gebhart/patrepeval huggingface dataset:
Influence of nutritional status and oestradiol-17β on plasma growth hormone, insulin-like growth factors-I and -II and the response to exogenous growth hormone in young steers (1988)

Plasma GH profiles and circulating concentrations of plasma insulin-like growth factors-I and -II (IGF-I and -II) were examined in 20 steers on either high (3% dry matter of body weight per day) or low (1% dry matter of body weight per day) planes of nutrition with or without an implant of oestradiol-17 beta. The response of plasma IGF-I and -II to a bolus injection of bovine GH (bGH) was also investigated. Reduced feeding significantly (P less than 0.01) increased the mean concentration, peak height and integrated area of plasma GH. Treatment of steers with oestradiol at low nutrition significantly increased baseline GH concentrations. Treatment of steers with oestradiol at high nutrition significantly (P less than 0.05) increased mean, baseline, peak height, and integrated area of plasma GH. GH pulse frequency was not changed by either nutritional plane or oestradiol treatment. Basal concentrations of plasma IGF-I were significantly (P less than 0.01) decreased by reduced feeding in both the oestradiol-treated and the control group. Treatment with oestradiol increased (P less than 0.01) basal plasma concentrations of IGF-I at both high and low levels of nutrition. After i.v. injection of bGH (0.1 mg/kg body weight), an increase in plasma IGF-I was observed only in steers at high nutrition. Basal concentrations of plasma IGF-II were not altered by nutritional manipulations but were significantly (P less than 0.001) increased by oestradiol treatment. After bGH infusion only steers at high nutrition showed an increase in plasma IGF-II.(ABSTRACT TRUNCATED AT 250 WORDS)
Implanted Electrochemical Glucose Sensors for the Management of Diabetes (1999)

By maintaining a near normal (70-120 mg/dL) glucose concentration, diabetic patients can drastically reduce the likelihood of the occurrence of diabetes complications. In the near future, subcutaneously implanted electrochemical glucose sensors will be available to provide frequent or continuous information on which timely treatment decisions, such as insulin injection or glucose source intake, can be based, as well as timely alarm signals. The currently engineered devices are of three types: (a) innocuous microsensors, with actively mass-transporting areas < 10(-3) cm2, replaced twice a week by the patient; (b) self-contained, surgeon-implanted, transmitter-containing packages of > 1 cm2 area, operating for > 100 days; and (c) devices transporting subcutaneous fluid to an external sensor, based on implanted microfiltration or microdialysis fibers or on iontophoretic transport of the subcutaneous fluid through the skin.
Deletion of the varicella-zoster virus large subunit of ribonucleotide reductase impairs growth of virus in vitro (1994)

Cells infected with varicella-zoster virus (VZV) express a viral ribonucleotide reductase which is distinct from that present in uninfected cells. VZV open reading frames 18 and 19 (ORF18 and ORF19) are homologous to the herpes simplex virus type 1 genes encoding the small and large subunits of ribonucleotide reductase, respectively. We generated recombinant VZV by transfecting cultured cells with four overlapping cosmid DNAs. To construct a virus lacking ribonucleotide reductase, we deleted 97% of VZV ORF19 from one of the cosmids. Transfection of this cosmid with the other parental cosmids yielded a VZV mutant with a 2.3-kbp deletion confirmed by Southern blot analysis. Virus-specific ribonucleotide reductase activity was not detected in cells infected with VZV lacking ORF19. Infection of melanoma cells with ORF19-deleted VZV resulted in plaques smaller than those produced by infection with the parental VZV. The mutant virus also exhibited a growth rate slightly slower than that of the parental virus. Chemical inhibition of the VZV ribonucleotide reductase has been shown to potentiate the anti-VZV activity of acyclovir. Similarly, the concentration of acyclovir required to inhibit plaque formation by 50% was threefold lower for the VZV ribonucleotide reductase deletion mutants than for parental virus. We conclude that the VZV ribonucleotide reductase large subunit is not essential for virus infection in vitro; however, deletion of the gene impairs the growth of VZV in cell culture and renders the virus more susceptible to inhibition by acyclovir.
Linear network coding (2003)

Consider a communication network in which certain source nodes multicast information to other nodes on the network in the multihop fashion where every node can pass on any of its received data to others. We are interested in how fast each node can receive the complete information, or equivalently, what the information rate arriving at each node is. Allowing a node to encode its received data before passing it on, the question involves optimization of the multicast mechanisms at the nodes. Among the simplest coding schemes is linear coding, which regards a block of data as a vector over a certain base field and allows a node to apply a linear transformation to a vector before passing it on. We formulate this multicast problem and prove that linear coding suffices to achieve the optimum, which is the max-flow from the source to each receiving node.
Dynamic changes of gene expression profiles during postnatal development of the heart in mice (2004)

Global gene expression analysis in the whole heart may be useful in understanding the orchestrated process of postnatal development or terminal differentiation in the cardiac environment. These data are likely to be helpful in studying developmental anomalies of the heart in neonates.
Vasoconstrictive agents commonly used in combination with local anesthetics: a literature review. (1987)

A review of the current medical literature concerning the use of various vasoconstrictive agents with local anesthetics is presented. These agents are employed in podiatry primarily for the purpose of prolonging the duration of anesthesia and surgical hemostasis. Epinephrine, phenylephrine, levonordefrin, felypressin, and norepinephrine have all been utilized in conjunction with the local anesthetics by the various medical professions. Although controversy surrounds the use of these agents, this article should assist the podiatric physician in making an informed decision.
Differential Distribution of Dynamin Isoforms in Mammalian Cells (1998)

Dynamins are 100-kDa GTPases that are essential for clathrin-coated vesicle formation during receptor-mediated endocytosis. To date, three different dynamin genes have been identified, with each gene expressing at least four different alternatively spliced forms. Currently, it is unclear whether these different dynamin gene products perform distinct or redundant cellular functions. Therefore, the focus of this study was to identify additional spliced variants of dynamin from rat tissues and to define the distribution of the dynamin family members in a cultured rat epithelial cell model (Clone 9 cells). After long-distance reverse transcription (RT)-PCR of mRNA from different rat tissues, the full-length cDNAs encoding the different dynamin isoforms were sequenced and revealed four additional spliced variants for dynamin I and nine for dynamin III. Thus, in rat tissues there are a total of at least 25 different mRNAs produced from the three dynamin genes. Subsequently, we generated stably transfected Clone 9 cells expressing full-length cDNAs of six different spliced forms tagged with green fluorescent protein. Confocal or fluorescence microscopy of these transfected cells revealed that many of the dynamin proteins associate with distinct membrane compartments, which include clathrin-coated pits at the plasma membrane and the Golgi apparatus, and several undefined vesicle populations. These results indicate that the dynamin family is more extensive than was originally predicted and suggest that the different dynamin proteins are localized to distinct cytoplasmic or membrane compartments.
Obtaining full-diversity space-frequency codes from space-time codes via mapping (2003)

This paper considers the problem of space-frequency code design for frequency-selective multiple-input-multiple-output (MIMO) orthogonal frequency division multiplexing (OFDM) modulation. We show that space-time codes achieving full diversity in quasistatic flat fading environment can be used to construct space-frequency codes that can achieve the maximum diversity available in frequency-selective MIMO fading channels. Since the codes are constructed via a simple mapping from space-time codes to space-frequency codes, the abundant classes of existing space-time block and trellis codes can be used for full diversity transmission in MIMO-OFDM systems. The proposed mapping provides a tradeoff between the achieved diversity order and the symbol rate. Moreover, we characterize the performance of the space-frequency codes obtained via the mapping by finding lower and upper bounds on their coding advantages as functions of the coding advantages of the underlying space-time codes. This result will allow us to investigate the effects of the delay distribution and the power distribution of the channel impulse responses on the performance of the resulting space-frequency codes. Extensive simulation results are also presented to illustrate and support the theory.
Recombinant antibody fragments and immunotoxin fusions for cancer therapy. (2000)

Recombinant immunotoxins consist of Fv regions of cancer specific antibodies fused to truncated bacterial toxins. Many recombinant immunotoxins contain a truncated version of Pseudomonas Exotoxin as a toxic moiety. This toxin is modified in such a manner that by itself it does not bind to normal human cells, but it retains all other functions of cytotoxicity. The recombinant antibody fragments target the modified toxin to cancer cells which are killed, either by direct inhibition of protein synthesis, or by concomitant induction of apoptosis. Cells that are not recognized by the antibody fragment, because they do not carry the cancer antigen, are spared. Many factors influence the in vivo anti-tumor activity of recombinant immunotoxins. Among them are considerations of which types of cancer and at what stages may be the best targets for immunotoxin therapy and tumor specificity of the antigen that is targeted by the recombinant antibody. Also the affinity of immunotoxins and their ability to enter and penetrate into tissues and tumors, which in turn is dependent on the size of the protein. And, because one very important factor is the stability of immunotoxins, a great deal of protein-engineering is required to stabilize the recombinant antibody moiety of immunotoxins. Excellent activity and specificity can be observed for many recombinant immunotoxins in in vitro assays using cultured cancer cells as well as in animal tumor models. Ongoing clinical trials provide examples where the promising preclinical data correlate with successful results in experimental cancer therapy.
ATP-Binding Site of Human Brain Hexokinase As Studied by Molecular Modeling and Site-Directed Mutagenesis (1996)

The interaction of ATP with the active site of hexokinase is unknown since the crystal structure of the hexokinase-ATP complex is unavailable. It was found that the ATP binding site of brain hexokinase is homologous to that of actin, heat shock protein hsc70, and glycerol kinase. On the basis of these similarities, the ATP molecule was positioned in the catalytic domain of human brain hexokinase, which was modeled from the X-ray structure of yeast hexokinase. Site-directed mutagenesis was performed to test the function of residues presumably involved in interaction with the tripolyphosphoryl moiety of ATP. Asp532, which is though to be involved in binding the Mg2+ ion of the MgATP2- complex, was mutated to Lys and Glu. The kcat values decreased 1000- and 200-fold, respectively, for the two mutants. Another residue, Thr680 was proposed to interact with the gamma-phosphoryl group of ATP through hydrogen bonds and was mutated to Val and Ser. The kcat value of the Thr680Val mutant decreased 2000-fold, whereas the kcat value of the Thr680Ser decreased only 2.5-fold, implying the importance of the hydroxyl group. The Km and dissociation constant values for either ATP or glucose of all the above mutants showed little or no change relative to the wild-type enzyme. The Ki values for the glucose 6-phosphate analogue 1,5-anhydroglucitol 6-phosphate, were the same as that of the wild-type enzyme, and the inhibition was reversed by inorganic phosphate (Pi) for all four mutants. The circular dichroism spectra of the mutants were the same as that of the wild-type enzyme. The results from the site-directed mutagenesis demonstrate that the presumed interactions of investigated residues with ATP are important for the stabilization of the transition state.
Tinkering with Enzymes: What Are We Learning? (1987)

It is now possible, by site-directed mutagenesis of the gene, to change any amino acid residue in a protein to any other. In enzymology, application of this technique is leading to exciting new insights both into the mechanism of catalysis by particular enzymes, and into the basis of catalysis itself. The precise and often delicate changes that are being made in and near the active sites of enzymes are illuminating the interdependent roles of catalytic groups, and are allowing the first steps to be taken toward the rational alteration of enzyme specificity and reactivity.
Epidermal Growth Factor Induces Differentiation and Secretion of Human Chorionic Gonadotropin and Placental Lactogen in Normal Human Placenta* (1987)

Human trophoblast differentiates by the fusion of cytotrophoblasts to form syncytiotrophoblast. To determine factors controlling this process, the effects of epidermal growth factor (EGF) on trophoblast differentiation were studied using long term serum-free culture of isolated trophoblast. Only trophoblast was present in the cultures, as demonstrated by positive immunoperoxidase staining with beta hCG, cytokeratin, and trophoblast-specific H315 monoclonal antisera and by the absence of contaminating endothelial cells, fibroblasts, and macrophages, as shown by negative staining with vimentin and OKM1 monoclonal antisera. EGF induced large sustained increases in hCG and human placental lactogen (hPL) secretion in a dose-dependent manner. The minimum effective dose was 0.1 ng/mL, and the maximum effective dose was 1 ng/mL. Light and electron microscopic studies showed EGF-induced differentiation of cytotrophoblast to form syncytiotrophoblast. DNA content and cell number did not change during the process. The formation of syncytia thus probably accounted for the increase in hCG and hPL secretion. We conclude that EGF causes morphological differentiation, but not cell proliferation, of trophoblasts, and the differentiation results in increased hCG and hPL secretion from the syncytia.
A QSAR investigation of dihydrofolate reductase inhibition by Baker triazines based upon molecular shape analysis (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA QSAR investigation of dihydrofolate reductase inhibition by Baker triazines based upon molecular shape analysisA. J. HopfingerCite this: J. Am. Chem. Soc. 1980, 102, 24, 7196–7206Publication Date (Print):November 19, 1980Publication History Published online1 May 2002Published inissue 19 November 1980https://pubs.acs.org/doi/10.1021/ja00544a005https://doi.org/10.1021/ja00544a005research-articleACS PublicationsRequest reuse permissionsArticle Views336Altmetric-Citations206LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Actual Causality (2016)

Causality plays a central role in the way people structure the world; we constantly seek causal explanations for our observations. But what does it even mean that an event C actually event E? The problem of defining actual causation goes beyond mere philosophical speculation. For example, in many legal arguments, it is precisely what needs to be established in order to determine responsibility. The philosophy literature has been struggling with the problem of defining causality since Hume. In this book, Joseph Halpern explores actual causality, and such related notions as degree of responsibility, degree of blame, and causal explanation. The goal is to arrive at a definition of causality that matches our natural language usage and is helpful, for example, to a jury deciding a legal case, a programmer looking for the line of code that cause some software to fail, or an economist trying to determine whether austerity caused a subsequent depression. Halpern applies and expands an approach to causality that he and Judea Pearl developed, based on structural equations. He carefully formulates a definition of causality, and building on this, defines degree of responsibility, degree of blame, and causal explanation. He concludes by discussing how these ideas can be applied to such practical problems as accountability and program verification. Technical details are generally confined to the final section of each chapter and can be skipped by non-mathematical readers.
ELF: efficient lightweight fast stream processing at scale (2014)

Large Internet companies like Facebook, Amazon, and Twitter are increasingly recognizing the value of stream data processing, using tools like Flume, Muppet, or Storm to continuously collect and process incoming data in real time to help govern company activities. Applications include monitoring marketing streams for business-critical decisions, identifying spam campaigns from social network streams, datacenter's intrusion detection and troubleshooting, and others. Technical challenges for stream processing include the following: how to scale to numerous, concurrently running streaming jobs, to coordinate across those jobs to share insights, to make online changes to job functions to adapt to new requirements or data characteristics, and for each job, to efficiently operate over different time windows. This dissertation presents a new stream processing model, termed ELF, which addresses these new challenges. ELF proposes a novel decentralized "many masters many workers'' architecture implemented over a set of agents enriching the web tier of datacenter systems. ELF uses a DHT protocol to assign the jobs respective sets of master/workers mapping to the agents of the web tier, where for each job, the live data streams generated by webservers are first divided into mini-batches, then inserted and aggregated as space-efficient compressed buffer trees (CBTs) in local agents' memories. Second, per-batch results are `flushed' from CBTs, to be rolled up and aggregated via shared reducer trees (SRTs), in ways that naturally balance SRT-induced load, reduce processing latencies, and allow online job changes along with cross-job coordination. An ELF prototype implemented and evaluated for a larger scale configuration demonstrates scalability, high per-node throughput, sub-second job latency, and sub-second ability to adjust the actions of jobs being run.
Learning communicative actions of conflicting human agents (2008)

One of the main problems to be solved while assisting inter-human conflict resolution is how to reuse the previous experience with similar agents. A machine learning technique for handling scenarios of interaction between conflicting human agents is proposed. Scenarios are represented by directed graphs with labelled vertices (for communicative actions) and arcs (for temporal and causal relationships between these actions and their parameters). For illustrative purposes, classification of a scenario is computed by comparing partial matching of its graph with graphs of positive and negative examples. Nearest Neighbour learning is followed by the JSM-based learning which minimised the number of false negatives and takes advantage of a more accurate way of matching sequences of communicative actions. Developed scenario representation and comparative analysis techniques are applied to the classification of textual customer complaints. It is shown that analysing the structure of communicative actions without context information is frequently sufficient to estimate complaint validity. Therefore, being domain-independent, proposed machine learning technique is a good compliment to a wide range of customer relation management applications where formal treatment of inter-human interactions is required in a decision-support mode.
Development of a Neuropathic Pain Questionnaire (2003)

Ongoing efforts to develop mechanisms-based assessment and treatment of chronic pain have been hindered by the lack of assessment tools differentially sensitive to various phenomena underlying different mechanisms of pain. This study describes the development of an assessment instrument intended to measure neuropathic pain based on qualities of pain as they are inferred from pain descriptors. Subjects were 528 chronic pain patients from several clinics. Of these, 149 had strictly neuropathic pain, while 233 had non-neuropathic pain. Subjects completed a 32 item preliminary questionnaire, which asked them to rate their usual pain on multiple descriptors, as well as the degree to which their pain differed in response to various internal and external factors. This preliminary questionnaire was submitted to factor analysis, and this yielded 6 factors. Representatives of each of these factors were combined with additional items that demonstrated significant differences between neuropathic and non-neuropathic pain groups, to yield a 12 item Neuropathic Pain Questionnaire (NPQ). These items were able to differentiate neuropathic pain patients from non-neuropathic pain patients in a holdout sample with 66.6% sensitivity and 74.4% specificity. The newly developed instrument, NPQ, may be used for the initial screening of neuropathic pain patients. It also has the ability to provide a quantitative measure for the descriptors important in the diagnosis and assessment of neuropathic pain. Consequently, it can be used for monitoring of neuropathic pain treatments and as an outcome measure.