gebhart/patrepeval huggingface dataset:
Emission properties of a-plane GaN grown by metal-organic chemical-vapor deposition (2005)

We report on the emission properties of nonpolar a-plane GaN layers grown on r-plane sapphire. Temperature-, excitation-density-, and polarization-dependent photoluminescences and spatially resolved microphotoluminescence and cathodoluminescence are employed in order to clarify the nature of the different emission bands in the 3.0–3.5eV spectral range. In the near band-edge region the emission lines of the donor-bound excitons (3.472eV) and free excitons (3.478eV) are resolved in the polarized low-temperature spectra, indicating a good quality of the layers. At low energies two other emissions bands with intensity and shape varying with the excited area are observed. The 3.42eV emission commonly attributed to the excitons bound to basal plane stacking faults shows thermal quenching with two activation energies (7 and 30meV) and an S-shaped temperature dependence of the peak position. This behavior is analyzed in terms of hole localization in the vicinity of the stacking faults. The emission band that peaked at 3.29eV is found to blueshift and saturate with increasing excitation intensity. The spatially resolved cathodoluminesence measurements show that the emission is asymmetrically distributed around the triangular-shaped pits occurring at the surface. The 3.29eV emission is suggested to involve impurities, which decorate the partial dislocation terminating the basal stacking faults.
Adenovirus-Mediated Expression of the Secreted Form of Basic Fibroblast Growth Factor (FGF-2) Induces Cellular Proliferation and Angiogenesis In Vivo (1997)

Blood supply through collateral arteries is of critical importance in occlusive arterial diseases such as coronary atherosclerosis. Induction of angiogenic growth factor within either the narrowing arteries or jeopardized myocardium may promote angiogenesis in vivo, leading to salvage of ischemic myocardium. We constructed a replication-defective adenovirus (AdCAsFGF-2) coding for human basic fibroblast growth factor (FGF)-2 that is modified, so that its secretion will be facilitated, by tagging a signal sequence derived from FGF-4. A large quantity of FGF-2 was detected in both the cell lysate and culture medium of COS cells infected with AdCAsFGF-2, indicating that FGF-2 was secreted at least partly from the infected cells. The conditioned medium from the infected COS cells stimulated DNA synthesis in and induced cellular proliferation of arterial smooth muscle cells. These effects were eliminated by adenovirus-mediated overexpression of a dominant-negative truncated FGF-receptor type 1. Implantation of a gel of basement membrane proteins containing fibroblasts infected with AdCAsFGF-2 into the ventral subcutaneous space of mice induced extensive cellular proliferation and the formation of functional arterioles. Cells surrounding the vessels were positively immunostained with antibodies recognizing either smooth muscle-specific alpha-actin or factor VIII antigen as a marker for endothelium. These results suggest that AdCAsFGF-2 may be useful for delivering functional FGF-2 into tissues and may lead to therapeutic angiogenesis in vivo.
Cell shape and total adenylate concentration as important factors for posttransfusion survival of erythrocytes. (1983)

Red cells were stored for 42 days in a CPD/SAGM plastic bag system and then rejuvenated with bicarbonate, pyruvate and slow addition of adenosine. The reversibility and the time dependence of metabolic and morphologic changes were compared with autologous, posttransfusion survival. Total adenylates correlated better (r = 0.88) than ATP with viability in vivo ATP was better maintained when the blood was stored under anaerobic than aerobic conditions. A high correlation between morphology and viability was found in rejuvenated cells (r = 0.95). It is suggested that the time needed for reversal of changes is essential for viability.
Effect of diethylene glycol (DEG) on the crystallization behavior of poly(ethylene terephthalate) (PET) (1993)

Abstract The effect of diethylene glycol (DEG) on the crystallization of poly(ethylene terephthalate) (PET) was studied under isothermal and dynamic conditions. The strain‐induced crystallization of PET and its relationship to DEG content was also studied. The samples were isothermally and dynamically crystallized in the differential scanning calorimeter (DSC). The thermograms were then analyzed to determine the kinetic parameters. Strain‐induced crystallization was studied by stretching samples at different strain rates. These samples were then annealed for various periods of time and quenched to room temperature. Birefringence and density were measured on the annealed samples. Results indicate that the DEG content reduces the rate of crystallization of PET when crystallizing from the melt, isothermally and dynamically. When crystallizing from the glassy state, the effects of DEG are not prominent. The mechanism of crystallization is not affected by the amount of DEG, within the range of DEG contents evaluated. In the case of strain‐induced crystallization, increased DEG content reduces the crystallinity of PET at intermediate strain rates, but at higher strain rates, the crystallinity is not affected by the DEG content. © 1993 John Wiley &amp; Sons, Inc.
Using compound electrodes in electrical impedance tomography (1993)

In electrical impedance tomography, we inject currents and measure voltages to estimate an object's resistivity distribution. The electrode configuration affects measured voltage data because the electrode-skin contact impedance is high and varies with electrode location. We developed a compound electrode which is composed of two electrodes: a large outer electrode to inject current and a small inner electrode to sense voltage. We used these compound electrodes to measure voltages from a physical phantom. We showed that the measured voltages from the compound electrodes are smaller in amplitude than those from conventional electrodes. This demonstrates that the compound electrode can minimize contact impedance voltage drop from the measured data. We used a finite element model for the compound electrode and incorporated the model into the regularized Newton-Raphson reconstruction algorithm. We performed a sensitivity study and showed that the reconstructed resistivity distributions are less dependent on the unknown contact resistance values for a compound electrode than a conventional electrode and that the use of a compound electrode results in improved images for the reconstruction algorithm.
Alterations of NAD and adenylyl dinucleotide metabolism in Chediak‐Higashi Syndrome fibroblasts (1987)

Chediak-Higashi syndrome (CHS) cells have been previously observed to exhibit several of the same characteristics as those of xeroderma pigmentosum (XP) and xeroderma pigmentosum variants. Cultured CHS fibroblasts have been examined for altered responses in both depletion of NAD and elevation of diadenosine-5',5"'-tetraphosphate (Ap4A) following DNA damage since both responses have been reported as altered in XP cells and since Ap4A has been reported as absent from the platelets of CHS patients. Lowering of NAD following UV irradiation occurred in CHS cells in a manner similar to that of control and XP variant cells, but different from that of XP cells. CHS fibroblasts were not found to be deficient in Ap4A and exhibit basal levels very similar to those of control fibroblasts. No change in Ap4A pools were observed which correlated with cell growth, in contrast to previously published reports. Furthermore, while Ap4A levels are not elevated in XP cells, we observe an elevation of Ap4A pools in CHS cells which mimics the elevations observed in control and XP variant cells. We conclude that: (1) CHS cells more closely resemble control or XP variant cells than XP cells with regard to NAD lowering and Ap4A elevation following UV irradiation; (2) the photosensitivity exhibited by CHS cells is not due to general defects in the synthesis of poly(ADP-ribose) from NAD or in Ap4A metabolism; and (3) alteration of Ap4A pool size in CHS fibroblasts is inappropriate as a biochemical marker for CHS.
II. Diffusion of zinc and oxygen in zinc oxide (1959)

W. J. Moore and E. L. Williams, Discuss. Faraday Soc., 1959, 28, 86 DOI: 10.1039/DF9592800086
Targeting Cells with MR Imaging Probes Based on Paramagnetic Gd(III) Chelates (2004)

The low sensitivity is the major disadvantage of MRI as compared to PET. Therefore, amplification strategies are necessary for specific pathway labeling. This survey is aimed at exploring different routes to the entrapment of Gd(III) chelates in various type of cells at amounts sufficiently large to allow MRI visualization. Namely, the obtained results have been summarized in terms of internalization via i) pinocytosis; ii) phagocytosis; iii) receptors; iv) receptor mediated endocytosis; v) transporters; vi) transmembrane carrier peptides. MRI visualization of cells appears possible when the number of internalized Gd(III) chelates is of the order of 10(7)-10(8)/cell. Pinocytosis shows to be particularly useful for labeling cells that can be incubated for several hours in the presence of high concentrations of Gd-agent. This approach appears very effective for labeling stem cells. Nanoparticles filled with Gd-chelates can be used for an efficient loading of cells endowed with a good phagocytic activity. Entrapment via receptors most often results in receptor mediated endocytosis. Suitably functionalized monomeric and multimeric Gd-chelates can be considered for being internalized by this route as well as supramolecular systems such as those formed between Avidin and biotinylated Gd-complexes. Exploitation of up-regulated transporters of nutrients in tumor cells appears to be a promising route for their differentiation from healthy cells. Finally, properly designed systems entering the cells by means of penetrin-like peptides deserve great attention.
Cloud Computing and SOA Convergence Research (2012)

Cloud computing is Internet-based computing, and it takes every IT resource in the internet as a service to users, such as hardware services, infrastructure services, platform services, software services, storage services and so on. While SOA as a service oriented architecture, it's a software designing model and method. SOA can integrate well-defined services to a new solution. So, cloud computing and SOA has natural relationship, and they supply each other. This paper analyzes the basic theory of cloud computing and SOA firstly, and discusses the deployment and logic structure of cloud computing. Furthermore, the approaches combined with SOA to integrate cloud services are proposed. and the integration methods of SaaS and PaaS by adopting SOA are introduced respectively and detailedly.
A Case for Secure Virtual Append-Only Storage for Virtual Machines (2010)

Traditional operating systems and applications use logs extensively to monitor system activity and perform intrusion detection. Consequently, logs have also become prime targets for intruders. When a malware or intruder obtains root privileges in a system, one of its first actions is to hide its footprint by deleting or modifying system logs, especially the log entry recording the intrusion activity (such as unauthorized root login). A key weakness of most current logging mechanisms is that logs are stored on a storage device over which the system being logged has complete control, including the ability to delete/modify the logs arbitrarily. Once the root privileges of such a system are compromised, so are the logs. Virtualization offers a unique opportunity to eliminate this point of weakness. In this paper, we propose a new virtual storage abstraction for virtual machines (VMs) called Virtual Append-only Storage (VAS) that secures and preserves all system and/or application logs in a VM and can prevent an intruder from deleting/modifying past logs even after the root privileges of a VM are compromised. Our VAS-based logging complements existing intrusion detection techniques which mainly monitor the in-memory execution state and data, but do not protect the storage device on which logs are stored. Since logs can become voluminous over time, VAS also provides administrators the ability to secure either system-wide or application-specific logs, rather than blindly logging all system activity.
Kinetically controlled, adhesiveless transfer printing using microstructured stamps (2009)

This letter describes the physics and application of an approach to transfer printing that uses stamps with microstructures of relief embossed into their surfaces. Experimental measurement of velocity-dependent adhesive strength as a function of relief geometry reveals key scaling properties and provides a means for comparison to theoretical expectation. Formation of transistor devices that use nanoribbons of silicon transfer printed directly onto glass substrates without adhesive layers demonstrates the use of this type of approach for a high-performance (mobilities &amp;gt;325 cm2/V s and on/off ratios &amp;gt;105) single crystal silicon on glass technology.
Novel Mediators of FTY720 in Human Lymphocytes (2005)

FTY720 (FTY), a novel immunosuppressive drug, can be distinguished from other immunosuppressive drugs by a completely different mechanism of action. FTY induces altered lymphocyte trafficking, leading to peripheral blood lymphopenia and to increased lymphocyte counts in lymph nodes. FTY mediates its immune-modulating effects by binding to sphingosine 1-phosphate receptors expressed on lymphocytes. In an attempt to identify mediators of the FTY-induced signal transduction, we used a proteomic approach. FTY-treated peripheral blood lymphocytes (PBLs) were investigated for the expression of 622 proteins. We identified 15 differentially expressed proteins in PBLs possibly related to FTY action. As indicated by protein function, several identified proteins could be linked to the cytoskeleton/cell motility, to cell adhesion, and vesicle trafficking. No changes were found concerning the expression of various apoptosis regulators as well as the immunophilins FKBP12 and calcineurin. Our data suggest that FTY affects cytoskeleton rearrangements, cell adhesion, and vesicle trafficking/sorting in human PBLs.
Adaptive classification of two‐dimensional gel electrophoretic spot patterns by neural networks and cluster analysis (1997)

The interpretation of two-dimensional gel electrophoresis spot profiles can be facilitated by statistical and machine learning programs. Two different approaches to classification of spot profiles - cluster analysis and neural networks - are discussed. Neural networks for two different model patterns were designed and an algorithm for training of the net for the classification was developed. It was shown that the performance of neural networks is higher compared to cluster and principal component analysis. The possibility of combining both approaches into one process can increase reliability and speed of classification. Artificially created training sets with added random noise can be used for network training. The analysis was applied on the Streptomyces coelicolor developmental two-dimensional (2-D) gel database.
Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes (2013)

Alzheimer's disease (AD) is characterized by the accumulation of β-amyloid peptide (Aβ) in the brain because of an imbalance between Aβ production and clearance. Neprilysin (NEP) is the most important Aβ-degrading enzyme in the brain. Thus, researchers have explored virus-mediated NEP gene delivery. However, such strategies may entail unexpected risks, and thus exploration of a new possibility for NEP delivery is also required. Here, we show that human adipose tissue-derived mesenchymal stem cells (ADSCs) secrete exosomes carrying enzymatically active NEP. The NEP-specific activity level of 1 μg protein from ADSC-derived exosomes was equivalent to that of ~ 0.3 ng of recombinant human NEP. Of note, ADSC-derived exosomes were transferred into N2a cells, and were suggested to decrease both secreted and intracellular Aβ levels in the N2a cells. Importantly, these characteristics were more pronounced in ADSCs than bone marrow-derived mesenchymal stem cells, suggesting the therapeutic relevance of ADSC-derived exosomes for AD.
Monitoring CML patients responding to treatment with tyrosine kinase inhibitors: review and recommendations for harmonizing current methodology for detecting BCR-ABL transcripts and kinase domain mutations and for expressing results (2006)

The introduction in 1998 of imatinib mesylate (IM) revolutionized management of patients with chronic myeloid leukemia (CML) and the second generation of tyrosine kinase inhibitors may prove superior to IM. Real-time quantitative polymerase chain reaction (RQ-PCR) provides an accurate measure of the total leukemiacell mass and the degree to which BCR-ABL transcripts are reduced by therapy correlates with progression-free survival. Because a rising level of BCR-ABL is an early indication of loss of response and thus the need to reassess therapeutic strategy, regular molecular monitoring of individual patients is clearly desirable. Here we summarize the results of a consensus meeting that took place at the National Institutes of Health (NIH) in Bethesda in October 2005. We make suggestions for (1) harmonizing the differing methodologies for measuring BCR-ABL transcripts in patients with CML undergoing treatment and using a conversion factor whereby individual laboratories can express BCR-ABL transcript levels on an internationally agreed scale; (2) using serial RQ-PCR results rather than bone marrow cytogenetics or fluorescence in situ hybridization (FISH) for the BCR-ABL gene to monitor individual patients responding to treatment; and (3) detecting and reporting Philadelphia (Ph) chromosome-positive subpopulations bearing BCR-ABL kinase domain mutations. We recognize that our recommendations are provisional and will require revision as new evidence emerges.
Expression of multiple cancer-testis antigen genes in gastrointestinal and breast carcinomas (2001)

Cancer-testis antigens (CTAs) such as MAGE are selectively expressed in various types of human neoplasms but not in normal tissues other than testis. This characteristic feature of CTAs makes them promising antigens for cancer-specific immunotherapy. A critical requirement for this therapy is identification of promising antigens. In this study, we investigated the expression of 6 genes recently identified by serological analysis of antigens by recombinant expression (SEREX) libraries: NY-ESO-1, LAGE-1, SCP-1, SSX-1, SSX-2, and SSX-4, in many surgical samples of gastrointestinal and breast carcinomas using reverse transcription-polymerase chain reaction. We found relatively high expression of SCP-1 (23.5%) and SSX-4 (20.6%) in gastric carcinoma, LAGE-1 (39.1%) and NY-ESO-1 (23.9%) in oesophageal carcinoma, and SCP-1 (34.1%) in breast carcinoma. We also found frequent synchronous expression with MAGE, including LAGE-1 (46.2%) in oesophageal carcinoma, SSX-4 (46.7%) in gastric carcinoma, and SCP-1 (38.3%) in breast carcinoma. Immunohistochemical analysis of the tumour samples expressing both MAGE-4 and NY-ESO-1 genes demonstrated differences in distribution between MAGE-4 and NY-ESO-1 in serial sections. We concluded that NY-ESO-1, LAGE-1, SCP-1 and SSX-4 genes may be promising candidates for cancer-specific immunotherapy in addition to MAGE, and that polyvalent cancer vaccines may be useful in cases of heterogeneous expressions of CTA genes in gastrointestinal and breast carcinomas.
Complement C6 Deficiency Protects Against Diet-Induced Atherosclerosis in Rabbits (1998)

Low-density lipoprotein (LDL) can be transformed to an atherogenic moiety by nonoxidative, enzymatic degradation. Enzymatically degraded LDL induces macrophage foam cell formation, provokes release of cytokines, and also activates complement. To determine whether complement activation may contribute to atherogenesis, 6 pairs of homozygous C6-deficient rabbits and their non-C6-deficient heterozygous siblings were fed a cholesterol-rich diet for 14 weeks. Cholesterol levels and plasma lipoprotein profiles of the animals in the C6-competent and C6-deficient groups did not significantly differ, and the high density lipoprotein and LDL cholesterol ratios at the end of the experiment were 0.07+/-0.01 and 0.08+/-0.01 (SEM), respectively. However, differences in atherosclerotic plaque formation were discernible macroscopically, with extensive aortic lesions being visible in all C6-competent animals and absent in all C6-deficient animals. Aortas were sectioned from thorax to abdomen, and 10 sections were stained from each aorta. Quantification of atherosclerotic lesions and lumen stenosis with the use of computer-based morphometry documented a dramatic protective effect of C6 deficiency on the development of diet-induced atherosclerosis. We conclude that the terminal complement sequence is centrally involved in atherosclerotic lesion progression.
A call for transparent reporting to optimize the predictive value of preclinical research (2012)

The US National Institute of Neurological Disorders and Stroke convened major stakeholders in June 2012 to discuss how to improve the methodological reporting of animal studies in grant applications and publications. The main workshop recommendation is that at a minimum studies should report on sample-size estimation, whether and how animals were randomized, whether investigators were blind to the treatment, and the handling of data. We recognize that achieving a meaningful improvement in the quality of reporting will require a concerted effort by investigators, reviewers, funding agencies and journal editors. Requiring better reporting of animal studies will raise awareness of the importance of rigorous study design to accelerate scientific progress.
Dislocation reduction via selective-area growth of InN accompanied by lateral growth by rf-plasma-assisted molecular-beam epitaxy (2010)

We investigated the selective-area growth (SAG) of InN by rf-plasma-assisted molecular-beam epitaxy using molybdenum (Mo)-mask-patterned sapphire (0001) substrates, which resulted in the formation of regularly arranged N-polar InN microcrystals. Transmission electron microscopy observation confirmed that the laterally grown side areas were nearly dislocation-free, although many threading dislocations (109–1010 cm−2) were generated at the InN/sapphire interface and propagated into the center of the InN microcrystals along the crystal c-axis. The laterally grown InN microcrystals exhibited narrow near-IR emission spectra with a peak photon energy of 0.627 eV and a linewidth of 39 meV at room temperature.
Interleukin-1 Blockade Attenuates Mediator Release and Dysregulation of the Hemostatic Mechanism During Human Sepsis (1995)

The results confirm that activation of the coagulation and fibrinolytic systems and release of soluble inflammatory mediators are consistently observed in patients with severe sepsis syndrome. Interleukin-1 activity contributes to activation of these processes as documented by the reduction in surrogate activation markers during recombinant human interleukin-1 receptor antagonist treatment.