gebhart/patrepeval huggingface dataset:
The Pleotrophic Effects of Insulin-Like Growth Factor-I on Human Spinal Cord Neural Progenitor Cells (2010)

Most stem cell therapies involve direct, intraparachymal placement of neural progenitor cells. These cells provide physical support to the endogenous neuronal population and may be engineered to provide in situ growth factor support. Insulin-like growth factor-I (IGF-I) has potent neurotrophic and neuroprotective properties and is expressed by human neural stem cells (hNSCs). IGF-I is implicated in multiple aspects of cell behavior, including proliferation, differentiation, and survival. Enhancing hNSC function through IGF-I overexpression may increase the benefits of stem cell therapy. As a first step to that goal, we examined the direct effects of IGF-I on hNSC behavior in vitro. We demonstrate that IGF-I treatment enhances both the number and length of hNSC neurites. This is correlated with a decrease in proliferation, suggesting that IGF-I promotes neurite outgrowth but not proliferation. While IGF-I activates both AKT and MAPK signaling in hNSCs, we demonstrate that IGF-I-mediated neurite outgrowth is dependent only on AKT signaling. Finally, we demonstrate that IGF-I is neuroprotective after glutamate exposure in a model of excitotoxic cell death.
Thrombin immobilized to extracellular matrix is a potent mitogen for vascular smooth muscle cells: nonenzymatic mode of action. (1990)

Esterolytically inactive diisopropyl fluorophosphate-conjugated thrombin (DIP-alpha-thrombin) stimulated 3H-thymidine incorporation and proliferation of growth-arrested vascular smooth muscle cells (SMCs), similar to native alpha-thrombin. Half-maximal mitogenic response of SMCs was obtained at 1 nM thrombin and was specifically blocked by the leech-derived, high-affinity thrombin inhibitor, hirudin. Native thrombin and a variety of thrombin species that were chemically modified to alter thrombin procoagulant or esterolytic functions were found to induce 3H-thymidine incorporation to a similar extent. Exposure of SMCs to DIP-alpha-thrombin caused a rapid and transient expression of the c-fos protooncogene, determined by Northern blot analysis. These results indicate that thrombin is a potent mitogen for SMCs through a distinct non-enzymatic domain. Binding of 125I-alpha-thrombin to SMC cultures revealed an apparent dissociation constant of 6 nM and an estimated 5.4 x 10(5) binding sites per cell. This binding was inhibited to the same extent by native thrombin and by its nonenzymatic form, DIP-alpha-thrombin. Moreover, the chemotactic fragment of thrombin (CB67-129), which failed to elicit a mitogenic response, competed for 125I-alpha-thrombin binding to SMCs. Cross-linking analysis of 125I-alpha-thrombin to SMCs revealed a specific cell-surface binding site 55 kDa in size. Finally, thrombin immobilized to a naturally produced extracellular matrix retained potent mitogenic activity toward SMCs. These observations lend support to the possibility that in vivo, subendothelial basement membranes sequester thrombin (as well as other bioactive molecules), which may stimulate localized and persistent growth of arterial SMCs. Thrombin may thus be involved directly in progression of atherosclerotic plaque formation.
Evaluation of epidermal growth factor receptor, carcinoembryonic antigen and Lewis carbohydrate antigens in human colorectal and liver neoplasias (2001)

The expression of LewisY related carbohydrate antigens and the content of epidermal growth factor receptor (EGF-R), the carcinoembryonic antigen (CEA) and the alpha-fetoprotein (AFP) in colorectal and liver tumors were determined. These included 30 large bowel adenocarcinomas (7 colon, 6 sigma, 5 caecum, 12 rectum), 12 hepatocellular carcinomas and 6 liver metastases. Histologically normal tissue excised along with the tumors were used as controls. All plasma membranes studied showed specific EGF binding, and tumor plasma membranes had an EGF receptor level higher than that of the normal counterpart. However, EGF-R was positive in only a few tumors, and no correlation between clinical stages and grades of differentiation was observed. Cytosol CEA was higher in tumors than in normal counterparts. Tissue AFP and CEA content was different in liver hepatocellular carcinomas and in liver metastases. They are good markers to differentiate between primary and secondary liver neoplasias. The LewisY and related carbohydrate antigens, evaluated by the reactivity of the tissues to monoclonal antibody MAb B3, are expressed in liver metastases from colorectal adenocarcinoma. MAb B3 failed to react with hepatocellular carcinomas and with peritumoral liver tissues obtained from both metastatic and primary tumor lesions. These data suggest that immunoblotting with MAb B3 may be useful to obtain more information on liver carcinomas. Furthermore, MAb B3 or CEA armed with toxin in the form of recombinant immunotoxin or linked to a radionuclide can be useful in new treatments of metastatic lesions, such as immunotherapy, radioimmunotherapy and radioimmunoguided surgery.
Wellbore Stability of Extended Reach Wells in an Oil Field in Sarawak Basin, South China Sea (2004)

Abstract The development plan of an oil field in the Sarawak Basin, South China Sea includes extended reach wells. Drilling experience in exploration wells highlighted the issue of wellbore instability in the region. A wide range of wellbore instability-related problems has been experienced, ranging from tight hole (remedied by reaming) to overpull and stuck tool, and a range of drilling fluid designs has been implemented. There was considerable concern regarding wellbore stability, including coal bed instability, its effect on drilling performance and the potential for this to severely impact both schedule and budget. Subsequently, a wellbore stability study, with an emphasis on extended reach wells planned for the field development, was conducted. The aim of the study was to develop a strategy to maintain mechanical and time-dependent stability of the extended reach wells to ensure the well construction encounters minimum wellbore instability-related problems. With the availability of additional well data and the need to push the technical limit of the drilling campaign, a follow-up review study was conducted and the additional information was used to refine the critical mud weight contour plots for a range of wellbore enlargements. This paper describes the outline of the study, the approach adopted in the development of the strategies and the utilisation of the results from the two studies to develop recommendations for the mud weight programs. The drilling experience, material properties, typical in-situ stresses and formation pressure in the field are presented and discussed. Based on the gathered drilling experience, composite logs and laboratory tests conducted on downhole core materials, dominant failure mechanisms for the key formations and coal beds were identified. An empirical approach was adopted for the prediction of lower bound mud weights for wellbore enlargements ranging form 10% to 40% of wellbore diameter. Examples of critical mud weight contour plots and pore pressure change tables which form part of the strategy for designing optimal drilling fluid program are presented.
Discovery and Mechanism of Highly Efficient Cyclic Cell-Penetrating Peptides (2016)

Previous cell-penetrating peptides (CPPs) generally have low cytosolic delivery efficiencies, because of inefficient endosomal escape. In this study, a family of small, amphipathic cyclic peptides was found to be highly efficient CPPs, with cytosolic delivery efficiencies of up to 120% (compared to 2.0% for Tat). These cyclic CPPs bind directly to the plasma membrane phospholipids and enter mammalian cells via endocytosis, followed by efficient release from the endosome. Their total cellular uptake efficiency correlates positively with the binding affinity for the plasma membrane, whereas their endosomal escape efficiency increases with the endosomal membrane-binding affinity. The cyclic CPPs induce membrane curvature on giant unilamellar vesicles and budding of small vesicles, which subsequently collapse into amorphous lipid/peptide aggregates. These data suggest that cyclic CPPs exit the endosome by binding to the endosomal membrane and inducing CPP-enriched lipid domains to bud off as small vesicles. Together with their high proteolytic stability, low cytotoxicity, and oral bioavailability, these cyclic CPPs should provide a powerful system for intracellular delivery of therapeutic agents and chemical probes.
Transparent, Live Migration of a Software-Defined Network (2014)

Increasingly, datacenters are virtualized and software-defined. Live virtual machine (VM) migration is becoming an indispensable management tool in such environments. However, VMs often have a tight coupling with the underlying network. Hence, cloud providers are beginning to offer tenants more control over their virtual networks. Seamless migration of all (or part) of a virtual network greatly simplifies management tasks like planned maintenance, optimizing resource usage, and cloud bursting. Our LIME architecture efficiently migrates an ensemble, a collection of virtual machines and virtual switches, for any arbitrary controller and end-host applications. To minimize performance disruptions, during the migration, LIME temporarily runs all or part of a virtual switch on multiple physical switches. Running a virtual switch on multiple physical switches must be done carefully to avoid compromising application correctness. To that end, LIME merges events, combines traffic statistics, and preserves consistency among multiple physical switches even across changes to the packet-handling rules. Using a formal model, we prove that migration under LIME is transparent to applications, i.e., any execution of the controller and end-host applications during migration is a completely valid execution that could have taken place in a migration-free setting. Experiments with our prototype, built on the Floodlight controller, show that ensemble migration can be an efficient tool for network management.
Characterization of the Antigen‐Specific T Cell Receptor from a Pigeon Cytochrome c‐Specific T Cell Hybrid (1984)

The monoclonal antibody, A2B4-2, specifically bound to and inhibited antigen-induced IL-2 release from the cloned pigeon cytochrome c-specific T cell hybrid, 2B4. The initial immunoprecipitation with these reagents demonstrated a protein of 85-90kd on non-reducing conditions, and two bands of 45-50 and 40-44 on reducing conditions. These data enabled us to conclude that this monoclonal antibody bound to the antigen receptor on the 2B4 cell. In this paper we have presented results that further define the receptor structure. The migration pattern of the protein on SDS-PAGE in the presence and absence of reducing agents was consistent with the interpretation that the receptor has intrachain disulfide bonds that create globular domains. Sequence data from the recently described cDNA clones that encode receptor genes confirm that there are cysteine residues in positions that could be involved in such disulfide bonding. Since both alpha and beta chains behave identically on the SDS-PAGE we predict that both chains have the same double domain structure. The antigen receptor has a heterodimeric structure. A relatively simple acidic alpha chain is bound to one of two forms of the beta chain. Some of the receptors have a beta chain equal in molecular weight to the alpha chain, while some of the beta chains (beta') are heavier and more acidic. The difference in beta chain forms appears to be due to different levels of glycosylation of this chain. The cDNA sequence data demonstrate that there are several possible carbohydrate addition sites on the protein encoded by this gene so it may be that 2B4 beta chains are present that are either completely or partially glycosylated at these sites. Finally, we have presented a preliminary experiment that indicates that a smaller 20-25kd molecule is associated with the antigen receptor. The well characterized T3 molecule appears to be in a complex with the clonotypic structure on human T cells. The crosslinking data that we present suggests that a similar molecule may be present in the murine system.
Temperatur-Messungen in turbulenten Drallflammen: Thermoelemente im Vergleich zu Laser-Raman-Streuung (2004)

In a gas turbine-like methane/air flame with a thermal load of 35 kW, temperature measurements have been performed using a simple thermocouple and laser Raman scattering. The results obtained with the different techniques have been compared. The goals of the study were the quantification of the differences and the clarification of the observed deviations of the thermocouple measurements. For the interpretation of the deviations, the results from additional measurements of the velocity field and gas composition in the flame were of particular importance. Temperature differences T Raman - T Thermoel between -250 K and 400 K were observed which are mainly due to the characteristics of the thermocouple. In particular, the paper addresses the influence of thermal radiation of the thermocouple, the disturbance of the flow field by the physical probe, and temporal averaging effects. The results are displayed as two-dimensional distributions of temperature, temperature differences, reaction progress, and flow velocities, as well as of the fluctuations of the temperature and velocities.
A phase II study of irinotecan plus cisplatin for patients with advanced stage IIIB or IV NSCLC previously treated with nonplatinum‐based chemotherapy (2006)

The irinotecan and cisplatin combination is an active and well-tolerated regimen for the patients with advanced NSCLC that progressed after nonplatinum-containing regimen(s).
Increased antiplatelet T helper lymphocyte reactivity in patients with autoimmune thrombocytopenia (1991)

Chronic autoimmune thrombocytopenic purpura (ATP) is a common hematologic disorder in which platelet-specific autoantibodies bind to platelets and enhance their destruction by the reticuloendothelial system. While there has been considerable investigation of the humoral immune abnormalities in ATP, little work has been performed on the cellular immunoregulatory aspects of this autoimmune disorder. We describe here that patients with ATP have lymphocytes that proliferate normally when stimulated by mitogens. However, when stimulated by normal control platelets in 7-day antigen-presenting cell cultures, peripheral blood mononuclear cells (PBMC) from patients with ATP proliferate at significantly higher levels (P less than .001) and their lymphocytes secrete significantly higher amounts of interleukin-2 (IL-2) (P less than .001) than do lymphocytes from control subjects. Depletion studies with monoclonal anti-CD8 and complement did not reduce the proliferative capacity of the responding PBMC population, indicating that CD4+ T-helper cells may be responsible for the response. Phenotypic analysis of peripheral blood lymphocyte subsets from patients with ATP showed that there was a significant reduction in CD4+Leu8+ T suppressor-inducer cells (P less than .001) and a concomitant increase in CD3+DR+ activated T cells (P less than .001) and CD19+ B cells (P less than .05). These data indicate that CD4+ T-helper cells from patients with ATP are stimulated by normal platelet antigen(s) to secrete IL-2 and may modulate the enhanced antiplatelet autoantibody response.
Graphene quantum point contact transistor for DNA sensing (2013)

By using the nonequilibrium Green's function technique, we show that the shape of the edge, the carrier concentration, and the position and size of a nanopore in graphene nanoribbons can strongly affect its electronic conductance as well as its sensitivity to external charges. This technique, combined with a self-consistent Poisson-Boltzmann formalism to account for ion charge screening in solution, is able to detect the rotational and positional conformation of a DNA strand inside the nanopore. In particular, we show that a graphene membrane with quantum point contact geometry exhibits greater electrical sensitivity than a uniform armchair geometry provided that the carrier concentration is tuned to enhance charge detection. We propose a membrane design that contains an electrical gate in a configuration similar to a field-effect transistor for a graphene-based DNA sensing device.
Inhibition of Silencing and Accelerated Aging by Nicotinamide, a Putative Negative Regulator of Yeast Sir2 and Human SIRT1 (2002)

The Saccharomyces cerevisiae Sir2 protein is an NAD(+)-dependent histone deacetylase that plays a critical role in transcriptional silencing, genome stability, and longevity. A human homologue of Sir2, SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis. The Sir2 deacetylation reaction generates two products: O-acetyl-ADP-ribose and nicotinamide, a precursor of nicotinic acid and a form of niacin/vitamin B(3). We show here that nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity. We show that physiological concentrations of nicotinamide noncompetitively inhibit both Sir2 and SIRT1 in vitro. The degree of inhibition by nicotinamide (IC(50) < 50 microm) is equal to or better than the most effective known synthetic inhibitors of this class of proteins. We propose a model whereby nicotinamide inhibits deacetylation by binding to a conserved pocket adjacent to NAD(+), thereby blocking NAD(+) hydrolysis. We discuss the possibility that nicotinamide is a physiologically relevant regulator of Sir2 enzymes.
Privacy-Preserving Support Vector Machine Training Over Blockchain-Based Encrypted IoT Data in Smart Cities (2019)

Machine learning (ML) techniques have been widely used in many smart city sectors, where a huge amount of data is gathered from various (IoT) devices. As a typical ML model, support vector machine (SVM) enables efficient data classification and thereby finds its applications in real-world scenarios, such as disease diagnosis and anomaly detection. Training an SVM classifier usually requires a collection of labeled IoT data from multiple entities, raising great concerns about data privacy. Most of the existing solutions rely on an implicit assumption that the training data can be reliably collected from multiple data providers, which is often not the case in reality. To bridge the gap between ideal assumptions and realistic constraints, in this paper, we propose secureSVM, which is a privacy-preserving SVM training scheme over blockchain-based encrypted IoT data. We utilize the blockchain techniques to build a secure and reliable data sharing platform among multiple data providers, where IoT data is encrypted and then recorded on a distributed ledger. We design secure building blocks, such as secure polynomial multiplication and secure comparison, by employing a homomorphic cryptosystem, Paillier, and construct a secure SVM training algorithm, which requires only two interactions in a single iteration, with no need for a trusted third-party. Rigorous security analysis prove that the proposed scheme ensures the confidentiality of the sensitive data for each data provider as well as the SVM model parameters for data analysts. Extensive experiments demonstrates the efficiency of the proposed scheme.
Surgery for Ménière's disease (2013)

The two trials included in this review provide insufficient evidence of the beneficial effect of endolymphatic sac surgery in Ménière's disease.
Marked differences in the antigenic structure of human respiratory syncytial virus F and G glycoproteins (1989)

Monoclonal antibodies directed against the glycoproteins of human respiratory syncytial virus were used in competitive enzyme-linked immunosorbent assays for topological mapping of epitopes. Whereas epitopes of the F glycoprotein could be ascribed to five nonoverlapping antigenic sites, anti-G antibodies recognized unique epitopes, many of whose competition profiles overlapped extensively. Variant viruses selected with a neutralizing (47F) anti-F antibody lost the binding for only 47F and 49F antibodies, which mapped in the same antigenic area. In contrast, viruses selected with an anti-G antibody lost the capacity to bind most of the anti-G antibodies, and their G protein was not recognized by an anti-virus antiserum, indicating major changes in the antigenic structure of the G molecule. Finally, we found great antigenic variation of the G protein among viral isolates. This occurred even within viruses of the same subtype with only limited divergence of amino acid sequence between strains. All of these data indicate marked differences in the antigenic organization of the G and F glycoproteins of respiratory syncytial virus; we discuss these differences in terms of the chemical structure of the glycoproteins.
Biquadratic transconductance switched capacitor filters (2003)

The author presents a new type of sampled data filters consisting of transconductance, switches, and capacitors, called transconductor switched capacitor (TSC) filters. The implementation of this type of filter is particularly suitable for the CMOS integrated circuit technologies. The synthesis of biquadratic TSC filters is explained based on a signal flow graph realizing the general z-domain second-order transfer function. The filter coefficients are determined by the clock pulse width and the transconductances, both of which can be turned, and the integrator capacitors. By an appropriate choice of the clock pulse width the silicon area consumed for the transconductors and the capacitors can be optimized.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Effects of Li Content on Structure and Electrochemical Properties of Li[sub 1+x](Ni[sub 0.5]Mn[sub 0.5])[sub 1−x]O[sub 2] (0≤x≤0.15) Electrodes in Lithium Cells (1.0–4.8 V) (2007)

Layered materials have been synthesized using and coprecipitated . The materials with increased lithium content showed distinct differences in particle morphology, which is attributed to the melting of excess during calcination. The powder XRD patterns and the initial charging curves to indicated that the excess lithium resides in the transition metal layer to form -like domains in the electrode structure such that these compounds can be reformulated in a two-component composite notation as with some cation disorder between the two components. The excess lithium significantly affected the charge/discharge behaviors in the low-voltage range and the rate capability of the electrode materials, which is attributed to increasing content and decreasing disorder (i.e., Ni content in the lithium layer) in the materials.