gebhart/patrepeval huggingface dataset:
Organoborates in new synthetic reactions (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganoborates in new synthetic reactionsAkira SuzukiCite this: Acc. Chem. Res. 1982, 15, 6, 178–184Publication Date (Print):June 1, 1982Publication History Published online1 May 2002Published inissue 1 June 1982https://pubs.acs.org/doi/10.1021/ar00078a003https://doi.org/10.1021/ar00078a003research-articleACS PublicationsRequest reuse permissionsArticle Views2601Altmetric-Citations401LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Confidence Driven Unsupervised Semantic Parsing (2011)

Current approaches for semantic parsing take a supervised approach requiring a considerable amount of training data which is expensive and difficult to obtain. This supervision bottleneck is one of the major difficulties in scaling up semantic parsing. We argue that a semantic parser can be trained effectively without annotated data, and introduce an unsupervised learning algorithm. The algorithm takes a self training approach driven by confidence estimation. Evaluated over Geoquery, a standard dataset for this task, our system achieved 66 % accuracy, compared to 80 % of its fully supervised counterpart, demonstrating the promise of unsupervised approaches for this task. 1
Analogs of Tetrahydroisoquinoline Natural Products That Inhibit Cell Migration and Target Galectin-3 Outside of Its Carbohydrate-binding Site (2008)

Cell migration is central to a number of normal and disease processes. Small organic molecules that inhibit cell migration have potential as both research probes and therapeutic agents. We have identified two tetrahydroisoquinoline natural product analogs with antimigratory activities on Madin-Darby canine kidney epithelial cells: a semisynthetic derivative of quinocarmycin (also known as quinocarcin), DX-52-1, and a more complex synthetic molecule, HUK-921, related to the naphthyridinomycin family. It has been assumed that the cellular effects of reactive tetrahydroisoquinolines result from the alkylation of DNA. We have reported previously that the primary target of DX-52-1 relevant to cell migration appears to be the membrane-cytoskeleton linker protein radixin. Here we extend the analysis of the protein targets of DX-52-1, reporting that the multifunctional carbohydrate-binding protein galectin-3 is a secondary target of DX-52-1 that may also be relevant to the antimigratory effects of both DX-52-1 and HUK-921. All known inhibitors of galectin-3 target its beta-galactoside-binding site in the carbohydrate recognition domain. However, we found that DX-52-1 and HUK-921 bind galectin-3 outside of its beta-galactoside-binding site. Intriguingly HUK-921, although a less potent inhibitor of cell migration than DX-52-1, had far greater selectivity for galectin-3 over radixin, exhibiting little binding to radixin, both in vitro and in cells. Overexpression of galectin-3 in cells led to a dramatic increase in cell adhesion on different extracellular matrix substrata as well as changes in cell-cell adhesion and cell motility. Galectin-3-overexpressing cells had greatly reduced sensitivity to DX-52-1 and HUK-921, and these compounds caused a change in localization of the overexpressed galectin-3 and reversion of the cells to a more normal morphology. The converse manipulation, RNA interference-based silencing of galectin-3 expression, resulted in reduced cell-matrix adhesion and cell migration. In aggregate, the data suggest that DX-52-1 and HUK-921 inhibit a carbohydrate binding-independent function of galectin-3 that is involved in cell migration.
A Mouse Model of Tuberous Sclerosis: Neuronal Loss of Tsc1 Causes Dysplastic and Ectopic Neurons, Reduced Myelination, Seizure Activity, and Limited Survival (2007)

Tuberous sclerosis (TSC) is a hamartoma syndrome caused by mutations in TSC1 or TSC2 in which cerebral cortical tubers and seizures are major clinical issues. We have engineered mice in which most cortical neurons lose Tsc1 expression during embryonic development. These Tsc1 mutant mice display several neurological abnormalities beginning at postnatal day 5 with subsequent failure to thrive and median survival of 35 d. The mice also display clinical and electrographic seizures both spontaneously and with physical stimulation, and some seizures end in a fatal tonic phase. Many cortical and hippocampal neurons are enlarged and/or dysplastic in the Tsc1 mutant mice, strongly express phospho-S6, and are ectopic in multiple sites in the cortex and hippocampus. There is a striking delay in myelination in the mutant mice, which appears to be caused by an inductive neuronal defect. This new TSC brain model replicates several features of human TSC brain lesions and implicates an important function of Tsc1/Tsc2 in neuronal development.
Synthetic gene-transfer vectors (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic gene-transfer vectorsJean Paul BehrCite this: Acc. Chem. Res. 1993, 26, 5, 274–278Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/ar00029a008https://doi.org/10.1021/ar00029a008research-articleACS PublicationsRequest reuse permissionsArticle Views758Altmetric-Citations194LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Hardening COTS software with generic software wrappers (2002)

Numerous techniques exist to augment the security functionality of Commercial Off-The-Shelf (COTS) applications and operating systems, making them more suitable for use in mission-critical systems. Although individually useful, as a group these techniques present difficulties to system developers because they are not based on a common framework which might simplify integration and promote portability and reuse. This paper presents techniques for developing Generic Software Wrappers-protected, non-bypassable kernel-resident software extensions for augmenting security without modification of COTS source. We describe the key elements of our work: our high-level Wrapper Definition Language (WDL), and our framework for configuring, activating, and managing wrappers. We also discuss code reuse, automatic management of extensions, a framework for system-building through composition, platform-independence, and our experiences with our Solaris and FreeBSD prototypes.
Geometry of feasible injection region of power networks (2011)

We investigate the problem of power flow and its implications to the optimization in power networks. To understand how to solve these optimization problems, we look at the injection region of power networks. The injection region of a network is the set of all vectors of power injections, one at each bus, that can be achieved while satisfying the network and operation constraints. If there are no operation constraints, we show the injection region of a network is the set of all injections satisfying the conservation of energy. If the network has a tree topology, we show that the injection region with voltage magnitude, line loss constraints, line flow constraints and certain bus power constraints has the same set of Pareto optimal points as its convex hull. The set of Pareto-optimal points are of interest since these are the the optimal solutions to the minimization of a increasing convex function over the injection region. For non-tree networks, we obtain a weaker result by characterize the convex hull of the voltage constraint injection region for lossless cycles, a lossless cycle with a chord and certain combinations of these networks. The convex hull is of interest since they correspond to optimizing linear functions.
Defining characteristics of Tn5 Transposase non-specific DNA binding (2006)

While non-specific DNA plays a role in target localization for many recombinases, transcription factors and restriction enzymes, the importance of non-specific DNA interactions for transposases has not been investigated. Here, we discuss non-specific DNA-Tn5 Transposase (Tnp) interactions and suggest how they stabilize the Tnp and modulate Tnp localization of the 19 bp Tnp recognition end sequences (ESes). DNA protection assays indicate that full-length Tnp interacts efficiently with supercoiled DNA that does not contain ESes. These interactions significantly prolong the lifetime of Tnp, in vitro. The balance between non-specific DNA bound and free Tnp is affected by DNA topology, yet, intermolecular transfer of active Tnp occurs with both supercoiled and linear non-specific DNA. Experiments with substrates of varying lengths show that Tn5 Tnp can utilize non-specific DNA to facilitate localization of an intramolecular ES over distances less than 464 bp. Finally, synaptic complex formation is inhibited in the presence of increasing concentrations of supercoiled and linear pUC19. These experiments strongly suggest that Tn5 Tnp has a robust non-specific DNA binding activity, that non-specific DNA modulates ES sequence localization within the global DNA, most likely through a direct transfer mechanism, and that non-specific DNA binding may play a role in the cis bias manifested by Tn5 transposition.
Formation of stable monolayer membranes and related structures in dilute aqueous solution from two-headed ammonium amphiphiles (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of stable monolayer membranes and related structures in dilute aqueous solution from two-headed ammonium amphiphilesYoshio Okahata and Toyoki KunitakeCite this: J. Am. Chem. Soc. 1979, 101, 18, 5231–5234Publication Date (Print):August 1, 1979Publication History Published online1 May 2002Published inissue 1 August 1979https://pubs.acs.org/doi/10.1021/ja00512a020https://doi.org/10.1021/ja00512a020research-articleACS PublicationsRequest reuse permissionsArticle Views424Altmetric-Citations131LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Anti-nerve growth factor Ab abrogates macrophage-mediated HIV-1 infection and depletion of CD4+T lymphocytes in hu-SCID mice (2003)

Infection by HIV-1 causes persistent, long-term high virus production in macrophages. Major evidence, both in humans and in primate models, shows the crucial role of macrophages in sustaining virus production and in mediating a cytopathic effect on bystander CD4+ T lymphocytes and neuronal cells. In the present study, we used severe combined immunodeficient (SCID) mice engrafted with human peripheral blood lymphocytes (hu-PBL-SCID mice) to investigate the in vivo effect of HIV-1-infected macrophages on virus spread and CD4+ T lymphocyte depletion, and the ability of a mAb against nerve growth factor (NGF, a neurokine essential for the survival of HIV-1-infected macrophages) to suppress the pathogenetic events mediated by infected macrophages. Injection of mice with as few as 500 HIV-exposed macrophages causes (i) complete depletion of several millions of autologous CD4+ T lymphocytes, (ii) sustained HIV viremia, and (iii) spreading of HIV-1 DNA in mouse lymphoid organs. In contrast, in vivo treatment with an anti-NGF Ab completely abrogates all effects mediated by HIV-infected macrophages. Taken together, the results demonstrate the remarkable power of macrophages in sustaining in vivo HIV-1 infection, and that such a phenomenon can be specifically abrogated by an anti-NGF Ab. This may open new perspectives of experimental approaches aimed at selectively eliminating persistently infected macrophages from the bodies of HIV-infected patients.
Minimal radial basis function neural networks for nonlinear channel equalisation (2000)

The results of linear and nonlinear channel equalisation in data communications are presented, using a recently developed minimal radial basis function neural network structure, referred to as the minimal resource allocation network (MRAN). The MRAN algorithm uses online learning, and has the capability to grow and prune the RBF network&apos;s hidden neurons ensuring a parsimonious network structure. Compared to earlier methods, the proposed scheme does not have to estimate the channel order first, and fix the model parameters. Results showing the superior performance of the MRAN algorithm for two linear channels (minimum and non-minimum phase) for 2PAM signalling, and three nonlinear channels for 2PAM and 4QAM signalling, are presented.
Effects of supplemental chromium on immune responses of periparturient and early lactation dairy cows1 (1993)

The objective of the present study was to determine the effects of supplemental dietary chromium on immune responses of dairy cows subjected to physical and metabolic stresses associated with late pregnancy, calving, early lactation, and peak milk yield. Nine periparturient dairy cows were supplemented with chelated Cr (.5 ppm/d) from 6 wk prepartum (wk -6) through 16 wk postpartum (wk 16), and 10 cows were unsupplemented controls. To assess humoral immune responses, all cows were immunized with ovalbumin (OVA; s.c.) and human erythrocytes (HRBC; i.v.) on wk -2 and 2, and sera from weekly blood samples were assayed for content of antigen-specific antibody. Cell-mediated immunity was assessed in vitro using antigen (OVA)- and mitogen-stimulated peripheral blood mononuclear cell (PBMC) blastogenesis of cells collected biweekly from wk -2 and 6. Supplemental Cr caused anti-OVA antibody responses (P < .01) and mitogen-stimulated blastogenic responses of PBMC (P = .05) to be elevated, was associated with lowered OVA-stimulated blastogenic responses of PBMC (P < .01), and had no overall effect on antibody responses to HRBC (P > .10) relative to responses of control cows. These results confirmed and extended our previous observations that supplemental Cr can alter specific immune responses of stressed cattle.
“Desolated and Deprived”: a CDA Approach to Liu’s Discourse (2010)

Language and society are closely interrelated as every change in the society is reflected in our language. Critical discourse analysis (CDA) is an important tool for our understanding of the society. We made a CDA analysis on the announcements Liu made in her prosecution against Li, a famous anchor in China, following a sociological investigation approach proposed by Fairclough in his Language and Power, and cognitive method as a supplement, trying to find out what is buried under the language. Key words: CDA, social ethics, narrative, ideology, profit-loss scheme Resume: Il existe une relation inseparable entre la langue et la societe et chaque changement social se reflete dans la langue. En tant qu’un outil puissant d’analyse, l’analyse critique du discours joue un role tres important dans la connaissance de la societe. Nons effectuons, en employant la methode d’analyse sociale preconisee par Fairclough et la methode d’analyse cognitive, l’analyse critique du discours du proces de Liu intente contre Li. Avec le corpus de quatres declarations de Liu Yin, nons s’efforcons d’explorer la conscience cachee sous la langue superficielle. Mots-cles: analyse critique du discours, morale sociale, narration, ideologie sociale, logique de benefice et de perte 摘要：語言與社會之間具有密不可分的關係，社會的每一個變化都能在語言中得到體現。批評話語分析作為一項有力的分析工具對於認識社會具有非常重要的作用。我們使用 Fairclough所宣導的社會分析方法結合認知分析方法對劉某訴李某案進行了批評話語分析，語料為劉穎所作的四次公開聲明，盡力挖掘其表面語言背後所隱藏的意識。 關鍵詞：批評話語分析；社會道德；敍事；社會意識形態；賺賠邏輯
Microdevice arrays of high aspect ratio poly(dimethylsiloxane) pillars for the investigation of multicellular tumour spheroid mechanical properties (2014)

We report the design, fabrication and evaluation of an array of microdevices composed of high aspect ratio PDMS pillars, dedicated to the study of tumour spheroid mechanical properties. The principle of the microdevice is to confine a spheroid within a circle of micropillars acting as peripheral flexible force sensors. We present a technological process for fabricating high aspect ratio micropillars (300 μm high) with tunable feature dimensions (diameter and spacing) enabling production of flexible PDMS pillars with a height comparable to spheroid sizes. This represents an upscale of 10 along the vertical direction in comparison to more conventional PDMS pillar force sensors devoted to single cell studies, while maintaining their force sensitivity in the same order of magnitude. We present a method for keeping these very high aspect ratio PDMS pillars stable and straight in liquid solution. We demonstrate that microfabricated devices are biocompatible and adapted to long-term spheroid growth. Finally, we show that the spheroid interaction with the micropillars' surface is dependent on PDMS cellular adhesiveness. Time-lapse recordings of growth-induced micropillars' bending coupled with a software program to automatically detect and analyse micropillar displacements are presented. The use of these microdevices as force microsensors opens new prospects in the fields of tissue mechanics and pharmacological drug screening.
Ligand Binding and Phagocytosis by CD16 (Fc γ Receptor III) Isoforms (1995)

CD16, the low affinity Fc gamma receptor III for IgG (Fc gamma RIII), exists as a polypeptide-anchored form (Fc gamma RIIIA or CD16A) in human natural killer cells and macrophages and as a glycosylphosphatidylinositol-anchored form (Fc gamma RIIIB or CD16B) in neutrophils. CD16A requires association of the gamma subunit of Fc epsilon RI or the zeta subunit of the TCR-CD3 complex for cell surface expression. The CD16B is polymorphic and the two alleles are termed NA1 and NA2. In this study, CD16A and the two alleles of CD16B have been expressed in Chinese hamster ovary (CHO) cells and their ligand binding and phagocytic properties analyzed. The two allelic forms of CD16B showed a similar affinity toward human IgG1. However, the NA1 allele showed approximately 2-fold higher affinity for the IgG3 than the NA2 allele. Although all three forms of CD16 efficiently bound rabbit IgG-coated erythrocytes (EA), only CD16A coexpressed with the gamma subunit phagocytosed EA. The phagocytosis mediated by CD16A expressed on CHO cells was independent of divalent cations but dependent on intact microfilaments. CHO cells expressing CD16A-gamma and CD16A-zeta chimeras also phagocytosed EA. The phagocytosis was specifically inhibited by tyrphostin-23, a tyrosine kinase inhibitor. In summary, our results demonstrate that glycosylphosphatidylinositol-anchored CD16B alleles differ from CD16A in their ability to mediate phagocytosis. Furthermore, since studies with other Fc gamma Rs have shown that CHO cells lack the phagocytic pathway mediated by the cytoplasmic domain of Fc gamma Rs, the phagocytosis of EA by CHO cells stably transfected with CD16A and CD16A-subunit chimera provides an ideal system to dissect the phagocytic signaling pathways mediated by these Fc gamma R-associated subunits.
Applications of Chitin and Chitosan for Biomaterials (1996)

(1996). Applications of Chitin and Chitosan for Biomaterials. Biotechnology and Genetic Engineering Reviews: Vol. 13, No. 1, pp. 383-420.
Employing oxygen-plasma posttreatment to improve the laser-induced damage threshold of ZrO_2 films prepared by the electron-beam evaporation method (2004)

ZrO2 films are deposited by the electron-beam evaporation method. Parts of the prepared samples are post-treated with oxygen plasma at the environment temperature. The laser-induced damage threshold (LIDT) of the films increases from 15.9 to 23.1 J/cm2 after treatment with oxygen plasma. Compared with that of the as-grown samples, significant reduction of the average microdefect density and absorption are found after oxygen-plasma posttreatment. These results indicate that the oxygen-plasma posttreatment technique is an effective and simple method for reducing the microdefect density and absorption to improve the LIDT.