gebhart/patrepeval huggingface dataset:
Xylan utilization in human gut commensal bacteria is orchestrated by unique modular organization of polysaccharide-degrading enzymes (2014)

Enzymes that degrade dietary and host-derived glycans represent the most abundant functional activities encoded by genes unique to the human gut microbiome. However, the biochemical activities of a vast majority of the glycan-degrading enzymes are poorly understood. Here, we use transcriptome sequencing to understand the diversity of genes expressed by the human gut bacteria Bacteroides intestinalis and Bacteroides ovatus grown in monoculture with the abundant dietary polysaccharide xylan. The most highly induced carbohydrate active genes encode a unique glycoside hydrolase (GH) family 10 endoxylanase (BiXyn10A or BACINT_04215 and BACOVA_04390) that is highly conserved in the Bacteroidetes xylan utilization system. The BiXyn10A modular architecture consists of a GH10 catalytic module disrupted by a 250 amino acid sequence of unknown function. Biochemical analysis of BiXyn10A demonstrated that such insertion sequences encode a new family of carbohydrate-binding modules (CBMs) that binds to xylose-configured oligosaccharide/polysaccharide ligands, the substrate of the BiXyn10A enzymatic activity. The crystal structures of CBM1 from BiXyn10A (1.8 Å), a cocomplex of BiXyn10A CBM1 with xylohexaose (1.14 Å), and the CBM from its homolog in the Prevotella bryantii B14 Xyn10C (1.68 Å) reveal an unanticipated mode for ligand binding. A minimal enzyme mix, composed of the gene products of four of the most highly up-regulated genes during growth on wheat arabinoxylan, depolymerizes the polysaccharide into its component sugars. The combined biochemical and biophysical studies presented here provide a framework for understanding fiber metabolism by an important group within the commensal bacterial population known to influence human health.
High frequency characterization of SOI dynamic threshold voltage MOS (DTMOS) transistors (2003)

The DTMOS architecture is particularly suited to very low supply voltage applications (0.5-0.6 V) (Colinge, 1987; Matloubian, 1993; Assaderaghi et al., 1994; Pelloie et al., 1999). This paper presents the high frequency behavior of DTMOS devices processed with a partially depleted 0.25 /spl mu/m SOI technology (Wilson et al., 1997; Lagnado and de la Houssaye, 1997; Cable, 1997; Ferlet-Cavrois et al., 1998; Tanaka et al., 1997). The paper compares DTMOS to floating body and grounded body MOS transistors, and shows the advantage of SOI DTMOS for very low power portable telecommunication systems.
Phosphonitrilic compounds. XI. Electroreduction and electron spin resonance spectra of phosphazenes (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphonitrilic compounds. XI. Electroreduction and electron spin resonance spectra of phosphazenesH. R. Allcock and W. J. BirdsallCite this: Inorg. Chem. 1971, 10, 11, 2495–2499Publication Date (Print):November 1, 1971Publication History Published online1 May 2002Published inissue 1 November 1971https://pubs.acs.org/doi/10.1021/ic50105a025https://doi.org/10.1021/ic50105a025research-articleACS PublicationsRequest reuse permissionsArticle Views193Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Ste-Justine Adolescent Idiopathic Scoliosis Cohort Study (1994)

Comparisons on health outcomes between the two study groups should not be affected by differential patterns of selection or response.
Organizações Familiares: Estudo de Caso da Malharia Maxxi Malhas (2015)

entre os respondentes, pois estão adaptando-se aos novos acontecimentos.
Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors (2012)

Circulating tumor cells (CTCs) have been implicated as a population of cells that may seed metastasis and venous thromboembolism (VTE), two major causes of mortality in cancer patients. Thus far, existing CTC detection technologies have been unable to reproducibly detect CTC aggregates in order to address what contribution CTC aggregates may make to metastasis or VTE. We report here an enrichment-free immunofluorescence detection method that can reproducibly detect and enumerate homotypic CTC aggregates in patient samples. We identified CTC aggregates in 43% of 86 patient samples. The fraction of CTC aggregation was investigated in blood draws from 24 breast, 14 non-small cell lung, 18 pancreatic, 15 prostate stage IV cancer patients and 15 normal blood donors. Both single CTCs and CTC aggregates were measured to determine whether differences exist in the physical characteristics of these two populations. Cells contained in CTC aggregates had less area and length, on average, than single CTCs. Nuclear to cytoplasmic ratios between single CTCs and CTC aggregates were similar. This detection method may assist future studies in determining which population of cells is more physically likely to contribute to metastasis and VTE.
A liposomal MUC1 vaccine for treatment of non-small cell lung cancer (NSCLC): Updated survival results from patients with stage IIIB disease (2005)

7037 Background: The primary analysis results from a randomized, open label phase IIb trial utilizing L-BLP25 (BLP25 Liposome Vaccine) for the immunotherapy of stage IIIB and IV NSCLC patients with stable or responding disease following any first line chemotherapy were reported in April 2004. Patients were randomized 1:1 to best supportive care (BSC) or BSC + L-BLP25 (TX), with survival as the primary endpoint. Treatment arm patients received a single intravenous dose of 300mg/m2 cyclophosphamide, followed by 8 weekly subcutaneous immunizations with L-BLP25. Maintenance immunizations are then given at 6-week intervals. At primary analysis, the median survival of TX patients in the pre-stratified stage IIIB locoregional (LR) disease subset, had not yet been reached. Methods: Updated survival information was obtained as part of a protocol specified follow-up period at two years after enrollment of the last patient. Survival was calculated from the date of randomization into the trial, to the date of death or last contact. Results: Of 171 patients enrolled, 65 had IIIB LR disease. Of these, 35 were randomized to TX, and 30 to BSC. The IIIB LR subgroup was well balanced in terms of age and ethnicity. More female and ECOG 0 patients were randomized to TX than BSC (51.4 versus 36.7%, and 40.0 versus 26.7%), and more patients in the TX arm received radiotherapy in addition to chemotherapy, for cancer treatment prior to trial enrollment (91.4 versus 76.7%). The current median number of vaccinations received by TX patients is 17, with a maximum of 41. Mild injection site reactions occurred in 54.3% of patients. There were no major toxicities. To date, a survival median for TX patients has not yet been reached (54% alive at >24 months). Conclusion: A clinically meaningful survival advantage appears likely for patients with stage IIIB NSCLC treated with L-BLP25. Further development of the vaccine is warranted. Author Disclosure Employment or Leadership Consultant or Advisory Role Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Biomira, Roche Aventis, Biomira, Roche
Interactive virtual materials (2004)

In this paper we present a fast and robust approach for simulating elasto-plastic materials and fracture in real time. Our method extends the warped stiffness finite element approach for linear elasticity and combines it with a strain-state-based plasticity model. The internal principal stress components provided by the finite element computation are used to determine fracture locations and orientations. We also present a method to consistently animate and fracture a detailed surface mesh along with the underlying volumetric tetrahedral mesh. This multi-resolution strategy produces realistic animations of a wide spectrum of materials at interactive rates that have typically been simulated off-line thus far.
NFC-Enabled Wireless Charging (2012)

This paper summarizes the results of our concept development for NFC-enabled wireless charging - i.e., an add-on to the existing NFC operating modes that implements charging power transmission and charging power reception with associated control functionality. When compared to dedicated wireless charging solutions, integration of NFC and wireless charging into the same implementation will entail more compact and cost-efficient charging interfaces for portable devices, a more pervasive charger infrastructure in future with reduced costs, and the possibility of integrating NFC-based services into charging applications. We propose to the NFC community a closer investigation of use cases, the business potential, and technical implementation of NFC-enabled charging. This should lead to a road map for its wider adoption, pave the way to its standardization, technology development, and commercial application, and also support enhancement of power transfer capability in other NFC applications alongside wireless charging.
Remote telesurgical mentoring: feasibility and efficacy (2005)

We report our experience in performing telesurgically-mentored procedures from Baltimore, MD to Thailand, Austria, Italy and Singapore. We additionally report on a case of remote robotic manipulation using a robot designed for Percutaneons Access of the Kidney (PAKY). Telementoring was performed using a video teleconferencing platform including audio, video, real-time video telestration and remote control of the AESOP robotic arm that manipulates the laparoscopic camera. The international telementoring was achieved over 3- ISDN lines (384 Kilobytes/sec). The robotic arm and PAKY robot were controlled over a separate analog POTS line. Telecommunications links were successfully established to these remote locations that ranged in distance from approximately 4,500-11,000 miles. There was no perceptible impact of time delay on the surgical procedures. All procedures were successfully completed without additional complications. Multiple laparoscopic surgeries to include varicocelectomy (4), nephrectomy (4), adrenalectomy (1) and cholecystectomies (2) were demonstrated. Remote robotic percutaneous access to the kidney was successfully conducted within fifteen minutes from Baltimore, Maryland to Rome, Italy.
Capillary gel electrophoresis for rapid, high resolution DNA sequencing (1990)

Capillary gel electrophoresis has been demonstrated for the separation and detection of DNA sequencing samples. Enzymatic dideoxy nucleotide chain termination was employed, using fluorescently tagged oligonucleotide primers and laser based on-column detection (limit of detection is 6,000 molecules per peak). Capillary gel separations were shown to be three times faster, with better resolution (2.4 x), and higher separation efficiency (5.4 x) than a conventional automated slab gel DNA sequencing instrument. Agreement of measured values for velocity, resolution and separation efficiency with theory, predicts further improvements will result from increased electric field strengths (higher voltages and shorter capillaries). Advantages of capillary gel electrophoresis for automatic DNA sequencing instruments and for genomic sequencing are discussed.
Automatic Detection of Retina Layers using Texture Analysis (2012)

In this paper, two computer approaches is proposed for recognition of retina layers on optical coherence tomography (OCT) images. OCT uses the optical backscattering of light to scan the eye and describe a pixel representation of the anatomic layers within the retina. Our approaches is based on co-occurrence matrix for feature extraction and a neural network and a supervised learning method for classification, which four features of this matrix have been selected as a feature vector by support vector machine (SVM) and multilayer perceptron (MLP) have been used for classifying retina layers. Achieved results of combined these methods in the best state was 96.6% precision by MLP and 98.6% by SVM method. These results show that apply these methods on OCT images discriminate retina layers with efficient accuracy. Since, recognition of retina layers is important for automatic analyzing of OCT images, therefore our proposed methods can be very useful.
High-throughput SNP genotyping with the Masscode system. (2000)

QIAGEN Genomics, Inc, has developed the Masscode tagging system for DNA labeling and detection. In this application, the Masscode system is described as applied to high-throughput single-nucleotide polymorphism (SNP) genotyping. The labeling system is based on a small-molecular-weight tag that is covalently attached through a photocleavable linker to a DNA oligonucleotide. The tagged oligonucleotide is used as a primer in an allele-specific PCR SNP discrimination assay. The allele-specific amplicons are differentiated through their Masscode tag assignments. After a photolysis step to cleave the tags from the amplicon, the samples are introduced into a single quadrupole mass spectrometry detection system for analysis. Genotyping determinations are based on the relative proportions of the paired allele tags. The system has a lower limit of detection in the femtomolar range (10(-15) M). At present, 30 different Masscode tags may be used simultaneously in a multiplex fashion to routinely provide more than 40,000 SNP genotyping measurements daily. Further developments will allow for the simultaneous detection of several hundred tags.
Binding Site of Cerulenin in Fatty Acid Synthetase1 (1989)

An antibiotic cerulenin, (2R, 3S)-2,3-epoxy-4-oxo-7,10-trans,trans- dodecadienamide, irreversibly inhibits fatty acid synthetase from Saccharomyces cerevisiae. Three moles of cerulenin were bound to 1 mol of the enzyme with concomitant loss of its activity. Pretreatment of the enzyme with iodoacetamide reduced the amount of cerulenin bound to the enzyme. Since iodoacetamide is known to specifically bind to the cysteine residue on the condensing reaction domain, cerulenin is considered to bind to the same domain. Tryptic digestion of the [3H] cerulenin-treated enzyme gave a radioactive peptide; its amino acid composition was Asx 1, Thr 1, Ser 1, Glx 2, Pro 1, Gly 1, Ala 1, Val 1, Ile 1, and Leu 2. This composition included all the amino acids of the condensing reaction site (Thr-Pro-Val-Gly-Ala-Cys) previously reported by Kresze et al. (Eur. J. Biochem., 79, 181 [1977] except for Cys. When the enzyme was treated with [3H]cerulenin and digested successively with trypsin and carboxypeptidase P, a [3H] cerulenin-cysteine adduct was isolated as the sole product. This was identified with the adduct chemically synthesized from non-labeled cerulenin and cysteine, and its structure was elucidated by 1H-, 13C-NMR, and fast atom bombardment mass spectrometry. These results indicate that cerulenin, forming a hydroxylactam ring, reacts at its epoxide carbon (C-2 position) with the SH-group of the cysteine residue in the condensing reaction domain of yeast fatty acid synthetase.
Current Role of Platelet Glycoprotein IIb/IIIa Inhibitors in Acute Coronary Syndromes (2000)

Current data indicate that intravenous Gp IIb/IIIa inhibitor therapy merits a prominent role in the initial management of patients with ACSs. JAMA. 2000;284:1549-1558.
Transforming growth factor-β and breast cancer: Mammary gland development (2000)

Transforming growth factor (TGF)-beta1 is a pluripotent cytokine that profoundly inhibits epithelial proliferation, induces apoptosis, and influences morphogenesis by mediating extracellular matrix deposition and remodeling. The physiologic roles of the action of TGF-beta in mammary gland, indeed in most tissues, are poorly understood. In order to understand the actions of TGF-beta, we need to take into account the complexity of its effects on different cell types and the influence of context on cellular responses. This task is further compounded by multiple mechanisms for regulating TGF-beta transcription, translation, and activity. One of the most significant factors that obscures the action of TGF-beta is that it is secreted as a stable latent complex, which consists of the 24-kDa cytokine and the 80-kDa dimer of its prepro region, called latency-associated peptide. Latency imposes a critical restraint on TGF-beta activity that is often overlooked. The extracellular process known as activation, in which TGF-beta is released from the latent complex, is emphasized in the present discussion of the role of TGF-beta in mammary gland development. Definition of the spatial and temporal patterns of latent TGF-beta activation in situ is essential for understanding the specific roles that TGF-beta plays during mammary gland development, proliferation, and morphogenesis.
The clinical spectrum of cerebral amyloid angiopathy (1993)

Cerebral amyloid angiopathy is a common cause of spontaneous lobar hemorrhage in elderly patients. We discuss seven patients with amyloid angiopathy presenting without major lobar hemorrhage. The patients' presentations fell into two groups: recurrent transient neurologic symptoms and rapidly progressive dementia. The cases with transient episodes had a spread of symptoms to contiguous body areas during episodes. Each had evidence of small hemorrhage or subsequent large hemorrhage in the cortical location corresponding to the symptoms, suggesting petechial hemorrhage with focal seizure as the cause of the transient spells. Three cases of dementia developed with relatively rapid time courses, progressing from intact baseline to profound dementia in spans of a few days to 2 years. Pathologic abnormalities, in addition to amyloid angiopathy, included patchy white matter demyelination and tissue loss, petechial cortical hemorrhages, cortical infarctions, and a variable degree of neuritic plaques and neurofibrillary tangles. The clinical spectrum of cerebral amyloid angiopathy includes these two neurologic syndromes.
Distinct promoters regulate tissue‐specific and differential expression of kallikrein 6 in CNS demyelinating disease (2004)

Kallikrein 6 is a serine protease expressed abundantly in normal adult human and rodent CNS, and therein is regulated by injury. In the case of CNS demyelinating disease, K6 expression in CNS occurs additionally in perivascular and parenchymal inflammatory cells suggesting a role in pathogenesis. Herein we describe two unique transcripts that occur within the human and mouse K6 genes that differ in their 5'-untranslated regions. These transcripts have identical translation initiation sites in exon 3, are expressed in a tissue-specific fashion and are differentially regulated in response to CNS injury. While the human and mouse 5'-transcripts differ in sequence they are identical in genomic organization and tissue-specific expression. The most 5'-transcript, designated transcript 1, includes exon 1-7, and was detectable in all CNS regions, but not in any non-CNS tissues examined (spleen, thymus, liver, kidney, pancreas, submandibular gland and peripheral nerve). In contrast, transcript 2 lacks exon 1, but contains a unique sequence at the 5'-end of exon 2, designated exon 2A. Transcript 2 was expressed both in CNS and in each peripheral tissue. In a murine model of human CNS demyelinating inflammatory disease induced by Theiler's picornovirus, mouse K6 transcript 1 was up-regulated in brain and spinal cord at acute and more chronic phases of CNS inflammation and demyelination, while overall transcript 2 expression was not significantly altered. However, in isolated splenocyte cultures, transcript 2 was up-regulated two-fold by cellular activation. Tissue-specific expression patterns and differential regulation in CNS disease indicates that each K6 5'-transcript is probably regulated by unique promoter elements and may serve as a molecular target to treat inflammatory demyelinating disease.
Kinobeon A as a Potent Tyrosinase Inhibitor from Cell Culture of Safflower:In vitroComparisons of Kinobeon A with Other Putative Inhibitors (2003)

Kinobeon A is produced from cell cultures of the medicinal plant, safflower. Mushroom tyrosinase activity was inhibited in a concentration-dependent manner when treated with kinobeon A using L-tyrosine or L-3,4-dihydroxyphenylalannine (L-DOPA) as substrates. IC50 values were 22 microM (substrate: L-tyrosine) and 27 microM (L-DOPA). Inhibition of human tyrosinase activity also increased with increasing concentrations of kinobeon A using L-DOPA as the substrate, with an IC50 value of 2.5 microM. Kinobeon A was a more potent competitive inhibitor than kojic acid, arbutin or L-ascorbic acid for both mushroom and human tyrosinase as determined from Lineweaver-Burk plots. These results suggested that kinobeon A could be a potent natural tyrosinase inhibitor.
Fatigue microcrack detection with digital image correlation (2011)

A digital image correlation method is proposed to detect and quantify automatically microcracks on the surface of a specimen during a fatigue test. The proposed procedure allows for a fast scanning of the entire surface with all possible (pixel-wise) locations of microcrack centres and the detection of cracks having a sub-pixel opening. An experimental test case is presented as an illustration of the method and a comparison with a replica technique is performed.
Essential role of phosphatidylinositol 3-kinase in insulin-induced glucose transport and antilipolysis in rat adipocytes. Studies with a selective inhibitor wortmannin. (1994)

Significant activity of phosphatidylinositol 3-kinase (PI 3-kinase) was detected in the membrane fractions, or in the immunoprecipitates prepared with anti-phosphotyrosine antibodies, from rat adipocytes that had been incubated with insulin for 20 min. The PI 3-kinase activity in these preparations as well as in the whole cell lysates of adipocytes not treated with insulin was inhibited by the addition of wortmannin, a fungal metabolite, to the enzyme assay mixture. The inhibition was dependent on the inhibitor concentration with IC50 being less than 10 nM and perfect inhibition at 100 nM. The effect of insulin to induce membrane PI 3-kinase activity was mostly abolished, but its effects to tyrosine-phosphorylate the beta-subunit of the insulin receptor or other cellular substrate proteins including insulin-receptor-substrate-1 were not at all antagonized, by wortmannin added to the cell incubation medium. Insulin stimulation of cellular 2-deoxyglucose uptake and inhibition of isoproterenol-induced lipolysis observable in adipocytes under the same conditions were also antagonized by wortmannin added in the same concentration range as used for the inhibition of insulin-susceptible PI 3-kinase. It is concluded, therefore, that activation of wortmannin-sensitive PI 3-kinase plays a pivotal role in the intracellular signaling pathways arising from the insulin receptor autophosphorylation and leading to certain metabolic responses.