gebhart/patrepeval huggingface dataset:
T Cytotoxic-1 CD8+ T Cells Are Effector Cells against Pneumocystis in Mice (2004)

Host defenses are profoundly compromised in HIV-infected hosts due to progressive depletion of CD4+ T lymphocytes. A hallmark of HIV infection is Pneumocystis carinii (PC) pneumonia. Recently, CD8+ T cells, which are recruited to the lung in large numbers in response to PC infection, have been associated with some level of host defense as well as contributing to lung injury in BALB/c mice. In this study, we show that CD8+ T cells that have a T cytotoxic-1 response to PC in BALB/c mice, as determined by secretion of IFN-gamma, have in vitro killing activity against PC and effect clearance of the organism in adoptive transfer studies. Moreover, non-T cytotoxic-1 CD8+ T cells lacked in vitro effector activity and contributed to lung injury upon adoptive transfer. This dichotomous response in CD8+ T cell response may in part explain the clinical heterogeneity in the severity of PC pneumonia.
An architecture for wide-area multicast routing (1994)

Existing multicast routing mechanisms were intended for use within regions where a group is widely represented or bandwidth is universally plentiful. When group members, and senders to those group members, are distributed sparsely across a wide area, these schemes are not efficient; data packets or membership report information are occasionally sent over many links that do not lead to receivers or senders, respectively. We have developed a multicast routing architecture that efficiently establishes distribution trees across wide area internets, where many groups will be sparsely represented. Efficiency is measured in terms of the state, control message processing, and data packet processing, required across the entire network in order to deliver data packets to the members of the group. Our Protocol Independent Multicast (PIM) architecture: (a) maintains the traditional IP multicast service model of receiver-initiated membership; (b) can be configured to adapt to different multicast group and network characteristics; (c) is not dependent on a specific unicast routing protocol; and (d) uses soft-state mechanisms to adapt to underlying network conditions and group dynamics. The robustness, flexibility, and scaling properties of this architecture make it well suited to large heterogeneous inter-networks.
STCP-1 (MDC) CC Chemokine Acts Specifically on Chronically Activated Th2 Lymphocytes and Is Produced by Monocytes on Stimulation with Th2 Cytokines IL-4 and IL-13 (1998)

STCP-1 stimulated T cell chemoattractant protein-1 (STCP-1) (macrophage-derived chemokine; MDC), a recently described CC chemokine for chronically activated T lymphocytes, was found to act specifically on a subset of memory CD4 lymphocytes that displayed a Th2 cytokine profile. Also, STCP-1, thymus and activation regulated chemokine (TARC), eotaxin, and eotaxin-2 acted specifically on in vitro derived Th2 lymphocytes, while IP-10 (IFN-gamma-inducible 10-kDa protein) showed some preference for Th1 lymphocytes. The corresponding receptors for eotaxin, TARC, and IP-10 are also differentially expressed on Th1 and Th2 lymphocytes. In desensitization Ca flux experiments, TARC and STCP-1 bound to a common receptor and therefore at least one chemokine receptor for STCP-1 is CCR4. STCP-1 expression is restricted to immune cells. Dendritic cells, B cells, and macrophages produce STCP-1 constitutively, while NK cells, monocytes, and CD4 lymphocytes produce STCP-1 upon appropriate stimulation. Production of STCP-1 is positively modulated by Th2 cytokines IL-4 and IL-13 but inhibited by IL-10.
Surface site specificity on the basal plane of graphite: 1.06 μm laser damage threshold and reactivity with oxygen between 350 and 2300 K (1991)

Scanning tunneling microscopy (STM) has been used to document changes in the nanometer-scale morphology of the basal plane of highly oriented pyrolytic graphite after in situ exposure to 7 ns, 1064 nm Nd:YAG, and to ms Nd:Glass laser pulses producing surface temperatures up to 2300 K, and after ex situ exposure to temperatures as low as 350 K in air. Laser damage produced by the ns pulses was visible by STM at fluences far below those that produce melting and effects visible by other imaging techniques. Damage appears first on step edges and consists of exfoliation of graphite layers and recession of steps through removal of mono- or multilayer patches. ms and long-term heating leads to reaction with oxygen, which proceeds by etching of both crystalline boundaries and step edges. In addition a high concentration of flat-bottom shallow pits (two monolayers deep) and a smaller number of conical pits (greater than 30 Å deep) can clearly be identified. The results give for the reaction at the grain boundaries an activation energy of 36 kcal/mole while for steps and shallow pits it is 53 kcal/mole, within the energy range found in global, not site-specific reaction studies. Site-specific reaction probabilities as low as 10−11 are measured at 350 K.
丁玲作品《一天》浅析 (2001)

丁玲在其丈夫胡也频及其他左翼文艺家的影响下，逐渐积极投身于革命运动。随着她的向左转，她的小说从内容到形式都发生了变化。短篇小说《一天》，就创作于这一变化过程中间。作品描述了女主人公的个人心理，同时也剖析了当时困扰着中国社会的各种邪恶现实。
Ex vivo expansion of hematopoietic stem cells: mission accomplished? (2011)

A small number of hematopoietic stem cells (HSCs) with self-renewal and multi-lineage repopulation capacity maintain hematopoiesis during the lifetime of an individual. Moreover, HSCs and their potential exist in excess as one individual can share its HSCs with another leading to creation of a genetically identical hematopoietic system. For over half a century this property of HSCs has been utilised by successful allogeneic clinical HSC transplantation for treatment of patients with inherited or acquired genetic and neoplastic diseases of the hematopoietic and immune system. There are now more than twenty thousand allogeneic HSC transplants per year worldwide [1]. However, although more than 17.5 million potential HSC donors are registered and additional 500,000 cord bloods are stored for potential allogeneic HSC transplantation [2], timely availability of appropriately human leukocyte antigen (HLA)-compatible HSCs with sufficient quality for patients still poses a problem in the field. Even if a donor is available, toxicity of the procedure could be reduced by increasing HSC numbers in transplants. One way to solve these issues would be by generation of quality-controlled, off the shelf HSC products via in vitro HSC expansion, a "holy grail" procedure many have been hunting for. Here, we discuss accumulating knowledge on signalling pathways involved in HSC maintenance as well as recent achievements to apply the findings to ex vivo HSC expansion for clinical use. Although the specific issue concerns only highly specialised medicine today, newly generated knowledge will be critical for the whole field of stem cell transplantation and regenerative medicine in the future.
What might the psychobiology of posttraumatic stress disorder teach us about future approaches to pharmacotherapy? (2000)

This review considers future directions for developing effective drugs for posttraumatic stress disorder (PTSD). At present, we have embarked upon an empirical approach in which pharmacologic research consists of clinical trials with agents, such as antidepressants, anxiolytics, and anticonvulsants, initially developed for different purposes. The approach taken here is theoretical rather than empirical, starting with what is known about the unique pathophysiology of PTSD and then predicting the types of pharmacologic agents that might prove effective in the future. Such classes of compounds include corticotropin-releasing factor antagonists, neuropeptide Y enhancers, antiadrenergic compounds, drugs to down-regulate glucocorticoid receptors, more specific serotonergic agents, agents normalizing opioid function, substance P antagonists, N-methyl-D-aspartate facilitators, and antikindling/antisensitization anticonvulsants.
Maitotoxin and P2Z/P2X7purinergic receptor stimulation activate a common cytolytic pore (1999)

The effects of maitotoxin (MTX) on plasmalemma permeability are similar to those caused by stimulation of P2Z/P2X(7) ionotropic receptors, suggesting that 1) MTX directly activates P2Z/P2X(7) receptors or 2) MTX and P2Z/P2X(7) receptor stimulation activate a common cytolytic pore. To distinguish between these two possibilities, the effect of MTX was examined in 1) THP-1 monocytic cells before and after treatment with lipopolysaccharide and interferon-gamma, a maneuver known to upregulate P2Z/P2X(7) receptor, 2) wild-type HEK cells and HEK cells stably expressing the P2Z/P2X(7) receptor, and 3) BW5147.3 lymphoma cells, a cell line that expresses functional P2Z/P2X(7) channels that are poorly linked to pore formation. In control THP-1 monocytes, addition of MTX produced a biphasic increase in the cytosolic free Ca(2+) concentration ([Ca(2+)](i)); the initial increase reflects MTX-induced Ca(2+) influx, whereas the second phase correlates in time with the appearance of large pores and the uptake of ethidium. MTX produced comparable increases in [Ca(2+)](i) and ethidium uptake in THP-1 monocytes overexpressing the P2Z/P2X(7) receptor. In both wild-type HEK and HEK cells stably expressing the P2Z/P2X(7) receptor, MTX-induced increases in [Ca(2+)](i) and ethidium uptake were virtually identical. The response of BW5147.3 cells to concentrations of MTX that produced large increases in [Ca(2+)](i) had no effect on ethidium uptake. In both THP-1 and HEK cells, MTX- and Bz-ATP-induced pores activate with similar kinetics and exhibit similar size exclusion. Last, MTX-induced pore formation, but not channel activation, is greatly attenuated by reducing the temperature to 22 degrees C, a characteristic shared by the P2Z/P2X(7)-induced pore. Together, the results demonstrate that, although MTX activates channels that are distinct from those activated by P2Z/P2X(7) receptor stimulation, the cytolytic/oncotic pores activated by MTX- and Bz-ATP are indistinguishable.
Development of 3T3‐like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40 (1968)

Abstract Balb/c mouse embryo cultures that are carried under a rigid transfer schedule that minimizes cell‐cell contact evolve into permanent lines that possess properties very similar to 3T3. From the same embryo cultures, a transfer schedule where cell‐cell contact is extensive leads to lines (Balb/3T12) that form multiple cell layers and have saturation densities up to 25‐fold higher than the Balb/3T3 lines. All the lines are hypotetraploid. Comparison of the Balb/3T3 lines with 3T3 reveals similar morphology, ability to grow at high dilution, low saturation density, and high susceptibility to transformation by SV40 virus. The Balb/3T12 lines, which are continually maintained at high cell density, show little improvement in cloning efficiency. Infection with SV40 produces transformants that can be selected by their ability to form colonies at low cell densities. Using the appropriate selective system for each line, it appears that SV40 T antigen induction and transformation in Balb/3T3 and Balb/3T12 are comparable.
Evaluation of Apple Maturity with Two Types of Dielectric Probes (2018)

The observed dielectric spectrum of ripe apples in the last period of shelf-life was analyzed using a multipole dielectric relaxation model, which assumes three active relaxation processes: primary α-process (water relaxation) and two secondary processes caused by solid-water-ion interactions α' (bound water relaxations), as well as β' (Maxwell-Wagner effect). The performance of two designs of the dielectric probe was compared: a classical coaxial open-ended probe (OE probe) and an open-ended probe with a prolonged central conductor in a form of an antenna (OE-A-probe). The OE-A probe increases the measurement volume and consequently extends the range of applications to other materials, like granulated agricultural products, soils, or liquid suspensions. However, its measurement frequency range is limited as compared to the OE probe because, above 1.5 GHz, the probe with the antenna generates higher propagation modes and the applied calibrations and calculations are not sufficient. It was shown that data from measurements using the OE-A probe gave slightly stronger correlations with apples' quality parameters than using the typical OE probe. Additionally, we have compared twelve multipole fitting models with different combinations of poles (eight three-pole and four two-pole models). It was shown that the best fit is obtained using a two-pole model for data collected for the OE-A probe and a three-pole model for the OE probe, using only Cole-Cole poles in both cases.
Simultaneous Blocking of Human Toll-Like Receptors 2 and 4 Suppresses Myeloid Dendritic Cell Activation Induced byMycobacterium bovisBacillus Calmette-Guerin Peptidoglycan (2003)

The Mycobacterium bovis bacillus Calmette-Guérin (BCG) cell wall skeleton (CWS) consists of mycolic acids, arabinogalactan, and peptidoglycan (PGN) and activates Toll-like receptor 2 (TLR2) and TLR4. Here we investigated the ability of the essential portion of highly purified BCG CWS to support the TLR agonist function by using the following criteria: myeloid dendritic cell (DC) maturation, i.e., tumor necrosis factor alpha (TNF-alpha) production and CD83/CD86 up-regulation. The purified PGN region was sufficient to activate TLR2 and TLR4 in mouse DCs and macrophages; in TLR2 and TLR4 double-knockout cells the BCG PGN-mediated TNF-alpha production ability was completely impaired. Likewise, stimulation with BCG CWS of HEK293 cells expressing either human TLR2 or TLR4, MD-2, and CD14 resulted in NF-kappa B activation as determined by a reporter assay. Notably, specific blockers of extracellular human TLR2 (an original cocktail of monoclonal antibodies TLR2.45 and TH2.1) and TLR4 (E5531) inhibited BCG CWS-mediated NF-kappa B activation by 80%. Using this human TLR blocking system, we tested whether human myeloid DC maturation was TLR2 and TLR4 dependent. BCG PGN-mediated DC maturation was blocked by 70% by suppression of both TLR2 and TLR4 and by 30 to 40% by suppression of either of these TLRs. Similar but less profound suppression of BCG CWS-mediated DC maturation was observed. Hence, the presence of BCG PGN is a minimal requirement for activation of both TLR2 and TLR4 in human DCs, unlike the presence of PGNs of gram-positive bacteria, which activate only TLR2. Unexpectedly, however, BCG PGN, unlike BCG CWS, barely activated NF-kappa B in HEK293 cells coexpressing TLR2 plus TLR1, TLR2 plus TLR4, TLR2 plus TLR6, or TLR2 plus TLR10, suggesting that PGN receptors other than TLR2 and TLR4 present on human DCs but not on HEK293 cells are involved in TLR signaling for DC activation.
Structure−Activity Studies of Cerulenin Analogues as Protein Palmitoylation Inhibitors (1999)

Activation of ras oncogenes occurs in a high percentage of tumors, making the enzymes involved in the posttranslational processing of their encoded proteins (p21s) attractive targets for the development of new drugs. Although most effort has focused on farnesyl transferase, which catalyzes the first processing step, attachment of palmitate to p21 is required for optimal transformation by H-ras and N-ras. We have demonstrated that the natural product cerulenin ([2R,3S]-2,3-epoxy-4-oxo-7,10-trans,trans-dodecadienamide) inhibits the palmitoylation of H-ras- and N-ras-encoded p21s in parallel with inhibition of cell proliferation. More than 30 analogues of cerulenin, both aromatic and aliphatic, with various chain lengths and amide substitutions, have been synthesized for use in SAR studies. Studies on the inhibition of T24 cell proliferation indicate that the alpha-keto-epoxy moiety is critical for cytotoxicity, while alkyl chain length had only modest effects on potency. Several compounds inhibited the incorporation of [(3)H]palmitate into p21 in intact T24 cells, with the unsubstituted carboxamides being more active than N,N-dimethyl compounds. In contrast to the effects on palmitoylation, the only compounds which inhibited fatty acid synthase contained alkyl side chains of 12 carbons or fewer. Regression analyses indicated that inhibition of palmitoylation is more closely related to inhibition of proliferation than is inhibition of fatty acid synthase. Further characterization of the molecular pharmacology of these and analogous compounds may define a new class of drugs with antitumor activity.
Cost-Based Pricing of Individual Automobile Risk Transfer: Car-Mile Exposure Unit Analysis (1993)

Every mile traveled by a car transfers risk to its insurer. This paper posits that the product of a cents-per-mile rate based on class experience and the miles recorded on the car’s odometer appropriately earns prepaid premium while the car is driven. Operation of a practical car-mile system is described briefly. To test the competing idea that driver-record pricing responds to known large differences in risk transfer, a model used to validate claim free discounts is reexamined with the car-mile as the measure of individual cost. Driver-record pricing is found to inflate car-year price-to-cost differences. Consequences of accident rate variability for a car-mile system are reviewed. The per mile cost of individual risk transfer is a class property because of the random nature of accidents. Driver-record pricing attempted on a per mile basis would amplify differences within classes. Key words and phrases: Per mile insumnce, nccidenf rate, risk clnssificotion, driver record nzodel, nwrit rating 1
Phase I trial of recombinant adenovirus gene transfer in lung cancer. Longitudinal study of the immune responses to transgene and viral products. (1997)

Animal studies indicate that the use of replication-deficient adenovirus for human gene therapy is limited by host antivector immune responses that result in transient recombinant protein expression and blocking of gene transfer when rechallenged. Therefore, we have examined immune responses to an adenoviral vector and to the beta-galactosidase protein in four patients with lung cancer given a single intratumor injection of 10(9) plaque-forming units of recombinant adenovirus. The beta-galactosidase protein was expressed in day-8 tumor biopsies from all patients at variable levels. Recombinant virus DNA was detected by PCR in day-30 and day-60 tumor biopsies from all patients except patient 1. A high level of neutralizing antiadenovirus antibodies was detected in patient 1 before Ad-beta-gal injection whereas it was low (patient 3) or undetectable in the other two patients. All patients developed potent CD4 type 1 helper T cell (Th1) responses to adenoviral particles which increased gradually over time after injection. Antiadenovirus cytotoxic T lymphocyte responses were consistently boosted in the two patients examined (patients 3 and 4). Sustained production of anti-beta-galactosidase IgG was observed in all patients except patient 1. Consistent with anti-beta-gal antibody production, all patients except patient 1 developed intense, dose-dependent Th1 responses to soluble beta-galactosidase which increased over time. Strong beta-galactosidase-specific cytotoxic T lymphocyte responses were detected in patients 2, 3, and 4. Our results clearly show that despite the intensity of antiadenovirus responses, transgene protein expression was sufficient to induce strong and prolonged immunity in three patients. Recombinant adenovirus injected directly into the tumor is a highly efficient vector for immunizing patients against the transgene protein.
A New Entry to Asymmetric Platinum(IV) Complexes via Oxidative Chlorination (2014)

Pt(IV) complexes are usually prepared by oxidation of the corresponding Pt(II) counterparts, typically using hydrogen peroxide or chlorine. A different way to synthesize asymmetrical Pt(IV) compounds is the oxidative chlorination of Pt(II) counterparts with N-chlorosuccinimide. The reaction between cisplatin cis-[PtCl2(NH3)2], carboplatin, cis-[PtCl2(dach)] and cis-[Pt(cbdc)(dach)] (cbdc = cyclobutane-1,1'-dicarboxylato; dach = cyclohexane-1R,2R-diamine) with N-chlorosuccinimide in ethane-1,2-diol was optimized to produce the asymmetric Pt(IV) octahedral complexes [PtA2Cl(glyc)X2] (A2 = 2 NH3 or dach; glyc = 2-hydroxyethanolato; X2 = 2 Cl or cbdc) in high yield and purity. The X-ray crystal structure of the [Pt(cbdc)Cl(dach)(glyc)] complex is also reported. Moreover, the oxidation method proved to be versatile enough to produce other mixed Pt(IV) derivatives varying the reaction medium. The two trichlorido complexes easily undergo a pH-dependent hydrolysis reaction, whereas the dicarboxylato compounds are stable enough to allow further coupling reactions for drug targeting and delivery via the glyc reactive pendant. Therefore, the coupling reaction between the [Pt(cbdc)Cl(dach)(glyc)] and a model carboxylic acid, a model amine, and selectively protected amino acids is reported.
Integration of pharmacokinetic and pharmacodynamic studies in the discovery, development, and review of protein therapeutic agents: A conference report (2001)

Clinical Pharmacology &amp; Therapeutics (2001) 69 , 387–399; doi: 10.1067/mcp.2001.115455
An artificial lateral line system using IPMC sensor arrays (2012)

Most fish and aquatic amphibians use the lateral line system, consisting of arrays of hair-like neuromasts, as an important sensory organ for prey/predator detection, communication, and navigation. In this paper a novel bio-inspired artificial lateral line system is proposed for underwater robots and vehicles by exploiting the inherent sensing capability of ionic polymer–metal composites (IPMCs). Analogous to its biological counterpart, the IPMC-based lateral line processes the sensor signals through a neural network. The effectiveness of the proposed lateral line is validated experimentally in the localization of a dipole source (vibrating sphere) underwater. In particular, as a proof of concept, a prototype with body length (BL) of 10 cm, comprising six millimeter-scale IPMC sensors, is constructed and tested. Experimental results have shown that the IPMC-based lateral line can localize the source from 1–2 BLs away, with a maximum localization error of 0.3 cm, when the data for training the neural network are collected from a grid of 2 cm by 2 cm lattices. The effect of the number of sensors on the localization accuracy has also been examined.
CCK-8 potentiates the insulinotropic action of glucagon-like-peptide I (1989)

Journal Article CCK-8 potentiates the insulinotropic action of glucagon-like-peptide I Get access H C Fehmann, H C Fehmann Zentrum Innere Medizin, Universität Marburg Search for other works by this author on: Oxford Academic Google Scholar R Göke, R Göke Zentrum Innere Medizin, Universität Marburg Search for other works by this author on: Oxford Academic Google Scholar B Göke, B Göke Zentrum Innere Medizin, Universität Marburg Search for other works by this author on: Oxford Academic Google Scholar G Richter, G Richter Zentrum Innere Medizin, Universität Marburg Search for other works by this author on: Oxford Academic Google Scholar M Trautmann, M Trautmann Zentrum Innere Medizin, Universität Marburg Search for other works by this author on: Oxford Academic Google Scholar R Arnold R Arnold Zentrum Innere Medizin, Universität Marburg Search for other works by this author on: Oxford Academic Google Scholar Acta Endocrinologica (Norway), Volume 120, Issue 3_Supplement, Jun 1989, Page S17, https://doi.org/10.1530/acta.0.120S017 Published: 01 June 1989