gebhart/patrepeval huggingface dataset:
Suppression of early atherosclerosis in LDL-receptor deficient mice by oral tolerance with $beta;2-glycoprotein I (2004)

Background: Atherosclerosis is considered analogous to chronic inflammatory diseases. Beta2-glycoprotein I (β2GPI) is a phospholipid binding protein shown to serve as a target for prothrombotic antiphospholipid antibodies. It has recently been demonstrated to drive an immune mediated reaction and enhance murine atherosclerosis. Oral tolerance is a method in which feeding a given antigen, downregulates the respective immune responses towards it, and attenuates concomitant organ specific disorders. Herein, we tested the hypothesis, that inhibiting cellular immunity to β2GPI would result in suppression of fatty streak formation in mice. Methods and results: LDL receptor deficient mice were fed different doses of human or bovine β2GPI or BSA and than switched to an atherogenic diet. To determine the effect of feeding on lymph node proliferative indices, separate groups of mice were fed β2GPI and then immunized with the respective antigen. Feeding either human or bovine β2GPI was effective in attenuating atherosclerosis as compared to control fed animals. Oral feeding with of β2GPI inhibited lymph node cell reactivity to β2GPI in mice immunized against the human protein. Oral tolerance was also capable of reducing reactivity to oxidized LDL in mice immunized against oxLDL. IL-4 and IL-10 production was upregulated in lymph node cells of β2GPI-tolerant mice immunized against β2GPI, upon priming with the respective protein. Conclusion: Thus, oral administration of β2GPI is an effective means of suppressing atherogenesis in mice and should further be investigated.
Polyswitch Lentivectors: “All-in-One” Lentiviral Vectors for Drug-Inducible Gene Expression, Live Selection, and Recombination Cloning (2011)

Lentiviral vectors are now widely considered one of the safest and most efficient tools for gene delivery and stable gene expression. Even though inducible gene expression cassettes are mandatory for many genetic engineering strategies, most current systems suffer from various issues, such as the requirement of two vectors, which decreases the overall efficiency of the transduction, leakiness and/or insufficient levels of activation of the inducible promoter, lack of selectable marker, low titers, or general issues associated with the cloning of large plasmids. In this article, we describe the design and functional characterization of a set of "all-in-one" multicistronic autoinducible lentivectors. They combine: (1) an optimized drug-inducible promoter; (2) a multicistronic strategy to express living color, selectable marker, and transactivator; and (3) acceptor sites for easy recombination cloning of genes of interest. These polyswitch lentivectors have good titers, very low basal activity, and reversible high induced activity, and can accept a growing number of genes already cloned in entry plasmids. These combined features make them a novel, powerful, and versatile tool for current and future genetic engineering approaches.
Contact impedance estimation for robotic systems (2005)

In this paper, the problem of online estimation of the mechanical impedance during the contact of a robotic system with an unknown environment is considered. This problem is of great interest when controlling a robot in an unstructured and unknown environment, such as in telemanipulation tasks, since it can be easily shown that the exploitation of the knowledge of the mechanical properties of the environment can greatly improve the performance of the robotic system. In particular, a single-point contact is considered, and the (nonlinear) Hunt-Crossley model is taken into account, instead of the classical (linear) Kelvin-Voigt model. Indeed, the former achieves a better physical consistency and also allows describing the behavior of soft materials. Finally, the online estimation algorithm is described and experimental results are presented and discussed.
High-Efficiency Fluorescent Blue Organic Light-Emitting Device with Balanced Carrier Transport (2007)

We demonstrate a blue fluorescent organic light-emitting device having a current efficiency of and an external quantum efficiency of 8.32% at . While driving at , the device showed a current efficiency of with a CIE coordinate at (0.155, 0.239) and an operation voltage of . The estimated half-decay lifetime is at an initial luminance of . The improved performance is the result of better carrier balance which is achieved by using bis(10-hydroxybenzo[h]quinolinato)beryllium as the electron-transport layer.
Organometallic Catalysis in Aqueous Solution. The Hydrolytic Activity of a Water-Soluble ansa-Molybdocene Catalyst (2007)

The synthesis, characterization, and reactivity of the new water-soluble ansa-molybdocene catalyst [{C2Me4(C5H4)2}Mo(OH)(OH2)][OTs] (3) and the related hydroxo-bridged dimer [{C2Me4(C5H4)2}Mo(μ-OH)]2[OTs]2 (5) are described. The effect of the ethylene bridge on the metallocene structure was evaluated by comparing the crystal structures of {C2Me4(C5H4)2}MoH2 (2) and 5 to those of the non-ansa analogues. The ethylene bridge changed the bite angles of the metallocene fragment by only a few degrees in both ansa structures. To probe the electronic consequences of the tetramethylethylene bridge, the {C2Me4(C5H4)2}Mo(CO)H (4) complex was prepared. On the basis of the ν(C⋮O) stretching frequencies, the ansa ligand C2Me4(C5H4)2 was found to be electron-withdrawing relative to two η5-C5H5 ligands. The reactivity of 3 in nitrile hydration, phosphate ester hydrolysis, and carboxylic acid ester hydrolysis was explored, and the rate constants for these transformations were compared to rate constants obtained using the Cp2Mo(OH)(OH2)+ and Cp‘2Mo(OH)(OH2)+ catalysts. In all cases, the Cp2Mo(OH)(OH2)+ catalyst, having intermediate electron density, had the largest rate constants. The reactivity trends for the three catalysts are explained by the relative electrophilicities of the Mo centers. If electron-donating cyclopentadienyl ligands are employed, the reactivity of the bound substrate is decreased relative to Cp and the rate is decreased. Conversely, if electron-withdrawing Cp cyclopentadienyl ligands are employed, the reactivity of the bound hydroxo nucleophile is decreased and the rate is decreased. In the case of the Cp2Mo(OH)(OH2)+ complex, these two opposing trends converge, and optimal activity is observed.
The Armenian Church under the Soviet and independent regimes, part 3: The leadership of Vazgen (1998)

(1998). The Armenian Church under the Soviet and independent regimes, part 3: The leadership of Vazgen. Religion, State and Society: Vol. 26, No. 3-4, pp. 291-355.
Hyaluronic acid through a new injectable nerve guide delivery system enhances peripheral nerve regeneration in the rat (1995)

The use of non-neural conduits to bridge gaps in peripheral nerves has been noted in the literature for many years. A logical extension of this concept is the introduction of neurotrophic or growth promoting factors into the lumen. We present here an injectable nerve guide that allows percutaneous access to the microenvironment of the regenerating peripheral nerve within the guide's lumen. Hyaluronic acid, a compound associated with decreased scarring and improved fibrin matrix formation, is added sequentially to the regenerating peripheral rat sciatic nerve via this injectable nerve guide. Assessment of nerve regeneration and reinnervation shows better conduction velocity, higher axon counts, and a trend toward earlier myelination with hyaluronic acid compared with saline. This work not only implies hyaluronic acid's role as an agent that aids nerve growth but also describes a new tool that allows percutaneous access to the milieu of a regenerating nerve.
Absolute Quantification of the Model Biomarker Prostate-Specific Antigen in Serum by LC−MS/MS Using Protein Cleavage and Isotope Dilution Mass Spectrometry (2004)

Protein cleavage-isotope dilution mass spectrometry (PC-IDMS) can be used to quantify proteins, with an isotope-labeled analogue of the peptide fragment used as an internal standard. Here, we investigate use of a standard LC-MS/MS platform for quantifying a model biomarker directly from serum by this technique. We synthesized a peptide (IVGGWECEK) identical to the N-terminal tryptic fragment of PSA but with each glycine containing two 13C atoms and one 15N atom. PSA-free human serum was denatured with urea followed by the introduction of PSA standard and the stable isotope labeled internal standard peptide. The sample was then proteolyzed with trypsin and subjected to quantification using LC-MS/ MS on a triple quadrupole mass spectrometer. A linear least squares calibration curve made from five different concentrations of PSA added to serum and digested (each made in triplicate and randomly injected three times) had a mean slope of 0.973 (SE = 0.023), intercept of -0.003 (SE = 0.022), and R2 of 0.971. Recovery of calibrators ranged from 70 to 85% with a mean run-to-run CV of 13% and a mean within-run CV of 5.7%. PC-IDMS is a promising technique for quantifying proteins covering a broad range of applications from standardizing immunoassays to monitoring post-translational modifications to quantifying newly discovered biomarkers prior to the development and implementation of an immunoassay, just to name a few. Issues surrounding the application of PC-IDMS for the absolute quantification of proteins include selection of a proteolytic fragment for quantification that can be cleaved and isolated reproducibly over a broad dynamic range, stable isotope labeled synthetic peptide standards that give consistent results, and LC-MS/MS methods that provide adequate sensitivity and reproducibility without creating impractical analysis times. The results presented here show that absolute quantification can be performed on the model biomarker PSA introduced into denatured serum when analyzed by LC-MS/MS. However, concerns still exist regarding sensitivity compared to existing immunoassays as well as the reproducibility of PC-IDMS performed in different matrixes.
Transforming Growth Factor (TGF)-β1–producing Regulatory T Cells Induce Smad-mediated Interleukin 10 Secretion That Facilitates Coordinated Immunoregulatory Activity and Amelioration of TGF-β1–mediated Fibrosis (2003)

Interleukin (IL)-10 and transforming growth factor (TGF)-beta1 are suppressor cytokines that frequently occur together during a regulatory T cell response. Here we used a one gene doxycycline (Dox)-inducible plasmid encoding TGF-beta1 to analyze this association and test its utility. In initial studies, we showed that intranasal administration of this plasmid (along with Dox) led to the appearance of TGF-beta1-producing cells (in spleen and lamina propria) and the almost concomitant appearance of IL-10-producing cells. Moreover, we showed that these cells exert Dox-regulated suppression of the T helper cell (Th)1-mediated inflammation in trinitrobenzene sulfonic acid colitis. In subsequent in vitro studies using retroviral TGF-beta1 expression, we established that IL-10 production by Th1 cells occurs after exposure to TGF-beta1 from either an endogenous or exogenous source. In addition, using a self-inactivating retrovirus luciferase reporter construct we showed that TGF-beta1 induces Smad4, which then binds to and activates the IL-10 promoter. Furthermore, intranasal TGF-beta1 plasmid administration ameliorates bleomycin-induced fibrosis in wild-type but not IL-10-deficient mice, strongly suggesting that the amelioration is IL-10 dependent and that IL-10 protects mice from TGF-beta1-mediated fibrosis. Taken together, these findings suggest that the induction of IL-10 by TGF-beta1 is not fortuitous, but instead fulfills important requirements of TGF-beta1 function after its secretion by regulatory T cells.
Weighted selection of image features for resolved rate visual feedback control (1991)

The authors develop methodologies for the automatic selection of image features to be used to visually control the relative position and orientation (pose) between the end-effector of an eye-in-hand robot and a workpiece. A resolved motion rate control scheme is used to update the robot's pose based on the position of three features in the camera's image. The selection of these three features depends on a blend of image recognition and control criteria. The image recognition criteria include feature robustness, completeness, cost of feature extraction, and feature uniqueness. The control criteria include system observability, controllability, and sensitivity. A weighted criteria function is used to select the combination of image features that provides the best control of the end-effector of a general six-degrees-of-freedom manipulator. Both computer simulations and laboratory experiments on a PUMA robot arm were conducted to verify the performance of the feature-selection criteria.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Near-Infrared Detection of Flow Injection Analysis by Acoustooptic Tunable Filter-Based Spectrophotometry (1996)

The instrumentation development of a near-infrared (near-IR) spectrophotometer based on an acoustooptic tunable filter (AOTF) and its application as a detector for flow injection analysis (FIA) are reported. In addition to being compact and all solid state, this AOTF-based instrument is very sensitive, has high resolution, and can be rapidly scanned. The latter advantage make it uniquely suited as a detector for FIA, in that it can rapidly record the whole near-IR absorption spectrum of a mixture passing through the FIA flow cell. Subsequent treatment of the recorded spectra with multivariate calibration methods makes it possible to use the FIA, for the first time, for such applications as the simultaneous determination of trace amounts of water and benzene in ethanol. Because all organic compounds absorb light in the near-infrared region, this AOTF-based near-IR detector can serve as a universal detector for FIA; as a consequence, applications of the FIA techniques can be expanded to other areas which are not possible otherwise.
Clearing the complexity: immune complexes and their treatment in lupus nephritis (2011)

Systemic lupus erythematosus (SLE) is a classic antibody-mediated systemic autoimmune disease characterised by the development of autoantibodies to ubiquitous self-antigens (such as antinuclear antibodies and antidouble-stranded DNA antibodies) and widespread deposition of immune complexes in affected tissues. Deposition of immune complexes in the kidney results in glomerular damage and occurs in all forms of lupus nephritis. The development of nephritis carries a poor prognosis and high risk of developing end-stage renal failure despite recent therapeutic advances. Here we review the role of DNA-anti-DNA immune complexes in the pathogenesis of lupus nephritis and possible new treatment strategies aimed at their control.
Microbiological safety of a novel bio-artificial liver support system based on porcine hepatocytes: a experimental study (2012)

No infective PERV was detected in the experimental animals, thus the novel BAL had a reliable microbiological safety profile.
Node-connection/quantum phase-shifting mask: Path to below 0.3 μm pitch, proximity effect free, random interconnects and memory patterning (1999)

New design concepts of the alternating phase-shifting masks (PSMs) are proposed. They enable alternating PSMs to be applied to random patterns that have the least design restrictions and reduced proximity effects. The original design patterns are decomposed into several subpatterns using geometrical operations, so that each subpatterns can be achieved by alternating type PSMs. The possibility of patterning a below 0.3 μm pitch random interconnect with conventional deep ultraviolet tools is shown.
Real-Time Robot Audition System That Recognizes Simultaneous Speech in The Real World (2006)

This paper presents a robot audition system that recognizes simultaneous speech in the real world by using robot-embedded microphones. We have previously reported missing feature theory (MFT) based integration of sound source separation (SSS) and automatic speech recognition (ASR) for building robust robot audition. We demonstrated that a MFT-based prototype system drastically improved the performance of speech recognition even when three speakers talked to a robot simultaneously. However, the prototype system had three problems; being offline, hand-tuning of system parameters, and failure in voice activity detection (VAD). To attain online processing, we introduced FlowDesigner-based architecture to integrate sound source localization (SSL), SSS and ASR. This architecture brings fast processing and easy implementation because it provides a simple framework of shared-object-based integration. To optimize the parameters, we developed genetic algorithm (GA) based parameter optimization, because it is difficult to build an analytical optimization model for mutually dependent system parameters. To improve VAD, we integrated new VAD based on a power spectrum and location of a sound source into the system, since conventional VAD relying only on power often fails due to low signal-to-noise ratio of simultaneous speech. We, then, constructed a robot audition system for Honda ASIMO. As a result, we showed that the system worked online and fast, and had a better performance in robustness and accuracy through experiments on recognition of simultaneous speech in a noisy and echoic environment
Unique potential of 4-1BB agonist antibody to promote durable regression of HPV + tumors when combined with an E6/E7 peptide vaccine (2015)

Antibody modulation of T-cell coinhibitory (e.g., CTLA-4) or costimulatory (e.g., 4-1BB) receptors promotes clinical responses to a variety of cancers. Therapeutic cancer vaccination, in contrast, has produced limited clinical benefit and no curative therapies. The E6 and E7 oncoproteins of human papilloma virus (HPV) drive the majority of genital cancers, and many oropharyngeal tumors. We discovered 15-19 amino acid peptides from HPV-16 E6/E7 for which induction of T-cell immunity correlates with disease-free survival in patients treated for high-grade cervical neoplasia. We report here that intranasal vaccination with these peptides and the adjuvant alpha-galactosylceramide elicits systemic and mucosal T-cell responses leading to reduced HPV(+) TC-1 tumor growth and prolonged survival in mice. We hypothesized that the inability of these T cells to fully reject established tumors resulted from suppression in the tumor microenvironment which could be ameliorated through checkpoint modulation. Combining this E6/E7 peptide vaccine with checkpoint blockade produced only modest benefit; however, coadministration with a 4-1BB agonist antibody promoted durable regression of established genital TC-1 tumors. Relative to other therapies tested, this combination of vaccine and α4-1BB promoted the highest CD8(+) versus regulatory FoxP3(+) T-cell ratios, elicited 2- to 5-fold higher infiltration by E7-specific CTL, and evoked higher densities of highly cytotoxic TcEO (T cytotoxic Eomesodermin) CD8 (>70-fold) and ThEO (T helper Eomesodermin) CD4 (>17-fold) T cells. These findings have immediate clinical relevance both in terms of the direct clinical utility of the vaccine studied and in illustrating the potential of 4-1BB antibody to convert therapeutic E6/E7 vaccines already in clinical trials into curative therapies.
Automatic assignment of soft Boolean operators (1985)

The conventional bibliographic retrieval systems are based on Boolean query formulations and inverted file implementations. Such systems provide rapid responses in answer to search queries but they are not easy to use by uninitiated patrons. An extended Boolean retrieval strategy has been devised in which the Boolean operators are treated more or less strictly, depending on the setting of a special parameter, known as the p-value. The extended system is much more forgiving than the conventional system, and provides better retrieval effectiveness. In this study various problems associated with the determination of appropriate p-values are discussed, and suggestions are made for an automatic assignment of p-values. Evaluation output is included to illustrate the operations of the suggested procedures.
Nicotinic receptor subtypes and cognitive function (2002)

Nicotinic receptor systems are involved in a wide variety of behavioral functions including cognitive function. Nicotinic medications may provide beneficial treatment for cognitive dysfunction such as Alzheimer's disease, schizophrenia, and attention deficit hyperactivity disorder (ADHD). Nicotine has been shown to improve attentional performance in all of these disorders. Better efficacy with fewer side effects might be achieved with novel nicotinic ligands selective for particular nicotinic subtypes. To develop these novel selective nicotinic ligands it is important to use animal models to determine the critical neurobehavioral bases for nicotinic involvement in cognitive function. Nicotine-induced cognitive improvement in rats is most consistently seen in working memory tasks. We have found that both acute and chronic nicotine administration significantly improves working memory performance of rats in the radial-arm maze. The pharmacologic and anatomic mechanisms for this effect have been examined in our laboratory in a series of local drug infusion studies. Both alpha 4 beta 2 and alpha 7 nicotinic receptors in the ventral hippocampus and basolateral amygdala are involved in working memory function. Working memory impairments were caused by local infusion of either alpha 4 beta 2 or alpha 7 antagonists. Ventral hippocampal alpha 4 beta 2 blockade-induced working memory deficits are reversed by chronic systemic nicotine treatment, while ventral hippocampal alpha 7 blockade-induced working memory deficits were not found to be reversed by the same nicotine regimen. Interestingly, alpha 4 beta 2 and alpha 7 induced deficits were not found to be additive in either the ventral hippocampus or the basolateral amygdala. In fact, in the amygdala, alpha 7 antagonist cotreatment actually reversed the working memory impairment caused by alpha 4 beta 2 antagonist administration. These studies of the neural nicotinic mechanisms underlying cognitive function are key for opening avenues for development of safe and effective nicotinic treatments for cognitive dysfunction.