gebhart/patrepeval huggingface dataset:
Dual Targeting of Endothelial Cells and Pericytes in Antivascular Therapy for Ovarian Carcinoma (2007)

Strategies targeting both endothelial cells and pericytes are highly effective for in vivo treatment of ovarian carcinoma. This antiangiogenic effect may be partially due to decreased pericyte coverage, thus increasing the sensitivity of tumor vasculature to therapy. These encouraging data support the development of clinical trials based on this strategy.
A Fragile Watermarking Scheme Based on Hash Function for Web Pages (2011)

With the development of Internet, the integrity protection of web pages has become an important problem. A fragile watermarking scheme based on hash function for web pages is proposed in this paper. Watermarks are generated by using hash function and these watermarks are related to the web page's content. Then, the watermarks are embedded into the tags and embedding locations, which are randomly pointed. In the detection phase, regenerated watermarks and extracted watermarks are got from the watermarked web page. Finally, according to comparing the mentioned above two kind watermarks, we can judge whether the web page has been tampered or not. In addition, we can also identify the types of modification and locate the locations of modification.
Prostate Cancer Cells with Stem Cell Characteristics Reconstitute the Original Human Tumor In vivo (2007)

Cancer may arise from a cancer stem/progenitor cell that shares characteristics with its normal counterpart. We report the reconstitution of the original human prostate cancer specimen from epithelial cell lines (termed HPET for human prostate epithelial/hTERT) derived from this sample. These tumors can be described in terms of Gleason score, a classification not applied to any of the transgenic mouse models currently developed to mimic human disease. Immunohistochemical and Western blot analyses indicate that they do not express androgen receptor or p63, similar to that reported for prostate stem cells. These cell lines also express embryonic stem markers (Oct4, Nanog, and Sox2) as well as early progenitor cell markers (CD44 and Nestin) in vitro. Clonally derived HPET cells reconstitute the original human tumor in vivo and differentiate into the three prostate epithelial cell lineages, indicating that they arise from a common stem/progenitor cell. Serial transplantation experiments reconstitute the tumors, suggesting that a fraction of parental or clonally derived HPET cells have self-renewal potential. Thus, this model may enhance our understanding of human tumor development and provide a mechanism for studying cancer stem/progenitor cells in differentiation, tumorigenesis, preclinical testing, and the development of drug resistance.
Antimitogenic effects of interferon and (2′–5′)‐oligoadenylate in synchronized 3T3 fibroblasts (1981)

Interferons are not only antiviral agents, but are also pleiotropic modifiers of cellular functions [ 1,2]. One aspect of IFN’s cellular activity, is its ability to inhibit normal and tumor cell proliferation [3]. In GO-arrested cells stimulated to grow by different mitogens, hormones or other positive growth-factors, IFN was reported to reduce the rate of cell entry into S-phase [4-71. In some cases, it also extended the S t G2 phases of the cell cycle [8,9]. Several observations have led us to propose that the (2’-S’)-oligoadenylate (oligo(A)) synthetase, which is induced in cells by IFN, is involved in the antimitogenic effects of IFN: First, (2’-5’)oligo(A) produces an antimitogenie effect when applied to intact lymphocytes stimulated by concanavalin A [lo]. Second, growthrelated variations in the synthesis and degradation of (2’~S’)-oligo(A) were demonstrated [ 11,121. Here, we have extended the study of the antimitogenic effect of dephosphorylated (2’-5’)ApApA to serum-stimulated Balb/c 3T3 fibroblasts. Using a series of chemically synthesized derivatives of this oligonucleotide, we show that inhibition of DNA synthesis takes place only if the (2’-5’)-oligo(A) is at least 3 nucleotides long and has a free hydroxyl-group at the 5’-position. We show that the antimitogenic action results from a decrease in the rate of cell entry into S-phase. Finally, we present evidence that the antimitogenic activity of (2’-5’)ApApA is mediated by ribonuclease F (RNase F) activation [ 13,141 and a decrease in protein synthesis during the Cl phase of the cell cycle. 2. Materials and methods
SUMO Modification Regulates MafB-Driven Macrophage Differentiation by Enabling Myb-Dependent Transcriptional Repression (2007)

During the execution of differentiation programs, lineage-specific transcription factors are in competition with antagonistic factors that drive progenitor proliferation. Thus, the myeloid transcription factor MafB promotes macrophage differentiation of myeloid progenitors, but a constitutively active Myb transcription factor (v-Myb) can maintain proliferation and block differentiation. Little is known, however, about the regulatory mechanisms that control such competing activities. Here we report that the small ubiquitin-like protein SUMO-1 can modify MafB in vitro and in vivo on lysines 32 and 297. The absence of MafB SUMO modification increased MafB-driven transactivation and macrophage differentiation potential but inhibited cell cycle progression and myeloid progenitor growth. Furthermore, we observed that direct repression of MafB transactivation by v-Myb was strictly dependent on MafB SUMO modification. Consequently, a SUMOylation-deficient MafB K32R K297R (K32,297R) mutant could specify macrophage fate even after activation of inducible Myb alleles and resist their differentiation-inhibiting activity. Our findings suggest that SUMO modification of MafB affects the balance between myeloid progenitor expansion and terminal macrophage differentiation by controlling MafB transactivation capacity and susceptibility to Myb repression. SUMO modification of lineage-specific transcription factors may thus modulate transcription factor antagonism to control tissue homeostasis in the hematopoietic system.
On the thermodynamics of quantum-electrodynamic frequency shifts (1987)

The spectra of atoms exposed to black-body radiation suffer temperature-dependent frequency shifts predicted earlier and now becoming measurable, whose thermodynamic status is clarified. To a good approximation, they are calculable as differences between the coupling-induced shifts in the Helmholtz free energies of the coupled system atom plus quantised electromagnetic field, in thermal equilibrium subject to the constraint that the atom is in a given state li). Effectively, the state label i plays the same role as do macroscopically controlled variables in ordinary thermodynamics. With kT well below the electron rest-mass energy, such calculations require only the forward scattering amplitude of light from the atom; some previously overlooked but theoretically interesting relativistic features of the exact expressions are pointed out. For a harmonic oscillator, uniquely, the constrained free-energy shift is the same for all levels (and therefore also the same as the shift in unconstrained equilibrium); consequently there is no thermal frequency shift at all. Recent comments on black-body-induced Stark shifts by Ford et al. (1985-6) are based on their calculation of the free energy of a harmonic oscillator in unconstrained equilibrium with the field. It is shown that as regards frequency shifts their comments are incompatible with, and misrepresent, previous correct work on such shifts.
Expression, Purification, and Metal Binding Properties of the N-terminal Domain from the Wilson Disease Putative Copper-transporting ATPase (ATP7B) (1997)

The putative copper binding domain from the copper-transporting ATPase implicated in Wilson disease (ATP7B) has been expressed and purified as a fusion to glutathione S-transferase. Immobilized metal ion affinity chromatography revealed that the fusion protein is able to bind to columns charged with different transition metals with varying affinities as follows: Cu(II)>>Zn(II)>Ni(II)>Co(II). The fusion protein did not bind to columns charged with Fe(II) or Fe(III). 65Zinc(II) blotting analysis showed that the domain is able to bind Zn(II) over a range of pH values from 6.5 to 9.0. Competition 65Zn(II) blotting showed that Cd(II), Hg(II), Au(III), and Fe(III) can successfully compete with Zn(II), at comparable concentrations, for binding to the domain. In contrast, the domain had little or no affinity for Ca(II), Mg(II), Mn(II), and Ni(II) relative to copper. Neutron activation analysis of the copper bound to the domain showed a copper:protein ratio of 6.5-7.3:1. Both Cu(II) and Cu(I) were found to have a higher affinity for the domain relative to Zn(II). In addition, a sharp, reproducible transition was only observed in competition experiments with copper, which may suggest that copper binding has some degree of cooperativity.
Oligomerization and Regulated Proteolytic Processing of Angiopoietin-like Protein 4 (2004)

Angiopoietin-like protein 4 (Angptl4) is a recently identified circulating protein expressed primarily in adipose tissue and liver. Also known as peroxisome proliferator-activated receptor (PPAR)-gamma angiopoietin-related, fasting induced adipose factor, and hepatic fibrinogen/angiopoietin-related protein, recombinant Angptl4 causes increase of plasma very low density lipoprotein levels by inhibition of lipoprotein lipase activity. Similar to angiopoietins and other angiopoietin-like proteins, Angptl4 contains an amino-terminal coiled-coil domain and a carboxyl-terminal fibrinogen-like domain. We report here that Angptl4 is evolutionarily conserved among several mammalian species and that full-length Angptl4 protein is an oligomer containing intermolecular disulfide bonds. Oligomerized Angptl4 undergoes proteolytic processing to release its carboxyl fibrinogen-like domain, which circulates as a monomer. Angptl4's N-terminal coiled-coil domain mediates its oligomerization, which by itself is sufficient to form higher order oligomeric structure. Adenovirus-mediated overexpression of Angptl4 in 293 cells shows that conversion of full-length, oligomerized Angptl4 is mediated by a cell-associated protease activity induced by serum. These findings demonstrate a novel property of angiopoietin-like proteins and suggest that oligomerization and proteolytic processing of Angptl4 may regulate its biological activities in vivo.
A Pyrazole Derivative, YM-58483, Potently Inhibits Store-Operated Sustained Ca2+ Influx and IL-2 Production in T Lymphocytes (2003)

In nonexcitable cells, Ca(2+) entry is mediated predominantly through the store depletion-dependent Ca(2+) channels called store-operated Ca(2+) (SOC) or Ca(2+) release-activated Ca(2+) channels. YM-58483, a pyrazole derivative, inhibited an anti-CD3 mAb-induced sustained Ca(2+) influx in acute T cell leukemia, Jurkat cells. But it did not affect an anti-CD3 mAb-induced transient intracellular Ca(2+) increase in Ca(2+)-free medium, nor anti-CD3 mAb-induced phosphorylation of phospholipase Cgamma1. It was suggested that YM-58483 inhibited Ca(2+) influx through SOC channels without affecting the TCR signal transduction cascade. Furthermore, YM-58483 inhibited thapsigargin-induced sustained Ca(2+) influx with an IC(50) value of 100 nM without affecting membrane potential. YM-58483 inhibited by 30-fold the Ca(2+) influx through SOC channels compared with voltage-operated Ca(2+) channels, while econazole inhibited both SOC channels and voltage-operated Ca(2+) channels with an equivalent range of IC(50) values. YM-58483 potently inhibited IL-2 production and NF-AT-driven promoter activity, but not AP-1-driven promoter activity in Jurkat cells. Moreover, this compound inhibited delayed-type hypersensitivity in mice with an ED(50) of 1.1 mg/kg. Therefore, we concluded that YM-58483 was a novel store-operated Ca(2+) entry blocker and a potent immunomodulator, and could be useful for the treatment of autoimmune diseases and chronic inflammation. Furthermore, YM-58483 would be a candidate for the study of capacitative Ca(2+) entry mechanisms through SOC/CRAC channels and for identification of putative Ca(2+) channel genes.
Study of Demodex mites: Challenges and Solutions (2015)

Demodex mites are the largest and most complex organisms of the skin microflora. How they interact with the innate and adaptive immune systems is unknown. Their potential to have a pathogenic role in the causation of human skin disorders causes continued speculation. With growing interest in the microflora of human skin and its relevance to cutaneous health, the role of Demodex mites needs to be better understood. The main challenges facing scientists investigating the role of these organisms and possible solutions are reviewed under the following headings: (1) Determining the mite population in skin, (2) Transporting, extracting and imaging live mites, (3) Maintaining mites viable ex vivo and (4) Establishing methods to determine the immune response to Demodex mites and their internal contents.
Specificity-determining regions of a lepidopteran-specific insecticidal protein produced by Bacillus thuringiensis. (1990)

The lepidopteran-specific, insecticidal crystal proteins of Bacillus thuringiensis vary in toxicity to different species of lepidopteran larvae. We report studies of CryIA(a) and CryIA(c), two related proteins that have different degrees of toxicity to Heliothis virescens yet very similar degrees of toxicity to Manduca sexta. The amino acid differences between these proteins are located primarily between residues 280 and 722. We have constructed a series of chimeric proteins and determined their toxicities to both insects. The most significant findings arise from the replacement of three segments of the cryIA(c) gene with homologous portions of the cryIA(a) gene: codons 332-428, 429-447, and 448-722. Each of these segments contributed substantially and largely additively toward efficacy for H. virescens. However, replacement of the 429-447 segment of cryIA(c) gene with the cryIA(a) sequence resulted in a 27-50-fold reduction in toxicity toward M. sexta whereas the reduction in toxicity to H. virescens was only 3-4-fold. Subdivision of the 429-447 segment and replacements involving residues within this segment reduced toxicity to M. sexta by 5- to more than 2000-fold whereas toxicity to H. virescens was only reduced 3-10-fold. These observations indicate that: 1) different but overlapping regions of the cryIA(c) gene determine specificity to each of the two test insects; 2) some of the examined gene segments interact in determining specificity; and 3) different sequences in the cryIA(a) and cryIA(c) genes are required for maximal toxicity to M. sexta.
Shadow Removal via Flash/Noflash Illumination (2006)

Shadows bedevil multimedia applications, e.g. seeing into shadow regions in surveillance video. Model-based and non-model based, statistical methods, spatial, temporal, and invariant-based methods have been devised for combatting the shadow problem. Here we take a different approach, by attenuating the shadow by utilizing a second image under another illuminant to remove the effect of shadow-edges from an edge map of each frame. As a precursor step, we examine flash/noflash still image pairs. A flash image provides lessened shadows, but other shadows are produced. We can produce a flash-only (no ambient) image by subtracting the two images, but several artifacts remain. Instead, we have used the pure-flash image to detect the ambient shadows [ICME06]. However that method may fail when there are flash-shadows or specularities in the copy region. Here we manipulate the gradient field using a smoothing step including the directionality of edges near the shadow boundary, with improved results
Differential susceptibility to monomeric HIV gp120‐mediated apoptosis in antigen‐activated CD4+ T cell populations (1995)

To support the hypothesis that indirect mechanisms mediated by viral products like the HIV envelope glycoprotein gp120 could be responsible for T lymphocyte depletion in HIV infection, we developed a system in which the impairment of T cell functions could be investigated in vitro. In particular, we characterized the conditions that allow T lymphocytes repeatedly stimulated with an antigen to be sensitive or resistant to gp120-mediated apoptotic signals. To achieve this goal, a panel of antigen-specific CD4+ T cell clones and primary CD4+ T lymphocytes were treated for 2 and 18 h with saturating amounts of monomeric gp120 (without cross-linking with specific antibodies) and antigen-driven T cell proliferation and apoptosis were analyzed. We show that monomeric gp120 induces apoptosis only in T lymphocytes repeatedly stimulated with the antigen, that primary T lymphocytes are resistant to programmed cell death mediated by monomeric gp120, but are sensitive to anti-CD4 antibodies, and that gp120-mediated apoptosis is dependent on the period of time between the binding of gp120 to CD4 and the encounter with antigen. To investigate the different susceptibility to gp120 induced apoptosis of primary CD4+ and T cell clones further, the number of membrane CD4 molecules and their affinity for gp120, together with Bcl-2 and Fas expression, were studied. Our data suggest that a down-modulation of membrane CD4 together with high expression of the Bcl-2 gene and protein characterizes the susceptibility to apoptosis of gp120-treated cells. In conclusion, our results define the phenotypic features of T cells susceptible to HIV gp120-induced apoptosis and demonstrate that the same clonotype, depending on the activation state, may present a differential sensitivity to apoptosis induction.
Metadata: It's not about you after all (2016)

On 18 December 2015, the Administrative Appeals Tribunal held that metadata does not constitute Personal Information under the Privacy Act 1988 (Privacy Act), thereby overturning the Privacy Commissioner's decision that Telstra hand over personal mobile data to those who requested it.
Controlling Polymer-Surface Interactions with Random Copolymer Brushes (1997)

A simple technique for precisely controlling the interfacial energies and wetting behavior of polymers in contact with solid surfaces is described. End-functionalized statistical random copolymers of styrene and methylmethacrylate were synthesized, with the styrene fraction f varying from 0 to 1, and were end-grafted onto silicon substrates to create random copolymer brushes about 5 nanometers thick. For f &lt; 0.7, polystyrene (PS) films (20 nanometers thick) rapidly dewet from the brushes when heated well above the glass transition temperature. The contact angle of the resulting polymer droplets increased monotonically with decreasing f . Similar behavior was observed for poly(methylmethacrylate) (PMMA) films but with an opposite dependence on f . The interfacial energies of the random copolymer brushes with PS and PMMA were equal when f was about 0.6. Thus, precise control of the relative surface affinities of PS and PMMA was possible, demonstrating a way to manipulate polymer-surface interactions.
A guide to Agrobacterium binary Ti vectors (2000)

Agrobacterium-based plasmid vectors allow the transformation of a wide range of plant species by capitalizing on a natural bacterial system to introduce DNA into the nuclear genome of plants. It is often a complex task to consider fully all the possible plasmid vectors and Agrobacterium strains available, and it can thus be difficult to take full advantage of these research tools. This practical guide is a survey of the many binary Ti plasmid vectors and Agrobacterium strains available, and aims to help researchers to make an informed decision about the system that is best suited to their needs...
Immune mechanisms and protective antigens of Vibrio cholerae serogroup O139 as a basis for vaccine development (1996)

We have characterized 11 isolates of Vibrio cholerae O139 Bengal with regard to properties deemed to be relevant for development of a vaccine against O139 cholera. For most strains two colony variants, A and B, which are nonhemolytic and hemolytic, respectively, were detected on blood agar. The A and B variants were associated with high- and low-level production of soluble hemagglutinin-protease, respectively. However, on Luria-Bertani agar both types formed opaque colonies, which has been shown to be associated with capsule formation. Interestingly, under the stationary tube-shaken flask culture conditions in yeast extract-peptone water medium which were used to stimulate the production of cholera toxin (CT) and toxin-coregulated pili, B variants constitutively produced CT and TcpA, two ToxR-regulated proteins, at 28 and 37 degrees C, whereas the production of these proteins by A variants was downregulated at the higher temperature. One of the strains, 4260B, having a well-exposed O antigen and capsule and the capacity to produce large amounts of TcpA, CT, and mannose-sensitive hemagglutinin pili but minimal amounts of the proteolytic soluble hemagglutinin, was selected to produce antibacterial antisera and as a challenge strain in protection studies using the rabbit ileal loop model. Rabbit antisera to live, heat-killed, or formalin-killed O139 vibrios or to purified O139 lipopoly-saccharide (LPS) as well as monoclonal antibodies (MAbs) to O139 LPS agglutinated all O139 isolates. However, when A and B variants of strain 4260 were tested for sensitivity to vibriocidal activity of these antibody preparations, only the B variant was killed. All of the antisera against live or killed O139 vibrios conferred passive protection against fluid accumulation induced by the challenge strain. The protective effects of the antisera were correlated to anti-LPS antibody titers rather than to titers against whole bacteria that had been grown for toxin-coregulated pilus expression. This protection was considerably higher than that conferred by antisera to classical, EI Tor, or recombinantly produced (classical) CT or CTB. Furthermore, MAbs to O139 LPS and CTB-CT exhibited a strong synergistic protection against O139 challenge irrespective of the level of sensitivity of challenge strains to O139 LPS MAbs in vibriocidal assays in vitro.
Telecommunications Network Management into the 21st Century: Techniques, Standards, Technologies, and Applications (1994)

Study of neutrophil phagocytic activity in injuries to the spinal cord and spine and in damage to the spine without involvement of the spinal cord showed that injuries to the spinal cord have no specific effect on this activity. True, the phagocytic reaction is inhibited in the first 2-3 weeks after the trauma both in patients with injury to the spinal cord and spine and in those with injury only to the spine. In later periods after the trauma, however, with the neurological status remaining the same, the values of the phagocytic reaction do not differ from those in healthy persons. The level at which the spinal cord is injured hardly affects the activity of phagocytosis. The examination was conducted in 117 patients with spinal fractures 94 of whom had also sustained injury to the spinal cord.
In vitroandin vivomodels for testing arrhythmogenesis in drugs (2005)

The steadily increasing list of drugs associated with prolongation of the QT interval and torsades de pointes (TdP) constitute a medical problem of major concern. Hence, there is a need at an early stage to identify drug candidates with an inherent capacity to induce repolarization-related proarrhythmias, avoiding exposure of large populations to potentially harmful drugs. Furthermore, the availability of clinically relevant and predictive animal models should reduce the risk that effective and potentially life-saving drugs never reach the market. This review will discuss the pros and cons of some in vivo and in vitro animal models for assessing proarrhythmia liability.