gebhart/patrepeval huggingface dataset:
Cholesteric Films as Optical Filters (1971)

A new class of large-area optical filters based on the special reflection properties of cholesteric liquid crystals is described. Several filtering functions are provided by various combinations of liquid-crystal films, polarizers, and retardation plates.
Epidemiology of gout: is the incidence rising? (2002)

Our results indicate that the incidence of primary gout has increased significantly over the past 20 years. While this increase might be a result of improved ascertainment of atypical gout, it may also be related to other, as yet unidentified, risk factors.
Reconstruction of absent lacrimal puncta. (1979)

A previously described technique to isolate absent lacrimal puncta is reemphasized. The procedure has been modified, whereby the entire punctal and vertical canalicular walls are removed with a Holth sclerectomy punch and by silastic intubation of the passages. This has led to increased permanent patency of the lacrimal puncta and a greater, successful resolution of epiphora. A modified pigtail probe with a narrowed tip has been developed to facilitate passage of the silastic tubing without it slipping off the probe.
Identification and Localization of the Gene Cluster Encoding Biosynthesis of the Antitumor Macrolactam Leinamycin in Streptomyces atroolivaceus S-140 (2002)

Leinamycin (LNM), produced by Streptomyces atroolivaceus, is a thiazole-containing hybrid peptide-polyketide natural product structurally characterized with an unprecedented 1,3-dioxo-1,2-dithiolane moiety that is spiro-fused to a 18-member macrolactam ring. LNM exhibits a broad spectrum of antimicrobial and antitumor activities, most significantly against tumors that are resistant to clinically important anticancer drugs, resulting from its DNA cleavage activity in the presence of a reducing agent. Using a PCR approach to clone a thiazole-forming nonribosomal peptide synthetase (NRPS) as a probe, we localized a 172-kb DNA region from S. atroolivaceus S-140 that harbors the lnm biosynthetic gene cluster. Sequence analysis of 11-kb DNA revealed three genes, lnmG, lnmH, and lnmI, and the deduced product of lnmI is characterized by domains characteristic to both NRPS and polyketide synthase (PKS). The involvement of the cloned gene cluster in LNM biosynthesis was confirmed by disrupting the lnmI gene to generate non-LNM-producing mutants and by characterizing LnmI as a hybrid NRPS-PKS megasynthetase, the NRPS module of which specifies for L-Cys and catalyzes thiazole formation. These results have now set the stage for full investigations of LNM biosynthesis and for generation of novel LNM analogs by combinatorial biosynthesis.
Actuators Coupled Design Based Adaptive Backstepping Control of Air-breathing Hypersonic Vehicle**This work is supported by National Natural Science Foundation of China under Grants 61333008 and 61273153. (2015)

In this paper, we study the robust design of the flight control system for the longitudinal dynamics of air-breathing hypersonic vehicles. First, the longitudinal dynamics model is divided into the velocity subsystem and the altitude subsystem. An adaptive controller is developed based on dynamic inversion method for the former subsystem, and an adaptive backstepping controller is proposed with coupled elevator and canard actuators for the latter, where the coupled design idea makes the control efficiency optimization. Then, the stability analysis shows that the closed system is asymptotically stable under certain conditions, and all the states of the closed-loop system are within a given flight envelope, which means that we realize the restriction control of the states of the vehicles. Finally, simulation illustrates the proposed method. Our designs differ with the published results in 4 aspects. Besides the couples actuators design, we give the specific controller solvers and control parameters selection approaches, and the controller designed doesn't contain the uncertain parameters which are cancelled adaptively.
Use of Adsol® preservation solution for prolonged storage of low viscosity AS‐1 red blood cells (1984)

A new red cell preservation solution is described in which the red cells may be stored for 49 d with greater than 75% mean post-transfusion recovery. Blood is drawn into Anticoagulant Citrate Phosphate Dextrose Solution, centrifuged, the plasma removed and the cells resuspended in 100 ml of a solution containing saline, adenine, dextrose and mannitol (Adsol Preservation Solution, Fenwal Laboratories, Deerfield, Illinois). The final product, AS-1 Red Blood Cells, has a haematocrit of approximately 0.60 and flow properties that are similar to those of whole blood. After storage, red cell haemolysis is minimal and erythrocyte adenosine triphosphate is well preserved. This study documents that red cells may be stored in a protein-poor electrolyte medium for periods of 49 d with good post-transfusion survival.
Multiple Forms of bFGF: Differential Nuclear and Cell Surface Localization (1991)

The single copy gene for human basic fibroblast growth factor (bFGF) has been shown to encode not one but multiple proteins of 24, 23, 22 and 18 kD. Although bioactivities of the 18 kD protein are currently used to define bFGF gene function, it is not yet known if the three larger proteins have these same bioactivities or whether they will serve to define new bFGF gene functions. In this report we present a comparative study describing the de novo synthesis, transport, processing and intracellular location of individual bFGF isoforms. Data from cDNA mutagenesis and COS cell expression experiments show that individual isoforms are differentially localized to either the cell surface or to the nucleus. The 24, 23 and 22 kD proteins (CUG-mediated initiation) exclusively localize in the nucleus while the 18 kD protein (AUG-mediated initiation) is preferentially exported onto the cell surface, but is not released into the surrounding culture medium. Specific CUG or AUG translation initiation codons are necessary and sufficient for the synthesis of each isoform examined and thereby, indirectly, mediate differential localization. Since bFGF does not contain the characteristic signals predicted for cell surface or nuclear targeting, our continuing studies will either unmask its functionally equivalent domain(s) or will identify the requisite participation of yet unknown cellular components.
Calcium Carbonate Gel Therapy for Hydrofluoric Acid Burns of the Hand (1990)

Hydrofluoric acid is used extensively as an industrial cleaning agent for metals and glass. Many workers are injured by cutaneous contact of the acid with exposed skin surfaces, particularly hands. Hydrofluoric acid burns are characterized by delayed onset of symptomatology with skin ulceration, and severe pain may be of extended duration. Treatment of hydrofluoric acid burns traditionally has consisted of local infiltration or intraarterial injections of calcium solutions. These injections are painful and frequently require retreatment. A new treatment utilizing a topical gel of calcium carbonate is described. Nine patients have been treated for hydrofluoric acid burns of the hand with calcium carbonate gel applied topically and covered with occlusive glove dressings. A gel slurry is compounded from calcium carbonate tablets and K-Y Jelly. Fingernails of the affected fingers are removed if a subungual burn is obvious. The gel is put into a surgeon's glove and placed over the burned hand. The patient replaces the glove and slurry every 4 hours for 24 hours. After the first day, the glove is discontinued unless there is resumption of painful symptoms. Full range of motion is encouraged during this interval. The calcium carbonate gel technique was successfully utilized in nine patients with no further need for injection therapy. In these patients, pain relief was obtained within 4 hours of treatment, with no further progression of skin ulceration. No reconstructive procedures were required in any patient, and only one patient did not return to full-duty work within 1 week. There were no long-term sequelae from burns treated with this topical therapy, except one patient, who presenting 24 hours after the burn, developed a digital tip neuroma that was excised.
Effect of Deutetrabenazine on Chorea Among Patients With Huntington Disease (2016)

clinicaltrials.gov Identifier: NCT01795859.
Dexmedetomidine Produces a Hypnotic–Anesthetic Action in Rats via Activation of Central Alpha-2 Adrenoceptors (1989)

Dexmedetomidine, a highly selective and potent alpha-2 adrenoceptor agonist, reduces halothane anesthetic requirements by over 90% in rats. The present study examined whether dexmedetomidine produces a hypnotic-anesthetic action in rats. Dexmedetomidine induced a hypnotic-anesthetic state in rats characterized by loss of righting reflex at doses greater than or equal to 0.1 mg/kg. This response was dose-dependent between 0.1 and 3 mg/kg. Alpha-2 adrenoceptor antagonists that cross the blood-brain barrier (antipamezole and idazoxan) decreased the hypnotic-anesthetic action of dexmedetomidine in a dose-dependent fashion. In contrast, the alpha-2 antagonist, L-659,066, which does not penetrate into the CNS did not affect dexmedetomidine-induced hypnosis. Antagonists for the other adrenoceptors not only failed to reduce the hypnotic-anesthetic action of dexmedetomidine but in some cases even potentiated this effect. Thus, prazosin, an alpha-1 adrenoceptor antagonist, significantly enhanced the hypnotic-anesthetic property of dexmedetomidine. Antagonists with beta-2 receptor blocking properties also enhanced dexmedetomidine-induced hypnosis. Selective beta-1 receptor antagonists did not affect the hypnotic action of dexmedetomidine. These results suggest that dexmedetomidine produces a hypnotic-anesthetic action in rats via activation of central alpha-2 adrenoceptors.
Service Recommendation in an Evolving Ecosystem: A Link Prediction Approach (2013)

Services computing is playing a critical role in recent years in many fields and we observe a rapidly increasing number of web accessible services and their compositions nowadays. However, our earlier empirical study reveals that, overall the public available services are under-utilized, and when they are used, they are used mostly in an isolated manner. This phenomenon inspires us to further explore a methodology to help consumers understand the usage pattern of the service ecosystem, including interactions among services, and the evolution of these interactions. Based on the derived usage pattern, this methodology also introduces a service recommendation method that suggests both services and their compositions, in a time-sensitive manner. We firstly construct an evolution network model from the historical usage of the services in the ecosystem. Then a rank-aggregation-based link prediction method is proposed to predict the evolution of the ecosystem. Based on this link prediction method, we can recommend services and compositions of interest to service developers. Through an experiment on the real-world mashup-service ecosystem, i.e., Programmable Web, we demonstrated that our approach can effectively recommend services and compositions with better precision than the methods we compared.
Positive Regulation in the Gram-Positive Bacterium: Bacillus subtilis (1992)

International audience
Cell-Produced α-Synuclein Is Secreted in a Calcium-Dependent Manner by Exosomes and Impacts Neuronal Survival (2010)

alpha-Synuclein is central in Parkinson's disease pathogenesis. Although initially alpha-synuclein was considered a purely intracellular protein, recent data suggest that it can be detected in the plasma and CSF of humans and in the culture media of neuronal cells. To address a role of secreted alpha-synuclein in neuronal homeostasis, we have generated wild-type alpha-synuclein and beta-galactosidase inducible SH-SY5Y cells. Soluble oligomeric and monomeric species of alpha-synuclein are readily detected in the conditioned media (CM) of these cells at concentrations similar to those observed in human CSF. We have found that, in this model, alpha-synuclein is secreted by externalized vesicles in a calcium-dependent manner. Electron microscopy and liquid chromatography-mass spectrometry proteomic analysis demonstrate that these vesicles have the characteristic hallmarks of exosomes, secreted intraluminar vesicles of multivesicular bodies. Application of CM containing secreted alpha-synuclein causes cell death of recipient neuronal cells, which can be reversed after alpha-synuclein immunodepletion from the CM. High- and low-molecular-weight alpha-synuclein species, isolated from this CM, significantly decrease cell viability. Importantly, treatment of the CM with oligomer-interfering compounds before application rescues the recipient neuronal cells from the observed toxicity. Our results show for the first time that cell-produced alpha-synuclein is secreted via an exosomal, calcium-dependent mechanism and suggest that alpha-synuclein secretion serves to amplify and propagate Parkinson's disease-related pathology.
Single-Sideband Transmission by Envelope Elimination and Restoration (1952)

A new type of single-sideband transmitter is described which does not require the use of linear radio-frequency amplifiers. Amplification is accomplished by a process in which the phase-modulation component of the single-sideband wave is amplified by means of Class-C amplifiers, and the amplitude envelope is restored at the final amplifier. Experimental results show performance equal to or better than conventional linear radio-frequency amplifier practices. The over-all efficiency is approximately the same as that of a double-sideband amplitude-modulated transmitter. This system is especially suitable for high-power operation.
Emergence of Zaire Ebola Virus Disease in Guinea (2014)

In March 2014, the World Health Organization was notified of an outbreak of a communicable disease characterized by fever, severe diarrhea, vomiting, and a high fatality rate in Guinea. Virologic investigation identified Zaire ebolavirus (EBOV) as the causative agent. Full-length genome sequencing and phylogenetic analysis showed that EBOV from Guinea forms a separate clade in relationship to the known EBOV strains from the Democratic Republic of Congo and Gabon. Epidemiologic investigation linked the laboratory-confirmed cases with the presumed first fatality of the outbreak in December 2013. This study demonstrates the emergence of a new EBOV strain in Guinea.
An Anti-Interleukin 8 Monoclonal Antibody That Interferes with the Binding of Interleukin 8 to Cellular Receptors and the Activation of Human Blood Neutrophils (1995)

Interleukin 8 (IL-8) is a proinflammatory cytokine produced by a wide variety of cells. Interleukin 8 acts as a neutrophil activator and chemotactic factor. In the current studies, we examined the properties of a monoclonal antibody against human IL-8. The estimated affinity of the antibody was 1.74 x 10(7) liters/mol. The antibody interfered with the binding of radiolabeled recombinant human IL-8 (rhIL-8) to human blood neutrophils (IC50 = 3 x 10(-7) M, at an IL-8 concentration of 2.4 nM). Neutrophil degranulation elicited by 5 x 10(-6)-4 x 10(-8) M rhIL-8 was blocked by the antibody at three-fold molar excess. However, a higher concentration of anti-IL-8 antibody was needed to suppress the chemotactic activity of rhIL-8. The inhibition of neutrophil chemotaxis triggered by 2 x 10(-7)-2 x 10(-9) M rhIL-8 required 6 x 10(-5) M antibody. Similarly, a 300-fold molar excess of anti-IL-8 antibody [10(-5) M] was necessary to abrogate the increase in cytosolic free calcium in neutrophils stimulated with 4 x 10(-8) M rhIL-8. In addition, epitope analysis using synthetic peptides corresponding to different regions of the IL-8 molecule showed that peptide consisting of residues 44-72 (corresponding to the C-terminal of the IL-8 molecule) competed with the antibody for binding to rhIL-8. Because IL-8 is an important inflammatory mediator in several human diseases, anti-IL-8 antibodies may have pharmacological potential.
A simulation tool for hybrid-cooperative positioning (2011)

We propose a simulation tool able to test hybrid GNSS-terrestrial and cooperative positioning algorithms that fuse both pseudorange measurements from satellites and range estimates between terrestrial receivers. In particular, the tool simulates devices belonging to a peer-to-peer (P2P) network where peers cooperate by exchanging data in order to improve both positioning accuracy and availability. More specifically, the simulation tool generates range and pseudorange measurements by employing realistic propagation models and GPS orbits. Furthermore, the simulation tool allows the user to test different algorithms based on Bayesian and least squares methods in two realistic GNSS challenged scenarios namely indoor and urban canyon. Finally, the simulator take into account communication and mobility aspects allowing the user to evaluate their impact on the final positioning accuracy.
Caracterización de canales de ganado bovino en los valles centrales de Oaxaca (2005)

Bovines slaughtered at the Central Oaxaca Valleys in Mexico show high diversity due to their source and production systems. The objective of the present study was to grade slaughtered cattle in this region. A total of 1,375 carcasses were valuated by breed, age, origin, production system, dorsal fat depth, marbling, conformation and weight. Ninety five percent of the carcasses evaluated came from tropicalre as and 75 % of them were reared under grazing conditions. Slaughtered animals were predominantly Zebu x Brown Swiss (37 %) and Zebu x Holstein (16 %) crosses, three to four years old. Bulls from the humid tropics showed the higher carcass weight (309.4 kg P<0.05). Zebu, Zebu x Creole and Zebu x Brown Swiss bulls showed the higher carcass weights (325.8, 301.6 and 297.8 kg, respectively). Bullocks and bulls from the humid tropics showed the higher rate of carcasses with good conformation (P<0.05) as well as those from feed lots and grazing plus supplement (85.2 and 78.8 %, respectively). Breed has strong influence on growth and carcass composition, however, after genetics, the production system, feeding and maturity are determinants of yield and carcass quality.
Characterization of a human T cell-specific chimeric antibody (CD7) with human constant and mouse variable regions. (1989)

A chimeric human-mouse anti-T lymphocyte mAb (CHT2; SDZ 214-380) has been constructed by cloning the variable region exons of both the L and H chains from the murine hybridoma RFT2 which have CD7 specificity and reactivity with a 40-kDa Ag. The variable L chain exon was joined to the human C kappa, and the variable H chain exon was joined to the human IgG1 region exon encoding the human allotype nGlm(z), nGlm(a). The gene constructs were introduced by electroporation into SP2/0, a non-Ig-producing murine myeloma. The identical tissue reactivity of the newly made CHT2 and the original murine RFT2 mAb (CD7) was confirmed by blocking experiments as well as by immunohistology and flow cytometry. Because this new mAb may have clinical use, the CD7 Ag expression of T lineage cells has also been quantitated in double and triple immunofluorescence assays in combinations with mAb to restricted forms of leukocyte common Ag that designate unprimed (CD45R+) and primed T lymphocyte populations (UCHL1+). CHT2 shows very strong reactivity with large thymic blast cells and cortical thymocytes from which T-ALL originates. Strong staining is seen on CD45R+ unprimed "virgin" T lymphocytes, whereas the expression on UCHL1+ primed "memory" cell types is weaker unless these cells are reactivated by mitogens or Ag. Thus CHT2 may spare a substantial population of resting memory T cells which is relevant to its potential therapeutic use. In addition the chimeric antibody had a greater in vitro antibody dependent cytotoxicity and a prolonged half-life (4.2 to 5.0 days) in Rhesus monkeys.
ELECTRON MICROSCOPIC STUDIES OF INDUCED CARTILAGE DEVELOPMENT AND CALCIFICATION (1967)

Cultured, human, amniotic cells (FL strain) injected into the thigh muscles of cortisone-conditioned mice proliferated to form discrete colonies which, over a period of 5 days, became invested by numerous fibroblasts. Cartilage cells and matrix appeared within the fibroblastic zones during the succeeding 2-4 days. Cartilage matrix calcified within 12 days following FL-cell injection. Cartilage cells closely resembled fibroblasts from which they appeared to be derived, and were readily distinguished from FL cells by their prominent ergastoplasm and Golgi complexes. Cartilage matrix was composed of a distinctive feltwork of randomly arranged, collagen fibrils ( approximately 600 A axial period and approximately 250 A width) from which small electron-opaque, leaflike matrix particles extended. Matrix calcification occurred with the deposition of radially arranged needle-like structures resembling hydroxyapatite. Dense centers were often identified within these clusters. Examination of heavily calcified areas revealed confluent masses of apatite-like material. In general, the fine structure of induced cartilage formation and calcification resembled that of cartilage development and calcification as previously described in the normal epiphysis.
A Structure−Function Study of a Proton Transport Pathway in the γ-Class Carbonic Anhydrase from Methanosarcina thermophila (2000)

Four glutamate residues in the prototypic gamma-class carbonic anhydrase from Methanosarcina thermophila (Cam) were characterized by site-directed mutagenesis and chemical rescue studies. Alanine substitution indicated that an external loop residue, Glu 84, and an internal active site residue, Glu 62, are both important for CO(2) hydration activity. Two other external loop residues, Glu 88 and Glu 89, are less important for enzyme function. The two E84D and -H variants exhibited significant activity relative to wild-type activity in pH 7.5 MOPS buffer, suggesting that the original glutamate residue could be substituted with other ionizable residues with similar pK(a) values. The E84A, -C, -K, -Q, -S, and -Y variants exhibited large decreases in k(cat) values in pH 7.5 MOPS buffer, but only exhibited small changes in k(cat)/K(m). These same six variants were all chemically rescued by pH 7.5 imidazole buffer, with 23-46-fold increases in the apparent k(cat). These results are consistent with Glu 84 functioning as a proton shuttle residue. The E62D variant exhibited a 3-fold decrease in k(cat) and a 2-fold decrease in k(cat)/K(m) relative to those of the wild type in pH 7.5 MOPS buffer, while other substitutions (E62A, -C, -H, -Q, -T, and -Y) resulted in much larger decreases in both k(cat) and k(cat)/K(m). Imidazole did not significantly increase the k(cat) values and slightly decreased the k(cat)/K(m) values of most of the Glu 62 variants. These results indicate a primary preference for a carboxylate group at position 62, and support a proposed catalytic role for residue Glu 62 in the CO(2) hydration step, but do not definitively establish its role in the proton transport step.