gebhart/patrepeval huggingface dataset:
Automated mass immunization of poultry: the prospect for nonreplicating human adenovirus-vectoredin ovovaccines (2007)

Automated in ovo vaccination is an efficient method for mass immunization of poultry. Although in ovo vaccination has been used to mass immunize chickens against several infectious diseases, there are common poultry diseases for which in ovo-compatible vaccines are not commercially available. It was recently demonstrated that in ovo administration of a nonreplicating human adenovirus vector encoding an avian influenza virus hemagglutinin induced protective immunity against highly pathogenic avian influenza. The advantages of this new class of poultry vaccine include in ovo delivery of a wide variety of pathogen-derived antigens, high potency in a single-dose regimen, rapid production in response to increased demand, no replication of the vector, no pre-existing immunity to human adenovirus in chickens, compatibility with automated in ovo administration and no interference with epidemiological surveys of natural infections.
Glypican-1 is overexpressed in human breast cancer and modulates the mitogenic effects of multiple heparin-binding growth factors in breast cancer cells. (2001)

Glypicans are a family of glycosylphosphatidylinositol-anchored cell surface heparan sulfate proteoglycans implicated in the control of cellular growth and differentiation. Here we show that glypican-1 is strongly expressed in human breast cancers, whereas expression of glypican-1 is low in normal breast tissues. In contrast, the expression of glypican-3 and -4 is only slightly increased in breast cancers by comparison with normal breast tissues, and glypican-2 and -5 are below the level of detection by Northern blotting in both normal and cancer samples. Treatment of MDA-MB-231 and MDA-MB-468 breast cancer cells with phosphoinositide-specific phospholipase-C abrogated the mitogenic response to two heparin-binding growth factors, heparin-binding epidermal growth factor-like growth factor and fibroblast growth factor 2. Stable transfection of these cells with a glypican-1 antisense construct markedly decreased glypican-1 protein levels and the mitogenic response to the same heparin-binding growth factors, as well as that to heregulin alpha, heregulin beta, and hepatocyte growth factor. Syndecan-1 was also expressed at high levels in both breast cancer tissues and breast cancer cells when compared with normal breast tissues. There was a good correlation between glypican-1 and syndecan-1 expression in the tumors. However, clones expressing the glypican-1 antisense construct did not exhibit decreased syndecan-1 levels, indicating that loss of responsiveness to heparin-binding growth factors in these clones was not due to altered syndecan-1 expression. Furthermore, 8 of 10 tumors with stage 2 or 3 disease exhibited high levels of glypican-1 by Northern blot analysis. In contrast, low levels of glypican-1 mRNA were evident in 1 of 10 tumors with stage 2 or 3 disease and in 9 of 10 tumors with stage 1 disease. Taken together, these data suggest that glypican-1 may play a pivotal role in the ability of breast cancer cells to exhibit a mitogenic response to multiple heparin-binding growth factors and may contribute to disease progression in this malignancy.
Inclusion Compounds (2000)

Abstract The article contains sections titled: 1. Introduction 2. Classification 2.1. Inorganic Hosts 2.2. Organic Hosts 2.3. Biochemical Inclusion 3. Uses 3.1. Zeolites 3.2. Cyclodextrins
Effect of protein binding on high performance liquid chromatography analysis of drugs with an internal-surface reversed-phase silica column. (1989)

The effect of protein binding on the high performance liquid chromatography (HPLC) elution profile of drugs injected directly onto an internal-surface reversed-phase (ISRP) silica column was investigated. When a relatively large volume (greater than or equal to 100 microliters) of the sample solution containing warfarin and bovine serum albumin (BSA) was applied directly onto the ISRP column and elution was carried out with the mobile phase containing an organic modifier, two distinct peaks, both due to warfarin, appeared separated from the protein peak. The peak splitting was not observed in the case of antipyrine-BSA mixed solution, where the protein binding is weak. It was found that the drug bound at the strong-binding site on the BSA molecule was released slowly during the process of chromatography and was eluted as the first peak with a shorter retention time, while the drug bound at the weak-binding site was released quickly, and was eluted as the second peak together with the free drug. The warfarin-BSA interaction at the strong-binding site was evaluated, under minimum influence from the other binding site, from the warfarin concentration obtained from the first peak and the free warfarin concentration determined by the ultrafiltration method.
Time Lens-Based Optical Fourier Transformation for All-Optical Signal Processing of Spectrally-Efficient Data (2016)

We review recent progress in the use of time lens-based optical Fourier transformation for advanced all-optical signal processing. A novel time lens-based complete optical Fourier transformation (OFT) technique is introduced. This complete OFT is based on two quadratic phase-modulation stages using four-wave mixing, separated by a dispersive medium, which enables time-to-frequency and frequency-to-time conversions simultaneously, thus performing an exchange between the temporal and spectral profiles of the input signal. Using the proposed complete OFT, several advanced all-optical signal processing schemes for spectrally-efficient systems and networks have been achieved, including all-optical generation, detection and format conversion of spectrally-efficient signals. The spectrally-efficient signals in this paper mainly refer to efficiently multiplexed signals with a high symbol rate per Hz, such as orthogonal frequency division multiplexing, Nyquist wavelength-division multiplexing (Nyquist-WDM), and Nyquist optical time division multiplexing (Nyquist-OTDM) signals.
2000 CDC Growth Charts for the United States: methods and development. (2002)

The 2000 CDC growth charts were developed with improved data and statistical procedures. Health care providers now have an instrument for growth screening that better represents the racial-ethnic diversity and combination of breast- and formula-feeding in the United States. It is recommended that these charts replace the 1977 NCHS charts when assessing the size and growth patterns of infants, children, and adolescents.
A multi-class predictor based on a probabilistic model: application to gene expression profiling-based diagnosis of thyroid tumors (2006)

Abstract Background Although microscopic diagnosis has been playing the decisive role in cancer diagnostics, there have been cases in which it does not satisfy the clinical need. Differential diagnosis of malignant and benign thyroid tissues is one such case, and supplementary diagnosis such as that by gene expression profile is expected. Results With four thyroid tissue types, i.e., papillary carcinoma, follicular carcinoma, follicular adenoma, and normal thyroid, we performed gene expression profiling with adaptor-tagged competitive PCR, a high-throughput RT-PCR technique. For differential diagnosis, we applied a novel multi-class predictor, introducing probabilistic outputs. Multi-class predictors were constructed using various combinations of binary classifiers. The learning set included 119 samples, and the predictors were evaluated by strict leave-one-out cross validation. Trials included classical combinations, i.e., one-to-one, one-to-the-rest, but the predictor using more combination exhibited the better prediction accuracy. This characteristic was consistent with other gene expression data sets. The performance of the selected predictor was then tested with an independent set consisting of 49 samples. The resulting test prediction accuracy was 85.7%. Conclusion Molecular diagnosis of thyroid tissues is feasible by gene expression profiling, and the current level is promising towards the automatic diagnostic tool to complement the present medical procedures. A multi-class predictor with an exhaustive combination of binary classifiers could achieve a higher prediction accuracy than those with classical combinations and other predictors such as multi-class SVM. The probabilistic outputs of the predictor offer more detailed information for each sample, which enables visualization of each sample in low-dimensional classification spaces. These new concepts should help to improve the multi-class classification including that of cancer tissues.
Effect of Adenovirus Gene Transfer Vectors on the Immunologic Functions of Mouse Dendritic Cells (2002)

To address the effect of adenovirus (Ad) gene transfer vector transduction on the diverse functions of dendritic cells, we used an Ad vector encoding no transgene (AdNull) to transduce mouse bone-marrow-derived dendritic cells (BMDC). Initial experiments using an Ad vector encoding a marker gene (AdGFP, jellyfish green fluorescent protein) showed that the optimal ratio of infectious Ad particles to each cell was 100, when both transgene expression and resultant BMDC viability were taken into account. Exposure to AdNull resulted in upregulation of both surface activation markers (CD40, MHC class II, B7.1, B7.2, ICAM-1) and IL-12 expression by BMDC. AdNull activation of BMDC was observed in multiple strains of mice. Despite this, AdNull-transduced BMDC displayed only modestly impaired antigen uptake ability, as demonstrated in macropinocytosis and phagocytosis assays, in vitro. However, Ad-modified BMDC migrated to regional lymph nodes five times more efficiently than sham-transduced BMDC in vivo. In addition, Ad transduction significantly enhanced the ability of BMDC to present a model peptide antigen to T-lymphocyte hybridoma cells at low BMDC:T cell ratios. We conclude that Ad modification, in and of itself, induces a state of activation in mouse BMDC. This activation, albeit mild compared with that induced by other stimuli, produces measurable effects of the specific immunological functions of these antigen-presenting cells.
Novel Ferroelectric and Electroclinic Organosiloxane Liquid Crystals (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel Ferroelectric and Electroclinic Organosiloxane Liquid CrystalsJ. Naciri, J. Ruth, G. Crawford, R. Shashidhar, and B. R. RatnaCite this: Chem. Mater. 1995, 7, 7, 1397–1402Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/cm00055a019https://doi.org/10.1021/cm00055a019research-articleACS PublicationsRequest reuse permissionsArticle Views314Altmetric-Citations83LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Detection of Prostate Cancer Using Serum Proteomics Pattern in a Histologically Confirmed Population (2004)

Serum proteomics patterns may potentially aid in the early detection of prostate cancer.
Hybrid compression of video with graphics in DTV communication systems (2000)

Advanced broadcast manipulation of TV sequences and enhanced user interfaces for TV systems have resulted in an increased amount of pre- and post-editing of video sequences, where graphical information is inserted. However, in the current broadcasting chain, there are no provisions for enabling an efficient transmission/storage of these mixed video and graphics signals and, at this emerging stage of DTV systems, introducing new standards is not desired. Nevertheless, in the professional video communication chain between content provider and broadcaster and locally, in the DTV receiver, proprietary video-graphics compression schemes can be used to enable more efficient transmission/storage of mixed video and graphics signals. For example, in the DTV receiver case this will lead to a significant memory-cost reduction. To preserve a high overall image quality, the video and graphics data require independent coding systems, matched with their specific visual and statistical properties. We introduce various efficient algorithms that support both the lossless (contour, runlength and arithmetic coding) and the lossy (block predictive coding) compression of graphics data. If the graphics data are a-priori mixed with video and the graphics position is unknown at compression time, an accurate detection mechanism is applied to distinguish the two signals, such that independent coding algorithms can be employed for each data-type. In the DTV memory-reduction scenario, an overall bit-rate control completes the system, ensuring a fixed compression factor of 2-3 per frame without sacrificing the quality of the graphics.
Serum soluble IL-2 receptor as a tumor marker in patients with hairy cell leukemia (1988)

Activated T cells synthesize and express a cell membrane-bound receptor for interleukin-2 (IL-2) and have recently been shown to secrete a soluble form of the same receptor. Hairy cell leukemia is a chronic disorder caused by expansion of a clonal population of an unusual mononuclear cell of B cell origin. These cells have previously been shown to express an IL-2 receptor on the cell membrane. The sera of 26 patients with hairy cell leukemia were examined for the presence of a soluble IL-2 receptor before and during therapy with either recombinant interferon alpha-2a or 2′-deoxycoformycin. Before therapy, all patients had markedly elevated levels of this soluble IL-2 receptor ranging from five to 60 times the highest level observed in normal control sera. In individual patients changes in the level during therapy correlated well with clinical assessments of tumor response; levels fell to near the normal range in patients responding to therapy. Patients not responding to interferon alpha had no significant change in the soluble IL-2 receptor level. These results suggest that hairy cells secrete a soluble IL-2 receptor and that serial measurements of the level of this receptor in the serum can be used as a noninvasive means to assess disease response to therapy.
Antigenic analysis of four species of the genus Ehrlichia by use of protein immunoblot (1991)

The antigenic profile of Ehrlichia canis, E risticii, E sennetsu, and E equi was investigated by the use of protein (western) immunoblot technique. Results of analysis of serum from acutely and chronically infected animals indicated that the 4 Ehrlichia species share a unique 25-kD polypeptide in addition to other peptides. Immune sera from dogs inoculated with E canis recognized a wide range of E canis polypeptide antigens, as determined by western blot analysis. A larger number of E sennetsu polypeptides were detected when homologous antiserum and antiserum to E equi were used. The latter antiserum did not recognize antigens of E canis or E risticii. Antisera to E canis, E risticii, and E sennetsu detected E equi antigens. Data indicate that a 25-kD protein is a common antigen among the species of the genus Ehrlichia and that the ascending order of abundance of immunodominant determinants in the 4 species of Ehrlichia studied would be: E risticii----E equi----E sennetsu----E canis. Implications of these findings for diagnosis of ehrlichial infections and prophylaxis are evident.
Synthesis and Size Control of Tetragonal Barium Titanate Nanopowders by Facile Solvothermal Method (2012)

A facile synthetic strategy was implemented to obtain nanosized barium titanate ( BaTiO 3 ) powders with tetragonal structure. The nanoparticles were synthesized using solvothermal process employing diethanolamine and triethanolamine to suppress the particle growth and the as‐prepared nanopowders were characterized using X‐ray diffraction, scanning electron microscopy, and high‐resolution dispersive R aman spectroscopy. It was found that the particle size can be easily tuned by adjusting the experimental parameters while retaining the tetragonality. The average diameters of the particles prepared with and without the organic amines were found to be 80 and 100 nm, respectively. All the synthesized BaTiO 3 nanopowders exhibit a narrow size distribution with a uniform morphology. Rietveld refinement of the XRD patterns and Raman spectra revealed that the synthesized BaTiO 3 nanopowders have tetragonal asymmetry dominant structures. A slight decrease in the tetragonality of the prepared powders with decrease in particle size is attributed to the presence of cubic shell layer and inner defects. The tetragonal‐dominant structure was also confirmed by normalizing the peak area of the Raman spectra.
An evaluation of antigen-driven expansion and differentiation of hapten-specific B lymphocytes purified from aged mice. (1987)

Hapten-specific, trinitrophenyl antigen-binding B cells (TNP-ABC) were purified from inbred strains of mice representative of short-, intermediate-, and long-lived animals. Such populations of B cells were stimulated by either thymus-independent or thymus-dependent antigens in vitro and evaluated for both proliferation and differentiation into antibody-secreting cells. In the thymus-dependent system, the three strains of mice were selected on the basis of all being able to interact appropriately with the same T helper cell line. The results indicate that TNP-ABC purified from aged animals of all three strains responded to both forms of antigenic stimulation similar to TNP-ABC selected from young, littermate control animals. These results are discussed in terms of concepts of intrinsic B cell defects during the aging process.
Identification of Cell Proliferation Zones, Progenitor Cells and a Potential Stem Cell Niche in the Intervertebral Disc Region (2009)

BrdU positive cells were found in comparable numbers at early and late time points in most regions of the anulus fibrosus (AF) and nucleus pulposus demonstrating slow ongoing cell proliferation. In the AF border to ligament zone (AFo) and the perichondrium region (P) a stem cell niche-like pattern was determined (a high number of BrdU positive cells at early time points vs. only a few label retaining cells at later time points). In normal and DD tissue from the 4 investigated species progenitor cell markers were detected. Conclusion. The IVD is a tissue with ongoing slow cell proliferation both in the AF and the nucleus pulposus. The stem cell niche pattern detected in AFo and P can be suggested to play a role for IVD morphology and function. These findings may be of importance for the development of biologic treatment strategies.
Expression patterns of the novel receptor-like tyrosine kinase, DDR, in human breast tumours. (1995)

Using a reverse transcriptase-polymerase chain reaction based differential screening procedure, we have identified the discoidin domain receptor as a protein tyrosine kinase that is expressed in lymph nodes containing breast tumour metastases. By Northern blotting and in situ hybridisation we have demonstrated the expression of the discoidin domain receptor in human primary breast tumour samples, metastasis-containing lymph nodes and a number of normal tissues. Direct comparison of malignant breast and adjacent normal epithelial tissue revealed over expression in the tumour cells.
Human TSLP promotes CD40 ligand-induced IL-12 production by myeloid dendritic cells but maintains their Th2 priming potential (2005)

Interleukin-4 (IL-4), a major T-helper type 2 (Th2) cytokine, primes dendritic cells (DCs) for IL-12 production, suggesting a negative feedback loop to prevent dysregulated Th2 inflammation, such as allergy. We previously showed that human thymic stromal lymphopoietin (TSLP), highly expressed by keratinocytes of atopic dermatitis, activates CD11c(+) DCs to induce the differentiation of naive CD4(+) and CD8(+) T cells into proallergic effectors. Here we show that TSLP primes DCs to produce large amounts of IL-12 after CD40 ligand stimulation, similar to IL-4 priming of DCs. In contrast to IL-4 priming, DCs activated with TSLP and CD40 ligand induce the differentiation of naive CD4(+) T cells into effectors producing both Th1 and Th2 cytokines, a unique profile that is reminiscent of the late phase of allergy. Thus, TSLP is a major regulatory cytokine for IL-12 production by DCs, and TSLP-activated DCs could promote the persistence of Th2 inflammation even in the presence of IL-12-inducing signals.
Experimental study on myocardial protection by adjunct use of carperitide (hANP) in cardiac surgery. (2005)

The study demonstrated that hANP improves ischemic reperfusion injury and suggested that hANP exerts direct myocardial protection against myocardial injury associated with cardiac surgery (cardioplegic arrest while cardiopulmonary bypass).
Effects of cardiotonic steroids on dermal collagen synthesis and wound healing (2008)

We previously reported that cardiotonic steroids stimulate collagen synthesis by cardiac fibroblasts in a process that involves signaling through the Na-K-ATPase pathway (Elkareh et al. Hypertension 49: 215-224, 2007). In this study, we examined the effect of cardiotonic steroids on dermal fibroblasts collagen synthesis and on wound healing. Increased collagen expression by human dermal fibroblasts was noted in response to the cardiotonic steroid marinobufagenin in a dose- and time-dependent fashion. An eightfold increase in collagen synthesis was noted when cells were exposed to 10 nM marinobufagenin for 24 h (P < 0.01). Similar increases in proline incorporation were seen following treatment with digoxin, ouabain, and marinobufagenin (10 nM x 24 h, all results P < 0.01 vs. control). The coadministration of the Src inhibitor PP2 or N-acetylcysteine completely prevented collagen stimulation by marinobufagenin. Next, we examined the effect of digoxin, ouabain, and marinobufagenin on the rate of wound closure in an in vitro model where human dermal fibroblasts cultures were wounded with a pipette tip and monitored by digital microscopy. Finally, we administered digoxin in an in vivo wound healing model. Olive oil was chosen as the digoxin carrier because of a favorable partition coefficient observed for labeled digoxin with saline. This application significantly accelerated in vivo wound healing in rats wounded with an 8-mm biopsy cut. Increased collagen accumulation was noted 9 days after wounding (both P < 0.01). The data suggest that cardiotonic steroids induce increases in collagen synthesis by dermal fibroblasts, as could potentially be exploited to accelerate wound healing.