gebhart/patrepeval huggingface dataset:
Liquid-chromatographic measurement of biopterin and neopterin in serum and urine (1990)

We report an improved "high-performance" liquid-chromatographic (HPLC) method for measuring biopterin and neopterin in serum and urine. Specimens are acidified, treated with iodine in 0.2 mol/L trichloroacetic acid, party purified on Bio-Rad MP-50 cation-exchange columns, and analyzed by reversed-phase HPLC with fluorometric detection. The minimal concentration of biopterin detectable is 0.3 micrograms/L in a 50-microL injection. The total CV is less than or equal to 10%. Improvements over other reported methods include the use of a single, simplified sample-preparation step with a Baker-10 SPE System, and a guard column to increase analytical column stability and analyte recovery. The assay is semiautomated to reduce technician time and improve precision. Mean observed values for biopterin and neopterin in sera of normal human adults were 1.64 and 5.52 micrograms/L, respectively. The mean ratio of neopterin to biopterin in acidified adult urine samples was lower than that found in matched nonacidified samples (n = 10). Serum specimens from diagnosed phenylketonuric (PKU) and hyperphenylalaninemic patients were also analyzed for biopterin and neopterin; the findings agreed with reported values for similar patients. One patient, previously identified as an atypical PKU patient, showed serum values of neopterin and biopterin suggestive of a defect in biopterin synthesis.
Pretreatment of Biomass by Aqueous Ammonia for Bioethanol Production (2009)

The methods of pretreatment of lignocellulosic biomass using aqueous ammonia are described. The main effect of ammonia treatment of biomass is delignification without significantly affecting the carbohydrate contents. It is a very effective pretreatment method especially for substrates that have low lignin contents such as agricultural residues and herbaceous feedstock. The ammonia-based pretreatment is well suited for simultaneous saccharification and co-fermentation (SSCF) because the treated biomass retains cellulose as well as hemicellulose. It has been demonstrated that overall ethanol yield above 75% of the theoretical maximum on the basis of total carbohydrate is achievable from corn stover pretreated with aqueous ammonia by way of SSCF. There are two different types of pretreatment methods based on aqueous ammonia: (1) high severity, low contact time process (ammonia recycle percolation; ARP), (2) low severity, high treatment time process (soaking in aqueous ammonia; SAA). Both of these methods are described and discussed for their features and effectiveness.
Urinary Incontinence in Women of Reproductive Age (1996)

We investigated the prevalence and the consequence of urinary incontinence in the Turkish population, representing a composition of European and Asian populations. During a 12-month period, the standard urinary incontinence questionnaire was answered by 1,250 women of reproductive age who were admitted to the outpatient clinic with various gynecologic complaints, except for women whose main complaints were urinary incontinence. These patients were randomly selected by stratification according to the following five age-groups: 18-24, 25-29, 30-34, 35-39, and 40-44. The prevalence of incontinence was measured for each group. Urinary incontinence prevalence was 24.5%; in 6.6% the incontinence occurred at least once daily. The lowest prevalence was found in the younger age-groups (18-29 years) and the highest in 40-44 years of age. Two hundred and sixty-one incontinent women (85%) had never sought medical help. The results indicated that urinary incontinence was common among women of reproductive age and only few sought medical help. We suggest that more public education about women's urinary problems and more attention to this problem by physicians are the mainstays of management.
Methods in enzymology (1955)

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today truly an essential publication for researchers in all fields of life sciences. Key Features * Supplements index volumes 33, 75, 95, 120, 140, 175, 199, 229, 265, 285, and 320 * Subject index * Contributor index
Highly Crystalline and Conductive Nitrogen‐Doped Mesoporous Carbon with Graphitic Walls and Its Electrochemical Performance (2011)

We present a rational and simple methodology to fabricate highly conductive nitrogen-doped ordered mesoporous carbon with a graphitic wall structure by the simple adjustment of the carbonization temperature of mesoporous carbon nitride without the addition of any external nitrogen sources. By simply controlling the heat-treatment temperature, the structural order and intrinsic properties such as surface area, conductivity, and pore volume, and the nitrogen content of ordered graphitic mesoporous carbon can be controlled. Among the materials studied, the sample heat-treated at 1000 °C shows the highest conductivity, which is 32 times higher than that for the samples treated at 800 °C and retains the well-ordered mesoporous structure of the parent mesoporous carbon nitride and a reasonable amount of nitrogen in the graphitic framework. Since these materials exhibit high conductivity with the nitrogen atoms in the graphitic framework, we further demonstrate their use as a support for nanoparticle fabrication without the addition of any external stabilizing or size-controlling agent, as well as the anode electrode catalysts. Highly dispersed platinum nanoparticles with a size similar to that of the pore diameter of the support can be fabricated since the nitrogen atoms and the well-ordered porous structure in the mesoporous graphitic carbon framework act as a stabilizing and size-controlling agent, respectively. Furthermore the Pt-loaded, nitrogen-doped mesoporous graphitic carbon sample with a high conductivity shows much higher anodic electrocatalytic activity than the other materials used in the study.
Multiple sensor integration for indoor surveillance (2005)

Multiple Sensor Indoor Surveillance (MSIS) is a research project at Accenture Technology Labs aimed at exploring a variety of redundant sensors in a networked environment where each sensor is giving noisy information and the goal is to coherently reason about some aspect of the environment. We describe the objectives of the project, the problems it was designed to solve and some recent results. The environment includes 32 web cameras, an infrared badge ID system, a PTZ camera, and a fingerprint reader. We discuss two concrete problems that we have tackled in this project: (1) Visualizing events detected by 32 cameras during 24 hours, and (2) Localizing people using fusion of multiple streams of noisy sensory data with the contextual and domain knowledge that is provided by both the physical constraints imposed by the local environment and by the people that are involved in the surveillance tasks. We use Self-Organizing Maps to approach the first problem and suggest a Bayesian framework for the second one. The experimental data are provided and discussed.
Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease (2009)

High density peptide microarray technologies can be applied in experimental medicine in general and in clinical immunology in particular. Laboratory diagnostics of autoimmune diseases strongly rely on screening human sera for antibodies against known autoantigens. These assays are still difficult to standardize and quantify. Typically, the results are presented as antibody titers within an assay system. Most assays use recombinant or purified autoantigens that are difficult to obtain and require great efforts of quality control. Here we describe a method to obtain patterns of epitope signatures with peptide microarrays from patients suffering from autoimmune diseases in comparison with healthy controls. One of the final aims will be to define subsets of peptides indicative for marker autoantibodies of autoimmune diseases. Finally, informative epitopes can be used for immunopurifying epitope-specific autoantibodies. Eventually, these antibodies can be further characterized on peptide microarrays displaying mutated epitopes obtained by scanning mutagenesis. Any disease or physiological status that affect humoral immune responses such as autoantibodies in oncology or responses to infections or vaccinations can be monitored.
The generalized lapped transform (GLT) for subband coding applications (2002)

This paper proposes a new family of perfect reconstruction (PR) linear phase filter banks called the generalized lapped transform (GLT). The GLT differs from the traditional lapped orthogonal transform (LOT) in that it is nonorthogonal and hence offers more freedom to avoid blocking effects and improve the coding gain. Since the GLT can also be viewed as a generalization of the traditional discrete cosine transform (DCT), fast algorithms for their implementation are also available.
Epstein-Barr virus and other candidate viruses in the pathogenesis of Hodgkin's disease. (1999)

The epidemiologic and clinicopathologic features of Hodgkin's disease (HD) suggest that an infectious agent is involved in the etiology. Over the last 12 years, evidence has accumulated suggesting that Epstein-Barr virus (EBV) is associated with a proportion of cases: EBV genomes are present in Reed-Sternberg (HRS) cells and viral proteins including LMP1, which has oncogenic potential, are expressed. HD has a complex epidemiology and EBV-associated cases are not randomly distributed. Disease occurring in early childhood and older adult age groups is more likely to be EBV-associated than for young adult cases. Paradoxically, there is more evidence supporting an infectious etiology in the latter group of younger patients. Defective EBV genomes and "hit and run" mechanisms involving EBV cannot account for all cases, and the direct involvement of known viral agents, including other lymphotropic herpesviruses, has largely been excluded. Hitherto unknown virus may be responsible for the peak incidence in young adults, which is a feature of HD in developed countries.
Mucosal Addressin Is Required for the Development of Diabetes in Nonobese Diabetic Mice (1998)

Immune responses are best initiated in the environment of lymphoid tissues wherein circulating lymphocytes enter by interacting with endothelial adhesion molecules. In type 1 diabetes, immune responses against pancreatic islets develop, but the environment in which this occurs remains unidentified. To determine whether lymphocyte homing to lymphoid organs is involved in the pathogenesis of diabetes in nonobese diabetic (NOD) mice, we blocked the function of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1), which is a vascular addressin-mediating lymphocyte homing into mucosal lymphoid tissues, in these mice. While ineffective if started later, a blockade started at 3 wk of age reduced the incidence of diabetes from 50% to 9% (p < 0.01). This finding is associated with Peyer's patch atrophy, a marked decrease of naive (CD44(low) CD45RB(high)) T lymphocytes, and a reduction in the relative numbers of memory (CD44(high)) T lymphocytes in the spleen. The potential of these spleen cells to cause diabetes was diminished. Anti-MAdCAM-1 treatment also inhibited both lymphocyte entry into the pancreas and diabetes development in NOD/SCID recipients after the transfer of lymphocytes derived from the mesenteric lymph nodes of young, but not of diabetic, NOD donors. Therefore, MAdCAM-1 may be required during two distinct steps in an early phase of diabetes development: for the entry of naive lymphocytes into the lymphoid tissues in which diabetes-causing lymphocytes are originally primed, and for the subsequent homing of these lymphocytes into the pancreas. The role of MAdCAM-1 as a mucosal vascular addressin suggests that mucosal lymphoid tissues are involved in the initiation of pathologic immune responses in NOD mice.
The interaction of rabbit skeletal muscle troponin-T fragments with troponin-I (1985)

The interactions of troponin-I (Tn-I) with a variety of fragments spanning the length of the troponin-T (Tn-T) polypeptide chain have been reinvestigated at physiological ionic strength by affinity chromatographic, gel filtration, and circular dichroism methodologies. Strong binding was observed with fragment T2 (residues 159-259) mimicking that observed with whole Tn-T and Tn-I. Partial binding was seen with the shorter cyanogen bromide (CB) fragments of Tn-T in the order CB4 (residues 176-230) greater than CB6 (residues 239-259) or CB5 (residues 152-175). No interaction with Tn-I was observed with fragments (CB2, CB3, T1) encompassing residues 1-158 of Tn-T. Based on the present results and the work of others, the binding region for Tn-I includes residues 159-259 and perhaps extends into the highly helical CB2 region (residues 71-151) of Tn-T. No evidence has been obtained by ourselves or others for the interaction of the CB3 region (1-70) with Tn-I. A significant increase (11.6%) in alpha-helical content was observed when an equimolar amount of fragment T2 (residues 159-259) was mixed with Tn-I, a result similar to that seen with whole Tn-T and Tn-I.
Realization of Mg(x=0.15)Zn(1−x=0.85)O-based metal-semiconductor-metal UV detector on quartz and sapphire (2005)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation S. S. Hullavarad, S. Dhar, B. Varughese, I. Takeuchi, T. Venkatesan, R. D. Vispute; Realization of Mg(x=0.15)Zn(1−x=0.85)O-based metal-semiconductor-metal UV detector on quartz and sapphire. J. Vac. Sci. Technol. A 1 July 2005; 23 (4): 982–985. https://doi.org/10.1116/1.1913677 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAVS: Science & Technology of Materials Interfaces and ProcessingJournal of Vacuum Science & Technology A Search Advanced Search |Citation Search
Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptide (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAffinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptideEdward J. Goetzl and Henry MetzgerCite this: Biochemistry 1970, 9, 5, 1267–1278Publication Date (Print):March 1, 1970Publication History Published online1 May 2002Published inissue 1 March 1970https://pubs.acs.org/doi/10.1021/bi00807a031https://doi.org/10.1021/bi00807a031research-articleACS PublicationsRequest reuse permissionsArticle Views76Altmetric-Citations128LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Procoagulant and Anticoagulant Mechanisms in Human Placenta (2003)

The placenta is a highly vascularized organ functioning as the interface between fetal blood, which is confined within the villous blood vessels, and maternal blood, which flows in decidual arteries and washes the intervillous spaces in contact with syncytiotrophoblast (STB) cells. The STB adopts vascular characteristics such as the presence of von Willebrand factor (vWF), CD31 markers, adhesion molecules, and coagulation components. The special structure of the placenta requires efficient mechanisms for fast activation and localized regulation of coagulation. The presence of procoagulant and anticoagulant components on placental vascular endothelial cells (EC) and STB is essential for hemostasis. Activation of coagulation may be a favored process, as suggested by elevated fibrin depositions documented in some pathologic states. Increased localized procoagulant components such as tissue factor (TF) and plasminogen activator inhibitors (PAI-1, PAI-2), are associated with some pregnancy complications. Several anticoagulants regulate placental coagulation: tissue factor pathway inhibitor (TFPI) is primarily produced in EC; TFPI-2, a variant of TFPI, has been purified from the placenta and was identified in the STB lining the villi; thrombomodulin, a membrane glycoprotein that activates protein C, is localized in EC and apical membranes of STB; annexin V, an anticoagulant that binds to negative membrane phospholipids, is abundant on normal placental STB, whereas reduced STB annexin V was associated with the presence of antiphospholipid antibodies. The placenta is a putative source of coagulation components. However, the interplay between local procoagulant and anticoagulant mechanisms and their association with pregnancy complications need to be assessed.
Using the Velvet de novo Assembler for Short‐Read Sequencing Technologies (2010)

The Velvet de novo assembler was designed to build contigs and eventually scaffolds from short-read sequencing data. This protocol describes how to use Velvet, interpret its output, and tune its parameters for optimal results. It also covers practical issues such as configuration, using the VelvetOptimiser routine, and processing colorspace data.
Surface modification of titanium with hydroxyapatite-heparin-BMP-2 enhances the efficacy of bone formation and osseointegrationin vitroandin vivo (2014)

Surface-modified titanium (Ti) samples with hydroxyapatite (HAp) and heparin (Hep)-bone morphogenetic protein-2 (BMP-2) complex (Ti/HAp/Hep/BMP-2) were prepared, and their efficacies on the enhancements of bone formation and osseointegration in vitro and in vivo were examined as compared to Ti/HAp and Ti/Hep/BMP-2. The modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and contact angle goniometry. In vitro studies revealed that MG-63 human osteosarcoma cell lines grown on Ti/HAp/Hep/BMP-2 increased the amounts of alkaline phosphatase (ALP) activity, calcium deposition and the levels of OCN mRNA gene expression as compared to those grown on Ti/HAp, Ti/Hep/BMP-2 or pristine Ti. Moreover, Ti/HAp/Hep/BMP-2 exhibited higher bone volume (BV), bone volume/tissue volume (BV/TV), removal torque value and bone-implant contact (BIC) than Ti/HAp, Ti/Hep/BMP-2 or pristine Ti in vivo. Histological evaluations showed that many desirable features of bone remodelling existed at the interface between Ti/HAp/Hep/BMP-2 and the host bone. Consequently, Ti/HAp/Hep/BMP-2 may have potential for clinical use as dental or orthopaedic implants.
Three-dimensional real-timein vivomagnetic particle imaging (2009)

Magnetic particle imaging (MPI) is a new tomographic imaging method potentially capable of rapid 3D dynamic imaging of magnetic tracer materials. Until now, only dynamic 2D phantom experiments with high tracer concentrations have been demonstrated. In this letter, first in vivo 3D real-time MPI scans are presented revealing details of a beating mouse heart using a clinically approved concentration of a commercially available MRI contrast agent. A temporal resolution of 21.5 ms is achieved at a 3D field of view of 20.4 x 12 x 16.8 mm(3) with a spatial resolution sufficient to resolve all heart chambers. With these abilities, MPI has taken a huge step toward medical application.
Predicting Drug-Likeness: Why and How ? (2002)

There exists a huge attrition rate of molecules in clinical trials. It was expected that high-throughput screening and combinatorial chemistry would make the task of producing drugs easier. However, the efforts of the past decade have not been an unvarnished success. As a result, a lot of experimental and computational efforts are currently being directed at determining the basic requirements for a molecule to become a drug. Here we will review the physiological, structural, and other requirements for obtaining a molecule that will be successful in the clinic. Following this we will provide a description, analysis, and commentary on the computational efforts in this direction. We will focus both on the traditional computational chemistry perspective of starting from the structure of the molecule as well as the traditional computational pharmaceutical scientist's perspective of physiologically based simulations. We end with a few comments about the future and some ideas on re-organizing the pharmaceutical enterprise.
Articular Cartilage Engineering with Hyalograft?? C (2005)

Therapeutic study, Level III-2 (retrospective cohort study). See the Guidelines for Authors for a complete description of levels of evidence.
Epigenetic Inactivation of the Groucho Homologue Gene TLE1 in Hematologic Malignancies (2008)

An undifferentiated status and the epigenetic inactivation of tumor-suppressor genes are hallmarks of transformed cells. Promoter CpG island hypermethylation of differentiating genes, however, has rarely been reported. The Groucho homologue Transducin-like Enhancer of Split 1 (TLE1) is a multitasked transcriptional corepressor that acts through the acute myelogenous leukemia 1, Wnt, and Notch signaling pathways. We have found that TLE1 undergoes promoter CpG island hypermethylation-associated inactivation in hematologic malignancies, such as diffuse large B-cell lymphoma and AML. We also observed a mutual exclusivity of the epigenetic alteration of TLE1 and the cytogenetic alteration of AML1. TLE1 reintroduction in hypermethylated leukemia/lymphoma cells causes growth inhibition in colony assays and nude mice, whereas TLE1-short hairpin RNA depletion in unmethylated cells enhances tumor growth. We also show that these effects are mediated by TLE1 transcriptional repressor activity on its target genes, such as Cyclin D1, Colony-Stimulating Factor 1 receptor, and Hairy/Enhancer of Split 1. These data suggest that TLE1 epigenetic inactivation contributes to the development of hematologic malignancies by disrupting critical differentiation and growth-suppressing pathways.
Direct Electrodeposition of Tetrahexahedral Pd Nanocrystals with High-Index Facets and High Catalytic Activity for Ethanol Electrooxidation (2010)

Tetrahexahedral Pd nanocrystals (THH Pd NCs) with {730} high-index facets were directly produced on a glassy carbon substrate in a dilute PdCl(2) solution by a newly developed programmed electrodeposition method. The THH Pd NCs, thanks to their high density of surface atomic steps, exhibit 4-6 times higher catalytic activity than commercial Pd black catalyst toward ethanol electrooxidation in alkaline solutions. This straightforward method provides a promising route to facile preparation of high-index-faceted metal nanocatalysts with high catalytic activity.
Acute safety, efficacy, and advantages of a novel cryoballoon ablation system for pulmonary vein isolation in patients with paroxysmal atrial fibrillation: initial clinical experience (2021)

In this first human experience, the novel cryoballoon platform was safe, efficacious, and demonstrated a high proportion of successful single ablation isolation.