gebhart/patrepeval huggingface dataset:
Workshop on In Vitro Neutralization of Chlamydia trachomatis: Summary of Proceedings (1993)

A task force evaluated an in vitro antibody-mediated chlamydial neutralization assay for its utility as a method to assess functional correlates of antibody responses to Chlamydia trachomatis. Two monoclonal antibodies that recognize different major outer membrane protein (MOMP) epitopes for a C. trachomatis serovar B strain exhibit good in vitro neutralizing activity, with a maximum of 90% neutralization. Calculations based on the 50% neutralization point indicated that 100% neutralization could theoretically be achieved when only 10% of the MOMP molecules bound antibody. Monoclonal antibodies that recognized either a heterologous MOMP or the genus-specific chlamydial lipopolysaccharide did not produce neutralizing activity. The standardized assay will be useful to establish if in vitro neutralizing antibody responses are predictive of protective immunity and will aid in defining chlamydial antigens and epitopes that may be attractive vaccine candidates.
Sesquiterpene lactone parthenolide suppresses tumor growth in a xenograft model of renal cell carcinoma by inhibiting the activation of NF-κB (2007)

The transcription factor nuclear factor-kappaB (NF-kappaB) has been shown to be constitutively activated in various human malignancies, including leukemia, lymphoma and a number of solid tumors. NF-kappaB regulates the transcriptional of genes important for tumor invasion, metastasis and chemoresistance. The sesquiterpene lactone parthenolide, an inhibition of NF-kappaB, has been used conventionally to treat migraines and inflammation. In this study, renal cancer cell lines OUR-10 and ACHN were used for in vitro experiments to evaluate growth-inhibitory effects of parthenolide. An OUR-10 xenograft model in nude mice was also used to investigate the in vivo growth-inhibitory effects of parthenolide. Apoptosis in response to treatment of OUR-10 cells with parthenolide was confirmed. Localization of NF-kappaB in response to parthenolide treatment was examined of by immunofluorostaining of OUR-10 cells with antibody against NF-kappaB p65 and by Western blot analysis of OUR-10 cell and tumor nuclear and cytosol fraction. Parthenolide effectively inhibited proliferation of cultured OUR-10 cells and triggered apoptosis in vitro. Subcutaneous injection or oral administration of parthenolide showed significant tumor growth inhibition in the xenograft model via decreased production of interleukin-8 (IL-8) or vascular endothelial growth factor (VEGF). Immunohistochemistry and Western blot analysis showed decreased nuclear localization of NF-kappaB and phosphorylated NF-kappaB protein and subsequently expression of MMP-9, Bcl-xL and Cox-2 in response to parthenolide treatment. These results indicate that parthenolide is a useful in the treatment of renal cell carcinoma and acts via inhibition of NF-kappaB.
Synthesis of sugars by aldolase-catalyzed condensation reactions (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of sugars by aldolase-catalyzed condensation reactionsChi Huey Wong and George M. WhitesidesCite this: J. Org. Chem. 1983, 48, 19, 3199–3205Publication Date (Print):September 1, 1983Publication History Published online1 May 2002Published inissue 1 September 1983https://doi.org/10.1021/jo00167a012Request reuse permissionsArticle Views867Altmetric-Citations115LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (894 KB) Get e-Alertsclose Get e-Alerts
Targeting of a CD8 T Cell Env Epitope Presented by HLA-B*5802 Is Associated with Markers of HIV Disease Progression and Lack of Selection Pressure (2008)

In HIV-infected persons, certain HLA class I alleles are associated with effective control of viremia, while others are associated with rapid disease progression. Among the most divergent clinical outcomes are the relatively good prognosis in HLA-B*5801 expressing persons and poor prognosis with HLA-B*5802. These two alleles differ by only three amino acids in regions involved in HLA-peptide recognition. This study evaluated a cohort of over 1000 persons with chronic HIV clade C virus infection to determine whether clinical outcome differences associated with B*5801 (n = 93) and B*5802 ( n = 259) expression are associated with differences in HIV-1-specific CD8 (+) T cell responses. The overall breadth and magnitude of HIV-1-specific CD8(+) T cell responses were lower in persons expressing B*5802, and epitope presentation by B*5802 contributed significantly less to the overall response as compared to B*5801-restricted CD8 (+) T cells. Moreover, viral load in B*5802-positive persons was higher and CD4 cell counts lower when this allele contributed to the overall CD8 (+) T cell response, which was detected exclusively through a single epitope in Env. In addition, persons heterozygous for B*5802 compared to persons homozygous for other HLA-B alleles had significantly higher viral loads. Viral sequencing revealed strong selection pressure mediated through B*5801-restricted responses but not through B*5802. These data indicate that minor differences in HLA sequence can have a major impact on epitope recognition, and that selective targeting of Env through HLA-B*5802 is at least ineffectual if not actively adverse in the containment of viremia. These results provide experimental evidence that not all epitope-specific responses contribute to immune containment, a better understanding of which is essential to shed light on mechanisms involved in HIV disease progression.
In situ hybridisation. (1990)

In situ hybridisation of mRNA in tissues or cell preparations is a powerful technique for studying gene expression. When combined with cell phenotyping with monoclonal antibodies it gives insights into the cellular basis of disease in vivo. The technique has also been used widely to identify foreign nucleic acids--for example, bacterial or viral, in host cells. The major disadvantages of this approach in the past have been that it was technically demanding, time consuming, and provided qualitative rather than quantitative results. Now, with the use of non-radioactive probes and improved imaging systems, the full potential of this form of molecular analysis is increasingly accessible and should generate rapid advances in many fields.
Progress toward 20% efficiency in Cu(In,Ga)Se2 polycrystalline thin-film solar cells (1999)

This short communication reports on achieving 18·8% total-area conversion efficiency for a ZnO/CdS/Cu(In,Ga)Se2/Mo polycrystalline thin-film solar cell. We also report a 15%-efficient, Cd-free device fabricated via physical vapor deposition methods. The Cd-free cell includes no buffer layer, and it is fabricated by direct deposition of ZnO on the Cu(In,Ga)Se2 thin-film absorber. Both results have been measured at the National Renewable Energy Laboratory under standard reporting conditions (1000 W/m2, 25°C, ASTM E 892 Global). The 18·8% conversion efficiency represents a new record for such devices (Notable Exceptions) and makes the 20% performance level by thin-film polycrystalline materials much closer to reality. We allude to the enhancement in performance of such cells as compared to previous record cells, and we discuss possible and realistic routes to enhance the performance toward the 20% efficiency level. Published in 1999 by John Wiley & Sons, Ltd. This article is a US government work and is in the public domain in the United States.
III-nitride ultraviolet light-emitting diodes with delta doping (2003)

We present the results on the fabrication and characterization of 340 nm UV light-emitting diodes (LEDs) based on InAlGaN quaternary alloys grown by metalorganic chemical vapor deposition. By employing δ doping in the n- and p-type layers, we have demonstrated enhanced LED structural quality and emission efficiency. Combining with our interconnected microdisk LED architecture, the output power of a 300×300 μm2 bare LED chip measured from the sapphire side reached 50 μW under a standard dc operation condition (20 mA) at 4.6 V and 1.6 mW under a pulsed driving current.
Platelet Function in Acute Myocardial Infarction Treated With Direct Angioplasty (1996)

After transient apparent deactivation of circulating platelet, probably caused by sequestration of hyperactive platelets, the level of platelet activation increases in patients with AMI treated by direct PTCA. These findings underscore the need for novel antiplatelet strategies in AMI.
Survivin Enhances Aurora-B Kinase Activity and Localizes Aurora-B in Human Cells (2002)

Survivin, one of the most tumor-specific gene products, has been implicated in both anti-apoptosis and cytokinesis. However, the mechanism by which survivin regulates these two different processes is still elusive. Here, we show that survivin binds to the catalytic domain of Aurora-B. We demonstrate that in the presence of survivin, Aurora-B phosphorylates histone H3 much more efficiently than in the absence of survivin in a cell-free system. Furthermore, we confirm that cells lacking survivin due to survivin antisense oligonucleotide-treatment have lower Aurora-B kinase activity, whereas cells overexpressing survivin have higher Aurora-B kinase activity. We also provide evidence that depletion of survivin by survivin antisense oligonucleotide treatment causes significant reduction of endogenous phosphorylated histone H3 and mislocalization of Aurora-B. These results indicate that survivin stimulates Aurora-B kinase activity and helps correctly target Aurora-B to its substrates during the cell cycle, thus providing a mechanism as to how survivin exerts its function in human cells.
Tumor infiltrating lymphocytes: The regulator of melanoma evolution (Review) (2019)

Melanoma is the most severe type of skin cancer and its incidence has increased in the last decades. In the United States, it is the 6th most common cancer in both men and women. Prognosis for patients with melanoma depends on the stage of the disease at the time of diagnosis and it can be influenced by the immunologic response. Melanoma has been historically considered an immunogenic malignancy. It often contains great amount of immune cells (different subsets of T-cells, dendritic cells, macrophages, neutrophils, mast cells, B lymphocytes), which may reflect a continuous intercommunication between host and tumor. It is not established if tumor-infiltrating lymphocytes (TILs) are induced by tumor cells or by other components of the microenvironment or when they are a host direct immunologic reaction. It has been observed that in many cases, the presence of a dense TIL is associated with good prognosis. The pattern and activation state of the cells which constitute TIL is variable and modulates the clinical outcome. An important step in the understanding of tumor immunobiology is the analysis of the populations and subsets of immune cells that form TIL. Besides its prognostic significance, after approval of cytotoxic T lymphocyte antigen 4, programmed cell death-1 and programmed death-1 ligand antibodies for the treatment of melanoma, the assessment of immune infiltrate composition has become even more captivating, as it could provide new target molecules and new biomarkers for predicting the effect of the treatment and disease outcome in patients treated with immunotherapy. In this review we discuss current state of knowledge in the field of immune cells that infiltrate melanoma, resuming the potential of TIL components to become prognostic markers for natural evolution, for response to drugs or valuable targets for new medication.
A Review of Bloat in Ruminants (1945)

Many recent papers have referred to the literature bearing upon certain aspects, but no one heretofore has reviewed all phases of ruminal physiology and pathology relating to the bloat problem. For the purposes of acquainting workers with all phases of the problem and revealing the gaps in our present knowledge, such a review would seem desirable and is being attempted in the present article. Cattle, sheep, goats, and probably all other ruminants are subject to bloat. To our knowledge no one has attempted to determine experimentally the relative susceptibility of different species to this condition. From a survey in Scotland, McCandlish (121) obtained evidence that dairy cows were more susceptible than beef animals or sheep. Mead, Cole and Regan (125) noted that the feeding of green alfalfa tops caused less serious bloat in sheep than in dairy cows. Since, however, they compared only four cows and four sheep, the difference could easily be explained on the basis of individual susceptibility rather than upon a species difference.
Early Detection of Epidermal Dust-like Particles in Experimentally UV-induced Lesions in Patients with Photosensitivity and Lupus Erythematosus (1998)

Dust-like particles, producing a specific fine-speckled, epidermo-subepidermal direct immunofluorescence staining pattern, have been associated mainly with subacute cutaneous lupus erythematosus (LE). Under experimental conditions the appearance of immunoglobulins along the basement membrane in ultraviolet (UV) light-induced lesions has been reported as a late phenomenon. In this study, photoprovocations with UVA and UVB light were carried out in 16 photosensitive patients with discoid (n = 13), subacute cutaneous (n = 2) or systemic LE (n = 1) and serial biopsies from UV-induced lesions were processed for direct immunofluorescence. A specific, fine-speckled epidermal staining was detected within 7 to 14 days after UV provocation in 7/16 of the patients; in the majority of those patients associated with anti-SSA antibodies adn discoid LE without systemic manifestations of their disease.
Blockade of oncogenic IκB kinase activity in diffuse large B-cell lymphoma by bromodomain and extraterminal domain protein inhibitors (2014)

In the activated B-cell-like (ABC) subtype of diffuse large B-cell lymphoma (DLBCL), NF-κB activity is essential for viability of the malignant cells and is sustained by constitutive activity of IκB kinase (IKK) in the cytoplasm. Here, we report an unexpected role for the bromodomain and extraterminal domain (BET) proteins BRD2 and BRD4 in maintaining oncogenic IKK activity in ABC DLBCL. IKK activity was reduced by small molecules targeting BET proteins as well as by genetic knockdown of BRD2 and BRD4 expression, thereby inhibiting downstream NF-κB-driven transcriptional programs and killing ABC DLBCL cells. Using a high-throughput platform to screen for drug-drug synergy, we observed that the BET inhibitor JQ1 combined favorably with multiple drugs targeting B-cell receptor signaling, one pathway that activates IKK in ABC DLBCL. The BTK kinase inhibitor ibrutinib, which is in clinical development for the treatment of ABC DLBCL, synergized strongly with BET inhibitors in killing ABC DLBCL cells in vitro and in a xenograft mouse model. These findings provide a mechanistic basis for the clinical development of BET protein inhibitors in ABC DLBCL, particularly in combination with other modulators of oncogenic IKK signaling.
The impact of imperfect scheduling on cross-layer rate control in wireless networks (2005)

In this paper, we study cross-layer design for rate control in multihop wireless networks. In our previous work, we have developed an optimal cross-layered rate control scheme that jointly computes both the rate allocation and the stabilizing schedule that controls the resources at the underlying layers. However, the scheduling component in this optimal cross-layered rate control scheme has to solve a complex global optimization problem at each time, and hence is too computationally expensive for online implementation. In this paper, we study how the performance of cross-layer rate control can be impacted if the network can only use an imperfect (and potentially distributed) scheduling component that is easier to implement. We study both the case when the number of users in the system is fixed and the case with dynamic arrivals and departures of the users, and we establish desirable results on the performance bounds of cross-layered rate control with imperfect scheduling. Compared with a layered approach that does not design rate control and scheduling together, our cross-layered approach has provably better performance bounds, and substantially outperforms the layered approach. The insights drawn from our analyses also enable us to design a fully distributed cross-layered rate control and scheduling algorithm for a restrictive interference model.
Quantitative flow measurement with the fast Fourier flow technique. (1988)

The fast Fourier flow method of magnetic resonance (MR) imaging offers a fast and efficient way of measuring quantitative flow for velocities within the range from several millimeters per second to more than 1 m/sec. When fast Fourier flow imaging is used in combination with echocardiographic gating, arterial flow can be determined and velocity versus time profiles can be generated. Because of the high spatial resolution, down to 0.3 mm, detailed examination of flow profiles is possible even in smaller vessels. The method works equally well on a low-field-strength system operating at 0.23 T and on a high-field-strength system with 4.7-T field strength. Since the experiment is very fast, with acquisition times ranging from 4 seconds for a single-section experiment to about 4 minutes for the electrocardiographically gated variant, it can easily be combined with a conventional MR imaging examination.
Block of c-myc expression by antisense oligonucleotides inhibits proliferation of human thyroid carcinoma cell lines. (1996)

Although elevated c-myc expression seems to be related to an unfavorable prognosis of human thyroid neoplasias, the role of c-myc overexpression in the process of thyroid carcinogenesis is still unknown. We analyzed c-myc expression in 7 human thyroid carcinoma cell lines, originating from different histotypes, and in 50 fresh thyroid tumors and found a higher level of c-myc mRNA in all the thyroid carcinoma cell lines and in several fresh thyroid tumors compared with normal thyroid. The highest increases occurred in the most malignant cell lines and in undifferentiated human thyroid carcinomas. The block of c-MYC protein synthesis with myc-specific antisense oligonucleotides reduced the growth rate of the thyroid carcinoma cell lines significantly. Our results indicate that c-myc overexpression plays a critical role in the growth of thyroid cancer cells, which supports the hypothesis that the myc proto-oncogene might be involved in the neoplastic progression of thyroid carcinogenesis.
O2‐05‐01: Immunotherapy targeting Alzheimer's phospho‐tau epitope within the microtubule binding region of tau clears pathological tau and prevents functional decline in a mouse model of tauopathy (2009)

Immunotherapy holds great promise for Alzheimer's disease (AD) and other conformational disorders. Recent studies from our group have shown that immunization with an AD specific phospho-tau immunogen Tau379-408[P-Ser396,404] could alleviate brain levels of aggregated tau and slow the progression of motor deficits or prevent cognitive impairments in two different tangle models (Asuni A. et al. J. Neurosci., 2007, Sigurdsson E.M. et al., ICAD Chicago, 2008). To assess potential epitope specificity and safety of this promising therapeutic effect, we are examining several tau epitopes. Here we assessed the efficacy of targeting a region within the microtubule binding site. Homozygous P301L mice were immunized with Tau260-264[P-Ser262] linked to a tetanus toxin helper T-cell epitope in alum adjuvant (n=8) or with adjuvant only (n=7), starting at 2 months. Mice were tested on various sensorimotor tasks (rotarod, traverse beam, locomotor activity) at 5 and 8 months of age. Antibody titers were determined and at 8 months their brains were processed for tau biochemistry and histology. The vaccine elicited a robust antibody response that was associated with a 64% reduction in PHF-1 tau staining (p=0.02) in the dentate gyrus of the right hemisphere but soluble PHF-1 tau levels were unaltered in the whole left hemisphere compared to controls. Analysis of other brain regions, of insoluble tau and with other tau antibodies is underway. The clearance of tau aggregates was accompanied with functional benefits as the control animals deteriorated in their performance on the rotarod (p=0.05) and the traverse beam (p=0.03) from 5 to 8 months of age, whereas the immunized mice performed equally well at both time points. Various locomotor activity measurements did not differ between the groups except that the 8 month old controls obtained a higher maximum velocity in the open field than treated littermates (p<0.01). Together with our previous results, these findings indicate that immunological targeting of various tau epitopes is a potential therapy for AD. However, a direct comparison of these different immunogens will be needed to assess their relative efficacy. Supported by: NIH grant AG032611, the Alzheimer's Drug Discovery Foundation and the Alzheimer's Association.
Novel covalent-type electrophilic polymerization of 2-(perfluoroalkyl)-2-oxazolines initiated by sulfonates (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel covalent-type electrophilic polymerization of 2-(perfluoroalkyl)-2-oxazolines initiated by sulfonatesMasatoshi Miyamoto, Keigo Aoi, and Takeo SaegusaCite this: Macromolecules 1991, 24, 1, 11–16Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January 1991https://pubs.acs.org/doi/10.1021/ma00001a002https://doi.org/10.1021/ma00001a002research-articleACS PublicationsRequest reuse permissionsArticle Views264Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Vascular Endothelial Grrowth Fartor and Pregnancy lhduced Hypertension (2002)

Vascular endothelial growth factor(VEGF)is a specific vascular growth factor,which play an important role in the angiogenesis.VEGF and its receptors are strongly expressed in human placenta,this is very important to maintain the normal development and function of the placenta.Pregnancy induced hypertension is a serious syndrome which has endothelial cell damage as its key link of pathogenesis.Recently,some researchers have found that the abnormal expression of VEGF is related to the pathogenesis of pregnancy induced hypertension syndrome.