gebhart/patrepeval huggingface dataset:
Inhibitory Effect of Glycyrrhizin on the Phosphorylation and DNA-Binding Abilities of High Mobility Group Proteins 1 and 2 in Vitro. (2001)

The physiological correlation between glycyrrhizin (GL) and high mobility group proteins I and 2 (HMG1/2) and the inhibitory effect of GL on their phosphorylation by three protein kinases (CK-I, CK-II and PKC) were investigated biochemically in vitro. It was found that GL binds directly to HMG1/2, because (i) HMG1/2 have a high affinity with a GL-affinity column; and (ii) GL induces the conformational changes in HMG1/2. Both purified HMG1/2 functioned as phosphate acceptors for these two protein kinases (CK-I and PKC), but not phosphorylated by CK-II. Phosphorylation of HMG1/2 by two protein kinases (CK-I and PKC) was completely inhibited by a glycyrrhetinic acid derivative (oGA) at one-tenth the concentration of GL. Also, the DNA-binding abilities of HNG1/2 were reduced by GL in a dose-dependent manner. These results show that the binding of GL to HMG1/2 results in the inhibition of their physiological activities (DNA-binding ability and phosphorylation by PKC or CK-I) in vitro. The GL-induced inhibition of the physiological activities of HMG1/2 may be involved in the anti-inflammatory effect of GL in vivo.
Diploid predominance in hereditary nonpolyposis colorectal carcinoma evaluated by flow cytometry (1990)

Fifty-nine colorectal carcinomas of patients with verified cancer family syndrome (CFS) were analyzed for DNA ploidy using flow cytometry. Sixty-eight percent of the tumors were diploid, and 32% were aneuploid. The aneuploid tumors had a median DNA index of 1.24 (range, 1.12-1.97). In 90% of all tumors the DNA index was less than 1.27. This predominance of diploid/near-diploid tumors was seen both in primary and in metachronous carcinomas. In 21 cases a cell cycle analysis was possible. Tumors with the S-phase fraction (SPF) greater than or equal to 9.8% had a worse prognosis than tumors with the SPF of less than 9.8%. These findings suggest that the predominance of diploid/near diploid DNA values is one of the characteristics of colorectal carcinomas in CFS. This might signify the existence of two or more pathogenetically different subgroups of colorectal carcinoma and explain the proposed better prognosis of colorectal carcinoma in CFS compared with other colorectal carcinomas.
LPCVD polysilicon films with controlled curvature for optical MEMS: the MultiPoly/sup TM/ process (2002)

A new technique is presented for producing polysilicon films with predetermined stress profiles. Polysilicon films deposited by LPCVD exhibit tensile or compressive residual stresses, depending on the deposition temperature. Polysilicon films comprised of alternating tensile and compressive layers can display any overall value of stress and stress gradient. We call the multilayer material MultiPoly/sup TM/ and the process the MultiPoly/sup TM/ process. Structures made from these films can be designed to display deliberate curvatures as-fabricated resulting in novel optical devices, such as focusing mirrors. Electrostatic actuation can be used to modify the shapes of these polysilicon structures, creating optical switches.
In VitroAntiretroviral Properties of S/GSK1349572, a Next-Generation HIV Integrase Inhibitor (2010)

S/GSK1349572 is a next-generation HIV integrase (IN) inhibitor designed to deliver potent antiviral activity with a low-milligram once-daily dose requiring no pharmacokinetic (PK) booster. In addition, S/GSK1349572 demonstrates activity against clinically relevant IN mutant viruses and has potential for a high genetic barrier to resistance. S/GSK1349572 is a two-metal-binding HIV integrase strand transfer inhibitor whose mechanism of action was established through in vitro integrase enzyme assays, resistance passage experiments, activity against viral strains resistant to other classes of anti-HIV agents, and mechanistic cellular assays. In a variety of cellular antiviral assays, S/GSK1349572 inhibited HIV replication with low-nanomolar or subnanomolar potency and with a selectivity index of 9,400. The protein-adjusted half-maximal effective concentration (PA-EC(50)) extrapolated to 100% human serum was 38 nM. When virus was passaged in the presence of S/GSK1349572, highly resistant mutants were not selected, but mutations that effected a low fold change (FC) in the EC(50) (up to 4.1 fold) were identified in the vicinity of the integrase active site. S/GSK1349572 demonstrated activity against site-directed molecular clones containing the raltegravir-resistant signature mutations Y143R, Q148K, N155H, and G140S/Q148H (FCs, 1.4, 1.1, 1.2, and 2.6, respectively), while these mutants led to a high FC in the EC(50) of raltegravir (11- to >130-fold). Either additive or synergistic effects were observed when S/GSK1349572 was tested in combination with representative approved antiretroviral agents; no antagonistic effects were seen. These findings demonstrate that S/GSK1349572 would be classified as a next-generation drug in the integrase inhibitor class, with a resistance profile markedly different from that of first-generation integrase inhibitors.
Metronidazole (“Flagyl”). A radiosensitizer of hypoxic cells (1974)

The radiosensitizing properties of metronidazole have been studied using bacteria, bean roots, and mammalian cells in vitro as test systems. Metronidazole has been shown to sensitize only anoxic cells in a concentration-dependent manner, a maximum enhancement ratio of 1·9 being obtained at 8 mmol/1. metronidazole concentration for mammalian cells. The effect of added SH compounds on the sensitizing effect of metronidazole was studied in bacteria and bean roots. Metronidazole sensitized bacteria to the effects of neutron irradiation by a factor of only 1·4. The mechanism of sensitization by metronidazole is discussed in the light of pulse radiolysis and ESR data, and its action compared with that of oxygen. Pharmacological data presented suggest that in vivo testing of metronidazole should be possible and that any radiosensitizing effects shown may be related to measured serum concentrations.
Nitrite and Nitric Oxide as Potential Diagnostic Markers in Acute Vascular Diseases (2011)

Measurement of nitric oxide (NO) or a suitable index marker such as nitrite could potentially be used in the diagnosis of various vascular diseases. Among its many functions, nitric oxide plays important roles in ischemia/ reperfusion injury. Nitrite has recently been found to generate nitric oxide under conditions of ischemia or hypoxia. Research into the biology of the nitrite anion may impact a wide range of vascular diseases, such as stroke, acute myocardial infarction, cardiac arrest, sepsis, or other diseases. As nitrite begins to be evaluated for its therapeutic impact on these diseases in clinical trials, it may be important to obtain frequent or continuous measurements of NO or nitrite levels in real time in order to optimize such therapies. In addition, if nitrite therapy becomes successful in these trials, it may become necessary to provide clinicians with tools to monitor the effect of such therapy in real time.
Practical UNIX and Internet Security (1996)

When Practical Unix Security was first published more than a decade ago, it became an instant classic. Crammed with information about host security, it saved many a Unix system administrator from disaster. The second edition added much-needed Internet security coverage and doubled the size of the original volume. The third edition is a comprehensive update of this very popular book - a companion for the Unix/Linux system administrator who needs to secure his or her organization's system, networks, and web presence in an increasingly hostile world. Focusing on the four most popular Unix varia
Solution and Biologically Relevant Conformations of Enantiomeric 11-cis-Locked Cyclopropyl Retinals (2002)

To gain information on the conformation of the 11-cis-retinylidene chromophore bound to bovine opsin, the enantiomeric pair (2a and 2b) of 11-cis-locked bicyclo[5.1.0]octyl retinal (retCPr) 2 was prepared and its conformation was investigated by NMR, geometry optimization, and CD calculations. This compound is also of interest since it contains a unique moiety in which a chiral cyclopropyl group is flanked by triene and enal chromophores, and hence would clarify the little-known chiroptical contribution of a cyclopropyl ring linked to polyene systems. NMR revealed that the seven-membered ring of retCPr adopts a twist chair conformation. The NMR-derived structure constraints were then used for optimizing the geometry of 2 with molecular mechanics and ab initio methods. This revealed that enantiomer 2a with a 11 beta,12 beta-cyclopropyl group exists as two populations of diastereomers depending on the twist around the 6-s bond; however, the sense of twist around the 12-s is positive in both rotamers. The theoretical Boltzmann-weighted CD obtained with the pi-SCF-CI-DV MO method and experimental spectra were consistent, thus suggesting that the conjugative effect of the cyclopropyl moiety is minimal. It was found that only the beta-cyclopropyl enantiomer 2a, but not the alpha-enantiomer 2b, binds to opsin. This observation, together with earlier retinal analogues incorporation results, led to the conclusion that the chromophore sinks into the N-terminal of the opsin receptor from the side of the 4-methylene and 15-aldehyde, and that the binding cleft accommodates 11-cis-retinal with a slightly positive twist around C12/C13. A reinterpretation of the previously published negative CD couplet of 11,12-dihydrorhodopsin also leads to a chromophoric C12/C13 twist conformation with the 13-Me in front as in 1b. Such a conformation for the chromophore accounts for both the observed biostereoselectivity of retCPr 2a and the observed negative couplet of 11,12-dihydro-Rh7.
Embryo-Derived Stem Cells: Of Mice and Men (2001)

Mouse embryonic stem cells are continuous cell lines derived directly from the fetal founder tissue of the preimplantation embryo. They can be expanded in culture while retaining the functional attributes of pluripotent early embryo cells. In particular, they can participate fully in fetal development when reintroduced into the embryo. The capacity for multilineage differentiation is reproduced in culture where embryonic stem cells can produce a wide range of well-defined cell types. This has stimulated interest in the isolation of analogous cells of human origin. Such human pluripotent stem cells could constitute a renewable source of more differentiated cells that could be employed to replace diseased or damaged tissue by cellular transplantation. In this review, the relationships between mouse embryonic stem cells, resident pluripotent cells in the embryo, and human embryo-derived cell lines are evaluated, and the prospects and challenges of embryo stem cell research are considered.
Analgesic effects of Neurotropin in mice, and comparison between analgesic effects of some drugs in SART-stress mice and normal mice (1976)

Analgesic effects of a neuroleptica "Neurotropin" (NSP) were studied in mice while the analgesic effects of other agents were studied in SART-stress mice (specific stress caused by alterating rhythm in temperature) and the results compared with those in normal mice. NSP is an extract containing many conjugated polysaccarides isolated from the skin or tissues of rabbits which had been inoculated with the living cowpox virus. (a) NSP given alone to normal mice resulted in slight analgesic effects as observed with the application of the acetic acid-, phenylquinon-writhing method or the modified Randall-Selitto method. Little effect was seen when the D'Amour-Smith was used. (b) Synergism was evident when NSP and aminopyrine or NSP and morphine were given concomitantly and the acetic acid- or phenylquinon-writhing methods were applied. (c) Analgesic effects of morphine, levomepromazine, imidazole acetic acid and particularly NSP were greater in SART-stress mice than in normal mice. With the D'Amour-Smith method, only NSP had a greater effect in SART-stress mice than in normal mice.
Blood amino acid compartmentation in mice bearing Lewis lung carcinoma. (1987)

Blood amino acid compartmentation between plasma and red blood cells was studied in mice bearing the Lewis lung carcinoma. The animals showed a change in compartmentation with an increase in the concentration of most amino acids in the plasmatic fraction with the exception of glycine, glutamine, aspartate, asparagine, and taurine. This work focuses on the importance of studying the distribution of amino acids in both fractions when performing studies concerning amino acid metabolism in tumor-bearing animals.
The structure of C-polysaccharide from the walls of Streptococcus pneumoniae (1978)

The well-known immologically active component of pneumococci, C-polysaccharide, is a teichoic acid that can be isolated from the cell walls and purified by Sephadex and ion-exchange chromatography. Further details of the structure of C-teichoic acid were established by chemical degradation, including hydrolysis in acid and alkali, treatment with HF, periodate oxidation and methylation. In addition, the use of 13C n.m.r. has confirmed some of these structural features and resulted in a proposal for the order of substituents, the location of positions of substitution and the configuration of anomeric centres in the repeating unit of the polymer.
VRAIS Panel on Networked Virtual Environments (1995)

Proceedings of the 1995 IEEE Virtual Reality Annual Symposium, 11 - 15 March 1995, RTP, North Carolina, pp. 230-231.
ICGI：过去、现在及未来 (2002)

Cotton is viewed as the most important cash crop in the world, and sustains the agricultural economies of many nations by providing a sustainable fiber product for the textilindustries.Unfortunately, many challenges face cotton production at present and in the future. ……
Evaluation of genetically engineered herpes simplex viruses as oncolytic agents for human malignant brain tumors. (1997)

Earlier studies have shown that genetically engineered herpes simplex viruses (e.g., HSV-1) are effective in killing malignant tumor cells both in vitro and in various murine tumor models. This report focuses on a panel of five genetically engineered viral mutants of the gamma(1)34.5 gene, which was shown previously to cause reduction in viral replication and associated neurovirulence of HSV. These include R3616, which has both copies of gamma(1)34.5 deleted, R4009, which has a stop codon inserted after codon 28 in both copies of the gamma(1)34.5 gene, R849, which contains a lacZ gene inserted in place of the gamma(1)34.5, R908, which lacks 41 codons in frame after codon 72 of the gamma(1)34.5, and R939, which carries a stop codon precluding the translation of the COOH-terminal domain of the gamma(1)34.5 gene. We report the following: (a) all five mutant HSVs were avirulent in experimental animals but were cytotoxic for human tumor cells in vitro and in vivo; (b) the gamma(1)34.5- HSV replicated in human glioma cells almost as efficiently as wild-type HSV-1(F) based on replication assays, in situ hybridization for viral DNA, and expression of infected cell protein 27; (c) capacity of mutant HSVs to kill human cells derived from glioblastoma multiforme (CH-235MG, D-37MG, D-54MG, D-65MG, U-251MG, U-373MG, and SK-MG-1), anaplastic astrocytoma (Hs-683), anaplastic glioma (U-87MG and U-138MG), gliosarcoma (D-32GS), or normal human astrocytes demonstrated that glioma cells varied in their susceptibility to HSV-mediated cytotoxicity and that cultured astrocytes were two to three orders of magnitude less susceptible to killing than were malignant glia; and (d) scid mice, which received 0.5 or 5 x 10(6) plaque-forming units of R4009, either were coinoculated at the time of intracranial transplantation with 106 U251MG or D-54MG human glioma cells or received the cells intratumorally 5 days after tumor induction and experienced significant increases in median survivals, with no histopathological indication of an infectious encephalitic process. Genetically engineered gamma(1)34.5- HSV mutants appear to be a potentially safe biotherapeutic agent for experimental treatment of uniformly fatal malignant brain tumors.
A K+ transport ATPase in Escherichia coli. (1978)

A K+ -stimulated ATPase in membranes of Escherichia coli has been identified as an activity of the Kdp system, and ATP-driven K+ transport system. Three characteristics support association of the ATPase with the Kdp system: (i) ATPase and Kdp transport are both repressed by growth in media containing high concentrations of K+; (ii) the ATPase and Kdp system accept only K+ as substrate, neither requires Na+ nor accepts Rb+ as a substrate; (iii) the affinity of the ATPase and that of th Kdp system for K+ is similar and is altered by mutations in the structural genes of the Kdp system. Discovery of an ATPase associated with a bacterial transport system suggests functional similarities with the ATP-driven transport systems of animal cells.
Mechanism of lymphoma cell death induced by cyclic AMP. (1975)

A mouse lymphoma tissue culture line, S49, is killed by isoproterenol, choleratoxin, or prostaglandin E1, inducers of cyclic AMP (cAMP) in these cells, or by the analog dibutyryl (db) cAMP. Cell death follows arrest in the G1 phase of the cell cycle. Mutant subclones obtained by growing S49 with dbcAMP were resistant to killing. They were deficient in cAMP-dependent protein kinase. These results are discussed in relation to the possible physiologic role of cAMP-induced cell death in T-cell differentiation.
Alternative molecular formats and therapeutic applications for bispecific antibodies (2015)

Bispecific antibodies are on the cusp of coming of age as therapeutics more than half a century after they were first described. Two bispecific antibodies, catumaxomab (Removab®, anti-EpCAM × anti-CD3) and blinatumomab (Blincyto®, anti-CD19 × anti-CD3) are approved for therapy, and >30 additional bispecific antibodies are currently in clinical development. Many of these investigational bispecific antibody drugs are designed to retarget T cells to kill tumor cells, whereas most others are intended to interact with two different disease mediators such as cell surface receptors, soluble ligands and other proteins. The modular architecture of antibodies has been exploited to create more than 60 different bispecific antibody formats. These formats vary in many ways including their molecular weight, number of antigen-binding sites, spatial relationship between different binding sites, valency for each antigen, ability to support secondary immune functions and pharmacokinetic half-life. These diverse formats provide great opportunity to tailor the design of bispecific antibodies to match the proposed mechanisms of action and the intended clinical application.
Superlens breaks optical barrier (2005)

One of the best known properties of light is that it diffracts, bending or spreading around objects that lie in its path. A familiar example is when a collimated beam of light passes through a small aperture in an opaque barrier. If the aperture is large, the light emerges as a beam with the same radius as that of the aperture. But if the size of the aperture is similar to the wavelength of the incident light, the emerging light flares out from the aperture and forms a diffraction pattern whereby the intensity of the transmitted light has a broad central peak.
Putting data on a diet (1993)

The use of data compression to reduce bandwidth and reduce storage requirements is discussed. The merits of lossless versus lossy compression techniques, the latter offering far greater compression ratios, are considered. The limits of lossless compression are discussed, and a simple method for lossless compression, runlength encoding, is described, as are the more sophisticated Huffman codes, arithmetic coding, and the trie-based codes invented by A. Lempel and J. Ziv (1977, 1978), WAN applications as well as throughput and latency are briefly considered.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
QuantiSNP: an Objective Bayes Hidden-Markov Model to detect and accurately map copy number variation using SNP genotyping data (2007)

Array-based technologies have been used to detect chromosomal copy number changes (aneuploidies) in the human genome. Recent studies identified numerous copy number variants (CNV) and some are common polymorphisms that may contribute to disease susceptibility. We developed, and experimentally validated, a novel computational framework (QuantiSNP) for detecting regions of copy number variation from BeadArray SNP genotyping data using an Objective Bayes Hidden-Markov Model (OB-HMM). Objective Bayes measures are used to set certain hyperparameters in the priors using a novel re-sampling framework to calibrate the model to a fixed Type I (false positive) error rate. Other parameters are set via maximum marginal likelihood to prior training data of known structure. QuantiSNP provides probabilistic quantification of state classifications and significantly improves the accuracy of segmental aneuploidy identification and mapping, relative to existing analytical tools (Beadstudio, Illumina), as demonstrated by validation of breakpoint boundaries. QuantiSNP identified both novel and validated CNVs. QuantiSNP was developed using BeadArray SNP data but it can be adapted to other platforms and we believe that the OB-HMM framework has widespread applicability in genomic research. In conclusion, QuantiSNP is a novel algorithm for high-resolution CNV/aneuploidy detection with application to clinical genetics, cancer and disease association studies.
Scheduling I/O in virtual machine monitors (2008)

This paper explores the relationship between domain scheduling in avirtual machine monitor (VMM) and I/O performance. Traditionally, VMM schedulers have focused on fairly sharing the processor resources among domains while leaving the scheduling of I/O resources as asecondary concern. However, this can resultin poor and/or unpredictable application performance, making virtualization less desirable for applications that require efficient and consistent I/O behavior.
Syntheses of 9‐(2′‐monoethylphosphonomethoxyethyl)‐8‐[14C]guanine ([14C]‐EPMG) and 9‐(2′‐phosphonomethoxyethyl)‐8‐[14C]guanine ([14C]‐PMEG) (1993)

Abstract The synthesis of the title compounds [ 14 C]‐PMEG ( 5 ) and [ 14 c]‐EPMG ( 6 ) are described. Treatment of [ 14 C]guanine with acetic anhydride in 1‐methyl‐2‐pyrrolidinone gave [ 14 C]N‐acetylguanine ( 2 ). Reaction with 2‐(diethylphosphonomethoxy) ethylmethanesulfonate in N,N‐dimethylformamide in the presence of potassium carbonate yielded 2‐N‐acetyl‐9(2′‐diethylphosphonomethoxyethyl)‐8‐[ 14 C]‐guanine ( 3 ). Removal of the acetyl group with aqueous methylamine gave 9‐(2′‐ (diethylphosphonomethoxyethyl)‐8‐[ 14 C]‐guanine ( 4 ). Treatment of the diethylphosphonate ester with sodium hydroxide and acidification with hydrochloric acid gave 9‐(2′‐(monoethylphosphonomethoxyethyl)‐ 8‐[ 14 C]‐guanine ( 5 ). Silation of the diethylphosphonate ester with bromotrimethylsilane and treatment with water produced 9‐(2′‐phosphonomethyloxethyl)‐8‐[ 14 C]guanine ( 6 ).