gebhart/patrepeval huggingface dataset:
Characterization of the Role of AMP-Activated Protein Kinase in the Regulation of Glucose-Activated Gene Expression Using Constitutively Active and Dominant Negative Forms of the Kinase (2000)

In the liver, glucose induces the expression of a number of genes involved in glucose and lipid metabolism, e.g., those encoding L-type pyruvate kinase and fatty acid synthase. Recent evidence has indicated a role for the AMP-activated protein kinase (AMPK) in the inhibition of glucose-activated gene expression in hepatocytes. It remains unclear, however, whether AMPK is involved in the glucose induction of these genes. In order to study further the role of AMPK in regulating gene expression, we have generated two mutant forms of AMPK. One of these (alpha1(312)) acts as a constitutively active kinase, while the other (alpha1DN) acts as a dominant negative inhibitor of endogenous AMPK. We have used adenovirus-mediated gene transfer to express these mutants in primary rat hepatocytes in culture in order to determine their effect on AMPK activity and the transcription of glucose-activated genes. Expression of alpha1(312) increased AMPK activity in hepatocytes and blocked completely the induction of a number of glucose-activated genes in response to 25 mM glucose. This effect is similar to that observed following activation of AMPK by 5-amino-imidazolecarboxamide riboside. Expression of alpha1DN markedly inhibited both basal and stimulated activity of endogenous AMPK but had no effect on the transcription of glucose-activated genes. Our results suggest that AMPK is involved in the inhibition of glucose-activated gene expression but not in the induction pathway. This study demonstrates that the two mutants we have described will provide valuable tools for studying the wider physiological role of AMPK.
Transcriptionally active genome regions are preferred targets for retrovirus integration (1990)

We have analyzed the transcriptional activity of cellular target sequences for Moloney murine leukemia virus integration in mouse fibroblasts. At least five of the nine random, unselected integration target sequences studied showed direct evidence for transcriptional activity by hybridization to nuclear run-on transcripts prepared from uninfected cells. At least four of the sequences contained multiple recognition sites for several restriction enzymes that cut preferentially in CpG-rich islands, indicating integration into 5' or 3' ends or flanking regions of genes. Assuming that only a minor fraction (less than 20%) of the genome is transcribed in mammalian cells, we calculated the probability that this association of retroviral integration sites with transcribed sequences is due to chance to be very low (1.6 x 10(-2]. Thus, our results strongly suggest that transcriptionally active genome regions are preferred targets for retrovirus integration.
Cloning and characterization of a fourth human lysyl oxidase isoenzyme (2001)

We report here the complete cDNA sequence and exon-intron organization of the human lysyl oxidase-like (LOXL)3 gene, a new member of the lysyl oxidase (LO) gene family. The predicted polypeptide is 753 amino acids in length, including a signal peptide of 25 residues. The C-terminal region, residues 529-729, contains a LO domain similar to those in the LOX (the first characterized LO isoenzyme), LOXL and LOXL2 polypeptides. It possesses the putative copper binding sequence, and the lysine and tyrosine residues that form the lysyltyrosyl quinone cofactor. The N-terminal region, which is similar to that in LOXL2 but not those in LOX and LOXL, contains four subregions similar to scavenger receptor cysteine-rich domains and a putative nuclear localization signal. Recombinant LOXL3, expressed in HT-1080 cells, was secreted into the culture medium but was not detected by immunofluorescence staining in nuclei. The LOXL3 mRNA is 3.1kb in size and is expressed in many tissues, the highest levels among the tissues studied being seen in the placenta, heart, ovary, testis, small intestine and spleen.
Overview of the FABULOUS EU Project: Final System Performance Assessment With Discrete Components (2015)

In this paper, we present the most comprehensive system demonstrator realized so far inside the FABULOUS project, an EU 7th Framework Program European research project. The architecture proposed in FABULOUS is based on frequency division multiplexed PON and uses a self-coherent and reflective approach in the upstream. The demonstration presented in this paper uses discrete optoelectronics components. We will show system experiments realized with five active optical network units, together with the emulation of other 27 ONUs ASE noise, demonstrating that such network is capable of an upstream transmission of 32 Gb/s over 40 km of dark fiber and an attenuation of 31 dB, in compliancy with ODN class N2 of the latest PON standards. In addition, we will demonstrate, thanks to the flexibility of frequency multiplexing, that the network can adapt its performances to the link conditions, achieving higher aggregate bit rates or higher losses depending on the link quality that every user in the network experiences. To conclude, we will investigate on the feasibility of the required digital signal processing onto FPGA or ASIC platforms.
Sensitive and Rapid Blood and Tissue HPLC Oxime Assay and Pharmacokinetics of MMB-4 in Guinea Pigs and African Green Monkeys (2006)

We developed a rapid and sensitive assay for pyridinium oximes in plasma and tissues. Samples are prepared by acidification and then deproteinized by ultrafiltration. The oximes are measured during sample HPLC fractionation over a cation-exchange column with UV detection. The assay is suitable for analysis of mono- and di-pyridinium oximes, but was originally developed for the measurement of the oxime MMB-4. For both plasma and tissue sources, the LLOD was 0.0005 microgram and the LOQ was 0.001 to 2.5 microgram. The assay requires as little as 50 microliters of whole blood or 30 microliters of tissue homogenate. The assay was used for plasma pharmacokinetic studies from a single intramuscular injection of MMB-4 (dichloride or dimethylsulfonate salt) in nonhuman primate African green monkeys (AGM), and the plasma and tissues of the rodent guinea pig (GP). For the two species, both MMB-4 salts were pharmacokinetically equivalent. In AGM plasma, the Cmax and the area under the curve (AUC) varied dose-dependently with a Tmax of approximately 20 min and mean residence time (MRT) was approximately 92 minutes for all doses of both salts. In GP plasma and muscle tissues, the Cmax and AUC also varied dose- dependently. The plasma Tmax was about 34 to 42 min while the muscle tissue levels peaked at 5-20 min. The tissue concentrations were much lower than the plasma. The tissue levels peaked at 5-20 min depending on the tissue with a concentration ranking of diaphragm &gt; heart &gt; thigh muscle.
Clinical endpoints for drug development in prostate cancer (2008)

The measures of response highlighted in this review, if validated, may improve the current evaluation of novel agents in phase II settings and the potential accelerated approval of these agents.
Identification of regions in interleukin-1 alpha important for activity. (1993)

Saturation mutagenesis of the mature human interleukin-1 alpha (IL-1 alpha) gene has been performed. Following expression in Escherichia coli, the biological and receptor binding activities of the mutant proteins were examined. Most of the molecule could be altered with little effect on either function. More than 3,500 mutants were examined, and only 23 unique amino acid sequences were identified which resulted in an altered ratio of biological to binding activity when compared with wild-type IL-1 alpha. These proteins possessed mutations at 38 of the 159 amino acid residues in IL-1 alpha. Random mutagenesis at several of these positions identified further substitutions that affected activity. Examination of a model for IL-1 alpha localized most of the residues which altered activity along one face of the molecule. This region appears to be distinct from areas of IL-1 which have been postulated to make contact with IL-1 receptor.
Video fields (2016)

Surveillance videos are becoming ubiquitous for monitoring and ensuring security. Nevertheless, mentally fusing the data from multiple video streams covering different regions comes with a high cognitive burden. In this paper we introduce, Video Fields, a novel web-based interactive system to create, calibrate, and render dynamic video-based virtual reality scenes in head-mounted displays, as well as high-resolution wide-field-of-view tiled display walls. Video Fields system automatically projects dynamic videos onto user-defined geometries. It allows users to adjust camera parameters, navigate through time, walk around the scene, and see through the buildings. Our system integrates background modeling and automatic segmentation of moving entities with rendering of video fields. We present two methods to render video fields: early pruning and deferred pruning. Experimental results indicate that the early pruning approach is more efficient than the deferred pruning. Nevertheless, the deferred pruning achieves better results through anti-aliasing and bi-linear interpolation. We envision the use of the system and algorithms introduced in Video Fields for immersive surveillance monitoring in virtual environments.
Absence of Fibroblast Growth Factor 2 Promotes Oligodendroglial Repopulation of Demyelinated White Matter (2002)

This study takes advantage of fibroblast growth factor 2 (FGF2) knock-out mice to determine the contribution of FGF2 to the regeneration of oligodendrocytes in the adult CNS. The role of FGF2 during spontaneous remyelination was examined using two complementary mouse models of experimental demyelination. The murine hepatitis virus strain A59 (MHV-A59) model produces focal areas of spinal cord demyelination with inflammation. The cuprizone neurotoxicant model causes extensive corpus callosum demyelination without a lymphocytic cell response. In both models, FGF2 expression is upregulated in areas of demyelination in wild-type mice. Surprisingly, in both models, oligodendrocyte repopulation of demyelinated white matter was significantly increased in FGF2 -/- mice compared with wild-type mice and even surpassed the oligodendrocyte density of nonlesioned mice. This dramatic result indicated that the absence of FGF2 promoted oligodendrocyte regeneration, possibly by enhancing oligodendrocyte progenitor proliferation and/or differentiation. FGF2 -/- and +/+ mice had similar oligodendrocyte progenitor densities in normal adult CNS, as well as similar progenitor proliferation and accumulation during demyelination. To directly analyze progenitor differentiation, glial cultures from spinal cords of wild-type mice undergoing remyelination after MHV-A59 demyelination were treated for 3 d with either exogenous FGF2 or an FGF2 neutralizing antibody. Elevating FGF2 favored progenitor proliferation, whereas attenuating endogenous FGF2 activity promoted the differentiation of progenitors into oligodendrocytes. These in vitro results are consistent with enhanced progenitor differentiation in FGF2 -/- mice. These studies demonstrate that the FGF2 genotype regulates oligodendrocyte regeneration and that FGF2 appears to inhibit oligodendrocyte lineage differentiation during remyelination.
《劳动——命运和自由》简评 (1985)

本书是作者V.鲁斯和V.阿尔仁舍克10年中进行大量调查研究、理论探讨、讲课和会议发言的结晶。所有这些活动旨在研究人的劳动这个中心问题。他们集中研究了劳动的两个方面:(1)异化方面,指出“围绕着劳动的异化,各种不可知论的思想交织在一起,各种马克思主义学派和非马克思主义学派相互交锋”;(2)劳动分工,认为“劳动分工,一方面与异化密切相关……另一方面又是理解劳动组织和社会结构的出发点。”
Matrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresis (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMatrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresisKerstin. Strupat, Michael. Karas, Franz. Hillenkamp, Christoph. Eckerskorn, and Friedrich. LottspeichCite this: Anal. Chem. 1994, 66, 4, 464–470Publication Date (Print):February 15, 1994Publication History Published online1 May 2002Published inissue 15 February 1994https://pubs.acs.org/doi/10.1021/ac00076a008https://doi.org/10.1021/ac00076a008research-articleACS PublicationsRequest reuse permissionsArticle Views550Altmetric-Citations196LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fluid interaction techniques for the control and annotation of digital video (2003)

We explore a variety of interaction and visualization techniques for fluid navigation, segmentation, linking, and annotation of digital videos. These techniques are developed within a concept prototype called LEAN that is designed for use with pressure-sensitive digitizer tablets. These techniques include a transient position+velocity widget that allows users not only to move around a point of interest on a video, but also to rewind or fast forward at a controlled variable speed. We also present a new variation of fish-eye views called twist-lens, and incorporate this into a position control slider designed for the effective navigation and viewing of large sequences of video frames. We also explore a new style of widgets that exploit the use of the pen's pressure-sensing capability, increasing the input vocabulary available to the user. Finally, we elaborate on how annotations referring to objects that are temporal in nature, such as video, may be thought of as links, and fluidly constructed, visualized and navigated.
Production of β-defensins by human airway epithelia (1998)

Human beta-defensins (HBDs) are antimicrobial peptides that may play a role in mucosal defense. Diminished activity of these peptides has been implicated in the pathogenesis of cystic fibrosis (CF) lung disease. We show that HBD-1 and HBD-2 mRNAs are expressed in excised surface and submucosal gland epithelia from non-CF and CF patients. The pro-inflammatory cytokine interleukin-1beta stimulated the expression of HBD-2 but not HBD-1 mRNA and peptide in primary cultures of airway epithelia. HBD-1 was found in bronchoalveolar lavage (BAL) fluid from normal volunteers, CF patients, and patients with inflammatory lung diseases, whereas HBD-2 was detected in BAL fluid from patients with CF or inflammatory lung diseases, but not in normal volunteers. Both HBD-1 and HBD-2 were found in BAL fluid in concentrations of several ng/ml, and both recombinant peptides showed salt-sensitive bactericidal activity. These data suggest that in the lung HBD-2 expression is induced by inflammation, whereas HBD-1 may serve as a defense in the absence of inflammation.
UV written 13 dB reflection filters in hydrogenated low loss planar silica waveguides (1993)

UV written holographic gratings in hydrogenated GeO2–SiO2 planar waveguides which have given a reflectivity of 13 dB at 1555 nm with a bandwidth of 0.6 nm are reported. The index modulation within the gratings was calculated to be 5 × 10−4. In addition the waveguides are low loss from 1300 to 1600 nm.
Synthesis of deuterium labeled standards of 5‐methoxy‐N,N‐dimethyltryptamine (5‐Meo‐DMT) (2006)

Abstract The Batcho‐Leimgruber strategy was employed to synthesize 5‐[ 2 H 3 ]‐methoxy‐1 H ‐indole 4 from commercially available 5‐hydroxy‐2‐nitrotoluene 1 and CD 3 I. Compound 4 was treated with oxalyl chloride, dimethylamine and lithium aluminum hydride to yield 5‐[ 2 H 3 ]‐methoxy‐ N , N ‐dimethyltryptamine 6 . Copyright © 2006 John Wiley &amp; Sons, Ltd.
Comparison of Drug Concentrations in Blood and Oral Fluid Collected with the Intercept(R) Sampling Device (2010)

The aim of the study was to determine drug concentration ratios between oral fluid collected with the Intercept device and whole blood. Samples of blood and oral fluid were obtained from patients admitted to acute psychiatric treatment and drivers suspected of drugged driving. Samples were analyzed for illegal drugs, benzodiazepines, opioids, carisoprodol, and meprobamate. Drugs were detected in samples of both blood and oral fluid from 59 subjects; altogether, 17 different drugs were found. Concentration ratios between oral fluid and blood were determined for all cases. The distributions of drug concentration ratios were wide for most drugs and do not allow reliable estimations of drug concentrations in blood using concentrations in oral fluid. The median oral fluid/blood drug concentration ratios for the most prevalent drugs were 0.036 diazepam, 0.027 nordiazepam, 7.1 amphetamine, 2.9 methamphetamine, 5.4 codeine, 1.9 morphine, and 4.7 tetrahydrocannabinol. The correlation coefficients between drug concentrations in oral fluid and blood ranged from 0.15 to 0.96 for the six most prevalent drugs.
CD44 binds a chondroitin sulfate proteoglycan, aggrecan (2001)

Here we report that CD44 binds a chondroitin sulfate (CS) proteoglycan, aggrecan, a major component of cartilage. Soluble CD44-IgG and CD44(+) cells bound to aggrecan from rat chondrosarcoma and bovine cartilage, immobilized on microtiter plates. In both cases, binding was blocked by a neutralizing anti-CD44 mAb or by the pretreatment of aggrecan with chondroitinase, but not hyaluronidase or keratanase, indicating that CD44 binds aggrecan in a manner dependent on CS side chains of aggrecan and that hyaluronic acid is not involved in the binding. Structural analysis showed that glycosaminoglycans of aggrecan from rat chondrosarcoma and bovine articular cartilage consist of mainly CS A and a mixture of CS A and C respectively. When immobilized on microtiter plates, both CS A and C bound CD44-IgG, and the reaction was specifically inhibited by an anti-CD44 mAb. In addition, aggrecan augmented apoptosis in cells expressing CD44-Fas chimeric molecules in synergy with a non-blocking anti-CD44 mAb IRAWB14.4, suggesting that CD44-aggrecan interaction can induce oligomerization of the chimeric molecules. These results suggest that aggrecan interacts with CD44 to mediate cell adhesion and to trigger oligomerization of CD44 molecules, which may lead to intracellular signaling.
Rac Regulates Vascular Endothelial Growth Factor Stimulated Motility (2001)

During angiogenesis endothelial cells migrate towards a chemotactic stimulus. Understanding the mechanism of endothelial cell migration is critical to the therapeutic manipulation of angiogenesis and ultimately cancer prevention. Vascular endothelial growth factor (VEGF) is a potent chemotactic stimulus of endothelial cells during angiogenesis. The endothelial cell signal transduction pathway of VEGF represents a potential target for cancer therapy, but the mechanisms of post-receptor signal transduction including the roles of rho family GTPases in regulating the cytoskeletal effects of VEGF in endothelial cells are not understood. Here we analyze the mechanisms of cell migration in the mouse brain endothelial cell line (bEND3). Stable transfectants containing a tetracycline repressible expression vector were used to induce expression of Rac mutants. Endothelial cell haptotaxis was stimulated by constitutively active V12Rac on collagen and vitronectin coated supports, and chemotaxis was further stimulated by VEGF. Osteopontin coated supports were the most stimulatory to bEND3 haptotaxis, but VEGF was not effective in further increasing migration on osteopontin coated supports. Haptotaxis on support coated with collagen, vitronectin, and to a lesser degree osteopontin was inhibited by N17 Rac. N17 Rac expression blocked stimulation of endothelial cell chemotaxis by VEGF. As part of the chemotactic stimulation, VEGF caused a loss of actin organization at areas of cell-cell contact and increased stress fiber expression in endothelial cells which were directed towards pores in the transwell membrane. N17 Rac prevented the stimulation of cell-cell contact disruption and the stress fiber stimulation by VEGF. These data demonstrate two pathways of regulating endothelial cell motility, one in which Rac is activated by matrix/integrin stimulation and is a crucial modulator of endothelial cell haptotaxis. The other pathway, in the presence of osteopontin, is Rac independent. VEGF stimulated chemotaxis, is critically dependent on Rac activation. Osteopontin was a potent matrix activator of motility, and perhaps one explanation for the absence of a VEGF plus osteopontin effect is that osteopontin stimulated motility was inhibitory to the Rac pathway.
Radical Ring-Opening Polymerization of Novel Vinylcyclopropanes Designed as Low Shrinkage Monomers. Structure of the Polymer, Mechanism of the Polymerization, and Volume Change on the Polymerization (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRadical Ring-Opening Polymerization of Novel Vinylcyclopropanes Designed as Low Shrinkage Monomers. Structure of the Polymer, Mechanism of the Polymerization, and Volume Change on the PolymerizationFumio Sanda, Toshikazu Takata, and Takeshi EndoCite this: Macromolecules 1995, 28, 5, 1346–1355Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://pubs.acs.org/doi/10.1021/ma00109a003https://doi.org/10.1021/ma00109a003research-articleACS PublicationsRequest reuse permissionsArticle Views256Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts