gebhart/patrepeval huggingface dataset:
Screening genético preimplantacional (PGS) por factor masculino.: Análisis mediante dos técnicas: FISH y A-CGH (2014)

La indicacion de screening genetico preimplantacional (PGS) por factor masculino de origen genetico ha sido poco estudiada a pesar de suponer el 9% de los casos reportados en el registro de la Sociedad Europea de Reproduccion Humana y Embriologia (ESHRE PGD Consortium). En el presente estudio se han evaluado los resultados de los ciclos de PGS por factor masculino genetico en dos series distintas: una serie de 73 casos en la que se utilizo la tecnica de hibridacion in situ fluorescente (FISH) para el analisis genetico y otra de 55 casos en los que el analisis cromosomico se realizo mediante arrays de hibridacion genomica comparativa (a-CGH). Se ha observado un mayor poder de deteccion de anomalias cromosomicas en el grupo de a-CGH, lo que ha supuesto un porcentaje inferior de ciclos con transferencia en este grupo. No obstante, las tasas de implantacion y embarazo se han visto significativamente mejoradas en el grupo de a-CGH. A dia de hoy, la aplicacion de PGS mediante analisis cromosomico completo (CCS) en el grupo de pacientes con factor masculino genetico ofrece unos resultados muy positivos que validan su aplicacion.
Two tyrosinase nonapeptides recognized on HLA‐A2 melanomas by autologous cytolytic T lymphocytes (1994)

A number of cytolytic T lymphocyte (CTL) clones derived from several melanoma patients have been found to recognize a majority of melanomas from HLA-A2 patients. We have reported previously that two such CTL clones recognize a product of the tyrosinase gene that is presented by HLA-A2. Here we show that one of these CTL clones recognizes a peptide encoded by the first nine amino acids of the putative signal sequence of tyrosinase. The other CTL clone recognizes a different tyrosinase peptide corresponding to amino acids 368-376. Both peptides contain consensus motifs of HLA-A2 binding peptides.
Safe assembly motion - A novel approach for applying human-robot co-operation in hybrid assembly systems (2013)

Task and workspace sharing between human and robots offers an efficient combination of individual strengths and is a promising attempt to meet the continuous increasing demand on flexibility within assembly systems using human-robot co-operation. Here, the major pre-condition is the human safety along robot-based process steps limiting the system effectiveness. Particularly the automated assembly motion implies extensive considerations due to required contacts which are difficult to handle by common safety systems. This paper presents a methodical approach for the continuous model-based supervision of the robot assembly execution in proximate workspace sharing. On this basis, a conceptual framework is derived and the requirements as well as design principles are discussed.
Physiologic mechanisms underlying the failure of the “shock index” as a tool for accurate assessment of patient status during progressive simulated hemorrhage (2013)

The low sensitivity of the SI observed in LT individuals is associated with a lower capacity to withdraw cardiac vagal activity and can lead to an undertriage of those patients most likely to develop early hemorrhagic shock.
Linking gene expression patterns to therapeutic groups in breast cancer. (2000)

A major objective of current cancer research is to develop a detailed molecular characterization of tumor cells and tissues that is linked to clinical information. Toward this end, we have identified approximately one-quarter of all genes that were aberrantly expressed in a breast cancer cell line using differential display. The cancer cells lost the expression of many genes involved in cell adhesion, communication, and maintenance of cell shape, while they gained the expression of many synthetic and metabolic enzymes important for cell proliferation. High-density, membrane-based hybridization arrays were used to study mRNA expression patterns of these genes in cultured cells and archived tumor tissue. Cluster analysis was then used to identify groups of genes, the expression patterns of which correlated with clinical information. Two clusters of genes, represented by p53 and maspin, had expression patterns that strongly associated with estrogen receptor status. A third cluster that included HSP-90 tended to be associated with clinical tumor stage, whereas a forth cluster that included keratin 14 tended to be associated with tumor size. Expression levels of these clinically relevant gene clusters allowed breast tumors to be grouped into distinct categories. Gene expression fingerprints that include these four gene clusters have the potential to improve prognostic accuracy and therapeutic outcomes for breast cancer patients.
Negative Regulation of T Cell Receptor Signaling by Siglec-7 (p70/AIRM) and Siglec-9 (2004)

Siglec-7 (p70/AIRM) and Siglec-9 are "CD33"-related siglecs expressed on natural killer (NK) cells and subsets of peripheral T cells. Like other inhibitory NK cell receptors, they contain immunoglobulin receptor family tyrosine-based inhibitory motifs in their cytoplasmic domains, and Siglec-7 has been demonstrated to negatively regulate NK cell activation. Based on reports of the presence of these siglecs on T cells, we sought to determine if they are capable of modulating T cell receptor (TCR) signaling using Jurkat T cells stably and transiently transfected with Siglec-7 or Siglec-9. Following either pervanadate stimulation or TCR engagement, both Siglecs exhibited increased tyrosine phosphorylation and recruitment of SHP-1. Effects of Siglec-7 and -9 were also evident in downstream events in the signaling pathway. Both siglecs reduced phosphorylation of Tyr319 on ZAP-70, known to play a pivotal role in up-regulation of gene transcription following TCR stimulation. There was also a corresponding decreased transcriptional activity of nuclear factor of activated T cells (NFAT) as determined using a luciferase reporter gene. Like all siglecs, Siglec-7 and -9 recognize sialic acid-containing glycans of glycoproteins and glycolipids as ligands. Mutation of the conserved Arg in the ligand binding site of Siglec-7 (Arg124) or Siglec-9 (Arg120) resulted in reduced inhibitory function in the NFAT/luciferase transcription assay, suggesting that ligand binding is required for optimal inhibition of TCR signaling. The combined results demonstrate that both Siglec-7 and Siglec-9 are capable of negative regulation of TCR signaling and that ligand binding is required for optimal activity.
VEGF‐TRAPR1R2 suppresses choroidal neovascularization and VEGF‐induced breakdown of the blood–retinal barrier (2003)

Vascular endothelial growth factor (VEGF) plays a central role in the development of retinal neovascularization and diabetic macular edema. There is also evidence suggesting that VEGF is an important stimulator for choroidal neovascularization. In this study, we investigated the effect of a specific inhibitor of VEGF, VEGF-TRAP(R1R2), in models for these disease processes. VEGF-TRAP(R1R2) is a fusion protein, which combines ligand binding elements taken from the extracellular domains of VEGF receptors 1 and 2 fused to the Fc portion of IgG1. Subcutaneous injections or a single intravitreous injection of VEGF-TRAP(R1R2) strongly suppressed choroidal neovascularization in mice with laser-induced rupture of Bruch's membrane. Subcutaneous injection of VEGF-TRAP(R1R2) also significantly inhibited subretinal neovascularization in transgenic mice that express VEGF in photoreceptors. In two models of VEGF-induced breakdown of the blood-retinal barrier (BRB), one in which recombinant VEGF is injected into the vitreous cavity and one in which VEGF expression is induced in the retina in transgenic mice, VEGF-TRAP(R1R2) significantly reduced breakdown of the BRB. These data confirm that VEGF is a critical stimulus for the development of choroidal neovascularization and indicate that VEGF-TRAP(R1R2) may provide a new agent for consideration for treatment of patients with choroidal neovascularization and diabetic macular edema.
Unstable optical resonators for laser applications (1965)

A simple geometrical analysis has been developed which describes the lowest-order transverse mode of any large-Fresnel-number optical resonator located in the unstable or high-loss region of the resonator mode chart. Such resonators include, for example, resonators in which one or both of the mirrors are divergent spherical surfaces. The lowest mode in such a resonator is assumed to consist of two oppositely traveling divergent spherical waves which uniformly illuminate the end mirrors. The centers of curvature of these spherical waves do not, in general, coincide with the mirror centers of curvature, but are found by requiring that each center be the image of the other upon reflection from the appropriate mirror. The resonator losses are found from purely geometrical considerations, and are given by simple analytical expressions. These losses turn out to be independent of the mirror sizes, so that hyperbolic universal equiloss contours can be drawn on the resonator mode chart. The losses agree well with more exact results obtained by Fox and Li for a few specific cases. Experimental results in good agreement with the analysis have been obtained using a ruby laser rod having a divergent spherical surface ground directly onto one end of the laser rod. Unstable resonators, particularly the "Cassegrainian" unstable configuration used in the experiments, appear potentially useful for diffraction output coupling applications, and possibly also for transverse mode control, in ruby and other high-gain lasers.
Proper Insertion Angle Is Essential to Prevent Intra‐Articular Protrusion of a Knotless Suture Anchor in Shoulder Rotator Cuff Repair (2009)

The advent of new arthroscopic devices has led to the development of novel techniques of arthroscopic rotator cuff repair. However, failure to recognize specific technical aspects and improper application of these devices can lead to complications. We report a case of intra-articular protrusion of knotless anchors (PEEK PushLock SP, 4.5 x 18.5 mm; Arthrex, Naples, FL), used in the lateral row of a suture-bridging technique for arthroscopic rotator cuff repair. This case draws attention to the increased length of such devices when compared with traditional suture anchors, the technical aspects of proper device use, the possible patient-related factors such as implant-patient size mismatch, and the importance of additional imaging for the investigation of failure to progress postoperatively.
Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms (1994)

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.
Electrical Behavior of Droplet Interface Bilayer Networks:  Experimental Analysis and Modeling (2007)

Aqueous droplets submerged in an oil-lipid mixture become enclosed by a lipid monolayer. The droplets can be connected to form robust networks of droplet interface bilayers (DIBs) with functions such as a biobattery and a light sensor. Such DIB networks might be used as model systems for the study of membrane-based biological phenomena. In this study, we develop and experimentally validate an electrical modeling approach for DIB networks by applying it to describe the current flow through a simple network containing protein pores and blocking molecules. We demonstrate the use of SPICE (Simulation Program with Integrated Circuit Emphasis) for simulating the electrical behavior of DIB networks. The modular and scalable nature of DIB networks should enable a straightforward extension of the analysis presented in this paper to large, complex networks.
The Effect of Physical Exercise After a Concussion: A Systematic Review and Meta-analysis (2017)

Physical exercise appears to improve the PCSS score and symptoms in patients with a concussion. A high-quality RCT evaluating different intensities of exercise at different time points, for different durations after a concussion, for different races/ethnicities, and for sex needs to be conducted to evaluate a clear effect of exercise in patients with a concussion.
Antitumor Activity of Epidermal Growth Factor Receptor–Related Protein Is Mediated by Inactivation of ErbB Receptors and Nuclear Factor-κB in Pancreatic Cancer (2006)

The erbB family of receptor tyrosine kinases plays critical roles in human cancers, including pancreatic cancer. Discovering a specific agent, which targets multiple members of the erbB family, would be important in pancreatic cancer therapy. Recently, we isolated a novel negative regulator of epidermal growth factor receptor (EGFR), termed EGFR-related protein (ERRP), whose expression attenuates EGFR activation. In the current study, we examined the effects of recombinant ERRP on the growth and ligand-induced activation of multiple members of erbB family in three pancreatic cancer cell lines that express varying levels of EGFR and other member(s) of its family, specifically HER-2. Additionally, we compared the growth inhibitory effect of ERRP with that of Erbitux or Herceptin. Our results showed that ERRP is most effective in inhibiting proliferation of BxPC-3, HPAC, and PANC-1 pancreatic cancer cells. ERRP also inhibited ligand-induced activation of EGFR, HER-2, and HER-3 (ErbB3). In contrast, Erbitux and Herceptin only partially or modestly inhibited activation of EGFR, HER-2, and HER-3. Most importantly, ERRP was found to inhibit pancreatic tumor growth in a severe combined immunodeficient mouse xenograft model. The antitumor activity of ERRP correlates well with tumor differentiation and down-regulation of nuclear factor-kappaB activity. In summary, our results suggest that ERRP is an effective pan-erbB inhibitor, which could be a potential therapeutic agent for pancreatic cancers expressing different levels and subclasses of erbB family of proteins.
DE-cadherin-mediated cell adhesion is essential for maintaining somatic stem cells in the Drosophila ovary (2002)

Evidence from many systems has shown that stem cells are maintained in "niches" or specific regulatory microenvironments formed by stromal cells. The question of how stem cells are maintained in their niches is important, and further studies will lead to a better understanding of stem cell regulation and enhance the future use of stem cells in regenerative medicine. Here we show that cadherin-mediated cell adhesion is required for anchoring somatic stem cells (SSCs) to their niches in the Drosophila ovary. DE-cadherin and Armadillo/beta-catenin accumulate in the junctions between SSCs and their neighboring cells, inner germarial sheath cells. Removal of DE-cadherin from SSCs results in stem cell loss in the adult ovary. Furthermore, the cadherin-mediated adhesion is also important for maintaining SSCs in their niches before adulthood. This study provides further support that SSCs are located in a niche formed by their neighboring cells. We have previously shown that DE-cadherin-mediated cell adhesion is essential for anchoring germ-line stem cells to their niches in the Drosophila ovary. This study further implicates cadherin-mediated cell adhesion as a general mechanism for anchoring stem cells to their niches in a variety of systems.
Allosteric modulation of Ras positions Q61 for a direct role in catalysis (2010)

Ras and its effector Raf are key mediators of the Ras/Raf/MEK/ERK signal transduction pathway. Mutants of residue Q61 impair the GTPase activity of Ras and are found prominently in human cancers. Yet the mechanism through which Q61 contributes to catalysis has been elusive. It is thought to position the catalytic water molecule for nucleophilic attack on the gamma-phosphate of GTP. However, we previously solved the structure of Ras from crystals with symmetry of the space group R32 in which switch II is disordered and found that the catalytic water molecule is present. Here we present a structure of wild-type Ras with calcium acetate from the crystallization mother liquor bound at a site remote from the active site and likely near the membrane. This results in a shift in helix 3/loop 7 and a network of H-bonding interactions that propagates across the molecule, culminating in the ordering of switch II and placement of Q61 in the active site in a previously unobserved conformation. This structure suggests a direct catalytic role for Q61 where it interacts with a water molecule that bridges one of the gamma-phosphate oxygen atoms to the hydroxyl group of Y32 to stabilize the transition state of the hydrolysis reaction. We propose that Raf together with the binding of Ca(2+) and a negatively charged group mimicked in our structure by the acetate molecule induces the ordering of switch I and switch II to complete the active site of Ras.
Induction of protective and therapeutic antitumor immunity by a DNA vaccine with a glioma antigen, SOX6 (2008)

We previously reported identifying SOX6 as a glioma antigen by serological screening using a testis cDNA library. Its preferential expression and frequent IgG responses in glioma patients indicate that SOX6 may be a useful target for immunotherapy. To examine whether cytotoxic T-lymphocyte (CTL) responses specific for SOX6 to destroy glioma can be generated in vivo, we treated glioma-bearing mice by vaccination with a plasmid DNA encoding murine full-length SOX6 protein. Following SOX6-DNA vaccination, CTLs specific for SOX6-expressing glioma cells were induced, while normal autologous-cells that had restrictedly expressed SOX6 during embryogenesis were not destroyed. Furthermore, DNA vaccination with SOX6 exerted protective and therapeutic antitumor responses in the glioma-bearing mice. This antitumor activity was abrogated by the depletion of CD4 positive T cells and/or CD8 positive T cells. These results suggest that the SOX6 protein has multiple CTL and helper epitopes to induce antitumor activity and the effectiveness of SOX6-DNA vaccine for the prevention and treatment of glioma.
Environmental Impacts of Cultured Meat Production (2011)

Cultured meat (i.e., meat produced in vitro using tissue engineering techniques) is being developed as a potentially healthier and more efficient alternative to conventional meat. Life cycle assessment (LCA) research method was used for assessing environmental impacts of large-scale cultured meat production. Cyanobacteria hydrolysate was assumed to be used as the nutrient and energy source for muscle cell growth. The results showed that production of 1000 kg cultured meat requires 26-33 GJ energy, 367-521 m(3) water, 190-230 m(2) land, and emits 1900-2240 kg CO(2)-eq GHG emissions. In comparison to conventionally produced European meat, cultured meat involves approximately 7-45% lower energy use (only poultry has lower energy use), 78-96% lower GHG emissions, 99% lower land use, and 82-96% lower water use depending on the product compared. Despite high uncertainty, it is concluded that the overall environmental impacts of cultured meat production are substantially lower than those of conventionally produced meat.
Latrunculins: Novel Marine Toxins That Disrupt Microfilament Organization in Cultured Cells (1983)

Two toxins, latrunculins A and B, which contain a new class of 16- and 14-membered marine macrolides attached to the rare 2-thiazolidinone moiety, were purified recently from the Red Sea sponge Latrunculia magnifica. The effects of these toxins on cultured mouse neuroblastoma and fibroblast cells have been evaluated. In both types of cells, submicromolar toxin concentrations rapidly induce striking changes in cell morphology that are reversible upon removal of the toxin. Immunofluorescence studies with antibodies specific for cytoskeletal proteins reveal that the toxins cause major alterations in the organization of microfilaments without obvious effects on the organization of the microtubular system.
Two DNA Polymerases: HIV Reverse Transcriptase and the Klenow Fragment of Escherichia coli DNA Polymerase I (1993)

The three-dimensional structures of two DNA polymerases are presently known (Ollis et al. 1985; Kohlstaedt et al. 1992). One, the Klenow fragment (KF) of E. coli polymerase I, uses a DNA template whereas the other, HIV reverse transcriptase (RT), can employ either RNA or DNA as a template. As with all DNA polymerases, a primer is required for the initiation of DNA synthesis (for a detailed discussion of polymerases, see Kornberg and Baker 1992). KF initiates DNA synthesis at nicks and gaps in DNA and extends the DNA primer strand on the DNA template. In the case of RT, the first round of DNA synthesis is initiated by a tRNA primer bound to the viral RNA template. This enzyme must be able to recognize tRNA, partially unwind its structure, and facilitate the hydrogen-bonding interaction between the tRNA and the template.
Transmission diversity in wideband CDMA (2003)

We study two transmission diversity techniques in wideband CDMA system. The studied techniques are time switched transmission diversity (TSTD) and selection transmission diversity (STD). In TSTD encoded bits are distributed between all transmission antennas. In STD encoded bits are transmitted through the best transmission antenna. STD requires that a mobile measures which is the best antenna and transmits this information to the base station. We have compared these two methods and a single antenna case and found that TSTD is over 2 dB better and STD 3 dB better than the single antenna case. We have also found that in WCDMA STD can tolerate relatively high amount of feedback signaling errors.
Increased EP4 Receptor Expression in Colorectal Cancer Progression Promotes Cell Growth and Anchorage Independence (2006)

Cyclooxygenase-2 and prostaglandin E(2) (PGE(2)) levels are increased in colorectal cancers and a subset of adenomas. PGE(2) signaling through the EP4 receptor has previously been associated with colorectal tumorigenesis. However, changes in EP4 expression during adenoma to carcinoma progression have not been investigated, neither has whether levels of EP4 influence important markers of malignant potential, such as anchorage-independent growth or the tumors growth response to PGE(2). We report using immunohistochemistry that in vivo EP4 receptor protein expression was increased in colorectal cancers (100%) as well as adenomas (36%) when compared with normal colonic epithelium. EP4 expression was also higher in colorectal carcinoma compared with adenoma cell lines and increased with in vitro models of tumor progression. Adenoma (PC/AA/C1 and RG/C2) and carcinoma cell lines (HT29) were growth stimulated by PGE(2) up to 0.5 micromol/L. However, although carcinoma and transformed adenoma (PC/AA/C1SB10C, a transformed derivative of PC/AA/C1) cells remain stimulated by higher doses of PGE(2) (10 micromol/L), the adenoma cell lines were inhibited. Interestingly, enforced expression of EP4 in the adenoma cell line, RG/C2, resulted in stimulation of growth by 10 micromol/L PGE(2) and promoted anchorage-independent growth. Both in vivo and in vitro data from this study suggest that increased EP4 receptor expression is important during colorectal carcinogenesis. We propose that high levels of PGE(2) in a tumor microenvironment would select for cells with increased EP4 expression, and that the EP4 receptor may therefore represent an important target for colorectal cancer prevention and treatment.
Fabrication and Characterization of Ultrahigh‐Volume‐ Fraction Aligned Carbon Nanotube–Polymer Composites (2008)

Aligned CNT nanocomposites with variable volume fraction, up to 20%, are demonstrated. Biaxial mechanical densification of aligned CNT forests, followed by capillarity-driven wetting using unmodified aerospace-grade polymers, creates centimeter-scale specimens. Characterizations confirm CNT alignment and dispersion in the thermosets, providing a useful platform for controlled nanoscale interaction and nanocomposite property studies that emphasize anisotropy.