gebhart/patrepeval huggingface dataset:
Putative Alleles for Increased Yield from Soybean Plant Introductions (2004)

Improving seed yield of soybean [ Glycine max (L.) Merr.] is an important breeding goal. The objective of this study was to evaluate two soybean PIs as sources of alleles for the enhancement of seed yield in North American cultivars. A population of 167 F 5 –derived lines was developed from a cross between ‘BSR 101’ and the experimental line LG82‐8379. BSR 101 has nine of 10 major ancestral lines contributing to the commercial gene pool of North America, while LG82‐8379 was selected from a cross between PI 68508 and FC 04007B. The F 5 –derived lines, divided into three sets based on maturity, were evaluated for 145 polymorphic simple sequence repeat (SSR) marker loci and for seed yield and other agronomic traits in 12 environments. Fifteen quantitative trait loci (QTL) were significantly [ P &lt; 0.05, likelihood of odds (LOD) &gt; 2.5] associated with seed yield in at least one set with two significant across all sets. For nine of the yield QTL, the LG82‐8379 alleles were associated with yield increases of 1.7 to 5.4% while the BSR 101 alleles increased yield 2.4 to 4.4% at six yield QTL. Four yield QTL were associated with significant changes in R8, eight with plant height, and three with seed protein concentration. Additional QTL were identified for R8, plant height, lodging, and seed protein and oil concentration. These results indicate that soybean PIs have the genetic potential for improving seed yield of U.S. soybean cultivars.
Inhibition of Histone Deacetylases: A Pharmacological Approach to the Treatment of Non-Cancer Disorders (2009)

The dynamics of gene expression are regulated by histone acetylases (HATs) and histone deacetylases (HDACs) that control the acetylation state of lysine side chains of the histone proteins of chromatin. The catalytic activity of these two enzymes remodels chromatin to control gene expression without altering gene sequence. Treatment of cancer has been the primary target for the clinical development of HDAC inhibitors, culminating in approval for the first HDAC inhibitor for the treatment of cutaneous T cell lymphoma. Beyond cancer, HDAC inhibition has potential for the treatment of many other diseases. The HDAC inhibitors phenylbutyric acid, valproic acid, and suberoylanilide hydroxamic acid (SAHA) have been shown to correct errant gene expression, ameliorate the progression of disease, and restore absent synthetic or metabolic activities for a diverse group of non-cancer disorders. These benefits have been found in patients with sickle cell anemia, HIV, and cystic fibrosis. In vitro and in vivo models of spinal muscular atrophy, muscular dystrophy, and neurodegenerative, and inflammatory disorders also show response to HDAC inhibitors. This review examines the application of HDAC inhibition as a treatment for a wide-range of non-cancer disorders, many of which are rare diseases that urgently need therapy. Inhibition of the HDACs has general potential as a pharmacological epigenetic approach for gene therapy.
Cellular uptake and spread of the cell‐permeable peptide penetratin in adult rat brain (2000)

Investigation of normal and pathological diseases of the central nervous system (CNS) has been hampered by the inability to effectively manipulate protein function in vivo. In order to address this important topic, we have evaluated the ability of penetratin, a novel cell-permeable peptide consisting of a 16-amino acid sequence derived from a Drosophila homeodomain protein, to act as a carrier system to introduce a cargo into brain cells. Fluorescently tagged penetratin was injected directly into rat brain, either into the striatum or the lateral ventricles, and rats were perfusion-fixed 24 h later in order to assess the brain response to the peptide. Immunohistochemistry following intrastriatal injection showed that injection of 10 microg penetratin caused neurotoxic cell death and triggered recruitment of inflammatory cells in a dose-dependent fashion. Doses of 1 microg or less resulted in reduced toxicity and recruitment of inflammatory cells, but interestingly, there was some spread of the penetratin. Injections of an inactive peptide sequence, derived from the same homeodomain, caused little toxicity but could still, however, trigger an inflammatory response. Intraventricular injections showed extensive inflammatory cell recruitment but minimal spread of either peptide. These results suggest that a dose of 1 microg of penetratin peptide is suitable for directing agents to small, discrete areas of the brain and as such is an interesting new system for analysing CNS function.
Tunable Reflective Spatial Heterodyne Spectrometer: A Technique for High Resolving Power, Wide Field Of View Observation Of Diffuse Emission Line Sources (2015)

The purpose of this dissertation is to discuss the need for new technology in broadband high-resolution spectroscopy based on the emerging technique of Spatial Heterodyne Spectroscopy (SHS) and to propose new solutions that should enhance and generalize this technology to other fields. Spectroscopy is a proven tool for determining compositional and other properties of remote objects. Narrow band imaging and low resolving spectroscopic measurements provide information about composition, photochemical evolution, energy distribution and density. The extension to high resolving power provides further access to temperature, velocity, isotopic ratios, separation of blended sources, and opacity effects. In current high resolving power devices, the drawback of high-resolution spectroscopy is bound to the instrumental limitations of lower throughput, the necessity of small entrance apertures, sensitivity, field of view, and large physical instrumental size. These limitations quickly become handicapping for observation of faint and/or extended targets and for spacecraft encounters. A technique with promise for the study of faint and extended sources at high resolving power is the reflective format of the Spatial Heterodyne Spectrometer (SHS). SHS instruments are compact and naturally tailored for both high étendue (defined in section 2.2.5) and high resolving power. In contrast, to achieve similar spectral grasp, grating spectrometers require large telescopes. For reference, SHS is a cyclical interferometer that produces Fizeau fringe pattern for all other wavelengths except the tuned wavelength. The large étendue obtained by SHS instruments makes them ideal for observations of extended, low surface brightness, isolated emission line sources, while their intrinsically high spectral resolution enables one to study the dynamical and physical properties described above. This document contains four chapters. Chapter 1, introduces a class of scientific targets that formerly have not been extensively observed due to absence of technical capabilities in current apparatus. We will introduce the concept of Special Heterodyne Spectrometers and address how it can fill the gap. Chapter 2 reports on the development of a new mathematical frame work for the Reflective SHS. Chapter 3 provides the details of the design and construction of a Tunable Reflective SHS at both UC Davis laboratory and Mt. Hamilton, Lick Observatory, CA. And chapter 4 contains an overview of the prospects of SHS instruments in future.
Quorum-Sensing Genes in Pseudomonas aeruginosa Biofilms: Their Role and Expression Patterns (2001)

Acylated homoserine lactone molecules are used by a number of gram-negative bacteria to regulate cell density-dependent gene expression by a mechanism known as quorum sensing (QS). In Pseudomonas aeruginosa, QS or cell-to-cell signaling controls expression of a number of virulence factors, as well as biofilm differentiation. In this study, we investigated the role played by the las and rhl QS systems during the early stages of static biofilm formation when cells are adhering to a surface and forming microcolonies. These studies revealed a marked difference in biofilm formation between the PAO1 parent and the QS mutants when glucose, but not citrate, was used as the sole carbon source. To further elucidate the contribution of lasI and rhlI to biofilm maturation, we utilized fusions to unstable green fluorescent protein in concert with confocal microscopy to perform real-time temporal and spatial studies of these genes in a flowing environment. During the course of 8-day biofilm development, lasI expression was found to progressively decrease over time. Conversely, rhlI expression remained steady throughout biofilm development but occurred in a lower percentage of cells. Spatial analysis revealed that lasI and rhlI were maximally expressed in cells located at the substratum and that expression decreased with increasing biofilm height. Because QS was shown previously to be involved in biofilm differentiation, these findings have important implications for the design of biofilm prevention and eradication strategies.
NR 51. Cereal substitution by molasses suplementation on grazing bulls at fattening stage and its effect on weight gain (1997)

In order to substitute corn and rice with molasses-urea 2 % in grazing bulls at fattening stage an experiment was accomplished in a randomizeds block design with 10 replications and three treatments. Statistical analysis was ran utilizaing the program IRRISAT. Results demonstrated the technical-economical feasibility of utilization molasses-urea 2 % in sustitution of feed grains with an economic profit of Bs 157/bull/day.
An efficient floating-point multiplier for digital signal processors (2014)

The floating-point multiplication is one of the most basic and frequent digital signal processing operations, and its accuracy and throughput greatly decide the overall accuracy and throughput of the digital signal processors. Based on vectorizing a conventional double precision multiplier, we propose a multiple precision floating-point multiplier. It supports either one double precision multiplication for high accuracy or two parallel single precision multiplications for high throughput. The evaluation results show that the proposed multiplier is suitable for embedded DSPs. It consumes 8.9% less area than two single precision multipliers. Compared the configuration with a single precision multiplier and a double precision multiplier, the proposed multiplier consumes 30.1% less area.
Circulating antibodies to guanosine in systemic lupus erythematosus: correlation with nephritis and polyserositis by acute and longitudinal analyses (2001)

Systemic lupus erythematosus (SLE) is characterized by autoantibodies, including antibodies to the nucleosides of DNA. Guanosine is the most immunogenic nucleoside. In this study serum antiguanosine antibody levels were compared with disease activity, determined by their SLEDI score, in 86 patients with SLE. Sera from these patients were tested, by ELISA, for autoantibodies to guanosine, single-stranded DNA (ssDNA), and double-stranded DNA (dsDNA). Anti-double-stranded DNA levels were also measured by RIA. Resultant values from these assays were correlated with SLE disease activity, and compared with specific features of SLE. The strongest correlation was higher levels of antiguanosine antibodies in patients with active lupus nephritis and polyserositis compared to patients with inactive disease (P < 0.0001). Antiguanosine levels also correlated with arthritis (P < 0.006), CNS lupus (P < 0.005), and hematologic manifestations of SLE (P < 0.002). To test the validity of this association in chronic SLE, serum antiguanosine antibodies were measured in patients with SLE at various phases of disease activity. Twelve patients with SLE had serum samples drawn at active, active-improved, and inactive phases over a 3-7 y period. Differences were significant for serum antiguanosine antibodies in the active group compared to the inactive group (P < 0.05) and the active vs the active-improved group (P < 0.02), unlike those for dsDNA and ssDNA by ELISA or RIA. Antiguanosine antibodies correlated more closely with disease activity in SLE patients in this longitudinal study than either anti-dsDNA or ssDNA antibodies. Thus, antibodies to guanosine correlated as well or better with disease activity than the other anti-DNA antibodies measured and should be considered to contribute to the pathology of SLE, especially lupus nephritis.
Hybrid 2D–0D MoS2–PbS Quantum Dot Photodetectors (2014)

A hybrid phototransistor consisting of colloidal PbS quantum dots and few layers of MoS2 (≥2 layers) is demonstrated. The hybrid benefits from tailored light absorption in the quantum dots throughout the visible/near infrared region, efficient charge-carrier separation at the p-n interface, and fast carrier transport through the MoS2 channel. It shows responsivity of up to 10(6) A W(-1) and backgate-dependent sensitivity.
NLRP1 polymorphisms in patients with asbestos-associated mesothelioma (2012)

Although NLRP3 SNPs was not involved in mesothelioma predisposition, these data proposed NLRP1 as a novel factor possibly involved in the development of mesothelioma.
Lipid peroxidation and disintegration of the cell membrane structure in cultures of rat lung fibroblasts treated with asbestos (1993)

Rat lung fibroblasts were cultured for 24 and 48 h with UICC standard reference asbestos samples of amosite, crocidolite or Canadian chrysotile at various concentrations, and thiobarbituric acid-reactive substances (TBARS) in the media and the cells were measured. Tests by the trypan blue dye-exclusion method showed that cell viability was 80 +/- 5% (mean +/- SD) and 71 +/- 6% when cultured for 48 h in the presence of 500 micrograms ml-1 of amosite and crocidolite, respectively, but was not affected by chrysotile. In cultures for 24 and 48 h, both chrysotile and crocidolite at > 250 micrograms ml-1 significantly increased TBARS in the medium, whereas amosite did so at > 500 micrograms ml-1. TBARS in the cells was not increased significantly by chrysotile at any concentration in the cultures for 24 and 48 h, but crocidolite at > 250 micrograms ml-1 significantly increased TBARS in the cells when cultured for 24 or 48 h. Although amosite at all concentrations tested did not increase significantly TBARS in the cells of the 24-h culture, it did increase TBARS significantly in the cells when cultured for 48 h at a concentration of 500 micrograms ml-1 amosite. The increases of TBARS in media of the cultures with asbestos were accompanied by increases of lactate dehydrogenase (LDH) activity in the media, indicating the leakage of the enzyme from the cell into the media. The activity of LDH and the amount of TBARS showed a good correlation with each other, with a significant correlation coefficient value of 0.655.(ABSTRACT TRUNCATED AT 250 WORDS)
A five-year analysis of botulinum toxin type A injections: some unusual features. (1991)

We analyzed patients treated during the past five years with botulinum toxin type A for strabismus and blepharospasm, reviewed our successes, failures, and unusual cases, and drew conclusions based on these treatments. Thirty-seven percent of the strabismus patients were cured, but many patients who were outside the strict definitions, still believed that they were significantly improved. A prominent feature in the treatment of strabismus was variability. Frequently, patients expected to do poorly had encouraging results. One permanent overcorrection occurred, and it converted an esotopic patient into an exotropic one with diplopia. This has persisted for 2.5 years and is the longest reported overcorrection to our knowledge. Our results indicate that larger doses of botulinum toxin produce longer spasm-free intervals in the treatment of blepharospasm. One patient receiving injections for her blepharospasm discovered that its cause was her sedative medication. This is the first reported case of a benzodiazepine inducing blepharospasm to our knowledge.
From Flexible to Constrained Tris(tetraamine) Ligands: Synthesis, Acid–Base Properties, and Structural Effect on the Coordination Process with Nucleotides (2010)

Abstract This work presents the synthesis and characterization of L2 and L3 , which formed with the previously described ligand L1 a family of three tris(tetraamine) ligands based on a mesitylenyl spacer. The trimeric ligands differ from one to the other by the nature of their tetraamine moieties: triethylenetetraamine ( L1 ), cyclen ( L2 ), and constrained cyclen ( L3 ). The impact of the rigidification of the structure from L1 to L3 on the acid–base properties is investigated, and the behavior of the ligands towards triphosphate (TriP), adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) anion receptors is explored by potentiometric investigations. To characterize and understand the driving forces implicated in the supramolecular assemblies, results are supported by 1 H and 31 P NMR measurements performed over a wide pH range. ATP appears as the most complexed anion and particular attention is given to comparison with its inorganic triphosphate analogue to highlight the contribution of π‐stacking interactions. Results unambiguously show the various coordination scheme induced by the structure of the ligands.
CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells (2015)

Direct visualization of genomic loci in the 3D nucleus is important for understanding the spatial organization of the genome and its association with gene expression. Various DNA FISH methods have been developed in the past decades, all involving denaturing dsDNA and hybridizing fluorescent nucleic acid probes. Here we report a novel approach that uses in vitro constituted nuclease-deficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated caspase 9 (Cas9) complexes as probes to label sequence-specific genomic loci fluorescently without global DNA denaturation (Cas9-mediated fluorescence in situ hybridization, CASFISH). Using fluorescently labeled nuclease-deficient Cas9 (dCas9) protein assembled with various single-guide RNA (sgRNA), we demonstrated rapid and robust labeling of repetitive DNA elements in pericentromere, centromere, G-rich telomere, and coding gene loci. Assembling dCas9 with an array of sgRNAs tiling arbitrary target loci, we were able to visualize nonrepetitive genomic sequences. The dCas9/sgRNA binary complex is stable and binds its target DNA with high affinity, allowing sequential or simultaneous probing of multiple targets. CASFISH assays using differently colored dCas9/sgRNA complexes allow multicolor labeling of target loci in cells. In addition, the CASFISH assay is remarkably rapid under optimal conditions and is applicable for detection in primary tissue sections. This rapid, robust, less disruptive, and cost-effective technology adds a valuable tool for basic research and genetic diagnosis.
Repeat-Until-Success: Non-deterministic decomposition of single-qubit unitaries (2014)

We present a decomposition technique that uses non-deterministic circuits to approximate an arbitrary single-qubit unitary to within distance $\epsilon$ and requires significantly fewer non-Clifford gates than existing techniques. We develop ``Repeat-Until-Success" (RUS) circuits and characterize unitaries that can be exactly represented as an RUS circuit. Our RUS circuits operate by conditioning on a given measurement outcome and using only a small number of non-Clifford gates and ancilla qubits. We construct an algorithm based on RUS circuits that approximates an arbitrary single-qubit $Z$-axis rotation to within distance $\epsilon$, where the number of $T$ gates scales as $1.26\log_2(1/\epsilon) - 3.53$, an improvement of roughly three-fold over state-of-the-art techniques. We then extend our algorithm and show that a scaling of $2.4\log_2(1/\epsilon) - 3.28$ can be achieved for arbitrary unitaries and a small range of $\epsilon$, which is roughly twice as good as optimal deterministic decomposition methods.
Observation of longitudinal spin-Seebeck effect in magnetic insulators (2010)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Ken-ichi Uchida, Hiroto Adachi, Takeru Ota, Hiroyasu Nakayama, Sadamichi Maekawa, Eiji Saitoh; Observation of longitudinal spin-Seebeck effect in magnetic insulators. Appl. Phys. Lett. 25 October 2010; 97 (17): 172505. https://doi.org/10.1063/1.3507386 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioApplied Physics Letters Search Advanced Search |Citation Search
Correlation of the adipocyte-derived protein adiponectin with insulin resistance index and serum high-density lipoprotein-cholesterol, independent of body mass index, in the Japanese population (2002)

Adiponectin, which is secreted specifically by adipose tissue, has been shown to act as an anti-atherosclerotic protein by direct effects on endothelial cells. Clinical studies have shown that adiponectin levels are lower in individuals with obesity, diabetes and coronary artery disease. The present study investigated relationships between serum adiponectin levels and body mass index (BMI), blood pressure, insulin resistance index, lipid profile, uric acid and high-sensitivity C-reactive protein levels in a large number of Japanese subjects not taking any medication for metabolic disease and without severe illness (705 men and 262 women; age 30-65 years; BMI 22.5+/-2.9 kg/m(2)). The serum adiponectin concentration was measured by ELISA, without a protein-denaturing step. The insulin resistance index was assessed by homoeostasis model assessment (HOMA-IR). The serum concentration of adiponectin in women (13.5+/-7.9 microg/ml) was significantly higher than that in men (7.2+/-4.6 microg/ml). The serum adiponectin level was negatively correlated with BMI, systolic blood pressure, diastolic blood pressure, fasting plasma glucose, insulin, HOMA-IR, total cholesterol, triacylglycerols, low-density lipoprotein (LDL)-cholesterol and uric acid, and positively correlated with high-density lipoprotein (HDL)-cholesterol. The correlations between serum adiponectin level and insulin, HOMA-IR, triacylglycerols, HDL-cholesterol, LDL-cholesterol and uric acid were significant even after adjustment for age, sex and BMI. Stepwise multiple regression analysis revealed that HDL-cholesterol, sex, BMI and HOMA-IR were independently correlated with the serum adiponectin level (R(2)=0.377). These findings suggest that the serum adiponectin level is negatively correlated with HOMA-IR and positively correlated with HDL-cholesterol, independent of age, sex and BMI, in the Japanese population.
Diaspirin Cross-Linked Hemoglobin (DCLHb) in the Treatment of Severe Traumatic Hemorrhagic Shock<SUBTITLE>A Randomized Controlled Efficacy Trial</SUBTITLE> (1999)

Mortality was higher for patients treated with DCLHb. Although further analysis should investigate whether the mortality difference was solely due to a direct treatment effect or to other factors, DCLHb does not appear to be an effective resuscitation fluid.
INETPhone: Telephone Services and Servers on Internet (1995)

INETPhone is a true telephone service through the Internet. It integrates the local telephone networks and the Internet using INETPhone servers. Thus a long distance call can be split into two local calls and an Internet connection, which is transparent to end users. Such a phone service through Internet will be a major step towards integrated services on Internet. In order to support the INETPhone and lay down the ground rules of the service, a scheme of open partnership is proposed, so that the entire Internet community can have the equal opportunity and benefits from the INETPhone service.