gebhart/patrepeval huggingface dataset:
Second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes (1987)

A modified-live pseudorabies virus (PRV) vaccine, designated PRV(dlg92/d1tk), with deletions in the thymidine kinase (tk) and glycoprotein-gIII (g92) genes, was derived from the PRV (Bucharest [BUK]-d13) vaccine strain. The vaccine virus also contained a deletion in glycoprotein gI. Despite 3 deletions, PRV(dlg92/d1tk) replicated to high titers in cell culture from 30 C to 39.1 C. Enzyme assays and autoradiography revealed that PRV(dlg92/d1tk) did not induce a functional tk activity in infected tk- RAB(BU) cells (rabbit skin). Rabbit skin cells were infected with PRV(dlg92/d1tk), with vaccine strains derived from BUK or Bartha K strains of PRV or with the virulent Illinois (ILL), Indiana-Funkhauser (IND-F), and Aujeszky (Auj) strains of PRV and were labeled with [3H]mannose from 4 or 5 to 24 hours after infection to investigate whether these viruses induced the synthesis of glycoprotein gIII. Nonionic detergent extracts were prepared and immunoprecipitated with antisera from pigs vaccinated with tk(-)-PRV(BUK-d13) or tk+-Bartha K, pigs vaccinated with tk+-PRV(BUK) strains and then challenge exposed to tk+-PRV(IND-F), naturally infected domestic or feral pigs, and pigs vaccinated with tk-)-PRV(dlg92/d1tk). Mouse monoclonal antibodies against PRV glycoproteins gIII, gp50, and gII were also studied. After immunoprecipitation, labeled PRV-specific proteins were analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis and autoradiography. The PRV glycoprotein-gII complex, but not glycoprotein gIII, was synthesized in PRV(dlg92/d1tk)-infected cells. Glycoprotein gII and gIII were made in cells infected with PRV vaccine strains BUK, Bartha K, and BUK-d13 and with virulent PRV strains ILL, IND-F, and Auj. Cells infected with PRV(dlg92/d1tk) and with PRV strains ILL, IND-F, Auj, Bartha K, BUK, and BUK-d13, excreted into the cell culture medium a highly sulfated glycoprotein gX of about 90 kilodaltons. Antibodies to glycoprotein gIII were not detected in the sera of pigs inoculated with PRV(dlg92/d1tk), but were found in all other swine sera.
Chrysophanol, an Antimicrobial Anthraquinone from the Root Extract of Colubrina greggii (2020)

Abstract. The methanolic extract of the root of Colubrina greggii S. Watson showed antimicrobial activity against Bacillus subtilis andStaphylococcus aureus. The bioassay-guided purification of the organic crude extract resulted in the isolation and identification ofchrysophanol (1,8-dihydroxy-3-methylanthracenedione), as the metabolite responsible for the antimicrobial activity.Resumen. El extracto metanólico de las raíces de Colubrina greggii S. Watson mostró actividad antibacteriana contra Bacillus subtilis y Staphylococcus aureus. La purificación biodirigida del extracto orgánico crudo de las raíces permitió el aislamiento y la identificación de crisofanol (1,8-dihydroxy-3-methylanthracenediona) como el metabolito responsable de la actividad antimicrobiana.
Endotoxin-induced uveitis in the rat is attenuated by inhibition of nitric oxide production. (1994)

These results suggest that the hemodynamic and vascular permeability changes associated with endotoxin-induced uveitis are mediated in large part by increased production of nitric oxide.
Minocycline Treatment Reduces Delayed Oligodendrocyte Death, Attenuates Axonal Dieback, and Improves Functional Outcome after Spinal Cord Injury (2004)

Minocycline has been demonstrated to be neuroprotective after spinal cord injury (SCI). However, the cellular consequences of minocycline treatment on the secondary injury response are poorly understood. We examined the ability of minocycline to reduce oligodendrocyte apoptosis, microglial/macrophage activation, corticospinal tract (CST) dieback, and lesion size and to improve functional outcome after SCI. Adult rats were subjected to a C7-C8 dorsal column transection, and the presence of apoptotic oligodendrocytes was assessed within the ascending sensory tract (AST) and descending CST in segments (3-7 mm) both proximal and distal to the injury site. Surprisingly, the numbers of dying oligodendrocytes in the proximal and distal segments were comparable, suggesting more than the lack of axon-cell body contiguity played a role in their demise. Minocycline or vehicle control was injected into the intraperitoneal cavity 30 min and 8 hr after SCI and thereafter twice daily for 2 d. We report a reduction of apoptotic oligodendrocytes and microglia within both proximal and distal segments of the AST after minocycline treatment, using immunostaining for active caspase-3 and Hoechst 33258 staining in combination with cell-specific markers. Activated microglial/macrophage density was reduced remote to the lesion as well as at the lesion site. Both CST dieback and lesion size were diminished after minocycline treatment. Footprint analysis revealed improved functional outcome after minocycline treatment. Thus, minocycline ameliorates multiple secondary events after SCI, rendering this clinically used drug an attractive candidate for SCI treatment trials.
Inhibition of a store‐operated Ca2+ entry pathway in human endothelial cells by the isoquinoline derivative LOE 908 (1996)

1. The novel cation channel blocker, LOE 908, was tested for its effects on Ca2+ entry and membrane currents activated by depletion of intracellular Ca2+ stores in human endothelial cells. 2. LOE 908 inhibited store-operated Ca2+ entry induced by direct depletion of Ca2+ stores with 100 nM thapsigargin or 100 nM ionomycin with an EC50 of 2 microM and 4 microM, respectively. 3. LOE 908 did not affect thapsigargin- or ionomycin-induced Ca2+ release from intracellular stores up to concentrations of 3 microM. 4. LOE 908 reversibly suppressed thapsigargin- as well as ionomycin-induced whole-cell membrane currents. 5. The LOE 908-sensitive membrane conductance corresponded to a cation permeability of 5.5 and 6.9 fold selectivity for Ca2+ over K+ in the presence of thapsigargin and ionomycin, respectively. 6. Our results suggest that the isoquinoline, LOE 908 is a novel, potent inhibitor of the store-operated (capacitive) Ca2+ entry pathway in endothelial cells.
Novel technologies and therapeutic use (2004)

Volume 1:- 1. Concepts in Development of Manufacturing Strategies for Monoclonal Antibodies B. Fish. 2. Antibody Fragments: Production, Purification and Formatting for Therapeutic Applications D.J. Glover, D.P. Humphreys. 3. Large Scale Production of Therapeutic Antibodies: Considerations for Optimizing Product Capture and Purification G. Kemp, P. O'Neil. 4. Scale-Up of Antibody Purification. From Laboratory Scale to Production L.R. Jacob, M. Frech. 5. Purification of Antibody by Chromatographic Methods C. Vandevyver, R. Freitag. 6. Quality Control of Antibodies for Human Use A. Richter, M. Jostameling, K. Muller, A. Herrmann, M. Pitschke. 7. Expression of Human Anti Rh(D) Monoclonal Antibodies into Different Cell lines: Influence on their Functional Properties C. de Romeuf, C. Gaucher, A. Glacet, S. Jorieux, P. Klein, D. Bourel. 8. Monoclonal Antibody Production: Minimising Virus Safety Issues J. Bray, M.K. Brattle. 9. Contract Manufacturing of Biopharmaceutical Grade Antibodies L.A. Van Der Pol, D.F. Westra. 10. The Pharmacokinetics and Pharmacodynamics of Monoclonal Antibodies D.B. Haughey, P.M. Jardieu. 11. Regulatory and Legal Requirements for the Manufacture of Antibodies L. Tsang. Index. Volume 2:- 1. Transgenic technology for monoclonal antibody production S. Sensoli. 2. Generation, engineering and production of human antibodies using HuCAL R. Ostendorp, C. Frisch, M. Urban. 3. Achieving appropriate glycosylation during the scaleup of antibody production X.K. Deng, T. Shantha Raju, K.J. Morrow Jr. 4. Approaches to devise antibody purification processes by chromatography E. Boschetti. 5. Novel HIV neutralizing antibodies selected from phage display libraries M. Moulard, Mei-Yun Zhang, D.S. Dimitrov. 6. Monoclonal and bispecific antibodies in combination with radiotherapy for cancer treatment D. Azria, C. Larbouret, B. Robert, M. Ozsahin, J.B. Dubois, A. Pelegrin. 7. Antibodies, a potent tool to target genes into designated cells and tissues F. Hirsch, O. Deas, G. Carvalho, A. Durrbach, D. Thierry, A. Chapel. 8. Intrabodies: development and application in functional genomics and therapy A. Barberis, A. Auf Der Maur, K. Tissot, P. Lichtlen. 9. Expression of recombinant antibodies by tumour cells: on road to anti-tumour therapy E. Bonnin, J.-L. Teillaud. 10. Antitumor antibodies: from research to clinic S. Menard, S.M. Pupa, M. Campiglio, E. Tagliabue. 11. Macrophages for immunotherapy D. Loirat, S. Jacod, A. Boyer, F. Auzelle, A. McAllister, J.-P. Abstado, J. Bartholeyns, D. Prigent. 12. Future prospects in antibody engineering and therapy S. Siberil, J.-L. Teillaud. Index.
Molecular biotechnology: Principles and applications pf recombinant DNA (2010)

Molecular biotechnology: Principles and applications pf recombinant DNA , Molecular biotechnology: Principles and applications pf recombinant DNA , کتابخانه دانشگاه علوم پزشکی و خدمات بهداشتی درمانی کرمان
Position-Dependent Processing of Peptides Presented on the Surface of Cowpea Mosaic Virus (1999)

The plant virus cowpea mosaic virus (CPMV) has been developed as an epitope-presentation system. Numerous epitopes have been expressed in the betaB-betaC loop of the CPMV small coat protein, all of which undergo a cleavage reaction between their two carboxy-terminal residues. Although many peptides presented in this manner give an authentic immune response, this was not the case for the NIm-1A epitope from human rhinovirus-14. Crystallography revealed significant differences between the structure of NIm-1A on CPMV compared with its native configuration. The 3D structure of C PMV expressing NIm-1A was used to design alterations to the context of the NIm-1A graft.
The Acute Effects of Human Growth Hormone Administration on Thyroid Function in Normal Men* (1988)

GH replacement therapy may lead to alterations in serum TSH and/or thyroid hormone values in GH-deficient patients, but there is no consensus on the explanation for these changes. We examined the effect of GH administration (0.125 mg, sc, daily for 4 days) on thyroid function in 20 normal men. Serum T4 levels decreased by 8%, and serum free T4 index values decreased by 5%. In contrast, serum T3 levels increased by 21%; serum rT3 did not change. These changes were accompanied by a 54% decrease in the mean serum TSH level. While it is not possible to draw conclusions about hormone production and disposal rates from changes in serum levels, these data are most consistent with enhanced extrathyroidal (including intrapituitary) conversion of T4 to T3 and a compensatory decrease in TSH secretion.
A New Workflow For Petrophysical And Textural Evaluation of Carbonate Reservoirs (2008)

Several researchers have noted the association between rock texture and petrophysical properties in carbonates. Accurate petrophysical evaluation requires the integration of texture-sensitive logs such as nuclear magnetic resonance, borehole images, full waveform acoustics, and dielectric. Lithology and porosity evaluation in the presence of anhydrite or other minerals may require the use of neutron capture spectroscopy logs. A comprehensive, accurate petrophysical evaluation of carbonates requires a well-engineered workflow to enable rapid integration of the data from this large suite of logs. We describe an interpretation methodology and workflow that facilitates the integration of log measurements essential to accurate carbonate evaluation. The workflow is the result of decades of research into carbonate petrophysics. The paper shows that the method provides a robust and comprehensive petrophysical description of lithology, porosity, tar volume, pore geometry, permeability, petrophysical rock types, fluid saturations, relative permeabilities, and primary drainage capillary pressure curves. Examples are presented from major Middle East carbonate reservoirs. We also present a software implementation that leverages the methodology through an intuitive and user-friendly workflow. The method has been applied on several wells in the Middle East in near real time thereby permitting decisions on subsequent data acquisition and on the completion of the well.
Deterioration of kidney function by the (pro)renin receptor blocker handle region peptide in aliskiren-treated diabetic transgenic (mRen2)27 rats (2014)

Dual renin-angiotensin system (RAS) blockade in diabetic nephropathy is no longer feasible because of the profit/side effect imbalance. (Pro)renin receptor [(P)RR] blockade with handle region peptide (HRP) has been reported to exert beneficial effects in various diabetic models in a RAS-independent manner. To what degree (P)RR blockade adds benefits on top of RAS blockade is still unknown. In the present study, we treated diabetic TGR(mREN2)27 rats, a well-established nephropathy model with high prorenin levels [allowing continuous (P)RR stimulation in vivo], with HRP on top of renin inhibition with aliskiren. Aliskiren alone lowered blood pressure and exerted renoprotective effects, as evidenced by reduced glomerulosclerosis, diuresis, proteinuria, albuminuria, and urinary aldosterone levels as well as diminished renal (P)RR and ANG II type 1 receptor expression. It also suppressed plasma and tissue RAS activity and suppressed cardiac atrial natriuretic peptide and brain natriuretic peptide expression. HRP, when given on top of aliskiren, did not alter the effects of renin inhibition on blood pressure, RAS activity, or aldosterone. However, it counteracted the beneficial effects of aliskiren in the kidney, induced hyperkalemia, and increased plasma plasminogen activator-inhibitor 1, renal cyclooxygenase-2, and cardiac collagen content. All these effects have been linked to (P)RR stimulation, suggesting that HRP might, in fact, act as a partial agonist. Therefore, the use of HRP on top of RAS blockade in diabetic nephropathy is not advisable.
SPARC and CA19–9 as biomarkers in patients with advanced pancreatic cancer treated with nab paclitaxel plus gemcitabine (2008)

15592 Background: Pancreatic cancers are known to overexpress SPARC (Secreted Protein And Rich in Cysteine) (Von Hoff et al., 2006). Increased SPARC expression in tumors resulted in improved response to albumin- bound paclitaxel (nab-paclitaxel, nab-pac) due to SPARC-albumin binding (Trieu et al., SKCC, 2007). A disease specific phase I trial of nab-pac + gemcitabine (gem) in pts with advanced pancreatic cancer was initiated and pretreatment tumor biopsies tested for SPARC to investigate any correlation with response. Serum CA19–9 levels were monitored since a decrease in this biomarker was correlated with improved survival by multiple investigators (Ko et al., 2005, Maisey et al 2005). Methods: Pts with no prior chemotherapy except as a radiation sensitizer received nab-pac (100 mg/m2) + gem (1000 mg/m2) on days 1, 8, 15 every 28 days. Response (RECIST) was determined by CAT or PET scans. SPARC immunohistochemistry (IHC) in panc tumors was scored on a 0–3 scale (3=positive) at a central laboratory. CA19–9 was monitored at every cycle. Fisher's exact test was used for statistical analysis. Results: To date, 20 pts are enrolled. 16 pts were evaluable for response (9PR [5 confirmed, 4 unconfirmed], 6SD, 1PD). Clinical details are reported in another abstract. CA19–9 data were available for 15 evaluable patients. CA19–9 decrease of ≥40% from baseline was noted in 11/15 (73%) pts. CA19–9 drop of ≥40% correlated with response and occurred in all 9 responders and only in 2 of 6 non-responders (p = 0.011). SPARC IHC was available for 12 evaluable pts. SPARC positive pts (8/12) included 6PR, 2SD; while SPARC negative pts included 1PR, 2SD and 1PD. Patients with response were more likely to be SPARC positive. Conclusions: The combination of nab-paclitaxel and gemcitabine appears to be active in pancreatic cancer and warrants further study. These preliminary data suggest that SPARC positivity and decrease in CA19–9 may correlate with response and may support the hypothesis that SPARC plays an important role in the concentration of albumin bound paclitaxel in tumors. The study has been expanded to enroll additional pts. Author Disclosure Employment or Leadership Consultant or Advisory Role Stock Ownership Honoraria Research Expert Testimony Other Remuneration Abraxis BioScience, LLC Concordia, Genentech Abraxis BioScience, LLC Abraxis BioScience, LLC, Anza, Bristol-Myers Squibb, Cell Genesys, Concordia, Genentech, Genvec
Vascular endothelial growth factor expression in primary esophageal squamous cell carcinoma (1997)

The results of this study suggest that VEGF expression is associated with tumor progression and poor prognosis by stimulating angiogenesis in esophageal squamous cell carcinoma.
Reversible binding of dioxygen to mesoporphyrin IX derivatives at low temperatures (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReversible binding of dioxygen to mesoporphyrin IX derivatives at low temperaturesJoseph Almog, Jack E. Baldwin, Robert L. Dyer, Joel Huff, and Carlos J. WilkersonCite this: J. Am. Chem. Soc. 1974, 96, 17, 5600–5601Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://doi.org/10.1021/ja00824a063RIGHTS & PERMISSIONSArticle Views98Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (243 KB) Get e-Alertsclose Get e-Alerts
Laminin facilitates and guides fiber growth of transplanted neurons in adult brain. (1989)

Laminin has been shown in vitro to act as a surface adhesive molecule for neuronal process elongation. To test whether laminin has a similar role in the brain, we sequentially injected laminin and transplanted fetal neurons into various brain regions to determine if the fetal neurons would preferentially grow along a laminin injection tract. In the fetal brain, the raphe area of the rostral rhombencephalon is rich in serotonergic (5-HT) neurons; the rostral ventral mesencephalon is rich in dopamine (DA) neurons, while the lateral rhombencephalon is rich in norepinephrinergic (NE) neurons. These three areas were transplanted to the motor cortex, neostriatum or hippocampus of adult animals. The tract used for microinjection of cell suspension was then immediately filled with laminin in a suspension media or a laminin-collagen (type IV) mixture. In other animals, laminin or control solution was injected in a separate needle tract displaced 0.3-1 mm from the transplant injection tract. Straight and thick 5-HT, DA or NE immunoreactive (IR) fibers (stained with anti-5-HT or anti-tyrosine hydroxylase antiserum) were predominant within the laminin-treated tracts, or were directed toward the laminin-treated parallel tracts when it was positioned less than 0.5 mm from the transplant site. The density of 5-HT-, DA- and NE-IR fibers in the injection tracts in all three brain areas was much higher for laminin and laminin-collagen mixture than control media. Thin axonal fibers of fetal 5-HT and NE neurons were observed surrounding the laminin-treated tracts, but not around vehicle-injected tracts. In addition, a number of transplanted 5-HT, DA and NE neuronal cell bodies were seen within the laminin-treated tracts, but not in vehicle-treated tracts. Finally, laminin injection to the hippocampus, motor cortex or neostriatum of the adult brain did not stimulate sprouting of undamaged adult 5-HT or NE fibers. These results suggest that purified laminin can facilitate and guide process outgrowth of 5-HT, DA and NE neurons during early developmental stage, but does not induce sprouting on these same fiber types in the adult brain.
Adaptive content delivery on mobile Internet across multiple form factors (2004)

Summary form only given. In the PC+ era, a variety of new mobile devices, such as Web-enabled watch, Smartphone, Pocket PC, Tablet PC, etc, are making a population boom. As most of current documents and multimedia contents are designed for applications on desktop PC, we are facing a big challenge - the problem of multiple form factors resulted from the diverse set of emerging new devices. For example, browsing conventional Web pages on Pocket PC is a pain. Browsing a large picture on Smartphone (e.g. picture messaging application) is no different to a thumbnail view, even though the users are now able to capture high-resolution pictures on the latest models of camera-equipped cell phones. Furthermore, enterprise software applications have been aiming to take the advantage of ubiquitous network connection by mobile Internet to increase the productivity of information workers. As the current document-related applications are highly desktop-PC focused, to unleash the power of mobile Internet in the PC+ era, we need to innovate more aggressively and develop a new generation of document and content technologies to cope with the problem of multiple form factors. In this paper, we briefly review the past research and development on adapting multimedia content for heterogeneous device capability, user context and network environment, including the MPEG-7 standard. Then, we focus on the challenge of limited display and input capability in mobile devices, we believe that in order to successfully tackle this challenge, we need to (a) develop new content representation which is scalable and adaptable, (b) develop content analysis techniques to structure content for the new representation, (c) develop corresponding rendering algorithms and innovative user interfaces to maximize the information throughput of the content on small devices, and (d) make content adaptation service part of mobile Internet architecture. We also introduce some specific research works that we have been conducting towards this direction in Microsoft Research Asia. These works include Scalable Web Document which is a new Web content representation for various display sizes, Smart Picture which uses image attention model to facilitate the browsing of large pictures on mobile devices, and Media Companion which uses proxy augmentation on the edge of mobile Internet to construct a content service overlay for dynamic adaptation and value-added services.
Human liver glycogen metabolism assessed with a 13C‐enriched diet and a 13CO2 breath test (1998)

It is possible to label liver glycogen with a diet of naturally 13C-enriched carbohydrate. The oxidation of the labelled liver glycogen can be monitored by measuring 13C-enrichment in breath CO2.