gebhart/patrepeval huggingface dataset:
Elevated serum IL-10 levels in diffuse large B-cell lymphoma: a mechanism of aberrant JAK2 activation (2012)

Cytokines are deregulated in cancers and can contribute to tumor growth. In patients with diffuse large-cell lymphoma (DLBCL), we observed higher levels of JAK/STAT pathway-related serum cytokines (ie, IL-6, IL-10, epidermal growth factor, and IL-2) compared with controls. Of these, only IL-10 activated the JAK2 pathway in lymphoma cells in vitro. Patients with high serum IL-10 had shorter event-free survival (EFS) than patients with low levels (P > .01) and high IL-10 was correlated with high lactase dehydrogenase (P = .0085) and higher International Prognostic Index scores (P = .01). To explore the mechanism by which IL-10 may contribute to an inferior EFS, we investigated the effect of IL-10 on the JAK2 pathway and found that the IL-10/IL-10 receptor complex up-regulated JAK2 signaling. Neutralizing Ab to IL-10 inhibited constitutive and IL-10-induced JAK2/STAT3 phosphorylation. JAK2 inhibition dephosphorylated JAK2 and STAT3 and caused an inhibitory effect on phospho-JAK2-positive DLBCL cells; there was a minimal effect on phospho-JAK2-negative cells. Apoptosis induced by JAK2 inhibition was dependent on inhibition of autocrine IL-10 and c-myc expression and independent of Bcl-2 family expression. These results provide the rationale for testing JAK2 inhibitors in DLBCL patients, and indicate that serum IL-10 may be a biomarker to identify patients more likely to respond to JAK2-targeted therapy.
Identification of RNF213 as a Susceptibility Gene for Moyamoya Disease and Its Possible Role in Vascular Development (2011)

Background: Moyamoya disease is an idiopathic vascular disorder of intracranial arteries. Its susceptibility locus has been mapped to 17q25.3 in Japanese families, but the susceptibility gene is unknown.
Lipopolysaccharide Activates Distinct Signaling Pathways in Intestinal Epithelial Cell Lines Expressing Toll-Like Receptors (2000)

LPS elicits several immediate proinflammatoy responses in peripheral blood leukocytes via a recently described pathway including CD14, Toll-like receptors (TLR), serine-threonine kinases, and NF-kappaB transcription factor. However, the functional responses of intestinal epithelial cells (IEC) to stimulation with LPS are unknown. Expression of mRNA and protein for CD14 and TLRs were assessed by RT-PCR, immunoblotting, and immunohistochemistry in mouse and human IEC lines. LPS-induced activation of signaling pathways (p42/p44 mitogen-activated protein kinase (MAPK), c-Jun NH2-terminal kinase (JNK), p38, p65, NF-kappaB) were assessed by immunoblotting and gel shifts. CD14 mRNA and protein expression were not detectable in IEC. However, human TLR2, TLR3, and TLR4 mRNA were present in IEC. TLR4 protein was expressed in all cell lines; however, TLR2 protein was absent in HT29 cells. Immunofluorescent staining of T84 cells demonstrated the cell-surface presence of the TLRs. LPS-stimulation of IEC resulted in activation (>1.5-fold) of the three members of the MAPK family. In contrast, LPS did not significantly induce activation of JNK and p38 in CMT93 cells, p38 in T84 cells and MAPK and JNK in HT29 cells. Downstream, LPS activated NF-kappaB in IEC in a time-, dose-, and serum-dependent manner. IEC express TLRs that appear to mediate LPS stimulation of specific intracellular signal transduction pathways in IEC. Thus, IEC may play a frontline role in monitoring lumenal bacteria.
Pyridine Rings as Protected 2° Amines:  Facile Hydrogenation of Heterocyclic Aromatic Polymers (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTPyridine Rings as Protected 2° Amines: Facile Hydrogenation of Heterocyclic Aromatic PolymersYoko Aoyama and Bruce M. NovakView Author Information Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts 01003, and Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695 Cite this: Macromolecules 2001, 34, 20, 6842–6844Publication Date (Web):August 18, 2001Publication History Received11 June 2001Published online18 August 2001Published inissue 1 September 2001https://pubs.acs.org/doi/10.1021/ma011013fhttps://doi.org/10.1021/ma011013frapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views405Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Amines,Catalysts,Hydrogenation,Polymers,Pyridines Get e-Alerts
Comparative binding and preclinical localization and therapy studies with radiolabeled human chimeric and murine 17-1A monoclonal antibodies. (1990)

Murine MoAb 17-1A is an IgG2a antibody reactive with a gastrointestinal cancer-associated cell surface antigen. Human-mouse chimeric 17-1A MoAbs were constructed in which the murine variable region of 17-1A was joined with human IgG1, IgG2, IgG3, and IgG4 constant regions. Human-mouse IgG1, IgG2, and IgG4 chimeric antibodies were compared with the parental murine antibody and its F(ab')2 fragments for their ability to bind to colon carcinoma cells in vitro, for their blood clearance in normal nude mice, and for their localization and tumor growth inhibition of colon carcinoma xenografts in nude mice. Indirect immunofluorescence experiments with fluorescein-conjugated goat anti-mouse or goat anti-human antibody verified that the substitution of human constant regions in the chimeric MoAbs did not significantly alter the ability of the murine variable region to bind to colon adenocarcinoma cell lines (LS174T, SW948, and C0112). The immunoreactivities of 125I-labeled murine and chimeric 17-1A MoAbs measured in a live cell-binding assay with LS174T, SW948, and C0112 cells revealed that chimeric IgG1, IgG4, and 17-1A F(ab')2 were comparable to murine 17-1A while chimeric IgG2 showed lower binding. The blood half-lives of 125I-labeled murine 17-1A, its F(ab')2 fragments, and chimeric IgG1, IgG2, and IgG4 in normal nude mice determined by serial eye bleeding were 7.5, 0.5, 5.2, 6.9, and 1.9 days, respectively. In biodistribution studies at 4 days after injection of 125I-labeled MoAbs in nude mice bearing LS174T tumors, chimeric IgG1 had the highest tumor concentration of 20.5% injected dose/g with a tumor/blood ratio of 3.2. 131I-labeled murine 17-1A administered in a single injection of 300 microCi or 3 injections of about 300 microCi each to nude mice bearing established LS174T tumors inhibited tumor growth, whereas a comparable amount of unlabeled murine 17-1A did not inhibit tumor growth. 131I-labeled chimeric IgG1 MoAb showed a similar level of tumor growth inhibition. The results of the present study indicate that 17-1A chimeric IgG1 antibody may be the best choice for clinical radioimmunodetection and radioimmunotherapy studies.
Disturbances in carnitine metabolism (1986)

Conference Article| August 01 1986 Disturbances in carnitine metabolism PEGGY R. BORUM PEGGY R. BORUM 1409 Food Science Building, University of Florida, Gainesville, FL 32611, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1986) 14 (4): 681–683. https://doi.org/10.1042/bst0140681 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation PEGGY R. BORUM; Disturbances in carnitine metabolism. Biochem Soc Trans 1 August 1986; 14 (4): 681–683. doi: https://doi.org/10.1042/bst0140681 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1986 Biochemical Society1986 Article PDF first page preview Close Modal You do not currently have access to this content.
Pyrimidine derivatives. VI. Synthesis of 2-(1-piperazinyl)-5,6-polymethylenepyrimidine derivatives and determination of their hypoglycemic activity. (1983)

As part of our studies on pyrimidine derivatives, 27 4-alkyl-2-(1-piperazinyl)-5, 6-polymethylenepyrimidines and structurally related derivatives were synthesized, and their hypoglycemic activities were examined in mice treated with 2-deoxy-D-glucose. Most of the derivatives showed higher activity at a dose of 30 mg/kg p.o. than tolbutamide at a dose of 100 mg/kg p.o. The structure-activity relationships are discussed.
Biomimetic emulsions reveal the effect of mechanical forces on cell–cell adhesion (2012)

Cell-cell contacts in tissues are continuously subject to mechanical forces due to homeostatic pressure and active cytoskeleton dynamics. In the process of cellular adhesion, the molecular pathways are well characterized but the role of mechanics is less well understood. To isolate the role of pressure we present a dense packing of functionalized emulsion droplets in which surface interactions are tuned to mimic those of real cells. By visualizing the microstructure in 3D we find that a threshold compression force is necessary to overcome electrostatic repulsion and surface elasticity and establish protein-mediated adhesion. Varying the droplet interaction potential maps out a phase diagram for adhesion as a function of force and salt concentration. Remarkably, fitting the data with our theoretical model predicts binder concentrations in the adhesion areas that are similar to those found in real cells. Moreover, we quantify the dependence of the area of adhesion on the applied force and thus reveal adhesion strengthening with increasing external pressure even in the absence of active cellular processes. This biomimetic approach reveals a physical origin of pressure-sensitive adhesion and its strength across cell-cell junctions.
Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers. (1983)

Usual human livers contain two major aldehyde dehydrogenase isozymes, cytosolic ALDH1 component and mitochondrial ALDH2 component, while human livers with "atypical" phenotype have only ALDH1 isozyme and are missing ALDH2 isozyme. Approximately 50% of orientals are atypical in respect to ALDH isozymes. We previously demonstrated an existence of enzymatically inactive but immunologically cross-reactive material (CRM) in atypical oriental livers. ALDH1 and ALDH2 isozymes were purified to homogeneity from usual livers, and ALDH1 and CRM were purified from atypical oriental livers. Amino acid compositions of ALDH1 and ALDH2 were similar to, but not identical with, each other. Amino acid compositions of ALDH2 and CRM were identical within analytical errors. Subunit molecular size of ALDH1 estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 56,200 daltons, and that of ALDH2 was 52,600 daltons. The two isozymes did not contain a common subunit. Subunit molecular weight of CRM was identical with that of ALDH2. Double immunodiffusion precipitation revealed that ALDH1 and ALDH2 were immunologically analogous but not identical, and that CRM and ALDH2 were immunologically indistinguishable. These results support the genetic model that CRM is an abnormal defective protein resulting from a mutation of the ALDH2 locus.
Test Review (1997)

The CSBS is intended to generate profiles of strengths and weaknesses for a child across communicative, social-affective, and symbolic domains of development for use in diagnostic assessment and intervention planning. Because of the well documented variability in normal development of language, the sensitivity to different rates of growth in children is an important part of the CSBS. In order for abnormal variability to be detected, normal variability must be sufficiently handled by the conversion of raw scores to standard scores. Goodwyn (1995) examined the variability of CSBS standard scores over time in a small sample of normally developing infants using generalizability theory (Cronbach, Gleser, Nanda, &amp; Rajaratnam, 1972). Generally, her findings indicated sensitivity of the instrument to varying rates of development across children, sensitivity to changes in children's abilities over time, and proper functioning of the normed scores. The relatively small sample size (N = 282) used in the development of the instrument's norms is an aspect of the CSBS that could be cause for concern. Yet, Goodwyn (1995) found no evidence for improper functioning of the norms. Some limitations regarding the administration and scoring of the instrument are worth noting. We found a significant disparity in the estimated and actual time required to score the videotaped samples. Even after training and experience with the instrument, we found that scoring took a minimum of one hour and forty-five minutes. Although we have not reveiwed the Scoring Tutorial tapes, it is likely that they will be helpful due to the complexity of the scoring. Applied Symbolix is scheduled to release the CSBS Quick Score in the near future. The Quick Score is designed to reduce the complexity of recording and scoring the instrument. A reordering of the testing procedure during the Language Comprehension portion may be advisable. As the objective is to assess the child's linguistic comprehension, we suggest measuring this while the child is initially focused on the doll prior to presenting the toy sets in the Symbolic Play Probes section of the testing procedure. We have found during testing, that once children have been presented with the toy sets in the Symbolic Play Probes section, they are reluctant to go back to focus only on the doll to identify body parts in the Language Comprehension Probes section. Reordering the testing procedure in this way may cause the norms for the symbolic cluster scale and communication composite score to change slightly. Increased awareness of the need for early detection and intervention of disorders in children was underscored by the inclusion of children under the age of five in the Individuals with Disabilities Education Act. The CSBS has potential to be a useful tool for early identification and intervention planning of language and communication disorders for this age group. The standardized behavior sampling used in the CSBS is unique; with further development, the CSBS may become the instrument of choice for assessing communication and symbolic abilities in very young children.
Joint motion in an open MR unit using MR tracking (1999)

A system for active scan plane guidance during kinematic magnetic resonance (MR) examination of joint motion was developed utilizing an external tracking coil and MR tracking software. In a phantom study and during upright, weight-bearing, physiologic knee flexion, the external tracking coil maintained the scan plane through desired structures. Thus, MR tracking provides a robust method to guide the scan plane during MR imaging of active joint motion.
Thrombin Generation Assay and Viscoelastic Coagulation Monitors Demonstrate Differences in the Mode of Thrombin Inhibition Between Unfractionated Heparin and Bivalirudin (2007)

Thrombus formation kinetics differs between heparin and bivalirudin despite similar prolongation of clotting test values.
Prospective patient stratification into robust cancer‐cell intrinsic subtypes from colorectal cancer biopsies (2018)

Colorectal cancer (CRC) biopsies underpin accurate diagnosis, but are also relevant for patient stratification in molecularly-guided clinical trials. The consensus molecular subtypes (CMSs) and colorectal cancer intrinsic subtypes (CRISs) transcriptional signatures have potential clinical utility for improving prognostic/predictive patient assignment. However, their ability to provide robust classification, particularly in pretreatment biopsies from multiple regions or at different time points, remains untested. In this study, we undertook a comprehensive assessment of the robustness of CRC transcriptional signatures, including CRIS and CMS, using a range of tumour sampling methodologies currently employed in clinical and translational research. These include analyses using (i) laser-capture microdissected CRC tissue, (ii) eight publically available rectal cancer biopsy data sets (n = 543), (iii) serial biopsies (from AXEBeam trial, NCT00828672; n = 10), (iv) multi-regional biopsies from colon tumours (n = 29 biopsies, n = 7 tumours), and (v) pretreatment biopsies from the phase II rectal cancer trial COPERNCIUS (NCT01263171; n = 44). Compared to previous results obtained using CRC resection material, we demonstrate that CMS classification in biopsy tissue is significantly less capable of reliably classifying patient subtype (43% unknown in biopsy versus 13% unknown in resections, p = 0.0001). In contrast, there was no significant difference in classification rate between biopsies and resections when using the CRIS classifier. Additionally, we demonstrated that CRIS provides significantly better spatially- and temporally- robust classification of molecular subtypes in CRC primary tumour tissue compared to CMS (p = 0.003 and p = 0.02, respectively). These findings have potential to inform ongoing biopsy-based patient stratification in CRC, enabling robust and stable assignment of patients into clinically-informative arms of prospective multi-arm, multi-stage clinical trials. © 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
Quantum optical properties of a dipole emitter coupled to an ɛ-near-zero nanoscale waveguide (2013)

We study quantum optical properties of a dipole emitter coupled to a rectangular nanoscale waveguide with dielectric core and silver cladding. We investigate enhanced spontaneous emission and the photonic Lamb shift for emitters whose resonant frequencies are near the waveguide frequency cutoff where the waveguide behaves as an ɛ-near-zero metamaterial. Via a dyadic Green's function-based field quantization scheme, we calculate the photonic Lamb shift as well as the spontaneous emission enhancement and spectrum. Using realistic parameters for typical quantum emitters, we suggest experimentally realizable schemes to observe relatively large photonic Lamb shifts in waveguides.
Instrumented One and Two Level Posterolateral Fusions With Recombinant Human Bone Morphogenetic Protein-2 and Allograft (2009)

rhBMP-2 (Infuse) at a dose of 12 mg/1.5 mg/mL combined with freeze-dried corticancellous allograft and local autogenous spinous process and lamina bone resulted in new bone formation (fusion) as determined by CT scan. In this preliminary study, fusion rates were 97.2% with consistent posterolateral bone formation. Outcomes and complications are consistent with other studies of this type.
Neurotoxic effects of low doses of glutamate on purified rat retinal ganglion cells. (1998)

Low doses of glutamate can activate AMPA-KA receptors in RGCs, which causes increases in intracellular calcium and decreases in cell survival. This is the first report to show the functional role of calcium-permeable AMPA-KA receptors in cultured RGCs.
Volatile Compounds Formed from Thermal Degradation of Glucosamine in a Dry System (1998)

Glucosamine was pyrolyzed at 200 °C for 30 min under dry conditions. A roasty aroma was obtained. Volatile compounds generated included pyrazines, pyridines, pyrroles, and furans. The most interesting compounds were pyrazines with one or two furyl substituents. They are 2-(2-furyl)pyrazines, 2-(2-furyl)methylpyrazines, 2-(2-furyl)dimethylpyrazines, 2-(2-furyl)acetylpyrazines, and di-2-furylpyrazines. Some of these furyl pyrazines are being reported for the first time. The mechanism of the furan ring formation in polycyclic pyrazine compounds was studied. The furan ring was suggested as a result of the dehydration reaction from the intermediate polyhydroxypyrazines, which were dimerization products of glucosamine. Model reactions were designed to test the proposed mechanism. When fructose and ammonium acetate were heated under the same conditions as the glucosamine pyrolysis, those furyl pyrazines were also generated. Keywords: Glucosamine; 2-amino-2-deoxyglucose; Maillard reaction; volatile compound; pyrazines; furylpyrazines
Current Report: Office of Health Information, Health Promotion and Physical Fitness and Sports Medicine (1980)

(1980). Current Report: Office of Health Information, Health Promotion and Physical Fitness and Sports Medicine. Health Education: Vol. 11, No. 2, pp. 28-28.