gebhart/patrepeval huggingface dataset:
Application of IFDMA to mobile radio transmission (2002)

"Interleaved frequency-division multiple-access" (IFDMA) has been introduced which is a new spread-spectrum multiple-access scheme showing several advantages over other multiple-access schemes. In this paper, the application of IFDMA to mobile radio transmission is considered. Especially, the frequency-diversity achievable with IFDMA taking into account the influence of pulse shaping is investigated. As a result, maximum diversity can be achieved with a set of IFDMA parameters which enables maximum-likelihood detection at the receiver. Therefore, maximum-likelihood detection is proposed for IFDMA and investigated using Monte-Carlo simulations. In comparison with multicarrier code-division multiple-access, IFDMA shows an even better performance whereas having additional advantages, such as avoiding large crest factors.
Relative end-effector control using Cartesian position based visual servoing (1996)

This paper presents a complete design methodology for Cartesian position based visual servo control for robots with a single camera mounted at the end-effector. Position based visual servo control requires the explicit calculation of the relative position and orientation (POSE) of the workpiece object with respect to the camera. This is accomplished using image plane measurements of a number of known feature points on the object, and then applying an extended Kalman filter to obtain a recursive solution of the photogrammetric equations, and to properly combine redundant measurements. The control is then designed by specifying the desired trajectories with respect to the object and forming the control error in the end-effector frame. The implementation using a distributed computer architecture is described. An experimental system has been built and used to evaluate the performance of the POSE estimation and the position based visual servo control. Several results for relative trajectory control and target tracking are presented. Results of the experiments showing the effect of loss of some of the redundant features are also presented.
Identification, Synthesis, and Enzymology of Non‐natural Glucosinolate Chemopreventive Candidates (2008)

Isothiocyanates (ITCs) are one of the many classes of breakdown products of glucosinolates found in crucifers such as broccoli and are thought to be partially responsible for the reduced risk of degenerative diseases associated with the consumption of vegetables. The production of ITCs such as L-sulforaphane is dependent on the hydrolytic bioactivities of myrosinase, localized both within vegetable tissues and within flora of the human GI tract, and is associated with important cancer chemopreventive activities. We hypothesized that novel isothiocyanates with enhanced chemopreventive properties relative to L-sulforaphane could be identified and that their glucosinolate precursors could be synthesized. From a library of 30 synthetic ITCs, we identified several with bioactivities equal or superior to those of L-sulforaphane. The corresponding non-natural glucosinolate precursors to these novel ITCs were constructed and found to be substrates for myrosinase. By utilizing a novel RP-HPLC assay to monitor myrosinase-dependent hydrolysis reactions, 2,2-diphenylethyl glucosinolate and (biphenyl-2-yl)methyl glucosinolate were shown to exhibit 26.5 and 2.8 %, respectively, of the relative activity of sinigrin and produced their corresponding ITCs in varying yields. These data support the notion that non-natural glucosinolates can act as prodrugs for novel ITCs, with a mechanism of action reliant on their hydrolytic cleavage by myrosinase. Such non-natural glucosinolates may serve as very economical chemopreventive agents for individuals at risk for cancers of and around the GI tract.
Differential Phosphorylation of c-Abl in Cell Cycle Determined by cdc 2 Kinase and Phosphatase Activity (1990)

The product of the c-abl proto-oncogene (c-Abl) is phosphorylated on three sites during interphase and seven additional sites during mitosis. Two interphase and all mitotic c-Abl sites are phosphorylated by cdc2 kinase isolated from either interphase or mitotic cells, with the mitotic cdc2 having an 11-fold higher activity. Inhibition of phosphatases with okadaic acid in interphase cells leads to the phosphorylation of c-Abl mitotic sites, indicating that those sites are preferentially dephosphorylated during interphase. The differential phosphorylation of c-Abl in the cell cycle is therefore determined by an equilibrium between cdc2 kinase and protein phosphatase activities. Treatment of interphase cells with okadaic acid leads to a rounded morphology similar to that observed during mitosis.
Phosphorylation of serine residues is fundamental for the calcium‐binding ability of Orchestin, a soluble matrix protein from crustacean calcium storage structures (2003)

Orchestia cavimana is a terrestrial crustacean, which cyclically stores calcium in diverticula of the midgut, in the form of calcified amorphous concretions. These concretions are associated with a proteinaceous matrix, the main constituent of the soluble matrix is Orchestin, an acidic calcium-binding protein [Testenière et al., Biochem. J. 361 (2002) 327-335]. In the present paper, we clearly demonstrate that Orchestin is phosphorylated on serine and tyrosine residues, but that calcium binding only occurs via the phosphoserine residues. To our knowledge, this is the first example of an invertebrate mineralization for which a post-translational modification is clearly related to an important function of a calcifying protein.
Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155 (2006)

Vector-based RNA interference (RNAi) has emerged as a valuable tool for analysis of gene function. We have developed new RNA polymerase II expression vectors for RNAi, designated SIBR vectors, based upon the non-coding RNA BIC. BIC contains the miR-155 microRNA (miRNA) precursor, and we find that expression of a short region of the third exon of mouse BIC is sufficient to produce miR-155 in mammalian cells. The SIBR vectors use a modified miR-155 precursor stem-loop and flanking BIC sequences to express synthetic miRNAs complementary to target RNAs. Like RNA polymerase III driven short hairpin RNA vectors, the SIBR vectors efficiently reduce target mRNA and protein expression. The synthetic miRNAs can be expressed from an intron, allowing coexpression of a marker or other protein with the miRNAs. In addition, intronic expression of a synthetic miRNA from a two intron vector enhances RNAi. A SIBR vector can express two different miRNAs from a single transcript for effective inhibition of two different target mRNAs. Furthermore, at least eight tandem copies of a synthetic miRNA can be expressed in a polycistronic transcript to increase the inhibition of a target RNA. The SIBR vectors are flexible tools for a variety of RNAi applications.
Functional Fe3O4/TiO2 Core/Shell Magnetic Nanoparticles as Photokilling Agents for Pathogenic Bacteria (2008)

A photokilling approach for pathogenic bacteria is demonstrated using a new type of magnetic nanoprobe as the photokilling agent. In addition to their magnetic property, the nanoprobes have other features including a photocatalytic property and the capacity to target bacteria. The nanoprobes comprise iron oxide/titania (Fe(3)O(4)@TiO(2)) core/shell magnetic nanoparticles. As dopamine molecules can self-assemble onto the surface of the titania substrate, dopamine is used as the linker to immobilize succinic anhydride onto the surfaces of the Fe(3)O(4)@TiO(2) nanoparticles. This is followed by the immobilization of IgG via amide bonding. We demonstrate that the IgG-Fe(3)O(4)@TiO(2) magnetic nanoparticles not only have the capacity to target several pathogenic bacteria, but they also can effectively inhibit the cell growth of the bacteria targeted by the nanoparticles under irradiation of a low-power UV lamp within a short period. Staphylococcus saprophyticus, Streptococcus pyogenes, and antibiotic-resistant bacterial strains, such as multiantibiotic-resistant S. pyogenes and methicillin-resistant Staphylococcus aureus (MRSA), are used to demonstrate the feasibility of this approach.
Biodegradable polymer microneedles: fabrication, mechanics and transdermal drug delivery (2005)

To overcome skin's barrier properties that block transdermal delivery of most drugs, we and others have microfabricated arrays of microscopic needles, primarily out of silicon or metal. This study addresses microneedles made of biocompatible and biodegradable polymers, which are expected to improve safety and manufacturability. To make biodegradable polymer microneedles with sharp tips, we adapted microelectromechanical masking and etching to produce beveled-tip and chisel tip microneedles and developed a new fabrication method to produce tapered-cone microneedles using an in-situ lens-based lithographic approach. To replicate microfabricated master structures, PDMS micromolds were generated and a novel vacuum-based method was developed to fill the molds with polylactic acid, polyglycolic acid and their copolymers. Mechanical testing of the resulting needles measured the force at which needles broke during axial loading and found that this failure force increased with Young's modulus of the material and needle base diameter and decreased with needle length. Failure forces were generally much larger than the forces needed to insert microneedles into skin, indicating that biodegradable polymers can have satisfactory mechanical properties for microneedles. Finally, arrays of polymer microneedles were shown to increase permeability of human cadaver skin to a low-molecular weight tracer, calcein, and a macromolecular protein, bovine serum albumin, by up to three orders of magnitude. Altogether, these results indicate that biodegradable polymer microneedles can be fabricated with an appropriate geometry and sufficient strength to insert into skin, and thereby dramatically increase transdermal transport of molecules.
Pomegranate (2007)

Research Article| August 01 2007 Pomegranate Meghan Adler Meghan Adler Search for other works by this author on: This Site PubMed Google Scholar Gastronomica (2007) 7 (3): 10. https://doi.org/10.1525/gfc.2007.7.3.10 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Meghan Adler; Pomegranate. Gastronomica 1 August 2007; 7 (3): 10. doi: https://doi.org/10.1525/gfc.2007.7.3.10 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentGastronomica Search This content is only available via PDF. ©© 2007 BY THE REGENTS OF THE UNIVERSITY OF CALIFORNIA. ALL RIGHTS RESERVED.2007 Article PDF first page preview Close Modal You do not currently have access to this content.
The Polycomb -group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1 (2003)

The Polycomb-group (PcG) proteins MEDEA, FERTILIZATION INDEPENDENT ENDOSPERM, and FERTILIZATION INDEPENDENT SEED2 regulate seed development in Arabidopsis by controlling embryo and endosperm proliferation. All three of these FIS-class proteins are likely subunits of a multiprotein PcG complex, which epigenetically regulates downstream target genes that were previously unknown. Here we show that the MADS-box gene PHERES1 (PHE1) is commonly deregulated in the fis-class mutants. PHE1 belongs to the evolutionarily ancient type I class of MADS-box proteins that have not yet been assigned any function in plants. Both MEDEA and FIE directly associate with the promoter region of PHE1, suggesting that PHE1 expression is epigenetically regulated by PcG proteins. PHE1 is expressed transiently after fertilization in both the embryo and the endosperm; however, it remains up-regulated in the fis mutants, consistent with the proposed function of the FIS genes as transcriptional repressors. Reduced expression levels of PHE1 in medea mutant seeds can suppress medea seed abortion, indicating a key role of PHE1 repression in seed development. PHE1 expression in a hypomethylated medea mutant background resembles the wild-type expression pattern and is associated with rescue of the medea seed-abortion phenotype. In summary, our results demonstrate that seed abortion in the medea mutant is largely mediated by deregulated expression of the type I MADS-box gene PHE1.
ACNE ROSACEA (1933)

Since our recognition several years ago of the presence of Demodex folliculorum in the majority of our patients with acne rosacea and an allied condition which we have designated as pityriasis folliculorum (Demodex), our therapeutic results have been far superior to those obtained by the usual treatment of acne rosacea. In view of the fact that Kaufmann-Wolf1called attention to the frequent occurrence of Demodex folliculorum in the pustules of acne rosacea as long ago as 1925, it seems rather surprising that so little recognition has been accorded this valuable contribution. In fact, within the past few months Stokes and Beerman2have published an exhaustive study of the etiology of acne rosacea especially from an emotional and nervous point of view without making any mention whatever of Demodex as a possible factor. One might argue that, because Demodex is a more or less normal inhabitant of the human
Monoclonal Antibodies to a Synthetic Fibrin-Like Peptide Bind to Human Fibrin But Not Fibrinogen (1983)

A synthetic heptapeptide from the amino terminus of the beta chain in human fibrin was used as an antigen to produce monoclonal antibodies that bind to fibrin even in the presence of human fibrinogen at the concentration found in plasma. As expected, the antifibrin activity was inhibited by the peptide antigen but not by a control heptapeptide. In a chicken ex vivo circulatory model for fibrin detection, intravenously administered monoclonal antibodies bound to human fibrin-coated disks placed in an extracorporeal chamber. These findings may lead to better methods for identifying deep vein and coronary artery thrombi.
VARA's First Five Years (1996)

This article addresses the background, provisions, and brief history of the Visual Artists Rights Act of 1990 (VARA), a federal law protecting the "moral rights" of visual artists. These non-economic rights, which give artists some control over their creations even after the works are sold, have a long history in continental Europe, but are a recent import to the United States. After American courts declined to recognize such rights under common law earlier in this century, approximately a dozen states enacted laws protecting the rights of creators of "fine art." These statutes generally protect the artist's right of integrity-to protect the work from alteration, distortion, or destruction-and the right of attribution-to have her name associated with her work, and not to have her name associated with works she did not create or with distorted versions of her work.\nIn an effort to bring federal uniformity to this area of law and to comply with the Berne Convention, Congress passed VARA, which became effective June 1, 1991. The few litigated cases since VARA's enactment that have resulted in published decisions have raised a number of interesting and difficult questions concerning the operation of the statute. This article discusses those cases and the issues they have raised and resolved-and left unresolved. Those issues include the extent to which VARA preempts analogous state moral rights laws and whether the takings clause is implicated by VARA's provision protecting fine art affixed to real property. Finally, this article discusses the recent report mandated by Congress and produced by the Register of Copyrights analyzing whether the rights created by VARA should be made non-waivable by artists.
Protein ubiquinone interaction. Synthesis and biological properties of 5-alkyl ubiquinone derivatives. (1994)

For the investigation of the protein-ubiquinone interaction in the succinate-cytochrome c reductase region of the bovine heart mitochondrial electron transport chain, a series of 5-alkyl-substituted ubiquinone derivatives (5-R-Q0C10) were synthesized and characterized. Syntheses of 5-ethyl-Q0C10, 5-propyl-Q0C10, 5-isopropyl-Q0C10, and 5-butyl-Q0C10, were archived through radical coupling reactions between 2,3-dimethoxy-6-decyl-1,4-benzoquinone (5-H-Q0C10) and the corresponding alkanoyl peroxides. Although the spectral and redox properties of 5-R-Q0C10 are very similar to those of 5-methyl-2,3 dimethoxy-6-decyl-1,4-benzoquinone, the biological electron transfer efficiencies of these derivatives differ significantly. The reducibility of these derivatives by succinate, as measured with succinate-Q reductase and the oxidizability as measured by ubiquinol-cytochrome c reductase, decreased as the size of the substituents increased. 5-Ethyl-Q0C10 has about 50% of the activity of 5-methyl-2,3-dimethoxy-6-decyl-1,4-benzoquinone, whereas molecules with 5-alkyl groups of three or more carbon atoms are virtually inactive as electron acceptors for succinate-Q reductase. Reduced form of the derivative with no substituent at the 5-position, 5-H derivative is more effectively oxidized by ubiquinol-cytochrome c reductase than does the 5-methyl derivative, the native form. The oxidation of 5-H derivative is in a concentration-dependent manner at low concentrations but exhibits a substrate inhibition at higher concentrations. No such substrate inhibition is observed when other 5-substituted Q derivatives are used. 5-H derivative is a better electron acceptor for succinate-Q reductase than any other Q derivatives and does not show substrate inhibition, even at high concentrations. These results indicate that the binding environment of the benzoquinone ring in succinate-Q reductase is more specific than that of ubiquinol-cytochrome c reductase.
Toward autonomic computing with DB2 universal database (2002)

As the cost of both hardware and software falls due to technological advancements and economies of scale, the cost of ownership for database applications is increasingly dominated by the cost of people to manage them. Databases are growing rapidly in scale and complexity, while skilled database administrators (DBAs) are becoming rarer and more expensive. This paper describes the self-managing or autonomic technology in IBM's DB2 Universal Database® for UNIX and Windows to illustrate how self-managing technology can reduce complexity, helping to reduce the total cost of ownership (TCO) of DBMSs and improve system performance.
Analysis of next-generation genomic data in cancer: accomplishments and challenges (2010)

The application of next-generation sequencing technology has produced a transformation in cancer genomics, generating large data sets that can be analyzed in different ways to answer a multitude of questions about the genomic alterations associated with the disease. Analytical approaches can discover focused mutations such as substitutions and small insertion/deletions, large structural alterations and copy number events. As our capacity to produce such data for multiple cancers of the same type is improving, so are the demands to analyze multiple tumor genomes simultaneously growing. For example, pathway-based analyses that provide the full mutational impact on cellular protein networks and correlation analyses aimed at revealing causal relationships between genomic alterations and clinical presentations are both enabled. As the repertoire of data grows to include mRNA-seq, non-coding RNA-seq and methylation for multiple genomes, our challenge will be to intelligently integrate data types and genomes to produce a coherent picture of the genetic basis of cancer.
Coconut Oil Extraction by a New Enzymatic Process (1986)

ABSTRACT A new method for coconut oil extraction based on the enzymatic action of polygalacturonases, α‐amylase and proteases on a diluted coconut paste was developed. After reaction with enzymes, the solution was centrifuged resulting in three phases: the upper one containing a high quality coconut oil; the middle layer containing water; and the lower phase consisting of coconut meal which has been traditionally used in cattle feed. Eighty percent yields were obtained by this method which was advantageous from the energy point of view compared to the traditional process.
Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus (2009)

The cellular uptake and localization of a Ru-octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru-octaarginine-fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru-octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru-octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru-octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide.
Quantum spinon oscillations in a finite one-dimensional transverse Ising model (2011)

The full quantum dynamics of a spinon under external magnetic fields is investigated by using the time-evolving block decimation (TEBD) method within the microcanonical picture of transport. We show that the center of the spinon oscillates back and forth in the absence of dissipation. The quantum many-body behavior can be understood in a single-particle picture of transport and Bloch oscillations (BOs), where quantum fluctuations induce finite lifetimes. Transport, oscillations, and lifetimes can be tuned to some degree separately by external fields. Other nontrivial dynamics, such as resonance and chaos, are also discussed.
DNA Detection Using Origami Paper Analytical Devices (2013)

We demonstrate the hybridization-induced fluorescence detection of DNA on an origami-based paper analytical device (oPAD). The paper substrate was patterned by wax printing and controlled heating to construct hydrophilic channels and hydrophobic barriers in a three-dimensional fashion. A competitive assay was developed where the analyte, a single-stranded DNA (ssDNA), and a quencher-labeled ssDNA competed for hybridization with a fluorophore-labeled ssDNA probe. Upon hybridization of the analyte with the fluorophore-labeled ssDNA, a linear response of fluorescence vs analyte concentration was observed with an extrapolated limit of detection <5 nM and a sensitivity relative standard deviation as low as 3%. The oPAD setup was also tested against OR/AND logic gates, proving to be successful in both detection systems.
Titanocene‐Catalyzed Cascade Cyclization of Epoxypolyprenes: Straightforward Synthesis of Terpenoids by Free‐Radical Chemistry (2004)

The titanocene-catalyzed cascade cyclization of epoxypolyenes, which are easily prepared from commercially available polyprenoids, has proven to be a useful procedure for the synthesis of C(10), C(15), C(20), and C(30) terpenoids, including monocyclic, bicyclic, and tricyclic natural products. Both theoretical and experimental evidence suggests that this cyclization takes place in a nonconcerted fashion via discrete carbon-centered radicals. Nevertheless, the termination step of the process seems to be subjected to a kind of water-dependent control, which is unusual in free-radical chemistry. The catalytic cycle is based on the use of the novel combination Me(3)SiCl/2,4,6-collidine to regenerate the titanocene catalyst. In practice this procedure has several advantages: it takes place at room temperature under mild conditions compatible with different functional groups, uses inexpensive reagents, and its end step can easily be controlled to give exocyclic double bonds by simply excluding water from the medium.