gebhart/patrepeval huggingface dataset:
Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves (2010)

Objectives were to determine effects of feeding pomegranate extract (POMx) rich in polyphenols on performance, health, nutrient digestion, and immunocompetence of calves in the first 70 d of age. Holstein calves (n=67), at 2+/-1 d of age (d 0=birth day) were randomly assigned to 0 (control), 5 (POMx5), or 10 g/d (POMx10) of pomegranate extract containing 16.9% gallic acid equivalent (GAE) to result in intakes of 0, 850 and 1,700 mg of GAE/d or an average of approximately 0, 15, and 30 mg of GAE/kg of body weight (BW) per day. All calves received colostrum during the first 24 h, pasteurized milk thereafter until 61 d of age, and grain was fed ad libitum for the first 70 d of age. Calves were housed in individual hutches, and grain intake, attitude and fecal scores, incidence and duration of health disorders, and treatments for health problems were evaluated daily. Body weight was measured on 2 consecutive days at 2, 30, and 70 d of age and averaged for each measurement. Concentrations of glucose and 3-hydroxybutyrate were measured in plasma. Nutrient digestion was measured using total fecal collection during a 3-d period. Neutrophil phagocytic and killing activities and antibody response to immunization with ovalbumin were measured. Peripheral blood mononuclear cells were cultured and cytokine production measured. Feeding POMx had no effect on intake or BW gain in the first 30 d of age, but after 30 d of age, both grain dry matter intake and BW gain decreased with increasing addition of POMx, which resulted in calves that were 1.8 and 4.3 kg lighter at 70 d of age for POMx5 and POMx10, respectively, compared with controls. Feeding POMx did not influence dry matter, organic matter, or starch digestibility, but it reduced crude protein and fat digestion. Plasma concentrations of glucose and 3-hydroxybutyrate were similar among treatments throughout the first 70 d of age. Measures of calf health such as fecal and attitude scores, risk of fever, and rectal temperature were not altered by treatments. Similarly, neutrophil phagocytic and killing activities did not differ among treatments. On the contrary, feeding POMx increased synthesis of interferon-gamma and interleukin-4 by peripheral blood mononuclear cells and improved total immunoglobulin G responses to ovalbumin vaccination. These results suggest that feeding POMx top-dressed onto the grain suppresses intake of grain and digestibility of fat and protein, likely because of the high tannin content. Nevertheless, polyphenols from POMx enhanced mitogen-induced cytokine production and response to vaccination, which might benefit immune competence of calves and potentially health. Additional studies are warranted to minimize the effect of POMx on intake and digestibility and to better understand the mechanisms by which polyphenols improve immune response of calves.
Interfacing Inorganic Nanowire Arrays and Living Cells for Cellular Function Analysis (2015)

Inorganic nanowires are among the most attractive functional materials, which have emerged in the past two decades. They have demonstrated applications in information technology and energy conversion, but their utility in biological or biomedical research remains relatively under-explored. Although nanowire-based sensors have been frequently reported for biomolecular detection, interfacing nanowire arrays and living mammalian cells for the direct analysis of cellular functions is a very recent endeavor. Cell-penetrating nanowires enabled effective delivery of biomolecules, electrical and optical stimulation and recording of intracellular signals over a long period of time. Non-penetrating, high-density nanowire arrays display rich interactions between the nanostructured substrate and the micro/nanoscale features of cell surfaces. Such interactions enable efficient capture of rare cells including circulating tumor cells and trafficking leukocytes from complex biospecimens. It also serves as a platform for probing cell traction force and neuronal guidance. The most recent advances in the field that exploits nanowire arrays (both penetrating and non-penetrating) to perform rapid analysis of cellular functions potentially for disease diagnosis and monitoring are reviewed.
A Semi‐transparent Plastic Solar Cell Fabricated by a Lamination Process (2008)

An electronic glue-based lamination process combined with interface modification is presented as a one-step process for semitransparent polymer solar cell fabrication. The device is metal-free, semitransparent, flexible, self-encapsulated, and highly efficient, with a maximum external quantum efficiency of 70 % and a power efficiency of 3 % under AM 1.5 global 1 sun solar illumination conditions. This approach represents a critical step towards the ultimate goal of low-cost polymer solar cells.
Characterizing the afferent limb of the baroreflex (2000)

In this study, we develop a model of left ventricular and near-systemic hemodynamics, together with a three-component model of the aortic baroreceptor (BR) using experimental data from rat. The hemodynamic model possesses a third-order Windkessel structure consistent with existing models of human and dog hemodynamics and produces good fits to left ventricular, aortic, and femoral pressure waveforms. The baroreceptor model includes subsystem models of the viscoelastic properties of the aortic wall, converting input pressure to strain impinging on embedded nerve terminal endings; the mechanotransduction properties of baroreceptor terminal endings, converting applied strain to generator potential; and the encoding properties of the first Node of Ranvier, converting generator potential into a train of action potentials (spikes), the frequency of which encodes both mean pressure and rate of change of pressure in the aortic arch.\nThe model mimics the known static and dynamic nonlinearities of the BR, including threshold, saturation, post-excitatory depression, and frequency-dependent hysteresis, using a minimum of parameter adjustment. Because it enables the study of subsystems not easily accessed in an experimental setting, including wall-nerve coupling and mechanotransduction, the model provides an ideal testbed for suggesting detailed ionic mechanisms underlying these behaviors. Overall, the model provides a quantitative description of the mechanical, electrical, and chemical behavior of the sensory nerve and its interaction with the arterial wall in which it is imbedded. Model-generated data agrees quantitatively with single nerve recordings of rat arterial baroreceptors, and qualitatively with data from other species.
Rank 2 intersection types for local definitions and conditional expressions (2003)

We propose a rank 2 intersection type system with new typing rules for local definitions (let-expressions and letrec-expressions) and conditional expressions (if-expressions and match-expressions). This is a further step towards the use of intersection types in "real" programming languages.The technique for typing local definitions relies entirely on the principal typing property (i.e. it does not depend on particulars of rank 2 intersection), so it can be applied to any system with principal typings. The technique for typing conditional expressions, which is based on the idea of introducing metrics on types to "limit the use" of the intersection type constructor in the types assigned to the branches of the conditionals, is instead tailored to rank 2 intersection. However, the underlying idea might also be useful for other type systems.
Continuously Monitored Global Virtual Time. (1997)

Optimistic protocols designed for Parallel Discrete Event Simulation (PDES) rely heavily on the Global Virtual Time (GVT) calculation. Since the simulation uses large amounts of memory, the GVT is used to synchronize processes and discard obsolete system information. In this paper we present a new algorithm, the Continuously Monitored Global Virtual Time (CMGVT). Unlike others, this algorithm allows processes to calculate the GVT based on the local information constantly available to each process. System information, such as the Local Virtual Time (LVT) of each process and information about messages in transit, is appended to simulation messages. We present experimental data that show the performance of the CMGVT algorithm. Keywords: distributed simulation, discrete event simulation, virtual time, parallel processing 1 Introduction Many systems are of a discrete nature. They change their state at discrete points in time due to the occurrence of events [1]. Discrete Event Simulation (D...
Effect of HLA Compatibility on Engraftment of Bone Marrow Transplants in Patients with Leukemia or Lymphoma (1989)

We analyzed the relevance of HLA compatibility to sustained marrow engraftment in 269 patients with hematologic neoplasms who underwent bone marrow transplantations. Each patient received marrow from a family member who shared one HLA haplotype with the patient but differed to a variable degree for the HLA-A, B, and D antigens of the haplotype not shared. These 269 patients were compared with 930 patients who received marrow from siblings with identical HLA genotypes. All patients were treated with cyclophosphamide and total-body irradiation followed by the infusion of unmodified donor marrow cells. The rate of graft failure was 12.3 percent among the recipients of marrow from a donor with only one identical haplotype, as compared with 2.0 percent among recipients of marrow from a sibling with the same HLA genotype (both haplotypes inherited from the same parents) (P less than 0.0001). The incidence of graft failure correlated with the degree of donor HLA incompatibility. Graft failure occurred in 3 of 43 transplants (7 percent) from donors who were phenotypically HLA-matched with their recipient (haplotypes similar, but not inherited from the same parents), in 11 of 121 donors (9 percent) incompatible for one HLA locus, in 18 of 86 (21 percent) incompatible for two loci, and in 1 of 19 (5 percent) incompatible for three loci (P = 0.028). In a multivariate binary logistic regression analysis, independent risk factors associated with graft failure were donor incompatibility for HLA-B and D (relative risk = 2.1; 95 percent confidence interval, 1.7 to 2.5; P = 0.0004) and a positive crossmatch for anti-donor lymphocytotoxic antibody (relative risk = 2.3; 95 percent confidence interval, 1.8 to 2.8; P = 0.0038). Residual host lymphocytes were detected in 11 of 14 patients with graft failure, suggesting that the mechanism for graft failure could be host-mediated immune rejection. We conclude that donor HLA incompatibility and prior alloimmunization are significant risk factors for graft failure, and that a more effective immunosuppressive regimen than those currently used is needed for consistent achievement of sustained engraftment of marrow transplanted from donors who are not HLA-identical siblings.
Characterization and Use of Monoclonal Antibodies for Isolation of Phosphotyrosyl Proteins from Retrovirus-Transformed Cells and Growth Factor-Stimulated Cells (1983)

Protein kinases that phosphorylate the hydroxyl group of tyrosine residues of proteins have been implicated in cell transformation by some retroviruses and in regulation of normal cell growth by some polypeptide growth factors. To facilitate the identification of tyrosine kinase substrates, we developed monoclonal antibodies to the hapten azobenzylphosphonate. One of these antibodies, MA-2G8, proved to be especially attractive in that it bound a derivative of aminophenylphosphate, a close phosphotyrosine analog, with higher affinity than it bound the corresponding derivative of aminobenzylphosphonate; however, its affinity for phosphoserine was negligible. In this paper we describe the optimal conditions for using this antibody to isolate phosphotyrosine proteins, emphasizing particularly that its interaction with phosphotyrosyl proteins is sensitive to ionic detergents and to antibody density on the immunosorbent matrix. The antibody also bound ATP citrate lyase; this enzyme lacks phosphotyrosine but contains phosphohistidine, which is similar structurally to phosphotyrosine. By attaching the antibody at high density to Sepharose beads and omitting ionic detergents from the buffers, it was possible by microbatch immunoadsorption (followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) to isolate the 120,000-dalton transforming protein and several other phosphotyrosyl proteins from cells transformed by Abelson murine leukemia virus. Under the same conditions, phosphotyrosyl proteins were also isolated from human epidermal carcinoma cells (A431) that had been stimulated with epidermal growth factor; most prominent among these proteins was the 170,000-dalton receptor for epidermal growth factor.
A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specific epitope. (1992)

DNA cloned from Chlamydia trachomatis is able to direct the formation of the genus-specific lipopolysaccharide epitope of chlamydiae in enteric Gram-negative bacteria. We now demonstrate that a single C. trachomatis gene (gseA) is sufficient to impart this trait to Escherichia coli. The deduced amino acid sequence of gseA shows 23% identity (66% similarity) to kdtA, an E. coli gene that codes for a bifunctional enzyme catalyzing the addition of two 3-deoxy-D-manno-octulosonic acid (Kdo) residues to lipid A precursors (Clementz, T., and Raetz, C. R. H. (1991) J. Biol. Chem. 266, 9687-9696). Extracts of E. coli expressing gseA transfer at least one additional Kdo unit from CMP-Kdo to precursors already bearing the two Kdo residues attached by the kdtA gene product. Introduction of gseA into an E. coli mutant with a thermolabile kdtA gene product endows cell extracts with the ability to transfer not only the third but also the first two Kdos to lipid A precursors, demonstrating that the C. trachomatis enzyme is at least trifunctional. Given the similarities of these two Kdo transferases and the essentiality of Kdo in Gram-negative bacteria, lipopolysaccharide biosynthesis may be a target for development of novel drugs effective against chlamydiae.
Performance Limitations of a Precision Indoor Positioning System Using a Multi-Carrier Approach (2005)

Dr. David Cyganski is a Professor in the ECE Department at WPI where he performs research and teaches in the areas of linear and non-linear multidimensional signal processing, communications and computer networks, and supervises the WPI Convergent Technology Center. He is an active researcher in the areas of radar imaging, automatic target recognition, machine vision and protocols for computer networks. He is a coauthor of the book Information Technology: Inside and Outside. He has held the administrative positions of Vice President of Information Systems and Vice Provost at WPI.
Power splitters with different output power levels based on directional coupling (2009)

The properties of a wideband photonic crystal power splitter with different output power levels based on directional coupling are investigated numerically with the finite-difference time-domain method. The effects of changing the coupling length and radius of the coupling rods between parallel waveguides on output transmissions are investigated. By further splitting power in each branch a power splitter with four output branches is proposed.
Photosensitized charge separation and hydrogen production in reversed micelle entrapped platinized colloidal cadmium sulphide (1984)

Irradiation of platinized colloidal cadmium sulphide, generated in situ in Aerosol-OT reversed micelle entrapped water pools in iso-octane sensitized water photoreduction by thiophenol, dissolved in the organic phase.
Etiogenic factors present in the cerebrospinal fluid from amyotrophic lateral sclerosis patients induce predominantly pro-inflammatory responses in microglia (2017)

Our findings demonstrate that the cerebrospinal fluid from amyotrophic lateral sclerosis patients holds enough cues to induce microglial inflammatory processes as an early event, which may contribute to the neurodegeneration seen in the sporadic amyotrophic lateral sclerosis. These findings highlight the dynamic role of microglial cells in the pathogenesis of the disease, thus suggesting the need for a multidimensional and temporally guarded therapeutic approach targeting the inflammatory pathways for its treatment.
Polyhedral Silver Nanocrystals with Distinct Scattering Signatures (2006)

A scattering of silver: Polyhedral silver nanocrystals display complex and distinct scattering signatures dictated by their shape and size (see picture). The ability to engineer specific plasmon modes should have profound consequences for surface-enhanced Raman spectroscopy, subwavelength optics, and plasmonic transport.
Molecular analysis of chromosome 11 deletions in aniridia-Wilms tumor syndrome. (1985)

We describe five individuals who have constitutional deletions of the short arm of one chromosome 11, including all or part of the band p13. All of these individuals suffer from aniridia; two have had a Wilms tumor removed. We have established lymphoblastoid cell lines from these and in three cases constructed somatic cell hybrids containing the deleted chromosome 11. Analysis of DNA from the cell lines and hybrids with a cloned cDNA probe has shown that the catalase gene is deleted in four of five patients. The catalase locus must be proximal to the Wilms and aniridia-related loci. We have not detected a deletion of the beta-globin or calcitonin genes in any of these individuals; we conclude these genes are likely to be outside the region 11p12-11p15.4. In addition, we have used monoclonal antibodies in fluorescence-activated cell sorting analysis to measure expression in the hybrids of two cell surface markers encoded by genes that map to the short arm of chromosome 11. The genes for both of these are deleted in two individuals but are present in the individual with the smallest deletion.
Acetylation of Histones and Transcription-Related Factors (2000)

The state of chromatin (the packaging of DNA in eukaryotes) has long been recognized to have major effects on levels of gene expression, and numerous chromatin-altering strategies-including ATP-dependent remodeling and histone modification-are employed in the cell to bring about transcriptional regulation. Of these, histone acetylation is one of the best characterized, as recent years have seen the identification and further study of many histone acetyltransferase (HAT) proteins and their associated complexes. Interestingly, most of these proteins were previously shown to have coactivator or other transcription-related functions. Confirmed and putative HAT proteins have been identified from various organisms from yeast to humans, and they include Gcn5-related N-acetyltransferase (GNAT) superfamily members Gcn5, PCAF, Elp3, Hpa2, and Hat1: MYST proteins Sas2, Sas3, Esa1, MOF, Tip60, MOZ, MORF, and HBO1; global coactivators p300 and CREB-binding protein; nuclear receptor coactivators SRC-1, ACTR, and TIF2; TATA-binding protein-associated factor TAF(II)250 and its homologs; and subunits of RNA polymerase III general factor TFIIIC. The acetylation and transcriptional functions of these HATs and the native complexes containing them (such as yeast SAGA, NuA4, and possibly analogous human complexes) are discussed. In addition, some of these HATs are also known to modify certain nonhistone transcription-related proteins, including high-mobility-group chromatin proteins, activators such as p53, coactivators, and general factors. Thus, we also detail these known factor acetyltransferase (FAT) substrates and the demonstrated or potential roles of their acetylation in transcriptional processes.
Disruption of the Murine Lecithin:Cholesterol Acyltransferase Gene Causes Impairment of Adrenal Lipid Delivery and Up-regulation of Scavenger Receptor Class B Type I (1997)

Lecithin:cholesterol acyltransferase (LCAT) is the major determinant of the cholesteryl ester (CE) content of high density lipoprotein (HDL) in plasma. The selective uptake of HDL-CE is postulated to participate in delivery of tissue-derived cholesterol both to the liver and steroidogenic tissues. Recent studies comparing mice with similarly low levels of HDL, due to the absence of either of the two major HDL-associated apolipoproteins apoA-I and apoA-II, suggest that apoA-I is crucial in modulating this process, possibly through interaction with scavenger receptor class B type I (SR-BI). Because of the central role of LCAT in determining the size, lipid composition, and plasma concentration of HDL, we have created LCAT-deficient mice by gene targeting to examine the effect of LCAT deficiency on HDL structure and composition and adrenal cholesterol delivery. The HDL in the LCAT-deficient mice was reduced in its plasma concentration (92%) and CE content (96%). The HDL particles were heterogeneous in size and morphology and included numerous discoidal particles, mimicking those observed in LCAT-deficient humans. The adrenals of the male Lcat (-/-) mice were severely depleted of lipid stores, which was associated with a 2-fold up-regulation of the adrenal SR-BI mRNA. These studies demonstrate that LCAT deficiency, similar to apoA-I deficiency, is associated with a marked decrease in adrenal cholesterol delivery and supports the hypothesis that adrenal SR-BI expression is regulated by the adrenal cholesterol.
The acoustic emission technique in orthopaedics - a review (2004)

Traditionally, orthopaedic research has focused upon the assessment of individual components in an implanted construct. A great deal of research has investigated the durability of the metallic stem, even though this is the most robust component in the implanted system. Standards have been developed for the structural assessment of the implant and even the bone cement which holds the implant in place in the bone. However, the construct as a whole, and its short- and long-term structural integrity, are rarely assessed, and few methods have been established to monitor and predict the mechanisms leading to failure. These are necessary to ensure that any new implants entering the market will perform satisfactorily and prevent premature revision surgery. The acoustic emission (AE) technique offers the capability of monitoring structural degradation passively and in real time, and can distinguish failure mechanisms and their location through the analysis of AE parameters. In the present paper, the use of acoustic emission in orthopaedics, in particular for the evaluation of hip replacement constructs, is reviewed. Following this, three case studies undertaken at the University of Southampton are presented, in which acoustic emission on-line monitoring has been used to evaluate the performance of simulated artificial hip replacement constructs and their constituents during static and fatigue testing. In Case Study 1, the fatigue behaviour of bone cement is characterized; in Case Study 2, the residual stresses induced in the construct as a result of bone cement cure are investigated; in Case Study 3, the mechanisms leading to failure of a carbon fibre reinforced plastic hip stem during fatigue testing are characterized.