gebhart/patrepeval huggingface dataset:
Characterization/Modeling of Wire Screen Insulation for Deep-Water Pipes (2006)

The amount of oil consumption has been rapidly increas ing around the world; consequently , industries have expanded to offshore exploration in to deep sea waters . At sea floor depths , pipe insulation is essential to prevent the blockage with in the pipe due to paraffin build -up within crude oil . To maintain a proper inner crude oil temperature , ab ove the paraffin formation point, insulation techniques have been used to overcome the thermal issue , but these technologies still have limitations. In this study, the objective was to develop an analytical model for a wire screen joint which contained bo th nonconforming and conforming interfaces , and then compare the predicted results to experimental measured data for validation. The experimental study for this novel insulation techn ology consisted of a coaxial pipe fabricated from P110 -4140 steel with a low conductivity wire screen (Stainless Steel) as the interstitial insulation material inserted at the annulus .
Identification of FcγRIIa as the ITAM-bearing receptor mediating αIIbβ3 outside-in integrin signaling in human platelets (2008)

Immunoreceptor tyrosine-based activation motif (ITAM)-containing proteins have recently been demonstrated in macrophages and neutrophils to be required for cell surface integrins to transmit activation signals into the cell. To identify ITAM-bearing proteins that mediate signaling via the platelet-specific integrin alphaIIbbeta3, fibrinogen binding was induced by (1) allowing platelets to spread directly on immobilized fibrinogen, or (2) activating the PAR1 thrombin receptor on platelets in suspension. Both initiated strong, ligand binding-dependent tyrosine phosphorylation of the ITAM-bearing platelet Fc receptor, FcgammaRIIa, as well as downstream phosphorylation of the protein tyrosine kinase Syk and activation of phospholipase Cgamma2 (PLCgamma2). Addition of Fab fragments of an FcgammaRIIa-specific monoclonal antibody strongly inhibited platelet spreading on immobilized fibrinogen, as well as downstream tyrosine phosphorylation of FcgammaRIIa, Syk, and PLCgamma2, and platelets from a patient whose platelets express reduced levels of FcgammaRIIa exhibited markedly reduced spreading on immobilized fibrinogen. Finally, fibrinogen binding-induced FcgammaRIIa phosphorylation did not occur in human platelets expressing a truncated beta3 cytoplasmic domain. Taken together, these data suggest that ligand binding to platelet alphaIIbbeta3 induces integrin cytoplasmic domain-dependent phosphorylation of FcgammaRIIa, which then enlists selected components of the immunoreceptor signaling cascade to transmit amplification signals into the cell.
Multistep Navigation and the Combinatorial Control of Leukocyte Chemotaxis (1997)

Cells migrating within tissues may encounter multiple chemoattractant signals in complex spatial and temporal patterns. To understand leukocyte navigation in such settings, we have explored the migratory behavior of neutrophils in model scenarios where they are presented with two chemoattractant sources in various configurations. We show that, over a wide range of conditions, neutrophils can migrate "down" a local chemoattractant gradient in response to a distant gradient of a different chemoattractant. Furthermore, cells can chemotax effectively to a secondary distant agonist after migrating up a primary gradient into a saturating, nonorienting concentration of an initial attractant. Together, these observations suggest the potential for cells' step-by-step navigation from one gradient to another in complex chemoattractant fields. The importance of such sequential navigation is confirmed here in a model system in which neutrophil homing to a defined domain (a) requires serial responses to agonists presented in a defined spatial array, and (b) is a function of both the agonist combination and the sequence in which gradients are encountered. We propose a multistep model of chemoattractant-directed migration, which requires that leukocytes display multiple chemoattractant receptors for successful homing and provides for combinatorial determination of microenvironmental localization.
Stiffness estimation of a tripod-based parallel kinematic machine (2002)

Presents a simple yet comprehensive approach that enables the stiffness of a tripod-based parallel kinematic machine to be quickly estimated. The approach arises from the basic idea for the determination of the equivalent stiffness of a group of serially connected linear springs and can be implemented in two steps. In the first step, the machine structure is decomposed into two substructures associated with the machine frame and parallel mechanism. The stiffness models of these two substructures are formulated by means of the virtual work principle. This is followed by the second step that enables the stiffness model of the machine structure as a whole to be achieved via linear superposition. The three-dimensional representations of the machine stiffness within the usable workspace are depicted and the contributions of different component rigidities to the machine stiffness are discussed. The results are compared with those obtained through experiments.
Ig light chains are secreted predominantly as monomers (1996)

Ig light (L) chains are secreted not only as part of assembled Ab molecules, but also as free L chains, the latter process being involved in the pathology of several diseases. The secretion competence of free L chains distinguishes them from free subunits of other oligomeric proteins, which are usually retained intracellularly. We used several techniques to test the idea that secretion of free L chains is dependent on dimerization. Coexpression of pairs of L chains, differing in only one amino acid, which alters the secretory phenotype, shows that these L chains behave independently: the wild-type chains are secreted, whereas the mutants are retained intracellularly. A survey of kappa- or lambda-producing cell lines by nonreducing gel electrophoresis shows that a negligible fraction of these L chains exists as disulfide-bonded dimers. Moreover, chemical cross-linking and density gradient centrifugation demonstrate that there is no significant pool of noncovalent L chain dimers. Noncovalent heterodimers can be detected readily between a kappa-chain and a chimera consisting of a heavy chain variable domain linked to the kappa-chain constant domain. This confirms that noncovalent L chain homodimers would have been detected if they were present. These findings about the association state of free L chains are independent of the host cell, as they are observed in both myeloma cells and COS fibroblasts. We conclude that L chain dimerization is a rare event that neither facilitates secretion nor is required for it.
Recent advances in understanding muscular dystrophy. (1992)

A report on the development of a schizophrenia-like disorder after the start of etanercept in a middle-aged woman who had no history of psychiatric illness. Etanercept was discontinued and she was treated with sulpiride for 6 months. There has been no relapse of psychosis since etanercept was discontinued, despite being off sulpiride for 3 months. Unfortunately, subsequent treatment she has had for her rheumatoid arthritis has not afforded the same level of symptom control as etanercept. This report points to the need for more research into the differential impact of reduced-tumor necrosis factor (TNF) α levels in the different regions of the brain. It highlights the need for pharmacovigilance especially in individuals on etanercept and other TNF inhibitors. It also highlights the need to systematically consider differential diagnosis before arriving at a diagnosis of psychiatric illness even in the presence of highly suggestive constellation of symptoms.
Save Pave the World (2012)

Carbon dioxide may be the ultimate industrial waste product. Could tropical corals provide a trick for locking it away in our highways?
Phage Display of Recombinant Antibodies Toward Burkholderia pseudomallei Exotoxin (2002)

We have used the phagemid pComb3H to construct recombinant phages displaying the single chain variable fragment (ScFv) towards exotoxin of Burkholderia pseudomallei. Variable heavy and light chain fragments were amplified from the hybridoma 6E6A8F3B line, with a wide spectrum of primers specific to mouse antibody genes. Through overlapping extension polymerase chain reaction, the heavy and light chain fragments were linked to form the ScFv which was subsequently cloned into the phage display vector and transformed into ER2537 cells to yield a complexity of 10(8) clones. The transformants were screened by four rounds of biopanning against the exotoxin and resulted in selective enrichment of exotoxin-binding antibodies by 301 fold. The phage pool from the final round of selection displayed antibodies of high-affinity to the exotoxin as demonstrated by ELISA. Several clones were selected randomly from this pool and analysed by restriction enzyme digestion, fingerprinting and sequencing. Restriction analysis confirmed that all clones carried a 700-800 bp insert whose sequences, in general, corresponded to that of mouse IgG. Fingerprinting profiles delineated the antibodies into two families with different CDR sequences.
Serum Levels of the Interleukin-1 Receptor Family Member ST2, Cardiac Structure and Function, and Long-Term Mortality in Patients With Acute Dyspnea (2009)

Among dyspneic patients with and without acute heart failure, sST2 concentrations are associated with prevalent cardiac abnormalities on echocardiography, a more decompensated hemodynamic profile and are associated with long-term mortality, independent of echocardiographic, clinical, or other biochemical markers of risk.
Tradeoffs in compressing virtual machine checkpoints (2013)

Checkpoint replication is a prevalent way of maintaining virtual machine availability in the presence of host failures. Since checkpoint replication can impose heavy load on network resources, checkpoint compression has been suggested to reduce network usage. This paper presents the first detailed evaluation and characterization of the effectiveness and overheads of checkpoint compression methods for various workloads frequently seen in high-availability systems. We propose a lightweight compression method that exploits similarities in checkpoints to eliminate redundant network traffic, and compare it with two well-known methods, gzip and delta compression. Our results show that gzip and delta compression reduce network traffic significantly for various workloads, but incur high CPU and memory overheads, respectively. The proposed similarity compression is most effective for VM clusters running homogeneous workloads, while using both CPU and memory efficiently. Based on our extensive evaluation, we suggest guidelines for selecting and using these compression methods.
Safety Assessment of Amino Acid Alkyl Amides as Used in Cosmetics (2017)

The Cosmetic Ingredient Review Expert Panel (Panel) reviewed the product use, formulation, and safety data of 115 amino acid alkyl amides, which function as skin and hair conditioning agents and as surfactants-cleansing agents in personal care products. Safety test data on dermal irritation and sensitization for the ingredients with the highest use concentrations, lauroyl lysine and sodium lauroyl glutamate, were reviewed and determined to adequately support the safe use of the ingredients in this report. The Panel concluded that amino acid alkyl amides are safe in the present practices of use and concentration in cosmetics, when formulated to be nonirritating.
Metal ion-enhanced helicity in synthetic peptides containing unnatural, metal-ligating residues (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetal ion-enhanced helicity in synthetic peptides containing unnatural, metal-ligating residuesFuqiang Ruan, Yanqiu Chen, and Paul B. HopkinsCite this: J. Am. Chem. Soc. 1990, 112, 25, 9403–9404Publication Date (Print):December 1, 1990Publication History Published online1 May 2002Published inissue 1 December 1990https://doi.org/10.1021/ja00181a058RIGHTS & PERMISSIONSArticle Views601Altmetric-Citations179LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (280 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Dynamic adaptive streaming over HTTP -- (2011)

In this paper, we provide some insight and background into the Dynamic Adaptive Streaming over HTTP (DASH) specifications as available from 3GPP and in draft version also from MPEG. Specifically, the 3GPP version provides a normative description of a Media Presentation, the formats of a Segment, and the delivery protocol. In addition, it adds an informative description on how a DASH Client may use the provided information to establish a streaming service for the user. The solution supports different service types (e.g., On-Demand, Live, Time-Shift Viewing), different features (e.g., adaptive bitrate switching, multiple language support, ad insertion, trick modes, DRM) and different deployment options. Design principles and examples are provided.
Detection of embedded ultra-subwavelength-thin dielectric features using elongated photonic nanojets (2010)

Photonic nanojets have been previously shown (both theoretically and experimentally) to be highly sensitive to the presence of an ultra-subwavelength nanoscale particle within the nanojet. In the present work, photonic nanojets elongated by almost an order of magnitude (relative to the latest previously published work) are found to possess another key characteristic: they are sensitive to the presence of ultra-subwavelength nanoscale thin features embedded within a dielectric object. This additional characteristic of photonic nanojets is demonstrated through comparisons between fundamentally different 3-D and corresponding 1-D full Maxwell's equations finite-difference time-domain (FDTD) models.
Substrate Profiling of Cysteine Proteases Using a Combinatorial Peptide Library Identifies Functionally Unique Specificities (2006)

The substrate specificities of papain-like cysteine proteases (clan CA, family C1) papain, bromelain, and human cathepsins L, V, K, S, F, B, and five proteases of parasitic origin were studied using a completely diversified positional scanning synthetic combinatorial library. A bifunctional coumarin fluorophore was used that facilitated synthesis of the library and individual peptide substrates. The library has a total of 160,000 tetrapeptide substrate sequences completely randomizing each of the P1, P2, P3, and P4 positions with 20 amino acids. A microtiter plate assay format permitted a rapid determination of the specificity profile of each enzyme. Individual peptide substrates were then synthesized and tested for a quantitative determination of the specificity of the human cathepsins. Despite the conserved three-dimensional structure and similar substrate specificity of the enzymes studied, distinct amino acid preferences that differentiate each enzyme were identified. The specificities of cathepsins K and S partially match the cleavage site sequences in their physiological substrates. Capitalizing on its unique preference for proline and glycine at the P2 and P3 positions, respectively, selective substrates and a substrate-based inhibitor were developed for cathepsin K. A cluster analysis of the proteases based on the complete specificity profile provided a functional characterization distinct from standard sequence analysis. This approach provides useful information for developing selective chemical probes to study protease-related pathologies and physiologies.
Modulation of the enzymatic activity of papain by interdomain residues remote from the active site (1994)

The two main catalytic residues Cys25 and His159 of the monomeric cysteine protease papain are located on different walls of a cleft formed by two domains. This topology suggests a possible relationship between relative domain organization and catalytic mechanism. The effect on enzymatic parameters of structural modifications at various locations of the two-domain interface of papain was examined by individual or double replacements by Ala of pairs of interacting residues. Most modifications had no effect on enzyme activity. However, the enzyme's substrate turnover (kcat) decreased following simultaneous alteration of the two most conserved residues, forming an apolar contact located 15 A away from the active site. The pH activity profile of the double mutant was unchanged, indicating a conserved ionization state of the active site thiolate-imidazolium ion pair. This state is strongly dependent on the distance separating the two residues, thus suggesting that the active site geometry has not been significantly altered. Efficient enzymatic activity in papain requires more than a correct active site geometry and is influenced by domain packing properties in a region remote from the active site.
Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome (2004)

Mx proteins belong to the dynamin superfamily of high molecular weight GTPases and interfere with multiplication of a wide variety of viruses. Earlier studies show that nuclear mouse Mx1 and human MxA designed to be localized in the nucleus inhibit the transcription step of the influenza virus genome. Here we set a transient influenza virus transcription system using luciferase as a reporter gene and cells expressing the three RNA polymerase subunits, PB1, PB2 and PA, and NP. We used this reporter assay system and nuclear-localized MxA proteins to get clues for elucidating the anti-influenza virus activity of MxA. Nuclear-localized VP16-MxA and MxA-TAg NLS strongly interfered with the influenza virus transcription. Over-expression of PB2 led to a slight resumption of the transcription inhibition by nuclear MxA, whereas over-expression of PB1 and PA did not affect the MxA activity. Of interest is that the inhibitory activity of the nuclear MxA was markedly neutralized by over-expression of NP. An NP devoid of its C-terminal region, but containing the N-terminal RNA binding domain, also neutralized the VP16-MxA activity in a dose-dependent manner, whereas an NP lacking the N-terminal region did not affect the VP16-MxA activity. Further, not only VP16-MxA but also the wild-type MxA was found to interact with NP in influenza virus-infected cells. This indicates that the nuclear MxA suppresses the influenza virus transcription by interacting with not only PB2 but also NP.
Structural Properties and Antibacterial Effects of Hydrophobic and Oleophobic Sol−Gel Coatings for Cotton Fabrics (2009)

In a continuation of previous studies, the wetting properties of the hydrophobic diureapropyltriethoxysilane [bis(aminopropyl)-terminated polydimethylsiloxane (1000)] (PDMSU) sol-gel hybrid, which forms washing-resistant water-repellent finishes on cotton fabrics, were further investigated. The addition of 1H,1H,2H,2H-perfluorooctyltriethoxysilane (PFOTES) to PDMSU resulted in a highly apolar low-energy surface on aluminum with gammaStotal equal to 14.5 mJ/m2 and a DetlaGiwi value of -82 mJ/m2. Mixed PFOTES-PDMSU finishes applied on cotton fabrics increased the water contact angles (thetaw) from approximately 130 degrees (PDMSU) to 147 degrees, also imparting oleophobicity (thetadiiodomethane=130 degrees, thetan-hexadecane=120 degrees) to the finished cotton fabrics. Washing caused breakage of the coating's integrity as established from SEM, which was attributed to the partial removal of PFOTES from the composite films, also shown by subtractive IR attenuated total reflectance (ATR) and XPS spectral measurements made on washed and unwashed fabrics. The antibacterial properties of the PFOTES-PDMSU-finished fabrics were assessed with the transfer method (EN ISO 20743:2007), revealing that the reduction of Escherichia coli bacteria on unwashed cotton fabrics was nearly 100%. Moreover, for washed (10 times) cotton fabrics a much higher bacterial reduction was noted for the PFOTES-PDMSU finishes (60.6+/-10.8%), surpassing PDMSU (30.4+/-6.1%) and commercial fluoroalkoxysilane (FAS) (21.9+/-5.7%) finishes. The structure of PFOTES-PDMSU gels, xerogels, and the corresponding coatings was investigated by analyzing the 29Si NMR and IR ATR spectra and comparing them with the spectra of PFOTES and octameric (T8) PFOTES based polyhedra. The results revealed the tendency of PFOTES to condense in octameric silsesquioxane polyhedra (T8), coexisting in the PDMSU sol-gel network with cyclic tetramers (T4(OH)4) and open cube-like species (T7(OH)3). The presence of -OH-functionalized PFOTES silsesquioxanes, established even in coatings heat-treated at 140 degrees C (15 min), also explained the excellent washing fastness of PFOTES finishes on cotton fabrics. The regenerative nature of the water- and oil-repellent properties of washed PFOTES-PDMSU-finished cotton fabrics was attributed to the surface mobility of the T8 PFOTES based polyhedra, ousted from the coating interior during consecutive washings.
Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells (2009)

These modified fibroblasts are useful as a genetically-defined feeder cell line for reproducible and cost-effective culture of both hESC and iPS cells.
Frequencies of the Blood Groups ABO, Rhesus, D Category VI, Kell, and of Clinically Relevant High-Frequency Antigens in South-Western Germany (1995)

We presented refined estimates of ABO, Rhesus D and Kell blood group frequencies and established reliable frequency estimates for Rhesus haplotype and some rare blood groups. The prevalence of D category VI was about 0.02%, which necessitates specific detection for Rh-D-negative transfusion therapy. A protocol is presented for Rh D typing in transfusion recipients. which obviates the need for an antiglobulin test.
Role of gap junctions in chronic pain (2011)

Gap junctions are specialized transmembrane channels that allow rapid electrical signalling and direct intercellular communication for maintenance and coordination of normal cellular activities and homeostasis. Although gap junction channels in the nervous system mediate intercellular coupling between glial cells and between neurons, they also contribute to the spread of secondary damage and inflammation under pathological conditions. There is now evidence of the involvement of gap junctions in chronic pain caused by nervous system damage or tissue inflammation. In this Mini-Review, we highlight recent studies demonstrating the dynamic plasticity of gap junctions in response to nervous system injury and the effects of gap junction blockade on neuronal survival and modulation of pain in animal models of neuropathic and inflammatory pain. The involvement of dorsal root ganglia and spinal cord gap junctions in mediating chronic pain and the potential for targeting connexins as a novel modality for the treatment of intractable pain syndromes arising from nervous system injury and disorders are discussed.