gebhart/patrepeval huggingface dataset:
“Clinging‐Microdroplet” Patterning Upon High‐Adhesion, Pillar‐Structured Silicon Substrates (2011)

Abstract The rapidly increasing research interest in nanodevices, including nanoelectronics, nano‐optoelectronics, and sensing, requires the development of surface‐patterning techniques to obtain large‐scale arrays of nanounits (mostly nanocrystals and/or nanoparticles) on a silicon substrate. Herein, we demonstrate a “clinging‐microdroplet” method to fabricate patterning crystal arrays based on the employment of high‐adhesion, superhydrophobic, pillar‐structured silicon substrates. Different from the previous hydrophilic/hydrophobic patterned self‐assembly monolayer technique, this method provides a novel strategy to fabricate patterning crystal arrays upon pillar‐structured silicon substrates of homogenous superhydrophobicity and high adhesion, which greatly simplifies the modification process of the supporting substrates. Ordered crystal arrays with a tunable size and distribution density were successfully generated, and individual crystals grew on the top of each micropillar. Besides soluble inorganic materials, protein microspheres and suspending Ag‐nanoparticle or polystyrene‐microsphere aggregations could also be patterned in regular arrays, showing the wide adaptation of such an adhesive patterning technique. This novel and low‐cost technique for patterning crystal arrays upon silicon substrates could yield breakthroughs in areas ranging from nanodevices to nanoelectronics.
A Comparison of Whole-Genome Shotgun-Derived Mouse Chromosome 16 and the Human Genome (2002)

The high degree of similarity between the mouse and human genomes is demonstrated through analysis of the sequence of mouse chromosome 16 (Mmu 16), which was obtained as part of a whole-genome shotgun assembly of the mouse genome. The mouse genome is about 10% smaller than the human genome, owing to a lower repetitive DNA content. Comparison of the structure and protein-coding potential of Mmu 16 with that of the homologous segments of the human genome identifies regions of conserved synteny with human chromosomes (Hsa) 3, 8, 12, 16, 21, and 22. Gene content and order are highly conserved between Mmu 16 and the syntenic blocks of the human genome. Of the 731 predicted genes on Mmu 16, 509 align with orthologs on the corresponding portions of the human genome, 44 are likely paralogous to these genes, and 164 genes have homologs elsewhere in the human genome; there are 14 genes for which we could find no human counterpart.
The complete genome sequence of a dog: a perspective (2006)

A complete, high-quality reference sequence of a dog genome was recently produced by a team of researchers led by the Broad Institute, achieving another major milestone in deciphering the genomic landscape of mammalian organisms. The genome sequence provides an indispensable resource for comparative analysis and novel insights into dog and human evolution and history. Together with the survey sequence of a poodle previously published in 2003, the two dog genome sequences allowed identification of more than 2.5 million single nucleotide polymorphisms within and between dog breeds, which can be used in evolutionary analysis, behavioral studies and disease gene mapping.(1)
Inhibition of serum phospholipase-A2in acute pancreatitis by pharmacological agentsin vitro (1997)

Phospholipase-A2 has been suggested as having a role in the pathophysiology of acute pancreatitis. The inhibition of phospholipase-A2 was studied in vitro using 17 pharmacological agents in the search for a specific therapy for acute pancreatitis. The inhibitory effect was tested using an isotopic assay system with 2-palmitoyl-(1-14C)-labelled dipalmitoyl phosphatidylcholine as a substrate and 10 microliters of serum from patients with acute necrotizing pancreatitis as an enzyme source. Among all agents tested, anti-inflammatory drugs inhibited enzyme activity most significantly: indomethacin (9.0 x 10(-3) mol l-1) decreased the phospholipase-A2 activity to one- tenth. The weak inhibitory effect could also be demonstrated using a lower concentration of 2 x 10(-5) mol l-1, which can be achieved after intravenous administration of 50 mg of this drug. The other drugs inhibited the enzyme activity at concentrations higher than those achieved after intravenous injections in clinical use. Diclofenac (3.1 x 10(-2) mol l-1) reduced the phospholipase-A2 activity by 93%, ketoprofen (2.0 x 10(-2) mol l-1) or chlorpromazine (1.4 x 10(-2) mol l-1) by 90%, tobramycin (1.7 x 10(-2) mol l-1) by 84%, doxycycline (9.0 x 10(-3) mol l-1) by 61%, dexamethasone (1.7 x 10(-3) mol l-1) by 62%, methylprednisolone (3.8 x 10(-2) mol l-1) by 50%, and pindolol (1.0 x 10(-4) mol l-1) by 59%. A weak inhibition of phospholipase-A2 activity was demonstrated by betamethasone, bupivacaine, digoxin, hydrocortisone, lidocaine, metoprolol, propranolol, and vancomycin. Indomethacin proved the most potent of the tested agents in inhibiting phospholipase-A2 activity in serum from patients with acute pancreatitis and should be further studied in vivo.
Fibroblasts as Efficient Antigen-Presenting Cells in Lymphoid Organs (1995)

Only so-called "professional" antigen-presenting cells (APCs) of hematopoietic origin are believed capable of inducing T lymphocyte responses. However, fibroblasts transfected with viral proteins directly induced antiviral cytotoxic T lymphocyte responses in vivo, without involvement of host APCs. Fibroblasts induced T cells only in the milieu of lymphoid organs. Thus, antigen localization affects self-nonself discrimination and cell-based vaccine strategies.
Woodchuck Hepatitis Virus Contains a Tripartite Posttranscriptional Regulatory Element (1998)

The hepatitis B virus posttranscriptional regulatory element (HBVPRE) is a cis-acting RNA element that partially overlaps with enhancer I and is required for the cytoplasmic accumulation of HBV surface RNAs. We find that the closely related woodchuck hepatitis virus (WHV), which has been shown to lack a functional enhancer I, also contains a posttranscriptional regulatory element (WPRE). Deletion analysis suggests that the WPRE consists of three independent subelements. Comparison of the bipartite HBVPRE and tripartite WPRE activities reveals that the tripartite WPRE is two to three times more active than the bipartite HBVPRE. Mutation of a single WPRE subelement decreases WPRE activity to the level of the HBVPRE. Bipartite and tripartite chimeras of the WPRE and HBVPRE possess activities which suggest that elements containing three subelements are posttranscriptionally stronger than those containing two. These data demonstrate that the posttranscriptional regulatory element is conserved within the mammalian hepadnaviruses and that its strength is determined by the number of subelements within the RNA.
Histone Deacetylase Activity Regulates Chemical Diversity in Aspergillus (2007)

Bioactive small molecules are critical in Aspergillus species during their development and interaction with other organisms. Genes dedicated to their production are encoded in clusters that can be located throughout the genome. We show that deletion of hdaA, encoding an Aspergillus nidulans histone deacetylase (HDAC), causes transcriptional activation of two telomere-proximal gene clusters--and subsequent increased levels of the corresponding molecules (toxin and antibiotic)--but not of a telomere-distal cluster. Introduction of two additional HDAC mutant alleles in a DeltahdaA background had minimal effects on expression of the two HdaA-regulated clusters. Treatment of other fungal genera with HDAC inhibitors resulted in overproduction of several metabolites, suggesting a conserved mechanism of HDAC repression of some secondary-metabolite gene clusters. Chromatin regulation of small-molecule gene clusters may enable filamentous fungi to successfully exploit environmental resources by modifying chemical diversity.
A Reliable Method for Intratracheal Instillation of Materials to the Entire Lung in Rats (2006)

A novel method for intratracheal instillation of rats is described by which an operator can confidently administer a liquid sample into the tracheal lumen, with direct observation of the entrance to the trachea. To confirm its efficacy, indigo carmine was administered with or without air to investigate optimal conditions for exposure of the whole lung. After administration of 0.5 ml of indigo carmine and an equal volume of air simultaneously by intratracheal instillation, sections through the whole lung demonstrated positive staining with the dye. For histological examination, 0.5 ml of saline containing iron oxide followed by an equal volume of air was administered intratracheally. Iron particles were observed in the wall of bronchioles and alveolar macrophages of whole lung. The method allows reliable intratracheal administration to large numbers of rats within a short period and should, therefore, prove useful for assays of toxicity in the respiratory tract.
Power-dense multilevel inverter module using interleaved GaN-based phases for electric aircraft propulsion (2018)

Realizing the electrification of aircraft necessitates the development of inverters and rectifiers with ultra-high efficiency and specific power simultaneously. Unconventional topologies such as the flying capacitor multilevel (FCML) inverter hold great promise for achieving transformational reductions in inverter power loss and weight due to drastically smaller filters and compact hybrid energy transfer mechanism. After describing methods to overcome obstacles in ultra-efficient and lightweight converter design, this work presents an example of interleaved FCML inverter operation. The successful, balanced interleaved operation was demonstrated in the laboratory with a dual-interleaved, 9-level converter prototype at 1 kV input bus voltage and 9.7 kW output power. At 98.6% peak efficiency, this converter achieves a gravimetric power density of 17.3 kW/kg and a volumetric power density of 35.3 kW/L including the contributions of the heat sink. Interestingly, observations from loss modeling and hardware efficiency measurements revealed the large impact of second-order loss mechanisms, such as dynamic on-state resistance and junction temperature. Insights gained from this new understanding can be incorporated into future modeling for more thorough optimization of the converter design.
Dynamics of rigid bodies undergoing multiple frictional contacts (2003)

Two key problem areas in the dynamics of rigid bodies with multiple frictional contacts are solved. First, the modeling of rigid body collisions is addressed. Second, an accurate model that will predict the contact forces is sought. The emphasis is on correct phenomenological and quantitative modeling. An approach to the simulation of mechanical systems with multiple, frictional constraints is proposed which is free of inconsistencies. This method is illustrated with the help of a simple planar example.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
MMP‐2 and MMP‐9 synergize in promoting choroidal neovascularization (2003)

Matrix metalloproteinase 2 (MMP-2) and MMP-9 are increased in human choroidal neovascularization (CNV) occurring during the exudative most aggressive form of age-related macular degeneration (AMD), but their precise role and potential interactions remain unclear. To address the question of MMP-2 and MMP-9 functions, mice deficient in the expression of MMP-2 (MMP-2 KO), MMP-9 (MMP-9 KO), and both MMP-2 and MMP-9 (MMP-2,9 KO) with their corresponding wild-type mice (WT) underwent CNV induction by laser-induced rupture of the Bruch's membrane. Both the incidence and the severity of CNV were strongly attenuated in double deficient compared with single gene deficient mice or corresponding WT controls. The reduced neovascularization was accompanied by fibrinogen/fibrin accumulation. Furthermore, overexpression of the endogenous MMP inhibitors TIMP-1 or TIMP-2 (delivered by adenoviral vectors) in WT mice or daily injection of a synthetic and gelatinase selective MMP inhibitor (Ro 26-2853) significantly decreased the pathological reaction. These findings suggest that MMP-2 and MMP-9 may cooperate in the development of AMD and that their selective inhibition represents an alternative strategy for the treatment of choroidal neovascularization.
Patch clamp reveals powerful blockade of the mitochondrial permeability transition pore by the D2‐receptor agonist pramipexole (2006)

The dopamine-D2-agonist pramipexole (PPX) was tested for blocking mitochondrial permeability transition (PT) in order to give a possible explanation for its neuroprotective effect seen in PPX-treated Parkinson's disease patients. Patch-clamp techniques for studying single-channel currents in the inner mitochondrial membrane and large-amplitude swelling of energized mitochondria were used to study PPX action on the permeability transition pore (PTP), a key player in the mitochondrial route of the apoptotic cascade. Identity of the PTP was proven by measuring the concentration-response relation for cyclosporin A-blockade (IC50=26 nM). PPX inhibits the PTP reversibly with an IC50 of 500 nM, which is close to the values determined earlier as plasma concentrations after PPX medication in patients. Interaction of PPX with the PTP is further supported by demonstrating that it abolished Ca2+-triggered swelling in functionally intact mitochondria. Blockade of the PTP by PPX was attenuated by increasing concentrations of inorganic phosphate and by acidification. We suggest that PPX could exert part of its neuroprotective effect by inhibition of the PTP and thus, probably, blocking of the mitochondrial pathway of the apoptosis cascade.
A Thrombin-Sensitive Protein of Human Platelet Membranes (1971)

The action of thrombin on intact human platelets has been studied with the aid of polyacrylamide gel electrophoresis in sodium dodecylsulfate. A single major membrane protein band with a molecular weight of 190,000 disappears after thrombin treatment, while a new membrane protein with a molecular weight of 107,000 appears. This may represent hydrolysis of the thrombin-sensitive protein. When platelets are disrupted or when the thrombin-sensitive protein is solubilized from membranes prior to thrombin treatment, no hydrolysis occurs. The effect of thrombin on the platelet membrane protein is complete within 2 min which suggests that hydrolysis of this membrane protein may trigger the physiological effects of thrombin on platelets.
Quantum Dot-Based OFF/ON Probe for Detection of Glutathione (2009)

A simple strategy for detection of glutathione (GSH) in physiologically relevant concentrations is reported by manipulating the electron transfer pathways of surface-modified quantum dots. The probe consisted of fluorescent CdS:Mn/ZnS core/shell quantum dots (Qdot) properly conjugated to dopamine through CS2-assisted zero-length covalent coupling. Qdots exhibited bright yellow-orange emission at ∼ 592 nm, which could be attributed to the 4T1 → 6A1 transition of Mn2+ ions in Qdot core. The fluorescently dark Qdots (OFF state) was obtained by modifying the Qdot surface with an electron rich dopamine molecule. The fluorescence of the probe was restored (ON state) when the disulfide bond was cleaved by the reducing GSH.
Transpulmonary pyruvate kinetics (2011)

Shuttling of intermediary metabolites, such as pyruvate, contributes to the dynamic energy and biosynthetic needs of tissues. Tracer kinetic studies offer a powerful tool to measure the metabolism of substrates like pyruvate that are simultaneously taken up from and released into the circulation by organs. However, we understood that during each circulatory passage, the entire cardiac output transits the pulmonary circulation. Therefore, we examined the transpulmonary pyruvate kinetics in an anesthetized rat model during an unstimulated (Con), lactate clamp (LC), and epinephrine infusion (Epi) conditions using a primed-continuous infusion of [U-¹³C]pyruvate. Compared with Con and Epi stimulation, LC significantly increased mixed central venous ([v]) and arterial ([a]) pyruvate concentrations (P < 0.05). We hypothesized that the lungs, specifically the pulmonary capillary beds are sites of simultaneous production and removal of pyruvate and contributes significantly to whole body carbohydrate intermediary metabolism. Transpulmonary net pyruvate balances were positive during all three conditions, indicating net pyruvate uptake. Net balance was significantly greater during epinephrine stimulation compared with the unstimulated control (P < 0.05). Tracer-measured pyruvate fractional extraction averaged 42.8 ± 5.8% for all three conditions and was significantly higher during epinephrine stimulation (P < 0.05) than during either Con or LC conditions, that did not differ from each other. Pyruvate total release (tracer measured uptake - net balance) was significantly higher during epinephrine stimulation (400 ± 100 μg/min) vs. Con (30 ± 20 μg/min) (P < 0.05). These data are interpreted to mean that significant pyruvate extraction occurs during circulatory transport across lung parenchyma. The extent of pulmonary parenchymal pyruvate extraction predicts high expression of monocarboxylate (lactate/pyruvate) transporters (MCTs) in the tissue. Western blot analysis of whole lung homogenates detected three isoforms, MCT1, MCT2, and MCT4. We conclude that a major site of circulating pyruvate extraction resides with the lungs and that during times of elevated circulating lactate, pyruvate, or epinephrine stimulation, pyruvate extraction is increased.
The Sir2 Family of Protein Deacetylases (2004)

The yeast SIR protein complex has been implicated in transcription silencing and suppression of recombination. The Sir complex represses transcription at telomeres, mating-type loci, and ribosomal DNA. Unlike SIR3 and SIR4, the SIR2 gene is highly conserved in organisms ranging from archaea to humans. Interestingly, Sir2 is active as an NAD+-dependent deacetylase, which is broadly conserved from bacteria to higher eukaryotes. In this review, we discuss the role of NAD+, the unusual products of the deacetylation reaction, the Sir2 structure, and the Sir2 chemical inhibitors and activators that were recently identified. We summarize the current knowledge of the Sir2 homologs from different organisms, and finally we discuss the role of Sir2 in caloric restriction and aging.
Timing verification on a 1.2M-device full-custom CMOS design (1991)

Static timing verification on a large full-custom design is an extremely challenging problem for which no complete solution exists. In this paper, we present our experience on the timing verification of our next generation CMOS CPU design using an internally developed tool,
Can sorting using sheet-of-light range imaging and MAPP2200 (2002)

This report describes a real-time can sorting application using a sheet-of-light range imaging algorithm on the MAPP2200 smart sensor camera. The camera outputs range data with a maximum frequency of 800 KHz. The range data are automatically corrected, and uncertain data are removed. We acquire range images of fish conserves passing on a conveyor belt and finds the orientation of up to ten cans per second.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Growth factors influence the sensitivity of leukemic stem cells to cytosine arabinoside in culture (1989)

We have proposed that the blasts in acute myeloblastic leukemia (AML) are renewal populations maintained by a small subpopulation of stem cells. The balance between self-renewal and differentiation in blast stem cells may be an important attribute contributing to treatment outcome. Cytosine arabinoside (ara-C) is included in most chemotherapeutic regimens for the treatment of AML. When ara-C survival curves are constructed, the drug appears to be more toxic when an assay is used that detects principally self-renewing divisions, compared with a procedure that depends on terminal divisions. AML blasts usually respond in culture to myelopoietic growth factors; their response often includes a change in self-renewal, differentiation, or both. These features of the model for AML blasts led to the prediction that growth factors would alter ara-C survival curves in a way that depended on the effects of the culture conditions on self-renewal and differentiation. Four AML blast populations were chosen to test this prediction on the basis of our ability to manipulate them by adding or withholding one or more growth factors. Highly significant changes were seen in the ara-C survival curves, depending on the growth factors present in the cultures as was predicted by the observed effects of the factors on renewal and differentiation.
Single-walled carbon nanotubes growing radially from YC2 particles (1994)

In the primary soot produced by arc discharge using an yttrium carbide loaded anode, bundles of single-walled carbon nanotubes (SWT) are observed, protruding radially from YC2 particles coated with graphitic multilayers. The graphitic cages separating YC2 particle and SWT bundles fall into the narrow range of 10–20 layers. The morphology of the clusters suggests a two-step growth model: The radial SWT growth pattern is first initiated by catalytic action between the YC2 droplet and the carbon in the gas phase. Second, and upon cooling, the graphitic cage starts by segregating excess carbon from the YC2 bulk, arresting further growth of SWT.
Report of the Northeast Modern Language Association (1988)

The 1989 NEMLA convention will be held 31 March-2 April at the Radisson Hotel in Wilmington, Delaware, with the University of Delaware as the host institution. The local committee is chaired by Joan L. Brown and Joan Del Fattore (Univ. of Delaware). Information about the convention may be obtained from NEMLA President F. William Forbes, Dept. of Spanish and Classics, Univ. of New Hampshire, Durham 03824.