gebhart/patrepeval huggingface dataset:
Ferulic Acid Inhibits 7,12-Dimethylbenz[ α ]anthracene-Induced Hamster Buccal Pouch Carcinogenesis (2008)

The aim of this study was to assess the chemopreventive efficacy of ferulic acid in 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. We induced oral squamous cell carcinoma in the buccal pouch of male Syrian golden hamsters by painting with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. The tumor incidence, tumor volume, and tumor burden that were formed in the hamster buccal pouch were determined. The activities of carcinogen detoxification agents and status of lipid peroxidation and antioxidants were also estimated by specific colorimetric methods. We observed 100% tumor formation in DMBA-painted animals. The status of carcinogen-detoxifying agents, lipid peroxidation, and antioxidants was significantly disrupted in DMBA-painted animals. Oral administration of ferulic acid at a dose of 40 mg/kg of body weight to DMBA-painted animals on days alternate to DMBA painting for 14 weeks significantly prevented the tumor incidence, tumor volume, and tumor burden. Ferulic acid exhibited potent anti-lipid peroxidative effects as well as the ability to modulate the status of carcinogen-detoxifying agents and antioxidants in DMBA-painted animals. Our results demonstrate that ferulic acid has potent chemopreventive and antioxidant functions in DMBA-induced hamster buccal pouch carcinogenesis.
Structure-Activity Relationship of Nerve-Highlighting Fluorophores (2013)

Nerve damage is a major morbidity associated with numerous surgical interventions. Yet, nerve visualization continues to challenge even the most experienced surgeons. A nerve-specific fluorescent contrast agent, especially one with near-infrared (NIR) absorption and emission, would be of immediate benefit to patients and surgeons. Currently, there are only three classes of small molecule organic fluorophores that penetrate the blood nerve barrier and bind to nerve tissue when administered systemically. Of these three classes, the distyrylbenzenes (DSBs) are particularly attractive for further study. Although not presently in the NIR range, DSB fluorophores highlight all nerve tissue in mice, rats, and pigs after intravenous administration. The purpose of the current study was to define the pharmacophore responsible for nerve-specific uptake and retention, which would enable future molecules to be optimized for NIR optical properties. Structural analogs of the DSB class of small molecules were synthesized using combinatorial solid phase synthesis and commercially available building blocks, which yielded more than 200 unique DSB fluorophores. The nerve-specific properties of all DSB analogs were quantified using an ex vivo nerve-specific fluorescence assay on pig and human sciatic nerve. Results were used to perform quantitative structure-activity relationship (QSAR) modeling and to define the nerve-specific pharmacophore. All DSB analogs with positive ex vivo fluorescence were tested for in vivo nerve specificity in mice to assess the effect of biodistribution and clearance on nerve fluorescence signal. Two new DSB fluorophores with the highest nerve to muscle ratio were tested in pigs to confirm scalability.
Pre-Steady-State Study of Recombinant Sesquiterpene Cyclases (1997)

An Escherichia coli expression system was used to generate hexahistidyl-tagged plant sesquiterpene cyclases, which were readily purified by a single affinity chromatographic step. Genes for Hyoscyamus muticus vetispiradiene synthase (HVS), a chimeric 5-epi-aristolochene synthase (CH3), and a chimeric sesquiterpene cyclase possessing multifunctional epi-aristolochene and vetispiradiene activity (CH4) were expressed in bacterial cells, which resulted in the sesquiterpene cyclases accumulating to 50% of the total protein and 35% of the soluble protein. From initial velocity experiments, the Michaelis constant for HVS was 3.5 microM, while CH3 and CH4 exhibited smaller values of 0.7 and 0.4 microM, respectively. Steady-state catalytic constants were from 0.02 to 0.04 s-1. A combination of pre-steady-state rapid quench experiments, isotope trapping experiments, and experiments to measure the burst rate constant as a function of substrate concentration revealed that turnover in all three cyclases is limited by a step after the initial chemical step involving rupture of the carbon-oxygen bond in farnesyl diphosphate (FPP). Rate constants for the limiting step were 10-70-fold smaller than for the initial chemical step. Dissociation constants for the enzyme-substrate complex (20-70 microM) were determined from the pre-steady-state experiments and were significantly larger than the observed Michaelis constants. A mechanism that involves an initial, rapid equilibration of enzyme with substrate to form an enzyme-substrate complex, followed by a slower conversion of FPP to an enzyme-bound hydrocarbon and a subsequent rate-limiting step, is proposed for the three enzymes. Interestingly, the multifunctional chimeric enzyme CH4 exhibited both a tighter binding of FPP and a faster conversion of FPP to products than either of its wild-type parents.
Ligation of CD137 receptor prevents and reverses established anergy of CD8+ cytolytic T lymphocytes in vivo (2003)

T-cell anergy is a tolerance mechanism defined as a hyporesponsive status of antigen-specific T cells upon prior antigen encounter and is believed to play a critical role in the evasion of tumor immunity and the amelioration of allogeneic transplant rejection. Molecular mechanisms in controlling T-cell anergy are less known. We show here that administration of an agonistic monoclonal antibody (mAb) to CD137, a member of the tumor necrosis factor receptor superfamily, prevents the induction of CD8+ cytolytic T-lymphocyte (CTL) anergy by soluble antigens. More importantly, CD137 mAb restores the functions of established anergic CTLs upon reencountering their cognate antigen. As a result, infusion of CD137 mAb inhibits progressive tumor growth that is caused by soluble tumor antigen-induced tolerance in a P815R model. CD137 mAb also restores proliferation and effector functions of anergic alloreactive 2C T cells in a bone marrow transplantation model. Our results indicate that ligation of CD137 receptor delivers a regulatory signal for T-cell anergy and implicate manipulation of the CD137 pathway as a new approach to break T-cell tolerance.
Fish lesions in the Chesapeake Bay: Pfiesteria-like dinoflagellates and other etiologies. (1998)

Ulcerative lesions and mass mortalities of Atlantic estuarine fish, particularly menhaden (Brevoortia tyrannus), have been associated with exposure to Pfiesteria-like dinoflagellates and their toxins. We collected fish from the Chicamacomico River, Maryland, and observed solitary ulcerative lesions on the majority of menhaden sampled. One striped bass (Morone saxatilis) had an area of reddening around the base of the dorsal fin. Bluegill (Lepomis machrochirus), channel catfish (Ictalurus punctatus), yellow perch (Perca flavescens), and carp (Cyprinus carpio) were externally nonremarkable. Histologically ulcerative menhaden lesions demonstrated marked chronic inflammatory infiltrate in large areas of exposed necrotic muscle. The ulcers contained granulomata with fungal hyphae in the necrotic tissue. Gram negative rod-shaped bacteria were also observed in the lesions, a common finding in ulcers of aquatic organisms. Our data suggest that typical ulcerative lesions observed on fish from areas of Pfiesteria-like dinoflagellate blooms are reflective of dermatosis, which may be related to a variety of individual or combined environmental stressors. Exposure to dinoflagellate toxin)s) potentially represents one such stressor. The role of Pfiesteria-like dinoflagellate toxin in fish primary lesion development is currently under investigation.
Algorithm Based on Heuristic Strategy to Infer Lossy Links in Wireless Sensor Networks (2014)

With the maturing of the actual application of wireless sensor networks, network fault management is eagerly demanded. Severe link packet loss affects the performance of wireless sensor networks, so it must be found and repaired. Subject to the constraints on limited resources, lossy link is inferred using end to end measurement and network tomography. The algorithm based on heuristic strategy is proposed. This maps the problem of lossy links inferences to minimal set-cover problems. The performance of inference algorithms is evaluated by simulation, and the simulation results indicate feasibility and efficiency of the method.
Review : High-pressure, microbial inactivation and food preservation (1995)

High pressure (1 to 10 kbars, i.e. 100-1000 MPa) affects biological constituents and systems. Several physicochemical properties of water are modified, such as the density, the ionic dissociation (and pH), and the melting point of ice. Pressure-induced unfolding, aggregation, and gelation of food proteins mainly depend on the effects of pressure on various noncovalent bonds and interactions. Enzyme inactivation (e.g., of ATPases) also results from similar effects, but some enzymes, including oxidative enzymes from fruits and vegetables, are strongly baroresistant. Chemical reactions, macromolecular transconformations, changes in membrane structure, or changes in crystal form and melting point that are accompanied by a decrease in volume are enhanced under pressure (and vice versa). Several of these phenomena, still poorly identified, are involved in the high inactivation ratio (5–6 logarithmic cycles) of most vegetative microbial cells: gram-negative bacteria, yeasts, complex viruses, molds, and gram-positive bacteria, in this decreasing order of sensitivity to pressure. Much variability is noted in the baroresistance of microorganisms, even within one single species or genus. Other parameters influence this resistance: pressure level, holding time (a two-phase kinetics of inactivation is often observed that prevents the calculation of decimal reduction times), temperature of pressure processing (temperatures above 50°C or between –30 and +5°C enhancing inactivation), composition of the medium or of the food (the pH having apparently little influence, but high salt or sugar concentrations, and low water contents, exerting very strong baroprotective effects). Taking into account the baroprotective effects of some food constituents and the strong resistance of some microbial strains, recent research aims at combined processes in which high pressure is associated with moderate temperature, CO 2 , other bacteriostatic agents, or to nonthermal physical processes such as ultrasounds, alternative currents, high-voltage electric pulses, and so forth. The safety and refrigerated shelf life of pressurized foods could be maintained or extended, while the sensorial quality should improve due to the reduced severity of thermal processing. Further research is, however, needed for the regulatory authorities to assess and accept these novel foods and processes.
Humanized large-scale expanded endothelial colony–forming cells function in vitro and in vivo (2009)

Endothelial progenitor cells are critically involved in essential biologic processes, such as vascular homeostasis, regeneration, and tumor angiogenesis. Endothelial colony-forming cells (ECFCs) are endothelial progenitor cells with robust proliferative potential. Their profound vessel-forming capacity makes them a promising tool for innovative experimental, diagnostic, and therapeutic strategies. Efficient and safe methods for their isolation and expansion are presently lacking. Based on the previously established efficacy of animal serum-free large-scale clinical-grade propagation of mesenchymal stromal cells, we hypothesized that endothelial lineage cells may also be propagated efficiently following a comparable strategy. Here we demonstrate that human ECFCs can be recovered directly from unmanipulated whole blood. A novel large-scale animal protein-free humanized expansion strategy preserves the progenitor hierarchy with sustained proliferation potential of more than 30 population doublings. By applying large-scale propagated ECFCs in various test systems, we observed vascular networks in vitro and perfused vessels in vivo. After large-scale expansion and cryopreservation phenotype, function, proliferation, and genomic stability were maintained. For the first time, proliferative, functional, and storable ECFCs propagated under humanized conditions can be explored in terms of their therapeutic applicability and risk profile.
Factors influencing lesion formation during radiofrequency catheter ablation. (2003)

In radiofrequency (RF) ablation, the heating of cardiac tissue is mainly resistive. RF current heats cardiac tissue and in turn the catheter electrode is being heated. Consequently, the catheter tip temperature is always lower--or ideally equal--than the superficial tissue temperature. The lesion size is influenced by many parameters such as delivered RF power, electrode length, electrode orientation, blood flow and tissue contact. This review describes the influence of these different parameters on lesion formation and provides recommendations for different catheter types on selectable parameters such as target temperatures, power limits and RF durations.
Lymphokines and cytokines as cancer treatment.Immunotherapy realized (1990)

Human proteins with identified effects on host responses to malignant cells have been established as effective therapeutic techniques in cancer. Lymphokines, products of activated cells of the immune system, have pleiotropic biochemical and cellular effects. These include stimulation of immune effector cell proliferation, augmentation of cytotoxicity of immune effector cells for tumor cell targets, enhancement in antigen-recognition potential by monocytes, and modulation of tumor-associated antigen expression on neoplastic cells. Interferons (IFN) and interleukin-2 (IL-2), purified to homogeneity, can induce regression of metastatic malignancy. Recombinant DNA technology has facilitated large-scale production of these and other lymphokines and cytokines. It has also made possible analyses of physical structures of the molecules themselves and has enabled creation of mutated molecules with specific, desired substitutions in their amino acid sequence. Monoclonal antibodies, directed at tumor-associated antigens, can augment antibody-dependent cell-mediated cytotoxicity and can selectively deliver cytotoxic techniques to malignant cells. Molecules that modify the host resistance to malignant disease also have potential to augment effectiveness of other cancer treatment techniques. Lymphokines, cytokines, and monoclonal antibodies, all products of biotechnology, have resulted in fulfillment of the promise of the immune system for inhibition of growth of human malignancy.
Understanding Fault Characteristics of Inverter-Based Distributed Energy Resources (2010)

This report discusses issues and provides solutions for dealing with fault current contributions from inverter-based distributed energy resources.
Online Learning in a Chemical Perceptron (2013)

Autonomous learning implemented purely by means of a synthetic chemical system has not been previously realized. Learning promotes reusability and minimizes the system design to simple input-output specification. In this article we introduce a chemical perceptron, the first full-featured implementation of a perceptron in an artificial (simulated) chemistry. A perceptron is the simplest system capable of learning, inspired by the functioning of a biological neuron. Our artificial chemistry is deterministic and discrete-time, and follows Michaelis-Menten kinetics. We present two models, the weight-loop perceptron and the weight-race perceptron, which represent two possible strategies for a chemical implementation of linear integration and threshold. Both chemical perceptrons can successfully identify all 14 linearly separable two-input logic functions and maintain high robustness against rate-constant perturbations. We suggest that DNA strand displacement could, in principle, provide an implementation substrate for our model, allowing the chemical perceptron to perform reusable, programmable, and adaptable wet biochemical computing.
Role of αvβ3-integrin in TNF-α-induced endothelial cell migration (2002)

Tumor necrosis factor-alpha (TNF-alpha), one of the major inflammatory cytokines, is known to influence endothelial cell migration. In this study, we demonstrate that exposure of calf pulmonary artery endothelial cells to TNF-alpha caused an increase in the formation of membrane protrusions and cell migration. Fluorescence microscopy revealed an increase in alpha(v)beta(3) focal contacts but a decrease in alpha(5)beta(1) focal contacts in TNF-alpha-treated cells. In addition, both cell-surface and total cellular expression of alpha(v)beta(3)-integrins increased significantly, whereas the expression of alpha(5)beta(1)-integrins was unaltered. Only focal contacts containing alpha(v)beta(3)- but not alpha(5)beta(1)-integrins were present in membrane protrusions of cells at the migration front. In contrast, robust focal contacts containing alpha(5)beta(1)-integrins were present in cells behind the migration front. A blocking antibody to alpha(v)beta(3), but not a blocking antibody to alpha(5)-integrins, significantly inhibited TNF-alpha-induced cell migration. These results indicate that in response to TNF-alpha, endothelial cells may increase the activation and ligation of alpha(v)beta(3) while decreasing the activation and ligation of alpha(5)beta(1)-integrins to facilitate cell migration, a process essential for vascular wound healing and angiogenesis.
CYCLOSPORIN A (1979)

The in vitro action of the immunosuppressive agent Cyclospirin A has been investigated using porcine cells. Lymphocyte proliferation induced in response to transplantation antigens and phytohaemagglutinin (PHA), was inhibited by this agent at doses that failed to inhibit mitogenisis in response to sheep anti-pig IgM, growth of kidney cell monolayers, and leukocyte migration.
Retinal prosthesis to aid the visually impaired (2003)

In this retinal prosthesis project, a rehabilitative device is being designed to replace the functionality of defective photoreceptors in patients suffering from retinal pigmentosa and age-related macular degeneration. An implantable intraocular unit receives power and signal via a telemetric inductive link with an extraocular unit. The extraocular unit consists of a video camera and video processing board, a telemetry protocol encoder chip, an RF amplifier and a primary coil, while the intraocular unit consists of a secondary coil, a rectifier and regulator, a retinal chip with a telemetry protocol decoder and stimulus signal generator, and an electrode array. This paper describes the design and testing of the overall system components.
Isolation of kidney complementary DNAs down-expressed in Wilms' tumor by a subtractive hybridization approach. (1993)

We applied a subtractive hybridization approach to isolate genes differentially expressed between mature kidney and Wilms' tumor. We constructed a complementary DNA library from a total mature kidney complementary DNA subtracted by an excess of mRNA from a Wilms' tumor, WAGR4, with a germline deletion of 11p13 and a somatic loss of alleles at 11p15. Six clones presenting a differential pattern of expression, positive with mRNA from the mature kidney and negative with mRNA from the Wilms' tumor WAGR4, were characterized. Among these clones were two as yet unknown expressed sequences (D11S877E and D15S109E) and four sequences from known genes: renal dipeptidase (DPEP1), alpha B-crystallin (CRYA2), uromodulin (UMOD), and plasma glutathione peroxidase (GPX2). The different patterns of expression of these genes in 11 Wilms' tumors, whether or not they are hereditary, reflect the well-documented pathogenetic heterogeneity for Wilms' tumors. We propose that these clones could be helpful for an improved histological characterization of Wilms' tumors.
Local wound care in diabetic foot complications (1999)

Techniques to prevent and treat lower extremity amputation in patients with diabetes vary from simple foot inspection to complicated vascular and reconstructive surgery. Early identification of risk factors, careful and regular evaluation, and aggressive treatment in a multidisciplinary team approach prevent amputation in most cases of diabetic foot ulcer. Suitable treatment of these ulcers consists of minimizing pressure, resolving infection, correcting ischemia, and maintaining a warm, moist, clean environment to enhance wound healing. Success in these efforts not only preserves quality of life for diabetic patients but also saves money for the healthcare system.
Prostate carcinoma (2003)

The development and progression of a prostate carcinoma from prediagnosis to death can be characterized as a series of clinical states. The states are milestones that can be used to assess prognosis, define therapeutic objectives, and assess outcomes. The antitumor effects of hormone therapies and cytotoxic agents in patients with prostate carcinoma are placed in context along with the bidrectional tumor-host interactions that contribute to the growth and resistance of osseous lesions. Identifying the factors that contribute to the growth of the disease at different points in the illness has lead to novel, systemic approaches. Proving the benefit of these approaches requires a series of unique trials with unique endpoints relevant to the clinical state of the patients and the specific therapy under evaluation.
Porphyrins with Four Monodisperse Oligocarbazole Arms:  Facile Synthesis and Photophysical Properties (2008)

A series of novel monodisperse, well-defined, star-shaped molecules T(OCAn)Ps (n = 2-6) with a central porphyrin core and four oligocarbazole arms are synthesized from the corresponding formyl-substituted oligocarbazoles via Adler reaction. The obtained star-shaped porphyrins are intrinsically two-dimensional nanosized molecules, and the diameter of compound T(OCA6)P is 7.4 nm, representing one of the largest known star-shaped conjugated systems. Their photophysical properties have been investigated by absorption and steady-state fluorescence spectroscopy, together with the corresponding monodisperse oligocarbazole aldehyde precursors. It is found that the light-harvesting capability of T(OCAn)Ps increases with the increasing length of the arms and reaches the maximum when n = 6. A selective excitation of the oligocarbazole arms leads to the typical emission from the porphyrin cores, indicating occurrence of photoinduced intramolecular energy transfer, and the energy transfer efficiency decreases from T(OCA2)P to T(OCA6)P owing to the Förster energy-transfer process. Accordingly, the longest effective distance for Förster energy transfer is estimated to be ca. 3 nm in our system. Such star-shaped porphyrins may find applications in photonic devices, with respect to their intense emission of red light. Notably, the monodisperse oligocarbazole aldehyde precursors give twisted intramolecular charge-transfer (TICT) excited states and luminescence in polar solvents with large Stokes shifts.
Integrating a perceptual information infrastructure with robotic avatars: a framework for tele-existence (2003)

Describes an infrastructural framework to support interactivity among entities that are not residing in the same physical environment. In such an environment, mobile robots supported by a perceptual information infrastructure gather information and communicate with people in a distant place instead of the users. This is one of the possible and promising applications of robots based on the current technologies of robotics, multimedia and computer networks. The paper describes the overall architecture and main system components. We discuss experimental studies where underlying concepts and algorithms are evaluated.