gebhart/patrepeval huggingface dataset:
Resolution enhancement of monochrome and color video using motion compensation (2001)

We propose an iterative algorithm for enhancing the resolution of monochrome and color image sequences. Various approaches toward motion estimation are investigated and compared. Improving the spatial resolution of an image sequence critically depends upon the accuracy of the motion estimator. The problem is complicated by the fact that the motion field is prone to significant errors since the original high-resolution images are not available. Improved motion estimates may be obtained by using a more robust and accurate motion estimator, such as a pel-recursive scheme instead of block matching, in processing color image sequences, there is the added advantage of having more flexibility in how the final motion estimates are obtained, and further improvement in the accuracy of the motion field is therefore possible. This is because there are three different intensity fields (channels) conveying the same motion information. In this paper, the choice of which motion estimator to use versus how the final estimates are obtained is weighed to see which issue is more critical in improving the estimated high-resolution sequences. Toward this end, an iterative algorithm is proposed, and two sets of experiments are presented. First, several different experiments using the same motion estimator but three different data fusion approaches to merge the individual motion fields were performed. Second, estimated high-resolution images using the block matching estimator were compared to those obtained by employing a pel-recursive scheme. Experiments were performed on a real color image sequence, and performance was measured by the peak signal to noise ratio (PSNR).
Signaling through P2X7 Receptor in Human T Cells Involves p56 , MAP Kinases, and Transcription Factors AP-1 and NF-κB (2003)

ATP-gated ion channel P2X receptors are expressed on the surface of most immune cells and can trigger multiple cellular responses, such as membrane permeabilization, cytokine production, and cell proliferation or apoptosis. Despite broad distribution and pleiotropic activities, signaling pathways downstream of these ionotropic receptors are still poorly understood. Here, we describe intracellular signaling events in Jurkat cells treated with millimolar concentrations of extracellular ATP. Within minutes, ATP treatment resulted in the phosphorylation and activation of p56(lck) kinase, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase but not p38 kinase. These effects were wholly dependent upon the presence of extracellular Ca(2+) ions in the culture medium. Nevertheless, calmodulin antagonist calmidazolium and CaM kinase inhibitor KN-93 both had no effect on the activation of p56(lck) and ERK, whereas a pretreatment of Jurkat cells with MAP kinase kinase inhibitor P098059 was able to abrogate phosphorylation of ERK. Further, expression of c-Jun and c-Fos proteins and activator protein (AP-1) DNA binding activity were enhanced in a time-dependent manner. In contrast, DNA binding activity of NF-kappa B was reduced. ATP failed to stimulate the phosphorylation of ERK and c-Jun N-terminal kinase and activation of AP-1 in the p56(lck)-deficient isogenic T cell line JCaM1, suggesting a critical role for p56(lck) kinase in downstream signaling. Regarding the biological significance of the ATP-induced signaling events we show that although extracellular ATP was able to stimulate proliferation of both Jurkat and JCaM1 cells, an increase in interleukin-2 transcription was observed only in Jurkat cells. The nucleotide selectivity and pharmacological profile data supported the evidence that the ATP-induced effects in Jurkat cells were mediated through the P2X7 receptor. Taken together, these results demonstrate the ability of extracellular ATP to activate multiple downstream signaling events in a human T-lymphoblastoid cell line.
Characterization of dispersive surface waves using continuous wavelet transforms (2005)

In this paper, we propose a method of surface waves characterization based on the deformation of the wavelet transform of the analysed signal. An estimate of the phase velocity (the group velocity) and the attenuation coefficient is carried out using a model-based approach to determine the propagation operator in the wavelet domain, which depends nonlinearly on a set of unknown parameters. These parameters explicitly define the phase velocity, the group velocity and the attenuation. Under the assumption that the difference between waveforms observed at a couple of stations is solely due to the dispersion characteristics and the intrinsic attenuation of the medium, we then seek to find the set of unknown parameters of this model. Finding the model parameters turns out to be that of an optimization problem, which is solved through the minimization of an appropriately defined cost function. We show that, unlike time-frequency methods that exploit only the square modulus of the transform, we can achieve a complete characterization of surface waves in a dispersive and attenuating medium. Using both synthetic examples and experimental data, we also show that it is in principle possible to separate different modes in both the time domain and the frequency domain
Kinetic characterization of the carbon monoxide-acetyl-CoA (carbonyl group) exchange activity of the acetyl-CoA synthesizing carbon monoxide dehydrogenase from Clostridium thermoaceticum (1988)

CO dehydrogenase from Clostridium thermoaceticum is a nickel-containing enzyme that catalyzes both the reversible conversion of CO2 to CO (for incorporation into the carbonyl group of acetate) and the synthesis of acetyl-CoA from methyl corrinoid, CO, and CoASH. The latter activity is conveniently assayed by monitoring the exchange of [1-14C]acetyl-CoA (carbonyl group) with 12CO. Kinetic parameters for the highly oxygen sensitive exchange activity have been determined: Km (acetyl-CoA) = 600 microM; Vmax = 440 min-1. In addition, coenzyme A analogues have been tested as inhibitors of the exchange to probe the active site of the enzyme; each has no effect on the CO2 in equilibrium CO activity of CO dehydrogenase. Coenzyme A, the substrate for acetate biosynthesis, is a potent competitive inhibitor, KI = 7 microM. Comparison of this value with that for desulfo-CoA (KI = 6000 microM) suggests that a key mode of binding is through the sulfur atom, possibly to a metal site on the enzyme. The relatively high affinity of the enzyme for CoASH relative to acetyl-CoA is consistent with its proposed operation in the acetogenic direction. The differential sensitivity to oxygen and storage of the two activities of CO dehydrogenase as well as the contrasting effect of coenzyme A inhibitors suggests that acetate assemblage occurs at a site distinct from that for CO dehydrogenation.
Efficacy of intrarectal lidocaine hydrochloride gel for pain control in patients undergoing transrectal prostate biopsy (2004)

We concluded that lidocaine probably exerts a minimal effect on patients' tolerance to pain on transrectal prostate biopsy.
Surface micromachined accelerometers (1996)

Surface micromachining has enabled the cofabrication of thin-film micromechanical structures and CMOS or bipolar/MOS integrated circuits. Using linear, single-axis accelerometers as a motivating example, this paper discusses the fundamental mechanical as well as the electronic noise floors for representative capacitive position-sensing interface circuits. Operation in vacuum lowers the Brownian noise of a polysilicon accelerometer to below 1 /spl mu/g//spl radic/(Hz). For improved sensor performance, the position of the microstructure should be controlled using electrostatic force-feedback. Both analog and digital closed-loop accelerometers are described and contrasted, with the latter using high-frequency voltage pulses to apply force quanta to the microstructure and achieve a very linear response.
An approach to multi-copy search in molecular replacement (2000)

The molecular-replacement method has been extended to a simultaneous search for multiple copies of the macromolecule in the unit cell. The central point of this approach is the construction of a multi-copy search model from the properly oriented monomers using a special translation function. The multi-copy search method has been implemented in the program MOLREP and successfully tested using experimental data.
Bell Telephone Laboratories, Incorporated (1972)

All published accounts of the use of Bond's method for lattice parameter measurements have used step-scanning (at equal angle increments) of the diffraction peaks, followed by graphical or computer analysis of the data to locate the peak positions. It has been found that the peak angles can be determined with little loss in accuracy or precision by manually setting the crystal angle to give a counting rate (observed on a rate-meter) equal to 1/2 the peak rate and defining the peak angle as the average of the angles on the two sides of the peak. Because of the asymmetry of the spectral line, defining the peak in this manner results in a shift of the peak angle from that determined by the mid-chord peak method. This shift can be compensated by determining an effective value of the wavelength based on a silicon standard. Using the method de­ scribed, a lattice parameter measurement, including mounting and orienting the sample, taking the data and computing the result using a time-sharing computer terminal, can be made in less than 20 mins.
Humanin rescues human cerebrovascular smooth muscle cells from Aβ‐induced toxicity (2003)

Abstract Cerebral amyloid β‐protein (Aβ) angiopathy (CAA) is a key pathological feature of Alzheimer's disease (AD) and related disorders. We have used human cerebrovascular smooth muscle (HCSM) cells as an in vitro model system to investigate the pathogenic mechanisms of the pathology of CAA. It was previously demonstrated that certain pathogenic forms of Aβ induce several pathologic responses in these cells, including fibril assembly at the cell surface, increased levels of Aβ precursor, degradation of HCSM cell α‐actin and cell death. The recently discovered novel rescue factor humanin (HN) was shown to protect neuronal cells in culture from various AD‐relevant insults including treatment with Aβ. In this report we investigated whether the HN peptide could rescue HCSM cells from Aβ‐induced toxicity. We found that treatment of HCSM cells with 10 μ m HN prevented pathogenic Aβ‐induced HCSM cell death using a fluorescent cell viability assay, and degradation of HCSM α‐actin was diminished shown by quantitative immunoblotting. However, Aβ deposition and fibril formation at the cell surface and increased levels of cell‐associated AβPP were not affected by treatment with HN as demonstrated by a thioflavin T fluorescence assay and immunochemical methods, respectively. These results suggest that the protective effects of HN occur downstream of these cell surface molecular events. This is the first demonstration of a rescue factor for HCSM cells from Aβ‐mediated cell death as well as being the first report to show that neuronal cells and HCSM cells may share a common downstream mechanism in the Aβ‐induced cell death pathway.
Absorption Spectra, Luminescence Properties, and Electrochemical Behavior of Cyclometalated Iridium(III) and Rhodium(III) Complexes with a Bis(pyridyl)triazole Ligand (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAbsorption Spectra, Luminescence Properties, and Electrochemical Behavior of Cyclometalated Iridium(III) and Rhodium(III) Complexes with a Bis(pyridyl)triazole LigandGiuseppe Calogero, Gaetano Giuffrida, Scolastica Serroni, Vittorio Ricevuto, and Sebastiano CampagnaCite this: Inorg. Chem. 1995, 34, 3, 541–545Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://pubs.acs.org/doi/10.1021/ic00107a004https://doi.org/10.1021/ic00107a004research-articleACS PublicationsRequest reuse permissionsArticle Views930Altmetric-Citations97LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Opioid genetics: the key to personalized pain control? (2012)

There are now several strong opioids available to choose from for the relief of moderate to severe pain. On a population level, there is no difference in terms of analgesic efficacy or adverse reactions between these drugs; however, on an individual level there is marked variation in response to a given opioid. The genetic influences to this variation are complex, and although current research has shown some promising results, these have not been replicated across larger studies and as such the ultimate aim of personalized prescribing remains elusive. If personalized prescribing could be achieved this would have a major impact at an individual level to facilitate safe, effective and rapid symptom control. This review presents some of the recent positive advances in opioid pharmacogenetic studies, focusing on associations between candidate genes and the three main elements of opioid response: analgesic, upper gastrointestinal and central adverse reactions.
Bioavailability of controlled release mesalazine (5-ASA) preparations. (1995)

Salazosulfapyridine was generated by Nanna Svartz in 1941. Mesalazine (5-ASA) was demonstrated in 1977 to be the therapeutically active moiety of salazosulfapyridine. Since then, a number of alternatives to salazosulfapyridine have appeared: olsalazine and salicylazobensoic acid, which are as salazosulfapyridine azo-preparations. Also, pure mesalazine drugs have been developed: Asacol, Claversal, and Pentasa. Despite the many similarities of these pure mesalazine controlled-release drugs, there are also significant differences. Some of these are due to different pharmaceutical designs of the delivery principle. Other important parameters are gastrointestinal pH variations in the healthy and diseased gut, and variations in ventricular emptying and in intestinal transit patterns.
Expression of cilium-associated genes defines novel molecular subtypes of idiopathic pulmonary fibrosis (2013)

Expression of cilium genes appears to identify two unique molecular phenotypes of IPF/UIP. The different molecular profiles may be relevant to therapeutic responsiveness in patients with IPF/UIP.
Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. (1983)

The functional and phenotypic characteristics of cells in human peripheral blood that mediate "natural killer" (NK) cytolysis have been examined with the use of multiparameter flow cytometry analysis and cell sorting. Essentially, all lymphocytes expressing NK and ADCC activity reacted with the anti-Leu-11a monoclonal antibody. The Leu-11a antigen was expressed on cytotoxic large granular lymphocytes (LGL), neutrophils, and basophils, but was not present on B cells, mitogen-activated T lymphoblasts, or Leu-1+ and Leu 4+ resting T cells. Anti-Leu-11a antibody selectively inhibited the binding of FITC heat-aggregated IgG complexes to granulocytes and LGL, and it may recognize a type of Fc receptor on these cells. Two-color FACS cell sorting indicated the existence of four lymphocyte subsets defined by the expression of Leu-11a and Leu-7 antigens. The Leu-11a+, -7- cells were highly active in 4-hr NK assays with the use of 51Cr-labeled K562 as the target. In contrast, the Leu-11a-, -7+ cells demonstrated weak activity and the Leu-11a-, -7- cells demonstrated no activity. The function of the Leu 11a+, -7+ cells varied considerably among several individuals examined. Multiparameter analysis with the use of two-color flow cytometry was used to determine the relationship between the expression of these NK-associated antigens and T and B cell-associated markers. These data indicate that considerable heterogeneity exists within human peripheral lymphocytes with regard to cell phenotype and function, but that several defined cellular subsets can be clearly revealed by using multiparameter FACS analysis and sorting.
Ultrathin Two-Dimensional Atomic Crystals as Stable Interfacial Layer for Improvement of Lithium Metal Anode (2014)

Stable cycling of lithium metal anode is challenging due to the dendritic lithium formation and high chemical reactivity of lithium with electrolyte and nearly all the materials. Here, we demonstrate a promising novel electrode design by growing two-dimensional (2D) atomic crystal layers including hexagonal boron nitride (h-BN) and graphene directly on Cu metal current collectors. Lithium ions were able to penetrate through the point and line defects of the 2D layers during the electrochemical deposition, leading to sandwiched lithium metal between ultrathin 2D layers and Cu. The 2D layers afford an excellent interfacial protection of Li metal due to their remarkable chemical stability as well as mechanical strength and flexibility, resulting from the strong intralayer bonds and ultrathin thickness. Smooth Li metal deposition without dendritic and mossy Li formation was realized. We showed stable cycling over 50 cycles with Coulombic efficiency ∼97% in organic carbonate electrolyte with current density and areal capacity up to the practical value of 2.0 mA/cm(2)and 5.0 mAh/cm(2), respectively, which is a significant improvement over the unprotected electrodes in the same electrolyte.
Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus. (1993)

This study analyzes the hierarchy of signals that spatially restrict expression of Xenopus Xwnt-8 to mesodermal cells outside of the Spemann organizer field and examines the potential role that endogenous Xwnt-8 may play in dorsoventral patterning of the embryonic mesoderm. The effects of ectopic introduction of a Nieuwkoop center-like activity or of ectopic expression of goosecoid, on the distribution of endogenous Xwnt-8 transcripts were analyzed. The results of these studies are consistent with the hypothesis that maternally derived signals from the Nieuwkoop center function to positively regulate expression of the homeo box gene goosecoid in Spemann organizer cells, leading to a subsequent repression of Xwnt-8 expression in these cells. This exclusion of Xwnt-8 from cells of the organizer field may be important for normal dorsal development, in that ectopic expression of Xwnt-8 in organizer cells after the midblastula stage, by injection of plasmid DNA, ventralizes the fate of these cells. This is distinct from the previously observed dorsalizing effect of Xwnt-8 when expressed prior to the midblastula stage by injection of RNA. The effects of plasmid-derived Xwnt-8 on isolated blastula animal cap ectoderm were also analyzed. Expression of Xwnt-8 in animal pole ectoderm after the midblastula stage ventralizes the response of dorsal animal pole cells to activin and allows naive ectodermal cells to differentiate as ventral mesoderm in the absence of added growth factors. Collectively, these data are consistent with the hypothesis that Xwnt-8 plays a role in the mesodermal differentiation of ventral marginal zone cells during normal development. Furthermore, endogenous Xwnt-8 may ventralize the response of lateral mesodermal cells to dorsalizing signals from the organizer, thus contributing to the graded nature of the final body pattern.
Transient replication of human papillomavirus DNAs (1992)

Information on papillomavirus DNA replication has primarily derived from studies with bovine papillomavirus type 1 (BPV-1). Our knowledge of DNA replication of the human papillomaviruses (HPVs) is quite limited, in part because of the lack of a cell culture system capable of supporting the stable replication of HPV DNA. This study demonstrates that the full-length genomic DNAs of HPV types 11 and 18 (HPV-11 and HPV-18), but not HPV-16, are able to replicate transiently after transfection into several different human squamous cell carcinoma cell lines. This system was used to identify the viral cis and trans elements required for DNA replication. The viral origins of replication were localized to a region of the viral long control region. Like BPV-1, E1 and E2 were the only viral factors required in trans for the replication of plasmids containing the origin. Cotransfection of a plasmid expressing the E1 open reading frame (ORF) from HPV-11 with a plasmid that expresses the E2 ORF from HPV-6, HPV-11, HPV-16, or HPV-18 supported the replication of plasmid DNAs containing the origin regions of HPV-11, HPV-16, or HPV-18, indicating that there are functions shared among the corresponding E1 and E2 proteins and origins of these viruses. Although HPV-16 genomic DNA did not replicate by itself under experimental conditions that supported the replication of HPV-11 and HPV-18 genomic DNAs, expression of the HPV-16 early region functions from a strong heterologous promoter supported the replication of a cotransfected plasmid containing the HPV-16 origin of replication. This finding suggests that the inability of the HPV-16 genomic DNA to replicate transiently in the cell lines tested was most likely due to insufficient expression of the viral E1 and/or E2 genes required for DNA replication.
RANTES chemokine expression in diseased and normal human tissues (1996)

RANTES is a member of a large family of cytokines, called chemokines, which are thought to play a regulatory role in inflammatory processes. We have made recombinant human RANTES protein which was used to generate a panel of anti-RANTES monoclonal antibodies. Following characterization, select anti-RANTES monoclonal antibodies were used for immunohistologic staining of a large panel of normal, diseased and fetal tissue sections. Diseased tissues included eleven lymphomas and eight renal tumors. Most tissues were also tested in parallel for RANTES mRNA by in situ hybridization using RANTES mRNA specific oligomeric probes. As expected, most normal adult tissues contain few, if any, RANTES positive cells. In contrast, RANTES expression dramatically increases in inflammatory sites. In addition, megakaryocytes, some tumours, and select fetal tissues express high levels of RANTES message and protein. These results indicate a wider expression of RANTES than previously appreciated and suggest multiple physiologic roles for this soluble factor.