gebhart/patrepeval huggingface dataset:
Sequential study of myeloid differentiation antigens of neutrophil granulocytes in different phases of chronic myeloid leukaemia: natural history and prognostic significance. (1993)

In an attempt to contribute to the knowledge of the natural history of Philadelphia-chromosome-positive chronic myeloid leukaemia (CML) and its prognosis, we analyzed sequentially the myeloid differentiation antigens of peripheral blood neutrophil granulocytes (NG) in different evolutive stages of the disease. Four monoclonal antibodies (CD15, CD24, 31D8, and 13F6) were used, and a total number of 116 sequential studies were performed in 43 patients. At diagnosis, there is a significant decrease of NG expressing myeloid differentiation antigens, which recover to nearly normal levels after initial control of the disease. The onset reduction is probably due to the circulation of incompletely mature NG. In accelerated/blastic phase NG expressing myeloid differentiation antigens decrease again, probably due to a true antigen loss. This reduction could herald by a few months the development of accelerated/blastic phase. In such a case, its predictive strength is higher than that of the well recognized initial prognostic parameters in CML. These results indicate that the sequential study of NG myeloid differentiation antigens may contribute to both a better understanding of the natural history of CML and the evolutive prognosis of this disease.
Assessing the multimeric states of proteins: Studies using laser desorption mass spectrometry (1991)

We have developed a technique which utilizes matrix-assisted laser desorption mass spectrometry to study the subunit association of proteins. Aqueous protein samples are treated with a dilute solution of glutaraldehyde, a cross-linking agent which reacts with free amino groups on proteins. This agent effectively traps the multimeric form, preventing it from dissociating in the sample preparation and desorption process. Proteins measured include lysozyme, carbonic anhydrase, apomyoglobin, glucose 6-phosphate dehydrogenase, ovine lutropin, yeast alcohol dehydrogenase, avidin and pyruvate kinase. Dimeric and tetrameric complexes up to 250,000 Da have been measured in this manner.
Treatment and side effect management of CTLA‐4 antibody therapy in metastatic melanoma (2010)

Immune-modifying monoclonal antibodies may induce or enhance the natural immune response against tumor cells. The complex interaction between antigen-presenting cells and T lymphocytes as an immune response is strongly affected by anti-CD152 (CTLA-4)-antibodies. The cytotoxic T-lymphocyte (CTLA-4) receptor binds molecules of the B7-family which leads to a suppression of T cells. Specific CTLA-4 antibodies induce an unrestrained T-cell activation. Treatment with the CTLA-4 antibodies ipilimumab and tremelimumab has been investigated in metastatic melanoma only within clinical trials. Currently, the critical phase III trial on ipilimumab is in the final analysis process and expected to lead to approval. CTLA-4 antibodies belong to the most promising new molecules for the treatment of advanced melanoma. During treatment with CTLA-4 antibodies, distinct adverse events may occur. Treating physicians must be familiar with their appropriate treatment and prophylaxis. The most frequently observed side effects are diseases such as an autoimmune colitis which is typically characterized by a mild to moderate, but occasionally also severe and persistent diarrhea. Other autoimmune-mediated side effects like hypophysitis, hepatitis, iridocyclitis or an exacerbation of lupus nephritis have been reported in the literature. Their early recognition and treatment are mandatory to reduce the risk of sequelae for CTLA-4-antibod-treated patients. Autoimmune-mediated side effects are reported to correlate positively with treatment response. We review the mechanisms of action, provide an update on clinical trials with the two CTLA-4-antibodies for metastatic melanoma, and present detailed recommendations for managing the side effects of these new agents.
Purification and characterization of novel “2-arylpropionyl-CoA epimerases” from rat liver cytosol and mitochondria. (1993)

Investigation on the biochemical isomerization of ibuprofen led us to the successful purification of "2-arylpropionyl-CoA epimerase" from rat liver cytosol and mitochondria. The purified enzymes from both subcellular fractions exhibit similar physical and catalytic properties and are distinctly different from rat liver methylmalonyl-CoA epimerase. Both are monomeric proteins with an apparent molecular mass of 42 kDa and show similar contents of most amino acids. Their UV spectra gave no indication of any bound cofactors, and their enzyme activities were not affected by exposure to EDTA or metal ions (except Cu2+). These results suggest that the cytosolic and mitochondrial epimerases may be structurally related. The purified enzymes catalyze the epimerization of various 2-arylpropionyl-CoAs with some degree of stereochemical differentiation. For 2-(4-isobutylphenyl)propionyl-CoA, the equilibrium constant was estimated to be 1.5 in favor of the R-isomer. Evidence indicated that the proton exchange may be mediated by a 2-base mechanism and that a carboxylic residue in the active site may serve as a general base for proton abstraction.
Sparse Additive Models (2009)

Summary We present a new class of methods for high dimensional non-parametric regression and classification called sparse additive models. Our methods combine ideas from sparse linear modelling and additive non-parametric regression. We derive an algorithm for fitting the models that is practical and effective even when the number of covariates is larger than the sample size. Sparse additive models are essentially a functional version of the grouped lasso of Yuan and Lin. They are also closely related to the COSSO model of Lin and Zhang but decouple smoothing and sparsity, enabling the use of arbitrary non-parametric smoothers. We give an analysis of the theoretical properties of sparse additive models and present empirical results on synthetic and real data, showing that they can be effective in fitting sparse non-parametric models in high dimensional data.
PROLONGATION OF RAT KIDNEY ALLOGRAFTS BY PRETRANSPLANT ADMINISTRATION OF DONOR ANTIGEN EXTRACT OR WHOLE BLOOD TRANSFUSION COMBINED WITH A SHORT COURSE OF CYCLOSPORINE1 (1983)

The immunosuppressive effect of the combination of a three day course of cyclosporine with one i.v. injection of 3M KCL-extracted donor antigen or donor blood transfusion was tested across the strong histocompatibility barrier causing rejection within 8 days of kidney transplants from Buffalo (Buf, RT1b) to Wistar-Furth (WFu, RT1u) inbred rats. Administration of 10 mg/kg/day cyclosporine alone for three days (-1, 0, and 1) slightly prolonged graft survival time from 7 to 11 days. The combination of cyclosporine with donor Buf 3M KCl antigen or with a Buf blood transfurion administered one day prior to transplantation caused greater prolongation of graft survival--19 and 25 days, respectively. Neither third-party BN soluble antigen nor BN blood transfustions acted synergistically with cyclosporine to prolong Buf graft survival. Increasing doses of donor-soluble antigen up to an optimal dose of 5 mg proportionately prolonged graft survival; however, administration of 10 mg antigen was less effective than 5 mg. On the other hand, administration of 1 ml of donor blood achieved the maximal effect. Lymphocytes harvested ten days after transplantation from recipients that had received combined therapy with cyclosporine and donor 3M KCl antigen not only displayed specific unresponsiveness to donor stimulator cells in mixed lymphocyte culture, but also specifically suppressed the proliferative response of syngeneic, virgin WFu responder cells to allogeneic donor Buf but not to third-party BN cells. Furthermore, suppressor cell activity was suggested by diminished responses in an in vivo local adoptive mixed lymphocyte culture assay and by prolongation of Buf kidney survival following systemic adoptive transfer. These findings suggest that immunosuppression with cyclosporine permits induction of specific suppressor cells by 3M KCl donor antigen, resulting in specific unresponsiveness to allografts.
Sialic acids as important molecules in the regulation of the immune system: pathophysiological implications of sialidases in immunity (1993)

Journal Article Sialic acids as important molecules in the regulation of the immune system: pathophysiological implications of sialidases in immunity Get access Yannick Pilatte, Yannick Pilatte 1 Groupe d'Immunoparthologie Pulmonaire, INSERM U 139, Hopital H.Mondor94010 Cretéil, France 1To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Jean Bignon, Jean Bignon Groupe d'Immunoparthologie Pulmonaire, INSERM U 139, Hopital H.Mondor94010 Cretéil, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Claude R. Lambré Claude R. Lambré Groupe d'Immunoparthologie Pulmonaire, INSERM U 139, Hopital H.Mondor94010 Cretéil, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Glycobiology, Volume 3, Issue 3, June 1993, Pages 201–218, https://doi.org/10.1093/glycob/3.3.201 Published: 01 June 1993 Article history Received: 03 March 1993 Accepted: 13 March 1993 Published: 01 June 1993
Organophosphorus compounds. XVIII. Synthesis of 2-Phenyl-2,3-dihydro-1H-1,2-benzazaphosphole 2-sulfide by pyrolysis of (2-Aminobenzyl)phenyldithiophosphinic acid (1983)

Reaction of 2-phthalimidobenzyl bromide (12b) with the dialkyl phenylphosphonites (13a-c) afforded the corresponding alkyl phosphinates (14a-c) which upon hydrazinolysis yielded the respective (2-aminobenzy1)phenylphosphinates (16a-c). Reduction of the phosphinates (16a) or (16b) with lithium aluminium hydride gave (2-aminobenzyl)phenylphosphine (15), characterized by its conversion into crystalline 2,3-diphenyl-1,2,3,4-tetrahydro-1,3-benzazaphosphori(n1e8 ). (2-Aminobenzyl) phenylphosphine (15) was heated with sulfur in benzene under reflux for 30 min to give 80% of (2-aminobenzyl)phenyldithiophosphinic acid (20) which when heated between 100-200° in vacuum underwent elimination of hydrogen sulfide to yield 2-phenyl-2,3-dihydro-1H-l,2-benzazaphosphole 2-sulfide (22a). Several other new phosphorus compounds are described. An attempt to prepare a 1H-1,2-benzazaphosphole derivative by photolysis of methyl benzylphosphonic azide (7) was unsuccessful.
A tele-micro-surgery system across the Internet with a fixed viewpoint/operation-point (2002)

This paper describes the necessary functions and the construction of a micro-surgery system which can be operated from a separate place. In particular, a vision system which has a fixed visual point and a slave manipulator, which has the same fixed point at the end of the tool, are proposed. In the vision system, the axes of all rotational degrees of freedom intersect at the focal point of the microscope. In the slave manipulator, the axes of all rotational degrees of freedom also intersect at this point. These mechanisms provide high accessibility to small objects independent of the approach angle from the microscope and the manipulator to the object. Another feature of the system is the transformation of multi-axis force to auditory information to enhance the operability of the system by indicating the contact state of the end-effector and object. Furthermore, the visual information acquisition and display systems are controlled in accordance with the inferred intention of the operator. Finally, a tele-micro-handling experiment was performed using the Internet and two artificial satellites.
Time-multiplexed light field display with 120-degree wide viewing angle (2019)

The light field display can provide vivid and natural 3D performance, which can find many applications, such as relics research and exhibition. However, current light field displays are constrained by the viewing angle, which cannot meet the expectations. With three groups directional backlights and a fast-switching LCD panel, a time-multiplexed light field display with a 120-degree wide viewing angle is demonstrated. Up to 192 views are constructed within the viewing range to ensure the right geometric occlusion and smooth parallax motion. Clear 3D images can be perceived at the entire range of viewing angle. Additionally, the designed holographic functional screen is used to recompose the light distribution and the compound aspheric lens array is optimized to balance the aberrations and improve the 3D display quality. Experimental results verify that the proposed light field display has the capability to present realistic 3D images of historical relics in 120-degree wide viewing angle.
A Simple Method for Introducing -SH/COOH Group at 5′-OH end of Oligonucleotide (1992)

Abstract A method for introducing a -SH group at the 5′-end of the oligonucleotide using S-Trityl-L-Cysteine has been developed. Carbonyl diimidazole was used for activating the 5′-OH group.
Conversion of aminoglycosidic antibiotics: novel and efficient approaches to 3'-deoxyaminoglycosides via 3'-phosphoryl esters (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConversion of aminoglycosidic antibiotics: novel and efficient approaches to 3'-deoxyaminoglycosides via 3'-phosphoryl estersTetsuya Okutani, Tsunehiko Asako, Kouichi Yoshioka, Kentaro Hiraga, and Makoto KidaCite this: J. Am. Chem. Soc. 1977, 99, 4, 1278–1279Publication Date (Print):February 1, 1977Publication History Published online1 May 2002Published inissue 1 February 1977https://pubs.acs.org/doi/10.1021/ja00446a058https://doi.org/10.1021/ja00446a058research-articleACS PublicationsRequest reuse permissionsArticle Views117Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Molecular Recognition of Agonist Ligands by RXRs (2002)

The nuclear receptor RXR is an obligate partner in many signal transduction pathways. We report the high-resolution structures of two complexes of the human RXRalpha ligand-binding domain specifically bound to two different and chemically unrelated agonist compounds: docosa hexaenoic acid, a natural derivative of eicosanoic acid, present in mammalian cells and recently identified as a potential endogenous RXR ligand in the mouse brain, and the synthetic ligand BMS 649. In both structures the RXR-ligand-binding domain forms homodimers and exhibits the active conformation previously observed with 9-cis-RA. Analysis of the differences in ligand-protein contacts (predominantly van der Waals forces) and binding cavity geometries and volumes for the several agonist-bound RXR structures clarifies the structural features important for ligand recognition. The L-shaped ligand-binding pocket adapts to the diverse ligands, especially at the level of residue N306, which might thus constitute a new target for drug-design. Despite its highest affinity 9-cis-RA displays the lowest number of ligand-protein contacts. These structural results support the idea that docosa hexaenoic acid and related fatty acids could be natural agonists of RXRs and question the real nature of the endogenous ligand(s) in mammalian cells.
Positive contribution of ERdj5/JPDI to endoplasmic reticulum protein quality control in the salivary gland (2009)

In eukaryotic cells, most membrane and secretory proteins are modified post-translationally in the ER (endoplasmic reticulum) for correct folding and assembly. Disulfide-bond formation is one of the important modifications affecting folding and is catalysed by the PDI (protein disulfide isomerase) family proteins. ERdj5 [also known as JPDI (J-domain-containing PDI-like protein)] is a member of the PDI family proteins and has been reported to act as a reductase in ERAD (ER-associated degradation). However, the role of ERdj5 at the whole-body level remains unclear. Therefore in the present study we generated ERdj5-knockout mice {the mouse gene of ERdj5 is known as Dnajc10 [DnaJ (Hsp40) homologue, subfamily C, member 10]} and analysed them. Although ERdj5-knockout mice were viable and healthy, the ER stress response was activated in the salivary gland of the knockout mice more than that of control mice. Furthermore, in ERdj5-knockout cells, the expression of exogenous ERdj5 mitigated the ER stress caused by overproduction of alpha-amylase, which is one of the most abundant proteins in saliva and has five intramolecular disulfide bonds. This effect was dependent on the thioredoxin-like motifs of ERdj5. Thus we suggest that ERdj5 contributes to ER protein quality control in the salivary gland.
Anaesthesia for bullectomy (1985)

A patient with severe bullous emphysema in whom one bulla occupied more than 25% of the hemithorax, with a shift of the mediastinum to the opposite side, was anaesthetised for bullectomy with a combination of intravenous gamma-hydroxybutyric acid and a high thoracic extradural catheter technique. During the operation the patient breathed 100% oxygen through a double-lumen endobronchial tube in the lateral thoracotomy position. Extradural analgesia during the operation was provided by bupivacaine 0.25%, with adrenaline 1:200 000 and, postoperatively, with nicomorphine diluted in dextrose 5%. The advantages of this technique are described.
Dose Effects of Recombinant Adenovirus Immunization in Rodents (2019)

Recombinant adenovirus type 5 (rAd) has been used as a vaccine platform against many infectious diseases and has been shown to be an effective vaccine vector. The dose of the vaccine varies significantly from study to study, making it very difficult to compare immune responses and vaccine efficacy. This study determined the immune correlates induced by serial dilutions of rAd vaccines delivered intramuscularly (IM) and intranasally (IN) to mice and rats. When immunized IM, mice had substantially higher antibody responses at the higher vaccine doses, whereas, the IN immunized mice showed a lower response to the higher rAd vaccine doses. Rats did not show dose-dependent antibody responses to increasing vaccine doses. The IM immunized mice and rats also showed significant dose-dependent T cell responses to the rAd vaccine. However, the T cell immunity plateaued in both mice and rats at 10<sup>9</sup> and 10<sup>10</sup> vp/animal, respectively. Additionally, the highest dose of vaccine in mice and rats did not improve the T cell responses. A final vaccine analysis using a lethal influenza virus challenge showed that despite the differences in the immune responses observed in the mice, the mice had very similar patterns of protection. This indicates that rAd vaccines induced dose-dependent immune responses, especially in IM immunized animals, and that immune correlates are not as predictive of protection as initially thought.
Fast decoder side motion vector derivation for inter frame video coding (2009)

Decoder-side motion vector derivation (DMVD) using template matching has been shown to improve coding efficiency of H.264/AVC based video coding. Instead of explicitly coding motion vectors into the bitstream, the decoder performs motion estimation in order to derive the motion vector used for motion compensated prediction. In previous works, DMVD was performed using a full template matching search in a limited search range. In this paper, a candidate based fast search algorithm replaces the full search. While the complexity reduction especially for the decoder is quite significant, the coding efficiency remains comparable. While for the full search algorithm BD-bitrate savings of 7.4% averaged over CIF and HD sequences according to the VCEG common conditions for IPPP high profile are observed, the proposed fast search achieves bitrate reductions of up to 7.5% on average. By further omitting sub-pel refinement, average savings observed for CIF and HD are still up to 7%.
Assessment of platelet function in the laboratory (2009)

Platelet function testing is essential for the diagnosis of congenital/acquired bleeding disorders and may be useful for the prediction of surgical bleeding. Nowadays there is also much interest in monitoring the efficacy of anti-platelet therapy and measuring platelet hyper-function. However, this often presents clinical laboratories with significant challenges as platelet function tests are complex, poorly standardized, time consuming and quality assurance is not straightforward. There are also few comprehensive modern guidelines available and many recent published surveys have revealed poor standardization between laboratories. Up until the late 1980's the traditional clinical platelet function tests that were available were the bleeding time (BT), light transmission (LTA) and whole blood aggregometry (WBA) and various biochemical assays. These were also usually performed within specialized research and clinical laboratories. Since the last BCSH guidelines were published in 1988 a variety of new platelet function tests have become available. These include flow cytometry and an ever increasing choice of new commercial instruments. Although the potential clinical utility of the new assays is emerging some have not yet entered into routine clinical practice. It is encouraging that a number of standardization committees (e. g. CLSI, BCSH and ISTH Platelet Physiology SSC) are now beginning to produce new platelet function testing guidelines and this will hopefully improve clinical practice, quality assurance and result in less variability between different laboratories.
The polypeptide composition of the mitochondrial NADH: ubiquinone reductase complex from several mammalian species (1985)

The polypeptide composition of isolated mitochondrial NADH:ubiquinone reductase (NADH dehydrogenase) is very similar to that of material immunoprecipitated from detergent-solubilized bovine heart submitochondrial particles by antisera to the holoenzyme. The specificity of the antisera for dehydrogenase polypeptides was determined by immunoblotting, which showed that antisera reacting with only a few proteins were able to immunoprecipitate all others in parallel. The polypeptide compositions of rat, rabbit and human NADH dehydrogenase were determined by immunoprecipitation of the enzyme from solubilized submitochondrial particles and proved to be very similar to that of the bovine heart enzyme, particularly in the high-Mr region. Further homologies in these and other species were explored by immunoblotting with antisera to the holoenzyme and monospecific antisera raised against iron-sulphur-protein subunits of the enzyme.