gebhart/patrepeval huggingface dataset:
Small Bandgap Polymers for Organic Solar Cells(Polymer Material Development in the Last 5 Years) (2008)

During the last decade the field of polymer photovoltaics has seen a tremendous improvement in both device efficiency and understanding of the underlying physical processes. One has come to a point in which the prototypical large bandgap material system P3HT:PCBM is nearing optimal device performance. In order to enhance efficiencies even further, research activities for new materials are needed with better aligned energy levels. One interesting approach is by narrowing the donor bandgap to enhance light absorption. Recent developments on small band gap (<2.0 eV) materials for photovoltaic applications are reviewed. First, an introduction is given regarding the processes governing the exciton dissociation, charge transport requirements, energy level engineering of both donor and acceptor materials, and other parameters determining the photovoltaic performance. The focus is on polymeric donor materials, which are subdivided by the type of monomeric units that constitute the backbone. Finally, the synthetic methods and conditions, processing of the devices, and the device performances are summarized.
[Protein expression, purification of PCGF1 and its monoclonal antibody preparation and identification]. (2011)

To express the human recombinant PCGF1 protein and prepare monoclonal antibody (mAb) against it.The recombinant expression plasmid pET32a-His-PCGF1-128/189 was made and transformed into E.coli (BL21), and then the recombinant fusion protein His-PCGF1-128/189 was expressed and purified. The BALB/c mice were immuned with purified protein His-PCGF1-128/189 as antigen. The mAbs against PCGF1 were prepared by using standard hybridoma technique. The hybridoma cell lines were obtained by ELISA and Western blot screening procedure, the isotype of the mAbs were further identified by immune-double diffusion. Ascites were prepared from one propagated hybridoma cell line and mAbs were purified by using the Kit from Millipore. The valence of mAb was detected by Western blot.The recombinant protein His-PCGF1-128/189 was expressed and purified. Two hybfidmas producing antibodies against PCGF1 were obtained, the isotypes of two mAbs were IgG1, Western blot showed that the antibodies were high sensitive(1:6 000) and high specific for PCGF1.The anti-PCGF1 mAb prepared by using recombinant His-PCGF1-128/189 protein as antigen can be used for detecting PCGF1 proteins which are either endogenous or exogenous.
Sacral Nerve Stimulation for Treatment of Intractable Pain Associated with Cauda Equina Syndrome (2010)

Sacral nerve stimulation (SNS) is an effective treatment for bladder and bowel dysfunction, and also has a role in the treatment of chronic pelvic pain. We report two cases of intractable pain associated with cauda equina syndrome (CES) that were treated successfully by SNS. The first patient suffered from intractable pelvic pain with urinary incontinence and fecal incontinence after surgery for a herniated lumbar disc. The second patient underwent surgery for treatment of a burst fracture and developed intractable pelvic area pain, right leg pain, excessive urinary frequency, urinary incontinence, voiding difficulty and constipation one year after surgery. A SNS trial was performed on both patients. Both patients' pain was significantly improved and urinary symptoms were much relieved. Neuromodulation of the sacral nerves is an effective treatment for idiopathic urinary frequency, urgency, and urge incontinence. Sacral neuromodulation has also been used to control various forms of pelvic pain. Although the mechanism of action of neuromodulation remains unexplained, numerous clinical success reports suggest that it is a therapy with efficacy and durability. From the results of our research, we believe that SNS can be a safe and effective option for the treatment of intractable pelvic pain with incomplete CES.
Experiments on Point-to-Point Position Control of a Flexible Beam Using Laplace Transform Technique: Part I—Open-Loop (1991)

When lightly damped flexible structures are used in high bandwidth applications, the elimination of residual vibration during point-to-point positioning is an important engineering problem. Using the Laplace domain synthesis technique introduced in earlier publications, experiments on the precise point-to-point position control of a flexible beam have been performed. In Part I of this two-part paper, results related to open-loop control are presented. A variety of candidate control functions are evaluated and performance issues related to robustness and sensitivity are investigated.
Analysis of gene expression profiles in normal and neoplastic ovarian tissue samples identifies candidate molecular markers of epithelial ovarian cancer (2001)

Epithelial ovarian cancer is the leading cause of death from gynecologic cancer, in part because of the lack of effective early detection methods. Although alterations of several genes, such as c-erb-B2, c-myc, and p53, have been identified in a significant fraction of ovarian cancers, none of these mutations are diagnostic of malignancy or predictive of tumor behavior over time. Here, we used oligonucleotide microarrays with probe sets complementary to >6,000 human genes to identify genes whose expression correlated with epithelial ovarian cancer. We extended current microarray technology by simultaneously hybridizing ovarian RNA samples in a highly parallel manner to a single glass wafer containing 49 individual oligonucleotide arrays separated by gaskets within a custom-built chamber (termed "array-of-arrays"). Hierarchical clustering of the expression data revealed distinct groups of samples. Normal tissues were readily distinguished from tumor tissues, and tumors could be further subdivided into major groupings that correlated both to histological and clinical observations, as well as cell type-specific gene expression. A metric was devised to identify genes whose expression could be considered ideal for molecular determination of epithelial ovarian malignancies. The list of genes generated by this method was highly enriched for known markers of several epithelial malignancies, including ovarian cancer. This study demonstrates the rapidity with which large amounts of expression data can be generated. The results highlight important molecular features of human ovarian cancer and identify new genes as candidate molecular markers.
Phase I trial of subcutaneous recombinant human interleukin‐2 in patients with metastatic melanoma (2002)

SC IL-2 was well tolerated and had high sustained bioavailability at the higher doses studied. The MTD for a daily SC regimen was 12 MIU/m(2) and is recommended for future studies.
Tissue‐specific expression of ATCYS‐3A, a gene encoding the cytosolic isoform of O‐acetylserine(thiol)lyase in Arabidopsis (1997)

Atcys-3A from Arabidopsis encodes the cytosolic isoform of O-acetylserine(thiol)lyase that catalyzes the last step of cysteine biosynthesis. The Atcys-3A transcript is present in different organs of mature plants, being more abundant in roots and declining to 40-50% in rosette leaves and flowers. In situ hybridization studies have shown a high Atcys-3A signal in root tissues, mainly localized to the cortex and xylem parenchyma. In a flower before anthesis, the transcript is detected exclusively in anthers and sepals and evenly distributed throughout the receptacle of the flower. An unexpected observation from these studies is the highest expression of Atcys-3A mRNA found in trichomes of either leaf or stem. The presence of high levels of the transcript is observed very early in trichome cell development. This is the first report describing the cellular localization of any plant O-acetylserine(thiol)lyase mRNA. The high level of Atcys-3A expression in trichomes raises new aspects to the biological function of trichomes, related to sulfate metabolism.
Japanese phase I study ofGC33, a humanized antibody against glypican‐3 for advanced hepatocellular carcinoma (2014)

GC33 is a humanized mAb against human glypican-3 (GPC3). In the first-in-human study carried out in the USA, GC33 was well tolerated and showed preliminary antitumor activity in patients with advanced hepatocellular carcinoma. This study aimed to assess the safety, tolerability, and pharmacokinetic characteristics of GC33 in Japanese patients with advanced hepatocellular carcinoma. The study design was a conventional 3 + 3 dose-escalation design to determine the maximum tolerated dose of GC33 given i.v. at 5, 10, or 20 mg/kg weekly. Immunohistochemistry was carried out on tumor biopsies to evaluate GPC3 expression. Thirteen patients were enrolled across the three dose levels, and no patients observed any dose-limiting toxicity up to the highest planned dose of 20 mg/kg. The most common adverse events were decreased lymphocyte count, decreased natural killer cell count, increased C-reactive protein, and pyrexia. Grade 3 adverse events (increased blood pressure, decreased lymphocyte count, and decreased platelet count) were observed in two or more patients. The AUCinf showed a dose-proportional increase from the 5 mg/kg dose group to the 20 mg/kg dose group. The trough concentrations of GC33 appeared to reach a steady state after the fourth to the sixth dose. Seven of the 13 patients showed stable disease, the other six showed progressive disease. Furthermore, three patients showed long-term stable disease of more than 5 months. In conclusion, GC33 given at up to 20 mg/kg weekly was well tolerated in Japanese patients with advanced hepatocellular carcinoma.
Neural Stimulation and Recording Electrodes (2008)

Electrical stimulation of nerve tissue and recording of neural electrical activity are the basis of emerging prostheses and treatments for spinal cord injury, stroke, sensory deficits, and neurological disorders. An understanding of the electrochemical mechanisms underlying the behavior of neural stimulation and recording electrodes is important for the development of chronically implanted devices, particularly those employing large numbers of microelectrodes. For stimulation, materials that support charge injection by capacitive and faradaic mechanisms are available. These include titanium nitride, platinum, and iridium oxide, each with certain advantages and limitations. The use of charge-balanced waveforms and maximum electrochemical potential excursions as criteria for reversible charge injection with these electrode materials are described and critiqued. Techniques for characterizing electrochemical properties relevant to stimulation and recording are described with examples of differences in the in vitro and in vivo response of electrodes.
Incidence and nature of CD20‐negative relapses following rituximab therapy in aggressive B‐cell non‐Hodgkin's lymphoma: a retrospective review (2002)

Summary. Re‐treatment with rituximab for B‐cell non‐Hodgkin's lymphoma (NHL) relapsing after previous rituximab therapy has recently been shown to be clinically efficacious. Although the mechanism of resistance to rituximab re‐treatment in non‐responding patients is unknown, it is possible that loss of CD20 expression in the relapsed NHL could be important in some patients. We examined the incidence and nature of CD20 negative relapses following rituximab therapy in aggressive B‐cell NHL treated at our institution. Of a total of 18 patients who received rituximab, 13 have relapsed, with 10 patients subsequently undergoing repeat tissue biopsy. Six of these 10 patients (60%) were shown to have lost CD20 expression by either immunohistochemistry and/or flow cytometry. Furthermore, three of the six patients who relapsed with CD20‐negative NHL also suffered relapses at unusual anatomical sites. We conclude that loss of CD20 expression in aggressive B‐cell NHL relapsing post‐rituximab therapy is common. As such, repeat tissue biopsy should be undertaken to document CD20 expression by both flow cytometry and immunohistochemistry prior to considering repeated courses of rituximab in relapsed aggressive lymphomas.
Cre/lox-regulated transgenic zebrafish model with conditional myc-induced T cell acute lymphoblastic leukemia (2005)

We have created a stable transgenic rag2-EGFP-mMyc zebrafish line that develops GFP-labeled T cell acute lymphoblastic leukemia (T-ALL), allowing visualization of the onset and spread of this disease. Here, we show that leukemias from this transgenic line are highly penetrant and render animals moribund by 80.7 +/- 17.6 days of life (+/-1 SD, range = 50-158 days). These T cell leukemias are clonally aneuploid, can be transplanted into irradiated recipient fish, and express the zebrafish orthologues of the human T-ALL oncogenes tal1/scl and lmo2, thus providing an animal model for the most prevalent molecular subgroup of human T-ALL. Because T-ALL develops very rapidly in rag2-EGFP-mMyc transgenic fish (in which "mMyc" represents mouse c-Myc), this line can only be maintained by in vitro fertilization. Thus, we have created a conditional transgene in which the EGFP-mMyc oncogene is preceded by a loxed dsRED2 gene and have generated stable rag2-loxP-dsRED2-loxP-EGFP-mMyc transgenic zebrafish lines, which have red fluorescent thymocytes and do not develop leukemia. Transgenic progeny from one of these lines can be induced to develop T-ALL by injecting Cre RNA into one-cell-stage embryos, demonstrating the utility of the Cre/lox system in the zebrafish and providing an essential step in preparing this model for chemical and genetic screens designed to identify modifiers of Myc-induced T-ALL.
Bacterial Persister Cell Formation and Dormancy (2013)

Bacterial cells may escape the effects of antibiotics without undergoing genetic change; these cells are known as persisters. Unlike resistant cells that grow in the presence of antibiotics, persister cells do not grow in the presence of antibiotics. These persister cells are a small fraction of exponentially growing cells (due to carryover from the inoculum) but become a significant fraction in the stationary phase and in biofilms (up to 1%). Critically, persister cells may be a major cause of chronic infections. The mechanism of persister cell formation is not well understood, and even the metabolic state of these cells is debated. Here, we review studies relevant to the formation of persister cells and their metabolic state and conclude that the best model for persister cells is still dormancy, with the latest mechanistic studies shedding light on how cells reach this dormant state.
E6 of human papillomavirus type 16 can overcome the M1 stage of immortalization in human mammary epithelial cells but not in human fibroblasts. (1993)

Immortalization is the consequence of the inactivation or bypass of two mortality stage mechanisms, M1 and M2, which are controlled by several genes including Rb and p53 in human fibroblasts. Abrogation of the M1 controls can be obtained through the activity of DNA tumor virus genes such as E6 and E7 of human papillomavirus 16 (HPV16). Fibroblasts expressing both E6 (which binds p53) and E7 (which binds Rb) bypass M1 and continue replicating (exhibit an extended lifespan) until an independent mechanism, M2, is activated. The inactivation of the M2 mechanism finally results in cell immortalization. The present study establishes a difference in the tissue-specific mechanisms for the control of the M1 stage of cellular senescence. The expression of HPV16 E6 was sufficient to bypass the M1 stage of cellular senescence and confer an extended lifespan in human mammary epithelial cells but not in fibroblasts. This implies that the M1 mechanism in human mammary epithelial cells does not involve the constitutive activation of Rb function as it does in fibroblasts. In addition, the results confirmed that the expression of HPV16 E6 (or both E6 and E7) did not directly immortalize the human mammary epithelial cells, since the inactivation of a second event, M2, was required to achieve immortalization. These observations are considered in the context of the telomere shortening model of cellular senescence.
Capillary array electrophoresis using laser-excited confocal fluorescence detection (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCapillary array electrophoresis using laser-excited confocal fluorescence detectionXiaohua C. Huang, Mark A. Quesada, and Richard A. MathiesCite this: Anal. Chem. 1992, 64, 8, 967–972Publication Date (Print):April 15, 1992Publication History Published online1 May 2002Published inissue 15 April 1992https://pubs.acs.org/doi/10.1021/ac00032a025https://doi.org/10.1021/ac00032a025research-articleACS PublicationsRequest reuse permissionsArticle Views345Altmetric-Citations163LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Norma cluster (ACO3627) - II. The near-infrared -band luminosity function (2009)

A deep K_s-band photometric catalogue of galaxies at the core of the rich, nearby Norma cluster (ACO3627) is presented. The survey covers about 45 by 45 square arcmin (slightly less than 1/3 Abell radius), which corresponds to approx. 0.8 Mpc^2 at the adopted distance (v_cmb/H0) of 70 Mpc of this cluster. The survey is estimated to be complete to a magnitude of M_Ks <~ -19.5 mag. This extends into the dwarf regime, 6 magnitudes below M_Ks*. The catalogue contains 390 objects, 235 of which are classified as likely or definite galaxies and 155 as candidate galaxies. The Ks-band luminosity function (LF) is constructed from the photometric sample, using a spectroscopic subsample to correct for fore- and background contamination. We fit a Schechter function with a characteristic magnitude of M_Ks* = -25.39 \pm 0.80 mag and faint-end slope of alpha = -1.26 \pm 0.10 to the data. The shape of the LF is similar to those found in previous determinations of the cluster LF, in both optical and near infrared. The Schechter parameters agree well with those of recent field LFs, suggesting that both the shape of the bright end and the faint end slope are relatively insensitive to environment.
Photoinduced phenomena in lead magnesium niobate crystal at the diffuse phase transition (1978)

An investigation of the photoinduced change of the electrooptic effect in lead magnesium niobate crystal has been earned out. The increase of the half-wave voltage has been measured when the crystal is illuminated with photoactive light. A change of frequency dependence of the electrooptic coefficient measured in a strong alternating electric field has been observed under illumination. The trap levels have been evaluated using the results of the conductivity and thermostimulated current investigations. The optical transmission and photoconductivity spectra of the reduced PMN crystal have been measured. The nature of the photoinduced phenomena in diffuse phase transition ferroelectrics has been discussed.
Pro-oxidant and cytotoxic effects of circulating heme (2002)

Numerous pathologies may involve toxic side effects of free heme and heme-derived iron. Deficiency of the heme-catabolizing enzyme, heme oxygenase-1 (HO-1), in both a human patient and transgenic knockout mice leads to an abundance of circulating heme and damage to vascular endothelium. Although heme can be directly cytotoxic, the present investigations examine the possibility that hemoglobin-derived heme and iron might be indirectly toxic through the generation of oxidized forms of low-density lipoprotein (LDL). In support, hemoglobin in plasma, when oxidized to methemoglobin by oxidants such as leukocyte-derived reactive oxygen, causes oxidative modification of LDL. Heme, released from methemoglobin, catalyzes the oxidation of LDL, which in turn induces endothelial cytolysis primarily caused by lipid hydroperoxides. Exposure of endothelium to sublethal concentrations of this oxidized LDL leads to induction of both HO-1 and ferritin. Similar endothelial cytotoxicity was caused by LDL isolated from plasma of an HO-1-deficient child. Spectral analysis of the child's plasma revealed a substantial oxidation of plasma hemoglobin to methemoglobin. Iron accumulated in the HO-1-deficient child's LDL and several independent assays revealed oxidative modification of the LDL. We conclude that hemoglobin, when oxidized in plasma, can be indirectly cytotoxic through the generation of oxidized LDL by released heme and that, in response, the intracellular defense-HO-1 and ferritin-is induced. These results may be relevant to a variety of disorders-such as renal failure associated with intravascular hemolysis, hemorrhagic injury to the central nervous system, and, perhaps, atherogenesis-in which hemoglobin-derived heme may promote the formation of fatty acid hydroperoxides.
Demonstration of large-angle nonmechanical laser beam steering based on LC polymer polarization gratings (2011)

Polarization gratings (PGs) as polarization sensitive diffractive optical elements work in broadband (UV to Mid- IR) with nearly 100% diffraction efficiency. We have introduced and utilized the PGs in different types of beam steering modules presented in our previous papers. Here, we describe and demonstrate a nonmechanical beam steering device based on passive gratings, liquid crystal (LC) polymer PGs. The device covers a large-angle Field-Of-Regard (FOR) with high efficiency, and is based on a stack of alternating LC half-wave plates and LC polymer PGs. The half-wave plates are switchable and are used to select the handedness of the circularly polarized input beam. The polymer PGs diffract the input beam to either of the first diffraction orders based on the circular handedness of the beam previously selected. When compared with conventional beam steering methods based on active gratings (ternary and quasi-ternary designs), this technique is experimentally able to steer an equivalent number of angles with similar efficiency, but fewer LC cells, and hence, fewer transparent electrodes and lower absorption. We successfully demonstrate the ability to steer 80&deg; FOR with roughly 2.6&deg; resolution at 1064 nm wavelength.
MicroRNA-Mediated In Vitro and In Vivo Direct Reprogramming of Cardiac Fibroblasts to Cardiomyocytes (2012)

The findings from this study provide proof-of-concept that miRNAs have the capability of directly converting fibroblasts to a cardiomyocyte-like phenotype in vitro. Also of significance is that this is the first report of direct cardiac reprogramming in vivo. Our approach may have broad and important implications for therapeutic tissue regeneration in general.
Asymmetric Deprotonations:  Lithiation of N-(tert-Butoxycarbonyl)indoline with sec-Butyllithium/(−)-Sparteine (1997)

The asymmetric lithiation of N-Boc indoline (1) with s-BuLi/(−)-sparteine and subsequent substitution provides the 2-substituted N-Boc indolines 3 and 5−11 with excellent enantiomeric ratios and in variable yields. The asymmetric lithiation−substitution sequence with N-Boc-7-chloroindoline (12) provides products 13−19 with good enantiomeric ratios. Mechanistic investigation establishes that the enantioselectivities arise from an initial asymmetric deprotonation to provide the enantioenriched and configurationally stable organolithium intermediates (S)-28 and (S)-29, which react stereoselectively with electrophiles.