gebhart/patrepeval huggingface dataset:
Body composition and skin temperature variation (1990)

Temperature variations near four common torso skin temperature sites were measured on 17 lightly clad subjects exposed to ambient temperatures of 28, 23, and 18 degrees C. Although variations in skin temperature exceeding 7 degrees C over a distance of 5 cm were observed on individuals, the mean magnitude of these variations was 2-3 degrees C under the coolest condition and less at the warmer temperatures. There was no correlation between the temperature variation and skinfold thickness at a site or with estimations of whole body fat content. These findings imply that errors in mean skin temperature measurement could arise from probe mislocation and/or subcutaneous fat distribution and that the problem becomes more acute with increasing cold stress. However, the magnitudes of these errors cannot be easily predicted from common anthropometric measurements.
Polymorphism and regulation of the spxB (pyruvate oxidase) virulence factor gene by a CBS‐HotDog domain protein (SpxR) in serotype 2 Streptococcus pneumoniae (2007)

spxB-encoded pyruvate oxidase is a major virulence factor of Streptococcus pneumoniae. During aerobic growth, SpxB synthesizes H2O2 and acetyl phosphate, which play roles in metabolism, signalling, and oxidative stress. We report here the first cis- and trans-acting regulatory elements for spxB transcription. These elements were identified in a genetic screen for spontaneous mutations that caused colonies of strain D39 to change from a semitransparent to an opaque appearance. Six of the seven opaque colonies recovered (frequency approximately 3 x 10(-5)) were impaired for SpxB function or expression. Two mutations changed amino acids in SpxB likely required for cofactor or subunit binding. One mutation defined a cis-acting adjacent direct repeat required for optimal spxB transcription. The other three spontaneous mutations created the same frameshift near the start of the trans-acting spxR regulatory gene. The SpxR protein contains helix-turn-helix, CBS and HotDog domains implicated in binding DNA, adenosyl compounds, and CoA-containing compounds respectively, and suggest that SpxR positively regulates spxB transcription in response to energy and metabolic state. Microarray analyses unexpectedly demonstrated that SpxR also positively regulates the strH exoglycosidase gene, which, like spxB, has been implicated in colonization. Finally, SpxR is required for full virulence in a murine model of infection.
Virtual Reality–Augmented Rehabilitation for Patients Following Stroke (2002)

The outcomes suggest that VR may be useful to augment rehabilitation of the upper limb in patients in the chronic phase following stroke.
Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö 6976 (1993)

Indolocarbazoles have been identified as novel inhibitors of protein kinase C (PKC), with Gö 6976 as one of its most potent and selective representatives. Recombinant PKC isozymes alpha, beta 1, delta, epsilon, and zeta were used in in vitro kinase assays to investigate Gö 6976 with respect to isozyme-specific PKC inhibition. Gö 6850, identical with GF 109203X, another PKC-specific kinase inhibitor, was included in this study as a reference compound. Nanomolar concentrations of the indolocarbazole Gö 6976 inhibited the Ca(2+)-dependent isozymes alpha and beta 1, whereas even micromolar concentration of Gö 6976 had no effect on the kinase activity of the Ca(2+)-independent PKC subtypes delta, epsilon, and zeta. In contrast, the bisindolymaleimide Gö 6850 inhibited all PKC isozymes, however, with a ranked order of potency (alpha > beta 1 > epsilon > delta > zeta). Kinetic analysis revealed that PKC inhibition by Gö 6976 was competitive with respect to ATP, non-competitive with respect to the protein substrate, and mixed type with respect to phosphatidylserine. Further experiments in the presence of different amounts of free Ca2+ indicated that interference with Ca2+ or its binding site is not responsible for the differential inhibition of PKC isozymes by Gö 6976.
Elaboration of a 3.6-kilodalton lipooligosaccharide, antibody against which is absent from human sera, is associated with serum resistance of Neisseria gonorrhoeae (1985)

Neisseria gonorrhoeae strains that resist lysis by normal human sera (NHS) do so, in part, because NHS contain immunoglobulin M (IgM) specific for lipooligosaccharide (LOS) antigens of serum-sensitive strains, but lack antibodies for LOS antigens that can serve as loci for immune lysis of serum-resistant (serr) strains. We used a monoclonal antibody (McAb), specific for an epitope within a 3.6-kilodalton (kDa) component of Neisseria meningitidis L8 LOS, that binds a 3.6-kDa gonococcal LOS component so that we could explore further serr gonococcal strains. The McAb bound to the LOS of 6 of 7 serr of strains but not to the LOS of 0 of 14 serum-sensitive and serum-intermediate gonococcal strains of diverse origin. We studied three serr strains further. Strain 7134 does not elaborate the 3.6-kDa LOS component and does not bind the McAb; strains WR220 and WR302 do elaborate the 3.6-kDa LOS component. The titer (log2) at which the McAb, diluted in NHS, lysed strain WR220 was 7.7; for WR302 it was 3.7, and for 7134 it was 0. Addition of McAb to NHS caused increased classical and alternative-pathway C3 deposition onto strain WR220, but only classical-pathway-activated C3 deposition onto strain WR302. The difference in lytic effectiveness of the McAb for the two strains, therefore, may result from differences in alternative-pathway augmentation of McAb-dependent classical-pathway activation on their surfaces. None of 40 randomly selected normal young adults had serum antibody that could compete with the McAb for binding to WR220 LOS in a solid-phase RIA. We conclude that the 3.6-kDa LOS component is commonly expressed by serr strains of N. gonorrhoeae and that antibody to it would be lytic if present in human serum, but that it is infrequently, if ever, present. As a result, strains elaborating this LOS are resistant to lysis by NHS.
Person-to-Person Household and Nosocomial Transmission of Andes Hantavirus, Southern Chile, 2011 (2014)

Andes hantavirus (ANDV) causes hantavirus cardiopulmonary syndrome in Chile and is the only hantavirus for which person-to-person transmission has been proven. We describe an outbreak of 5 human cases of ANDV infection in which symptoms developed in 2 household contacts and 2 health care workers after exposure to the index case-patient. Results of an epidemiologic investigation and sequence analysis of the virus isolates support person-to-person transmission of ANDV for the 4 secondary case-patients, including nosocomial transmission for the 2 health care workers. Health care personnel who have direct contact with ANDV case-patients or their body fluids should take precautions to prevent transmission of the virus. In addition, because the incubation period of ANDV after environmental exposure is longer than that for person-to-person exposure, all persons exposed to a confirmed ANDV case-patient or with possible environmental exposure to the virus should be monitored for 42 days for clinical symptoms.
Computer-generated holographic component with optimum light efficiency (1984)

A filter combination consisting of a lens and two pure phase filters, situated in the two focal planes, is discussed. This element, which we call a tandem component, apparently does not absorb any light; in other words, the tandem component is capable of shaping wave fronts with 100% efficiency independent of the object function. We describe a basic configuration and outline its space-variant system properties. The tandem component can be used for many of the standard applications of computer-generated holograms and possibly for some new types as well in view of its space variance.
Use of Cultured Embryonal Chick Epiphyseal Chondrocytes as Grafts for Defects in Chick Articular Cartilage (1987)

Full-thickness defects in articular cartilage were repaired with cultured homologous embryonic chick epiphyseal chondrocytes embedded in a biological resorbable immobilization vehicle (BRIV). This graft was successfully transplanted in mechanically induced defects in the surface of condylar articular cartilage of the tibiotarsal joint of four-month-old roosters. Healing of the defects was observed macroscopically, histologically, and histochemically and with the use of biochemical analyses for six months. Chondrocyte proliferation was seen 48 hours after implantation, and a hyaline cartilage matrix surrounding the cells was present two weeks later. Within eight weeks, the defects were completely filled with hyaline cartilage, which integrated smoothly with the neighboring cartilage without the formation of fibrous tissue at the interface. The cell content and rate of proteoglycan synthesis remained high for four months, then declined slowly to the level of the surrounding cartilage. Six months after transplantation, the cartilaginous tissue in the wound at levels below the ossification front showed penetration by vascular elements and young bone trabeculae at the margins of the reparative tissue. No signs of immunogenic rejection of the implants were observed. These results may be related to the employment of a capable source of cells, i.e., cultured chondrocytes characterized by a high mitotic rate and an early stage of development. The transplanted cells grew well and maintained their initial rate of proliferation, with definite maturation and transformation. The resulting cartilage was structurally reorganized according to the host pattern and under the influence of multitudinous environmental conditions. The articular zone preserved its cartilaginous phenotype, whereas the subchondral regions were transformed into bone.
Antifungal drug susceptibilities of oral Candida dubliniensis isolates from human immunodeficiency virus (HIV)-infected and non-HIV-infected subjects and generation of stable fluconazole-resistant derivatives in vitro (1997)

Candida dubliniensis is a recently described species of Candida associated with oral candidiasis in human immunodeficiency virus (HIV)-infected individuals. Nineteen oral isolates of C. dubliniensis recovered from 10 HIV-positive and 4 HIV-negative individuals and one vaginal isolate from an additional HIV-negative subject were assessed for fluconazole susceptibility by broth microdilution (BMD), hyphal elongation assessment, and Etest. The susceptibilities of these 20 isolates to itraconazole and amphotericin B and of 10 isolates to ketoconazole were also determined by BMD only. Sixteen of the C. dubliniensis isolates were susceptible to fluconazole (MIC range, 0.125 to 1.0 microgram ml-1), and four (recovered from two AIDS patients) were fluconazole resistant (MIC range, 8 to 32 micrograms ml-1). Fluconazole susceptibility data obtained by hyphal elongation assessment correlated well with results obtained by BMD, but the corresponding Etest MIC results were one to four times higher. All of the isolates tested were found to be sensitive to itraconazole, ketoconazole, and amphotericin B. Sequential exposure of two fluconazole-sensitive (MIC, 0.5 microgram ml-1) C. dubliniensis isolates to increasing concentrations of fluconazole in agar medium resulted in the recovery of derivatives which expressed a stable fluconazole-resistant phenotype (BMD-determined MIC range, 16 to 64 micrograms ml-1), even after a minimum of 10 consecutive subcultures on drug-free medium and following prolonged storage at -70 degrees C. The clonal relationship between the parental isolates and their respective fluconazole-resistant derivatives was confirmed by genomic DNA fingerprinting and karyotype analysis. The results of this study demonstrate that C. dubliniensis is inherently susceptible to commonly used antifungal drugs, that fluconazole resistance does occur in clinical isolates, and that stable fluconazole resistance can be readily induced in vitro following exposure to the drug.
Synthesis of 2,5-Diformylfuran and Furan-2,5-Dicarboxylic Acid by Catalytic Air-Oxidation of 5-Hydroxymethylfurfural. Unexpectedly Selective Aerobic Oxidation of Benzyl Alcohol to Benzaldehyde with Metal/Bromide Catalysts (2001)

The alcohol group of hydroxymethylfurfural (compound 1, HMF) is preferentially oxidized by dioxygen and metal/bromide catalysts [Co/Mn/Br, Co/Mn/Zr/Br; Co/Mn=Br/(Co+Mn) = 1.0 mol/mol] to form the dialdehyde, 2,5-diformylfuran (compound 2, DFF) in 57% isolated yield. HMF can be also oxidized, via a network of identified intermediates, to the highly insoluble 2,5-furandicarboxylic acid (compound 5, FDA) in 60% yield. For comparison, benzyl alcohol gives benzaldehyde in 80% using the same catalyst system. Over-oxidation (to CO2) of HMF is much higher than that of the benzyl alcohol but can be greatly reduced by increasing catalyst concentration.
A Chemical Study of Burley Tobacco Flavour (Nicotiana tabacum L.). I. Volatile to medium‐volatile constituents (b.p. ≦ 84°/0.001 Torr (1972)

Abstract The fraction b.p. ≦ 84° (0.001 Torr) from Burley tobacco condensate was carefully investigated using fractional distillation and preparative column and gas liquid chromatography aided by GLC/MS coupling. Among the 193 compounds thus separated and characterized by their spectral data, 81 were newly identified tobacco constituents. Most of the compounds isolated in the course of this work display flavouring properties which make them highly suitable for improving the flavour and aroma of tobacco and tobacco smoke.
Outcomes Following Endovascular vs Open Repair of Abdominal Aortic Aneurysm<subtitle>A Randomized Trial</subtitle> (2009)

clinicaltrials.gov Identifier: NCT00094575.
Synthesis, Anti-Human Immunodeficiency Virus and Anti-Hepatitis B Virus Activities of Novel Oxaselenolane Nucleosides (1997)

ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTSynthesis, Anti-Human Immunodeficiency Virus and Anti-Hepatitis B Virus Activities of Novel Oxaselenolane NucleosidesJinfa Du, Sergey Surzhykov, Ju Sheng Lin, M. Gary Newton, Yung-Chi Cheng, Raymond F. Schinazi, and Chung K. ChuView Author Information Department of Medicinal Chemistry, College of Pharmacy, and Department of Chemistry, The University of Georgia, Athens, Georgia 30602, Department of Pharmacology, School of Medicine, Yale University, New Haven, Connecticut 06510, and Department of Pediatrics, Emory University School of Medicine/VA Medical Center, Decatur, Georgia 30033 Cite this: J. Med. Chem. 1997, 40, 19, 2991–2993Publication Date (Web):September 12, 1997Publication History Received5 June 1997Published online12 September 1997Published inissue 1 September 1997https://pubs.acs.org/doi/10.1021/jm9703698https://doi.org/10.1021/jm9703698rapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views417Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Lactones,Mixtures,Nucleic acids,Organic acids,Redox reactions Get e-Alerts
Seeding induced by α‐synuclein oligomers provides evidence for spreading of α‐synuclein pathology (2009)

Lewy bodies, alpha-synuclein (alpha-syn) immunopositive intracellular deposits, are the pathological hallmark of Parkinson's disease (PD). Interestingly, Lewybody-like structures have been identified in fetal tissue grafts about one decade after transplantation into the striatum of PD patients. One possible explanation for the accelerated deposition of alpha-syn in the graft is that the aggregation of alpha-syn from the host tissue to the graft is spread by a prion disease-like mechanism. We discuss here an in vitro model which might recapitulate some aspects of disease propagation in PD. We found here that in vitro-generated alpha-syn oligomers induce transmembrane seeding of alpha-syn aggregation in a dose- and time-dependent manner. This effect was observed in primary neuronal cultures as well as in neuronal cell lines. The seeding oligomers were characterized by a distinctive lithium dodecyl sulfate-stable oligomer pattern and could be generated in a dynamic process out of pore-forming oligomers. We propose that alpha-syn oligomers form as a dynamic mixture of oligomer types with different properties and that alpha-syn oligomers can be converted into different types depending on the brain milieu conditions. Our data indicate that extracellular alpha-syn oligomers can induce intracellular alpha-syn aggregation, therefore we hypothesize that a similar mechanism might lead to alpha-syn pathology propagation.
In vitro and in vivo stability and anti-tumour efficacy of an anti-EGFR/anti-CD3 F(ab')2 bispecific monoclonal antibody (1995)

The in vitro and in vivo stability and anti-tumour efficacy of the anti-EGFR/anti-CD3 bispecific monoclonal antibody (biMAb), M26.1, were analysed. The interaction of the intact biMAb with Fc receptor I (Fc gamma RI) present on human leucocytes was not observed when the antibody was used as an F(ab')2 fragment. A CD8+ T-cell clone coated with M26.1 F(ab')2 was as effective as the intact biMAb in inducing IGROV1 target cell lysis when tested in a 51Cr-release assay. Variable levels of reduction of F(ab')2 to monovalent F(ab') were observed upon incubation with human ovarian cancer ascitic fluid (OCAF) or with human glioblastoma cavity fluid (GCF), but not with mouse or human sera. Activated lymphocytes coated with F(ab')2 and incubated in vitro with GCF or OCAF for 24 and 48 h respectively maintained their targeting. Thus, the F(ab')2, when present as a soluble molecule, but not when bound to T cells, might lose some functional activity as a consequence of partial reduction to F(ab'). In normal mice, M26.1 F(ab')2 retained full cytotoxic activity in the circulation, and clearance values were similar to those obtained with parental and other MAb F(ab')2. Treatment of IGROV1 tumour-bearing mice with activated human lymphocytes coated with the M26.1 F(ab')2 significantly prolonged survival of the animals compared with tumour-bearing untreated and control mice treated with lymphocytes or F(ab')2 alone. Together, these results suggest the clinical usefulness of bispecific M26.1 F(ab')2 as a targeting agent for local treatment of tumours such as glioma and ovarian cancers that express variable levels of epidermal growth factor receptor (EGFR).
Neurosteroid Analogs. 3. The Synthesis and Electrophysiological Evaluation of Benz[e]indene Congeners of Neuroactive Steroids Having the 5.beta.-Configuration (1995)

A series of 7-(2-hydroxyethyl)benz[e]indene analogues of 3 alpha-hydroxy-5 beta-pregnan-20-one (7), a neuroactive steroid known to be a positive allosteric modulator of GABAA receptor function, was prepared. Electrophysiological measurements carried out on cultured rat hippocampal neurons were used to evaluate the modulatory effects of the analogues on GABAA receptor function. Analogues were tested for their ability to potentiate 1 microM GABA-mediated chloride currents and for their ability to directly gate chloride currents at this ligand-gated ion channel. Active analogues typically enhanced GABA-mediated currents at concentrations below those required to directly gate chloride currents. The dose-response relationships for potentiation of 1 microM GABA-mediated chloride currents were studied for [3S-(3 alpha, 3a alpha, 5a beta, 7 beta, 9a alpha, 9b beta)]-1- [dodecahydro-7-(2-hydroxyethyl)-3a-methyl-1H-benz[e]inden-3- yl]ethanone (3), steroid 7, 3 alpha-hydroxy-5 alpha-pregnan-20-one (5), and the analogous 7 alpha-(2-hydroxyethyl)benz[e]indene analogue of steroid 5 (compound 1). Compound 3 was the most active potentiator (EC50 = 0.017 microM) of GABA-mediated current. The direct gating actions of compound 3 were not observed at a concentration of 1 microM, but were observed at a concentration of 10 microM.
Cooperative Spectrum Sensing for Cognitive Radios Using Kriged Kalman Filtering (2010)

A cooperative cognitive radio (CR) sensing problem is considered, where a number of CRs collaboratively detect the presence of primary users (PUs) by exploiting the novel notion of channel gain (CG) maps. The CG maps capture the propagation medium per frequency from any point in space and time to each CR user. They are updated in real-time using Kriged Kalman filtering (KKF), a tool with well-appreciated merits in geostatistics. In addition, the CG maps enable tracking the transmit-power and location of an unknown number of PUs, via a sparse regression technique. The latter exploits the sparsity inherent to the PU activities in a geographical area, using an <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">ℓ</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> -norm regularized, sparsity-promoting weighted least-squares formulation. The resulting sparsity-cognizant tracker is developed in both centralized and distributed formats, to reduce computational complexity and memory requirements of a batch alternative. Numerical tests demonstrate considerable performance gains achieved by the proposed algorithms .
Transcriptome Analysis of a cDNA Library from Adult Human Epididymis (2008)

Mammalian Gene Collection (MGC) verified over 9,000 human full-ORF genes and FLJ Program reported 21,243 cDNAs of which 14,409 were unique ones and 5,416 seemed to be protein-coding. The pity is that epididymis cDNA library was missing in their sequencing target list. Epididymis is a very important male accessory sex organ for sperm maturation and storage. Fully differentiated spermatozoa left from testis acquire their motility and capacity for fertilization via interactions with the epididymal epithelium duct lumen during passage through this convoluted duct. Here, we report that 20,000 clones from a healthy male epididymis cDNA library have been sequenced. The sequencing data provided 8,234 known sequences and 650 unknown cDNA fragments. Hundred and six of 650 unknown cDNA clone inserts were randomly selected for fully sequencing. There were 25 unknown unique sequences and 19 released but unreported sequences came out. By northern blot analysis, four sequences randomly selected from the 19 released sequences with no known function showed positive mRNA signals in epididymis and testis. The signals for three of six from those unknown group showed as epididymis abundant in a region-specific manner but not in the testis and other tissues tested. All the sequencing data will be available on the website www.sdscli.com.