gebhart/patrepeval huggingface dataset:
Follicular lymphomas can be induced to present alloantigen efficiently: a conceptual model to improve their tumor immunogenicity. (1995)

In the tumor-bearing host, T cells invariably fail to induce a clinically significant antitumor immune response. Although model systems support the existence of tumor peptide antigens, the molecular interactions critical for antigen presentation by the tumor cell remain unresolved. Here, we demonstrate that human follicular lymphoma cells are highly inefficient at presenting alloantigen despite their strong expression of major histocompatibility complex and low-to-intermediate expression of some adhesion and B7 costimulatory molecules. Activation of follicular lymphoma cells via CD40 induces or up-regulates both adhesion and B7 costimulatory molecules essential to repair this defect. More importantly, once primed, alloreactive T cells efficiently recognize unstimulated follicular lymphoma cells. Thus, correction of defective tumor immunity requires not only expression of major histocompatibility complex but also sufficient expression of multiple adhesion and costimulatory molecules.
Treatment of active psoriatic arthritis with the PPAR ligand pioglitazone: an open-label pilot study (2004)

Treatment with a PPARgamma agonist appears to be a promising therapeutic principle in PsA, but the use of PPARgamma ligands might be limited by side-effects such as increase in weight and fluid retention.
Tumor-induced apoptosis of T cells: amplification by a mitochondrial cascade. (2000)

We have recently reported that apoptosis of T cells induced by squamous cell carcinoma of the head and neck (SCCHN) is partly Fas dependent. This tumor-induced T-cell death is mediated by the activities of caspase-8 and caspase-3 and is partially inhibited by antibodies to either Fas or Fas ligand. We report here that in contrast to apoptosis induced by agonistic anti-Fas antibody (Ab), the tumor-induced apoptotic cascade in Jurkat cells is significantly amplified by a mitochondrial loop. The involvement of mitochondria in tumor-induced apoptosis of T cells was demonstrated by changes in mitochondrial permeability transition as assessed by 3,3'-dihexiloxadicarbocyanine staining, by cleavage of cytosolic BID and its translocation to the mitochondria, by release of cytochrome c to the cytosol, and by the presence of active subunits of caspase-9 in Jurkat T cells cocultured with tumor cells. To further elucidate the significance of mitochondria in tumor-induced T-cell death, we investigated the effects of various inhibitors of the mitochondrial pathway. Specific antioxidants, as well as two inhibitors of mitochondria permeability transition, bongkrekic acid and cyclosporin A, significantly blocked the DNA degradation induced in Jurkat T cells by SCCHN cells. However, these inhibitors had no effect on cells triggered by anti-Fas Ab. Furthermore, a cell-permeable inhibitor of caspase-9, Ac-LEHD.CHO, which did not inhibit T-cell apoptosis induced by anti-Fas Ab, markedly inhibited apoptosis induced by etoposide or by coculture of Jurkat with SCCHN cells. These findings demonstrate that apoptotic cascades induced in Jurkat T lymphocytes by anti-Fas Ab or tumor cells are differentially susceptible to a panel of inhibitors of mitochondrial apoptotic events. It appears that besides the Fas-mediated pathway, additional mitochondria-dependent cascades are involved in apoptosis of tumor-associated lymphocytes. Inhibition of mitochondria-dependent cascades of caspase activation should be considered to enhance the success of immunotherapy or vaccination protocols in cancer.
Gangliosides expressed in human breast cancer. (1996)

Breast tumors that were histopathologically diagnosed as invasive ductal carcinoma were examined in relation to their abnormal expression of gangliosides. Total ganglioside levels that were expressed as lipid-bound sialic acids were significantly higher in breast tumor tissues than in normal mammary tissues. Two kinds of unusual gangliosides were found to be expressed in many cases of breast tumors. One was a group of O-acetylated gangliosides, such as O-acetyl-GD3 and O-acetyl-GT3. They are known as fetal gangliosides, which appear in fetal brains. The other was an N-glycolylneuraminic acid-containing ganglioside, N-glycolyl-GM3, which had not been previously found in normal human tissues. The finding that unusual gangliosides are expressed in breast tumors may provide the basis for their immunological diagnosis and vaccine therapy.
The Brain Opera Technology: New Instruments and Gestural Sensors for Musical Interaction and Performance (1999)

Abstract This paper describes the array of new musical instruments and interactive installations developed for the Brain Opera, a large, touring multimedia production, where the audience first explores a set of musical modes at a variety of novel, interactive stations before experiencing them in an actual performance. Most of the Brain Opera's installations were intended for the general public, employing different gestural measurements and mappings that allow an untrained audience to intuitively interact with music and graphics at various levels of complexity. Another set of instruments was designed for a trio of trained musicians, who used more deliberate technique to perform the composed music. This paper outlines the hardware and sensor systems behind these devices: the electric field sensors of the Gesture Wall and Sensor Chair, the smart piezoelectric touchpads of the Rhythm Tree, the instrumented springs in Harmonic Driving, the pressure-sensitive touch screens of the Melody Easels, and the multimodal Digital Baton, containing a tactile interface, inertial sensors, and precise optical tracker. Also discussed are a set of controllers developed for the Brain Opera, but not currently touring with the production, including the Magic Carpet (immersive body sensing with a smart floor and Doppler radar) and an 8-channel MIDI-controlled sonar rangefinder.
Use of multiple biomarkers for a molecular diagnosis of prostate cancer (2004)

The identification of biomarkers capable of providing a reliable molecular diagnostic test for prostate cancer (PCa) is highly desirable clinically. We describe here 4 biomarkers, UDP-N-Acetyl-alpha-D-galactosamine transferase (GalNAc-T3; not previously associated with PCa), PSMA, Hepsin and DD3/PCA3, which, in combination, distinguish prostate cancer from benign prostate hyperplasia (BPH). GalNAc-T3 was identified as overexpressed in PCa tissues by microarray analysis, confirmed by quantitative real-time PCR and shown immunohistochemically to be localised to prostate epithelial cells with higher expression in malignant cells. Real-time quantitative PCR analysis across 21 PCa and 34 BPH tissues showed 4.6-fold overexpression of GalNAc-T3 (p = 0.005). The noncoding mRNA (DD3/PCA3) was overexpressed 140-fold (p = 0.007) in the cancer samples compared to BPH tissues. Hepsin was overexpressed 21-fold (p = 0.049, whereas the overexpression for PSMA was 66-fold (p = 0.047). When the gene expression data for these 4 biomarkers was combined in a logistic regression model, a predictive index was obtained that distinguished 100% of the PCa samples from all of the BPH samples. Therefore, combining these genes in a real-time PCR assay represents a powerful new approach to diagnosing PCa by molecular profiling. (Supplemental material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html.)
The role of hard coatings in carbide milling tools (1986)

The role of hard coatings in prolonging the life of cemented carbide tools is now well‐documented for continuous turning applications. In contrast, relatively few studies have elucidated the effect of such coatings in milling, where both mechanical and thermal cyclic loading impose special requirements on the tool cutting edge. This investigation describes the wear mechanisms observed in coated carbide milling tools which have been the object of recent commercial tool development. Coated carbide tools (by CVD and PVD) of varying cutting edge geometry, substrate, and coating composition were subjected to fly‐cutter milling tests under a nominal range of cutting conditions. Examination of the used cutting edges under optical and electron microscopy showed both thermal‐fatigue‐induced vertical cracks, perpendicular to the cutting edge, as well as lateral cracks parallel to the edge which result from mechanical cyclic stresses. The wear patterns and the sequential progression of these cracks were characterized on the used tools. It is noted that the coatings tend to inhibit crack propagation rather than reduce crack initiation and serve to delay the onset of typical failure mechanisms found in uncoated tools. The fracture toughness of the cutting edge is more critical in milling than in turning applications. A discussion is given on matching the requirements of edge geometry, material properties of the substrate, and the hard coating to optimize the performance of the coated carbide milling insert.
Antiischaemic effect of defibrotide treatment in rat kidney. (1993)

The authors previously demonstrated the protective activity of defibrotide (a profibrinolytic and antithrombotic drug) on endothelial cells. In the present work they examine the efficacy of defibrotide in protecting rat kidney from ischaemic/reperfusion injury by studying the modifications of intrarenal adenine nucleotide levels. Right renal ischaemia of 60 min and reperfusion of 30 min were induced in adult male Wistar rats. Defibrotide was administered as a bolus through a catheter inserted into the left femoral vein 5 min before the beginning of ischaemia at the dose of 32 mg/kg and continuously infused during ischaemia/reperfusion through the same vein at the final dose of 32 mg/kg in 5 ml of saline at the rate of 3 ml/h. Rats treated with vehicle of the drug were used as controls. At the end of postischaemic reperfusion, the ischaemic and left kidneys were rapidly removed and frozen in liquid nitrogen. Tissue extracts were prepared, and their ATP, ADP, AMP, cAMP, NAD+, and NADH contents were determined by using luminescence methods. In controls, ATP intrarenal levels were significantly higher in the left kidney than in the ischaemic organ of the same rat (3405 +/- 320 vs. 378 +/- 36 nmol/g fresh tissue and mean +/- s.e.m. of 10 experiments). Defibrotide treatment significantly protected ischaemic kidneys from the drop in ATP intrarenal content (1465 +/- 147 vs. 3124 +/- 303 nmol/g fresh tissue measured in the left kidney).
Antibiotics in Early Life Alter the Gut Microbiome and Increase Disease Incidence in a Spontaneous Mouse Model of Autoimmune Insulin-Dependent Diabetes (2015)

Insulin-dependent or type 1 diabetes is a prototypic autoimmune disease whose incidence steadily increased over the past decades in industrialized countries. Recent evidence suggests the importance of the gut microbiota to explain this trend. Here, non-obese diabetic (NOD) mice that spontaneously develop autoimmune type 1 diabetes were treated with different antibiotics to explore the influence of a targeted intestinal dysbiosis in the progression of the disease. A mixture of wide spectrum antibiotics (i.e. streptomycin, colistin and ampicillin) or vancomycin alone were administered orally from the moment of conception, treating breeding pairs, and during the postnatal and adult life until the end of follow-up at 40 weeks. Diabetes incidence significantly and similarly increased in male mice following treatment with these two antibiotic regimens. In NOD females a slight yet not significant trend towards an increase in disease incidence was observed. Changes in gut microbiota composition were assessed by sequencing the V3 region of bacterial 16S rRNA genes. Administration of the antibiotic mixture resulted in near complete ablation of the gut microbiota. Vancomycin treatment led to increased Escherichia, Lactobacillus and Sutterella genera and decreased members of the Clostridiales order and Lachnospiraceae, Prevotellaceae and Rikenellaceae families, as compared to control mice. Massive elimination of IL-17-producing cells, both CD4+TCRαβ+ and TCRγδ+ T cells was observed in the lamina propria of the ileum and the colon of vancomycin-treated mice. These results show that a directed even partial ablation of the gut microbiota, as induced by vancomycin, significantly increases type 1 diabetes incidence in male NOD mice thus prompting for caution in the use of antibiotics in pregnant women and newborns.
Topical diclofenac epolamine patch 1.3% for treatment of acute pain caused by soft tissue injury (2010)

Acute pain caused by musculoskeletal disorders is very common and has a significant negative impact on quality-of-life and societal costs. Many types of acute pain have been managed with traditional oral non-steroidal anti-inflammatory drugs (NSAIDs) and selective cyclooxygenase-2 inhibitors (coxibs). Data from prospective, randomised controlled clinical trials and postmarketing surveillance indicate that use of oral traditional NSAIDs and coxibs is associated with an elevated risk of developing gastrointestinal, renovascular and/or cardiovascular adverse events (AEs). Increasing awareness of the AEs associated with NSAID therapy, including coxibs, has led many physicians and patients to reconsider use of these drugs and look for alternative treatment options. Treatment with NSAIDs via the topical route of administration has been shown to provide clinically effective analgesia at the site of application while minimising systemic absorption. The anti-inflammatory and analgesic potency of the traditional oral NSAID diclofenac, along with its physicochemical properties, makes it well suited for topical delivery. Several topical formulations of diclofenac have been developed. A topical patch containing diclofenac epolamine 1.3% (DETP, FLECTOR(®) Patch), approved for use in Europe in 1993, has recently been approved for use in the United States and is indicated for the treatment of acute pain caused by minor strains, sprains and contusions. In this article, we review the available clinical trial data for this product in the treatment of pain caused by soft tissue injury.
Three-Dimensional Blood Vessel Quantification via Centerline Deformation (2008)

It is clinically important to quantify the geometric parameters of an abnormal vessel, as this information can aid radiologists in choosing appropriate treatments or apparatuses. Centerline and cross-sectional diameters are commonly used to characterize the morphology of vessel in various clinical applications. Due to the existence of stenosis or aneurysm, the associated vessel centerline is unable to truly portray the original, healthy vessel shape and may result in inaccurate quantitative measurement. To remedy such a problem, a novel method using an active tube model is proposed. In the method, a smoothened centerline is determined as the axis of a deformable tube model that is registered onto the vessel lumen. Three types of regions, normal, stenotic, and aneurysmal regions, are defined to classify the vessel segment under-analyzed by use of the algorithm of a cross-sectional-based distance field. The registration process used on the tube model is governed by different region-adaptive energy functionals associated with the classified vessel regions. The proposed algorithm is validated on the 3-D computer-generated phantoms and 3-D rotational digital subtraction angiography (DSA) datasets. Experimental results show that the deformed centerline provides better vessel quantification results compared with the original centerline. It is also shown that the registered model is useful for measuring the volume of aneurysmal regions.
Telomerase activity associated with acquisition of malignancy in human colorectal cancer. (1995)

Shortening of telomeres may contribute to the control of the proliferative capacity of normal cells, and telomerase, the enzyme that elongates telomeric DNA, may be essential for unlimited cell proliferation. We have shown previously that telomerase activity is present in human cells immortalized in vitro and in metastatic ovarian carcinoma cells but is undetectable in normal cultured cells or normal tissues. We have determined the temporal pattern of telomerase activity during colorectal carcinogenesis in man. We report that telomerase activity is associated with acquisition of malignancy as it is detectable in colorectal carcinoma but not in adenomatous polyps. Mutations leading to reactivation or upregulation of the enzyme may represent an additional required event in the multistep development of colorectal cancer.
Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods. (1989)

We developed an immunohistochemical method for visualization of vitamin D (VDR) and estrogen receptors (ER) in cryostat sections, using monoclonal antibodies (MAb) to the vitamin D receptor and estrogen receptor, respectively. This method is based on an avidin-biotin labeling technique (LAB). To establish a reliable and sensitive method which can be used easily as a routine diagnostic procedure, we systematically compared four different immunoenzymatic methods with respect to their efficiency in detecting vitamin D and estrogen receptors. Compared to the indirect bridged avidin-biotin (IBRAB), the peroxidase- anti-peroxidase (PAP), and the avidin-biotin complex (ABC) methods, the LAB method produced stronger staining intensities and had higher detection efficiency for both vitamin D and estrogen receptors. In addition, the LAB method had a higher spatial resolution compared to the ABC technique in detection of VDR in normal human skin biopsies. In the case of steroid receptors, i.e., nuclear antigens, immunohistochemistry must deal with a relatively low number of antigenic sites per cell, restricted accessibility of the antigens, and slight differences in antigen concentrations among cells. Under these particular conditions, the chemical properties of the conjugates used in the LAB method may explain why it is superior to the other methods. Consequently, the LAB method is recommended for visualization of ER and VDR.
Preventing ischemic stroke (2000)

Preventing stroke is the most important strategy for reducing the cost of this disease. Management of modifiable risk factors, especially hypertension and Oral anticoagulation with warfarin for selected high-risk patients with nonvalvular atrial fibrillation. Carotid endarterectomy for selected patients with carotid artery stenosis greater than 60%. Regular physical exercise. Treatment with statin medications for patients who have coronary artery disease with or without hyperlipidemia. Routine use of antiplatelet medication has no proven role in primary stroke prevention, although aspirin is often prescribed for patients with vascular risk factors who have not yet had symptoms of either stroke or ischemic heart disease. The major strategies for secondary stroke prevention are: Appropriate evaluation to identify the mechanism of the initial stroke. Carotid endarterectomy for patients with symptomatic carotid artery stenosis of 50% or more. Oral anticoagulation with warfarin for patients with nonvalvular atrial fibrillation. Use of various antiplatelet agents, including aspirin, ticlopidine, clopidogrel, and the combination of aspirin and slow-release dipyridamole. Whether treatment of risk factors reduces the risk of secondary stroke is currently being evaluated in clinical trials.
Temperatures Induced by High Power Lasers: Effects on Reservoir Rock Strength and Mechanical Properties (2002)

Abstract The temperature effects of high power lasers on rock strength and mechanical properties are presented. It was found that the high temperature induced by lasers drilling through rock enhances porosity and permeability and reduces strength. High temperature causes microcracks, vaporizes cementation, and dehydrates clays. Microcracks were monitored using thin sections and SEM analyses. Representative samples were cut for thin section analyses before and after lasing. For the SEM test, a small fragment of the sample was removed and coated with gold for better electron conductivity, thus allowing fracture imaging in three dimensions. Phase changes also affect rock properties such as permeability, porosity and strength. The effect depends on the temperature distribution and gradient as well as the mineralogy. At each temperature, different reactions take place. For example, at 550°C smectite clay will collapse and dehydrate. This mechanism results in increasing void space in the rock sample. At the same temperature, quartz grains will expand by 1.75%1 and when the grains cool, microcracks are created, which also increases the void space. This increase in void space may or may not change permeability, but it does decrease the acoustic velocities that in turn change the calculated porosity and elastic moduli. Results show in all cases, the Young's modulus, shear modulus, bulk modulus, and combined modulus near the lased portion, were reduced when compared to the unlased portion. In sandstones, the decrease was 50% or more. A bulk modulus decreased of 50% causes the compressibility near the wellbore to increase by 50%. Depending on the lithology, Poisson's ratio decreased or increased slightly. For limestones and shales, the differences between the moduli of lased and unlased samples were less significant than in sandstone.
Nanocubes and Nanoboxes (2002)

Upcycled synthesis and extraction of carbon‐encapsulated iron carbide nanoparticles for gap Plasmon applications in perovskite solar cells,
Robust Acoustic Time Reversal with High-Order Multiple Scattering (1995)

We report the first experiments showing the reversibility of transient acoustic waves through high-order multiple scattering by means of an acoustic time-reversal mirror. A point source generates a pulse which scatters through 2000 steel rods immersed in water. The time-reversed waves are found to converge to their source and recover their original wave form, despite the high order of multiple scattering involved and the usual sensitivity to initial conditions of time-reversal processes. Surprisingly, the observed resolution was one-sixth of the theoretical limit for the mirror's aperture.
Demonstration of low resistance ohmic contacts to p-type (2021) GaN (2015)

We demonstrate low resistance Ohmic contacts to ( semipolar p-type GaN using a thin p++–GaN contact layer. This layer was optimized by varying the V/III ratio, Cp2Mg flow, thickness, and growth rate which produced low forward voltage devices with specific contact resistivities estimated to be 4 × 10−4 Ω cm2 and 5 × 10−4 Ω cm2 for palladium contacts and indium tin oxide (ITO) contacts respectively. Forward voltages of 2.89 V and 3.47 V were obtained at 20 A cm−2 and 800 A cm−2 respectively for light emitting diodes (LEDs) emitting at 435 nm with palladium contacts. LEDs with ITO contacts had forward voltages of 2.94 V and 3.55 V at 20 A cm−2 and 800 A cm−2 respectively.
Cloning and nucleotide sequence of the trxA gene of Escherichia coli K-12 (1985)

The Escherichia coli K-12 trxA gene, the gene encoding thioredoxin, has been cloned and sequenced. The DNA sequence includes 280 base pairs upstream and 46 base pairs downstream of the coding region. The downstream sequence contains the -35 region of the promoter of the rho gene. Northern analysis of the trxA mRNA and S1 nuclease mapping indicate the presence of two promoters for the trxA gene. Initiation from either promoter results in an mRNA containing two potential translation initiation codons, one of which could initiate synthesis of a protein 18 amino acids longer than the mature trxA gene product. The 3' end of the gene, including the last eight codons, contains a stable stem-loop structure (delta G = -12.9 kcal) typical of a rho-independent transcription termination signal.