gebhart/patrepeval huggingface dataset:
Deficiency of N-glycolylneuraminic acid and Galα1-3Galβ1-4GlcNAc epitopes in xenogeneic cells attenuates cytotoxicity of human natural antibodies (2010)

A DKO mice strain lacking both Gal and NeuGc antigens in several tissues has been generated. Comparing CDC of human sera against mouse thymocytes, the DKO strain shows a statistically significant but a small additional reduction in CDC compared to that already achieved in GalT(-/-) mice.
A simplified synthesis for meso-tetraphenylporphine (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA simplified synthesis for meso-tetraphenylporphineAlan D. Adler, Frederick R. Longo, John D. Finarelli, Joel Goldmacher, Jacques Assour, and Leonard KorsakoffCite this: J. Org. Chem. 1967, 32, 2, 476Publication Date (Print):February 1, 1967Publication History Published online1 May 2002Published inissue 1 February 1967https://pubs.acs.org/doi/10.1021/jo01288a053https://doi.org/10.1021/jo01288a053research-articleACS PublicationsRequest reuse permissionsArticle Views26228Altmetric-Citations2163LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Skeletal Muscle Protein Synthesis and Growth Hormone Secretion in Young Lambs Treated with Clenbuterol (1989)

To determine effects of clenbuterol (CB) on muscle protein turnover and growth hormone (GH) secretion, 16 crossbred wether lambs (14.4 kg) were randomized into two groups designated to receive daily oral boluses of gelatin capsules containing corn starch with either 0 (control, CTL) or 1.87 mg/kg body weight CB for either 14 (n = 8) or 28 d (n = 8). This calculates to be approximately 40 mg CB/kg diet. Lambs had ad libitum access to a 16% crude protein corn-soy diet and feed consumption (FC) was measured. After 14 and 28 d, lambs were slaughtered and semitendinosus (ST), longissimus (LD) and brachialis (BR) muscles were exercised, weighed and analyzed for protein (TP) content. For 6 h prior to slaughter of 28-d lambs, 2.5 microCi L-[U-14C]tyrosine/kg was infused intravenously, blood was sampled and plasma was analyzed for specific radioactivity of tyrosine. Plasma GH concentrations were assessed by radioimmunoassay. No differences due to treatment were found in FC, rate of gain or GH concentrations. Semitendinosus and BR weights of control lambs at 14 d did not differ between treatments. At 28 d, ST and BR weights of control lambs (58.8 and 18.5 g, respectively) were less (P less than .10) than those of lambs treated with CB (74.3 and 23.1 g, respectively). The TP per ST and BR at 28 d for control lambs was 71.5 and 85.1% (P less than .10) that of muscles of lambs treated with CB. Fractional protein synthesis rates (FSR) of the BR (9.4 vs 6.1%/d) and total protein synthesized in ST muscle per day (1.4 vs .8 g) were elevated (P less than .10) in lambs treated with CB compared to controls. These data suggest that the increased fractional accretion rate observed in lambs treated with CB for 28 d was caused by increased FSR.
MEASURING STAFF MEMBERS E READINESS TOWARDS E LEARNING AT EGYPTIAN FACULTIES OF TOURISM AND HOTELS (2010)

Information and Communication Technology (ICT) has made life much different than it was before especially in Education. E learning is becoming increasingly prominent in higher education, with universities increasing provision and more students signing up. E learning in the university context is influenced by a number of factors. However, the researcher particular interest in this paper in the e readiness of the staff memberfor e learning at the Egyptian faculties of tourism and hotels in Fayoum, Menia, Helwan and Alex. This is to the increasing and flexible market that is difficult to research by only traditional education.This research measures the staff members’ e readiness for e learning at the faculties of tourism and hotels in Egypt which influenced by a number of factors and dimensions. These are technical and pedagogical competences, experience scale and attitude Scale but the research will concentrate on the first dimension. This may help Tourism faculties to promote the use of IT in teaching and learning and also apply e learning effectively in these faculties to make qualified students for market work. Data was collected through a questionnaire of 92 staff member (professor, assistant professor and lecturers) of tourism studies, hotel management and Tourism Guidance departments. Also this research is based on a basic hypothesis that there is a shortage and insufficient of staff members e readiness for e learning. The paper contains also typical e learning quality framework , SPSS program was used to analyses the data and reach to the finding of this study as frequencies, standard deviation, means, t test per pair between the two dimension pedagogical and technical competencies, also average mean to measure this dimension, also cronbach alpha was made to ensure the reliability, beside the validity was been achieved. The findings have been indicated that the staff member at faculties of tourism and hotels have a good level in pedagogical factor and have a bad or shortage in technical factor, so the paper has recommended them to improve their technical competencies.
CO2 laser welding of deep drawing steel sheet and microalloyed steel plate (1983)

Laser welding trials have been conducted on 2 to 4mm (0.080 to 0.160 inch) low carbon deep drawing steel in both butt and T joint configurations, and on butt joints in 12.5mm (0.490 inch) microalloyed steel. A 5kW fast axial flow CO2 laser used. The incidence of defects and the weld structures and properties are described.
Phase Homogeneity in Y 2 O 3 – MgO Nanocomposites Synthesized by Thermal Decomposition of Nitrate Precursors with Ammonium Acetate Additions (2011)

Nanocomposite powders with equal volume fractions of Y 2 O 3 and MgO have been produced by the thermal decomposition of precursor mixtures of yttrium nitrate and magnesium nitrate. Solutions of the precursor salts were mixed with ammonium acetate fuel, dried to form a gel‐like substance, and then calcined to give nanocrystalline ceramic powders. The amount of ammonium acetate added to the metal nitrate precursors was varied systematically, and the morphology and distribution of the component phases in consolidated compacts of the resultant ceramic powders were examined by a combination of focused ion beam sectioning, scanning, and transmission electron microscopy. The dispersion of the Y 2 O 3 and MgO phases within the synthesized powders improved, and the sizes of the phase domains reduced, with increasing ammonium acetate content up to the quantity required for a stoichiometric redox reaction with the metal nitrates. The addition of excess ammonium acetate gave no further improvement in phase domain dispersion or reduction in phase domain sizes. These phenomena are related to the thermal characteristics for the decomposition of the precursors and their effect on phase separation during oxide crystallization.
Electrochemical reduction of pyridopyrazines (1978)

The electrochemical reduction of several pyrido[2,3-b]pyrazines in hydroorganic or aprotic media leads to 1,4-dihydro derivatives which can be isolated in some cases. Most of these derivatives isomerize into 1,2-, 3,4-, or 5,8-dihydro compounds. A molecule of alcohol is added to these last compounds in alkaline medium. The electrochemical reduction of 1,2- or 3,4-dihydro derivatives gives 1,2,3,4-tetrahydropyridopyrazines. In hydroorganic media, the 7-bromopyrido [2,3-b]pyrazines give a 1,4-dihydro compound which can either isomerize or debromhydrate. In an aprotic medium, the parent heterocycle is obtained. By electrochemical reduction of pyrido[3,4-b]pyrazines, 1,4-dihydro derivatives can be formed which isomerize. The 1,2- or 3,4-dihydro compounds thus obtained can be reduced at the electrode into 1,2,3,4-tetrahydro derivatives.
Stable chromosomal integration of the entire nitrogen fixation gene cluster from Klebsiella pneumoniae in yeast. (1981)

A bacterial plasmid containing the entire nitrogen fixation (nif) gene cluster (consisting of at least 15 genes) from Klebsiella pneumoniae was used in conjunction with an Escherichia coli-yeast shuttle plasmid containing the yeast his4 gene cluster to cotransform a his4- recipient strain of Saccharomyces cerevisiae. Of 87 histidine-independent clones screened, 2 contained nif DNA. Restriction and hybridization analyses showed that two copies of the nif plasmid (46 kilobases each) are integrated in tandem in the recipient chromosome by recombination between homologous regions in the transforming plasmids. Chromosomal integration was also verified by tetrad analysis, showing that the nif DNA behaved in meiosis like a Mendelian element. During mitotic growth, one of the two copies of the nif region is frequently lost. The remaining copy of nif is stable, even after 40 generations in nonselective medium.
[Cannabis poisoning after eating salad]. (1997)

We describe 4 patients who suffered gastrointestinal disorders and psychological effects after eating salad prepared with hemp seed oil. The concentration of tetrahydrocannabinol (THC) in this oil far exceeded the recommended tolerance dose. Our observations prompted the Swiss Federal Office of Public Health to publish warnings in the press concerning consumption of this oil. We describe the symptoms of orally ingested THC and point out unresolved problems connected with food containing hemp.
The use of osseous suture anchors in the treatment of severe, complicated elbow dislocations. (1996)

Seventeen patients who sustained severe trauma resulting in dislocation or fracture-dislocation of the elbow were treated using osseous suture anchors to repair the soft-tissue constraints of the elbow. In 15 of these patients, the medial collateral ligament and flexor-pronator origin were repaired. Ten patients underwent repair of the lateral collateral ligament using anchors. Five patients were also treated with a hinged external fixator. All of the elbows were rendered grossly unstable by the injury; all of the patients had stable elbows at follow-up. Elbow flexion averaged 127 degrees; an average 19 degrees extension loss was noted. The arc of forearm rotation averaged 156 degrees. The aggressive approach detailed in this report is applicable only to severe injuries to the elbow, not routine dislocations. These cases demonstrate the reliability of the osseous suture anchor in the operative treatment of massive trauma to the elbow.
Detecting hardware trojans using backside optical imaging of embedded watermarks (2015)

Hardware Trojans are a critical security threat to integrated circuits. We propose an optical method to detect and localize Trojans inserted during the chip fabrication stage. We engineer the fill cells in a standard cell library to be highly reflective at near-IR wavelengths so that they can be readily observed in an optical image taken through the backside of the chip. The pattern produced by their locations produces an easily measured watermark of the circuit layout. Replacement, modification or re-arrangement of these cells to add a Trojan can therefore be detected through rapid post-fabrication backside imaging. We evaluate our approach using various hardware blocks where the Trojan circuit area is less than 0.1% of the total area and it consumes less than 2% leakage power of the entire chip. In addition, we evaluate the tolerance of our methodology to background measurement noise and process variation.
CYTOKINE RELEASE BY PERIPHERAL BLOOD MONONUCLEAR CELLS IS AFFECTED BY 8‐METHOXYPSORALEN PLUS UV‐A (1994)

Psoralen plus UV-A (PUVA) is an effective therapy for psoriasis but also for other inflammatory dermatoses. The precise mechanisms of action, however, are not absolutely clear. Therefore, the effect of PUVA on the release of the proinflammatory cytokines interleukin (IL)-1, IL-6, IL-8 and tumor necrosis factor alpha (TNF alpha) was studied. Peripheral blood mononuclear cells (PBMC) obtained from humans were incubated with 8-methoxypsoralen (8-MOP) and exposed to UV-A (20 kJ/m2). This treatment resulted in a significant reduction of IL-6 and IL-8 amounts in the supernatants. In addition, an inhibition of IL-1 beta and TNF alpha production by lipopolysaccharide (LPS)-stimulated PBMC was observed upon PUVA treatment. Accordingly, northern blot analysis showed decreased levels of mRNA encoding for IL-1 beta, IL-6, IL-8 and TNF alpha in PUVA-treated PBMC. Finally PBMC were obtained from psoriatics undergoing oral photochemotherapy before the beginning and after completion of treatment. The PBMC collected after PUVA spontaneously produced significantly less IL-6 and IL-8 in comparison to the respective samples obtained before therapy. A similar suppression of IL-1 beta and TNF alpha by in vivo PUVA was found in LPS-stimulated PBMC. The present data demonstrate that PUVA both in vitro and in vivo suppresses the production of the proinflammatory cytokines IL-1 beta, IL-6, IL-8 and TNF alpha by PBMC. Because these cytokines are important in the mediation of inflammatory reactions, one may speculate that the inhibitory effects could contribute to the antiinflammatory activity of PUVA.
Alternative Synthesis of the PDE5 Inhibitor RWJ387273 (R290629) (2007)

An alternative synthesis of a PDE5 inhibitor is reported using a highly diastereoselective Pictet-Spengler reaction.
A Casein Kinase I Activity Is Constitutively Associated with Nck (1997)

Nck is a 47-kDa cytosolic protein devoid of intrinsic catalytic activity and consisting of Src homology 2 and 3 (SH2 and SH3) domains organized as follows: SH3-SH3-SH3-SH2. Nck is believed to act as an adaptor protein mediating signal transduction initiated by receptor tyrosine kinases (RTKs). Through its SH2 domain, Nck recognizes a specific phosphotyrosine residue on RTKs or on protein substrates of RTKs like insulin receptor substrate-1, the major substrate of the insulin receptor, and through its SH3 domains it interacts with poorly characterized effector molecules. To identify novel proteins that might interact with Nck, we have used the amino-terminal segment of Nck encompassing its three SH3 domains in the yeast two-hybrid system. Among the polypeptides that associate with Nck, we have identified the gamma2 isoform of the serine/threonine casein kinase I (CKI-gamma2). In transformed rat hepatocytes overexpressing the insulin receptor (HTC-IR cells), serine/threonine protein kinase activity coimmunoprecipitates with Nck, an interaction mediated mainly by the third SH3 domain of Nck. This kinase activity is not apparently modulated by insulin, nor is it sensitive to staurosporine or heparin, and it does not use GTP as a phosphate donor. However the kinase activity coimmunoprecipitated with Nck is completely abolished by N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide, a specific inhibitor of casein kinase I. In an in vitro renaturation gel kinase assay, a protein kinase of 70-75 kDa was detected associated with the SH3 domains of Nck. Far Western analysis demonstrated that the SH3 domains of Nck bound directly to a cytosolic protein of 70-75 kDa. A rabbit polyclonal antibody raised against the C-terminal region of CKI-gamma2 protein kinase immunoprecipitated a single specific protein of 70-75 kDa from HTC-IR cell lysates and detected CKI-gamma2 among the proteins coimmunoprecipitated with Nck. These results support an in vivo interaction between Nck and CKI-gamma2 and suggest that CKI-gamma2 could be involved in signaling pathways downstream of RTKs.
Performance analysis of linear joint multiple access interference cancellation-equalization for asynchronous multitone CDMA (2002)

We analyze the problem of joint equalization and cancellation of the multipath and multiple access interference for multitone CDMA introduced by Vandendorpe (see IEEE Transactions on Vehicular Technology, vol.44, no. 2, p.327, 1995). A linear structure is investigated for an MMSE criterion. In order to understand the potential of the system we first study the steady-state behaviour of the joint equalizer-canceller. The investigation is carried out for a joint device following a receiver made of a bank of matched filters and assuming perfect carrier phase and timing recovery. The scenario is asynchronous. An exact expression in the form of an integral is obtained for the bit error probability of the BPSK symbols before and after cancellation, by means of the characteristic function method. The performance of different systems is obtained for a constrained comparison and the near-far resistance of the detector is demonstrated.
Compressive sensing computational ghost imaging (2012)

The computational ghost imaging with a phase spatial light modulator (SLM) for wave field coding is considered. A transmission-mask amplitude object is reconstructed from multiple intensity observations. Compressive techniques are used in order to gain a successful image reconstruction with a number of observations (measurement experiments), which is smaller than the image size. Maximum likelihood style algorithms are developed, respectively, for Poissonian and approximate Gaussian modeling of random observations. A sparse and overcomplete modeling of the object enables the advanced high accuracy and sharp imaging. Numerical experiments demonstrate that an approximative Gaussian distribution with an invariant variance results in the algorithm that is efficient for Poissonian observations.
Engineered Knottin Peptides: A New Class of Agents for Imaging Integrin Expression in Living Subjects (2009)

There is a critical need for molecular imaging agents to detect cell surface integrin receptors that are present in human cancers. Previously, we used directed evolution to engineer knottin peptides that bind with high affinity ( approximately 10 to 30 nmol/L) to integrin receptors that are overexpressed on the surface of tumor cells and the tumor neovasculature. To evaluate these peptides as molecular imaging agents, we site-specifically conjugated Cy5.5 or (64)Cu-1,4,7,10-tetra-azacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA) to their N termini, and used optical and positron emission tomography (PET) imaging to measure their uptake and biodistribution in U87MG glioblastoma murine xenograft models. NIR fluorescence and microPET imaging both showed that integrin binding affinity plays a strong role in the tumor uptake of knottin peptides. Tumor uptake at 1 hour postinjection for two high-affinity (IC(50), approximately 20 nmol/L) (64)Cu-DOTA-conjugated knottin peptides was 4.47% +/- 1.21% and 4.56% +/- 0.64% injected dose/gram (%ID/g), compared with a low-affinity knottin peptide (IC(50), approximately 0.4 mumol/L; 1.48 +/- 0.53%ID/g) and c(RGDyK) (IC(50), approximately 1 mumol/L; 2.32 +/- 0.55%ID/g), a low-affinity cyclic pentapeptide under clinical development. Furthermore, (64)Cu-DOTA-conjugated knottin peptides generated lower levels of nonspecific liver uptake ( approximately 2%ID/g) compared with c(RGDyK) ( approximately 4%ID/g) 1 hour postinjection. MicroPET imaging results were confirmed by in vivo biodistribution studies. (64)Cu-DOTA-conjugated knottin peptides were stable in mouse serum, and in vivo metabolite analysis showed minimal degradation in the blood or tumor upon injection. Thus, engineered integrin-binding knottin peptides show great potential as clinical diagnostics for a variety of cancers.
Long-term stabilization in patients with malignant glioma after treatment with liposomal doxorubicin (2001)

Doxorubicin has been shown to be a safe treatment with moderate activity that may lead to long-term stabilization in recurrent high-grade glioma patients. Of note, median OS after all and after initiation of recurrence therapy was prolonged in comparison with the OS in other Phase II studies, as recently described by Wong et al. (Wong ET, Hess KR, Gleason MJ, Jaeckle KA, Kyritsis AP, Prados MD, et al. Outcomes and prognostic factors in recurrent glioma patients enrolled onto phase II clinical trials. J Clin Oncol 1999;17:2572.).
Regulation of chromatin structure by poly(ADP-ribosyl)ation (2012)

The interaction of DNA with proteins in the context of chromatin has to be tightly regulated to achieve so different tasks as packaging, transcription, replication and repair. The very rapid and transient post-translational modification of proteins by poly(ADP-ribose) has been shown to take part in all four. Originally identified as immediate cellular answer to a variety of genotoxic stresses, already early data indicated the ability of this highly charged nucleic acid-like polymer to modulate nucleosome structure, the basic unit of chromatin. At the same time the enzyme responsible for synthesizing poly(ADP-ribose), the zinc-finger protein poly(ADP-ribose) polymerase-1 (PARP1), was shown to control transcription initiation as basic factor TFIIC within the RNA-polymerase II machinery. Later research focused more on PARP-mediated regulation of DNA repair and cell death, but in the last few years, transcription as well as chromatin modulation has re-appeared on the scene. This review will discuss the impact of PARP1 on transcription and transcription factors, its implication in chromatin remodeling for DNA repair and probably also replication, and its role in controlling epigenetic events such as DNA methylation and the functionality of the insulator protein CCCTC-binding factor.
A novel voltage-clamped CMOS ISFET sensor interface (2007)

This paper presents a novel interface for ion-sensitive field effect transistors (ISFET) in which operation at a fixed electrical bias is achieved by voltage clamping. The chosen topology provides pH-dependent current and voltage output signals to drive an appropriate output stage. The circuit can be operated in either strong or weak inversion, depending on the requirements of the application with a pseudo-differential ISFET-REFET topology to allow use of an on-chip reference electrode. Simulation results are shown herein for single-ended and differential implementations. For a single-ended front-end with 1nA bias current, the strong inversion implementation at 2.5V supply has a power consumption of 0.185mW at pH 7 and the weak inversion implementation at 1.5V supply has a power consumption of 13nW at pH 7. The circuit and ISFET-REFET pair have been fabricated in the UMC 0.25 <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">μ</i> m CMOS technology.
Ru complexes bearing bidentate carbenes: from innocent curiosity to uniquely effective catalysts for olefin metathesis (2003)

The discovery and development of a new class of Ru-based catalysts for olefin metathesis is described. These catalysts, particularly those that do not bear a phosphine ligand, have been demonstrated to promote unique levels of reactivity in a variety of olefin metathesis reactions. The design and development of supported and chiral optically pure variants of this class of Ru catalysts for use in enantioselective metathesis are discussed as well. All catalysts are air stable, reusable, and can be employed with unpurified solvents.