gebhart/patrepeval huggingface dataset:
Measuring Video QoE from Encrypted Traffic (2016)

Tracking and maintaining satisfactory QoE for video streaming services is becoming a greater challenge for mobile network operators than ever before. Downloading and watching video content on mobile devices is currently a growing trend among users, that is causing a demand for higher bandwidth and better provisioning throughout the network infrastructure. At the same time, popular demand for privacy has led many online streaming services to adopt end-to-end encryption, leaving providers with only a handful of indicators for identifying QoE issues.
Issues in the pragmatics of qualitative modeling: lessons learned from a xerographics project (1987)

The photocopier is one of the most complex machines because xerography involves many types of physical phenomena. ARIA is a qualitative simulation of xerography that is intended to teach technicians the reasons behind some of the subtle problems that occur in copiers. This effort to model xerography exposed shortcomings in the techniques of qualitative modeling as applied to complex systems and helped to better understand the impact of certain basic modeling decisions.
Pharmacologic and Therapeutic Applications of alpha2-Adrenoceptor Subtypes (1993)

Heme oxygenases (HO) catabolize free heme, that is, iron (Fe) protoporphyrin (IX), into equimolar amounts of Fe2+, carbon monoxide (CO), and biliverdin. The stress-responsive HO-1 isoenzyme affords protection against programmed cell death. The mechanism ...Read More
Early development of multiple sclerosis is associated with progressive grey matter atrophy in patients presenting with clinically isolated syndromes (2004)

While brain atrophy occurs early in the clinical course of multiple sclerosis, exactly how early, which tissues are affected and the rate at which early atrophy occurs are unclear. Regional brain atrophy was investigated in 58 patients recruited within 3 months of onset of a clinically isolated syndrome (CIS) suggestive of multiple sclerosis, who were followed-up for 3 years. At 3 years, 31 subjects had developed multiple sclerosis as defined by the McDonald criteria, while 27 had not (13 had MRI-visible brain lesions and 14 did not). In those who developed multiple sclerosis, the mean decrease in grey matter fractional volume (GMF, as a fraction of total intracranial volume) was -0.017 (-3.3%) and was significantly larger than in the combined lesion-positive and lesion-negative CIS subjects [-0.005 (-1.1%), P = 0.001]. No decrease in white matter fractional volumes (WMF) was seen. Change in GMF correlated only modestly with the change in T2 lesion volume from baseline to year 3 (r = -0.428, P = 0.004). These results suggest that progressive grey matter, but not white matter, atrophy is seen in the earliest clinically observable stages of relapse onset multiple sclerosis, and this is only moderately related to lesion accumulation. Longer-term follow-up is required to determine whether early grey matter atrophy is associated with subsequent disability or cognitive impairment.
IR microscopy as an early electrical yield indicator in bonded wafer pairs used for 3D integration (2010)

Microscopy of 3D interconnect structures is challenged by the opaque nature of silicon. Infrared (IR) microscopy provides a way of "looking" through silicon where microscopes based on visible wavelengths fail. Perhaps the most prevalent application of IR microscopes in 3D manufacturing is imaging sub-surface features at the interface of a bonded wafer pair. The ability to see through silicon using IR microscopes enables a variety of metrology techniques, including the overlay of circuit layers (e.g., metal 2 to via). IR microscopy is a non-destructive technique and, as such, it is an ideal candidate for in-line metrology for the bonded wafer pairs required for 3D interconnects. This paper reviews overlay metrology capability for an IR microscope. The ability to measure the overlay of bonded wafer pairs according to the 2009 International Technology Roadmap for Semiconductors (ITRS) is demonstrated. Overlay tolerances for a variety of copper interconnect test structures is predicted based on electrical designs, and overlay results are compared to electrical test results. The use of IR microscopy as an early indicator of electrical yield is clearly demonstrated.
Skinning mesh animations (2005)

We extend approaches for skinning characters to the general setting of skinning deformable mesh animations. We provide an automatic algorithm for generating progressive skinning approximations, that is particularly efficient for pseudo-articulated motions. Our contributions include the use of nonparametric mean shift clustering of high-dimensional mesh rotation sequences to automatically identify statistically relevant bones, and robust least squares methods to determine bone transformations, bone-vertex influence sets, and vertex weight values. We use a low-rank data reduction model defined in the undeformed mesh configuration to provide progressive convergence with a fixed number of bones. We show that the resulting skinned animations enable efficient hardware rendering, rest pose editing, and deformable collision detection. Finally, we present numerous examples where skins were automatically generated using a single set of parameter values.
6D hands (2011)

Computer Aided Design (CAD) typically involves tasks such as adjusting the camera perspective and assembling pieces in free space that require specifying 6 degrees of freedom (DOF). The standard approach is to factor these DOFs into 2D subspaces that are mapped to the x and y axes of a mouse. This metaphor is inherently modal because one needs to switch between subspaces, and disconnects the input space from the modeling space. In this paper, we propose a bimanual hand tracking system that provides physically-motivated 6-DOF control for 3D assembly. First, we discuss a set of principles that guide the design of our precise, easy-to-use, and comfortable-to-use system. Based on these guidelines, we describe a 3D input metaphor that supports constraint specification classically used in CAD software, is based on only a few simple gestures, lets users rest their elbows on their desk, and works alongside the keyboard and mouse. Our approach uses two consumer-grade webcams to observe the user's hands. We solve the pose estimation problem with efficient queries of a precomputed database that relates hand silhouettes to their 3D configuration. We demonstrate efficient 3D mechanical assembly of several CAD models using our hand-tracking system.
Tomography via Correlation of Noisy Measurement Records (2013)

We present methods and results of shot-by-shot correlation of noisy measurements to extract entangled state and process tomography in a superconducting qubit architecture. We show that averaging continuous values, rather than counting discrete thresholded values, is a valid tomographic strategy and is in fact the better choice in the low signal-to-noise regime. We show that the effort to measure $N$-body correlations from individual measurements scales exponentially with $N$, but with sufficient signal-to-noise the approach remains viable for few-body correlations. We provide a new protocol to optimally account for the transient behavior of pulsed measurements. Despite single-shot measurement fidelity that is less than perfect, we demonstrate appropriate processing to extract and verify entangled states and processes.
Structural analysis of wild‐type and mutant yeast calmodulins by limited proteolysis and electrospray ionization mass spectrometry (1992)

Calmodulin from Saccharomyces cerevisiae was expressed in Escherichia coli and purified. The purified protein was structurally characterized using limited proteolysis followed by ESI mass spectrometry to identify the fragments. In the presence of Ca2+, yeast calmodulin is sequentially cleaved at arginine 126, then lysine 115, and finally at lysine 77. The rapid cleavage at Arg-126 suggests that the fourth Ca(2+)-binding loop does not bind Ca2+. In the presence of EGTA, yeast calmodulin is more susceptible to proteolysis and is preferentially cleaved at Lys-106. In addition, mutant proteins carrying I100N, E104V or both mutations, which together confer temperature sensitivity to yeast, were characterized. The mutant proteins are more susceptible than wild-type calmodulin to proteolysis, suggesting that each mutation disrupts the structure of calmodulin. Furthermore, whereas wild-type calmodulin is cut at Lys-106 only in the presence of EGTA, this cleavage site is accessible in the mutants in the presence of Ca2+ as well. In these ways, the structural consequence of each mutation mimics the loss of a calcium ion in the third loop. In addition, although wild-type calmodulin binds to four proteins in a yeast crude extract in the presence of Ca2+, the mutants bind only to a subset of these. Thus, the inability to adopt the stable Ca(2+)-bound conformation in the third Ca(2+)-binding loop alters the ability of calmodulin to interact with yeast proteins in a Ca(2+)-dependent manner.
The plasticity of immunoglobulin gene systems in evolution (2006)

The mechanism of recombination-activating gene (RAG)-mediated rearrangement exists in all jawed vertebrates, but the organization and structure of immunoglobulin (Ig) genes, as they differ in fish and among fish species, reveal their capability for rapid evolution. In systems where there can exist 100 Ig loci, exon restructuring and sequence changes of the constant regions led to divergence of effector functions. Recombination among these loci created hybrid genes, the strangest of which encode variable (V) regions that function as part of secreted molecules and, as the result of an ancient translocation, are also grafted onto the T-cell receptor. Genomic changes in V-gene structure, created by RAG recombinase acting on germline recombination signal sequences, led variously to the generation of fixed receptor specificities, pseudogene templates for gene conversion, and ultimately to Ig sequences that evolved away from Ig function. The presence of so many Ig loci in fishes raises interesting questions not only as to how their regulation is achieved but also how successive whole-locus duplications are accommodated by a system whose function in other vertebrates is based on clonal antigen receptor expression.
An FPGA-Based Tightly Coupled Accelerator for Data-Intensive Applications (2014)

Computation beside a data source plays an important role in achieving a high performance with low energy consumption in Big Data processing. In contrast to that of a conventional workload, the processing of Big Data frequently requires that a massive amount of data in distributed storage be scanned. A key technique for reducing energy-consuming processor loads is to install a reconfigurable accelerator that is tightly coupled to a computational resource with interfaces. The accelerator is capable of configuring application-specific hardware modules to allow some logical and arithmetic operations for data stream transmission between interfaces, as well as the offloading of control protocols for communication with other computing nodes or storage. In this paper, an FPGA-based accelerator, which is directly attached to DRAM, the network, and storage, is proposed in order to realize an energy efficient computing system. A simple application that counts the words appearing in the data is implemented to evaluate a prototype system. As the accelerator outperforms by 80.66 to 429 times similar applications executed on an SSD-based Hadoop framework, we confirm that the accelerator's utilization for Big Data processing is beneficial.
Myostatin short interfering hairpin RNA gene transfer increases skeletal muscle mass (2006)

This is the first demonstration that myostatin shRNA gene transfer is a potential strategy to increase muscle mass.
Envelope Glycoprotein Trimers as HIV-1 Vaccine Immunogens (2013)

The HIV-1 envelope glycoprotein spike is the target of neutralizing antibody attack, and hence represents the only relevant viral antigen for antibody-based vaccine design. Various approaches have been attempted to recapitulate Env in membrane-anchored and soluble forms, and these will be discussed here in the context of recent successes and challenges still to be overcome.
Enhancing transdermal delivery of drug-infused particles using electrostatic pulse (2013)

Thesis (Ph.D.)--Boston University&#13;\n&#13;\nPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
Cloning and Functional Characterization of Novel Large Conductance Calcium-activated Potassium Channel β Subunits, hKCNMB3 and hKCNMB4 (2000)

We present the cloning and characterization of two novel calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4, that are enriched in the testis and brain, respectively. We compare and contrast the steady state and kinetic properties of these beta subunits with the previously cloned mouse beta1 (mKCNMB1) and the human beta2 subunit (hKCNMB2). Once inactivation is removed, we find that hKCNMB2 has properties similar to mKCNMB1. hKCNMB2 slows Hslo1 channel gating and shifts the current-voltage relationship to more negative potentials. hKCNMB3 and hKCNMB4 have distinct effects on slo currents not observed with mKCNMB1 and hKCNMB2. Although we found that hKCNMB3 does interact with Hslo channels, its effects on Hslo1 channel properties were slight, increasing Hslo1 activation rates. In contrast, hKCNMB4 slows Hslo1 gating kinetics, and modulates the apparent calcium sensitivity of Hslo1. We found that the different effects of the beta subunits on some Hslo1 channel properties are calcium-dependent. mKCNMB1 and hKCNMB2 slow activation at 1 microM but not at 10 microM free calcium concentrations. hKCNMB4 decreases Hslo1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. These results suggest that beta subunits in diverse tissue types fine-tune slo channel properties to the needs of a particular cell.
Neural roles for heme oxygenase: Contrasts to nitric oxide synthase (2001)

The heme oxygenase (HO) and nitric oxide (NO) synthase (NOS) systems display notable similarities as well as differences. HO and NOS are both oxidative enzymes using NADPH as an electron donor. The constitutive forms of the enzyme are differentially activated, with calcium entry stimulating NOS by binding to calmodulin, whereas calcium entry activates protein kinase C to phosphorylate and activate HO2. Although both NO and carbon monoxide (CO) stimulate soluble guanylyl cyclase to form cGMP, NO also S-nitrosylates selected protein targets. Both involve constitutive and inducible biosynthetic enzymes. However, functions of the inducible forms are virtual opposites. Macrophage-inducible NOS generates NO to kill other cells, whereas HO1 generates bilirubin to exert antioxidant cytoprotective effects and also provides cytoprotection by facilitating iron extrusion from cells. The neuronal form of HO, HO2, is also cytoprotective. Normally, neural NO in the brain seems to exert some sort of behavioral inhibition. However, excess release of NO in response to glutamate's N-methyl-d-aspartate receptor activation leads to stroke damage. On the other hand, massive neuronal firing during a stroke presumably activates HO2, leading to neuroprotective actions of bilirubin. Loss of this neuroprotection after HO inhibition by mutant forms of amyloid precursor protein may mediate neurotoxicity in Familial Alzheimer's Disease. NO and CO both appear to be neurotransmitters in the brain and peripheral autonomic nervous system. They also are physiologic endothelial-derived relaxing factors for blood vessels. In the gastrointestinal pathway, NO and CO appear to function as coneurotransmitters, both stimulating soluble guanylyl cyclase to cause smooth muscle relaxation.
Correcting errors in synthetic DNA through consensus shuffling (2005)

Although efficient methods exist to assemble synthetic oligonucleotides into genes and genomes, these suffer from the presence of 1-3 random errors/kb of DNA. Here, we introduce a new method termed consensus shuffling and demonstrate its use to significantly reduce random errors in synthetic DNA. In this method, errors are revealed as mismatches by re-hybridization of the population. The DNA is fragmented, and mismatched fragments are removed upon binding to an immobilized mismatch binding protein (MutS). PCR assembly of the remaining fragments yields a new population of full-length sequences enriched for the consensus sequence of the input population. We show that two iterations of consensus shuffling improved a population of synthetic green fluorescent protein (GFPuv) clones from approximately 60 to >90% fluorescent, and decreased errors 3.5- to 4.3-fold to final values of approximately 1 error per 3500 bp. In addition, two iterations of consensus shuffling corrected a population of GFPuv clones where all members were non-functional, to a population where 82% of clones were fluorescent. Consensus shuffling should facilitate the rapid and accurate synthesis of long DNA sequences.
Activation of Intestinal Mucosal Immunity in Tumor‐bearing Mice by Lactoferrin (2000)

We have previously demonstrated that oral administration of bovine lactoferrin (bLF) markedly increases CD4(+) and CD8(+) T cells and NK (asialoGM1(+) ) cells in the blood of tumor-bearing mice and enhances anti-metastatic activity. In this paper, we document that oral administration of bLF and bLF-hydrolysate (bLFH) is associated with strong increases in CD4(+) and CD8(+) T, as well as asialoGM1(+) cells in lymphoid tissues and lamina propria of the small intestine in mice, especially in tumor-bearing animals in which Co26Lu cells were implanted subcutaneously. Moreover, IgM(+) and IgA(+) B cells in lamina propria of the small intestine were also significantly increased by bLF and bLFH. Bovine apo-transferrin (bTF) did not exhibit such activity. In the colon, only CD8(+) cells were significantly increased by treatment with bLF, while asialoGM1(+) cells were significantly decreased. bLF and bLFH induced cytokines to activate T, B and asialoGM1(+) cells. Administration of bLF and bLFH, but not bTF, increased production of interleukin-18 (IL-18), interferon-gamma (IFN-gamma) and caspase-1 in the mucosa of the small intestine. Particularly high levels of IL-18 were found in the epithelial cells of the small intestine. Moreover, administration of bLF and bLFH, but not bTF, induced IFN-gamma presenting cells in the small intestine. Caspase-1, which processes proIL-18 to mature IL-18, was also induced in the epithelial cells of the small intestine following treatment with bLF and bLFH, but not with bTF. These results suggest that enhanced production of IL-18 and IFN-gamma and caspase-1 induction by treatment with bLF may be important for elevation of intestinal mucosal immunity.
The perils of inclusion: the Constitution and the race power (1997)

onstitutionally speaking, Australia has been described as frozen continent.' Yet on 27 May 1967 the of Australia demonstrated an historic consensus in their desire to remove a blight on the Con~titution.~ Their decision to amend s5l(xxvi) and strike out s127 of the Constitution was of profound importance to both Aboriginal and non-Aboriginal Australians. The constitutional ramifications of this are still to be worked out. In the current climate, where the issue of the extinguishment of native title has generated considerable political heat, the question of the scope of the Commonwealth Parliament's power to make special laws for people of any race
Molecular interactions between Vestigial and Scalloped promote wing formation in Drosophila (1998)

Scalloped (Sd) and Vestigial (Vg) are each needed for Drosophila wing development. We show that Sd is required for Vg function and that altering their relative cellular levels inhibits wing formation. In vitro, Vg binds directly to both Sd and its human homolog, Transcription Enhancer Factor-1. The interaction domains map to a small region of Vg that is essential for Vg-mediated gene activation and to the carboxy-terminal half of Sd. Our observations indicate that Vg and Sd function coordinately to control the expression of genes required for wing development, which implies that Vg is a tissue-specific transcriptional intermediary factor of Sd.
A network organization used for document retrieval (1983)

A network organization for implementing a document retrieval system is proposed. This organization has significant advantages in terms of the range of searches that can be used when compared to either inverted or clustered file organizations. Algorithms for generating and maintaining the network are described together with experiments designed to test their efficiency and effectiveness.
The effects of cannabidiol and its synergism with bortezomib in multiple myeloma cell lines. A role for transient receptor potential vanilloid type‐2 (2013)

Multiple myeloma (MM) is a plasma cell (PC) malignancy characterised by the accumulation of a monoclonal PC population in the bone marrow (BM). Cannabidiol (CBD) is a non-psychoactive cannabinoid with antitumoural activities, and the transient receptor potential vanilloid type-2 (TRPV2) channel has been reported as a potential CBD receptor. TRPV2 activation by CBD decreases proliferation and increases susceptibility to drug-induced cell death in human cancer cells. However, no functional role has been ascribed to CBD and TRPV2 in MM. In this study, we identified the presence of heterogeneous CD138+TRPV2+ and CD138+TRPV2- PC populations in MM patients, whereas only the CD138+ TRPV2- population was present in RPMI8226 and U266 MM cell lines. Because bortezomib (BORT) is commonly used in MM treatment, we investigated the effects of CBD and BORT in CD138+TRPV2- MM cells and in MM cell lines transfected with TRPV2 (CD138+TRPV2+). These results showed that CBD by itself or in synergy with BORT strongly inhibited growth, arrested cell cycle progression and induced MM cells death by regulating the ERK, AKT and NF-κB pathways with major effects in TRPV2+ cells. These data provide a rationale for using CBD to increase the activity of proteasome inhibitors in MM.
Guanine nucleotide-binding protein regulation of melatonin receptors in lizard brain. (1989)

Melatonin receptors were identified and characterized in crude membrane preparations from lizard brain by using 125I-labeled melatonin (125I-Mel), a potent melatonin agonist. 125I-Mel binding sites were saturable; Scatchard analysis revealed high-affinity and lower affinity binding sites, with apparent Kd of 2.3 +/- 1.0 x 10(-11) M and 2.06 +/- 0.43 x 10(-10) M, respectively. Binding was reversible and inhibited by melatonin and closely related analogs but not by serotonin or norepinephrine. Treatment of crude membranes with the nonhydrolyzable GTP analog guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), significantly reduced the number of high-affinity receptors and increased the dissociation rate of 125I-Mel from its receptor. Furthermore, GTP[gamma S] treatment of ligand-receptor complexes solubilized by Triton X-100 also led to a rapid dissociation of 125I-Mel from solubilized ligand-receptor complexes. Gel filtration chromatography of solubilized ligand-receptor complexes revealed two major peaks of radio-activity corresponding to Mr greater than 400,000 and Mr ca. 110,000. This elution profile was markedly altered by pretreatment with GTP[gamma S] before solubilization; only the Mr 110,000 peak was present in GTP[gamma S]-pretreated membranes. The results strongly suggest that 125I-Mel binding sites in lizard brain are melatonin receptors, with agonist-promoted guanine nucleotide-binding protein (G protein) coupling and that the apparent molecular size of receptors uncoupled from G proteins is about 110,000.