gebhart/patrepeval huggingface dataset:
Real Time Mapping and Dynamic Navigation for Mobile Robots (2007)

This paper discusses the importance, the complexity and the challenges of mapping mobile robot's unknown and dynamic environment, besides the role of sensors and the problems inherited in map building. These issues remain largely an open research problems in developing dynamic navigation systems for mobile robots. The paper presenst the state of the art in map building and localization for mobile robots navigating within unknown environment, and then introduces a solution for the complex problem of autonomous map building and maintenance method with focus on developing an incremental grid based mapping technique that is suitable for real-time obstacle detection and avoidance. In this case, the navigation of mobile robots can be treated as a problem of tracking geometric features that occur naturally in the environment of the robot. The robot maps its environment incrementally using the concept of occupancy grids and the fusion of multiple ultrasonic sensory information while wandering in it and stay away from all obstacles. To ensure real-time operation with limited resources, as well as to promote extensibility, the mapping and obstacle avoidance modules are deployed in parallel and distributed framework. Simulation based experiments has been conducted and illustrated to show the validity of the developed mapping and obstacle avoidance approach.
Elevated flagellin-specific immunoglobulins in Crohn's disease (2004)

Crohn's disease (CD) is driven by seemingly aberrant immune responses directed toward commensal enteric microflora. However, the specific antigens targeted by this immune response remain largely undefined. Herein, we demonstrate that common enteric flagellins are one such target of the CD-associated immune response. Thus flagellin may not only drive acute inflammation via activation of Toll-like receptor 5-mediated gene expression but may also serve as a target of the adaptive immune response that maintains the chronic inflammation characteristic of CD.
Modeling of Trabecular Bone and Lamina Dura Following Selective Alveolar Decortication in Rats (2008)

Selective alveolar decortication induced increased turnover of alveolar spongiosa, and the activity was localized; dramatic escalation of demineralization-remineralization dynamics is the likely biologic mechanism underlying rapid tooth movement following selective alveolar decortication.
Autophagic Cell Death of Malignant Glioma Cells Induced by a Conditionally Replicating Adenovirus (2006)

hTERT-Ad may kill telomerase-positive cancer cells by inducing autophagic cell death.
Essential Bioinformatics (2006)

Essential Bioinformatics is a concise yet comprehensive textbook of bioinformatics, which provides a broad introduction to the entire field. Written specifically for a life science audience, the basics of bioinformatics are explained, followed by discussions of the state-of-the-art computational tools available to solve biological research problems. All key areas of bioinformatics are covered including biological databases, sequence alignment, genes and promoter prediction, molecular phylogenetics, structural bioinformatics, genomics and proteomics. The book emphasizes how computational methods work and compares the strengths and weaknesses of different methods. This balanced yet easily accessible text will be invaluable to students who do not have sophisticated computational backgrounds. Technical details of computational algorithms are explained with a minimum use of mathematical formulae; graphical illustrations are used in their place to aid understanding. The effective synthesis of existing literature as well as in-depth and up-to-date coverage of all key topics in bioinformatics make this an ideal textbook for all bioinformatics courses taken by life science students and for researchers wishing to develop their knowledge of bioinformatics to facilitate their own research.
A comparison of thiosulphate-citrate-bile salts-sucrose (TCBS) agar and thiosulphate-chloride-iodide (TCI) agar for the isolation of Vibrio species from estuarine environments (2003)

This study suggests that TCBS is a superior medium when compared with TCI for the isolation of Vibrio spp. from estuarine waters. Because of the public health risk presented by V. vulnificus, V. parahaemolyticus, V. cholerae and other vibrios, the selection of the most appropriate medium for their isolation is extremely important.
A variable-length category-based n-gram language model (2002)

A language model based on word-category n-grams and ambiguous category membership with n increased selectively to trade compactness for performance is presented. The use of categories leads intrinsically to a compact model with the ability to generalise to unseen word sequences, and diminishes the sparseness of the training data, thereby making larger n feasible. The language model implicitly involves a statistical tagging operation, which may be used explicitly to assign category assignments to untagged text. Experiments on the LOB corpus show the optimal model-building strategy to yield improved results with respect to conventional n-gram methods, and when used as a tagger, the model is seen to perform well in relation to a standard benchmark.
Quantitative J correlation: a new approach for measuring homonuclear three-bond J(HNH.alpha.) coupling constants in 15N-enriched proteins (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative J correlation: a new approach for measuring homonuclear three-bond J(HNH.alpha.) coupling constants in 15N-enriched proteinsGeerten W. Vuister and Ad BaxCite this: J. Am. Chem. Soc. 1993, 115, 17, 7772–7777Publication Date (Print):August 1, 1993Publication History Published online1 May 2002Published inissue 1 August 1993https://pubs.acs.org/doi/10.1021/ja00070a024https://doi.org/10.1021/ja00070a024research-articleACS PublicationsRequest reuse permissionsArticle Views2671Altmetric-Citations832LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Continuous Cultivation of Trypanosoma brucei Blood Stream Forms in a Medium Containing a Low Concentration of Serum Protein without Feeder Cell Layers (1989)

Blood stream forms (BSF) of Trypanosoma brucei brucei GUT at 3.1 were propagated in vitro in the absence of feeder layer cells at 37 C, using a modified Iscove's medium (HMI-18). The medium was supplemented with 0.05 mM bathocuproine sulfonate, 1.5 mM L-cysteine, 1 mM hypoxanthine, 0.2 mM 2-mercaptoethanol, 1 mM sodium pyruvate. 0.16 mM thymidine, and 20% (v/v) Serum Plus (SP) (Hazleton Biologics, Lenexa, Kansas). The latter contained a low level of serum proteins (13 micrograms/ml). Each primary culture was initiated by placing 3.5-4 x 10(6) BSFs isolated from infected mice in a flask containing 5 ml of the medium (HMI-9) supplemented with 10% fetal bovine serum (FBS) and 10% SP. The cultures were maintained by replacing the medium every 24 hr for 5-7 days. During this period, many BSFs died. However, from day 4 onward, long slender BSFs increased in number. On days 5-7, trypanosome suspensions were pooled and cell debris was removed by means of diethylaminoethyl cellulose (DE52) column chromatography. Blood stream forms then were collected by centrifugation, resuspended in fresh medium at 7-9 x 10(5)/ml, and transferred to new flasks. Subcultures were maintained by readjusting the BSF density to 7-9 x 10(5)/ml every 24 hr. Concentrations of FBS were reduced gradually at 5-7-day intervals by alternating the amounts of FBS and SP in HMI-9 with 5% FBS and 15% SP, with 2% FBS and 18% SP, and finally with 20% SP (HMI-18). By this method, 2-3 x 10(6) VSFs/ml were obtained consistently every 24 hr. for more than 80 days.(ABSTRACT TRUNCATED AT 250 WORDS)
Isolation of Three Novel Human Genes Encoding G Protein-Coupled Receptors (1995)

We have cloned and mapped the chromosomal location of three novel human genes encoding G protein-coupled receptors that we have named GPR6, GPR5, and GPR4. The entire coding region for each of these genes was contained on single exons. Gene GPR6 encoded a receptor that shared closest identity (71% in the transmembrane regions) with the human orphan receptor GPR3 and was localized to chromosome 6 (q21-q22.1). Northern blot analysis revealed that GPR6 transcripts were abundant in the human putamen and to a lesser extent in the frontal cortex, hippocampus, and hypothalamus. Gene GPR5 encoded a receptor that most closely resembled the orphan receptor RBS11 (48% in the transmembrane regions) and the MIP 1 alpha/RANTES receptor (45% in the transmembrane regions) and was localized to chromosome 3 (p21.3-p21.1). Gene GPR4 shared identity (40% in the transmembrane regions) with the human platelet-activating factor receptor and was localized to chromosome 19 (q13.2-q13.3).
Consequences of telomerase inhibition and combination treatments for the proliferation of cancer cells. (2003)

Telomerase is expressed in most types of tumor cells but not in most somatic cells, suggesting that telomerase inhibitors may be a powerful new approach to cancer chemotherapy. Here we explore this hypothesis by treating cultured human tumor cells with a 2'-O-methoxyethyl oligonucleotide that binds the telomerase RNA template and acts as a potent inhibitor. Treatment of DU145 (Rb(-), p53(-)) and LNCaP (Rb(+), p53(+)) cells causes telomeres to shorten and cell proliferation to stop. Decreased cell proliferation in culture is not observed immediately but occurs after several weeks and is accompanied by telomere shortening. Antiproliferative effects are more profound for cells growing in soft agar or in colony formation assays, with 90% reduction in the colony-forming ability of LNCaP cells after less than 2 weeks of exposure to the inhibitor. Decreased growth of DU145 and LNCaP tumors and large reductions in prostate-specific antigen levels are also observed in vivo in xenograft models. Short-term treatment of cells with telomerase inhibitors does not increase the effects of standard antiproliferative agents paclitaxel, doxorubicin, etoposide, cisplatin, or carboplatin. Long-term inhibition and telomere shortening sensitize DU145 cells, but not LNCaP cells, to cisplatin or carboplatin. These results demonstrate that methoxyethyl oligomers directed against the template region of telomerase are potent agents and that significant antiproliferative effects can be observed after 2-3 weeks of treatment. Reduced cell proliferation and tumor growth support the hypothesis that telomerase inhibition can make a useful contribution to chemotherapy and should encourage broad testing of telomerase inhibitors.
Design of 5.9 ghz dsrc-based vehicular safety communication (2006)

The automotive industry is moving aggressively in the direction of advanced active safety. Dedicated short-range communication (DSRC) is a key enabling technology for the next generation of communication-based safety applications. One aspect of vehicular safety communication is the routine broadcast of messages among all equipped vehicles. Therefore, channel congestion control and broadcast performance improvement are of particular concern and need to be addressed in the overall protocol design. Furthermore, the explicit multichannel nature of DSRC necessitates a concurrent multichannel operational scheme for safety and non-safety applications. This article provides an overview of DSRC based vehicular safety communications and proposes a coherent set of protocols to address these requirements
Combined syngeneic bone marrow transplantation and immunotherapy of a murine B-cell lymphoma: active immunization with tumor-derived idiotypic immunoglobulin (1990)

Recurrence of the underlying malignancy remains a major cause of treatment failure after autologous bone marrow transplantation (BMT) for patients with lymphoma. In this regard, we have developed an immunotherapeutic approach designed to induce resistance against residual tumor cells persisting after BMT. Previous studies in the model system of 38C13, a lethal B-cell lymphoma of C3H origin, have shown that active immunization with purified tumor-derived surface immunoglobulin (Id), as a tumor-associated antigen, produces resistance to tumor growth. Id immunization of lethally irradiated mice at 3 or 5 weeks after reconstitution with syngeneic bone marrow resulted in significantly prolonged survival after tumor challenge compared with nonspecifically immunized controls. Low levels of idiotype-specific antibody were also demonstrated in the sera of specifically immunized mice at this early time, when other functional studies in the literature of immunocompetence after syngeneic reconstitution might have predicted incomplete recovery. Immunization of mice before lethal irradiation and syngeneic marrow reconstitution also induced significant resistance to tumor challenge, suggesting the persistence of established host antitumor immunity through total body irradiation. These studies demonstrate the feasibility of id immunization in conjunction with bone marrow transplantation.
3,5,3'-Triiodothyronine Effects on the Growth Rate and Cell Cycle of Cultured GC Cells* (1981)

The effect of T3 on cell growth was studied in cultured GC cells, a thyroid hormone-sensitive rat pituitary cell line that produces GH. Cells grown in modified Joklik's Minimal Essential Medium with 15% normal serum had a mean doubling time of 27.1 ± 2.8 h, and this was not altered by the addition of T3 in concentrations up to 1 × 10-73 M. When hypothyroid (Tx) calf serum (T3, <0.15 × 10-9 M) was substituted for normal serum [medium designed to simulate Tx conditions (Tx medium)], the growth rate was unchanged for 2 days and then decreased. After 4 days in Tx medium, the mean doubling time was 107.5 ± 19.2 h. The addition of T3 (5 × 10-8 M) to the Tx calf serum used in the medium (Tx-T3 medium) resulted in a detectable increase in growth rate after 2 days and thereafter an exponential growth rate similar to that of cells grown continuously in T3-supplemented medium. The respective growth rates in Tx and Tx-T3 media were not altered by the addition of rat GH (500 ng/ml). To define further the factors contributing to the growth response to T3, the duration of the cell cycle stages of GC cells in exponential growth in Tx or Tx-T3 medium was determined by measuring the percentage of labeled mitoses after 15-min exposure to [3H]thymidine. Compared to cells in Tx-T3 medium, cells in Tx medium exhibited an increase of 7.9-fold in the duration of Gi, of 2.2-fold in G2, and of 1.35-fold in the mitotic (M) stage. No change in the duration of the period of DNA synthesis was apparent. Examination of DNA profiles by flow cytofluorometry showed that the decrease in growth rate and prolongation of Gi, G3, and M stages of the cell cycle in Tx medium were not due to cell damage or abnormalities in DNA ploidy. Thus, T3 effected a 3.9-fold increase in growth rate due to a decrease in the G1, G2, and M stages of the cell cycle. The data suggest that growth rate changes occurred in the entire cell population and did not result from the development of degenerated or altered cells under Tx conditions. Cultured GC cells appear to be a good model for examination of thyroidal effects on the growth and development of mammalian cells. (Endocrinology 108: 259, 1981)
<title>Bandwidth skimming: a technique for cost-effective video on demand</title> (1999)

This paper proposes a new technique for on-demand delivery of streaming media. The idea is to hold in reserve, or `skim', a portion of the client reception bandwidth that is sufficiently small that display quality is not impacted significantly, and yet that is nonetheless enough to support substantial reductions in server and network bandwidth through near-optimal hierarchical client stream merging. In this paper we show that this objective is feasible, and we develop practical techniques that achieve it. The results indicate that server and network bandwidth can be reduced to on the order of the logarithm of the number of clients who are viewing the object, using a small `skim' (e.g., 15%) of client reception bandwidth. These low server and network bandwidths are achieved for every media file, while providing immediate service to each client, and without having to pre-load initial portions of the video at each client.
Molecular imaging to select cancer therapy and evaluate treatment response. (2009)

The ability to assay in vivo biologic processes non-invasively and quantitatively makes molecular imaging a particularly attractive tool for clinical trials of new drugs and for clinical cancer practice. This review highlights the emerging application of molecular imaging to cancer drug testing and clinical cancer treatment. The potential roles that imaging can play in the approach to cancer drug trials and clinical treatment are first highlighted, including applications to early drug testing, Phase II and III clinical trials, and clinical practice. The use of molecular imaging to select cancer therapy is then discussed, citing examples where molecular imaging can be used to measure the expression of therapeutic targets and factors mediating therapeutic resistance. The use of imaging to measure early pharmacodynamic changes and subsequent response to cancer treatment is then reviewed. Finally, the need for standardization and reproducible quantitative imaging analysis is reviewed.
A comparative analysis of the time‐dependent antiproliferative effects of daunorubicin and WP631 (2003)

Jurkat T lymphocytes were treated with daunorubicin and WP631, a daunorubicin-based DNA binding agent, in experiments aimed to analyze cellular uptake of these drugs and their effect on cell viability. WP631 was taken up more slowly than daunorubicin, but laser confocal microscopy and spectrofluorometric quantification showed that the drug accumulated in the cells. Despite the slow uptake rate, the antiproliferative capacity of WP631 (measured as IC50 after a 72-h continuous treatment) was greater than that of daunorubicin. The propensities of daunorubicin and WP631 to promote apoptosis were compared. Our results indicate that the major effect of WP631 was a G2/M arrest followed, after about 72 h of treatment, by polyploidy and mitotic (reproductive) death. In contrast, daunorubicin induced a rapid response with classic features of apoptosis.
Habituation: effects of regular and stochastic stimulation (1972)

The different effects of regular and stochastic stimulation on the flexor reflex of patients with the spinal cord totally divided and of normal subjects and on the blink reflex of normal subjects were studied.When the cutaneous stimulus was above but less than twice threshold, with regular stimulation habituation occurred; with stochastic stimulation, habitutaion either did not occur or it was minimal. Stochastic stimulation was at random stimulus intervals or with random stimulus intensities.When stimulus intensity was around threshold, habituation occurred with both kinds of stimulation, the response to stimulation soon ceasing to occur. When stimulus intensity was greater than twice threshold, habituation did not occur with both kinds of stimulation.When regular stimulation was given after the response had ceased to occur, extinction of the response beyond zero occurred.When a dishabituating stimulus was repeatedly applied in a regular manner, habituation to the dishabituating stimulus occurred.With regular and stochastic stimulation given as conditioning and test series, it was seen that stochastic stimulation caused, in addition to the short-term excitatory effect, the same long-term cumulative depressing effect on excitability as did regular stimulation.
Alternative Splicing of Intron 3 of the Serine/Arginine-rich Protein 9G8 Gene (2001)

9G8 protein belongs to the conserved serine/arginine-rich (SR) protein family, whose members exhibit multiple functions in constitutive and alternative splicing. We have previously shown that 9G8 primary transcripts are subjected to alternative splicing by excision/retention of intron 3 and to a tissue specific modulation. Because both 5'- and 3'-splice sites of intron 3 appear to be suboptimal in vertebrates, we tested the 9G8 intron 3 as a novel model system of alternative splicing. By using an in vitro approach and a mutational analysis, we have identified two purine-rich exonic splicing enhancers (ESE) located in exon 4 and a (GAA)(3) enhancer located in exon 3. These elements act in concert to promote efficient splicing activation both in vitro and in vivo. Titration experiments with an excess of exonic enhancers or SR-specific RNA targets strongly suggest that SR proteins are specifically involved in the activation process. Although ASF/SF2 was expected to interact the most efficiently with ESE according to the enhancer sequences, UV cross-linking coupled or not to immunopurification demonstrates that 9G8 is highly recruited by the three ESE, followed by SC35. In contrast, ASF/SF2 only binds significantly to the (GAA)(3) motif. S100 complementation experiments with individual SR proteins demonstrate that only 9G8 is able to fully restore splicing of intron 3. These results, and the fact that the exon 3 and 4 ESE sequences are conserved in vertebrates, strongly suggest that the alternative splicing of intron 3 represents an important step in the regulation of the expression of 9G8.
Ectopic expression of the knox homeo box gene rough sheath1 alters cell fate in the maize leaf. (1995)

Rough sheath1 (Rs1) is a dominant mutation that alters cell fate and causes unregulated cell division and expansion in the maize leaf. A knox (Kn1 like-homeo box gene) sequence closely linked to the Rs1-O mutation was cloned and shown by transposon mutagenesis to encode the rs1 gene. The deduced amino acid sequence of the RS1 protein is highly similar to KN1 in the homeo domain but contains a unique amino-terminal region. rs1 is expressed in the shoot apical meristem in a circular pattern preceding leaf initiation, but is not detectable in leaf primordia or mature leaves in normal plants. Rings of rs1 expression subtend leaf insertion sites in the shoot, and lateral organ primordia in inflorescence and floral meristems. The timing and position of rs1 expression in meristems suggests a possible role for rs1 in patterning the placement of lateral organs along the axis of the shoot. In contrast to wild type, rs1 is expressed in early leaf primordia of Rs1 mutant plants, suggesting that ectopic expression causes the mutant phenotype. Ectopic expression in Rs1-O plants suggests the ligular [corrected] region is more competent to respond to rs1 than blade or sheath tissues.