gebhart/patrepeval huggingface dataset:
Facile Synthesis of Gold−Silver Nanocages with Controllable Pores on the Surface (2006)

Gold-silver alloy nanocages with controllable pores on the surface have been synthesized via galvanic replacement reaction between truncated Ag nanocubes and aqueous HAuCl4. Unlike in the previous studies, the initiation of replacement reaction started in a controllable way, simultaneously from eight corners of the truncated Ag nanocubes where {111} facets were exposed. The formation of cubic nanocages with pores at all the corners was determined by the capping agent, poly(vinyl pyrrolidone) (PVP), which preferentially covered the {100} facets of a truncated Ag nanocube.
Intramolecular electron transfer mediated by 4,4'-bipyridine and related bridging groups (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntramolecular electron transfer mediated by 4,4'-bipyridine and related bridging groupsH. Fischer, G. M. Tom, and H. TaubeCite this: J. Am. Chem. Soc. 1976, 98, 18, 5512–5517Publication Date (Print):September 1, 1976Publication History Published online1 May 2002Published inissue 1 September 1976https://pubs.acs.org/doi/10.1021/ja00434a018https://doi.org/10.1021/ja00434a018research-articleACS PublicationsRequest reuse permissionsArticle Views459Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Scalability of Roll-to-Roll Gravure-Printed Electrodes on Plastic Foils (2010)

Roll-to-roll (R2R) gravure printing is considered to be a leading technology for the production of flexible and low-cost printed electronics in the near future. To enable the use of R2R gravure in printed electronics, the limits of overlay printing registration accuracy (OPRA) and the scalability of printed features with respect to the physical parameters of the gravure system, including given plastic substrates and inks, should be characterized. Important parameters of printed lines include surface roughness, thickness, line widening, and line-edge roughness. To date, there are no comprehensive reports regarding the limits of OPRA and the scalability of printed electrodes, including the control of surface roughness, thickness, line widening, and line-edge roughness using R2R gravure printing. In this paper, we examine ways of evaluating the OPRA limit of our gravure system. We find that OPRA is limited in the web moving direction to 40 μm and in the perpendicular direction to 16 μm, showing the importance of web handling on registration. Furthermore, we demonstrate the scalability of printed electrodes formed using a R2R gravure system to linewidths of 317 μm, with 440 nm thickness, 30 nm of surface roughness and edge waviness of 4 μm on PET foils, and describe optimization strategies to realize improved surface roughness, thickness, line widening, and line-edge roughness for future printed electronics applications.
Experimental cerebral hemodynamics (1974)

✓ Application of Burton's concept of the critical closing pressure to experimental data on brain-blood flow in the monkey suggests that perfusion pressure, not vascular bed resistance, is the primary variable affecting cerebral blood flow. Perfusion pressure for the cerebral circulation is the mean arterial pressure minus the critical closing pressure (MAP — CCP). Vasomotor tone and intracranial pressure are the major determinants of the critical closing pressure. Changes in either of these variables, therefore, affect perfusion pressure and flow. Data on brain-blood flow at fixed vasomotor tone obtained over wide pressure ranges show little change in vascular bed resistance despite significant changes in flow. The diameter of resistance vessels probably does not change significantly throughout the normal physiological range of cerebral blood flow. The limits of the critical closing pressure in the anesthetized monkey are from 10 to 95 mm Hg. Using these limits, and beginning with the average values for MAP and CCP in 11 awake monkeys breathing room air, the authors present theoretical flow curves in response to changes in intracranial pressure and mean arterial pressure that closely approximate the data reported in man.
Estrogen response in the hFOB 1.19 human fetal osteoblastic cell line stably transfected with the human estrogen receptor gene (1995)

The gene coding for the human wild-type estrogen receptor (ER) was stably transfected into the human fetal osteoblastic cell line hFOB 1.19, a clonal cell line which is conditionally immortilized with a temperature sensitive mutant of SV40 large T antigen (tsA58). Five subclones were obtained which express various levels of ER mRNA and protein. The subclone with the highest level of functional (nuclear bound) ER, hFOB/ER9, contained 3,931 (+/- 1,341) 17beta-estradiol molecules bound/nucleus as determined by the nuclear binding (NB) assay. Using the dextran coated charcoal (DCC) method, the level of total cytosolic ER measured was 204 (+/- 2) fmol/mg protein. This subclone was examined further for estradiol (E2) responsiveness. The ER expressed in hFOB/ER9 cells was shown to be functional using a transiently transfected ERE-TK-luciferase construct. Expression of luciferase from this construct increased approximately 25-fold in hFOB/ER9 cells following 10(-9)M E2 treatment. This effect on ERE-TK-luciferase expression was both dose and steroid dependant. Further, treatment of hFOB/ER9 cells with 10(-9)M E2 resulted in a 2.5-4.0-fold increase in endogenous progesterone receptor (PR) levels detected by steroid binding assays, and a noticeable increase in both the A and B forms of PR by western blot assay. The establishment of this estrogen responsive human osteoblastic cell line should provide an excellent model system for the study of estrogen action on osteoblast function.
Link Between the Angiographic Substudy and Mortality Outcomes in a Large Randomized Trial of Myocardial Reperfusion (1995)

The close relation between the predicted and observed 30-day mortality rates supports the concept that an important mechanism for improved survival with thrombolytic therapy is achievement of early, complete perfusion. The close match provides a strong biological explanation for the mortality differences seen in GUSTO-I and a sound rationale for the additional survival advantage of the accelerated TPA regimen. Irrespective of which treatment is used, early and complete restoration of infarct artery perfusion represents an essential goal of myocardial reperfusion therapy.
Kinesin light chain -independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte (2002)

Microtubules and the Kinesin heavy chain, the force-generating component of the plus end-directed microtubule motor Kinesin I are required for the localisation of oskar mRNA to the posterior pole of theDrosophila oocyte, an essential step in the determination of the anteroposterior axis. We show that the Kinesin heavy chain is also required for the posterior localisation of Dynein, and for all cytoplasmic movements within the oocyte. Furthermore, the KHC localises transiently to the posterior pole in an oskar mRNA-independent manner. Surprisingly, cytoplasmic streaming still occurs in kinesin light chain null mutants, and bothoskar mRNA and Dynein localise to the posterior pole. Thus, the Kinesin heavy chain can function independently of the light chain in the oocyte, indicating that it associates with its cargoes by a novel mechanism.
Localization of the Functional Domains of Human Tissue Inhibitor of Metalloproteinases-3 and the Effects of a Sorsby's Fundus Dystrophy Mutation (1998)

A transient COS-7 cell expression system was used to investigate the functional domain arrangement of tissue inhibitor of metalloproteinases-3 (TIMP-3), specifically to assess the contribution of the amino- and carboxyl-terminal domains of the molecule to its matrix metalloproteinase (MMP) inhibitory and extracellular matrix (ECM) binding properties. Wild type TIMP-3 was entirely localized to the ECM in both its glycosylated (27 kDa) and unglycosylated (24 kDa) forms. A COOH-terminally truncated TIMP-3 molecule was found to be a non-ECM bound MMP inhibitor, whereas a chimeric TIMP molecule, consisting of the NH2-terminal domain of TIMP-2 fused to the COOH-terminal domain of TIMP-3, displayed ECM binding, albeit with a lower affinity than the wild type TIMP-3 molecule. Thus the functional domain arrangement of TIMP-3 is analogous to that seen in TIMP-1 and -2, namely that the NH2-terminal domain is responsible for MMP inhibition whereas the COOH-terminal domain is most important in mediating the specific functions of the molecule. A mutant TIMP-3 in which serine 181 was changed to a cysteine, found in Sorsby's fundus dystrophy, a hereditary macular degenerative disease, was also expressed in COS-7 cells. This gave rise to an additional 48-kDa species (possibly a TIMP-3 dimer) that retained its ability to inhibit MMPs and localize to the ECM. These data favor the hypothesis that the TIMP-3 mutations seen in Sorsby's fundus dystrophy contribute to disease progression by accumulation of mutant protein rather than by the loss of functional TIMP-3.
MetMAb, the One-Armed 5D5 Anti-c-Met Antibody, Inhibits Orthotopic Pancreatic Tumor Growth and Improves Survival (2008)

Abstract The hepatocyte growth factor (HGF) and its receptor, c-Met, have been implicated in driving proliferation, invasion, and poor prognosis in pancreatic cancer. Here, we investigated the expression of HGF and c-Met in primary pancreatic cancers and described in vitro and in vivo models in which MetMAb, a monovalent antibody against c-Met, was evaluated. First, expression of HGF and MET mRNA was analyzed in 59 primary pancreatic cancers and 51 normal samples, showing that both factors are highly expressed in pancreatic cancer. We next examined HGF responsiveness in pancreatic cancer lines to select lines that proliferate in response to HGF. Based on these studies, two lines were selected for further in vivo model development: BxPC-3 (c-Met+, HGF−) and KP4 (c-Met+, HGF+) cells. As BxPC-3 cells are responsive to exogenous HGF, s.c. tumor xenografts were grown in a paracrine manner with purified human HGF provided by osmotic pumps, wherein MetMAb treatment significantly inhibited tumor growth. KP4 cells are autocrine for HGF and c-Met, and MetMAb strongly inhibited s.c. tumor growth. To better model pancreatic cancer and to enable long-term survival studies, an orthotopic model of KP4 was established. MetMAb significantly inhibited orthotopic KP4 tumor growth in 4-week studies monitored by ultrasound and also improved survival in 90-day studies. MetMAb significantly reduced c-Met phosphorylation in orthotopic KP4 tumors with a concomitant decrease in Ki-67 staining. These data suggest that the HGF/c-Met axis plays an important role in the progression of pancreatic cancer and that targeting c-Met therein may have therapeutic value. [Cancer Res 2008;68(11):4360–8]
An operational MF broadcast antenna using Poynting vector synthesis (1991)

Describes a successful MF antenna which is only 1.6% of a wavelength high, and is in daily service at 60 kW for AM broadcasting. Poynting (1884) showed that wherever electrical energy is moving it flows as a field disturbance in the volume surrounding the conductors. The construction details of the crossed-field antenna are included together with the results of impedance, bandwidth, and field strength measurements. >
Collagen in the normal and hypertrophied human ventricle (1977)

Estimates of the collagen concentration in human ventricles have been made from measurements of the hydroxyproline concentration. In the normal heart the concentration of collagen was higher in the right ventricle than in the left ventricle. Age had no effect on the ventricular concentration of collagen. Hypertrophy in the absence of a valvar lesion was not associated with an increased concentration of collagen but, owing to the increased size of the ventricle, there was an increase in the estimated total mass of ventricular collagen. The concentration of collagen in the left ventricle of patients with aortic stenosis was higher than normal. Ventricular hypertrophy seems to be accompanied by an increase in the total mass of collagen whatever the cause. Whether the concentration changes or not depends on the proportion in which the mass of collagen increases relative to the mass of muscle cells.
Current Understanding and Management of Medullary Thyroid Cancer (2013)

Medullary thyroid cancer (MTC) typically accounts for 3%-4% of all thyroid cancers. Although the majority of MTCs are sporadic, 20% of cases are hereditary. Hereditary MTC can be found in multiple endocrine neoplasia 2A or 2B or as part of familial MTC based on a specific germline mutation in the RET proto-oncogene. This article discusses the current approaches available for the diagnosis, evaluation, and management of patients and their family members with suspected MTC. The disease is predominantly managed surgically and typically requires a total thyroidectomy and lymph node dissection. A review of recent guidelines on the extent and timing of surgical excision is discussed. There are not very many effective systemic treatment options for MTC, but several emerging therapeutic targets have promise.
Acylation—Alkylation Studies. I1 (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcylation—Alkylation Studies. I1Robert T. Hart and R. F. TebbeCite this: J. Am. Chem. Soc. 1950, 72, 7, 3286–3287Publication Date (Print):July 1, 1950Publication History Published online1 May 2002Published inissue 1 July 1950https://pubs.acs.org/doi/10.1021/ja01163a513https://doi.org/10.1021/ja01163a513research-articleACS PublicationsRequest reuse permissionsArticle Views475Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Activation of PI3K/AKT signaling pathway in malignant pleural mesothelioma cells (2008)

2721 Background:  The phosphatidylinositol 3 kinase (PI3K) /AKT signaling pathway is important for cell survival and proliferation, and it has a significant role in carcinogenesis. Deregulated activation of this pathway has been found in many human common malignancies, either by loss of PTEN phosphatase, a negative regulator of PI3K pathway, or by constitutive activation of PI3K or downstream elements including AKT by genetic mutation.  Malignant pleural mesothelioma (MPM) is a highly aggressive neoplasm associated with asbestos exposure. Previous reports showed that the PI3K/AKT pathway was also frequently activated in MPM, but the molecular mechanisms of its pathway activation in MPM cells remain unclear. In the present study, to determine the frequency and key genetic mutations for the PI3K/AKT activation in MPM, we performed expression and/or mutation analyses of the molecules associated with this pathway including PIK3CA, PTEN, and LKB1, using MPM cell lines.  Methods:  Twenty MPM cell lines were investigated, 13 cell lines we established from Japanese patients and 7 cell lines from Caucasians. The expressions of AKT and PTEN in MPM cell lines were detected with Western blot analysis. Activated AKT was also analyzed with an anti-phospho-AKT antibody. Mutation analyses of PIK3CA, PTEN, and LKB1 were carried out by genomic PCR and direct sequencing.  Results:  Western blot analysis revealed that AKT was prominently activated in 10 (56%) of 18 cell lines studied compared to a normal mesothelial cell line, MeT-5A, under serum-starved condition. The loss of PTEN expression was observed in one cell line, and the following analysis using PTEN complementary DNA synthesized from this cell line showed the existence of an aberrant splicing transcript which lacked exon 2 of the PTEN gene. We demonstrated that this cell line had a homozygous deletion of approximately 40 kb, which encompassed exon 2 of PTEN, while no other mutation was detected in the remaining 19 cell lines. Moreover, no somatic mutation of PIK3CA and LKB1 was detected in the 20 cell lines.  Conclusion: AKT activation was shown in more than half of the MPM cell lines investigated. Homozygous deletion of PTEN was detected in one cell line with elevated AKT activation. Somatic mutations of PIK3CA, LKB1, and PTEN appear to be infrequent in mesothelioma, suggesting that alterations of other upstream molecules of the PI3K/AKT pathway may be responsible for this signaling pathway activation. Thus, although the activation mechanism of the PI3K/AKT pathway in mesothelioma was not fully elucidated, this pathway may be a potential therapeutic target for mesothelioma due to its frequent activation in MPM cells.
A Ligase-Mediated Gene Detection Technique (1988)

An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
Pharmacokinetic Studies in the Rat on a O-Raffinose Polymerized Human Hemoglobin (1992)

We have studied the plasma half-life (T 1/2), oxygen-binding affinity (P50), organ distribution, and excretion of the individual molecular weight (MW) components of human hemoglobin polymerized with periodate-oxidized, ring-opened raffinose (oR poly-Hb), following transfusion in the rat. The model was an isovolemic 50% exchange transfusion in the conscious, chronically catheterized rat. Total plasma Hb levels yielded a (T 1/2) of 10 to 11 hr for oR poly-Hb. The T 1/2 values of individual MW components of the poly-Hb as determined by size-exclusion HPLC were approximately: 4 hr for the monomeric fraction (Hb)1, 9 hr for the dimer (Hb)2, and 15 hr for the fraction representing trimers to nanomers (Hb)3-9. The P50 values of plasma samples containing oR poly-Hb (collected from 0-24 hr after exchange) remained unchanged at 28 +/- 3 mmHg. oR stabilized and polymerized Hb were not excreted via the kidneys. Hepatic and renal distribution as well as plasma and renal clearance were determined by liquid scintillation counting using individual tritium [3H] labelled MW components purified from [3H]-oR poly-Hb: (Hb)1/2, (Hb)1, (Hb)2, (Hb)3&4, and (Hb) greater than 9. In kidney, uptake (determined by the relative concentration of radioactivity) decreased with increasing MW of the labelled component. Conversely, in liver, uptake increased with increasing MW. Plasma and renal clearance results were consistent with those obtained by HPLC analysis. Hematocrit levels returned from a 20% post-transfusion level to normal pre-transfusion levels (44%) within 10 days after the exchange.
Distributed architectures and logical-task decomposition in multimedia surveillance systems (2001)

In the past few years, the development of complex surveillance systems has captured the interest of both the research and industrial worlds. Strong and challenging requirements of modern society are involved in this problem, which aims to increase safety and security in several application domains such as transport, tourism, home and bank security, military applications, etc. At the same time, fast improvements in microelectronics, telecommunications, and computer science make it necessary to consider new perspectives in this field. The main objective of this paper is to investigate, discuss, and evaluate the impact of distributed processing and new communication techniques on multimedia surveillance systems, which represent the so-called third-generation surveillance systems (3 GSSs). In particular, aspects related to the distribution of intelligence among multiple-processing and wide-bandwidth resources are discussed in detail. It is shown how distribution of intelligence can be obtained by a hierarchical architecture that partitions, in a dynamic way, the main logical processing tasks (i.e., representation, recognition, and communication) performed in a 3 GSS physical architecture made up of intelligent cameras, hubs, and central control rooms. The advantages of this solution are pointed out in terms of 1) increased flexibility and reconfigurability and 2) optimal allocation of available processing and bandwidth resources. Finally, a case study is analyzed that allows one to gain a deeper insight into a distributed surveillance system.
Prospective screening of 205 patients with ITP, including diagnosis, serological markers, and the relationship between platelet counts, endogenous thrombopoietin, and circulating antithrombopoietin antibodies (2004)

Immune thrombocytopenia purpura (ITP) is characterized by destruction of circulating platelets and the presence of antiplatelet antibodies. Many of the current immunomodulatory therapies act by reducing platelet destruction and usually do not have a lasting effect. This prospective, exploratory study characterized patients with ITP by identifying their demographic and comorbid clinical factors, use of treatments, serologic markers of autoimmunity, and possible relationships between platelet counts, concentrations of endogenous thrombopoietin (eTPO), and the presence of circulating anti-TPO antibodies. Data including medical history and laboratory evaluations were collected at a single patient visit on 205 patients (19 children, 186 adults). Reported histories revealed a 5% rate of thrombotic/ischemic events. Autoimmune markers including direct antiglobulin test and antinuclear antibodies were found more frequently than in the normal population; antiplatelet antibody testing was not done. eTPO concentrations were comparable to concentrations found in healthy volunteers. Our study confirmed that no significant inverse correlation occurred between circulating concentrations of eTPO and platelet counts in patients with ITP (Spearman r = -0.15). Two of the 205 patients tested (1%) had neutralizing activity of recombinant human TPO in a biological assay; however, this activity was confirmed to be anti-TPO antibody in only 1 patient.