gebhart/patrepeval huggingface dataset:
The Biochemical Mechanisms of Resistance by Streptococci to the Antibiotics D-Cycloserine and O-Carbamyl-D-serine* (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Biochemical Mechanisms of Resistance by Streptococci to the Antibiotics D-Cycloserine and O-Carbamyl-D-serine*Richard H. Reitz, Hutton D. Slade, and Francis C. NeuhausCite this: Biochemistry 1967, 6, 8, 2561–2570Publication Date (Print):August 1, 1967Publication History Published online1 May 2002Published inissue 1 August 1967https://pubs.acs.org/doi/10.1021/bi00860a038https://doi.org/10.1021/bi00860a038research-articleACS PublicationsRequest reuse permissionsArticle Views144Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Influence of C1-inhibitor on inflammation, edema and shock. (1993)

C1-Inhibitor (Berinert, C1 INH), a 104 kDa protein, inhibits complement components (C1 esterase) as well as enzymes of the contact phase of coagulation (Factor XII, Factor XI) and kallikrein, thus regulating kinin generation. C1 INH is used for the treatment of the hereditary angioneurotic edema. This paper will give a survey about the evidence in recent literature concerning the potential efficacy of the compound on other diseases associated with shock, capillary leakage and inflammation as well. In our own experiments we evaluated whether the compound could influence acute inflammatory reactions or the severe systemic inflammatory response syndrome (SIRS) as a consequence of an experimental septic shock. To prevent the sepsis-induced DIC we co-infused the thrombin inhibitors AT III or rec. hirudin in combination with C1 INH. Coinfusion of C1-inhibitor (50-200 U/kg x h) with either rec. hirudin or AT III significantly improved survival rate compared to thrombin inhibitor alone.
Smallpox subunit vaccine produced in planta confers protection in mice (2007)

We report here the in planta production of the recombinant vaccinia virus B5 antigenic domain (pB5), an attractive component of a subunit vaccine against smallpox. The antigenic domain was expressed by using efficient transient and constitutive plant expression systems and tested by various immunization routes in two animal models. Whereas oral administration in mice or the minipig with collard-derived insoluble pB5 did not generate an anti-B5 immune response, intranasal administration of soluble pB5 led to a rise of B5-specific immunoglobulins, and parenteral immunization led to a strong anti-B5 immune response in both mice and the minipig. Mice immunized i.m. with pB5 generated an antibody response that reduced virus spread in vitro and conferred protection from challenge with a lethal dose of vaccinia virus. These results indicate the feasibility of producing safe and inexpensive subunit vaccines by using plant production systems.
Continuous ambulatory monitoring of absolute right ventricular pressure and mixed venous oxygen saturation in patients with heart failure using an implantable haemodynamic monitor: results of a 1 year multicentre feasibility study (2001)

This multicentre feasibility study demonstrated the accuracy and stability of sensors implanted in the right ventricle. The IHM-1, using right ventricular pressures and SvO(2), with improved performance, might be useful in the study of pathophysiological mechanisms and treatment interventions in heart failure.
Enteral nutrients potentiate the intestinotrophic action of glucagon-like peptide-2 in association with increased insulin-like growth factor-I responses in rats (2008)

Glucagon-like peptide-2 (GLP-2) is a nutrient-dependent, intestinotrophic hormone derived from posttranslational processing of proglucagon in the distal bowel. GLP-2 is thought to act through indirect mediators, such as IGF-I. We investigated whether intestinal expression of GLP-2 and IGF-I system components are increased with the mucosal growth induced by enteral nutrient (EN) and/or a low dose of GLP-2 in parenterally fed rats. Rats were randomized to four treatment groups using a 2 x 2 design and maintained with parenteral nutrition (PN) for 7 days: PN alone, EN, GLP-2, and EN+GLP-2; n = 7-9. The two main treatment effects are +/-GLP-2 (100 microg.kg body wt(-1).day(-1)) and +/-EN (43% of energy needs, days 4-6). Combination treatment with EN+GLP-2 induced synergistic intestinal growth in ileum, resulting in greater mucosal cellularity, sucrase segmental activity, and gain of body weight (ENxGLP-2, P < 0.04). In addition, EN+GLP-2 induced a significant 28% increase in plasma concentration of bioactive GLP-2, a significant 102% increase in ileal proglucagon mRNA with no change in ileal dipeptidyl peptidase-IV (DPP-IV) specific activity, and significantly reduced plasma DPP-IV activity compared with GLP-2. This indicates that EN potentiates the intestinotrophic action of GLP-2. Proliferation of enterocytes due to GLP-2 infusion was associated with greater expression of ileal proglucagon, GLP-2 receptor, IGF-I, IGF binding protein-3 mRNAs, and greater IGF-I peptide concentration in ileum (P < 0.032). Ileal IGF-I mRNA was positively correlated with expression of proglucagon, GLP-2R, and IGFBP-5 mRNAs (R2 = 0.43-0.56, P < 0.0001). Our findings support the hypothesis that IGF-I is one of the downstream mediators of GLP-2 action in a physiological model of intestinal growth.
A Micromachined Dual-Backplate Capacitive Microphone for Aeroacoustic Measurements (2007)

This paper presents the development of a micro-machined dual-backplate capacitive microphone for aeroacoustic measurements. The device theory, fabrication, and characterization are discussed. The microphone is fabricated using the five-layer planarized-polysilicon SUMMiT V process at Sandia National Laboratories. The microphone consists of a 0.46-mm-diameter 2.25-mum-thick circular diaphragm and two circular backplates. The diaphragm is separated from each backplate by a 2-mum air gap. Experimental characterization of the microphone shows a sensitivity of 390 muV/Pa. The dynamic range of the microphone interfaced with a charge amplifier extends from the noise floor of 41 dB/ radicHz up to 164 dB and the resonant frequency is 178 kHz.
THE MACINTOSH TIBIAL PLATEAU HEMIPROSTHESIS FOR THE RHEUMATOID KNEE (1972)

1. Thirty-nine patients with rheumatoid arthritis who had had a MacIntosh tibial plateau hemiarthroplasty are reviewed. 2. From the clinical findings it is possible to suggest a basis for the selection of suitable patients for the procedure. 3. Eighty-five per cent of the patients obtained relief of pain with good function of the knee. 4. The complications were infection and instability of the prosthesis.
Cardiac troponin T gene expression in muscle. (1992)

We have been analyzing the regulatory regions of the cardiac troponin T gene promoter as a mean toward understanding the mechanisms that govern the transcription of genes which are cross-expressed in cardiac and skeletal muscles during development. By analyzing the activities of mutant cardiac troponin T gene promoter by transient transfection of primary embryonic muscle cells, we showed that both common and distinct elements are required for activity of the cardiac troponin T promoter in these embryonic muscle cells. In skeletal muscle the minimal promoter sufficient to direct activity of the cardiac troponin T promoter is only 99 nucleotides upstream from the transcription initiation site. Within the distal half of this promoter are two tandem copies of a conserved hexanucleotide sequence (5'-CATTCCT-3') we termed the 'M-CAT motif'. Since mutation of either one of the M-CAT motifs abolishes promoter activity, we concluded that both M-CAT motifs are essential for activity of the promoter. The above minimal promoter is insufficient to confer promoter activity in embryonic cardiocytes. In these cells an additional 48-nucleotide region approximately 100 nucleotides upstream of the minimal promoter is needed for efficient promoter activity. We have named this region the 'cardiac element'. This element contains a conserved sequence motif found in other muscle gene promoter. The cardiac element can also act irrespective of orientation and is relatively independent of position, characteristics that are like transcriptional enhancers. This element alone, however, is insufficient to direct cardiac promoter activity. Activity of the 48-nucleotide cardiac element is dependent on either direct or indirect interaction with the downstream M-CAT motifs because mutation of either M-CAT motif also abolishes promoter activity in cardiac cells. The third regulatory region (nucleotide position -550 to -268) is not essential for promoter activity but can enhance activity of the cTNT promoter or a heterologous promoter in both cardiac and skeletal muscle cells by three to five folds. Within this region are sequences which show similarity to motifs found in other muscle gene enhancers. In vitro DNA-protein binding studies showed that the above three regulatory regions interact with nuclear factors. A direct correlation exists between promoter activity and sequence specific binding of a nuclear factor we termed the 'M-CAT binding factor' to the M-CAT motifs. Similar interaction of nuclear regulatory molecules with sequences within the cardiac element and the upstream enhancer region is likely to be the mechanisms which control the action of these regulatory regions.(ABSTRACT TRUNCATED AT 400 WORDS)
Ozone Generation from Oxygen and Air: Discharge Physics and Reaction Mechanisms (1988)

Abstract Industrial ozone generation uses a special high pressure, low temperature electrical discharge which is referred to as the dielectric barrier discharge or silent discharge. The filamentary structure of this discharge and the properties of individual microdischarges are discussed. The main reaction paths for the excited atomic and molecular species in oxygen and air are identified. Possible approaches to obtain high power densities, high ozone generating efficiencies or high ozone concentrations are discussed.
Safety and Immunogenicity of a Bivalent Cytomegalovirus DNA Vaccine in Healthy Adult Subjects (2008)

The safety and immunogenicity data from this trial support further evaluation of VCL-CB01.
Drug-induced progressive multifocal leukoencephalopathy: a case/noncase study in the French pharmacovigilance database (2016)

Progressive multifocal leukoencephalopathy (PML) is an often fatal demyelinating disease of the central nervous system. As effective treatment is unavailable, identification of all drugs that could be associated with PML is essential. The objective of this study was to investigate the putative association of reports of PML and drugs. We used the case/noncase method in the French PharmacoVigilance database (FPVD). Cases were reports of PML in the FPVD between January 2008 and December 2015. Noncases were all other reports during the same period. To assess the association between PML and drug intake, we calculated an adverse drug report odds ratio (ROR) with its 95% confidence interval. We have studied the delay of onset of PML for each drug concerned. Among the 101 cases of PML, 39 drugs were mentioned as suspect. The main therapeutic classes suspected with significant ROR were antineoplastic agents (n = 85), immunosuppressants (n = 67), and corticosteroids. A latent interval from the time of drug initiation to the development of PML is established: the median time to onset was 365 days (123-1095 days). The onset of PML is highly variable and differs among drug classes [from 1 to 96 months (IQR: 39.0-126)]. An association between PML and some immunosuppressant drugs was found as expected, but also with antineoplastic agents and glucocorticoids. An important delay of PML onset after stopping treatment is suspected and should alert prescribers. Prescribers but also patients should be informed about the potential associations with all these drugs. Monitoring could be necessary for many drugs to early detect PML.
Design and Implementation of a Scalable Parallel Direct Solver for Sparse Symmetric Positive Definite Systems: Preliminary Results (1997)

Solving large sparse systems of linear equations is at the core of many problems in engineering and scientific computing. It has long been a challenge to develop parallel formulations of sparse direct solvers due to several different complex steps involved in the process. In this paper, we describe one of the first efficient, practical, and robust parallel solvers for sparse symmetric positive definite linear systems that we have developed and discuss the algorithmic and implementation issues involved in its development. 1 Introduction Solving large sparse systems of linear equations is at the heart of many engineering and scientific computing applications. There are two methods to solve these systems - direct and iterative. Direct methods are preferred for many applications because of various properties of the method and the nature of the application. A wide class of sparse linear systems arising in practice have a symmetric positive definite (SPD) coefficient matrix. The problem is ...
Knowledge‐based model building of proteins: Concepts and examples (1993)

We describe how to build protein models from structural templates. Methods to identify structural similarities between proteins in cases of significant, moderate to low, or virtually absent sequence similarity are discussed. The detection and evaluation of structural relationships is emphasized as a central aspect of protein modeling, distinct from the more technical aspects of model building. Computational techniques to generate and complement comparative protein models are also reviewed. Two examples, P-selectin and gp39, are presented to illustrate the derivation of protein model structures and their use in experimental studies.
Paclitaxel-coated Gianturco–Roubin® II (GR®II) stents reduce neointimal hyperplasia in a porcine coronary in-stent restenosis model (2001)

This study shows that the paclitaxel-coated stents significantly reduced in-stent restenosis without eliciting inflammation.
A Novel Screening Method for Isolating Exopolysaccharide-Deficient Mutants (1998)

A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria.
A randomized comparison of bypassing agents in hemophilia complicated by an inhibitor: the FEIBA NovoSeven Comparative (FENOC) Study (2006)

The development of inhibitory antibodies to factor VIII is a serious complication of hemophilia. FEIBA (factor VIII inhibitor-bypassing activity), an activated prothrombin complex concentrate (aPCC), and NovoSeven, recombinant factor VIIa (rFVIIa), are used as hemostatic bypassing agents in treating patients with inhibitors. The FENOC study was designed to test equivalence of the products in the treatment of ankle, knee, and elbow joint bleeding. A prospective, open-label, randomized, crossover, equivalency design was used. The parameters of interest were the percentage of patients who reported efficacy in response to FEIBA and the percentage that reported efficacy in response to NovoSeven. A difference in these percentages of no more than 15% was determined to be a clinically acceptable magnitude for equivalence of the 2 products. The primary outcome was evaluation 6 hours after treatment. Data for 96 bleeding episodes contributed by 48 participants were analyzed. The criterion for declaring the 2 products equivalent at 6 hours was not met; however, the confidence interval of the difference in percentages of efficacy reported for each product only slightly exceeded the 15% boundary (-11.4%-15.7%), P=.059. FEIBA and NovoSeven appear to exhibit a similar effect on joint bleeds, although the efficacy between products is rated differently by a substantial proportion of patients. This trial was registered at www.clinicaltrials.gov as #NCT00166309.
Redirection of Genetically Engineered CAR-T Cells Using Bifunctional Small Molecules (2015)

Chimeric antigen receptor (CAR)-engineered T cells (CAR-Ts) provide a potent antitumor response and have become a promising treatment option for cancer. However, despite their efficacy, CAR-T cells are associated with significant safety challenges related to the inability to control their activation and expansion and terminate their response. Herein, we demonstrate that a bifunctional small molecule "switch" consisting of folate conjugated to fluorescein isothiocyanate (folate-FITC) can redirect and regulate FITC-specific CAR-T cell activity toward folate receptor (FR)-overexpressing tumor cells. This system was shown to be highly cytotoxic to FR-positive cells with no activity against FR-negative cells, demonstrating the specificity of redirection by folate-FITC. Anti-FITC-CAR-T cell activation and proliferation was strictly dependent on the presence of both folate-FITC and FR-positive cells and was dose titratable with folate-FITC switch. This novel treatment paradigm may ultimately lead to increased safety for CAR-T cell immunotherapy.
A New Route to Organoboron Polymers via Highly Selective Polymer Modification Reactions (2004)

We have developed a highly efficient new method for the introduction of Lewis acidic boron centers into the side chains of organic polymers. Our methodology involves three steps: (i) the controlled polymerization of the functional monomer 4-trimethylsilylstyrene (S−Si), (ii) the exchange of the silyl groups in poly(4-trimethylsilylstyrene) (PS−Si) with BBr3 to give the reactive polymer poly(4-dibromoborylstyrene) (PS−BBr), and (iii) the fine-tuning of the Lewis acidity of the individual boron centers through substituent exchange reactions with nucleophiles. Treatment of PS−BBr with ethoxytrimethylsilane and THF respectively yields the moderately Lewis acidic poly(arylboronate)s PS−BOR (R = Et, 4-bromobutyl). The alkoxy groups in PS−BOR have been exchanged with pinacol to form the air-stable polymer PS−BPin (Pin = pinacolato). Treatment of PS−BBr with 2-thienyltrimethyltin and pentafluorophenylcopper respectively gives the well-defined highly Lewis acidic triarylborane polymers PS−BTh and PS−BPf (Th = 2-thienyl, Pf = 2,3,4,5,6-pentafluorophenyl), which contain triarylborane moieties at every repeat unit along the polymer chain. All polymers have been studied by multinuclear NMR spectroscopy and differential scanning calorimetry. The molecular weights of the arylboronate polymers PS−BOR have been determined by gel permeation chromatography, and the highly selective formation of polymer PS−BPin has been confirmed by static light scattering.
Toward a “Structural BLAST”: Using structural relationships to infer function (2013)

We outline a set of strategies to infer protein function from structure. The overall approach depends on extensive use of homology modeling, the exploitation of a wide range of global and local geometric relationships between protein structures and the use of machine learning techniques. The combination of modeling with broad searches of protein structure space defines a "structural BLAST" approach to infer function with high genomic coverage. Applications are described to the prediction of protein-protein and protein-ligand interactions. In the context of protein-protein interactions, our structure-based prediction algorithm, PrePPI, has comparable accuracy to high-throughput experiments. An essential feature of PrePPI involves the use of Bayesian methods to combine structure-derived information with non-structural evidence (e.g. co-expression) to assign a likelihood for each predicted interaction. This, combined with a structural BLAST approach significantly expands the range of applications of protein structure in the annotation of protein function, including systems level biological applications where it has previously played little role.
A human monoclonal antibody that detects HLA‐A1, A23 and A24 antigens (1993)

We report the production and characterization of a human monoclonal IgM (mu, kappa) antibody recognizing the HLA A1, A23 and A24 antigens. B lymphocytes obtained from a multiparous Japanese woman were transformed in vitro by Epstein-Barr virus, screened with an immune adherence assay, and fused with a murine myeloma cell line, P3-X63-Ag8.653. After subcloning by limiting dilution three times, a stable antibody-secreting hybridoma cell line, 4-35-7, was identified. The culture supernant had a titer of 1:32-64 against each of A1-, A23- and A24-positive lymphocyte panels, and showed complete correlation (r = 1.00) with the A1, A23 and A24 antigens on a lymphocyte panel of 287 unrelated, class I HLA-typed donors by the NIH cytotoxicity assay. Monoclonality of the antibody was ensured by Southern blot analysis of the human immunoglobulin heavy chain gene of 4-35-7. In view of the published data on HLA class I nucleotide sequences, the antibody may recognize an antigeneic determinant including two amino acid residues, Asp-166 and Gly-167, in the alpha 2 helix of the class I molecule that are specific for A1, A23 and A24 so far analyzed.
Discovery of Potent, Orally-Active, and Muscle-Selective Androgen Receptor Modulators Based on an N-Aryl-hydroxybicyclohydantoin Scaffold (2006)

A novel, N-aryl-bicyclohydantoin selective androgen receptor modulator scaffold was discovered through structure-guided modifications of androgen receptor antagonists. A prototype compound (7R,7aS)-10b from this series is a potent and highly tissue-selective agonist of the androgen receptor. After oral dosing in a rat atrophied levator ani muscle model, (7R,7aS)-10b demonstrated efficacy at restoring levator ani muscle mass to that of intact controls and exhibited >50-fold selectivity for muscle over prostate.
Acoustic Emission from Plastic Deformation (1975)

I would like to discuss some recent work at the University of Denver concerning acoustic emission generated during plastic deformation. I must emphasize that this is a preliminary progress report and that the investigation is still in its early stages. I would like to acknowledge two people who have been working with me on this investigation, Robert Wittman of the Denver Research Institute and Frank Higgins, a graduate student of mine at the University of Denver.
SpliceGrapher: detecting patterns of alternative splicing from RNA-Seq data in the context of gene models and EST data (2012)

We propose a method for predicting splice graphs that enhances curated gene models using evidence from RNA-Seq and EST alignments. Results obtained using RNA-Seq experiments in Arabidopsis thaliana show that predictions made by our SpliceGrapher method are more consistent with current gene models than predictions made by TAU and Cufflinks. Furthermore, analysis of plant and human data indicates that the machine learning approach used by SpliceGrapher is useful for discriminating between real and spurious splice sites, and can improve the reliability of detection of alternative splicing. SpliceGrapher is available for download at http://SpliceGrapher.sf.net.