gebhart/patrepeval huggingface dataset:
Immunotherapy with monomethoxypolyethylene glycol modified allergens. (1990)

On the basis of the results of experiments in naive mice, i.e., in mice which had not been presensitized, it was anticipated that mPEG-modified allergens would suppress the specific IgE response in allergic humans. However, only minor or no suppression of IgE antibodies was induced on administration of mPEG conjugates in allergic patients with a longstanding IgE response. This observation was later confirmed in pre-sensitized animals. The mPEG-modified allergens can be synthesized in a reproducible manner. This makes mPEG-modified allergens suitable for production and quality control. There is indirect evidence, i.e., identification of IgE and IgG antibody stimulation against all identified allergens, that the relevant determinants are available for processing by the immune system. By contrast, the polymerization of allergens by formaldehyde or glutaraldehyde leads to ill-defined, cross-linked high molecular weight materials. As in the case of other modified allergens, mPEG-modified allergens have been developed primarily because of their lower allergenicity than the original allergen preparations. However, they also retain clinical efficacy of the same magnitude as that induced by unmodified allergen preparations, which is a prerequisite for clinical use. IT with mPEG-modified pollen allergen preparations has been proven to be as effective as IT with unmodified allergens, when these preparations are given in similar doses. Furthermore, a more pronounced beneficial effect can be obtained with higher doses of mPEG-modified allergen, without the risk of side effects which are often precipitated by unmodified allergens. Therefore, it seems reasonable to suggest that children and young adults with developing sensitivity to perennial allergens, and without chronic changes of the bronchial mucosa, are the most suitable candidates for IT with mPEG-modified allergen preparations. Mite asthma, which always includes bronchial inflammation and nonspecific hyperresponsiveness, represents a more complicated model. In mite asthmatics mPEG-modified mite allergen preparations were safer than the corresponding unmodified preparation. Although bronchial sensitivity to allergen and histamine did not change significantly in mite asthmatics during the observation time, it appears that IT with mPEG-modified mite allergen led to a decrease in the releasability of histamine from skin mast cells, as measured by SPT, to a similar degree as observed in pollinosis patients. Therefore, it may be inferred that the allergic component of asthma might be influenced by IT with mPEG allergens. As a consequence, before the start of IT, the degree of reversibility of pathophysiological changes in the bronchial wall should be considered.(ABSTRACT TRUNCATED AT 400 WORDS)
Long-Term Cardiac pro-B-Type Natriuretic Peptide Gene Delivery Prevents the Development of Hypertensive Heart Disease in Spontaneously Hypertensive Rats (2011)

AAV9 vector facilitates sustained cardiac proBNP overexpression and improves LV function in hypertensive heart disease. Long-term proBNP delivery improved both systolic and diastolic function. The effects on cardiac structure and function occurred independently of BP-lowering effects in normal Wistar rats.
L-myc cooperates with ras to transform primary rat embryo fibroblasts. (1988)

Recent molecular analysis has revealed that L-myc has several domains of extremely conserved amino acid sequence homology with c-myc and N-myc, suggesting similarity of function. We tested the biologic activity of L-myc by using an expression vector containing a cDNA clone coding for the major open reading frame in the 3.9-kilobase mRNA of L-myc under the control of a strong promoter (Moloney long terminal repeat) and found that L-myc complemented an activated ras gene in transforming primary rat embryo fibroblasts. However, the efficiency of transformation was 1 to 10% of that seen with the c-myc and simian virus 40 (SV40) controls. The L-myc/ras transformants initially grew more slowly than c-myc or SV40 transformants, but once established as continuous cell lines, they were indistinguishable from cell lines derived from c-myc/ras or SV40/ras transfectants as determined by morphology, soft-agar cloning, and tumorigenicity in nude mice.
Blending Images for Texturing 3D Models (2002)

This paper describes a novel system for building seamless texture maps for a surface of arbitrary topology from real images of the object taken with a standard digital camera and uncontrolled lighting. In our application we wish to take a sparse set of real images of a 3D object, and apply the images to an approximate surface model of the object to generate a high quality textured model. In practice the measured colour and intensity for a surface element observed in different photographic images will not agree. This is due to the interaction between real world lighting effects (such as highlights and specularities) and variations in the camera gain settings as well as registration and surface modelling errors. We describe a new automatic approach that extends a classical 2D image blending technique to a 3D surface, which produces high quality photo-realistic results at a low computational cost.
Effects of Rigid Contact Lens Edge Lift Changes on Tear Pump Efficiency (1991)

The effects of rigid contact lens axial edge lift changes on corneal oxygenation and tear exchange with the blink were studied for lenses of five axial edge lifts (0.05 to 0.13 mm in 0.02-mm steps). The overall and optic zone diameters were maintained at 8.8 and 7.4 mm, respectively, and all other parameters were held constant. Oxygen uptake rates were measured for the normal open eye after 5 min of static (without blinking) and dynamic (with blinking once every 5 s) contact lens wear. As expected, no significant differences were found in the oxygen uptake rates associated with the static wear of the contact lenses; however, under dynamic conditions, increasing edge lift was associated with a reduction in oxygen demand. The differences between data obtained under static and dynamic conditions increased with increasing axial edge lift, and subjects with small palpebral aperture sizes seemed to benefit more from increasing axial edge lift.
Partially structured self-associating states of acidic fibroblast growth factor (1993)

A combination of near- and far-UV circular dichroism, Fourier-transform infrared spectroscopy, tryptophan fluorescence, size-exclusion chromatography, and a fluorescent extrinsic hydrophobic probe has been employed to characterize partially structured states of human recombinant acidic fibroblast growth factor (aFGF). At low pH, the addition of specific polyanionic ligands or moderate amounts of salts induces states with high secondary but low tertiary structure content. At neutral pH, intermediate amounts of chaotropic agents impose similar partially structured conformational states which also display noncooperative unfolding transitions. Kinetic evidence indicates that similar forms of the protein exist in the first few hundred milliseconds in the refolding pathway of aFGF. The kinetics of their formation appear to be temperature-independent, implying lack of an energy barrier, which is characteristic for further slow folding into the native state. Unlike the native and fully unfolded states, these partially structured conformations exhibit very low solubility, resulting in irreversible aggregation. Potential physiological implications of the existence of such "molten globule" states with regard to the growth factor's transport and biological activity are considered.
Effect of preventive ( blocker) treatment, behavioural migraine management, or their combination on outcomes of optimised acute treatment in frequent migraine: randomised controlled trial (2010)

Clinical trials NCT00910689.
A novel approach to the generation of high affinity class II-binding peptides. (1990)

We have found that if core regions crucial for class II binding are incorporated in multiple copies in the same peptide molecule ("reiterative motifs"), marked enhancement of the binding capacity occurs. Isotype specificity (IAd vs IEd binding capacities) is retained in all three antigenic determinants so far analyzed (lambda rep 12-26, OVA 323-339, and hen egg lysozyme 105-120). The mechanism involved in such an effect is not clear, but experiments involving introduction of a peptide spacer between two repeated core regions do not support the notion that the effect is mediated by cross-linking of more than one MHC molecule, favoring the possibility that conformational effects or distinct subsites of interaction on the MHC molecule may be involved. Based on reiterative structures, a peptide molecule composed of only two different amino acids (Ala and His) has been produced that still retains a very high binding affinity. An 125I-radiolabeled form of this peptide has been used to demonstrate that the high binding detected is mediated by the same binding site involved in the interaction of IAd and OVA 323-339. Inhibition of Ag presentation studies further supports the immunologic relevance of the phenomena observed. Finally, we observed naturally occurring clustered binding sites in proximity of immunodominant protein regions, raising the possibility that the phenomenon might have a physiologic counterpart.
Integrating content-based access mechanisms with hierarchical file systems (1999)

We describe a new file system that provides, at the same time, both name and content based access to files. To make this possible, we introduce the concept of a semantic directory. Every semantic directory has a query associated with it. When a user creates a semantic directory, the file system automatically creates a set of pointers to the files in the file system that satisfy the query associated with the directory. This set of pointers is called the query-result of the directory. To access the files that satisfy the query, users just need to de-reference the appropriate pointers. Users can also create files and sub-directories within semantic directories in the usual way. Hence, users can organize files in a hierarchy and access them by specifying path names, and at the same time, retrieve files by asking queries that describe their content. Our file system also provides facilities for query-refinement and customization. When a user creates a new semantic sub-directory within a semantic directory, the file system ensures that the query-result of the sub-directory is a subset of the query-result of its parent. Hence, users can create a hierarchy of semantic directories to refine their queries. Users can also edit the set of pointers in a semantic directory, and thereby modify its query-result without modifying its query or the files in the file system. In this way, users can customize the results of queries according to their personal tastes, and use customized results to refine queries in the future. That is, users do not have to depend solely on the query language to achieve these objectives. Our file system has many other features, including semantic mount-points that allow users to access information in other file systems by content. The file system does not depend on the query language used for content-based access. Hence, it is possible to integrate any content-based access mechanism into our file system.
Universal bound on the performance of lattice codes (1999)

We present a lower bound on the probability of symbol error for maximum-likelihood decoding of lattices and lattice codes on a Gaussian channel. The bound is tight for error probabilities and signal-to-noise ratios of practical interest, as opposed to most existing bounds that become tight asymptotically for high signal-to-noise ratios. The bound is also universal; it provides a limit on the highest possible coding gain that may be achieved, at specific symbol error probabilities, using any lattice or lattice code in n dimensions. In particular, it is shown that the effective coding gains of the densest known lattices are much lower than their nominal coding gains. The asymptotic (as n/spl rarr//spl infin/) behavior of the new bound is shown to coincide with the Shannon (1948) limit for Gaussian channels.
The Impact of the Polymerase Chain Reaction in Clinical Medicine (2000)

netic information (elucidated by PCR) already forms the basis of some clinical testing and will do so increasingly in the future. Thus, physicians need to understand how PCR-based diagnostics are used to detect the presence of infectious disease agents and mutations associated with simple genetic diseases. Moreover, many diseases not traditionally thought of as genetic are now being evaluated in terms of inherited susceptibilities, or as genetically mediated maladaptive responses to external stimuli. In the future, molecular diagnostics that evaluate constellations of complex genetic factors may be used to assess medical risks, based on patients’ occupation, lifestyle, and family history. An enzymatic in vitro DNA amplification method, 1,2 PCR can be coupled with a number of detection methods to identify any gene (by DNA) or its expression (by messenger RNA [mRNA]) directly from clinical samples, no mat
Adenosine and Adenosine Receptors (1990)

Historical Perspective. Adenosine Receptors: An Historical Perspective. Characterization of the Receptor and Its Binding Site. Radioligand Binding Assays for Adenosine Receptors. Structure-Activity Relationships of Adenosine A1 and A2 Receptors. Biochemical Mechanisms of Receptor Action. Signal Transduction Mechanisms for Adenosine. Electrophysiological Aspects of Adenosine Receptor Function. Adenosine Release. Adenosine Deaminase and [3H]Nitrobenzylthioinisine as Markers of Adenosine Metabolism and Transport in Central Purinergic Systems. Physiological Functions of Adenosine Receptors. Adenosine and Cardiovascular Function. Adenosine and Renal Function. The Role of Adenosine in Respiratory Physiology. Adenosine Central Nervous System Function. Receptor Regulation. Adenosine and Host Defense: Modulation through Metabolism and Receptor-Mediated Mechanisms. Future Vistas. Adenosine Receptors: Future Vistas. Index.
Cortical mechanisms of loss of consciousness: insight from TMS/EEG studies. (2013)

In a recent series of experiments we recorded the electroencephalogram (EEG) response to a direct cortical stimulation in humans during wakefulness, NREM sleep, REM sleep and anesthesia by means of a combination of transcranial magnetic stimulation (TMS) and high-density EEG (hd-EEG). TMS/hd-EEG measurements showed that, while during wakefulness and REM sleep the brain is able to sustain long-range specific patterns of activation, during NREM sleep and Midazolam-induced anesthesia, when consciousness fades, this ability is lot: the thalamocortical system, despite being active and reactive, either breaks down in causally independent modules (producing a local slow wave), or it bursts into an explosive and non-specific response (producing a global EEG slow wave). We hypothesize that, like spontaneous sleep slow waves, the slow waves triggered by TMS during sleep and anaesthesia are due to bistability between upand down-states in thalamocortical circuits. In this condition, the inescapable occurrence of a silent, down state after an initial activation impairs the ability of thalamocortical circuits to sustain long-range, differentiated patterns of activation, a theoretical requisite for consciousness. According to animal experiments and computer simulations, thalamocortical bistability may result from increased K-currents, from alterations of the balance between excitation and inhibition and from partial cortical de-afferentation. We hypothesize that these factor may play an important role in determining loss, and recovery, of consciousness also in brain-injured subjects. If this is the case, some types of brain lesions may impair information transmission, above and beyond the associated anatomical disconnection, by inducing bistability in portions of the thalamocortical system that are otherwise healthy.
Revisiting read wear (2009)

In this paper, we show that people frequently return to previously-visited regions within their documents, and that scrollbars can be enhanced to ease this task. We analysed 120 days of activity logs from Microsoft Word and Adobe Reader. Our analysis shows that region revisitation is a common activity that can be supported with relatively short recency lists. This establishes an empirical foundation for the design of an enhanced scrollbar containing scrollbar marks that helps people return to previously visited document regions. Two controlled experiments show that scrollbar marks decrease revisitation time, and that a large number of marks can be used effectively. We then design an enhanced Footprints scrollbar that supports revisitation with several features, including scrollbar marks and mark thumbnails. Two further experiments show that the Footprints scrollbar was frequently used and strongly preferred over traditional scrollbars.
Improved lasing performance of XeCl using Ar and Ne diluents (1979)

The lasing-energy equivalent to KrF is extracted from XeCl in an electric avalanche discharge using argon or neon as the diluent gas. Parametric and electrical characteristics are interpreted in terms of changing electron-energy distributions as functions of changing E/N in the discharge volume.
Cellular Interconnection Arrays (1968)

Abstract—A class of networks is described that has the capability of permuting in an arbitrary manner a set of n digital input lines onto a set of n digital output lines. The circuitry of the networks is arranged in cellular form, i. e., in a two-dimensional iterative pattern with mainly local intercell connections, where the basic cell behaves as a reversing switch with a single memory flip-flop. Various network forms are described, differing in the number of cells needed, in the shape of the array, and in the length and regularity of intercell connections. Also discussed are some ways of setting up the array to achieve a desired permutation.
[Role of lipids in the organization of the vicinity of photosystem 1 centers]. (1978)

The effect of galactolipase and phospholipase A on the spectral properties of light chloroplast fragments enriched by photosystem I has been studied. Treatment by various lipases or by two enzymes in differing sequences affected absorption and fluorescence spectra of light fragments. The concentration of phospholipids and their composition were determined before and after treatment with various enzymes. Data obtained allowed to make suggestions concerning the location and functions of various lipids.
Recombinant human tumor necrosis factor administered as a five-day continuous infusion in cancer patients: phase I toxicity and effects on lipid metabolism. (1988)

Recombinant human tumor necrosis factor (rH-TNF) is a cytotoxic monokine with pleiotropic effects. A phase I trial of rH-TNF was initiated using a five-day continuous intravenous (IV) infusion repeated every 28 days. Thirty-eight courses of therapy were administered to 19 patients. The starting dose was 5 X 10(4) U/m2/d, with escalations to 1.0 X 10(5), 2.0 X 10(5), 2.4 X 10(5), and 3.0 X 10(5) U/m2/d. Systemic side effects, including fever, chills, hypotension, fatigue, anorexia, and headaches, were mild and self-limiting. At the maximum tolerated dose of 3.0 X 10(5) U/m2/d, dose-limiting hematologic toxicity was manifested by transient thrombocytopenia and leukopenia. Elevated bilirubin levels were also seen at the higher dose levels. Lipoprotein analysis demonstrated that the five-day treatment with rH-TNF was associated with decreases in high-density lipoproteins, as well as increases in triglycerides and very-low-density lipoproteins. Pharmacokinetic studies using an enzyme-linked immunosorbent assay (ELISA) test indicated plasma rH-TNF levels less than 0.2 U/mL. The recommended phase II dose of rH-TNF administered as a five-day continuous infusion is 2.4 X 10(5) U/m2/d.
Three-dimensional finite element models from magnetic resonance images as a structural framework for continuum analysis of the heart (1995)

Magnetic resonance imaging (MRI) is an extremely versatile technique for noninvasive imaging of the anatomy, structure, and physiological function of the heart and other soft tissues and organs. Although mathematical models have often been used to enhance the information content of medical images, these models are most often based on the physics of the imaging system rather that the properties of the target organ or tissue. We use finite element (FE) models of regional mechanical and electrical function in the intact heart to compute 3D distributions of important physiological field variables, such as myocardial stress, that cannot be imaged directly. A parametric model of the heart based on the physical properties of the organ as a material continuum provides a general and convenient way to synthesize clinical data, such as multidimensional images, with experimental tests, such as biomechanical and histological studies.
One-Time Password (OTP) Pre-Authentication (2012)

The Kerberos protocol provides a framework authenticating a client
using the exchange of pre-authentication data. This document describes
the use of this framework to carry out One-Time Password (OTP)
authentication. [STANDARDS-TRACK]
Structural Basis for a Bispecific NADP+ and CoA Binding Site in an Archaeal Malonyl-Coenzyme A Reductase (2013)

Autotrophic members of the Sulfolobales (crenarchaeota) use the 3-hydroxypropionate/4-hydroxybutyrate cycle to assimilate CO2 into cell material. The product of the initial acetyl-CoA carboxylation with CO2, malonyl-CoA, is further reduced to malonic semialdehyde by an NADPH-dependent malonyl-CoA reductase (MCR); the enzyme also catalyzes the reduction of succinyl-CoA to succinic semialdehyde onwards in the cycle. Here, we present the crystal structure of Sulfolobus tokodaii malonyl-CoA reductase in the substrate-free state and in complex with NADP(+) and CoA. Structural analysis revealed an unexpected reaction cycle in which NADP(+) and CoA successively occupy identical binding sites. Both coenzymes are pressed into an S-shaped, nearly superimposable structure imposed by a fixed and preformed binding site. The template-governed cofactor shaping implicates the same binding site for the 3'- and 2'-ribose phosphate group of CoA and NADP(+), respectively, but a different one for the common ADP part: the β-phosphate of CoA aligns with the α-phosphate of NADP(+). Evolution from an NADP(+) to a bispecific NADP(+) and CoA binding site involves many amino acid exchanges within a complex process by which constraints of the CoA structure also influence NADP(+) binding. Based on the paralogous aspartate-β-semialdehyde dehydrogenase structurally characterized with a covalent Cys-aspartyl adduct, a malonyl/succinyl group can be reliably modeled into MCR and discussed regarding its binding mode, the malonyl/succinyl specificity, and the catalyzed reaction. The modified polypeptide surrounding around the absent ammonium group in malonate/succinate compared with aspartate provides the structural basis for engineering a methylmalonyl-CoA reductase applied for biotechnical polyester building block synthesis.