gebhart/patrepeval huggingface dataset:
[Expression and significance of aPKC-iota and E-cadherin in cholangiocarcinoma]. (2007)

The expression of aPKC-iota and E-cadherin may reflect the differentiation and invasive potential of cholangiocarcinoma. As a polar regulation-associated protein, aPKC-iota may play a role in the invasion and metastasis of cholangiocarcinoma.
Inhibitory Neural Activity Predicts Response to Cognitive-Behavioral Therapy for Posttraumatic Stress Disorder (2013)

anzctr.org Identifier: ACTRN12610000017022.
The human milk oligosaccharide disialyllacto-N-tetraose prevents necrotising enterocolitis in neonatal rats (2011)

HMO reduce NEC in neonatal rats and the effects are highly structure specific. If these results translate to NEC in humans, DSLNT could be used to prevent or treat NEC in formula-fed infants, and its concentration in the mother's milk could serve as a biomarker to identify breast-fed infants at risk of developing this disorder.
One‐Pot Synthesis of N‐Substituted β‐Amino Alcohols from Aldehydes and Isocyanides (2015)

A practical two-stage one-pot synthesis of N-substituted β-amino alcohols using aldehydes and isocyanides as starting materials has been developed. This method features mild reaction conditions, broad scope, and general tolerance of functional groups. Based on a less common central carbon-carbon bond disconnection, this protocol complements traditional approaches that involve amines and various carbon electrophiles (epoxides, α-halo ketones, β-halohydrins). Medicinally relevant products can be prepared in a concise and efficient way from simple building blocks, as demonstrated in the synthesis of the antiasthma drug salbutamol. Upgrading the synthesis to an enantioselective variant is also feasible.
Effects of External Irradiation on the Thyroid Gland in Childhood (1988)

Review Articles| December 02 2008 Effects of External Irradiation on the Thyroid Gland in Childhood Subject Area: Endocrinology , Women's and Children's Health N.D. Barnes N.D. Barnes Addenbrookes Hospital, Cambridge, UK Search for other works by this author on: This Site PubMed Google Scholar Hormone Research (1988) 30 (2-3): 84–89. https://doi.org/10.1159/000181034 Article history Published Online: December 02 2008 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation N.D. Barnes; Effects of External Irradiation on the Thyroid Gland in Childhood. Hormone Research 31 December 1988; 30 (2-3): 84–89. https://doi.org/10.1159/000181034 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsHormone Research in Paediatrics Search Advanced Search Keywords: External irradiation, Thyroid, Childhood This content is only available via PDF. 1988Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.
Opiate Receptor: Demonstration in Nervous Tissue (1973)

Tritiated naloxone, a powerful opiate antagonist, specifically binds to an opiate receptor of mammalian brain and guinea pig intestine. Competition for the opiate receptor by various opiates and their antagonists closely parallels their pharmacological potency. The opiate receptor is confined to nervous tissue.
Bifidobacterium infantisstrains with and without a combination of Oligofructose and Inulin (OFI) attenuate inflammation in DSS-induced colitis in rats (2006)

Administrations of OFI and Bifidobacterium improve DSS-induced acute colitis and have an anti-inflammatory effect. Major differences in effect were observed between the two B. infantis strains as indicated in MDA and succinic acid concentration as well as bacterial translocation rate in synbiotic combinations.
Combined passive and active immunization for interruption of perinatal transmission of hepatitis B virus in Taiwan. (1985)

We attempted a clinical trial to interrupt transmission of hepatitis B virus (HBV) infection from hepatitis B surface antigen (HBsAg) positive and hepatitis Be antigen (HBsAg) positive mothers to their infants in Taiwan. Screening of 5,595 pregnant women revealed that 856 (15.3%) were HBsAg positive. Three hundred and sixty-one (42.2%) of the HBsAg positive pregnant women were HBeAg positive. Infants born to HBsAg and HBeAg positive mothers were randomized into 3 groups to receive the HBV vaccine alone or combined with hepatitis B immune globulin (HBIG). HGV vaccine was given at 2, 6, and 10 weeks after birth. Group I received HBV vaccine alone while Group II received HBV vaccine in combination with HBIG at birth and group III received HBV vaccine plus HBIG at birth and again at one month old. Group IV constituted the control group when their parents refused vaccination. At 6 months of age, the HBV carrier rate was 23.7% (9/38) in Group I, 11.1% (4/36) in Group II, and 5.3% (2/38) in Group III infants. Compared with 90% of infants who became HBV carriers in the control group (Group IV), the efficacy of HBV vaccination in preventing HBV infection among these high risk infants at the 6th month was 73.7% in Group I, 87.7% in Group II, and 94.1% in Group III. The antibody to HBsAg (anti-HBs) positivity rate in sera of Group I, II, III infants at 6 months of age was 79.0%, 88.9% and 94.7%, respectively. These initial results indicate that combined passive and active immunization is efficacious in interrupting perinatal transmission of HBV infection.
Measurement of Functional Activities in Older Adults in the Community (1982)

Two measures of social function designed for community studies of normal aging and mild senile dementia were evaluated in 195 older adults who underwent neurological, cognitive, and affective assessment. An examining and a reviewing neurologist and a neurologically trained nurse independently rated each on a Scale of Functional Capacity. Interrater reliability was high (examining vs. reviewing neurologist, r = .97; examining neurologist vs. nurse, tau b = .802; p less than .001 for both comparisons). Estimates correlated well with an established measure of social function and with results of cognitive tests. Alternate informants evaluated participants on the Functional Activities Questionnaire and the Instrumental Activities of Daily Living Scale. The Functional Activities Questionnaire was superior to the Instrumental Activities of Daily scores. Used alone as a diagnostic tool, the Functional Activities Questionnaire was more sensitive than distinguishing between normal and demented individuals.
Convolutional Two-Stream Network Fusion for Video Action Recognition (2016)

Recent applications of Convolutional Neural Networks (ConvNets) for human action recognition in videos have proposed different solutions for incorporating the appearance and motion information. We study a number of ways of fusing ConvNet towers both spatially and temporally in order to best take advantage of this spatio-temporal information. We make the following findings: (i) that rather than fusing at the softmax layer, a spatial and temporal network can be fused at a convolution layer without loss of performance, but with a substantial saving in parameters, (ii) that it is better to fuse such networks spatially at the last convolutional layer than earlier, and that additionally fusing at the class prediction layer can boost accuracy, finally (iii) that pooling of abstract convolutional features over spatiotemporal neighbourhoods further boosts performance. Based on these studies we propose a new ConvNet architecture for spatiotemporal fusion of video snippets, and evaluate its performance on standard benchmarks where this architecture achieves state-of-the-art results.
Neuronal IGF‐1 resistance reduces Aβ accumulation and protects against premature death in a model of Alzheimer's disease (2009)

Alzheimer's disease (AD) is characterized by progressive neurodegeneration leading to loss of cognitive abilities and ultimately to death. Postmortem investigations revealed decreased expression of cerebral insulin-like growth factor (IGF)-1 receptor (IGF-1R) and insulin receptor substrate (IRS) proteins in patients with AD. To elucidate the role of insulin/IGF-1 signaling in AD, we crossed mice expressing the Swedish mutation of amyloid precursor protein (APP(SW), Tg2576 mice) as a model for AD with mice deficient for either IRS-2, neuronal IGF-1R (nIGF-1R(-/-)), or neuronal insulin receptor (nIR(-/-)), and analyzed survival, glucose, and APP metabolism. In the present study, we show that IRS-2 deficiency in Tg2576 mice completely reverses premature mortality in Tg2576 females and delays beta-amyloid (Abeta) accumulation. Analysis of APP metabolism suggested that delayed Abeta accumulation resulted from decreased APP processing. To delineate the upstream signal responsible for IRS-2-mediated disease protection, we analyzed mice with nIGF-1R or nIR deficiency predominantly in the hippocampus. Interestingly, both male and female nIGF-1R(-/-)Tg2576 mice were protected from premature death in the presence of decreased Abeta accumulation specifically in the hippocampus formation. However, neuronal IR deletion had no influence on lethality of Tg2576 mice. Thus, impaired IGF-1/IRS-2 signaling prevents premature death and delays amyloid accumulation in a model of AD.
Biosynthesis of Heparin (1973)

Abstract Microsomal fraction from mouse mastocytoma was incubated for 60 min with UDP-[14C]glucuronic acid and unlabeled UDP-N-acetylglucosamine, producing nonsulfated labeled polysaccharide. Continued incubation for an additional 60-min period, in the presence of 3'-phosphoadenylylsulfate, yielded sulfated, heparin-like polymer. The incorporation of 14C was terminated at the beginning of the sulfation period, by including an excess of unlabeled UDP-glucuronic acid in the incubation mixtures. The distribution in the nonsulfated and chase-sulfated 14C-polysaccharides, respectively, of N-unsubstituted, N-acetylated, and N-sulfated glucosamine residues was investigated by selective deamination with nitrous acid. Samples were treated at room temperature with 0.24 m NaNO2 in 1.8 m acetic acid for 80 min (Reaction A) or with 3.9 m NaNO2 in 0.28 m acetic acid for 10 min (Reaction B). In Reaction A the glycosidic bonds of glucosamine residues having either unsubstituted or sulfated amino groups were cleaved, whereas in Reaction B N-unsubstituted glucosamine units only were attacked. The effect of deamination on the labeled polysaccharide preparations was studied by gel chromatography of the products, on Sephadex G-25. Characterization of the nonsulfated polysaccharide, by use of these methods, indicated that about half of the glucosamine residues had unsubstituted amino groups. This result was confirmed by ion exchange chromatography, on DEAE-cellulose, which showed the presence of a polymer with less polyanion character than standard hyaluronic acid. After N-acetylation, the elution pattern of this intermediary polysaccharide was the same as that of hyaluronic acid. The sulfated polysaccharide contained N-sulfated and N-acetylated but no N-unsubstituted glucosamine units. It is concluded that N-deacetylation of the heparin precursor polysaccharide occurs in the absence of 3'-phosphoadenylylsulfate and that the amino groups thus exposed can serve as acceptors of sulfate residues.
A Cherry Protein and Its Gene, Abundantly Expressed in Ripening Fruit, Have Been Identified as Thaumatin-Like (1996)

A 29-kD polypeptide is the most abundant soluble protein in ripe cherry fruit (Prunus avium L); accumulation begins at the onset of ripening as the fruit turns from yellow to red. This protein was extracted from ripe cherries and purified by size-exclusion and ion-exchange chromatography. Antibodies to the purified protein were used to screen a cDNA library from ripe cherries. Numerous recombinant plaques reacted positively with the antibodies; the DNA sequence of representative clones encoded a polypeptide of 245 amino acid residues. A signal peptide was indicated, and the predicted mature protein corresponded to the purified protein in size (23.3 kD, by mass spectrometry) and isoelectric point (4.2). A search of known protein sequences revealed a strong similarity between this polypeptide and the thaumatin family of pathogenesis-related proteins. The cherry thaumatin-like protein does not have a sweet taste, and no antifungal activity was seen in preliminary assays. Expression of the protein appears to be regulated at the gene level, with mRNA levels at their highest in the ripe fruit.
Massive ex vivo generation of functional dendritic cells from mobilized CD34+ blood progenitors for anticancer therapy. (1995)

We report that blood cell autografts, collected by single leukapheresis in cancer patients (n = 11) at the time of mobilization of hematopoietic progenitors into peripheral blood following anticancer therapy with high-dose cyclophosphamide (HD-CTX) plus interleukin-3 (IL-3) and granulocyte colony-stimulating factor (G-CSF/filgrastim), comprise 1.98 +/- 0.39 x 10(5)/kg (mean +/- SE) CD34+ progenitors of dendritic cells (DCs). This number corresponds to 140-fold more progenitors than in a control autograft collected in the steady state. DCs derived from mobilized CD34+ cells, morphologically and immunophenotypically undistinguishable from skin Langerhans cells and DCs from bone marrow and cord blood CD34+ cells, are shown to be powerful stimulators of allogeneic T cell proliferation in primary MLR and of autologous HLA-DR-restricted CD4+ T cell proliferation in response to presentation of xenogenic antigens. We show that the GM-CSF-plus-TNF-alpha-dependent ex vivo generation of DCs from mobilized CD34+ cells is 2.5-fold enhanced by flk-2/flt-3 ligand or c-kit ligand (stem cell factor) and five-fold enhanced by a combination of these growth factors. In addition, the optimal serum for the generation of DCs is autologous HD-CTX recovery-phase serum rather than fetal calf serum (FCS) or steady-state human serum, which are clinically inadequate and ineffective, respectively. In practice, the stimulation of CD34+ cells in a blood cell autograft (15.75 +/- 2.46 x 10(6)/kg) provided by the above four growth factors should permit ex vivo generation of approximately 40 x 10(9) DCs in an adult patient. These new findings provide advantageous tools for the large-scale generation of DCs that are potentially usable for clinical protocols of immunotherapy or vaccination in patients undergoing cancer treatment.
Irinotecan in combination with new agents (2004)

Today, irinotecan in combination with bolus and/or infusional 5-FU/FA constitutes a standard first-line treatment for patients with metastatic colorectal cancer. In an attempt to further improve clinical outcome, the use of irinotecan in combination with novel, targeted agents has been investigated. The theoretical attraction of combining irinotecan with targeted therapies is that this can improve the efficacy of treatment, but, due to the mainly non-overlapping toxicities of the agents involved, the toxicity of treatment should not be exacerbated. In this article we discuss recent data from clinical studies looking at the combinations of irinotecan with the 5-fluorouracil (5-FU) pro-drug, capecitabine, the cyclo-oxygenase (COX-2) inhibitor, celecoxib, and monoclonal antibodies against the epidermal growth factor receptor (cetuximab) and vascular endothelial growth factor (bevacizumab).
High-mannose glycans on the Fc region of therapeutic IgG antibodies increase serum clearance in humans (2011)

Glycan structures attached to the C(H)2 domain of the Fc region of immunoglobulin G (IgG) are essential for specific effector functions but their role in modulating clearance is less clear. Clearance is of obvious importance for therapeutic monoclonal antibodies (Mabs) as it directly impacts efficacy. Here, we study the impact of Fc glycan structure on the clearance of four therapeutic human IgGs (one IgG1 and three IgG2s) in humans. The therapeutic IgGs were affinity purified from serum samples from human pharmacokinetic studies, and changes to the glycan profile over time were determined by peptide mapping employing high-resolution mass spectrometry. Relative levels of high-mannose 5 (M5) glycan decreased as a function of circulation time, whereas other glycans remained constant. These results demonstrate that therapeutic IgGs containing Fc high-mannose glycans are cleared more rapidly in humans than other glycan forms. The quantitative effect of this on pharmacokinetic area under the curve was calculated and shown to be relatively minor for three of the four molecules studied, but, depending on the dosing regimen and the relative level of the high-mannose glycan, this can also have significant impact. High-mannose content of therapeutic Mabs should be considered an important product quality attribute which may affect pharmacokinetic properties of therapeutic antibodies.
Changes in fibroglandular volume and water content of breast tissue during the menstrual cycle observed by MR imaging at 1.5 t (1995)

MR imaging at 1.5 T was used to investigate variations in breast parenchyma during the menstrual cycle. Seven subjects were examined twice weekly over at least one menstrual cycle. A three-point Dixon technique (TE = 19 msec, TR = 2000 msec) provided images of fat, water, and static magnetic field (Bo), from which two quantitative whole breast parameters were calculated: the mean relative volumetric water content, <WC>, and the mean volumetric fibroglandular fraction, <FV>. Four of seven subjects showed unequivocal cyclic variations in <WC> and <FV> consistent with expected histologic changes; <WC> and <FV> values were elevated during menses and reduced in mid-cycle. The maximum deviation measured for each of the four subjects was < or = 10% in <WC> and <FV> units. These variations probably do not influence significantly the clinical interpretation of unenhanced MR breast images. Quantitative measurements of breast parenchyma, however, should recognize these effects.
Early Clinical PET Imaging Results with the Novel PHF-Tau Radioligand [F18]-T808 (2013)

Aggregates of hyperphosphorylated tau (PHF-tau), such as neurofibrillary tangles, are linked to the degree of cognitive impairment in Alzheimer's disease. We have recently reported early clinical results of a novel PHF-tau targeting PET imaging agent, [F18]-T807. Since then, we have investigated a second novel PHF-tau targeting PET imaging agent, [F18]-T808, with different pharmacokinetic characteristics, which may be favorable for imaging Alzheimer's disease and other tauopathies. Here, we describe the first human brain images with [F18]-T808.
Direct current high-pressure glow discharges (1999)

Stabilization and control of a high-pressure glow discharge by means of a microhollow cathode discharge has been demonstrated. The microhollow cathode discharge, which is sustained between two closely spaced electrodes with openings of approximately 100 μm diam, serves as plasma cathode for the high-pressure glow. Small variations in the microhollow cathode discharge voltage generate large variations in the microhollow cathode discharge current and consequently in the glow discharge current. In this mode of operation the electrical characteristic of this system of coupled discharges resembles that of a vacuum triode. Using the microhollow cathode discharge as plasma cathode it was possible to generate stable, direct current discharges in argon up to atmospheric pressure, with estimated electron densities in the range from 1011 to 1012 cm−3. The recently demonstrated parallel operation of these discharges indicates the potential of this technique for the generation of large volume plasmas at high gas pressure through superposition of individual glow discharges.
miR-335 orchestrates cell proliferation, migration and differentiation in human mesenchymal stem cells (2010)

In spite of the extensive potential of human mesenchymal stem cells (hMSCs) in cell therapy, little is known about the molecular mechanisms that regulate their therapeutic properties. We aimed to identify microRNAs (miRNAs) involved in controlling the transition between the resting and reparative phenotypes of hMSCs, hypothesizing that these miRNAs must be present in the undifferentiated cells and downregulated to allow initiation of distinct activation/differentiation programs. Differential miRNA expression analyses revealed that miR-335 is significantly downregulated upon hMSC differentiation. In addition, hMSCs derived from a variety of tissues express miR-335 at a higher level than human skin fibroblasts, and overexpression of miR-335 in hMSCs inhibited their proliferation and migration, as well as their osteogenic and adipogenic potential. Expression of miR-335 in hMSCs was upregulated by the canonical Wnt signaling pathway, a positive regulator of MSC self-renewal, and downregulated by interferon-γ (IFN-γ), a pro-inflammatory cytokine that has an important role in activating the immunomodulatory properties of hMSCs. Differential gene expression analyses, in combination with computational searches, defined a cluster of 62 putative target genes for miR-335 in hMSCs. Western blot and 3'UTR reporter assays confirmed RUNX2 as a direct target of miR-335 in hMSCs. These results strongly suggest that miR-335 downregulation is critical for the acquisition of reparative MSC phenotypes.