gebhart/patrepeval huggingface dataset:
Mutational Study on the Roles of Disulfide Bonds in the β-Subunit of Gastric H+,K+-ATPase (2002)

The gastric proton pump, H(+),K(+)-ATPase, consists of the catalytic alpha-subunit and the non-catalytic beta-subunit. Correct assembly between the alpha- and beta-subunits is essential for the functional expression of H(+),K(+)-ATPase. The beta-subunit contains nine conserved cysteine residues; two are in the cytoplasmic domain, one in the transmembrane domain, and six in the ectodomain. The six cysteine residues in the ectodomain form three disulfide bonds. In this study, we replaced each of the cysteine residues of the beta-subunit with serine individually and in several combinations. The mutant beta-subunits were co-expressed with the alpha-subunit in human embryonic kidney 293 cells, and the role of each cysteine residue or disulfide bond in the alpha/beta assembly, stability, and cell surface delivery of the alpha- and beta-subunits and H(+),K(+)-ATPase activity was studied. Mutant beta-subunits with a replacement of the cytoplasmic and transmembrane cysteines preserved H(+),K(+)-ATPase activity. All the mutant beta-subunits with replacement(s) of the extracellular cysteines did not assemble with the alpha-subunit, resulting in loss of H(+),K(+)-ATPase activity. These mutants did not permit delivery of the alpha-subunit to the cell surface. Therefore, each of these disulfide bonds of the beta-subunit is essential for assembly with the alpha-subunit and expression of H(+),K(+)-ATPase activity as well as for cell surface delivery of the alpha-subunit.
Mastering Data Mining: The Art and Science of Customer Relationship Management (2000)

From the Publisher:
Berry and Linoff lead the reader down an enlightened path of best practices. -Dr. Jim Goodnight, President and Cofounder, SAS Institute Inc.This is a great book, and it will be in my stack of four or five essential resources for my professional work. -Ralph Kimball, Author of The Data Warehouse Lifecycle ToolkitMastering Data MiningIn this follow-up to their successful first book, Data Mining Techniques, Michael J. A. Berry and Gordon S. Linoff offer a case study-based guide to best practices in commercial data mining. Their first book acquainted you with the new generation of data mining tools and techniques and showed you how to use them to make better business decisions. Mastering Data Mining shifts the focus from understanding data mining techniques to achieving business results, placing particular emphasis on customer relationship management.In this book, you'll learn how to apply data mining techniques to solve practical business problems. After providing the fundamental principles of data mining and customer relationship management, Berry and Linoff share the lessons they have learned through a series of warts-and-all case studies drawn from their experience in a variety of industries, including e-commerce, banking, cataloging, retailing, and telecommunications.Through the cases, you will learn how to formulate the business problem, analyze the data, evaluate the results, and utilize this information for similar business problems in different industries.Berry and Linoff show you how to use data mining to:* Retain customer loyalty* Target the right prospects* Identify new markets for products and services* Recognize cross-selling opportunities on and off the Web. Thecompanion Web site features:* Updated information on data mining products and service providers* Information on data mining conferences, courses, and other sources of information* Full-color versions of the illustrations used in the book
5S Ribosomal RNA Database (2002)

Ribosomal 5S RNA (5S rRNA) is an integral component of the large ribosomal subunit in all known organisms with the exception only of mitochondrial ribosomes of fungi and animals. It is thought to enhance protein synthesis by stabilization of a ribosome structure. This paper presents the updated database of 5S rRNA and their genes (5S rDNA). Its short characteristics are presented in the Introduction. The database contains 2280 primary structures of 5S rRNA and 5S rRNA genes. These include 536 eubacterial, 61 archaebacterial, 1611 eukaryotic and 72 organelle sequences. The database is available on line through the World Wide Web at http://biobases.ibch.poznan.pl/5SData/.
Untersuchungen zur molekularen Dynamik von Tetraethyloxysilan unterhalb des Gefrierpunktes (1982)

Article Untersuchungen zur molekularen Dynamik von Tetraethyloxysilan unterhalb des Gefrierpunktes was published on January 1, 1982 in the journal Zeitschrift für Physikalische Chemie (volume 263O, issue 1).
Long-lived epithelial immunity by tissue-resident memory T (T RM ) cells in the absence of persisting local antigen presentation (2012)

Although circulating memory T cells provide enhanced protection against pathogen challenge, they often fail to do so if infection is localized to peripheral or extralymphoid compartments. In those cases, it is T cells already resident at the site of virus challenge that offer superior immune protection. These tissue-resident memory T (T(RM)) cells are identified by their expression of the α-chain from the integrin α(E)(CD103)β(7), and can exist in disequilibrium with the blood, remaining in the local environment long after peripheral infections subside. In this study, we demonstrate that long-lived intraepithelial CD103(+)CD8(+) T(RM) cells can be generated in the absence of in situ antigen recognition. Local inflammation in skin and mucosa alone resulted in enhanced recruitment of effector populations and their conversion to the T(RM) phenotype. The CD8(+) T(RM) cells lodged in these barrier tissues provided long-lived protection against local challenge with herpes simplex virus in skin and vagina challenge models, and were clearly superior to the circulating memory T-cell cohort. The results demonstrate that peripheral T(RM) cells can be generated and survive in the absence of local antigen presentation and provide a powerful means of achieving immune protection against peripheral infection.
Interactive sound propagation using compact acoustic transfer operators (2012)

We present an interactive sound propagation algorithm that can compute high orders of specular and diffuse reflections as well as edge diffractions in response to moving sound sources and a moving listener. Our formulation is based on a precomputed acoustic transfer operator, which we compactly represent using the Karhunen-Loeve transform. At runtime, we use a two-pass approach that combines acoustic radiance transfer with interactive ray tracing to compute early reflections as well as higher-order reflections and late reverberation. The overall approach allows accuracy to be traded off for improved performance at runtime, and has a low memory overhead. We demonstrate the performance of our algorithm on different scenarios, including an integration of our algorithm with Valve's Source game engine.
Estimated distribution and movement of hairtail Trichiurus lepturus in the Aru Sea, based on the logbook records of trawlers (2001)

The distribution and movement of the hairtail Trichiurus lepturus stock in the Aru Sea were investigated using logbook data from four Taiwanese commercial otter trawlers. Fluctuations in population size, population density index and the distribution of catch per unit effort (CPUE) and fishing effort are discussed. The CPUE data were standardized by using the generalized linear model (GLM) in which fishing month, fishing area and size category of the hairtail were taken into account. The fishing season could be divided into three episodes: aggregation between March and April, dispersion between May and June, and aggregation again between July and December. A seasonal two-peak distribution of the population size of hairtail reflects the aggregation-dispersion pattern. The first peak in May-June can be explained in terms of the hairtails' overwintering migration. The second peak in October-December is likely to be the result of spawning aggregation.
Relationship Between Major Vault Protein/Lung Resistance Protein, Multidrug Resistance-Associated Protein, P-Glycoprotein Expression, and Drug Resistance in Childhood Leukemia (1998)

Cellular drug resistance is related to a poor prognosis in childhood leukemia, but little is known about the underlying mechanisms. We studied the expression of P-glycoprotein (P-gp), multidrug resistance (MDR)-associated protein (MRP), and major vault protein/lung resistance protein (LRP) in 141 children with acute lymphoblastic leukemia (ALL) and 27 with acute myeloid leukemia (AML) by flow cytometry. The expression was compared between different types of leukemia and was studied in relation with clinical risk indicators and in vitro cytotoxicity of the MDR-related drugs daunorubicin (DNR), vincristine (VCR), and etoposide (VP16) and the non-MDR-related drugs prednisolone (PRD) and L-asparaginase (ASP). In ALL, P-gp, MRP, and LRP expression did not differ between 112 initial and 29 unrelated relapse samples nor between paired initial and relapse samples from 9 patients. In multiple relapse samples, LRP expression was 1.6-fold higher compared with both initial (P = .026) and first relapse samples (P = .050), which was not observed for P-gp and MRP. LRP expression was weakly but significantly related to in vitro resistance to DNR (Spearman's rank correlation coefficient 0.25, P = .016) but not to VCR, VP16, PRD, and ASP. No significant correlations were found between P-gp or MRP expression and in vitro drug resistance. Samples with a marked expression of two or three resistance proteins did not show increased resistance to the tested drugs compared with the remaining samples. The expression of P-gp, MRP, and LRP was not higher in initial ALL patients with prognostically unfavorable immunophenotype, white blood cell count, or age. The expression of P-gp and MRP in 20 initial AML samples did not differ or was even lower compared with 112 initial ALL samples. However, LRP expression was twofold higher in the AML samples (P < .001), which are more resistant to a variety of drugs compared with ALL samples. In conclusion, P-gp and MRP are unlikely to be involved in drug resistance in childhood leukemia. LRP might contribute to drug resistance but only in specific subsets of children with leukemia.
[Protective effect of ONO-1078, a leukotriene receptor antagonist, on focal cerebral ischemia induced by endothelin-1 in rats]. (2004)

ONO-1078 possesses neuroprotective effect against cerebral ischemic injury induced by endothelin-1, therefore, leukotrienes may play a role in the injury of cerebral ischemia.
A Study of Neural Word Embeddings for Named Entity Recognition in Clinical Text. (2015)

Clinical Named Entity Recognition (NER) is a critical task for extracting important patient information from clinical text to support clinical and translational research. This study explored the neural word embeddings derived from a large unlabeled clinical corpus for clinical NER. We systematically compared two neural word embedding algorithms and three different strategies for deriving distributed word representations. Two neural word embeddings were derived from the unlabeled Multiparameter Intelligent Monitoring in Intensive Care (MIMIC) II corpus (403,871 notes). The results from both 2010 i2b2 and 2014 Semantic Evaluation (SemEval) data showed that the binarized word embedding features outperformed other strategies for deriving distributed word representations. The binarized embedding features improved the F1-score of the Conditional Random Fields based clinical NER system by 2.3% on i2b2 data and 2.4% on SemEval data. The combined feature from the binarized embeddings and the Brown clusters improved the F1-score of the clinical NER system by 2.9% on i2b2 data and 2.7% on SemEval data. Our study also showed that the distributed word embedding features derived from a large unlabeled corpus can be better than the widely used Brown clusters. Further analysis found that the neural word embeddings captured a wide range of semantic relations, which could be discretized into distributed word representations to benefit the clinical NER system. The low-cost distributed feature representation can be adapted to any other clinical natural language processing research.
Modeling compressive strength of recycled aggregate concrete by Artificial Neural Network, Model Tree and Non-linear Regression (2014)

In the recent past Artificial Neural Networks (ANN) have emerged out as a promising technique for predicting compressive strength of concrete. In the present study back propagation was used to predict the 28 day compressive strength of recycled aggregate concrete (RAC) along with two other data driven techniques namely Model Tree (MT) and Non-linear Regression (NLR). Recycled aggregate is the current need of the hour owing to its environmental friendly aspect of re-use of the construction waste. The study observed that, prediction of 28 day compressive strength of RAC was done better by ANN than NLR and MT. The input parameters were cubic meter proportions of Cement, Natural fine aggregate, Natural coarse Aggregates, recycled aggregates, Admixture and Water (also called as raw data). The study also concluded that ANN performs better when non-dimensional parameters like Sand–Aggregate ratio, Water–total materials ratio, Aggregate–Cement ratio, Water–Cement ratio and Replacement ratio of natural aggregates by recycled aggregates, were used as additional input parameters. Study of each network developed using raw data and each non dimensional parameter facilitated in studying the impact of each parameter on the performance of the models developed using ANN, MT and NLR as well as performance of the ANN models developed with limited number of inputs. The results indicate that ANN learn from the examples and grasp the fundamental domain rules governing strength of concrete.
Cutting Edge: Inflammatory Responses Can Be Triggered by TREM-1, a Novel Receptor Expressed on Neutrophils and Monocytes (2000)

We have identified new activating receptors of the Ig superfamily expressed on human myeloid cells, called TREM (triggering receptor expressed on myeloid cells). TREM-1 is selectively expressed on blood neutrophils and a subset of monocytes and is up-regulated by bacterial LPS. Engagement of TREM-1 triggers secretion of IL-8, monocyte chemotactic protein-1, and TNF-alpha and induces neutrophil degranulation. Intracellularly, TREM-1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-gamma. To mediate activation, TREM-1 associates with the transmembrane adapter molecule DAP12. Thus, TREM-1 mediates activation of neutrophil and monocytes, and may have a predominant role in inflammatory responses.
Identification of human rotavirus serotype by hybridization to polymerase chain reaction-generated probes derived from a hyperdivergent region of the gene encoding outer capsid protein VP7 (1990)

We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 could be used as templates to amplify this region. High-stringency hybridization of each of the four probes to rotavirus RNAs dotted on nylon membranes allowed the specific detection of corresponding sequences and thus permitted identification of the serotype of the strains dotted. The procedure was useful when applied to rotaviruses isolated from field studies.
Abnormal Grignard Reactions. XII.1 Sterically Hindered Aliphatic Carbonyl Compounds. II.1 Ketones Containing the Dineopentylcarbinyl Group (1942)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAbnormal Grignard Reactions. XII.1 Sterically Hindered Aliphatic Carbonyl Compounds. II.1 Ketones Containing the Dineopentylcarbinyl GroupFrank C. Whitmore and Charles T. LesterCite this: J. Am. Chem. Soc. 1942, 64, 6, 1247–1251Publication Date (Print):June 1, 1942Publication History Published online1 May 2002Published inissue 1 June 1942https://pubs.acs.org/doi/10.1021/ja01258a003https://doi.org/10.1021/ja01258a003research-articleACS PublicationsRequest reuse permissionsArticle Views256Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Important Dimensions of Wine Tourism Experience from Potential Visitors' Perception (2009)

The objective of this study is to identify the salient features of wineries and wine regions, from the perspective of a potential Israeli visitor. This insight facilitates an understanding of the features that might influence the decision to visit a winery or wine region, and enables operators to develop varied wine tourism products, combined with other attractions and activities. An exploratory study has been conducted and included 373 valid questionnaires of adult Israeli residents. The questionnaires included a list of 42 winery and wine region features, based on previous literature and preliminary semi-structured interviews. Participants were asked to rate the importance of each feature with respect to their decision to visit wineries or wine regions (on a 1-5 Likert-type scale). Factor analysis technique was implemented resulting in three factors, representing different dimensions of the wine tourism experience, and explained 43.9 per cent of the total variance. Factor scores were calculated and used for comparisons between different segments. The findings indicated that different segments yielded different importance ratings for each dimension. Fundamental differences occurred among specific segment bases: winery visitation frequency, subjects having children less than 18 years and wine involvement. Results show that the motivations for choosing a wine region as a tourist destination go beyond a simple desire to experience wine. A tourist's decision to visit a specific winery is also shaped by the willingness to experience the region's features and to participate in other activities or attractions during the visit.
Separation of IL-4 Production from Th Cell Proliferation by an Altered T Cell Receptor Ligand (1991)

In the presence of antigen presenting cells, a murine T helper (Th) cell specific for murine hemoglobin (Hb) responded to its immunogenic peptide by both cytokine (interleukin-4) secretion and proliferation. An altered Hb peptide with a single amino acid substitution induced only cytokine secretion and did not induce proliferation. Interleukin-1 costimulated and restored the Th proliferative response to normal levels. The altered peptide also supported cognate T cell-B cell interactions indicative of T cell helper function. Thus, this result suggests that the T cell receptor has the capacity of differential signaling.
How a mutation in the gene encoding sigma 70 suppresses the defective heat shock response caused by a mutation in the gene encoding sigma 32 (1992)

In Escherichia coli, transcription of the heat shock genes is regulated by sigma 32, the alternative sigma factor directing RNA polymerase to heat shock promoters. sigma 32, encoded by rpoH (htpR), is normally present in limiting amounts in cells. Upon temperature upshift, the amount of sigma 32 transiently increases, resulting in the transient increase in transcription of the heat shock genes known as the heat shock response. Strains carrying the rpoH165 nonsense mutation and supC(Ts), a temperature-sensitive suppressor tRNA, do not exhibit a heat shock response. This defect is suppressed by rpoD800, a mutation in the gene encoding sigma 70. We have determined the mechanism of suppression. In contrast to wild-type strains, the level of sigma 32 and the level of transcription of heat shock genes remain relatively constant in an rpoH165 rpoD800 strain after a temperature upshift. Instead, the heat shock response in this strain results from an approximately fivefold decrease in the cellular transcription carried out by the RNA polymerase holoenzyme containing mutant RpoD800 sigma 70 coupled with an overall increase in the translational efficiency of all mRNA species.
Fabrication technologies for three-dimensional integrated circuits (2003)

The MIT approach to 3D VLSI integration is based on low-temperature Cu-Cu wafer bonding. Device wafers are bonded in a face-to-back manner, with short vertical vias and Cu-Cu pads as the inter-wafer throughway. In our scheme, there are several reliability criteria, which include: (a) structural integrity of the Cu-Cu bond; (b) Cu-Cu contact electrical characteristics; and (c) process flow efficiency and repeatability. In addition, CAD tools are needed to aid in design and layout of 3DICs. This paper discusses recent results in all these areas.
Inhibition of browning by sulfur amino acids. 1. Heated amino acid-glucose systems (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInhibition of browning by sulfur amino acids. 1. Heated amino acid-glucose systemsMendel Friedman and Ibolya Molnar-PerlCite this: J. Agric. Food Chem. 1990, 38, 8, 1642–1647Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August 1990https://doi.org/10.1021/jf00098a004RIGHTS & PERMISSIONSArticle Views611Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (650 KB) Get e-Alerts Get e-Alerts