gebhart/patrepeval huggingface dataset:
Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. (1995)

Human interferon-inducible protein 10 (IP-10), a member of the alpha chemokine family, inhibits bone marrow colony formation, has antitumor activity in vivo, is chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells. Here we report that IP-10 is a potent inhibitor of angiogenesis in vivo. IP-10 profoundly inhibited basic fibroblast growth factor-induced neovascularization of Matrigel (prepared by H. K. Kleinman) injected subcutaneously into athymic mice. In addition, IP-10, in a dose-dependent fashion, suppressed endothelial cell differentiation into tubular capillary structures in vitro. IP-10 had no effect on endothelial cell growth, attachment, and migration as assayed in vitro. These results document an important biological property of IP-10 and raise the possibility that IP-10 may participate in the regulation of angiogenesis during inflammation and tumorigenesis.
Boundary-Aware Instance Segmentation (2017)

We address the problem of instance-level semantic segmentation, which aims at jointly detecting, segmenting and classifying every individual object in an image. In this context, existing methods typically propose candidate objects, usually as bounding boxes, and directly predict a binary mask within each such proposal. As a consequence, they cannot recover from errors in the object candidate generation process, such as too small or shifted boxes. In this paper, we introduce a novel object segment representation based on the distance transform of the object masks. We then design an object mask network (OMN) with a new residual-deconvolution architecture that infers such a representation and decodes it into the final binary object mask. This allows us to predict masks that go beyond the scope of the bounding boxes and are thus robust to inaccurate object candidates. We integrate our OMN into a Multitask Network Cascade framework, and learn the resulting boundary-aware instance segmentation (BAIS) network in an end-to-end manner. Our experiments on the PASCAL VOC 2012 and the Cityscapes datasets demonstrate the benefits of our approach, which outperforms the state-of-the-art in both object proposal generation and instance segmentation.
Multiple signalling pathways involved in β2‐adrenoceptor‐mediated glucose uptake in rat skeletal muscle cells (2006)

1. Beta-adrenoceptor (AR) agonists increase 2-deoxy-[3H]-D-glucose uptake (GU) via beta2-AR in rat L6 cells. The beta-AR agonists, zinterol (beta2-AR) and (-)-isoprenaline, increased cAMP accumulation in a concentration-dependent manner (pEC50=9.1+/-0.02 and 7.8+/-0.02). Cholera toxin (% max increase 141.8+/-2.5) and the cAMP analogues, 8-bromo-cAMP (8Br-cAMP) and dibutyryl cAMP (dbcAMP), also increased GU (196.8+/-13.5 and 196.4+/-17.3%). 2. The adenylate cyclase inhibitor, 2',5'-dideoxyadenosine (50 microM), significantly reduced cAMP accumulation to zinterol (100 nM) (109.7+35.0 to 21.6+4.5 pmol well(-1)), or forskolin (10 microM) (230.1+/-58.0 to 107.2+/-26.3 pmol well(-1)), and partially inhibited zinterol-stimulated GU (217+/-26.3 to 176.1+/-20.4%). The protein kinase A (PKA) inhibitor, 4-cyano-3-methylisoquinoline (100 nM), did not inhibit zinterol-stimulated GU. The PDE4 inhibitor, rolipram (10 microM), increased cAMP accumulation to zinterol or forskolin, and sensitised the GU response to zinterol, indicating a stimulatory role of cAMP in GU. 3. cAMP accumulation studies indicated that the beta2-AR was desensitised by prolonged stimulation with zinterol, but not forskolin, whereas GU responses to zinterol increased with time, suggesting that receptor desensitisation may be involved in GU. Receptor desensitisation was not reversed by inhibition of PKA or Gi. 4. PTX pretreatment (100 ng ml(-1)) inhibited insulin or zinterol-stimulated but not 8Br-cAMP or dbcAMP-stimulated GU. The PI3K inhibitor, LY294002 (1 microM), inhibited insulin- (174.9+/-5.9 to 142.7+/-2.7%) and zinterol- (166.9+/-7.6 to 141.1+/-8.1%) but not 8 Br-cAMP-stimulated GU. In contrast to insulin, zinterol did not cause phosphorylation of Akt. 5. The results suggest that GU in L6 cells involves three mechanisms: (1) an insulin-dependent pathway involving PI3K, (2) a beta2-AR-mediated pathway involving both cAMP and PI3K, and (3) a receptor-independent pathway suggested by cAMP analogues that increase GU independently of PI3K. PKA appears to negatively regulate beta2-AR-mediated GU.
Hedgehog Signaling Antagonist Promotes Regression of Both Liver Fibrosis and Hepatocellular Carcinoma in a Murine Model of Primary Liver Cancer (2011)

Hh pathway activation promotes liver fibrosis and hepatocarcinogenesis, and inhibiting Hh signaling safely reverses both processes even when fibrosis and HCC are advanced.
Velocity-matched distributed photodetectors with high-saturation power and large bandwidth (1996)

A high-power, high-bandwidth photodetector is experimentally demonstrated using a novel velocity-matched distributed photodetector (VMDP). The distributed photodetector structure can achieve large absorption volume and high-saturation power while maintaining the high-speed performance of the fast photodiodes. The VMDP with 56 mA saturation photocurrent and an instrument-limited 3-dB bandwidth of 49 GHz is achieved. The results show that VMDP is ideal for high-performance microwave fiber-optic links and high-power optical-microwave applications.
Intrastriatal transplantation of cross-species fetal striatal cells reduces abnormal movements in a primate model of Huntington disease. (1992)

Huntington disease is a neurological movement disorder involving massive neuronal death in the caudate-putamen region of the brain. Neither preventive nor curative therapy exists for this disease. The implantation of cross-species striatal neural precursor cells into the lesioned striatum of nonhuman primates (baboons) reduced the abnormal movements seen in the disease model. These abnormal movements reappeared after immunological rejection of the implanted striatal cells and were not modified by transplantation with nonstriatal cells. These findings encourage further experimentation toward the use of cell sources other than human fetal cells in a potential clinical application to Huntington disease.
Hybrid FES orthosis incorporating closed loop control and sensory feedback (1988)

A hybrid functional electrical stimulation (FES) orthosis is described, comprising a rigid ankle-foot brace, a multi-channel FES stimulator with surface electrodes, body mounted sensors, a 'rule-based' controller and an electro-cutaneous display for supplementary sensory feedback. The mechanical brace provides stability, without FES activation of muscles, for standing postures normally adopted by patients. This avoids inducing muscle fatigue during prolonged upright activity. However, stability is conditional upon the position of the ground reaction vector (GRV) relative to the knee joint. The finite state FES controller reacts automatically to destabilizing shifts of the GRV by stimulating appropriate anti-gravity musculature to brace the leg. The FES system also features a control mode to initiate and terminate flexion of the leg during forward progression. A simple mode of supplementary sensory feedback was used during the laboratory standing tests to assist the patient in maintaining a set posture. Preliminary results of laboratory tests for two spinal cord injured subjects are presented.
Two Lipoproteins Extracted from Escherichia coli K-12 LCD25 Lipopolysaccharide Are the Major Components Responsible for Toll-Like Receptor 2-Mediated Signaling (2002)

Toll-like receptor 2 (TLR2)-mediated cell activation induced by commercial preparations of LPS was recently shown to arise from impurities whose identities are not known. In this work, we determined the molecules responsible for TLR2-mediated cell activation in LPS derived from Escherichia coli K-12 strain LCD25. When LCD25 LPS was phenol extracted, two proteins capable of TLR2-mediated cell activation were purified and identified as E. coli lipoproteins. We cloned, expressed, and purified these two lipoproteins, Lip19 and Lip12. Lip19 or Lip12 activated TNF-alpha production from RAW264.7 and THP-1 cells in a TLR2-dependent manner. However, neither Lip19 nor Lip12 activated HUVECs, which lack endogenous TLR2. Additionally, IkappaB kinase beta and c-Jun N-terminal kinase 1 activation in THP-1 cells induced by Lip19 or Lip12 was observed. TLR2 activation by Lip19 and Lip12 in HEK293 cells was blocked by inhibitory anti-TLR2 mAbs. The unlipidated mutants, Lip19-C19S and Lip12-C21S, in which the NH(2)-terminal cysteine was substituted by serine, lost their ability to activate TLR2-transfected HEK 293 cells. Taken together, these results demonstrate that two lipoproteins constitute the major contaminants responsible for TLR2-mediated cell activation in E. coli LCD25 LPS.
High CD34+ Cell Counts Decrease Hematologic Toxicity of Autologous Peripheral Blood Progenitor Cell Transplantation (1998)

Optimal numbers of CD34(+) cells to be reinfused in patients undergoing peripheral blood progenitor cell (PBPC) transplantation after high-dose chemotherapy are still unknown. Hematologic reconstitution of 168 transplantations performed in patients with lymphoproliferative diseases was analyzed according to the number of CD34(+) cells reinfused. The number of days from PBPC reinfusion until neutrophil recovery (>1.0 x 10(9)/L) and unsustained platelet recovery (>50 x 10(9)/L) were analyzed in three groups defined by the number of CD34(+) cells reinfused: a low group with less than or equal to 2.5 x 10(6) CD34(+) cells/kg, a high group with greater than 15 x 10(6) CD34(+) cells/kg, and an intermediate group to which the former two groups were compared. The 22 low-group patients had a significantly delayed neutrophil (P < .0001) and platelet recovery (P < .0001). The 41 high-group patients experienced significantly shorter engraftment compared with the intermediate group with a median of 11 (range, 8 to 16) versus 12 (range, 7 to 17) days for neutrophil recovery (P = .003), and a median of 11 (range, 7 to 24) versus 14 (range, 8 to 180+) days for platelet recovery (P < .0001). These patients required significantly less platelet transfusions (P = .002). In a multivariate analysis, the amount of CD34(+) cells reinfused was the only variable showing significance for neutrophil and platelet recovery. High-group patients had a shorter hospital stay (P = .01) and tended to need fewer days of antibotic administration (P = .12). In conclusion, these results suggest that reinfusion of greater than 15 x 10(6) CD34(+) cells/kg after high-dose chemotherapy for lymphoproliferative diseases further shortens hematopoietic reconstitution, reduces platelet requirements, and may improve patients' quality of life.
The vibro-acoustic response and analysis of a full-scale aircraft fuselage section for interior noise reduction (2005)

The surface and interior response of a Cessna Citation fuselage section under three different forcing functions (10-1000 Hz) is evaluated through spatially dense scanning measurements. Spatial Fourier analysis reveals that a point force applied to the stiffener grid provides a rich wavenumber response over a broad frequency range. The surface motion data show global structural modes (approximately < 150 Hz), superposition of global and local intrapanel responses (approximately 150-450 Hz), and intrapanel motion alone (approximately > 450 Hz). Some evidence of Bloch wave motion is observed, revealing classical stop/pass bands associated with stiffener periodicity. The interior response (approximately < 150 Hz) is dominated by global structural modes that force the interior cavity. Local intrapanel responses (approximately > 150 Hz) of the fuselage provide a broadband volume velocity source that strongly excites a high density of interior modes. Mode coupling between the structural response and the interior modes appears to be negligible due to a lack of frequency proximity and mismatches in the spatial distribution. A high degree-of-freedom finite element model of the fuselage section was developed as a predictive tool. The calculated response is in good agreement with the experimental result, yielding a general model development methodology for accurate prediction of structures with moderate to high complexity.
Palladium-Mediated Macrocyclization on Solid Support and Its Applications to Combinatorial Synthesis (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPalladium-Mediated Macrocyclization on Solid Support and Its Applications to Combinatorial SynthesisMariann Hiroshige, James R. Hauske, and Peng ZhouCite this: J. Am. Chem. Soc. 1995, 117, 46, 11590–11591Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/ja00151a029https://doi.org/10.1021/ja00151a029research-articleACS PublicationsRequest reuse permissionsArticle Views289Altmetric-Citations62LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Toward the development of miniaturized imaging systems for detection of pre-cancer (2002)

In this paper, we describe the progress toward the development of miniaturized imaging systems with applications in medical imaging, and specifically, detection of pre-cancer. The focus of the article is a miniature, optical-sectioning, fluorescence microscope. The miniature microscope is constructed from lithographically printed optics and assembled using a bulk micro-machined silicon microoptical table. Optical elements have been printed in a negative tone hybrid glass to a maximum depth of 59 /spl mu/m and an rms surface roughness between 10-45 nm, fulfilling the requirements of the miniature microscope. Test optical elements have been assembled using silicon-spring equipped mounting slots. The design of silicon springs is presented in this paper. Optical elements can be assembled within the tolerances of an NA=0.4 miniature microscope objective, confirming the concept of simple, zero-alignment assembly.
The Repeatability of Clinical Measurements of Dry Eye (2004)

Although patient-reported symptoms are moderately repeatable from visit to visit, many of the procedures clinically used to diagnose and monitor dry eye syndromes are largely unrepeatable.
Guillain-Barré Syndrome (1985)

A study of the epidemiologic and clinical features of Guillain-Barré syndrome in the population of Olmsted County, Minnesota, over the 46-year period 1935 through 1980 was conducted through the centralized diagnostic index maintained at Mayo Clinic, Rochester, Minn. A total of 48 cases were identified, giving an age- and sex-adjusted incidence of 1.8 per 100,000 person-years. The rate increased over time from 1.2 in the interval 1935 through 1956 to 2.4 in the interval 1970 through 1980. Males were affected more than females (age-adjusted rates of 2.3 and 1.2, respectively). The rate increased with age from 0.8 in those under 18 years old to 3.2 for those 60 years and older. Antecedent infectious diseases were reported in 65% of the cases. Implications with regard to the incidence of Guillain-Barré syndrome associated with the A/New Jersey/76 (swine flu) vaccine are discussed.
Sprayed Polyelectrolyte Multilayers (2000)

Polyelectrolyte multilayers deposited by sequential spraying of poly(styrenesulfonate) and poly(diallyldimethylammonium) solutions are described. A highly uniform thin film is rapidly obtained over a large area. The morphology, uniformity, and chemical composition of sprayed multilayers, as well as the selective membrane properties, are found to be virtually identical to those prepared by dip-immersion.
A micro double capillary method for rheologic measurements of lower airway secretions. (1982)

We modified the double capillary method of Philipoff et al. [23] to allow rheologic measurements of lower respiratory secretions. The system consists of a precision bore stainless steel capillary (0.58 mm i.d., 5 mm length), a glass capillary (0.92 mm i.d., 15 mm length) and a sample holding tube (1.5 mm i.d., 15 mm length). This series of capillaries is connected to a long polyethylene catheter which permits advancing the system through the inner channel of a fiberoptic bronchoscope, and the placing of the sample holding tube into the lower airways for the aspiration of secretions under direct vision. The required sample volume is 20 microliter. The capillary is then removed from the fiberoptic bronchoscope and placed on a microscope stage; the movement of the meniscus of the sample under an applied vacuum as well as the elastic recoil of the sample after release of the vacuum are measured at body temperature. From this procedure, apparent viscosity (na) and shear elastic modulus (G) are calculated. The accuracy of the method was verified in vitro by using standard viscosity oils and a polymer solution of known elasticity. In five normal conscious sheep, na ranged between 200-3000 poises (shear rate: 0.06-1.0 s-1) and G between 17-83 dyn X cm-2. The micro double capillary method was found adequate for direct rheologic measurements of lower airway secretions.
SMN mRNA and protein levels in peripheral blood (2006)

SMN mRNA and protein levels can be reliably measured in the peripheral blood and used during clinical trials in spinal muscular atrophy, but these levels do not necessarily predict disease severity.
Rationally evolving tRNAPylfor efficient incorporation of noncanonical amino acids (2015)

Genetic encoding of noncanonical amino acids (ncAAs) into proteins is a powerful approach to study protein functions. Pyrrolysyl-tRNA synthetase (PylRS), a polyspecific aminoacyl-tRNA synthetase in wide use, has facilitated incorporation of a large number of different ncAAs into proteins to date. To make this process more efficient, we rationally evolved tRNA(Pyl) to create tRNA(Pyl-opt) with six nucleotide changes. This improved tRNA was tested as substrate for wild-type PylRS as well as three characterized PylRS variants (N(ϵ)-acetyllysyl-tRNA synthetase [AcKRS], 3-iodo-phenylalanyl-tRNA synthetase [IFRS], a broad specific PylRS variant [PylRS-AA]) to incorporate ncAAs at UAG codons in super-folder green fluorescence protein (sfGFP). tRNA(Pyl-opt) facilitated a 5-fold increase in AcK incorporation into two positions of sfGFP simultaneously. In addition, AcK incorporation into two target proteins (Escherichia coli malate dehydrogenase and human histone H3) caused homogenous acetylation at multiple lysine residues in high yield. Using tRNA(Pyl-opt) with PylRS and various PylRS variants facilitated efficient incorporation of six other ncAAs into sfGFP. Kinetic analyses revealed that the mutations in tRNA(Pyl-opt) had no significant effect on the catalytic efficiency and substrate binding of PylRS enzymes. Thus tRNA(Pyl-opt) should be an excellent replacement of wild-type tRNA(Pyl) for future ncAA incorporation by PylRS enzymes.
Contribution of NTFPs in the Livelihood of Mangrove Forest Dwellers of Sundarban (2010)

Sundarbans are the world’s largest mangrove biomes having highest mangrove biodiversity, which are used for subsistence and commercial purpose by the local inhabitants. It is one of the essential components of the livelihood of the forest dependant population of the area. The paper tries to explore the spectrum of NTFPs being collected from Sundarbans and its contribution in the livelihood of the people. It also attempts to assess the livelihood assets of the people and how much of it is attributed to NTFPs. It explores the livelihood vulnerability and stress coping mechanisms of the local population and how NTFPs help as a “safety net”. For the purpose of the study 4 EDCs from 2 ranges of Sundarbans were sampled, viz: Bally II and Bijoynagar, from Bidya Range; and Dulki and Hamiltanabad (Anpur) from Sajnakhali Wildlife Sanctuary Range. The parameters of the study included: (i) range of NTFPs being collected; (ii) seasonality and duration of collection; (iii) method of collection; (iv) extent of dependence; (v) total household income and contribution of NTFPs. The respondents were selected from different occupation class and income groups. The study shows that the contribution of NTFPs is quite high as it contributes almost 79% (Rs. 80,000) on an average to the annual income of the collector’s family. Of course not every family of the village goes for NTFP collection but nonetheless their number is significant. The major NTFP that are being collected includes firewood, prawn, fishes, crab, honey and bee wax. The collection of NTFPs is a daunting task, which involves risk from man-eater tigers. High livelihood vulnerability was also observed with little help from government. If, these biomes are to be conserved it is necessary that Sundarban Development Authority must consider the role of NTFPs in the livelihood and develop the Sustainable Livelihood Framework accordingly so that the biomes as well as people’s need could be sustained.
IL-6 Deficiency Attenuates Murine Diet-Induced Non-Alcoholic Steatohepatitis (2009)

Altogether, these results indicate that IL-6 deficiency does not block the development of NASH; yet, IL-6 plays a critical role in the accompanying liver inflammation.
Effective bisquaternary reactivators of tabun‐inhibited AChE (2005)

Two cholinesterase reactivators (K074 and K075) were synthesized and their reactivation efficacy against tabun-inhibited acetylcholinesterase of the rat brain was tested in vitro. Comparing this efficacy showed that commonly used oximes (pralidoxim, obidoxime and HI-6) were practically without reactivation potency. On the other hand, oximes K074, K075 and trimedoxime were satisfactorily effective. Moreover, K-oximes reactivated tabun-inhibited AChE at lower concentration (10(-4) and 10(-3) m) in comparison with trimedoxime (10(-3) and 10(-2) m). Thus, K-oximes can be considered as the most effective reactivators of tabun-inhibited AChE at present.
Fast mutually pumped phase conjugation using transient photorefractive coupling (1997)

Unusually fast mutually pumped phase-conjugation (MPPC) is achieved in a Bi12TiO20 crystal by using transient photorefractive beam coupling under a dc electric field. Pulses of phase-conjugate waves with a buildup time of 4.5 ms and a width of 50 ms at an input intensity of 2×0.8 W/cm2 are obtained after applying a rising front of external electric field to the crystal. A phase-conjugation efficiency as high as 25% of the incident light power is measured.
Role of a Highly Conserved Bacterial Protein in Outer Membrane Protein Assembly (2003)

After transport across the cytoplasmic membrane, bacterial outer membrane proteins are assembled into the outer membrane. Meningococcal Omp85 is a highly conserved protein in Gram-negative bacteria, and its homolog Toc75 is a component of the chloroplast protein-import machinery. Omp85 appeared to be essential for viability, and unassembled forms of various outer membrane proteins accumulated upon Omp85 depletion. Immunofluorescence microscopy revealed decreased surface exposure of outer membrane proteins, which was particularly apparent at the cell-division planes. Thus, Omp85 is likely to play a role in outer membrane protein assembly.