gebhart/patrepeval huggingface dataset:
Signal transduction through decay-accelerating factor. Interaction of glycosyl-phosphatidylinositol anchor and protein tyrosine kinases p56lck and p59fyn 1 (1992)

Abstract Decay-accelerating factor (DAF or CD55) is a 70-kDa glycosyl-phosphatidylinositol (GPI)-anchored protein that protects cells from complement-mediated lysis by either preventing the formation of or dissociating C3 convertases. Cross-linking of DAF on human peripheral T cells by polyclonal antibodies has previously been reported to lead to lymphocyte proliferation. Two mAb, both mapping to the third short consensus repeat region of DAF, were able to trigger proliferation of human peripheral T cells. To determine the role of the GPI anchor in cell activation, we transfected EL-4 murine thymoma cells with cDNA encoding either DAF or a transmembrane form of DAF (DAF-TM). The DAF-transfected cells were able to transduce late activation events as evidenced by IL-2 production, whereas DAF-TM transfected cells were unable to do so. The GPI-anchored DAF was able to transduce early activation events leading to the tyrosine phosphorylation of a 40-kDa protein and several proteins in the 85-95 kDa range--an event absent in DAF-TM-transfected cells. Furthermore, anti-DAF immunoprecipitates of DAF-transfected cells contain tyrosine kinase activity leading to the phosphorylation of 40-, 56-60-, and 85-kDa proteins, whereas anti-DAF immunoprecipitates of DAF-TM-transfected cells did not have an associated kinase activity. Both p56lck and p59fyn were associated with DAF in DAF-transfected EL-4 cells. In HeLa cells transfected with fyn, DAF associated with p59fyn. This complex of DAF with src family protein tyrosine kinases requires the GPI anchor and suggests a pathway for signaling through GPI-anchored membrane proteins.
Distortion of quantitative genomic and expression hybridization by Cot-1 DNA: mitigation of this effect (2005)

Cross-hybridization of repetitive sequences in genomic and expression arrays is reported to be suppressed with repeat-blocking nucleic acids (C(o)t-1 DNA). Contrary to expectation, we demonstrated that C(o)t-1 also enhanced non-specific hybridization between probes and genomic targets. When added to target DNA, C(o)t-1 enhanced hybridization (2.2- to 3-fold) to genomic probes containing conserved repetitive elements. In addition to repetitive sequences, C(o)t-1 was found to be enriched for linked single copy (sc) sequences. Adventitious association between these sequences and probes distort quantitative measurements of the probes hybridized to desired genomic targets. Quantitative microarray hybridization studies using C(o)t-1 DNA are also susceptible to these effects, especially for probes that map to genomic regions containing conserved repetitive sequences. Hybridization measurements with such probes are less reproducible in the presence of C(o)t-1 than for probes derived from sc regions or regions containing divergent repeat elements, a finding with significant ramifications for genomic and expression microarray studies. We mitigated the requirement for C(o)t-1 either by hybridizing with computationally defined sc probes lacking repeats or by substituting synthetic repetitive elements complementary to sequences in genomic probes.
Functionalization of Carbon Nanotubes via Dissolving Metal Reductions (2007)

In this study we present direct sidewall functionalization of single-walled nanotubes, carried out via two different dissolving metal reduction methods. A modified Birch reduction scheme and a novel synthetic route were applied to form carbanion complexes on the surface of the nanotubes in liquid NH3 and tetrahydrofuran, respectively. The carbanion complex was reacted with a series of alkyl and aryl halogenides resulting in the corresponding SWNT derivatives. We determined the chemical composition and the thermal stability of the functionalized nanotubes by thermogravimetry/mass spectrometry and pyrolysis-gas chromatography. Characteristic decomposition peaks, observed in the temperature range of 350-600 degrees C, suggest the formation of covalent derivatives upon functionalization that is also supported by Raman spectroscopic studies. The influence of the functional groups and the type of nanotubes on the thermal stability, as well as the relationship between the functional group content and the intensity of the Raman D peak are discussed.
A high performance 180 nm generation logic technology (2002)

A 180 nm generation logic technology has been developed with high performance 140 nm L/sub GATE/ transistors, six layers of aluminum interconnects and low-/spl epsi/ SiOF dielectrics. The transistors are optimized for a reduced 1.3-1.5 V operation to provide high performance and low power. The interconnects feature high aspect ratio metal lines for low resistance and fluorine doped SiO/sub 2/ inter-level dielectrics for reduced capacitance. 16 Mbit SRAMs with a 5.59 /spl mu/m/sup 2/ 6-T cell size have been built on this technology as a yield and reliability test vehicle.
Case 14-2008 (2008)

A 78-year-old man was admitted to the inpatient psychiatry service because of anergia and anhedonia of 33 months' duration. His symptoms began shortly after he was told he needed repair of an abdominal aortic aneurysm, persisted despite multiple trials of antidepressant medications, and worsened after repair of the aneurysm. An episode of major depression had occurred at the age of 58 years after coronary-artery bypass surgery and had responded to electroconvulsive therapy. A management decision was made.
Multiscale entropy analysis of biological signals (2005)

Traditional approaches to measuring the complexity of biological signals fail to account for the multiple time scales inherent in such time series. These algorithms have yielded contradictory findings when applied to real-world datasets obtained in health and disease states. We describe in detail the basis and implementation of the multiscale entropy (MSE) method. We extend and elaborate previous findings showing its applicability to the fluctuations of the human heartbeat under physiologic and pathologic conditions. The method consistently indicates a loss of complexity with aging, with an erratic cardiac arrhythmia (atrial fibrillation), and with a life-threatening syndrome (congestive heart failure). Further, these different conditions have distinct MSE curve profiles, suggesting diagnostic uses. The results support a general "complexity-loss" theory of aging and disease. We also apply the method to the analysis of coding and noncoding DNA sequences and find that the latter have higher multiscale entropy, consistent with the emerging view that so-called "junk DNA" sequences contain important biological information.
THE INHIBITORY EFFECT OF THE MUSCARINIC AGONIST PILOCARPINE ON LYMPHOCYTE ACTIVATION INVOLVES IN SUPPRESSOR CELL FUNCTION THE IL-2 PATHWAY AND THE INCREASE (1992)

M) was not reversed by addition of exogenous recombinant Interleukin-2 (100-1000 IU/ml). Pilo- carpine did not inhibit Interleukin-2 (IL-2) production by human peripheral blood mononuclear cells but decreased the number of interleukin-2 receptor bearing cells (TAC positive cells). Furthermore, pilo- carpine increased the
Optimizing Greenhouse Corn Production: Materials and Methods (2010)

This document is one entry in a series of questions and answers originally posted to the Purdue University Department of Horticulture & Landscape Architecture’s Plant Growth Facility Web site (http://www.hort.purdue.edu/hort/facilities/greenhouse/CornMethod.shtml) regarding best practices for corn plant growth in a greenhouse. Controlled studies were conducted with the goals of optimizing growth and yield; developing standardized methodology using readily available commercial materials; and providing recommendations for both high-tech and low-tech facilities. Digital images showing treatment differences are included, as well as statistical analysis of data. A summary of recommendations; materials and methods description; and a reference list are available in separate documents.
Complement plays an essential role in shock following intestinal ischaemia in rats (1996)

Intestinal ischaemia lasting more than 30 min in rats causes fatal systemic shock. Systemic shock was suppressed by preadministration of cobra venom factor (CVF), which reduced the serum complement to less than 5% of the normal level, indicating that complement is involved in the syndrome. After complement activation, anaphylatoxins such as C3a and C5a are generated, and their activity is restricted by carboxypeptidases which remove C-terminal arginine from such bioactive peptides. As expected, preadministration of a carboxypeptidase inhibitor enhanced the systemic shock induced by the intestinal ischaemia. However, when the complement level was suppressed by CVF treatment, no fatal systemic shock was induced by the intestinal ischaemia even with preadministration of the carboxypeptidase inhibitor. These results indicate that complement plays a crucial role in systemic shock induced by intestinal ischaemia, and that anaphylatoxins generated by the complement activation should be involved in induction of the shock syndrome.
Towards Multi-tenant Performance SLOs (2012)

As traditional and mission-critical relational database workloads migrate to the cloud in the form of Database-as-a-Service (DaaS), there is an increasing motivation to provide performance goals in Service Level Objectives (SLOs). Providing such performance goals is challenging for DaaS providers as they must balance the performance that they can deliver to tenants and the data center's operating costs. In general, aggressively aggregating tenants on each server reduces the operating costs but degrades performance for the tenants, and vice versa. In this paper, we present a framework that takes as input the tenant workloads, their performance SLOs, and the server hardware that is available to the DaaS provider, and outputs a cost-effective recipe that specifies how much hardware to provision and how to schedule the tenants on each hardware resource. We evaluate our method and show that it produces effective solutions that can reduce the costs for the DaaS provider while meeting performance goals.
Angiotensin II AT1 Receptor Antagonists. Clinical Implications of Active Metabolites (2003)

ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTAngiotensin II AT1 Receptor Antagonists. Clinical Implications of Active MetabolitesBoris Schmidt and Bernhard SchiefferView Author Information Clemens Schöpf Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Petersenstrasse 22, D-64287 Darmstadt, Germany, and Department of Cardiology and Angiology, Medizinische Hochschule Hannover, Hannover, Germany Cite this: J. Med. Chem. 2003, 46, 12, 2261–2270Publication Date (Web):May 8, 2003Publication History Received25 September 2002Published online8 May 2003Published inissue 1 June 2003https://pubs.acs.org/doi/10.1021/jm0204237https://doi.org/10.1021/jm0204237review-articleACS PublicationsCopyright © 2003 American Chemical SocietyRequest reuse permissionsArticle Views1807Altmetric-Citations101LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anatomy,Antagonists,Metabolism,Peptides and proteins,Receptors Get e-Alerts
Neurological disorders in Gulf War veterans (2006)

We present a review of neurological function in Gulf War veterans (GWV). Twenty-two studies were reviewed, including large hospitalization and registry studies, large population-based epidemiological studies, investigations of a single military unit, small uncontrolled studies of ill veterans and small controlled studies of veterans. In nearly all studies, neurological function was normal in most GWVs, except for a small proportion who were diagnosed with compression neuropathies (carpal tunnel syndrome or ulnar neuropathy). In the great majority of controlled studies, there were no differences in the rates of neurological abnormalities in GWVs and controls. In a national US study, the incidence of amyotrophic lateral sclerosis (ALS) seems to be significantly increased in GWVs, compared to the rate in controls. However, it is possible that military service, in general, might be associated with an increased risk of ALS, rather than Gulf War service in particular. Taken together, the conclusion is that if a neurological examination in a GWV is within normal limits, then extensive neurological testing is unlikely to diagnose occult neurological disorders.
5‐Lipoxygenase‐activating protein stimulates the utilization of arachidonic acid by 5‐lipoxygenase (1993)

5-Lipoxygenase (5-LO) and its activating protein (FLAP) are both required for cellular leukotriene (LT) synthesis, with 5-LO catalyzing both the synthesis of (5S)-5-hydroperoxy-6,8,11,14-eicosatetraenoic acid (5-HPETE) from arachidonic acid and the subsequent synthesis of LTA4 from 5-HPETE. We have previously expressed both human 5-LO and human FLAP to high levels in Spodoptera frugiperda (Sf9) insect cells, using recombinant baculoviruses. To study the mechanism by which FLAP activates 5-LO, we compared cellular 5-LO activity in Sf9 cells expressing this enzyme to that in Sf9 cells coexpressing FLAP and 5-LO. In this system, FLAP stimulates the utilization of arachidonic acid by 5-LO as a substrate, and increases the efficiency with which 5-LO converts 5-HPETE to LTA4. LT synthesis in cells coexpressing FLAP and 5-LO is inhibited by 3-[1-(p-chlorophenyl)-5-isopropyl-3-tert-butylthio-1H-indol-2-yl]-2,2- dimethyl-propanoic acid (MK-886), an LT biosynthesis inhibitor which specifically binds to FLAP. These studies in Sf9 cells, together with our recent demonstration that FLAP specifically binds arachidonic acid, suggests that FLAP activates 5-LO by acting as an arachidonic acid transfer protein.
The Outcome of Ex Vivo TIL Expansion Is Highly Influenced by Spatial Heterogeneity of the Tumor T-Cell Repertoire and Differences in Intrinsic In Vitro Growth Capacity between T-Cell Clones (2020)

Culture-induced changes in clonal composition are likely to affect tumor reactivity of TIL preparations. TCR deep sequencing provides important insights into the factors that govern the outcome of <i>in vitro</i> TIL expansion and thereby a path toward optimization of the production of TIL preparations with high therapeutic efficacy.<i>See related commentary by Lozano-Rabella and Gros, p. 4177</i>.
[Possibilities for the deep bacterial destruction of 2,4,6-trinitrotoluene]. (1988)

The possibility of 2,4,6-trinitrotoluene (TNT) deep destruction (the aromatic cycle fission inclusive) by Pseudomonas fluorescens B-3468 is reported for the first time. The formation of nitrogen-free metabolites, viz. phloroglucinol and pyrogallol, is preceded by the NAD(P)H-dependent deamination of 2,4-diamino-6-nitrotoluene (2,4-DA), a TNT intermediate. 30% of 2,4-DA nitrogen in released as ammonium under the action of an induced cell-free extract in the presence of the preferential co-factor NADH (together with FAD). The elevated pyrogallol-decomposing activity in cells grown on 2,4-DA, phloroglucinol and pyrogallol as well as the induction of the pyrocatechase activity in cells grown on the above substrates, together with the earlier reported accumulation of phloroglucinol and pyrogallol upon 2,4-DA utilization, indicated that the enzyme might be involved in the TNT cycle cleavage. The participation of NADH-dependent glutamate dehydrogenase in 2,4-DA nitrogen utilization is supported by experimental evidence.
Identification of proteins that may directly interact with human RPA (2010)

RPA, which consisted of three subunits (RPA1, 2 and 3), plays essential roles in DNA transactions. At the DNA replication forks, RPA binds to single-stranded DNA region to stabilize the structure and to assemble other replication proteins. Interactions between RPA and several replication proteins have been reported but the analysis is not comprehensive. We systematically performed the qualitative analysis to identify RPA interaction partners to understand the protein-protein interaction at the replication forks. We expressed in insect cells the three subunits of human RPA, together with one replication protein, which is present at the forks under normal conditions and/or under the replication stress conditions, to examine the interaction. Among 30 proteins examined in total, it was found that at least 14 proteins interacted with RPA. RPA interacted with MCM3-7, MCM-BP and CDC45 proteins among the proteins that play roles in the initiation and the elongation of the DNA replication. RPA bound with TIPIN, CLASPIN and RAD17, which are involved in the DNA replication checkpoint functions. RPA also bound with cyclin-dependent kinases and an amino-terminal fragment of Rb protein that negatively regulates DNA replication. These results suggest that RPA interacts with the specific proteins among those that play roles in the regulation of the replication fork progression.
Pristine Simple Oxides as Visible Light Driven Photocatalysts: Highly Efficient Decomposition of Organic Compounds over Platinum-Loaded Tungsten Oxide (2008)

Tungsten oxide loaded with nanoparticulate platinum is demonstrated to exhibit high activity for the decomposition of organic compounds both in liquid and gas phases; the activity was almost comparable to that of TiO2 under UV light irradiation and much higher than that of nitrogen-doped TiO2 under visible irradiation.
Recursive filters for optical flow (1995)

Working toward efficient (real-time) implementations of optical flow methods, the authors have applied simple recursive filters to achieve temporal smoothing and differentiation of image intensity, and to compute 2d flow from component velocity constraints using spatiotemporal least-squares minimization. Accuracy in simulation is similar to that obtained in the study by Barren et al. (1994), while requiring much less storage, less computation, and shorter delays.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Cell-cell communication regulates the effects of protein aspartate phosphatases on the phosphorelay controlling development in Bacillus subtilis. (1996)

Rap phosphatases are a recently discovered family of protein aspartate phosphatases that dephosphorylate the Spo0F--P intermediate of the phosphorelay, thus preventing sporulation of Bacillus subtilis. They are regulators induced by physiological processes that are antithetical to sporulation. The RapA phosphatase is induced by the ComP-ComA two-component signal transduction system responsible for initiating competence. RapA phosphatase activity was found to be controlled by a small protein, PhrA, encoded on the same transcript as RapA. PhrA resembles secreted proteins and the evidence suggests that it is cleaved by signal peptidase I and a 19-residue C-terminal domain is secreted from the cell. The sporulation deficiency caused by the uncontrolled RapA activity of a phrA mutant can be complemented by synthetic peptides comprising the last six or more of the C-terminal residues of PhrA. Whether the peptide controls RapA activity directly or by regulating its synthesis remains to be determined. Complementation of the phrA mutant can also be obtained in mixed cultures with a wild-type strain, suggesting the peptide may serve as a means of communication between cells. Importation of the secreted peptide required the oligopeptide transport system. The sporulation deficiency of oligopeptide transport mutants can be suppressed by mutating the rapA and rapB genes or by introduction of a spo0F mutation Y13S that renders the protein insensitive to Rap phosphatases. The data indicate that the sporulation deficiency of oligopeptide transport mutants is due to their inability to import the peptides controlling Rap phosphatases.
Reversibility of the duocarmycin A and SA DNA alkylation reaction (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReversibility of the duocarmycin A and SA DNA alkylation reactionDale L. Boger and Weiya YunCite this: J. Am. Chem. Soc. 1993, 115, 21, 9872–9873Publication Date (Print):October 1, 1993Publication History Published online1 May 2002Published inissue 1 October 1993https://pubs.acs.org/doi/10.1021/ja00074a093https://doi.org/10.1021/ja00074a093research-articleACS PublicationsRequest reuse permissionsArticle Views256Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Impact of phase and amplitude errors on array performance (2009)

This paper analyzes the effect of errors in antenna weights on the performance of adaptive array systems, both in the case when an array is used to maximize the gain in a desired direction and in the case when an array is used to null interfering signals. We begin by deriving an explicit characterization of the loss in array gain due to phase errors in the optimal antenna weights. Then, we examine interference rejection in the presence of amplitude and phase errors in the antenna weights. We prove that the loss in interference rejection is independent of the number of antennas. For both cases, we give numerical simulations that validate our analysis.