gebhart/patrepeval huggingface dataset:
On-Line Fast Atom Bombardment Mass Spectrometric Detection in High-Speed Countercurrent Chromatography Through a Moving Belt Interface (1995)

A high-speed countercurrent chromatograph with on-line fast atom bombardment (FAB) mass spectrometric detection through a moving belt interface (MBI) was employed in the separation of two model systems. The relatively high flow-rate compatibility of the MBI allowed the use of a commercially-available multi-layer coil planet centrifuge with a specially designed, small-volume (16.8 mL) analytical coil. Flow rates of up to 800 μL/min were successfully deposited onto the belt using an organic-rich mobile phase. The MBI ran without generating any noticeable back-pressure on the chromatographic system and produced matrix-free FAB spectra.
The Reformatsky Reaction. I. Zinc and Ethyl α-Bromoisobutyrate* (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Reformatsky Reaction. I. Zinc and Ethyl α-Bromoisobutyrate*Wyman R. Vaughan, Stanley C. Bernstein, and Milton E. LorberCite this: J. Org. Chem. 1965, 30, 6, 1790–1795Publication Date (Print):June 1, 1965Publication History Published online1 May 2002Published inissue 1 June 1965https://pubs.acs.org/doi/10.1021/jo01017a019https://doi.org/10.1021/jo01017a019research-articleACS PublicationsRequest reuse permissionsArticle Views700Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Respiratory Syncytial Virus Interferon Antagonist NS1 Protein Suppresses and Skews the Human T Lymphocyte Response (2011)

We recently demonstrated that the respiratory syncytial virus (RSV) NS1 protein, an antagonist of host type I interferon (IFN-I) production and signaling, has a suppressive effect on the maturation of human dendritic cells (DC) that was only partly dependent on released IFN-I. Here we investigated whether NS1 affects the ability of DC to activate CD8+ and CD4+ T cells. Human DC were infected with RSV deletion mutants lacking the NS1 and/or NS2 genes and assayed for the ability to activate autologous T cells in vitro, which were analyzed by multi-color flow cytometry. Deletion of the NS1, but not NS2, protein resulted in three major effects: (i) an increased activation and proliferation of CD8+ T cells that express CD103, a tissue homing integrin that directs CD8+ T cells to mucosal epithelial cells of the respiratory tract and triggers cytolytic activity; (ii) an increased activation and proliferation of Th17 cells, which have recently been shown to have anti-viral effects and also indirectly attract neutrophils; and (iii) decreased activation of IL-4-producing CD4+ T cells--which are associated with enhanced RSV disease--and reduced proliferation of total CD4+ T cells. Except for total CD4+ T cell proliferation, none of the T cell effects appeared to be due to increased IFN-I signaling. In the infected DC, deletion of the NS1 and NS2 genes strongly up-regulated the expression of cytokines and other molecules involved in DC maturation. This was partly IFN-I-independent, and thus might account for the T cell effects. Taken together, these data demonstrate that the NS1 protein suppresses proliferation and activation of two of the protective cell populations (CD103+ CD8+ T cells and Th17 cells), and promotes proliferation and activation of Th2 cells that can enhance RSV disease.
Entonces; El visitante; Con los ojos abiertos a la orilla del mundo (2010)

This work was undertaken to measure the in situ phytoextraction of zinc using a former zinc-smelter site where metallophyte plants have been growing for 30 years. The site exhibited a gradient in the total metal concentration in the upper horizon (from 3230 to 8530 mg Zn kg(-1)). Soils were sampled from four different sectors (I-IV), and plant shoots were harvested, identified, their biomass weighed and analysed for zinc. The results showed that three plant species were dominant on the site, including Arabidopsis halleri (cress), Armeria maritima (seathrift), and Arrhenatherum elatius (fromental). A. maritima was the predominant species according to the biomass production on the most polluted sector 1. As the concentration of metals in soils decreased. A. maritima disappeared and A. halleri increased. The biomass of A. elatius was the highest on the less polluted soils. Concentrations in zinc in the aerial parts of plants varied from 73 (sector IV) to 6269 mg kg(-1) DM (sector 1). The concentration of Zn in A. halleri decreased with the decrease in concentration of zinc in soil. Phytoextraction was calculated from the biomass and its concentration of metal. It was at a maximum in sector III with a high contribution of A. halleri and A. elatius and reached 10 kg Zn ha(-1), a promising amount for phytoextraction considering the absence of any agricultural practices. In sector 1, phytoextraction was four times lower despite a 2.6 times higher concentration of Zn in the upper horizon. In conclusion, phytoextraction was strongly dependent on the concentration of the available metal in soils which may limit the growth of plants, and favour tolerant but low biomass plant species such as A. maritima.
Biosynthesis of Taxol (1997)

Taxol, a true “evergreen” in natural product chemistry! Owing to the limited supply of this successful antitumor agent in the bark of yew trees, alternative sources are being sought. Crucial information on its unusual and complex biosynthesis (shown below) has been obtained, and some enzymes responsible have been identified. The long-term goal is the efficient, biotechnology production of taxol (1) by fungi or plant tissue cultures.
Presence of serum anti-p53 antibodies is associated with pleural effusion and poor prognosis in lung cancer patients. (1998)

This study was designed prospectively to evaluate the development of anti-p53 antibodies (Abs) in lung cancer patients in relation to their clinical outcome. Sera, derived from 125 lung cancer patients, consisting of 14 small cell lung cancers (SCLC) and 111 non-SCLCs (NSCLC), were surveyed. The p53-null human NSCLC cell line, NCI-H1299, transfected with a human mutant p53 gene was prepared as the source of p53 antigen for immunoblotting analyses to detect the presence of serum anti-p53 Abs. The control group included sera from 10 healthy adults and 14 patients with benign pulmonary diseases. Clinical data including staging and survival were recorded for statistical analyses. The anti-p53 Abs were found in 8% (10 of 125) of the lung cancer patients studied (8.1% of NSCLC versus 7.1% of SCLC patients), whereas none of the control sera had detectable anti-p53 Abs. The presence of anti-p53 Abs was closely associated with malignant pleural effusions (P = 0.001). The p53 Ab-positive patients had a worse prognosis than the p53 Ab-negative patients (P < 0.02; median survival, 20 versus 41 weeks). In both univariate and multivariate analyses, the tumor extension and probably the presence of anti-p53 Abs were significant predictors for cancer death. The development of anti-p53 Abs (n = 9) was also a predictor for poor survival in patients with malignant effusions (n = 51). In conclusion, the presence of serum anti-p53 Abs is closely associated with malignant pleural effusions in lung cancer patients. It may serve as a negative prognostic factor for survival independent of malignant pleural effusions and tumor staging.
The paradox of dysfunctional high-density lipoprotein (2007)

Current data suggest that under some conditions HDL can become dysfunctional and even proinflammatory, but this characterization can change with resolution of systemic inflammation or use of certain treatments.
Heat Shock Fusion Protein gp96‐Ig Mediates Strong CD8 CTL Expansion in vivo (2002)

We conclude that secreted gp96-Ig is a powerful mediator of specific CD8+ CTL responses in vivo. Secretory gp96 mimics release of gp96 by damaged or necrotic cells that is able to activate dendritic cells without CD4 help. Gp96-Ig associated peptides have not been selected by binding to major histocompatibility complex (MHC). Specific immunization by secreted gp96-Ig therefore is expected to occur also in allogeneic settings.
Hematocrit, blood pressure, and hypertension. The Gubbio Population Study. (1992)

Baseline data from the Gubbio Population Study in north central Italy were used to investigate the relation of hematocrit to blood pressure and hypertension among 2,809 men and women aged 25-74 years. Independent of gender, age, and other confounders, the hypertensive group had a higher hematocrit than the nonhypertensive group (p less than 0.001). In comparison with the untreated hypertensive group, the hypertensive group being treated with diuretics or with other drugs only had similar mean hematocrit levels despite significantly lower blood pressures. Hematocrit was positively correlated with systolic pressure (r = 0.085, p less than 0.01 and r = 0.264, p less than 0.001 for men and women, respectively) and diastolic pressure (r = 0.214, p less than 0.001 and r = 0.266, p less than 0.001). In both sexes, whether or not the treated hypertensive group was included, age-adjusted prevalence of hypertension and average blood pressure were higher for persons in higher quintiles of hematocrit (p less than 0.001). The association of hematocrit with blood pressure and hypertension was significant and independent of several confounders. The regression coefficient of blood pressure on hematocrit ranged between 0.410 and 0.620 mm Hg per unit of hematocrit for systolic pressure and between 0.371 and 0.581 for diastolic pressure, depending on gender and whether the treated hypertensive group was included in multiple regression analysis. Based on exponentiation of the multiple logistic coefficient, prevalence of hypertension was at least two times greater for persons whose hematocrit levels were higher by 10 units.
Development of flexible microactuator and its applications to robotic mechanisms (2002)

A flexible microactuator (FMA) driven by an electropneumatic (or electrohydraulic) system has been developed. The FMA has three degrees of freedom, pitch, yaw, and stretch, and these are suitable movements for miniature robotic mechanisms such as fingers, arms, or legs. The construction is of fiber-reinforced rubber, and the mechanism is very simple. Gentle miniature robots with no conventional links can be designed using this design. The FMA's basic characteristics and its applications to certain robot mechanisms are presented. Serially connected FMAs act as a miniature robot manipulator. The kinematics and control algorithm for this type of robot are presented. FMAs combined in parallel act as a multifingered robot hand, with each FMA representing a finger. An algorithm for the cooperative control of such FMAs, the stable region for holding, and its performance are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cholesterol and Tau Protein - Findings in Alzheimer’s and Niemann Pick C’s Disease (2003)

Niemann Pick C (NPC), a fatal autosomal-recessive neurovisceral lipid storage disorder, is a juvenile dementia with massive nerve-cell loss and cytoskeletal abnormalities in cerebral neurons. These abnormalities consist of tangles of tau protein, which is otherwise highly soluble and usually stabilizes the microtubules. Immunologically and ultrastructurally similar tangles are seen some decades later in patients with Alzheimer's disease (AD). There is evidence that tangle-bearing cells in both diseases show higher levels of free (i. e. filipin-positive) cholesterol than adjacent tangle-free nerve cells. The cholesterol accumulates either in a more diffuse way (mainly in AD) or in granule-like accumulations (mainly in NPC). In NPC, neuron cholesterol may originate from sources other than the alimentary tract. Experiments with a NPC mouse model revealed that even in pure neuron cultures, the NPC -/- neurons accumulate free cholesterol in contrast to NPC-wt littermates, suggesting that the cholesterol is either synthesized by the neurons or liberated from degenerated ones before being taken up by the endosomal/lysosomal pathway. The accumulation of free cholesterol in the somata of NPC neurons is associated with a decrease of cholesterol levels in myelin sheaths. In terms of tau protein, NPC -/- mice exhibit higher levels of AT8-positive tau, suggesting that the phosphorylation-dependent mAb AT8 has detected a tau-epitope in a state considered to represent early stages of tangle formation. Concomitantly to the increase in free intracellular cholesterol, the rate-limiting enzyme in cholesterol and isoprenoid biosynthesis, HMG-CoA reductase, was found to be significantly reduced. Experimental blockade of the enzyme's activity by application of the lipid-lowering drug lovastatin showed subcellular shifts in tau phosphorylation as monitored with mAbs AT8, 12E8 and others. In summary, the data showed interesting similarities between NPC and AD suggesting some pathological metabolic pathway in common.
Optical Transceiver Modules for Gigabit Ethernet PON FTTH System (2005)

nication paths on the passive double star (PDS) transmission line with 16 branches. There is an economical advantage in sharing the optical fiber between OLT in a center office and the branching point (splitter) by two or more subscribers. Moreover, although not prescribed by the IEEE802.3ah standard, down-linking the video signal which is based on the ITU-T983.3 standard is also taken into consideration, whereby, using the 1550-nm wavelength band, video delivery through the fiber is carried out (3 wave multiplex). In a GE-PON system, the OLT performs time sharing (TDM) communication between subscribers ONUs through two or more optical fibers of different distances. Therefore, in order for the OLT to receive the up-link signal without a collision with different power levels from each ONU, the receiver of the OLT must be able to follow each power level instantaneously and to communicate in order (burst mode communication). The down stream direction is continuous mode, as in conventional communication systems. The loss budget for GE-PON systems has the specification of a 10-km and a 20-km version according to IEEE802.3ah standard, in which the fiber loss differs
The <EMPH TYPE="ITAL">APOE</EMPH>-∊4 Allele and the Risk of Alzheimer Disease Among African Americans, Whites, and Hispanics (1998)

The presence of an APOE-epsilon4 allele is a determinant of AD risk in whites, but African Americans and Hispanics have an increased frequency of AD regardless of their APOE genotype. These results suggest that other genes or risk factors may contribute to the increased risk of AD in African Americans and Hispanics.
Synthesis of Reagents for the Construction of Hypusine and Deoxyhypusine Peptides and Their Application as Peptidic Antigens (1998)

Two new synthetic methods which allow access to (2S)-deoxyhypusine, natural (2S,9R)-hypusine, (2S,9S)-hypusine, and deoxyhypusine- and hypusine-containing peptides are described. The methods involve both the construction of a deoxyhypusine reagent in which the alpha-nitrogen protecting group is orthogonal to the N-7 and N-12 protecting groups and an alternate synthesis of our previous hypusine reagent, a synthesis which provides for better stereochemical control at C-9. Synthetic hypusine and deoxyhypusine can be generated from these reagents. The hypusine-containing hexapeptide (Cys-Thr-Gly-Hpu-His-Gly) is conjugated to ovalbumin (OVA), keyhole limpet hemocyanin (KLH), and a bis-maleimide; KLH conjugates are also made with the deoxyhypusine- and lysine-containing hexapeptides. Monoclonal antibodies are generated to the hypusine-containing hexapeptide-OVA conjugate in mice. These are isolated and screened against the hypusine-containing hexapeptide-KLH and hypusine-containing hexapeptide-bis-maleimide conjugates, as well as against the deoxyhypusine-containing and lysine-containing hexapeptide-KLH conjugates. These antibodies may be useful in localizing intracellular hypusine-containing peptides as well as peptides containing hypusine analogues.
Lgr5-Positive Supporting Cells Generate New Hair Cells in the Postnatal Cochlea (2014)

The prevalence of hearing loss after damage to the mammalian cochlea has been thought to be due to a lack of spontaneous regeneration of hair cells, the primary receptor cells for sound. Here, we show that supporting cells, which surround hair cells in the normal cochlear epithelium, differentiate into new hair cells in the neonatal mouse following ototoxic damage. Using lineage tracing, we show that new hair cells, predominantly outer hair cells, arise from Lgr5-expressing inner pillar and third Deiters cells and that new hair cell generation is increased by pharmacological inhibition of Notch. These data suggest that the neonatal mammalian cochlea has some capacity for hair cell regeneration following damage alone and that Lgr5-positive cells act as hair cell progenitors in the cochlea.
Hibernoma formation in transgenic mice and isolation of a brown adipocyte cell line expressing the uncoupling protein gene. (1992)

Transgenic mice were produced containing the adipocyte-specific regulatory region from the adipocyte P2 (aP2) gene linked to the simian virus 40 transforming genes. Most of the transgenic mice developed brown fat tumors (hibernomas) in their interscapular brown adipose tissue. Hibernoma formation was noticeable in some of the mice as early as 1 day after birth and most of the mice developed very large tumors by 1 month of age. All of the tumor tissue expressed the brown fat-specific uncoupling protein (UCP) gene as well as the aP2 gene. Several of the tumors have been used to establish cultured cell lines and at least one of these lines can be induced to differentiate into brown adipocytes. The cultured adipocytes express mRNA for UCP upon stimulation with N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate, norepinephrine, isoproterenol or D7114, a beta 3 adrenergic agonist. Thus, regulation of the key thermogenic gene UCP can now be studied in an established cell line.
BackFi (2015)

We present BackFi, a novel communication system that enables high throughput, long range communication between very low power backscatter devices and WiFi APs using ambient WiFi transmissions as the excitation signal. Specifically, we show that it is possible to design devices and WiFi APs such that the WiFi AP in the process of transmitting data to normal WiFi clients can decode backscatter signals which the devices generate by modulating information on to the ambient WiFi transmission. We show via prototypes and experiments that it is possible to achieve communication rates of up to 5 Mbps at a range of 1 m and 1 Mbps at a range of 5 meters. Such performance is an order to three orders of magnitude better than the best known prior WiFi backscatter system [27,25]. BackFi design is energy efficient, as it relies on backscattering alone and needs insignificant power, hence the energy consumed per bit is small.
Rotating virtual objects with real handles (1999)

Times for virtual object rotations reported in the literature are of the order of 10 seconds or more and this is far longer than it takes to manually orient a “real” object, such as a cup. This is a report of a series of experiments designed to investigate the reasons for this difference and to help design interfaces for object manipulation. The results suggest that two major factors are important. Having the hand physically in the same location as the virtual object being manipulated is one. The other is based on whether the object is being rotatted to a new, randomly determined orientation, or is always rotated to the same position. Making the object held in the hand have the same physical shape as the object being visually manipulated was not found to be a significant factor. The results are discussed in the context of interactive virtual environments.
Amplitude and phase characterization of 45-fs pulses by frequency-resolved optical gating (1998)

The technique of second-harmonic generation frequency-resolved optical gating is applied to measure the intensity and the phase of 4.5-fs pulses resulting from the fiber-compressed output of a cavity-dumped Ti:sapphire laser. Characterization of even shorter optical pulses by this method should also be feasible.
Mutations Affecting Programmed Cell Deaths in the Nematode Caenorhabditis elegans s (1983)

Mutations in two nonessential genes specifically block the phagocytosis of cells programmed to die during development. With few exceptions, these cells still die, suggesting that, in nematodes, engulfment is not necessary for most programmed deaths. Instead, these deaths appear to occur by cell suicide.
The Anodic Evolution of Ozone (1982)

Trends of the anodic ozone evolution reaction are characterized for several different anode material and electrolyte combinations. The effect of electrolyte temperature and concentration, current density, and additions of small quantities of the fluoride ion are explored. The general behavior of ozone current efficiencies are rationalized in terms of coverage of the anode surface by adsorbed anionic material and the electronegativity of the electrolyte anions. At 0°C ozone current efficiencies of over 50% are reported for hexafluorophosphoric acid electrolyte which represent substantially higher yields than any previously achieved.
REGULATION OF GENE EXPRESSION BY HYPERTONICITY (1997)

Adaptation of cells to hypertonicity often involves changes in gene expression. Since the concentration of salt in the interstitial fluid surrounding renal inner medullary cells varies with operation of the renal concentrating mechanism and generally is very high, the adaptive mechanisms of these cells are of special interest. Renal medullary cells compensate for hypertonicity by accumulating variable amounts of compatible organic osmolytes, including sorbitol, myo-inositol, glycine betaine, and taurine. In this review we consider how these solutes help relieve the stress of hypertonicity and the nature of transporters and enzymes responsible for their variable accumulation. We emphasize recent developments concerning the molecular basis for osmotic regulation of these genes, including identification and characterization of osmotic response elements. Although osmotic stresses are much smaller in other parts of the body than in the renal medulla, similar mechanisms operate throughout, yielding important physiological and pathophysiological consequences.