gebhart/patrepeval huggingface dataset:
Avances doctrinales y jurisprudenciales de los supuestos de procedencia de la pretensión de carencia o abstención en Venezuela (2003)

Various properties of glucoamylase immobilized onto corn stover supporting material and separation of immobilized enzyme by tangential flow filtration unit were studied. Optimum pH and temperature of immobilized enzyme were 3.5 and 60 degrees C, respectively. Enzyme stability was studied in a packed-bed column. The starch conversion rate was attained at 81% for 15 days; after that, the hydrolysis rate gradually decreased. Size of supporting material proved to be an important factor, with higher activity and good loading yield resulting from smaller supporting material. Glucoamylase immobilized onto supporting material less than 44 microm was used for hydrolysis of 10% soluble starch at pH 3.5 and 40 degrees C for 3 h. Then immobilized glucoamylase was separated from the product by means of a tangential flow filtration unit using a 0.2-microm pore size Nylon 66 membrane filter. This operation was continued until 180 ml filtrate was obtained from a 260-mL starting volume. Then, the next batch was started by adding 180 mL starch substrate into the reactor. The batchwise experiments were repeated 20 times. The average filtration rate of each batch was determined and found to sharply decline during the first four batches. Thereafter, it gradually decreased from batch to batch. The cause of decreasing filtration rate appeared to be due to retrogradation of starch. The percentage of starch hydrolysis within 20 batches was in the range 89-96%. The filtration rate becomes higher if the hydrolyzation time is extended to 14 h. Resistance to filtration was also investigated. Almost all of the total resistance is related to insoluble materials, with the significant part of this from the resistance due to insoluble materials deposited on a surface of membrane and boundary layer resistance. Using a microscopic method, no microorganisms were found in the filtrate.
Lymphocyte programmed cell death is mediated via HLA class II DR (1994)

HLA class II molecules are constitutively expressed on human B lymphocytes and are induced on human T lymphocytes after activation, through which signal transduction via these molecules has been extensively described. We have observed cell death of as many as 60% after stimulation of lymphocytes via HLA class II DR molecules, but not via DP or DQ. Propidium iodide fluorescence, DNA fragmentation and morphology of the dead cells were examined. The reported cell death was very rapid, and independent of Fc receptors and of complement. A morphologically distinct sub-population of activated B cells was sensitive to HLA-DR mediated death, while resting B lymphocytes from the same donor did not die as a result of HLA class II mediated stimulation. Death via HLA-DR was distinguishable from necrosis. Cytoskeletal integrity, serine/threonine phosphatase activity and endonucleases were required for the pathway leading to HLA-DR mediated death. The 'ladder' pattern of DNA fragmentation which typically characterizes apoptosis was not observed, despite the observation of cell and nuclear shrinkage normally associated with apoptosis. These data suggest that HLA class II mediated death is a means of rapidly removing either T or B lymphocytes which have already served their role in the immune response, thereby avoiding the inflammatory responses associated with necrosis and concentrating the ligands for new TCR and/or CD4 interactions.
An Observer of Tire–Road Forces and Friction for Active Security Vehicle Systems (2007)

The estimation of vehicle dynamic variables is essential for the enhancement of safety, in particular for braking and trajectory-control systems. This paper proposes a new estimation process to calculate lateral tire forces, vehicle sideslip angle, and road friction. The estimation process (an adaptive observer) is constructed by combining a vehicle model and a tire force model. More specifically, this study proposes an adaptive tire force model that takes variations in road friction into account. The adaptive observer is evaluated in comparison with two nonadaptive observers that use tire force models with fixed parameters. This study also proposes a road friction identification method operating in an online context. The observers and the road friction identification method are first evaluated using vehicle simulator software. Subsequently, observers are compared to real data acquired using an experimental vehicle. This paper also includes a description of the experimental platform. Results show the accuracy and potential of the estimation process.
Multi-Label Zero-Shot Learning via Concept Embedding (2016)

Zero Shot Learning (ZSL) enables a learning model to classify instances of an unseen class during training. While most research in ZSL focuses on single-label classification, few studies have been done in multi-label ZSL, where an instance is associated with a set of labels simultaneously, due to the difficulty in modeling complex semantics conveyed by a set of labels. In this paper, we propose a novel approach to multi-label ZSL via concept embedding learned from collections of public users' annotations of multimedia. Thanks to concept embedding, multi-label ZSL can be done by efficiently mapping an instance input features onto the concept embedding space in a similar manner used in single-label ZSL. Moreover, our semantic learning model is capable of embedding an out-of-vocabulary label by inferring its meaning from its co-occurring labels. Thus, our approach allows both seen and unseen labels during the concept embedding learning to be used in the aforementioned instance mapping, which makes multi-label ZSL more flexible and suitable for real applications. Experimental results of multi-label ZSL on images and music tracks suggest that our approach outperforms a state-of-the-art multi-label ZSL model and can deal with a scenario involving out-of-vocabulary labels without re-training the semantics learning model.
Neural mediation of the motilin motor effect on the human antrum (1997)

To elucidate the mode of action of motilin on the stimulation of human gastrointestinal motility, we studied the effect of exogenous motilin during muscarinic or serotoninergic pharmacological blockade. Manometric recording of the interdigestive antroduodenal motility was carried out in 27 healthy volunteers until the appearance of a spontaneous antral phase III. The tested blocker was then administered intravenously and was followed 30 min later by a 10-min infusion of synthetic human motilin (50 ng/kg). Motilin administered on a background of saline induced a premature phase III migrating from the antrum to the duodenum in every tested subject (n = 5). A low dose of atropine (5 micrograms.kg-1.h-1 for 90 min) inhibited the motilin effect in two of five subjects [not significant (NS)], whereas a high dose of atropine (15 micrograms/kg given in 30 min) blocked the motilin-induced premature antral phase III in all instances (n = 5, P < 0.01). Exogenous motilin given with low-dose ondanseton (8 mg given in 15 min followed by 1 mg/h for 90 min) or high-dose ondansetron (32 mg given in 30 min) was without effect in three of seven (NS) or in two of five (NS) subjects, respectively. During the administration of 15 micrograms/kg atropine, when exogenous motilin always failed to induce a premature antral phase III motor, a phase III-type activity was generated at the duodenum in four of five subjects. We conclude that the induction by motilin of phase III activity in human antrum is dependent on muscarinic mediation and that the contractile effect of motilin on human duodenum involves a noncholinergic mechanism, different therefore from the antral pathway.
Antiestrogens. Synthesis and evaluation of mammary tumor inhibiting activity of 1,1,2,2-tetraalkyl-1,2-diphenylethanes (1980)

Among the newly synthesized 1,1,2,2-tetraalkyl-1,2-diphenylethanes, 1,1,2,2-tetramethyl-1,2-bis(4'-hydroxyphenyl)ethane (23) and 1,1,2,2-tetramethyl-1,2-bis(3'-hydroxyphenyl)ethane (26) were the most active compounds regarding estradiol receptor affinity, exhibiting Ka values of 0.73 X 10(8) and 0.67 X 10(8) M-1, respectively. In vivo, 23 and 26 showed only very small uterotrophic activity in the mouse. They strongly inhibited (73%) the estrone-stimulated mouse uterine growth. Tested on the 9,10-dimethyl-1,2-benzanthracene induced hormone-dependent mammary adenocarcinoma of the Sprague-Dawley rat, compounds 23 and 26 exhibited a dose-dependent inhibition of the tumor growth, having a strong effect at a dose of 20 (mg/kg)/day (compound 23).
Demonstration of a coherent PMD source (2003)

A coherent programmable polarization-mode-dispersion (PMD) source is demonstrated. Measured PMD spectra are favorably compared with coherent PMD theory. Completely predictable PMD sources are essential to rigorously test receivers and transport systems against PMD impairment. PMD sources using coherent PMD technology can generate predictable PMD that includes differential group delay (DGD), second-order PMD (SOPMD), and polarization-dependent chromatic dispersion (PDCD). The emphasis in this work is on harmonic and coherent spectra.
Therapeutic uses of rTMS (2003)

The basic rationale for attempting to use rTMS as a therapeutic tool is that it is known to produce effects on cerebral cortex that outlast the stimulus. The assumption is that, in some cases, it may be possible to manipulate these long-term effects either to reverse the pathological processes responsible for the condition, or to change the excitability of remaining healthy systems so that they can compensate for the underlying disturbance. In this chapter we will consider the use of rTMS in psychiatric conditions and in movement disorders. However, before discussing clinical details, we consider the available data about the long-term effects of rTMS in healthy subjects from the standpoint of designing therapeutic trials on patients. In particular, we ask first whether rTMS can ever be targeted accurately enough at specific neural populations to achieve a therapeutic effect and, second, whether the effects it produces will last long enough to be used as a clinical treatment.
Flocculation, adhesion and biofilm formation in yeasts (2006)

Yeast cells possess a remarkable capacity to adhere to abiotic surfaces, cells and tissues. These adhesion properties are of medical and industrial relevance. Pathogenic yeasts such as Candida albicans and Candida glabrata adhere to medical devices and form drug-resistant biofilms. In contrast, cell-cell adhesion (flocculation) is a desirable property of industrial Saccharomyces cerevisiae strains that allows the easy separation of cells from the fermentation product. Adhesion is conferred by a class of special cell wall proteins, called adhesins. Cells carry several different adhesins, each allowing adhesion to specific substrates. Several signalling cascades including the Ras/cAMP/PKA and MAP kinase (MAPK)-dependent filamentous growth pathways tightly control synthesis of the different adhesins. Together, these pathways trigger adhesion in response to stress, nutrient limitation or small molecules produced by the host, such as auxin in plants or NAD in mammals. In addition, adhesins are subject to subtelomeric epigenetic switching, resulting in stochastic expression patterns. Internal tandem repeats within adhesin genes trigger recombination events and the formation of novel adhesins, thereby offering fungi an endless reservoir of adhesion properties. These aspects of fungal adhesion exemplify the impressive phenotypic plasticity of yeasts, allowing them to adapt quickly to stressful environments and exploit new opportunities.
The nonantimicrobial properties of tetracycline for the treatment of periodontal disease. (1994)

Tetracyclines have nonantimicrobial properties that appear to modulate host response. In that regard, tetracyclines and their nonantimicrobial chemically modified analogues (chemically modified tetracycline molecules [CMTs]) inhibit the extracellular activity of mammalian neutrophil and osteoblast collagenases. The activity of this matrix metalloproteinase appears crucial in the destruction of collagen. Apart from its anticollagenase effect, tetracyclines are also potent inhibitors of osteoclast function. Several recent studies have also addressed the therapeutic potential of tetracyclines and CMTs in periodontal disease. These drugs reduced excessive gingival collagenase activity and severity of periodontal breakdown in rats infected with Porphyromonas gingivalis and in diabetic rats. CMT was not associated with the emergence of resistant microorganisms. In human double-blind clinical trials, low-dose doxycycline therapy substantially reduced collagenase activity in the gingival and crevicular fluid, and prevented the loss of attachment in adult periodontitis without the emergence of doxycycline-resistant microorganisms. Tetracyclines and CMTs have enormous therapeutic potential because these drugs can inhibit the activity of matrix metalloproteinases as well as osteoclast function, and thus prevent the degradation of osseous connective tissues in periodontal as well as arthritic diseases.
Schaum's outline of theory and problems of graph theory (1997)

The Sum Rule and the Product Rule. -- Permutations and Combinations. -- The Pigeonhole Principle. -- Generalized Permutations and Combinations. -- Sequences and Selections. -- The Inclusion-Exclusion Principle. -- Generating Functions and Partitions of Integers. -- The Distribution Problem in Combinatorics. -- Recurrence Relations. -- Group Theory in Combinatorics -- Including The Burnside-Froberius Theorem. -- Permutation Groups and Their Cycles Indices and Polya's Enumeration Theorems.
KCNC3: phenotype, mutations, channel biophysics-a study of 260 familial ataxia patients (2009)

We recently identified KCNC3, encoding the Kv3.3 voltage-gated potassium channel, as the gene mutated in SCA13. One g.10684G>A (p.Arg420His) mutation caused late-onset ataxia resulting in a nonfunctional channel subunit with dominant-negative properties. A French early-onset pedigree with mild mental retardation segregated a g.10767T>C (p.Phe448Leu) mutation. This mutation changed the relative stability of the channel's open conformation. Coding exons were amplified and sequenced in 260 autosomal-dominant ataxia index cases of European descent. Functional analyses were performed using expression in Xenopus oocytes. The previously identified p.Arg420His mutation occurred in three families with late-onset ataxia. A novel mutation g.10693G>A (p.Arg423His) was identified in two families with early-onset. In one pedigree, a novel g.10522G>A (p.Arg366His) sequence variant was seen in one index case but did not segregate with affected status in the respective family. In a heterologous expression system, the p.Arg423His mutation exhibited dominant-negative properties. The p.Arg420His mutation, which results in a nonfunctional channel subunit, was recurrent and associated with late-onset progressive ataxia. In two families the p.Arg423His mutation was associated with early-onset slow-progressive ataxia. Despite a phenotype reminiscent of the p.Phe448Leu mutation, segregating in a large early-onset French pedigree, the p.Arg423His mutation resulted in a nonfunctional subunit with a strong dominant-negative effect.
Nucleotide sequence between thefadB gene and therrnA operon fromEscherichia coli (1990)

Journal Article Nucleotide sequence between the fad B gene and the rrn A operon from Escherichia coli Get access Kenji Nakahigashi, Kenji Nakahigashi Department of Biophysics, Faculty of Science, Kyoto UniversitySakyo-ku, Kyoto 606, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Hachiro Inokuchi Hachiro Inokuchi Department of Biophysics, Faculty of Science, Kyoto UniversitySakyo-ku, Kyoto 606, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 21, 21 November 1990, Page 6439, https://doi.org/10.1093/nar/18.21.6439 Published: 21 November 1990 Article history Received: 24 September 1990 Published: 21 November 1990
Inferring Reasons for the Failure of Staphylococcus aureus Vaccines in Clinical Trials (2012)

OPINION article Front. Cell. Infect. Microbiol., 22 February 2012Sec. Clinical Microbiology Volume 2 - 2012 | https://doi.org/10.3389/fcimb.2012.00016
Cardiac fibroblasts are predisposed to convert into myocyte phenotype: specific effect of transforming growth factor beta. (1991)

Cardiac fibroblasts are mainly responsible for the synthesis of major extracellular matrix proteins in the heart, including fibrillar collagen types I and III and fibronectin. In this report we show that these cells, when stimulated by transforming growth factor beta 1 (TGF-beta 1), acquire certain myocyte-specific properties. Cultured cardiac fibroblasts from adult rabbit heart were treated with TGF-beta 1 (10-15 ng/ml) for different periods of time. Northern hybridization analysis of total RNA showed that cells treated with TGF-beta 1 for 24 hr expressed mRNA corresponding to sarcomeric actin mRNA. Immunofluorescence staining and light microscopy showed that cultured cardiac fibroblasts treated with TGF-beta 1 became stained with a monoclonal antibody to muscle-specific actin. After treatment of quiescent cells with TGF-beta 1, cell proliferation (as measured by [3H]thymidine incorporation) was moderately increased (1.5-fold, P less than 0.001). NIH 3T3 cells and human skin fibroblasts, treated with TGF-beta 1, did not express sarcomeric actin mRNA. Treatment of cardiac fibroblasts with the mitogenic agent phorbol 12-myristate 13-acetate or with norepinephrine, angiotensin II, or interleukin 1 beta did not induce myocyte-specific actin mRNA. Cultured cardiac fibroblasts at the subconfluent stage, when exposed to TGF-beta 1 in the presence of 10% fetal bovine serum, gave rise to a second generation of slowly growing cells that expressed muscle-specific actin filaments. Our findings demonstrate that cardiac fibroblasts can be made to differentiate into cells that display many characteristics of cardiac myocytes. TGF-beta 1 seems to be a specific inducer of such conversion.
The significance of limulus amebocyte lysate test specificity on the pyrogen evaluation of parenteral drugs. (1980)

Although the sensitivity of the Limulus Amebocyte Lysate (LAL) Test for gram-negative endotoxin is well established, several reports have appeared in the literature indicating that substances other than endotoxin can elicit positive LAL test reactions under certain conditions. This discussion of LAL test specificity deals with the impact these findings could have on the use of LAL for pyrogen testing in the parenteral drug industry, and concludes that false positive LAL test reactions due to substances other than endotoxin need not concern most parenteral drug manufacturers. These, however, can be ruled out by using appropriate controls.
Protection against Mucosal Simian Immunodeficiency Virus SIV mac251 Challenge by Using Replicating Adenovirus-SIV Multigene Vaccine Priming and Subunit Boosting (2004)

Whereas several recent AIDS vaccine strategies have protected rhesus macaques against a pathogenic simian/human immunodeficiency virus (SHIV)(89.6P) challenge, similar approaches have provided only modest, transient reductions in viral burden after challenge with virulent, pathogenic SIV, which is more representative of HIV infection of people. We show here that priming with replicating adenovirus recombinants encoding SIV env/rev, gag, and/or nef genes, followed by boosting with SIV gp120 or an SIV polypeptide mimicking the CD4 binding region of the envelope, protects rhesus macaques from intrarectal infection with the highly pathogenic SIV(mac251). Using trend analysis, significant reductions in acute-phase and set point viremia were correlated with anti-gp120 antibody and cellular immune responses, respectively. Within immunization groups exhibiting significant protection, a subset (39%) of macaques have exhibited either no viremia, cleared viremia, or controlled viremia at the threshold of detection, now more than 40 weeks postchallenge. This combination prime-boost strategy, utilizing replication competent adenovirus, is a promising alternative for HIV vaccine development.
Presentation secrets of Steve Jobs how to be insanely great in front of any audience (2010)

Presentation Secrets of Steve Jobs reveals the operating system behind any presentation and provides you with a quick-start guide to design your own passionate interfaces with your audiences. Cliff Atkinson, author of Beyond Bullet Points and The Activist Audience Apple CEO Steve Jobs's wildly popular presentations have set a new global gold standard--and now this step-by-step guide shows you exactly how to use his crowd-pleasing techniques in your own presentations. The Presentation Secrets of Steve Jobs is as close as you'll ever get to having the master presenter himself speak directly in your ear. Communications expert Carmine Gallo has studied and analyzed the very best of Jobs's performances, offering point-by-point examples, tried-and-true techniques, and proven presentation secrets that work every time. With this revolutionary approach, you'll be surprised at how easy it is to sell your ideas, share your enthusiasm, and wow your audience the Steve Jobs way. Watch Carmine Gallo describe how you can inspire, motivate, and lead your team to do insanely great things. other leader captures an audience like Steve Jobs does and, like no other book, The Presentation Secrets of Steve Jobs captures the formula Steve uses to enthrall audiences. Rob Enderle, The Enderle Group Now you can learn from the best there is both Jobs and Gallo. No matter whether you are a novice presenter or a professional speaker like me, you will read and reread this book with the same enthusiasm that people bring to their iPods. David Meerman Scott, bestselling author of The New Rules of Marketing & PR and World Wide Rave
Corneal Epithelial Wound Healing (2001)

One of the important functions of the cornea is to maintain normal vision by refracting light onto the lens and retina. This property is dependent in part on the ability of the corneal epithelium to undergo continuous renewal. Epithelial renewal is essential because it enables this tissue to act as a barrier that protects the corneal interior from becoming infected by noxious environmental agents. Furthermore, the smooth optical properties of the corneal epithelial surface are sustained through this renewal process. The rate of renewal is dependent on a highly integrated balance between the processes of corneal epithelial proliferation, differentiation, and cell death. One experimental approach to characterize these three aspects of the renewal process has been to study the kinetics and dynamics of corneal re-epithelialization in a wound-healing model. This effort has employed in vivo and in vitro studies. From such studies it is evident that the appropriate integration and coordination of corneal epithelial proliferation, adhesion, migration, and cell demise is dependent on the actions of a myriad of cytokines. Our goal here is to provide an overview into how these mediators and environmental factors elicit control of cellular proliferation, adhesion, migration, and apoptosis. To this end we review the pertinent literature dealing with the receptor and the cell signaling events that are responsible for mediating cytokine control of corneal epithelial renewal. It is our hope that a better appreciation can be obtained about the complexity of the control processes that are responsible for assuring continuous corneal epithelial renewal in health and disease.