gebhart/patrepeval huggingface dataset:
Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis. (1990)

By intrasplenic immunization we raised a rat mAb (mAb V1q; IgG2a, kappa) with a potent neutralizing activity against natural mouse TNF (1 microgram/ml mAb V1q/100 U/ml TNF). mAb V1q was used to study the role of endogenous TNF in experimental peritonitis induced by sublethal cecal ligation and puncture. mAb V1q persisted for over 5 days in the serum of mice injected with 100 micrograms of the antibody and, therefore, proved useful for in vivo experiments. As little as 20 micrograms mAb V1q/mouse prevented lethal shock of the animals by 400 micrograms LPS/mouse. In sublethal cecal ligation and puncture i.p. injection of mAb V1q directly and up to 8 h after induction of experimental peritonitis lead to death of the animals within 1 to 3 days. The lethal effect of mAb V1q was compensated by injection of recombinant mouse TNF. Similar mAb V1q effects as in immunocompetent mice were shown in severe combined immune deficiency mice deficient of mature functional B and T cells. Taken together, these data suggest that during the early phase of peritonitis endogenous TNF may stimulate nonlymphoid cells such as granulocytes, macrophages, platelets, and fibroblasts to ingest bacteria and to localize inflammation, respectively. These beneficial effects of TNF may determine survival. Thus, our data may have implications for the therapeutic management of a beginning peritonitis.
Antibodies Catalyze Reactions Otherwise Difficult To Achieve (1993)

RETURN TO ISSUEPREVNewsNEXTAntibodies Catalyze Reactions Otherwise Difficult To Achieve■ Researchers create antibodies to catalyze disfavored reaction and stereo- and regioselective reduction of diketoneRUDY M. BAUMView Author Information C&EN West Coast BureauCite this: Chem. Eng. News 1993, 71, 16, 33–35Publication Date (Print):April 19, 1993Publication History Published online11 November 2010Published inissue 19 April 1993https://pubs.acs.org/doi/10.1021/cen-v071n016.p033https://doi.org/10.1021/cen-v071n016.p033newsACS PublicationsCopyright © 1993 AMERICAN CHEMICAL SOCIETYArticle Views7Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Biopolymers,Chemical reactions,Immunology,Peptides and proteins Get e-Alerts
Red Soils Harbor Diverse Culturable Actinomycetes That Are Promising Sources of Novel Secondary Metabolites (2015)

Red soils, which are widely distributed in tropical and subtropical regions of southern China, are characterized by low organic carbon, high content of iron oxides, and acidity and, hence, are likely to be ideal habitats for acidophilic actinomycetes. However, the diversity and biosynthetic potential of actinomycetes in such habitats are underexplored. Here, a total of 600 actinomycete strains were isolated from red soils collected in Jiangxi Province in southeast China. 16S rRNA gene sequence analysis revealed a high diversity of the isolates, which were distributed into 26 genera, 10 families, and 7 orders within the class Actinobacteria; these taxa contained at least 49 phylotypes that are likely to represent new species within 15 genera. The isolates showed good physiological potentials for biosynthesis and biocontrol. Chemical screening of 107 semirandomly selected isolates spanning 20 genera revealed the presence of at least 193 secondary metabolites from 52 isolates, of which 125 compounds from 39 isolates of 12 genera were putatively novel. Macrolides, polyethers, diketopiperazines, and siderophores accounted for most of the known compounds. The structures of six novel compounds were elucidated, two of which had a unique skeleton and represented characteristic secondary metabolites of a putative novel Streptomyces phylotype. These results demonstrate that red soils are rich reservoirs for diverse culturable actinomycetes, notably members of the families Streptomycetaceae, Pseudonocardiaceae, and Streptosporangiaceae, with the capacity to synthesize novel bioactive compounds.
The Origin of Language (2023)

This book, The Origin of Language: Tracing the Evolution of the Mother Tongue, originally published in 1994 by John Wiley & Sons, was written in a more popular style, accessible to an educated general audience, than the more scholarly and academic tome of a similar title, On the Origin of Languages: Studies in Linguistic Taxonomy, published the same year. In The Origin of Language Ruhlen laid out the principles of linguistic genetic classification, i.e., classifying languages into families according to common origins rather than typological features. Ruhlen showed how simple this can be, especially for languages that have diverged for a few millennia, by juxtaposing short lists of basic (non-cultural) words like eye, fire, and tongue. He also showed that the same methods can be used to postulate older and deeper families, often called “macro-families” or “macrophyla,” by comparing reconstructed forms from lower-level families. Such deeper families (e.g., Nostratic, Dene-Caucasian, Nilo-Saharan, Austric) are generally more controversial than lower-level families, but Ruhlen did not shy from discussing them if he thought the evidence supported them. Ruhlen was also interested in other fields of anthropology, such as archaeology and human genetics, and brought these fields into play.
Glucocorticoid inhibits thrombin-induced expression of platelet-derived growth factor A-chain and heparin-binding epidermal growth factor-like growth factor in human aortic smooth muscle cells. (1993)

Proliferation of smooth muscle cells (SMCs) in atherosclerosis may be modulated by several growth regulatory molecules. At least two mitogens for SMCs, platelet-derived growth factor (PDGF) A-chain and heparin-binding epidermal growth factor-like growth factor (HB-EGF), can be produced by SMCs themselves and may stimulate smooth muscle proliferation in an autocrine or paracrine fashion. We examined the effects of thrombin, which may be generated at the site of vascular injury during atherogenesis, and the potent anti-inflammatory glucocorticoid, dexamethasone (DEX), on the expression of the genes encoding these two growth factors. Since both PDGF A-chain and HB-EGF have affinity for heparin, we also examined the effect of thrombin and DEX on the release of heparin binding mitogenic activity from SMCs. Treatment of SMCs with thrombin resulted in increases both in the level of the PDGF-A and HB-EGF transcripts in the cells, as well as in released heparin-binding growth factor activity. DEX inhibits the thrombin-stimulated release of mitogenic activity in a dose-dependent manner. An enzyme-linked immunoadsorbent assay showed that DEX inhibits both constitutive and thrombin-stimulated release of PDGF-AA. DEX also decreases both constitutive and thrombin-stimulated mRNA levels for PDGF A-chain and HB-EGF and destabilizes the transcripts for both growth factors. A nuclear run-on assay revealed that DEX acts, in addition, to inhibit constitutive and thrombin-stimulated transcription of the PDGF A-chain and HB-EGF genes. Thus, these findings indicate that expression of PDGF A-chain and HB-EGF may be regulated by thrombin and glucocorticoid at the transcription level. Our results are consistent with the involvement of thrombin-induced growth factor expression in neointimal SMC proliferation and suggest the possibility that intimal proliferation may be attenuated by glucocorticoids.
Intron‐mediated enhancement of transgene expression in maize is a nuclear, gene‐dependent process (1997)

In monocots, transgene expression can be stimulated by over two magnitudes by including an intron in the 5' untranslated region (UTR). The underlying mechanism is presently unknown. Inclusion of the salT intron into the 5' UTR of cat and bar genes stimulated expression of the first gene only, indicating that intron-mediated enhancement of expression (IME) is gene-dependent. Stimulation was associated with increased cat RNA levels, which did not result from a reduced cytoplasmic turnover and were not associated with increased translation. This implies that IME acts in the nucleus. Importantly, the cytoplasmic accumulation of spliced cat transcripts, even with IME, is less than that encoded by the intronless bar gene. As the cat and bar genes were flanked by identical regulatory signals, and the transcripts had a similar cytoplasmic stability it may mean that IME rescues rather than stimulates gene expression.
High-performance decoders for regular and irregular repeat-accumulate codes (2005)

This paper investigates high-performance decoder design for regular and irregular repeat-accumulate (RA) codes of large block length. In order to achieve throughputs and bit-error rate performance that are inline with future trends in high-speed communications. high-throughput and low-power decoders of low complexity are needed. To meet such conflicting requirements for long codes, the concept of architecture-aware RA (AARA) code design is proposed. AARA code design decouples the complexity of the decoder from the owe structure by inducing structural regularity features that are amenable to efficient and scalable decoder implementations. Design methods of AARA codes with structured permuters for which an iterative decoding algorithm performs well under message-passing are analogous to those for AA LDPC codes. Algorithmic and architectural optimizations that address the latency, memory overhead, and complexity problems typical of iterative decoders for long RA codes are investigated, and a staggered decoding schedule is introduced. AARA decoders using the proposed schedule have substantial advantage over serial and parallel RA decoders.
Intraoperative augmented reality for minimally invasive liver interventions (2003)

Minimally invasive liver interventions demand a lot of experience due to the limited access to the field of operation. In particular, the correct placement of the trocar and the navigation within the patient's body are hampered. In this work, we present an intraoperative augmented reality system (IARS) that directly projects preoperatively planned information and structures extracted from CT data, onto the real laparoscopic video images. Our system consists of a preoperative planning tool for liver surgery and an intraoperative real time visualization component. The planning software takes into account the individual anatomy of the intrahepatic vessels and determines the vascular territories. Methods for fast segmentation of the liver parenchyma, of the intrahepatic vessels and of liver lesions are provided. In addition, very efficient algorithms for skeletonization and vascular analysis allowing the approximation of patient-individual liver vascular territories are included. The intraoperative visualization is based on a standard graphics adapter for hardware accelerated high performance direct volume rendering. The preoperative CT data is rigidly registered to the patient position by the use of fiducials that are attached to the patient's body, and anatomical landmarks in combination with an electro-magnetic navigation system. Our system was evaluated in vivo during a minimally invasive intervention simulation in a swine under anesthesia.
Redundant logarithmic arithmetic (1990)

A number system that offers advantages in some situations over conventional floating point and sign/logarithmic number systems is described. Redundant logarithmic arithmetic, like conventional logarithmic arithmetic, relies on table lookups to make the arithmetic unit simpler than an equivalent floating point unit. The cost of 32 bit subtraction in a redundant logarithmic number system is lower than previously published logarithmic subtraction methods. The total memory requirement for a 29-bit redundant logarithmic unit is 16 K words compared to 22 K words by the best previously published conventional sign logarithm unit, assuming similar addition techniques are employed. A redundant logarithmic number system can be implemented with online arithmetic, which would be impractical for a conventional sign logarithm number system. The disadvantages of redundant arithmetic are typical of redundant number systems. First, the redundancy doubles the storage requirements for data values. Second, the representation can become ill-conditioned, especially as a result of iterated multiplications. Third, division and square root operations are more difficult to implement in redundant logarithmic arithmetic.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Multi-Cell MIMO Cooperative Networks: A New Look at Interference (2010)

This paper presents an overview of the theory and currently known techniques for multi-cell MIMO (multiple input multiple output) cooperation in wireless networks. In dense networks where interference emerges as the key capacity-limiting factor, multi-cell cooperation can dramatically improve the system performance. Remarkably, such techniques literally exploit inter-cell interference by allowing the user data to be jointly processed by several interfering base stations, thus mimicking the benefits of a large virtual MIMO array. Multi-cell MIMO cooperation concepts are examined from different perspectives, including an examination of the fundamental information-theoretic limits, a review of the coding and signal processing algorithmic developments, and, going beyond that, consideration of very practical issues related to scalability and system-level integration. A few promising and quite fundamental research avenues are also suggested.
Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin α 4 β 1 (CD49d/CD29) (1996)

Anti-alpha 4 integrin mAb coprecipitated CD81 (TAPA-1), a 25-kDa cell surface protein, from various alpha 4 beta1 -positive hemopoietic cell lines, including Molt4, Jurkat, Ramos, and alpha 4-transfected K562 (KX4C4) cells. In reciprocal experiments, the integrin alpha 4 beta 1 (VLA4, CD49d/CD29) could be reprecipitated from CD81 immunoprecipitates. Anti-alpha 4 integrin mAb also coprecipitated CD81 from the alpha 4 beta 7-positive B cell line RPMI 8866. In contrast, no CD81 was identified in alpha 2 beta 1, alpha 5 beta 1, or alpha L beta 2 immunoprecipitates. Abs to other members of the transmembrane-4 superfamily, including CD53, CD63, and CD82, also coprecipitated alpha 4 beta 1. As shown by confocal microscopy, CD81 and CD82 colocalized with alpha 4 beta 1 in cell surface clusters. The cytoplasmic domain of the alpha 4 integrin was not necessary for alpha 4 beta 1/CD81 association, nor was the association influenced by divalent cations, EDTA, integrin-activating mAb, or alpha 4 subunit cleavage. Notably, two independent alpha 4 adhesion-deficient mutants (D346E and D408E) were deficient in their ability to associate with CD81. Thus, CD81 and other transmembrane-4 superfamily members may participate in functionally relevant interactions with alpha 4 beta 1 and other integrins.
Integrin αvβ3‐targeted cancer therapy (2008)

Anti-angiogenesis is a promising strategy for the treatment of cancer. Integrins, consisting of two noncovalently bound transmembrane alpha and beta subunits, are an important molecular family involved in tumor angiogenesis. The blockade of integrin signaling has been demonstrated to be efficient to inhibit tumor growth, angiogenesis, and metastasis. Among all the integrins, alpha(v)beta(3) seems to be the most important one during tumor angiogenesis. The inhibition of integrin alpha(v)beta(3) signaling with antibodies, peptides, peptidomimetics, and other antagonists has great potential in the treatment of cancer. In addition, integrin alpha(v)beta(3) is highly expressed on activated endothelial cells, new-born vessels as well as some tumor cells, but is not present in resting endothelial cells and most normal organ systems, making it a suitable target for anti-angiogenic therapy. In this article we will review the role of integrin alpha(v)beta(3) in angiogenesis, present recent progress in the use of integrin alpha(v)beta(3) antagonists and integrin-targeted delivery systems as potential cancer therapeutics, and discuss future perspectives.
Multilevel Optimum Design of Structures with Fiber-Composite Stiffened-Panel Components (1982)

The multilevel approach to minimum weight structural design is extended to wing box structures with fiber-composite stiffened-panel components. Strength, deflection, and panel buckling constraints are treated at the system level with equivalent-thickness-type design variables. Local buckling and panel buckling constraints are guarded against at the component level, employing detailed component dimensions as design variables. A key feature of the method is selection of change in stiffness as the component level objective function to be minimized. Numerical results are given for wing box structures with sandwich and hat-stiffened fiber-composite panels.
Illuminant estimation for object recognition (2002)

Abstract Comparing colour histograms of images has been shown to be a powerful technique for discriminating among large sets of images. However, these histograms depend not only on the properties of imaged objects but also on the illumination under which the objects are captured. If this illumination dependence is not accounted for prior to constructing the colour histograms, colour‐based image indexing will fail when illumination changes. This failure can be addressed by correcting the RGBs in an image to corresponding RGBs representing the same scene but under a standard reference illuminant prior to constructing the histograms. To perform this correction of RGBs, it is necessary to have a measurement or, more commonly, an estimate of the illumination in the original scene. Many authors have proposed illuminant estimation (or colour constancy) algorithms to obtain such an estimate. Unfortunately, the results of colour histogram matching experiments under varying illumination conditions have shown that existing estimation algorithms do not provide a sufficiently good estimate of the scene illuminant to enable this approach to work. In this article we report on the results of our repetition of those experiments, but this time using a new illuminant estimation algorithm—the so‐called color by correlation approach, which has been shown to afford significantly better performance than previous algorithms. The results of this new experiment show that when this new algorithm is used to preprocess images, a significant improvement in colour histogram matching performance is achieved. Indeed, performance is close to the theoretically optimal level of performance, that is, close to that which can be obtained using actual measurements of the scene illumination. © 2002 Wiley Periodicals, Inc. Col Res Appl, 27, 260–270, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/col.10064
Theory of Complex Spectra. II (1942)

The spectra of two-electron configurations in ($\mathrm{jj}$) and ($\mathrm{jl}$) coupling and of the configurations ${d}^{n}$, ${f}^{3}$, ${d}^{2}p$, and ${d}^{8}p$ in ($\mathrm{LS}$) coupling are calculated with tensor operators. The agreement with the odd terms of Ti II and Ni II is satisfactory. It is also proved that $\frac{{G}^{k}}{(2k+1)}$ is a positive decreasing function of $k$.
n‐3 (omega‐3) polyunsaturated fatty acids prevent acute atrial electrophysiological remodeling (2006)

We conclude that acute n-3 PUFA treatment prevents acute atrial electrophysiological remodeling during high rate activity, which may minimize the self-perpetuation of AF.
Shear‐Controlled Single‐Step Mouse Embryonic Stem Cell Expansion and Embryoid Body–Based Differentiation (2005)

To facilitate the exploitation of embryonic stem cells (ESCs) and ESC-derived cells, scale-up of cell production and optimization of culture conditions are necessary. Conventional ESC culture methods are impractical for large-scale cell production and lack robust microenvironmental control. We developed two stirred-suspension culture systems for the propagation of undifferentiated ESCs--microcarrier and aggregate cultures--and compared them with tissue-culture flask and Petri dish controls. ESCs cultured on glass microcarriers had population doubling times (approximately 14-17 hours) comparable to tissue-culture flask controls. ESC growth could be elicited in shear-controlled stirred-suspension culture, with population doubling times ranging between 24 and 39 hours at 100 rpm impeller speed. Upon removal of leukemia inhibitory factor, the size-controlled ESC aggregates developed into embryoid bodies (EBs) capable of multilineage differentiation. A comprehensive analysis of ESC developmental potential, including flow cytometry for Oct-4, SSEA-1, and E-cadherinprotein expression, reverse transcription-polymerase chain reaction for Flk-1, HNF3-beta, MHC, and Sox-1 gene expression, and EB differentiation analysis, demonstrated that the suspension-cultured ESCs retained the developmental potential of the starting cell population. Analysis of E-cadherin-/- and E-cadherin+/- cells using both systems provided insight into the mechanisms behind the role of cell aggregation control, which is fundamental to these observations. These cell-culture tools should prove useful for both the production of ESCs and ESC-derived cells and for investigations into adhesion, survival, and differentiation phenomena during ESC propagation and differentiation.