gebhart/patrepeval huggingface dataset:
Lanadelumab to treat hereditary angioedema (2019)

Lanadelumab is a human monoclonal antibody against plasma kallikrein indicated for prevention of attacks of hereditary angioedema (HAE). HAE is caused by SERPING1 gene mutations resulting in decreased or dysfunctional plasma protease C1 inhibitor (C1-INH) leading to a loss of inhibition of plasma kallikrein activity with subsequent cleavage of high-molecular weight kininogen and release of bradykinin. There is a clear need for a non-plasma-derived, safe, effective and convenient prophylaxis of HAE attacks to reduce patients' daily burden of disease and disability. The percentage of patients who were attack-free for the last 16 weeks of a controlled study was 77% in the group receiving 300 mg lanadelumab every 2 weeks, compared with 3% with placebo. The most common side effects were mild injection-site reactions. Lanadelumab has the potential to change the approach from on-demand treatment to prophylaxis in HAE. Future studies will have to confirm long-term safety and efficacy of prophylactic long-term inhibition of plasma kallikrein.
A high voltage stimulator for small electrode electrocutaneous stimulation (2002)

A high voltage stimulator has been designed to allow transcutaneous stimulation of tactile fibers of the fingertip. The output stage is based upon an improved Howland current pump topology, modified to allow high load impedances and small currents. The current output accuracy is better than 15% for 1 to 10 mA for 30 /spl mu/sec pulses. The rise time for square pulses is less than 2 /spl mu/sec. Common mode latch-up power supply problems are discussed.
Unit completion for a computer-aided translation typing system (2000)

This work is in the context of TRANSTYPE, a system that observes its user as he or she types a translation and repeatedly suggests completions for the text already entered. The user may either accept, modify, or ignore these suggestions. We describe the design, implementation, and performance of a prototype which suggests completions of units of texts that are longer than one word.
Shock Ignition: A New Approach to High Gain Inertial Confinement Fusion on the National Ignition Facility (2009)

Shock ignition, an alternative concept for igniting thermonuclear fuel, is explored as a new approach to high gain, inertial confinement fusion targets for the National Ignition Facility (NIF). Results indicate thermonuclear yields of approximately 120-250 MJ may be possible with laser drive energies of 1-1.6 MJ, while gains of approximately 50 may still be achievable at only approximately 0.2 MJ drive energy. The scaling of NIF energy gain with laser energy is found to be G approximately 126E (MJ);{0.510}. This offers the potential for high-gain targets that may lead to smaller, more economic fusion power reactors and a cheaper fusion energy development path.
Identification of a second interferon-regulated murine Mx gene. (1988)

The murine influenza virus resistance locus Mx consists of more than one interferon-responsive transcription unit. We isolated clones of the transcripts of a second Mx gene (Mx2) which is closely linked to the well-characterized resistance gene and determined their sequences. Mx2 mRNA is more than 90% identical to Mx1 mRNA in the region corresponding to the amino termini of the encoded proteins. Mx2 mRNAs of mouse strains BALB/c and CBA have open reading frames apparently interrupted by mutations. Interferon-treated cells from strain A2G and other influenza virus-resistant strains failed to express detectable amounts of Mx2 transcripts.
High efficiency beamline for synchrotron radiation lithography (1991)

A highly efficient beamline for synchrotron radiation (SR) lithography has been developed for our compact storage ring (Super-ALIS). Two toroidal mirrors are employed to increase the x-ray intensity on the wafer and to vertically expand the exposure area. Astigmatism is intentionally introduced in these mirrors to increase their converging and collimating abilities. The first mirror converges SR beams with a divergence angle of 2°. The second mirror, which is controlled by a microcomputer, collimates the beam and oscillates to produce an exposure area of 25×25 mm2. An improved vacuum evaporation technique is used to coat the mirror surfaces with platinum, which increases the reflectivity to 50% at a wavelength of 8.34 Å. The resulting x-ray intensity on the wafer is 5 mW/cm2/100 mA. A uniform x-ray intensity distribution can be obtained by adjusting the scan speed of the second mirror and using an x-ray compensation filter. The resulting nonuniformity is improved to be less than ±4%. A reliable x-ray extraction system that consists of two Be windows and a SiN window permits exposure in an atmospheric environment.
Glutamine targeting inhibits systemic metastasis in the VM‐M3 murine tumor model (2010)

Metastatic cancer is a major cause of morbidity and mortality. Current therapeutic options consist of chemotherapy, radiation or targeted therapies. However, these therapies are often toxic, effective over a small range of cancer types or result in drug resistance. Therefore, a more global, less toxic strategy for the management of metastatic cancer is required. Although most cancers display increased glucose metabolism, glutamine is also a major energy substrate for many cancers. We evaluated the antimetastatic potential of 6-diazo-5-oxo-L-norleucine (DON), a glutamine analog, using the new VM mouse model of systemic metastasis. We found that primary tumor growth was ∼20-fold less in DON-treated mice than in untreated control mice. We also found that DON treatment inhibited metastasis to liver, lung and kidney as detected by bioluminescence imaging and histology. Our findings provide proof of concept that metabolic therapies targeting glutamine metabolism can manage systemic metastatic cancer.
Synthesis and anti-microbial activity of some pyrimidine derivatives (2003)

4-Aryl-5-carboethoxy-6-methyl-1,2,3,4-tetrahydropyrimidin-2-ones have been synthesized from easily available starting materials. The carboethoxy group at the C 5 -position of the pyrimidine ring is converted to corresponding hydrazide which in turn is condensed with cyclising agents such as aromatic aldehydes, CS 2 etc. to give fused heterocycles. The fused heterocycles are then subjected to phenacylation to give N 3 -phenacylpyrimido-heterocycles in excellent yield. In a slightly modified way, uracil derivatives are condensed with ethyl bromoacetate to give N 3 -β-ethoxycarbonyl derivatives. The hydrazide derivatives of these N 3 -β-ethoxycarbonyl derivatives subsequently react with 1,2-diketones to give corresponding pyrimido pyridazine derivatives.
A Gene Expression Profile of Alzheimer's Disease (2001)

Postmortem analysis of brains of patients with Alzheimer's disease (AD) has led to diverse theories about the causes of the pathology, suggesting that this complex disease involves multiple physiological changes. In an effort to better understand the variety and integration of these changes, we generated a gene expression profile for AD brain. Comparing affected and unaffected brain regions in nine controls and six AD cases, we showed that 118 of the 7050 sequences on a broadly representative cDNA microarray were differentially expressed in the amygdala and cingulate cortex, two regions affected early in the disease. The identity of these genes suggests the most prominent upregulated physiological correlates of pathology involve chronic inflammation, cell adhesion, cell proliferation, and protein synthesis (31 upregulated genes). Conversely, downregulated correlates of pathology involve signal transduction, energy metabolism, stress response, synaptic vesicle synthesis and function, calcium binding, and cytoskeleton (87 downregulated genes). The results support several separate theories of the causes of AD pathology, as well as add to the list of genes associated with AD. In addition, approximately 10 genes of unknown function were found to correlate with the pathology.
Emerging roles for bone morphogenetic proteins in central nervous system glial biology (2004)

Bone morphogenetic proteins, members of the TGFbeta superfamily have been implicated in a variety of roles in the developing and mature nervous system. These divergent functions are a reflection of the closely defined spatial and temporal expression of BMPs in the CNS, and the potential interactions of the BMP signaling pathway with the STAT and MAP kinase pathways. In this review we discuss the roles of BMPs in early patterning of the CNS, determination of neural cell fate, and regulation of oligodendrocyte maturation during CNS development. Additional functions for members of the TGFbeta superfamily in CNS injury responses are emerging suggesting these molecules represent useful targets for manipulating neural responses to CNS insults.
Aspartyl beta-hydroxylase: in vitro hydroxylation of a synthetic peptide based on the structure of the first growth factor-like domain of human factor IX. (1989)

beta-Hydroxylation of aspartic acid is a post-translational modification that occurs in several vitamin K-dependent coagulation proteins. By use of a synthetic substrate comprised of the first epidermal growth factor-like domain in human factor IX and either mouse L-cell extracts or rat liver microsomes as the source of enzyme, in vitro aspartyl beta-hydroxylation was accomplished. Aspartyl beta-hydroxylase appears to require the same cofactors as known alpha-ketoglutarate-dependent dioxygenases. The hydroxylation reaction proceeds with the same stereospecificity and occurs only at the aspartate corresponding to the position seen in vivo. Further purification and characterization of this enzymatic activity should now be possible.
Motion compensated compression of computer animation frames (1993)

This paper presents a new lossless compression algorithm for computer animation image sequences. The algorithm uses transformation information available in the animation script and floating point depth and object number information stored at each pixel to perform highly accurate motion prediction with very low computation. The geometric data, i.e., the depth and object number, is very efficiently compressed using motion prediction and a new technique called direction coding, typically to 1 to 2 bits per pixel. The geometric data is also useful in z-buffer image compositing and this new compression algorithm offers a very low storage overhead method for saving the information needed for z-buffer image compositing. The overall compression ratio of the new algorithm, including the geometric data overhead, is compared to conventional spatial linear prediction compression and is shown to be consistently better, by a factor of 1.4 or more, even with large frame-to-frame motion.
Bimetallic aluminium(acen) complexes as catalysts for the synthesis of cyclic carbonates from carbon dioxide and epoxides (2011)

The development of bimetallic μ-oxoaluminium(acen) complexes as highly active catalysts for the synthesis of cyclic carbonates from terminal epoxides and carbon dioxide is described. The resulting homogeneous catalysts are used in batch reactions for the synthesis of cyclic carbonates at room temperature and one atmosphere pressure.
Human liver glucuronate 2-sulphatase. Purification, characterization and catalytic properties (1989)

Human glucuronate 2-sulphatase (GAS), which is involved in the degradation of the glycosaminoglycans heparan sulphate and chondroitin 6-sulphate, was purified almost 2,000,000-fold to homogeneity in 8% yield from liver with a four-step six-column procedure, which consists of a concanavalin A-Sepharose/Blue A-agarose coupled step, a DEAE-Sephacel/octyl-Sepharose coupled step, CM-Sepharose chromatography and gel-permeation chromatography. Although more than 90% of GAS activity had a pI of greater than 7.5, other forms with pI values of 5.8, 5.3, 4.7 and less than 4.0 were also present. The pI greater than 7.5 form of GAS had a native molecular mass of 63 kDa. SDS/polyacrylamide-gel-electrophoretic analysis resulted in two polypeptide subunits of molecular mass 47 and 19.5 kDa. GAS was active towards disaccharide substrates derived from heparin [O-(beta-glucuronic acid 2-sulphate)-(1----4)-O-(2,5)-anhydro[1-3H]mannitol 6-sulphate (GSMS)] and chondroitin 6-sulphate [O-(beta-glucuronic acid 2-sulphate-(1----3)-O-(2,5)-anhydro[1-3H]talitol 6-sulphate (GSTS)]. GAS activity towards GSMS and GSTS was at pH optima of 3.2 and 3.0 respectively with apparent Km values of 0.3 and 0.6 microM respectively and corresponding Vmax values of 12.8 and 13.7 mumol/min per mg of protein respectively. Sulphate and phosphate ions are potent inhibitors of enzyme activity. Cu2+ ions stimulated, whereas EDTA inhibited enzyme activity. It was concluded that GAS is required together with a series of other exoenzyme activities in the lysosomal degradation of glycosaminoglycans containing glucuronic acid 2-sulphate residues.
PAI‐1, a target gene of miR‐143, regulates invasion and metastasis by upregulating MMP‐13 expression of human osteosarcoma (2016)

Despite recent improvements in the therapy for osteosarcoma, 30-40% of osteosarcoma patients die of this disease, mainly due to its lung metastasis. We have previously reported that intravenous injection of miR-143 significantly suppresses lung metastasis of human osteosarcoma cells (143B) in a mouse model. In this study, we examined the biological role and mechanism of miR-143 in the metastasis of human osteosarcoma cells. We identified plasminogen activator inhibitor-1 (PAI-1) as a direct target gene of miR-143. To determine the role of PAI-1 in human osteosarcoma cells, siRNA was transfected into 143B cells for knockdown of PAI-1 expression. An in vitro study showed that downregulation of PAI-1 suppressed cell invasion activity, but not proliferation. Moreover, injection of PAI-1 siRNA into a primary lesion in the osteosarcoma mouse model inhibited lung metastasis compared to control siRNA-injected mice, without influencing the proliferative activity of the tumor cells. Subsequent examination using 143B cells revealed that knockdown of PAI-1 expression resulted in downregulation of the expression and secretion of matrix metalloproteinase-13 (MMP-13), which is also a target gene of miR-143 and a proteolytic enzyme that regulates tumor-induced osteolysis. Immunohistochemical analysis using clinical samples showed that higher miR-143 expressing cases showed poor expression of PAI-1 in the primary tumor cells. All such cases belonged to the lung metastasis-negative group. Moreover, the frequency of lung metastasis-positive cases was significantly higher in PAI-1 and MMP-13 double-positive cases than in PAI-1 or MMP-13 single-positive or double-negative cases (P < 0.05). These results indicated that PAI-1, a target gene of miR-143, regulates invasion and lung metastasis via enhancement of MMP-13 expression and secretion in human osteosarcoma cells, suggesting that these molecules could be potential therapeutic target genes for preventing lung metastasis in osteosarcoma patients.
Structure of laminin variants. The 300-kDa chains of murine and bovine heart laminin are related to the human placenta merosin heavy chain and replace the a chain in some laminin variants. (1991)

A variant of laminin has previously been isolated from murine heart and shown to be distinct from laminin purified from a traditional source, the murine Engelbreth-Holm-Swarm (EHS) tumor (Paulsson, M., and Saladin, K. (1989) J. Biol. Chem. 264, 18726-18732). It contains a novel polypeptide chain designated as 300 kDa, which is not found in laminin from the EHS tumor. In the present study, heart laminin was purified from bovine tissue and shown to be structurally and immunochemically closely related to the murine protein. Further, heart laminins were compared with merosin, a laminin-like protein isolated from human placenta (Ehrig, K., Leivo, I., Argraves, W. S., Ruoslahti, E., and Engvall, E. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 3264-3268). The 300-kDa chain of bovine heart laminin cross-reacted with the heavy chain of merosin, showing that these polypeptides are closely related, albeit from different species. Heart laminin is more resistant to proteolysis than laminin derived from the EHS tumor. A large fragment could be prepared by digestion with thermolysin, which consisted of an almost intact long arm structure and variably long, residual short arm structures. Analysis of its structure shows that the 300-kDa heavy chain is disulfide-bonded to the B1 and B2 chains in the center of the laminin cross and forms the long arm together with these chains. It thereby replaces the A chain, well known from tumor sources, in the laminin structure.
Autorefraction/Autokeratometry and Subjective Refraction in Untreated and Photorefractive Keratectomy-Treated Eyes (1997)

Refractive and keratometric readings obtained with the automated devices tested in this study varied more from those obtained with subjective methods when the eyes examined had been treated with PRK. Corneal topographical changes inherent in the wound-healing process may influence objective measurement with automated devices.
Urea metabolism in the hibernating black bear. (1978)

A telephone message from Elmer Payne explains that Postmaster General Henry C. Payne has fallen ill and Senator N. B. Scott will take over Payne's place in running the Republican National Convention.
Linear mass reflectron with a laser photoionization source for time-of-flight mass spectrometry (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLinear mass reflectron with a laser photoionization source for time-of-flight mass spectrometryD. M. Lubman, W. E. Bell, and M. N. KronickCite this: Anal. Chem. 1983, 55, 8, 1437–1440Publication Date (Print):July 1, 1983Publication History Published online1 May 2002Published inissue 1 July 1983https://doi.org/10.1021/ac00259a062RIGHTS & PERMISSIONSArticle Views136Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (570 KB) Get e-Alerts Get e-Alerts
The effects of clozapine on cognitive functioning in treatment- resistant schizophrenic patients (1997)

This study examined the cognitive functioning of 10 treatment-resistant schizophrenic patients after a minimum of a 1-year trial on clozapine. Results indicated significant improvements on prorated Wechsler Adult Intelligence Scale-Revised (WAIS-R) Full Scale, Verbal, and Performance IQs and on the WAIS-R Similarities and Digit Symbol subtests. A trend was also found for improvement on the Wisconsin Card Sorting Test. It was concluded that clozapine treatment is associated with global cognitive improvements in patients with treatment-resistant schizophrenia. Clozapine treatment may also improve performance on the Wisconsin Card Sorting Test in a subset of patients. Improvement did not appear to be related to the reduction of any cognitive effects of the typical neuroleptics upon their discontinuation.
Photosensitive Glass and Glass-Ceramics (2016)

This book will discuss how glass and glass ceramic interact with light, both transiently and permanently. Ways that light permanently alter the properties of glass and glass ceramic like the color, refractive index, and mechanical and chemical behaviors will be included. Each photochromatic phenomenon will be discussed in detail from the physical and chemical origin to the method fabrication and ultimately to their utilization.
Neural Network Simulation and the Prediction of Corporate Outcomes: Some Empirical Findings (1995)

Neural Networks (NN's) involve an innovative method of simulating and analysing complex and constantly changing systems of relationships. Originally developed to mimic the neural architecture and functioning of the human brain, NN techniques have recently been applied to many different business fields and have demonstrated a capability to solve complex problems. This paper investigates the use of NN techniques as a tool for the modelling and prediction of corporate bankruptcy and other corporate outcomes. The within and out-of-sample accuracy of trained NNs are compared with those of standard logit and multilogit techniques. The results of the study suggest that, from a pure predictive point of view, NN simulation produces a higher predictive accuracy and is more robust than conventional logit and multilogit models.
Clinicopathological Significance of Mesothelin Expression in Invasive Breast Cancer (2012)

Lymph node status, tumour size, HER2/neu and mesothelin protein levels in breast cancer cells were independent prognostic factors. Mesothelin could be useful as a prognostic marker of overall survival in invasive breast cancer.
Pharmacological and rAAV Gene Therapy Rescue of Visual Functions in a Blind Mouse Model of Leber Congenital Amaurosis (2005)

Intraocular gene therapy and pharmacologic bypass provide highly effective and complementary means for restoring retinal function in this animal model of human hereditary blindness. These complementary methods offer hope of developing treatment to restore vision in humans with certain forms of hereditary congenital blindness.
Expression and secretion of TGF-beta isoforms and expression of TGF-beta-receptors I, II and III in normal and neoplastic human breast. (1999)

We investigated gene expression of the TGF-beta signalling system (including peptides and receptors) in normal and malignant breast tissue. Additionally, gene and protein expression was determined in a series of primary epithelial and stromal cultures derived from these tissues. TGF-beta isoforms and their receptors were expressed by both tissue sets, however the percentage of samples expressing each transcript varied. In normal breast, both TGF-beta1 and TGF-beta3 were found in most samples (88 and 89% respectively), with fewer expressing TGF-beta2 (68%). A similar pattern was evident in the tumours. Type I receptor of TGF-beta was constitutively expressed in normal breast and observed in most tumours (90%). Type II and III receptors of TGF-beta were expressed less frequently, although the type II receptor was mainly expressed by tumours (P=0. 0075). All primary cultures produced TGF-beta1 and TGF-beta2. Comparing respective cell populations, tumour stromal cells produced significantly more TGF-beta1 than those derived from normal breast (P<0.0001). Linear regression analysis showed stromal cultures derived from breast tumours exhibited a strong positive correlation (r=0.976) in the production of TGF-beta1 and TGF-beta2. Thus, TGF-beta and TGF-beta-receptors are widely and differentially expressed by normal and malignant breast and secretion of this peptide by epithelial and stromal cultures, in particular those derived from tumours, confirms its potential as an autocrine/paracrine regulator in breast cancer.