gebhart/patrepeval huggingface dataset:
CCI-Based Web Security: A Design Using PGP (1995)

We describe several ways of using the general-purpose Common Client Interface (CCI) for enhancing the security of communications on the World Wide Web (WWW). In this approach, the Web browser communicates with an external CCI application that handles the processing of digitally-signed and/or encrypted data. Our particular design uses the popular Pretty Good Privacy (PGP) software for all cryptographic operations. We define a PGP-CCI protocol which can be used to protect any HTTP message exchange, and we also describe a simpler scheme for PGP-protected form submissions (HTTP POSTs) which may be implemented without modifying web servers. Additionally, we point out the advantage of using a PGP-CCI application to handle pre-encrypted/signed Web documents in a user-friendly manner.
Increased Expression of Thrombospondin-1 in Vessel Wall of Diabetic Zucker Rat (2003)

Increased expression of TSP-1 in blood vessels in diabetes may represent a new link between diabetes, atherogenesis, and accelerated restenosis. This increase in TSP-1 production may be a direct response of vascular cells to glucose.
Inhibition of skin development by overexpression of transforming growth factor beta 1 in the epidermis of transgenic mice. (1993)

To assess the effect of transforming growth factor beta 1 on the skin in vivo, we have targeted its expression to the epidermis of transgenic mice. To ensure that active TGF-beta 1 was expressed, we used a porcine TGF-beta 1 cDNA with mutations of Cys-223-->Ser and Cys-225-->Ser, which allow constitutive activation. Mice expressing the mutant transforming growth factor beta 1 transgene exhibited a marked phenotype at birth. The skin was very shiny and tautly stretched. These animals were rigid and appeared to be restricted in their ability to move and breathe; death occurred within 24 hr. Histologically, the most prominent features of the skin were a compact orthohyperkeratosis and a reduction in the number of hair follicles. Pulse-labeling studies with 5-bromodeoxyuridine demonstrated a marked reduction in the number of replicating cells in the epidermis and hair follicles. Thus, the macro- and microscopic appearance of these mice, as well as their neonatal lethality, most likely result from inhibition of normal skin development and suppression of epithelial cell proliferation by the overexpression of transforming growth factor beta 1.
Virus Infection Switches TLR-3-Positive Human Neurons To Become Strong Producers of Beta Interferon (2005)

To study the capacity of human neurons to mount innate immunity responses to viral infections, we infected cells of a human postmitotic neuron-derivative cell line, NT2-N, with rabies virus (RABV) and herpes simplex type 1 (HSV-1). Changes in neuronal gene expression were analyzed by use of Affymetrix microarrays. Applying a twofold cutoff, RABV increased the transcription of 228 genes, and HSV-1 increased the transcription of 263 genes. The most striking difference between the two infections concerns genes involved in immunity. These genes represent 24% of the RABV-upregulated genes and only 4.9% of the HSV-1-upregulated genes. Following RABV infection, the most upregulated genes belong to the immunity cluster and included almost exclusively genes for beta interferon (IFN-beta) primary and secondary responses as well as genes for chemokines (CCL-5, CXCL-10) and inflammatory cytokines (interleukin 6 [IL-6], tumor necrosis factor alpha, interleukin 1 alpha). In contrast, HSV-1 infection did not increase IFN-beta gene transcripts and triggered the production of only IL-6 and interferon regulatory factor 1 mRNAs. The microarray results were confirmed by real-time PCR, immunocytochemistry, and enzyme-linked immunosorbent assay. Human neurons were found to express Toll-like receptor 3. They produced IFN-beta after treatment with poly(I:C) but not with lipopolysaccharide. Thus, human neurons can mount an innate immunity response to double-stranded RNA. These observations firmly establish that human neurons, in absence of glia, have the intrinsic machinery to sense virus infection.
Data display techniques for transportation analysis and planning: an investigation of three computer‐produced graphics (1977)

Graphics are a powerful but often costly means of communication. Computer‐drawn graphics offer a new and relatively inexpensive way to assist the communication of complex technical information to both planners and non‐technical people whose full potential is now beginning to be realized. This paper discusses the utility of graphics and introduces three computer‐drawn graphic techniques which may be useful, both for analysis and presentation of results, in the transportation planning process. CENVUE(S) produces a three‐dimensional, perspective‐view map, on which virtually any type of transportation data or performance indicator can be displayed. VAP is designed to display origin‐destination travel patterns in any region. TDN transforms a physical‐distance network into a time‐distance network so that effects of different speeds in the transportation network can be readily seen. The cost‐effectiveness of each technique is briefly discussed and some recommendations for evaluating computer graphics techniques are provided to aid the user in further assessing their utility in the transportation planning process.
Burden of major musculoskeletal conditions. (2003)

Musculoskeletal conditions are a major burden on individuals, health systems, and social care systems, with indirect costs being predominant. This burden has been recognized by the United Nations and WHO, by endorsing the Bone and Joint Decade 2000-2010. This paper describes the burden of four major musculoskeletal conditions: osteoarthritis, rheumatoid arthritis, osteoporosis, and low back pain. Osteoarthritis, which is characterized by loss of joint cartilage that leads to pain and loss of function primarily in the knees and hips, affects 9.6% of men and 18% of women aged > 60 years. Increases in life expectancy and ageing populations are expected to make osteoarthritis the fourth leading cause of disability by the year 2020. Joint replacement surgery, where available, provides effective relief. Rheumatoid arthritis is an inflammatory condition that usually affects multiple joints. It affects 0.3-1.0% of the general population and is more prevalent among women and in developed countries. Persistent inflammation leads to joint destruction, but the disease can be controlled with drugs. The incidence may be on the decline, but the increase in the number of older people in some regions makes it difficult to estimate future prevalence. Osteoporosis, which is characterized by low bone mass and microarchitectural deterioration, is a major risk factor for fractures of the hip, vertebrae, and distal forearm. Hip fracture is the most detrimental fracture, being associated with 20% mortality and 50% permanent loss in function. Low back pain is the most prevalent of musculoskeletal conditions; it affects nearly everyone at some point in time and about 4-33% of the population at any given point. Cultural factors greatly influence the prevalence and prognosis of low back pain.
Subcellular localization of the segment polarity protein patched suggests an interaction with the wingless reception complex in Drosophila embryos (1994)

The product of the segment polarity gene patched is a transmembrane protein involved in the cell communication processes that establish polarity within the embryonic segments of Drosophila. Monoclonal antibodies have been raised against the patched protein, and by immunoelectron microscopy part of the patched staining is found associated with discrete regions of the lateral plasma membrane of the embryonic epidermal cells. Using a mutation affecting endocytosis (shibire) we find that patched is a membrane-bound protein, which is internalized by endocytosis, and that the preferential sites of accumulation resemble the described localization of the cell-cell adhesive junctions of the epidermal cells. patched partially co-localizes with the wingless protein in the wingless-expressing and nearby cells, in structures that seem to be endocytic vesicles. These data suggest the interaction of patched protein with elements of the reception complex of wingless, as a way to control the wingless expression.
Inhibition of DNA Replication and Induction of S Phase Cell Cycle Arrest by G-rich Oligonucleotides (2001)

The discovery of G-rich oligonucleotides (GROs) that have non-antisense antiproliferative activity against a number of cancer cell lines has been recently described. This biological activity of GROs was found to be associated with their ability to form stable G-quartet-containing structures and their binding to a specific cellular protein, most likely nucleolin (Bates, P. J., Kahlon, J. B., Thomas, S. D., Trent, J. O., and Miller, D. M. (1999) J. Biol. Chem. 274, 26369-26377). In this report, we further investigate the novel mechanism of GRO activity by examining their effects on cell cycle progression and on nucleic acid and protein biosynthesis. Cell cycle analysis of several tumor cell lines showed that cells accumulate in S phase in response to treatment with an active GRO. Analysis of 5-bromodeoxyuridine incorporation by these cells indicated the absence of de novo DNA synthesis, suggesting an arrest of the cell cycle predominantly in S phase. At the same time point, RNA and protein synthesis were found to be ongoing, indicating that arrest of DNA replication is a primary event in GRO-mediated inhibition of proliferation. This specific blockade of DNA replication eventually resulted in altered cell morphology and induction of apoptosis. To characterize further GRO-mediated inhibition of DNA replication, we used an in vitro assay based on replication of SV40 DNA. GROs were found to be capable of inhibiting DNA replication in the in vitro assay, and this activity was correlated to their antiproliferative effects. Furthermore, the effect of GROs on DNA replication in this assay was related to their inhibition of SV40 large T antigen helicase activity. The data presented suggest that the antiproliferative activity of GROs is a direct result of their inhibition of DNA replication, which may result from modulation of a replicative helicase activity.
A versatile HDR video production system (2011)

Although High Dynamic Range (HDR) imaging has been the subject of significant research over the past fifteen years, the goal of acquiring cinema-quality HDR images of fast-moving scenes using available components has not yet been achieved. In this work, we present an optical architecture for HDR imaging that allows simultaneous capture of high, medium, and low-exposure images on three sensors at high fidelity with efficient use of the available light. We also present an HDR merging algorithm to complement this architecture, which avoids undesired artifacts when there is a large exposure difference between the images. We implemented a prototype high-definition HDR-video system and we present still frames from the acquired HDR video, tonemapped with various techniques.
Enantiospecific synthesis with amino acids. Part 1. Tryptophan as a chiron for the synthesis of α-substituted tryptophan derivatives (1991)

N α -Methoxycarbonyl-(S)-tryptophan methyl ester is cyclised with 85% phosphoric acid to give (2S,3aR,8aS)-1,2-bis(methoxycarbonyl)-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole which on reaction with toluene-p-sulphonyl chloride gives (2S,3aR,8aS)-1,2-bis(methoxycarbonyl)-8-(p-tolylsulphonyl)-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole. This compound undergoes deprotonation with lithium diisopropylamide to the corresponding enolate which is quenched with a variety of alkylating agents resulting in alkylation, with retention of configuration, at C-2 of the pyrroloindole system. Subsequent treatment with trifluoroacetic acid brings about cycloreversion affording essentially optically pure α-alkylated tryptophan derivatives. The process is also applied, in the R-series.
Bis(diisopropylamino)carbene (1996)

The first acyclic diaminocarbene 1 can be isolated by deprotonation of the corresponding formamidinium ion 2. In solution both compounds show dynamic behavior (interchange of iPr groups) with activation barriers of 53 and 55 kJmol−1, respectively.
Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain. (1990)

Plastins are a family of at least three cytoplasmic protein isoforms that are expressed differentially between cells of the hematopoietic lineages and cells of solid tissues. Expression of the L-plastin isoform appears to be restricted to replicating blood cells, and the two T-plastin isoforms appear to be restricted to replicating cells of solid tissues. However, L-plastin is induced in many human solid tumor-derived cells. We used the anchored polymerase chain reaction technique to amplify and clone the missing 5' ends of plastin mRNAs. We found that both plastin isoforms contain a potential calcium binding site near the N terminus.
B cell–intrinsic signaling through IL-21 receptor and STAT3 is required for establishing long-lived antibody responses in humans (2010)

Engagement of cytokine receptors by specific ligands activate Janus kinase-signal transducer and activator of transcription (STAT) signaling pathways. The exact roles of STATs in human lymphocyte behavior remain incompletely defined. Interleukin (IL)-21 activates STAT1 and STAT3 and has emerged as a potent regulator of B cell differentiation. We have studied patients with inactivating mutations in STAT1 or STAT3 to dissect their contribution to B cell function in vivo and in response to IL-21 in vitro. STAT3 mutations dramatically reduced the number of functional, antigen (Ag)-specific memory B cells and abolished the ability of IL-21 to induce naive B cells to differentiate into plasma cells (PCs). This resulted from impaired activation of the molecular machinery required for PC generation. In contrast, STAT1 deficiency had no effect on memory B cell formation in vivo or IL-21-induced immunoglobulin secretion in vitro. Thus, STAT3 plays a critical role in generating effector B cells from naive precursors in humans. STAT3-activating cytokines such as IL-21 thus underpin Ag-specific humoral immune responses and provide a mechanism for the functional antibody deficit in STAT3-deficient patients.
The discriminator base influences tRNA structure at the end of the acceptor stem and possibly its interaction with proteins. (1993)

For many tRNAs, the discriminator base preceding the CCA sequence at the 3' end is important for aminoacylation. We show that the discriminator base influences the stability of the 1.72 base pair onto which it is stacked. Mutations of the discriminator base from adenosine to cytidine or uridine make the cytidine residue in the C1-G72 base pair of mutant Escherichia coli initiator tRNAs more reactive toward sodium bisulfite, the single-strand-specific reagent. The activity of the enzyme Met-tRNA transformylase toward these and other mutant initiator tRNAs is also consistent with destabilization of the 1.72 base pair in vitro and in vivo. By influencing the strength of the 1.72 base pair, the discriminator base could affect the energetic cost of opening the base pair and modulate the structure of the tRNA near the site of aminoacylation. For some aminoacyl-tRNA synthetases and other proteins that interact with tRNA, these factors could be important for specific recognition and/or formation of the transition state during catalysis.
High Surface Area, sp2-Cross-Linked Three-Dimensional Graphene Monoliths (2011)

Developing three-dimensional (3D) graphene assemblies with properties similar to those individual graphene sheets is a promising strategy for graphene-based electrodes. Typically, the synthesis of 3D graphene assemblies relies on van der Waals forces for holding the graphene sheets together, resulting in bulk properties that do not reflect those reported for individual graphene sheets. Here, we report the use of sol-gel chemistry to introduce chemical bonding between the graphene sheets and control the bulk properties of graphene-based aerogels. Adjusting synthetic parameters allows a wide range of control over surface area, pore volume, and pore size, as well as the nature of the chemical cross-links (sp(2) vs sp(3)). The bulk properties of the graphene-based aerogels represent a significant step toward realizing the properties of individual graphene sheets in a 3D assembly with surface areas approaching the theoretical value of an individual sheet.
Preclinical Evaluation of the Safety and Immunogenicity of a Vaccine Consisting ofPlasmodium falciparumLiver-Stage Antigen 1 with Adjuvant AS01B Administered Alone or Concurrently with the RTS,S/AS01B Vaccine in Rhesus Primates (2007)

Several lines of evidence suggest that targeting pre-erythrocytic-stage parasites for malaria vaccine development can provide sterile immunity. The objectives of this study were (i) to evaluate preclinically the safety and immunogenicity of a new recombinant pre-erythrocytic-stage antigen, liver-stage antigen 1 (LSA1), in nonhuman primates; and (ii) to investigate the potential for immune interference between LSA1 and the leading malaria vaccine candidate, RTS,S, by comparing the immune responses after single-antigen vaccination to responses after simultaneous administration of both antigens at separate sites. Using a rhesus monkey model, we found that LSA1 formulated with the GlaxoSmithKline proprietary adjuvant system AS01B (LSA1/AS01B) was safe and immunogenic, inducing high titers of antigen-specific antibody and CD4+ T-cell responses, as monitored by the production of interleukin-2 and gamma interferon, using intracellular cytokine staining. RTS,S/AS01B vaccination was well tolerated and demonstrated robust antibody and moderate CD4+ T-cell responses to circumsporozoite protein (CSP) and HBsAg. Positive CD8+ T-cell responses to HBsAg were detected, whereas the responses to CSP and LSA1 were negligible. For both LSA1/AS01B and RTS,S/AS01B, no statistically significant differences were observed between individual and concurrent administration in the magnitude or duration of antibody and T-cell responses. Our results revealed that both pre-erythrocytic-stage antigens were safe and immunogenic, administered either separately or simultaneously to rhesus monkeys, and that no significant immune cross interference occurred with concurrent separate-site administration. The comparison of the profiles of immune responses induced by separate-site and single-site vaccinations with LSA1 and RTS,S warrants further investigation.
Study of the Dislocation Processes Associated with Strain Relaxation in Si - Ge Heteroepitaxial Films (1993)

Article Study of the Dislocation Processes Associated with Strain Relaxation in Si - Ge Heteroepitaxial Films was published on December 1, 1993 in the journal International Journal of Materials Research (volume 84, issue 12).
The signal sequence of an Escherichia coli outer membrane protein can mediate translocation of a not normally secreted protein across the plasma membrane. (1987)

The distal part of the long tail fibers of the Escherichia coli phage T4 consists of a dimer of protein 37. A fragment of the corresponding gene, encoding 253 amino acids, was inserted into several different sites within the cloned gene for the 325-residue outer membrane protein OmpA. In plasmid pTU T4-5 the fragment was inserted once and in pTU T4-10 tandemly twice between the codons for residues 153 and 154 of the OmpA protein. In pTU T4-22 two fragments were present, in tandem, between the codons for residues 45 and 46 of this protein. In pIN T4-6 one fragment was inserted into the ompA gene immediately following the part encoding the signal sequence. The corresponding mature proteins consist, in this order, of 605, 860, 835, and 279 amino acid residues. All precursor proteins were processed and translocated across the plasma membrane. Hence, not only can the OmpA protein serve as a vehicle for export of a nonsecretory protein, but the signal sequence alone can also mediate export of such a protein. Export of the pro-OmpA protein depends on the SecA protein. Export of the tail fiber fragment expressed from pIN T4-6 remained SecA dependent. Thus, the secA pathway in this case is chosen by the signal peptide. It is proposed that a signal peptide can mediate translocation of nonsecretory proteins as long as they are export-compatible. The inability of a signal sequence to mediate export of some proteins appears to be due to export incompatibility of the protein rather than to the absence of information, within the mature part of the polypeptide, which would be required for translocation.
Stimulated Emission Depletion Microscopy Resolves Individual Nitrogen Vacancy Centers in Diamond Nanocrystals (2013)

Nitrogen-vacancy (NV) color centers in nanodiamonds are highly promising for bioimaging and sensing. However, resolving individual NV centers within nanodiamond particles and the controlled addressing and readout of their spin state has remained a major challenge. Spatially stochastic super-resolution techniques cannot provide this capability in principle, whereas coordinate-controlled super-resolution imaging methods, like stimulated emission depletion (STED) microscopy, have been predicted to fail in nanodiamonds. Here we show that, contrary to these predictions, STED can resolve single NV centers in 40-250 nm sized nanodiamonds with a resolution of ≈10 nm. Even multiple adjacent NVs located in single nanodiamonds can be imaged individually down to relative distances of ≈15 nm. Far-field optical super-resolution of NVs inside nanodiamonds is highly relevant for bioimaging applications of these fluorescent nanolabels. The targeted addressing and readout of individual NV(-) spins inside nanodiamonds by STED should also be of high significance for quantum sensing and information applications.
Inertial levitation (2004)

We consider the steady levitation of a rigid plate on a thin air cushion with prescribed injection velocity. This injection velocity is assumed to be much larger than that in a conventional Prandtl boundary layer, so that inertial effects dominate. After applying the classical ‘blowhard’ theory of Cole &amp; Aroesty (1968) to the two-dimensional version of the problem, it is shown that in three dimensions the flow may be foliated into streamline surfaces using Lagrangian variables. An example is given of how this may be exploited to solve the three-dimensional problem when the injection pressure distribution is known.
Vibrational Spectrum of Trisdimethylaminocyclopropenium Perchlorate (1974)

Abstract Infrared spectrum in the region 4000–100 cm−1 and Raman spectrum of trisdimethylaminocyclopropenium perchlorate (1) have been measured. Band assignments have been made on the basis of normal coordinate analysis and deuteration of the methyl groups. Remarkable stabilization of cyclopropenium ion due to the peripheral amino groups is attributed to a marked delocalization of the positive charge of the ring on the amino groups through conjugation. Peculiar electronic structure of trisaminocyclopropenium ion is explained in terms of its vibrational spectrum.
Chirality transfer from optically active allylsilanes to π-allylpalladium complexes (1983)

The reaction of lithium palladates with the optically active allylsilanes, (E)- and(Z)-1-phenyl-1-trimethylsilyl-but-2-ene and (E)-and (z)-1-phenyl-3-trimethylsilylbut-1-ene proceeds stereospecifically [anti attack of palladium(II) with respect to the leaving silyl group] to give optically active π-allylpalladium complexes containing the η3-(1-methyl-3-phenylallyl) group in high yields.