gebhart/patrepeval huggingface dataset:
A Luminescence-Based Mercury Biosensor (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Luminescence-Based Mercury BiosensorMarko. Virta, Jorma. Lampinen, and Matti. KarpCite this: Anal. Chem. 1995, 67, 3, 667–669Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://pubs.acs.org/doi/10.1021/ac00099a027https://doi.org/10.1021/ac00099a027research-articleACS PublicationsRequest reuse permissionsArticle Views889Altmetric-Citations146LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Boronate-Mediated Biologic Delivery (2012)

Inefficient cellular delivery limits the landscape of macromolecular drugs. Boronic acids readily form boronate esters with the 1,2- and 1,3-diols of saccharides, such as those that coat the surface of mammalian cells. Here pendant boronic acids are shown to enhance the cytosolic delivery of a protein toxin. Thus, boronates are a noncationic carrier that can deliver a polar macromolecule into mammalian cells.
A Comparison of Transurethral and Transrectal Microwave Hyperthermia in Poor Surgical Risk Benign Prostatic Hyperplasia Patients (1991)

From 1987 to 1989, 36 poor surgical risk patients with benign prostatic hyperplasia (BPH) were treated with transrectal or transurethral microwave hyperthermia. Most of the 36 patients treated (85%) had severe signs and symptoms of urinary outflow obstruction. Of the patients 22 (61%) underwent transrectal and 14 (39%) underwent transurethral hyperthermia. Followup ranged from 10 to 28 months (mean 19 months) for the transrectal hyperthermia group and 7 to 16 months (mean 10 months) for the transurethral hyperthermia group. The patients were given 6 hyperthermia sessions of 30 minutes each with the temperature controlled on the rectal or urethral surface at 45C. Hyperthermia was well tolerated with mild acute toxicity and no late complications were observed. In the important subjective and objective parameters, major improvement was noted more frequently in the 14 transurethral than in the 22 transrectal hyperthermia treated patients (p less than 0.05). The Food and Drug Administration severity score, prostate volume, post-voiding residual volume and urethral flow showed substantial improvement in 79, 86, 79 and 79%, respectively, of the 14 transurethral hyperthermia treated patients compared to 41, 45, 82 and 82%, respectively, for the 22 transrectal hyperthermia treated patients. A prospective randomized trial comparing transrectal and transurethral hyperthermia is required to define the role of each treatment mode in patients with BPH.
A visual interface for synchronous collaboration and negotiated transactions (1996)

This paper introduces a visual interface for computer-supported cooperative work (CSCW). The interface is an extension of the editor interface of ESCHER, a prototype database system based on the extended non-first-normal-form data model. In ESCHER, the nested table approach is the paradigm for presenting data, where presenting includes browsing, editing and querying the database. Interaction is achieved by fingers generalising the well-known cursor concept. When several users are involved, the concept permits synchronous collaboration with the nested table acting as "whiteboard". We discuss its use in applications which require negotiated transactions, i.e. where the isolation principle of ACID-transactions gives way to negotiations. We also give examples of how interactive query formulation in a QBE-like fashion can support the collaboration. The arguments in the paper are mainly supported with screenshots taken from two applications, one of them also with non-textual data types which are seamlessly integrated into the nested tabular display paradigm.
Pain management prior to nasogastric tube placement: atomized lidocaine. (2015)

Nasogastric tube (NGT) insertion is often painful for patients of all ages. Randomized clinical trials in adult patients support the use of some form of topical lidocaine in reducing pain associated with NGT insertion. A review of pediatric evidence also confirms that NGT insertion is painful and provides guidance in determining lidocaine concentrations, dosages, and administration methods. The Iowa Model of Evidence-Based-Practice to Promote Quality Care provided the framework for development of a weight-based standard of practice (SOP) for administration of atomized lidocaine prior to NGT insertion for all patients. To facilitate usage, the orders for NGT placement and atomized lidocaine administration were linked in the electronic health record (EHR). Atomized lidocaine was administered via a patient-specific intranasal mucosal delivery device. Evaluation measures included pre- and post-implementation questionnaires which measured discomfort with NGT insertion in pediatric patients (0-10 scale; pre-implementation mean = 7.4; post-implementation mean = 6.5), monitoring utilization of atomized lidocaine via automated dispensing cabinet reports, soliciting comments from families and users, and monitoring institutional patient safety (incident) and adverse drug reaction reports. No patient safety or adverse drug reactions related to atomized lidocaine were identified post-implementation. Patients of all ages have benefited from administration of weight-based intranasal atomized lidocaine to decrease pain caused by NGT insertion. Ongoing safety evaluation and research is warranted since this is the first known report in the literature describing implementation of a weight-based dosing SOP.
Identification of the SH3 Domain of GAP as an Essential Sequence for Ras-GAP-Mediated Signaling (1993)

Guanosine triphosphatase activating protein (GAP) is an essential component of Ras signaling pathways. GAP functions in different cell types as a deactivator and a transmitter of cellular Ras signals. A domain (amino acids 275 to 351) encompassing the Src homology region 3 (SH3) of GAP was found to be essential for GAP signaling. A monoclonal antibody was used to block germinal vesicle breakdown (GVBD) induced by the oncogenic protein Ha-ras Lys12 in Xenopus oocytes. The monoclonal antibody, which was found to recognize the peptide containing amino acids 275 to 351 within the amino-terminal domain of GAP, did not modify the stimulation of the Ha-Ras-GTPase by GAP. Injection of peptides corresponding to amino acids 275 to 351 and 317 to 326 blocked GVBD induced by insulin or by Ha-Ras Lys12 but not that induced by progesterone. These findings confirm that GAP is an effector for Ras in Xenopus oocytes and that the SH3 domain is essential for signal transduction.
Radio Resource Allocation for a High Capacity Vehicular Access Network (2011)

Vehicular networks will enable a high number of services in intelligent transportation systems, and are also expected to improve the availability of wireless services to vehicles. In this paper we address the problem of establishing a vehicular network with high capacity using 802.11 and/or DSRC, and in particular we focus on frequency allocation and power control. We advocate a regular frequency reuse pattern that guarantees SINR requirements of the air interface. Resulting co-channel interference is then reduced using an adaptive power control algorithm. Simulation results for a highway scenario reveal that high capacities are possible with moderate infrastructure access point density.
Regulation of melanogenesis – controversies and new concepts (2008)

Despite many efforts, regulation of skin and hair pigmentation is still not fully understood. This article focuses mainly on controversial aspects in pigment cell biology which have emerged over the last decade. The central role of tyrosinase as the key enzyme in initiation of melanogenesis has been closely associated with the 6BH4 dependent phenylalanine hydroxylase (PAH) and tyrosine hydroxylase isoform I (THI) providing evidence for an old concept of the three enzyme theory in the initiation of the pigmentation process. In this context, it is noteworthy that intracellular L-phenylalanine uptake and turnover to L-tyrosine via PAH is vital for substrate supply of THI and tyrosinase. While PAH acts in the cytosol of melanocytes, THI and tyrosinase are sitting side by side in the melanosomal membrane. THI at low pH provides L-3,4-hydroxyphenylalanine L-DOPA which in turn is required for activation of met-tyrosinase. After an intramelanosomal pH change, possibly by the p-protein, has taken place, tyrosinase is subject to control by 6/7BH4 and the proopiomelanocortin (POMC) peptides alpha-MSH melanocyte stimulating hormone and beta-MSH in a receptor independent manner. cAMP is required for the activation of microphthalmia-associated transcription factor to induce expression of tyrosinase, for transcription of THI and for activation of PAH. The redundancy of the cAMP signal is discussed. Finally, we propose a novel mechanism involving H2O2 in the regulation of tyrosinase via p53 through transcription of hepatocyte nuclear factor 1alpha which in turn can also affect the POMC response.
Premature Expression of the Winged Helix Transcription Factor HFH-11B in Regenerating Mouse Liver Accelerates Hepatocyte Entry into S Phase (1999)

Two-thirds partial hepatectomy (PH) induces differentiated cells in the liver remnant to proliferate and regenerate to its original size. The proliferation-specific HNF-3/fork head homolog-11B protein (HFH-11B; also known as Trident and Win) is a family member of the winged helix/fork head transcription factors and in regenerating liver its expression is reactivated prior to hepatocyte entry into DNA replication (S phase). To examine whether HFH-11B regulates hepatocyte proliferation during liver regeneration, we used the -3-kb transthyretin (TTR) promoter to create transgenic mice that displayed ectopic hepatocyte expression of HFH-11B. Liver regeneration studies with the TTR-HFH-11B mice demonstrate that its premature expression resulted in an 8-h acceleration in the onset of hepatocyte DNA replication and mitosis. This liver regeneration phenotype is associated with protracted expression of cyclin D1 and C/EBPbeta, which are involved in stimulating DNA replication and premature expression of M phase promoting cyclin B1 and cdc2. Consistent with the early hepatocyte entry into S phase, regenerating transgenic livers exhibited earlier expression of DNA repair genes (XRCC1, mHR21spA, and mHR23B). Furthermore, in nonregenerating transgenic livers, ectopic HFH-11B expression did not elicit abnormal hepatocyte proliferation, a finding consistent with the retention of the HFH-11B transgene protein in the cytoplasm. We found that nuclear translocation of the HFH-11B transgene protein requires mitogenic signalling induced by PH and that its premature availability in regenerating transgenic liver allowed nuclear translocation to occur 8 h earlier than in wild type.
Effects of lithium chloride and ethacrynic acid on experimental polycystic kidney disease. (1988)

Hydrocortisone acetate is known to produce polycystic kidney disease when administered to susceptible animals in the perinatal period. To assess whether sodium and water transport mechanisms are involved in cyst pathogenesis, we administered lithium, an inhibitor of such transport in the distal nephron, and ethacrynic acid, an inhibitor of sodium transport in the Loop of Henle, to hydrocortisone acetate treated newborn rats. Hydrocortisone acetate alone resulted in significant polycystic kidney disease with the development of uremia, hypokalemia, and shortened life span. Co-administration of lithium and hydrocortisone acetate increased the severity of cystic tubular change and further compromised renal function. Ethacrynic acid reduced the degree of cyst formation induced by the combination of lithium chloride and hydrocortisone acetate but otherwise had no effect. We conclude that fluid and electrolyte disturbance plays a significant role in cyst formation, but we are unable to ascribe that effect to a single ion. We also conclude that the antinatriuretic effect of glucocorticoids is not the principal factor in glucocorticoid induced polycystic kidney disease.
Tumor-infiltrating Lymphocytes and Prognosis of Hepatocellular Carcinoma (1992)

Tumor-infiltrating lymphocytes (TIL) were isolated from 17 human hepatocellular carcinomas (HCC). The proliferation of TIL cultured with recombinant interleukin-2 (rIL-2) was evaluated. We also examined prognosis in relation to TIL. Successful expansion of TIL was achieved in 16 of the 17 lesions. In 10 of the 16, TIL increased more than 100-fold. Cytotoxicity to the allogeneic HCC cell line, HC-4, was demonstrated in all 13 TIL cultures tested. Maximum cytotoxicity was noted two to four weeks after culture. No statistical difference was observed either with respect to prognosis based upon growth rate or the cytotoxicity demonstrated in vitro. The initial number of TIL per unit weight of tumor was, however, significantly greater in the group for which the prognosis was good (19.0 +/- 5.1 x 10(6) vs. 5.6 +/- 1.6 x 10(6), P < 0.05). It would appear that greater lymphocytic tumor infiltration is a prognostic marker.
Host-virus genome interactions: macro roles for microRNAs (2007)

MicroRNAs are recently discovered small endogenous non-coding RNAs. These small RNAs of approximately 22 nucleotide length are crucial post-transcriptional regulators of gene expression in a wide spectrum of normal and abnormal biological processes including antiviral defence, oncogenesis and development in higher eukaryotes. Of late, a number of viruses have also been shown to encode for microRNAs. The host- and virus-encoded microRNAs and their targets together thus form a novel regulatory layer of genetic interactions between the host and the virus. Recent reports have thrown light on this new regulatory layer. A clear understanding of the cross-talk between the host and virus would not only enable us to understand the molecular basis of viral pathogenesis, but also enable us to develop better therapeutic strategies. This review discusses the intricacies of host-virus cross-talk mediated by microRNAs. Recent trends in this field and the challenges that need to be addressed are also discussed.
Facilitating real-time graph mining (2012)

Real-time data processing is increasingly gaining momentum as the preferred method for analytical applications. Many of these applications are built on top of large graphs with hundreds of millions of vertices and edges. A fundamental requirement for real-time processing is the ability to do incremental processing. However, graph algorithms are inherently difficult to compute incrementally due to data dependencies. At the same time, devising incremental graph algorithms is a challenging programming task.
Degradative enzymes in osteoarthritis (1999)

A central feature of the osteoarthritic disease process involves erosive destruction of the articular cartilage extracellular matrix (ECM) on the surfaces of diarthrotic joints. The resultant loss of joint function makes studies on mechanisms underlying ECM degradation critical for treatment of the disease and prevention of disability. Candidate pathways to account for the loss of cartilage involve expression of a combination of proteases that degrade the major cartilage matrix macromolecules, aggrecan and type II collagen. The specific types of enzymatic activities associated with the progressive removal of ECM and severity of joint disease include the matrix metalloproteinases, collagenase, gelatinase and aggrecanase(s). The degradative enzymes originate in synovial cells, cartilage cells, the chondrocytes, distributed within the ECM and leukocytes that actively invade the joint space. Specific enzymes arising from each of these tissues exhibit selective ECM degrading properties; the different categories of these tissue-derived enzymes will be discussed in this chapter. A perspective on the efficacy of existing agents and the potential for development of novel therapeutic agents is also included. While the degradative enzymes serve as a focal point for therapeutic intervention, a fundamental understanding of the mechanisms underlying degradative enzyme expression in osteoarthritis remains an important goal for prevention of disease.
Cerebrovascular permeability and delivery of gentamicin to normal brain and experimental brain abscess in rats (1984)

Antibiotics vary widely in their ability to penetrate the blood-brain barrier. In studies of 70 rats, the permeability of the normal blood-brain barrier to gentamicin was shown to be poor. In experimental brain abscesses, during the cerebritic stage of development, the penetration of intravenous antibiotics was increased compared to normal brain but was very inconsistent. Antibiotic delivery to brain abscess was not significantly altered with the administration of high-dose steroids, but the macrophage and glial response was markedly decreased with high-dose steroid therapy. Reversible osmotic blood-brain barrier modification with mannitol increased the delivery of gentamicin both to brain abscess and to the surrounding brain. It also resulted in more consistent tissue drug levels. The clinical implications of these studies suggest that, because of the inconsistent delivery of gentamicin to brain abscess, the therapeutic efficacy of medical management alone may be quite variable. This mode of therapy could possibly increase the efficacy of medical management of brain abscesses, especially in patients with multiple or surgically inaccessible brain abscesses.
Ultrahigh-frequency modulation of InGaAsP lasers (1986)

The design criteria of high-frequency lasers and the modulation performance of 1.3 μm vapor phase regrown buried heterostructure (VPR-BH) lasers are reviewed. The effect of nonlinear gain on dynamic response is discussed.
Identification and treatment of vulnerable plaque. (2004)

It is now well recognized that the rupture of vulnerable plaque, which consists of an atheromatous plaque core covered by a thin fibrous cap with ongoing inflammation, is a major cause of thrombus formation leading to the development of acute coronary syndrome. Several diagnostic techniques, including vascular imaging and serologic markers, are clinically available or currently under investigation for the detection of vulnerable plaque. A combination of several diagnostic modalities might allow effective screening of individuals at high risk for future cardiovascular events. Plaque stabilization with pharmacologic interventions--statins, beta-blockers, and angiotensin-converting enzyme inhibitors--might effectively prevent the development of acute coronary syndromes caused by plaque disruption.
Fully Automatic Registration of 3D Point Clouds (2006)

We propose a novel technique for the registration of 3D point clouds which makes very few assumptions: we avoid any manual rough alignment or the use of landmarks, displacement can be arbitrarily large, and the two point sets can have very little overlap. Crude alignment is achieved by estimation of the 3D-rotation from two Extended Gaussian Images even when the data sets inducing them have partial overlap. The technique is based on the correlation of the two EGIs in the Fourier domain and makes use of the spherical and rotational harmonic transforms. For pairs with low overlap which fail a critical verification step, the rotational alignment can be obtained by the alignment of constellation images generated from the EGIs. Rotationally aligned sets are matched by correlation using the Fourier transform of volumetric functions. A fine alignment is acquired in the final step by running Iterative Closest Points with just few iterations.
Development of a dielectric barrier discharge ion source for ambient mass spectrometry (2007)

A new ion source based on dielectric barrier discharge was developed as an alternative ionization source for ambient mass spectrometry. The dielectric barrier discharge ionization source, termed as DBDI herein, was composed of a copper sheet electrode, a discharge electrode, and a piece of glass slide in between as dielectric barrier as well as sample plate. Stable low-temperature plasma was formed between the tip of the discharge electrode and the surface of glass slide when an alternating voltage was applied between the electrodes. Analytes deposited on the surface of the glass slide were desorbed and ionized by the plasma and the ions were introduced to the mass spectrometer for mass analysis. The capability of this new ambient ion source was demonstrated with the analysis of 20 amino acids, which were deposited on the glass slide separately. Protonated molecular ions of [M + H](+) were observed for all the amino acids except for L-arginine. This ion source was also used for a rapid discrimination of L-valine, L-proline, L-serine and L-alanine from their mixture. The limit of detection was 3.5 pmol for L-alanine using single-ion-monitoring (SIM). Relative standard deviation (RSD) was 5.78% for 17.5 nmol of L-alanine (n = 5). With the advantages of small size, simple configuration and ease operation at ambient conditions, the dielectric barrier discharge ion source would potentially be coupled to portable mass spectrometers.
Columbia University’s Baseline Detectors for 374 LSCOM Semantic Visual Concepts (2007)

emantic concept detection represents a key requirement in accessing large collections of digital mages/videos. Automatic detection of presence of a large number of semantic concepts, such as person,” or “waterfront,” or “explosion”, allows intuitive indexing and retrieval of visual content t the semantic level. Development of effective concept detectors and systematic evaluation ethods has become an active research topic in recent years. For example, a major video retrieval enchmarking event, NIST TRECVID[1], has contributed to this emerging area through (1) the rovision of large sets of common data and (2) the organization of common benchmark tasks to erform over this data.