gebhart/patrepeval huggingface dataset:
Engineering of Lactobacillus jensenii To Secrete RANTES and a CCR5 Antagonist Analogue as Live HIV-1 Blockers (2010)

The development of effective microbicides for the prevention of HIV-1 sexual transmission represents a primary goal for the control of AIDS epidemics worldwide. A promising strategy is the use of bacteria belonging to the vaginal microbiota as live microbicides for the topical production of HIV-1 inhibitors. We have engineered a human vaginal isolate of Lactobacillus jensenii to secrete the anti-HIV-1 chemokine RANTES, as well as C1C5 RANTES, a mutated analogue that acts as a CCR5 antagonist and therefore is devoid of proinflammatory activity. Full-length wild-type RANTES and C1C5 RANTES secreted by L. jensenii were purified to homogeneity and shown to adopt a correctly folded conformation. Both RANTES variants were shown to inhibit HIV-1 infection in CD4(+) T cells and macrophages, displaying strong activity against HIV-1 isolates of different genetic subtypes. This work provides proof of principle for the use of L. jensenii-produced C1C5 RANTES to block HIV-1 infection of CD4(+) T cells and macrophages, setting the basis for the development of a live anti-HIV-1 microbicide targeting CCR5 in an antagonistic manner.
Orf virus-encoded interleukin-10 inhibits maturation, antigen presentation and migration of murine dendritic cells (2003)

Orf virus (ORFV) belongs to the genus Parapoxvirus and induces cutaneous pustular lesions in sheep, goats and humans. ORFV is unusual in that it has the ability to reinfect its host and this suggests that the generation of immunological memory has been impaired, thus exposing the host to subsequent infection. The discovery that ORFV encodes an IL-10-like virokine raises the question of whether this factor adversely affects the cells that initiate the acquired immune response. We examined the effect of ORFV-IL-10 on immature murine bone marrow-derived dendritic cells (BMDC). Immature BMDC are activated on exposure to antigen and undergo maturation. This process is characterized by increased expression of CD80, CD86 and MHC class II and reduced antigen uptake. We found that the maturation of BMDC is impaired in cells treated with ORFV-IL-10 prior to antigen exposure and this was exemplified by the reduced expression of the cell-surface markers described above. We have also shown that the activation of a haemagglutinin peptide (HAT)-specific T cell hybridoma by dendritic cell-mediated presentation of HAT and heat-inactivated influenza virus AP8/34 was markedly reduced following exposure to ORFV-IL-10. Finally, we examined the effect of ORFV-IL-10 on Langerhans' cell (LC) migration using cultured murine skin explant tissue and showed that this virokine impaired the spontaneous migration of LC from the epidermis and induced changes in LC morphology. Our findings suggest that ORFV-IL-10 has the capacity to impair the initiation of an acquired immune response and hence inhibit the generation of immunological memory necessary for immunity on subsequent exposure.
Tearing based automatic abstraction for CTL model checking (1996)

In this paper we present the tearing paradigm as a way to automatically abstract behavior to obtain upper and lower bound approximations of a reactive system. We present algorithms that exploit the bounds to perform conservative ECTL and ACTL model checking. We also give an algorithm for false negative (or false positive) resolution for verification based on a theory of a lattice of approximations. We show that there exists a bipartition of the lattice set based on positive versus negative verification results. Our resolution methods are based on determining a pseudo-optimal shortest path from a given, possibly coarse but tractable approximation, to a nearest point on the contour separating one set of the bipartition from the other.
A-Myb Up-regulates Bcl-2 through a Cdx Binding Site in t(14;18) Lymphoma Cells (2000)

In follicular lymphoma, bcl-2 is translocated to the immunoglobulin heavy chain locus leading to deregulation of bcl-2 expression. We examined the role of Myb proteins in the regulation of bcl-2 expression in lymphoma cells. We showed that A-Myb up-regulates bcl-2 promoter activity. Northern and Western analyses demonstrated that A-Myb was expressed in the DHL-4 t(14;18) cell line. In t(14;18) cells and mature B cells, A-Myb up-regulated bcl-2 expression, whereas B- and c-Myb had little effect on bcl-2 gene expression. Deletion analysis of the bcl-2 5'-region identified a region responsive to A-Myb in t(14;18) cells. A potential binding site for the Cdx homeodomain proteins was located in this sequence. Analysis of the A-Myb-responsive region by UV cross-linking experiments revealed that a 32-kDa protein formed a complex with this region, but direct binding by Myb proteins could not be demonstrated. A-Myb could be recovered along with Cdx2 when nuclear extracts were passed over the Cdx site. Mutagenesis of the Cdx binding site abolished binding by the 32-kDa protein and significantly reduced the ability of A-Myb to induce bcl-2 expression. A strong induction of bcl-2 P2 promoter activity was observed in cotransfection studies of DHL-4 cells with the A-Myb and Cdx2 expression vectors, and increased endogenous Bcl-2 protein expression was observed in B cells transfected with A-Myb and/or Cdx2 expression constructs.
Effect of prosthetic ankle units on the gait of persons with bilateral trans-femoral amputations (2008)

In able-bodied individuals, the ankle joint functions to provide shock absorption, aid in foot clearance during the swing phase, and provides a rocker mechanism during stance phase to facilitate forward progression of the body. Prosthetic ankles currently used by persons with lower limb amputations provide considerably less function than their anatomical counterparts. However, increased ankle motion in the sagittal plane may improve the gait of persons with lower limb amputations while providing a more versatile prosthesis. The primary aim of this study was to examine and quantify temporal-spatial, kinematic, and kinetic changes in the gait of four male subjects with bilateral trans-femoral amputations who walked with and without prosthetic ankle units. Two prosthesis configurations were examined: (i) Baseline with only two Seattle LightFoot2 prosthetic feet, and (ii) with the addition of Endolite Multiflex Ankle units. Data from the gait analyses were compared between prosthetic configurations and with a control group of able-bodied subjects. The amputee subjects' freely-selected walking speeds, 0.74 +/- 0.19 m/s for the Baseline condition and 0.81 +/- 0.15 m/s with the ankle units, were much less than that of the control subjects (1.35 +/- 0.10 m/s). The amputee subjects demonstrated no difference in walking speed, step length, cadence, or ankle, knee, and hip joint moments and powers between the two prosthesis configurations. Sagittal plane ankle range of motion, however, increased by 3-8 degrees with the addition of the prosthetic ankle units. Compared to the control group, following initial contact the amputee subjects passively increased the rate of energy storage or dissipation at the prosthetic ankle joint, actively increased the power generation at the hip, and increased the extension moment at the hip while wearing the prosthetic ankle configuration. The amputee subjects increased the power generation at their hips, possibly as compensation for the reduced rate of energy return at their prosthetic ankles. Results from subject questionnaires administered following the gait analyses revealed that the prosthetic ankle units provided more comfort during gait and did not increase the perceived effort to walk. The subjects also indicated that they preferred walking with the prosthetic ankle units compared to the Baseline configuration. The results of the study showed that the prosthetic ankle units improved sagittal plane ankle range of motion and increased the comfort and functionality of the amputee subjects' prostheses by restoring a significant portion of the ankle rocker mechanism during stance phase. Therefore, prosthetic ankle mechanisms should be considered a worthwhile option when prostheses are prescribed for persons with trans-femoral amputations.
The 6-kilobase c-erbB2 promoter contains positive and negative regulatory elements functional in human mammary cell lines. (1994)

A 6-kilobase fragment extending at the 5'-end of the c-erbB2 protooncogene was isolated from a normal human lymphocyte genomic DNA library. The full-length fragment and five subfragments with identical 3'-ends were obtained by progressive unidirectional deletion from the 5'-end and were cloned in front of the luciferase reporter gene. The hybrid genes were analyzed for transcriptional activity in human mammary cell lines synthesizing low (HBL-100 and T-47D), moderate (MDA-MB-453), or high (BT-474) amounts of the c-erbB2 mRNA and were also analyzed in HeLa cells. Gene-specific expression was observed, indicating the presence of multiple cis-acting sequences in the c-erbB2 promoter. A major negative element is located in the -2- to -4-kilobase region. It is flanked on both sides by positive elements that display enhanced transcriptional activity in the BT-474 tumor cells only. While predominant in the low-expressing cells, the effect of the repressor appears to be overcome by the distal transactivator in the high-expressing BT-474 cells, resulting in a 15 to 50 times increase in luciferase activity relative to the HBL-100 and T-47D cells, respectively. Cell-specific expression relies on the trans-acting factors present in the different cell lines. The formation of cell-specific protein-DNA complexes was demonstrated by gel retardation assay.
Lack of effect of weak low frequency electromagnetic fields on chick embryogenesis. (1984)

Fertilised chicken eggs were incubated for 48 hours while exposed to pulsed trains of square wave magnetic fields having a duration of 0.5 msec and pulse repetition rates of 100 or 1000 Hz at magnetic field flux densities of 1.2 and 12 mu T. After exposure the embryos were scored blind for eight different gross structural features: primary vesicles, anterior neuropore, optic vesicles, auditory pits, truncal nervous system, heart, somites and blood vessels. There were no differences between exposed, sham-exposed and control eggs.
TiSGeD: a database for tissue-specific genes (2010)

http://bioinf.xmu.edu.cn/databases/TiSGeD/index.html.
Tattoo-Based Noninvasive Glucose Monitoring: A Proof-of-Concept Study (2014)

We present a proof-of-concept demonstration of an all-printed temporary tattoo-based glucose sensor for noninvasive glycemic monitoring. The sensor represents the first example of an easy-to-wear flexible tattoo-based epidermal diagnostic device combining reverse iontophoretic extraction of interstitial glucose and an enzyme-based amperometric biosensor. In-vitro studies reveal the tattoo sensor's linear response toward physiologically relevant glucose levels with negligible interferences from common coexisting electroactive species. The iontophoretic-biosensing tattoo platform is reduced to practice by applying the device on human subjects and monitoring variations in glycemic levels due to food consumption. Correlation of the sensor response with that of a commercial glucose meter underscores the promise of the tattoo sensor to detect glucose levels in a noninvasive fashion. Control on-body experiments demonstrate the importance of the reverse iontophoresis operation and validate the sensor specificity. This preliminary investigation indicates that the tattoo-based iontophoresis-sensor platform holds considerable promise for efficient diabetes management and can be extended toward noninvasive monitoring of other physiologically relevant analytes present in the interstitial fluid.
Self-Powered, Ultrasensitive, Flexible Tactile Sensors Based on Contact Electrification (2014)

Tactile/touch sensing is essential in developing human-machine interfacing and electronic skins for areas such as automation, security, and medical care. Here, we report a self-powered triboelectric sensor based on flexible thin-film materials. It relies on contact electrification to generate a voltage signal in response to a physical contact without using an external power supply. Enabled by the unique sensing mechanism and surface modification by polymer-nanowires, the triboelectric sensor shows an exceptional pressure sensitivity of 44 mV/Pa (0.09% Pa(-1)) and a maximum touch sensitivity of 1.1 V/Pa (2.3% Pa(-1)) in the extremely low-pressure region (<0.15 KPa). Through integration of the sensor with a signal-processing circuit, a complete tactile sensing system is further developed. Diverse applications of the system are demonstrated, explicitly indicating a variety of immediate uses in human-electronics interface, automatic control, surveillance, remote operation, and security systems.
Industry sizes up nanotubes (2000)

The numerous extraordinary properties of carbon nanotubes are now well known – as the other articles in this special issue emphasize – and it is clear that nanotubes differ from ordinary molecules and solids in many respects. In fact, nanotubes have an ambiguous identity: they have reasonably well defined structures – albeit a large variety of them – like molecules, but their relatively large length and width means that they also resemble solids. Indeed, nanotubes can be extended to macroscopic lengths and Widths to ultimately merge With bulk graphite, which has a familiar layered structure.
Intelligent browsing for multimedia applications (1996)

Describes a technique which we term "intelligent browsing"; the software built upon this technique assists the users in their search through a multimedia database. Searching for an item of interest in a large multimedia database is a complex task, even for users who have knowledge about it. An active (intelligent) browser suggests to the user items it estimates to be close to the target of the search. The intelligent part of the active browser consists of a learning module, whose object of study is the profile of the users' search actions. After a target is detected, the intelligent browser brings the item or items of interest on to the screen in a dedicated window, and controls the synchronization of the audio, video or written components of the item. If confirmed by the user, the dedicated window is transformed into a series of windows allocated to the desired item. The novel aspect of intelligent browsing is that it is entirely unobtrusive: it infers its similarity measure from the user's normal browsing actions, without any special input. Use of the browser on a multimedia application is illustrated in this paper.
The Massachusetts Electric Solar Project: a pilot project to commercialize residential PV systems (2002)

In February 1999, Massachusetts Electric Company (Mass Electric) announced a pilot photovoltaic commercialization project in the town of Medford, MA. Residential customers in that town were offered the opportunity to purchase a 250 W or 500 W grid-tied PV system for their home at a subsidized price. SunSine((R)) AC modules combine a state-of-the-art electronic inverter with a large, powerful PV module to simplify the grid-tied residential PV installations. The original project had a limit of 15 kW, which translates to 30-60 installations. The project is an on-going joint effort between Mass Electric and Ascension Technology, a Division of Applied Power Corporation. Sponsors also include the US DOE through the UPVG Team-UP program. This paper describes the project including system design, lessons learned and customer responses.
Regulation of eye development by frizzled signaling in Xenopus (2001)

Eye development in both invertebrates and vertebrates is regulated by a network of highly conserved transcription factors. However, it is not known what controls the expression of these factors to regulate early eye formation and whether transmembrane signaling events are involved. Here we establish a role for signaling via a member of the frizzled family of receptors in regulating early eye development. We show that overexpression of Xenopus frizzled 3 (Xfz3), a receptor expressed during normal eye development, functions cell autonomously to promote ectopic eye formation and can perturb endogenous eye development. Ectopic eyes obtained with Xfz3 overexpression have a laminar organization similar to that of endogenous eyes and contain differentiated retinal cell types. Ectopic eye formation is preceded by ectopic expression of transcription factors involved in early eye development, including Pax6, Rx, and Otx2. Conversely, targeted overexpression of a dominant-negative form of Xfz3 (Nxfz3), consisting of the soluble extracellular domain of the receptor, results in suppression of endogenous Pax6, Rx, and Otx2 expression and suppression of endogenous eye development. This effect can be rescued by coexpression of Xfz3. Finally, overexpression of Kermit, a protein that interacts with the C-terminal intracellular domain of Xfz3, also blocks endogenous eye development, suggesting that signaling through Xfz3 or a related receptor is required for normal eye development. In summary, we show that frizzled signaling is both necessary and sufficient to regulate eye development in Xenopus.
Conformation and Dynamics of DNA and Protein-DNA Complexes by 31P NMR (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConformation and Dynamics of DNA and Protein-DNA Complexes by 31P NMRDavid G. GorensteinCite this: Chem. Rev. 1994, 94, 5, 1315–1338Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://pubs.acs.org/doi/10.1021/cr00029a007https://doi.org/10.1021/cr00029a007research-articleACS PublicationsRequest reuse permissionsArticle Views1011Altmetric-Citations188LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Design of double-coated optical fibers to minimize long-term hydrostatic-pressure-induced microbending losses (2001)

The design of double-coated optical fibers to minimize long-term hydrostatic-pressure-induced microbending losses is investigated. Microbending loss in these fibers is dominated by compressive radial stress at the interface between the glass fiber and the primary coating, which is a function of the material properties of the polymeric coatings and their thickness. To minimize long-term hydrostatic-pressure-induced microbending losses, one should decrease the Young's modulus and Poisson ratio of the primary coating but increase the radius, Young's modulus, Poisson ratio, and relaxation time of the secondary coating. Alternatively, the radius and relaxation time of the primary coating have their optimum values.
A Novel Reduction of Zinc(II) Chloride with Samarium Metal and its Application to Silylation of 1-Alkynes (1996)

Samarium metal-induced reduction of zinc(II) chloride provides a convenient and efficient route to activated zinc, which allows us to silylate 1-alkynes with chlorosilanes effectively.
MICROWAVE ROTARY JOINTS FOR X-, C-, AND S-BAND (1965)

Abstract : This report consists of an examination of post-1950 rotary joint designs for use at X-, C-, and S-bands. Among the topics discussed are single and multichannel rotary joints with coaxial line or rectangular waveguide inputs. Since the simplest waveguide rotary joints incorporate transitions from rectangular to circular waveguide or from rectangular waveguide to a coaxial line, calculations are included on the relative power-handling capabilities of circular waveguide, rectangular waveguide, and coaxial line when subject to the conditions necessary for proper functioning of a rotary joint. A detailed examination is made of rectangular waveguide joints of the coaxial line, circular waveguide, coaxial mode converter, and of the annular varieties. It is shown that, in the circular waveguide variety, either a circularly polarized H(11) mode or an E(01) mode can be used. Methods of avoiding undesirable performance due to resonances in the circular waveguide are discussed. Methods are described of using circular waveguide rotary joints for dual-channel applications.
A Simple Estimate of Glomerular Filtration Rate in Children Derived From Body Length and Plasma Creatinine (1976)

Based on statistical analysis of data in 186 children, a formula was derived which allows accurate estimation of glomerular filtration rate (GFR) from plasma creatinine and body lenght (GFR(ml/min/1.73 sq m) = 0.55 length (cm)/Per (mg/dl). Its application to clearance data in a separate group of 223 children reveals excellent agreement with GFR estimated by the Ccr (r = .935) or Cin (r = .905). This formula should be useful for adjusting dosages of drugs excreted by the kidney and detecting significant changes in renal function.
Overexpression of G protein‐coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma (2012)

Overexpression in poor-risk myeloma, low expression in normal tissues and cell surface expression identify GPRC5D as a potential novel cancer antigen. Our data demonstrate that GPRC5D is a prognostic factor in MM correlating with other major risk factors.
Distinct roles of type I bone morphogenetic protein receptors in the formation and differentiation of cartilage (1997)

The bone morphogenetic proteins (BMPs), TGF beta superfamily members, play diverse roles in embryogenesis, but how the BMPs exert their action is unclear and how different BMP receptors (BMPRs) contribute to this process is not known. Here we demonstrate that the two type I BMPRs, BMPR-IA and BMPR-IB, regulate distinct processes during chick limb development. BmpR-IB expression in the embryonic limb prefigures the future cartilage primordium, and its activity is necessary for the initial steps of chondrogenesis. During later chondrogenesis, BmpR-IA is specifically expressed in prehypertrophic chondrocytes. BMPR-IA regulates chondrocyte differentiation, serving as a downstream mediator of Indian Hedgehog (IHH) function in both a local signaling loop and a longer-range relay system to PTHrP. BMPR-IB also regulates apoptosis: Expression of activated BMPR-IB results in increased cell death, and we showed previously that dominant-negative BMPR-IB inhibits apoptosis. Our studies indicate that in TGF beta signaling systems, different type I receptor isoforms are dedicated to specific functions during embryogenesis.