gebhart/patrepeval huggingface dataset:
Oxidative damage to plasma constituents by ozone (1992)

The reaction of ozone (O3) with human blood plasma was studied to help understand possible events that could occur in the respiratory tract. Uric acid (quantitatively the most important scavenger) and ascorbic acid were oxidized quickly, protein-SH groups were lost more slowly, and there was no loss of bilirubin or alpha-tocopherol. There was little formation of lipid hydroperoxides and no detectable formation of 4-hydroxynoneal, hexanal or nonanal, or changes in lipoprotein electrophoretic mobility. Uric acid in human upper airway secretions may play a significant role in removing inhaled O3. Oxidative damage to lipids must not be assumed to be the key mechanism of respiratory tract O3 toxicity.
Electrical Wiring of Glucose Oxidase by Reconstitution of FAD-Modified Monolayers Assembled onto Au-Electrodes (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTElectrical Wiring of Glucose Oxidase by Reconstitution of FAD-Modified Monolayers Assembled onto Au-ElectrodesItamar Willner, Vered Heleg-Shabtai, Ron Blonder, Eugenii Katz, Guoliang Tao, Andreas F. Bückmann, and Adam HellerView Author Information Institute of Chemistry The Hebrew University of Jerusalem Jerusalem 91904, Israel Gesellschaft für Biotechnologische Forschung Department of Enzymology Mascheroder Weg 1 D-38124 Braunschweig, Germany Department of Chemical Engineering University of Texas at Austin Austin, Texas 78712-1062 Cite this: J. Am. Chem. Soc. 1996, 118, 42, 10321–10322Publication Date (Web):October 23, 1996Publication History Received18 March 1996Published online23 October 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja9608611https://doi.org/10.1021/ja9608611rapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views2774Altmetric-Citations327LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Carbohydrates,Electrodes,Monolayers,Oxidation,Peptides and proteins Get e-Alerts
Impaired Chronotropic and Vasodilator Reserves Limit Exercise Capacity in Patients With Heart Failure and a Preserved Ejection Fraction (2006)

HFpEF patients have reduced chronotropic, vasodilator, and cardiac output reserve during exercise compared with matched subjects with hypertensive cardiac hypertrophy. These limitations cannot be ascribed to diastolic abnormalities per se and may provide novel therapeutic targets for interventions to improve exercise capacity in this disorder.
Quantitative microtiter cytotoxicity assay for Shigella toxin (1980)

The cytotoxic activity of Shigella dysenteriae 1 was assayed by exposing HeLa cells in microtiter cultures to dilutions of toxin. Exposure to toxin caused either failure of cells in suspension to attach or detachment of cells from established monolayers. Estimates of toxin potency were made by staining residual cells with crystal violet and visually inspecting the stained plates. Quantitation of the cytotoxic effect was made possible by eluting and spectrophotometrically measuring the stain. The dilution of toxin causing 50% cell detachment, the endpoint chosen for the assay, was estimated from plots of dye absorbance versus toxin dilution. The 50% cell detachment dilution of toxin varied as a function of cell concentration, incubation of toxin with cells in suspension or as established monolayers, and the cell line used for assay. The HeLa cell line was the most sensitive of the cell lines examined. The method was easily utilized to monitor toxin purification and to measure antitoxin neutralization of toxin activity.
Thymine DNA Glycosylase Is a Positive Regulator of Wnt Signaling in Colorectal Cancer (2014)

Wnt signaling plays an important role in colorectal cancer (CRC). Although the mechanisms of β-catenin degradation have been well studied, the mechanism by which β-catenin activates transcription is still not fully understood. While screening a panel of DNA demethylases, we found that thymine DNA glycosylase (TDG) up-regulated Wnt signaling. TDG interacts with the transcription factor TCF4 and coactivator CREB-binding protein/p300 in the Wnt pathway. Knocking down TDG by shRNAs inhibited the proliferation of CRC cells in vitro and in vivo. In CRC patients, TDG levels were significantly higher in tumor tissues than in the adjacent normal tissues. These results suggest that TDG warrants consideration as a potential biomarker for CRC and as a target for CRC treatment.
Brazilian Lignocellulosic Wastes for Bioenergy Production: Characterization and Comparison with Fossil Fuels (2013)

The aim of this paper was to analyze energy-related properties of forestry and agricultural wastes for energy production purposes, and to compare them with fossil fuels. The forestry wastes used were red cedar, Eucalyptus, and Pinus wood shavings. The agricultural wastes analyzed were rice husk, coffee wastes, sugar cane bagasse, maize harvesting wastes, and bamboo cellulose pulp. The forestry wastes presented more suitable properties for bioenergy production than the agricultural wastes. Desirable energetic properties were found for coffee wastes. The opposite was verified for rice husks. Among the biomass studied, coffee wastes presented the highest equivalent in fossil fuel volume and hence may lead to the highest decrease in CO2 emissions by fossil fuels used in Brazil for steam and heat production. The results suggests that CO2 benefits can be obtained if bioenergy is generated in the same locale where biomass is produced, avoiding CO2 cost of logistics and leading to greater end-use efficiency. The present work promotes the widespread use of different lignocellulosic wastes for bioenergy production and gives useful information for the planning and the control of power plants using biomass.
Enhanced internal dynamics of a membrane transport protein during substrate translocation (2000)

Conformational changes are essential for the activity of many proteins. If, or how fast, internal fluctuations are related to slow conformational changes that mediate protein function is not understood. In this study, we measure internal fluctuations of the transport protein lactose permease in the presence and absence of substrate by tryptophan fluorescence spectroscopy. We demonstrate that nanosecond fluctuations of alpha-helices are enhanced when the enzyme transports substrate. This correlates with previously published kinetic data from transport measurements showing that millisecond conformational transitions of the substrate-loaded carrier are faster than those in the absence of substrate. These findings corroborate the hypothesis of the hierarchical model of protein dynamics that predicts that slow conformational transitions are based on fast, thermally activated internal motions.
Synthesis and Physicochemical Characterization of End-Linked Poly(ethylene glycol)-co-peptide Hydrogels Formed by Michael-Type Addition (2003)

The synthesis of novel hybrid hydrogels by stepwise copolymerization of multiarm vinyl sulfone-terminated poly(ethylene glycol) macromers and alpha-omega cysteine oligopeptides via Michael-type additions is described. Cross-linking kinetics, studied by in situ rheometry, can be controlled by pH and the presence of charged amino acid residues in close proximity to the Cys, which modulates the pK(a) of the thiol group. These end-linked networks were characterized by their equilibrium swelling in water, by their viscoelastic properties in the swollen state, and by their soluble fraction. It was demonstrated that structure and properties are very sensitive to the preparation state including stoichiometry and precursor concentration and less sensitive to the pH during cross-linking. For each network the concentration of elastically active chains (nu) was calculated from experimentally determined sol fractions using Miller-Macosko theory and compared to values obtained from swelling and rheometry studies and by calculation from Flory's classical network models. Hydrogels were also prepared with varying macromer structures, and their properties were shown to respond to both macromer functionality and molecular weight.
Sildenafil Improves Exercise Hemodynamics in Fontan Patients (2014)

In Fontan patients, sildenafil improved cardiac index during exercise with a decrease in total pulmonary resistance index and an increase in stroke volume index. This implies that pulmonary vasculature represents a physiological limitation, which can be attenuated by sildenafil, the clinical significance of which warrants further study.
Planning, geometry, and complexity of robot motion (1986)

Robotics has come to attract the attention of mathematicians and theoretical computer scientists to a rapidly increasing degree. Initial investigations have shown that robotics is a rich source of deep theoretical problems, which range over computational geometry, control theory, and many aspects of physics, and whose solutions draw upon methods developed in subjects as diverse as automata theory, algebraic topology, and Fourier analysis. Also presented is some of this theoretical robotics research, emphasizing work relating to the geometric aspects of robot motion planning.
Wide-angle laser structured light system calibration with a planar object (2010)

In this paper, we proposed a calibration method for a structured light system composed of a wide-angle line laser and a camera using a planar object. Instead of directly calibrating the camera with a planar object, we separated the radial distortion estimation from the camera calibration step to improve accuracy. Firstly, we corrected the lens radial distortion and then computed the camera's intrinsic and extrinsic parameters. For line laser calibration, the line laser's position was extracted from the distorted image. The laser's sub-pixel position was then calculated using the proposed normalized weighted average method, and forward-mapped to the rectified image. The corresponding 3D point of the extracted laser was estimated by computing the intersecting point of the camera ray and the calibration plane. The experimental results show the superiority of the proposed method.
A zinc-sensing receptor triggers the release of intracellular Ca 2+ and regulates ion transport (2001)

Changes in extracellular zinc concentration participate in modulating fundamental cellular processes such as proliferation, secretion, and ion transport in a mechanism that is not well understood. Here, we show that a micromolar concentration of extracellular zinc triggers a massive release of calcium from thapsigargin-sensitive intracellular pools in the colonocytic cell line HT29. Calcium release was blocked by a phospholipase-C inhibitor, indicating that formation of inositol 1,4,5-triphosphate is required for zinc-dependent calcium release. Zinc influx was not observed, indicating that extracellular zinc triggered the release. The Ca(i)2+ release was zinc specific and could not be triggered by other heavy metals. Furthermore, zinc failed to activate the Ca(2+)-sensing receptor heterologously expressed in HEK293 cells. The zinc-induced Ca(i)2+ rise stimulated the activity of the Na(+)/H(+) exchanger in HT29 cells. Our results indicate that a previously uncharacterized extracellular, G protein-coupled, Zn(2+)-sensing receptor is functional in colonocytes. Because Ca(i)2+ rise is known to regulate key cellular and signal-transduction processes, the zinc-sensing receptor may provide the missing link between extracellular zinc concentration changes and the regulation of cellular processes.
Increased expression of the amyloid precursor β‐secretase in Alzheimer's disease (2002)

Beta-secretase cleavage represents the first step in the generation of Abeta polypeptides and initiates the amyloid cascade that leads to neurodegeneration in Alzheimer's disease. By comparative Western blot analysis, we show a 2.7-fold increase in protein expression of the beta-secretase enzyme BACE in the brain cortex of Alzheimer's disease patients as compared to age-matched controls. Similarly, we found the levels of the amyloid precursor protein C-terminal fragment produced by beta-secretase to be increased by nearly twofold in Alzheimer's disease cortex.
Treatment of non-inflamed obstructive meibomian gland dysfunction by an infrared warm compression device (2002)

The IWCD was effective and safe for the treatment of MGD. Improved tear stability associated with release of meibum is a possible mechanism of this treatment.
Newborn Metabolic Screening (1986)

The rationale for metabolic screening includes discovery of new diseases, development of treatment methods, determination of the true incidence of disease in order to provide genetic counseling, and relief to the taxpayer when retardation is preventable. The number of diseases for which we can now screen is large and growing and must be periodically reviewed. Physical, cultural, and religious factors affect gene pool mixing, making it impossible to predict a priori what the incidence of a given disorder will be within the population screened. This paper reviews the rationale for establishing neonatal metabolic screening programs and the significance of the information gained.
Detection of adenovirus‐specific T cells in children with adenovirus infection after allogeneic stem cell transplantation (2005)

Human adenovirus (ADV) infection is an important complication post allogeneic stem cell transplantation (SCT), especially in children, with significant morbidity and mortality despite new antiviral treatment strategies. Although the control of infection seems to require T cells, characterization of ADV-specific T cells post-SCT has not been reported to date. Therefore, we prospectively studied the occurrence of ADV-specific T cells in children with (n = 21) and without (n = 25) ADV-infection postallogeneic SCT and in healthy donors (n = 53). ADV-associated mortality occurred in seven of 21 children. After stimulation ex vivo with ADV-lysate, interferon-gamma-secreting T cells were analysed by flow cytometry. All the patients with ADV-associated mortality had no specific T cells, although reconstitution of absolute lymphocyte counts exceeded 0.3 x 10(9)/l within 30 d post-transplant. Patients who cleared ADV infection had significantly higher frequencies (mean +/- standard deviation, 0.56 +/- 0.5%) of ADV-specific T cells until day 200 post-SCT, than patients without ADV-infection (0.12 +/- 0.1%). These data suggest that ADV-specific T-cell reconstitution is protective against life-threatening ADV-infections postallogeneic SCT.
Über die Einwirkung von Phthalyl‐acylchloriden auf Benzol und Chlor‐aluminium (1913)

Ernst Pfaehler: mer die Elinwirkung von Phthalyl-
Synthesis and Biological Evaluation of Purine-Containing Butenolides (2001)

6-Chloropurine derivatives of gamma-(Z)-ethylidene-2,3-dimethoxybutenolide 3a, gamma-(Z)-ethylidene-2-methoxy-3-(4-nitro)benzyloxybutenolide 3b, gamma-(Z)-ethylidene-2-(4-nitro)benzyloxy-3-methoxybutenolide 3c, gamma-(Z)-ethylidene-2,3-di(4-nitro)benzyloxybutenolide 3d, and dimethylphosphono-gamma-(Z)-ethylidene-2,3-dimethoxybutenolide 11 as well as the adenine derivative of gamma-(Z)-ethylidene-2,3-dimethoxybutenolide 6 were synthesized. The key steps in the high-yield synthesis of 6 involved hydration/dehydration of the C(4)=C(5) in the precursor 3a. In the presence of NH4OH at elevated temperature, 3a underwent a reverse Michael-type addition with water to produce hydrate 5. At 37 degrees C, 6 was also hydrated in the presence of S-adenosyl-L-homocysteine hydrolase to afford 5. Butenolide 6 exhibited an inhibitory property toward the enzyme. Such type II (enzyme-mediated addition of water across C(4)=C(5)) mechanism is the first example of "enzyme-substrate intermediate" inactivation of S-adenosyl-L-homocysteine hydrolase. In contrast with type I mechanism-based inactivation, reduction of enzyme-bound NADP(+) to NADPH was not observed. Upon treatment with HCl, stereoselective dehydration of 5 occurred to give the target molecule 6. At ambident temperature, 3a was hydrated in the presence of NH4OH or pig liver esterase to produce 6-chloropurine derivative 4. An unambiguous proof of the structures of 3-5 was obtained by X-ray crystallographic analysis. For the synthesis of phosphonate derivative 11, the key step involved chlorination of phosphonate 9 by use of CF3SO2Cl and 1,8-diazabicyclo[5.4.0]undec-7-ene in CH2Cl2. 6-Chloropurine-containing butenolide 3d, 6-chloropurine derivative of 4-hydroxybutenolide 4, and adenine-containing 4-hydroxybutenolide 5 did not show anticancer and antiviral activities. 6-Chloropurine-containing ethylidene-2,3-dialkoxybutenolides 3a-c and phosphonate 11, however, exhibited inhibitory activity against murine leukemias (L1210 and P388), breast carcinoma (MCF7), and human T-lymphoblasts (Molt4/C8 and CEM/0) cell lines. They were also notably active toward thymidine kinase-deficient varicella-zoster virus (TK(-)VZV). Adenine-containing ethylidene-2,3-dimethoxybutenolide 6 exhibited marked selectivity in cytostatic activity against the murine leukemia (P388) cell line.
Adrenomedullin and the Blood-Brain Barrier (2001)

Adrenomedullin (ADM) is present both in the periphery and brain. In addition to its peripheral effects, this peptide can exert central effects such as decreasing food ingestion. We used multiple-time regression analysis to determine that labeled ADM can cross from blood to brain with an apparent influx constant (K(I)) of 5.83 +/- 1.44 x 10(-4) ml/g-min, much faster than that of albumin, the vascular control. HPLC showed that almost all of the injected 125I-ADM in the brain was intact, and capillary depletion showed that it could reach the parenchyma of the brain. However, more 125I-ADM was reversibly associated with the brain vasculature than we have seen with any other peptide tested by these methods. After intracerebroventricular injection, 125I-ADM exited the brain with the bulk reabsorption of cerebrospinal fluid at an efflux rate comparable to that of albumin. Although there was no blood-to-brain saturation, in situ brain perfusion of 125I-ADM in blood-free physiological buffer showed self-inhibition by excess unlabeled ADM. This, along with evidence of the lack of protein binding shown by capillary zone electrophoresis, indicated competition for the binding site of ADM at the BBB. The low lipophilicity of ADM determined by the octanol/buffer partition coefficient was also consistent with the prominent reversible association of ADM with the vasculature of the BBB. This suggests a function for ADM at the cerebral blood vessels, such as altering cerebral blood flow and perfusion, without disruption of the BBB.
Synergistic Supramolecular Encapsulation of Amphiphilic Hyperbranched Polymer to Dyes (2006)

A novel synergistic effect during supramolecular host−guest encapsulations of core−shell amphiphilic hyperbranched polymers to two kinds of dyes was reported. Two kinds of palmityl chloride-modified hyperbranched polymer, polyamidoamine (HPAMAM10K-C16) and poly(sulfone−amine) (HPSA11K-C16), were used as the hosts of supramolecular encapsulations, and water-soluble dyes such as methyl orange (MO) and methyl blue (MB) were selected as the corresponding guests. In cases of single-dye encapsulations, each host can extract one kind of guest from water phase into chloroform phase with a certain loading capacity (Cload). In the double-dye encapsulations, a pair of dyes, MO and MB, was employed as the guests. The Cload increased significantly for one or both of the pair dyes with the Cload of the single-dye encapsulation as the reference. This is defined as synergistic encapsulation in this paper. For the HPAMAM10K-C16, Cload of MO can be increased by about 100% with the cooperation of MB. For HPSA11K-C16, Cload of MB can be raised to a 40−100-fold level of single-dye encapsulation without loss of MO. Experiments with different encapsulating sequence showed that the synergistic encapsulation for HPAMAM10K-C16 is a parallel-type process because the sequence has no significant impact on the Cload of each dye, while the case of HPSA11K-C16 is a cascade-type process since the results with different encapsulating order were different. The synergistic encapsulation phenomenon was confirmed by the measurements of 1H NMR, DSC, and TGA on the resulting dye-encapsulated hyperbranched polymers. In addition, investigations on the guest−host supramolecular systems with different pH indicated that the pH value has certain influence on the synergistic encapsulation Cload, but it was not the dominating factor for the synergistic encapsulation. The synergistic effect was found simultaneously in the supramolecular encapsulation of two hosts, indicating this effect is not limited to a peculiar dendritic polymer. The phenomenon presented here will trigger further application of dendritic and other complex polymeric materials in the supramolecular host−guest chemistry.
Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression (2007)

The study of T regulatory cells (T reg cells) has been limited by the lack of specific surface markers and an inability to define mechanisms of suppression. We show that the expression of CD39/ENTPD1 in concert with CD73/ecto-5'-nucleotidase distinguishes CD4(+)/CD25(+)/Foxp3(+) T reg cells from other T cells. These ectoenzymes generate pericellular adenosine from extracellular nucleotides. The coordinated expression of CD39/CD73 on T reg cells and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops, indicating roles in the inhibitory function of T reg cells. Consequently, T reg cells from Cd39-null mice show impaired suppressive properties in vitro and fail to block allograft rejection in vivo. We conclude that CD39 and CD73 are surface markers of T reg cells that impart a specific biochemical signature characterized by adenosine generation that has functional relevance for cellular immunoregulation.
Indonesia in Crisis (1998)

For more about the East-West Center, see <a href="http://www.eastwestcenter.org/">http://www.eastwestcenter.org/</a>
<title>Silicon Liquid Crystal Light Valves For Optical-Data Processing</title> (1982)

The structure and operation of two novel liquid crystal light valves (LCLV) are described: the photo activated, silicon-photoconductor-based LCLV and the electronically-addressed CCD-LCLV. The current status of both devices are detailed. Applications of these devices for signal and image processing are given.