gebhart/patrepeval huggingface dataset:
The dielectrophoresis enrichment of CD34+ cells from peripheral blood stem cell harvests. (1996)

There is considerable interest in isolating the CD34+ cell population from leukaemic patients undergoing peripheral blood stem cell harvests. The techniques currently available make use of antibodies specific to the CD34+ surface markers. However, all of these techniques involve disturbance of the cell surface, are time-consuming and relatively expensive. In this study, we have used dielectrophoresis, which does not rely on the presence of cell-specific markers, to separate CD34+ cells from peripheral blood stem cell harvest samples containing an untreated natural mixed cell population. The separation is achieved by exploiting differences in the inherent dielectric properties of the various cell types. Samples obtained from peripheral blood stem cell harvests were resuspended in medium with a conductivity of less than 50 microS/cm and introduced into the dielectrophoretic separation chamber. Alternating field frequencies, from 500 kHz to 5 kHz, were used to collect cell fractions which were analysed by FACS, using a CD34-specific antibody, to quantify the CD34+ population within the fractions. On average a nearly five-fold increase in the frequency of the CD34+ cell population was observed in the fractions collected within the 50-10 kHz range. For this dielectrophoretic separation technique to be suitable in harvesting CD34+ cells for transplantation, it is important to demonstrate that the cells remain viable after the separation process. Cells obtained from each fraction grew when plated in colony assay cultures, GM-CFU and BFU-E, demonstrating that the cells remain normal, viable and capable of colony formation when cultured for 2 weeks. The number of colonies formed correlated with the percentage of CD34+ cells in each fraction. The dielectrophoretic separation technique is simple to operate, the separation is fast, the procedure non-invasive and although not tested has the potential to be incorporated as a batch-wise online facility with the standard harvesting equipment to increase the yield and speed of CD34+ cells in the PBSC harvest.
The Facts on File dictionary of chemistry (2000)

In this reference more than 3000 entries reflect modern chemical nomenclature and up-to-date information on commonly used chemical terms and the properties of elements.
Effect of case selection on the performance of computer‐aided detection schemes (1994)

The choice of clinical cases used to train and test a computer-aided diagnosis (CAD) scheme can affect the test results (i.e., error rate). In this study, we deliberately modified the components of our testing database to study the effects of this modification on measured performance. Using a computerized scheme for the automated detection of breast masses from mammograms, it was found that the sensitivity of the scheme ranged between 26% and 100% (at a false positive rate of 1.0 per image) depending on the cases used to test the scheme. Even a 20% change in the cases comprising the database can reduce the measured sensitivity by 15%-25%. Because of the strong dependence of measured performance on the testing database, it is difficult to estimate reliably the accuracy of a CAD scheme. Furthermore, it is questionable to compare different CAD schemes when different cases are used for testing. Sharing databases, creating a common database, or using a quantitative measure to characterize databases are possible solutions to this problem. However, none of these solutions exists or is practiced at present. Therefore, as a short-term solution, it is recommended that the method used for selecting cases, and histograms or mean and standard deviations of relevant image features be reported whenever performance data are presented.
d -Dimer Testing to Determine the Duration of Anticoagulation Therapy (2006)

Patients with an abnormal D-dimer level 1 month after the discontinuation of anticoagulation have a significant incidence of recurrent venous thromboembolism, which is reduced by the resumption of anticoagulation. The optimal course of anticoagulation in patients with a normal D-dimer level has not been clearly established. (ClinicalTrials.gov number, NCT00264277 [ClinicalTrials.gov].).
Molecular characterization of regenerated cardiomyocytes derived from adult mesenchymal stem cells (2002)

We recently isolated a cardiomyogenic (CMG) cell line from murine bone marrow stroma, and in this paper characterize regenerated cardiomyocytes derived from adult mesenchymal stem cells at the molecular level. Stromal cells were immortalized, exposed to 5-azacytidine, and repeatedly screened for spontaneously beating cells. CMG cells began to beat spontaneously after 2 weeks, and beat synchronously after 3 weeks. They exhibited sinus-node-like or ventricular-cell-like action potentials. Analysis of the isoforms of contractile protein genes, such as of myosin and alpha-actin, indicated that their phenotype was similar to that of fetal ventricular cardiomyocytes. The cells expressed Nkx2.5, GATA4, TEF-1, and MEF2-C mRNA before 5-azacytidine exposure, and MEF2-A and MEF2-D after exposure. CMG cells expressed alpha1A, alpha1B, and alpha1D-adrenergic receptor mRNA prior to differentiation, and beta1, beta2-adrenergic and M1, M2-muscarinic receptors after acquiring the cardiomyocyte phenotype. Phenylephrine induced phosphorylation of ERK1/2, and the phosphorylation was inhibited by prazosin. Isoproterenol increased the cAMP level 38-fold and beating rate, cell motion, %shortening, and contractile velocity by 48%, 38%, 27%, and 51%, respectively, and the increases were blocked by CGP20712A (beta1-selective blocker). Carbachol increased IP3 32-fold, and the increase was inhibited by AFDX116 (M2-selective blocker). These findings demonstrated that the regenerated cardiomyocytes were capable of responding to adrenergic and muscarinic stimulation. This new cell line provides a model for the study of cardiomyocyte transplantation.
The role of T cells in the immunopathogenesis of rheumatoid arthritis. New perspectives (1997)

No Abstract.
Cache replacement based on reuse-distance prediction (2007)

Several cache management techniques have been proposed that indirectly try to base their decisions on cacheline reuse-distance, like Cache Decay which is a postdiction of reuse-distances: if a cacheline has not been accessed for some ldquodecay intervalrdquo we know that its reuse-distance is at least as large as this decay interval. In this work, we propose to directly predict reuse-distances via instruction-based (PC) prediction and use this information for cache level optimizations. In this paper, we choose as our target for optimization the replacement policy of the L2 cache, because the gap between the LRU and the theoretical optimal replacement algorithm is comparatively large for L2 caches. This indicates that, in many situations, there is ample room for improvement. We evaluate our reusedistance based replacement policy using a subset of the most memory intensive SPEC2000 and our results show significant benefits across the board.
Two promoters that map to 5'-sequences of the human p53 gene are differentially regulated during terminal differentiation of human myeloid leukemic cells. (1989)

p53 is overexpressed in many transformed cells and expression of the gene is known to alter during terminal differentiation of cells in culture. Through analysis of recombinant vectors expressing the chloramphenicol acetyl transferase (CAT) gene we found that two promoters map to the 5'-portion of the human p53 oncogene. One promoter, p53p1, maps upstream of the non-coding first exon and the second, p53p2, maps within the first intron. By primer extension analysis of cellular RNA from a number of human cell lines, we found that p53p2 is a functional promoter in vivo. In order to test whether differential regulation of these promoters may be correlated with the control of expression of the p53 gene during differentiation, we have measured the activity of the two promoters by their ability to direct expression of the CAT gene during terminal differentiation of the human promyelocytic leukemia cell line HL-60. HL-60 cells stably harboring Epstein-Barr virus-derived recombinant plasmids that express the CAT gene from either p53p1 or p53p2 were induced to undergo terminal differentiation by a variety of chemical inducers to either granulocytes or monocytes and expression of the CAT gene was measured. The results indicate that while expression of p53p1 remained constant, expression from p53p2 was induced 5- to 10-fold during differentiation of these cells to either granulocytes or monocytes. Similarly, the endogenous p53p2 was found to be induced in HL-60 cells undergoing differentiation. Although the product of the p53p2 initiated transcript has not yet been characterized these results indicate that altered regulation of these two promoters may be important in modulating the expression of mRNA from this gene during terminal differentiation.
Organization of the Human LU Gene and Molecular Basis of the Lua/Lub Blood Group Polymorphism (1997)

The Lutheran (Lu) blood group antigens and the B-cell adhesion molecule (B-CAM) epithelial cancer antigen are carried by recently cloned integral glycoproteins that belong to the Ig superfamily. We have previously shown that the Lu and B-CAM antigens are encoded by the same gene, LU, and that alternative splicing of the primary transcript most likely accounts for the presence of both antigens on two isoforms that differ by the length of their cytoplasmic tails. In the present report, we isolated the human LU gene by cloning a 20-kb HindIII fragment from Lu(a - b+) genomic DNA. The LU gene is organized into 15 exons distributed over 12.5 kb. Alternative splicing of intron 13 generates the 2.5- and 4.0-kb transcript spliceoforms encoding the long tail and the short tail Lu polypeptides, respectively. Sequencing of the major mRNA species (2.5 kb) amplified from human bone marrow, kidney, placenta, and skeletal muscle did not suggest the presence of tissue-specific Lu glycoprotein isoforms. The same transcription initiation point, located 22 bp upstream from the initiation codon, was characterized in several tissues. In agreement with the wide tissue distribution of the Lu messengers, the GC-rich proximal 5' flanking region of the LU gene does not contain TATA or CAAT boxes, but includes several potential binding sites for the ubiquitous Sp1 transcription factor. In addition, the distal 5' region, encompassing nucleotides -673 to -764, contains clustered binding sequences for the GATA, CACCC, and Ets transcription factors. Analysis of the coding sequences amplified from genomic DNA of Lu(a + b-) or Lu(a - b+) donors showed a single nucleotide change in exon 3 (A229G) that correlates with an Aci I restriction site polymorphism and results in a His77Arg amino-acid substitution. Polymerase chain reaction/restriction fragment length polymorphism analysis indicated that the A229G mutation is associated with the Lu(a)/Lu(b) blood group polymorphism. When expressed in Chinese hamster ovary (CHO) cells, Lu cDNAs carrying the A229 or the G229 produced cell surface proteins that reacted with anti-Lu(a) or anti-Lu(b) antibodies, respectively, showing that these nucleotides specify the Lu(a) and Lu(b) alleles of the Lutheran blood group locus. CHO cells expressing recombinant short-tail or long-tail Lu glycoproteins reacted as well with anti-Lu as with anti-B-CAM antibodies, providing the definitive proof that the Lu blood group and B-CAM antigens are carried by the same molecules.
Commodity Overview: Prospects for World Economic Recovery and Commodity Prices (2002)

Following a sharp weakening of world economic growth, a crucial question facing commodity producers and exporters is the future direction of commodity price movements. Lower commodity prices, if they persist, will lead to significantly lower export revenues and returns.
Structured Knowledge Distillation for Semantic Segmentation (2019)

In this paper, we investigate the issue of knowledge distillation for training compact semantic segmentation networks by making use of cumbersome networks. We start from the straightforward scheme, pixel-wise distillation, which applies the distillation scheme originally introduced for image classification and performs knowledge distillation for each pixel separately. We further propose to distill the structured knowledge from cumbersome networks into compact networks, which is motivated by the fact that semantic segmentation is a structured prediction problem. We study two such structured distillation schemes: (i) pair-wise distillation that distills the pairwise similarities, and (ii) holistic distillation that uses adversarial training to distill holistic knowledge. The effectiveness of our knowledge distillation approaches is demonstrated by extensive experiments on three scene parsing datasets: Cityscapes, Camvid and ADE20K.
Structural and Functional Analysis of an mRNP Complex That Mediates the High Stability of Human β-Globin mRNA (2001)

Human globins are encoded by mRNAs exhibiting high stabilities in transcriptionally silenced erythrocyte progenitors. Unlike alpha-globin mRNA, whose stability is enhanced by assembly of a specific messenger RNP (mRNP) alpha complex on its 3' untranslated region (UTR), neither the structure(s) nor the mechanism(s) that effects the high-level stability of human beta-globin mRNA has been identified. The present work describes an mRNP complex assembling on the 3' UTR of the beta-globin mRNA that exhibits many of the properties of the stability-enhancing alpha complex. The beta-globin mRNP complex is shown to contain one or more factors homologous to alphaCP, a 39-kDa RNA-binding protein that is integral to alpha-complex assembly. Sequence analysis implicates a specific 14-nucleotide pyrimidine-rich track within its 3' UTR as the site of beta-globin mRNP assembly. The importance of this track to mRNA stability is subsequently verified in vivo using mice expressing human beta-globin transgenes that contain informative mutations in this region. In combination, the in vitro and in vivo analyses indicate that the high stabilities of the alpha- and beta-globin mRNAs are maintained through related mRNP complexes that may share a common regulatory pathway.
Electron Hopping through Films of Arenethiolate Monolayer-Protected Gold Clusters (2002)

Electron hopping in films of arenethiolate (benzylthiolate, phenylethylthiolate, phenylbutanethiolate, and cresolthiolate) monolayer-protected cluster molecules (MPCs) is investigated through measurements of solid-state electronic conductivity. Electron donor−acceptor hopping rates between the Au cores of arenethiolate MPCs exceed those of previously studied solid-state alkanethiolate MPC films, but the electronic coupling term, β = 0.8 Å-1, is nearly the same. Rate constants range from 108 to 1011 s-1 across the series of arenethiolate MPCs; for the case of cresolthiolate, the rate corresponds to a single molecule resistance of ∼107 Ω/cresolthiolate ligand. The 4−8 kJ/mol activation-barrier energies for electron hopping are generally in line with the Marcus theory prediction. The low barrier energies and large rate constants arise from a combination of the low dielectric medium environment of the reactants (the MPC cores) and the partly aromatic tunneling bridges. The sharp increase in film conductivity upon substituting arenethiolate ligands for >50% of the hexanethiolates on a hexanethiolate-protected MPC suggests a percolation effect.
Recurrent diabetes mellitus in the pancreas iso- and allograft. A light and electron microscopic and immunohistochemical analysis of four cases. (1985)

Four patients with type 1 diabetes mellitus received segmental pancreatic grafts. The donors were HLA-identical twins in three patients and an HLA-identical sibling in one. Each patient had normal glucose metabolism in the posttransplantation period but impaired graft function developed after 6 to 12 weeks. Complete loss of function developed in three patients. The fourth patient received immunosuppressive therapy but continues to require a low dose of insulin 15 months following transplantation. Pancreatic graft biopsy at the time of declining graft function in three patients revealed a mononuclear cell infiltrate centered upon islets consisting of variable numbers of T11 (pan T), OKT8 (suppressor-killer), OKT9 (transferrin receptor), OKT10 (activated), and HLA-DR-reactive mononuclear cells, as well as 63D3 and OKM1 reactive monocytes. Biopsies obtained following loss of graft function revealed resolution of the inflammatory process and selective destruction of all islet beta-cells in two patients, whereas graft biopsy in one patient demonstrated a mononuclear cell infiltrate in islets containing demonstrable beta-cells but no infiltrate in islets without beta-cells. Following immunosuppressive therapy the fourth patient showed resolution of the insulitis and destruction of beta-cells in 70% of the islets. The variable numbers of beta-cells observed in the remaining islets likely account for the relatively low amount of exogenous insulin required by this patient. There was no immunohistologic evidence of humoral mediated immune reaction in any of the biopsies. It is postulated that selective beta-cell destruction was a consequence of cell-mediated immunity leading to recurrent diabetes mellitus.
Inhibition of Bax Channel-Forming Activity by Bcl-2 (1997)

Proteins of the Bcl-2 family are intracellular membrane-associated proteins that regulate programmed cell death (apoptosis) either positively or negatively by as yet unknown mechanisms. Bax, a pro-apoptotic member of the Bcl-2 family, was shown to form channels in lipid membranes. Bax triggered the release of liposome-encapsulated carboxyfluorescein at both neutral and acidic pH. At physiological pH, release could be blocked by Bcl-2. Bcl-2, in contrast, triggered carboxyfluorescein release at acidic pH only. In planar lipid bilayers, Bax formed pH- and voltage-dependent ion-conducting channels. Thus, the pro-apoptotic effects of Bax may be elicited through an intrinsic pore-forming activity that can be antagonized by Bcl-2.
7-ketocholesterol induces cell apoptosis by activation of nuclear factor kappa B in mouse macrophages. (2010)

We investigated the molecular mechanisms responsible for the induction of apoptosis in mouse monocytic macrophage cell line J774A.1 stimulated by 7-ketocholesterol (7-KC). Cell apoptosis was detected by Annexin V-propidium iodide (PI) staining. The DNA-binding activity of nuclear factor kappa B (NF-kappaB) was assessed by electrophoretic mobility shift assay (EMSA). Results showed that 7-KC-stimulation in J774A.1 cells activated NF-kappaB, which is involved in cell apoptosis, in a time- and dose-dependent manners. 7-KC was also found to increase the binding activity of NF-kappaB to specific DNA binding sites, a possible mechanism for the induction of the cell apoptosis. Moreover, these effects were partially inhibited by pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor. Taken together, 7-KC may be an important factor in atherosclerosis due to the ability of 7-KC to induce cell apoptosis, which is at least partially mediated through the activation of NF-kappaB.
Laron Syndrome (Primary Growth Hormone Resistance or Insensitivity): The Personal Experience 1958–2003 (2004)

Clinical and laboratory investigations starting in 1958 of a group of dwarfed children resembling isolated GH deficiency but who had very high serum levels of GH led to the description of the syndrome of primary GH resistance or insensitivity (Laron syndrome) and subsequently to the discovery of its molecular defects residing in the GH receptor and leading to an inability of IGF-I generation. With the biosynthesis of IGF-I in 1986, therapeutic trials started. Continuously more and more patients are being diagnosed in many parts of the world with a variety of molecular defects. This syndrome proved to be a unique model that enables the study of the consequences of GH receptor defects, the physiopathology of GH-IGF-I disruption, and comparison of the GH-independent IGF-I effects. This review presents the personal experience gained from the study follow-up and treatment of the 60 patients followed up for many years in the Israeli cohort.
Clearance and tissue distribution of staphylococcal enterotoxin A in the rat and potential use of adsorbents for removal from plasma (1993)

Summary Many of the profound effects of staphylococcal septicaemia are thought to be the result of entry of enterotoxins into the systemic circulation. The aim of this study was to investigate the disposition of staphylococcal enterotoxin A (SEA) in the rat and its possible removal from blood. SEA labelled with 125I was administered intravenously (250 µg/kg) to rats. The blood clearance of SEA showed a biphasic pattern; an initial fast disapperance (half-life c. c min) was followed by a slower one (half-life c. 2 h). Thirty minutes after injection of 125I-labelled SEA, most of the radioactivity was concentrated in the kidneys, indicating that renal excretion was the main route of elimination of SEA. The adsorption capacities of polymer-coated activated charcoal (DHP-1 and Adsorba 150C), uncharged resin (Amberlite XAD-7), anion exchange resin (Dowex-1) and polymyxin B matrix were assessed by measurement of the equilibrium adsorption isotherms for SEA. DHP-1 charcoal, Amberlite XAD-7 resin and Dowex-1 resin adsorbed similar amounts of SEA in human plasma. Plasma perfusion experiments were performed in vitro with small columns containing either charcoal or resin adsorbents. Over 4 h perfusion, DHP-1 charcoal removed 50% of the initial amount of 125I-SEA, Adsorba 150C charcoal 8·1 of SEA and Amberlite XAD-7 resin 32·5% of SEA. These results suggest that it may be feasible to develop the adsorbent columns for direct removal of SEA from the plasma of patients with staphylococcal septicaemia.
Shaping of subreflectors in cassegrainian antennas for maximum aperture efficiency (1973)

This paper describes a systematic technique for obtaining high aperture efficiency in a Cassegrainian feed system by special shaping of the subreflector. Theoretical expressions are derived that determine the subreflector shape, which gives a uniform illumination of a paraboloid for a given primary feed pattern. Experimental data are then presented, which demonstrate the enhanced performance of a Cassegrainian feed system using a subreflector shaped according to the theoretical expressions.
Lossless and low-crosstalk characteristics in an InP-based 2*2 optical switch (1993)

A lossless and low-crosstalk optical switch is most desirable for large-scale photonic networks. To realize such a switch, a traveling-wave amplifier is integrated into the slip waveguide of a carrier-injection type optical single-slip structure (S/sup 3/) switch. We demonstrate fiber-to-fiber lossless and low-crosstalk (40 dB ON/OFF ratio) characteristics of two of four elements in a 2*2 crossbar optical switch that integrates four amplifiers and eight Y-branch optical switches.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>