gebhart/patrepeval huggingface dataset:
Aggregation Analysis of the Microtubule Binding Domain in Tau Protein by Spectroscopic Methods (2003)

The microtubule-associated protein tau is a highly soluble protein that shows hardly any tendency to assemble under physiological conditions. In the brains of Alzheimer's disease (AD) patients, however, tau dissociates from the axonal microtubule and abnormally aggregates to form paired helical filaments (PHFs). One of the priorities in Alzheimer research is to clarify the mechanism of PHF formation. In recent years, several factors regulating tau assembly have come to light, yet some important questions remain to be answered. In this work, the His-tagged gene constructs of the four-repeat microtubule binding domain (4RMBD) in tau protein and its three mutants, 4RMBD S305N, N279K, and P301L, were expressed in E. coli and purified. Gel filtration chromatography and dynamic light scattering measurement yielded a Stokes radius of 3.1 nm, indicating that the His-tagged 4RMBD normally exists in buffer solution in a dimer state, which is formed by non-covalent intermolecular interactions. This non-covalent dimer can further polymerize to form filaments in the presence of polyanions such as heparin. The kinetics of the in vitro aggregation was monitored by thioflavine S dye fluorescence and CD measurements. The aggregation of 4RMBD was suggested to be a nucleation-dependent process, where the non-covalent dimer acts as an effective structural unit. The aggregation rate was strongly affected by the point mutation. Among the 4RMBD mutants, the rate of S305N was exceptionally fast, whereas N279K was the slowest, even slower than the wild-type. The aggregations were optimal in a weakly reducing environment for all the mutants and the wild type. However, the aggregations were affected differently by buffer pH, depending on the 4RMBD mutation.
α-ARYL-β-AROYLPROPIONIC ACIDS AND THEIR CONDENSATION PRODUCTS WITH AROMATIC ALDEHYDES (1956)

It has been shown, by means of spectral analysis, that α-aryl-β-aroylpropionic acids exist in an open-chain configuration while the condensation products of these latter acids with aromatic aldehydes are lactols. The mesityl derivatives constitute exceptions in both series. These findings necessitate a revision of some of the earlier literature.
Interaction of sound waves. Part IV: Scattering of sound by sound (1989)

A theory for the nonlinear interaction between two sound beams produced by real sources in a lossless fluid was presented in a previous work [Naze Tjo/tta and Tjo/tta, J. Acoust. Soc. Am. 83, 487–495 (1988)]. A general solution of the governing equation in the quasilinear approximation, valid at any range, crossing angle, and frequency ratio, was obtained for prescribed boundary conditions. An asymptotic formula for the sum and difference frequency sound pressure was obtained at large distance from the sources. It relates the amplitude and directivity of the sound field in the farfield to the on-source conditions. In the present work, the theory is further developed, and numerical results are presented for uniform piston and Gaussian sources. The influence of source geometry (separation, and intersection angles from 0° to 90°) and frequency on the beam pattern of the nonlinearly generated sound is studied. The results obtained are related to earlier works on scattering of sound by sound, which are discussed. Necessary conditions are also given to determine when scattering of sound by sound can be observed. In the special case of thin Gaussian beams intersecting at a small angle, the results are compared with that obtained by Darvennes and Hamilton [submitted to J. Acoust. Soc. Am.] using the paraxial approximation. Also discussed are some properties of the automatic global adaptive quadrature routine used to compute the two-dimensional integral in the asymptotic formula.
Isolation and characterization of human heparin (1992)

Heparin was isolated from an unusually large human hemangioma that contained an elevated level of mast cells. Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its structure and activity. Characterization of this human heparin included the following: one-dimensional and two-dimensional 1H-nuclear magnetic resonance spectral analysis; oligosaccharide mapping; saccharide compositional analysis; and in vitro assessment and anticoagulant activity. Oligosaccharide mapping and nuclear magnetic resonance spectroscopy showed that this human heparin is structurally similar to porcine intestinal mucosal heparin but distinctly different from bovine lung heparin. This human heparin also has substantially more in vitro anticoagulant activity than either of these pharmaceutical heparins.
Fuzzy network profiling for intrusion detection (2002)

The Fuzzy Intrusion Recognition Engine (FIRE) is an anomaly-based intrusion detection system that uses fuzzy logic to assess whether malicious activity is taking place on a network. It uses simple data mining techniques to process the network input data and help expose metrics that are particularly significant to anomaly detection. These metrics are then evaluated as fuzzy sets. FIRE uses a fuzzy analysis engine to evaluate the fuzzy inputs and trigger alert levels for the security administrator. This paper describes the components in the FIRE architecture and explains their roles. Particular attention is given to explaining the benefits of data mining and how this can improve the meaningfulness of the fuzzy sets. Fuzzy rules are developed for some common intrusion detection scenarios. The results of tests with actual network data and actual malicious attacks are described. The FIRE IDS can detect a wide-range of common attack types.
MOPC-315 Murine Plasmacytoma as a Model Anticancer Screen for Human Multiple Myeloma<xref ref-type="fn" rid="FN2">2</xref> (1986)

The murine tumor MOPC-315 plasmacytoma was studied as a model for human multiple myeloma. Plasmacytoma cells (10(6)) were injected iv into BALB/c mice, and 14 days later a single ip dose of the anticancer agent to be tested was administered at a dose that would result in 10% toxicity within 30 days (LD10). Increases in life-span and cures resulting from the LD10 dose were the parameters assessed. The response of this MOPC-315 plasmacytoma model to a variety of anticancer agents demonstrated good correlation with clinically active agents. A number of investigational agents were found to be highly active and potential candidates for clinical phase II studies.
Transmission properties of slanted annular aperture arrays Giant energy deviation over sub-wavelength distance (2015)

This paper is devoted to the study of the transmission properties of Slanted Annular Aperture Arrays made in perfectly conducting metal. More precisely, we consider the transmission based on the excitation of the cutoff-less guided mode, namely the TEM mode. We numerically and analytically demonstrate some intrinsic properties of the structure showing a transmission coefficient of at least 50% of an unpolarized incident beam independently of the illumination configuration (angle and plane of incidence). The central symmetry exhibited by the structure is analytically exploited to demonstrate the existence of a polarization state for which all the incident energy is transmitted through the sub-wavelength apertures when the eigenmode is excited, whatever are the illumination and the geometrical parameters. For this state of polarization, the laminar flow of the energy through the structure can exhibit giant deviation over very small distances. An example of energy flow deviation of 220° per wavelength is presented for illustration. The results presented in this paper could be considered as an important contribution to the understanding of the enhanced transmission phenomenon based on the excitation of guided modes.
On static compaction of test sequences for synchronous sequential circuits (1996)

Article Free Access Share on On static compaction of test sequences for synchronous sequential circuits Authors: Irith Pomeranz Electrical and Computer Engineering Department, University of Iowa, Iowa City, IA Electrical and Computer Engineering Department, University of Iowa, Iowa City, IAView Profile , Sudhakar M. Reddy Electrical and Computer Engineering Department, University of Iowa, Iowa City, IA Electrical and Computer Engineering Department, University of Iowa, Iowa City, IAView Profile Authors Info & Claims DAC '96: Proceedings of the 33rd annual Design Automation ConferenceJune 1996 Pages 215–220https://doi.org/10.1145/240518.240558Published:01 June 1996Publication History 114citation239DownloadsMetricsTotal Citations114Total Downloads239Last 12 Months7Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Piezoelectric Energy Harvesting Devices: An Alternative Energy Source for Wireless Sensors (2012)

The recent advances in ultralow power device integration, communication electronics, and microelectromechanical systems (MEMS) technology have fuelled the emerging technology of wireless sensor networks (WSNs). The spatial distributed nature of WSNs often requires that batteries power the individual sensor nodes. One of the major limitations on performance and lifetime of WSNs is the limited capacity of these finite power sources, which must be manually replaced when they are depleted. Moreover, the embedded nature of some of the sensors and hazardous sensing environment make battery replacement very difficult and costly. The process of harnessing and converting ambient energy sources into usable electrical energy is called energy harvesting. Energy harvesting raises the possibility of self-powered systems which are ubiquitous and truly autonomous, and without human intervention for energy replenishment. Among the ambient energy sources such as solar energy, heat, and wind, mechanical vibrations are an attractive ambient source mainly because they are widely available and are ideal for the use of piezoelectric materials, which have the ability to convert mechanical strain energy into electrical energy. This paper presents a concise review of piezoelectric microgenerators and nanogenerators as a renewable energy resource to power wireless sensors.
Replicative Senescence and Cell Immortality: The Role of Telomeres and Telomerase (1997)

Telomere shortening is correlated with cell senescence in vitro and cell aging in vivo. The telomere hypothesis suggests that telomere length serves as a mitotic clock for timing cellular replicative life span. Expression of telomerase stabilizes telomere length and allows for continual replication, or cell immortality. This article reviews recent evidences for the role of telomere length and telomerase in the regulation of cellular replicative life span. The therapeutic potential of manipulating telomerase expression and telomere length is also discussed.
A model for the hydrogen bond (1975)

A model of the linear hydrogen bond has been devised which is characterized by three physical parameters of the isolated electron donor and proton donor molecules: μ A-H , the bond dipole moment; Δ I , the difference in ionization potential between that of the electron donor and the corresponding noble gas atom; and l , the length of the electron donor lone-pair of electrons. The model is able to explain dimerization energy, charge transfer, internuclear separation, directionality, stretching force constants, dimer dipole moment, and infrared intensity enhancement.
Bacillus globigii Bugbeads:  A Model Simulant of a Bacterial Spore (2004)

Nonpathogenic microorganisms are often used as simulants of biological pathogens during the initial phase of detection method development. While these simulants approximate the size, shape, and cellular organization of the microorganism of interest, they do not resemble its surface protein content, a factor particularly important in methods based on immunorecognition. Here, we develop and detect an artificial bacterial spore--B. globigii (BG) Bugbead-a particle mimicking the antigenic surface of BG spores. Two methods of spore protein extraction were compared both quantitatively (by protein concentration assay) and qualitatively (by SDS-PAGE and Western blot): extraction by mechanical disruption and extraction by chemical decoating. The former method was more efficient in producing more protein and a greater number of antigens. BG Bugbeads were made by conjugating the extracted proteins to 0.8-microm carboxyl-coated polystyrene particles via carbodiimide coupling. BG Bugbeads were successfully detected by a bead-based enzyme-labeled immunoassay with fluorescence detection with a detection limit of 6.9 x 10(3) particles/mL. Formation of the Bugbead-capture bead complex was confirmed by ESEM. The concept of a harmless artificial spore can be applied to developing improved simulants for pathogenic spore-forming microorganisms such as B. anthracis, C. botulinum, and B. cereus, which can to be used for method validation, instrument calibration, and troubleshooting.
Measurement of the Distribution of Site Enhancements in Surface-Enhanced Raman Scattering (2008)

On nanotextured noble-metal surfaces, surface-enhanced Raman scattering (SERS) is observed, where Raman scattering is enhanced by a factor, G, that is frequently about one million, but underlying the factor G is a broad distribution of local enhancement factors, eta. We have measured this distribution for benzenethiolate molecules on a 330-nanometer silver-coated nanosphere lattice using incident light of wavelength 532 nanometers. A series of laser pulses with increasing electric fields burned away molecules at sites with progressively decreasing electromagnetic enhancement factors. The enhancement distribution P(eta)deta was found to be a power law proportional to (eta)(-1.75), with minimum and maximum values of 2.8 x 10(4) and 4.1 x 10(10), respectively. The hottest sites (eta >10(9)) account for just 63 in 1,000,000 of the total but contribute 24% to the overall SERS intensity.
HLA haplotype segregation in families with allergic asthma (1990)

In order to evaluate genetic linkage between Allergic Asthma (AA) and the HLA system, we studied 20 families having AA affected sib pairs and 8 families with Intrinsic Asthma (IA) affected sib pairs. All AA patients had a strong IgE immune response to the mite D. farinae. Serological HLA typing (A, B, C, DR, and DQ antigens) was performed by the standard microcytotoxicity assay. Genetic analysis was made by means of the "Affected sib pairs" method. Out of the 20 affected sib pairs, 14 shared two HLA haplotypes, five shared one HLA haplotype and one was HLA-non-identical. These results differed significantly from the random ratio 1:2:1 for sharing, 2, 1 or 0 haplotypes (p less than 0.0001), and was very close to that expected for a recessive mode of inheritance. In contrast, among the IA sib pairs there was not an important distortion in the pattern of haplotypes segregation. However, a significant association between any of the HLA alleles and the two types of Asthma studied was not found. The results suggest the existence of an HLA-linked recessive gene controlling the IgE immune responsiveness to mite allergens and conferring susceptibility to AA.
TNF-alpha expression in painful and nonpainful neuropathies (2001)

TNF-alpha expression of human Schwann cells may be up-regulated in painful neuropathies. The elevation of sTNF-RI in patients with centrally mediated mechanical allodynia suggests that systemic sTNF-RI levels may influence central pain processing mechanisms.
Recruitment of Brown Adipose Tissue as a Therapy for Obesity-Associated Diseases (2012)

Brown adipose tissue (BAT) has been recognized for more than 20 years to play a key role in cold-induced non-shivering thermogenesis (CIT, NST), and body weight homeostasis in animals. BAT is a flexible tissue that can be recruited by stimuli (including small molecules in animals), and atrophies in the absence of a stimulus. In fact, the contribution of BAT (and UCP1) to resting metabolic rate and healthy body weight homeostasis in animals (rodents) is now well established. Many investigations have shown that resistance to obesity and associated disorders in various rodent models is due to increased BAT mass and the number of brown adipocytes or UCP1 expression in various depots. The recent discovery of active BAT in adult humans has rekindled the notion that BAT is a therapeutic target for combating obesity-related metabolic disorders. In this review, we highlight investigations performed in rodents that support the contention that activation of BAT formation and/or function in obese individuals is therapeutically powerful. We also propose that enhancement of brown adipocyte functions in white adipose tissue (WAT) will also regulate energy balance as well as reduce insulin resistance in obesity-associated inflammation in WAT.
Fabrication of Y-gate, submicron gate length GaAs metal–semiconductor field effect transistors (1993)

0.1 μm Y-shape metal contacts for use as gates on GaAs metal–semiconductor field effect transistors (MESFETs) are produced using a novel SiNx deposition over features 1–2 μm wide, with subsequent etchback and gate metal deposition. Excellent across-wafer uniformity (≤10%) on 3 in. φ substrates is achieved, yielding a simple technique for extending the resolution of conventional optical lithography tools. One of the key features in this method is the need to have an initial negative resist profile. The SiNx is deposited at low temperature (50 °C) by plasma-enhanced chemical vapor deposition in order to avoid distortion of the resist, and exhibits conformal coverage over the initial resist openings. Submicron gate GaAs MESFETs fabricated by this technique have extrinsic transconductance, gm, value of ∼225 mS mm−1 at −0.5 V gate bias, and have comparable performance to devices fabricated using electron-beam lithography to produce a more conventional T-shape gate.
Kinetic Modeling of Amyloid Binding in Humans using PET Imaging and Pittsburgh Compound-B (2005)

A valid quantitative imaging method for the measurement of amyloid deposition in humans could improve Alzheimer's disease (AD) diagnosis and antiamyloid therapy assessment. Our group developed Pittsburgh Compound-B (PIB), an amyloid-binding radiotracer, for positron emission tomography (PET). The current study was aimed to further validate PIB PET through quantitative imaging (arterial input) and inclusion of subjects with mild cognitive impairment (MCI). Pittsburgh Compound-B studies were performed in five AD, five MCI, and five control subjects and five subjects were retested within 20 days. Magnetic resonance images were acquired for partial volume correction and region-of-interest definition (e.g., posterior cingulate: PCG; cerebellum: CER). Data were analyzed using compartmental and graphical approaches. Regional distribution volume (DV) values were normalized to the reference region (CER) to yield DV ratios (DVRs). Good agreement was observed between compartmental and Logan DVR values (e.g., PCG: r=0.89, slope=0.91); the Logan results were less variable. Nonspecific PIB retention was similar across subjects (n=15, Logan CER DV: 3.63+/-0.48). Greater retention was observed in AD cortical areas, relative to controls (P<0.05). The PIB retention in MCI subjects appeared either 'AD-like' or 'control-like'. The mean test/retest variation was approximately 6% in primary areas-of-interest. The Logan analysis was the method-of-choice for the PIB PET data as it proved stable, valid, and promising for future larger studies and voxel-based statistical analyses. This study also showed that it is feasible to perform quantitative PIB PET imaging studies that are needed to validate simpler methods for routine use across the AD disease spectrum.
Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle (2001)

Nuclear reprogramming requires the removal of epigenetic modifications imposed on the chromatin during cellular differentiation and division. The mammalian oocyte can reverse these alterations to a state of totipotency, allowing the production of viable cloned offspring from somatic cell nuclei. To determine whether nuclear reprogramming is complete in cloned animals, we assessed the telomerase activity and telomere length status in cloned embryos, fetuses, and newborn offspring derived from somatic cell nuclear transfer. In this report, we show that telomerase activity was significantly (P < 0.05) diminished in bovine fibroblast donor cells compared with embryonic stem-like cells, and surprisingly was 16-fold higher in fetal fibroblasts compared with adult fibroblasts (P < 0.05). Cell passaging and culture periods under serum starvation conditions significantly decreased telomerase activity by approximately 30-50% compared with nontreated early passage cells (P < 0.05). Telomere shortening was observed during in vitro culture of bovine fetal fibroblasts and in very late passages of embryonic stem-like cells. Reprogramming of telomerase activity was apparent by the blastocyst stage of postcloning embryonic development, and telomere lengths were longer (15-23 kb) in cloned fetuses and offspring than the relatively short mean terminal restriction fragment lengths (14-18 kb) observed in adult donor cells. Overall, telomere lengths of cloned fetuses and newborn calves ( approximately 20 kb) were not significantly different from those of age-matched control animals (P > 0.05). These results demonstrate that cloned embryos inherit genomic modifications acquired during the donor nuclei's in vivo and in vitro period but are subsequently reversed during development of the cloned animal.
Numerical analysis of a gas bubble near a rigid boundary in an oscillatory pressure field (1994)

The behavior of a gas bubble near a rigid boundary in an oscillatory pressure field is numerically investigated by means of two kinds of methods. The boundary integral method is utilized to simulate the bubble profiles at various times, and the image theory is also applied for solving the differential equations describing the nonlinear oscillations and migrations of a spherical gas bubble. Both calculations are performed, assuming that the liquid around a bubble is inviscid, incompressible, and irrotational and that the gas inside the bubble follows a polytropic gas law. The influence of the boundary proximity on the bubble motion is characterized by the bubble migration and the deformation of the bubble shape, especially by the liquid microjet formation. It is found that the period and the amplitude of the bubble oscillation are related not only to the nature of the time-dependent pressure in the liquid but also to the bubble location from a rigid wall. Furthermore, the influence of the boundary proximity on the frequency response curve associated with the bubble oscillation is examined on the supposition that the surrounding pressure oscillates with small amplitude and a rigid boundary is relatively far from a bubble.