gebhart/patrepeval huggingface dataset:
Gene structure of mammalian acetylcholinesterase. Alternative exons dictate tissue-specific expression. (1991)

The genes encoding mouse and human acetylcholinesterases have been cloned from genomic and cosmid libraries. Restriction analysis and a comparison of sequence with the cDNAs have defined the exon-intron boundaries. In mammals, three invariant exons encode the signal peptide and the amino-terminal 535 amino acids common to all forms of the enzyme whereas alternative exon usage of the next exon accounts for the structural divergence in the carboxyl termini of the catalytic subunits. mRNA protection studies show that the cDNA encoding the hydrophilic catalytic subunits represents the dominant mRNA species in mammalian brain and muscle whereas divergent mRNA species are evident in cells of hematopoietic origin (bone marrow cells and a erythroleukemia cell line). Analyses of mRNA species in these cells and the genomic sequence have enabled us to define two alternative exons in addition to the one found in the cDNAs; they encode unique carboxyl-terminal sequences. One mRNA consists of a direct extension through the intervening sequence between the common exon and the 3' exon deduced from the cDNA. This sequence encodes a subunit lacking the cysteine critical to oligomer formation. Another mRNA results from a splice that encodes a stretch of hydrophobic amino acids immediately upstream of a stop codon. This exon, when spliced to the upstream invariant exons, should encode glycophospholipid-linked species of the enzyme. Homologous sequence, identity of exon-intron junctions, and identity of position of the stop codon are seen for this region in mouse and human. Polymerase chain reactions carried out across the expected intron region and mRNA protection studies show that this splice occurs in mouse bone marrow and erythroleukemia cells yielding the appropriate cDNA.
Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagen-derived peptides. (1978)

The chemotactice response of human dermal fibroblasts of type I, II, and III human collagens and collagen-derived peptides was quantitated by an in vitro assay. All three native human collagens and constituent alpha chains can serve as chemoattractants for fibroblasts in vitro. When type I, II, and III collagens were digested by bacterial collagenase, the resulting peptides were also chemotactic. In addition, synthetic di- and tripeptides containing hydroxyproline were also chemotactic for fibroblasts. Since collagen is degraded and remodeled at sites of tissue injury and inflammation, these findings suggest that collagen and collagen-degradation peptides might function as chemotactic stimuli for fibroblasts in vivo and attract these cells to effect repair of damaged tissue.
Natural linewidth chemical shift imaging (NL‐CSI) (2006)

The discrete Fourier transform (FT) is a conventional method for spatial reconstruction of chemical shifting imaging (CSI) data. Due to point spread function (PSF) effects, FT reconstruction leads to intervoxel signal leakage (Gibbs ringing). Spectral localization by imaging (SLIM) reconstruction was previously proposed to overcome this intervoxel signal contamination. However, the existence of magnetic field inhomogeneities creates an additional source of intervoxel signal leakage. It is demonstrated herein that even small field inhomogeneities substantially amplify intervoxel signal leakage in both FT and SLIM reconstruction approaches. A new CSI data acquisition strategy and reconstruction algorithm (natural linewidth (NL) CSI) is presented that eliminates effects of magnetic field inhomogeneity-induced intervoxel signal leakage and intravoxel phase dispersion on acquired data. The approach is based on acquired CSI data, high-resolution images, and magnetic field maps. The data are reconstructed based on the imaged object structure (as in the SLIM approach) and a reconstruction matrix that takes into account the inhomogeneous field distribution inside anatomically homogeneous compartments. Phantom and in vivo results show that the new method allows field inhomogeneity effects from the acquired MR signal to be removed so that the signal decay is determined only by the "natural" R2 relaxation rate constant (hence the term "natural linewidth" CSI).
A systematic review of lactoferrin use in dermatology (2016)

Lactoferrin is a glycoprotein widely present in mammalian secretions and possesses documented protective effects, including antimicrobial and anti-inflammatory properties. While its therapeutic use is being investigated for a myriad of diseases, there is increasing interest in its application for skin disease. Our objective was to systematically review the clinical evidence for the use and efficacy of lactoferrin for the treatment of dermatological conditions. Pubmed and Embase databases were searched for clinical studies evaluating lactoferrin for dermatological conditions. A total of six studies were reviewed. Of the current clinical trials, there is encouraging evidence to suggest that lactoferrin may be beneficial in acne, psoriasis, and diabetic ulcerations. Although the current evidence is promising, further research is necessary to establish lactoferrin as complementary therapy in the clinical setting.
Hydrogel Retaining Toxin‐Absorbing Nanosponges for Local Treatment of Methicillin‐Resistant Staphylococcus aureus Infection (2015)

A hybrid nanomaterial integrating unique toxin-absorbing nanosponges with hydrogel is developed for local antivirulence therapy against methicillin-resistant Staphylococcus aureus (MRSA) infection. The hydrogel composition is optimized to retain toxin nanosponges after administration while not compromising toxin transport into the gel for neutralization. Mice treated with the nanosponge–hydrogel hybrid show markedly reduced MRSA skin lesion development.
Distributed Time Division Multiple Access (DTDMA) - Principles and Analysis Techniques (1983)

The principles of Distributed Time Division Multiple Access (DTDMA) technology are delineated. DTDMA is currently being implemented as the U.S. Navy's Joint Tactical Information Distribution System (JTIDS) in a full scale development program. This effort is under contract to TADCOM, a joint venture of ITT Avionics Division and Hughes Aircraft Company. JTIDS is a spread spectrum system which supports concurrent multi-CNI function (Communication, Navigation and Identification), multi-terminal and multi-net operation. Efficiency, flexibility and high jamming resistance is obtained by utilizing in its design the three ambiguities of signal space: time, frequency and code; and also sharing terminal resources among all of the functions (integrated CNI system). A combined simulation/analytic technique is described which provides design information and predicts terminal capacity and anti-jam performance trade-offs, for both digital data and digital voice data links.
Transforming growth factor-alpha-Pseudomonas exotoxin fusion protein (TGF-alpha-PE38) treatment of subcutaneous and intracranial human glioma and medulloblastoma xenografts in athymic mice. (1994)

Epidermal growth factor receptor (EGFR) is amplified or overexpressed in many malignant gliomas and other primary brain tumors but is low or undetectable in normal brain. In the present study, this differential expression has been exploited for targeted brain tumor therapy using a TGF-alpha-Pseudomonas exotoxin recombinant toxin, TGF-alpha-PE38. In vitro experiments demonstrate that the cytotoxicity of this fusion protein is primarily determined by tumor EGFR expression and that TGF-alpha-PE38 cytotoxicity is abolished by pretreatment with excess epidermal growth factor. Treatment with i.p. TGF-alpha-PE38 in nude mice bearing glioblastoma or medulloblastoma s.c. xenografts produced tumor regression and growth delay. For intracranial xenograft implants treated with i.p. TGF-alpha-PE38, significant increases in median survival were noted only for tumors with the highest EGFR expression. However, intracranial tumors treated with a single intratumoral injection of TGF-alpha-PE38 showed increased survival in all xenografts tested. These results indicate that TGF-alpha-PE38 is active against primary human brain tumors ranging from moderate to high EGFR expression. For intracranial tumors, however, the higher survival rates produced by intracranial injection of TGF-alpha-PE38 than by continuous i.p. administration suggest that increased drug clearance or impaired drug delivery reduces the efficacy of systemic TGF-alpha-PE38. Direct delivery of TGF-alpha-PE38 into brain tumors by controlled-release biodegradable polymers or intratumoral implanted catheters, or intrathecal administration into the colony stimulating factor of patients with leptomeningeal metastasis, may represent clinically useful applications of recombinant toxin therapy in tumors with high EGFR expression.
A Novel Spliced Form of SH2-Containing Inositol Phosphatase Is Expressed During Myeloid Development (1999)

SH2-containing Inositol Phosphatase (SHIP) is a 145 kD protein expressed in hematopoietic cells. SHIP is phosphorylated on tyrosine after receptor binding by several cytokines and has a negative role in hematopoiesis. We cloned a murine complementary DNA (cDNA) sequence for an isoform of SHIP with an internal 183 nucleotide deletion, encoding a protein 61 amino acids shorter than 145 kD SHIP. This deletion eliminates potential SH3-domain binding regions and a potential binding site for the p85 subunit of Phosphatidylinositol 3-Kinase. Using polyclonal anti-SHIP antibodies, we and others have previously observed a 135 kD SHIP isoform that is coexpressed with 145 kD SHIP. Here, we used monoclonal antibodies raised against the region deleted in the spliced form to show that the product of the novel spliced SHIP cDNA is antigenically identical to the 135 kD SHIP isoform. Like 145 kD SHIP, 135 kD SHIP expression was induced on differentiation of bone marrow cells. After macrophage colony-stimulating factor (M-CSF) stimulation of FDC-P1(Fms) myeloid cells, both 145 and 135 kD SHIP forms were tyrosine phosphorylated and could be coimmunoprecipitated with antibodies to Shc and Grb2. However, experiments showed only a weak association of 135 kD SHIP with p85. A potentially analogous 135 kD SHIP species also appears in human differentiated leukocytes.
On the Locality of Codeword Symbols (2012)

Consider a linear [ <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">n</i> , <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</i> , <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">d</i> ] <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">q</sub> code <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">C</i> . We say that the <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">i</i> th coordinate of <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">C</i> has locality <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</i> , if the value at this coordinate can be recovered from accessing some other <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</i> coordinates of <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">C</i> . Data storage applications require codes with small redundancy, low locality for information coordinates, large distance, and low locality for parity coordinates. In this paper, we carry out an in-depth study of the relations between these parameters. We establish a tight bound for the redundancy <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">n</i> - <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</i> in terms of the message length, the distance, and the locality of information coordinates. We refer to codes attaining the bound as optimal. We prove some structure theorems about optimal codes, which are particularly strong for small distances. This gives a fairly complete picture of the tradeoffs between codewords length, worst case distance, and locality of information symbols. We then consider the locality of parity check symbols and erasure correction beyond worst case distance for optimal codes. Using our structure theorem, we obtain a tight bound for the locality of parity symbols possible in such codes for a broad class of parameter settings. We prove that there is a tradeoff between having good locality and the ability to correct erasures beyond the minimum distance.
Transfection of Mammalian Cells via Lipofection (2003)

The use of baker’s yeast, Saccharomyces cerevisiae, for the cloning of extremely large genomic intervals (exceeding 1 Mb) was made possible with the development of yeast artificial chromosomes (YACs) (). YACs are linear molecules containing all the control elements necessary for stable replication and segregation during the yeast life cycle. This cloning strategy was used to develop a technology for shuttling large genomic intervals back and forth between mammals and yeast.
Clinical Science Cusp Reinforcement by the Acid-etch Technique (1984)

Strain gauges were mounted on 12 maxillary pre-molars which were subjected to a sequence of restorative procedures for MOD preparations. An occlusal stress was applied using servohydraulics, and the cuspal flexure was assessed using a strain gauge. Two bonded and three non-bonded restorative procedures were tested for each tooth. The two bonded conditions showed significantly higher cuspal reinforcement when compared with the MOD preparation and the non-bonded restorative procedures. The non-bonded restorations showed some intragroup differences, with one non-bonded composite showing useful cuspal reinforcement. However, this was much less than that afforded by the bonded technique. The deformation of the cusp under occlusal force in restorations bonded by the acid-etch technique showed much less hysteresis when compared with non-bonded restorations.
Loss of endothelial pertussis toxin-sensitive G protein function in atherosclerotic porcine coronary arteries. (1991)

Pertussis toxin, an irreversible inhibitor of some G proteins, inhibits endothelium-dependent relaxations to certain agonists in porcine coronary arteries. In the present study, the effects of the toxin were examined on endothelium-dependent and -independent relaxations of hypercholesterolemic and atherosclerotic porcine coronary arteries to assess the functional state of the endothelial pertussis toxin-sensitive G protein. Male Yorkshire pigs were maintained on either a regular diet (control group, n = 7) or a 2% high-cholesterol diet (cholesterol-fed group, n = 7) for 10 weeks. After the initial 2 weeks of maintenance, animals in both groups underwent balloon catheter removal of the endothelium of the left anterior descending or left circumflex coronary arteries. Endothelium-dependent responses were examined in vitro after 10 weeks of maintenance; at this time, a full lining of endothelial cells in both left coronary arteries was confirmed histologically. In arteries with endothelium of the control group (normal responses), pertussis toxin significantly inhibited the endothelium-dependent relaxations to serotonin, UK14304 (a selective alpha 2-adrenergic receptor agonist), and thrombin but not those to ADP, bradykinin, or the calcium ionophore A23187. In previously denuded arteries of the control group (effects of endothelial regeneration alone) or intact arteries of the cholesterol-fed group (effects of hypercholesterolemia alone), the relaxations to serotonin, UK14304, and thrombin were impaired significantly; those relaxations were impaired further in previously denuded arteries of the cholesterol-fed group (effects of atherosclerosis). The inhibitory effects of pertussis toxin were significantly reduced after endothelial regeneration and in hypercholesterolemia and were almost absent in atherosclerosis.(ABSTRACT TRUNCATED AT 250 WORDS)
JS-K has potent anti-angiogenic activityin vitroand inhibits tumour angiogenesis in a multiple myeloma modelin vivo (2010)

JS-K is a potent inhibitor of angiogenesis in vitro and tumour vessel growth in vivo. As such, it establishes a new class of antineoplastic agent that targets the malignant cells directly as well as their microenvironment.
Combined local and holistic facial features for age-determination (2010)

This paper presents an advanced age-determination technique that combines holistic and local features derived from an image of the face. A 30×1 Active Appearance Model (AAM) linear encoding of each face is produced to work as holistic features. Meanwhile, local features are extracted by using Local Ternary Patterns (LTP). These combined features are used to classify faces into one of two age groups (age-classification). An age-determination function is then constructed for each age group in accordance with physiological growth periods for humans - pre-adult (youth) and adult. Compared to published results, this method yields the highest accuracy rates in overall mean absolute error (MAE), mean absolute error per decade of life (MAE/D), and cumulative match score.
Cyclin D1 overexpression promotes cardiomyocyte DNA synthesis and multinucleation in transgenic mice. (1997)

D-type cyclin/cyclin-dependent kinase (CDK) complexes regulate transit through the restriction point of the cell cycle, and thus are required for the initiation of DNA synthesis. Transgenic mice which overexpress cyclin D1 in the heart were produced to determine if D-type cyclin deregulation would alter myocardial development. Cyclin D1 overexpression resulted in a concomitant increase in CDK4 levels in the adult myocardium, as well as modest increases in proliferating cell nuclear antigen and CDK2 levels. Flow cytometric and morphologic analyses of dispersed cell preparations indicated that the adult transgenic cardiomyocytes had abnormal patterns of multinucleation. Histochemical analyses confirmed a marked increase in number of cardiomyocyte nuclei in sections prepared from the transgenic mice as compared with those from control animals. Tritiated thymidine incorporation analyses revealed sustained cardiomyocyte DNA synthesis in adult transgenic hearts.
The vaccine handbook :a practical guide for clinicians (2004)

The vaccine handbook :a practical guide for clinicians , The vaccine handbook :a practical guide for clinicians , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Progestogen administration in pregnancy may prevent preterm delivery (1990)

Two recently published meta-analyses of controlled trials of a wide variety of progestational agents, used in pregnancy (Daya 1989; Goldstein et al. 1989), prompted this third meta-analysis of placebo-controlled trials involving the prophylactic use of a single agent, 17 alpha-hydroxyprogesterone caproate. Of seven relevant published reports of controlled trials, six had involved women considered to be a high risk of miscarriage or preterm birth. This analysis provides no support for the view that 17 alpha-hydroxyprogesterone caproate protects against miscarriage, but suggests that it does reduce the occurrence of preterm birth. The latter effect was reflected in a reduced rate of low birthweight babies, but not in a statistically significant reduction in perinatal mortality and morbidity. The difference between this meta-analysis and the two earlier meta-analyses illustrates the problems both of selective sub-grouping and of comprehensive pooling of data from small trials.
Texasweed (Caperonia Palustris) Control in Soybean with Postemergence Herbicides (2007)

Field and greenhouse studies were conducted in 2000 and 2001 in Mississippi to determine the most effective POST herbicide programs for control of Texasweed in glyphosate-resistant soybean. In the field, Texasweed plants recovered from most POST herbicide treatments, and plant death rarely occurred. A sequential application of 1,100 g ae/ha glyphosate followed by 840 g/ha glyphosate was the only treatment that controlled at least 90% of Texasweed 4 wk after treatment. Texasweed control ranged from 80 to 87% for lower rates of glyphosate applied once or twice and 390 g ai/ha fomesafen. The addition of fomesafen or other herbicides to glyphosate did not improve control compared with glyphosate alone. In the greenhouse, glyphosate at 560 g/ha controlled 93 and 90% of one- and two-leaf plants, respectively, but at least 1,400 g/ha was required to obtain 90% control of four-leaf plants. Fomesafen at 260 g/ha controlled 93 to 99% of one- to four-leaf Texasweed plants in the greenhouse.Nomenclature: Fomesafen, glyphosate, Texasweed, Caperonia palustris (L.) St. Hil, soybean, Glycine max (L.) Merr
Autologous Expanded Adipose-Derived Stem Cells for the Treatment of Complex Cryptoglandular Perianal Fistulas (2012)

Background: Autologous adipose-derived stem cells may represent a novel approach for the management of complex fistula-in-ano. After successful phase I and II clinical trials, a phase III trial was performed to investigate the safety and efficacy. Design: In this multicenter, randomized, single-blind, add-on clinical trial, 200 adult patients from 19 centers were randomly assigned to receive 20 million stem cells (group A, 64 patients), 20 million adipose-derived stem cells plus fibrin glue (group B, 60 patients), or fibrin glue (group C, 59 patients) after closure of the internal opening. Fistula healing was defined as reepithelization of the external opening and absence of collection >2 cm by MRI. If the fistula had not healed at 12 weeks, a second dose (40 million stem cells in groups A and B) was administered. Patients were evaluated at 24 to 26 weeks (primary end point) and at 1 year (long-term follow-up). Results: All results are according to the “blinded evaluator” assessment. After 24 to 26 weeks, the healing rate was 39.1%, 43.3%, 37.3% in groups A, B, and C (p = 0.79). At 1 year, the healing rates were 57.1%, 52.4%, and 37.3 % (p = 0.13). On analysis of the subpopulation treated at the technique’s pioneer center, healing rates were 54.55%, 83.33%, and 18.18%, at 24 to 26 weeks (p < 0.001). No SAEs were reported. Conclusions: In treatment of complex fistula-in-ano, a dose of 20 or 60 million adipose-derived stem cells alone or in combination with fibrin glue was considered a safe treatment, achieving healing rates of approximately 40% at 6 months and of more than 50% at 1-year follow-up. It was equivalent to fibrin glue alone. No statistically significant differences were found when the 3 groups where compared. Clinical trials registration: www.clinicaltrials.gov, identifier NCT00475410; Sponsor, Cellerix SA.