gebhart/patrepeval huggingface dataset:
Distinct clinical and laboratory activity of two recombinant interleukin-2 preparations. (1999)

Interleukin-2 (IL-2) is a potent lymphokine that activates natural killer cells, T cells, and other cells of the immune system. Several distinct recombinant human IL-2 preparations have shown antitumor activity, particularly for renal cell cancer and melanoma. Somewhat distinct immune and clinical effects have been noted when different IL-2 preparations have been tested clinically; however, the regimens and doses used were not identical. To compare these more directly, we have evaluated two clinical recombinant IL-2 preparations in vitro and in vivo using similar regimens and similar IUs of IL-2. We used the Food and Drug Administration-approved, commercially available Chiron IL-2 and the Hoffmann LaRoche (HLR) IL-2 supplied by the National Cancer Institute. Using equivalent IUs of IL-2, we noted quantitative differences in vitro and in vivo in the IL-2 activity of these two preparations. In patients receiving comparable IUs of the two preparations, HLR IL-2 induced the release of more soluble IL-2 receptor alpha into the serum than Chiron IL-2. In addition, more toxicities were noted in patients receiving 1.5 x 10(6) IU of HLR IL-2 than were seen in patients treated with 1.5 x 10(6) or even 4.5 x 10(6) IU of Chiron IL-2. These toxicities included fever, nausea and vomiting, and hepatic toxicity. In vitro proliferative assays using IL-2-dependent human and murine cell lines indicated that the IU of HLR IL-2 was more effective than Chiron IL-2 at inducing tritiated thymidine incorporation. Using flow cytometry, we also found quantitative differences in the ability of these two preparations to bind to IL-2 receptors. These findings indicate that approximately 3-6 IU of Chiron IL-2 are required to induce the same biological effect as 1 IU of HLR IL-2.
Wideband Spectrum Sensing With Sub-Nyquist Sampling in Cognitive Radios (2012)

Multi-rate asynchronous sub-Nyquist sampling (MASS) is proposed for wideband spectrum sensing. Corresponding spectral recovery conditions are derived and the probability of successful recovery is given. Compared to previous approaches, MASS offers lower sampling rate, and is an attractive approach for cognitive radio networks.
Positioning of the RPR standard in contemporary operator environments (2004)

This article deals with the fundamentals and current standardization efforts for IEEE 802.17 resilient packet ring. Its special resilience features make this technology robust against outages of the network infrastructure. The goals of this article are threefold. First, the fundamentals of RPR and the standardization process carried out under the auspices of IEEE and ITU are overviewed. Second, potentially hazardous situations involving traffic assignments are defined and illustrated. Finally, possible situations where the simplicity, enhanced throughput, and automatic resilience features of RPR may be advantageous for network operators are identified.
Identification of coupled Markov chain model with application (2005)

A parameterized Markov chain model is developed to model the action of a biological ion channel measured in noise. In detail, the model takes the form of a set of binary chains which are interdependent according to a coupling parameter. When varied, this parameter realizes the range of behaviors from tight coupling to complete independence. Other parameters describe the intrinsic characteristics of the binary chains as well as their behavior when fully coupled. An identification procedure for the model parameters is developed based on hidden Markov modeling ideas but incorporating a novel parameter estimation step (due to the nonlinear form of the dependency of the model on the parameters). The model and identification methods are tested on real data.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Tone Reservation's complexity reduction using fast calculation of maximal IDFT element (2008)

International audience
Inhibition of glioma angiogenesis and growth in vivo by systemic treatment with a monoclonal antibody against vascular endothelial growth factor receptor-2. (2001)

Using an orthotopic intracerebral model, we investigated whether systemic treatment with DC101, a monoclonal antibody against vascular endothelial growth factor receptor (VEGFR)-2, could inhibit angiogenesis and the growth of human glioblastoma cells in severe combined immunodeficient mice. Intraperitoneal treatment with DC101, control IgG, or PBS was initiated either on day 0 or, in another series, on day 6 after tumor cell implantation, and animals were killed approximately 2 weeks after tumor cell injection. Tumor volumes in animals treated with DC101 were reduced by 59 and 81% compared with IgG and PBS controls, respectively (P < 0.001), when treatment was initiated immediately, and similar results were obtained when treatment started on day 6. Microvessel density in tumors of DC101-treated animals was reduced by at least 40% compared with animals treated with control IgG or PBS (P < 0.01). We observed a reduction in tumor cell proliferation and an increase in apoptosis in DC101-treated animals (P < 0.001). However, in mice treated with DC101, we also noticed a striking increase in the number and total area of small satellite tumors clustered around, but distinct from, the primary. These satellites usually contained central vessel cores, and tumor cells often had migrated over long distances along the host vasculature to eventually reach the surface and spread leptomeningeally. We conclude that systemic antagonization of VEGFR-2 can inhibit glioblastoma neovascularization and growth but can lead to increased cooption of preexistent cerebral blood vessels. Therefore, a combination of different treatment modalities which also include anti-invasive therapy may be needed for an effective therapy against glioblastoma, and the use of an antibody against VEGFR-2 may be one effective component.
Bronchial epithelial cell-cytokine interactions in airway inflammation. (1995)

A variety of cytokine bronchial cell interactions may play an important role in normal host defense as well as in the pathogenesis of inflammatory airway disorders such as asthma, cystic fibrosis, acute and chronic bronchitis, and bronchiectasis. First, airway epithelial cells may participate in local cytokine networks by synthesizing interleukins, chemokines, colony stimulating factors and growth factors in response to inflammatory mediators. Bronchial epithelial cell derived cytokines may thereby amplify ongoing inflammatory processes via the recruitment and activation of specific subsets of inflammatory cells, as well as by prolonging their survival in the airway microenvironment. Second, airway epithelial cells can initiate inflammatory cascades by generating cytokines in direct response to viral and bacterial products, noxious gases, and sensitizing chemicals. Third, airway epithelial cells represent targets for paracrine acting cytokines, which may then modulate bronchial epithelial cell functions. Finally, airway epithelial cells may modulate ongoing inflammatory events in the airway microenvironment via the shedding of soluble TNF receptors. Cytokine-bronchial epithelial cell interactions represent an important mechanism by which inflammatory events in the airway microenvironment can be regulated and represent potential targets for novel anti-inflammatory therapies in airway disorders.
A Novel UHF RFID Tag Antenna for Cigarette Carton (2011)

A novel UHF RFID tag antenna that can be seamlessly integrated into wrapping paper of metallic objects is reported. When designed for printing on cigarette carton with aluminum foil contained, both numerical simulation and measurement indicate that the proposed antenna has input impedance of (20 + j149) at resonant frequency of 915MHz, which provides satisfactory conjugate match to the Alien Higgs-2 RFID ASIC. The UHF RFID tag with designed antenna on cigarette carton demonstrates -10dB return loss bandwidth of 31MHz at the 915MHz UHF RFID band, and a read range of approximately 0.63m.
Diagnosis ofPneumocystis cariniiPneumonia: Improved Detection in Sputum with Use of Monoclonal Antibodies (1988)

With the dramatic increase in the frequency of Pneumocystis carinii pneumonia associated with human immunodeficiency virus infection, there has been a need for more rapid and less invasive diagnostic techniques. Recent studies have shown that examination of induced sputum can establish the diagnosis of P. carinii pneumonia in about 55 percent of cases. To assess whether a recently developed indirect immunofluorescent stain using monoclonal antibodies was more sensitive than Giemsa or toluidine blue O stains in detecting P. carinii in sputum, we undertook two prospective studies. Of 63 patients at one institution from whom sputum specimens were obtained, 49 were ultimately given a diagnosis of P. carinii pneumonia, 46 of them by staining of sputum. The sensitivity of the three stains in detecting P. carinii was 45 of 49 (92 percent) for immunofluorescence; 37 of 49 (76 percent) for Diff-Quik (a Giemsa-type stain); and 39 of 49 (80 percent) for toluidine blue O. There were no false positive immunofluorescent stains. In a similar study of a series of 25 patients at another institution, a diagnosis of P. carinii pneumonia was made in 23 of 25 patients by staining of induced sputum. We conclude that examination of induced sputum is a rapid, sensitive, and inexpensive method for diagnosing P. carinii pneumonia and that indirect immunofluorescence is a practical and highly sensitive staining technique for establishing this diagnosis.
Ischemic Brain Damage in Mice after Selectively Modifying BDNF or NT4 Gene Expression (2000)

The neurotrophins and the tyrosine kinase (Trk) B receptor may play a protective role in the pathophysiology of cerebral ischemia. In this study, the authors investigated whether reducing endogenous expression of TrkB-binding neurotrophins modifies the susceptibility to ischemic injury after 1-hour middle cerebral artery occlusion followed by 23 hours of reperfusion in a filament middle cerebral artery occlusion model. Mice lacking both alleles for neurotrophin-4 (nt4-/-) or deficient in a single allele for brain-derived neurotrophic factor (bdnf+/-) exhibited larger cerebral infarcts compared to wild-type inbred 129/SVjae mice (68% and 91%, respectively, compared to controls). Moreover, lesions were larger (21%) in nt4-/- mice after permanent middle cerebral artery occlusion. Hence, expression of both NT4 and BDNF, and by inference the TrkB receptor, confers resistance to ischemic injury.
Compression-tuned single-frequency Bragg grating fiber laser (1994)

Compressive stress has been used to continuously wavelength tune a single-frequency Bragg grating fiber laser over 32 nm without mode hopping. A master-oscillator/power-amplifier configuration and active noise reduction were implemented to maintain a constant, low-noise, 3-mW lasing power over the tuning range.
Sexual pharmacology in the 21st century. (2001)

Sexual dysfunction is highly prevalent in both sexes. Considerable progress has been made in the development of new pharmacologic treatments since the approval of sildenafil in 1998. A variety of oral erectogenic agents are available or are in late-phase development, including centrally active dopamine agonists (e.g., sublingual apomorphine), peripheral nonselective alpha-blockers (e.g., oral phentolamine), and other phosphodiesterase type-5 inhibitors (e.g., vardenafil). These drugs have recently been evaluated for the treatment of female sexual arousal disorder, although results to date have been inconclusive. Pharmacologic therapies have also been proposed for the treatment of premature ejaculation and hypoactive sexual desire disorder. Strong evidence exists for the value of serotonergic drugs (e.g., selective serotonin reuptake inhibitors) in the treatment of premature ejaculation. Further research is needed, particularly on the effects of these drugs on female sexual dysfunction.
A polypeptide factor produced by fibrosarcoma cells that induces endothelial tissue factor and enhances the procoagulant response to tumor necrosis factor/cachectin. (1990)

Intravascular clot formation, localized to the neoplasm, is an early component of the vascular response to tumor necrosis factor (TNF)/cachectin. Fibrin is closely associated with the endothelial cell surface, and multiple microthromboses lead to reduced blood flow in the tumor. We have identified a tumor-derived mediator which enhances endothelial procoagulant activity and the cellular response to TNF using cultured cells derived from a murine methylcholanthrene A (meth A)-induced fibrosarcoma as a model system. A heat-stable protease K-sensitive polypeptide, Mr approximately 44,000 on nonreduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Mr approximately 56,000 reduced), was purified approximately 500,000-fold from serum-free culture supernatants of meth A cells by sequential Q-Sepharose, Mono S, reversed phase, and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Based on immunologic criteria, biologic activity, and other molecular properties, meth A factor appears to be distinct from other cytokines and growth factors. Purified meth A factor induced transcription of the tissue factor gene and expression of procoagulant activity by cultured human endothelium (half-maximal effect for the latter at approximately 6-8 pM). Furthermore, co-incubation of endothelium with meth A factor together with TNF enhanced induction of tissue factor in a more than additive manner. These data indicate that certain tumors elaborate an apparently unique molecule which can alter hemostatic properties of the vessel wall, potentially modulating reactivity of the tumor vasculature to host response mediators.
Immunohistochemical analysis of bcl-2, bax, bcl-X, and mcl-1 expression in prostate cancers. (1996)

Proteins encoded by bcl-2 family genes are important regulators of programmed cell death and apoptosis. Alterations in the expression of these apoptosis-regulating genes can contribute to the origins of cancer, as well as adversely influence tumor responses to chemo- and radiotherapy. Using antibodies specific for the Bcl-2, Bax, Bcl-X, and Mcl-1 proteins in combination with immunohistochemical methods, we examined for the first time the expression of these bcl-2 family genes in 64 cases of adenocarcinoma of the prostate, including 10 Gleason grade 2 to 4 tumors, 21 grade 5 to 7 tumors, 17 grade 8 to 10 tumors, 8 lymph node metastases, and 8 bone metastases. In addition, 24 cases of prostatic intraepithelial neoplasia (PIN) or PIN coexisting with carcinoma were also evaluated. All immunostaining results were scored with regard to approximate percentage of positive tumor cells and relative immunostaining intensity. Expression of the anti-apoptotic protein Bcl-2 was present in 16 of 64 (25%) adenocarcinomas and tended to be more frequent in high grade tumors (Gleason grade 8 to 10; 41%) and nodal metastases (38%) than in lower grade (Gleason 2 to 7) primary tumors (16%; P < 0.05). Bcl-X was expressed in all 64 (100%) tumors evaluated. Bcl-X immunointensity was generally stronger in high grade primary tumors (grade 8 to 10) and metastases compared with PIN and low grade neoplasms (P < 0.0001). In addition, the proportion of specimens with > 50% Bcl-X-immunopositive tumor cells also was higher in advanced grade primary tumors (Gleason 8 to 10) and metastases than in PIN and low grade tumors (Gleason 2 to 7; P < 0.005). The anti-apoptotic protein Mcl-1 was expressed in 52 of 64 (81%) tumors, compared with only 9 of 24 (38%) cases of PIN (P < 0.001). In addition, the percentage of Mcl-1-positive cells was typically higher in Gleason grade 8 to 10 tumors and metastases than in PIN or lower grade tumors (P = 0.025). In contrast, the pro-apoptotic protein Bax was expressed in all prostate cancers evaluated, with high percentages of immunopositive cells and strong immunointensity typically occurring regardless of tumor grade. The findings suggest that expression of several anti-apoptotic members of the bcl-2 gene family, including bcl-2, bcl-X, and mcl-1 increases during progression of prostate cancers, a finding that may be relevant to the hormone-insensitive, metastatic phenotype of most advanced adenocarcinomas of the prostate.
Admission control for VoIP traffic in IEEE 802.11 networks (2004)

In this paper, we propose a metric for measuring the utilization of an IEEE 802.11b wireless network and outline how this metric can be accurately estimated using data that is readily available in most access points. We furthermore describe how this metric can be used to perform admission control for VoIP traffic and describe experiences with a prototype implementation. Admission control for VoIP traffic in 802.11 networks is necessary since the number of simultaneous VoIP connections in a single cell of an 802.11 network is very small.
Tunable Touch Sensor and Combined Sensing Platform: Toward Nanoparticle-based Electronic Skin (2013)

In this paper, we present touch (or pressure) flexible sensors based on monolayer-capped nanoparticles (MCNPs) that are potentially inexpensive, could allow low-voltage operation, and could provide a platform for multifunctional applications. We show that modifying the mechanical and geometrical properties of the flexible substrates, on which the MCNP films are deposited, allows measuring a large span of loads ranging between tens of mg to tens of grams. All flexible sensors exhibited repeatable responses even after a large number of bending cycles. In addition, we show that modified platforms of those touch (or pressure) sensors allow precise detection and monitoring of environmental temperature and humidity. Relying on their superior characteristics, we were able to build an MCNP-based prototype allowing simultaneous detection and monitoring of multiple environmental parameters of touch (or pressure), humidity, and temperature. The excellent temperature (resolution higher than 1 °C and average error of ~5%) and relative humidity (resolution higher than 1% RH and average error of ~9%) sensitivities and the possibility to integrate those sensing abilities makes the suggested platform interesting for potentially inexpensive and low-voltage multifunctional electronic-skin applications.
Low level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts (2009)

Despite over forty years of investigation on low-level light therapy (LLLT), the fundamental mechanisms underlying photobiomodulation remain unclear. In this study, we isolated murine embryonic fibroblasts (MEF) from transgenic NF-kB luciferase reporter mice and studied their response to 810-nm laser radiation. Significant activation of NFkB was observed for fluences higher than 0.003 J/cm<sup>2</sup>. NF-kB activation by laser was detectable at 1-hour time point. Moreover, we demonstrated that laser phosphorylated both IKK &alpha;/&beta; and NF-kB 15 minutes after irradiation, which implied that laser activates NF-kB via phosphorylation of IKK &alpha;/&beta;. Suspecting mitochondria as the source of NF-kB activation signaling pathway, we demonstrated that laser increased both intracellular reactive oxygen species (ROS) by fluorescence microscopy with dichlorodihydrofluorescein and ATP synthesis by luciferase assay. Mitochondrial inhibitors, such as antimycin A, rotenone and paraquat increased ROS and NF-kB activation but had no effect on ATP. The ROS quenchers N-acetyl-L-cysteine and ascorbic acid abrogated laser-induced NF-kB and ROS but not ATP. These results suggested that ROS might play an important role in the signaling pathway of laser induced NF-kB activation. However, the western blot showed that antimycin A, a mitochondrial inhibitor, did not activate NF-kB via serine phosphorylation of IKK &alpha;/&beta; as the laser did. On the other hand, LLLT, unlike mitochondrial inhibitors, induced increased cellular ATP levels, which indicates that light also upregulates mitochondrial respiration. ATP upregulation reached a maximum at 0.3 J/cm<sup>2</sup> or higher. We conclude that LLLT not only enhances mitochondrial respiration, but also activates the redox-sensitive transcription factor NF-kB by generating ROS as signaling molecules.
Recent advances in genome editing and creation of genetically modified pigs (2015)

The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.
Accurate recovery of three-dimensional shape from image focus (1995)

A new shape-from-focus method is described which is based on a new concept, named focused image surface (FIS). FIS of an object is defined as the surface formed by the set of points at which the object points are focused by a camera lens. According to paraxial-geometric optics, there is a one-to-one correspondence between the shape of an object and the shape of its FIS. Therefore, the problem of shape recovery can be posed as the problem of determining the shape of the FIS. From the shape of FIS the shape of the object is easily obtained. In this paper the shape of the FIS is determined by searching for a shape which maximizes a focus measure. In contrast to previous literature where the focus measure is computed over the planar image detector of the camera, here the focus measure is computed over the FIS. This results in more accurate shape recovery than the traditional methods. Also, using FIS, a more accurate focused image can be reconstructed from a sequence of images than is possible with traditional methods. The new method has been implemented on an actual camera system, and the results of shape recovery and focused image reconstruction are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Efficient introduction of alkene functionality into proteins in vivo (1998)

The methionine analogue 2-amino-5-hexenoic acid (homoallylglycine, Hag) can be utilized by Escherichia coli in the initiation and elongation steps of protein biosynthesis. Use of an E. coli methionine auxotroph and Hag-supplemented medium resulted in replacement of ca. 85% of the methionine residues in mouse dihydrofolate reductase expressed under control of a bacteriophage T5 promoter. N-terminal sequencing indicated 92+/-5% occupancy of the initiator site by Hag. The vinyl function of Hag remains intact in the purified protein and suggests new chemistries for modification of natural and artificial proteins prepared in bacterial hosts.
Immunologic "vaccination" for the prevention of autoimmune diabetes (type 1A). (1999)

Diabetes type 1A is an autoimmune condition characterized by lymphocytic infiltration of islets and selective destruction of insulin-secreting beta-cells. Numerous investigators have prevented diabetes in animal models with a variety of antigens and routes of administration. It is also now possible to identify high-risk individuals even before the appearance of autoantibodies. These advances have created the opportunity to design and begin human prevention trials. This review focuses on a variety of immunomodulatory approaches (including administration of adjuvants, autoantigens, T-cells, T-cell receptors, and DNA) that we have collectively termed immunologic "vaccination." In addition, we discuss the potential benefits and dangers of these approaches and issues relating to the design of human trials.