gebhart/patrepeval huggingface dataset:
Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer. (2001)

Caveolin-1 is an integral protein of caveolae, known to play important roles in signal transduction and lipid transport. We demonstrate that caveolin-1 expression is significantly increased in primary and metastatic human prostate cancer after androgen ablation therapy. We also show that caveolin-1 is secreted by androgen-insensitive prostate cancer cells, and that this secretion is regulated by steroid hormones. Significantly, caveolin-1 was detected in the MDL(3) fraction of serum specimens from patients with advanced prostate cancer and to a lesser extent in normal subjects. Conditioned media from high passage caveolin-1 secreting, androgen-insensitive, LNCaP cells stimulated increased viability and clonal growth of low passage, caveolin-1-negative, androgen-sensitive, LNCaP cells in vitro, and this effect was blocked by treating the media with caveolin-1 antibody. i.p. injections of caveolin-1 antibody suppressed the orthotopic growth and spontaneous metastasis of highly metastatic, androgen-insensitive caveolin-1-secreting mouse prostate cancer. Overall, our results establish caveolin-1 as an autocrine/paracrine factor that is associated with androgen-insensitive prostate cancer. We demonstrate the potential for caveolin-1 as a therapeutic target for this important malignancy.
Gene Regulatory Proteins and Their Interaction with DNA (1995)

The selective expression of a gene is achieved through the interaction of protein transcription factors with characteristic DNA sequences located in the regulatory region of the gene, which is usually distinct from the coding region. These proteins contain domains that bind specifically to the DNA sites (or response elements). Some general principles in the design of these DNA-binding domains are described, followed by examples of the different structural classes discovered so far and how they recognize their binding sites.
On the interaction of IgG subclasses with the low affinity Fc gamma RIIa (CD32) on human monocytes, neutrophils, and platelets. Analysis of a functional polymorphism to human IgG2. (1992)

An allotypic form of the low affinity IgG Fc receptor Fc gamma RIIa (CD32), termed low responder (LR) because of its weak reactivity with mouse (m) IgG1, interacts efficiently with human (h) IgG2. Fc gamma RIIaLR is the first known human FcR that binds this IgG subclass. In this study, we analyzed the role of Fc gamma RIIa in binding of stable hIgG-subclass dimers, and in induction of T cell mitogenesis using chimeric anti-CD3 mAb. We demonstrate that the functional polymorphism to hIgG2 is expressed on the majority of Fc gamma R-bearing peripheral blood cells: monocytes, neutrophils, and platelets. We were able to assess Fc gamma RII-mediated IgG-binding without interference of other Fc gamma R-classes, by blockade of Fc gamma RI on monocytes, and by using neutrophils of an individual deficient for the Fc gamma RIIIB gene. This study indicates as subclass specificity: hIgG3 >hIgG1,hIgG2 >> hIgG4 for Fc gamma RIIaLR and hIgG3,hIgG1 >> hIgG2 > hIgG4 for Fc gamma RIIaHR. Comparing the serum hIgG levels of individuals homozygous for the two fc gamma RIIa allotypic forms, we observed significantly lower hIgG2 serum levels in individuals expressing the hIgG2-binding LR allotypic form. This observation may implicate that Fc gamma RIIa regulates hIgG subclass production or turnover in man.
The Cooling Performances Evaluation of Nanofluids in a Compact Heat Exchanger (2012)

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;In the present work a three-dimensional analysis is used to study cooling performances of copper- ethylene glycol nanofluid through the flat tubes of an automobile radiator to evaluate their superiority over the base fluid. A numerical simulation is carried out by solving the governing continuity, momentum and energy equations for laminar flow. The investigation covers Reynolds number, inlet temperature and nanoparticle concentration level in the ranges of 10-125, 348-368K and 0-2% respectively. The effects of using the nanofluid on the heat transfer inside the flat tube are investigated. Numerical results have been validated by comparison of simulations with those available in literature.&lt;/div&gt;&lt;/div&gt;
Humanized anti–interleukin-6 receptor antibody treatment of multicentric Castleman disease (2005)

Multicentric Castleman disease (MCD) is an atypical lymphoproliferative disorder characterized by systemic lymphadenopathy and constitutional inflammatory symptoms. Dysregulated overproduction of interleukin-6 is responsible for the clinical abnormalities. This multicenter prospective study was undertaken to evaluate the safety and efficacy of a humanized anti-human interleukin-6 (IL-6) receptor monoclonal antibody (MRA) in patients with MCD. We report here results of the first 60 weeks of the study enrolling 28 patients. The initial dosing period consisted of 8 infusions of 8 mg/kg MRA administered biweekly. Adjustments in the dose and treatment interval were allowed for each patient in an extension phase after 16 weeks. Within 16 weeks, treatment with MRA consistently alleviated lymphadenopathy and all the inflammatory parameters. Hemoglobin, albumin, and total cholesterol levels, high-density lipoprotein cholesterol values, and body mass index all increased significantly. In addition, fatigue diminished. Chronic inflammatory symptoms were successfully managed over 60 weeks. In 8 (28.6%) patients, the MRA dose was decreased or the treatment interval was extended without exacerbation. Eleven (73.3%) of 15 patients who had received oral corticosteroids before study entry were able to do well on a reduced corticosteroid dose. Most adverse events were mild to moderate in severity. MRA was tolerated well and significantly alleviated chronic inflammatory symptoms and wasting in patients with MCD.
A Role for Fucose α(1−2) Galactose Carbohydrates in Neuronal Growth (2005)

We report a fucose alpha(1-2) galactose-mediated pathway for the modulation of neuronal growth and morphology. Our studies provide strong evidence for the presence of Fucalpha(1-2)Gal glycoproteins and lectin receptors in hippocampal neurons. Additionally, we show that manipulation of Fucalpha(1-2)Gal-associated proteins using small-molecule and lectin probes induces dramatic changes in neuronal morphology. These findings may provide a novel pathway to stimulate neuronal growth and regeneration.
Attribute based data sharing with attribute revocation (2010)

Ciphertext-Policy Attribute Based Encryption (CP-ABE) is a promising cryptographic primitive for fine-grained access control of shared data. In CP-ABE, each user is associated with a set of attributes and data are encrypted with access structures on attributes. A user is able to decrypt a ciphertext if and only if his attributes satisfy the ciphertext access structure. Beside this basic property, practical applications usually have other requirements. In this paper we focus on an important issue of attribute revocation which is cumbersome for CP-ABE schemes. In particular, we resolve this challenging issue by considering more practical scenarios in which semi-trustable on-line proxy servers are available. As compared to existing schemes, our proposed solution enables the authority to revoke user attributes with minimal effort. We achieve this by uniquely integrating the technique of proxy re-encryption with CP-ABE, and enable the authority to delegate most of laborious tasks to proxy servers. Formal analysis shows that our proposed scheme is provably secure against chosen ciphertext attacks. In addition, we show that our technique can also be applicable to the Key-Policy Attribute Based Encryption (KP-ABE) counterpart.
The effect of betamethasone gel in reducing sore throat, cough, and hoarseness after laryngo-tracheal intubation. (2007)

Betamethasone gel, when was used for lubrication of endotracheal tubes pre-operatively, was shown to be effective in decreasing postoperative sore throat, hoarseness, and cough.
Protein and Glycan Mimicry in HIV Vaccine Design (2019)

Antigenic mimicry is a fundamental tenet of structure-based vaccinology. Vaccine strategies for the human immunodeficiency virus type 1 (HIV-1) focus on the mimicry of its envelope spike (Env) due to its exposed location on the viral membrane and role in mediating infection. However, the virus has evolved to minimize the immunogenicity of conserved epitopes on the envelope spike. This principle is starkly illustrated by the presence of an extensive array of host-derived glycans, which act to shield the underlying protein from antibody recognition. Despite these hurdles, a subset of HIV-infected individuals eventually develop broadly neutralizing antibodies that recognize these virally presented glycans. Effective HIV-1 immunogens are therefore likely to involve some degree of mimicry of both the protein and glycan components of Env. As such, considerable efforts have been made to characterize the structure of the envelope spike and its glycan shield. This review summarizes the recent progress made in this field, with an emphasis on our growing understanding of the factors shaping the glycan shield of Env derived from both virus and soluble immunogens. We argue that recombinant mimics of the envelope spike are currently capable of capturing many features of the native viral glycan shield. Finally, we explore strategies through which the immunogenicity of Env glycans may be enhanced in the development of future immunogens.
Surface display of a functional single-chain Fv antibody on staphylococci (1996)

Two different host-vector expression systems designed for cell surface display of chimeric receptors on Staphylococcus xylosus and Staphylococcus carnosus have been evaluated for surface display of a mouse immunoglobulin G1(kappa) [IgG1(kappa)] anti-human IgE single-chain Fv (scFv) antibody fragment. To achieve surface anchoring of the chimeric receptors containing the scFv, the cell surface attachment regions from Staphylococcus aureus protein A were used in both expression systems. The different chimeric receptors could be recovered from cell wall extracts of both S. xylosus and S. carnosus, and surface localization was demonstrated by taking advantage of a serum albumin-binding reporter region present within the two types of receptors. In addition, the two different recombinant staphylococci carrying hybrid receptors containing the scFv were demonstrated to react with the antigen, which was human IgE, in whole-cell enzyme-linked immunosorbent assays. This is the first report of an antibody fragment expressed in a functional form anchored to the surface of gram-positive bacteria. The potential use of recombinant gram-positive bacteria as whole-cell diagnostic devices or alternatives to filamentous phages for surface display of scFv libraries is discussed.
Fully automatic lexicon expansion for domain-oriented sentiment analysis (2006)

This paper proposes an unsupervised lexicon building method for the detection of polar clauses, which convey positive or negative aspects in a specific domain. The lexical entries to be acquired are called polar atoms, the minimum human-understandable syntactic structures that specify the polarity of clauses. As a clue to obtain candidate polar atoms, we use context coherency, the tendency for same polarities to appear successively in contexts. Using the overall density and precision of coherency in the corpus, the statistical estimation picks up appropriate polar atoms among candidates, without any manual tuning of the threshold values. The experimental results show that the precision of polarity assignment with the automatically acquired lexicon was 94% on average, and our method is robust for corpora in diverse domains and for the size of the initial lexicon.
Adaptive quantization scheme for very low bit rate video coding (1999)

This paper presents an efficient two-layer adaptive quantization scheme, which uses Q-N (Quantization step-Number of output bits) mapping tables and fuzzy perceptual classifiers. With this adaptive quantization scheme, the consistent coding quality and good visual effect can be kept under constant bit rate.
Aminoselenenylation of alkenes: syntheses of β-phenylseleno carbamates and β-phenylseleno cyanamides (1990)

β-(Phenylseleno)alkylcarbamates and β-(phenylseleno)alkylcyanamides have been synthesized in good yields by reaction of alkenes with carbamates and cyanamide, respectively, in presence of benzeneselenenyl chloride–silver tetrafluoroborate or N-phenylselenophthalimide–H+. The subsequent reductive or oxidative removal of the phenylseleno group affords alkylcarbamates and alkylcyanamides, and allylic carbamates, cyanamides, and cyanimides.
CA 19-9: handle with care (2013)

Since its inception in the mid-1980s of the 20th century testing for carbohydrate antigen 19-9 (CA 19-9) has raised expectation for an earlier diagnosis and accurate monitoring of several malignant diseases. After almost 30 years, the available evidences have confirmed the appropriateness and usefulness of determining CA 19-9 levels as a prognostic indicator and as a reliable tool for monitoring pancreatic and gastrointestinal cancer, but concerns have been raised about its applications in screening, which is actually not recommended, and in the diagnosis of malignancies, due to several interferences that limit the specificity and to the insufficient sensitivity of this marker. In this paper we aimed to review the basic concepts of CA 19-9 testing and its current applications, with a major focus on the most recent evidences dealing with assay interference, methods comparison and monitoring of malignant diseases. The prognostic value and monitoring recommendations for pancreatic, gastric and colorectal cancers are described in depth.
Computer-based multimedia video conferencing system (1993)

An affordable system level design of a practically prototyped computer-based desktop video conferencing (DVC) system that is capable of delivering full motion analog audio and video is presented. The DVC system is designed to exchange full motion analog video and audio among conference participants using point-to-point and multipoint communication. It is capable of interfacing directly with a codec for video conferencing in the digital domain. The main advantages of this system are low cost, flexibility, and the ability to share applications in an effective manner. The proposed DVC system has been implemented to support 12 users and one additional video/audio input for viewing external television broadcasts.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Three-Dimensional Structure of Full Length Integrin Embedded in Membrane (2011)

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.
Preparation and properties of diamondlike carbon films (1989)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation J. Franks; Preparation and properties of diamondlike carbon films. J. Vac. Sci. Technol. A 1 May 1989; 7 (3): 2307–2310. https://doi.org/10.1116/1.575933 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAVS: Science & Technology of Materials Interfaces and ProcessingJournal of Vacuum Science & Technology A Search Advanced Search |Citation Search
High efficiency retroviral mediated gene transduction into single isolated immature and replatable CD34(3+) hematopoietic stem/progenitor cells from human umbilical cord blood. (1993)

Umbilical cord blood is rich in hematopoietic stem and progenitor cells and has recently been used successfully in the clinic as an alternative source of engrafting and marrow repopulating cells. With the likelihood that cord blood stem/progenitor cells will be used for gene therapy to correct genetic disorders, we evaluated if a TK-neo gene could be directly transduced in a stable manner into single isolated subsets of purified immature hematopoietic cells that demonstrate self-renewed ability as estimated by colony replating capacity. Sorted CD34(3+) cells from cord blood were prestimulated with erythropoietin (Epo), steel factor (SLF), interleukin (IL)-3, and granulocyte-macrophage colony stimulating factor (GM-CSF) and transduced with the gene in two ways. CD34(3+) cells were incubated with retroviral-containing supernatant from TK-neo vector-producing cells, washed, and plated directly or resorted as CD34(3+) cells into single wells containing a single cell or 10 cells. Alternatively, CD34(3+) cells were sorted as a single cell/well and then incubated with viral supernatant. These cells were cultured with Epo, SLF, IL-3, and GM-CSF +/- G418. The TK-neo gene was introduced at very high efficiency into low numbers of or isolated single purified CD34(3+) immature hematopoietic cells without stromal cells as a source of virus or accessory cells. Proviral integration was detected in primary G418-resistant(R) colonies derived from single immature hematopoietic cells, and in cells from replated colonies derived from G418R-colony forming unit-granulocyte erythroid macrophage megakaryocyte (CFU-GEMM) and -high proliferative potential colony forming cells (HPP-CFC). This demonstrates stable expression of the transduced gene into single purified stem/progenitor cells with replating capacity, results that should be applicable for future clinical studies that may utilize selected subsets of stem/progenitor cells for gene therapy.
Covalent DNA-Streptavidin Conjugates as Building Blocks for Novel Biometallic Nanostructures (1998)

Supramolecular aggregates of DNA, RNA, streptavidin, immunoglobulin, and nanocrystalline metal clusters can be generated by self-assembly on the basis of oligonucleotide hybridization (shown schematically). Following selective immunosorption on surface-immobilized antigen, the biometallic hybrid is detectable by electron microscopy.
Enteropathogenic and enterohemorrhagic E. coli: ecology, pathogenesis, and evolution (2013)

EDITORIAL article Front. Cell. Infect. Microbiol., 30 April 2013Sec. Molecular Bacterial Pathogenesis https://doi.org/10.3389/fcimb.2013.00015
The modulation of inflammatory oedema by calcitonin gene‐related peptide (1993)

1 The effect of calcitonin gene-related peptide (CGRP) when given with or as a pretreatment to oedema inducing agents was investigated in the skin and paws of male Wistar and Sprague Dawley rats. 2 Oedema formation at intradermally-injected sites in the skin was measured by a 125I-labelled human serum albumin accumulation technique and paw oedema was measured by a weight displacement technique. 3 CGRP (30 pmol) when given with, or as a 20 min pretreatment, markedly potentiated oedema formation induced by substance P (100 pmol) in rat skin. In comparison, CGRP had little effect on 5-hydroxytryptamine (5-HT, 0.1–3 nmol)-induced oedema when given as a co-injection but significantly potentiated 5-HT-induced oedema when given as a 20 min pretreatment in the skin. Similar results were obtained in both Wistar and Sprague Dawley rats. 4 Pretreatment with CGRP (30 pmol) had little modulatory effect on oedema induced by substance P (100 pmol) in the presence of the vasodilator prostanoid, prostaglandin E1 (PGE1, 850 pmol) in the skin of Wistar rats. 5 Pretreatment with CGRP (30 pmol) caused a non-significant increase in carrageenin (300 μg)-induced oedema in the hind paw of Wistar rats. Capsaicin (100 nmol) given as a pretreatment had little effect on carrageenin-induced oedema. 6 CGRP (30 pmol), given as a pretreatment, had little modulatory effect on 5-HT (3 nmol)-induced oedema in the paw of Wistar rats but a non-significant decrease in paw oedema was observed in Sprague Dawley rats. 7 The results suggest that CGRP, either given together with or as a pretreatment to mediators of increased microvascular permeability, depending on circumstance, can act in a synergistic manner to increase oedema formation. Little evidence was obtained for an anti-inflammatory effect of CGRP in either the rat skin or paw.
Neuromuscular junction autoimmune disease: muscle specific kinase antibodies and treatments for myasthenia gravis (2005)

MuSK antibodies define a form of myasthenia gravis that can be difficult to diagnose, can be life threatening and may require additional treatments. An improved AChR antibody assay may be helpful in patients without AChR or MuSK antibodies. Clinical trials of drugs in other neuroimmunological diseases may help to guide the treatment of myasthenia gravis.
Combination therapy of cyclosporine with methotrexate and gold in rheumatoid arthritis (2 pilot studies). (1994)

CyA in combination with gold or MTX may, over a 6 month period, increase efficacy in patients with refractory RA without significant increase in toxicity.
Endothelin enhances the contractile responsiveness of adult rat ventricular myocytes to calcium by a pertussis toxin-sensitive pathway. (1990)

It has long been assumed that the primary influences regulating cardiac contractility are the extent of mechanical loading of muscle fibers and the activity of the autonomic nervous system. However, the vasoactive peptide endothelin, initially found in vascular endothelium, is among the most potent positively inotropic agents yet described in mammalian myocardium. In isolated adult rat ventricular cells, endothelin's action was slow in onset but very long lasting with an EC50 of 50 pM that approximates the reported KD of the peptide for its receptor in rat heart. When the calcium activity of the buffer superfusing isolated single fura-2-loaded myocytes paced at 1.5 Hz was varied from 0.1 to 0.9 mM [Ca2+]o, 100 pM endothelin increased contractile amplitude with no significant change in diastolic or systolic [Ca2+]i, thus appearing to sensitize the myofilaments to intracellular calcium. Pertussis toxin, or prior exposure to a beta-adrenergic agonist, reduced or abolished the increase in myocyte contractility induced by endothelin. This novel and potent pharmacologic action of endothelin points to the potential importance of local, paracrine factors, perhaps derived from microvascular endothelium or endocardium, in the control of the contractile function of the heart.
Chitinophaga costaii sp. nov., an endophyte of Pinus pinaster, and emended description of Chitinophaga niabensis (2014)

Bacterial strain A37T2(T) was isolated from the endophytic microbial community of a Pinus pinaster tree trunk and characterized. Strain A37T2(T) was Gram-stain-negative, formed rod-shaped cells, and grew optimally at 26-30 °C and at pH 5.5-7.5. The G+C content of the DNA was 46.6 mol%. The major respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were C16 : 1ω5c and iso-C15 : 0, representing 61.7 % of the total fatty acids. The polar lipids consisted of phosphatidylethanolamine, four unidentified aminophospholipids, one unidentified phospholipid, two unidentified aminolipids and three unidentified lipids. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A37T2(T) belonged to the family Chitinophagaceae, forming a distinct branch with Chitinophaga niabensis JS13-10(T) within the genus Chitinophaga. Strain A37T2(T) shared between 92.7 and 95.1 % 16S rRNA gene sequence similarity with the type strains of species of the genus Chitinophaga. The phylogenetic, phenotypic and chemotaxonomic data presented indicate that strain A37T2(T) represents a novel species of the genus Chitinophaga, for which the name Chitinophaga costaii sp. nov. is proposed. The type strain is A37T2(T) ( = CIP 110584(T) = LMG 27458(T)). An emended description of Chitinophaga niabensis JS13-10(T) is also proposed.