Therapeutic antibodies : methods and protocols (2009)

1 Therapeutic Antibodies: Current State and Future Trends - Is a Paradigm Change Coming Soon? Dimiter S. Dimitrov and James D. Marks PART I. Recombinant Antigens 2 Preparation of Recombinant Viral Glycoproteins for Novel and Therapeutic Antibody Discovery Yee-Peng Chan, Lianying Yan, Yan-Ru Feng, and Christopher C. Broder PART II. Antibody Libraries 3 Design of a Human Synthetic Combinatorial Library of Single-chain Antibodies Limor Nahary and Itai Benhar 4 Construction of a Human Antibody Domain (VH) Library Weizao Chen, Zhongyu Zhu, Xiaodong Xiao, and Dimiter S. Dimitrov 5 Generation and Selection of Rabbit Antibody Libraries by Phage Display Christoph Rader 6 Construction of a Large Naive Human Phage-Displayed Fab Library through One-Step Cloning Zhongyu Zhu and Dimiter S. Dimitrov PART III. Antibody Discovery 7 Identification of Target and Function Specific Antibodies for Effective Drug Delivery Yu Zhou and James D. Marks 8 Screening of Specific Internalization Fab Fragment from Human Naive Phage Library by Combinational Bio-Panning Xin Wang and Brain B. Cao 9 Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41 Mei-Yun Zhang and Dimiter S. Dimitrov 10 Selection of Non-Aggregating VH Binders from Synthetic VH Phage Display Libraries Mehdi Arbabi-Ghahroudi, Roger MacKenzie, and Jamshid Tanha 11 Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia Coli Yariv Mazor, Thomas Van Blarcom, Brent L. Iverson, and George Georgiou 12 Multiplexed Flow Cytometry: High-Throughput Screening of Single-ChainAntibodies Joanne Ayriss, Rosa Valero, Andrew R. M. Bradbury, and Peter Pavlik 13 Human Antibody Repertoires Per-Johan Meijer, Lars S. Nielsen, Johan Lantto, and Allan Jensen 14 Rapid Screening Platform for Stabilization of scFvs in E. coli Brian R. Miller, Scott M. Glaser, and Stephen J. Demarest PART IV. Antibody Engineering 15 In Vitro Antibody Affinity Maturation Targeting Germline Hotspots Mitchell Ho and Ira Pastan 16 Affinity Maturation by Phage Display Holger Thie, Bernd Voedisch, Stefan Dubel, Michael Hust, and Thomas Schirrmann 17 Production of Chimeric Heavy Chain Antibodies Jianbing Zhang, Roger MacKenzie, and Yves Durocher 18 Mammalian Cell Display for Antibody Engineering Mitchell Ho and Ira Pastan 19 Improving Antibody Binding Affinity and Specificity for Therapeutic Development Jenny Bostrom, Chingwei V. Lee, Lauric Haber, and Germaine Fuh 20 Construction and Production of an IgG-like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy Dan Lu and Zhenping Zhu 21 Deimmunisation of Monoclonal Antibodies Frank J. Carr, Tim D. Jones, Laura J. Crompton, and Matthew P. Baker 22 Anti-CD22 Onconase: Preparation and Characterization Dianne L. Newton, Luke H. Stockwin, and Susanna M. Rybak 23 Antibody-Cytotoxic Agent Conjugates: Preparation and Characterization Rajeeva Singh and Hans K. Erickson PART V. Antibody Preclinical Development 24 High-Level Production of a Humanized ImmunoRNase Fusion Protein from Stably Transfected Myeloma Cells Jurgen Krauss, Evelyn Exner, Athanasios Mavratzas, Siegfried Seeber, and Michaela A.E. Arndt 25 Antibody Fragment Expression and Purification Dimana Dimitrova, Vidita Choudhry, and Christopher