gebhart/patrepeval huggingface dataset:
Opposite Regulation of Gax Homeobox Expression by Angiotensin II and C-Type Natriuretic Peptide (1997)

Growth arrest-specific homeobox (Gax) gene was isolated from rat aorta cDNA library and its expression was largely confined to the cardiovascular tissues. Gax gene was rapidly downregulated by platelet-derived growth factor in vascular smooth muscle cells (VSMCs) and overexpressed Gax was reported to reduce the neointimal thickening after balloon injury in vivo. We have demonstrated that angiotensin II (Ang II) stimulates vascular growth. In contrast, we also reported that C-type natriuretic peptide (CNP) is secreted from vascular endothelial cells to act as a novel endothelium-derived relaxing peptide and inhibits vascular growth via cGMP cascade. In the present study, we examined the effects of Ang II and CNP on Gax gene expression in VSMCs. In quiescent rat aortic VSMCs. Gax mRNA (2 3 kb) level became negligible 6 hours after the addition of Ang II (10(-6) mol/L). The inhibitory action of Ang II on Gax mRNA expression (ED50: 10(-11) mol/L) was almost completely blocked by an AT1R antagonist, CV11974. In contrast, CNP 10(-6) mol/L augmented Gax mRNA expression to exhibit 1.8-fold increase of the control 12 hours after the stimulation. This effect of CNP was mimicked by the addition of 8-bromoadenosine 3'-5'-cyclic monophosphate. The addition of C-ANF[4-23], an atrial natriuretic peptide-C receptor-specific agonist and devoid of stimulating cGMP production, exhibited no effect on Gax mRNA expression. Simultaneous administration of Ang II and CNP revealed that CNP (10(-6) mol/L) significantly attenuated the inhibitory action of Ang II (10(-10) mol/L) on Gax mRNA expression. These results suggest that Gax is a common transcription factor involved in the signaling pathway of vascular growth for Ang II and CNP and regulates the cell cycle and/or phenotype of VSMCs for vascular remodeling in hypertension and atherosclerosis.
Photocrosslinkable Polythiophenes for Efficient, Thermally Stable, Organic Photovoltaics (2009)

Abstract Photocrosslinkable bromine‐functionalized poly(3‐hexylthiophene) (P3HT‐Br) copolymers designed for application in solution‐processed organic photovoltaics are prepared by copolymerization of 2‐bromo‐3‐(6‐bromohexyl) thiophene and 2‐bromo‐3‐hexylthiophene. The monomer ratio is carefully controlled to achieve a UV photocrosslinkable layer while retaining the π–π stacking feature of the conjugated polymers. The new materials are used as electron donors in both bulk heterojunction (BHJ) and bilayer type photovoltaic devices. Unlike devices prepared from either P3HT:PCBM blend or P3HT‐Br:PCBM blend without UV treatment, photocrosslinked P3HT‐Br:PCBM devices are stable even when annealed for two days at the elevated temperature of 150 °C as the nanophase separated morphology of the bulk heterojunction is stabilized as confirmed by optical microscopy and grazing incidence wide angle X‐ray scattering (GIWAXS). When applied to solution‐processed bilayer devices, the photocrosslinkable materials show high power conversion efficiencies (∼2%) and excellent thermal stability (3 days at 150 °C). Such performance, one of the highest obtained for a bilayer device fabricated by solution processing, is achieved as crosslinking does not disturb the π–π stacking of the polymer as confirmed by GIWAXS measurements. These novel photocrosslinkable materials provide ready access to efficient bilayer devices thus enabling the fundamental study of photophysical characteristics, charge generation, and transport across a well‐defined interface.
Providing audible information relating to the differences in monophonic and multichannel sound reinforcement systems using computer auralization (2003)

When selecting the sound distribution system for a particular venue, there is often a decision to be made as to the audible differences between a monophonic and potentially less expensive loudspeaker system and a multi-channel and potentially more expensive system. Computer auralization is a tool that can be used for auditioning the approximate differences between single channel and multi-channel loudspeaker systems.
Clinical significance of MCP‐1 levels in BALF and serum in patients with interstitial lung diseases (1999)

It has previously been reported that the expression of monocyte chemoattractant protein-1 (MCP-1) in the lung tissues of patients with idiopathic pulmonary fibrosis (IPF) was different from that in the tissues of patients with other interstitial lung diseases (ILDs). The aim of this study was to determine whether this difference reflects the amount of MCP-1 in the bronchoalveolar lavage fluid (BALF) or serum of patients with ILD, and whether such a correlation, if it exists, is clinically useful. MCP-1 concentrations in the BALF and sera were evaluated in 86 patients with ILDs including IPF, acute interstitial pneumonia, interstitial pneumonia with collagen vascular disease (IP-CVD), chronic interstitial pneumonia (CIP), bronchiolitis obliterans-organizing pneumonia, sarcoidosis, hypersensitivity pneumonitis, and in 10 normal healthy volunteers who were controls (NC). BALF MCP-1 levels were significantly elevated in the IPF, IP-CVD, CIP and sarcoidosis groups compared with the NC group. The level in the IPF group was significantly higher than that in any other patient group. Serum MCP-1 levels in the IPF, IP-CVD, CIP and sarcoidosis groups were significantly higher than the NC group. No statistical difference was found in serum MCP-1 levels between the IPF, IP-CVD and CIP groups. BALF MCP-1 levels were significantly higher than serum MCP-1 levels in the IPF group and lower than in the IP-CVD and CIP groups. Serum MCP-1 levels correlated with the clinical course of ILD treated with corticosteroid therapy. These results show that measurement of monocyte chemoattractant protein-1 levels in both bronchoalveolar lavage fluid and serum may be helpful in discriminating idiopathic pulmonary fibrosis from other types of interstitial lung disease and that monitoring of serum monocyte chemoattractant protein-1 may be useful for predicting the clinical course of interstitial lung diseases.
Gasification of Pyrocatechol in Supercritical Water in the Presence of Potassium Hydroxide (2000)

The results presented are fundamental investigations with regard to developing a process for hydrogen production from high moisture biomass and wastewater. Pyrocatechol is used as a model compound for lignin in biomass and for aromatic compounds in wastewaters. The experiments of pyrocatechol gasification provides knowledge of the chemical reactions occurring during gasification and their dependence on reaction conditions. The experiments were carried out in two different reactor types, a batch and a tubular reactor, to achieve long reaction times at low temperatures as well as short reaction times at high temperatures. The pressure, temperature, concentration, and reaction time dependence of the gasification is presented and compared with calculated equilibrium data. The influence of potassium hydroxide addition to improve the hydrogen yield was studied and the effect of different catalysts is discussed.
[Type B intramural hematoma rupture]. (2007)

A 70-year-old male, who had complained sudden chest pain and sweating, was admitted to our hospital and diagnosed as a type B intramural hematoma rupture. Emergency repair was performed with arch replacement and open-stent method. Post-operative recovery was satisfactory and the patient was discharged. A type B intramural hematoma rupture is relatively rare. Trans-esophagial echocardiography is quite useful to detect the aortic lesion and to determine the ideal depth of the stent-graft.
Comparison of Cytokine Effects on Mouse Pancreatic α-Cell and β-Cell Lines Viability, Secretory Function, and MHC Antigen Expression (1990)

Cytokine effects on permanent cell lines of transformed mouse pancreatic alpha- and beta-cells were compared. The beta-tumor cell 1 (beta TC1) line (from an adenoma created in transgenic mice expressing the SV40 large T-antigen oncogene under control of the rat insulin II promoter) produced insulin predominantly, although small quantities of intracellular glucagon (100:1 insulin to glucagon) were detectable by radioimmunoassay. The alpha TC1 line (from an adenoma created in transgenic mice expressing the SV40 large T-antigen oncogene under control of the rat preproglucagon promoter) produced not only glucagon but also considerable quantities of insulin (4:1 glucagon to insulin) and preproinsulin mRNA. We therefore cloned alpha TC1 cells and obtained 12 glucagon-producing clonal cell lines that did not produce levels of insulin detectable by radioimmunoassay. Analysis by Northern blotting of total RNA from two lines, alpha TC1 clones 6 and 9, confirmed the absence of preproinsulin mRNA. No somatostatin or pancreatic polypeptide was detected by immunohistochemical staining in alpha TC1 clones 6 or 9 or beta TC1 cells. Rat recombinant gamma-interferon (IFN-gamma; 5-250 U/ml) or mouse recombinant interleukin 1 (IL-1; 1-25 U/ml) individually inhibited DNA synthesis in beta TC1 cells after 3 days of treatment. The two cytokines in combination acted synergistically to further depress DNA synthesis and increase cytotoxicity. In contrast, alpha TC1 clone 9 cells were not sensitive to inhibition of DNA synthesis by each cytokine individually, although glucagon synthesis was inhibited. The combination of these cytokines caused marked inhibition of DNA and glucagon syntheses in alpha TC1 clone 9 cells. alpha TC1 clone 9 cells were somewhat more resistant to the cytotoxic action of the combined cytokines than were beta TC1 cells. Incubation with 50 U/ml IFN-gamma induced class II MHC molecules (I-Ab, I-Ad, and I-Ed) and enhanced the constitutive expression of class I molecules (H-2Kb and H-2Kd) on the cell surfaces of beta TC1, uncloned alpha TC1, and alpha TC1 clones 6 and 9. Thus, these cell lines are heterozygous for MHC alleles derived from both parental strains used in the construction of the transgenic mice [C57BL/6J (H-2b) and DBA/2J (H-2d)]. Class II gene transcription induced by IFN-gamma was confirmed in beta TC1 and alpha TC1 clone 9 cells by Northern blot analysis with A alpha-, A beta-, E alpha, and E beta-DNA probes.(ABSTRACT TRUNCATED AT 400 WORDS)
Microwave Irradiation Effect on the Dispersion and Thermal Stability of RGO Nanosheets within a Polystyrene Matrix (2014)

Polystyrene-reduced graphene oxide (PSTY/RGO) composites were prepared via the <i>in situ</i> bulk polymerization method using two different preparation techniques. The general approach is to use microwave irradiation (MWI) to enhance the exfoliation and the dispersion of RGO nanosheets within the PSTY matrix. In the first approach, a mixture of GO and styrene monomers (STY) were polymerized using a bulk polymerization method facilitated by microwave irradiation (MWI) to obtain R-(GO-PSTY) composites. In the second approach, a mixture of RGO and STY monomers were polymerized using a bulk polymerization method to obtain RGO-(PSTY) composites. The two composites were characterized by FTIR, ¹H-NMR, XRD, SEM, HRTEM, TGA and DSC. The results indicate that the composite obtained using the first approach, which involved MWI, had a better morphology and dispersion with enhanced thermal stability, compared with the composites prepared without MWI. Moreover, DSC results showed that the <i>T</i><sub>g</sub> value of the composites after loading the RGO significantly increased by 24.6 °C compared to the neat polystyrene.
InGaP/InGaAs/Ge high concentration solar cell development at Emcore (2005)

Results from the Emcore triple junction (3J) terrestrial concentrator cell development program are presented. The cell is intended for use at concentrations equal to or exceeding 500/spl times/ geometrical. Leveraging our 3J space cell we have redesigned the emitter/grid structure, the tunnel diode interconnects, and top cell base thickness for use under concentrated terrestrial illumination. We have achieved a conversion efficiency of 32.8% (low AOD spectrum) at 165/spl times/. Methods for achieving still higher efficiencies are discussed, including the development of a metamorphic 3J cell. We have grown and characterized InGaAs metamorphic materials on Ge to be used as virtual substrates in a metamorphic 3J cell. The metamorphic InGaAs material has photoluminescence decay times characteristic of nearly radiatively limited material.
Evaluation of the Protective Potency of New Tuberculosis Vaccines (1989)

Animal models used to evaluate the relative protective potency of a panel of tuberculosis vaccines have yielded dissimilar data. Moreover, it is not known which animal model predicts the protective potency of vaccines for humans. Accordingly, animal models should be developed on the basis of an understanding of the key events in the pathogenesis of tuberculosis in humans. Vaccines such as bacille Calmette-Guérin (BCG) appear to protect against tuberculosis by inhibiting the bacillemic phase of primary infection with virulent tubercle bacilli; therefore, such vaccines can be expected to provide protection against tuberculosis developing via the endogenous reactivation pathway but not against disease developing via the exogenous reinfection pathway. To protect against disease developing by the latter pathway, it would appear that new vaccines produced by recombinant DNA technology or through use of monoclonal antibody would have to inhibit the implantation of bacilli at the portal of entry in the lungs. Experience with BCG vaccine indicates that factors other than the inherent potency of the vaccine play a decisive role in the outcome of vaccine trials in humans. These same factors will probably influence field trials of any new generation of tuberculosis vaccines.
A Polymer Scaffold for Protein Oligomerization (2004)

We report the design and synthesis of well-defined polymers for the noncovalent oligomerization of proteins. The reported scaffolds, which were generated by atom-transfer radical polymerization (ATRP), take advantage of the well-characterized interaction between a Ni2+ complex and an oligohistidine sequence (His tag). Thus, our polymers are designed to facilitate the oligomerization of any protein possessing a His tag. We demonstrate that they can oligomerize fibroblast growth factor-8b (FGF-8b) and promote FGF-8b-mediated cell proliferation in the absence of heparin.
A Theoretical Model of Type I Collagen Proteolysis by Matrix Metalloproteinase (MMP) 2 and Membrane Type 1 MMP in the Presence of Tissue Inhibitor of Metalloproteinase 2 (2004)

One well documented family of enzymes responsible for the proteolytic processes that occur in the extracellular matrix is the soluble and membrane-associated matrix metalloproteinases. Here we present the first theoretical model of the biochemical network describing the proteolysis of collagen I by matrix metalloproteinases 2 (MMP2) and membrane type 1 matrix metalloproteinases (MT1-MMP) in the presence of the tissue inhibitor of metalloproteinases 2 (TIMP2) in a bulk, cell-free, well stirred environment. The model can serve as a tool for describing quantitatively the activation of the MMP2 proenzyme (pro-MMP2), the ectodomain shedding of MT1-MMP, and the collagenolysis arising from both of the enzymes. We show that pro-MMP2 activation, a process that involves a trimer formation of the proenzyme with TIMP2 and MT1-MMP, is suppressed at high inhibitor levels and paradoxically attains maximum only at intermediate TIMP2 concentrations. We also calculate the conditions for which pro-MMP2 activation is maximal. Furthermore we demonstrate that the ectodomain shedding of MT1-MMP can serve as a mechanism controlling the MT1-MMP availability and therefore the pro-MMP2 activation. Finally the proteolytic synergism of MMP2 and MT1-MMP is introduced and described quantitatively. The model provides us a tool to determine the conditions under which the synergism is optimized. Our approach is the first step toward a more complete description of the proteolytic processes that occur in the extracellular matrix and include a wider spectrum of enzymes and substrates as well as naturally occurring or artificial inhibitors.
Differentiation of neurons from neural precursors generated in floating spheres from embryonic stem cells (2009)

Neural progenitors were produced from murine ES cells by a novel method that induced neuroectoderm cells by a combination of nonadherent conditions and serum starvation, in contrast to the embryoid body method in which neuroectoderm cells must be selected after formation of all three germ layers.
Immunomodulatory effect of a plasmid expressing CD40 ligand on DNA vaccination against human immunodeficiency virus type‐1 (1999)

CD40 ligand is a costimulatory molecule which acts a potent immunomodulator. We found the mice inoculated with human CD40 ligand expression plasmid (pMEhCD40L) combined with human immunodeficiency virus type-1 (HIV-1) DNA vaccine exhibited both humoral and cellular antigen-specific immunological enhancement. The expression of hCD40L induced predominantly antigen-specific immunoglobulin G (IgG) antibody response while it failed to induce mucosal IgA response. Delayed-type hypersensitivity (DTH) and cytotoxic T lymphocyte (CTL) activity were induced in a dose-dependent manner. Examination of the relative levels of the two IgG subclasses showed that co-injection of pMEhCD40L enhanced IgG2a response without suppressing IgG1 response. Similarly, the expression of pMEhCD40L enhanced not only T helper 1 (Th1)- but also Th2-type cytokine production. In conclusion, co-inoculation of pMEhCD40L with DNA vaccine was shown to be a useful way to enhance CTL responses without suppressing the humoral immune response in acquired immune deficiency syndrome (AIDS) patients.
Sulfamide Synthesis via Pd-Catalysed Cross-Coupling (2004)

A novel efficient procedure for the improved synthesis of aryl-substituted sulfamides via a Pd-catalysed arylation of sulf­amide is reported.
A distributed scientific data archive using the Web, XML and SQL/MED (1999)

We have developed a web-based architecture and user interface for fast storage, searching and retrieval of large, distributed, files resulting from scientific simulations. We demonstrate that the new DATALINK type defined in the draft SQL Management of External Data Standard can help to overcome problems associated with limited bandwidth when trying to archive large files using the web. We also show that separating the user interface specification from the user interface processing can provide a number of advantages. We provide a tool to generate automatically a default user interface specification, in the form of an XML document, for a given database. This facilitates deployment of our system by users with little web or database development experience. The XML document can be customised to change the appearance of the interface.
Monte Carlo Sampling for Regret Minimization in Extensive Games (2009)

Sequential decision-making with multiple agents and imperfect information is commonly modeled as an extensive game. One efficient method for computing Nash equilibria in large, zero-sum, imperfect information games is counterfactual regret minimization (CFR). In the domain of poker, CFR has proven effective, particularly when using a domain-specific augmentation involving chance outcome sampling. In this paper, we describe a general family of domain-independent CFR sample-based algorithms called Monte Carlo counterfactual regret minimization (MCCFR) of which the original and poker-specific versions are special cases. We start by showing that MCCFR performs the same regret updates as CFR on expectation. Then, we introduce two sampling schemes: outcome sampling and external sampling, showing that both have bounded overall regret with high probability. Thus, they can compute an approximate equilibrium using self-play. Finally, we prove a new tighter bound on the regret for the original CFR algorithm and relate this new bound to MCCFR's bounds. We show empirically that, although the sample-based algorithms require more iterations, their lower cost per iteration can lead to dramatically faster convergence in various games.
An Overview of Reeled-Tubing-Conveyed society Production Logging Capabilities in California (1990)

ABSTRACT Horizontal well completions have increased production efficiencies for operators and are challenging service companies to develop new wellbore analysis and maintenance techniques. Reeled tubing has played a key role in the development of horizontal well servicing techniques. Especially important is the ability to run a continuous tubing string through horizontal intervals while the string is under compression. By installing an electric wireline inside reeled tubing and using the reeled tubing as a transport and placement mechanism, many standard wireline services can be performed in highly deviated and horizontal wellbores, even in live well conditions. Reeled-tubing-conveyed wireline services performed to date in California include production logs, cement bond logs, casing inspection logs, and perforating. Reeled-tubing-conveyed wireline systems have been successfully deployed in California wells with deviations of 90°, over horizontal ramps of 7,200 ft (2,195 m) in length, at depths of 13,900 ft (4,237 m), and with wellhead pressures of 2,000 psi (13,790 kPa). A reeled-tubing-conveyed wireline system is described in detail and two field examples are discussed.
Metabolism of dihalomethanes to carbon monoxide. I. In vivo studies. (1974)

Dibromomethane was metabolized to carbon monoxide and inorganic bromide by a rat liver microsomal fraction requiring both NADPH and molecular oxygen. This biotransformation was characterized with respect to time course, microsomal protein concentration, pH, and temperature. The metabolism of dihalomethanes to carbon monoxide followed the halide order; thus, diiodomethane yielded the greatest amount of carbon monoxide, whereas dichloromethane yielded the smallest amount. A KM of approximately 16 mM was established for dibromomethane while the Vmax was found to be about 8 nmol of CO per mg of microsomal protein per min. Cytochrome P-450 was found to bind dibromomethane to produce a type I binding spectrum. Pretreatment with phenobarbital increased both microsomal cytochrome P-450 levels and the rate of conversion of dibromomethane to carbon monoxide. Pretreatment with cobaltous chloride or storage of microsomal preparations at 4 degrees C resulted in parallel reductions of both cytochrome P-450 levels and the rate of formation of carbon monoxide from dibromomethane. SKF 525-A, ethylmorphine, and hexobarbital inhibited the conversion of dibromomethane to carbon monoxide. Microsomal preparations from rat lung metabolized this substrate at about 18% of the rate found in liver microsomes. The requirement for both NADPH and molecular oxygen, the inhibitory effects of SKF 525-A, ethylmorphine, and hexobarbital on the production of carbon monoxide from dibromomethane, and the correlation established between microsomal cytochrome P-450 levels and the rate of metabolism of dihalomethanes to carbon monoxide after treatments that alter the cytochrome to P-450 content suggest that these compounds are metabolized to carbon monoxide via a cytochrome P-450-dependent system.