gebhart/patrepeval huggingface dataset:
Difference in the expression of IL-9 and IL-17 correlates with different histological pattern of vascular wall injury in giant cell arteritis (2015)

Objective. GCA is a large- and medium-vessel arteritis characterized by a range of histological patterns of vascular wall injury. The aim of this study was to immunologically characterize the various histological patterns of GCA. Methods. Thirty-five consecutive patients with biopsy-proven GCA and 15 normal controls were studied. IL-8, IL-9, IL-9R, IL-17, IL-4, TGF-β and thymic stromal lymphopoietin expression was evaluated by RT–PCR and immunohistochemistry on artery biopsy specimens. Confocal microscopy was used to characterize the phenotypes of IL-9-producing and IL-9R-expressing cells. Five additional patients who had received prednisone when the temporal artery biopsy was performed were also enrolled to evaluate the effect of glucocorticoids on IL-9 and IL-17 expression. Results. IL-17 overexpression was observed mainly in arteries with transmural inflammation and vasa vasorum vasculitis. IL-9 overexpression and Th9 polarization predominated in arteries with transmural inflammation and small-vessel vasculitis. The tissue expression of both IL-9 and IL-17 was correlated with the intensity of the systemic inflammatory response. IL-4, TGF-β and thymic stromal lymphopoietin, which are involved in the differentiation of Th9 cells, were overexpressed in arteries with transmural inflammation and small-vessel vasculitis. IL-9R was also overexpressed in GCA arteries with transmural inflammation and was accompanied by increased expression of IL-8. Conclusion. Herein we provide the first evidence that distinct populations of potentially autoreactive T cells, expressing different cytokines (Th17 vs Th9), characterize patients with particular histological subsets of GCA and may thus contribute to the heterogeneity of tissue lesions observed in these patients.
Analysis and Optimization of Switched-Capacitor DC–DC Converters (2008)

Analysis methods are developed that fully determine a switched-capacitor (SC) dc-dc converter's steady-state performance through evaluation of its output impedance. This analysis method has been verified through simulation and experimentation. The simple formulation developed permits optimization of the capacitor sizes to meet a constraint such as a total capacitance or total energy storage limit, and also permits optimization of the switch sizes subject to constraints on total switch conductances or total switch volt-ampere (V-A) products. These optimizations then permit comparison among several switched-capacitor topologies, and comparisons of SC converters with conventional magnetic-based dc-dc converter circuits, in the context of various application settings. Significantly, the performance (based on conduction loss) of a ladder-type converter is found to be superior to that of a conventional magnetic-based converter for medium to high conversion ratios.
On the design of behavior-based multi-robot teams (1995)

Real-world applications that are ideal for robotic solutions are very complex and challenging. Many of these applications are set in dynamic environments that require capabilities distributed in functionality, space or time. These applications, therefore, often require teams of robots to work together cooperatively to successfully address the mission. While much research in recent years has addressed the issues of autonomous robots and multi-robot cooperation, current robotics technology is still far from achieving many of these real world applications. We believe that two primary reasons for this technology gap are that (1) previous work has not adequately addressed the issues of fault tolerance and adaptivity in multi-robot teams, and (2) existing robotics research is often geared at specific applications and is not easily generalized to different, but related, applications. This paper addresses these issues by first describing the design issues of key importance in these real-world cooperative robotics applications - fault tolerance, reliability, adaptivity and coherence. We then present a general architecture addressing these design issues - called ALLIANCE - that facilitates multi-robot cooperation of small- to medium-sized teams in dynamic environments, performing missions composed of loosely coupled subtasks. We illustrate the generality of this architecture by describing two very different proof-of-concept implementations of this architecture: a janitorial service mission and a bounding overwatch mission.
Feature interactions in services for Internet personal appliances (2003)

This paper investigates the feature interaction problem, known from traditional telephony environments, in the context of Internet personal appliances (IPA). IPAs are dedicated consumer devices which contain at least one network processor. They include the Internet alarm clock, which takes into account road conditions or expected arrival times of air planes when setting the alarm time, or the Internet enabled fridge, which keeps an inventory of groceries and issues orders to suppliers. The results of our investigation are threefold. The first part of this paper introduces a service taxonomy supported by a list of example services. The second part discusses feature interactions between services for appliances. A classification for such interactions and example interactions and possible conflicts are presented. The final part contains an outline for an approach to handle such interactions.
PLZT electrooptic shutters: applications (1975)

This paper describes advances in the development of several electrooptic shutter devices utilizing the quadratic electrooptic effect of lead lanthanum zirconate titanate (PLZT) ceramic wafers. Aperture sizes utilized in these PLZT devices ranged from 25 microm to 0.25 m. Practical applications of the shutters discussed in this paper include eye protection in military and industrial applications, a goggle-type device with dual synchronously operated PLZT shutters for use in a stereoscopic three-dimensional TV display, an electrically controlled variable density filter for use with vidicon tubes, a large-aperture photographic shutter for image motion compensation cameras, and a page composer for use in a holographic memory system.
5' flanking and first intron sequences of the human β-actin gene required for efficient promoter activity (1989)

We have identified a CCAAT box element that is required for the efficient transcription of the human beta-actin gene. Both in vivo transient transfection assays in cultured HeLa cells and in vitro run-off transcription assays in HeLa whole cell extracts demonstrated the requirement of this element for efficient promoter activity. A gel mobility shift assay revealed a Hela nuclear factor that specifically interacted with the beta-actin CCAAT element in vitro; mutation of the first three base pairs of the CCAAT pentanucleotide abolished binding of this factor. Competition gel shift experiments revealed that three sequence elements located within the beta-actin promoter, each containing a CC(A/T)6GG motif similar to that contained within the c-fos serum response element, were able to bind a different nuclear factor, serum response factor (SRF). One of these CC(A/T)6GG motifs is contained within a first intron fragment that enhanced transcription from a heterologous promoter in vivo.
Paradoxical glomerular filtration of carbon nanotubes (2010)

The molecular weight cutoff for glomerular filtration is thought to be 30-50 kDa. Here we report rapid and efficient filtration of molecules 10-20 times that mass and a model for the mechanism of this filtration. We conducted multimodal imaging studies in mice to investigate renal clearance of a single-walled carbon nanotube (SWCNT) construct covalently appended with ligands allowing simultaneous dynamic positron emission tomography, near-infrared fluorescence imaging, and microscopy. These SWCNTs have a length distribution ranging from 100 to 500 nm. The average length was determined to be 200-300 nm, which would yield a functionalized construct with a molecular weight of approximately 350-500 kDa. The construct was rapidly (t(1/2) approximately 6 min) renally cleared intact by glomerular filtration, with partial tubular reabsorption and transient translocation into the proximal tubular cell nuclei. Directional absorption was confirmed in vitro using polarized renal cells. Active secretion via transporters was not involved. Mathematical modeling of the rotational diffusivity showed the tendency of flow to orient SWCNTs of this size to allow clearance via the glomerular pores. Surprisingly, these results raise questions about the rules for renal filtration, given that these large molecules (with aspect ratios ranging from 100:1 to 500:1) were cleared similarly to small molecules. SWCNTs and other novel nanomaterials are being actively investigated for potential biomedical applications, and these observations-that high aspect ratio as well as large molecular size have an impact on glomerular filtration-will allow the design of novel nanoscale-based therapeutics with unusual pharmacologic characteristics.
2-[[(p-Nitrophenyl)sulfonyl]oxy] 3-Keto Esters as Intermediates for the Regiospecific Preparation of 2-[[(p-Nitrophenyl)sulfonyl]oxy] Ketones (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT2-[[(p-Nitrophenyl)sulfonyl]oxy] 3-Keto Esters as Intermediates for the Regiospecific Preparation of 2-[[(p-Nitrophenyl)sulfonyl]oxy] KetonesRobert V. Hoffman, Hwa-Ok Kim, and Jong Chan LeeCite this: J. Org. Chem. 1994, 59, 7, 1933–1936Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/jo00086a060https://doi.org/10.1021/jo00086a060research-articleACS PublicationsRequest reuse permissionsArticle Views185Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evidence for non‐oxidative dopamine cytotoxicity: potent activation of NF‐κ B and lack of protection by anti‐oxidants (2001)

A stable aromatic acid decarboxylase expressing the Chinese hamster ovary cell line was developed to study the cytotoxic properties of intracellular and extracellular dopamine. The relative impermeability of cells to dopamine, but not to L-DOPA, allows the differentiation of extracellular and intracellular dopamine cytotoxicity. In contrast to extracellular dopamine, intracellular dopamine toxicity was resistant to antioxidant protection, and did not require melanin formation for its toxicity. Furthermore, we demonstrated a rapid and potent activation of the stress-inducible transcription factor NF-kappa B by intracellular dopamine, which was also largely insensitive to antioxidant inhibition. A distinctly slower and less potent NF-kappa B activation by extracellular dopamine was blocked by antioxidants and acetylsalicylic acid. Our results indicate the existence of a non-oxidative mechanism of dopamine cytotoxicity. Mitigating intracellular dopamine toxicity could be a novel strategy of slowing the progressive degeneration of dopaminergic neurons in Parkinson's disease.
Crystal Clots as Therapeutic Target in Cholesterol Crystal Embolism (2020)

CC embolism causes arterial obstructions and organ failure via the formation of crystal clots with fibrin, platelets, and extracellular DNA as critical components. Therefore, our model enables to unravel the pathogenesis of the CC embolism syndrome as a basis for both prophylaxis and targeted therapy.
Biologic therapy for autoimmune diseases: an update (2013)

Biologic therapies for rheumatologic diseases, which are targeted at molecules involved in the mechanisms of the immune system, provide an alternative to the existing treatment methods of disease-modifying anti-rheumatic drugs and other immunosuppressive medications. However, the current drawbacks of biologic therapies, including the inconvenience of intravenous administration, the high costs of these drugs, and the adverse events associated with them, prevent their wide use as first-line medications. This review provides an update of the recent literature on the new biologic therapies available. The review concentrates on nine drugs: tocilizumab, rituximab, ofatumumab, belimumab, epratuzumab, abatacept, golimumab, certolizumab, and sifalimumab, which are used as therapies for rheumatoid arthritis, spondyloarthritis, systemic lupus erythematosus, systemic sclerosis, or vasculitis.
Poly(ethylene imine)s as Antimicrobial Agents with Selective Activity (2012)

We report the structure-activity relationship in the antimicrobial activity of linear and branched poly(ethylene imine)s (L- and B-PEIs) with a range of molecular weights (MWs) (500-12,000). Both L- and B-PEIs displayed enhanced activity against Staphylococcus aureus over Escherichia coli. Both B- and L-PEIs did not cause any significant permeabilization of E. coli cytoplasmic membrane. L-PEIs induced depolarization of S. aureus membrane although B-PEIs did not. The low MW B-PEIs caused little or no hemolysis while L-PEIs are hemolytic. The low MW B-PEIs are less cytotoxic to human HEp-2 cells than other PEIs. However, they induced significant cell viability reduction after 24 h incubation. The results presented here highlight the interplay between polymer size and structure on activity.
Induction of insulin secretion in engineered liver cells by nitric oxide (2007)

Liver cells can be engineered to produce insulin and insulin secretion can be induced by treatment with L-arginine via the production of nitric oxide.
The Current State of Interlanguage (1995)

This state-of-the-art volume presents an outstanding collection of 22 studies on current issues facing research in second-language acquisition (SLA). The editors sought contributions for this volume from seasoned veterans of SLA like Lydia White and Susan Gass, from well-known researchers in linguistics and/or first-language acquisition like Haj Ross and Harald Clahsen, and from relative newcomers to the field like India Plough and Jean-Marc Dewaele. The topics covered range from the role of universals at various levels of second-language (L2) knowledge; the way that linguistic knowledge is represented by L2 learners; the changing nature of linguistic theory itself; and the definition of usage phenomena like style shifting and code switching. The introduction to The Current State of Interlanguage gives a concise yet detailed overview of research in the field over the past 10 years, and focuses on the present growing concensus on a number of issues that were at one point highly controversial.
Spleen tyrosine kinase inhibition in the treatment of autoimmune, allergic and autoinflammatory diseases (2010)

Spleen tyrosine kinase (Syk) is involved in the development of the adaptive immune system and has been recognized as being important in the function of additional cell types, including platelets, phagocytes, fibroblasts, and osteoclasts, and in the generation of the inflammasome. Preclinical studies presented compelling evidence that Syk inhibition may have therapeutic value in the treatment of rheumatoid arthritis and other forms of arthritis, systemic lupus erythematosus, autoimmune cytopenias, and allergic and autoinflammatory diseases. In addition, Syk inhibition may have a place in limiting tissue injury associated with organ transplant and revascularization procedures. Clinical trials have documented exciting success in the treatment of patients with rheumatoid arthritis, autoimmune cytopenias, and allergic rhinitis. While the extent and severity of side effects appear to be limited so far, larger studies will unravel the risk involved with the clinical benefit.
Turbulent fluid shear stress induces vascular endothelial cell turnover in vitro. (1986)

The effects of hemodynamic forces upon vascular endothelial cell turnover were studied by exposing contact-inhibited confluent cell monolayers to shear stresses of varying amplitude in either laminar or turbulent flow. Laminar shear stresses (range, 8-15 dynes/cm2; 24 hr) induced cell alignment in the direction of flow without initiating the cell cycle. In contrast, turbulent shear stresses as low as 1.5 dynes/cm2 for as short a period as 3 hr stimulated substantial endothelial DNA synthesis in the absence of cell alignment, discernible cell retraction, or cell loss. The results of these in vitro experiments suggest that in atherosclerotic lesion-prone regions of the vascular system, unsteady blood flow characteristics, rather than the magnitude of wall shear stress per se, may be the major determinant of hemodynamically induced endothelial cell turnover.
Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network† (1998)

We have visualized the relationship between the endoplasmic reticulum (ER) and Golgi in leaf cells of Nicotiana clevelandii by expression of two Golgi proteins fused to green fluorescent protein (GFP). A fusion of the transmembrane domain (signal anchor sequence) of a rat sialyl transferase to GFP was targeted to the Golgi stacks. A second construct that expressed the Arabidopsis H/KDEL receptor homologue aERD2, fused to GFP, was targeted to both the Golgi apparatus and ER, allowing the relationship between these two organelles to be studied in living cells for the first time. The Golgi stacks were shown to move rapidly and extensively along the polygonal cortical ER network of leaf epidermal cells, without departing from the ER tubules. Co-localization of F-actin in the GFP-expressing cells revealed an underlying actin cytoskeleton that matched precisely the architecture of the ER network, while treatment of cells with the inhibitors cytochalasin D and N-ethylmaleimide revealed the dependency of Golgi movement on actin cables. These observations suggest that the leaf Golgi complex functions as a motile system of actin-directed stacks whose function is to pick up products from a relatively stationary ER system. Also, we demonstrate for the first time in vivo brefeldin A-induced retrograde transport of Golgi membrane protein to the ER.
A personal news agent that talks, learns and explains (1999)

Most work on intelligent information agents has thus far focused on systems that are accessible through the World Wide Web. As demanding schedules prohibit people from continuous access to their computers, there is a clear demand for information systems that do not require workstation access or graphical user interfaces. We present a personal news agent that is designed to become part of an intelligent, IP-enabled radio, which uses synthesized speech to read news stories to a user. Based on voice feedback from the user, the system automatically adapts to the user's preferences and interests. In addition to time-coded feedback, we explore two components of the system that facilitate the automated induction of accurate interest profiles. First, we motivate the use of a multistrategy machine learning approach that allows for the induction of user models that consist of separate models for long-term and short-term interests. Second, we investigate the use of "concept feedback", a novel form of user feedback that is based on our agent's capability to construct explanations for the reasons that have led to a specific classification. Users can then critique these explanations which, from a machine learning perspective, allows for more direct changes to an induced concept than through the inclusion of additional training examples. We evaluate the proposed algorithms on user data collected with a prototype of our system, and assess the performance contributions of the system's individual components.
Share and share alike (2006)

With the rapid growth of personal computer networks and the Internet, sharing files has become a central activity in computer use. The ways in which users control the what, how, and with whom of sharing are dictated by the tools they use for sharing; there are a wide range of sharing practices, and hence a wide range of tools to support these practices. In practice, users' requirements for certain sharing features may dictate their choice of tool, even though the other affordances available through that tool may not be an ideal match to the desired manner of sharing.In this paper, we explore users' current practices in file sharing and examine the tools used to share files. Based on our findings, we unpack the features and affordances of these tools into a set of dimensions along which sharing tools can be characterized. Then, we present the set of user interface features we have prototyped in an interface called a sharing palette, which provides a platform for exploration and experimentation with new modalities of sharing. We briefly present the tool as a whole and then focus on the individual features of the sharing palette that support reported styles of sharing.
Efficient selective frame discard algorithms for stored video delivery across resource constrained networks (1999)

Video delivery from a server to a client across a network is an important component of many multimedia applications. While delivering a video stream across a resource constrained network, loss of frames may be unavoidable. Under such circumstances, it is desirable to find a server transmission schedule that can efficiently utilize the network resources while maximizing the perceived quality-of-service (QoS) at the client. To address this issue, we introduce the notion of selective frame discard at the server and formulate the optimal selective frame discard problem using a QoS based cost function. Given network bandwidth and client buffer constraints, we develop an O(N log N) algorithm to find the minimum number of frames that must be discarded in order to meet these constraints. The correctness of the algorithm is also formally established. Since the computational complexity of the optimal algorithm for solving the optimal selective frame discard problem is prohibitively high in general, we also develop several efficient heuristic algorithms for selective frame discard. These algorithms are evaluated using JPEG video traces.
Oncolytic Immunotherapy: Where Are We Clinically? (2014)

Following a century of preclinical and clinical work, oncolytic viruses are now proving themselves in randomized phase 3 trials. Interestingly, human data indicates that these agents have potent immunostimulatory activity, raising the possibility that the key consequence of oncolysis might be induction of antitumor immunity, especially in the context of viruses harboring immunostimulatory transgenes. While safety and efficacy of many types of oncolytic viruses, including adenovirus, herpes, reo, and vaccinia seem promising, few mechanisms of action studies have been performed with human substrates. Thus, the relative contribution of "pure" oncolysis, the immune response resulting from oncolysis, and the added benefit of adding a transgene remain poorly understood. Here, the available clinical data on oncolytic viruses is reviewed, with emphasis on immunological aspects.