gebhart/patrepeval huggingface dataset:
A New Step in the Treatment of Sickle Cell Disease (2017)

ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTA New Step in the Treatment of Sickle Cell DiseasePublished as part of the Biochemistry series "Biochemistry to Bedside"Paul R. Ortiz de Montellano*View Author Information University of California, San Francisco, San Francisco, California 94158-2517, United States*Telephone: 415 476-2903. E-mail: [email protected]Cite this: Biochemistry 2018, 57, 5, 470–471Publication Date (Web):November 27, 2017Publication History Received14 August 2017Published online27 November 2017Published inissue 6 February 2018https://pubs.acs.org/doi/10.1021/acs.biochem.7b00785https://doi.org/10.1021/acs.biochem.7b00785editorialACS PublicationsCopyright © 2017 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views5787Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (302 KB) Get e-AlertscloseSUBJECTS:Adenine,Genetics,Monomers,Peptides and proteins,Pharmaceuticals Get e-Alerts
High Temperature Ultra Stable Close-Coupled Catalysts (1995)

&lt;div class="htmlview paragraph"&gt;Close-coupled catalysts are being actively pursued by automotive engineers in order to meet stringent LEV/ULEV emission standards. However, future applications of close coupled catalyst will be exposed to 50 to 100°C higher operating temperatures with elimination of fuel enrichment to cool the catalyst. A successful close coupled catalyst technology must then be resistant to even higher temperature deactivation and yet continue to function at low temperature during the vehicle cold start. A close coupled catalyst technology is formulated through advanced catalyst design to meet LEV and ULEV emission standards after high temperature aging at 1050°C. This paper will show the inherent stability of the close coupled catalyst for both light-off temperature and steady state performance for aging temperatures up to 1100°C. In vehicle evaluation, experimental results on the effect of close coupled catalyst volume, precious metal loading and aging temperature on HC emissions during cold start and FTP will be presented. Results show that this close coupled catalyst technology achieves LEV/ULEV HC emissions after high temperature aging.&lt;/div&gt;
The Natural History of Alzheimer's Disease (1994)

These data provide the descriptive basis for future studies of this cohort. They indicate that longitudinal follow-up of demented cases increases accuracy of diagnosis, and that detailed cognitive testing aids in early classification.
Complement activation in synovial fluid and tissue from patients with juvenile rheumatoid arthritis (1986)

Synovial fluid (SF) and synovial tissue from 10 patients with juvenile rheumatoid arthritis were examined. The SFs were heterogeneous with respect to the degree of complement activation. Quantification of C3dg and the terminal complement complex revealed a positive correlation between activation of the early and the late parts of the cascade in all patients. The amount of C-reactive protein and the number of white blood cells in the SF correlated significantly with the degree of complement activation. Weak deposits of C3, C3dg, or terminal complement complex were observed in a few vessels in the synovial tissue from 5 of the patients. There was no correlation between complement activity in SF and in the corresponding tissue. Furthermore, there was no correlation between clinical activity in the joints and the degree of complement activation. It is concluded that there is a discrepancy between synovial tissue and synovial fluid with respect to complement activation. C-reactive protein may, to some extent, be responsible for activation in SF, and the accumulation of white blood cells may be due to complement activation products.
Erosion from Staphylococcus aureus Biofilms Grown under Physiologically Relevant Fluid Shear Forces Yields Bacterial Cells with Reduced Avidity to Collagen (2007)

An estimated 65% of infective diseases are associated with the presence of bacterial biofilms. Biofilm-issued planktonic cells promote blood-borne, secondary sites of infection by the inoculation of the infected sites with bacteria from the intravascular space. To investigate the potential role of early detachment events in initiating secondary infections, we studied the phenotypic attributes of Staphylococcus aureus planktonic cells eroding from biofilms with respect to expression of the collagen adhesin, CNA. The collagen-binding abilities of S. aureus have been correlated to the development of osteomyelitis and septic arthritis. In this study, we focused on the impact of CNA expression on S. aureus adhesion to immobilized collagen in vitro under physiologically relevant shear forces. In contrast to the growth phase-dependent adhesion properties characteristic of S. aureus cells grown in suspension, eroding planktonic cells expressed invariant and lower effective adhesion rates regardless of the age of the biofilm from which they originated. These results correlated directly with the surface expression level of CNA. However, subsequent analysis revealed no qualitative differences between biofilms initiated with suspension cells and secondary biofilms initiated with biofilm-shed planktonic cells. Taken together, our findings suggest that, despite their low levels of CNA expression, S. aureus planktonic cells shed from biofilms retain the capacity for metastatic spread and the initiation of secondary infection. These findings demonstrate the need for a better understanding of the phenotypic properties of eroding planktonic cells, which could lead to new therapeutic strategies to target secondary infections.
RANTES expression in psoriatic skin, and regulation of RANTES and IL-8 production in cultured epidermal keratinocytes by active vitamin D3 (tacalcitol) (1998)

The chemokine RANTES is a chemoattractant for eosinophils, T lymphocytes of memory phenotype and monocytes, suggesting that it plays an important part in chronic inflammatory and allergic diseases. In various types of cells, RANTES production is markedly induced by tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma in combination. Psoriasis vulgaris is a chronic cutaneous inflammatory disease. Cytokines and chemokines produced by T cells and epidermal keratinocytes, such as interleukin (IL) 8, are involved in the pathogenesis of psoriasis. T-cell clones obtained from psoriatic skin have been shown to produce the Th1 cytokine IFN-gamma. In addition, abnormal expression of proinflammatory cytokines including TNF-alpha has been observed in psoriatic lesions. These reports led us to hypothesis that psoriatic skin could provide epidermal keratinocytes with TNF-alpha and IFN-gamma, so that keratinocytes could produce RANTES. In this study, we addressed the question as to whether RANTES was involved in psoriasis vulgaris. Immunohistochemistry of skin biopsies showed RANTES was present in the intercellular spaces between epidermal keratinocytes, in the fully developed lesions from the middle to the edge of psoriatic plaques, but not in the perilesional uninvolved and healthy control skin. Further, we confirmed the production of RANTES, together with IL-8, by cultured normal human epidermal keratinocytes, using an enzyme-linked immunosorbent assay. Stimulation with TNF-alpha and IFN-gamma in combination synergistically increased the RANTES production in this system. These results clearly demonstrate the expression of RANTES in psoriatic lesions and suggest the involvement of this chemokine in the outcome of cutaneous inflammatory diseases. Tacalcitol (1 alpha,24(R)-dihydroxyvitamin D3), an active vitamin D3 analogue, inhibited RANTES and IL-8 production in cultured normal epidermal keratinocytes. This result indicates that active vitamin D3 is effective in the regulation of chemokine production by epidermal keratinocytes, which may partly account for its action as an antipsoriatic drug.
Autonomous vehicle self-localization based on abstract map and multi-channel LiDAR in urban area (2018)

Accurate vehicle self-localization is significant for autonomous driving. The localization techniques based on Global Navigation Satellite System (GNSS) cannot achieve the required accuracy in urban canyons. On the other hand, simultaneous localization and mapping (SLAM) methods suffer from the error accumulation problem. State-of-the-art localization approaches adopt 3D Light Detection and Ranging (Lidar) to observe the surrounding environment and match the observation with a priori known 3D point cloud map for estimating the position of the vehicle within the map. However, storing the massive point cloud needs immense storage on the vehicle, or it should be stored on servers, which makes the simultaneous downloading of the map by multiple vehicles another challenge. In this study, rather than employing the point cloud directly as the prior map, we focus on the abstract map of buildings, which is easy to extract, and at the same time apparently observable by Lidar. More especially, we proposed vehicle localization methods based on two different abstract map formats representing urban areas. The first format is the multilayer 2D vector map of building footprints, which represents the building boundaries using vectors (lines). The second format is the planar surface map of buildings and ground. These map formats share the same idea that the uncertainty (deviation) of each vector or planar surface is calculated and included in the map. Later in the localization phase, the observed data from Lidar is matched with the abstract map to obtain the precise location of the vehicle. Experiments conducted in a dense urban area of Tokyo show that even though we significantly shrank the map size, we could preserve the mean error of the localization.
Target Fabrication for NIF and Inertial Fusion Energy (2013)

Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text A. Nikroo, "Target Fabrication for NIF and Inertial Fusion Energy," in CLEO: 2013, OSA Technical Digest (online) (Optica Publishing Group, 2013), paper ATh3O.2. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Enhancing Altitude Accuracy in Automotive Navigation using MEMS Barometric Sensor with GPS (2008)

Nemerix has developed a solution that effectively blends the altitude from a GPS receiver with the altitude derived from a MEMS pressure sensor. While solutions that integrate the pressure sensor with dead reckoning systems for vertical accuracy improvements in pedestrian navigation have been previously proposed, the use of a pressure sensor based altitude filter that specifically aims at improving altitude in an automotive navigation solution has not received much attention. The reason for this is not so much the lack of applications for enhanced altitude but the challenges imposed by the effects of environmental changes on the pressure and the difficulties in calibrating the sensor. Nemerix has developed calibration and compensation techniques that overcome these challenges. With the proposed solution, it becomes possible to identify if a vehicle is traveling on the upper level or one of the lower levels in a multi-level or stacked road and enhance turn by turn navigation. Typically, the vertical accuracy that is achieved with GPS is lower than the horizontal accuracy. This effect is further aggravated because the lower road is obstructed by the upper road, and exhibits very high multipath that further degrades the vertical accuracy. By using a low cost MEMS pressure sensor in conjunction with high accuracy navigation solution the vertical performance can be significantly improved to the level where a typical road-to-road vertical distance of 10 meters or more can be unambiguously resolved. This paper provides details of the proposed pressure sensor calibration and altitude filtering techniques and provides results and analyses that highlight the performance improvement.
The association between patellar alignment and patellofemoral joint osteoarthritis features an MRI study (2007)

The results of our study clearly indicated that patellar alignment is associated with manifestations of PF OA such as cartilage thickness loss and BML.
Marked stimulation of lymphocyte-mediated attack on tumor cells by target-directed liposomes containing immune RNA. (1978)

A marked stimulation of normal guinea pig lymphocytes was obtained by incubating them with liposomes that contained both antibody to lymphocytes to provide "homing" of the vesicles and immune RNA isolated from guinea pigs immunized with syngeneic line 10 hepatocarcinoma cells. Tumor cell cytotoxicity was monitored by a 51Cr release assay. This target cell delivery of immune RNA by liposomes produced a dose-dependent stimulation up to 12 times that achieved by in vitro methods with naked immune RNA.
The Cleaved Peptide of PAR1 Results in a Redistribution of the Platelet Surface GPIb-IX-V Complex to the Surface-Connected Canalicular System (2000)

The only known function of the 41 amino acid cleaved peptide (TR1-41) of the seven transmembrane domain thrombin receptor (PARI) is to activate platelets (as determined by aggregation, surface P-selectin, and fibrinogen binding to activated GPIIb-IIIa). We now demonstrate that TR1-41 results in a concentration-dependent decrease in the platelet surface expression of each component of the GPIb-IX-V complex, as determined by flow cytometry with a panel of monoclonal antibodies (including 6D1, directed against the von Willebrand factor binding site on GPIbalpha, and TM60, directed against the thrombin binding site on GPIbalpha). TR1-41 also decreased ristocetin-induced platelet agglutination. Immunoblotting after incubation of platelets with TR1-41 revealed neither a loss of platelet GPIb nor increase in supernatant GPIb fragments. As demonstrated by immunoelectron microscopy, TR1-41 resulted in a redistribution of GPIb, GPIX, and GPV from the platelet surface to the surface-connected canalicular system (SCCS). In summary, the cleaved peptide (TR1-41) of PAR1 results in a redistribution of the platelet surface GPIb-IX-V complex to the SCCS, thereby negatively regulating the GPIbalpha binding sites for von Willebrand factor and thrombin.
Characteristics of Inner Ring (3 or 5) Monodeiodination of 3,5-Diiodothyronine in Rat Liver: Evidence Suggesting Marked Similarities of Inner and Outer Ring Deiodinases for Iodothyronines* (1982)

To examine the characteristics of inner ring monodeiodination of an iodothyronine, 3,5-diiodothyronine (3.5-T2; ∼5 × 10-7 M) was incubated in 0.1 M Tris buffer (final pH, 7.3) with rat liver homogenate (∼0.13 geq) for 30–45 min at 37 C, and the 3-monoiodothyronine (3-T1) generated during incubation was quantified by a specific RIA of ethanol extracts of the incubation mixture. The monodeiodination was influenced importantly by substrate concentration and the temperature (optimal, 37 C) and pH (optimal, 8.3) of incubation, suggesting that it is enzymic in nature; the Michaelis constant (Km) approximated 0.5–1.25 μM, whereas the Vmax approximated 25 pg/mg protein · min. Among various rat tissues, liver and kidney (kidney > liver) were more potent in inner ring monodeiodination of 3,5- T2 than brain, spleen, intestines, or muscle. Among the various subcellular fractions of liver homogenate, microsomes were more potent in the conversion of 3,5-T2 to 3-T1 than mitochondria, cytosol, or the starting homogenate, but plasma membrane was the most active fraction. The conversion of 3,5-T2 to 3-T1 in liver homogenate was 1) higher during incubation in nitrogen than air; 2) stimulated by dithiothreitol and inhibited by diamide and iodoacetic acid; 3) inhibited in a dose-dependent manner by rT3, 3′,5′-T2, ipodate, 3′-T1, T4, propylthiouracil, 8-anilino-1-naphthalene sulfonic acid, and sodium salicylate, but little or not at all by T3 (4 μM), methimazole (30 mM), calcium chloride (1 mM), or ascorbic acid (50 mM); and 4) inhibited by fasting of the rat for 4 days. 3′,5′-T2 inhibited monodeiodination of 3.5-T2 to 3-T1 in a noncompetitive manner. The enzymic nature of the inner ring monodeiodinase for 3.5-T2 as well as its organ distribution, subcellular location, and responses to all of the various manipulations were identical to those known for the outer ring monodeiodinase (s) for 3′,5′-T2, rT3, or T4; the only difference was a higher optimal pH (∼8.3) for the inner ring than for the outer ring (∼7.5) deiodinase. The data suggest that inner and outer monodeiodinases are exceedingly similar, and the possibility cannot be excluded that there is only one monodeiodinase for action on the various iodothyronines.
Similar UV responses are seen in a skin organ culture as in human skin in vivo (2002)

Ultraviolet radiation (UVR) plays an important role in the development of non-melanoma skin cancer. Most tumors develop in chronically sun-exposed skin, most often in cosmetically sensitive locations, where in vivo experiments may be difficult to perform. In this study, we describe a skin organ culture model with preserved normal morphology and intact response to UVR. Skin explants from chronically sun-exposed and non-sun-exposed skin were irradiated with artificial UVA+UVB with and without topical sunscreen. UV-induced DNA damage, epidermal p53 response and repair kinetics were analyzed using immunohistochemistry. Four hours after UV-irradiation epidermal keratinocytes showed a strong immunoreactivity for thymine-dimers. Gradual repair during an incubation time resulted in few residual thymine-dimers after 48 h. Repair appeared to be more efficient in chronically sun-exposed skin compared with non-sun-exposed skin. There was also an accumulation of p53 protein in epidermal keratinocytes, peaking at 4-24 h after irradiation. Large interindividual differences with respect to formation and repair of thymine-dimers as well as induction and duration of the p53 response were observed. Skin explants treated with topical sunscreen prior to UV-irradiation showed a clear reduction of thymine-dimers and p53 expression. The epidermal UV-responses and repair kinetics in organ-cultured skin were similar to what was found in vivo. Our data suggest that organ-cultured skin provides a valuable tool for studies of UV-induced epidermal responses in chronically sun-exposed skin.
Binding of ATP to Brain Glutamate Decarboxylase as Studied by Affinity Chromatography (1984)

The interactions of two forms of porcine brain glutamate decarboxylase (beta-GAD and gamma-GAD) with the effector ATP were studied by affinity chromatography. A third form, alpha-GAD, was only slightly retarded by the affinity matrix and was eluted in the buffer wash. The interaction of GAD with the ATP affinity matrix was qualitatively similar to its interaction with free ATP as reported in previous kinetic studies. The rank order of adenine nucleotides as eluting agents and affinity ligands was ATP greater than ADP greater than AMP. GAD was also eluted by its cofactor, pyridoxal 5'-phosphate, and this was enhanced by 1 mM Pi. In contrast, a high concentration (140 mM) of Pi by itself was required to elute the enzyme. GAD remained active while bound to the affinity column and was eluted in the holoenzyme form by ATP, indicating that the affinity ligand did not bind in the active site and did not displace catalytically active cofactor from the enzyme.
Serum interleukin 5 concentrations in atopic and non-atopic patients with glucocorticoid-dependent chronic severe asthma. (1994)

Interleukin 5 is detectable in the serum of a proportion of both atopic and non-atopic patients with chronic severe asthma, and concentrations in these patients were higher than in normal controls. These observations are compatible with the hypothesis that IL-5 release occurs in these patients during a period of stable asthma despite systemic glucocorticoid therapy.
Neurotoxicity associated with adenine arabinoside monophosphate in the treatment of chronic hepatitis B virus infection (1984)

Thirty-one patients with chronic HBV infection were randomized to receive a short or long course of ARA-AMP. Fifteen completed a 4 week course of treatment and 16 completed longer courses varying from 7 to 12 weeks. Eighteen patients did not experience any neurological side effects. Six patients had myalgia. This was not associated with any neurological deficit. Seven patients developed a sensory neuropathy with distressing pain and dysesthesiae in the feet and abnormalities in nerve conduction. The development of neurotoxicity was related to a long duration of treatment and a correspondingly higher total dose but there was no association with the severity of liver disease.