gebhart/patrepeval huggingface dataset:
Normal Cells, but Not Cancer Cells, Survive Severe Plk1 Depletion (2006)

We previously reported the phenotype of depletion of polo-like kinase 1 (Plk1) using RNA interference (RNAi) and showed that p53 is stabilized in Plk1-depleted cancer cells. In this study, we further analyzed the Plk1 depletion-induced phenotype in both cancer cells and primary cells. The vector-based RNAi approach was used to evaluate the role of the p53 pathway in Plk1 depletion-induced apoptosis in cancer cells with different p53 backgrounds. Although DNA damage and cell death can occur independently of p53, p53-deficient cancer cells were much more sensitive to Plk1 depletion than cancer cells with functional p53. Next, the lentivirus-based RNAi approach was used to generate a series of Plk1 hypomorphs. In HeLa cells, two weak hypomorphs showed only slight G2/M arrest, a medium hypomorph arrested with 4N DNA content, followed later by apoptosis, and a strong Plk1 hypomorph underwent serious mitotic catastrophe. In well-synchronized HeLa cells, a medium level of Plk1 depletion caused a 2-h delay of mitotic progression, and a high degree of Plk1 depletion significantly delayed mitotic entry and completely blocked cells at mitosis. In striking contrast, normal hTERT-RPE1 and MCF10A cells were much less sensitive to Plk1 depletion than HeLa cells; no apparent cell proliferation defect or cell cycle arrest was observed after Plk1 depletion in these cells. Therefore, these data further support suggestions that Plk1 may be a feasible cancer therapy target.
Scientific Charge-Coupled Devices (2001)

The invention of the charge-coupled device 30 years ago was the beginning of a remarkable image capture technology that has changed the course of imaging in fields ranging from astronomy to biotechnology. This book presents a comprehensive history, tutorial, and state-of-the-art description of CCDs and is intended for scientists, engineers, imaging hardware managers, and graduate students.
Activity of Three Disinfectants and Acidified Nitrite AgainstClostridium difficileSpores (2003)

Cidal activity was shown against C. difficile spores by glutaraldehyde, peracetyl ions, and acidified nitrite. As acidified nitrite and peracetyl ions are considered to be environmentally safe chemicals, these agents seem well suited for the disinfection of C. difficile spores in the hospital environment.
Internet Engineering Task Force (IETF) (2015)

This document describes an extension to the basic IP fast reroute mechanism, described in RFC 5286, that provides additional backup connectivity for point-to-point link failures when none can be provided by the basic mechanisms.
Isolation and Characterization of Two Safflower Oleoyl-Acyl Carrier Protein Thioesterase cDNA Clones (1992)

Oleoyl-acyl carrier protein (18:1-ACP) thioesterase has been partially purified from developing safflower (Carthamus tinctorius) seeds. Protein species with molecular masses of 34 and 40 kD associated with thioesterase activity were identified and partially sequenced. Analysis of amino-terminal and internal cyanogen bromide peptide sequences revealed no differences in the primary structure of the two species. Amino acid sequence was used to design degenerate oligonucleotides for primers in a polymerase chain reaction (PCR) using safflower embryo cDNA as a template. A 380-base pair PCR product was used to isolate two classes of cDNA clones, designated 2-1 and 5-2, from the embryo cDNA library. Clone 2-1 encodes a 389-amino acid protein including a 60-amino acid transit peptide, and contains all of the protein sequence determined from the 34- and 40-kD proteins. Clone 5-2 encodes a 385-amino acid protein with 80% identity to that encoded by 2-1. Expression of the two safflower cDNA clones in Escherichia coli resulted in a 50- to 100-fold increase in the level of 18:1-ACP thioesterase activity. Both thioesterases are most active on 18:1-ACP; however, the enzyme encoded by 5-2 shows less discrimination against saturated 16- and 18-carbon acyl-ACP substrates.
Visual touchpad (2004)

This paper presents the Visual Touchpad, a low-cost vision-based input device that allows for fluid two-handed interactions with desktop PCs, laptops, public kiosks, or large wall displays. Two downward-pointing cameras are attached above a planar surface, and a stereo hand tracking system provides the 3D positions of a user's fingertips on and above the plane. Thus the planar surface can be used as a multi-point touch-sensitive device, but with the added ability to also detect hand gestures hovering above the surface. Additionally, the hand tracker not only provides positional information for the fingertips but also finger orientations. A variety of one and two-handed multi-finger gestural interaction techniques are then presented that exploit the affordances of the hand tracker. Further, by segmenting the hand regions from the video images and then augmenting them transparently into a graphical interface, our system provides a compelling direct manipulation experience without the need for more expensive tabletop displays or touch-screens, and with significantly less self-occlusion.
Sulfotransferases of Two Specificities Function in the Reconstitution of High Endothelial Cell Ligands for L-selectin (1999)

L-selectin, a lectin-like receptor, mediates rolling of lymphocytes on high endothelial venules (HEVs) in secondary lymphoid organs by interacting with HEV ligands. These ligands consist of a complex of sialomucins, candidates for which are glycosylation- dependent cell adhesion molecule 1 (GlyCAM-1), CD34, and podocalyxin. The ligands must be sialylated, fucosylated, and sulfated for optimal recognition by L-selectin. Our previous structural characterization of GlyCAM-1 has demonstrated two sulfation modifications, Gal-6-sulfate and GlcNAc-6-sulfate in the context of sialyl Lewis x. We now report the cloning of a Gal-6-sulfotransferase and a GlcNAc-6-sulfotransferase, which can modify GlyCAM-1 and CD34. The Gal-6-sulfotransferase shows a wide tissue distribution. In contrast, the GlcNAc-6-sulfotransferase is highly restricted to HEVs, as revealed by Northern analysis and in situ hybridization. Expression of either enzyme in Chinese hamster ovary cells, along with CD34 and fucosyltransferase VII, results in ligand activity, as detected by binding of an L-selectin/IgM chimera. When coexpressed, the two sulfotransferases synergize to produce strongly enhanced chimera binding.
Global Prevalence of Presbyopia and Vision Impairment from Uncorrected Presbyopia (2018)

There is a significant burden of VI from uncorrected presbyopia, with the greatest burden in rural areas of low-resource countries.
Enhanced waveform interpolative coding at low bit-rate (2001)

This paper presents a high quality enhanced waveform interpolative (EWI) speech coder at low bit-rate. The system incorporates novel features such as optimization of the slowly evolving waveform (SEW) for interpolation, analysis-by-synthesis (AbS) vector quantization (VQ) of the SEW dispersion phase, dual-predictive AbS quantization of the SEW, efficient parameterization of the rapidly-evolving waveform (REW) magnitude, and VQ of the REW parameter, a special pitch search for transitions, and switched-predictive analysis-by-synthesis gain VQ. Subjective tests indicate that the 2.8 kb/s EWI coder's quality exceeds that of G.723.1 at 5.3 kb/s, and it is slightly better than that of G.723.1 at 6.3 kb/s.
Robust extended multidelay filter and double-talk detector for acoustic echo cancellation (2006)

We propose an integrated acoustic echo cancellation solution based on a novel class of efficient and robust adaptive algorithms in the frequency domain, the extended multidelay filter (EMDF). The approach is tailored to very long adaptive filters and highly auto-correlated input signals as they arise in wideband full-duplex audio applications. The EMDF algorithm allows an attractive tradeoff between the well-known multidelay filter and the recursive least-squares algorithm. It exhibits fast convergence, superior tracking capabilities of the signal statistics, and very low delay. The low computational complexity of the conventional frequency-domain adaptive algorithms can be maintained thanks to efficient fast realizations. We also show how this approach can be combined efficiently with a suitable double-talk detector (DTD). We consider a corresponding extension of a recently proposed DTD based on a normalized cross-correlation vector whose performance was shown to be superior compared to other DTDs based on the cross-correlation coefficient. Since the resulting DTD also has an EMDF structure it is easy to implement, and the fast realization also carries over to the DTD scheme. Moreover, as the robustness issue during double talk is particularly crucial for fast-converging algorithms, we apply the concept of robust statistics into our extended frequency-domain approach. Due to the robust generalization of the cost function leading to a so-called M-estimator, the algorithms become inherently less sensitive to outliers, i.e., short bursts that may be caused by inevitable detection failures of a DTD. The proposed structure is also well suited for an efficient generalization to the multichannel case
New Organic Chemistry of Sulfur Dioxide (2007)

Simple 1,3-dienes undergo highly stereoselective hetero-Diels-Alder additions with SO2 at low temperature giving sultines. These reactions that are faster than the more exothermic cheletropic additions of SO2-producing sulfolenes. This has led to the invention of a new C-C bond-forming reaction combining electron-rich dienes and alkenes with SO2. The reaction cascade has been exploited to develop combinatorial, one-pot, four-component syntheses of polyfunctional sulfones, sulfonamides, and sulfonic esters. It also allows us to generate, in one-pot operations, enantiomerically enriched polypropionate fragments containing up to three contiguous stereogenic centers and a (E)-alkene unit. These fragments can be used directly in further C-C bond-forming reactions, such as cross-aldol condensations, thus permitting the expeditious construction of complicated natural products of biological interest (e.g., Baconipyrones, Rifamycin S, Apoptolidinone) and analogues. New ene reactions of SO2 have also been discovered; they open new avenues to organic synthesis.
CRT colorimetry. Part II: Metrology (1993)

Abstract The colorimetric characterization of computer‐controlled CRT displays require radiometric measurements with high precision and accuracy in order to achieve acceptable colorimetric accuracy in defining stimuli generated with this type of imaging modality. Precision and accuracy requirements for photometers, colorimeters, and spectroradiometers were evaluated. When models are used to relate digital counts defining a stimulus and resulting spectral radiant exitance, displays are assumed to exhibit channel and spatial independence. A variety of tests and the results of evaluating four imaging systems are described. From these analyses, measurement accuracy is mainly limited by wavelength scale in the case of spectro‐radiometers and filter fit in the case of colorimeters. Measurement precision is limited by the number of significant figures for fixed‐range devices and signal‐to‐noise limitations for low‐luminance stimuli. Display accuracy is limited by a lack of channel and spatial independence. Display precision is mainly limited by the electronic design of the display and the stability and load independence of the gun amplifiers. © 1993 John Wiley &amp; Sons, Inc.
Localization of the IgG effector site for monocyte receptors. (1975)

A peptide consisting of 10 amino acids derived from the CH3 region of human IgG was shown to bind to monocytes and to inhibit rosette formation of antibody-coated erythrocytes with human monocytes. Two myeloma proteins of the IgG1 and IgG3 subclass, both with known deletions in the CH2 region of the gamma chain, showed unimpaired ability to bind to monocytes. These experiments suggest that the isolated peptide represents the primary site of attachment of IgG to monocytes.
Electrical signal processing techniques in long-haul fiber-optic systems (1990)

The potential for electrical signal processing to mitigate the effect of intersymbol interference in long-haul fiber-optic systems is discussed. Intersymbol interference can severely degrade performance and consequently limit both the maximum distance and data rate of the system. Several techniques for reducing intersymbol interference in single-mode fiber systems with single-frequency lasers are presented, and those techniques which are appropriate at high data rates in direct coherent detection systems are identified. The performances of linear equalization (tapped delay lines), nonlinear cancellation (variable threshold detection), maximum-likelihood detection, coding, and multilevel signaling are analyzed. The results for a simulated binary 8-Gb/s system show that simple techniques can be used to reduce intersymbol interference substantially, thereby increasing the system margin by several decibels. A six-tap linear equalizer increases the dispersion-limited distance (due to chromatic or polarization dispersion) by 20% (or reduces the optical power penalty by as much as a factor of two) in direct detection systems, even when the distortion is nonlinear. A nonlinear cancellation technique (adjusting the decision threshold in the detector based on previously detected bits) can more than double the dispersion-limited distance and/or data rate.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Recent Developments in Accelerated Cheese Ripening (1991)

Strategies for accelerating the ripening of cheese have traditionally included the use of enzymes like proteinases, peptidases, and lipases. These approaches have shown that the addition of free enzymes to milk or cheese led to uncontrolled biochemical reactions. Consequently, during recent years, new approaches have been attempted to accelerate the cheese ripening process. The addition of liposome-entrapped enzymes and physically or genetically modified lactic bacteria to cheese milk seem to provide some definite advantages. This article reviews the developments in the last 5 yr in this domain dealing with both traditional and untraditional approaches. (Key words:
Fixed-lag smoother for digital channel equalisation (1977)

A new equaliser for digital communication channels, which carries out a discrete-time fixed-lag smoothing of the state vector of the channel, modelled as a tapped delay line of finite length, is proposed. The smoothed estimate uses and improves the filtered estimate of the conventional Kalman-filter equaliser.
Enhanced secondary electron injection in novel SiGe flash memory devices (2002)

The property of SiGe layers to control channel-initiated secondary electron (CHISEL) gate current is explored for the first time using 2-D Monte Carlo simulation. Novel planar and vertical SiGe flash memory cells show a 4/spl times/ enhancement in CHISEL injection, with up to 93% of gate electrons generated in SiGe layers.
Efficient Monitoring of Enzymatic Conjugation Reaction by Surface-Enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry for Process Optimization (2006)

Efficient analysis of bioconjugation reactions is one the most challenging task for optimizing and eventually achieving the reproducible production of large amount of conjugates. In particular, the complexity of some reaction mixtures precludes the use of most of the existing methods, because of the presence of large amounts of contaminants. As an alternative method, we used surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) for monitoring an in vitro enzymatic transglycosylation of N-acetylgalactosamine (GalNAc) residues to a recombinant mucin protein MUC6. For this reaction, catalyzed by the uridine 5'-diphospho-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts), we used either a recombinant ppGalNAc-T1 or a mixture of ppGalNAc-Ts contained in MCF7 tumor cell extracts. In the present study, we show that SELDI-TOF MS offers unique advantages over the traditional methodologies. It is a rapid, accurate, sensitive, reproducible, and very convenient analytical method for monitoring the course of a bioconjugation, even in heterogeneous samples such as cell extracts. SELDI-TOF MS proved very useful for optimizing the reaction parameters of the transglycosylation and for achieving the large scale preparation of Tn antigen-glycosylated mucins for antitumor immunotherapy applications.
Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay (1993)

A polymerase chain reaction (PCR)-coupled ligase chain reaction (LCR) assay for the specific detection of Listeria monocytogenes (M. Wiedmann, J. Czajka, F. Barany, and C. A. Batt, Appl. Environ. Microbiol. 58:3443-3447, 1992) has been modified for detection of the LCR products with a nonisotopic readout. When a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the LCR products was used, the PCR-coupled LCR gave a sensitivity of 10 CFU of L. monocytogenes. The detection method with the chemiluminescent substrate Lumi-Phos 530 permitted detection of the LCR products in less than 3 h, so that the whole assay can be completed within 10 h.
Recombinant Mycobacterium bovis Bacillus Calmette-Guerin Elicits Human Immunodeficiency Virus Type 1 Envelope-Specific T Lymphocytes at Mucosal Sites (2007)

A successful vaccine vector for human immunodeficiency virus type 1 (HIV-1) should induce anti-HIV-1 T-cell immune responses at mucosal sites. We have constructed recombinant Mycobacterium bovis bacillus Calmette-Guérin (rBCG) expressing an HIV-1 group M consensus envelope (Env) either as a surface, intracellular, or secreted protein as an immunogen. rBCG containing HIV-1 env plasmids engineered for secretion induced optimal Env-specific T-cell gamma interferon enzyme-linked immunospot responses in murine spleen, female reproductive tract, and lungs. While rBCG-induced T-cell responses to HIV-1 envelope in spleen were lower than those induced by adenovirus prime/recombinant vaccinia virus (rAd-rVV) boost, rBCG induced comparable responses to rAd-rVV immunization in the female reproductive tract and lungs. T-cell responses induced by rBCG were primarily CD4(+), although rBCG alone did not induce anti-HIV-1 antibody. However, rBCG could prime for a protein boost by HIV-1 envelope protein. Thus, rBCG can serve as a vector for induction of anti-HIV-1 consensus Env cellular responses at mucosal sites.
Effects of Melanocortin Receptor Activation and Blockade on Ethanol Intake: A Possible Role for the Melanocortin-4 Receptor (2005)

The present results suggest that MC4R, and not MC3R, modulates MCR agonist-induced reduction of ethanol consumption and that ethanol intake is increased by the antagonistic actions of AgRP-(83-132). These findings strengthen the argument that MCR signaling controls ethanol consumption and that compounds directed at MCR may represent promising targets for treating alcohol abuse disorders in addition to obesity.
Extracellular Toll-Like Receptor 2 Region Containing Ser40-Ile64 but Not Cys30-Ser39 Is Critical for the Recognition ofStaphylococcus aureus Peptidoglycan (2001)

Toll-like receptor 2 (TLR2) and CD14 function as pattern recognition receptors for bacterial peptidoglycan (PGN). TLRs and CD14 possess repeats of the leucine-rich motif. To address the role of the extracellular domain of TLR2 in PGN signaling, we constructed CD14/TLR2 chimeras, in which residues 1-356 or 1-323 of CD14 were substituted for the extracellular domain of TLR2, and five deletion mutants of TLR2, in which the progressively longer regions of extracellular TLR2 regions were deleted. PGN induced NF-kappaB activation in HEK293 cells expressing TLR2 but not in cells expressing CD14/TLR2 chimeras. The cells transfected with a deletion mutant TLR2(DeltaCys30-Ile64) as well as TLR2(DeltaCys30-Asp160) and TLR2(DeltaCys30-Asp305) failed to respond to PGN, indicating the importance of the TLR2 region Cys(30)-Ile(64). Although TLR2(DeltaCys30-Ser39) conferred cell responsiveness to PGN, the cells expressing TLR2(DeltaSer40-Ile64) failed to induce NF-kappaB activation. In addition, NF-kappaB activity elicited by PGN was significantly attenuated in the presence of synthetic peptide corresponding to the TLR2 region Ser(40)-Ile(64). From these results, we conclude that; 1) CD14 cannot functionally replace the extracellular domain of TLR2 in PGN signaling; 2) the TLR2 region Cys(30)-Ser(39) is not required for PGN recognition; 3) the TLR2 region containing Ser(40)-Ile(64) is critical for PGN recognition.
Kinetics of amine‐cyclic anhydride reactions in moderately polar solutions (1995)

Abstract The validity of extrapolating the reactivity of low molar mass compounds in solution to the polymer‐analogous chemistry between polymer‐bound functionality is investigated for the reaction of primary amines with cyclic anhydrides in the moderately polar solvents, anisole and tetraethyleneglycol dimethylether. The kinetics of amic‐acid formation and imidization of polymeric and small molecule mixtures measured by Fourier‐Transform Infrared Spectroscopy at near‐ambient and elevated temperatures are compared. A Significant decrease in both reaction rates is observed upon changing the primary amine from aliphatic to benzylic, benzylic to 1,2‐diphenylethylamine, and 1,2‐diphenylethylamine to polystyrene‐bound 1,2‐diphenylethylamine. Reasons for the influence of polymer‐bound chemical functionality on the reaction rates for these amines are discussed. The imidization step is found to be rate limiting in the reaction of phthalic anhydride with benzylamine at the functional group concentrations reported (0.14 M or less). © 1995 John Wiley &amp; Sons, Inc.
Thalidomide Inhibits Activation of Caspase-1 (2009)

Thalidomide is an efficient anti-inflammatory and anti-angiogenic drug, but its therapeutic use is problematic due to a strong teratogenic activity. Nevertheless, thalidomide was approved for the treatment of inflammatory skin diseases and certain types of cancer, and it is extensively tested for several other indications. Recently, we demonstrated that active caspase-1, whose activation is dependent on inflammasome complexes, is required for unconventional protein secretion of proinflammatory cytokines such as IL-1 and of the proangiogenic fibroblast growth factor 2. In this study, we show that pharmacological doses of thalidomide strongly reduced the secretion of both proteins. Thalidomide-treated cells also released less of other leaderless proteins, which require caspase-1 activity for their secretion. In line with these findings, the drug inhibited activation and activity of caspase-1 in cultured cells but not in vitro. The latter finding suggests that the pharmacological activity is exerted by a metabolite of the drug. The anti-inflammatory activity of thalidomide was also mediated via caspase-1 in mice. These findings represent a novel mechanism by which thalidomide exerts its pharmacological activity and suggest that inhibition of the activity of IL-1 might represent a novel strategy to substitute thalidomide.