gebhart/patrepeval huggingface dataset:
DELIVERY OF WHOLE LIVER-EQUIVALENT HEPATOCYTE MASS USING POLYMER DEVICES AND HEPATOTROPHIC STIMULATION (1993)

Using hepatocytes injected into prevascularized polymer sponge devices, we studied hepatocyte survival and function after delivery of a whole liver-equivalent of cells into rats. LEW rats and enzyme-deficient Gunn rats served as recipients, respectively. Totally, 28.5 cm2 (0.5-cm thick) of polyvinyl alcohol sponges were implanted per animal. Hepatotrophic stimulation was induced by portacaval shunt and partial (70% or 30%) hepatectomy. Recipient rats received 5 x 10(8) hepatocytes (equivalent to whole rat liver) which were harvested from LEW and Wistar donors, respectively. After engraftment, histologic examination revealed hepatocyte remodeling in the device with capillaries lining plates of hepatocytes, and also tubular structures resembling early biliary radicles. BrdU staining revealed DNA synthesis in hepatocytes, providing evidence of regeneration within the grafts. Quantification of viable hepatocyte area at various time points was performed using computer-assisted morphometry. We then estimated a range of cell numbers from the quantitated cell area. The number of hepatocytes viable at day 7 was estimated at 27.5-46.0% and 6.6-11.0% in the mesentery and subcutaneous site, respectively. Thus the average number was estimated between 10.8% and 18.0% of initially injected hepatocytes. In the Gunn rat experiment, experimental rats that received normal Wistar hepatocytes showed a significantly greater decrease in total serum bilirubin compared with the concurrent control Gunn rats (P < 0.01). At week 1, serum bilirubin in experimental rats decreased to 74.7% (6.80 +/- 0.46 mg/dl) of pretransplantation level (9.10 +/- 0.47 mg/dl) and this was 71.4% of the control rats' bilirubin level (9.53 +/- 0.37 mg/dl). In conclusion, a hepatocyte mass equivalent to a whole rat liver can be delivered into prevascularized polymer sponge devices. At day 7 between 10.8% and 18.0% of these hepatocytes were estimated to be engrafted and functioning. Further optimization of this technique is necessary before clinical application is considered.
TNF-α Induction of CD44-Mediated Leukocyte Adhesion by Sulfation (1998)

Regulation of cell adhesion is important for immune system function. CD44 is a tightly regulated cell adhesion molecule present on leukocytes and implicated in their attachment to endothelium during an inflammatory immune response. The proinflammatory cytokine tumor necrosis factor-alpha, but not interferon-gamma, was found to convert CD44 from its inactive, nonbinding form to its active form by inducing the sulfation of CD44. This posttranslational modification was required for CD44-mediated binding to the extracellular matrix component hyaluronan and to vascular endothelial cells. Sulfation is thus a potential means of regulating CD44-mediated leukocyte adhesion at inflammatory sites.
Record low-loss hybrid rib/wire waveguides for silicon photonic circuits (2010)

Abstract: We report a very low-loss hybrid silicon waveguide circuit consisting of straight rib sections with a propagation loss of 0.272±0.012 dB/cm and compact photonic wire bends of 5µm radius with a loss of 0.0273±0.0004 dB/90 o bend. I.
The Expression of Matrix Metalloproteinase-12 by Oligodendrocytes Regulates Their Maturation and Morphological Differentiation (2004)

Matrix metalloproteinases (MMPs) have important roles in many processes of the developing CNS requiring proteolytic activity such as the migration of neuronal precursors, axonal outgrowth, and vascularization. Another developmental event involving proteolysis is myelin formation, whereby the extensive processes elaborated from oligodendrocytes (OLs) enwrap axons. Here we find MMP-12 transcripts to be produced by OLs in much higher levels than other MMP members examined. MMP-12 activity correlated with the ability of OLs to extend processes in vitro, suggesting a role for MMP-12 in the morphological differentiation of OLs. This was corroborated by results that OL lineage cells from MMP-12 null mice were retarded in their ability to differentiate morphologically and that this deficiency was overcome by the exogenous addition of active MMP-12. Finally, the maturation of oligodendrocyte precursor cells (OPCs) to OLs was significantly reduced in cultures from MMP-12 null mice compared with wild-type controls. We conclude that OL lineage cells express MMP-12 during their maturation and that MMP-12 activity has functional involvement both in maturation of OPCs and in the ability of OPCs and OLs to extend processes.
Fibromodulin Suppresses Nuclear Factor-κB Activity by Inducing the Delayed Degradation of IKBA via a JNK-dependent Pathway Coupled to Fibroblast Apoptosis (2010)

Fibulin-5 (FBLN5) belongs to the Fibulin family of secreted extracellular matrix proteins, and our laboratory first established FBLN5 as a novel target for TGF-β in fibroblasts and endothelial cells. To better understand the pathophysiology of FBLN5, we carried out microarray analysis to identify fibroblast genes whose expressions were regulated by FBLN5 and TGF-β. In doing so, we identified fibromodulin (Fmod) as a novel target gene of FBLN5, and we validated the differential expression of Fmod and 12 other FBLN5-regulated genes by semi-quantitative real time PCR. Fmod belongs to the small leucine-rich family of proteoglycans, which are important constituents of mammalian extracellular matrices. Interestingly, parental 3T3-L1 fibroblasts displayed high levels of nuclear factor-κB (NF-κB) activity, although those engineered to express Fmod constitutively exhibited significantly reduced NF-κB activity, suggesting that Fmod functions to inhibit NF-κB signaling. By monitoring alterations in the activation of NF-κB and the degradation of its inhibitor, IκBα, we demonstrate for the first time that Fmod contributes to the constitutive degradation of IκBα protein in 3T3-L1 fibroblasts. Mechanistically, we observed Fmod to delay the degradation of IκBα by promoting the following: (i) activation of c-Jun N-terminal kinase; (ii) inhibition of calpain and casein kinase 2 activity; and (iii) induction of fibroblast apoptosis. Taken together, our study identified a novel function for Fmod in directing extracellular signaling, particularly the regulation of NF-κB activity and cell survival.
Difference in Th1 and Th17 Lymphocyte Adhesion to Endothelium (2012)

T cell subset-specific migration to inflammatory sites is tightly regulated and involves interaction of the T cells with the endothelium. Th17 cells often appear at different inflammatory sites than Th1 cells, or both subsets appear at the same sites but at different times. Differences in T cell subset adhesion to endothelium may contribute to subset-specific migratory behavior, but this possibility has not been well studied. We examined the adhesion of mouse Th17 cells to endothelial adhesion molecules and endothelium under flow in vitro and to microvessels in vivo and we characterized their migratory phenotype by flow cytometry and quantitative RT-PCR. More Th17 than Th1 cells interacted with E-selectin. Fewer Th17 than Th1 cells bound to TNF-α-activated E-selectin-deficient endothelial cells, and intravital microscopy studies demonstrated that Th17 cells engage in more rolling interactions with TNF-α-treated microvessels than Th1 cells in wild-type mice but not in E-selectin-deficient mice. Th17 adhesion to ICAM-1 was dependent on integrin activation by CCL20, the ligand for CCR6, which is highly expressed by Th17 cells. In an air pouch model of inflammation, CCL20 triggered recruitment of Th17 but not Th1 cells. These data provide evidence that E-selectin- and ICAM-1-dependent adhesion of Th17 and Th1 cells with endothelium are quantitatively different.
Cartesian K-Means (2013)

A fundamental limitation of quantization techniques like the k-means clustering algorithm is the storage and run-time cost associated with the large numbers of clusters required to keep quantization errors small and model fidelity high. We develop new models with a compositional parameterization of cluster centers, so representational capacity increases super-linearly in the number of parameters. This allows one to effectively quantize data using billions or trillions of centers. We formulate two such models, Orthogonal k-means and Cartesian k-means. They are closely related to one another, to k-means, to methods for binary hash function optimization like ITQ (Gong and Lazebnik, 2011), and to Product Quantization for vector quantization (Jegou et al., 2011). The models are tested on large-scale ANN retrieval tasks (1M GIST, 1B SIFT features), and on codebook learning for object recognition (CIFAR-10).
Hepatoma-Derived Growth Factor-Related Protein-3 Is a Novel Angiogenic Factor (2015)

Hepatoma-derived growth factor-related protein-3 (Hdgfrp3 or HRP-3) was recently reported as a neurotrophic factor and is upregulated in hepatocellular carcinoma to promote cancer cell survival. Here we identified HRP-3 as a new endothelial ligand and characterized its in vitro and in vivo functional roles and molecular signaling. We combined open reading frame phage display with multi-round in vivo binding selection to enrich retinal endothelial ligands, which were systematically identified by next generation DNA sequencing. One of the identified endothelial ligands was HRP-3. HRP-3 expression in the retina and brain was characterized by Western blot and immunohistochemistry. Cell proliferation assay showed that HRP-3 stimulated the growth of human umbilical vein endothelial cells (HUVECs). HRP-3 induced tube formation of HUVECs in culture. Wound healing assay indicated that HRP-3 promoted endothelial cell migration. HRP-3 was further confirmed for its in vitro angiogenic activity by spheroid sprouting assay. HRP-3 extrinsically activated the extracellular-signal-regulated kinase ½ (ERK1/2) pathway in endothelial cells. The angiogenic activity of HRP-3 was independently verified by mouse cornea pocket assay. Furthermore, in vivo Matrigel plug assay corroborated HRP-3 activity to promote new blood vessel formation. These results demonstrated that HRP-3 is a novel angiogenic factor.
EFFECT OF THE α-LINOLENIC ACID ENRICHED DIET ON ATOPIC DERMATITIS. (1992)

Six outpatients suffering from atopic dermatitis underwent the α-linolenic acid enriched diet, that is, reducing n-6 series polyunsaturated fatty acid (linoleic acid, arachidonic acid [AA]) intake and increasing n-3 series polyunsaturated fatty acid (α-linolenic acid, eicosapentaenoic acid [EPA]) intake using perilla oil to suppress the release of chemical mediator derived from arachidonic acid cascade. After 124±40.4 (mean±SD) days of this treatment, improvement of the dermatitis was observed in three patients, and in another one patient by continuing the diet after this study. In the phospholipid fraction in their serum, the n-3/n-6 ratio and the EPA/AA ratio were significantly increased (p<0.01). According to that, Leukotriene C4 release from polymorphonuclear leukocytes by zymosan and fresh autologous serum was significantly decreased (p<0.05). This results suggest that daily meals consist in the α-linolenic acid enriched diet have some benefit in the treatment of allergic diseases.
Multi-valued logic synthesis (1999)

We survey some of the methods used for manipulating, representing, and optimizing multi-valued logic with the view of both building a better understanding of the more specialized binary-valued logic, as well as motivating research towards a true multi-valued multi-level optimization package.
Abandoned therapies and unpublished trials in rheumatoid arthritis (2003)

The capability of selectively targeting pathogenic elements of disease with biologic therapies has created a new therapeutic repertoire. Although a substantial number of biologic agents have been developed for treatment of rheumatoid arthritis, few have been approved for use. Most of the agents have failed to reach the approval stage because of inadequate clinical benefit. Despite this, studies of these agents have provided extremely valuable lessons in study design, immunobiology, pharmacodynamic evaluation, and the utility of animal models in the development of biologic agents. These insights have laid the groundwork for future development of other novel therapeutic agents in the treatment of rheumatoid arthritis.
Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. (1984)

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.
Hypotensive Activity of Terpenes Found in Essential Oils (2010)

The cardiovascular activity of essential oils has been reported. Some studies showed that the main chemical components of these oils contribute to their pharmacological activity. Therefore, the cardiovascular activity of four monoterpenes and one sesquiterpene was evaluated in the present work. In non-anaesthetized normotensive rats, (+)-alpha-pinene, (-)-beta-pinene, (+/-)-citronellol and (+/-)-linalool (1, 5, 10, and 20 mg/kg, i.v.) induced hypotension [maximal effect: (-35 +/- 3)%, (-46 +/- 4)%, (-48 +/- 2)% and (-40 +/- 2)%, respectively; n=6] and tachycardia [maximal effect: (13 +/- 4)%, (16 +/- 7)%, (21 +/- 1)% and (19 +/- 3)%, respectively; n=6] while (-)-a-bisabolol (1, 5, 10, and 20 mg/kg, i.v.) induced hypotension [maximal effect: (-47 +/- 8)%, n=6] and bradycardia [maximal effect: (-57 +/- 3)%]. In conclusion, these results demonstrated that all terpenes tested had hypotensive activity in rats and that the pharmacological effect of the terpene alcohols was more effective than that of the terpene hydrocarbons.
Mast‐cell‐deficient W/Wv mice exhibit A decreased rate of tumor angiogenesis (1988)

The role of host mast cells in tumor-associated angiogenesis was investigated by comparing the angiogenic response of genetically mast-cell-deficient W/Wv mice and mast-cell-sufficient +/+ littermate mice to s.c. growing B16-BL6 tumors. The angiogenic response was found to be slower and initially less intense in W/Wv mice than in +/+ mice. Fewer W/Wv mice than +/+ mice developed spontaneous lung metastases and W/Wv mice exhibited fewer lung metastases per mouse. Bone-marrow repair of the mast-cell deficiency restored the angiogenic response of W/Wv mice and also restored the incidence of hematogenous metastases to approach that of +/+ mice. Differences in lymphatic metastasis were not detected between W/Wv and +/+ mice. These results demonstrate a role for mast cells in vivo during tumor angiogenesis, and suggest a role also for host mast cells in hematogenous metastasis.
Lymphaticovenous differentiation in Kaposi's sarcoma. Cellular phenotypes by stage. (1988)

The histogenesis of Kaposi's sarcoma was investigated by immunohistochemical staining of 20 skin specimens that represented four main histologic stages. The early phase of Stage 1 contained lymphatic-like clefts lined by endothelial cells with thin, discontinuous basement membranes shown by anti-laminin, absent Factor VIII-related antigen reactivity (FVIIIRAg), and only rare staining with dilute Ulex europaeus agglutinin I (UEA-I). In the late phase of Stage 1, the clefts developed into anastomosing, blood-filled channels, and the basement membrane became complete. Endothelial marker reactivities were not definitive, but weak staining with UEA-I and variable staining for FVIIIRAg characterized the spindle cells of Stage 2. However, spindle cells in monomorphic nodules were individually enclosed by immunoreactive laminin. The sequence of events, particularly in light of previous angiographic findings of lymphaticovenous union, suggests a disturbance in lymphaticovenous differentiation in Kaposi's sarcoma. Sclerotic closure of channels unable to maintain competent blood flow may select against lymphendothelial traits in the developing spindle cell nodule.
Dinuclear metal catalysts: improved performance of heterodinuclear mixed catalysts for CO2–epoxide copolymerization (2014)

Some of the most active catalysts for carbon dioxide and epoxide copolymerization are dinuclear metal complexes. Whilst efficient homodinuclear catalysts are known, until now heterodinuclear catalysts remain unreported. Here, a facile, in situ route to a catalyst system comprising a mixture of homo- and heteronuclear Zn-Mg complexes is presented. This catalyst system shows excellent polymerization control and exhibits significantly higher activity than the homodinuclear catalysts alone or in combination.
Peroxisome proliferator‐activated receptor‐α agonist, Wy 14 643, improves metabolic indices, steatosis and ballooning in diabetic mice with non‐alcoholic steatohepatitis (2011)

Pharmacological activation of PPARα improves metabolic milieu, steatosis, ballooning, and combats NF-κB and JNK activation, neutrophil and F4/80 macrophage recruitment in diabetes-related NASH. However, persistent liver inflammation with high serum MCP1 due to unsuppressed adipose inflammation may limit PPARα agonists' efficacy as therapy for NASH.
Photoswitching Using Visible Light: A New Class of Organic Photochromic Molecules (2014)

A versatile new class of organic photochromic molecules that offers an unprecedented combination of physical properties including tunable photoswitching using visible light, excellent fatigue resistance, and large polarity changes is described. These unique features offer significant opportunities in diverse fields ranging from biosensors to targeted delivery systems while also allowing non-experts ready synthetic access to these materials.
The WINTERP Widget INTERPreter—a Lisp prototyping and extension environment for OSF/motif based applications user-interfaces (1990)

Winterp is an interactive, language-based user-interface and application-construction environment enabling rapid prototyping of applications with graphical user interfaces based on the OSF/Motif UI Toolkit. Winterp also serves as a customization environment for delivered applications by providing a real programming language as an extension language. Many existing user-interface languages only have the expressive power to describe static layout of user interface forms; by using a high-level language for extensions and prototyping, Winterp also handles the dynamic aspects of UI presentation, e.g. the use of direct manipulation, browsers, and dialog. Winterp makes rapid prototyping possible because its language is based on an interpreter, thereby enabling interactive construction of application functionality and giving immediate feedback on incremental changes. Winterp's language is based on David Betz's public domain Xlisp interpreter which features a subset of Common Lisp's functionality. The language is extensible, permitting new Lisp primitives to be added in the C language and allowing hybrid implementations constructed from interpreted Lisp and compiled C. Hybrid implementation gives Winterp -based applications the successful extension and rapid-prototyping capabilities of Lisp-based environments, while delivering the multiprocessing perfor- mance of C applications running on personal Unix workstations.
Bioinspired Energy Conversion Systems for Hydrogen Production and Storage (Eur. J. Inorg. Chem. 9/2008) (2008)

Abstract The cover picture shows the photocatalytic production of hydrogen and the interconversion between hydrogen and formic acid with the use of catalysts. These processes have become important in the search for alternative energy resources that are renewable, in order to combat global environmental problems. Hydrogen is a clean energy source, and therefore its production and storage is a key to the future. Details are presented in the Microreview by S. Fukuzumi on p. 1351 ff.
bibliography of liquid cooled heat sinks for thermal enhancement of electronic packages (1999)

A bibliography of 225 publications dealing with liquid cooled heat sinks for thermal enhancement of electronic packages is presented. The bibliography also includes papers dealing with design and performance analysis of heat sinks and extended surfaces cooled with liquids. The papers are arranged in several categories.
Rate of Return on Differential Premiums for Selected Participating Life Insurance Contracts (1983)

Phyllis Schiller Myers, S. Travis Pritchett, Rate of Return on Differential Premiums for Selected Participating Life Insurance Contracts, The Journal of Risk and Insurance, Vol. 50, No. 4 (Dec., 1983), pp. 569-586
Emergency Presentations to Northern Territory Public Hospitals: Demand and Access Analysis (2003)

This study aims to quantify trends in emergency presentations to Northern Territory (NT) public hospitals over thepast five years with respect to demand and access. Retrospective analysis was undertaken on data extracted from theNT Module of Caresys and the Hospital Morbidity Data System. There has been a 4.6% decrease in presentations tothe five public hospitals between 1996 and 2001 compared to a 9.4% growth in the population. Despite the apparentdecline in total presentations, the acuity of patients has increased dramatically over the same period. Access analysis ofpresentations seen within the recommended triage time revealed considerable variability, especially for triage categories2, 3 and 4. The access block problem, discrepancies in recorded waiting time and irregularities among the regionalhospitals within the triage system have also been identified. Recommendations including improved access to inpatientbeds and admission to wards were developed in response to the changing role of the emergency department.
Electrostatic Adsorption of Polystyrene Nanospheres onto the Surface of an Ultrathin Polymer Film Prepared by Using an Alternate Adsorption Technique (1998)

We studied the adsorption of polystyrene nanospheres that have anionic charges on their surfaces from their aqueous dispersions onto the surfaces of cationic ultrathin polymer films quantitatively and kinetically by using a quartz crystal microbalance (QCM) and scanning electron microscopy (SEM). The polymer films were successively prepared by the alternate adsorption of poly(allylamine hydrochloride) (PAH) and poly(sodium 4-styrenesulfonate) (PSS) in the absence or presence of suitable NaCl concentrations onto the QCM substrate so that we could observe their formation process by its frequency shifts. We observed the specific adsorption of the nanospheres by means of electrostatic interaction onto the outermost PAH surface by QCM as well. The dependence of their adsorption amounts against the NaCl concentration in polymer precursor film preparation was a sigmoid curve, indicating that there is a critical charge on/in the film for their adsorption. SEM observations showed monolayer adsorption without three-dimensional aggregation. We also studied the effects of particle size on their adsorption behavior and the possibility of the multilayering of nanospheres. This research on adsorption behavior by electrostatic interaction will be applied to the adsorption system of not only polymeric nanospheres but also other inorganic nanoparticles.