gebhart/patrepeval huggingface dataset:
Oxidative stress, antioxidant status and DNA damage in patients with impaired glucose regulation and newly diagnosed Type 2 diabetes (2007)

Previous studies have postulated the association between oxidative stress and Type 2 diabetes. Considering the long pre-diabetic period with IGR (impaired glucose regulation) and its high risk of developing diabetes, to test this hypothesis, we have investigated oxidative stress pathways and DNA damage in patients with IGR and newly diagnosed Type 2 diabetes. The study population consisted of 92 subjects with NGT (normal glucose tolerance), 78 patients with IGR and 113 patients with newly diagnosed diabetes. Plasma MDA (malondialdehyde) and TAC (total antioxidative capacity) status, erythrocyte GSH content and SOD (superoxide dismutase) activity were determined. A comet assay was employed to evaluate DNA damage. Compared with subjects with NGT, patients with IGR had reduced erythrocyte SOD activity. Patients with diabetes had a higher plasma MDA concentration, but a lower plasma TAC level and erythrocyte SOD activity, than the NGT group. Correlation analysis revealed a strong positive association between IR (insulin resistance) and MDA concentration, but negative correlations with TAC status and SOD activity. With respect to beta-cell function, a positive association with TAC status and an inverse correlation with GSH respectively, were observed. The comet assay revealed slight DNA damage in patients with IGR, which was increased in patients with diabetes. Significant correlations were observed between DNA damage and hyperglycaemia, IR and beta-cell dysfunction. In conclusion, the results of the present study suggest that hyperglycaemia in an IGR state caused the predominance of oxidative stress over antioxidative defence systems, leading to oxidative DNA damage, which possibly contributed to pancreatic beta-cell dysfunction, IR and more pronounced hyperglycaemia. This vicious circle finally induced the deterioration to diabetes.
Status of the Midwest Proton Radiotherapy Institute (2004)

Phase I construction of the Indiana University Midwest Proton Radiotherapy Institute (MPRI), including many reliability upgrades to the IUCF k220 cyclotron, construction of a variable energy proton Beam Delivery System (BDS), a horizontal beam Treatment Room with a Large Field Nozzle, and a Medical Clinic, is now complete. Commissioning of the BDS and the Large Field Nozzle is underway. Patient Treatment with this nozzle is scheduled to begin in late July 2003. This contribution discusses the unique features of the MPRI Cancer Treatment facility and the present Phase I commissioning results. In addition, two 360/spl deg/ rotating gantry systems were purchased from IBA in December 2002 for Phase II construction. The first gantry is scheduled for delivery in July 2003. The Phase II construction and installation schedule will also be briefly presented.
Steroids block the anti-inflammatory effects of low level laser therapy (2006)

Objective: Concomitant use of multiple therapies is common in musculoskeletal and airway disorders. Low level laser therapy (LLLT) is considered a promising therapy in arthritis, tendinopathies and rhinitis. We designed two animal studies to assess if the expected anti-inflammatory effect LLLT could be affected by resection of the adrenal gland or concomitant use of the cortisol antagonist mifepristone. Methods: Two studies were performed, with 40 male Wistar rats and with 40 Balb C male mice respectively.. In both studies, four groups received carrageenan and one control group received saline. At 1, 2, and 3 hours after injections, LLLT irradiation was performed with a dose of 7.5 J/cm<sup>2</sup>. In the rat study, two of the carrageenan groups had the adrenal gland dissected. In the mice study, two of the carrageenan-injected groups were in addition pre-treated with orally administered mifepristone. Results: In the rat paw study, LLLT reduced edema significantly compared to the carrageenan only group (1.5 vs 0.9 ml, p< 0.05), but LLLT failed to inhibit edema formation in the group which had the adrenal gland resected. In carrageenan-induced pleurisy, LLLT significantly reduced the number of leukocyte cells ( p<0.0001, Mean 34.5 [95%CI: 32.8 - 36.2] versus 87.7 [95%CI: 81.0 - 94.4]), and that the effect of LLLT could be totally blocked by adding the cortisol antagonist mifepristone ( p<0.0001, Mean 34.5 [95%CI: 32.1 - 36.9] versus 82.9 [95%CI: 70.5 - 95.3]). Conclusion: Steroid therapy should not be used concomitantly with LLLT, as the anti-inflammatory effect of LLLT is lost if cortisol receptors are downregulated.
Mitogen-activated Protein Kinases in Inflammation (2012)

Mitogen-activated protein kinases (MAPKs) play critical regulatory roles in the production of the pro-inflammatory cytokines and downstream signaling events which lead to inflammation. Inflammation is a primarily localized and protective response of host against microbial infection. Controlled inflammation is beneficial and necessary for host defense while uncontrolled inflammatory response results in inflammatory diseases such as septic shock, rheumatoid arthritis and cancer. The MAPK family consists of three subfamilies; the extracellular signal-regulated kinases (ERKs), the c-Jun N-terminal kinases (JNKs), and the p38 MAPKs. MAPKs are involved in transmitting extracellular signals to nucleus which leads to gene regulation. In this review, we summarize the current knowledge of ERK1/2, JNKs, and p38 MAPK members and their roles in inflammation.
Therapeutic Potential of Peroxisome Proliferator-Activated Receptor Agonists for Neurological Disease (2003)

Activation of peroxisome proliferator-activated receptors (PPARs) mediates the insulin-sensitizing effects of thiazolidinediones used for treatment of type 2 diabetes, owing to changes in the transcription and expression of genes influencing carbohydrate and lipid metabolism. However, PPAR activation can have additional effects upon cellular physiology, including anti-proliferative and anti-inflammatory. These effects are observed in many cell types, including brain glial cells and blood lymphocytes, cells whose activation contributes to the initiation and progression of damage occurring in neurological diseases such as Alzheimer's disease (AD) and multiple sclerosis (MS). In view of the need for development of additional therapeutic options, several recent studies have tested the possibility that PPAR agonists would be neuroprotective in these diseases. This paper will summarize data from cell culture experiments and from studies in animal models, demonstrating that PPARgamma agonists can exert neuroprotective effects, thereby providing the basis for the design of clinical trials to test the safety and efficacy of thiazolidinediones in neuroinflammatory conditions such as AD and MS.
The Work and Social Adjustment Scale: a simple measure of impairment in functioning (2002)

The WSAS is a simple, reliable and valid measure of impaired functioning. It is a sensitive and useful outcome measure offering the potential for readily interpretable comparisons across studies and disorders.
Evidence for in vivo transport of bioactive nitric oxide in human plasma (2002)

Although hitherto considered as a strictly locally acting vasodilator, results from recent clinical studies with inhaled nitric oxide (NO) indicate that NO can exert effects beyond the pulmonary circulation.We therefore sought to investigate potential remote vascular effects of intra-arterially applied aqueous NO solution and to identify the mechanisms involved.On bolus application of NO into the brachial artery of 32 healthy volunteers, both diameter of the downstream radial artery and forearm blood flow increased in a dose-dependent manner.Maximum dilator responses were comparable to those after stimulation of endogenous NO formation with acetylcholine and bradykinin.Response kinetics and pattern of NO decomposition suggested that despite the presence of hemoglobin-containing erythrocytes, a significant portion of NO was transported in its unbound form.Infusion of NO (36 mol/min) into the brachial artery increased levels of plasma nitroso species, nitrite, and nitrate in the draining antecubital vein (by < 2-fold, 30-fold, and 4-fold, respectively), indicative of oxidative and nitrosative chemistry.Infused N-oxides were inactive as vasodilators whereas S-nitrosoglutathione dilated conduit and resistance arteries.Our results suggest that NO can be transported in bioactive form for significant distances along the vascular bed.Both free NO and plasma nitroso species contribute to the dilation of the downstream vasculature.
A robust timing and frequency synchronization for OFDM systems (2003)

A robust symbol-timing and carrier-frequency synchronization scheme applicable to orthogonal frequency-division-multiplexing systems is presented. The proposed method is based on a training symbol specifically designed to have a steep rolloff timing metric. The proposed timing metric also provides a robust sync detection capability. Both time domain training and frequency domain (FD) training are investigated. For FD training, maintaining a low peak-to-average power ratio of the training symbol was taken into consideration. The channel estimation scheme based on the designed training symbol was also incorporated in the system in order to give both fine-timing and frequency-offset estimates. For fine frequency estimation, two approaches are presented. The first one is based on the suppression of the interference introduced in the frequency estimation process by the training symbol pattern in the context of multipath dispersive channels. The second one is based on the maximum likelihood principle and does not suffer from any interference. A new performance measure is introduced for timing estimation, which is based on the plot of signal to timing-error-induced average interference power ratio against the timing estimate shift. A simple approach for finding the optimal setting of the timing estimator is presented. Finally, the sync detection, timing estimation, frequency estimation, and bit-error-rate performance of the proposed method are presented in a multipath Rayleigh fading channel.
Identification of an iron-regulated outer membrane protein of Neisseria meningitidis involved in the utilization of hemoglobin complexed to haptoglobin (1995)

Hemoglobin complexed to the plasma protein haptoglobin can be used by Neisseria meningitidis as a source of iron to support growth in vitro. An N meningitidis mutant, DNM2E4, was generated by insertion of the mini-Tn3erm transposon into the gene coding for an 85-kDa iron-regulated outer membrane protein. Membrane proteins prepared from DNM2E4 were identical to those of the wild-type strain except that the 85-kDa protein was not produced. This mutant was unable to use hemoglobin-haptoglobin complexes as an iron source to support growth and was also impaired in the utilization of free hemoglobin. The mutant failed to bind free hemoglobin, hemoglobin-haptoglobin complexes, or apo-haptoglobin in a solid-phase dot blot assay. The 85-kDa protein was affinity purified when hemoglobin-haptoglobin complexes were used as a ligand but was not purified when free hemoglobin was used. We hypothesize that the 85-kDa iron-regulated protein is the hemoglobin-haptoglobin receptor and designate this protein Hpu (for hemoglobin-haptoglobin utilization).
Copper ions as inhibitors of protein C of soluble methane monooxygenase of Methylococcus capsulatus (Bath) (1985)

Copper(I), copper(II) and silver ions have been shown to be potent inhibitors of purified soluble methane monooxygenase (MMO) of Methylococcus capsulatus (Bath). A weaker inhibition has been observed with zinc and cadmium ions. Proteins A and B of soluble MMO are unaffected by copper but protein C is rapidly and irreversibly inhibited. The site of copper inhibition has been shown to be primarily at the iron-sulphur centre of protein C with a secondary effect at the FAD centre when the copper(II):protein C ratio is high. Copper appears to bring about the inhibition of soluble MMO by interacting with protein C to disrupt the protein structure causing, firstly, the loss of the iron-sulphur centre, preventing the transfer of electrons from protein C to protein A, and secondly, the loss of FAD preventing the protein from accepting electrons from NADH. Inhibition and spectral data are provided to support this thesis. The inactivation of protein C is associated with the tight binding of four Cu atoms to each protein C molecule. These data extend our knowledge of how copper, which is known to have a key role in the cellular location of MMO, interacts with and rapidly and irreversibly inactivates the soluble form of this enzyme.
Electrohydraulic Discharge and Nonthermal Plasma for Water Treatment (2005)

The application of strong electric fields in water and organic liquids has been studied for several years, because of its importance in electrical transmission processes and its practical applications in biology, chemistry, and electrochemistry. More recently, liquid-phase electrical discharge reactors have been investigated, and are being developed, for many environmental applications, including drinking water and wastewater treatment, as well as, potentially, for environmentally benign chemical processes. This paper reviews the current status of research on the application of high-voltage electrical discharges for promoting chemical reactions in the aqueous phase, with particular emphasis on applications to water cleaning.
Combination Therapy with Losartan and Pioglitazone Additively Reduces Renal Oxidative and Nitrative Stress Induced by Chronic High Fat, Sucrose, and Sodium Intake (2012)

We recently showed that combination therapy with losartan and pioglitazone provided synergistic effects compared with monotherapy in improving lesions of renal structure and function in Sprague-Dawley rats fed with a high-fat, high-sodium diet and 20% sucrose solution. This study was designed to explore the underlying mechanisms of additive renoprotection provided by combination therapy. Losartan, pioglitazone, and their combination were orally administered for 8 weeks. The increased level of renal malondialdehyde and expression of nicotinamide adenine dinucleotide phosphate oxidase subunit p47(phox) and nitrotyrosine as well as the decreased total superoxide dismutase activity and copper, zinc-superoxide dismutase expression were tangible evidence for the presence of oxidative and nitrative stress in the kidney of model rats. Treatment with both drugs, individually and in combination, improved these abnormal changes. Combination therapy showed synergistic effects in reducing malondialdehyde level, p47(phox), and nitrotyrosine expression to almost the normal level compared with monotherapy. All these results suggest that the additive renoprotection provided by combination therapy might be attributed to a further reduction of oxidative and nitrative stress.
Identification of a 35-kilodalton Mycobacterium tuberculosis protein containing B- and T-cell epitopes (1990)

Screening of a Mycobacterium tuberculosis genomic DNA library in the lambda gt11 expression vector was carried out by using, as probes, sera from tuberculous patients and murine monoclonal antibody H61.3 recognizing a mycobacterial 35-kilodalton protein present only on the M. tuberculosis complex. The recombinant beta-galactosidase-fused protein present in the crude lysate induced the proliferation of T lymphocytes from patients with tuberculous pleuritis. As the recombinant insert contains an internal EcoRI restriction site, it was possible to identify two fragments, one proximal to the lacZ gene and 1.7 kilobases (kb) in size and the other distal to the lacZ gene and 2.2 kb in size. Southern blot analysis showed that both of them hybridized with the genomic DNA from M. tuberculosis and M. bovis but not with the DNA from other mycobacterial species. To perform extensive immunological studies, the amount of beta-galactosidase-fused protein being very low, we fused the 1.7-kb fragment to the N-terminal part of the gene coding for the DNA polymerase of bacteriophage MS2 in the expression vector pEx34. The fusion protein was partially purified, and subsequent Western blotting (immunoblotting) and T-cell proliferation experiments confirmed the presence of B- and T-cell mycobacterial epitopes. Furthermore, to isolate the chromosomal region containing the 35-kilodalton gene, we constructed another mycobacterial genomic library in the lambda 2001 vector by cloning 15 to 20 kb of foreign DNA. Screening of this library was carried out by using 1.7- and 2.2-kb recombinant fragments as probes. Restriction maps of some clones isolated were determined.
An Electronic Medical Record-Based Model to Predict 30-Day Risk of Readmission and Death Among HIV-Infected Inpatients (2012)

Readmission rates among HIV-infected patients were high. An automated model composed of factors accessible from the EMR in the first 48 hours of admission performed well in predicting the 30-day risk of readmission among HIV patients. Such a model could be used in real-time to identify HIV patients at highest risk so readmission prevention resources could be targeted most efficiently.
SLM and PTS peak-power reduction of OFDM signals without side information (2005)

Selected mapping (SLM) and partial transmit sequence (PTS) are well-known techniques for peak-power reduction in orthogonal frequency-division multiplexing (OFDM). We derive a simplified maximum likelihood (ML) decoder for SLM and PTS that operates without side information. This decoder exploits the fact that the modulation symbols belong to a given constellation and that the multiple signals generated by the PTS or SLM processes are widely different in a Hamming distance sense. Pairwise error probability (PEP) analysis suggests how SLM and PTS vectors should be chosen. The decoder performs well over additive white Gaussian noise (AWGN) channels, fading channels, and amplifier nonlinearities.
Implementation of HfSiON gate dielectric for sub-60nm DRAM dual gate oxide with recess channel array transistor(RCAT) and tungsten gate (2005)

In this work, HfSiON gate dielectric is integrated for the first time in dual gate oxide of DRAM with recess channel arrary transistor (RCAT) and W/poly-Si gate for the development of sub-60nm DRAM technology. No degradation of cell transistor characteristics was observed with HfSiON gate dielectric. In peripheral transistors, excellent sub-threshold swings and driving current of 515 /spl mu/A//spl mu/m and 216 /spl mu/A//spl mu/m for nMOS and pMOS, respectively, at V/sub dd/=1.8V and I/sub off/=20/spl mu/A//spl mu/m were obtained. Compared to surface channel pMOSFET, lower V/sub th/ was achieved in buried channel pMOSFET due to fermi-level pinning. Negligible increase of gate leakage current during post annealing up to 950/spl deg/C for 30min is shown the excellent thermal stability of HfSiON dielectric.
PD-L1 Expression Is Characteristic of a Subset of Aggressive B-cell Lymphomas and Virus-Associated Malignancies (2013)

Certain aggressive B-cell lymphomas and virus- and immunodeficiency-associated malignancies associated with an ineffective T-cell immune response express PD-L1 on tumor cells and infiltrating macrophages. These results identify a group of neoplasms that should be considered for PD-1/PD-L1-directed therapies, and validate methods to detect PD-L1 in FFPE tissue biopsies.
Vaccine and Monoclonal Antibody That Enhance Mouse Resistance to Candidiasis (2011)

Previously we showed that antibodies specific for the glycan β-1,2-mannotriose [β-(Man)(3)] on the cell surface of Candida albicans protect mice against disseminated candidiasis (H. Xin, S. Dziadek, D. R. Bundle, and J. E. Cutler, Proc. Natl. Acad. Sci. U. S. A. 105:13526-13531, 2008). Furthermore, six 14-mer peptides that are within the N-terminal portion of C. albicans wall proteins were conjugated to the glycan in an attempt to create immunogenic glycopeptide conjugates. By a dendritic cell (DC)-based immunization approach, all were immunogenic and three of the six conjugates induced a high degree of protection in mice. Interestingly, whereas all six peptides induced antibody responses when used alone to pulse DCs for subsequent immunizations, three peptides induced protection, and one in particular, peptide Fba (derived from fructose-bisphosphate aldolase), induced robust protective responses and is the focus of the current work. Fba peptide is not restricted by the major histocompatibility complex class II (MHC-II), as it induced anti-Fba antibodies in mice of different H-2 haplotypes and in rabbits. Furthermore, the peptide induced protection against disease caused by different C. albicans strains. Partial protection was achieved when alum was used in place of DCs for Fba immunizations. The passive transfer of immune sera from Fba-vaccinated mice, but not immune serum preabsorbed with fungal cells, conferred protection in naïve mice. This result, along with our finding that a monoclonal antibody specific for the peptide, E2-9 (IgM), protected mice against candidiasis, provide strong evidence that antibodies contribute to protection. Our work demonstrates the utility of cell wall peptides alone or as glycopeptides in vaccines designed for the induction of immunity against candidiasis and monoclonal antibodies as a rapid immunoprotective approach against the disease.
Nonlinear anisotropic filtering of MRI data (1992)

In contrast to acquisition-based noise reduction methods a postprocess based on anisotropic diffusion is proposed. Extensions of this technique support 3-D and multiecho magnetic resonance imaging (MRI), incorporating higher spatial and spectral dimensions. The procedure overcomes the major drawbacks of conventional filter methods, namely the blurring of object boundaries and the suppression of fine structural details. The simplicity of the filter algorithm permits an efficient implementation, even on small workstations. The efficient noise reduction and sharpening of object boundaries are demonstrated by applying this image processing technique to 2-D and 3-D spin echo and gradient echo MR data. The potential advantages for MRI, diagnosis, and computerized analysis are discussed in detail.