gebhart/patrepeval huggingface dataset:
Probing of nucleic acids by metal ion complexes of small molecules (1996)

Molecular modelling of transition metal adducts with nucleic acids and their constituents zinc complexes as targeting agents for nucleic acids metallocene interactions with DNA and DNA processing enzymes evidences for a catalytic activity of the DNA double helix in the reactions between DNA, platinum (II) and intercalators trans-diammineplatinum (II) - what makes it different from CIS-DDP? coordination chemistry of a neglected relative of cisplatin and its interaction with nucleic acids metal ions in multiple-stranded DNA DNA interactions with substitution-intert transition metal ion complexes effect of metal ions on the fluorescence of dyes bound to DNA photolytic covalent binding of metal complexes to DNA electrochemically activated nucleic acid oxidation electron transfer between metal complexes bound to DNA - is DNA a wire? porphyrin and metalloporphyrin interactions with DNA selective DNA cleavage by metalloporphyrin derivatives synthetic metalloporphyrins as probes of protein-DNA interactions targeting of nucleic acids by iron complexes nucleic acid chemistry of the cuprous complexes of 1,10-phenanthroline and derivatives specific DNA cleavage by manganese (III) complexes nickel complexes as probes of guanine sites in nucleic acid folding hydrolytic cleavage of RNA catalyzed by metal ion complexes RNA recognition and cleavage by iron (III)-bleomycin metallobleomycin-DNA interactions - structures and reactions related to bleomycin-induced DNA damage.
Counter-flow elutriation of clinical peripheral blood mononuclear cell concentrates for the production of dendritic and T cell therapies (2014)

Elutriation was consistent and effective for isolating monocytes and lymphocytes from PBMC concentrates for manufacturing clinical cell therapies, but further processing is often required.
The Protein Activator Specific for the Enzymic Hydrolysis of GM2 Ganglioside in Normal Human Brain and Brains of Three Types of GM2 Gangliosidosis (1983)

In order to understand the etiology of Type AB GM2 gangliosidosis, we have purified and characterized the activator protein (GM2 activator) specific for the enzymic hydrolysis of GM ganglioside from normal human brain. The purified activator from human brain moved as one major protein band in various electrophoretic systems. We have also prepared the antiserum against this activator. The levels and the nature of GM2 activator and beta-hexosaminidase A were examined in the brains of five cases of GM2 gangliosidosis-one Type B, two O, and two Type AB. We found that the levels of GM2 activator in both Type B and Type O cases were markedly elevated, and that the two Type AB cases were the results of different causes. One case had a defective beta-hexosaminidase A and an elevated level of GM2 activator. Although this defective beta-hexosaminidase A could hydrolyze synthetic substrates, it was inactive in the cleavage of natural glycosphingolipids in the presence of the GM2 activator. It could, however, hydrolyze asialo-GM2 and GbOse4Cer in the presence of sodium taurodeoxycholate. The other case had normal beta-hexosaminidase A, but had a very low level of GM2 activator when analyzed by in vitro assay, suggesting the deficiency of this activator. By immunoelectrophoresis, this case was found to be completely devoid of the protein that cross-reacts with the antiserum against the GM2 activator.
Comparison of the effects of alcohol and sleepiness on simple reaction time performance: Enhanced habituation as a common process (1990)

This paper examines the extent to which the performance-impairing effects of alcohol and sleepiness have been evaluated as a function of time-on-task to determine the relevance of accelerated habituation in the bahavioral deficits observed. It is concluded that despite large research literatures on the effects of alcohol and sleepiness on performance, the relevance of habituation to driving, and the need to know whether alcohol and sleepiness potentiate the effects of each other, very little work has evaluated performance as a function of time-on-task. What has been done using sustained simple reaction time (SRT) suggests that at high doses both alcohol and sleep loss accelerate whether the two processes interact. We conclude by suggesting that consideration of the role of habituation in the behavioral effects of alcohol and sleepiness requires formulations based on the interaction between the impaired driver and the environment.
Acylated 1,3-Aminopropanols as Repellents against Bloodsucking Arthropods (1996)

Starting from a set of known repellent compounds, a general structural framework with high probability for repellent activity was developed by molecular modelling techniques. Synthesis, structure–biological activity relationships of acylated 1,3-aminopropanols on the yellow fever mosquito Aedes aegypti and the properties and activity of the new development candidate compound KBR 3023 on Ae. aegypti, the malaria mosquito Anopheles stephensi, the common house mosquito Culex quinquefasciatus and the stable fly Stomoxys calcitrans are described. The sensory effect of KBR 3023 and deet has been studied in behavioural and neurophysiological investigations on Ae. aegypti and the American cockroach Periplaneta americana. The compounds clearly reduce or even eliminate the approach behaviour towards attractants like host odours or sexual pheromones. Electrophysiological studies on the insects' olfactory receptor organs reveal that certain cell types, which are not involved in perception of the attractive odorants, respond to deet and/or KBR 3023. As soon as one of the compounds is presented together with an attractant, a new input is active in the brain, which adds to the input from other receptors activated by the attractant. This new overall pattern clearly differs from that elicited by the attractant, so that the insect is no longer able to detect the latter. The specificity and mode of action of KBR 3023 was investigated by experiments exploring second-messenger responses elicited in antennal preparations of male P. americana. KBR 3023 induced a rapid increase in the concentration of inositol triphosphate in a dose-dependent and tissue-specific manner; other second-messenger systems were not affected. These observations suggest that KBR 3023 may act via subsets of G-protein-coupled receptors in sensory neurones.
Lipids in gap junction assembly and function (1990)

Gap junctions (GJ) are important regulators of cellular function. They provide channels for the direct movement of small molecules between cells and thus control cell-to-cell transfer of metabolites and the transmission of various stimuli. Gap junctions have been shown to be involved in a multitude of cellular processes ranging from cell synchronization and neuronal function to cell differentiation and carcinogenesis. Much knowledge has been gained in recent years concerning the structure and molecular organization of GJ proteins; yet, the mechanisms that control and modulate gap junction assembly and function are still not well understood. Although it is quite apparent that the GJ proteins assemble in the lipid milieu of the plasma membrane, and that the cluster of proteins assembled in the junction do function in a lipid environment, there is a general paucity of information on the role of lipids in the gap junction assembly process and in the function of gap junctions. The present review is a comprehensive account of current knowledge on gap junction lipids. We also discuss what is known to date on the involvement of lipids in gap junction formation. Special emphasis is being placed on the potential role of membrane cholesterol in gap junction assembly and function.
First Clinical Evidence That Imaging with Somatostatin Receptor Antagonists Is Feasible (2011)

Imaging of neuroendocrine tumors with sst antagonists is clinically feasible. The favorable human biodistribution data suggest that sst antagonists could significantly affect peptide receptor-mediated imaging and therapy.
Clinical Significance of IL-23 Regulating IL-17A and/or IL-17F Positive Th17 Cells in Chronic Periodontitis (2014)

This study indicates that both the IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 cells may be involved in pathogenesis of periodontitis. The role of these Th17 cells in the disease pathogenesis needs to be further investigated.
Synthesis and biological activity of 3-(N-substituted pyridinium-4-thiomethyl)-7.ALPHA.-formamido cephalosporins. (1993)

The synthesis and antibacterial activity of a series of 3-(1-substituted pyridinium-4-thiomethyl)-7 alpha-formamido cephalosporins is described. All the derivatives showed good potency and stability to bacterial beta-lactamases. The antibacterial efficacy seen with the N-alkyl pyridinium substituents was enhanced by the introduction of a catecholic side chain at C-7 and by preparation of N-(substituted amino)pyridinium derivatives.
Design of Analog CMOS Integrated Circuits (1999)

The CMOS technology area has quickly grown, calling for a new text--and here it is, covering the analysis and design of CMOS integrated circuits that practicing engineers need to master to succeed. Filled with many examples and chapter-ending problems, the book not only describes the thought process behind each circuit topology, but also considers the rationale behind each modification. The analysis and design techniques focus on CMOS circuits but also apply to other IC technologies.
Table of contents

1 Introduction to Analog Design
2 Basic MOS Device Physics
3 Single-Stage Amplifiers
4 Differential Amplifiers
5 Passive and Active Current Mirrors
6 Frequency Response of Amplifiers
7 Noise
8 Feedback
9 Operational Amplifiers
10 Stability and Frequency Compensation
11 Bandgap References
12 Introduction to Switched-Capacitor Circuits
13 Nonlinearity and Mismatch
14 Oscillators
15 Phase-Locked Loops
16 Short-Channel Effects and Device Models
17 CMOS Processing Technology
18 Layout and Packaging
Fuzzy adaptive connection admission control for real-time applications in ATM-based heterogeneous networks (1999)

In this paper, we study connection-oriented communication service in heterogeneous network for real-time applications. Many existing distributed mission-critical systems are deployed over heterogeneous networks. Hence, it is necessary to extend the real-time communication technology to encompass heterogeneous networks. A connection can be considered as a contract between an application and the network: the application specifies the characteristics of the traffic which it may generate and the network agrees to provide the requested quality of service (QoS) to the application. For real-time applications, the most crucial QoS is to meet deadline requirement. We propose a fuzzy intelligent system for connection admission control (CAC). Upon a request of connection establishment, the CAC determines if the worst case delays of the requesting and existing connections can be satisfied given the available network resources. If so, the CAC allocates appropriate network resources to the requesting connection. Our system uses fuzzy logic to capture the knowledge for adapting its strategy to dynamic system status. Our performance study demonstrates that system performance can be improved significantly and consistently with our fuzzy adaptive connection admission control. Our approach is compatible with current network standards and hence can be readily used in practical systems.
A Clonogenic Bone Marrow Progenitor Specific for Macrophages and Dendritic Cells (2005)

Macrophages and dendritic cells (DCs) are crucial for immune and inflammatory responses and belong to a network of cells that has been termed the mononuclear phagocyte system (MPS). However, the origin and lineage of these cells remain poorly understood. Here, we describe the isolation and clonal analysis of a mouse bone marrow progenitor that is specific for monocytes, several macrophage subsets, and resident spleen DCs in vivo. It was also possible to recapitulate this differentiation in vitro by using treatment with the cytokines macrophage colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. Thus, macrophages and DCs appear to renew from a common progenitor, providing a cellular and molecular basis for the concept of the MPS.
Dihydroorotate dehydrogenase inhibitor A771726 (leflunomide) induces apoptosis and diminishes proliferation of multiple myeloma cells (2009)

Multiple myeloma is still an incurable disease; therefore, new therapeutics are urgently needed. A771726 is the active metabolite of the immunosuppressive drug leflunomide, which is currently applied in the treatment of rheumatoid arthritis, BK virus nephropathy, and cytomegaly viremia. Here, we show that dihydroorotate dehydrogenase (DHODH) is commonly expressed in multiple myeloma cell lines and primary multiple myeloma cells. The DHODH inhibitor A771726 inhibits cell growth in common myeloma cell lines at clinically achievable concentrations in a time- and dose-dependent manner. Annexin V-FITC/propidium iodide staining revealed induction of apoptosis of multiple myeloma cell lines and primary multiple myeloma cells. The 5-bromo-2'-deoxyuridine cell proliferation assay showed that inhibition of cell growth was partly due to inhibition of multiple myeloma cell proliferation. A771726 induced G(1) cell cycle arrest via modulation of cyclin D2 and pRb expression. A771726 decreased phosphorylation of protein kinase B (Akt), p70S6K, and eukaryotic translation initiation factor 4E-binding protein-1 as shown by Western blotting experiments. Furthermore, we show that the stimulatory effect of conditioned medium of HS-5 bone marrow stromal cells on multiple myeloma cell growth is completely abrogated by A771726. In addition, synergism studies revealed synergistic and additive activity of A771726 together with the genotoxic agents melphalan, treosulfan, and doxorubicin as well as with dexamethasone and bortezomib. Taken together, we show that inhibition of DHODH by A771726/leflunomide is effective in multiple myeloma. Considering the favorable toxicity profile and the great clinical experience with leflunomide in rheumatoid arthritis, this drug represents a potential new candidate for targeted therapy in multiple myeloma.
Inhibition of membrane‐bound lytic transglycosylase B by NAG‐thiazoline (2004)

The lytic transglycosylases cleave the bacterial cell wall heteropolymer peptidoglycan with the same specificity as the muramidases (lysozymes), between the N-acetylmuramic acid and N-acetylglucosamine residues, with the concomitant formation of a 1,6-anhydromuramoyl residue. The putative catalytic residue in the family 3 lytic transglycosylase from Pseudomonas aeruginosa, Glu162 as identified by sequence alignment to the homologous enzyme from Escherichia coli, was replaced with both Ala and Asp by site-directed mutagenesis. Neither mutant enzyme differed structurally from the wild-type enzyme, as judged by CD spectroscopy, but both were enzymatically inactive confirming the essential role of Glu162 in the mechanism of action of this lytic transglycosylase. The beta-hexosaminidase inhibitor NAG-thiazoline was shown to inhibit the activity of lytic transglycosylase activity, thus providing the first direct evidence that the formation of the 1,6-anhydromuramoyl residue may proceed through an oxazolinium ion intermediate involving anchimeric assistance. Using surface plasmon resonance and difference absorbance spectroscopy, Kd values of 1.8 and 1.4 mM, respectively, were determined for NAG thiazoline, while its parent compound N-acetylglucosamine neither inhibited nor appeared to bind the lytic transglycosylase with any significant affinity.
Future of Theranostics: An Outlook on Precision Oncology in Nuclear Medicine (2019)

Molecular alterations in malignant disease result in the expression or upregulations of various targets that can be used for imaging and treatment with radiopharmaceuticals. This theranostic principle has acquired greater importance in personalized medicine in recent years, particularly in oncology, where advanced tumors can be treated effectively with low side effects. Since the pioneering use of <sup>131</sup>I in differentiated thyroid cancer in the 1940s, remarkable achievements in nuclear medicine endoradiotherapy have been demonstrated, mainly in the treatment of neuroendocrine neoplasms by using <sup>177</sup>Lu-labeled somatostatin analogs or in the treatment of advanced prostate cancer using prostate-specific membrane antigen-directed radionuclide therapy. Besides that, this review focuses on promising novel radiopharmaceuticals and describes their preclinical and clinical status. Radiolabeled antibodies, such as <sup>131</sup>I-omburtamab directed against the B7-H3 protein on the surface of neuroblastoma cells; HuMab-5B1, a <sup>89</sup>Zr/<sup>177</sup>Lu-labeled antibody for the treatment of CA19-9-expressing malignancies; and <sup>177</sup>Lu-lilotomab, a CD37 antibody for the treatment of B-cell lymphomas, are being highlighted. The neurotensin receptor ligand <sup>111</sup>In/<sup>177</sup>Lu-3B-227 has demonstrated high potential in imaging and therapy for several malignancies (e.g., pancreatic adenocarcinomas). Targeting of the fibroblast activation protein is currently being explored for different tumor entities using PET imaging with the fibroblast activation protein inhibitor (FAPI) <sup>68</sup>Ga-FAPI-04, and the first therapeutic applications of <sup>90</sup>Y-FAPI-04 have been applied. After 2 decades of rapid development in theranostics, a variety of new targets are available for further clinical investigation.
Visualization and quantification of vesicle trafficking on a three‐dimensional cytoskeleton network in living cells (2007)

Summary Recent progress in biology and microscopy has made it possible to acquire multidimensional data on rapid cellular activities. Unfortunately, the data analysis needed to describe the observed biological process still remains a major bottleneck. We here present a novel method of studying membrane trafficking by monitoring vesicular structures moving along a three‐dimensional cytoskeleton network. It allows the dynamics of such structures to be qualitatively and quantitatively investigated. Our tracking method uses kymogram analysis to extract the consistent part of the temporal information and to allow the meaningful representation of vesicle dynamics. A fully automatic extension of this method, together with a statistical tool for dynamic comparisons, allows the precise analysis and comparison of overall speed distributions and directions. It can handle typical complex situations, such as a dense set of vesicles moving at various velocities, fusing and dissociating with each other or with other cell compartments. The overall approach has been characterized and validated on synthetic data. We chose the Rab6A protein, a GTPase involved in the regulation of intracellular membrane trafficking, as a molecular model. The application of our method to GFP‐Rab6A stable cells acquired using fast four‐dimensional deconvolution video‐microscopy gives considerable cellular dynamic information unreachable using other techniques.
Fumarase-catalyzed synthesis of L-threo-chloromalic acid and its conversion to 2-deoxy-D-ribose and D-erythro-sphingosine (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFumarase-catalyzed synthesis of L-threo-chloromalic acid and its conversion to 2-deoxy-D-ribose and D-erythro-sphingosineMark A. Findeis and George M. WhitesidesCite this: J. Org. Chem. 1987, 52, 13, 2838–2848Publication Date (Print):June 1, 1987Publication History Published online1 May 2002Published inissue 1 June 1987https://pubs.acs.org/doi/10.1021/jo00389a035https://doi.org/10.1021/jo00389a035research-articleACS PublicationsRequest reuse permissionsArticle Views362Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Prevalence of Neutralizing Antibodies to Adenoviral Serotypes 5 and 35 in the Adult Populations of The Gambia, South Africa, and the United States (2004)

One of the major limitations of the use of adenoviruses as gene therapy vectors is the existence of preformed immunity in various populations. Recent studies have linked failure of adenoviral gene therapy trials to the presence of antiadenoviral neutralizing antibodies (NAb). Understanding the distribution and specificity of such antibodies will assist in the design of successful recombinant adenoviral gene therapies and vaccines. To assess the prevalence of NAb to adenovirus serotypes 5 and 35 (Ad5 and Ad35), we analyzed serum samples from adult immunocompetent individuals living in The Gambia, South Africa, and the United States by using a neutralization assay. Serum samples were incubated with A549 lung carcinoma cells and adenoviruses encoding enhanced green or yellow fluorescent proteins; results were analyzed by fluorescence microscopy and flow cytometry. Using this technique, we found a high prevalence of NAb against Ad5 in Gambian, South African, and U.S. subjects at both low and high titers. Conversely, all subjects displayed a low prevalence of NAb to Ad35; when present, anti-Ad35 NAb were seen at low titers. Because of the ability of adenoviruses to elicit systemic and mucosal immune responses, Ad35 with its low NAb prevalence appears to be an attractive candidate vector for gene therapy applications.
Carrier capture processes in GaAs-AlGaAs quantum wells due to emission of confined phonons (1993)

Carrier capture times are calculated for GaAs-AlxGa1−xAs multiple quantum wells due to the emission of confined longitudinal optical phonons via electron-phonon (Fröhlich) interaction. We compare our results with several capture times measured by optical techniques and we discuss the importance of a knowledge of the carrier excitation details in order to obtain an appropriate interpretation of the experimental results. We show that electrons excited into confined states with a large kinetic energy (which have been ignored in previous works) strongly influence the overall capture times.
Genetic mutations in surfactant protein C are a rare cause of sporadic cases of IPF (2004)

Mutations in SFTPC are identified infrequently in this patient population. These findings indicate that SFTPC mutations do not contribute to the pathogenesis of IPF in the majority of sporadic cases.