gebhart/patrepeval huggingface dataset:
Dual effects of soluble CD14 on LPS priming of neutrophils (1997)

To evaluate the effect of soluble CD14 (sCD14) on human neutrophil response to lipopolysaccharide (LPS), we developed an LPS-priming assay that measures the chemiluminescence response to N-formyl-methionyl-leucyl-phenylalanine stimulation. Priming by 1 ng/mL rough LPS occurred in the presence of either serum or recombinant LPS-binding protein (LBP) only. Priming was completely CD14-dependent because preincubation of the neutrophils with an anti-CD14 monoclonal antibody prevented priming. We hypothesize that sCD14 enhances LPS response in neutrophils, but this response is not as effective as LPS response via membrane CD14 (mCD14). In our experiments sCD14 is present in an excess compared with mCD14. Priming of neutrophils occurs with low LBP, supposedly via sCD14-LPS complexes. With high LBP, addition of sCD14 inhibited LPS-priming of neutrophils. In that case, LPS may be transported to sCD14, preventing a more effective response via mCD14. In this study we demonstrate that the effect of sCD14 on neutrophil response to LPS is a delicate balance between activation and inhibition depending on concentration of serum or LBP.
Efficient methods for out-of-order load/store execution for high-performance soft processors (2013)

As FPGAs continue to increase in size, it becomes increasingly feasible and desirable to build higher performance soft processors. Preserving the familiar single-threaded programming model can be done with an out of order processor. The ability to execute memory loads and stores out of order has a large impact on performance, but this is difficult to do because the dependencies between stores and loads are not known until addresses are computed. Out of order memory disambiguation is traditionally done with CAMs in the load queue and store queue, but large CAMs are inefficient on FPGAs. Store Queue Index Prediction (SQIP) and NoSQ propose to replace CAMs with store-load forwarding prediction and load re-execution. We implement four memory disambiguation schemes (in-order, CAM, SQIP, NoSQ) on a Stratix IV FPGA and evaluate the area and delay trade-offs. We find that CAM area and delay degrade quickly with load/store queue size, while SQIP and NoSQ have little degradation with queue size but have area overhead for prediction and predictor training hardware. SQIP and NoSQ use less area than CAMs beyond 32 and 16 load/store queue entries, respectively, and have higher maximum frequency beyond 4 entries.
The impact of fluctuating workloads on well-being and the mediating role of work−nonwork interference in this relationship. (2013)

Heavy workloads have been central to the discussion of well-being. However, fluctuations in workloads have received sparse attention, even though transient and routine levels of workloads may have independent effects on well-being. The article assesses this, particularly focusing on the effort-recovery model of the workload/well-being relationship in which work-nonwork interference mediates this relationship. Using data from a weekly diary study of freelance or portfolio workers, multilevel analyses showed that both routine and transient levels of work to nonwork interference mediated the job demands-calmness relationship. Work to nonwork interference also negatively mediated the hours worked-calmness relationship, but the direct relationship between hours worked and calmness was a positive one so the mediating effect reduced this positive effect. In the case of enthusiasm, which was a second measure of well-being, there were no mediating effects but both routine and nonroutine levels of job demands and transient levels of hours worked were related to it. The study highlights the value of introducing the temporal dimension into the study of the work-nonwork interface, and the diversity of relationships that occur across different dimensions of workload and well-being.
Precise matching of 3-D target models to multisensor data (1997)

This paper presents a three-dimensional (3-D) model-based ATR algorithm that operates simultaneously on imagery from three heterogeneous, approximately boresight aligned sensors. An iterative search matches models to range and optical imagery by repeatedly predicting detectable features, measuring support for these features in the imagery, and adjusting the transformations relating the target to the sensors in order to improve the match. The result is a locally optimal and globally consistent set of 3-D transformations that precisely relate the best matching target features to combined range, IR, and color images. Results show the multisensor algorithm recovers 3-D target pose more accurately than does a traditional single-sensor algorithm. Errors in registration between images are also corrected during matching. The intended application is imaging from semiautonomous military scout vehicles.
Serious Technology Assessment for Health Care Information Technology (1997)

United States health care is engaged in an ambitious project to make its clinical and administrative records "100% electronic." Substantial benefits are expected in both clinical care delivery and medical research (especially for public health surveillance and outcomes/effectiveness studies). Substantial costs also potentially accrue, beyond the large outlays for an expanded computer and telecommunications infrastructure. Privacy and confidentiality are obviously at risk if such systems cannot be made secure. Limited empirical evidence currently available suggests health information systems security may not be very good, at least in the "average" institutional setting. Privacy-focused critics of electronic record-keeping are sometimes accused of taking Luddite stands, insufficiently attentive to IT's benefits. It may also be fair to worry about a certain Panglossian tendency in "industry" commentary, insufficiently attentive to potential problems. Better federal and state laws structuring health data use will help; the industry must also attend more candidly to the technical uncertainties.
Forskolin (2012)

Forskolin is the first pharmaceutical drug and product derived from a plant to be approved in India by the DCGI in 2006. Forskolin (7beta-acetoxy-8, 13-epoxy-1a, 6β, 9a-trihydroxy-labd-14-en-11-one) is a diterpenoid isolated from plant Coleus forskohlii (Lamiaceae). It is a lipid-soluble compound that can penetrate cell membranes and stimulates the enzyme adenylate cyclase which, in turn, stimulates ciliary epithelium to activate cyclic adenosine monophosphate, which decreases intraocular pressure (IOP) by reducing aqueous humor inflow. The topical application of forskolin is capable of reducing IOP in rabbits, monkeys, and humans. In its drug interactions, forskolin may act synergistically with epinephrine, ephedrine and pseudoephedrine. Whereas the effects of anti-clotting medications like warfarin, clopidogre, aspirin, anoxaparin, etc., may be enhanced by forskolin. Forskolin is contraindicated in the medications for people with ulcers as forskolin may increase acid level. Forskolin has a very good shelf-life of five years. Recently, its Ophthalmic inserts and in situ gels for sustained and delayed-release drug delivery systems were tested in New Zealand Albino Rabbits for its antiglaucoma efficacy. This drug review explains Forskolin as a drug, its antiglaucoma potential and recent findings of forskolin as an antiglaucoma agent. The literature search method used for this review was different databases and search engines like PubMed, International Pharmaceutical Abstracts, Google, Medicinal and Aromatic Plants (MAPA).
Expression ratio of CCND1 to CDKN2A mRNA predicts RB1 status of cultured cancer cell lines and clinical tumor samples (2011)

We report here that the CCND1/CDKN2A mRNA expression ratio predicts the RB1 status of cell lines in vitro and xenograft tumors and clinical tumor samples in vivo. Given the high predictive accuracy and quantitative nature of the CCND1/CDKN2A expression assay, the assay could be utilized to stratify patients for anti-tumor agents with preferential effects on either RB1-positive or -negative tumors.
Bacillus coagulansas a probiotic (2010)

Probiotics are live microbial feed additions that improve human or animal health. Their activities are towards improving the composition of the gastrointestinal microbiota in a manner that reduces the risk of disorder. In some cases, probiotics are also used therapeutically. Most probiotics use lactobacilli or bifidobacteria as the main constituents. These produce lactic acid as well as other anti-pathogenic attributes. Traditionally, probiotics are incorporated in dairy products (yoghurts or fermented drinks) or in lyophilised form. Because of stability and viability factors, heated products are not usually a target for probiotic use. This is because they are temperature sensitive. However, a spore-forming genus would have the ability to overcome this limitation. Here, we discuss evidence for the spore-forming Gram-positive bacterium Bacillus coagulans as a probiotic.
The bacterial Neo gene confers neomycin resistance to mammalian cochlear hair cells (1999)

The aminoglycoside antibiotics are important agents in the treatment of bacterial infection. At pharmacological doses they also preferentially damage the sensory hair cells of the inner ear, leading to ototoxic hearing loss. However, it has been suggested that the mechanism of ototoxicity is different from that of bacterial toxicity. The bacterial neomycin phosphotransferase gene (Neo) confers resistance to this and related aminoglycoside antibiotics. To determine whether the Neo gene also confers resistance to vertebrate ototoxicity, the sensitivity of cochlear hair cells to neomycin was evaluated in mice with a targeted insertion of Neo. Organotypic cultures of the organ of Corti, isolated from neonatal wild-type mice and two strains of mice carrying the Neo gene, were cultured for 72 h in the absence (controls) or in the presence of 200 microM neomycin. Organs from wild-type mice showed no remaining outer hair cells and <25% of inner hair cells when incubated with neomycin. In contrast, organs from mice carrying the Neo gene showed no loss of hair cells after neomycin treatment.
Utilization of Nerve Conduction Studies for the Diagnosis of Polyneuropathy in Patients with Diabetes: A Retrospective Analysis of a Large Patient Series (2008)

For over 70% of the patients, the specific diagnostic question of the presence of DPN was addressed by NCS with evidence-based criteria. The demographic features were strongly associated with risk of diabetes and DPN, suggesting that NCS were applied to appropriate demographic subgroups. The rate of DPN was also comparable to levels seen by academic electromyography laboratories. In 32.6% of the encounters the NCS suggested a posttest diagnosis other than DPN. This rate was similar to the results of referral to traditional electromyography laboratories. This study demonstrated that NCS using computer-based electrodiagnostic equipment was a suitable tool for the diagnosis of DPN. Furthermore, this technology permits examination of DPN in large populations.
The proapoptotic function ofDrosophilaHid is conserved in mammalian cells (1999)

Three genes-reaper, grim, and hid-are crucial to the regulation of programmed cell death in Drosophila melanogaster. Mutations involving all three genes virtually abolish apoptosis during development, and homozygous hid mutants die as embryos with extensive defects in apoptosis. Although Hid is central to apoptosis in Drosophila, it has no mammalian homologue identified to date. We present evidence that expression of Drosophila Hid in mammalian cells induces apoptosis. This activity is subject to regulation by inhibitors of mammalian cell death. We show that the N terminus of Hid, which is a region of homology with Reaper and Grim, is essential for Hid's function in mammalian cells. We demonstrate that Hid is localized to the mitochondria via a hydrophobic region at its C terminus and functionally interacts with BclXL. This study shows that the function of Hid as a death inducer in Drosophila is conserved in mammalian cells and argues for the existence of a mammalian homologue of this critical regulator of apoptosis.
A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids (2005)

Synthetic vectors based on reducible polycations consisting of histidine and polylysine residues (HIS RPCs) were evaluated for their ability to deliver nucleic acids. Initial experiments showed that RPC-based vectors with at least 70% histidine content mediated efficient levels of gene transfer without requirement for the endosomolytic agent chloroquine. Significant gene transfer was observed in a range of cell types achieving up to a 5-fold increase in the percentage of transfected cells compared to 25 kDa PEI, a gold standard synthetic vector. In contrast to 25 kDa PEI, HIS RPCs also mediated efficient transfer of other nucleic acids, including mRNA encoding green fluorescent protein in PC-3 cells and siRNA directed against the neurotrophin receptor p75(NTR) in post-mitotic cultures of rat dorsal root ganglion cell neurons. Experiments to elevate intracellular glutathione and linear profiling of cell images captured by multiphoton fluorescent microscopy highlighted that parameters such as the molecular weight and rate of cleavage of HIS RPCs were important factors in determining transfection activity. Altogether, these results demonstrate that HIS RPCs represent a novel and versatile type of vector that can be used for efficient cytoplasmic delivery of a broad range of nucleic acids. This should enable different or a combination of therapeutic strategies to be evaluated using a single type of polycation-based vector.
Magnetic coil design considerations for functional magnetic stimulation (2000)

Our studies have demonstrated effective stimulation of the bladder, bowel, and expiratory muscles in patients with spinal cord injury using functional magnetic stimulation. However, one limitation of the magnetic coils (MC) is related to their inability to specifically stimulate the target tissue without activation of surrounding tissue. The primary goal of this study was to determine the governing parameters in the MC design, such as coil configuration, diameter, and number of turns in one loop of the coil. By varying these parameters, our approach was to design, construct, and evaluate the induced electric field distributions of two sets of novel MC's. Based on the slinky coil design, the first set of coils was constructed to compare their abilities in generating induced electric fields for focal nerve excitation. The second set of coils was built to determine the effect that changes in two parameters, coil diameter and number of turns in one loop, had on field penetration. The results showed that the slinky coil design produced more focalized stimulation when compared to the planar round coils. The primary-to-secondary peak ratios of the induced electric field from slinky 1 to 5 were 1.00, 2.20, 2.85, 2.62, and 3.54. We also determined that coils with larger diameters had better penetration than those with smaller diameters. Coils with less number of turns in one loop had higher initial field strengths; when compared to coils that had more turns per loop, initial field strengths remained higher as distance from the coil increased. In our attempt to customize MC design according to each functional magnetic stimulation application and patients of different sizes, the parameters of MC explored in this study may facilitate designing an optimal MC for a certain clinical application.
TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS (2003)

In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of\nparasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene\nlaid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was\nconstructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first\nand second days post-transfection，clear positive fluorescences were detected in some parasite bodies，\nindicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However，\nthe positive parasites were not ubiquitous and\nfinally disappeared until the forth day post-transfection. This is\nthe first time that the expression of a foreign gene has been reported in the Babesia parasite.
Hematopoietic effects and clinical uses of granulocyte-macrophage colony-stimulating factor and PIXY321. (1994)

The clinical uses of granulocyte-macrophage colony-stimulating factor (GM-CSF) are expanding. GM-CSF was originally approved for use after autologous bone marrow transplantation. More recent phase III studies using GM-CSF after allogenic marrow transplantation show a similar reduction in the duration of neutropenia and monocytopenia. In phase III trials following standard-dose chemotherapy, GM-CSF significantly reduced treatment-related neutropenia and morbidity and, in one study, mortality. Nonrandomized studies using GM-CSF prior to and during chemotherapy for acute myeloid leukemia to improve remission rates have not demonstrated an obviously significant benefit. Other studies of GM-CSF to augment cancer therapies directly and to aid in the treatment of infection are in progress. PIXY321 is a fusion protein of GM-CSF and interleukin-3. PIXY321 was developed, in part, because in vitro and in vivo laboratory studies suggested synergy between its two components. Phase I-II clinical studies using PIXY321 are preliminary but show encouraging results with stimulation of multilineage hematopoiesis.
The Methylosome, a 20S Complex Containing JBP1 and pICln, Produces Dimethylarginine-Modified Sm Proteins (2001)

snRNPs, integral components of the pre-mRNA splicing machinery, consist of seven Sm proteins which assemble in the cytoplasm as a ring structure on the snRNAs U1, U2, U4, and U5. The survival motor neuron (SMN) protein, the spinal muscular atrophy disease gene product, is crucial for snRNP core particle assembly in vivo. SMN binds preferentially and directly to the symmetrical dimethylarginine (sDMA)-modified arginine- and glycine-rich (RG-rich) domains of SmD1 and SmD3. We found that the unmodified, but not the sDMA-modified, RG domains of SmD1 and SmD3 associate with a 20S methyltransferase complex, termed the methylosome, that contains the methyltransferase JBP1 and a JBP1-interacting protein, pICln. JBP1 binds SmD1 and SmD3 via their RG domains, while pICln binds the Sm domains. JBP1 produces sDMAs in the RG domain-containing Sm proteins. We further demonstrate the existence of a 6S complex that contains pICln, SmD1, and SmD3 but not JBP1. SmD3 from the methylosome, but not that from the 6S complex, can be transferred to the SMN complex in vitro. Together with previous results, these data indicate that methylation of Sm proteins by the methylosome directs Sm proteins to the SMN complex for assembly into snRNP core particles and suggest that the methylosome can regulate snRNP assembly.
Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option (2013)

The glow discharge from a novel spark-based nonthermal plasma generator is described and characterized using spectrophotometry, radiometry and gas detectors. Spectral information identified ultraviolet radiation within the plasma discharge. High levels of reactive oxygen and nitrogenous species were also detected within the analyzed plasma. The plasma discharge is found to have a significant germicidal effect on Gram-positive bacteria, independent of temperature increase. The ultraviolet radiation and ionized gaseous species are found to contribute to this observed effect, synergistically. Plasma filtration reduced germicidal survivability significantly. The liquid environment in which bacterial cells were exposed to the nonthermal plasma also was important, when determining the efficacy of nonthermal plasma, as a germicidal agent. Staphylococcus aureus cultures exposed to nonthermal plasma for two minutes within low nutrient sources, such as water or phosphate-buffered saline, had very low survival. Cultures exposed to the same plasma interval in nutrient rich liquids tolerated the plasma exposure better, and survived at higher numbers. This plasma discharge is highly bactericidal, due to the high concentrations of known antimicrobial agents, but its effectiveness is subject to a synergistic effect, that depends upon the surrounding environment.
Using Wireless Power Meters to Measure Energy Use of Miscellaneous and Electronic Devices in Buildings (2012)

E RNEST O RLANDO L AWRENCE B ERKELEY N ATIONAL L ABORATORY Using Wireless Power Meters to Measure Energy Use of Miscellaneous and Electronic Devices in Buildings Richard Brown 1 , Steven Lanzisera 1 , Hoi Ying (Iris) Cheung 1 , Judy Lai 1 , Xiaofan Jiang 2 , Stephen Dawson-Haggerty 2 , Jay Taneja 2 , Jorge Ortiz 2 , David Culler 2 Lawrence Berkeley National Laboratory, Berkeley, California, USA University of California, Berkeley, USA Environmental Energy Technologies Division May 2011 Published in the Proceedings of the Energy Efficiency in Domestic Appliances and Lighting (EEDAL) 2011 Conference, Copenhagen, Denmark, May 24-26, 2011 This work was supported by the Assistant Secretary for Energy Efficiency and Renewable Energy, Office of Technology Development, Building Technologies Program, of the U.S. Department of Energy under Contract No. DE-AC02- 05CH11231.
Scratch resistant polyimide coatings for aluminosilicate glass surfaces (2002)

Several polyimide resins were tested as possible scratch resistant coatings for aluminosilicate glass centrifuge tubes. Tubes with siloxane pretreatments provided the best adhesion between the polyimide and glass surface. Resins synthesized with an alkoxysilane group incorporated into the polyimide chain also showed improved adhesion, but the results were not as significant as when the pretreatment was used. Elastic recovery and effective Young's modulus of the polyimide coatings were calculated from nano-scratching. The results indicate that polyimides with a lower modulus, and higher elastic recovery, protected the glass surface best, exhibiting compression of the coating layer. An example was poly-4-4′-oxydiphenylene pyromellitimide (PMDA-ODA), whereas the coatings with a high effective Young's modulus and low elastic recovery ruptured. An example of a failed coating was poly-4,4′-carbonyldiphenylene 3,3′,4,4′-biphenylenetetracarboximide (BPDA-DABP) which had low amount of elastic recovery, high effective Young's modulus and a large amount of flaking during macroscratch testing.
Definition of a minimal optimal cytotoxic T-cell epitope within the hepatitis B virus nucleocapsid protein (1993)

Residues 11 to 27 of the hepatitis B virus nucleocapsid antigen contain a cytotoxic T-cell epitope that is recognized by cytotoxic T cells from virtually all HLA-A2-positive patients with acute hepatitis B virus infection. Using panels of truncated and overlapping peptides, we now show that the optimal amino acid sequence recognized by cytotoxic T cells is a 10-mer (residues 18 to 27) containing the predicted peptide-binding motif for HLA-A2 and that this peptide can stimulate cytotoxic T cells able to recognize endogenously synthesized hepatitis B core antigen. Since patients with chronic hepatitis B virus infection fail to mount an efficient cytotoxic T-cell response to it, this epitope might serve as the starting point for the design of synthetic peptide-based immunotherapeutic strategies to terminate persistent viral infection.