gebhart/patrepeval huggingface dataset:
Identification of novel regulators in T-cell differentiation of aplastic anemia patients (2006)

Our study suggests a pivotal role of Th1/Tc1 differentiated T-cells in immune-mediated marrow destruction of AA patients. Most importantly, immune regulatory genes could be identified, which are likely involved in the recovery of hematopoiesis and may help to design new therapeutic strategies in bone marrow failure syndromes.
Tandem mass spectrometry in the study of fatty acids, bile acids, and steroids (2003)

Over the last 50 years, the mass spectrometry of lipids has evolved to become one of the most mature techniques in biomolecule analysis. Many volatile and non-polar lipids are directly amenable to analysis by gas-chromatography-mass spectrometry (GC-MS), a technique that combines the unsurpassed separation properties of gas-chromatography with the sensitivity and selectivity of electron ionization mass spectrometry. Less volatile and/or thermally labile lipids can be analyzed by GC-MS, following appropriate sample derivatization. However, many complex lipids are not readily analyzed by GC-MS, and it is these molecules that are the subject of the current review. Since the early 1970s, there have been three outstanding developments in mass spectrometry that are particularly appropriate in lipid analysis; i.e., the introduction of (i) fast atom bombardment (FAB); (ii) electrospray (ES); and (iii) tandem mass spectrometry (MS/MS). The FAB and ES ionization techniques will be discussed in relation to MS/MS, and examples of their application in biochemical studies will be presented. The review will concentrate on the analysis of fatty acids, bile acids, steroid conjugates, and neutral steroids.
Bromodomain coactivators in cancer, obesity, type 2 diabetes, and inflammation. (2010)

Double bromodomain proteins bind to acetylated lysines in histones, bringing associated histone modification and nucleosome remodeling activity to chromatin. The ability of bromodomain regulators to alter chromatin status and control gene expression has long been appreciated to be important in the development of certain human cancers. However, bromodomain proteins have now been found also to be critical, non-redundant players in diverse, non-malignant phenotypes, directing transcriptional programs that control adipogenesis, energy metabolism and inflammation. The fact that such different processes are functionally linked by the same molecular machinery suggests a common epigenetic basis to understand and interpret the origins of several important co-morbidities, such as asthma or cancer that occurs in obesity, and complex inflammatory diseases like cardiovascular disease, systemic lupus erythematosus, rheumatoid arthritis and insulin resistance that may be built on a common pro-inflammatory foundation.
Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics (2017)

There is growing interest in using embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) derivatives for tissue regeneration. However, an increased understanding of human immune responses to stem cell-derived allografts is necessary for maintaining long-term graft persistence. To model this alloimmunity, humanized mice engrafted with human hematopoietic and immune cells could prove to be useful. In this study, an in-depth analysis of graft-infiltrating human lymphocytes and splenocytes revealed that humanized mice incompletely model human immune responses toward allogeneic stem cells and their derivatives. Furthermore, using an "allogenized" mouse model, we show the feasibility of reconstituting immunodeficient mice with a functional mouse immune system and describe a key role of innate immune cells in the rejection of mouse stem cell allografts.
Impact of Lowering the Screening and Confirmation Cutoff Values for Urine Drug Testing Based on Dilution Indicators (2003)

Many clinical and forensic toxicology laboratories establish criteria for identifying a random urine specimen submitted for drug screening as being "normally concentrated" or "dilute" by incorporating creatinine analysis and/or specific gravity measurement into their testing protocols. The objective of this study is to describe the importance of urine creatinine analysis and specific gravity measurements in the Correctional Service of Canada (CSC) drug-testing program. The CSC program uses the Substance Abuse and Mental Health Services Administration (SAMHSA) creatinine cutoff value (20 mg/dL) mandated for workplace drug testing in the United States. In the CSC program, urine specimens must have a creatinine concentration <20 mg/dL and specific gravity value </=1.003 to be considered dilute. The CSC program also incorporates lower drug/drug metabolite screening and confirmation cutoff values (dilution protocol) for specimens that are administratively defined as dilute. Seventy-nine hundred and twelve urine specimens from 2000 to 2002 (6.8% of total workload) were defined as dilute. Twenty-six percent of all dilute specimens (n = 2054) screened positive for one or more drugs using the SAMHSA cutoff values. The screening-negative dilute specimens were taken through the dilution protocol scheme with lower screening cutoff values and confirmation cutoff concentrations at the lower limits of quantification (LLOQ) for each method. Over 1100 of the 5858 dilute urine specimens (18.8%) confirmed positive for one or more drugs in 2000 to 2002 when taken through the dilution protocol scheme. CSC workload is separated based on whether specimens are referred from institutions or from community settings (such as parolee programs). The positive rate for dilute specimens averaged 18.2% from CSC institutions and 22.3% from specimens collected from parolee specimens in 2000 to 2002. The drugs most often confirmed in dilute specimens from institutions were cannabinoids (annual positive rate ranged from 13.7 to 18%) and codeine and/or morphine (ranged from 0.2 to 2.8%). The drugs most often confirmed in dilute urine specimens from community settings in 2000-2002 were cannabinoids (annual positive rate ranged from 10.3 to 12.5%) and cocaine metabolite (ranged from 6.6 to 10.3%). In conclusion, one can reduce the false-negative rate for drugs of abuse in urine drug testing programs by incorporating lower screening and confirmation cutoff (eg, LLOQ) concentrations for dilute specimens that screen negative for drugs of abuse when using the SAMHSA mandated screening and confirmation cutoff concentrations.
WNT5A is a Key Regulator of the Epithelial-Mesenchymal Transition and Cancer Stem Cell Properties in Human Gastric Carcinoma Cells (2013)

WNT5A regulates the induction of EMT and the maintenance of CSC properties in MKN-7 cells. WNT5A may play an important role in constructing an advantageous tumor microenvironment for the progression and development of human GC.
Characterization of fibroblast growth factor receptor 2 overexpression in the human breast cancer cell line SUM-52PE (2000)

The fibroblast growth factor receptor (FGFR)2 gene has been shown to be amplified in 5-10% of breast cancer patients. A breast cancer cell line developed in our laboratory, SUM-52PE, was shown to have a 12-fold amplification of the FGFR2 gene, and FGFR2 message was found to be overexpressed 40-fold in SUM-52PE cells as compared with normal human mammary epithelial (HME) cells. Both human breast cancer (HBC) cell lines and HME cells expressed two FGFR2 isoforms, whereas SUM-52PE cells overexpressed those two isoforms, as well as several unique FGFR2 polypeptides. SUM-52PE cells expressed exclusively FGFR2-IIIb isoforms, which are high-affinity receptors for fibroblast growth factor (FGF)-1 and FGF-7. Differences were identified in the expression of the extracellular Ig-like domains, acid box and carboxyl termini, and several variants not previously reported were isolated from these cells.
Why does output normalization create problems in multiple classifier systems? (2003)

A combination of classifiers is a promising direction for obtaining better classification systems. However the outputs of different classifiers may have different scales and hence the classifier outputs are incomparable. Incomparability of the classifier output scores is a major problem in the combination of different classification systems. In order to avoid this problem, the measurement level classifier outputs are generally normalized. However recent studies have proven that output normalization may provide some problems. For instance, the multiple classifier system's performance may become worse than that of a single individual classifier. This paper presents some interesting observations about the reason why such undesirable behavior occurs.
Flexible Multi-Bit Feedback Design for HARQ Operation of Large-Size Data Packets in 5G (2017)

A reliable feedback channel is vital to report decoding acknowledgments in retransmission mechanisms such as the hybrid automatic repeat request (HARQ). While the feedback bits are known to be costly for the wireless link, a feedback message more informative than the conventional single-bit feedback can increase resource utilization efficiency. Considering the practical limitations for increasing feedback message size, this paper proposes a framework for the design of flexible-content multi-bit feedback. The proposed design is capable of efficiently indicating the faulty segments of a failed large-size data packet thanks to which the transmitter node can reduce the retransmission size to only include the initially failed segments of the packet. We study the effect of feedback size on retransmission efficiency through extensive link-level simulations over realistic channel models. Numerical result present significant savings in retransmission resources offered by the proposed flexible-content feedback design.
On 2-Generator Subgroups of SO(3) (1999)

We classify all subgroups of $SO(3)$ that are generated by two elements, each a rotation of finite order, about axes separated by an angle that is a rational multiple of $\pi$. In all cases we give a presentation of the subgroup. In most cases the subgroup is the free product, or the amalgamated free product, of cyclic groups or dihedral groups. The relations between the generators are all simple consequences of standard facts about rotations by $\pi$ and $\pi /2$. Embedded in the subgroups are explicit free groups on 2 generators, as used in the Banach-Tarski paradox.
Platelet inhibitory activity and pharmacokinetics of prasugrel (CS-747) a novel thienopyridine P2Y12 inhibitor: A multiple-dose study in healthy humans (2006)

AbstractThis double-blind, placebo-controlled trial evaluated the safety, pharmacodynamics, and pharmacokinetics of prasugrel (CS-747, LY640315), a novel thienopyridine P2Y12 ADP receptor antagonist, during multiple oral dosing in healthy subjects. Eighteen subjects received placebo, or prasugrel 2.5 or 10 mg, orally, daily for 10 days. Adverse events were recorded and blood samples for measurement of platelet aggregation, bleeding time, and prasugrel metabolite concentrations were obtained. Two bleeding events were experienced in the prasugrel 10 mg dose group and one in the placebo group. Neither of the events was considered serious. ADP-induced platelet aggregation accumulated over the 10-day study period, reaching a steady state by days 2–4 following administration of prasugrel 10 mg daily. Limited inhibition of platelet aggregation was obtained with prasugrel 2.5 mg. In the 10-mg dose group at day 5, 4 h postdose, with 20 µM ADP as the agonist, inhibition of platelet aggregation was 61.2 ± 5.6 vs. 17.9 ± 6.2% in the placebo group (P < 0.01). Recovery of platelet aggregation was observed 48 h after the last dose of prasugrel 10 mg, but was only partial, consistent with irreversible platelet inhibition. The mean bleeding time was prolonged by day 5 at 4 h postdose in all subjects in the prasugrel 10 mg dose group (prasugrel 10 mg, 1058 ± 412 s vs. placebo, 196 ± 74 s; P < 0.001). The maximum plasma concentration of metabolites increased with prasugrel dose and there was no accumulation of metabolites over the 10-day study period. This study indicated that prasugrel 10 mg daily for 10 days was well tolerated and at steady-state provided sustained high levels of inhibition of platelet aggregation.KeywordsPrasugrelCS-747plateletsphase 1thienopyridine
Pyridazines. VI. Some 6‐substituted 3(2H)pyridazinones (1974)

Abstract A series of 6‐substituted 3(2 H )pyridazinones was prepared. The 6‐substituent was either aromatic of heter‐aromatic in structure. A few basically‐substituted representatives were also made.
Management of atrial fibrillation in the elderly. (2009)

Atrial fibrillation (AF) is associated with a higher incidence of mortality, stroke, and coronary events than is sinus rhythm. AF with a rapid ventricular rate may cause a tachycardia-related cardiomyopathy. Immediate direct-current (DC) cardioversion should be performed in patients with AF and acute myocardial infarction, chest pain due to myocardial ischemia, hypotension, severe heart failure, or syncope. Intravenous beta blockers, diltiazem, or verapamil may be administered to slow immediately a very rapid ventricular rate in AF. An oral beta blocker, verapamil, or diltiazem should be used in persons with AF if a fast ventricular rate occurs at rest or during exercise despite digoxin. Amiodarone may be used in selected patients with symptomatic life-threatening AF refractory to other drugs. Digoxin should not be used to treat patients with paroxysmal AF. Nondrug therapies should be performed in patients with symptomatic AF in whom a rapid ventricular rate cannot be slowed by drugs. Paroxysmal AF associated with the tachycardia-bradycardia syndrome should be treated with a permanent pacemaker in combination with drugs. A permanent pacemaker should be implanted in patients with AF and symptoms such as dizziness or syncope associated with ventricular pauses greater than 3 seconds which are not drug-induced. Elective DC cardioversion has a higher success rate and a lower incidence of cardiac adverse effects than does medical cardioversion in converting AF to sinus rhythm. Unless transesophageal echocardiography has shown no thrombus in the left atrial appendage before cardioversion, oral warfarin should be given for 3 weeks before elective DC or drug cardioversion of AF and continued for at least 4 weeks after maintenance of sinus rhythm. Many cardiologists prefer, especially in older patients, ventricular rate control plus warfarin rather than maintaining sinus rhythm with antiar-rhythmic drugs. Patients with chronic or paroxysmal AF at high risk for stroke should be treated with long-term warfarin to achieve an International Normalized Ratio of 2.0 to 3.0. Patients with AF at low risk for stroke or with contraindications to warfarin should be treated with aspirin 325 mg daily.
Endoscopic Treatment of Vesicoureteral Reflux with a Chondrocyte-Alginate Suspension (1994)

Injection of polytetrafluoroethylene (Teflon) or collagen has been used in the endoscopic treatment of vesicoureteral reflux. Although the principle of an endoscopic treatment is valid, there are concerns regarding the long-term safety and effectiveness of these substances. In search of a different injectable material we conducted experiments using chondrocytes in a biodegradable polymer solution for the treatment of vesicoureteral reflux in an animal model. Reflux was created in 4 mini-pigs and confirmed with a cystogram. Cartilage was obtained from the auricular surface of each animal. Chondrocytes were harvested and expanded in vitro. The cells were individually quantitated and concentrated to 40 million cells per cc. The cell suspensions were mixed with a sodium alginate and calcium sulfate solution. Each pig was injected unilaterally in the subureteral region with the autologous chondrocyte suspension. The opposite ureter served as an internal control in all animals. Cystograms showed resolution of reflux in the treated side and persistence of reflux in the opposite untreated side in each instance. Excretory urograms revealed no evidence of obstruction. Histological examination of the subureteral region demonstrated cartilage. Autologous chondrocytes can be readily harvested, expanded in vitro and injected cystoscopically. The cells survive and form a cartilage nidus that is nonantigenic. This system is able to correct reflux without any evidence of obstruction.
Multi-finger chords for hand-held tablets (2014)

Despite the demonstrated benefits of multi-finger input, todays gesture vocabularies offer a limited number of postures and gestures. Previous research designed several posture sets, but does not address the limited human capacity of retaining them. We present a multi-finger chord vocabulary, introduce a novel hand-centric approach to detect the identity of fingers on off-the-shelf hand-held tablets, and report on the detection accuracy. A between-subjects experiment comparing "random" to a "categorized" chord-command mapping found that users retained categorized mappings more accurately over one week than random ones. In response to the logical posture-language structure, people adapted to logical memorization strategies, such as 'exclusion', 'order', and 'category', to minimize the amount of information to retain. We conclude that structured chord-command mappings support learning, short-, and long-term retention of chord- command mappings.
Administration of Agonistic Anti-4-1BB Monoclonal Antibody Leads to the Amelioration of Experimental Autoimmune Encephalomyelitis (2002)

4-1BB, a member of the TNFR superfamily, is a costimulatory receptor primarily expressed on activated T cells. It has been shown that the administration of agonistic anti-4-1BB Abs enhances tumor immunity and allogenic immune responses. Paradoxically, we found that the administration of an agonistic anti-4-1BB mAb (2A) dramatically reduced the incidence and severity of experimental autoimmune encephalomyelitis (EAE). Adoptive transfer of T cells from such treated mice failed to induce EAE, whereas anti-4-1BB treatment following adoptive transfer of encephalitogenic T cells did not prevent EAE pathogenesis. These results suggest that anti-4-1BB treatment during the induction phase inhibits autoreactive T cell immune responses rather than preventing T cell trafficking into the CNS. This was substantiated by the observations that draining lymph node cells from anti-4-1BB-treated mice failed to respond to Ag stimulation in vitro. In addition, we found that such treatment initially promotes the activation and proliferation of Ag-specific CD4+ T cells but subsequently increases their probability of undergoing activation-induced cell death, thereby inhibiting effector T cell responses. More importantly, 2A treatment also inhibits the relapse of EAE in a clinically relevant murine model of multiple sclerosis. This study indicates that the agonistic Ab against 4-1BB can potentially be used as a novel immunotherapeutic agent for treating autoimmune diseases.
Motor response of the human isolated small intestine and urinary bladder to porcine neuromedin U‐8 (1990)

1. Porcine neuromedin U-8 produced a concentration (0.3 nM-1 microM)-dependent contraction of the longitudinal muscle of the human isolated ileum, which was unaffected by either atropine (1 microM) or tetrodotoxin (1 microM). 2. By contrast, neuromedin U-8 had only a weak effect on the circular muscle of the human isolated ileum. 3. Neuromedin U-8 also produced a concentration-dependent contraction of mucosa-free muscle strips from the dome of the human isolated urinary bladder, its action being unaffected by either atropine or tetrodotoxin. 4. These findings indicate that neuromedin U-8 exerts a direct contractile effect on smooth muscle of the human intestinal and urinary tract.
Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease. (1979)

The recognition sequence for the dam methylase of Escherichia coli K12 has been determined directly by use of in vivo methylated ColE1 DNA or DNA methylated in vitro with purified enzyme. The methylase recognizes the symmetric tetranucleotide d(pG-A-T-C) and introduces two methyl groups per site in duplex DNA with the product of methylation being 6-methylaminopurine. This work has also demonstrated that Dpn I restriction endonuclease cleaves on the 3' side of the modified adenine within the methylated sequence to yield DNA fragments possessing fully base-paired termini. All sequences in ColE1 DNA methylated by the dam enzyme are subject to double strand cleavage by Dpn I endonuclease. Therefore, this restriction enzyme can be employed for mapping the location of sequences possessing the dam modification.
Essential Oil Composition ofGmelina arboreaRoxb., Verbenaceae, From Nigeria (2009)

Abstract The chemical composition of the fruit oil of Gmelina arborea Roxb., Verbenaceae, was examined by capillary gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). One hundred components were identified which accounted for 92.7% of the oil including 24 trace compounds. The ubiquitous terpenes occurred in lesser amount. The fruit oil was characterized by the abundance of (Z)-3-hexenol (17.9%), 1-octen-3-ol (8.4%) and hexanol (6.1%) among the aliphatic alcohols; heptacosane (5.6%), pentacosane (3.8%) and 1-pentacosene (3.2%) among the hydrocarbons; and nonanal (8.7%) and (E)-2-decenal (3.0%) as the main aldehyde constituents.
In vitro activity of fosfomycin combined with rifampin, pefloxacin and imipenem against staphylococci: a study by the time-kill curve method. (1987)

The in vitro activity of fosfomycin alone and in combination with rifampin, pefloxacin and imipenem was studied by the time-kill method against staphylococci. Fosfomycin, pefloxacin and imipenem used at concentrations within the therapeutic range, exerted a bactericidal effect, whereas rifampin acted as a bacteriostatic drug. The combination of fosfomycin and rifampin was found to be antagonistic against rifampin-susceptible strains and indifferent for rifampin-resistant isolates. Fosfomycin combined with pefloxacin usually produced an indifferent effect. The interaction between fosfomycin and imipenem was mainly indifferent but synergism occurred with methicillin-resistant strains and antagonism was observed for one methicillin-susceptible isolate of Staphylococcus aureus.
SF-36 Health Survey Update (2000)

From QualityMetric, Inc.; Lincoln, Rhode Island; and the Health Assessment Lab; Tufts Medical School, and Harvard School of Public Health, Boston, Massachusetts. Address reprint requests to John E. Ware, Jr., PhD QualityMetric, Inc. 640 George Washington Highway Lincoln, RI 02865 E-mail: [email protected] Development and validation of the SF-36 Health Survey was supported by a grant from the Henry J. Kaiser Family Foundation to The Health Institute, New England Medical Center (J. E. Ware, Jr., Principal Investigator). Development of the SF-36 PCS and MCS summary measures was supported by unrestricted research grants for the International Quality of Life Assessment (IQOLA) Project for the Glaxo Research Institute, Research Triangle Park, NC and Schering-Plough Corp., Kenilworth, NJ (J. E. Ware, Jr., Principal Investigator). The IQOLA Project is sponsored by unrestricted research grants from GlaxoWellcome Inc., Research Triangle Park, NC, and Schering-Plough Corp., Kenilworth, NJ. Associate sponsors include Astra, Parke-Davis, Pharmacia & Upjohn, Proctor & Gamble Pharmaceuticals, Searle, Solvay Duphar B.V., and Synthelabo. Additional support has also been provided by more than 40 other pharmaceutical companies.