gebhart/patrepeval huggingface dataset:
Interaction of GYKI 52466, a selective non-competitive antagonist of AMPA/kainate receptors, with conventional antiepileptic drugs in amygdala-kindled seizures in rats. (2002)

GYKI 52466 [1,4-aminophenyl)-4-methyl-7,8-methylenedioxy-5H-2,3-benzodiazepine], a non-competitive AMPA/kainate receptor antagonist, administered i.p. at the dose of 5 mg/kg, exerted a significant anticonvulsant effect, as it decreased seizure and afterdischarge durations, being ineffective at 2 mg/kg. Subsequently, GYKI 52466 (2 mg/kg) was combined with antiepileptic drugs at doses ineffective in fully kindled rats. Co-administration of GYKI 52466 with clonazepam (0.003 mg/kg i.p.) resulted in a significant reduction of seizure severity (by 20%), seizure duration (by 31%) and afterdischarge duration (by 24%). Co-injection of GYKI 52466 with valproate (75 mg/kg i.p.) also resulted in the respective 8%, 16%, and 17% reductions of the three studied seizure parameters. No protection was observed when GYKI 52466 was co-administered with carbamazepine (20 mg/kg i.p.), phenobarbital (20 mg/kg i.p.), or diphenylhydantoin (40 mg/kg i.p.). Combinations of GYKI 524662 with antiepileptic drugs did not cause any significant motor (rotarod test) or long-term memory deficits (passive avoidance task). Only GYKI 52466 administered alone at 5 mg/kg, caused a significant impairment of retention in amygdala-kindled rats. The interaction at a pharmacokinetic level, at least in case of the combination of GYKI 52466 with valproate, can be excluded because GYKI 52466 did not interfere with the free plasma level of valproate. These results give further support to the idea of a potential clinical benefits of the combined treatment of AMPA/kainate receptor antagonists with some antiepileptic drugs.
Self‐Assembled Monolayers That Transduce Enzymatic Activities to Electrical Signals (2003)

A dynamic substrate that transduces a biological activity into an electrical signal has been developed (see scheme). The enzymatic conversion of a redox-inactive substrate presented at a self-assembled monolayer produced a redox-active product that is monitored electrochemically in real time. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2003/z50862_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Thymoglobulin-induced severe cardiovascular reaction and acute renal failure in a patient scheduled for orthotopic liver transplantation. (2006)

Antithymocyte globulin (ATG) has been shown to be effective as a pretreatment immunosuppressive agent in liver transplantation because of the ability to wean tacrolimus monotherapy after 4 months in pretreated recipients. However, the use of ATG can be complicated by serious side effects. Reported side effects include severe cardiopulmonary reactions, adult respiratory distress syndrome and hematological disorders. We report a case of a patient with a medical history of cirrhosis scheduled for orthotopic liver transplantation that, during the operation, showed swelling, hyperthermia, tachycardia and hypotension after the administration of ATG. Acute renal failure (ARF) was another serious side effect that our patient developed during ICU stay; we ascribed the occurrence of ARF to the serum sickness disease triggered by the ATG administration. Only one case has been reported of ARF after ATG-therapy before our experience. Therefore, severe hyperthermia and signs of cardiovascular dysfunction early after the beginning of ATG administration should be carefully evaluated and may need to consider the immediate ATG therapy withdrawal to prevent ARF.
lnterleukin-8 in Airway Inflammation in Patients with Asthma and Chronic Obstructive Pulmonary Disease (1996)

We have investigated whether IL-8 is present in airway secretions from patients with asthma and chronic obstructive pulmonary disease (COPD) to obtain information on its possible role in airway inflammation in obstructive airways disease. In the bronchoalveolar lavage fluid (BALF) from 11 clinically stable patients with asthma the levels of IL-8 were increased compared to 10 healthy subjects (median: controls 21.5 pg/ml, asthma 244 pg/ml: p < 0.005). In the patients with asthma the levels of IL-8 correlated with the percentage neutrophils in the BALF (r = 0.81; p < 0.001) and with a parameter of the permeability of the respiratory membrane, the quotient (alpha 2-macroglobulin in BALF)/(alpha 2-macroglobulin in serum) (r = 0.66; p < 0.025). In the sputum sol phase of 9 patients with symptomatic asthma the levels of IL-8 were lower than in 9 patients with COPD (asthma: 6.4 ng/ml; COPD: 16.3 ng/ml; p < 0.02) and significantly correlated with those of neutrophilic myeloperoxidase (MPO; r = 0.85; p < 0.005). The increased levels of IL-8 in the airway secretions from both patients with asthma and COPD may be markers of an ongoing inflammatory process, which is more pronounced in patients with COPD. In patients with asthma the strong correlation between the levels of IL-8 and the percentage neutrophils and/or the levels of MPO points to a role of IL-8 in the recruitment and activation of neutrophils in the airway lumen.
Collaborative restricted boltzmann machine for social event recommendation (2016)

The development of social networks has not only improved the online experience, but also stimulated the advances in knowledge mining so as to assist people in planning their offline social events. Users can explore their favorite events, such as celebrations and symposiums, through the pictures and the posts from their friends on social networks. An effective event recommendation can offer great convenience for both event organizers and participants, which yet remains extremely challenging due to a wide range of practical concerns. In this paper we propose a novel recommendation framework, which combines the information from multiple sources and establishes a connection between the online knowledge and the event participation.
Experimental Evidence of Temperature Gradient Effects on the Neutral Gas Penetration into a Magnetized Rotating Plasma (1983)

The experimental evidence of temperature gradient effects on the neutral gas penetration into a plasma is presented. It is found that an increased temperature gradient reduces the neutral gas content in the partially ionized boundary layer of a cold gas blanket system. A strong modification of the rotating plasma equilibrium is then observed. Such an equilibrium is a sensitive function of the neutral gas concentration both in the plasma and its boundary layers. The moderate values of the centrifugal force which act both on the ions and neutrals do not appreciably influence the plasma permeability to neutral gas. It is further observed that an acceleration of neutrals takes place in a rotating plasma.
Mammalian Scratch: A neural-specific Snail family transcriptional repressor (2001)

Members of the Snail family of zinc finger transcription factors are known to play critical roles in neurogenesis in invertebrates, but none of these factors has been linked to vertebrate neuronal differentiation. We report the isolation of a gene encoding a mammalian Snail family member that is restricted to the nervous system. Human and murine Scratch (Scrt) share 81% and 69% identity to Drosophila Scrt and the Caenorhabditis elegans neuronal antiapoptotic protein, CES-1, respectively, across the five zinc finger domain. Expression of mammalian Scrt is predominantly confined to the brain and spinal cord, appearing in newly differentiating, postmitotic neurons and persisting into postnatal life. Additional expression is seen in the retina and, significantly, in neuroendocrine (NE) cells of the lung. In a parallel fashion, we detect hScrt expression in lung cancers with NE features, especially small cell lung cancer. hScrt shares the capacity of other Snail family members to bind to E-box enhancer motifs, which are targets of basic helix--loop--helix (bHLH) transcription factors. We show that hScrt directly antagonizes the function of heterodimers of the proneural bHLH protein achaete-scute homolog-1 and E12, leading to active transcriptional repression at E-box motifs. Thus, Scrt has the potential to function in newly differentiating, postmitotic neurons and in cancers with NE features by modulating the action of bHLH transcription factors critical for neuronal differentiation.
Fluorescent dyes for lymphocyte migration and proliferation studies (1999)

Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the most versatile in terms of long-term tracking of lymphocytes and their ability to quantify lymphocyte proliferation. They are the intracellular covalent coupling dye carboxyfluorescein diacetate succinimidyl ester (CFSE) and the membrane inserting dye PKH26. Both dyes have the advantage that they can be used to track cell division, both in vitro and in vivo, due to the progressive halving of the fluorescence intensity of the dyes in cells after each division. However, CFSE appears to have the edge over PKH26 based on homogeneity of lymphocyte staining and cost. Two other fluorescent dyes, although not suitable for lymphocyte proliferation studies, are valuable tracking dyes for short-term (up to 3 day) lymphocyte migration experiments, namely the DNA-binding dye Hoechst 33342 and the cytoplasmic dye calcein. In the future it is highly likely that additional fluorescent dyes, with different spectral properties to CFSE, will become available, as well as membrane inserting fluorescent dyes that more homogeneously label lymphocytes than PKH26.
Glycerolysis of fats and methyl esters — Status, review and critique (1982)

Abstract With the possible exception of catalytic hydrogenation, perhaps no unit operation within the realm of oleochemistry is as thoroughly complex as that of glycerolysis. Among the misconceptions and half‐truths that prevail concerning the glycerolysis of fats are the notions that it involves a strictly random distribution of acyl groups among all of the available hydroxyl groups, that the solubility of glycerol in the fat at the reaction temperature determines the yield of monoglyceride that may be obtained, that the advantageous effects of the Law of Mass Action can be realized only when the reaction media is in ultimate homogeneity, in other words, complete mutual solubility, and, more importantly, that there is an equivalence of emulsification properties for the chief products of glycerolysis, i.e., the α‐and β‐monoglycerides, in both food and industrial emulsification. Numerous examples from international literature establish the limitations which prevail in temperature, agitation and use of excess of glycerol in batch glycerolysis reactions, but the practical limits for glycerolysis under superemulsification conditions remain to be established. The disadvantages of glycerolysis in homogeneous solvents still are insufficient to justify the use of those that are available, but the use of both pressure and gaseous catalysts such as carbon dioxide appear to offer the greatest hope for improvement in yields. Substantial energy savings may dictate the choice of methyl ester glycerolysis processing for future plants, especially those in the international sphere. Pros and cons of monoglyceride analytical methodology are evaluated.
Granulocyte Function in Women With Diabetes and Asymptomatic Bacteriuria (1997)

The prevalence of asymptomatic bacteriuria is increased in women with diabetes. Granulocyte function impairment, however, cannot be the explanation for this finding.
Physical Adversarial Examples for Object Detectors (2018)

Deep neural networks (DNNs) are vulnerable to adversarial examples-maliciously crafted inputs that cause DNNs to make incorrect predictions. Recent work has shown that these attacks generalize to the physical domain, to create perturbations on physical objects that fool image classifiers under a variety of real-world conditions. Such attacks pose a risk to deep learning models used in safety-critical cyber-physical systems. In this work, we extend physical attacks to more challenging object detection models, a broader class of deep learning algorithms widely used to detect and label multiple objects within a scene. Improving upon a previous physical attack on image classifiers, we create perturbed physical objects that are either ignored or mislabeled by object detection models. We implement a Disappearance Attack, in which we cause a Stop sign to "disappear" according to the detector-either by covering thesign with an adversarial Stop sign poster, or by adding adversarial stickers onto the sign. In a video recorded in a controlled lab environment, the state-of-the-art YOLOv2 detector failed to recognize these adversarial Stop signs in over 85% of the video frames. In an outdoor experiment, YOLO was fooled by the poster and sticker attacks in 72.5% and 63.5% of the video frames respectively. We also use Faster R-CNN, a different object detection model, to demonstrate the transferability of our adversarial perturbations. The created poster perturbation is able to fool Faster R-CNN in 85.9% of the video frames in a controlled lab environment, and 40.2% of the video frames in an outdoor environment. Finally, we present preliminary results with a new Creation Attack, where in innocuous physical stickers fool a model into detecting nonexistent objects.
Rapid detection of sequence variations using polymers of specific oligonucleotides (1992)

Journal Article Rapid detection of sequence variations using polymers of specific oligonucleotides Get access William A. Rudert, William A. Rudert Division of Immunogenetics, Department of Pediatrics, University of Pittsburgh School of Medicine, Rangos Research Center, Children's Hospital of PittsburghPittsburgh, PA 15213, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Massimo Trucco Massimo Trucco Division of Immunogenetics, Department of Pediatrics, University of Pittsburgh School of Medicine, Rangos Research Center, Children's Hospital of PittsburghPittsburgh, PA 15213, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 20, Issue 5, 11 March 1992, Page 1146, https://doi.org/10.1093/nar/20.5.1146 Published: 11 March 1992 Article history Accepted: 23 January 1992 Published: 11 March 1992
Scaleable Purification of Adenovirus Vectors (2008)

Adenovirus vectors currently are being evaluated in gene delivery studies ranging from prophylactic vaccination to therapeutic gene therapy. The quantity of purified virus required for these studies necessitate that purification methods must shift from classical density gradient ultracentrifugation to scaleable approaches. A methodology is described herein using batch centrifugation, tangential flow ultrafiltration, and chromatography to purify adenovirus particles at a scale of approximately 10(13) viral particles. This method has been demonstrated to easily scale an additional 40-fold. While purification of human adenovirus type 5 is exemplified, modifications are suggested for the purification of other serotypes.
Long-term outcomes of responders in a randomized, controlled phase II trial of aptamer AS1411 in AML. (2010)

6557 Background: AS1411 is the most advanced aptamer in oncology clinical development. It targets nucleolin, a protein upregulated on the surface of cancer cells. Data from a phase II study of AS1411 in relapsed and refractory AML were reported at ASCO 2009. In this update, we report follow-up data for the responders in the study. Methods: This randomized, multicenter phase II trial compared AS1411 plus high-dose cytarabine (HiDAC) to HiDAC alone as treatment for relapsed (≤3 previous lines of therapy) or refractory AML. Patients in cohort I were randomized 2:1 to receive AS1411 10 mg/kg/day IV CI days 1-7 + HiDAC 1.5 g/m2 bid days 4-7 (AS1411-10), or HiDAC alone for 4 days (control). Following safety assessment, a second cohort was randomized to receive AS1411 40 mg/kg/day + HiDAC (AS1411-40) or HiDAC alone. Objectives were comparison of response rates (CR+CRp), safety and tolerability between groups. For this analysis, we collected data on post-remission therapy (PRT) and overall survival (OS) among responders. Results: 71 patients were randomized: 22 to AS1411-10, 26 to AS1411-40 and 23 to control. 67 patients were evaluable for safety (AS1411-10, 21; AS1411 40, 25; control, 21). Grade 3 and 4 toxicities were similar across all groups: febrile neutropenia, neutropenia, thrombocytopenia, and infections. Deaths within 28 days of treatment were: AS1411-10, 1/21; AS1411-40, 2/25; and control, 3/21. 59 patients were evaluable for response; AS1411-10, 21% (4CR/19); AS1411-40, 19% (2CR+2CRp/21); and control, 5% (1CRp/19). PRT and OS data for responding patients are tabulated. Conclusions: This phase II trial suggested that addition of AS1411 to cytarabine may enhance anti-leukemic activity and that the combination has an acceptable safety profile in patients with relapsed and refractory AML. Follow-up suggests substantial survival durations in some patients responding to AS1411 + cytarabine. A phase IIb study is now evaluating responses, duration of responses and survival in AML patients randomized to AS1411 + cytarabine or cytarabine alone. Group CR/CRp PRT OS (months) Control CRp None 6 AS1411-10 CR Clinical trial 12 CR HiDAC 12+ CR HSCT 18+ CR HSCT 20+ AS1411-40 CRp Allo SCT 3 CR DLIs, Depocyt 5 CRp - 14+ CR - - Author Disclosure Employment or Leadership Position Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Antisoma Antisoma Antisoma Antisoma
[Retracted] Multi‐Agent Systems in Control Engineering: A Survey (2009)

This paper presents a survey on multi‐agent system (MAS) capabilities in control engineering applications. It describes essential concepts of multi‐agent systems that are related to the control systems and presents an overview on the most important control engineering issues which MAS can be explored. Most important technical aspects in MAS implementation and development in engineering environment are also explained. Design methodologies, standards, tools, and supporting technologies to provide an effective MAS‐based control design are addressed and a discussion on important related standards and protocols is given. Finally, some comments and new perspectives for design and implementation of agent‐based control systems are presented.
Cytokine release syndrome after blinatumomab treatment related to abnormal macrophage activation and ameliorated with cytokine-directed therapy (2013)

Blinatumomab is a CD19/CD3-bispecific T-cell receptor-engaging (BiTE) antibody with efficacy in refractory B-precursor acute lymphoblastic leukemia. Some patients treated with blinatumomab and other T cell-activating therapies develop cytokine release syndrome (CRS). We hypothesized that patients with more severe toxicity may experience abnormal macrophage activation triggered by the release of cytokines by T-cell receptor-activated cytotoxic T cells engaged by BiTE antibodies and leading to hemophagocytic lymphohistiocytosis (HLH). We prospectively monitored a patient during blinatumomab treatment and observed that he developed HLH. He became ill 36 hours into the infusion with fever, respiratory failure, and circulatory collapse. He developed hyperferritinemia, cytopenias, hypofibrinogenemia, and a cytokine profile diagnostic for HLH. The HLH continued to progress after discontinuation of blinatumomab; however, he had rapid improvement after IL-6 receptor-directed therapy with tocilizumab. Patients treated with T cell-activating therapies, including blinatumomab, should be monitored for HLH, and cytokine-directed therapy may be considered in cases of life-threatening CRS. This trial was registered at www.clinicaltrials.gov as #NCT00103285.
Ultra-Small RLID Chip Technology (2006)

Ultra-small radio frequency identification (RFID) chip with its antenna technology is described. The key techniques used in this ultra-small chip to reduce chip size and cost are embedded antenna, double-surface electrode and silicon on insulator (SOI).
Linear ablation in addition to circumferential pulmonary vein isolation (Dallas lesion set) does not improve clinical outcome in patients with paroxysmal atrial fibrillation: a prospective randomized study (2014)

Linear ablation in addition to CPVI (catheter Dallas lesion) did not improve clinical outcomes of RFCA in paroxysmal AF patients and required longer procedure times.
P-selectin is acylated with palmitic acid and stearic acid at cysteine 766 through a thioester linkage (1993)

We report that the adhesion receptor P-selectin can be metabolically labeled with [3H]palmitic acid in human platelets. Analysis of alkaline methanolysis products from labeled protein demonstrated that the radioactivity associated with P-selectin was covalently bound palmitic acid. [3H]Palmitic acid was cleaved by hydroxylamine treatment at neutral pH and by reducing agents, indicating that acylation occurred through a thioester linkage. Both stearic acid and palmitic acid were detected by gas chromatography-mass spectrometry analysis of alkaline hydrolysates of purified P-selectin. Deletion or mutation of Cys766 eliminated [3H] palmitic acid labeling of P-selectin in transfected COS-7 cells. We conclude that the cytoplasmic domain of P-selectin is acylated at Cys766 through a thioester bond. Fatty acid acylation may regulate intracellular trafficking or other functions of P-selectin.
The rise of PAMPA (2005)

The parallel artificial membrane permeability assay (PAMPA), as a passive-permeability screen, is a possible low-cost alternative to cellular models for the earliest ADME primary screening of research compounds. Its popularity in the industry has risen rapidly. This review examines state-of-the-art PAMPA methods. The various covered topics include: different lipid formulations, the quantitative relationships between hexadecane, dioyleyoylphosphatidycholine and Double-Sink PAMPA measurements, the use of individual-well stirring, issues of ultraviolet sensitivity, timing strategies, reproducibility of measurements, the correct pH to perform the measurement to avoid aqueous boundary layer problems, the pKa(flux) method for determining intrinsic permeability coefficients and the cosolvent method for very insoluble molecules. Examples of the determination of permeability of very difficult molecules, but molecules that are well absorbed, are given. Carefully gathered evidence in support of the use of the Double-Sink PAMPA model is presented. The review concludes with a binning strategy to predict human intestinal absorption, based on the use of the sum of permeability coefficients, measured at gradient pH 5.0, 6.2 and 7.4. Opinions regarding the future of PAMPA are offered.