gebhart/patrepeval huggingface dataset:
The Sialic Acids (1971)

The Svennerholm resorcinol method for the quantitative determination of the sialic acids was modified by introducing a periodate oxidation step prior to heating with the resorcinol reagent. Sialic acid glycosides could be determined without acid hydrolysis. The glycosides gave chromogens stable to periodate oxidation at 37°, whereas free sialic acid gave chromogens that were destroyed at this temperature, but were stable at 0°. Using these properties, the periodate-resorcinol method was applied to the determination of total, free, or bound sialic acid. The periodate-resorcinol method was substantially more sensitive than the resorcinol procedure, was not affected by lipids, amino acids, or sugars, and could be used to detect free or glycosidically bound sialic acids on paper chromatograms.
Thrombosis complicating advanced non-small cell lung cancer (NSCLC) treatment (2004)

8082 Background: Lung cancer is the leading cause of cancer death and thromboembolism (TE) is a significant cause of morbidity and mortality in this disease. The exact incidence of TE in lung cancer is unknown (Am J Hematol 72: 43–52, 2003). This study reports the incidence of TE in patients (pts) with advanced NSCLC participating in a randomized clinical trial comparing chemotherapy regimens with and without platinum. Methods: Eligibility: pathological stage IIIB/IV NSCLC, PS 0–2, and no prior chemotherapy. Randomized pts received either a platinum based (PB, carboplatin and docetaxel) or a non-platinum based (NPB, gemcitabine and docetaxel) therapy. The incidence of serious adverse events (SAEs) was collected for all pts and analyzed by treatment group. SAE criteria 1) death; 2) inpatient hospitalization, 3) prolongation of existing hospitalization; 4) significant disability/incapacity; 5) a congenital anomaly/birth defect; or 6) a life-threatening event. Results: To date, 732 pts have been enrolled: The study groups are balanced for gender, age, PS, stage, and histology. A total of 319 SAEs by pt have been reported; 135 pts on PB and 184 pts on NPB. 25% of the SAEs were treatment related and 91% were hospitalizations. The most common SAEs were pneumonia (69 pts - 9%), progressive disease (47 pts - 6%), and dehydration (27 pts - 3.7%). TE was the primary SAE in 42 pts (6%), 26 pts on the NPB arm and 16 pts on the PB arm. The Fischer's exact test for difference between treatment arms was p = 0.09. Of the TEs, 52% were pulmonary emboli, 36% deep venous thrombosis, and 12% both. Conclusions: To our knowledge, this is the first large prospective trial to evaluate the incidence of clinically significant thrombosis in advanced NSCLC. There is no statistical difference between the arms in terms of TE, although TE was more common in patients treated with NPB therapy. TEs are serious adverse events of which may potentially be prevented by prophylactic heparin. Unfractionated and low molecular weight heparin have demonstrated favorable effects on the course of NSCLC and these effects are currently under investigation in our institution. Funded in part by CA23108. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Aventis Aventis Abbott; Amgen; Aventis; Bristol-Myers Squibb; Genentech; GlaxoSmithKline; Ortho Biotech; Ligand; Merck; Pfizer; OSI; Roche
The Development of Mother-Infant Interactions after Neonatal Amygdala Lesions in Rhesus Monkeys (2004)

As part of ongoing studies on the neurobiology of socioemotional behavior in the nonhuman primate, we examined the development of mother-infant interactions in 24 macaque monkeys who received either bilateral amygdala or hippocampus ibotenic acid lesions, or a sham surgical procedure at 2 weeks of age. After surgery, the infants were returned to their mothers and reared with daily access to small social groups. Behavioral observations of the infants in dyads (mother-infant pairs alone), tetrads (two mother-infant pairs), and social groups (six mother-infant pairs and one adult male) revealed species-typical mother-infant interactions for all lesion conditions, with the exception of increased physical contact time between the amygdala-lesioned infants and their mothers. Immediately after permanent separation from their mothers at 6 months of age, the infants were tested in a mother preference test that allowed the infants to choose between their mother and another familiar adult female. Unlike control and hippocampus-lesioned infants, the amygdala-lesioned infants did not preferentially seek proximity to their mother, nor did they produce distress vocalizations. Given the normal development of mother-infant interactions observed before weaning, we attribute the behavior of the amygdala-lesioned infants during the preference test to an impaired ability to perceive potential danger (i.e., separation from their mother in a novel environment), rather than to a disruption of the mother-infant relationship. These results are consistent with the view that the amygdala is not essential for fundamental aspects of social behavior but is necessary to evaluate potentially dangerous situations and to coordinate appropriate behavioral responses.
A Heterologous DNA Prime-Venezuelan Equine Encephalitis Virus Replicon Particle Boost Dengue Vaccine Regimen Affords Complete Protection from Virus Challenge in Cynomolgus Macaques (2007)

A candidate vaccine (D1ME-VRP) expressing dengue virus type 1 premembrane and envelope proteins in a Venezuelan equine encephalitis (VEE) virus replicon particle (VRP) system was constructed and tested in conjunction with a plasmid DNA vaccine (D1ME-DNA) expressing identical dengue virus sequences. Cynomolgus macaques were vaccinated with three doses of DNA (DDD), three doses of VRP (VVV group), or a heterologous DNA prime-VRP boost regimen (DDV) using two doses of DNA vaccine and a third dose of VRP vaccine. Four weeks after the final immunization, the DDV group produced the highest dengue virus type 1-specific immunoglobulin G antibody responses and virus-neutralizing antibody titers. Moderate T-cell responses were demonstrated only in DDD- and DDV-vaccinated animals. When vaccinated animals were challenged with live virus, all vaccination regimens showed significant protection from viremia. DDV-immunized animals were completely protected from viremia (mean time of viremia = 0 days), whereas DDD- and VVV-vaccinated animals had mean times of viremia of 0.66 and 0.75 day, respectively, compared to 6.33 days for the control group of animals.
A distributed resource management architecture that supports advance reservations and co-allocation (2003)

The realization of end-to-end quality of service (QoS) guarantees in emerging network-based applications requires mechanisms that support first dynamic discovery and then advance or immediate reservation of resources that will often be heterogeneous in type and implementation and independently controlled and administered. We propose the Globus Architecture for Reservation and Allocation (GARA) to address these four issues. GARA treats both reservations and computational elements such as processes, network flows, and memory blocks as first-class entities, allowing them to be created, monitored, and managed independently and uniformly. It simplifies management of heterogeneous resource types by defining uniform mechanisms for computers, networks, disk, memory, and other resources. Layering on these standard mechanisms, GARA enables the construction of application-level co-reservation and co-allocation libraries that applications can use to dynamically assemble collections of resources, guided by both application QoS requirements and the local administration policy of individual resources. We describe a prototype GARA implementation that supports three different resource type-parallel computers, individual CPU under control of the dynamic soft real-time scheduler, and integrated services networks, and provide performance results that quantify the costs of our techniques.
CEREBRAL EFFECTS OF EXPERIMENTAL HYPOTHERMIA (1954)

SINCE hypothermia was first suggested as a technique which would permit intracardiac surgery under direct vision,* the possibility of a deleterious effect of the low body temperatures upon the brain has been considered. It has been observed that animals † and humans ‡ enter a narcotic state when the rectal temperatures fall below 30 C. Although no gross cerebral damage has been noted from simple cooling in dogs, it was considered expedient to study the cerebral effects of cooling more carefully and to search for any evidence of permanent injury to the central nervous system. Monkeys, being the highest order of animals available, were chosen for this study. Survival rate, electroencephalographic changes, behavior test performance, reflexes, and changes in cardiovascular and respiratory control were selected as factors in the assessment of cerebral function. Simpson7investigated the effects of lowering the body temperature of the monkey under ether anesthesia, with
Chemical Analysis of Single Human Erythrocytes (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleChemical Analysis of Single Human ErythrocytesEdward S. YeungCite this: Acc. Chem. Res. 1994, 27, 12, 409–414Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://pubs.acs.org/doi/10.1021/ar00048a004https://doi.org/10.1021/ar00048a004research-articleACS PublicationsRequest reuse permissionsArticle Views148Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Herpes Simplex Virus Type 2 Vaccines: New Ground for Optimism? (2004)

The development of effective prophylactic and therapeutic vaccines against genital herpes has proven problematic. Difficulties are associated with the complexity of the virus life cycle (latency) and our relatively poor understanding of the mechanism of immune control of primary and recurrent disease. The types of effector cells and the mechanisms responsible for their activation and regulation are particularly important. Studies from my and other laboratories have shown that recurrent disease is prevented by virus-specific T helper 1 (Th1) cytokines (viz., gamma interferon) and activated innate immunity. Th2 cytokines (viz., interleukin-10 [IL-10]) and regulatory (suppressor) T cells downregulate this immune profile, thereby allowing unimpeded replication of reactivated virus and recurrent disease. Accordingly, an effective therapeutic vaccine must induce Th1 immunity and be defective in Th2 cytokine production, at least IL-10. These concepts are consistent with the findings of the most recent clinical trials, which indicate that (i) a herpes simplex virus type 2 (HSV-2) glycoprotein D (gD-2) vaccine formulated with a Th1-inducing adjuvant has prophylactic activity in HSV-2- and HSV-1-seronegative females, an activity attributed to the adjuvant function, and (ii) a growth-defective HSV-2 mutant (ICP10DeltaPK), which is deleted in the Th2-polarizing gene ICP10PK, induces Th1 immunity and has therapeutic activity in both genders. The ICP10DeltaPK vaccine prevents recurrent disease in 44% of treated subjects and reduces the frequency and severity of recurrences in the subjects that are not fully protected. Additional studies to evaluate these vaccines are warranted.
Ribozyme-mediated cleavage of c-fos mRNA reduces gene expression of DNA synthesis enzymes and metallothionein. (1991)

The c-fos gene product Fos has been implicated in many cellular processes, including signal transduction, DNA synthesis, and resistance to antineoplastic agents. A fos ribozyme (catalytic RNA) was designed to evaluate the effects of suppressing Fos protein synthesis on expression of enzymes involved in DNA synthesis, DNA repair, and drug resistance. DNA encoding the fos ribozyme (fosRb) was cloned into the pMAMneo expression plasmid, and the resultant vector was transfected into A2780DDP cells resistant to the chemotherapeutic agent cisplatin. The parental drug-sensitive A2780S cells were transfected with the pMMV vector containing the c-fos gene. Morphological alterations were accompanied by significant changes in pharmacological sensitivity in both c-fos- and fosRb-transfected cells. pMAMneo fosRb transfectants revealed decreased c-fos gene expression, concomitant with reduced thymidylate (dTMP) synthase, DNA polymerase beta, topoisomerase I, and metallothionein IIA mRNAs. In contrast, c-myc expression was elevated after fos ribozyme action. Insertion of a mutant ribozyme, mainly capable of antisense activity, into A2780DDP cells resulted in smaller reductions in c-fos gene expression and in cisplatin resistance than the active ribozyme. These studies establish a role for c-fos in drug resistance and in mediating DNA synthesis and repair processes by modulating expression of genes such as dTMP synthase, DNA polymerase beta, and topoisomerase I. These studies also suggest the utility of ribozymes in the analysis of cellular gene expression.
Polycation-Induced Cell Membrane Permeability Does Not Enhance Cellular Uptake or Expression Efficiency of Delivered DNA (2010)

Polycationic materials commonly used to delivery DNA to cells are known to induce cell membrane porosity in a charge-density dependent manner. It has been suggested that these pores may provide a mode of entry of the polymer-DNA complexes (polyplexes) into cells. To examine the correlation between membrane permeability and biological activity, we used two-color flow cytometry on two mammalian cell lines to simultaneously measure gene expression of a plasmid DNA delivered with four common nonviral vectors and cellular uptake of normally excluded fluorescent dye molecules of two different sizes, 668 Da and 2 MDa. We also followed gene expression in cells sorted based on the retention of endogenous fluorescein. We have found that cell membrane porosity caused by polycationic vectors does not enhance internalization or gene expression. Based on this single-cell study, membrane permeability is found to be an unwanted side effect that limits transfection efficiency, possibly through leakage of the delivered nucleic acid through the pores prior to transcription and translation and/or activation of cell defense mechanisms that restrict transgene expression.
Mechanisms of cytotoxicity to Pim kinase inhibitor, SGI-1776, in acute myeloid leukemia (2011)

Pim kinases are Ser/Thr kinases with multiple substrates that affect survival pathways. These proteins are overexpressed in acute myeloid leukemia (AML) blasts and we hypothesized that Pim kinase inhibition would affect AML cell survival. Imidazo[1,2-b]pyridazine compound, SGI-1776 inhibits Pim-1, Pim-2 and Pim-3, and was evaluated in AML-cell line, -xenograft model, and -primary blasts. Treatment of AML cells with SGI-1776 results in a concentration-dependent induction of apoptosis and we investigated its effect on Pim kinase functions. Phosphorylation of traditional Pim kinase targets, c-Myc(Ser62) and 4E-BP1 (Thr36/Thr47), were both decreased in actively cycling AML cell lines MV-4-11, MOLM-13 and OCI-AML-3. Levels of antiapoptotic proteins Bcl-2, Bcl-x(L), XIAP, and proapoptotic Bak and Bax were unchanged; however, a significant reduction in Mcl-1 was observed. This was correlated with inhibition of global RNA and protein synthesis and MCL-1 transcript decline after SGI-1776 treatment. These data suggest that SGI-1776 mechanism in AML involves Mcl-1 protein reduction. Consistent with cell line data, xenograft model studies with mice bearing MV-4-11 tumors showed efficacy with SGI-1776. Importantly, SGI-1776 was also cytotoxic in AML primary cells, irrespective of FLT3 mutation status and resulted in Mcl-1 protein decline. Pim kinase inhibition may be a new strategy for AML treatment.
A semantic transcoding system to adapt Web services for users with disabilities (2000)

Among the most critical issues of the internet today is how to make Web content accessible to all users, especially to users with disabilities. To meet the diverse needs and abilities of this population, the Web today calls for the development of new systems and methods to enable the same content to be adapted for display according to specific, often conflicting needs. One way to achieve this goal is through Web con- tent transcoding. This paper presents a system, called Aurora, that transcodes Web content based on semantic rather than syntactic constructs. The goal is to deliver Web-based services (such as auction, search engine, travel, etc.) to a diverse set of users according to their specific needs. Using a schema-driven framework, Aurora extracts and maps Web content into domain-specific XML data based on abstract user goals. In doing so, it separates the meaning Web content from its presentation. The system further enables an extensible set of interface adaptors to generate custom Web pages, on-the-fly, from this standardized XML data. Ultimately, it streamlines and customizes the Web interface to faciliate navigation. The mechanisms of this rule-based semantic transcoding system and its advantages and limitations as a strategy to make Web services more accessible are the subject of this paper.
Convection-enhanced delivery of Ls-TPT enables an effective, continuous, low-dose chemotherapy against malignant glioma xenograft model1 (2006)

Treatment of malignant gliomas represents one of the most formidable challenges in oncology. The combination of surgery, radiation, and chemotherapy yields median survivals of less than one year. Here we demonstrate the use of a minimally invasive surgical technique, convection-enhanced delivery (CED), for local administration of a novel nanoparticle liposome containing topotecan. CED of this liposomal topotecan (Ls-TPT) resulted in extended brain tissue retention (t1/2 = 1.5 days), whereas free topotecan was rapidly cleared (t1/2 = 0.1 days) after CED. The favorable pharmacokinetic profile of extended topotecan release for about seven days, along with biodistribution featuring perivascular accumulation of the nanoparticles, provided, in addition to the known topoisomerase I inhibition, an effective antiangiogenic therapy. In the rat intracranial U87MG tumor model, vascular targeting of Ls-TPT with CED was associated with reductions in laminin expression and vascular density compared to free topotecan or control treatments. A single CED treatment on day 7 showed that free topotecan conferred no survival benefit versus control. However, Ls-TPT produced a significant (P = 0.0002) survival benefit, with six of seven complete cures. Larger U87MG tumors, where CED of Ls-TPT on day 12 resulted in one of six cures, indicated the necessity to cover the entire tumor with the infused therapeutic agent. CED of Ls-TPT was also efficacious in the intracranial U251MG tumor model (P = 0.0005 versus control). We conclude that the combination of a novel nanoparticle Ls-TPT and CED administration was very effective in treating experimental brain tumors.
Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade (2002)

PD-1 is a receptor of the Ig superfamily that negatively regulates T cell antigen receptor signaling by interacting with the specific ligands (PD-L) and is suggested to play a role in the maintenance of self-tolerance. In the present study, we examined possible roles of the PD-1/PD-L system in tumor immunity. Transgenic expression of PD-L1, one of the PD-L, in P815 tumor cells rendered them less susceptible to the specific T cell antigen receptor-mediated lysis by cytotoxic T cells in vitro, and markedly enhanced their tumorigenesis and invasiveness in vivo in the syngeneic hosts as compared with the parental tumor cells that lacked endogenous PD-L. Both effects could be reversed by anti-PD-L1 Ab. Survey of murine tumor lines revealed that all of the myeloma cell lines examined naturally expressed PD-L1. Growth of the myeloma cells in normal syngeneic mice was inhibited significantly albeit transiently by the administration of anti-PD-L1 Ab in vivo and was suppressed completely in the syngeneic PD-1-deficient mice. These results suggest that the expression of PD-L1 can serve as a potent mechanism for potentially immunogenic tumors to escape from host immune responses and that blockade of interaction between PD-1 and PD-L may provide a promising strategy for specific tumor immunotherapy.
Microglial Activation and β-Amyloid Deposit Reduction Caused by a Nitric Oxide-Releasing Nonsteroidal Anti-Inflammatory Drug in Amyloid Precursor Protein Plus Presenilin-1 Transgenic Mice (2002)

3-4-(2-Fluoro-alpha-methyl-[1,1'-biphenyl]-4-acetyloxy)-3-methoxyphenyl]-2-propenoic acid 4-nitrooxy butyl ester (NCX-2216), a nitric oxide (NO)-releasing derivative of the cyclooxygenase-1-preferring nonsteroidal anti-inflammatory drug (NSAID) flurbiprofen, dramatically reduced both beta-amyloid (Abeta) loads and Congo red staining in doubly transgenic (Tg) amyloid precursor protein plus presenilin-1 mice when administered at 375 ppm in diet between 7 and 12 months of age. This reduction was associated with a dramatic increase in the number of microglia expressing major histocompatibility complex-II antigen, a marker for microglial activation. In contrast, ibuprofen at 375 ppm in diet caused modest reductions in Abeta load but not Congo red staining, suggesting that the effects of this nonselective NSAID were restricted primarily to nonfibrillar deposits. We detected no effects of the cyclooxygenase-2-selective NSAID celecoxib at 175 ppm on amyloid deposition. In short-term studies of 12-month-old Tg mice, we found that the microglia-activating properties of NCX-2216 (7.5 mg small middle dot kg(-1) small middle dot d(-1), s.c.) were present after 2 weeks of treatment. Microglia were not activated by NCX-2216 in non-Tg mice lacking Abeta deposits, nor were microglia activated in Tg animals by flurbiprofen (5 mg small middle dot kg(-1) small middle dot d(-1)) alone. These data are consistent with the argument that activated microglia can clear Abeta deposits. We conclude that the NO-generating component of NCX-2216 confers biological actions that go beyond those of typical NSAIDs. In conclusion, NCX-2216 is more efficacious than ibuprofen or celecoxib in clearing Abeta deposits from the brains of Tg mice, implying potential benefit in the treatment of Alzheimer's dementia.
Commute path bandwidth traces from 3G networks (2013)

In this dataset paper, we present and make available real-world measurements of the throughput that was achieved at the application layer when adaptive HTTP streaming was performed over 3G networks using mobile devices. For the streaming sessions, we used popular commute routes in and around Oslo (Norway) traveling with different types of public transportation (metro, tram, train, bus and ferry). We also have a few logs using a car. Each log provides a times-tamp, GPS coordinates and the measured number of bytes downloaded for approximately every second of the route. The dataset can be used in several ways, but the most obvious application is to emulate the same network bandwidth behavior (on specific geographical positions) for repeated experiments.