gebhart/patrepeval huggingface dataset:
Prevalence of Campylobacter , Arcobacter , Helicobacter , and Sutterella spp. in Human Fecal Samples as Estimated by a Reevaluation of Isolation Methods for Campylobacters (2000)

The aims of this study were to investigate the prevalence of campylobacteria including Campylobacter jejuni subsp. jejuni (C. jejuni) and Campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of Campylobacter spp. Two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mCCDA) and cefoperazone-amphotericin-teicoplanin (CAT) agar, were compared with Skirrow's blood-based medium and with a filter method (filter) applied to a yeast-enriched blood agar. A total of 1,376 specimens were tested on all four media, and the percentages of thermophilic Campylobacter-positive specimens isolated on Skirrow's medium, filters, CAT agar, and mCCDA were 82, 83, 85, and 95%, respectively. When additional samples were processed with the three selective media, mCCDA recovered significantly more thermophilic Campylobacter spp. than Skirrow's medium (P = 0.0034). No significant difference between Skirrow's medium and CAT agar was observed in this study. Another six taxa were identified, namely, Campylobacter concisus, Campylobacter curvus-like bacteria, Arcobacter butzleri, Arcobacter cryaerophilus, Helicobacter cinaedi, and Sutterella wadsworthensis. Most of these strains were isolated after 5 to 6 days of incubation by use of the filter technique. This paper provides evidence for the existence of S. wadsworthensis in human feces from clinical cases of gastrointestinal disorders and in feces from a healthy individual. Furthermore, C. concisus was isolated from a large number of diarrheal cases, particularly those at the extremes of age, but was additionally isolated from the feces of healthy people. Further investigations to establish the role of C. concisus and S. wadsworthensis in enteric disease is needed. We conclude that a range of campylobacteria may cause infections in Denmark.
Prostatic steroid 5 alpha-reductase, an androgen metabolic gene. (2000)

Prostate cancer risk is highest in African Americans, lowest in Asians, and intermediate in Caucasians and Latinos. The data clearly suggest that environmental and genetic factors are involved. Investigation of the genetic factors suggests that allelic variation in the SRD5A2 gene is partially responsible for the striking racial and ethnic variations in risk.
Waveform-aware ambient RF energy harvesting (2014)

In this paper we suggest a new class of RF energy harvesters, which we call “waveform aware harvesters”. In contrast to traditional rectenna designs, which are usually designed for high efficiency with continuous wave (CW) signals, waveform aware harvesters are RF to DC converters which are optimized for their performance with non-CW signals. We suggest that waveform aware harvesters may have significant advantages in ambient energy harvesting, where the available RF energy is in the form of communication waveforms of a variety of types. We present an initial proof-of-concept demonstration of a waveform aware harvester optimized for harvesting energy from 2.4 GHz Wi-Fi (802.11b/g) signals with a realistic traffic model. Under realistic traffic conditions, 802.11b/g client transmissions are bursty, with a high peak-to-average ratio and a low duty cycle. We demonstrate optimized recovery of harvested energy from single 802.11b/g transmission bursts on the order of 1 ms in duration. We present an expression for maximizing usable energy stored in an energy reservoir given a signal model and parameters of the energy-harvester circuit. In contrast to other work where assumptions of CW sources lead to the desirability of a large storage capacitor, our approach considers the existing communication signal model and optimizes capacitor size to maximize the stored usable energy for a short transmission burst.
Phenotypic and Interaction Profiling of the Human Phosphatases Identifies Diverse Mitotic Regulators (2016)

Reversible phosphorylation is a fundamental regulatory mechanism, intricately coordinated by kinases and phosphatases, two classes of enzymes widely disrupted in human disease. To better understand the functions of the relatively understudied phosphatases, we have used complementary affinity purification and proximity-based interaction proteomics approaches to generate a physical interactome for 140 human proteins harboring phosphatase catalytic domains. We identified 1,335 high-confidence interactions (1,104 previously unreported), implicating these phosphatases in the regulation of a variety of cellular processes. Systematic phenotypic profiling of phosphatase catalytic and regulatory subunits revealed that phosphatases from every evolutionary family impinge on mitosis. Using clues from the interactome, we have uncovered unsuspected roles for DUSP19 in mitotic exit, CDC14A in regulating microtubule integrity, PTPRF in mitotic retraction fiber integrity, and DUSP23 in centriole duplication. The functional phosphatase interactome further provides a rich resource for ascribing functions for this important class of enzymes.
On the Convergent Evolution of Animal Toxins (1997)

BgK is a K+ channel-blocking toxin from the sea anemone Bunodosoma granulifera. It is a 37-residue protein that adopts a novel fold, as determined by NMR and modeling. An alanine-scanning-based analysis revealed the functional importance of five residues, which include a critical lysine and an aromatic residue separated by 6.6 +/- 1.0 A. The same diad is found in the three known homologous toxins from sea anemones. More strikingly, a similar functional diad is present in all K+ channel-blocking toxins from scorpions, although these toxins adopt a distinct scaffold. Moreover, the functional diads of potassium channel-blocking toxins from sea anemone and scorpions superimpose in the three-dimensional structures. Therefore, toxins that have unrelated structures but similar functions possess conserved key functional residues, organized in an identical topology, suggesting a convergent functional evolution for these small proteins.
Hexatic and crystal phase transitions in thin free-standing liquid-crystal films (1991)

Thermal and structural studies have been performed on thin films of n-heptyl-4'-n-pentyloxylbiphenyl-4-carboxylate (75OBC). Similar to 65OBC, a surface hexatic phase is observed for films greater than four molecular layers in thickness. Four- and three-layer films display a single, two-dimensional liquid-hexatic transition. The four-layer heat-capacity anomaly is described well by a divergent power-law expression. The three-layer heat-capacity anomaly is qualitatively the same, although its size is greatly reduced. Also, a surface crystal-E phase is observed immediately below the liquid-hexatic transition(s).
Selective reduction of carboxylic acids into alcohols using sodium borohydride and iodine (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelective reduction of carboxylic acids into alcohols using sodium borohydride and iodineJ. V. Bhaskar Kanth and Mariappan PeriasamyCite this: J. Org. Chem. 1991, 56, 20, 5964–5965Publication Date (Print):September 1, 1991Publication History Published online1 May 2002Published inissue 1 September 1991https://pubs.acs.org/doi/10.1021/jo00020a052https://doi.org/10.1021/jo00020a052research-articleACS PublicationsRequest reuse permissionsArticle Views13752Altmetric-Citations129LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Thermomechanical Behavior of a Polyurethane Shape Memory Polymer Foam (2006)

Shape memory polymers (SMPs) have attracted great interest in recent years. The SMP foams are outstanding, owing to their high shape recovery ratio in compression. They can be used for, for instance, micro foldable vehicles, shape determination, and microtags. This article presents a study on the thermomechanical behavior of a polyurethane SMP foam, associated with these three applications. This includes four types of tests namely, compression test, free recovery test, constrained cooling test, and gripping test.
LethalEscherichia coli andSalmonella typhimurium endotoxemia is mediated through different pathways (2001)

Despite the differences in the molecular structure between lipopolysaccharides (LPS) isolated from Escherichia coli, Klebsiella pneumoniae or Salmonella typhimurium, the potential differences in their biological effects in vivo have not been investigated. In the present study, TNF and LT double knock-out (TNF-/-LT-/-) mice were almost as susceptible as TNF+/+LT+/+ controls to S. typhimurium LPS, but they were significantly more resistant to lethal endotoxemia induced by E. coli or K. pneumoniae LPS. The effect was not due to endotoxin-associated proteins. In the knock-out mice, this difference in lethality was accompanied by decreased interleukin-1 (IL-1) and interferon-gamma (IFN-gamma) production after challenge with E. coli LPS, whereas after S. typhimurium LPS more IL-1 and IFN-gamma were produced. In contrast, more IL-10 was produced after challenge of mice with E. coli LPS than with S. typhimurium LPS. The hypothesis that a combination of pro-inflammatory cytokines is responsible for the mortality after S. typhimurium LPS was suggested by experiments in mice deficient in IL-1beta-converting enzyme (ICE-/- mice). ICE-/-mice, lacking mature IL-1beta and IL-18, but also defective in IFN-gamma and TNF production, were completely protected against both E. coli and S. typhimurium LPS. Experiments in Toll-like receptor (TLR)-4 defective mice suggested that the difference is not due to differential activation of TLR4. In conclusion, TNF and LT play a central role in the lethality due to E. coli LPS, whereas the lethal effects of S. typhimurium LPS are mediated through mechanisms also involving other cytokines such as IFN-gamma, IL-1 and IL-18.
National Sentiment and Migration in Soviet Central Asia (1987)

The Program of the CPSU adopted at the 27th Party Congress (1986) included a guarded reference to the nationality question. It observed that the “nationality question, as it has been inheritedfrom the past , has been successfully solved.” This reference to nationality problems as a remnant of the fading capitalist past was not designed to suggest that problems of a national character have ceased to exist in the Soviet Union today. Gorbachev made this point to the Congress delegates noting: “Our achievements should not create the impression that there are no complications in nationality processes. The contradictions characteristic of all development are unavoidable in this sphere as well.” The violent outbursts of a national character in Kazakhstan in December 1986 provide fresh evidence that ethnic tensions lurk just beneath the surface. These tensions have been held in check largely by the threat of reprisals from central authorities who have regarded meaningful expressions of nationalism as treasonous.
Comparison of solutions for preservation of the rabbit liver as tested by isolated perfusion (1995)

Abstract The University of Wisconsin (UW) solution consists of a relatively complex mixture of agents. In this study we compared simpler preservation solutions, namely, histidine-tryptophan-ketoglutarate (HTK) and phosphate-buffered sucrose (PBS) with different compositions of UW solution in the isolated perfused rabbit liver model. Livers were stored cold for 24 and 48 h. After 24 h of preservation, the amount of bile produced in UW-preserved livers was significantly greater (P < 0.05) than that in HTK-preserved livers. Also, there was less LDH released into the perfusate in UW-preserved livers. There was more edema and lower K +/Na + rations in HTK-preserved livers than in UW-preserved livers (all data P < 0.05). After 48 h of preservation, the differences between livers preserved in UW or HTK solution were less noticeable than at 24 h and bile production was similar. LDH and AST release were greater in HTK-preserved livers than in UW livers, but these differences were not statistically significant. Preservation in PBS for 48 h was worse than in either UW or HTK solution. Substitution of polyethylene glycol (PEG) for hydroxyethyl starch (HES) in 48-h UW-preserved livers was not effective. We conclude that solutions simpler in composition than UW solution may be effective in kidney transplantation but do not appear suitable for successful liver preservation.
CXCL8/IL‐8 and CXCL12/SDF‐1α co‐operatively promote invasiveness and angiogenesis in pancreatic cancer (2008)

CXC-chemokines are involved in the chemotaxis of neutrophils, lymphocytes and monocytes. However, role of these chemokines in tumorigenesis, especially with regard to interaction between tumor and its microenvironment, has not been clearly elucidated. The purpose of this study was to analyze the co-operative role of CXCL8 and CXCL12 in the tumor-stromal interaction in pancreatic cancer (PaCa). Using enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR), we initially confirmed the expression of ligands and receptors, respectively, of CXC-chemokines in PaCa and stromal cells. We examined the co-operative role of CXCL8 and CXCL12 in proliferation/invasion of PaCa and human umbilical vein endothelial cells (HUVECs), and in HUVEC tube-formations through tumor-stromal interaction by MTS, Matrigel invasion, and angiogenesis assays, respectively. We detected expression of CXCR4, but not CXCR2, in all PaCa cells and fibroblasts. PaCa cells secreted CXCL8, and fibroblast cells secreted CXCL12. CXCL8 production in PaCa was significantly enhanced by CXCL12, and CXCL12 production in fibroblasts was significantly enhanced by co-culturing with PaCa. CXCL8 enhanced proliferation/invasion of HUVECs but did not promote proliferation/invasion of PaCa. Both recombinant and PaCa-derived CXCL8 enhanced tube formation of HUVECs that were co-cultured with fibroblast cells. CXCL12 enhanced the proliferation/invasion of HUVECs and the invasion of PaCa cells but had no effect on tube formation of HUVEC. We showed that PaCa-derived CXCL8 and fibroblast-derived CXCL12 cooperatively induced angiogenesis in vitro by promoting HUVEC proliferation, invasion, and tube formation. Thus, corresponding receptors CXCR2 and CXCR4 are potential antiangiogenic and antimetastatic therapeutic targets in PaCa.
<title>Information-theoretic approach to management in decentralized data fusion</title> (1992)

In this paper we present an information based framework for addressing multi-sensor data fusion and its management. The basis for this approach is the notion of Bayesian Information Update from which we present a probabilistic model for data fusion and its management. We proceed to outline how architectures and algorithms can be derived from the information update. This leads to a framework for sensor management based on using information as the expected utility of taking actions. We show how Fisher information and more generally Entropy can be used to quantify information. We conclude by briefly outlining a vehicle application making use of data fusion algorithms and sensor management techniques that we present.
Denmark health system review. (2012)

Denmark has a tradition of a decentralized health system. However, during recent years, reforms and policy initiatives have gradually centralized the health system in different ways. The structural reform of 2007 merged the old counties into fewer bigger regions, and the old municipalities likewise. The hospital structure is undergoing similar reforms, with fewer, bigger and more specialized hospitals. Furthermore, a more centralized approach to planning and regulation has been taking place over recent years. This is evident in the new national planning of medical specialties as well as the establishment of a nationwide accreditation system, the Danish Healthcare Quality Programme, which sets national standards for health system providers in Denmark. Efforts have also been made to ensure coherent patient pathways - at the moment for cancer and heart disease - that are similar nationwide. These efforts also aim at improving intersectoral cooperation. Financially, recent years have seen the introduction of a higher degree of activity-based financing in the public health sector, combined with the traditional global budgeting.A number of challenges remain in the Danish health care system. The consequences of the recent reforms and centralization initiatives are yet to be fully evaluated. Before this happens, a full overview of what future reforms should target is not possible. Denmark continues to lag behind the other Nordic countries in regards to some health indicators, such as life expectancy. A number of risk factors may be the cause of this: alcohol intake and obesity continue to be problems, whereas smoking habits are improving. The level of socioeconomic inequalities in health also continues to be a challenge. The organization of the Danish health care system will have to take a number of challenges into account in the future. These include changes in disease patterns, with an ageing population with chronic and long-term diseases; ensuring sufficient staffing; and deciding how to improve public health initiatives that target prevention of diseases and favour health improvements.
Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set (2002)

The design and evaluation of a set of universal primers and probe for the amplification of 16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is reported. Broad specificity of the universal detection system was confirmed by testing DNA isolated from 34 bacterial species encompassing most of the groups of bacteria outlined in Bergey's Manual of Determinative Bacteriology. However, the nature of the chromosomal DNA used as a standard was critical. A DNA standard representing those bacteria most likely to predominate in a given habitat was important for a more accurate determination of total bacterial load due to variations in 16S rDNA copy number and the effect of generation time of the bacteria on this number, since rapid growth could result in multiple replication forks and hence, in effect, more than one copy of portions of the chromosome. The validity of applying these caveats to estimating bacterial load was confirmed by enumerating the number of bacteria in an artificial sample mixed in vitro and in clinical carious dentine samples. Taking these parameters into account, the number of anaerobic bacteria estimated by the universal probe and primers set in carious dentine was 40-fold greater than the total bacterial load detected by culture methods, demonstrating the utility of real-time PCR in the analysis of this environment.
Transverse relaxation of solvent protons induced by magnetized spheres: Application to ferritin, erythrocytes, and magnetite (1987)

Since 1/T2 of protons of tissue water is generally much greater than 1/T1 at typical imaging fields, small single-ion contrast agents--such as Gd(DTPA), which make comparable incremental contributions and therefore smaller fractional contributions to 1/T2 compared to 1/T1--are not as desirable for contrast-enhancement as agents that could enhance 1/T2 preferentially. In principle, such specialized agents will only be effective at higher fields because the field dependence (dispersion) of 1/T1 is such that it approaches zero at high fields whereas 1/T2 approaches a constant value. The residual 1/T2 is called the "secular" contribution and arises from fluctuations in time--as sensed by the protons of diffusing solvent or tissue water molecules--of the component of the magnetic field parallel to the static applied field. For solutions or suspensions of sufficiently large paramagnetic or ferromagnetic particles (greater than or equal to 250 A diameter), the paramagnetic contributions to the relaxation rates satisfy 1/T2 much greater than 1/T1 at typical imaging fields. We examine the theory of secular relaxation in some detail, particularly as it applies to systems relevant to magnetic resonance imaging, and then analyze the data for solutions, suspensions, or tissue containing ferritin, erythrocytes, agar-bound magnetite particles, and liver with low-density composite polymer-coated magnetite. In most cases we can explain the relaxation data, often quantitatively, in terms of the theory of relaxation of protons (water molecules) diffusing in the outer sphere environments of magnetized particles. The dipolar field produced by these particles has a strong spatial dependence, and its apparent fluctuations in time as seen by the diffusing protons produce spin transitions that contribute to both 1/T1 and /T2 comparably at low fields; for the larger particles, because of dispersion, the secular term dominates at fields of interest. On the basis of the agreement of theory with data for solutions of small paramagnetic complexes, large magnetite particles, and liver containing low-density polymer-coated magnetite agglomerates, it is argued that the theory is sufficiently reliable so that, e.g., for ferritin--for which 1/T2 is unexpectedly large--the source of its large relaxivity must reside in nonideal chemistry of the ferritin core. For blood, it appears that diffusion through intracellular gradients determines 1/T2.