gebhart/patrepeval huggingface dataset:
On expert performance in 3D curve-drawing tasks (2009)

A study is described which examines the drawing accuracy of experts when drawing foreshortened projections of 3D curves in ecologically-valid conditions. The main result of this study is that the distribution of error in expert drawings exhibits a bias similar to that previously observed in non-expert subjects, which is dependent on the degree of foreshortening of the imagined drawing surface. A review of existing perceptual studies also finds that only absolute 2D image-space error has been considered, which has been found to be largest with viewing angles of 25--55°. Our visualizations of 3D error indicate that 3D bias continues to increase with decreasing viewing angle. Based on these findings, we analyze current 3D curve drawing techniques for susceptibility to foreshortening bias, and make some suggestions for future sketch-based modeling systems.
A bi-planar coil system for nulling background magnetic fields in scalp mounted magnetoencephalography (2018)

Small, commercially-available Optically Pumped Magnetometers (OPMs) can be used to construct a wearable Magnetoencephalography (MEG) system that allows large head movements to be made during recording. The small dynamic range of these sensors however means that movement in the residual static magnetic field found inside typical Magnetically Shielded Rooms (MSRs) can saturate the sensor outputs, rendering the data unusable. This problem can be ameliorated by using a set of electromagnetic coils to attenuate the spatially-varying remnant field. Here, an array of bi-planar coils, which produce an open and accessible scanning environment, was designed and constructed. The coils were designed using a harmonic minimisation method previously used for gradient coil design in Magnetic Resonance Imaging (MRI). Six coils were constructed to null B<sub>x</sub>, B<sub>y</sub> and B<sub>z</sub> as well as the three dominant field gradients dB<sub>x</sub>/dz, dB<sub>y</sub>/dz and dB<sub>z</sub>/dz. The coils produce homogeneous (within ±5%) fields or field gradients over a volume of 40 × 40 × 40 cm<sup>3</sup>. This volume is sufficient to contain an array of OPMs, mounted in a 3D-printed scanner-cast, during basic and natural movements. Automated control of the coils using reference sensor measurements allows reduction of the largest component of the static field (B<sub>x</sub>) from 21.8 ± 0.2 nT to 0.47 ± 0.08 nT. The largest gradient (dB<sub>x</sub>/dz) was reduced from 7.4 nT/m to 0.55 nT/m. High precision optical tracking allowed experiments involving controlled and measured head movements, which revealed that a rotation of the scanner-cast by ±34° and translation of ±9.7 cm of the OPMs in this field generated only a 1 nT magnetic field variation across the OPM array, when field nulling was applied. This variation could be further reduced to 0.04 nT by linear regression of field variations that were correlated with the measured motion parameters. To demonstrate the effectiveness of the bi-planar coil field cancellation system in a real MEG experiment, a novel measurement of retinotopy was investigated, where the stimulus remains fixed and head movements made by the subject shift the visual presentation to the lower left or right quadrants of the field of view. Left and right visual field stimulation produced the expected responses in the opposing hemisphere. This simple demonstration shows that the bi-planar coil system allows accurate OPM-MEG recordings to be made on an unrestrained subject.
Comparative Dynamics of Retrograde Actin Flow and Focal Adhesions: Formation of Nascent Adhesions Triggers Transition from Fast to Slow Flow (2008)

Dynamic actin network at the leading edge of the cell is linked to the extracellular matrix through focal adhesions (FAs), and at the same time it undergoes retrograde flow with different dynamics in two distinct zones: the lamellipodium (peripheral zone of fast flow), and the lamellum (zone of slow flow located between the lamellipodium and the cell body). Cell migration involves expansion of both the lamellipodium and the lamellum, as well as formation of new FAs, but it is largely unknown how the position of the boundary between the two flow zones is defined, and how FAs and actin flow mutually influence each other. We investigated dynamic relationship between focal adhesions and the boundary between the two flow zones in spreading cells. Nascent FAs first appeared in the lamellipodium. Within seconds after the formation of new FAs, the rate of actin flow decreased locally, and the lamellipodium/lamellum boundary advanced towards the new FAs. Blocking fast actin flow with cytochalasin D resulted in rapid dissolution of nascent FAs. In the absence of FAs (spreading on poly-L-lysine-coated surfaces) retrograde flow was uniform and the velocity transition was not observed. We conclude that formation of FAs depends on actin dynamics, and in its turn, affects the dynamics of actin flow by triggering transition from fast to slow flow. Extension of the cell edge thus proceeds through a cycle of lamellipodium protrusion, formation of new FAs, advance of the lamellum, and protrusion of the lamellipodium from the new base.
Analysis of Enzyme Structure and Activity by Protein Engineering (1984)

Abstract Structure‐activity relationships of enzymes can now be analyzed for the first time by the systematic alteration of protein structure. Recent developments in the chemical synthesis of DNA fragments and recombinant DNA technology enable the facile modification of proteins by highly specific mutagenesis of their genes. Kinetic analysis of the mutant enzymes combined with high‐resolution structural data from protein X‐ray crystallography allow direct measurements on the relationships between structure and function. In particular, the strength and nature of enzyme‐substrate interactions and their detailed roles in catalysis and specificity can now be studied. We have developed such analysis of enzyme structure‐function by site‐directed mutagenesis of the tyrosyl‐tRNA synthetase from Bacillus stearothermophilus , concentrating so far on the subtle role of hydrogen bonding in both substrate specificity and catalysis. We find that the energetics of tyrosine and ATP binding must be analyzed in terms of an exchange reaction with solvent water. Based on this idea and structural data, we have engineered an enzyme of improved enzyme‐substrate affinity, and there thus appear to be real prospects of engineering proteins of new specificities, activities, and structural properties. We are also using protein engineering to gather direct information on the nature of enzyme catalysis. For example, we find the catalysis of formation of Tyr‐AMP from Tyr and ATP is due largely to electrostatic and hydrogen bonding interactions that are stronger in the transition state than in the ground state—a “strain” mechanism rather than acid‐base or covalent catalysis.
Digital processing of TV signals: system overview of a new IC solution (1988)

A TV receiver that incorporates new types of integrated circuits in order to provide improved picture quality and novel features is described. The substitution of digital signal processing technology wherever possible allows highly automated production and testing that results in cost-effective production. A very important system feature is the selected clock frequency of 13.5 MHz, which is a multiple of the line frequencies used for PAL, SECAM and NTSC. The feature box, a set of three integrated circuits designed to improve video performance, is described. The 100 Hz videotext function and the digital deflection controller are examined in detail.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Landelijke Bestuurskunde Dag '98 (1998)

Donderdag 16 april wordt voor de twaalfde keer de Landelijke Bestuurskunde Dag georganiseerd. Op dit moment houden zeven studenten van de faculteit Bestuurskunde van de Universiteit Twente zich bezig met het organiseren van deze dag.
On T2‐shortening by strongly magnetized spheres: A partial refocusing model (2002)

Computer simulations of water transverse relaxation induced by superparamagnetic particles are shown to disagree with the available theories, covering the slow diffusion domain. Understanding these new simulations, not in the slow diffusion domain, thus requires a new theoretical approach. A "partial refocusing model" is introduced for this purpose; it is based on a spatial division between an inner region where the gradients are too strong for the refocusing pulses to be efficient and an outer region where they are efficient. This model agrees with published simulations of relaxation induced by magnetic dipoles approximated as points. The validity domains of the various models are also compared.
Quantitative Analysis of Human Cruciate Ligament Insertions (1999)

The objective of this study was to provide quantitative data on the insertion sites of the cruciate ligaments. In the first part of the study, we determined the shapes and sizes of the insertions of the anterior and posterior cruciate ligaments (ACL and PCL), and further compared these data with the midsubstance cross-sectional areas of the ligaments. The cross-sectional area of the ACL and PCL midsubstance of 5 human knees was measured using a laser micrometer system. The insertion sites of each ligament were then digitized and the 2-dimensional insertion site areas were determined. Relative to the ligament midsubstance, the PCL tibial and femoral insertions were approximately 3 times larger, whereas those of the ACL were over 3.5 times larger. In the second part of the study, the ACLs and PCLs of 10 knees were each divided into their 2 components and the areas of each insertion were determined. Each component was approximately 50% of the total ligament insertion area and no significant difference between the 2 could be shown.
Supraspinal μ2-opioid receptors mediate spinal/supraspinal morphine synergy (1992)

TRIMU-5 (Tyr-D-Ala-Gly-NHC2H4CH(CH3)2) is a potent mu 2-opioid agonist/mu 1-opioid antagonist. A supraspinal dose (0.5 micrograms i.c.v.) of TRIMU-5 which is not analgesic when given alone antagonizes the analgesia produced by intracerebroventricular (i.c.v.) morphine, a mu 1 action. In contrast, in a synergy model consisting of the simultaneous administration of intrathecal morphine (0.1 micrograms) with multiple doses of i.c.v. morphine, the same supraspinal TRIMU-5 dose (0.5 micrograms i.c.v.) enhances analgesia. Supraspinal TRIMU-5 also potentiates spinal morphine directly, shifting its dose-response to the left. These results imply that within the brainstem mu 1 receptors mediate supraspinal analgesia while mu 2 receptors mediate the synergy with spinal mu systems.
High-Resolution Liquid Chromatography of Fluorescent Dye-Labeled Nucleic Acids (1994)

Using 100 mM of triethylammonium acetate as ion-pairing reagent, phosphodiester oligonucleotides labeled fluorescently at their 5' terminus could be separated successfully on alkylated nonporous 2.3-microns poly(styrene-divinylbenzene) particles by means of high-resolution liquid chromatography. Applying excitation wavelengths of 490, 520, 550, and 575 nm, respectively, optimum sensitivity was achieved for the fluorophores 5-carboxyfluorescein, 2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein, N,N,N',N'-tetramethyl-6-carboxyrhodamine, and 6-carboxy-X-rhodamine (FAM, JOE, TAMRA, and ROX, respectively) at emission wavelengths of 520, 550, 580, and 605 nm, respectively. With calibration curves being linear over at least three orders of magnitude, the lower detection limits were 0.5, 2, 2, and 3 fmol, respectively. Depending on the type of fluorescent dye attached, retention times increased in the order JOE < FAM < TAMRA < ROX. Subsequently, fluorescent oligonucleotides were employed to prime polymerase chain reactions (PCR). Again the fluorophores were found to increase the retention times of double-stranded nucleic acids, but to a lesser degree than those of single-stranded oligonucleotides. Using a single FAM label attached to one of the two PCR primers, the sensitivity of fluorescence detection was found to be approximately 1 fmol or 30-70 times higher than that of uv absorbance detection depending on the length of the PCR product. Since the technique allows the separation of PCR products differing only 4 to 8 base pairs in length within a size range of 50 to 200 base pairs, it may be employed for the quantitative assessment of competitive PCR.
Angiogenesis: a curse or cure? (2005)

Angiogenesis, the growth of new blood vessels is essential during fetal development, female reproductive cycle, and tissue repair. In contrast, uncontrolled angiogenesis promotes the neoplastic disease and retinopathies, while inadequate angiogenesis can lead to coronary artery disease. A balance between pro-angiogenic and antiangiogenic growth factors and cytokines tightly controls angiogenesis. Considerable progress has been made in identifying these molecular components to develop angiogenesis based treatments. One of the most specific and critical regulators of angiogenesis is vascular endothelial growth factor (VEGF), which regulates endothelial proliferation, permeability, and survival. Several VEGF based treatments including anti-VEGF and anti-VEGF receptor antibodies/agents are in clinical trials along with several other antiangiogenic treatments. While bevacizumab (anti-VEGF antibody) has been approved for clinical use in colorectal cancer, the side effects of antiangiogenic treatment still remain a challenge. The pros and cons of angiogenesis based treatment are discussed.
Procedure for diethoxymethylation of ketones (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProcedure for diethoxymethylation of ketonesWilliam L. Mock and Hwei-Ru TsouCite this: J. Org. Chem. 1981, 46, 12, 2557–2561Publication Date (Print):June 1, 1981Publication History Published online1 May 2002Published inissue 1 June 1981https://pubs.acs.org/doi/10.1021/jo00325a023https://doi.org/10.1021/jo00325a023research-articleACS PublicationsRequest reuse permissionsArticle Views831Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Heparanase: A Rainbow Pharmacological Target Associated to Multiple Pathologies Including Rare Diseases (2016)

In recent years, heparanase has attracted considerable attention as a promising target for innovative pharmacological applications. Heparanase is a multifaceted protein endowed with enzymatic activity, as an endo-β-D-glucuronidase, and nonenzymatic functions. It is responsible for the cleavage of heparan sulfate side chains of proteoglycans, resulting in structural alterations of the extracellular matrix. Heparanase appears to be involved in major human diseases, from the most studied tumors to chronic inflammation, diabetic nephropathy, bone osteolysis, thrombosis and atherosclerosis, in addition to more recent investigation in various rare diseases. The present review provides an overview on heparanase, its biological role, inhibitors and possible clinical applications, covering the latest findings in these areas.
p53 α-Helix mimetics antagonize p53/MDM2 interaction and activate p53 (2005)

Overexpression or hyperactivation of MDM2 contributes to functional inactivation of wild-type p53 in nearly 50% of tumors. Inhibition of p53 by MDM2 depends on binding between an NH(2)-terminal (residues 16-28) p53 alpha-helical peptide and a hydrophobic pocket on MDM2, presenting an attractive target for development of inhibitors against tumors expressing wild-type p53. Here we report that novel p53 alpha-helical peptide mimics based on a terphenyl scaffold can inhibit MDM2-p53 binding in vitro and activate p53 in vivo. Several active compounds have been identified that inhibit MDM2-p53 binding in an ELISA assay with IC(50) of 10 to 20 micromol/L and induce p53 accumulation and activation in cell culture at 15 to 40 micromol/L. These results suggest that p53 alpha-helical mimetics based on the terphenyl scaffold may be developed into potent p53 activators.
A 9.8 GHz back-gate tuned VCO in 0.35 μm CMOS (2003)

A fully-integrated voltage controlled oscillator (VCO) has been one of the focal points of CMOS RF design activities in recent years. As potential applications extend to higher frequencies, CMOS VCO designs have pushed from ultra-high frequency (UHF) into super-high frequency (SHF). The requirement for fully-integrated VCOs to operate with low voltage, low power and low phase noise usually leads to the use of relatively large transistors in a process where the quality factor (Q) of the resonant tank is low. As the operating frequency further increases, the designs become more difficult due to the large parasitic capacitance and poor high-frequency performance of the transistors. This circuit demonstrates a technique which makes a transistor parasitic capacitance tunable through the use of its back-gate thereby reducing or eliminating the need for a varactor. In other words, the functionalities of transconductance (gm) and variable capacitance (/spl Delta/C) are realized in the MOS transistors at the same time by utilizing (where it is possible) all the four terminals of the transistors.
Decay‐accelerating factor (CD55) protects human immunodeficiency virus type 1 from inactivation by human complement (1995)

HIV-1, in contrast to animal retroviruses, is not lysed by human complement, but is readily inactivated by the sera from different animal species. To identify a possible species-specific protection mechanism. HIV-1 was expressed in cells of non-human origin. Recombinant HIV-1 virions that could encode the chloramphenicol acetyltransferase (CAT) protein were produced in African green monkey COS-1 cells, mink cells and, as a control, in human HEp-2 cells and were then used to infect CD4-positive target cells. Analysis of the CAT activity of the target cells revealed that fresh HIV-1-negative human serum reduced the infectivity of HIV-1 derived from monkey and mink cells five- to tenfold, but had no effect on HIV-1 produced in human cells. In addition, human serum efficiently lysed HIV-1 produced in non-human cells in contrast to HIV-1 originating from human cells, suggesting lysis as an important mechanism of virus inactivation. Mammalian cells are protected against lysis by homologous complement by membrane-bound regulatory molecules. Two of these complement inhibitors, namely decay-accelerating factor (DAF) and, to a lesser extent, CD59 were found on the surface of HIV-1 virions by means of a virus capture assay. Antibodies against DAF, but not against other host cell molecules found on the viral surface, efficiently blocked the resistance of HIV-1 produced in human cells to human complement. These results suggest that the acquisition of DAF during the budding process from human cells protects HIV-1 in a species-specific way against the attack of human complement.
Perception and Signal Transduction of Cytokinins (2003)

Cytokinins are plant hormones implicated in diverse and essential processes in plant growth and development, and key genes for the metabolism and actions of cytokinins have recently been identified. Cytokinins are perceived by three histidine kinases--CRE1/WOL/AHK4, AHK2, and AHK3--which initiate intracellular phosphotransfer. The final destination of the transferred phosphoryl groups is response regulators. The type-B Arabidopsis response regulators (ARRs) are DNA-binding transcriptional activators that are required for cytokinin responses. On the other hand, the type-A ARRs act as repressors of cytokinin-activated transcription. How phosphorelay regulate response regulators and how response regulators control downstream events are open questions and discussed in this review.
Analysis of the immune response elicited by a Multiple Antigen Peptide (MAP) composed of two distinct protective antigens derived from the parasite Schistosoma mansoni (1997)

In this report we analyse the immune response elicited by a Multiple Antigen Peptide (MAP), containing three peptide sequences derived from two distinct vaccine candidates against schistosomiasis; the Schistosoma mansoni 28 kDa Gluthatione-S-Transferase (Sm28GST) and the Schistosoma mansoni Triose-Phosphate-Isomerase (sTPI). We examined the immunogenicity of this construct, named MAP 'DA', in three distinct mouse strains. The B-cell response, studied by measuring the production of different IgG isotypes, was mainly directed against the peptide derived from the Sm28GST, but also against the whole Sm28GST protein. In contrast, the T-cell response, as assessed by proliferation assay and cytokine mRNA expression, was directed against the MAP construct, the peptides derived from the sTPI protein and the whole sTPI protein. Significantly, T-cells from all MAP 'DA'-immunized mice, restimulated in vitro was the sTPI antigen, expressed IFN-gamma specific messengers. This cytokine has been described to play a major role in the reduction of the Schistosoma mansoni pathology. We thus demonstrate that a single MAP construct, composed of peptides from distinct antigens of Schistosoma mansoni, induced a B- and T-cell response, including production of potentially protective IFN-gamma, irrespective of the MHC background.
Novel Small Molecule Inhibitor of C1s Exerts Cardioprotective Effects in Ischemia-Reperfusion Injury in Rabbits (2001)

Myocardial ischemia-reperfusion injury can be related to complement activation with generation of chemotactic agents, adhesion molecule expression, release of cytokines and oxygen-derived free radicals, and subsequent neutrophil accumulation. In the present study the cardioprotective effects of a novel highly selective small molecule C1s inhibitor (C1s-INH-248, Knoll) were examined in a rabbit model of myocardial ischemia (I) and reperfusion (R; i.e., 60 min I + 180 min R). In in vitro tests (enzyme activity and SRBC lysis) C1s-INH-248 demonstrated profound inhibitory potency. In vivo C1s-INH-248 (1 mg/kg body weight) administered 5 min before reperfusion significantly attenuated myocardial injury (31.9 +/- 2.5 vs 8.9 +/- 1.6% necrosis/area at risk; p < 0.01). The cardioprotective effect was dose dependent. The reduction of myocardial injury was also observed as diminished plasma creatine kinase activity in C1s-INH-248-treated animals (70.7 +/- 6.8 vs 45.1 +/- 3.9 U/g protein after 3 h of reperfusion, p < 0.05). Further, cardiac myeloperoxidase activity (i.e., a marker of PMN accumulation) in the ischemic and necrotic area was significantly reduced following C1s-INH-248 treatment (1.31 +/- 0.23 vs 0.4 +/- 0.05 U/100 mg tissue in necrotic area, p < 0.01). Thus, blocking the classical complement pathway with a highly specific and potent synthetic inhibitor of the activated C1 complex appears to be an effective mean to preserve ischemic myocardium from injury following reperfusion.