gebhart/patrepeval huggingface dataset:
Pressure-Programmed Supercritical Fluid Chromatography of Wide Molecular Weight Range Mixtures (1970)

A versatile, high resolution supercritical fluid chromatograph with a pressure programming option is described. It is, in essence, the liquid chromatograph described earlier, (1) which has been modified to allow for the use of a supercritical fluid as the moving phase. The pressure programmer enables wide molecular weight range compounds to be processed in short times. The ability to adjust the pressure also allows many separations to be carried out under optimum operating conditions. The performance of this unit is demonstrated by its ability to separate mixtures of polynuclear aromatic hydrocarbons in times one magnitude shorter than necessary in liquid chromatography, to fractionate a commercially available “monodisperse” polystyrene of up to a molecular weight of several thousands into its separate oligomers, and to resolve some isomers in a 538 average molecular weight polyphenyl ether sample.
The expression of saporin, a ribosome-inactivating protein from the plant Saponaria officinalis, in Escherichia coli. (1993)

We have isolated and sequenced genomic clones from the DNA of Saponaria officinalis using a cDNA probe that encodes proteins with high homology to saporin-6, one of the most potent of the ribosome-inactivating proteins that is currently used for the construction of immunotoxins and mitotoxins. Sequence differences in the clones suggest a multigene family of proteins. These data agree with observations of several different proteins with ribosome-inactivating protein activity and similar structure. Two of the genomic clones encode proteins that have identical sequences to two of the four isoforms of saporin-6. We have inserted the DNA from one genomic clone into an Escherichia coli expression system that encodes a signal sequence for export to the bacterial periplasmic space. Exportation is observed and the isolated gene product has ribosome-inactivation activity similar to the native protein. Sequence analysis shows differential processing of the remaining plant signal sequence. The majority of the expressed protein remains intracellular and this material also shows high specific activity and differential processing. Saporin as an immunotoxin in clinical trial and as a mitotoxin in experimental models has been extremely efficacious. These data indicate the ability to produce fusion proteins with saporin and cell-binding ligands for production of new reagents for further clinical and experimental use.
Toll-like receptors and T-helper-1/T-helper-2 responses (2003)

A greater understanding of the processes by which TLRs regulate adaptive immunity may yield not only improved ways to treat infectious diseases but also new approaches to the treatment and prevention of allergic and certain autoimmune disorders.
Separate Cl- conductances activated by cAMP and Ca2+ in Cl(-)-secreting epithelial cells. (1990)

We studied the cAMP- and Ca2(+)-activated secretory Cl- conductances in the Cl(-)-secreting colonic epithelial cell line T84 using the whole-cell patch-clamp technique. Cl- and K+ currents were measured under voltage clamp. Forskolin or cAMP increased Cl- current 2-15 times with no change in K+ current. The current-voltage relation for cAMP-activated Cl- current was linear from -100 to +100 mV and showed no time-dependent changes in current during voltage pulses. Ca2+ ionophores or increased pipette Ca2+ increased both Cl- and K+ currents 2-30 times. The Ca2(+)-activated Cl- current was outwardly rectified, activated during depolarizing voltage pulses, and inactivated during hyperpolarizing voltage pulses. Addition of ionophore after forskolin further increased Cl- conductance 1.5-5 times, and the current took on the time-dependent characteristics of that stimulated by Ca2+. Thus, cAMP and Ca2+ activate Cl- conductances with different properties, implying that these second messengers activate different Cl- channels or that they induce different conductive and kinetic states in the same Cl- channel.
A highly sensitive method for mapping the 5′ termini of mRNAs (1993)

Journal Article A highly sensitive method for mapping the 5′ termini of mRNAs Get access Micheline Fromont-Racine, Micheline Fromont-Racine Institut Jacques Monod du CNRS, Université Paris7, Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Edouard Bertrand, Edouard Bertrand Institut Jacques Monod du CNRS, Université Paris7, Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Raymond Pictet, Raymond Pictet Institut Jacques Monod du CNRS, Université Paris7, Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Thierry Grange Thierry Grange * Institut Jacques Monod du CNRS, Université Paris7, Tour 43, 2 Place Jussieu, 75251 Paris Cedex 05, France * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 21, Issue 7, 11 April 1993, Pages 1683–1684, https://doi.org/10.1093/nar/21.7.1683 Published: 11 April 1993 Article history Received: 05 January 1993 Revision received: 21 February 1993 Accepted: 21 February 1993 Published: 11 April 1993
Abstract 2933: Pre-clinical model of eradication of B cell leukemia with lentiviral transduced anti-CD19 chimeric immunoreceptor-modified T cells (2010)

Abstract CD19 is a membrane glycoprotein found on human B lymphocytes at all stages of maturation, and is not lost during malignant transformation to precursor-B cell Acute Lymphoblastic Leukemia (ALL). T cells engineered with chimeric immunoreceptors (CIR) consisting of an extracellular scFv domain against CD19 fused to the T cell receptor ζ signal transduction domain represent a promising adoptive immunotherapy against leukemias refractory to traditional chemotherapy. Previous studies from our group and others have demonstrated that inclusion of signaling domains from costimulatory molecules such as CD28 or CD137 (41BB) can improve the antileukemic efficacy and in vivo persistence of CIR-modified T cells. To critically evaluate the anti-tumor efficacy of anti-CD19-targeting CIR that contained various signaling domains alone or in combination we utilized the highly immunodeficient NOG mouse model and an aggressive CD19+ leukemia cell line (Nalm-6) and treated with primary human CD8+ and CD4+ lymphocytes gene modified to express anti-CD19 CIR with TcR zeta alone, CD28-TcRzeta, CD137-TcRzeta, and CD28- CD137-TcR signalling domains. NOG mice were injected with 1×106 Nalm-6 on Day 1, a dose that produces 100% engraftment in the bone marrow by Day 7 (95% CI 0.15-1.2% of total bone marrow cells CD19+) with 100% mortality by Day 25 if untreated. T cells were expanded ex vivo using anti-CD3/CD28 beads and subjected to lentiviral gene transfer of various CIRs, and 1×107 T cells were injected on Day 7 (7×106 CAR +). Mice were followed weekly by quantitative flow cytometric analysis of peripheral blood for CD3, CD4, CD8 and CD19 positive human cells. A separate cohort of animals were treated with either Nalm-6 or T cells transduced with firefly luciferase to track biodistribution and treatment response. Mice treated with T cells engineered to express each of the anti-CD19 CAR demonstarted statistically significant improved survival over control animals, with animals succumbing either to leukemia, or in a construct dependent manner, GVHD. Additionally, a hierarchy of survival advantage was observed among the anti-CD19 constructs, with the CD19-CD28-CD137-ζ CAR mediating most enhanced survival, with 37% of those mice surviving until the end of the study at Day 125. In vivo bioluminescent imaging demonstrated that T cell migration and expansion depends on antigen recognition and presence of the ζ chain in the CIR, and that leukemia clearance is unexpectedly rapid (&amp;lt; 3 days). These data indicate it is possible to eradicate established bone marrow disease with a single injection of lentiviral transduced CIR T cells. Future efforts will focus on leukemia rechallenge to evaluate in vivo persistence and surveillance capabilities of these CIR T cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2933.
Photoinduced electron transfer in supramolecular systems for artificial photosynthesis (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotoinduced electron transfer in supramolecular systems for artificial photosynthesisMichael R. WasielewskiCite this: Chem. Rev. 1992, 92, 3, 435–461Publication Date (Print):May 1, 1992Publication History Published online1 May 2002Published inissue 1 May 1992https://pubs.acs.org/doi/10.1021/cr00011a005https://doi.org/10.1021/cr00011a005research-articleACS PublicationsRequest reuse permissionsArticle Views13727Altmetric-Citations2779LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Theory of Characteristic Modes Revisited: A Contribution to the Design of Antennas for Modern Applications (2007)

The objective of this paper is to summarize the work that has been developed by the authors for the last several years, in order to demonstrate that the Theory of Characteristic Modes can be used to perform a systematic design of different types of antennas. Characteristic modes are real current modes that can be computed numerically for conducting bodies of arbitrary shape. Since characteristic modes form a set of orthogonal functions, they can be used to expand the total current on the surface of the body. However, this paper shows that what makes characteristic modes really attractive for antenna design is the physical insight they bring into the radiating phenomena taking place in the antenna. The resonance frequency of modes, as well as their radiating behavior, can be determined from the information provided by the eigenvalues associated with the characteristic modes. Moreover, by studying the current distribution of modes, an optimum feeding arrangement can be found in order to obtain the desired radiating behavior.
Notes- β-Aryl-glutaconic Acids. II. Imides of Certain β-Aryl-glutaconic and Glutaric Acids (1959)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNotes- β-Aryl-glutaconic Acids. II. Imides of Certain β-Aryl-glutaconic and Glutaric AcidsJ Nerurkar, R Joshi, and V BhaveCite this: J. Org. Chem. 1959, 24, 12, 2055–2056Publication Date (Print):December 1, 1959Publication History Published online18 July 2003Published inissue 1 December 1959https://pubs.acs.org/doi/10.1021/jo01094a628https://doi.org/10.1021/jo01094a628research-articleACS PublicationsRequest reuse permissionsArticle Views60Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
3,4‐Diaminopyridine is more effective than placebo in a randomized, double‐blind, cross‐over drug study in LEMS (2009)

The purpose of this study was to investigate the clinical and electrophysiological efficacy of 3,4-diaminopyridine (DAP) in patients with Lambert-Eaton myasthenic syndrome (LEMS) in a randomized, double-blind, cross-over drug trial. The diagnosis of LEMS was made based on the combination of fluctuating muscle weakness, diminished or absent reflexes, and more than 60% increment of the compound muscle action potential (CMAP) amplitude after brief exercise or 50-HZ stimulation on a repetitive nerve stimulation (RNS) test. Evaluations were done at baseline, with placebo, and with 3,4-DAP (up to 75-80 mg/day). Assignment of placebo or 3,4-DAP was done in a double-blinded manner. Measurements included subjective symptoms score, objective clinical measurements [LEMS classification, muscle strength score, quantitative myasthenia gravis (QMG) score] and RNS test and single-fiber electromyography (SFEMG). The differences between placebo and baseline values (placebo change) were compared with the differences between 3,4-DAP and baseline or placebo values (DAP change). Seven patients with LEMS (QMG score >9) participated in the study. One patient had major side-effects with 3,4-DAP and withdrew from the study. Statistically significant efficacy was noted with DAP change (N = 13) compared with placebo change (N = 7) according to the subjective symptoms score (P = 0.01), LEMS classification (P < 0.001), muscle strength score (P < 0.006), QMG score (P = 0.02), and CMAP (P = 0.03). For long-term treatment, 2 patients preferred 3,4-DAP, 1 chose guanidine hydrochloride, 1 preferred pyridostigmine, and 2 chose no treatment. A randomized, double-blind, cross-over drug trial of 3,4-DAP showed significant efficacy over placebo in patients with LEMS. As a long-term treatment, however, not all patients preferred this drug.
Assessment of normal and mutant human presenilin function in  Caenorhabditis elegans (1996)

We provide evidence that normal human presenilins can substitute for Caenorhabditis elegans SEL-12 protein in functional assays in vivo. In addition, six familial Alzheimer disease-linked mutant human presenilins were tested and found to have reduced ability to rescue the sel-12 mutant phenotype, suggesting that they have lower than normal presenilin activity. A human presenilin 1 deletion variant that fails to be proteolytically processed and a mutant SEL-12 protein that lacks the C terminus display considerable activity in this assay, suggesting that neither presenilin proteolysis nor the C terminus is absolutely required for normal presenilin function. We also show that sel-12 is expressed in most neural and nonneural cell types in all developmental stages. The reduced activity of mutant presenilins and as yet unknown gain-of-function properties may be a contributing factor in the development of Alzheimer disease.
Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction (2008)

By presenting antigenic peptides on the cell surface, human leukocyte antigen (HLA) class I molecules are critical for immune defense. Their surface density determines, to a large extent, the level of CD8(+) T cell-dependent immune reactions; their loss is a major mechanism of immune escape. Therefore, powerful processes should regulate their surface expression. Here we document the mechanisms used by CD99 to mediate HLA class I modulation. Up-regulation of HLA class I by IFN-gamma requires CD99. In the trans Golgi network (TGN), and up to the cell surface, CD99 and HLA class I are physically associated via their transmembrane domain. CD99 also binds p230/golgin-245, a coiled-coil protein that recycles between the cytosol and buds/vesicles of the TGN and which plays a fundamental role in trafficking transport vesicles. p230/golgin-245 is anchored within TGN membranes via its Golgin-97, RanBP1, IMh1p, P230 (GRIP) domain and the overexpression of which leads to surface and intracellular down-modulation of HLA class I molecules.
Differential expression of the ufo/axl oncogene in human leukemia-lymphoma cell lines. (1996)

The ufo protein (also termed axl) is a member of a new family of receptor tyrosine kinases and is encoded by a transforming gene that was initially isolated from primary human myeloid leukemia cells by DNA-mediated transformation of NIH/3T3 cells. The ligand, Gas6, a protein S-related molecule lacking any known function yet, has recently been identified. We report the expression pattern of ufo mRNA in a panel of 76 human continuous leukemia-lymphoma cell lines. The gene was not expressed in cell lines derived from lymphoid malignancies (n=28), but transcription was seen in 3/11 myeloid, 0/6 monocytic, 9/13 erythroid and 11/18 megakaryocytic cell lines. Several cell lines were treated with phorbol ester leading to significant upregulation of the ufo message in constitutively positive cells. An apparent ufo mRNA overexpression was not found in any of the positive leukemia cell lines, but was identified in the drug-resistant subclones of the cervix carcinoma cell line HeLa. Southern blot analysis of restriction enzyme-digested genomic DNA did not provide evidence for gene amplification, but the HeLa subclones showed banding patterns suggestive of gene rearrangement. Two main ufo mRNA bands of 3.2 and 5.0 kb were identified; no differences in the half-lives (t1/2 = 2.5 h) of these two mRNA species could be identified. In summary, ufo, representing a novel type of receptor tyrosine kinase, is expressed solely in myeloid and erythro-megakaryocytic leukemias but not in lymphoid malignancies. These and previous data suggest an involvement of the ufo receptor tyrosine kinase in normal and malignant myelopoiesis; however, its exact role, if any, and mode of operation in leukemogenesis remains to be determined.
Regulation and Function of the CD3γ DxxxLL Motif: A Binding Site for Adaptor Protein-1 and Adaptor Protein-2 in Vitro (1997)

Several receptors are downregulated by internalization after ligand binding. Regulation of T cell receptor (TCR) expression is an important step in T cell activation, desensitization, and tolerance induction. One way T cells regulate TCR expression is by phosphorylation/dephosphorylation of the TCR subunit clusters of differentiation (CD)3gamma. Thus, phosphorylation of CD3gamma serine 126 (S126) causes a downregulation of the TCR. In this study, we have analyzed the CD3gamma internalization motif in three different systems in parallel: in the context of the complete multimeric TCR; in monomeric CD4/CD3gamma chimeras; and in vitro by binding CD3gamma peptides to clathrin-coated vesicle adaptor proteins (APs). We find that the CD3gamma D127xxxLL131/132 sequence represents one united motif for binding of both AP-1 and AP-2, and that this motif functions as an active sorting motif in monomeric CD4/ CD3gamma molecules independently of S126. An acidic amino acid is required at position 127 and a leucine (L) is required at position 131, whereas the requirements for position 132 are more relaxed. The spacing between aspartic acid 127 (D127) and L131 is crucial for the function of the motif in vivo and for AP binding in vitro. Furthermore, we provide evidence indicating that phosphorylation of CD3gamma S126 in the context of the complete TCR induces a conformational change that exposes the DxxxLL sequence for AP binding. Exposure of the DxxxLL motif causes an increase in the TCR internalization rate and we demonstrate that this leads to an impairment of TCR signaling. On the basis of the present results, we propose the existence of at least three different types of L-based receptor sorting motifs.
Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters (2009)

Reabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to augment current knowledge in renal physiology and pharmacology. We have established and characterized a conditionally immortalized PT epithelial cell line (ciPTEC) obtained by transfecting and subcloning cells exfoliated in the urine of a healthy volunteer. The PT origin of this line has been confirmed morphologically and by the expression of aminopeptidase N, zona occludens 1, aquaporin 1, dipeptidyl peptidase IV and multidrug resistance protein 4 together with alkaline phosphatase activity. ciPTEC assembles in a tight monolayer with limited diffusion of inulin-fluorescein-isothiocyanate. Concentration and time-dependent reabsorption of albumin via endocytosis has been demonstrated, together with sodium-dependent phosphate uptake. The expression and activity of apical efflux transporter p-glycoprotein and of baso-lateral influx transporter organic cation transporter 2 have been shown in ciPTEC. This established human ciPTEC expressing multiple endogenous organic ion transporters mimicking renal reabsorption and excretion represents a powerful tool for future in vitro transport studies in pharmacology and physiology.
Vaccinia Virus A28L Gene Encodes an Essential Protein Component of the Virion Membrane with Intramolecular Disulfide Bonds Formed by the Viral Cytoplasmic Redox Pathway (2004)

We report the initial characterization of the product of the vaccinia virus A28L gene, which is highly conserved in all sequenced poxviruses. Our studies showed that the A28 protein is expressed at late times during the virus replication cycle and is a membrane component of the intracellular mature virion. An N-terminal hydrophobic sequence, present in all poxvirus A28 orthologs, anchors the protein in the virion surface membrane so that most of it is exposed to the cytoplasm. The cytoplasmic domain contains four conserved cysteines, which form two intramolecular disulfide bonds. Disulfide bond formation depended on the expression of three viral proteins, E10, A2.5, and G4, which together comprise a conserved cytoplasmic redox pathway. A28 is the third identified substrate of this pathway; the others are the L1 and F9 proteins. We constructed a conditional-lethal recombinant vaccinia virus with an inducible A28L gene. The recombinant virus was propagated in the presence of inducer but was unable to replicate and spread in its absence. During a single round of an abortive infection in the absence of inducer, the synthesis and processing of viral proteins, assembly of intra- and extracellular virions, and formation of actin tails occurred normally. In another paper (T. Senkevich, B. M. Ward, and B. Moss, J. Virol. 78:2357-2366, 2004), we have demonstrated that virions assembled without A28 cannot carry out a second round of infection because they are defective in cell penetration.