gebhart/patrepeval huggingface dataset:
Device for thermally-induced vapor phase transfer of adsorbed organics directly from an adsorbent to a gas chromatograph-mass spectrometer (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDevice for thermally-induced vapor phase transfer of adsorbed organics directly from an adsorbent to a gas chromatograph-mass spectrometerWoodfin V. Ligon and Robert L. JohnsonCite this: Anal. Chem. 1976, 48, 3, 481–484Publication Date (Print):March 1, 1976Publication History Published online1 May 2002Published inissue 1 March 1976https://pubs.acs.org/doi/10.1021/ac60367a035https://doi.org/10.1021/ac60367a035research-articleACS PublicationsRequest reuse permissionsArticle Views28Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Isolation and characterization of a colonic autoantigen specifically recognized by colon tissue-bound immunoglobulin G from idiopathic ulcerative colitis. (1985)

Patients with idiopathic ulcerative colitis (UC) have a colon- bound antibody (CCA-IgG) that reacts with colon tissue extracts. We have partially characterized a colonic protein that is specifically recognized by CCA-IgG. CCA-IgG was eluted from operative colon specimens from 10 patients with UC. A colon tissue-bound IgG was similarly eluted from six patients with Crohn's colitis, two with ischemic colitis, and one with diver- ticulitis. Purified serum IgG from patients with Crohn's disease, from normal subjects and a patient with myeloma were also used as additional controls. For detection of antigen(s), tissue extracts were prepared from 26 specimens of colon (UC, 12; Crohn's disease, 6; normal, 4; other controls, 4), 8 specimens of human normal stomach, duodenum, ileum, and liver (2 each). Tissue extracts were also prepared from rats and mice, including germ-free rat colons and rat's fetal colons. Immunorecognition of CCA-IgG to the tissue extracts was examined by affinity-column chromatography and by transblot analysis. Tissue-extracted proteins were electrophoresed in SDS-polyacrylamide gel, transferred to nitrocellulose sheet, and probed with iodinated CCA-IgG, colonic IgG from other inflammatory bowel disease patients, UC serum IgG, and control serum IgG. Although many proteins were present in colon tissue extracts, 9 of 10 CCA-IgG consistently recognized a protein of 40 kD. None of the nine IgG preparations from colon specimens of patients with Crohn's colitis and other colonic inflammatory diseases reacted with the 40-kD protein. Five of six symptomatic UC serum IgG and none of eight control serum IgG reacted with the 40-kD protein. The 40-kD protein was present in all colon specimens and it appeared to be organ specific. It was absent in mouse and rat tissues, including colon. The 40-kD protein is not actin and nor a part of the Ig molecule. These results suggest that the 40-kD protein is a colonic "autoantigen" that may initiate a specific IgG antibody response in UC.
Enhancing and suppressing effects of recombinant murine macrophage inflammatory proteins on colony formation in vitro by bone marrow myeloid progenitor cells (1990)

Purified recombinant (r) macrophage inflammatory proteins (MIPs) 1 alpha, 1 beta, and 2 were assessed for effects on murine (mu) and human (hu) marrow colony-forming unit-granulocyte-macrophage (CFU-GM) and burst-forming unit-erythroid (BFU-E) colonies. Recombinant MIP-1 alpha, -1 beta, and -2 enhanced muCFU-GM colonies above that stimulated with 10 to 100 U natural mu macrophage-colony-stimulating factor (M-CSF) or rmuGM-CSF, with enhancement seen on huCFU-GM colony formation stimulated with suboptimal rhuM-CSF or rhuGM-CSF; effects were neutralized by respective MIP-specific antibodies. Macrophage inflammatory proteins had no effects on mu or huBFU-E colonies stimulated with erythropoietin (Epo). However, natural MIP-1 and rMIP-1 alpha, but not rMIP-1 beta or -2, suppressed muCFU-GM stimulated with pokeweed mitogen spleen-conditioned medium (PWMSCM), huCFU-GM stimulated with optimal rhuGM-CSF plus rhu interleukin-3 (IL-3), muBFU-E and multipotential progenitors (CFU-GEMM) stimulated with Epo plus PWMSCM, and huBFU-E and CFU-GEMM stimulated with Epo plus rhuIL-3 or rhuGM-CSF. The suppressive effects of natural MIP-1 and rMIP-1 alpha were also apparent on a population of BFU-E, CFU-GEMM, and CFU-GM present in cell-sorted fractions of human bone marrow (CD34 HLA-DR+) highly enriched for progenitors with cloning efficiencies of 42% to 75%. These results, along with our previous studies, suggest that MIP-1 alpha, -1 beta, and -2 may have direct myelopoietic enhancing activity for mature progenitors, while MIP-1 alpha may have direct suppressing activity for more immature progenitors.
Effect of Active Workstation on Energy Expenditure and Job Performance: A Systematic Review and Meta-analysis (2015)

Active workstation could significantly increase daily PA and be potentially useful in reducing workplace sedentariness. Although some parts of job performance were significantly lower, others were not. As a result there was little effect on real-life work productivity if we made a good arrangement of job tasks.
International Evidenceon the Determinants of Private Saving (1995)

A broad set of possible determinants of private saving behavior is examined, using data for a large sample of industrial and developing countries. Both time-series and cross-section estimates are obtained. Results suggest that there is a partial offset on private saving of changes in public saving and (for developing countries) in foreign saving, that demographics and growth are important determinants of private saving rates, and that interest rates and terms of trade have positive, but less robust, effects. Increases in per capita GDP seem to increase saving at low income levels (relative to the United States) but decrease it at higher ones.
Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease (2020)

This study demonstrates that inhibition of myostatin does not revert muscle atrophy in a mouse model with established OPMD disease, but is effective at reducing observed histological markers of fibrosis in the treated muscles.
Application of laser photofragmentation-resonance enhanced multiphoton ionization to ion mobility spectrometry (2010)

We demonstrate detection of nitro-containing compounds with laser photofragmentation (PF) coupled with resonance enhanced multiphoton ionization (REMPI) and ion mobility spectrometry (IMS). In PF-REMPI, a laser dissociates the parent molecules, producing fragments that can then be ionized by absorption of additional laser photons. The production of these ions strongly depends on the wavelength of laser light, with ion yields corresponding to the absorption spectrum of the fragments [nitric oxide (NO) in the present case]. Combining IMS with PF-REMPI provides further specificity, separating ions according to their mobilities through an atmospheric-pressure drift tube. In this work, we use a pulsed UV laser to examine the characteristics of atmospheric-pressure PF-REMPI, the chemistry occurring in the ionization region and drift tube, and the viability of detecting ions created by both resonance-enhanced and nonresonant ionization. Probing NO in a helium-nitrogen bath, we demonstrate that the detection of ions displays single-shot response to changes in ion generation, with an ion extraction-to-collection efficiency of approximately 12%. We then evaluate the sensitivity and specificity of PF-REMPI/IMS as applied to the detection of both the explosive surrogate 2, 4-dinitrotoluene and the nuisance compound nitrobenzene.
A comprehensive guide to antibody and T‐cell responses in type 1 diabetes (2003)

Type 1 diabetes (T1D) is an organ-specific autoimmune disease in which the insulin-producing beta cells in the pancreatic islets are selectively eliminated. T cells specific for beta-cell antigens are the mediators of this precise cellular destruction. However, antibodies to beta-cell proteins are also generated and may be used for predicting disease in at-risk populations. Over the past two decades, numerous beta-cell proteins and lipids have been implicated as autoantigens in patients or in non-obese diabetic (NOD) mice, a well-studied animal model of T1D. Here, we present a review of these antigens, accompanied by their T-cell epitopes, where known, and a discussion of our current understanding of why particular self-proteins become disease-inciting antigens. Although two dozen beta-cell antigens have been identified to date, few of these have been confirmed to be recognized by pathogenic T cells early in the disease process. Further identification and characterization of initiating beta-cell antigens targeted by pathogenic T cells should be a priority for future studies.
Gefitinib Inhibits the Proliferation of Pancreatic Cancer Cells via Cell Cycle Arrest (2009)

High expression of the epidermal growth factor receptor (EGFR) has been implicated in the development of pancreatic cancer. Gefitinib is an orally active and selective EGFR-TKI (EGFR-tyrosine kinase inhibitor) that blocks signal transduction pathways responsible for the proliferation and survival of cancer cells, and other host-dependent processes promoting cancer growth. This study investigated the anticancer effect of gefitinib on human pancreatic cancer cells and the molecular mechanism involved. We first evaluated the effect of gefitinib on cell proliferation with MTT assay and the results demonstrated that gefitinib significantly inhibited the proliferation of pancreatic cancer cells. Flow cytometric analysis showed that gefitinib induced a delay in cell cycle progression and a G0/G1 arrest together with a G2/M block; these were associated with increased expression of p27(Kip1) cyclin-dependent kinase inhibitor combined with decreased expression of aurora B. Besides, luciferase reporter assay revealed that transcriptional mechanism was responsible for the down-regulation of aurora B protein by gefitinib. Overall, the results suggest a mechanistic connection among these events to provide new insights into the mechanism underlying the antiproliferative effect of gefitinib on pancreatic cancer and supplement a theory basis of gefitinib in clinical treatment of pancreatic cancer.
Glycerol/PEDOT:PSS coated woven fabric as a flexible heating element on textiles (2017)

A cost-competitive, flexible and safe thermoelectric polyamide 6,6 (PA66) fabric coated with glycerol-doped PEDOT:PSS (PEDOT:PSS + GLY) for use in large area textiles as a heating element in several applications.
Thalidomide Enhances the Anti-Tumor Activity of Standard Chemotherapy in a Human Melanoma Xenotransplatation Model (2005)

It has been demonstrated that thalidomide's anti-angiogenic properties result in clear anti-tumor activity in a number of human malignancies. We studied thalidomide in a human melanoma severe combined immunodeficiency mouse xenotransplantation model. Thalidomide as a single agent showed a significant tumor reduction of 46% compared with the control group. Thalidomide combined with dacarbazine treatment markedly enhanced the anti-tumor effect of chemotherapy and showed a significant tumor reduction relative to the dacarbazine-only group (61%) and even more tumor reduction (74%) compared with the control group. We also measured clearly reduced levels of tumor necrosis factor-alpha in the thalidomide-treated group. A significantly lower microvessel density was encountered in the thalidomide treatment groups (thalidomide alone or combined with DTIC), underscoring the anti-angiogenic effect of thalidomide as a single agent as well as in combination with chemotherapy in this model. In line with these results, we observed a nearly 3-fold increase of apoptosis for the combination of thalidomide and DTIC compared with the rate of apoptotic cells in DTIC-only-treated melanoma xenotransplants. These data underline the rationale for combining dacarbazine--a cytotoxic agent--and thalidomide--an anti-angiogenic cytostatic agent--as a promising strategy for the treatment of melanoma.
High-Resolution MRI of Carotid Plaque With a Neurovascular Coil and Contrast-Enhanced MR Angiography: One-Stop Shopping for the Comprehensive Assessment of Carotid Atherosclerosis (2011)

In patients with carotid atherosclerosis, ongoing CE-MRA with a neurovascular coil for the simultaneous detection of unstable plaques is feasible. Our MR protocol accurately identifies the major features of vulnerable plaque.
Kaposi's sarcoma-associated herpesvirus in non-AIDS related lymphomas occurring in body cavities. (1996)

DNA sequences belonging to the recently discovered Kaposi's sarcoma-associated herpesvirus (KSHV), now provisionally designated human herpesvirus 8, have been previously identified in an uncommonly occurring subset of AIDS-related lymphomas, referred to as body-cavity-based lymphomas (BCBLs), which present as lymphomatous effusions. Pyothorax-associated lymphomas (PALS) are non-Hodgkin's lymphomas that arise in the pleural cavity after long-standing pleural inflammation resulting from therapeutic artificial pneumothorax or from tuberculosis pleuritis. Although PALs present as solid tumor masses, they are otherwise similar to BCBLs in that they also are B cell lymphomas, usually exhibit immunoblastic morphology, and contain Epstein-Barr virus. We investigated whether KSHV sequences are present in 2 BCBLs in patients without AIDS and 12 in Japanese and 2 French PALs. The 2 BCBLs were positive for KSHV sequences, whereaas all 14 PALs were KSHV negative. This finding strongly suggests that BCBLs and PALs are distinct clinicopathological entities and further strengthens the association between the presence of KSHV and an effusion phenotype. Based on these findings, we propose replacing the term body-cavity-based lymphoma with the term primary effusion lymphoma, which describes these non-Hodgkin's lymphomas more accurately and avoids confusion with other lymphomas that may occur in the body cavities, such as the PALs.
Crystallization and preliminary crystallographic characterization of recombinantL-methionine-α-deamino-γ-mercaptomethane lyase (methioninase) (2000)

L-Methionine-alpha-deamino-gamma-mercaptomethane lyase (rMETase) is involved in the alpha,gamma-elimination of methionine to alpha-ketobutyrate, methanethiol and ammonia. The reaction catalyzed by rMETase reduces the methionine concentration of methionine-dependent tumor cells, arresting their growth. Towards the goal of developing rMETase into an effective antitumor therapeutic and also to understand the catalytic mechanism of this enzyme, rMETase from Pseudomonas putida has been expressed, purified and crystallized. The crystals belong to space group P2(1)2(1)2 and diffract X-rays to at least 2.68 A resolution at 100 K using synchrotron radiation. The unit cell has parameters a = 152.8, b = 154.6, c = 80.8 A and contains four molecules in the asymmetric unit.
2000 Update of American Society of Clinical Oncology Colorectal Cancer Surveillance Guidelines (2000)

Article Tools ASCO SPECIAL ARTICLE Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2000.18.20.3586 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 11032600 2000 Update of American Society of Clinical Oncology Colorectal Cancer Surveillance Guidelines Al B. Benson IIIxAl B. Benson IIISearch for articles by this author , Christopher E. DeschxChristopher E. DeschSearch for articles by this author , Patrick J. FlynnxPatrick J. FlynnSearch for articles by this author , Carol KrausexCarol KrauseSearch for articles by this author , Charles L. LoprinzixCharles L. LoprinziSearch for articles by this author , Bruce D. MinskyxBruce D. MinskySearch for articles by this author , Nicholas J. PetrellixNicholas J. PetrelliSearch for articles by this author , David G. PfisterxDavid G. PfisterSearch for articles by this author , Thomas J. SmithxThomas J. SmithSearch for articles by this author , Mark R. SomerfieldxMark R. SomerfieldSearch for articles by this author , for the American Society of Clinical OncologyxSearch for articles by this author Show More From the American Society of Clinical Oncology, Alexandria, VA. https://doi.org/10.1200/JCO.2000.18.20.3586 First Page Full Text PDF Figures and Tables © 2000 by American Society of Clinical Oncology
Pilot Contamination Elimination for Large-Scale Multiple-Antenna Aided OFDM Systems (2014)

Pilot contamination constitutes a particularly significant impairment in large-scale multi-cell systems. We propose an effective pilot contamination elimination scheme for multi-cell time division duplexing based orthogonal frequency division multiplexing systems, by carefully designing a sophisticated amalgam of downlink (DL) training and ‘scheduled’ uplink (UL) training. During the DL training stage, each base station (BS) transmits the DL pilot symbols (PSs) to its mobile stations (MSs) for them to estimate their frequency-domain channel transfer functions (FDCHTFs), which are then embedded in the UL PSs by ‘predistorting’ the PSs with the estimated FDCHTFs. During the scheduled UL training, each BS's UL receiver in turn extracts the FDCHTFs of its MSs from their received PSs by eliminating the pilot contamination imposed by the simultaneously transmitted UL PSs of all other cells. Our simulation results demonstrate that the pilot contamination is completely eliminated by the proposed scheme, even for the network consisting of a large number of unity frequency reuse cells. Most significantly, unlike many existing pilot contamination reduction schemes, our scheme does not rely on the assumption that each BS knows the second-order statistics of all the interfering UL channels.
CD4+ cutaneous T-cell lymphoma cells express the p140–killer cell immunoglobulin-like receptor (2001)

Tumor cells of patients with cutaneous T-cell lymphoma (CTCL) have the cell surface phenotype of mature T-helper lymphocytes, and it may be impossible to differentiate them from nonmalignant lymphocytes in skin and blood. Until now, no specific cell membrane marker of CTCL has been reported. In the current study, it is reported for the first time that CTCL cells express the major histocompatibility complex class I binding p140-killer cell immunoglobulin-like receptor, which has been described on a minor subset of natural killer lymphocytes and on a marginal circulating CD8(+) T lymphocyte subset. Interestingly, the molecular characterization of this KIR expressed by CTCL allowed us to isolate a novel allelic form of p140-KIR3DL, resulting in 4 amino acid substitutions, 3 in the extracellular immunoglobulin-like domain of the protein and one in the cytoplasmic region. This finding is likely to be important both for the pathophysiology and for the clinical treatment of patients with CTCL.
Soluble domains of telomerase reverse transcriptase identified by high‐throughput screening (2005)

Telomerase is a ribonucleoprotein complex responsible for extending the ends of eukaryotic chromosomes. Structural and biophysical studies of this enzyme have been limited by the inability to produce large amounts of recombinant protein. Here we perform a high-throughput screen to map regions of the Tetrahymena thermophila TERT (Telomerase Reverse Transcriptase) protein that are overexpressed in a soluble form in Escherichia coli using a GFP-fusion system. Many of the soluble protein domains identified do not coincide with domains inferred from multiple sequence alignment, so screening for fluorescent colonies provided information not otherwise readily obtained. The method revealed an essential, independently folded N-terminal domain that was expressed and purified with high yield and found to be suitable for structural analysis. These results provide a tool for future structural and biophysical studies of TERT.
B7-H1 blockade augments adoptive T-cell immunotherapy for squamous cell carcinoma. (2003)

In this report, we demonstrate that B7-H1, a B7 family molecule implicated in tumor immune evasion, is constitutively expressed on 66% of freshly isolated squamous cell carcinomas of the head and neck (SCCHN). To define the potential impact of tumor-associated B7-H1 on immunotherapy, the B7-H1-negative mouse SCC line, SCCVII, was transfected to express B7-H1. Although all of the animals succumbed to B7-H1/SCCVII tumors even after adoptive T-cell immunotherapy, the infusion of B7-H1 blocking monoclonal antibody with activated T cells cured 60% of animals. These data support B7-H1 blockade as a new approach to enhance the efficacy of T-cell immunotherapy.
Flexible switching and editing of MPEG-2 video bitstreams (1997)

This paper proposes and compares solutions for switching and editing of compressed video signals in the programme chain. Proposals for bitstream splicing have been made, but these lack flexibility. Also, switching in the decoded domain degrades picture quality. The ACTS ATLANTIC project has developed an alternative approach, in which the decoded signals are switched, but recording makes intelligent use of the coding decisions used to create the original bitstreams. This paper outlines the techniques used in this approach, and gives results of simulations. Examples of implementations for opt-out and editing applications are given. The paper proposes techniques which allows the frame-accurate switching and editing of MPEG-2 bitstreams without introducing any visible impairment. The techniques are also applicable to other types of compression.
An analysis of protein domain linkers: their classification and role in protein folding (2002)

Recent advances in protein engineering have come from creating multi-functional chimeric proteins containing modules from various proteins. These modules are typically joined via an oligopeptide linker, the correct design of which is crucial for the desired function of the chimeric protein. Here we analyse the properties of naturally occurring inter-domain linkers with the aim to design linkers for domain fusion. Two main types of linker were identified; helical and non-helical. Helical linkers are thought to act as rigid spacers separating two domains. Non-helical linkers are rich in prolines, which also leads to structural rigidity and isolation of the linker from the attached domains. This means that both linker types are likely to act as a scaffold to prevent unfavourable interactions between folding domains. Based on these results we have constructed a linker database intended for the rational design of linkers for domain fusion, which can be accessed via the Internet at http://mathbio.nimr.mrc.ac.uk.
Harmonic generation of an Yb-doped photonic-crystal fiber amplifier to obtain 1ns pulses of 410, 160, and 190kW peak-power at 531, 354, and 265nm wavelength (2006)

By frequency doubling, tripling, and quadrupling 1ns, ~10kHz-repetition-rate pulses from a 1062nm-wavelength Yb-doped photonic crystal fiber amplifier, we obtained pulse peak/average powers of 410kW/4W at 531nm, 160kW/1.5W at 354nm, and 190kW/1.8W at 265.5nm.