gebhart/patrepeval huggingface dataset:
CEACAM6 Is a Novel Biomarker in Pancreatic Adenocarcinoma and PanIN Lesions (2005)

CEACAM6 is a novel biomarker for pancreatic adenocarcinoma. CEACAM6 warrants further evaluation as both a prognostic factor and a therapeutic target in pancreatic cancer.
hTERT is a critical determinant of telomerase activity in renal-cell carcinoma (1998)

Telomerase is a ribonucleoprotein enzyme which stabilizes chromosomal structure, thereby inducing cellular immortality. Three major subunits composing telomerase complex have been cloned, designated hTR (human telomerase RNA), TPI (telomerase-associated protein I), and hTERT (human telomerase reverse transcriptase). In the present study, a total of 36 renal-cell carcinomas (RCC) and adjacent normal tissues, as well as cell lines derived from RCC or normal kidney, were examined for the expression of each telomerase subunit and telomerase activity. RT-PCR analyses revealed that hTR and TP I mRNA were constitutively expressed both in tumor and in normal tissues. In contrast, hTERT mRNA was expressed in most tumors, but not in normal tissues. Telomeric-repeat-amplification-protocol (TRAP) assay revealed that more than 80% of RCC tumor tissue exhibited telomerase activity, while none of the adjacent normal tissue did. There was a significant association of telomerase activity with expression of hTERT mRNA, but not with TPI mRNA or hTR expression. Two cell lines, derived from RCC and cervical cancer, expressed telomerase activity and hTERT mRNA, while normal renal cortical epithelial cells expressed neither of them. These findings suggest that hTERT plays a critical role in determining the enzymatic activity of human telomerase, and that up-regulation of hTERT probably plays a role in the progression of human cancers.
Imaging Electric Properties of Biological Tissues by RF Field Mapping in MRI (2010)

The electric properties (EPs) of biological tissue, i.e., the electric conductivity and permittivity, can provide important information in the diagnosis of various diseases. The EPs also play an important role in specific absorption rate calculation, a major concern in high-field MRI, as well as in nonmedical areas such as wireless telecommunications. The high-field MRI system is accompanied by significant wave propagation effects, and the RF radiation is dependent on the EPs of biological tissue. On the basis of the measurement of the active transverse magnetic component of the applied RF field (known as B(1)-mapping technique), we propose a dual-excitation algorithm, which uses two sets of measured B(1) data to noninvasively reconstruct the EPs of biological tissues. The finite-element method was utilized in 3-D modeling and B(1) field calculation. A series of computer simulations were conducted to evaluate the feasibility and performance of the proposed method on a 3-D head model within a TEM coil and a birdcage coil. Using a TEM coil, when noise free, the reconstructed EP distribution of tissues in the brain has relative errors of 12%-28% and correlated coefficients of greater than 0.91. Compared with other B(1)-mapping-based reconstruction algorithms, our approach provides superior performance without the need for iterative computations. The present simulation results suggest that good reconstruction of EPs from B1 mapping can be achieved.
Specific effect of levetiracetam in experimental human pain models* (2005)

Levetiracetam has an analgesic effect in the electrical sural nerve stimulation pain model, but it did not increase temporal pain summation threshold. Levetiracetam may still be effective in clinical neuropathic pain.
Human Anti-CD38 Autoantibodies Raise Intracellular Calcium and Stimulate Insulin Release in Human Pancreatic Islets (2001)

CD38 is involved in transmembrane signaling in many cell types; anti-CD38 autoantibodies have been described in diabetic patients. We tested whether human anti-CD38 antibodies possess signaling properties by measuring their ability to raise intracellular calcium ([Ca2+]i) using the fluo-3-acetoxymethyl ester method in a human-derived T-cell line (Jurkat T-cells, expressing high levels of surface CD38) and in dispersed human islet cells from normal donors. In Jurkat T-cells, 11 of 19 anti-CD38-positive sera raised [Ca2+]i (by > or =20% of baseline), whereas no [Ca2+]i-mobilizing activity was found in 27 anti-CD38-negative sera (chi2 = 20.5, P < 0.0001). In dispersed human islet cells, 5 of 11 anti-CD38-positive sera (and none of three anti-CD38-negative sera) raised [Ca2+]i significantly. When preincubated with Staphylococcus aureus protein A to remove IgG, anti-CD38-positive sera showed a 70 +/- 5% reduction in [Ca2+]i-mobilizing activity. Preincubation with CD38-transfected NIH-3T3 fibroblasts, but not with mock-transfected NIH-3T3 cells, abolished [Ca2+]i mobilization. In blocking experiments, preincubation with nonagonistic anti-CD38 monoclonal antibodies also prevented [Ca2+]i mobilization. In cultured human islets, anti-CD38-positive sera exhibiting [Ca2+]i-mobilizing activity in Jurkat T-cells (n = 6) significantly stimulated insulin release at 3.3 mmol/l glucose (median [interquartile range] 738 microU/ml [234], P = 0.0001 vs. 320 [52] microU/ml of control), whereas 6 anti-CD38-positive sera without [Ca2+]i-mobilizing activity and 10 anti-CD38-negative did not. In further incubations, the five anti-CD38-positive sera displaying [Ca2+]i-mobilizing activity in dispersed islet cells significantly stimulated insulin release at both 3.3 mmol/l glucose (2.2 +/- 0.3% of insulin islet content, P < 0.002 vs. 1.2 +/- 0.1% of control) and 16.7 mmol/l glucose (3.7 +/- 0.3 vs. 2.3 +/- 0.3%, P < 0.002). We conclude that human anti-CD38 autoantibodies with agonistic properties on the CD38 effector system occur in nature; in human islets, their [Ca2+]i-mobilizing activity is coupled with the ability to stimulate insulin release.
Reinforcement Learning: A Survey (1996)

This paper surveys the field of reinforcement learning from a computer-science perspective. It is written to be accessible to researchers familiar with machine learning. Both the historical basis of the field and a broad selection of current work are summarized. Reinforcement learning is the problem faced by an agent that learns behavior through trial-and-error interactions with a dynamic environment. The work described here has a resemblance to work in psychology, but differs considerably in the details and in the use of the word ``reinforcement.'' The paper discusses central issues of reinforcement learning, including trading off exploration and exploitation, establishing the foundations of the field via Markov decision theory, learning from delayed reinforcement, constructing empirical models to accelerate learning, making use of generalization and hierarchy, and coping with hidden state. It concludes with a survey of some implemented systems and an assessment of the practical utility of current methods for reinforcement learning.
Substitution of asparagine residues in Aspergillus awamori glucoamylase by site-directed mutagenesis to eliminate N-glycosylation and inactivation by deamidation (1994)

Aspergillus awamori glucoamylase is a secreted glycoprotein containing N-linked carbohydrate recognition sites at Asn-171, Asn-182 and Asn-395. Site-directed mutagenesis was performed at Asn-182 and Asn-395 to determine whether these residues were N-glycosylated by Saccharomyces cerevisiae, to investigate the function of any glycans linked to them, and to determine the effect of their deamidation on glucoamylase thermostability. Asn-171 and Asn-395, but not Asn-182, were N-glycosylated. Deletion of the glycan N-linked to Asn-395 did not affect specific activity, but greatly decreased enzyme secretion and thermostability. The mutant lacking the N-glycan linked to Asn-395 was synthesized very slowly, and was more associated with cell membrane components and susceptible to proteinase degradation than were wild-type or other mutant glucoamylases. Its secreted form was 30-fold less thermostable than wild-type enzyme at pH 4.5. Replacement of Asn-182 by Gln to eliminate deamidation at this site did not change glucoamylase specific activity or thermostability, while replacement by Asp decreased specific activity about 25%, but increased thermostability moderately at pH 4.5 below 70 degrees C. Both mutations of Asn-182 increased glucoamylase production.
Episome-generated N-myc antisense RNA restricts the differentiation potential of primitive neuroectodermal cell lines. (1991)

Neuroectodermal tumors of childhood provide a unique opportunity to examine the role of genes potentially regulating neuronal growth and differentiation because many cell lines derived from these tumors are composed of at least two distinct morphologic cell types. These types display variant phenotypic characteristics and spontaneously interconvert, or transdifferentiate, in vitro. The factors that regulate transdifferentiation are unknown. Application of antisense approaches to the transdifferentiation process has allowed us to explore the precise role that N-myc may play in regulating developing systems. We now report construction of an episomally replicating expression vector designed to generate RNA antisense to part of the human N-myc gene. Such a vector is able to specifically inhibit N-myc expression in cell lines carrying both normal and amplified N-myc alleles. Inhibition of N-myc expression blocks transdifferentiation in these lines, with accumulation of cells of an intermediate phenotype. A concomitant decrease in growth rate but not loss of tumorigenicity was observed in the N-myc nonamplified cell line CHP-100. Vector-generated antisense RNA should allow identification of genes specifically regulated by the proto-oncogene N-myc.
Learning to Localize Using a LiDAR Intensity Map (2020)

In this paper we propose a real-time, calibration-agnostic and effective localization system for self-driving cars. Our method learns to embed the online LiDAR sweeps and intensity map into a joint deep embedding space. Localization is then conducted through an efficient convolutional matching between the embeddings. Our full system can operate in real-time at 15Hz while achieving centimeter level accuracy across different LiDAR sensors and environments. Our experiments illustrate the performance of the proposed approach over a large-scale dataset consisting of over 4000km of driving.
LOXL2 in Epithelial Cell Plasticity and Tumor Progression (2012)

Several members of the lysyl oxidase family have recently emerged as important regulators of tumor progression. Among them, LOXL2 has been shown to be involved in tumor progression and metastasis of several tumor types, including breast carcinomas. Secreted LOXL2 participates in the remodeling of the extracellular matrix of the tumor microenvironment, in a similar fashion to prototypical lysyl oxidase. In addition, new intracellular functions of LOXL2 have been described, such as its involvement in the regulation of the epithelial-to-mesenchymal transition, epithelial cell polarity and differentiation mediated by transcriptional repression mechanisms. Importantly, intracellular (perinuclear) expression of LOXL2 is associated with poor prognosis and distant metastasis of specific tumor types, such as larynx squamous cell carcinoma and basal breast carcinomas. These recent findings open new avenues for the therapeutic utility of LOXL2.
Detection of Secondhand Cigarette Smoke via Nicotine Using Conductive Polymer Films (2013)

A sensing element has been developed that can reliably detect secondhand and thirdhand tobacco smoke in real time through the adsorption of ambient nicotine vapor. The device was calibrated to the number of smoked cigarettes and to nicotine concentration. Development of integrated personal sensors to record exposure to SHS using this technology is currently underway.
Biofilms Formed by Nontypeable Haemophilus influenzae In Vivo Contain both Double-Stranded DNA and Type IV Pilin Protein (2007)

Nontypeable Haemophilus influenzae (NTHI) strains are members of the normal human nasopharyngeal flora, as well as frequent opportunistic pathogens of both the upper and lower respiratory tracts. Recently, it has been shown that NTHI can form biofilms both in vitro and in vivo. NTHI strains within in vitro-formed biofilms differentially express both epitopes of lipooligosaccharide (LOS) and the outer membrane proteins P2, P5, and P6, whereas those generated either in a 96-well plate assay in vitro or in a mammalian host have been shown to incorporate a specific glycoform of sialylated LOS within the biofilm matrix. While DNA has been identified as a key component of the biofilm matrix formed in vitro by several bacterial pathogens, here we demonstrate for the first time that in addition to sialylated LOS, the biofilm formed by NTHI in vivo contains both type IV pilin protein and a significant amount of double-stranded DNA. The DNA appeared to be arranged in a dense interlaced meshwork of fine strands as well as in individual thicker "ropes" that span water channels, suggesting that DNA could be imparting structural stability to the biofilm produced by NTHI in vivo. The presence of type IV pilin protein both appearing as small aggregates within the biofilm matrix and tracking along DNA strands supports our observations which showed that type IV pili are expressed by NTHI during experimental otitis media when these bacteria form a biofilm in the middle ear space.
Pathogenesis of persistent lymphatic vessel hyperplasia in chronic airway inflammation (2005)

Edema occurs in asthma and other inflammatory diseases when the rate of plasma leakage from blood vessels exceeds the drainage through lymphatic vessels and other routes. It is unclear to what extent lymphatic vessels grow to compensate for increased leakage during inflammation and what drives the lymphangiogenesis that does occur. We addressed these issues in mouse models of (a) chronic respiratory tract infection with Mycoplasma pulmonis and (b) adenoviral transduction of airway epithelium with VEGF family growth factors. Blood vessel remodeling and lymphangiogenesis were both robust in infected airways. Inhibition of VEGFR-3 signaling completely prevented the growth of lymphatic vessels but not blood vessels. Lack of lymphatic growth exaggerated mucosal edema and reduced the hypertrophy of draining lymph nodes. Airway dendritic cells, macrophages, neutrophils, and epithelial cells expressed the VEGFR-3 ligands VEGF-C or VEGF-D. Adenoviral delivery of either VEGF-C or VEGF-D evoked lymphangiogenesis without angiogenesis, whereas adenoviral VEGF had the opposite effect. After antibiotic treatment of the infection, inflammation and remodeling of blood vessels quickly subsided, but lymphatic vessels persisted. Together, these findings suggest that when lymphangiogenesis is impaired, airway inflammation may lead to bronchial lymphedema and exaggerated airflow obstruction. Correction of defective lymphangiogenesis may benefit the treatment of asthma and other inflammatory airway diseases.
Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II. (1981)

A low molecular weight polypeptide with multiplication-stimulating activity (MSA) was isolated from serum-free media conditioned by a clone of normal Buffalo rat liver cells. The purification of MSA was achieved by gel permeation chromatography of the acid-soluble growth-promoting activity on Bio-Gel P-10 in 1 M acetic acid and followed by reversed phase high pressure liquid chromatography on muBondapak C18 support using a linear gradient of acetonitrile in 0.05% trifluoroacetic acid. The primary structure of MSA has been determined. MSA is a single chain polypeptide of 67 residues, with a calculated molecular weight of 7,484, and displays 93% homology with the functionally related human insulin-like growth factor II (IGF-II). A comparison between the sequences of rat MSA and human IGF-II reveals only five amino acid substitutions. Based on the extensive amino acid sequence homology, we propose the term rat IGF-II for this newly isolated polypeptide.
Silicon Photonics in High Performance Computing (2010)

Recent results in the application of silicon photonics to high performance computer networks are reviewed including sub-10fJ/bit modulator demonstrations, high-speed bandpass switches, tunable filters, actively stabilized resonators, and low dark current germanium detectors. Article not available.
Sequence Analysis and Optimal Matching Methods in Sociology (2000)

The authors review all known studies applying optimal matching or alignment (OM) techniques to social science sequence data. Issues of data, coding, temporality, cost setting/algorithm design, and analytic strategies are considered, and substantive findings are reviewed. The authors conclude that OM techniques have produced interesting results in a wide variety of areas, the most promising being studies of careers and of sequentially organized cultural artifacts.
<title>Turbo recognition: a statistical approach to layout analysis</title> (2000)

Turbo recognition (TR) is a communication theory approach to the analysis of rectangular layouts, in the spirit of Document Image Decoding. The TR algorithm, inspired by turbo decoding, is based on a generative model of image production, in which two grammars are used simultaneously to describe structure in orthogonal (horizontal and vertical directions. This enables TR to strictly embody non-local constraints that cannot be taken into account by local statistical methods. This basis in finite state grammars also allows TR to be quickly retargetable to new domains. We illustrate some of the capabilities of TR with two examples involving realistic images. While TR, like turbo decoding, is not guaranteed to recover the statistically optimal solution, we present an experiment that demonstrates its ability to produce optimal or near-optimal results on a simple yet nontrivial example, the recovery of a filled rectangle in the midst of noise. Unlike methods such as stochastic context free grammars and exhaustive search, which are often intractable beyond small images, turbo recognition scales linearly with image size, suggesting TR as an efficient yet near-optimal approach to statistical layout analysis.© (2000) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
PP.29.11 (2015)

Objective: To assess the relation of the education level in prevalence, awareness, treatment and control of hypertension in Colombia. Design and method: Cross-sectional study with longitudinal component. All participants were selected from urban and rural communities within 11 of Colombia[Combining Acute Accent]s departments. Households were eligible if at least one member was 35 to 70 years-old and if the members intended to continue living at that address for another 4 years or more. Data was entered into the iDATAfax® system. Data analysis was performed by using STATA/SE 12. Results: A total of 21390 individuals were selected for the survey. Of these, 7444 individuals aged 35 to 70 years were eligible from 58 communities, of which 49.5% were in rural areas and 64% were female. The mean age was 50.81 ± 9.75 years-old. 21.9% never attended school and 33.9% had a high-school or higher level of education. The prevalence of hypertension (HTA) was 36.7% [95% CI: 35.6 - 37.8%]. The highest prevalence of HTA was in the Pacific region 40.5% and the lowest was in the Amazonian region 25.6% (p < 0.001). The prevalence, awareness, treatment and control of HTA showed significant trends by educational level (p < 0.001) (Figure). Higher education was associated with a lower prevalence of HTA in younger and older age groups (p = 0.005), women (p < 0.001) but no men, urban and rural (p < 0.001) and in specific regions (Atlantic, Central, Amazonian, p < 0.05). Higher education was associated with awareness, treatment and control in men and rural areas only (p < 0.05).Conclusions: Hypertension is highly prevalent in Colombia as previously reported in other Latin America countries. The low level of awareness, treatment and control is related with low educational level and rural communities. The significant regional differences in these factors, suggests the need to develop region-specific strategies to achieve the desired improvements in detection and control.
Amine Dealkylations with Acyl Chlorides (1989)

The use of cyanogen bromide to dealkylate tertiary amines is known as the von Braun reaction. More recently, the development of carbonochloridate (chloroformate) reagents to effect these N-dealkylations has reached such high yields and mild reaction conditions as to open up the possibility of using simple alkyl groups to protect secondary amines. If an acyl chloride moiety is introduced within the molecule containing the tertiary amine, either by reaction of an organic acid with thionyl chloride or by treatment of a diamine with phosgene, intramolecular cyclization-N-dealkylation results leading to novel products. 1. Introduction 2. Kinetic and Mechanistic Aspects 3. Isolation of the Intermediate Salts 4. N-Dealkylations 5. Cyclization-Dealkylation Reactions 6. Summary and Outlook
Augmented expression of atrial natriuretic polypeptide gene in ventricle of human failing heart. (1989)

To elucidate the expression of the atrial natriuretic polypeptide (ANP) gene in the ventricle of the human failing heart, we have measured ANP and ANP messenger RNA (ANPmRNA) levels in left ventricular aneurysm obtained at operation, biopsy specimens of left ventricles from dilated cardiomyopathy (DCM) and autopsy samples of old myocardial infarction (OMI) and DCM hearts, and compared the levels with those in the normal ventricle. The ANP level (mean +/- SE) was 17.5 +/- 6.9 ng/g in the normal ventricle, and increased to 660.3 +/- 122.2 ng/g in the left ventricular aneurysm tissues and to 3,138.6 +/- 1,642.1 ng/g in the biopsy specimens of the DCM ventricle. These levels were approximately 40 and 200 times higher than in the normal ventricle. The increase of ANP levels was observed in both infarcted and noninfarcted regions of the OMI heart, and in the entire ventricle of the DCM heart. A significant positive correlation was found between the ANP level in aneurysm tissues and pulmonary capillary wedge pressure (r = 0.85). The ANPmRNA level in the left ventricular aneurysm showed about a 10-fold increase compared with that in the normal heart and reached 23% of that in the atrium of the same heart. A similar increase in the ANPmRNA level was observed in the entire ventricle of DCM. These data clearly indicate that the expression of the ANP gene in the ventricle is augmented in the failing heart in accordance with the severity of heart failure. In the atrium of the failing heart, ANP and ANPmRNA levels were only two times higher than those in the normal atrium. Thus, the augmentation in the expression of the ANP gene was more prominent in the ventricle than in the atrium. Taking tissue weight into account, the total content of ANPmRNA in the ventricle of the failing heart is much the same as that in the normal atrium. The ratio of the ANP level to the ANPmRNA level in the ventricle is much smaller than that in the atrium. These results suggest more rapid secretion of ANP after synthesis in the ventricle. These findings demonstrate that the expression of the ANP gene is augmented in the human ventricle of the failing heart and suggest that the ventricle becomes a substantial source of circulating ANP in congestive heart failure.