gebhart/patrepeval huggingface dataset:
Multiple-Camera Tracking of Rigid Objects (2002)

In this paper we describe a method for tracking rigid objects using one or several cameras. The tracking process consists of aligning a 3-D model representation of an object with image contours by measuring and minimizing the image error between predicted model points and image contours. The tracker behaves like a visual servo loop where the internal and external camera parameters are updated at each new image acquisition. We study in detail the Jacobian matrix associated with this minimization process in the presence of both point-to-point and point-to-contour matches. We establish the minimal number of matches that are needed as well as the singular configurations leading to a rank-deficient Jacobian matrix. We find a mathematical link between the point-to-point and point-to-contour cases. Based on this link we show that the latter has the same kind of singularities as the former. Moreover, we study multiple-camera configurations which optimize the robustness of the method in the presence of single-camera singularities, bad, noisy, or missing data. Extensive experiments done with a complex ship part and with up to three cameras validate the method. In particular we show that the tracker may well be used as a camera calibration procedure.
A Survey on Dynamic Energy Management at Virtualization Level in Cloud Data Centers (2013)

in increased energy consumption. This necessitates the development of efficient energy management techniques in data center not only for operational cost but also to reduce the amount of heat released from storage devices. Virtualization is a powerful tool for energy management that achieves efficient utilization of data center resources. Though, energy management at data centers can be static or dynamic, virtualization level energy management techniques contributes more energy conservation than hardware level. This paper surveys various issues related to dynamic energy management at virtualization level in cloud data centers.
Renal Fibrosis Is Significantly Attenuated Following Targeted Disruption of Cd40 in Experimental Renal Ischemia (2020)

Background Renal artery stenosis is a common cause of renal ischemia, contributing to the development of chronic kidney disease. To investigate the role of local CD40 expression in renal artery stenosis, Goldblatt 2-kidney 1-clip surgery was performed on hypertensive Dahl salt-sensitive rats (S rats) and genetically modified S rats in which CD40 function is abolished (<i>Cd40</i><sup><i>mutant</i></sup>). Methods and Results Four weeks following the 2-kidney 1-clip procedure, <i>Cd40</i><sup><i>mutant</i></sup> rats demonstrated significantly reduced blood pressure and renal fibrosis in the ischemic kidneys compared with S rat controls. Similarly, disruption of Cd40 resulted in reduced 24-hour urinary protein excretion in <i>Cd40</i><sup><i>mutant</i></sup> rats versus S rat controls (46.2±1.9 versus 118.4±5.3 mg/24 h; <i>P</i><0.01), as well as protection from oxidative stress, as indicated by increased paraoxonase activity in <i>Cd40</i><sup><i>mutant</i></sup> rats versus S rat controls (<i>P</i><0.01). Ischemic kidneys from <i>Cd40</i><sup><i>mutant</i></sup> rats demonstrated a significant decrease in gene expression of the profibrotic mediator, plasminogen activator inhibitor-1 (<i>P</i><0.05), and the proinflammatory mediators, C-C motif chemokine ligand 19 (<i>P</i><0.01), C-X-C Motif Chemokine Ligand 9 (<i>P</i><0.01), and interleukin-6 receptor (<i>P</i><0.001), compared with S rat ischemic kidneys, as assessed by quantitative PCR assay. Reciprocal renal transplantation documented that CD40 exclusively expressed in the kidney contributes to ischemia-induced renal fibrosis. Furthermore, human CD40-knockout proximal tubule epithelial cells suggested that suppression of CD40 signaling significantly inhibited expression of proinflammatory and -fibrotic genes. Conclusions Taken together, our data suggest that activation of CD40 induces a significant proinflammatory and -fibrotic response and represents an attractive therapeutic target for treatment of ischemic renal disease.
MicroRNA deregulation in human thyroid papillary carcinomas (2006)

MicroRNAs (miRNAs) are a class of small non-coding RNAs involved in a wide range of basic processes such as cell proliferation, development, apoptosis and stress response. It has recently been found that they are also abnormally expressed in many types of human cancer. We analyzed the genome-wide miRNA expression profile in human thyroid papillary carcinomas (PTCs) using a microarray (miRNACHIP microarray) containing hundreds of human precursor and mature miRNA oligonucleotide probes. Using this approach, we found an aberrant miRNA expression profile that clearly differentiates PTCs from normal thyroid tissues. In particular, a significant increase in miRNA (miR)-221, -222 and -181b was detected in PTCs in comparison with normal thyroid tissue. These results were further confirmed by northern blot and quantitative RT-PCR analyses. Moreover, RT-PCR revealed miR-221, -222 and -181b overexpression in fine needle aspiration biopsies corresponding to thyroid nodules, which were eventually diagnosed as papillary carcinomas after surgery. Finally, miR-221, -222 and -181b overexpression was also demonstrated in transformed rat thyroid cell lines and in mouse models of thyroid carcinogenesis. Functional studies, performed by blocking miR-221 function and by overexpressing miR-221 in human PTC-derived cell lines, suggest a critical role of miR-221 overexpression in thyroid carcinogenesis. In conclusion, these data, taken together, indicate an miRNA signature associated with PTCs, and suggest miRNA deregulation as an important event in thyroid cell transformation.
Plasma From Preeclamptic Women Increases Blood-Brain Barrier Permeability (2010)

Circulating factors in preeclamptic women are thought to cause endothelial dysfunction and thereby contribute to the progression of this hypertensive condition. Despite the involvement of neurological complications in preeclampsia, there is a paucity of data regarding the effect of circulating factors on cerebrovascular function. Using a rat model of pregnancy, we investigated blood-brain barrier permeability, myogenic activity, and the influence of endothelial vasodilator mechanisms in cerebral vessels exposed intraluminally to plasma from normal pregnant or preeclamptic women. In addition, the role of vascular endothelial growth factor signaling in mediating changes in permeability in response to plasma was investigated. A 3-hour exposure to 20% normal pregnant or preeclamptic plasma increased blood-brain barrier permeability by ≈6.5- and 18.0-fold, respectively, compared with no plasma exposure (P<0.01). Inhibition of vascular endothelial growth factor receptor kinase activity prevented the increase in permeability in response to preeclamptic plasma but had no effect on changes in permeability of vessels exposed to normal pregnant plasma. Circulating factors in preeclamptic plasma did not affect myogenic activity or the influence of endothelium on vascular tone. These findings demonstrate that acute exposure to preeclamptic plasma has little effect on reactivity of cerebral arteries but significantly increases blood-brain barrier permeability. Prevention of increased permeability by inhibition of vascular endothelial growth factor signaling suggests that activation of this pathway may be responsible for increased blood-brain barrier permeability after exposure to preeclamptic plasma.
A Gain of Function TGFB1 Polymorphism May Be Associated With Late Stage Prostate Cancer (2004)

Transforming growth factor beta (TGFbeta) is known to exert both positive and negative effects on different stages of tumor formation. Of the TGFbetaisoforms, TGFbeta1 is highly expressed in prostate cancer and leads to tumor promotion and metastasis. Increased expression of TGFbeta1 is associated with more aggressive tumors and poor prognosis. Several polymorphisms in TGFB1 have been identified, and two variants in strong linkage disequilibrium, C-509T and T+29C, show increased serum levels. Because of the potential role of TGFB1 variants in prostate cancer and progression, we hypothesized that these two TGFB1 variants would be associated with prostate cancer risk, particularly later, more aggressive stage tumors. To test this, we conducted a nested case-control study of 492 men diagnosed with prostate cancer from the Physicians Health Study and 492 age-matched controls. In this study, cases who were homozygous for the T allele at position -509 had a 2.4-fold increased risk of more advanced stage of prostate cancer [95% confidence interval (95% CI) 1.03-5.43; P = 0.04]. The T allele frequencies in cases and controls were 32.7% and 31.4%, respectively. The same polymorphism showed a 1.23 nonsignificant odds ratio (OR) for overall prostate cancer risk (95% CI 0.80-1.87). Cases who were homozygous for the C allele at position +29 did not show any significant increase in risk for either total prostate cancer (OR 1.19, 95% CI 0.82-1.74) or advanced stage prostate cancer (OR 1.33, 95% CI 0.66-2.68). The C allele frequency in cases and controls were 39.9% and 38.5%, respectively. Our data suggest that the TGFB1 C-509T variant that affects expression of TGFbeta1 may play a role in advanced stage prostate cancer.
Secretion of disulfide-linked human T-cell receptor gamma delta heterodimers (1993)

The availability of soluble forms of T-cell antigen receptors (sTCR) should be of great use in the detailed characterization of their interactions with ligands, for the generation of anti-TCR monoclonal antibodies (mAb), and for the eventual determination of their three-dimensional structures by x-ray crystallography. Here, we show that efficient secretion of nonchimeric disulfide-linked human gamma delta TCR could be achieved by simply introducing translational termination codons upstream from the sequences encoding TCR chain transmembrane regions. This recombinant protein appeared to be correctly folded, as judged by its reactivity with a panel of anti-gamma and anti-delta mAbs, and proved to be a powerful immunogen, allowing generation of mAb that are able to recognize both soluble- and membrane-bound gamma delta TCR. While variable and constant domains of gamma delta sTCR seem to be folded into compact conformations, the extreme sensitivity of its interchain disulfide bridge to digestion with papain suggests that sTCR C-terminal portions are in a more extended configuration than the corresponding region in immunoglobulins. Finally, the gamma delta heterodimer remains stable even after removal of the interchain disulfide link, suggesting the existence of strong noncovalent forces holding the two chains together.
A Pilot Study of Therapeutic Vaccination With Envelope Protein E1 in 35 Patients With Chronic Hepatitis C (2003)

New treatments are needed for chronic hepatitis C patients in whom viral clearance cannot be achieved. Thirty-five chronic hepatitis C patients (genotype 1) were randomized to receive 20 mug of recombinant HCV E1 (E1) (n = 26) or placebo (n = 9) intramuscularly at weeks 0, 4, 8, 12, and 24. Thirty-four then received open-label E1 vaccine at weeks 50, 53, 56, 59, 62, and 65. Twenty-four patients (12 men, 12 women; mean age, 52 y; 18 interferon-based treatment failures; mean baseline alanine aminotransferase [ALT] level, 118 IU/L) underwent a biopsy before and after 2 courses of E1, 17 months later. Liver histology was scored by 2 blinded pathologists according to the Ishak and Metavir systems. Postinjection reactions were similar to placebo (alum only). Nine of 24 patients (38%) had improvement of 2 points or more, 10 (41%) remained stable, and 5 (21%) showed worsening in total Ishak score. Nine patients (38%) improved both on Ishak and Metavir fibrosis scores. Plasma HCV-RNA levels remained unchanged, whereas ALT levels showed a trend toward a decrease during treatment. All but 3 patients developed a significant de novo E1-specific T-cell response. The increase in anti-E1 antibody levels correlated with the decrease in total Ishak score and with the relative decreases in both Ishak fibrosis score and ALT level (all P < or =.01). In conclusion, E1 therapeutic vaccination is well tolerated and the observed effects warrant further study.
Synthesis of Poly(styrene) Monolayers Attached to High Surface Area Silica Gels through Self-Assembled Monolayers of Azo Initiators (1998)

We report on the radical-chain polymerization of styrene using self-assembled monolayers of azo initiators covalently bound to high surface area silica gels. In this process monolayers of poly(styrene) molecules terminally attached to the surface of the inorganic substrate are obtained. As the initiator molecules are immobilized at the surfaces in a one-step reaction, well-reproducible layers can be prepared and the surface concentration of the initiator can be adjusted in a wide range between the limit of detection and full surface coverage. In the subsequent polymerization reactions polymer monolayers with high, controlled graft density can be obtained. The synthesis of the attached layers and the characterization by X-ray photoelectron spectroscopy, diffuse reflectance infrared spectroscopy (DRIFT), and elemental analysis are described. After cleavage of an ester group that connects the polymers to the surface, the molecular weights of the polymers were determined. The results of the study show that this “grafting from” technique can be used for the preparation of polymer layers with controlled, high graft densities.
Kinetic discrimination in T-cell activation. (1996)

We propose a quantitative model for T-cell activation in which the rate of dissociation of ligand from T-cell receptors determines the agonist and antagonist properties of the ligand. The ligands are molecular complexes between antigenic peptides and proteins of the major histocompatibility complex on the surfaces of antigen-presenting cells. Binding of ligand to receptor triggers a series of biochemical reactions in the T cell. If the ligand dissociates after these reactions are complete, the T cell receives a positive activation signal. However, dissociation of ligand after completion of the first reaction but prior to generation of the final products results in partial T-cell activation, which acts to suppress a positive response. Such a negative signal is brought about by T-cell ligands containing the variants of antigenic peptides referred to as T-cell receptor antagonists. Results of recent experiments with altered peptide ligands compare favorably with T-cell responses predicted by this model.
Transcription factor GATA-1 potently represses the expression of the HIV-1 coreceptor CCR5 in human T cells and dendritic cells (2005)

CC chemokine receptor 5 (CCR5) is the major HIV-1 coreceptor and its expression levels are a critical determinant of HIV-1 infection. However, the molecular mechanisms of CCR5 regulation in primary targets of HIV-1 remain unknown. Despite binding to conserved DNA elements, we show that the transcription factors GATA binding protein 1 (GATA-1) and GATA-3 differentially suppress the expression of CCR5 in stem-cell-derived dendritic cells and primary human T-cell subsets. In addition, GATA-1 expression was also more potent than GATA-3 in suppressing T helper 1 (Th1)-associated genes, interferon-gamma (IFNgamma), and CXC chemokine receptor-3 (CXCR3). GATA-1, but not GATA-3, potently suppressed CCR5 transcription, thereby rendering human T cells resistant to CCR5-tropic HIV-1 infection. However, GATA-1 could also serve as a surrogate for GATA-3 in its canonic role of programming Th2 gene expression. These findings provide insight into GATA-3-mediated gene regulation during T-cell differentiation. Importantly, decoding the mechanisms of GATA-1-mediated repression of CCR5 may offer an opportunity to develop novel approaches to inhibit CCR5 expression in T cells.
Copper- and copper– N -heterocyclic carbene-catalyzed C─H activating carboxylation of terminal alkynes with CO 2 at ambient conditions (2010)

The use of carbon dioxide as a renewable and environmentally friendly source of carbon in organic synthesis is a highly attractive approach, but its real world applications remain a great challenge. The major obstacles for commercialization of most current protocols are their low catalytic performances, harsh reaction conditions, and limited substrate scope. It is important to develop new reactions and new protocols for CO(2) transformations at mild conditions and in cost-efficient ways. Herein, a copper-catalyzed and copper-N-heterocyclic carbene-cocatalyzed transformation of CO(2) to carboxylic acids via C─H bond activation of terminal alkynes with or without base additives is reported. Various propiolic acids were synthesized in good to excellent yields under ambient conditions without consumption of any organometallic or organic reagent additives. This system has a wide scope of substrates and functional group tolerances and provides a powerful tool for the synthesis of highly functionalized propiolic acids. This catalytic system is a simple and economically viable protocol with great potential in practical applications.
Direct single stranded sequencing from agarose of polymerase chain reaction products (1990)

Journal Article Direct single stranded sequencing from agarose of polymerase chain reaction products Get access A. Green, A. Green 1MRC Molecular Genetics UnitHills Road, Cambridge, CB2 2QH2Department of Clinical Medicine, University of CambridgeLondon, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar A. Roopra, A. Roopra 1MRC Molecular Genetics UnitHills Road, Cambridge, CB2 2QH Search for other works by this author on: Oxford Academic PubMed Google Scholar M. Vaudin M. Vaudin 1MRC Molecular Genetics UnitHills Road, Cambridge, CB2 2QH3ICRF, Lincoln Inn's FieldsLondon, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 20, 25 October 1990, Pages 6163–6164, https://doi.org/10.1093/nar/18.20.6163 Published: 25 October 1990 Article history Received: 13 August 1990 Published: 25 October 1990
Green synthesis of silver and palladium nanoparticles at room temperature using coffee and tea extract (2008)

An extremely simple green approach that generates bulk quantities of nanocrystals of noble metals such as silver (Ag) and palladium (Pd) using coffee and tea extract at room temperature is described. The single-pot method uses no surfactant, capping agent, and/or template. The obtained nanoparticles are in the size range of 20–60 nm and crystallized in face centered cubic symmetry. The method is general and may be extended to other noble metals such as gold (Au) and platinum (Pt).
The Development of Suture Anchors for Use in Soft Tissue Fixation to Bone (1994)

Soft tissue fixation to bone is a basic technique of orthopaedic surgery for which a multitude of devices have been developed. A new class of orthopaedic devices termed "suture anchors" has been developed to secure soft tissues to bone using suture material. The suture anchor is designed to allow quick and secure fixation without the need for extensive exposure. This device was tested using in vitro and in vivo models and found to be comparable with standard transosseous and two-pronged stable techniques. This device should prove useful in allowing the versatility of suture fixation of soft tissues to bone without the technical requirements of constructing transosseous suture tunnels.
Smart sensor system for trace organophosphorus and organosulfur vapor detection employing a temperature-controlled array of surface acoustic wave sensors, automated sample preconcentration, and pattern recognition (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSmart sensor system for trace organophosphorus and organosulfur vapor detection employing a temperature-controlled array of surface acoustic wave sensors, automated sample preconcentration, and pattern recognitionJay W. Grate, Susan L. Rose-Pehrsson, David L. Venezky, Mark. Klusty, and Hank. WohltjenCite this: Anal. Chem. 1993, 65, 14, 1868–1881Publication Date (Print):July 15, 1993Publication History Published online1 May 2002Published inissue 15 July 1993https://pubs.acs.org/doi/10.1021/ac00062a011https://doi.org/10.1021/ac00062a011research-articleACS PublicationsRequest reuse permissionsArticle Views774Altmetric-Citations237LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Incorporating Bortezomib into the Treatment of Lung Cancer (2007)

Bortezomib, a small-molecule proteasome inhibitor, has activity in lung cancer both as a single agent and in combination with agents commonly used in lung cancer. The ability of bortezomib to favorably modulate the expression of apoptosis-associated proteins, along with its moderate toxicity as a single agent, provides the basis for its combination with cytotoxic agents in the treatment of lung cancer. In non-small cell lung cancer, bortezomib as a single agent has limited activity but in combination with chemotherapy has shown encouraging activity without significantly adding to toxicity. Bortezomib as a single agent has shown minimal activity in small cell lung cancer. Although the role of bortezomib in lung cancer is uncertain, it is likely to have its greatest clinical benefit when given in combination with other therapeutics. Ongoing studies are focused on optimizing the scheduling of bortezomib with chemotherapy, investigating its combination with targeted agents and radiation, and examining its efficacy in a specific subgroup, bronchioloalveolar carcinoma.
Rethinking the Warburg Effect with Myc Micromanaging Glutamine Metabolism (2010)

The MYC oncogene, which is frequently deregulated in human cancers, encodes a master transcription factor c-Myc (herein termed Myc) that integrates cell proliferation with metabolism through its regulation of thousands of genes including microRNAs (miRNA). In addition to its known function in regulating the cell cycle and glucose metabolism, recent studies document a role for Myc in stimulating glutamine catabolism, in part through the repression of miRNAs miR-23a and miR-23b. These observations suggest an additional level of complexity in tumor metabolism, which includes the commensal metabolic relationship between hypoxic and nonhypoxic regions of tumors as well as the surrounding stroma. Thus, a reevaluation of cancer metabolism considering glutamine catabolism with a better understanding of the tumor histological complexity is needed before cancer metabolism can be effectively targeted in therapy.