gebhart/patrepeval huggingface dataset:
Synthesis of Chiral Benzimidazole‐Pyrrolidine Derivatives and their Application in Organocatalytic Aldol and Michael Addition Reactions (2007)

Abstract An efficient and mild approach for the synthesis of pyrrolidine‐benzimidazoles has been reported. They were further employed for the aldol and Michael addition reactions to afford the corresponding products with good yields and moderate enantioselectivities. Keywords: asymmetric aldolbenzimidazoleMichael additionorganocatalysispyrrolidine Acknowledgment We thank the CSIR for financial support under the Task Force Project CMM‐0005. G. Gopi Krishna and C. V. Rajasekhar thank CSIR, India, for the research fellowships.
Strong Optical Limiting of Silver-Containing Nanocrystalline Particles in Stable Suspensions (1998)

Metal and metal sulfide nanoparticles are prepared using a method that is based on the rapid expansion of supercritical fluid solution (RESS) into a liquid solution and characterized using transmission electron microscopy and X-ray diffraction methods. The nanoparticles form solution-like stable suspensions in the presence of a stabilization agent such as poly(N-vinyl-2-pyrrolidone) (PVP) polymer. The stable suspensions allow systematic nonlinear optical measurements. The nanocrystalline silver metal and silver sulfide particles in PVP polymer-stabilized ethanol suspensions of high linear transmittance exhibit excellent optical limiting properties, with the optical limiting responses toward nanosecond laser pulses at 532 nm being much stronger than those of benchmark materials [60]fullerene and chloroaluminum phthalocyanine in solution. A comparison of the results with those of stable suspensions of other nanoparticles including cadmium sulfide, lead sulfide, and nickel suggests that the optical limiting properties are unique to the nanoscopic silver-containing materials. Mechanistic issues concerning the optical limiting performance of the silver-containing nanocrystalline particles are discussed, and a nonlinear absorption mechanism is proposed.
Psychobiotics and the gut&ndash;brain axis: in the pursuit of happiness (2015)

The human intestine houses an astounding number and species of microorganisms, estimated at more than 10(14) gut microbiota and composed of over a thousand species. An individual's profile of microbiota is continually influenced by a variety of factors including but not limited to genetics, age, sex, diet, and lifestyle. Although each person's microbial profile is distinct, the relative abundance and distribution of bacterial species is similar among healthy individuals, aiding in the maintenance of one's overall health. Consequently, the ability of gut microbiota to bidirectionally communicate with the brain, known as the gut-brain axis, in the modulation of human health is at the forefront of current research. At a basic level, the gut microbiota interacts with the human host in a mutualistic relationship - the host intestine provides the bacteria with an environment to grow and the bacterium aids in governing homeostasis within the host. Therefore, it is reasonable to think that the lack of healthy gut microbiota may also lead to a deterioration of these relationships and ultimately disease. Indeed, a dysfunction in the gut-brain axis has been elucidated by a multitude of studies linked to neuropsychological, metabolic, and gastrointestinal disorders. For instance, altered microbiota has been linked to neuropsychological disorders including depression and autism spectrum disorder, metabolic disorders such as obesity, and gastrointestinal disorders including inflammatory bowel disease and irritable bowel syndrome. Fortunately, studies have also indicated that gut microbiota may be modulated with the use of probiotics, antibiotics, and fecal microbiota transplants as a prospect for therapy in microbiota-associated diseases. This modulation of gut microbiota is currently a growing area of research as it just might hold the key to treatment.
Cloning and analysis of s-triazine catabolic genes from Pseudomonas sp. strain NRRLB-12227 (1991)

Pseudomonas sp. strain NRRLB-12227 degrades the s-triazine melamine by a six-step pathway which allows it to use melamine and pathway intermediates as nitrogen sources. With the plasmid pLG221, mutants defective in five of the six steps of the pathway were generated. Tn5-containing-EcoRI fragments from these mutants were cloned and identified by selection for Tn5-encoded kanamycin resistance in transformants. A restriction fragment from ammelide-negative mutant RE411 was used as a probe in colony hybridization experiments to identify cloned wild-type s-triazine catabolic genes encoding ammeline aminohydrolase, ammelide aminohydrolase, and cyanuric acid amidohydrolase. These genes were cloned from total cellular DNA on several similar, but not identical, HindIII fragments, as well as on a PstI fragment and a BglII fragment. Restriction mapping and Southern hybridization analyses of these cloned DNA fragments suggested that these s-triazine catabolic genes may be located on a transposable element, the ends of which are identical 2.2-kb insertion sequences.
Epalons: Synthesis of 3α,7α-Dihydroxy-5α-pregnan-20-one (1998)

3α,7α-Dihydroxy-5α-pregnan-20-one ( 12 ) was prepared from (20 R )-pregn-5-ene-3β,20-diyl 3-acetate 20-benzoate ( 1 ). ∆ 5 -Olefin was oxidized in an allylic position and hydrogenated. Inversion of a configuration at carbon C-3 was carried out by solvolysis in N , N -dimethylformamide of 3β-tosylate in the presence of sodium nitrite. For stereoselective reduction of the 7-oxo group, L-Selectride® was used.
Regulation of nitric oxide synthesis in the liver (2000)

Nitric oxide signalling during the past two decades has been one of the most rapidly growing areas in biology. This simple free radical gas can regulate an ever-growing list of biological processes. Here the regulation of NO synthesis in the liver is reviewed. The biogenesis of nitric oxide (NO) is catalysed by nitric oxide synthases (NOS). These enzymes catalyse the oxidation of one of the guanidino nitrogens of l-arginine by molecular oxygen to form NO and citrulline. Three NOS have been identified: two constitutive (cNOS: type 1 or neuronal and type 3 or endothelial) and one inducible (iNOS: type 2). As to the liver, cNOS activity is normally detectable in Kupffer cells, whereas no cNOS is ever encoded in hepatocytes. However, hepatocytes, Kupffer and stellate cells (the three main types of liver cells) are prompted to express an intense iNOS activity once exposed to effective stimuli such as bacterial lipopolysaccharide and cytokines. This review is focused mainly on two aspects: regulation of NOS activity and expression by endogenous and exogenous compounds. Because NO production has beneficial and detrimental effects, understanding the molecular mechanisms that govern NOS is critical to developing strategies to manipulate NO production in liver diseases.
Adolescent suicidal risk assessment in clinician-patient interaction: A study of verbal and acoustic behaviors (2014)

Suicide among adolescents is a major public health problem: it is the third leading cause of death in the US for ages 13-18. Up to now, there is no objective ways to assess the suicidal risk, i.e. whether a patient is non-suicidal, suicidal re-attempter (i.e. repeater) or suicidal non-repeater (i.e. individuals with one suicide attempt or showing signs of suicidal gestures or ideation). Therefore, features of the conversation including verbal information and nonverbal acoustic information were investigated from 60 audio-recorded interviews of 30 suicidal (13 repeaters and 17 non-repeaters) and 30 non-suicidal adolescents interviewed by a social worker. The interaction between clinician and patients was statistically analyzed to reveal differences between suicidal vs. non-suicidal adolescents and to investigate suicidal repeaters' behaviors in comparison to suicidal non-repeaters. By using a hierarchical ensemble classifier we were able to successfully discriminate non-suicidal patients, suicidal repeaters and suicidal non-repeaters.
Multi-step organic synthesis using solid-supported reagents and scavengers: a new paradigm in chemical library generation (2000)

Covering: the literature until April 2000
Toward Animal Cell Culture–Based Influenza Vaccine Design: Viral Hemagglutinin N-Glycosylation Markedly Impacts Immunogenicity (2012)

The glycoproteins hemagglutinin (HA) and neuraminidase are the major determinants of host range and tissue tropism of the influenza virus. HA is the most abundant protein in the virus particle membrane and represents the basis of most influenza vaccines. It has been reported that influenza virus HA N-glycosylation markedly depends on the host cell line used for virus production. However, little is known about how differential glycosylation affects immunogenicity of the viral proteins. This is of importance for virus propagation in chicken eggs as well as for innovative influenza vaccine production in mammalian cell lines. In this study, we investigated the impact of the differential N-glycosylation patterns of two influenza A virus PR/8/34 (H1N1) variants on immunogenicity. Madin-Darby canine kidney cell-derived and Vero cell-derived glycovariants were analyzed for immunogenicity in a TCR-HA transgenic mouse model. Next-generation pyrosequencing validated the congruence of the potential HA N-glycosylation sites as well as the presence of the HA peptide recognized by the TCR-HA transgenic T cells. We show that differential HA N-glycosylation markedly affected T cell activation and cytokine production in vitro and moderately influenced IL-2 production in vivo. Cocultivation assays indicated that the difference in immunogenicity was mediated by CD11c(+) dendritic cells. Native virus deglycosylation by endo- and exoglycosidases dramatically reduced cytokine production by splenocytes in vitro and markedly decreased HA-specific Ab production in vivo. In conclusion, this study indicates a crucial importance of HA N-glycosylation for immunogenicity. Our findings have implications for cell line-based influenza vaccine design.
Radiobiological characterization of two human chemotherapy-resistant intermediate grade non-Hodgkin's lymphoma cell lines (1999)

Intermediate grade non-Hodgkin's lymphoma (IGNHL) is generally considered a radiosensitive tumor that can be controlled with moderate radiation doses. Cell-survival curves of cell lines derived from IGNHL have been typically described to exhibit small or no shoulder, implying inability to accumulate or repair sublethal radiation damage. We characterize in this report the clonogenic radiation survival curves of two human IGNHL cell lines, WSU-DLCL2 and SK-DHL2B, established from patients who expired after having exhibited chemotherapy resistance of their tumors. The cells were irradiated with 60Co radiation at a dose rate of 85-100 cGy/min and cell survival data were analyzed according to the linear quadratic model. The alpha/beta values for WSU-DLCL2 and SK-DHL2B cells are 2 and 8.6, respectively. The corresponding SF2 are 0.42 and 0.35, respectively. Both cell lines are able to repair radiation-induced sublethal damage. These data indicate that these cells are only moderately radiosensitive.
Effect of nutritional and enzymatic methionine deprivation upon human normal and malignant cells in tissue culture. (1980)

Human embryonic lung fibroblasts (F-136-35-56) capable of growing in medium containing DL-homocysteine instead of L-methionine and human acute lymphoblastic leukemia cells (CCRF-HSB-2) with absolute methionine requirement exhibited dose-dependent growth inhibition when semipurified L-methionine-degrading enzyme (L-methioninase, EC 4.4.1.11) was added to the tissue cultures. When D-homocystine was added to the cultures together with L-methioninase (0.1 units/ml, which completely degraded the available L-methionine in tissue culture), the F-136-35-56 cells continued to grow whereas the CCRF-HSB-2 cells were completely inhibited. In mixed cultures of the two cell lines with added L-methioninase + D-homocystine or L-methioninase + L-homocysteine thiolactone, the normal fibroblasts grew and synthesized DNA vigorously, whereas the lymphocytic malignant cells lost their viability completely and died within 3 to 4 days.
Distribution and regulation of plasminogen activator inhibitor-1 in murine adipose tissue in vivo. Induction by tumor necrosis factor-alpha and lipopolysaccharide. (1996)

Although elevated plasma plasminogen activator inhibitor 1 (PAI-1) is associated with obesity, very little is known about its tissue or cellular origin, or about the events that lead to increased PAI-1 levels under obese conditions. Since TNF-alpha is increased in rodents both during obesity and in response to endotoxin treatment, we examined the effects of these agents on PAI-1 gene expression in the adipose tissue of CB6 mice. In untreated mice, PAI-1 mRNA was detected in both mature adipocytes and in stromal vascular cells. Both TNF-alpha and endotoxin significantly increased PAI-1 mRNA in the adipose tissue, peaking at 3-8 h. In situ hybridization analysis of adipose tissue from untreated mice revealed a weak signal for PAI-1 mRNA only in the smooth muscle cells within the vascular wall. In contrast, after endotoxin or TNF-alpha treatment, PAI-1 mRNA also was detected in adipocytes and in adventitial cells of vessels. Endotoxin also induced PAI-1 in endothelial cells, while TNF-alpha additionally induced it in smooth muscle cells. Mature 3T3-L1 adipocytes in culture also expressed PAI-1 mRNA, and its rate of synthesis was also upregulated by TNF-alpha. These studies suggest that the adipose tissue itself may be an important contributor to the elevated PAI-1 levels observed in the plasma under obese conditions.
Engineering of immunogenic peptides by co‐linear synthesis of determinants recognized by B and T cells (1987)

The potential of synthetic peptides as vaccines is restricted by their frequent lack of immunogenicity. As with haptens, coupling to a carrier protein is usually required to provide T cell help to anti-peptide antibody-producing B cells. In spite of their short length, a few natural or synthetic peptides are immunogenic: they all include both a determinant recognized by B cells and a proven or putative determinant recognized by T cells. We speculated that it should be possible to induce immunogenicity in peptide haptens by the inclusion of a well characterized determinant recognized by T cells. We thus synthesized two peptides, corresponding to different regions of the major protein VP6 of bovine rotavirus, co-linearly linked to a peptide of influenza virus hemagglutinin which had been shown to induce T helper cells in BALB/c mice. Both peptides induced anti-rotavirus antibodies and were more immunogenic than the corresponding bovine serum albumin-conjugated peptides.
Augmented Lung Inflammation Protects against Influenza A Pneumonia (2009)

Together, these data indicate that innate immune resistance to influenza can be effectively stimulated, and suggest that ineffective rather than excessive inflammation is the major cause of mortality in influenza pneumonia.
ADOPTIVE IMMUNOTHERAPY OF EXPERIMENTAL OVARIAN-CANCER USING ACTIVATED HUMAN MONOCYTES AND THE HUMAN MONOCLONAL-ANTIBODY, ANTI-14C1 (1994)

We have examined the ability of the ovarian cancer cell-line OWmM1 to grow intraperitoneally in athymic ('nude') mice. The cell-line was tumorigenic and metastatic in a manner that paralleled the human disease; the metastatic nodules implanted onto the peritoneal surface, the diaphragm and the mesentery. The resulting tumour formed a clear ascitic fluid and peritoneal carcinomatosis. Adoptive transfer of activated human monocytes to the peritoneal cavity of tumour-bearing animals showed no increase in survival, while antibody alone produced a modest survival benefit (2-4 days). Administration of the human monoclonal antibody anti-14C1 and monocyte therapy together increased the benefit further and resulted in approximately 30% of the animals surviving until the end of the experiment (100 days).
Vertical and lateral GaN rectifiers on free-standing GaN substrates (2001)

Edge-terminated Schottky rectifiers fabricated on quasibulk GaN substrates showed a strong dependence of reverse breakdown voltage VB on contact dimension and on rectifier geometry (lateral versus vertical). For small diameter (75 μm) Schottky contacts, VB measured in the vertical geometry was ∼700 V, with an on-state resistance (RON) of 3 mΩ cm2, producing a figure-of-merit VB2/RON of 162.8 MW cm−2. Measured in the lateral geometry, these same rectifiers had VB of ∼250 V, RON of 1.7 mΩ cm2 and figure-of-merit 36.5 MW cm−2. The forward turn-on voltage (VF) was ∼1.8 V (defined at a current density of 100 A cm−2), producing VB/VF ratios of 139–389. In very large diameter (∼5 mm) rectifiers, VB dropped to ∼6 V, but forward currents up to 500 mA were obtained in dc measurements.
pH and the recycling of transferrin during receptor-mediated endocytosis. (1983)

At pH 5.4 apotransferrin (iron-free transferrin) binds to cell-surface transferrin receptors to the same extent and with the same affinity as does diferric transferrin at pH 7.0. Apotransferrin is quickly dissociated from its receptor when the pH is raised to 7.0. These and other results strongly support a simple model that explains the cycling of transferrin during a single cycle of receptor-mediated endocytosis. Diferric transferrin binds to cell-surface receptors, and the transferrin-receptor complex is endocytosed. The pH of the endocytic vesicle is lowered to 5.5 or below; this causes dissociation of iron from the transferrin-receptor complex, but apotransferrin remains bound to its receptor. The iron remains within the cell, and the apotransferrin-receptor complex is recycled to the cell surface. Upon encountering the neutral pH of the medium, apotransferrin is dissociated from the cell.
Multi-Face: Self-supervised Multiview Adaptation for Robust Face Clustering in Videos (2020)

Robust face clustering is a key step towards computational understanding of visual character portrayals in media. Face clustering for long-form content such as movies is challenging because of variations in appearance and lack of large-scale labeled video resources. However, local face tracking in videos can be used to mine samples belonging to same/different persons by examining the faces co-occurring in a video frame. In this work, we use this idea of self-supervision to harvest large amounts of weakly labeled face tracks in movies. We propose a nearest-neighbor search in the embedding space to mine hard examples from the face tracks followed by domain adaptation using multiview shared subspace learning. Our benchmarking on movie datasets demonstrate the robustness of multiview adaptation for face verification and clustering. We hope that the large-scale data resources developed in this work can further advance automatic character labeling in videos.
Life History and Arthropod Dispersal of a Coprophilous Stylopage (1991)

ABSTRACTStylopage anomala, previously known only from the type collection in England, is a member of an increasingly well known assemblage of dung fungi dispersed by arthropods rather than herbivore gut passage. CapiUiconidia of S. anomala were commonly found on phoretic gamasid mites of dung beetles collected in Ontario and New Brunswick, Canada, and also on a mite phoretic on a dipteran from Wyoming. Sappinia pedata, an amoeba preyed upon by S. anomala, has an adhesive stage that resists desiccation and can be dispersed by phoretic mites. Stylopage anomala and Sappinia pedata also have been found to occur together on cow and horse dung in Belgium.Key Words: ZoopagalesStylopageSappiniaamoebaearthropod dispersal
Sustained efficacy and tolerability in infants and young children with life-threatening hypophosphatasia treated with asfotase alfa (2014)

Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)
Sequential Hierarchical Scene Matching (1978)

The general approach to matching two scenes by a digital computer is usually costly in computations. A match is determined by selecting the position of maximum cross correlation between the window and each possible shift position of the search region. A new approach which is logarithmically efficient is presented in this paper. Its logarithmic efficiency and computational savings will be demonstrated both theoretically and in practical examples. Experimental results are presented for matching an image region corrupted by noise and for matching images from optical and radar sensors. The significance of this approach is that scene matching can be accomplished by the use of a computer even in cases which are difficult for humans or standard correlation techniques, and can be accomplished with greatly reduced computations.
Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay (2005)

A sensitive, simple, and instrument-independent method for the visual detection and identification of multiple nucleic acid amplicons by dipstick has been developed. This method is based on nucleic acid hybridization on the dipstick membrane and a signal amplification system to allow visual detection. With hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type 1 (HIV-1) as model analytes, it is demonstrated that the visual dipstick test combined with multiplex reverse transcription (RT)-PCR for the amplification of viral nucleic acid provides a specific and sensitive detection method. The RT-PCR products were detected by the dipstick with an efficiency similar to that of a complex, expensive, and instrument-dependent method based on fluorogenic oligonucleotide probes. The detection limits of the dipstick combined with multiplex RT-PCR were 50, 125, and 500 IU/ml for HBV DNA, HCV RNA, and HIV-1 RNA, respectively. The dipstick assay detected with similar efficiencies amplicons derived from strains of HBV genotypes A through F, HCV genotypes 1 to 6, and HIV-1 subtypes A through H as well as CRF02 circulating recombinant forms of HIV-1. Analysis of 295 clinical samples and 19 pools of 10 plasma specimens from blood donors revealed that multiplex dipstick detection was reproducible, sensitive, and specific. The visual dipstick detection of multiple amplicons thus provides an attractive alternative to complex, instrument-dependent detection methods currently in use for nucleic acid testing. This new and sensitive method for nucleic acid detection should increase the availability of genomic screening in resource-limited settings and its applicability to near-patient testing.
On the estimation of the coefficient of variation for anisotropic diffusion speckle filtering (2006)

In this paper, we focus on the problem of speckle removal by means of anisotropic diffusion and, specifically, on the importance of the correct estimation of the statistics involved. First, we derive an anisotropic diffusion filter that does not depend on a linear approximation of the speckle model assumed, which is the case of a previously reported filter, namely, SRAD. Then, we focus on the problem of estimation of the coefficient of variation of both signal and noise and of noise itself. Our experiments indicate that neighborhoods used for parameter estimation do not need to coincide with those used in the diffusion equations. Then, we show that, as long as the estimates are good enough, the filter proposed here and the SRAD perform fairly closely, a fact that emphasizes the importance of the correct estimation of the coefficients of variation.
Gain-flattened Er/sup 3+/-doped fiber amplifier for a WDM signal in the 1.57-1.60-μm wavelength region (1997)

A gain-flattened Er/sup 3+/-doped silica-based fiber amplifier (EDFA) has been constructed for a 1.58-μm band WDM signal. This EDFA exhibits uniform amplification characteristics with a gain excursion of 0.9 dB for a four-channel WDM signal in the 1.57-1.60 μm wavelength region. The average signal gain and the noise figure for the WDM signal are 29.5 dB and less than 6.3 dB, respectively. The use of this EDFA in parallel with a 1.55-μm band EDFA will expand the WDM transmission wavelength region.