gebhart/patrepeval huggingface dataset:
Distinct C/EBP functions are required for eosinophil lineage commitment and maturation (1998)

Hematopoietic differentiation involves the commitment of multipotent progenitors to a given lineage, followed by the maturation of the committed cells. To study the transcriptional events controlling these processes, we have investigated the role of C/EBP proteins in lineage choice of multipotent hematopoietic progenitors (MEPs) transformed by the E26 virus. We found that forced expression of either the alpha or beta isoforms of C/EBP in MEPs induced eosinophil differentiation and that in addition, C/EBPbeta could induce myeloid differentiation. Conversely, dominant-negative versions of C/EBPbeta inhibited myeloid differentiation. C/EBP-induced eosinophil differentiation could be separated into two distinct events, lineage commitment and maturation. Thus, eosinophils induced by transactivation-deficient C/EBPbeta alleles were found to be blocked in their maturation, whereas those expressing wild-type C/EBP proteins were not. Likewise, a 1-day activation of a conditional C/EBPbeta allele in multipotent progenitors led to the formation of immature eosinophils, whereas sustained activation produced mature eosinophils. These results show that C/EBP can induce both myeloid and eosinophil lineage commitment and that transactivation independent and dependent C/EBP functions are required during eosinophil lineage commitment and maturation, respectively.
Preparation and Characterization of Functional Poly(ethylene glycol) Surfaces for the Use of Antibody Microarrays (2007)

Protein microarrays serve as measurement platforms for multianalytical applications. Small molecules, DNA, proteins, and cells are determined quantitatively. Amino-PEG surfaces can be a smart functional platform for protein microarrays with high signal-to-noise ratios. An effective step-by-step chemistry is developed for uniform presentation of terminal functional groups at each monolayer. Poly(ethelene glycol diamine) 2000 (DAPEG, 2000 g/mol) films were prepared onto silanized glass slides presenting epoxy groups. The uniformity of the grafted DAPEG monolayer is characterized by a chemiluminescence reaction using a chemiluminescence microarray reader with automated reagent supply and a horseradish peroxidase (HRP)/luminol reporter system. An intensity line plot on the horizontal axis was generated. The chemiluminescence intensities vary in a range of 2.6%. Antibodies against HRP as model system were immobilized on N-hydroxysuccinimide activated DAPEG layers by means of a microcontact roboter system. Chemiluminescence signals of bound HRP are detected at each spot with a standard deviation of 2.9%. The maximum antibody concentration that can be immobilized at the surface is determined with 1 mg/mL. Additives for an optimal spotting buffer are also studied. The use of the block-copolymer Pluronic F127 as antibody stabilizer is as well investigated as trehalose for the prevention of spot evaporation. The lowest detectable HRP concentration is 0.08 ng/mL determined on anti-HRP antibody microarrays. This study demonstrates how surfaces and analytical parameters for protein microarray applications can be characterized with a chemiluminescence readout system using a HRP reporter system.
Structure of an Agonist-Bound Human A 2A Adenosine Receptor (2011)

Activation of G protein-coupled receptors upon agonist binding is a critical step in the signaling cascade for this family of cell surface proteins. We report the crystal structure of the A(2A) adenosine receptor (A(2A)AR) bound to an agonist UK-432097 at 2.7 angstrom resolution. Relative to inactive, antagonist-bound A(2A)AR, the agonist-bound structure displays an outward tilt and rotation of the cytoplasmic half of helix VI, a movement of helix V, and an axial shift of helix III, resembling the changes associated with the active-state opsin structure. Additionally, a seesaw movement of helix VII and a shift of extracellular loop 3 are likely specific to A(2A)AR and its ligand. The results define the molecule UK-432097 as a "conformationally selective agonist" capable of receptor stabilization in a specific active-state configuration.
The electron effective mass in heavily doped GaAs (1979)

A resolution to the order k6 of the three-level equation of Kane allows the authors to obtain a new expression for the conduction band energy and so new analytical expressions for the electron effective masses in the range of carrier concentration 1016-1019 cm-3. The calculated values of the conduction effective mass as a function of carrier density agree well, for the first time, with experimental values, which were obtained from Shubnikov-de Haas experiments by using a new expression for the oscillation amplitude. In this expression the authors take into account the effect of medium-range potential fluctuations induced by crystal inhomogeneities.
In vivo murine tumor gene delivery and expression by systemic recombinant vaccinia virus encoding interleukin-1beta. (2006)

These data show that recombinant vaccinia virus encoding hIL-1beta given intravenously preferentially localizes and amplifies in tumor tissue, is rapidly cleared from liver and spleen, produces measurable hIL-1beta in tumor but not normal tissues, and inhibits growth of established solid tumors in mice. Recombinant vaccinia virus encoding therapeutic genes may be a practical, efficient vehicle for direct in vivo gene transfer and expression in the treatment of cancer.
Evaluation of iliopsoas compartment disorders by computed tomography. (1995)

It is difficult to differentiate iliopsoas abscesses, hematomas and neoplasms without knowing the clinical history. However, when coupled with percutaneous aspiration biopsy, CT has proved to be an effective, rapid means to diagnose and guide the therapy of psoas lesions.
Modeling deformable human hands from medical images (2004)

This paper presents a new method for constructing an example-based deformable human hand model from medical images. Realistic animation of human hands requires good estimates of the joint structure and properly weighted skeleton-driven surface deformation. For this purpose, we propose a method based on medical images of hands in several poses. Our method consists of the following 3 steps: First, using the measured bone shapes, we estimate the link structure (joint rotation centers) and the joint angles of each scan. Second, we construct a mutually consistent polygonal mesh of all the scans. For this purpose, a polygonal mesh of one pose, the base mesh, is deformed using skeletal subspace deformation, and then fitted interactively to the measured meshes from the other scans. Finally, the hand is deformed using a weighted pose space deformation. We demonstrate results of deformable hand models consisting of 100,000 triangle meshes derived from CT scans.
UNNATURAL ENANTIOMERS OF 5-AZACYTIDINE ANALOGUES: SYNTHESES AND ENZYMATIC PROPERTIES (2001)

2'-Deoxy-beta-L-5-azacytidine(L-Decitabine), beta-L-5-azacytidine, and derivatives were stereospecifically prepared starting from L-ribose or L-xylose. D- and L-enantiomers of 2'-deoxy-beta-5-azacytidine were weak substrates of human recombinant deoxycytidine kinase (dCK), whereas both enantiomers of beta-5-azacytidine or the L-xylo-analogues were not substrates of the enzyme. None of the reported derivatives of beta-L-5-azacytidine was a substrate of human recombinant cytidine deaminase (CDA).
Effect of epiregulin on pancreatic beta cell growth and insulin secretion (2005)

The aim of this study was to determine whether epiregulin, a novel member of EGF-related growth factor family, was able to affect proliferation and secretory function of rat insulinoma INS-1E and RINm5F cell lines. A 24 h treatment with epiregulin resulted in a stimulation of INS-1E and RINm5F cells proliferation; this effect was completely blocked in the presence of an anti-epiregulin antibody which did not affect basal DNA synthesis in the absence of added ligand. In acute experiments, epiregulin was able to potentiate insulin release in the presence of glucose or arginine, in the two cell lines. Finally, in the two cell lines expressing ErbB receptors, we demonstrated that only EGFR/ErbB1 was activated by epiregulin. Thus, epiregulin appears as a new growth and insulinotropic factor in pancreatic beta cell lines.
Mouth gesture and voice command based robot command interface (2009)

In this paper we present a voice command and mouth gesture based robot command interface which is capable of controlling three degrees of freedom. The gesture set was designed in order to avoid head rotation and translation, and thus relying solely in mouth movements. Mouth segmentation is performed by using the normalized a* component, as in J. Gomez, et al., (October 2008). The gesture detection process is carried out by a Gaussian mixture model (GMM) based classifier. After that, a state machine stabilizes the system response by restricting the number of possible movements depending on the initial state. Voice commands are modeled using a hidden Markov model (HMM) isolated word recognition scheme. The interface was designed taking into account the specific pose restrictions found in the DaVinci assisted surgery command console.
Expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human chondrosarcomasNote (2001)

The aim of the present study was to characterise the ability of malignant chondrosarcomas to invade normal bone by analysing their production of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). For this purpose 12 chondrosarcomas were investigated for the expression of mRNAs for several MMPs and all 4 TIMPs by Northern hybridisation, and for immunohistochemical localisation of the proteins. A characteristic finding of these analyses was increased expression of MMP-13, MMP-14 and TIMP-2 mRNAs in chondrosarcomas when compared with nonmalignant control samples. Individual chondrosarcomas also exhibited elevated levels of MMP-1, MMP-7 and MMP-9 mRNAs. The results of Northern hybridisations were supported by immunohistochemical stainings of the corresponding tumour areas for MMP-2, MMP-14 and TIMP-2, further suggesting that these may have prognostic value for determining whether individual chondrosarcomas are locally aggressive or have a probability of recurrence. Another finding of the present study was a marked heterogeneity in histologic appearance and gene expression of the chondrosarcomas, emphasising the importance of analysing several areas of these tumours to get representative results. These findings suggest that analysis of MMPs could be a useful diagnostic indicator in patients with cartilaginous tumours and could help in differentiating between a low-grade malignant chondrosarcoma and a benign growing enchondroma.
Peptide design by artificial neural networks and computer-based evolutionary search (1998)

A technique for systematic peptide variation by a combination of rational and evolutionary approaches is presented. The design scheme consists of five consecutive steps: (i) identification of a "seed peptide" with a desired activity, (ii) generation of variants selected from a physicochemical space around the seed peptide, (iii) synthesis and testing of this biased library, (iv) modeling of a quantitative sequence-activity relationship by an artificial neural network, and (v) de novo design by a computer-based evolutionary search in sequence space using the trained neural network as the fitness function. This strategy was successfully applied to the identification of novel peptides that fully prevent the positive chronotropic effect of anti-beta1-adrenoreceptor autoantibodies from the serum of patients with dilated cardiomyopathy. The seed peptide, comprising 10 residues, was derived by epitope mapping from an extracellular loop of human beta1-adrenoreceptor. A set of 90 peptides was synthesized and tested to provide training data for neural network development. De novo design revealed peptides with desired activities that do not match the seed peptide sequence. These results demonstrate that computer-based evolutionary searches can generate novel peptides with substantial biological activity.
A mm-Wave quadrature VCO based on magnetically coupled resonators (2011)

Search of a compact quadrature generator at around 60GHz with low phase noise at moderate power is the topic of this work. Discarding a double-frequency VCO followed by dividers-by-two given the high frequency range of operation, the most suitable topology borrowed by RF solutions is represented by cross-coupled LC voltage-controlled oscillators. However, the oscillation frequency dependence on the biasing current makes it susceptible to phase noise, close-in in particular. At mm-Waves, this is exacerbated by core devices of small dimensions to such an extent that 1/f noise remains dominant up to more than ~10MHz, making it unsuitable for stringent applications. On the contrary a ring of two VCOs magnetically coupled to each other has an oscillation frequency dependence on inter-stage passive components only, low 1/f noise together with good quadrature accuracy. The quadrature oscillator has been realized in a 65nm CMOS technology and prototypes show the following performances: 56-to-60.3GHz tunable oscillation frequency, phase noise better than -95dBc/Hz at 1MHz offset in the tuning range, 1.5° maximum phase error while consuming 22mA from a 1V supply.
Kidney Injury Molecule-1 (KIM-1), a Putative Epithelial Cell Adhesion Molecule Containing a Novel Immunoglobulin Domain, Is Up-regulated in Renal Cells after Injury (1998)

We report the identification of rat and human cDNAs for a type 1 membrane protein that contains a novel six-cysteine immunoglobulin-like domain and a mucin domain; it is named kidney injury molecule-1 (KIM-1). Structurally, KIM-1 is a member of the immunoglobulin gene superfamily most reminiscent of mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Human KIM-1 exhibits homology to a monkey gene, hepatitis A virus cell receptor 1 (HAVcr-1), which was identified recently as a receptor for the hepatitis A virus. KIM-1 mRNA and protein are expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. In situ hybridization and immunohistochemistry revealed that KIM-1 is expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. Structure and expression data suggest that KIM-1 is an epithelial cell adhesion molecule up-regulated in the cells, which are dedifferentiated and undergoing replication. KIM-1 may play an important role in the restoration of the morphological integrity and function to postischemic kidney.
First genomic sequence of a human lg variable lambda gene belonging to subgroup III (1990)

Journal Article First genomic sequence of a human lg variable lambda gene belonging to subgroup III Get access J.-P. Frippiat, J.-P. Frippiat Search for other works by this author on: Oxford Academic PubMed Google Scholar P. Chuchana, P. Chuchana Search for other works by this author on: Oxford Academic PubMed Google Scholar F. Bernard, F. Bernard Search for other works by this author on: Oxford Academic PubMed Google Scholar L. Buluwela, L. Buluwela 1Department of Biochemistry, Charing Cross and Westminster Medical School, University of LondonCharing Cross Hospital, Fulham Palace Road, London W6 8RF, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar G. Lefranc, G. Lefranc Search for other works by this author on: Oxford Academic PubMed Google Scholar M.-P. Lefranc M.-P. Lefranc * * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 23, 1 December 1990, Page 7134, https://doi.org/10.1093/nar/18.23.7134 Published: 01 January 1990 Article history Published: 01 January 1990 Received: 31 October 1990
Proliferation and differentiation of mesenchymal stem cells using self-assembled peptide amphiphile nanofibers (2006)

The objective of this study is to enhance the proliferation and differentiation of mesenchymal stem cells (MSC) in nanodimensional scaffolds. The proliferation and differentiation of MSC was investigated in a three-dimensional network of nanofibers formed by self-assembly of peptide amphiphile (PA) molecules. PA was synthesized by standard solid phase chemistry that ends with the alkylation of the NH(2) terminus of the peptide. The sequence of arginine-glycine-aspartic acid was included in the peptide design as well. A three-dimensional network of nanofibers was formed by mixing MSC suspensions in media with dilute aqueous solution of PA. The attachment, proliferation and osteogenic differentiation of MSC were influenced by the self-assembled PA nanofibers as the cell scaffold and the values were significantly high compared with those in the static culture. The alkaline phosphatase activity and osteocalcin content of MSC cultured in the PA nanofibers significantly increased compared with the static culture method. It may be concluded that PA nanofibers enable MSC to positively improve the proliferation and differentiation extent.
Diffusion of Hydrogen in Natural and Synthetic Fused Quartz (1963)

A mass spectrometer study was made to compare the transport characteristics of hydrogen and deuterium through hollow, thin walled cylinders of three types of fused quartz. The experiments in the temperature range (300°—1000°C) resulted in the following observations: (1) The permeability of hydrogen (and deuterium) in fused quartz is virtually independent of purity level or hydroxyl content. (2) The corresponding diffusivity is unaffected by purity or hydroxyl content; however, the ``anomalously low evolution rates'' which are observed at elevated temperatures in ``dry'' (low OH) quartz are much less predominant in ``wet'' quartz. (3) The total hydrogen content is higher in ``wet'' quartz. (4) The chemical exchange of diffusing (interstitial) hydrogen with residual hydrogen does not obey the same rate laws in ``wet'' and ``dry'' fused quartz. Various hydrogen trapping sites are considered in the light of infrared data and a model is proposed which accounts for the diffusion anomaly (2) as well as the exchange rate differences (4).
Enhanced levels of λ Red-mediated recombinants in mismatch repair mutants (2003)

Homologous recombination can be used to generate recombinants on episomes or directly on the Escherichia coli chromosome with PCR products or synthetic single-stranded DNA (ssDNA) oligonucleotides (oligos). Such recombination is possible because bacteriophage lambda-encoded functions, called Red, efficiently recombine linear DNA with homologies as short as 20-70 bases. This technology, termed recombineering, provides ways to modify genes and segments of the chromosome as well as to study homologous recombination mechanisms. The Red Beta function, which binds and anneals ssDNA to complementary ssDNA, is able to recombine 70-base oligos with the chromosome. In E. coli, methyl-directed mismatch repair (MMR) can affect these ssDNA recombination events by eliminating the recombinant allele and restoring the original sequence. In so doing, MMR can reduce the apparent recombination frequency by >100-fold. In the absence of MMR, Red-mediated oligo recombination can incorporate a single base change into the chromosome in an unprecedented 25% of cells surviving electroporation. Our results show that Beta is the only bacteriophage function required for this level of recombination and suggest that Beta directs the ssDNA to the replication fork as it passes the target sequence.
Packed-capillary liquid chromatography/ion-spray tandem mass spectrometry determination of biomolecules (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPacked-capillary liquid chromatography/ion-spray tandem mass spectrometry determination of biomoleculesEric C. Huang and Jack D. HenionCite this: Anal. Chem. 1991, 63, 7, 732–739Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April 1991https://pubs.acs.org/doi/10.1021/ac00007a016https://doi.org/10.1021/ac00007a016research-articleACS PublicationsRequest reuse permissionsArticle Views127Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Secure and compromise-resilient architecture for advanced metering infrastructure (2011)

In recent years, the Smart Grid has grown to be the solution for future electrical energy that promises to avoid blackouts as well as to be energy efficient, environmentally and customer-friendly. In Smart Grid, the customer-friendly applications are a key element that provides the feature for recognizing the active expenditure of current energy via an Advanced Metering Infrastructure (AMI) subsystem. In fact, the smart meter, as a major part of AMI that is installed in residences, which provides more details about a consumer???s usage. The smart meter measures hour-by-hour usage of a house, and then instantly transmits the record to the utility via two-way communications, unlike the previous electrical system that collects all usage monthly. However, the live measurement of the usage creates a potential privacy leak since each electrical usage records the behaviour of consumers in the home. Therefore, any communication channel between customers and utility should have some sort of confidentiality which protects consumer privacy. In reality, smart meters are generally located in an insecure area of the house (outside), therefore anyone can potentially tamper with the device, noting the fact that it is low-end device. As a result, there is a great possibility of compromising the smart meter, resulting in disclosure of consumer usage. Actually, the nature of a smart meter, and the cost constraints, create a challenge to secure the network. Therefore, the dual motivating problems are the protection of consumer privacy as well as achieving cost efficiency. In this research, we propose a new secure and compromise resilient architecture that continues two major components: a smart meters compromise attack detection scheme and a secure usage reporting protocol. Firstly, the smart meters compromise attack detection scheme improves the security of the smart meter, preventing an adversary from compromising the smart meter. Secondly, the secure usage reporting protocol improves the security of communication between the smart meter and the utility, preventing an adversary from identifying each household's usage reported by smart meters.