gebhart/patrepeval huggingface dataset:
Effect of picotamide on the clinical progression of peripheral vascular disease. A double-blind placebo-controlled study. The ADEP Group. (1993)

The results of this study indicate that picotamide reduces cardiovascular complications in PVD patients. The apparently low effect of this drug in reducing major events suggests that further studies be made with picotamide in PVD patients who are at high risk of cardiovascular complications so as to further assess its clinical efficacy.
Tetracyclines Inhibit Protein Glycation in Experimental Diabetes (1998)

Glycation of proteins, which is accelerated in the diabetic state, has been implicated in many of the long-term complications of diabetes. This process can be inhibited by members of the tetracycline family of compounds. This novel finding is supported by studies conducted on drug (streptozotocin)induced Type I and genetic (ZDF/Gmi-fa/fa) Type II diabetic rats. These animals were orally gavaged daily with 5 mg of doxycycline and a variety of non-antimicrobial chemically modified tetracycline derivatives for time periods of 3 weeks to 11 months, while control untreated diabetic and nondiabetic animals were gavaged with vehicle alone (2% CMC). Blood and tissue samples were collected and analyzed for glucose and glycated proteins. None of the treatments had any effect on the severity of hyperglycemia or the intracellular glycation of hemoglobin of either Type I or II diabetic animals. However, the tetracycline analogues did affect the extracellular glycation of several proteins such as those found in the serum as well as skin collagen. In the Type II (ZDF) animals, initial mortality (3-5 months) was seen only in the doxycycline-treated animals, associated with infection by tetracycline-resistant micro-organisms, which was eventually surpassed by mortality rates in the untreated diabetics (6-9 months). CMT treatment not only decreased mortality but also increased longevity in the Type II diabetic animals, most likely by preventing the development of a number of long-term complications of uncontrolled diabetes, including glycation of proteins, that eventually lead to the demise of untreated diabetic animals.
Functional Dissection of Eyes absent Reveals New Modes of Regulation within the Retinal Determination Gene Network (2003)

The retinal determination (RD) gene network encodes a group of transcription factors and cofactors necessary for eye development. Transcriptional and posttranslational regulation of RD family members is achieved through interactions within the network and with extracellular signaling pathways, including epidermal growth factor receptor/RAS/mitogen-activated protein kinase (MAPK), transforming growth factor beta/DPP, Wingless, Hedgehog, and Notch. Here we present the results of structure-function analyses that reveal novel aspects of Eyes absent (EYA) function and regulation. We find that the conserved C-terminal EYA domain negatively regulates EYA transactivation potential, and that GROUCHO-SINE OCULIS (SO) interactions provide another mechanism for negative regulation of EYA-SO target genes. We have mapped the transactivation potential of EYA to an internal proline-, serine-, and threonine-rich region that includes the EYA domain 2 (ED2) and two MAPK phosphorylation consensus sites and demonstrate that activation of the RAS/MAPK pathway potentiates transcriptional output of EYA and the EYA-SO complex in certain contexts. Drosophila S2 cell two-hybrid assays were used to describe a novel homotypic interaction that is mediated by EYA's N terminus. Our data suggest that EYA requires homo- and heterotypic interactions and RAS/MAPK signaling responsiveness to ensure context-appropriate RD gene network activity.
Cache coherence protocols: evaluation using a multiprocessor simulation model (1986)

Using simulation, we examine the efficiency of several distributed, hardware-based solutions to the cache coherence problem in shared-bus multiprocessors. For each of the approaches, the associated protocol is outlined. The simulation model is described, and results from that model are presented. The magnitude of the potential performance difference between the various approaches indicates that the choice of coherence solution is very important in the design of an efficient shared-bus multiprocessor, since it may limit the number of processors in the system.
Mesenchymal Stem Cells: Potential Precursors for Tumor Stroma and Targeted-Delivery Vehicles for Anticancer Agents (2004)

Injected MSC-IFN-beta cells suppressed the growth of pulmonary metastases, presumably through the local production of IFN-beta in the tumor microenvironment. MSC may be an effective platform for the targeted delivery of therapeutic proteins to cancer sites.
Immunosuppressive properties of human amniotic membrane for mixed lymphocyte reaction (2002)

The combination of allograft limbal transplantation (ALT) and amniotic membrane transplantation (AMT) has been applied in the treatment of severe ocular surface diseases. The beneficial effect of this combination has been thought to result from possible immunosuppressive ability of amniotic membrane (AM). However, the mechanisms of any such ability remain unknown. In this study, we investigated whether human AM has the ability to suppress allo-reactive T cell responses in vitro. For mixed lymphocyte reaction (MLR), lymphocytes isolated from lymph nodes of C57BL/6 mice (Mls1b, Vbeta6+) were cultured with irradiated splenocytes from DBA/2 mice (Mls1a, Vbeta6-) with or without human AM. For carboxyfluorescein diacetate succinimidyl ester (CFSE) experiments, responder lymph node cells were labelled with a stable intracellular fluorescent dye and cultured with irradiated stimulator cells. The ratio of responder Vbeta6+ T cells was then determined by FACS analysis, and the division profiles of responder Vbeta6+ T cells were analysed by CFSE content. Furthermore, Th1 and Th2 cytokine synthesis by allo-reactive T cells in MLR culture supernatants was determined by enzyme-linked immunosorbent assay (ELISA). Addition of AM to the MLR culture resulted in the significant inhibition of thymidine incorporation compared with control culture lacking AM. The population of responder CD4+Vbeta6+ T cells was significantly reduced in the AM-treated culture in comparison to control. CFSE analysis revealed less division and lower proliferation of responder CD4+Vbeta6+ T cells in cultures with AM than without. In addition, allo-rective T cell synthesis of both Th1 (IL-2 and IFNgamma) and Th2 (IL-6 and IL-10) type cytokine was significantly decreased in the presence of AM. These results indicate that human AM has the ability to suppress allo-reactive T cells in vitro. This inhibitory effect likely contributes to the success of the ALT-AMT combination.
Effects of anti-erbB-2 (HER-2/neu) recombinant oncotoxin AR209 on human non-small cell lung carcinoma grown orthotopically in athymic nude mice. (1996)

The recombinant oncotoxin AR209 [e23(Fv)PE38KDEL; formerly OLX-209] was developed to treat neoplasia that expresses the c-erbB-2 (HER-2/neu) protein product p185(erbB-2). The AR209 compound contains a single-chain antibody domain specific for p185(erbB-2), coupled with a portion of the Pseudomonas exotoxin. The drug has been shown to be effective in inhibiting cells that overexpress erbB-2 due to gene amplification and in cells that do not contain amplified erbB-2 but express slightly higher levels of the protein than normal cells do. To test the efficacy of AR209 on human lung tumors, athymic nude mice were inoculated intrathoracically with a cell line derived from a poorly differentiated lung adenocarcinoma. This cell line, termed 201T, expresses moderately elevated levels of p185(erbB-2) 7.6-fold over normal bronchial epithelium. Mice treated with i.v. injections of AR209 for 5 weeks after orthotopic tumor implantation had smaller tumors and in 20% of cases showed no evidence of disease. The data from this study indicate that AR209 may be an effective treatment for patients with non-small cell lung cancers that express p185(erbB-2).
Analysis of oligodendroglial differentiation using cDNA arrays (2000)

We used cDNA arrays to investigate molecular aspects of the differentiation of an immortalized line of oligodendroglial progenitors, and of immunopan-purified primary cultures of oligodendroglial progenitors, to immature oligodendroglia. Developmental regulation of the proteolipid and 2-hydroxyacylsphingosine 1-galactosyltransferase genes was tighter in the primary than in the immortalized cells. Our data suggest that increased expression of genes encoding the following proteins are involved in oligodendroglial differentiation: Fyn, Erk, p85, G-alpha-12 guanine nucleotide binding, and transducin beta-2 signal transduction molecules; glial maturation factor; the proteasomal subunits C8 and C3; the proteasomal targeting molecule polyubiquitin; the cell cycle regulatory proteins Set, protein phosphatase 2A, and nuclear tyrosine phosphatase (PRL-1); and the high-affinity glutamate cotransporter EAAC-1.
Apoptosis and DNA fragmentation precede TNF‐induced cytolysis in U937 cells (1992)

The hypothesis that activation of apoptosis and DNA fragmentation is involved in TNF-mediated cytolysis of U937 tumor cells was investigated. Morphological, biochemical, and kinetic criteria established that TNF activates apoptosis as opposed to necrosis. Within 2-3 h of exposure to TNF, U937 underwent the morphological alterations characteristic of apoptosis. This was accompanied by cleavage of DNA into multiples of nucleosome size fragments. Both of these events occurred 1-2 h prior to cell death as defined by trypan blue exclusion or 51Cr release. DNA fragmentation was not a non-specific result of cell death since U937 cells lysed under hypotonic conditions did not release DNA fragments. The percentage of cells undergoing apoptosis depended on the concentration of TNF and was augmented by the addition of cycloheximide. A TNF-resistant variant derived from U937 did not undergo apoptosis in response to TNF, even in the presence of cycloheximide. Furthermore, TNF could still activate NFkB in this variant, suggesting that this pathway is not involved in TNF-mediated cytotoxicity. Two agents known to inhibit TNF-mediated cytotoxicity, ZnSO4 and 3-aminobenzamide, were shown to inhibit TNF-induced apoptosis. Taken altogether, these data support the hypothesis that activation of apoptosis is at least one essential step in the TNF lytic pathway in the U937 model system.
Synthesis of Conformationally Restricted Amino Acids and Peptides Employing Olefin Metathesis (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Conformationally Restricted Amino Acids and Peptides Employing Olefin MetathesisScott J. Miller and Robert H. GrubbsCite this: J. Am. Chem. Soc. 1995, 117, 21, 5855–5856Publication Date (Print):May 1, 1995Publication History Published online1 May 2002Published inissue 1 May 1995https://pubs.acs.org/doi/10.1021/ja00126a027https://doi.org/10.1021/ja00126a027research-articleACS PublicationsRequest reuse permissionsArticle Views1429Altmetric-Citations170LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
A Deletion Mutation Placing the Galactokinase Gene of Escherichia coli under Control of the Biotin Promoter (1974)

A deletion has been isolated which fuses galK and presumably galT to the promoter and operator of the bioA operon. The deletion has endpoints in bioA and galE, and leaves the operator-proximal end of bioA intact and the regulatory sites themselves functional. In bacterial strains which carry the deletion, the expression of galK is regulated by biotin, although due to the inefficiency of the bioA promoter even the fully derepressed level of galactokinase is low. Electron micrographic examination of the mutation shows that it is a simple deletion, uncomplicated by other chromosomal abnormalities.
A perylene diimide (PDI)-based small molecule with tetrahedral configuration as a non-fullerene acceptor for organic solar cells (2015)

In this paper, a new perylene diimide (PDI)-based acceptor<bold>Me-PDI4</bold>with tetrahedral configuration (or 3D) has been synthesized and characterized.
When Limited Partners Default (2002)

As equity markets continue to struggle, the prospect of having limited partners default on their capital contributions becomes more of a possibility. Many venture capitalists are quite concerned about certain portions of their partnership agreements, such as the flexibility allowed the general partner to make investments, but far less concerned about the penalty provisions in caseof default. This article outlines possible courses of action and discusses which ones make the most sense in different circumstances.
Increasing data rate over wireless channels (2000)

Space-time coding (STC) is a new coding/signal processing framework for wireless communication systems with multiple transmit and multiple receive antennas. This new framework has the potential of dramatically improve the capacity and data rates. In addition, this framework presents the best trade-off between spectral efficiency and power consumption. ST codes (designed so far) come in two different types. ST trellis codes offer the maximum possible diversity gain and a coding gain without any sacrifice in the transmission bandwidth. The decoding of these codes, however, would require the use of a vector form of the Viterbi decoder. Space-time block codes (STBCs) offer a much simpler may of obtaining transmit diversity without any sacrifice in bandwidth and without requiring huge decoding complexity. In fact, the structure of the STBCs is such that it allows for very simple signal processing (linear combining) for encoding/decoding, differential encoding/detection, and interference cancellation. This new signal processing framework offered by ST codes can be used to enhance the data rate and/or capacity in various wireless applications. That is the reason many of these STC ideas have already found their way to some of the current third-generation wireless systems standards.
Reactive Cysteines of the Yeast Plasma-Membrane H+-ATPase (PMA1) (1997)

We have taken advantage of cysteine mutants described previously (Petrov, V. V., and Slayman, C. W. (1995) J. Biol. Chem. 270, 28535-28540) to map the sites at which N-ethylmaleimide (NEM) reacts with the plasma-membrane H+ATPase (PMA)1 of Saccharomyces cerevisiae. When membrane vesicles containing the ATPase were incubated with NEM, six of nine mutants with single cysteine substitutions showed sensitivity similar to the wild-type enzyme. By contrast, C221A and C532A were inactivated more slowly than the wild-type control, and the C221, 532A double mutant was completely resistant, indicating that Cys-221 and Cys-532 are NEM-reactive residues. In the presence of 10 mM MgADP, the wild-type ATPase was partially protected against NEM; parallel experiments with the C221A and C532A mutants showed that the protection occurred at Cys-532, located in or near the nucleotide-binding site. Unexpectedly, the inactivation of the C409A ATPase was approximately 4-fold more rapid than in the case of the wild-type enzyme. Experiments with double mutants made it clear that this resulted from an acidic shift in pKa and a consequent acceleration of the reaction rate at Cys-532. One simple interpretation is that substitution of Cys-409 leads to a local conformational change within the central hydrophilic domain. Consistent with this idea, the reaction of fluorescein 5'-isothiocyanate at Lys-474 was also stimulated approximately 3. 5-fold by the C409A mutation. Taken together, the results of this study provide new information about the reactivity of individual Cys residues within the ATPase and pave the way to tag specific sites for structural and functional studies of the enzyme.
Restoration of wide band signal from telephone speech using linear prediction error processing (2002)

The paper describes a system that can enhance the quality of speech signals that are severely band limited during regular telephone speech transmission. The author has already proposed a spectrum widening method that utilizes aliasing in sampling rate conversion and digital filtering for spectrum shaping. The paper proposes a new method using linear prediction (LP) residual error processing and parameter (coefficients) mapping using linear extrapolation that offers improved performance in terms of the spectrum distortion characteristics. Real time hardware implementation procedures are discussed. The proposed method can effectively enhance speech quality.
Selection of Human Antibodies from Phage Display Libraries (2004)

Phage display can be used to generate human antibodies to virtually any antigen. This requires a number of crucial steps that are common to all molecular diversity technologies: the creation of diversity, coupling of phenotype (antibody protein) to genotype (antibody gene), selection, amplification, and analysis (reviewed in ref. 1–3). Antibodies with affinities comparable to those obtained using traditional hybridoma technology can be selected from either large naïve antibody libraries or from immune phage antibody libraries. The affinity of initial isolates can be further increased, to levels unobtainable in the immune system, by using the selected antibodies as the basis for construction and selection of libraries in which the antibody gene is diversified (see Chapters 19 and 20 ). This approach is one of the few methods for generating fully human antibodies, especially to evolutionarily conserved or self proteins (4). Although in theory there are many different choices to be made when constructing a phage antibody library, most published work has followed a tried and tested formula. In particular, filamentous phage-based phagemid vectors have been used almost exclusively, and the phage minor coat protein pIII has been the display coat protein most frequently used (2,5). Libraries have been made using both the single-chain Fv (scFv) (6–10) and Fab format (11,12).
Toward Paperlike Displays (2001)

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Extended single-mode photonic crystal fiber lasers (2006)

We report on an ytterbium-doped photonic crystal fiber with a core diameter of 60 microm and mode-field-area of ~2000 microm(2) of the emitted fundamental mode. Together with the short absorption length of 0.5 m this fiber possesses a record low nonlinearity which makes this fiber predestinated for the amplification of short laser pulses to very high peak powers. In a first continuous-wave experiment a power of 320 W has been extracted corresponding to 550 W per meter. To our knowledge this represents the highest power per unit length ever reported for fiber lasers. Furthermore, the robust single-transverse-mode propagation in a passive 100 microm core fiber with a similar design reveals the potential of extended large-mode-area photonic crystal fibers.
The Synergistic In Vitro and In Vivo Antitumor Effect of Combination Therapy with Salinomycin and 5-Fluorouracil against Hepatocellular Carcinoma (2014)

Hepatocellular carcinoma (HCC) is one of the few cancers in which a continuous increase in incidence has been observed over several years. Drug resistance is a major problem in the treatment of HCC. In the present study, we used salinomycin (Sal) and 5-fluorouracil (5-FU) combination therapy on HCC cell lines Huh7, LM3 and SMMC-7721 and nude mice subcutaneously tumor model to study whether Sal could increase the sensitivity of hepatoma cells to the traditional chemotherapeutic agent such as 5-FU. The combination of Sal and 5-FU resulted in a synergistic antitumor effect against liver tumors both in vitro and in vivo. Sal reversed the 5-FU-induced increase in CD133(+) EPCAM(+) cells, epithelial-mesenchymal transition and activation of the Wnt/β-catenin signaling pathway. The combination of Sal and 5-FU may provide us with a new approach to reverse drug resistant for the treatment of patients with HCC.
Granulocyte Colony‐Stimulating Factor Mobilizes More Dendritic Cell Subsets Than Granulocyte‐Macrophage Colony‐Stimulating Factor with No Polarization of Dendritic Cell Subsets in Normal Donors (2006)

Dendritic cells (DCs) are effective antigen‐presenting cells. We hypothesized that increasing the DC populations in donor lymphocyte infusions (DLIs) may augment the graft versus malignancy effect, particularly if granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) mobilization resulted in increased precursor dendritic cell (pDC) 1 cells. Mature DCs, pDC1 cells, pDC2 cells, and CD34+ cells from the same donor were compared after granulocyte colony‐stimulating factor (G‐CSF) mobilized peripheral blood stem cell collections and GM‐CSF mobilized DLI collections. Mobilization with G‐CSF resulted in up to a 10‐fold larger number of CD34+ cells per kg and a 3–5‐fold larger number of mature DCs, pDC1 cells, and pDC2 cells within the same donor compared with GM‐CSF. The ratio of pDC1 to pDC2 in each donor remained constant with either cytokine. In this small sample of normal donors, it appears that G‐CSF mobilizes more CD34+ cells, mature DCs, pDC1 cells, and pDC2 cells within the same donor than does GM‐CSF, with no significant polarization by G‐CSF or GM‐CSF for either pDC1 or pDC2 cells.
Characterization of the O-Glycosylation Sites in the Chorionic Gonadotropin β Subunit in Vivo Using Site-directed Mutagenesis and Gene Transfer (1996)

Human chorionic gonadotropin (CG) is a member of a family of glycoprotein hormones which are heterodimers containing two nonidentical subunits: a common alpha and a hormone-specific beta subunit. One of the distinguishing features of the CGbeta subunit is the presence of four serine acceptors clustered within the last 25 amino acids. We previously demonstrated that this carboxyl-terminal region is important for maintaining its biologic half-life, and when the sequence was genetically fused to either the common alpha or follitropin beta subunits, O-glycosylation was observed. Because this carboxyl-terminal sequence is located at the end of the subunit, we considered this region a convenient in vivo model for studying O-linked glycosylation in domains containing multiple serine recognition sites. A CGbeta gene was engineered in which the N-linked sites were inactivated to eliminate background from those carbohydrate groups. Using this construct, we made a series of truncation and amino acid substitutions of acceptor serines, and these mutants were transfected into Chinese hamster ovary cells. O-Glycosylation was determined by [3H]glucosamine incorporation and glycanase sensitivity of the products on SDS-polyacrylamide gels. We show that the O-linked sites comprise independent repetitive regions in which each acceptor serine has a recognition signal bounded by the next carboxy acceptor serine within four to five amino acids. It is also apparent that recognition of one site is not dependent on the glycosylation of another acceptor. Amino acid mutations in the acceptor regions demonstrated the importance of proline as a necessary feature for O-linked recognition in the CGbeta sequence.