gebhart/patrepeval huggingface dataset:
Oil-Body-Membrane Proteins and Their Physiological Functions in Plants (2010)

Oilseeds accumulate a large amount of storage lipids, which are used as sources of carbon and energy for seed germination and seedling growth. The storage lipids are accumulated in oil bodies during seed maturation. Oil bodies in seeds are surrounded with three oil-body-membrane protein families, oleosins, caleosins and steroleosins. These proteins are plant-specific and much abundant in seeds. Here we show a unique function of oleosins in preventing fusion of oil bodies and maintaining seed germination. Reverse genetic analysis using oleosin-deficient mutants shows the inverse proportion of oil-body sizes to total oleosin contents. The double mutant ole1 ole2 with the lowest levels of oleosins has irregularly-enlarged oil bodies throughout the seed cells, and hardly germinates. Germination rates are positively associated with oleosin contents, suggesting that the defects of germination are related to the expansion of oil bodies due to oleosin deficiency. Interestingly, freezing treatment followed by imbibition at 4 °C inhibits seed germination of single mutants (ole1 and ole2), which germinate normally without freezing treatment. The freezing treatment accelerates the fusion of oil bodies and generates eccentric nuclei in ole1 seeds, which caused seed mortality. Taken together, our findings suggest that oleosins increase the viability of oilseeds by preventing abnormal fusion of oil bodies for overwintering. Knowledge of oleosin contributes a great deal to not only an insight into freezing tolerance of oilseeds, but also creating genetically modified plants for developing a bioenergy and biomass resource.
The Nasal Approach to Delivering Treatment for Brain Diseases: An Anatomic, Physiologic, and Delivery Technology Overview (2014)

The intricate pathophysiology of brain disorders, difficult access to the brain, and the complexity and high risks and costs of drug development represent major hurdles for improving therapies. Nose-to-brain drug transport offers an attractive alternative or addition to formulation-only strategies attempting to enhance drug penetration into the CNS. Although still a matter of controversy, many studies in animals claim direct nose-to-brain transport along the olfactory and trigeminal nerves, circumventing the traditional barriers to CNS entry. Some clinical trials in man also suggest nose-to-brain drug delivery, although definitive proof in man is lacking. This review focuses on new nasal delivery technologies designed to overcome inherent anatomical and physiological challenges and facilitate more efficient and targeted drug delivery for CNS disorders.
Large-scale synthesis of peptides (2000)

Recent advances in the areas of formulation and delivery have rekindled the interest of the pharmaceutical community in peptides as drug candidates, which, in turn, has provided a challenge to the peptide industry to develop efficient methods for the manufacture of relatively complex peptides on scales of up to metric tons per year. This article focuses on chemical synthesis approaches for peptides, and presents an overview of the methods available and in use currently, together with a discussion of scale-up strategies. Examples of the different methods are discussed, together with solutions to some specific problems encountered during scale-up development. Finally, an overview is presented of issues common to all manufacturing methods, i.e., methods used for the large-scale purification and isolation of final bulk products and regulatory considerations to be addressed during scale-up of processes to commercial levels.
Listening test materials for "A study of speaker adaptation for DNN-based speech synthesis" (2015)

The dataset contains the testing stimuli and listeners' MUSHRA test responses for the Interspeech 2015 paper, "A study of speaker adaptation for DNN-based speech synthesis". In this paper, we conduct an experimental analysis of speaker adaptation for Deep Neural Network (DNN) based speech synthesis at different levels. In particular, we augment a low-dimensional speaker-specific vector with linguistic features as input to represent speaker identity, perform model adaptation to scale the hidden activation weights, and perform a feature space transformation at the output layer to modify generated acoustic features. We systematically analyse the performance of each individual adaptation technique and that of their combinations. Experimental results confirm the adaptability of the DNN, and listening tests demonstrate that the DNN can achieve significantly better adaptation performance than the hidden Markov model (HMM) baseline in terms of naturalness and speaker similarity.
cDNA sequence of the human integrin beta 5 subunit. (1990)

A novel integrin receptor involved in cell adhesion to the matrix protein vitronectin has recently been described from a human lung epithelial-derived cell line (Cheresh, D. A., Smith, J. W., Cooper, H. M., and Quaranta, V. (1989) Cell 57, 59-69). This receptor has an alpha subunit that appears identical to the alpha v of the vitronectin receptor alpha v beta 3 expressed in melanoma and endothelial cells, but is complexed with a distinct beta subunit, beta 5. cDNA clones coding for beta 5 have been isolated and used to determine the mRNA and amino acid sequence of this new subunit. A 3.3-kilobase mRNA was found to code for a mature protein of 775 amino acid residues with a hydrophobic leader sequence of 24 amino acids. A 56% identity was found between the beta 5 and beta 3 protein sequences, making them the most closely related of the integrin beta subunits. Polymerase chain reaction abundance analysis revealed that alpha v and beta 5 mRNAs were found in seven very different cell lines, compared with beta 3 mRNA which was found in only three of the them, indicating that this new integrin receptor may be widely distributed.
Influence of Castration, Alone or Combined with Thymectomy, on the Development of Diabetes in the Nonobese Diabetic Mouse* (1991)

The nonobese diabetic (NOD) mouse is a recognized model for studying immunologically-mediated insulin-dependent diabetes mellitus. The disease appears with a greater preponderance in females than in males. Castration at weaning led to a significant increase in the prevalence of diabetes in NOD males, whereas a tendency to a decreased prevalence was observed in NOD females. Castration combined with thymectomy was found to further increase the prevalence of diabetes in NOD males, whereas in females castration reversed the effect of thymectomy. These results on changes in diabetes prevalence were corroborated by the degree of lymphocytic infiltration directed toward the pancreatic islets of Langerhans. Taken together these results indicate a direct relationship between the endocrine and immune systems, whereby orchidectomy has a deleterious effect on the immunopathogenesis of diabetes. In addition, we examined whether the distribution of lymphocyte subpopulations, mitogen reactivity, lymphokine production, and in vivo response to a thymus-dependent antigen, such as sheer red blood cell, were dependent or independent of the sex steroid environment.
Abscopal Effects: Case Report and Emerging Opportunities (2015)

The abscopal effect is a phenomenon observed in the treatment of metastatic cancer where localized irradiation of a particular tumor site causes a response in a site distant to the irradiated volume. The mechanisms of the abscopal effect are speculated to be of several origins, including distant effects on p53, elaboration of inflammatory agents including cytokines, and, most recently, secondary to immune mechanisms. In this case report, we present a rare report of a patient with hepatocellular carcinoma with lung metastases who, after receiving radiation treatment to the liver, had a treatment response in the liver and a complete response in the lung. Recent advances in the understanding of the primary role of immune-modulated cytotoxicity, especially with the success of immune checkpoint inhibitors, have the potential to turn the abscopal effect from a rare phenomenon into a tool to guide antineoplastic therapy and provide a new line of research.
Enhanced pressor response to increased CSF sodium concentration and to central ANG I in heterozygous α2Na+-K+-ATPase knockout mice (2009)

Intracerebroventricular (ICV) infusion of NaCl mimics the effects of a high-salt diet in salt-sensitive hypertension, raising the sodium concentration in the cerebrospinal fluid (CSF [Na]) and subsequently increasing the concentration of an endogenous ouabain-like substance (OLS) in the brain. The OLS, in turn, inhibits the brain Na(+)-K(+)-ATPase, causing increases in the activity of the brain renin-angiotensin system (RAS) and blood pressure. The Na(+)-K(+)-ATPase alpha (catalytic)-isoform(s) that mediates the pressor response to increased CSF [Na] is unknown, but it is likely that one or more isoforms that bind ouabain with high affinity are involved (e.g., the Na(+)-K(+)-ATPase alpha(2)- and/or alpha(3)-subunits). We hypothesize that OLS-induced inhibition of the alpha(2)-subunit mediates this response. Therefore, a chronic reduction in alpha(2) expression via a heterozygous gene knockout (alpha(2) +/-) should enhance the pressor response to increased CSF [Na]. Intracerebroventricular (ICV) infusion of artificial CSF containing 0.225 M NaCl increased mean arterial pressure (MAP) in both wild-type (+/+) and alpha(2) +/- mice, but to a greater extent in alpha(2) +/-. Likewise, the pressor response to ICV ouabain was enhanced in alpha(2) +/- mice, demonstrating enhanced sensitivity to brain Na(+)-K(+)-ATPase inhibition per se. The pressor response to ICV ANG I but not ANG II was also enhanced in alpha(2) +/- vs. alpha(2)+/+ mice, suggesting an enhanced brain RAS activity that may be mediated by increased brain angiotensin converting enzyme (ACE). The latter hypothesis is supported by enhanced ACE ligand binding in the organum vasculosum laminae terminalis. These studies demonstrate that chronic downregulation of Na(+)-K(+)-ATPase alpha(2)-isoform expression by heterozygous knockout increases the pressor response to increased CSF [Na] and activates the brain RAS. Since these changes mimic those produced by the endogenous brain OLS, the brain alpha(2)-isoform may be a target for the brain OLS during increases in CSF [Na], such as in salt-dependent hypertension.
Inspirations from Biological Optics for Advanced Photonic Systems (2005)

Observing systems in nature has inspired humans to create technological tools that allow us to better understand and imitate biology. Biomimetics, in particular, owes much of its current development to advances in materials science and creative optical system designs. New investigational tools, such as those for microscopic imaging and chemical analyses, have added to our understanding of biological optics. Biologically inspired optical science has become the emerging topic among researchers and scientists. This is in part due to the availability of polymers with customizable optical properties and the ability to rapidly fabricate complex designs using soft lithography and three-dimensional microscale processing techniques.
Troponin I phosphorylation plays an important role in the relaxant effect of β-adrenergic stimulation in mouse hearts (2004)

Our data support the hypothesis that phosphorylation of cTnI significantly contributes to the enhanced rate of relaxation during beta-adrenergic stimulation.
Side effects of calcium channel blockers. (1988)

Calcium channel blocking drugs are a chemically heterogenous group, so it might be expected that their effects on vascular smooth muscle, cardiac contractility, and conduction tissue may differ. However, the majority of adverse reactions are predictable from their pharmacological actions and may be conveniently grouped in the following categories: 1) vasodilatation, 2) negative inotropic effects, 3) conduction disturbances, 4) gastrointestinal effects, 5) metabolic effects, and 6) drug interactions. Vasodilatory symptoms, namely, dizziness, headaches, flushing sensation, and palpitation, are more likely with nifedipine. Peripheral edema is also common with nifedipine, but the mechanism is uncertain. For a given degree of vasodilation, the greatest negative inotropic effect is seen with verapamil first, diltiazem second, and nifedipine last. Calcium channel blocking drugs are contraindicated in hypertensive patients with second and third degree heart block, sick sinus syndrome, and severe heart failure. Verapamil and diltiazem have a significant effect on cardiac conduction, whereas nifedipine, in therapeutic doses, does not. Local gastrointestinal symptoms, such as nausea and constipation, are common with verapamil. None of the calcium channel blocking drugs have been reported to adversely affect lipid or protein metabolism. However, nifedipine, verapamil, and diltiazem in high doses may inhibit liberation of insulin. The significance of this finding needs to be explored further in hypertensive diabetics. Serum digoxin levels have been shown to increase after administration of verapamil and nifedipine, but there is no evidence that this change has any clinical relevance.(ABSTRACT TRUNCATED AT 250 WORDS)
[A method of iris image quality evaluation]. (2006)

Iris image quality evaluation plays a very important part in iris computer recognition. An iris image quality evaluation method was introduced into this study to distinguish good image from bad image caused by pupil distortion, blurred boundary, two circles appearing not concentric, and severe occlusion by eyelids and eyelashes. The tests based on this method gave good results.
Age-related macular degeneration a new viewpoint (2003)

Age-related macular degeneration (AMD) is a major cause of blindness in the United States. AMD can be categorized into an atrophic (dry) form and a neovascular (wet, exudative) form. The atrophic form involves alterations of pigment distribution, loss of RPE cells and photoreceptors and diminished retinal function due to an overall atrophy of the cells. The neovascular AMD involves proliferation of abnormal choroidal vessels, which penetrate the Bruch's membrane and RPE layer into the subretinal space, thereby forming extensive clots and/or scars. Both environmental and genetic factors are suspected to play a role in AMD. Despite extensive genetic screening of candidate genes only two associations have been identified with AMD (Adenosine triphosphate (ATP)-binding cassette rim (ABCR) protein and apolipoprotein E gene-ApoE). The ABCR protein is retinal specific and accounts for only 3% of AMD cases. ApoE is not specific to the retina, and has been more intriguingly associated with Alzheimer's, another disease of age. The most consistent major risk factor in AMD is age. Our studies on the ACE gene show an association of protection with an Alu element insert, which might be affecting the level of the ACE gene. The ApoE 4 allele and the ACE Alu+/+ genotype have both been shown to be a risk for Alzheimer's and protective for AMD. Given these recent genetic associations, we should examine possible common pathways in diseases of age and their interaction with human genetic polymorphisms.
Combined effect of low-power laser irradiation and anthraquinone anticancer drug aclarubicin on survival of immortalized cells: Comparison with mitoxantrone (2006)

The photodynamic response of the anthraquinone anticancer drug aclarubicin (ACL) was investigated in vitro and compared with that of mitoxantrone (MTX). Cultured immortalized rodent B14 and NIH 3T3 cells were used in the experiments as a model for cells with neoplastic phenotype. Long-term cytotoxicity and inhibition of cell proliferation assayed by the clonal growth and MTT-tetrazolium methods were estimated to compare the efficacy of aclarubicin and mitoxantrone in photosensitizing cells and their death after non-thermal exposure to monochromatic laser light. Green He-Ne (543.5 nm) or red semiconductor (670 nm) low-power laser (LPL) irradiations were applied. Different dose-responses of both cell lines to aclarubicin and mitoxantrone were found so that the cytotoxicity of MTX was considerably greater than the cytotoxicity of ACL. Phototherapy response (P < 0.0001) was observed only for B14 cells after sensitisation with aclarubicin. Under the same conditions no significant effect of red light irradiation (semiconductor 670 nm laser) on survival of both cell lines treated with mitoxantrone was found.
Adaptive Statistical Language Modeling: A Maximum Entropy Approach (2018)

Language modeling is the attempt to characterize, capture and exploit regularities in natural language. In statistical language modeling, large amounts of text are used to automatically determine the model’s parameters. Language modeling is useful in automatic speech recognition, machine translation, and any other application that processes natural language with incomplete knowledge. In this thesis, I view language as an information source which emits a stream of symbols from a ﬁnite alphabet (the vocabulary). The goal of language modeling is then to identify and exploit sources of information in the language stream, so as to minimize its perceived entropy. Most existing statistical language models exploit the immediate past only. To extract information from further back in the document’s history, I use trigger pairs as the basic information bearing elements. This allows the model to adapt its expectations to the topic of discourse. Next, statistical evidence from many sources must be combined. Traditionally, linear interpolation and its variants have been used, but these are shown here to be seriously deﬁcient. Instead, I apply the principle of Maximum Entropy (ME). Each information source gives rise to a set of constraints, to be imposed on the combined estimate. The intersection of these constraints is the set of probability functions which are consistent with all the information sources. The function with the highest entropy within that set is the ME solution. Given consistent statistical evidence, a unique ME solution is guaranteed to exist, and an iterative algorithm exists which is guaranteed to converge to it. The ME framework is extremely general: any phenomenon that can be described in terms of statistics of the text can be readily incorporated. An adaptive language model based on the ME approach was trained on the Wall Street Journal corpus, and showed 32%–39% perplexity reduction over the baseline. When interfaced to SPHINX-II, Carnegie Mellon’s speech recognizer, it reduced its error rate by 10%–14%. The signiﬁcance of this thesis lies in improving language modeling, reducing speech recognition error rate, and in being the ﬁrst large-scale test of the approach. It illustrates the feasibility of incorporating many diverse knowledge sources in a single, uniﬁed statistical framework.
A Neural Network Trained to Identify the Presence of Myocardial Infarction Bases Some Decisions on Clinical Associations That Differ from Accepted Clinical Teaching (1994)

An artificial neural network trained to identify the presence of myocardial infarction has been shown to function with a high degree of accuracy. The effects on network diagnosis of some of the clinical input variables used by this network have previously been shown to be distributed over two distinct maxima. Analysis of the basis for this distribution by studying the specific patterns in which these variables had significantly different impacts on network diagnosis revealed that the differential impacts were due to the contexts in which the variables whose effects were bimodally distributed were placed. These contexts were defined by the values of the other input data used by the network. In a number of instances, the clinical relationships implied by these associations were divergent from prior knowledge about factors predictive of myocardial infarction. One implication of these findings is that this network, which has been shown to perform with a high degree of diagnostic accuracy, may be doing so by identifying relationships between inputted information that are divergent from accepted teaching.
Muscular dystrophy in adult and aged anti-NGF transgenic mice resembles an inclusion body myopathy (2000)

The role of nerve growth factor (NGF) and its receptors in the physiology of skeletal muscles has not been extensively studied in animal models. We describe the production of transgenic lines of mice expressing a neutralizing antibody against NGF (alphaD11) and the morphological and histochemical analysis of skeletal muscles from adult and aged anti-NGF mice. This study reveals that the chronic deprivation of NGF results in a decreased size of myofibers of dorsal and hindlimb muscles in adult but not in postnatal day (P)2 mice. In myofibers from adult anti-NGF mice, the presence of central nuclei, vacuolization of the cytoplasm, and inflammatory cell infiltration was observed. The immunohistochemical analysis of these muscular fibers revealed an upregulation of p75 expression, a decrease in adenosine triphosphatase (ATP)ase activity, and a subsarcolemmal Congo Red-positive staining. Immunostaining with an antibody against amyloid precursor protein showed an increased labeling of the cytoplasm of myofibers from adult and aged anti-NGF mice. These features are reminiscent of human myopathies, such as inclusion body myositis. We conclude that NGF deficits might be relevant for a class of human myopathies.
The influence of anti-zona and anti-sperm antibodies on sperm—egg interactions (1981)

Anti-zona antibodies are effective inhibitors of fertilization in vitro and, regardless of whether passive or active immunization techniques are used, in vivo. Antibodies raised against unfractionated zona pellucida antigens are chiefly directed against a group of carbohydrate-rich components localized on the outer surface of the zona. The interaction of anti-zona antibodies with these sites induces the formation of a surface precipitate which occludes the sperm binding sites by a process of steric hindrance, and stabilizes the zona structure against digestion by the proteolytic enzymes of the sperm head. Active immunization studies indicate that the long-term induction of infertility without adverse side effects is feasible in both laboratory rodents and primates when the zona pellucida is used as a target. Anti-sperm antibodies also exhibit a capacity for inhibiting fertilization in vivo and in vitro. To determine the most appropriate detection method to screen patients for anti-sperm antibodies several homologous and heterologous antisera were analysed by 5 different agglutination and immobilization techniques and then compared for their ability to inhibit the fertilizing capacity of human spermatozoa using the zona-free hamster egg penetration test. The results obtained with the Franklin--Dukes tube--slide test exhibited the closest correlation with the anti-fertility activity of a given antiserum; this activity could be amplified by the addition of complement to the medium. It is concluded that antibodies directed against the sperm head are responsible for limiting the fertilizing capacity of human spermatozoa in vitro and that it is these antibodies of which attention should be focused to unravel the role that immunological factors play in the aetiology of infertility in vivo.