gebhart/patrepeval huggingface dataset:
Dielectrophoretic Trapping of Single Bacteria at Carbon Nanofiber Nanoelectrode Arrays (2007)

We present an ac dielectrophoretic (DEP) technique for single-cell trapping using embedded carbon nanofiber (CNF) nanoelectrode arrays (NEAs). NEAs fabricated by inlaying vertically aligned carbon nanofibers in SiO2 matrix are applied as "points-and-lid" DEP devices in aqueous solution. The miniaturization of the electrode size provides a highly focused electrical field with the gradient enhanced by orders of magnitude. This generates extremely large positive DEP forces near the electrode surface and traps small bioparticles against strong hydrodynamic forces. This technology promises new capabilities to perform novel cell biology experiments at the nanoscale. We anticipate that the bottom-up approach of such nano-DEP devices allows the integration of millions of nanolectrodes deterministically in lab-on-a-chip devices and will be generally useful for manipulating submicron particles.
Nonlinear dynamics in neural computation. (2006)

Nutritional potential of crops was evaluated from the viewpoint of selected vitamins and antioxidants to create a well-balanced diet. Combined use of both traditional (wheat, barley) and underutilized crops (quinoa) is recommended. HPLC methods and image analysis were successfully used as viable tools for food quality determination.
Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. (1980)

The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, proliferates continuously in suspension culture and consists predominantly (greater than 90%) of promyelocytes. These cells can be induced to differentiate to morphologically and functionally mature granulocytes by incubation with a wide variety of compounds, including butyrate and hypoxanthine and polar planar compounds such as dimethyl sulfoxide and hexamethylene bisacetamide. We have now found that retinoic acid (all-trans-retinoic acid) induces differentiation (as measured morphologically and by the ability to reduce nitroblue tetrazolium) of HL-60 at concentrations as low as 1 nM. Maximal differentiation (approximately 90%) occurs at 1 micro M, a concentration 1/500th to 1/160,000th the concentrations of butyrate (0.5 mM) and dimethyl sulfoxide (160 mM) that promote a similar increase in differentiation. Continuous exposure to retinoic acid is necessary for optimal differentiation, with the percentage of mature cells in the culture directly related to the length of time of exposure to retinoic acid. Retinoic acid and 13-cis-retinoic acid are equally effective in inducing differentiation of HL-60. Retinol (vitamin A), retinal, and retinyl acetate are approximately 1/1000th less potent. This study suggests that retinoids could provide a therapeutic tool in the treatment of acute myeloid leukemia, a disease that has been looked upon as primarily involving a block in myeloid differentiation, and indicates that retinoids, in addition to their well-characterized involvement in epithelial cell differentiation, may also be involved in the differentiation of certain hematopoietic cells.
A Candidate Gene Approach Identifies the TRAF1/C5 Region as a Risk Factor for Rheumatoid Arthritis (2007)

Using a candidate-gene approach we have identified a novel genetic risk factor for RA. Our findings indicate that a polymorphism in the TRAF1/C5 region increases the susceptibility to and severity of RA, possibly by influencing the structure, function, and/or expression levels of TRAF1 and/or C5.
不走寻常路——用想象力加一点点技巧，打造属于你自己的电子贺卡 (2004)

又到了一年中最热闹的时候。这几个接踵而至的西方节日大有喧宾夺主之势，看看每年圣诞节商家铺天盖地的促销活动和浓妆淡抹的商业街道就能发现——春节的宣传攻势也不过如此。相比之下，Halloween和Thanksgiving等节日并未大行其道，我们更不会像老美那样忙活着张灯结彩大开Party，
Induction of Synaptic Potentiation in Hippocampus by Patterned Stimulation Involves Two Events (1986)

Electrical stimulation of axons in the hippocampus with short high-frequency bursts that resemble in vivo activity patterns produces stable potentiation of postsynaptic responses when the bursts occur at intervals of 200 milliseconds but not 2 seconds. When a burst was applied to one input and a second burst applied to a different input to the same target neuron 200 milliseconds later, only the synapses activated by the second burst showed stable potentiation. This effect was observed even when the two inputs innervated completely different regions of the postsynaptic cells; but did not occur when the inputs were stimulated simultaneously or when the second burst was delayed by 2 seconds. Intracellular recordings indicated that the first burst extended the decay phase of excitatory postsynaptic potentials evoked 200 milliseconds later. These results suggest that a single burst of axonal stimulation produces a transient, spatially diffuse "priming" effect that prolongs responses to subsequent bursts, and that these altered responses trigger spatially restricted synaptic modifications. The similarity of the temporal parameters of the priming effect and the theta rhythm that dominates the hippocampal electroencephalogram (EEG) during learning episodes suggests that this priming may be involved in behaviorally induced synaptic plasticity.
Peptides As Functional Surfactants (2008)

Peptides offer interesting alternatives to conventional surfactants in applications where renewability, biocompatibility, or added functionality may be desired. This review offers a brief overview of different classes of surface-active peptides and lipopeptides, covering molecules obtained from natural sources as well as those obtained by design. Bacterial lipopeptides are cyclic molecules containing a single fatty acyl moiety, which can exhibit ultralow interfacial tension as well as antimicrobial activities. Bacterial lipopeptides have been proposed for industrial applications such as bioremediation and oil recovery, but they suffer the dual disadvantages of being difficult to bioproduce at low cost and not being easily genetically engineered. A class of synthetic molecules related to bacterial lipopeptides are the peptide amphiphiles, in which a peptide headgroup is combined with a peptide or nonpeptide hydrophobic tail. Self-assembly of peptide amphiphiles has largely been studied in bulk solution rather than at interfaces, meaning that very little information is available on the interfacial properties of these designer molecules. A larger body of information is available for protein hydrolysates, products of the partial breakdown of low-cost proteins (usually food proteins) into a complex mixture of small peptides. Partial hydrolysis can improve the functional properties of many proteins, but the outcomes are difficult to predict or control, and useful functional properties may be associated with only a few minor components in the digest mix. Finally, designed peptide surfactants (Pepfactants), recently reported by the authors, are facially amphipathic molecules that self-assemble at fluid interfaces to give cohesive films stabilizing foams and emulsions. A change in the bulk solution conditions can switch off the interfacial film, leading to rapid foam or emulsion collapse. Pepfactants can be genetically engineered and bioproduced using standard methods, which represents an advantage over bacterial lipopeptides. While peptides have not been widely used in surfactant applications so far, recent developments may facilitate the incorporation of these interesting molecules into industrial and consumer products in the near future.
Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation (2002)

We have developed a novel real-time phase-resolved functional optical coherence tomography system that uses optical Hilbert transformation. When we use a resonant scanner in the reference arm of the interferometer, with an axial scanning speed of 4 kHz, the frame rate of both structural and Doppler blood-flow imaging with a size of 100 by 100 pixels is 10 Hz. The system has high sensitivity and a larger dynamic range for measuring the Doppler frequency shift that is due to moving red blood cells. Real-time images of in vivo blood flow in human skin obtained with this interferometer are presented.
La、Ce对球铁球化率、白口和缩松倾向的影响 (2006)

能否抑制碳化物形成与球化剂和孕育剂的成核性能相关。成核性能可以理解为由于加入变质剂而形成的核心数量以及核心的效力。球化剂和孕育剂的加入也会影响球铁凝固时的缩松倾向。一些球化剂和孕育剂能较好地防止缩松，而有些球化剂和孕育剂则促进缩松的形成。已经发现，使用不同的稀土元素对这种情况有明显影响。与使用含铈或含混合稀土的球化剂相比，使用加有纯镧的硅铁镁合金作为包内处理球化剂时，球铁的工艺性能得到令人惊奇的改善。成核性能明显改善，因而采用三明治法和盖包法进行球化处理时的白口和缩松倾向减小。
The Secreted Pyomelanin Pigment of Legionella pneumophila Confers Ferric Reductase Activity (2007)

The virulence of Legionella pneumophila is dependent upon its capacity to acquire iron. To identify genes involved in expression of its siderophore, we screened a mutagenized population of L. pneumophila for strains that were no longer able to rescue the growth of a ferrous transport mutant. However, an unusual mutant was obtained that displayed a strong inhibitory effect on the feoB mutant. Due to an insertion in hmgA that encodes homogentisate 1,2-dioxygenase, the mutant secreted increased levels of pyomelanin, the L. pneumophila pigment that is derived from secreted homogentisic acid (HGA). Thus, we hypothesized that L. pneumophila-secreted HGA-melanin has intrinsic ferric reductase activity, converting Fe(3+) to Fe(2+), but that hyperpigmentation results in excessive reduction of iron that can, in the case of the feoB mutant, be inhibitory to growth. In support of this hypothesis, we demonstrated, for the first time, that wild-type L. pneumophila secretes ferric reductase activity. Moreover, whereas the hyperpigmented mutant had increased secreted activity, an lly mutant specifically impaired for pigment production lacked the activity. Compatible with the nature of HGA-melanins, the secreted ferric reductase activity was positively influenced by the amount of tyrosine in the growth medium, resistant to protease, acid precipitable, and heterogeneous in size. Together, these data represent the first demonstration of pyomelanin-mediated ferric reduction by a pathogenic bacterium.
A New Member of the Escherichia coli fad Regulon: Transcriptional Regulation of fadM ( ybaW ) (2009)

Recently, Nie and coworkers (L. Nie, Y. Ren, A. Janakiraman, S. Smith, and H. Schulz, Biochemistry 47:9618-9626, 2008) reported a new Escherichia coli thioesterase encoded by the ybaW gene that cleaves the thioester bonds of inhibitory acyl-coenzyme A (CoA) by-products generated during beta-oxidation of certain unsaturated fatty acids. These authors suggested that ybaW expression might be regulated by FadR, the repressor of the fad (fatty acid degradation) regulon. We report mapping of the ybaW promoter and show that ybaW transcription responded to FadR in vivo. Moreover, purified FadR bound to a DNA sequence similar to the canonical FadR binding site located upstream of the ybaW coding sequence and was released from the promoter upon the addition of long-chain acyl-CoA thioesters. We therefore propose the designation fadM in place of ybaW. Although FadR regulation of fadM expression had the pattern typical of fad regulon genes, its modulation by the cyclic AMP (cAMP) receptor protein-cAMP complex (CRP-cAMP) global regulator was the opposite of that normally observed. CRP-cAMP generally acts as an activator of fad gene expression, consistent with the low status of fatty acids as carbon sources. However, glucose growth stimulated fadM expression relative to acetate growth, as did inactivation of CRP-cAMP, indicating that the complex acts as a negative regulator of this gene. The stimulation of fadM expression seen upon deletion of the gene encoding adenylate cyclase (Deltacya) was reversed by supplementation of the growth medium with cAMP. Nie and coworkers also reported that growth on a conjugated linoleic acid isomer yields much higher levels of FadM thioesterase activity than does growth on oleic acid. In contrast, we found that the conjugated linoleic acid isomer was only a weak inducer of fadM expression. Although the gene is not essential for growth, the high basal level of fadM expression under diverse growth conditions suggests that the encoded thioesterase has functions in addition to beta-oxidation.
Epithelial-to-Pericyte Transition in Cancer (2017)

During epithelial-to-mesenchymal transition (EMT), cells lose epithelial characteristics and acquire mesenchymal properties. These two processes are genetically separable and governed by distinct transcriptional programs, rendering the EMT outputs highly heterogeneous. Our recent study shows that the mesenchymal products generated by EMT often express multiple pericyte markers, associate with and stabilize blood vessels to fuel tumor growth, thus phenotypically and functionally resembling pericytes. Therefore, some EMT events represent epithelial-to-pericyte transition (EPT). The serum response factor (SRF) plays key roles in both EMT and differentiation of pericytes, and may inherently confer the pericyte attributes on EMT cancer cells. By impacting their intratumoral location and cell surface receptor expression, EPT may enable cancer cells to receive and respond to angiocrine factors produced by the vascular niche, and develop therapy resistance.
Green Tea Protection Against Age-Dependent Ethanol-Induced Oxidative Stress (2004)

Ethanol intoxication leads to oxidative stress, which may be additionally enhanced by aging. The aim of this study was to investigate the influence of green tea as a source of water-soluble antioxidants on the ability to prevent oxidative stress in aged rats sub-chronically intoxicated with ethanol. Two-, 12-, and 24-mo-old male Wistar rats were divided into 4 experimental groups: (1) control, (2) green tea, (3) ethanol, and (4) ethanol and green tea. Ethanol intoxication produced age-dependent decrease in the activity of serum superoxide dismutase, glutathione peroxidase, and reductase and in levels of glutathione (GSH), vitamins C, E, and A, and beta-carotene. Changes in the serum antioxidative ability were accompanied by enhanced oxidative modification of lipid (increase in lipid hydroperoxides, malondiadehyde, and 4-hydroxynonenal levels) and protein (rise in carbonyl group levels). Green tea partially protected against changes in antioxidant enzymatic as well as nonenzymatic parameters produced by ethanol and enhanced by aging. Administration of green tea significantly protects cellular components such as lipids and proteins against oxidative modification. Results indicate that green tea effectively protects blood serum against oxidative stress produced by ethanol as well as aging.
Integration aspects of Flowfill and spin-on-glass process for sub-0.35 μm interconnects (2002)

Flowfill is a silane based process that offers local planarization similar to SOG. For sub-0.35 /spl mu/m devices, both processes are required for global planarization for critical metal layers. Integration aspects are studied for Flowfill and SOG, both in combination with CMP. The polishing process does not change the chemical stability or dielectric constant of these processes. There is an influence on the CMP nonuniformity and via resistance when Flowfill is annealed in forming gas. In case of SOG, the O/sub 2/ stripping process modifies the SOG layer, which leads to a higher dielectric constant. Flowfill has a positive influence on electromigration stress in Al lines, but leads to reduced device lifetimes in comparison to the SOG/CMP process.
Research Progress of Twinning Induced Plasticity(TWIP) Steel (2010)

This paper summarized the development of twinning induced plasticity(TWIP) steel.It emphatically introduced that a key factor for TWIP effect is to have a stable austenitic texture and a low stacking fault energy,effect of major alloying components on structure and properties of TWIP steel,the stacking fault energy and its calculation,mechanical properties of the first and second generations of TWIP steel,impact of temperature,strain rate and processing technique on TWIP steel,micro-mechanism of TWIP effect.
Quantitative reasoning and problem-solving strategy of children in different ethnic groups (2009)

The purpose of the research is to explore second graders' concept of number development and quantitative reasoning. For this purpose, there were two stages of trials for the children. The first trial was concrete objects. After three months, the children participated in the second trial of half concrete objects. Since understanding the process of solving problems in children is necessary, the researcher observed how children used strategies to discriminate numerosity. After that, the researcher interviewed some students. The research sample came from second graders in Taiwan and Hawaii elementary schools. Twenty students participated in each place. In addition, it was observed whether numerical discrimination strategy in children is the same as in adults. The sample included 20 adults. The result of the research showed that Taiwanese and Hawaiian children reached up to a 90% rate of correct answers in the trial of real objects. However, in the trial of half concrete objects, the rate of correct answers was down to 65%, especially when children compared two quantities of 5:6 ratios. As for strategy, the strategies of the children who gave correct answers were the same as the adults who gave completely correct answers. Because their discrimination was not influenced by distance effect, they could judge the numbers correctly. However, the children who got 90% correct answers were impacted by distance effect, but not influenced by the size of the object. The research suggests that teachers give children real objects to estimate or discriminate the quantities of numbers in their lives.
Environment generation for validating event-driven software using model checking (2010)

Event-driven systems maintain an ongoing dialog with their environment. Examples include: distributed programs, where each process reacts to received messages by performing computation and sending messages to peers; graphical user interfaces (GUI), where the interface software and the underlying application react to user inputs and web-based applications, where presentation, business logic and storage tier functionality react to user inputs and response from other web-based services. Such applications are difficult to test because the set of possible interaction sequences between the system and its environment can be very large and governed by complex constraints. The exhaustive nature of software model checking techniques offers hope for effectively validating such systems, however, they only work for closed systems. Previously, the authors developed the Bandera Environment Generator, which given an open system, called a unit under analysis, closes it with a model of its environment. The authors&apos; previous work on environment generation has focused on developing broadly applicable mechanisms for modelling environment behaviour. The generality of this approach often makes it difficult to produce environment models that enable precise and efficient system analysis. The authors&apos; experience shows that by exploiting information about the application domain, generated environments can be made both more precise and more efficient for model checking. This study presents the concept of domain-specific environment generation, details techniques for customising environment generation for the domain of event-driven software, and assesses those techniques on the domains of GUI and web-based applications.
Stable and efficient electrophosphorescent organic light-emitting devices grown by organic vapor phase deposition (2005)

An electrophosphorescent organic light-emitting device (PHOLED™) employing fac-tris(2-phenylpyridine)iridium [Ir(ppy)3] as the green emitting phosphor has been fabricated using a pilot-production organic vapor phase deposition (OVPD™) system. Highly controlled mass transport of the organic vapor to the substrate results in deposition rates of over 10Å∕s and spatial uniformity better than ±2% across a 150mm×150mm substrate with less than ±2% run-to-run variations. The device current–voltage, luminous efficiency, and operational lifetime performances are compared to those of a similar device grown by conventional vacuum thermal evaporation (VTE). The green OVPD-grown PHOLED exhibits a maximum external quantum efficiency of (7.0±0.1)% at a luminance of 1000cd∕m2, comparable to the VTE device performance. The operational lifetime of the OVPD-grown devices was found to be comparable to or even somewhat longer than the lifetime achieved by VTE. Furthermore, PHOLEDs with emissive layers deposited at 4.8 and 10.8Å∕s are compared, and demonstrate equivalent performance.
Long-term enzyme replacement therapy is associated with reduced proteinuria and preserved proximal tubular function in women with Fabry disease (2013)

The data indicate that long-term ERT is associated with a reduction in albuminuria and glomerular and tubular urinary protein markers in women with Fabry disease and mild renal manifestations.
Collaborative augmented reality (2002)

Blending reality and virtuality, these interfaces let users see each other, along with virtual objects, allowing communication behaviors much more like face-to-face than like screen-based collaboration.
Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody specific to HIV-1 - gp41 (1990)

Journal Article Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody specific to HIV-1 - gp41 Get access Martin Felgenhauer, Martin Felgenhauer Institut für Angewandte Mikrobiologie, Universität für BodenkulturPeter Jordanstraße 82, A-1190 Wien, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar Johann Kohl, Johann Kohl Institut für Angewandte Mikrobiologie, Universität für BodenkulturPeter Jordanstraße 82, A-1190 Wien, Austria Search for other works by this author on: Oxford Academic PubMed Google Scholar Florian Rüker Florian Rüker * Institut für Angewandte Mikrobiologie, Universität für BodenkulturPeter Jordanstraße 82, A-1190 Wien, Austria *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 16, 25 August 1990, Page 4927, https://doi.org/10.1093/nar/18.16.4927 Published: 25 August 1990 Article history Accepted: 12 July 1990 Published: 25 August 1990
Expression, purification and charaterization of recombinant mouse MT5‐MMP protein products (1999)

We have recently identified the fifth member of the membrane-type matrix metalloproteinase subfamily, MT5-MMP/MMP24, which is expressed in a brain specific manner (Duanqing Pei (1999) J. Biol. Chem. 274, 8925-8932). To further characterize its enzymic properties, an expression construct was engineered to produce MT5-MMP as a soluble and active form by truncating its transmembrane domain. Stable expression cell lines were subsequently established from MDCK cells transfected with this construct. Unfortunately, purification of MT5-MMP from the culture media in large quantity proves to be difficult initially due to its rapid turnover via a mechanism which can be inhibited by a broad spectrum metalloproteinase inhibitor, BB94. Thus, BB94 was included in the cell culture medium and throughout the purification process except the final step of chromatography to protect MT5-MMP from destruction. Purified to homogeneity and free of the synthetic inhibitor, MT5-MMP can activate progelatinase A efficiently in a TIMP2 sensitive fashion. A preliminary screen for its potential substrates among extracellular matrix components identified the proteoglycans as the preferred substrates for MT5-MMP. Furthermore, it is determined that the stability of purified MT5-MMP is temperature dependent with rapid destruction at 37 degrees C, but being relatively stable at temperatures 4 degrees C or lower. These observations establish MT5-MMP as a proteoglycanase with a short half-life at body temperature, which may be critical for tightly controlled turnover of ECM components such as those in the brain.