gebhart/patrepeval huggingface dataset:
Colonization of the tonsils of calves with Pasteurella haemolytica (1992)

Tonsils of 10 calves were inoculated with Pasteurella haemolytica (PH) and the degree of colonization was followed by collecting sequential tonsil. wash specimens. Tonsils were colonized for at least 3 weeks after instillation of PH into the tonsillar sinus. Calves with colonized tonsils responded with serum and nasal secretion antibody responses to PH and to leukotoxin. Pasteurella haemolytica was detected in nasal mucus specimens of 2 calves during the week after inoculation of the tonsils, but all other specimens were culture-negative. Infectious bovine rhinotracheitis virus-induced respiratory tract disease 25 days later did not elicit a population increase of PH in the tonsils, and did not elicit shedding of PH in nasal mucus.
Role of Fibroblast Growth Factor Receptor Signaling in Prostate Cancer Cell Survival (2001)

These findings reveal an unexpected dependence of prostate cancer cells on FGF receptor signal transduction to traverse the G(2)/M checkpoint. The mechanism for the G(2) arrest is not clear. Our results raise the possibility that FGF-signaling antagonists might enhance the cell death induced by other prostate cancer therapies.
Antibody affinity may influence antigenic modulation of the common acute lymphoblastic leukemia antigen in vitro. (1982)

We have produced a new monoclonal antibody designated BA-3. An extensive side-by-side serologic comparison of BA-3 with the anti-common acute lymphoblastic leukemia antigen (CALLA) monoclonal antibody J-5 was undertaken. Cells examined included established leukemic cell lines, malignant cells from patients with newly diagnosed leukemia/lymphoma, and normal hematopoietic tissues. In all experiments the cellular distribution of the antigens recognized by BA-3 and J-5 were identical when analyzed by immunofluorescent microscopy and the FACS. Iodination of NALM-6 cells, followed by radioimmunoprecipitation and SDS-PAGE, indicated that BA-3 (like J-5) precipitated a glycoprotein of approximately 100,000 daltons. Competitive binding studies using 125I-labeled BA-3 indicated that BA-3 and J-5 were binding to closely associated (if not identical) epitopes on CALLA. Calculation of the equilibrium constant (K value) for BA-3 and J-5, and the approximate number of CALLA molecules per cell, was graphically determined using Scatchard plots. BA-3 and J-5 were shown to have K values of approximately 2.7 x 10(7) M-1 and 7.2 x 10(7) M-1, respectively, with NALM-6 cells expressing 1 x 10(5) to 2 x 10(5) CALLA molecules per cell. Additional studies with BA-3 failed to demonstrate significant antigenic modulation of CALLA in vitro using fresh leukemic cells and leukemic cell lines. Thus, we suggest that antibody affinity may be a significant factor influencing antigenic modulation of CALLA in vitro.
The inflammatory process in the mechanism of decreased serum uric acid concentrations during acute gouty arthritis. (2002)

Decrease in SUA during acute gouty arthritis is associated with increased urinary excretion of uric acid; an inflammatory process may play a role in the mechanism.
Advances in the therapy of rheumatoid arthritis with biological agents (2000)

Rheumatoid arthritis (RA) is the most common chronic systemic autoimmune inflammatory disease. The physiology of inflammation has been systemically studied and this has provided specific therapeutic targets for its modulation. The classical treatments of the disease, such as myocrisin and sulphasalazine, are not always effective at controlling the disease. This has necessitated the development of novel agents for treating RA, most of which are biological in nature and are targeted at specific sites of the inflammatory cascades. Advances in molecular biology have heralded the advent of bio-drugs (recombinant proteins) and gene therapy in which specific genes are introduced to locally enhance in vivo gene expression or suppress gene(s) of interest with a view to downregulating inflammation. Some bio-drugs, such as antitumour necrosis factor-α (TNF-α) antibodies and interleukin-1 receptor antagonists (IL-1Ra) have been tested, shown to be effective, and are licensed for clinical use. The clinical effects tha...
The interferon-inducible 15-kDa ubiquitin homolog conjugates to intracellular proteins. (1992)

We have previously identified a 15-kDa interferon-induced protein that is recognized by affinity-purified rabbit polyclonal antibodies against ubiquitin (Haas, A. L., Ahrens, P., Bright, P. M., and Ankel, H. (1987) J. Biol. Chem. 262, 11315-11323). This ubiquitin cross-reactive protein (UCRP) possesses significant homology to a tandem diubiquitin sequence. Since the biological effects of ubiquitin arise from its covalent ligation to intracellular target proteins, we hypothesized that the multiple cellular responses to inteferon are mediated in part by an analogous conjugation pathway for UCRP. Rabbit polyclonal antibodies specific for UCRP were prepared against homogeneous recombinant protein. Affinity-purified anti-UCRP antibodies detected the induction of UCRP in interferon-beta-treated A549 cells and recognized a group of high molecular weight UCRP conjugates on immunoblots of sodium dodecyl sulfate-polyacrylamide gel electrophoresis-resolved cell extracts. Both free and conjugated UCRP are constitutively present at low levels in untreated cells, suggesting a role for UCRP ligation in normal cellular regulation, and significantly accumulate following interferon treatment. The temporal induction of free UCRP following interferon treatment preceded a delayed increase in UCRP conjugates. Treatment of A549 cells with type I interferons (alpha and beta) strongly induced the expression of free and conjugated UCRP, whereas the response to type II interferon (gamma) was significantly less. A survey of selected cultured cell lines showed differential induction of free versus conjugated UCRP pools in response to interferon. Interferon-beta treatment of A549, MG63, and U937 cells induced high levels of both free and conjugated UCRP, whereas only free UCRP levels increased in Daudi, Namalwa, and K562 cells. These results confirm that UCRP represents a functional ubiquitin homolog participating in a parallel pathway of post-translational ligation and provides a novel mechanism for the response of susceptible cells to the effects of interferon exposure.
The Scientific Drunk and the Lamppost: Massive Sequencing Efforts in Cancer Discovery and Treatment (2013)

The massive resources devoted to genome sequencing of human tumors have produced important data sets for the cancer biology community. Paradoxically, however, these studies have revealed very little new biology. Despite this, additional resources in the United States are slated to continue such work and to expand similar efforts in genome sequencing to mouse tumors. It may be that scientists are "addicted" to the large amounts of data that can be relatively easily obtained, even though these data seem unlikely, on their own, to unveil new cancer treatment options or result in the ultimate goal of a cancer cure. Rather than using more tumor genetic sequences, a better strategy for identifying new treatment options may be to develop methods for analyzing the signaling networks that underlie cancer development, progression, and therapeutic resistance at both a personal and systems-wide level.
Performance virtualization for large-scale storage systems (2004)

Current data centers require storage capacities of hundreds of terabytes to petabytes. Time-critical applications such as online transactions processing depend on getting adequate performance from the storage subsystem: otherwise, they fail. It is difficult to provide predictable quality of service at this level of complexity, because I/O workloads are extremely variable and device behavior is poorly understood. Ensuring that unrelated but competing workloads do not affect each other's performance is still more difficult, and equally necessary. We present SLEDS (Service Level Enforcement Discipline for Storage), a distributed controller that provides statistical performance guarantees on a storage system built from commodity components. SLEDS can adaptively handle unpredictable workload variations so that each client continues to get the performance it needs even in the presence of misbehaving, competing peers. After evaluating the SLEDS on a heterogeneous mid-range storage system, we found that it is vastly superior to the raw system in its ability to provide performance guarantees, while only introducing a negligible overhead.
Range dependence of the response of a spherical head model (1998)

The head-related transfer function (HRTF) varies with range as well as with azimuth and elevation. To better understand its close-range behavior, a theoretical and experimental investigation of the HRTF for an ideal rigid sphere was performed. An algorithm was developed for computing the variation in sound pressure at the surface of the sphere as a function of direction and range to the sound source. The impulse response was also measured experimentally. The results may be summarized as follows. First, the experimental measurements were in close agreement with the theoretical solution. Second, the variation of low-frequency interaural level difference with range is significant for ranges smaller than about five times the sphere radius. Third, the impulse response reveals the source of the ripples observed in the magnitude response, and provides direct evidence that the interaural time difference is not a strong function of range. Fourth, the time delay is well approximated by well-known ray-tracing formula due to Woodworth and Schlosberg. Finally, except for this time delay, the HRTF for the ideal sphere appears to be minimum-phase, permitting exact recovery of the impulse response from the magnitude response in the frequency domain.
Modulation of natural IgM binding and complement activation by natural IgG antibodies: a role for IgG anti-Gal α1-3Gal antibodies (1996)

The most abundant natural IgG Abs in human serum are thought to be Abs specific for Gal alpha1-3Gal, a carbohydrate expressed in lower mammals. IgG Abs specific for Gal alpha1-3Gal have been postulated to contribute to host defense and to participate in the rejection of interspecies organ grafts. Our previous studies indicated, however, that IgM and not IgG anti-Gal alpha1-3Gal Abs activate complement on foreign surfaces, and thus the physiologic role of IgG anti-Gal alpha1-3Gal remains uncertain. We tested whether the IgG anti-Gal alpha1-3Gal in a human serum might in fact compete with IgM for binding and thus modulate complement fixation by IgM. Several lines of evidence suggested such competition might occur. First, the functional avidity of IgG and IgM for Gal alpha1-3Gal on cell surfaces were nearly within the same order of magnitude, and in some sera the molar concentrations of IgG and IgM anti-Gal alpha1-3Gal were comparable. Second, binding of human IgM to Gal alpha1-3Gal on cell surfaces was inversely correlated with the concentration of IgG anti-Gal alpha1-3Gal in serum. Third, combination of IgG and IgM Abs specific for Gal alpha1-3Gal demonstrated direct competition for binding. The presence of IgG anti-Gal alpha1-3Gal, which was predominantly IgG2, attenuated by up to 80% the fixation of C1q mediated by IgM, presumably by competing for antigenic sites recognized by IgM Abs that fix complement. Thus, IgG Abs specific for Gal alpha1-3Gal modulate complement activation by IgM specific for that structure and might in this way modulate the consequences that ensue when human blood is brought into contact with foreign organisms or xenogenic cells.
Spread source/drain (SSD) MOSFET using selective silicon growth for 64 Mbit DRAMs (2003)

A novel MOSFET structure which has a small occupied area for 64-Mb DRAMs (dynamic RAMs) is proposed. The source-drain regions are raised by using a selective silicon growth technique. Because of lateral growth of the silicon over the gate and the field, the contact area can overlap the gate and the field. Moreover, the shallow source-drain junction of the raised source-drain structure realizes the reduction of the gate length and the isolation spacing. As a result, the MOSFET can minimize the total occupied area. It has been verified that this MOSFET has the potential to realize high-density LSIs such as 64-Mb DRAMs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Dipeptidyl peptidase IV, a kidney brush-border serine peptidase (1976)

Dipeptidyl peptidase IV, an enzyme that releases dipeptides from substrates with N-terminal sequences of the forms X-Pro-Y or X-Ala-Y, was purified 300-fold from pig kidney cortex. The kidney is the main source of the enzyme, where it is one of the major microvillus-membrane proteins. Several other tissues contained demonstrable activity against the usual assay substrate glycylproline 2-naphthylamide. In the small intestine this activity was greatly enriched in the microvillus fraction. In all tissues examined, the activity was extremely sensitive to inhibition by di-isopropyl phosphorofluoridate (Dip-F), but relatively resistant to inhibition by phenylmethylsulphonyl fluoride. It is a serine proteinase which may be covalently labelled with [32P]Dip-F, and is the only enzyme of this class in the microvillus membrane. The apparent subunit mol.wt. estimated by sodium dodecyl-sulphate/polyacrylamide-gel electrophoresis and by titration with [32P]Dip-F was 130 000. Gel-filtration and sedimentation-equilibrium methods gave values in the region of 280 000, which is consistent with a dimeric structure, a conclusion supported by electron micrographs of the purified enzyme. Among other well-characterized serine proteinases, this enzyme is unique in its membrane location and its large subunit size. Investigation of the mode of attack of the peptidase on oligopeptides revealed that it could hydrolyse certain N-blocked peptides, e.g. Z-Gly-Pro-Leu-Gly-Pro. In this respect it is acting as an endopeptidase and as such may merit reclassification and renaming as microvillus-membrane serine peptidase.
Human Chorionic Gonadotropin Expression by Bladder Cancers: Biology and Clinical Potential (1991)

No AccessJournal of UrologyReview Article1 Mar 1991Human Chorionic Gonadotropin Expression by Bladder Cancers: Biology and Clinical Potential R.K. Iles and T. Chard R.K. IlesR.K. Iles and T. ChardT. Chard View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)38367-2AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1991 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 145Issue 3March 1991Page: 453-458 Advertisement Copyright & Permissions© 1991 by The American Urological Association Education and Research, Inc.Keywordsbladder neoplasmsgonadotropins, chorionicbiological markersMetricsAuthor Information R.K. Iles More articles by this author T. Chard More articles by this author Expand All Advertisement PDF downloadLoading ...
Decreasing Phosphorus Solubility in Poultry Litter with Aluminum, Calcium, and Iron Amendments (1994)

Abstract Arkansas produces approximately one billion broilers ( Gallus gallus domesticus ) each year. Phosphorous runoff from fields receiving poultry fitter is believed to be one of the primary factors affecting water quality in northwest Arkansas. Poultry litter contains ≈20 g P kg −1 , of which ≈2 g P kg −1 is water soluble. The objective of this study was to determine if soluble P levels could be reduced in poultry litter with Al, Ca, and/or Fe amendments. Poultry litter was amended with alum, sodium aluminate, quick lime, slaked lime, calcitic limestone, dolomitic limestone, gypsum, ferrous chloride, ferric chloride, ferrous sulfate, and ferric sulfate, and incubated in the dark at 25 °C for 1 wk. The Ca treatments were tested with and without CaF 2 additions in an attempt to precipitate fluorapatite. At the end of the incubation period, the fitter was extracted with deionized water and water soluble P determined. Water soluble P levels in the poultry litter were reduced from &gt;2,000 mg P kg −1 litter to &lt;1 mg P kg −1 litter with the addition of alum, quick lime, slaked lime, ferrous chloride, ferric chloride, ferrous sulfate, and ferric sulfate under favorable pH conditions. Gypsum and sodium aluminate reduced water soluble P levels by 50 to 60%. Calcitic and dolomitic limestone were less effective. The results of this study suggest that treating litter prior to field application with some of these compounds could significantly reduce the amount of soluble P in runoff from litter‐amended pastures. Therefore, chemical additions to reduce soluble P in litter may be a best management practice in situations where eutrophication of adjacent water bodies due to P runoff has been identified. Preliminary calculations indicate that this practice may be economically feasible. More research is needed, however, to determine any beneficial and/or detrimental aspects of this practice.
Analytical Validation and Capabilities of the Epic CTC Platform: Enrichment-Free Circulating Tumour Cell Detection and Characterization (2015)

The Epic Platform was developed for the unbiased detection and molecular characterization of circulating tumour cells (CTCs). Here, we report assay performance data, including accuracy, linearity, specificity and intra/inter-assay precision of CTC enumeration in healthy donor (HD) blood samples spiked with varying concentrations of cancer cell line controls (CLCs). Additionally, we demonstrate clinical feasibility for CTC detection in a small cohort of metastatic castrate-resistant prostate cancer (mCRPC) patients. The Epic Platform demonstrated accuracy, linearity and sensitivity for the enumeration of all CLC concentrations tested. Furthermore, we established the precision between multiple operators and slide staining batches and assay specificity showing zero CTCs detected in 18 healthy donor samples. In a clinical feasibility study, at least one traditional CTC/mL (CK+, CD45-, and intact nuclei) was detected in 89 % of 44 mCRPC samples, whereas 100 % of samples had CTCs enumerated if additional CTC subpopulations (CK-/CD45- and CK+ apoptotic CTCs) were included in the analysis. In addition to presenting Epic Platform's performance with respect to CTC enumeration, we provide examples of its integrated downstream capabilities, including protein biomarker expression and downstream genomic analyses at single cell resolution.
Wet microcontact printing (µCP) for micro-reservoir drug delivery systems (2013)

When micro-reservoir-type drug delivery systems are fabricated, loading solid drugs in drug reservoirs at microscale is often a non-trivial task. This paper presents a simple and effective solution to load a small amount of drug solution at microscale using 'wet' microcontact printing (µCP). In this wet µCP, a liquid solution containing drug molecules (methylene blue and tetracycline HCl) dissolved in a carrier solvent was transferred to a target surface (drug reservoir) by contact printing process. In particular, we have investigated the dependence of the quantity and morphology of transferred drug molecules on the stamp size, concentration, printing times, solvent types and surfactant concentration. It was also found that the repetition of printing using a non-volatile solvent such as polyethylene glycol (PEG) as a drug carrier material actually increased the transferred amount of drug molecules in proportion to the printing times based on asymmetric liquid bridge formation. Utilizing this wet µCP, drug delivery devices containing different quantity of drugs in micro-reservoirs were fabricated and their performance as controlled drug delivery devices was demonstrated.
Antibacterial and antifungal activity of chitosan coated iron oxide nanoparticles (2017)

Chitosan coated Fe<sub>3</sub>O<sub>4</sub> NPs are effective antimicrobial agents and so may be developed as a microbial resistant coating for biomedical devices.
Development, differentiation, and maturation of Kupffer cells (1998)

Primitive macrophages first develop in the murine and human yolk sac and then differentiate into fetal macrophages. Primitive or fetal macrophages enter the blood stream and migrate into the fetal liver. Fetal macrophages possess a high proliferative capacity and express antigens and peroxidase activity of resident macrophages with the progress of gestation; they become mature and then transform into Kupffer cells. In contrast, myelopoiesis and monocytopoiesis are not active in yolk sac hematopoiesis and in the early stages of hepatic hematopoiesis. Precursor cells of primitive or fetal macrophages exist and granulocyte/macrophage colony-forming cells develop in the yolk sac and in the early stages of fetal liver development, whereas macrophage colony-forming cells emerge and increase later in fetal liver development. In vitro, similar colonies were formed from each fetal hematopoietic cell in the presence of different macrophage growth factors. During culturing of the yolk sac cells and hepatic hematopoietic cells on a monolayer of mouse stromal cell line, ST2, primitive or fetal macrophage colonies developed before the formation of monocyte colonies, suggesting the existence of a direct pathway of differentiation from primitive macrophages into fetal macrophages during ontogeny. In severely monocytopenic mice induced by the administration of strontium-89, Kupffer cells have a proliferative capacity and are maintained by self-renewal. In macrophage colony-stimulating factor (M-CSF)-deficient (op/op) mice, the number of Kupffer cells is reduced, and they are characterized by immature morphology and a proliferative potential similar to that of primitive or fetal macrophages during ontogeny. Immediately after the administration of M-CSF to op/op mice, Kupffer cells start proliferating and become mature. This finding indicates that M-CSF plays an important role in the differentiation and proliferation of Kupffer cells.
Internalization and degradation of monoclonal antibody chCE7 by human neuroblastoma cells (1994)

Internalization and cellular degradation of a chimeric monoclonal anti-neuroblastoma antibody (MAb chCE7) is described. Immunofluorescence localization showed temperature-dependent redistribution of MAb chCE7 from the cell surface at 0 degrees C to vesicular structures after heating to 37 degrees C. SKN-AS cells which were pulse-labelled at 0 degrees C with radioiodinated chCE7 released 50% of the initial cell-bound radioactivity into the medium after 20 hr at 37 degrees C. Low-molecular-weight radioactivity in the medium was identified as iodotyrosine and the major intracellular degradation product was found to be an antibody fragment of 43 kDa. Degradation was blocked by chloroquine, an inhibitor of lysosomal function. MAb chCE7 binds to a carbohydrate epitope, as treatment with tunicamycin abolishes cell binding. In adherent cells in culture, inhibition of protein synthesis by cycloheximide affected cell-surface binding sites for chCE7 in a biphasic manner, with an initial increase followed by long-term decrease. Expression of binding sites was also found to be inhibited by sodium azide, by the lysosomotropic drug chloroquine and by Brefeldin A, a drug which prevents export of newly synthetized proteins to the cell surface. When cells were treated with high doses of chCE7 up to 24 hr and cell-surface binding sites were then measured after an acidic buffer wash, no loss of surface binding sites was found, indicating that pretreatment with MAb chCE7 does not induce down-regulation of its binding sites.