gebhart/patrepeval huggingface dataset:
Comparison of galactolipase activity and free fatty acid levels in chloroplasts of chill-sensitive and chill-resistant plants (1987)

Galactolipase activity in chloroplasts of several chill-resistant plants was found to be very low [0.02-0.13 mumol free fatty acid (FFA) liberated min-1 mg protein-1] or not detected. The same phenomenon was observed for soybean and members of the Cucurbitaceae such as cucumber, pumpkin, melon and squash. Since, following cold storage of cucumber leaves, the levels of monogalactosyl-diacylglycerol and digalactosyl-diacylglycerol in chloroplasts decrease while those of FFA accumulate it seems likely that in these typical chill-sensitive plants galactolipase is present but inactivated during isolation procedure. The low galactolipase activity in chloroplasts was accompanied by a relatively low FFA content ranging from 0.05 mumol to 0.30 mumol FFA mg chlorophyll (Chl)-1. However, both pea and horse bean chloroplasts (with low galactolipase activity) exhibit about 0.45 mumol FFA mg Chl-1. Elevated galactolipase activity was observed in chloroplasts of most chill-sensitive species (ranging from 0.31 mumol to 1.32 mumol FFA liberated min-1 mg protein-1) as well as in chloroplasts from broad bean, a member of a chill-resistant species (1.26 mumol FFA liberated min-1 mg protein-1). In addition in this latter group of plants FFA level in chloroplasts often did not fit the galactolipase activity. The results suggest that there exists a tendency for chilling tolerance of plants to decrease both galactolipase activity and FFA level. However, in some plant species with elevated galactolipase activity the chloroplast FFA level does not correlate with enzyme activity.
DNA Vaccines against Human Immunodeficiency Virus Type 1 in the Past Decade (2004)

This article reviews advances in the field of human immunodeficiency virus type 1 (HIV-1) and AIDS vaccine development over the last decade, with an emphasis on the DNA vaccination approach. Despite the discovery of HIV-1 and AIDS in humans nearly 20 years ago, there is no vaccine yet that can prevent HIV-1 infection. The focus has shifted toward developing vaccines that can control virus replication and disease progression by eliciting broadly cross-reactive T-cell responses. Among several approaches evaluated, the DNA-based modality has shown considerable promise in terms of its ability to elicit cellular immune responses in primate studies. Of great importance are efforts aimed at improvement of the potency of this modality in the clinic. The review discusses principles of DNA vaccine design and the various mechanisms of plasmid-encoded antigen presentation. The review also outlines current DNA-based vaccine strategies and vectors that have successfully been shown to control virus replication and slow disease progression in animal models. Finally, it lists recent strategies that have been developed as well as novel approaches under consideration to enhance the immunogenicity of plasmid-encoded HIV-1 antigen in various animal models.
Squamous cell carcinoma of the cecum.A case report (1978)

Squamous cell carcinoma of the colon is a distinctly rare entity. Squamous cell carcinoma and adenoacanthoma occur in the colon and upper rectum 0.05% as frequently as adenocarcinoma. Squamous cell carcinoma of the cecum is an even rarer entity, this case appearing to be the seventh recorded case. A review of the literature concerning this topic is included in this report.
Two-Dimensional Protein Separation with Advanced Sample and Buffer Isolation Using Microfluidic Valves (2004)

Methods are described to achieve more efficient multidimensional protein separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength electrophoretic separations by active microvalve control in a microchip. Several experiments demonstrating independent 2D separation were performed, and critical parameters for optimal chip performance were identified, including channel passivation, electroosmosis control, and IEF linearity control. This strategy can be used for integration of different heterogeneous separation techniques, such as IEF, capillary electrophoresis, and liquid chromatography. This new device can be ideal for preseparation and preconcentration of complex biomolecule samples for a streamlined biomolecule analysis using mass spectrometry.
Idiotypic analysis of antibodies against the terpolymer L‐glutamic acid60‐L‐alanine30‐L‐tyrosine10 (GAT) IV. Induction of CGAT idiotype following immuniza‐ tion with various synthetic polymers containing glutamic acid and tyrosine (1979)

The immune responses of all inbred strains of mice specific to the synthetic terpolymer poly(LGlu60LAla30LTyr10), referred to as GAT10, are characterized by the presence of anti-GAT antibodies which share a common (CGAT) idiotype. In this report, we describe the ability of the synthetic polymers, LGlu33LAla33LTyr33, LGlu51-LAla34LTyr15 and poly-L(Tyr, Glu)-DLAla--LLys [(T,G)-A--L] to induce antibodies with CGAT idiotypic specificities. All of these polymers contain "GT"-related determinants. Following immunization with these polymers, antisera from responder mice bind the corresponding 125I-labeled antigen and 125I-labeled poly(LGlu50LTyr50) or GAT10. These antisera shared the CGAT idiotype which is associated with the antibody fraction with binding specificity for GAT10. Collectively, the present results indicate that GT-related determinants are required for the induction of the CGAT idiotype. Moreover, since the immune responses to these synthetic polymers are under distinct H-2-linked immune response (Ir) gene control, a mouse strain can be nonresponder to one polymer and responder to another; in this case, only the latter polymer induces CGAT idiotype. Thus, although the immune responses of inbred strains of mice to different polymers are under distinct Ir gene control, the antibody responses can be idiotypically related.
Comparison of refractory metal and silicide capping effects on aluminum metallizations (2003)

The effects of various refractory metal and silicide capping layers on aluminum metallizations are investigated. Ti-Al, Mo-Al, Ta-Al, WSi/sub 2/-Al, and MoSi/sub 2/-Al layered structures are compared in terms of electromigration and stress-induced voiding. The authors have found that the Ti-Al layered structure can suppress stress-induced void formation in underlying Al conductors. The effect of the Ti-Al layered structure on the suppression of stress-induced voiding can be attributed to the formation of the intermetallic compound Al/sub 3/Ti, which prevents plastic deformation of the film. Electromigration results indicate that the capping layers of refractory metals and silicides such as Ti, W, WSi/sub 2/, and MoSi/sub 2/ improve the mean time to failure by 4-10 times compared with Al without capping.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
APPRIS: annotation of principal and alternative splice isoforms (2012)

Here, we present APPRIS (http://appris.bioinfo.cnio.es), a database that houses annotations of human splice isoforms. APPRIS has been designed to provide value to manual annotations of the human genome by adding reliable protein structural and functional data and information from cross-species conservation. The visual representation of the annotations provided by APPRIS for each gene allows annotators and researchers alike to easily identify functional changes brought about by splicing events. In addition to collecting, integrating and analyzing reliable predictions of the effect of splicing events, APPRIS also selects a single reference sequence for each gene, here termed the principal isoform, based on the annotations of structure, function and conservation for each transcript. APPRIS identifies a principal isoform for 85% of the protein-coding genes in the GENCODE 7 release for ENSEMBL. Analysis of the APPRIS data shows that at least 70% of the alternative (non-principal) variants would lose important functional or structural information relative to the principal isoform.
Vaccinia Virus Late Transcription Is Activated in Vitro by Cellular Heterogeneous Nuclear Ribonucleoproteins (2001)

Vaccinia virus gene expression is temporally regulated, and three gene classes have been identified: early, intermediate, and late. Several virus-encoded proteins and an activity designated VLTF-X are required for maximum transcription in vitro of a template containing a late promoter. VLTF-X is present in both cytoplasmic and nuclear extracts prepared from uninfected mammalian cells and co-purifies with a late promoter DNA-binding activity. Here, extensive purification of VLTF-X has revealed that heterogeneous nuclear ribonucleoproteins A2/B1 and RBM3 co-purified with in vitro late transcription stimulation. Overexpression and purification of these proteins from Escherichia coli demonstrated that they both complemented for VLTF-X activity in in vitro transcription reactions. These studies identify two host cell factors potentially contributing to poxvirus replication in vivo.
You Are What You Tweet: Analyzing Twitter for Public Health (2021)

Analyzing user messages in social media can measure different population characteristics, including public health measures. For example, recent work has correlated Twitter messages with influenza rates in the United States; but this has largely been the extent of mining Twitter for public health. In this work, we consider a broader range of public health applications for Twitter. We apply the recently introduced Ailment Topic Aspect Model to over one and a half million health related tweets and discover mentions of over a dozen ailments, including allergies, obesity and insomnia. We introduce extensions to incorporate prior knowledge into this model and apply it to several tasks: tracking illnesses over times (syndromic surveillance), measuring behavioral risk factors, localizing illnesses by geographic region, and analyzing symptoms and medication usage. We show quantitative correlations with public health data and qualitative evaluations of model output. Our results suggest that Twitter has broad applicability for public health research.
A comparison of classical, robust, and decentralized control designs for multiple power system stabilizers (2000)

Several control design techniques, namely, the classical phase compensation approach, the /spl mu/-synthesis, and a linear matrix inequality technique, are used to coordinate two power system stabilizers to stabilize a 5-machine equivalent of the South/Southeast Brazilian system. The open-loop system has an unstable intearea mode and cannot be stabilized using only one conventional power system stabilizer. Both centralized and decentralized controllers are considered. The different designs are compared and several interesting observations are provided.
Time-resolved and steady-state fluorescence spectroscopy from bacteria subjected to bactericidal agents (2004)

The time-resolved and steady-state changes in fluorescence were investigated from one spore-forming (<i>Bacillus subtilis</i>) and four non-spore forming (<i>Escherichia coli, Staphylococcus aureus, Enterococcus faecalis</i>, and <i>Pseudomonas aeruginosa</i>) bacteria subjected to different bactericidal agents. The bactericidal agents were sodium hypochlorite (bleach) hydrogen peroxide, formaldehyde, and UV light exposure. Application of sodium hypochlorite resulted in an almost total lose of fluorescence signal and large decrease in the optical density of the bacterial suspension. Addition of hydrogen peroxide resulted in a 35% decrease in emission intensity fom the Sa and an 85-95% decrease for the other bacteria. Ultraviolet light exposure resulted in a 5-35% decrease in the emission intensity of the tryptophan band. The addition of formaldehyde to the bacteria did not result in significant changes in the steady-state emission intensity, but did shift the tryptophan emission peak position to shorter wavelengths by 3 to 5 nm. Time-resolved fluorescence measurements showed that the fluorescence lifetime of tryptophan in the bacteria could not be described by a single exponential decay, and was similar to that of tryptophan in neutral aqueous solution. Upon addition of formaldehyde to the Gram positive bacteria (Bs and Sa) the strength of the short lifetime component increased dramatically, while for the Gram negative bacteria, a smaller increase was observed. These fluorescence changes reflect the different mechanisms of the bactericidal agents and may provide a useful tool to monitor the effectiveness of disinfectants.
A Miniaturized Low-Power Wireless Remote Environmental Monitoring System using Microfabricated Electrochemical Sensing Electrodes (2003)

This work has been supported by the Korea Science and Engineering Foundation (KOSEF) through the MICROS center at KAIST. The authors would like to thank Myungsoo Kim for antenna design.
Consequences of Brief Ischemia: Stunning, Preconditioning, and Their Clinical Implications (2001)

In experimental studies in the dog, total proximal coronary artery occlusions of up to 15 minutes result in reversible injury, meaning that the myocytes survive this insult. The 15 minutes of ischemia, however, induce numerous changes in the myocardium, including certain monuments to the brief episode of ischemia that may persist for days. One of these monuments is stunned myocardium, which represents "prolonged postischemic contractile dysfunction of myocardium salvaged by reperfusion." The mechanism of stunning involves generation of oxygen radicals as well as alteration in calcium homeostasis and possibly alteration in contractile protein structure. Stunning has been observed in several clinical scenarios, including after percutaneous transluminal coronary angioplasty, unstable angina, stress-induced ischemia, after thrombolysis, and after cardiopulmonary bypass. Oxygen radical scavengers and calcium channel blockers have been shown to enhance function of stunned myocardium in experimental studies, and in a few clinical studies, calcium channel blockers have been shown to ameliorate stunning. Although brief periods of ischemia can contribute to prolonged left ventricular dysfunction and even heart failure, they paradoxically play a cardioprotective role. Episodes of ischemia as short as 5 minutes, followed by reperfusion, protect the heart from a subsequent longer coronary artery occlusion by markedly reducing the amount of necrosis that results from the test episode of ischemia. This phenomenon, called ischemic preconditioning, has been observed in virtually every species in which it has been studied and is a powerful cardioprotective effect. The mechanism of ischemic preconditioning involves both triggers and mediators and involves complex second messenger pathways that appear to involve such components as adenosine, adenosine receptors, the epsilon isoform of protein kinase C, the ATP-dependent potassium channels, as well as others, including a paradoxical protective role of oxygen radicals. Both an early and a late phase of preconditioning have been described, and the mechanisms underlying their induction are under investigation. That preconditioning may occur in humans is suggested by the observations that repetitive balloon inflations in the coronary artery are associated with progressively less chest pain, ST-segment elevation, lactate production, the protective effects of preinfarction angina, the anginal "warm-up phenomenon," and studies performed on human cardiac biopsies that show metabolic properties suggesting preconditioning. Development of pharmacological agents that stimulate second messenger pathways thought to be involved in preconditioning, but without causing ischemia, could result in novel approaches to treating ischemia. Hence, on one hand, brief episodes of ischemia can have a negative effect on the heart: stunning; and on the other hand, they have a protective effect: preconditioning. The future challenge is how to minimize the stunning phenomenon and maximize the preconditioning phenomenon in clinical practice.
LACK OF SIGNIFICANT PERSON-TO-PERSON SPREAD OF SWINE INFLUENZA-LIKE VIRUS FOLLOWING FATAL INFECTION IN AN IMMUNOCOMPROMISED CHILD (1984)

In February 1982, a four-year-old Nevada girl with acute lymphoblastic leukemia in remission was hospitalized with fulminant pneumonia and died eight days later at a hospital in California. An influenza virus was the only pathogen detected, and was present in both antemortem and postmortem specimens. The virus was closely related antigenically to A/New Jersey/8/76 (H1N1) and had a genome very similar to a contemporary enzootic swine influenza virus. The patient had had no known contact with swine, and the source of infection could not be determined. Only five possible secondary cases could be detected by retrospective investigation of 62 contacts, and there was no evidence of spread to the general community. Swine influenza viruses circulate among pigs in the United States annually, and it is likely that sporadic transmissions to humans will continue to be detected. Nevertheless, person-to-person spread under these circumstances appears to be limited.
Hidden Thermodynamics of Mutant Proteins: A Molecular Dynamics Analysis (1989)

A molecular dynamics simulation method is used to determine the contributions of individual amino acid residues and solvent molecules to free energy changes in proteins. Its application to the hemoglobin interface mutant Asp G1(99) beta----Ala shows that some of the contributions to the difference in the free energy of cooperativity are as large as 60 kilocalories (kcal) per mole. Since the overall free energy change is only -5.5 kcal/mole (versus the experimental value of -3.4 kcal/mole), essential elements of the thermodynamics are hidden in the measured results. By exposing the individual contributions, the free energy simulation provides new insights into the origin of thermodynamic changes in mutant proteins and demonstrates the role of effects beyond those usually considered in structural analyses.
Reengineering Granulocyte Colony-stimulating Factor for Enhanced Stability (2001)

Granulocyte colony-stimulating factor is a long-chain cytokine that has both biological and therapeutic applications. It is involved in the production and maturation of neutrophilic progenitor cells and neutrophils and is administered to stimulate the production of white blood cells to reduce the risk of serious infection in immunocompromised patients. We have reengineered granulocyte colony-stimulating factor to improve the thermodynamic stability of the protein, focusing on enhancing the alpha-helical propensity of residues in the antiparallel 4-helix bundle of the protein. These redesigns resulted in proteins with substantially enhanced stability while retaining wild-type levels of biological activity, measured as the ability of the reengineered proteins to stimulate the proliferation of murine myeloid cells transfected with the granulocyte colony-stimulating factor receptor.
Addition of Vinylketenes to Enamines. A Method for the Preparation of 6,6‐Dialkylcyclohexa‐2,4‐dienones and 4,4‐Dialkyl‐2‐vinylcyclobutenones (1982)

Addition von Vinylketenen an Enamine. Eine Methode zur Herstellung von 6,6‐Dialkylcyclohexa‐2,4‐dienonenund4,4‐Dialkyl‐2‐vinylcyelobutenonen Drei Enamine ( 1 – 3 ) wurden mit fünf Vinylketenen ( 5a – 5e ) ( s. Schema 2 ) zur Reaktion gebracht. Die Vinylketene wurden in situ durch HCl‐Eliminierung aus α,β‐ungesättigten Säurechloriden mit Triäthylamin hergestellt. Die Cycloadditionen von 1 – 3 an 5a – 5e führten zu 6,6‐Dialkyl‐5‐dialkylaminoeyclohex‐2‐enonen (kurz: Cyclohexenone) bzw. zu 3‐Dialkylamino‐4,4‐dimethyl‐2‐vinylcyclobutanonen (kurz: Vinylcyclobutanone) oder zu einem Gemisch der beiden, je nach Natur der Partner bzw. des Lösungsmittels (s. Tab. 1 ). Durch oxydative Amin‐Eliminierung wurden die Cyclohexenone in 6,6‐Dialkylcyclohexa‐2,4‐dienone und die Vinylcyclobutanone in 4,4‐Dialkyl‐2‐vinylcyclobutenone übergefuhrt. Diese Reaktion stellt eine einfache Synthese von verschieden substituierten 6.6‐Dialkylcyclohexa‐2,4‐dienonen bzw. 4,4‐Dialkyl‐2‐vinylcyclobutenonen (siehe Schema 1 ) dar.
Climate Change 2014 - Synthesis Report (2015)

The Synthesis Report (SYR) distils and integrates the findings of the three Working Group contributions to the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC), the most comprehensive assessment of climate change undertaken thus far by the IPCC: Climate Change 2013: The Physical Science Basis; Climate Change 2014: Impacts, Adaptation, and Vulnerability; and Climate Change 2014: Mitigation of Climate Change. The SYR also incorporates the findings of two Special Reports on Renewable Energy Sources and Climate Change Mitigation (2011) and on Managing the Risks of Extreme Events and Disasters to Advance Climate Change Adaptation (2011).
Chemical Recycling of Carbon Dioxide to Methanol and Dimethyl Ether: From Greenhouse Gas to Renewable, Environmentally Carbon Neutral Fuels and Synthetic Hydrocarbons (2008)

Nature's photosynthesis uses the sun's energy with chlorophyll in plants as a catalyst to recycle carbon dioxide and water into new plant life. Only given sufficient geological time can new fossil fuels be formed naturally. In contrast, chemical recycling of carbon dioxide from natural and industrial sources as well as varied human activities or even from the air itself to methanol or dimethyl ether (DME) and their varied products can be achieved via its capture and subsequent reductive hydrogenative conversion. The present Perspective reviews this new approach and our research in the field over the last 15 years. Carbon recycling represents a significant aspect of our proposed Methanol Economy. Any available energy source (alternative energies such as solar, wind, geothermal, and atomic energy) can be used for the production of needed hydrogen and chemical conversion of CO(2). Improved new methods for the efficient reductive conversion of CO(2) to methanol and/or DME that we have developed include bireforming with methane and ways of catalytic or electrochemical conversions. Liquid methanol is preferable to highly volatile and potentially explosive hydrogen for energy storage and transportation. Together with the derived DME, they are excellent transportation fuels for internal combustion engines (ICE) and fuel cells as well as convenient starting materials for synthetic hydrocarbons and their varied products. Carbon dioxide thus can be chemically transformed from a detrimental greenhouse gas causing global warming into a valuable, renewable and inexhaustible carbon source of the future allowing environmentally neutral use of carbon fuels and derived hydrocarbon products.