gebhart/patrepeval huggingface dataset:
The role of CD11c+ hepatic dendritic cells in immue tolerance to hepatocellular carcinoma (41.48) (2009)

Abstract Immune tolerance is a major mechanism involved in the development of hepatocellular carcinoma (HCC). We have previously showed, in a mouse model of spontaneous HCC, that T cell response can be induced in mice with small HCC, but not in mice with large HCC, indicating that immune tolerance occurs when tumors grow to a certain size. The mechanisms responsible for this phenomenon were not clear. In this study, we compared freshly isolated liver dendritic cell (DC) subsets in mice with no tumor, small HCC, and large HCC. We found that total numbers of CD11c+ hepatic DC were comparable in these 3 groups. However, CD11cint B220+ subset of ’plasmacytoid’ DC (pDC) significantly decreased in both small and large HCC-bearing mice. Importantly, CD11chi B220- subset of mature DC significantly increased in mice with small HCC. In contrast, CD11chi B220- subset of mature DC remains same in mice with large HCC, the CD11cint B220- subset of immature DC increased instead in these mice. The surface phenotype of each subset of DCs were examined and demonstrated higher level of CD86 and CD40 expression by CD11chi subset of mature DC comparing with CD11cint subsets. These findings indicate that modulating subset distribution of liver DC, such as inhibiting the expansion of CD11chi B220- subset of mature DC, may play a role in immune tolerance to HCC.
Stabilization of the Restriction Enzyme EcoRI Dried with Trehalose and Other Selected Glass-Forming Solutes (1997)

The stabilization of the restriction enzyme EcoRI by its incorporation into aqueous glass-forming carbohydrate or polymer solutions, followed by vacuum-drying to low moisture, has been studied. Glass-forming solutes included trehalose, sucrose, lactose, maltose, raffinose, maltodextrin DE 10, and poly(vinylpyrrolidone) (molecular weight 40,000, PVP). Among the solutes examined, trehalose and sucrose protected the enzyme most effectively during storage at 37 and 45 degrees C. The restriction enzyme dried with trehalose or sucrose maintained its activity without detectable loss for at least 20 days at 37 degrees C and 12 days at 45 degrees C. In contrast, the activity of the enzyme dried with maltodextrin or PVP was reduced during vacuum desiccation and also it decreased remarkably during storage at the same temperatures. Stored (37/45 degrees C) vacuum-dried trehalose and sucrose systems were either a dense paste or a very viscous syrup, and this indicated that they were not glassy. Moreover, no relationship was found between the glass transition temperatures (Tg) of the pure added solute and enzyme protection during storage, since, e.g., sucrose which has significantly lower Tg values protected the enzyme much better than either maltose, lactose, maltodextrin, or PVP. The trisaccharide raffinose offered good protection of enzyme activity, and its role as a novel excipient matrix for labile enzyme stabilization deserves further investigation. The stability of enzyme EcoRI was rapidly lost when the vacuum-dried trehalose and sucrose systems were humidified to 58% relative humidity and stored at 45 degrees C, and this was attributed to disaccharide crystallization.
REGULATION OF IMMUNE RESPONSES THROUGH INHIBITORY RECEPTORS (1999)

Major histocompatibility complex class I-specific inhibitory receptors on natural killer cells prevent the lysis of healthy autologous cells. The outcome of this negative signal is not anergy or apoptosis of natural killer cells but a transient abortion of activation signals. The natural killer inhibitory receptors fulfill this function by recruiting the tyrosine phosphatase SHP-1 through a cytoplasmic immunoreceptor tyrosine-based inhibition motif. This immunoreceptor tyrosine-based inhibition motif has become the hallmark of a growing family of receptors with inhibitory potential, which are expressed in various cell types such as monocytes, macrophages, dendritic cells, leukocytes, and mast cells. Most of the natural killer inhibitory receptors and two members of a monocyte inhibitory-receptor family bind major histocompatibility complex class I molecules. Ligands for many of the other receptors have yet to be identified. The inhibitory-receptor superfamily appears to regulate many types of immune responses by blocking cellular activation signals.
DISEASE OF THE HEART IN THE WORKS OF HIPPOCRATES (1962)

He who would make accurate forecasts as to those who will recover, and those who will die, and whether the disease will last a greater or less number of days, must understand all the symptoms thoroughly and be able to appreciate them, estimating their powers when they are compared with one another, as I have set forth. Hippocrates, Prognostic XXV (Jones, 1923-31)
Intracellular Retention of Newly Synthesized Insulin in Yeast Is Caused by Endoproteolytic Processing in the Golgi Complex (2001)

An insulin-containing fusion protein (ICFP, encoding the yeast prepro-alpha factor leader peptide fused via a lysine-arginine cleavage site to a single chain insulin) has been expressed in Saccharomyces cerevisiae where it is inefficiently secreted. Single gene disruptions have been identified that cause enhanced immunoreactive insulin secretion (eis). Five out of six eis mutants prove to be vacuolar protein sorting (vps)8, vps35, vps13, vps4, and vps36, which affect Golgi<-->endosome trafficking. Indeed, in wild-type yeast insulin is ultimately delivered to the vacuole, whereas vps mutants secrete primarily unprocessed ICFP. Disruption of KEX2, which blocks intracellular processing to insulin, quantitatively reroutes ICFP to the cell surface, whereas loss of the Vps10p sorting receptor is without effect. Secretion of unprocessed ICFP is not based on a dominant secretion signal in the alpha-leader peptide. Although insulin sorting mediated by Kex2p is saturable, Kex2p functions not as a sorting receptor but as a protease: replacement of Kex2p by truncated secretory Kex2p (which travels from Golgi to cell surface) still causes endoproteolytic processing and intracellular insulin retention. Endoproteolysis promotes a change in insulin's biophysical properties. B5His residues normally participate in multimeric insulin packing; a point mutation at this position permits ICFP processing but causes the majority of processed insulin to be secreted. The data argue that multimeric assembly consequent to endoproteolytic maturation regulates insulin sorting in the secretory pathway.
Proactive recovery in a Byzantine-fault-tolerant system (2000)

This paper describes an asynchronous state-machine replication system that tolerates Byzantine faults, which can be caused by malicious attacks or software errors. Our system is the first to recover Byzantine-faulty replicas proactively and it performs well because it uses symmetric rather than public-key cryptography for authentication. The recovery mechanism allows us to tolerate any number of faults over the lifetime of the system provided fewer than 1/3 of the replicas become faulty within a window of vulnerability that is small under normal conditions. The window may increase under a denial-of-service attack but we can detect and respond to such attacks. The paper presents results of experiments showing that overall performance is good and that even a small window of vulnerability has little impact on service latency.
Circadian Clock Mutants in Arabidopsis Identified by Luciferase Imaging (1995)

The cycling bioluminescence of Arabidopsis plants carrying a firefly luciferase fusion construct was used to identify mutant individuals with aberrant cycling patterns. Both long- and short-period mutants were recovered. A semidominant short-period mutation, timing of CAB expression (toc1), was mapped to chromosome 5. The toc1 mutation shortens the period of two distinct circadian rhythms, the expression of chlorophyll a/b-binding protein (CAB) genes and the movements of primary leaves, although toc1 mutants do not show extensive pleiotropy for other phenotypes.
Feasibility of controlling prosthetic hand using sonomyography signal in real time: Preliminary study (2010)

The morphological changes of muscle can be accurately detected by sonography, a process we have termed sonomyography (SMG). This article investigates the feasibility of using muscle thickness deformation SMG as a new signal source to control a prosthetic hand in real time. Thickness deformation SMG of the extensor muscle was measured by a block-matching algorithm during wrist extension-flexion; the amplitude of the deformation was used to control the prosthetic hand. We compared various fast-search algorithms to select the best one for real-time prosthetic control. The two-dimensional logarithmic search (TDL) algorithm, with and without streaming single-instruction multiple-data extensions, showed excellent execution efficiency, with an overall mean correlation coefficient of about 0.99, a mean standard root-mean-square error <0.75, and a mean relative root-mean-square error <8.0% referenced to the cross-correlation algorithm baseline. The mean frame rates were greater than the ultrasound sampling rate (12 Hz), indicating that TDL could be implemented in real-time control. These results demonstrate that only one muscle position is needed to control a prosthetic hand, allowing for proprioception of muscle tension, and that the SMG provides good control of the prosthetic hand, allowing it to proportionally open and close with a fast-search algorithm.
A Practical Synthesis of (S)-2-Cyclohexyl-2-phenylglycolic Acid via Organocatalytic Asymmetric Construction of a Tetrasubstituted Carbon Center (2005)

[reaction: see text] A concise and enantioselective synthesis of (S)-2-cyclohexyl-2-phenylglycolic acid as a key intermediate for (S)-oxybutynin is reported. The crucial asymmetric tetrasubstituted carbon center was constructed with excellent stereoselectivity through the proline-catalyzed direct asymmetric aldol reaction between cyclohexanone and ethyl phenylglyoxylate under mild conditions.
Rapid patterning of ‘tunable’ hydrophobic valves on disposable microchips by laser printer lithography (2013)

We recently defined a method for fabricating multilayer microdevices using poly(ethylene terephthalate) transparency film and printer toner, and showed these could be successfully applied to DNA extraction and amplification (Duarte et al., Anal. Chem. 2011, 83, 5182-5189). Here, we advance the functionality of these microdevices with flow control enabled by hydrophobic valves patterned using laser printer lithography. Laser printer patterning of toner within the microchannel induces a dramatic change in surface hydrophobicity (change in contact angle of DI water from 51° to 111°) with good reproducibility. Moreover, the hydrophobicity of the surface can be controlled by altering the density of the patterned toner via varying the gray-scale setting on the laser printer, which consequently tunes the valve's burst pressure. Toner density provided a larger burst pressure bandwidth (158 ± 18 Pa to 573 ± 16 Pa) than could be achieved by varying channel geometry (492 ± 18 Pa to 573 ± 16 Pa). Finally, we used a series of tuned toner valves (with varied gray-scale) for passive valve-based fluidic transfer in a predictable manner through the architecture of a rotating PeT microdevice. While an elementary demonstration, this presents the possibility for simplistic and cost-effective microdevices with valved fluid flow control to be fabricated using nothing more than a laser printer, a laser cutter and a laminator.
Direct Measurement of Colloidal Forces in an Anisotropic Solvent (1997)

We present a new method to measure attractive interactions between colloidal particles, and determine the nature of the attraction between particles suspended in a nematic liquid crystal. We confine droplets filled with ferrofluid to a thin layer and apply a magnetic field to induce dipole moments that drive the droplets apart. When the field is removed, the attractive interactions pull the droplets back together. The force is determined from the velocity because the motion is viscously damped. We confirm the dipolar character of the interaction between droplets in a nematic solvent.
Congestion Control for Fair Resource Allocation in Networks With Multicast Flows (2004)

We consider the problem of congestion control in networks which support both multirate multicast sessions and unicast sessions. We present a decentralized algorithm which enables the different rate-adaptive receivers in different multicast sessions to adjust their rates to satisfy some fairness criterion. A one-bit ECN marking strategy to be used at the nodes is also proposed. The congestion-control mechanism does not require any per-flow state information for unicast flows at the nodes. At junctions nodes of each multicast tree, some state information about the rates along the branches at the node may be required. The congestion-control mechanism takes into account the diverse user requirements when different receivers within a multicast session have different utility functions, but does not require the network to have any knowledge about the receiver utility functions.
A Soluble Form of B Cell Maturation Antigen, a Receptor for the Tumor Necrosis Factor Family Member April, Inhibits Tumor Cell Growth (2000)

A proliferation-inducing ligand (APRIL) is a ligand of the tumor necrosis factor (TNF) family that stimulates tumor cell growth in vitro and in vivo. Expression of APRIL is highly upregulated in many tumors including colon and prostate carcinomas. Here we identify B cell maturation antigen (BCMA) and transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor (TACI), two predicted members of the TNF receptor family, as receptors for APRIL. APRIL binds BCMA with higher affinity than TACI. A soluble form of BCMA, which inhibits the proliferative activity of APRIL in vitro, decreases tumor cell proliferation in nude mice. Growth of HT29 colon carcinoma cells is blocked when mice are treated once per week with the soluble receptor. These results suggest an important role for APRIL in tumorigenesis and point towards a novel anticancer strategy.
Inhibition of Sphingosine 1-Phosphate Receptor 2 Protects against Renal Ischemia-Reperfusion Injury (2011)

Activation of the sphingosine 1-phosphate receptor 1 (S1P(1)R) protects against renal ischemia-reperfusion (IR) injury and inflammation, but the role of other members of this receptor family in modulating renal IR injury is unknown. We found that a selective S1P(2)R antagonist protected against renal IR injury in a dose-dependent manner. Consistent with this observation, both S1P(2)R-deficient mice and wild-type mice treated with S1P(2)R small interfering RNA had reduced renal injury after IR. In contrast, a selective S1P(2)R agonist exacerbated renal IR injury. The S1P(2)R antagonist increased sphingosine kinase-1 (SK1) expression via Rho kinase signaling in renal proximal tubules; the S1P(2)R agonist decreased SK1. S1P(2)R antagonism failed to protect the kidneys of SK1-deficient mice or wild-type mice pretreated with an SK1 inhibitor or an S1P(1)R antagonist, suggesting that the renoprotection conferred by S1P(2)R antagonism results from pathways involving activation of S1P(1)R by SK1. In cultured human proximal tubule (HK-2) cells, the S1P(2)R antagonist selectively upregulated SK1 and attenuated both H(2)O(2)-induced necrosis and TNF-α/cycloheximide-induced apoptosis; the S1P(2)R agonist had the opposite effects. In addition, increased nuclear hypoxia inducible factor-1α was critical in mediating the renoprotective effects of S1P(2)R inhibition. Finally, induction of SK1 and S1P(2)R in response to renal IR and S1P(2)R antagonism occurred selectively in renal proximal tubule cells but not in renal endothelial cells. Taken together, these data suggest that S1P(2)R may be a therapeutic target to attenuate the effects of renal IR injury.
Reflectometer measurements of density fluctuations in tokamak plasmas (invited) (1995)

Many anomalous features observed in reflectometer measurements of turbulent fluctuations in tokamak plasmas, such as loss of coherent reflection, large amplitude fluctuations, large angular divergence of the reflected waves, and correlation lengths of the order of the free-space wavelength of the probe beam, can be explained by modeling the plasma fluctuations as a poloidally varying random phase grating located at the cutoff with a phase magnitude given by 1D geometric optics. A key result of this analysis is that the turbulence spectrum cannot be inferred from phase measurements when large amplitude fluctuations are observed at the receiver. However, the turbulence spectrum may still be recovered from phase measurements by use of imaging optics and wide angle phase sensitive receivers.
Identity elements and aminoacylation of plant tRNATrp (1998)

Mutation of the Arabidopsis thaliana tRNA (Trp)(CCA) anticodon or of the A73 discriminator base greatly diminishes in vitro aminoacylation with tryptophan, indicating the importance of these nucleotides for recognition by the plant tryptophanyl-tRNA synthetase. Mutation of the tRNA (Trp)(CCA) anticodon to CUA so as to translate amber nonsense codons permits tRNA (Trp)(CCA) to be aminoacylated by A.thaliana lysyl-tRNA synthetase. Thus, translational suppression by tRNA (TRP)(CCA) observed in plant cells includes significant incorporation of lysine into protein.
Simple Procedure for Production by Group C Streptococci of Phage-Associated Lysin Active Against Group A Streptococci (1975)

Phage-associated lysin of high potency was prepared by growing the host group C streptococcal strain 26RP66 in a semisynthetic medium. The lysin was stabilized by adding dithiothreitol and neutralized ethylenediaminetetraacetic acid (EDTA) to facilitate further concentration and partial purification. The lysin remained active when stored at -65 C for 1 year. Lysin was active against all strains of group A streptococci tested and was more active against living cells than heat-killed cells. The procedure outlined is practicable for most bacteriological research laboratories and does not require column purification or other complex biochemical procedures. It should be useful to any laboratory which requires small amounts of lysin to produce L-forms and protoplasts or to release streptococcal antigens.
Tamper detection using neuro-fuzzy logic (1999)

This paper presents the application of a neuro-fuzzy model to tamper detection in static energy meters. TDNF software has been developed in C for the online detection of tamper events in static energy meters. This software provides an option to learn from the input pattern of electrical parameters during various field conditions. A fuzzy membership function is used to account for flexible boundary conditions of electrical parameters. Based upon the training of the neural network, it provides a log of temper events in a real-time domain. The results obtained through simulation are compared with practical conditions. It is observed that information provided by the proposed model is more meaningful as compared to that obtained through existing tamper detection algorithms.
Differential responses of human sperm to varying concentrations of pentoxyfylline with demonstration of toxicity. (1995)

Pentoxyfylline (PF), a methylxanthine derivative, is an inhibitor of the cAMP-phosphodiesterase enzyme, and is known to stimulate the motility of fresh and post-thaw human sperm. The purpose of this study was to examine the effects of different concentrations of PF on motility (MOT), path (curvilinear) velocity (PV), and hyperactivation (HA) of fresh sperm from patients (n = 24) and donors (n = 6) and post-thaw donor sperm (n = 5). For cryopreservation, the donor semen was frozen in liquid nitrogen using test-yolk-glycerol cryopreservative, stored for a minimum of 48 hours, then thawed at room temperature prior to assay. Aliquots of all samples to equal 10 x 10(6)/ml were diluted in 1 ml of the following: medium (human tubal fluid) only (control), or 2.5, 5, 10, or 20 mg/ml PF in medium. Specimens were incubated at 37 degrees C, and all were assessed by computer-assisted motion analysis at 0, 0.5, 1, and 2 hours. The patient specimens were divided into two groups: group 1, mean percent (standard deviation [SD]) MOT < 20% (12.8 +/- 5.8); group 2, mean percent (SD) MOT > 20% (37.8 +/- 14). For fresh donor sperm, 2.5 mg/ml PF significantly stimulated PV and HA at 0, 1, and 2 hours, and MOT at 0, 0.5, and 2 hours. PF at 5 mg/ml resulted in a decreased PV and HA, whereas MOT was decreased by 10 mg/ml. In the < 20% MOT group, 2.5 mg/ml PF significantly stimulated MOT at 0.5, 1, and 2 hours, and HA at 0 and 2 hours. There was no effect on PV.(ABSTRACT TRUNCATED AT 250 WORDS)
Integrated Modeling of the El Furrial Field Asset Applying Risk and Uncertainty Analysis for the Decision Making (2005)

Abstract The El Furrial field is one most important hydrocarbon giants of Venezuela. It's located at the north of the Monagas State and it handles approximated 45 % of the production of this area. It was discovered in 1986 and its characteristic makes it unique. The strategy of development has been supported by the combined injection of miscible gas and water; this has allowed maintaining the production plateau of 400 MBPD of 28 ° API oil. A transcendental advance in the characterization of the field was done with a new dynamic model called V9, what promoted the evaluation of new development scenario. This paper presents one of the first integrated development plan built by PDVSA; focus on the Integrated Modeling of the Asset, considering risk and uncertainty analysis in the decision taking. The project was developed under a philosophy of collaborative work and changing the way of thinking from deterministic to probabilistic. The Project was executed by a multidisciplinary team integrated by personnel of the core areas of the asset, creating a milestone in the traditional way of working. This integration allowed evaluation through statistic and expert interview, the uncertainties associated to the variables involved in the evaluated scenarios. Huge reduction of capital expenses related with the grow of facilities were obtain due to the estimation of production profiles based on risk and uncertainty analysis. As a result of this analysis a decision matrix was built, with the recovery factor, investment, qualitative risk factor, and economic indexes, which were evaluated to decide the optimum development plan.
Repair of Infarcted Myocardium by Autologous Intracoronary Mononuclear Bone Marrow Cell Transplantation in Humans (2002)

These results demonstrate for the first time that selective intracoronary transplantation of autologous, mononuclear BMCs is safe and seems to be effective under clinical conditions. The marked therapeutic effect may be attributed to BMC-associated myocardial regeneration and neovascularization.