gebhart/patrepeval huggingface dataset:
Relationship of cAMP and calcium messenger systems in prostaglandin-stimulated UMR-106 cells. (1988)

The effect of prostaglandins (PG) on free cytosolic calcium concentrations [( Ca2+]i) and cAMP levels was studied in the osteosarcoma cell line UMR-106. PGF2 alpha and PGE2, but not 6-keto-PGF1 alpha, induced an increase in [Ca2+]i which was mainly due to Ca2+ release from intracellular stores. The EC50 for PGF2 alpha was approximately 7 nM, whereas that for PGE2 was approximately 1.8 microM. Maximal doses of PGF2 alpha increased [Ca2+]i to higher levels than PGE2. Both active PGs also stimulated phosphatidylinositol turnover in UMR-106 cells. The effects of the two PGs were independent of each other and appear to involve separate receptors for each PG. PGE2 was a very potent stimulator of cAMP production and increased cAMP by approximately 80-fold with an EC50 of 0.073 microM. PGF2 alpha was a very poor stimulator of cAMP production; 25 microM PGF2 alpha increased cAMP by 5-fold. The increase in cellular cAMP levels activated a plasma membrane Ca2+ channel which resulted in a secondary, slow increase in [Ca2+]i. High concentrations of both PGs (10-50 microM) inhibited this channel independent of their effect on cAMP levels. Pretreatment of the cells with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate inhibited the PG-mediated increase in phosphatidylinositol turnover and the increase in [Ca2+]i. However, pretreatment with 12-O-tetradecanoyl-13-acetate had no effect on the PGE2-mediated increase in cAMP. The latter finding, together with the dose responses for PGE2-mediated increases in [Ca2+]i and cAMP levels, suggests the presence of two subclasses of PGE2 receptors: one coupled to adenylate cyclase and the other to phospholipase C. With respect to osteoblast function, the cAMP signaling system is antiproliferative, whereas the Ca2+ messenger system, although having no proliferative effect by itself, tempers cAMP's antiproliferative effect.
Tumor Necrosis Factor-α Activates the Sphingomyelin Signal Transduction Pathway in a Cell-Free System (1992)

The mechanism of tumor necrosis factor (TNF)-alpha signaling is unknown. TNF-alpha signaling may involve sphingomyelin hydrolysis to ceramide by a sphingomyelinase and stimulation of a ceramide-activated protein kinase. In a cell-free system, TNF-alpha induced a rapid reduction in membrane sphingomyelin content and a quantitative elevation in ceramide concentrations. Ceramide-activated protein kinase activity also increased. Kinase activation was mimicked by addition of sphingomyelinase but not by phospholipases A2, C, or D. Reconstitution of this cascade in a cell-free system demonstrates tight coupling to the receptor, suggesting this is a signal transduction pathway for TNF-alpha.
Studies on Herpes Simplex Virus III. The Effects of Roentgen Radiation, Cortisone and Gastric Mucin Upon the Infectivity of Herpes Simplex Virus for Laboratory Mice (1955)

Journal Article Studies on Herpes Simplex Virus III. The Effects of Roentgen Radiation, Cortisone and Gastric Mucin Upon the Infectivity of Herpes Simplex Virus for Laboratory Mice Get access Jerome T. Syverton, Jerome T. Syverton From the Departments of Bacteriology and Immunology and of Radiology, University of Minnesota, Minneapolis 14, Minnesota Search for other works by this author on: Oxford Academic PubMed Google Scholar Garabed A. Garabedian, Garabed A. Garabedian From the Departments of Bacteriology and Immunology and of Radiology, University of Minnesota, Minneapolis 14, Minnesota Search for other works by this author on: Oxford Academic PubMed Google Scholar Jack Friedman Jack Friedman From the Departments of Bacteriology and Immunology and of Radiology, University of Minnesota, Minneapolis 14, Minnesota Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 96, Issue 1, January 1955, Pages 9–13, https://doi.org/10.1093/infdis/96.1.9 Published: 01 January 1955 Article history Received: 19 October 1953 Published: 01 January 1955
Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur. (1995)

Mice that carry the lethal yellow (Ay) or viable yellow (Avy) mutation, two dominant mutations of the agouti (a) gene in mouse chromosome 2, exhibit a phenotype that includes yellow fur, marked obesity, a form of type II diabetes associated with insulin resistance, and an increased susceptibility to tumor development. Molecular analyses of these and several other dominant "obese yellow" a-locus mutations suggested that ectopic expression of the normal agouti protein gives rise to this complex pleiotropic phenotype. We have now tested this hypothesis directly by generating transgenic mice that ectopically express an agouti cDNA clone encoding the normal agouti protein in all tissues examined. Transgenic mice of both sexes have yellow fur, become obese, and develop hyperinsulinemia. In addition, male transgenic mice develop hyperglycemia by 12-20 weeks of age. These results demonstrate conclusively that the ectopic agouti expression is responsible for most, if not all, of the phenotypic traits of the dominant, obese yellow mutants.
Gas-phase proton-transfer reactions between alkoxide anions (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGas-phase proton-transfer reactions between alkoxide anionsJames A. Dodd, Susan Baer, Christopher R. Moylan, and John I. BraumanCite this: J. Am. Chem. Soc. 1991, 113, 16, 5942–5949Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/ja00016a004https://doi.org/10.1021/ja00016a004research-articleACS PublicationsRequest reuse permissionsArticle Views96Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Sparse Direction of Arrival Estimation Using Co-Prime Arrays with Off-Grid Targets (2013)

In this letter, we consider the problem of direction of arrival estimation using sparsity enforced reconstruction methods. Co-prime arrays with <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$M + N$</tex></formula> sensors are utilized to increase the degrees of the freedom from <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex Notation="TeX">$O(M + N)$</tex></formula> to <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex Notation="TeX">$O(MN)$</tex></formula> . The key to the success of sparse-based direction of arrival estimation is that every target must fall on the predefined grid. Off-grid targets can highly jeopardize the reconstruction performance. In this letter, we use joint sparsity reconstruction methods to explore the underlying structure between the sparse signal and the gird mismatch. Two types of sparse reconstruction methods, the greedy method and the convex relaxation method, are considered. By implementing numerical experiments, we demonstrate that our proposed methods can fully utilize the virtual aperture created by co-prime arrays and also outperform the previously proposed MUSIC method with spatial smoothing.
Low Circulating Levels of Insulin‐Like Growth Factors and Testosterone in Chronically Institutionalized Elderly Men (1993)

Compared with healthy young men, most healthy old men have low serum IGF-I, TT, and FT levels. The geriatric hyposomatomedinemia and hypogonadism are more severe in institutionalized old men. In the latter group, both endocrine deficiencies are usually of central origin, but their occurrences are not significantly associated. Healthy old men usually have a low level of IGF-I compared with healthy young men, but a similar level of IGF-II; institutionalized old men are usually low in both values.
Fluidization of nanopowders: a review (2012)

Nanoparticles (NPs) are applied in a wide range of processes, and their use continues to increase. Fluidization is one of the best techniques available to disperse and process NPs. NPs cannot be fluidized individually; they fluidize as very porous agglomerates. The objective of this article is to review the developments in nanopowder fluidization. Often, it is needed to apply an assistance method, such as vibration or microjets, to obtain proper fluidization. These methods can greatly improve the fluidization characteristics, strongly increase the bed expansion, and lead to a better mixing of the bed material. Several approaches have been applied to model the behavior of fluidized nanopowders. The average size of fluidized NP agglomerates can be estimated using a force balance or by a modified Richardson and Zaki equation. Some first attempts have been made to apply computational fluid dynamics. Fluidization can also be used to provide individual NPs with a thin coating of another material and to mix two different species of nanopowder. The application of nanopowder fluidization in practice is still limited, but a wide range of potential applications is foreseen. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11051-012-0737-4) contains supplementary material, which is available to authorized users.
Correlation of expressions of GFAP, NT-3, Trk and NCAM with neurotropic molecular mechanism and clinical factors in adenoid cystic carcinoma of salivary gland (2009)

OBJECTIVE:To observe the expressions of GFAP, NT-3, Trk and NCAM in order to investigate the mechanism of perineural invasion (PI), and the correlation of them with clinical factors in ACC. METHODS: The expressions of GFAP, NT-3, Trk and NCAM were examined in 94 cases of ACC by immunohistochemical means, and the differences of their expressions were analyzed between(among) PI/NPI(NO PI) groups and clinical groups with different factors. RESULTS: There were differences of excessive expressions of GFAP, NT-3, Trk, and low expressions of NCAM between(among) the PI/NPI groups and pathological hypotype groups (P0.05); and the excessive expressions of NT-3 and Trk were different between the recurrence/no recurrence groups (P0.05); the excessive expression of NT-3 was different between the margin infiltration/no infiltration groups (P0.05). Multiplicity further indicated: there was a positive correlation of expressions of GFAP, NT-3 and Trk with NI(β values were 2.097, 1.871 and 3.194, respectively; P0.05), and the negative correlation of expression of GFAP with pathological hypotypes in ACC(β=-1.216, P=0.021). In addition, the expression of NCAM in nerve tissue was higher than that in ACC cells. CONCLUSION: The excessive expressions of GFAP, NT-3 and Trk possibly participate in PI of ACC, and the low expression of NCAM possibly indirectly promotes the process of PI.
Cancer Chemoprevention Agent Development Strategies for Genistein (1995)

Cancer chemoprevention refers to the reduction of cancer incidence by administration of agents or drugs that inhibit, reverse or retard the cancer process. Genistein has demonstrated a wide variety of biological activities that make it a good candidate for a chemopreventive agent. Many agents, such as genistein, are currently being tested with the goal of developing safe and effective chemopreventive drugs for human use. Genistein was investigated as a potential chemopreventive agent in an azoxymethane-induced colon carcinogenesis model. Genistein was tested for its ability to inhibit aberrant colon crypts in the colon of F344 rats that had been treated with azoxymethane. Genistein was administered in the diet from 1 wk before the carcinogen to 4 wk after the first carcinogen dose for a total of 5 wk. At both doses, 75 and 150 mg/kg, the mean number of foci per colon was significantly reduced. Further development of this agent includes demonstration of the preventive efficacy in an in vivo tumorigenesis model, followed by preclinical pharmacology and toxicology testing. Phase 1, 2 and 3 clinical chemoprevention trials would be then performed to determine pharmacokinetics, safe doses, and effectiveness for New Drug Approval.
Catalytic Hydrogenation Activity and Electronic Structure Determination of Bis(arylimidazol-2-ylidene)pyridine Cobalt Alkyl and Hydride Complexes (2013)

The bis(arylimidazol-2-ylidene)pyridine cobalt methyl complex, ((iPr)CNC)CoCH3, was evaluated for the catalytic hydrogenation of alkenes. At 22 °C and 4 atm of H2 pressure, ((iPr)CNC)CoCH3 is an effective precatalyst for the hydrogenation of sterically hindered, unactivated alkenes such as trans-methylstilbene, 1-methyl-1-cyclohexene, and 2,3-dimethyl-2-butene, representing one of the most active cobalt hydrogenation catalysts reported to date. Preparation of the cobalt hydride complex, ((iPr)CNC)CoH, was accomplished by hydrogenation of ((iPr)CNC)CoCH3. Over the course of 3 h at 22 °C, migration of the metal hydride to the 4-position of the pyridine ring yielded (4-H2-(iPr)CNC)CoN2. Similar alkyl migration was observed upon treatment of ((iPr)CNC)CoH with 1,1-diphenylethylene. This reactivity raised the question as to whether this class of chelate is redox-active, engaging in radical chemistry with the cobalt center. A combination of structural, spectroscopic, and computational studies was conducted and provided definitive evidence for bis(arylimidazol-2-ylidene)pyridine radicals in reduced cobalt chemistry. Spin density calculations established that the radicals were localized on the pyridine ring, accounting for the observed reactivity, and suggest that a wide family of pyridine-based pincers may also be redox-active.
Structure-Activity Relationship within a Series of Degradation Products of Tautomycin (1996)

Tautomycin, a protein serine/threonine phosphatase inhibitor, was chemically degraded, and five derivatives were investigated for their biological activities. None of them exerted any inhibitory effects on the activity of protein phosphatase types 1 and 2A. However, one derivative, named TM2a, induced a significant morphological change (bleb-formation) of human myeloid leukemia K562 cells. TM2b, the trimethyl ester of TM2, did not induce bleb-formation. Thus, the maleic anhydride structure played an important role in the biological activity. The biological properties of TM2a toward K562 cells resembled those of a phorbol ester, rather than of tautomycin. The phorbol ester-induced bleb formation was abrogated by a non-specific inhibitor of protein kinases, staurosporine, and by an inhibitor of protein kinase C (PKC), H-7, but TM2a-induced bleb formation was abrogated only by staurosporine. Enhanced phosphorylation of the two proteins was observed after their exposure to TM2a. This suggest that the effect was not due to any inhibition of protein phosphatase 1 or 2A, but rather to the activation of an unidentified kinase, possibly of the PKC family, or to inhibition of a protein phosphatase other than type 1 or 2A.
Mechanism and fidelity of HIV reverse transcriptase. (1992)

We have examined the RNA-dependent and DNA-dependent polymerase and ribonuclease H catalytic activities of human immunodeficiency virus reverse transcriptase using rapid transient kinetic methods with defined synthetic 25/45-mer DNA/RNA and DNA/DNA primer/templates. The Kd value for interaction of the enzyme with duplex DNA was 4.7 nM, and the value for RNA/DNA heteroduplex was of similar magnitude. A pre-steady state burst of nucleoside triphosphate incorporation was observed for both DNA and RNA templates. Analysis of the dATP concentration dependence of the burst rate provided Kd values for dATP of 4 and 14 microM and maximum rates of single nucleotide incorporation, kpol, of 33 and 74 s-1, for DNA and RNA templates, respectively. Subsequent turnovers were limited by the rate of dissociation of the primer/template from the enzyme at rates of 0.18 and 0.06 s-1 for duplex DNA and RNA/DNA heteroduplex, respectively. Analysis of rates of DNA polymerization and RNA cleavage using the RNA template revealed that the two activities are independent of one another. The polymerization rate (4-70 s-1) was dependent on dATP concentration, whereas the RNA cleavage occurred at a constant rate of 10 s-1 over the 100-fold dATP concentration range (2-200 microM). Examination of the RNA cleavage products resulting from a single turnover indicates that the polymerase and ribonuclease domains of the enzyme are separated by a distance corresponding to 19 bases of RNA/DNA heteroduplex, consistent with the recently published crystal structure (Kohlstaedt, L. A., Wang, J., Friedman, J., Rice, P. A., and Steitz, T. A. (1992) Science 256, 1783-1790). Analysis of the kinetics of processive synthesis suggested that the initial binding of dNTP leads to a faster rate of dissociation of DNA from the enzyme. Further investigation supported a two-step dNTP binding mechanism with the formation of an initial E.DNA.dNTP complex followed by a more stable E'.DNA.dNTP complex. The Kd values for incorporation of incorrect nucleoside triphosphates opposite a DNA template thymidine were 1010 microM for dGTP, 1240 microM for dCTP, and 840 microM for dTTP. The corresponding maximum kpol rates were 4.8 s-1 for dGTP, 0.52 s-1 for dCTP, and 0.41 s-1 for dTTP. These values provide fidelity estimates of 1740 for discrimination against dGTP, 19,700 for dCTP, and 16,900 for dTTP misincorporations at this site.
IL-4 and IL-10 Are Both Required for the Induction of Oral Tolerance (1999)

Protection from the development of experimental autoimmune uveitis (EAU) can be induced by feeding mice interphotoreceptor retinoid binding protein before uveitogenic challenge with the same protein. Two different regimens are equally effective in inducing protective tolerance, although they seem to do so through different mechanisms: one involving regulatory cytokines (IL-4, IL-10, and TGF-beta), and the other with minimal involvement of cytokines. Here we studied the importance of IL-4 and IL-10 for the development of oral tolerance using mice genetically engineered to lack either one or both of these cytokines. In these animals we were able to protect against EAU only through the regimen inducing cytokine-independent tolerance. When these animals were fed a regimen that in the wild-type animal is thought to predominantly induce regulatory cells and is associated with cytokine secretion, they were not protected from EAU. Interestingly, both regimens were associated with reduced IL-2 production and proliferation in response to interphotoreceptor retinoid binding protein. These findings indicate that both IL-4 and IL-10 are required for induction of protective oral tolerance dependent on regulatory cytokines, and that one cytokine cannot substitute for the other in this process. These data also underscore the fact that oral tolerance, manifested as suppression of proliferation and IL-2 production, is not synonymous with protection from disease.
Active document layout synthesis (2005)

Document layout analysis has been researched for many years. However, there is little work on the reverse of document layout analysis: document layout synthesis, whose goal is to generate logically correct and aesthetically appealing layout given the text/image contents and the flexible layout template. This paper introduces a new automatic document layout synthesis method, which can actively pursue the optimal text block height-width tradeoff simultaneously with the block position adjustment. The key idea is to use a cluster of linear functions to approximate the nonlinear height-width curve. Then two-pass simplex algorithm is used to solve the layout synthesis problem. This method has a wide range of applications, such as variable data printing (VDP), automatic table formatting, and automating document layout ground truth generation.
Training Optimization for Gauss-Markov Rayleigh Fading Channels (2007)

In this paper, pilot-assisted transmission over Gauss-Markov Rayleigh fading channels is considered. A simple scenario, where a single pilot signal is transmitted every T symbols and T - 1 data symbols are transmitted in between the pilots, is studied. First, it is assumed that binary phase-shift keying (BPSK) modulation is employed at the transmitter. With this assumption, the training period, and data and training power allocation are jointly optimized by maximizing an achievable rate expression. Achievable rates and energy-per-bit requirements are computed using the optimal training parameters. Secondly, a capacity lower bound is obtained by considering the error in the estimate as another source of additive Gaussian noise, and the training parameters are optimized by maximizing this lower bound.
Surgery for heart failure (2007)

he failing heart creates a huge health care problem. It is common and becomes more so with age. Recognising our aging population, it is understandable that the prevalence of heart failure is increasing 1 in the UK and is the most common diagnostic related group at hospital discharge. It is the primary or secondary diagnosis in about 1% of the population and consumes a similar proportion of the country's gross domestic product every year. So how do we recognise this monster? The 2005 American College of Cardiology/American Heart Association (ACC/AHA) guideline update for the diagnosis and management of chronic heart failure in the adult 2 defines heart failure as: ''a complex clinical syndrome that can result from any structural or functional cardiac disorder that impairs the ability of the ventricle to fill with or eject blood''.
Impact of mitochondrial β-oxidation in fatty acid-mediated inhibition of glioma cell proliferation (2003)

Tetradecylthioacetic acid (TTA), which cannot be beta-oxidized, exerts growth-limiting properties in glioma cells. In order to investigate the importance of modulated lipid metabolism and alterations in mitochondrial properties in this cell death process, we incubated glioma cells both with TTA and the oxidizable fatty acid palmitic acid (PA), in the presence of L-carnitine and the carnitine palmitoyltransferase inhibitors etomoxir and aminocarnitine. L-carnitine partly abolished the PA-mediated growth reduction of glioma cells, whereas etomoxir and aminocarnitine enhanced the antiproliferative effect of PA. The production of acid-soluble products increased and the incorporation of PA into glycerolipids decreased after L-carnitine supplementation. L-carnitine was found to enhance the antiproliferative effect of TTA, but did not affect the incorporation of TTA into glycerolipids, or ceramide. PDMP, sphingosine 1-phosphate, desipramine, fumonisin B(1), and L-cycloserine were able not to rescue the glioma cells from PA and TTA-induced growth inhibition, suggesting that increased ceramide production is not important in the growth reduction. TTA-mediated growth inhibition was accompanied with an increased uptake of PA and increased incorporation of PA into triacylglycerol (TG). Our data suggest that mitochondrial functions are involved in fatty acid-mediated growth inhibition. Whether there is a causal relationship between TG accumulation and the apoptotic process remains to be determined.