gebhart/patrepeval huggingface dataset:
Induction of DNA Replication by Transcription in the Region Upstream of the Human c-myc Gene in a Model Replication System (1996)

An important relationship between transcription and initiation of DNA replication in both eukaryotes and prokaryotes has been suggested. In an attempt to understand the molecular mechanism of this interaction, we examined whether transcription can induce DNA replication in vitro by constructing a system in which both replication and transcription were combined. Relaxed circular DNA possessing a replication initiation zone located upstream of the human c-myc gene and a T7 promoter near the P1 promoter of the gene was replicated in the presence of T7 RNA polymerase. In our model system, replication was carried out with the proteins required for simian virus 40 DNA replication. DNA synthesis, which was dependent on both T7 RNA polymerase and the replication proteins, was detected mainly in the promoter and upstream regions of the c-myc gene. Blocking RNA synthesis at the initial stage of the reaction severely reduced DNA synthesis, suggesting that RNA chain elongation is required to induce DNA synthesis. The results indicated that transcription can induce DNA replication in the upstream region of the transcribed gene, most likely by introducing negative supercoiling into the region, which results in unwinding of the DNA duplex.
Highly Sensitive Sequencing of the Sulfated Domains of Heparan Sulfate (1999)

The heparan sulfates (HS) are hypervariable linear polysaccharides that act as membrane co-receptors for growth factors, chemokines, and extracellular matrix proteins. In most instances, the molecular basis of protein recognition by HS is poorly understood. We have sequenced 75% of the sulfated domains (S-domains) of fibroblast HS, including all of the major ones. This analysis revealed tight coupling of N- and 2-O-sulfation and a low frequency but precise positioning of 6-O-sulfates, which are required functional groups for HS-mediated activation of the fibroblast growth factors. S-domain sequencing was conducted using a novel and highly sensitive method based on a new way of reading the sequence from high performance liquid chromatography separation profiles of metabolically labeled HS-saccharides following specific chemical and enzymatic scission. The implications of the patterns seen in the sulfated domains for better understanding of the synthesis and function of HS are discussed.
Reduction of risk for growers: methods for the development of disease‐resistant crops (1996)

Summary Systemic acquired resistance (SAR) appears to be a ubiquitous higher plant defence response. Resistance is dependent on the high‐level expression of SAR genes. Mutations, transgenes or chemicals that cause high‐level SAR gene expression also cause resistance to pathogens. Furthermore, when SAR is eliminated, through the reduction of salicylic acid accumulation or activity, generalized severe disease‐susceptibility occurs. Therefore, SAR appears to be an essential component of the defensive repertoire that ensures plant health in nature. Chemicals that activate SAR in the field, as well as crop varieties with constitutive SAR gene expression, will provide new solutions to disease problems for growers in the near future.
Enterohaemorrhagic Escherichia coli. (1997)

Enterohaemorrhagic Escherichia coli produces Verotoxins (Shiga-like toxins) and causes diarrhoea, haemorrhagic colitis and haemorrhagic uraemic syndrome. The mode of action of the Shiga toxin family has been well characterized in numerous studies which have demonstrated that the toxin has RNA N-glycosidase activity, which inhibits the binding of EF-1-dependent aminoacyl-tRNA to the 60S ribosomal subunit of eukaryotic cells, thus resulting in an inhibition of protein synthesis of the cells. In 1996, an unexpectedly large number of outbreaks of food poisoning due to E. coli O157:H7 occurred in Japan. In addition, many sporadic cases of E. coli O157:H7 were also reported and a total of about 8400 patients were recorded during that year. In most of these cases, the food vectors could not be identified. The possibility of developing a candidate vaccine is also discussed in this article.
Manufacture of Superfine Organic Fibers (1954)

Abstract : Through a new process recently designed at NRL, it is now feasible to produce submicron organic fibers from a variety of thermoplastic materials. By this method an adjustable extruder forces a hot thermoplastic melt through a row of fine orifices into high-velocity dual streams of heated gas, usually air. The nozzle design provides for immediate resumption of attenuation following breaks, which are inevitable at submicron dimensions. Fiber diameters are determined by four basic process variables: air and nozzle temperatures, air pressure, and polymer feed rate (or ram pressure), all of which may be controlled independently. Nylon, linear polyester, polytrifluorochloroethylene, silicone, polystyrene, and other fibers can be produced, but those polymers which possess low melt viscosities attenuate most readily. Materials such as polyvinyl chloride and polyacrylonitrile which do not melt or soften sufficiently below their decomposition temperature cannot be employed in this process. Proper balancing of all the variables yields fine fibers of good uniformity and quality. By eliminating intermediate operations of fiber chopping and wet techniques of paper making, preparation of fabrics from thermoplastics is promising economically as well as technologically.
2340 EBI-005: an Interleukin-1 Receptor Inhibitor Designed for the Treatment of Dry Eye Syndrome (2012)

Dry eye syndrome (DES) is an ocular surface inflammatory condition induced by desiccating stress and mediated by IL-1 stimulation of the Th17 pathway.1,2,3 DES affects approximately 10% of individuals between 30 to 60 years of age, and up to 15% of those over 65. Despite its prevalence and impact on quality of life, DES is under-diagnosed, under-treated and has few safe and effective treatment options.
Evaluation of the biocompatibility of calcium phosphate cement/PLGA microparticle composites (2008)

In this study, the biocompatibility of a calcium phosphate (CaP) cement incorporating poly (D,L-lactic-co-glycolic acid) (PLGA) microparticles was evaluated in a subcutaneous implantation model in rats. Short-term biocompatibility was assessed using pure CaP discs and CaP discs incorporating PLGA microparticles (20% w/w) with and without preincubation in water. Long-term biocompatibility was assessed using CaP discs incorporating varying amounts (5, 10, or 20% w/w) and diameter sizes (small, 0-50 mum; medium, 51-100 mum, or large, 101-200 mum) of PLGA microparticles. The short-term biocompatibility results showed a mild tissue response for all implant formulations, irrespective of disc preincubation, during the early implantation periods up to 12 days. Quantitative histological evaluation revealed that the different implant formulations induced the formation of similar fibrous tissue capsules and interfaces. The results concerning long-term biocompatibility showed that all implants were surrounded by a thin connective tissue capsule (<10 layers of fibroblasts). Additionally, no significant differences in capsule and interface scores were observed between the different implant formulations. The implants containing 20% PLGA with medium- and large-sized microparticles showed fibrous tissue ingrowth throughout the implants, indicating PLGA degradation and interconnectivity of the pores. The results demonstrate that CaP/PLGA composites evoke a minimal inflammatory response. The implants containing 20% PLGA with medium- and large-sized microparticles showed fibrous tissue ingrowth after 12- and 24-weeks indicating PLGA degradation and interconnectivity of the pores. Therefore, CaP/PLGA composites can be regarded as biocompatible biomaterials with potential for bone tissue engineering and advantageous possibilities of the microparticles regarding material porosity.
Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA. (1980)

A plasmid containing two cloned hepatitis B virus genomes in a tandem head-to-tail arrangement has been introduced into mouse fibroblasts by using cotransformation with the cloned herpes simplex virus thymidine kinase gene. Several copies of the plasmid were integrated into high molecular weight cellular DNA. The original tandem structure of the hepatitis B virus DNA was conserved. Hepatitis B surface antigen was synthesized by all the 15 clones examined. The other viral antigens were not detected. The surface antigen was excreted into the cell culture medium as particles having the same characteristics as those found in human serum. It is estimated that 2-4 X 10(4) particles were produced per mouse cell per 24 hr in two clones. This value corresponds to approximately 2-4 X 10(6) surface antigen polypeptides per cell per 24 hr.
Vasoactive Intestinal Peptide-Receptor Imaging for the Localization of Intestinal Adenocarcinomas and Endocrine Tumors (1994)

Scanning with radiolabeled VIP can visualize intestinal tumors and metastases that express receptors for VIP.
Bocavirus Infection Induces Mitochondrion-Mediated Apoptosis and Cell Cycle Arrest at G2/M Phase (2010)

Bocavirus is a newly classified genus of the family Parvovirinae. Infection with Bocavirus minute virus of canines (MVC) produces a strong cytopathic effect in permissive Walter Reed/3873D (WRD) canine cells. We have systematically characterized the MVC infection-produced cytopathic effect in WRD cells, namely, the cell death and cell cycle arrest, and carefully examined how MVC infection induces the cytopathic effect. We found that MVC infection induces an apoptotic cell death characterized by Bax translocalization to the mitochondrial outer membrane, disruption of the mitochondrial outer membrane potential, and caspase activation. Moreover, we observed that the activation of caspases occurred only when the MVC genome was replicating, suggesting that replication of the MVC genome induces apoptosis. MVC infection also induced a gradual cell cycle arrest from the S phase in early infection to the G(2)/M phase at a later stage, which was confirmed by the upregulation of cyclin B1 and phosphorylation of cdc2. Cell cycle arrest at the G(2)/M phase was reproduced by transfection of a nonreplicative NS1 knockout mutant of the MVC infectious clone, as well as by inoculation of UV-irradiated MVC. In contrast with other parvoviruses, only expression of the MVC proteins by transfection did not induce apoptosis or cell cycle arrest. Taken together, our results demonstrate that MVC infection induces a mitochondrion-mediated apoptosis that is dependent on the replication of the viral genome, and the MVC genome per se is able to arrest the cell cycle at the G(2)/M phase. Our results may shed light on the molecular pathogenesis of Bocavirus infection in general.
Schaum's outline of review of elementary mathematics (1997)

Fundamentals of Arithmetic: Number. Fundamentals of Arithmetic and Introduction to Calculators. Fractions. Decimals. Percents. Signed Numbers. Fundamentals of Algebra: Laws and Operations. Fundamentals of Algebra: Equations and Formulas. Ratios, Proportions, and Rates. Fundamentals of Geometry.
Front-end modules with versatile dynamic EVM correction for 802.11 applications in the 2GHz band (2014)

A SiGe BiCMOS, CMOS/SOI front-end module (FEM) for WLAN applications in the 2GHz band is presented. In the transmit mode it delivers 29dB small-signal gain and 3% EVM at 19.3dBm. In the receive mode, it achieves a noise figure of 1.85dB, 14dB gain, and an IIP3 level of 3dBm with 9mA current consumption. The FEM employs bias control circuitry that can be configured to address the linearity requirements of the WLAN standards family under varying environmental and channel conditions, including the emerging 802.11ac standard.
Study of separation force in constrained surface projection stereolithography (2017)

Purpose Recently, the constrained surface projection stereolithography (SL) technology is gaining wider attention and has been widely used in the 3D printing industry. In constrained surface projection SL systems, the separation of a newly cured layer from the constrained surface is a historical technical barrier. It greatly limits printable size, process reliability and print speed. Moreover, over-large separation force leads to adhesion failures in manufacturing processes, causing broken constrained surface and part defects. Against this background, this paper investigates the formation of separation forces and various factors that affect the separation process in constrained surface projection SL systems. Design/methodology/approach A bottom-up projection SL testbed, integrated with an in-situ separation force measurement unit, is developed for experimental study. Separation forces under various manufacturing process settings and constrained surface conditions are measured in situ . Additionally, physical models are constructed by considering the liquid resin filling process. Experiments are conducted to investigate influences of manufacturing process settings, constrained surface condition and print geometry on separation forces. Findings Separation forces increase linearly with the separation speed. The deformation and the oxygen inhibition layer near the constrained surface greatly reduce separation forces. The printing area, area/perimeter ratio and the degree of porousness of print geometries have a combined effect on determining separation forces. Originality/value This paper studied factors that influence separation force in constrained surface SL processes. Constrained surface conditions including oxygen inhibition layer thickness, deformation and oxygen permeation capability were investigated, and their influences on separation forces were revealed. Moreover, geometric factors of printing layers that are significant on determining separation forces have been identified and quantified. This study on separation forces provides a solid base for future work on adaptive control of constrained surface projection SL processes.
Ambiguity-free Doppler centroid estimation technique for airborne SAR using the Radon transform (2005)

In synthetic aperture radar (SAR) signal processing, the Doppler centroid estimation technique, called the "clutter-lock", is important because it is related to the signal-to-noise ratio, geometric distortion, and radiometric error of the final SAR image. Conventional algorithms have either ambiguity problems or somewhat high computational load. Using the fact that the Doppler centroid and the squint angle are directly related, we propose an ambiguity-free Doppler centroid estimation technique using Radon transform, named geometry-based Doppler estimator. The proposed algorithm is computationally efficient and shows good performance of estimating the absolute Doppler centroid.
<title>Automated prefetch mechanism: design and implementation for a radiology PACS</title> (1994)

A clinical picture archiving and communication system (PACS) requires immediate access to both patients' previous and current images as well as the corresponding radiology reports. We have developed a prefetch mechanism for the PACS system in the UCSF Department of Radiology that automatically retrieves historical images and reports for reviewing at remote display stations.
The secreted form of the epidermal growth factor receptor. Characterization and crystallization of the receptor-ligand complex. (1990)

A protein composed of the external domain of the epidermal growth factor (EGF) receptor is secreted by A431 human tumor cells. The soluble receptor protein was isolated in bulk quantities from cell culture supernatants. It has an intact ligand binding site, exists in a 93-kDa monomeric form, and does not undergo oligomerization upon ligand binding; thus the receptor dimerization reported for the EGF holoreceptor appears not to be a function of its external domain. The unique system of a physiological soluble receptor was utilized for a crystallization study. Crystals were obtained but only in the presence of the ligand. They contained (in equimolar amounts) receptor as well as EGF. The crystals belong to the tetragonal space group P4(3)2(1)2 or P4(1)2(1)2 with unit cell dimensions a = b = 118 A, c = 202 A. The packing density parameter was 3.55 A3/dalton, indicating the asymmetric unit to consist of one receptor-ligand complex.
Control of resonant wavelength from organic light-emitting materials by use of a Fabry-Perot microcavity structure (2002)

We report the fabrication of Fabry-Perot microcavity structures with the organic light-emitting material tris-(8-hydroxyquinoline) aluminum (Alq3) and derive their optical properties by measuring their photoluminescence (PL) and absorption. Silver and a TiO2-SiO2 multilayer were used as metal and dielectric reflectors, respectively, in a Fabry-Perot microcavity structure. Three types of microcavity were prepared: type A consisted of [air[Ag[Alq3]Ag]glass]; type B, of [air[dielectric[Alq3]dielectric]glass]; and type C, of [air[Ag[Alq2]dielectric]glass]. A bare Alq3 film of [air[Alq3]glass] had its PL peak near 514 nm, and its full width at half-maximum (FWHM) was 80 nm. The broad FWHM of a bare Alq3 film was reduced to 15-27.5, 7-10.5, and 16-16.6 nm for microcavity types A, B, and C, respectively. Also, we could control the PL peak of the microcavity structure by changing the spacer thickness, the amount of phase change on reflection, and the angle of incidence.
Trialkylphosphine-Mediated Synthesis of 2-Acyl Furans from Ynenones (2017)

A novel reaction for the synthesis of 2-acyl furans is reported. The reaction is believed to proceed by sequential addition of a trialkylphosphine to an ynenone, 5-exo-dig cyclization to form the furan, and oxidation of the resulting phosphonium ylide with molecular oxygen. Many common functional groups are tolerated during the reaction, and the products are obtained in good to excellent yield under the mild conditions. This methodology offers efficient access to biologically important compounds, including fused polycyclic compounds and furaldehydes, from simple starting materials.
Purification and functional characterization of integrin alpha v beta 5. An adhesion receptor for vitronectin. (1990)

We have purified a novel member of the integrin gene family from placenta that serves as a vitronectin receptor. This integrin is composed of the alpha v subunit and a beta subunit that we designate beta 5. Purification was accomplished by immunodepleting a placental extract of integrin alpha v beta 3, allowing us to purify alpha v beta 5 from the remaining extract by monoclonal antibody affinity chromatography on LM 142-Sepharose, which binds to the alpha v subunit. Purification to homogeneity was subsequently achieved by affinity chromatography on wheat germ lectin-Sepharose. Western blot analysis with antibodies raised against alpha v beta 5 and alpha v beta 3 demonstrated that beta 3 and beta 5 were distinct but confirmed that the alpha subunit of the two integrins were immunologically identical. Similarly, antibodies that bind beta 3 proximal to the ligand-binding site failed to react with beta 5, indicating an architectural difference at the ligand-binding site of these related integrins. This structural difference apparently results in a functional distinction, since purified alpha v beta 3 bound to vitronectin, fibrinogen, von Willebrand factor, and fibronectin, whereas integrin alpha v beta 5 bound preferentially to vitronectin. Finally, we demonstrate by three criteria that beta 5 and beta x, the latter of which was identified in lung carcinoma cells (Cheresh, D. A., Smith, J. W., Cooper, H. M., and Quaranta, V. (1989) Cell 57, 59-69), are identical. First, peptide maps of beta x and beta 5 are identical. Secondly, polyclonal antibodies raised against alpha v beta 5 immunoprecipitate both beta 5 and beta x, and finally, the amino-terminal amino acid sequences of beta x and beta 5 are identical.
Human Metapneumovirus: A New Player among Respiratory Viruses (2004)

The human metapneumovirus (hMPV) is a newly described member of the Paramyxoviridae family belonging to the Metapneumovirus genus. Since its initial description in 2001, hMPV has been reported in most parts of the world and isolated from the respiratory tract of subjects from all age groups. Despite the fact that prospective and case-control studies have been limited, the epidemiology and clinical manifestations associated with hMPV have been found to be reminiscent of those of the human respiratory syncytial virus, with most severe respiratory tract infections occurring in infants, elderly subjects, and immunocompromised hosts. Additional research is needed to define the pathogenesis of this viral infection and the host's specific immune response.
On the spectral efficiency of CPM systems over real channel in the presence of adjacent channel and cochannel interference: a comparison between partial and full response systems (1990)

In all the new radio systems to be designed-for example, local radio networks or portable communication systems-a crucial point is determination of the actual spectral efficiency obtainable. To this end, the fundamental role played by adjacent and cochannel interference must be considered. The computer program implemented allows general analysis of continuous phase modulation (CPM) systems with limiter-discriminator detection and symbol-by-symbol regeneration, the combined effects of intersymbol interference, noise, and adjacent channel or cochannel interference have been tested to give the design criteria of the transmission system. To emphasize the role played by interference, an ideal multipath-free channel has been considered: with reference to a binary case, Gaussian minimum-shift keying (GMSK) systems have been investigated and compared with full-response CPM systems to obtain a suitable tradeoff between spectral efficiency (connected to channel spacing at radio frequency) and signal-to-noise ratio with fixed bit-error probability. The numerical results presented emphasize the performance obtainable with full- and partial-response techniques for varying system parameters such as phase deviation index, receiver filter bandwidth, and spectral efficiency.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cystatin M / E expression in inflammatory and neoplastic skin disorders (2002)

Inflammation causes cystatin M/E to be expressed in the spinous cell layers where it colocalizes with transglutaminase for which it serves as a substrate. Speculatively, increased expression of cystatin M/E is compatible with a role in controlling increased levels of cysteine proteinases during inflammation and infection. Cystatin M/E expression in neoplastic epidermis is confined to well-differentiated cells and as such does not discriminate between benign and (pre)malignant epidermal neoplasias.
Simplified Quantification of Myocardial Flow Reserve with flurpiridaz F 18: Validation with Microspheres in a Pig Model (2011)

Myocardial retention and SUVs of the (18)F-labeled flow tracer flurpiridaz F 18 accurately reflect the MFR. These simplified analysis methods may facilitate the combination of quantitative assessment of perfusion reserve and rapid clinical imaging protocols.
Bis-1,3-dithiolium chloride: an unusually stable organic radical cation (1970)

F. Wudl, G. M. Smith and E. J. Hufnagel, J. Chem. Soc. D, 1970, 1453 DOI: 10.1039/C29700001453
Metabolism of Hydralazine: Relevance to Drug-Induced Lupus (1994)

(1994). Metabolism of Hydralazine: Relevance to Drug-Induced Lupus. Drug Metabolism Reviews: Vol. 26, No. 3, pp. 485-505.