gebhart/patrepeval huggingface dataset:
Theory of the Air-Supported Puck (1964)

An approximate solution of the Navier-Stokes equation is given for incompressible flow through the thin annular gap beneath an air-supported puck. Expressions are derived which relate the pressure in the air film to the weight and dimensions of the puck, the thickness of the film to the total air flow, and the retarding force to the speed of a moving puck.
Cis-effects of heterochromatin on heterochromatic and euchromatic gene activity in Drosophila melanogaster. (1995)

Chromosomal rearrangements that juxtapose heterochromatin and euchromatin can result in mosaic inactivation of heterochromatic and euchromatic genes. This phenomenon, position effect variegation (PEV), suggests that heterochromatic and euchromatic genes differ in their regulatory requirements. This report describes a novel method for mapping regions required for heterochromatic genes, and those that induce PEV of a euchromatic gene. P transposase mutagenesis was used to generate derivatives of a translocation that variegated for the light+ (lt+) gene and carried the euchromatic white+ (w+) gene on a transposon near the heterochromatin-euchromatin junction. Cytogenetic and genetic analyses of the derivatives showed that P mutagenesis resulted in deletions of several megabases of heterochromatin. Genetic and molecular studies showed that the derivatives shared a euchromatic breakpoint but differed in their heterochromatic breakpoint and their effects on seven heterochromatic genes and the w+ gene. Heterochromatic genes differed in their response to deletions. The lt+ gene was sensitive to the amount of heterochromatin at the breakpoint but the heterochromatic 40Fa gene was not. The severity of variegated w+ phenotype did not depend on the amount of heterochromatin in cis, but varied with local heterochromatic environment. These data are relevant for considering mechanisms of PEV of both heterochromatic and euchromatic genes.
Professional Antigen-Presentation Function by Human γδ T Cells (2005)

Human gammadelta T cells are considered to play a vital role in protective immunity through cytokine secretion and cytotoxic activity. We report that cells expressing the Vgamma2Vdelta2+-T cell receptor (Vdelta2+ T cells) also display principal characteristics of professional antigen-presenting cells such as dendritic cells. Thus, when activated, these cells efficiently processed and displayed antigens and provided co-stimulatory signals sufficient for strong induction of naïve alphabeta T cell proliferation and differentiation. We suggest that, upon microbial activation, Vdelta2+ T cells participate in the induction of adaptive immune responses and that these cells may be a useful tool in vaccine development and immunotherapy.
Molecular characterization of the pan-B cell antigen CDw78 as a MHC class II molecule by direct expression cloning of the transcription factor CIITA (1995)

Antibodies against a pan-B cell-surface marker have been statistically clustered to define the CDw78 antigen. Molecular characterization of the CDw78 antigen was performed to determine its functional role in T and B cell interactions. Screening a direct expression cDNA library, made from the Epstein-Barr virus-transformed, B lymphoblastoid cell line MP-1 with an anti-CDw78 mAb, we have isolated a 3.8 kb cDNA encoding the transcription factor CIITA, previously shown to transactivate human MHC class II expression. Using several anti-CD78 mAb, we observed induced expression of the CDw78 antigen on the surface of monkey CV-1/EBNA cells transfected with the CIITA cDNA clone. Apparently, recombinant human CIITA can regulate the expression of monkey MHC class II which is recognized by anti-human CDw78 mAb. The anti-human CDw78 mAb seem to recognize a monomorphic determinant of MHC class II. Expression of recombinant human MHC class II antigens in CV-1/EBNA cells was recognized by a panel of CDw78 mAb, as well as by anti-human MHC class II mAb. These data show that the CDw78 antigen is expressed as part of the MHC class II molecule and appears similar if not identical to the alpha beta heterodimeric MHC class II antigen.
Ligand for peroxisome proliferator-activated receptor gamma (troglitazone) has potent antitumor effect against human prostate cancer both in vitro and in vivo. (1998)

Troglitazone, a thiazolidinedione derivative, is a widely used antidiabetic drug that binds and activates peroxisome proliferator-activated receptor gamma (PPARgamma) and enhances insulin sensitivity. It induces differentiation of adipocytes, which highly express PPARgamma. We report that human prostate cancer cells expressed PPARgamma at prominent levels and normal prostate tissues had very low expression. Dose-response clonogenic assays of the PC-3 prostate cancer cell line with troglitazone showed an antiproliferative effect (ED50, 3 x 10(-7) M) and other PPARgamma ligands (BRL49653: ED50, 8 x 10(-8) M; 15-deoxy-delta12,14-prostaglandin J2: ED50, 2 x 10(-6) M; ciglitizone: ED50, not reached; indomethacin: ED50, not reached) showed similar effects. Combinations of troglitazone and a ligand specific for either retinoid X receptor or retinoic acid receptor did not show a synergistic effect. Pulse-exposure to troglitazone (10(-5) M) for different durations showed that 4 days of pulse-exposure to the agent irreversibly inhibited 50% clonal growth of PC-3 cells. Interestingly, PC-3 cells cultured with troglitazone (10(-5) M) showed dramatic morphological changes both by light and electron microscopy, suggesting that the cells became less malignant. Nevertheless, troglitazone did not affect either the cell cycle or several markers of differentiation. LNCaP cells constitutively produced prostate-specific antigen, and levels were markedly enhanced by all-trans-retinoic acid. Troglitazone (10(-5) M, 4 days) decreased by 50% the levels of prostate-specific antigen produced by these cells. In vivo treatment of PC-3 tumors growing in male BNX triple immunodeficient mice with oral troglitazone (500 mg/kg/day) produced significant inhibition of tumor growth (P = 0.01). The only objective side effect of troglitazone in mice was the elevation of serum transaminases. Short-term culture of four surgically obtained human prostate cancer tumors with troglitazone (10(-5) M, 4 days) produced marked and selective necrosis of the cancer cells (about 60%) but not the adjacent normal prostate cells. Taken together, these results suggest that troglitazone may be a useful therapeutic agent for the treatment of prostate cancer, especially in the setting of low disease burden.
Electrochemotherapy, a new antitumor treatment. First clinical phase I-II trial (1993)

The absence of toxicity, the good tolerance by the patients, and the net antitumor effects observed are encouraging for additional electrochemotherapy developments in clinical oncology.
CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans (2013)

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
La doble dimensión de la vida en los derechos humanos: como fundamento y como Derecho (2014)

Analizar críticamente el derecho a la vida es importante debido a que frecuentemente se le considera como el fundamento de los demás derechos. No obstante, de igual manera el derecho a la vida suele abordarse de una manera simplificada, reduciendo “la vida” como derecho tan sólo al momento de su inicio y al momento de su final, soslayando su carácter procesual que significa comprender la vida como producción, reproducción y desarrollo. Así, por ejemplo, se utiliza el “derecho a la vida” de manera ideologizada en discusiones relacionadas con el aborto y la eutanasia, contraponiendo “vida” a “libertad”. Este uso del derecho a la vida puede considerársele como conservador, por su carácter idealista, pues excluye de su discurso las condiciones materiales necesarias para hacer viable una vida digna e integral. La estrategia discursiva de colocar como fundamento de todos los derechos el derecho a la vida conduce habitualmente a contraponerlo con otros derechos, como si la vida no tuviera qué vivirse libremente, con igualdad de trato, en comunicación con los demás, con vivienda y salud, con trabajo y educación, etc.
Using gene expression profiling to identify the molecular basis of the synergistic actions of hepatocyte growth factor and vascular endothelial growth factor in human endothelial cells (2003)

Hepatocyte growth factor (HGF) and vascular endothelial cell growth factor (VEGF) are two potent endothelial mitogens with demonstrated angiogenic activities in animal models of therapeutic angiogenesis. Several recent studies suggest that these growth factors may act synergistically, although the mechanism of this interaction is not understood. Changes in the gene expression profile of human umbilical vein endothelial cells treated with HGF, VEGF or the combination of the two were analyzed with high-density oligonucleotide arrays, representing approximately 22000 genes. Notably, the genes significantly up- and downregulated by VEGF versus HGF exhibited very little overlap, indicating distinct signal transduction pathways. The combination of HGF and VEGF markedly increased the number of significantly up- and downregulated genes. At 4 h, the combination of the two growth factors induced a number of chemokine and cytokines and their receptors (IL-8, IL-6, IL-11, CCR6, CXCR1,CXC1 and IL17RC), numerous genes involved in growth factor signal transduction (egr-1, fosB, grb10, grb14,MAP2K3,MAP3K8, MAPKAP2,MPK3, DUSP4 and DUSP6), as well as a number of other growth factors (PDGFA, BMP2, Hb-EGF, FGF16, heuregulin beta 1, c-kit ligand, angiopoietin 2 and angiopoietin 4 and VEGFC). In addition, the VEGF receptors neuropilin-1 and flt-1 were also upregulated. At 24 h, a clear 'cell cycle' signature is noted, with the upregulated expression of various cell cycle control proteins and gene involved in the regulation of mitosis and mitotic spindle assembly. The receptor for HGF, c-met, is also upregulated. These data are consistent with the hypothesis that the combination of HGF and VEGF results in the cooperative upregulation of a number of different molecular pathways leading to a more robust proliferative response, that is, growth factor(s), receptors, molecules involved in growth factor signal transduction, as well as, at later time points, upregulation of the necessary cellular proteins required for cells to escape cell cycle arrest and enter the cell cycle.
Marker-specific sorting of rare cells using dielectrophoresis (2005)

Current techniques in high-speed cell sorting are limited by the inherent coupling among three competing parameters of performance: throughput, purity, and rare cell recovery. Microfluidics provides an alternate strategy to decouple these parameters through the use of arrayed devices that operate in parallel. To efficiently isolate rare cells from complex mixtures, an electrokinetic sorting methodology was developed that exploits dielectrophoresis (DEP) in microfluidic channels. In this approach, the dielectrophoretic amplitude response of rare target cells is modulated by labeling cells with particles that differ in polarization response. Cell mixtures were interrogated in the DEP-activated cell sorter in a continuous-flow manner, wherein the electric fields were engineered to achieve efficient separation between the dielectrophoretically labeled and unlabeled cells. To demonstrate the efficiency of marker-specific cell separation, DEP-activated cell sorting (DACS) was applied for affinity-based enrichment of rare bacteria expressing a specific surface marker from an excess of nontarget bacteria that do not express this marker. Rare target cells were enriched by >200-fold in a single round of sorting at a single-channel throughput of 10,000 cells per second. DACS offers the potential for automated, surface marker-specific cell sorting in a disposable format that is capable of simultaneously achieving high throughput, purity, and rare cell recovery.
`Hass' Avocado Carbohydrate Fluctuations. II. Fruit Growth and Ripening (1999)

Changes in soluble sugar and starch reserves in avocado ( Persea americana Mill. on `Duke 7' rootstock) fruit were followed during growth and development and during low temperature storage and ripening. During the period of rapid fruit size expansion, soluble sugars accounted for most of the increase in fruit tissue biomass (peel: 17% to 22%, flesh: 40% to 44%, seed: 32% to 41% of the dry weight). More than half of the fruit total soluble sugars (TSS) was comprised of the seven carbon (C7) heptose sugar, D-mannoheptulose, and its polyol form, perseitol, with the balance being accounted for by the more common hexose sugars, glucose and fructose. Sugar content in the flesh tissues declined sharply as oil accumulation commenced. TSS declines in the seed were accompanied by a large accumulation of starch (≈30% of the dry weight). During postharvest storage at 1 or 5 °C, TSS in peel and flesh tissues declined slowly over the storage period. Substantial decreases in TSS, and especially in the C7 sugars, was observed in peel and flesh tissues during fruit ripening. These results suggest that the C7 sugars play an important role, not only in metabolic processes associated with fruit development, but also in respiratory processes associated with postharvest physiology and fruit ripening.
A novel image-based rendering method by linear filtering of multiple focused images acquired by a camera array (2004)

In this paper, we present a novel approach to image-based rendering (IBR) for generating an arbitrary view image with arbitrary focus for a scene consisting two approximately constant depths. The presented method differs from the conventional IBRs using multiple view images in that we acquire two differently focused images from each camera position and render parallax and focus effects on objects at different depth simply by linear filtering of the acquired images without segmentation. Experimental results on the real images acquired with 4 cameras located in parallel are presented.
Predicting eye fixations using convolutional neural networks (2015)

It is believed that eye movements in free-viewing of natural scenes are directed by both bottom-up visual saliency and top-down visual factors. In this paper, we propose a novel computational framework to simultaneously learn these two types of visual features from raw image data using a multiresolution convolutional neural network (Mr-CNN) for predicting eye fixations. The Mr-CNN is directly trained from image regions centered on fixation and non-fixation locations over multiple resolutions, using raw image pixels as inputs and eye fixation attributes as labels. Diverse top-down visual features can be learned in higher layers. Meanwhile bottom-up visual saliency can also be inferred via combining information over multiple resolutions. Finally, optimal integration of bottom-up and top-down cues can be learned in the last logistic regression layer to predict eye fixations. The proposed approach achieves state-of-the-art results over four publically available benchmark datasets, demonstrating the superiority of our work.
Correction of ultrasonic wavefront distortion using backpropagation and a reference waveform method for time-shift compensation (1994)

A model is introduced to describe ultrasonic pulse amplitude and shape distortion as well as arrival time fluctuation produced by propagation through specimens of human abdominal wall. In the model, amplitude and shape distortion develops as the wavefront propagates in a uniform medium after passing through a phase screen that only causes time shifts. This distortion is compensated by a backpropagation of the wavefront using the angular spectrum method. The compensation employed waveforms emitted by a pointlike source and measured after propagation through the tissue. The waveforms were first corrected for geometric path and then were backpropagated over a sequence of increasing distances. At each distance, a waveform similarity factor was calculated to find the backpropagation distance at which the waveforms were most similar. A new method was devised to estimate pulse arrival time for geometric correction as well as to perform time-shift compensation. The method adaptively derives a reference waveform that is then cross correlated with all the waveforms in the aperture to obtain a surface of arrival times. The surface was smoothed iteratively to remove outlying points due to waveform distortion. The mean (±s.d.) of the waveform similarity factor for 14 specimens was found to be 0.938 (±0.025) initially. After backpropagation of waveforms to the distance of maximum waveform similarity for each specimen, the waveform similarity factor improved to 0.967 (±0.015). The corresponding energy level fluctuation in the wavefront was 4.2 (±0.4) dB initially and became 3.3 (±0.3) dB after backpropagation. For wavefronts focused at 180 mm, the −30 dB mean (±s.d.) effective radius of the focus was 4.2 (±1.2) mm with time-shift compensation in the aperture and became 2.5 (±0.5) mm with backpropagation followed by time-shift compensation. These results indicate that a phase screen placed some distance away from the aperture is an improved model for the description of wavefront distortion produced by human abdominal wall and that wavefront backpropagation followed by time-shift estimation and compensation is an effective method to compensate for such distortion.
A 1 Mb/s digital subscriber line transceiver signal processor (1993)

A monolithic custom digital signal processor which satisfies both the US and the European standards for HDSL (high-bit-rate digital subscriber line) transmission is presented. The architecture of an HDSL transceiver is shown. At the receiver, the ADC (analog-to-digital converter) samples the signal once per symbol period. The transmit energy is removed using an echo canceller. The resulting receive energy is then prefiltered and sent to the feedforward equalizer (FFE). The FFE provides gain and removes any precursor intersymbol interference (ISI). The decision-feedback equalizer then removes the postcursor ISI. The resulting data are sliced into a symbol decision and error value.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Highly Efficient and Accelerated Suzuki Aryl Couplings Mediated by Phosphine-Free Palladium Sources (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHighly Efficient and Accelerated Suzuki Aryl Couplings Mediated by Phosphine-Free Palladium SourcesThomas I. Wallow and Bruce M. NovakCite this: J. Org. Chem. 1994, 59, 17, 5034–5037Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/jo00096a056https://doi.org/10.1021/jo00096a056research-articleACS PublicationsRequest reuse permissionsArticle Views2961Altmetric-Citations242LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Identification and characterization of antigenic determinants of Mycobacterium leprae that react with antibodies in sera of leprosy patients (1990)

Antigenic determinants of Mycobacterium leprae were identified by screening a lambda gt11::M. leprae genomic library with two separate pools of sera from leprosy patients. A total of 45 recombinant clones were detected with pooled sera from 21 lepromatous (LL) leprosy patients and 5 additional clones specified polypeptides that reacted with antibodies in pooled sera from 30 borderline tuberculoid or tuberculoid leprosy patients. The recombinant clones that specified antigenic determinants that reacted with sera from LL patients were condensed into eight groups on the basis of DNA hybridization experiments among the M. leprae DNA insert fragments. In addition, 11 of the 45 recombinant clones did not hybridize to members of the eight groups nor to one another; these represent unique recombinant clones. None of the recombinant clones identified by screening with sera from tuberculoid leprosy patients hybridized to each other or to any of the 45 LL recombinant clones. The polypeptides specified by the recombinant clones were usually fusion proteins with beta-galactosidase, ranging in size from 117 to 175 kilodaltons (kDa). Members of hybridization group III specified nonfusion proteins of 45 kDa. Only members of hybridization group I reacted with any of 30 monoclonal antibodies prepared against M. leprae proteins; recombinant proteins from these clones reacted with a single monoclonal antibody directed against the M. leprae 65-kDa protein. Thus, at least 22 new antigenic determinants of M. leprae have been identified on the basis of their reactivity to antibodies in sera from LL patients or sera from tuberculoid leprosy patients or both.
Synthesis of β-amino-sulphones and αβ-unsaturated sulphones (1954)

M. Balasubramanian and V. Baliah, J. Chem. Soc., 1954, 1844 DOI: 10.1039/JR9540001844
Nicotine inhibits myofibroblast differentiation in human gingival fibroblasts (2005)

Cigarette smoking has been suggested as a risk factor for several periodontal diseases. It has also been found that smokers respond less favorably than non-smokers to periodontal therapy. Previous work in our lab has shown that nicotine inhibits human gingival cell migration. Since myofibroblasts play an important role in wound closure, we asked if nicotine affects gingival wound healing process by regulating myofibroblast differentiation. Human gingival fibroblasts (HGFs) from two patients were cultured in 10% fetal bovine serum cell culture medium. Cells were pretreated with different doses of nicotine (0, 0.01, 0.1, and 1 mM) for 2 h, and then incubated with transforming growth factor beta (TGF-beta1) (0, 0.25, 0.5, and 1 ng/ml) with or without nicotine for 30 h. The expression level of alpha-smooth muscle actin (alpha-SMA), a specific marker for myofibroblasts, was analyzed by Western blots, immunocytochemistry, and real-time polymerase chain reaction (real-time PCR). Phosphorylated p38 mitogen-activated protein kinase (Phospho-p38 MAPK) activity was analyzed by Western blots. TGF-beta1 induced an increase of alpha-SMA protein and mRNA expression, while nicotine (1 mM) inhibited the TGF-beta1-induced expression of alpha-SMA but not beta-actin. Nicotine treatment down-regulated TGF-beta1-induced p38 MAPK phosphorylation. Our results demonstrated for the first time that nicotine inhibits myofibroblast differentiation in human gingival fibroblasts in vitro; supporting the hypothesis that delayed wound healing in smokers may be due to decreased wound contraction by myofibroblasts.