gebhart/patrepeval huggingface dataset:
Expression of the MC1 receptor gene in normal and malignant human melanocytes. A semiquantitative RT-PCR study. (1999)

Alpha melanocyte-stimulating hormone (MSH) and related proopiomelanocortin-derived (POMC) peptides bind to the melanocortin 1 receptor (MC1-R) of mammalian melanocytes and stimulate proliferation and melanogenesis. POMC transcripts and alpha-MSH-like immunoreactivity have been found in melanoma cells and a possible autocrine loop involving MC1-R and POMC-derived products has been proposed. Therefore, the alpha-MSH/MC1-R system plays a major role in the biology of melanocytes, and provides targets for melanoma diagnosis and therapy. However, the relative levels of MC1-R expression in normal melanocytes (NM) and melanoma cells are unknown, and it is still debated whether or not all human melanomas express the MC1-R. We describe a semiquantitative RT-PCR assay for MC1-R expression, using a competition vector generated by deleting 164 bp of the native gene. The competitor was employed to analyse a panel of human melanoma cells, tumour samples, giant congenital nevus cells (CNM) and normal melanocytes (NM). All samples were positive for MC1-R expression, but expression of the receptor gene did not correlate with that of tyrosinase. Expression levels were about 10 and 20 times higher for surgical specimens and cultured melanoma cells, respectively, than for NM, but comparable for CNM and NM. Thus, high MC1-R expression is a frequent event in malignant melanocytes, and might lead to a higher activity of the alpha-MSH/MC1-R system in melanoma cells as compared to normal melanocytes, for equal local concentrations of the hormone or related melanocortins.
Efficacy of a Monoclonal Antibody Directed against Tumor Necrosis Factor in Protecting Neutropenic Rats from Lethal Infection with Pseudomonas aeruginosa (1990)

A monoclonal antibody directed against murine tumor necrosis factor-alpha (TNF) was studied in a neutropenic rat model to determine its efficacy in protecting animals from lethal infection with Pseudomonas aeruginosa. Anti-TNF monoclonal antibody at a dose of 20 mg/kg given intravenously at 0 and 120 h resulted in a 53% survival rate (8/15) compared with no survival in control animals (0/15) (P less than .005). The combination of anti-TNF monoclonal antibody and oral ciprofloxacin at a suboptimal dose of 2.5 mg/kg/day resulted in a 100% survival rate in neutropenic animals (16/16), while ciprofloxacin alone produced only a 67% survival rate (10/15) during the 7-day period of neutropenia (P less than .05). Thus anti-TNF monoclonal antibody alone or in addition to antimicrobial agents improved survival in neutropenic animals after infection with P. aeruginosa.
Synthesis and Cancer Chemopreventive Activity of Zapotin, a Natural Product from Casimiroa edulis (2006)

An efficient method has been developed to synthesize zapotin (5,6,2',6'-tetramethoxyflavone), a component of the edible fruit Casimiroa edulis, on a multigram scale. The synthesis utilizes a regioselective C-acylation of a dilithium dianion derived from a substituted o-hydroxyactophenone to afford a beta-diketone intermediate that can be cyclized to zapotin in good overall yield, thus avoiding the inefficient Baker-Venkataraman rearrangement pathway. Zapotin was found to induce both cell differentiation and apoptosis with cultured human promyelocytic leukemia cells (HL-60 cells). In addition, the compound inhibits 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ornithine decarboxylase (ODC) activity with human bladder carcinoma cells (T24 cells), and TPA-induced nuclear factor-kappa B (NF-kappaB) activity with human hepatocellular liver carcinoma cells (HepG2 cells). These data suggest that zapotin merits further investigation as a potential cancer chemopreventive agent.
Functional genomics (1999)

Complete genome sequences are providing a framework to allow the investigation of biological processes by the use of comprehensive approaches. Genome analysis also is having a dramatic impact on medicine through its identification of genes and mutations involved in disease and the elucidation of entire microbial gene sets. Studies of the sequences of model organisms, such as that of the nematode worm Caenorhabditis elegans, are providing extraordinary insights into development and differentiation that aid the study of these processes in humans. The field of functional genomics seeks to devise and apply technologies that take advantage of the growing body of sequence information to analyze the full complement of genes and proteins encoded by an organism.
Vasopressin in sepsis and septic shock. (2010)

Arginine vasopressin (AVP) and its synthetic, long-acting analog terlipressin (TP) are potent alternative vasoconstrictors in the treatment of septic patients with catecholamine-refractive vasodilatatory shock. The results from one large randomized clinical trial suggest that AVP plus norepinephrine (NE) infusion is as safe and effective as treatment with NE alone in patients with septic shock. Because the desired effects of vasopressin analogs are basically related to their vasopressinergic effects via the V1a receptor, more selective V1 agonists, such as TP, may be more potent in reversing sepsis-related arterial hypotension. In this regard, recent evidence from small-scale studies suggests that continuous low-dose infusion rather than intermittent bolus injection of TP is associated with fewer side effects, such as depression of cardiac output and rebound arterial hypotension. However, because clinical data on the administration of TP in patients with sepsis are limited, it should not currently be used beyond the scope of controlled trials. The optimal time point for the initiation of therapy with vasopressin analogs has yet to be determined. While AVP and TP are commonly used as last-resort therapies in severe septic shock, some evidence supports the initiation of treatment in a less severe state of the disease.
Differences in gene expression between B-cell chronic lymphocytic leukemia and normal B cells: a meta-analysis of three microarray studies (2004)

We introduce a statistical approach within a Bayesian framework to combine the microarray data on matched genes from three investigations of gene expression profiling of B-cell chronic lymphocytic leukemia (CLL) and normal B cells (NBC) using three different microarray platforms, oligonucleotide arrays, cDNA arrays printed on glass slides and cDNA arrays printed on nylon membranes. Using this approach, we identified a number of genes that were consistently differentially expressed between CLL and NBC samples.
Spectrally encoded miniature endoscopy (2001)

Summary form only given. Many diagnostic imaging applications, including fetoscopy, mammary ductoscopy, and angioscopy, require minimization of endoscope diameters. Currently, the low number of resolvable points that are provided by small diameter (<0.5 mm) endoscopes limits the clinical uses of these devices. Since optical fibers are of finite diameter, only a limited number of fibers can be incorporated into one imaging bundle. For example, for a 0.5 mm diameter fiber optic imaging bundle with individual fiber diameters of 5 /spl mu/m, the total number of resolvable points is 8000. We describe an imaging device that uses spectral encoding to obtain real-time images. Miniature endoscopes based on this technique have the potential to increase the resolution by a factor of more than 20 over fiber-optic imaging bundles of the same diameter.
Systemic multicompartmental effects of the gut microbiome on mouse metabolic phenotypes (2008)

To characterize the impact of gut microbiota on host metabolism, we investigated the multicompartmental metabolic profiles of a conventional mouse strain (C3H/HeJ) (n=5) and its germ-free (GF) equivalent (n=5). We confirm that the microbiome strongly impacts on the metabolism of bile acids through the enterohepatic cycle and gut metabolism (higher levels of phosphocholine and glycine in GF liver and marked higher levels of bile acids in three gut compartments). Furthermore we demonstrate that (1) well-defined metabolic differences exist in all examined compartments between the metabotypes of GF and conventional mice: bacterial co-metabolic products such as hippurate (urine) and 5-aminovalerate (colon epithelium) were found at reduced concentrations, whereas raffinose was only detected in GF colonic profiles. (2) The microbiome also influences kidney homeostasis with elevated levels of key cell volume regulators (betaine, choline, myo-inositol and so on) observed in GF kidneys. (3) Gut microbiota modulate metabotype expression at both local (gut) and global (biofluids, kidney, liver) system levels and hence influence the responses to a variety of dietary modulation and drug exposures relevant to personalized health-care investigations.
Role of Circulating Peptide YY in the Inhibition of Gastric Acid Secretion by Dietary Fat in Humans (2000)

Release of PYY into the circulation is not responsible for inhibition of gastrin-stimulated gastric acid secretion by dietary fat.
Computer-assisted preoperative planning, interactive surgery, and frameless stereotaxy. (1992)

Frame-based stereotactic systems provide valuable localization information for the performance of neurosurgical procedures. State-of-the-art systems provide for sophisticated preoperative planning and intraoperative interactive help with resection of predefined tissue volumes, providing assistance with resection of intrinsic brain tumors whose margins may not be readily visible at the time of surgery. There remain, however, a majority of neurosurgical procedures that would benefit from some form of localization but for which the application of a cumbersome frame and arc system is inconvenient. Frameless stereotactic localization or "cranial-based" localization provides a rapid and convenient means for computer-interactive localization and surgery for many of these cases. The stereotactic operating arm system is designed to complement frame-based stereotactic surgery, bringing standard neurosurgical procedures into the realm of computer-assisted interactive localization. The pace of progress is such that eventually, some form of computer interactive cranial localization will become a common neurosurgical tool. The concept of frameless localization is taken a step further in the frameless radiosurgical system described above. It is reasonable to think that this concept of "automated feature recognition," whether for recognizing scalp or bone contours, will ultimately become the foundation of procedures requiring cranial localization with reference to previously obtained digital images.
Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions (1985)

The broad-host-range vectors pSUP104, pSUP106, pSUP204, pSUP304, and pSUP404 are based on conventional Escherichia coli vectors (such as pBR325 and pACYC184) which have been modified to include the mobilization and broad-host-range replication functions of the IncQ plasmid RSF1010. These vector plasmids now can be maintained in a wide range of bacterial genera including Rhizobium, Agrobacterium, and Pseudomonas. They are efficiently mobilized by RP4 and thus are of particular interest for bacteria refractory to transformation. They offer the selection markers and cloning sites characteristic of the basic E. coli vectors. Therefore, they can be applied and adapted to a variety of cloning strategies. However, the cloning of very large fragments (e.g., in cosmid hybrids of pSUP106) was found to affect the stability of the recombinant molecules in a Rec+ background. This instability was not observed with smaller inserts of about 5 kilobases.
Effect of cardiac lymph flow obstruction on cardiac collagen synthesis and interstitial fibrosis (2006)

The effect of chronic cardiac lymphatic obstruction on the myocardial synthesis of collagen type I and III was investigated in a rabbit model. In the lymphatic obstruction group (n=16), plasma C-terminal propeptide type I procollagen (PICP) and N-terminal propeptide type III procollagen (PIIINP) were elevated at 7, 14 and 30 days after the operation (p<0.05). The elevated PICP and PIIINP returned to the pre-operation values 60 days after the operation. The myocardial expression of collagen type I and III mRNA were also enhanced in the lymphatic flow obstruction group. Plasma PICP, PIIINP and myocardial collagen type I and III mRNA remained unchanged in the control group (n=16). We concluded that chronic obstruction of cardiac lymph flow leads to enhanced myocardial collagen synthesis in rabbits. The enhanced collagen synthesis starts within seven days after lymphatic obstruction and subsides after 60 days.
Internal anterior chamber diameter using optical coherence tomography compared with white-to-white distances using automated measurements (2006)

Anterior chamber measurement using optical coherence tomography (Visante OCT) was easy to handle and showed good repeatability. The internal horizontal diameter of the AC was larger than the horizontal corneal diameter determined by automated WTW measurements (IOLMaster, Orbscan IIz). Optical coherence tomography with the Visante OCT allows direct measurement of the AC width.
Apolipoprotein A-I: structure–function relationships (2000)

The inverse relationship between high density lipoprotein (HDL) plasma levels and coronary heart disease has been attributed to the role that HDL and its major constituent, apolipoprotein A-I (apoA-I), play in reverse cholesterol transport (RCT). The efficiency of RCT depends on the specific ability of apoA-I to promote cellular cholesterol efflux, bind lipids, activate lecithin:cholesterol acyltransferase (LCAT), and form mature HDL that interact with specific receptors and lipid transfer proteins. From the intensive analysis of apoA-I secondary structure has emerged our current understanding of its different classes of amphipathic alpha-helices, which control lipid-binding specificity. The main challenge now is to define apoA-I tertiary structure in its lipid-free and lipid-bound forms. Two models are considered for discoidal lipoproteins formed by association of two apoA-I with phospholipids. In the first or picket fence model, each apoA-I wraps around the disc with antiparallel adjacent alpha-helices and with little intermolecular interactions. In the second or belt model, two antiparallel apoA-I are paired by their C-terminal alpha-helices, wrap around the lipoprotein, and are stabilized by multiple intermolecular interactions. While recent evidence supports the belt model, other models, including hybrid models, cannot be excluded. ApoA-I alpha-helices control lipid binding and association with varying levels of lipids. The N-terminal helix 44-65 and the C-terminal helix 210-241 are recognized as important for the initial association with lipids. In the central domain, helix 100-121 and, to a lesser extent, helix 122-143, are also very important for lipid binding and the formation of mature HDL, whereas helices between residues 144 and 186 contribute little. The LCAT activation domain has now been clearly assigned to helix 144-165 with secondary contribution by helix 166-186. The lower lipid binding affinity of the region 144-186 may be important to the activation mechanism allowing displacement of these apoA-I helices by LCAT and presentation of the lipid substrates. No specific sequence has been found that affects diffusional efflux to lipid-bound apoA-I. In contrast, the C-terminal helices, known to be important for lipid binding and maintenance of HDL in circulation, are also involved in the interaction of lipid-free apoA-I with macrophages and specific lipid efflux. While much progress has been made, other aspects of apoA-I structure-function relationships still need to be studied, particularly its lipoprotein topology and its interaction with other enzymes, lipid transfer proteins and receptors important for HDL metabolism.
Immunologic and pathologic manifestations of the infection of rhesus monkeys with simian immunodeficiency virus of macaques. (1990)

The striking similarities between simian immunodeficiency virus (SIV)-induced disease in macaque monkeys and HIV-induced disease in humans make the SIV-induced macaque monkey an extraordinarily important model for the study of AIDS. The most significant difference between these lentivirus-induced syndromes is the more rapid progression of disease in SIV-infected monkeys. The immunologic and pathologic manifestations of SIV infections in rhesus monkeys are described.
Strain Engineering for Fully-Depleted SOI Devices (2010)

Abstract not Available.
Characteristics of fritting contacts utilized for micromachined wafer probe cards (2000)

We have investigated the relationship between contact forces and the fritting which should be utilized for making contact to integrated circuit pads in micromachined wafer probe cards. Micromachined probe cards are requisite to tests of higher pad-density and smaller pad-pitch chips with high-speed signals above 1 GHz. In addition, if a microactuator is integrated into each probe, we can realize a novel probe card in which contacts can directly be switched on and off. The critical problem of the micromachined probe cards, however, is that each micromachined probe cannot generate or endure the force required to break the oxide on an Al pad surface mechanically. To overcome the problem, we planned to reduce the required contact force by putting the fritting effect to good use. For that, in this article, we have measured forces required for making contact to Al pads and contact resistance when utilizing the fritting process for the break of the pad surface oxide. Furthermore, we have also measured the force necessary to disconnect the contact made by the fritting, since it is important to design the probe dimensions and integrated actuators. As a result, we have found that the fritting makes it possible to get low resistance contact to Al pads without applying external forces. Moreover, the contact resistance and the adhesion force, when using electroplated Au bumps as the contact probe, were found to be one-third of those when using electroplated Ni bumps, respectively. This indicates that the Au bump is more suitable for the probe of micromachined probe cards.
In-situ sampling system for thermal volatilization analysis of nonvolatile materials by gas chromatography-mass spectrometry (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn-situ sampling system for thermal volatilization analysis of nonvolatile materials by gas chromatography-mass spectrometryJacob. ShenCite this: Anal. Chem. 1977, 49, 6, 886–888Publication Date (Print):May 1, 1977Publication History Published online1 May 2002Published inissue 1 May 1977https://pubs.acs.org/doi/10.1021/ac50014a057https://doi.org/10.1021/ac50014a057research-articleACS PublicationsRequest reuse permissionsArticle Views54Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Shear effects on thermoplastic foam nucleation (1993)

Abstract Polyethylene foam experiments were conducted on a twin screw foam extruder to study the process effects upon foam nucleation. The results indicate that, other than quantity of nucleators, throughput rate and die opening are important process parameters, underscoring the importance of shear in the die on nucleation. It is also found that shear effects increase as nucleator level increases. A modified cavity model shows at least qualitative agreement with experimental results. Dimensionless Capillary number, ratio of shear force to surface tension force, provides evidence that shear force rather than shear rate alone is the principal parameter affecting foam nucleation.
Recovery of lasing properties of dye solutions after their photolysis (1976)

An investigation was made of the mechanism of photolysis of ethanol solutions of rhodamine 6G. It was found that the ethanol photolysis products (acetic and formic acids) affected the rate of photoconversion of the rhodamine 6G molecules. A method was suggested for prolonging the service life of active media in the form of ethanol solutions of rhodamine 6G, cresyl violet, and 7-acetoxy-3-phenylcoumarin.
Beyond the Scalpel: Targeting Hedgehog in Skin Cancer Prevention (2010)

This perspective places the article by Tang et al. in this issue of the journal (beginning on page 25) in the context of recent work defining the hedgehog signaling pathway as a central etiologic factor and as a therapeutic target in basal cell cancer. Tang et al. show that inhibition of cyclooxygenase activity, either genetically (in a relevant mouse model) or pharmacologically (in the mouse and in patients highly predisposed to develop basal cell skin cancers), may suppress basal cell carcinogenesis. This new study of cyclooxygenase inhibition, together with recent data on the efficacy of hedgehog pathway inhibition, offers new hope for patients at a high risk for basal cell cancer.
Effect of the antiendotoxic agent, taurolidine, in the treatment of sepsis syndrome (1995)

Taurolidine had no beneficial therapeutic effect on the outcome of patients admitted to the intensive therapy unit with sepsis syndrome, using clinical, bacteriologic outcomes, progression of endotoxemia, resolution of organ failure, and 28-day mortality rate as end points.
High mobility two-dimensional electron gas in AlGaN/GaN heterostructures grown by plasma-assisted molecular beam epitaxy (1999)

High quality AlGaN/GaN heterostructures have been grown by radio-frequency plasma-assisted molecular beam epitaxy on n-type GaN templates grown on sapphire by metal organic chemical vapor deposition. The unintentionally doped Al0.12Ga0.88N/GaN heterostructure exhibits a 77 K Hall mobility of 14 500 cm2/Vs and a 12 K mobility of 20 000 cm2/Vs (ns=5.0×1012 cm−2). A room temperature mobility of 1860 cm2/Vs (ns=4.8×1012 cm−2) was calculated for the two-dimensional electron gas channel using a two layer model from the measured mobility for the whole structure (template plus heterostructure). Magnetoresistance measurements at 4.2 K showed well-resolved Shubnikov–de Haas oscillations, which began at 2.6 T.
A single mycobacterial protein (hsp 65) expressed by a transgenic antigen-presenting cell vaccinates mice against tuberculosis. (1994)

We used a retroviral shuttle vector [pZIPNeoSV(X)] to transfect a monocyte-like murine tumour cell line (J774.G8) with the Mycobacterium leprae gene encoding heat-shock protein (hsp) 65. The antigen was expressed and presented on the surface of the transfected cell in association with major histocompatibility complex (MHC) class I and class II for recognition by T cells from specifically sensitized mice. We show here that when these transfected cells were used as a vaccine and introduced parentally into syngeneic (BALB/c) mice they conferred a remarkably high degree of protective immunity against subsequent challenge with either M. bovis bacillus Calmette-Guérin (BCG) or M. tuberculosis H37Rv.