gebhart/patrepeval huggingface dataset:
Highly Aminated Mesoporous Silica Nanoparticles with Cubic Pore Structure (2010)

Mesoporous silica with cubic symmetry has attracted interest from researchers for some time. Here, we present the room temperature synthesis of mesoporous silica nanoparticles possessing cubic Pm3n symmetry with very high molar ratios (>50%) of 3-aminopropyl triethoxysilane. The synthesis is robust allowing, for example, co-condensation of organic dyes without loss of structure. By means of pore expander molecules, the pore size can be enlarged from 2.7 to 5 nm, while particle size decreases. Adding pore expander and co-condensing fluorescent dyes in the same synthesis reduces average particle size further down to 100 nm. After PEGylation, such fluorescent aminated mesoporous silica nanoparticles are spontaneously taken up by cells as demonstrated by fluorescence microscopy.
Potential usefulness of fish oil in the primary prevention of acute coronary syndrome (2009)

Acute coronary syndrome (ACS) is a major health problem and leads to a large number of hospitalizations annually in Europe.1 ACS refers to a group of clinical conditions caused by myocardial ischaemia, including unstable angina pectoris, non-ST-segment elevation myocardial infarction (NSTEMI), ST-segment elevation myocardial infarction (STEMI), and sudden cardiac death. ACS results from a common underlying pathophysiological mechanism, i.e. plaques vulnerable to rupture or erosion, with different degrees of superimposed thrombosis and distal embolization. Vulnerable plaques that are likely to rupture or erode have evidence of inflammation, together with thin fibrous caps and large lipid cores. The platelet-rich thrombus, developed in association with pro-coagulant-vulnerable blood, can release vasoconstrictor substances such as serotonin and thromboxane A2 that may induce vasoconstriction at the site of plaque rupture or in the … 

*Corresponding author. Tel: +81 22 717 7153, Fax: +81 22 717 7156, Email: shimo{at}cardio.med.tohoku.ac.jp
The Prognostic Value of Some Clinical and Histological Parameters in Membranoproliferative Glomerulonephritis (MPGN) (1977)

112 cases of MPGN, whose diagnosis was made on light microscopy, were reviewed. Histological examination showed 66 cases of 'classical' MPGN, 33 of MPGN with a lobular pattern and 13 of MPGN with epithelial crescents. In 11 patients dense intramembranous deposits were observed. On immunofluorescence (95 cases) 62 patients showed deposits of C3 together with immunoglobulins, 20 had a predominant deposition of C3 and in 13 C3 alone was present. At the moment of biopsy 57 patients had nephrotic syndrome, 43 hypertension, 43 impaired renal function and 65 hypocomplementaemia. In 23 cases, one or more episodes of macroscopic haematuria occurred. The actuarial survival was 70% after 10 years and 50% after 20 years from onset. At last observation 25 patients were dead or on haemodialysis, 22 had impaired renal function, 62 had normal renal function and 2 were in complete remission. The histological variety with epithelial crescents had a significantly worse outcome. The presence of dense deposits or of any specific immunofluorescence pattern had no prognostic significance. Nephrotic syndrome, renal function impairment and hypertension indicated a poor prognosis: however, macroscopic hamaturia or hypocomplementaemia did not influence the outcome.
Discrepant results in the interpretation of HIV‐1 drug‐resistance genotypic data among widely used algorithms (2003)

This work demonstrates the great level of discordance in the interpretation of genotyping results among algorithms, clearly showing the necessity for clinical validation. Moreover, these results suggest that a joint effort from the scientific community as well as national and international HIV societies is needed to achieve a consensus for the interpretation of genotypic data.
Artificial Lotus Leaf by Nanocasting (2005)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTArtificial Lotus Leaf by NanocastingManhui Sun, Chunxiong Luo, Luping Xu, Hang Ji, Qi Ouyang, Dapeng Yu, and Yong ChenView Author Information Center for Microfluidics and Nanotechnology, Peking University, 100871 Beijing, PR China, School of Physics, Peking University, 100871 Beijing, PR China, and Ecole Normale Suéprieure, 24 rue Lhomond, 75231 Paris, France Cite this: Langmuir 2005, 21, 19, 8978–8981Publication Date (Web):August 16, 2005Publication History Received4 February 2005Revised22 July 2005Published online16 August 2005Published inissue 1 September 2005https://pubs.acs.org/doi/10.1021/la050316qhttps://doi.org/10.1021/la050316qbrief-reportACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views7878Altmetric-Citations602LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Contact angle,Genetics,Hydrophobicity,Hysteresis,Liquids Get e-Alerts
Augmentation of Thyroid Hormone Receptor-Mediated Transcription by Ca2+/Calmodulin-Dependent Protein Kinase Type IV (2000)

Thyroid hormone receptor (TR), a ligand-mediated transcription factor, binds to a DNA sequence known as a thyroid-hormone response element (TRE) to activate or repress transcription of target genes. Recently, studies have shown that Ca2+/calmodulin-dependent protein kinases (CaMKs) may be involved in regulating gene transcription via phosphorylation of specific transcription factors, including RORα, a retinoic acid-related orphan nuclear hormone receptor. In this light, we examined the effect of CaMK type IV (CaMKIV) and RORα, which also shown to influence thyroid hormone action, on TR-mediated transcription using a transient transfection assay. Expression vectors containing TR, vitamin D receptor (VDR), and estrogen receptor (ER) were cotransfected in CV-1 cells with RORα and/or constitutively active CaMKIV and thymidine kinase promotor-luciferase reporter vector containing their cognate response elements. When CaMKIV or RORα was co-transfected with TR, the T3-induced transcription was significantly augmented compared to that induced by TR alone. When both were co-transfected with TR, T3-induced transcription was augmented additively. In contrast, the augmentation by CaMKIV or ROR on ligand-induced transcription was not detected with VDR and ER. Hence, these results indicate that the augmentation mediated by CaMKIV and RORα is specific for TR-mediated transcription on TRE. Our results suggest that CaMKIV, as well as RORα, play important roles in TR-mediated transcription on TREs.
Expression of hepatitis B virus surface antigen in adult rat liver. Co-introduction of DNA and nuclear protein by a simplified liposome method (1991)

We established a simple and efficient method for gene transfer in vitro (to cultured cells) and in vivo (to an adult organ) using liposomes.Plasmid DNA and proteins were efficiently co-encapsulated in liposomes by agitation and sonication, and were co-introduced into cells by hemagglutinating virus of Japan (HVJ)-mediated membrane fusion.Introduction of the Escherichia coli &galactosidase gene with non-histone chromosomal protein high mobility group 1 (HMG1) into LLCMKz cells resulted in about 3 times higher 8-galactosidase activity than that on introduction of the gene alone.Two days after injection of HVJ-liposomes containing the &galactosidase gene and HMGl under the perisplanchnic membrane of adult rat liver, hepatic cells near the injection site were found by 5-bromo-4chloro-3-indolyl fl-D-galactoside staining to have Bgalactosidase activity.After similar injection of HVJliposomes containing the hepatitis B virus surface antigen (HBsAg) gene and HMG1, HBsAg was detected in the serum for 9 days with a maximum of 25-45 ngl ml on day 2 after the injection.Methods for introducing exogenous genes into various types of cells in vitro and in uiuo have been developed to study the biological functions of macromolecules, to establish animal models of human diseases, and for postnatal gene therapy.Many methods for introducing macromolecules into cultured cells (in vitro) have been reported (1, 2).Methods for gene transfer by liposomes and HVJ' have been developed in our laboratory (3, 4).Frequency of gene transfer by the liposome method into adhesive cultured cells is comparable to that by calcium phosphate precipitation, and this method
The role of CD146 (Mel-CAM) in biology and pathology (1999)

CD146, also known as Mel-CAM, MUC18, A32 antigen, and S-Endo-1, is a membrane glycoprotein which functions as a Ca(2+)-independent cell adhesion molecule involved in heterophilic cell-cell interactions. Based on homology of the nucleotide sequence, CD146 is classified as a member of the immunoglobulin gene superfamily, since it contains the characteristic V-V-C2-C2-C2 immunoglobulin-like domain structure. Using immunohistochemistry with CD146-specific antibodies, CD146 expression has been demonstrated in a relatively limited spectrum of normal human tissues and malignant neoplasms. The lineage-specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. Although the biological role of CD146 in normal tissue and malignant tumours remains unclear, CD146 has been suggested to play an important role in tumour progression, implantation and placentation. CD146 expression can promote tumour progression in human melanoma, possibly through enhanced interaction between melanoma cells and endothelial cells. In contrast, CD146 may act as a tumour suppressor in breast carcinoma. CD146 expression is frequently lost in breast carcinomas and overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and the formation of smaller tumours in nude mice. During implantation and placentation, CD146 expressed by the intermediate trophoblast in the placental site binds to its putative receptor in uterine smooth muscle cells and limits trophoblastic invasion in the myometrium. In conclusion, CD146 is a recently identified novel cell adhesion molecule and its biological functions and role as a diagnostic marker in pathology are now being recognized. Identification of the receptor for CD146 and the development of experimental models that can account for the complex interactions between CD146-expressing cells and their microenvironment are needed to investigate further the functions of this molecule in biology and in pathological states.
Recombinant C1 inhibitor in brain ischemic injury (2009)

rhC1-INH showed a surprisingly wider time window of efficacy compared with the corresponding plasmatic protein. We propose that the superiority of rhC1-INH is due to its selective binding to MBL, which emerged as a novel target for stroke treatment.
The DSD Schema Language and its Applications (2000)

XML (eXtensible Markup Language), a linear syntax for trees, has gathered a remarkable amount of interest in industry. The acceptance of XML opens new venues for the application of formal methods such as specification of abstract syntax tree sets and tree transformations. A user domain may be specified as a set of trees. For example, XHTML is a user domain corresponding to the set of XML documents that make sense as&lt;br /&gt;HTML. A notation for defining such a set of XML trees is called a schema language. We believe that a useful schema notation must identify most of the syntactic&lt;br /&gt;requirements that the documents in the user domain follow; allow efficient parsing; be readable to the user; allow a declarative default notation `a la CSS; and be&lt;br /&gt;modular and extensible to support evolving classes of XML documents. In the present paper, we give a tutorial introduction to the DSD (Document Structure Description) notation as our bid on how to meet these requirements. The&lt;br /&gt;DSD notation was inspired by industrial needs, and we show how DSDs help manage aspects of complex XML software through a case study about interactive voice&lt;br /&gt;response systems (automated telephone answering systems, where input is through the telephone keypad or speech recognition). The expressiveness of DSDs goes beyond the DTD schema concept that is already&lt;br /&gt;part of XML. We advocate the use of nonterminals in a top-down manner, coupled with boolean logic and regular expressions to describe how constraints on tree nodes depend on their context. We also support a general, declarative mechanism for inserting default elements and attributes that is reminiscent of Cascading&lt;br /&gt;Style Sheets (CSS), a way of manipulating formatting instructions in HTML that is built into all modern browsers. Finally, we include a simple technique for evolving DSDs through selective redefinitions. DSDs are in many ways much more expressive than XML Schema (the schema language proposed by the W3C), but their syntactic and semantic definition in English is only 1/8th the size. Also, the DSD notation is self-describable: the syntax of legal DSD documents and all static semantic requirements can be captured in a DSD document, called the meta-DSD.
The Two-Component System1 (1998)

Unicellular microorganisms experience "life on the edge," as they have little ability to change their environment and face fierce competition for limited resources. They must therefore respond to a barrage of environmental cues in a rapid and accurate manner. Among multicellular organisms, plants in particular cannot escape their environment and so must be masters at adapting and coordinating cellular events to accommodate prevailing conditions. The penalty for losing touch with reality is often death. The study of sensory-response systems has defined the basics of how many organisms detect and respond with exquisite sensitivity to changes in their chemical or physical environments. Such studies have recently focused on events that occur at the cellular and molecular levels, elucidating the mechanisms of detecting extracellular signals and transducing such signals into the appropriate intracellular events. In a large number of cases, these signaling pathways involve phosphorylation of key effector proteins by protein kinases. In bacteria numerous sensory-response circuits operate by making use of a phosphorylation control mechanism referred to as the "two-component system"
A Study of the Formation of Single- and Double-Walled Carbon Nanotubes by a CVD Method (2001)

International audience
Localization of mitochondria in living cells with rhodamine 123. (1980)

The laser dye rhodamine 123 is shown to be a specific probe for the localization of mitochondria in living cells. By virtue of its selectivity for mitochondria and its fluorescent properties, the detectability of mitochondria stained with rhodamine 123 is significantly improved over that provided by conventional light microscopic techniques. With the use of rhodamine 123, it is possible to detect alterations in mitochondrial distribution following transformation by Rous sarcoma virus and changes in the shape and organization of mitochondria induced by colchicine treatment.
Structural and functional analysis of NF-kappa B. Determinants of DNA binding specificity and protein interaction. (1994)

The NF-kappa B transcription factors display a high degree of sequence conservation in a domain initially described in the rel oncogene. Two family members, NF-kappa B1 and NF-kappa B2, have distinct DNA binding properties and functionally distinct effects on different enhancers. NF-kappa B1, for example, binds to the kappa B site from the human immunodeficiency virus (HIV) with approximately 15-fold higher affinity than NF-kappa B2. In this study, we have defined regions within the Rel domain which determine DNA binding specificity and interaction with other proteins. We find that the COOH-terminal putative Rel dimerization domain of NF-kappa B1 is required for preferential binding to the HIV kappa B site. In contrast, preferential stimulation of the HIV enhancer by NF-kappa B2 with RelA(p65) is determined by both the NH2- and COOH-terminal Rel domains of NF-kappa B2. These two regions of NF-kappa B2 also mediate preferential synergy with Bcl3. These data suggest that a specific subdomain of the Rel conserved region has evolved to control the fine specificity of DNA binding, and two distinct subregions within the Rel domain determine the specificity of interaction with other transcription factors. These specific Rel-conserved domains therefore determine the specificity of NF-kappa B interactions and contribute to selective gene activation.
Supercontinuum generation by femtosecond single and dual wavelength pumping in photonic crystal fibers with two zero dispersion wavelengths (2005)

We investigate supercontinuum generation in photonic crystal fibers under femtosecond single and dual wavelength pumping experimentally and by numerical simulations. Details about the expansion of the blue but also the red side of the continuum due to cross-phase modulation (XPM) and transfer of energy to dispersive waves are revealed and experimentally confirmed. Additionally, simple guidelines are given to predicte the maximum bandwidth of supercontinuum generation only by the use of the dispersion curve of the fiber.
[Study on novel morphine antagonists in the animal experiment (author's transl)]. (1976)

The morphine-antagonistic properties of 5 novel compounds, (--)N-(3-Furylmethyl)-nordesomorphine-hydrochloride-monohydrate (Wa 494-Cl), (--)N-(3-Furylmethyl)-3-hydroxy-morphinan-methansulfonate (Mr 1257 MS), (--)2-(3-Furylmethyl)-2'-hydroxy-5,9alpha-dimethyl-6,7-benzomorphan-methansulfonate (Mr 1452 MS), (+/-)-2-(3-Furylmethyl)-2'-hydroxy-5,9alpha-diethyl-6,7-benzomorphan-hydrochloride (Mr 1302 MS), (--)-N-(3-Furylmethyl)-noroxymorphon-methanesulfonate (Mr 1767 MS), have been examined with different methods on mice and cats. Their properties have been compared with those of naloxone, cyclazocine and nalorphine. The new test compounds showed a profile of action like naloxone.
Dynamics of shock waves and cavitation bubbles generated by picosecond laser pulses in corneal tissue and water (1994)

Time-resolved flash photography was used to investigate the dynamics of shock waves and cavitation bubbles generated by picosecond optical breakdown in bovine corneal tissue and water. A picosecond Nd:YLF laser was employed. A rapid decay of the shock waves was observed in both materials, with similar temporal characteristics, indicating that water serves as a good model for shock wave studies. In contrast, differences in the cavitation bubble dynamics were found between cornea and water, which are related to differences in the mechanical and thermal properties of the two media, suggesting that water should not be used to model cavitation dynamics in cornea. The experimental results also suggest that the efficiency of intrastromal ablation may be increased by using short pulses and moderate pulse energies in order to avoid the creation of large cavitation bubbles. The experiment indicates that the optimum laser repetition rate for intrastromal ablation is between 1 and 5 kHz.
MiR‐29 mediates TGFβ1‐induced extracellular matrix synthesis through activation of PI3K‐AKT pathway in human lung fibroblasts (2012)

TGFβ1 is very important in the synthesis and degradation of extracellular matrix, and also in the mediation of human lung fibroblasts proliferation, and miR-29 plays an important role in this process. To explore the interactions of miR-29 family members and TGFβ1, the effects of transforming growth factor TGFβ1 on the expression of miR-29 and whether miR-29 is involved in pro-survival signaling pathways mediated by TGFβ1 were examined in human lung fibroblasts. Treatment of the human embryonic lung fibroblast cell line IMR90 with TGFβ1 caused a decrease in expression of miR-29a/b/c by real-time PCR analysis. TGFβ1 stimulation increased cell proliferation, colony formation and up-regulated expression of COL1A1; transfecting with miR-29a/b/c mimics reverse TGFβ1-induced phenotype changes in IMR90 cells. Western blot analyses showed that TGFβ1 treatment unchanged total protein expression levels of PI3K or AKT, but the expression levels of p-PI3K, p-AKT, and COL1A1 were increased; and miR-19a/b/c mimics interfering blocked phosphorylation of PI3K or AKT and decreased expression of COL1A1 after TGFβ1 treatment. The results indicate that TGFβ1 beta uses the PI3k-Akt pathway in these embryonic fibroblasts and miR29 blocks this activation pathway. It indicates a novel biological function of the PI3K-Akt pathway in IMR90. Elevated expression of miR-29 may play an important role in the pathogenesis of diseases related to fibrogenic reactions in human lung fibroblasts.
β‐Lactam Antibiotics and Gastrointestinal Colonization with Vancomycin‐Resistant Enterococci (2004)

We studied the effect of different subcutaneously administered beta-lactam antibiotics on the establishment of gastrointestinal colonization by vancomycin-resistant Enterococcus faecium C68 in a mouse model. Aztreonam, cefazolin, cefepime, and, to a lesser extent, ceftazidime, which neither have significant antienterococcal activity nor are secreted into human bile at high concentrations, did not promote significant vancomycin-resistant enterococci (VRE) colonization. Piperacillin-tazobactam, which has antienterococcal activity and is secreted in human bile at high concentrations, inhibited colonization after limited exposure to the inoculum but was associated with progressively increased VRE colony counts in stool samples after repeated exposure to the VRE inoculum. Ceftriaxone and cefotetan, which lack antienterococcal activity but are secreted into human bile at high concentrations, were associated with rapid and high-level colonization. These data suggest that the risk of VRE colonization varies during exposure to different beta-lactam antimicrobial agents and that the risk is related to biliary concentration and antienterococcal activity of the specific beta-lactam.
Enhancement of Peroxidase-Catalyzed Chemiluminescent Oxidation of Cyclic Diacyl Hydrazides by Arylboronic Acids (1994)

Journal Article Enhancement of Peroxidase-Catalyzed Chemiluminescent Oxidation of Cyclic Diacyl Hydrazides by Arylboronic Acids Get access Larry J Kricka, Larry J Kricka Dept. of Pathol. and Lab. Med., Univ. of Pennsylvania, 3400 Spruce St., Philadelphia, PA 19104 Search for other works by this author on: Oxford Academic Google Scholar Xiaoying Ji Xiaoying Ji Dept. of Pathol. and Lab. Med., Univ. of Pennsylvania, 3400 Spruce St., Philadelphia, PA 19104, Hosp. of the Univ. of Pennsylvania, Philadelphia, PA 19104 Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 40, Issue 9, 1 September 1994, Pages 1828–1830, https://doi.org/10.1093/clinchem/40.9.1828 Published: 01 September 1994