gebhart/patrepeval huggingface dataset:
Cross-protection of mice immunized with different influenza A (H2) strains and challenged with viruses of the same HA subtype. (1997)

Cross-protection of mice immunized with inactivated preparations of human and avian influenza A (H2) viruses was determined after lethal infection with mouse-adapted (MA) variants of human A/Jap x Bell/57 (H2N1) and avian A/NJers/78 (H2N3) viruses. The MA variants differed from the original strains by acquired virulence for mice and changes in the HA antigenicity. These studies indicated that mice vaccinated with human influenza A (H2) viruses were satisfactorily protected against challenge with A/Jap x Bell/57-MA variant; the survival rate was in the range of 61%-88.9%. Immunization of mice with the same viral preparations provided lower levels of protection against challenge with A/NJers/78-MA variant. Vaccination of mice with the avian influenza A (H2) viruses induced better protection than with human strains against challenge with both MA variants. Challenge with A/NJers/78-MA variant revealed that 76.2%-95.2% of animals were protected when vaccinated with avian influenza virus strains isolated before 1980, and that the protection reached only 52.4%-60.0% in animals vaccinated with strains isolated in 1980-1985. The present study revealed that cross-protection experiments in a mouse model could provide necessary information for the development of appropriate influenza A (H2) virus vaccines with a potential for these viruses to reappear in a human population.
Redirecting NK cells mediated tumor cell lysis by a new recombinant bifunctional protein (2008)

Natural killer (NK) cells are at the crossroad between innate and adaptive immunity and play a major role in cancer immunosurveillance. NK cell stimulation depends on a balance between inhibitory and activating receptors, such as the stimulatory lectin-like receptor NKG2D. To redirect NK cells against tumor cells, we designed bifunctional proteins able to specifically bind tumor cells and to induce their lysis by NK cells, after NKG2D engagement. To this aim, we used the 'knob into hole' heterodimerization strategy, in which 'knob' and 'hole' variants were generated by directed mutagenesis within the CH3 domain of human IgG1 Fc fragments fused to an anti-CEA or anti-HER2 scFv or to the H60 murine ligand of NKG2D, respectively. We demonstrated the capacity of the bifunctional proteins produced to specifically coat tumor cells surface with H60 ligand. Most importantly, we demonstrated that these bifunctional proteins were able to induce an NKG2D-dependent and antibody-specific tumor cell lysis by murine NK cells. Overall, the results show the possibility to redirect NK cytotoxicity to tumor cells by a new format of recombinant bispecific antibody, opening the way of potential NK cell-based cancer immunotherapies by specific activation of the NKG2D receptor at the tumor site.
FGF-2 Inhibits Osteogenesis in Mouse Adipose Tissue-Derived Stromal Cells and Sustains their Proliferative and Osteogenic Potential State (2006)

In addition to adult bone marrow-derived mesenchymal stem cells, more recently, a second large stromal compartment found in adipose tissue has received attention and is believed to contain multipotent cells. In vitro, adipose tissue-derived stromal cells (ADS) can differentiate down osteogenic, chondrogenic, myogenic, adipose, and even neuronal pathways. In this article, we explore the effect of fibroblast growth factors 2 (FGF-2) on ADS cells. Our results demonstrated that FGF-2 inhibits osteogenesis in ADS cells. The osteogenic inhibitory effects is dose-dependent and reversible, thus suggesting that the lack of osteogenesis observed in ADS cells exposed to FGF-2 is not due to a negative selection triggered by this factor on a subpopulation of osteoblast progenitors. Furthermore, either overexpression of FGF-2, or continuous FGF-2 treatment sustain the proliferative and osteogenic potential state of ADS cells. Therefore, FGF-2 appears to be a positive regulator of osteoprogenitor cells and a negative regulator of osteoblast differentiation in ADS cells. These FGF-2 functional characteristics may assist with cell selection and enrichment for the purpose of bone tissue engineering.
Motion-based machine vision techniques for the management of large crowds (2003)

The early detection and the prevention of crowd-related emergencies are the main aims of large crowd management Closed Circuit Television (CCTV) systems. However, environmental and psychological factors play a role in degrading the performance of CCTV operators, hence causing a threat to crowd safety. We present computer vision techniques that estimate the paths and directions of crowd flows in CCTV images and improve the perception of scene dynamics by offering online illustrations. Moreover, we present motion-based algorithms to detect crowd-related emergencies and assist CCTV operators in ensuring crowd safety.
Authentication and authorization techniques in distributed systems (1993)

The authors consider the following techniques for performing the authentication of user identity and the granting of proper authorization in distributed environments: user login and routing constraints at the network level; special-purpose, challenge-response systems; private key based systems such as Kerberos; and public key based systems with smart cards. They describe and discuss the strengths and weaknesses (i.e., residual vulnerabilities) of each technique and provide guidance with respect to their applicability and deployment.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Preparation of CAD model for finite element analysis (2010)

In spite of the widespread use of Computer Aided Design (CAD) systems for design and Computer Aided Analysis (CAE) systems for analysis, the two processes are not well integrated because CAD and CAE models inherently use different types of geometric models and there currently exists no generic, unified model that allows both design and analysis information to be specified and shared. In this paper, finite element-based model transformation method is proposed to realize information mapping from CAD to CAE. The element and node information is abstracted from finite element model and transformed to CAE system. The finite element model is prepared in CAD system which involves feature recognition and detail removal. In order to make the analysis conforms to practical needs and improve computational efficiency, additional operations are added in local regions before mesh generation to control local mesh density.
Community-based Random Access Scheme for M2M Communications in LTE Networks (2019)

Supporting Machine-to-Machine (M2M) communications has been an essential requirement for the Long Term Evolution (LTE) networks. To cope with the massive access requests with stringent delay requirements, in this paper, a community-based random access scheme is proposed by exploiting the spatial correlation of Machine-Type Devices (MTDs). Specifically, a community detection algorithm is first proposed to form the community structure of the network, based on which one MTD is selected in each community to establish a connection with the base station upon each emergency event. Simulation results corroborate that by reducing the number of access requests, the proposed scheme can significantly improve the delay performance especially in the massive access scenarios with a limited number of preambles.
An architecture for addition and subtraction of long word length numbers in the logarithmic number system (1990)

An architecture is described for performing addition and subtraction of numbers in the logarithmic number system using small lookup tables. Previous implementations require approximately 4*2/sup F/ words for F bits of precision in the fraction. The author shows how to reduce the size of the lookup table to fewer than (18+F)*2/sup F/2/ words. The key to this is the use of a linear approximation of the nonlinear functions stored in the lookup tables. The functions involved are highly nonlinear in some regions, so variable size regions are used for the approximation. The replacement of the F exponential dependence on the number of words by F/2 allows roughly 50% more bits of fractional precision to be obtained for a given amount of ROM. The architecture is mathematically analyzed, yielding explicit expressions for all design parameters. The approach is illustrated with an example logarithmic addition and subtraction unit using 32-b words with 30-b exponents containing 22 fractional bits. A factor of 118 reduction in table size compared to previous techniques is achieved for this example.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
The 15-lipoxygenase-1 product 13- S -hydroxyoctadecadienoic acid down-regulates PPAR-δ to induce apoptosis in colorectal cancer cells (2003)

Diminished apoptosis, a critical event in tumorigenesis, is linked to down-regulated 15-lipoxygenase-1 (15-LOX-1) expression in colorectal cancer cells. 13-S-hydroxyoctadecadienoic acid (13-S-HODE), which is the primary product of 15-LOX-1 metabolism of linoleic acid, restores apoptosis. Nonsteroidal antiinflammatory drugs (NSAIDs) transcriptionally up-regulate 15-LOX-1 expression to induce apoptosis. Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors for linoleic and arachidonic acid metabolites. PPAR-delta promotes colonic tumorigenesis. NSAIDs suppress PPAR-delta activity in colon cancer cells. The mechanistic relationship between 15-LOX-1 and PPAR-delta was previously unknown. Our current study shows that (i) 13-S-HODE binds to PPAR-delta, decreases PPAR-delta activation, and down-regulates PPAR-delta expression in colorectal cancer cells; (ii) the induction of 15-LOX-1 expression is a critical step in NSAID down-regulation of PPAR-delta and the resultant induction of apoptosis; and (iii) PPAR-delta is an important signaling receptor for 13-S-HODE-induced apoptosis. The in vivo relevance of these mechanistic findings was demonstrated in our tumorigenesis studies in nude mouse xenograft models. Our findings indicate that the down-regulation of PPAR-delta by 15-LOX-1 through 13-S-HODE is an apoptotic signaling pathway that is activated by NSAIDs.
[Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria]. (1997)

GPI anchored protein assay provided sensitive and specific method for the diagnosis of PNH.
Augmentation of Macular Pigment following Implantation of Blue Light–Filtering Intraocular Lenses at the Time of Cataract Surgery (2009)

Customized HFP can reliably measure the MPOD spatial profile in the presence of lens opacity, and cataract surgery does not artifactually alter MPOD readings. This study also provides evidence that implanting an IOL that filters blue light is associated with augmentation of MPOD in the absence of raised serum concentrations of L and Z. However, further and longitudinal study is needed to assess whether the observed increase in MPOD after implantation of blue-filtering IOLs is associated with reduced risk of AMD development and/or progression.
Mouse Trophoblast Stem Cells (2005)

The trophectoderm is one of the earliest cell types to differentiate in the forming mammalian embryo. It is responsible for the initial implantation and the formation of the trophoblast components of the placenta, an organ essential for nutrient and waste exchange between the fetus and its mother. The trophoblast can be modeled in vitro using trophoblast stem cells. Trophoblast stem cells require fibroblast growth factor (FGF)4, heparin, and contact with embryonic fibroblasts, or fibroblast-conditioned medium. They grow as tight epithelial colonies, which express markers of the early trophectoderm and have been shown to contribute to all of the components of the placenta through chimera studies. These cells can be passaged indefinitely and can be differentiated by removal of FGF4 and fibroblasts and will express genetic markers of later placental cell types. This chapter will discuss the initial derivation of trophoblast stem cells from the blastocyst stage, maintenance, differentiation, flow cytometry and transfection techniques that can be used with these cells.
H5N1 Influenza — Continuing Evolution and Spread (2006)

We don't know whether the next influenza pandemic will be caused by the H5N1 avian influenza virus. But Drs. Robert Webster and Elena Govorkova write that given the number of cases of H5N1 influenza that have occurred in humans to date (251) and the rate of death of more than 50%, it would be prudent to develop robust plans for dealing with such a pandemic.
Oximes (2007)

This chapter contains sections titled: Introduction History of Oxime Development Mechanism of Oxime Action Kinetics of Oxime-Induced Reactivation of Human Ache In Vitro Efficacy of Oximes Against Nerve Agents in Animals In Vivo Efficacy of Oximes Against Nerve Agents in Humans In Vivo Stability of Oximes and Pharmaceutical Aspects Metabolism and Excretion Safety and Tolerability of Therapeutic Oximes Assessment of Therapeutic Oxime Effects by Theoretical Models Conclusions References
Minireview: Malonyl CoA, AMP-Activated Protein Kinase, and Adiposity (2003)

An increasing body of evidence has linked AMP-activated protein kinase (AMPK) and malonyl coenzyme A (CoA) to the regulation of energy balance. Thus, factors that activate AMPK and decrease the concentration of malonyl CoA in peripheral tissues, such as exercise, decrease triglyceride accumulation in the adipocyte and other cells. The data reviewed here suggest that this is related to the fact that these factors concurrently increase fatty acid oxidation, decrease the esterification of fatty acids to form glycerolipids, and, by mechanisms still unknown, increase energy expenditure. Malonyl CoA contributes to these events because it is an allosteric inhibitor of carnitine palmitoyltransferase, the enzyme that controls the transfer of long-chain fatty acyl CoA from the cytosol to the mitochondria, where they are oxidized. AMPK activation in turn increases fatty acid oxidation (by effects on enzymes that govern malonyl CoA synthesis and possibly its degradation) and inhibits triglyceride synthesis. It also increases the expression of uncoupling proteins and the transcriptional regulator peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC1alpha), which could possibly increase energy expenditure. Recent studies suggest that the ability of leptin, adiponectin, 5'-aminoimidazole 4-carboxamide riboside (AICAR), adrenergic agonists, and metformin to diminish adiposity may be mediated, at least in part, by AMPK activation in peripheral tissues. In addition, preliminary studies suggest that malonyl CoA and AMPK take part in fuel-sensing and signaling mechanisms in the hypothalamus that could regulate food intake and energy expenditure.
Dilute Magnetic Semiconducting Oxides (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Realizing low loss air core photonic crystal fibers by exploiting an antiresonant core surround (2005)

The modal properties of an air core photonic crystal fiber which incorporates an anti-resonant feature within the region that marks the transition between the air core and the crystal cladding are numerically calculated. The field intensity at the glass/air interfaces is shown to be reduced by a factor of approximately three compared to a fiber with more conventional core surround geometry. The reduced interface field intensity comes at the expense of an increased number of unwanted core interface modes within the band gap. When the interface field intensity is associated with modal propagation loss, the findings are in accord with recent measurements on fabricated fibers which incorporate a similar antiresonant feature.
Synthesis of ω‐hydroxy dodecanoic acid based on an engineered CYP153A fusion construct (2013)

A bacterial P450 monooxygenase-based whole cell biocatalyst using Escherichia coli has been applied in the production of ω-hydroxy dodecanoic acid from dodecanoic acid (C12-FA) or the corresponding methyl ester. We have constructed and purified a chimeric protein where the fusion of the monooxygenase CYP153A from Marinobacter aquaeloei to the reductase domain of P450 BM3 from Bacillus megaterium ensures optimal protein expression and efficient electron coupling. The chimera was demonstrated to be functional and three times more efficient than other sets of redox components evaluated. The established fusion protein (CYP153AM. aq. -CPR) was used for the hydroxylation of C12-FA in in vivo studies. These experiments yielded 1.2 g l(-1) ω-hydroxy dodecanoic from 10 g l(-1) C12-FA with high regioselectivity (> 95%) for the terminal position. As a second strategy, we utilized C12-FA methyl ester as substrate in a two-phase system (5:1 aqueous/organic phase) configuration to overcome low substrate solubility and product toxicity by continuous extraction. The biocatalytic system was further improved with the coexpression of an additional outer membrane transport system (AlkL) to increase the substrate transfer into the cell, resulting in the production of 4 g l(-1) ω-hydroxy dodecanoic acid. We further summarized the most important aspects of the whole-cell process and thereupon discuss the limits of the applied oxygenation reactions referring to hydrogen peroxide, acetate and P450 concentrations that impact the efficiency of the production host negatively.
Adenosine cardioplegia. Adenosine versus potassium cardioplegia: effects on cardiac arrest and postischemic recovery in the isolated rat heart. (1989)

Adenosine is a potential cardioplegic agent by virtue of its specific inhibitory properties on nodal tissue. We tested the hypothesis that adenosine could be more effective than potassium in inducing rapid cardiac arrest and enhancing postischemic hemodynamic recovery. Isolated rat hearts were perfused with Krebs-Henseleit buffer or cardioplegic solutions to determine the time to cardiac arrest and the high-energy phosphate levels at the end of cardioplegia. Cardioplegic solutions contained adenosine 10 mmol/L, potassium 20 mmol/L, or adenosine 10 mmol/L + potassium 20 mmol/L and were infused at a rate of 2 ml/min for 3 minutes at 10 degrees C. Both time taken and total number of beats to cardiac arrest during 3 minutes of cardioplegia were reduced by adenosine 10 mmol/L and adenosine 10 mmol/L + potassium 20 mmol/L when compared with potassium 20 mmol/L alone (p less than 0.001). Tissue phosphocreatine was conserved by adenosine 10 mmol/L when compared with potassium 20 mmol/L, being 7.1 +/- 0.2 (mumol/gm wet weight (n = 7) and 6.0 +/- 0.3 mumol/gm wet weight (n = 5), respectively (p less than 0.05). Postischemic hemodynamic recovery was tested in isolated working rat hearts. After initial cardiac arrest, the cardioplegic solution was removed with Krebs-Henseleit buffer at a rate of 2 ml/min for 3 minutes at 10 degrees C, and thereafter total ischemia was maintained for 30 or 90 minutes at 10 degrees C before reperfusion. Adenosine 10 mmol/L enhanced recovery of aortic output when compared with potassium 20 mmol/L or adenosine 10 mmol/L + potassium 20 mmol/L, the percentage recovery after 30 minutes of ischemia being 103.0% +/- 4.4% (n = 6), 89.0% +/- 5.8% (n = 6), and 86.6% +/- 4.3% (n = 6), respectively (p less than 0.05 for comparison between adenosine 10 mmol/L and potassium 20 mmol/L). Thus adenosine cardioplegia caused rapid cardiac arrest and improved postischemic recovery when compared with potassium cardioplegia and with a combination of these two agents.
Effects of Regiochemical and Stereochemical Errors on the Course of Isotactic Propene Polyinsertion Promoted by Homogeneous Ziegler-Natta Catalysts (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffects of Regiochemical and Stereochemical Errors on the Course of Isotactic Propene Polyinsertion Promoted by Homogeneous Ziegler-Natta CatalystsVincenzo Busico, Roberta Cipullo, John C. Chadwick, Johan F. Modder, and Olof SudmeijerCite this: Macromolecules 1994, 27, 26, 7538–7543Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://pubs.acs.org/doi/10.1021/ma00104a006https://doi.org/10.1021/ma00104a006research-articleACS PublicationsRequest reuse permissionsArticle Views555Altmetric-Citations140LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fast and accurate instruction fetch and branch prediction (1994)

Accurate branch prediction is critical to performance; mispredicted branches mean that ten's of cycles may be wasted in superscalar architectures. Architectures combining very effective branch prediction mechanisms coupled with modified branch target buffers (BTB's) have been proposed for wide-issue processors. These mechanisms require considerable processor resources. Concurrently, the larger address space of 64-bit architectures introduce new obstacles and opportunities. A larger address space means branch target buffers become more expensive. In this paper, we show how a combination of less expensive mechanisms can achieve better performance than BTB's. This combination relies on a number of design choices described in the paper. We used trace-driven simulation to show that our proposed design, which uses fewer resources, offers better performance than previously proposed alternatives for most programs, and indicate how to further improve this design.
The Importance of Strong Carbon−Metal Adhesion for Catalytic Nucleation of Single-Walled Carbon Nanotubes (2007)

Density functional theory is used to show that the adhesion between single-walled carbon nanotubes (SWNTs) and the catalyst particles from which they grow needs to be strong to support nanotube growth. It is found that Fe, Co, and Ni, commonly used to catalyze SWNT growth, have larger adhesion strengths to SWNTs than Cu, Pd, and Au and are therefore likely to be more efficient for supporting growth. The calculations also show that to maintain an open end of the SWNT it is necessary that the SWNT adhesion strength to the metal particle is comparable to the cap formation energy of the SWNT end. This implies that the difference between continued and discontinued SWNT growth to a large extent depends on the carbon-metal binding strength, which we demonstrate by molecular dynamics (MD) simulations. The results highlight that first principles computations are vital for the understanding of the binding strength's role in the SWNT growth mechanism and are needed to get accurate force field parameters for MD.