gebhart/patrepeval huggingface dataset:
Specification of distinct motor neuron identities by the singular activities of individual Hox genes (1999)

Hox genes have been implicated in specifying positional values along the anteroposterior axis of the caudal central nervous system, but their nested and overlapping expression has complicated the understanding of how they confer specific neural identity. We have employed a direct gain-of-function approach using retroviral vectors to misexpress Hoxa2 and Hoxb1 outside of the normal Hox expression domains, thereby avoiding complications resulting from possible interactions with endogenous Hox genes. Misexpression of either Hoxa2 or Hoxb1 in the anteriormost hindbrain (rhombomere1, r1) leads to the generation of motor neurons in this territory, even though it is normally devoid of this cell type. These ectopic neurons have the specific identity of branchiomotor neurons and, in the case of Hoxb1-induced cells, their axons leave the hindbrain either by fasciculating with the resident cranial motor axons at isthmic (trochlear) or r2 (trigeminal) levels of the axis or via novel ectopic exit points in r1. Next, we have attempted to identify the precise branchiomotor subtypes that are generated after misexpression and our results suggest that the ectopic motor neurons generated following Hoxa2 misexpression are trigeminal-like, while those generated following Hoxb1 misexpression are facial-like. Our data demonstrate, therefore, that at least to a certain extent and for certain cell types, the singular activities of individual Hox genes (compared to a combinatorial mode of action, for example) are sufficient to impose on neuronal precursor cells the competence to generate distinctly specified cell types. Moreover, as these particular motor neuron subtypes are normally generated in the most anterior domains of Hoxa2 and Hoxb1 expression, respectively, our data support the idea that the main site of individual Hox gene action is in the anteriormost subdomain of their expression, consistent with the phenomenon of posterior dominance.
Symptomatic and neuroprotective effects following activation of nigral group III metabotropic glutamate receptors in rodent models of Parkinson's disease (2010)

These findings highlight group III mGlu receptors in the SN as potential targets for providing both symptomatic and neuroprotective relief in PD, and indicate that inhibition of glutamate release in the SN may underlie these effects.
Evidence of Oxidative Stress in Human Corneal Diseases (2002)

This study localized malondialdehyde (MDA, a toxic byproduct of lipid peroxidation), nitrotyrosine [NT, a cytotoxic byproduct of nitric oxide (NO)], and nitric oxide synthase isomers (NOS) in normal and diseased human corneas. Normal corneas (n=11) and those with clinical and histopathological diagnoses of keratoconus (n=26), bullous keratopathy (n=17), and Fuchs' endothelial dystrophy (n=12) were examined with antibodies specific for MDA, NT, eNOS (constitutive NOS), and iNOS (inducible NOS). Normal corneas showed little or no staining for MDA, NT, or iNOS, whereas eNOS was detected in the epithelium and endothelium. MDA was present in all disease groups, with each group displaying a distinct pattern of staining. NT was detected in all keratoconus and approximately one half of Fuchs' dystrophy corneas. iNOS and eNOS were evident in all the diseased corneas. Keratoconus corneas showed evidence of oxidative damage from cytotoxic byproducts generated by lipid peroxidation and the NO pathway. Bullous keratopathy corneas displayed byproducts of lipid peroxidation but not peroxynitrite (MDA but not NT). Conversely, Fuchs' dystrophy corneas displayed byproducts of peroxynitrite with little lipid peroxidation (NT >> MDA). These data suggest that oxidative damage occurs within each group of diseased corneas. However, each disease exhibits a distinctive profile, with only keratoconus showing prominent staining for both nitrotyrosine and MDA. These results suggest that keratoconus corneas do not process reactive oxygen species in a normal manner, which may play a major role in the pathogenesis of this disease.
Catalytic Amplification of the Soft Lithographic Patterning of Si. Nonelectrochemical Orthogonal Fabrication of Photoluminescent Porous Si Pixel Arrays (2001)

Photoluminescent, porous silicon pixel arrays were fabricated via a Pt-promoted wet etching of p-type Si(100) using a 1:1:1 EtOH/HF/H2O2 solution. The pixels were fabricated with micrometer-scale design rules on a silicon substrate that had been modified with an octadecyltrichlorosilane (OTS) monolayer patterned using microcontact printing. The printed OTS layer serves as an orthogonal resist template for the deposition of a Pt(0) complex, which preferentially deposits metal species in areas not covered with OTS. The Pt centers generate a localized oxidative dissolution process that pits the Si in the Pt-coated regions, resulting in the formation of a porous silicon microstructure that luminesces around 580 nm upon illumination with a UV source. Scanning electron microscopy and fluorescence microscopy images of the fabricated porous silicon structures showed that features in the size range of approximately 10-150 microm, and possibly smaller, can be generated by this catalytically amplified soft lithographic patterning method. Importantly, the OTS acts as an etch mask, so that, even with significant hole transport, etching is confined to areas coated with the Pt(0) complex.
Pipelined Asynchronous Circuits (1998)

This thesis presents a design style for implementing communicating sequential processes (CSP) as quasi delay insensitive asynchronous circuits, based on the compilation method of [1]. Although hand compilation can always yield optimal circuits to a good designer, a restricted approach is suggested which can easily implement circuits with some slack between inputs and outputs. These circuits are fast and versatile building blocks for highly pipelined designs. The first chapter presents the implementation approach for individual cells. The second chapter investigates the time behavior of complex pipelined circuits, with the goal of adding slack where necessary and adjusting transistor sizes to optimize the overall throughput.
Inositol‐requiring 1/X‐box‐binding protein 1 is a regulatory hub that links endoplasmic reticulum homeostasis with innate immunity and metabolism (2010)

Inositol-requiring 1 (IRE1)/X-box-binding protein 1 (XBP1)-mediated signalling represents the most conserved branch of the unfolded protein response. A series of recent studies reveal novel and potentially ancient roles for this pathway in the coordination of metabolic and immune responses.
The Application of Near Infrared Spectroscopy in Wheat Quality Control (2012)

No requirement for the use of chemicals and their preparation,
Semantic Similarity in a Taxonomy: An Information-Based Measure and its Application to Problems of Ambiguity in Natural Language (1999)

This article presents a measure of semantic similarity in an IS-A taxonomy based on the notion of shared information content. Experimental evaluation against a benchmark set of human similarity judgments demonstrates that the measure performs better than the traditional edge-counting approach. The article presents algorithms that take advantage of taxonomic similarity in resolving syntactic and semantic ambiguity, along with experimental results demonstrating their effectiveness.
Comparative studies on pharmacokinetics and metabolism of the anti‐juvenile hormone precocene II (1985)

Abstract The cuticular penetration and pharmacokinetics of the anti‐juvenile hormone precocene II were determined in a sensitive species ( Oncopeltus fasciatus ) and an insensitive species ( Heliothis zea ). Precocene was sequestered by the fat body and slowly metabolized in Oncopeltus , but rapidly metabolized and excreted in Heliothis . Studies in vitro using inhibitors for cytochrome P‐450 and for cyt P‐450‐NADPH‐reductase, confirmed the anticipated detoxification of precocene by a mixed‐function oxidase via the 3,4‐epoxide. Use of the inhibitors in vivo had no influence on the metabolism of precocene.
Inter-Vehicle Communication Protocol for Cooperatively Capturing and Sharing Intersection Video (2010)

For accident prevention at intersections, it is useful for drivers to grasp the position of vehicles in blind spots. This can be achieved without infrastructure if some vehicles passing near the intersection capture and share live video of the intersection through inter-vehicle communications. However, such video streaming requires a congestion control mechanism. In this paper, aiming to let a driver grasp the situation at an intersection, we propose a method to select vehicles that send a video in order to generate a live bird's-eye-view video of the intersection. In our method, each vehicle at an intersection exchanges information with others, such as the sub-areas of the intersection it captures, the quality of its video, and its position and speed. Based on the exchanged information, each vehicle autonomously judges whether it should send its video or not. Through simulation with a QualNet simulator, we confirm that our method achieves a good video arrival rate and video quality sufficient for practical use.
Low-temperature Si and Si:Ge epitaxy by ultrahigh-vacuum/chemical vapor deposition: Process fundamentals (1990)

This paper is an overview of work at the IBM Thomas J. Watson Research Center on the chemical and physical considerations underlying the development of a low-temperature chemical vapor deposition process, designated ultra high-vacuum/chemical vapor deposition (UHV/CVD). The origins of the rigorous vacuum and chemical purity requirements of the process are discussed. Operating in the range of 500°C, the process has made it possible to explore the use, in silicon-based devices and atomic-length-scale structures, of a number of metastable materials in the Si:Ge:B system. Also discussed is associated experimental work on the fabrication of high-speed heterojunction bipolar transistors and high-mobility two-dimensional hole-gas structures.
Three-dimensional reservoir characterization and flow simulation of heterolithic tidal sandstones (2005)

Tidal sandstone reservoirs contain significant intervals of hydrocarbon-bearing heterolithic facies, characterized by the presence of tide-generated sedimentary structures such as flaser, wavy, and lenticular bedding (millimeter to centimeter sand-mud alternations). We have characterized the reservoir properties (sandstone connectivity, effective permeability, and displacement efficiency) of these facies using three-dimensional (3-D) models reconstructed directly from large rock specimens. The models are significantly larger than a core plug, but smaller than a typical reservoir model grid block. We find that the key control on reservoir quality is the connectivity and continuity of the sandstone and mudstone layers. If the sandstone layers form a connected network, they are likely to be productive even at low values of net-to-gross (about 0.30.5). This may explain why the productivity of low net-to-gross, heterolithic tidal sandstones is commonly underestimated or overlooked. Connectivity is the dominant control on the transition between productive (pay) and nonproductive (nonpay) heterolithic facies. However, connectivity is difficult to characterize because core plugs sampled from the subsurface are too small to capture connectivity, whereas two-dimensional outcrop measurements can significantly underestimate the true 3-D value. Our results suggest that core-plug measurements of permeability and displacement efficiency are unlikely to yield representative values at the scale of a reservoir model grid block because the connectivity and continuity of sandstone and mudstone layers varies significantly with length scale.
Lipid peroxidation is essential for α‐synuclein‐induced cell death (2015)

Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated α-synuclein (α-Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric α-Syn on one of the major markers of oxidative stress, lipid peroxidation, in primary co-cultures of neurons and astrocytes. We found that oligomeric but not monomeric α-Syn significantly increases the rate of production of reactive oxygen species, subsequently inducing lipid peroxidation in both neurons and astrocytes. Pre-incubation of cells with isotope-reinforced polyunsaturated fatty acids (D-PUFAs) completely prevented the effect of oligomeric α-Syn on lipid peroxidation. Inhibition of lipid peroxidation with D-PUFAs further protected cells from cell death induced by oligomeric α-Syn. Thus, lipid peroxidation induced by misfolding of α-Syn may play an important role in the cellular mechanism of neuronal cell loss in Parkinson's disease. We have found that aggregated α-synuclein-induced production of reactive oxygen species (ROS) that subsequently stimulates lipid peroxidation and cell death in neurons and astrocytes. Specific inhibition of lipid peroxidation by incubation with reinforced polyunsaturated fatty acids (D-PUFAs) completely prevented the effect of α-synuclein on lipid peroxidation and cell death.
Microwave imaging for neoadjuvant chemotherapy monitoring (2006)

We have developed a clinical microwave imaging system for breast imaging applications including cancer detection and monitoring of neoadjuvant chemotherapy. For the latter, key features of any montoring system must include the ability to image a target in a repeatable fashion while being noninvasive and relatively inexpensive. Our microwave imaging system is particularly useful in this setting because we have overcome challenges such as the use of a priori information with the combination of our unique imaging configuration and software. The hardware incorporates monopole antennas which present isotropically radiated fields within which complex nulls (and accompanying non-unique signal phase distributions) are generally not generated until the electromagnetic wave has propagated beyond the illumination zone and associated receiver antennas. This is particularly useful for our variance stabilizing transformation algorithm which can recover accurate dielectric property maps without a priori information or converging to local minima as long as the unwrapped phase distribution remains unique. The associated unwrapping of the measurement data can be readily performed through the use of multi-frequency data and standard unwrapping algorithms.
The IκB Kinase (IKK) Inhibitor, NEMO-binding Domain Peptide, Blocks Osteoclastogenesis and Bone Erosion in Inflammatory Arthritis (2004)

Activation of NF-kappaB leads to expression of ample genes that regulate inflammatory and osteoclastogenic responses. The process is facilitated by induction of IkappaB kinase (IKK) complex that phosphorylates IkappaB and leads to its dissociation from the NF-kappaB complex, thus permitting activation of NF-kappaB. The IKK complex contains primarily IKKalpha, IKKbeta, and the regulatory kinase IKKgamma, also known as NEMO. NEMO regulates the IKK complex activity through its binding to carboxyl-terminal region of IKKalpha and IKKbeta, termed NEMO-binding domain (NBD). In this regard, a cell-permeable NBD peptide has been shown to block association of NEMO with the IKK complex and inhibit activation of NF-kappaB. Given the pivotal role of cytokine-induced NF-kappaB in osteoclastogenesis and inflammatory bone loss, we deduced that cell-permeable TAT-NBD peptide may hinder osteoclastogenesis and bone erosion in inflammatory arthritis. Using NBD peptides, we show that wild type, but not mutant, NBD blocks IKK activation and reduces cytokine-induced promoter and DNA binding activities of NF-kappaB and inhibits cytokine-induced osteoclast formation by osteoclast precursors. Consistent with the key role of NF-kappaB in osteoinflammatory responses in vivo, wild type TAT-NBD administered into mice prior to induction of inflammatory arthritis efficiently block in vivo osteoclastogenesis, inhibits focal bone erosion, and ameliorates inflammatory responses in the joints of arthritic mice. The mutant NBD peptide fails to exert these functions. These results provide strong evidence that IKKs are potent regulators of cytokine-induced osteoclastogenesis and inflammatory arthritis. More importantly, blockade of NEMO assembly with the IKK complex is a viable strategy to avert inflammatory osteolysis.
Improvement of Transient Response of Thermal Power Plant Using VVVF Inverter (2007)

The construction of a thermal power plant is relatively little. However, the thermal power plant holds approximately 65% of world power source. Recently, the concern with the request of improvement for deterioration in the efficiency has been growing in the existing thermal power plant. Especially, it is required the efficiency for a small thermal power plant that is operated in the partial load. An inverter power source is applied to the field of various power electronics for the energy saving. When the inverter power source occurs abnormally, what is at stake is time delay in the transient response of air flow controller to the heating balance of the boiler and NO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">X</sub> of environment factor. Therefore, the purpose of this paper is to present a new air flow control to improve of transient response in partial load for safety operation in the boiler when the inverter of forced draft fan is occurred abnormally. As a result, the combustion remained gas oxygen is back to original condition less than 1.0 second in the all case.
P16INK4a expression adenovirus vector to suppress pancreas cancer cell proliferation. (1999)

The prognoses of pancreatic cancer patients have been miserable even after radical surgery, and adjuvant therapy is necessary to improve the surgical results. p16(INK4a) (p16) is tight-binding and inhibitory protein for cyclin-dependent kinase 4 to induce G1 arrest of the cell cycle. p16 gene deletion is frequently identified in human pancreas cancer. The impaired gene function of p16 might be a major factor of the uncontrolled proliferation and malignancy of pancreas cancer cells. In this study, we investigated the effect of adenovirus p16 expression vector for pancreas cancer cell proliferation to clarify whether the vector might be a promising mode to assist the surgical therapy for pancreas cancer. We constructed the adenovirus p16 expression vector AdexCACSp16 by inserting p16 cDNA to a cassette cosmid containing a nearly full-length adenovirus type 5 genome with E1 and E3 deletions. Thereafter, we assessed the activity of AdexCACSp16 to induce p16 gene mRNA expression in pancreas cancer cell line MIAPaCa-2 and to control cell proliferation. AdexCACSp16 induced a high level of p16 gene mRNA expression in MIAPaCa-2 cells with 1 h contact to the cells. The cell proliferation was significantly suppressed by AdexCACSp16 compared with the control adenovirus group. These data indicate that AdexCACSp16 has the potential to induce p16 gene expression and control pancreas cancer cell proliferation and that the adenovirus p16 expression vector AdexCACSp16 might be a possible method of gene therapy to improve the surgical therapeutic results for pancreas cancer.
El testimonio de las imágenes (2000)

Els historiadors són cada cop més conscients del valor com a documents que tenen les imatges del passat. Alguns han parlar fins i tot d'un gir visual en els estudis històrics comparable al que ha estat el gir lingüístic a les darreres dècades. Determinats àmbits, com ara la cultura política, resulten especialment adients per fer-ho. El consens però, a penes s'ha traduït en investigacions concretes basades en dades visuals. La principal raó de la distància entre les intencions i els resultats podria trobar-se en la manca d'una epistemologia adequada. La major part dels historiadors en actiu han estat formats per treballar amb textos escrits però estan mancats de la instrucció necessària per llegir documents visuals.
Chemically defined polyethylene glycol siRNA conjugates with enhanced gene silencing effect (2014)

The therapeutic application of siRNA suffers from poor bioavailability caused by rapid degradation and elimination. The covalent attachment of PEG is a universal concept to increase molecular size and enhance the pharmacokinetic properties of biomacromolecules. We devised a facile approach for attachment of PEG molecules with a defined molecular weight, and successful purification of the resulting conjugates. We directly conjugated structurally defined PEG chains with twelve ethylene glycol units to the 3'-terminal hydroxyl group of both sense and antisense strands via an aminoalkyl linker. The conjugates were easily purified by HPLC and successful PEGylation and molecule integrity were confirmed by ESI-MS. The evaluation of in vitro gene knockdown of two different targets in MCF-7 breast cancer cells showed stable pharmacologic activity when combined with a standard transfection reagent. Sense strand PEGylation even increased the silencing potency of a CRCX4-siRNA which had modest activity in its wild-type form. The results indicate that PEG chains at the 3'-terminus of both strands of siRNA are well tolerated by the RNAi effector. The attachment of short, chemically defined PEG chains is a feasible approach to improve the pharmacokinetic properties of siRNA, and can be combined with other targeted and untargeted delivery vehicles.
IgG subclass response to HIV in relation to antibody-dependent cellular cytotoxicity at different clinical stages. (1988)

The anti-HIV IgG subclass response was analysed in sera from different clinical stages and related to virus specific antibody-dependent cellular cytotoxicity (ADCC). IgG1 was found to be the dominant subclass, present in all sera and with similar mean titres at different stages. The number of anti-HIV IgG3 positive sera, measured on whole viral lysate antigen plates, decreased during disease progression from 38% in symptom-free to 7% in AIDS patients. IgG2 and IgG4 subclasses were less prevalent although a slight increase of IgG4 frequency was found in AIDS patients. High IgG1 titres correlated with a positive ADCC reaction but there was no correlation between anti-HIV IgG1 and ADCC titres. Some sera which contained HIV IgG1 as the only subclass were able to mediate an ADCC reaction. In addition, when anti-HIV IgG3 was isolated, by protein A chromatography, no ADCC killing was induced by these antibodies. It is concluded that IgG1 is the major ADCC-active IgG subclass in HIV infected individuals. The lack of correlation between IgG1 and ADCC titres may be explained by a relatively small fraction of IgG1 antibodies mediating ADCC.
Improved Efficacy of αvβ3-Targeted Albumin Conjugates by Conjugation of a Novel Auristatin Derivative (2007)

Cellular handling of drug delivery preparations en route to the lysosomal compartment has been extensively studied, but little is known about cellular handling of drugs subsequent to their release from the delivery system. We studied a series of closely related drug targeting conjugates, consisting of albumins equipped with alpha vbeta 3-selective RGD-peptide homing ligands, PEG stealth domains, and either the antitubulin agent monomethyl auristatin E (MMAE) or a new F-variant (MMAF). Since MMAF has a C-terminal charge, this compound is potentially less prone to passive redistribution after its release from the carrier. We demonstrate that RGD-peptide-equipped albumin conjugates with MMAF were indeed more potent than MMAE conjugates, in killing both alpha vbeta 3-positive tumor cells and proliferating endothelial cells. Efficacy increased more in tumor cells than in endothelial cells, suggesting different drug redistribution behavior for the two cell types. Binding affinity and uptake of the conjugate and the cellular handling of released drug contributed to the final efficacy of drug-carrier conjugates, highlighting the importance of all aspects to be carefully considered in the design of targeted drug delivery preparations.