gebhart/patrepeval huggingface dataset:
Technique for fast and reproducible fingerprinting of bacteria by pyrolysis mass spectrometry (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTechnique for fast and reproducible fingerprinting of bacteria by pyrolysis mass spectrometryH L C. Meuzelaar and P G. KistemakerCite this: Anal. Chem. 1973, 45, 3, 587–590Publication Date (Print):March 1, 1973Publication History Published online1 May 2002Published inissue 1 March 1973https://pubs.acs.org/doi/10.1021/ac60325a051https://doi.org/10.1021/ac60325a051research-articleACS PublicationsRequest reuse permissionsArticle Views255Altmetric-Citations126LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
General formulas for 2-D leaky-wave antennas (2005)

General formulas for a two-dimensional (2-D) leaky-wave antenna (LWA) are obtained, which are applicable to any general class of 2-D leaky-wave antenna that consists of a partially reflecting surface that is mounted on top of a grounded substrate, and excited by a simple source. Closed-form expressions are obtained for the radiated fields, the field peak values in the E- and H-planes, the beamwidths in the E- and H-planes, and the pattern bandwidth. The formulas are obtained from a simple transverse equivalent network model of the structure. An accurate formula for the substrate thickness necessary to give a beam at any desired angle is also obtained.
Protein protocols on CD-ROM (1998)

Protein Protocols on CD-ROM provides instant access to over 2500 time-tested state-of-the-art protein methods and supporting protocols that are unfailingly reproducible and easily accessible through CD-ROM technology. Unprecedented in scope and depth of
Collagen COL4A3 knockout: a mouse model for autosomal Alport syndrome. (1996)

A mouse model for the autosomal form of Alport syndrome was produced. These mice develop a progressive glomerulonephritis with microhematuria and proteinuria, consistent with the human disease. End-stage renal disease develops at approximately 14 weeks of age. TEM analysis of the glomerular basement membranes (GBM) during development of renal pathology revealed focal multilaminated thickening and thinning beginning in the external capillary loops at 4 weeks and spreading throughout the GBM by 8 weeks. By 14 weeks, half of the glomeruli were fibrotic with collapsed capillaries. Immunofluorescence analysis of the GBM showed the absence of type IV collagen alpha-3, alpha-4, and alpha-5 chains and a persistence of alpha-1 and alpha-2 chains (these chains normally localize to the mesangial matrix). Northern blot analysis using probes specific for the collagen chains illustrate the absence of COL4A3 in the knockout, whereas mRNAs for the remaining chains are unchanged. An accumulation of fibronectin, heparan sulfate proteoglycan, laminin-1, and entactin was observed in the GBM of the affected animals. The temporal and spatial pattern of accumulation was consistent with that for thickening of the GBM as observed by TEM. Thus, expression of these basement membrane-associated proteins may be involved in the progression of Alport renal disease pathogenesis. The levels of mRNAs encoding the basement membrane-associated proteins at 7 weeks were unchanged.
A numerically efficient damping model for acoustic resonances in microfluidic cavities (2015)

Bulk acoustic wave devices are typically operated in a resonant state to achieve enhanced acoustic amplitudes and high acoustofluidic forces for the manipulation of microparticles. Among other loss mechanisms related to the structural parts of acoustofluidic devices, damping in the fluidic cavity is a crucial factor that limits the attainable acoustic amplitudes. In the analytical part of this study, we quantify all relevant loss mechanisms related to the fluid inside acoustofluidic micro-devices. Subsequently, a numerical analysis of the time-harmonic visco-acoustic and thermo-visco-acoustic equations is carried out to verify the analytical results for 2D and 3D examples. The damping results are fitted into the framework of classical linear acoustics to set up a numerically efficient device model. For this purpose, all damping effects are combined into an acoustofluidic loss factor. Since some components of the acoustofluidic loss factor depend on the acoustic mode shape in the fluid cavity, we propose a two-step simulation procedure. In the first step, the loss factors are deduced from the simulated mode shape. Subsequently, a second simulation is invoked, taking all losses into account. Owing to its computational efficiency, the presented numerical device model is of great relevance for the simulation of acoustofluidic particle manipulation by means of acoustic radiation forces or acoustic streaming. For the first time, accurate 3D simulations of realistic micro-devices for the quantitative prediction of pressure amplitudes and the related acoustofluidic forces become feasible.
Clinical effectiveness of a dentifrice containing potassium chloride as a desensitizing agent. (1992)

Sensodyne F, a dentifrice currently marketed in the United Kingdom, containing potassium chloride (KCl) and sodium monofluorophosphate (MFP) was compared to a placebo dentifrice for effectiveness in alleviating dentinal hypersensitivity. This randomized, double-blind, parallel clinical study covered 12 weeks of product use by 41 subjects. Hypersensitivity levels of the affected teeth were assessed by tactile stimulation, cold air stimulation and overall subjective patient response. The results from these three methods of assessment demonstrated that the KCl/MFP dentifrice was significantly more effective than the placebo dentifrice in reducing dentinal hypersensitivity. The therapeutic response to the KCl/MFP dentifrice as measured by air sensitivity and overall subjective evaluation was statistically significant when compared to the placebo dentifrice within 4 weeks of use. Significant improvement was observed for all parameters at the conclusion of the 12-week clinical study period. Plaque reduction was significantly reduced at week 8 and continued to improve by week 12. The results indicate that KCl with sodium MFP significantly reduced dentinal hypersensitivity and improved overall patient comfort.
Capacitorless Dynamic Random Access Memory Cell with Highly Scalable Surrounding Gate Structure (2007)

We propose the surrounding gate metal oxide semiconductor field effect transistor (MOSFET) with vertical channel [surrounding gate and vertical channel (SGVC) cell] as a one transistor (1T) dynamic random access memory (DRAM) cell. To confirm the memory operation of the SGVC cell, we fabricated a highly scalable SGVC cell. Because of its vertical channel structure and common source architecture, it can readily be made into a 4F2 cell array. The sensing margin was around 6 µA which is sufficiently large for read operation. Read retention time was about 4 ms meaning that the read operation is non-destructive. Also, we have revealed that not only an increase in the volume of the floating body region but also an adequate amount of charge is required for an increase in the sensing margin.
A microcontroller‐based implantable neuromuscular stimulation system with wireless power and data transmission for animal experiments (2003)

Abstract The aim of this study is to develop an implantable microcontroller‐based stimulation system with external wireless controller for data and power transmission. The system coupling efficiency can reach 35% in a reasonable range of distance via a self‐oscillating class E transmitter with power transmission efficiency up to 90%. The implanted micro‐stimulator is built on a double‐layer printed circuit board, which is 3 cm in diameter, by using SMD components mostly. Its overall power consumption is around 30 mW. Our results indicate the regulated voltage can be maintained at a tractable range when the coupling distance is within 30 mm. Within this distance, lateral displacement of the implant is allowed as long as it is inside the area of the transmitting coil. Meanwhile, a wide range of stimulation patterns can be generated for nerve stimulation and blocking for animal experiments. All the functional blocks of a prototype implantable neuromuscular system have been successfully designed, implanted, and tested which is valuable for future ASIC design for a miniature implantable microstimulator.
PTGF-β , a type β transforming growth factor (TGF-β) superfamily member, is a p53 target gene that inhibits tumor cell growth via TGF-β signaling pathway (2000)

Identification and characterization of p53 target genes would lead to a better understanding of p53 functions and p53-mediated signaling pathways. Two putative p53 binding sites were identified in the promoter of a gene encoding PTGF-beta, a type beta transforming growth factor (TGF-beta) superfamily member. Gel shift assay showed that p53 bound to both sites. Luciferase-coupled transactivation assay revealed that the gene promoter was activated in a p53 dose- as well as p53 binding site-dependent manner by wild-type p53 but not by several p53 mutants. The p53 binding and transactivation of the PTGF-beta promoter was enhanced by etoposide, a p53 activator, and was largely blocked by a dominant negative p53 mutant. Furthermore, expression of endogenous PTGF-beta was remarkably induced by etoposide in p53-positive, but not in p53-negative, cell lines. Finally, the conditioned medium collected from PTGF-beta-overexpressing cells, but not from the control cells, suppressed tumor cell growth. Growth suppression was not, however, seen in cells that lack functional TGF-beta receptors or Smad4, suggesting that PTGF-beta acts through the TGF-beta signaling pathway. Thus, PTGF-beta, a secretory protein, is a p53 target that could mediate p53-induced growth suppression in autocrinal as well as paracrinal fashions. The finding made a vertical connection between p53 and TGF-beta signaling pathways in controlling cell growth and implied a potential important role of p53 in inflammation regulation via PTGF-beta.
Ph.D. Education (1995)

The following sentence was omitted from the acknowledgment section of our report "Independent human MAP kinase signal transduction pathways defined by MEK and MKK isoforms" (3 Feb., p. 682)(1) because of an error. "A. Lin and M. Karin are acknowledged for informing us abut the presence of an upstream in-frame initiation codon in the sequence of human MKK4/JNKK/SEK1 before publication."
The metabolic syndrome (2004)

Summary The metabolic syndrome has been described as a ‘clustering’ of several risk factors for cardiovascular disease, namely obesity (particularly abdominal obesity), dyslipidaemia, insulin resistance and hypertension. Globally, the incidence of this syndrome is rising at an alarming rate. Treatments involve pharmacological therapies and diet and lifestyle changes. In order to reduce the incidence of this disease, and to minimise costs to health services, increasing emphasis must be placed on prevention, and on the education of health professionals and the public regarding the importance of diet and exercise in preventing this disease. Research is urgently required to elucidate the molecular mechanisms underlying this syndrome, to evaluate prognostic biomarkers identifying this disease and to isolate and evaluate potential therapeutic agents. The OB‐AGE and LIPGENE projects are European Union funded research projects which aim to enhance our understanding of this syndrome (at a whole body, cellular and molecular level) and to evaluate the effectiveness of dietary fatty acids in preventing/ameliorating this disease.
Composite functional metasurfaces for multispectral achromatic optics (2017)

Nanostructured metasurfaces offer unique capabilities for subwavelength control of optical waves. Based on this potential, a large number of metasurfaces have been proposed recently as alternatives to standard optical elements. In most cases, however, these elements suffer from large chromatic aberrations, thus limiting their usefulness for multiwavelength or broadband applications. Here, in order to alleviate the chromatic aberrations of individual diffractive elements, we introduce dense vertical stacking of independent metasurfaces, where each layer is made from a different material, and is optimally designed for a different spectral band. Using this approach, we demonstrate a triply red, green and blue achromatic metalens in the visible range. We further demonstrate functional beam shaping by a self-aligned integrated element for stimulated emission depletion microscopy and a lens that provides anomalous dispersive focusing. These demonstrations lead the way to the realization of ultra-thin superachromatic optical elements showing multiple functionalities-all in a single nanostructured ultra-thin element.
Global Comparison of the Membrane Subproteomes between a Multidrug-Resistant Acinetobacter baumannii Strain and a Reference Strain (2006)

Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins) were identified. In this analysis, we described the expression by this bacterium of RND-type efflux systems and some potential virulence factors. We then compared the membrane subproteome of a clinical multidrug-resistant (MDR) isolate with that of a reference strain. We found that the MDR strain expressed lower levels of the penicillin-binding-protein 1b, produced a CarO protein having different primary and quaternary structures to that of the reference strain, and expressed OmpW isoforms. We also showed that the clinical strain has a high ability to form biofilms consistent with the accumulation of some outer membrane proteins (OMPs) such as NlpE or CsuD that have already been described as involved in bacterial adhesion. These features may partly explain the MDR emergence of the clinical isolate.
The science of hyaluronic acid dermal fillers (2008)

Knowledge of the chemical and physical blueprint of HA dermal fillers may help physicians in choosing the appropriate HA dermal filler for facial enhancements. This, together with appropriate injector training and injection experience, should lead to results that ultimately will benefit patients.
Third Activity of Bordetella Adenylate Cyclase (AC) Toxin-Hemolysin (2006)

The Bordetella adenylate cyclase toxin-hemolysin (CyaA) targets phagocytes expressing the alpha(M)beta2 integrin (CD11b/CD18), permeabilizes their membranes by forming small cation-selective pores, and delivers into cells a calmodulin-activated adenylate cyclase (AC) enzyme that dissipates cytosolic ATP into cAMP. We describe here a third activity of CyaA that yields elevation of cytosolic calcium concentration ([Ca2+]i) in target cells. The CyaA-mediated [Ca2+]i increase in CD11b+ J774A.1 monocytes was inhibited by extracellular La3+ ions but not by nifedipine, SK&F 96365, flunarizine, 2-aminoethyl diphenylborinate, or thapsigargin, suggesting that influx of Ca2+ into cells was not because of receptor signaling or opening of conventional calcium channels by cAMP. Compared with intact CyaA, a CyaA-AC- toxoid unable to generate cAMP promoted a faster, albeit transient, elevation of [Ca2+]i. This was not because of cell permeabilization by the CyaA hemolysin pores, because a mutant exhibiting a strongly enhanced pore-forming activity (CyaA-E509K/E516K), but unable to deliver the AC domain into cells, was also unable to elicit a [Ca2+]i increase. Further mutations interfering with AC translocation into cells, such as proline substitutions of glutamate residues 509 or 570 or deletion of the AC domain as such, reduced or ablated the [Ca2+]i-elevating capacity of CyaA. Moreover, structural alterations within the AC domain, because of insertion of various oligopeptides, differently modulated the kinetics and extent of Ca2+ influx elicited by the respective AC- toxoids. Hence, the translocating AC polypeptide itself appears to participate in formation of a novel type of membrane path for calcium ions, contributing to action of CyaA in an unexpected manner.
Epitope Discovery and Their Use in Peptide Based Vaccines (2010)

With recent advances in the design and delivery of peptide-based therapeutics there has been a growing interest on the use of peptides in vaccine design. Moreover, functional dissection and proteomic analysis of the immunogenic epitopes of proteins from pathogenic micro-organisms, cancers and self-tissues targeted by autoimmune responses, have broadened the range of target epitopes and given clues to enhancing peptide immunogenicity. Consistent with these observations; peptides can be synthesised with defined chemical modifications to mimic natural epitopes and/or deliberately introduce protease resistant peptide bonds to regulate their processing independent of tissue specific proteolysis and to stabilize these compounds in vivo. We discuss the potential of peptide-based vaccines for the treatment of chronic viral diseases and cancer and review recent developments in the field of epitope discovery and peptide-based vaccines.
Dermal fluid translocation is an important determinant of the diurnal variation in human skin thickness (2001)

The diurnal profiles of skin thickness and skin elasticity in the upper half of the body are the reverse of those in the lower half of the body. These findings suggest that shifts of dermal fluid from the face to the leg by gravity during the day cause the diurnal variation in skin thickness.
Cdc7 Kinase Is a Predictor of Survival and a Novel Therapeutic Target in Epithelial Ovarian Carcinoma (2009)

Our findings show that Cdc7 kinase predicts survival and is a potent anticancer target in epithelial ovarian carcinoma, highlighting its potential as a predictor of susceptibility to small molecule kinase inhibitors currently in development.
Alpha-v Integrins as Therapeutic Targets in Oncology (2007)

Integrins are heterodimeric cell adhesion receptors that mediate intercellular communication through cell-extracellular matrix interactions and cell-cell interactions. Integrins have been demonstrated to play a direct role in cancer progression, specifically in tumor cell survival, tumor angiogenesis, and metastasis. Therefore, agents targeted against integrin function have potential as effective anticancer therapies. Numerous anti-integrin agents, including monoclonal antibodies and small-molecule inhibitors, are in clinical development for the treatment of solid and hematologic tumors. This review focuses on the role of alpha(v) integrins in cancer progression, the current status of integrin-targeted agents in development, and strategies for the clinical development of anti-integrin therapies.
Design, Synthesis, and Preclinical Characterization of the Selective Androgen Receptor Modulator (SARM) RAD140 (2010)

This report describes the discovery of RAD140, a potent, orally bioavailable, nonsteroidal selective androgen receptor modulator (SARM). The characterization of RAD140 in several preclinical models of anabolic androgen action is also described.
Geographic load balancing for scalable distributed Web systems (2002)

Users of highly popular Web sites may experience long delays when accessing information. Upgrading content site infrastructure from a single node to a locally distributed Web cluster composed by multiple server nodes provides limited relief, because the cluster wide-area connectivity may become the bottleneck. A better solution is to distribute Web clusters over the Internet by placing content nodes in strategic locations. A geographically distributed architecture where the Domain Name System (DNS) servers evaluate network proximity and users are served from the closest cluster reduces network impact on response time. On the other hand, serving closest requests only may cause unbalanced servers and may increase system impact on response time. To achieve a scalable Web system, we propose to integrate DNS proximity scheduling with an HTTP request redirection mechanism that any Web server can activate. We demonstrate through simulation experiments that this further dispatching mechanism augments the percentage of requests with guaranteed response time, thereby enhancing the Quality of Service of geographically distributed Web sites. However, HTTP request redirection should be used selectively because the additional round-trip increases network impact on latency time experienced by users. As a further contribution, this paper proposes and compares various mechanisms to limit reassignments with no negative consequences on load balancing.
The Morbillivirus Receptor SLAM (CD150) (2002)

Morbilliviruses are highly contagious pathogens that cause some of the most devastating viral diseases of humans and animals, including measles virus (MV), canine distemper virus (CDV), and rinderpest virus (RPV). They replicate mainly in lymphoid organs throughout the body and cause severe immunosuppression accompanied with lymphopenia. We have recently shown that human, canine, and bovine signaling lymphocyte activation molecules (SLAMs; also known as CD150) act as cellular receptors for MV, CDV, and RPV, respectively. In these three morbilliviruses, all strains examined were shown to use SLAMs of their respective host species, and laboratory strains passaged on SLAM-negative cells were found to use, besides SLAM, alternative receptors, such as human CD46 for the Edmonston strain of MV. The use of SLAM as a receptor may be a property common to most, if not all, of the members of morbilliviruses. Human SLAM is a membrane glycoprotein selectively expressed on the cells of the immune system (immature thymocytes, activated lymphocytes, activated monocytes, and mature dendritic cells) and seems to mediate lymphocyte activation and to control interferon-gamma production. The destruction and/or impairment of infected SLAM-positive cells may be a mechanism for the immunosuppression induced by morbilliviruses, but other mechanisms may be also involved.
Nucleotide sequence ofClostridium difficiletoxin B gene (1990)

Clostridium difficile, the causative agent of antibiotic-associated pseudomembranous colitis, produces two toxins, A and B (1).