gebhart/patrepeval huggingface dataset:
Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning. (1991)

The complete primary structure of Fel dI (International Union of Immunological Societies nomenclature), the major allergen produced by the domestic cat, Felis domesticus, was determined by protein sequence analysis and cDNA cloning. Protein sequencing of Fel dI from an immunoaffinity-purified extract of house dust revealed that the allergen is composed of two polypeptide chains. Degenerate oligonucleotides derived from the protein sequence were used in polymerase chain reaction amplification of cat salivary gland cDNA to demonstrate that the two chains are encoded by different genes. Chain 1 of Fel dI shares amino acid homology with rabbit uteroglobin, while chain 2 is a glycoprotein with N-linked oligosaccharides.
Transposition of the mariner element from Drosophila mauritiana in zebrafish (1998)

With the increased popularity of zebrafish (Danio rerio) for mutagenesis studies, efficient methods for manipulation of its genome are needed. One approach is the use of a transposable element as a vector for gene transfer in this species. We report here the transformation of zebrafish and germ-line transmission of the mariner element from Drosophila mauritiana. The mariner element was selected because its transposition is independent of host-specific factors. One- to two-cell-stage zebrafish embryos were coinjected with a supercoiled plasmid carrying the nonautonomous mariner element peach and mRNA encoding the transposase. Surviving larvae were reared to adulthood, and the transmission of peach to the F1 generation was tested by PCR. Four of the 12 founders, following plasmid injections on 2 different days, transmitted the element to their progeny. Inheritance of the transgene from the F1 to the F2 generation showed a Mendelian pattern. No plasmid sequences were detected by PCR or Southern blot analysis, indicating transposition of peach rather than random integration of the plasmid DNA. These data provide evidence of transformation of a vertebrate with a transposable element and support the host-independent mechanism for transposition of the mariner element. We suggest this system could be used for insertional mutagenesis or for identifying active regions of the genome in the zebrafish.
Controlled delivery of paclitaxel from stent coatings using novel styrene maleic anhydride copolymer formulations (2008)

The controlled release of paclitaxel (PTx) from stent coatings comprising an elastomeric polymer blended with a styrene maleic anhydride (SMA) copolymer is described. The coated stents were characterized for morphology by scanning electron microscopy (SEM) and atomic force microscopy (AFM), and for drug release using high-performance liquid chromatography (HPLC). Differential scanning calorimetry (DSC) was used to measure the extent of interaction between the PTx and polymers in the formulation. Coronary stents were coated with blends of poly(b-styrene-b-isobutylene-b-styrene) (SIBS) and SMA containing 7% or 14% maleic anhydride (MA) by weight. SEM examination of the stents showed that the coating did not crack or delaminate either before or after stent expansion. Examination of the coating surface via AFM after elution of the drug indicated that PTx resides primarily in the SMA phase and provided information about the mechanism of PTx release. The addition of SMA altered the release profile of PTx from the base elastomer coatings. In addition, the presence of the SMA enabled tunable release of PTx from the elastomeric stent coatings, while preserving mechanical properties. Thermal analysis reveled no shift in the glass transition temperatures for any of the polymers at all drug loadings studied, indicating that the PTx is not miscible with any component of the polymer blend. An in vivo evaluation indicated that biocompatibility and vascular response results for SMA/SIBS-coated stents (without PTx) are similar to results for SIBS-only-coated and bare stainless steel control stents when implanted in the non-injured coronary arteries of common swine for 30 and 90 days.
Low bit rate high quality audio coding with combined harmonic and wavelet representations (2002)

We describe a novel high quality audio coding method using adaptive signal representation, based on sinusoidal and wavelet analysis of signals. First, we perform a harmonic analysis of the signal to remove strong periodic structures or tones from the signal. Then we carry out wavelet analysis that are useful in tracking the transients of the signal. These transients are then removed from the wavelet coefficients. The remaining coefficients have broadband noise-like structure. Since this method separates out tones (sinusoids), transients, and broadband noise, we may use tonal, noise, and temporal masking information to individually encode the tones and the wavelet coefficients. Our experiments suggest that this method yields a nominal bit rate of 1 bit/sample for high quality audio compression.
Explosive breakup of a liquid jet by a swirling coaxial gas jet (1996)

The breakup of a round water jet by a swirling coaxial annular air jet, issuing from convergent jet nozzles has been studied experimentally. The intensity of the swirl and the water to air mass flux ratio have been varied over a large range. It was found that the liquid jet is little affected by the swirl when the swirl number, here defined by the ratio of tangential to axial air jet nozzle velocities, is below a critical value (Scr). Just above this value the liquid jet undergoes an explosive radial expansion and disintegration. A simple model shows that the physically relevant parameters are the gas to liquid momentum flux ratio M and the ratio of the nozzle’s diameters. For small momentum flux ratios, Scr was found to depend on M−1/2 until an asymptotic constant value is reached at large M. Surface tension has no effect on the breakup when air velocities are large, however, membrane-type breakup is dominant at the lower air velocities when the aerodynamic Weber number is of order 100 or less.
Association of Serum Anti-Neuraminidase Antibody with Resistance to Influenza in Man (1972)

The role of serum anti-neuraminidase antibody in resistance to influenzal illness was investigated by administration of wild-type influenza A/Hong Kong/1968 (H3N2) virus to volunteers who possessed varying levels of this antibody but who lacked antibody for the hemagglutinin surface antigen of the virus. Clinical response to the wild-type virus was related to the level of serum anti-neuraminidase antibody. Volunteers in whom influenzal disease with fever developed possessed low levels of serum antibody prior to challenge, whereas men who underwent inapparent infection had a significantly higher mean level of antibody for neuraminidase. Those with afebrile illness had an intermediate level of anti-neuraminidase antibody. Duration of virus excretion and maximal level of virus shed were also inversely related to the titer of serum anti-neuraminidase antibody. These findings provide evidence that antibody directed against influenzal neuraminidase is associated with resistance to clinical expression of influenza A virus in man.
Capacitive Bulk Acoustic Wave Silicon Disk Gyroscopes (2006)

This paper introduces the capacitive bulk acoustic wave (BAW) silicon disk gyroscope. The capacitive BAW disk gyroscopes operate in the frequency range of 2-8MHz, are stationary devices with vibration amplitudes less than 20nm, and achieve very high quality factors (Q) in low vacuum (and even in atmosphere), which simplifies their wafer-scale packaging. The device has lower operating voltages compared to low-frequency gyroscopes, which simplifies the interface circuit design and implementation in standard CMOS
Simple Purification of Highly Active Biotinylated P-Glycoprotein:  Enantiomer-Specific Modulation of Drug-Stimulated ATPase Activity (1999)

A simplified method for the expression and purification of P-glycoprotein (Pgp) is presented. This method is based on the in-frame fusion of both a polyhistidine tail and a 100-amino acid residue biotin acceptor domain of oxaloacetate decarboxylase from Klebsiella pneumoniae at the carboxyl terminus end of Pgp (Pgp-H6BD). The expression/purification protocol for Pgp-H6BD involves high-level expression of the fusion protein in the yeast Pichia pastoris, biotinylation in vitro with biotin ligase, solubilization of crude membrane fractions in detergent, and affinity purification by a combination of nickel and avidin chromatography. Biotinylated Pgp binds to immobilized monomeric avidin and can be eluted with free biotin in a high state of purity. This protocol is rapid and efficient and yields purified Pgp which shows robust ATPase activity, as determined by vanadate-induced trapping of photoactive nucleotides and by direct measurement of ATP hydrolysis by Pgp-H6BD. This method should be useful for structural studies of the protein by spectroscopic or crystallographic approaches. This purified Pgp-H6BD preparation has been used to study the enantiomer-specific effects of inhibitors of Pgp-mediated drug transport on the drug-stimulated ATPase activity of the protein. A series of 1, 4-disubstituted piperazine derivatives with a central chiral carbon and modified at the head and tail groups are shown to stimulate Pgp ATPase activity in a dose-dependent fashion. Some of these compounds are also capable of inhibiting either vinblastine or verapamil stimulation of ATPase activity of Pgp in an enantiomer-specific fashion. The enantiomeric specific inhibitory activity of these compounds suggests complex interactions at a single substrate binding site(s) on Pgp.
Combination Nucleoside/Nucleotide Reverse Transcriptase Inhibitors for Treatment of HIV Infection (2011)

It is likely that N(t)RTIs will continue to dominate the global landscape of HIV treatment and prevention, despite emerging interest in N(t)RTI-free combination therapy. Clinical domains where only few alternatives to N(t)RTIs exist include treatment of HIV/HBV coinfection and HIV-2. There is a need for novel N(t)RTIs with enhanced safety and resistance profiles compared with current N(t)RTIs.
RTP Payload Format for BT.656 Video Encoding (1998)

This document specifies the RTP payload format for encapsulating ITU Recommendation BT.656-3 video streams in the Real-Time Transport Protocol (RTP). Each RTP packet contains all or a portion of one scan line as defined by ITU Recommendation BT.601-5, and includes fragmentation, decoding and positioning information.
Significant elevation of serum levels of eotaxin-3/CCL26, but not of eotaxin-2/CCL24, in patients with atopic dermatitis: serum eotaxin-3/CCL26 levels reflect the disease activity of atopic dermatitis (2003)

Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease characterized by the predominant infiltration of T cells, eosinophils and macrophages in lesional skin. Recently, eotaxin-2/CCL24 and eotaxin-3/CCL26 were identified as CC chemokines that signal exclusively via the CCR3 receptor and have eosinophil-selective chemoattractant activity, as does eotaxin/CCL11. We previously reported that serum levels of thymus and activation-regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 were correlated with the severity of AD. In this report, we investigated the participation of eotaxin-2/CCL24 and eotaxin-3/CCL26 in AD, first measuring the serum levels of eotaxin-2/CCL24 and eotaxin-3/CCL26 in 30 patients with AD, 20 patients with psoriasis vulgaris and 20 healthy controls. The serum levels of eotaxin-3/CCL26 (but not eotaxin-2/CCL24) were significantly higher in patients with AD than in either healthy controls or patients with psoriasis vulgaris; furthermore, the eotaxin-3/CCL26 levels in patients with moderate and severe AD were significantly higher than eotaxin-3/CCL26 levels in patients with mild AD. The serum eotaxin-3/CCL26 levels tended to decrease after treatment, but there was no significant difference between groups. Moreover, the serum eotaxin-3/CCL26 levels were significantly correlated with the serum TARC/CCL17 and MDC/CCL22 levels, eosinophil numbers in peripheral blood and the scoring AD (SCORAD) index. Our study strongly suggests that serum levels of eotaxin-3/CCL26, but not of eotaxin-2/CCL24, have a notable correlation with disease activity of AD and that eotaxin-3/CCL26, as well as TARC/CCL17 and MDC/CCL22, may be involved in the pathogenesis of AD.
Branch Prediction in Multi-Threaded Processors (2000)

There has been a growing interest in the use of multithreading to speed up the execution of a single program. This paper highlights the problems involved in performing accurate branch predictions in single-program multi-threaded (SPMT) processors, where branches are predicted out of program order, and microarchitectural aspects affect branch history. In particular, it shows that the recorded branch history could become insufficient, discontinuous, outdated, scrambled, or inaccurate, depending on the specifics of the microarchitecture. Measurements obtained with a multiscalar simulator show that the affects on the recorded branch history cause the branch misprediction ratios to increase from an average of 6.5% to an average of 41.0% for global branch history-based schemes such as gshare, and from an average of 6.7% to an average of 11.5% for per-address history-based schemes such as Fag. The paper also investigates techniques for overcoming the adverse effects of the microarchitecture's impact on branch history. These techniques rely on extrapolation of outdated history so as to make it up-to-date, and correlation with thread-level information so as to partition scrambled history into a set of unscrambled history parts. Experimental evaluation of the proposed techniques on a multiscalar simulator indicates that these techniques, especially a hybrid of extrapolation and correlation, can substantially lower the branch misprediction ratios.
Quantitation of Bacteria in Blood of Typhoid Fever Patients and Relationship between Counts and Clinical Features, Transmissibility, and Antibiotic Resistance (1998)

Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood bacterial counts were estimated by the pour plate method. The median S. typhi count in blood was 1 CFU/ml (range, <0.3 to 387 CFU/ml), of which a mean of 63% (95% CI, 58 to 67%) were intracellular. The mean number of bacteria per infected leukocyte was 1.3 (interquartile range [IQR], 0.7 to 2.4) CFU/cell (n = 81). Children (< 15 years old; n = 115) had higher median blood bacterial counts than adults (n = 262): 1.5 (range, <0.3 to 387) versus 0.6 (range, <0.3 to 17.7) CFU/ml (P = 0.008), and patients who excreted S. typhi in feces had higher bacteremias than those who did not: a median of 3 (range, <0.3 to 32) versus 1 (range, <0.3 to 68) CFU/ml (P = 0.02). Blood bacterial counts declined with increasing duration of illness (P = 0.002) and were higher in infections caused by multidrug-resistant S. typhi (1.3 [range, <0.3 to 387] CFU/ml; n = 313) than in infections caused by antibiotic-sensitive S. typhi (0.5 [range, <0.3 to 32] CFU/ml; n = 62) (P = 0.006). In a multivariate analysis this proved to be an independent association, suggesting a relationship between antibiotic resistance and virulence in S. typhi.
GsdmD p30 elicited by caspase-11 during pyroptosis forms pores in membranes (2016)

Gasdermin-D (GsdmD) is a critical mediator of innate immune defense because its cleavage by the inflammatory caspases 1, 4, 5, and 11 yields an N-terminal p30 fragment that induces pyroptosis, a death program important for the elimination of intracellular bacteria. Precisely how GsdmD p30 triggers pyroptosis has not been established. Here we show that human GsdmD p30 forms functional pores within membranes. When liberated from the corresponding C-terminal GsdmD p20 fragment in the presence of liposomes, GsdmD p30 localized to the lipid bilayer, whereas p20 remained in the aqueous environment. Within liposomes, p30 existed as higher-order oligomers and formed ring-like structures that were visualized by negative stain electron microscopy. These structures appeared within minutes of GsdmD cleavage and released Ca(2+) from preloaded liposomes. Consistent with GsdmD p30 favoring association with membranes, p30 was only detected in the membrane-containing fraction of immortalized macrophages after caspase-11 activation by lipopolysaccharide. We found that the mouse I105N/human I104N mutation, which has been shown to prevent macrophage pyroptosis, attenuated both cell killing by p30 in a 293T transient overexpression system and membrane permeabilization in vitro, suggesting that the mutants are actually hypomorphs, but must be above certain concentration to exhibit activity. Collectively, our data suggest that GsdmD p30 kills cells by forming pores that compromise the integrity of the cell membrane.
Heterocyclic Amidines: I. A One-Step Synthesis of New a-Substituted Imidazolylphenylacetic Acids (1995)

Absh.act -The reaction of a-phenyl-R-formylacrylic derivatives with various amidines (benzamidine, acetamidine) and heterocyclic amidines (2-aminopyridines, 3-aminopyridazines), yields asubstituted imidazolylphenylacetic acids and imidazo[l,2-xlazines bearing a phenylacetic acid residue in position 3.Some mechanistic aspects of the cyclocondensation reaction will be discussed.Within the scope of our current research on original benzodiazepine receptor ligands,l-3 we considered of special interest the synthesis of a series of 34a-phenyl ethoxycarbonylmethy1)-
Effects of interleukin‐6 on the metabolism of connective tissue components in rheumatoid synovial fibroblasts (1992)

These results suggest that in RA, IL-6 may participate along with IL-1 in fine tuning of the catabolism of connective tissue components, by modulating the balance between connective tissue-degrading enzymes and their inhibitors.
Establishment and Characterization of Monoclonal and Polyclonal Antibodies Against Human Intestinal Fatty Acid‐Binding Protein (I‐FABP) using Synthetic Regional Peptides and Recombinant I‐FABP (2007)

We have succeeded in raising highly specific anti-human intestinal fatty acid-binding protein (I-FABP) monoclonal antibodies by immunizing animals with three synthetic regional peptides, i.e., the amino terminal (RP-1: N-acetylated 1-19-cysteine), middle portion (RP-2: cysteinyl-91-107) and carboxylic terminal (RP-3: cysteinyl-121-131) regions of human I-FABP, and the whole I-FABP molecule as antigens. We also raised a polyclonal antibody by immunizing with a recombinant (r) I-FABP. To ascertain the specificity of these antibodies for human I-FABP, the immunological reactivity of each was examined by a binding assay using rI-FABP, partially purified native I-FABP and related proteins such as liver-type (L)-FABP, heart-type (H)-FABP, as well as the regional peptides as reactants, and by Western blot analysis. In addition, the expression and distribution of I-FABP in the human gastrointestinal tract were investigated by an immunohistochemical technique using a carboxylic terminal region-specific monoclonal antibody, 8F9, and a polyclonal antibody, DN-R2. Our results indicated that both the monoclonal and polyclonal antibodies established in this study were highly specific for I-FABP, but not for L-FABP and H-FABP. Especially, the monoclonal antibodies raised against the regional peptides, showed regional specificity for the I-FABP molecule. Immunoreactivity of I-FABP was demonstrated in the mucosal epithelium of the jejunum and ileum by immunohistochemical staining, and the immunoreactivity was based on the presence of the whole I-FABP molecule but not the presence of any precursors or degradation products containing a carboxylic terminal fragment. It is concluded that some of these monoclonal and polyclonal antibodies, such as 8F9, 4205, and DN-R2, will be suitable for use in research on the immunochemistry and clinical chemistry of I-FABP because those antibodies can recognize both types of native and denatured I-FABP. In order to detect I-FABP in blood samples, it is essential to use this type of antibody, reactive to native type of I-FABP. It is anticipated that, in the near future, such a method for measuring I-FABP will be developed as a useful tool for diagnosing intestinal ischemia by using some of these antibodies.
DNA Nucleoside Interaction and Identification with Carbon Nanotubes (2006)

We investigate the interaction of individual DNA nucleosides with a carbon nanotube (CNT) in vacuum and in the presence of external gate voltage. We propose a scheme to discriminate between nucleosides on CNTs based on measurement of electronic features through a local probe such as scanning tunneling spectroscopy. We demonstrate through quantum mechanical calculations that these measurements can achieve 100% efficiency in identifying DNA bases. Our results support the practicality of ultrafast DNA sequencing using electrical measurements.
The effect of restricting energy intake on diabetes in Psammomys obesus. (1994)

The aim of this paper was to determine whether restricting energy intake would reduce the elevated levels of glucose, insulin, cholesterol and triglyceride in diabetic Psammomys obesus (sand rat). Between 11 and 12 weeks of age Psammomys obesus were divided into three groups based on blood glucose and plasma insulin levels in the fed ad libitum state; group 1 was normoglycemic (4.4 +/- 0.3 mM) and normoinsulinemic (0.46 +/- 0.04 ng/ml), group 2 was normoglycemic (5.0 +/- 0.3 mM) and hyperinsulinemic (3.58 +/- 0.62 ng/ml) and group 3 was hyperglycemic (11.2 +/- 1.2 mM) and hyperinsulinemic (6.23 +/- 0.73 ng/ml). Energy intake was restricted to 67% of normal for 2 weeks before ad libitum feeding was resumed for a further 2 weeks. Animals in group 3 developed the most abnormalities when compared to group 1 including increased levels of food intake (16.3 +/- 0.5 vs 14.2 +/- 0.5 g/day, P < 0.05), body weight (192 +/- 5 vs 162 +/- 4 g, P < 0.05), triglycerides (1.5 +/- 0.2 vs 0.96 +/- 0.08 mM, P < 0.05), and cholesterol (2.8 +/- 0.2 vs 2.1 +/- 0.1 mM, P < 0.05). In group 3, food restriction was effective in reducing glucose levels (but not insulin) both during and following the restriction period respectively (11.2 +/- 1.2 vs 4.6 +/- 0.5, and 5.9 +/- 1.3, mM, P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Antagonistic Activities of Bacillus spp. Strains Isolated from Tidal Flat Sediment Towards Anthracnose Pathogens Colletotrichum acutatum and C. gloeosporioides in South Korea (2015)

Anthracnose is a fungal disease caused by Colletotrichum species that is detrimental to numerous plant species. Anthracnose control with fungicides has both human health and environmental safety implications. Despite increasing public concerns, fungicide use will continue in the absence of viable alternatives. There have been relatively less efforts to search antagonistic bacteria from mudflats harboring microbial diversity. A total of 420 bacterial strains were isolated from mudflats near the western sea of South Korea. Five bacterial strains, LB01, LB14, HM03, HM17, and LB15, were characterized as having antifungal properties in the presence of C. acutatum and C. gloeosporioides. The three Bacillus atrophaeus strains, LB14, HM03, and HM17, produced large quantities of chitinase and protease enzymes, whereas the B. amyloliquefaciens strain LB01 produced protease and cellulase enzymes. Two important antagonistic traits, siderophore production and solubilization of insoluble phosphate, were observed in the three B. atrophaeus strains. Analyses of disease suppression revealed that LB14 was most effective for suppressing the incidence of anthracnose symptoms on pepper fruits. LB14 produced antagonistic compounds and suppressed conidial germination of C. acutatum and C. gloeosporioides. The results from the present study will provide a basis for developing a reliable alternative to fungicides for anthracnose control.