gebhart/patrepeval huggingface dataset:
RT-Plant Physiology: Full Open Access Publishing at No Charge to ASPB Members (2006)

to the print versions of both Plant Physiology and The Plant Cell, free electronic access to Plant Physiology and The Plant Cell, and a discount on registration fees for ASPB meetings.Annual membership in the American Society of Plant Biologists is $115 for regular members (http://www.aspb.org/membership/) and considerably less for postdocs and students.Additionally, beginning with articles submitted October 1, 2006, free online color will be offered to all Plant Physiology authors.With the online use of Plant Physiology growing at a rapid rate, online color is becoming evermore important.This new feature will allow you to have color images in the online version of your article and black and white in the print version for no charge.Online-only color adds value when color is not critical for data interpretation but aids in presentation.Why should you be concerned whether or not your article is published with Open Access?In addition to helping fulfill the altruistic academic aspiration of making new knowledge as widely available as possible, there are strong reasons to believe that Open Access drives higher impact and citation by accelerating recognition and dissemination of research findings.A recent longitudinal bibliometric analysis of Open Access vs. non-Open Access papers published over a
"Libraries from libraries": chemical transformation of combinatorial libraries to extend the range and repertoire of chemical diversity. (1994)

The generation of diverse chemical libraries using a "libraries from libraries" concept is described. The central features of the approaches presented are the use of well-established solid-phase synthesis methods for the generation of combinatorial libraries, combined with the chemical transformation of such libraries while they remain attached to the solid support. The chemical libraries that are generated by this process have very different physical, chemical, and biological properties compared to the libraries from which they were derived. A wide range of chemical transformations are possible for peptide-based or other libraries, and an almost unlimited range of useful chemical diversities can be envisioned. In the example presented, the amide functionalities in an existing combinatorial library made up of peptides were permethylated while the library remained attached to the solid-phase support used in its synthesis. After removal of the permethylated mixtures from their solid support, this library, now lacking the typical -CONH- amide bonds of peptides, can be tested in solution with virtually all existing assay systems to identify individual compounds having specific biological activities of interest. An illustration of the use of such libraries is presented, in which the described permethylated library was used to identify individual permethylated compounds having potent antimicrobial activity against Gram-positive bacteria.
Anxiolytic- and antidepressant-like effects of an antagonist at glycineB receptors. (1999)

L-701,324 [7-chloro-4-hydroxy-3-(3-phenoxy)phenyl-2-(1H)-quinolone], a selective antagonist at glycineB receptors, was studied in behavioral tests used to predict a potential anxiolytic (conflict drinking test in rats, four-plate test in mice) and antidepressant (forced swimming test in rats and mice) activity. In the conflict drinking test in rats, L-701,324 (0.5 mg/kg, but not 0.25 and 1 mg/kg) increased the number of punished licks in a statistically significant manner. In the four-plate test in mice, L-701,324 (2 mg/kg, but no lower doses) significantly increased the number of punished crossings. In the forced swimming test in rats, L-701,324 (0.5 mg/kg) slightly, but statistically significantly, reduced the immobility time; the drug was inactive in doses of 0.25 and 1 mg/kg. In mice, L-701,324 administered only in the highest dose used (2 mg/kg) significantly shortened the immobility time. L-701,324 (given in doses higher then 2 mg/kg) induced motor impairment in animals. The above findings indicate that L-701,324 shows weak anxiolytic- and antidepressant-like activities in the animal models used.
Silanation of silica surfaces. A new method of constructing pure or mixed monolayers (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilanation of silica surfaces. A new method of constructing pure or mixed monolayersP. Silberzan, L. Leger, D. Ausserre, and J. J. BenattarCite this: Langmuir 1991, 7, 8, 1647–1651Publication Date (Print):August 1, 1991Publication History Published online1 May 2002Published inissue 1 August 1991https://pubs.acs.org/doi/10.1021/la00056a017https://doi.org/10.1021/la00056a017research-articleACS PublicationsRequest reuse permissionsArticle Views4293Altmetric-Citations450LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Modulation of expression of delayed hypersensitivity by mycobacterial antigen 85 fibronectin-binding proteins (1992)

Although demonstration of delayed hypersensitivity to purified protein derivative of tuberculin (PPD) is an important element in the diagnosis of infection with Mycobacterium tuberculosis, many patients with tuberculosis are anergic. Several possible mechanisms for this specific lack of response have been described. We have now uncovered an additional one. T-cell fibronectin (FN), a lymphokine secreted by activated T cells, is closely associated with the initiation of delayed hypersensitivity reactions. Mycobacterial antigen 85 (Ag85) proteins have been shown to bind to plasma FN. The ability of Ag85 to bind to T-cell FN and modulate expression of delayed hypersensitivity was therefore studied. Purified Ag85 proteins from M. tuberculosis, Mycobacterium bovis BCG, or Mycobacterium kansasii bound to T-cell FN, fibroblast FN, and plasma FN in vitro. Purified 65-kDa heat shock protein (hsp65) from M. bovis BCG did not bind to any FN. Ag85, but not hsp65, inhibited the ability of T-cell FN to agglutinate monocytes in vitro in a dose-dependent manner. In vivo, mixtures of PPD or dinitrophenyl-ovalbumin and purified M. tuberculosis or M. bovis BCG Ag85 proteins elicited significantly smaller delayed hypersensitivity inflammatory reactions in sensitized guinea pigs than did PPD or dinitrophenyl-ovalbumin alone. Purified hsp65 did not inhibit expression of delayed hypersensitivity to PPD or dinitrophenyl-ovalbumin. We suggest that Ag85 proteins could inhibit in vivo expression of delayed hypersensitivity during mycobacterial infections because of their interaction with T-cell FN.
Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells. (1994)

Tyrosine phosphorylation of cytoskeletal proteins occurs during integrin-mediated cell adhesion to extracellular matrix proteins. We have investigated the role of tyrosine phosphorylation in the migration and initial spreading of human umbilical vein endothelial cells (HUVEC). Elevated phosphotyrosine concentrations were noted in the focal adhesions of HUVEC migrating into wounds. Anti-phosphotyrosine Western blots of extracts of wounded HUVEC monolayers demonstrated increased phosphorylation at 120-130 kDa when compared with extracts of intact monolayers. The pp125FAK immunoprecipitated from wounded monolayers exhibited increased kinase activity as compared to pp125FAK from intact monolayers. The time to wound closure in HUVEC monolayers was doubled by tyrphostin AG 213 treatment. The same concentration of AG 213 interfered with HUVEC focal adhesion and stress fiber formation. AG 213 inhibited adhesion-associated tyrosine phosphorylation of pp125FAK in HUVEC. Tyrphostins AG 213 and AG 808 inhibited pp125FAK activity in in vitro kinase assays. pp125FAK immunoprecipitates from HUVEC treated with both of these inhibitors also had kinase activity in vitro that was below levels seen in untreated HUVEC. These findings suggest that tyrosine phosphorylation of cytoskeletal proteins may be important in HUVEC spreading and migration and that pp125FAK may mediate phosphotyrosine formation during these processes.
Pregnenolone esterification in Saccharomyces cerevisiae (1999)

While studying the effect of steroids on the growth of the yeast Saccharomyces cerevisiae, we found that pregnenolone was converted into the acetate ester. This reaction was identified as a transfer of the acetyl group from acetyl-CoA to the 3beta-hydroxyl group of pregnenolone. The corresponding enzyme, acetyl-CoA:pregnenolone acetyltransferase (APAT) is specific for Delta5- or Delta4-3beta-hydroxysteroids and short-chain acyl-CoAs. The apparent Km for pregnenolone is approximately 0.5 microm. The protein associated with APAT activity was partially purified and finally isolated from an SDS/polyacrylamide gel. Tryptic peptides were generated and N-terminally sequenced. Two peptide sequences allowed the identification of an open reading frame (YGR177c, in the S. cerevisiae genome database) translating into a 62-kDa protein of hitherto unknown function. This protein encoded by a gene known as ATF2 displays 37% identity with an alcohol acetyltransferase encoded by the yeast gene ATF1. Disruption of ATF2 led to the complete elimination of APAT activity and consequently abolished the esterification of pregnenolone. In addition, a toxic effect of pregnenolone linked to the disruption of ATF2 was observed. Pregnenolone toxicity is more pronounced when the atf2-Delta mutation is introduced in a yeast strain devoid of the ATP-binding cassette transporters, PDR5 and SNQ2. Our results suggest that Atf2p (APAT) plays an active role in the detoxification of 3beta-hydroxysteroids in association with the efflux pumps Pdr5p and Snq2p.
Intensity-based modified Doppler variance algorithm: application to phase instable and phase stable optical coherence tomography systems (2011)

The traditional phase-resolved Doppler method demonstrates great success for in-vivo imaging of blood flow and blood vessels. However, the phase-resolved method always requires high phase stability of the system. In phase instable situations, the performance of the phase-resolved methods will be degraded. We propose a modified Doppler variance algorithm that is based on the intensity or amplitude value. Performances of the proposed algorithm are compared with traditional phase-resolved Doppler variance and color Doppler methods for both phase stable and phase instable systems. For the phase instable situation, the proposed algorithm demonstrates images without phase instability induced artifacts. In-vivo imaging of window-chamber hamster skin is demonstrated for phase instable situation with a spectrometer-based Fourier domain OCT system. A microelectromechanical systems (MEMS) based swept source OCT (SSOCT) system is also used to demonstrate the performance of the proposed method in a phase instable situation. The phase stability of the SSOCT system is analyzed. In-vivo imaging of the blood vessel of human skin is demonstrated with the proposed method and the SSOCT system. For the phase stable situation, the proposed algorithm also demonstrates comparable performance with traditional phase-resolved methods. In-vivo imaging of the human choroidal blood vessel network is demonstrated with the proposed method under the phase stable situation. Depth-resolved fine choroidal blood vessel networks are shown.
Purification of Membrane-bound Lactoferrin from the Human Milk Fat Globule Membrane (2000)

Although lactoferrin is known as a basic soluble glycoprotein, the presence of the membrane-bound form of this protein has also been demonstrated in human milk. Membrane-bound lactoferrin was extracted from the human milk fat globule membrane with a detergent mixture of 1% Tween-20, 0.5% C12E8, and 0.5 M KCl in 20 mM Tris-HCl (pH 7.4). Lactoferrin in the detergent-soluble fraction was purified by affinity chromatography with Concanavalin A and by hydrophobic chromatography with phenyl-Superose. The purified protein gave a single band of 80 kDa by SDS-PAGE. Its N-terminal amino acid sequence was consistent with that of human lactoferrin.
OX40 (CD134) Controls Memory T Helper 2 Cells that Drive Lung Inflammation (2003)

Asthma is caused by memory Th2 cells that often arise early in life and persist after repeated encounters with allergen. Although much is known regarding how Th2 cells develop, there is little information about the molecules that regulate memory Th2 cells after they have formed. Here we show that the costimulatory molecule OX40 is expressed on memory CD4 cells. In already sensitized animals, blocking OX40-OX40L interactions at the time of inhalation of aerosolized antigen suppressed memory effector accumulation in lung draining lymph nodes and lung, and prevented eosinophilia, airway hyperreactivity, mucus secretion, and Th2 cyto-kine production. Demonstrating that OX40 signals directly regulate memory T cells, antigen-experienced OX40-deficient T cells were found to divide initially but could not survive and accumulate in large numbers after antigen rechallenge. Thus, OX40-OX40L interactions are pivotal to the efficiency of recall responses regulated by memory Th2 cells.
Implementation of an optical crossbar network based on directional switches (1992)

We have implemented an all-optical digital switching network based on ferroelectric liquid crystal arrays used as directional switches. It connects eight input to eight output channels simultaneously and randomly. The reconfiguration rate of the system can reach 100 kHz by using a parallel-driven 6 x 6 ferroelectric liquid crystal array. The modulation bandwidth in each channel depends only on the transmitters and receivers, not on the system itself.
Outcome of Congestive Heart Failure in Elderly Persons: Influence of Left Ventricular Systolic Function: The Cardiovascular Health Study (2002)

Community-dwelling elderly persons, especially those with impaired left ventricular function, have a substantial risk for death from congestive heart failure. However, more deaths occur from heart failure in persons with normal systolic function because left ventricular function is more often normal than impaired in elderly persons with heart failure.
Classroom Testing Procedures and Student Anxiety (1983)

An attempt to reduce test anxiety was made by pro viding increased information to students a out the types of questions likely to appear on end-of-unit ex aminations. Two experimental testing formats, and a combination of the two, were compared with the typical practice of providing no supplemental information. The experimental formats were as follows: (1) During the class session prior to the test, a copy of a previous test, given to a class in an earlier semester but covering the same content, was given to each student. Students were told that similar, but not identical, questions could be expected on the upcoming test. (2) Students were of fered two opportunities to be tested over the same course unit at consecutive class sessions. They were re quired to take one, although they could take both, of the tests. Their score of record would be the better of the two scores on the tests. The tests contained com pletely different sets of questions, but did cover the same unit of study. Table 1 summarizes the testing design as it was employed over a full year's classes in Introductory Sociology. Each testing condition was used at least three times during the year. For example, the combination of experimental formats, (t)TT, was employed with Unit 1 for both of the Fall 1981 classes, with Unit 2 for the
Reversed and Sandwiched Analogs of Duocarmycin SA:  Establishment of the Origin of the Sequence-Selective Alkylation of DNA and New Insights into the Source of Catalysis (1997)

The synthesis and examination of two unique classes of duocarmycin SA analogs are described which we refer to as reversed and sandwiched analogs. Their examination established both the origin of the DNA alkylation selectivity and that both enantiomers of this class of natural products are subject to the same polynucleotide recognition features. The most beautiful demonstration of this is the complete switch in the enantiomeric alkylation selectivity of the reversed analogs which is only consistent with the noncovalent binding model and incompatible with alkylation site models of the origin of the DNA alkylation selectivity. In addition, dramatic alterations in the rates of DNA alkylation were observed among the agents and correlate with the presence or absence of an extended, rigid N2 amide substituent. This has led to the proposal of a previously unrecognized source of catalysis for the DNA alkylation reaction which was introduced in the preceding paper of this issue (J. Am. Chem. Soc. 1997, 119, 4977−4986).
Measurement of Interhelical Electrostatic Interactions in the GCN4 Leucine Zipper (1995)

The dimerization specificity of the bZIP transcription factors resides in the leucine zipper region. It is commonly assumed that electrostatic interactions between oppositely charged amino acid residues on different helices of the leucine zipper contribute favorably to dimerization specificity. Crystal structures of the GCN4 leucine zipper contain interhelical salt bridges between Glu20 and Lys15' and between Glu22 and Lys27'. 13C-nuclear magnetic resonance measurements of the glutamic acid pKa values at physiological ionic strength indicate that the salt bridge involving Glu22 does not contribute to stability and that the salt bridge involving Glu20 is unfavorable, relative to the corresponding situation with a neutral (protonated) Glu residue. Moreover, the substitution of Glu20 by glutamine is stabilizing. Thus, salt bridges will not necessarily contribute favorably to bZIP dimerization specificity and may indeed be unfavorable, relative to alternative neutral-charge interactions.
HUMAN MONOCLONAL ANTIBODIES (1984)

Journal Article HUMAN MONOCLONAL ANTIBODIES Get access K SIKORA, MRCP FRCR PhD K SIKORA, MRCP FRCR PhD Ludwig Institute for Cancer Research MRC CentreCambridge Search for other works by this author on: Oxford Academic PubMed Google Scholar British Medical Bulletin, Volume 40, Issue 3, 1984, Pages 209–212, https://doi.org/10.1093/oxfordjournals.bmb.a071978 Published: 01 September 1984
Basic principles of organic chemistry (1965)

Basic principles of organic chemistry , Basic principles of organic chemistry , کتابخانه مرکزی دانشگاه علوم پزشکی تهران
The use of solid-supported reagents for the multi-step synthesis of analytically pure α,β-unsaturated compounds in miniaturized flow reactors (2007)

Micro reaction technology offers a safe, controllable and information rich technique suitable for the long-term production of pharmaceutical agents and fine chemicals. To date however, few of the syntheses performed using this technology have addressed the problems associated with product purification. With this in mind, we report herein the incorporation of multiple supported reagents into EOF-based miniaturized flow reactors for the two-step synthesis of analytically pure compounds. Using this approach, the successful synthesis of 20 alpha,beta-unsaturated compounds in excellent yields (>99.1%) and purities (>99.9%) has been achieved, illustrating significant improvements compared to traditional batch techniques.
Targeting of perfluorocarbon microbubbles to selective populations of circulating blood cells (2009)

Perfluorocarbon microbubbles (MBs) are routinely used as in vivo ultrasound contrast reagent. In addition, there is great interest in the use of MBs for ultrasound-mediated delivery, for example, of drugs and genes. As MB size (1-10 microm) limits their distribution to the vasculature, we aimed to assess the efficiency of targeting MBs to circulating cells using antibodies. Our experiments showed that MBs efficiently bind to erythrocytes and B-lymphoma cells in blood. The maximum binding was reached at a cell/MB ratio of 1:1. Following binding, the cells acquired buoyancy that allowed their easy separation from blood with brief centrifugation. Coating the MBs with DNA did not interfere with binding to cells. Experiments in mice showed that intravenously injected targeted MBs efficiently chased and bound to preinjected target cells in the bloodstream. Our data demonstrate the potential in targeting of blood cells for diagnostics and therapy.
Track height reduction for standard-cell in below 5nm node: how low can you go? (2018)

The targeted 5nm and below technology node at IMEC has been defined by poly pitch 42nm and metal pitch 21nm. Compared to the previous node the CPP [1] remains the same and only the metal pitch is scaled down, which implies that direct pitch scaling will not lead to the most optimum scaling. Therefore, Standard Cell (SDC) track height reduction is a knob that can be used to achieve advances in the scaling of the technology to preserve Moore’s law. Here we present some of the options for the standard cell design that may enable this advance technology node and will require scaling boosters as Design-Technology co-optimization (DTCO).
Nucleotide sequences of eight human natural autoantibody VH regions reveals apparent restricted use of VH families. (1989)

Eight full length cDNA were isolated from EBV transformed human PBL derived from different normal individuals. Five were derived from antibodies with the characteristics of natural polyreactive antibodies. Three were either monoreactive or bireactive. The most striking feature of the structure of these molecules was their utilization of VH families. Although three used the large VHIII family and one used the large VHI family, the other four used genes derived from two of the recently defined small human VH families VHIV and VHV. Three of the molecules represent VHIV expressed sequences and one is the first example of a VHV gene used in an antibody of defined specificity. The nucleotide sequences of some of the molecules were remarkably similar in their VH gene segments to previously described VH genes. The data suggest that natural autoantibodies may use a restricted portion of the VH repertoire, and, in addition, that some polyreactive antibodies may be germ line encoded. The implication of these findings for the origin and diversity of the human B cell repertoire is discussed.