gebhart/patrepeval huggingface dataset:
The impact of relapsing sterile icodextrin-associated peritonitis on peritoneal dialysis outcome. (2003)

The disappointing result of the relapsing culture-negative ICO-associated peritonitis in our patients was the unavoidable switch to HD, due to the inability of the hypertonic glucose solutions to ensure an adequate ultrafiltration. The moral of the story is that the pharmaceutical industry should market products that are more biocompatible and safer for chronic treatments such as PD.
Marine bioinvasion: Concern for ecology and shipping (2002)

Marine bioinvasion - introduction of marine organisms alien to local ecosystem through ship hulls and ballast water - has serious consequences to native biota, fishery and general coastal ecosystem. Over 80% of the world cargo is mobilized transoceanically and over 12 billion tones of ballast water is filled at one part of the ocean and discharged at the other. These ballast waters offer conducive situation for bacteria, viruses, algae, dinoflagellates and a variety of macro-faunal larval/cyst stages to translocate to alien regions, usually along the coasts of the continents. As an example, there are over 18 species of animals and plants documented along the Indian coasts as those that might have got invaded and established. They can cause deleterious effects to local flora and fauna through their toxigenic, proliferative and over-competitive characteristics. This article points out the threats arising out of marine bioinvasion and various technological developments needed to deal with this unavoidable scourge in global shipping transport.
Novel Amphiphilic Architectures by Ring-Opening Metathesis Polymerization of Macromonomers (1997)

In this study the possibilities offered by “living” ring-opening metathesis polymerization (ROMP) are exploited to engineer novel macromolecular architectures. It is indeed shown that amphiphilic branched structures of hitherto unreported topologies can be prepared by ROMP of miscellaneous macromonomers, provided the latter polymers carry an end-standing norbornene unsaturation. Janus-type architectures are, for instance, accessible by sequential ROMP of polystyrene (PS) and poly(ethylene oxide) (PEO) macromonomers; other original branched structures, whose topology makes them particularly attractive for applications such as unimolecular micelles or associative thickeners, can be obtained through homopolymerization of macromonomers based on PS-b-PEO diblock copolymers.
Computer animation with scripts and actors (1982)

A technique and philosophy for controlling computer animation is discussed. Using the Actor/Scriptor Animation System (ASAS) a sequence is described by the animator as a formal written SCRIPT, which is in fact a program in an animation/graphic language. Getting the desired animation is then equivalent to “debugging” the script. Typical images manipulated with ASAS are synthetic, 3D perspective, color, shaded images. However, the animation control techniques are independent of the underlying software and hardware of the display system, so apply to other types (still, B&amp;W, 2D, line drawing ...). Dynamic (and static) graphics are based on a set of geometric object data types and a set of geometric operators on these types. Both sets are extensible. The operators are applied to the objects under the control of modular animated program structures. These structures (called actor s) allow parallelism, independence, and optionally, synchronization, so that they can render the full range of the time sequencing of events. Actor s are the embodiment of imaginary players in a simulated movie. A type of animated number can be used to drive geometric expressions (nested geometrical operators) with dynamic parameters to produce animated objects. Ideas from programming styles used in current Artificial Intelligence research inspired the design of ASAS, which is in fact an extension to the Lisp programming environment. ASAS was developed in an academic research environment and made the transition to the “real world” of commercial motion graphics production.
Genome integrity of myeloproliferative neoplasms in chronic phase and during disease progression (2011)

Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) are clonal myeloid disorders with increased production of terminally differentiated cells. The disease course is generally chronic, but some patients show disease progression (secondary myelofibrosis or accelerated phase) and/or leukemic transformation. We investigated chromosomal aberrations in 408 MPN samples using high-resolution single-nucleotide polymorphism microarrays to identify disease-associated somatic lesions. Of 408 samples, 37.5% had a wild-type karyotype and 62.5% harbored at least 1 chromosomal aberration. We identified 25 recurrent aberrations that were found in 3 or more samples. An increased number of chromosomal lesions was significantly associated with patient age, as well as with disease progression and leukemic transformation, but no association was observed with MPN subtypes, Janus kinase 2 (JAK2) mutational status, or disease duration. Aberrations of chromosomes 1q and 9p were positively associated with disease progression to secondary myelofibrosis or accelerated phase. Changes of chromosomes 1q, 7q, 5q, 6p, 7p, 19q, 22q, and 3q were positively associated with post-MPN acute myeloid leukemia. We mapped commonly affected regions to single target genes on chromosomes 3p (forkhead box P1 [FOXP1]), 4q (tet oncogene family member 2 [TET2]), 7p (IKAROS family zinc finger 1 [IKZF1]), 7q (cut-like homeobox 1 [CUX1]), 12p (ets variant 6 [ETV6]), and 21q (runt-related transcription factor 1 [RUNX1]). Our data provide insight into the genetic complexity of MPNs and implicate new genes involved in disease progression.
Substrate Specificity and Identification of Functional Groups of Homoserine Kinase from Escherichia coli (1996)

Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional group at the alpha-position has been replaced by an ester or by a hydroxymethyl group. Previous pH profile studies [Huo. X., & Viola, R. E. (1996) Arch. Biochem. Biophys. 330, 373-379] and chemical modification studies have suggested the involvement of histidinyl, lysyl, and argininyl residues in the catalytic activity of the enzyme. With the assistance of sequence alignments, several potential amino acids have been targeted for examination. Site-directed mutagenesis studies have confirmed a role for arginine-234 in the binding of the carboxyl group of L-homoserine, and the involvement of two histidine at the homoserine binding site. Mutations at these sites have led to the decoupling of the kinase activity from an inherent ATPase activity in the enzyme, and suggest the presence of independent domains for the binding of each substrate in homoserine kinase.
Auxiliary Image Regularization for Deep CNNs with Noisy Labels (2015)

Precisely-labeled data sets with sufficient amount of samples are very important for training deep convolutional neural networks (CNNs). However, many of the available real-world data sets contain erroneously labeled samples and those errors substantially hinder the learning of very accurate CNN models. In this work, we consider the problem of training a deep CNN model for image classification with mislabeled training samples - an issue that is common in real image data sets with tags supplied by amateur users. To solve this problem, we propose an auxiliary image regularization technique, optimized by the stochastic Alternating Direction Method of Multipliers (ADMM) algorithm, that automatically exploits the mutual context information among training images and encourages the model to select reliable images to robustify the learning process. Comprehensive experiments on benchmark data sets clearly demonstrate our proposed regularized CNN model is resistant to label noise in training data.
Accumulation ofS-Adenosyl-l-Methionine Enhances Production of Actinorhodin but Inhibits Sporulation inStreptomyces lividansTK23 (2003)

S-Adenosyl-L-methionine synthetase (SAM-s) catalyzes the biosynthesis of SAM from ATP and L-methionine. Despite extensive research with many organisms, its role in Streptomyces sp. remains unclear. In the present study, the putative SAM-s gene was isolated from a spectinomycin producer, Streptomyces spectabilis. The purified protein from the transformed Escherichia coli with the isolated gene synthesized SAM from L-methionine and ATP in vitro, strongly indicating that the isolated gene indeed encoded the SAM-s protein. The overexpression of the SAM-s gene in Streptomyces lividans TK23 inhibited sporulation and aerial mycelium formation but enhanced the production of actinorhodin in both agar plates and liquid media. Surprisingly, the overexpressed SAM was proven by Northern analysis to increase the production of actinorhodin through the induction of actII-ORF4, a transcription activator of actinorhodin biosynthetic gene clusters. In addition, we found that a certain level of intracellular SAM is critical for the induction of antibiotic biosynthetic genes, since the control strain harboring only the plasmid DNA did not show any induction of actII-ORF4 until it reached a certain level of SAM in the cell. From these results, we concluded that the SAM plays important roles as an intracellular factor in both cellular differentiation and antibiotic production in Streptomyces sp.
Synthetic Peptide Analogs of Intercellular Adhesion Molecule 1 (ICAM-1) Inhibit HIV-1 Replication in MT-2 Cells (1993)

On the basis of reports demonstrating possible roles for leukocyte function-associated antigen 1 (LFA-1) and intercellular adhesion molecule 1 (ICAM-1), the ligand for LFA-1, in human immunodeficiency virus type 1 (HIV-1) infection, we have explored the involvement of the ICAM-1 molecule by using selected synthetic peptides derived from the protein sequence. Replication was assessed in MT-2 cells, highly susceptible to HIV infection, in the presence of four synthetic peptides derived from the ICAM-1 amino acid sequence. This cell type was chosen for the ability to form marked syncytia on infection with cell-free virus. Under the conditions used, minimal or no cytotoxicity was observed with the peptides up to concentrations of 50 micrograms/ml. A peptide corresponding to a unique region of ICAM-1, JF9 [ICAM-1(367-394, A-378)], had little effect on virus replication despite its ability to inhibit cell-cell adhesion. In contrast, an N-terminal peptide, JF7B [ICAM-1(1-23)], consistently inhibited virus replication in MT-2 cells in a dose-dependent manner, as measured by cell-free reverse transcriptase (RT) activity (up to 70% inhibition), soluble virus antigen production (up to 60% inhibition), and syncytium formation (virtually complete inhibition up to 6 days post infection). Testing of W-CAM-1 antibody, and anti-ICAM-1 antibody that inhibits cell-cell adhesion, revealed no significant inhibitory effects on RT activity, virus antigen production, and syncytium formation in HIV-1-infected MT-2 cells at a level that markedly inhibited cell-cell adhesion (10 micrograms/ml).(ABSTRACT TRUNCATED AT 250 WORDS)
Structure and Dry Binding Activity of Different Polymers, Including Kollidon® VA 64 (2000)

The dry binding activity of copolyvidone (Kollidon VA 64), povidone (Kollidon 30), microcrystalline cellulose (Avicel PH-101), hydroxypropylmethylcellulose (HPMC) 2910 (Pharmacoat 606), and maltodextrin (Maldex 18) was investigated using a variety of formulations and methods. The effect of the dry binders in direct tableting and compaction was studied using a dicalcium phosphate formulation (water-insoluble ingredients) and a vitamin C formulation (water-soluble ingredients) applying three compression forces. The binder content was varied between 5% and 15% in both formulations, and the tablet properties were determined. All the tablets showed an improvement in mechanical properties (hardness, friability) with increasing dry binder concentration, with Kollidon VA 64 showing by far the greatest binding efficacy. A significant influence (prolongation) on drug release was observed only with HPMC 2910. The drying binding properties were analyzed for correlations with various powder and material properties. Especially, particle size, surface/surface structure, and plasticity were found to influence binding activity. The ideal dry binder should have small particles, high plasticity, and a large surface area.
Moderate cytotoxicity of proanthocyanidins to human tumour cell lines (1995)

Abstract In the present study the cytotoxicity of 16 proanthocyanidins was evaluated in GLC4, a human small cell lung carcinoma cell line, and in COLO 320, a human colorectal cancer cell line, using the microculture tetrazolium (MTT) assay. With IC 50 values ranging from 18 to &gt;200 μM following continuous incubation, the proanthocyanidins examined possess only moderate to low cytotoxicity. The highest cytotoxicity was found in a series of galloylated proanthocyanidins. An increase in the number of galloyl substituents increased cytotoxicity. Simple proanthocyanidins possess low cytotoxicity, that was independent of the stereochemistry. Compounds with doubly linked units had negligible cytotoxic effects, even at 200 μM, the highest concentration tested.
Update on the Management of Gonorrhea in Adults in the United States (2007)

Gonorrhea, the second most commonly reported notifiable disease, is an important cause of cervicitis, urethritis, and pelvic inflammatory disease. The selection of appropriate therapy for gonorrhea (i.e., safe, highly effective, single dose, and affordable) is complicated by the ability of Neisseria gonorrhoeae to develop resistance to antimicrobial therapies. This article reviews the key questions and data that informed the 2006 gonorrhea treatment recommendations of the Centers for Disease Control and Prevention. Key areas addressed include the criteria used to select effective treatment for gonorrhea, the level of antimicrobial resistance at which changing treatment regimens is recommended, the epidemiology of resistance, and the use of quinolones, cephalosporins, and other classes of antimicrobials for the treatment of uncomplicated gonorrhea.
Media Specialists Can Learn Web 2.0 Tools to Make Schools More Cool. (2007)

This author is teaching K-12 library media specialists about Web 2.0 tools, specifically blogs, wikis, and podcasts. Now the media specialists can use the applications to communicate with students on their own terms and to engage them more in their lessons.
Bone Anabolic Effects of S-40503, a Novel Nonsteroidal Selective Androgen Receptor Modulator (SARM), in Rat Models of Osteoporosis (2003)

A novel nonsteroidal androgen receptor (AR) binder, S-40503, was successfully generated in order to develop selective androgen receptor modulators (SARMs). We evaluated the binding specificity for nuclear receptors (NRs) and osteoanabolic activities of S-40503 in comparison with a natural nonaromatizable steroid, 5alpha-dihydrotestosterone (DHT). The compound preferentially bound to AR with nanomolar affinity among NRs. When S-40503 was administrated into orchiectomized (ORX) rats for 4 weeks, bone mineral density (BMD) of femur and muscle weight of levator ani were increased as markedly as DHT, but prostate weight was not elevated over the normal at any doses tested. In contrast, DHT administration caused about 1.5-fold increase in prostate weight. The reduced virilizing activity was clearly evident from the result that 4-week treatment of normal rats with S-40503 showed no enlargement of prostate. To confirm the bone anabolic effect, S-40503 was given to ovariectomized (OVX) rats for 2 months. The compound significantly increased the BMD and biomechanical strength of femoral cortical bone, whereas estrogen, anti-bone resorptive hormone, did not. The increase in periosteal mineral apposition rate (MAR) of the femur revealed direct bone formation activity of S-40503. It was unlikely that the osteoanabolic effect of the compound was attribute to the enhancement of muscle mass, because immobilized ORX rats treated with S-40503 showed a marked increase in BMD of tibial cortical bone without any actions on the surrounding muscle tissue. Collectively, our novel compound served as a prototype for SARMs, which had unique tissue selectivity with high potency for bone formation and lower impact upon sex accessory tissues.
Epidermal delivery of protein and DNA vaccines (2005)

Targeting vaccines to the skin epidermis results in the activation of an immune inductive site that is rich in antigen-presenting cells. The superficial location of the skin makes it accessible to vaccine delivery. However, it is difficult to access the epidermis using needle and syringe delivery, and vaccine antigens are too large to be effectively delivered using standard topical formulations. Needle-free vaccine delivery systems have been developed for efficient delivery of particulate vaccines into the epidermal tissue. Particle-mediated epidermal delivery of DNA vaccines is based on the delivery of DNA-coated gold particles directly into the cytoplasm and nuclei of living cells of the epidermis, facilitating DNA delivery and gene expression. Alternatively, protein vaccines can be formulated into a dense powder, which can be propelled into the skin epidermis by epidermal powder immunisation using similar delivery devices and principles, but in this instance the protein is delivered to the extracellular space. Preclinical and clinical data will be reviewed, demonstrating applications of epidermal vaccine delivery to a wide range of experimental infectious disease vaccines.
Battery Separators (2004)

The ideal battery separator would be infinitesimally thin, offer no resistance to ionic transport in electrolytes, provide infinite resistance to electronic conductivity for isolation of electrodes, be highly tortuous to prevent dendritic growths, and be inert to chemical reactions. Unfortunately, in the real world the ideal case does not exist. Real world separators are electronically insulating membranes whose ionic resistivity is brought to the desired range by manipulating the membranes thickness and porosity. It is clear that no single separator satisfies all the needs of battery designers, and compromises have to be made. It is ultimately the application that decides which separator is most suitable. We hope that this paper will be a useful tool and will help the battery manufacturers in selecting the most appropriate separators for their batteries and respective applications. The information provided is purely technical and does not include other very important parameters, such as cost of production, availability, and long-term stability. There has been a continued demand for thinner battery separators to increase battery power and capacity. This has been especially true for lithiumion batteries used in portable electronics. However, it is very important to ensure the continued safety of batteries, and this is where the role of the separator is greatest. Thus, it is essential to optimize all the components of battery to improve the performance while maintaining the safety of these cells. Separator manufacturers should work along with the battery manufacturers to create the next generation of batteries with increased reliability and performance, but always keeping safety in mind. This paper has attempted to present a comprehensive review of literature on separators used in various batteries. It is evident that a wide variety of separators are available and that they are critical components in batteries. In many cases, the separator is one of the major factors limiting the life and/or performance of batteries. Consequently, development of new improved separators would be very beneficial for the advanced high capacity batteries.
Reduction of nitroaromatic compounds by anaerobic bacteria isolated from the human gastrointestinal tract (1991)

Human intestinal microbial flora were screened for their abilities to reduce nitroaromatic compounds by growing them on brain heart infusion agar plates containing 1-nitropyrene. Bacteria metabolizing 1-nitropyrene, detected by the appearance of clear zones around the colonies, were identified as Clostridium leptum, Clostridium paraputrificum, Clostridium clostridiiforme, another Clostridium sp., and a Eubacterium sp. These bacteria produced aromatic amines from nitroaromatic compounds, as shown by thin-layer chromatography, high-pressure liquid chromatography, and biochemical tests. Incubation of three of these bacteria with 1-nitropyrene, 1,3-dinitropyrene, and 1,6-dinitropyrene inactivated the direct-acting mutagenicity associated with these compounds. Menadione and o-iodosobenzoic acid inhibited nitroreductase activity in all of the isolates, indicating the involvement of sulfhydryl groups in the active site of the enzyme. The optimum pH for nitroreductase activity was 8.0. Only the Clostridium sp. required added flavin adenine dinucleotide for nitroreductase activity. The nitroreductases were constitutive and extracellular. An activity stain for the detection of nitroreductase on anaerobic native polyacrylamide gels was developed. This activity stain revealed only one isozyme in each bacterium but showed that the nitroreductases from different bacteria had distinct electrophoretic mobilities.
The Next Generation of Sepsis Clinical Trial Designs (2014)

We need to improve our ability to define appropriate molecular targets for preclinical development and develop better methods to determine the clinical value of novel sepsis agents. Clinical trials must have realistic sample sizes and meaningful endpoints. Biomarker-driven studies should be considered to categorize specific "at risk" populations most likely to benefit from a new treatment. Innovations in clinical trial design such as parallel crossover design, alternative endpoints, or adaptive trials should be pursued to improve the outlook for future interventional trials in sepsis.
Phase I study of ONO-4007, a synthetic analogue of the lipid A moiety of bacterial lipopolysaccharide. (2000)

ONO-4007 is a synthetic analogue of the lipid A moiety of bacterial lipopolysaccharide, which exhibits antitumor activity by the induction of intratumoral tumor necrosis factor alpha, the potentiation of tumor-infiltrating macrophages, and the inhibition of angiogenesis. Interleukin (IL)-1 alpha, IL-6, and IL-12 induction by ONO-4007 activates cytotoxic natural killer cells to up-regulate IFN-gamma and nitric oxide synthase activity. ONO-4007 was given to 24 patients (13 males and 11 females; median age, 53 years) as a 30-min i.v. infusion on day 1, followed on day 15 by a first treatment cycle consisting of three weekly infusions at the same dose, followed by a rest period of 1 week. Cohorts of six patients received up to a maximum of four treatment cycles at increasing dose levels (75, 100, and 125 mg). The maximum tolerated dose was 125 mg, with grade 3 National Cancer Institute Common Toxicity Criteria toxicity (rigors with cyanosis) occurring in two of six patients at this dose level. An additional six patients were treated at 100 mg, the dose below the maximum tolerated dose. Other toxicities included grade 2 National Cancer Institute Common Toxicity Criteria myalgia, nausea, and hypotension. The pharmacokinetics of ONO-4007 appeared to be independent of dose and showed linearity with respect to time. ONO-4007 has a low systemic clearance (approximately 1.3 ml/min) and a small volume of distribution (5-8 liters) with a long t1/2 of 74-95 h. The administration of ONO-4007 was shown to result in a significant increase in circulating levels of tumor necrosis factor alpha and IL-6. No objective antitumor responses were observed. Seven patients maintained stable disease for at least two cycles, whereas five patients maintained stable disease for the full four-cycle duration of the study. Additional studies are required to determine the antitumor activity of ONO-4007.