gebhart/patrepeval huggingface dataset:
Scopolamine-induced deficits in a two-trial object recognition task in mice (1997)

The purpose of the present study was to design an object recognition task in mice and characterize the effects of scopolamine in this paradigm. This task consisted of exposing mice for 6 or 10 min to an object in an open field (trial 1) and, after a delay (1-24 h), testing mice for 10 min with the object and a novel object (trial 2). Mice explored the novel object more than the familiar object as the inter-trial delay decreased and/or the duration of trial 1 increased. Administration of scopolamine (0.3, 1 and 3 mg kg-1, s.c.) before trial 1 reduced recognition performance on trial 2 after a 3 h inter-trial delay and induced other behavioural effects, including an increase in locomotor activity on trial 1. Methylscopolamine (1 mg kg-1) had no effect on recognition performance. The present results show that this task is a useful model to test recognition memory in mice and that blocking the central cholinergic system impairs this form of memory.
IMPLEMENTATION OF COMBINATIONAL CIRCUITS USING (2012)

This is the paper presenting a novel method for defining, analyzing and implementing the basic combinational circuitry with less number of ternary multiplexers. Multilplexer is used as basic building block to realize all the combinational and sequential circuitry providing complete, concise, implementation-free description of the ternary function involved. This shows the potential of VHDL modeling and simulation which can be applied to Ternary switching circuits for verifying its functionality and timing specifications. This is the method which is used in analyzing the complex ternary functions and reduction of gate count Keyword:- Gate count, Multivalued logic, Reabilityunreability model ,VHDL, Ternary switching levels.
Long-range phase-coherent effects in the transport properties of mesoscopic superconductor–normal-metal structures (1997)

We present a theory of long-range phase-coherent effects in the transport properties of a normal-metal film contacting two superconductors. It is shown that the phase-coherent correction to the conductance exists even though the spacing between the two superconductors largely exceeds the coherence length and the critical Josephson current is exponentially small. This effect can appear as a Shapiro step or large conductance oscillation caused by the magnetic field. Moreover, it is shown that in the case of negligible Josephson coupling between superconductors, the Josephson effect can arise in the system if an additional current flows through the normal conductor and a dissipation takes place (dissipative Josephson effect). The amplitude of the phase-coherent correction part of the conductance is discussed from the viewpoint of temperature, bias voltage, magnetic field, and phase-breaking length dependence.
The Scorpion Toxin Analogue BmKTX-D33H as a Potential Kv1.3 Channel-Selective Immunomodulator for Autoimmune Diseases (2016)

The Kv1.3 channel-acting scorpion toxins usually adopt the conserved anti-parallel β-sheet domain as the binding interface, but it remains challenging to discover some highly selective Kv1.3 channel-acting toxins. In this work, we investigated the pharmacological profile of the Kv1.3 channel-acting BmKTX-D33H, a structural analogue of the BmKTX scorpion toxin. Interestingly, BmKTX-D33H, with its conserved anti-parallel β-sheet domain as a Kv1.3 channel-interacting interface, exhibited more than 1000-fold selectivity towards the Kv1.3 channel as compared to other K⁺ channels (including Kv1.1, Kv1.2, Kv1.7, Kv11.1, KCa2.2, KCa2.3, and KCa3.1). As expected, BmKTX-D33H was found to inhibit the cytokine production and proliferation of both Jurkat cells and human T cells in vitro. It also significantly improved the delayed-type hypersensitivity (DTH) responses, an autoreactive T cell-mediated inflammation in rats. Amino acid sequence alignment and structural analysis strongly suggest that the "evolutionary" Gly11 residue of BmKTX-D33H interacts with the turret domain of Kv1 channels; it appears to be a pivotal amino acid residue with regard to the selectivity of BmKTX-D33H towards the Kv1.3 channel (in comparison with the highly homologous scorpion toxins). Together, our data indicate that BmKTX-D33H is a Kv1.3 channel-specific blocker. Finally, the remarkable selectivity of BmKTX-D33H highlights the great potential of evolutionary-guided peptide drug design in future studies.
Magnetic resonance contrast media: principles and progress. (1990)

The principles and current state of the art for magnetic resonance (MR) imaging contrast media are reviewed. All forms of paramagnetic and superparamagnetic MR contrast agents are covered, including discussions of their effect on MR relaxation and image intensity as well as their chemical and physiological properties.
Short‐ and long‐term survival and function of unilateral intrastriatal dopaminergic grafts in Parkinson's disease (1997)

Six patients with Parkinson's disease were followed for 10 to 72 months after human embryonic mesencephalic tissue from four to seven donors was grafted unilaterally into the putamen (4 patients) or putamen plus caudate (2 patients). After 8 to 12 months, positron emission tomography showed a 68% increase of 6-L-[18F]-fluorodopa uptake in the grafted putamen, no change in the grafted caudate, and minor decreases in nongrafted striatal regions. There was therapeutically valuable improvement in 4 patients, but only modest changes in the other 2, both of whom developed atypical features. Patient 4 was without L-dopa from 32 months and had normal fluorodopa uptake in the grafted putamen at 72 months. Overall, the L-dopa dose was reduced by a mean of 10 and 20%, "off" time was reduced by 34 and 44%, and the "off" phase Unified Parkinson's Disease Rating Scale motor score by 18 and 26%, and the duration of the response to a single L-dopa dose increased by 45 and 58% during the first and second years after surgery, respectively. Rigidity and hypokinesia improved bilaterally, but mainly contralateral to the implant. No consistent changes in dyskinesias were observed. We conclude that transplantation of embryonic mesencephalic tissue leads to highly reproducible survival of dopaminergic neurons, inducing clinically valuable improvements in most recipients.
Cumulative ultraviolet radiation flux in adulthood and risk of incident skin cancers in women (2014)

Associations with cumulative UV exposure in adulthood among women differed for melanoma, SCC, and BCC, suggesting a potential variable role of UV radiation in adulthood in the carcinogenesis of the three major skin cancers.
Cationic Lipids in Gene Delivery: Principles, Vector Design and Therapeutical Applications (2002)

Gene therapy will change medicine by treating the diseases at their core levels revolutionizing the way to deliver functional proteins. The development of this technology relies in designing optimal systems for DNA transfer and expression (transfection), cationic lipids being a promising alternative. Being safer than viral vectors, they also allow the delivery of larger plasmids and can be easily GMP-manufactured and stored. The main problem associated with the use of these vectors is their transfection efficiency, which is still inferior to viral methods. In this paper we present an overview of the correlations between the chemical structure and biological activity for the principal classes of cationic lipids. Key issues in the design of this class of transfection agents are presented, as well as the future trends.
Depth camera based hand gesture recognition and its applications in Human-Computer-Interaction (2011)

Of various Human-Computer-Interactions (HCI), hand gesture based HCI might be the most natural and intuitive way to communicate between people and machines, since it closely mimics how human interact with each other. Its intuitiveness and naturalness have spawned many applications in exploring large and complex data, computer games, virtual reality, health care, etc. Although the market for hand gesture based HCI is huge, building a robust hand gesture recognition system remains a challenging problem for traditional vision-based approaches, which are greatly limited by the quality of the input from optical sensors. [16] proposed a novel dissimilarity distance metric for hand gesture recognition using Kinect sensor, called Finger-Earth Mover's Distance (FEMD). In this paper, we compare the performance in terms of speed and accuracy between FEMD and traditional corresponding-based shape matching algorithm, Shape Context. And then we introduce several HCI applications built on top of a accurate and robust hand gesture recognition system based on FEMD. This hand gesture recognition system performs robustly despite variations in hand orientation, scale or articulation. Moreover, it works well in uncontrolled environments with background clusters. We demonstrate that this robust hand gesture recognition system can be a key enabler for numerous hand gesture based HCI systems.
Widespread Translational Inhibition by Plant miRNAs and siRNAs (2008)

High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE (VCS)/Ge-1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.
Upregulation of leptin receptor mRNA expression in obese mouse brain (1997)

Leptin receptor gene expression in the brains of lean (+/+) and obese (ob/ob) C57Bl/6 mice was examined using a non-radioactive in situ hybridization detection method. Significant increases in leptin receptor mRNA expression were found in the ventromedial and arcuate hypothalamic nuclei, piriform and olfactory cortices and medial habenular nucleus. There were very minor changes in the amount of leptin receptor mRNA expression in hippocampus proper (CA1-3). Results indicated that leptin receptor is upregulated when there is a lack of functional leptin, as in hereditary obese (ob/ob) mice. It is also suggested that leptin receptor may be an autoreceptor.
Proposals for the Classification of the Acute Leukaemias French‐American‐British (FAB) Co‐operative Group (1976)

A uniform system of classification and nomenclature of the acute leukaemias, at present lacking, should permit more accurate recording of the distribution of cases entered into clinical trials, and could provide a reference standard when newly developed cell-surface markers believed to characterize specific cell types are applied to cases of acute leukaemia. Proposals based on conventional morphological and cytochemical methods are offered following the study of peripheral blood and bone-marrow films from some 200 cases of acute leukaemia by a group of seven French, American and British haematologists. The slides were examined first independently, and then by the group working together. Two groups of acute leukaemia, 'lymphoblastic' and myeloid are further subdivided into three and six groups. Dysmyelopoietic syndromes that may be confused with acute myeloid leukaemia are also considered. Photomicrographs of each of the named conditions are presented.
Therapeutic limitations in tumor-specific CD8+ memory T cell engraftment (2003)

Memory engraftment with tumor-specific CTL provides long-term protection against tumor. However, there are several limitations to this immunotherapeutic strategy, especially when targeting a single antigen. This study illustrates the importance of administering large numbers of effectors to engraft sufficiently efficacious immunologic memory. It also demonstrates the importance of targeting several antigens when developing vaccine strategies for cancer.
Stable silver nanoparticle–DNA conjugates for directed self-assembly of core-satellite silver–gold nanoclusters (2009)

We report a novel strategy to functionalize silver nanoparticles with chimeric phosphorothioate modified DNA (ps-po-DNA) and the fabrication of bimetallic core-satellite nanoclusters that each contain a silver core (diameter 32 nm) surrounded by 5 nm gold NPs.
Synchronizing network probes to avoid measurement intrusiveness with the Network Weather Service (2002)

Presents a scalable protocol for conducting periodic probes of network performance in a way that minimizes collisions between separate probes. The goal of the protocol is to enable active performance monitoring of large-scale distributed computational systems and networks. We use the protocol to generate time series of measurement data that are then exposed to numerical forecasting models when a prediction of network performance is required. We present the protocol and demonstrate its effectiveness using the Network Weather Service -a tool for dynamically predicting network, CPU, memory and storage performance.
Robust audio watermarking in the time domain (2001)

The audio watermarking method proposed in this paper offers copyright protection to an audio signal by time domain processing. The strength of audio signal modifications is limited by the necessity to produce an output signal that is perceptually similar to the original one. The watermarking method presented here does not require the use of the original signal for watermark detection. The watermark signal is generated using a key, i.e., a single number known only to the copyright owner. Watermark embedding depends on the audio signal amplitude and frequency in a way that minimizes the audibility of the watermark signal. The embedded watermark is robust to common audio signal manipulations like MPEG audio coding, cropping, time shifting, filtering, resampling, and requantization.
A Combination Vaccine for Allergy and Rhinovirus Infections Based on Rhinovirus-Derived Surface Protein VP1 and a Nonallergenic Peptide of the Major Timothy Grass Pollen Allergen Phl p 1 (2009)

Allergens and rhinovirus infections are among the most common elicitors of respiratory diseases. We report the construction of a recombinant combination vaccine for allergy and rhinovirus infections based on rhinovirus-derived VP1, the surface protein which is critically involved in infection of respiratory cells, and a nonallergenic peptide of the major grass pollen allergen Phl p 1. Recombinant hybrid molecules consisting of VP1 and a Phl p 1-derived peptide of 31 aa were expressed in Escherichia coli. The hybrid molecules did not react with IgE Abs from grass pollen allergic patients and lacked allergenic activity when exposed to basophils from allergic patients. Upon immunization of mice and rabbits, the hybrids did not sensitize against Phl p 1 but induced protective IgG Abs that cross-reacted with group 1 allergens from different grass species and blocked allergic patients' IgE reactivity to Phl p 1 as well as Phl p 1-induced basophil degranulation. Moreover, hybrid-induced IgG Abs inhibited rhinovirus infection of cultured human epithelial cells. The principle of fusing nonallergenic allergen-derived peptides onto viral carrier proteins may be used for the engineering of safe allergy vaccines which also protect against viral infections.
Deletion of the Proline-Rich Region of the Murine Metastasis Susceptibility Gene Brd4 Promotes Epithelial-to-Mesenchymal Transition- and Stem Cell-Like Conversion (2011)

The bromodomain-containing chromatin-modifying factor BRD4 is an inherited susceptibility gene for breast cancer progression and metastasis, but its functionality in these settings has yet to be explored. Here we show that deletion of either of the BRD4 bromodomains had modest effects on the metastatic suppression ability of BRD4. In contrast, expression of the natural short isoform of BRD4 that truncates the protein after the SEED domain restored progression and metastatic capacity. Unexpectedly, deletion of the proline-rich region induced mesenchymal-like conversion and acquisition of cancer stem cell-like properties, which are mediated by the carboxy-terminal P-TEFb binding domain. Deletion of this proline-rich region also induced a gene expression signature that predicted poor outcome in human breast cancer data sets and that overlapped G3 grade human breast tumors. Thus our findings suggest that BRD4 may be altering the predisposition of tumors to undergo conversion to a more de-differentiated or primitive state during metastatic progression.
Perception and production of English fricative sounds by advanced Korean EFL learners (2011)

This study explored the perception and production of English voiceless fricatives among advanced Korean EFL learners by conducting on-line experiments. Thirty two college students who had overall high English proficiency participated in the experiments and nonce words beginning with one of the voiceless fricatives /f/, /?/, /s/, and /?/ were used in the experiments. In particular, 48 pairs of English nonce words, 24 identical (e.g., findert-findert, thomber-thomber) and non-identical pairs (e.g., findert-thindert, thomber-fomber) each, were employed in the perception test and the same 24 nonce words (e.g., findert, thimbert, simbert, shipkin; fomber, thombul, sombul, sholtem) were used in the production test. The results showed the precedence of perception over production but no correlation between them, indicating that the learners’ perceptual deficiency of target fricatives was not necessarily tied to their production defect of those sounds or vice versa. Importantly, the results from the perception test revealed that the participants had a great difficulty with the /f/-/?/ contrast, as opposed to the /s/-/?/ contrast. The participants’ ability to contrast between /?/ and /s/ was relatively good, unlike findings of previous studies (Joh and Lee 2001). As for production, the participants had most difficulty with the interdental fricative / / followed by /f/, but they did not have much difficulty with /s/ or /?/. Even though the results of the production test are overall consistent with those of the perception test, some discrepancy was found especially in the perception and production of /f/ and /?/. Further, replacement patterns in production were analyzed in terms of phonetic and/or articulatory properties of the target sounds and the L1 phonemic inventory. Some implications for the teaching of fricative sounds were also drawn.
Expression pattern of four membrane‐type matrix metalloproteinases in the normal and diseased mouse mammary gland (2005)

Both mammary gland development and mammary carcinogenesis involve extensive remodeling of the mammary gland extracellular matrix. The expression of four membrane-type matrix metalloproteinases (MT-MMPs) with matrix remodeling potential in development and tumorigenesis was evaluated by in-situ hybridization on mouse mammary gland sections. MT1-MMP and MT3-MMP were found in the mammary stroma mainly around epithelial structures in both developing and mature mammary gland. In contrast, MT2-MMP was found exclusively in the mammary epithelium. Lactating gland expressed none of the examined MT-MMPs. Mammary gland tumors expressed MT1-MMP, MT2-MMP, and MT3-MMP while MT4-MMP was not expressed in any developmental or cancerous stage analyzed here. Our results suggest that MT1-MMP, MT2-MMP, and MT3-MMP may be involved in remodeling of both the normal and diseased mammary gland either directly or indirectly by activation of other MMPs.
Characterization of the influenza virus M2 integral membrane protein and expression at the infected-cell surface from cloned cDNA (1985)

An investigation of properties of the influenza A virus M2 protein indicated that it is synthesized by 2 h postinfection together with other viral polypeptides and is transported to the infected-cell surface with a half-time of approximately 30 to 40 min. The available evidence suggests that M2 is not N-glycosylated even though it contains a potential glycosylation site, and the intracellular pattern of protein distribution includes localization to the Golgi apparatus. Proteolysis of intracellular microsome vesicles followed by immunoprecipitation with antiserum to a synthetic oligopeptide indicated that the M2 protein contains an extensive region of COOH-terminal amino acids exposed on the cytoplasmic side of the infected-cell membrane. A cDNA clone of the M2 mRNA was obtained and expressed in an SV40 recombinant vector. The M2 protein expressed by the vector became associated with the Golgi complex and was found on the surface of vector-infected cells. M2 is antigenically conserved among all strains of influenza virus both in regions exposed on the cell surface and intracellularly.
Tyrosyl-DNA Phosphodiesterase 1 (Tdp1) inhibitors (2011)

Inhibitors of topoisomerase I (Top1) that result in stalled Top1 cleavage complexes (Top1cc) are commonly employed against cancer. Combination chemotherapy with DNA repair inhibitors can potentially improve response to these widely used chemotherapeutics. One line of inquiry focuses on inhibitors of tyrosyl-DNA phosphodiesterase 1 (Tdp1), a repair enzyme for Top1cc. Tdp1 catalyzes the hydrolysis of DNA adducts covalently linked to the 3'-phosphate of DNA, including Top1-derived peptides and also 3'-phosphoglycolates. Tdp1 inhibitors should synergize not only with Top1-targeting drugs (camptothecins, indenoisoquinolines), but also with bleomycin, topoisomerase II (Top2) inhibitors (etoposide, doxorubicin) and DNA alkylating agents. Here, we summarize the structure-activity relationship obtained from the reported Tdp1 inhibitors. Better understanding of Top1cc repair in vivo coupled with detailed structural studies on Tdp1-inhibitor interaction will be crucial in guiding the rational design of Tdp1 inhibitors.
Ammonia Saturation Constants for Predominant Species of Rumen Bacteria (1980)

Ammonia saturation constants were determined for representative pure cultures of predominant, anaerobic, fermentative rumen bacteria. Based on growth experiments with ammonia limited continuous cultures, average estimates for ammonia saturation constants of Bacteroides amylophillus and Bacteroides ruminicola were 10.5 and 23.5 microM ammonia-nitrogen, respectively. With ammonia-limited linear-growth cultures, the estimates for the ammonia saturation constants of B. amylophilus, B. ruminicola, and Selenomonas ruminantium were, respectively, 6, 33.5, and 18 microM ammonia nitrogen. By a third method, which involved estimation of ammonia concentration in the medium when the growth rate of ammonia-limited batch cultures reached half maximal, the ammonia saturation constant was determined for the species mentioned as well as Megaspaera elsdenii and Ruminococcus flavefaciens. Except for M. elsdenii, saturation constants of the other bacteria were less than 50 microM ammonia nitrogen. An organism with a saturation constant for ammonia of 50 microM growing in a medium containing 1 mM ammonia should achieve 95% of its maximum specific growth rate. Many of the predominant species of rumen bacteria are efficient scavengers of ammonia.
Rational Polytherapy with Antiepileptic Drugs (2010)

Approximately 30-40% of patients do not achieve seizure control with a single antiepileptic drug (AED). With the advent of multiple AEDs in the past 15 years, rational polytherapy, the goal of finding combinations of AEDs that have favorable characteristics, has become of greater importance. We review the theoretical considerations based on AED mechanism of action, animal models, human studies in this field, and the challenges in finding such optimal combinations. Several case scenarios are presented, illustrating examples of rational polytherapy.