gebhart/patrepeval huggingface dataset:
Retargeting of Viruses to Generate Oncolytic Agents (2011)

Oncolytic virus therapy is based on the ability of viruses to effectively infect and kill tumor cells without destroying the normal tissues. While some viruses seem to have a natural preference for tumor cells, most viruses require the modification of their tropism to specifically enter and replicate in such cells. This review aims to describe the transductional targeting strategies currently employed to specifically redirect viruses towards surface receptors on tumor cells. Three major strategies can be distinguished; they involve (i) the incorporation of new targeting specificity into a viral surface protein, (ii) the incorporation of a scaffold into a viral surface protein to allow the attachment of targeting moieties, and (iii) the use of bispecific adapters to mediate targeting of a virus to a specified moiety on a tumor cell. Of each strategy key features, advantages and limitations are discussed and examples are given. Because of their potential to cause sustained, multiround infection-a desirable characteristic for eradicating tumors-particular attention is given to viruses engineered to become self-targeted by the genomic expression of a bispecific adapter protein.
Defining the Diverse Cell Populations Contributing to Lignification in Arabidopsis Stems (2017)

Many land plants evolved tall and sturdy growth habits due to specialized cells with thick lignified cell walls: tracheary elements that function in water transport and fibers that function in structural support. The objective of this study was to define how and when diverse cell populations contribute lignin precursors, monolignols, to secondary cell walls during lignification of the Arabidopsis (<i>Arabidopsis thaliana</i>) inflorescence stem. Previous work demonstrated that, when lignin biosynthesis is suppressed in fiber and tracheary element cells with thickened walls, fibers become lignin-depleted while vascular bundles still lignify, suggesting that nonlignifying neighboring xylem cells are contributing to lignification. In this work, we dissect the contributions of different cell types, specifically xylary parenchyma and fiber cells, to lignification of the stem using cell-type-specific promoters to either knock down an essential monolignol biosynthetic gene or to introduce novel monolignol conjugates. Analysis of either reductions in lignin in knockdown lines, or the addition of novel monolignol conjugates, directly identifies the xylary parenchyma and fiber cell populations that contribute to the stem lignification and the developmental timing at which each contribution is most important.
Efficacy of MRI and Mammography for Breast-Cancer Screening in Women with a Familial or Genetic Predisposition (2004)

MRI appears to be more sensitive than mammography in detecting tumors in women with an inherited susceptibility to breast cancer.
Evidence from transgenic mice that glucokinase is rate limiting for glucose utilization in the liver (1996)

To study the role of glucokinase (GK) in the control of glucose metabolism in the liver, transgenic mice were generated in which GK was overexpressed under control of the P-enolpyruvate carboxykinase gene promoter. Whereas the expression of the GK gene in starved control mice was blocked, this promoter was able to direct the expression of the enzyme to the liver of starved transgenic mice. Furthermore, starved transgenic mice showed levels of GK activity fourfold higher than those of starved control and similar to those of fed control. This activation of GK led to an increase in the intracellular concentration of glucose 6-phosphate, which was also related to an induction of glycogen accumulation. In addition, L-pyruvate kinase (L-PK) activity increased in transgenic mice, which when starved showed similar levels of activity to control fed mice. The induction of L-PK caused an increase in the hepatic lactate concentration. Furthermore, hepatocytes in primary culture from transgenic mice incubated with 20 mM glucose produced levels of lactate threefold higher than controls, but no difference was noted when the hepatocytes from control and transgenic mice were incubated with 2 mM glucose. These results demonstrated in vivo that the activation of GK is a rate-limiting step in the induction of glycolysis and glycogen synthesis. These changes in liver glucose metabolism led to a marked reduction in blood glucose (30%) and insulin (40%) concentrations. Furthermore, transgenic mice showed lower levels of blood glucose after an intraperitoneal glucose tolerance test, indicating that GK overexpression caused an increase in blood glucose disposal by the liver. All these findings show the key role of liver GK in the control of whole-body glucose homeostasis.-Ferre, T., Riu, E., Bosch, F., Valera, A. Evidence from transgenic mice that glucokinase is rate limiting for glucose utilization in the liver.
Function of an N‐Heterocyclic Carbene Ligand Based on Concept of Chiral Mimetic. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
From epoxomicin to carfilzomib: chemistry, biology, and medical outcomes (2013)

The initial enthusiasm following the discovery of a pharmacologically active natural product is often fleeting due to the poor prospects for its ultimate clinical application. Despite this, the ever-changing landscape of modern biology has a constant need for molecular probes that can aid in our understanding of biological processes. After its initial discovery by Bristol-Myers Squibb as a microbial anti-tumor natural product, epoxomicin was deemed unfit for development due to its peptide structure and potentially labile epoxyketone pharmacophore. Despite its drawbacks, epoxomicin's pharmacophore was found to provide unprecedented selectivity for the proteasome. Epoxomicin also served as a scaffold for the generation of a synthetic tetrapeptide epoxyketone with improved activity, YU-101, which became the parent lead compound of carfilzomib (Kyprolis™), the recently approved therapeutic agent for multiple myeloma. In this era of rational drug design and high-throughput screening, the prospects for turning an active natural product into an approved therapy are often slim. However, by understanding the journey that began with the discovery of epoxomicin and ended with the successful use of carfilzomib in the clinic, we may find new insights into the keys for success in natural product-based drug discovery.
Cell‐Surface display of heterologous proteins: From high‐throughput screening to environmental applications (2002)

A variety of expression systems for the display of either short peptides or fully folded proteins on E.coli and, to a lesser extent, on Gram-positive bacteria have been developed. The expression of proteins on the surface of microbial cells has proved extremely important for numerous applications ranging from combinatorial library screening and protein engineering, to whole cell biocatalysts and adsorbants for bioremediation purposes.
Stochastic Games (1953)

Understanding the biological basis of social anxiety disorder (SAD), one of the most disabling of the anxiety disorders, will allow for novel treatment strategies to be developed. Here, we show that gut microbiota may be such a target. Mice ...Social anxiety disorder (SAD) is a crippling psychiatric disorder characterized by intense fear or anxiety in social situations and their avoidance. However, the underlying biology of SAD is unclear and better treatments are needed. Recently, the gut ...
A Highly Sensitive and Quantitative Test Platform for Detection of NSCLC EGFR Mutations in Urine and Plasma (2016)

DNA derived from NSCLC tumors can be detected with high sensitivity in urine and plasma, enabling diagnostic detection and monitoring of therapeutic response from these noninvasive "liquid biopsy" samples.
Retention of an idiotypic determinant in a human B-cell lymphoma undergoing immunoglobulin variable-region mutation. (1987)

Tumor cells from a patient with B-cell lymphoma were fused with a mouse myeloma cell line. A set of heterohybridomas was thus derived, each of which represented a separate clonal derivative from the tumor cell population. The immunoglobulins secreted by these cell lines reacted variably with a panel of anti-idiotypic antibodies, indicating that the tumor was heterogeneous; however, one antibody, 4D6, reacted strongly with the product of all the heterohybridomas. cDNA for the immunoglobulin heavy chain variable-region genes expressed in these heterohybridomas was cloned and sequenced. Comparison of these sequences indicated that the cells expressing them were clonally related but that they had undergone considerable mutation. Despite mutation, the cells in this tumor population continued to express a functional immunoglobulin molecule and to retain, over a span of 3 years, the idiotypic determinant defined by the 4D6 monoclonal antibody. Thus a selective force existed within the host to retain tumor cells bearing an immunoglobulin molecule with a particular idiotypic structure.
Evidence for presence of an internal thiolester bond in third component of human complement. (1980)

Treatment of the third component of human complement (C3) with methylamine results in a loss of hemolytic function and the appearance of a thiol group. Studies with [14C]methylamine have indicatd a stoichiometric and covalent reaction with the native protein. Hydrazine-inactivated C3 and C3b prepared with bovine trypsin were unreactive with [14C]-methylamine. Alkylation experiments with [1-14C]iodoacetamide have further established a 1:1 correspondence between methylamine incorporation and expression of the reactive thiol. Autoradiographic analyses of [14C]methylamine-treated C3 and methylamine-inactivated [1-14C]carboxyamidomethylated C3 after NaDodSO4/polyacrylamide gel electrophoresis have shown a specific incorporation of each radiolabel into the alpha polypeptide chain. [14C]Methylamine-treated C3 was immobilized on Sepharose 4B by reaction of the protein thiol with a mixed disulfide. Digestion with bovine trypsin in 4 M urea released 96% of the bound absorbance (at 280 nm) units; the radiolabel remained associated with the Sepharose beads. Peptide material labeled with 14C was eluted with dithiothreitol, carboxymethylated with [3H]iodoacetic acid, and chromatographed on Sephadex G-75. On Edman degradation S-[3H]carboxymethylcysteine was released at step 9 and gamma-glutamyl[14C]-methylamide was released at step 12. We interpret these data to indicate the presence of an internal thiolester bond in native C3. In addition, evidence is presented for an identical reactive site in alpha 2-macroglobulin.
Mobile peer-to-peer content sharing application (2006)

The demo presents a novel architecture of Mobile Peer-to-Peer (MP2P) content sharing services in cellular networks. The proposed solution is based on Session Initiation Protocol (SIP) that is part of the IP Multimedia Subsystem (IMS) in third-generation cellular networks. The concept has been implemented in C++ on the Symbian Series 60 platform. The conducted trial showed the feasibility of proposed approach and opens new room for development of scalable services in the mobile environment.
Spin-flip transitions between Zeeman sublevels in semiconductor quantum dots (2001)

We have studied spin-flip transitions between Zeeman sublevels in GaAs electron quantum dots. Several different mechanisms which originate from spin-orbit coupling are shown to be responsible for such processes. It is shown that spin-lattice relaxation for the electron localized in a quantum dot is much less effective than for the free electron. The spin-flip rates due to several other mechanisms not related to the spin-orbit interaction are also estimated.
Dissociation Constants of the Conjugate Acids of Substituted Benzyl Phenyl Ketones and of Alkyl-substituted Benzophenones (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDissociation Constants of the Conjugate Acids of Substituted Benzyl Phenyl Ketones and of Alkyl-substituted BenzophenonesA. Fischer, B. A. Grigor, J. Packer, and J. VaughanCite this: J. Am. Chem. Soc. 1961, 83, 20, 4208–4210Publication Date (Print):October 1, 1961Publication History Published online1 May 2002Published inissue 1 October 1961https://pubs.acs.org/doi/10.1021/ja01481a026https://doi.org/10.1021/ja01481a026research-articleACS PublicationsRequest reuse permissionsArticle Views126Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Biomimetic synthesis of dibromophakellin (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiomimetic synthesis of dibromophakellinLouise H. Foley and George BuechiCite this: J. Am. Chem. Soc. 1982, 104, 6, 1776–1777Publication Date (Print):March 1, 1982Publication History Published online1 May 2002Published inissue 1 March 1982https://pubs.acs.org/doi/10.1021/ja00370a072https://doi.org/10.1021/ja00370a072research-articleACS PublicationsRequest reuse permissionsArticle Views758Altmetric-Citations111LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Halocin H6, a Bacteriocin from Haloferax gibbonsii (1989)

Haloferax gibbonsii strain Ma2.39 produces an inhibitor substance designated halocin H6. This halocin was isolated from culture supernatants of the producer strain. It appeared to be a non-inducible bacteriocin with a bactericidal mode of cation and typical ‘single-hit’ kinetics. The protein was purified by a combination of hydroxylapatite affinity chromatography, gel filtration and HPLC. Halocin H6 is a protein of M r 32000; it is heat resistant, non-salt-dependent (it retains its activity after exposure to distilled water) and sensitive to pronase but not to trypsin.
Gene‐Encoded Peptide Antibiotics and the Concept of Innate Immunity: An Update Review (1998)

Antibacterial peptides were first considered rather species-specific. However, the perspective began to change in 1987-89. Five years later there were two symposium volumes and several reviews on gene-encoded peptide antibiotics which covered the known peptides irrespective of origin. The field is rapidly growing and a first update was published in this Journal in 1996. At that time a database was made with about 100 entries; now it has over 400, with some redundancy. Recently a methodological handbook was published and there are many specialized reviews covering only defensins or insect immunity. In the last 2 years, the larger perspective of innate immunity and the role of gene-mediated peptide antibiotics have evolved in ways which justify a new update. Today insects and plants are known to have similar overall design of their defensins while insects and mammals have very similar control mechanisms. The signal pathways are beginning to appear and the future perspective may involve additional changes.
Epitope Location on Tissue Factor Determines the Anticoagulant Potency of Monoclonal Anti-tissue Factor Antibodies (2000)

Summary Tissue factor (TF), the cellular cofactor for the serine protease factor VIIa (F. VIIa), triggers blood coagulation and is involved in the pathogenesis of various thrombosis-related disorders. Therefore, agents which specifically target tissue factor, such as monoclonal antibodies, may provide promising new antithrombotic therapy. We mapped the epitopes of several anti-TF antibodies using a panel of soluble TF mutants. They bound to three distinct TF regions. The epitope of the 7G11 antibody included Phe50 and overlapped with a TF-F. VIIa light chain contact area. The common epitope of the antibodies 6B4 and HTF1 included residues Tyr94 and Phe76 both of which make critical contacts to the catalytic domain of F. VIIa. The antibodies D3 and 5G6 had a common epitope outside the TF-F. VIIa contact region. It included residues Lys165, Lys166, Asn199, Arg200 and Lys201 and thus overlapped with the substrate interaction region of tissue factor. The antibodies 5G6 and D3 were potent anticoagulants when infused to flowing human blood in an ex-vivo thrombosis model. Plasma fibrinopeptide A levels and fibrin deposition were completely inhibited. In contrast, 6B4 was a weak inhibitor in this ex-vivo thrombosis model, and HTF1 displayed no inhibition at all. These disparate activities were also reflected in TF-dependent F. X activation assays performed with human plasma. The potency differences could neither be explained by the determined binding affinities nor by the on-rates of antibodies. Therefore, the results suggest that antibody binding epitope and hence the particular mechanism of inhibition, is the main determinative factor of anticoagulant potency of anti-TF antibodies.
Co‐opting biology to deliver drugs (2014)

The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is protein-based delivery systems--albumin, transferrin, and fusion to the Fc domain of antibodies--that have a long-circulation half-life and are designed to transport different molecules. The second class is lipid-based delivery systems-lipoproteins and exosomes-that are naturally occurring circulating lipid particles. The third class is cell-based delivery systems--erythrocytes, macrophages, and platelets--that have evolved, for reasons central to their function, to exhibit a long life-time in the body. The last class is small molecule-based delivery systems that include folic acid. This article reviews the biology of these systems, their application in drug delivery, and the promises and limitations of these endogenous systems for drug delivery.
Granulierung hydrophober Wirkstoffe im Planetwalzenextruder (2003)

For hydrophobic actives the binder granulation is a widespread method to achieve sufficient tabletting properties. An intensive contact between binder and active during granulation is a prerequisite for a successful granulation due to the mostly hydrophilic behavior of the common used binders. This present article describes the possibility of granulations with very low binder concentrations in the planetary roller extruder, leading to a good tabletability of the granules. In the same way the tablets show outstanding disintegration properties and dissolution profiles while the active concentration in the final dosage form is up to 92 %.
Artificial Muscle Technology: Physical Principles and Naval Prospects (2004)

The increasing understanding of the advantages offered by fish and insect-like locomotion is creating a demand for muscle-like materials capable of mimicking nature's mechanisms. Actuator materials that employ voltage, field, light, or temperature driven dimensional changes to produce forces and displacements are suggesting new approaches to propulsion and maneuverability. Fundamental properties of these new materials are presented, and examples of potential undersea applications are examined in order to assist those involved in device design and in actuator research to evaluate the current status and the developing potential of these artificial muscle technologies. Technologies described are based on newly explored materials developed over the past decade, and also on older materials whose properties are not widely known. The materials are dielectric elastomers, ferroelectric polymers, liquid crystal elastomers, thermal and ferroelectric shape memory alloys, ionic polymer/metal composites, conducting polymers, and carbon nanotubes. Relative merits and challenges associated with the artificial muscle technologies are elucidated in two case studies. A summary table provides a quick guide to all technologies that are discussed.
Synthesis and Characterization of All Renewable Resources Based Branched Polyester: Poly(2,5-furandicarboxylic acid-co-glycerol) (2013)

Condensation of all renewable resources based monomers 2,5-furandicarboxylic acid and glycerol in the presence of 2 mol% Sb 2 O 3 catalyst at 210°C gives a branched polyester resin in 70% yield. This resin was characterized by elemental analysis, 1 H, 13 C NMR, FT-IR, and TGA-DTG.
Jones calculus analysis of single-mode fiber Sagnac reflector (1988)

It is postulated that stray birefringence degrades the performance of the single-mode fiber Sagnac reflector. An expression for the birefringence dependence of reflector performance is derived using the Jones calculus and is used as the basis for a theoretical model of the spectral response of a real Sagnac reflector. The experimental behavior of the reflector with (1) different levels of stress applied to the fiber loop and (2) different lengths of highly birefringent fiber spliced into the loop are compared with the model. This comparison confirms the initial postulate.
C-reactive protein influences shear stress-dependent platelet adhesion in patients with familiar hypercholesterolemia and coronary artery disease undergoing LDL apheresis (2006)

C-reactive protein influences shear stress-dependent platelet adhesion in patients with familiar hypercholesterolemia and coronary artery disease undergoing LDL apheresis -