gebhart/patrepeval huggingface dataset:
p53 in chronic myeloid leukemia cell lines. (1992)

Four human chronic myeloid leukemia (CML) cell lines, BV173, K562, KCL-22, and KYO-1, were studied for inactivation of human tumor suppressor gene p53. Southern blotting showed allele deletion in KCL-22 and cytogenetic studies showed a chromosome 17 deletion in KYO-1 but no gross structural abnormalities in the other two lines. Northern blotting showed increased amounts of normal size p53 mRNA in BV-173 and KYO-1, trace amounts in KCL-22, and none in K562. Direct sequencing of p53 cDNA revealed a missense point mutation in KYO-1 and a single base pair deletion consistent with a coding frame shift in KCL-22. Both abnormalities in these myeloid cell lines were located in the highly conserved region of p53. Studies with two monoclonal antibodies showed that the three cell lines with p53 mRNA had readily detectable p53 proteins. In KYO-1 and BV173 cells the p53 protein was located mainly in the nuclei but KCL-22 cells had weak staining in the cytoplasm. Our data support the assumption that inactivation of p53 tumor suppressor function in myeloid blast transformation of CML may result from point mutations or deletions that produce mutant proteins.
Accuracy of Ultrasound-Guided Superficial Trigeminal Nerve Blocks Using Methylene Blue in Cadavers (2012)

This cadaveric investigation suggests that ultrasound guidance can be used to inject the peripheral trigeminal nerve with a high degree of accuracy. Clinicians should consider using ultrasound guidance to inject the trigeminal nerve for diagnostic or therapeutic purposes.
Quantitative Analysis of ZO-1 Co-Localization with Cx43 Gap Junction Plaques in Cultures of Rat Neonatal Cardiomyocytes (2004)

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.
A standard for business architecture description (1999)

A complete architectural specification of an information technology (IT) system includes information about how it is partitioned and how the parts are interrelated. It also contains information about what it should do and the purpose it must serve in the business. This paper provides a set of business concepts that partition the world of business meaning. It discusses the purpose of such an architectural view of business and ways in which it can be used. A set of generic concepts and their interrelationships organize business information content in terms of requirements on the business, the boundary of the business, and the business as a system for delivery of value. Methods are introduced to explore variations on the basic business concept patterns. These concepts are positioned to describe IT systems that support the business, and they are used to manage the work of IT system development and deployment.
Studies of Clostridium perfringens enterotoxin action at different temperatures demonstrate a correlation between complex formation and cytotoxicity (1990)

The cytotoxicity of Clostridium perfringens enterotoxin (CPE) was completely blocked in Vero cells continuously CPE treated at 4 degrees C. [125I]CPE-specific binding to either Vero cells or isolated rabbit intestinal brush border membranes (BBMs) was lower at 4 degrees C than at 24 or 37 degrees C, but reduced enterotoxin binding could not totally explain the loss of cytotoxicity at low temperature. Insertion of enterotoxin into Vero cell membranes or BBMs was temperature independent. However, CPE complex formation (A. P. Wnek and B. A. McClane, Infect. Immun. 57:574-581, 1989) in BBMs and Vero cells was blocked at 4 degrees C. When Vero cells were CPE treated at 4 degrees C, washed to remove unbound toxin, and then shifted to 37 degrees C, complex formation and cytotoxicity were rapidly detected. When CPE binding and complex formation were permitted for 2 min at 37 degrees C, and the Vero cells were then shifted to 4 degrees C, cytotoxicity was detectable at 4 degrees C. These results are consistent with complex formation, rather than complex activity, being the temperature-sensitive step in CPE action which is blocked at 4 degrees C. These studies demonstrate a strong correlation between complex formation and cytotoxicity and are consistent with complex involvement in CPE cytotoxicity. These studies also strongly suggest that CPE insertion precedes both complex formation and induction of small-molecule permeability changes.
Design and Characterization of a Heterodimeric Coiled Coil that Forms Exclusively with an Antiparallel Relative Helix Orientation (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDesign and Characterization of a Heterodimeric Coiled Coil that Forms Exclusively with an Antiparallel Relative Helix OrientationDiana L. McClain, Howard L. Woods, and Martha G. OakleyView Author Information Department of Chemistry, Indiana University 800 East Kirkwood Avenue, Bloomington, Indiana 47405-7102 Cite this: J. Am. Chem. Soc. 2001, 123, 13, 3151–3152Publication Date (Web):March 13, 2001Publication History Received28 November 2000Published online13 March 2001Published inissue 1 April 2001https://pubs.acs.org/doi/10.1021/ja004099lhttps://doi.org/10.1021/ja004099lrapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views1648Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Assays,Equilibrium,Mixtures,Peptides and proteins,Rearrangement Get e-Alerts
Spontaneous neuronal hyperactivity in the medial and intralaminar thalamic nuclei of patients with deafferentation pain (1991)

Electrical activity was recorded from single cells in the thalamus of 10 patients with chronic pain associated with deafferentation. Under local anesthesia, these patients underwent either electrode implantation or thalamotomy for treatment of their pain. In eight of the 10 patients, single units were identified as discharging spontaneously in high-frequency, often rhythmic, bursts. The discharges were of two types: short bursts comprised of two to six spikes with a burst frequency of one to four per second; and long trains of 30 to 80 spikes of similar frequency. Reconstruction of electrode trajectories indicated that recordings were made from the region corresponding to the lateral aspect of the mediodorsal thalamic nucleus, the central lateral nucleus, a small part of the central median nucleus, and the parafascicular nucleus. In the eight patients in whom spontaneous neuronal burst activity was exhibited, it was impossible to study activity evoked by natural cutaneous stimulation due to the continuous spontaneous neuronal discharges. Both animal and human studies have suggested that pain related to deafferentation is accompanied by spontaneous hyperactivity in the dorsal horn of the spinal cord and in the ventral posterior thalamic nuclei. The authors present evidence of spontaneous neuronal hyperactivity in the intralaminar thalamic nuclei of patients with pain related to deafferentation. The findings suggest that spontaneous neuronal discharge in patients with pain related to deafferentation is more widespread in the central nervous system than has been previously appreciated. The results have important implications for the surgical treatment of chronic pain.
A HW/SW co-designed heterogeneous multi-core virtual machine for energy-efficient general purpose computing (2011)

It is increasingly challenging to improve single thread performance because power/energy consumption becomes a major barrier to achieve significantly higher performance for general purpose cores. General purpose processors are designed to perform well in a wide variety of market segments, at the cost of having significantly lower performance-per-watt than special purpose processors targeting limited applications or market segments. In this paper, we propose a HW/SW co-designed heterogeneous multi-core virtual machine, called TwinPeaks, which integrates a set of less general but power efficient cores and uses dynamic binary optimization to schedule code regions to run on the most efficient cores. Our experiment and analysis indicate that TwinPeaks with a wide in-order core and a narrow out-of-order core may achieve 108% performance at ˜71% energy of a big 4-wide out-of-order core.
Cas9-Guide RNA Directed Genome Editing in Soybean (2015)

Recently discovered bacteria and archaea adaptive immune system consisting of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) endonuclease has been explored in targeted genome editing in different species. Streptococcus pyogenes Cas9-guide RNA (gRNA) was successfully applied to generate targeted mutagenesis, gene integration, and gene editing in soybean (Glycine max). Two genomic sites, DD20 and DD43 on chromosome 4, were mutagenized with frequencies of 59% and 76%, respectively. Sequencing randomly selected transgenic events confirmed that the genome modifications were specific to the Cas9-gRNA cleavage sites and consisted of small deletions or insertions. Targeted gene integrations through homology-directed recombination were detected by border-specific polymerase chain reaction analysis for both sites at callus stage, and one DD43 homology-directed recombination event was transmitted to T1 generation. T1 progenies of the integration event segregated according to Mendelian laws and clean homozygous T1 plants with the donor gene precisely inserted at the DD43 target site were obtained. The Cas9-gRNA system was also successfully applied to make a directed P178S mutation of acetolactate synthase1 gene through in planta gene editing.
Capsaicin evoked pain and allodynia in post-herpetic neuralgia (2000)

The hypothesis that the pain and allodynia associated with post-herpetic neuralgia (PHN) is maintained by a combination of input from preserved primary afferent nociceptors and sensitization of central pain transmitting neurons was examined in 17 subjects with PHN. Pain, allodynia, thermal sensory function, cutaneous innervation, and response to controlled application of 0.075% capsaicin were measured. Compared to mirror-image skin, applying capsaicin on a 9 cm(2) area of PHN skin significantly increased overall PHN pain and allodynia in 11 of 17 subjects. These 'capsaicin responders' were characterized by higher average daily pain, higher allodynia ratings, and relatively preserved sensory function at baseline compared to the non-responders. In three of the 'capsaicin responders' the area of allodynia expanded into previously non-allodynic and non-painful skin that had normal sensory function and cutaneous innervation. These observations support the hypothesis that allodynia in some PHN patients is a form of chronic secondary hyperalgesia maintained by input from intact and possibly 'irritable' primary afferent nociceptors to a sensitized CNS.
Improved diagnosis of classical vs atypical phenylketonuria by liquid chromatography. (1984)

Atypical phenylketonuria among infants with hyperphenylalaninemia must be promptly diagnosed and differentiated from classical phenylketonuria, because these patients require different treatment to prevent irreversible neurological damage. Measurement of pteridines in urine by liquid chromatography has been widely used for this purpose. Here we report a rapid, simplified liquid-chromatographic method for simultaneously measuring urinary biopterin, neopterin, and creatinine. Unlike previous methods, this method involves neither tedious cleanup procedures nor special equipment.
Heparanase and a Synthetic Peptide of Heparan Sulfate-interacting Protein Recognize Common Sites on Cell Surface and Extracellular Matrix Heparan Sulfate (1997)

Heparanase is an endo-beta-D-glucuronidase that degrades the glycosaminoglycan chains of heparan sulfate (HS) proteoglycans at specific sites. Elevated levels of heparanase are associated with the metastatic potential of melanoma and other types of tumor cells. We previously reported heparanase degradation of cell surface HS subpopulations of the human adenocarcinoma cell line RL95. In the present study, heparanase activity was examined on RL95 cell surface HS subpopulations in the presence of a synthetic peptide (CRPKAKAKAKAKDQTK) of heparin/heparan sulfate-interacting protein (HIP; Liu, S., Smith, S. E., Julian, J., Rohde, L. H., Karin, N. J., and Carson, D. D. (1996) J. Biol. Chem. 271, 11817-11823). Heparanase digestion generated HS fragments from cell surface- or extracellular matrix-derived HS of approximately 25 and 9 kDa, respectively. In contrast, HS of various size classes isolated from proteoglycans secreted or released by RL95 and endothelial cells in culture were not susceptible to heparanase digestion. Incubation of heparanase-containing melanoma cellular extracts or partially purified heparanase preparations with cell surface- or ECM-derived HS and HIP peptide, but not a scrambled sequence of this peptide or other HS-binding proteins present in ECM, completely inhibited heparanase action. Conversely, predigestion of cell surface HS with either heparanase-containing cellular extracts or with secreted or partially purified heparanase destroyed binding to HIP peptide. Preincubation of HS with HIP peptide prevented subsequent heparanase digestion. Collectively, these data demonstrate that HIP peptide and heparanase recognize specific, common motifs within HS chains at cell surfaces and in ECM and may mutually modulate HS-dependent activities.
Beta-carotene supplementation associated with intermittent retinol administration in the treatment of premenopausal mastodynia. (1989)

Twenty-five women, 23-41 year old, suffering from premestrual cyclical mastodynia linked or otherwise to benign breast disease (BBD), with moderate or severe pain at least seven days before each menstrual period, were treated with daily beta-carotene (BC) supplementation associated with intermittent administration of retinol (all-trans-retinol 300,000 IU per day). In this therapy retinol was given for 7 days immediately before each menstrual period. After 6 months' treatment, the results revealed marked reduction in breast pain, and sometime recovery, in 23-41 year old women with no toxic side effects. But no such advantages in 5 women with non-cyclical mastodynia treated as above were found. Above this age range, the advantages appear to be absent. All the women developed a healthy look because of a slight tanning of the skin due to beta-carotene supplementation. These data demonstrated a therapeutic synergism between BC and retinol.
Generation of 18-fs, multiterawatt pulses by regenerative pulse shaping and chirped-pulse amplification (1996)

Transform-limited, 18-fs pulses of 4.4-TW peak power are produced in a Ti:sapphire-based chirped-pulsed amplification system at a repetition rate of 50 Hz. Regenerative pulse shaping is used to control gain narrowing during amplification, and an optimized, quintic-phase-limited dispersion compensation scheme is used to control higher-order phase distortions over a bandwidth of ~100 nm. Seed pulses are temporally stretched >100,000 times before amplification.
The development of a sinusoidal voltage in thin ferromagnetic films and the measurement of low magnetic fields (1978)

Magnetoresistance measurements have been made in thin films using a special experimental set-up. Effects due to a rotating magnetic field applied in the sample plane have been studied when the sample is saturated and when it is unsaturated, and they have been used to measure low magnetic fields with the sample acting as a detector.
有机可焊性保护剂(OSP)的发展 (1998)

1 前言 OSP(Organic Solderability Pre-servatives)具有多种种类和型号。但它们的基本化学特性属于咪唑或三唑类化合物中的一种,但相似之处仅止于此。在过去若干年中,产品所经历的变化包括涂覆厚度变化、水对溶剂的溶解度以及咪唑和苯基咪唑的络合反应生成物等。所有OSP的关键功能性问题依然是保护基体金属钢在经过多个热循环之后的可焊性。另一个严肃问题是与OSP涂层的厚度有关。正如可焊性结果所表明的,较厚
Supplementation with β-Hydroxy- β-Methylbutyrate (HMB) and α-Ketoisocaproic Acid (KIC) Reduces Signs and Symptoms of Exercise-Induced Muscle Damage in Man (2005)

This study examined the effects of beta-hydroxyl-beta-methylbutyrate (HMB) and alpha-ketoisocaproic acid (KIC) supplementation on signs and symptoms of exercise-induced muscle damage following a single bout of eccentrically biased resistance exercise. Six non-resistance trained male subjects performed an exercise protocol designed to induce muscle damage on two separate occasions, performed on the dominant or non-dominant arm in a counter-balanced crossover design. Subjects were assigned to an HMB/KIC (3 g HMB and 0.3 g alpha-ketoisocaproic acid, daily) or placebo treatment for 14 d prior to exercise in the counter-balanced crossover design. One repetition maximum (1RM), plasma creatine kinase activity (CK), delayed onset muscle soreness (DOMS), limb girth, and range of motion (ROM) were determined pre-exercise, at 1h, 24 h, 48 h, and 72 h post-exercise. DOMS and the percentage changes in 1RM, limb girth, and ROM all changed over the 72 h period (P < 0.05). HMB//IC supplementation attenuated the CK response, the percentage decrement in 1RM, and the percentage increase in limb girth (P < 0.05). In addition, DOMS was reduced at 24 h post-exercise (P < 0.05) in the HMB/KIC treatment. In conclusion, 14 d of HMB and KIC supplementation reduced signs and symptoms of exercise-induced muscle damage in non-resistance trained males following a single bout of eccentrically biased resistance exercise.
BIBN4096BS Antagonizes Human α-calcitonin Gene Related Peptide–induced Headache and Extracerebral Artery Dilatation* (2005)

These results show that BIBN4096BS effectively prevents CGRP-induced headache and extracerebral vasodilatation but does not significantly affect the induced cerebral hemodynamic changes.
Leveraging ICN In-network Control for Loss Detection and Recovery in Wireless Mobile networks (2016)

One of the most appealing features of Information-Centric Networking (ICN) is its agile connectionless transport model based on consumer requests and hop-by-hop forwarding. By relaxing end-to-end constraints, ICN empowers a distributed in-network control with the potential to improve congestion management over heterogeneous wired/wireless media and in presence of mobility. However, little effort has been devoted so far to the exploration of ICN capabilities in this space. In this paper, we contribute an understanding of the opportunities for ICN in-network control over wireless mobile networks and a proposal for simple, yet very effective mechanisms for in-network loss detection and recovery to complement receiver-driven control. More precisely, we introduce (i) WLDR, a mechanism for in-network Wireless Loss Detection and Recovery that promptly identifies and recovers channel losses at wireless access point and (ii) MLDR, a mechanism for preventing losses due to consumer/producer mobility via explicit network notification and dynamic on-the-fly request re-routing. We setup a realistic wireless simulation environment in ndn-SIM using IEEE 802.11n connectivity and evaluate WLDR-MLDR performance. The results show significant benefits over consumer-based solutions with or without explicit loss notification, while also removing any dependency from network and application timers.
Experimental cancer cachexia: the role of host-derived cytokines interleukin (IL)-6, IL-12, interferon-gamma, and tumor necrosis factor alpha evaluated in gene knockout, tumor-bearing mice on C57 Bl background and eicosanoid-dependent cachexia. (2000)

MCG 101 tumors were implanted sc. on wild-type C57 Bl and gene knockout mice to evaluate the role of host-produced cytokines [interleukin (IL)-6, IL-12, IFNgamma, tumor necrosis factor (TNF) receptor 1, and TNF receptor 2] to explain local tumor growth, anorexia, and carcass weight loss in a well-defined model with experimental cachexia. Indomethacin was provided in the drinking water to explore interactions between host and tumor-derived prostaglandins and proinflammatory cytokines for tumor growth. Wild-type tumor-bearing mice developed cachexia because of rapid tumor growth, which were both attenuated in IL-6 gene knockouts. Similar findings were observed after provision of anti-IL-6 to wild-type tumor-bearing mice. Alterations in food intake were not directly related to systemic IL-6 but rather secondarily to IL-6-dependent tumor growth. The absence of host-derived IL-12, IFN-gamma, or the TNF receptor 1 or receptor 2 gene did not attenuate tumor growth or improve subsequent cachexia. Thus, carcass weight loss was not improved by the omission of host cytokine (TNF-alpha, IL-12, or IFN-gamma) except for IL-6. Systemic indomethacin provision decreased plasma prostaglandin E2 in five of six groups of gene knockout tumor-bearing mice, which was associated with improved carcass weight in these groups. Indomethacin seemed to improve food intake to a similar extent in both wild-type and gene knockouts, which agree with the speculation that eicosanoids are more important to explain anorexia than host cytokines. Our results demonstrate that host- and tumor-derived cytokines and prostaglandins interact with tumor growth and promote cachexia in a more complex fashion than usually presented based on previous information in studies on either anti-cytokine experiments in vivo or on gene knockouts with respect to a "single cytokine model." Overall, host cytokines were quantitatively less important than tumor-derived cytokines to explain net tumor growth, which indirectly explains subsequent cachexia and anorexia.
Novel Poly(butylene succinate)‐Based Ionomers with Sulfonated Succinate Units: Synthesis, Morphology, and the Unique Nucleation Effect on Crystallization (2005)

Abstract Summary: Poly(butylene succinate) (PBS) and PBS‐based ionomers (PBSi's) with 0.3 and 1.2 mol‐% of sodium sulfonated succinate unit were synthesized. The existence of ionic aggregates in the ionomer matrices was indicated by melt viscosity and glass transition data. It seems that the ionic aggregates have two opposing roles in crystallization: one is the induction of nucleation and the other is the interference with lamellar growth. Because of these two contrary effects, the crystallization behavior of the ionomers exhibited measurably different cooling rate‐dependencies than that of the parent PBS. The rate varied distinctly with ionic content. Spherulitic morphologies of PBS and PBSi's measured with polarizing optical microscopy. magnified image Spherulitic morphologies of PBS and PBSi's measured with polarizing optical microscopy.
A multi-microprocessor computer system architecture (1975)

The development of microprocessors has suggested the design of distributed processing and multiprocessing computer architectures. A computer system design incorporating these ideas is proposed, along with its impact on memory management and process control aspects of the system's operating system. The key design feature is to identify system processes with microprocessors and interconnect them in a hierarchy constructed to minimize intercommunication requirements.