gebhart/patrepeval huggingface dataset:
Occurrence of tumor-associated ganglioside antigens with Hanganutziu-Deicher antigenic activity on human melanomas. (1987)

The specificity of antibody to NeuGc alpha 2-3Gal beta 1-4Glc-cer (GM3(NeuGc] was carefully reexamined by the method of enzyme-immunostaining on a thin layer plate. The affinity-purified antibody was found to react with NeuGc alpha 2-8NeuGc alpha 2-3Gal beta 1-4Glc-cer (GD3(NeuGc-NeuGc] and NeuGc alpha 2-8NeuAc alpha 2-3Gal beta 1-4Glc-cer (GD3(NeuGc-NeuAc], but not with NeuAc alpha 2-8NeuGc alpha 2-3Gal beta 1-4Glc-cer (GD3(NeuAc-NeuGc)) or NeuAc alpha 2-8NeuAc alpha 2-3Gal beta 1-4Glc-cer (GD3(NeuAc-NeuAc]. From this result together with the previous results, it (GD3(NeuAc-NeuAc], From this result together with the previous results, it could be concluded that the antibody recognizes the outer portion of molecular species of sialic acids in the gangliosides. By using this antibody, the expression of Hanganutziu-Deicher (HD) gangliosides could be demonstrated in human malignant melanoma. The molecular species were different among individuals examined. Among HD-antigenic gangliosides, GM3(NeuGc) was commonly found in melanoma tissues. One of the patients examined expressed GD3(NeuGc-NeuGc) and GD 3(NeuGc-NeuAc), which may be characteristic gangliosides in human melanomas, since these gangliosides could not be detected in human colon cancer or human fetal tissues.
Angiogenesis for the treatment of vascular diseases. (1999)

Critical ischemia of the limbs or myocardium is frequently accompanied by diffuse distal vascular disease making it unapproachable by conventional revascularization techniques. Pharmacological treatment is available for coronary artery disease but there has been no effective medical therapy for advanced ischemia of the limbs. In the search for alternative treatments for patients with diffuse distal disease, recent developments in vascular biology have directed attention towards use of vascular growth factors. Therapeutic angiogenesis has shown promising results in early clinical studies as shown by improved clinical status and in some cases angiographic studies. We employed an angiogenic strategy that utilizes enhanced vascular endothelial growth factor (VEGF) in a fibrin network, in two patients with critical limb ischemia. Objectively, we were able to demonstrate angiographically the growth of new blood vessels after administration of VEGF and fibrin composite. Fibrin glue provides for the slow release of and prolongs the availability of VEGF, thereby sustaining angiogenesis resulting in improved oxygenation of ischemic tissue. Further investigations are warranted to validate if angiogenesis may increase blood flow in patients with advanced vascular disease.
Enhanced Expression of an α2,6-Linked Sialic Acid on MDCK Cells Improves Isolation of Human Influenza Viruses and Evaluation of Their Sensitivity to a Neuraminidase Inhibitor (2005)

The extensive use of neuraminidase (NA) inhibitors to treat influenza virus infections mandates close monitoring for resistant variants. Cultured cells do not provide a reliable means of evaluating the susceptibility of human influenza virus isolates to NA inhibitors. That is, the growth of such viruses in cell lines (e.g., Madin-Darby canine kidney [MDCK] cells) is not inhibited by these drugs, even though their sialidase activity is drug-sensitive. Matrosovich et al. (J. Virol. 77:8418-8425, 2003) showed that an MDCK cell line overexpressing the human beta-galactoside alpha2,6-sialyltransferase I (ST6Gal I) gene has the potential to assess the sensitivity of human influenza virus isolates to NA inhibitors, based on studies with a limited number of viruses. Here, we asked whether clinical isolates of influenza virus are universally sensitive to an NA inhibitor (oseltamivir) in an MDCK cell line expressing the ST6Gal I gene. The sensitivity of viruses to oseltamivir correlated with the sensitivity of viral sialidase to the compound, demonstrating the potential utility of this modified cell line for detecting NA inhibitor-resistant viruses. Moreover, in ST6Gal I-overexpressing cells, the growth of human influenza viruses was up to 2 logs higher than in MDCK cells. We conclude that the human ST6Gal I-expressing MDCK cell line is useful not only for evaluating their sensitivity to NA inhibitors, but also for isolation of influenza viruses from clinical samples.
Bone tissue engineering using human adipose‐derived stem cells and honeycomb collagen scaffold (2007)

Human adipose-derived stem cells (ASCs) have the capacity to regenerate and the potential to differentiate into multiple lineages of mesenchymal cells. The aim of this study was to investigate the possibility of using honeycomb collagen scaffold to culture ASCs in bone tissue engineering. The osteogenic capacity of ASCs in vitro, was confirmed by histology and measuring the expression of cbfa-1. After that, ASCs were cultured for up to 14 days in the honeycomb scaffold to allow a high density, three-dimensional culture. Scanning electron microscopy data showed that the scaffold was filled with the grown ASCs, and calcification, stained black with von Kossa, was confirmed. Furthermore, The ASC-loaded honeycomb collagen scaffolds cultured for 14 days were subcutaneously transplanted into nude mice, and excised after 8 weeks. Bone formation in vivo was examined using HE stain, von Kossa stain, and osteocalcin immunostain. Those histological views showed significant positive stains in the samples of osteogenic medium in the three types of stain. These results suggest that this carrier is a suitable scaffold for ASCs and will be useful as a three-dimensional bone tissue engineering scaffold in vitro and in vivo.
Regulation of bone mass by Wnt signaling (2006)

Wnt proteins are a family of secreted proteins that regulate many aspects of cell growth, differentiation, function, and death. Considerable progress has been made in our understanding of the molecular links between Wnt signaling and bone development and remodeling since initial reports that mutations in the Wnt coreceptor low-density lipoprotein receptor-related protein 5 (LRP5) are causally linked to alterations in human bone mass. Of the pathways activated by Wnts, it is signaling through the canonical (i.e., Wnt/beta-catenin) pathway that increases bone mass through a number of mechanisms including renewal of stem cells, stimulation of preosteoblast replication, induction of osteoblastogenesis, and inhibition of osteoblast and osteocyte apoptosis. This pathway is an enticing target for developing drugs to battle skeletal diseases as Wnt/beta-catenin signaling is composed of a series of molecular interactions that offer potential places for pharmacological intervention. In considering opportunities for anabolic drug discovery in this area, one must consider multiple factors, including (a) the roles of Wnt signaling for development, remodeling, and pathology of bone; (b) how pharmacological interventions that target this pathway may specifically treat osteoporosis and other aspects of skeletal health; and (c) whether the targets within this pathway are amenable to drug intervention. In this Review we discuss the current understanding of this pathway in terms of bone biology and assess whether targeting this pathway might yield novel therapeutics to treat typical bone disorders.
Adventage of mesenchymal stem cells (MSC) expansion directly from purified bone marrow CD105+ and CD271+ cells. (2008)

Average number of day 9 CFU-F colonies was the highest for CD271 positive fraction. Real-Time PCR analysis revealed expression of RUNX2, PPARgamma and N-cadherin in isolated cells, particularly high in CD271+ cells. Expression of CD105, CD166, CD44, CD73 antigens was comparable for all expanded populations (over 90%). We observed various levels of hematopoietic contamination with the highest numbers of CD45+ cells in MNC-MSC fraction and the lowest in CD105+ and CD271+ fractions. Cells of all the fractions were CD34 antigen negative. Expanded CD105 and CD271 populations showed higher level of RUNX2, osteocalcin, PTHR, leptin, PPARgamma2 and aggrecan1 genes except for alpha1 collagen. After osteogenic differentiation CD105+ and CD271+ populations showed lower expression of RUNX, PPARgamma2 and also lower expression of osteocalcin and PTHR than MNC, with comparable alpha1-collagen expression. Chondrogenic and adipogenic gene expression was higher in MNC. More clonogenic CD105+ and particularly CD271+ cells, which seem to be the most homogenous fractions based on Real-Time PCR and immunostaining data, are better suited for MSC expansion.
Catalytic hydrodeoxygenation and dealkylation of a lignin model compound (1987)

A comparison is presented of the hydrotreatment of 4-propylguaiacol (4PG) exploring two catalysts: MoS/sub 2/ on el-alumina and the dual functionality MoS/sub 2//NiS on phosphated el-alumina at 250-450C and 500 psig of hydrogen. The catalyst with acidic support was found to promote substantial dealkylation of 4PG into a number of alkylated phenols such as methyl- (predominantly meta- and para-), ethyl-, and methylpropyl-phenols. The main reaction product on the less acidic catalyst was 4-propylphenol. At 350C, mild deoxygenation to phenols can be maximized, whereas higher temperatures favor the formation of hydrocarbons, aromatic and saturated. Higher space velocities also favor mild deoxygenation to phenols.
Simulation based flow distribution network optimization for vacuum assisted resin transfer moulding process (2004)

In the vacuum assisted resin transfer moulding (VARTM) process, using a flow distribution network such as flow channels and high permeability fabrics can accelerate the resin infiltration of the fibre reinforcement during the manufacture of composite parts. The flow distribution network significantly influences the fill time and fill pattern and is essential for the process design. The current practice has been to cover the top surface of the fibre preform with the distribution media with the hope that the resin will flood the top surface immediately and penetrate through the thickness. However, this approach has some drawbacks. One is when the resin finds its way to the vent before it has penetrated the preform entirely, which results in a defective part or resin wastage. Also, if the composite structure contains ribs or inserts, this approach invariably results in dry spots. Instead of this intuitive approach, we propose a science-based approach to design the layout of the distribution network. Our approach uses flow simulation of the resin into the network and the preform and a genetic algorithm to optimize the flow distribution network. An experimental case study of a co-cured rib structure is conducted to demonstrate the design procedure and validate the optimized flow distribution network design. Good agreement between the flow simulations and the experimental results was observed. It was found that the proposed design algorithm effectively optimized the flow distribution network of the part considered in our case study and hence should prove to be a useful tool to extend the VARTM process to manufacture of complex structures with effective use of the distribution network layup.
Heterolayered hybrid dendrimers with optimized sugar head groups for enhancing carbohydrate–protein interactions (2017)

Novel heterolayered (“onion peel”) hybrid glycodendrimers containing optimised sugar head groups with galactoside and mannoside units with affinities for two different lectins.
It's a Start (1999)

Getting off to a good start in a class is a real challenge, however. A source of the difficulty is identified by Peter Elbow (1986), who in his superb collection of essays on teaching, Embracing Con traries, observes that we have two prima ry and conflicting roles as professors? gatekeeper and coach. As gatekeepers, we must set standards high enough to certify that our students will be qualified to enter their intended professions and the broader community of scholars. But as coaches, we must help our students surpass the hurdles we set for them as gatekeepers. Being a professor means walking a tightrope between these two roles. The key to success is to maintain a good balance. As Elbow observes, it is hard enough to fulfill these contrary roles over the course of a semester. The challenge we examine in this paper is that of establish
Tea pigments inhibit the production of type 1 (TH1) and type 2 (TH2) helper T cell cytokines in CD4+ T cells (2002)

Tea pigments are oxidized products of polyphenols derived from tea leaves (Camellia sinensis). Theaflavins are constituents of tea pigments with antioxidant, antineoplastic and antiinflammatory properties similar to their parent compounds. The biological properties of polyphenols and theaflavins have been linked to their capacity to inhibit the activation of nuclear factor-kappaB (NF-kappaB), a transcription factor, which is critically involved in the molecular regulation of a number of proinflammatory cytokines. The current study examines the requirement for NF-kappaB in the immunosuppressive effects mediated by tea antioxidants. Specifically, we tested the hypothesis that cytokines produced by type 1 (T(H1)) CD4(+) T cells which require NF-kappaB for gene expression, such as interleukin-2 (IL-2) and interferon gamma (IFN gamma), are selectively inhibited by tea pigments. We found that tea pigments potently suppress IL-2 secretion, IL-2 gene expression and the activation of NF-kappaB in murine spleens enriched for CD4(+) T cells, as expected. Consistent with our hypothesis, tea pigments also inhibited the induction of IFNgamma mRNA. However, the expression of the T(H2) cytokines IL-4 and IL-5, which lack functional NF-kappaB sites within their promoters was unexpectedly suppressed by tea pigments, as well. The results indicate that NF-kappaB may be only one of multiple transcription factors inhibited by tea pigments.
Room-temperature interconnection of electroplated Au microbump by means of surface activated bonding method (2002)

Although various bonding methods have been developed for flip-chip assembly, most of them cannot be applied to smaller pitch interconnection for the next generation. In the present study, a new bonding method, the surface activated bonding (SAB) is introduced. The feasibility of the SAB for bump bonding was investigated by some experiments. The Au electroplated bumps were prepared for experiments. The three different types of material, Au, Cu, and Al were used as contact metals. The reliability of interconnections was tested in temperature storage. As a result, we could achieved the bonding of microbumps with high strength and good electrical connection. It was also found that in the case of bump bonding, SAB can be done under relatively high vacuum pressure condition.
An arteriovenous loop in a protected space generates a permanent, highly vascular, tissue‐engineered construct (2006)

A major obstacle to 3-dimensional tissue engineering is incorporation of a functional vascular supply to support the expanding new tissue. This is overcome in an in vivo intrinsic vascularization model where an arteriovenous loop (AVL) is placed in a noncollapsible space protected by a polycarbonate chamber. Vascular development and hypoxia were examined from 3 days to 112 days by vascular casting, morphometric, and morphological techniques to understand the model's vascular growth and remodeling parameters for tissue engineering purposes. At 3 days a fibrin exudate surrounded the AVL, providing a scaffold to migrating inflammatory, endothelial, and mesenchymal cells. Capillaries formed between 3 and 7 days. Hypoxia and cell proliferation were maximal at 7 days, followed by a peak in percent vascular volume at 10 days (23.20+/-3.14% compared with 3.59+/-2.68% at 3 days, P<0.001). Maximal apoptosis was observed at 112 days. The protected space and spontaneous microcirculatory development in this model suggest it would be applicable for in vivo tissue engineering. A temporal window in a period of intense angiogenesis at 7 to 10 days is optimal for exogenous cell seeding and survival in the chamber, potentially enabling specific tissue outcomes to be achieved.
Completely Explosive Autonomous High-Voltage Pulsed-Power System Based on Shockwave Ferromagnetic Primary Power Source and Spiral Vector Inversion Generator (2006)

Novel explosive and conventional pulsed-power technologies were combined, and a series of explosive-driven high-voltage power supplies was designed, built, and tested. The power supply contained an explosive-driven high-voltage primary power source based on the fundamental physical effect of shockwave demagnetization of Nd <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2 </sub> Fe <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">14</sub> B high-energy ferromagnet and a power-conditioning stage. The volume of the energy-carrying ferromagnetic elements in the shockwave ferromagnetic generators (FMGs) was 8.75 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> . The power-conditioning stage was based on the spiral vector inversion generator (VIG). The combined FMG-VIG system demonstrated successful operation and good performance. The output-voltage pulse amplitude of the combined FMG-VIG system exceeded 40 kV, with a rise time of 6.2 ns. The methodology was developed for digital simulation of the operation of completely explosive FMG-VIG system. Experimental results obtained are in a good agreement with the results of digital calculations performed
VSV: L2-miss-driven variable supply-voltage scaling for low power (2003)

Energy efficient processor design is becoming more and more important with technology scaling and with high performance requirements. Supply-voltage scaling is an efficient way to reduce energy by lowering the operating voltage and the clock frequency of processor simultaneously. We propose a variable supply-voltage scaling (VSV) technique based on the following key observation: upon an L2 miss, the pipeline performs some independent computations but almost always ends up stalling and waiting for data, despite out-of-order issue and other latency-hiding techniques. Therefore, during an L2 miss we scale down the supply voltage of certain sections of the processor in order to reduce power dissipation while it carries on the independent computations at a lower speed. However, operating at a lower speed may degrade performance, if there are sufficient independent computations to overlap with the L2 miss. Similarly, returning to high speed may degrade power savings, if there are multiple outstanding misses and insufficient independent computations to overlap with them. To avoid these problems, we introduce two state machines that track parallelism on-the-fly, and we scale the supply voltage depending on the level of parallelism. We also consider circuit-level complexity concerns which limit VSV to two supply voltages, stability and signal-propagation speed issues which limit how fast VSV may transition between the voltages, and energy overhead factors which disallow supply-voltage scaling of large RAM structures such as caches and register file. Our simulation shows that VSV achieves an average of 20.7% total processor power reduction with 2.0% performance degradation in an 8-way, out-of-order-issue processor that implements deterministic clock gating and software prefetching, for those SPEC2K benchmarks that have high L2 miss rates. Averaging across all the benchmarks, VSV reduces total processor power by 7.0% with 0.9% performance degradation.
Microwave integrated circuits using thin-film BST (2002)

We discuss the development and potential applications of a microwave varactor technology using thin-film BST on inexpensive substrates. BST thin-films have been developed and optimized specifically for microwave integrated circuits, using both MOCVD-grown and RF magnetron-sputtered films. The material optimization efforts have concentrated on achieving high tunability and simultaneous low loss, and also developing robust electrode systems for circuit fabrication on silicon substrates. We present advances in monolithic microwave integrated capacitors and application to high performance and low-cost phase-shifters circuits. Efforts in frequency multiplication devices will also be presented.
Palladium(II)-Catalyzed Asymmetric 1,3-Dipolar Cycloaddition of Nitrones to 3-Alkenoyl-1,3-oxazolidin-2-ones (1999)

Chiral phosphinepalladium(II)-catalyzed asymmetric 1,3-dipolar cycloaddition of nitrones to α,β-unsaturated carboxylic acid derivatives has been investigated. In the presence of a catalytic amount of [Pd(NCMe)<sub>2</sub>{(<i>S</i>)-tolbinap}](BF<sub>4</sub>)<sub>2</sub> [TolBINAP = 2,2'-bis(di-<i>p</i>-tolylphosphino)-1,1'-binaphthyl], the reaction of 3-alkenoyl-1,3-oxazolidin-2-ones as dipolarophiles and N-substituted <i>N</i>-benzylidenenitrones has been successfully performed to give isoxazolidine derivatives in high yields with high enantioselectivities. For example, 3-((2,5-dimethyl-3-phenylisoxazolidin-4-yl)carbonyl)-1,3-oxazolidin-2-one was obtained from the reaction of <i>N</i>-benzylidenemethylamine <i>N</i>-oxide and 3-crotonoyl-1,3-oxazolidin-2-one in 89% yield with 60% endo selectivity and 91% ee of the endo isomer. The cycloaddition of <i>N</i>-benzylidenebenzylamine <i>N</i>-oxide and 3-crotonoyl-1,3-oxazolidin-2-one afforded 3-((2-benzyl-5-methyl-3-phenylisoxazolidin-4-yl)carbonyl)-1,3-oxazolidin-2-one in 94% yield with 93% endo selectivity and 89% ee of the endo isomer. Remarkably, the endo/exo selectivity of the products depended on the N-substituent group of the nitrones. These selectivities were explained using molecular modeling.
Hyperosmolar blood-brain barrier disruption in baboons: an in vivo study using positron emission tomography and rubidium-82 (1996)

Hyperosmolar blood-brain barrier (BBB) disruption remains controversial as an adjuvant therapy to increase delivery of water-soluble compounds to extracellular space in the brain in patients with malignant brain tumors. To understand the physiological effects of BBB disruption more clearly, the authors used positron emission tomography (PET) to study the time course of BBB permeability in response to the potassium analog rubidium-82 (82Rb, halflife 75 seconds) following BBB disruption in anesthetized adult baboons. Mannitol (25%) was injected into the carotid artery and PET scans were performed before and serially at 8-to 15-minute intervals after BBB disruption. The mean influx constant (K1), a measure of permeability-surface area product, in ipsilateral, mannitol-perfused mixed gray- and white-matter brain regions was 4.9 +/-2.4 microliter/min/ml (+/- standard deviation) at baseline and increased more than 100% (delta K1=9.4 +/-5.1 microliter/min/ml, 18 baboons) in brain perfused by mannitol. The effect of BBB disruption on K1 correlated directly with the total amount of mannitol administered (p< 0.005). Vascular permeability returned to baseline with a halftime of 24.0 +/- 14.3 minutes. The mean brain plasma volume rose by 0.57 +/- 0.34 ml/100 ml in ipsilateral perfused brain following BBB disruption. This work provides a basis for the in vivo study of permeability changes induced by BBB disruption in human brain and brain tumors.