gebhart/patrepeval huggingface dataset:
Development and integration of generic components for a teachable vision-based mobile robot (1996)

This paper presents a mobile robotic system for human assistance in navigation-the robot navigates by receiving visual instructions from a human being and is able to replicate them autonomously. We describe three generic components defined as the HOST, the VISION, and the CONTROL components as well as their integration in our teachable mobile robot. These components are connected to each other via a transputer serial link, namely they are loosely coupled, they work in parallel and are asynchronous with each other. Each component is described with a peculiar feature of extensibility. Especially in the VISION component, there are two major features. The first one is a correlator which each vision board possesses. The correlator does block-matching between the template and the grabbed images in real-time. The other is the PIM library which manages the visual tasks over limited parallel visual resources of the mobile robot. These features of our design enable the system to be real-time and allow for efficient and extensible software development. In order to show the feasibility of our system design, we present a preliminary experiment of the route teaching on our mobile robot.
The pathogenesis and management of painful diabetic neuropathy: a review (2003)

Painful diabetic neuropathy has always been a challenging complication of diabetes mellitus. Emerging theories suggest that early dysaesthesia associated with painful neuropathy may act as a marker for the development of the 'at risk' foot, allowing preventative clinical strategies to be undertaken. The mechanisms of neuropathic pain are complex. The authors' intentions are to help members of the diabetes care team better understand and appreciate the diverse symptoms reported by patients. The various treatments available for painful neuropathy are discussed in detail. Robust comparative studies on such treatments are, however, unavailable and the authors have designed a logical approach to management based on best current evidence and their own clinical experience.
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins (1998)

The measurement of nitric oxide (NO) is important for direct examination of the regulatory roles of NO in various biological systems. Diaminofluoresceins (DAFs), new fluorescence indicators for NO, were applied to detect the release of NO from bovine aortic endothelial cells (ECs). DAFs react with NO to yield the corresponding green-fluorescent triazolofluoresceins, which provide the advantages of specificity, sensitivity and a simple protocol for the direct detection of NO. Using these DAFs, we could detect the generation of NO not only from inducible NO synthase expressed in macrophages, but also from constitutive NO synthase expressed in ECs.
Method for Improving Hydrocarbon/Water Ratios in Producing Wells (1986)

ABSTRACT Production of unwanted water has consistently burdened the industry since the costs of lifting, separating, and disposing of produced water make oil and gas production less profitable. Previous methods for reducing water production have involved the sequential pumping of two or more materials to form plugs within the formation to control water flow. These materials have been neither effective nor selective in-reducing only the flow of water. The recent introduction of new water control chemicals, which are designed to, reduce the flow of water while not effecting hydrocarbon flow have experienced limited success. One reason for the minimal success rate has been the inability of these polymers to withstand shear. In addition, poor candidate selection has created a high volume of unsuccessful treatments, causing operators to shy away from their use. A selective, one component system using a new amphoteric polymeric material (APM) has been developed which has the ability to reduce the effective permeability to water while retaining the formation permeability to oil or gas. In addition a candidate selection technique used to predict the likelihood of success of a water control treatment is presented.
Therapeutic efficacy of a high-affinity anticarcinoembryonic antigen monoclonal antibody (COL-1). (1993)

COL-1 is a murine IgG2a monoclonal antibody (MAb) with a high affinity (1.4 x 10(9) M-1) for carcinoembryonic antigen (CEA) and no detectable reactivity for CEA-related antigens, such as nonspecific cross-reacting antigen (NCA) and normal fecal antigen (NFA). 125I-labeled COL-1 IgG was shown to efficiently and specifically target the LS-174T human colon carcinoma xenograft in athymic mice. Dose titration studies in this same model with 131I-labeled COL-1 demonstrated reduction of tumor growth rate when 300 microCi of the immunoconjugate was used (0.005 > p > 0.001). Administration of higher levels as a single dose led to increased toxicity. Dose fractionation experiments with 131I-COL-1 demonstrated the ability to administer much higher levels of the immunoconjugate with little or no toxicity, which resulted in a greater therapeutic efficacy. For example, three fractions of 200 microCi of 131I-COL-1 given at weekly intervals (for a total of 600 microCi) resulted in the substantial reduction (p < 0.0005) of the growth of established tumors in 100% (7/7) of mice, and in no evidence of tumor growth in 71% (5/7) of mice, at the end of the 63-day observation period. These results thus demonstrate the potential therapeutic efficacy for radiolabeled COL-1 in clinical trials and demonstrate the principle of the advantage of dose fractionation protocols for this immunoconjugate.
Poly(glycoamidoamine) Brushes Formulated Nanomaterials for Systemic siRNA and mRNA Delivery in Vivo (2016)

Safe and effective delivery is required for siRNA and mRNA-based therapeutics to reach their potential. Here, we report on the development of poly(glycoamidoamine) brush nanoparticles as delivery vehicles for siRNA and mRNA. These polymers were capable of significant delivery of siRNA against FVII and mRNA-encoding erythropoietin (EPO) in mice. Importantly, these nanoparticles were well-tolerated at their effective dose based on analysis of tissue histology, systemic cytokine levels, and liver enzyme chemistry. The polymer brush nanoparticles reported here are promising for therapeutic applications.
Expression of Recombinant Human Thyrotropin Receptor in Myeloma Cells (1995)

Starting with a previously isolated cDNA for human thyrotropin receptor (TSHR), we established a transformed myeloma cell line, SP56, which expresses human TSHR on its cell surface. Binding analysis showed that SP56 bears 1.1 x 10(5) TSHR per cell with a Kd of 2.2 x 10(-10) M. Using the purified cellular membrane, we established a TSH binding inhibition immunoglobulin (TBII) assay for autoantibodies against TSHR. We compared it with the TBII assay utilizing porcine thyroid membranes expressing porcine TSHR, which has been widely used for TBII assay, by using 96 serum samples from patients with autoimmune thyroid disease and normal individuals. Our TBII assay was more sensitive than the one using porcine TSHR: of 38 sera of patients which were judged negative for autoantibodies to TSHR (TBII value below 10%) by the latter assay, 28 were positive (above 20%) in our assay. By using a perfusion culture system, we obtained as many as 3 x 10(10) SP56 cells, from which 3,450 mg protein of the membrane could be purified; this is sufficient for 15,000 assays. The results indicate that the membrane of the myeloma cell line SP56 is more suitable for use in the TBII assay than the porcine thyroid membrane, in terms of sensitivity to autoantibodies against TSHR in human sera.
Targeting the MDM2-p53 Interaction for Cancer Therapy (2008)

p53 is a powerful tumor suppressor and is an attractive cancer therapeutic target because it can be functionally activated to eradicate tumors. The gene encoding p53 protein is mutated or deleted in half of human cancers, which inactivates its tumor suppressor activity. In the remaining cancers with wild-type p53 status, its function is effectively inhibited through direct interaction with the human murine double minute 2 (MDM2) oncoprotein. Blocking the MDM2-p53 interaction to reactivate the p53 function is a promising cancer therapeutic strategy. This review will highlight the advances in the design and development of small-molecule inhibitors of the MDM2-p53 interaction as a cancer therapeutic approach.
ABCA1-mediated transport of cellular cholesterol and phospholipids to HDL apolipoproteins (2000)

Lipid-poor apolipoproteins remove cellular cholesterol and phospholipids by an active transport pathway controlled by an ATP binding cassette transporter called ABCA1 (formerly ABC1). Mutations in ABCA1 cause Tangier disease, a severe HDL deficiency syndrome characterized by a rapid turnover of plasma apolipoprotein A-I, accumulation of sterol in tissue macrophages, and prevalent atherosclerosis. This implies that lipidation of apolipoprotein A-I by the ABCA1 pathway is required for generating HDL particles and clearing sterol from macrophages. Thus, the ABCA1 pathway has become an important therapeutic target for mobilizing excess cholesterol from tissue macrophages and protecting against atherosclerosis.
The primary structure of human erythrocyte copper/zinc superoxide dismutase: cleavage with Staphylococcus aureus protease, determination of the N-terminal blocking group and location of the disulfide bond. (1982)

Results obtained after digestion of copper/zinc superoxide dismutase from human erythrocytes with S. aureus protease are described. In particular, peptides soluble in alkaline conditions proved essential for completing the determination of the primary structure of the enzyme; other peptides were important for establishing the amidation state of dicarboxylic amino acid residues and for confirming controversial sequences. The human enzyme is acetylated at the NH2 terminus and contains an intrasubunit disulfide bond connecting half-cystine residues 57 and 146.
Dealing with textureless regions and specular highlights - a progressive space carving scheme using a novel photo-consistency measure (2003)

We present two extensions to the space carving framework. The first is a progressive scheme to better reconstruct surfaces lacking sufficient textures. The second is a novel photo-consistency measure that is valid for both specular and diffuse surfaces, under unknown lighting conditions.
A Microfabricated Thermal Field-Flow Fractionation System (2002)

A microscale thermal field-flow fractionation (micro-TFFF) system has been designed, fabricated, and characterized. Motivation for miniaturization of TFFF systems was established by examining the geometrical scaling of the fundamental TFFF theory. Miniaturization of conventional macroscale TFFF systems was made possible through utilization of micromachining technologies. Fabrication of the micro-TFFF system was discussed in detail. The micro-TFFF system was characterized for plate height versus flow rate, single-component polystyrene retention, and multicomponent polystyrene separations. Retention, thermal diffusion coefficients, and maximum diameter-based selectivity values were extracted from separation data and found comparable with macroscale TFFF system results. Retention values ranged from 0.33 to 0.46. Thermal diffusion coefficients were between 3.0 x 10(-8) and 5.4 x 10(-8) cm2/s x K. The maximum diameter-based selectivity was 1.40.
Elevated Serum Levels of Interferon-Regulated Chemokines Are Biomarkers for Active Human Systemic Lupus Erythematosus (2006)

These data suggest that severely disrupted chemokine gradients may contribute to the systemic autoimmunity observed in human SLE. Furthermore, the levels of serum chemokines may serve as convenient biomarkers for disease activity in lupus.
The Corepressor Silencing Mediator for Retinoid and Thyroid Hormone Receptor Facilitates Cellular Recovery from DNA Double-Strand Breaks (2006)

Cells are frequently challenged by DNA double-strand breaks (DSB) that threaten their normal function and survival. In mammalian cells, the repair of DSBs is predominantly mediated by the DNA-dependent protein kinase (DNA-PK) complex. We unexpectedly found that the corepressor silencing mediator for retinoid and thyroid hormone receptor (SMRT) associates with the DNA-PK repair complex. The SMRT/histone deacetylase 3 complex is required for the transcriptional repressive property of the Ku70 subunit of the repair complex. Moreover, SMRT, but not the related Nuclear Receptor Corepressor, is required for cellular recovery from DNA DSBs induced by ionizing radiation or DNA damage-inducing drugs. Thus, the corepressor SMRT plays a novel and critical role in the cellular response to DSBs.
Translation of a synthetic two-cistron mRNA in Escherichia coli. (1986)

A synthetic two-cistron expression system was constructed for the high-level expression of eukaryotic genes in Escherichia coli. This system was designed to overcome translational inhibition of mRNAs containing eukaryotic sequences. The first cistron in this system is a 31-base A + T-rich synthetic sequence that provides for efficient translation initiation. The second cistron contains the protein coding sequence for the eukaryotic gene. Insertion of the first cistron between the 5' untranslated region of the mRNA and the protein coding region separates the two and thereby potentially minimizes the formation of local secondary structures that might prevent ribosomes from binding and initiating translation. The 31-base cistron contains three nonsense codons (TAA), one in each of the three translational reading frames, and an 8-base Shine-Dalgarno sequence that is complementary to the 3' end of the 16S rRNA. The effects of translation of the first cistron in all three reading frames on the expression of the second cistron was examined. The most efficient expression of the second cistron seemed to occur when the stop codon that terminates translation of the first cistron is located 3' to the Shine-Dalgarno sequence and close to the AUG start codon for the second cistron. When the Shine-Dalgarno sequence was deleted from the first cistron, no detectable expression of the second cistron was observed. This two-cistron system has been used to express the gene encoding methionylalanyl bovine growth hormone with its native codons and the gene encoding methionyl human growth hormone at a level greater than 20% of total cell protein. In the case of human growth hormone, we show that the amount of gene product is not significantly diminished by placing a "functional" first cistron in front of a gene that can be expressed without a cistron.
Are side-chain oxidized oxysterols regulators also in vivo? (2008)

Oxsterols are oxygenated metabolites of cholesterol that are short-lived intermediates or end products in cholesterol excretion pathways. They are present in very low concentrations in mammalian systems, always accompanied by a high excess of cholesterol. According to current concepts, side-chain oxidized oxysterols may be mediators of many cholesterol-induced regulatory effects. When added to cultured cells in vitro, side-chain oxidized oxysterols limit intracellular cholesterol levels by at least three different mechanisms: 1) binding to Insig with subsequent block of the sterol regulatory element-binding proteins (SREBP)-mediated mechanism for regulation of sterol sensitive genes; 2) increasing degradation of hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase, eventually by a mechanism involving binding of Insig to the enzyme; 3) activation of LXR-mediated stimulation of cholesterol transporters and cholesterol metabolism. Addition of pure unesterified oxysterols to cell cultures is however highly unphysiological, and the in vivo relevance of such experiments is questionable. Transgenic mouse models with markedly reduced or increased concentration of some specific oxysterols do not present marked disturbances in cholesterol turnover and homeostasis. Oxysterol-binding proteins such as LXR have been conclusively shown to be of importance for cholesterol turnover in vivo, but their physiological ligands have not yet been defined with certainty. During the last few years, new experimental data has accumulated supporting the contention that side-chain oxysterols are involved in some LXR-mediated regulation in vivo, at least in some biological systems. The new findings will be critically reviewed here.
Asymmetric Steering of Oxa Diels–Alder Reactions with Silyloxydienes Employing Proline Esters as Chiral Auxiliary Groups (1999)

Chiral aldehydes 4a,b, obtained by the ozonolysis of the corresponding N,N′-fumaroylbis[(S)-proline esters] 3 react in the presence of lanthanoid chelate complexes Eu(fod)3 or Eu(hfc)3 with silyloxydienes 7 or 12 to give δ-lactones 10 or dihydro-γ-pyrones 13 with very high diastereomeric ratios (up to 99:1). The absolute configuration of the predominating diastereoisomer of compound 10b was unequivocally determined by X-ray structural analysis.
High Charge Mobility in a Tetrathiafulvalene-Based Microporous Metal–Organic Framework (2012)

The tetratopic ligand tetrathiafulvalene-tetrabenzoate (H4TTFTB) is used to synthesize Zn2(TTFTB), a new metal-organic framework that contains columnar stacks of tetrathiafulvalene and benzoate-lined infinite one-dimensional channels. The new MOF remains porous upon desolvation and exhibits charge mobility commensurate with some of the best organic semiconductors, confirmed by flash-photolysis-time-resolved microwave conductivity measurements. Zn2(TTFTB) represents the first example of a permanently porous MOF with high charge mobility and may inspire further exploration of the electronic properties of these materials.
Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual (1998)

Genetic nomenclature. Looking at yeast cells. Experiments: isolation and characterization of auxotrophic, temperature-sensitive, and UV-sensitive mutants meiotic mapping mitotic recombination and random spore analysis transformation of yeast cytoduction and karyogamy gene replacement isolation of ras2 suppressors manipulating cell types isolation of suppressors of telomeric silencing by insertional shuttle mutagenesis lacZ gene fusion expression in yeast immunofluorescent staining of yeast cells. Techniques and protocols: super-efficient transformation of yeast with lithium acetate/SS-DNA/PEG other yeast transformation methods yeast colony hybridization yeast DNA isolations yeast protein extracts yeast RNA isolation northern analysis - formaldehyde agarose gel, blotting, and hybridization to filters alkaline southern blotting procedure scoring killer factor hydroxylamine mutagenesis of plasmid DNA assay of galactosidase in yeast transformation of bacteria (CaC12 method) plate assay for carboxypeptidase western blotting protein extracts random spore analysis yeast vital strains yeast immunofluorescence with antibodies actin staining in fixed cells PCR analysis of genotype quick e coli plasmid DNA mini-prep. Appendices: media stock preservation yeast genetic map grids electrophoretic karotypes of strains for southern blot mapping strains counting yeast cells with a standard hemocytometer chamber.
Autonomic management of J2EE edge servers (2005)

Nowadays, one of the biggest challenges for companies is to cope with the high cost of their information technologies infrastructure. Edge computing is a new computing paradigm designed to allocate on-demand computing and storage resources. Those resources are web cache servers scattered over the ISP backbones. We argue that this paradigm could be applied for on-demand full application hosting, helping reduce cost. In this paper, we present a J2EE dynamic server able to deploy/host J2EE applications on demand and its autonomic manager. For that, we reengineer and experiment JOnAS, an open-source J2EE static server. Two management policies of the autonomic manager were stressed by a simulation of a worldwide ISP network.
Isolation and expression of cDNA clones encoding a human receptor for IgG (Fc gamma RII). (1987)

We have cloned and expressed a cDNA encoding a human receptor for IgG (Fc gamma R) from the monocyte cell line U937. The deduced structure is a 35-kD transmembrane protein with homology to the mouse Fc[gamma 2b/gamma 1] receptor amino acid sequence of approximately 60% in the extracellular domain. The signal sequence is homologous to the mouse Fc gamma R alpha cDNA clone, while the transmembrane domain shares homology with mouse Fc gamma R beta cDNAs. The cytoplasmic domain is apparently unique. The extracellular domain shows significant homology to proteins of the Ig gene superfamily, including the human c-fms protooncogene/CSF-1 receptor. Mouse Ltk- cells transfected with the human Fc gamma R cDNA express a cell-surface receptor that selectively binds human IgG and is recognized by the anti-Fc gamma RII mAb IV.3. Antibodies against peptides derived from the human Fc gamma R sequence specifically stain U937 cells, but not an Fc gamma RII-bearing B-lymphoblastoid cell line (Daudi). These results identify the human Fc gamma RII as the homologue of mouse Fc[gamma 2b/gamma 1] R, and provide evidence for heterogeneity of Fc gamma RII expressed on monocytes and B cells.
A Phase I and Pharmacokinetic Study of Subcutaneously-Administered Recombinant Human Interleukin-4 (rhuIL-4) in Patients with Advanced Cancer (2000)

rhuIL-4 up to 5.0 microg/kg/day is well tolerated when given by subcutaneous injection. Biologically relevant serum IL-4 levels can be achieved and sustained for at least 8 h after a single injection.
Practical implementation of joint multitarget probabilities (1998)

A Joint Multitarget Probability (JMP) is a posterior probability density p<SUB>T</SUB>(x<SUB>1</SUB>,...,x<SUB>T</SUB>Z) that there are T targets (T an unknown number) with unknown locations specified by the multitarget state X equals (x<SUB>1</SUB>,...,x<SUB>T</SUB>)<SUP>T</SUP> conditioned on a set of observations Z. This paper presents a numerical approximation for implementing JMP in detection, tracking and sensor management applications. A problem with direct implementation of JMP is that, if each x<SUB>t</SUB>, t equals 1,...,T, is discretized on a grid of N elements, N<SUP>T</SUP> variables are required to represent JMP on the T-target sector. This produces a large computational requirement even for small values of N and T. However, when the sensor easily separates targets, the resulting JMP factorizes and can be approximated by a product representation requiring only O(T<SUP>2</SUP>N) variables. Implementation of JMP for multitarget tracking requires a Bayes' rule step for measurement update and a Markov transition step for time update. If the measuring sensor is only influenced by the cell it observes, the JMP product representation is preserved under measurement update. However, the product form is not quite preserved by the Markov time update, but can be restored using a minimum discrimination approach. All steps for the approximation can be performed with O(N) effort. This notion is developed and demonstrated in numerical examples with at most two targets in a 1-dimensional surveillance region. In this case, numerical results for detection and tracking for the product approximation and the full JMP are very similar.