gebhart/patrepeval huggingface dataset:
Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene (1989)

A gene bank from the chlorinated hydrocarbon-degrading bacterium Xanthobacter autotrophicus GJ10 was prepared in the broad-host-range cosmid vector pLAFR1. By using mutants impaired in dichloroethane utilization and strains lacking dehalogenase activities, several genes involved in 1,2-dichloroethane metabolism were isolated. The haloalkane dehalogenase gene dhlA was subcloned, and it was efficiently expressed from its own constitutive promoter in strains of a Pseudomonas sp., Escherichia coli, and a Xanthobacter sp. at levels up to 30% of the total soluble cellular protein. A 3-kilobase-pair BamHI DNA fragment on which the dhlA gene is localized was sequenced. The haloalkane dehalogenase gene was identified by the known N-terminal amino acid sequence of its product and found to encode a 310-amino-acid protein of molecular weight 35,143. Upstream of the dehalogenase gene, a good ribosome-binding site and two consensus E. coli promoter sequences were present.
Wavelet-based Rician noise removal for magnetic resonance imaging (1999)

It is well known that magnetic resonance magnitude image data obey a Rician distribution. Unlike additive Gaussian noise, Rician "noise" is signal-dependent, and separating signal from noise is a difficult task. Rician noise is especially problematic in low signal-to-noise ratio (SNR) regimes where it not only causes random fluctuations, but also introduces a signal-dependent bias to the data that reduces image contrast. This paper studies wavelet-domain filtering methods for Rician noise removal. We present a novel wavelet-domain filter that adapts to variations in both the signal and the noise.
Isotachophoresis in Free-Flow Using a Miniaturized Device (2006)

For the first time, we report a miniaturized approach for isotachophoresis employing the technique of free-flow electrophoresis. Using a micromachined separation chamber with a volume of 200 nL, a sample mixture of fluorescein, eosin G, and acetylsalicylic acid was separated, stacked, and concentrated in less than a minute. Additionally, an isotachophoretic separation of a reaction mixture of myoglobin and fluoresceinisothiocyanate as a fluorescence label has shown the potential of this method for on-line sample preparation.
Laser Surface Scanning for Patient Registration in Intracranial Image-guided Surgery (2002)

Laser scanning for surface registration is an accurate, robust, and easy-to-use method of patient registration for image-guided surgery.
Elevated Cytokines, Thrombin and PAI-1 in Severe HCPS Patients Due to Sin Nombre Virus (2015)

Sin Nombre Hantavirus (SNV, Bunyaviridae Hantavirus) is a Category A pathogen that causes Hantavirus Cardiopulmonary Syndrome (HCPS) with case fatality ratios generally ranging from 30% to 50%. HCPS is characterized by vascular leakage due to dysregulation of the endothelial barrier function. The loss of vascular integrity results in non-cardiogenic pulmonary edema, shock, multi-organ failure and death. Using Electric Cell-substrate Impedance Sensing (ECIS) measurements, we found that plasma samples drawn from University of New Mexico Hospital patients with serologically-confirmed HCPS, induce loss of cell-cell adhesion in confluent epithelial and endothelial cell monolayers grown in ECIS cultureware. We show that the loss of cell-cell adhesion is sensitive to both thrombin and plasmin inhibitors in mild cases, and to thrombin only inhibition in severe cases, suggesting an increasing prothrombotic state with disease severity. A proteomic profile (2D gel electrophoresis and mass spectrometry) of HCPS plasma samples in our cohort revealed robust antifibrinolytic activity among terminal case patients. The prothrombotic activity is highlighted by acute ≥30 to >100 fold increases in active plasminogen activator inhibitor (PAI-1) which, preceded death of the subjects within 48 h. Taken together, this suggests that PAI-1 might be a response to the severe pathology as it is expected to reduce plasmin activity and possibly thrombin activity in the terminal patients.
New applications of disease genetics and pharmacogenetics to drug development (2014)

TOMMORROW is a Phase III delay of onset clinical trial to determine whether low doses of pioglitazone, a molecule that induces mitochondrial doubling, delays the onset of MCI-AD in normal subjects treated with low dose compared to placebo. BOLD imaging studies in rodents and man were used to find the dose that increases oxygen consumption at central regions of the brain in higher proportion than activation of large corticol regions. The trial is made practical by the use of a pharmacogenetic algorithm based on TOMM40 and APOE genotypes and age to identify normal subjects at high risk of MCI-AD between the ages of 65–83 years within a five year follow-up period.
Injectable Self-Assembling Peptide Nanofibers Create Intramyocardial Microenvironments for Endothelial Cells (2005)

These experiments demonstrate that self-assembling peptides can create nanofiber microenvironments in the myocardium and that these microenvironments promote vascular cell recruitment. Because these peptide nanofibers may be modified in a variety of ways, this approach may enable injectable tissue regeneration strategies.
Synthesis and evaluation of new ditopic cyclophane receptors for benzoic acid (2005)

Abstract As potential receptors for a benzoic acid guest, new ditopic cyclophane host molecules, each with a π electron‐rich portion and a pyridine ring‐containing portion, are synthesized. Complexation of benzoic acid guest by the potential molecular receptors is probed by infrared spectroscopy, proton magnetic resonance titration experiments and solid‐state structure determination. Copyright © 2005 John Wiley &amp; Sons, Ltd.
The role of nitric oxide synthase in endotoxin-induced uveitis: effects of NG-nitro L-arginine. (1994)

Induction of iNOS may play a key role in the pathogenesis of EIU. Because inhibition of iNOS activity reduced the inflammatory response, suppression of NO formation may inhibit the development of EIU.
<title>Removal of defects on flash radiographic images by fuzzy combination</title> (1995)

We present a method for automatic detection and removal of defects on flash X-ray images. In our experiments, we obtain about ten images representing the radiographic projection of the same object. Defects appear on some images and limit the measurement accuracy. Our aim is to assess the radiant image (image before detection) by combining the relevant information on the different radiographs. First, we isolate the defects from the object projection by the point by point difference between two images. Then, a multiscale filtering is performed on the difference images to extract the defects according to their spatial extent. Next, each filtered image is segmented by a fuzzy clustering method which takes into account inaccurate edges of the defects. The resultant fuzzy images indicate the defect importance on the difference images by a degree between 0 and 1. Afterwards, the fuzzy images obtained are aggregated to retrieve the fuzzy relevance degrees of the measurement on each original image. Finally, the defects are removed by a fuzzy combination of the images according to their relevance degrees. On the resultant images, the defects are well suppressed thanks to the selection of the reliable information.
Pudim de mel aromatizado com capim-santo (2018)

O pudim e a sobremesa mais popular das mesas brasileiras, e uma receita de origem portuguesa, com o tempo a popularidade do pudim se expandiu, agradando o paladar de diversos povos e geracoes, seu sucesso pode ser explicado por seu sabor delicioso e tambem por ser um doce muito facil e rapido de se fazer e sempre com formulacoes novas. Os alimentos funcionais possuem potencial para promover a saude por meio de mecanismos nao previstos pela nutricao convencional, devendo ser salientado que esse efeito restringe-se a promocao da saude e nao a cura de doencas. A busca por produtos naturais e saudaveis cresceu muito, motivo pelo qual o consumo de mel tem aumentado nos ultimos anos. Mel e um produto rico em acucares – o nectar original possui ate 87% onde predominam glicose e frutose e que possui tambem, em quantidades muito menores, aminoacidos, minerais, acidos orgânicos, enzimas, entre outros. O capim-santo tem como composicao o limoneno, citronelal, mirceno, geraniol e o seu principal o citral, tambem tem efeitos terapeuticos, e famosos pelos seus efeitos antibacterianos e calmantes, comprovados cientificamente. Diante deste fato, o presente trabalho teve como objetivo elaborar e avaliar a aceitacao sensorial do pudim com sabor de mel e aromatizado por capim-santo, dois alimentos com um alto valor nutritivo e terapeutico. O trabalho foi desenvolvido no laboratorio de analise sensorial da Universidade Federal de Campina Grande. Foi utilizado para a fabricacao do pudim de mel aromatizado com capim-santo: 395g de leite condensado, 200 ml de leite, 100g de capim santo e 21g de mel. O teste de aceitacao sensorial contou com a presenca de 70 provadores nao treinados, com faixa etaria de 17 a 35 anos, que receberam uma ficha resposta contendo uma escala hedonica estruturada de 9 pontos para avaliar seu julgamento em relacao a aceitacao do pudim. Os atributos avaliados foram: aroma, cor, sabor, textura, docura e aparencia global apresentaram um bom indice de aceitacao, considerando que ao se desenvolver um novo produto, um dos pontos fundamentais e avaliar sua aceitabilidade, a fim de predizer seu comportamento frente ao mercado consumidor, a adicao de mel nas quantidades testadas nao modificou a formulacao do pudim. Desta forma, o pudim agradou o publico avaliador, obtendo assim uma excelente intencao de compra, demonstrando que a formulacao obteve sucesso em seus resultados e o objetivo do trabalho foi alcancado.
Switching the light emission of (4-biphenylyl)phenyldibenzofulvene by morphological modulation: crystallization-induced emission enhancement (2006)

(4-Biphenylyl)phenyldibenzofulvene is weakly luminescent in the amorphous phase but becomes highly emissive upon crystallization; this unusual crystallization-induced emission enhancement effect allows its emission to be repeatedly switched between dark and bright states by fuming-heating and heating-cooling processes.
Microbial Decontamination of Parathion and p -Nitrophenol in Aqueous Media (1974)

A mixed microbial culture was adapted to growth on parathion to determine the feasibility of using microorganisms to detoxify concentrated parathion in agricultural wastes. In a 600-ml chemostat, the culture was able to degrade 50 mg of parathion per liter per h. Para-nitrophenol, produced by enzymatic hydrolysis of parathion, caused delays in exponential growth which were directly proportional to its concentration. A pseudomonad, isolated from the mixed culture, exhibited optimal growth at 0.21 mM p-nitrophenol and grew in concentrations up to 3.5 mM. In metabolic studies using [(14)C]p-nitrophenol, the nitro group was removed in stoichiometric quantities as nitrite and hydroquinone was tentatively identified as a metabolite.
An Expedient Route for the Stereoselective Construction of Bridged Polyheterocyclic Ring Systems Using the Tandem “Pincer” Diels−Alder Reaction (1997)

The tandem "pincer" Diels-Alder reaction, consisting of two consecutive [4 + 2] cycloadditions between two dienes and an acetylenic bis-dienophile, has been applied toward the rapid construction of bridged polyoxacyclic ring systems when furan derivatives are used as the diene components. The study has demonstrated the stereoselectivity (exo-exo adduct), the chemoselectivity ("pincer" vs "domino"), as well as the regioselectivity of the reaction. The reaction has been successfully applied to a variety of 2-substituted furans and tethered bis-furans in combination with monoactivated and diactivated dienophiles. The synthesis of unsymmetrical cycloadducts starting from the aza- and oxanorbornadiene-type intermediate has also been realized.
Vertically aligned carbon nanofibers and related structures: Controlled synthesis and directed assembly (2005)

The controlled synthesis of materials by methods that permit their assembly into functional nanoscale structures lies at the crux of the emerging field of nanotechnology. Although only one of several materials families is of interest, carbon-based nanostructured materials continue to attract a disproportionate share of research effort, in part because of their wide-ranging properties. Additionally, developments of the past decade in the controlled synthesis of carbon nanotubes and nanofibers have opened additional possibilities for their use as functional elements in numerous applications. Vertically aligned carbon nanofibers (VACNFs) are a subclass of carbon nanostructured materials that can be produced with a high degree of control using catalytic plasma-enhanced chemical-vapor deposition (C-PECVD). Using C-PECVD the location, diameter, length, shape, chemical composition, and orientation can be controlled during VACNF synthesis. Here we review the CVD and PECVD systems, growth control mechanisms, catalyst preparation, resultant carbon nanostructures, and VACNF properties. This is followed by a review of many of the application areas for carbon nanotubes and nanofibers including electron field-emission sources, electrochemical probes, functionalized sensor elements, scanning probe microscopy tips, nanoelectromechanical systems (NEMS), hydrogen and charge storage, and catalyst support. We end by noting gaps in the understanding of VACNF growth mechanisms and the challenges remaining in the development of methods for an even more comprehensive control of the carbon nanofiber synthesis process.
Vaccines for Mucosal Immunity to Combat Emerging Infectious Diseases (2000)

The mucosal immune system consists of molecules, cells, and organized lymphoid structures intended to provide immunity to pathogens that impinge upon mucosal surfaces. Mucosal infection by intracellular pathogens results in the induction of cell- mediated immunity, as manifested by CD4-positive (CD4 + ) T helper-type 1 cells, as well as CD8 + cytotoxic T-lymphocytes. These responses are normally accompanied by the synthesis of secretory immunoglobulin A (S-IgA) antibodies, which provide an important first line of defense against invasion of deeper tissues by these pathogens. New-generation live, attenuated viral vaccines, such as the cold-adapted, recombinant nasal influenza and oral rotavirus vaccines, optimize this form of mucosal immune protection. Despite these advances, new and reemerging infectious diseases are tipping the balance in favor of the parasite; continued mucosal vaccine development will be needed to effectively combat these new threats.
Frequency shifts of cantilevers vibrating in various media (1996)

A simple model is presented for rectangular cantilevers when vibrating in various media. The mass of the surrounding medium affected by the motion of the lever is calculated. It depends on the dimensions of the lever, on the excited mode, and on the density of the medium. Although the viscosity of the media is not taken into account, the resulting predictions for the resonance frequencies agree well with experimental data obtained for levers in air and water up to the seventh harmonic.
Phenotypic characterization of glucose repression mutants of Saccharomyces cerevisiae using experiments with 13C‐labelled glucose (2004)

In the field of metabolic engineering and functional genomics, methods for analysis of metabolic fluxes in the cell are attractive as they give an overview of the phenotypic response of the cells at the level of the active metabolic network. This is unlike several other high-throughput experimental techniques, which do not provide information about the integrated response a specific genetic modification has on the cellular function. In this study we have performed phenotypic characterization of several mutants of the yeast Saccharomyces cerevisiae through the use of experiments with (13)C-labelled glucose. Through GC-MS analysis of the (13)C incorporated into the amino acids of cellular proteins, it was possible to obtain quantitative information on the function of the central carbon metabolism in the different mutants. Traditionally, such labelling data have been used to quantify metabolic fluxes through the use of a suitable mathematical model, but here we show that the raw labelling data may also be used directly for phenotypic characterization of different mutant strains. Different glucose derepressed strains investigated employed are the disruption mutants reg1, hxk2, grr1, mig1 and mig1mig2 and the reference strain CEN.PK113-7D. Principal components analysis of the summed fractional labelling data show that deleting the genes HXK2 and GRR1 results in similar phenotype at the fluxome level, with a partial alleviation of glucose repression on the respiratory metabolism. Furthermore, deletion of the genes MIG1, MIG1/MIG2 and REG1 did not result in a significant change in the phenotype at the fluxome level.
Mössbauer and EPR studies of the binuclear iron center in ribonucleotide reductase from Escherichia coli (1989)

57Fe-enriched ribonucleotide reductase subunit B2 from Escherichia coli strain N6405/pSPS2 has been characterized by Mössbauer and EPR spectroscopy in its native diferric state and in a new differous form. The native protein exhibits two Mössbauer doublets in a 1:1 ratio with parameters that are in excellent agreement with those reported for the wild-type protein (Atkin, C. L., Thelander, L., Reichard, P., and Lang, G. (1983) J. Biol. Chem. 248, 7464-7472); in addition, our studies show the absence of adventitiously bound iron. The iron content in the present samples approached 4 per B2 subunit, and the tyrosyl radical content exceeded 1 per B2 subunit. The higher values are attributed to the use of a new epsilon 280 for the protein and more efficient methods for iron extraction. We thus propose that subunit B2 has two binuclear iron clusters, each associated with its own tyrosyl radical, in contradistinction from the prevailing model. Reduction of the native protein with dithionite or reconstitution of the apoprotein with Fe(II) afforded a protein complex with Mössbauer parameters, delta EQ = 3.13 mm/s and delta = 1.26 mm/s at 4.2 K, and a low field EPR signal associated with an integer spin system. These spectral properties resemble those of methane monooxygenase in its diferrous form. Upon exposure to O2, the reduced subunit B2 readily converts to the diferric state and yields active enzyme.
An object-based executable model for simulation of real-time Hw/Sw systems (1999)

This paper describes a simulation technique for real-time Hw/Sw systems based on an object executable model. It allows designers to seamlessly estimate and verify their solutions from a high-level functional description to a Hw/Sw partitioned design. The same model, enhanced with algorithms, can be used to simulate interpreted models in order to observe the behavior of a whole system and its environment, as well as uninterpreted models which are useful for performance estimation and so help Hw/Sw partitioning. Our (co-)simulation technique is based on the translation of a high level model of the application into a C++ program which uses a library of predefined classes. While running, the program produces an event trace, the contents of which can be set by the user. This technique allows us to quickly analyze the influence of application or architecture parameters on the application behavior.
Thromboxane-induced phosphatidate formation in human platelets. Relationship to receptor occupancy and to changes in cytosolic free calcium (1984)

The inter-relationships between receptor occupancy, inositol phospholipid metabolism and elevation of cytosolic free Ca2+ in thromboxane A2-induced human platelet activation were investigated by using the stable thromboxane A2 mimetic, 9,11-epoxymethanoprostaglandin H2, and the thromboxane A2 receptor antagonist, EPO45. 9,11-Epoxymethanoprostaglandin H2 stimulated platelet phosphatidylinositol metabolism as indicated by the rapid accumulation of [32P]phosphatidate and later accumulation of [32P]phosphatidylinositol in platelets pre-labelled with [32P]Pi. These effects of 9,11-epoxymethanoprostaglandin H2 were concentration-dependent and half-maximal [32P]phosphatidate formation occurred at an agonist concentration of 54 +/- 8 nM. With platelets labelled with the fluorescent Ca2+ indicator quin 2, resting cytosolic free Ca2+ was 86 +/- 12 nM. 9,11-Epoxymethanoprostaglandin H2 induced a rapid, concentration-dependent elevation of cytosolic free Ca2+ to a maximum of 300-700 nM. Half-maximal stimulation was observed at an agonist concentration of 80 +/- 23 nM. The thromboxane A2 receptor antagonist EPO45 selectively inhibited 9,11-epoxymethanoprostaglandin H2-induced [32P]phosphatidate formation and elevation of cytosolic free Ca2+, indicating that both events are sequelae of receptor occupancy. Human platelets contain a single class of stereospecific, saturable, high affinity (KD = 70 +/- 13 nM) binding sites for 9,11-epoxymethano[3H]prostaglandin H2. The concentration-response curve for receptor occupancy (9,11-epoxymethano-[3H]prostaglandin H2 binding) is similar to that for 9,11-epoxymethanoprostaglandin H2-induced [32P]phosphatidate formation and for elevation of cytosolic free Ca2+. These observations indicate that human platelet thromboxane A2 receptor occupation is closely linked to inositol phospholipid metabolism and to elevation of cytosolic free Ca2+. Both such events may be necessary for thromboxane A2-induced human platelet activation.
A SYNTHESIS OF TAURINE (1927)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA SYNTHESIS OF TAURINEC. S. Marvel, C. F. Bailey, and M. S. SparbergCite this: J. Am. Chem. Soc. 1927, 49, 7, 1833–1837Publication Date (Print):July 1, 1927Publication History Published online1 May 2002Published inissue 1 July 1927https://pubs.acs.org/doi/10.1021/ja01406a029https://doi.org/10.1021/ja01406a029research-articleACS PublicationsRequest reuse permissionsArticle Views723Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts