gebhart/patrepeval huggingface dataset:
Glance behaviours when using an in-vehicle smart driving aid: A real-world, on-road driving study (2013)

In-vehicle information systems (IVIS) are commonplace in modern vehicles, from the initial satellite navigation and in-car infotainment systems, to the more recent driving related Smartphone applications. Investigating how drivers interact with such systems when driving is key to understanding what factors need to be considered in order to minimise distraction and workload issues while maintaining the benefits they provide. This study investigates the glance behaviours of drivers, assessed from video data, when using a smart driving Smartphone application (providing both eco-driving and safety feedback in real-time) in an on-road study over an extended period of time. Findings presented in this paper show that using the in-vehicle smart driving aid during real-world driving resulted in the drivers spending an average of 4.3% of their time looking at the system, at an average of 0.43 s per glance, with no glances of greater than 2 s, and accounting for 11.3% of the total glances made. This allocation of visual resource could be considered to be taken from ‘spare’ glances, defined by this study as to the road, but off-centre. Importantly glances to the mirrors, driving equipment and to the centre of the road did not reduce with the introduction of the IVIS in comparison to a control condition. In conclusion an ergonomically designed in-vehicle smart driving system providing feedback to the driver via an integrated and adaptive interface does not lead to visual distraction, with the task being integrated into normal driving.
Mitomycin C: a prototype bioreductive agent. (1994)

Hypoxic cells of solid tumors represent a therapeutically resistant population that limits the curability of many solid tumors by x-irradiation and by most chemotherapeutic agents. The oxygen deficit, however, creates an environment conducive to reductive processes that results in a major exploitable difference between normal and neoplastic tissues. Mitomycin C (MC) can be reductively activated by a number of oxidoreductases, in a process required for the production of its therapeutic effects. This enzymatic reduction results in preferential activation of MC under hypoxia and, in most instances, the production of greater toxicity to oxygen-deficient cells than to their oxygenated counterparts. DNA appears to be the most important target of the reactive species generated from MC, with both mono- and bis-adducts of DNA being formed in drug-treated cells. The demonstration that MC, used to kill the hypoxic fraction, in combination with x-irradiation, to eradicate the oxygenated portion of the tumor, produced enhanced cytodestructive effects on solid tumors of animals has led to the clinical evaluation of the mitomycin antibiotics in combination with x-rays in patients with cancers of the head and neck. The findings from these clinical trials have demonstrated the utility of directing a concerted therapeutic attack on the hypoxic fraction of solid tumors as an approach toward enhancing the curability of localized neoplasms by x-irradiation.
The VP35 and VP40 proteins of filoviruses (1993)

The fragments of genomic RNA sequences of Marburg (MBG) and Ebola (EBO) viruses are reported. These fragments were found to encode the VP35 and VP40 proteins. The canonic sequences were revealed before and after each open reading frame. It is suggested that these sequences are mRNA extremities and at the same time the regulatory elements for mRNA transcription. Homology between the MBG and EBO proteins was discovered.
CMV Antigen-Specific CD4<SUP>+</SUP> and CD8<SUP>+</SUP> T Cell IFNγ Expression and Proliferation Responses in Healthy CMV-Seropositive Individuals (2004)

CMV-specific CD4+ and CD8+ T cell IFNγ expression and proliferation were measured in healthy volunteers by flow cytometry after CMV lysate or CMV pp65 or IE peptide pool stimulation. Cutoff values were set to maximize specificity (i.e., no false positive CMV-seronegatives). Sensitivity (defined as a positive response in CMV-seropositives to at least one of the 3 antigen preparations used) was 100% for CMV-specific CD4+ and CD8+ T cell IFNg expression and CD4+ T cell proliferation and 95.4% for CMV-specific CD8+ T cell proliferation. All 22 CMV-seropositive individuals had positive responses by at least three of these four measurements. These findings support the concept that a multiplicity of antigen-specific functional immune responses and persistence of robust virus-specific CD4+ T cells are important components of protective immunity in this chronic viral infection.
Waxy Endosperm Mutants of Bread Wheat(Triticum aestivum L.) and Their Starch Properties. (1997)

Two waxy bread wheat lines, KI07Wx1 and KI07Wx2, have been developed by treating cv. Kanto 107 seeds with ethyl methanesulphonate (EMS). About 2, 000 seeds of the cultivar were soaked for 4 hours in 0.5% EMS dissolved in 7% ethanol, and then 2, 000 seeds were sown in a green-house and 10, 634 M2 seeds were obtained from 1, 872 M1 plants. Cross sections of the distal endosperms of 4, 000 M2 seeds were stained with an iodine-potassium iodide solution, and two seeds stained red-brown. Since the M3 and M4 seeds derived from the remaining proximal halves of these two seeds showed the characteristic feature of waxy endo-sperm, the waxy endosperm character was considered to be genetically fixed in these two mutant lines, which were designated as KI07Wx1 and KI07Wx2. The amylose content of endosperm starch from both mutant lines was 0.9%, and no waxy proteins were detected in the starch of either line. The agronomic characters of KI07Wx1 and KI07Wx2 were very similar to those of cv. Kanto 107 grown under field conditions, except for the heading dates of the mutant lines and the 1, 000-grain weight of KI07Wx2. These results indicate that the mutagen treatment of seeds is effective in inducing of waxy, i.e. amylose-free, mutants when a bread wheat cultivar has a single waxy protein in their endosperm starch.
Orthogonal Combinatorial Chemical Libraries (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrthogonal Combinatorial Chemical LibrariesBenoit Deprez, Xavier Williard, Line Bourel, Herve Coste, Francois Hyafil, and Andre TartarCite this: J. Am. Chem. Soc. 1995, 117, 19, 5405–5406Publication Date (Print):May 1, 1995Publication History Published online1 May 2002Published inissue 1 May 1995https://pubs.acs.org/doi/10.1021/ja00124a042https://doi.org/10.1021/ja00124a042research-articleACS PublicationsRequest reuse permissionsArticle Views260Altmetric-Citations79LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Plasmons and high-temperature superconductivity in alloys of copper oxides (1987)

The two-dimensional character of the electronic structure of M-N-Cu-O alloys, with M=La,Y and N=Ba,Sm,..., is shown to favor the formation of ``acoustic'' plasmons at energies above the acoustic phonons. Providing that the Fermi energy intersects a small pocket of electrons (or holes) in addition to the expected occupation of a primary electron (or hole) band, the plasma oscillations of the secondary charge carriers may provide a mechanism for room-temperature superconductivty.
A Dimer as a Building Block in Assembling RNA (2000)

Six RNA (pRNA) molecules form a hexamer, via hand-in-hand interaction, to gear bacterial virus phi29 DNA translocation machinery. Here we report the pathway and the conditions for the hexamer formation. Stable pRNA dimers and trimers were assembled in solution, isolated from native gels, and separated by sedimentation, providing a model system for the study of RNA dimers and trimers in a protein-free environment. Cryo-atomic force microscopy revealed that monomers displayed a ✓ outline, dimers exhibited an elongated shape, and trimers formed a triangle. Dimerization of pRNA was promoted by a variety of cations including spermidine, whereas procapsid binding and DNA packaging required specific divalent cations, including Mg<sup>2+</sup>, Ca<sup>2+</sup>, and Mn<sup>2+</sup>. Both the tandem and fused pRNA dimers with complementary loops designed to form even-numbered rings were active in DNA packaging, whereas those without complementary loops were inactive. We conclude that dimers are the building blocks of the hexamer, and the pathway of building a hexamer is: dimer → tetramer → hexamer. The Hill coefficient of 2.5 suggests that there are three binding sites with cooperative binding on the surface of the procapsid. The two interacting loops played a key role in recruiting the incoming dimer, whereas the procapsid served as the foundation for hexamer assembly.
Effect of surface ligands on the performance of organic light-emitting diodes containing quantum dots (2014)

Quantum dots (QDs) have numerous applications in optoelectronics due to their unique optical properties. Novel hybrid organic light-emitting diodes (OLEDs) containing QDs as an active emissive layer are being extensively developed. The performance of QD–OLED depends on the charge transport properties of the active layer and the degree of localization of electrons and holes in QDs. Therefore, the type and the density of the ligands on the QD surface are very important. We have fabricated OLEDs with a CdSe/ZnS QD active layer. These OLEDs contain hole and electron injection layers consisting of poly(9-vinyl carbazole) and ZnO nanoparticles, respectively. The energy levels of these materials ensure efficient injection of charge carriers into the QD emissive layer. In order to enhance the charge transfer to the active QD layer and thereby increase the OLED efficiency, the QD surface ligands (tri-n-octyl phosphine oxide, TOPO) were replaced with a series of aromatic amines and thiols. The substituents were expected to enhance the charge carrier mobility in the QD layer. Surprisingly, the devices based on the original TOPO-coated QDs were found to have the best performance, with a maximum brightness of 2400 Cd/m<sup>2</sup> at 10 V. We assume that this was due to a decrease in the charge localization within QDs when aromatic ligands are used. We conclude that the surface ligands considerably affect the performance of QD–OLEDs, efficient charge localization in QD cores being more important for good performance than a high charge transfer rate.
Syndecan‐3 and perlecan are differentially expressed by progenitors and mature oligodendrocytes and accumulate in the extracellular matrix (2002)

Abstract Oligodendrocyte progenitors originate in the subventricular zone, proliferate, migrate to their final destinations, differentiate, and interact with axons to produce multilamellar myelin sheaths. These processes are regulated by a variety of environmental signals, including growth factors, the extracellular matrix, and adhesion molecules. Heparan sulfate proteoglycans are premier candidates as participants in this regulation by virtue of their structural diversity and their capacity to function as coreceptors for both growth factors and extracellular matrix molecules. Consistently with this, we have previously shown that oligodendrocyte progenitors are unable to proliferate in response to fibroblast growth factor‐2 (FGF‐2) in the absence of sulfated heparan sulfate proteoglycan. Here we show that members of three families of heparan sulfate proteoglycans, syndecan, perlecan, and glypican, are developmentally and posttranscriptionally regulated during oligodendrocyte‐lineage progression: Syndecan‐3 is synthesized by oligodendrocyte progenitors (but not terminally differentiated oligodendrocytes) and is up‐regulated by FGF‐2; perlecan synthesis increases as oligodendrocytes undergo terminal differentiation; glypican‐1 is expressed by both progenitors and differentiated oligodendrocytes. Astrocytes express glypican‐1 and perlecan but not syndecan‐3. All three of these heparan sulfate proteoglycans are shed from the cell surface and bind to specific substrates. The developmentally regulated expression of these heparan sulfate proteoglycans is indicative of their participation in events involving growth factor receptors and the extracellular matrix that may regulate oligodendrocyte progenitor proliferation, migration, and adhesion phenomena. © 2002 Wiley‐Liss, Inc.
Variable Region Sequences of Five Human Immunoglobulin Heavy Chains of the V H III Subgroup: Definitive Identification of Four Heavy Chain Hypervariable Regions (1974)

The variable regions of five human immunoglobulin heavy chains of the V(H)III subgroup have been totally sequenced. Three of the heavy chains belonged to the IgG class and two to the IgA class. Examination of these sequences, and comparison with additional published heavy chain sequences, showed that a total of four hypervariable regions is characteristic of human heavy chain variable regions. The relatively conserved character of large segments of the heavy chain variable region was very evident in these studies. The conserved segments, which are those sections located outside the hypervariable regions, comprise approximately 65% of the total heavy chain variable region. The following general structural pattern for antibody molecules emerges from this and related studies: an overall combining region superstructure is provided by the more conserved segments while the refinements of the active site specificity are a function of hypervariable regions.
The Subunits of Purified Rabbit Antibody* (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Subunits of Purified Rabbit Antibody*Sayaka Utsumi and Fred KarushCite this: Biochemistry 1964, 3, 9, 1329–1338Publication Date (Print):September 1, 1964Publication History Published online1 May 2002Published inissue 1 September 1964https://pubs.acs.org/doi/10.1021/bi00897a024https://doi.org/10.1021/bi00897a024research-articleACS PublicationsRequest reuse permissionsArticle Views168Altmetric-Citations102LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Adenosine Deaminase Inhibitors. Synthesis and Biological Evaluation of Putative Metabolites of (+)-erythro-9-(2S-Hydroxy-3R-nonyl)adenine (1994)

The synthesis and biological evaluation of three chain-hydroxylated (+)-erythro-9-(2S-hydroxy-3R-nonyl)adenine [(+)-EHNA] derivatives are reported. Hydroxy groups at positions 9', 8', and 8',9' (12, 25, and 16) were introduced by either epoxidation or hydroboration of a terminal olefinic intermediate. Affinities for calf intestinal adenosine deaminase (ADA) were determined from the steady-state inhibition of adenosine deamination. Ki values of 0.82, 3.8, 6.4, and 15.8 nM were estimated for (+)-EHNA, 9'-hydroxy-(+)-EHNA (12), 8'-hydroxy-(+)-EHNA (25), and 8',9'-dihydroxy-(+)-EHNA (16), respectively, by assuming a single class of binding sites. However, the data for all inhibitors conformed more closely to the kinetics of a heterogeneous system with different affinities for two or more binding sites. The fairly high potencies of 12 and 25 suggest that other substitutions at the terminal position of the nonyl chain could yield useful ADA inhibitors.
Adriamycin analogs. Preparation and biological evaluation of some N-perfluoroacyl analogs of daunorubicin, adriamycin, and N-(trifluoroacetyl)adriamycin 14-valerate and their 9,10-anhydro derivatives (1982)

The experimental and clinical antitumor activity, as well as the low toxicity, of N-(trifluoroacetyl)adriamycin 14-valerate (AD 32), a non-DNA binding anthracycline analogue, has led us to prepare and evaluate several N-perfluoroacyl analogues of daunorubicin, adriamycin, and N-(trifluoroacetyl)adriamycin 14-valerate. Target compounds were prepared by reaction of the appropriate perfluoroacyl anhydride with daunorubicin in chloroform-ether, with adriamycin in cold pyridine, and with adriamycin 14-valerate in ethyl acetate. In connection with this work, it was found that reaction of perfluoroacyl anhydrides with N-acylated or N-unsubstituted anthracyclines in pyridine at room temperature afforded with ease and in good yield the corresponding 9,10-anhydro-N-acylated derivatives. A number of products showed good to highly significant antitumor activity in vivo against the murine P388 leukemia system. However, the lack of in vivo antitumor activity of the pentafluoropropionyl and heptafluorobutyryl analogues of N-(trifluoroacetyl)adriamycin 14-valerate is noteworthy. The results continue to show that non-DNA binding anthracycline analogues can exhibit in vivo antitumor activity. Loss of the anthracycline 9-carbinol function by dehydration leads to reduction of biological activity as compared to the parent compound.
Human herpesvirus infections: Pathogenesis, diagnosis, and treatment (1986)

This book contains 24 selections. Some of the titles are: Molecular Biology of Latent HSV-1; Molecular Genetics of Antiviral Chemotherapy of Herpes Viruses; Molecular Basis of Foscarnet Action; Use of Vaccinia Virus as a Vector for Expression of Herpesvirus Genes; and Diagnosis of Herpesvirus with Monoclonal Antibodies.
Cationic Polystyrene Nanosphere Toxicity Depends on Cell-Specific Endocytic and Mitochondrial Injury Pathways (2007)

The exponential increase in the number of new nanomaterials that are being produced increases the likelihood of adverse biological effects in humans and the environment. In this study we compared the effects of cationic nanoparticles in five different cell lines that represent portal-of-entry or systemic cellular targets for engineered nanoparticles. Although 60 nm NH(2)-labeled polystyrene (PS) nanospheres were highly toxic in macrophage (RAW 264.7) and epithelial (BEAS-2B) cells, human microvascular endothelial (HMEC), hepatoma (HEPA-1), and pheochromocytoma (PC-12) cells were relatively resistant to particle injury. While the death pathway in RAW 264.7 cells involves caspase activation, the cytotoxic response in BEAS-2B cells is more necrotic in nature. Using fluorescent-labeled NH(2)-PS, we followed the routes of particle uptake. Confocal microscopy showed that the cationic particles entered a LAMP-1 positive lysosomal compartment in RAW 264.7 cells from where the particles could escape by lysosomal rupture. A proton pump inhibitor interfered in this pathway. Subsequent deposition of the particles in the cytosol induced an increase in mitochondrial Ca(2+) uptake and cell death that could be suppressed by cyclosporin A (CsA). In contrast, NH(2)-PS toxicity in BEAS-2B cells did not involve the LAMP-1 endosomal compartment, stimulation of proton pump activity, or an increase in mitochondrial Ca(2+). Particles were taken up by caveolae, and their toxicity could be disrupted by cholesterol extraction from the surface membrane. Although the particles induced mitochondrial damage and ATP depletion, CsA did not affect cytotoxicity. Cationic particles were taken up into HEPA-1, HMEC, and PC-12 cells, but this did not lead to lysosomal permeabilization, increased Ca(2+) flux, or mitochondrial damage. Taken together, the results of this study demonstrate the importance of cell-specific uptake mechanisms and pathways that could lead to sensitivity or resistance to cationic particle toxicity.
Impedance spectra of tumour tissue in comparison with normal tissue; a possible clinical application for electrical impedance tomography (1996)

Electrical characteristics of living tissues have been investigated for a long time in the search for further methods to complement the traditional investigations of pathology and physiology. Tumour tissue has been shown to exhibit a larger permittivity and conductivity than normal tissues. This might be associated with the fact that tumour cells have a higher water content and sodium concentration than normal cells, as well as different electrochemical properties of their cell membranes. To our knowledge only a few contributions on this subject have been published. This study describes an additional application on measurements of the complex impedance of tumour and normal tissues, in order to compare the impedance features of the two tissue types. The tissue sample is placed in a measuring cell in which the temperature is controlled. The measuring cell is connected to an impedance meter able to measure the complex impedance in terms of real and imaginary part curves for frequencies from 1.5 kHz to 700 kHz. The four-electrode principle is used with the current injected by the outer electrodes and the voltage difference measured between the inner electrodes. The current can be altered up to 1 mA. The instrument can be calibrated with known resistance and capacitance networks connected to the input of the instrument in order to minimize the measurement errors. The calibration routine uses a polynomial adaptation and can be applied interactively. Measurements performed by the instrument show promising results. Preliminary results show that this method can be extended to a new application for detection of tumour tissue by electrical impedance tomography (EIT).
Memory efficient and high-speed search Huffman coding (1995)

Code compression is a key element in high-speed digital data transport. A major compression is performed by converting the fixed-length codes to variable-length codes through a (semi-)entropy coding scheme. Huffman coding is shown to be a very efficient coding scheme. To speed up the process of search for a symbol in a Huffman tree and to reduce the memory size we have proposed a tree clustering algorithm to avoid high sparsity of the tree. The method is shown to be extremely efficient in memory requirement, and fast in searching for the symbol. For an experimental video data with Huffman codes extended up to 13 bits in length, the entire memory space is shown to be 122 words, compared to 2/sup 13/=8192 words in a normal situation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>