gebhart/patrepeval huggingface dataset:
Use of a Diode Laser Balloon Ablation Catheter to Generate Circumferential Pulmonary Venous Lesions in an Open‐Thoracotomy Caprine Model (2004)

Electrical isolation of the pulmonary veins (PVs) can be curative in certain patients with atrial fibrillation. The ability of a diode laser balloon ablation catheter to isolate PVs was assessed in an open-thoracotomy caprine model system. After a median sternotomy, the left atrial appendage was cannulated in 19 goats. A laser balloon catheter was placed at the PV ostia and used to deliver photonic energy to the periostial tissue. The applications were delivered at 3.7, 4.5, or 5.4 W/cm for 90-150 seconds. Electrical continuity of the PV with the left atrium was assessed using a multielectrode mapping catheter. After a single application of photonic energy, electrical isolation of the PVs was achieved in (70%) 19/27 PVs. However, the success of electrical PV isolation did not correlate with the dose or duration of the applications. When reflectance spectroscopy was utilized to ensure adequate orientation and contact of the laser balloon catheter with the left atrial myocardium, complete PV isolation was achieved in 5/5 veins at 3.5 W/cm for 120 seconds. Pathological examination revealed no PV stenosis, no pericardial damage, minor lung lesions without pleural perforation, minimal endothelial disruption, and, in the presence of adequate heparinization, no endocardial charring or overlying thrombus. Effective isolation of the PVs can be achieved by delivery of a continuous circular beam of photonic energy to ablate the left atrial - pulmonary venous junction. The use of reflectance spectroscopy to provide real-time monitoring of the blanching effect of balloon-tissue contact optimizes lesion delivery.
Influence of Percent Egg Yolk during Cooling and Freezing on Survival of Bovine Spermatozoa (1979)

The influence of percent egg yolk during cooling from 37 to 5 C on ability of sperm to withstand subsequent freezing was studied with semen from each of eight bulls. Treatments were arranged factorially and included .5, 1, 2, 4, 8, 16, and 32% egg yolk, cooling times of .5, 1, 2, and 4 h, and freezing in ampules and straws. Percent egg yolk did not influence motility or percent intact acrosomes when semen was frozen in ampules. For sperm in straws, both post-thaw percentages of motile sperm and intact acrosomes were similar for sperm cooled with .5, 1, 2, 4, or 8% egg yolk and greater than with 16 or 32% egg yolk. Optimal egg yolk during cooling was not dependent upon rate of cooling, regardless of how semen was packaged for freezing. The influence of percent egg yolk during freezing was investigated in a second study. Treatments included eight bulls; 1, 2, 4, 8, 16, and 32% egg yolk; freezing times from +5 to -130 C of 3.5, 20, and 40 min; and freezing in ampules or straws. Optimal egg yolk during freezing in either ampules or straws was independent of the rate of freezing. For sperm frozen in ampules, differences in motility or the percent intact acrosomes due to egg yolk were small. However, the use of only 1 or 2% egg yolk depressed motility whereas 16 or 32% egg yolk decreased the percentage of intact acrosomes. Thus, extremely high or low egg yolk should be avoided. For sperm in straws, survival was optimal when the extended semen contained 4 to 32% egg yolk during freezing.
Secretion of biologically active murine interleukin-2 by Lactococcus lactis subsp. lactis (1995)

Secretion of functional recombinant murine interleukin-2 (mIL2) by Lactococcus lactis was achieved by fusion of the sequence encoding mature mIL2 to the secretion signal leader of the lactococcal usp45 gene placed under transcriptional control of the phage T7 promoter-T7 RNA polymerase expression system. The recombinant mature mIL2 was one of only a few proteins which accumulated in the growth medium. Sequence analysis revealed correct processing at the first amino acid of the mature protein. A T-cell proliferation assay showed that the recombinant protein has the same specific biological activity as mIL2 obtained from a natural source.
Anorganic Bovine Bone Supports Osteoblastic Cell Attachment and Proliferation (1999)

These results demonstrate that this anorganic bovine bone graft material is able to support the attachment and proliferation of osteoblastic cells.
Motion control of the NTUA robotic snake on a planar surface (2002)

We extend our previous work (1997, 1998) on steering a robotic snake and we now address the problems of (i) stabilization about an arbitrary configuration and (ii) tracking an arbitrary trajectory. For stabilization we apply three different algorithms for the kinematic model derived in the previous work. Comparisons of the three feedback stabilizing controls are made. Trajectory tracking is firstly addressed under the notion of flat systems. In a second approach we make use of a dynamic state feedback in order to achieve dynamic decoupling of our system.
Bilateral Chronic Electrostimulation of Ventroposterolateral Pallidum (1994)

The global improvement of all parkinsonian symptoms after stereotactic pallidotomy has been demonstrated by Leksell. Recently, Laitinen, re-evaluating this target in the neurosurgical treatment of Parkinson's disease, confirmed the real value of this approach, and emphasized the necessity of locating the lesion in the ventroposterolateral part of the pallidum internum. Because we know that high-frequency stimulation of the ventrolateral part of the thalamus has the same clinical effect on tremor as high-frequency coagulation, this technique has now been applied bilaterally in one session in three patients who have severe Parkinson's disease, with akinesia and levodopa-induced dyskinesias in the foreground. The very satisfactory clinical results, up to 12 months in the first case, confirm the observation of Laitinen, but with the difference that the approach discussed here is both nondestructive and reversible, and unwanted side effects are avoided.
Transduction of Nondividing Human Macrophages with Gammaretrovirus-Derived Vectors (2006)

It is commonly accepted that infection of nondividing cells by gammaretroviruses such as the murine leukemia viruses is inefficient due to their inability to cross the nuclear envelope barrier. Challenging this notion, we now show that human nondividing macrophages display a specific window of susceptibility to transduction with a Friend murine leukemia virus (F-MLV)-derived vector during their differentiation from monocytes. This finding suggests that factors other than the nuclear membrane govern permissiveness to gammaretroviral infection and raises the possibility of using the macrophage tropism of F-MLV in gene therapy.
Effects of the Estrous Cycle, Pregnancy, and Interferon Tau on 2′,5′-Oligoadenylate Synthetase Expression in the Ovine Uterus1 (2001)

The enzymes which comprise the 2',5'-oligoadenylate synthetase (OAS) family are interferon (IFN) stimulated genes which regulate ribonuclease L antiviral responses and may play additional roles in control of cellular growth and differentiation. This study characterized OAS expression in the endometrium of cyclic and pregnant ewes as well as determined effects of IFNtau and progesterone on OAS expression in cyclic or ovariectomized ewes and in endometrial epithelial and stromal cell lines. In cyclic ewes, low levels of OAS protein were detected in the endometrial stroma (S) and glandular epithelium (GE). In early pregnant ewes, OAS expression increased in the S and GE on Day 15. OAS expression in the lumenal epithelium (LE) was not detected in uteri from either cyclic or pregnant ewes. Intrauterine administration of IFNtau stimulated OAS expression in the S and GE, and this effect of IFNtau was dependent on progesterone. Ovine endometrial LE, GE, and S cell lines responded to IFNtau with induction of OAS proteins. In all three cell lines, the 40/46-kDa OAS forms were induced by IFNtau, whereas the 100-kDa OAS form appeared to be constitutively expressed and not affected by IFNtau. The 69/71-kDa OAS forms were induced by IFNtau in the S and GE cell lines, but not in the LE. Collectively, these results indicate that OAS expression in the endometrial S and GE of the early pregnant ovine uterus is directly regulated by IFNtau from conceptus and requires the presence of progesterone.
3D model-based segmentation of individual brain structures from magnetic resonance imaging data (1994)

This thesis addresses a specific problem of model-based segmentation; namely, the automatic identification and delineation of gross anatomical structures of the human brain based on their appearance in magnetic resonance images (MRI). The approach developed in this thesis depends on a general, iterative, hierarchical registration procedure and a 3-D digital model of human brain anatomy that contains both volumetric intensity-based data and geometric atlas data that co-exist in a brain-based stereotaxic coordinate system. The model contains features derived from an MRI atlas of gross neuroanatomy, that is the result of an intensity average of 305 brains created with an automatic stereotaxic registration procedure developed here.
p300/CREB-Binding Protein Enhances the Prolactin-Mediated Transcriptional Induction through Direct Interaction with the Transactivation Domain of Stat5, but Does Not Participate in the Stat5-Mediated Suppression of the Glucocorticoid Response (1998)

Stat5 was discovered as a PRL-induced member of the Stat (signal transducer and activator of transcription) family. Its induction by many other cytokines and interleukins suggests that Stat5 plays a crucial role not only in mammary epithelial, but also in hematopoietic cells. Cell type- and promoter-specific functions of Stat5 are most likely modulated by the interaction with other transcription factors. We recently showed cross-talk between Stat5 and the glucocorticoid receptor. The activated glucocorticoid receptor forms a complex with Stat5 and enhances Stat5-mediated transcriptional induction. Conversely, Stat5 diminishes the induction of glucocorticoid-responsive genes. Here, we investigated the role of p300/CBP(CREB-binding protein), a transcriptional coactivator of several groups of transcription factors, in Stat5-mediated transactivation and in the cross-talk between Stat5 and the glucocorticoid receptor. p300/ CBP acts as a coactivator of Stat5. Its ectopic expression enhances PRL-induced Stat5-mediated transcriptional activation. Consistent with this observation, we find that the adenovirus E1A protein, which binds to p300/CBP, suppresses Stat5-induced transcriptional activation. This inhibition requires the Stat5 transactivation domain and the p300/CBP binding site of E1A. Coimmunoprecipitation and mammalian two-hybrid assays demonstrate a direct interaction between the carboxyl-terminal transactivation domain of Stat5 and p300/CBP. p300/CBP also positively interacts with the glucocorticoid receptor and enhances glucocorticoid receptor-dependent transcriptional activation of the mouse mammary tumor virus-long terminal repeat promoter. Overexpression of p300/CBP does not counteract the Stat5-mediated inhibition of glucocorticoid receptor-dependent transactivation, i.e. the repression of the glucocorticoid response by Stat5 is not a consequence of competition for limiting amounts of p300/CBP.
Identification of γ-Endorphin-Generating Enzyme as Insulin-Degrading Enzyme (1996)

The EL-4 thymoma cell line contains a peptidase which converts beta-endorphin to beta-endorphin 1-17 (gamma-endorphin), beta-endorphin 1-18, and their corresponding C-terminal fragments. This enzyme was purified approximately 700-fold to a single band on an SDS-polyacrylamide gel (106 kDa) in 16% yield. Estimation of the native molecular weight by molecular sieve chromatography gave a value of approximately 220 kDa, indicating that this enzyme is a dimer. Peptide sequencing demonstrated this activity can be attributed to insulin degrading enzyme, a previously described member of the inverzincin family (Hooper, 1994). Kinetic studies with a number of peptide substrates indicate that the enzyme preferentially cleaves on the amino side of hydrophobic or basic residues. However, the substrate specificity is more complex since not all basic and hydrophobic residues in a peptide are cleaved. The enzyme exhibits a requirement for a P'2 residue. On the basis of kcat/K(m) values, insulin, growth hormone releasing factor, and beta-endorphin are nearly equivalent substrates for the enzyme; however, growth hormone releasing factor and beta-endorphin exhibit a 40-fold higher kcat, but a 10-fold decreased affinity relative to insulin. A role for insulin-degrading enzyme as both a beta-endorphin-processing and -inactivating enzyme is implicated from these studies.
Angiogenesis: a new prognostic marker for breast cancer. (1998)

The past two decades have seen a huge increase in the study of angiogenesis or neovascularisation. With it has come realisation of the crucial role that angiogenesis plays in the growth and metastatic potential of carcinoma. One area where there has been particular interest is in the measurement of angiogenesis in breast carcinoma, with the hope that it may prove to be an independent prognostic indicator. As advances in breast cancer treatment have increased, so has the importance of assigning a patient to the optimal treatment regime. A number of studies have assessed tumour vascularity on sections immunostained with endothelial markers. Of these, the majority have found that highly vascular carcinomas have a poorer prognosis than those with low vascularity, although several studies have reported no correlation. These discrepancies may, in large part, be due to variations in the methods employed by different laboratories. At present, microvessel quantification in breast carcinoma has yet to prove it can reliably add a measurable amount of information to that already available from established prognostic indicators such as tumour size, nodal status, histological grade, and hormone receptor status. Although promising, further study is needed to establish the true value of measuring angiogenesis in breast cancer.
PP.16.16 (2015)

Objective: Cardiovascular mortality is higher in women than in men, and this difference is still increasing. The awareness about hypertension, the main risk factor of cardiovascular disease, is high. Nevertheless, still a large part of the women is not adequately controlled. It has not been established, whether uterine fibroids might be a new female-specific risk factor for hypertension. Therefore, we systematically reviewed the association between uterine fibroids and hypertension. Design and method: We systematically searched MEDLINE, EMBASE, CENTRAL, LILACS, and Google Scholar to identify studies on uterine fibroids and hypertension. The search was conducted with no restriction to language or publication date. Two reviewers independently extracted the data. The primary outcome was the prevalence of hypertension in women with and without uterine fibroids. Results: We retrieved 1,807 papers. Eleven observational studies met our inclusion criteria, which included a total of 2,219 women with and 9,995 women without fibroids. Ten of the eleven studies showed a positive association between fibroids and hypertension, resulting in a pooled odds ratio of 1.88 (95% CI: 1.44 to 2.46, p <0.001, Figure 1). Subgroup analyses revealed that the association between hypertension and fibroids was significant in women with observed hypertension as well as self-reported hypertension, with odds ratios of 1.90 (95% CI: 1.34 to 2.69) and 1.83 (95% CI: 1.16 to 2.89) respectively. Furthermore, both histopathologically confirmed fibroids and self-reported fibroids were associated with hypertension; odds ratios 1.90 (95% CI: 1.34 to 2.69) and 1.91 (95% CI: 1.18 to 3.10) respectively.Conclusions: Uterine fibroids are associated with hypertension. More awareness about this association might reduce the gender gap in cardiovascular disease. Furthermore, more research is needed to explore the nature of this association.
For Protection from HIV-1 Infection, More Might Not Be Better: a Systematic Analysis of HIV Gag Epitopes of Two Alleles Associated with Different Outcomes of HIV-1 Infection (2011)

A subset of women in the Pumwani Sex Worker Cohort, established in 1985 in Nairobi, Kenya, remains uninfected despite repeated high-risk exposure (HIV-exposed, seronegative [HESN]) through active sex work. This HESN phenotype is associated with several alleles of human leukocyte antigens (HLAs) and specific CD8(+) and CD4(+) T cell responses to HIV-1. The associations of HLA alleles with differential HIV-1 infection are most likely due to their different abilities to present antigen and the different immune responses they induce. The characteristics of epitopes of HLA alleles associated with different outcomes of HIV-1 infection might therefore point to a vital clue for developing an effective vaccine. In this study, we systematically analyzed HIV-1 clade A and D Gag CD8(+) T cell epitopes of two HLA class I alleles associated with different outcomes of HIV-1 infection. Binding affinity and off-rates of the identified epitopes were determined. Gamma interferon (IFN-γ) enzyme-linked immunospot (ELISpot) assays with patient peripheral blood mononuclear cells (PBMCs) validated the epitopes. Epitope-specific CD8(+) T cells were further phenotyped for memory markers with tetramer staining. Our study showed that the protective allele A*01:01 recognizes only three Gag epitopes. By contrast, B*07:02, the allele associated with susceptibility, binds 30 epitope variants. These two alleles differ most importantly in the spectrum of Gag epitopes they can present and not in affinity, off-rates, the location of the epitopes, or epitope-specific Tem/Tcm frequencies. The binding of more epitopes and strong IFN-gamma ELISpot responses are associated with susceptibility to HIV-1 infection, while more focused antigen recognition of multiple subtypes is protective. Rational vaccine design should take these observations into account.
Treatment and Survival Outcome for Molecular Breast Cancer Subtypes in Black Women (2008)

The basal cell-like subtype is associated with a worse prognosis, a higher incidence of distant metastasis, and may be more prone to local recurrence when managed with breast-conserving therapy.
High speed and low complexity XOR-free technique based data encoder architecture (2017)

Designers are increasingly trust on field programmable gate array (FPGA) based emulation to evaluate the performance of Convolutional Encoder. Optimization of XOR operators is the very important concern while implementing polynomials over Galosis Field(2). The proposed approach requires low power high efficient parity check code decoder architecture for error detection and correction applications. This method completely removes XOR processing operation, thereby the cost is reduced. These codes have been adopted in latest wireless standards such as satellite and mobile communications, So they posses superior error detecting and correcting capabilities. However this novel schemes are implement to use Read-Only Memory (ROM) with a preprocessed addressing operation to reduce ROM size by nearly 75%. Experiment result of the new architecture reduces the dynamic power upto 27.3% and HW cost upto 20%. This architecture have atleast tens of times lower complexity as compared to conventional method. As per the technology scales, memory devices becomes larger, more powerful and low power consumption based error correcting codes are needed.
Chemical mechanisms in bioluminescence (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical mechanisms in bioluminescenceFrank McCapraCite this: Acc. Chem. Res. 1976, 9, 6, 201–208Publication Date (Print):June 1, 1976Publication History Published online1 May 2002Published inissue 1 June 1976https://pubs.acs.org/doi/10.1021/ar50102a001https://doi.org/10.1021/ar50102a001research-articleACS PublicationsRequest reuse permissionsArticle Views2201Altmetric-Citations148LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Antibody-based biosensor for breast cancer with ultrasonic regeneration (2000)

We describe a novel method and instrumental setup for regenerating antibodies immobilized on a fiberoptic probe of an immunosensor using ultrasonic irradiation with broadband imaging transducers. The instrumental setup and irradiation conditions for antibody regeneration using ultrasound are described. The results of the measurements with antibody against breast cancer antigen illustrate the effectiveness and potential of the regenerable immunosensor. A 65% removal of the antigens bound to the Mab immobilized on the fiber surface is attained after ultrasound regeneration.
Are Natural Deep Eutectic Solvents the Missing Link in Understanding Cellular Metabolism and Physiology? (2011)

Over the past decade, metabolomics has developed into a major tool for studying the metabolism of organisms and cells, and through this approach much has been learned about metabolic networks and the reactions of organisms to various external conditions ([Lay et al., 2006][1]). Most of this work
Recommendation for block cipher modes of operation : (2010)

The Information Technology Laboratory (ITL) at the National Institute of Standards and Technology (NIST) promotes the U.S. economy and public welfare by providing technical leadership for the Nation's measurement and standards infrastructure. ITL develops tests, test methods, reference data, proof of concept implementations, and technical analyses to advance the development and productive use of information technology. ITL's responsibilities include the development of technical, physical, administrative, and management standards and guidelines for the cost-effective security and privacy of sensitive unclassified
Treatment of Essential Telangiectasias with an Intense Pulsed Light Source (PhotoDerm VL) (1997)

The PhotoDerm VL is an innovative, highly effective, and comparably safe therapeutic alternative to the laser in the treatment of ETE. The rate of cosmetically relevant side effects is considerably smaller, the patient compliance is excellent, and the method can be applied easily in an outpatient setting.