gebhart/patrepeval huggingface dataset:
Axial profile of grating coupled radiation from second-order DFB lasers with phase shifts (1990)

Axial profiles of the grating-coupled radiation field emitted in a direction normal to the surface of distributed-feedback (DFB) lasers with phase-shifted second-order corrugation are theoretically analyzed. The profiles are calculated for nonreflecting DFB lasers with the following phase shifts: λ/4 shift, two lambda /8 shifts, and two 3 lambda /8 shifts. It is demonstrated that the radiation field can be controlled by changing the phase shifts. This result suggests a new method for modulating the surface emission of a DFB laser.
Purification and characterization of a newly identified growth factor specific for epithelial cells. (1989)

A growth factor specific for epithelial cells was identified in conditioned medium of a human embryonic lung fibroblast cell line. The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of ultrafiltration, heparin-Sepharose affinity chromatography, and hydrophobic chromatography on a C4 reversed-phase HPLC column. KGF was both acid and heat labile and consisted of a single polypeptide chain of approximately 28 kDa. Purified KGF was a potent mitogen for epithelial cells, capable of stimulating DNA synthesis in quiescent BALB/MK epidermal keratinocytes by greater than 500-fold with activity detectable at 0.1 nM and maximal at 1.0 nM. Lack of mitogenic activity on either fibroblasts or endothelial cells indicated that KGF possessed a target cell specificity distinct from any previously characterized growth factor. Microsequencing revealed an amino-terminal sequence containing no significant homology to any known protein. The release of this growth factor by human embryonic fibroblasts raises the possibility that KGF may play a role in mesenchymal stimulation of normal epithelial cell proliferation.
Disposable Smart Lab on a Chip for Point-of-Care Clinical Diagnostics (2004)

This paper presents the development of a disposable plastic biochip incorporating smart passive microfluidics with embedded on-chip power sources and integrated biosensor array for applications in clinical diagnostics and point-of-care testing. The fully integrated disposable biochip is capable of precise volume control with smart microfluidic manipulation without costly on-chip microfluidic components. The biochip has a unique power source using on-chip pressurized air reservoirs, for microfluidic manipulation, avoiding the need for complex microfluidic pumps. In addition, the disposable plastic biochip has successfully been tested for the measurements of partial oxygen concentration, glucose, and lactate level in human blood using an integrated biosensor array. This paper presents details of the smart passive microfluidic system, the on-chip power source, and the biosensor array together with a detailed discussion of the plastic micromachining techniques used for chip fabrication. A handheld analyzer capable of multiparameter detection of clinically relevant parameters has also been developed to detect the signals from the cartridge type disposable biochip. The handheld analyzer developed in this work is currently the smallest analyzer capable of multiparameter detection for point-of-care testing.
Attractive Interhelical Electrostatic Interactions in the Proline- and Acidic-rich Region (PAR) Leucine Zipper Subfamily Preclude Heterodimerization with Other Basic Leucine Zipper Subfamilies (2000)

Basic region-leucine zipper (B-ZIP) proteins homo- or heterodimerize to bind sequence-specific double-stranded DNA. We present circular dichroism (CD) thermal denaturation data on vitellogenin promoter-binding protein (VBP), a member of the PAR subfamily of B-ZIP proteins that also includes thyroid embryonic factor, hepatocyte leukemia factor, and albumin site D-binding protein. VBP does not heterodimerize with B-ZIP domains from C/EBP alpha, JUND, or FOS. We describe a dominant negative protein, A-VBP, that contains the VBP leucine zipper and an acidic amphipathic protein sequence that replaces the basic region critical for DNA binding. The acidic extension forms a coiled coil structure with the VBP basic region in the VBP.A-VBP heterodimer. This new alpha-helical structure extends the leucine zipper N-terminally, stabilizing the complex by 2.0 kcal/mol. A-VBP abolishes DNA binding of VBP in an equimolar competition assay, but does not affect DNA binding even at 100-fold excess of CREB, C/EBP alpha, or FOS/JUND. Likewise, proteins containing the acidic extension appended to seven other leucine zippers do not inhibit VBP DNA binding. We show that conserved g <--> e' or i, i' +5 salt bridges are sufficient to confer specificity to VBP by mutating the C/EBPalpha leucine zipper to contain the g <--> e' salt bridges that characterize VBP. A-VBP heterodimerizes with this mutant C/EBP, preventing it from binding to DNA. These conserved g <--> e' electrostatic interactions define the specificity of the PAR subfamily of B-ZIP proteins and preclude interaction with other B-ZIP subfamilies.
High-speed performance of OMCVD grown InAlAs/InGaAs MSM photodetectors at 1.5 mu m and 1.3 mu m wavelengths (1989)

A report is presented on the fabrication of high-speed In/sub 0.53/Ga/sub 0.47/As metal-semiconductor-metal (MSM) photodetectors incorporating a high-quality lattice-matched InAlAs barrier enhancement layer, grown by organometallic chemical vapor deposition (OMCVD). Fast responses of approximately 55 ps full-width half-maximum at 1.5 mu m and approximately 48 ps at 1.3 mu m wavelengths are observed, corresponding to intrinsic device bandwidths of approximately 8 GHz and approximately 11 GHz, respectively. The absence of any tail to the pulse response, and of any low-bias DC gain, indicates a low-trap density at the InAlAs/InGaAs heterointerface. Bias independent dark currents of 10-20 mu A are observed below breakdown, which occurred at >30 V in devices with a 500-A-thick InAlAs layer.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in Campylobacter jejuni (1986)

Three human isolates of Campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. Cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. Increased numbers of longer, more filamentous forms were seen in Gram-stained smears. Three proteins, with apparent Mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. At least one of these proteins (76,000 to 74,000) was exposed on the cell surface. A bioassay was used to look for the production of siderophores by these and other strains of C. jejuni. Seven of 26 strains tested produced detectable amounts of siderophores. Growing strains at 42 degrees C failed to suppress siderophore synthesis or to alter the outer membrane protein profiles of iron-starved cells. The ability of three strains to utilize exogenously supplied siderophores for growth in low-iron medium was also examined. All three strains were able to utilize enterochelin and ferrichrome, but none utilized aerobactin, rhodotorulic acid, or desferrioxamine B. The effect of iron on the virulence of C. jejuni for 11-day-old chicken embryos inoculated via the chorioallantoic membrane was also determined.
Common Forms of Synaptic Plasticity in the Hippocampus and Neocortex in Vitro (1993)

Activity-dependent synaptic plasticity in the superficial layers of juvenile cat and adult rat visual neocortex was compared with that in adult rat hippocampal field CA1. Stimulation of neocortical layer IV reliably induced synaptic long-term potentiation (LTP) and long-term depression (LTD) in layer III with precisely the same types of stimulation protocols that were effective in CA1. Neocortical LTP and LTD were specific to the conditioned pathway and, as in the hippocampus, were dependent on activation of N-methyl-D-aspartate receptors. These results provide strong support for the view that common principles may govern experience-dependent synaptic plasticity in CA1 and throughout the superficial layers of the mammalian neocortex.
3DAS: a 3-dimensional-structure authentication system (1995)

It has been argued that the degree of microstructural order of an optical security feature is an approximate measure of its practical value for document security. Examples of highly ordered structures are diffractive devices like holograms, kinograms and zero order devices as well as interference devices like multilayer thin films and liquid crystals. An entirely different type of security feature is the random device which combines a complete lack of structural order with a high degree of security. This paper surveys the polarity of ordered and random structures.
Inside risksReflections on Conficker (2009)

An insider's view of the analysis and implications of the Conficker conundrum.
Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging (2011)

Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.
An Improved Relative GNSS Tracking Method Utilizing Single Frequency Receivers (2020)

The Global Navigation Satellite Systems (GNSS) becomes the primary choice for device localization in outdoor situations. At the same time, many applications do not require precise absolute Earth coordinates, but instead, inferring the geometric configuration information of the constituent nodes in the system by relative positioning. The Real-Time Kinematic (RTK) technique shows its efficiency and accuracy in calculating the relative position. However, when the cycle slips occur, the RTK method may take a long time to obtain a fixed ambiguity value, and the positioning result will be a "float" solution with a low meter accuracy. The novel method presented in this paper is based on the Relative GNSS Tracking Algorithm (Regtrack). It calculates the changes in the relative baseline between two receivers without an ambiguity estimation. The dead reckoning method is used to give out the relative baseline solution while a parallel running Extended Kalman Filter (EKF) method reinitiates the relative baseline when too many validation failures happen. We conducted both static and kinematic tests to assess the performance of the new methodology. The experimental results show that the proposed strategy can give accurate millimeter-scale solutions of relative motion vectors in adjacent two epochs. The relative baseline solution can be sub-decimeter level with or without the base station is holding static. In the meantime, when the initial tracking point and base station coordinates are precisely obtained, the tracking result error can be only 40 cm away from the ground truth after a 25 min drive test in an urban environment. The efficiency test shows that the proposed method can be a real-time method, the time that calculates one epoch of measurement data is no more than 80 ms and is less than 10 ms for best results. The novel method can be used as a more robust and accurate ambiguity free tracking approach for outdoor applications.
Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen (1994)

Cloning of a cDNA from Cry j II, the second major allergen from Japanese cedar (Cryptomeria japonica) pollen, is described. An isolated Cry j II cDNA contained an open reading frame coding for 514 amino acid residues. The mature Cry j II protein consisted of 388 amino acid residues (R46-S433). According to a homology analysis, no amino acid sequence homology was observed between Cry j II and Cry j I, another major allergen. But Cry j II showed homology with polygalacturonase (PG) derived from tomato (40% identity) at the amino acid level. The sequence information can potentially be used to devise an effective course of immunotherapy for Japanese cedar pollinosis.
The Complete E-Commerce Book:Design, Build & Maintain a Successful Web-based Business (2000)

From the Publisher:
Make your e-commerce vision a success with this comprehensive, step-by-step handbook. Whether your company is a startup or well-established, you'll learn how to plan, implement and operate a successful e-commerce site - from selecting the right software through fulfilling orders.
The expert author starts by helping you plan the customer experience and design an effective, easy access interface. You then progress to cutting-edge programming techniques and robust server configuration. Next, you'll master site marketing, customer service, order processing, warehousing and shipping. Along the way are plenty of real-world examples of Web sites to explore so you can learn what to do - and what not to do. Using this book as a tutorial and a reference, you'll learn how to: 
Create a custom business model for success. Select the software, hardware and hosting service that will best meet your needs. Master outsourcing, building traffic, order processing and fulfillment. Choose the type of site that will fit your needs by reviewing current examples of brochureware sites, online stores, subscriptions sites, cyber malls and more. Choose the right vendors and consultants to get the job done - and learn how to manage their work effectively. Build a web site that stands out form the rest. Keep your customers happy with easy access, prompt delivery, good customer service and continuous enhancement of site quality and content.
Business owners, Web entrepreneurs and everyone who's involved with setting up and rolling out an e-commerce site will find answers to their business and technical questions in this all-in-one handbook!
Porcine gastric mucin binds to recombinant norovirus particles and competitively inhibits their binding to histo-blood group antigens and Caco-2 cells (2005)

This is the first report of the binding of glycoproteins from animal gastric mucosa to human NVs. This study highlights the importance of further characterizing the NV incidence and infections in nonhuman animal hosts and the possibility that NV is a zoonotic disease.
Differential regulation of T cell antigen responsiveness by isoforms of the src-related tyrosine protein kinase p59fyn. (1992)

Recent observations suggest that the src-related tyrosine protein kinase p59fyn may be involved in antigen-induced T lymphocyte activation. As a result of alternative splicing, p59fyn exists as two isoforms that differ exclusively within a short sequence spanning the end of the Src Homology 2 (SH2) region and the beginning of the tyrosine protein kinase domain. While one p59fyn isoform (fynB) is highly expressed in brain, the alternative product (fynT) is principally found in T lymphocytes. To further understand the role of p59fyn in T cell activation and to test the hypothesis that p59fynT serves a tissue-specific function in T lymphocytes, we have examined the effects of expression of activated versions (tyrosine 528 to phenylalanine 528 mutants) of either form of p59fyn on the physiology of an antigen-specific mouse T cell hybridoma. Our results demonstrated that the two forms of fyn, expressed in equivalent amounts, efficiently enhanced antibody-induced T cell receptor (TCR)-mediated signals. In contrast, only p59fynT increased interleukin 2 production in response to antigen stimulation. This finding implies that the distinct p59fyn isoform expressed in T lymphocytes regulates the coupling of TCR stimulation by antigen/major histocompatibility complex to lymphokine production.
Terrain perception for DEMO III (2002)

The Demo III program has as its primary focus the development of autonomous mobility for a small rugged cross country vehicle. Enabling vision based terrain perception technology for classification of scene geometry and material is currently under development at JPL. In this paper we report recent progress on both stereo-based obstacle detection and terrain cover color-based classification. Our experiments show that the integration of geometric description and terrain cover characterization may be the key to enabling successful autonomous navigation in cross-country vegetated terrain.
Cilk: An Efficient Multithreaded Runtime System (1996)

Cilk (pronounced “silk”) is a C-based runtime system for multithreaded parallel programming. In this paper, we document the efficiency of the Cilk work-stealing scheduler, both empirically and analytically. We show that on real and synthetic applications, the “work” and “critical-path length” of a Cilk computation can be used to model performance accurately. Consequently, a Cilk programmer can focus on reducing the computation's work and critical-path length, insulated from load balancing and other runtime scheduling issues. We also prove that for the class of “fully strict” (well-structured) programs, the Cilk scheduler achieves space, time, and communication bounds all within a constant factor of optimal. The Cilk runtime system currently runs on the Connection Machine CM5 MPP, the Intel Paragon MPP, the Sun Sparcstation SMP, and the Cilk-NOW network of workstations. Applications written in Cilk include protein folding, graphic rendering, backtrack search, and the ★Socrates chess program, which won second prize in the 1995 ICCA World Computer Chess Championship.
New developments in high-throughput resequencing and variation detection using high density microarrays (2002)

We developed a high-throughput method for resequencing for single nucleotide polymorphism (SNP) discovery using high-density microarrays. Over the two-year course of this study a number of improvements in sample preparation methods, hybridization assay, array handling, and analysis method were developed and implemented. DNA from 40 unrelated individuals of three different ethnic origins was amplified, labeled, and hybridized to arrays designed with probes representing genomic, coding, and regulatory regions. Protocol improvements including the use of long PCR and semi-automation reduced labeling and fragmentation costs by 33%. Automation improvements include the development of a scanner autoloader for arrays, a faster array wash station, and a linked laboratory tracking and data management system. Validation of a smaller feature size, 20 x 24 microns, allowed the simultaneous screening of 30-kb sense and 30-kb antisense DNA on each microarray, increasing throughput to 1.4 Mb per day per two laboratory personnel. More than 15,000 SNPs were identified in 8.3 Mb of the human genome using high-density resequencing and variation detection arrays (microarrays).
Privacy Preserving Data Classification with Rotation Perturbation (2006)

Data perturbation techniques are one of the most popular models for privacy preserving data mining (Agrawal and Srikant, 2000; Aggarwal and Yu, 2004). It is especially convenient for applications where the data owners need to export/publish the privacy-sensitive data. A data perturbation procedure can be simply described as follows. Before the data owner publishes the data, they randomly change the data in certain way to disguise the sensitive information while preserving the particular data property that is critical for building the data models. Several perturbation techniques have been proposed recently, among which the most typical ones are randomization approach (Agrawal and Srikant, 2000) and condensation approach (Aggarwal and Yu, 2004).
Palladium‐Catalyzed Intermolecular Alkenylation of Indoles by Solvent‐Controlled Regioselective CH Functionalization (2005)

Either the C2- or the C3-substituted product can be obtained with the same palladium(II) catalyst in an oxidative intermolecular alkenylation of indoles. A variety of conditions can be used for derivatization at the 3-position; however, the presence of acetic acid is required for the C2-selective process (see scheme). Further elaboration of the products by a similar CH functionalization process leads to the bisalkenylated indoles selectively.
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure (1995)

An improved lithium acetate (LiAc)/single-stranded DNA (SS-DNA)/polyethylene glycol (PEG) protocol which yields > 1 x 10(6) transformants/micrograms plasmid DNA and the original protocol described by Schiestl and Gietz (1989) were used to investigate aspects of the mechanism of LiAc/SS-DNA/PEG transformation. The highest transformation efficiency was observed when 1 x 10(8) cells were transformed with 100 ng plasmid DNA in the presence of 50 micrograms SS carrier DNA. The yield of transformants increased linearly up to 5 micrograms plasmid per transformation. A 20-min heat shock at 42 degrees C was necessary for maximal yields. PEG was found to deposit both carrier DNA and plasmid DNA onto cells. SS carrier DNA bound more effectively to the cells and caused tighter binding of 32P-labelled plasmid DNA than did double-stranded (DS) carrier. The LiAc/SS-DNA/PEG transformation method did not result in cell fusion. DS carrier DNA competed with DS vector DNA in the transformation reaction. SS plasmid DNA transformed cells poorly in combination with both SS and DS carrier DNA. The LiAc/SS-DNA/PEG method was shown to be more effective than other treatments known to make cells transformable. A model for the mechanism of transformation by the LiAc/SS-DNA/PEG method is discussed.
The Effect of Hippotherapy on Functional Outcomes for Children with Disabilities: A Pilot Study (2008)

This study represents an initial attempt to use the GAS in a single-case design with a variety of pediatric diagnoses.
Comprehensive Assessment of Metabolic Enzyme and Transporter Genes Using the Affymetrix ® Targeted Genotyping System (2007)

The combined effects of multiple polymorphisms in several drug-metabolizing enzyme and transporter genes can contribute to considerable interindividual variation in drug disposition and response. Therefore, it has been of increasing interest to generate scalable, flexible and cost-effective technologies for large-scale genotyping of the drug-metabolizing enzyme and transporter genes. However, the number of drug-metabolizing enzyme and transporter gene variants exceeds the capacity of current technologies to comprehensively assess multiple polymorphisms in a single, multiplexed assay. The Targeted Genotyping System (Affymetrix, CA, USA) provides a solution to this challenge, by combining molecular inversion probe technology with universal microarrays to provide a method that is capable of analyzing thousands of variants in a single reaction, while remaining relatively insensitive to cross-reactivity between reaction components. This review will focus on the Targeted Genotyping System and how this technology was adapted to enable comprehensive analysis of drug-metabolizing enzyme and transporter gene polymorphisms.