gebhart/patrepeval huggingface dataset:
Impact of power quality sensor technique on power system protection voltage transient measurements (2010)

With the increasing connections of generation using power electronics and transmission network capacity using embedded FACTS devices, the measurement of higher than 50th voltage harmonics is of growing importance. This paper presents how to use the recent proposed Power Quality Sensor (PQ Sensor) adding onto the existing Capacitor Voltage Transformer (CVT) infrastructure to measure high orders of voltage harmonics and voltage transients accurately. A CVT circuit with the PQ sensor was modelled and simulated. The PQ Sensor performances were evaluated and tested under various CVT burden conditions. The results show that the PQ sensor has excellent linear frequency response, in terms of magnitude and phase, over the studied frequency range from 10 Hz to 10 kHz. The sensitivity studies show that the PQSensor frequency response performances are only affected the change of the capacitors of the CVT, and not the burden of the CVT. (4 pages)
Conserved sequences of the adenovirus genome for detection of all human adenovirus types by hybridization (1992)

The application of DNA hybridization directly to clinical specimens has the potential of improving the diagnosis of fastidious types of adenovirus. In this study, the genome of one adenovirus type from each human subgenus (A to F) was systematically evaluated by hybridization for homologous sequences to find the optimal common probe for detection of all human adenovirus types. The area of cross-hybridization, most closely defined with adenovirus type 2 (Ad2), mapped from map units 11.4 to 16.1 and 26.9 to 29.7 and, principally, to a central area of the genome between map units 47.5 and 65.2. The last area, enclosing the hexon gene, was highly conserved. Cloned probes generated from this area demonstrated the greatest homology to heterologous types by hybridization analysis. A HindIII-BglII clone containing the hexon gene of Ad2 within narrow confines reacted most evenly with all adenoviral types and detected the DNA of all other subgenera with a sensitivity 2 logs greater than that of a complete genomic Ad2 probe. The most homologous adenoviral gene sequences were observed in genes involved with DNA replication or intimately connected to the hexon in the early capsid formation. These results show that the hexon gene constitutes the best single region of the adenovirus genome for use as a genus-specific probe for the diagnosis of all human adenoviral subgenera by DNA hybridization.
The preparation and application of functionalised synthetic oligonucleotides: III. Use of H-phosphonate derivatives of protected amino-hexanol and mercapto-propanol or-hexanol (1988)

Syntheses of H-phosphonate salts (4a-e) of N/S-protected alcohols such as 6-aminohexan-1-ol, 3-mercaptopropan-1-ol and 6-mercaptohexan-1-ol are described using 2-chloro-5,6-benzo-1,3,2-phosphorin-4-one (2) as the phosphonylating agent. The H-phosphonate salts (4a-e), in the presence of pivaloyl chloride or adamantoyl chloride as an activator, were coupled to the 5'-end of synthetic oligonucleotides on solid supports to produce amino or thio-linked oligonucleotides. Following deprotection and purification, fluorescent dyes, biotin derivatives and poly-L-lysine-maleimide were separately attached to the functionalised oligonucleotides. Identical derivatized oligomers were obtained with cyanoethyl-N,N-diisopropylamidite chemistry and amidites (5a-e) of the respective alcohols.
InterNote: extending a hypermedia framework to support annotative collaboration (1989)

Based on three years of user feedback, a design team at IRIS embarked on a project to enhance Intermedia to better support small groups of collaborators, particularly those involved with document review and revision. Towards this end, we defined user-level requirements for the new functionality. The result of this process was the design and implementation of InterNote. One aspect of InterNote involves a fundamental extension to Intermedia's navigational linking paradigm. Instead of simply being able to traverse links, users are now also able to transfer data across the links using a technique we call warm linking. In this paper we describe extensions to our hypermedia framework to support annotative collaboration, including the user interface of the new linking functionality and the InterNote extension. Finally, we discuss our plans for future work.
Molecular Epidemiology of Glanders, Pakistan (2009)

We collected epidemiologic and molecular data from Burkholderia mallei isolates from equines in Punjab, Pakistan from 1999 through 2007. We show that recent outbreaks are genetically distinct from available whole genome sequences and that these genotypes are persistent and ubiquitous in Punjab, probably due to human-mediated movement of equines.
The morphology and structure of intercalated and pillared clays (2008)

This thesis is submitted in a format of published papers by the candidate. Advanced methods of electron microscopy and X-ray spectroscopy have been used to study the relationship between the pillars and the silicate structure ranging from Al13 and Ga13 complexes to the final products Al- and Ga-pillared clays. The Al13 and Ga13 pillared montmorillonites have been prepared by conventional and ultrasonic methods. The ultrasonic method has been proven to be effective and showed very good catalytically activity. Transmission electron microscopy combined with elemental mapping by EDS showed the distribution of the Ga and Al pillars in the clay structure. The use of gallium allowed the independent observation of the Ga pillar distribution from the Al distribution in the clay structure.
XPS spectra of the Ga13 pillared montmorillonites showed that the pillars has been changed from the original Keggin structure with a 7+ charge to something more stable with a lower charge upon intercalation. No direct evidence of the inverted silicon tetrahedron structure bonding to the pillar structure, as suggested by Plee in his original thesis, was observed. For comparative reasons the major aluminium hydroxide minerals in bauxite (gibbsite, bayerite and (pseudo-) boehmite) were studied. 
Detailed information about the Al13 structure was obtained by studying the basic sulphate and nitrate salts with XPS. The XPS results of a set of starting clays in comparison to the pillared clays indicated that small changes in the binding energy could explain the changes in the pillar structure and the formation of chemical bonds to the clay tetrahedral sheets during the calcination leading to the final products.
Disposable potentiometric enzyme sensor for direct determination of organophosphorus insecticides (2000)

A potentiometric disposable enzyme sensor for the direct and fast determination of organophosphorus (OP) insecticides was developed by using an organophosphorus hydrolase (OPH) immobilized on an ion-selective electrode. The disposable screen-printed transducer was based on double matrix membrane technology which allows easy mass production. The potentiometric device consisted of a H(+)-sensitive electrode with integrated Ag/AgCl reference electrode. The electrodes were prepared with N,N-dioctadecylmethylamine as H(+)-sensitive ionophore and pH calibration resulted in slopes of 55 mV decade-1 over a pH range from 11 to 6. OPH was isolated from recombinant Escherichia coli DH5 alpha and immobilized within poly(carbamoyl sulfonate) prepolymer on the surface of the H(+)-sensitive electrode without any further fixation membrane. OPH catalyzes the hydrolytic cleavage of OP compounds which releases protons in a concentration proportional to hydrolyzed substrate. Sensor performance was investigated with regard to enzyme load, concentration, pH and temperature of the measuring buffer using paraoxon as analyte. Best sensitivity and response time were obtained with sensors prepared with 250 U of OPH and measuring at 37 degrees C in 1.0 mM HEPES buffer, pH 9.3, containing 100 mM NaCl. The enzyme sensor exhibited a linear calibration range of 0.01-0.15 mM chlorpyrifos, 0.05-0.35 mM diazinon, 0.05-0.4 mM paraoxon and 0.007-0.05 mM parathion, respectively. For all these analytes response times to reach 95% of maximum change in potential did not exceed 5 min. Sensors stored under dry conditions at 4 degrees C still showed 60% of initial hydrolytic rate after 70 d. The sensors even when stored dry were ready for measurements after 5 min incubation in measuring buffer. A range of putative interfering substances did not influence sensor response, and suitability of measuring OPs in soil extracts was ascertained.
Haploid induction and its genetic mechanism in alloplasmic common wheat (1980)

Journal Article Haploid induction and its genetic mechanism in alloplasmic common wheat Get access MITSUE KOBAYASHI, MITSUE KOBAYASHI graduate student Laboratory of Genetics, Faculty of Agriculture, Kyoto UniversitySakyo-ku, Kyoto, Japan 606 Search for other works by this author on: Oxford Academic PubMed Google Scholar KOICHIRO TSUNEWAKI KOICHIRO TSUNEWAKI professor Laboratory of Genetics, Faculty of Agriculture, Kyoto UniversitySakyo-ku, Kyoto, Japan 606 Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Heredity, Volume 71, Issue 1, January 1980, Pages 9–14, https://doi.org/10.1093/oxfordjournals.jhered.a109317 Published: 01 January 1980
Genetically directed syntheses of new polymeric materials: efficient expression of a monodisperse copolypeptide containing fourteen tandemly repeated -(AlaGly)4ProGluGly- elements (1991)

The statistical nature of chemical polymerization processes ensures that the materials prepared by such methods will consist of mixtures of chains. In contrast, ribosomal catalysis of protein synthesis on messenger RNA templates affords homogeneous products in which chain length, sequence, and stereochemistry are subject to exquisite control. With the advent of recombinant DNA methods in the 1970s, it has become possible to exploit this control in a synthetic sense, Le., to create new, artificial polypeptides of predetermined structure. Given the diversity of functionality, sequence, conformation, and chain lengths that become accessible in this way, it appears certain that this approach will find important applications in polymer materials science.
The Anticariogenic Effect of Sugar-Free Gum Containing CPP-ACP Nanocomplexes on Approximal Caries Determined Using Digital Bitewing Radiography (2008)

This study investigated, using digital bitewing radiography, the progression and regression of approximal caries in adolescent subjects chewing a sugar-free gum containing 54 mg CPP-ACP relative to the identical gum without CPP-ACP. 2,720 subjects from 29 schools were randomly assigned to one of the two gums and were instructed to chew their assigned gum for 3 x 10 min/day, with one session supervised on school days, over the 24-month study period. Standardized digital bitewing radiographs were taken at the baseline and 24-month clinical examinations for each subject. The radiographs, scored by a single examiner, were assessed for approximal surface dental caries at both the enamel and dentine level. Surface level transitions were scored using a transition matrix. Caries progression or regression was analysed using proportional-odds ordered logistic regression modelling of the transition scores at the tooth surface level. There was a statistically significant difference in the frequency distributions of the transition scores between the two gum groups (OR = 0.82, p = 0.03). For subjects chewing the CPP-ACP gum the odds of a surface experiencing caries progression were 18% less than those of a surface experiencing caries progression for subjects chewing the control gum. In conclusion, the 54 mg CPP-ACP sugar-free gum significantly slowed progression and enhanced regression of approximal caries relative to a control sugar-free gum in a 24-month clinical trial.
Archaebacterial DNA-dependent RNA polymerases testify to the evolution of the eukaryotic nuclear genome. (1989)

Genes for DNA-dependent RNA polymerase components B, A, and C from the archaebacterium Sulfolobus acidocaldarius and for components B", B', A, and C from the archaebacterium Halobacterium halobium were cloned and sequenced. They are organized in gene clusters in the order above, which corresponds to the order of the homologous rpoB and rpoC genes in the corresponding operon of the Escherichia coli genome. Derived amino acid sequences of archaebacterial components A and C were aligned with each other and with the sequences of corresponding (largest) subunits from the archaebacterium Methanobacterium thermoautotrophicum, with sequences of various eukaryotic nuclear RNA polymerases I, II, and III, and with the sequence of the beta' component from E. coli polymerase. The archaebacterial genes for component A are homologous to about the first two-thirds of genes for the eukaryotic component A and the eubacterial component beta', and the archaebacterial genes for component C are homologous to the last third of the genes for the eukaryotic component A and the eubacterial component beta'. Unrooted phylogenetic dendrograms derived from both distance matrix and parsimony analyses show the archaebacteria are a coherent group closely related to the eukaryotic nuclear RNA polymerase II and/or III lineages. The eukaryotic polymerase I lineage appears to arise separately from a bifurcation with the eubacterial beta' component lineage.
Genome-Wide Association Studies of Copy Number Variation in Autism Spectrum Disorder (2011)

Autism is a syndrome with a broad spectrum of phenotypes characterized by deficits in social interaction and communication, repetitive or stereotyped behaviors, and restricted interests Autism spectrum disorder (ASD) manifests mostly before 3 years of age ASDs include two related diagnoses; pervasive developmental disorder (PDD) including atypical autism, impairment in the same areas, but not meeting criteria for autism; Asperger's syndrome, which is milder than PDD, showing similar impairments in social interaction, behaviors and interests, but no significant delay in linguistic and cognitive development The prevalence rate of ASDs is ~0.6% and ASDs are approximately four times more common in males than in females (Veenstra-VanderWeele, 2004). Many studies have been performed to elucidate the pathogenesis of ASDs, but identified risk factors do not explain a significant proportion of the disease prevalence. Genetic epidemiological data have been suggesting that ASDs are heritable both in autism families and in the general population The concordance rates of autism in monozygotic twins were reported to be significantly higher (~ 60-90%) than those in dizygotic twins (~ 10%) and the recurrence rates are known to be approximately 10-20 times higher in siblings than in normal population ASD is not a single-gene disorder with Mendelian inheritance but rather a component of various genetic disorders with apparent cytogenetic abnormalities Cytogenetic alterations were detected in 7.4% of ASD However, discrepancies in study results and diverse modes of inheritance have hindered the discovery of common genetic susceptibility factors to ASDs. For these reasons, despite the growing evidence supporting the genetic susceptibility to ASD development Recent technical advance in microarray-based whole-genome analysis has enabled identification of common and rare genetic alterations associated with ASDs. Several recent studies have suggested that ASDs are associated with genetic variations including single nucleotide polymorphisms (SNPs) and copy number variations (CNVs), and that these genetic variations may work together (Veenstra-VanderWeele & Cook, 2004). For example, de novo CNVs were found in ~7% of idiopathic ASD families via oligoarray-comparative www.intechopen.com
Angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia (1998)

A number of heparin-binding growth factors, including basic (bFGF) and acidic (aFGF) fibroblast growth factors have been shown to promote angiogenesis in vivo. In this study, we employed a sustained-release polymer extravascular delivery system to evaluate the angiogenic efficacy of a novel form of genetically modified aFGF in the setting of chronic myocardial ischemia. Fifteen Yorkshire pigs subjected to Ameroid occluder placement on the left circumflex (LCX) artery were treated with perivascularly administered aFGF in ethylene vinyl acetate (EVAc) polymer (10 micrograms, n = 7) or EVAc alone (controls, n = 8). Seven to nine weeks later, after coronary angiography to document Ameroid-induced coronary occlusion, all animals underwent studies of coronary flow and global and regional left ventricular function. Microsphere-determined coronary flow in the Ameroid-compromised territory was significantly increased in aFGF-treated compared with control animals, and this improvement in perfusion was maintained during ventricular pacing. Left ventricular function studies demonstrated improved global and regional function in aFGF-treated animals. We conclude that local perivascular delivery of genetically modified aFGF results in significant improvement in myocardial flow and regional and global left ventricular function.
[Immunohistochemical study on the expression of epidermal growth factor receptor (EGF-R) in invasive cervical cancer of the uterus]. (1992)

We have investigated the expression of epidermal growth factor receptor (EGF-R) in invasive cervical cancer tissue of the uterus to determine whether there is a relationship between the expression of EGF-R and lymph node metastasis. Frozen sections of surgical specimens from twenty patients with invasive cervical cancer were immunohistochemically stained by the alkalinephosphatase anti-alkalinephosphatase method. The monoclonal antibody 528 we employed reacts with the receptor binding epitope within the EGF molecule, indicating that monoclonal antibody 528 is competitive with ligands such as EGF. Immunohistochemical staining was carried out before and after dissociation of ligands from the cellular surface of the tumor by acid treatment. However, the staining was resulted in no difference between before and after acid treatment. Consequently, the expression of EGF-R was detected in all cancer tissues as well in some normal tissue such as basal cell layers of epidermis. The expression of EGF-R was related inversely with lymph node metastasis by Wilcoxon rank sum test (p less than 0.05). These findings suggest that the expression of EGF-R in cervical cancer does not always lead to tumor growth or tumor invasion, although it does in some types of cancer.
A phase I study of two different schedules of nab-paclitaxel (nab-P) with ascending doses of vandetanib (V) with expansion in patients (Pts) with pancreatic cancer (PC). (2011)

4124 Background: nab-P is an albumin bound paclitaxel. V is a potent inhibitor of VEGFR 2, RET and EGFR. Preclinical data reveal enhanced nab-P activity with concurrent VEGF inhibition. Primary objective was to determine the maximum tolerated dose (MTD). A secondary objective was to assess preliminary efficacy in pts with PC. Methods: Pts were randomized to one of 2 cohorts. Dose of nab-P was fixed (Cohort A: nab-p 100 mg/m2 IV weekly for 3 wks q 28 days; Cohort B: nab-P 260 mg/m2 IV q 3 wks) while 3 doses of V were evaluated (100 mg, 200 mg, 300 mg orally daily). A 3+3 design was used. Expansion at the MTD (15 pts) was planned in both cohorts. Cohort A expansion was restricted to PC pts who failed gemcitabine based therapy. Two single nucleotide polymorphisms (SNPs), SPARC rs1059829 and rs3210714, were evaluated by PCR-RFLP on genomic DNA extracted from peripheral whole blood in 25 pts. Results: Dose escalation results were presented at ASCO 2010 (J Clin Oncol 28:15s, 2010 suppl; abstr 3059).There was one DLT at each dose level in cohort A (grade 3 rash in 2 pts, grade 4 neutropenia in 1 pt). No DLTs observed in cohort B. MTD was nab-P 100 mg/m2 IV weekly for 3 /4 wks and V 300 mg daily in cohort A; nab-P 260 mg/m2 IV q 3 wks and V 300 mg daily in cohort B. 29 pts with PC were treated: 9 (cohort A dose escalation), 15 (cohort A dose expansion) and 5 (cohort B). Median age was 62; 16 males, 13 females. Median number of prior treatments 2 (range 1-4). 22 pts with PC were evaluable for response: 6 (27.5%) PR, 10 (45%) SD and 6 (27.5%) PD. Median PFS was 5.3 mo (95% CI: 3.7-7.3) and median OS 8.2 mo (95% CI: 6.2 -11.5). No statistically significant association was found between SNPs in SPARC and clinical outcome. Conclusions: Both schedules of nab-P with V are well tolerated. The MTD for both cohorts is the maximum planned dose. The preliminary efficacy in a cohort of pts with refractory PC is promising and warrants further evaluation. Grade 3 or 4 toxicities for all pts in all cycles Cohort A% Cohort B% Neutropenia 31 48 Diarrhea 13 12 Leukopenia 10 16 Acneiform rash 15 8 Fatigue 10 4 Hypertension 5 12 Prolonged QTC 5 8 Thombosis 8 4 Joint pain 5 4 Muscle pain 5 4 Anorexia 5 0 Dyspnea 5 0 Nausea 5 0 Pruritus 5 0
Proopiomelanocortin Gene is Expressed in Many Normal Human Tissues and in Tumors not Associated with Ectopic Adrenocorticotropin Syndrome (1988)

Immunoreactive (IR) POMC peptides have been detected in several human nonpituitary tissues and most pheochromocytomas and lung cancers, including those not associated with ectopic ACTH syndrome. We found IR-ACTH, IR-gamma MSH, IR-beta-endorphin (beta END), and IR-lipotropin in extracts from the following 10 normal human tissues, listed in order of decreasing POMC peptide concentrations: adrenal, testis, spleen, kidney, ovary, lung, thyroid, liver, colon, and duodenum. IR-ACTH, IR-gamma MSH, and IR-beta END were detected in all six pheochromocytomas and all 12 lung tumors (six squamous cell carcinomas, five adenocarcinomas, and one small cell carcinoma) we examined, as well as in a squamous cell carcinoma of the larynx. None of the patients had clinical evidence of ectopic ACTH syndrome. To determine whether these nonpituitary tissues and tumors actually synthesize POMC, rather than simply absorb POMC peptides from plasma, we examined poly(A) RNA prepared from these tissues and total RNA from pituitary by Northern blot hybridization for the presence of POMC-like mRNA with an exon 3 riboprobe. Pituitary contained a single POMC mRNA species of about 1150 bases. A short POMC-like mRNA of about 900 bases was found in all normal nonpituitary tissues, three of five pheochromocytomas, eight of nine lung cancers, and the laryngeal squamous cell tumor. In addition, larger POMC-like mRNA species between 1200 to 1500 bases were detected in adrenal, testis, ovary, placenta, two pheochromocytomas, and three squamous cell lung tumors.(ABSTRACT TRUNCATED AT 250 WORDS)