gebhart/patrepeval huggingface dataset:
Flexible and Optimal Design of Spherical Microphone Arrays for Beamforming (2007)

This paper describes a methodology for designing a flexible and optimal spherical microphone array for beamforming. Using the approach presented, a spherical microphone array can have very flexible layouts of microphones on the spherical surface, yet optimally approximate a desired beampattern of higher order within a specified robustness constraint. Depending on the specified beampattern order, our approach automatically achieves optimal performances in two cases: when the specified beampattern order is reachable within the robustness constraint we achieve a beamformer with optimal approximation of the desired beampattern; otherwise we achieve a beamformer with maximum directivity, both robustly. For efficient implementation, we also developed an adaptive algorithm for computing the beamformer weights. It converges to the optimal performance quickly while exactly satisfying the specified frequency response and robustness constraint in each step. One application of the method is to allow the building of a real-world system, where microphones may not be placeable on regions, such as near cable outlets and/or a mounting base, while having a minimal effect on the performance. Simulation results are presented
The end of the message: 3'– end processing leading to polyadenylated messenger RNA (1992)

Almost all messenger RNAs carry a polyadenylate tail that is added in a post-transcriptional reaction. In the nuclei of animal cells, the 3'-end of the RNA is formed by endonucleolytic cleavage of the primary transcript at the site of poly(A) addition, followed by the polymerisation of the tail. The reaction depends on specific RNA sequences upstream as well as downstream of the polyadenylation site. Cleavage and polyadenylation can be uncoupled in vitro. Polyadenylation is carried out by poly(A) polymerase with the aid of a specificity factor that binds the polyadenylation signal AAUAAA. Several additional factors are required for the initial cleavage. A newly discovered poly(A)-binding protein stimulates poly(A) tail synthesis and may be involved in the control of tail length. Polyadenylation reactions different from this scheme, either in other organisms or under special physiological circumstances, are discussed.
Monophosphoryl Lipid A Activates Both Human Dendritic Cells and T Cells (2002)

The induction of dendritic cell (DC) maturation is critical for the induction of Ag-specific T lymphocyte responses and may be essential for the development of human vaccines relying on T cell immunity. In this study, we have investigated the effects of monophosphoryl lipid A (MPL) on human monocyte-derived DC as well as peripheral blood T cells. Calcium mobilization, mitogen-activated protein kinase activation, and the NF-kappaB transcription factor were induced after MPL stimulation of DC and required high doses of MPL (100 microg/ml). Maturation parameters such as production of IL-12 and increases in cell surface expression of HLA-DR, CD80, CD86, CD40, and CD83 were observed following DC treatment with MPL. However, lower levels of IL-12 were induced by MPL when compared with lipopolysaccharide. This is likely to be related to differences in the kinetics of extracellular signal-related kinase 1/2 and p-38 phosphorylation induced by both molecules. Although maturation induced by MPL was weaker when compared with lipopolysaccharide, it appeared to be sufficient to support optimal activation of allogeneic naive CD45RA(+) T cell and anti-tetanus toxoid CD4 T cells. MPL at low doses (5 microg/ml) had no impact on DC maturation, while its addition to DC-T cell cocultures induced full T cell activation. The observed effect was related to the fact that MPL also acts directly on T cells, likely through their Toll-like receptors, by increasing their intracellular calcium and up-regulating their CD40 ligand expression. Together, these data support a model where MPL enhances T cell responses by having an impact on DC and T cells.
Microtubule-associated protein 2, a microtubule-associated protein from brain, is immunologically related to the alpha subunit of erythrocyte spectrin. (1982)

MAP, microtubule-associated protein; EGTA, ethylene glycol bis(P-aminoethyl ether)-N,N,N',
Melevodopa/carbidopa effervescent formulation in the treatment of motor fluctuations in advanced Parkinson's disease (2010)

Melevodopa hydrochloride plus carbidopa in effervescent tablets (M/C) is a readily soluble antiparkinsonian tablet formulation. A total of 221 patients with Parkinson's disease and motor fluctuations entered a randomized, double-blind, double-dummy, controlled parallel group study, which compared the effectiveness of oral M/C effervescent tablets with standard oral formulation levodopa/carbidopa tablets (L/C; Sinemet) in reducing total daily OFF time. The difference of total daily OFF time (intention-to-treat population) between the two groups was not statistically significant (P = 0.07): -39.4 minutes (95%CI: -67.08 to -11.73) in M/C group vs. +3.5 minutes (95%CI: -36.19 to +43.26) in the L/C group. In the intragroup analysis, M/C significantly reduced the baseline daily OFF, which remained unchanged in the L/C group. There were no unexpected adverse events in either treatment arms, and discontinuation rates due to adverse events did not differ between the two groups [M/C: 2 patients (1.3%); L/C: 1 patient (1.4%)]. This study failed to meet the primary endpoint (P = 0.07); however, there was a trend in favour of the M/C preparation, which deserves further attention.
Polar-direct-drive experiments on the National Ignition Facilitya) (2015)

To support direct-drive inertial confinement fusion experiments at the National Ignition Facility (NIF) [G. H. Miller, E. I. Moses, and C. R. Wuest, Opt. Eng. 43, 2841 (2004)] in its indirect-drive beam configuration, the polar-direct-drive (PDD) concept [S. Skupsky et al., Phys. Plasmas 11, 2763 (2004)] has been proposed. Ignition in PDD geometry requires direct-drive–specific beam smoothing, phase plates, and repointing the NIF beams toward the equator to ensure symmetric target irradiation. First experiments to study the energetics and preheat in PDD implosions at the NIF have been performed. These experiments utilize the NIF in its current configuration, including beam geometry, phase plates, and beam smoothing. Room-temperature, 2.2-mm-diam plastic shells filled with D2 gas were imploded with total drive energies ranging from ∼500 to 750 kJ with peak powers of 120 to 180 TW and peak on-target irradiances at the initial target radius from 8 × 1014 to 1.2 × 1015 W/cm2. Results from these initial experiments are presented, including measurements of shell trajectory, implosion symmetry, and the level of hot-electron preheat in plastic and Si ablators. Experiments are simulated with the 2-D hydrodynamics code DRACO including a full 3-D ray-trace to model oblique beams, and models for nonlocal electron transport and cross-beam energy transport (CBET). These simulations indicate that CBET affects the shell symmetry and leads to a loss of energy imparted onto the shell, consistent with the experimental data.
Nucleobase Modified Peptide Nucleic Acid (2003)

The Pd0/Cu1 catalyzed cross-coupling of terminal alkynes onto peptide nucleic acid monomers or submonomers bearing iodinated nucleobases has been utilized as a route to base-modified oligomers. Both 5-iodouracil and 5-iodocytosine derivatives undergo the cross-coupling to give the expected products in moderate to good yields. However, depending on the particular substrates and reaction conditions, the cross-coupling may be followed by a ring closing reaction to give the fluorescent furano- and pyrrolo-fused uracil and cytosine derivatives, respectively.
Cytolysis by tumor necrosis factor is preceded by a rapid and specific dissolution of microfilaments. (1989)

Tumor necrosis factor (TNF) is cytotoxic to certain transformed cells, whereas normal cells are resistant to its effects. The resistance of normal cells can often be overcome by treatment with inhibitors of transcription or translation such as actinomycin D or cycloheximide (CHI), suggesting that normal cells produce a protein(s) that protects them from TNF-induced cytolysis. In this report, we examine the mechanism of cytolysis in a 3T3-like mouse cell line, C3HA, which was sensitized to TNF by treatment with CHI. We found that an early change in TNF/CHI-treated cells was a significant loss of stress fibers in perinuclear areas of the cytoplasm. The disruption of microfilaments, which was observed within 15 min of treatment, was not seen in untreated cells or in cells treated with either TNF or CHI alone. The dissolution of microfilaments spread peripherally over time and preceded other TNF/CHI-induced effects such as cytoplasmic "boiling," decrease in cell volume, and lysis of the plasma membrane. The breakdown of stress fibers occurred without a change in microtubules or intermediate filaments. Cytochalasin E, which disrupts microfilaments, induced cytolysis of TNF-treated cells even in the absence of CHI; however, demecolcine, which depolymerizes microtubules, did not sensitize cells to TNF. We propose that the TNF-induced cytolysis of certain cell types is preceded by a selective disruption of the microfilament lattice.
Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T Cells (1998)

T helper cell type 1 (Th1) and 2 (Th2) are central to immune regulation. However, no stable cell surface marker capable of distinguishing and separating these two subsets of CD4(+) cells has yet been found. Using differential display PCR, we have identified a gene encoding a cell membrane bound molecule, originally designated ST2L, T1, DER4, or Fit, expressed constitutively and stably on the surface of murine Th2s, but not Th1s even after stimulation with a range of immunological stimuli. Antibody against a peptide derived from ST2L strongly and stably labeled the surface of cloned Th2s but not Th1s, and Th2s but not Th1s derived from naive T cells of ovalbumin T cell receptor-alpha/beta transgenic mice. Three-color single cell flow cytometric analysis shows that cell surface ST2L coexpressed with intracellular interleukin (IL)-4, but not with interferon (IFN)-gamma. The antibody selectively lysed Th2s in vitro in a complement-dependent manner. In vivo, it enhanced Th1 responses by increasing IFN-gamma production and decreasing IL-4 and IL-5 synthesis. It induced resistance to Leishmania major infection in BALB/c mice and exacerbated collagen-induced arthritis in DBA/1 mice. Thus, ST2L is a stable marker distinguishing Th2s from Th1s and is also associated with Th2 functions. Hence, it may be a target for therapeutic intervention.
PRSS8 methylation and its significance in esophageal squamous cell carcinoma (2016)

Esophageal cancer is one of the most common cancers worldwide, and the incidence and mortality is increasing rapidly in recent years in China, but the underlying mechanisms are largely unclear. Herein we found that the expression of PRSS8, a serine protease prostasin, is significantly decreased in esophageal squamous cell carcinomas (ESCC) at mRNA and protein levels. The reduction of PRSS8 was well correlated with poor differentiation and shorter survival time. Interestingly, ESCC stromal expression of PRSS8 was significantly correlated with stromal lymphocyte infiltration and cancer progression. Methylation specific PCR showed that PRSS8 was hypermethylated in ESCC tissues and ESCC cell lines, which was linked to the downregulation of PRSS8 expression and decreased activities of PRSS8 promoter. De-methylation agent decitabine was able to restore PRSS8 expression, leading to the inhibition of cancer cell proliferation, motility, migration and cell cycle arrest. However, the restored PRSS8 and its tumor inhibition could be reversed by small interfering RNA targeting PRSS8. Mechanistic study showed that tumor inhibition of PRSS8 may be associated with proliferation- and epithelial mesenchymal transition - related proteins in ESCC cells. In conclusion, our finding showed that PRSS8 methylation and its stromal expression had important clinical significance in ESCC.
Galleria mellonellainfection models for the study of bacterial diseases and for antimicrobial drug testing (2016)

Galleria mellonella (greater wax moth or honeycomb moth) has been introduced as an alternative model to study microbial infections. G. mellonella larvae can be easily and inexpensively obtained in large numbers and are simple to use as they don't require special lab equipment. There are no ethical constraints and their short life cycle makes them ideal for large-scale studies. Although insects lack an adaptive immune response, their innate immune response shows remarkable similarities with the immune response in vertebrates. This review gives a current update of what is known about the immune system of G. mellonella and provides an extensive overview of how G. mellonella is used to study the virulence of Gram-positive and Gram-negative bacteria. In addition, the use of G. mellonella to evaluate the efficacy of antimicrobial agents and experimental phage therapy are also discussed. The review concludes with a critical assessment of the current limitatons of G. mellonella infection models.
Sequence and secondary structure of moose 28S rRNA 5′terminal domain. Organisation of the 5.8S-28S rRNA complex (1982)

We present the sequence of the 5' terminal 585 nucleotides of mouse 28S rRNA as inferred from the DNA sequence of a cloned gene fragment. The comparison of mouse 28S rRNA sequence with its yeast homolog, the only known complete sequence of eukaryotic nucleus-encoded large rRNA (see ref. 1, 2) reveals the strong conservation of two large stretches which are interspersed with completely divergent sequences. These two blocks of homology span the two segments which have been recently proposed to participate directly in the 5.8S-large rRNA complex in yeast (see ref. 1) through base-pairing with both termini of 5.8S rRNA. The validity of the proposed structural model for 5.8S-28S rRNA complex in eukaryotes is strongly supported by comparative analysis of mouse and yeast sequences: despite a number of mutations in 28S and 5.8S rRNA sequences in interacting regions, the secondary structure that can be proposed for mouse complex is perfectly identical with yeast's, with all the 41 base-pairings between the two molecules maintained through 11 pairs of compensatory base changes. The other regions of the mouse 28S rRNA 5'terminal domain, which have extensively diverged in primary sequence, can nevertheless be folded in a secondary structure pattern highly reminiscent of their yeast' homolog. A minor revision is proposed for mouse 5.8S rRNA sequence.
Atropisomer Control in Macrocyclic Factor VIIa Inhibitors (2016)

Incorporation of a methyl group onto a macrocyclic FVIIa inhibitor improves potency 10-fold but is accompanied by atropisomerism due to restricted bond rotation in the macrocyclic structure, as demonstrated by NMR studies. We designed a conformational constraint favoring the desired atropisomer in which this methyl group interacts with the S2 pocket of FVIIa. A macrocyclic inhibitor incorporating this constraint was prepared and demonstrated by NMR to reside predominantly in the desired conformation. This modification improved potency 180-fold relative to the unsubstituted, racemic macrocycle and improved selectivity. An X-ray crystal structure of a closely related analogue in the FVIIa active site was obtained and matches the NMR and modeled conformations, confirming that this conformational constraint does indeed direct the methyl group into the S2 pocket as designed. The resulting rationally designed, conformationally stable template enables further optimization of these macrocyclic inhibitors.
Todos los pobres son delincuentes (2003)

Senalar la relacion que existe entre el incremento de la pobreza y el aumento en las tasas delictivas no significa afirmar que quien afronta carencias materiales necesariamente se dedicara a cometer transgredir la ley. Si bien ocurre, tal conducta no puede constituirse en categoria explicativa de la mencionada ligazon.
Security control for COTS components (1998)

Using COTS components poses serious threats to system security. The authors analyze the risks and describe how their sandbox method can limit the damage potential of COTS components. The sandbox model was originally developed for fault tolerance. Rather than eliminating actual failures, it provides a restricted environment to confine application behavior. The approach confines the damage caused if an application accidentally or maliciously misbehaves. The authors' sandbox method differs from Java's, in that it is built with OS support rather than with support from a particular language. The authors describe the Sendmail version of their sandbox method. Their approach requires B-level security features not found on most conventional OSs. Typically developed for government or military use, B-level certified OSs have more sophisticated security features. The authors explain that their method does not eliminate security problems but rather mitigates the damage caused by compromised applications and thus prevents most common security breaches. Untrusted COTS components can thus be safely plugged into a system without major reengineering, provided there is a suitable security platform.
Theoretical considerations of chemical reactions in micro-reactors operating under electroosmotic and electrophoretic control (1999)

P. D. I. Fletcher, S. J. Haswell and V. N. Paunov, Analyst, 1999, 124, 1273 DOI: 10.1039/A903624E
Classical and modern receiver architectures (2000)

This article is a review of several classical and modern wireless receiver architectures used in wideband wireless communication systems. The emphasis is on configurations suitable for integration on a single silicon chip. A full understanding of the design trade-offs discussed in this article is necessary for the proper introduction of a new modulation scheme presented in the article by Mirabbasi and Martin (2000) entitled, "Hierarchical QAM: a spectrally efficient DC-free modulation scheme".
Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproduction technologies (2012)

A possible association between ART and BWS/SRS was found, and we observed a more widespread disruption of genomic imprints after ART. The increased frequency of imprinting disorders after ART is perhaps not surprising given the major epigenetic events that take place during early development at a time when the epigenome is most vulnerable.
Fibrin sealant in bone transplantation: No effects on blood flow and bone formation in dogs (1986)

To study bone formation and regional blood flow following the use of fibrin sealant in autologous cancellous bone transplantation, a dog model was developed. In 18 dogs, a standardized defect in both tibiae was filled with an autologous iliac crest graft. On one side, the bone chips were mixed with fibrin sealant while the other side served as control. After 1, 2 and 3 weeks the blood flow of the transplant was calculated and the new bone formed evaluated histomorphometrically. Generally, the highest blood flow rates and most intensive new bone formation were observed at 2 weeks postoperatively. Fibrin sealant did not alter blood flow or new bone formation, but a tendency to diminished new bone formation was found in some grafts. Our study does not support the application of fibrin sealant in ordinary cancellous bone grafting.
1000 Genomes Project Promises Closer Look at Variation in Human Genome (2008)

took issue with the use of boarding and crowding as part of a management strategy. “The financial incentives are out of sync for a variety of services hospitals offer for patients,” Steinberg said. “Hospitals are trying a variety of ideas to address overcrowding, but there is no one-size-fits-all solution.” But some potential solutions are being considered. For example, hospitals could try to reduce the traditional use of the early days of the week for elective surgeries, which limits the number of inpatient beds available at that time. “I see 180 patients a day, and I can’t get them an inpatient bed on a Monday, but I see the same volume on a Friday and have no problems,” said Judd E. Hollander, MD, professor and clinical research director in the Department of Emergency Medicine at the University of Pennsylvania School of Medicine. Using the inpatient services of hospitals on weekends and the evening hours during the week could also ease boarding, Hollander added. Another possible solution to ease crowding is to move patients boarding in the emergency department to hallways of inpatient areas of the hospital. “Instead of having 20 emergency department beds being used, you have 2 patients in hallways on each floor of a 10-story hospital,” said Kellermann, who noted such a scheme is viewed cautiously by inpatient nurses who worry of being overburdened. “What one often finds is the ‘miracle of the elevator’—by the time a patient gets to the floor, there’s a clean bed waiting,” Kellerman said. However, an impediment to trying such solutions is a fear of hospital administrators that being the first and perhaps only institution to do so in a community could lead to that hospital being flooded with uninsured patients, which might overburden the institution financially. Still, said Pines, while one can appreciate a hospital’s desire to be financially stable, the institution must remember why it exists in the first place. “Usually a hospital’s mission statement calls for it to provide the best care to any patient regardless of status,” he said.
Growth without Postannealing of Monoclinic VO2 Thin Film by Atomic Layer Deposition Using VCl4 as Precursor (2018)

Vanadium dioxide (VO2) is a multifunctional material with semiconductor-to-metal transition (SMT) property. Organic vanadium compounds are usually employed as ALD precursors to grow VO2 films. However, the as-deposited films are reported to have amorphous structure with no significant SMT property, therefore a postannealing process is necessary for converting the amorphous VO2 to crystalline VO2. In this study, an inorganic vanadium tetrachloride (VCl4) is used as an ALD precursor for the first time to grow VO2 films. The VO2 film is directly crystallized and grown on the substrate without any postannealing process. The VO2 film displays significant SMT behavior, which is verified by temperature-dependent Raman spectrometer and four-point-probing system. The results demonstrate that the VCl4 is suitably employed as a new ALD precursor to grow crystallized VO2 films. It can be reasonably imagined that the VCl4 can also be used to grow various directly crystallized vanadium oxides by controlling the ALD-process parameters.
Mg2+-Selective Electrode Comprising Double-Helical DNA as Receptive Entity (1994)

Abstract Cyclic voltammograms of ferrocyanide/ferricyanide redox couple with a DNA-immobilized electrode gave the peak currents due to the reversible electrode reaction, which were significantly enhanced on adding Mg2+. The electrode responded also to Ca2+ and Ba2+, although the onset concentrations of the electrode response were 50-times larger than that for Mg2+. The selectivity in the order of Mg2+ &amp;gt; Ca2+, Ba2+ &amp;gt;&amp;gt; Na+, K+ seems consistent with the binding affinity of the metal ions with double-helical DNA.