gebhart/patrepeval huggingface dataset:
Renal intracortical blood flow and renin secretion after denervation by 6-hydroxydopamine (1982)

A new method of unilateral renal denervation (DNX) was developed in dogs by rapidly injecting 6-hydroxydopamine (6-OHDA) into the renal artery with simultaneous collection of the venous effluent to avoid systemic effects. The procedure induced a 93.1 +/- 2.7% destruction of the adrenergic innervation as assessed by a histochemical fluorescence technique. A comparative study of both kidneys was carried out 3 days after DNX. Renal blood flow (RBF) and its cortical distribution were measured with radioactive microspheres at mean perfusion pressures of 117 mmHg (1 mmHg = 133.322 Pa) (normotension) and 70 mmHg (hypotension). The comparison of renal perfusion between kidney halves revealed a remarkable perfusion symmetry during normotension and hypotension in both the intact and the DNX kidney. Renal DNX by 6-OHDA did not alter RBF when the kidneys were perfused at normotension. During hypotension, RBF fell by 45% on the control side, while a nonsignificant decrease was noted in the DNX kidney. The redistribution of blood flow to inner cortical zones was less marked in the DNX side during hypotension. The DNX kidney did not release renin during normotension, but exhibited a normal renin response to hypotension. These data are partly in agreement with classical denervation models, and indicate that (1) denervation by 6-OHDA protects renal perfusion during hypotension; and (2) the redistribution of blood flow to inner cortical nephrons is independent of release of renin, but modulated by adrenergic innervation.
Growth Hormone and the Heart (1994)

GH exerts direct effects on myocardial growth and function. Evidence from laboratory models shows that GH (or IGF-I) induces mRNA expression for specific contractile proteins and myocyte hypertrophy. Furthermore, GH increases the force of contraction and determines myosin phenoconversion toward the low ATPase activity V3 isoform. These data provide plausible explanations for the cardiac abnormalities observed in clinical settings of excessive or defective GH production. In acromegaly, the functional consequences of GH excess initially prevail (hyperkinetic syndrome), followed by alterations of cardiac function when myocardial hypertrophy develops. This involves both ventricles and is purposeless because it occurs without increased wall stress. Hypertrophy also entails proliferation of the myocardial fibrous tissue that leads to interstitial remodeling. The functional consequence is an impaired ventricular relaxation that causes a diastolic dysfunction, followed by impairment of systolic function. In untreated disease, cardiac performance slowly but inexorably deteriorates and heart failure eventually develops. Several lines of evidence support the specificity of heart disease in acromegaly. Particularly demonstrative are the recent studies in which GH production was suppressed by octreotide, with a consequent significant regression of hypertrophy and improvement of cardiac dysfunction. It is not yet established whether full recovery of normal cardiac morphology and function is possible after correction of GH excess. The point is not a minor one since the possibility to revert, albeit partially, myocardial fibrosis is of great relevance to the control of cardiac hypertrophy in general. GHD leads to a reduced mass of both ventricles and to impaired cardiac performance with low heart rate (hypokinetic syndrome). These alterations are particularly evident during physical exercise and might provide an important contribution to the reduced exercise capacity of GHD patients, in addition to the reduced muscle mass and strength. The data also support a role of GH in the maintenance of a normal cardiac structure and performance. The hypokinetic syndrome is well documented in young patients in whom GHD began very early in their childhood. In contrast, the data in adult-onset GHD are less consistent. This suggests that the consequences of GHD are more relevant if the disorder starts during early heart development. As observed with other abnormalities associated with GHD, cardiac dysfunction is also susceptible to marked improvement by hrGH. This observation lends further support to the proposal to treat these patients with replacement therapy.
Luminescence and NMR studies of the conformational isomers of lanthanide complexes with an optically active polyaza polycarboxylic macrocycle (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLuminescence and NMR studies of the conformational isomers of lanthanide complexes with an optically active polyaza polycarboxylic macrocycleHarry G. Brittain and Jean F. DesreuxCite this: Inorg. Chem. 1984, 23, 26, 4459–4466Publication Date (Print):December 1, 1984Publication History Published online1 May 2002Published inissue 1 December 1984https://pubs.acs.org/doi/10.1021/ic00194a012https://doi.org/10.1021/ic00194a012research-articleACS PublicationsRequest reuse permissionsArticle Views435Altmetric-Citations82LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Experimental study of a real-time calibration procedure of a CDMA/TDD multiple antenna terminal (2003)

We have presented in this paper some experimental real-time calibration results of a CDMA transmitter/receiver for TDD systems by calculating the RF devices' impulse responses with a least square algorithm. Radiation pattern measurements have allowed to verify the effectiveness of this calibration method.
Immunohistochemical analysis of tumor antigen saturation following injection of monoclonal antibody G250. (1999)

Clinical tumor targeting studies with monoclonal antibody (mAb) G250 showed excellent targeting of primary renal cell carcinomas (RCC). However, tumor uptake decreased at higher mAb G250 doses, suggesting saturation of the G250 antigenic determinants. In this immunohistochemical study we investigated the saturability of G250 antigen sites after i.v. administration of mAb G250 at various protein doses in nude mice with RCC xenografts. Five groups of mice received five different protein doses (1, 3, 10, 30 or 100 micrograms) of murine mAb G250. Three days post injection mice were killed and the tumors were removed. Free G250 antigen sites, i.e., not targeted by the i.v. injected murine mAb G250 were determined by immunohistochemical staining with chimeric mAb G250. Distinct staining of the G250 antigen was observed only at the 1 and 3 micrograms dose whereas G250 antigen staining at higher doses was virtually negative. The results of this study indicate that saturation of antigen occurs at relatively low doses of i.v. administered mAb G250. Apparently, all antigenic determinants present on the RCC tumor cells were targeted, while previous preclinical studies suggested that i.v. administration of mAb G250 only saturated the accessible antigen sites.
Synthetic Zinc Finger Nuclease Design and Rapid Assembly (2011)

Engineered zinc finger nucleases (ZFNs) are a tool for genome manipulation that are of great interest to scientists in many fields. To meet the needs of researchers wishing to employ ZFNs, an inexpensive, rapid assembly procedure would be beneficial to laboratories that do not have access to the proprietary reagents often required for ZFN production. Using freely available sequence data derived from the Zinc Finger Targeter database, we developed a protocol for synthesis and directed insertion of user-defined ZFNs into a versatile plasmid expression system. This oligonucleotide-based isothermal DNA assembly protocol was used to determine whether we could generate functional nucleases capable of endogenous gene editing. We targeted the human α-l-iduronidase (IDUA) gene on chromosome 4, mutations of which result in the severe lysosomal storage disease mucopolysaccharidosis type I. In approximately 1 week we were able to design, assemble, and test six IDUA-specific ZFNs. In a single-stranded annealing assay five of the six candidates we tested performed at a level comparable to or surpassing previously reported ZFNs. One of the five subsequently showed nuclease activity at the endogenous genomic IDUA locus. To our knowledge, this is the first demonstration of in silico-designed, oligonucleotide-assembled, synthetic ZFNs, requiring no specialized templates or reagents that are capable of endogenous human gene target site activity. This method, termed CoDA-syn (context-dependent assembly-synthetic), should facilitate a more widespread use of ZFNs in the research community.
An Extensible and Retargetable Code Generation Framework for Actor Models (2009)

Model-based code generation (MBCG) is a new field of research that arises from the advancement of high-level modeling languages. It is the syntactical transformation of a source model into some textual representation or program. Its applicability ranges from accelerating execution, targeting various platforms to enhancing model interoperability. MBCG is a key agent in sharing models across different research communities and maximizing the impact of the model-based design methodology. However, because of the raised level of abstractions, code generation in a model-based environment brings up a new set of problems beyond traditional compiler research. &lt;p&gt; Using Ptolemy II as an exemplar modeling environment, this report explores the challenges and problems that exist for model-based code generation. The report describes a reusable code generation framework built using two simple design patterns, code template and adapter, that are well-suited for an actor-based environment. The extensible infrastructure helps simplify the process for future experiments, especially for new models of computation (MoCs). The report tackles the challenges posed by configurable higher-order actors. It also demonstrates a system for incorporating multiple datatypes and specializing polymorphic functions to increase code efficiency.
Soundings: No more bed blocking (2002)

Here in the Greater Auchendreich Health Board area—where recent reorganisation has brought together primary care and acute services, along with strong and welcome board-level input from our colleagues in the world of social work—bed-blocking is no more.

Instead it is called delayed discharge, but despite the welcome advance in nomenclature the statistics remain depressingly familiar. Already cynics are muttering that our latest reorganisation can do little to address one of the central issues faced by all major healthcare providers in Auchendreich today.

Such pessimism was confounded at a major workshop, “Tackling Delayed Discharge: Towards a Whole-Auchendreich Approach,” held last Friday in the Auchendreich Hilton (formerly the Dreichmuir Station Hotel). It featured strong inter-agency, multidisciplinary, and public/private participation, and what made the day so special was something quite new to us here in Auchendreich: high-level event-facilitation expertise.

A small lady in large pink glasses—one of a London team who had braved the B7023 shortly after the worst snowstorm of the winter—began by explaining that, of course, only us folks here on the ground could solve the little problem we had probly kinda gotten to thinking of as a great big problem. But she and her team would help us find the strength and the tools to do a truly wonderful job.

After coffee, a few unlucky souls were singled out to stand up and say how they really felt about the problem. Real negative feelings, our facilitator explained, were OK. One of our most junior social workers even shed a tear or two. A briefing for the breakout sessions followed, the key to their success being the use of sticky notelets (“Yellow for happy thoughts, blue for bad thoughts—y'all know about the blues?”). An orthopod looked puzzled.

Over lunch a few disenchanted souls commented adversely on the morning's work, speculating that even Blue Peter and Billy Graham himself, working in perfect harmony, might not be quite up to solving bed blocking in Auchendreich.

The afternoon session was significantly less well attended, so at five to three Sir Hector—who has surprised even himself by surviving as our board chairman—closed the meeting and wished our guests a swift journey back to London, despite worrying news of further snowfalls, with drifting, on the B7023.
Dementia Improvement With Cytotoxic Chemotherapy (1997)

To describe a patient with Alzheimer disease and multiple myeloma (with Bence Jones proteinuria) with improvement of both conditions following cytotoxic chemotherapy, and to present issues related to the pathogenesis and management of Alzheimer disease.Report of a case.Progressive dementia and myeloma developed in a 64-year-old man. Cytotoxic chemotherapy for the myeloma resulted in maintained improvement of the dementia. Clinical, neuropsychological, pathological, and laboratory data are presented.For 2 years, vincristine sulfate, carmustine, melphalan, cyclophosphamide, and prednisone were administered cyclically, and the patient's dementia and lambda light chain production improved. Severe dementia recurred before death and cessation of therapy, although light chain production remained decreased.Dementia improvement with cytotoxic (ie, immunosuppressive, anti-inflammatory) therapy may offer insight into the pathogenesis and management of Alzheimer disease.
Safety and feasibility of the NanoKnife system for irreversible electroporation ablative treatment of canine spontaneous intracranial gliomas (2015)

With the incorporation of additional therapeutic planning procedures, the NanoKnife system is a novel technology capable of controlled IRE ablation of telencephalic gliomas.
Practical combinatorial (t-way) methods for detecting complex faults in regression testing (2011)

Regression testing can be among the most challenging of software assurance tasks because program changes often introduce faults, including unexpected interactions among different parts of the code. Unanticipated interactions may also occur when software is modified for a new platform. Techniques such as pairwise testing are not sufficient for detecting these faults, because empirical evidence shows that some errors are triggered only by the interaction of three, four, or more parameters. However, new algorithms and tools make it possible to generate tests that cover complex combinations of values (2-way to 6-way), or to analyze existing test suites and automatically generate tests that provide combinatorial coverage. The key advantage of this approach is that it produces better testing using a fraction of the tests required by other methods.
DNA Vaccination:  Transfection and Activation of Dendritic Cells as Key Events for Immunity (1999)

The mechanisms underlying initiation and maintenance of CD4 T cell responses after DNA vaccination were studied using a construct coding for nonsecreted fifth component of complement (C5) protein, thus restricting the availability of antigen. The only cell types to express C5 were keratinocytes at the site of DNA application and a small number of dendritic cells present in the draining lymph nodes. Antigen expression persisted for up to 12 wk in keratinocytes, but dendritic cells did not express C5 beyond 2 wk after vaccination. Cross-priming of dendritic cells by C5 expressed in keratinocytes did not occur unless keratinocyte death was induced by irradiation in vitro. CD4 T cells were activated in the draining lymph nodes only and subsequently migrated to the spleen, where memory T cells persisted for longer than 40 wk despite the absence of a source of persistent antigen. While DNA vaccination resulted in transfection of a small proportion of dendritic cells only, it led to general activation of all dendritic cells, thus providing optimal conditions for effective T cell activation and maintenance of memory.
Targeting, import, and processing of nuclear gene-encoded proteins into mitochondria and peroxisomes. (1990)

I have herein discussed two gene-enzyme families in maize whose protein products participate to purge toxic oxidants from cells, and are thus of importance to all aerobic organisms. We have demonstrated that plant mitochondria import precursor proteins (i.e., preSOD-3) in a manner analogous to other eukaryotic cells. The "transit peptide" (TP) of preSOD-3 is 31 amino acids long and has similar properties to other reported TPs for mitochondrial and chloroplastic proteins. Import to peroxisomes is uniquely different from that for mitochondria and chloroplasts in that no consensus presequence seems to be involved. Instead, targeting signals seem to be integral parts of peroxisomal proteins. These studies are important from a genetic engineering perspective in that it is not only important to express genes in specific tissues, but also within specific subcellular compartments. Expression of donor genes in appropriate cellular compartments of host cells will be critical for maintaining a stable, functional protein in the plant. Manipulation of metabolic pathways in plants requires that engineered proteins be efficiently and effectively expressed in the correct subcellular compartment and during the appropriate developmental time frame. The use of engineered proteins with altered topogenicity may not only prove useful in studying the role(s) of such proteins in metabolism, they may also serve to enhance our understanding of inter-organellar communication within the cell.
P1‐286: Using antisense oligonucleotides to knockdown endogenous brain tau in vivo (2012)

Alzheimer's Disease (AD), the most common form of dementia, is characterized by two pathological hallmarks: tau neurofibrillary tangles (NFT) and Aβ plaques. In addition, it has also been noted that AD is a clinical risk factor for late-onset seizures. Multiple AD mouse models recapitulate this increased seizure susceptibility and cognitive decline. Within the last 5 years, many of these AD models have been studied in the setting of mouse tau knockout (tau-/-). Both the increased seizure susceptibility and cognitive deficits were ameliorated in these amyloid-depositing lines when tau levels were reduced. Further, tau-/- alone interestingly appeared to be protective against chemically induced seizures. A similar knockout strategy, however, is unlikely to be practical in humans. To circumvent this, we propose using antisense oligonucleotide (ASO) technology to decrease endogenous tau expression levels in the adult mouse in an effort to study the effects on seizure susceptibility. ASOs targeted to mouse tau were infused into the lateral ventricle via osmotic pumps. To measure tau knockdown efficacy, mRNA and protein levels were measured by qRT-PCR and ELISA/Western Blot, respectively. To induce seizures, 55mg/kg of PTZ was injected intraparitoneally and the mouse allowed to seize for 15 minutes. We have identified a robust mouse tau knockdown oligo that effectively decreases total tau expression throughout the CNS of adult non-transgenic mice. As a test of whether tau knockdown has a functional effect in the adult model, we chemically induced seizures using PTZ and saw decreased seizure susceptibility in mice treated with the mouse tau knockdown oligo as compared to the vehicle or scrambled oligo control groups. Using ASOs that target mouse tau, we were able to effectively knockdown endogenous tau in the adult mouse. Of functional importance, mouse tau knockdown appeared to play the same protective role as mouse tau knockout in the setting of PTZ-induced seizures. This is the first time that tau knockdown in vivo has been shown to have a similar functional effect as tau knockout.
Design and implementation of 13C hyper polarization from para-hydrogen, for new MRI contrast agents (2005)

The order within proton pairs in organic molecules, resulting from hydrogenation with para-hydrogen, can be transferred in great part to nearby carbon-13 spins through adequate field manipulations. The molecules with hyperpolarized 13 C thus obtained can be used as new contrast agents of high efficiency in MRI. After a brief presentation of the hydrogenation process and apparatus, and a summary of the order transfer to the 13 C spins through field-cycling, we describe the use of radio-frequency irradiation, by CW and by pulses, to produce such an order transfer. We consider in turn the various steps involved, their rationale and their optimization. The final polarizations expected from both methods in the absence of relaxation are compared. Finally, some MRI pictures observed in vivo on animals are shown as an illustration of the contrast capacity of these methods. .
Proteinuria and Perinatal Lethality in Mice Lacking NEPH1, a Novel Protein with Homology to NEPHRIN (2001)

A high-throughput, retrovirus-mediated mutagenesis method based on gene trapping in embryonic stem cells was used to identify a novel mouse gene. The human ortholog encodes a transmembrane protein containing five extracellular immunoglobulin-like domains that is structurally related to human NEPHRIN, a protein associated with congenital nephrotic syndrome. Northern analysis revealed wide expression in humans and mice, with highest expression in kidney. Based on similarity to NEPHRIN and abundant expression in kidney, this protein was designated NEPH1 and embryonic stem cells containing the retroviral insertion in the Neph1 locus were used to generate mutant mice. Analysis of kidney RNA from Neph1−/− mice showed that the retroviral insertion disrupted expression of Neph1 transcripts. Neph1−/− pups were represented at the expected normal Mendelian ratios at 1 to 3 days of age but at only 10% of the expected frequency at 10 to 12 days after birth, suggesting an early postnatal lethality. The Neph1−/− animals that survived beyond the first week of life were sickly and small but without edema, and all died between 3 and 8 weeks of age. Proteinuria ranging from 300 to 2,000 mg/dl was present in all Neph1−/− mice. Electron microscopy demonstrated NEPH1 expression in glomerular podocytes and revealed effacement of podocyte foot processes in Neph1−/− mice. These findings suggest that NEPH1, like NEPHRIN, may play an important role in maintaining the structure of the filtration barrier that prevents proteins from freely entering the glomerular urinary space.