gebhart/patrepeval huggingface dataset:
Amino acid sequence and characterization of a protein inhibitor of protein kinase C. (1990)

The complete primary structure has been determined for an inhibitor protein of protein kinase C. The bovine brain-derived inhibitor has a pI of 6 and its N-terminal alanine residue is blocked by acetylation. Fragments obtained by chemical and enzymatic cleavage of the purified inhibitor were analyzed by Edman degradation, fast atom bombardment mass spectrometry, and tandem mass spectrometry. The results establish that the protein has a calculated average molecular mass of 13,690 daltons and contains 125 amino acid residues with the following sequence: (sequence: see text) The inhibitor does not show significant homology with any other known protein. Circular dichroism of the freshly prepared apoprotein indicated a secondary structural content of 23% alpha-helix, 31% beta-sheet, and 11% beta-turn. Immobilization on nitrocellulose followed by exposure to a 65Zn2(+)-containing overlay solution showed that, like protein kinase C itself, the inhibitor is a zinc-binding protein, although the sequence does not reveal a "zinc finger" structure. Competition with 10-fold molar excess Ca2+ or Mg2+ did not reduce the zinc-binding specificity of this inhibitor.
Role of hydrophobic interactions and desolvation in determining the structural properties of a model alpha beta peptide. (1996)

Model AB, a 20-amino acid peptide that was designed to adopt an alpha beta tertiary structure stabilized by hydrophobic interactions between residues in adjacent helical and extended segments, exhibited large pKa shifts of several ionizable groups and slow hydrogen/deuterium exchange rates of nearly all the peptide amide groups [Butcher, D. J., Bruch, M. D. & Moe, G. T. (1995) Biopolymers 36, 109-120]. These properties, which depend on structure and hydration, are commonly observed in larger proteins but are quite unusual for small peptides. To identify which of several possible features of the peptide design are most important in determining these properties, several closely related analogs of Model AB were characterized by CD and NMR spectroscopy. The results show that hydrophobic interactions between adjacent helical and extended segments are structure-determining and have the additional effect of altering water-peptide interactions over much of the peptide surface. These results may have important implications for understanding mechanisms of protein folding and for the design of independently folding peptides.
Massive hepatic fibrosis in Gaucher's disease: clinico-pathological and radiological features (2000)

Hepatomegaly is frequent in patients with type 1 Gaucher's disease and is associated with infiltration of the liver with pathological macrophages. Most patients suffer no significant clinical consequences, but a few develop portal hypertension which may progress to parenchymal liver failure. We describe four patients with Gaucher's disease who have developed portal hypertension. We have reviewed their clinical histories and all available histological and radiological material. All had severe Gaucher's disease with multi-organ involvement, and had undergone splenectomy in childhood. Histologically, this advanced liver disease was characterized by a picture of extreme and advanced confluent fibrosis occupying the central region of the liver. This massive fibrosis is associated with characteristic radiological appearances. The liver histology in these cases is highly unusual and virtually unknown in other conditions. Our studies indicate that without specific treatment the liver disease is progressive and rapidly fatal. However, institution of enzyme replacement therapy with imiglucerase may have beneficial effects even when the condition is far advanced.
Characterization of Antibody Responses Elicited by Human Immunodeficiency Virus Type 1 Primary Isolate Trimeric and Monomeric Envelope Glycoproteins in Selected Adjuvants (2006)

We previously reported that soluble, stable YU2 gp140 trimeric human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein immunogens could elicit improved breadth of neutralization against HIV-1 isolates compared to monomeric YU2 gp120 proteins. In this guinea pig immunization study, we sought to extend these data and determine if adjuvant could quantitatively or qualitatively alter the neutralizing response elicited by trimeric or monomeric immunogens. Consistent with our earlier studies, the YU2 gp140 immunogens elicited higher-titer neutralizing antibodies against homologous and heterologous isolates than those elicited by monomeric YU2 gp120. Additionally, the GlaxoSmithKline family of adjuvants AS01B, AS02A, and AS03 induced higher levels of neutralizing antibodies compared to emulsification of the same immunogens in Ribi adjuvant. Further analysis of vaccine sera indicated that homologous virus neutralization was not mediated by antibodies to the V3 loop, although V3 loop-directed neutralization could be detected for some heterologous isolates. In most gp120-inoculated animals, the homologous YU2 neutralization activity was inhibited by a peptide derived from the YU2 V1 loop, whereas the neutralizing activity elicited by YU2 gp140 trimers was much less sensitive to V1 peptide inhibition. Consistent with a less V1-focused antibody response, sera from the gp140-immunized animals more efficiently neutralized heterologous HIV-1 isolates, as determined by two distinct neutralization formats. Thus, there appear to be qualitative differences in the neutralizing antibody response elicited by YU2 gp140 compared to YU2 monomeric gp120. Further mapping analysis of more conserved regions of gp120/gp41 may be required to determine the neutralizing specificity elicited by the trimeric immunogens.
Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae. (1989)

Changes in the dosage of genes encoding elongation factor EF-1 alpha were shown to cause parallel changes in the misreading of nonsense codons. Higher amounts of EF-1 alpha were correlated with increased nonsense suppression, suggesting that the level of EF-1 alpha is critically involved in translational fidelity.
Thermometry considerations in localized hyperthermia (1978)

The introduction of local hyperthermia as a method of cancer therapy implies the necessity of quantitative measurements of the thermal dose. Our intention is to describe the nature of the problem, both physically and physiologically, with illustrations drawn from thermographic measurements in phantoms and in animals. The characteristics of a thermometry calibration facility are described. Some measurement problems associated with conventional thermometer probes are mentioned and several new thermometers which were developed for use in the electromagnetic fields are reviewed. We present some of the concepts that will guide the development of noninvasive thermometry. Systemic hyperthermia is not considered. We recommend that other reviews specifically directed toward localized hyperthermia be prepared on the methods of heating and on thermal physiological problems.
Synteny-defined candidate genes for congenital and idiopathic scoliosis (1999)

Idiopathic scoliosis (IS) is a common but poorly understood syndrome. Congenital scoliosis (CS) is less common but comparably unexplored. Previous studies suggest that each has a significant genetic component. However, the occurrence of scoliosis in the presence of other hereditary connective tissue syndromes raises the possibility that IS and CS are in fact a heterogeneous group of disorders with varied pathogenetic mechanisms. Mouse mutations have proven informative in identifying genes that are important in the development of the musculoskeletal system and provided important mechanistic insights regarding their roles in human disease. We sought to identify candidate genes for human IS and CS by reviewing mouse mutations with phenotypes affecting the axial skeleton. We performed a systematic review using the Mouse Genome Database (MGD), the Genome Database (GDB), and the Online Mendelian Inheritance in Man (OMIM) world-wide-web sites with additional searches performed based on the results of this initial search. We identified approximately 400 mouse mutations, reviewed approximately 250 of these for vertebral phenotypes, assessed 45 of these for synteny conservation between mouse and man, and identified 28 mouse mutations for which 29 credible candidates for human scoliosis could be identified based on mouse phenotypic and mapping data. For each of these, we have synthesized information about the mouse mutant phenotype, mapping data, information regarding molecular pathogenesis when a specific causative gene has been identified, and information regarding plausible candidates based on map position when the causative gene has not been identified. Among these were three loci for which the mutant gene had been identified and the human homologue was known. Some of the mouse mutants have phenotypes similar to human syndromes.
MOSFETs in laser-recrystallized poly-silicon on quartz (1980)

MOSFETs have been fabricated in layers of laser-recrystallized poly-crystalline silicon on bulk, amorphous, fused quartz substrates, with the silicon film formed into device islands either before or after laser recrystallization. Field-effect mobilities as high as 320 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /V-sec have been obtained. The recrystallization is influenced by the different light absorption in the transparent substrate and in the absorbing silicon film.
Recombinant protein protocols: detection and isolation. (1997)

A major success story of modem molecular biology is the development of technologies to clone and express specific genes. Current applications of recombinant gene products cover a wide spectrum, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, agriculture and animal husbandry, and so on. Inherent in bringing these appli­ cations to fruition is the need to design "expression constructs" that will per­ mit the ready and specific detection and isolation of the defined recombinant gene products. Recombinant Protein Protocols grows out of the need for a laboratory manual on the detection and isolation of recombinantly expressed genes that covers both the background information and the practical laboratory recipes for these analyses. In this book, detailed and contemporary protocols are col­ lected to provide the reader with a wide-ranging number of methodologies to enhance the detection and isolation of their gene product(s) of interest. A large number of molecular tags and labels and their usage are described, including enzymes, ligand-binding moieties, immunodetectable molecules, as well as methods to detect interactive proteins, and gene expression-mediated alter­ ations in cellular activity. Chapters on in situ detection of gene expression deal with technologies that are currently being applied to the study of gene function and activity. Highlights of applications for recombinant gene expres­ sion technologies are provided to give readers exciting perspectives on the future of such technologies
Current understanding and management of dilated cardiomyopathy in Duchenne and Becker muscular dystrophy (2009)

Implementing a routine cardiac assessment and timely pharmacological treatment in primary or specialty care settings is highlighted as an important step to optimize cardiac health among patients with Duchenne and Becker muscular dystrophies.
REVIEWS (1973)

Journal Article REVIEWS Get access L'Évangile selon Matthieu: rédaction et théologie. Edited by M. DIDIER. Pp. 428. (Bibliotheca Ephemeridum Theologicarum Lovaniensium, XXIX.) Gembloux: Éditions J. Duculot, 1972. FB. 750. H. BENEDICT GREEN H. BENEDICT GREEN Search for other works by this author on: Oxford Academic Google Scholar The Journal of Theological Studies, Volume XXIV, Issue 1, April 1973, Pages 224–225, https://doi.org/10.1093/jts/XXIV.1.224 Published: 01 April 1973
Improved Newton–Raphson digital image correlation method for full-field displacement and strain calculation (2010)

The two-dimensional in-plane displacement and strain calculation problem through digital image processing methods has been studied extensively in the past three decades. Out of the various algorithms developed, the Newton-Raphson partial differential correction method performs the best quality wise and is the most widely used in practical applications despite its higher computational cost. The work presented in this paper improves the original algorithm by including adaptive spatial regularization in the minimization process used to obtain the motion data. Results indicate improvements in the strain accuracy for both small and large strains. The improvements become even more significant when employing small displacement and strain window sizes, making the new method highly suitable for situations where the underlying strain data presents both slow and fast spatial variations or contains highly localized discontinuities.
Warfarin Sodium versus Low-Dose Heparin in the Long-Term Treatment of Venous Thrombosis (1979)

Acute deep-vein thrombosis is usually treated with intravenous heparin for a number of days, then with oral anticoagulants for weeks to months. We have compared adjusted-dose warfarin sodium with fixed low-dose subcutaneous heparin in the prevention of recurrent deep-vein thrombosis. Sixty-eight patients with acute deep-vein thrombosis confirmed by venography were treated with intravenous heparin and then randomized to secondary prophylaxis. Nine of 35 patients receiving subcutaneous heparin, but none of 33 receiving warfarin sodium, had new episodes of objectively documented venous thromboembolism (P = 0.001). Seven patients on warfarin sodium experienced bleeding complications (of which four were major), as compared with no patients receiving subcutaneous heparin (P less than 0.005). Thus, adjusted-dose warfarin sodium is more effective than low-dose subcutaneous heparin in preventing recurrent venous thromboembolism, but its use is accompanied by a significant risk of bleeding.
Properties of a hollow-core photonic bandgap fiber at 850 nm wavelength (2003)

We describe a hollow-core photonic bandgap fiber designed for use in the 850 nm wavelength region. The fiber has a minimum attenuation of 180dB/km at 847nm wavelength. The low-loss mode has a quasi- Gaussian intensity profile. The group-velocity dispersion of this mode passes through zero around 830nm, and is anomalous for longer wavelengths. The polarization beat length varies from 4 mm to 13 mm across the band gap. We expect this fiber to be useful for delivery of high-energy ultrashort optical pulses.
Overview of osteoporosis. (1991)

Osteoporosis is a common age-related disorder manifested clinically by skeletal fractures, especially fractures of the vertebrae, hip, and distal forearm. The major cause of these fractures is low bone mass, although an increase in trauma due to falls in the elderly also contributes. There are multiple causes for the low bone mass which, in any given individual, may contribute differently to the development of the osteopenia. The most important groups of causes are failure to achieve adequate peak bone mass, slow bone loss due to processes relating to aging, the menopause in women, and a variety of sporadic behavioral, nutritional, and environmental factors that affect bone mass in some but not in other individuals. The most important approach is prevention. Drugs and behavioral factors known to cause bone loss should be eliminated and perimenopausal women should be evaluated for possible preventive administration of estrogen. For patients with fractures due to established osteoporosis, the only drugs approved by the Food and Drug Administration are the antiresorptive agents calcium, estrogen, and calcitonin. Formation-stimulating regimens, however, are being developed and may be available for clinical use in the foreseeable future. These regimens may be capable of increasing bone mass to above the fracture threshold, thereby resulting in a clinical cure of the osteoporosis.
Antibody-Mediated Clearance of Amyloid-β Peptide from Cerebral Amyloid Angiopathy Revealed by QuantitativeIn VivoImaging (2007)

Cerebral amyloid angiopathy (CAA) is the accumulation of amyloid-beta peptide (Abeta) in the vessel wall of arteries in the brain. Because CAA is commonly associated with Alzheimer's disease (AD), characterized by parenchymal deposition of the same peptide in the form of senile plaques, there is considerable interest in the relationship of the two deposits in generating human disease. The study of CAA is of particular importance for immunotherapeutic approaches to AD, because reports of anti-Abeta immunotherapy in mice and humans have suggested that, whereas CAA appeared resistant to clearance, its response to this treatment promoted potential adverse effects, including meningoencephalitis. We used multiphoton microscopy and longitudinal imaging to monitor CAA in a mouse model of amyloid deposition to evaluate the effects of anti-Abeta passive immunotherapy. We found detectable clearance of CAA deposits within 1 week after a single administration of antibody directly to the brain, an effect that was short-lived. Chronic administration of antibody over 2 weeks led to more robust clearance without evidence of hemorrhage or other destructive changes. We found that the progressive clearance of Abeta from vessels follows distinct kinetics from what has been previously reported for clearance of plaques (parenchymal deposits of Abeta). This quantitative in vivo imaging approach directly demonstrates that CAA in a transgenic mouse model can be cleared with an optimized immunotherapy.
Clues to the Pathogenesis of Familial Colorectal Cancer (1993)

A predisposition to colorectal cancer is shown to be linked to markers on chromosome 2 in some families. Molecular features of "familial" cancers were compared with those of sporadic colon cancers. Neither the familial nor sporadic cancers showed loss of heterozygosity for chromosome 2 markers, and the incidence of mutations in KRAS, P53, and APC was similar in the two groups of tumors. Most of the familial cancers, however, had widespread alterations in short repeated DNA sequences, suggesting that numerous replication errors had occurred during tumor development. Thirteen percent of sporadic cancers had identical abnormalities and these cancers shared biologic properties with the familial cases. These data suggest a mechanism for familial tumorigenesis different from that mediated by classic tumor suppressor genes.
Polyphenolic Constituents of Mαgnoliα pterocαrpα Roxb. (1983)

Department of Chemistry, University College of Science, Calcutta.-700 009 <em>Manuscript received 12 March 1982, accepted 12 November 1982</em> Polyphenolic Constituents of <em>Mαgnoliα pterocαrpα</em> Roxb.
Dietary supplementation of n‐3 PUFA reduces weight gain and improves postprandial lipaemia and the associated inflammatory response in the obese JCR:LA‐cp rat (2009)

Acute dietary n-3 PUFA dietary supplementation can improve fasting as well as postprandial lipid metabolism and components of the associated inflammatory response in the JCR:LA-cp rat. Further, moderate dose n-3 PUFA supplementation may reduce corresponding body weight during conditions of hypercholesterolaemia and/or modulate inflammation associated with obesity and the metabolic syndrome.
Lymphatic uptake of lipid nanoparticles following endotracheal administration (2006)

A previous publication reported the uptake into the lymphatics of pulmonary administered lipid nanoparticles (LN), after aerosolization and inhalation. In the present study LN clearance from the lungs and lymphatic uptake were further evaluated after endotracheal administration. Nanoparticles prepared with gliceryl behenate were radiolabelled by association to the lipophilic tracer D,L-hexamethylpropylene amine oxime (HMPAO) coupled with 99mTc. Labelling efficiency was 97% and stability in body fluids was demonstrated in vitro. Wistar rats were treated by endotracheal administration and lymphatic uptake was determined upon organ sampling. Endotracheally delivered LN are rapidly eliminated from rat lungs and accumulation in para-aortic, axillary and inguinal lymph nodes starts almost immediately after administration. Translocation of LN across the lung mucosa and their uptake into the lymphatics demonstrate their usefulness as potential drug carriers for lung cancer therapy, as well as for immunization purposes.
Particle Size Magnifier for Nano-CN Detection (2011)

A new particle size magnifier (PSM) for detection of nano-CN as small as 1 nm in mobility diameter was developed, calibrated and tested in atmospheric measurements.The working principle of a PSM is to mix turbulently cooled sample flow with heated clean air flow saturated by the working fluid.This provides a high saturation ratio for the working fluid and activates the seed particles and grows them by condensation of the working fluid.In order to reach high saturation ratios, and thus to activate nano-CN without homogeneous nucleation, diethylene glycol was chosen as the working fluid.The PSM was able to grow nano-CN to mean diameter of 90 nm, after which an ordinary condensation particle counter was used to count the grown particles (TSI 3010).The stability of the PSM was found to be good making it suitable for stand-alone field measurements.Calibration results show that the detection efficiency of the prototype PSM + TSI 3010 for charged tetra-alkyl ammonium salt molecules having mobility equivalent diameters of 1.05, 1.47, 1.78, and 2.57 nm are 25, 32, 46, and 70%, respectively.The commercial version of the PSM (Airmodus A09) performed even better in the smallest sizes the detection efficiency being 51% for 1.47 nm and 67% for 1.78 nm.
An SOI-based three-dimensional integrated circuit technology (2002)

Three-dimensional integrated circuits (3D-ICs) composed of active circuit layers that are vertically stacked and interconnected are expected to lead to improved logic devices, memories, CPUs, and photosensors (Akasaka, 1986). These circuits require high-density vertical interconnections (3D vias) comparable in aspect ratio to present multilevel vias (Reber and Tielert, 1996). We have constructed and tested 3D ring oscillators and fully parallel 64/spl times/64 active pixel sensors using a 3D assembly technology which utilizes SOI wafers to achieve stacking of multiple circuit layers and unrestricted placement of dense 3D vias.
Biometric identification via an oculomotor plant mathematical model (2010)

There has been increased interest in reliable, non-intrusive methods of biometric identification due to the growing emphasis on security and increasing prevalence of identity theft. This paper presents a new biometric approach that involves an estimation of the unique oculomotor plant (OP) or eye globe muscle parameters from an eye movement trace. These parameters model individual properties of the human eye, including neuronal control signal, series elasticity, length tension, force velocity, and active tension. These properties can be estimated for each extraocular muscle, and have been shown to differ between individuals. We describe the algorithms used in our approach and the results of an experiment with 41 human subjects tracking a jumping dot on a screen. Our results show improvement over existing eye movement biometric identification methods. The technique of using Oculomotor Plant Mathematical Model (OPMM) parameters to model the individual eye provides a number of advantages for biometric identification: it includes both behavioral and physiological human attributes, is difficult to counterfeit, non-intrusive, and could easily be incorporated into existing biometric systems to provide an extra layer of security.