gebhart/patrepeval huggingface dataset:
A group electronegativity method with Pauling units (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA group electronegativity method with Pauling unitsSteven G. Bratsch Cite this: J. Chem. Educ. 1985, 62, 2, 101Publication Date (Print):February 1, 1985Publication History Received3 August 2009Published online1 February 1985Published inissue 1 February 1985https://doi.org/10.1021/ed062p101RIGHTS & PERMISSIONSArticle Views1198Altmetric-Citations118LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alerts Get e-Alerts
Enhanced Production of α-Galactosyl Epitopes by Metabolically Engineered Pichia pastoris (2003)

A metabolically engineered Pichia pastoris strain was constructed that harbored three heterologous enzymes: an S11E mutated sucrose synthase from Vigna radiata, a truncated UDP-glucose C4 epimerase from Saccharomyces cerevisiae, and a truncated bovine alpha-1,3-galactosyltransferase. Each gene has its own methanol-inducible alcohol oxidase 1 promoter and transcription terminator on the chromosomal DNA of P. pastoris strain GS115. The proteins were coexpressed intracellularly under the induction of methanol. After permeabilization, the whole P. pastoris cells were used to synthesize alpha-galactosyl (alpha-Gal) trisaccharide (Galalpha1,3Galbeta1,4Glc) with in situ regeneration of UDP-galactose. Up to 28 mM alpha-Gal was accumulated in a 200-ml reaction. The Pichia system described here is simple and flexible. This work demonstrates that recombinant P. pastoris is an excellent alternative to Escherichia coli transformants in large-scale synthesis of oligosaccharides.
Comparative sequence studies of rat skin and tendon collagen. II. Absence of a short sequence at the amino terminus of the skin α1 chain (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComparative sequence studies of rat skin and tendon collagen. II. Absence of a short sequence at the amino terminus of the skin α1 chainPaul BornsteinCite this: Biochemistry 1969, 8, 1, 63–71Publication Date (Print):January 1, 1969Publication History Published online1 May 2002Published inissue 1 January 1969https://pubs.acs.org/doi/10.1021/bi00829a010https://doi.org/10.1021/bi00829a010research-articleACS PublicationsRequest reuse permissionsArticle Views50Altmetric-Citations57LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Polyphosphates and Other Phosphorus-Containing Polymers for Drug Delivery Applications (2003)

Poly(phosphate ester)s, polyphosphonates, and polyphosphazenes are three classes of phosphorus-containing polymers that have received wide attention over the past decade for their utility in biomedicine and tissue engineering. These three families of polymers can lead to a number of subclasses of polymers with varied properties. Significant research in this area has led to niche polymers with morphologies ranging from viscous gels to amorphous microparticles for utility in drug delivery. Furthermore, the pentavalency of phosphorus offers the potential for covalent linking of the drug. The classes of polymers discussed in this review are being explored in human clinical trials for vaccine delivery as well as delivery of oncolytic and CNS therapeutics. More applications in the areas of DNA delivery and tissue engineering are also being explored.
Molecular cloning, sequencing, and expression of functional bovine herpesvirus 1 glycoprotein gIV in transfected bovine cells (1990)

The gene encoding bovine herpesvirus 1 (BHV-1) glycoprotein gIV was mapped, cloned, and sequenced. The gene is situated between map units 0.892 and 0.902 and encodes a predicted protein of 417 amino acids with a signal sequence cleavage site between amino acids 18 and 19. Comparison of the BHV-1 amino acid sequence with the homologous glycoproteins of other alphaherpesviruses, including herpes simplex virus type 1 glycoprotein gD, revealed significant homology in the amino-terminal half of the molecules, including six invariant cysteine residues. The identity of the open reading frame was verified by expression of the authentic recombinant BHV-1 gIV in bovine cells by using eucaryotic expression vectors pRSDneo (strong, constitutive promoter) and pMSG (weak, dexamethasone-inducible promoter). Constitutive expression of gIV proved toxic to cells, since stable cell lines could only be established when the gIV gene was placed under the control of an inducible promoter. Expression of gIV was cell associated and localized predominantly in the perinuclear region, although nuclear and plasma membrane staining was also observed. Radioimmunoprecipitation revealed that the recombinant glycoprotein was efficiently processed and had a molecular weight similar to that of the native form of gIV expressed in BHV-1-infected bovine cells. Recombinant gIV produced in the transfected bovine cells induced cell fusion, polykaryon formation, and nuclear fusion. In addition, expression of gIV interfered with BHV-1 replication in the transfected bovine cells.
Narodowy plan rozwoju jako przesłanka planowania finansowego w zakresie ochrony środowiska (2005)

"W sferze prowadzenia publicznej działalności finansowej istotnym przejawem&#13;\npraktycznej realizacji zasady racjonalnego gospodarowania jest odpowiednie&#13;\nuporządkowanie celów polityki finansowej państwa1. Wraz z przystąpieniem&#13;\nPolski do Unii Europejskiej podstawowym instrumentem określania&#13;\ncelów polityki finansowej państwa stało się planowanie. Proces harmonizacji&#13;\nprawa polskiego z prawem Unii Europejskiej doprowadził do tworzenia&#13;\nlicznych planów i strategii dalszego rozwoju właściwie we wszystkich dziedzinach&#13;\nzwiązanych z funkcjonowaniem państwa. Polityka planowania pozostaje&#13;\nw bezpośrednim związku z możliwością pozyskiwania przez Polskę&#13;\nśrodków funduszy strukturalnych."(...)
Potential of Murine IgG1 and Human IgG4 to Inhibit the Classical Complement and Fcγ Receptor Activation Pathways (2018)

IgG antibodies (Abs) mediate their effector functions through the interaction with Fcγ receptors (FcγRs) and the complement factors. The main IgG-mediated complement activation pathway is induced through the binding of complement C1q to IgG Abs. This interaction is dependent on antigen-dependent hexamer formation of human IgG1 and IgG3 to increase the affinity for the six-headed C1q molecule. By contrast, human IgG4 fails to bind to C1q. Instead, it has been suggested that human IgG4 can block IgG1 and IgG3 hexamerization required for their binding to C1q and activating the complement. Here, we show that murine IgG1, which functionally resembles human IgG4 by not interacting with C1q, inhibits the binding of IgG2a, IgG2b, and IgG3 to C1q <i>in vitro</i>, and suppresses IgG2a-mediated complement activation in a hemolytic assay in an antigen-dependent and IgG subclass-specific manner. From this perspective, we discuss the potential of murine IgG1 and human IgG4 to block the complement activation as well as suppressive effects of sialylated IgG subclass Abs on FcγR-mediated immune cell activation. Accumulating evidence suggests that both mechanisms seem to be responsible for preventing uncontrolled IgG (auto)Ab-induced inflammation in mice and humans. Distinct IgG subclass distributions and functionally opposite IgG Fc glycosylation patterns might explain different outcomes of IgG-mediated immune responses and provide new therapeutic options through the induction, enrichment, or application of antigen-specific sialylated human IgG4 to prevent complement and FcγR activation as well.
Use of polymers as protecting groups in organic synthesis. III. Selective functionalization of polyhydroxy alcohols (1976)

Insoluble polymers containing trityl chloride residues were used to block one primary alcohol functional group of several polyhydroxy alcohols. After protecting the remaining hydroxyl groups by benzoylation, the ether linkage between the polymer and the protected alcohol was cleaved in acidic medium. Depending on the reaction conditions and the nature of the starting alcohol, several alcohols or bromides in which only one of the two primary hydroxyls had been esterified, were obtained. In some cases benzoyl migrations were observed. The trityl chloride polymers could be regenerated in one step without degradation or appreciable loss of activity.
A revised classification of subtribe Helianthinae (Asteraceae: Heliantheae). I. Basal lineages (2002)

Results for nuclear ITS sequences were combined with previously obtained data from cpDNA restriction site studies to provide a comprehensive molecular phylogeny for subtribe Helianthinae. Results from the two molecular data sets were mostly congruent for basally diverging branches of the subtribe. Based on the results, combined with morphological observations, the basally diverging branches are placed in six genera, including one newly described. Bahiopsis is resurrected to accommodate species previously placed in Viguiera subgenus Bahiopsis. Calanticaria is newly proposed for the five species of Viguiera ser. Brevifolieae. Heliomeris is retained for the group sometimes included within Viguiera as sect. Heliomeris. Hymenostephium is revived and enlarged to include a number of species of similar habit and involucre, including members of Viguiera sect. Diplostichis, Haplocalymma and Garcilassa. Although cpDNA results place the morphologically distinctive Sclerocarpus within the clade including species of Hymenostephium, the ITS data were consistent with morphology in suggesting it to be a distinctive lineage.
A 32 kbit Variable Length Shift Register for Digital Audio Application (1986)

On this chip dynamic shift registers are combined with high density CCD SPS memory blocks. The shift register length can be adjusted from 17 to 32767 clock periods. The chip has been realized in a 2.5 μn NMCS process with CCD option.
Axonal guidance by an avoidance mechanism. (1990)

1. On a substrate consisting of alternating lanes of anterior and posterior tectal membranes, temporal retinal axons have a strong tendency to grow on the lanes of anterior membranes and to avoid the lanes of posterior membranes. 2. Temporal axons do extend neurites on posterior material, and in equivalent numbers and lengths to that of anterior membranes if the substrate consists of pure anterior or posterior membranes. 3. Inactivation of posterior membranes by heat or the enzyme phosphatidylinositol-specific phospholipase C (PI-PLC) abolishes their ability to induce the avoidance reaction of temporal axons. It is concluded that the posterior membranes contain a repulsive component for temporal retinal axons. 4. Growth cones growing on anterior membranes, which encounter posterior membranes at the strip boundary, in general do not become reduced in their growth rate. 5. These results are most easily explained by a "gradient-reading model" similar to chemotaxis where the steering of a growth cone is independent on the growth rate. 6. According to the model, a gradient of a guiding component outside the growth cone is transformed into an internal gradient which gives the growth cone its directionality. 7. Other models like growth inhibition cannot be ruled out but need at least two additional assumptions like habituation for growth on the putative posterior inhibitory substrate and a strong local restriction of the inhibitory effect within the growth cone which contacts the posterior material.
Incremental compilation in Magpie (1984)

Magpie is an interactive, integrate programming environment that supports the development of Pascal programs a powerful, single-user workstation. Incremental compilation techniques are used to provide quick feedback on static errors and to ensure that a program is always ready for execution even while it is being edited. Magpie performs syntax and static semantic analysis with the unit of incrementality of a single character. Magpie uses incremental compilation to achieve performance levels that allow Pascal to be used interactively and as its own debugging language.
Treatment patterns and health outcomes in metastatic renal cell carcinoma patients treated with targeted systemic therapies in the UK (2020)

In this study, treatment was consistent with current National Institute for Health and Care Excellence (NICE) guidelines for mRCC patients. Although the study population favoured poorer prognosis patients, outcomes were more favourable than those for England at the same time. However, overall survival in this 'real-world' population remains poor and indicates significant unmet need for effective and safe treatment options to improve survival among mRCC patients.
flp gene disruption in a parasitic nematode reveals motor dysfunction and unusual neuronal sensitivity to RNA interference (2007)

The potato cyst nematode Globodera pallida is a serious pest of potato crops. Nematode FMRFamide-like peptides (FLPs) are one of the most diverse neuropeptide families known, and modulate sensory and motor functions. As neuromuscular function is a well-established target for parasite control, parasitic nematode FLP signaling has significant potential in novel control strategies. In the absence of transgenic parasitic nematodes and the reported ineffectiveness of neuronal gene RNAi in Caenorhabditis elegans, nothing is known about flp function in nematode parasites. In attempts to evaluate flp function in G. pallida, we have discovered that, unlike in C. elegans, these genes are readily susceptible to RNAi. Silencing any of the five characterized G. pallida flp genes (Gp-flp-1, -6, -12, -14, or -18) incurred distinct aberrant behavioral phenotypes consistent with key roles in motor function. Further delineation of these effects revealed that double-stranded RNA exposure time (> or = 18 h) and concentration (> or = 0.1 microg/ml) were critical to the observed effects, which were reversible. G. pallida flp genes are essential to coordinated locomotory activities, do not display redundancy, and are susceptible to RNAi, paving the way for the investigation of RNAi-mediated flp gene silencing as a novel plant parasite control strategy.
Gene Expression Profiling: From Microarrays to Medicine (2004)

With the mapping of the human genome comes the ability to identify genes of interest in specific diseases and the pathways involved therein. Laboratory technology has evolved in parallel, providing us with the ability to assay thousands of these genes at once, a technique known as microarray analysis. The main question that this type of technology raises is how we can apply this powerful technology to clinical medicine. Recently, advances in data analysis, as well as standardization of the technology, have allowed us to examine this question, and indeed a few clinical trials currently being performed include microarrays as part of their protocol. In this review we outline the microarray technique and describe these types of studies in further detail.
Evidence of transmission ofBurkholderia cepacia,Burkholderia multivoransandBurkholderia dolosaamong persons with cystic fibrosis (2003)

Previous studies have identified specific Burkholderia cepacia complex strains that are common to multiple persons with cystic fibrosis (CF). Such so-called epidemic strains have an apparent enhanced capacity for inter-patient spread and reside primarily in Burkholderia cenocepacia (formerly B. cepacia complex genomovar III). We sought to identify strains from B. cepacia complex species other than B. cenocepacia that are similarly shared by multiple CF patients. We performed genotype analysis of 360 recent sputum culture isolates from 360 persons residing in 29 cities by using repetitive extragenic palendromic polymerase chain reaction (rep-PCR) and pulsed field gel electrophoresis. The results indicate that sharing of a common Burkholderia multivorans strain occurs relatively infrequently; however, several small clusters of patients infected with the same strain were identified. A cluster of seven patients infected with the same B. cepacia (genomovar I) strain was found. We also identified a large group of 28 patients receiving care in the same treatment center and infected with the same Burkholderia dolosa strain. These observations suggest that B. cepacia complex strains in species other than B. cenocepacia may be spread among CF patients.
The use of thrombin inhibitors and aprotinin in the preservation of platelets stored for transfusion. (1989)

We have evaluated the use of several thrombin inhibitors (heparin, Fragmin, hirudin, and Thromstop) in combination with cyclic adenosine 3',5'-monophosphate-active agents (prostaglandin E-1 [PGE-1] plus theophylline) and aprotinin for preparation and extended storage of platelet concentrates. Replacement of citrate with heparin resulted in accelerated clumping of platelets during storage. Fragmin, a low molecular weight heparin fraction, induced a high degree of platelet clumping, even in the presence of a standard amount of citrate (citrate-phosphate-dextrose-adenine formula 1 [CPDA-1]) plus PGE-1 and theophylline. The best anticoagulant formulation appeared to be CPDA-1 containing PGE-1 plus theophylline plus aprotinin, plus either hirudin or Thromstop for preservation of platelet responsiveness and structural integrity by in vitro markers. In eight platelet concentrates prepared with these inhibitors and stored for 15 days, the plasma pH was 6.50 +/- 0.15, the PO2 was 94 +/- 29 mm Hg, the PCO2 was 27 +/- 4 mm Hg, and the hypotonic shock response was 76% +/- 25% of the initial value recorded at Day 1 (all values expressed as mean +/- SD). Only 11% +/- 3% of the cellular lactate dehydrogenase was released, 69% +/- 8% of the platelets appeared to be in a discoid shape, and the response to 20 mumol/L adenosine 5'-diphosphate remained at 50% +/- 17% of the initial value. These results were all significantly different (p less than 0.01) from data obtained for concentrates prepared without aprotinin. The use of a factor Xa inhibitor (diamidino-benzofuranyl ethane) in place of Thromstop or hirudin did not provide substantial improvement over controls without inhibitors.
Homodimerization Controls the Fibroblast Growth Factor 9 Subfamily's Receptor Binding and Heparan Sulfate-Dependent Diffusion in the Extracellular Matrix (2009)

Uncontrolled fibroblast growth factor (FGF) signaling can lead to human diseases, necessitating multiple layers of self-regulatory control mechanisms to keep its activity in check. Herein, we demonstrate that FGF9 and FGF20 ligands undergo a reversible homodimerization, occluding their key receptor binding sites. To test the role of dimerization in ligand autoinhibition, we introduced structure-based mutations into the dimer interfaces of FGF9 and FGF20. The mutations weakened the ability of the ligands to dimerize, effectively increasing the concentrations of monomeric ligands capable of binding and activating their cognate FGF receptor in vitro and in living cells. Interestingly, the monomeric ligands exhibit reduced heparin binding, resulting in their increased radii of heparan sulfate-dependent diffusion and biologic action, as evidenced by the wider dilation area of ex vivo lung cultures in response to implanted mutant FGF9-loaded beads. Hence, our data demonstrate that homodimerization autoregulates FGF9 and FGF20's receptor binding and concentration gradients in the extracellular matrix. Our study is the first to implicate ligand dimerization as an autoregulatory mechanism for growth factor bioactivity and sets the stage for engineering modified FGF9 subfamily ligands, with desired activity for use in both basic and translational research.
Machine organization of the IBM RISC System/6000 processor (1990)

The IBM RISC System/6000* processor is a second-generation RISC processor which reduces the execution pipeline penalties caused by branch instructions and also provides high floating-point performance. It employs multiple functional units which operate concurrently to maximize the instruction execution rate. By employing these advanced machine-organization techniques, it can execute up to four instructions simultaneously. Approximately 11 MFLOPS are achieved on the LINPACK benchmarks.
Two-handed input in a compound task (1994)

Article Two-handed input in a compound task Share on Authors: Paul Kabbash Computer Systems Research Institute, University of Toronto, Toronto, Ont., M5S 1A4, Canada Computer Systems Research Institute, University of Toronto, Toronto, Ont., M5S 1A4, CanadaView Profile , William Buxton Computer Systems Research Institute, University of Toronto, Toronto, Ont., M5S 1A4, Canada and Xerox Palo Alto Research Center, 3333 Coyote Hill Rd., Palo Alto, CA Computer Systems Research Institute, University of Toronto, Toronto, Ont., M5S 1A4, Canada and Xerox Palo Alto Research Center, 3333 Coyote Hill Rd., Palo Alto, CAView Profile , Abigail Sellen Rank Xerox EuroPARC, 61 Regent St., Cambridge, CB2 1AB, U.K and MRC Applied Psychology Unit, 15 Chaucer Rd., Cambridge, CB2 2EF, U.K. Rank Xerox EuroPARC, 61 Regent St., Cambridge, CB2 1AB, U.K and MRC Applied Psychology Unit, 15 Chaucer Rd., Cambridge, CB2 2EF, U.K.View Profile Authors Info & Claims CHI '94: Proceedings of the SIGCHI Conference on Human Factors in Computing SystemsApril 1994 Pages 417–423https://doi.org/10.1145/191666.191808Online:24 April 1994Publication History 106citation1,523DownloadsMetricsTotal Citations106Total Downloads1,523Last 12 Months42Last 6 weeks3 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access