gebhart/patrepeval huggingface dataset:
Processing of the Borna Disease Virus Glycoprotein gp94 by the Subtilisin-Like Endoprotease Furin (1998)

Open reading frame IV (ORF-IV) of Borna disease virus (BDV) encodes a protein with a calculated molecular mass of ca. 57 kDa (p57), which increases after N glycosylation to 94 kDa (gp94). The unglycosylated and glycosylated proteins are proteolytically cleaved by the subtilisin-like protease furin. Furin most likely recognizes one of three potential cleavage sites, namely, an arginine at position 249 of the ORF-IV gene product. The furin inhibitor decRVKRcmk decreases the production of infectious BDV significantly, indicating that proteolytic cleavage of the gp94 precursor molecule is necessary for the full biological activity of the BDV glycoprotein.
Particle resuspension rates (1974)

Particle resuspension rates were measured during four ,months of field t e s t i n g i n a l i g h t l y vegetated area.Resuspension of an i-nert submicrometer tracer placed on the ground i n a c i r c l e of 29 m radius was measured.A t the c i r c l e center, airborne concentrations were measured t o heights of 6.1 m. .The resuspended plwne extended above 6.1 m.Resuspension rates were from 10-I t o 10'~ fraction resuspended/sec.Resuspension rates increased non-linearly as a function of wind speed.For wind speeds greater thmr 3.6 m/sec, resuspension rates increased with wind speed t o the go 5 power.
Deposition-related histone acetylation in micronuclei of conjugating Tetrahymena. (1985)

Macro- and micronuclei of the ciliated protozoan, Tetrahymena thermophila, afford a unique opportunity to study histone acetylation under conditions where acetylation associated with the regulation of transcription and acetylation associated with the deposition of histones on the DNA are separable. In this study we demonstrate that histone H3 and histone H4 synthesized in young (5 hr) conjugating Tetrahymena are deposited into micronuclei in acetylated forms. Most of the newly synthesized histone H3 migrates as a monoacetylated form while essentially all of the new histone H4 is deposited as a diacetylated species. Since micronuclei replicate rapidly during this stage of the life cycle, but are transcriptionally inactive, these data suggest that histone acetylation is related functionally to histone deposition and chromatin assembly. Pulse-chase experiments show that micronuclei also contain a butyrate-sensitive deacetylase activity(ies) which operates to remove the deposition-related acetate groups from newly synthesized and deposited H3 and H4. This enzymatic activity probably contributes to the steady state level of micronuclear histone acetylation that is low or nonexistent. Thus, evidence is emerging for at least two independent systems of histone acetylation in Tetrahymena. The first system is specific to macronuclei and may be related to gene expression. The second system is common to macro- or micronuclear histones (H3 and H4) and may be related to histone deposition during DNA replication.
Glass transitions of some block copolymers (1974)

A number of block and graft copolymers have been prepared from several vinyl monomers, and their glass transition temperatures measured. The series included polar, nonpolar and polar-nonpolar pairs. It was found in all cases except one that the glass transition temperatures were close to those found for the individual homopolymers. The exception was a block copolymer of styrene and α-methylstyrene, the only pair whose homopolymers would give clear mixed solutions in a mutual solvent.
Molecular cloning of a novel widely expressed human 80 kDa 17β-hydroxysteroid dehydrogenase IV (1995)

Reactions of oestrogens and androgens at position C-17 are catalysed by 17 beta-hydroxysteroid dehydrogenases (17 beta-HSDs). Cloning of the cDNA of a novel human 17 beta-HSD IV and expression of its mRNA are described. A probe derived from the recently discovered porcine 17 beta-oestradiol dehydrogenase (17 beta-EDH) was used to isolate a 2.6 kb human cDNA encoding a continuous protein of 736 amino acids of high (84%) similarity to the porcine 17 beta-EDH. The calculated molecular mass of the human enzyme is 79,595 Da. Other sequence similarities shared by the two enzymes are: an N-terminal sequence which is similar to that of members of the short-chain alcohol dehydrogenase family; amino acids 343-607 which are similar to the C-terminal domains of a trifunctional Candida tropicalis enzyme and the FOX2 gene product of Saccharomyces cerevisiae; amino acids 596-736 which are similar to human sterol carrier protein 2. The previously cloned human 17 beta-HSD I, II and III are less than 25% identical with 17 beta-HSD IV. mRNA for HSD IV is a single species of 3.0 kb, present in many tissues with highest concentrations in liver, heart, prostate and testes. When over-expressed in mammalian cells, the human 17 beta-HSD IV enzyme displays a specific unidirectional oxidative 17 beta-HSD activity.
Comparison of the Blood Coagulation Profiles of Ferrets and Rats (2011)

The aim of this study was to examine the blood coagulation profiles of ferrets and compare them with those of rats. The ferret activated partial thromboplastin time (aPTT) was slightly longer than the rat aPTT. In contrast, the ferret prothrombin time and thrombin time were profoundly shorter than the corresponding rat values. The fibrinogen level in ferret plasma was 2 times higher than that in rats. Heparin prolonged all blood coagulation times in a concentration-dependent manner in both ferret and rat plasma. A significantly (P<0.01) higher concentration of heparin was required to double the aPTT in ferrets than rats. These blood coagulation data for ferrets will be useful in experimental animal studies.
Application Performance Management in Virtualized Server Environments (2006)

As businesses have grown, so has the need to deploy I/T applications rapidly to support the expanding business processes. Often, this growth was achieved in an unplanned way: each time a new application was needed a new server along with the application software was deployed and new storage elements were purchased. In many cases this has led to what is often referred to as "server sprawl", resulting in low server utilization and high system management costs. An architectural approach that is becoming increasingly popular to address this problem is known as server virtualization. In this paper we introduce the concept of server consolidation using virtualization and point out associated issues that arise in the area of application performance. We show how some of these problems can be solved by monitoring key performance metrics and using the data to trigger migration of virtual machines within physical servers. The algorithms we present attempt to minimize the cost of migration and maintain acceptable application performance levels
Occurrence of brown adipocytes in rat white adipose tissue: molecular and morphological characterization (1992)

Brown adipocytes are thermogenic cells which play an important role in energy balance. Their thermogenic activity is due to the presence of a mitochondrial uncoupling protein (UCP). Until recently, it was admitted that in rodents brown adipocytes were mainly located in classical brown adipose tissue (BAT). In the present study, we have investigated the presence of UCP protein or mRNA in white adipose tissue (WAT) of rats. Using polymerase chain reaction or Northern blot hybridization, UCP mRNA was detected in mesenteric, epidydimal, retroperitoneal, inguinal and particularly in periovarian adipose depots. The uncoupling protein was detected by Western blotting in mitochondria from periovarian adipose tissue. When rats were submitted to cold or to treatment with a beta-adrenoceptor agonist, UCP expression was increased in this tissue as in typical brown fat. Moreover, the expression was decreased in obese fa/fa rats compared to lean controls. Morphological studies showed that periovarian adipose tissue of rats kept at 24 degrees C contained cells with numerous typical BAT mitochondria with or without multilocular lipid droplets. Immunocytochemistry confirmed that multilocular cells expressed mitochondrial UCP. Furthermore, the number of brown adipocytes and the density of mitochondrial cristae increased in parallel with exposure to cold. These results demonstrate that adipocytes expressing UCP are present in adipose deposits considered as white fat. They suggest the existence of a continuum in rodents between BAT and WAT, and a great plasticity between adipose tissue phenotypes. The physiological importance of brown adipocytes in WAT and the regulation of UCP expression remain open questions.
Extracellular vesicles derived from mesenchymal stromal cells: a therapeutic option in respiratory diseases? (2016)

Extracellular vesicles (EVs) are plasma membrane-bound fragments released from several cell types, including mesenchymal stromal cells (MSCs), constitutively or under stimulation. EVs derived from MSCs and other cell types transfer molecules (such as DNA, proteins/peptides, mRNA, microRNA, and lipids) and/or organelles with reparative and anti-inflammatory properties to recipient cells. The paracrine anti-inflammatory effects promoted by MSC-derived EVs have attracted significant interest in the regenerative medicine field, including for potential use in lung injuries. In the present review, we describe the characteristics, biological activities, and mechanisms of action of MSC-derived EVs. We also review the therapeutic potential of EVs as reported in relevant preclinical models of acute and chronic respiratory diseases, such as pneumonia, acute respiratory distress syndrome, asthma, and pulmonary arterial hypertension. Finally, we discuss possible approaches for potentiating the therapeutic effects of MSC-derived EVs so as to enable use of this therapy in clinical practice.
Cholesteryl Oligoarginine Delivering Vascular Endothelial Growth Factor siRNA Effectively Inhibits Tumor Growth in Colon Adenocarcinoma (2006)

Vascular endothelial growth factor (VEGF) is a multifunctional angiogenic growth factor that is a primary stimulant of the development and maintenance of a vascular network in the vascularization of solid tumors. It has been reported that a blockade of VEGF-mediated angiogenesis is a powerful method for tumor regression. RNA interference represents a naturally occurring biological strategy for inhibition of gene expression. In mammalian systems, however, the in vivo application of small interfering RNA (siRNA) is severely limited by the instability and poor bioavailability of unmodified siRNA molecules. In this study, we tested the hypothesis that a hydrophobically modified protein transduction domain, cholesteryl oligo-d-arginine (Chol-R9), may stabilize and enhance tumor regression efficacy of the VEGF-targeting siRNA. The noncovalent complexation of a synthetic siRNA with Chol-R9 efficiently delivered siRNA into cells in vitro. Moreover, in a mouse model bearing a subcutaneous tumor, the local administration of complexed VEGF-targeting siRNA, but not of scrambled siRNA, led to the regression of the tumor. Hence, we propose a novel and simple system for the local in vivo application of siRNA through Chol-R9 for cancer therapy.
A Facile Method for Reversibly Linking a Recombinant Protein to DNA (2009)

We present a facile method for linking recombinant proteins to DNA. It is based on the nickel-mediated interaction between a hexahistidine tag (His(6)-tag) and DNA functionalized with three nitrilotriacetic acid (NTA) groups. The resulting DNA-protein linkage is site-specific. It can be broken quickly and controllably by the addition of a chelating agent that binds nickel. We have used this new linker to bind proteins to a variety of DNA motifs commonly used in the fabrication of nanostructures by DNA self-assembly.
Control of ftsZ Expression, Cell Division, and Glutamine Metabolism in Luria-Bertani Medium by the Alarmone ppGpp in Escherichia coli (1998)

Inactivation of transcription factor sigma54, encoded by rpoN (glnF), restores high-temperature growth in Luria-Bertani (LB) medium to strains containing the heat-sensitive cell division mutation ftsZ84. Mutational defects in three other genes involved in general nitrogen control (glnD, glnG, and glnL) also suppress lethal filamentation. Since addition of glutamine to LB medium fully blocks suppression by each mutation, the underlying cause of suppression likely derives from a stringent response to the limitation of glutamine. This model is supported by several observations. The glnL mutation requires RelA-directed synthesis of the nutrient alarmone ppGpp to suppress filamentation. Artificially elevated levels of ppGpp suppress ftsZ84, as do RNA polymerase mutations that reproduce global effects of the ppGpp-induced state. Both the glnF null mutation and an elevated copy number of the relA gene similarly affect transcription from the upstream (pQ) promoters of the ftsQAZ operon, and both of these genetic conditions increase the steady-state level of the FtsZ84 protein. Physiological suppression of ftsZ84 by a high salt concentration was also shown to involve RelA. Additionally, we found that the growth of a glnF or glnD strain on LB medium depends on RelA or supplemental glutamine in the absence of RelA function. These data expand the roles for ppGpp in the regulation of glutamine metabolism and the expression of FtsZ during cell division.
Significance of Von Willebrand Factor in Septic and Nonseptic Patients with Acute Lung Injury (2004)

Systemic endothelial activation and injury are important causes of multiorgan system failure. We hypothesized that plasma levels of von Willebrand factor (VWF), a marker of endothelial activation and injury, would be associated with clinical outcomes in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). In 559 patients with ALI/ARDS enrolled in the National Heart, Lung, and Blood Institute ARDS Network trial of two VT strategies, plasma VWF levels were measured at randomization (mean 350 +/- 265% of normal control plasma) and Day 3 (344 +/- 207%). Baseline VWF levels were similar in patients with and without sepsis, and were significantly higher in nonsurvivors (435 +/- 333%) versus survivors (306 +/- 209%) even when controlling for severity of illness, sepsis, and ventilator strategy (increased odds ratio of death of 1.6 per SD size increase in VWF; 95% confidence interval, 1.4-2.1). Higher VWF levels were also significantly associated with fewer organ failure-free days. Ventilator strategy had no effect on VWF levels. In conclusion, the degree of endothelial activation and injury is strongly associated with outcomes in ALI/ARDS, regardless of the presence or absence of sepsis, and is not modulated by a protective ventilatory strategy. To improve outcomes further, new treatment strategies targeted at the endothelium should be investigated.
Intravascular Sonotherapy Decreases Neointimal Hyperplasia After Stent Implantation in Swine (2001)

In this swine peripheral model, intravascular sonotherapy seemed to decelerate cellular proliferation and decrease in-stent hyperplasia. Therefore, intravascular sonotherapy may be an effective form of nonionizing energy to reduce in-stent restenosis.
Electron Transfer Dynamics and Surface Coverages of Binary Anthraquinone Monolayers on Mercury Microelectrodes (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectron Transfer Dynamics and Surface Coverages of Binary Anthraquinone Monolayers on Mercury MicroelectrodesRobert J. ForsterCite this: Langmuir 1995, 11, 6, 2247–2255Publication Date (Print):June 1, 1995Publication History Published online1 May 2002Published inissue 1 June 1995https://pubs.acs.org/doi/10.1021/la00006a064https://doi.org/10.1021/la00006a064research-articleACS PublicationsRequest reuse permissionsArticle Views334Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effect of phase errors on field patterns generated by an ultrasound phased-array hyperthermia applicator (1991)

The effect of phase quantization errors and Gaussian distributed random phase errors on field patterns synthesized by a rectangular ultrasound phased array hyperthermia applicator is studied. The parameters defined show that, over the range of four-bit to one-bit quantization, the simulated, patterns degrade with increasing phase errors. However, the overall shape and position of the foci remain unchanged. Simulation results show that, even with one-bit phase quantization, or with a Gaussian distributed random phase error of standard deviation of about 52 degrees , the applicator can still produce useful, if not particularly clean, power deposition patterns. Thus, the power deposition patterns are remarkably stable tolerating quite large phase error levels. This suggests that the power deposition patterns inside the body may be relatively insensitive to phase errors due to tissue inhomogeneities.
Potential for False Positive Identifications from Large Databases through Tandem Mass Spectrometry (2004)

The biomedical research community at large is increasingly employing shotgun proteomics for large-scale identification of proteins from enzymatic digests. Typically, the approach used to identify proteins and peptides from tandem mass spectral data is based on the matching of experimentally generated tandem mass spectra to the theoretical best match from a protein database. Here, we present the potential difficulties of using such an approach without statistical consideration of the false positive rate, especially when large databases, as are encountered in eukaryotes are considered. This is illustrated by searching a dataset generated from a multidimensional separation of a eukaryotic tryptic digest against an in silico generated random protein database, which generated a significant number of positive matches, even when previously suggested score filtering criteria are used.
Fast 3D position measurement with two unsynchronized cameras (2004)

Stereo vision systems have been proposed and used in various fields. They require cameras to synchronize with each other for tracking objects. In this paper, we present a vision system, which uses two normal, unsynchronized cameras for calculating the 3D position of objects. The unsynchronization in shutter timing of cameras is used to process more images per second than a synchronized camera system. 3D positions of an object are measured as a crossing point of lines in 3D through the detected position on the latest frame and estimated 3D position calculated by fast frames. This makes it possible for our system to consist of two unsynchronized cameras and allows for a PC to calculate the 3D position of the moving object at 60 fps. This can be useful for controlling a robot as a fast visual feedback system.
ChemInform Abstract: Heterocyclic Seven‐Membered Ring Compounds. Part 31. The Photochemical Behavior of Monosubstituted 1‐Benzothiepines. (1987)

Abstract Deuteration of (I) and subsequent enolization and alkylation leads to the selectively C‐4 deuterated (III) (98%).
H.264/AVC error resilience tools suitable for 3G mobile video services * (2005)

The emergence of third generation mobile system (3G) makes video transmission in wireless environment possible, and the latest 3GPP/3GPP2 standards require 3G terminals support H.264/AVC. Due to high packet loss rate in wireless envi- ronment, error resilience for 3G terminals is necessary. Moreover, because of the hardware restrictions, 3G mobile terminals support only part of H.264/AVC error resilience tool. This paper analyzes various error resilience tools and their functions, and presents 2 error resilience strategies for 3G mobile streaming video services and mobile conversational services. Performances of the proposed error resilience strategies were tested using off-line common test conditions. Experiments showed that the proposed error resilience strategies can yield reasonably satisfactory results.
Immunization of chickens with live Escherichia coli expressing Eimeria acervulina merozoite recombinant antigen induces partial protection against coccidiosis (1989)

Inoculation of chickens with live Escherichia coli N6405 transformants containing a plasmid which encodes ampicillin resistance and an immunodominant p250 surface antigen of Eimeria acervulina merozoites induced partial protection against challenge with live coccidia. The inoculation with E. coli transformants induced antigen-specific immunoglobulin and cell-mediated immune responses. Challenge with infective oocysts of Eimeria acervulina enhanced both immune parameters, indicating that administration of live E. coli transformants served to prime the immune system for recognition of specific epitopes on the 250-kilodalton protein. Although the mechanism of antigen presentation is unclear, the data suggest that in vivo expression of recombinant merozoite antigen is operative. After administration, no E. coli N6405 transformants could be recovered from intestinal or fecal materials of inoculated chickens, as assessed by enumeration on selective medium. However, ampicillin-resistant E. coli originating from the normal flora and harboring the gene sequences for both antibiotic resistance and Eimeria acervulina merozoite surface protein could be recovered from these chickens. Furthermore, normal-flora E. coli transformants were capable of generating functional beta-lactamase product, as evidenced by their resistance to ampicillin, and immunoreactive E. acervulina merozoite recombinant antigen, as revealed by immunofluorescence staining with p250-specific antiserum.
A Nonlinear Regression Analysis Method for Frame Erasure Concealment in VoIP Networks (2009)

Frame erasure is one of the most difficult problems in voice over IP (VoIP) networks and is a major source of speech quality degradation. In this paper, a frame erasure concealment algorithm based on nonlinear regression analysis is presented to minimize speech quality deterioration in code-excited linear prediction (CELP) based coders. We applied the proposed scheme to the ITU-T G.729 standard and obtained improved perceptual evaluation of speech quality (PESQ) scores compared to the conventional methods.