gebhart/patrepeval huggingface dataset:
Differential In Vitro and In Vivo Antitumor Effects Mediated by Anti-CD40 and Anti-CD20 Monoclonal Antibodies Against Human B-Cell Lymphomas (1996)

The antitumor effects of CD40 and CD20 monoclonal antibodies (mAbs) were compared on various human B-cell lymphomas by using both in vitro and in vivo assays. Anti-CD40 directly inhibited the proliferation of human B-cell lymphomas in vitro, whereas anti-CD20 exerted no inhibitory effects on the growth of any lymphoma tested. These lymphomas were then injected into immunodeficient mice to examine the antitumor efficacy of these unconjugated mAbs in vivo. This xenogeneic model was used in the evaluation of various potential therapeutic agents against human cancers in an in vivo setting. Surprisingly, in contrast to its negligible effects on lymphoma growth in vitro, anti-CD20 was more efficacious than anti-CD40 in promoting the survival of mice bearing some but not all lymphoma lines. To determine whether the antitumor effects of these mAbs were direct or indirect in vivo, we concurrently treated tumor-bearing mice with mAbs to the murine Fc receptor to block antibody-dependent cell-mediated cytotoxicity (ADCC). When these neutralizing antibodies against Fc receptors were administered at the same time as mAb treatment, the antitumor effects of anti-CD20 in vivo were completely abrogated, whereas anti-CD40 treatment, although also diminished, still provided significant antitumor effects. These results indicate that the in vivo antitumor activity of the murine anti-human CD20 mAb was primarily due to ADCC by murine effector cells, which may not translate into comparable effects in humans. By contrast, anti-CD40 may be of potential clinical use in the treatment of lymphomas in humans because of its additional direct anti-proliferative effects. The results also demonstrate a possible difficulty in accurately evaluating the potential clinical efficacy of murine antibodies against human tumors in a human/mouse model system. Murine monoclonal anti-human antibodies may produce greater effects in human/mouse xenogeneic models, in which they are more likely to elicit host effector systems than when used in vivo in humans.
Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis. (1995)

During their development B lymphocytes pass through a maturational stage in which encounter with Ag leads to tolerance rather than activation. At least four mechanisms for achieving B cell tolerance have been reported: deletion, anergy, receptor editing, and competition for follicular niches. Although turnover rates for immature B cells in the adult mouse bone marrow and several transgenic model systems suggest that a major process contributing to negative selection of B cells is deletion, a detailed study of the negative effect of Ag-receptor engagement on primary, immature B cell survival has never been undertaken. We have utilized an in vitro culture system to determine whether cross-linking sIgM on tolerance-susceptible sIgM+IgD- B cells results in deletion by apoptosis. In contrast to the effect of sIgM cross-linking on mature splenic B cells, treatment of immature, bone marrow-derived B cells results in significant levels of apoptotic death. Ag receptor-mediated apoptosis is detectable by 14 h after sIgM engagement. Moreover, IL-4 and cycloheximide, which have previously been shown to prevent B cell tolerance induction, specifically block the sIgM-induced apoptosis observed in the immature B cells. Similarly, immature B cells from the neonatal spleen are also susceptible to apoptosis after sIgM cross-linking, although they manifest somewhat higher levels of unstimulated apoptosis as compared with bone marrow-derived B cells. These studies are the first detailed demonstration of Ag receptor-mediated apoptosis of primary immature stage B lymphocytes.
Polarisation insensitive operation of ferroelectricliquidcrystal devices (1995)

The accepted mode of operation of ferroelectric liquid crystal devices is to place them between crossed polarisers. The authors demonstrate that such devices may be used as diffractive modulators without polarisers. A one-to-many polarisation independent switch is presented for singlemode optical fibre networks, based on dynamic liquid crystal holograms.
Menopausal Hormone Replacement Therapy and Risk of Ovarian Cancer (2002)

Women who used estrogen-only replacement therapy, particularly for 10 or more years, were at significantly increased risk of ovarian cancer in this study. Women who used short-term estrogen-progestin-only replacement therapy were not at increased risk, but risk associated with short-term and longer-term estrogen-progestin replacement therapy warrants further investigation.
Practical Syntheses of Enantiomerically Enriched γ-Lactones and γ-Hydroxy Ketones by the Alkylation of Pseudoephedrine Amides with Epoxides and Their Derivatives (1996)

Pseudoephedrine amide enolates are shown to undergo efficient alkylation reactions with epoxides as electrophiles. Reactions with monosubstituted epoxides are subject to stereochemical matching such that the pairing leading to the 1,3-syn diastereomer is a highly selective, synthetically useful process, while the pairing forming the 1,3-anti diastereomer is not. Reactions with the 1,1-disubstituted epoxide isobutylene oxide are also highly diastereoselective and synthetically useful, but ethylene oxide exhibits poor diastereoselectivity. As an alternative to the use of ethylene oxide, 2-(tert-butyldimethylsilyloxy)ethyl iodide is shown to undergo highly diastereoselective and efficient alkylation reactions with pseudoephedrine amide enolates. Interestingly, epoxides and alkyl halides are found to attack opposite π-faces of pseudoephedrine amide enolates. The products of each of these alkylation reactions are transformed efficiently into γ-lactones by acidic hydrolysis and into methyl ketones by the addition of methyllithium. The methodology described provides useful procedures for the synthesis of enantiomerically enriched γ-lactones and γ-hydroxy ketones.
Propionate metabolism in the rat heart by 13C n.m.r. spectroscopy (1988)

High-resolution 13C n.m.r. spectroscopy has been used to examine propionate metabolism in the perfused rat heart. A number of tricarboxylic acid (TCA) cycle intermediates are observable by 13C n.m.r. in hearts perfused with mixtures of pyruvate and propionate. When the enriched 13C-labelled nucleus originates with pyruvate, the resonances of the intermediates appear as multiplets due to formation of multiply-enriched 13C-labelled isotopomers, whereas when the 13C-labelled nucleus originates with propionate, these same intermediates appear as singlets in the 13C spectrum since entry of propionate into the TCA cycle occurs via succinyl-CoA. An analysis of the isotopomer populations in hearts perfused with [3-13C]pyruvate plus unlabelled propionate indicates that about 27% of the total pyruvate pool available to the heart is derived directly from unlabelled propionate. This was substantiated by perfusing a heart for 2 h with [3-13C]propionate as the only available exogenous substrate. Under these conditions, all of the propionate consumed by the heart, as measured by conventional chemical analysis, ultimately entered the oxidative pathway as [2-13C] or [3-13C]pyruvate. This is consistent with entry of propionate into the TCA cycle intermediate pools as succinyl-CoA and concomitant disposal of malate to pyruvate via the malic enzyme. 13C resonances arising from enriched methylmalonate and propionylcarnitine are also detected in hearts perfused with [3-13C] or [1-13C]propionate which suggests that 13C n.m.r. may be useful as a non-invasive probe in vivo of metabolic abnormalities involving the propionate pathway, such as methylmalonic aciduria or propionic acidaemia.
Deubiquitinating and Interferon Antagonism Activities of Coronavirus Papain-Like Proteases (2010)

Coronaviruses encode multifunctional proteins that are critical for viral replication and for blocking the innate immune response to viral infection. One such multifunctional domain is the coronavirus papain-like protease (PLP), which processes the viral replicase polyprotein, has deubiquitinating (DUB) activity, and antagonizes the induction of type I interferon (IFN). Here we characterized the DUB and IFN antagonism activities of the PLP domains of human coronavirus NL63 and severe acute respiratory syndrome (SARS) coronavirus to determine if DUB activity mediates interferon antagonism. We found that NL63 PLP2 deconjugated ubiquitin (Ub) and the Ub-line molecule ISG15 from cellular substrates and processed both lysine-48- and lysine-63- linked polyubiquitin chains. This PLP2 DUB activity was dependent on an intact catalytic cysteine residue. We demonstrated that in contrast to PLP2 DUB activity, PLP2-mediated interferon antagonism did not require enzymatic activity. Furthermore, addition of an inhibitor that blocks coronavirus protease/DUB activity did not abrogate interferon antagonism. These results indicated that a component of coronavirus PLP-mediated interferon antagonism was independent of protease and DUB activity. Overall, these results demonstrate the multifunctional nature of the coronavirus PLP domain as a viral protease, DUB, and IFN antagonist and suggest that these independent activities may provide multiple targets for antiviral therapies.
HIV p24-specific helper T cell clones from immunized primates recognize highly conserved regions of HIV-1. (1990)

We have investigated Th cell recognition of the HIV core protein p24 by using CD4+ T cell clones derived from cynomolgus macaques immunized with hybrid HIV p24: Ty virus-like particles (VLP). T cell lines from two immunized animals responded to p24: Ty-VLP, control Ty-VLP, purified p24, and whole inactivated HIV, indicating the presence of T cells specific for p24 as well as the Ty carrier protein. The HIV determinants recognized by the T cell lines were identified by using a series of overlapping peptides synthesized according to the sequence of p24. Both T cell lines recognized peptide 11 (amino acids 235-249) and peptide 14 (amino acids 265-279). In addition, one T cell line also responded to peptide 9 (amino acids 215-229). Definitive identification of two T cell epitopes on p24 was confirmed at the clonal level: from a total of four T cell clones generated from one of the T cell lines, two respond specifically to peptide 11 and two to peptide 14. The T cell clones were CD4+ and MHC class II-restricted and secreted IL-2 in response to stimulation with purified p24, inactivated HIV or a single synthetic peptide. The specificity of the Th clones for variant peptides demonstrated cross-reactivity with two simian immunodeficiency virus isolates, but only limited responses to HIV-2 sequences. However, the Th cell epitopes identified on p24 are highly conserved between 12 HIV-1 isolates and were recognized by both of the immunized primates. These sequences may therefore be useful for priming a broadly reactive immune response to HIV-1.
Antagonistic effect of androgen on prostatic cell death (1984)

Androgen, besides having the well-established agonistic ability to stimulate prostate cell proliferation, also has an antagonistic ability to inhibit prostatic cell death. This statement is based upon the observations that 1) only 2.1% of the total prostatic cells die per day when serum testosterone level is sufficient for chronic maintenance of the gland; 2) 3 days following castration, when serum testosterone level is less than 10% of the intact value, the percentage of total prostatic cells now dying per day is increased tenfold to a value of 20.8%; and 3) this high rate of prostatic cell death can be inhibited following castration if serum androgen level is appropriately maintained by exogenous testosterone treatment. The serum testosterone level needed to antagonistically inhibit prostatic cell death (ie, 1.4 +/- 0.1 ng/ml) is more than twofold lower than that needed to antagonistically stimulate prostatic cell proliferation (3.3 +/- 0.4 ng/ml). Due to this dose difference, it is experimentally possible in castrated rats to inhibit prostatic cell death selectively without simultaneously stimulating cell proliferation and still completely prevent the rapid involution of the prostate following castration. These results suggest that the rapid involution of the prostate following castration is predominantly due to a decreased antagonistic effect of androgen on prostatic cell death rather than to a decreased agonistic effect of androgen on prostatic cell proliferation and that these two androgenic effects are distinct processes in the prostate.
HIF‐1α and CA IX staining in invasive breast carcinomas: Prognosis and treatment outcome (2007)

Abstract Hypoxia stabilizes HIF‐1α (Hypoxia Inducible Factor‐1α), which then triggers the expression of several genes involved in many aspects of cancer progression, including metabolic adaptation, cell survival and angiogenesis. The aim of our study was to evaluate the impact of HIF‐1α and CA IX (carbonic anhydrase IX) (one of its target genes) expression on prognosis and treatment outcome of patients with breast cancer. Because of the extreme O 2 ‐dependent instability of the protein, we first validated HIF‐1α staining using xenograft tumours that were subjected to experimental conditions mimicking surgical clamping or sitting at room temperature under normoxic conditions after surgical excision but before fixation. Afterwards, the immunohistochemical staining of HIF‐1α and CA IX was evaluated in 132 invasive breast carcinomas with a 10‐year follow‐up, and correlated to classical clinicopathological parameters and response to adjuvant therapy. No significant correlation was found between tumour size or nodal status and the expression of HIF‐1α or CA IX. Statistically significant association was found between HIF‐1α or CA IX staining and the grade, hormonal receptors loss and the presence of carcinoma in situ . Overexpression of HIF‐1α and CA IX correlates with a poor prognosis in breast cancer. We show that HIF‐1α is an independent prognostic factor for distant metastasis‐free survival and disease‐free survival in multivariate analysis. Furthermore, overexpression of HIF‐1α or CA IX correlates with a poor outcome after conventional adjuvant therapy. CA IX is, however, a weaker prognostic and predictive factor than HIF‐1α, and its association with HIF‐1α does not modify the survival curve neither response to therapy, compared to HIF‐1α alone. © 2006 Wiley‐Liss, Inc.
CDDO-Imidazolide inhibits growth and survival of c-Myc-induced mouse B cell and plasma cell neoplasms (2006)

Growth arrest and killing of neoplastic mouse B cells and plasma cells by CDDO-Im, a closely related derivative of the synthetic triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-28-oic acid, appears to be caused, in part, by drug-induced stress responses and reduction of Myc.
Early Determination of Reservoir Flow Units Using an Integrated Petrophysical Method (1997)

Abstract This paper uses case histories to introduce a graphical method for easily quantifying reservoir flow units based on geologic framework, petrophysical rock/pore types, storage capacity, flow capacity, and reservoir process speed. Using these parameters and four graphical tools, this paper outlines a quantitative approach to transform rock-type-based zonations into petrophysically based flow units that can be input into a numerical flow simulator. This method provides a tool for determining the minimum number of flow units to input into a numerical flow simulator that honors the foot-by-foot characteristics at the wellbore. A flow unit is a stratigraphically continuous interval of similar reservoir process speed that maintains the geologic framework and characteristics of rock types. Rock types are representative reservoir units with a distinct porosity-permeability relationship and a unique water saturation for a given height above free water level. The ideal data for this method is continuous core porosity, permeability, and saturation information drawn from throughout the entire reservoir. If such a data set is not available, it is necessary to calibrate wireline log data with core information to produce reliable estimates of porosity, permeability, and saturation. A full discussion of these data transforms are beyond the scope of this paper. The four graphical tools used to determine flow units are: Winland porosity-permeability cross plot, Stratigraphic Flow Profile (SFP), Stratigraphic Modified Lorenz Plot (SMLP) and Modified Lorenz Plot (MLP). This method begins by establishing rock types within a geologic framework. The geologic framework allows the flow units to be interpreted within a sequence stratigraphic model determining well-to-well correlation strategies. The key flow unit characteristics to be identified are barriers (seal to flow), speed zones (conduits), and baffles (zones that throttle fluid movement). This integrated, petrophysically based method of determining flow units has been successfully used in a wide array of reservoirs. We have applied it to young, unconsolidated sediments; structurally complex naturally fractured/vuggy carbonates; low permeability "tight" formation gas sands; diagenetically altered carbonates; complex mixed lithologies; and interbedded sand-shale sequences. The earlier in the life of a reservoir this process is used, the greater the understanding of future reservoir performance. This method allows the user to employ the least number of flow units and honor the character of the foot by foot data for simulation studies.
Role for α- l -fucosidase in the control of Helicobacter pylori -infected gastric cancer cells (2009)

Infecting about one-half of the global human population, Helicobacter pylori is well established as the primary cause of gastritis, duodenal ulcer, and gastric cancer. Currently there is no clear information regarding if and how host cells interact with H. pylori, and if such interactions are dependent on the type of gastric disease. Using fluorescently labeled fucose-containing glycoconjugates, we provide evidence observing both the uptake of L-fucose from gastric cancer cells to H. pylori and that human alpha-L-fucosidase 2 (FUCA2) is secreted only under coculture conditions (i.e., host cells infected with H. pylori). Upon depletion of FUCA2 by RNA interference and detection of translocated CagA (a virulence factor of H. pylori) in host cells, FUCA2 was found to be essential for H. pylori adhesion, in particular to the gastric cancer- and duodenal ulcer-specific strains. Additionally FUCA2 was shown to significantly enhance the expression of Lewis x antigen in H. pylori, which is critical for bacterial cell adhesion in the pathogenesis and defense strategy to escape host surveillance. These findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of H. pylori, but also support the idea that FUCA2 is a potential target for clinical diagnosis and therapeutic intervention of H. pylori-related diseases.
Dissociation of Lung Function Changes with Humoral Immunity during Inhaled Human Insulin Therapy (2006)

The small lung function changes observed with INH therapy are not mediated by the humoral immune response, or associated with acute decrements in lung function immediately after insulin inhalation.
Multiple-computer user interfaces (2000)

Graphical user interfaces (GUIs) are mainly designed for a single computer and a set of single input devices. However, when we simultaneously use many and different types of computers and electronic devices, such interfaces would often fail to work. As we can combine several tools to perform a task in the real world, we consider that it should be possible to dynamically combine multiple digital devices. It should also be possible to use "direct manipulation" interfaces that work across the boundary of these devices. We call this concept "multiple-computer user interfaces". This video demonstrates several interaction techniques based on this concept, including Pick-and-Drop, a digital whiteboard system with palmtop computers, and a digital table that can recognize objects placed on it.
Enzyme Replacement for Lysosomal Diseases (2005)

Following the demonstration of the nature of the enzymatic defects in the sphingolipid storage disorders in the mid-1960s, consideration was directed to the development of therapy for patients with these conditions. High on the list of possibilities was enzyme supplementation or replacement. Many years of arduous investigation and the development of novel protein targeting strategies were required to bring this concept to fruition. Enzyme replacement therapy (ERT) was eventually shown to be extraordinarily effective for patients with Gaucher disease, the most prevalent metabolic storage disorder of humans. Demonstration of the benefit of ERT in this disorder led to the extension of this approach to the treatment of other lysosomal storage disorders. This review presents the current status and anticipated developments in this field.
Preliminary protocol for interoperable telesurgery (2009)

In the same way Internet standards have connected heterogeneous computing systems, we predict robot communication standards will speed research and development of teleoperated robots. In this paper, a preliminary specification is presented for interoperability among robotic telesurgery systems. This is a first step towards developing a full telerobotics protocol. One way that interoperability will help is by allowing new teleoperation robots to be compared to existing ones on standard platforms. Using our preliminary specification, two telesurgical masters located in Yokohama, Japan controlled the same surgical assistant robot located in Seattle, WA, USA. Teleoperation was over the public Internet and users performance was measured on a standardized task. The value of the communication standard becomes clear, as a comparison between the two systems exposes several important improvements for the surgical master systems.
Recommender Systems for TV (1998)

We present research questions associated with recommender systems for TV and an example of such a
Biofuel Cells Controlled by Logically Processed Biochemical Signals: Towards Physiologically Regulated Bioelectronic Devices (2009)

Biofuel cells with a switchable power release controlled by biochemical signals, which can be logically processed by biomolecular computing systems, have been designed. The switchable properties of the biofuel cells were based on the polymer-brush-modified electrodes with the activity dependent on the solution pH value. The pH changes generated in situ by biocatalytic reactions allowed the reversible activation-inactivation of the bioelectrocatalytic interfaces-thus affecting the activity of the entire biofuel cells. Boolean logic operations performed by either enzyme- or immune-based systems were functionally integrated with the switchable biocatalytic process allowing logic control over them. Scaling up the complexity of the biocomputing systems to complex multi-component logic networks with a built-in "program" resulted in the control of the bioelectronic systems by multi-signal patterns of biochemical inputs. Future implantable biofuel cells producing electrical power on-demand depending on physiological conditions are feasible, as the result of the present research.
Renovator's delight: The return of the 'Deco Reno' seminar (2002)

One of the most frequent questions from new and prospective members concerns renovating their own Art Deco dwelling and Committee members are often asked to provide general advice on restoring examples of this architectural style and/or the source of appropriate building supplies.