gebhart/patrepeval huggingface dataset:
Oncogenic transformation of cells by a conditionally active form of the protein kinase Akt/PKB. (2000)

The Akt/PKB protein kinase is implicated in the control of cell cycle progression and the suppression of apoptosis in cancer cells. Here we describe the use of a conditionally active form of Akt/PKB (M+ Akt:ER*) to study the ability of this protein to influence biological processes that are central to the process of oncogenic transformation of mammalian cells. Activation of M+ Akt:ER* in Rat1 cells elicited alterations in cell morphology and promoted anchorage-independent growth in agarose with high efficiency. Consistent with these observations, activation of M+ Akt:ER* suppressed the apoptosis of Rat1 cells that occurs after the detachment of these cells from extracellular matrix. Furthermore, activation of M+ Akt:ER* was sufficient to promote the progression of quiescent Rat1 cells into the S and G2-M phases of the cell cycle. In accord with this is the observation that activation of M+ Akt:ER* led to decreased expression of the cyclin-dependent kinase inhibitor p27Kip1 with a concomitant increase in cyclin-dependent kinase-2 activity. Perhaps surprisingly, activation of M+ Akt:ER* or expression of a constitutively active form of Akt led to rapid activation of MAP/ERK Kinase (MEK) and the extracellular signal-regulated kinase (ERK)/mitogen-activated protein (MAP) kinases in Rat1 cells. However, pharmacological inhibition of MEK by PD098059 did not inhibit the morphological alterations of Rat1 cells that occur after M+ Akt:ER* activation. These data suggest that M+ Akt:ER* can activate a number of pathways in Rat1 cells, leading to significant alterations in a number of biological processes. The conditional transformation system described here will allow further elucidation of the ability of Akt to contribute to both the normal response of cells to mitogenic stimulation and the aberrant proliferation observed in cancer cells.
ChemInform Abstract: A CONVENIENT SYNTHESIS OF THE AMINO ACID, 1‐AMINOCYCLOPROPANE‐1‐CARBOXYLIC ACID (1978)

Abstract N‐tert.‐Butoxycarbony1‐methionin‐methylester (I) wird mit Fluorsulfonsäuremethylester (II) methyliert und durch Behandeln mit Silberoxid und Methyliodid in den 1‐Methylaminocyclopropancarbonsäureester (III) übergeführt.
W-CDMA High Power Amplifier using Anti-Phase Intermodulation Distortion Linearization Technology (2007)

This paper shows how the ACLR of power amplifier can be reduced by using Anti-phase IMD linearization technique which generate Anti-phase IMD in the driver stage compare to output stage's IMD. Brief theory and design process are proposed. From the experimental result of 4-carriers W-CDMA input signal, this amplifier has ACLR -55 dBc@5 MHz offset at 44.7 dBm(30 watts) average output power. Compare to optimum matching technique to get maximum power gain, this technique has been improved ACLR by 12 dBc.
Isolation and serologic characterization of AIDA-I, the adhesin mediating the diffuse adherence phenotype of the diarrhea-associated Escherichia coli strain 2787 (O126:H27) (1992)

The adherence of diarrhea-associated Escherichia coli to the small-bowel mucosa is an important step in the pathogenesis of diarrheal diseases. In tissue culture systems, diarrhea-associated strains show three distinct patterns of adherence: localized adherence, diffuse adherence (DA), and the recently described aggregative adherence. To study the molecular basis of the DA phenotype, we investigated the diarrhea-associated DA strain 2787 (O126:H27), isolated from a case of infantile diarrhea. The DA phenotype is mediated by a 6.0-kb DNA fragment derived from a 100-kb plasmid harbored by the wild-type strain. This fragment codes for a 100-kDa protein which can be released from the bacterial cell into the supernatant by mild heat treatment. Recombinant DA+ strains as well as the isolated 100-kDa protein were used to engender specific antisera in rabbits. As demonstrated by Western blotting (immunoblotting), the antibodies engendered by the recombinant DA+ strain recognized a 100-kDa protein in the wild-type strain 2787 and in all recombinant strains showing DA. Immunogold electron microscopy localized the 100-kDa protein to the bacterial cell surface. Serologically related proteins of similar size were detected by Western blotting in other DA+ diarrhea-associated strains belonging to enteropathogenic E. coli serotypes. The 100-kDa protein denoted AIDA-I (adhesin involved in diffuse adherence) binds in a saturable fashion to HeLa cells. AIDA-I-specific immunoglobulin G antibodies--and, to an even greater extent, Fab fragments derived thereof--inhibited bacterial attachment to HeLa cells. This is direct evidence that the 100-kDa protein is the adhesin mediating the DA phenotype of these diarrhea-associated strains and is representative of a group of serologically related proteins in other DA+ strains.
Inhibiting Bacterial Fatty Acid Synthesis (2006)

The type II fatty acid synthase consists of a series of individual enzymes, each encoded by a separate gene, that catalyze discrete steps in chain elongation. The formation of fatty acids is vital to bacteria, and each of the essential enzymes and their acyl group carriers represent a potential target for the development of novel antibacterial therapeutics. High resolution x-ray and/or NMR structures of representative members of every enzyme in the type II pathway are now available, and these structures are a valuable resource to guide antibacterial drug discovery. The role of each enzyme in regulating pathway activity and the diversity in the components of the pathway in the major human pathogens are important considerations in deciding the most suitable targets for future drug development.
Design of super high power SAW filter (2002)

It is well known that the surface acoustic wave (SAW) filter is not well adapted to high power use. To improve its power characteristic, the use of a number of identical SAW filters to compose a parallel connection SAW filter system which has the same transmission characteristics as a single one but is durable enough for high power use is proposed. To realize this end, a matching method using the conjugate image impedances is needed to design the matching circuits of the system. In experimenting, the authors used the 800-MHz-band SAW filter to compose a two-piece, a three-piece, and a four-piece SAW filter system which can endure, respectively, 4.0 W, 6.0 W, and 8.0 W input power for more than 30 min without damage. This result also gives the possibility of designing super-high-power SAW filters using the technique described above.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Changes in the expression and distribution of the inducible and endothelial nitric oxide synthase in mucosal biopsy specimens of inflammatory bowel disease (2005)

Differences observed in the expression and distribution of NOS isoforms in immune and endothelial cells may contribute to better understanding of the structural and physiological changes in UC and CD.
A self-monitoring water bottle for tracking liquid intake (2014)

This paper presents the key concepts, system architecture, implementation details, and performance of an accelerometer-equipped bottle for monitoring and tracking liquid intake. The key system component is an elastic band, equipped with sensor and other electronics, which can be attached to a regular water bottle in order to track the bottle's usage movements. The software running on the band captures and detects acceleration signatures that the bottle experiences specifically during drinking events. Detecting such drinking events can lead to higher level monitoring such as tracking the consumed liquid volume. A Bluetooth based wireless link out of the electronic band is used for sending the detected drinking events to a smartphone or to a notebook computer for higher level tracking and data management. Different machine learning methods were adopted and experimented with for both drinking event detection and intake volume estimation. Through experiments on nine healthy subjects, the system is shown to be able to achieve up to 99% accuracy in drinking event detection, and up to 75% accuracy for intake volume estimation.
Presence of human papillomavirus in tonsillar cancer is a favourable prognostic factor for clinical outcome. (2004)

The purpose of this article is to review the current knowledge on the status and significance of human papillomavirus (HPV) in tonsillar cancer. Current data in scientific reports and data from the Karolinska Hospital and Karolinska Institute, Sweden, demonstrate that approximately half of all tonsillar cancer is HPV-positive. Moreover, patients with HPV-positive cancer have a lower risk of relapse and longer survival compared to patients with HPV-negative tonsillar cancer. The favourable outcome for patients harbouring HPV-positive tonsillar cancer cannot be attributed to increased radiosensitivity, since there is no significant difference in sensitivity to radiotherapy between HPV-positive and -negative tonsillar cancer. However, HPV-positive cancer exhibits less genetic instability i.e. shows a lower degree of aneuploidy and a tendency to have fewer chromosomal aberrations, when compared to HPV-negative tonsillar cancer.
Novel protecting groups in carbohydrate chemistry (2010)

Protecting groups play a pivotal role in carbohydrate chemistry. This review describes a selection of protecting groups and protecting group strategies that have been introduced since the beginning of this century.
Formal verification of replication on a distributed data space architecture (2002)

We investigate the formal verification of safety-critical systems on top of the distributed data space architecture Splice. In Splice each component has its own local data space which can be kept small using keys, time stamps and selective over-writing. We use two complementary formal tools: first the µCRL tool set for a rapid investigation of alternatives by a limited verification with state space exploration techniques; next the most promising solutions are verified in general by means of the interactive theorem prover of PVS. These formal techniques are used to investigate transparent replication of certain components on top of Splice. We prove that a convenient solution can be obtained by means of a slight extension of the write primitive of Splice.
Assessment of embryo viability in assisted reproductive technology: shortcomings of current approaches and the emerging role of metabolomics (2008)

As the number of assisted reproductive technology cycles increases worldwide, improvements in the ability to quickly and noninvasively determine the best embryos for transfer remain a critical goal for reproductive medicine. Recent studies suggest that metabolomic profiling of embryo culture media may provide a useful adjunct to the current embryo assessment strategies based on morphology and cleavage rate.
Handling outliers in non-blind image deconvolution (2011)

Non-blind deconvolution is a key component in image deblurring systems. Previous deconvolution methods assume a linear blur model where the blurred image is generated by a linear convolution of the latent image and the blur kernel. This assumption often does not hold in practice due to various types of outliers in the imaging process. Without proper outlier handling, previous methods may generate results with severe ringing artifacts even when the kernel is estimated accurately. In this paper we analyze a few common types of outliers that cause previous methods to fail, such as pixel saturation and non-Gaussian noise. We propose a novel blur model that explicitly takes these outliers into account, and build a robust non-blind deconvolution method upon it, which can effectively reduce the visual artifacts caused by outliers. The effectiveness of our method is demonstrated by experimental results on both synthetic and real-world examples.
Thermal Denaturation Studies of Acetylated Nucleosomes and Oligonucleosmes (1982)

The thermal melting behaviors of control and acetylated mononucleosomes, dinucleosomes and trinucleosomes have been studied. Along each series of oligonucleosomes, the melting profiles change in a manner consistent with the increasing number of nucleosomes. For the control mononucleosome, the melting profile exhibits a premelting region at about 61-64 degrees C and a major cooperative transition at 75-77 degrees C. The melting profiles of the control dinucleosomes and trinucleosomes show a premelt at 61-62 degrees C (similar to that of the nucleosome core); an intermediate transition at 73-74 degrees C for the dinucleosome and at 76-77 degrees C for the trinucleosome and a major cooperative transition at 79-80 degrees C for the dinucleosome and at 81-82 degrees C for the trinucleosome. The major cooperative transition at the highest melting temperatures in the melting profiles of the mononucleosome, dinucleosome and trinucleosome comes from the melting of the central region of DNA in the nucleosome which complexed with the core histones; the premelt region is attributed to two DNA segments per nucleosome which flank this central DNA region and are free or weakly complexed with histones. The origin of the intermediate transition found for the dinucleosomes and trinucleosomes is not fully understood but probably results from the melting of DNA at the entry to and exit from the nucleosome and the linker DNA which are complexed with histones. A very similar pattern of behavior is observed for the acetylated oligonucleosomes. Direct comparison of the melting profiles of acetylated and control mononucleosomes, dinucleosomes and trinucleosomes show that the premelt region is unaffected by histone acetylation whereas the intermediate and major cooperative transitions for the acetylated oligonucleosomes are broader and occur consistently at lower temperatures than for the controls. These differences support proposals that the N-terminal regions of core histones interact within the nucleosome and on linker DNA.
Group II muscle afferents probably contribute to the medium latency soleus stretch reflex during walking in humans (2001)

1. The objective of this study was to determine which afferents contribute to the medium latency response of the soleus stretch reflex resulting from an unexpected perturbation during human walking. 2. Fourteen healthy subjects walked on a treadmill at approximately 3.5 km h(-1) with the left ankle attached to a portable stretching device. The soleus stretch reflex was elicited by applying small amplitude (approximately 8 deg) dorsiflexion perturbations 200 ms after heel contact. 3. Short and medium latency responses were observed with latencies of 55 +/- 5 and 78 +/- 6 ms, respectively. The short latency response was velocity sensitive (P < 0.001), while the medium latency response was not (P = 0.725). 4. Nerve cooling increased the delay of the medium latency component to a greater extent than that of the short latency component (P < 0.005). 5. Ischaemia strongly decreased the short latency component (P = 0.004), whereas the medium latency component was unchanged (P = 0.437). 6. Two hours after the ingestion of tizanidine, an alpha(2)-adrenergic receptor agonist known to selectively depress the transmission in the group II afferent pathway, the medium latency reflex was strongly depressed (P = 0.007), whereas the short latency component was unchanged (P = 0.653). 7. An ankle block with lidocaine hydrochloride was performed to suppress the cutaneous afferents of the foot and ankle. Neither the short (P = 0.453) nor medium (P = 0.310) latency reflexes were changed. 8. Our results support the hypothesis that, during walking the medium latency component of the stretch reflex resulting from an unexpected perturbation is contributed to by group II muscle afferents.
Analysis of Amyloid Precursor Protein Processing Protease β‐Secretase: Tools for Memapsin 2 (β‐Secretase) Inhibition Studies (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Protease Inhibitors as Antiviral Agents (1998)

Currently, there are a number of approved antiviral agents for use in the treatment of viral infections. However, many instances exist in which the use of a second antiviral agent would be beneficial because it would allow the option of either an alternative or a combination therapeutic approach. Accordingly, virus-encoded proteases have emerged as new targets for antiviral intervention. Molecular studies have indicated that viral proteases play a critical role in the life cycle of many viruses by effecting the cleavage of high-molecular-weight viral polyprotein precursors to yield functional products or by catalyzing the processing of the structural proteins necessary for assembly and morphogenesis of virus particles. This review summarizes some of the important general features of virus-encoded proteases and highlights new advances and/or specific challenges that are associated with the research and development of viral protease inhibitors. Specifically, the viral proteases encoded by the herpesvirus, retrovirus, hepatitis C virus, and human rhinovirus families are discussed.
理想很丰满,现实很骨感 谢天谢地,苹果电视已死 (2015)

近日《华尔街日报》报道了苹果终止电视计划的消息,对此,著名IT专栏作家道森(Jan Dawson)撰文表示,苹果的电视计划就其本身而言,无甚意义,所以也算死得其所。看上去,似乎是苹果方面的一些消息源对《华尔街日报》吹了些风,后者才会写出那篇苹果已经不再汲汲于电视产品的报道的。至少
Estimating and interpreting the instantaneous frequency of a signal. I. Fundamentals (1992)

The concept of instantaneous frequency (IF), its definitions, and the correspondence between the various mathematical models formulated for representation of IF are discussed. The extent to which the IF corresponds to the intuitive expectation of reality is also considered. A historical review of the successive attempts to define the IF is presented. The relationships between the IF and the group-delay, analytic signal, and bandwidth-time (BT) product are explored, as well as the relationship with time-frequency distributions. The notions of monocomponent and multicomponent signals and instantaneous bandwidth are discussed. It is shown that these notions are well described in the context of the theory presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Autostereoscopic 3D displays (2005)

Most of the perceptual cues that humans use to visualize the world's 3D structure are available in 2D projections. This is why we can make sense of photographs and images on a television screen, at the cinema, or on a computer monitor. Such cues include occlusion, perspective, familiar size, and atmospheric haze. Four cues are missing from 2D media: stereo parallax - seeing a different image with each eye, movement parallax - seeing different images when we move our heads, accommodation - the eyes' lenses focus on the object of interest, and convergence - both eyes converge on the object of interest. All 3D display technologies (stereoscopic displays) provide at least stereo parallax. Autostereoscopic displays provide the 3D image without the viewer needing to wear any special viewing gear.
Prostate-Specific Membrane Antigen: Much More Than a Prostate Cancer Marker. (1999)

Prostate cancer continues to be the most common cancer and second leading cause of cancer-related death among men. The use of markers, particularly serum-based prostate specific antigen (PSA), has contributed to the rapid rise in diagnosed cases in the late 1980s and early 1990s, but new diagnostic and possible therapeutic markers are needed and are currently being evaluated. One of these, prostate-specific membrane antigen (PSMA), is an approximately 100-kDa type II transmembrane protein originally thought to be highly selectively expressed in all types of prostatic tissue, with expression being upregulated in androgen-depleted or androgen-independent states. The radioimmunoconjugate form of the anti-PSMA monoclonal antibody (mAb) 7E11 is currently being used to diagnose prostate cancer metastasis and recurrence. In addition, Phase I and II trials have started utilizing PSMA in different therapeutic ways, with promising results. Recent exciting work has demonstrated PSMA expression in endothelial cells of vessels restricted to the tumor-associated neovasculature. This finding expands the possible beneficial uses of PSMA, as new anti-PSMA mAbs continue to be developed.
Inhibition of Rous sarcoma viral RNA translation by a specific oligodeoxyribonucleotide. (1978)

A tridecamer oligodeoxynucleotide, d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to reiterated 3'- and 5'-terminal nucleotides of Rous sarcoma virus 35S RNA, is an efficient inhibitor of the translation of proteins specified by the viral RNA in the wheat embryo cell-free system. The inhibition specificity for oncornavirus RNA is greater than for rabbit reticulocyte mRNA or brome mosaic virus RNA. Other oligodeoxynucleotides of similar size have little or no specific effect on the RNA-directed translation. The tridecamer acts as a primer for the avian myeloblastosis virus DNA polymerase when Rous sarcoma virus heated 70S RNA is used as a template, offering evidence that it can hybridize to the RNA. The possible use of such an oligodeoxynucleotide hybridization competitor to inhibit Rous sarcoma virus replication is described in the preceding paper [Zamecnik, P. C. & Stephenson, M. L. (1978) Proc. Natl. Acad. Sci. USA. 75, 280--284].
Quantitative microwave imaging with a 2.45-GHz planar microwave camera (1998)

This paper presents microwave tomographic reconstructions of the complex permittivity of lossy dielectric objects immersed in water from experimental multiview near-field data obtained with a 2.45-GHz planar active microwave camera. An iterative reconstruction algorithm based on the Levenberg-Marquardt method was used to solve the nonlinear matrix equation which results when applying a moment method to the electric field integral representation. The effects of uncertainties in experimental parameters such as the exterior medium complex permittivity, the imaging system geometry and the incident field at the object location are illustrated by means of reconstructions from synthetic data. It appears that the uncertainties in the incident field have the strongest impact on the reconstructions. A receiver calibration procedure has been implemented and some ways to access to the incident field at the object location have been assessed.
Person-of-interest detection system using cloud-supported computerized-eyewear (2013)

Detecting Person-Of-Interest (POI), e.g., fugitives, criminals and terrorists in public spaces is a critical requirement of many law enforcers and police officers. In realty, most law enforcement personnel cannot effectively differentiate POIs from millions of faces and thus demand a portable assistant to recognize faces, in order to take the golden opportunity taking the POIs into immediate custody. Unfortunately, current face recognition systems are stationary and limited to a small scale of POI datasets. In this paper, we investigate a wearable computerized-eyewear based face recognition system. This system is a portable device which can accompany a police officer during patrolling or other tasks. The eyewear is connected to a cloud based face recognition system via wireless networks. Facial images captured by the mounted camera are sent to the cloud for identity retrieval. When the system finds a POI, it would alert officers via overlaying a virtual identity tag on the real POI's face on the transparent screen of the eyewear. We provide approaches to greatly minimize recognition time, including leveraging the large storage and high computational capacities provided by the cloud. The cloud enables nationwide POI database and supports parallel computing for face recognition.