gebhart/patrepeval huggingface dataset:
[Dental pulp in carious teeth]. (1992)

Various etiologic causes lead to dental pulp changes ranging from inflammation to necrosis. The most frequent changes, however, are caused by bacterial activity and toxins from carious process. According to the opinion of various authors and on the basis of our investigations the conclusion can be made that all the forms of dental pulp changes can be developed as the sequela of the disease of supporting apparatus. Pathohistologic examination of dental pulp of cariously damaged teeth showed that the occurred changes are mostly the sequela of inflammation and regressive and progressive changes are found as well.
Leaf essential oils of the genusLeptospermum (Myrtaceae) in eastern Australia, Part 6.Leptospermum polygalifolium and allies (2000)

The leaf essential oils of Leptospermum polygalifolium and related species were isolated and examined. L. morrisonlii produced an essential oil in which the principal component was the β-triketone grandiflorone, while in L. oreophilum the principal component was (E,E)-farnesol. L. variabile gave oils with a spread of composition, the majority of samples being rich in geranyl acetate, β-caryophyllene and humulene, while another sample was rich in 1,8-cineole and a further sample contained comparable amounts of α-pinene, β-caryophyllene and α-, β- and γ-eudesmol. Leptospermum sp. (Mt Maroon, A.R. Bean 6665) appeared to be a chemically variable species; one specimen showed β-caryophyllene and humulene as principal components, while a second gave an oil rich in sesquiterpenes with β-caryophyllene, δ-cadinene, calamenene and an unidentified sesquiterpene hydrocarbon being the major contributors. Of the seven subspecies of L. polygalifolium, ssp. polygalifolium, montanum and howense contained oils which were rich in α-, β, and γ-eudesmol. These were the only subspecies to contain these compounds in more than trace amounts. Of the remaining four subspecies, ssp. cismontanum, transmontanum, tropicum and ‘wallum’, all contained significant amounts of 1,8-cineole. They usually contained larger quantities of spathulenol. All seven subspecies contained α-pinene in significant amounts, while all but spp. howense also contained β-pinene, usually in comparable amounts. Leptospermum madidum spp. sativum gave an oil rich in monoterpenes, with α-pinene, β-pinene, and γ-terpinene being the major components. α-, β-, and γ-eudesmol also made significant contributions to the oil. Copyright © 2000 John Wiley & Sons, Ltd.
Tissue Engineering Using Synthetic Biodegradable Polymers (1993)

ADVERTISEMENT RETURN TO BOOKPREVChapterNEXTTissue Engineering Using Synthetic Biodegradable PolymersC. A. VacantiC. A. Vacanti Department of Orthopaedic Surgery and Anesthesiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02115More by C. A. Vacanti, J. P. VacantiJ. P. Vacanti Department of Surgery, Children's Hospital and Harvard Medical School, Boston, MA 02115More by J. P. Vacanti, and R. LangerR. Langer Department of Chemical Engineering, Massachusetts Institute of Technology, Boston, MA 02139More by R. LangerDOI: 10.1021/bk-1994-0540.ch002Publication Date (Print):November 30, 1993Publication History Published online23 July 2009Published inprint 30 November 1993Request reuse permissions Copyright © 1994 American Chemical Society. Polymers of Biological and Biomedical SignificanceChapter 2pp 16-34ACS Symposium SeriesVol. 540ISBN13: 9780841227323eISBN: 9780841213968Chapter Views103Citations10LEARN ABOUT THESE METRICSChapter Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Anatomy,Chemical engineering and industrial chemistry,Polymers,Regenerative medicine Get e-Alerts
Gastric Cancer Originating from Bone Marrow-Derived Cells (2004)

Epithelial cancers are believed to originate from transformation of tissue stem cells. However, bone marrow-derived cells (BMDCs), which are frequently recruited to sites of tissue injury and inflammation, might also represent a potential source of malignancy. We show that although acute injury, acute inflammation, or transient parietal cell loss within the stomach do not lead to BMDC recruitment, chronic infection of C57BL/6 mice with Helicobacter, a known carcinogen, induces repopulation of the stomach with BMDCs. Subsequently, these cells progress through metaplasia and dysplasia to intraepithelial cancer. These findings suggest that epithelial cancers can originate from marrow-derived sources and thus have broad implications for the multistep model of cancer progression.
Photodynamic therapy with Pd‐bacteriopheophorbide (TOOKAD): Successful in vivo treatment of human prostatic small cell carcinoma xenografts (2003)

Small cell carcinoma of the prostate (SCCP), although relatively rare, is the most aggressive variant of prostate cancer, currently with no successful treatment. It was therefore tempting to evaluate the response of this violent malignancy and its bone lesions to Pd-Bacteriopheophorbide (TOOKAD)-based photodynamic therapy (PDT), already proven by us to efficiently eradicate other aggressive non-epithelial solid tumors. TOOKAD is a novel bacteriochlorophyll-derived, second-generation photosensitizer recently, developed by us for the treatment of bulky tumors. This photosensitizer is endowed with strong light absorbance (epsilon(0) approximately 10(5) mol(-1) cm(-1)) in the near infrared region (lambda=763nm), allowing deep tissue penetration. The TOOKAD-PDT protocol targets the tumor vasculature leading to inflammation, hypoxia, necrosis and tumor eradication. The sensitizer clears rapidly from the circulation within a few hours and does not accumulate in tissues, which is compatible with the treatment of localized tumor and isolated metastases. Briefly, male CD1-nude mice were grafted with the human SCCP (WISH-PC2) in 3 relevant anatomic locations: subcutaneous (representing tumor mass), intraosseous (representing bone metastases) and orthotopically within the murine prostate microenvironment. The PDT protocol consisted of i.v. administration of TOOKAD (4 mg/kg), followed by immediate illumination (650-800 nm) from a xenon light source or a diode laser emitting at 770 nm. Controls included untreated animals or animals treated with light or TOOKAD alone. Tumor volume, human plasma chromogranin A levels, animal well being and survival were used as end points. In addition, histopathology and immunohistochemistry were used to define the tumor response. Subcutaneous tumors exhibited complete healing within 28-40 days, reaching an overall long-term cure rate of 69%, followed for 90 days after PDT. Intratibial WISH-PC2 lesions responded with complete tumor elimination in 50% of the treated mice at 70-90 days after PDT as documented histologically. The response of the orthotopic model was also analyzed histologically with similar results. The study with this model suggests that TOOKAD-based PDT can reach large tumors and is a feasible, efficient and well-tolerated approach for minimally invasive treatment of local and disseminated SCCP.
A Point-of-care Assay to Measure Platelet Aggregation in Patients Taking Clopidogrel (2006)

Clopidogrel is an important component of medical therapy for patients with acute coronary syndromes and those receiving coronary stents. Despite the use of clopidogrel, a significant number of patients experience recurrent adverse ischemic events. Inter-individual variability of platelet aggregation in response to clopidogrel may provide an explanation for some of these recurrent events, and small trials have linked clopidogrel resistance, as measured by platelet function tests, to adverse events. The VerifyNow P2Y12 assay (Accumetrics, Inc., CA, USA) is a point-of-care device that can accurately and rapidly measure the degree of platelet inhibition in patients taking clopidogrel. This assay can identify patients with a poor response to clopidogrel, and could potentially lead to change in therapy.
Determination of acid diffusion in chemical amplification positive deep ultraviolet resists (1991)

A new method was developed to study the diffusion of photogenerated acid in chemical amplification resist systems which allowed an estimation of the diffusion range by simple means. The acid mobility was investigated for two different resist systems under various process conditions. It was found that solvent traces in the film cause a very strong increase of the acid mobility. In order to control the diffusion range, the post-exposure-bake temperature must be below the glass transition temperature. For one resist system, the increase in resist sensitivity with increasing baking temperature was much smaller than the corresponding increase in diffusion range. The results corresponded well with those obtained by lithography with the same resist.
Soluble T-Cell Receptors Produced in Human Cells for Targeted Delivery (2015)

Recently, technology has become available to generate soluble T-cell receptors (sTCRs) that contain the antigen recognition part. In contrast to antibodies, sTCRs recognize intracellular in addition to extracellular epitopes, potentially increasing the number of applications as reagents for target detection and immunotherapy. Moreover, recent data show that they can be used for identification of their natural peptide ligands in disease. Here we describe a new and simplified expression method for sTCRs in human cells and show that these sTCRs can be used for antigen-specific labeling and elimination of human target cells. Four different TCRs were solubilized by expression of constructs encoding the TCR alpha (α) and beta (β) chains lacking the transmembrane and intracellular domains, linked by a ribosomal skipping 2A sequence that facilitates equimolar production of the chains. Cell supernatants containing sTCRs labeled target cells directly in a peptide (p)-human leukocyte antigen (HLA)-specific manner. We demonstrated that a MART-1p/HLA-A*02:01-specific sTCR fused to a fluorescent protein, or multimerized onto magnetic nanoparticles, could be internalized. Moreover, we showed that this sTCR and two sTCRs recognizing CD20p/HLA-A*02:01 could mediate selective elimination of target cells expressing the relevant pHLA complex when tetramerized to streptavidin-conjugated toxin, demonstrating the potential for specific delivery of cargo. This simple and efficient method can be utilized to generate a wide range of minimally modified sTCRs from the naturally occurring TCR repertoire for antigen-specific detection and targeting.
The use of peptide mimetics and proton magnetic resonance to define actin-binding sites on the myosin head (1990)

Conference Article| April 01 1990 The use of peptide mimetics and proton magnetic resonance to define actin-binding sites on the myosin head ANITA M. KEANE; ANITA M. KEANE 1School of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, U.K. Search for other works by this author on: This Site PubMed Google Scholar K. JOHN SMITH; K. JOHN SMITH 1School of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, U.K. Search for other works by this author on: This Site PubMed Google Scholar IAN P. TRAYER IAN P. TRAYER 1School of Biochemistry, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1990) 18 (2): 264–266. https://doi.org/10.1042/bst0180264a Article history Received: September 08 1989 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation ANITA M. KEANE, K. JOHN SMITH, IAN P. TRAYER; The use of peptide mimetics and proton magnetic resonance to define actin-binding sites on the myosin head. Biochem Soc Trans 1 April 1990; 18 (2): 264–266. doi: https://doi.org/10.1042/bst0180264a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1990 Biochemical Society1990 Article PDF first page preview Close Modal You do not currently have access to this content.
Intermediates in the Paal-Knorr Synthesis of Furans (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntermediates in the Paal-Knorr Synthesis of FuransVenkataraman Amarnath and Kalyani AmarnathCite this: J. Org. Chem. 1995, 60, 2, 301–307Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/jo00107a006https://doi.org/10.1021/jo00107a006research-articleACS PublicationsRequest reuse permissionsArticle Views3001Altmetric-Citations106LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Apolipoprotein L6, a Novel Proapoptotic Bcl-2 Homology 3–Only Protein, Induces Mitochondria-Mediated Apoptosis in Cancer Cells (2005)

Cancer cells frequently possess defects in the genetic and biochemical pathways of apoptosis. Members of the Bcl-2 family play pivotal roles in regulating apoptosis and possess at least one of four Bcl-2 homology (BH) domains, designated BH1 to BH4. The BH3 domain is the only one conserved in proapoptotic BH3-only proteins and plays an important role in protein-protein interactions in apoptosis by regulating homodimerization and heterodimerization of the Bcl-2 family members. To date, 10 BH3-only proapoptotic proteins have been identified and characterized in the human genome. The completion of the Human Genome Project and the availability of various public databases and sequence analysis algorithms allowed us to use the bioinformatic database-mining approach to identify one novel BH3-only protein, apolipoprotein L6 (ApoL6). The full-length cDNA of ApoL6 was identified, cloned, and functionally expressed in p53-null colorectal cancer cells (DLD-1). We found that overexpression of wild-type ApoL6 induced mitochondria-mediated apoptosis in DLD-1 cells characterized by release of cytochrome c and Smac/DIABLO from mitochondria and activation of caspase-9, whereas ApoL6 BH3 domain deletion allele did not. In addition, overexpression of ApoL6 also induced activation of caspase-8. Furthermore, we showed that adenovirus harboring the full-length cDNA of ApoL6 induced marked apoptosis in a variety of cancer cell types, and ApoL6 recruited and interacted with lipid/fatty acid components during the induction of apoptosis. To our knowledge, this is the first example that intracellular overproduction of an apolipoprotein induces marked apoptosis.
Host microenvironment in breast cancer development: Inflammatory cells, cytokines and chemokines in breast cancer progression: reciprocal tumor–microenvironment interactions (2002)

A comprehensive overview of breast cancer development and progression suggests that the process is influenced by intrinsic properties of the tumor cells, as well as by microenvironmental factors. Indeed, in breast carcinoma, an intensive interplay exists between the tumor cells on one hand, and inflammatory cells/cytokines/chemokines on the other. The purpose of the present review is to outline the reciprocal interactions that exist between these different elements, and to shed light on their potential involvement in breast cancer development and progression.
Supernatant from stored red blood cell primes inflammatory cells: influence of prestorage white cell reduction (2004)

Supernatant from stored S-RBCs prime unstimulated allogeneic PMNs in vitro. Prestorage buffy-coat WBC reduction was as effective as WBC depletion in abrogating this proinflammatory response elicited by supernatants from RBCs. The clinical consequences, if any, of these findings for transfusion recipients are unknown.
3,5-Diphenylpent-2-enoic Acids as Allosteric Activators of the Protein Kinase PDK1: Structure−Activity Relationships and Thermodynamic Characterization of Binding as Paradigms for PIF-Binding Pocket-Targeting Compounds†PDB code of 2Z with PDK1: 3HRF. (2009)

The modulation of protein kinase activities by low molecular weight compounds is a major goal of current pharmaceutical developments. In this line, important efforts are directed to the development of drugs targeting the conserved ATP binding site. However, there is very little experience on targeting allosteric, regulatory sites, different from the ATP binding site, in protein kinases. Here we describe the synthesis, cell-free activation potency, and calorimetric binding analysis of 3,5-diphenylpent-2-enoic acids and derivatives as allosteric modulators of the phosphoinositide-dependent kinase-1 (PDK1) catalytic activity. Our SAR results combined with thermodynamic binding analyses revealed both favorable binding enthalpy and entropy and confirmed the PIF-binding pocket of PDK1 as a druggable site. In conclusion, we defined the minimal structural requirements for compounds to bind to the PIF-binding pocket and to act as allosteric modulators and identified two new lead structures (12Z and 13Z) with predominating binding enthalpy.
A Computational Pipeline for High- Throughput Discovery of cis-Regulatory Noncoding RNA in Prokaryotes (2007)

Noncoding RNAs (ncRNAs) are important functional RNAs that do not code for proteins. We present a highly efficient computational pipeline for discovering cis-regulatory ncRNA motifs de novo. The pipeline differs from previous methods in that it is structure-oriented, does not require a multiple-sequence alignment as input, and is capable of detecting RNA motifs with low sequence conservation. We also integrate RNA motif prediction with RNA homolog search, which improves the quality of the RNA motifs significantly. Here, we report the results of applying this pipeline to Firmicute bacteria. Our top-ranking motifs include most known Firmicute elements found in the RNA family database (Rfam). Comparing our motif models with Rfam's hand-curated motif models, we achieve high accuracy in both membership prediction and base-pair-level secondary structure prediction (at least 75% average sensitivity and specificity on both tasks). Of the ncRNA candidates not in Rfam, we find compelling evidence that some of them are functional, and analyze several potential ribosomal protein leaders in depth.
Systematic screening of an arrayed cDNA library by PCR. (1995)

We have developed a PCR-based method for rapid and effective screening of arrayed cDNA libraries. This strategy directly addresses the limitations of conventional hybridization-based schemes and provides a more rapid, cost-effective, and sensitive method compatible with large-scale and routine cDNA clone recovery. To prepare arrayed libraries, 1-2 x 10(6) cDNA clones were propagated as individual plaques on solid medium in 24-well culture dishes at approximately 250 plaque-forming units per well. Phage suspensions were prepared from each well and transferred to a 96-well format. To screen the library, pools were generated that correspond to each individual 96-well plate and to each row and column within "blocks" of six plates each. Library screening for specific cDNA clones was conducted in a systematic and hierarchical fashion beginning with the plate pools. Next, the row/column pools corresponding to each positive plate pool were screened. Finally, isolated clones from within each positive well were identified by hybridization. We have applied this approach to the screening of an arrayed human brain cDNA library resulting in the recovery of cDNAs corresponding to > 25 genes and expressed sequence tags.
Whole blood treated with riboflavin and ultraviolet light: quality assessment of all blood components produced by the buffy coat method (2014)

Treatment of WB with riboflavin and UV before production of components by the BC method shows a negative impact on all three blood components. PLT units produced from PI-treated WB exhibited less damage compared to PLT component treatment.
Regulation of Complement C3 Expression by the Bile Acid Receptor FXR (2004)

The farnesoid X receptor (FXR; NR1H4) is an intracellular bile acid-sensing transcription factor that plays a critical role in the regulation of synthesis and transport of bile acids as well as lipid metabolism. Although the reciprocal relationship between bile acid and triglyceride levels is well known, the mechanism underlying this link is not clearly defined. In this study, we demonstrate that FXR regulates the expression of at least two secreted factors, complement component C3 and FGF15, the rat ortholog of FGF19, known to influence lipid metabolism. The analysis of the human complement C3 gene reveals the presence of functional FXR response elements in the proximal promoter of C3. Furthermore, rats given a single dose of an FXR agonist exhibit an increase in the plasma concentration of complement C3 protein. These studies demonstrate a mechanism by which FXR, a nuclear receptor with a limited tissue expression pattern, regulates secretion of factors that ultimately can affect lipid metabolism in an endocrine or paracrine manner.
A probabilistic model for data cube compression and query approximation (2007)

Databases and data warehouses contain an overwhelming volume of information that users must wade through in order to extract valuable and actionable knowledge to supportthe decision-making process. This contribution addresses the problem of automatically analyzing large multidimensional tables to get a concise representation of data, identify patterns and provide approximate answers to queries.