gebhart/patrepeval huggingface dataset:
Folate Conjugated Fluorescent Silica Nanoparticles for Labeling Neoplastic Cells (2005)

We describe a novel technique of using fluorescent silica nanoparticles (FSNPs) to detect over-expressed folate receptors, as typical for certain malignancies (metastatic adenocarcinoma, pituitary adenoma and others). Using Stöber's method with some modification, 135 nm size FSNPs were synthesized by a hydrolysis and co-condensation reaction of tetraethylorthosilicate (TEOS), fluorescein labeled (3-aminopropyl)triethoxysilane (APTS) and a water-dispersible silane reagent, (3-trihydroxysilyl)propyl methylphosphonate (THPMP) in the presence of ammonium hydroxide catalyst. Folic acid (folate) was covalently attached to the amine modified FSNPs by a carbodiimide coupling reaction. The characterization of folate-FSNPs was performed using a variety of spectroscopic (UV-VIS and fluorescence), microscopic (transmission electron microscopy, TEM) and light scattering techniques. Folate conjugated FSNPs were then targeted to human squamous cancer cells (SCC-9). Laser scanning confocal images successfully demonstrated the labeling of SCC-9 cells and the efficacy of FSNP based detection system.
Albumin transfer across the choroid plexus of South American opossum (Monodelphis domestica). (1997)

1. Blood-cerebrospinal fluid (CSF) transfer of various exogenous albumins has been investigated in developing Monodelphis domestica (South American grey short-tailed opossum) and compared with the steady-state CSF: plasma ratios for endogenous (Monodelphis) albumin. Ratios for Monodelphis albumin and human albumin were similar and were the highest at postnatal day 5 (P5) (48.2 +/- 4.4 and 40.6 +/- 4.5%, respectively). The ratio for bovine albumin was similar to the steady-state ratio for Monodelphis albumin at P7-8 but became consistently lower than the Monodelphis albumin ratio at all other ages until P32-36 when all albumins tested attained a similar low ratio. The CSF:plasma ratio of chemically modified (succinylated) bovine albumin was always significantly lower than that of other albumins, except at the oldest age examined (P32-36). 2. Immunocytochemistry showed that within the brain, albumin was confined to the lumen and endothelial cells of blood vessels. In the choroid plexus only a small proportion (0.2-1.7% of the total cell number) of epithelial cells was positive for albumin, both endogenous and exogenous, at all ages studied (except the 3rd ventricle where cells were only positive from P8). The CSF was strongly positive for all albumins. The peak proportion of positive cells and of albumin concentrations in CSF occurred at P8. These findings suggest that the primary route for penetration of albumin into CSF is directly across the choroid plexus rather than via the brain. 3. Double-labelling immunocytochemistry revealed that the same epithelial cells contained both endogenous (Monodelphis) and exogenous (human) albumin. In contrast, for succinylated albumin, at P7 only about 35% (lateral ventricle) and 50% (4th ventricle) of Monodelphis albumin-positive cells were also positive for succinylated albumin, but by P30 this proportion increased to 90% at both sites. 4. Thus the developing choroid plexus distinguishes between different albumins. Chemical modification of albumin (succinylation) disrupts this mechanism. It is proposed that in older animals (P32-36) all of the albumin in the CSF is derived from plasma by diffusion (as in adult animals). At earlier stages of development, a proportion of the albumin in CSF also appears to be transferred from the plasma by diffusion with an additional component transferred by a mechanism that can distinguish between different species of albumin. The main route of entry of albumin to CSF seems likely to be via the choroid plexus epithelial cells.
Hypomagnesemia and diabetes mellitus. A review of clinical implications. (1996)

Hypomagnesemia has long been known to be associated with diabetes mellitus. Mather et al confirmed the presence of hypomagnesemia in nearly 25% of their diabetic out-patients. Low serum magnesium level has been reported in children with insulin-dependent diabetes and through the entire spectrum of adult type I and type II diabetics regardless of the type of therapy. Hypomagnesemia has been correlated with both poor diabetic control and insulin resistance in nondiabetic elderly patients.
Efficacy of human chorionic gonadotrophin and gonadotrophin releasing hormone for hastening ovulation in Thoroughbred mares (1986)

Plasma progesterone levels were measured daily to determine the accuracy of diagnosing ovulation by rectal palpation carried out every other day; 81.5 per cent mares injected with human chorionic gonadotrophin showed increases of progesterone more than 1 ng/ml by 72 h after injection compared with 65 per cent of mares injected with gonadotrophin releasing hormone (GnRH) or saline. Mating at ovulation achieved a 74 per cent pregnancy rate in mares given hCG compared with 50 per cent given GnRH and 45 per cent controls. Diagnosis of ovulation per rectum on the basis of a pit in the ovarian surface or the presence of a soft friable structure was found to be accurate in 91 per cent of cases. Diagnosis based on the presence of a firm plum-like structure or the disappearance of a previously identified follicle at a given site was 60 per cent accurate. In cases of rectal diagnosis of non-ovulation 38 per cent had actually ovulated based on a rise in progesterone more than 1 ng/ml and a further 19 per cent showed a rise in progesterone by 24 h after the diagnosis. The use of hCG may improve conception rates by synchronisation of ovulation and mating and where rectal palpation of the ovaries remains the only basis for timed mating.
Mice Lacking the Neuropeptide α-Calcitonin Gene-Related Peptide Are Protected Against Diet-Induced Obesity (2010)

Alpha-calcitonin gene-related peptide (alphaCGRP) is a neuropeptide that is expressed in motor and sensory neurons. It is a powerful vasodilator and has been implicated in diverse metabolic roles. However, its precise physiological function remains unclear. In this study, we investigated the role of alphaCGRP in lipid metabolism by chronically challenging alphaCGRP-specific knockout (alphaCGRP(-/-)) and control mice with high-fat diet regimens. At the start of the study, both animal groups displayed similar body weights, serum lipid markers, and insulin sensitivity. However, alphaCGRP(-/-) mice displayed higher core temperatures, increased energy expenditures, and a relative daytime (nonactive) depression in respiratory quotients, which indicated increased beta-oxidation. In response to fat feeding, alphaCGRP(-/-) mice were comparatively protected against diet-induced obesity with an attenuated body weight gain and an overall reduction in adiposity across all the three diets examined. AlphaCGRP(-/-) mice also displayed improved glucose handling and insulin sensitivity, lower im and hepatic lipid accumulation, and improved overall metabolic health. These findings define a new role for alphaCGRP as a mediator of energy metabolism and opens up therapeutic opportunities to target CGRP action in obesity.
The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium (2000)

Salmonella typhimurium is an invasive pathogen that causes diseases ranging from mild gastroenteritis to enteric fever. During the infection process, S. typhimurium induces a number of virulence genes required to circumvent host defences and/or acquire nutrients in the host. We have used the in vivo expression technology (IVET) system to select for S. typhimurium genes that are induced after invasion of a murine cultured cell line. We have characterized a putative iron transporter in Salmonella pathogenicity island 1, termed sitABCD. The sitABCD operon is induced under iron-deficient conditions in vitro and is repressed by Fur. This locus is induced in the animal specifically after invasion of the intestinal epithelium. We show that a sit null mutant is significantly attenuated in BALB/c mice, suggesting that SitABCD plays an important role in iron acquisition in the animal.
High-speed polygon-scanner-based wavelength-swept laser source in the telescope-less configurations with application in optical coherence tomography (2008)

A compact high-speed tuning laser source is demonstrated in two different configurations using a polygonal mirror scanner without a telescope. It is shown that the filter configuration finesse increases by utilizing multiple reflections from the polygon facet(s) and grating illumination(s). Theoretically, the free spectral range (FSR), the instantaneous linewidth, and the finesse of each filter configuration are derived. For single grating illumination, the measured coherence length, FSR, and power were 2.8 mm, 184 nm, and 40 mW at the scanning frequency of 50 kHz, respectively. Coherence length, FSR, and power of the second laser configuration were 6.2 mm, 117 nm, and 35 mW, respectively. Finally, images of a human finger were acquired in vivo using two proposed swept-source configurations.
Glucan receptor and zymosan-induced lysosomal enzyme secretion in macrophages (1995)

A receptor for beta-glucan was in the present study shown to mediate binding of zymosan particles to resident mouse peritoneal macrophages. Lysosomal enzyme secretion in response to zymosan was maximal at a low particle/cell ratio, continuous for at least 3 h after particle/cell contact and inhibitable by soluble glucan. Latex particles of various size caused no selective secretory response, but at high particle/cell ratios were toxic. By use of a fluorescent ligand, the macrophage beta-glucan receptor was shown to be trypsin-sensitive, Ca2+/Mg(2+)-independent, recirculating and also present in an intracellular mobilizable pool. Binding of ligand to the beta-glucan receptor and inhibition of the lysosomal secretory response to zymosan were both more efficient with glucans of larger size, indicating that clustering of glucan receptors at the cell surface occurs. Such clustering could stabilize ligand binding by multiple interactions and possibly trigger intracellular signaling events on binding of zymosan particles.
Synthesis of an N-Alkyl Derivative of 2'-Deoxyisoguanosine (1997)

3',5'-O-bis-tert-butyldimethylsilyl-2'-deoxyguanosine is converted in two steps to 3',5'-O-tert-butyldimethylsilyl-6-O-aryl-2'-deoxyxanthosine. This compound is used to make a 2'-deoxyisoguanosine analog with a functionalized side chain.
Nutch: A Flexible and Scalable Open-Source Web Search Engine (2005)

Nutch is an open-source Web search engine that can be used at global, local, and even personal scale. Its initial design goal was to enable a transparent alternative for global Web search in the public interest — one of its signature features is the ability to “explain ” its result rankings. Recent work has emphasized how it can also be used for intranets; by local communities with richer data models, such as the Creative Commons metadata-enabled search for licensed content; on a personal scale to index a user&amp;apos;s files, email, and web-surfing history; and we also report on several other research projects built on Nutch. In this paper, we present how the architecture of the Nutch system enables it to be more flexible and scalable than other comparable systems today.
Mechanical Design and Finite Element Analysis of Tunable Fabry-Perot MEMS Structures for Adaptive Infrared Detectors (2005)

Tunable Fabry-Perot (FP) filters have been recently used in MEMS devices, such as adaptive infrared detectors. The mechanical characteristics of these filters are significantly influenced by the mechanical design of the structure, which eventually affects the filter performance and device integrity. This paper reports the mechanical design and optimization of FP filter structures by means of finite element modeling
Binding of lipoprotein lipase to heparin. Identification of five critical residues in two distinct segments of the amino-terminal domain. (1993)

Binding to heparan sulfate governs many aspects of the physiological action and regulation of the lipolytic enzyme, lipoprotein lipase (LPL). In an attempt to identify the structural determinants which mediate this interaction, basic residues in three segments of the primary sequence of human LPL (residues 147-151, 279-282, and 292-304) were replaced with alanine, either singly or in various combinations, and variant proteins were subjected to affinity chromatography on heparin-Superose. Five basic residues in two distinct segments of the primary sequence were critical determinants of the high affinity for heparin manifested by the active enzyme (R279, K280, R282, K296, R297). By contrast, no such evidence could be detected for basic residues in the first cluster (K147, K148) or for other basic residues in the third cluster (K292, R294, K304), while the evidence for K300 was unresolved. The conformation of this heparin-binding domain can be inferred by reference to the three-dimensional structure of the homologous enzyme, pancreatic lipase (Winkler, F. K., D'Arcy, A., and Hunziker, W. (1990) Nature 343, 771-774). Affinity of the active enzyme for heparin could not be reduced below a threshold, suggesting that other heparin-binding determinants exist elsewhere in the molecule, as supported by recently published evidence (Davis, R. C., Wong, H., Nikazy, J., Wang, K., Han, Q., and Schotz, M. C. (1992) J. Biol. Chem. 267, 21499-21504).
Analysis of 13C and 15N CPMAS NMR‐spectra of soil organic matter and composts (1991)

15N and 13C CPMAS spectra of composted plants are presented. The plants (L. rigidium and Zea mays) were grown in 15N enriched medium and fermented for several months until an approx. 80% of the dry matter was lost. In all 15N spectra the secondary amide/peptide peaks at 87 ppm contributes more than 80% of the total intensity. No new 15N peaks are formed during the fermentation process. Older attempts to assign a significant fraction of humic acid nitrogen to heteroaromatic structures formed in the fermentation process are thus most probably wrong.
Total Synthesis of (+-)-17β-Hydroxy-Δ9(10)-des-A-androsten-5-one [(+-)-2,3,3a,4,5,7,8,9,9aβ,9bα-Decahydro-3β-hydroxy-3aβ6-dimethyl-1H-benz[e]inden-7-one] (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal Synthesis of (+-)-17β-Hydroxy-Δ9(10)-des-A-androsten-5-one [(+-)-2,3,3a,4,5,7,8,9,9aβ,9bα-Decahydro-3β-hydroxy-3aβ6-dimethyl-1H-benz[e]inden-7-one]Zoltan Hajos, Robert Micheli, David Parrish, and Eugene OlivetoCite this: J. Org. Chem. 1967, 32, 10, 3008–3010Publication Date (Print):October 1, 1967Publication History Published online12 August 2003Published inissue 1 October 1967https://pubs.acs.org/doi/10.1021/jo01285a601https://doi.org/10.1021/jo01285a601research-articleACS PublicationsRequest reuse permissionsArticle Views783Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cesarean Section and Chronic Immune Disorders (2014)

Cesarean delivery exemplifies a shared environmental risk factor in early life associating with several chronic immune diseases. Understanding commonalities in the underlying mechanisms behind chronic diseases may give novel insight into their origin and allow prevention.
The structure of 2Zn pig insulin crystals at 1.5 Å resolution (1988)

The paper describes the arrangement of the atoms within rhombohedral crystals of 2Zn pig insulin as seen in electron density maps calculated from X-ray data extending to 1.5 A (1 A = 10(-10) m = 10(-1) nm) at room temperature and refined to R = 0.153. The unit cell contains 2 zinc ions, 6 insulin molecules and about 3 x 283 water molecules. The atoms in the protein molecules appear well defined, 7 of the 102 side chains in the asymmetric unit have been assigned alternative disordered positions. The electron density over the water molecules has been interpreted in terms of 349 sites, 217 weighted 1.0, 126 weighted 0.5, 5 at 0.33 and 1 at 0.25 giving ca. 282 molecules. The positions and contacts of all the residues belonging to the two A and B chains of the asymmetric unit are shown first and then details of their arrangement in the two insulin molecules, 1 and 2, which are different. The formation from these molecules of a compact dimer and the further aggregation of three dimers to form a hexamer around two zinc ions, follows. It appears that in the packing of the hexamers in the crystal there are conflicting influences; too-close contacts between histidine B5 residues in neighbouring hexamers are probably responsible for movements of atoms at the beginning of the A chain of one of the two molecules of the dimer that initiate movements in other parts, particularly near the end of the B chain. At every stage of the building of the protein structure, residues to chains of definite conformation, molecules, dimers, hexamers and crystals, we can trace the effect of the packing of like groups to like, aliphatic groups together, aromatic groups together, hydrogen-bonded structures, positive and negative ions. Between the protein molecules, the water is distributed in cavities and channels that are continuous throughout the crystals. More than half the water molecules appear directly hydrogen bonded to protein atoms. These are generally in contact with other water molecules in chains and rings of increasing disorder, corresponding with their movement through the crystals. Within the established crystal structure we survey next the distribution of hydrogen bonds within the protein molecules and between water and protein and water and water; all but eight of the active atoms in the protein form at least one hydrogen bond.(ABSTRACT TRUNCATED AT 400 WORDS)
Cystic Fibrosis Heterozygote Resistance to Cholera Toxin in the Cystic Fibrosis Mouse Model (1994)

The effect of the number of cystic fibrosis (CF) alleles on cholera toxin (CT)-induced intestinal secretion was examined in the CF mouse model. CF mice that expressed no CF transmembrane conductance regulator (CFTR) protein did not secrete fluid in response to CT. Heterozygotes expressed 50 percent of the normal amount of CFTR protein in the intestinal epithelium and secreted 50 percent of the normal fluid and chloride ion in intestinal epithelium and secreted 50 percent of the normal fluid and chloride ion and fluid secretion suggests that CF heterozygotes might possess a selective advantage of resistance to cholera.
Effects of dexamethasone on proliferation, differentiation and apoptosis of adult human osteoblasts in vitro. (2003)

Dexamethasone has a significant effect on the proliferation and differentiation of adult osteoblasts in vitro without apoptosis, and dexamethasone at the suggested concentration can be used as positive control in drug studies for osteoporosis treatment.
Sensor-based road model estimation for autonomous driving (2017)

In the course of the development and integration of the autonomous driving the knowledge about the current environment and especially the road is one of the basic requirements to fulfill the automated driving task. This information is often extracted from a precise map provided in the vehicle. Therefore the road course and the individual lanes are known in advance by using the map and a suitable ego position estimation. However, in some situations such a map may be invalid and therefore unusable. This can be a driving area which was never mapped or regions where the map is outdated because the environment has changed. The following paper addresses the problem of building a road model without using a map by only fusing measurements from different sensors mounted on the ego vehicle. As sensor measurements various information like lane markings painted on the ground, the position of other cars or occupancy grids can be used. They are transformed into a grid-based model and a geometrical description is extracted out of this model by the use of a novel path-planning based method. The proposed approach was tested with a vehicle equipped with sensors and real measurement data from German highways.
Some practical strategies for reducing intermodulation in satellite communications (1988)

Various heuristic procedures for obtaining practical solutions to the general one-level carrier frequency assignment problem are described. The problem treated is general in the sense that it accommodates the case where L of the N slots may be explicitly designated as prohibited and unavailable for assignment. This problem occurs in satellite transmission with many small carriers accessing the same transponder where, due to multipath and TV interference from crosspolarized transponders of the same satellite and from copolarized transponders of the adjacent satellites, some portions of the bandwidth of the considered transponder cannot be used. To permit comparison with respect to the intermodulation advantage and central-processing-unit time required, the case without prohibited slots is considered. The sequential insertion procedure in which, starting with two carriers at the two end slots, one additional carrier at a time is optimally inserted into one of the unassigned slots is found best when the ratio between the available bandwidth and the total carrier bandwidth is greater than about 125%. All the heuristic procedures produced assignments whose intermodulation advantages are all greater than the bandwidth ratio.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Direct Solid-Phase Hydrogenation of Fullerenes (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDirect Solid-Phase Hydrogenation of FullerenesChangming Jin, Robert Hettich, Robert Compton, David Joyce, James Blencoe, and Timothy BurchCite this: J. Phys. Chem. 1994, 98, 16, 4215–4217Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://doi.org/10.1021/j100067a004RIGHTS & PERMISSIONSArticle Views293Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (403 KB) Get e-Alerts