gebhart/patrepeval huggingface dataset:
Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen (1996)

A human macrophage calcium-dependent (C-type) lectin cDNA clone was obtained from a library derived from IL-2-treated peripheral blood monocytes. The cDNA cloning was based on the structural homology to hepatic asialoglycoprotein receptors. The nucleotide sequence of this cDNA clone was homologous to those of the galactose- and N-acetylgalactosamine-specific C-type macrophage lectins of rodents. In the putative carbohydrate recognition domain, deduced amino acid sequence revealed 60 and 63% homology to galactose- and N-acetylgalactosamine-specific C-type macrophage lectins of mice and rats, respectively. The cDNA clone was ligated into a mammalian expression vector and transfected into COS-1 cells. In the lysates of these cells, an MR 38,000 component, which bound to galactose-Sepharose, was identified after electrophoretic separation by its interaction with polyclonal antisera against synthetic polypeptides representing a portion of the carbohydrate recognition domain. The carbohydrate-binding specificity of the recombinant macrophage lectin was investigated by comparing elution profiles of various glycopeptides having defined carbohydrate structures from immobilized macrophage lectins. When N-terminal octapeptides from human glycophorin A that bore NeuAc alpha 2-3 Gal beta 1-3 (NeuAc alpha 2-6) GalNAc and its sequentially deglycosylated derivatives were compared, glycopeptides carrying three constitutive GalNAc-Ser/Thr (Tn Ag) strongly bound to the recombinant human macrophage lectin. This is the first study to demonstrate that human macrophage cell surface lectin recognizes Tn Ag, a well-known human carcinoma-associated epitope.
Control of microbial souring by nitrate, nitrite or glutaraldehyde injection in a sandstone column (1996)

Microbial souring (production of hydrogen sulfide by sulfate-reducing bacteria, SRB) in crushed Berea sandstone columns with oil field-produced water consortia incubated at 60~ was inhibited by the addition of nitrate (NO3) or nitrite (NO2). Added nitrate (as nitrogen) at a concentration of 0.71 mM resulted in the production of 0.57-0.71 mM nitrite by the native microbial population present during souring and suppressed sulfate reduction to below detection limits. Nitrate added at 0.36 mM did not inhibit active souring but was enough to maintain inhibition if the column had been previously treated with 0.71 mM or greater. Continuous addition of 0.71-0.86 mM nitrite also completely inhibited souring in the column. Pulses of nitrite were more effective than the same amount of nitrite added continuously. Nitrite was more effective at inhibiting souring than was glutaraldehyde, and SRB recovery was delayed longer with nitrite than with glutaraldehyde. It was hypothesized that glutaraldehyde killed SRB while nitrite provided a long-term inhibition without cell death. Removal of nitrate after as long as 3 months of continuous addition allowed SRB in a biofilm to return to their previous level of activity. Inhibition was achieved with much lower levels of nitrate and nitrite, and at higher temperatures, than noted by other researchers.
Do We Encourage Health or Illness? A Survey of Exercise Rehabilitation Practices for Patients in Australian Renal Units (2006)

Background: People with end stage renal disease (ESRD) have been reported as having low levels of physical activity. Sedentary behaviours increase illness risks which may lead to burdens on the public health system which include costs of medical care. Research has established that exercise is reported to improve general health and wellbeing. Benefits include better aerobic tolerance, maintenance and improvement in physical function and capacity, and improvement in self-concept and well-being. These same improvements also occur in an exercising ESRD population, even though the improvements might be of less magnitude. Renal unit staff can have a major impact on patients with ESRD by actively promoting exercise. Purpose: The study surveyed renal units throughout Australia, to obtain information on exercise rehabilitation practices within these units. Method: This was a descriptive single cross sectional study of dialysis units within Australia. Each unit was asked what exercise rehabilitation practices were conducted in their units at a basic, intermediate and advanced level. The survey questionnaire was adapted from the Unit Self-Assessment Manual for Renal Rehabilitation (The Life Options Rehabilitation Advisory Council [LORAC] 1998). Results: Twenty-two units responded, a 52.38% return rate. On average, only 9% of the content of the education programs was related to exercise practices, and only 9% of units had organized fitness programs. Most of the education programs were related to diet and fluids, dialysis therapies, and vascular access care. 50% of units reported they regularly referred patients to occupational therapy and physiotherapy but the outcome of these referrals in terms of physical activity is unknown. Comments demonstrated that staff were aware of the importance of exercise, however many comments related to unmotivated patients and lack of resources (staff and finances) to support physical activity programs. Conclusion: The results of this study suggest that there is a real need to actively develop physical activity rehabilitation programs within Australian dialysis units. With the large amount of literature now available reporting the benefits of exercise in those with ESRD, an important consideration for renal physicians and nurses is their ‘duty of care’ to their patients to promote physical activity.
Synthetic lethality: a framework for the development of wiser cancer therapeutics (2009)

The challenge in medical oncology has always been to identify compounds that will kill, or at least tame, cancer cells while leaving normal cells unscathed. Most chemotherapeutic agents in use today were selected primarily for their ability to kill rapidly dividing cancer cells grown in cell culture and in mice, with their selectivity determined empirically during subsequent animal and human testing. Unfortunately, most of the drugs developed in this way have relatively low therapeutic indices (low toxic dose relative to the therapeutic dose). Recent advances in genomics are leading to a more complete picture of the range of mutations, both driver and passenger, present in human cancers. Synthetic lethality provides a conceptual framework for using this information to arrive at drugs that will preferentially kill cancer cells relative to normal cells. It also provides a possible way to tackle 'undruggable' targets. Two genes are synthetically lethal if mutation of either gene alone is compatible with viability but simultaneous mutation of both genes leads to death. If one is a cancer-relevant gene, the task is to discover its synthetic lethal interactors, because targeting these would theoretically kill cancer cells mutant in the cancer-relevant gene while sparing cells with a normal copy of that gene. All cancer drugs in use today, including conventional cytotoxic agents and newer 'targeted' agents, target molecules that are present in both normal cells and cancer cells. Their therapeutic indices almost certainly relate to synthetic lethal interactions, even if those interactions are often poorly understood. Recent technical advances enable unbiased screens for synthetic lethal interactors to be undertaken in human cancer cells. These approaches will hopefully facilitate the discovery of safer, more efficacious anticancer drugs that exploit vulnerabilities that are unique to cancer cells by virtue of the mutations they have accrued during tumor progression.
Role of Computed Tomography Coronary Angiography in the Detection of Vulnerable Plaque, Where Does it Stand Among Others? (2013)

Recently, there has been a growing interest in identification of coronary “vulnerable plaques” that are prone to rupture; this potentially would help identify patients with higher risk of development of cardiac events. Recent advances in cardiac imaging modalities have been successful in studying various plaque vulnerability features to variable degrees, strengths and limitations. Computed Tomography Coronary Angiography (CTCA) has gained an increasing popularity in studying plaque anatomy, morphology and composition by the virtue of its widespread availability and non-invasiveness. CTCA has been validated against histology and IVUS with reasonable correlation; moreover, some follow-up studies have shown a significant association to the development of acute coronary syndromes. Nevertheless, attention should be paid to the whole patient big picture that includes other factors operating on other extra-coronary axes that involve inflammation, immunity, coagulation and neuroendocrine systems.
Glass-Glass Wafer Bonding for Microfluidic Devices (2008)

The AML AWB04 wafer bonding platform has been used to develop glass – glass bonding processes for both quartz and Pyrex substrates. This allows the accurate wafer to wafer alignment and bonding of two microfluidic (or any other glass device) wafers. In the case of Quartz, the process is also useful in the field of SAW (Surface Acoustic Wave) device fabrication. Although there are simpler and cheaper ways of creating micro fluidic devices (e.g adhesive bonded glass wafers, or all polymer devices), Pyrex is often required as the material is qualified for use in pharmaceutical and medical industries. The transmission properties of glass are also often needed where optical sensing and measurement is required. Pyrex is often needed for chemical compatibility, and is commonly used for glassware. Using the AML equipment, Pyrex and Quartz devices can be sealed at the wafer level with placement accuracies approaching ±1μm. The bonding process can also seal vacuum cavities, or seal controlled atmosphere cavities (e.g. reference cavities for optical absorption measurements). It is sometimes important to maintain precise glass micromachining dimensions and therefore it is necessary to bond at a temperature substantially less than the strain point of the glass: the AML equipment and processes can achieve this. The sealing strength is sufficient to withstand high pressure fluidic applications.
Synthesis and photovoltaic behaviors of narrow‐band‐gap π‐conjugated polymers composed of dialkoxybenzodithiophene‐ and thiophene‐based fused aromatic rings (2011)

Abstract π‐Conjugated polymers, PBDT‐CNETT and PBDT‐CNECPDT , were prepared by the Stille cross‐coupling polymerization. Optical and thermal properties of the obtained polymers were investigated by UV–vis spectroscopy and thermogravimetric analysis. PBDT‐CNETT and PBDT‐CNECPDT exhibited very narrow band gaps of 1.39 and 1.13 eV, respectively. Highest occupied molecular orbital energy levels estimated by surface analyzer were −5.17 and −5.11 eV for PBDT‐CNETT and PBDT‐CNECPDT , respectively. The solar cells based on these polymers were evaluated with the cell configuration of ITO/PEDOT‐PSS/polymer:PC 61 BH/LiF/Al. The power conversion efficiencies of the solar cells were estimated to be 1.57 and 0.16% for PBDT‐CNETT and PBDT‐CNECPDT , respectively. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Teplizumab therapy for type 1 diabetes (2010)

In Phase I/II randomized control trials, in patients with new onset T1D, teplizumab slowed the rate of loss of beta-cell function over 2 years of follow-up. Treated patients had better glycemic control and lower insulin requirements. Adverse events so far are mild and of limited duration. Phase III clinical trials are underway to confirm these results and to determine if two courses of drug have greater efficacy in arresting loss of beta-cell function.
A 71GHz RF energy harvesting tag with 8% efficiency for wireless temperature sensors in 65nm CMOS (2013)

This paper presents the first monolithically integrated RF-power harvesting 71 GHz wireless temperature sensor node in 65nm CMOS technology, containing a monopole antenna, a 71 GHz RF power harvesting unit with storage capacitor array, an End-of-Burst monitor, a temperature sensor and an ultra-low-power transmitter at 79 GHz. At 71 GHz, the RF to DC converter achieves a power conversion efficiency of 8% for 5 dBm input power.
MicroRNAs Regulate Brain Morphogenesis in Zebrafish (2005)

MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZdicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects in MZdicer mutants, revealing essential roles for miRNAs during morphogenesis.
Substituted thiopyrano[2,3,4-c,d]indoles as potent, selective, and orally active inhibitors of 5-lipoxygenase. Synthesis and biological evaluation of L-691,816 (1993)

Thiopyrano[2,3,4-c,d]indoles are a new class of 5-lipoxygenase (5-LO) inhibitors. SAR studies have demonstrated that the thiopyran ring, the 5-phenylpyridine substituent, and an acidic functional group on a four-carbon C-2 side chain are all required for optimal inhibitor potency. In contrast, the indolic nitrogen may be substituted with a variety of lipophilic groups. As a result of the SAR investigation, 44 (L-691,816; 5-[3-[1-(4-chlorobenzyl)-4-methyl-6-[(5-phenylpyridin-2-yl)methoxy ]- 4,5-dihydro-1H-thiopyrano[2,3,4-c,d]indol-2-yl]-2,2-dimethylpro pyl]-1H- tetrazole) has been identified as a potent inhibitor of the 5-LO reaction both in vitro and in a range of in vivo models. Compound 44 inhibits 5-HPETE production by both rat and human 5-LO and LTB4 synthesis in human PMN leukocytes (IC50s 16, 75, and 10 nM, respectively). The mechanism of inhibition of 5-LO activity by compound 44 appears to involve the formation of a reversible deadend complex with the enzyme and does not involve reduction of the nonheme iron of 5-LO. Compound 44 is highly selective for 5-LO when compared to the inhibition of human FLAP, porcine 12-LO, and also ram seminal vesicle cyclooxygenase. In addition, 44 is orally active in a rat pleurisy model (inhibition of LTB4, ED50 = 1.9 mg/kg; 8 h pretreatment) as well as in the hyperreactive rat model of antigen-induced dyspnea (ED50 = 0.1 mg/kg; 2-h pretreatment). Excellent functional activity was also observed in both the conscious allergic monkey and sheep models of asthma. In the latter case, the functional activity observed correlated with the inhibition of urinary LTE4 excretion.
Core promoter-selective RNA polymerase II transcription (2006)

The initiation of mRNA synthesis in eukaryotic cells is a complex and highly regulated process that requires the assembly of general transcription factors and RNAP II (RNA polymerase II; also abbreviated as Pol II) into a pre-initiation complex at the core promoter. The core promoter is defined as the minimal DNA region that is sufficient to direct low levels of activator-independent (basal) transcription by RNAP II in vitro. The core promoter typically extends approx. 40 bp up- and down-stream of the start site of transcription and can contain several distinct core promoter sequence elements. Core promoters in higher eukaryotes are highly diverse in structure, and each core promoter sequence element is only found in a subset of genes. So far, only TATA box and INR (initiator) element have been shown to be capable of directing accurate RNAP II transcription initiation independent of other core promoter elements. Computational analysis of metazoan genomes suggests that the prevalence of the TATA box has been overestimated in the past and that the majority of human genes are TATA-less. While TATA-mediated transcription initiation has been studied in great detail and is very well understood, very little is known about the factors and mechanisms involved in the function of the INR and other core promoter elements. Here we summarize our current understanding of the factors and mechanisms involved in core promoter-selective transcription and discuss possible pathways through which diversity in core promoter architecture might contribute to combinatorial gene regulation in metazoan cells.
Donor‐Induced Performance Tuning of Amorphous SrTiO3 Memristive Nanodevices: Multistate Resistive Switching and Mechanical Tunability (2015)

Metal–oxide valence‐change memristive devices are the key contenders for the development of multilevel nonvolatile analog memories and neuromorphic computing architectures. Reliable low energy performance and tunability of nonlinear resistive switching dynamics are essential to streamline the high‐density circuit level integration of these devices. Here, manipulation of room temperature‐synthesized defect chemistry is employed to enhance and tune the switching characteristics of high‐performance amorphous SrTiO 3 ( a ‐STO) memristors. Substitutional donor (Nb) doping with low concentrations in the a ‐STO oxide structure allows extensive improvements in energy requirements, stability, and controllability of the memristive performance, as well as field‐dependent multistate resistive switching. Evidence is presented that room temperature donor doping results in a modified insulator oxide where dislocation sites act as charge carrier modulators for low energy and multilevel operation. Finally, the performance of donor‐doped a ‐STO‐based memristive nanodevices is showcased, with the possibility of mechanical modulation of the nonlinear memristive characteristics of these devices demonstrated. These results highlight the potential of donor‐doped a ‐STO nanodevices for high‐density integration as analog memories and multifunctional alternative logic elements.
Characterization of Human CD8+ T Cells Reactive with Mycobacterium tuberculosis–infected Antigen-presenting Cells (1998)

Previous studies in murine models, including those using the beta2 microglobulin knockout mouse, have suggested an important role for CD8+ T cells in host defense to Mycobacterium tuberculosis (Mtb). At present, little is understood about these cells in the human immune response to tuberculosis. This report demonstrates the existence of human Mtb-reactive CD8+ T cells. These cells are present preferentially in persons infected with Mtb and produce interferon gamma in response to stimulation with Mtb-infected target cells. Recognition of Mtb-infected cells by these CD8+ T cells is restricted neither by the major histocompatibility complex (MHC) class I A, B, or C alleles nor by CD1, although it is inhibited by anti-MHC class I antibody. The Mtb-specific CD8+ T cells recognize an antigen which is generated in the proteasome, but which does not require transport through the Golgi-ER. The data suggest the possible use of nonpolymorphic MHC class Ib antigen presenting structures other than CD1.
Recent developments in Alzheimer's disease therapeutics (2009)

Alzheimer's disease is a devastating neurological disorder that affects more than 37 million people worldwide. The economic burden of Alzheimer's disease is massive; in the United States alone, the estimated direct and indirect annual cost of patient care is at least $100 billion. Current FDA-approved drugs for Alzheimer's disease do not prevent or reverse the disease, and provide only modest symptomatic benefits. Driven by the clear unmet medical need and a growing understanding of the molecular pathophysiology of Alzheimer's disease, the number of agents in development has increased dramatically in recent years. Truly *'disease-modifying' therapies that target the underlying mechanisms of Alzheimer's disease have now reached late stages of human clinical trials. Primary targets include beta-amyloid, whose presence and accumulation in the brain is thought to contribute to the development of Alzheimer's disease, and tau protein which, when hyperphosphorylated, results in the self-assembly of tangles of paired helical filaments also believed to be involved in the pathogenesis of Alzheimer's disease. In this review, we briefly discuss the current status of Alzheimer's disease therapies under study, as well the scientific context in which they have been developed.
Extração de macronutrientes pela fitomassa do capim-xaraés "xaraés" em função de doses de nitrogênio e potássio (2008)

O experimento foi conduzido em casa de vegetação, na Embrapa Arroz e Feijão, com objetivo de avaliar o efeito de doses de N e K na produção de massa seca e extração de macronutrientes pela fitomassa do capim xaraés. O delineamento utilizado foi bloco ao acaso, com esquema fatorial 4 x 4, sendo quatro doses de Nitrogênio: 0; 25, 50 e 100mg dm-3, e quatro doses de Potássio: 0; 25, 50 e 100mg dm-3, com três repetições. Foram realizados três cortes de avaliação da forrageira. Todos os macronutrientes sofreram variações significativas em função das doses de Nitrogênio e Potássio aplicadas. O efeito mais pronunciado sobre a extração dos macronutrientes foi proporcionado pelas doses de Potássio. A ordem decrescente de extração para o capim-xaraés, considerando extração máxima de cada nutriente, foi: N = K &gt; Ca &gt; Mg &gt; P &gt; S. Contudo, sugerem-se estudos dessas variáveis, em condições de campo, de modo a se obter mais informações sobre esse cultivar para que as estratégias de manejo da cultura sejam mais eficientes.
Evaluation of a Monoclonal Antibody-Based Test for Detection of Helicobacter pylori -Specific Antigen in Stool Samples from Mice (2004)

A test using monoclonal antibodies for detection of antigen in stool samples was compared with culture and histology for noninfected (n = 25), Helicobacter pylori-infected (n = 25), and Helicobacter felis-infected (n = 6) mice. Sensitivity and specificity were 96%. The monoclonal antibody-based test is therefore a noninvasive technique that is able to diagnose H. pylori infection in mice.