gebhart/patrepeval huggingface dataset:
PP.14.17 (2015)

Objective: To investigate whether tetrahydrobiopterin (BH4) combination with Nebivolol recouple eNOS and reverse or prevent cardiac hypertrophy, fibrosis, and diastolic dysfunction in spontaneous hypertensive rats (SHR). Design and method: 12 weeks, 60 SHR were purchased from Vital River Laboratories in Beijing and were randomized to10 groups (n = 6 for each). All SHR were acclimatized to a new situation for a week and receive low-dose (87.5 mg/kg.d) or high-dose(175 mg/kg.d) BH4, or combination with low dose nebivolo (5 mg/kg.d) and high dose(10 mg/kg.d) and placebo for one week. Cardiac function was measured by arious conventional and tissue Doppler echocardiographic index transthoracic check and invasive conductance catheter analysis before and after treatment. RT-PCR, western-blot and immunohistochemistry were used to detect the levels of myocardium BH4, superoxide, eNOS, phospholamban (PLB), protein kinase G(PKG), sarcoplasmic reticulumCa2+ATPase(SERCA 2a)expression of mRNA and their protein expression in myocardium. We also measured Brain Natriuretic Peptid (BNP) and nitrite (an NO storage molecule). α Results: The results showed that cardiac diastolic function of SHR in vehicle(VEH) group trend worsen than that of baseline. After feeding high dose or low dose BH4 the index of diastolic function,such as tau,min dp/dt,left ventricular end of diastolic pressure(LVEDP) reversed especially low dose BH4,but P = NS. High dose nebivolol didn’t reverse the LVEDP increase, even showed mildly improvement compared with VEH. However, low dose nebivolol reversed the increasing LVEDP (12.318 ± 3.177 vs 18.1295 ± 2.0673,P = 0.029). The mRNA level of eNOS increase mildly after separately feeding nebivolol or BH4,while cosupplementation BH4 and nebivolol the mRNA of eNOS increased significantly. The protein expression of eNOS in myocardium suggested the same trend. This finding indicated that low dose BH4 (17.5 mg/d) combination low dose nebivolol (1.25 mg/d) were better than higher doses of them for expression of mRNA and protein in eNOS, especially in the enhance aspect of diastolic function. ± ± Conclusions: Oral BH4 cosupplementation nebivolol for one week improves the cardiac diastolic function in SHR by activation eNOS, augment NO production and its bioactivity. They act synergistically to improve the diastolic function after combination them with low doses.
Molecular and Biochemical Analysis of Two β-Galactosidases fromBifidobacterium infantisHL96 (2001)

ABSTRACT Two genes encoding β-galactosidase isoenzymes, β-galI and β-galIII , from Bifidobacterium infantis HL96 were revealed on 3.6- and 2.4-kb DNA fragments, respectively, by nucleotide sequence analysis of the two fragments. β-galI (3,069 bp) encodes a 1,022-amino-acid (aa) polypeptide with a predicted molecular mass of 113 kDa. A putative ribosome binding site and a promoter sequence were recognized at the 5′ flanking region of β-galI . Further upstream a partial sequence of an open reading frame revealed a putative lactose permease gene transcribing divergently from β-galI . The β-galIII gene (2,076 bp) encodes a 691-aa polypeptide with a calculated molecular mass of 76 kDa. A rho-independent transcription terminator-like sequence was found 25 bp downstream of the termination codon. The amino acid sequences of β-GalI and β-GalIII are homologous to those found in the LacZ and the LacG families, respectively. The acid-base, nucleophilic, and substrate recognition sites conserved in the LacZ family were found in β-GalI, and a possible acid-base site proposed for the LacG family was located in β-GalIII, which featured a glutamate at residue 160. The coding regions of the β-galI and β-galIII genes were each cloned downstream of a T7 promoter for overexpression in Escherichia coli . The molecular masses of the overexpressed proteins, as estimated by polyacrylamide gel electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, agree with their predicted molecular weights. β-GalI and β-GalIII were specific for β- d -anomer-linked galactoside substrates. Both are more active in response to ONPG ( o -nitrophenyl-β- d -galactopyranoside) than in response to lactose, particularly β-GalIII. The galacto-oligosaccharide yield in the reaction catalyzed by β-GalI at 37°C in 20% (wt/vol) lactose solution was 130 mg/ml, which is more than six times higher than the maximum yield obtained with β-GalIII. The structure of the major trisaccharide produced by β-GalI catalysis was characterized as O -β- d -galactopyranosyl-(1-3)- O -β- d -galactopyranosyl-(1-4)- d -glucopyranose (3′-galactosyl-lactose).
Prognostic significance of p27 Kip1 expression in bladder cancer (2007)

Low expression of p27(Kip1) was a significant independent predictor of pelvic recurrence, progression to metastasis, death from disease and death from any cause, in patients with bladder cancer.
Transmissible spongiform encephalopathies (TSE): minimizing the risk of transmission by biological/biopharmaceutical products: an industry perspective. (1996)

Several guidelines and recommendations have been published on assessing the potential risk of a biological product being contaminated with an agent causing a Transmissible Spongiform Encephalopathy (TSE). Basic principles which can be used during the manufacturing of biological products to minimize the risk of transmission of TSE agents include the following: (i) obtaining animals, tissues or animal-derived raw materials from countries in which the relevant TSE agent is reported to be absent; (ii) screening animal-derived material for appropriate animal husbandry, feeding practices, health certification, tissue procurement and processing practices, and human-derived material for appropriate medical history information and, if relevant, plasma donor exclusion criteria; (iii) selection of tissue source of raw material with reference to potential risk for harbouring or amplifying TSE agents; (IV) human tropism of TSE agent of concern; (v) quantity of raw material used to manufacture a dose of product; (vi) number of daily doses required for indication; (vii) route of administration; (viii) indication and age of patient; and (ix) clearance capabilities by the product purification scheme. A risk evaluation will be represented for Trasylol (Aprotinin) which is manufactured from bovine lung tissue. Trasylol is a protease inhibitor used in the control of blood loss during open heart surgery. Bovine Spongiform Encephalopathy (BSE) is a concern when a product is derived from a bovine source. Even though only bovine lung tissue from BSE-free countries is used to manufacture Trasylol, it was decided, based on the primary tissue source selection, to evaluate the potential of the purification process to clear a TSE agent. Duplicate "spiking experiments" were performed using a mouse-adapted strain of scrapie as the model for BSE. Four steps in the purification process were evaluated. Titration of "spiked" loading material and output samples was performed at dilutions of 10(-4) to 10(-7) and undiluted to 10(-7), respectively. The sum of the purification steps evaluated provided a clearance factor in excess of 18 log10 for a TSE agent. An overall safety factor of 30 is obtained using the notification document issued by the Federal Health Office (Germany), dated February 16, 1994, on "Safety Requirements for Medical Products Derived from Body Constituents of Cattle, Sheep, or Goats to Avoid Risk of Transmission of BSE or Scrapie". Based on a six-parameter risk assessment, this document considered a product of low-risk for transmitting TSE agents if obtaining or exceeding a score of 20. Principles for minimizing the risk of transmission of TSE agents in products derived from primary tissues are contrasted to strategies for biotechnology products.
Representations for Rigid Solids: Theory, Methods, and Systems (1980)

Computer-based systems for modehng the geometry of rigid solid objects are becoming increasingly important in mechanical and civil engineering, architecture, computer graphics, computer vision, and other fields that deal with spatial phenomena. At the heart of such systems are symbol structures (representations) designating "abstract solids" (subsets of Euclidean space) that model physical solids. Representations are the sources of data for procedures which compute useful properties of objects.
<title>Translation, rotation, and scaling invariant object and texture classification using polyspectra</title> (1990)

The problem addressed in this paper is the detection and classification of deterministic objects and random textures in a noisy scene. An energy detector is developed in the cumulant domain, by exploiting the noise insensitivity of higher-order statistics. An efficient implementation of this detector is described, using matched filtering. Its performance is analyzed using asymptotic distributions in a binary hypothesis testing framework. Object and texture classifiers are derived using higher-order statistics. They are minimum distance classifiers in the cumulant domain, and can be efficiently implemented using a bank of matched filters. Further, they are robust to additive Gaussian noise and insensitive to object shifts. Extensions, which can handle object rotation and scaling are also discussed. An alternate texture classifier is derived from an ML viewpoint, that is more efficient at the expense of complexity. The application of these algorithms to texture modeling is shown and consistent parameter estimators are obtained. Simulations are shown for both the object and the texture classification problems.
The Design of a LiFePO4/Carbon Nanocomposite With a Core–Shell Structure and Its Synthesis by an In Situ Polymerization Restriction Method (2008)

Truly long-life: A LiFePO4/carbon composite containing a highly crystalline LiFePO4 core with a size of about 20–40 nm and a semi-graphitic carbon shell with a thickness of about 1–2 nm provides both high power and very long cycling life (see picture). The synthetic method can be extended to the preparation of other materials, such as Li4Ti5O12/carbon and Mn3O4/carbon composites. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z802539_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Purification of the glucocorticoid receptor from rat liver cytosol. (1979)

The [3H]-triamcinolone acetonide-labeled glucocorticoid receptor from rat liver cytosol was purified to 85% homogeneity according to sodium dodecyl sulfate gel electrophoresis. It consisted of one subunit with a molecular weight of 89,000 and had one ligand-binding site per molecule. The purification involved sequential chromatography on phosphocellulose, DNA-cellulose twice, and Sephadex G-200. Between the two chromatography steps on DNA-cellulose, the receptor was heat activated. The receptor was affinity eluted from the second DNA-cellulose column with pyrodixal 5'-phosphate. The purification achieved in the first three chromatographic steps varied between 60 and 95% homogeneity in different experiments. After chromatography on the second DNA-cellulose column, the steroid.receptor complex had a Stokes radius of 6.0 nm and a sedimentation coefficient of 3.4 S in 0.15 M KCl. In the absence of KCl, the sedimentation coefficient was 3.6 S. After concentration on hydroxylapatite, the steroid.receptor complex was analyzed by isoelectric focusing in polyacrylamide gel. The radioactivity was shown to focus together with the major protein band with pI 5.8. Following limited proteolysis with trypsin, the radioactivity, together with the major protein band, focused at pI 6.2 as previously described for the unpurified steroid.receptor complex.
Multi-centre investigation on reference ranges for ROTEM thromboelastometry (2005)

Reagent-supported thromboelastometry (TEM) with the ROTEM Whole Blood Haemostasis Analyser is an enhancement of thromboelastography, a method that is increasingly used for the point of care monitoring of acute perioperative bleeding disorders. We investigated the reference ranges of two activated tests (INTEM and EXTEM) and a test analysing specifically the fibrin component of coagulation (FIBTEM) in a multi-centre approach. The reference ranges obtained for the clotting time (CT), clot formation time (CFT), alpha angle (ALP), maximum clot firmness (MCF) and clot lysis parameters were comparable from centre to centre. INTEM: CT equals; 137-246 s, CFT equals; 40-100 s, MCF equals; 52-72 mm. EXTEM: CT equals; 42-74 s, CFT equals; 46-148 s, MCF equals; 49-71 mm. FIBTEM: MCF equals; 9-25 mm. ROTEM whole blood coagulation correlated weakly with a trend towards enhanced coagulation in females compared with males and in advanced age. The repeatability (within-run imprecision) of the results was dependent on the test with the following coefficients of variation: 1-5% (clot firmness, alpha angle), 3-12% (CT, CFT), 6-13% (FIBTEM clot firmness). Citrated blood samples were stable for ROTEM analysis stored within 6 h from drawing. In summary, the data showed that ROTEM thromboelastometry yields consistent values between centres and that providing general orientating reference ranges seems to be possible.
Use of peptide aldehydes to generate transition-state analogs of elastase (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of peptide aldehydes to generate transition-state analogs of elastaseRobert C. ThompsonCite this: Biochemistry 1973, 12, 1, 47–51Publication Date (Print):January 1, 1973Publication History Published online1 May 2002Published inissue 1 January 1973https://pubs.acs.org/doi/10.1021/bi00725a009https://doi.org/10.1021/bi00725a009research-articleACS PublicationsRequest reuse permissionsArticle Views321Altmetric-Citations120LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Matrix-assisted laser desorption/ionization mass spectrometry of DNA using photocleavable biotin (1999)

Oligonucleotides containing a photocleavable biotin (5'-PC-biotin) were analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) with wavelengths in the ultraviolet (UV) and infrared (IR) from solution and after capture on streptavidin-coated agarose or magnetic beads. The analysis was used to monitor the release of the oligonucleotides as a result of photochemical cleavage of the biotinylated linker. Near-UV pulses (UV-MALDI) led to predominant release of the photocleaved product. In contrast, only the uncleaved analyte was detected using IR pulses (IR-MALDI). Results from MALDI analysis are also presented for DNA containing a photocleavable 5'-amino group which can be covalently linked to a variety of activated surfaces and marker molecules. In a demonstration of this approach, a 5'-PC-biotinylated 49 nt RNA oligonucleotide was enzymatically synthesized using a PC-biotin-r(AG) dinucleotide primer, captured on streptavidin coated magnetic beads and analyzed by UV-MALDI. Potential applications of photocleavable linkers combined with MALDI for the analysis of nucleic acids are discussed.
Accelerating enterprise solid-state disks with non-volatile merge caching (2010)

Flash memory is now widely used in the design of solid-state disks (SSDs) as they are able to sustain significantly higher I/O rates than even high-performance hard disks, while using significantly less power. These characteristics make SSDs especially attractive for use in enterprise storage systems, and it is predicted that the use of SSDs will save 58,000 MWh/year by 2013. However, Flash-based SSDs are unable to reach peak performance on common enterprise data patterns such as log-file and metadata updates due to slow write speeds (an order-of-magnitude slower than reads) and the inability to do in-place updates. In this paper, we utilize an auxiliary, byte-addressable, non-volatile memory to design a general purpose merge cache that significantly improves write performance. We also utilize simple read policies that further improve the performance of the SSD without adding significant overhead. Together, these policies reduce the average response time by more than 75%, making it possible to meet performance requirements with fewer drives.
Causal factors and symptoms of human behavior error domain in human-foftware interaction (1992)

This study is to define a congitive paradigm including a new model of common cause human behavior error domain and to analyze their causal factors and their properties of common cause human error characteristics in software engineering. A laboratory study was performed to analyze the common causes of human behavior domain error in software development and to identify software design factors contributing to the common cause effects in common cause failure redundancy. The results and analytical paradigm developed in this research can be applied to reliabbility improvement and cost reduction in software development for many applications. Results are also expected to provide training guidelines for software engineers and for more effective design of ultra-high reliable software packages.
Expression of epidermal-growth-factor receptor in the K562 cell line by transfection. Altered receptor biochemistry (1990)

The epidermal-growth-factor (EGF) receptor was expressed in the human erythroleukaemic cell line K562 by transfection of the receptor cDNA. EGF-receptor biochemistry appears altered in the K562 transfectants. Autophosphorylation of the K562 receptor is not stimulated substantially by EGF. Tyrosine kinase activity of the receptor is high in the absence of EGF, whereas receptor affinity for EGF is low. K562 cells are shown to lack mRNA for transforming growth factor alpha (TGF alpha). Therefore autocrine stimulation of the K562 receptor, at least by TGF alpha, does not explain the observed receptor biochemistry. The K562 receptor is phosphorylated at a single major site in intact cells, a threonine residue that may be Thr-669. Possible mechanisms of regulation of the EGF receptor in the K562 transfectants are discussed.
Study of the interaction between salivary proline‐rich proteins and a polyphenol by 1H‐NMR spectroscopy (1994)

The interaction between salivary proline-rich proteins and plant polyphenols (tannins) in the oral cavity and their subsequent precipitation influences the taste, texture and nutritional value of food; it is thought to be responsible for the astringency of many foods and beverages. To investigate the interaction, two-dimensional 1H-NMR studies have been carried out on the binding of a representative polyphenol, pentagalloyl glucose, to two synthetic peptides (19 and 22 residues in length) that are typical of the repeat sequence of mouse salivary proline-rich protein MP5. Intermolecular nuclear Overhauser effects and chemical shift changes show that the main binding sites on the peptides are proline residues together with the preceding amide bond and amino acid. The interaction is principally a hydrophobic association between a galloyl ring and the pyrrolidine ring face containing the C alpha proton, but secondary hydrogen-bonding effects help to stabilise the complex. Very similar interactions are seen for both peptides. The conformation of the peptides remains extended on binding. The chemical shift changes seen for many of the peptide protons can be fitted to a simple binding curve with dissociation constant of around 40 mM, but some protons show evidence of cooperative binding involving several galloyl groups. Higher concentrations of pentagalloyl glucose lead to a reduced off-rate from the complex and eventual precipitation which implies that precipitation is caused by a kinetic competition between aggregation and dissociation of the complex.
Identification and characterization of a novel gene, c1orf109, encoding a CK2 substrate that is involved in cancer cell proliferation (2012)

Taken together, our findings suggest that C1ORF109 may be the downstream target of protein kinase CK2 and involved in the regulation of cancer cell proliferation.
Identification of Novel Allosteric Regulators of Human‐Erythrocyte Pyruvate Kinase (2007)

Erythrocyte pyruvate kinase (PK) is an important glycolytic enzyme, and manipulation of its regulatory behavior by allosteric modifiers is of interest for medicinal purposes. Human-erythrocyte PK was expressed in Rosetta cells and purified on an Ni-NTA column. A search of the small-molecules database of the National Cancer Institute (NCI), using the UNITY software, led to the identification of several compounds with similar pharmacophores as fructose-1,6-bisphosphate (FBP), the natural allosteric activator of the human kinases. The compounds were subsequently docked into the FBP binding site using the programs FlexX and GOLD, and their interactions with the protein were analyzed with the energy-scoring function of HINT. Seven promising candidates, compounds 1-7, were obtained from the NCI, and subjected to kinetics analysis, which revealed both activators and inhibitors of the R-isozyme of PK (R-PK). The allosteric effectors discovered in this study could prove to be lead compounds for developing medications for the treatment of hemolytic anemia, sickle-cell anemia, hypoxia-related diseases, and other disorders arising from erythrocyte PK malfunction.
Information system integration (2000)

article Free Access Share on Information system integration Author: Wilhelm Hasselbring Tilburg Univ., Tilburg, The Netherlands Tilburg Univ., Tilburg, The NetherlandsView Profile Authors Info & Claims Communications of the ACMVolume 43Issue 6June 2000pp 32–38https://doi.org/10.1145/336460.336472Published:01 June 2000Publication History 237citation16,262DownloadsMetricsTotal Citations237Total Downloads16,262Last 12 Months1,549Last 6 weeks174 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteView all FormatsPDF