gebhart/patrepeval huggingface dataset:
Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes (2001)

The study of human cardiac tissue development is hampered by the lack of a suitable in vitro model. We describe the phenotypic properties of cardiomyocytes derived from human embryonic stem (ES) cells. Human ES cells were cultivated in suspension and plated to form aggregates termed embryoid bodies (EBs). Spontaneously contracting areas appeared in 8.1% of the EBs. Cells from the spontaneously contracting areas within EBs were stained positively with anti–cardiac myosin heavy chain, anti–α-actinin, anti-desmin, anti–cardiac troponin I (anti-cTnI), and anti-ANP antibodies. Electron microscopy revealed varying degrees of myofibrillar organization, consistent with early-stage cardiomyocytes. RT-PCR studies demonstrated the expression of several cardiac-specific genes and transcription factors. Extracellular electrograms were characterized by a sharp component lasting 30 ± 25 milliseconds, followed by a slow component of 347 ± 120 milliseconds. Intracellular Ca2+ transients displayed a sharp rise lasting 130 ± 27 milliseconds and a relaxation component lasting 200–300 milliseconds. Positive and negative chronotropic effects were induced by application of isoproterenol and carbamylcholine, respectively. In conclusion, the human ES cell–derived cardiomyocytes displayed structural and functional properties of early-stage cardiomyocytes. Establishment of this unique differentiation system may have significant impact on the study of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering.
The rise of single-molecule DNA biochemistry (1998)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Studies on the Leukocyte-common Antigen: Structure, Function, and Evolutionary Conservation (1989)

The leukocyte-common antigen (L-CA, CD45) is a family of large-molecular-weight glycoproteins expressed on the surface of all cells of hematopoietic origin except erythrocytes and platelets. The function of this family of molecules has not been well understood; however, studies using antibodies against L-CA have implicated the family in lymphocyte activation and proliferation (for review, see Thomas 1989). We recently developed a mouse T-cell clone that is deficient in the surface expression of L-CA (Pingel and Thomas 1989). This clone is impaired in certain aspects of proliferation, implicating L-CA in the regulation of lymphocyte cell division. Here, we correlate our functional studies with our previous results on the structure of the L-CA family and present a model to account for these results.
A Comparative Study of Technology Options for Next Generation Intra- and Inter-datacenter Interconnects (2018)

We review signaling schemes, whose receiver relies on signal self-beating or signal beating with accompanying CW tone, and compare their transceiver architectures, spectral efficiencies, receiver bandwidths and enabling digital signal processing.
The reconstruction of a multidimensional sequence from the phase or magnitude of its Fourier transform (1982)

This paper addresses two fundamental issues involved in the reconstruction of a multidimensional sequence from either the phase or magnitude of its Fourier transform. The first issue relates to the uniqueness of a multidimensional sequence in terms of its phase or magnitude. Although phase or magnitude information alone is not sufficient, in general, to uniquely specify a sequence, a large class of sequences are shown to be recoverable from their phase or magnitude. The second issue which is addressed in this paper concerns the actual reconstruction of a multidimensional sequence from its phase or magnitude. For those sequences which are uniquely specified by their phase, several practical algorithms are described which may be used to reconstruct a sequence from its phase. Several examples of phase-only reconstruction are also presented. Unfortunately, however, even for those sequences which are uniquely defined by their magnitude, it appears that a practical algorithm is yet to be developed for reconstructing a sequence from only its magnitude. Nevertheless, an iterative procedure which has been proposed is briefly discussed and evaluated.
A Natural Product That Lowers Cholesterol As an Antagonist Ligand for FXR (2002)

Extracts of the resin of the guggul tree (Commiphora mukul) lower LDL (low-density lipoprotein) cholesterol levels in humans. The plant sterol guggulsterone [4,17(20)-pregnadiene-3,16-dione] is the active agent in this extract. We show that guggulsterone is a highly efficacious antagonist of the farnesoid X receptor (FXR), a nuclear hormone receptor that is activated by bile acids. Guggulsterone treatment decreases hepatic cholesterol in wild-type mice fed a high-cholesterol diet but is not effective in FXR-null mice. Thus, we propose that inhibition of FXR activation is the basis for the cholesterol-lowering activity of guggulsterone. Other natural products with specific biologic effects may modulate the activity of FXR or other relatively promiscuous nuclear hormone receptors.
Hollow nanostructures from self-assembled supramolecular metallo-triblock copolymers (2009)

A reversible addition fragmentation chain transfer (RAFT) agent functionalised with an SCS ‘pincer’ ligand was synthesised and shown to control the polymerisation of methyl acrylate to afford chain-end pincer-functionalised poly(methyl acrylate) (PMA). This was then chain-extended with tert-butyl acrylate (tBuA) to afford a diblock (PtBuA-b-PMA) end-functionalised with a pincer ligand, with good control over the resultant polymer’s molecular weight and polydispersity. This diblock copolymer was chain-end-complexed using a palladium(II) precursor and then deprotected to afford a diblock copolymer chain-end-functionalised with an amphiphilic pincer ligand Pd(II) complex (PAA-b-PMA-Pd). The chain-end binding of this polymer with a second, pyridine-end-functionalised poly(styrene) (PS) block proceeded in a facile manner using recently established chemistries to afford an unsymmetrical amphiphilic metallo-triblock copolymer (PAA-b-PMA-Pd-PS). The self-assembly of this triblock copolymer into monodisperse layered non-covalently connected micelles (NCCMs) and non-covalently connected nanoparticles (NCCNs) via covalent shell crosslinking was evidenced by DLS, TEM and AFM analysis. The hydrophobic PS core domain was then detached from the shell crosslinked layer via protonation of the pyridine moiety at low pH and the core was removed viadialysis to give hollow cage-like nanostructures whose interiors retain their hydrophobicity and contain functional Pd(II) complexes.
Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy (2008)

In past years, both laboratory and early clinical studies have demonstrated that biospecific antibodies (BsAbs) may have significant potential application in cancer therapy either by targeting tumor cells with cytotoxic agents including effector cells, radionuclides, drugs, and toxins, or by simultaneously blocking two relevant tumor targets, that is, growth factor receptors, thus neutralizing multiple receptor activation and downstream signal transduction pathways. A major obstacle in the development of BsAb has been the difficulty of producing the materials in sufficient quality and quantity by traditional technologies such as the hybrid hybridoma and chemical conjugation methods. The development of IgG-like BsAbs as therapeutic agents will depend heavily on the advances made in the design of recombinant BsAb constructs (or formats) and production efficiency. Here we describe a recombinant method for the construction and production of a novel IgG-like BsAb molecule, using the variable domains of two fully human antibodies as the building blocks.
Time-resolved optical imaging through turbid media using a fast data acquisition system based on a gated CCD camera (2003)

In this paper, we propose a novel approach for the acquisition of time-resolved data for optical tomography. A fast gated CCD has been used as a parallel detector to acquire in one shot the light intensity exiting a phantom within a very short time slice. By using a pulsed illumination and repeating the acquisition at different delays, the time behaviour of the diffused transmittance can be recorded very quickly. Scattering inclusions embedded in a 5 cm thick phantom have been revealed by fitting a set of 120 images, delayed 50 ps from one another, with a mathematical model based on the random walk theory. Moreover, absorption inclusions have been detected in time-gated images taken at suitable delays.
Hybrid Langmuir-Blodgett Films Formed by Alternating Layers of Magnetic Polyoxometalate Clusters and Organic Donor Molecules—Towards the Preparation of Multifunctional Molecular Materials (2001)

Alternating monolayers of tetrathiafulvalene and polyoxometalate clusters (see Figure) are assembled here to form hybrid Langmuir–Blodgett (LB) films. The synthetic strategy used is promising for creating multilayer structures comprising two different types of functional units that can impart an interesting combination of properties to the LB film. In the reported example, the high magnetic moment of the polyoxometalate is combined with the ability of the tetrathiafulvalene to delocalize electrons.
Cytotoxic-T-Lymphocyte Human Papillomavirus Type 16 E5 Peptide with CpG-Oligodeoxynucleotide Can Eliminate Tumor Growth in C57BL/6 Mice (2004)

Previously, we identified human papillomavirus type 16 (HPV-16) E5 as a tumor rejection antigen that can induce cytotoxic T lymphocytes (CTLs) to protect against tumor growth (D. W. Liu et al., J. Virol. 74:9083-9089, 2000). In the present study, we further mapped the CTL epitope of E5 protein by analyzing E5-specific CD8(+) gamma interferon-positive (IFN-gamma(+)) double-positive cells in C57BL/6 mice with flow cytometry. The results showed the region spanning amino acids 25 to 33 (VCLLIRPLL) contained the potential D(b)-restricted CTL epitope. Subsequently, to determine whether peptide E5 25-33-based vaccination could induce E5-specific CTL activity, syngeneic animals received E5 25-33 emulsified with either CpG oligodeoxynucleotide (CpG ODN 1826) or Freund's adjuvant, and the growth of the tumors was monitored. The results showed that although both adjuvants induced E5-specific CD8(+) IFN-gamma(+) T cells and eradicated E5-containing tumor growth, CpG ODN was found to stimulate stronger CTL response than Freund's adjuvant. We also compared the immune response of the effector/memory/recall phase induced by E5 25-33 peptide or by E5 protein that was synthesized in vivo by adenovirus-based E5 gene delivery. E5 25-33 peptide plus CpG ODN was shown to be a superior vaccine compared to the adenovirus-based E5 gene. Interestingly, their chronological patterns of immune response were similar, suggesting that E5 25-33 is a major CTL peptide of E5 protein.
The HP AutoRAID hierarchical storage system (1995)

article Free Access Share on The HP AutoRAID hierarchical storage system Authors: J. Wilkes Hewlett-Packard Laboratories, Palo Alto, CA Hewlett-Packard Laboratories, Palo Alto, CAView Profile , R. Golding Hewlett-Packard Laboratories, Palo Alto, CA Hewlett-Packard Laboratories, Palo Alto, CAView Profile , C. Staelin Hewlett-Packard Laboratories, Palo Alto, CA Hewlett-Packard Laboratories, Palo Alto, CAView Profile , T. Sullivan Hewlett-Packard Laboratories, Palo Alto, CA Hewlett-Packard Laboratories, Palo Alto, CAView Profile Authors Info & Claims ACM SIGOPS Operating Systems ReviewVolume 29Issue 5Dec. 3, 1995 pp 96–108https://doi.org/10.1145/224057.224065Published:03 December 1995Publication History 95citation648DownloadsMetricsTotal Citations95Total Downloads648Last 12 Months37Last 6 weeks7 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Relative Quantification of Carboxylic Acid Metabolites by Liquid Chromatography−Mass Spectrometry Using Isotopic Variants of Cholamine (2007)

Labeling reagents that differ only in their isotopic composition offer a powerful approach to achieve relative quantification between samples by ESI-MS. Heavy and light isotopic forms of cholamine, which contain a positively charged quaternary ammonium group, were synthesized and tested as new labeling reagents for the relative quantification of carboxylic acid-containing metabolites, specifically fatty acids. The positive charge on cholamine ensures that the labeled product is also positively charged under all LC-MS conditions, regardless of mobile-phase pH. This leads to high ionization efficiency and correspondingly high detection sensitivity, demonstrated here for the analysis of fatty acids in positive ion mode ESI-MS after reversed-phase separation under acidic conditions. Good accuracy and precision were obtained by mixing heavy- and light-labeled hydrolyzed egg lipid extracts in different known ratios. The relative quantification results for 10 observed fatty acids had an average absolute error of 4.6% and an average coefficient of variation (CV) of 2.6%. The labeling strategy yielded a median CV of 6% when employed for fatty acid analysis of eggs from chickens fed various dietary supplements.
Treatment of Iron Deficiency After Gastric Bypass (2020)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Adaptive separation of independent sources: a deflation approach (2002)

In this paper, we address the adaptive blind source separation of independent sources using higher order statistics. Although this problem was considered in numerous works, none of the existing algorithms is guaranteed to converge to a relevant solution. Here, we propose a new separation scheme whose convergence is proved analytically. It is based on the observation that it is possible to extract one of the source signals by a simple algorithm obtained by extending to the source separation context some of the ideas developed by Shalvi-Weinstein in the framework of blind deconvolution. A low cost deflation procedure allows the extraction of the other source signals by means of the same algorithm. Simulation results illustrate the behaviour of this separation method.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Gene expression and synthesis of natriuretic peptides by cultured human glomerular cells (1999)

Our results suggest differences in the synthesis of natriuretic peptides between glomerular mesangial and epithelial cells.
In Vitro Accessibility of Untreated, Toasted, and Extruded Soybean Meals for Proteases and Carbohydrases (1997)

The in vitro accessibility of the water unextractable solids (WUS) from untreated, toasted, and extruded soybean meals toward different enzyme activities was studied. WUS was incubated with seven commercial enzyme preparations. Two enzyme preparations were selected for further research. Upon addition of Neutrase, the extruded sample yielded considerably more solubilized protein compared with the toasted and untreated soybean meals. Energex solubilized high amounts of neutral sugars after heat treatments compared with the untreated meal. Cell wall polysaccharides solubilized by the enzymes were released as small oligosaccharides and monosaccharides. SDS−PAGE analysis showed that after enzyme addition to the extruded sample, proteins were more rapidly and completely degraded compared with enzyme addition to the toasted and untreated soybean meals. Neutrase degraded both β-conglycinin and glycinin. Energex could only, partly, degrade β-conglycinin. The basic polypeptide from glycinin showed the highest resistance against proteolytic activity. Keywords: Soybean meal; toasting; extrusion; enzymes; protein; cell wall components
Systematic sequencing of theEscherichia coligenome: analysis of the 0 – 2.4 min region (1992)

A contiguous 111,402-nucleotide sequence corresponding to the 0 to 2.4 min region of the E. coli chromosome was determined as a first step to complete structural analysis of the genome. The resulting sequence was used to predict open reading frames and to search for sequence similarity against the PIR protein database. A number of novel genes were found whose predicted protein sequences showed significant homology with known proteins from various organisms, including several clusters of genes similar to those involved in fatty acid metabolism in bacteria (e.g., betT, baiF) and higher organisms, iron transport (sfuA, B, C) in Serratia marcescens, and symbiotic nitrogen fixation or electron transport (fixA, B, C, X) in Azorhizobium caulinodans. In addition, several genes and IS elements that had been mapped but not sequenced (e.g., leuA, B, C, D) were identified. We estimate that about 90 genes are represented in this region of the chromosome with little spacer.
Genetics and Epilepsy: A Model from Critical Path Analysis (1971)

SUMMARY Using a critical path analysis for the aetiology of temporal lobe epilepsy, we suggest that one group of patients have a specific neuropathology, namely mesial temporal sclerosis. The lesion is the product of febrile status epilepticus, which damages Ammon's Horn by convulsive hypoxia. In all cases a specific immaturity in the affected hemisphere is required. In roughly one third of all cases of temporal lobe epilepsy the patient is set up to convulse by a simple gene of transient expression. The protracted duration of the critical seizure is a major determinant. Animal analogues are used to illustrate the hypothesis. RÉSUMÉ 'après une analyse critique de 'étiologie de 'épilepsie du lobe temporal, nous pensons qu'un groupe de sujets présente une neuropathologie spécifique, c'est‐à‐dire une sclérose temporale mesiale. Cette lésion est la conséquence 'un état de mal épileptique fébrile avec lésion de la corne 'Ammone par hypoxie convulsive. Pour que cela se produise il faut aussi dans tous les cas une immaturité spécifique de 'hémisphère atteint. Dans un tiers des cas environ 'épilepsie du lobe temporal, les convulsions sont dûes à un gene simple à expression transitoire. La durée de la crise est un facteur essentiel. Cette hypothèse est aussi étayée par des conditions ex‐périmentales analogues chez 'animal.
Flow cytometry of X and Y chromosome‐bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342 (1987)

A new and improved method of preparing mammalian spermatozoa for high resolution flow cytometric DNA analysis and flow sorting is described. Ejaculated or cryopreserved sperm were briefly sonicated to remove tails and then stained with Hoechst 33342. This simple procedure was found superior to more severe treatments of dimethylsulfoxide washes, fixation in 80% ethanol, and protease digestion of the sperm membranes and tails by papain. Flow cytometric DNA analyses of sperm samples subjected to varying sonication times indicated that X and Y chromosome-bearing sperm populations could be well resolved with as little as 15-sec sonication. In addition, a comparison of sonicated samples stained with four concentrations of bisbenzimide (Hoechst 33342) or 4',6-diamidino-2-phenylindole (DAPI) indicated that 2.5 or 5.0 micrograms/ml of Hoechst was sufficient to resolve the X and Y sperm populations. In order to quantitatively describe the flow cytometric data, several indices (sample quality, orientation and splitting) were developed.
Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies (2005)

Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella. Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as the reservoir for cyclic outbreaks of disease. DTaP vaccines, which are less reactogenic than DTP vaccines, are now in general use in many developed countries, and it is expected that the expansion of their use to adolescents and adults will have a significant impact on reducing pertussis and perhaps decrease the circulation of B. pertussis. Future studies should seek to determine the cause of the unique cough which is associated with Bordetella respiratory infections. It is also hoped that data gathered from molecular Bordetella research will lead to a new generation of DTaP vaccines which provide greater efficacy than is provided by today's vaccines.
Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors (2002)

The location and lineage of cells that give rise to endocrine islets during embryogenesis has not been established nor has the origin or identity of adult islet stem cells. We have employed an inducible Cre-ER(TM)-LoxP system to indelibly mark the progeny of cells expressing either Ngn3 or Pdx1 at different stages of development. The results provide direct evidence that NGN3+ cells are islet progenitors during embryogenesis and in adult mice. In addition, we find that cells expressing Pdx1 give rise to all three types of pancreatic tissue: exocrine, endocrine and duct. Furthermore, exocrine and endocrine cells are derived from Pdx1-expressing progenitors throughout embryogenesis. By contrast, the pancreatic duct arises from PDX1+ progenitors that are set aside around embryonic day 10.5 (E9.5-E11.5). These findings suggest that lineages for exocrine, endocrine islet and duct progenitors are committed at mid-gestation.