gebhart/patrepeval huggingface dataset:
Heterologous protein production using the Pichia pastoris expression system (2005)

The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins. Recent developments with respect to the Pichia expression system have had an impact on not only the expression levels that can be achieved, but also the bioactivity of various heterologous proteins. We review here some of these recent developments, as well as strategies for reducing proteolytic degradation of the expressed recombinant protein at cultivation, cellular and protein levels. The problems associated with post-translational modifications performed on recombinant proteins by P. pastoris are discussed, including the effects on bioactivity and function of these proteins, and some engineering strategies for minimizing unwanted glycosylations. We pay particular attention to the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed. Finally, future aspects of the use of the P. pastoris expression system are discussed with regard to the production of complex membrane proteins, such as G protein-coupled receptors, and the industrial and clinical importance of these proteins.
Substituted oxindoles. Part VI. Polarographic reduction of substituted trans-3-benzylideneindol-2(3H)-ones (1973)

The polarographic reduction of a number of substituted 3-benzylideneindol-2(3H)-ones in ethanol–water (3:2 v/v) has been studied. These exhibit one or two reduction waves depending on the supporting electrolyte and the pH of the solution. Changes in E½ are discussed in terms of the Hammett σ values.
No-reference quality assessment using natural scene statistics: JPEG2000 (2005)

Measurement of image or video quality is crucial for many image-processing algorithms, such as acquisition, compression, restoration, enhancement, and reproduction. Traditionally, image quality assessment (QA) algorithms interpret image quality as similarity with a "reference" or "perfect" image. The obvious limitation of this approach is that the reference image or video may not be available to the QA algorithm. The field of blind, or no-reference, QA, in which image quality is predicted without the reference image or video, has been largely unexplored, with algorithms focusing mostly on measuring the blocking artifacts. Emerging image and video compression technologies can avoid the dreaded blocking artifact by using various mechanisms, but they introduce other types of distortions, specifically blurring and ringing. In this paper, we propose to use natural scene statistics (NSS) to blindly measure the quality of images compressed by JPEG2000 (or any other wavelet based) image coder. We claim that natural scenes contain nonlinear dependencies that are disturbed by the compression process, and that this disturbance can be quantified and related to human perceptions of quality. We train and test our algorithm with data from human subjects, and show that reasonably comprehensive NSS models can help us in making blind, but accurate, predictions of quality. Our algorithm performs close to the limit imposed on useful prediction by the variability between human subjects.
Nonlinear approximation based image recovery using adaptive sparse reconstructions and iterated denoising-part I: theory (2006)

We study the robust estimation of missing regions in images and video using adaptive, sparse reconstructions. Our primary application is on missing regions of pixels containing textures, edges, and other image features that are not readily handled by prevalent estimation and recovery algorithms. We assume that we are given a linear transform that is expected to provide sparse decompositions over missing regions such that a portion of the transform coefficients over missing regions are zero or close to zero. We adaptively determine these small magnitude coefficients through thresholding, establish sparsity constraints, and estimate missing regions in images using information surrounding these regions. Unlike prevalent algorithms, our approach does not necessitate any complex preconditioning, segmentation, or edge detection steps, and it can be written as a sequence of denoising operations. We show that the region types we can effectively estimate in a mean-squared error sense are those for which the given transform provides a close approximation using sparse nonlinear approximants. We show the nature of the constructed estimators and how these estimators relate to the utilized transform and its sparsity over regions of interest. The developed estimation framework is general, and can readily be applied to other nonstationary signals with a suitable choice of linear transforms. Part I discusses fundamental issues, and Part II is devoted to adaptive algorithms with extensive simulation examples that demonstrate the power of the proposed techniques.
Insulin-Like Growth Factor I: a Modulator of Erythropoiesis in Uraemic Patients? (1992)

Anaemia is a feature almost invariably complicating chronic renal failure. Its pathophysiology is multifactorial but the most important cause is erythropoietin (Epo) deficiency. However, either no relation or even a weakly positive relation generally exists between serum immunoreactive (i) Epo and haematocrit values in uraemic anaemia, whereas in anaemias of non-renal origin the correlation is most often strongly negative. Recent evidence indicates that growth hormone also stimulates erythropoiesis. Moreover, late erythroid progenitor cells (CFU-E) require insulin and/or insulin-like growth factor I (IGF-I) for development in vitro. IGF-I has been shown to have a synergistic action with Epo. We have measured serum iEpo and IGF-I levels in 17 haemodialysis patients with severe hyperparathyroidism (mean +/- SEM serum iPTH, 988 +/- 88 pg/ml). Mean age and duration of dialysis treatment were 46.1 +/- 3.4 and 8.8 +/- 1.0 years respectively. Mean haematocrit and haemoglobin values wer 28.1 +/- 1.7% and 9.39 +/- 0.54 g/dl respectively. Mean serum iEpo and IGF-I levels were 20.3 +/- 4.7 mU/ml and 320 +/- 20 ng/ml respectively (normal values for serum iEpo and IGF-I, 17.9 +/- 6 mU/ml and 91 +/- 23 ng/ml respectively). We found that serum IGF-I concentrations were well correlated with haematocrit values (r = 0.68, n = 15, P less than 0.004) whereas serum iEpo values were not (r = 0.41, n = 12, P = 0.18). IGF-I could therefore be an important factor regulating erythropoiesis in uraemic patients, at least when associated with severe hyperparathyroidism.
Chapitre II. Neutralité et propagandes (1990)

De septembre 1939 aux déroutes de l’année 1940, la situation de guerre en Europe implique une prise de position du gouvernement mexicain de Lázaro Cárdenas ; mais elle ne peut se définir du strict point de vue mexicain et doit nécessairement prendre en considération celle de Washington. La position mexicaine s’individualise toutefois sur le continent américain par le soutien effectif et continu aux vaincus espagnols... dont nombre sont réfugiés en territoire français. La diplomatie française ...
원저 : 성인에서 비만에 대한 자가 평가 (2002)

연구배경: 일반인들의 비만에 대한 자가평가는 객관적인 기준에 의하기보다는 주관적인 것으로, 이는 체중조절에 대한 부적절한 노력을 이끌 가능성이 있다. 본 연구는 우리나라 성인을 대상으로 비만에 대한 자가평가와 체질량지수를 기준으로 정의한 객관적인 비만도 사이의 차이를 알아보고, 비만에 대한 인식에 영향을 주는 사회환경적 요인을 규명해 보고자 하였다. 방법: 가천의대 길병원의 건강증진 센터에 내원하여, 건강검진을 받은 561명(남 341: 여 220)의
Three-dimensional structure of a cysteine-rich repeat from the low-density lipoprotein receptor. (1995)

The low-density lipoprotein (LDL) receptor plays a central role in mammalian cholesterol metabolism, clearing lipoproteins which bear apolipoproteins E and B-100 from plasma. Mutations in this molecule are associated with familial hypercholesterolemia, a condition which leads to an elevated plasma cholesterol concentration and accelerated atherosclerosis. The N-terminal segment of the LDL receptor contains a heptad of cysteine-rich repeats that bind the lipoproteins. Similar repeats are present in related receptors, including the very low-density lipoprotein receptor and the LDL receptor-related protein/alpha 2-macroglobulin receptor, and in proteins which are functionally unrelated, such as the C9 component of complement. The first repeat of the human LDL receptor has been expressed in Escherichia coli as a glutathione S-transferase fusion protein, and the cleaved and purified receptor module has been shown to fold to a single, fully oxidized form that is recognized by the monoclonal antibody IgG-C7 in the presence of calcium ions. The three-dimensional structure of this module has been determined by two-dimensional NMR spectroscopy and shown to consist of a beta-hairpin structure, followed by a series of beta turns. Many of the side chains of the acidic residues, including the highly conserved Ser-Asp-Glu triad, are clustered on one face of the module. To our knowledge, this structure has not previously been described in any other protein and may represent a structural paradigm both for the other modules in the LDL receptor and for the homologous domains of several other proteins. Calcium ions had only minor effects on the CD spectrum and no effect on the 1H NMR spectrum of the repeat, suggesting that they induce no significant conformational change.
Entry of Listeria monocytogenes into hepatocytes requires expression of InIB, a surface protein of the internalin multigene family (1995)

The intracellular bacterium Listeria monocytogenes can invade several types of normally non-phagocytic cells. Entry into cultured epithelial cells requires the expression of inIA, the first gene of an operon, comprising two genes: inIA, which encodes internalin, an 800-amino-acid protein, and inIB, which encodes a 630-amino-acid protein. Several genes homologous to inIA are detected in the genome of L. monocytogenes; InIB is one of them. We have assessed the role of inIB in invasiveness of L. monocytogenes by constructing isogenic chromosomal deletion mutants in the inIAB locus. Our findings indicate that: i) inIB is required for entry of L. monocytogenes into hepatocytes, but not into intestinal epithelial cells; ii) inIB encodes a surface protein; iii) internalin plays a role for entry into some hepatocyte cell lines. These results provide the first insight into the cell tropism displayed by L. monocytogenes.
Full-thickness oesophageal regeneration in pig using a polyurethane mucosal cell seeded graft (2016)

Malignant oesophageal pathology typically requires resection of a portion of oesophagus. The aim of this study was to investigate attachment and growth of swine oesophageal mucosal cells on electrospun synthetic nanofibre matrices of varying chemistries and to determine whether a mucosal-seeded graft, in a swine animal model, could induce regeneration. Swine mucosal oesophageal cells were isolated and seeded them onto five different matrix materials. Matrix samples were cultured for up to 14 days, after which matrices were analysed for cell attachment. Attachment varied for each of the matrix materials tested, with the most rigid showing the lowest levels of attachment. Importantly, sections of these matrices illustrated that multiple layers of mucosal cells formed, mimicking endogenous oesophageal structure. A tdTomato reporter line (mucosal<sup>tdt</sup> cells) was created to enable cell tracking. As polyurethane matrix was found optimal through in vitro testing, a graft was prepared using mucosal<sup>tdt</sup> cells, along with an unseeded control, and implanted into swine for determination of oesophageal regeneration. Mucosal seeded polyurethane grafts initiated full thickness regeneration of the oesophagus, including epithelial, submucosal, and skeletal muscle layers which were highly vascularized. Interestingly, an unseeded graft showed similar regeneration, indicating that the role of cells in the process of oesophageal regeneration is still unclear. The electrospun polyurethane matrix does appear suitable for multilayered cellular attachment and growth of oesophageal mucosal cells, and implantation of polyurethane grafts initiated full thickness regeneration of the oesophagus, indicating potential for oesophageal reconstruction in humans. Copyright © 2016 John Wiley & Sons, Ltd.
Low driving voltage or current digital optical switch on InP for multiwavelength system applications (1992)

Low driving voltage or current digital optical switches based on carrier effects in InP/GaInAsP have been fully demonstrated for both TE and TM polarisations of light. Crosstalk better than 12.1 and 15.2 dB have been obtained for polarisation independent operation with, respectively, a voltage as low as – 10 V and a current as low as 6 mA. These functionalities have been demonstrated in the 1.52–1.57 μm wavelength range which is suitable for multiwavelength system applications.
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE (1958)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
The biochemistry of silicic acid (1938)

Research Article| February 01 1938 The biochemistry of silicic acid: The solution and excretion of silica Earl Judson King; Earl Judson King 1The British Postgraduate Medical School, London Search for other works by this author on: This Site PubMed Google Scholar Murray McGeorge Murray McGeorge 1The British Postgraduate Medical School, London Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1938) 32 (2): 426–433. https://doi.org/10.1042/bj0320426 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation Earl Judson King, Murray McGeorge; The biochemistry of silicic acid: The solution and excretion of silica. Biochem J 1 February 1938; 32 (2): 426–433. doi: https://doi.org/10.1042/bj0320426 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1938 CAMBRIDGE UNIVERSITY PRESS1938 Article PDF first page preview Close Modal You do not currently have access to this content.
The thylakoid translocation of subunit 3 of photosystem I, the psaF gene product, depends on a bipartite transit peptide and proceeds along an azide-sensitive pathway (1994)

Subunit 3 of photosystem I (PSI-3), the product of the nuclear psaF gene, is the docking protein for plastocyanin during photosynthetic electron transport in thylakoid membranes and is synthesized in the cytosol with a transit peptide that resembles structurally the bipartite targeting signals of hydrophilic, lumenal components such as plastocyanin. In organello import experiments performed with the authentic PSI-3 precursor and chimeric polypeptides consisting of residue-correct fusions of transit peptides and mature proteins derived from different plastid proteins demonstrate that the PSI-3 transit peptide is indeed capable of translocating proteins into the thylakoid lumen and that, conversely, mature PSI-3 depends on a bipartite transit peptide for its thylakoid transfer. Of the three recently described translocation/integration pathways for nucleus-encoded proteins carrying bipartite transit peptides that are distinct in their physiological requirements and strictly protein-specific, PSI-3, like plastocyanin and the 33-kDa protein of the oxygen-evolving complex, is translocated by a pathway that involves stromal factors but no proton gradient across the membrane. It is not affected by saturating amounts of the precursor for the 23-kDa protein of the oxygen-evolving complex that follows the latter route. Thylakoid translocation of PSI-3 is, however, impaired in the presence of sodium azide, which indicates that a homolog to the bacterial SecA protein might be involved in this process suggesting, thus, a prokaryote-like translocation pathway. The azide-sensitive factor appears to interact predominantly with the transit peptide of a precursor protein, since chimeras consisting of a presequence from an azide-resistant precursor and a mature part of an azide-sensitive polypeptide are still translocated in the presence of the inhibitor.
Specific detection of proteins using photonic crystal waveguides (2008)

Specific detection of proteins is demonstrated using planar photonic crystal waveguides. Using immobilized biotin as probe, streptavidin was captured, causing the waveguide mode cut-off to red-shift. The device was shown to detect a 2.5 nm streptavidin film with a 0.86 nm cut-off red-shift. An improved photonic crystal waveguide sensor design is also described and shown to have a 40% improved bulk refractive index response.
Introduction to Computational Chemistry (1998)

"Introduction to Computational Chemistry 3rd Edition provides a comprehensive account of the fundamental principles underlying different computational methods. Fully revised and updated throughout to reflect important method developments and improvements since publication of the previous edition, this timely update includes the following significant revisions and new topics: Polarizable force fields; Tight-binding DFT; More extensive DFT functionals, excited states and time dependent molecular properties; Accelerated Molecular Dynamics methods; Tensor decomposition methods; Cluster analysis; Reduced scaling and reduced prefactor methods" -- From the publisher.
Cloning, sequencing, and expression of a cDNA encoding rat LIMP II, a novel 74-kDa lysosomal membrane protein related to the surface adhesion protein CD36. (1991)

LIMP II is a glycoprotein expressed in the membrane of lysosomes and secretory granules with lysosomal properties. Sequence analysis of a CNBr-cleaved peptide allowed the synthesis of a 47-mer oligonucleotide that was used to screen a rat liver cDNA library in lambda gt11. This resulted in isolation of a 2-kilobase cDNA containing 1,434 bases encoding the entire protein. The deduced amino acid sequence indicates that LIMP II consists of 478 amino acid residues. The segment spanning residues 4-6 to 26 constitute an uncleavable signal peptide. LIMP II possesses a hydrophobic amino acid segment near the carboxyl end, that together with the uncleaved signal peptide may anchor the protein to the membrane through two distant segments. The major portion of the protein resides on the luminal side and displays 11 potential N-glycosylation sites and 5 cysteine residues. Two short cytoplasmic tails, 2-4 and 20-21 amino acids long, correspond to the NH2- and COOH-terminal ends of the protein, respectively. Transfection of COS cells with the cDNA of LIMP II resulted in expression of the protein and its transport to lysosomes. Comparison of the entire sequence to various data bases of known proteins revealed extensive homology between LIMP II and the cell surface protein CD36 involved in cell adhesion. No significant homology was detected with the two families of lysosomal membrane proteins A and B, recently described.
On Channel Selection Strategies for Multi-Channel MAC Protocols in Wireless Ad Hoc Networks (2006)

Multi-channel medium access control (MAC) protocols have recently been proposed to improve the performance of the transmission control protocol (TCP) in IEEE 802.11 wireless ad hoc networks. This paper uses ns-2 network simulations to study the impact of channel selection techniques on multi-channel MAC protocol performance, particularly for the bi-directional multi-channel MAC protocol. Three channel selection strategies are studied: random, lowest channel first, and soft channel reservation. The simulation results identify four distinct scenarios in which data channel frame losses can occur. Among the channel selection strategies evaluated, the soft channel reservation technique is the most effective for the missed reservation problem. This channel selection strategy reduces link-layer data frame losses and provides higher TCP throughput compared to the other channel selection approaches
Efficient Solid-Phase Synthesis of Peptide-Substituted Alkanethiols for the Preparation of Substrates That Support the Adhesion of Cells (1998)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTEfficient Solid-Phase Synthesis of Peptide-Substituted Alkanethiols for the Preparation of Substrates That Support the Adhesion of CellsBenjamin T. Houseman and Milan MrksichView Author Information Department of Chemistry, The University of Chicago, Chicago, Illinois 60637 Cite this: J. Org. Chem. 1998, 63, 21, 7552–7555Publication Date (Web):September 22, 1998Publication History Received10 June 1998Published online22 September 1998Published inissue 1 October 1998https://pubs.acs.org/doi/10.1021/jo981113shttps://doi.org/10.1021/jo981113sbrief-reportACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views807Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Alcohols,Cell physiology,Monolayers,Organic polymers,Peptides and proteins Get e-Alerts
Structure solution by iterative peaklist optimization and tangent expansion in space group P1 (1995)

An extension to the peaklist optimization procedure is proposed, in which one overall phase refinement cycle consists of tangent expansion, E-map, peaksearch and elimination of peaks to achieve a maximum correlation coefficient between E o and E c . This procedure appears to be able to solve large structures from random phases given data to atomic resolution. The power of the method can be substantially increased by starting with slightly better than random phases, obtained for example from threefold Patterson vector superposition minimum functions or rotation searches using a fragment of known geometry. These two sources of phase information require expansion of the data to the space group P1, which also appears to be a useful strategy when starting from random phases. This real/reciprocal space recycling procedure was successful in solving two small known proteins and three unknown 200+-atom small-molecule structures. An investigation of the influence of the resolution on the peaklist optimization algorithm shows that there is a marked deterioration in the effectiveness as the resolution of the data is truncated, the deterioration being particularly marked between 1.2 and 1.3 A