gebhart/patrepeval huggingface dataset:
Relation between yield and reliability of integrated circuits: experimental results and application to continuous early failure rate reduction programs (1996)

The relation between yield and early failures as encountered in the field was investigated with several high volume IC's manufactured in several processes. A clear correlation between yield and reliability was found that obeys a simple model. The model also provides the ability to predict the FIT or PPM level of a given IC based on its yield. Using these results we illustrate how a wafer fab may improve the reliability level of its products in a controlled way.
Cloning and sequence analysis of cDNA encoding rabbit vitamin D-binding protein (Gc globulin). (1994)

The cDNA sequence of rabbit vitamin D-binding protein (DBP) was determined by sequencing clones isolated by immuno-screening and PCR amplification from a rabbit liver cDNA library. The 1641-nucleotide sequence included the 5' untranslated region and extended to the poly(A) tail. It encoded a protein of 460 amino acid residues in addition to a signal sequence of 16 residues, and had 84%, 79% and 78% sequence identities with human, rat and mouse DBP cDNA, respectively. In comparison with DBP amino acid sequences of other species, there are at least four heterogeneous regions. In contrast, a putative actin binding domain was conserved in the evolution process.
Loss of Apc+ in intestinal adenomas from Min mice. (1994)

Allelic loss at the Apc locus in spontaneously occurring intestinal adenomas from mice heterozygous for the ApcMin nonsense mutation was analyzed using a site-specific quantitative polymerase chain reaction assay. All 97 of the intestinal adenomas analyzed showed extensive loss of the wild-type Apc (Apc+) allele. Quantitative polymerase chain reaction analysis of loci linked to Apc indicated loss of the chromosome carrying Apc+. Only one copy of the homologue carrying ApcMin remained in the intestinal adenomas. Possible reasons for the difference in the mechanism of Apc+ loss between human and Min mouse intestinal adenomas are discussed.
Inhibition of HDAC6 Attenuates Tumor Growth of Non-Small Cell Lung Cancer (2019)

Histone deacetylase 6 (HDAC6) regulates cytoplasmic signaling networks through deacetylation of various cytoplasmic substrates and serves as a key member of the ubiquitin proteasome system (UPS). This study is focused on HDAC6 regulation of the Notch1 receptor that plays a crucial role in tumor growth in NSCLC. A series of cell culture experiments were employed using A549, Lewis lung carcinoma 2 (LL2), and H1299 NSCLC cell lines to investigate HDAC6-mediated regulation of the Notch1 receptor through the UPS. HDAC6 was inhibited with small molecule inhibitors tubacin and ACY1215 in vitro and in vivo. Inhibition of HDAC6 led to reduced levels of Notch1 receptor in a dose-dependent manner in all three NSCLC cell lines tested. HDAC6 inhibition with ACY1215 led to G2 arrest, increased apoptosis, and increased levels of cleaved PARP1 in A549, LL2, and H1299 cell lines. In vivo inhibition of HDAC6 with ACY1215 significantly reduced LL2 tumor growth rate. Our data show that HDAC6 in NSCLC cells supports Notch1 signaling and promotes cell survival and proliferation. Our results support clinical investigation of HDAC6 inhibitors as a potential therapeutic option for treatment of NSCLC patients.
Plant male germ line transformation (1997)

Summary A new method to produce transgenic plants ‐ ma le ge rm li ne tr ansformation (MAGELITR) is reported. Unicellular tobacco microspores were isolated from excised anthers, and DNA carrying two marker genes was transferred biolistically. The bombarded microspores were matured in vitro for 6 days, and the mature pollen was used for in vivo pollination. Seeds were recovered and putative transformants were selected on the basis of their antibiotic resistance. Five kanamycin‐resistant plants were chosen for further analysis, four contained the first construct, one the second. Parallel experiments with bicellular immature pollen did not produce any transgenic plants. A detailed DNA blot and expression analysis confirmed the transgenic nature of the five plants, and a genetic analysis showed that the transgenes are transmitted to subsequent generations. MAGELITR is a fast, regeneration‐independent method, not prone to chimerism and somaclonal variation which should be genotype‐independent and may be applicable in a wide range of species once in vitro maturation of pollen is established.
IDENTIFICATION OF A NOVEL CELL TYPE IN PERIPHERAL LYMPHOID ORGANS OF MICE (1974)

Several properties of lymphoid dendritic cells in situ have been determined, and contrasted to information previously established for lymphocytes and mononuclear phagocytes. Dendritic cells are not found in newborn mice, and their concentration in both spleen and mesenteric lymph node does not reach adult levels until 3–4 wk of age. Dendritic cells largely disappear from adherent populations following administration of steroids (2.5 mg hydrocortisone acetate s.c.) and ionizing radiation (Do of 100 rads for Co60). Splenic dendritic cells can originate from precursors located in both bone marrow and spleen itself, probably the red pulp. The mature splenic population does not actively divide (pulse labeling index with [3H]thymidine of 1.5–2.5%), but does turnover at substantial rate, 10+% of the total pool per day. The influx of new cells appears to be derived from a proliferating precursor compartment, but the mechanism for efflux or turnover is not known. Dendritic cells in spleen and node undergo little or moderate increase in numbers during development of a primary immune response. These in vivo characteristics, taken together, further distinguish dendritic cells as a novel cell type, distinct from mononuclear phagocytes and lymphocytes.
Small-Format Drug Conjugates: A Viable Alternative to ADCs for Solid Tumours? (2018)

Antibody-Drug Conjugates (ADCs) have been through multiple cycles of technological innovation since the concept was first practically demonstrated ~40 years ago. Current technology is focusing on large, whole immunoglobulin formats (of which there are approaching 100 in clinical development), many with site-specifically conjugated payloads numbering 2 or 4. Despite the success of trastuzumab-emtansine in breast cancer, ADCs have generally failed to have an impact in solid tumours, leading many to explore alternative, smaller formats which have better penetrating properties as well as more rapid pharmacokinetics (PK). This review describes research and development progress over the last ~10 years obtained from the primary literature or conferences covering over a dozen different smaller format-drug conjugates from 80 kDa to around 1 kDa in total size. In general, these agents are potent in vitro, particularly more recent ones incorporating ultra-potent payloads such as auristatins or maytansinoids, but this potency profile changes when testing in vivo due to the more rapid clearance. Strategies to manipulate the PK properties, whilst retaining the more effective tumour penetrating properties could at last make small-format drug conjugates viable alternative therapeutics to the more established ADCs.
Diffuse continental deformation: length scales, rates and metamorphic evolution (1987)

Abstract In contrast with the oceanic portions of the plates, continents may deform hundreds of kilometres away from any plate boundary. Calculations that treat the continents as continuous media suggest that the across-strike length scale of deformation associated with a convergent boundary is proportional to the along-strike length of the boundary, the constant of proportionality depending on the rheology of the lithosphere. The dependence on convergent boundary length implies that, for fixed velocity of convergence, strain rates decrease with increasing length of the orogen. The dependence on rheology is less straightforward, but it appears from laboratory determination of flow laws that the scale length of compressional deformation decreases as the fraction of the lithospheric strength supported by friction on faults increases; that fraction depends on the thermal profile of the continental lithosphere and will change during the evolution of an orogenic belt. The thermal development of a diffusely deforming compressional belt is nearly independent of its strain history unless the strain rates are less than about 10-15s-1. If erosion terminates the heating phase of such an orogenic belt, the dominant control on the metamorphic history is the heat supply to the continental lithosphere. In contrast, if extension of the thickened crust ends metamorphism, peak metamorphic conditions depend only on the rheological properties of the lithosphere. Metamorphism in crust that is subject to major extension following compressional orogenesis should be nearly independent of the initial thermal conditions of the crust, and may be distinguished from metamorphism terminated by erosion by a final stage of isobaric cooling from temperatures close to the maximum experienced.
Tetracyclines May Be Therapeutically Beneficial in Rheumatoid Arthritis (1995)

Several clinical trials have been conducted in the past 3 years using minocycline as an anti-inflammatory agent for rheumatoid arthritis (RA), Depending on one's viewpoint, these trials have or have not shown a mild beneficial effect on conventional RA parameters of inflammation. In the context of currently available anti-inflammatory therapy, the magnitude of the reported effects would not seem to justify additional large scale trials. There is, however, compelling basic science data as well as preliminary clinical data, suggesting that certain tetracyclines may have a valuable role to play in treatment of RA. There is even reason to believe that such an agent could be the Holy Grail of rheumatology, namely the drug that prevents connective tissue degradation in destructive arthritides. This concept is based on the properties of tetracyclines as inhibitors of matrix metalloproteinases. In this article, the properties of the tetracyclines in this regard are reviewed, and concepts are suggested for the design of a trial addressing the unique properties of these drugs.
On-line image verification in radiation therapy: an early USA experience. (1993)

The intense effort in the past 5 to 10 years to develop on-line image verification (OLIV) systems is made partly in anticipation of the increased verification demands of complex 3-dimensional (3D) conformal radiation therapy, and partly to improve on the current practice of weekly treatment verification. These systems allow convenient acquisition of daily portal images or many images during one treatment session. Systems based on fluoroscopic and scanning approaches can now be purchased from various vendors and are being evaluated at several radiation therapy centers. The performances of these systems vary, but all appear to be adequate for clinical use. At the Mallinckrodt Institute of Radiology, we have been conducting clinical on-line imaging for the past 3 years using a fiber-optic fluoroscopic system. Daily operation of the system requires coordinated participation of the physics and radiation therapy technology staff. The major consideration is in the management and evaluation of the large amount of verification images about each individual patient. At present, it is not clear whether the technology would be cost-effective in the clinical setting. However, it is clear that OLIV provides a powerful tool in enhancing our knowledge of treatment variation. The information will be invaluable in the development of improved treatment techniques. It is also likely that these systems will play a important part in the verification of 3D conformal radiation therapy.
Multimodality therapy with a replication-conditional herpes simplex virus 1 mutant that expresses yeast cytosine deaminase for intratumoral conversion of 5-fluorocytosine to 5-fluorouracil. (2001)

Infection of tumor cells by herpes simplex virus 1 (HSV-1) results in cell destruction and production of progeny virion in a process referred to as viral oncolysis. In this study, an HSV-1 mutant (HSV1yCD) was engineered such that the viral ribonucleotide reductase gene is disrupted by sequences encoding yeast cytosine deaminase, which efficiently metabolizes the prodrug 5-fluorocytosine (5-FC) to 5-fluorouracil (5-FU). HSV1yCD-infected cells convert 5-FC to 5-FU, which enhances cytotoxicity without significantly reducing viral replication and oncolysis. Oncolysis by a replicating HSV-1 mutant combined with therapeutic transgene delivery represents a new paradigm; HSV1yCD-infected cells are destroyed by viral replication, and uninfected cells are subjected to bystander killing from both progeny virion and extracellular diffusion of 5-FU. In contrast, HSV1yCD-mediated bioactivation of another prodrug, ganciclovir, impairs viral replication. HSV1yCD administered into the portal venous system replicates preferentially in liver metastases rather than normal liver. The anti-neoplastic activity of HSV1yCD combined with systemic 5-FC administration is greater than that achieved with HSV-1 replication alone. Combination oncolysis and prodrug bioactivation leads to significant prolongation of survival in mice with diffuse liver metastases.
An algorithm for low memory wavelet image compression (2003)

As wavelet-based image coding is set to became more widely used (e.g. with the completion of the JPEG2000 standard), memory efficiency for wavelet-based coding is becoming an increasingly important issue. In this paper we present a complete system to perform low memory wavelet image coding. Our approach is "line-based" in that the images are read line by line and only the minimum required number of lines is kept in memory. The line-based transform is combined with a low memory entropy coder that does not require any global image information. Our system achieves a large (two orders of magnitude) reduction in memory requirements compared to other available coders, with limited performance loss (e.g., less than 0.5 dB).
System Optimization for Improved Accuracy in Transcutaneous Signal and Power Transmission (1984)

A stable transcutaneous transmission of power and signal via two coupled coils with minimized dependence on the relative spacing of external and implanted coil is possible by employing "critical coupling" between implanted and external circuits. Optimizing coil geometries and preventing the RF output amplifier from saturating is necessary to approximately maintain critical coupling despite placement tolerances within a reasonable range. Based on these considerations a transcutaneous signal transmission system for an auditory prosthesis has been designed. This transmission system can also be used for several other stimulation purposes needing accurate control of stimulation parameters.
Clinical trials in HIV-associated cognitive impairment: Cognitive and functional outcomes (2001)

Cognitive and functional outcomes are of primary interest in the design of efficacy trials in HIV-associated cognitive impairment. In a longitudinal cohort study, weak associations were found between measures of cognitive performance and commonly used measures of daily functioning (mostly self-report measures) in HIV-infected individuals. Modifications of current functional scales or new functional instruments are needed to assess the clinical relevance of cognitive changes in clinical trials of HIV-associated cognitive impairment.
Cyclooxygenase-2 and Epidermal Growth Factor Receptor: Pharmacologic Targets for Chemoprevention (2005)

Understanding the mechanisms underlying carcinogenesis provides insights that are necessary for the development of therapeutic strategies to prevent cancer. Chemoprevention, the use of drugs or natural substances to inhibit carcinogenesis, is a rapidly evolving aspect of cancer research. Evidence is presented that cyclooxygenase-2 (COX-2) and epidermal growth factor receptor (EGFR) are potential pharmacologic targets to prevent cancer. In this paper, we review key data implicating a causal relationship between COX-2, EGFR, and carcinogenesis and possible mechanisms of action. We discuss evidence of crosstalk between COX-2 and EGFR in order to strengthen the rationale for combination chemoprevention, and review plans for a clinical trial that will evaluate the concept of combination chemoprevention targeting COX-2 and EGFR.
Experimental respiratory syncytial virus infection of four species of primates (1977)

Four species of nonhuman primates were inoculated intranasally with 10(3.1) to 10(3.7) plaque forming units (pfu) of respiratory syncytial (RS) virus. Adults squirrel monkeys and newborn rhesus monkeys became infected and shed small quantities (peak titer 10(2.0) pfu/ml of nasopharyngeal swab specimen) of virus, but illness did not develop. Infant cebus monkeys aged 2 months became infected, shed 10(2.3) to 10(3.8) pfu/ml of nasopharyngeal swab specimen, but did not become ill. Chimpanzees aged 15 to 18 months shed a large quantity of virus, up to 10(6.0) pfu/ml of nasopharyngeal swab specimen and developed an upper respiratory illness. Chimpanzees are proposed as a possible animal model for future study of the immunopathology of RS virus disease and for in vivo evaluation of attenuated live virus vaccine candidates.
Extracellular matrix stimulation of guinea pig megakaryocyte proplatelet formation in vitro is mediated through the vitronectin receptor. (1992)

We have used an in vitro culture system to study the role of extracellular matrix (ECM) in the fragmentation of guinea pig bone marrow megakaryocytes (MK) and the formation of proplatelet fragments from these cells. Proplatelet formation is stimulated by culturing the cells on a hydrated type I collagen gel in the presence of serum. MK express integrin proteins alpha 5, alpha 6, beta 1, and the alpha v beta 3 complex as demonstrated by immunofluorescence. A monoclonal antibody, LM 609, to the alpha v beta 3 vitronectin receptor blocked proplatelet formation, whereas the monoclonal antibodies to the beta 1, alpha 5, and alpha 6 integrin proteins did not inhibit proplatelet formation. The tetrapeptide Arg-Gly-Asp-Ser (RGDS) inhibits proplatelet formation; however, there was no inhibition by the fibronectin receptor-specific peptide GRGDdSP. The fibrinogen gamma chain peptide HHLGGAKQAGDV, which binds to the platelet membrane glycoprotein complex IIb-IIIa but not to the vitronectin receptor (VnR), did not inhibit proplatelet formation, nor did two different laminin peptides. In the absence of serum, 5.7% of MK spontaneously formed processes or fragmented. The addition of 50 micrograms/ml of vitronectin to serum-free cultures increased proplatelet formation to 21.5% of the MK, equal to cultures with 10% serum. Stimulation of proplatelet formation by vitronectin in serum-free cultures was inhibited by LM 609. Antibody staining with anti-bovine vitronectin antibody showed that MK contain intracellular vitronectin. These data show that guinea pig MK express alpha 5, alpha 6, beta 1, and alpha v beta 3 integrins. However, only the MK vitronectin receptor and its interaction with vitronectin plays an essential role in proplatelet formation in vitro.
Static random access memories based on resonant interband tunneling diodes in the InAs/GaSb/AlSb material system (1995)

We have fabricated SRAM's based on resonant interband tunneling diodes in the InAs/AlSb/GaSb material system. The bistability and the switching principles are demonstrated. Numerical simulations of the memory characteristics of the SRAM cell are performed and used for comparing with experiments. Several key issues involving the applications of the device are also discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Heavy-Chain Grafted Antibody that Recognizes the Tumor-Associated TAG72 Antigen (1994)

Murine anti-TAG72 antibodies react with more than 85% of human colorectal, gastric and ovarian carcinomas. However, the therapeutic utility of murine antibodies in human is severely restricted by their immunogenicity. The construction of humanized antibodies can potentially circumvent this problem. The latter antibodies can be generated by grafting CDRs from a murine antibody onto human immunoglobulin FRs followed by combining the resultant product with human immunoglobulin constant regions. In this study, we constructed a heavy-chain humanized anti-TAG72 antibody that we designated hmM4. This was achieved by the transplantation of CDRs from the murine VH of the ccM4 antibody into FRs of the human myeloma protein NEWM. The humanized antibody hmM4 retained its binding reactivity for the TAG72 antigen as measured by ELISA and Western blotting analysis respectively. However, it showed considerably less immunoreactivity for the TAG72 antigen than the original chimeric antibody ccM4. These results indicate that the murine anti-TAG72 specificity can be grafted to human immunoglobulin, and that the choice of the human immunoglobulin framework for the grafted antibody may be critical in maintenance of the immunoreactivity.
A Bright and Specific Fluorescent Sensor for Mercury in Water, Cells, and Tissue (2007)

A bright idea: By restricting the rotation between receptor and reporter units of the fluorescent chemosensor Mercury Green 1 (MG1), a remarkably high quantum efficiency is achieved in its Hg2+-ion-bound form in water (Φ=0.72). MG1 is specific to environmentally and biologically relevant mercury levels (ppm to ppb range), and can assay levels of Hg2+ ion in living cells and in edible fish.
Ras pathway activation in gliomas: a strategic target for intranasal administration of perillyl alcohol (2008)

The preliminary results indicate that intranasal administration of the signal transduction inhibitor POH is a safe, noninvasive, and low-cost method. There were no toxicity events and the regression of tumor size in some patients is suggestive of antitumor activity.
Glutarate semialdehyde dehydrogenase of Pseudomonas. Purification, properties, and relation to L-lysine catabolism. (1977)

The lysine-induced glutarate semialdehyde dehydrogenase of Pseudomonas was purified to electrophoretic homogeneity from a mutant strain lacking delta-aminovalerate transaminase. The properties of the enzyme, including molecular weight, amino acid composition, electrophoretic behavior, and kinetic features, distinguish it from similar dehydrogenases induced in the same cell strain by hydroxyproline or by glucarate. Enzyme induction patterns and the growth behavior of a mutant deficient in glutarate semialdehyde dehydrogenase clearly relate this enzyme to the so-called delta-aminovalerate pathway of L-lysine catabolism. Induction studies also indicate that delta-aminovalerate is a better inducer of the dehydrogenase than L-lysine. Cells of a mutant strain lacking delta-aminovalerate transaminase contained higher levels of the dehydrogenase, presumably as a result of the accumulation of delta-aminovalerate, making this mutant a useful preparative source of the enzyme. The marked reduction of lysine-inducible glutarate semialdehyde dehydrogenase in a mutant strain permitted assessment of the basal levels of hydroxyproline/glucarate-inducible ketoglutarate semialdehyde dehydrogenases not possible in wild type cells.