gebhart/patrepeval huggingface dataset:
Cytotoxic CD4 and CD8 T lymphocytes, generated by mutant p21‐ras (12VAL) peptide vaccination of a patient, recognize 12VAL‐dependent nested epitopes present within the vaccine peptide and kill autologous tumour cells carrying this mutation (1997)

Mutant p21-ras proteins contain sequences that distinguish them from normal ras, and represent unique epitopes for T-cell recognition of antigen-bearing tumour cells. Here, we examined the capacity of CD4+ and CD8+ T cells, generated simultaneously by mutant-ras-peptide vaccination of a pancreatic-adenocarcinoma patient, to recognize and lyse autologous tumour cells harbouring corresponding activated K-ras epitopes. The patient was vaccinated with a purified 17mer ras peptide (KLVVVGAVGVGKSALTI), containing the Gly12 → Val substitution. Responding T cells were cloned following peptide stimulation, and CD4+ and CD8+ peptide-specific cytotoxic T lymphocytes(CTL) were obtained. Transient pancreatic-adenocarcinoma cell lines(CPE) were established in cell culture from malignant ascites of the patient, and were shown to harbour the same K-ras mutation as found in the primary tumour. These cells were efficiently killed by the T-cell clones and CD8+-mediated cytotoxicity was HLA-class-I-restricted, as demonstrated by inhibition of lysis by anti-class-I monoclonal antibodies. By employing as targets different class-I-matched tumour cell lines expressing a 12Val mutation, we were able to demonstrate HLA-B35 as the restriction molecule, and further use of peptide-sensitized EBV-B cells as target cells identified VVVGAVGVG as the nonamer peptide responsible for CD8+-T-cell recognition. These data demonstrate that peptide vaccination with a single mutant p21-ras-derived peptide induces CD4+ and CD8+ CTL specific for nested epitopes, including the Gly → Val substitution at codon 12, and that both these T-cell sub-sets specifically recognize tumour cells harbouring the corresponding K-ras mutation. Int. J. Cancer 72:784–790, 1997. © 1997 Wiley-Liss, Inc.
Computer Modelling the Reclaim Action of a Self-unloading Vessel (1993)

In recent years there has been a steady increase in the number of self unloading ships involved in the world shipping trade. This paper focuses on unloading difficulties encountered on board the M.V. River Yarra, a vessel converted to self-unloading configuration in late 1991 which is now working around the Australian coastline. These difficulties arose when discharging cohesive materials such as gypsum, salt and slag. Due to the design of the reclaimer units a portion of the cargo within each hold was not positively reclaimed. This resulted in large unstable sections of cargo which caused damage to the reclaimer unit when they collapsed. The investigation involved the development of computer based solid models of each hold, the determination of strength and frictional characteristics of the various cargoes and a review of the slope stability of the cargo within the hold. From the solids model, the amount of cargo positively reclaimed and the capacity lost due to alterations to the holds were calculated. Measurement of the frictional and bulk strength characteristics allowed an analysis of the product stability within the hold during reclaim.
A 0 dB-IL, 2140±30 MHz bandpass filter utilizing Q-enhanced spiral inductors in standard CMOS (2002)

A 3/sup rd/-order Chebyshev bandpass filter, that employs on-chip passive elements with Q-enhancement technique, achieves an insertion loss of 0 dB and a passband of 60 MHz around a center frequency of 2140 MHz. Fabricated in a 0.25-/spl mu/m CMOS, the filter operates with 2.5-V supply and 7 mA. The filter occupies an area of 1.3 mm /spl times/2.7 mm.
Glutamine Utilization by the Small Intestine (1982)

This chapter contains sections titled: Introduction Utilization of Arterial Glutamine Utilization of Luminal Glutamine Enzymology Physiologic Implications Concluding Remarks
Surface Fixation of Hydrogels (1992)

The authors report a new photochemical technique for the fixation of hydrogels onto the surface of fabricated devices and sensors. The preparative method is based on two different types of photochemistry: photocross-linking due to intermolecular photodimerization of cinnamoyl groups, and photoinduced covalent bonding due to the photoreactivity of azido groups. The immobilization of heparin, useful as a thromboresistant coating, onto poly(ethylene terephthalate), and the immobilization of glucose oxidase onto the surface of a carbon fiber electrode, were demonstrated. In the former, immobilized heparin was found to be continuously released from the hydrogel, and the release rate was effectively controlled by the thickness of the hydrogel. In the latter, upon the addition of glucose, a spontaneous electrical response was observed to function as a glucose sensor. No delamination was found for either system after vigorous washing with water. Thus, durable surface hydrogelation, simultaneous immobilization of bioactive substances, and functioning of the systems were demonstrated.
Borna disease virus in mice: host-specific differences in disease expression (1993)

We developed a mouse model of Borna disease to facilitate immunopathogenesis research by adaptation of Borna disease virus to mice through serial passage in mouse brain tissue. Borna disease virus replication, antibody production, inflammation, and Borna disease expression in several different strains of mice were examined.
Increased Expression of System xc− in Glioblastoma Confers an Altered Metabolic State and Temozolomide Resistance (2016)

SLC7A11, in addition to redox modulation, appears to be associated with increased cellular metabolism and is a mediator of temozolomide resistance in human glioma, thus making system x<sub>C</sub><sup>-</sup> a potential therapeutic target in glioblastoma multiforme. Mol Cancer Res; 14(12); 1229-42. ©2016 AACR.
Phase II trial of high‐dose, intermittent calcitriol (1,25 dihydroxyvitamin D3) and dexamethasone in androgen‐independent prostate cancer (2006)

The response rate reported in the current study (19%) was not found to be clearly higher than expected with dexamethasone alone. High-dose intermittent calcitriol plus dexamethasone appears to be safe, feasible, and has antitumor activity.
Three-Dimensional nand Flash Architecture Design Based on Single-Crystalline STacked ARray (2011)

Various critical issues related with 3-D stacked nand Flash memory are examined in this paper. Our single-crystalline STacked ARray (STAR) has many advantages such as better scalability, possibility of single-crystal channel, less sensitivity to 3-D interference, stable virtual source/drain characteristic, and more extendability over other stacked structures. With STAR, we proposed a unit 3-D structure, i.e., “building.” Then, using this new component, 3-D block and full chip architecture are successfully designed. For the first time, the structure and operation methods of the “full” array are considered. The fully designed 3-D nand Flash architecture will be the novel solution of reliable 3-D stacked nand Flash memory for terabit density.
Effect of Hysterectomy vs Medical Treatment on Health-Related Quality of Life and Sexual Functioning (2004)

Among women with abnormal uterine bleeding and dissatisfaction with medroxyprogesterone, hysterectomy was superior to expanded medical treatment for improving health-related quality-of-life after 6 months. With longer follow-up, half the women randomized to medicine elected to undergo hysterectomy, with similar and lasting quality-of-life improvements; those who continued medical treatment also reported some improvements.
SYNTHESE D'INDOLE PHOSPHONATES PAR CYCLISATION SELON FISCHER D'ARYLHYDRAZONES PHOSPHONATES (1988)

Abstract La cyclisation selon Fischer d'arylhydrazones d'oxo-1,2 et 3 alkylphosphonates permet d'acctderi à divers indole phosphonates 1–5. Les acides phosphoniques correspondants 1′-5′ ont Cgalement été préparés. Les structures des différents indoles sont confirmées par spectroscopie RMN31P, 1H et 13C. The Fischer cyclization of 1,2,3-oxoalkylphosphonates arylhydrazones into indole phosphonates 1–5 is described. The corresponding phosphonic acids 1′-5′ are also prepared. The structures of these indoles have been determined by 31P, 1H and 13CNMR spectroscopy.
Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti (1998)

The mariner transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito, Aedes aegypti. To determine if this demonstrated mobility could be utilized to genetically transform the mosquito, a modified mariner element marked with a wild-type allele of the Drosophila melanogaster cinnabar gene was microinjected into embryos of a kynurenine hydroxylase-deficient, white-eyed recipient strain. Three of 69 fertile male founders resulting from the microinjected embryos produced families with colored-eyed progeny individuals, a transformation rate of 4%. The transgene-mediated complementation of eye color was observed to segregate in a Mendelian manner, although one insertion segregates with the recessive allele (female-determining) of the sex-determining locus, and a separate insertion is homozygous lethal. Molecular analysis of selected transformed families demonstrated that a single complete copy of the construct had integrated independently in each case and that it had done so in a transposase-mediated manner. The availability of a mariner transformation system greatly enhances our ability to study and manipulate this important vector species.
Can a Selective PPARγ Modulator Improve Glycemic Control in Patients With Type 2 Diabetes With Fewer Side Effects Compared With Pioglitazone? (2014)

INT131 demonstrated dose-dependent reductions in HbA1c, equivalent to 45 mg pioglitazone, but with less fluid accumulation and weight gain, consistent with its SPPARM design.
Effective jump-pointer prefetching for linked data structures (1999)

Current techniques for prefetching linked data structures (LDS) exploit the work available in one loop iteration or recursive call to overlap pointer chasing latency. Jump pointers, which provide direct access to non-adjacent nodes, can be used for prefetching when loop and recursive procedure bodies are small and do not have sufficient work to overlap a long latency. This paper describes a framework for jump-pointer prefetching (JPP) that supports four prefetching idioms: queue, full, chain, and root jumping and three implementations: software-only, hardware-only, and a cooperative software/hardware technique. On a suite of pointer intensive programs, jump pointer prefetching reduces memory stall time by 72% for software, 83% for cooperative and 55% for hardware, producing speedups of 15%, 20% and 22% respectively.
访问妈妈：我从哪里来？——教学“生殖与发育”的创意作业及思考 (2014)

一说起作业，人们头脑中就会出现一张张试卷，一道道练习题，甚至一个个要记忆的概念，于是，“过重的课业负担”成为阻碍学生身心健康发展的羁绊。能不能改变作业的面貌，把传统的试卷、训练题、机械抄写变成一个轻松愉快、主动参与的过程，让学生感到作业不枯燥，甚至很好玩，在游戏中学习，在参与中学习，巩固所学知识，还能激发学生的学习兴趣，拓展学生视野，锻炼学生的综合能力？本刊撷取一组泰山学院附属中学孙明霞老师的创意作业以飨读者，或许你能从中找到答案。
K‐252b Selectively Potentiates Cellular Actions and trk Tyrosine Phosphorylation Mediated by Neurotrophin‐3 (1992)

K-252b, a protein kinase inhibitor, has been shown earlier to inhibit nerve growth factor actions on cholinergic neurons of the basal forebrain. In the present study, K-252b was found to prevent trophic actions of two other neurotrophins, brain-derived neurotrophic factor, and neurotrophin-3, on central cholinergic and dopaminergic neurons, peripheral sensory neurons, and PC12 pheochromocytoma cells, when used at greater than 2 microM concentration. Comparable actions of nonneurotrophin growth factors were not affected. Surprisingly, at 0.1-100 nM, K-252b selectively enhanced the trophic action of neurotrophin-3 on central cholinergic neurons, peripheral sensory neurons, and PC12 cells. In PC12 cells, K-252b potentiated the neurotrophin-3-induced tyrosine phosphorylation of trk, a protein kinase responsible for transmitting neurotrophin signals. Of the three structurally related nerve growth factor inhibitors, K-252a, K-252b, and staurosporine, only the first two also mediated neurotrophin-3 potentiation. These findings indicate that K-252b generally and selectively potentiates the neurotrophic action of neurotrophin-3 and suggest that this action involves trk-type neurotrophin receptors.
Protein Engineering Strategies for Sustained Glucagon-Like Peptide-1 Receptor–Dependent Control of Glucose Homeostasis (2008)

Taken together, our data demonstrate that CNTO736 produces a spectrum of GLP-1 receptor-dependent actions while exhibiting significantly improved pharmacokinetics relative to the native GLP-1 peptide.
Oridonin stabilizes retinoic acid receptor alpha through ROS-activated NF-κB signaling (2015)

Oridonin stabilizes RARα protein by increasing cellular ROS levels, which causes activation of the NF-κB signaling pathway.
Abstract #267: PEGPH20: PEGylated recombinant human hyaluronidase antitumor activity in the 4T1 orthotopic breast carcinoma model (2009)

Hyaluronan (HA) is a significant component of the extracellular matrix (ECM) in many solid tumors. In breast carcinoma, accumulation of HA is associated with tumor progression and poor prognosis. Using the 4T1-GFP murine breast carcinoma line as a model, we probed the effects of HA depletion enzymatically using a pegylated recombinant human PH20 hyaluronidase (PEGPH20). When grown as a monolayer, 4T1-GFP cells assembled large hyaluronan dependent pericellular matrices that were sensitive to a 60 minute exposure with 1,000U/mL (~30µg/mL) PEGPH20. Treatment of 4T1-GFP/3T3 hybrid spheroid multicellular aggregates with PEGPH20 (10,000 U/mL) resulted in a 10% decrease in colony diameter and a 26% reduction in colony volume as early as 1 hour after incubation. Exposure to PEGPH20 in doses ranging from 10-10,000 U/mL reduced 4T1-GFP colony number and inhibited cell proliferation by >50%. In vivo, 4T1-GFP tumors grown orthotopically in mice produced abundant HA in the ECM immediately adjacent to tumor cells. Intravenous (IV) administration of PEGPH20 with doses up to 500K U/kg depleted HA from the ECM of 4T1-GFP tumors and was well tolerated, with a dose-dependent decrease in HA labeling within 3 days of administration. Depletion of HA staining was observed following treatment with PEGPH20 doses > 50K U/kg (~1.6 mg/kg). Anti-tumor activity of PEGPH20 was evaluated using repeat (Q2D) dosing (150K - 1500K U/kg) in 4T1-GFP orthotopic tumor bearing mice, with 150K U/kg effectively removing all ECM HA. Approximately 60% tumor growth inhibition (TGI) was observed when measured 3 days after the last PEGPH20 dose. Furthermore the time for 4T1-GFP tumors to reach ~1,500 mm3 volume was also delayed by >1.7-fold, compared to control mice. Survival to 1500 mm3 tumor volume in the PEGPH20 (150K U/kg) treated group was statistically significantly different (p Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 267.
Inhibition of human muscle sympathetic activity by sensory stimulation (2002)

Surprising sensory stimuli causing arousal are known to evoke short-lasting activation of human sympathetic activity in skin but not in muscle nerves; anecdotal observations suggest that there may even be an inhibition of muscle sympathetic nerve activity (MSNA). To test this hypothesis we recorded multiunit MSNA in the peroneal nerve in 19 subjects aged 19-71 years, while sensory stimuli, consisting of either an electrical skin stimulus to a finger or a visual flash, were delivered repeatedly with intervals of approximately 20 s. The stimuli were given either 200 or 400 ms after the R wave of the electrocardiogram. Dummy stimuli, consisting of trigger pulses without sensory stimulation served as controls. Electrical skin resistance reductions were monitored from the palm of a hand as electrodermal signs of arousal-induced cutaneous sympathetic activity. On a group basis both types of sensory stimuli attenuated the amplitude of one or two bursts of MSNA, while no such effects occurred after dummy stimuli. Individually, the inhibition was evoked by at least one stimulus modality or delay in 16 subjects whereas in three subjects no significant inhibition occurred. Skin resistance responses were evoked in all subjects. Some subjects responded to one, others to both stimulus modalities, and electrical stimuli were more effective than visual stimuli in causing MSNA inhibition as well as skin resistance reduction. On the other hand, electrodermal signs of arousal were equally common in subjects with and without inhibitory responses. We suggest that the MSNA inhibition evoked by sensory stimuli is an arousal effect which varies markedly between individuals.
GaInNAsSb/GaAs Photodiodes for Long-Wavelength Applications (2011)

GaInNAsSb p-i-n photodetectors on GaAs substrates capable of detecting wavelengths up to 1550 nm with a reduced dark current are presented in this letter. Responsivities of 0.18 A/W at 1300 nm and 0.098 A/W at 1550 nm were achieved in devices with a ~0.5-μm-thick GaInNAsSb p-i-n epitaxial layer with 10% In, 4.08% N, and 4.4% Sb. The absorption coefficient (α) spectra show that α is intrinsically higher than that of the indirect-gap Ge layer but ~2.6 times lower than that reported for a In <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.53</sub> Ga <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.47</sub> As epitaxial layer at 1550 nm. The dark currents of the GaInNAsSb devices are found to be lower than not only those of the GaInNAs devices of a similar energy gap but also the state-of-the-art Ge/Si avalanche photodiodes. The lower dark currents in the GaInNAsSb devices compared with the GaInNAs devices can possibly be attributed to the reduction of defects in the Sb-containing epitaxial layer.
Baculovirus Expression Vectors for Insect and Mammalian Cells (2007)

Functional expression of recombinant proteins has become a routine, but critical tool in modern molecular biology. Since their introduction, the use of baculovirus vectors to produce proteins for purification has become one of the most widely-used viral gene delivery systems as expression levels obtained are difficult to match with any other eukaryotic expression system. Extensive engineering to simplify and accelerate the process of recombinant virus construction has made this system accessible to virtually any modern biological laboratory. The utility of baculoviruses has been broadened with the discovery that appropriately modified virus can mediate gene expression in a wide variety of mammalian cell lines, and thus can function as a flexible cell-based assay development tool. The wide range of applications and potential for commercialization of products leads to consideration of a number of aspects of the system.
Photolithographic Technique for Direct Photochemical Modification and Chemical Micropatterning of Surfaces (2004)

We describe a photolithographic method for the direct modification and micropatterning of the surface chemical structure of self-assembled monolayers. End-functional azobenzene alkanethiols are designed and synthesized so that, when self-assembled onto gold substrates, an acid-sensitive tert-butyl ester end group is positioned at the air−monolayer interface. Upon exposure to UV radiation in the presence of a photoacid generator, the tert-butyl ester groups are removed in the form of butylene gas to form surface carboxylic acid groups. The orientation of the monolayers and photochemical surface modification reactions are characterized by X-ray photoelectron spectroscopy measurements. The photochemical change from hydrophobic tert-butyl ester groups to hydrophilic carboxylic acid groups causes a significant change in wettability reflected in a water contact angle change from 89 to 28°, respectively. Surface chemical modifications may be patterned on a microscale by simply irradiating the self-assembled monolayers in the presence of a photoacid generator through a photomask. Chemically micropatterned surfaces are shown to be effective templates for patterned surface assembly of amine-terminated polystyrene colloidal particles and fluorescently tagged polystyrene nanoparticles and for the spatial patterning of water droplets. The photochemical changes that occur are constrained structurally to occur only at the air−monolayer surface and require only deep UV radiation and a photoacid generator as reagents. Direct photochemical micropatterning by this method is a simple and direct process that retains the full spatial resolution of deep UV photolithography and can be carried out with conventional photomasking equipment.
Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views (2007)

Mesenchymal stem cells or multipotent stromal cells (MSCs) isolated from the bone marrow of adult organisms were initially characterized as plastic adherent, fibroblastoid cells with the capacity to generate heterotopic osseous tissue when transplanted in vivo. In recent years, MSCs or MSC-like cells have been shown to reside within the connective tissue of most organs, and their surface phenotype has been well described. A large number of reports have also indicated that the cells possess the capacity to transdifferentiate into epithelial cells and lineages derived from the neuroectoderm. The broad developmental plasticity of MSCs was originally thought to contribute to their demonstrated efficacy in a wide variety of experimental animal models of disease as well as in human clinical trials. However, new findings suggest that the ability of MSCs to alter the tissue microenvironment via secretion of soluble factors may contribute more significantly than their capacity for transdifferentiation in tissue repair. Herein, we critically evaluate the literature describing the plasticity of MSCs and offer insight into how the molecular and functional heterogeneity of this cell population, which reflects the complexity of marrow stroma as an organ system, may confound interpretation of their transdifferentiation potential. Additionally, we argue that this heterogeneity also provides a basis for the broad therapeutic efficacy of MSCs.
Ex vivo diffusion tensor imaging and quantitative tractography of the rat spinal cord during long‐term recovery from moderate spinal contusion (2008)

Results from the current study support the hypothesis that the spinal cord undergoes continual changes during recovery from SCI.
Two-photon molecular spectroscopy (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTwo-photon molecular spectroscopyW. Martin McClainCite this: Acc. Chem. Res. 1974, 7, 5, 129–135Publication Date (Print):May 1, 1974Publication History Published online1 May 2002Published inissue 1 May 1974https://pubs.acs.org/doi/10.1021/ar50077a001https://doi.org/10.1021/ar50077a001research-articleACS PublicationsRequest reuse permissionsArticle Views1255Altmetric-Citations261LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts