gebhart/patrepeval huggingface dataset:
Identification of a Membrane-associated Inhibitor(s) of Epidermal Growth Factor-induced Signal Transducer and Activator of Transcription Activation (1998)

Many growth factors, including epidermal growth factor (EGF), can activate the signal transducer and activator of transcription (STAT) signaling pathway. Here, we demonstrate that STAT activation by EGF treatment is conditional. EGF activates STAT1 and STAT3 in A431 but not in HeLa and PC12 cells. Using a reconstituted in vitro STAT activation system, we have identified and partially purified a potential inhibitor (s) that is membrane-associated and can block STAT activation induced by EGF in vitro. However, this inhibitor has no effect on STAT complexes after they are formed. We have further shown that this inhibitor(s) also exists in many other cancer cell lines, suggesting that blocking the STAT activation during growth factor signal transduction may play a significant role in the development of many kinds of cancers.
Radiographic Comparison of Anterior Acetabular Rim Morphology Between Pincer Femoroacetabular Impingement and Control (2015)

Level III, retrospective comparative study.
Biotechnological and chemical routes to intermediate synthesis products from sucrose. (1990)

Des travaux au sujet de la biotechnologie et de la chimie du saccharose ont comme but des modifications selectives permettant d'obtenir des produits intermediaires pour la synthese de produits trouvant plus facilement une application industrielle. Une oxydation tres specifique du saccharose, du platinose ou d'autres disaccharides peut se faire au moyen de l'agrobacterium tumefaciens. On a ameliore sensiblement les rendements. La synthese chimique qui s'y rapporte, aussi celle de derives du saccharose comme le palatinose, donnait des derives mono- et bi-fonctionnels. Au moyen d'une amination en milieu reducteur on obtient les amines correspondants. L'enzyme dextransucrase, specifique au saccharose, permet de produire des oligosaccharides adaptes au besoin. Par exemple pour le trisaccharide panose on a elabore des voies vers une optimisation des rendements. On peut observer un large spectre de possibilites de synthese et d'applications
Syngeneic transfer of autoimmune diabetes from diabetic NOD mice to healthy neonates. Requirement for both L3T4+ and Lyt-2+ T cells. (1987)

We have developed a model of syngeneic adoptive transfer for type I diabetes mellitus of NOD mice. This model consists in injecting spleen cells from diabetic adult mice into newborn NOD recipients. 50% of recipients inoculated with 20 X 10(6) cells develop diabetes within the first 10 wk of life, at a time when none of the control littermates have yet become diabetic. The earliest successful transfers are observed at 3 wk of age, at a time when controls do not even exhibit histological changes in their pancreas. In addition we have shown that: (a) both males and females can be adoptively transferred, despite the fact that males rarely develop spontaneous diabetes in our colony; (b) diabetes transfer is a dose-dependent phenomenon that provides an in vivo assay for comparing the autoimmune potential of spleen cells from mice at various stages of their natural history; (c) the susceptibility of the recipients to the transfer is limited in time and declines after 3 wk; and (d) both L3T4+ and Lyt-2+ T cell subsets are necessary for the successful transfer. The neonatal syngeneic transfer provides an effective model for studies of the cellular events involved at regulatory and effector stages of autoimmune type I diabetes.
Use of a monoclonal antibody to detect DNA damage caused by the anticancer drug cis‐diamminedichloroplatinum (II) in vivo and in vitro (1994)

A monoclonal antibody, MAb62-5, was prepared and used to detect DNA damage due to the anticancer drug cis-diamminedichloroplatinum (II) (or cisplatin). ELISA competition indicated that the binding of MAb62-5 to cisplatin-DNA was competitively inhibited (50% control) by 210 nM of cisplatin bound to DNA, cisplatin/nucleotide (D/N) = 0.2. Using a DNA mobility shift assay, MAb62-5 binding activity was inhibited by 50% by approximately 50-fold molar excess of cisplatin-DNA adducts (D/N = 0.08), whereas there was less than 5% inhibition by UV-DNA adducts or mock-treated DNA. In addition, MAb62-5 showed a similar affinity to the cisplatin-DNA adducts as compared to an endogenous cisplatin-damaged DNA recognition protein. Using ELISA with this antibody, we have demonstrated a 2-fold enhancement in excision repair of cisplatin-DNA adducts in resistant HeLa cells. This is supported by the measurement of repair-associated DNA strand breaks using alkaline elution and host cell reactivation of transfected plasmid DNA carrying cisplatin damage. These findings also provide explanation for the complexicity of immunoassay in cells.
Posibilidades de dispensa jurisdiccional ¿tutela penal?: comercialización de las participaciones preferentes (2013)

En este trabajo se aborda el analisis de las participaciones preferentes, producto complejo, de caracter perpetuo, de alto riesgo, de rentabilidad no garantizada, comercializado por entidades financieras a clientes minoristas, de perfil conservador, que asola como un autentico tsunami al haber atrapado a miles de afectados, llegandose incluso a calificar como el «timo» de las preferentes.
OFF-State leakage current mechanisms in bulkSi and SOI MOSFETs and their impact on CMOS ULSIs standby current (2001)

In this work, an analytical model of the OFF-state leakage current in metal-oxide-semiconductor (MOS) transistors and its relation with the standby current of logic complementary MOS (CMOS) ICs is presented. The OFF leakage currents in (1) bulk MOS; (2) floating-body silicon-on-insulator (FB-SOI); and (3) body-tied SOI (BT-SOI) MOS field effect transistors (MOSFETs) are modeled based on physics mechanisms, equivalent circuits and operational block diagrams. Good correlation is obtained between the theory and experimental devices. The model clarifies and quantifies that in thin film FB-SOI, the OFF leakage current is dominated by (a) V/sub th/ at drain voltages lower than the onset of the "kink" effect V/sub ds/<V/sub dk/, and (b) the impact ionization-induced floating-body effect for V/sub ds/>V/sub dk/. The OFF-state leakage current model is successfully applied to the analysis and prediction of the leakage current components in logic integrated circuits (CMOS ICs).
Both v-Ha-Ras and v-Raf Stimulate Expression of the Vascular Endothelial Growth Factor in NIH 3T3 Cells (1995)

Stimulation of NIH 3T3 cells with platelet-derived growth factor (PDGF)-BB and 12-O-tetradecanoylphorbol-13-acetate (TPA) enhances vascular endothelial growth factor (VEGF) gene expression. To address the question of whether Ras and Raf are involved in the induction of VEGF gene expression by PDGF and TPA, we examined the effects of both factors on NIH 3T3 cells stably transfected with v-Ha-ras or v-raf. In serum-starved NIH 3T3 cells, only low levels of mRNA expression can be detected, whereas both ras and raf transformed cell lines express enhanced levels of a 4.3-kilobase VEGF transcript. Stimulation with PDGF or TPA resulted in increased VEGF mRNA in all cell lines, with highest levels found in the transformed cells. Immunofluorescence studies confirmed that the elevated VEGF mRNA expression correlated with enhanced protein levels. Positive immunofluorescence signals could be detected in v-Ha-ras or v-raf transformed cell lines but not in unstimulated NIH 3T3 cells. VEGF from conditioned medium of v-raf transformed NIH 3T3 cells was partially purified by chromatography on heparin-Sepharose. Biological activity of this VEGF protein was demonstrated by competition with binding of recombinant 125I-VEGF165 to human umbilical vein endothelial cells and by its ability to stimulate proliferation of these cells.
Identifying high-affinity aptamer ligands with defined cross-reactivity using high-throughput guided systematic evolution of ligands by exponential enrichment (2015)

Oligonucleotide aptamers represent a novel platform for creating ligands with desired specificity, and they offer many potentially significant advantages over monoclonal antibodies in terms of feasibility, cost, and clinical applicability. However, the isolation of high-affinity aptamer ligands from random oligonucleotide pools has been challenging. Although high-throughput sequencing (HTS) promises to significantly facilitate systematic evolution of ligands by exponential enrichment (SELEX) analysis, the enormous datasets generated in the process pose new challenges for identifying those rare, high-affinity aptamers present in a given pool. We show that emulsion PCR preserves library diversity, preventing the loss of rare high-affinity aptamers that are difficult to amplify. We also demonstrate the importance of using reference targets to eliminate binding candidates with reduced specificity. Using a combination of bioinformatics and functional analyses, we show that the rate of amplification is more predictive than prevalence with respect to binding affinity and that the mutational landscape within a cluster of related aptamers can guide the identification of high-affinity aptamer ligands. Finally, we demonstrate the power of this selection process for identifying cross-species aptamers that can bind human receptors and cross-react with their murine orthologs.
Induction of the metastatic phenotype by transfection of a benign rat mammary epithelial cell line with the gene for p9Ka, a rat calcium-binding protein, but not with the oncogene EJ-ras-1. (1993)

The rat mammary epithelial cell line, Rama 37, yields benign, non-metastasizing adenomatous tumours in syngeneic Wistar-Furth rats. Transfection of this line with a drug resistance plasmid containing both the gene for resistance to Geneticin (neo) and the gene for p9Ka (pSV2neo-p9Ka), a rat calcium-binding protein, or with a similar plasmid containing neo and the oncogene EJ-ras-1 (pSV2neo-ras) yields drug-resistant transformants that express high levels of the p9Ka or EJ-ras-1 mRNAs and proteins. These transfected cells all produce tumours when injected at subcutaneous sites with a shorter median latent period than the tumours produced by the parental untransfected Rama 37 cells in syngeneic hosts. Cells transfected with pSV2neo-p9Ka yield a higher incidence of tumours than untransfected Rama 37 cells, many of which metastasize to lungs and/or lymph nodes in syngeneic rats. However, cells transfected with pSV2-neo-ras or pSV2neo plasmid alone yield tumours that fail to metastasize. Immunofluorescent studies suggest an association of p9Ka with the cytoskeleton, as depicted by F-actin staining with the reagent phalloidin. It is suggested that the transfection of copies of the gene for the rat calcium-binding protein p9Ka can enhance the tumorigenic potential and induce the metastatic phenotype in this rat mammary model, whereas transfection of control plasmid DNA or the oncogene EJ-ras-1 fails to induce the metastatic phenotype, although EJ-ras-1 transfectants, like those containing p9Ka, possess increased growth properties in vivo.
Long-chain polystyrene-grafted polyethylene film matrix: a new support for solid-phase peptide synthesis (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLong-chain polystyrene-grafted polyethylene film matrix: a new support for solid-phase peptide synthesisRolf H. Berg, Kristoffer Almdal, Walther Batsberg Pedersen, Arne Holm, James P. Tam, and R. B. MerrifieldCite this: J. Am. Chem. Soc. 1989, 111, 20, 8024–8026Publication Date (Print):September 1, 1989Publication History Published online1 May 2002Published inissue 1 September 1989https://pubs.acs.org/doi/10.1021/ja00202a059https://doi.org/10.1021/ja00202a059research-articleACS PublicationsRequest reuse permissionsArticle Views347Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Recent Advances in Catalytic Asymmetric Addition to Imines and Related C=N Systems (2005)

Addition reactions to imines are relatively less developed compared to those of carbonyl compounds due to low electrophilicity and ease of α-deprotonation. With the use of an appropriate activator, which can coordinate to the imine nitrogen atom, the electrophilicity of the imine can be enhanced and a range of nucleophilic addition reactions becomes possible. When the activator is chiral, it will also create chiral environment and will direct the approach of the nucleophile to one face of the imine over the other resulting in enantioselectivity. The potential of catalytic asymmetric addition to imines in organic synthesis is enormous, but is relatively underused. With the development of many highly effective chiral catalysts for imine additions, the situation will soon be changed. The present review will cover selected literature on catalytic asymmetric additions to aldimines, ketimines and related compounds including hydrazones, oximes and nitrones from 1999 to 2004. The reactions of interest include hydrogenation, alkylation, Mannich and related reactions such as aza-Baylis-Hillman and aza-Henry reaction, Strecker reaction, hydrophosphonylation, and 3-6 membered ring forming reactions from imines. Some applications of these methodologies in synthesis of nitrogen-containing biologically active compounds will also be presented. Keywords: nucleophilic addition, chiral auxiliary, tautomerization, hydrogenation, hydride transfer reactions, hydrosilylation, alkyllithium reagents, alkylzinc reagents
Music Rx: Physiological and Emotional Responses to Taped Music Programs of Preoperative Patients Awaiting Plastic Surgery (1987)

This study investigated the physiological and emotional responses of patients awaiting an elective plastic surgery procedure to a 30-minute taped music program. The study replicated in part the research study of Bonny entitled "Music Rx." Values for the physiological variables of systolic blood pressure, diastolic blood pressure, pulse rate, mean arterial pressure (MAP), and double product index (DPI) were obtained before and after the music listening. These data were analyzed via a repeated measures t-test using each subject as her or his own control. The convenience sample of 10 was nonrandomized. Emotional responses were evaluated by means of an open-ended, nondirective questionnaire developed around 5 categories of depression, sadness, and despair; psychological isolation and defensiveness; anxiety; difficulty of medical management; and preoccupation with pain. Process recordings and documented verbal and body language were used before and after the music to identify themes and mood states expressed by patients. Every physiological variable decreased in value at the less than .001 level of significance. The most significant emotional effect appeared to be an experienced shift in patients' awareness toward a more relaxed, calm state. The most critical conclusion is that music listening appeared to effect desirable pattern shifts in physiological and emotional states in the presurgical setting for those patients studied.
Targeting cytotoxic T lymphocytes for cancer immunotherapy (2004)

In light of their preeminent role in cellular immunity, there is considerable interest in targeting of cytotoxic T-lymphocytes to cancer. This review summarises the active and passive immunotherapeutic approaches under development to achieve this goal, emphasising how recent advances in tumour immunology and gene transfer have impacted upon this field.
Third-generation and beyond (3.5G) wireless networks and its applications (2003)

This paper first describes the applications of 3G and beyond wireless networks and general trends. To address these advanced applications, WCDMA HSDPA promises to provide services of up to 10 Mbit/s and higher spectral efficiency for packet data. It represents a major evolution of the air interface since 1997 when the concept of 3G was adopted. We call it "3.5G" in this paper to differentiate the fundamental difference between 3G and 4G. For 4G, it is believed that the air interface of the 4G wireless network will be different from that of the 3G. The basic concept and principles of WCDMA HASDPA are discussed in the paper.
Progesterone-specific stimulation of triglyceride biosynthesis in a breast cancer cell line (T-47D). (1983)

The purpose of this study was to examine the lactogenic response of human mammary cancer cell lines to hormones in vitro. Progesterone was found to stimulate the incorporation of 14C from [14C]acetate into triglycerides (TG) and to promote accumulation of TG with a fatty acid composition similar to that of human milk fat in T-47D cells. Lipid droplets were observed in larger numbers without concomitant accumulation of casein granules in cells incubated with progesterone, but secretion of lipid into the medium did not occur. An effect of progesterone on TG accumulation was detectable after 12 hr and was maximal at 72 hr. Increasing doses of progesterone (10(-9) to 10(-5) M) caused a progressive increase in TG accumulation. The presence of cortisol and/or prolactin did not alter TG formation nor the dose response of the cells to progesterone. The growth rate of T-47D cells was not altered by the presence of progesterone in the medium. Neither of the human mammary cancer cell lines, MCF-7 and HBL-100, nor the human fibroblast cell lines, 28 and 857, responded to progesterone. The data indicate that, while the normally lactogenic hormones do not stimulate milk product biosynthesis in the cell lines tested, progesterone specifically stimulated synthesis and accumulation of TG in the T-47D cells.
Pulmonary Endothelial Cell Interactions with the Extracellular Matrix (2009)

This chapter contains sections titled: Components of Cell–ECM Interactions Functional Effect of EC–ECM Interactions in the Pulmonary Vasculature Conclusions and Perspectives References
Identification, Expression, and Characterization of a cDNA Encoding Human Endoplasmic Reticulum Mannosidase I, the Enzyme That Catalyzes the First Mannose Trimming Step in Mammalian Asn-linked Oligosaccharide Biosynthesis (1999)

We have isolated a full-length cDNA clone encoding a human alpha1, 2-mannosidase that catalyzes the first mannose trimming step in the processing of mammalian Asn-linked oligosaccharides. This enzyme has been proposed to regulate the timing of quality control glycoprotein degradation in the endoplasmic reticulum (ER) of eukaryotic cells. Human expressed sequence tag clones were identified by sequence similarity to mammalian and yeast oligosaccharide-processing mannosidases, and the full-length coding region of the putative mannosidase homolog was isolated by a combination of 5'-rapid amplification of cDNA ends and direct polymerase chain reaction from human placental cDNA. The open reading frame predicted a 663-amino acid type II transmembrane polypeptide with a short cytoplasmic tail (47 amino acids), a single transmembrane domain (22 amino acids), and a large COOH-terminal catalytic domain (594 amino acids). Northern blots detected a transcript of approximately 2.8 kilobase pairs that was ubiquitously expressed in human tissues. Expression of an epitope-tagged full-length form of the human mannosidase homolog in normal rat kidney cells resulted in an ER pattern of localization. When a recombinant protein, consisting of protein A fused to the COOH-terminal luminal domain of the human mannosidase homolog, was expressed in COS cells, the fusion protein was found to cleave only a single alpha1,2-mannose residue from Man(9)GlcNAc(2) to produce a unique Man(8)GlcNAc(2) isomer (Man8B). The mannose cleavage reaction required divalent cations as indicated by inhibition with EDTA or EGTA and reversal of the inhibition by the addition of Ca(2+). The enzyme was also sensitive to inhibition by deoxymannojirimycin and kifunensine, but not swainsonine. The results on the localization, substrate specificity, and inhibitor profiles indicate that the cDNA reported here encodes an enzyme previously designated ER mannosidase I. Enzyme reactions using a combination of human ER mannosidase I and recombinant Golgi mannosidase IA indicated that that these two enzymes are complementary in their cleavage of Man(9)GlcNAc(2) oligosaccharides to Man(5)GlcNAc(2).
Development of Human Cytomegalovirus–Specific T Cell Immunity during Primary Infection of Pregnant Women and Its Correlation with Virus Transmission to the Fetus (2007)

The development of adaptive T cell immunity after primary HCMV infection appears to be a complex and slow process until a memory T cell response develops. The T cell immune response appears to influence vertical HCMV transmission.
Metformin in cancer prevention and therapy. (2014)

The prevalence of diabetes is dramatically increasing worldwide. The results of numerous epidemiological studies indicate that diabetic population is not only at increased risk of cardiovascular complications, but also at substantially higher risk of many forms of malignancies. The use of metformin, the most commonly prescribed drug for type 2 diabetes, was repeatedly associated with the decreased risk of the occurrence of various types of cancers, especially of pancreas and colon and hepatocellular carcinoma. This observation was also confirmed by the results of numerous meta-analyses. There are however, several unanswered questions regarding the exact mechanism of the anticancer effect of metformin as well as its activity against various types of cancer both in diabetic and nondiabetic populations. In the present work we discuss the proposed mechanism(s) of anticancer effect of metformin and preclinical and clinical data suggesting its anticancer effect in different populations.