozoites and blocked sporozoite invasion of human hepatoma cells in vitro. These CS protein derivatives are therefore candidates for a human malaria vaccine.
Wireless Approach for SIP and SOC Testing (2010)

Jusqu'à présent, le test de circuits intégrés et des systèmes au niveau wafer est basé sur un contact physique entre l'équipement de test et les circuits sur le wafer. Cette méthode basée sur le contact est limitée par plusieurs facteurs, tels que le nombre de circuits testés en parallèle, la réduction de la taille et de l'espacement entre les plots de contact, le nombre de contact avant que les plots soient endommagés, le coût des opérations de test, entre autres. Pour résoudre ces problèmes, nous proposons une nouvelle approche de test basée sur la communication sans fil entre le testeur et les circuits à tester (DUT). Pour cela, un Wireless Test Control Bloc (WTCB) est ajouté à chaque DUT sur le wafer comme une interface sans fil entre le testeur et les structures de test internes du DUT. Ce WTCB intègre une pile protocolaire de communication pour gérer la communication avec le testeur, et un Test Control Bloc (TCB) pour gérer l'application de test au niveau DUT. Profitant d'une transmission sans fil, le testeur peut diffuser les données de test à tous les DUT sur le wafer , maximisant le test simultané et réduisant donc le temps de test. En outre, notre architecture de WTCB permet une comparaison locale de la réponse de DUT avec la réponse correcte attendue par le testeur. En effectuant cette comparaison dans le WTCB du DUT, le testeur recueille de chaque DUT 1 seul bit comme résultat de la comparaison, au lieu d'une réponse complète, conduisant à un test sans fil plus rapide qui réduit le temps d'essai. Le WTCB a été mis en oeuvre sur FPGA, et une épreuve de test sans fil d'un circuit réel a été réalisée, prouvant la conception efficace de notre WTCB, et soulignant le potentiel de notre méthode de test sans fil, où elle peut être étendue et utilisée pour des applications de test in situ à distance.