gebhart/patrepeval huggingface dataset:
Sensitivity of DNA-Mediated Electron Transfer to the Intervening π-Stack:  A Probe for the Integrity of the DNA Base Stack (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSensitivity of DNA-Mediated Electron Transfer to the Intervening π-Stack: A Probe for the Integrity of the DNA Base StackDaniel B. Hall and Jacqueline K. BartonView Author Information Division of Chemistry and Chemical Engineering California Institute of Technology Pasadena, California 91125 Cite this: J. Am. Chem. Soc. 1997, 119, 21, 5045–5046Publication Date (Web):May 28, 1997Publication History Received4 February 1997Published online28 May 1997Published inissue 1 May 1997https://pubs.acs.org/doi/10.1021/ja970366khttps://doi.org/10.1021/ja970366krapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views529Altmetric-Citations141LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Charge transfer,Genetics,Oxidation,Probes,Rhodium Get e-Alerts
Existence of human DAZLA protein in the cytoplasm of human oocytes (1999)

The human deleted in azoospermia-like autosomal (DAZLA) gene is thought to be a candidate gene for azoospermia. cDNA encoding the C-terminal 94 amino acid residues of human DAZLA was used to express a bacterial fusion protein which was then used to raise polyclonal antibodies in rabbits. Immunohistochemical analyses with the human DAZLA antiserum showed that the DAZLA protein is expressed at a cytoplasmic location in female germ cells. Available evidence suggests that the DAZLA gene is a participant in human oogenesis.
Cd40 Ligand (Cd154) Triggers a Short-Term Cd4+ T Cell Activation Response That Results in Secretion of Immunomodulatory Cytokines and Apoptosis (2000)

Signals generated through CD28-B7 and CD40 ligand (CD40L)-CD40 interactions have been shown to be crucial for the induction of long-term allograft survivability. We have recently demonstrated that humanized anti-CD40L (hu5C8) prevents rejection of mismatched renal allografts in primates. To investigate potential mechanisms of CD40L-induced allograft acceptance, we coimmobilized hu5C8 with suboptimal amounts of anti-CD3 to stimulate CD4(+) T cells. We now report that anti-CD3/CD40L costimulation results in CD28-independent activation and subsequent deletion of resting T cells. Coligation of CD3 and CD40L increased expression of CD69, CD25, and CD54 on CD4(+) T cells. We also found that costimulation with anti-CD3/CD40L resulted in enhanced production of interleukin (IL)-10, interferon gamma, and tumor necrosis factor alpha but not IL-2 or IL-6. Interestingly, after several days, anti-CD3/CD40L-mediated activation was followed by apoptosis in a significant population of cells. Consistent with that observation, anti-CD3/CD40L did not enhance the antiapoptotic proteins Bcl-2 and Bcl-xL. Further, the addition of CD28 at 24 h failed to rescue those cells induced to die after costimulation with anti-CD3/CD40L. Together, these data suggest that the graft-sparing effect of hu5C8 in vivo may result in part from early and direct effects on CD4(+) T cells, including a vigorous induction of immunomodulatory cytokines and/or apoptosis of allograft-specific T cells.
Positive allosteric modulators of the μ‐opioid receptor: a novel approach for future pain medications (2014)

This article is part of a themed section on Opioids: New Pathways to Functional Selectivity. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2015.172.issue-2.
Therapeutic vaccination with a trivalent T‐cell receptor (TCR) peptide vaccine restores deficient FoxP3 expression and TCR recognition in subjects with multiple sclerosis (2007)

Therapeutic vaccination using T-cell receptor (TCR) peptides from V genes commonly expressed by potentially pathogenic T cells remains an approach of interest for treatment of multiple sclerosis (MS) and other autoimmune diseases. We developed a trivalent TCR vaccine containing complementarity determining region (CDR) 2 peptides from BV5S2, BV6S5 and BV13S1 emulsified in incomplete Freund's adjuvant that reliably induced high frequencies of TCR-specific T cells. To evaluate induction of regulatory T-cell subtypes, immunological and clinical parameters were followed in 23 treatment-naïve subjects with relapsing-remitting or progressive MS who received 12 monthly injections of the trivalent peptide vaccine over 1 year in an open-label study design. Prior to vaccination, subjects had reduced expression of forkhead box (Fox) P3 message and protein, and reduced recognition of the expressed TCR repertoire by TCR-reactive cells compared with healthy control donors. After three or four injections, most vaccinated MS subjects developed high frequencies of circulating interleukin (IL)-10-secreting T cells specific for the injected TCR peptides and significantly enhanced expression of FoxP3 by regulatory T cells present in both 'native' CD4+ CD25+ and 'inducible' CD4+ CD25- peripheral blood mononuclear cells (PBMC). At the end of the trial, PBMC from vaccinated MS subjects retained or further increased FoxP3 expression levels, exhibited significantly enhanced recognition of the TCR V gene repertoire apparently generated by perturbation of the TCR network, and significantly suppressed neuroantigen but not recall antigen responses. These findings demonstrate that therapeutic vaccination using only three commonly expressed BV gene determinants can induce an expanded immunoregulatory network in vivo that may optimally control complex autoreactive responses that characterize the inflammatory phase of MS.
Operations Research: Applications and Algorithms fourth edition (2004)

Buku teks yang memimpin pasar untuk kursus ini, RISET OPERASI Winston sangat sukses karena orientasi praktisnya dan penekanan yang konsisten pada perumusan model dan pembangunan model. Ini bergerak melampaui studi algoritma belaka tanpa mengorbankan ketelitian yang diinginkan fakultas. Seperti di setiap edisi, Winston memperkuat fitur dan liputan buku yang sukses dengan perkembangan terkini di lapangan. CD-ROM Student Suite, yang sekarang menyertai setiap salinan teks baru, berisi versi terbaru dari perangkat lunak komersial untuk pengoptimalan, simulasi, dan analisis keputusan.
Isolation of an interleukin‐1‐like factor from human joint effusions (1983)

Interleukin-1 (IL-1) is a macrophage derived mediator whose properties suggest that it could play a role in the pathology of arthritis. To test this hypothesis, joint fluids from patients with serveral different arthritides were tested. Small amounts of IL-1-like activity were recovered from many of these joint fluids after affinity chromatography over a column of rabbit anti-human IL-1. Positive fluids were obtained from patients with rheumatoid arthritis, psoriatic arthritis, Reiter's syndrome, osteoarthritis, gout, and traumatic arthritis. Upon gel filtration, the joint derived factor displayed a molecular weight distribution similar to that of IL-1 derived from human monocytes stimulated in vitro. These results suggest that IL-1 is present in joint effusions and, therefore, might contribute to joint destruction.
Efficacy of Galcanezumab in Patients Who Failed to Respond to Preventives Previously: Results from EVOLVE-1, EVOLVE-2 and REGAIN Studies (S20.004) (2018)

To assess if there were any differential treatment effects in patients who had failed ≥2 previous preventives versus those who had not through a subgroup analysis of three Phase 3 studies of galcanezumab.
Antifungal susceptibility testing (1993)

Unlike antibacterial susceptibility testing, reliable antifungal susceptibility testing is still largely in its infancy. Many methods have been described, but they produce widely discrepant results unless such factors as pH, inoculum size, medium formulation, incubation time, and incubation temperature are carefully controlled. Even when laboratories agree upon a common method, interlaboratory agreement may be poor. As a result of numerous collaborative projects carried out both independently and under the aegis of the Subcommittee on Antifungal Susceptibility Testing of the National Committee for Clinical Laboratory Standards, the effects of varying these factors have been extensively studied and a standard method which minimizes interlaboratory variability during the testing of Candida spp. and Cryptococcus neoformans has been proposed. This review summarizes this work, reviews the strengths and weaknesses of the proposed susceptibility testing standard, and identifies directions for future work.
Total synthesis of (-)-rapamycin using an Evans-Tishchenko fragment coupling (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of (-)-rapamycin using an Evans-Tishchenko fragment couplingDaniel Romo, Stephanie D. Meyer, Donna D. Johnson, and Stuart L. SchreiberCite this: J. Am. Chem. Soc. 1993, 115, 17, 7906–7907Publication Date (Print):August 1, 1993Publication History Published online1 May 2002Published inissue 1 August 1993https://pubs.acs.org/doi/10.1021/ja00070a058https://doi.org/10.1021/ja00070a058research-articleACS PublicationsRequest reuse permissionsArticle Views2186Altmetric-Citations102LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
On the Aromatic Stability of a Conjugated C60 Cluster (1987)

The aromatic stability of recentIy reported conjugated sixty--carbon system of spherical shape (representing a truncated icosahedron and named buckminsterful1erene) is discussed.Buckminsterfullerene is predicted by a majority of theoretical methods to be aromatic compound with the degree of aromaticity smaller than that of benzene.
Specific Marker Expression and Cell State of Schwann Cells during Culture In Vitro (2015)

Schwann cells (SCs) in animals exist in different developmental stages or wound repair phases, distinguished mainly by the expression of SC-specific markers. No study has yet determined SC state under in vitro culture conditions, and the specific markers expressed in SC are obscure as well. In this study, we harvested sciatic nerves from newborn mice and isolated SCs by an enzyme-digestion method, then we examined the expression profiles of ten markers (S100, p75NTR, Sox10, Sox2, GAP43, NCAM, Krox20, Oct6, MBP, and MPZ) at both the RNA and protein levels in in vitro mouse SCs and speculated their relation with in vivo SC stages. We assayed RNA and protein levels of SC specific markers by immunofluorescence, Western Blot, and real-time quantitative RT-PCR. The results show that the expression of most markers (S100, p75NTR, GAP43, NCAM, Krox20, Oct6, MBP and MPZ) was not detectable in all of early stage cultured SCs. The expression of transcription factors Sox10 and Sox2 was, however, detectable in all SCs. After 8 days, the positive expression rate of all markers except GAP43 and Oct6 was almost 100%.These results indicates Sox10 is a necessary marker for SC identification, while S100 is not reliable. SCs cultured in vitro express Sox2, P75NTR, NCAM, GAP43, Oct6, and MPZ, suggesting that they are similar to in vivo undifferentiated iSCs or dedifferentiated iSCs after nerve injury.
Structural specializations of α4β7, an integrin that mediates rolling adhesion (2012)

The lymphocyte homing receptor integrin α(4)β(7) is unusual for its ability to mediate both rolling and firm adhesion. α(4)β(1) and α(4)β(7) are targeted by therapeutics approved for multiple sclerosis and Crohn's disease. Here, we show by electron microscopy and crystallography how two therapeutic Fabs, a small molecule (RO0505376), and mucosal adhesion molecule-1 (MAdCAM-1) bind α(4)β(7). A long binding groove at the α(4)-β(7) interface for immunoglobulin superfamily domains differs in shape from integrin pockets that bind Arg-Gly-Asp motifs. RO0505376 mimics an Ile/Leu-Asp motif in α(4) ligands, and orients differently from Arg-Gly-Asp mimics. A novel auxiliary residue at the metal ion-dependent adhesion site in α(4)β(7) is essential for binding to MAdCAM-1 in Mg(2+) yet swings away when RO0505376 binds. A novel intermediate conformation of the α(4)β(7) headpiece binds MAdCAM-1 and supports rolling adhesion. Lack of induction of the open headpiece conformation by ligand binding enables rolling adhesion to persist until integrin activation is signaled.
Somebody's Watching Me? (2015)

Most laptops and personal computers have webcams with LED indicators to notify users when they are recording. Because hackers use surreptitiously captured webcam recordings to extort users, we explored the effectiveness of these indicators under varying circumstances and how they could be improved. We observed that, on average, fewer than half of our participants (45%) noticed the existing indicator during computer-based tasks. When seated in front of the computer performing a paper-based task, only 5% noticed the indicator. We performed a followup experiment to evaluate a new indicator and observed that adding onscreen glyphs had a significant impact on both computer-based and non-computer-based tasks (93% and 59% noticed the new indicator, respectively). We discuss how our results can be integrated into current systems, as well as future ubiquitous computing systems.
Application of Modified Grid-Based Approach for Auto Mesh Generation of IT-Related Products (2006)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Self-consistent modelling of helium discharges: investigation of the role of He2+ions (2001)

Helium glow discharges in the p = 6-60 mbar pressure range have been investigated experimentally and by means of a one-dimensional self-consistent hybrid model. Unlike most of the hybrid models developed previously for noble gas discharges, our model also includes He2+ molecular ions. The electrical parameters of the discharges, recorded in the experiments, as well as the electron temperature for the different discharge conditions (determined spectroscopically) have been used as input data for the hybrid model. Our studies show that He2+ ions are present in the discharge even at 6 mbar with a concentration comparable to that of He+ ions. Conversion of atomic to molecular ions and the associative ionization process are identified as main sources of molecular ions. At low pressures the He2+ ions are principally lost at the electrodes, while at higher pressures the recombination processes become their dominant losses. As the rates of recombination processes are strongly pressure-dependent, the optical emission spectrum changes with increasing pressure and shows significant intensity of He2 molecular bands. The contribution of He2+ ions to the ion current at the cathode as well as their contribution to maintenance of the discharge is found to be around 10%, even at the lowest pressure of 6 mbar, where He2+ formation in most of the discharge models is neglected.
The Expression of Sterigmatocystin and Penicillin Genes in Aspergillus nidulans Is Controlled by veA , a Gene Required for Sexual Development (2003)

Secondary metabolism is commonly associated with morphological development in microorganisms, including fungi. We found that veA, a gene previously shown to control the Aspergillus nidulans sexual/asexual developmental ratio in response to light, also controls secondary metabolism. Specifically, veA regulates the expression of genes implicated in the synthesis of the mycotoxin sterigmatocystin and the antibiotic penicillin. veA is necessary for the expression of the transcription factor aflR, which activates the gene cluster that leads to the production of sterigmatocystin. veA is also necessary for penicillin production. Our results indicated that although veA represses the transcription of the isopenicillin synthetase gene ipnA, it is necessary for the expression of acvA, the key gene in the first step of penicillin biosynthesis, encoding the delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase. With respect to the mechanism of veA in directing morphological development, veA has little effect on the expression of the known sexual transcription factors nsdD and steA. However, we found that veA regulates the expression of the asexual transcription factor brlA by modulating the alpha/beta transcript ratio that controls conidiation.
The neuropsychology of obsessive—compulsive disorder: A review and consideration of clinical implications (1997)

Clinicians have suspected that obsessive-compulsive disorder (OCD) is associated with fundamental information-processing deficits beyond those attributable to mood disturbance alone. Neuropsychological investigations can be divided into four broad areas concerned with general intellectual functioning, an 'under-inclusive' thinking style, performance on tests sensitive to frontal lobe impairment and performance on tests of memory. This body of research is critically reviewed. It is suggested that there is modest evidence for the presence of non-verbal and praxic memory deficits in patients with OCD. These deficits are consistent with contemporary theories of fronto-striatal functioning and may represent the cognitive substrate of doubt-related phenomena such as checking. The demonstration of specific memory impairments in OCD may have significant implications for the revision of existing models of OCD and the development of novel treatment strategies that reduce doubt in compulsive checkers by increasing the distinctiveness of past actions.
Real-time collaborative coding in a web IDE (2011)

This paper describes Collabode, a web-based Java integrated development environment designed to support close, synchronous collaboration between programmers. We examine the problem of collaborative coding in the face of program compilation errors introduced by other users which make collaboration more difficult, and describe an algorithm for error-mediated integration of program code. Concurrent editors see the text of changes made by collaborators, but the errors reported in their view are based only on their own changes. Editors may run the program at any time, using only error-free edits supplied so far, and ignoring incomplete or otherwise error-generating changes. We evaluate this algorithm and interface on recorded data from previous pilot experiments with Collabode, and via a user study with student and professional programmers. We conclude that it offers appreciable benefits over naive continuous synchronization without regard to errors and over manual version control.
BRAF and NRAS Mutations Are Frequent in Nodular Melanoma but Are not Associated with Tumor Cell Proliferation or Patient Survival (2005)

Previous studies have shown frequent mutations in the BRAF (V-raf murine sarcoma viral oncogene homolog B1) or NRAS (neuroblastoma RAS viral [V-ras] oncogene homolog) genes in cutaneous melanoma, but the relationship between these alterations and tumor cell proliferation has not been examined in human melanoma. In our study of 51 primary nodular melanomas and 18 paired metastases, we found mutations in BRAF (codon 600, previously denoted 599) in 15 primary tumors (29%) and eight metastases (44%). The figures for NRAS mutations were 27% and 22%, respectively. Mutations in BRAF and NRAS genes were mutually exclusive in all but one case, and were maintained from primary tumors through their metastases. Mutations, however, were not associated with tumor cell proliferation by Ki-67 expression, tumor thickness, microvessel density, or vascular invasion, and there were no differences in patient survival. Although BRAF and NRAS mutations are likely to be important for the initiation and maintenance of some melanomas, other factors might be more significant for proliferation and prognosis in subgroups of aggressive melanoma.
Merrifield chemistry on electropolymers: protection/(photo)deprotection of amine functions (1998)

S. Morlat-Therias, M. S. Passos, S. K. Ibrahim, T. Le Gall, C. J. Pickett, M. S. Passos and M. Arlete Queiros, Chem. Commun., 1998, 1175 DOI: 10.1039/A802292E
Augmented and sustained plasma concentrations after intramuscular injections of molecular variants and deglycosylated forms of tissue-type plasminogen activators. (1990)

We have previously explored induction of coronary thrombolysis with tissue-type plasminogen activator (t-PA) administered intramuscularly. Absorption-enhancing agents that rendered the approach feasible were identified, but large amounts of activator were required and initial elevations of concentrations in plasma could not be sustained. The present study was designed to determine whether more therapeutically favorable plasma concentrations could be induced by genetically engineering or chemically modifying t-PA to prolong its half-life based on the hypothesis that the ratio of absorption to clearance would be increased. Each of four genetically engineered variants (one variant with growth factor and kringle 1 domains deleted and kringle 2 duplicated, a second variant with a cysteine for Arg substitution in the growth factor domain, a third variant with an additional urokinase kringle inserted, and a fourth variant with the growth factor domain deleted) and enzymatically deglycosylated t-PA exhibited prolonged half-life after bolus intravenous injection in rabbits. Each elicited substantially higher and more sustained elevations in plasma after intramuscular injection in rabbits or dogs with absorption-enhancing agents as compared with wild-type t-PA that were not accompanied by a systemic lytic state. Thus, use of molecular variants of t-PA with prolonged half-lives in the circulation permits induction of augmented and sustained elevations of plasma concentrations after intramuscular injection with absorption-enhancing agents as compared with wild-type t-PA, rendering potentially therapeutic blood levels more attainable with relatively modest amounts of material.