gebhart/patrepeval huggingface dataset:
Aberrant Expression of Cyr61, a Member of the CCN (CTGF/Cyr61/Cef10/NOVH) Family, and Dysregulation by 17β-Estradiol and Basic Fibroblast Growth Factor in Human Uterine Leiomyomas (2001)

Uterine leiomyomas are the most common tumors of the reproductive tract, afflicting women between the ages of 30--55 yr. Although considered to be the leading cause of hysterectomies in the United States, little is known of the etiology and mechanisms of pathogenesis in leiomyomas. Accordingly, rapid analysis of differential expression (RADE) was employed to identify genes that are abnormally expressed in leiomyomas. Of the several genes identified, Cyr61, a member of the CCN family of growth and angiogenic regulators, was shown to be markedly down-regulated at the messenger ribonucleic acid (mRNA) and protein levels in leiomyoma tumors compared with the matched uterine myometrial controls (n = 38). In addition, in situ hybridization experiments corroborated the lack of Cyr61 expression in leiomyoma cells, whereas abundant transcript levels were identified in adjacent myometrial smooth muscle cells. To elucidate the mechanisms of Cyr61 gene regulation in leiomyomas, we determined the effects of ovarian steroids, basic fibroblast growth factor (bFGF), and serum, on Cyr61 expression using an ex vivo culture system. Treatment of human myometrial explants with 17 beta-estradiol and bFGF up-regulated Cyr61 transcripts, whereas the progesterone receptor agonist, R5020 (alone or in combination with 17 beta-estradiol), had no effect. Paradoxically, neither 17 beta-estradiol nor bFGF was capable of up-regulating Cyr61 mRNA in leiomyoma explants despite elevated levels of ER alpha mRNA, suggesting a possible defect in steroid and growth factor regulation. Thus, dysregulation of Cyr61 by estrogen and bFGF may contribute to down-regulation of Cyr61 in leiomyomas, which, in turn, may predispose uterine smooth muscle cells toward sustained growth.
Detection of (11; 22)(q24;q12) Translocation-Bearing Cells in Peripheral Blood Progenitor Cells of Patients With Ewing's Sarcoma Family of Tumors (1995)

Journal Article Detection of (11; 22)(q24;q12) Translocation-Bearing Cells in Peripheral Blood Progenitor Cells of Patients With Ewing's Sarcoma Family of Tumors Get access Jeffrey A. Toretsky, Jeffrey A. Toretsky Pediatric Branch, Division of Cancer Treatment National Cancer InstituteBethesda, Md Search for other works by this author on: Oxford Academic PubMed Google Scholar Leonard Neckers, Leonard Neckers Clinical Pharmacology Branch, Division of Cancer Treatment National Cancer InstituteBethesda, Md. Search for other works by this author on: Oxford Academic PubMed Google Scholar Leonard H. Wexler Leonard H. Wexler Pediatric Branch, Division of Cancer Treatment National Cancer InstituteBethesda, Md Leonard H. Wexler, M.D., Pediatric Branch, Bldg. 10 Rm. 13N240. 9000 Rockville Pike, Bethesda, MD 20892. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 87, Issue 5, 1 March 1995, Pages 385–386, https://doi.org/10.1093/jnci/87.5.385 Published: 01 March 1995 Article history Received: 26 July 1994 Revision received: 20 December 1994 Accepted: 27 December 1994 Published: 01 March 1995
Pharmacokinetics and Mechanism of Action of a Doxorubicin-Monoclonal Antibody 9.2.27 Conjugate Directed to a Human Melanoma Proteoglycan (1988)

Doxorubicin (DXR) conjugated to a monoclonal antibody (MAb), 9.2.27, which recognizes a human melanoma-associated proteoglycan, effectively suppresses the growth of human melanoma xenografts and prolongs the life span of tumor-bearing athymic nude (nu/nu) mice. We have investigated further the mechanism(s) of this in vivo antitumor activity. Our results indicate that following iv injection, the DXR-MAb 9.2.27 conjugate is cleared from the circulation with typical biphasic kinetics, similar to the clearance process of the unconjugated MAb 9.2.27. In contrast, less than 10% of injected dose per milliliter of blood is found in the circulation at any given time after ip injection. Toxicity studies further indicate that DXR-MAb 9.2.27 conjugate is less toxic in vivo than the freely administered DXR, which is known to cause considerable cardiotoxic effects. Direct autoradiography demonstrates that the DXR-MAb 9.2.27 conjugate binds specifically to the tissue sections derived from a human melanoma xenograft of a nude mouse. A critical evaluation is given of the relevance of these findings and their impact on the design of future strategies for the immunochemotherapy of malignant melanoma.
PAI‐1 in tissue fibrosis (2011)

Fibrosis is defined as a fibroproliferative or abnormal fibroblast activation-related disease. Deregulation of wound healing leads to hyperactivation of fibroblasts and excessive accumulation of extracellular matrix (ECM) proteins in the wound area, the pathological manifestation of fibrosis. The accumulation of excessive levels of collagen in the ECM depends on two factors: an increased rate of collagen synthesis and or decreased rate of collagen degradation by cellular proteolytic activities. The urokinase/tissue type plasminogen activator (uPA/tPA) and plasmin play significant roles in the cellular proteolytic degradation of ECM proteins and the maintenance of tissue homeostasis. The activities of uPA/tPA/plasmin and plasmin-dependent MMPs rely mostly on the activity of a potent inhibitor of uPA/tPA, plasminogen activator inhibitor-1 (PAI-1). Under normal physiologic conditions, PAI-1 controls the activities of uPA/tPA/plasmin/MMP proteolytic activities and thus maintains the tissue homeostasis. During wound healing, elevated levels of PAI-1 inhibit uPA/tPA/plasmin and plasmin-dependent MMP activities, and, thus, help expedite wound healing. In contrast to this scenario, under pathologic conditions, excessive PAI-1 contributes to excessive accumulation of collagen and other ECM protein in the wound area, and thus preserves scarring. While the level of PAI-1 is significantly elevated in fibrotic tissues, lack of PAI-1 protects different organs from fibrosis in response to injury-related profibrotic signals. Thus, PAI-1 is implicated in the pathology of fibrosis in different organs including the heart, lung, kidney, liver, and skin. Paradoxically, PAI-1 deficiency promotes spontaneous cardiac-selective fibrosis. In this review, we discuss the significance of PAI-1 in the pathogenesis of fibrosis in multiple organs.
Genomic data for alternate production strategies. I. Identification of major contaminating species for Cobalt+2 immobilized metal affinity chromatography (2004)

Recent advances in technology have allowed for the identification of complex protein mixtures in a rapid fashion. This report highlights the use of 2D gel electrophoresis, mass spectrometry, and database analysis to determine contaminating species of the Escherichia coli genome that are present during immobilized metal affinity chromatography (IMAC), highlighting Co(2+) as the affinity ligand. Four proteins (triosephosphate isomerase, alpha galactosidase, Hsp90, and glucosamine 6-phosphate synthase) constitute the majority of E. coli proteins that bind and potentially may coelute during chromatography. Results are discussed within the context of changes that when implemented could lead to an increase in IMAC efficiency, not by altering column conditions, but rather by changing the nature of the nuisance proteins that principally reduce column capacity and extend processing times. Such a study illustrates the use of proteome data to aid in bioprocess design.
Helical computed tomography at 1.5:1 pitch reconstructed at 15-mm and 7-mm intervals for examination of patients with suspected metastatic disease. (1996)

Metastatic lesions may be missed by helical CT at 1.5:1 pitch if overlapped reconstruction is not performed.
Epidemiologic Studies of Risk Factors for Cancer in Pet Dogs (1998)

In 1996, an estimated 52.9 million dogs were living in 31.6 percent of all households in the United States (1). Several epidemiologic studies have been undertaken to identify risk factors for cancer in pet dogs. This presentation reviews the literature on canine cancer epidemiology with a view towards exploring how these studies inform us about cancer causation in dogs and in humans. It focuses on observational studies of dogs living in their usual environments, and considers experimental studies in laboratory settings only to the extent that such studies shed light on the observational studies. It begins with a discussion of some methodological issues that make epidemiologic studies of cancer in dogs somewhat different from those in humans.
APFiLoc: An Infrastructure-Free Indoor Localization Method Fusing Smartphone Inertial Sensors, Landmarks and Map Information (2015)

The utility and adoption of indoor localization applications have been limited due to the complex nature of the physical environment combined with an increasing requirement for more robust localization performance. Existing solutions to this problem are either too expensive or too dependent on infrastructure such as Wi-Fi access points. To address this problem, we propose APFiLoc-a low cost, smartphone-based framework for indoor localization. The key idea behind this framework is to obtain landmarks within the environment and to use the augmented particle filter to fuse them with measurements from smartphone sensors and map information. A clustering method based on distance constraints is developed to detect organic landmarks in an unsupervised way, and the least square support vector machine is used to classify seed landmarks. A series of real-world experiments were conducted in complex environments including multiple floors and the results show APFiLoc can achieve 80% accuracy (phone in the hand) and around 70% accuracy (phone in the pocket) of the error less than 2 m error without the assistance of infrastructure like Wi-Fi access points.
Efficacy of Oxcarbazepine in the Treatment of Painful Diabetic Neuropathy (2004)

The results suggest that oxcarbazepine administered as monotherapy is an efficacious and safe option for the symptomatic treatment of pain associated with symmetrical diabetic neuropathy. These results will need to be confirmed in large, double-blind, placebo-controlled, randomized clinical trials.
Stability and Activity in Sputum of G10KHc, a Potent Anti‐Pseudomonas Antimicrobial Peptide (2007)

G10KHc, a specifically targeted antimicrobial peptide developed in our laboratory, has shown rapid and selective killing activity against Pseudomonas aeruginosa in culture medium. Because of the major role played by this pathogen in cystic fibrosis, we sought to evaluate the utility of G10KHc under more physiologic conditions in vitro. In the current study, we found that robust G10KHc activity could be maintained in expectorated sputum if serine protease-dependent digestion associated with this fluid was inhibited, either by chemical antagonists or by the construction of a D-amino acid enantiomer of G10KHc. Further investigations revealed that specifically targeted antimicrobial peptide activity in sputum could be further enhanced when samples were treated with a combination of peptide and recombinant human DNase. Our results illustrate the importance of investigating combination therapy to treat cystic fibrosis, especially if protease-sensitive peptide-based agents, such as G10KHc, are to be developed as alternatives to, or in conjunction with, conventional small-molecule antibiotics.
Mechano and Thermoluminescence of Gamma-irradiated CaSO4:Dy Phosphor (2002)

In order to have a better idea of the interaction of the defect centres produced by y-irradiation with dislocation in the processes of deformation destruction, mechanoluminescence (ML) and thermoluminescence (TL) of gamma-irradiated CaSO4:Dy, these phosphors have been investigated. CaSO4:Dy phosphors were prepared by dissolving CaSO4.2H2O in sulphuric acid and evaporating the excess acid around 300 degrees C. ML was excited impulsively by dropping a load on to the sample. Two peaks have been observed in the ML intensity against time curve. The total light output, i.e. integrated ML intensity, increases with concentration of dopant, strain rate and with irradiation doses. The TL glow curves of CaSO4:Dy phosphors at different concentrations of dopant and irradiation doses were also recorded. Studies of the influence of post-irradiation annealing on the ML of CaSO4:Dy show that with the removal of the TL dosemetric peak (approximately 210 degrees C) the ML intensity decreases markedly. A spectroscopic study of ML and TL has also been carried out to elucidate the mechanism of ML. Correlation between ML and TL has also been found.
Abstract 4410: Neutralizing Endosialin antibody, MORAb-004, enhances efficacy of TAXOTERE in a metastatic melanoma model (2012)

Abstract Endosialin/Tumor Endothelial Marker-1 (TEM1) is a cell surface glycoprotein that is expressed on cells involved in the development of tumor vasculature, primarily pericytes and stromal fibroblasts. Studies have found it to play a key role in tumor growth and neo-vessel formation in numerous cancer types. We are currently evaluating the safety and therapeutic potential of MORAb-004, a humanized monoclonal IgG targeting endosialin/TEM-1, in clinical trials. Because MORAb-004 only recognizes human antigen and has no cross-reactivity with rodent endosialin/TEM-1, we generated a human endosialin/TEM-1 knock-in mouse in order to assess its activity in vivo. Previously, in this transgenic model, we found that MORAb-004 significantly inhibited subcutaneous B16-F10 melanoma progression by ∼70% when administered intravenously for 5 consecutive days. Moreover, using the Lewis Lung Carcinoma model of metastatic disease our results showed that MORAb-004 had a significant inhibitory effect on the number of lung metastases. For these studies, supporting clinical trials for using MORAb-004 in combination with standard of care chemotherapeutics, we developed a B16-F10 melanoma lung colonization model. In this model, the human endosialin/TEM-1 knock-in mice were implanted intravenously with B16F10 cells adapted for in vivo lung colonization. These mice were treated with Docetaxel, MORAb-004, or the combination of the two agents, respectively. A group of the tumor-bearing mice receiving PBS served as control. At the end of the study, the lungs of each mouse were harvested. A ratio of the area of tumor nodules versus total lung surface area is calculated as tumor burden. MORAb-004, at 50 mg/kg three times weekly for a total of 7 i.v. injections reduced tumor burden by 45%. Treatment of Docetaxel, at 3mg/kg twice weekly for a total of 5 i.p. injections, reduced less than 20% of tumor burden in the lungs. When MORAb004 was added to Docetaxel treatment, a significant enhancement of efficacy was observed. The reduction of tumor nodules in the lungs was increased to approximately 70%. A similar trend is seen in combination therapy where MORAb-004 was added to gemcitabine regimen. Our results demonstrate the ability of MORAb-004 to inhibit tumor growth in a murine model. Moreover, when added to the chemotherapeutic regimen, it significantly enhances the therapeutic effect of traditional chemotherapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 4410. doi:1538-7445.AM2012-4410
ChemInform Abstract: Novel Synthesis of Pyrido(1,2‐a)benzimidazoles via Reaction of N‐Acyl Arylhydroxylamines with Pyridine. (1987)

Abstract The chlorodinitrobenzonitrile (I) is selectively reduced by catalytic hydrogenation or with TiCl 3 to produce the hydroxylamines (II) ‐ (IV) or the diamine (V).
Performance of Transcription-Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods (1998)

Based on the amplification of chlamydia-specific rRNA sequences and the ligase chain reaction (LCR), the performance characteristics of the Gen-Probe Chlamydia trachomatis transcription-mediated amplification (TMA) assay were evaluated with endocervical, urine, and vulval specimens from women and urethral and urine specimens from men and were compared with those for cultures on endocervical, vulval, and urethral swabs. Of the 308 women and 240 men tested, 25 (8.1%) and 44 (18.3%), respectively, were shown to be infected. By using the infected individual as the expanded "gold standard" for calculations, the TMA assay and LCR gave similar performances for the sensitivity of male urethral (93.2%) and urine (88.6 and 86.4%) samples, while culture detected only half of the 44 infected men. In women, the sensitivities of the TMA assay for endocervical and vulval samples were 88 and 92%, respectively, compared to values of 92% for the LCR on both sample types and of 52 and 8%, respectively, for culture. By using first-void urine for chlamydial diagnosis in women, LCR detected 24 (96%) and TMA assay detected 19 (76%) infected individuals, showing a significantly lower sensitivity for urine in women (P = 0.0253). The results indicate a high overall agreement for both amplifying techniques for all examined specimen types, except for female urine. Furthermore, they confirm the previous observation that vulval swabs are an effective alternative noninvasive sample type for the detection of C. trachomatis infection in women by nucleic acid-based amplification technologies.
Application of live attenuated measles and mumps vaccines in children with acute leukemia. (1981)

Eight children with acute leukemia in remission were immunized with live attenuated measles vaccine and 4 with mumps vaccine. Immunological examinations before vaccination showed that the numbers of white blood cells, percentages of lymphocytes, levels of immunoglobulins and responses to skin tests with DNCB, PPD, PHA and varicella antigen were within normal ranges in most of these children. Chemotherapy against leukemia was stopped for 2 weeks, from one week before to one week after vaccination. One child had a transient fever 10 days after measles vaccination, but no side reactions were observed in the others. Seroconversion was observed in all but one child who received measles vaccine, and neutralizing antibodies have persisted for at least 4 weeks and at most 3 years, when this study was terminated.
Detection of Molecular Monolayers by Optical Second-Harmonic Generation (1981)

Second-harmonic generation is shown to be sensitive enough to detect molecular monolayers adsorbed on a silver surface. Adsorption of AgCl and pyridine on silver during and after an electrolytic cycle can be easily observed.
PACS mini refresher course. Local area network topologies, media, and routing. (1992)

Picture archiving and communication systems (PACS) use local area networks (LANs) for the transfer of images and image-related data. There are several aspects of LANs that are used to describe and categorize them: topology, the media used for transmission, and the medium access control (MAC) protocols. The four basic topologies are the bus, tree, ring, and star; the four primary types of media used for LANs include coaxial cable, twisted-pair wire, fiberoptic cables, and wireless. The wireless LANs that use infrared and radio frequencies are new but promising. Of the three MACs presently being used--Ethernet, fiber distributed data interface (FDDI), and UltraNet--Ethernet is the most common. LANs can be interconnected by means of the repeater, bridge, router, and gateway internetworking devices. The future of LAN development lies in increased standardization and increased connectivity in order to make teleradiology a reality.
A review of screening tests for cognitive impairment (2006)

The merit of screening for dementia and cognitive impairment has been the subject of recent debate. One of the main limitations in this regard is the lack of robust evidence to support the many screening tests available. Although plentiful in number, few such instruments have been well validated in the populations for which they are intended to be used. In addition, it is likely that "one size does not fit all" in cognitive screening, leading to the development of many specialised tests for particular types of impairment. In this review, we sought to ascertain the number of screening tools currently available, and to examine the evidence for their validity in detecting different diagnoses in a variety of populations. A further consideration was whether each screen elicited indices of a range of cognitive, affective and functional domains or abilities, as such information is a valuable adjunct to simple cut-off scores. Thirty-nine screens were identified and discussed with reference to three purposes: brief assessment in the doctor's office; large scale community screening programmes; and identifying profiles of impairment across different cognitive, psychiatric and functional domains/abilities, to guide differential diagnosis and further assessment. A small number of screens rated highly for both validity and content. This review is intended to serve as an evaluative resource, to guide clinicians and researchers in choosing among the wide range of screens which are currently available.
ISOLATION OF THE REGULATORY REGIONS AND GENOMIC ORGANIZATION OF THE PORCINE ??1,3-GALACTOSYLTRANSFERASE GENE1 (2000)

These observations have important implications for experiments involving genetic manipulation of the alpha1,3GT gene in transgenic animals in terms of promoter utilization, and particularly in genetically engineering cells for the animal cloning technology by nuclear transfer.
MANUAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY (1999)

The Culture The Process Strain Improvement Immobilization and Cell Culture Biochemical Engineering Special Topics
Contribution of Na+-glucose cotransport to the short-circuit current in the pigmented rabbit conjunctiva (1996)

The objective of this study was to determine whether Na(+)-glucose cotransport contributed to the residual short-circuit current (Isc) in the pigmented rabbit conjunctiva not accounted for by Cl- secretion. The Isc of the pigmented rabbit conjunctiva was measured following exposure of its mucosal or serosal side to varying concentrations of D-glucose or phloridzin in a glutathione-bicarbonated Ringer's solution (GBR). Addition of D-glucose to the mucosal side of the conjunctiva bathed in glucose-free GBR elevated Isc up to 26 +/- 6% in a dose-dependent manner (K0.5 = 1.2 mM), while mucosal (but not serosal) addition of phloridzin reduced the Isc (IC50 = 0.05 mM) of the conjunctiva bathed in regular GBR. In a mucosal Na(+)-free medium, neither 20 mM D-glucose nor 0.5 mM phloridzin was effective. In a mucosal glucose-containing medium, deletion of Na+ reduced Isc up to 27 +/- 4%. It was, however, restored to its initial value upon the addition of 17.5-141 mM Na+ to a medium containing 5 mM D-glucose. Hill plot analysis of Isc elevation at 141 mM Na+ in the presence of varying D-glucose concentrations in the mucosal bathing fluid yielded a Hill coefficient of 0.86. There is, therefore, evidence for the apical localization of phloridzin-sensitive Na(+)-coupled D-glucose transport that exhibits apparent 1:1 stoichiometry, being responsible for the maximal 26% increase in the Isc.