gebhart/patrepeval huggingface dataset:
Concise Synthesis of 1H-Pyrazin-2-ones and 2-Aminopyrazines (2004)

Convenient syntheses of 1H-pyrazin-2-ones and 2-aminopyrazines are described. By coupling Boc-protected amino acids with α-amino ketones or with amino alcohols and subsequent oxidation, 1H-pyrazin-2-ones were obtained. Transformation into the corresponding pyrazine triflates and substitution with primary or secondary amines led to 2-aminopyrazines. Since these syntheses take advantage of the use of readily available starting materials (e.g., amino acids, aminoalcohols and amines) a variety of the entitled structures can be obtained in few, high yielding steps.
Recombinant Mouse OB Protein: Evidence for a Peripheral Signal Linking Adiposity and Central Neural Networks (1995)

The recent positional cloning of the mouse ob gene and its human homology has provided the basis to investigate the potential role of the ob gene product in body weight regulation. A biologically active form of recombinant mouse OB protein was overexpressed and purified to near homogeneity from a bacterial expression system. Peripheral and central administration of microgram doses of OB protein reduced food intake and body weight of ob/ob and diet-induced obese mice but not in db/db obese mice. The behavioral effects after brain administration suggest that OB protein can act directly on neuronal networks that control feeding and energy balance.
Production and characterization of murine models of classic and intermediate maple syrup urine disease (2006)

These mice represent important animal models that closely approximate the phenotype of humans with the classic and intermediate forms of MSUD. These animals provide useful models to further characterize the pathogenesis of MSUD, as well as models to test novel therapeutic strategies, such as gene and cellular therapies, to treat this devastating metabolic disease.
Imiquimod (2003)

In the future, imiquimod and newer generations of imidazoquinolines (resiquimod) require further investigation for potential clinical utility in treating other cutaneous and mucosal viral infections, dysplasias and neoplasia, as well as potential vaccine adjuvants.
Chronic distress and incidence of mild cognitive impairment (2007)

Among older persons without manifest cognitive impairment, higher level of chronic psychological distress is associated with increased incidence of mild cognitive impairment.
Nitrile Reduction in the Presence of Boc-Protected Amino Groups by Catalytic Hydrogenation over Palladium-Activated Raney-Nickel (2001)

Polyamines have attracted considerable interest, especially after the discovery of their ubiquitous presence in living cells and their involvement in many metabolic processes.1 As part of a program to synthesize homologues and derivatives of naturally occurring polyamines, we utilized the reduction of nitriles in the presence of Boc-protected amino groups. Nitriles are a versatile synthon for amines in organic chemistry and can be prepared by a variety of methods.2 Furthermore, there are a number of methods available for the subsequent reduction to amines.2 Catalytic hydrogenation3 has been carried out using Raney-nickel,4,5 Pd(OH)2/C,6 Pd/C,7 or PtO28 as catalyst. Alternatively, complex metal hydrides,9 especially NaBH4 + CoCl2·6H2O,10 NaBH4 + NiCl2·6H2O,11 and LiAlH4 variations,12 have been used successfully to achieve the nitrile-amine transformation. Here we report the use of palladium activated Raney-nickel for the reduction of nitriles to primary amines for cases in which other methods were unsuccessful.
RNA-Directed Transcriptional Gene Silencing and Activation in Human Cells (2009)

The overt loss or uncontrolled gain of gene expression is found at some level in virtually every malady afflicting humans. From cancer to HIV-1, the uncontrolled expression or loss of gene expression is prevalent in human diseases. Approaches toward the specific control of gene expression at the transcriptional level could have the potential to revert or reduce disease pathologies. Over the last several years, researchers have developed methodologies that utilize small antisense non-coding RNAs to specifically silence transcription. Only recently has the endogenous molecular pathway usurped by the introduction of these small RNAs to regulate transcription in human cells been defined. Observations suggest that long antisense non-coding RNAs function as the endogenous epigenetic regulators of transcription in human cells, thus explaining why small antisense RNAs were observed early on to silence transcription via directed epigenetic changes at the target loci. The mechanism of action whereby small regulatory RNAs can either turn gene transcription on or off will be discussed as evidence that one day it may be possible to develop therapeutics to regulate gene transcription and ameliorate particular disease conditions.
Minimally Infrequent Itemset Mining using Pattern-Growth Paradigm and Residual Trees (2012)

Itemset mining has been an active area of research due to its successful application in various data mining scenarios including finding association rules. Though most of the past work has been on finding frequent itemsets, infrequent itemset mining has demonstrated its utility in web mining, bioinformatics and other fields. In this paper, we propose a new algorithm based on the pattern-growth paradigm to find minimally infrequent itemsets. A minimally infrequent itemset has no subset which is also infrequent. We also introduce the novel concept of residual trees. We further utilize the residual trees to mine multiple level minimum support itemsets where different thresholds are used for finding frequent itemsets for different lengths of the itemset. Finally, we analyze the behavior of our algorithm with respect to different parameters and show through experiments that it outperforms the competing ones.
Correlation of estrogen and progesterone receptors with histologic differentiation in mammary carcinoma (1980)

Using a modification of the histologic grading system of the NSABP, we observed a trend towards higher levels of estrogen (E2R) and progesterone receptor (PR) content in well (grade I) and moderately (grade II) differentiated mammary carcinomas. This relationship between receptor content and histologic grade is enhanced by considering estrogen and progesterone receptor simultaneously. The rank correlation between the quantitative levels of E2R and PR was 0.74 among histologic grade I tumors and 0.64 among histologic grade II tumors. Among the grade III carcinomas, the majority of tumors displayed either a paucity of measurable receptor or a divergence between levels of estrogen versus progesterone receptor (r = 0.19). The use of ultrastructural evaluation of features of differentiation is discussed in the evaluation of grade III tumors and in the evaluation of specific histologic types of mammary carcinoma.
Host-graft circulation and vascular morphology in pancreatic tissue transplants in rats (1998)

One of the most determining factors for the survival of tissue grafts is an intact vasculature. This vasculature must, however, be linked to the circulation of the host animals for survival and growth to occur. The mechanism(s) of revascularization of pancreatic tissue grafts is still unclear and more so the process by which the host blood vessels anastomose with those of the graft. The microvasculature and revascularization of neonatal pancreatic tissue fragments transplanted into the anterior eye chamber of rats were investigated using conventional light and electron microscopy as well as the india ink perfusion method. Light microscopy demonstrated that the blood vessels of the host iris revascularized the transplants within 24 h of transplantation. Sinusoidal blood capillaries were observed to invade the peripheral parts of the grafts. The capillary encroachment from the iris into the graft continued through the second day of transplantation. The host-graft anastomosis developed completely and became prominent and conspicuous after the third day of transplantation. Many capillaries were observed to encroach into the graft from the iris. The vasculature of the graft was increased in comparison with that of normal pancreatic tissue. This increase was prominent especially around pancreatic islet and ductal cells which survived after transplantation. Blood cells observed in these vessels of the graft indicated a functional state. The ultrastructure of the intrinsic blood vessels of the graft was intact and showed the peculiar fenestrations normally seen in the blood vessels of endocrine tissues. These observations indicated that pancreatic tissue fragments transplanted into the anterior eye chamber of rats were revascularized within 24 h. The revascularization was completed at the end of the third day of transplantation when definitive blood vessels linked the circulation of the graft with that of the iris.
Colorimetric Method for Determination of Sugars and Related Substances (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTColorimetric Method for Determination of Sugars and Related SubstancesMichel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, and Fred. SmithCite this: Anal. Chem. 1956, 28, 3, 350–356Publication Date (Print):March 1, 1956Publication History Published online1 May 2002Published inissue 1 March 1956https://pubs.acs.org/doi/10.1021/ac60111a017https://doi.org/10.1021/ac60111a017research-articleACS PublicationsRequest reuse permissionsArticle Views60836Altmetric-Citations37027LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Thrombin activatable fibrinolysis inhibitor and its fibrinolytic effect in normal pregnancy (2004)

We investigated changes in both thrombin activatable fibrinolysis inhibitor (TAFI) antigen levels and its functional effect on in vitro fibrinolysis in normal pregnancy. 152 pregnant women and 31 women in the immediate postpartum period were studied, with pregnancy divided into 6 windows at 4 weekly intervals. As TAFI influences and is in turn influenced by components of the protein C (PC) pathway, its measurements were correlated with levels of soluble thrombomodulin, PC, protein S (PS) and the overall phenotype of activated PC resistance (APCR). Compared with mean TAFI levels at booking gestation (6.6 +/- 1.2 microg/ml), levels peaked at 35-39 weeks gestation (9.6 +/- 2 microg/ml, p = 0.001), followed by a significant drop within 24 hours of delivery (7.2 +/- 1.1 microg/ml). In functional terms, the mean clot lysis time (CLT) (101 +/- 13 min at booking) also peaked at 35-39 weeks gestation (141 +/- 42 min, p = 0.007) and dropped after delivery (99 +/- 33 min), and was significantly correlated with gestational age (r = 0.410, p = 0.001) and could be abrogated in the presence of an inhibitor to TAFI activation. A significant negative correlation was found between TAFI levels and APCR (r = -0.478, p <0.001), APCRV (r = -0.598; p <0.001), PS (r = -0.490, P <0.001) and PC (r = -0.198, p = 0.02). In summary, there is a significant increase in TAFI levels, which translates into increased CLT during pregnancy. Furthermore, changes in TAFI contribute to the increasing APCR of pregnancy.
Microbial Electrolysis Cells for High Yield Hydrogen Gas Production from Organic Matter (2008)

The use of electrochemically active bacteria to break down organic matter, combined with the addition of a small voltage (> 0.2 V in practice) in specially designed microbial electrolysis cells (MECs), can result in a high yield of hydrogen gas. While microbial electrolysis was invented only a few years ago, rapid developments have led to hydrogen yields approaching 100%, energy yields based on electrical energy input many times greater than that possible by water electrolysis, and increased gas production rates. MECs used to make hydrogen gas are similar in design to microbial fuel cells (MFCs) that produce electricity, but there are important differences in architecture and analytical methods used to evaluate performance. We review here the materials, architectures, performance, and energy efficiencies of these MEC systems that show promise as a method for renewable and sustainable energy production, and wastewater treatment.
Class IIa Bacteriocins: Diversity and New Developments (2012)

Class IIa bacteriocins are heat-stable, unmodified peptides with a conserved amino acids sequence YGNGV on their N-terminal domains, and have received much attention due to their generally recognized as safe (GRAS) status, their high biological activity, and their excellent heat stability. They are promising and attractive agents that could function as biopreservatives in the food industry. This review summarizes the new developments in the area of class IIa bacteriocins and aims to provide uptodate information that can be used in designing future research.
Fetal fraction in maternal plasma cell‐free DNA at 11–13 weeks' gestation: relation to maternal and fetal characteristics (2012)

Fetal fraction in maternal plasma cf-DNA is affected by maternal and fetal characteristics.
Initiation and maintenance of multiple myeloma. (1999)

A recently identified herpesvirus, human herpesvirus-8 (HHV-8), also known as Kaposi's sarcoma (KS)-associated herpesvirus, has been found in nonmalignant bone marrow dendritic cells of patients with multiple myeloma. The virus is also detectable in the peripheral blood of most patients; its absence suggests earlier-stage disease. HHV-8 is not detected in the blood of family members and sexual partners of myeloma patients. Sequencing of HHV-8 open-reading frames (ORFs) shows differences between individual myeloma patients, as well as between myeloma patients and those with other HHV-8-related malignancies. Consistent expression of the viral homolog of both interferon regulatory factor (IRF) and interleukin-8 receptor (IL-8R) suggests a possible role for these transforming viral genes in the pathogenesis of myeloma. Detailed analysis of myeloma cell lines has shown that myeloma is characterized by frequent chromosome translocations involving the switch regions of the immunoglobulin heavy-chain (IgH) locus on 14q32. The three partner chromosomes most commonly involved are 11q13 (cyclin D1), 4p16 (FGFR3), and 16q23 (c-maf). Mutations have also been identified in N- and K-ras and, less frequently, involving p53. Monosomy 13q is common. These findings have implications for immunotherapy. Angiogenesis increases with progressive disease and appears to be a prognostic factor. In at least one patient, this process appears to have been reversed with thalidomide therapy. The underlying mechanisms for the increased vascularization in myeloma have not been identified, and several possibilities have been proposed.
Humoral immunotherapy of multiple myeloma: perspectives and perplexities (2010)

mAbs directed against MM-associated adhesion markers should be taken into account in clinical practice, since they could possibly represent the best available combination of tumor cytotoxicity, environmental signal deprivation and immune system redirection.
Metabolism of 1,2-dimethylhydrazine by cultured human colon (1980)

The overall metabolism of 1,2-dimethylhydrazine, and organotropic colon carcinogen in rodents, has been studied using human colon explant cultures. The binding level of 1,2-dimethylhydrazine to DNA which in this study includes both reaction of metabolites with DNA and incorporation of radioactive metabolites into DNA, showed a 100-fold variation among the 120 people studied. When different anatomical colonic sites were compared, the highest mean binding levels were found in the ascending and sigmoid colon. No significant difference in the median and mean binding levels were observed in nontumorous colon obtained surgically from patients with colon cancer and colon obtained from immediate autopsy, but decreased mean binding levels were seen in tissues obtained by surgery from patients with non-cancerous colonic disorders. Several exogenous chemicals were found to modify the metabolism. When the colon explants were co-incubated with 1,2-dimethylhydrazine and these chemicals, the binding level of 1,2-dimethylhydrazine to DNA was (a) increased by either indole 3-carbinol or phenobarbital, (b) decreased with disulfiram, butylated hydroxytoluene, or taurodeoxycholic acid, and (c) unaltered by lithocholic acid.
Changes of cardiovascular function caused by short arm centrifuge exposure and aerobic exercise (2010)

Objective To investigate the changes of cardiovascular function and cardiovascular autonomic nervous regulation caused by short arm centrifuge exposure (SAC), aerobic exercise (EX),or their combination (SAC+ EX).Methods Twenty-four healthy male subjects were randomly and averagely divided into 3 groups that executed SAC, EX and SAC+ EX respectively.Subjects in SAC group experienced 1-2 G centrifuge training while EX group merely performed aerobic exercise with 40 W-load.In SAC+ EX group, subjects performed 40 W-load exercise for 30 min when they exposed on centrifuge once a day for 1 week.Electrocardiogram, continue blood pressure were monitored and used for HR variation and systolic blood pressure variation spectrum a(n)alysis.The training caused changes on systole and blood-pumping functions were got by analyzing mechanical-cardiogram and impedance-cardiogram.Results Analysis showed that 1-week SAC+EX training improved subjects' cardiac pumping function and cardiovascular autonomic nervous modulation, which associated with significant decreased heart rate (P＜0.05) and significant increased stroke volume (SV), left ventricular ejection time (LVET) and lower frequency (0.04-0.15 Hz) of arterial pressure spectral power (P＜0.05) as compared with those in other 2 groups.Conclusions 1-week SAC+40 W-load EX training could significantly improve cardiac pumping function, cardiac vagal modulation and peripheral smooth muscle vascular tone.Such combined action promotes the changes.However,alternative SAC or EX training is not sufficient enough to induce similar alterations.

Key words: 
Weightlessness countermeasure ;  Gravity,altered ;  Exercise ;  Heart function tests ;
The HSV-1 Exonuclease, UL12, Stimulates Recombination by a Single Strand Annealing Mechanism (2012)

Production of concatemeric DNA is an essential step during HSV infection, as the packaging machinery must recognize longer-than-unit-length concatemers; however, the mechanism by which they are formed is poorly understood. Although it has been proposed that the viral genome circularizes and rolling circle replication leads to the formation of concatemers, several lines of evidence suggest that HSV DNA replication involves recombination-dependent replication reminiscent of bacteriophages λ and T4. Similar to λ, HSV-1 encodes a 5'-to-3' exonuclease (UL12) and a single strand annealing protein [SSAP (ICP8)] that interact with each other and can perform strand exchange in vitro. By analogy with λ phage, HSV may utilize viral and/or cellular recombination proteins during DNA replication. At least four double strand break repair pathways are present in eukaryotic cells, and HSV-1 is known to manipulate several components of these pathways. Chromosomally integrated reporter assays were used to measure the repair of double strand breaks in HSV-infected cells. Single strand annealing (SSA) was increased in HSV-infected cells, while homologous recombination (HR), non-homologous end joining (NHEJ) and alternative non-homologous end joining (A-NHEJ) were decreased. The increase in SSA was abolished when cells were infected with a viral mutant lacking UL12. Moreover, expression of UL12 alone caused an increase in SSA, which was completely eliminated when a UL12 mutant lacking exonuclease activity was expressed. UL12-mediated stimulation of SSA was decreased in cells lacking the cellular SSAP, Rad52, and could be restored by coexpressing the viral SSAP, ICP8, indicating that an SSAP is also required. These results demonstrate that UL12 can specifically stimulate SSA and that either ICP8 or Rad52 can function as an SSAP. We suggest that SSA is the homology-mediated repair pathway utilized during HSV infection.
The distribution of colony‐forming cells among spleen colonies (1963)

Progenitor cells that are recognized by their ability to form colonies of descendants in the spleens of irradiated mice have the capacity for self-renewal. The distribution of new colony-forming cells per colony is extremely heterogeneous, indicating lax control of self-renewal. The capacities of colony-forming cells for self-renewal, for extensive proliferation, and for giving rise to differentiated descendants, fulfill three requirements for studies of stem cells. Thus, colony-forming cells may be considered to be class (though not necessarily the only class) of such progenitor cells, and the spleen colony method is a quantitative method for their detection. Journal of Cellular and Comparative Physiology copyright 1963 Wiley-Liss, Inc., A Wiley Company (www.interscience.Wiley.com).
Substrate Competitive Inhibitors of IGF-1 Receptor Kinase (2000)

IGF-1 and its receptor play a pivotal role in many cancers, and therefore, IGF-1R is an attractive target for the design of inhibitors. In this communication, we report on a number of lead compounds for inhibitors of the isolated IGF-1R kinase. The search for these compounds utilized two novel in vitro assays and was aided by the knowledge of the three-dimensional structure of the insulin receptor kinase domain, which is 84% homologous to the IGF-1R kinase domain. The most potent inhibitor found in these assays was tyrphostin AG 538, with an IC(50) = 400 nM. In computer modeling, AG 538 was placed in the kinase domain of the insulin receptor and was able to sit in place of tyrosines 1158 and 1162, which undergo autophosphorylation. Experimentally it is indeed found that AG 538 does not compete with ATP but competes with the IGF-1R substrate. We prepared I-OMe AG 538, which is more hydrophobic and less sensitive to oxidation than AG 538. Both AG 538 and I-OMe AG 538 inhibit IGR-1R autophosphorylation in intact cells in a dose-dependent manner but I-OMe-AG 538 is superior, probably because of its enhanced hydrophobic nature. Both compounds inhibit the activation of the downstream targets PKB and Erk2. These findings suggest that AG 538 and I-OMe-AG 538 can serve as a lead compound for the development of substrate competitive inhibitors of the IGF-1R. The possible advantage of substrate competitive inhibitors vis-à-vis ATP competitive inhibitors is discussed.