gebhart/patrepeval huggingface dataset:
Protein Adsorption on Poly(ethylene oxide)-Grafted Silicon Surfaces (1997)

Poly(ethylene oxide), in linear and star form, was covalently grafted to silicon surfaces in a range of grafting densities and the surfaces tested for their ability to adsorb proteins (cytochrome-c, albumin, fibronectin). The surfaces were analyzed by X-ray photoelectron spectroscopy (XPS) and ellipsometry, where both PEO content and amount of adsorbed protein were determined. All three proteins were found to reach zero adsorption at the highest grafting densities on all three linear PEG surfaces (PEG MW 3400, 10,000, and 20,000 g/mol). On both star PEO surfaces, albumin and fibronectin decreased to zero adsorption at intermediate grafting densities, whereas cytochrome-c continued to adsorb at all grafting densities. It was found that grafting densities of linear PEG in a brush regime were necessary to prevent protein adsorption. On PEO star surfaces, however, it was the decrease in space between grafted molecules to less than the size of the protein that was necessary in preventing the protein from adsorbing to the surface.
Intimal lesion formation in rat carotid arteries after endothelial denudation in absence of medial injury. (1990)

Injury of an artery by passage of a balloon catheter causes both endothelial denudation and medial damage and produces a marked smooth muscle cell (SMC) proliferative response. In this study, the endothelium from rat carotid arteries was removed by use of a rotating loop of 5/0 monofilament suture (gentle denudation technique), which did not cause any detectable damage to the underlying medial cells but did cause platelet adherence. Expression of platelet-derived growth factor (PDGF) A-chain and PDGF receptor mRNA was comparable to that seen in ballooned carotids, but the medial SMC proliferative response to gentle denudation was markedly reduced when compared to that observed after balloon denudation (1.4% vs. 13.6%). Intimal lesions were only observed in those zones that remained denuded for more than 7 days. These results demonstrate that a denuding injury with no medial trauma is sufficient to induce intimal lesions and that the significantly higher proliferation seen in ballooned vessels might reflect a response of the medial cells to trauma that occurred during denudation.
Staged Management of High-Energy Proximal Tibia Fractures (OTA Types 41) (2005)

This study supports the practice of delayed internal fixation until the soft-tissue envelope allows for definitive fixation.
Novel dual screen-dual film combination for mammography (1998)

A new dual screen-dual film mammography combination was constructed which made of two phosphor screens and two films loaded into a single x-ray cassette. The screens and films were combined so that a single emulsion film (Film #1, Kodak Min-R E film) was placed in direct contact with the phosphor side of the first screen (Screen #1). Screen #1 was made of the Kodak Min-R phosphor (34.0 mg/cm<SUP>2</SUP> Gd<SUB>2</SUB>O<SUB>2</SUB>S:Tb) coated on a 4 mil transparent Mylar backing. A double emulsion film (Film #2, Kodak T-Mat G film) was sandwiched between Screen #1 backing and the phosphor side of the second screen (Screen #2). Screen #2 was a Kodak Insight ME screen that has a Gd<SUB>2</SUB>O<SUB>2</SUB>S:Tb coating weight of 43.1 mg/cm<SUP>2</SUP> and a reflective coating between its phosphor layer and support layer. The relative sensitometric responses and spatial resolution properties of the two films were measured as a function of x-ray tube potential (kVp). A series of a contrast-detail phantom images was obtained by varying the x- ray exposure level at 28 kVp. An observer performance study was subsequently carried out to investigate the low contrast performance using the dual screen-dual film combination. The effective speeds of the two films differed by a factor of 2.12 to 2.67 between 25 to 30 kVp. Film #2 contrast was a factor of two or greater than Film #1 in the range where Film #1 optical density values were under 0.7. Measured MTF curves from digitized edge images showed that Film #1 MTF performance was comparable to a standard Kodak Min-R screen-Min-R E film combination. The limiting spatial resolution was found to be 19.5 lp/mm for Film #1 and approximately 11 lp/mm for Film #2. Observer performance studies showed that the threshold contrast in detecting small (less than 10 mm) breast lesions could be up to a factor of two lower on Film #2 images when regions of interest are underexposed on Film #1 images.
Effects of growth hormone treatment on the leptin system and on energy expenditure in abdominally obese men (1998)

The present study has examined the short- and long-term effects of growth hormone (GH) treatment on the leptin system and energy expenditure. Thirty male individuals with abdominal obesity were randomised to GH or placebo treatment in a 9-month, double-blind study. The dose of GH was 9.5 microg/kg, administered subcutaneously every evening. Serum leptin concentrations were measured by a human leptin RIA. Total RNA was isolated from adipose tissue biopsies and leptin mRNA levels were determined by a semi-quantitative reverse transcriptase-PCR assay. Body composition was determined by potassium-40 and the basal metabolic rate (BMR) was measured by a computerised, ventilated, open-hood system. As compared with placebo, an overall decrease in serum leptin concentrations as assessed by the area under the curve (AUC) (P < 0.05) and an increase in BMR (AUC, P < 0.05) were observed during GH treatment. The overall GH-induced changes were due to marked changes in serum leptin concentrations and BMR after 6 weeks of treatment. After 9 months of GH treatment there was a significant reduction in body fat (BF) while serum leptin concentrations and BMR did not differ from baseline values. Leptin mRNA levels did not change over the study period. We speculate that long-term GH treatment induces a new energy balance steady state with decreased BF stores. The effects of GH on the leptin system is suggested to be of importance for the maintenance of a lower BF mass.
N- and O-glycans of recombinant human C1 inhibitor expressed in the milk of transgenic rabbits (2003)

Human C1 inhibitor (hC1INH) is a therapeutic N, O-glycoprotein with a growing number of clinical applications, but the current natural supplies are not likely to meet the clinical demands. Therefore, recombinant approaches are of interest, whereby specific attention has to be paid to the generated glycosylation patterns. Here, the N,O-glycoprotein was expressed in the mammary gland of transgenic rabbits and subjected to glycan analysis. After release of the N-glycans of recombinant-rabbit human C1 inhibitor (rhC1INH) by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, the oligosaccharides were separated from the O-glycoprotein by centrifugal filtration, then fractionated by a combination of anion-exchange, normal-phase, and high-pH anion-exchange liquid chromatography. The O-glycans, released from the O-glycoprotein by alkaline borohydride treatment, were fractionated by anion-exchange high-performance liquid chromatography (HPLC). The structures of individual components were analysed by 500 MHz 1H NMR spectroscopy, in most cases combined with MALDI-TOF MS. In contrast to the structural data reported for native serum hC1INH, rhC1INH contained a broad array of different N-glycans, made up of oligomannose-, hybrid-, and complex-type structures. In the case of complex-type N-glycans (partially) (alpha2-6)-sialylated (N-acetylneuraminic acid only), mono- and diantennary chains were found; part of the diantennary structures were (alpha1-6)-core-fucosylated or (alpha1-3)-fucosylated in the lower or upper antenna (Lewis x). The manno-oligosaccharide pattern of part of the hybrid- and oligomannose-type structures indicates that besides the usual N-glycan processing route, also the alternative endo-mannosidase pathway is followed. The small core 1-type O-glycans showed the usual (alpha2-3)- and (alpha2-6)-sialylation pattern of O-glycoproteins of nonmucinous origin.
Enhanced circulatory parameters of human platelets cryopreserved with second‐messenger effectors: an in vivo study of 16 volunteer platelet donors (1999)

Platelet transfusion represents an important component of the therapy for thrombocytopenic patients. Prolonged storage capabilities for platelets would alleviate many problems associated with blood banking. Unfortunately, current cryopreservation methods are complex to implement and result in loss of cell number and functional activity. Previous in vitro studies have shown that the use of ThromboSolTM, a platelet-stabilizing formulation, in the cryopreservation of platelets results in significant retention of cell number and in vitro functional activities in addition to reducing the DMSO requirement to only 2%. We evaluated the in vivo circulatory parameters of platelets cryopreserved with ThromboSol. Single donor platelet units were obtained from healthy volunteers (n = 16); the units were then split and cryopreserved with either ThromboSol and 2% DMSO or 6% DMSO alone. Following storage at -80 degrees C for 7-10 d the samples were thawed, washed and radiolabelled with either 51Cr or 111In. The paired samples were then mixed and reinfused into the autologous volunteer. At various time intervals following transfusion a blood sample was drawn and the quantity of circulating labelled platelets was determined. The percent recovery and survival time was determined by multiple-hit analysis. The ThromboSol-treated platelets, as compared to the 6% DMSO-treated platelets, displayed statistically higher percent recovery (40.2% v 28.8%) and survival time (166.3 h v 152.1 h). These results demonstrated that platelets cryopreserved with ThromboSol displayed superior in vitro and in vivo characteristics as compared to the standard 6% DMSO method. The use of ThromboSol allowed for a 3-fold reduction in the DMSO concentration in conjunction with a 40% increase in circulating cell number and normal survival times.
Use of structural magnetic resonance imaging to predict who will get Alzheimer's disease (2000)

We used magnetic resonance imaging (MRI) measurements to determine whether persons in the prodromal phase of Alzheimer's disease (AD) could be accurately identified before they developed clinically diagnosed dementia. Normal subjects (n = 24) and those with mild memory difficulty (n = 79) received an MRI scan at baseline and were then followed annually for 3 years to determine which individuals subsequently met clinical criteria for AD. Patients with mild AD at baseline were also evaluated (n = 16). Nineteen of the 79 subjects with mild memory difficulty "converted" to a diagnosis of probable AD after 3 years of follow-up. Baseline MRI measures of the entorhinal cortex, the banks of the superior temporal sulcus, and the anterior cingulate were most useful in discriminating the status of the subjects on follow-up examination. The accuracy of discrimination was related to the clinical similarity between groups. One hundred percent (100%) of normal subjects and patients with mild AD could be discriminated from one another based on these MRI measures. When the normals were compared with the individuals with memory impairments who ultimately developed AD (the converters), the accuracy of discrimination was 93%, based on the MRI measures at baseline (sensitivity = 0.95; specificity = 0.90). The discrimination of the normal subjects and the individuals with mild memory problems who did not progress to the point where they met clinical criteria for probable AD over the 3 years of follow-up (the "questionables") was 85% and the discrimination of the questionables and converters was 75%. The apolipoprotein E genotype did not improve the accuracy of discrimination. The specific regions selected for each of these discriminations provides information concerning the hierarchical fashion in which the pathology of AD may affect the brain during its prodromal phase.
Detection of induced death of embryos in sheep by the rosette inhibition test (1979)

The rosette inhibition test has been used to monitor the decrease of an 'early pregnancy factor' in 2 groups of pregnant sheep (19-21 days) in which embryos were removed surgically or by induction of luteolysis with cloprostenol. The rosette inhibition titres of sera taken from sheep of each group declined from high (16-18) to low (8-10) levels within 48 h of treatment. Surgical removal of embryos caused little change in serum progesterone concentration whereas cloprostenol prompted a rapid decrease over the same period. Death of the embryo can therefore be detected by the rosette inhibition test within 48 h of occurrence, but not necessarily by the measurement of progesterone in blood within this period.
Direct Observation of Reversible Transformation of CH3NH3PbI3 and NH4PbI3 Induced by Polar Gaseous Molecules (2016)

Despite its competitive photovoltaic efficiency, the structural transformations of the prototypical hybrid perovskite, methylammonium lead iodide, are facilitated by interactions with polar molecules. Changes in optical and electronic properties upon exposure to ammonia potentially can enable the use of hybrid perovskites in gas-sensing applications. We investigated the effects of ammonia on CH<sub>3</sub>NH<sub>3</sub>PbI<sub>3</sub> by exposing perovskite films to a wide range of vapor pressures. Spectroscopic analyses indicated that ammonium cations replaced the methylammonium cations in the perovskite crystal, thereby resulting in the formation of NH<sub>4</sub>PbI<sub>3</sub>. The transformation of CH<sub>3</sub>NH<sub>3</sub>PbI<sub>3</sub> to NH<sub>4</sub>PbI<sub>3</sub> caused distinct changes in the morphology of the film and its crystalline structure; however, the introduction of CH<sub>3</sub>NH<sub>2</sub> gas reversed these changes. An in-depth understanding of the reversible chemical and structural alterations resulting from exposure to polar molecules can advance the development of hybrid perovskite sensors and provide insight into mechanisms by which perovskites convert due to interactions with polar molecules.
Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles (2012)

Curcumin has shown considerable pharmacological activity, including anti-inflammatory, but its poor bioavailability and rapid metabolization have limited its application. The purpose of the present study was to formulate curcumin-solid lipid nanoparticles (curcumin-SLNs) to improve its therapeutic efficacy in an ovalbumin (OVA)-induced allergic rat model of asthma. A solvent injection method was used to prepare the curcumin-SLNs. Physiochemical properties of curcumin-SLNs were characterized, and release experiments were performed in vitro. The pharmacokinetics in tissue distribution was studied in mice, and the therapeutic effect of the formulation was evaluated in the model. The prepared formulation showed an average size of 190 nm with a zeta potential value of -20.7 mV and 75% drug entrapment efficiency. X-ray diffraction analysis revealed the amorphous nature of the encapsulated curcumin. The release profile of curcumin-SLNs was an initial burst followed by sustained release. The curcumin concentrations in plasma suspension were significantly higher than those obtained with curcumin alone. Following administration of the curcumin-SLNs, all the tissue concentrations of curcumin increased, especially in lung and liver. In the animal model of asthma, curcumin-SLNs effectively suppressed airway hyperresponsiveness and inflammatory cell infiltration and also significantly inhibited the expression of T-helper-2-type cytokines, such as interleukin-4 and interleukin-13, in bronchoalveolar lavage fluid compared to the asthma group and curcumin-treated group. These observations implied that curcumin-SLNs could be a promising candidate for asthma therapy.
Regulation of mitochondrial fission and apoptosis by the mitochondrial outer membrane protein hFis1 (2005)

Mitochondrial fission is a highly regulated process mediated by a defined set of protein factors and is involved in the early stage of apoptosis. In mammals, at least two proteins, the dynamin-like protein DLP1/Drp1 and the mitochondrial outer membrane protein hFis1, participate in mitochondrial fission. The cytosolic domain of hFis1 contains six alpha-helices that form two tetratricopeptide repeat (TPR) motifs. Overexpression of hFis1 induces DLP1-mediated fragmentation of mitochondria, suggesting that hFis1 is a limiting factor in mitochondrial fission by recruiting cytosolic DLP1. In the present study, we identified two regions of hFis1 that are necessary for correct fission of mitochondria. We found that the TPR region of hFis1 participates in the interaction with DLP1 or DLP1-containing complex and that the first helix (alpha1) of hFis1 is required for mitochondrial fission presumably by regulating DLP1-hFis1 interaction. Misregulated interaction between DLP1 and hFis1 by alpha1 deletion induced mitochondrial swelling, in part by the mitochondrial permeability transition, but significantly delayed cell death. Our data suggest that hFis1 is a main regulator of mitochondrial fission, controlling the recruitment and assembly of DLP1 during both normal and apoptotic fission processes.
Noninvasive Transcutaneous Sampling of Glucose by Electroporation (2008)

Results of the present study demonstrate that ETS technique could be developed as a noninvasive method of predicting venous blood glucose levels in people with diabetes.
Automatic construction of 2D shape models (2001)

A procedure for automated 2D shape model design is presented. The system is given a set of training example shapes defined by contour point coordinates. The shapes are automatically aligned using Procrustes analysis and clustered to obtain cluster prototypes (typical objects) and statistical information about intracluster shape variation. One difference from previous methods is that the training set is first automatically clustered and shapes considered to be outliers are discarded. In this way, cluster prototypes are not distorted by outliers. A second difference is in the manner in which registered sets of points are extracted from each shape contour. We propose a flexible point matching technique that takes into account both pose/scale differences and nonlinear shape differences. The matching method is independent of the objects' initial relative position/scale and does not require any manually tuned parameters. Our shape model design method was used to learn 11 different shapes from contours that were manually traced in MR brain images. The resulting model was then employed to segment several MR brain images that were not included in the shape-training set. A quantitative analysis of our shape registration approach, within the main cluster of each structure, demonstrated results that compare very well to those achieved by manual registration; achieving an average registration error of about 1 pixel. Our approach can serve as a fully automated substitute to the tedious and time-consuming manual 2D shape registration and analysis.
Electronic Document Distribution (1994)

Computers, printers, and high-rate data-transmission facilities are becoming less expensive and more generally available. It is now possible to distribute customized newspapers and magazines electronically, instead of using a fleet of trucks and a network of vendors to disseminate a common paper version. A major obstacle to the use of electronic distribution is the ease of copying and redistributing electronic documents. This capability can affect a publisher's subscription revenues. The AT&T Distributed Systems Research Department is making it more difficult to redistribute electronic documents by: — Distinctive marking, so documents can be traced back to the original recipient; — Using cryptographic techniques, so the form of the document, available to a recipient, costs more to redistribute than that disseminated by the publisher; and — Requiring someone who redistributes a document to divulge personal, identifying information with the document. These techniques are being applied in experiments to distribute an issue of a technical journal electronically, and to mark and register paper copies of confidential executive memoranda.
Presence of electroencephalogram burst suppression in sedated, critically ill patients is associated with increased mortality (2008)

The presence of burst suppression, which was unexpectedly high in this medical intensive care unit population, was an independent predictor of increased risk of death at 6 months. This association should be studied prospectively on a larger scale in mechanically ventilated, critically ill patients.
Polymer Self-Assembly as a Novel Extension to Optical Lithography (2007)

The extreme technological complexity associated with continued dimensional scaling of the photolithographic patterning process to sub-50 nm dimensions has forced the semiconductor industry to seek increasingly innovative alternative approaches. One unconventional method under preliminary consideration involves using self-assembling block copolymer films as high-resolution patterning materials for defining integrated circuit (IC) elements. While these materials are attractive because of their ability to define nanometer-scale dimensions, their ultimate utility as a viable patterning method remains in question because of issues relating to pattern roughness and defectivity. In this issue, Prof. Paul Nealey and co-workers at the University of Wisconsin present compelling first demonstrations of experimental methods by which polymer self-assembly can generate the pattern elements essential for IC fabrication.
Larger Genetic Differences Within Africans Than Between Africans and Eurasians (2002)

The worldwide pattern of single nucleotide polymorphism (SNP) variation is of great interest to human geneticists, population geneticists, and evolutionists, but remains incompletely understood. We studied the pattern in noncoding regions, because they are less affected by natural selection than are coding regions. Thus, it can reflect better the history of human evolution and can serve as a baseline for understanding the maintenance of SNPs in human populations. We sequenced 50 noncoding DNA segments each approximately 500 bp long in 10 Africans, 10 Europeans, and 10 Asians. An analysis of the data suggests that the sampling scheme is adequate for our purpose. The average nucleotide diversity (pi) for the 50 segments is only 0.061% +/- 0.010% among Asians and 0.064% +/- 0.011% among Europeans but almost twice as high (0.115% +/- 0.016%) among Africans. The African diversity estimate is even higher than that between Africans and Eurasians (0.096% +/- 0.012%). From available data for noncoding autosomal regions (total length = 47,038 bp) and X-linked regions (47,421 bp), we estimated the pi-values for autosomal regions to be 0.105, 0.070, 0.069, and 0.097% for Africans, Asians, Europeans, and between Africans and Eurasians, and the corresponding values for X-linked regions to be 0.088, 0.042, 0.053, and 0.082%. Thus, Africans differ from one another slightly more than from Eurasians, and the genetic diversity in Eurasians is largely a subset of that in Africans, supporting the out of Africa model of human evolution. Clearly, one must specify the geographic origins of the individuals sampled when studying pi or SNP density.