gebhart/patrepeval huggingface dataset:
Improved survival of lethally irradiated recipient mice transplanted with circulating progenitor cells mobilized by IL-8 after pretreatment with stem cell factor. (1996)

We have demonstrated previously that a single bolus-injection of interleukin (IL)-8 induces instant mobilization of hematopoietic progenitor cells (HPC) in mice and primates. To further improve the mobilization of HPC, we treated mice with hematopoietic growth factors (HGF) before IL-8-administration. The mobilized HPC were transplanted into lethally irradiated recipient mice to study the effects on survival. Male donor mice (age 8-12 weeks, weight 20-25 grams) were pretreated intraperitoneally (ip) with a fixed dose of 2.5 micrograms of either granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3, stem cell factor (SCF), or saline administered twice daily for 2 to 4 days. Then a fixed dose of 30 micrograms of IL-8 was administered ip at various time intervals before harvesting blood, bone marrow, and spleen. Cell counts and numbers of colony-forming units granulocyte/macrophage (CFU-GM) of these organs were assessed. Donor mice pretreated with HGF for 2 days and subsequently injected with IL-8 showed an increase in the numbers of circulating CFU-GM per mL blood from 168 +/- 98 to 402 +/- 201 (mean +/- SD, CFU-GM/mL blood) when GM-CSF was used, 314 +/- 133 to 2502 +/- 513 with G-CSF, and 27 +/- 15 to 524 +/- 339 with SCF compared with saline-pretreated controls (28 +/- 17 to 462 +/- 335 CFU-GM/mL blood, mean +/- SD; n = 42 and 40 per interval). Donor-mice pretreated for 4 days with IL-3 or GM-CSF showed an increase in the numbers of circulating HPC from 62 +/- 52 to 368 +/- 118 and 859 +/- 387 to 1034 +/- 421, respectively (CFU-GM/mL, mean +/- SD, n = 4 per group). Lethally irradiated (8.5 Gy) female Balb/c mice were then injected with decreasing numbers of peripheral blood mononuclear cells (PBMNC). Transplantation of 1.5 x 10(5) MNC obtained from donors pretreated with SCF for 2 days prior to IL-8 mobilization resulted in a significantly enhanced survival of 100% of the recipients, whereas recipients of PBM-NCs derived from donors treated with SCF only or IL-8 as a single injection had a survival rate at day 60 of only 50% and 60% respectively. When equal numbers of IL-8 mobilized MNCs from G-CSF, GM-CSF, or IL-3 pretreated donors were transplanted into lethally irradiated recipients, no such survival-advantage was observed. We conclude that pretreatment with SCF for 2 days improves the mobilizing effect induced by IL-8 and that transplantation of these cells enhances survival of lethally irradiated recipients.
Microfluidic cell trap array for controlled positioning of single cells on adhesive micropatterns (2012)

Adhesive micropattern arrays permit the continuous monitoring and systematic study of the behavior of spatially confined cells of well-defined shape and size in ordered configurations. This technique has contributed to defining mechanisms that control cell polarity and cell functions, including proliferation, apoptosis, differentiation and migration in two-dimensional cell culture systems. These micropattern studies often involve isolating a single cell on one adhesive protein micropattern using random seeding methods. Random seeding has been successful for isolated and, to a lesser degree, paired patterns, where two patterns are placed in close proximity. Using this method, we found that the probability of obtaining one cell per pattern decreases significantly as the number of micropatterns in a cluster increases, from 16% for paired micropatterns to 0.3% for clusters of 6 micropatterns. This work presents a simple yet effective platform based on a microfludic sieve-like trap array to exert precise control over the positioning of single cells on micropatterns. We observed a 4-fold improvement over random seeding in the efficiency of placing a pair of single cells on paired micropattern and a 40-fold improvement for 6-pattern clusters. The controlled nature of this platform can also allow the juxtaposition of two different cell populations through a simple modification in the trap arrangement. With excellent control of the identity, number and position of neighbouring cells, this cell-positioning platform provides a unique opportunity for the extension of two-dimensional micropattern studies beyond paired micropatterns to organizations containing many cells or different cell types.
Physical activity protects male patients with post-traumatic stress disorder from developing severe fibromyalgia. (2008)

Physical exercise in male patients with combat-related PTSD provides protection from the future development of fibromyalgia. Furthermore, physical activity is related in this group of patients to a better perception of their quality of life.
Targeted disruption of the activating transcription factor 4 gene results in severe fetal anemia in mice (2002)

Activating transcription factor (ATF) 4 is a ubiquitous basic leucine-zipper transcription factor that is a member of the ATF/cyclic adenosine monophosphate responsive element-binding (CREB) protein family. To determine the in vivo function of ATF4, the ATF4 gene in murine embryonic stem cells was deleted and homozygous mutant mice were generated. ATF4 null fetuses were severely anemic because of an impairment in fetal-liver definitive hematopoiesis; the hematocrit in 15.5-day mutant fetuses was 0.15, whereas that in controls was 0.35. The fetal livers in homozygous ATF4 mutants were pale and hypoplastic. In vitro culture of fetal-liver cells showed fewer hematopoietic progenitors per embryo and a dramatic decrease in the size of progenitor colonies. Culture of primary murine embryonic fibroblasts showed a proliferative defect. These results suggest that ATF4 is critical, in a cell-autonomous manner, for normal cellular proliferation, especially for the high-level proliferation required during fetal-liver hematopoiesis.
Treatment of severe seasonal rhinoconjunctivitis by a combination of azelastine nasal spray and eye drops: a double-blind, double-placebo study. (2001)

Combining azelastine eye drops and nasal spray is a safe and effective treatment of severe seasonal rhinoconjunctivitis.
Acute Myocardial Infarction in Sickle Cell Disease (2008)

Myocardial infarction in young adults is, in practice, a diagnosis of exclusion. Given the fact that most of the patients with sickle cell disease are young and have predisposition to painful crisis, they are often overlooked for myocardial infarction. These patients often have few or no traditional risk factors for coronary artery disease, and risk stratification tools such as the Thrombolysis in Myocardial Infarction (TIMI) and Global Registry of Acute Coronary Events (GRACE) models place these patients at low risk. Nonspecific changes on electrocardiogram are of little diagnostic value. Myocardial infarction is very often a missed diagnosis in patients with sickle cell disease. Diagnostic criteria, potential mechanisms, and management for acute myocardial infarction in patients with sickle cell disease are discussed.
Wave-field transformations of vertical seismic profiles (1987)

Abstract Vertical seismic profile (VSP) data may be partitioned in a variety of ways by application of wave-field transformations. These transformations provide insights into the nature of the data and aid in the design of processing operations. Transformations are implemented in a reversible sequence that takes the observed VSP data from the depth-time (z-t) domain through the slowness-time intercept (p-tau ) domain (by a slant stack), to the slowness-frequency (p-omega ) domain (by a 1-D Fourier transform over tau ), to the wavenumber-frequency (k-omega ) domain (by resampling using the Fourier central-slice theorem), and finally back to the z-t domain (by an inverse 2-D Fourier transform).Multidimensional wave-field transformations, combined with k-omega , p-omega , and p-tau filtering, can be applied to wave-field resampling, interpolation, and extrapolation; separation of P-waves and S-waves; separation of upgoing and downgoing waves; and wave-field decomposition for isolation, identification, and analysis of arrivals.
Urban models texturing from un-calibrated photographs (2008)

We present a system to automatically fit a CAD urban model to a structure from motion sparse reconstruction. Fitting the model to the sparse reconstruction is defined and solved as an absolute orientation problem. The novelty of our approach is the use of facade correspondences to compute the absolute orientation. We also propose a solution to automatically establish facade correspondences using a pseudo-RANSAC procedure. Our system consists of four parts: a robust structure from motion recovery framework able to register accurately a set of digital photographs, a method to recover the principal walls of a scene from a 3d sparse reconstruction using a robust RANSAC scheme, a method to fit automatically a 3d model to a set of 3d wall planes, and finally, a user interface allowing scene visualisation and photorealistic rendering of urban 3d models from a set of calibrated photographs.
Urinary and metabolic clearances of arginine vasopressin in normal subjects (1986)

Synthetic arginine vasopressin (AVP) was infused into 11 hydrated normal subjects at five different infusion rates ranging from 10 to 350 microU X kg-1 X min-1. Each infusion rate was continued for 1 h, and urinary determinations were made on the 30- to 60-min specimens during which time there was no further rise in plasma AVP. Urinary AVP concentrations (microU/ml) and excretion rates (microU/min) increased linearly with increasing infusion rates, and the concentration of AVP in urine increased 120 times more rapid than plasma. Urinary and metabolic clearances of AVP also increased linearly with the maximum urinary clearance being 60.6% of the creatinine clearance. The total metabolic clearance of AVP (including urinary clearance) was 17.8 times that of the urinary clearance of AVP alone. These data clarify the relationships between plasma and urinary AVP in normal hydrated subjects during AVP infusion under steady-state conditions and emphasize the potential advantage of measuring urinary AVP as a monitor of endogenous AVP secretion.
Controlled thyristor turn-on for high DI/DT capability (1981)

In a one or more amplified stage thyristor design it is possible to control the peak current level of all but the final stage with impedance built into the p-base zone. This impedance reduces both the current and the duty cycle of the protected amplifying stage effectively protecting it from undesirable temperature rises during turn-on. A further bonus and perhaps equally important is the fact that the amplifying stage and its current control impedance can be used to reduce and essentially fix the voltage level at which the following stage turns on, This results in a lower voltage, lower stress turn-on of the following stage and a device essentially protected from di/dt turn-on failure. This paper describes several aspects of controlled turn-on in the context of a 2.6KV and 5KV light triggered thyristor. In particular we discuss selection of the resistor value, the problem of unwanted current control resistor modulation by device current as well as some factors affecting the proper wattage of such resistors. We also discuss the role current control resistors can play in controlling avalanche current from known locations on the device.
Ambient Template‐Directed Synthesis of Single‐Crystalline Alkaline‐Earth Metal Fluoride Nanowires (2006)

A facile and mild means of controllably preparing a series of single-crystalline alkaline-earth metal fluoride nanowires of reproducible shape and varying sizes (see figure) using a simple, room-temperature approach has been developed. This approach is based on the use of readily commercially available polycarbonate template membranes. The favorable luminescence properties of the doped nanowires imply their possible incorporation into functional nanoscale devices.
Too Hot to Handle: The Race for Cold Fusion (1992)

Frank Close, a leading physicist and talented popular science writer, reveals the true story of the cold fusion controversy--a story ignored until now in spite of the glare of publicity surrounding Martin Fleischmann and Stanley Pons. On March 23, 1989, these two Utah scientists held an astonishing press conference, maintaining that they had succeeded, working in secret, in harnessing atomic fusion. What was the basis for their claims to have achieved cold fusion in a test tube in a basement laboratory, while other scientists--using magnets as big as houses and temperatures hotter than those in the center of the sun--were failing to produce as much power as they were using? Why did Fleischmann and Pons proclaim their discovery at a news conference, when first announcements of scientific results are almost always made within the scientific community? Why did the full-blown media event inspired by their initial report cause governments to reorient their research programs in hopes of cornering the new technology? And why did some scientists recklessly abandon their traditional painstaking methods in haste to be first to prove or discredit the experiment? Acquainted at first hand with investigations of cold fusion on two continents, Close is uniquely qualified to probe the motivations behind Fleischmann's and Pons's startling assertions and to explore the intellectual and political turmoil that surrounded the cold fusion debate. Originally published in 1991. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
A novel intestinal trans-factor (NF-LPH1) interacts with the lactase-phlorizin hydrolase promoter and co-varies with the enzymatic activity. (1992)

The promoter of the pig lactase-phlorizin hydrolase was cloned and showed to be functional in the human intestinal cell line Caco2. The proximal promoter was analyzed for binding of nuclear proteins from small intestine and liver. DNase I footprinting and electrophoretic mobility shift assays show, that an intestinal nuclear factor (NF-LPH1) binds to a sequence (-40 to -54) located close to the TATA-box. Enterocytes from newborn pigs with high lactase activity contain high amounts of NF-LPH1, whereas enterocytes from adult pigs with low lactase activity contain low amounts of NF-LPH1. The liver does not contain lactase activity, and NF-LPH1 is not present in liver nuclear extracts in detectable amounts. This indicates that NF-LPH1 is involved in the decline of lactase at weaning and may be of importance for the molecular explanation of hypolactasia in humans. It was demonstrated by transfection of two different promoter-reporter gene constructs into Caco2 cells, that there are additional cis-element(s) in the region -142 to approximately -980, which are important for the transcription of the lactase-phlorizin hydrolase gene.
Nitric oxide transport on sickle cell hemoglobin: Where does it bind? (2001)

We have recently reported that nitric oxide inhalation in individuals with sickle cell anemia increases the level of NO bound to hemoglobin, with the development of an arterial-venous gradient, suggesting delivery to the tissues. A recent model suggests that nitric oxide, in addition to its well-known reaction with heme groups, reacts with the beta-globin chain cysteine 93 to form S-nitrosohemoglobin (SNO-Hb) and that SNO-Hb would preferentially release nitric oxide in the tissues and thus modulate blood flow. However, we have also recently determined that the primary NO hemoglobin adduct formed during NO breathing in normal (hemoglobin A) individuals is nitrosyl (heme)hemoglobin (HbFeIINO), with only a small amount of SNO-Hb formation. To determine whether the NO is transported as HbFeIINO or SNO-Hb in sickle cell individuals, which would have very different effects on sickle hemoglobin polymerization, we measured these two hemoglobin species in three sickle cell volunteers before and during a dose escalation of inhaled NO (40, 60, and 80 ppm). Similar to our previous observations in normal individuals, the predominant species formed was HbFeIINO, with a significant arterial-venous gradient. Minimal SNO-Hb was formed during NO breathing, a finding inconsistent with significant transport of NO using this pathway, but suggesting that this pathway exists. These results suggest that NO binding to heme groups is physiologically a rapidly reversible process, supporting a revised model of hemoglobin delivery of NO in the peripheral circulation and consistent with the possibility that NO delivery by hemoglobin may be therapeutically useful in sickle cell disease.
Distribution, Density, and Diversity of Heterodera glycines in Missouri. (1993)

Between May, 1988, and July, 1992, the University of Missouri Plant Nematode Diagnostic Laboratory collected data on Heterodera glycines populations in Missouri by offering diagnostic services to soybean farmers. A greenhouse bioassay (free), egg count ($10/sample), and race determination test ($25) were conducted by request on soil samples submitted to the lab. Each test was offered for a specific purpose: the bioassay for fields not known to be infested; the egg count for population monitoring in fields with known infestations; and the race test for fields with a history of resistant cultivars. Of 818 samples submitted for bioassay on a H. glycines-susceptible soybean cultivar, 13 (1.6%) contained brown cysts but no white females, and 364 (45%) contained white females after 35 days in the greenhouse. Of 6,193 egg counts, 39% were either free of H. glycines or contained fewer than 500 eggs/250 cm(3) soil, the action threshold for Missouri. The remaining 61% ranged from 500-400,000 eggs/250 cm(3) soil (mean = 10,617). Eleven races were detected, with races 3 (45%) and 1 (23%) the most common. The data show that H. glycines is widespread in Missouri (with confirmed infestations in 80 of 114 counties), that most infested fields have population densities above the action threshold, and that there is considerable genetic diversity among H. glycines field populations.
Immunocytochemical Methods and Protocols (1999)

Lorette Javois' timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research p
Isolation of hepatoblasts based on the expression of Dlk/Pref-1 (2003)

Hepatoblasts are common progenitors for hepatocytes and biliary epithelial cells, although their nature remains largely unknown. In order to isolate and to characterize hepatoblasts, we searched for cell surface antigens expressed in mouse fetal hepatic cells by the signal sequence trap method and found that Dlk, also known as Pref-1, was strongly expressed in fetal liver. Immunohistochemical as well as northern analysis indicated that Dlk was highly expressed in the E10.5 liver bud. The strong expression continued until the E16.5 stage and was significantly downregulated thereafter. Using a monoclonal antibody against Dlk, we isolated Dlk+ cells either by a fluorescence-activated cell sorter or by an automatic magnetic cell sorter. Dlk+ cells isolated from fetal livers expressed albumin and formed colonies when cultured at low density with HGF and EGF for 5 days. Over 60% of colonies derived from E14.5 Dlk+ cells contained both albumin+ and cytokeratin 19+ cells, indicating that a majority of colony-forming Dlk+ cells are able to differentiate into both hepatocyte and biliary epithelial cell lineages. In addition, numerous microvilli were observed by electronmicroscopic analysis in most of those cultured cells, also indicating differentiation of Dlk+ cells under this condition. Furthermore, 7% of the colony-forming Dlk+ cells were not only bipotential but also highly proliferative, forming a large colony containing more than 100 cells during 5 days of culture. By transplantation of Dlk+ cells into the spleen, donor-derived hepatocytes were found in the recipient liver, indicating that Dlk+ cells differentiated into hepatocytes in vivo. These results indicate that Dlk+ cells are hepatoblasts and that Dlk is a useful marker to enrich highly proliferative hepatoblasts from fetal liver.
Systemic Complement Activation Produces Hemodynamic Changes Characteristic of Sepsis (1988)

Zymosan was administered intravenously in graded doses to Sprague-Dawley rats to investigate the hemodynamic effects of systemic complement activation. At two hours, thermodilution cardiac output, mean arterial pressure, heart rate, systemic vascular resistance, hematocrit, effective hepatic and renal blood flows, and percent change in total hemolytic complement activity were measured on all animals. Progressively increasing doses of zymosan produced characteristic hemodynamic changes of progressively deteriorating stages of hyperdynamic sepsis. In addition, complement activation resulted in a redistribution of systemic blood flow with hepatic hypoperfusion similar to that seen in sepsis. Renal blood flow was unaffected early after complement activation. Additional rats were studied from the control and a representative zymosan-treated group at 24 and 48 hours to determine if the hemodynamic changes observed at two hours persisted or resolved. All systemic hemodynamic measures returned to normal at both 24 and 48 hours. Liver blood flow, however, remained depressed and actually deteriorated over time. Renal perfusion, which was stable at both two and 24 hours, fell below control values in the zymosan-treated group at 48 hours. We conclude that complement may be a mediator of both systemic and visceral flow abnormalities that precede, and perhaps precipitate, organ failure in trauma and sepsis.
The High Cost of Poor Communication (1994)

Communication potential abounds: e‐mail zips from office to office; electronic calendars search a building of people, coming up with a common date, and scheduling it for the masses; acquaintances from across the state or nation no longer play telephone tag—they “talk” at their convenience via the Internet. But are we really communicating? Are we breaking down barriers or constructing more substantial ones—ones that are being made impenetrable because we no longer are able subconsciously to analyze body language or casually change the subject when tensions mount?
Screening for Lung Cancer: U.S. Preventive Services Task Force Recommendation Statement (2013)

The USPSTF recommends annual screening for lung cancer with low-dose computed tomography in adults aged 55 to 80 years who have a 30 pack-year smoking history and currently smoke or have quit within the past 15 years. Screening should be discontinued once a person has not smoked for 15 years or develops a health problem that substantially limits life expectancy or the ability or willingness to have curative lung surgery. (B recommendation).