gebhart/patrepeval huggingface dataset:
Package Stress Monitor to Compensate for the Piezo-Hall Effect in CMOS Hall Sensors (2013)

Package-induced stress changes the sensitivity of planar Hall plates via the piezo-Hall effect. In this paper, we present a novel stress sensor that allows us to compensate for this undesired mechanical cross-sensitivity. The new CMOS-compatible sensor is based on a Wheatstone bridge built of eight appropriately arranged n- and p-doped piezoresistors. The differential output signal <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex Notation="TeX">$V_{\rm bridge}$</tex></formula> of the sensor is proportional to the sum of in-plane normal stresses <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\sigma_{xx}+\sigma_{yy}$</tex></formula> with the sensitivity <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$S_{{\rm bridge},\sigma}=\partial\left(V_{\rm bridge}/V_{\rm bias}\right)/\partial\left(\sigma_{xx}+\sigma_{yy}\right)=-0.047~{\rm GPa}^{-1}$</tex></formula> . Doping concentrations larger than <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$10^{19}~{\rm cm}^{-3}$</tex></formula> for both the n- and p-type resistors are used to minimize the parasitic resistor mismatch due to the junction field effect. Simultaneously, the highly doped resistors keep the relative thermal cross-sensitivity of the sensor as small as <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$74~{\rm ppm~K}^{-1}$</tex></formula> . In contrast to conventional sensor rosettes, the new sensor has the advantage of offering a differential output signal. The measured signal is successfully used to decrease the stress-impact on the sensitivity of CMOS Hall sensors by a factor of five.
Normal bone marrow signal-transduction profiles: a requisite for enhanced detection of signaling dysregulations in AML (2011)

Molecular and cytogenetic alterations are involved in virtually every facet of acute myeloid leukemia (AML), including dysregulation of major signal-transduction pathways. The present study examines 5 phosphoproteins (pErk, pAkt, pS6, pStat3, and pStat5) in response to 5 cytokine/growth factors (stem cell factor [SCF], Flt-3/Flk-2 ligand [FL], granulocyte/macrophage-colony stimulating factor [GM-CSF], interleukin-3 [IL-3], and granulocyte-CSF [G-CSF]) within 7 immunophenotypically defined populations, spanning progenitor to mature myeloid/myelomonocytic cells in normal bone marrows with further comparison to AML samples. The normal cohort showed pathway-specific responses related to lineage, maturation, and stimulus. Heterogeneous-signaling responses were seen in homogeneous immunophenotypic subsets emphasizing the additive information of signaling. These profiles provided a critical baseline for detection of dysregulated signaling in AML falling into 4 broad categories, viz lack of response, increased activation, altered constitutive expression, and dysregulated response kinetics, easily identified in 10 of 12 AMLs. These studies clearly show robust and reproducible flow cytometry phosphoprotein analyses capable of detecting abnormal signal-transduction responses in AML potentially contributing to definitive reliable identification of abnormal cells. As functional correlates of underlying genetic abnormalities, signal-transduction abnormalities may provide more stable indicators of abnormal cells than immunophenotyping which frequently changes after therapy and disease recurrence.
Interactive cross-language information retrieval (2001)

article Interactive cross-language information retrieval Share on Author: Douglas W. Oard View Profile Authors Info & Claims ACM SIGIR ForumVolume 35Issue 1Spring 2001 pp 1–3https://doi.org/10.1145/948716.948718Online:01 April 2001Publication History 2citation164DownloadsMetricsTotal Citations2Total Downloads164Last 12 Months2Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Bacillus subtilisfunctional genomics: global characterization of the stringent response by proteome and transcriptome analysis (2002)

The stringent response in Bacillus subtilis was characterized by using proteome and transcriptome approaches. Comparison of protein synthesis patterns of wild-type and relA mutant cells cultivated under conditions which provoke the stringent response revealed significant differences. According to their altered synthesis patterns in response to DL-norvaline, proteins were assigned to four distinct classes: (i) negative stringent control, i.e., strongly decreased protein synthesis in the wild type but not in the relA mutant (e.g., r-proteins); (ii) positive stringent control, i.e., induction of protein synthesis in the wild type only (e.g., YvyD and LeuD); (iii) proteins that were induced independently of RelA (e.g., YjcI); and (iv) proteins downregulated independently of RelA (e.g., glycolytic enzymes). Transcriptome studies based on DNA macroarray techniques were used to complement the proteome data, resulting in comparable induction and repression patterns of almost all corresponding genes. However, a comparison of both approaches revealed that only a subset of RelA-dependent genes or proteins was detectable by proteomics, demonstrating that the transcriptome approach allows a more comprehensive global gene expression profile analysis. The present study presents the first comprehensive description of the stringent response of a bacterial species and an almost complete map of protein-encoding genes affected by (p)ppGpp. The negative stringent control concerns reactions typical of growth and reproduction (ribosome synthesis, DNA synthesis, cell wall synthesis, etc.). Negatively controlled unknown y-genes may also code for proteins with a specific function during growth and reproduction (e.g., YlaG). On the other hand, many genes are induced in a RelA-dependent manner, including genes coding for already-known and as-yet-unknown proteins. A passive model is preferred to explain this positive control relying on the redistribution of the RNA polymerase under the influence of (p)ppGpp.
Reduced-Polymer-Loading, High-Temperature Fracturing Fluids by Use of Nanocrosslinkers (2016)

Summary In the quest to discover more natural-gas resources, considerable attention has been devoted to finding and extracting gas locked within tight formations with permeability in the nano- to microdarcy range. The main challenges associated with working in such formations are the intrinsically high-temperature and high-pressure bottom conditions. For formations with bottomhole temperatures at approximately 350–400°F, traditional hydraulic-fracturing fluids that use crosslinked polysaccharide gels, such as guar and its derivatives, are not suitable because of significant polymer breakdown in this temperature range. Fracturing fluids that can work at these temperatures require thermally stable synthetic polymers such as acrylamide-based polymers. However, such polymers have to be used at very-high concentrations to suspend proppants. The high-polymer concentrations make it very difficult to completely degrade at the end of a fracturing operation. As a consequence, formation damage by polymer residue can reduce formation conductivity to gas flow. This paper addresses the shortcomings of the current state-of-the-art high-temperature fracturing fluids and focuses on developing a less-damaging, high-temperature-stable fluid that can be used at temperatures up to 400°F. A laboratory study was conducted with this novel system, which comprises a synthetic acrylamide-based copolymer gelling agent and is capable of being crosslinked with an amine-containing polymer-coated nanosized particulate crosslinker (nanocrosslinker). The laboratory data have demonstrated that the temperature stability of the crosslinked fluid is much better than that of a similar fluid lacking the nanocrosslinker. The nanocrosslinker allows the novel fluid system to operate at significantly lower polymer concentrations (25–45 lbm/1,000 gal) compared with current commercial fluid systems (50–87 lbm/1,000 gal) designed for temperatures from 350 to 400°F. This paper presents results from rheological studies that demonstrate superior crosslinking performance and thermal stability in this temperature range. This fracturing-fluid system has sufficient proppant-carrying viscosity, and allows for efficient cleanup by use of an oxidizer-type breaker. Low polymer loading and little or no polymer residue are anticipated to facilitate efficient cleanup, reduced formation damage, better fluid conductivity, and enhanced production rates. Laboratory results from proppant-pack regained-conductivity tests are also presented.
Effect of a High-Fructose Weight-Maintaining Diet on Lipogenesis and Liver Fat (2015)

Short-term high-fructose intake was associated with increased DNL and liver fat in healthy men fed weight-maintaining diets.
A validated prognostic index predicting response to dexamethasone and diethylstilbestrol in castrate‐resistant prostate cancer (2010)

The easy-to-use prognostic index that the authors developed was able to identify a subgroup of patients with CRPC who had prolonged survival only 1 month after starting DS therapy.
Spatially indirect excitons as primary photoexcitations in conjugated polymers (1994)

We observe competition between picosecond photoinduced absorption and stimulated emission in poly(paraphenylenevinylene) which demonstrates that they derive from two different pathways. We conclude that the photoinduced absorption originates from nonemissive ``spatially indirect'' singlet excitons which are formed with quantum yield as high as 0.9. Previous arguments about maximum possible electroluminescence device efficiencies and about lasers in conjugated polymer films are reexamined in light of the prevalence of this species.
Optimization of Two-Dimensional Signal Constellations in the Presence of Gaussian Noise (1974)

A considerable amount of literature exists on the problem of selecting an efficient set of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">N</tex> digital signals with in-phase and quadrature components for use in a suppressed carrier data transmission system. However, the signal constellation which minimizes the probability of error in Gaussian noise, under an average power constraint, has not been determined when the number of signals is greater than two. In this paper an asymptotic (large signal-to-noise ratio) expression, of the minimum distance type, is derived for the error rate. Using this expression, a gradient-search procedure, which is initiated from several randomly chosen <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">N</tex> -point arrays, converges in each case to a locally optimum constellation. The algorithm incorporates a radial contraction technique to meet the average signal power constraint. The best solutions are described for several values of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">N</tex> and compared with well-known signal formats. As an example, the best locally optimum 16-point constellation shows an advantage of about 0.5 dB in signal-signal-to-noise ratio over quadrature amplitude modulation. The locally optimum constellations are the vertices of a trellis of (almost) equilateral triangles. As <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">N \rightarrow \infty</tex> , it is rigorously proved in the Appendix that the optimum constellations tend toward an equilateral structure, and become uniformly distributed in a circle.
Intestine-specific expression of acyl CoA:diacylglycerol acyltransferase 1 reverses resistance to diet-induced hepatic steatosis and obesity in Dgat1 mice (2010)

Mice deficient in acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), a key enzyme in triacylglycerol (TG) biosynthesis, are resistant to high-fat (HF) diet-induced hepatic steatosis and obesity. DGAT1-deficient (Dgat1−/−) mice have no defect in quantitative absorption of dietary fat; however, they have abnormally high levels of TG stored in the cytoplasm of enterocytes, and they have a reduced postprandial triglyceridemic response. We generated mice expressing DGAT1 only in the intestine (Dgat1IntONLY) to determine whether this phenotype contributes to resistance to HF diet-induced hepatic steatosis and obesity in Dgat1−/− mice. Despite lacking DGAT1 in liver and adipose tissue, we found that Dgat1IntONLY mice are not resistant to HF diet-induced hepatic steatosis or obesity. The results presented demonstrate that intestinal DGAT1 stimulates dietary fat secretion out of enterocytes and that altering this cellular function alters the fate of dietary fat in specific tissues.
CDK9 inhibitors in acute myeloid leukemia (2018)

Current treatment for acute myeloid leukemia (AML) is less than optimal, but increased understanding of disease pathobiology and genomics has led to clinical investigation of novel targeted therapies and rational combinations. Targeting the cyclin-dependent kinase 9 (CDK9) pathway, which is dysregulated in AML, is an attractive approach. Inhibition of CDK9 leads to downregulation of cell survival genes regulated by super enhancers such as MCL-1, MYC, and cyclin D1. As CDK9 inhibitors are nonselective, predictive biomarkers that may help identify patients most likely to respond to CDK9 inhibitors are now being utilized, with the goal of improving efficacy and safety.
Production of recombinant human annexin V by fed-batch cultivation (2014)

We describe a new fed-batch method to produce recombinant human annexin V in large scale, which may expand the commercial utilities for rhANXAV to applications such as in vivo imaging studies.
Single-shot compressed optical-streaking ultra-high-speed photography (2019)

Single-shot ultra-high-speed imaging is of great significance to capture transient phenomena in physics, biology, and chemistry in real time. Existing techniques, however, have a restricted application scope, a low sequence depth, or a limited pixel count. To overcome these limitations, we developed single-shot compressed optical-streaking ultra-high-speed photography (COSUP) with an imaging speed of 1.5 million frames per second, a sequence depth of 500 frames, and an (x,y) pixel count of 0.5 megapixels per frame. COSUP's single-shot ultra-high-speed imaging ability was demonstrated by recording single laser pulses illuminating through transmissive targets and by tracing a fast-moving object. As a universal imaging platform, COSUP is capable of increasing imaging speeds of a wide range of CCD and complementary metal-oxide-semiconductor cameras by four orders of magnitude. We envision COSUP to be applied in widespread applications in biomedicine and materials science.
Oestrogen has no short‐term effect on intestinal strontium absorption in healthy postmenopausal women (1999)

In healthy postmenopausal women, short-term suppletion with exogenous oral oestrogen did not influence intestinal calcium absorption as measured by the strontium absorption test.
IL-15 superagonist/IL-15RαSushi-Fc fusion complex (IL-15SA/IL-15RαSu-Fc; ALT-803) markedly enhances specific subpopulations of NK and memory CD8+ T cells, and mediates potent anti-tumor activity against murine breast and colon carcinomas (2016)

Interleukin (IL)-15-N72D superagonist-complexed with IL-15RαSushi-Fc fusion protein (IL-15SA/IL-15RαSu-Fc; ALT-803) has been reported to exhibit significant anti-tumor activity in murine myeloma, rat bladder cancer, and murine glioblastoma models. In this study, we examined the immunomodulatory and anti-tumor effects of IL-15SA/IL-15RαSu-Fc in tumor-free and highly metastatic tumor-bearing mice. Here, IL-15SA/IL-15RαSu-Fc significantly expanded natural killer (NK) and CD8+ T cells. In examining NK cell subsets, the greatest significant increase was in highly cytotoxic and migrating (CD11b+, CD27hi; high effector) NK cells, leading to enhanced function on a per-cell basis. CD8+ T cell subset analysis determined that IL-15SA/IL-15RαSu-Fc significantly increased IL-15 responding memory (CD122+, CD44+) CD8+ T cells, in particular those having the innate (NKG2D+, PD1-) phenotype. In 4T1 breast tumor-bearing mice, IL-15SA/IL-15RαSu-Fc induced significant anti-tumor activity against spontaneous pulmonary metastases, depending on CD8+ T and NK cells, and resulting in prolonged survival. Similar anti-tumor activity was seen in the experimental pulmonary metastasis model of CT26 colon carcinoma cells, particularly when IL-15SA/IL-15RαSu-Fc was combined with a cocktail of checkpoint inhibitors, anti-CTLA-4 and anti-PD-L1. Altogether, these studies showed for the first time that IL-15SA/IL-15RαSu-Fc (1) promoted the development of high effector NK cells and CD8+ T cell responders of the innate phenotype, (2) enhanced function of NK cells, and (3) played a vital role in reducing tumor metastasis and ultimately survival, especially in combination with checkpoint inhibitors.
A strategy for the quantification of protein polyethylene glycol (PEG) derivatized sites using iTRAQ - eScholarship (2011)

Polyethylene glycol (PEG)-derivatized proteins are important pharmaceuticals and the characterization of their PEGylation patterns are of great importance to regulatory agencies for their approval. Accurate quantification of the PEGylated sites are technically challenging. The PEG moiety attached to the protein is very bulky and hinders protease digestion, with the removal of PEG a succinyl linker (100.01 Da) is still attached and can be used for identification of PEGylated sites. The dePEGylated protein can also be used in comparison to the Native protein to determine the percent occupancy of PEG on lysine. Here, we present an improved mass spectrometry based method using iTRAQ reagents (AB SCIEX) to quantitate ADI, a PEGylated protein. By using LC coupled with tandem mass spectrometry (QTOF), PEGylated lysines are characterized by measuring the ratio of 114: 115 iTRAQ reagent tags, on Native and dePEGylated protein. 24 of 27 lysines were determined to have a succinyl modification. 14 of 27 lysines were quantified to determine their percent occupancy
Analysis of Post-translational CCR8 Modifications and Their Influence on Receptor Activity (2004)

Post-translational modifications of the extracellular portions of receptors located in the cell membrane can contribute to modulating their biological activity. Using a mutagenesis approach in which single or multiple Tyr-to-Phe, Thr-to-Ala, Ser-to-Ala, and Asn-to-Gln substitutions were made at the appropriate positions, we analyzed the sulfation and glycosylation state of the murine CCR8 chemokine receptor, and the way in which these post-translational modifications affect CCR8 activity. A Y14Y15-to-F14F15 CCR8 mutant was less sulfated than the wild-type receptor. An N8-to-Q8 mutant was less glycosylated than wild-type, and a double T10T12-to-A10A12 mutant showed even less glycosylation. We established a flow cytometric analysis with an Fc-fused form of mouse CCL1 to determine precisely the ligand-binding activity of these mutants. Single mutants at amino acid positions 8, 10 or 12 bound CCL1-Fc similarly to wild-type CCR8, whereas the F14F15 double mutant was essentially inactive and the A10A12 double mutant showed about 65% of wild-type ligand-binding activity. Calcium flux activity assays were performed with these mutants, yielding results consistent with those from the ligand binding assays. These data indicate that sulfation at specific positions of the N-terminal domain of mouse CCR8 is critical for its biological activity, whereas glycosylation has a minor influence.
Optimal detection of apoptosis by flow cytometry depends on cell morphology (1993)

Flow cytometry has recently become a choice technique for the quantitative analysis of apoptosis. Monoparametric DNA analysis usually allows identification of apoptotic cells as a "subdiploid" peak. Progression through apoptosis leads to chromatin condensation, nuclear fragmentation and eventually to cell disruption. Thus, a major problem for the flow cytometric analysis of apoptotic populations is discrimination between debris and apoptotic cells. Here we demonstrate that the best parameter on which to make such a distinction is the DNA content, no matter what type of cell is studied. In contrast, discrimination between apoptotic, non-apoptotic cells, and debris is possible on the basis of scattering signals only in few selected cases, depending on the morphology of the intact cells.
The construction of a syntheticEscherichia coli trppromoter and its use in the expression of a synthetic interferon gene (1982)

An 82 base pair DNA fragment has been synthesised which contains the E. coli trp promoter and operator sequences and also encodes the first Shine Dalgarno sequence of the trp operon. This DNA fragment is flanked by EcoRI and ClaI/TaqI cohesive ends and is thus easy to clone, transfer between vector systems and couple to genes to drive their expression. It has been cloned into plasmid pAT153, producing a convenient trp promoter vector. We have also joined the fragment to a synthetic IFN-alpha 1 gene, using synthetic oligonucleotides to generate a completely natural, highly efficient bacterial translation initiation signal on the promoter proximal side of the IFN gene. Plasmids carrying this construction enable E. coli cells to express IFN-alpha 1 almost constitutively and with significantly higher efficiency than from a lacUV5 promoter based system.
ChemInform Abstract: Nb(salan)‐Catalyzed Asymmetric Epoxidation of Allylic Alcohols with Hydrogen Peroxide. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Differential Inhibition of Mollicute Growth: an Approach to Development of Selective Media for Specific Mollicutes (2002)

The energy-generating pathways of Mycoplasma spp. are diverse. Thus, it was predicted that the ability of inhibitors of these pathways to block growth would vary among species. This prediction was tested with 14 Mycoplasma species and potential inhibitors. The greatest differentiation among test species was obtained using fluoride, iodoacetate (IAA), beta-fluoropyruvate (FP), cibacron blue (CB), L-citrulline, and carbonyl cyanide m-chlorophenylhydrazone. A range of other potential inhibitors, including L-arginine analogues, had little inhibitory effect on growth, and D-arginine was shown to be a growth substrate for arginine-hydrolyzing species. Fluoride selectively inhibited the growth of mycoplasmas that were able only to ferment sugars to lactate and/or to hydrolyze arginine. In contrast, IAA was most effective against organic acid-oxidizing species, and L-citrulline inhibited arginine-hydrolyzing species. Mycoplasma verecundum, a species for which energy sources have not been identified, was relatively resistant to FP. Similarly, Acholeplasma laidlawii was distinguished by its CB resistance.