gebhart/patrepeval huggingface dataset:
Phosphorus doping of epitaxial Si and Si1−xGex at very low pressure (1993)

We report on the in situ P doping of Si1−xGex(SiH4/GeH4) and Si(SiH4) epitaxial films with 1–100 ppm PH3 at 620 °C in a very low pressure chemical vapor deposition reactor. The epitaxial growth rates were found to decrease with P doping. The growth rate reduction is a function of Ge composition and is less pronounced as the Ge content increases. In addition, P incorporation into the growing film is affected by Ge, which improves the concentration range and profile definition of P. A simple model that attempts to explain the results is discussed.
Monolayer-Protected Au Cluster (MPC)-Supported Ti−BINOLate Complex (2003)

[reaction: see text] Disulfides bearing (R)-1,1'-bi-2-naphthol ((R)-BINOL) moieties at each terminal position have been successfully introduced on the surface of Au cluster. Ti-BINOLate complex generated from the obtained monolayer-protected Au cluster (MPC) promoted catalytic asymmetric alkylation of benzaldehyde with Et(2)Zn to afford the adduct in up to 98% yield with 86% ee. After completion of the reaction, the BINOL-functionalized MPC was easily recovered.
Cytologic examination of ovarian cyst fluid for the distinction between benign and malignant tumors. (1993)

The low sensitivity and negative predictive value of cytologic cyst fluid examination preclude its use for the distinction between benign and malignant ovarian tumors.
X-ray Laser (1988)

The x-ray sources now available are incoherent sources. When they are applied to x-ray diffraction, for example, only the intensity informations are used and phase informations are discarded. Therefore structure determination requires tremendous amount of times for measurements and analyses when the material has low degree of periodicity.When the x-ray laser, which is a coherent x-ray source, becomes available, the structure of the material even without having any periodicity can be uniquely determined with an x-ray holographic technique.Present status of x-ray laser development is reviewed in this paper.
A Convenient Solid-Phase Method for Synthesis of 3‘-Conjugates of Oligonucleotides (2001)

We present a new procedure for the preparation of 3'-conjugates of oligonucleotides through solid-phase synthesis. A suitable universal solid support was readily prepared using a series of peptide-like coupling reactions to incorporate first a spacer and then an L-homoserine branching unit. The N-alpha-position of the homoserine carries an Fmoc protecting group that is removed by treatment with piperidine to liberate an amino group suitable for attachment of the conjugate (e.g., small organic molecule, fluorescent group, cholesterol, biotin, amino acid, etc.) or for assembly of a short peptide. The side-chain hydroxyl group of the homoserine carries a trityl protecting group. After TFA deprotection, the hydroxyl group acts as the site for oligonucleotide assembly. An additional spacer, such as aminohexanoyl, may be incorporated easily between the conjugate molecule and the oligonucleotide. A number of examples of synthesis of 3'-conjugates of oligonucleotides and their analogues are described that involve standard automated oligonucleotide assembly and use of commercially available materials. The linkage between oligonucleotide and 3'-conjugate is chirally pure and is stable to conventional ammonia treatment used for oligonucleotide deprotection and release from the solid support. The homoserine-functionalized solid support system represents a simple and universal route to 3'-conjugates of oligonucleotides and their derivatives.
Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. (1995)

We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.
Recognizing nonadherence in patients with multiple sclerosis and maintaining treatment adherence in the long term. (2008)

The chronic nature of MS makes treatment adherence challenging in patients with long-standing disease. Patients and healthcare providers need to work together to establish open lines of communication and a trust-based therapeutic relationship to ensure that patients have the knowledge and skills they need to adhere to long-term MS therapy.
Human chorionic somatomammotropin and growth hormone gene expression in rat pituitary tumour cells is dependent on proximal promoter sequences (1989)

Human placental chorionic somatomammotropin (hCS-A or hCS-B) and pituitary growth hormone (hGH-N) are related by structure and function. The hCS-A gene is expressed in rat pituitary tumour (GC) cells after gene transfer. Deletion of hCS-A 5'-flanking DNA reveals repressor activity upstream of nucleotide -132, and a region essential for expression in GC cells between nucleotides -94 and -61. The sequences in this region differ from the equivalent hGH-N gene DNA by one nucleotide, and include the binding site (-92 to -65) for a pituitary-specific factor (GHF-1), required for hGH-N expression in GC cells. Exchange of hGH-N with hCS-A gene DNA in this region maintains expression in GC cells. By contrast, modification of these sequences blocks expression. These data indicate that proximal promoter sequences, equivalent to those bound by GHF-1 on the hGH-N gene, are required for hCS-A expression in GC cells.
System design of an Insertable Robotic Effector Platform for Single Port Access (SPA) Surgery (2009)

This paper presents a novel design and preliminary kinematic analysis of an insertable robotic effector platform (IREP) for single port access (SPA) surgery. The IREP robot can be deployed into body cavity through a Ø15 mm skin incision to perform SPA procedures. It consists of two snake-like continuum robots as slave surgical assistants for tissue manipulation, two parallelogram mechanisms for the continuum robots' placement, and one controllable stereo vision module with integrated light source for depth perception and tool tracking. Design considerations and alternatives, calculations and preliminary simulations of this 17-DoF surgical robotic system are presented in this paper. The overall control system hierarchy for tele-manipulation using the IREP robot is also presented.
Multilocus linkage analysis in humans: detection of linkage and estimation of recombination. (1985)

Multilocus linkage analysis is investigated from the viewpoint of the efficiency of recombination estimates under different strategies for detecting linkage and determining gene order within a linkage group. We consider the appropriateness of assuming no interference with data available in human genetic studies. Examples are given to show the significance of multilocus analysis in humans. A computer program package, LINKAGE, for multilocus linkage analysis is described.
In vitro evidence that Langerhans cells can adopt two functionally distinct forms capable of antigen presentation to T lymphocytes. (1989)

Monodisperse suspensions of epidermal Langerhans cells (LC) have been examined for their capacity to process and present Ag immediately upon extraction from mouse epidermis (fresh LC) and after 72 h in tissue culture (cultured LC). Cultured, but not fresh, LC stimulated proliferation among autologous T cells, whereas fresh, but not cultured, LC proved to be superior at processing native OVA for presentation to an OVA peptide-specific, MHC-restricted T cell hybridoma. Cultured LC were also more effective at stimulating proliferation among allogeneic T cells. However, a significant, but difficult to quantify, component of lymphocyte activation in these assays was derived from the ability of cultured LC to stimulate autologous T cells. It has been proposed that the superior capacity of cultured LC to stimulate T cells in these assays is due to the "immaturity" of freshly prepared LC--which "mature" during the 72-h culture interval. Based on the observation that fresh LC are superior at processing native protein Ag, we would amend the currently held notion that there is a "precursor-product" relationship between fresh and cultured LC to include the fact that these populations are differentially equipped to carry out distinct physiologic functions and that fresh LC should not, therefore, be considered "immature." We propose that fresh LC (in vitro equivalents of intraepidermal LC) can process native protein Ag with great efficiency, and can present these Ag in situ to memory and effector T cells (high affinity TCR interactions). Cultured LC (in vitro equivalents of LC that have migrated from skin to draining lymph node) exchange highly efficient Ag processing for acquisition of accessory molecules (surface ligands and secreted cytokines) that promote activation of unprimed T cells (including even low affinity TCR interactions).
Studies on Pest-Risk Involved in Import of Medicinal Plants (1989)

Germplasm of medicinal plants introduced through the Bureau comprises not only species of economic importance, but also of wild and related species. Tn order to know in advance as to which pests can come with the introduced material, crop-wise world literature was screened to compile the information on pests recorded, their nature of damage, life history, distribution, host range and susceptibility to the available disinfestation procedure and possible detection techniques. With this background information, an analysis was made to pinpoint the pest risk that can be present in the introduced material of Cassia, Chrysanthemum, Digitalis, Dioscorea, Glycyrrhiza, Hyoscyamus, Mentha, Olea, Papaver, Plantago, Rauvolfia, Vinca and Withania and in the likely event, how best to detect their presence at the time of quarantine examination, and finally how to liquidate them effectively and lower the quarantine risk so that the much needed medicinal germplasm could be made available to the users in India.
Heat Stability of the Lyophilized Sabin Poliovaccine (2003)

In this study we lyophilized three types of live attenuated polioviruses (Sabin vaccine strains) and evaluated the lyophilized vaccine viruses' heat stability. The virus titers were measured after heating at 37 and 45 and then compared with the titers of conventional liquid vaccine viruses similarly treated. The results showed that lyophilization, while slightly reducing vaccine virus titers, had a far greater sparing effect on subsequent heat inactivation of lyophilized vaccine viruses, thus demonstrating its validity for the improvement of the vaccine.
Multiplex Target Enrichment Using DNA Indexing for Ultra-High Throughput SNP Detection (2010)

Screening large numbers of target regions in multiple DNA samples for sequence variation is an important application of next-generation sequencing but an efficient method to enrich the samples in parallel has yet to be reported. We describe an advanced method that combines DNA samples using indexes or barcodes prior to target enrichment to facilitate this type of experiment. Sequencing libraries for multiple individual DNA samples, each incorporating a unique 6-bp index, are combined in equal quantities, enriched using a single in-solution target enrichment assay and sequenced in a single reaction. Sequence reads are parsed based on the index, allowing sequence analysis of individual samples. We show that the use of indexed samples does not impact on the efficiency of the enrichment reaction. For three- and nine-indexed HapMap DNA samples, the method was found to be highly accurate for SNP identification. Even with sequence coverage as low as 8x, 99% of sequence SNP calls were concordant with known genotypes. Within a single experiment, this method can sequence the exonic regions of hundreds of genes in tens of samples for sequence and structural variation using as little as 1 μg of input DNA per sample.
Human plasma carboxypeptidase N. Isolation and characterization. (1978)

Human plasma carboxypeptidase N has been purified 2,600-fold from pooled, outdated plasma in a 30% yield. Isolation was accomplished by chromatography on DEAE-cellulose and on a p-aminobenzoyl-L-arginine-Sepharose 6B affinity column. Carbohydrate accounts for 17% of the weight calculated from its amino acid and carbohydrate composition. The enzyme appears to consist of three subunits of Mr = 83,000, 55,000, and 49,000 and contains a significant amount of bound zinc. Purified enzyme preparations are very sensitive to proteolytic degradation but are stable for at least 3 months at 4 degrees.
Aberration of genomic DNA in association with human hepatocellular carcinomas detected by 2-dimensional gel analysis. (1994)

Alterations of genomic DNAs in primary hepatocellular carcinomas (HCCs) were examined by restriction landmark genomic scanning (I. Hatada et al., Proc. Natl. Acad. Sci. USA, 88: 9523-9527, 1991) which is a 2-dimensional gel analysis that allows detection of deletion, amplification, or other rearrangements of genomic DNA. Sixteen HCC samples together with their normal counterparts were tested in this manner. Each HCC sample was micromanipulated to minimize possible carryover from non-malignant cells. DNAs from HCCs and their normal counterparts were cleaved with the restriction enzyme NotI, end labeled with 32P, and size fractionated by 2-dimensional electrophoresis using HinfI as the second cleavage enzyme. The resulting spots (about 2000) in HCC samples were compared with their normal counterparts. Five spots were more intense in 10-14 of the 16 HCCs (63-88%). The intensity of several spots was reduced to about half, suggesting the loss of one of two alleles. Some of these decreases were observed frequently in different HCC samples, whereas others were sporadic. Sixty of these spots reproducibly decreased in > 2 cases, with 27 showing a decrease in > 50% of the informative cases. The highest incidence was observed in 14 of 16 samples (88%). No significant correlations were observed between these changes in spots and hepatitis B virus or hepatitis B virus infection. The use of landmarks that show a reproducible increase or decrease in intensity is discussed in conjunction with future studies of genomic alterations inherent in HCC.
Improved laser diode for high power and high temperature applications (2009)

The industry of laser marking, direct application and solid state laser pumping requires highly reliable and highly efficient laser diodes. In general, all applications demand improved brightness and temperature stability, and this by decreasing costs per watt. Instead of increasing the cavity length, we demonstrate in this paper an increase of power with standard cavity length with a clear focus of cost reduction and high efficiency. Improvements in the semiconductor material and packaging enable higher power and higher operation temperature. This technology raised the efficiency by 6 % of 808 nm bar with 50 % filling factor and a resonator length of 1.5 mm. Now, passively cooled diode lasers have reached nearly the performance of actively cooled ones. With this new design new fiber coupling modules with high brightness and high operation temperature for air cooled systems can be achieved.
Aminated Linear and Star-Shape Poly(glycerol methacrylate)s: Synthesis and Self-Assembling Properties (2010)

Over the past 10 years, polyglycerols and their structurally related analogs have received considerable attention in the biomedical field. Poly(glycidyl methacrylate) (PGMA) is a versatile polymer because its pendant epoxide groups can be opened with different functional groups to generate poly(glycerol methacrylate)s (PGOHMA) derivatives. In this work, linear and star-shape PGMAs were synthesized by atom transfer radical polymerization and then functionalized with four different amines by ring-opening addition. This resulted in the formation of polyglycerol-like polymers having both hydroxyl and amine moieties and different water-solubility. The water-insoluble polymers could form pH-sensitive nanoassemblies, while the soluble derivatives efficiently complexed a short strand polynucleotide. The aminated polyglycerol interacted more avidly with the oligonucleotide than the control poly(ethyleneimine), and high transfection efficacy could be obtained with the linear derivative. Such polymers could find practical applications for the delivery of drugs and nucleic acids.
A fantastic journey: 3D cardiac ultrasound goes live. (2003)

With a recent product introduction, live 3D echo is now clinically practical. It is already beginning to have a profound impact on the way we care for patients at The University of Chicago Medical Center. In the past, dynamic cardiac 3D rendered images were possible by sequentially acquiring 2D images and then using a workstation to input 2D images for Cartesian coordinate conversion and volume rendering. Outside research settings, this time-consuming process proved cumbersome and was simply impractical. Now that these technical and practical issues have been addressed, real-time 3D cardiac sonography has great potential to impact both patient care and throughput in a number of ways, including better pre- and post-surgical planning, improved measurement of heart function, decreased exam times, and enhanced communication between clinicians and their patients. With real-time 3D cardiac ultrasound images, clinicians will be able to better quantify size, shape and function of the heart. However, the most important contribution of real-time 3D sonography in cardiology may be improvement in locating abnormalities for surgical planning. The new technology will also provide important information regarding surgical outcomes. A great benefit to obtaining more diagnostic information and higher diagnostic confidence from real-time 3D cardiac ultrasound images is that it could lead to more rapid exam times and the reduction of patient wait times. Being able to see the whole heart makes examinations more simple and rapid, benefitting the staff and patient. The utility of this technology is unusually broad, as it is able to move beyond diagnostics into a key role in therapeutic procedures. As with any new technology, there will be a learning curve to understanding 3D imaging. Though the matrix transducer is somewhat larger than a standard 2D probe, the ergonomics are quite similar. The interface of the ultrasound unit is also very user friendly. Because real-time 3D cardiac ultrasound involves looking at the heart as if you are holding it in your hands, with the additional ability to turn it any way you want, we expect that the transition from 2D to 3D will be easily achieved.
Inducible Resistance of Tumor Cells to Tumor Necrosis Factor–Related Apoptosis-Inducing Ligand Receptor 2–Mediated Apoptosis by Generation of a Blockade at the Death Domain Function (2006)

Induction of tumor cell resistance to therapeutics has been a major obstacle in cancer therapy. Targeting of the death receptors by a natural ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), or agonistic monoclonal antibodies against TRAIL receptor 1 (TRAIL-R1) or TRAIL receptor 2 (TRAIL-R2) has been thought to be a promising cancer therapy. To determine whether tumor cells are able to generate a resistance to apoptosis induced by an anti-TRAIL-R2 antibody, TRA-8, we examined the apoptotic response of human breast and ovarian cancer cell lines after treatment with TRA-8. Our results show that tumor cell resistance to TRA-8 can be induced by repeated treatment of tumor cells with low, non-apoptosis-inducing doses of TRA-8. Interestingly, the induced resistance to apoptosis was not due to a global apoptotic defect in tumor cells but rather a selective defect in the TRAIL-R2 signaling pathway. Whereas TRA-8-treated tumor cells developed a selective resistance to TRAIL-R2-mediated apoptosis, the apoptotic responses induced by TRAIL, an anti-TRAIL-R1 antibody (2E12), and other apoptotic stimuli were not impaired. The expression levels of cell surface TRAIL-R2 were not altered and mutations of TRAIL-R2 were not found in the resistant cells. The induced TRA-8 resistance was due to a selective blockade at the level of the death domain and could be reversed by a wide array of chemotherapeutic agents. Proteomic analysis of death-inducing signaling complex formation during TRA-8 treatment shows that the translocation of TRAIL-R2-associated apoptotic proteins was significantly altered. Our results suggest that the prevention of tumor cell resistance to therapeutic agents that target the death receptors must be taken into consideration.
Virus‐like particles induce robust human T‐helper cell responses (2011)

Among synthetic vaccines, virus-like particles (VLPs) are used for their ability to induce strong humoral responses. Very little is reported on VLP-based-vaccine-induced CD4(+) T-cell responses, despite the requirement of helper T cells for antibody isotype switching. Further knowledge on helper T cells is also needed for optimization of CD8(+) T-cell vaccination. Here, we analysed human CD4(+) T-cell responses to vaccination with MelQbG10, which is a Qβ-VLP covalently linked to a long peptide derived from the melanoma self-antigen Melan-A. In all analysed patients, we found strong antibody responses of mainly IgG1 and IgG3 isotypes, and concomitant Th1-biased CD4(+) T-cell responses specific for Qβ. Although less strong, comparable B- and CD4(+) T-cell responses were also found specific for the Melan-A cargo peptide. Further optimization is required to shift the response more towards the cargo peptide. Nevertheless, the data demonstrate the high potential of VLPs for inducing humoral and cellular immune responses by mounting powerful CD4(+) T-cell help.
Surface-Initiated Photopolymerization of Poly(ethylene glycol) Methyl Ether Methacrylate on a Diethyldithiocarbamate-Mediated Polymer Substrate (2002)

Grafting efficiency and graft conversion have been investigated for poly(ethylene glycol) methyl ether methacrylate (m-PEGMA) polymerized on the surface of diethyldithiocarbamate-containing polymer substrates. The substrate is prepared by copolymerization of a mixture of methacrylic monomers with a methacrylic diethyldithiocarbamate molecule, which serves as a photoiniferter that is chemically anchored on the surface of and throughout the substrate. Surface initiation was revealed by FTIR measurements of m-PEG200MA monomer conversion for two different monomer layer thicknesses; however, side reactions including chain transfer to PEG units affect surface-initiated polymerization of m-PEG200MA monomer significantly. Chain transfer causes a sharp decrease in the measured grafting efficiency at the beginning of this surface-mediated polymerization. Addition of N,N,N‘,N‘-tetraethylthiuram disulfide (TED), which suppresses chain transfer to PEG units, and the use of octyl methacrylate as the grafting monomer result in an increase in graft efficiency at early stages of the polymerization. Specific polymerization events that relate the chain transfer of PEG units to the graft properties of the photoiniferter-mediated polymerization are discussed.
Low resistance Ti/Au contacts to amorphous gallium indium zinc oxides (2011)

We report on low-resistance Ti (50 nm)/Au (80 nm) contacts to amorphous gallium indium zinc oxides (a-GIZO). The specific contact resistances obtained using the transmission line method were as low as 2.85×10−5 Ω cm2 when annealed at 500 °C for 1 min in N2 ambient. This could be attributed to the combined effects of structural relaxation of a-GIZO films at elevated temperatures, causing drastic increases in both electron concentration and Hall mobility, and to interfacial reactions between Ti/Au and a-GIZO layers producing oxygen vacancies near the surface.