gebhart/patrepeval huggingface dataset:
Effective scattering coefficient of the cerebral spinal fluid in adult head models for diffuse optical imaging (2006)

An efficient computation of the time-dependent forward solution for photon transport in a head model is a key capability for performing accurate inversion for functional diffuse optical imaging of the brain. The diffusion approximation to photon transport is much faster to simulate than the physically correct radiative transport equation (RTE); however, it is commonly assumed that scattering lengths must be much smaller than all system dimensions and all absorption lengths for the approximation to be accurate. Neither of these conditions is satisfied in the cerebrospinal fluid (CSF). Since line-of-sight distances in the CSF are small, of the order of a few millimeters, we explore the idea that the CSF scattering coefficient may be modeled by any value from zero up to the order of the typical inverse line-of-sight distance, or approximately 0.3 mm(-1), without significantly altering the calculated detector signals or the partial path lengths relevant for functional measurements. We demonstrate this in detail by using a Monte Carlo simulation of the RTE in a three-dimensional head model based on clinical magnetic resonance imaging data, with realistic optode geometries. Our findings lead us to expect that the diffusion approximation will be valid even in the presence of the CSF, with consequences for faster solution of the inverse problem.
Cross-clade neutralization of primary isolates of human immunodeficiency virus type 1 by human monoclonal antibodies and tetrameric CD4-IgG (1995)

We have tested three human monoclonal antibodies (MAbs) IgG1b12, 2G12, and 2F5) to the envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1), and a tetrameric CD4-IgG molecule (CD4-IgG2), for the ability to neutralize primary HIV-1 isolates from the genetic clades A through F and from group O. Each of the reagents broadly and potently neutralized B-clade isolates. The 2F5 MAb and the CD4-IgG2 molecule also neutralized strains from outside the B clade, with the same breadth and potency that they showed against B-clade strains. The other two MAbs were able to neutralize a significant proportion of strains from outside the B clade, although there was a reduction in their efficacy compared with their activity against B-clade isolates. Neutralization of isolates by 2F5 correlated with their possession of the LDKW motif in a segment of gp41 near the membrane-spanning domain. The other two MAbs and CD4-IgG2 recognize discontinuous binding sites on gp120, and so no comparison between genetic sequence and virus neutralization was possible. Our data show that a vaccine based on the induction of humoral immunity that is broadly active across the genetic clades is not impossible if immunogens that express the epitopes for MAbs such as 2F5, 2G12, and IgG1b12 in immunogenic configurations can be created. Furthermore, if the three MAbs and CD4-IgG2 produce clinical benefit in immunotherapeutic trials in the United States or Europe, they may also do so elsewhere in the world.
Trends in COPD Morbidity and Mortality in the United States (1989)

This paper describes patterns and trends in COPD morbidity and mortality based on data from several data systems of the National Center for Health Statistics (NCHS), as follows: mortality data from the National Vital Statistics System; and morbidity data from the National Health Interview Survey, the National Hospital Discharge Survey, and the National Ambulatory Medical Care Survey. The paper focuses on the period 1979 to 1985 and the age groups 55 years old and over. old and over. Data are also presented separately for men and women. In addition to presenting rates for prevalence, hospital discharges, office visits, and deaths on a population basis, the paper also presents rates and analyses on a per case basis, using a "pseudo-natural history model." This paper also describes some of the methodological issues associated with analyzing comparative data from several NCHS data systems.
Deep Sequencing of Antiviral T-Cell Responses to HCMV and EBV in Humans Reveals a Stable Repertoire That Is Maintained for Many Years (2012)

CD8(+) T-cell responses against latent viruses can cover considerable portions of the CD8(+) T-cell compartment for many decades, yet their initiation and maintenance remains poorly characterized in humans. A key question is whether the clonal repertoire that is raised during the initial antiviral response can be maintained over these long periods. To investigate this we combined next-generation sequencing of the T-cell receptor repertoire with tetramer-sorting to identify, quantify and longitudinally follow virus-specific clones within the CD8(+) T-cell compartment. Using this approach we studied primary infections of human cytomegalovirus (hCMV) and Epstein Barr virus (EBV) in renal transplant recipients. For both viruses we found that nearly all virus-specific CD8(+) T-cell clones that appeared during the early phase of infection were maintained at high frequencies during the 5-year follow-up and hardly any new anti-viral clones appeared. Both in transplant recipients and in healthy carriers the clones specific for these latent viruses were highly dominant within the CD8(+) T-cell receptor Vβ repertoire. These findings suggest that the initial antiviral response in humans is maintained in a stable fashion without signs of contraction or changes of the clonal repertoire.
Mechanisms of impaired beta-adrenergic receptor signaling in G(alphaq)-mediated cardiac hypertrophy and ventricular dysfunction. (2000)

Targeted cardiac overexpression of the alpha-subunit of the heterotrimeric G protein G(q) in transgenic mice evokes hypertrophy and depressed stimulation of cardiac inotropy and chronotropy by beta-adrenergic receptor (betaAR) agonists in vivo, which is a hallmark of many forms of experimental and human heart failure. The molecular basis of this betaAR dysfunction was explored in transgenic mice overexpressing G(alphaq) approximately 5-fold over background. Isoproterenol-stimulated adenylyl cyclase activities in myocardial membranes were significantly depressed in G(alphaq) mice compared with nontransgenic controls (19.7 +/- 2.6 versus 43.7 +/- 5. 6 pmol/min/mg) without a decrease in betaAR expression levels. Functional coupling of both betaAR subtypes was impaired. Similarly, in whole-cell patch-clamp studies, betaAR stimulation of L-type Ca(2+) channel currents was depressed approximately 75% in the G(alphaq) mice. Cardiac betaAR from these mice showed decreased formation of the active high-affinity conformation (R(H) = 29% versus 62% for nontransgenic littermates), confirming a receptor-G(s)-coupling defect. Of the three candidate kinases that might impose this uncoupling by receptor phosphorylation (protein kinase A, betaAR kinase, protein kinase C), only protein kinase C activity was elevated in G(alphaq) mouse hearts. Type V adenylyl cyclase was decreased approximately 45% in these mice, consistent with decreased basal, NaF, and forskolin-stimulated enzyme activities. Although cellular G(s) levels were unaltered, G(i2) and G(i3) were increased in G(alphaq) mice. Pertussis toxin treatment of isolated G(alphaq) myocytes resulted in an improvement in betaAR, but not that of forskolin or NaF, stimulation of adenylyl cyclase. Thus three distinct mechanisms contribute to impaired betaAR function by in vivo G(q) signaling cross-talk in myocytes. Because many elements of hypertrophy and/or failure in cellular and animal models can be initiated by increased G(alphaq) signaling, the current work may be broadly applicable to interfaces whereby modification of heart failure might be considered.
A Review of CO2 Capture by Absorption and Adsorption (2012)

Global warming resulting from the emission of greenhouse gases, especially CO2, has become a widespread concern in the recent years. Though various CO2 capture technologies have been proposed, chemical absorption and adsorption are currently believed to be the most suitable ones for post-combustion power plants. The operation of the chemical absorption process is reviewed in this work, together with the use of absorbents, such as the ionic liquid, alkanolamines and their blended aqueous solutions. The major concerns for this technology, including CO2 capture efficiency, absorption rate, energy required in regeneration, and volume of absorber, are addressed. For adsorption, in addition to physical adsorbents, various mesoporous solid adsorbents impregnated with polyamines and grafted with aminosilanes are reviewed in this work. The major concerns for selection of adsorbent, including cost, adsorption rate, CO2 adsorption capacity, and thermal stability, are compared and discussed. More effective and less energy-consuming regeneration techniques for CO2-loaded adsorbents are also proposed. Future works for both absorption and adsorption are suggested.
The Development of Iron Chelators for Clinical Use (1993)

Physiology and Pathophysiology of Iron Metabolism: Implications for Iron Chelation Therapy in Iron Overload (P. Ponka). Iron, Oxidative Damage and Chelating Agents (B. Halliwell). Iron Chelation Therapy in the Treatment of Iron Overload (M.J. Pippard). Potential Clinical Applications of Iron Chelating Therapy (C. Hershko, G. Link, M. Tzahor, and A. Pinson). Iron Withholding: The Natural Defense System and Its Modification by Pharmacologic Agents (E.D. Weinberg). Iron Chelation as a Chemotherapeutic Strategy in the Treatment of Malaria (V.R. Gordeuk, G.M. Brittenham, and P.E. Thuma). Towards Better Chelation Therapy: Current Concepts and Research Strategy (H.P. Schnebli, I. Hassan, K.O. Hamilton, S. Lynch, Y. Jin, H.P. Nick, H.H. Peter, U. Junker Walker, R. Ziel, S.C. Khanna, R. Dean, and R.J. Bergeron). Siderophores as Biological Deferration Agents and the Regulation of their Synthesis by Iron (J.B. Neilands). Biochemical and Genetic Studies on Lysine: N6-hydroxylase Involved in Aerobactin Synthesis (A.M. Thariath, M.A. Valvano, and T. Viswanatha). The Ferrichrome Biosynthetic Pathway of Ustilago maydis Reveals Similarities to the Aerobactin Pathway of Escherichia Coli and to Globin Gene Regulation in Erythroid Cells (S.A. Leong, B. Mei, C. Voisard, A. Budde, J. McEvoy, J. Wang, P. Xu, and B. Saville). Ability of Catecholate Derivatives to Promote Growth of Gram-Negative Bacteria (R. Reissbrodt and W. Rabsch). Synthesis and Biological Activity of Hydroxamate-Based Iron Chelators (R.J. Bergeron and J.S. McManis). Siderophore-Mediated Drug Delivery: The Design, Synthesis, and Study of Siderophore-Antibiotic and Antifungal Conjugates (M.J. Miller and F. Malouin). Siderophore-Based Hydroxypyridonate Sequestering Agents (K.N. Raymond and J. Xu). Ligand Design of Chelating Agents Effective in the Coordination of Fe(III) and for the Removal of Iron in Cases of Iron Overload (A.E. Martell, R.J. Motekaitis, Y. Sun, and E.T. Clarke). The Design of Therapeutically Useful Iron Chelators (R.C. Hider, J.B. Porter, and S. Singh). A Comparative Evaluation of Iron Chelators in a Primate Model (H.H. Peter, R.J. Bergeron, R.R. Streiff, and J. Wiegand). Preliminary Results from a Phase I Clinical Trial of HBED (R.W. Grady, A.D. Salbe, M.W. Hilgartner, and P.J. Giardina).
Construction, expression and characterization of chimaeric toxins containing the ribonucleolytic toxin restrictocin: intracellular mechanism of action (1997)

Restrictocin is a ribonucleolytic toxin produced by the fungus Aspergillus restrictus. Two chimaeric toxins containing restrictocin directed at the human transferrin receptor have been constructed. Anti-TFR(scFv)-restrictocin is encoded by a gene produced by fusing the DNA encoding a single-chain antigen-combining region (scFv) of a monoclonal antibody, directed at the human transferrin receptor, at the 5' end of that encoding restrictocin. The other chimaeric toxin, restrictocin-anti-TFR(scFv), is encoded by a gene fusion containing the DNA encoding the single-chain antigen-combining region of antibody to human transferrin receptor at the 3' end of the DNA encoding restrictocin. These gene fusions were expressed in Escherichia coli, and fusion proteins purified from the inclusion bodies by simple chromatography techniques to near-homogeneity. The two chimaeric toxins were found to be equally active in inhibiting protein synthesis in a cell-free in vitro translation assay system. The chimaeric toxins were selectively toxic to the target cells in culture with potent cytotoxic activities. However, restrictocin-anti-TFR(scFv) was more active than anti-TFR(scFv)-restrictocin on all cell lines studied. By using protease and metabolic inhibitors, it can be shown that, to manifest their cytotoxic activity, the restrictocin-containing chimaeric toxins need to be proteolytically processed intracellularly and the free toxin or a fragment thereof thus generated is translocated to the target via a route involving the Golgi apparatus.
A phase 3, multicenter, double-blind, placebo-controlled trial of lenvatinib (E7080) in patients with 131I-refractory differentiated thyroid cancer (SELECT). (2014)

LBA6008 Background: Lenvatinib (LEN) is an oral tyrosine kinase inhibitor of the VEGFR1-3, FGFR1-4, PDGFRβ, RET, and KIT signaling networks. Based on efficacy results of the phase 2 study of patients (pts) with 131 I-refractory differentiated thyroid cancer (RR-DTC), this phase 3 Study of (E7080) Lenvatinib in Differentiated Cancer of the Thyroid (SELECT) was developed. Methods: This randomized, double-blind, placebo (PBO)-controlled study enrolled pts with RR-DTC with documented disease progression within 13 months (mo). Pts were stratified by age (≤65, &gt;65 years), region and ≤1 prior VEGFR-targeted therapies and randomized 2:1 to LEN or PBO (24mg/d, 28-d cycle). Upon progression, pts receiving PBO could crossover to open-label LEN. The primary endpoint was PFS assessed by Independent Radiologic Review; secondary endpoints included overall response rate (ORR; complete response [CR] + PR), overall survival (OS) and safety. Results: 392 pts (63.0 years median age; 51.0% male) were randomized. Pts on LEN had a significantly prolonged PFS vs PBO (hazard ratio 0.21, 95% confidence interval [CI] 0.14–0.31; P &lt;.0001); median PFS was LEN: 18.3 mo (95% CI 15.1–not evaluable), PBO: 3.6 mo (95% CI 2.2–3.7). A LEN PFS benefit was observed in all predefined subgroups; median LEN PFS for pts with prior vs no prior VEGF-therapy was 15.1 mo (n=66) and 18.7 mo (n=195), respectively. Rates (n) of CRs were LEN: 1.5% (4), PBO: 0; PRs were LEN: 63.2% (165), PBO: 1.5% (2).Median exposure duration was LEN: 13.8 mo, PBO: 3.9 mo; median time to LEN response was 2.0 mo. Median OS has not been reached; deaths per arm were LEN: 71 (27.2%), PBO: 47 (35.9%). The 5 most common LEN treatment-related adverse events (TRAEs; any grade) were hypertension (68%), diarrhea (59%), appetite decreased (50%), weight loss (46%), nausea (41%). LEN grade ≥3 TRAEs (≥5%) were hypertension (42%), proteinuria (10%), weight loss (10%), diarrhea (8%), appetite decreased (5%). The dose was reduced in 78.5% of pts and discontinued due to adverse events (AEs) in 14.2% of pts. Conclusions: LEN significantly improved PFS compared with PBO in pts with progressive RR-DTC. There were no unexpected toxicities and AEs were manageable. Clinical trial information: NCT01321554.
Layer 2 and 3 virtual private networks: taxonomy, technology, and standardization efforts (2004)

Virtual private network services are often classified by the OSI layer at which the VPN service provider's systems interchange VPN reachability information with customer sites. Layer 2 and 3 VPN services are currently being designed and deployed, even as the related standards are being developed. This article describes the wide range of emerging L2 and L3 VPN architectures and technical solutions or approaches, and discusses the status of standards work. Some specific L2VPN and L3VPN technologies described here include virtual private LAN service, transparent LAN service, BGP/MPLS-based VPNs (RFC 2547bis), virtual router, and IPSec VPN approaches. We discuss recent and continuing standards efforts in the IETF 12vpn and 13vpn working groups, and related work in the pseudo-wire emulation edge-to-edge working group, as well as in some other standards fora, and describe some mechanisms that provide membership, reachability, topology, security, and management functions.
Interleukin‐10‐induced CD8 cell proliferation (1999)

Interleukin (IL)-10, a product of T helper 2 (Th2) lymphocytes, has been shown to be an important regulator of lymphoid and myeloid cells, inhibiting mitogen, peptide and alloantigen-induced T-cell proliferation and IL-2 production. The microenvironment at the time of cell activation, notably the presence or absence of cytokines such as IL-10, interferon-gamma (IFN-gamma) and IL-2, is believed to determine the lineage and magnitude of cell-mediated responses. In this study, we show that recombinant human IL-10 (rhIL-10) exerts a dose-dependent inhibitory effect on human peripheral blood mononuclear cells stimulated in vitro, when these cells have not previously been exposed to rhIL-10. Furthermore, incubation of these cells with high doses of rhIL-10, either before or at the time of activation, results in inhibition which is followed several days later by the emergence of a population of CD8 positive cells. This rhIL-10-responsive CD8, positive cell population still emerges even when the cells are washed following incubation with rhIL-10 prior to cell activation. Using purified CD8 populations this was shown to be a direct action of rhIL-10 on CD8 cells and not via CD4 positive cells and monocytes. This finding was only observed when cells were activated with a cross-linking anti-CD3 antibody and not when activated with phorbol-12-mystrate-13-acetate (PMA) and calcium ionophore (CaIon), suggesting that the effect is mediated through cell-surface receptors. Analysis of CD8 positive clones reveal production of Tc2 patterns of cytokines and reduced cell cytotoxicity to allogeneic, natural killer and lymphokine activated cell targets.
Advanced Metering for Phase Identification, Transformer Identification, and Secondary Modeling (2012)

Advanced metering infrastructure (AMI) offers utilities new ways to model and analyze distribution circuits. Results from two circuits introduce a new method to identify phasing of transformers and single-phase taps using voltage and kilowatt-hour measurements from AMI. In addition to phase identification, we show how to use the same approach to create or check meter-to-transformer mappings. These algorithms are based on linear regression and basic voltage drop relationships. With this approach, secondary connectivity and impedance models can be auto generated. In addition, detection of unmetered load appears possible. Also demonstrated is use of AMI to estimate primary-side voltage profiles.
Status of current research on endometriosis. (1998)

This paper reviews international issues in the supply of tobacco and tobacco products, including trade liberalization and globalization. The paper begins with a brief discussion of the theoretical foundations for trade and trade restrictions. This is followed by a description of the treatment of tobacco and tobacco products in recent multi-lateral, regional and bilateral trade agreements, as well as a short discussion of the recent globalization of the tobacco industry. Included in this description is a review of the empirical evidence on the impact of trade liberalization on tobacco use. The implications of two recently proposed international agreements--the Multilateral Agreement on Investments and the Framework Convention for Tobacco Control--are then discussed. The paper concludes by summarizing the theoretical and empirical evidence that shows clearly that trade liberalization has significantly increased tobacco use, particularly in low and middle-income countries, and follows this with a discussion of the lessons learned from tobacco for controlling alcohol supply.
Production of paramylon, a β‐1,3‐glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium (2009)

Abstract Euglena gracilis was cultivated on a synthetic medium of well‐defined components. Biomass and paramylon (β‐1,3‐glucan) concentrations were the most important variables monitored. Mass production in the bioreactor was carried out following studies of operating conditions in shaken flasks. E. gracilis grew best at about 30°C and at a low pH of 3. A pH control was not necessary, although the pH increased considerably at the end of the cultivation processes. Aeration could be performed at low stirrer frequency. Biomass concentrations of about 13–14 g/L were obtained with paramylon mass fractions of 50–60%, by starting with a synthetic medium containing 15 g/L of glucose as the main carbon source.
The Mammalian Immediate-early TIS21 Protein and the Leukemia-associated BTG1 Protein Interact with a Protein-arginine -Methyltransferase (1996)

The TIS21 immediate-early gene and leukemia-associated BTG1 gene encode proteins with similar sequences. Two-hybrid analysis identified a protein that interacts with TIS21 and BTG1. Sequence motifs associated with S-adenosyl-L-methionine binding suggested this protein might have methyltransferase activity. A glutathione S-transferase (GST) fusion of the putative methyltransferase modifies arginine residues, in appropriate protein substrates, to form NG-monomethyl and NG,NG-dimethylarginine (asymmetric). We term the protein- arginine N-methyltransferase (EC 2.1.1.23) gene "PRMT1, " for protein-arginine methyltransferase 1. GST-TIS21 and GST-BTG1 fusion proteins qualitatively and quantitatively modulate endogenous PRMT1 activity, using control and hypomethylated RAT1 cell extracts as methyl-accepting substrates. PRMT1 message appears ubiquitous, and is constitutive in mitogen-stimulated cells. Modulation of PRMT1 activity by transiently expressed regulatory subunits may be an additional mode of signal transduction following ligand stimulation.
Compact and Efficient Bipolar Coupler for Wireless Power Chargers: Design and Analysis (2015)

Compactness and efficiency are the two basic considerations of the wireless battery chargers for electric vehicles (EVs) and plug-in hybrid EVs. The double-sided LCC compensation topology for wireless power transfer (WPT) has been proved to be one of the efficient solutions lately. However, with the increase of the numbers of compensation components, the volume of the system may become larger, which makes it less attractive. To improve the compactness, a bipolar coupler structure with a compensation-integrated feature is proposed. The inductors of the LCC compensation networks are designed as planar-type and attached to the power-transferring main coils. Extra space and magnetic cores for the compensated inductors outside of the coupler are saved. The cost is that extra couplings between the compensated coils (inductors) and the main coils are induced. To validate the feasibility, the proposed coupler is modeled and investigated by 3-D finite-element analysis tool first. The positioning of the compensated coils, the range of the extra couplings, and the tolerance to misalignment are studied. This is followed by the circuit modeling and characteristic analysis of the proposed WPT topology based on the fundamental harmonic approximation. At last, a 600 mm × 600 mm with a nominal 150-mm-gap wireless charger prototype, operated at a resonant frequency of 95 kHz and a rated power of 5.6 kW has been built and tested. A peak efficiency of 95.36% from a dc power source to the battery load is achieved at rated operation condition.