gebhart/patrepeval huggingface dataset:
Identification of the sterol- and actin-binding domains of plasma vitamin D binding protein (Gc-globulin) (1992)

The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by limited proteolysis, permitted the isolation and identification of three overlapping peptides in the amino terminus of the molecule. When G-actin affinity chromatography was applied to other proteolytic fragments, two fragments from the carboxy terminus of the molecule were isolated and identified. Another, large, tryptic fragment displayed both sterol- and actin-binding properties. The amino-terminal assignment of the sterol-binding domain was confirmed by demonstrating sterol-specific binding by an in vitro transcribed and translated product of a mutated rat DBP cDNA encoding a protein truncated in its carboxy terminus. The sterol-binding domain was localized to the region between the first-amino-terminal disulfide bond, and the actin-binding domain was found between residues 350 and 403. A high degree of sequence conservation in these regions was found among human, rat, and mouse DBP's. These functional domain assignments confirm the apparent independence of these two binding activities and help to explain the observed triprotein complex of DBP-actin-DNase I and the competition between DBP and profilin for G-actin binding. Our findings should facilitate more precise delineation of the binding domains by site-directed mutagenesis experiments.
Electroosmotically Induced Hydraulic Pumping with Integrated Electrodes on Microfluidic Devices (2001)

Electroosmotic manipulation of fluids was demonstrated using thin metal electrodes integrated within microfluidic channels at the substrate and cover plate interface. Devices were fabricated by photolithographically patterning electrodes on glass cover plates that were then bonded to polymeric substrates into which the channels were cast. Polymeric substrates were used to provide a permeable membrane for the transport and removal of gaseous electrolysis products generated at the electrodes. Electroosmotic flow between interdigitated electrodes was demonstrated and provided electric field-free pumping of fluids in sections of the channel outside of the electrode pairs. The resultant pumping velocities were shown to be dependent on the applied voltage, not on the applied field strength, and independent of the length of the electroosmotically pumped region.
Comparison of optical losses in dielectric-apertured vertical-cavity lasers (1996)

The performance of vertical-cavity lasers (VCL's) employing dielectric apertures formed by lateral oxidation or wet-etch undercutting of an AlAs layer on a common substrate are compared. Although both device types performed well, extracted optical losses from the wet-etch undercut structures exceed those of AlAs-oxide apertured lasers. The difference in performance is attributed to optical scattering losses specific to the aperture fabrication method, enhanced by loss from the larger index of refraction discontinuity of the air-gap aperture relative to the AlAs-oxide aperture.
Gdf11 Facilitates Temporal Progression of Neurogenesis in the Developing Spinal Cord (2011)

Various types of neurons and glia are generated following a precise spatial and temporal order during neurogenesis. The mechanisms that control this sequential generation of neuronal and glial cell types from the same progenitor population are not well understood. Growth differentiation factor 11 (Gdf11) belongs to the TGF-β family of proteins and is expressed transiently in newly born neurons adjacent to the progenitor domain in the developing spinal cord. We examined the phenotypes of Gdf11(-/-) mouse embryos and found that without Gdf11, neuronal differentiation in the spinal cord progresses at a slower rate. Higher progenitor proliferation rate, along with a delay in gliogenesis, is also observed in Gdf11(-/-) spinal cord but only after the peak of Gdf11 expression, indicating that Gdf11 can cause long-lasting changes in progenitor properties. These changes can be preserved in vitro, as neurospheres derived from Gdf11(-/-) and wild-type littermates at a stage after, but not before the onset of Gdf11 expression, exhibit differences in proliferation and differentiation potential. Moreover, these changes in progenitor properties can be induced in vitro by the addition of Gdf11. We also demonstrate that the effects of Gdf11 on progenitor cells are associated with its ability to upregulate p57(Kip2) and p27(Kip1) while downregulating Pax6 expression. These results support a model in which Gdf11 secreted by newly born neurons in the developing spinal cord facilitates the temporal progression of neurogenesis by acting as a positive feedback signal on the progenitor cells to promote cell cycle exit and decrease proliferation ability, thus changing their differentiation potential.
Risperidone in the treatment of schizophrenia (1994)

Risperidone is a safe antipsychotic that is effective against both the positive and negative symptoms of schizophrenia.
Output of a Sound Source in a Reverberation Chamber and Other Reflecting Environments (1958)

It is important to know the output of a source as a function of position in a reverberation chamber. Expressions are given for the sound power output of simple monopole and dipole sources as functions of source position in various reflecting environments. They are obtained by the use of the method of images and a general formula due to Rayleigh for the output of a number of simple sources. The cases of a dipole source near a reflecting plane and a simple source near a reflecting edge and corner are treated, and the effect of the band width of the source is considered. The results apply when the reflectors enclose the source, as in a reverberation chamber, unless the distance in wavelengths between parallel walls is small and the absorption in the enclosure is low. Some experimental data are given, and the reverberation chamber method of measuring the power output of sources is discussed. In the general case of an extended source emitting nonspherical waves near reflecting surfaces, it may be more convenient to find the variation of power output with position by experiment than by calculation.
Platelet-compatible hydrophilic segmented polyurethanes from polyethylene glycols and cyclohexane diisocyanate. (1982)

A new type of segmented polyurethane (SPU) was synthesized from alpha, omega polyethylene oxide diols (PEG) of MW varying from 600 to 4500, by end capping with 1,4 trans cyclohexane diisocyanate (CHDI) and chain extending with ethylene diamine (ED) in toluene with dibutyl tin dilaurate as catalyst. These SPU are cast as films and coatings from hexafluroisopropanol (HFIP). Depending on PEG MW, these SPU swell two- to tenfold in water. Examined by an in vitro platelet retention test, these SPU are more bland (platelet retention -rho around 0.05) than most other polymers, whereas an alternating copolymer of CHDI and ED shows -rho around 0.80 (very active); x-ray photoelectron spectroscopy shows that the surfaces of these SPU to a depth of about 40 Ao are nearly pure PEO, unlike SPU synthesized from aromatic diisocyanates TDI and MDI.
Critical Residues in the Ligand-binding Site of the Staphylococcus aureus Collagen-binding Adhesin (MSCRAMM) (1995)

We have identified a discrete collagen-binding site within the Staphylococcus aureus collagen adhesin that is located in a region between amino acids Asp209 and Tyr233. Polyclonal antibodies raised against a recombinant form of the collagen adhesin inhibited the binding of collagen type II to S. aureus. When overlapping synthetic peptides mimicking segments of the adhesin fragment were tested for their ability to neutralize the inhibitory activity of the antibody only one peptide, CBD4 was found to be active. CBD4 bound directly to collagen and at high concentrations inhibited the binding of collagen to S. aureus. A synthetic peptide derivative of CBD4 lacking 2 carboxyl-terminal residues (Asn232, Tyr233) had no inhibitory activity. The importance of these residues for collagen binding was confirmed by biospecific interaction analysis. Mutant adhesin proteins N232-->A and Y233-->A exhibited dramatic changes in collagen binding activity. The dominant dissociation rate for the binding of mutant adhesin protein N232-->A to immobilized collagen II decreased almost 10-fold, while the Y233-->A and the double mutant exhibited even more significant decreases in affinity and apparent binding ratio when compared to the wild type protein.
Noninvasive determination of age-related changes in the human arterial pulse. (1989)

Arterial pressure waves were recorded noninvasively from the carotid, radial, femoral, or all three of these arteries of 1,005 normal subjects, aged 2-91 years, using a new transcutaneous tonometer containing a high fidelity Millar micromanometer. Waves were ensemble-averaged into age-decade groups. Characteristic changes were noted with increasing age. In all sites, pulse amplitude increased with advancing age (carotid, 91.3%; radial 67.5%; femoral, 50.1% from first to eighth decade), diastolic decay steepened, and diastolic waves became less prominent. In the carotid pulse, there was, in youth, a second peak on the downstroke of the waves in late systole. After the third decade, this second peak rose with age to merge with and dominate the initial rise. In the radial pulse, a late systolic wave was also apparent, but this occurred later; with age, this second peak rose but not above the initial rise in early systole, even at the eighth decade. In the femoral artery, there was a single systolic wave at all ages. Aging changes in the arterial pulse are explicable on the basis of both an increase in arterial stiffness with increased pulse-wave velocity and progressively earlier wave reflection. These two factors may be separated and effects of the latter measured from pressure wave-contour analysis using an "augmentation index," determined by a computer algorithm developed from invasive pressure and flow data. Changes in peak pressure in the central (carotid) artery show increasing cardiac afterload with increasing age in a normal population; this can account for the cardiac hypertrophy that occurs with advancing age (even as other organs atrophy) and the predisposition to cardiac failure in the elderly. Identification of mechanisms responsible offers a new approach to reduction of left ventricular afterload.
Expression of the CopB outer membrane protein by Moraxella catarrhalis is regulated by iron and affects iron acquisition from transferrin and lactoferrin (1996)

The amino acid sequence of the cell-surface-exposed, 81-kDa CopB outer membrane protein of Moraxella catarrhalis was found to be similar to those of TonB-dependent outer membrane proteins of other gram-negative bacteria. Expression of CopB was affected by the availability of iron in the growth medium, and the extent of overexpression of CopB in response to iron limitation varied widely among the M. catarrhalis strains tested. Wild-type M. catarrhalis strains were found to be able to utilize ferric citrate, transferrin, lactoferrin, and heme as sources of iron for growth in vitro. However, an isogenic copB mutant was severely impaired in its ability to utilize transferrin and lactoferrin as sole sources of iron for growth, whereas this same mutant grew similarly to the wild-type parent strain when supplied with ferric citrate as the iron source. The copB mutant was not significantly different from its wild-type parent strain in its ability to bind transferrin and lactoferrin. In addition, the wild-type parent strain and the copB mutant exhibited equivalent rates of uptake of 55Fe from ferric citrate. However, the copB mutant was markedly less able than the wild-type strain to take up 55Fe from transferrin and lactoferrin. These results indicate that lack of expression of the CopB protein exerts a direct or indirect effect on the ability of M. catarrhalis to utilize iron bound to certain carrier proteins.
Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells. (1982)

We have constructed a number of abbreviated dihydrofolate reductase (DHFR) genes by using cloned mouse genomic and cDNA sequences. These genes contain 1.0 kilobase of 5' flanking genomic sequence and varying portions of the 3' non-coding region. Two of the genes contain the first two introns of the DHFR gene; the other three lack introns. Transfection of DHFR-deficient Chinese hamster ovary cells with any of these constructed genes results in cells with the DHFR+ phenotype. Treatment of the transfectants with methotrexate, a folate antagonist, leads to the emergence of methotrexate-resistant colonies which have amplified the transfected genes.
ANTI‐HAIR KERATIN MONOCLONAL ANTIBODY (HKN‐2) (1985)

ABSTRACT BALB/c mice were immunized with human hair fibrous proteins. Then their spleen cells were fused with Sp2/0‐Agl4 mouse myeloma cells. Antibody‐producing hybridomas were selected by ELISA method and cloned by limiting dilution. A monoclonal antibody was chosen and designated as HKN‐2. Immunohistochemically, on frozen sections of normal human skin, HKN‐2 was found to recognize the suprabasal cells of epidermis and hair follicle, the cells in the keratogenous zone of hair shaft, the sebaceous cells and the ductal and myoepithelial cells of sweat glands. The basal cells of the epidermis and lower hair follicle, hair matrix cells and secretory cells of sweat glands did not react with HKN‐2. An immunoelectron microscopic method showed that the positive reaction to HKN‐2 was located on the tonofilaments in the cytoplasm. It was concluded that there might be a common antigenic determinant between hair and other skin epithelial tissues. A complexity in keratin manifestation in each epithelial tissue of the skin was suspected.
The Ability of Herpes Simplex Virus Type 1 Immediate-Early Protein Vmw110 To Bind to a Ubiquitin-Specific Protease Contributes to Its Roles in the Activation of Gene Expression and Stimulation of Virus Replication (1999)

Herpes simplex virus type 1 immediate-early protein Vmw110 stimulates the onset of virus infection and is required for efficient reactivation from latency. In transfection assays, Vmw110 is a potent activator of gene expression, but its mode of action has yet to be determined. Previous work has shown that Vmw110 localizes to specific intranuclear structures known as ND10, PML bodies, or PODs and causes the disruption of these domains. The ability of Vmw110 to disrupt ND10 correlates with its biological activities in infected and transfected cells. It has also been found that Vmw110 binds strongly and specifically to a ubiquitin-specific protease known as HAUSP, itself a component of a subset of ND10. In this study we have investigated the role of HAUSP in Vmw110 activity; single amino acid residues of Vmw110 required for the interaction were identified, and the effects of mutation of these residues in infected and transfected cells were then assayed. The results indicate that the ability to bind to HAUSP contributes to the functional activities of Vmw110.
A Neural Network Controller for Continuum Robots (2007)

Continuum or hyper-redundant robot manipulators can exhibit behavior similar to biological trunks, tentacles, or snakes. Unlike traditional rigid-link robot manipulators, continuum robot manipulators do not have rigid joints, hence these manipulators are extremely dexterous, compliant, and are capable of dynamic adaptive manipulation in unstructured environments. However, the development of high-performance control algorithms for these manipulators is quite a challenge, due to their unique design and the high degree of uncertainty in their dynamic models. In this paper, a controller for continuum robots, which utilizes a neural network feedforward component to compensate for dynamic uncertainties is presented. Experimental results using the OCTARM, which is a soft extensible continuum manipulator, are provided to illustrate that the addition of the neural network feedforward component to the controller provides improved performance.
Recent Advances in the Synthesis of Antibacterial Quinolones (1992)

This review surveys the main synthetic approaches used for the construction of antibacterial quinolones, including aza analogs (naphthyridones, cinnolones) and condensed polycyclic analogs, with an emphasis on the more versatile methods which are potentially useful in other fields of heterocyclic chemistry.Simpler non-condensed monocyclic analogs, i.e., pyridone and pyridazinone carboxylic acids are also briefly mentioned.
Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory Syndrome (2003)

In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of SARS-CoV is 29,727 nucleotides in length and has 11 open reading frames, and its genome organization is similar to that of other coronaviruses. Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closely related to any of the previously characterized coronaviruses.
Potential of a suite of robot/computer-assisted motivating systems for personalized, home-based, stroke rehabilitation (2007)

The Robot/CAMR suite has potential for stroke rehabilitation. By manipulating hardware and software variables, we can create personalized therapy environments that engage patients, address their therapy need, and track their progress. A larger longitudinal study is still needed to evaluate these systems in under-supervised environments such as the home.
Rho, rac and cdc42 GTPases: regulators of actin structures, cell adhesion and motility (1995)

Conference Article| August 01 1995 Rho, rac and cdc42 GTPases: regulators of actin structures, cell adhesion and motility C. D. Nobes; C. D. Nobes 1CRC Oncogene and Signal Transduction Group, MRC Laboratory for Molecular Cell Biology and Department of Biochemistry, University College London, London WC1E 6BT, U.K. Search for other works by this author on: This Site PubMed Google Scholar A. Hall A. Hall 1CRC Oncogene and Signal Transduction Group, MRC Laboratory for Molecular Cell Biology and Department of Biochemistry, University College London, London WC1E 6BT, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1995) 23 (3): 456–459. https://doi.org/10.1042/bst0230456 Article history Received: March 02 1995 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation C. D. Nobes, A. Hall; Rho, rac and cdc42 GTPases: regulators of actin structures, cell adhesion and motility. Biochem Soc Trans 1 August 1995; 23 (3): 456–459. doi: https://doi.org/10.1042/bst0230456 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: LPA, lysophosphatidic acid, PDGF, platelet-derived growth factor, PI 3-kinase, phosphatidyl-inositol 3-kinase © 1995 Biochemical Society1995 Article PDF first page preview Close Modal You do not currently have access to this content.
Genetics and prospective therapeutic targets for Sjögren-Larsson Syndrome (2016)

The most promising pharmacologic approach to SLS involves blocking the formation of potentially harmful fatty aldehyde adducts using aldehyde scavenging drugs, currently in phase 2 clinical trials. Other approaches needing further investigation include: 1) ALDH-specific activator drugs and PPAR-α agonists to increase mutant FALDH activity; 2) inhibitors of the JNK phosphorylation cascade; 3) antioxidants to decrease aldehyde load; 4) dietary lipid modification; and 5) gene therapy.
Properties of the yeast nuclear histone deacetylase (1994)

A nuclear histone deacetylase from yeast was partially purified and some of its characteristics were studied. Histone deacetylase activity was stimulated in vitro by high-mobility-group nonhistone chromatin proteins 1 and 2 and ubiquitin and inhibited by spermine and spermidine, whereas n-butyrate had no significant inhibitory effect. Like the mammalian enzyme, partially purified histone deacetylase from yeast was strongly inhibited by trichostatin A. However, in crude extract preparations the yeast enzyme was not inhibited and treatment with trichostatin in vivo did not show any effect, either on the histone acetylation level or on cell viability. At low ionic strength, the enzyme can be isolated as a complex of high molecular mass that is much less inhibited by trichostatin A than is partially purified histone deacetylase activity. Furthermore, radiolabelled oligonucleosomes were more efficiently deacetylated by the complex than by the low-molecular-mass form of the enzyme. The histone deacetylase activity was separated from a polyamine deacetylase activity and its specificity studied. Using h.p.l.c.-purified core histone species as substrate, histone deacetylase from yeast is able to deacetylate all core histones with a slight preference for H3. Our results support the idea that the yeast histone deacetylase may act as a high-molecular-mass complex in vivo.
An optically pumped silicon evanescent laser operating continuous wave at 60/spl deg/C (2006)

We demonstrate a silicon evanescent laser operating CW up to 60/spl deg/C. The laser operates at 1568 nm with a maximum fiber-coupled output power of 4.5 mW. Devices are fabricated with silicon waveguides bonded to AlGaInAs quantum wells.
Organic Thin Film Transistors for Large Area Electronics (2002)

Organic thin-film transistors (OTFTs) have lived to see great improvements in recent years. This review presents new insight into conduction mechanisms and performance characteristics, as well as opportunities for modeling properties of OTFTs. The shifted focus in research from novel chemical structures to fabrication technologies that optimize morphology and structural order is underscored by chapters on vacuum-deposited and solution-processed organic semiconducting films. Finally, progress in the growing field of the n-type OTFTs is discussed in ample detail. The Figure, showing a pentacene film edge on SiO2, illustrates the morphology issue.
A Facile Synthesis and Physical Properties of Nano-Sized Dendritic <I>α</I>,<I>ε</I>-Poly(L-lysine)s for the Delivery of Nucleic Acids (2005)

A series of nano-sized dendritic alpha,epsilon-poly(L-lysine)s (DPL) were synthesized by the solid-phase peptide synthesis method, using a core epsilon-peptide structure consisting of eight lysine residues. Surface amines of dendritic alpha,epsilon-poly(L-lysine) were characterized by comparing the retention times of a reverse phase HPLC with the electrophoretic mobilities of capillary zone electrophoresis (CZE) and non-denatured polyacrylamide gel electrophoresis (PAGE). The elution times of alpha,epsilon-poly(Llysine) in HPLC were well correlated with the electrophoretic mobilities of CZE and PAGE. The separation was dependent on size, shape of molecule and the number of surface amine. The alpha,epsilon-poly(L-lysine) formed a complex with nucleic acids at various charge ratios and the degree of complex formation was size- and structure-dependent. Atomic force microscopy of the complex visualized the size and morphology of alpha,epsilon-poly(L-lysine)/DNA complex as a nano-sized spherical shape. The small size in complex formation provides a promise for in vivo therapeutic application of dendritic alpha,epsilon-poly(L-lysine)s or their derivatives in the delivery of gene or oligonucleotide.