gebhart/patrepeval huggingface dataset:
RECURRENCE PATTERNS AFTER RADICAL RETROPUBIC PROSTATECTOMY: CLINICAL USEFULNESS OF PROSTATE SPECIFIC ANTIGEN DOUBLING TIMES AND LOG SLOPE PROSTATE SPECIFIC ANTIGEN (1997)

After PSA became detectable PSA doubling time or, equivalently, log slope PSA, was a better indicator of the risk and time to clinical recurrence after radical retropubic prostatectomy than preoperative PSA, specimen Gleason sum or pathological stage. Hormone treatment may be targeted to patients at high risk for early metastatic clinical recurrence, appropriately timed radiation can be offered for proved local recurrence in those with long PSA doubling time and expectant treatment may be proposed for those with long PSA doubling time who remain clinically disease-free. Frequent and expensive imaging does not appear to be cost-effective in this latter group.
[recension av:] Allan H. Charnley: Paid Educational Leave. A report of practice in France, Germany and Sweden, London 1975 (1978)

[recension av:] Allan H. Charnley: Paid Educational Leave. A report of practice in France, Germany and Sweden, London 1975
Human secretin (SCT): gene structure, chromosome location, and distribution of mRNA (2000)

Secretin is an endocrine hormone that stimulates the secretion of bicarbonate-rich pancreatic fluids. Recently, it has been discussed that secretin deficiency may be implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. In the present study, the human secretin gene (SCT) was isolated from a bacterial artificial chromosome genomic library. SCT contains four exons, with the protein coding regions spanning 713 bp of genomic DNA. Human SCT is similar structurally to the secretin genes of other species. Amino acid conservation, however, is most pronounced within the exon encoding the biologically active mature peptide. Northern blot analysis shows that human SCT transcripts are located in the spleen, intestinal tract, and brain. Radiation hybrid mapping places the SCT locus on chromosome 11p15.5.
Aligned Carbon Nanotube Film Enables Thermally Induced State Transformations in Layered Polymeric Materials (2015)

The energy losses and geometric constraints associated with conventional curing techniques of polymeric systems motivate the study of a highly scalable out-of-oven curing method using a nanostructured resistive heater comprised of aligned carbon nanotubes (A-CNT). The experimental results indicate that, when compared to conventional oven based techniques, the use of an "out-of-oven" A-CNT integrated heater leads to orders of magnitude reductions in the energy required to process polymeric layered structures such as composites. Integration of this technology into structural systems enables the in situ curing of large-scale polymeric systems at high efficiencies, while adding sensing and control capabilities.
Molecular pathogenesis of Parkinson's disease (2005)

Parkinson's disease (PD) is a common and incurable neurodegenerative disease, affecting 1% of the population over the age of 65. Despite a well-described clinical and pathological phenotype, the molecular mechanisms which lead to neurodegeneration remain elusive. However, there is a wealth of evidence from both toxin based models and genetic based models, which suggest a major etiologic role for mitochondrial dysfunction, protein aggregation, the ubiquitin-proteasome system and kinase signalling pathways in the pathogenesis of PD. Ultimately, an understanding of the molecular events which precipitate neurodegeneration in idiopathic PD will enable the development of targeted and effective therapeutic strategies. We review the latest evidence for the proposed molecular processes and discuss their relevance to the pathogenesis of sporadic PD.
Phase Transitions of Mixtures of Amorphous Polysaccharides and Sugars (1991)

Abstract Maltodextrins of varying molecular weights, maltose, and sucrose were used to study the effect of molecular weight, water plasticization, and composition on glass transition temperature ( T g ). All maltodextrins were plasticized by water, and the decrease of T g was linear with water activity over the range of 0.11–0.85. The plasticization effect of water was similar for maltodextrins having various molecular weights. Effects of molecular weight and composition on the T g of maltodextrins could be correlated by using relationships reported previously for polymers. The quantitative results obtained can be applied to formulate food and related materials having desired processability and storage stability.
Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells. (1982)

Mitochondria in cardiac muscle cells and myoblast-fused myotubes display unusually long (3-5 days) retention times of rhodamine 123, a mitochondria-specific fluorescent probe, in living cells. Among 50 keratin-positive carcinoma or transformed epithelial cell lines tested, mitochondria with prolonged rhodamine 123 retention are detected in most of the transitional cell carcinoma, adenocarcinoma, and chemical carcinogen-transformed epithelial cell lines and in some squamous cell carcinoma lines but not in any oat cell carcinoma lines. The presence of mitochondria having unusual dye retention may be useful for diagnosis and exploitable for chemotherapy of certain human carcinomas.
Expression of vitreous cytokines in proliferative vitreoretinopathy: a prospective study. (1999)

The various cytokines may play a role in the pathobiology of PVR. High vitreous levels of IL-6 and protein were identified as significant risk factors for PVR. A model was developed to predict the probability of development of postoperative PVR in these patients, and it may be used to indicate intravitreal pharmacologic treatment for those at risk.
DSS Access on the WWW: An Intelligent Agent Prototype (1998)

This paper describes a prototype DSS Discovery Agent, an intelligent agent designed to facilitate access to DSS deployed using an Open DSS protocol. The agent utilizes a set of specialized HTML metainformation headers to determine whether a given Web site contains a Decision Support System (DSS) and whether that DSS meets user defined requirements.
High‐Yielding Microwave‐Assisted Synthesis of Triazole‐Linked Glycodendrimers by Copper‐Catalyzed [3+2] Cycloaddition (2005)

Abstract A facile and high‐yielding synthesis of multivalent 1,4‐disubstituted 1,2,3‐triazole‐linked glycodendrimers is described. Azido carbohydrates are linked by a Cu I ‐catalyzed [3+2] cycloaddition reaction to dendritic acetylenes using microwave irradiation. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2005)
A Practical Sulfenylation of 2,5‐Diketopiperazines (2011)

Sulfurs in action: A practical and simple method has been developed for the introduction of sulfur atoms into 2,5-diketopiperazines (I) under mild conditions. The reaction provides more or less complex epidithiodiketopiperazines (II) and bis-methylthiodiketopiperazines (III). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Co-assembly of plasma and cellular fibronectins into fibrils in human fibroblast cultures. (1990)

Exogenous plasma and endogenous cellular fibronectins on the surface of cultured fibroblasts and in extracellular matrix fibrils were colocalized by fluorescent and high voltage immunoelectron microscopy. Fibroblast cultures grown in the presence or absence of cycloheximide were incubated with exogenous plasma fibronectin labeled with fluorescein isothiocyanate. A monoclonal antibody specific for the EIIIA sequence of cellular fibronectin was used to detect cellular fibronectin. A rabbit antifluorescein antibody identified fluoresceinated plasma fibronectin. In cultures incubated in the presence of cycloheximide, plasma fibronectin was bound to the cell surface and was assembled into extracellular fibrils. In cultures grown in the absence of cycloheximide, plasma and cellular fibronectins were observed in the same matrix fibrils and in the same locations on the cell surface. There was not, however, random admixture of the two proteins.
Biological and clinical evidence for somatic mutations in BRCA1 and BRCA2 as predictive markers for olaparib response in high-grade serous ovarian cancers in the maintenance setting (2017)

To gain a better understanding of the role of somatic mutations in olaparib response, next-generation sequencing (NGS) of BRCA1 and BRCA2 was performed as part of a planned retrospective analysis of tumors from a randomized, double-blind, Phase II trial (Study 19; D0810C00019; NCT00753545) in 265 patients with platinum-sensitive high-grade serous ovarian cancer. BRCA1/2 loss-of-function mutations were found in 55% (114/209) of tumors, were mutually exclusive, and demonstrated high concordance with Sanger-sequenced germline mutations in matched blood samples, confirming the accuracy (97%) of tumor BRCA1/2 NGS testing. Additionally, NGS identified somatic mutations absent from germline testing in 10% (20/209) of the patients. Somatic mutations had >80% biallelic inactivation frequency and were predominantly clonal, suggesting that BRCA1/2 loss occurs early in the development of these cancers. Clinical outcomes between placebo- and olaparib-treated patients with somatic BRCA1/2 mutations were similar to those with germline BRCA1/2 mutations, indicating that patients with somatic BRCA1/2 mutations benefit from treatment with olaparib.
Enhanced wavelet based audio coder (2002)

Several enhancements on the wavelet based audio coder proposed by Sinha and Tewfik (see. Proceedings of the Int. Conf. of Acoust., Speech and Signal Proc. Society (ICASSP), vol.1, p.197-200, 1993) are discussed. These enhancements include use of temporal information in masking for audio segments with sudden change of amplitudes in them, efficient quantization of the variances and run-length coding for the zeros. Experiments suggest that these enhancements give almost transparent compression of monophonic CD quality audio signals (sampled at 44.1 kHz) at bit rates of 55-63 kbits/sec. We also discuss a real time implementation of a simplified version of the wavelet based audio coder at somewhat higher bit rates (64-78 kbits/sec).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Identification of candidate genes for human retinal degeneration loci using differentially expressed genes from mouse photoreceptor dystrophy models. (2008)

We have shown that this comparative genomics approach verified existing RD genes as well as identified novel RD candidate genes. The results on the characterization of the PLA2G7 protein, one of the novel RD genes, suggests that retinal tissue PLA2G7 levels may constitute an important risk factor for BCMAD. In summary, this reverse mapping approach, using accepted mouse models of human disease and known human RD loci, may prove useful in identifying possible novel disease candidates for RD and may be applicable to other human diseases.
High Open-Circuit Voltage Solar Cells Based on Organic–Inorganic Lead Bromide Perovskite (2013)

Mesoscopic solar cells, based on solution-processed organic-inorganic perovskite absorbers, are a promising avenue for converting solar to electrical energy. We used solution-processed organic-inorganic lead halide perovskite absorbers, in conjunction with organic hole conductors, to form high voltage solar cells. There is a dire need for low-cost cells of this type, to drive electrochemical reactions or as the high photon energy cell in a system with spectral splitting. These perovskite materials, although spin-coated from solution, form highly crystalline materials. Their simple synthesis, along with high chemical versatility, allows tuning their electronic and optical properties. By judicious selection of the perovskite lead halide-based absorber, matching organic hole conductor, and contacts, a cell with a ∼ 1.3 V open circuit voltage was made. While further study is needed, this achievement provides a general guideline for additional improvement of cell performance.
Bystander Tumoricidal Effect and Gap Junctional Communication in Lung Cancer Cell Lines (1998)

Tumor cells expressing the herpes simplex virus-thymidine kinase (HSV-tk) gene become sensitive to ganciclovir (GCV), and the phenomenon by which tumor cells surrounding the HSV-tk expressing cells also become sensitive to GCV is known as the "bystander effect." The purpose of this study was to investigate the bystander effect in human lung-cancer cell lines, and the role of gap-junctional intercellular communication as the mechanism responsible for it. Gap-junctional intercellular communication was measured both with a dye-transfer assay involving single-cell microinjection of Lucifer Yellow and with a PKH26/calcein-AM double-dye-transfer assay. Significant bystander tumoricidal effect was observed in lung-cancer cell lines when cultured cells contained only 10% HSV-tk expressing cells. This was also observed to occur with cell lines of different origin or from different species. Although gap-junctional intercellular communication characterized by rapid transfer of Lucifer Yellow was not observed, we did detect gap-junctional communication marked by the slow transfer of calcein-AM in lung-cancer cell lines. However, neither an inhibitor (1-octanol) nor an enhancer (all trans-retinoic acid [ATRA]) of gap-junctional communication affected the extent of the bystander effect. These findings suggest that low levels of gap-junctional communication may be efficient for producing the bystander effect in lung-cancer cells, or that other mechanisms may underlie this effect. Although gap-junctional communication may play an important role in generating the bystander effect in tumor cells expressing the HSV-tk gene, further knowledge of the mechanism of this effect may help improve the treatment of lung cancer with an HSV-tk system.
MicroRNA Expression Signature and Antisense-Mediated Depletion Reveal an Essential Role of MicroRNA in Vascular Neointimal Lesion Formation (2007)

MicroRNAs (miRNAs) are a recently discovered class of endogenous, small, noncoding RNAs that regulate about 30% of the encoding genes of the human genome. However, the role of miRNAs in vascular disease is currently completely unknown. Using microarray analysis, we demonstrated for the first time that miRNAs are aberrantly expressed in the vascular walls after balloon injury. The aberrantly expressed miRNAs were further confirmed by Northern blot and quantitative real-time polymerase chain reaction. Modulating an aberrantly overexpressed miRNA, miR-21, via antisense-mediated depletion (knock-down) had a significant negative effect on neointimal lesion formation. In vitro, the expression level of miR-21 in dedifferentiated vascular smooth muscle cells was significantly higher than that in fresh isolated differentiated cells. Depletion of miR-21 resulted in decreased cell proliferation and increased cell apoptosis in a dose-dependent manner. MiR-21-mediated cellular effects were further confirmed in vivo in balloon-injured rat carotid arteries. Western blot analysis demonstrated that PTEN and Bcl-2 were involved in miR-21-mediated cellular effects. The results suggest that miRNAs are novel regulatory RNAs for neointimal lesion formation. MiRNAs may be a new therapeutic target for proliferative vascular diseases such as atherosclerosis, postangioplasty restenosis, transplantation arteriopathy, and stroke.
Genome engineering of stem cell organoids for disease modeling (2017)

Precision medicine emerges as a new approach that takes into account individual variability. Successful realization of precision medicine requires disease models that are able to incorporate personalized disease information and recapitulate disease development processes at the molecular, cellular and organ levels. With recent development in stem cell field, a variety of tissue organoids can be derived from patient specific pluripotent stem cells and adult stem cells. In combination with the state-of-the-art genome editing tools, organoids can be further engineered to mimic disease-relevant genetic and epigenetic status of a patient. This has therefore enabled a rapid expansion of sophisticated in vitro disease models, offering a unique system for fundamental and biomedical research as well as the development of personalized medicine. Here we summarize some of the latest advances and future perspectives in engineering stem cell organoids for human disease modeling.
Can glutamine synthetase activity levels be modulated in transgenic plants by the use of recombinant DNA technology? (1994)

Conference Article| November 01 1994 Can glutamine synthetase activity levels be modulated in transgenic plants by the use of recombinant DNA technology? Stephen J. Temple; Stephen J. Temple * *To whom correspondence should be addressed. Search for other works by this author on: This Site PubMed Google Scholar Suman Bagga; Suman Bagga 1Plant Genetic Engineering Laboratories, Department of Agronomy and Horticulture, New Mexico State University, Las Cruces, MN 88003, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Champa Sengupta-Gopalan Champa Sengupta-Gopalan 1Plant Genetic Engineering Laboratories, Department of Agronomy and Horticulture, New Mexico State University, Las Cruces, MN 88003, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1994) 22 (4): 915–920. https://doi.org/10.1042/bst0220915 Article history Received: June 10 1994 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Stephen J. Temple, Suman Bagga, Champa Sengupta-Gopalan; Can glutamine synthetase activity levels be modulated in transgenic plants by the use of recombinant DNA technology?. Biochem Soc Trans 1 November 1994; 22 (4): 915–920. doi: https://doi.org/10.1042/bst0220915 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: AS, asparagine synthetase, CaMV, cauliflower mosaic virus, GDH, glutamate dehydrogenase, GOGAT, glutamate synthase, GS, glutamine synthetase © 1994 Biochemical Society1994 Article PDF first page preview Close Modal You do not currently have access to this content.
Inner Ear and Kidney Anomalies Caused by IAP Insertion in an Intron of the Eya1 Gene in a Mouse Model of BOR Syndrome (1999)

A spontaneous mutation causing deafness and circling behavior was discovered in a C3H/HeJ colony of mice at the Jackson Laboratory. Pathological analysis of mutant mice revealed gross morphological abnormalities of the inner ear, and also dysmorphic or missing kidneys. The deafness and abnormal behavior were shown to be inherited as an autosomal recessive trait and mapped to mouse chromosome 1 near the position of the Eya1 gene. The human homolog of this gene, EYA1, has been shown to underly branchio-oto-renal (BOR) syndrome, an autosomal dominant disorder characterized by hearing loss with associated branchial and renal anomalies. Molecular analysis of the Eya1 gene in mutant mice revealed the insertion of an intracisternal A particle (IAP) element in intron 7. The presence of the IAP insertion was associated with reduced expression of the normal Eya1 message and formation of additional aberrant transcripts. The hypomorphic nature of the mutation may explain its recessive inheritance, if protein levels in homozygotes, but not heterozygotes, are below a critical threshold needed for normal developmental function. The new mouse mutation is designated Eya1(bor) to denote its similarity to human BOR syndrome, and will provide a valuable model for studying mutant gene expression and etiology.
A Combinatorial Scaffold Approach toward Kinase-Directed Heterocycle Libraries (2002)

A novel strategy for efficient synthesis of various substituted heterocycles as kinase-directed combinatorial libraries is described. The general scheme involves capture of various dichloroheterocycles onto solid support and further elaborations by aromatic substitution with amines at elevated temperature or by anilines, boronic acids, and phenols via palladium-catalyzed cross-coupling reactions, thus the scaffold itself is transformed into a diversity element within the combinatorial scheme. Libraries consisting of discrete and highly diverse heterocyclic small molecules constructed with these chemistries are currently being evaluated in a variety of cell and protein-based assays.
Immunoglobulin structure and function: genetic control of antibody diversity. (1982)

Recent studies of the molecular biological characteristics of lymphoid cells have markedly increased our understanding of how millions of different antibodies can be synthesized by an individual mammal. In particular, studies have shown how antibody genes are arranged and rearranged within B-lymphocyte clones to provide each cell clone with antibody of defined specificity for antigen. The process involves the assembly, from disparate genetic elements, of a complete antibody gene that will code for an antibody protein. The assembly process, in itself, also provides mechanisms for generating the diversity of antibody variable region structure (that part of an antibody molecule that actually binds antigen) that is essential to a full role for humoral immunity in host defense mechanisms. Specifically, the diversity of structure characteristic of mature antibodies derives from 3 distinct mechanisms: innate variability of germ-line genes; mismatching of individual gene segments during their somatic rearrangement leading to junctional diversity; and somatic mutation in variable region genetic material during or after the rearrangement. Thus, it is now clearly understood that several processes are involved in explaining the origin of the antigen-combining diversity of antibody proteins. Certain "lottery-like" aspects of these genetic processes add to the combinatorial possibilities that are characteristic of the humoral immune system.
Designed Dendrimer Syntheses by Self-Assembly of Single-Site, ssDNA Functionalized Dendrons (2004)

Single site, functionalized, single stranded (ssDNA) dendri-poly(amidoamine) (PAMAM) di-dendrons have been synthesized by covalently conjugating complementary 32 base pair oligonucleotides to single-site, thiol functionalized dendri-PAMAM di-dendrons possessing neutral or anionic surface groups. Combining these complementary (ss-DNA) functionalized PAMAM di-dendrons at appropriate assembly temperatures produced Watson−Crick base paired (dsDNA) cores, surrounded by four PAMAM dendrons. These novel core−shell nanostructures represent a new class of precise monodisperse, linear-dendritic architectural copolymers. Using comparative gel electrophoresis, it was demonstrated that these self-assembled (di-dendron) dendrimers could be hemispherically differentiated as a function of surface chemistry as well as generational size. This new supramacromolecular approach offers a very facile and versatile strategy for the combinatorial design of size, shape, and surface substituents for both homogeneous and differentiated dendritic nanostructures.