gebhart/patrepeval huggingface dataset:
CORTICO-LIMBIC RESPONSES TO MASKED AFFECTIVE FACES ACROSS PTSD, PANIC DISORDER, AND SPECIFIC PHOBIA (2013)

Background Exaggerated amygdala and reduced ventromedial prefrontal cortex (vmPFC) responsiveness during emotional processing have been reported in studies examining individual anxiety disorders. Studies are needed, however, which directly compare activation of amygdalo-cortical circuitry across multiple anxiety disorders within the same study. Here we compared cortico-limbic neurocircuitry across three different anxiety disorders using a well-validated emotional probe task. Methods Sixty-five adult volunteers, including 22 healthy controls (HC) and participants meeting DSM-IV criteria for either posttraumatic stress disorder (14 PTSD), panic disorder (14 PD), or specific animal phobia (15 SP), underwent functional magnetic resonance imaging (fMRI) at 3 T while passively viewing backward-masked images of faces expressing fear, happy, and neutral emotions. Results A group comprising all three anxiety disorders showed greater activation within the left amygdala and reduced activation within the vmPFC compared to the HC group during the masked fear versus neutral condition. Pairwise group comparisons showed that amygdala activation only reached significance for the PTSD versus HCs, whereas decreased vmPFC was only evident for SP and PD groups versus the HC group. Furthermore, activation did not differ among the anxiety groups when contrasted directly with one another. A similar pattern was observed for masked happy versus neutral faces. Conclusions Exclusive of specific diagnostic category, anxiety disorders were generally associated with increased activation of the amygdala and reduced activation within vmPFC. Categorical distinctions were generally weak or not observed and suggest that functional differences may reflect the magnitude of responses within a common neurocircuitry across disorders rather than activation of distinct systems.
Dynamically scaling applications in the cloud (2011)

Scalability is said to be one of the major advantages brought by the cloud paradigm and, more specifically, the one that makes it different to an "advanced outsourcing" solution. However, there are some important pending issues before making the dreamed automated scaling for applications come true. In this paper, the most notable initiatives towards whole application scalability in cloud environments are presented. We present relevant efforts at the edge of state of the art technology, providing an encompassing overview of the trends they each follow. We also highlight pending challenges that will likely be addressed in new research efforts and present an ideal scalable cloud system.
CFH,VEGFandHTRA1promoter genotype may influence the response to intravitreal ranibizumab therapy for neovascular age-related macular degeneration (2011)

This study reports preliminary evidence suggesting that the higher AMD risk genotypes in CFH, VEGF and HTRA1 may influence the short-term response to treatment with ranibizumab for neovascular AMD.
Therapeutic Use of Stem Cell Transplantation for Cell Replacement or Cytoprotective Effect of Microvesicle Released from Mesenchymal Stem Cell (2014)

Idiopathic pulmonary fibrosis (IPF) is the most common and severe type of idiopathic interstitial pneumonias (IIP), and which is currently no method was developed to restore normal structure and function. There are several reports on therapeutic effects of adult stem cell transplantations in animal models of pulmonary fibrosis. However, little is known about how mesenchymal stem cell (MSC) can repair the IPF. In this study, we try to provide the evidence to show that transplanted mesenchymal stem cells directly replace fibrosis with normal lung cells using IPF model mice. As results, transplanted MSC successfully integrated and differentiated into type II lung cell which express surfactant protein. In the other hand, we examine the therapeutic effects of microvesicle treatment, which were released from mesenchymal stem cells. Though the therapeutic effects of MV treatment is less than that of MSC treatment, MV treat-ment meaningfully reduced the symptom of IPF, such as collagen deposition and inflammation. These data suggest that stem cell transplantation may be an effective strategy for the treatment of pulmonary fibrosis via replacement and cytoprotective effect of microvesicle released from MSCs.
Molecular mechanisms of neurodegeneration in Alzheimer's disease (2010)

Alzheimer's disease (AD) is characterized by cognitive impairment, progressive neurodegeneration and formation of amyloid-beta (Abeta)-containing plaques and neurofibrillary tangles composed of hyperphosphorylated tau. The neurodegenerative process in AD is initially characterized by synaptic damage accompanied by neuronal loss. In addition, recent evidence suggests that alterations in adult neurogenesis in the hippocampus might play a role. Synaptic loss is one of the strongest correlates to the cognitive impairment in patients with AD. Several lines of investigation support the notion that the synaptic pathology and defective neurogenesis in AD are related to progressive accumulation of Abeta oligomers rather than fibrils. Abnormal accumulation of Abeta resulting in the formation of toxic oligomers is the result of an imbalance between the levels of Abeta production, aggregation and clearance. Abeta oligomers might lead to synaptic damage by forming pore-like structures with channel activity; alterations in glutamate receptors; circuitry hyper-excitability; mitochondrial dysfunction; lysosomal failure and alterations in signaling pathways related to synaptic plasticity, neuronal cell and neurogenesis. A number of signaling proteins, including fyn kinase; glycogen synthase kinase-3beta (GSK3beta) and cyclin-dependent kinase-5 (CDK5), are involved in the neurodegenerative progression of AD. Therapies for AD might require the development of anti-aggregation compounds, pro-clearance pathways and blockers of hyperactive signaling pathways.
Cloning and functional analysis of the ubiquitin-specific protease gene UBP1 of Saccharomyces cerevisiae (1991)

In eukaryotes, both natural and engineered fusions of ubiquitin to itself or other proteins are cleaved by processing proteases after the last (Gly76) residue of ubiquitin. Using the method of sib selection, and taking advantage of the fact that bacteria such as Escherichia coli lack ubiquitin-specific enzymes, we have cloned a gene, named UBP1, of the yeast Saccharomyces cerevisiae that encodes a ubiquitin-specific processing protease. With the exception of polyubiquitin, the UBP1 protease cleaves at the carboxyl terminus of the ubiquitin moiety in natural and engineered fusions irrespective of their size or the presence of an amino-terminal ubiquitin extension. These properties of UBP1 distinguish it from the previously cloned yeast protease YUH1, which deubiquitinates relatively short ubiquitin fusions but is virtually inactive with longer fusions such as ubiquitin-beta-galactosidase. The amino acid sequence of the 809-residue UBP1 lacks significant similarities to other known proteins, including the 236-residue YUH1 protease. Null ubp1 mutants are viable, and retain the ability to deubiquitinate ubiquitin-beta-galactosidase, indicating that the family of ubiquitin-specific proteases in yeast is not limited to UBP1 and YUH1.
RNA Polymerase and Transcription Factors (2014)

Gene expression in bacteria is regulated primarily at the level of transcription. Transcription and its regulation have been studied extensively in several gram-negative bacteria, especially Escherichia coli, but mostly neglected in gram-positive bacteria. The major exception is Bacillus subtilis, which because of its amenability to genetic analysis has served as a paradigm for understanding transcription in gram-positive bacteria. This chapter attempts to provide an overview of the roles of the major families of proteins required for transcription and its regulation in B. subtilis. Emphasis is placed on comparing the roles of these factors in B. subtilis and gram-negative bacteria. The nusA product from E. coli, associates with RNA polymerase after initiation of transcription and remains associated with the elongation complex. The sigma subunit of RNA polymerase determines the specificity of promoter utilization. There are at least 10 different sigma factors in B. subtilis, each of which directs RNA polymerase to a different set of promoters. Two regions in most sigma factors in bacteria probably determine the specificity of promoter utilization by making sequence-specific contacts at two regions of promoters located approximately 10 and 35 bp upstream from the start point of transcription. Examples of the use of altered specificity mutations in sigma factors to determine the role of these factors during sporulation are also described.
Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene (2016)

The identification of molecular networks at the system level in mammals is accelerated by next-generation mammalian genetics without crossing, which requires both the efficient production of whole-body biallelic knockout (KO) mice in a single generation and high-performance phenotype analyses. Here, we show that the triple targeting of a single gene using the CRISPR/Cas9 system achieves almost perfect KO efficiency (96%-100%). In addition, we developed a respiration-based fully automated non-invasive sleep phenotyping system, the Snappy Sleep Stager (SSS), for high-performance (95.3% accuracy) sleep/wake staging. Using the triple-target CRISPR and SSS in tandem, we reliably obtained sleep/wake phenotypes, even in double-KO mice. By using this system to comprehensively analyze all of the N-methyl-D-aspartate (NMDA) receptor family members, we found Nr3a as a short-sleeper gene, which is verified by an independent set of triple-target CRISPR. These results demonstrate the application of mammalian reverse genetics without crossing to organism-level systems biology in sleep research.
Enzyme/prodrug gene therapy approach for breast cancer using a recombinant adenovirus expressing Escherichia coli cytosine deaminase. (1997)

A recombinant adenovirus expressing Escherichia coli cytosine deaminase (AdCD) was constructed with the purpose of exploring its utility for the treatment of breast cancer. Infection of the human breast cancer cell line, MDA-MB-231, with AdCD resulted in high levels of cytosine deaminase enzyme activity. MDA-MB-231 cells infected with AdCD were 1000-fold more sensitive to 5-fluorocytosine (5-FC) than cells infected with a control adenovirus. Cell mixing experiments indicated that only 10% of AdCD-infected cells in a population were needed to induce complete cytotoxicity of noninfectious cells exposed to 5-FC. This suggests that bystander effects play an important role in AdCD-mediated cytotoxicities. Direct injection of AdCD into human breast MDA-MB-231-derived tumors grown as xenografts in nude mice, followed by daily intraperitoneal injection 5-FC was sufficient to inhibit tumor growth. These results suggest that in vivo gene therapy for breast cancer using AdCD is feasible.
Présentation générale des Actes du colloque (2001)

Robert André, Etévé Christiane. Présentation générale des Actes du colloque. In: Revue française de pédagogie, volume 135, 2001. Culture et éducation: Colloque en hommage à Jean-Claude Forquin. pp. 9-10.
A minute circular DNA from Escherichia coli 15. (1968)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Design and Implementation of PUF-Based "Unclonable" RFID ICs for Anti-Counterfeiting and Security Applications (2008)

Physical unclonable functions (PUFs) exploit the physical characteristics of the silicon and the IC manufacturing process variations to uniquely characterize each and every silicon chip. Since it is practically impossible to model, copy, or control the IC manufacturing process variations, PUFs not only make these chips unique, but also effectively unclonable. Exploiting the inherent variations in the IC manufacturing process, PUFs provide a secure, robust, low cost mechanism to authenticate silicon chips. This makes PUFs attractive for RFID ICs where cost and security are the key requirements. In this paper we present the design and implementation of PUF enabled "unclonable" RFIDs. The PUF-enabled RFID has been fabricated in 0.18 μ technology, and extensive testing results demonstrate that PUFs can securely authenticate an RFID with minimal overheads. We also highlight the advantages of PUF based RFIDs in anti-counterfeiting and security applications.
A novel method for extracting respiration rate and relative tidal volume from infrared thermography (2011)

In psychophysiological research, measurement of respiration has been dependent on transducers having direct contact with the participant. The current study provides empirical data demonstrating that a noncontact technology, infrared video thermography, can accurately estimate breathing rate and relative tidal volume across a range of breathing patterns. Video tracking algorithms were applied to frame-by-frame thermal images of the face to extract time series of nostril temperature and to generate breath-by-breath measures of respiration rate and relative tidal volume. The thermal indices of respiration were contrasted with criterion measures collected with inductance plethysmography. The strong correlations observed between the technologies demonstrate the potential use of facial video thermography as a noncontact technology to monitor respiration.
Optimal Fraction Collecting in Preparative LC/MS (2004)

In this paper, we discuss various methods for fraction collection in high-throughput chromatography. UV-triggered fractionation allows precise cutting of peaks. However, valuable fraction collector space is wasted, because many undesired compounds are collected. In mass-triggered fraction collection, the collector space is used more efficiently, because only peaks containing the desired products are collected. Unfortunately, mass peaks are broader than UV peaks, and therefore, fractions contaminated by a closely following peak are often collected. This can be avoided if the collection in preparative LC/MS occurs by a logical AND combination of UV- and mass-triggered collection. The success of this optimal collection mode is shown for three examples.
Thermally induced budding of phospholipid vesicles — a discontinuous process (1993)

The process of thermally induced budding is studied for DMPC vesicles. With increasing temperature a pear shaped vesicle changes discontinuously into a pair of spherical vesicles connected by a narrow neck. In the course of the cooling process a thermal hysteresis in a stepwise manner is observed. The size of the daughter vesicle diminishes by repeated widening and narrowing of the neck until it finally remains open and the vesicle attains the pear shape again. These experimental findings are difficult to rationalize within the bare bilayer couple model of phospholipid vesicles. To model the discontinuous behavior in the heating process the expansion of the elastic energy of the bilayer up to the third order in the area difference of the two monolayers is empirically introduced. The van der Waals forces between moter and daughter vesicles are shown to be responsible for the repeated widening and narrowing of the neck in the cooling process.
Effect of the nuclear factors EmBP1 and viviparous1 on the transcription of the Em gene in HeLa nuclear extracts. (1997)

Templates constructed from the wheat Em and maize rab28 promoters are efficiently and accurately transcribed in the well-characterized cell-free transcription system prepared from HeLa nuclei. Deletion analysis of the Em promoter indicates that a G-box (CACGTG) element (Em1b) is required for transcription. USF, a Myc transcription factor in HeLa nuclear extracts, activates transcription by binding to Em1b, as shown by the ability of an antibody raised against USF to inhibit transcription and to interfere with Em1b complex formation in an electrophoretic mobility shift assay. The addition of the recombinant Viviparous1 protein from maize to HeLa nuclear extracts specifically stimulated transcription of the Em promoter but was dependent on the presence of USF in the extract. In USF-depleted extracts, the addition of recombinant EmBP1, a basic leucine zipper transcription factor from wheat, activated transcription through Em1b as well as from a similar G-box in the adenovirus major late promoter. Our study demonstrates that the basic transcriptional apparatus in HeLa nuclear extract supports transcription from plant promoters and can be used to assay the function of certain plant nuclear proteins, thereby helping to determine their effects on transcription.
A 3-dimensional representation for fast rendering of complex scenes (1980)

Hierarchical representations of 3-dimensional objects are both time and space efficient. They typically consist of trees whose branches represent bounding volumes and whose terminal nodes represent primitive object elements (usually polygons). This paper describes a method whereby the object space is represented entirely by a hierarchical data structure consisting of bounding volumes, with no other form of representation. This homogencity allows the visible surface rendering to be performed simply and efficiently.
Real‐Time Ventricular Fibrillation Amplitude‐Spectral Area Analysis to Guide Timing of Shock Delivery Improves Defibrillation Efficacy During Cardiopulmonary Resuscitation in Swine (2017)

The AD protocol improved the time precision for shock delivery, resulting in less shock burden and less postresuscitation myocardial dysfunction, potentially improving survival compared with time-fixed, guidelines-driven, shock delivery protocols.
Broadband doa estimation using convolutional neural networks trained with noise signals (2017)

A convolution neural network (CNN) based classification method for broadband DOA estimation is proposed, where the phase component of the short-time Fourier transform coefficients of the received microphone signals are directly fed into the CNN and the features required for DOA estimation are learned during training. Since only the phase component of the input is used, the CNN can be trained with synthesized noise signals, thereby making the preparation of the training data set easier compared to using speech signals. Through experimental evaluation, the ability of the proposed noise trained CNN framework to generalize to speech sources is demonstrated. In addition, the robustness of the system to noise, small perturbations in microphone positions, as well as its ability to adapt to different acoustic conditions is investigated using experiments with simulated and real data.
YTRF is the conserved internalization signal of the transferrin receptor, and a second YTRF signal at position 31-34 enhances endocytosis. (1993)

By functional analysis of mutant human transferrin receptors (TR) expressed in chicken embryo fibroblasts, we previously identified a tetrapeptide sequence, Y20TRF23, within the 61-residue cytoplasmic tail as the signal for high-efficiency endocytosis (Collawn, J. F., Stangel, M., Kuhn, L. A., Esekogwu, V., Jing, S., Trowbridge, I.S., and Tainer, J.A. (1990) Cell 63, 1061-1072). It has been inferred from other studies, however, that the TR internalization signal was localized to a much larger region, residues 7 through 26 (Girones, N., Alvarez, E., Seth, A., Lin, I-M., Latour, D.A., and Davis, R.J. (1991) J. Biol. Chem. 266, 19006-19012). Additionally, Tyr20 was reported to not be conserved in the Chinese hamster cytoplasmic tail sequence (Alvarez, E., Girones, N., and Davis, R.J. (1990) Biochem. J. 267, 31-35). In the studies reported here, we examined the effect of insertion of an extra copy of a YTRF sequence at three different locations within the human TR cytoplasmic domain and show that the insertion of another YTRF signal at position 31-34 in the wild-type TR, but not the other two locations, increases the rate of endocytosis 2-fold. Furthermore, introduction of YTRF at position 31-34 in an internalization-defective mutant receptor restores endocytosis to wild-type levels, indicating that YTRF signals at either positions 20-23 or 31-34 are necessary and sufficient to promote TR internalization and function in an independent and additive manner. We also report the complete primary structure of the Chinese hamster TR deduced from its cDNA sequence and show that the Tyr20 as well as the complete YTRF motif is conserved.
Contact System: A Vascular Biology Modulator With Anticoagulant, Profibrinolytic, Antiadhesive, and Proinflammatory Attributes (1997)

physiologic reactions of vascular biology. Last, this presentation will point to possible new therapeutic strategies to treat HE KALLIKREIN-KININ system was first recognized as a plasma and tissue proteolytic system responsible for the liberation of the vasoactive, proinflammatory media-various diseases arising out of the knowledge of this system in physiologic and pathophysiologic states. tor, bradykinin (BK). 1 BK, a nonapeptide released from kininogens by kallikreins, could reproduce many of the charac-
Service trading and mediation in distributed computing systems (2002)

The increased availability of global communication infrastructures allows providers and users of various application services to cooperate in nearly unlimited geographic scopes. Problems of heterogeneity and scale have motivated specific standardisation activities for client/server "trading" or service "mediation" components. Motivated by current limitations of the emerging ODP (Open Distributed Processing) trader, this paper argues for a broader concept of general service "mediation" as more appropriate for realistic open distributed environments. The proposed mediation concept addresses some of the heterogeneity and flexibility requirements of open service co-operation by a uniform "Service Interface Description Language" (SIDL). The goal is to support distributed application development for a "Common Open Service Market" (COSM) by facilitating flexible service selection and client/server interaction. The paper also presents basic components of a generalised trading and mediation architecture as well as the status of a prototype implementation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
ChemInform Abstract: SYNTHESIS AND ANTIINFLAMMATORY ACTIVITY OF SOME 2‐(4′‐CARBOXYMETHYL‐3′‐METHYL‐5′‐OXO‐2′‐PYRAZOLIN‐1′‐YL)‐4‐ARYLTHIAZOLES AND 2‐(4′‐CARBOXYMETHYL‐3′‐METHYL‐5′‐OXO‐2′‐PYRAZOLIN‐1′‐YL)BENZOTHIAZOLES (1983)

Abstract Die Titelverbindungen (III) bzw. (V) werden aus den Hydrazinen (I) bzw. (IV) und dem Diester (II) synthetisiert.