gebhart/patrepeval huggingface dataset:
Delayed Onset of Inflammation in Protease-Activated Receptor-2-Deficient Mice (2000)

Endothelial surface expression of P-selectin and subsequent leukocyte rolling in venules can be induced by mast cell-derived histamine and binding of thrombin to protease-activated receptor-1 (PAR1). We hypothesized that activation of endothelial PAR2 by mast cell tryptase or other proteases also contributes to inflammatory responses. Leukocyte rolling flux and rolling velocity were assessed by intravital microscopy of the cremaster muscles of wild-type mice following perivenular micropipette injections of a control (LSIGRL) or PAR2-activating (SLIGRL) oligopeptide. Injection of SLIGRL increased mean rolling leukocyte flux fraction from 34 +/- 11 to 71 +/- 24% (p < 0.05) and decreased mean rolling velocity from 63 +/- 29 to 32 +/- 2 micrometer/s (p < 0.05). No significant changes occurred with control peptide injection. To further evaluate the role of PAR2 in inflammatory responses, PAR2-deficient mice were generated by gene targeting and homologous recombination. Perivenular injections of SLIGRL resulted in only a small increase in rolling leukocyte flux fraction (from 21 +/- 8 to 30 +/- 2%) and no change in rolling velocity. Leukocyte rolling after surgical trauma was assessed in 9 PAR2-deficient and 12 wild-type mice. Early (0-15 min) after surgical trauma, the mean leukocyte rolling flux fraction was lower (10 +/- 3 vs 30 +/- 6%, p < 0.05) and mean rolling velocity was higher (67 +/- 46 vs 52 +/- 36 micrometer/s, p < 0.01) in PAR2-deficient compared with control mice. The defect in leukocyte rolling in PAR2-deficient mice did not persist past 30 min following surgical trauma. These results indicate that activation of PAR2 produces microvascular inflammation by rapid induction of P-selectin-mediated leukocyte rolling. In the absence of PAR2, the onset of inflammation is delayed.
Serotonin modulates the oxidative burst of human phagocytes via various mechanisms (2007)

Serotonin, the major secretory product of activated platelets, has been widely reported as regulating various constituents of the immune system and immune functions. This modulation is complex and the data available are rather controversial. The aim of the present study was to clarify the mechanisms of serotonin action on human phagocytes. The effect of serotonin in a concentration range of 10(-7) M-10(-3) M on various parameters of oxidative burst of phagocytes was studied using various luminol-enhanced chemiluminescence methods. Serotonin inhibited the chemiluminescence response of the cells in a dose dependent manner. The effect of serotonin on the activity of myeloperoxidase was studied in further experiments. In this case, serotonin again exerted a dose dependent inhibition of the myeloperoxidase activity. The hypothesis that the inhibitory activity of serotonin might be also receptor mediated was evaluated using various serotonin receptor agonists and antagonists. None of the agonists studied exerted any direct antioxidative properties. Only (+/-)-DOI hydrochloride, a selective 5-HTR(2) agonist, exerted similar effects on phagocytic cells as serotonin. It can be concluded that serotonin could affect the oxidative burst of phagocytes. Responsibility for its inhibitory effects lies with both the decrease in the generation of reactive oxygen species (due to the inhibition of myeloperoxidase activity) and with direct scavenging of reactive oxygen species. The effect of serotonin on phagocytes is also partially mediated by 5-HTR(2) receptor.
Synthesis of 2-(4-Fluorophenyl)-4-isopropyl-3-quinolinecarbaldehyde: A New Route to 2,3,4-Substituted Quinolines (1991)

A new method for the preparation of 2,3,4-substituted quinolines has been developed and its application towards the synthesis of 2-(4-fluorophenyl)-4-isopropyl-3-quinolinecarbaldehyde, as well as other quinolines, is described.
Preoperative serum CA‐125 levels and risk of suboptimal cytoreduction in ovarian cancer: A meta‐analysis (2009)

The current analysis indicates that CA-125 is a strong risk factor of suboptimal cytoreduction and it may be applied in preoperative counseling and treatment planning. However, it also shows that CA-125 lacks the ability to predict optimal cytoreduction accurately.
Morphogenesis of Self-Assembled Nanocrystalline Materials of Barium Carbonate and Silica (2009)

The precipitation of barium or strontium carbonates in alkaline silica-rich environments leads to crystalline aggregates that have been named silica/carbonate biomorphs because their morphology resembles that of primitive organisms. These aggregates are self-assembled materials of purely inorganic origin, with an amorphous phase of silica intimately intertwined with a carbonate nanocrystalline phase. We propose a mechanism that explains all the morphologies described for biomorphs. Chemically coupled coprecipitation of carbonate and silica leads to fibrillation of the growing front and to laminar structures that experience curling at their growing rim. These curls propagate in a surflike way along the rim of the laminae. We show that all observed morphologies with smoothly varying positive or negative Gaussian curvatures can be explained by the combined growth of counterpropagating curls and growing laminae.
Preparation and Characterization of Crosslinked and Polymerized Hemoglobin Solutions (1992)

In 1982 we synthesized 2-Nor-2-formylpyridoxal 5'-phosphate (NFPLP) and subsequently showed that coupling of the beta chains of hemoglobin (Hb) by this organic phosphate compound according to Benesch et al. (1) lowers the oxygen affinity and prolongs the retention time in the circulation of rats and rabbits with a factor 3 by prevention of excretion via the kidneys. Optimal conditions for the purification of HbNFPLP either by ion-exchange chromatography or by heat treatment were established with recoveries of 70% and 85%, respectively. By extrapolation from the data in rats and rabbits a half life of about 8 hours can be expected in the circulation of humans. However, under some conditions a further prolongation is required. The aim of further modification of HbNFPLP was to achieve a retention time of about 24 hours. Polymerization with glutaraldehyde to polyHbNFPLP resulted in a mixture of polymers of different size. We determined the optimal degree of polymerization with respect to the effects on vascular retention time, oncotic activity, viscosity and oxygen affinity. Depending on the degree of polymerization we found in rats a 5- to 7- fold increase in vascular half-life compared to native Hb. The change in oxygen affinity was found to be independent of the polymer size (P50 = 18-22 mmHg). A limiting factor for polymerization is the increase in viscosity, which was dramatic when large polymers (greater than 300 kD) were present in the preparation.(ABSTRACT TRUNCATED AT 250 WORDS)
Regulation of Pancreatic Juxtaductal Endocrine Cell Formation by FoxO1 (2009)

An understanding of the mechanisms that govern pancreatic endocrine cell ontogeny may offer strategies for their somatic replacement in diabetic patients. During embryogenesis, transcription factor FoxO1 is expressed in pancreatic progenitor cells. Subsequently, it becomes restricted to beta cells and to a rare population of insulin-negative juxtaductal cells (FoxO1+ Ins(-)). It is unclear whether FoxO1+ Ins(-) cells give rise to endocrine cells. To address this question, we first evaluated FoxO1's role in pancreas development using gain- and loss-of-function alleles in mice. Premature FoxO1 activation in pancreatic progenitors promoted alpha-cell formation but curtailed exocrine development. Conversely, FoxO1 ablation in pancreatic progenitor cells, but not in committed endocrine progenitors or terminally differentiated beta cells, selectively increased juxtaductal beta cells. As these data indicate an involvement of FoxO1 in pancreatic lineage determination, FoxO1+ Ins(-) cells were clonally isolated and assayed for their capacity to undergo endocrine differentiation. Upon FoxO1 activation, FoxO1+ Ins(-) cultures converted into glucagon-producing cells. We conclude that FoxO1+ Ins(-) juxtaductal cells represent a hitherto-unrecognized pancreatic cell population with in vitro capability of endocrine differentiation.
Construction and testing of a computer-based intraoral laser scanner for determining tooth positions (2000)

An optical set-up for intraoral data acquisition based on the principle of laser triangulation was developed. The system consists of a pig-tailed laser with line generating optics, a stepping motor driven positioning stage, a commercial CCD (charge coupled device) camera system with frame grabber interface, a control personal computer and a mirror system compensating for the fact that there is no possibility of watching an object directly in the mouth under a certain angle except from a facial position during intraoral scanning. Due to the size of the prototype measurements were still restricted to plaster casts. In order to evaluate its accuracy, the measurements were compared with those taken with a commercial laser scanner and a coordinate measurement table. The accuracy of the prototype scanner was determined to be DeltaXYZ=0.04 mm using gauge blocks of given dimensions and proved to range between the commercial laser scanner and the coordinate measurement table (i.e., it was slightly better than that of the commercial scanner). Applications in orthodontics were demonstrated by scanning plaster casts and measuring distances on reconstructed surfaces. The measured distances showed a maximum deviation of about +/-0.2 mm compared with the data of the coordinate measurement table, which served as a reference. In addition, reconstruction of three-dimensional tooth movements was performed on the scan data. The translational and rotational parameters gained from the superimposition of scanned point clouds and describing tooth movement were also in good accordance with the reference. The achieved accuracy proved to be sufficient for further development which should include a reduction in size and the use of more precise device components.
In Vitro Fertilization Rates after Varicocele Repair (1982)

Causal relationship of varicocele to infertility is controversial. Studies of hormonal milieu, semen and testicular tissue yield data of debatable significance. We herein assay the fertilizing ability of men with varicocele and monitor changes consequent to surgical correction of varicocele. Zona-free hamster ova were used to determine fertilizing ability. A fertilization rate of less than 10 per cent correlates with infertility. Between May 1978 and November 1979, 75 men with varicocele presenting for fertility evaluation and 23 men with painful varicocele were tested. Of the patients infertile clinically and by in vitro fertilization assay 42 elected ligation of the internal spermatic vein(s). Average fertilization rate of the painful varicocele group was 20.5 per cent and for the infertile group it was 1.2 per cent. Average fertilization rates postoperatively in the infertile group were 2.9 per cent (3 months), 7.7 per cent (6 months), 2.8 per cent (9 months), 7.8 per cent (12 months), 17.5 per cent (15 months) and 8.0 per cent (18 months). Pregnancies occurred only in partners of men whose fertilization rates were between 2.9 and 8.3 per cent preoperatively and who improved to greater than 10 per cent postoperatively. The in vitro fertilization assay may help select those individuals with varicocele and infertility most likely to benefit from varicocele repair.
PEDAGOGY THAT WORKS (2010)

RETURN TO ISSUEPREVNewsNEXTPEDAGOGY THAT WORKSC&EN examines TWO TRENDS: Funding agencies push for assessment of education projects, and chemistry professors maintain multiple research groups and labs worldwideMAUREEN ROUHICite this: Chem. Eng. News 2010, 88, 37, 47Publication Date (Print):September 13, 2010Publication History Published online27 March 2011Published inissue 13 September 2010https://pubs.acs.org/doi/10.1021/cen-v088n037.p047https://doi.org/10.1021/cen-v088n037.p047newsACS PublicationsCopyright © 2010 AMERICAN CHEMICAL SOCIETYArticle Views13Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Students Get e-Alerts
Fiber/Proppant Mixtures Control Proppant Flowback in South Texas (1995)

Abstract Backproduction of proppant from hydraulic fractures (proppant flowback) is a continuing operational problem in the oil and gas industry. Up to 20% of the proppant can be flowed back after the treatment.1 Curable resin-coated proppants are used to control proppant production, but are known to chemically interact with fracturing fluids and may be prone to several failure mechanisms.2 Curable resin-coated proppants also require either well shut-in or the use of activators at low temperatures. A new method to control proppant flowback relies on fibers mixed with the proppant to stabilize the proppant pack. The main advantage of this patented3 technology is that it is physical rather than chemical. Therefore, proppant flowback is controlled without specific shut-in time, temperature, or pressure constraints. This paper presents flowback results from fractures of dry gas wells (&amp;lt;l millidarcy permeability) where fí-ber/proppant mixtures were used to control proppant flow-back (11 cases). Fluid flowback rate, gas rate and proppant production were monitored during the cleanup period. These wells are compared to wells where either curable resin- coated proppants or no flowback control were used (15 cases). The fiber/proppant mixtures controlled flowback of proppant for both sand and ceramic proppants when used with all the proppant or in only the last part of proppant (tail-in). Flowback could begin right after the fracturing equipment was rigged down (15 to 30 minutes). Cleanup fluid flow rates were up to ten times higher than previously obtainable with curable resin-coated proppants and less proppant was flowed back. Faster flowback rates also resulted in earlier onset of gas production and reduced flowback time. Fibers allow greater latitude in flowback rate than curable resincoated proppants without the need for shut-in time.
Cerebral Mast Cells Mediate Blood-Brain Barrier Disruption in Acute Experimental Ischemic Stroke Through Perivascular Gelatinase Activation (2011)

Our data demonstrate that cerebral MCs participate in regulation of acute microvascular gelatinase activation and consequent blood-brain barrier disruption following transient cerebral ischemia.
Diabetes Mellitus Enhances Vascular Matrix Metalloproteinase Activity (2001)

Diabetes mellitus (DM) is a primary risk factor for cardiovascular disease. Although recent studies have demonstrated an important role for extracellular matrix metalloproteinases (MMPs) in atherosclerosis, little is known about the effects of hyperglycemia on MMP regulation in vascular cells. Gelatin zymography and Western blot analysis revealed that the activity and expression of 92-kDa (MMP-9) gelatinase, but not of 72 kDa (MMP-2) gelatinase, were significantly increased in vascular tissue and plasma of two distinct rodent models of DM. Bovine aortic endothelial cells (BAECs) grown in culture did not express MMP-9 constitutively; however, chronic (2-week) incubation with high glucose medium induced MMP-9 promoter activity, mRNA and protein expression, and gelatinase activity in BAECs. On the other hand, high glucose culture did not change MMP-9 activity from vascular smooth muscle cells or macrophages. Electron paramagnetic resonance studies indicate that BAECs chronically grown in high glucose conditions produce 70% more ROS than do control cells. Enhanced MMP-9 activity was significantly reduced by treatment with the antioxidants polyethylene glycol-superoxide dismutase and N-acetyl-L-cysteine but not by inhibitors of protein kinase C. In conclusion, vascular MMP-9 activity is increased in DM, in part because of enhanced elaboration from vascular endothelial cells, and oxidative stress plays an important role. This novel mechanism of redox-sensitive MMP-9 expression by hyperglycemia may provide a rationale for antioxidant therapy to modulate diabetic vascular complications.
Autonomously replicating sequences in Saccharomyces cerevisiae. (1980)

A method is presented for isolating DNA segments capable of autonomous replication from Saccharomyces cerevisiae chromosomal DNA based on the differential transforming ability of autonomously replicating plasmids and nonreplicating plasmids. DNA plasmids that are capable of self-replication in yeast transform yeast spheroplasts at about 1000-fold higher frequency than nonreplicating plasmids. We have cloned from total yeast DNA a number of DNA segments that permit the pBR322 plasmid carrying the yeast LEU2 gene to replicate autonomously. These plasmid clones are characterized by their ability to transform Leu- spheroplasts to Leu+ at a high frequency and their ability to replicate autonomously. Analysis of the insert DNAs carried in some of these self-replicating plasmids divides them into two categories: those that are unique in the yeast genome and those that are repetitive.
DIAGNOSIS OF MALARIA BY MAGNETIC DEPOSITION MICROSCOPY (2006)

Although malaria contributes to a significant public health burden, malaria diagnosis relies heavily on either non-specific clinical symptoms or blood smear microscopy methods developed in the 1930s. These approaches severely misrepresent the number of infected individuals and the reservoir of parasites in malaria-endemic communities and undermine efforts to control disease. Limitations of conventional microscopy-based diagnosis center on time required to examine slides, time required to attain expertise sufficient to diagnose infection accurately, and attrition from the limited number of existing malaria microscopy experts. Earlier studies described magnetic properties of Plasmodium falciparum but did not refine methods to diagnosis infection by all four human malaria parasite species. Here, following specific technical procedures, we show that it is possible to concentrate all four human malaria parasite species, at least 40-fold, on microscope slides using very inexpensive magnets through an approach termed magnetic deposition microscopy. This approach delivered greater sensitivity than a thick smear preparation while maintaining the clarity of a thin smear to simplify species-specific diagnosis. Because the magnetic force necessary to concentrate parasites on the slide is focused at a precise position relative to the magnet surface, it is possible to examine a specific region of the slide for parasitized cells and avoid the time-consuming process of scanning the entire slide surface. These results provide insight regarding new strategies for performing malaria blood smear microscopy.
Evolution of human immunodeficiency virus type 2 coreceptor usage, autologous neutralization, envelope sequence and glycosylation (2005)

To investigate why human immunodeficiency virus type 2 (HIV-2) is less virulent than HIV-1, the evolution of coreceptor usage, autologous neutralization, envelope sequence and glycosylation was studied in sequentially obtained virus isolates and sera from four HIV-2-infected individuals. Neutralization of primary HIV-2 isolates was tested by a cell line-based assay and IgG purified from patients' sera. Significant autologous neutralization was observed for the majority (39 of 54) of the HIV-2 serum-virus combinations tested, indicating that neutralization escape is rare in HIV-2 infection. Furthermore, sera from 18 HIV-2 patients displayed extensive heterologous cross-neutralization when tested against a panel of six primary HIV-2 isolates. This indicates that HIV-2 is intrinsically more sensitive to antibody neutralization than HIV-1. In line with earlier reports, HIV-2 isolates could use several alternative receptors in addition to the major coreceptors CCR5 and CXCR4. Intrapatient evolution from CCR5 use to CXCR4 use was documented for the first time. Furthermore, CXCR4 use was linked to the immunological status of the patients. Thus, all CXCR4-using isolates, except one, were obtained from patients with CD4 counts below 200 cells microl(-1). Sequence analysis revealed an association between coreceptor usage and charge of the V3 loop of the HIV-2 envelope, as well as an association between the rate of disease progression and the glycosylation pattern of the envelope protein. Furthermore, HIV-2 isolates had fewer glycosylation sites in the V3 domain than HIV-1 (two to three versus four to five). It is proposed here that HIV-2 has a more open and accessible V3 domain than HIV-1, due to differences in glycan packing, and that this may explain its broader coreceptor usage and greater sensitivity to neutralizing antibodies.
Rapid temperature tuning of a 14-μm diode laser with application to high-pressure H_2O absorption spectroscopy (2001)

Enhanced wavelength tuning of a distributed-feedback InGaAsP diode laser is demonstrated by use of rapid temperature cycling. The laser-active region is cycled from -10 to +50 degrees C (scanning the output from 1399 to 1403 nm) at kilohertz rates by pulsed heating with an auxiliary 532-nm laser. Such 4-nm scans represent a ten-fold increase in the wavelength-scanning range offered by standard current-tuning techniques and thus extend the capabilities of scan-wavelength sensors and systems. As an example application, we demonstrate absorption spectroscopy of H(2)O vapor at a pressure of 10 atm.
CARBON NANOTUBES AND NANOTUBE-BASED NANO DEVICES (1998)

Carbon nanotubes exhibit unusual electronic and mechanical properties which vary with subtle changes in microstructure, applied electromagnetic field and mechanical deformations, and introduction of topological defects. These novel properties offer unprecedent opportunities to study fundamental physics, fabricate advanced composition materials, and construct quantum devices at nanometer scales.
Monitoring the Performance of a Residential Central Air Conditioner under Degraded Conditions on a Test Bench (1992)

This report presents the measured degradation in performance of a residential air conditioning&#13;\nsystem operating under degraded conditions. Experiments were conducted using a R-22 threeton&#13;\nsplit-type cooling system with a short-tube orifice expansion device. Results are presented&#13;\nhere for a series of tests in which the various commonly occurring degraded conditions were&#13;\nsimulated on a test bench.&#13;\nAt present, very little information is available which quantifies the performance of a residential&#13;\ncooling system operating under degraded conditions. Degraded performance measurements can&#13;\nprovide information which could help electric utilities evaluate the potential impact of systemwide&#13;\nmaintenance programs. This report also discuss the development of a diagnostic procedure&#13;\nbased on measurement of refrigerant and air side temperatures.