gebhart/patrepeval huggingface dataset:
Assessment of Vasoactive Agents and Vascular Aging by the Second Derivative of Photoplethysmogram Waveform (1998)

To evaluate the clinical application of the second derivative of the fingertip photoplethysmogram waveform, we performed drug administration studies (study 1) and epidemiological studies (study 2). In study 1, ascending aortic pressure was recorded simultaneously with the fingertip photoplethysmogram and its second derivative in 39 patients with a mean+/-SD age of 54+/-11 years. The augmentation index was defined as the ratio of the height of the late systolic peak to that of the early systolic peak in the pulse. The second derivative consists of an a, b, c, and d wave in systole and an e wave in diastole. Ascending aortic pressure increased after injection of 2.5 microg angiotensin from 126/74 to 160/91 mm Hg and decreased after 0.3 mg sublingual nitroglycerin to 111/73 mm Hg. The d/a, the ratio of the height of the d wave to that of the a wave, decreased after angiotensin from -0.40+/-0.13 to -0.62+/-0.19 and increased after nitroglycerin to -0.25+/-0.12 (P<0.001 and P<0.001, respectively). The negative d/a increased with increases in plethysmographic and ascending aortic augmentation indices (r=0.79, P<0.001, and r=0.80, P<0.001, respectively). The negative d/a reflects the late systolic pressure augmentation in the ascending aorta and may be useful for noninvasive evaluation of the effects of vasoactive agents. In study 2, the second derivative of the plethysmogram waveform was measured in a total of 600 subjects (50 men and 50 women in each decade from the 3rd to the 8th) in our health assessment center. The b/a ratio increased with age, and c/a, d/a, and e/a ratios decreased with age. Thus, the second derivative aging index was defined as b-c-d-e/a. The second derivative wave aging index (y) increased with age (x) (r=0.80, P<0.001, y=0.023x-1.515). The second derivative aging index was higher in 126 subjects with any history of diabetes mellitus, hypertension, hypercholesterolemia, and ischemic heart disease than in age-matched subjects without such a history (-0.06+/-0.36 versus -0.22+/-0.41, P<0.01). Women had a higher aging index than men (P<0.01). The b-c-d-e/a ratio may be useful for evaluation of vascular aging and for screening of arteriosclerotic disease.
Bounding the Number of Geometric Permutations Induced byk-Transversals (1996)

We prove that a suitably separated family ofncompact convex sets inRdcan be met byk-flat transversals in at mostO(k)d2<//SUP> ((2k+1−2<//SUP>k)(nk+1))k(d−k), or for fixedkandd,O(nk(k+1)(d−k)) different order types. This is the first non-trivial upper bound for 1<k<d−1, and generalizes (asymptotically) the best upper bounds known for line transversals inR,d>2.
Social Network Effects on the Transmission of Sexually Transmitted Diseases (2002)

Among people who had fewer than 13 lifetime sex partners, those with no social friends were only 0.4 times as likely to be infected as those with one or more social friends. Among people who had 13 or more lifetime partners, those with many friends (5 or 6) but weak ties to them (talking less than once a day with them) were only 0.2 times as likely to be infected as those with either fewer friends or stronger ties to their friends. Additional regressions suggest that network effects resting on dyadic control prevail among those with fewer than 13 lifetime sex partners. In contrast, network effects resting on information exchange among third parties prevail among those with 13 or more lifetime partners.
Affinity of human IgG subclasses to mouse Fc gamma receptors (2017)

Human IgG is the main antibody class used in antibody therapies because of its efficacy and longer half-life, which are completely or partly due to FcγR-mediated functions of the molecules. Preclinical testing in mouse models are frequently performed using human IgG, but no detailed information on binding of human IgG to mouse FcγRs is available. The orthologous mouse and human FcγRs share roughly 60-70% identity, suggesting some incompatibility. Here, we report binding affinities of all mouse and human IgG subclasses to mouse FcγR. Human IgGs bound to mouse FcγR with remarkably similar binding strengths as we know from binding to human ortholog receptors, with relative affinities IgG3>IgG1>IgG4>IgG2 and FcγRI>>FcγRIV>FcγRIII>FcγRIIb. This suggests human IgG subclasses to have similar relative FcγR-mediated biological activities in mice.
Depression of endothelial and smooth muscle cell oxygen consumption by endotoxin (1998)

An optical method based on the oxygen-dependent quenching of a phosphorescent probe (palladium-porphyrin) was used to investigate the effect of bacterial endotoxin [lipopolysaccharide (LPS)] on oxygen consumption (VO2) by vascular cells. Endothelial (EC) and smooth muscle (SMC) cells from pig aorta were suspended in culture medium in the presence of palladium-porphyrin and transferred to glass capillary tubes that were sealed to create a hypoxic environment. Measured PO2 changed as a function of time in a highly predictable fashion when cell suspensions were exposed to agents or treatment known to affect cellular metabolism. Both EC and SMC showed a significant decrease in VO2 as cell density increased, and SMC VO2 was significantly higher than EC (1.94 +/- 0.09 vs. 1.0 +/- 0.15 nmol . min-1 . 10(6) cells-1). Exposure to LPS (1 microg/ml) caused a decrease in VO2 of 46% and 15% for EC and SMC, respectively. Pretreatment of cells with N-acetyl-L-cysteine, a substrate for glutathione synthesis with antioxidant properties, restored VO2 to normal values after exposure to LPS. These data suggest that endotoxin impairs VO2 in cells derived from the vascular wall and indicate the importance of EC and SMC respiration in maintaining vascular homeostasis under conditions of sepsis.
Advances in Polymerase Chain Reaction on Microfluidic Chips (2005)

ADVERTISEMENT RETURN TO ISSUEPREVReviewAdvances in Polymerase Chain Reaction on Microfluidic ChipsMichael G. Roper, Christopher J. Easley, and James P. LandersView Author Information Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, and Department of Pathology, University of Virginia Health Science Center, Charlottesville, Virginia 22908 Cite this: Anal. Chem. 2005, 77, 12, 3887–3894Publication Date (Web):May 19, 2005Publication History Published online19 May 2005Published inissue 1 June 2005https://pubs.acs.org/doi/10.1021/ac050756mhttps://doi.org/10.1021/ac050756mreview-articleACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views1803Altmetric-Citations135LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluid dynamics,Genetics,Genomics,Microchips Get e-Alerts
Ablation of Papillar Cell Function in Brassica Flowers Results in the Loss of Stigma Receptivity to Pollination (1993)

Muthugapatti K. Kandasamy, Mary K. Thorsness, Sabine J. Rundle, Michael L. Goldberg, June B. Nasrallah, Mikhail E. Nasrallah, Ablation of Papillar Cell Function in Brassica Flowers Results in the Loss of Stigma Receptivity to Pollination, The Plant Cell, Vol. 5, No. 3 (Mar., 1993), pp. 263-275
Neuronal NADPH diaphorase is a nitric oxide synthase. (1991)

NADPH diaphorase histochemistry selectively labels a number of discrete populations of neurons throughout the nervous system. This simple and robust technique has been used in a great many experimental and neuropathological studies; however, the function of this enzyme has remained a matter of speculation. We, therefore, undertook to characterize this enzyme biochemically. With biochemical and immunochemical assays, NADPH diaphorase was purified to apparent homogeneity from rat brain by affinity chromatography and anion-exchange HPLC. Western (immunoblot) transfer and immunostaining with an antibody specific for NADPH diaphorase labeled a single protein of 150 kDa. Nitric oxide synthase was recently shown to be a 150-kDa, NADPH-dependent enzyme in brain. It is responsible for the calcium/calmodulin-dependent synthesis of the guanylyl cyclase activator nitric oxide from L-arginine. We have found that nitric oxide synthase activity and NADPH diaphorase copurify to homogeneity and that both activities could be immunoprecipitated with an antibody recognizing neuronal NADPH diaphorase. Furthermore, nitric oxide synthase was competitively inhibited by the NADPH diaphorase substrate, nitro blue tetrazolium. Thus, neuronal NADPH diaphorase is a nitric oxide synthase, and NADPH diaphorase histochemistry, therefore, provides a specific histochemical marker for neurons producing nitric oxide.
Wetting Behavior of Water and Oil Droplets in Three-Phase Interfaces for Hydrophobicity/philicity and Oleophobicity/philicity (2009)

Biomimetics, mimicking nature for engineering solutions, provides a model for the development of superhydrophobic/superoleophobic and self-cleaning surfaces. A number of biomimetic superhydrophobic surfaces have been developed by using a hydrophobic coating, surface roughness, and the ability to form air pockets between solid and water. Oleophobic surfaces that have the potential for self-cleaning and antifouling from biological and organic contaminants in both air and water need to be studied. The surface tension of oil and organic liquids is lower than that of water, so to create a superoleophobic surface, the surface energy of the solid surface in air should be lower than that of oil. The wetting behavior of water and oil droplets for hydrophobic/philic and oleophobic/philic surfaces in three-phase interfaces was studied. In order to make the surface oleophobic at a solid-air-oil interface, a material with a surface energy lower than that of oil was used. In underwater applications, the oleophobicity/philicity of an oil droplet in water was studied on the surfaces with different surface energies of various interfaces and contact angles of water and oil droplets in air. A model for predicting the contact angles of water and oil droplets was proposed. To validate the model, the wetting behavior of flat and micropatterned surfaces with varying pitch values were studied. Furthermore, the wetting behavior of the nano- and hierarchical structures found in Lotus plant surfaces and the shark skin replica as an example of aquatic animal were also studied. On the basis of the experimental data and the model, the trends were explained.
Sclerostin Is a Novel Secreted Osteoclast-derived Bone Morphogenetic Protein Antagonist with Unique Ligand Specificity (2003)

Sclerosteosis is a progressive sclerosing bone dysplasia. Sclerostin (the SOST gene) was originally identified as the sclerosteosis-causing gene. However, the physiological role of sclerostin remains to be elucidated. Sclerostin was intensely expressed in developing bones of mouse embryos. Punctuated expression of sclerostin was localized on the surfaces of both intramembranously forming skull bones and endochondrally forming long bones. Sclerostin-positive cells were identified as osteoclasts. Recombinant sclerostin protein produced in cultured cells was efficiently secreted as a monomer. We examined effects of sclerostin on the activity of BMP2, BMP4, BMP6, and BMP7 for mouse preosteoblastic MC3T3-E1 cells. Sclerostin inhibited the BMP6 and BMP7 activity but not the BMP2 and BMP4 activity. Sclerostin bound to BMP6 and BMP7 with high affinity but bound to BMP2 and BMP4 with lower affinity. In conclusion, sclerostin is a novel secreted osteoclast-derived BMP antagonist with unique ligand specificity. We suggest that sclerostin negatively regulates the formation of bone by repressing the differentiation and/or function of osteoblasts induced by BMPs. Since sclerostin expression is confined to the bone-resorbing osteoclast, it provides a mechanism whereby bone apposition is inhibited in the vicinity of resorption. Our findings indicate that sclerostin plays an important role in bone remodeling and links bone resorption and bone apposition.
Virtual path analysis of selective RAKE receiver in dense multipath channels (1999)

We develop an analytical framework to quantify the effects of spreading bandwidth on spread-spectrum systems operating in dense multipath environments. Closed-form expressions for the mean and variance of the total RAKE receiver output signal-to-noise ratio are derived. The proposed problem is made analytically tractable by transforming the physical RAKE paths into the virtual path domain.
Long-term Follow-up Evaluation of a Few Patients with Spondylolisthesis Treated by Excision of the Loose Lamina with Decompression of the Nerve Roots Without Spinal Fusion (1984)

Long-term follow-up observations were recorded in 52 patients with spondylolisthesis who were treated by excision of the loose lamina with decompression of the nerve roots without spinal fusion. The longest follow-up period was 146 months (average, 71 months). Measured by the method of Taillard, the average postoperative increase in displacement in all of the patients, including children, was 5%. In patients with increasing slippage, results were satisfactory in 90% and failures in 10%, whereas 82.7% of the results were satisfactory and 17.3% were failures in the group that showed no progression. Five patients underwent long-term follow-up examinations with roentgenographic studies. Follow-up periods ranged from 17 to 32 years. The largest total displacement was 14%, while only 9% occurred in the other patients. It is obvious, even in this small series, that as the disc degenerates, forward displacement occurs; however, once the disc collapses, further displacement does not occur. The study also revealed that forward displacement does not necessarily cause symptoms. Despite the unusual appearance of recent roentgenograms, the five patients were asymptomatic and satisfied with the end results at last follow-up evaluation.
Palladium-catalyze Arylation of Furan, Thiophene, Benzo[b]furan and Benzo[b]thiophene (1990)

Treatment of π-electron sufficient aromatic heterocycles such as title compounds with aryl bromides in the presence of tetrakis(triphenylphosphine)palladium gave the corresponding 2-aryl aromatic heterocycles.
A novel clinical entity, IgG4-related disease (IgG4RD): general concept and details (2011)

IgG4-related disease (IgG4RD) is a novel clinical disease entity characterized by elevated serum IgG4 concentration and tumefaction or tissue infiltration by IgG4-positive plasma cells. IgG4RD may be present in a certain proportion of patients with a wide variety of diseases, including Mikulicz's disease, autoimmune pancreatitis, hypophysitis, Riedel thyroiditis, interstitial pneumonitis, interstitial nephritis, prostatitis, lymphadenopathy, retroperitoneal fibrosis, inflammatory aortic aneurysm, and inflammatory pseudotumor. Although IgG4RD forms a distinct, clinically independent disease category and is attracting strong attention as a new clinical entity, many questions and problems still remain to be elucidated, including its pathogenesis, the establishment of diagnostic criteria, and the role of IgG4. Here we describe the concept of IgG4RD and up-to-date information on this emerging disease entity.
Curtius rearrangement in the 5‐phenyl‐1,4‐benzodiazepine series. Unprecedented participation by an imine nitrogen (1990)

Abstract The previously unknown 3‐aminomethyl‐1,3‐dihydro‐5‐(2′‐fluorophenyl)‐2 H ,4‐benzodiazepin‐2‐one, 3a, was synthesized in two steps as a racemate. In the chiral series, 3( S )‐azidocarbonylmethyl‐1,3‐dihydro‐5‐phenyl‐2 H ‐1,4‐benzodiazepin‐2‐one, 12b, was prepared from N α‐Cbz‐β‐methylaspartate in five synthetic operations and subjected to Curtius rearrangement. The intermediate isocyanate was trapped intramolecularly by the 5‐imine nitrogen of the benzodiazepine ring in 12b. This unanticipated result runs counter to the generally held dictum that the isocyanate group has a strictly linear shape.
Combining G‐Quadruplex Targeting Motifs on a Single Peptide Nucleic Acid Scaffold: A Hybrid (3+1) PNA–DNA Bimolecular Quadruplex (2008)

We describe the first G-quadruplex targeting approach that combines intercalation and hybridization strategies by investigating the interaction of a G-rich peptide nucleic acid (PNA) acridone conjugate 1 with a three-repeat fragment of the human telomere G 3 to form a hybrid PNA-DNA quadruplex that mimicks the biologically relevant (3+1) pure DNA dimeric telomeric quadruplex. Using a combination of UV and fluorescence spectroscopy, circular dichroism (CD), and mass-spectrometry, we show that PNA 1 can induce the formation of a bimolecular hybrid quadruplex even at low salt concentration upon interaction with a single-stranded three-repeat fragment of telomeric DNA. However, PNA 1 cannot invade a short fragment of B-DNA even if the latter contains a CCC motif complementary to the PNA sequence. These studies could open up new possibilities for the design of a novel generation of quadruplex ligands that target not only the external features of the quadruplex but also its central core constituted by the tetrads themselves.
Determination of halfcystine in proteins as cysteine from reducing hydrolyzates. (1993)

The separation of cysteine from proline using a variant of the classical cation exchange system for amino acid analysis is described. From reducing hydrolyzates (6 M HCl/0.1% phenol/5% thioglycollic acid, 18 h, 110 degrees C) the halfcystine content of proteins can be accurately determined as cysteine. The system is useful for routine determination of the composition of proteins from a single type of hydrolyzate.
Neuropathological changes in scrapie and Alzheimer's disease are associated with increased expression of apolipoprotein E and cathepsin D in astrocytes (1991)

With the rationale that the neuropathological similarities between scrapie and Alzheimer's disease reflect convergent pathological mechanisms involving altered gene expression, we set out to identify molecular events involved in both processes, using scrapie as a model to study the time course of these changes. We differentially screened a cDNA library constructed from scrapie-infected mice to identify mRNAs that increase or decrease during disease and discovered in this way two mRNAs that are increased in scrapie and Alzheimer's disease. These mRNAs were subsequently shown by sequence analysis to encode apolipoprotein E and cathepsin D (EC 3.4.23.5). Using in situ hybridization and immunocytochemistry to define the cellular and anatomic pathology of altered gene expression, we found that in both diseases the increase in apolipoprotein E and cathepsin D mRNAs and proteins occurred in activated astrocytes. In scrapie, the increase in gene expression occurred soon after the amyloid-forming abnormal isoform of the prion protein has been shown to accumulate in astrocytes. In Alzheimer's disease, the increased expression of cathepsin D also occurred in association with beta-amyloid. These studies reveal some of the molecular antecedents of neuropathological changes in scrapie and Alzheimer's disease and accord new prominence to the role of astrocytes in neurodegenerative conditions.
Discovery of Isoxazole Analogues of Sazetidine-A as Selective α4β2-Nicotinic Acetylcholine Receptor Partial Agonists for the Treatment of Depression (2011)

Depression, a common neurological condition, is one of the leading causes of disability and suicide worldwide. Standard treatment, targeting monoamine transporters selective for the neurotransmitters serotonin and noradrenaline, is not able to help many patients that are poor responders. This study advances the development of sazetidine-A analogues that interact with α4β2 nicotinic acetylcholine receptors (nAChRs) as partial agonists and that possess favorable antidepressant profiles. The resulting compounds that are highly selective for the α4β2 subtype of nAChR over α3β4-nAChRs are partial agonists at the α4β2 subtype and have excellent antidepressant behavioral profiles as measured by the mouse forced swim test. Preliminary absorption, distribution, metabolism, excretion, and toxicity (ADMET) studies for one promising ligand revealed an excellent plasma protein binding (PPB) profile, low CYP450-related metabolism, and low cardiovascular toxicity, suggesting it is a promising lead as well as a drug candidate to be advanced through the drug discovery pipeline.
Next-generation tag sequencing for cancer gene expression profiling (2009)

We describe a new method, Tag-seq, which employs ultra high-throughput sequencing of 21 base pair cDNA tags for sensitive and cost-effective gene expression profiling. We compared Tag-seq data to LongSAGE data and observed improved representation of several classes of rare transcripts, including transcription factors, antisense transcripts, and intronic sequences, the latter possibly representing novel exons or genes. We observed increases in the diversity, abundance, and dynamic range of such rare transcripts and took advantage of the greater dynamic range of expression to identify, in cancers and normal libraries, altered expression ratios of alternative transcript isoforms. The strand-specific information of Tag-seq reads further allowed us to detect altered expression ratios of sense and antisense (S-AS) transcripts between cancer and normal libraries. S-AS transcripts were enriched in known cancer genes, while transcript isoforms were enriched in miRNA targeting sites. We found that transcript abundance had a stronger GC-bias in LongSAGE than Tag-seq, such that AT-rich tags were less abundant than GC-rich tags in LongSAGE. Tag-seq also performed better in gene discovery, identifying >98% of genes detected by LongSAGE and profiling a distinct subset of the transcriptome characterized by AT-rich genes, which was expressed at levels below those detectable by LongSAGE. Overall, Tag-seq is sensitive to rare transcripts, has less sequence composition bias relative to LongSAGE, and allows differential expression analysis for a greater range of transcripts, including transcripts encoding important regulatory molecules.
Transformation Properties of Learned Visual Representations (2014)

When a three-dimensional object moves relative to an observer, a change occurs on the observer's image plane and in the visual representation computed by a learned model. Starting with the idea that a good visual representation is one that transforms linearly under scene motions, we show, using the theory of group representations, that any such representation is equivalent to a combination of the elementary irreducible representations. We derive a striking relationship between irreducibility and the statistical dependency structure of the representation, by showing that under restricted conditions, irreducible representations are decorrelated. Under partial observability, as induced by the perspective projection of a scene onto the image plane, the motion group does not have a linear action on the space of images, so that it becomes necessary to perform inference over a latent representation that does transform linearly. This idea is demonstrated in a model of rotating NORB objects that employs a latent representation of the non-commutative 3D rotation group SO(3).