gebhart/patrepeval huggingface dataset:
Orthogonal Ligation of Unprotected Peptide Segments through Pseudoproline Formation for the Synthesis of HIV-1 Protease Analogs, (1996)

We describe the total synthesis of three active HIV-1 protease analogs by an orthogonal ligation through thiaproline formation of two large, unprotected peptide segments. The central element of this strategy in achieving orthogonality of peptide bond formation is through proximity effect, and the key reaction is a side chain thiol initiated aldehyde capture to overcome the entropic problem of coupling between two large molecular weight peptide segments. The capture step also leads to specific entropic activation of the acyl segment because the respective termini are brought to close proximity, resulting in a spontaneous acyl rearrangement to form the amide bond. A general method using a thioester for introducing an aldehyde moiety to the C-terminus of an unprotected peptide segment was also developed. Three active analogs of HIV-1 protease were obtained in excellent yield by ligating two segments of 38 and 61 residues. Two analogs contained a thioproline residue at position 39, and the third contained a 38−39 non-peptide backbone. Efficient ligation at pH 4 was attained at peptide segment concentrations as low as 50 μM, a concentration which is not feasible with conventional convergent methods using protected peptide segments.
Decomposition of gaseous dielectrics (CF4,SF6) by a non-equilibrium plasma. Mechanisms, kinetics, mass spectrometric studies and interactions with polymeric targets (1994)

Abstract
Chemical and biological properties of some oxindolidyl-3-methines (1968)

Several 1-methyloxindohdyl-3-methines have been synthesized, either by condensation of 1-methyloxindole with an aldehyde, or by a Wittig reaction of a chloromethylene compound with a 1-methylisatin When examined m the mass spectrometer the expected ion reactions were observed and, in addition, a reaction involving expulsion of the carbonyl group of the oxindole moiety and rearrangement of the residual fragment, to give a 1-methylbenzo[b]azepinium ion was observed. The anions of the oxindolidyluracilmethines 4 and 5, (R = Me, R′ = OH) had an absorption band near 380 mμ but the corresponding neutral molecules absorbed at 325–350 mμ. The 5-nitrofuran derivatives 3 were the only compounds that inhibited the growth of Bacillus subtilis.
Automated parallel high-speed atomic force microscopy (1998)

An expandable system has been developed to operate multiple probes for the atomic force microscope in parallel at high speeds. The combined improvements from parallelism and enhanced tip speed in this system represent an increase in throughput by over two orders of magnitude. A modular cantilever design has been replicated to produce an array of 50 cantilevers with a 200 μm pitch. This design contains a dedicated integrated sensor and integrated actuator where the cells can be repeated indefinitely. Electrical shielding within the array virtually eliminates coupling between the actuators and sensors. The reduced coupling simplifies the control electronics, facilitating the design of a computer system to automate the parallel high-speed arrays. This automated system has been applied to four cantilevers within the array of 50 cantilevers, with a 20 kHz bandwidth and a noise level of less than 50 Å. For typical samples, this bandwidth allows us to scan the probes at 4 mm/s.
Teratocarcinomas and Mammalian Embryogenesis (1980)

In the last decade there has emerged an appreciation of the remarkable similarity between the cells that give rise to teratocarcinomas in mice and the cells that give rise to the developing mouse embryo. The resemblance is so close that in certain instances the tumor stem cells can join with their embryonic counterparts and develop into a completely normal mouse. The availability of stem cell lines isolated from mouse teratocarcinomas has made possible a number of new biochemical, immunological, and genetic approahes to the study of early mammalian development.
β‐Adrenoceptor stimulation potentiates insulin‐stimulated PKB phosphorylation in rat cardiomyocytes via cAMP and PKA (2010)

beta-adrenoceptors are strong regulators of PKB phosphorylation via cAMP and PKA when insulin is present. We hypothesize that PKB mediates important signalling in the heart during beta-adrenergic receptors stimulation.
West Virginia Corporate Law: Is It "Broke"? (1997)

We are all familiar with the cliche "if it ain't broke, don't fix it." The sentiment is as applicable to law as it is to the rest of life. When a law does what it is intended to do, legislators and courts should leave it alone. However, when a law no longer serves its intended purpose, it is "broke," and should be revised. The question is whether West Virginia's corporate law is "broke." In 1974, the West Virginia Legislature adopted the West Virginia Corporation Act (the "Act").' The Act brought then modem standards of corporate law to West Virginia. Since that adoption, corporate law in West Virginia has remained virtually unchanged However, the model codes on which the Act was based have been substantially revised to reflect changes in business practices.3 Consequently, the Act no longer reflects modem standards of corporate law. The failure to keep pace with the evolving standards of corporate law is particularly interesting in light of the fact that the legislature has continually adopted and revised other business entity laws in the state to keep pace with modem standards.4 A corporate law that reflects less than modem standards does not necessarily translate into a broken corporate law. Modem standards of corporate law are not necessarily appropriate for West Virginia. It does, however, suggest that the time has come to reevaluate the Act. This Article begins that process.
Identification of the Skin Basement-Membrane Autoantigen in Epidermolysis Bullosa Acquisita (1984)

Epidermolysis bullosa acquisita is an acquired chronic blistering disease of the skin, in which separation of the skin occurs in the basement-membrane zone between the epidermis and the dermis. There is evidence that blistering is initiated by an immune process. Using serum samples from nine patients as a source of antibodies, we have identified a major protein of the basement membrane of human skin that serves as the antigen (or target) for autoantibodies in this disorder. This previously unrecognized protein, which consists of two components of 290,000 and 145,000 daltons, is distinct from other known components of the basement membrane. These studies provide evidence that epidermolysis bullosa acquisita is a specific disease that is different from other primary bullous diseases, such as bullous pemphigoid and pemphigus vulgaris, and suggest that the basement-membrane component that has been identified may have a role in normal epidermal–dermal adherence. (N Engl J Med 1984; 310:1007–13.)
In vivo dissolution behavior of various RF magnetron sputtered Ca-P coatings (1998)

Radiofrequency magnetron sputter deposition was used to deposit Ca-P sputter coatings on titanium discs, and these coatings were implanted subcutaneously into the backs of rabbits. Half of the as-sputtered coatings were subjected to additional heat treatment for 2 h at 500 degrees C. X-ray diffraction (XRD) demonstrated that annealing at 500 degrees C changed the amorphous sputtered coating into an amorphous-crystalline apatite structure. Scanning electron microscopic (SEM) examination of the sputtered coatings showed excellent coverage of the substrate surface. Annealing of the 4-microm-thick coatings resulted in the appearance of small cracks. SEM demonstrated that until 4 weeks of implantation, all heat-treated coatings were present and all amorphous coatings were completely or mostly dissolved. Fourier transform infrared spectroscopy showed the formation of carbonate apatite (CO3-AP) on these specimens. Furthermore, XRD analysis showed that these CO3-AP precipitated coatings disappeared after 8 weeks of implantation. On the other hand, SEM inspection of these specimens revealed that the 4-microm heat-treated coating was still partially maintained and that small Ca-P crystals were present on the titanium substrate. On the basis of these results, we conclude that apparently 0.1 microm heat-treated Ca-P sputter coating is of sufficient thicknesses to stimulate carbonate apatite deposition under in vivo conditions.
Pharmacotherapy of Addictive Disorders (2009)

Substance use disorders are highly prevalent in the United States and cause considerable damage to our society. They are underrecognized and undertreated despite a vast body of literature demonstrating the efficacy of treatment using both psychosocial and psychopharmacological modalities. For the last decade, research and progress into the biological basis of the addictive process has led to a rapidly growing number of pharmacological agents used to interrupt the addictive process at its various stages such as the initiation of substance abuse, the transition from abuse to dependence, and the prevention of drug reinstatement or relapse. Food and Drug Administration-approved medications exist for nicotine, alcohol, and opioid use disorders, and progress is being made to develop agents for stimulant use disorders. Regarding nicotine use disorders, nicotine replacement therapies,bupropion and varenicline, have Food and Drug Administration approval, and future options exist with endocannabinoid antagonists and immune therapy. Aversive agents, opiate antagonists, and glutamate based interventions are currently approved to treat alcohol use disorders with future promise with GABAergic, serotonergic, and endocannabinoid system agents. Opiate addiction is treated by approved agonist and antagonist mu-opioid medications with the future potential for agents that can modulate the stress systems and the iboga alkaloids. Although no pharmacotherapies are currently approved for cocaine addiction, promising lines of research include agents that affect dopaminergic, GABAergic, serotonergic,and glutamatergic systems as well as the promise for immune therapies.
The level of mRNA encoding the amphotropic retrovirus receptor in mouse and human hematopoietic stem cells is low and correlates with the efficiency of retrovirus transduction. (1996)

The low level of amphotropic retrovirus-mediated gene transfer into human hematopoietic stem cells (HSC) has been a major impediment to gene therapy for hematopoietic diseases. In the present study, we have examined amphotropic retrovirus receptor (amphoR) and ecotropic retrovirus receptor mRNA expression in highly purified populations of mouse and human HSC. Murine HSC with low to undetectable levels of amphoR mRNA and relatively high levels of ecotropic retrovirus receptor mRNA were studied. When these HSC were analyzed simultaneously for ecotropic and amphotropic retrovirus transduction, ecotropic provirus sequences were detected in 10 of 13 long-term repopulated animals, while amphotropic proviral sequences were detected in only one recipient. A second distinct population of murine HSC were isolated that express 3-fold higher levels of amphoR mRNA. When these HSC were analyzed simultaneously for ecotropic and amphotropic retrovirus transduction, 11 of 11 repopulated mice contained ecotropic provirus and 6 of 11 contained amphotropic provirus sequences, a significant increase in the amphotropic retrovirus transduction (P = 0.018). These results indicate that, among the heterogeneous populations of HSC present in adult mouse bone marrow, the subpopulation with the highest level of amphoR mRNA is more efficiently transduced by amphotropic retrovirus. In a related study, we found low levels of human amphoR mRNA in purified populations of human HSC (CD34+ CD38-) and higher levels in committed progenitor cells (CD34+ CD38+). We conclude that the amphoR mRNA level in HSC correlates with amphotropic retrovirus transduction efficiency.
A 40-GHz Frequency Divider in 0.18-&lt;tex&gt;$muhboxm$&lt;/tex&gt;CMOS Technology (2004)

An analysis of regenerative dividers predicts the required phase shift or selectivity for proper operation. A divider topology is introduced that employs resonance techniques by means of on-chip spiral inductors to tune out the device capacitances. Configured as two cascaded /spl divide/2 stages, the circuit achieves a frequency range of 2.3 GHz at 40 GHz while consuming 31 mW from a 2.5-V supply.
Validity of the Hopkins approximation in simulations of hyper-NA (NA>1) line-space structures for an attenuated PSM mask (2006)

In our previous work we have shown that as the NA of a lithographic projection system increases some of the simulation assumptions that are traditionally made - such as the so-called Hopkins assumption, i.e. the assumption that diffraction at the mask is independent of the angle of incidence of the illuminating light waves - break down, at least in some cases. Reliable simulation results will then only be obtained if this Hopkins assumption is eliminated, i.e. when the diffraction effect is reevaluated for each incident direction. The differences in the results between two such simulations, one using the Hopkins approach, the other with this assumption removed, have been demonstrated to be very significant in some case, but today there is no clear understanding when the removal of the Hopkins assumption is essential: a systematic study is not available. As simulations without the Hopkins approximation are significantly more time consuming than simulations done under the Hopkins assumption, a better understanding of which model can or must be used under which circumstances, would be of significant practical importance. The aim of this paper is to provide such a more systematic study for the case of 6% attenuated PSM with line/space structures targeting at a 45 nm resist linewidth for a variety of pitches, for a NA = 1.2 water immersion system. Standard lithographic metrics such as process windows will be used to compare the two simulation approaches. All this work will be done taking the mask topography and optical material parameters into account. As the polarization state of the mask-illumination will also greatly affect the imaging quality at hyper-NA, we will compare the results for different polarization states and illumination modes.
Two-dimensional gas chromatography with electron capture detection for the sensitive determination of specific mycobacterial lipid constituents (1989)

A method was developed for determining two characteristic mycobacterial lipid constituents, tuberculostearic acid (as its pentafluorobenzyl ester) and 2-eicosanol (as its pentafluorobenzoyl ester), by using gas chromatography with electron capture detection. A microprocessor-controlled column-switching system (two-dimensional gas chromatography) facilitated sample preparation and increased specificity. The usefulness of the technique was illustrated by its ability to reveal picogram amounts of tuberculostearate in a suspension of Mycobacterium leprae isolated from a naturally infected armadillo. Two-dimensional gas chromatography with electron capture detection may in some instances provide a convenient alternative to gas chromatography-mass spectrometry for use in demonstrating the presence of mycobacteria in a complex environment.
The role of innate immune cells in ocular ageing and pathological neovascularisation (2012)

Age-related macular degeneration (AMD) is the major cause of vision loss in the developed world. AMD is a chronic progressive disorder of the outer retina leading to vision loss from atrophy (geographic atrophy) and/or the development of choroidal neovascularisation (CNV). Mounting evidence indicates the importance of innate immunity in its pathogenesis. This thesis describes a programme of work conducted with the aim of further understanding the role of innate immune cells in AMD.&#13;\nAnalysis of mouse cell suspensions by flow cytometry demonstrated significantly greater densities of innate immune cell populations in the RPE-choroid than in the neurosensory retina. Dendritic cells accumulated in both tissues with increasing age, and this process was accelerated in mice deficient in a chemokine upregulated in the aged choroid - CCL2. Innate immune cells were recruited to CNV lesions in mouse models of laser-induced and spontaneous CNV. Increasing age was found to correlate with the extent of laser CNV lesion size but not with recruitment of innate immune cells. Impaired recruitment of innate immune cells in CCL2-deficient mice was associated with a smaller laser-CNV lesion size, which nonetheless increased with age. Attenuation of CNV lesion size by targeting key angiogenic pathways using the small molecule pazopanib or by direct targeting of innate immune cells by induction of alternative activation using a CD200R agonist was associated with subtle increases in the recruitment of innate immune cell subpopulations to CNV lesions.&#13;\nFindings did not support the hypothesis that age-related vulnerability to laser CNV is a consequence of age-related changes in innate immune cell populations. However, the results indicated that CCL2 controls dendritic cell migration in the ageing retina and the recruitment of innate immune cells to CNV lesions. Further investigation of these pathways may lead to better treatments in the prevention and management of AMD.
Molecular Orientation at Surfaces of Solids. II The Work of Adhesion of the Saturated Fatty Acids for Water (1928)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular Orientation at Surfaces of Solids. II The Work of Adhesion of the Saturated Fatty Acids for WaterA. H. NietzA. H. NietzMore by A. H. NietzCite this: J. Phys. Chem. 1928, 32, 4, 620–630Publication Date (Print):April 1, 1928Publication History Published online1 May 2002Published inissue 1 April 1928https://doi.org/10.1021/j150286a013RIGHTS & PERMISSIONSArticle Views20Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (346 KB) Get e-Alerts
COMPARATIVE STUDIES OF FEMTOSECOND TO MICROSECOND LASER PULSES ON SELECTIVE PIGMENTED CELL INJURY IN SKIN (1991)

Threshold radiant exposures for grossly apparent immediate whitening and ultrastructural alterations of melanosomes in black guinea pig skin were determined for a series of red visible laser pulses ranging from 4 x 10(-4) to 6.5 x 10(-14) s. Threshold exposures for melanosomal injury were found to be independent of pulsewidth when the pulsewidths were below the estimated thermal relaxation time of melanosomes. Threshold radiant exposures for melanosomal injury were found to increase when the pulsewidths were approximately equal to or above the thermal relaxation time of melanosomes. At longer pulse durations, fracturing of melanosomes was not observed despite the longer exposures necessary for injury. Instead, perimelanosomal vacuoles were noted. These findings are consistent with the theory of selective photothermolysis and provide evidence for the thermal initiation of melanosomal disruption.
Study of the pressures applied by a Chêneau brace for correction of adolescent idiopathic scoliosis (2008)

We performed a study on 32 idiopathic scoliotic patients (30 females, 2 males) treated with a Chêneau brace. Eighteen patients had a single right thoracic curve and 14 had a single right thoracolumbar curve. We used the TekScan system (ClinSeat Type 5315 Sensor, TekScan, Boston MA, USA) to measure pressures at the skin-brace interface, assess the effect of strap tension and analyze the variation of these pressures with position and activity. The TekScan device enabled identification of the pressure areas corresponding to the brace's three loading points. The pressure under the main pad had a greater mean value than the pressure under the two counter-pads. Tightening the straps led to a significant increase in the pressures, whatever the position studied or the curve pattern. Compared with the standing (reference) position, we observed significantly higher pressures during maximal inspiration (p < 0.001) and lower pressures during maximal expiration, in the prone position and after having risen from a lying position, for both thoracic curves (p < 0.001) and thoracolumbar curves (p < 0.01). The pressures for thoracolumbar curves were lower than those for thoracic curves, whatever the position studied and both before and after strap adjustment. For lying positions, lying on the right side produced the greatest increase pressure. Even though the TekScan system does not provide direct information on the correction of spinal curvature, it appears to be a useful tool in the treatment of scoliotic patients. Strap adjustment clearly influences the applied pressures - particularly those on the rib cage. During activity, there is a natural tendency to decrease the pressure; this justifies efforts to maintain strap tensions in general and during day wear in particular.
Reduction Photolithography Using Microlens Arrays:  Applications in Gray Scale Photolithography (2002)

This paper describes the application of reduction photolithography, using arrays of microlenses and gray scale masks, to generate arrays of micropatterns having multilevel and curved features in photoresist. This technique can fabricate, in a single exposure, three-dimensional microstructures (e.g., nonspherical microlens arrays) over areas of approximately 2 x 2 cm2. The simple optical configuration consisted of transparency film (having centimeter-sized features) as gray scale photomasks, an overhead projector as the illumination source, and arrays of microlenses as the size-reducing elements. Arrays of 40- and 100-microm lenses achieved a lateral size reduction of approximately 10(3) and generated patterns of well-defined, multilevel structures; these structures may find use in applications such as diffractive optics.
IS YOUR FLOOR BOX REALLY NECESSARY? (1988)

The ‘biscuit tin’ floor box with its piano‐hinged lid has become the standard vehicle for connecting the cable which is fed below the raised floor to the desk equipment. Sometimes the unit has been specially developed — at what one can only presume to be considerable cost in the case of prestigious buildings like the Norman Foster Hong Kong &amp; Shanghai Bank headquarters. In most installations, they are more mundane derivatives of the floor box which was housed in the floor screed and provided on a design module of something between 1.5 and 2m. Despite the greater flexibility supposedly inherent in a raised floor solution, the reality is that the floor box in the raised floor copes no better with the transition between the ‘secondary’ and the ‘tertiary’ wiring than it did in the screeded floor.
A Camera-Projector System for Real-Time 3D Video (2006)

In this paper, we describe a real-time 3D video system that is based on active stereo. Combining a NTSC standard camera/ projector equipment and a suitable color code, the geometric and photometric information of a scene is robustly obtained in 30fps. Our main motivation to develop this system is to create a platform for investigating the issues that will be posed by the next generation of digital video and how it can shape up new media.