gebhart/patrepeval huggingface dataset:
Mechanisms of vasomotor rhinitis (2004)

Nonallergic non-infectious perennial rhinitis (NANIPER) is a heterogeneous disorder comprising several pathophysiological entities. The etiology of some of these disorders (e.g. drug-induced rhinitis, nonallergic rhinitis with eosinophilia syndrome [NARES], occupational rhinitis, hormonal rhinitis, emotion-induced rhinitis, physical/chemical irritant-induced rhinitis) is well established. In contrast, the aetiology of idiopathic forms of rhinitis (also known as vasomotor rhinitis) is largely unknown. Mechanistic studies have suggested that non-IgE-mediated inflammatory and/or neurogenic processes may be involved. There is evidence that localized inflammation is the underlying cause of symptoms in drug-induced rhinitis and NARES, since eosinophilia is an important pathophysiological component in these conditions. In contrast, neurogenic reflex mechanisms initiated by environmental factors appear to be involved in idiopathic rhinitis. It has been suggested that there may be an imbalance of the sympathetic and parasympathetic nervous systems, with parasympathetic hyper-activity and sympathetic hypo-activity resulting in nasal congestion and rhinorrhoea. Indirect evidence suggests that C-fibres may also play an important role in the pathophysiology of idiopathic rhinitis.
Targeting the JMJD2 Histone Demethylases to Epigenetically Control Herpesvirus Infection and Reactivation from Latency (2013)

Chromatin and the chromatin modulation machinery not only provide a regulatory matrix for enabling cellular functions such as DNA replication and transcription but also regulate the infectious cycles of many DNA viruses. Elucidation of the components and mechanisms involved in this regulation is providing targets for the development of new antiviral therapies. Initiation of infection by herpes simplex virus (HSV) requires the activity of several cellular chromatin modification enzymes including the histone demethylases LSD1 and the family of JMJD2 proteins that promote transcriptional activation of the initial set of viral genes. Depletion of the JMJD2 members or inhibition of their activity with a new drug results in repression of expression of viral immediate early genes and abrogation of infection. This inhibitor also represses the reactivation of HSV from the latent state in sensory neurons. Like HSV, the β-herpesvirus human cytomegalovirus also requires the activity of LSD1 and the JMJD2s to initiate infection, thus demonstrating the potential of this chromatin-based inhibitor to be useful against a variety of different viruses.
Population Genetics in Forensic DNA Typing (1991)

Variable number of tandem repeat (VNTR) sequences are used to link defendants with crimes by matching DNA patterns. The probative value of a match is often calculated by multiplying together the estimated frequencies with which each particular VNTR pattern occurs in a reference database. However, this method is liable to potentially serious errors because ethnic subgroups within major racial categories exhibit genetic differences that are maintained by endogamy. The multiplication procedure currently in use can be made scientifically valid only by extensive sampling of VNTR frequency distributions in a variety of ethnic groups, similar to the ethnic studies of various blood groups done in the past. Alternative approaches for dealing with subpopulation heterogeneity are discussed.
Antigen-Specific IL-2 Secretion Correlates with NK Cell Responses after Immunization of Tanzanian Children with the RTS,S/AS01 Malaria Vaccine (2012)

RTS,S/AS01, a vaccine targeting pre-erythrocytic stages of Plasmodium falciparum, is undergoing clinical trials. We report an analysis of cellular immune response to component Ags of RTS,S-hepatitis B surface Ag (HBs) and P. falciparum circumsporozoite (CS) protein-among Tanzanian children in a phase IIb RTS,S/AS01(E) trial. RTS,S/AS01 (E) vaccinees make stronger T cell IFN-γ, CD69, and CD25 responses to HBs peptides than do controls, indicating that RTS,S boosts pre-existing HBs responses. T cell CD69 and CD25 responses to CS and CS-specific secreted IL-2 were augmented by RTS,S vaccination. Importantly, more than 50% of peptide-induced IFN-γ(+) lymphocytes were NK cells, and the magnitude of the NK cell CD69 response to HBs peptides correlated with secreted IL-2 concentration. CD69 and CD25 expression and IL-2 secretion may represent sensitive markers of RTS,S-induced, CS-specific T cells. The potential for T cell-derived IL-2 to augment NK cell activation in RTS,S-vaccinated individuals, and the relevance of this for protection, needs to be explored further.
DNA binding cationic bolasomes with delocalized charge center - A structure-activity relationship study (2001)

Bolaamphiphilic dicationic quinolinium derivatives are being explored by us for the development of mitochondria-specific drug and DNA delivery systems. In this structure-activity relationship study, defined connections between the structure of the bolaamphiphiles and their ability to form cationic vesicles (bolasomes) and to bind DNA are established. Nine quinolinium derivatives with varying polar head groups and hydrophobic tail segments were synthesized according to published procedures. Their self-assembly behavior was determined by photon correlation spectroscopy and their affinity to DNA was measured using the fluorescence dye SYBR Green I. The introduction of a DNA binding capacity allows the quantitative comparison between the quinolinium derivatives and Lipofectin regarding their ability to form lipoplexes. It is demonstrated that the primary 4-amino group at the quinolinium ring system plays an important role in DNA binding. The presence of a bulky aliphatic residue at the aromatic ring system favors the self-assembly of these bolaamphiphiles into bolasomes
Gene Manipulation and Transfer of the Plasminogen and Coagulation System in Mice (1996)

The blood coagulation and the fibrinolytic (or plasminogen/plasmin) systems determine the balance between the formation and dissolution of blood clots, but in addition contribute to the pathogenesis of various cardiovascular disorders such as thrombosis, atherosclerosis, and restenosis. Furthermore, they participate in a variety of other (patho)biological processes such as embryonic development, reproduction, wound healing, cancer, and brain function. Two recently developed technologies, gene targeting and gene transfer, that allow manipulation of the genetic balance of these proteinase systems in a controllable manner have allowed a more definitive elucidation of the biological role of these systems. This review summarizes the insights that have been obtained from the gene targeting studies and discusses the use of adenovirus-mediated transfer of fibrinolytic genes to study and possibly to develop novel strategies for the treatment of restenosis and thrombosis.
Regulation of Vascular Endothelial Growth Factor Expression in Cultured Keratinocytes. (1995)

Recent in situ hybridization studies had demonstrated a strong increase in vascular endothelial growth factor (VEGF) mRNA expression in the hyperproliferative epithelium during wound healing. To determine potential mediators of VEGF induction during this process, we analyzed the regulation of VEGF expression in cultured human keratinocytes. We found a large induction of VEGF expression upon treatment of quiescent cells with serum, epidermal growth factor, transforming growth factor-beta 1, keratinocyte growth factor, or the proinflammatory cytokine tumor necrosis factor alpha, respectively. Since all these factors are present at the wound site during the early phase of wound healing, they might also be responsible for VEGF induction after cutaneous injury. To determine the importance of increased VEGF production for wound repair, we compared the time course of VEGF mRNA expression during wound healing of healthy control mice with the kinetics of VEGF expression during skin repair of genetically diabetic db/db mice which are characterized by impaired wound healing. In normal mice we found elevated VEGF mRNA levels during the period when granulation tissue formation occurs. In contrast, VEGF mRNA levels even declined during this period in db/db mice, suggesting that a defect in VEGF regulation might be associated with wound healing disorders.
The 4-Dimensional Rhytidoplasty (2001)

Introduction: The 4-dimensional facelift is an advanced rhytidectomy technique using a combination of the subperiosteal and multiplanar/multivector facelift techniques simultaneously with CO 2 and erbium:YAG laser resurfacing for maximal facial rejuvenation. The combination of these procedures produces a rejuvenation that has synergistic benefits. The facelift alone does not rejuvenate fine rhytids around the mouth and infraorbital regions. In addition, laser resurfacing does not address the rhytids caused by deeper ptotic anatomic structures, nor lines of animation. The 4-dimensional rhytidoplasty provides the ultimate in subtle release of the anatomic facial planes. This concentric release of the various anatomic planes reduces unnecessary tension and unnecessary vascular compromise on any particular layer. This results in increased safety of the technique, allows for simultaneous laser resurfacing, and gives a more natural and improved facial rejuvenation. Materials and Methods: Thirty-two patients (4 males and 28 females) underwent the 4-dimensional facelift between November 1998 and December 1999. This includes simultaneous laser resurfacing and a deep-plane multivector facelift, including an extended subperiosteal release. After all adjunctive procedures had been performed and the superficial musculoaponeurotic system had been stabilized, the redundant preauricular skin was lasered, keeping the energy densities the same, but altering the number of passes. The edges were then trimmed and submitted for histologic examination. Conclusions: The authors are not aware of any previous publications combining all the anatomic planes of the face into a single operation. It has also been the accepted dictum by various experts in the field that laser resurfacing over the undermined flap was a contraindication. However, on the basis of our experiences and results, it is our opinion that this can be done and that the clinical endpoint is safely achieved, resulting in a superior cosmetic result and an assumed increased longevity.
Wideband Integrated Voice/Data Technology (1983)

Abstract : An important challenge in the design of future military communications networks is to achieve overall system economy and adaptability through efficient and flexible allocation of common network resources to voice and data users. The major objective of the Wideband Integrated Voice/Data Technology Program is to address this challenge through the development of techniques for integrated voice and data communications in digital packet networks which include wideband common user satellite links. This report covers work in the following areas: development of a generalized compact vocoder based on digital LSI technology; development of Packet Voice Terminal (PVT) and local access network (LEXNET) facilities for experiments in packet voice; development and experimental application of a flexible internet stream gateway (the miniconcentrator) for packet voice; coordination and execution of multi-user packet speech experiments on the experimental wideband satellite network (WB SATNET); and development and experimental testing of a Voice-Controlled Operator (VCOP).
Suzuki—Miyaura Cross‐Coupling Reactions of Benzyl Halides with Potassium Aryltrifluoroborates. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Inhibition of neointimal proliferation in rabbits after vascular injury by a single treatment with a protein adduct of nitric oxide. (1995)

Endothelium-derived relaxing factor is important for vascular homeostasis and possesses qualities that may modulate vascular injury, including vasodilation, platelet inhibition, and inhibition of smooth muscle proliferation. S-nitrososerum albumin is a naturally occurring adduct of nitric oxide (NO) with a prolonged biologic half-life and is a potent vasodilator and platelet inhibitor. Given the avidity of serum albumin for subendothelial matrix and the antiproliferative effects of NO, we investigated the effects of locally delivered S-nitroso-bovine serum albumin (S-NO-BSA) and a polythiolated form of bovine serum albumin (pS-BSA) modified to carry several S-nitrosothiol groups (pS-NO-BSA) on neointimal responses in an animal model of vascular injury. Locally delivered S-NO-BSA bound preferentially to denuded rabbit femoral vessels producing a 26-fold increase in local concentration compared with uninjured vessels (P = 0.029). pS-NO-BSA significantly reduced the intimal/medial ratio (P = 0.038) and did so in conjunction with elevations in platelet (P < 0.001) and vascular cGMP content (P < or = 0.001). pS-NO-BSA treatment also inhibited platelet deposition (P = 0.031) after denuding injury. Comparison of BSA, S-NO-BSA, pS-NO-BSA, and control revealed a dose-response relationship between the amount of displaceable NO delivered and the extent of inhibition of neointimal proliferation at 2 wk (P < or = 0.001). Local administration of a stable protein S-nitrosothiol inhibits intimal proliferation and platelet deposition after vascular arterial balloon injury. This strategy for the local delivery of a long-lived NO adduct has potential for preventing restenosis after angioplasty.
Design, fabrication, and packaging of a practical multianalyte-capable optical biosensor (2006)

A unique evanescent-wave biosensor was designed and fabricated using simple and robust microfabrication technology. The sensor uses a microscale optical waveguide fabricated from NOA61 that is surface-altered with a custom chemical modification process, coupled with modified self-assembly of an oxygen-sensitive fluorescent dye and the enzyme glucose oxidase. To interface the analyte with the waveguide surface, a multilayer PDMS fluidic mold was designed to fit over the waveguide. Interfacing of both optics and fluidics was achieved using novel-generic methods. The entire device has been successfully fabricated and assembled, with analyte response testing completed using glucose solutions. The system also demonstrated inherent random noise insensitivity while making measurements.
Chemotherapy-induced alopecia in mice. Induction by cyclophosphamide, inhibition by cyclosporine A, and modulation by dexamethasone. (1994)

We introduce cyclophosphamide-induced alopecia (CYP-IA) in C57BL-6 mice as a clinically relevant model for studying the biology of chemotherapy-induced alopecia and for developing anti-alopecia drugs. One injection of CYP to mice with all back skin follicles in anagen VI induces severe alopecia that strikingly reproduces the follicle response, recovery, and histopathology seen in human CYP-IA. CYP dose-dependently induces abnormal follicular melanogenesis and dystrophic anagen or, in more severely damaged follicles, dystrophic catagen. Both dystrophy forms are followed by an extremely shortened telogen phase, but differ in the associated hair loss and in recovery patterns, which determines hair regrowth. This follicular response to CYP can be manipulated pharmacologically: systemic cyclosporine A shifts it toward a mild form of dystrophic anagen, thus retarding CYP-IA and prolonging "primary recovery". Topical dexamethasone, in contrast, forces follicles into dystrophic catagen, which augments CYP-IA, but accelerates the regrowth of normally pigmented hair ("secondary recovery").
Optimal ultraviolet wavelength for <italic>in vivo</italic> photoacoustic imaging of cell nuclei (2012)

In order to image noninvasively cell nuclei in vivo without staining, we have developed ultraviolet photoacoustic microscopy (UV-PAM), in which ultraviolet light excites nucleic acids in cell nuclei to produce photoacoustic waves. Equipped with a tunable laser system, the UV-PAM was applied to in vivo imaging of cell nuclei in small animals. We found that 250 nm was the optimal wavelength for in vivo photoacoustic imaging of cell nuclei. The optimal wavelength enables UV-PAM to image cell nuclei using as little as 2 nJ laser pulse energy. Besides the optimal wavelength, application of a wavelength between 245 and 275 nm can produce in vivo images of cell nuclei with specific, positive, and high optical contrast.
Modeling and design of planar slanted volume holographic gratings for wavelength-division-multiplexing applications (1999)

Holographic gratings are modeled and designed for path-reversed substrate-guided-wave wavelength-division demultiplexing (WDDM) as a continuation of earlier research [Appl. Opt. 38, 3046 (1999)]. An efficient and practical method is developed to simulate the slanted volume holographic gratings. The trade-off between dispersion and the bandwidth of the holograms is analyzed. A 60 degrees (incident angle of the grating)/60 degrees (diffraction angle of the grating in air) grating structure is selected to demultiplex optical signals in the 1555-nm spectral region, and a 45 degrees /45 degrees grating structure is chosen for the spectral region near 800 nm. Experimental results are consistent with the simulation results for these two WDDM devices. A four-channel WDDM is also demonstrated at a center wavelength of 1555 nm and with a channel spacing of 2 nm.
Endothelial sulfonylurea receptor 1–regulated NCCa-ATP channels mediate progressive hemorrhagic necrosis following spinal cord injury (2007)

Acute spinal cord injury (SCI) causes progressive hemorrhagic necrosis (PHN), a poorly understood pathological process characterized by hemorrhage and necrosis that leads to devastating loss of spinal cord tissue, cystic cavitation of the cord, and debilitating neurological dysfunction. Using a rodent model of severe cervical SCI, we tested the hypothesis that sulfonylurea receptor 1-regulated (SUR1-regulated) Ca(2+)-activated, [ATP](i)-sensitive nonspecific cation (NC(Ca-ATP)) channels are involved in PHN. In control rats, SCI caused a progressively expansive lesion with fragmentation of capillaries, hemorrhage that doubled in volume over 12 hours, tissue necrosis, and severe neurological dysfunction. SUR1 expression was upregulated in capillaries and neurons surrounding necrotic lesions. Patch clamp of cultured endothelial cells exposed to hypoxia showed that upregulation of SUR1 was associated with expression of functional SUR1-regulated NC(Ca-ATP) channels. Following SCI, block of SUR1 by glibenclamide or repaglinide or suppression of Abcc8, which encodes for SUR1 by phosphorothioated antisense oligodeoxynucleotide essentially eliminated capillary fragmentation and progressive accumulation of blood, was associated with significant sparing of white matter tracts and a 3-fold reduction in lesion volume, and resulted in marked neurobehavioral functional improvement compared with controls. We conclude that SUR1-regulated NC(Ca-ATP) channels in capillary endothelium are critical to development of PHN and constitute a major target for therapy in SCI.
Response of herbivorous fishes to crown-of-thorns starfish Acanthaster planci outbreaks. I. Substratum analysis and feeding ecology of Acanthurus nigrofuscus and Scarus frenatus (1996)

A large-scale comparative study of 3 crown-of-thorns starflsh (COTS) impacted reefs and 3 non-impacted (control) reefs in the central reglon of the Great Barner Reef was undertaken. The principal objective was to investigate a long-term functional response of hcrbivorous fish to a potentially greater food rcsource in the form of increased abundances of turf algae on COTS affected reefs. Measurement of substratum cover and feeding ecology of Acanthurus njgrofuscus and Scarus frenatus was made on the windward reef slope of all reefs at a depth of 3 to 7 m. Significantly higher abundances of turf algae in con~parison with live coral cove! occurred on COTS nffected reefs, while the opposite pattern occurred on non-affected reefs. However, no obv~ous difference was detected in the overall dietary composition of A . n~grofuscus, variability being more apparent at a temporal scale. There was no difference in feeding rates of rovlng herbivores (2 specles) between impacted and control reefs. Furthermore, one component of the turf algal assemblage (thick turfs) showed increases in abundance in winter on affected reefs. while on non-affected reefs, thick turfs did not exhibit this seasonal pattern. A similar trend was observed for Cyanophyta (blue-green algae) in the dietary assemblage of A. nigrofuscus, although different sampling times and lack of detailed knowledge of epilithic algal community (EAC) composition made direct correlations difficult to infer Whether this increased turf algal resource is prompting a numerical or growth response from herbivorous fishes is curl-ently being investlgated.
Proliferation and Death of Oligodendrocytes and Myelin Proteins Are Differentially Regulated in Male and Female Rodents (2006)

Sexual dimorphism of neurons and astrocytes has been demonstrated in different centers of the brain, but sexual dimorphism of oligodendrocytes and myelin has not been examined. We show, using immunocytochemistry and in situ hybridization, that the density of oligodendrocytes in corpus callosum, fornix, and spinal cord is 20-40% greater in males compared with females. These differences are present in young and aged rodents and are independent of strain and species. Proteolipid protein and carbonic anhydrase-II transcripts, measured by real-time PCR, are approximately two to three times greater in males. Myelin basic protein and 2', 3'-cyclic nucleotide 3'-phosphodiesterase, measured by Western blots, are 20-160% greater in males compared with females. Surprisingly, both generation of new glia and apoptosis of glia, including oligodendrocytes, are approximately two times greater in female corpus callosum. These results indicate that the lifespan of oligodendrocytes is shorter in females than in males. Castration of males produces a female phenotype characterized by fewer oligodendrocytes and increased generation of new glia. These findings indicate that exogenous androgens differentially affect the lifespan of male and female oligodendrocytes, and they can override the endogenous production of neurosteroids. The data imply that turnover of myelin is greater in females than in males. Mu-calpain, a protease upregulated in degeneration of myelin, is dramatically increased at both transcriptional and translational levels in females compared with males. These morphological, molecular, and biochemical data show surprisingly large differences in turnover of oligodendrocytes and myelin between sexes. We discuss the potential significance of these differences to multiple sclerosis, a sexually dimorphic disease, whose progression is altered by exogenous hormones.