gebhart/patrepeval huggingface dataset:
Calix[n]arene‐Based Glycoclusters: Bioactivity of Thiourea‐Linked Galactose/Lactose Moieties as Inhibitors of Binding of Medically Relevant Lectins to a Glycoprotein and Cell‐Surface Glycoconjugates and Selectivity among Human Adhesion/Growth‐Regulatory Galectins (2008)

Growing insights into the functionality of lectin-carbohydrate interactions are identifying attractive new targets for drug design. As glycan recognition is regulated by the structure of the sugar epitope and also by topological aspects of its presentation, a suitable arrangement of ligands in synthetic glycoclusters has the potential to enhance their avidity and selectivity. If adequately realized, such compounds might find medical applications. This is why we focused on lectins of clinical interest, acting either as a potent biohazard (a toxin from Viscum album L. akin to ricin) or as a factor in tumor progression (human galectins-1, -3, and -4). Using a set of 14 calix[n]arenes (n=4, 6, and 8) with thiourea-linked galactose or lactose moieties, we first ascertained the lectin-binding properties of the derivatized sugar head groups conjugated to the synthetic macrocycles. Despite their high degree of flexibility, the calix[6,8]arenes proved especially effective for the plant AB-toxin, in the solid-phase model system with a single glycoprotein (asialofetuin) and with human tumor cells in vitro. The bioactivity of the calix[n]arenes was also proven for human galectins. Notably, selectivity for the tested tandem-repeat-type galectin-4 among the three subgroups was determined at the level of solid-phase and cell assays, the large flexible macrocycles again figuring prominently as inhibitors. Alternate and cone versions of calix[4]arene with lactose units distinguished between galectins-1 and -4 versus galectin-3 in cell assays. The results thus revealed bioactivity of galactose-/lactose-presenting calix[n]arenes for medically relevant lectins and selectivity within the family of adhesion/growth-regulatory human galectins.
Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction (1994)

A program is described which calculates the thermal stability and the denaturation behaviour of double-stranded DNAs and RNAs up to a length of 1000 base pairs. The algorithm is based on recursive generation of conditional and a priori probabilities for base stacking. Output of the program may be compared directly to experimental results; thus the program may be used to optimize the nucleic acid fragments, the primers and the experimental conditions prior to experiments like polymerase chain reactions, temperature-gradient gel electrophoresis, denaturing-gradient gel electrophoresis and hybridizations. The program is available in three versions; the first version runs interactively on VAXstations producing graphics output directly, the second is implemented as part of the HUSAR package at GENIUSnet, the third runs on any computer producing text output which serves as input to available graphics programs.
Localization, Ion Channel Regulation, and Genetic Interactions during Abscisic Acid Signaling of the Nuclear mRNA Cap-Binding Protein, ABH1 (2002)

Abscisic acid (ABA) regulates developmental processes and abiotic stress responses in plants. We recently characterized a new Arabidopsis mutant, abh1, which shows ABA-hypersensitive regulation of seed germination, stomatal closing, and cytosolic calcium increases in guard cells (V. Hugouvieux, J.M. Kwak, J.I. Schroeder [2001] Cell 106: 477-487). ABH1 encodes the large subunit of a dimeric Arabidopsis mRNA cap-binding complex and in expression profiling experiments was shown to affect mRNA levels of a subset of genes. Here, we show that the dimeric ABH1 and AtCBP20 subunits are ubiquitously expressed. Whole-plant growth phenotypes of abh1 are described and properties of ABH1 in guard cells are further analyzed. Complemented abh1 lines expressing a green fluorescent protein-ABH1 fusion protein demonstrate that ABH1 mainly localizes in guard cell nuclei. Stomatal apertures were smaller in abh1 compared with wild type (WT) when plants were grown at 40% humidity, and similar at 95% humidity. Correlated with stomatal apertures from plants grown at 40% humidity, slow anion channel currents were enhanced and inward potassium channel currents were decreased in abh1 guard cells compared with WT. Gas exchange measurements showed similar primary humidity responses in abh1 and WT, which together with results from abh1/abi1-1 double-mutant analyses suggest that abh1 shows enhanced sensitivity to endogenous ABA. Double-mutant analyses of the ABA-hypersensitive signaling mutants, era1-2 and abh1, showed complex genetic interactions, suggesting that ABH1 and ERA1 do not modulate the same negative regulator in ABA signaling. Mutations in the RNA-binding protein sad1 showed hypersensitive ABA-induced stomatal closing, whereas hyl1 did not affect this response. These data provide evidence for the model that the mRNA-processing proteins ABH1 and SAD1 function as negative regulators in guard cell ABA signaling.
A new method of calibrating the camera nonlinear model (2012)

The aim of camera calibration is to estimate the internal an external parameters of each camera. With these parameters, the vision measurement applications can be achieved. This paper introduced a new method of calibration for camera nonlinear model. The model will explain how the distortion turns out, involved radial bias, and thin prism distortions. The proposed calibration procedure consists of these steps. Firstly, we create a linear model, to calculate the parameters preliminarily which are not so sensitive. That is external parameters and the main point coordinates. Then the nonlinear parameters can be calculated, such as distortion coefficient. With these parameters we modified the world coordinates using the methods of minimizing the re-projection error. Finally, all of the parameters were calculated again with the modified world coordinates. Through reducing the re-projection error, the world coordinates were corrected. Our aim was to reduce cost and improve accuracy. When calibrating a camera, one doesn't have to measure the calibration target so precisely. Real data experiments showed that the method was robust and more accurate.
Low Supply Voltage CMOS Charge Pumps (1997)

Charge pumps that can operate under a low supply voltage are described. The use of charge transfer switches (CTSs) can improve the voltage pumping gain. Applying dynamic control to the CTSs can reduce reverse currents. Appending bootrapped output stages to the new charge pumps can further expand the maximum output voltage.
Fetal and Adult Fibroblasts Have Similar TGF-?????Mediated, Smad-Dependent Signaling Pathways (2006)

Background: The scarless fetal skin-healing mechanism is mediated in part by the fibroblast and involves differential expression of transforming growth factor (TGF)-β isoforms 1 and 3. The authors hypothesized that fetal and adult fibroblasts respond differently to TGF-β isoform-specific stimulation, which may influence whether wounds scar. Connective tissue growth factor (CTGF), Smad3, and Smad7 are TGF-β target genes. Expression of these targets was quantitated after TGF-β1 and -β3 stimulation of fetal and adult fibroblasts. Methods: Primary mouse fibroblast cultures at gestational day 16.5 (E17), 18.5 (E19), and 6 weeks (adult) were stimulated with TGF-β1 or TGF-β3. Quantitative polymerase chain reaction was performed for CTGF, Smad3, and Smad7 expression. Results: CTGF was reduced four-fold in E17 and E19 compared with adult fibroblasts (p < 0.005). After TGF-β1 stimulation, CTGF expression increased more than 60-fold in both E17 and E19 (p < 0.01), which was three-fold greater than that in adult fibroblasts (p < 0.005). TGF-β3 induced more than 70-fold, 50-fold, and 20-fold increases in CTGF expression in E17, E19, and adult fibroblasts, respectively (p < 0.01 for each). Both TGF-β1 and -β3 decreased Smad3 expression and increased Smad7 expression in each fibroblast type, suggesting that intact TGF-β–mediated signaling pathways were present. Conclusions: Fetal (E17 and E19) fibroblasts have lower CTGF expression compared with adult fibroblasts. However, fetal fibroblasts have larger increases in CTGF expression after TGF-β1 or -β3 stimulation. Fetal and adult mouse fibroblasts have similar TGF-β1 and TGF-β3 transcriptional regulation of Smad3 and Smad7. This suggests that scarless healing is likely not mediated by different Smad-dependent transcriptional responses to TGF-β isoforms in the fetal E17 fibroblast.
Dehazing Using Color-Lines (2014)

Photographs of hazy scenes typically suffer having low contrast and offer a limited visibility of the scene. This article describes a new method for single-image dehazing that relies on a generic regularity in natural images where pixels of small image patches typically exhibit a 1D distribution in RGB color space, known as color-lines. We derive a local formation model that explains the color-lines in the context of hazy scenes and use it for recovering the scene transmission based on the lines' offset from the origin. The lack of a dominant color-line inside a patch or its lack of consistency with the formation model allows us to identify and avoid false predictions. Thus, unlike existing approaches that follow their assumptions across the entire image, our algorithm validates its hypotheses and obtains more reliable estimates where possible. In addition, we describe a Markov random field model dedicated to producing complete and regularized transmission maps given noisy and scattered estimates. Unlike traditional field models that consist of local coupling, the new model is augmented with long-range connections between pixels of similar attributes. These connections allow our algorithm to properly resolve the transmission in isolated regions where nearby pixels do not offer relevant information. An extensive evaluation of our method over different types of images and its comparison to state-of-the-art methods over established benchmark images show a consistent improvement in the accuracy of the estimated scene transmission and recovered haze-free radiances.
Asymptotic coverage and rate in massive MIMO networks (2014)

Massive multiple-input multiple-output (MIMO) is a transmission technique for cellular systems that uses many antennas to support not-as-many users. In this paper, we analyze its performance in large-scale cellular networks with Poisson distributed base station locations. Our analysis incorporates the spatial correlation due to small-scale fading at base station antennas. Numerical results show that, though limited by pilot contamination, massive MIMO is still promising to provide high cell throughput by serving large number of users simultaneously.
Polymerization behavior of -olefins with rac- and meso-type ansa-metallocene catalysts: Effects of cocatalyst and metallocene ligand (1999)

Polymerizations of propene, 1-butene and 1-hexene were conducted with a mixture of rac- and meso-[dimethylsilylenebis(2-methylindenyl)]zirconium dichloride (1) combined with methylaluminoxane (MAO), triethylaluminium (AlEt3)/triphenylcarbenium tetrakis(pentafluorophenyl)borate (2) or triisobutylaluminium (AliBu3)/2 as a cocatalyst. The polymerization profiles of propene with rac-1 and meso-1 were determined from the rate of overall propene consumption and the fractions of isotactic and atactic polymers which were sampled during polymerization. An induction time to reach the maximum Rp (rate of polymerization) followed by gradual decay was observed in the case of using the systems rac-, meso-1–MAO and rac-1–AliBu3/2. Besides, a rapid drop of Rp from the initial value was found when using AlEt3/2. Molecular weights of the isotactic and atactic polymers sampled do not change during polymerization, and it is suggested that the change of [C*] (number of active centers) is reflected in the profiles of Rp. The rate ratio of rac-1 to meso-1 (Rp(rac)/Rp(meso)) in propene and 1-butene polymerizations decreases in the following order: AlEt3/2 > AliBu3/2 > MAO. In the case of 1-hexene polymerization, the highest Rp(rac)/Rp(meso) value was obtained. This result indicates that the coordination of 1-hexene to the sterically hindered site of meso-1 is difficult compared with propene and 1-butene.
Substitutional Doping of Carbon Nanotubes with Heteroatoms and Their Chemical Applications (2014)

The electronic properties of carbon nanotubes (CNTs) can be tuned by substitutional doping with heteroatoms (mainly B and N) to expand the applications of CNTs. Based on the comprehensive understanding of the substitutional doping of CNTs, it should be possible to deliberately design doped CNTs for specific purposes. Thus, relevant experimental and theoretical works are reviewed herein in an attempt to correlate the synthetic methods, electronic properties, and applications of heteroatom-doped CNTs. The distribution and arrangement of heteroatoms in the graphitic lattice of CNTs can be modulated through the choice of synthetic conditions, which would further lead to different electronic properties of CNTs for their chemical applications.
Expression of p-STAT3 in human colorectal adenocarcinoma and adenoma; correlation with clinicopathological factors (2005)

This is the first study to report a significant correlation of p-STAT3 expression with the depth of tumour invasion. These findings suggest that p-STAT3 expression is an important factor related to carcinogenesis and/or tumour invasion of colorectal adenocarcinoma.
Detection of picomolar levels of interleukin-8 in human saliva by SPR (2005)

Researchers at UCLA have discovered that the levels of interleukin-8 (IL-8) protein in the saliva of healthy individuals and patients with oropharyngeal squamous cell carcinoma (OSCC) are 30 pM and 86 pM, respectively. In this study, we present the development of the first immunoassay for the quantification of picomolar IL-8 concentrations in human saliva using Biacore surface plasmon resonance (SPR) in a microfluidic channel. A sandwich assay using two monoclonal antibodies, which recognize different epitopes on the antigen (IL-8), was used. Only 13 minutes were required to determine the quantity of pure IL-8 added to just 100 microL of either buffer or saliva-based samples. The limit of detection (LOD) of this immunoassay in buffer was 2.5 pM, and the precision of the response for each concentration was <3% of the coefficient of variation. When first analyzing the saliva supernatants, non-specific binding to the surface was observed. By adding carboxymethyl dextran sodium salt (10 mg mL(-1)) to compete with the surface dextran and primary antibody for non-specific interactions, the signal to noise ratio was greatly improved. The LOD of this immunoassay in saliva was 184 pM. A minimum concentration of 250 pM of exogenous IL-8 could then be consistently detected in a salivary environment. The precision of the response for each IL-8 concentration tested was <7% of the coefficient of variation. Diagnostic sensitivity for oral cancer can be achieved by pre-concentrating the saliva samples 10 fold prior to SPR analysis, making the target levels of IL-8 300 pM for healthy individuals and 860 pM for oral cancer patients.
Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells. (1992)

An androgen-dependent mouse mammary carcinoma cell line (SC-3) requires androgen for growth stimulation. We have shown previously that androgen acts on SC-3 cells to induce secretion of a fibroblast growth factor (FGF)-like growth factor, which in turn stimulates growth of the cells in an autocrine manner. In this study, the androgen-induced growth factor (AIGF) was purified from a conditioned medium of SC-3 cells stimulated with testosterone. cDNA cloning of AIGF by use of its partial amino acid sequence data revealed that AIGF is a distinctive FGF-like growth factor. An AIGF cDNA (pSC17) encodes a 215-amino acid protein with a putative signal peptide, which shares 30-40% homology with known members of the FGF family. The AIGF mRNA was markedly induced by 10 nM testosterone in Northern blot analysis. Expression of AIGF cDNA in mammalian cells clearly showed remarkable stimulatory effects of AIGF on growth of SC-3 cells in the absence of androgen. Thus, it is clear that the androgen-induced growth of SC-3 cells is mediated in an autocrine manner by AIGF, which is secreted by the tumor cells themselves in response to hormonal stimuli.
Increase in Frequency of HPRT Mutation in Tongue Cancer Patients After Radiotherapy: Two Cases (2003)

The mutation frequency of the hypoxanthine-guanine phosphoribosyl transferase gene (HPRT ) in the peripheral T-lymphocytes of two patients treated with radiotherapy (RT) for tongue cancers was studied. The mutation or variant frequency (Vf) at the HPRT locus was assayed by an immunofluorescent staining method to monitor the genotoxic effects of RT. Both patients underwent conventional radical RT to a total tumor dose of 70 Gy, without chemotherapy (CT). The peripheral blood samples were obtained three times from each patientbefore RT (pre-RT), at a cumulative dose of 36-40 Gy (during RT), and immediately after the last irradiation (post-RT). The HPRT variant frequency did not change significantly during RT as compared to that of pre-RT, but increased significantly at post-RT. The Vf of HPRT in these tongue cancer patients post-RT exhibited a three-fold increase over that of pre-RT, implying a risk of genetic mutation for patients treated with RT.
Quantum size effects in the redox potentials, resonance Raman spectra, and electronic spectra of CdS crystallites in aqueous solution (1983)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation R. Rossetti, S. Nakahara, L. E. Brus; Quantum size effects in the redox potentials, resonance Raman spectra, and electronic spectra of CdS crystallites in aqueous solution. J. Chem. Phys. 15 July 1983; 79 (2): 1086–1088. https://doi.org/10.1063/1.445834 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioThe Journal of Chemical Physics Search Advanced Search |Citation Search
A phase I, double-blind, placebo-controlled study of the tolerance and pharmacokinetic behaviour of RP 59500 (1992)

The tolerance and pharmacokinetic behaviour of a new injectable streptogramin antibiotic, RP 59500 were evaluated on 26 healthy male volunteers in a double-blind, placebo-controlled, phase I study. The doses used were 1.4, 2.8, 4.6, 7.0, 9.8, 12.6, 16.8, 22.4 and 29.4 mg/kg; each dose was administered as a 1 h infusion to eight subjects, two of them receiving placebo. Blood levels of RP 59500 were measured by both microbiological and HPLC assays. At the end of the infusion, Cmax for RP 59500 ranged from 0.95 +/- 0.22 to 24.20 +/- 8.82 mg/L. The apparent elimination half-life of the compound ranged from 1.27 to 1.53 h. RP 59500 did not significantly affect any of the laboratory or clinical assessments (blood pressure, pulse rate, ECG, peak expiratory flow rate). Reported adverse effects were of mild intensity; the most frequent event was mild pain or burning at the infusion site with doses of 7 mg/kg or higher. These results support further studies of RP 59500 in phase II clinical trials.
A ferrofluidic magnetic micropump (2001)

A microfluidic pump is described that uses magnetic actuation to push fluid through a microchannel. Operation relies on the use of magnetically-actuated plugs of ferrofluid, a suspension of nanosize ferromagnetic particles. The ferrofluid contacts but is immiscible with the pumped fluid. The prototype circular design demonstrates continuous pumping by regenerating a translating ferrofluidic plug at the conclusion of each pumping cycle. The flow rate can be controlled by adjusting device dimensions or the velocity of an external permanent magnet that directs the motion of the ferrofluid. The ferrofluidic plugs also serve as valves; if the magnetic actuator is stopped, pressure can be maintained with no power consumption. Flow can also be reversed by switching the direction of actuation. The maximum flow rate achieved with minimal backpressure was 45.8 /spl mu/l/min. The maximum pressure head achieved was 135 mm water (1.2 kPa).
The carboxyl terminus of the Bacillus subtilis SecA is dispensable for protein secretion and viability (2000)

The Escherichia coli secretion-dedicated chaperone SecB targets a subset of proteins to the translocase by interacting with the carboxyl (C-) terminus of SecA. This region of SecA is highly conserved in Eubacteria, but despite its presence in the Bacillus subtilis SecA, the B. subtilis genome does not appear to contain a gene for a clear homologue of SecB. Deletion of the C-terminus of the B. subtilis SecA yields cells that have normal viability, but that exhibit a response reminiscent of oxidative stress and the loss of a number of secretory proteins from the culture supernatant. Semi-quantitative RT-PCR demonstrates that these proteins are expressed at lower levels. The C-terminus of SecA fused to glutathione S:-transferase (GST) specifically binds a cytosolic protein, termed MrgA. This protein has been reported to function in relation to oxidative stress, but deletion of the mrgA gene does not result in a secretion defect nor does it cause an oxidative stress response. It is concluded that the C-terminus of the B. subtilis SecA is not essential for secretion and viability.
Methyl jasmonate vapor increases the developmentally controlled synthesis of alkaloids in Catharanthus and Cinchona seedlings (1994)

Summary Shortly after germination, alkaloids are rapidly synthesized in seedlings of both Catharanthus roseus L.G. Don and Cinchona ledgeriana Moens. The effect of low‐level, atmospheric methyl jasmonate on this developmentally controlled process was studied. In both species, about 1 p.p.m. of methyl jasmonate vapor significantly enhanced alkaloid synthesis during germination, resulting in a doubling of alkaloid content in seedlings. Treatment with methyl jasmonate resulted in increased allocation of alkaloid precursors and in enhanced enzyme activities in alkaloid biosynthesis. The ability of methyl jasmonate to increase alkaloid biosynthesis decreased with age of the seedlings. Susceptibility of the process to methyl jasmonate was confined to a narrow time interval where the developmentally regulated onset of alkaloid synthesis occurred. When methyl jasmonate was applied at later developmental stages, its ability to enhance alkaloid content in the seedlings declined sharply.
The Arabidopsis thaliana HY1 locus, required for phytochrome-chromophore biosynthesis, encodes a protein related to heme oxygenases (1999)

The hy1 mutants of Arabidopsis thaliana fail to make the phytochrome-chromophore phytochromobilin and therefore are deficient in a wide range of phytochrome-mediated responses. Because this defect can be rescued by feeding seedlings biliverdin IXalpha, it is likely that the mutations affect an enzyme that converts heme to this phytochromobilin intermediate. By a combination of positional cloning and candidate-gene isolation, we have identified the HY1 gene and found it to be related to cyanobacterial, algal, and animal heme oxygenases. Three independent alleles of hy1 contain DNA lesions within the HY1 coding region, and a genomic sequence spanning the HY1 locus complements the hy1-1 mutation. HY1 is a member of a gene family and is expressed in a variety of A. thaliana tissues. Based on its homology, we propose that HY1 encodes a higher-plant heme oxygenase, designated AtHO1, responsible for catalyzing the reaction that opens the tetrapyrrole ring of heme to generate biliverdin IXalpha.