gebhart/patrepeval huggingface dataset:
Diagnostic value of C-reactive protein in acute appendicitis (1994)

Serum C-reactive protein was measured in 56 patients hospitalized with a suspected diagnosis of acute appendicitis. Based on these determinations, four groups of patients were defined: Group A = 26 patients with acute appendicitis who had a C-reactive protein level higher than 2.5 mg/dl. Group B = 4 patients with a C-reactive protein level lower than 2.5 mg/dl who, after surgery based on a presumed diagnosis of acute appendicitis, were found to have a normal appendix. Group C = 22 patients with nonspecific abdominal pain, 18 (72 percent) of whom had an elevated C-reactive protein level, although in only 4 (7.1 percent) were these levels higher than 2.5 percent mg/dl. Group D = 4 patients who had diseases other than acute appendicitis. It is concluded that an increase in C-reactive protein levels to more than 2.5 mg/dl is not a definite indicator of acute appendicitis. However, if the C-reactive protein level in blood drawn 12 hours after the onset of symptoms is less than 2.5 mg/dl, acute appendicitis can be excluded.
Coupled Isotachophoretic Preconcentration and Electrophoretic Separation Using Bidirectional Isotachophoresis (2011)

We present a novel technique for coupling isotachophoretic preconcentration and electrophoretic separation using bidirectional isotachophoresis (ITP). Bidirectional ITP simultaneously sets up sharp ITP interfaces between relatively high- and low-mobility cations and high- and low-mobility anions. These two interfaces can migrate toward each other and be described as ion concentration shock waves. We here demonstrate a bidirectional ITP process in which we use the interaction of these anionic and cationic ITP shock waves to trigger a transformation from ITP preconcentration to electrophoretic separation. We use anionic ITP to focus anionic sample species prior to shock interaction. The interaction of the counter-propagating anionic and cationic ITP shocks then changes the local pH (and ionic strength) of the focused analyte zones. Under this new condition, the analytes no longer focus and begin to separate electrophoretically. The method provides faster and much less dispersive transition from ITP preconcentration to electrophoretic separation compared with traditional (unidirectional) transient ITP. It eliminates the need for intermediate steps between focusing and separation, such as manual buffer exchanges. We illustrate the technique with numerical simulations of species transport equations. We have validated our simulations with experimental visualization of bidirectional ITP zones. We then show the effectiveness of the technique by coupling ITP preconcentration and high-resolution separation of a 1 kbp DNA ladder via shock interaction in bidirectional ITP.
ChemInform Abstract: STUDIES OF KETENE AND ITS DERIVATIVES. PART 119. REACTIONS OF HALOKETENES WITH 2‐ARYLIDENEAMINOPYRIDINES (1984)

Abstract Dichlorketen (II), das aus Dichloracetylchlorid in situ mit Triethylamin erzeugt wird, addiert an die Benzaldimine (I) je nach Struktur zu acyclischen und cyclischen Addukten (III)‐(V).
Chemoenzymatic Synthesis and Lectin Array Characterization of a Class of N-Glycan Clusters (2009)

N-Glycans are major components of many glycoproteins. These sugar moieties are frequently involved in important physiological and disease processes via their interactions with a variety of glycan-binding proteins (GBP). Clustering effect is an important feature in many glycan-lectin interactions. We describe in this paper a chemoenzymatic synthesis of novel N-glycan clusters using a tandem endoglycosidase-catalyzed transglycosylation. It was found that the internal beta-1,2-linked GlcNAc moieties in the N-glycan core, once exposed in the nonreducing terminus, was able to serve as acceptors for transglycosylation catalyzed by Endo-A and EndoM-N175A. This efficient chemoenzymatic method allows a quick extension of the sugar chains to form a class of glycan clusters in which sugar residues are all connected by native glycosidic linkages found in natural N-glycans. In addition, a discriminative enzymatic reaction at the two GlcNAc residues could be fulfilled to afford novel hybrid clusters. Lectin microarray studies revealed unusual properties in glyco-epitope expression by this panel of structurally well-defined synthetic N-glycans. These new compounds are likely valuable for functional glycomics studies to unveil new functions of both glycans and carbohydrate-binding proteins.
In Pursuit of Carbohydrate‐Based HIV Vaccines, Part 2: The Total Synthesis of High‐Mannose‐Type gp120 Fragments—Evaluation of Strategies Directed to Maximal Convergence (2004)

A “layered approach” and a “block approach” were used to assemble the high-mannose glycan of gp120 glycopeptide fragments (e.g. high-mannose-type conjugates gp120316–335 1). The glycan was then conjugated with the gp120 peptide segments through direct aspartylation. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z53626_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Liquid crystal spatial light modulators for optical interconnects and space switching (1990)

Many schemes for parallel optical interconnects involve the use of spatial light modulators (SLMs). Advances have occurred in liquid crystal technology that promise major improvements in the performance of SLMs. These are not based on electro-optic effects in the familiar nematic and cholesteric phases, but on a number of newer electro-optic effects in chiral smectic liquid crystals. The authors examine two of these electro-optic effects; the so called ferroelectric switching in chiral smectic C liquid crystals and electronic switching in chiral smectic A phases. They assess their prospects in electrically addressed spatial light modulators and emphasise the problems of obtaining in large arrays of pixels the same performance as can be obtained from single pixels. A hybrid technology, combining chiral smectic liquid crystals and silicon VLSI, is proposed as a means of obtaining addressable structures of large arrays of pixels. The problems and prospects of this hybrid technology are briefly reviewed. >
Characterization of Activity and Expression of Isocitrate Lyase in Mycobacterium avium and Mycobacterium tuberculosis (1999)

Analysis by two-dimensional gel electrophoresis revealed that Mycobacterium avium expresses several proteins unique to an intracellular infection. One abundant protein with an apparent molecular mass of 50 kDa was isolated, and the N-terminal sequence was determined. It matches a sequence in the M. tuberculosis database (Sanger) with similarity to the enzyme isocitrate lyase of both Corynebacterium glutamicum and Rhodococcus fascians. Only marginal similarity was observed between this open reading frame (ORF) (termed icl) and a second distinct ORF (named aceA) which exhibits a low similarity to other isocitrate lyases. Both ORFs can be found as distinct genes in the various mycobacterial databases recently published. Isocitrate lyase is a key enzyme in the glyoxylate cycle and is essential as an anapleurotic enzyme for growth on acetate and certain fatty acids as carbon source. In this study we express and purify Icl, as well as AceA proteins, and show that both exhibit isocitrate lyase activity. Various known inhibitors for isocitrate lyase were effective. Furthermore, we present evidence that in both M. avium and M. tuberculosis the production and activity of the isocitrate lyase is enhanced under minimal growth conditions when supplemented with acetate or palmitate.
Unprecedented host-induced intramolecular charge-transfer complex formationElectronic supplementary information (ESI) available: synthetic procedure and characterization data of 1 and 2. See http://www.rsc.org/suppdata/cc/b2/b208280b/ (2002)

For the first time, host-induced intramolecular charge-transfer complex formation in a guest containing both an electron donor and an electron acceptor is demonstrated in the cucurbit[8]uril cavity, leading to unusual back-folding of the guest molecule.
Impaired Cl−Extrusion in Layer V Pyramidal Neurons of Chronically Injured Epileptogenic Neocortex (2005)

In the mature brain, the K(+)/Cl- cotransporter KCC2 is important in maintaining low [Cl-]i, resulting in hyperpolarizing GABA responses. Decreases in KCC2 after neuronal injuries result in increases in [Cl-]i and enhanced neuronal excitability due to depolarizing GABA responses. We used the gramicidin perforated-patch technique to measure E(Cl) ( approximately E(GABA)) in layer V pyramidal neurons in slices of partially isolated sensorimotor cortex of adult rats to explore the potential functional consequence of KCC2 downregulation in chronically injured cortex. E(GABA) was measured by recording currents evoked with brief GABA puffs at various membrane potentials. There was no significant difference in E(Cl) between neurons in control and undercut animals (-71.2 +/- 2.6 and -71.8 +/- 2.8 mV, respectively). However, when loaded with Cl- by applying muscimol puffs at 0.2 Hz for 60 s, neurons in the undercut cortex had a significantly shorter time constant for the positive shift in E(Cl) during the Cl- loading phase (4.3 +/- 0.5 s for control and 2.2 +/- 0.4 s for undercut, P < 0.01). The positive shift in E(Cl) 3 s after the beginning of Cl- loading was also significantly larger in the undercut group than in the control, indicating that neurons in undercut cortex were less effective in maintaining low [Cl-]i during repetitive activation of GABA(A) receptors. Application of furosemide eliminated the difference between the control and undercut groups for both of these measures of [Cl-]i regulation. The results suggest an impairment in Cl- extrusion resulting from decreased KCC2 expression that may reduce the strength of GABAergic inhibition and contribute to epileptogenesis.
Where do emotions come from? Predicting the Emotion Stimuli Map (2016)

Which parts of an image evoke emotions in an observer? To answer this question, we introduce a novel problem in computer vision - predicting an Emotion Stimuli Map (ESM), which describes pixel-wise contribution to evoked emotions. Building a new image database, EmotionROI, as a benchmark for predicting the ESM, we find that the regions selected by saliency and objectness detection do not correctly predict the image regions which evoke emotion. Although objects represent important regions for evoking emotion, parts of the background are also important. Based on this fact, we propose using fully convolutional networks for predicting the ESM. Both qualitative and quantitative experimental results confirm that our method can predict the regions which evoke emotion better than both saliency and objectness detection.
Pathways for angiotensin-(1—7) metabolism in pulmonary and renal tissues (2000)

Two of the primary sites of actions for angiotensin (ANG)-(1---7) are the vasculature and the kidney. Because little information exists concerning the metabolism of ANG-(1---7) in these tissues, we investigated the hydrolysis of the peptide in rat lung and renal brush-border membrane (BBM) preparations. Radiolabeled ANG-(1---7) was hydrolyzed primarily to ANG-(1---5) by pulmonary membranes. The ANG-converting enzyme (ACE) inhibitor lisinopril abolished the generation of ANG-(1---5), as well as that of smaller metabolites. Kinetic studies of the hydrolysis of ANG-(1---7) to ANG-(1---5) by somatic (pulmonary) and germinal (testes) forms of rat ACE yielded similar values, suggesting that the COOH-domain is responsible for the hydrolysis of ANG-(1---7). Pulmonary metabolism of ANG-(1---5) yielded ANG-(3---5) and was independent of ACE but may involve peptidyl or dipeptidyl aminopeptidases. In renal cortex BBM, ANG-(1---7) was rapidly hydrolyzed to mono- and dipeptide fragments and ANG-(1---4). Aminopeptidase (AP) inhibition attenuated the hydrolysis of ANG-(1---7) and increased ANG-(1---4) formation. Combined treatment with AP and neprilysin (Nep) inhibitors abolished ANG-(1---4) formation and preserved ANG-(1---7). ACE inhibition had no effect on the rate of hydrolysis or the metabolites formed in the BBM. In conclusion, ACE was the major enzymatic activity responsible for the metabolism of ANG-(1---7) in the lung, which is consistent with the ability of ACE inhibitors to increase the half-life of circulating ANG-(1---7) and raise endogenous levels of the peptide. An alternate pathway of metabolism was revealed in the renal cortex, where increased AP and Nep activities, relative to ACE activity, promote conversion of ANG-(1---7) to ANG-(1---4) and smaller fragments.
Fetal rat septal cells adhere to and extend processes on basement membrane, laminin, and a synthetic peptide from the laminin A chain sequence (1991)

Responses of rat embryonic septal cells to reconstituted basement membrane, laminin, and laminin A chain-derived synthetic peptides were studied in culture. Dissociated fetal E16/17 septal cells were grown for three days on differently coated plastic substrata. Reconstituted basement membrane (Matrigel), laminin, and a 19-amino acid synthetic peptide CSRARKQAASIKVAVSADR-NH2 (PA22-2) from the laminin A chain sequence mediated cell-substratum adhesion and promoted neurite outgrowth. In contrast, cells did not attach to or form processes on uncoated plastic or on plastic substrata coated with synthetic, laminin-derived control peptides. Polyethylenimine (PEI) supported the adhesion and survival of fetal septal cells; however, when laminin was added to the medium during cell plating or 18 hr afterward, a dose-dependent increase was observed in neurite outgrowth of cells attached to this substratum. Cells grown for 6 days on PEI in the presence of laminin showed a determined increase in the number of cholinergic neurons as marked by acetylcholinesterase staining. These data suggest that the subpopulation of cholinergic septal neurons present in the septal cells studied here were also responding to laminin. The results of this in vitro study suggest potential uses for basement membrane, laminin, or synthetic peptides, such as PA22-2, in fetal septal grafts to enhance regeneration in the damaged septo-hippocampal system.
Individualized Developmental Care for the Very Low-Birth-Weight Preterm Infant (1994)

Very low-birth-weight preterm infants may benefit from individualized developmental care in the neonatal intensive care unit in terms of medical and neurodevelopmental outcome.
Risk Factors of Suicidal Phenomenon: Prevention and Intervention (2014)

Around the world suicide has caused more deaths per year than homicide or war (World Health Organization, 2002). Suicidal attempts (the person survives) and suicidal ideation (thinking seriously about suicide) are others dimensions of the suicide phenomenon. A number of risk factors have been considered as factors contributing to the increased likelihood of suicidal ideation, attempts, and completions. Sociological (external) factors and psychological (internal) factors have been considered in increasing suicidal risk. Beyond the individual factors research has also explained the family, social, and community aspects of the suicidal phenomenon. Helping professionals planning suicide intervention and prevention strategies need to be aware of both the myths and misperceptions of the suicide phenomenon, as well as research based risk factors.
An integrated voice/data system for mobile indoor radio networks using multiple transmission rate (2003)

A centralized, integrated voice/data radio network for indoor fading multipath radio channels is proposed and analyzed. It can operate as a stand-alone system or as a cell in a cellular network. The packets of voice and data in the uplink are integrated through a movable-boundary framed-polling protocol to optimize the throughput of the data traffic for a given number of voice terminals. The down link is a simple TDM (time division multiplexing) system. The system dynamically switches between two transmission rates and uses multiple antennas to maximize the throughput in the fading multipath indoor channel. Using three different techniques, the throughput and delay characteristic of the system are analyzed. A simple equation is given to provide an approximate relation between the number of voice users, the data traffic with less than 10-ms delay, and the average transmission rate of the system. The results are compared with those of Monte Carlo computer simulations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Real‐Time PCR (2008)

Abstract Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise quantification of specific nucleic acids in a complex mixture by fluorescent detection of labeled PCR products. Detection can be accomplished using specific, as well as nonspecific fluorescent probes. Real‐time PCR is often used in the quantification of gene expression levels. Prior to using real‐time PCR to quantify a target message, care must be taken to optimize the RNA isolation, primer design, and PCR reaction conditions so that accurate and reliable measurements can be made. This short overview of real‐time PCR discusses basic principles behind real‐time PCR, some optimization and experimental design considerations, and how to quantify the data generated using both relative and absolute quantification approaches. Useful Web sites and texts that expand upon topics discussed are also listed.
The future of peripheral nerve neurostimulation (2000)

Electrical stimulation of selected peripheral nerves for treatment of intractable pain has been used inconsistently over the past 30 years due to difficulties in clarifying appropriate indications, utilizing approved device technology, and standardizing the surgical techniques. Circumferential electrodes treating mononeuropathies have given way to paddle electrode techniques and, most recently, the application of percutaneous wire electrode methods will allow for minimally invasive peripheral nerve stimulation for certain intractable CRPS and other painful monoeuropathies.
Dynamic model evolution (2010)

In the Adaptive Object-Model (AOM) architectural style, user-defined domain entities and their relationships, attributes and behavior are described by externally stored metadata. AOM implementations reify the domain entities at runtime by interpreting the metadata. Users change the object model by editing the metadata to reflect changes in the domain. This paper describes a pattern for incorporating model changes into an AOM production system without taking the system off-line. Dynamic evolution of the object model is done during loading of a domain object. The Dynamic Model Evolution pattern is applicable when a system needs to keep running, and the alternative of updating the entire object model at the time a new version comes online is too time consuming to be a practical option.
Adaptive Language and Translation Models for Interactive Machine Translation (2004)

We describe experiments carried out with adaptive language and translation models in the context of an interactive computer-assisted translation program. We developed cache-based language models which were then extended to the bilingual case for a cache-based translation model. We present the improve-ments we obtained in two contexts: in a theoretical setting, we achieved a drop in perplexity for the new models and, in a more practical situation simulat-ing a user working with the system, we showed that fewer keystrokes would be needed to enter a trans-lation. 1
Real-time 3D and 4D Fourier domain Doppler optical coherence tomography based on dual graphics processing units (2012)

We present real-time 3D (2D cross-sectional image plus time) and 4D (3D volume plus time) phase-resolved Doppler OCT (PRDOCT) imaging based on configuration of dual graphics processing units (GPU). A GPU-accelerated phase-resolving processing algorithm was developed and implemented. We combined a structural image intensity-based thresholding mask and average window method to improve the signal-to-noise ratio of the Doppler phase image. A 2D simultaneous display of the structure and Doppler flow images was presented at a frame rate of 70 fps with an image size of 1000 × 1024 (X × Z) pixels. A 3D volume rendering of tissue structure and flow images-each with a size of 512 × 512 pixels-was presented 64.9 milliseconds after every volume scanning cycle with a volume size of 500 × 256 × 512 (X × Y × Z) voxels, with an acquisition time window of only 3.7 seconds. To the best of our knowledge, this is the first time that an online, simultaneous structure and Doppler flow volume visualization has been achieved. Maximum system processing speed was measured to be 249,000 A-scans per second with each A-scan size of 2048 pixels.
Colony-stimulating factor-induced monocyte survival and differentiation into macrophages in serum-free cultures. (1987)

The role of mononuclear phagocyte-specific colony-stimulating factor (CSF-1) in human monocyte to macrophage differentiation was investigated. The addition of 1000 U/ml of CSF-1 to serum-free monocyte cultures resulted in monocyte survival comparable to that in cultures containing 5% AB serum, whereas cells in serum- and CSF-1-free medium lost their viability in 3 to 5 days. The requirement for CSF-1 coincided with the time (40 to 64 hr of culture) when the major changes in morphology and biochemical function took place in monocytes undergoing differentiation into macrophages. If CSF-1 was removed from the cultures before this time, death of the monocytes resulted. In cultures containing CSF-1, as in serum containing cultures, the lysosomal enzyme acid phosphatase was enhanced 10- to 20-fold by day 4 to 5. Superoxide production in response to phorbol myristic acetate was maintained in CSF-1 cultured monocytes, but declined with time in monocytes cultured in serum. The expression of monocyte-macrophage antigens p150.95 (LeuM5), OKM1, LeuM3, Fc receptors (32.2), and HLA-DR had increased in CSF-1 containing cultures at day 4. When antigen expression was analyzed at day 2 to 3, when cell size and 90 degrees scatter characteristics were still identical to control serum-free cultures, only p150.95, HLA-DR and FcR expression were enhanced by CSF-1. Low amounts of lipopolysaccharide (0.1 ng/ml) were found to enhance monocyte survival in the absence of added CSF-1. Lipopolysaccharide-containing cultures were found to produce CSF-1 (up to 450 U/ml, as detected by radioimmunoassay). Lipopolysaccharide (1 microgram/ml), however, did not induce enhanced expression of the maturation-related antigens. Based on these observations we conclude that CSF-1 is enhancing human monocyte survival and is involved in the events leading to the differentiation of monocytes into macrophages.