gebhart/patrepeval huggingface dataset:
ATOMISTIC SIMULATION OF SEGREGATION IN Cu-Bi SYSTEM II. SEGREGATION INDUCED STRUCTURAL TRANSFORMATIONS AT GRAIN BOUNDARIES (1988)

Atomic structures of.four Σ=17(530)/[001]symmetrical boundaries in Cu con- taining segregated Bi atoms at different sites have been studied.The segregation induced structural transformations frorn one structure to another accompanied by the displace- ment of the upper grain in respect to the lower grain and the migration of the grain boundary plane have been observed.A shear mechanism has been proposed in order to explain such findings on the basis of the grain boundary,structural unit model and the structural multiplicity.
Neural Stem Cell Transplantation Induces Stroke Recovery by Upregulating Glutamate Transporter GLT-1 in Astrocytes (2016)

Tissue damage and loss of function occurring after stroke can be constrained by fostering plasticity processes of the brain. Over the past years, stem cell transplantation for repair of the CNS has received increasing interest, although underlying mechanism remain elusive. We here show that neural stem/precursor cell transplantation after ischemic stroke is able to foster axonal rewiring and dendritic plasticity and to induce long-term functional recovery. The observed therapeutic effect of neural precursor cells seems to underlie their capacity to upregulate the glial glutamate transporter on astrocytes through the vascular endothelial growth factor inducing favorable changes in the electrical and molecular stroke microenvironment. Cell-based approaches able to influence plasticity seem particularly suited to favor poststroke recovery.
Biopsy of Preimplantation Mouse Embryos: Development of Micromanipulated Embryos and Proliferation of Single Blastomeres in Vitro1 (1989)

We have developed a technique to sample the preimplantation embryo, which may, in the future, be applied to prenatal diagnosis of genetic disease. Using micromanipulation, we aspirated a single blastomere from 4-cell mouse embryos. This procedure had no effect on in vitro development; 98% of control and 94% of biopsied embryos reached the blastocyst stage after 48 h in culture. Furthermore, after transfer to pseudopregnant recipient mice, the rate of fetal development of biopsied embryos was not significantly different from control embryos, although implantation rate was significantly reduced (mean +/- SD: biopsied 53.1 +/- 4.0, control 81.8 +/- 8.4, p less than 0.001). For the first time we have produced monolayer cell cultures derived from single preimplantation blastomeres. Individual biopsied blastomeres were cultured in vitro on different extracellular matrix components. Significantly greater cell proliferation was obtained in wells coated with fibronectin (FN), laminin (LN), and a complex of laminin and nidogen (LNC) than in a less specific matrix of swine skin gelatin (SSG). Mean (+/- SE) cell nuclei number per well after 6 days in culture was 6.4 +/- 2.1, 11.9 +/- 1.5, 19.8 +/- 2.9, and 20.9 +/- 2.6 in wells coated with SSG, LN, FN, and LNC respectively.
ZnO nanowire field-effect transistor and oxygen sensing property (2004)

Single-crystal ZnO nanowires are synthesized using a vapor trapping chemical vapor deposition method and configured as field-effect transistors. Electrical transport studies show n-type semiconducting behavior with a carrier concentration of ∼107cm−1 and an electron mobility of ∼17cm2∕Vs. The contact Schottky barrier between the Au/Ni electrode and nanowire is determined from the temperature dependence of the conductance. Thermionic emission is found to dominate the transport mechanism. The effect of oxygen adsorption on electron transport through the nanowires is investigated. The sensitivity to oxygen is demonstrated to be higher with smaller radii nanowires. Moreover, the oxygen detection sensitivity can be modulated by the gate voltage. These results indicate that ZnO holds high potential for nanoscale sensing applications.
High-Density Lipoprotein Cholesterol and Risk of Ischemic Stroke Mortality (1997)

In this prospective study of middle-aged and elderly men from a healthy, working population, we have demonstrated an independent negative association between HDL-C and ischemic stroke mortality during a long-term (21-year) follow-up.
Versatile Raman fiber laser for sodium laser guide star (2014)

Abstract Robust high‐power narrow‐linewidth lasers at 589 nm are required for sodium laser guide star adaptive optics in astronomy. A high‐power 589 nm laser based on Raman fiber amplifier is reported here, which works in both continuous‐wave and pulsed formats. In the continuous‐wave case, the laser produces more than 50 W output. In the pulsed case, the same laser produces square‐shaped pulses with tunable repetition rate (500 Hz to 10 kHz) and duration (1 ms to 30 μs). The peak power is as high as 84 W and remains constant during the tuning. The laser also emits an adjustable sideband at 1.71 GHz away from the main laser frequency for better sodium excitation. The versatility of the laser offers much flexibility in laser guide star application.
Cloning and characterization of a gene encoding phosphoketolase in a Lactobacillus paraplantarum isolated from Kimchi. (2007)

A gene coding for phosphoketolase, a key enzyme of carbohydrate catabolism in heterofermentative lactic acid bacteria (LAB), was cloned from a Lactobacillus paraplantarum C7 and expressed in Escherichia coli. The gene is 2502 bp long and codes for a 788-amino-acids polypeptide with a molecular mass of 88.7 kDa. A Shine-Dalgamo sequence (aaggag) and an inverted-repeat terminator sequence are located upstream and downstream of the phosphoketolase gene, respectively. The gene exhibits an identity of >52% with phosphoketolases of other LAB. The phosphoketolase of Lb. paraplantarum C7 (LBPK) contains several highly conserved phosphoketolase signature regions and typical thiamine pyrophosphate (TPP) binding sites, as reported for other TPP-dependent enzymes. The phosphoketolase gene was fused to a glutathione S-transferase (GST::LBPK) gene for purification. The GST::LBPK fusion protein was detected in the soluble fraction of a recombinant Escherichia coli BL21. The GST::LBPK fusion protein was purified with a yield of 4.32 mg/400 ml by GSTrap HP affinity column chromatography and analyzed by N-terminal sequencing. LBPK was obtained by factor Xa treatment of fusion protein and the final yield was 3.78 mg/400 ml. LBPK was examined for its N-terminal sequence and phosphoketolase activity. The K(M) and Vmax values for fructose-6-phosphate were 5.08 +/- 0.057 mM (mean +/- SD) and 499.21 +/- 4.33 micromol/min/mg, respectively, and the optimum temperature and pH for the production of acetyl phosphate were 45 degrees C and 7.0, respectively.
Collaborative signal processing for action recognition in body sensor networks (2010)

Body sensor networks are emerging as a promising platform for remote human monitoring. With the aim of extracting bio-kinematic parameters from distributed body-worn sensors, these systems require collaboration of sensor nodes to obtain relevant information from an overwhelmingly large volume of data. Clearly, efficient data reduction techniques and distributed signal processing algorithms are needed. In this paper, we present a data processing technique that constructs motion transcripts from inertial sensors and identifies human movements by taking collaboration between the nodes into consideration. Transcripts of basic motions, called primitives, are built to reduce the complexity of the sensor data. This model leads to a distributed algorithm for segmentation and action recognition. We demonstrate the effectiveness of our framework using data collected from five normal subjects performing ten transitional movements. The results clearly illustrate the effectiveness of our framework. In particular, we obtain a classification accuracy of 84.13% with only one sensor node involved in the classification process.
Knowledge-based support for scientific programming (2003)

The author considers how knowledge-based tools can support scientific computing, with a focus on the support of code generation. Scientific computing traditionally is carried out by mathematical modelers who write their own Fortran programs. As faster machines and new architectures make more complex problems computationally feasible, programming becomes more cost effective. Scientific computing comprises a variety of activities including model formulation, coding, and interpretation. The SINAPSE program synthesis system illustrates the use of knowledge-based techniques in model solution. SINAPSE is designed to be part of a problem-solving environment for forward modeling that includes several additional scientific programming activities. SINAPSE generates code by applying refinements and optimizing transformations, first producing an algorithm description and then an array-based, high-level language program before generating target code. SINAPSE generates implementations targeted toward a specified architecture.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. (1995)

The importance of the B7/CD28/CTLA-4 molecules has been established in studies of antigen-presenting cell-derived B7 and its interaction with the T cell costimulatory molecule CD28. CTLA-4, a T cell surface glycoprotein that is related to CD28, can also interact with B7-1 and B7-2. However, less is known about the function of CTLA-4, which is expressed at highest levels after activation. We have generated an antibody to CTLA-4 to investigate the consequences of engagement of this molecule in a carefully defined system using highly purified T cells. We show here that the presence of low levels of B7-2 on freshly explanted T cells can partially inhibit T cell proliferation, and this inhibition is mediated by interactions with CTLA-4. Cross-linking of CTLA-4 together with the TCR and CD28 strongly inhibits proliferation and IL-2 secretion by T cells. Finally, results show that CD28 and CTLA-4 deliver opposing signals that appear to be integrated by the T cell in determining the response to activation. These data strongly suggest that the outcome of T cell antigen receptor stimulation is regulated by CD28 costimulatory signals, as well as inhibitory signals derived from CTLA-4.
Antimicrobial activity of iron oxide nanoparticle upon modulation of nanoparticle-bacteria interface (2015)

Abstract Investigating the interaction patterns at nano-bio interface is a key challenge for safe use of nanoparticles (NPs) to any biological system. The study intends to explore the role of interaction pattern at the iron oxide nanoparticle (IONP)-bacteria interface affecting antimicrobial propensity of IONP. To this end, IONP with magnetite like atomic arrangement and negative surface potential (n-IONP) was synthesized by co-precipitation method. Positively charged chitosan molecule coating was used to reverse the surface potential of n-IONP, i.e. positive surface potential IONP (p-IONP). The comparative data from fourier transform infrared spectroscope, XRD and zeta potential analyzer indicated the successful coating of IONP surface with chitosan molecule. Additionally, the nanocrystals obtained were found to have spherical size with 10–20 nm diameter. The BacLight fluorescence assay, bacterial growth kinetic and colony forming unit studies indicated that n-IONP (&lt;50 μM) has insignificant antimicrobial activity against Bacillus subtilis and Escherichia coli . However, coating with chitosan molecule resulted significant increase in antimicrobial propensity of IONP. Additionally, the assay to study reactive oxygen species (ROS) indicated relatively higher ROS production upon p-IONP treatment of the bacteria. The data, altogether, indicated that the chitosan coating of IONP result in interface that enhances ROS production, hence the antimicrobial activity.
New Organic Materials Suitable for Use in Chemical Sensor Arrays (1998)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Organic Materials Suitable for Use in Chemical Sensor ArraysRichard M. Crooks and Antonio J. RiccoView Author Information Department of Chemistry, Texas A&M University, P.O. Box 300012, College Station, Texas 77842 Microsensor R&D Department, Sandia National Laboratories, Albuquerque, New Mexico 87185-1425 Cite this: Acc. Chem. Res. 1998, 31, 5, 219–227Publication Date (Web):April 17, 1998Publication History Received20 August 1997Published online17 April 1998Published inissue 1 May 1998https://pubs.acs.org/doi/10.1021/ar970246hhttps://doi.org/10.1021/ar970246hresearch-articleACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views1424Altmetric-Citations249LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Aromatic compounds,Dendrons,Hydrocarbons,Materials,Monolayers Get e-Alerts
Protein Kinase C Inhibitors Suppress Cell Growth in Established and Low-Passage Glioma Cell Lines. A Comparison between Staurosporine and Tamoxifen (1993)

WE HAVE PREVIOUSLY demonstrated that the proliferation of established human glioma cell lines correlated with protein kinase C (PKC) activity and that a relatively selective PKC inhibitor, staurosporine, inhibits glioma cell proliferation. The purpose of this study was to determine whether low-passage glioma cell lines were also sensitive to staurosporine and to compare the antimitotic effects of staurosporine with tamoxifen, an antiestrogen with a known PKC inhibitory effect presently being investigated in the treatment of recurrent glioma. We measured the effects of treatment with staurosporine or tamoxifen on the proliferation rate of five established glioma cell lines (A172, U251, U87, U373, U563) and four low-passage glioma cell lines. The proliferation of all cell lines was inhibited by staurosporine, at an IC50 value (concentration at which activity is 50% inhibited) of approximately 2 nmol/L. All established lines, but only one low-passage line, were susceptible to tamoxifen, with an IC50 value of 10 μmol/L. Three of the four low-passage lines were poorly inhibited by tamoxifen. The IC50 values for the inhibition of cellular proliferation by staurosporine and tamoxifen closely corresponds to the IC50 data for the inhibition of particulate PKC activity in gliomas. We conclude that staurosporine is more effective in the inhibition of glioma proliferation than tamoxifen and that staurosporine is potentially useful in the adjuvant treatment of gliomas. The correspondence in IC50 results for proliferation and PKC activity further strengthens the hypothesis that an aberrant PKC system in gliomas drives their hyperproliferative state.
Sr- and Mn-doped LaAlO3−δ for solar thermochemical H2 and CO production (2013)

The increasing global appetite for energy within the transportation sector will inevitably result in the combustion of more fossil fuel. A renewable-derived approach to carbon-neutral synthetic fuels is therefore needed to offset the negative impacts of this trend, which include climate change. In this communication we report the use of nonstoichiometric perovskite oxides in two-step, solar-thermochemical water or carbon dioxide splitting cycles. We find that LaAlO3 doped with Mn and Sr will efficiently split both gases. Moreover the H2 yields are 9× greater, and the CO yields 6× greater, than those produced by the current state-of-the-art material, ceria, when reduced at 1350 °C and re-oxidized at 1000 °C. The temperature at which O2 begins to evolve from the perovskite is fully 300 °C below that of ceria. The materials are also very robust, maintaining their redox activity over at least 80 CO2 splitting cycles. This discovery has profound implications for the development of concentrated solar fuel technologies.
Subcellular localization of neural‐specific NPDC‐1 protein (2005)

NPDC-1 is a gene specifically expressed in neural cells when they stop to divide and begin to differentiate. Immunocytochemical study analysis of differentiated PC12 cells transfected with NPDC-tag vectors showed that NPDC-1 is transported in vesicles from the Golgi apparatus to the cell membrane and is then likely internalized into endosomes. The protein colocalized, at least partially, with synaptic vesicle proteins: synaptophysin, synaptobrevin 2, and Rab3 GEP (Rab3 GTP/GDP exchange protein). Moreover, subcellular fractionation of rat brain showed that crude synaptic membrane and crude synaptic vesicle fractions were enriched in NPDC-1. Although NPDC-1 bound Rab3 GEP in vitro, it seems unlikely to be involved in Ca2+-dependent exocytosis and, thus, in synaptic vesicle trafficking.
[Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition]. (2007)

Serial ultracentrifugation and sugar density ultracentrifugation can be used to purify H22 cell-derived exosomes. H22 cell-derived exosomes express a distinct set of proteins involving in and/or relating to antigen presentation (HSP70, ICAM-1), migration (DLC-A), adhesion (ICAM-1), cytoskeleton (EF-G2) and tumour antigens (C-myc, Vav-2). The exosomes have immunogenicity.
Growth Hormone Induction of Hepatic Serine Protease Inhibitor 2.1 Transcription Is Mediated by a Stat5-related Factor Binding Synergistically to Two γ-Activated Sites (1995)

A growth hormone (GH)-inducible nuclear factor (GHINF) from rat liver has been purified to near homogeneity. On SDS-polyacrylamide gel electrophoresis and UV-cross-linking, a major band of mass approximately 93 kDa and a minor band of approximately 70 kDa are detected in the purified fraction. DNase I footprinting using purified GHINF yields a protected region of -149/-115 on the rat serine protease inhibitor 2.1 (Spi 2.1) promoter encompassed within the growth hormone response element (GHRE). Mutational analysis demonstrated that GHINF binds synergistically to two gamma-interferon-activated sites (GAS) within the GHRE, with the 3' element being the pivotal binding domain. Functional assays show that both GAS elements are necessary for full GH response. GHINF has no immunoreactivity with either a C-terminal Stat1 antibody or an N-terminal Stat3 antibody, while cross-reacting with a C-terminal Stat5 monoclonal antibody. GHINF will bind to two GAS elements from the Stat5 binding region of the beta-casein gene. These studies indicate that GHINF is a Stat5-related factor binding synergistically to two GAS elements to activate Spi 2.1 transcription.
The Inhibitory Effect of Essential Oils on Herpes Simplex Virus Type‐1 Replication In Vitro (2003)

The antiviral effect of 12 essential oils on herpes simplex virus type-1 (HSV-1) replication was examined in vitro. The replication ability of HSV-1 was suppressed by incubation of HSV-1 with 1% essential oils at 4 C for 24 hr. Especially, lemongrass completely inhibited the viral replication even at a concentration of 0.1%, and its antiviral activity was dependent on the concentrations of the essential oil. When Vero cells were treated with the essential oil before or after viral adsorption, no antiviral activity was found, which suggests that the antiviral activity of essential oils including lemongrass may be due to the direct interaction with virions.
Nanometer scale patterning of silicon (100) surfaces by an atomic force microscope operating in air (1994)

Modification on silicon (100) surfaces was demonstrated by using an atomic force microscope operating in air. Field-enhanced oxidation on silicon surfaces with protection oxide was done locally by biasing a p-type heavily doped silicon tip between −3 and −10 V. Oxide lines of width as small as ∼10 nm were achieved. After a dip in aqueous HF solution, the oxide was etched away; the modification depth, ∼1 nm, was characterized by the same atomic force microscope. Other field induced reactions for patterning are possible.
At the edge of translation – materials to program cells for directed differentiation (2010)

Oral Diseases (2011) 17, 241-251 The rapid advancement in basic biology knowledge, especially in the stem cell field, has created new opportunities to develop biomaterials capable of orchestrating the behavior of transplanted and host cells. Based on our current understanding of cellular differentiation, a conceptual framework for the use of materials to program cells in situ is presented, namely a domino vs a switchboard model, to highlight the use of single vs multiple cues in a controlled manner to modulate biological processes. Further, specific design principles of material systems to present soluble and insoluble cues that are capable of recruiting, programming and deploying host cells for various applications are presented. The evolution of biomaterials from simple inert substances used to fill defects, to the recent development of sophisticated material systems capable of programming cells in situ is providing a platform to translate our understanding of basic biological mechanisms to clinical care.
Electrooptical effects in silicon (1987)

A numerical Kramers-Kronig analysis is used to predict the refractive-index perturbations produced in crystalline silicon by applied electric fields or by charge carriers. Results are obtained over the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1.0-2.0 \mu</tex> m optical wavelength range. The analysis makes use of experimental electroabsorption spectra and impurity-doping spectra taken from the literature. For electrorefraction at the indirect gap, we find <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\Delta n = 1.3 \times 10^{5}</tex> at <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\lambda = 1.07 \mu</tex> m when <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">E = 10^{5}</tex> V/cm, while the Kerr effect gives <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\Delta n = 10^{-6}</tex> at that field strength. The charge-carrier effects are larger, and a depletion or injection of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">18</sup> carriers/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> produces an index change of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\pm1.5 \times 10^{-3}</tex> at <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\lambda = 1.3 \mu</tex> m.