gebhart/patrepeval huggingface dataset:
IgE-mediated release of leukotriene C4, chondroitin sulfate E proteoglycan, beta-hexosaminidase, and histamine from cultured bone marrow-derived mouse mast cells. (1983)

Mouse bone marrow-derived mast cells differentiated in vitro and sensitized with monoclonal IgE respond to antigen-initiated activation with the release of histamine, beta-hexosaminidase, chondroitin sulfate E proteoglycan, and leukotriene C4 (LTC4). The chondroitin sulfate E nature of the glycosaminoglycan side chain was established by demonstrating that the chondroitinase ABC disaccharide digestion products were composed of equal quantities of 4-sulfated and 4,6-disulfated N-acetyl-galactosamine. The single immunoreactive sulfidopeptide leukotriene, released and quantitated with a class-specific antibody, was identified as LTC4 by its retention time on reverse-phase high-performance liquid chromatography and by its specific spasmogenic activity on the guinea pig ileum. The release of the preformed mediators, as well as of LTC4, was related in a dose-response fashion to the concentration of monoclonal IgE used during the sensitization step and to the concentration of specific antigen used to initiate the activation-secretion response. The optimal concentrations of IgE for sensitization and of antigen for challenge were the same for the release of preformed mediators and of LTC4. In addition, the time courses of their release were superimposable, with a plateau at 5 min after antigen challenge. The release of three preformed mediators and of LTC4 after fixation of IgE, washing of the sensitized cells, and antigen challenge unequivocally indicates a bone marrow-derived mast cell origin for these products. Linear regression analyses of the net percent release of beta-hexosaminidase to histamine and of 35S-chondroitin sulfate E to beta-hexosaminidase yielded straight lines that intersected at the origin, which indicates that the three preformed mediators are localized in the secretory granules of the bone marrow-derived mast cells. The concomitant generation of 23 ng of LTC4/10(6) sensitized bone marrow-derived mast cells represents the first example of IgE-dependent release of substantial amounts of LTC4, a component of slow reacting substance of anaphylaxis, from a mast cell population of greater than 95% purity. The IgE-dependent generation of LTC4, rather than prostaglandin D2, by the chondroitin sulfate E proteoglycan-containing bone marrow-derived mast cells contrasts with the predominant generation of prostaglandin D2 by heparin proteoglycan-containing mast cells. These differences together support the existence of two phenotypically different mast cell subclasses.
Production of L- methionine by Bacillus cereus isolated from different soil ecovars in Owerri, South East Nigeria (2012)

A total of 500 bacteria isolated from 20 different soil ecovars were screened for methionine production, three isolates designated as DS-13, AS-9 and RS-16 were recovered. Taxonomic studies revealed that all strains showed good growth and sporulation on most media, utilized various sugars, reduced citrate and was negative for nitrate and urease. Molecular characterization conducted showed that the isolates were different strains of Bacillus cereus. Bacillus cereus RS 16 produced the highest quantity of 1.84mg/ml after 96 hours while Bacillus cereus AS-9 produced the least quantity of 1.21mg/ml after 96 hours.
Experiments on multistrategy learning by meta-learning (1993)

In this paper, we propose meta-leaming as a general technique to combine the results of multiple learning algorithms,
Mining biomarkers in human sera using proteomic tools (2004)

One of the major difficulties in mining low abundance biomarkers from serum or plasma is due to the fact that a small number of proteins such as albumin, alpha2-macroglobulin, transferrin, and immunoglobulins, may represent as much as 80% of the total serum protein. The large quantity of these proteins makes it difficult to identify low abundance proteins in serum using traditional 2-dimensional electrophoresis. We recently used a combination of multidimensional liquid chromatography and gel electrophoresis coupled to matrix-assisted laser desorption/ionization-quadrupole-time of flight and Ion Trap liquid chromatography-tandem mass spectrometry to identify protein markers in sera of Alzheimer's disease (AD), insulin resistance/type-2 diabetes (IR/D2), and congestive heart failure (CHF) patients. We identified 8 proteins that exhibit higher levels in control sera and 36 proteins that exhibit higher levels in disease sera. For example, haptoglobin and hemoglobin are elevated in sera of AD, IR/D2, and CHF patients. The levels of several other proteins including fibrinogen and its fragments, alpha 2-macroglobulin, transthyretin, pro-platelet basic protein, protease inhibitors clade A and C, as well as proteins involved in the classical complement pathway such as complement C3, C4, and C1 inhibitor, were found to differ between IR/D2 and control sera. The sera levels of proteins, such as the 10 kDa subunit of vitronectin, alpha 1-acid glycoprotein, apolipoprotein B100, fragment of factor H, and histidine-rich glycoprotein were observed to be different between AD and controls. The differences observed in these biomarker candidates were confirmed by Western blot and the enzyme-linked immunosorbent assay. The biological meaning of the proteomic changes in the disease states and the potential use of these changes as diagnostic tools or for therapeutic intervention will be discussed.
The impact of transcranial magnetic stimulation on cognitive processing: an event-related potential study (2001)

Several neuropsychological studies have shown that repetitive transcranial magnetic stimulation (rTMS) can improve cognitive processing. We performed a study on the impact of rTMS on cognitive processing as measured by a neurophysiological method. In 14 healthy subjects, visually evoked event-related potentials (ERP) and mean choice reaction time were measured before and after 20 Hz rTMS of the left and of the right prefrontal cortex. The data were compared to sham stimulation and to 1 Hz single TMS. P3 latencies and reaction time were significantly decreased by rTMS of the left but not of the right prefrontal cortex, single TMS did not have any significant impact on the ERP components. We conclude that the facilitating effects of rTMS on cognitive processing can be proven even by objective neurophysiological measures.
Rapid Microscale Synthesis, a New Method for Lead Optimization Using Robotics and Solution Phase Chemistry:  Application to the Synthesis and Optimization of Corticotropin-Releasing Factor1Receptor Antagonists (1996)

ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTRapid Microscale Synthesis, a New Method for Lead Optimization Using Robotics and Solution Phase Chemistry: Application to the Synthesis and Optimization of Corticotropin-Releasing Factor1 Receptor AntagonistsJeffrey P. Whitten, Yun Feng Xie, Philip E. Erickson, Thomas R. Webb, Errol B. De Souza, Dimitri E. Grigoriadis, and James R. McCarthyView Author Information Neurocrine Biosciences, 3050 Science Park Road, San Diego, California 92121 Cite this: J. Med. Chem. 1996, 39, 22, 4354–4357Publication Date (Web):October 25, 1996Publication History Received20 May 1996Published online25 October 1996Published inissue 1 January 1996https://doi.org/10.1021/jm960148mCopyright © 1996 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views442Altmetric-Citations55LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (181 KB) Get e-AlertsSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Antagonists,Chemical synthesis,Receptors,Solution phase,Triazine Get e-Alerts
Improved oligonudeotide site-directed rautagenesis using M13 vectors (1985)

An improved method is described for the construction of mutations in M13 vectors using synthetic oligonucleotides. The DNA is first cloned into a novel M13 vector (based upon M13mp18 or M13mp19), which carries a genetic marker that can be selected against, such as an EcoK or EcoB site, or an amber mutation in an essential phage gene. In this "coupled priming" technique, one primer is used to construct the silent mutation of interest, and a second primer is used to eliminate the selectable marker on the minus strand. After primer extension and ligation, the heteroduplex DNA is transfected into a strain of E. coli which is repair deficient and selects against the plus strand marker. Over 50 mutants have been constructed with this approach, and the yields can be excellent (up to 70%). For the stepwise construction of mutations using separate rounds of mutagenesis, the EcoK and EcoB markers offer a particular advantage over the amber marker. They permit selection in each round, as it is possible to cycle between the two markers. However for construction of multiple mutations over a short region, long synthetic oligonucleotides with multiple mismatches to the template can offer an alternative strategy.
Will pollen-mediated gene flow from industrial Eucalyptus plantations impact on the genetic integrity of native eucalypt forests in Australia? (2008)

With the large increase in industrial eucalypt plantations in Australia over the last decade, there is growing concern that native eucalypt gene pools may be contaminated by pollen flow from locally exotic eucalypt species or provenances. We are studying the risk of hybridisation from the two most commonly planted hardwood species in industrial plantations in Australia, Eucalyptus nitens and E. globulus. While both species are native to Australia, they are frequently planted outside their natural range and in close proximity to species with which they do not co-occur naturally. While hybridisation will not occur when adjacent species belong to different subgenera to the plantation species, barriers to hybridisation within subgenera of Eucalyptus are frequently weak and exotic gene flow is then possible. A number of steps in the process of pollen-mediated gene flow have therefore been assessed. Firstly, field surveys across the entire temperate hardwood estate have confirmed that many compatible native eucalypts are found adjacent to plantations and that most older plantations are reproductively active. Secondly, open-pollinated seed collections have demonstrated that plantations can hybridise with some native species (most notably E. camaldulensis and E. ovata) and exotic F1 hybrid seedlings involving native E. ovata have been identified in the wild. Thirdly, artificial pollination studies have confirmed that numerous other hybrid combinations are possible involving species from the same subgenus as E. globulus and E. nitens. Fourthly, there is evidence to suggest that flowering time asynchrony and spatial isolation are important barriers which will reduce or prevent hybridisation in many cases. Fifthly, despite evidence that first generation hybrids are often less fit than parental species, some hybrids may survive to reproduction and backcross with native species. Full assessment of the impact of such hybridisation will depend on long-term studies of hybrid fitness. Strategies to identify species most at risk of exotic gene flow have been developed and practical measures to minimise this risk have been identified.
Cost-effectiveness of 24 or 48 weeks of interferon α-2b alone or with ribavirin as initial treatment of chronic hepatitis c (2000)

OBJECTIVE: Initial therapy with ribavirin and interferon α-2b results in a higher sustained virological response than interferon alone, but this regimen is expensive. We aimed to examine the cost-effectiveness of 24- or 48-wk initial treatment with combination therapy versus interferon alone for patients who have chronic hepatitis C. METHODS: Data from recent randomized clinical trials comparing combination therapy to interferon alone were applied to a previously published computer cohort simulation to project lifelong clinical and economic outcomes. Natural history and economic estimates were based on published literature, expert panel estimates, and actual variable cost and reimbursement data. RESULTS: Using treatment stopping rules, sustained viral negative response rates would be 33.1% and 39.8% for patients receiving 24 versus 48 wk of ribavirin/interferon, compared with 14.3% for 48 wk of interferon alone. Compared to the interferon alone strategy, 24 or 48 wk of combination therapy should prolong life expectancy by 1.4 to 2.0 yr at marginal cost-effectiveness ratios of $4400 to $5400 per discounted quality-adjusted life-year (DQALY) gained. Compared to 24 wk of combination therapy, 48 wk of combination therapy should prolong life expectancy by 0.6 yr at a marginal cost-effectiveness ratio of $7700 per DQALY gained. The results were robust, with 24 or 48 wk of combination therapy remaining preferred and cost-effective in sensitivity analysis compared with interferon alone. CONCLUSION: For patients with chronic hepatitis C, 24 or 48 wk of ribavirin and interferon should prolong life and be cost-effective when compared with 48 wk of interferon alone.
Systemic Efficacy with Oncolytic Virus Therapeutics: Clinical Proof-of-Concept and Future Directions (2007)

Oncolytic viruses that can destroy cancer cells have been engineered in a variety of ways with the aim of improving their selectivity and efficacy. Here, we review data from clinical investigations of these virotherapeutic agents, specifically those that have shown systemic efficacy: vaccinia, measles, mumps, viruses, Newcastle disease virus, and reovirus. Further directions for optimizing i.v. delivery and efficacy are discussed.
Targets for Cell Cycle Arrest by the Immunosuppressant Rapamycin in Yeast (1991)

FK506 and rapamycin are related immunosuppressive compounds that block helper T cell activation by interfering with signal transduction. In vitro, both drugs bind and inhibit the FK506-binding protein (FKBP) proline rotamase. Saccharomyces cerevisiae cells treated with rapamycin irreversibly arrested in the G1 phase of the cell cycle. An FKBP-rapamycin complex is concluded to be the toxic agent because (i) strains that lack FKBP proline rotamase, encoded by FPR1, were viable and fully resistant to rapamycin and (ii) FK506 antagonized rapamycin toxicity in vivo. Mutations that conferred rapamycin resistance altered conserved residues in FKBP that are critical for drug binding. Two genes other than FPR1, named TOR1 and TOR2, that participate in rapamycin toxicity were identified. Nonallelic noncomplementation between FPR1, TOR1, and TOR2 alleles suggests that the products of these genes may interact as subunits of a protein complex. Such a complex may mediate nuclear entry of signals required for progression through the cell cycle.
Complete nucleotide sequence of the xylanase gene from the yeastCryptococcus albidus (1988)

Journal Article Complete nucleotide sequence of the xylanase gene from the yeast Cryptococcus albidus Get access Francois Boucher, Francois Boucher Centre de Recherche en Microbiologie Appliquée, Institut Armand-FrappierVille de Laval, Québec H7V IB7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Rolf Morosoli, Rolf Morosoli * Centre de Recherche en Microbiologie Appliquée, Institut Armand-FrappierVille de Laval, Québec H7V IB7, Canada * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Serge Durand Serge Durand Centre de Recherche en Microbiologie Appliquée, Institut Armand-FrappierVille de Laval, Québec H7V IB7, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 20, 25 October 1988, Page 9874, https://doi.org/10.1093/nar/16.20.9874 Published: 25 October 1988 Article history Received: 15 August 1988 Published: 25 October 1988
[Cardiac output evaluation during exercise in children treated with atrial surgery for transposition of great vessels]. (1996)

The long-term physiopathological consequences of atrial surgery (Senning or Mustard procedures) for transposition of the great vessels with respect to exercise capacity are not well known. We measured the cardiac index by the technique of CO2 rebreathing at two submaximal levels of exercise corresponding to a stable oxygen consumption of 20 (E20) and 30 (E30) ml/min/kg in 7 patients successfully operated for transposition of the great vessels and in 7 control children paired for age, gender and body surface area. Despite an identical chronotropic response to exercise in the two groups, the increase in cardiac index was not as great in the children operated for transposition (from 6.86 +/- 0.51 to 7.71 +/- 0.78 l/min/m2) as in the control population (from 7.71 +/- 0.78 to 10.2 +/- 0.51 l/min/m2; p < 0.02). The stroke volume index was therefore significantly lower in the transposition group at both levels of exercise (52 +/- 3.2 vs 63 +/- 4.1 ml/m2; p < 0.04 at E20; and 46.4 +/- 4.3 vs 66 +/- 5.1 ml/m2 at E30). The main cause of this reduction of the stroke volume index is probably a lack of adaptation of right ventricular systolic function on exercise but it is not possible to exclude diastolic dysfunction due to reduce compliance secondary to the intraatrial patch. The conditions of preload are in fact instrumental in increasing stroke volume index at submaximal exercise levels.
Fabrication of Hollow‐Fiber Membranes by Phase Inversion (2008)

This chapter contains sections titled: Introduction Basic Understanding Recent Progresses on Single-Layer Asymmetric Hollow-Fiber Membranes Dual-Layer Hollow Fibers Concluding Remarks Acknowledgments References
Characterization of a region in mature LamB protein that interacts with a component of the export machinery of Escherichia coli. (1990)

It has been shown that the synthesis of an export-defective protein can interfere with the normal export process in Escherichia coli by limiting the availability of SecB protein, a component of the export apparatus (Collier, D.N., Bankaitis, V.A., Weiss, J.B., and Bassford, P.J. (1988) Cell 53, 273-283). Consistent with this observation, we find that the interference elicited by an export-defective LamB protein is a titratable response resulting from the limitation of a single ligand. We have mapped the interfering region in LamB to between amino acids 320 and 380 of the mature protein. Expression of this sequence in the form of a LacZ-LamB-LacZ fusion protein elicits the export interference phenotype. Deletion of the sequence from an export-defective LamB protein eliminates the ability of this protein to interfere with the export of other secreted proteins. Together, these findings show that this sequence is both necessary and sufficient to cause export interference. Surprisingly, deletion of this sequence from an otherwise wild-type LamB protein does not cause the mutant LamB product to exhibit any obvious export defect. Based on our results, we propose that SecB interacts with both amino acids 320-380 of mature LamB and the LamB signal sequence during initiation of the export process.
Terlipressin-Ephedrine Versus Ephedrine to Treat Hypotension at the Induction of Anesthesia in Patients Chronically Treated with Angiotensin Converting-Enzyme Inhibitors: A Prospective, Randomized, Double-Blinded, Crossover Study (2002)

Vascular surgical patients chronically treated with drugs that inhibit the functioning of the renin-angiotensin system may experience hypotension unresponsive to conventional therapy. This double-blinded, cross-over study demonstrated that in these patients the use of a vasopressin analog, terlipressin given with ephedrine, was effective in reversing intraoperative systemic hypotension refractory to ephedrine.
Deoxyribonucleic acid vaccines encoding antigens with rapid proteasome-dependent degradation are highly efficient inducers of cytolytic T lymphocytes (1997)

We generated plasmid expression vectors encoding ubiquitin and beta-galactosidase (beta-gal) with different intervening amino acids, allowing for the production of processed protein products that have either stabilizing or destabilizing residues at their N-termini. P815 cells transfected with plasmids encoding beta-gal with a destabilizing N-terminus did not have detectable expression beta-gal unless they were treated with inhibitors specific for the proteasome. Inhibitors of other proteolysis pathways had no such effect. Nevertheless, transfectants expressing beta-gal with different amino acid residues were equally sensitive to cytolysis by a CTL clone specific for a beta-gal peptide presented in the context of H-2Ld. In contrast to vectors encoding native beta-gal, plasmid vectors encoding beta-gal with a destabilizing residue did not induce detectable anti-beta-gal Abs when injected into skeletal muscle of BALB/c mice. However, such vectors were significantly more effective than vectors encoding native beta-gal or beta-gal with a stabilizing residue in stimulating CTL specific for P13.2, a lacZ transfectant of P815. We conclude that incorporation of strategies that enhance proteasome-dependent degradation may generate DNA vaccines that are more effective in inducing cellular immunity against targeted Ags.
Switchable information carriers based on shape memory polymer (2012)

Herein we demonstrate the realization of a new technological concept, which enables the use of shape memory polymers (SMPs) as switchable information carriers. At first, we applied a surface-specific dyeing process based on ‘guest-diffusion’ on two sophisticated polymeric host materials, including a thermoplastic poly(ester urethane) SMP and a thermoset epoxy-based SMP. Upon drying, self-assembly of the dye molecules inside the polymer surfaces occurred, resulting in homogeneous color penetration depths of about 100 μm. Subsequently, the colored surfaces were patterned with quick response (QR) codes. For this purpose, laser ablation was used. The resulting cavity depth was exceeding the color penetration depth. This assured sufficient surface contrast and rendered the QR codes machine-readable. In a progressive approach, two thermo-mechanical functionalization protocols were designed in accordance with the thermal properties of the polymers. As a result of programming, the tag prototypes were converted into stable, temporary shapes with non-decodable QR code information. When thermally triggering the shape memory effect on the functionalized tags, we verified the mostly complete recovery of the polymer surface and the associated restoration into the almost original shape. As such, the QR code could again precisely be read out. We anticipate that tagging products with these information carriers is helpful for the purpose of secure one-time identification.