gebhart/patrepeval huggingface dataset:
Pharmacological Properties of Heterocyclic Amidine Derivatives. II. Pharmacological Studies of Phenylguanylpiperazine Derivatives (1968)

1-Phenyl-4-guanylpiperazine sulfate [PGP] and its derivatives were synthesized. The effects of these compounds on blood pressure, the nictitating membrane and the action of sympathomimetic amines such as tyramine and norepinephrine were examined in spinal cats. The effects of these compounds on MAO activity were also examined using the rat liver enzyme preparation in vitro. The results obtained were briefly summarized as follows : 1) PGP derivatives caused a rise in blood pressure and a contraction of the nictitating membrane in spinal cats. 2) PGP derivatives potentiated a pressor effect and a contraction of the nictitating membrane induced by tyramine and norepinephrine in spinal cats. 3) Reserpine caused a long-lasting hypertension and contraction of the nictitating membrane in spinal cats, when the animals were pretreated with PGP. 4) PGP derivatives showed a property to inhibit monoamine oxidase (MAO) activity. 5) PGP inhibited the restoration of the pressor effect of tyramine by a slow intravenous infusion of norepinephrine.
A functional magnetic resonance imaging study of the effect of sacral neuromodulation on brain responses in women with Fowler’s syndrome (2009)

These data show that brain responses to bladder filling are abnormal in FS. The explanation for this that best explains the evidence is that the primary abnormality is an overactive urethra that generates abnormally strong inhibitory afferent signals, so effectively blocking bladder afferent activity at the sacral level and deactivating the PAG and higher centres, with consequent loss of bladder sensation and ability to void. Apparently, a normal mechanism for suppression of incontinence involving the striated urethral sphincter becomes exaggerated in FS and prevents voiding. SNM seems to act at the sacral level, by blocking inhibition by urethral afferents.
Verotoxin glycolipid receptors determine the localization of microangiopathic process in rabbits given verotoxin-1. (1992)

Infection with verotoxin-producing Escherichia coli has been implicated in the cause of hemolytic-uremic syndrome. Cases of thrombotic thrombocytopenic purpura and verotoxin infections have been also described. In this study we sought to determine the following: (1) whether verotoxin induces microvascular lesions in the rabbit, (2) the organ distribution of such lesions, and (3) the distribution of verotoxin glycolipid receptors in the various organs. Rabbits challenged with verotoxin-1 purified from E. coli O157:H7 had anorexia, lethargia, and limb paralysis; renal function, however, was normal. Central nervous system lesions found included pericellular and perivascular edema, focal hemorrhage, vascular lesions, and severe alterations of Purkinje cells. Histologic changes were also seen in the colon, with mucosal and submucosal edema and hemorrhage, and in the lung, with interstitial fibrosis and focal lymphohistiocitic infiltration. No lesions were detected in kidney, heart, liver, and spleen. Screening of various tissues for the presence of the verotoxin receptors revealed galabiosyl ceramide in the central nervous system and globotriosyl ceramide in the gastrointestinal tract, lung, and spleen. No receptors for verotoxin were found in the heart, liver, and kidney. These results indicate that organ localization of the disease in rabbits is dependent on the distribution of verotoxin receptors.
A novel Silicon-On-Insulator (SOI) MOSFET for ultra low voltage operation (2002)

To extend the lower bound of power supply voltage, we propose a variable threshold voltage MOSFET (VTMOS) built on silicon-on-insulator (SOI). Threshold voltage of VTMOS drops as gate voltage is raised, resulting in a much higher current drive than regular MOSEET, at low Vdd. On the other hand, V/sub t/ is high at V/sub gs/=O, thus the leakage current is low. The SOI devices used in the study were built on SIMOX wafers. A four terminal layout was used to provide separate source, drain, gate, and body contacts.
S-R Theory and Dynamic Theory 1 (1971)

Abstract S-R theorists formulate psychological laws in terms of the relationship between external events and observed behavior. The state of the organism delimits the applicability of the law. Dynamic theorists formulate psychological laws in terms of the relationship between the initial state of the organism Oa and its subsequent state On. The significance of the stimulus is determined by .Oa and the principle of equifinality implies the equivalence of a wide range of behaviors by which On may be reached. It is maintained that regardless of the methodological difficulties inherent in a theory which is based on variables that cannot be observed directly, dynamic theory is testable in principle and in practice. It is suggested that recent neurophysiological theory is incompatible with an S-R approach.
Properties of Cage Rearrangements Observed near the Colloidal Glass Transition (2002)

We use confocal microscopy to study particle motion in colloidal systems. Near the glass transition, motion is inhibited, as particles spend time trapped in transient "cages" formed by neighboring particles. We measure the cage sizes and lifetimes, which, respectively, shrink and grow as the glass transition approaches. Cage rearrangements are more prevalent in regions with lower concentrations and higher disorder. Neighboring rearranging particles typically move in parallel directions, although a nontrivial fraction moves in antiparallel directions, usually from particle pairs with initial separations corresponding to local maxima and minima of the pair correlation function g(r), respectively.
Inactivation of the DNA repair gene O6-methylguanine-DNA methyltransferase by promoter hypermethylation is associated with G to A mutations in K-ras in colorectal tumorigenesis. (2000)

O6-methylguanine DNA methyltransferase (MGMT) is a DNA repair protein that removes mutagenic and cytotoxic adducts from the O6 position of guanine. O6-methylguanine mispairs with thymine during replication, and if the adduct is not removed, this results in conversion from a guanine-cytosine pair to an adenine-thymine pair. In vitro assays show that MGMT expression avoids G to A mutations and MGMT transgenic mice are protected against G to A transitions at ras genes. We have recently demonstrated that the MGMT gene is silenced by promoter methylation in many human tumors, including colorectal carcinomas. To study the relevance of defective MGMT function by aberrant methylation in relation to the presence of K-ras mutations, we studied 244 colorectal tumor samples for MGMT promoter hypermethylation and K-ras mutational status. Our results show a clear association between the inactivation of MGMT by promoter hypermethylation and the appearance of G to A mutations at K-ras: 71% (36 of 51) of the tumors displaying this particular type of mutation had abnormal MGMT methylation, whereas only 32% (12 of 37) of those with other K-ras mutations not involving G to A transitions and 35% (55 of 156) of the tumors without K-ras mutations demonstrated MGMT methylation (P = 0.002). In addition, MGMT loss associated with hypermethylation was observed in the small adenomas, including those that do not yet contain K-ras mutations. Hypermethylation of other genes such as p16INK4a and p14ARF was not associated with either MGMT hypermethylation or K-ras mutation. Our data suggest that epigenetic silencing of MGMT by promoter hypermethylation may lead to a particular genetic change in human cancer, specifically G to A transitions in the K-ras oncogene.
Antibiotic proteins of human neutrophils. (1990)

Despite strenuous opposition, Ilya Metchnikov convinced the scientific world 1 10 years ago that phagocytosis has a major role in immunity to infection (1).' Metchnikov conjectured that phagocytes destroy microbes with their enzymes, or cy- tases as he called them, but proof of this had to wait for new tools. The field moved when Cohn and Hirsch (1) revealed the role of degranulation in phagocytosis. Hirsch described pha- gocytin (1). Spitznagel and Chi (1) and Zeya and Spitznagel (1) showed that phagocytin is not one but an array ofantibacterial proteins, uniquely cationic, associated with cytoplasmic gran- ules, and coated on bacteria during phagocytosis. Klebanoff described the myeloperoxidase (MPO),2 H202, Cl-antimicro- bial system (1), and Gray described the oxidative defect in neutrophils and monocytes of children with X-linked chronic granulomatous disease (2). The duality of the killing mecha- nisms, oxygen independent and oxygen dependent, and the association of many of their components with cytoplasmic granules became accepted. I deal here with one aspect of research on oxygen-independent mechanisms, the antibiotic proteins of human neutrophils. These proteins comprise at least three structurally different families that share the capacity to kill bacteria independent of oxygen and evidently, contrary to Metchnikov's conjecture, to do so independent of enzymic action.
A novel spatial and temporal correlation integrated based motion-compensated interpolation for frame rate up-conversion (2008)

In this paper, a novel motion-compensated interpolation (MCI) method for frame rate up-conversion based on spatial and temporal correlation integrated is presented. Comparing to conventional MCI method, there are four main advantages of the proposed algorithm. First, the motion vectors (MVs) embedded in the bit-stream are examined and corrected by the coding-type information, the spatial correlation in the MV field and the temporal correlation in the pixel field together. Second, constant motion velocity model based MV estimation for the blocks in the interpolated frame is used to gain the initial value with consideration of the correlation of the related MV. Third, further refinement on the estimated MVs is performed based on the spatial correlation in the interpolated frame and temporal correlation in the before and after frames together. Fourth, adaptive weighted bi-directional interpolation is applied to obtain final recovered pixels using the refined MVs. Experimental results show that the proposed algorithm achieves a significant increase on PSNR and also a great improvement of visual performance over the conventional method.
Biophysical Characterization of the Signature Domains of Thrombospondin-4 and Thrombospondin-2 (2005)

The signature domain of thrombospondins consists of tandem epidermal growth factor-like modules, 13 calcium-binding repeats, and a lectin-like module. Although very similar, the signature domains of thrombospondin-1 and -2 differ in several potentially important ways from the domains of thrombospondin-3, -4, and -5. We have compared matching recombinant segments representing the signature domains of thrombospondin-2 and -4. In the presence of 2 mM CaCl2, the far UV circular dichroism spectra of thrombospondin-2 and -4 constructs contain a strong negative band at 202 nm, but only the thrombospondin-2 construct has a band at 216 nm. Chelation of calcium shifted the negative bands to lower magnitudes. Titrations of the spectra demonstrated lower cooperativity and affinity for binding of calcium to thrombospondin-4 compared with thrombospondin-2. Atomic absorption spectroscopy demonstrated that the thrombospondin-4 constructs bind seven less calcium than the thrombospondin-2 construct at 0.6 mM CaCl2. In 2 mM CaCl2, the near UV circular dichroism spectra of thrombospondin-2, but not thrombospondin-4, contain a positive band at 292 nm that disappears upon calcium chelation. Intrinsic fluorescence spectra for both proteins were also sensitive to calcium, but the changes were simpler and more marked for thrombospondin-2 than for thrombospondin-4. In differential scanning calorimetry, the thrombospondin-2 construct melted in two distinct transitions at 53.5 and 81.8 degrees C, whereas the first transition for thrombospondin-4 constructs was observed at 63.5 degrees C. Thus, the studies revealed significant differences between the signature domains of thrombospondin-2 and thrombospondin-4 in calcium binding, fine structure, and inter-modular interactions.
Radial structure of a low-frequency atmospheric-pressure glow discharge in helium (2002)

The spatial structure of a low-frequency atmospheric-pressure glow discharge was studied experimentally. The radial current distribution and discharge light emission were simultaneously measured at different phases during the ac voltage cycle. The glow discharge is formed by a radially propagating ionization wave. We also observed discharge regimes with several current pulses per half cycle corresponding to the successive, spatially separated breakdowns.
Nano-optomechanical Actuator and Pull-Back Instability (2013)

This paper studies the nonlinear behavior of a nano-optomechanical actuator, consisting of a free-standing arc in a ring resonator that is coupled to a bus waveguide through evanescent waves. The arc deflects when a control light of a fixed wavelength and optical power is pumped into the bus waveguide, while the amount of deflection is monitored by measuring the transmission spectrum of a broadband probe light. This nanoactuator achieves a maximal deflection of 43.1 nm, with a resolution of 0.28 nm. The optical force is a nonlinear function of the deflection of the arc, leading to pull-back instability when the control light is red-tuned. This instability is studied by a combination of experiment and modeling. Potential applications of the nanoactuator include bio-nanomotor, optical switches, and optomechanical memories.
Bacterial Diversity in Human Subgingival Plaque (2001)

The purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16S rRNA sequences for all cultivable and not-yet-cultivated species of human oral bacteria. Subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16S ribosomal DNA (rDNA) bacterial genes from DNA isolated from subgingival plaque samples were PCR amplified with all-bacterial or selective primers and cloned into Escherichia coli. The sequences of cloned 16S rDNA inserts were used to determine species identity or closest relatives by comparison with sequences of known species. A total of 2,522 clones were analyzed. Nearly complete sequences of approximately 1,500 bases were obtained for putative new species. About 60% of the clones fell into 132 known species, 70 of which were identified from multiple subjects. About 40% of the clones were novel phylotypes. Of the 215 novel phylotypes, 75 were identified from multiple subjects. Known putative periodontal pathogens such as Porphyromonas gingivalis, Bacteroides forsythus, and Treponema denticola were identified from multiple subjects, but typically as a minor component of the plaque as seen in cultivable studies. Several phylotypes fell into two recently described phyla previously associated with extreme natural environments, for which there are no cultivable species. A number of species or phylotypes were found only in subjects with disease, and a few were found only in healthy subjects. The organisms identified only from diseased sites deserve further study as potential pathogens. Based on the sequence data in this study, the predominant subgingival microbial community consisted of 347 species or phylotypes that fall into 9 bacterial phyla. Based on the 347 species seen in our sample of 2,522 clones, we estimate that there are 68 additional unseen species, for a total estimate of 415 species in the subgingival plaque. When organisms found on other oral surfaces such as the cheek, tongue, and teeth are added to this number, the best estimate of the total species diversity in the oral cavity is approximately 500 species, as previously proposed.
Update on myositis-specific and myositis-associated autoantibodies (2000)

Myositis-specific autoantibodies or myositis-associated autoantibodies can often be found in serum of patients with polymyositis and dermatomyositis. The presence of these autoantibodies can be significant in patient diagnosis and classification. Recent studies have provided new information about many of these specific autoantibodies. Among the more important developments were identification of a new antisynthetase, reacting with asparaginyl-tRNA synthetase; the detection of antibodies to the tRNA(his) in a over a third of anti-Jo-1 sera; and the description of distinctive features of the histopathology of patients with anti-Jo-1. New information about the cellular role of the antigens was discovered, including a role for Mi-2 antigen in chromosomally-mediated regulation of transcription as part of a nucleosome remodeling complex, and a potential role for PM-Scl antigen in ribosomal RNA processing as part of an exosome. The reason for the production of the autoantibodies, and the reason particular antigens are targeted, are key questions. Recent studies have suggested that antigen cleavage during apoptosis, particularly by granzyme B, may be an important factor. Whether the antibodies play a role in tissue injury remains unknown.
Coordination Assemblies from a Pd(II)-Cornered Square Complex (2005)

The [enPd(II)]2+ (en = ethylenediamine) unit has emerged as a versatile building block in molecular self-assembly. In particular, the 90° coordination angle of the metal has been judiciously used in the design of new discrete two- and three-dimensional structures. Our last 15 years of work with the Pd(II)-cornered unit is summarized in this Account, from the spontaneous formation of a Pd4 square metal complex to a family of architectures such as cages, bowls, boxes, tubes, catenanes, and spheres.
De novo assembly of short sequence reads (2010)

A new generation of sequencing technologies is revolutionizing molecular biology. Illumina's Solexa and Applied Biosystems' SOLiD generate gigabases of nucleotide sequence per week. However, a perceived limitation of these ultra-high-throughput technologies is their short read-lengths. De novo assembly of sequence reads generated by classical Sanger capillary sequencing is a mature field of research. Unfortunately, the existing sequence assembly programs were not effective for short sequence reads generated by Illumina and SOLiD platforms. Early studies suggested that, in principle, sequence reads as short as 20-30 nucleotides could be used to generate useful assemblies of both prokaryotic and eukaryotic genome sequences, albeit containing many gaps. The early feasibility studies and proofs of principle inspired several bioinformatics research groups to implement new algorithms as freely available software tools specifically aimed at assembling reads of 30-50 nucleotides in length. This has led to the generation of several draft genome sequences based exclusively on short sequence Illumina sequence reads, recently culminating in the assembly of the 2.25-Gb genome of the giant panda from Illumina sequence reads with an average length of just 52 nucleotides. As well as reviewing recent developments in the field, we discuss some practical aspects such as data filtering and submission of assembly data to public repositories.
Horizontal well completions in Alaska (1990)

Horizontal well completion technology is developing rapidly. During the past four years, open-hole horizontal completions have given way to fully cemented liners and to sophisticated slotted, screened or ported liners equipped with external packers for zonal isolation. Selective stimulation, including fracturing, has been performed successfully between formation packers and in fully cemented horizontal liners. Coiled tubing technology is being developed world-wide to provide the means to log, perforate, stimulate and service horizontal wells. This article discusses horizontal well completion strategy as a function of placement, mechanics and production philosophy. Completion techniques used by BP Exploration (BPX) in Alaska are discussed, and evolving technology is explored in an attempt to draw conclusions and direction from our work in Alaska and from the growing bank of worldwide experience with horizontal well completions.
Experimental Identification and Quantification of Glucose Metabolism in Seven Bacterial Species (2005)

The structurally conserved and ubiquitous pathways of central carbon metabolism provide building blocks and cofactors for the biosynthesis of cellular macromolecules. The relative uses of pathways and reactions, however, vary widely among species and depend upon conditions, and some are not used at all. Here we identify the network topology of glucose metabolism and its in vivo operation by quantification of intracellular carbon fluxes from 13C tracer experiments. Specifically, we investigated Agrobacterium tumefaciens, two pseudomonads, Sinorhizobium meliloti, Rhodobacter sphaeroides, Zymomonas mobilis, and Paracoccus versutus, which grow on glucose as the sole carbon source, represent fundamentally different metabolic lifestyles (aerobic, anaerobic, photoheterotrophic, and chemoheterotrophic), and are phylogenetically distinct (firmicutes, gamma-proteobacteria, and alpha-proteobacteria). Compared to those of the model bacteria Escherichia coli and Bacillus subtilis, metabolisms of the investigated species differed significantly in several respects: (i) the Entner-Doudoroff pathway was the almost exclusive catabolic route; (ii) the pentose phosphate pathway exhibited exclusively biosynthetic functions, in many cases also requiring flux through the nonoxidative branch; (iii) all aerobes exhibited fully respiratory metabolism without significant overflow metabolism; and (iv) all aerobes used the pyruvate bypass of the malate dehydrogenase reaction to a significant extent. Exclusively, Pseudomonas fluorescens converted most glucose extracellularly to gluconate and 2-ketogluconate. Overall, the results suggest that metabolic data from model species with extensive industrial and laboratory history are not representative of microbial metabolism, at least not quantitatively.