gebhart/patrepeval huggingface dataset:
Molecular cloning of two new heat shock genes related to the hsp70 genes in Staphylococcus aureus (1994)

We have identified two new heat shock protein genes, orf37 and orf35, in Staphylococcus aureus, located upstream and downstream of grpE(hsp20), dnaK(hsp70), and dnaJ(hsp40) homologous genes in the order orf37-hsp20-hsp70-hsp40-orf35. The transcripts of both orf37 and orf35 were increased by thermal upshift of the culture from 37 to 46 degrees C. The heat shock promoters were located upstream of orf37 and upstream of hsp40. The deduced peptide of orf37 showed similarity with those of orfA in Clostridium acetobutylicum and orf39 in Bacillus subtilis. orf35 was unique in S. aureus and has not yet been described in other bacteria.
Automation of biochip array technology for quality results (2005)

Control processes employed in the manufacture and analysis of Evidence components ensure reproducibility of assays for a range of routine and novel markers.
Providing resiliency to load variations in distributed stream processing (2006)

Scalability in stream processing systems can be achieved by using a cluster of computing devices. The processing burden can, thus, be distributed among the nodes by partitioning the query graph. The specific operator placement plan can have a huge impact on performance. Previous work has focused on how to move query operators dynamically in reaction to load changes in order to keep the load balanced. Operator movement is too expensive to alleviate short-term bursts; moreover, some systems do not support the ability to move operators dynamically. In this paper, we develop algorithms for selecting an operator placement plan that is resilient to changes in load. In other words, we assume that operators cannot move, therefore, we try to place them in such a way that the resulting system will be able to withstand the largest set of input rate combinations. We call this a resilient placement.This paper first formalizes the problem for operators that exhibit linear load characteristics (e.g., filter, aggregate), and introduces a resilient placement algorithm. We then show how we can extend our algorithm to take advantage of additional workload information (such as known minimum input stream rates). We further show how this approach can be extended to operators that exhibit non-linear load characteristics (e.g., join). Finally, we present prototype- and simulation-based experiments that quantify the benefits of our approach over existing techniques using real network traffic traces.
Enhanced Fc-Dependent Cellular Cytotoxicity of Fc Fusion Proteins Derived from TNF Receptor II and LFA-3 by Fucose Removal from Asn-Linked Oligosaccharides (2006)

Fucose removal from complex-type oligosaccharide of human IgGs results in a major enhancement of Fc-dependent cellular cytotoxicity. The aim of this study was to determine the effect of fucose removal on the effector function of another class of clinically important molecules that can effect cellular cytotoxicity, Fc fusion proteins. The receptors chosen for study were TNF receptor II and LFA-3, both of which have therapeutic significance. The fucosylated versions of these fusion proteins were produced in unmodified CHO cells, whereas the nonfucosylated counterparts were produced in CHO cells with alpha-1,6-fucosyltransferase, an enzyme required for fucosylation, knocked-out. Whilst binding activity of TNFRII-Fc and LFA-3-Fc were unchanged by fucose-removal, nonfucosylated Fc fusion proteins exhibited significantly higher Fc receptor gammaIIIa-binding and increased Fc-mediated cytotoxicity on target cells compared to fucosylated counterparts. Notably, in case of TNFRII-Fc, only the nonfucosylated protein exhibited potent Fc dependent cytotoxicity to transmembrane TNF-alpha expressing cells. These results prove that enhancement of Fc dependent cellular cytotoxicity by fucose-removal is effective in not only whole IgG but also Fc fusion proteins, and thus widens the potential of Fc-fusion proteins as therapeutic candidates.
Allergen-specific IgE Detection on Microarrays Using Rolling Circle Amplification: Correlation with in Vitro Assays for Serum IgE (2001)

Allergen-specific IgE antibody in patient serum is used to predict an allergic response in individuals with concordant clinical history. For more than 30 years, in vitro assays for allergen-specific IgE have been used along with or in place of skin-prick allergen testing (SPT) (1). In vitro test methods include various immunoassay formats with solid-phase supports such as paper disks, microtiter plates, nitrocellulose, and microparticles. The field has advanced with immunoassay refinements, including solid phases with higher allergen-binding capacities, monoclonal antibodies for detection, enzyme amplification systems, and fluid-phase allergen/IgE complex formation; these have improved the sensitivity of the in vitro assays and provided better correlation to skin-prick allergen tests (2)(3)(4).

SPT and in vitro allergen-specific IgE assays have advantages and disadvantages. SPT results are available immediately, allowing the allergist to provide treatment while the patient is still in the office. Multiple allergen extracts are tested simultaneously on the same patient. Skin-prick tests have the highest positive predictive value because they are biological, but they also have a high false-positive rate. To be administered in vivo, the allergen extracts must be sterile and of low toxicity to prevent anaphylaxis. SPT is expensive because it requires a skilled practitioner. In vitro serum allergen-specific IgE immunoassays are semiquantitative and allow testing of an allergic response over time (4). They are minimally invasive, with no risk of an adverse reaction in the patient, and are the best option in patients with severe skin conditions such as eczema, urticaria, or dermatographism. In food allergy testing, in vitro tests can reduce by one-half the need for oral allergen challenges (4). In vitro tests can also be used with patients receiving medications, such as antihistamines, that may interfere with SPT responsiveness. However, results of in vitro IgE assays can vary between test formats …
Efficient temporal compression of coherent nanosecond pulses in a compact SBS generator-amplifier setup (1997)

A pulse compressor based on stimulated Brillouin scattering (SBS) in liquids is experimentally and theoretically investigated. It allows for the compression of Fourier-transform limited nanosecond pulses of several hundreds of millijoules of energy with both high conversion efficiency and a high temporal compression factor. The two-cell generator-amplifier arrangement is of a compact design not requiring external attenuation of the generator cell input energy. Pulses from an injection-seeded, frequency-doubled Nd:YAG laser of 300-mJ energy were compressed by a factor variable between 6 and 21 at up to 75% reflectivity. Deviation from unity SBS reflectivity is predominantly determined by optical component losses. The generation of 270-ps pulses with high beam quality was achieved in liquid methanol. These powerful pulses of variable duration are difficult to produce with common laser systems and are highly suited for the generation of high-harmonics in gases.
Combined overexpression of urokinase, urokinase receptor, and plasminogen activator inhibitor-1 is associated with breast cancer progression: An immunohistochemical comparison of normal, benign, and malignant breast tissues (1996)

We showed that overexpression of uPAR, uPA, and its main inhibitor, PAI-1, is a constant feature of invasive ductal breast carcinomas. However, the expression of the above fibrinolytic reactants is not specific for breast cancer since positive staining for these molecules was frequently observed in benign breast lesions as well as in normal breast tissues. The combined increased expression of uPA and its cellular receptor, uPAR on the surface of tumor epithelial cells may account for the activation of the proteolytic system which occurs in breast cancer.
Multi-layered electrostatic film actuator (2002)

A multilayered electrostatic film actuator to realize a high force/weight ratio actuator applicable for large-scale machines is proposed. It has numerous films with plenty of small electrodes stacked and connected. In order to build this structure, an electrostatic actuator named the image charge stepping actuator has been developed. It utilizes image charges induced on a high resistance to produce force. It is fabricated on polymer films and a prototype of the multilayered actuator with four film actuators has been constructed. The fabricated multilayered actuator can produce a force of 0.4 N.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Real-Time Quantitative PCR Detection Method Specific to Widestrike Transgenic Cotton (Event 281-24-236/3006-210-23) (2006)

In compliance with global regulations on transgenic crops, a real-time quantitative PCR method specific to Widestrike transgenic cotton (event 281-24-236/3006-210-23, OECD Unique Identifier DAS-24236-5/DAS-21023-5) was established on the basis of the DNA sequences in the junction between the transgene insert and cotton genome. The optimized method consists of a DNA extraction method for cotton seeds and three PCR systems corresponding to a cotton-specific endogenous reference DNA sequence SAH7 (Sinapis Arabidopsis Homolog 7) and specific detection of event 281-24-236 and event 3006-210-23. The method performance including specificity, sensitivity, accuracy, and precision was determined at a dynamic range of Widestrike DNA levels from 0.04% to 5.0%. The limits of detection (LOD) and quantification (LOQ) were < or =0.04% and < or =0.09%, respectively, at 100 ng of DNA sample per reaction. The quantification results using either the event 281-24-236 or 3006-210-23 system were consistent, and the relative deviation from the expected (true) value was in the range of +/-25%. The robustness of the method was demonstrated by a series of tests with deviations from the optimized assay parameters such as annealing temperature, extension time, PCR instrument, interlaboratory transferability, etc. All the measurements from these tests met the criteria set by EU JRC-CRL (European Commission Joint Research Centre-Community Reference Lab). This real-time quantitative PCR method is accurate and robust, and is recommended as a global benchmark method for the detection and quantification of Widestrike cotton. The method including description, protocol, and performance results is available on the JRC-CRL website (http://gmo-crl.jrc.it/statusofdoss.htm).
Changes in Activated T Cells in the Blood Correlate With Disease Activity in Multiple Sclerosis (2000)

There is a linkage between peripheral T-lymphocyte activation as measured by cell surface markers and disease activity in patients with multiple sclerosis. Arch Neurol. 2000;57:1183-1189
Sirtuin 1 Facilitates Generation of Induced Pluripotent Stem Cells from Mouse Embryonic Fibroblasts through the miR-34a and p53 Pathways (2012)

Forced-expression of transcription factors can reprogram somatic cells into induced pluripotent stem cells (iPSC). Recent studies show that the reprogramming efficiency can be improved by inclusion of small molecules that regulate chromatin modifying enzymes. We report here that sirtuin 1 (SIRT1), a member of the sirtuin family of NAD(+)-dependent protein deacetylases, is involved in iPSC formation. By using an efficient mouse secondary fibroblast reprogramming system with doxycycline (DOX) inducible Yamanaka's transcription factors delivered by piggyBac (PB) transposition (2°F/1B MEF), we show that SIRT1 knockdown decreased while resveratrol (RSV) increased the efficiency of iPSC formation. The treatments were associated with altered acetylated p53 and its downstream Nanog but not p21 expression. The stimulatory effect was also confirmed by SIRT1 over-expression, which stimulated the formation of colonies with induced Nanog and reduced p21 expression. Furthermore, the effects of RSV and SIRT1 knockdown on reprogramming were most pronounced during the initiation phase of reprogramming. MicroRNA-34a is a known regulator of SIRT1. Its inhibitor increased, while its mimics reduced iPSC formation. The stimulatory effect of SIRT1 during reprogramming was also confirmed in the primary MEF. RSV increased while tenovin-6, a small molecule that activates p53 through SIRT1 inhibition, suppressed reprogramming. In conclusion, SIRT1 enhances iPSC generation, in part, through deacetylation of p53, inhibition of p21 and enhancement of Nanog expression.
Vision-guided servoing with feature-based trajectory generation (for robots) (1989)

The authors present a vision module which is able to guide an eye-in-hand robot through general servoing and tracking problems using off-the-shelf image-processing equipment. The vision module uses the location of binary image features from a camera on the robot's end-effector to control the position and one degree of orientation of the robot manipulator. A unique feature-based trajectory generator provides smooth motion between the actual image features and the desired image features even with asynchronous and discontinuous vision updates. By performing the trajectory generation in image feature space, image-processing constraints such as the feature extraction time can be accounted for when determining the appropriate segmentation and acceleration times of the trajectory. Experimental results of a PUMA robot tracking objects with vision feedback are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Modern Views on Desilicification: Biosilica and Abiotic Silica Dissolution in Natural and Artificial Environments (2010)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTModern Views on Desilicification: Biosilica and Abiotic Silica Dissolution in Natural and Artificial EnvironmentsHermann Ehrlich*†, Konstantinos D. Demadis*‡, Oleg S. Pokrovsky§, and Petros G. Koutsoukos∥View Author Information Institute of Bioanalytical Chemistry, Dresden University of Technology, D-01069 Dresden, Germany, Crystal Engineering, Growth and Design Laboratory, Department of Chemistry, University of Crete, Voutes Campus, GR-71003 Heraklion, Crete, Greece, Laboratory of Mechanisms and Transfer in Geology, Observatory Midi-Pyrenees (OMP), UMR 5563, CNRS, 14 Avenue Edouard Belin, 31400 Toulouse, France, and FORTH-ICEHT and Laboratory of Inorganic and Analytical Chemistry, Department of Chemical Engineering, University of Patras, GR-265 04 Patras, Greece* To whom correspondence should be addressed. E-mail addresses: [email protected]; [email protected]†Dresden University of Technology.‡University of Crete.§Observatory Midi-Pyrenees (OMP).∥University of Patras.Cite this: Chem. Rev. 2010, 110, 8, 4656–4689Publication Date (Web):May 4, 2010Publication History Received7 October 2009Published online4 May 2010Published inissue 11 August 2010https://pubs.acs.org/doi/10.1021/cr900334yhttps://doi.org/10.1021/cr900334yreview-articleACS PublicationsCopyright © 2010 American Chemical SocietyRequest reuse permissionsArticle Views4528Altmetric-Citations204LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Bacteria,Dissolution,Nanoparticles,Silica,Silicon Get e-Alerts
Type I transforming growth factor-beta receptors on neutrophils mediate chemotaxis to transforming growth factor-beta (1991)

Abstract Participation of human polymorphonuclear neutrophils in the inflammatory response is mediated, in part, by soluble factors such as chemotactic peptides and cytokines. Although the cytokine, transforming growth factor beta (TGF-beta), has been shown to recruit monocytes and promote the inflammatory process, its effects on neutrophils are unknown. In this investigation, [125I]TGF-beta 1 affinity binding studies were employed to show that neutrophils express TGF-beta receptors (350 +/- 20 receptors/cell), which exhibit high affinity for the ligand (dissociation constant, 50 pM). Affinity cross-linking studies identified the receptors to be primarily of the type I class. In contrast to the receptors on monocytes, neutrophil TGF-beta receptors were not down-regulated by exposure to specific inflammatory mediators. Additional studies examined whether exposure of neutrophils to TGF-beta could enhance specific functions, as occurs with monocytes. TGF-beta was shown to cause directed migration of neutrophils at femtomolar concentrations, thus it is the most potent neutrophil chemotactic factor yet identified. Neutrophil production of reactive oxygen intermediates was not stimulated by TGF-beta, nor did TGF-beta enhance or depress subsequent PMA- or FMLP-stimulated superoxide production. However, the stable expression of neutrophil TGF-beta receptors, and the capacity of this cytokine to stimulate neutrophil chemotaxis, suggest that the pro-inflammatory effects of TGF-beta are mediated by neutrophils in addition to monocytes.
SERIALS FUNDS: A SURPLUS IS PREDICTED (1989)

Managing the serials budget presents an ongoing challenge to librarians. Today, no other budget item is scrutinized as carefully as serials expenditures. Information carries an ever-escalating price tag; the process of turning information into a published format is an expensive one. There are people costs, editing costs, printing costs, paper costs, binding costs, packaging costs and fulfillment costs. While our society recognizes access to information as a fundamental right of each individual, librarians today find themselves faced with the loaded problem of deciding what serial tides to cancel or what important new ones to simply not purchase.
Chitosan Oligosaccharide-Stabilized Ferrimagnetic Iron Oxide Nanocubes for Magnetically Modulated Cancer Hyperthermia (2012)

Magnetic nanoparticles have gained significant attention as a therapeutic agent for cancer treatment. Herein, we developed chitosan oligosaccharide-stabilized ferrimagnetic iron oxide nanocubes (Chito-FIONs) as an effective heat nanomediator for cancer hyperthermia. Dynamic light scattering and transmission electron microscopic analyses revealed that Chito-FIONs were composed of multiple 30-nm-sized FIONs encapsulated by a chitosan polymer shell. Multiple FIONs in an interior increased the total magnetic moments, which leads to localized accumulation under an applied magnetic field. Chito-FIONs also exhibited superior magnetic heating ability with a high specific loss power value (2614 W/g) compared with commercial superparamagnetic Feridex nanoparticles (83 W/g). The magnetically guided Chito-FIONs successfully eradicated target cancer cells through caspase-mediated apoptosis. Furthermore, Chito-FIONs showed excellent antitumor efficacy on an animal tumor model without any severe toxicity.
A novel gene mutation that confers abnormal patterns of β‐carotene accumulation in cauliflower (Brassica oleracea var. botrytis) (2001)

The Or gene of cauliflower (Brassica oleracea var. botrytis) causes many tissues of the plant to accumulate carotenoids and turn orange, which is suggestive of a perturbation of the normal regulation of carotenogenesis. A series of experiments to explore the cellular basis of the carotenoid accumulation induced by the Or gene was completed. The Or gene causes obvious carotenoid accumulation in weakly or unpigmented tissues such as the curd, pith, leaf bases and shoot meristems, and cryptically in some cells of other organs, including the roots and developing fruits. The dominant carotenoid accumulated is beta-carotene, which can reach levels that are several hundred-fold higher than those in comparable wild-type tissues. The beta-carotene accumulates in plastids mainly as a component of massive, highly ordered sheets. The Or gene does not affect carotenoid composition of leaves, nor does it alter color and chromoplast appearance in flower petals. Interestingly, mRNA from carotenogenic and other isoprenoid biosynthetic genes upstream of the carotenoid pathway was detected both in orange tissues of the mutant, and in comparable unpigmented wild-type tissues. Thus the unpigmented wild-type tissues are likely to be competent to synthesize carotenoids, but this process is suppressed by an unidentified mechanism. Our results suggest that the Or gene may induce carotenoid accumulation by initiating the synthesis of a carotenoid deposition sink in the form of the large carotenoid-sequestering sheets.
ChemInform Abstract: FENTANYL ANALOGS. 3. 1,2,3,4‐TETRAHYDRO‐2‐NAPHTHYL SUBSTITUTED 4‐ANILIDOPIPERIDINES (1985)

Abstract Auf verschiedenen Wegen werden die Fentanyl‐Analogen (VII) einerseits und (XIII) andererseits dargestellt [bei der Reduktion des Anils (IX) fällt (X) als Diastereomerengemisch an, das sich chromatographisch an Silicagel auftrennen läßt: 66% (Xa) und 34% (Xb) in bezug auf das gereinigte Produkt (X)].
Distinct Licensing of IL-18 and IL-1β Secretion in Response to NLRP3 Inflammasome Activation (2012)

Inflammasome activation permits processing of interleukins (IL)-1β and 18 and elicits cell death (pyroptosis). Whether these responses are independently licensed or are "hard-wired" consequences of caspase-1 (casp1) activity has not been clear. Here, we show that that each of these responses is independently regulated following activation of NLRP3 inflammasomes by a "non-canonical" stimulus, the secreted Listeria monocytogenes (Lm) p60 protein. Primed murine dendritic cells (DCs) responded to p60 stimulation with reactive oxygen species (ROS) production and secretion of IL-1β and IL-18 but not pyroptosis. Inhibitors of ROS production inhibited secretion of IL-1β, but did not impair IL-18 secretion. Furthermore, DCs from caspase-11 (casp11)-deficient 129S6 mice failed to secrete IL-1β in response to p60 but were fully responsive for IL-18 secretion. These findings reveal that there are distinct licensing requirements for processing of IL-18 versus IL-1β by NLRP3 inflammasomes.