gebhart/patrepeval huggingface dataset:
Structure of native Renilla reinformis luciferin (1977)

The structure of native luciferin from the bioluminescent coelenterate Renilla reniformis is shown to be 3,7-dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-one by mass spectral analysis of synthetic luciferin and the luciferin derived from a protein directly involved in the bioluminescent system. A previous report of the molecular weight of luciferin is shown to be incorrect by reexamination of the spectral data and by synthesis of two derivatives. Detailed analysis of kinetic, emission, and quantum yield data for the isolated and synthetic luciferins confirms this structure. Confirmation of this structure in a number of species from different phyla suggests a common substrate for a variety of bioluminescent marine organisms.
Effect of 1-Year Oral Administration of Dehydroepiandrosterone to 60- to 80-Year-Old Individuals on Muscle Function and Cross-sectional Area (2003)

The compensation of the deficit of dehydroepiandrosterone during aging using a 50-mg/d dose does not induce beneficial effects on muscle state in healthy subjects. The conditions in which dehydroepiandrosterone could contribute to preserve or improve muscle strength and morphological features still need to be determined.
Oxidative Stress in Neurodegenerative Diseases (1996)

Oxidative stress refers to the cytopathologic consequences of a mismatch between the production of free radicals and the ability of the cell to defend against them. Growing data from experimental models and human brain studies suggest oxidative stress may play an important role in neuronal degeneration in diseases such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis. Mitochondrial oxidative metabolism, nitric oxide, phospholipid metabolism, and proteolytic pathways are potential sources of intracellular free radicals. Alterations in free radical defense systems may also contribute to oxidative stress. A net increase in reactive oxygen species can produce damage to lipids, proteins, and DNA and induce necrosis or apoptosis. Elucidating the pathways important in the production of and defense from free radicals may be important in devising new pharmacologic strategies to slow or halt neuronal degeneration.
Molecular characterization of the pyrolysis of biomass (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular characterization of the pyrolysis of biomassRobert J. Evans and Thomas A. MilneCite this: Energy Fuels 1987, 1, 2, 123–137Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/ef00002a001https://doi.org/10.1021/ef00002a001research-articleACS PublicationsRequest reuse permissionsArticle Views4977Altmetric-Citations702LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Surface acoustic wave devices on bulk ZnO crystals at low temperature (2015)

Surface acoustic wave (SAW) devices based on thin films of ZnO are a well established technology. However, SAW devices on bulk ZnO crystals are not practical at room temperature due to the significant damping caused by finite electrical conductivity of the crystal. Here, by operating at low temperatures, we demonstrate effective SAW devices on the (0001) surface of bulk ZnO crystals, including a delay line operating at SAW wavelengths of λ = 4 and 6 μm and a one-port resonator at a wavelength of λ = 1.6 μm. We find that the SAW velocity is temperature dependent, reaching v ≈ 2.68 km/s at 10 mK. Our resonator reaches a maximum quality factor of Qi ≈ 1.5 × 105, demonstrating that bulk ZnO is highly viable for low temperature SAW applications. The performance of the devices is strongly correlated with the bulk conductivity, which quenches SAW transmission above 200 K.
Validation of a gene expression-based subclassification strategy for pediatric septic shock* (2011)

We have validated the existence of subclasses of children with septic shock based on a biologically relevant, 100-gene expression signature. The subclasses have relevant clinical differences.
Optimization strategies for the polymerase chain reaction. (1990)

The GeneAmp polymerase chain reaction (PCR) process has now become a key procedure in molecular biology research laboratories. The PCR technique is an in vitro method in which genomic or cloned target sequences are specifically enzymatically amplified as directed by a pair of oligonucleotide primers. This technique has been quite robust in the hands of the majority of researchers and is extremely flexible, as evidenced by the increasing number of related PCR formats (i.e., inverse PCR, anchored PCR, asymmetric PCR, labeled primer PCR and RNA-PCR). Today's applications include direct sequencing, genomic cloning, DNA typing, detection of infectious microorganisms, site-directed mutagenesis, prenatal genetic disease research, and analysis of allelic sequence variations. Scientists at Cetus and Perkin-Elmer have collaborated for several years to better understand the interacting biochemical and biophysical parameters which affect PCR optimization. Following are many of the current recommendations, offered with the caveat that our understanding of the PCR process is continually evolving.
Placental Enlargement in Women with Primary Maternal Cytomegalovirus Infection Is Associated with Fetal and Neonatal Disease (2006)

Primary maternal CMV infection and fetal or neonatal disease are associated with sonographically thickened placentas, which respond to administration of hyperimmune globulin. These observations suggest that many of the manifestations of fetal and neonatal disease are caused by placental insufficiency.
Facile and Large-Scale Production of ZnO/Zn−Al Layered Double Hydroxide Hierarchical Heterostructures (2006)

ZnO/Zn-Al layered double hydroxide (ZnO/Zn-Al LDH) hierarchical architecture, a new type of ZnO-based heterostructure, has been synthesized directly on an Al substrate via a facile solution phase process. The firecracker-like heterostructures consist of uniform ZnO nanorods orderly standing at the edges of two-dimensional (2D) surfaces of Zn-Al LDH nanoplatelets. Experimental result obtained from the early growth stage indicates that the underlying Zn-Al LDH nanoplatelet arrays are well constructed with their (00l) planes perpendicular to the surface of Al substrate. We propose that the "edge effect" of Zn-Al LDH and the "lattice match" between ZnO and Zn-Al LDH are vital to the growth of such heterostructures. The effects of total solution volume and NH3.H2O concentration on the formation of heterostructures are investigated. It is found that other LDH-based complex structures can also be achieved controllably by varying the mentioned experimental factors. Our work is the first demonstration of fabricating intricate ZnO/Zn-Al LDH heterostructures as well as well-defined Zn-Al LDH arrays on an Al substrate, for which several promising applications such as optoelectronics, biosensors, and catalysis can be envisioned.
A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells (2012)

Macrophages and dendritic cells (DCs) are key components of cellular immunity and are thought to originate and renew from hematopoietic stem cells (HSCs). However, some macrophages develop in the embryo before the appearance of definitive HSCs. We thus reinvestigated macrophage development. We found that the transcription factor Myb was required for development of HSCs and all CD11b(high) monocytes and macrophages, but was dispensable for yolk sac (YS) macrophages and for the development of YS-derived F4/80(bright) macrophages in several tissues, such as liver Kupffer cells, epidermal Langerhans cells, and microglia--cell populations that all can persist in adult mice independently of HSCs. These results define a lineage of tissue macrophages that derive from the YS and are genetically distinct from HSC progeny.
Aberrant expression of photoreceptor-specific calcium-binding protein (recoverin) in cancer cell lines. (2000)

Cancer-associated retinopathy (CAR) is an ocular manifestation of a paraneoplastic syndrome whereby immunological reactions to retinal antigens aberrantly expressed in tumor cells lead to the degeneration of retinal photoreceptor cells. In our previous study (H. Ohguro et al., Invest. Ophthalmol. Vis. Sci., 40: 82-89, 1999), recoverin, a retina-specific calcium-binding protein, and heat shock cognate protein 70 (hsc 70) were identified as autoantigens recognized by sera from patients with CAR. Therefore, we suggested that autoimmune reactions against both recoverin and hsc 70 might be involved in the pathogenesis of CAR. To elucidate the initial step of the molecular pathology of CAR, we examined the expression of recoverin and hsc 70 by reverse transcription-PCR and Western blot using cell lines of several kinds of cancers, including lung small cell carcinoma, lung adenocarcinoma, gastric cancer, pancreatic cancer, breast cancer, uterine cervical cancer, endometrial cancer, and leukemia. Recoverin was expressed in 21 of the 31 cancer cell lines. The expression levels of hsc 70 were significantly higher in cancer cell lines than in noncancerous cell lines. However, no difference in the expression levels of hsc 70 was observed between recoverin-positive and -negative cell lines. Immunofluorescence labeling by the affinity-purified recoverin antibody revealed the immunoreactivity to recoverin as a granular pattern within the cancer cells. Lung adenocarcinoma A549 cells, which did not express recoverin, exhibited a significant reduction in cell proliferation upon transfection with human recoverin cDNA. Taken together, our present data suggest that the retina-specific calcium-binding protein recoverin is expressed in more than 50% of a variety of cancer cells and may play a significant role in the cell proliferation of these tumor cells.
논문 : 효과적인 정보전달을 위한 온라인디자인 구성요소에 관한 연구 (2001)

21세기를 지식정보화(intelligent technology)시대라고 한다. 최근 국내외적으로 네트워크(network)의 환경이 질적, 양적으로 빠르게 발전하고 있다 현재 우리나라의 인터넷 사용자 수는 세계10위이고, 인프라 구축물은 세계4위, PC의 보유율은 세계5위인 것으로 나타나고 있다 이러한 현실로 인하여 국내 웹사이트는 다양한 컨텐츠(Contents)와 멀티미디어(multimedia)의 요소들로 채워지고 있으며 다양한 기능들의 추가로 인하여 철사미트의 인터페이스(Interface) 역시 많은 변화가 이루어지고 있다. 본 연구에서는 급속도로 변화되는 온라인 환경 속에서 효과적인 정보전달을 위한 디자인구성요소에 대한 연구를 위해 국내외 관련 서적 및 웹디자인 전문서적 등의 자료를 수집, 분석하고 실제 온라인 상에서의 웹사이트 활용사례를 분석, 활용하였다. 이에 효과적인 정보전달을 위한 온라인디자인을 위해서는 단순한 웹사이트디자인이 아닌 철저한 사용자 중심의 기획이 이루어져야하며 네비게이션의 효과적 활용 그리고 사용자와의 커뮤니케이션, 즉 사이트 내에서의 인터랙션이 적절한 조화를 이룰 때 온라인디자인의 구성요소로서의 웹사이트가 효과적인 정보전달 매체로서의 재역할을 다하게 될 것이다.
Comentarios a propósito de la STC de 20 de febrero de 2008 y su correlato en la STS de 12 de junio de 2008, sobre la prescripción del delito (2008)

El autor en el presente articulo realiza una exposicion del contenido de la doctrina jurisprudencial acerca de la naturaleza del acto interruptivo del plazo para la prescripcion del delito tras las sentencias del Tribunal Constitucional de 20 de febrero de 2008 y la sentencia del TS Sala Segunda, de 12 de junio de 2008 (caso «Urbanor» o «de los Albertos»)
Bosentan added to sildenafil therapy in patients with pulmonary arterial hypertension (2015)

The safety and efficacy of adding bosentan to sildenafil in pulmonary arterial hypertension (PAH) patients was investigated.In this prospective, double-blind, event-driven trial, symptomatic PAH patients receiving stable sildenafil (≥20 mg three times daily) for ≥3 months were randomised (1:1) to placebo or bosentan (125 mg twice daily). The composite primary end-point was the time to the first morbidity/mortality event, defined as all-cause death, hospitalisation for PAH worsening or intravenous prostanoid initiation, atrial septostomy, lung transplant, or PAH worsening. Secondary/exploratory end-points included change in 6-min walk distance and World Health Organization functional class at 16 weeks, change in N-terminal pro-brain natriuretic peptide (NT-proBNP) over time, and all-cause death.Overall, 334 PAH patients were randomised to placebo (n=175) or bosentan (n=159). A primary end-point event occurred in 51.4% of patients randomised to placebo and 42.8% to bosentan (hazard ratio 0.83, 97.31% CI 0.58-1.19; p=0.2508). The mean between-treatment difference in 6-min walk distance at 16 weeks was +21.8 m (95% CI +5.9-37.8 m; p=0.0106). Except for NT-proBNP, no difference was observed for any other end-point. The safety profile of bosentan added to sildenafil was consistent with the known bosentan safety profile.In COMPASS-2, adding bosentan to stable sildenafil therapy was not superior to sildenafil monotherapy in delaying the time to the first morbidity/mortality event.
Modeling Uncertainties in Aggregated Thermostatically Controlled Loads Using a State Queueing Model (2005)

To study the impacts of price responsive demand on the electric power system requires better load models. This paper discusses the modeling of uncertainties in aggregated thermostatically controlled loads using a state queueing (SQ) model. The cycling times of thermostatically controlled appliances (TCAs) vary with the TCA types and sizes, as well as the ambient temperatures. The random consumption of consumers, which shortens or prolongs a specific TCA cycling period, introduces another degree of uncertainty. By modifying the state transition matrix, these random factors can be taken into account in a discrete SQ model. The impacts of considering load diversity in the SQ model while simulating TCA setpoint response are also studied.
Development of HIF‐1 inhibitors for cancer therapy (2009)

Intratumour hypoxia has long been considered a driving force of tumour progression and a negative prognostic factor in human cancers. The discovery of hypoxia inducible factors (HIFs), which mediate transcriptional responses to changes in oxygen levels, has renewed enthusiasm for the discovery and development of targeted therapies exploiting the hypoxic tumour microenvironment. In spite of an ever increasing number of putative small molecule inhibitors of HIF, only few progress through pre-clinical and early clinical development. In this review, we will focus primarily on: (1) HIF inhibitors that have been more recently described and (2) small molecules targeting HIF that are being tested in early clinical trials or that are already approved for use in patients. A rigorous 'validation' of HIF targeted therapies in relevant pre-clinical models and eventually in pharmacodynamic-based early clinical trials is essential for 'credentialing' HIF-1 as a legitimate target that can be pharmacologically modulated in cancer patients.
Identification of a novel serum protein secreted by lung carcinoma cells (1986)

The murine anti-human lung tumor monoclonal antibody L3 recognizes antigens found both in the medium of cultured carcinoma cells and in normal human serum. Sequential immunoprecipitation experiments indicate that the L3 antigen is also recognized by a previously described monoclonal antibody directed against a melanoma-associated antigen [Natali, P. G., Wilson, B. S., Imai, K., Bigotti, A., & Ferrone, S. (1982) Cancer Res. 42, 583-589]. This antibody precipitated a Mr 76000 glycoprotein from metabolically labeled extracts of the lung carcinoma cell line Calu-1 and a Mr 94 000 glycoprotein from labeled culture medium. Pulse-chase experiments suggested a precursor-product relationship between these molecules. Analysis of glycosidase sensitivities of the two forms indicated that maturation of carbohydrate side chains correlated with the apparent increase in molecular weights. L3 antigenic activity, measured in a competitive radiometric cell binding assay, was purified more than 90-fold from serum-free medium of Calu-1 cells and more than 3000-fold from normal human serum. The major immunoreactive components purified from culture medium and serum were identical with respect to apparent molecular weight, electrophoretic mobility, pI, glycosidase sensitivity, and V8 protease fingerprints. In addition, the sequence of the amino-terminal 16 N-terminal amino acid residues of the major immunoreactive species from both sources was identical. The properties of the L3 antigen did not correspond to those of any known protein, suggesting that this serum protein has not been previously characterized.
Assessment of polyanion (BF4− and PF6−) substitutions in hybrid halide perovskites (2014)

The incorporation of tetrafluoroborate and hexafluorophosphate in halide perovskites is found to result in substantial band gap widening.
Transplantation of Ciliary Neurotrophic Factor-Expressing Adult Oligodendrocyte Precursor Cells Promotes Remyelination and Functional Recovery after SpinalCord Injury (2010)

Demyelination contributes to the dysfunction after traumatic spinal cord injury (SCI). We explored whether the combination of neurotrophic factors and transplantation of adult rat spinal cord oligodendrocyte precursor cells (OPCs) could enhance remyelination and functional recovery after SCI. Ciliary neurotrophic factor (CNTF) was the most effective neurotrophic factor to promote oligodendrocyte (OL) differentiation and survival of OPCs in vitro. OPCs were infected with retroviruses expressing enhanced green fluorescent protein (EGFP) or CNTF and transplanted into the contused adult thoracic spinal cord 9 d after injury. Seven weeks after transplantation, the grafted OPCs survived and integrated into the injured spinal cord. The survival of grafted CNTF-OPCs increased fourfold compared with EGFP-OPCs. The grafted OPCs differentiated into adenomatus polyposis coli (APC(+)) OLs, and CNTF significantly increased the percentage of APC(+) OLs from grafted OPCs. Immunofluorescent and immunoelectron microscopic analyses showed that the grafted OPCs formed central myelin sheaths around the axons in the injured spinal cord. The number of OL-remyelinated axons in ventrolateral funiculus (VLF) or lateral funiculus (LF) at the injured epicenter was significantly increased in animals that received CNTF-OPC grafts compared with all other groups. Importantly, 75% of rats receiving CNTF-OPC grafts recovered transcranial magnetic motor-evoked potential and magnetic interenlargement reflex responses, indicating that conduction through the demyelinated axons in VLF or LF, respectively, was partially restored. More importantly, recovery of hindlimb locomotor function was significantly enhanced in animals receiving grafts of CNTF-OPCs. Thus, combined treatment with OPC grafts expressing CNTF can enhance remyelination and facilitate functional recovery after traumatic SCI.
Physiological response to long-term peripheral and central leptin infusion in lean and obese mice (1997)

Recent data have identified leptin as an afferent signal in a negative-feedback loop regulating the mass of the adipose tissue. High leptin levels are observed in obese humans and rodents, suggesting that, in some cases, obesity is the result of leptin insensitivity. This hypothesis was tested by comparing the response to peripherally and centrally administered leptin among lean and three obese strains of mice: diet-induced obese AKR/J, New Zealand Obese (NZO), and Ay. Subcutaneous leptin infusion to lean mice resulted in a dose-dependent loss of body weight at physiologic plasma levels. Chronic infusions of leptin intracerebroventricularly (i.c.v.) at doses of 3 ng/hr or greater resulted in complete depletion of visible adipose tissue, which was maintained throughout 30 days of continuous i.c.v. infusion. Direct measurement of energy balance indicated that leptin treatment did not increase total energy expenditure but prevented the decrease that follows reduced food intake. Diet-induced obese mice lost weight in response to peripheral leptin but were less sensitive than lean mice. NZO mice were unresponsive to peripheral leptin but were responsive to i.c.v. leptin. Ay mice did not respond to subcutaneous leptin and were 1/100 as sensitive to i.c.v. leptin. The decreased response to leptin in diet-induced obese, NZO, and Ay mice suggests that obesity in these strains is the result of leptin resistance. In NZO mice, leptin resistance may be the result of decreased transport of leptin into the cerebrospinal fluid, whereas in Ay mice, leptin resistance probably results from defects downstream of the leptin receptor in the hypothalamus.
Risk Factors for Clostridium difficile Carriage and C. difficile-Associated Diarrhea in a Cohort of Hospitalized Patients (1990)

Aprospective cohort study of 399 consecutive patients in a single ward over an 11-month period was conducted to identify risk factors for nosocomial C. difficile colonization and diarrhea. The incidence of asymptomatic carriage was 13.0/100 patient admissions and the incidence of C. difficile-associated diarrhea was 7.8/100 patient admissions. Increased age and more severe underlying illness were associated with increased risk of C. difficile carriage and diarrhea. Multivariate models adjusting for age and severity of underlying disease associated two risk factors with asymptomatic C di fcile carriage: stool softeners (relative risk [RR] = 2.04) and antacids (RR = 1.80) . Five risk factors were associated with C. difficite-associated diarrhea:cephalosporinuse (RR = 2.07), penicillin use (RR = 3.41), enemas(RR = 3.26), gastrointestinal stimulants (RR = 3.06), and stool softeners (RR = 1.74). C. difficile was acommon nosocomial infection on this ward, resulting in asymptomatic carriage more often than diarrhea and accounting for one-fifth of all cases of nosocomial diarrhea.
Cancer Mortality Survey in a Spa Area (Misasa, Japan) with a High Radon Background (1992)

The 1952-88 cancer mortality records for inhabitants of the Misasa spa area, Japan, which has a high radon background, and a neighboring control area without any radon spa were analyzed (average outdoor Rn concentration: 26 mBq.liter-1 in Misasa vs. 11 mBq.liter-1 in the control area). Standardized mortality ratios (SMRs) for cancers of all sites were significantly lower among the inhabitants of both Misasa (male 0.538; female 0.463) and the control area (male 0.850; female 0.770), than in the whole Japanese population. Poisson regression analysis showed that the relative risks among the inhabitants of Misasa were significantly lower than in the control area for deaths from cancers of all sites (0.67) and stomach cancer (0.59). The relative risk of lung cancer death was also lower (0.55 times) in Misasa than in the control area, although the difference was not statistically significant. These results suggest that the linear no-threshold hypothesis for radiation risk may not be valid for exposure to low doses of radon.