gebhart/patrepeval huggingface dataset:
Transferrin–Liposome-Mediated p53 Sensitization of Squamous Cell Carcinoma of the Head and Neck to Radiation In Vitro (1997)

Wild-type (wt) p53 DNA was transfected into the radioresistant human cell line JSQ-3, established from a squamous cell carcinoma of the head and neck (SCCHN), using a transferrin-liposome system, and the ability of the introduced wt p53 to sensitize the transfected JSQ-3 cells to ionizing radiation was examined. Transferrin increased the in vitro transfection efficiency of cationic liposomes up to 70-80% in JSQ-3 cells, representing a 6- to 10-fold increase over liposome transfection alone. The exogenous wt p53 was expressed at high levels in transferrin-liposome-DNA-transfected cells and resulted in the reversion of the radioresistant phenotype of the JSQ-3 cells in a DNA dose-dependent manner. The D10 values were reduced from 6.36 +/- 0.54 Gy to 4.13 +/- 0.06 Gy, a value in the radiosensitive range. In vivo, the intratumoral injection of the transferrin-liposome system resulted in a higher number of transfected tumor cells in the JSQ-3 induced nude mouse xenografts when compared with transfection by liposome alone. The results indicate that the combination of p53 replacement gene transduction, mediated by the relatively safe transferrin-liposome system, and conventional ionizing radiation may provide a more effective treatment for head and neck cancer.
Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay (2006)

RNA aptamers that bind the opium alkaloid codeine were generated using an iterative in vitro selection process. The binding properties of these aptamers, including equilibrium and kinetic rate constants, were determined through a rapid, high-throughput approach using surface plasmon resonance (SPR) analysis to measure real-time binding. The approach involves direct coupling of the target small molecule onto a sensor chip without utilization of a carrier protein. Two highest binding aptamer sequences, FC5 and FC45 with K(d) values of 2.50 and 4.00 microM, respectively, were extensively studied. Corresponding mini-aptamers for FC5 and FC45 were subsequently identified through the described direct coupling Biacore assays. These assays were also employed to confirm the proposed secondary structures of the mini-aptamers. Both aptamers exhibit high specificity to codeine over morphine, which differs from codeine by a methyl group. Finally, the direct coupling method was demonstrated to eliminate potential non-specific interactions that may be associated with indirect coupling methods in which protein linkers are commonly employed. Therefore, in addition to presenting the first RNA aptamers to a subclass of benzylisoquinoline alkaloid molecules, this work highlights a method for characterizing small molecule aptamers that is more robust, precise, rapid and high-throughput than other commonly employed techniques.
Dynamiek en patronen in interactie en onderhandeling: waarden van natuur en landschap (2003)

Meningen en opvattingen van agrariers over natuur en natuurbeleid zijn geanalyseerd, waarna het fenomeen natuurbeelden aan de orde komt en wat daaraan ten grondslag ligt. Vervolgens worden de interacties en onderhandelingen over het beheer van natuur en landschap tussen verschillende belanghebbenden nader geduid. Tenslotte presenteert de auteur een ander perspectief op planning en beleid rond natuur- en landschapsbeheer
A constrained optimization approach to virtual fixtures for multi-handed tasks (2008)

In this work, we have extended the concept of constrained motion control of robots to surgical tasks that require multiple robots. We present virtual fixtures to guide the motion of multiple robots such that spatial and temporal relationship is maintained between them. At the same time, our algorithm keeps the surgeon in the control loop. Moreover, we show that these virtual fixtures allow bimanual tasks to be completed using input for a single robot. That is, the user requires only one hand to cooperatively control multiple robots. This reduces the cognitive load on the surgeon and makes multiple-robot setup for surgery more relevant. We demonstrate this architecture by using an example of manipulating a surgical knot to position it at a target point. Significant improvement is observed in the accuracy when bimanual virtual fixture assistance is provided. Moreover, the accuracy when using a single input from user is similar to the accuracy obtained from bimanual assistance.
Peptides Derived from Type IV Collagen, CXC Chemokines, and Thrombospondin-1 Domain-Containing Proteins Inhibit Neovascularization and Suppress Tumor Growth in MDA-MB-231 Breast Cancer Xenografts (2009)

Angiogenesis or neovascularization, the process of new blood vessel formation from preexisting microvasculature, involves interactions among several cell types including parenchymal, endothelial cells, and immune cells. The formation of new vessels is tightly regulated by a balance between endogenous proangiogenic and antiangiogenic factors to maintain homeostasis in tissue; tumor progression and metastasis in breast cancer have been shown to be angiogenesis-dependent. We previously introduced a systematic methodology to identify putative endogenous antiangiogenic peptides and validated these predictions in vitro in human umbilical vein endothelial cell proliferation and migration assays. These peptides are derived from several protein families including type IV collagen, CXC chemokines, and thrombospondin-1 domain-containing proteins. On the basis of the results from the in vitro screening, we have evaluated the ability of one peptide selected from each family named pentastatin-1, chemokinostatin-1, and properdistatin, respectively, to suppress angiogenesis in an MDA-MB-231 human breast cancer orthotopic xenograft model in severe combined immunodeficient mice. Peptides were administered intraperitoneally once per day. We have demonstrated significant suppression of tumor growth in vivo and subsequent reductions in microvascular density, indicating the potential of these peptides as therapeutic agents for breast cancer.
Identification of the RPE65 protein in mammalian cone photoreceptors. (2002)

RPE65 is expressed in mammalian cones, but not in rods. These results provide further support for physiological observations that cones may have an alternative retinoid cycle.
The Chemistry of amidines and imidates (1975)

General and theoretical aspects, G.Hafelinger structural chemistry, T.M.Krygowski and K.Wozniak stereochemical aspects of amidines, imidates and related derivatives, C.L.Perrin analysis, J.Oszczapowicz and I.Oszczapowicz mass spectra, S.Fornarini thermochemistry, K.Pihiaja synthesis of amidines, G.Boyd synthetic uses of amidines and related compounds, G.Boyd imidates, D.G.Nielson synthesis, chemistry and uses of guanidine derivatives, K.Akiba rearrangements, V.Minkin and I.E.Mikhailov tautomerism, H-bonding and basicity, J.Oszczapowicz directing and activating effects, J.Shorter biochemistry, pharmacology and toxicology of amidines and related compounds, R.J.Grout isotopically substituted amidines, P.J.Smith photochemistry and radiation chemistry of amidines and related compounds, Z.B.Alfassi catalysis by amidines, P.N.Ahlberg electrochemistry, J.Jaworski and M.Kalinowski.
Safety assessment for pharmaceuticals (1995)

Strategy and Phasing for Drug Safety Evaluation in the Discovery and Development of Pharmaceuticals - S. Gad Information Sources: Building and Maintaining Data Files - D. Larson, et al. Screens in Safety and Hazard Assessments - S. Gad Acute Toxicity Testing in Drug Safety Evaluation - C. Chengelis Subchronic and Chronic Toxicity Studies - S. Gad Developmental and Reporductive Toxicity Testing - R. Clark Carcinogenicity Studies - L. DePass Safety Assessment of Inhalant Drugs - B. Leong Irritation and Local Tissue Tolerance in Pharmaceutical Safety Assessment - S. Gad Routes, Formulations, and Dose Designs - S. Gad Occupational Toxicology in the Pharmaceutical Industry - D. Galer Immunotoxicology in Pharmaceutical Development - P. Guzzie Special Case: Nonrodent Studies - G. Haggerty The Application of In Vitro Techniques in Drug Safety Assessment - P. Williams, et al. Appendix Index.
Peptide Vaccines for Cancer (2002)

The revelation that immune cytolytic and helper T-cells recognize intracellularly degraded peptides processed via the proteosome apparatus, inserted into the endoplasmic reticulum and transported to the surface for association with major histocompatibility locus (MHC) molecules on specialized antigen-presenting cells has revolutionized the cancer vaccine field. Understanding the science of antigen processing and presentation has provided new reagents, delivery systems, and new investigative leads for the generation of immune responses against cancer. The cloning of tumor antigen genes has proceeded rapidly in melanoma, due to the ease with which melanoma-specific T-cells can be propagated in vitro, but breast, cervix, and lung cancer are not far behind. The cloning and identification of tumor antigens recognized by T-cells and data from initial clinical trials with peptides vaccines derived from those antigens are presented.
Breast cancer signatures for invasiveness and prognosis defined by deep sequencing of microRNA (2012)

The transition from ductal carcinoma in situ to invasive ductal carcinoma is a key event in breast cancer progression that is still not well understood. To discover the microRNAs regulating this critical transition, we used 80 biopsies from invasive ductal carcinoma, 8 from ductal carcinoma in situ, and 6 from normal breast. We selected them from a recently published deep-sequencing dataset [Farazi TA, et al. (2011) Cancer Res 71:4443-4453]. The microRNA profile established for the normal breast to ductal carcinoma in situ transition was largely maintained in the in situ to invasive ductal carcinoma transition. Nevertheless, a nine-microRNA signature was identified that differentiated invasive from in situ carcinoma. Specifically, let-7d, miR-210, and -221 were down-regulated in the in situ and up-regulated in the invasive transition, thus featuring an expression reversal along the cancer progression path. Additionally, we identified microRNAs for overall survival and time to metastasis. Five noncoding genes were associated with both prognostic signatures--miR-210, -21, -106b*, -197, and let-7i, with miR-210 the only one also involved in the invasive transition. To pinpoint critical cellular functions affected in the invasive transition, we identified the protein coding genes with inversely related profiles to miR-210: BRCA1, FANCD, FANCF, PARP1, E-cadherin, and Rb1 were all activated in the in situ and down-regulated in the invasive carcinoma. Additionally, we detected differential splicing isoforms with special features, including a truncated EGFR lacking the kinase domain and overexpressed only in ductal carcinoma in situ.
Intradose and Circadian Variation in Circulating Carbamazepine and Its Epoxide in Epileptic Patients: A Consequence of Autoinduction of Metabolism (1987)

Total and free carbamazepine (CBZ), and CBZ 10,11 epoxide (CBZ-E) concentrations were measured over 24 h in 19 patients receiving CBZ 400 mg b.i.d. either as monotherapy (n = 13) or combined with another anticonvulsant (n = 6). Differences in CBZ and CBZ-E disposition between day and night dosing were minor. Mean plasma CBZ concentrations were higher and CBZ-E/CBZ ratios were lower in the monotherapy patients. Variations in total and free plasma CBZ levels were comparable in the monotherapy and polypharmacy groups. Peak free and total CBZ concentrations coincided at approximately 4 h postdose. Free CBZ levels correlated significantly with total in each patient. The extent of variation in total plasma CBZ concentration during 24 h correlated significantly with antipyrine clearance in the monotherapy group. Circadian rhythms are unlikely to influence CBZ disposition to a clinically relevant extent. Measurement of peak and trough CBZ concentrations should improve the value of therapeutic drug monitoring. The diurnal variation in CBZ concentration appears related to the degree of autoinduction of metabolism and is substantial enough to warrant the development of a slow-release preparation of the drug.
<title>Locating facial features for age classification</title> (1993)

In this paper, we outline computations for visual age classification from facial images. For now, input images can only be classified into one of three age-groups: babies, adults, and senior adults. The computations are based on cranio-facial development theory, and wrinkle analysis. In the implementation, first primary features of the face are found, followed by secondary feature analyses. Preliminary results with real data are presented.
Heuristic Scheduling Algorithms for Allocation of Virtualized Network and Computing Resources (2013)

Cloud computing technology facilitates computing-intensive applications by providing virtualized resources which can be dynamically provisioned. However, user’s requests are varied according to different applications’ computation ability needs. These applications can be presented as meta-job of user’s demand. The total processing time of these jobs may need data transmission time over the Internet as well as the completed time of jobs to execute on the virtual machine must be taken into account. In this paper, we presented V-heuristics scheduling algorithm for allocation of virtualized network and computing resources under user’s constraint which applied into a service-oriented resource broker for jobs scheduling. This scheduling algorithm takes into account both data transmission time and computation time that related to virtualized network and virtual machine. The simulation results are compared with three different types of heuristic algorithms under conventional network or virtual network conditions such as MCT, Min-Min and Max-Min. e evaluate these algorithms within a simulated cloud environment via an abilenenetwork topology which is real physical core network topology. These experimental results show that V-heuristic scheduling algorithm achieved significant performance gain for a variety of applications in terms of load balance, Makespan, average resource utilization and total processing time.
Recovery Techniques for Database Systems (1978)

article Recovery Techniques for Database Systems Share on Author: Joost S. M. Verhofstad BNSR, 522 University Avenue, Toronto, Ontario M5G 1W7 Canada BNSR, 522 University Avenue, Toronto, Ontario M5G 1W7 CanadaView Profile Authors Info & Claims ACM Computing SurveysVolume 10Issue 2June 1978 pp 167–195https://doi.org/10.1145/356725.356730Online:01 June 1978Publication History 130citation2,559DownloadsMetricsTotal Citations130Total Downloads2,559Last 12 Months39Last 6 weeks4 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Expression and Function of Surface Antigens on Scleroderma Fibroblasts (1991)

Dermal fibroblasts from patients with systemic sclerosis (SSc) bound a much greater number of T lymphocytes than did normal dermal fibroblasts. Monoclonal antibodies (MAb) against classes I and II antigens of the major histocompatibility complex (MHC) and their receptors, CD8 and CD4, had no effect on T cell interaction with SSc and normal cells, while MAb against lymphocyte function-associated antigen type 3 (LFA-3) and CD2 both strongly inhibited lymphocyte attachment. MAb against intercellular adhesion molecule type 1 (ICAM-1) and LFA-1 also prevented binding of T lymphocytes, but had a more marked effect on adhesion to SSc fibroblasts than to normal fibroblasts; they also completely abolished the increased binding to fibroblasts treated with interleukin-1 alpha, tumor necrosis factor alpha, and interferon-gamma. No difference was found in the proportion of normal and SSc fibroblasts that expressed MHC classes I and II and LFA-3, but more SSc cells expressed ICAM-1, and at a higher level, than did normal fibroblasts. These results show that cultured SSc cells have elevated binding to T lymphocytes, which possibly results from expansion of a subset of fibroblasts that produces high levels of ICAM-1.
A Drought Resistance-Promoting Microbiome Is Selected by Root System under Desert Farming (2012)

Crop cultivation provides critical ecosystem services in arid lands with the plant root system acting as a "resource island" able to attract and select microbial communities endowed with multiple PGP traits that sustain plant development under water limiting conditions.
Ability of bacteria associated with chronic inflammatory disease to stimulate E-selectin expression and promote neutrophil adhesion (1995)

Porphyromonas gingivalis, Pseudomonas aeruginosa, and Helicobacter pylori have been shown to be associated with adult periodontal disease, chronic lung infections, and peptic ulcers, respectively. The ability of these bacteria to stimulate E-selectin expression and promote neutrophil adhesion, two components necessary for the recruitment of leukocytes in response to infection, was investigated. Little or no stimulation of E-selectin expression was observed with either P. gingivalis or H. pylori when whole cells, lipopolysaccharide (LPS), or cell wall preparations added to human umbilical cord vein endothelial cells were examined. P. aeruginosa was able to induce E-selectin to near-maximal levels; however, it required approximately 100 to 1,000 times more whole cells or LPS than that required by E. coli. Neutrophil-binding assays revealed that LPS and cell wall preparations obtained from these bacteria did not promote endothelial cell adhesiveness by E-selectin-independent mechanisms. In addition, P. gingivalis LPS blocked E-selectin expression by LPS obtained from other bacteria. We propose that lack of E-selectin stimulation and the inability to promote endothelial cell adhesiveness are two additional indications of low biologically reactive LPS. We suggest that this property of LPS may contribute to host tissue colonization. In addition, the ability of P. gingivalis to inhibit E-selectin expression may represent a new virulence factor for this organism.
Proteasomal Degradation of Mutant Superoxide Dismutases Linked to Amyotrophic Lateral Sclerosis (2005)

Mutations in copper-zinc superoxide dismutase cause the neurodegenerative disease amyotrophic lateral sclerosis. Many of the mutant proteins have increased turnover in vivo and decreased thermal stability. Here we show that purified, metal-free superoxide dismutases are degraded in vitro by purified 20 S proteasome in the absence of ATP and without ubiquitinylation, whereas their metal-bound counterparts are not. The rate of degradation by the proteasome varied among the mutants studied, and the rate correlated with the in vivo half-life. The monomeric forms of both mutant and wild-type superoxide dismutase are particularly susceptible to degradation by the proteasome. Exposure of hydrophobic regions as a consequence of decreased thermal stability may allow the proteasome to recognize these molecules as non-native.
Single insertion of .alpha.-olefins into the cationic complex [Zr(CH2Ph)3]+ affording isolable [Zr(CH2Ph)2(CH2CHRCH2Ph)]+ adducts: a model for the insertion mechanism in Ziegler-Natta polymerization (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSingle insertion of .alpha.-olefins into the cationic complex [Zr(CH2Ph)3]+ affording isolable [Zr(CH2Ph)2(CH2CHRCH2Ph)]+ adducts: a model for the insertion mechanism in Ziegler-Natta polymerizationClaudio Pellecchia, Alfonso Grassi, and Adolfo ZambelliCite this: Organometallics 1994, 13, 1, 298–302Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/om00013a043https://doi.org/10.1021/om00013a043research-articleACS PublicationsRequest reuse permissionsArticle Views195Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Molecular Engineering of Viral Gene Delivery Vehicles (2008)

Viruses can be engineered to efficiently deliver exogenous genes, but their natural gene delivery properties often fail to meet human therapeutic needs. Therefore, engineering viral vectors with new properties, including enhanced targeting abilities and resistance to immune responses, is a growing area of research. This review discusses protein engineering approaches to generate viral vectors with novel gene delivery capabilities. Rational design of viral vectors has yielded successful advances in vitro, and to an extent in vivo. However, there is often insufficient knowledge of viral structure-function relationships to reengineer existing functions or create new capabilities, such as virus-cell interactions, whose molecular basis is distributed throughout the primary sequence of the viral proteins. Therefore, high-throughput library and directed evolution methods offer alternative approaches to engineer viral vectors with desired properties. Parallel and integrated efforts in rational and library-based design promise to aid the translation of engineered viral vectors toward the clinic.