gebhart/patrepeval huggingface dataset:
Methods for the calculation of surface free energy of solids (2007)

Purpose: The main purpose of this paper is the analysis of the most common methods for the calculation of the surface free energy (SFE) of solids, utilising the results of the contact angle measurements. The calculation deals also with the SFE at the interface, especially that at the surface of polymers and polymeric materials. The survey has been meant to ease the understanding of physical processes occurring at the solid-liquid interface and to help to find proper measuring methods with respect to various physical systems. Design/methodology/approach: The presented analysis has been based on the papers of the fundamental nature as well as on the specialised literature reports. The results of the experimental and theoretical studies of the author of this article are also considered. Findings: Different assumptions have been made in the individual methods for calculating the SFE of polymeric materials. Thus, the SFE values for a given material, obtained by various methods and with use of different measuring liquids, are not consistent. The method for the calculation of the SFE of porous or granulated materials, powders, and fibres, in which the Washburn equation is utilised, is very useful in practice. Currently, there is no appropriate alternative to this method. Research limitations/implications: The method for calculating the SFE with use of the equation of state requires further investigation, both experimental and theoretical. Further studies on the phenomena associated with penetration of the measuring liquids into the bulk of an examined material and on the relevant changes concerning this material, including the changes in its SFE, have also to be carried out. Practical implications: The presented results of the investigations may be applied in optimisation of the current and derivation of the new methods for calculating the SFE of solids and liquids, including mainly the SFE of polymers in the solid state. Originality/value: Because of differences in the assumptions made in most of the methods for the SFE calculation and of differences in the interactions between the individual measuring liquids and the examined material, the SFE values for various polymer materials may be compared with one another only when the contact angle measurements have been made using the same measuring liquids and when the SFE calculations have been performed by the same method.
Individualization of Single‐Walled Carbon Nanotubes: Is the Solvent Important? (2005)

The influence of different solvents on the individualization of single-walled carbon nanotubes (SWNTs) was investigated by several methods. A stable solution of individual SWNTs in o-dichlorobenzene was obtained by a combination of ultrasonication and ultracentrifugation. Atomic force microscopy height analysis confirmed that the final supernatant solution contained 85 % of individual tubes with a diameter equal to or less than 1.5 nm, which is consistent with the average diameter of arc-discharge tubes. Both the solvent and the ultracentrifugation speed are important parameters in determining the final degree of individualized SWNTs. Notably, the SWNTs were stable in o-dichlorobenzene for more than eight months. This technique for obtaining highly individualized SWNTs in an organic solvent is expected to have great potential in many applications, such as functional materials and nanoelectronics.
Production of Eicosanoids and Other Oxylipins by Pathogenic Eukaryotic Microbes (2003)

Oxylipins are oxygenated metabolites of fatty acids. Eicosanoids are a subset of oxylipins and include the prostaglandins and leukotrienes, which are potent regulators of host immune responses. Host cells are one source of eicosanoids and oxylipins during infection; however, another potential source of eicosanoids is the pathogen itself. A broad range of pathogenic fungi, protozoa, and helminths produce eicosanoids and other oxylipins by novel synthesis pathways. Why do these organisms produce oxylipins? Accumulating data suggest that phase change and differentiation in these organisms are controlled by oxylipins, including prostaglandins and lipoxygenase products. The precise role of pathogen-derived eicosanoids in pathogenesis remains to be determined, but the potential link between pathogen eicosanoids and the development of TH2 responses in the host is intriguing. Mammalian prostaglandins and leukotrienes have been studied extensively, and these molecules can modulate Th1 versus Th2 immune responses, chemokine production, phagocytosis, lymphocyte proliferation, and leukocyte chemotaxis. Thus, eicosanoids and oxylipins (host or microbe) may be mediators of a direct host-pathogen "cross-talk" that promotes chronic infection and hypersensitivity disease, common features of infection by eukaryotic pathogens.
Fuzzy Backing Control of Truck and Two Trailers (2006)

For a number of years, truck backer-upper problem has served as a benchmark for control among the practitioners of computational intelligence. In our works from the past we have shown how decomposition of the control problem and subsequent segmentation of the control system leads to substantial control performance improvement. In current paper, this control principle is extended to the much more complex two-trailer backing problem with great success.
High density interconnect verification using voltage contrast electron beam (2002)

High reliability testing of multi-chip module (MCM) substrates has been demonstrated using voltage contrast electron beam (VCEB) open-shorts testing. Features as small as 100 mu m are probed over an area of 10 cm*10 cm in a tool developed by installing high-precision digital electronics and a novel large field of view secondary electron analyzer into a conventional scanning electron microscope. These modifications in conjunction with sophisticated calibration and data analysis techniques have produced the research tool which is now routinely used to test substrates fabricated at Microelectronics and Computer Technology Corporation (MCC) as well as many fabricated by MCC's shareholders. The performance of the system was assessed by testing MCM product substrates and special test substrates. Various combinations of conductors and insulators were tested. The test targets were designed with numerous feature sizes over the entire field of view so that probing accuracy and test uniformity could be characterized.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Evaluation and Therapy of Disturbed Motor Control in Spastic Paresis (1997)

This article, based on an oral presentation, reviews selected aspects of the evaluation and therapy of hemiparetic locomotor disorders. The first part describes different types of disturbed locomotor control (impairments), determined by analysis of concomitant records of lower extremity movements and muscle activations during walking. These are: type I, hyperactive stretch reflexes (spasticity), type II, lack of adequate muscle activations (paresis), type III, excessive coactivation of antagonist muscles (loss of sequential activations) or type IV, a complex disorder. These selective impairments imply first, that in most subjects a component of the locomotor control disorder can be identified as the key factor and second, that such information should guide therapy. To complete the picture, changes in the mechanical properties of the muscle-tendon unit (non-neural component) may also contribute to the locomotor disorder. This classification of selective impairments challenges the traditional emphasis on spasticity as the dominant disorder in all subjects.
Expression of LINE-1 retrotransposons in human breast cancer (1994)

These results raise the possibility that L1Hs expression may contribute to the origin or progression of some breast cancers.
β4 Integrin Is a Transforming Molecule that Unleashes Met Tyrosine Kinase Tumorigenesis (2005)

Cell multiplication in the absence of integrin-derived adhesive signals (anchorage-independent growth) is the phenotypic hallmark of neoplastic transformation. Therefore, the frequently observed up-regulation of some integrins in tumors has been interpreted as an epiphenomenon and not as a causative factor of oncogenic conversion. beta4 integrin stimulates proliferation and survival of epithelial cells and is overexpressed in human carcinomas, often in concomitance with up-regulation of the Met tyrosine kinase receptor for hepatocyte growth factor. Met is not endowed with transforming ability but can exploit the beta4 cytoplasmic tail as a substrate/adaptor for amplification of mitogenic and antiapoptotic responses, independently of cell adhesion. Here, we show that overexpression of beta4 is sufficient to transform rodent fibroblasts, enhances anchorage-independent growth of breast carcinoma cells, and induces tumorigenesis in nude mice; conversely, RNA interference-mediated depletion abrogates the transformed phenotype of neoplastic cells. These autonomous oncogenic properties are dramatically exacerbated upon Met coexpression, suggesting that the integrin can instigate the latent tumorigenic potential of the kinase. A beta4 nonadhesive variant still cooperates with Met for cellular transformation, confirming the adhesion-independent function of beta4 in magnification of Met biological effects. Conversely, a beta4 signaling-incompetent mutant that cannot be efficiently tyrosine phosphorylated by Met and displays reduced ability to activate phosphatidylinositol 3-kinase-dependent and Ras-dependent pathways aborts transformation. Our findings define beta4 as a signaling accomplice (a "servo-oncogene") of tyrosine kinase proto-oncogenes in primary carcinogenesis, evoke an unorthodox function for a prototypic adhesion molecule in the positive regulation of anchorage-independent growth, and suggest the use of beta4 as a target for anticancer therapy.
From theory to practice: an overview of MIMO space-time coded wireless systems (2003)

This paper presents an overview of progress in the area of multiple input multiple output (MIMO) space-time coded wireless systems. After some background on the research leading to the discovery of the enormous potential of MIMO wireless links, we highlight the different classes of techniques and algorithms proposed which attempt to realize the various benefits of MIMO including spatial multiplexing and space-time coding schemes. These algorithms are often derived and analyzed under ideal independent fading conditions. We present the state of the art in channel modeling and measurements, leading to a better understanding of actual MIMO gains. Finally, the paper addresses current questions regarding the integration of MIMO links in practical wireless systems and standards.
Immunogenicity of polysaccharides conjugated to peptides containing T- and B-cell epitopes (1994)

To develop a general model of polysaccharide-peptide vaccine, we have investigated the efficiency of linear peptides derived from protein SR, and adhesin of the I/II protein antigen family of oral streptococci, to act as carriers for two T cell-independent polysaccharides: serogroup f polysaccharide from Streptococcus mutans OMZ 175 (poly f) and Saccharomyces cerevisiae mannan. Peptide 3 (YEKEPTPPTRTPDQ) and peptide 6 (TPEDPTDPTDPQDPSS), accessible on the native SR protein as demonstrated by their reactivity in enzyme-linked immunosorbent assays with rat antisera raised against protein SR, correspond to immunodominant regions of SR. Peptide 3 contains at least one B- and one T-cell epitope, as demonstrated by its ability to induce peptide- and SR-specific antibody responses without any carrier and to stimulate the proliferation of rat lymph node cells primed either with free peptide or native SR, whereas peptide 6 contains only B-cell epitope(s). Peptide 3 was then covalently coupled though reductive amination to either poly f or mannan, and peptide 6 was coupled to poly f. Subcutaneous immunizations of rats with poly f-peptide 3 or mannan-peptide 3 conjugates produced a systemic immunoglobulin M (IgM) and IgG antibody response, and the elicited antibodies reacted with free poly f or mannan, peptide 3, protein SR, and S. mutans or S. cerevisiae whole cells. Rats immunized with poly f-peptide 6 did not develop any antipeptide or anti-SR response. Furthermore, a booster immunization of animals with poly f-peptide 3 or mannan-peptide 3 conjugates induced high titers of anti-peptide 3, anti-poly f, and antimannan antibodies, which occurred quickly. The response is anamnestic for the peptide and the polysaccharides and is characterized by an Ig switch from IgM to IgG. The data presented here confirm that the presence of B- and T-cell epitopes is necessary to induce an anamnestic antipeptide response and that a peptide containing relevant B- and T-cell epitopes can act as a good carrier in improving an antipolysaccharide anamnestic immune response.
Microbend fiber-optic sensor (1987)

Intensity modulation induced by microbending in multimode fibers is considered as a transduction mechanism for detecting environmental changes such as pressure, temperature, acceleration, and magnetic and electric fields. A generic microbend sensor has been defined and studied, and its components, such as sensing fiber, light source, optical fiber leads, and detector, have been examined and optimized. Finally, the generic microbend sensor has been tested demonstrating good performance.
Nucleotide sequence of the thermostable direct hemolysin gene of Vibrio parahaemolyticus (1985)

The gene encoding the thermostable direct hemolysin of Vibrio parahaemolyticus was characterized. This gene (designated tdh) was subcloned into pBR322 in Escherichia coli, and the functional tdh gene was localized to a 1.3-kilobase HindIII fragment. This fragment was sequenced, and the structural gene was found to encode a mature protein of 165 amino acid residues. The mature protein sequence was preceded by a putative signal peptide sequence of 24 amino acids. A putative tdh promoter, determined by its similarity to concensus sequences, was not functional in E. coli. However, a promoter that was functional in E. coli was shown to exist further upstream by use of a promoter probe plasmid. A 5.7-kilobase SalI fragment containing the structural gene and both potential promoters was cloned into a broad-host-range plasmid and mobilized into a Kanagawa phenomenon-negative V. parahaemolyticus strain. In contrast to E. coli, where the hemolysin was detected only in cell lysates, introduction of the cloned gene into V. parahaemolyticus resulted in the production of extracellular hemolysin.
Compound CAR T-cells as a double-pronged approach for treating acute myeloid leukemia (2018)

Acute myeloid leukemia (AML) bears heterogeneous cells that can consequently offset killing by single-CAR-based therapy, which results in disease relapse. Leukemic stem cells (LSCs) associated with CD123 expression comprise a rare population that also plays an important role in disease progression and relapse. Here, we report on the robust anti-tumor activity of a compound CAR (cCAR) T-cell possessing discrete scFv domains targeting two different AML antigens, CD123, and CD33, simultaneously. We determined that the resulting cCAR T-cells possessed consistent, potent, and directed cytotoxicity against each target antigen population. Using four leukemia mouse models, we found superior in vivo survival after cCAR treatment. We also designed an alemtuzumab safety-switch that allowed for rapid cCAR therapy termination in vivo. These findings indicate that targeting both CD123 and CD33 on AML cells may be an effective strategy for eliminating both AML bulk disease and LSCs, and potentially prevent relapse due to antigen escape or LSC persistence.
Quantum key distribution over a 48 km optical fibre network (2000)

Abstract The secure distribution of the secret random bit sequences known as ‘key’ material, is an essential precursor to their use for the encryption and decryption of confidential communications. Quantum cryptography is a new technique for secure key distribution with single-photon transmissions: Heisenberg's uncertainty principle ensures that an adversary can neither successfully tap the key transmissions, nor evade detection (eavesdropping raises the key error rate above a threshold value). We have developed experimental quantum cryptography systems based on the transmission of non-orthogonal photon states to generate shared key material over multikilometre optical fibre paths and over line-of-sight links. In both cases, key material is built up using the transmission of a single-photon per bit of an initial secret random sequence. A quantum-mechanically random subset of this sequence is identified, becoming the key material after a data reconciliation stage with the sender. Here we report the most recent results of our optical fibre experiment in which we have performed quantum key distribution over a 48 km optical fibre network at Los Alamos using photon interference states with the B92 and BB84 quantum key distribution protocols.
Interleukin-1 Receptor and Receptor Antagonist Gene Expression After Focal Stroke in Rats (1997)

Differential expression of IL-1 beta, IL-1ra, IL-1RI, and IL-1RII mRNAs after focal stroke may suggest a distinct role(s) for each component of the IL-1 system in ischemic injury. The data also stress the importance of evaluating all the components of a given cytokine system (eg, agonist, receptors, and natural antagonist) after focal stroke.
Purification and structural characterization of de-N-acetylated form of GD3 ganglioside present in human melanoma tumors (2007)

The presence of gangliosides containing de-N-acetylated sialic acids in human tissues has been so far shown by using mouse monoclonal antibodies specific for the de-N-acetylated forms, but the isolation and chemical characterization of such compounds have not yet been performed. Since indirect evidence suggested that de-N-acetylGD3 ganglioside could be present in human melanoma tumors, we analyzed the gangliosides purified from a 500-g pool of those tumors. The de-N-acetylGD3 that was found to migrate just below GD2 in thin-layer chromatography was isolated from the disialogangliosides by high-pressure liquid chromatography using the specific antibody SGR37 to monitor the elution. The amount of antigen was found to be 320 ng per gram of fresh tumor or 0.1% of total gangliosides. Gas chromatography-mass spectrometry analysis of the antibody-positive ganglioside showed that sialic acids were formed of one molecule of N-acetylneuraminic acid and one molecule of neuraminic acid. Radioactive re-N-acetylation of the antigen yielded a GD3-like ganglioside with the radioactive label on the external sialic acid. The constitutive fatty acids were found to differ markedly from those of GD3 and 9-O-acetylGD3 isolated from the same pool of tumors. The major fatty acids were C16:0 and C18:0 in de-N-acetylGD3, whereas GD3 and its 9-O-acetylated derivative contained a large amount of C24:1. These data show that de-N-acetylGD3 ganglioside is indeed present in human melanoma tumors, and the fatty acid content suggests the existence of a de-N-acetylase mostly active on the molecular species of gangliosides with short-chain fatty acids.
Analysis of the acute and chronic wound environments: the role of proteases and their inhibitors (1999)

To assess the differences in proteolytic activity of acute and chronic wound environments, wound fluids were collected from acute surgical wounds (22 samples) and chronic wounds (25 samples) of various etiologies, including mixed vessel disease ulcers, decubiti and diabetic foot ulcers. Matrix metalloproteinase (MMP) activity measured using the Azocoll assay was significantly elevated by 30 fold in chronic wounds (median 22.8 microg MMP Eq/ml) compared to acute wounds (median 0.76 microg MMP Eq/ml) (p < 0.001). The addition of the matrix metalloproteinase inhibitor Illomostat decreased the matrix metalloproteinase activity by approximately 90% in all samples, confirming that the majority of the activity measured was due to matrix metalloproteinases. Gelatin zymograms indicated predominantly elevated matrix metalloproteinase-9 with smaller elevations of matrix metalloproteinase-2. In addition tissue inhibitor of metalloproteinase-1 levels were analyzed in a small subset of acute and chronic wounds. When tissue inhibitor of metalloproteinase-1 levels were compared to protease levels there was an inverse correlation (p = 0.02, r = - 0.78). In vitro degradation of epidermal growth factor was measured by addition of 125I labelled epidermal growth factor to acute and chronic wound fluid samples. There was significantly higher degradation of epidermal growth factor in chronic wound fluid samples (mean 28.1%) compared to acute samples (mean 0.6%). This also correlated to the epidermal growth factor activity of these wound fluid samples (p < 0. 001, r = 0.64). Additionally, the levels of proteases were assayed in wound fluid collected from 15 venous leg ulcers during a nonhealing and healing phase using a unique model of chronic wound healing in humans. Patients with nonhealing venous leg ulcers were admitted to the hospital for bed rest and wound fluid samples were collected on admission (nonhealing phase) and after 2 weeks (healing phase) when the ulcers had begun to heal as evidenced by a reduction in size (median 12%). These data showed that the elevated levels of matrix metalloproteinase activity decreased significantly as healing occurs in chronic leg ulcers (p < 0.01). This parallels the processes observed in normally healing acute wounds. This data also supports the case for the addition of protease inhibitors in chronic wounds in conjunction with any treatments using growth factors.
Evaluation of a time-resolved fluorescence microscope using a phosphorescent Pt-porphine model system (1996)

A time-resolved fluorescence microscope is presented that allows the sensitive detection of delayed luminescent labels with decay times from one microsecond to several milliseconds. The microscope utilizes an argon ion laser chopped with an acoustooptical modulator as excitation light source in combination with a gated multichannel plate image intensifier in the image plane. A theoretical model for the detection efficiency of practically any time-resolved fluorescence microscope is verified using phosphorescent Pt-porphine-stained Sephadex beads. The detection efficiency of the presented setup was shown to be 42%, which is near the theoretical limit of 50% for non-saturated luminescent dyes. The suppression of prompt fluorescence signals was found to be 1:5,500. The Pt-porphine beads proved to be an excellent model system for time-resolved fluorescence microscopy, showing a high extinction coefficient and high phosphorescence quantum yield in aqueous environment under room temperature conditions. Furthermore, for the microscope described the decay time of the Pt-porphine beads of 47 microseconds is long enough to enable efficient suppression of the prompt fluorescence while maintaining a high excitation and emission duty cycle. This is considered to be of vital importance in order not to saturate the luminescence with the excitation intensities commonly used in fluorescence microscopy.
Investigation of drug absorption from the gastrointestinal tract of man. III. Metoprolol in the colon. (1985)

The colonic absorption of metoprolol was indirectly evaluated by measuring drug appearance in plasma following intravenous, jejunal or colonic infusion in six healthy volunteers. Plasma concentrations of alpha-hydroxymetoprolol and urinary excretion of the main metabolites were also measured. Plasma profiles of metoprolol after colonic and jejunal perfusion were similar, and the relative bioavailabilities of the drug from these two regions of the gut were not significantly different. The concentrations of alpha-hydroxymetoprolol, the major metabolite in plasma, were similar after jejunal and colonic perfusion, but higher than those observed after intravenous administration. The percentage of the dose recovered in urine over 24 h as two metabolites was not significantly influenced by the route of administration.