gebhart/patrepeval huggingface dataset:
THE ATOMIC ARRANGEMENT IN GLASS (1932)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE ATOMIC ARRANGEMENT IN GLASSW. H. ZachariasenCite this: J. Am. Chem. Soc. 1932, 54, 10, 3841–3851Publication Date (Print):October 1, 1932Publication History Published online1 May 2002Published inissue 1 October 1932https://pubs.acs.org/doi/10.1021/ja01349a006https://doi.org/10.1021/ja01349a006research-articleACS PublicationsRequest reuse permissionsArticle Views8685Altmetric-Citations2280LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Current Tools for Assisting Intelligent Agents in Real-time Decision Making (1999)

Decision making is something with which we are all familiar. Each day we find ourselves in situations where we have more than one alternative to choose from. Most of our everyday decisions require little or no reflection. Yet, other decisions are more complex and may require thorough analysis. The field of decision analysis studies the application of decision theory to actual decision problems. Decision theory is the mathematical foundation for decision making, which combines probability theory and utility theory in order to describe what constitutes a rational decision. Today there are numerous tools for decision analysis that run on ordinary PCs. These make it easier for human decision makers to structure a decision problem, and help visualize the effects of possible outcomes. However, not only humans are decision makers. Intelligent agents are also faced with decision problems. This thesis investigates if any of the computerized tools for decision analysis can be used by intelligent agents. Special attention is given to real-time domains, where data that decisions depend on vary rapidly. These domains require quick responses from the tools. Otherwise the result of a decision analysis will be based on data that is no longer accurate. The objective of the thesis is to provide results indicating if any of the available tools for decision analysis are fast enough for use in real-time domains.
Living carbocationic polymerization. 56. Polyisobutylene-containing block polymers by sequential monomer addition. 8. Synthesis, characterization, and physical properties of poly(indene-b-isobutylene-b-indene) thermoplastic elastomers (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLiving carbocationic polymerization. 56. Polyisobutylene-containing block polymers by sequential monomer addition. 8. Synthesis, characterization, and physical properties of poly(indene-b-isobutylene-b-indene) thermoplastic elastomersJoseph P. Kennedy, Sanjeev Midha, and Yasuo TsunogaeCite this: Macromolecules 1993, 26, 3, 429–435Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://pubs.acs.org/doi/10.1021/ma00055a004https://doi.org/10.1021/ma00055a004research-articleACS PublicationsRequest reuse permissionsArticle Views219Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
4-Hydroxylation of estradiol by human uterine myometrium and myoma microsomes: implications for the mechanism of uterine tumorigenesis. (1995)

Estradiol is converted to catechol estrogens via 2- and 4-hydroxylation by cytochrome P450 enzymes. 4-Hydroxyestradiol elicits biological activities distinct from estradiol, most notably an oxidant stress response induced by free radicals generated by metabolic redox cycling reactions. In this study, we have examined 2- and 4-hydroxylation of estradiol by microsomes of human uterine myometrium and of associated myomata. In all eight cases studied, estradiol 4-hydroxylation by myoma has been substantially elevated relative to surrounding myometrial tissue (minimum, 2-fold; mean, 5-fold). Estradiol 2-hydroxylation in myomata occurs at much lower rates than 4-hydroxylation (ratio of 4-hydroxyestradiol/2-hydroxyestradiol, 7.9 +/- 1.4) and does not significantly differ from rates in surrounding myometrial tissue. Rates of myometrial 2-hydroxylation of estradiol were also not significantly different from values in patients without myomata. We have used various inhibitors to establish that 4-hydroxylation is catalyzed by a completely different cytochrome P450 than 2-hydroxylation. In myoma, alpha-naphthoflavone and a set of ethynyl polycyclic hydrocarbon inhibitors (5 microM) each inhibited 4-hydroxylation more efficiently (up to 90%) than 2-hydroxylation (up to 40%), indicating > 10-fold differences in Ki (<0.5 microM vs. > 5 microM). These activities were clearly distinguished from the selective 2-hydroxylation of estradiol in placenta by aromatase reported previously (low Km, inhibition by Fadrozole hydrochloride or ICI D1033). 4-Hydroxylation was also selectively inhibited relative to 2-hydroxylation by antibodies raised against cytochrome P450 IB1 (rat) (53 vs. 17%). These data indicate that specific 4-hydroxylation of estradiol in human uterine tissues is catalyzed by a form(s) of cytochrome P450 related to P450 IB1, which contribute(s) little to 2-hydroxylation. This enzyme(s) is therefore a marker for uterine myomata and may play a role in the etiology of the tumor.
Fcγ receptor pathways during active and passive immunization (2015)

IgG antibodies are actively produced in response to antigenic challenge or passively administered as an effective form of immunotherapy to confer immunity against foreign antigens. Their protective activity is mediated through their bifunctional nature: a variable Fab domain mediates antigen-binding specificity, whereas the constant Fc domain engages Fcγ receptors (FcγRs) expressed on the surface of leukocytes to mediate effector functions. While traditionally considered the invariant domain of an IgG molecule, the Fc domain displays remarkable structural heterogeneity determined primarily by differences in the amino acid sequence of the various IgG subclasses and by the composition of the complex, Fc-associated biantennary N-linked glycan. These structural determinants regulate the conformational flexibility of the IgG Fc domain and affect its capacity to interact with distinct types of FcγRs (type I or type II FcγRs). FcγR engagement activates diverse downstream immunomodulatory pathways with pleiotropic functional consequences including cytotoxicity and phagocytosis of IgG-coated targets, differentiation and activation of antigen presenting cells, modulation of T-cell activation, plasma cell survival, and regulation of antibody responses. These functions highlight the importance of FcγR-mediated pathways in the modulation of adaptive immune responses and suggest a central role for IgG-FcγR interactions during active and passive immunization.
Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments (1996)

Many methods for detecting model genetically engineered microorganisms (GEMs) in experimental ecosystems rely on cultivation of introduced cells. In this study, survival of Escherichia coli was monitored with the green fluorescent protein (GFP) gene. This approach allowed enumeration of GEMs by both plating and microscopy. Use of the GFP-marked GEMs revealed that E. coli persisted in stream water at higher densities as determined microscopically than as determined by CFU enumeration. The GFP gene did not negatively impact the fitness of the host strain.
Matrix Metalloproteinases Generate Angiostatin: Effects on Neovascularization (1998)

Angiostatin, a cleavage product of plasminogen, has been shown to inhibit endothelial cell proliferation and metastatic tumor cell growth. Recently, the production of angiostatin has been correlated with tumor-associated macrophage production of elastolytic metalloproteinases in a murine model of Lewis lung cell carcinoma. In this report we demonstrate that purified murine and human matrix metalloproteinases generate biologically functional angiostatin from plasminogen. Macrophage elastase (MMP-12 or MME) proved to be the most efficient angiostatin-producing MMP. MME was followed by gelatinases and then the stomelysins in catalytic efficiency; interstitial collagenases had little capacity to generate angiostatin. Both recombinant angiostatin and angiostatin generated from recombinant MME-treated plasminogen inhibited human microvascular endothelial cell proliferation and differentiation in vitro. Finally, employing macrophages isolated from MME-deficient mice and their wild-type littermates, we demonstrate that MME is required for the generation of angiostatin that inhibits the proliferation of human microvascular endothelial cells.
Network of inflammatory cytokines and correlation with disease activity in ulcerative colitis (1998)

UC appears to be a chronic inflammatory disease characterized by high production of all four proinflammatory cytokines (IL1beta, TNFalpha, IL6, and IL8). These results suggest that colonic perfusion may be a suitable method to evaluate the local anticytokine properties of new drugs, in correlation with disease activity and systemic markers of inflammation.
Variance Reduction in Monte Carlo Counterfactual Regret Minimization (VR-MCCFR) for Extensive Form Games Using Baselines (2019)

Learning strategies for imperfect information games from samples of interaction is a challenging problem. A common method for this setting, Monte Carlo Counterfactual Regret Minimization (MCCFR), can have slow long-term convergence rates due to high variance. In this paper, we introduce a variance reduction technique (VR-MCCFR) that applies to any sampling variant of MCCFR. Using this technique, periteration estimated values and updates are reformulated as a function of sampled values and state-action baselines, similar to their use in policy gradient reinforcement learning. The new formulation allows estimates to be bootstrapped from other estimates within the same episode, propagating the benefits of baselines along the sampled trajectory; the estimates remain unbiased even when bootstrapping from other estimates. Finally, we show that given a perfect baseline, the variance of the value estimates can be reduced to zero. Experimental evaluation shows that VR-MCCFR brings an order of magnitude speedup, while the empirical variance decreases by three orders of magnitude. The decreased variance allows for the first time CFR+ to be used with sampling, increasing the speedup to two orders of magnitude.
A TNF Receptor 2 Selective Agonist Rescues Human Neurons from Oxidative Stress-Induced Cell Death (2011)

Tumor necrosis factor (TNF) plays a dual role in neurodegenerative diseases. Whereas TNF receptor (TNFR) 1 is predominantly associated with neurodegeneration, TNFR2 is involved in tissue regeneration and neuroprotection. Accordingly, the availability of TNFR2-selective agonists could allow the development of new therapeutic treatments of neurodegenerative diseases. We constructed a soluble, human TNFR2 agonist (TNC-scTNF(R2)) by genetic fusion of the trimerization domain of tenascin C to a TNFR2-selective single-chain TNF molecule, which is comprised of three TNF domains connected by short peptide linkers. TNC-scTNF(R2) specifically activated TNFR2 and possessed membrane-TNF mimetic activity, resulting in TNFR2 signaling complex formation and activation of downstream signaling pathways. Protection from neurodegeneration was assessed using the human dopaminergic neuronal cell line LUHMES. First we show that TNC-scTNF(R2) interfered with cell death pathways subsequent to H(2)O(2) exposure. Protection from cell death was dependent on TNFR2 activation of the PI3K-PKB/Akt pathway, evident from restoration of H(2)O(2) sensitivity in the presence of PI3K inhibitor LY294002. Second, in an in vitro model of Parkinson disease, TNC-scTNF(R2) rescues neurons after induction of cell death by 6-OHDA. Since TNFR2 is not only promoting anti-apoptotic responses but also plays an important role in tissue regeneration, activation of TNFR2 signaling by TNC-scTNF(R2) appears a promising strategy to ameliorate neurodegenerative processes.
Type I Interferon-mediated Stimulation of T Cells by CpG DNA (1998)

Immunostimulatory DNA and oligodeoxynucleotides containing unmethylated CpG motifs (CpG DNA) are strongly stimulatory for B cells and antigen-presenting cells (APCs). We report here that, as manifested by CD69 and B7-2 upregulation, CpG DNA also induces partial activation of T cells, including naive-phenotype T cells, both in vivo and in vitro. Under in vitro conditions, CpG DNA caused activation of T cells in spleen cell suspensions but failed to stimulate highly purified T cells unless these cells were supplemented with APCs. Three lines of evidence suggested that APC-dependent stimulation of T cells by CpG DNA was mediated by type I interferons (IFN-I). First, T cell activation by CpG DNA was undetectable in IFN-IR-/- mice. Second, in contrast to normal T cells, the failure of purified IFN-IR-/- T cells to respond to CpG DNA could not be overcome by adding normal IFN-IR+ APCs. Third, IFN-I (but not IFN-gamma) caused the same pattern of partial T cell activation as CpG DNA. Significantly, T cell activation by IFN-I was APC independent. Thus, CpG DNA appeared to stimulate T cells by inducing APCs to synthesize IFN-I, which then acted directly on T cells via IFN-IR. Functional studies suggested that activation of T cells by IFN-I was inhibitory. Thus, exposing normal (but not IFN-IR-/-) T cells to CpG DNA in vivo led to reduced T proliferative responses after TCR ligation in vitro.
Delineation ofMGMTHypermethylation as a Biomarker for Veliparib-Mediated Temozolomide-Sensitizing Therapy of Glioblastoma (2015)

Veliparib statistically significantly enhances (P < .001) the efficacy of TMZ in tumors with MGMT promoter hypermethylation. Based on these data, MGMT promoter hypermethylation is being used as an eligibility criterion for A071102 (NCT02152982), the phase II/III clinical trial evaluating TMZ/veliparib combination in patients with GBM.
Nitrogen isotopic analyses by isotope-ratio-monitoring gas chromatography / mass spectrometry (1994)

Amino acids containing natural-abundance levels of (15)N were derivatized and analyzed isotopically using a technique in which individual compounds are separated by gas chromatography, combusted on-line, and the product stream sent directly to an isotope-ratio mass spectrometer. For samples of N2 gas, standard deviations of ratio measurement were better than 0.1‰ (Units for δ are parts per thousand or per million (‰).) for samples larger than 400 pmol and better than 0.5‰ for samples larger than 25 pmol (0.1‰ (15)N is equivalent to 0.00004 atom % (15)N). Results duplicated those of conventional, batchwise analyses to within 0.05‰. For combustion of organic compounds yielding CO2/N2 ratios between 14 and 28, in particular for N-acetyl n-propyl derivatives of amino acids, δ values were within 0.25‰ of results obtained using conventional techniques and standard deviations were better than 0.35‰. Pooled data for measurements of all amino acids produced an accuracy and precision of 0.04 and 0.23‰, respectively, when 2 mnol of each amino acid was injected on column and 20% of the stream of combustion products was delivered to the mass spectrometer.
Essential role of PKCδ in histone deacetylase inhibitor-induced Epstein–Barr virus reactivation in nasopharyngeal carcinoma cells (2008)

Histone deactylase inhibitors (HDACi) are common chemotherapeutic agents that stimulate Epstein-Barr virus (EBV) reactivation; the detailed mechanism remains obscure. In this study, it is demonstrated that PKCdelta is required for induction of the EBV lytic cycle by HDACi. Inhibition of PKCdelta abrogates HDACi-mediated transcriptional activation of the Zta promoter and downstream lytic gene expression. Nuclear translocation of PKCdelta is observed following HDACi stimulation and its overexpression leads to progression of the EBV lytic cycle. Our study suggests that PKCdelta is a crucial mediator of EBV reactivation and provides a novel insight to study the regulation of the EBV lytic cycle.
Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis (2006)

This comprehensive analysis allows us the identification of genus- specific proteins, species-specific proteins and, more importantly, proteins common among these different sandflies. These results give us insights into the repertoire of salivary proteins that are potential candidates for a vector-based vaccine.
Compact Multiscale Finite Volume Method for Heterogeneous Anisotropic Elliptic Equations (2008)

The multiscale finite volume (MSFV) method is introduced for the efficient solution of elliptic problems with rough coefficients in the absence of scale separation. The coarse operator of the MSFV method is presented as a multipoint flux approximation (MPFA) with numerical evaluation of the transmissibilities. The monotonicity region of the original MSFV coarse operator has been determined for the homogeneous anisotropic case. For grid-aligned anisotropy the monotonicity of the coarse operator is very limited. A compact coarse operator for the MSFV method is presented that reduces to a 7-point stencil with optimal monotonicity properties in the homogeneous case. For heterogeneous cases the compact coarse operator improves the monotonicity of the MSFV method, especially for anisotropic problems. The compact operator also leads to a coarse linear system much closer to an M-matrix. Gradients in the direction of strong coupling vanish in highly anisotropic elliptic problems with homogeneous Neumann boundary data, a condition referred to as transverse equilibrium (TVE). To obtain a monotone coarse operator for heterogeneous problems the local elliptic problems used to determine the transmissibilities must be able to reach TVE as well. This can be achieved by solving two linear local problems with homogeneous Neumann boundary conditions and constructing a third bilinear local problem with Dirichlet boundary data taken from the linear local problems. Linear combination of these local problems gives the MSFV basis functions but with hybrid boundary conditions that cannot be enforced directly. The resulting compact multiscale finite volume (CMSFV) method with hybrid local boundary conditions is compared numerically to the original MSFV method. For isotropic problems both methods have comparable accuracy, but the CMSFV method is robust for highly anisotropic problems where the original MSFV method leads to unphysical oscillations in the coarse solution and recirculations in the reconstructed velocity field.
Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency. (1993)

Enzymatically active human 17 alpha-hydroxylase cytochrome P450 (P450c17) has been expressed in and purified from Escherichia coli. The cDNA containing modifications within the amino-terminal eight codons which are favorable for expression in E. coli, as well as codons for 4 histidine residues at the carboxyl terminus, was placed in the pCWori+ expression vector. The modified human P450c17 was detected spectrophotometrically (400 nmol of P450c17/liter culture) and was found to be integrated into E. coli membranes. This previously inaccessible human P450 was purified to electrophoretic homogeneity (10.7 nmol of P450/mg) from solubilized bacterial membranes using two sequential chromatographic steps, nickel nitrilotriacetate followed by hydroxylapatite. The expected enzymatic activities of human P450c17 were reconstituted by addition of purified rat liver NADPH-cytochrome P450 reductase, giving turnover numbers of 8.0 nmol/min/nmol P450 for pregnenolone, 6.5 nmol/min/nmol P450 for progesterone, 0.06 nmol/min/nmol P450 for 17 alpha-hydroxypregnenolone, and no detectable activity for 17 alpha-hydroxyprogesterone. This system was utilized to study the molecular basis of a novel form of combined 17 alpha-hydroxylase, 17,20-lyase deficiency resulting from compound heterozygous mutations, a missense point mutation Tyr64(TAT)--> Ser (TCT), and an Ile112 duplication (ATCATC). Upon expression of these mutant proteins in E. coli, the Tyr64 mutant has 15% of the wild type 17 alpha-hydroxylase activity, whereas the Ile112 duplication shows no activity, results consistent with the observed clinical phenotype.
Secondary metabolites ofFusarium venenatumstrains with deletions in theTri5gene encoding trichodiene synthetase (2000)

We studied the secondary metabolites of a number of strains of F. venenatum, some of which had the gene for trichodiene synthetase deleted. Under optimum fermentation conditions, wild type isolates produced the trichothecenes diacetoxyscirpenol and related metabolites, isotrichodermin and isotricodermol, the modified trichothecenes sambucinol and apotrichothecene, the sesquiterpenes culmorin and culmorone and derivatives and trace amounts of the cyclic peptide enniatin B. These results are compared to metabolite production by other members of the section Discolor. Strains without trichodiene synthetase produced no trichothecenes or modified trichothecenes. In these strains, production of culmorin family compounds was greatly attenuated despite the elimination of an acetate-derived biosynthetic pathway. This was contrary to expectations. No other metabolites were produced by these strains evidenced by chemical or cytotoxicity analyses of fractionated extracts.