gebhart/patrepeval huggingface dataset:
[Sheep transgenic embryos produced by intracytoplasmic sperm injection]. (2002)

In this study, the possibility of sheep transgenesis by intracytoplasmic sperm injection (ICSI) was assessed. In experiment 1, activation of ovine oocytes matured in vitro in preparation for ICSI has been investigated with 3.42 mmol/L Ca2+ treatment, ionomycin alone and ionomycin followed by 6-dimethylaminopurine (DMAP) after 3-h delay (group 1, 2 and 3, respectively). After activation, the oocytes were then cultured in SOFaaBSA medium. Cleavage rates were significantly (P<0.05) different among three groups (18.4%, 91.8% and 71.7%, respectively). In additional culture, no parthenotes in group 1, whereas 11% and 17.4% in group 2 and 3 developed to the blastocyst stage. Therefore we used the third activation method in the following ICSI tests. In experiment 2, development of ovine oocytes after ICSI was investigated. Thawed semen from two rams was separated by Percoll centrifugation and was used for ICSI or in vitro fertilization (IVF) trails. A total of 71.8% of oocytes reached the 2-cell stage following living sperm injection, which was significantly (p>0.05) different from those following IVF (41.4%) and sham-ICSI (30.2%). After seven days' culture, no sham-injected oocytes developed into the blastocyst stage, although 7% in ICSI and 16.1% in IVF-oocytes developed into the blastocyst stage, but there was no significant difference in ICSI and IVF groups (p>0.05). In the further study, the possibility of sheep transgenesis by ICSI was assessed. After coinjection of ovine oocytes matured in vitro with dead sperm cold to -20 degrees C and exogenous DNA encoding green fluorescent protein (GFP), seventy-three percent of coinjected oocytes developed to 2-cell stage (33/45) and two of them were transgene-expressing embryos. Among ten embryos at the 16-cell stage, all embryonic cells in one transgenic embryo still expressed GFP. Four coinjected blastocysts were thawed and transferred to the uterine of the two progesterone-synchronized recipient ewe. No pregnancies were detected on the 60th day. These results suggested sheep transgenic embryos could be produced by ICSI and further studies should be performed.
Hydrajet Fracturing: An Effective Method for Placing Many Fractures in Openhole Horizontal Wells (1998)

Abstract To date, the art of effective openhole horizontal well fracturing is not well defined. Difficulties in regional sealing hamper the fracturing task, and results are generally suspect. Without proper isolation methods, the use of openhole horizontal well fracturing is limited. During many fracturing processes, including fracture acidizing, fracture or acid placement often occurs where fluid first contacts the borehole, often at the heel of the well. A new method is now available that combines hydrajetting and fracturing techniques. By using this new method, operators can position a jetting tool at the exact point where the fracture is required without using sealing elements. Unlike other techniques, this new method allows operators to place multiple fractures in the same well; these fractures can be spaced evenly or unevenly as prescribed by the fracture design program. Largesized fractures can be placed with this method. Because the method is simple, operators can economically bypass damage by placing hundreds of small fractures in a long horizontal section. To enhance the process even more, operators can use acid and/or propped sand techniques to place a combination of the two fracture types in the well. This paper discusses the basic principles of horizontal hydrajet fracturing and how Bernoulli's theorem was used to design a hydraj et fracturing technique. Laboratory test results for the new technique are provided on Page 4.
Three Dimensional Structure Determination of Botulinum Neurotoxin (1999)

Abstract : The immediate goals of the contract on the structure and function relationship of botulinum neurotoxin are: 1) Determine the three-dimensional structure of botulinum neurotoxin at atomic resolution by x-ray crystallography. 2) Based on the structure of the neurotoxin, understand the toxins mechanism of action. We have accomplished the first goal of determining the three-dimensional structure of the 150 kD botulinum neurotoxin serotype A. The toxin is Y-shaped, with a very long alpha-helical translocation domain forming the backbone of the structure. The translocation domain is composed almost entirely of helices, 2 of which are 95 A in length and form a pseudo-coiled coil. The binding domain and catalytic domain are more globular in shape, located at two different ends of the translocation domain. The overall dimensions of the protein are 120 A x 80 A x 40 A. A complete description of the three- dimensional structure is described in the report (the manuscript will he published in the September issue of Nature Structural Biology). We have recently collected data on an inhibitor complex and a receptor - complex. Refinement and analysis of the toxin with bound molecules will be completed within the next 12 months.
Introduction to FireGrid (2007)

Chapter 1 in the book: The Dalmarnock Fire Tests: Experiments and Modelling, Edited by G. Rein, C. Abecassis Empis and R. Carvel, Published by the School of Engineering and Electronics, University of Edinburgh, 2007. ISBN 978-0-9557497-0-4
Comparison of Thermal Insulation Performance of Fibrous Materials for the Advanced Space Suit (2003)

The current multi-layer insulation used in the extravehicular mobility unit (EMU) will not be effective in the atmosphere of Mars due to the presence of interstitial gases. Alternative thermal insulation means have been subjected to preliminary evaluation by NASA to attempt to identify a material that will meet the target conductivity of 0.005 W/m-K. This study analyzes numerically the thermal conductivity performance for three of these candidate insulating fiber materials in terms of various denier (size), interstitial void fractions, interstitial void media, and orientations to the applied temperature gradient to evaluate their applicability for the new Mars suit insulation. The results demonstrate that the best conductive insulation is achieved for a high-void-fraction configuration with a grooved fiber cross section, aerogel void medium, and the fibers oriented normal to the heat flux vector. However, this configuration still exceeds the target thermal conductivity by a factor of 1.5.
Analysis of immunoglobulin E VH transcripts in a bronchial biopsy of an asthmatic patient confirms bias towards VH5, and indicates local clonal expansion, somatic mutation and isotype switch events (1999)

Immunoglobulin E (IgE)-dependent mechanisms play a pivotal role in mediating allergic disease. Previously, VH-Cepsilon transcripts from blood or spleen of atopic asthmatics have been analysed for VH gene usage and patterns of somatic mutation. An over-representation of the minor VH5 family has been observed, consistent with a superantigen drive. As local mucosal events in IgE production may be more significant in the disease process, we have analysed VH-Cepsilon transcripts from a bronchial biopsy of a patient with severe asthma. VH5 predominance was confirmed with 10 of 30 unique clones derived from this family. Repeated sequences, some with intraclonal variation, revealed clonal expansion and continuing mutational activity at the site. Unexpectedly, three unmutated VH-Cepsilon sequences were found, indicating that isotype switching to IgE can occur without mutation. Detection of a sister clone with extensive mutations was again consistent with local mutational activity. Evidence for local isotype switching was obtained by identification of clonally related immunoglobulin M (IgM), immunoglobulin G (IgG) and immunoglobulin E (IgE) sequences. However, in contrast to findings in blood, no IgG4 transcripts clonally related to IgE were detected, suggesting that the balance between synthesis of IgG4 and IgE may differ between systemic and local sites. These data confirm a VH5 bias in IgE, and support the concept that IgE-synthesizing B cells arise via local differentiation.
Evaluation of GA-based dynamic route guidance for car navigation using cellular automata (2003)

This paper describes a practical dynamic route selection method and a method for its evaluation. A classification is established for the information required by drivers in selecting routes. The advantage of the method is that the driver situation is expressed by environment information, destination information and vehicle information. Reports on the evaluation of navigation on real road maps are few. Experiments with the system in a dynamic environment built from a real road map show that the GA-based method is superior to the Dykstra algorithm for use in practical car navigation devices.
Pericytes regulate the blood–brain barrier (2010)

The blood-brain barrier (BBB) consists of specific physical barriers, enzymes and transporters, which together maintain the necessary extracellular environment of the central nervous system (CNS). The main physical barrier is found in the CNS endothelial cell, and depends on continuous complexes of tight junctions combined with reduced vesicular transport. Other possible constituents of the BBB include extracellular matrix, astrocytes and pericytes, but the relative contribution of these different components to the BBB remains largely unknown. Here we demonstrate a direct role of pericytes at the BBB in vivo. Using a set of adult viable pericyte-deficient mouse mutants we show that pericyte deficiency increases the permeability of the BBB to water and a range of low-molecular-mass and high-molecular-mass tracers. The increased permeability occurs by endothelial transcytosis, a process that is rapidly arrested by the drug imatinib. Furthermore, we show that pericytes function at the BBB in at least two ways: by regulating BBB-specific gene expression patterns in endothelial cells, and by inducing polarization of astrocyte end-feet surrounding CNS blood vessels. Our results indicate a novel and critical role for pericytes in the integration of endothelial and astrocyte functions at the neurovascular unit, and in the regulation of the BBB.
A Phase 3 Trial of Bevacizumab in Ovarian Cancer (2011)

Bevacizumab improved progression-free survival in women with ovarian cancer. The benefits with respect to both progression-free and overall survival were greater among those at high risk for disease progression. (Funded by Roche and others; ICON7 Controlled-Trials.com number, ISRCTN91273375.).
Targeting of the CD33-calicheamicin immunoconjugate Mylotarg (CMA-676) in acute myeloid leukemia: in vivo and in vitro saturation and internalization by leukemic and normal myeloid cells (2001)

Antibody-targeted chemotherapy is a promising therapy in patients with acute myeloid leukemia (AML). In a phase II study of Mylotarg (CMA-676, gemtuzumab ozogamicin), which consists of a CD33 antibody linked to calicheamicin, saturation and internalization by leukemic and normal myeloid cells were analyzed in 122 patients with relapsed AML. Peripheral blood samples were obtained just before and 3 and 6 hours after the start of the first and second Mylotarg treatment cycles. Within 3 to 6 hours after infusion, near complete saturation of CD33 antigenic sites by Mylotarg was reached for AML blasts, monocytes, and granulocytes, whereas Mylotarg did not bind to lymphocytes. Saturation levels prior to the start of the second Mylotarg treatment cycle were significantly increased compared with background levels before the start of the first cycle. This apparently was caused by remaining circulating Mylotarg from the first treatment cycle (approximately 2 weeks earlier). On binding of Mylotarg to the CD33 antigen, Mylotarg was rapidly internalized, as determined by the decrease in maximal surface membrane Mylotarg binding. Internalization of Mylotarg was also demonstrated in myeloid cells in vitro and was confirmed by confocal laser microscopy. In vitro studies using pulse labeling with Mylotarg showed a continuous renewed membrane expression of CD33 antigens, which can significantly increase the internalization process and thereby the intracellular accumulation of the drug. Finally, Mylotarg induced dose-dependent apoptosis in myeloid cells in vitro. These data indicate that Mylotarg is rapidly and specifically targeted to CD33(+) cells, followed by internalization and subsequent induction of cell death.
Direct aperture optimization: A turnkey solution for step‐and‐shoot IMRT (2002)

IMRT treatment plans for step-and-shoot delivery have traditionally been produced through the optimization of intensity distributions (or maps) for each beam angle. The optimization step is followed by the application of a leaf-sequencing algorithm that translates each intensity map into a set of deliverable aperture shapes. In this article, we introduce an automated planning system in which we bypass the traditional intensity optimization, and instead directly optimize the shapes and the weights of the apertures. We call this approach "direct aperture optimization." This technique allows the user to specify the maximum number of apertures per beam direction, and hence provides significant control over the complexity of the treatment delivery. This is possible because the machine dependent delivery constraints imposed by the MLC are enforced within the aperture optimization algorithm rather than in a separate leaf-sequencing step. The leaf settings and the aperture intensities are optimized simultaneously using a simulated annealing algorithm. We have tested direct aperture optimization on a variety of patient cases using the EGS4/BEAM Monte Carlo package for our dose calculation engine. The results demonstrate that direct aperture optimization can produce highly conformal step-and-shoot treatment plans using only three to five apertures per beam direction. As compared with traditional optimization strategies, our studies demonstrate that direct aperture optimization can result in a significant reduction in both the number of beam segments and the number of monitor units. Direct aperture optimization therefore produces highly efficient treatment deliveries that maintain the full dosimetric benefits of IMRT.
Pyrazoles—A novel class of blocking agents for isocyanates (1994)

Abstract Pyrazoles were found as a novel class of blocking groups for isocyanates. Adhesive mixtures of pyrazole blocked isocyanates and amine terminated prepolymers like Jeffamines® (Texaco Chem. Co.) combine excellent reactivities (gelation times within minutes at 100–120°C), good latencies (more than 170 days at 40°C), and good adhesion properties on many substrates. The reactivity of pyrazole blocked isocyanates toward nucleophiles increases with the number of electron donor substituents on the pyrazole nucleus and, thus, can be fine tuned by the appropriate substitution pattern. This behavior contrasts sharply with that of phenolic blocked isocyanates, where reactivities with the same nucleophiles decrease with more and stronger electron donor substituents on the phenol nucleus. Therefore, different deblocking reaction mechanisms were proposed for pyrazole vs. phenol blocked isocyanates. The excellent latency of pyrazole blocked isocyanate/Jeffamine® mixtures is due to the insolubility of the two components at ambient temperature and the slow endothermic dissolution process at higher temperature. The good adhesion of formulations with pyrazole blocked isocyanates as reactive components on most plastic and metal substrates is ascribed to the primer action of the released blocking group. © 1994 John Wiley &amp; Sons, Inc.
[Study on the anti-carcinogenic effects of three compounds in Kaempferia galanga L]. (2002)

In order to study the anti-carcinogeneic effect of three compounds isolated from Kaempferia galanga L, EBV assay, ear edema test, ornithine decarboxylase (ODC) activity and two-stage-carcinogenisis test are applied. The results show that both -cis and -trans ethyl-p-methoxycinnamate has inhibitory effect in vitro, and also has inhibitory effects in TPA tests or croton oil-induced ear edema, ODC activity in specimen of mouse epidermis and extent of papilloma, indicating a relatively strong anti-carcinogenic potential of ethyl-p-methoxycinnamate in Kaempferia galanga L.
Impingementsyndrom (1999)

Erste Beschreibungen einer Pathologie im Bereich der Schulter lassen sich auf Duplay 1872 zurückführen, der eine subakromiale Bursitis beschrieb, die in posttraumatischen Schultern zu finden war. Er betrieb erste anatomische Studien und prägte den Begriff der Periarthropathia humeroscapularis. Dieser deskriptive Begriff, der auf die eigentliche zugrundeliegende Pathologie einer bestimmten Schultersymptomatik keinen Hinweis geben konnte, wurde später durch den Begriff des Impingements aus dem Angelsächsischen ersetzt. Impingement läßt sich direkt übersetzen in: anschlagen, berühren oder reiben, so daß also ein morphologischer deskriptiver Begriff durch einen funktionellen Begriff ersetzt wurde.
Hepatocyte Growth Factor Activator Inhibitor Type 1 Is a Specific Cell Surface Binding Protein of Hepatocyte Growth Factor Activator (HGFA) and Regulates HGFA Activity in the Pericellular Microenvironment (2000)

Hepatocyte growth factor activator (HGFA) is responsible for proteolytic activation of the precursor form of hepatocyte growth factor in injured tissues. To date, two specific inhibitors of HGFA have been identified, namely HGFA inhibitor type 1 (HAI-1) and type 2 (HAI-2)/placental bikunin (PB). Both inhibitors are first synthesized as integral membrane proteins having two Kunitz domains and a transmembrane domain, and are subsequently released from cell surface by shedding. Here we show that an active form of HGFA is specifically complexed with membrane-form HAI-1, but not with HAI-2/PB, on the surface of epithelial cells expressing both inhibitors. This binding required the enzyme activity of HGFA. The selective binding of HGFA to the cell surface HAI-1 was further confirmed in an engineered system using Chinese hamster ovary cells, in which only the cells expressing HAI-1 retained exogenous HGFA. The binding of HGFA to HAI-1 was reversible, and no irreversible modifications affecting the enzyme activity occurred during the binding. Importantly, HAI-1 and the HGFA.HAI-1 complex were quickly released from the cell surface by treatment with phorbol 12-myristate 13-acetate or interleukin 1beta accompanying the generation of 58-kDa fragments of HAI-1, which are less potent against HGFA, as well as significant recovery of HGFA activity in the culture supernatant. This regulated shedding was completely inhibited by BB3103, a synthetic zinc-metalloproteinase inhibitor. We conclude that HAI-1 is not only an inhibitor but also a specific acceptor of active HGFA, acting as a reservoir of this enzyme on the cell surface. The latter property appears to ensure the concentrated pericellular HGFA activity in certain cellular conditions, such as tissue injury and inflammation, via the up-regulated shedding of HGFA.HAI-1 complex. These findings shed light on a novel function of the integral membrane Kunitz-type inhibitor in the regulation of pericellular proteinase activity.
Bioequivalence of different dose-strength tablets of selexipag, a selective prostacyclin receptor agonist, in a multiple-dose up-titration study (2015)

Bioequivalence was demonstrated between 8 x 200 μg and 1 x 1,600 μg selexipag at steady state.
<title>Novel approach to exit pupil expansion for wearable displays</title> (2002)

The numerical aperture of the light emanating from display pixels in a given display system determines exit pupil size. In retinal scanning displays, the exit pupil is defined by the scanner optics, creating a rastered, projected image at an intermediate plane, typically resulting in an exit pupil approximately the size of an eye's pupil. Accounting for positional freedom of the eye and relative display placement defines the required expansion of the limited input NA for producing the desired exit pupil size for the display system. Although there are many approaches to performing this task of NA conversion, or exit pupil expansion, many of them exhibit wavelength dependencies, causing the envelope of the intensity profile in the exit pupil plane to be non- overlapping or inconsistent versus pixel location, resulting in uniformity or color variations and often efficiency loss. This paper will look at a microlens array approach to exit pupil expansion for color display systems that will be shown as wavelength independent in terms of diffraction envelope.
The alternatively initiated c-Myc proteins differentially regulate transcription through a noncanonical DNA-binding site. (1994)

The myc proto-oncogene family has been implicated in multiple cellular processes, including proliferation, differentiation, and apoptosis. The Myc proteins, as heterodimers with Max protein, have been shown to function as activators of transcription through an E-box DNA-binding element, CACGTG. We have now found that the c-Myc proteins regulate transcription through another, noncanonical, DNA sequence. The non-AUG-initiated form of the c-Myc protein, c-Myc 1, strongly and specifically activates transcription of the C/EBP sequences within the EFII enhancer element of the Rous sarcoma virus long terminal repeat. In contrast, comparable amounts of the AUG-initiated form, c-Myc 2, fail to significantly affect enhancer activity. However, both c-Myc proteins trans-activate the CACGTG sequence comparably. In addition, Myc/Max heterodimers, but not Max homodimers, bind to the EFII enhancer sequence in vitro. Finally, c-Myc 1 overexpression, but not c-Myc 2 overexpression, significantly inhibits cell growth. These results reveal new transcriptional activities for the Myc proteins and demonstrate that the different forms of the Myc protein are functionally distinct. These results also suggest an interplay between two different growth regulatory transcription factor families.
The effects of various hormones and growth factors on the growth of human insulin-producing cell line in serum-free medium (1997)

An insulin-producing cell line, HE, was derived from immortalized human pancreatic β-cells transformed by SV40 DNA, To make serum free medium specific for the cells, it was necessary to select basal medium, hormones, growth factors and coating materials. First of all, human insulin-producing cell line was adapted serially to the culture medium containing minimum required (1%) fetal calf serum (HB-1) from 10% fetal calf serum, The cells had a tendency to attach to the culture dishes coated with collagen or gelatin in the serum-free medium and showed a stronger attachment to collagen than to gelatin, For the basal medium selection, medium containing an equal mixture of RPMI 1640, Dulbecco's modified Eagle's medium, and Ham's F-12 (RDF) was shown to be the most suitable for the cells among 7 differently mixed basal media, The cells exhibited highly positive response to 7 factors including insulin-transferrin-selenium (ITS)complex in the basal medium. In addition, other 16 factors also influenced the cell growth positively, In contrast, 4 factors including aldosterone inhibited the cell growth, Cell growth was enhanced remarkably when the cells were cultured in the serum-free medium supplemented with all the seven positive factors, compared with in RDF serum-free medium. ITS and linoleic acid-BSA were found to be essential for the cell growth. The growth was much higher in the medium containing 6 strong positive factors without either dexamethasone, glucagon, or thyrocalcitonin than in the medium containing all the 7 strong positive factors, It is suggested that the interaction between the individual positive factors must be considered in choosing the factors with which the serum-free medium is supplemented.