gebhart/patrepeval huggingface dataset:
Antiproliferative Effect of IL-1 Is Mediated by p38 Mitogen-Activated Protein Kinase in Human Melanoma Cell A375 (1999)

The role of p38 mitogen-activated protein kinase (MAPK) in IL-1-induced growth inhibition was investigated using IL-1-sensitive human melanoma A375-C2-1 cells and IL-1-resistant A375-R8 cells. In both cells, p38 MAPK was activated by IL-1. A selective inhibitor for p38 MAPK, SB203580, almost completely recovered the IL-1-induced growth inhibition in A375-C2-1 cells. IL-1-induced IL-6 production was also suppressed by SB203580. However, the reversal effect of SB203580 was not due to the suppression of IL-6 production because the SB203580 effect was still observed in the presence of exogenous IL-6. Down-regulation of ornithine decarboxylase (ODC) activity as well as its protein level has been shown to be essential for IL-1-induced growth inhibition. SB203580 also reversed the IL-1-induced down-regulation of ODC activity and intracellular polyamine levels without affecting ODC mRNA levels in A375-C2-1 cells. In IL-1-resistant R8 cells, however, IL-1 only slightly suppressed ODC activity. In A375-C2-1 cells, the mRNA expression level of antizyme (AZ), a regulatory factor of ODC activity, has been shown to be up-regulated by IL-1. IL-1-induced up-regulation of AZ mRNA level was not affected by SB203580. These findings demonstrate that p38 MAPK plays an important role in IL-1-induced growth inhibition in A375 cells through down-regulating ODC activity without affecting the level of ODC mRNA and AZ mRNA. In IL-1-resistant A375-R8 cells, IL-1 signaling pathway is deficient between p38 MAPK activation and down-regulation of ODC activity.
A Single Amino Acid Mutation in the Carnation Ringspot Virus Capsid Protein Allows Virion Formation but Prevents Systemic Infection (2001)

A Carnation ringspot virus (CRSV) variant (1.26) was identified that accumulates virions but is incapable of forming a systemic infection. The 1.26 capsid protein gene possesses a Ser-->Pro mutation at amino acid 282. Conversion of 1.26 amino acid 282 to Ser restored systemic infection, while the reciprocal mutation in wild-type CRSV abolished systemic infection. Similar mutations introduced into the related Red clover necrotic mosaic virus capsid protein gene failed to induce the packaging but nonsystemic movement phenotype. These results provide additional support for the theory that virion formation is necessary but not sufficient for systemic movement with the dianthoviruses.
Ring-Fusion of Perylene Diimide Acceptor Enabling Efficient Nonfullerene Organic Solar Cells with a Small Voltage Loss (2017)

We report a novel small molecule acceptor (SMA) named FTTB-PDI4 obtained via ring-fusion between the thiophene and perylene diimide (PDI) units of a PDI-tetramer with a tetrathienylbezene (TTB) core. A small voltage loss of 0.53 V and a high power conversion efficiency of 10.58% were achieved, which is the highest value reported for PDI-based devices to date. By comparing the fused and nonfused SMAs, we show that the ring-fusion introduces several beneficial effects on the properties and performances of the acceptor material, including more favorable energy levels, enhanced light absorption and stronger intermolecular packing. Interestingly, morphology data reveal that the fused molecule yields higher domain purity and thus can better maintain its molecular packing and electron mobility in the blend. Theoretical calculations also demonstrate that FTTB-PDI4 exhibits a "double-decker" geometry with two pairs of mostly parallel PDI units, which is distinctively different from reported PDI-tetramers with highly twisted geometries and can explain the better performance of the material. This work highlights the promising design of PDI-based acceptors by the ring-fusion strategy.
Spectrum of Seasonal Allergic Rhinitis Symptom Relief with Topical Corticoid and Oral Antihistamine Given Singly or in Combination (1996)

Sixty ragweed-sensitive volunteers participated in a 2-week study that compared symptom profiles during treatment with antihistamine (loratadine, LOR) alone, topical corticoid (beclomethasone, BEC) alone, or the two drugs combined. For 5 days commencing shortly after the beginning of the ragweed bloom, patients took no treatment while we collected baseline data. They were then randomized to one of the three treatments, receiving that treatment for the balance of the 2-week study term. Twice each day they recorded the severity of congestion, eye symptoms, running and blowing, itching, and sneezing. At the end of the study they provided an estimate of overall symptom relief, which favored combined treatment (vs LOR P = 0.001, vs BEC P = 0.042). To gain an estimate of disease severity and treatment effectiveness over time, and to smooth out day-to-day variation, we divided symptom diary reports into three segments (days 2–4, 5–7, and 8–10) for analysis. Combined treatment controlled symptoms better than antihistamine alone in nearly all study segments. Corticoid alone or combined with antihistamine provided similar control of congestion, running and blowing, and eye complaints. Combination therapy controlled itching and sneezing better, especially through the study segments 1 and 2. Patient preference for combined treatment seems to relate to control of itching and sneezing and rapid onset of effect.
Insulin immunization of nonobese diabetic mice induces a protective insulitis characterized by diminished intraislet interferon-gamma transcription. (1995)

We reported previously that daily injections of isophane insulin prevented both hyperglycemia and insulitis in nonobese diabetic (NOD) mice (Atkinson, M., N. Maclaren; and R. Luchetta. 1990. Diabetes. 39:933-937). The possible mechanisms responsible include reduced immunogenicity of pancreatic beta-cells from "beta-cell rest" and induced active immunoregulation to insulin (Aaen, IK., J. Rygaard, K. Josefsen, H. Petersen, C. H. Brogren, T. Horn, and K. Buschard. 1990. Diabetes. 39:697-701). We report here that intermittent immunizations with insulin or its metabolically inactive B-chain in incomplete Freund's adjuvant also prevent diabetes in NOD mice, whereas immunizations with A-chain insulin or with BSA do not. Adoptive transfer of splenocytes from B-chain insulin-immunized mice prevented diabetes in recipients co-infused with diabetogenic spleen cells, an effect that was abolished by prior in vivo elimination of either CD4+ or CD8+ cells. Insulin immunization did not reduce the extent of intraislet inflammation (insulitis); however, it did abolish expression of IFN-gamma mRNA within the insulitis lesions. Immunizations with insulin thus induce an active suppressive response to determinants on the B-chain that converts the insulitis lesion from one that is destructive to one that is protective.
Oral levodopa dose‐response study in MPTP‐induced hemiparkinsonian monkeys: Assessment with a new rating scale for monkey parkinsonism (1991)

Quantitative measures for the severity of MPTP-induced parkinsonism and response to antiparkinsonian interventions in monkeys have been lacking. We carried out an oral levodopa dose-response study in two rhesus monkeys whose left hemiparkinsonism was induced by intracarotid administration of MPTP. A newly developed clinical rating scale of monkey parkinsonism showed a consistent dose-response relationship for levodopa over the dosage range of 50-3,500 mg/day. Antiparkinsonian effects appeared at 200 mg/day and were optimal at 1,000-2,000 mg/day. Levodopa also reversed rotational behavior, improved movement times for both the impaired and opposite upper limb, and produced dyskinesias at high dosages. Thus, MPTP-induced hemiparkinsonism in monkeys closely resembles the human disease condition, is associated with sensitive response measures, and should prove valuable for assessing novel antiparkinsonian therapies.
CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. XVII. A NOTE ON THE ABSORPTION RATES OF FIBRIN FILMS IN TISSUE 12 (1944)

The most effective surgical use of fibrin film (1) or foam (2) demands some knowledge of their rates of absorption in the body. Fundamental observations on the histological sequences involved in the course of absorption of fibrinogen plastics (1) implanted in tissue have been made by Bailey, Ford, and Hawn (3). The present study was undertaken to measure the rates of absorption of various types of fibrin films, so that it might be possible to adjust the persistence of film in the body to meet specific requirements. MATERIALS AND METHODS The fibrin film used in these experiments was that de-scribed in the preceding paper (1), prepared from human fibrinogen (4, 5). It was subjected to varying degrees of modifying treatment before implantation. Squares of film (1 cm. X 1 cm.) were then implanted subcutaneously and intermuscularly in rabbits and held in place by silk sutures. In order to determine the initial fibrin content of the im-planted specimens, similar pieces of film were soaked in distilled water to free them of water-soluble substances, dried at 1100 C., and weighed to 0.3 mgm. This method was reproducible within 5 per cent. Three different weights of film were employed, with 4, 9, and 11 mgm. fibrin per sq.cm. The final fibrin content of the films was determined after different periods of implantation by sub-mitting the remains of the films to the soaking, drying, and weighing procedure described above. In cases where en-capsulation occurred, the weight of the capsule was meas-ured separately after soaking and drying. Whereas the film may be easily recovered after brief periods of implan-tation, it becomes increasingly difficult to identify as ab-sorption proceeds, owing to fragmentation and possible 1This work has been carried out under contract, recom-
Measurement of pharyngeal transit time by electrical impedance tomography (1992)

The feasibility of using electrical impedance tomography (EIT) for measuring pharyngeal transit time has been investigated. Imaging sequences were obtained on the neck in a group of eight healthy subjects when swallowing solutions of different electrical conductivities. The solutions used were aqueous Dioralyte (1.5 S m-1) and tap water (0.04 S m-1). The effects of movement upon the images were large compared with the changes due to the solutions, but these were cancelled by a subtraction technique. The mean value of the EIT pharyngeal transit time parameter was 370 +/- 120 ms. EIT has the advantage that repeated measurements could be performed without fear of hazard in contrast to the established technique of video fluoroscopy which involves ionising
Role of Endogenous Glucagon-Like Peptide-1 in Islet Regeneration After Partial Pancreatectomy (2003)

A reduction in beta-cell mass is an important causative factor in type 1 and type 2 diabetes. Glucagon-like peptide-1 (GLP-1) and the long-acting agonist exendin 4 (Ex-4) expand beta-cell mass by stimulating neogenesis and proliferation. In the partial pancreatectomy (Ppx) model, exogenous Ex-4 promotes islet regeneration, leading to sustained improvement in glucose tolerance. In this study, we investigate the potential role of endogenous GLP-1 in islet growth. We examined beta-cell mass regeneration after 70% Ppx in mice receiving the GLP-1 antagonist Ex9-39 and in GLP-1R(-/-) mice. In Ex9-39-treated sham-operated mice, persistent fasting hyperglycemia was observed, but beta-cell mass was not diminished. In pancreatectomized mice, persistent glucose intolerance was noted, but this was not further exacerbated by Ex9-39. Accordingly, beta-cell mass recovery of Ppx mice was not impaired by Ex9-39. In contrast, GLP-1R(-/-) CD1 mice showed worse glucose intolerance after Ppx compared with wild-type CD1 Ppx mice, and this correlated with a significant defect in beta-cell mass regeneration. The recovery of beta-cell mass differed markedly in the BALB/c and CD1 control mice, indicating a significant role of genetic background in the regulation of beta-cell mass. These studies point to a role for endogenous GLP-1 in beta-cell regeneration after Ppx in mice.
Unilateral frame distraction: proximal tibial valgus osteotomy for medial gonarthritis. (1993)

An undertreated group has emerged in modern orthopaedic surgery--patients with degenerative disease of the medial compartment of the knee who are young and active, who may have undergone multiple arthroscopic procedures, and who have been advised to "take it easy and wait for a total knee replacement." If the knee is in varus, unilateral frame distraction with upper tibial corticotomy is advocated to achieve valgus alignment. The procedure is straightforward, with few complications in our first 100 cases, with almost universal success. Even if it is believed that all tibial osteotomies will eventually fail and require a total knee replacement, this method, by which bone stock is actually enhanced, length is maintained, and the ligamentous structures about the knee are undamaged, is superior to methods previously described.
Requirement for Cell Division for Initiation of Transcription of Rous Sarcoma Virus RNA (1974)

Stationary chicken embryo fibroblasts exposed to Rous sarcoma virus (RSV) did not synthesize virus RNA until after division of the infected cells. Initiation of RNA transcription after cell division resulted in transcription of the entire viral genome and was followed shortly thereafter by appearance of progeny virus. Addition of colchicine to RSV-infected stationary chicken embryo fibroblasts resulted in inhibition of cell division and a 24-h delay in the appearance of virus RNA. RSV-infected chicken embryo fibroblasts made stationary 10 to 15 cell generations after infection continued to produce an amount of progeny virus similar to that produced in dividing RSV-infected cells. Teh virus produced from cells made stationary after virus production had begun contained RNA synthesized in stationary cells. Therefore, division of the RSV-infected chicken embryo fibroblast is required for the initiation of transcription of virus RNA, but it is not required for the maintenance of transcription of virus RNA.
Cash management decision with probability criterion (2002)

A mathematical model for the problem of multiperiod cash management is established. The problem is based on a discrete stochastic dynamic system and the decision criterion is a probability criterion. It is to maximize the probability that the investment gain rate is no less than a given expected gain rate in every period. Since this problem is an optimal control problem of a discrete stochastic system and more complex than the classical optimal control problem, the criterion function can not be calculated by analytic formulation. The traditional solving techniques are no longer valid. In this paper, a method of computing the value of the criterion function by stochastic simulation is presented, and a method based on the genetic algorithm for solving this multiperiod cash management problem is provided. Finally, the steps of the stochastic simulation based genetic algorithm are listed and an illustration example is given.
Low-molecular-weight variants of osteopontin generated by serine proteinases in urine of patients with kidney stones (1996)

Osteopontin (OPN) is a multifunctional glycosylated phosphoprotein found in body fluids, including urine, and has been implicated in urinary stone formation. We tested the hypothesis that OPN levels in urine of patients with kidney stones differed from normal individuals. To quantify OPN levels in the urine, we developed an ELISA using a combination of a mouse monoclonal and rabbit polyclonal antibodies raised against a recombinant glutathione-S-transferase-human OPN fusion protein. In a group of 34 patients diagnosed with kidney stones compared with a control group of 23 normal individuals, we found that OPN levels in urine of the patient and control groups ranged from 0.01 to 2.7 micrograms/ml, with no significant difference in their medians (P > 0.8, Mann-Whitney test). OPN in urine was qualitatively assessed by Western blotting using a biotinylated monoclonal antibody to detect various molecular forms. The urine of most individuals contained OPN species within in the 55- to 66-kDa electrophoretic mobility range. However, a significantly higher proportion of individuals in the patient group (13 of 34) was found to have aberrant urine OPN species (< or = 40 kDa) compared to 2 of 23 for the control group (P < 0.03, chi 2 test). Mixing experiments indicated that urine samples with aberrant OPN contain proteases inhibitable with phenylmethylsulfonyl fluoride. Such proteases could break down normal urine OPN in vitro. Therefore, urine from a high frequency of kidney stone patients contains serine proteases that contribute to proteolytic cleavage of OPN.
Abstraction techniques for validation coverage analysis and test generation (1998)

The enormous state spaces which must be searched when verifying the correctness of, or generating tests for, complex circuits precludes the use of traditional approaches. Hard-to-find abstractions are often required to simplify the circuits and make the problems tractable. This paper presents a simple and automatic method to extract the control flow of a circuit so that the resulting state space can be explored for validation coverage analysis and automatic test generation. This control flow, capturing the essential "behavior" of the circuit, is represented as a finite state machine called the ECFM (Extracted Control Flow Machine). Simulation is currently the primary means of verifying large circuits, but the definition of a coverage measure for simulation vectors is an open problem. We define functional coverage as the amount of control behavior covered by the test suite. We then combine formal verification techniques, using BDDs as the underlying representation, with traditional ATPG techniques to automatically generate additional sequences which traverse uncovered parts of the control state graph. We also demonstrate how the same abstraction techniques can complement ATPG techniques when attacking hard-to-detect faults in the control part of the design for which conventional ATPG alone proves to be inadequate or inefficient at best. Results on large designs show significant improvement over conventional algorithms.
Antimicrobial Peptide Therapeutics for Cystic Fibrosis (2005)

Greater than 90% of lung infections in cystic fibrosis (CF) patients are caused by Pseudomonas aeruginosa, and the majority of these patients subsequently die from lung damage. Current therapies are either targeted at reducing obstruction, reducing inflammation, or reducing infection. To identify potential therapeutic agents for the CF lung, 150 antimicrobial peptides consisting of three distinct structural classes were screened against mucoid and multidrug-resistant clinical isolates of P. aeruginosa, Stenotrophomonas maltophilia, Achromobacter xylosoxidans, and Staphylococcus aureus. Five peptides that retained potent antimicrobial activities in physiological salt and divalent cation environment were further characterized in vivo using a rat chronic lung infection model. All animals were inoculated intratracheally with 10(4) P. aeruginosa mucoid PAO1 cells in agar beads. Three days following inoculation treatment was initiated. Animals were treated daily for 3 days with 100 microl of peptide solution (1 mg/ml) in 10 mM sodium citrate, which was deposited via either intratracheal instillation or aerosolization. Control animals received daily exposure to vehicle alone. At the end of the treatment the lungs of the animals were removed for quantitative culture. Four peptides, HBCM2, HBCM3, HBCPalpha-2, and HB71, demonstrated significant reduction in Pseudomonas bioburden in the lung of rats. Further in vivo studies provided direct evidence that anti-inflammatory activity was associated with three of these peptides. Therefore, small bioactive peptides have the potential to attack two of the components responsible for the progression of lung damage in the CF disease: infection and inflammation.
Integral sunshade for an optical reception antenna (1991)

Optical reception antennas (telescopes) must be capable of receiving communications even when the deep-space laser source is at a small solar elongation (appears from Earth to be located within a small angle of the Sun.) Direct sunlight must not be allowed to shine on the primary reflector of an optical reception antenna, because too much light would be scattered into the signal detectors. A conventional sunshade that does not obstruct the antenna aperture would have to be about five times longer than its diameter for reception at a solar elongation of 12 deg. Such a long sunshade is unwieldy, either on the ground or in space. Since a new large-aperture optical reception antenna will probably have a hexagonally segmented primary reflector, a sunshade consisting of closely packed hexagonal tubes is proposed. It will be mounted in alignment with the segmentation to avoid producing any additional geometric obstruction. The tubes will be extended downward toward the primary reflector, until they reach the envelope of the focused beam to the secondary reflector. When the optical reception antenna is ground based, the other ends of the tubes will be trimmed and the antenna will be mounted so that both the sunshade and the antenna will swing within a sphere whose diameter is only six-fifths the diameter of the primary reflector. If the segmentation involves four rings of hexagons around an absent central hexagon, then this sunshade will be useful when the solar elongation is as small as 12 deg. The usefulness can be extended to 10 deg by adding shutters to block the tube corners that admit off-axis sunlight. Vanes or slats may be inserted in the tubes to permit operation at 6 deg, or double vanes down to 3 deg. The structure of the sunshade is very strong and may be used to support the secondary reflector instead of an independent support.
Inhibition of thymidine kinase by P1-(adenosine-5‘)-P5-(thymidine-5‘)-pentaphosphate. (1986)

Potential bisubstrate analogs, with adenosine and thymidine joined at their 5' positions by polyphosphoryl linkages of varying lengths (ApndT, where n = the number of phosphoryl groups), were examined as inhibitors of cytosolic thymidine kinase from blast cells of patients with acute myelocytic leukemia. Ki values were 1.2 microM for Ap3dT, 0.31 microM for Ap4dT, 0.12 microM for Ap5dT, and 0.19 microM for Ap6dT. The best inhibitor of the cytosolic enzyme, Ap5dT, was somewhat less effective as an inhibitor of the mitochondrial enzyme (Ki = 0.50 microM). In addition to their inhibitory modes of binding by the cytosolic enzyme, these compounds were bound at considerably lower concentrations (Kd = 0.029 microM for Ap4dT, 0.0025 microM for Ap5dT, and 0.0027 microM for Ap4dT), in such a way as to protect the cytosolic enzyme from thermal inactivation at 37 degrees C in the absence of substrates.