gebhart/patrepeval huggingface dataset:
Enabling Practical Backscatter Communication for On-body Sensors (2016)

In this paper, we look at making backscatter practical for ultra-low power on-body sensors by leveraging radios on existing smartphones and wearables (e.g. WiFi and Bluetooth). The difficulty lies in the fact that in order to extract the weak backscattered signal, the system needs to deal with self-interference from the wireless carrier (WiFi or Bluetooth) without relying on built-in capability to cancel or reject the carrier interference.
Vibrational properties of nanoscale materials: From nanoparticles to nanocrystalline materials (2003)

The vibrational density of states (VDOS) of nanoclusters and nanocrystalline materials is derived from molecular-dynamics simulations using empirical tight-binding potentials. The results show that the VDOS inside nanoclusters can be understood as that of the corresponding bulk system compressed by the capillary pressure. At the surface of the nanoparticles the VDOS exhibits a strong enhancement at low energies and shows structures similar to that found near flat crystalline surfaces. For the nanocrystalline materials an increased VDOS is found at high and low phonon energies, in agreement with experimental findings. The individual VDOS contributions from the grain centers, grain boundaries, and internal surfaces show that, in the nanocrystalline materials, the VDOS enhancements are mainly caused by the grain-boundary contributions and that surface atoms play only a minor role. Although capillary pressures are also present inside the grains of nanocrystalline materials, their effect on the VDOS is different than in the cluster case which is probably due to the intergrain coupling of the modes via the grain boundaries.
Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs (2013)

We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.
Barbiturates and adenine derivatives. Molecular structure of a hydrogen-bonded complex (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBarbiturates and adenine derivatives. Molecular structure of a hydrogen-bonded complexDonald Voet and Alexander RichCite this: J. Am. Chem. Soc. 1972, 94, 16, 5888–5891Publication Date (Print):August 1, 1972Publication History Published online1 May 2002Published inissue 1 August 1972https://pubs.acs.org/doi/10.1021/ja00771a057https://doi.org/10.1021/ja00771a057research-articleACS PublicationsRequest reuse permissionsArticle Views147Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Origin of germ cells and formation of new primary follicles in adult human ovaries (2004)

Recent reports indicate that functional mouse oocytes and sperm can be derived in vitro from somatic cell lines. We hypothesize that in adult human ovaries, mesenchymal cells in the tunica albuginea (TA) are bipotent progenitors with a commitment for both primitive granulosa and germ cells. We investigated ovaries of twelve adult women (mean age 32.8 +/- 4.1 SD, range 27-38 years) by single, double, and triple color immunohistochemistry. We show that cytokeratin (CK)+ mesenchymal cells in ovarian TA differentiate into surface epithelium (SE) cells by a mesenchymal-epithelial transition. Segments of SE directly associated with ovarian cortex are overgrown by TA, forming solid epithelial cords, which fragment into small (20 micron) epithelial nests descending into the lower ovarian cortex, before assembling with zona pellucida (ZP)+ oocytes. Germ cells can originate from SE cells which cover the TA. Small (10 micron) germ-like cells showing PS1 meiotically expressed oocyte carbohydrate protein are derived from SE cells via asymmetric division. They show nuclear MAPK immunoexpression, subsequently divide symmetrically, and enter adjacent cortical vessels. During vascular transport, the putative germ cells increase to oocyte size, and are picked-up by epithelial nests associated with the vessels. During follicle formation, extensions of granulosa cells enter the oocyte cytoplasm, forming a single paranuclear CK+ Balbiani body supplying all the mitochondria of the oocyte. In the ovarian medulla, occasional vessels show an accumulation of ZP+ oocytes (25-30 microns) or their remnants, suggesting that some oocytes degenerate. In contrast to males, adult human female gonads do not preserve germline type stem cells. This study expands our previous observations on the formation of germ cells in adult human ovaries. Differentiation of primitive granulosa and germ cells from the bipotent mesenchymal cell precursors of TA in adult human ovaries represents a most sophisticated adaptive mechanism created during the evolution of female reproduction. Our data indicate that the pool of primary follicles in adult human ovaries does not represent a static but a dynamic population of differentiating and regressing structures. An essential mission of such follicular turnover might be elimination of spontaneous or environmentally induced genetic alterations of oocytes in resting primary follicles.
Nanobeam photonic crystal cavity quantum dot laser (2010)

The lasing behavior of one dimensional GaAs nanobeam cavities with embedded InAs quantum dots is studied at room temperature. Lasing is observed throughout the quantum dot PL spectrum, and the wavelength dependence of the threshold is calculated. We study the cavity lasers under both 780 nm and 980 nm pump, finding thresholds as low as 0.3 microW and 19 microW for the two pump wavelengths, respectively. Finally, the nanobeam cavity laser wavelengths are tuned by up to 7 nm by employing a fiber taper in near proximity to the cavities. The fiber taper is used both to efficiently pump the cavity and collect the cavity emission.
Detection of erythromycin-resistant determinants by PCR (1996)

Erythromycin resistance determinants include Erm methylases, efflux pumps, and inactivating enzymes. To distinguish the different mechanisms of resistance in clinical isolates, PCR primers were designed so that amplification of the partial gene products could be detected in multiplex PCRs. This methodology enables the direct sequencing of amplified PCR products that can be used to compare resistance determinants in clinical strains. Further, this methodology could be useful in surveillance studies of erythromycin-resistant determinants.
Cutting Edge: Engagement of CD160 by its HLA-C Physiological Ligand Triggers a Unique Cytokine Profile Secretion in the Cytotoxic Peripheral Blood NK Cell Subset (2004)

CD160 is an Ig-like activating NK cell receptor expressed on the majority of circulating NK cells. This population corresponds to the nonproliferating, highly cytolytic, CD56dimCD16+ subset. CD160 engagement by HLA-C molecules mediates cytotoxic function. In this study, we report that upon specific activation by the physiological ligand HLA-C, or Ab cross-linking, CD160+ peripheral blood NK cells produce IFN-gamma, TNF-alpha, and IL-6. This unique CD160-mediated cytokine production differs from the one observed after CD16 engagement whose expression is also restricted to the CD56dim cytotoxic NK cell subset. As already reported for the CD160-mediated cytotoxic effector function, CD160-mediated cytokine production by peripheral blood-NK cells is negatively controlled by the killer Ig-like receptor CD158b. Thus, the CD160 receptor represents a unique triggering surface molecule expressed by cytotoxic NK cells that participates in the inflammatory response and determines the type of subsequent specific immunity.
Analysis of T cell receptor variability in tumor-infiltrating lymphocytes from a human regressive melanoma. Evidence for in situ T cell clonal expansion. (1993)

Malignant melanomas are often infiltrated by T lymphocytes. It is postulated that the presence of tumor-infiltrating lymphocytes (TIL) reflects ongoing immune responses against transformed cells. Such "responses" appear generally inefficient with the potential exception of infrequent clinical situations characterized by spontaneous tumor regression. We have characterized here the molecular structure of the T cell receptor beta chain expressed by TILs in a case of regressive melanoma. Advantage was taken of the PCR technology to study T lymphocytes directly without cell culture. Experimentally validated V beta subfamily specific primers were used to evaluate the V beta usage in TILs and control samples. Our results reveal that clonal T cell populations, precisely defined by their V-D-J junctional sequences, are amplified at the tumor site. The existence of such local antigen-driven selections support the hypothesis that antitumor responses may indeed take place in regressive melanoma.
Demultiplexer with 120 channels and 0.29-nm channel spacing (1998)

A 120-channel WDM demultiplexer was fabricated by etching a concave grating into a SiO/sub 2/-SiON-SiO/sub 2/-Si slab waveguide. The grooves of the grating reflected light by two instances of total internal reflection. The device was suitable for demultiplexing wavelength channels in the 1530-1565-nm range with a 0.29-nm (36 GHz) channel spacing. Each channel had a spectral width (full width at half maximum) of about 0.18 nm. The fiber-to-fiber insertion loss was between -20 dB and -30 dB for most channels. The loss was predominantly due to waveguide absorption and increased dramatically for wavelengths below 1539 nm.
From the Treaty to the present (2008)

Series preface Introduction, Vol III Part I Partition, the Treaty and Civil war: The Irish Free State passport and the question of citizenship, 1921-4, Joseph P. O'Grady The Irish civil war, 1922-1923, Brian P. Murphy The Catholic Church and partition, 1918-22, O.P. Rafferty. Part II Establishing Irish Free State, 1922-1932: The politics of reaction: the dynamics of Treatyite government and policy, 1922-33, John M. Regan The Irish Free State and the League of Nations, 1922-32, Michael Kennedy The construction and destruction of a colonial landscape: monuments to British monarchs in Dublin before and after independence, Y. Whelan. Part III The Age of Eamon De Valera, 1932-1948: Final exit? Britain, Eire, the Commonwealth and the repeal of the External Relations Act, 1945-1949, D.W. Dean 'Putting new wine into old bottles': the Irish Right and the embrace of European social thinking in the early 1930s, Mike Cronin 'Burn everything British but their coal': the Anglo-Irish economic war of the 1930s, Kevin H. O'Rourke Dublin slums in the 1930s, Frank Murphy The Blueshirts and the 'economic war': a study of Ireland in the context of dependency theory, Andrew W. Orridge Pariah dogs: deserters from the Irish defence forces who joined the British armed forces during 'the emergency', Liam Canny Catholic action and the development of the Irish welfare state in the 1930s and 1940s, Adrian Kelly. Part IV Northern Ireland, 1920-1968: 'Protestantism before Party!': the Ulster Protestant League in the 1930s, Graham Walker Creating jobs, manufacturing unity: Ulster Unionism and mass unemployment, 1922-34, Christopher Norton Lord Londonderry and education reform in 1920s Northern Ireland, Neil C. Fleming Northern Ireland and British Fascism in the inter-war years, James Loughlin Cultivating their own garden: broadcasting and culture in Northern ireland in the 1930s and 1940s, Gillian McIntosh Belfast republicanism in the 30s: the oral evidence, Bill Rolston and Ronnie Munck. Part V Ireland Since 1949: Ireland and the Marshall plan, Bernadette Whelan Gender and voter appeal in Irish elections, 1948-1997, Michael O'Kelly Changes in population and the extent of the built up area of the Dublin City region, 1936-1988, Arnold Horner. Part VI Northern Ireland Since 1968: Acts of union: youth, sub-culture and ethnic identity amongst Protestants in Northern Ireland, Desmond Bell Northern Irish nationalist political culture, Jennifer Todd 'This is not a rebel song': the Irish conflict and popular music, Bill Rolston From Sunningdale to the Good Friday Agreement: creating devolved government in Northern Ireland, Jonathan Tonge. Part VII Women: Surveying politics of peace, gender, conflict, and identity in Northern Ireland: the case of the Derry Peace Women in 1972, Marie Hammond Callaghan The hidden history of the PFIs: the repatriation of unmarried mothers and their children from England to Ireland in the 1950s and 1960s, Paul Michael Garrett Women and the Irish Free State, 1922-1939: the interaction between economics and ideology, Mary E. Daly Index.
Asymmetric Transfer Hydrogenation of Aromatic Ketones Catalyzed by Chiral Ruthenium(II) Complexes (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric Transfer Hydrogenation of Aromatic Ketones Catalyzed by Chiral Ruthenium(II) ComplexesShohei Hashiguchi, Akio Fujii, Jun Takehara, Takao Ikariya, and Ryoji NoyoriCite this: J. Am. Chem. Soc. 1995, 117, 28, 7562–7563Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/ja00133a037https://doi.org/10.1021/ja00133a037research-articleACS PublicationsRequest reuse permissionsArticle Views12269Altmetric-Citations1113LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Assignments and hydrogen bond sensitivities of UV resonance Raman bands of the C8‐deuterated guanine ring (2002)

Abstract Isotope‐edited Raman spectroscopy, a combination of site‐selective isotopic labeling and Raman difference spectroscopy, is a useful method for studying the structure and interaction of individual nucleic acid residues in oligonucleotides. To obtain basic data for applying isotope‐edited Raman spectroscopy to guanine residues, we studied the vibrational modes of UV resonance Raman bands of the C8‐deuterated guanine ring by examining the wavenumber shifts upon seven isotopic substitutions (2‐ 13 C, 2‐ 15 N, 6‐ 18 O, 7‐ 15 N, 8‐ 13 C, 9‐ 15 N and 1′‐ 13 C). The hydrogen bond sensitivities of the Raman bands were also investigated by comparing the Raman spectra recorded in several solvents of different hydrogen bonding properties. Some of the Raman bands were found to be markers of hydrogen bonding at specific donor or acceptor sites on the guanine ring. The Raman bands, which shift on C8‐deuteration, remain in the difference spectrum between the unlabeled and C8‐deuterated guanine rings. Among them, a negative peak around 1525 cm −1 and a strong positive/negative peak pair around 1485/1465 cm −1 serve as markers of hydrogen bonding at N7 and C6O, respectively. Another weak positive/negative peak pair around 1025/1040 cm −1 is sensitive to hydrogen bonding at the proton donor sites (N1—H and N2—H 2 ). The applicability of the hydrogen bond markers has been tested by using a 22‐mer oligonucleotide duplex containing eight guanine residues and its analog in which a single guanine residue is C8‐deuterated. The difference spectrum shows that the hydrogen bonding state of the guanine residue at the labeled position is consistent with the Watson–Crick base pair structure of DNA. Isotope‐edited Raman spectroscopy is a useful tool for studying the hydrogen bonding state of selected guanine residues in oligonucleotides. Copyright © 2002 John Wiley &amp; Sons, Ltd.
Characterization of Recombinant Human Coagulation Factor XFriuli (1996)

Summary Naturally occurring plasma factor XFriuli (pFXFr) is marginally activated by both the extrinsic and intrinsic coagulation pathways and has impaired catalytic potential. These studies were initiated to obtain confirmation that this molecule is multi-functionally defective due to the substitution of Ser for Pro at position 343 in the catalytic domain. By the Nelson-Long site-directed mutagenesis procedure a construct of cDNA in pRc/CMV was derived for recombinant factor XFriuli (rFXFr) produced in human embryonic (293) kidney cells. The rFXFr was purified and shown to have a molecular size identical to that of normal plasma factor X (pFX) by gel electrophoretic, and amino-terminal sequencing revealed normal processing cleavages. Using recombinant normal plasma factor X (rFXN) as a reference, the post-translational y-carboxy-glutamic acid (Gla) and (β-hydroxy aspartic acid (β-OH-Asp) content of rFXFr was over 85% and close to 100%, respectively, of expected levels. The specific activities of rFXFr in activation and catalytic assays were the same as those of pFXFr. Molecular modeling suggested the involvement of a new H-bond between the side-chains of Ser-343 and Thr-318 as they occur in anti-parallel (3-pleated sheets near the substrate-binding pocket of pFXFr. These results support the conclusion that the observed mutation in pFXFr is responsible for its dysfunctional activation and catalytic potentials, and that it accounts for the moderate bleeding tendency in the homozygous individuals who possess this variant procoagulant.
5-HT1A-Receptor Subtype Mediates the Effect of Fluvoxamine, a Selective Serotonin Reuptake Inhibitor, on Marble-Burying Behavior in Mice (1995)

The effect of fluvoxamine, a selective serotonin (5-HT) reuptake inhibitor, was studied in a model of anxiety and/or obsessive compulsive disorder (OCD) in mice. In the anxiety/OCD model, marble-burying behavior, marble-burying was significantly suppressed by fluvoxamine at 30 and 60 mg/kg, p.o. and the monoamine reuptake inhibitor clomipramine, at 60 mg/kg, p.o. No suppressive effect, however, was observed by the selective norepinephrine reuptake inhibitor desipramine at doses from 15 to 60 mg/kg, p.o. Suppressive effects were obtained by the serotonergic anxiolytic buspirone at 30 and 60 mg/kg, p.o. and the benzodiazepine anxiolytic diazepam at 10 mg/kg, p.o. The effect of fluvoxamine on marble-burying was slightly attenuated after repeated administration. On the other hand, both the effects of buspirone and diazepam completely disappeared after repeated administration. Effect of fluvoxamine on the marble-burying was unaffected by the 5-HT2 antagonist ritanserin. However, the 5-HT1A antagonist NAN-190 (1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl] piperazine) inhibited the suppressive effect of fluvoxamine on the marble-burying. From these results, the 5-HT1A-receptor subtype may be involved in the suppressive effect of fluvoxamine on the marble-burying, but the 5-HT2-receptor subtype is not involved in this effect.
Plasticity of epidermal adult stem cells derived from adult goat ear skin (2006)

Here we report the isolation and characterization of pluripotent stem cells from adult goat skin. We found that these primary cells have the properties of embryonic stem cells (ESC), including the expression of appropriate immunological markers and the capability of forming embryoid bodies. The subcultured cells also show the characteristics of stem cells, such as the expression of CK19, beta(1-)integrin, P63, and formation of holo-clones in culture. Therefore, we termed these cells epidermal adult stem cells (EpiASC), although their origin was not identified. We have shown that clones of individual EpiASC proliferate and differentiate in culture to produce neurons, cardiomyocytes, osteoblasts, and occytes. Further, we cultivated EpiASC on bioengineered dermis and denuded human amniotic membrane (HAM), to reconstruct artificial skin and corneal epithelium. We successfully transplanted those artificial tissues in goats with acute full-thickness skin defect (AFTSD) and limbal stem cell deficiency (LSCD), respectively. Our results showed that indeed EpiASC reconstructed the skin (hair was observed in restored areas), and repaired the damaged cornea of goats with total LSCD. These data confirm that EpiASC can differentiate into different functional cell types in vivo or in vitro. Due to their high degree of inherent plasticity, and to their easy accessibility for collection from the skin, EpiASC are excellent candidate sources for diverse cell therapies.
Novel insights into the clinical manifestations and treatment of hypertrophic cardiomyopathy (1995)

Knowledge of the pathophysiology, clinical manifestations, and treatment of hypertrophic cardiomyopathy continues to evolve. In subaortic obstructive hypertrophic cardiomyopathy, the importance of elongated mitral leaflets in causing the outflow obstruction and concomitant mitral regurgitation has been further elucidated, and marked worsening of the obstruction during upright exercise has been clearly demonstrated. Treatment options now include dual chamber pacing as well as medical and surgical therapy. The syndrome of midventricular obstruction with apical infarction and aneurysm formation most commonly results from apical infarction in the presence of extensive apical hypertrophy, with the noninfarcted midventricular hypertrophic tissue responsible for the midventricular obstruction. Attention is drawn to the variable pathophysiology and frequency of subaortic obstruction in hypertrophic cardiomyopathy in the elderly, the etiology of which is probably multifactorial. Finally, the interrelationship of impaired ventricular relaxation, coronary flow dynamics, and myocardial ischemia is discussed.