gebhart/patrepeval huggingface dataset:
Characterization and crystallization of core streptavidin. (1987)

We have characterized a streptavidin product that had been reduced to a minimal size that still retained full biotin-binding activity. This core streptavidin is proteolyzed at both ends at points that correspond closely with the termini of hen egg white avidin. Core streptavidin is more soluble than is the parent molecule. We have grown three different types of crystals of core streptavidin. The symmetry properties of these crystals prove that the molecule is a tetramer organized in tetrahedral (D2) point symmetry. The crystallographic response to the interaction of biotin with core streptavidin indicates that some conformational change accompanies ligand binding. We are attempting to determine the three-dimensional structure of streptavidin and its complex with selenobiotin from these crystals of core streptavidin.
Effects of the Use of Non-Calcium Phosphate Binders in the Control and Outcome of Vascular Calcifications: A Review of Clinical Trials on CKD Patients (2011)

Vascular calcifications produce a high impact on morbidity and mortality rates in patients affected by chronic kidney disease and mineral bone disorder (CKD-MBD). Effects are manifested from the more advanced stages of CKD (stages 3-4), particularly in patients undergoing dialysis (CKD5D). In recent years, a large number of therapeutic options have been successfully used in the treatment of secondary hyperparathyroidism (SHPT), despite eliciting less marked effects on nonbone calcifications associated with CKD-MBD. In addition to the use of Vitamin D and analogues, more recently treatment with calcimimetic drugs has also been undertaken. The present paper aims to analyze comparative and efficacy studies undertaken to assess particularly the impact on morbidity and mortality rates of non-calcium phosphate binders. Moreover, the mechanism of action underlying the depositing of calcium and phosphate along blood vessel walls, irrespective of the specific contribution provided in reducing the typical phosphate levels observed in CKD largely at more advanced stages of the disease, will be investigated. The aim of this paper therefore is to evaluate which phosphate binders are characterised by the above action and the mechanisms through which these are manifested.
Cardioprotection by ε-Protein Kinase C Activation From Ischemia (2004)

The epsilonPKC activator psi(epsilon)RACK induced cardioprotection both in vivo and ex vivo, reduced the incidence of lethal arrhythmia during ischemia-reperfusion, and did not cause desensitization or downregulation of epsilonPKC after sustained delivery. Thus, psi(epsilon)RACK may be useful for patients with ischemic heart disease. In addition, the psi(epsilon)RACK peptide should be a useful pharmacological agent for animal studies in which systemic and sustained modulation of epsilonPKC in vivo is needed.
Interaction of psoralen-derivatized oligodeoxyribonucleoside methylphosphonates with single-stranded DNA (1988)

Oligodeoxyribonucleoside methylphosphonates derivatized at the 5' end with 4'-(amino-alkyl)-4,5',8-trimethylpsoralen were prepared. The interaction of these psoralen-derivatized methylphosphonate oligomers with synthetic single-stranded DNAs 35 nucleotides in length was studied. Irradiation of a solution containing the 35-mer and its complementary methylphosphonate oligomer at 365 nm gave a cross-linked duplex produced by cycloaddition between the psoralen pyrone ring of the derivatized methylphosphonate oligomer and a thymine base of the DNA. Photoadduct formation could be reversed by irradiation at 254 nm. The rate and extent of cross-linking were dependent upon the length of the aminoalkyl linker between the trimethylpsoralen group and the 5' end of the methylphosphonate oligomer. Methylphosphonate oligomers derivatized with 4'-[[N-(2-aminoethyl)amino]methyl]- 4,5',8-trimethylpsoralen gave between 70% and 85% cross-linked product when irradiated for 20 min at 4 degrees C. Further irradiation did not increase cross-linking, and preirradiation of the psoralen-derivatized methylphosphonate oligomer at 365 nm reduced or prevented cross-linking. These results suggest that the methylphosphonate oligomers undergo both cross-linking and deactivation reactions when irradiated at 365 nm. The extent of cross-linking increased up to 10 microM oligomer concentration and dramatically decreased at temperatures above the estimated Tm of the methylphosphonate oligomer-DNA duplex. The cross-linking reaction was dependent upon the fidelity of base-pairing interactions between the methylphosphonate oligomers and the single-stranded DNA. Noncomplementary oligomers did not cross-link, and the extent of cross-linking of oligomers containing varying numbers of noncomplementary bases was greatly diminished or eliminated.(ABSTRACT TRUNCATED AT 250 WORDS)
Copper Is Required to Maintain Cu/Zn-Superoxide Dismutase Activity During HL-60 Cell Differentiation (1993)

The objective of these studies was to characterize the relationship between copper levels and Cu/Zn superoxide dismutase (Cu/Zn-SOD) during cellular differentiation. It was hypothesized that the decrease in Cu/Zn-SOD activity that accompanied differentiation would be reversed by supplementing the culture medium with copper. HL-60 cells, a human promyelocytic cell line, were induced to differentiate with retinoic acid and were concurrently supplemented with copper or a copper chelator, tetraethylenepentamine. The results showed that retinoic acid-treated cells contained more copper after differentiation. When the medium was supplemented with copper during retinoic acid treatment, the differentiating cells accumulated more copper than the nondifferentiating cells. Differentiation was accompanied by a significant reduction in Cu/Zn-SOD activity and a slight reduction in Cu/Zn-SOD protein. Activity returned to control values when an extracellular source of copper was provided. Incubation of retinoic acid-treated cells with the chelator showed that they lost proportionally less copper than the noninduced controls. Levels of Cu/Zn-SOD protein were not affected by the copper or chelator treatments. It was concluded that the requirement of differentiating HL-60 cells for copper is not related to providing copper for Cu/Zn-SOD activity. If a supplemental source is not supplied in the medium, then the cells may acquire copper from an intracellular source, namely Cu/Zn-SOD.
Communication and Concurrency (1989)

Foreword. 1. Modelling Communication. 2. Basic Definitions. 3. Equational laws and Their Application. 4. Strong Bisimulation and Strong Equivalence. 5. Bisimulation and Observation Equivalence. 6. Further Examples. 7. The Theory of Observation Congruence. 8. Defining a Programming Language. 9. Operators and Calculi. 10. Specifications and Logic. 11. Determinancy and Confluence. 12. Sources and Related Work. Bibliography. Index.
DEMOCRACIA O DICTADURA, DISYUNTIVA DE CENTROAMERICA (1981)

EL DOCTOR MARIO SOLORZANO MARTINEZ, CATEDRATICO DE LA UNIVERSIDAD DE COSTA RICA, DURANTE EL TRANSCURSO DE LA CONFERENCIA QUE DIO TERMINO AL CICLO DENOMINADO SITUACION ACTUAL DE CENTROAMERICA: ANALISIS Y PERSPECTIVAS, MANIFESTO QUE EL DESPERTAR DE LOS PUEBLOS CENTROAMERICANOS ES EL RESULTADO DE LA ACUMULACION DE VERDADERAS AFRENTAS PUBLICAS QUE, SUMADAS UNA A UNA, HAN DADO LUGAR A MULTIPLES CONTRADICIONES (ECONOMICAS, SOCIALES POLITICAS Y CULTURALES) SE HAYAN CONCENTRADO EN UNA SOLA QUE PUEDE REUNIRSE ASI: DEMOCRACIA O DICTADURA. AGREGO QUE EL ENFOQUE DADO POR LA ADMINISTRACION DE REAGAN A LA CUESTION CENTROAMERICANA, ES RECHAZADO POR LA MAYORIA DE LOS PUEBLOS DEL MUNDO Y RECIBIDO CON DESCONFIANZA, INCLUSO POR IMPORTANTES ALIADOS DE LOS ESTADOS UNIDOS.
Implications of silicon monolithic RFICs for medical instrumentation and telemetry (2002)

Today, much of the focus regarding the use of radio frequency integrated circuits (RFICs) lies in the realm of audio, video and general data transmission for the consumer sector. Although medical applications are seldom mentioned in either the trade press or professional journals, they likewise represent some very challenging, beneficial, and potentially high-volume applications. This paper describes a variety of possible medical uses for RFIC-based sensor/transceivers, including the treatment and management of patients with cardiac abnormalities, seizure disorders, and diabetes, as well as touching on the areas of pediatric thermometry and sleep apnea. Discussion of the role of RF identification (RFID) tags in the hospital environment is also included, as well as a brief look at some of the practical, legal and economic considerations involved in the use of RF-based sensors.
Association of defective HLA-I expression with antigen processing machinery and their association with clinicopathological characteristics in Kazak patients with esophageal cancer. (2011)

Our results suggest that APM component defects are a mechanism underlying HLA-I antigen down-regulation in ESCC lesions, and indicate that the loss expression of HLA-I and APM components will become an important marker of ESCC and analysis of HLA-I and APM component expression can provide useful prognostic information for patients with ESCC from the Kazak ethnic group.
Pilot study of Flt3 ligand comparing intraperitoneal with subcutaneous routes on hematologic and immunologic responses in patients with peritoneal carcinomatosis and mesotheliomas. (2003)

Flt3-L, administered either i.p. or s.c., is well tolerated and produced similar increases in monocytes, DCs, and platelets. DCs from peripheral blood and peritoneal fluids showed cell surface phenotypic and cytokine maturational responses to activation stimuli. These findings suggested that Flt3-L, in combination with suitable activating agents, could be developed further in patients with epithelial ovarian cancer.
Glucagon-Like Peptide-1 and Exendin-4 Stimulate β-Cell Neogenesis in Streptozotocin-Treated Newborn Rats Resulting in Persistently Improved Glucose Homeostasis at Adult Age (2001)

In neonatal Wistar rats injected with streptozotocin (STZ) at birth (n0-STZ model), a recognized model of beta-cell regeneration, we investigated the capacity of early treatment with glucagon-like peptide 1 (GLP-1) or exendin-4 to promote beta-cell regeneration and thereby improve islet function in the long term, when animals become adults. To this end, n0-STZ rats were submitted to GLP-1 or exendin-4 from postnatal day 2 to day 6 only, and their beta-cell mass and pancreatic functions were tested on day 7 and at 2 months. On day 7, both treatments increased body weight, decreased basal plasma glucose, decreased insulinemia, and increased pancreatic insulin content in n0-STZ rats. At the same age, the beta-cell mass, measured by immunocytochemistry and morphometry methods, was strongly increased in n0-STZ/GLP-1 and n0-STZ/Ex rats compared with n0-STZ rats, representing 51 and 71%, respectively, of the beta-cell mass in Wistar rats, whereas n0-STZ beta-cell mass represented only 21% of the Wistar control value. Despite such early improved beta-cell mass, which is maintained at adult age, the basal and glucose-stimulated insulin secretion (in vivo after intravenous glucose load or in vitro using perfused pancreas) were not improved in the 2-month-old n0-STZ rats previously treated with GLP-1 or exendin-4 compared with untreated n0-STZ rats. However, both treated groups significantly exhibited a decreased basal plasma glucose level and an increased plasma glucose clearance rate compared with the 2-month-old untreated n0-STZ group at adult age. These findings in the n0-STZ model indicate for the first time that GLP-1 or exendin-4 applied during the neonatal diabetic period exert both short- and long-term beneficial effects on beta-cell mass recovery and glucose homeostasis. However, the increase in beta-cell mass, which is still present in the adult n0-STZ rats previously treated, contrasts with the poor beta-cell responsiveness to glucose. Further studies are needed to understand the dissociation between beta-cell regeneration and the lack of improvement in beta-cell function.
A Survey on Optical Interconnects for Data Centers (2012)

Data centers are experiencing an exponential increase in the amount of network traffic that they have to sustain due to cloud computing and several emerging web applications. To face this network load, large data centers are required with thousands of servers interconnected with high bandwidth switches. Current data center networks, based on electronic packet switches, consume excessive power to handle the increased communication bandwidth of emerging applications. Optical interconnects have gained attention recently as a promising solution offering high throughput, low latency and reduced energy consumption compared to current networks based on commodity switches. This paper presents a thorough survey on optical interconnects for next generation data center networks. Furthermore, the paper provides a qualitative categorization and comparison of the proposed schemes based on their main features such as connectivity and scalability. Finally, the paper discusses the cost and the power consumption of these schemes that are of primary importance in the future data center networks.
Enhanced Coalbed Methane Recovery (1990)

Abstract At present, coalbed methane is being recovered by means of reservoir pressure depletion. While this method is simple and effective, it is not efficient. Reduction in reservoir pressure deprives the fluids of the energy necessary to flow to the wellbore. Furthermore, there is a practical and economic limit on the extent to which reservoir pressure can be reduced. It is estimated that reservoir pressure depletion strategy of coalbed methane production will permit the recovery of 50% or less of the gas-in-place. This paper discusses an alternate method of coalbed methane recovery which consists of nitrogen flooding a coal seam. Lab research has shown that essentially all methane sorbed on coal can be stripped by nitrogen without necessarily reducing the total system pressure. That is, methane desorption from coal is achieved by reducing the partial pressure of methane rather than merely the total pressure. Lab and modeling results are presented to demonstrate how nitrogen injection in coal can accelerate methane production rates and enhance reserves. Scoping process economics of enhanced coalbed methane production also appear to be attractive.
Sodium cromoglycate in childhood asthma (2001)

The review of placebo controlled trials of sodium cromoglycate (SCG) in childhood asthma by Tasche et al 1 concludes that “there is insufficient evidence that SCG has a beneficial effect as maintenance treatment in children with asthma”.

We do not believe this conclusion is justified. The principal criteria for assessing the efficacy of inhaled SCG are the mean differences in the effect on the symptoms of cough and wheeze between SCG and placebo across all trials included in the review. The 95% confidence intervals for these differences are 0.12 to 0.27 for cough and 0.11 to 0.26 for wheeze. As the differences are in favour of SCG and the confidence intervals do not include zero, the only conclusion that can be drawn is that there is strong statistical evidence for a beneficial effect of SCG in children with asthma—the reverse conclusion to that presented.

The review identified 24 randomised controlled trials and is claimed to include “all published randomised, placebo controlled trials”. Two published trials are not included (those by Berman et al 2 and Mikawa et al 3) and the trial by Silverman et al ,4 which is included, does not meet the author's criteria. Both excluded trials, which involved 472 children, reported statistically significant differences in favour of SCG compared with placebo. The study by Silverman et al compared the combination of inhaled SCG and isoprenaline with isoprenaline alone and did not include a placebo comparison.

The authors have calculated a tolerance distribution for study effects and we believe it is this distribution which has been fundamentally misinterpreted. They provide a tolerance interval for the results of future studies based on the between study variation seen. The fact that this interval contains zero does not mean that there is no evidence of a difference between treatments, as …
Using AAV vectors expressing the β2-adrenoceptor or associated Gα proteins to modulate skeletal muscle mass and muscle fibre size (2016)

Anabolic β2-adrenoceptor (β2-AR) agonists have been proposed as therapeutics for treating muscle wasting but concerns regarding possible off-target effects have hampered their use. We investigated whether β2-AR-mediated signalling could be modulated in skeletal muscle via gene delivery to the target tissue, thereby avoiding the risks of β2-AR agonists. In mice, intramuscular administration of a recombinant adeno-associated virus-based vector (rAAV vector) expressing the β2-AR increased muscle mass by >20% within 4 weeks. This hypertrophic response was comparable to that of 4 weeks' treatment with the β2-AR agonist formoterol, and was not ablated by mTOR inhibition. Increasing expression of inhibitory (Gαi2) and stimulatory (GαsL) G-protein subunits produced minor atrophic and hypertrophic changes in muscle mass, respectively. Furthermore, Gαi2 over-expression prevented AAV:β2-AR mediated hypertrophy. Introduction of the non-muscle Gαs isoform, GαsXL elicited hypertrophy comparable to that achieved by AAV:β2-AR. Moreover, GαsXL gene delivery was found to be capable of inducing hypertrophy in the muscles of mice lacking functional β1- and β2-ARs. These findings demonstrate that gene therapy-based interventions targeting the β2-AR pathway can promote skeletal muscle hypertrophy independent of ligand administration, and highlight novel methods for potentially modulating muscle mass in settings of disease.
Asialoglycoprotein receptor (ASGPR): a peculiar target of liver-specific autoimmunity (2012)

Asialoglycoprotein receptor (ASGPR) autoantibodies have been considered specific markers of autoimmune hepatitis (AIH). The exact mechanisms responsible for the development of these autoantibodies and leading to autoimmunity to this peculiar liver receptor remain elusive. Furthermore, loss of T cell tolerance to ASGPR has been demonstrated in patients with AIH, but it is poorly understood whether such liver-specific T cell responses bear a pathogenic potential and/or participate in the precipitation of AIH. Newly developed enzyme-linked immunosorbent assays have led to the investigation of the sensitivity and specificity of anti-ASGPR antibodies for AIH. The present review provides an overview of the diagnostic and clinical relevance of anti-ASGPR antibodies. A thorough investigation of the autoreactivity against ASGPR may assist efforts to understand liver autoimmunity in susceptible individuals.
ROLE OF G PROTEINS IN SIGNAL TRANSDUCTION (1992)

Molecular Pathophysiology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, U.S.A.
New Genetic Loci That Control Susceptibility and Symptoms of Experimental Allergic Encephalomyelitis in Inbred Mice (1998)

Experimental allergic encephalomyelitis (EAE), the principal animal model of multiple sclerosis, is a genetically determined phenotype. In this study, analyses of the cumulative disease frequencies in parental, F1 hybrid, and F2 mice, derived from the EAE-susceptible SJL/J strain and the EAE-resistant B10.S/DvTe strain, confirmed that susceptibility to EAE is not inherited as a simple Mendelian trait. Whole genome scanning, using 150 informative microsatellite markers and a panel of 291 affected and 390 unaffected F2 progeny, revealed significant linkage of EAE susceptibility to marker loci on chromosomes 7 (eae4) and 17, distal to H2 (eae5). Quantitative trait loci for EAE severity, duration, and onset were identified on chromosomes 11 (eae6, and eae7), 2 (eae8), 9 (eae9), and 3 (eae10). While each locus reported in this study is important in susceptibility or disease course, interactions between marker loci were not statistically significant in models of genetic control. One locus, eae7, colocalizes to the same region of chromosome II as Orch3 and Idd4, susceptibility loci in autoimmune orchitis and insulin-dependent diabetes mellitus, respectively. Importantly, eae5 and eae7 are syntenic with human chromosomes 6p21 and 17q22, respectively, two regions of potential significance recently identified in human multiple sclerosis genome scans.
Evaluation of size effect on mechanical properties of single crystal silicon by nanoscale bending test using AFM (2000)

This paper describes a nanometer-scale bending test for a single crystal silicon (Si) fixed beam using an atomic force microscope (AFM). This research focuses on revealing the size effect on the mechanical property of Si beams ranging from a nano- to millimeter scale. Nanometer-scale Si beams, with widths from 200 to 800 nm and a thickness of 255 nm, were fabricated on an Si diaphragm by means of field-enhanced anodization using AFM and anisotropic wet etching. The efficient condition of the field-enhanced anodization could be obtained by changing the bias voltage and the scanning speed of the cantilever. Bending tests for micro- and millimeter-scale Si beams fabricated by a photolithography technique were also carried out using an ultraprecision hardness tester and scratch tester, respectively. Comparisons of Young's modulus and bending strength, of Si among the nano-, micro-, and millimeter scales showed that the specimen size did not have an influence on the Young's modulus in the [110] direction, whereas it produced a large effect on the bending strength. Observations of the fractured surface and calculations of the clack length from Griffith's theory made it clear that the maximum peak-to-valley distance of specimen surface caused the size effect on the bending strength.
A portable clock multiplier generator using digital CMOS standard cells (1996)

High frequency clock rate is a key issue in today's VLSI. To improve performance on-chip, clock multipliers are used. But it is a difficult task to design such circuits while maintaining low cost. This paper presents a circuit fabricated to test a new method of clock frequency multiplication. This new approach uses a digital CMOS process in order to implement a fully integrated digital delay locked loop. This multiplier does not require external components. Moreover, as it is primarily intended for ASIC design, it is generated by a parameterized generator written in C which relies on a portable digital standard cell library for automatic place and route. The design based on the delay locked loop allows the clock waveform to reach its operating point faster than conventional methods. Special techniques enable high multiplication factors (between 4 and 20) without compromising the timing accuracy. With a clock multiplier of 20, in 1 /spl mu/m CMOS process and a 5 V supply voltage, a 170 MHz clock signal has been obtained from a 8.5 MHz external clock with a measured jitter lower than 300 ps.
Metalorganic chemical vapor deposition of III-V semiconductors (1985)

The metalorganic chemical vapor deposition (MOCVD) of epitaxial III-V semiconductor alloys on III-V substrates is reviewed in detail. The emphasis is placed on both practical and theoretical knowledge of the equipment and deposition process. The chemistry of the source alkyls and the dynamics of the transport process are discussed. The growth of the GaAs and AlxGa1−xAs systems are treated as prototypical examples (and the most studied) of the III-V materials. Latter sections review InP, Ga1−xInxAs, and related alloys. Finally, the antimonide and the other systems are reviewed. Electronic and optical devices fabricated from MOCVD-grown materials are used as examples of the capabilities of the growth technique.