gebhart/patrepeval huggingface dataset:
Selective Plasma Hydrolysis of Glucocorticoid γ-Lactones and Cyclic Carbonates by the Enzyme Paraoxonase: An Ideal Plasma Inactivation Mechanism (1999)

ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTSelective Plasma Hydrolysis of Glucocorticoid γ-Lactones and Cyclic Carbonates by the Enzyme Paraoxonase: An Ideal Plasma Inactivation MechanismKeith Biggadike, Richard M. Angell, Colin M. Burgess, Rosanne M. Farrell, Ashley P. Hancock, Andy J. Harker, Wendy R. Irving, Chris Ioannou, Panayiotis A. Procopiou, Rupert E. Shaw, Yemisi E. Solanke, Onkar M. P. Singh, Michael A. Snowden, Rob J. Stubbs, Sarah Walton, and Helen E. WestonView Author Information Medicinal Chemistry, Enzyme Pharmacology, Biomet Discovery and Protein Science Units, Glaxo Wellcome Research and Development, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, U.K. Cite this: J. Med. Chem. 2000, 43, 1, 19–21Publication Date (Web):December 11, 1999Publication History Received25 August 1999Published online11 December 1999Published inissue 1 January 2000https://pubs.acs.org/doi/10.1021/jm990436thttps://doi.org/10.1021/jm990436trapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1373Altmetric-Citations160LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Genetics,Lactones,Molecular structure,Peptides and proteins,Plasma Get e-Alerts
Detection of DNA Double-strand Breaks in Synchronous Cultures of CHO Cells by Means of Asymmetric Field Inversion Gel Electrophoresis (1991)

A pulsed field gel electrophoresis technique, asymmetric field inversion gel electrophoresis (AFIGE), was used to evaluate induction by X-rays of DNA damage in CHO cells. The fraction of DNA activity released from the plug (FAR) was used as a measure for the amount of radiation-induced DNA damage, predominantly DNA double-strand breaks (dsb) (Stamato and Denko 1990), and was determined at various stages of growth and phases of the cell cycle in a range of doses between zero and 70 Gy. The FAR per unit dose fluctuated throughout the cell cycle and was high for cells irradiated in G1; it decreased as cells entered S and reached a minimum in the middle of this phase. The FAR per unit dose increased again as cells progressed towards the end of S, and reached values in G2 similar to those measured in G1. When damage was introduced into DNA by means of 125I decay similar fluctuations in the FAR per decay were observed throughout the cell cycle, suggesting that the variations in the FAR per unit of radiation dose observed throughout the cell cycle do not derive from alterations in the induction of dsb. The fluctuations in the FAR per unit dose throughout the cell cycle were quantitatively similar to the fluctuations in the fraction of activity eluted in irradiated cells assayed by the non-unwinding filter elution assay throughout the cycle (Okayasu et al. 1988), and suggest that both techniques respond to similar DNA replication-associated alterations of the biophysical and/or biochemical properties of the DNA molecule. It is concluded that caution needs to be exercised before differences observed in the FAR between different cell lines or between various phases of the cell cycle after exposure to a given dose of radiation are interpreted as suggesting differences in the induction of DNA dsb.
Cambio de piel: Literature and Evil (1989)

BSS Subject Index: CAMBIO DE PIEL [C. FUENTES]EVIL/DEVIL [AS LITERARY/CULTURAL THEME]FUENTES, CARLOS (b. 1928)
Application of Lattice Boltzmann Method for Simulation of Turbulent Diffusion from a CO_2 Lake in Deep Ocean (2012)

This paper presents a numerical method based on the Lattice Boltzmann (LB) scheme, which aims to accurately model and simulate the carbon dioxide (CO_2) dissolution, diffusion, and convection process in the condition of complex ocean current, turbulence and varying topography of the ocean floor. This LBM based scheme is carefully validated by using several benchmark test cases. A two-dimensional simulation study of CO_2 dissipation from a CO_2 lake is carried out to demonstrate the capability of the proposed method. The influence of the ocean current turbulence on the CO_2 dissolution rate is also ciscussed.
Regulation in free‐radical polymerization of vinyl acetate and synthesis of end‐group modified poly(vinyl alcohol)s (1993)

Abstract A kinetical investigation on the consumption of a chain transfer agent in the free‐radical polymerization of vinyl acetate (VAc) was carried out. A new method to control the degree of polymerization of poly(vinyl acetate) (PVAc) or poly(vinyl alcohol) (PVA) as well as the end group content by continuous addition of a chain transfer agent, whose chain transfer constant is much larger than unity such as thiol compounds, during the course of VAc polymerization was proposed. The method using 2‐mercaptoethanol was applied to the preparation of lowmolecular‐weight PVAc and PVA with M̄ w / M̄ n ≈ 2.
Individualism to Collaboration: The significance of teacher culture to the induction of newly qualified teachers (2001)

This paper draws upon qualitative data collected as part of a study of the implementation of new arrangements for the monitoring and support of newly qualified teachers (NQTs) in England (DfEE, 1999). Interview data from NQTs, induction tutors and head-teachers are used to demonstrate the significance of teacher culture to the experience of teachers during their first year in post. We locate the cultures within which NQTs work on a continuum that we characterise as moving from highly individualised, through structural collaboration to spontaneously collaborative. The new arrangements appear to have made the greatest impact on schools where the culture is one of structural collaboration. Individualised cultures are problematic for NQTs, while spontaneously collaborative cultures provide a highly supportive and developmental environment independently of the mandatory arrangements.
Rabbit very low density lipoprotein receptor: a low density lipoprotein receptor-like protein with distinct ligand specificity. (1992)

A cDNA that expresses a receptor for very low density lipoprotein (VLDL) was isolated from a rabbit heart cDNA library and characterized. The deduced amino acid sequence of the cDNA revealed that the cDNA encodes a protein with striking homology to the low density lipoprotein (LDL) receptor. Like the LDL receptor, the mature protein consists of the following five domains spanning 846 amino acids: 328 N-terminal amino acids including an 8-fold repeat of 40 amino acids homologous to the ligand binding repeat of the LDL receptor; 396 amino acid residues homologous to the epidermal growth factor precursor including three cysteine-rich repeats; a region immediately outside of the plasma membrane rich in serines and threonines; 22 amino acids traversing the plasma membrane; and 54 amino acids including the NPVY sequence that is required for clustering of the LDL receptor in coated pits and that projects into the cytoplasm. LDL-receptor-deficient Chinese hamster ovary cells transfected with the cDNA bound and internalized VLDL, beta-migrating VLDL, and intermediate density lipoprotein but did not bind LDL with high affinity. The 3.6- and 9.5-kilobase mRNAs for the VLDL receptor are highly abundant in heart, muscle, and adipose tissue. Barely detectable amounts of the mRNAs were present in liver. Based on the structural features, ligand specificity, and tissue expression of the mRNAs, we suggest that this VLDL receptor may mediate uptake of apolipoprotein E-containing lipoproteins enriched with triglyceride in nonhepatic tissues that are active in fatty acid metabolism.
Nucleotide sequences of the Pseudomonas savastanoi indoleacetic acid genes show homology with Agrobacterium tumefaciens T-DNA (1985)

We report the nucleotide sequences of iaaM and iaaH, the genetic determinants for, respectively, tryptophan 2-monooxygenase and indoleacetamide hydrolase, the enzymes that catalyze the conversion of L-tryptophan to indoleacetic acid in the tumor-forming bacterium Pseudomonas syringae pv. savastanoi. The sequence analysis indicates that the iaaM locus contains an open reading frame encoding 557 amino acids that would comprise a protein with a molecular weight of 61,783; the iaaH locus contains an open reading frame of 455 amino acids that would comprise a protein with a molecular weight of 48,515. Significant amino acid sequence homology was found between the predicted sequence of the tryptophan monooxygenase of P. savastanoi and the deduced product of the T-DNA tms-1 gene of the octopine-type plasmid pTiA6NC from Agrobacterium tumefaciens. Strong homology was found in the 25 amino acid sequence in the putative FAD-binding region of tryptophan monooxygenase. Homology was also found in the amino acid sequences representing the central regions of the putative products of iaaH and tms-2 T-DNA. The results suggest a strong similarity in the pathways for indoleacetic acid synthesis encoded by genes in P. savastanoi and in A. tumefaciens T-DNA.
Generation of Recombinant Vaccinia Viruses (1998)

This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid-transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.
Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis (1999)

The specificity for 11-deoxycortisol (11-DOC) of a monoclonal antibody (mAb), designated SCET, was changed to specificity for cortisol (CS) by site-specific mutagenesis followed by random mutagenesis. The Fab form of SCET was expressed on the surface of a phage. During the first step, mutations were introduced at 14 amino acid positions in three complementarity-determining regions (CDRs) of the VH domain that seemed likely to form the steroid-binding pocket. A clone, DcC16, was isolated from the resultant library with multiple mutations and this clone was shown to have CS-binding activity but also to retain high 11-DOC-binding activity. During the second step, mutations were introduced randomly into the entire VH-coding region of the DcC16 clone by an error-prone polymerase chain reaction, and CS-specific mutant antibodies were selected in the presence of 11-DOC as a competitor. Three representative clones were analyzed with the BIAcore instrument, and each revealed a large increase in the binding constant for CS and a decrease in that for 11-DOC. Structural models, constructed by computer simulation, indicated the probable molecular basis for these changes in specificity.
Screening for bacterial vaginosis: a novel application of artificial nose technology. (1997)

The AromaScan system was used to analyse vaginal swabs from 68 women attending a genitourinary clinic. Using clinical criteria, subjects were assessed for bacterial vaginosis. After training the AromaScan system to recognise patterns generated from four patients with and four patients without bacterial vaginosis, 16 of the 17 (94%) remaining subjects were correctly identified as having the condition. The positive predictive value of the test was 61.5%. These results indicate that the AromaScan technology may be of value as a screening test for bacterial vaginosis.
Immobilized RGD peptides on surface-grafted dextran promote biospecific cell attachment (2001)

Dextran has recently been investigated as an alternative to poly(ethylene glycol) (PEG) for low protein-binding, cell-resistant coatings on biomaterial surfaces. Although antifouling properties of surface-grafted dextran and PEG are quite similar, surface-bound dextran has multiple reactive sites for high-density surface immobilization of biologically active molecules. We recently reported nontoxic aqueous methods to covalently immobilize dextran on material surfaces. These dextran coatings effectively limited cell adhesion and spreading in the presence of serum-borne cell adhesion proteins. In this study we utilized the same nontoxic aqueous methods to graft cell adhesion peptides on low protein-binding dextran monolayer surfaces. Chemical composition of all modified surfaces was verified by X-ray photoelectron spectroscopy (XPS). Surface-grafted cell adhesion peptides stimulated endothelial cell, fibroblast, and smooth muscle cell attachment and spreading in vitro. In contrast, surface-grafted inactive peptide sequences did not promote high levels of cell interaction. Surface-grafted high affinity cyclic RGD peptides promoted cell type-dependent interactions. With dextran-based surface coatings, it will be possible to develop well-defined surface modifications that promote specific cell interactions and perhaps better performance in long-term biomaterial implants.
Interaction at end-plate receptors between different choline derivatives (1957)

Abstract Interaction between different choline derivatives has been studied by applying them simultaneously to a motor end-plate and recording the resulting changes in the membrane potential of the muscle fibre. Choline potentiates the depolarizing effect of acetylcholine (Ach) when applied in normal Ringer. Decamethonium has a ‘diphasic’ action, initial depression of the Ach effect being followed by more prolonged potentiation. When these experiments are made after treating the muscle with an esterase inhibitor (prostigmine 10-6 w/v), the potentiation of the Ach effect, by decamethonium or choline, is absent and replaced by simple ‘curare-like’ inhibition. When decamethonium is allowed to interact with a rapidly acting stable ester (carbaminoylcholine or succinylcholine), it produces simple 'curare-like’ inhibition. The triple effects of choline and decamethonium, i. e. (i) weak depolarization, (ii) potentiation of Ach in normal Ringer solution, (iii) inhibition of Ach in the presence of prostigmine, can be explained by competitive reactions between the drugs and receptor as well as Ach-esterase molecules. It is suggested that the first step in a depolarizing end-plate reaction is the formation of an intermediate, inactive, compound between drug and receptor.
Partial deletion of the long arm of chromosome 16 and bone marrow eosinophilia in acute nonlymphocytic leukemia: a new association (1983)

Recently, several specific chromosomal abnormalities have been associated with distinctive clinical and/or morphological subtypes of acute nonlymphocytic leukemia (ANLL). To further investigate the clinical utility of karyotype analysis in ANLL, we have examined G-banded metaphase chromosomes at diagnosis in 61 consecutive patients. Of the 60 patients who had adequate mitoses, 47 (78%) had a clonal chromosome abnormality. The sole karyotypic abnormality found in 5 patients was a del(16)(q22). The unique pretreatment characteristic of these 5 patients was marrow eosinophilia ranging from 8% to 54%. No other patient had more than 4% marrow eosinophils. Among the patients with eosinophilia, all had Auer rods, serum muramidase was elevated in the 4 tested, and 4 had hepatomegaly at presentation. Both patients who survived initial treatment remain in complete remission at 23+ and 33+ mo. The data suggest that we have identified a new cytogenetic-clinical subtype of ANLL defined by the del(16)(q22).
Automated phase-measuring profilometry of 3-D diffuse objects (1984)

The high resolution surface profile of a 3-D diffuse object is obtained by measurement of the phase distribution across the image of a projected sinusoidal grating deformed by the surface. A shearing polarization interferometer is used for projection. Deformed grating images are detected by an array camera and processed by a microcomputer. Surface height resolutions of better than 10 μm have been attained, limited essentially by electronic quantization noise. In contrast to direct deformed grating analysis, this method, based on phase-shifting interferometric techniques, is capable of accurate measurement even with coarse projected gratings and low density image sensing arrays. Areas of application include industrial quality control, biostereometrics, robotics, and solid modeling for computer graphics.
2-Phenylindole-linked [2-(aminoalkyl)pyridine]dichloroplatinum(II): complexes with a selective action on estrogen receptor positive mammary tumors (1991)

A number of [2-(aminomethyl)pyridine]dichloroplatinum(II) complexes, linked to 5-hydroxy-2-(4-hydroxyphenyl)indoles by alkyl spacer groups of varying lengths, were synthesized and studied for their binding affinities for the calf uterine estrogen receptor. Their relative binding affinity (RBA) values ranged from 1.0 to 5.2% (estradiol, RBA = 100%). Highest affinities were found with complexes possessing a (CH2)5- or (CH2)6-bridge between the pyridine aminomethyl group and the indole nitrogen. Endocrine activities of the complexes and their ligands, determined in the mouse uterine weight test, were low. All compounds entered comparative tests using estrogen receptor positive and negative mammary tumor models. In cell culture, a growth inhibiting effect was only observed in hormone-sensitive MCF-7 cells, but not in hormone-independent MDA-MB 231 cells. In this assay, there was no significant difference between complexes and their ligands. In vivo, the growth of estrogen receptor positive MXT mouse mammary tumors was strongly inhibited by the complexes whereas the hormone-independent MXT mammary tumors showed only a minor response. At a dose of 3 X 20 mg/kg per week, complexes 10d-g reduced the tumor weight by ca. 80% after 6 weeks of treatment. This effect was generally stronger than that exerted by the ligands. The doses applied were well tolerated. Since the complexes described in this paper require the estrogen receptor for their action, a mechanism similar to that of antiestrogens is assumed.
Prerenal Failure: A Deleterious Shift from Renal Compensation to Decompensation (1988)

TRADITIONALLY, the term "prerenal failure" was used to describe a clinical situation in which the structurally intact kidney fails as a result of an impairment in glomerular perfusion. The decreased delivery of blood to the glomerulus was thought to precipitate a fall in the glomerular filtration rate, with subsequent development of azotemia. Our current understanding of this syndrome, however, is much more complicated than this earlier, simplistic formulation, in which renal function was viewed as being generally subservient to alterations in the systemic circulatory status, particularly cardiac output. In addition to the retention of salt and water, induced by adaptations . . .
Supercoiling of the DNA template during transcription. (1987)

Transcription of a right-handed double-helical DNA requires a relative rotation of the RNA polymerase and its nascent RNA around the DNA. We describe conditions under which the resistance to the rotational motion of the transcription ensemble around the DNA can be large. In such cases, the advancing polymerase generates positive supercoils in the DNA template ahead of it and negative supercoils behind it. Mutual annihilation of the positively and negatively supercoiled regions may be prevented by anchoring points on the DNA to a large structure, or, in the case of an unanchored plasmid, by the presence of two oppositely oriented transcription units. In prokaryotes, DNA topoisomerase I preferentially removes negative supercoils and DNA gyrase (topoisomerase II) removes positive ones. Our model thus provides an explanation for the experimentally observed high degree of negative or positive supercoiling of intracellular pBR322 DNA when DNA topoisomerase I or gyrase is respectively inhibited. We discuss the implications of our model in terms of supercoiling regulation, DNA conformational transitions, and gene regulation in both prokaryotes and eukaryotes.
PERP expression stabilizes active p53 via modulation of p53-MDM2 interaction in uveal melanoma cells (2011)

The activation and regulation of target genes by the tumour-suppressor p53 dictates the fate of a cell, with cell cycle arrest or apoptosis being two distinct outcomes. PERP (p53 apoptosis effector related to PMP-22), a p53 transcriptional target, is induced specifically during apoptosis but not cell cycle arrest. Downregulation of PERP is associated with the aggressive, monosomy 3-type of uveal melanoma (UM), the most common primary intraocular tumour in adults, and increased PERP expression has a pro-apoptotic effect in UM cells. Here, we identify a novel effect of PERP expression, as elevated PERP protein positively influences active levels of its own transcriptional regulator, p53. Using fluorescent fusion proteins of PERP, p53 and MDM2, we demonstrate in single living UM cells that PERP expression significantly enhances p53 activity and its nuclear localization, increases p53-dependent transcription (including that of MDM2) while allowing oscillatory nucleo-cytoplasmic shuttling of p53/MDM2 complexes. Phosphorylation of p53 serine residues that interfere with the interaction between p53 and its negative regulator MDM2 and enhance pro-apoptotic gene transcription also occurs subsequent to PERP expression. These results implicate a role for PERP in amplifying functional p53 levels that promote p53-dependent apoptosis, and reveal a potential target for exploitation in enhancing p53 activity.
Immediate replacement of a maxillary central incisor associated with severe facial bone loss: use of Bio-Oss collagen--case report. (2003)

The loss and replacement of anterior maxillary teeth pose several challenges. Treatment must successfully address immediate tooth replacement, esthetics, patient comfort, and psychologic acceptance. The purpose of this case report is to present a prosthetic and surgical technique for immediate tooth replacement following extraction in an area of severe localized bone loss. Combined full- and partial-thickness flaps associated with the use of Bio-Oss collagen grafting material and a Bio-Gide membrane were used for alveolar ridge reconstruction. To improve the final esthetic result, a connective tissue graft was used to increase ridge volume and papillary height.