gebhart/patrepeval huggingface dataset:
Design and Implementation of Digital Radio Communications Link for Platoon Control Experiments (1995)

This report discusses the design and implementation of inter-vehicle communication systems used in the longitudinal control platoon experiments conducted by PATH (Partners for Advanced Transit and Highways). In the first section of the report, the inter-vehicle communication links implemented in both the two and four vehicles platoon experiments are examined. Lastly, this report investigates some possible future improvements of the communication link used for platoon control experimentation.
Specific information concerning taxonomy, pathogenicity and methicillin resistance of staphylococci obtained by a multiplex PCR (1997)

The use of DNA amplification techniques such as the polymerase chain reaction (PCR) in modern diagnostic microbiology not only allows the sensitive and specific identification of micro-organisms but also the detection of specific antibiotic resistance genes. This study describes a multiplex PCR on bacterial colonies picked directly from agar plates without preceding DNA preparation. Eubacteria and staphylococci were identified by, 16S rRNA specific PCR products. In parallel, specific primers were used for the detection of staphylococcal coa and mecA genes. This 4-h multiplex PCR, consisting of four sets of primers, was evaluated for rapid and specific differential diagnosis of methicillin-resistant and methicillin-susceptible strains of Staphylococcus aureus and coagulase-negative staphylococci. To analyse specificity of the amplification products, 100 non-staphylococcal, eubacterial isolates and 20 Candida albicans strains were tested. In a first step, specificity of all four single sets of primers was evaluated before the co-amplification within the multiplex PCR procedure was performed. The results were compared with those of conventional susceptibility and typing methods. The specific 16S rRNA PCR product for eubacterial isolates (n = 786) and staphylococci (686) was found in all strains tested. The coa gene was detected only in S. aureus (488) strains with a specificity of 100%, and was not detected in any of the coagulase-negative staphylococci (198). The mecA gene was detected in 98% of methicillin-resistant staphylococci (393) and in 2% of all methicillin-susceptible staphylococci (293). The multiplex PCR with co-amplification of different determinants provides rapid reliable information on staphylococcal identification and methicillin susceptibility supporting the diagnosis, treatment and control of staphylococcal infections.
Assay of Human Tissue-Type Plasminogen Activator (t-PA) with an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to t-PA (1985)

An enzyme-linked immunosorbent assay (ELISA) for the measurement of human tissue-type plasminogen activator (t-PA) was developed. Microtiter plates were coated with a mixture of two monoclonal antibodies and bound t-PA was quantitated with a third monoclonal antibody linked to peroxidase. The lower limit of sensitivity of the assay was 0.2 ng of t-PA per ml. The concentration of antigen in citrated plasma of human subjects was found to be 3.4 +/- 0.8 ng/ml. The assay had a good reproducibility with values of 3.8, 6.5 and 4.9 percent respectively for the intra-, inter-assay and inter-dilution variation coefficients. The results of the ELISA assay on plasma samples from patients during thrombolytic therapy with t-PA correlated very well, over a wide concentration range, with those obtained with a previously described two-site immuno-radiometric assay (r = 0.96). This ELISA with monoclonal antibodies constitutes a stable and reproducible set of reagents for the measurement of t-PA antigen in biological fluids, avoiding the disadvantages of the use of radioisotopes and of polyclonal antibodies.
Multibit Sigma - Delta A/D converter incorporating a novel class of dynamic element matching techniques (1992)

A dynamic element matching technique is applied to multibit sigma-delta modulators. The approach translates the harmonic distortion components of a nonideal digital-to-analog converter (DAC) in the feedback loop of a sigma-delta modulator to high-frequency components, which can then be filtered out by the decimation filter. Computer simulations have confirmed that with this approach a third-order sigma-delta modulator employing a 3-bit forward ADC, a 3-bit feedback DAC with a random mismatch of 0.1% can achieve a 104-dB (17+bit) dynamic range and a harmonic distortion below 100 dB, with an oversampling ratio of 64. The technique does not generate any tone in the passband.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Fiber-optic fluorosensor for oxygen and carbon dioxide (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFiber-optic fluorosensor for oxygen and carbon dioxideOtto S. Wolfbeis, Leonie J. Weis, Marc J. P. Leiner, and Werner E. ZieglerCite this: Anal. Chem. 1988, 60, 19, 2028–2030Publication Date (Print):October 1, 1988Publication History Published online1 May 2002Published inissue 1 October 1988https://pubs.acs.org/doi/10.1021/ac00170a009https://doi.org/10.1021/ac00170a009research-articleACS PublicationsRequest reuse permissionsArticle Views1225Altmetric-Citations207LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Chemical Free Room Temperature Wafer To Wafer Direct Bonding (1995)

A limitation to the use of direct wafer bonding methods for micromachining and thin film device manufacturing has been the necessity for high temperature anneals to strengthen the bonded interface. Obviously, strong interface strength is needed to withstand backthinning processes and the rigors of device fabrication. Unfortunately, the elevated temperature exposure has a detrimental effect on implanted or diffused etch stop layers via diffusive broadening. Additionally, for many micromachined applications wafer bonding could be used as a final assembly step, replacing epoxies. However, the sensitive components of the device must be protected from thermal effects. This paper describes the use of oxygen plasmas to develop chemical free, room temperature, wafer to wafer bonding methods. The bond developed between plasma‐activated silicon wafers is virtually at full strength upon contact bonding and does not require further thermal strengthening. The results for silicon dioxide bonding show that full strength material is achieved with anneals below 300°C. This process has been applied to a number of wafer materials including sapphire, silicon dioxide, silicon nitride, and gallium arsenide. The data presented are the results of strength tests, interfacial defect etching, transmission electron microscopy analysis, initial interface reaction kinetics, and mechanisms studies. We also show preliminary results from a suggested model to explain the observed increases in kinetics compared to conventional aqueous solution processing of samples.
Partial amino-acid sequence of the precursor of an immunoglobulin light chain containing NH2-terminal pyroglutamic acid. (1976)

Analyses of amino-acid sequences of the total cell-free products programmed by the mRNA of MOPC-104E gamma light (L)-chain show that over 95% of the products have sequences of a distinct protein that correspond to the L-chain precursor. In this precursor an extra piece is coupled to the NH2-terminus of the mature L-chain. Analyses of products labeled with [3H]alanine, [3H]leucine, and [3H]proline demonstrate that the extra piece is composed of at least 18 residues. Analyses of [35S]methione-labeled product indicate that the extra piece may contain an additional NH2-terminal methionine, which is detected in about 10% of the molecules. Partial recovery of the NJ2-terminal methionine (alanine, leucine, and proline are recovered in yields close to theoretical, greater than 95%) suggests that it is the initiator methionine, which is known to be short lived in eukaryotes due to rapid hydrolysis. Thus, the extra piece seems to be 19 residues in length, and it contains one methionine at the NH2-terminus, three alanines at positions 2, 12, and 17, and five leucines at positions 6, 8, 10, 11, and 13. The close gathering of leucine residues, as well as their abundance (26%), suggest that the extra piece would be quite hydrophobic. Hydrophobicity seems to be a general property of the extra piece, since similar clusters of leucine were found in the precursors of 3 KL-chains (Burstein, Y. & Schechter, I. (1976) Biochem. J. 157, 145-151). The NH2-terminus of the mature MOPC-104E gamma L-chain is blocked by pyroglutamic acid. The fact that in the precursor a peptide segment precedes this NH2-terminus establishes that pyroglutamic acid is not the initiator residue for synthesis of the L-chain. Apparently, the pyroglutamic acid is formed by cyclization of glutamic acid or glutamine during cleavage of the extra piece to yield the mature L-chain.
Access 2002 Bible with CD-ROM (1997)

From the Publisher:
Access 97, the world's most popular database program is out at last -- and more feature-packed than ever before. With Access 97 Bible you'll discover just how feature-packed it is. Here, Access experts Cary N. Prague and Michael R. Irwin share the results of their rare and thorough prerelease examination. From designing, creating, and using Access 97 databases to merging them with other Office applications, generating time-saving reports, and automating routine tasks using Visual Basic, Access 97 Bible serves as the best possible single-source reference. Plus, on the book's accompanying bonus CD-ROM, you get indispensable resources and utilities, including Check Writer, a library of business forms, command bar images, all the examples from the book (ready for you to adapt for your own use), and two bonus chapters: An Introduction to VBA and The Access 97 Programming Environment.
MACROPHAGE COLONY STIMULATING FACTOR RESTORESIN VIVOBONE RESORPTION IN THEOP/OPOSTEOPETROTIC MOUSE (1990)

The op/op variant of murine osteopetrosis is a recessive mutation characterized by impaired bone resorption due to lack of osteoclasts. Cultured osteoblasts and fibroblasts from this mutant do not secrete M-CSF activity and resident macrophages are absent in bone marrow. This failure has been related to a mutation within the M-CSF coding region. We report now that the administration of recombinant human M-CSF (rhM-CSF) corrects in vivo the impaired bone resorption in this animal. The treatment restores the bone marrow cavity virtually absent in the op/op animal and induces the appearance of resorbing osteoclasts and of resident bone marrow macrophages. This proves that the deficiency of M-CSF is the cause of the op/op bone disorder and that this cytokine is directly or indirectly necessary for physiological osteoclastogenesis, the resulting bone resorption and for the establishment of bone marrow hemopoiesis.
Antibiotic Treatment ofChlamydia pneumoniaeafter Acute Coronary Syndrome (2005)

Despite long-term treatment with a bactericidal antibiotic effective against C. pneumoniae, no reduction in the rate of cardiovascular events was observed.
Scalable packet classification using distributed crossproducting of field labels (2005)

A wide variety of packet classification algorithms and devices exist in the research literature and commercial market. The existing solutions exploit various design tradeoffs to provide high search rates, power and space efficiency, fast incremental updates, and the ability to scale to large numbers of filters. There remains a need for techniques that achieve a favorable balance among these tradeoffs and scale to support classification on additional fields beyond the standard 5-tuple. We introduce distributed crossproducing of field labels (DCFL), a novel combination of new and existing packet classification techniques that leverages key observations of the structure of real filter sets and takes advantage of the capabilities of modern hardware technology. Using a collection of real and synthetic filter sets, we provide analyses of DCFL performance and resource requirements on filter sets of various sizes and compositions. An optimized implementation of DCFL can provide over 100 million searches per second and storage for over 200 thousand filters in a current generation FPGA or ASIC without the need for external memory devices.
Inhibitory, opsonic and cytotoxic activities of monoclonal antibodies against asexual erythrocytic stages ofPlasmodium falciparum (1987)

A range of monoclonal antibodies specific for Plasmodium falciparum were tested in vitro for their abilities to inhibit the multiplication of a partially synchronized culture of P. falciparum, to augment the phagocytosis of the parasites by macrophages, and to enhance the killing of parasites by peritoneal cells depleted of adherent cells. Seven of 17 monoclonal antibodies, ranging from culture supernatant fluid and ascitic fluid to purified IgG, showed dose- and time-dependent inhibition of parasite growth in vitro. At a concentration of 0.6 mg/ml, the inhibitory capacity of these monoclonal IgGs was above 94% over a 3-day culture period, much higher than that of the relevant polyclonal IgG. Four of 6 monoclonal antibodies tested augmented the phagocytosis of the parasites by macrophages, which occurred as a result of opsonization of the parasites. Four of 7 monoclonal antibodies examined showed cytotoxic activity on malaria parasites. Peritoneal cells depleted of adherent cells were capable of killing the parasites in the presence of monoclonal antibodies. These results indicate that there may be 'monofunction', 'bifunction', and 'multifunction' types of monoclonal antibodies against P. falciparum. The putative protective antigen of malaria parasites purified by 'multifunctional monoclonal antibody' affinity chromatography may have potential interest as a vaccine against the parasite or as an immunodiagnostic reagent for human malaria.
Human cytotoxic T cells stimulated by antigen on dendritic cells recognize the N, SH, F, M, 22K, and 1b proteins of respiratory syncytial virus (1992)

We examined the human cytotoxic T-cell repertoire of nine adults to 9 of the 10 proteins of respiratory syncytial (RS) virus. Peripheral blood mononuclear cells from normal adults were stimulated with RS virus in vitro. The resulting polyclonal cultures were tested for lysis of B-lymphoblastoid cell lines infected with recombinant vaccinia viruses expressing each of nine individual RS virus proteins. The use of peripheral blood dendritic cells to present antigen gave more easily reproducible results over a shorter culture period than conventional methods. The six RS virus proteins most strongly recognized were the nucleoprotein N (nine of nine donors with greater than 10% above background lysis; P = 0.0004), the surface proteins SH (six of nine donors; P = 0.002) and F (five of nine donors; P = 0.008), the matrix proteins M (five of nine donors; P = 0.004) and 22K (three of nine donors; P = 0.01) and the nonstructural protein 1b (six of nine donors; P = 0.004). There was no significant recognition of the major surface glycoprotein G (two of nine donors), the internal phosphoprotein P (one of nine donors), or the nonstructural protein 1c (one of nine donors). Recognition was major histocompatibility complex class I restricted, but no association between major histocompatibility complex phenotype and protein specificity of T cells was seen. Recognition of F and 22K appeared to be associated with recent infection indicated by increased levels of anti-RS virus immunoglobulin G antibody in serum measured by enzyme-linked immunosorbent assay. Since cytotoxic T-cell recognition of RS virus proteins has been demonstrated to be important in the clearance of virus from infected hosts, the N, M, SH, 1b, F, and 22K proteins should be considered potential vaccine components.
A History of the INTERNIST-1 and Quick Medical Reference (QMR) Computer-Assisted Diagnosis Projects, with Lessons Learned (2010)

The INTERNIST-1/Quick Medical Reference (QMR) diagnostic decision support project spans four decades, from 1971-onward. This paper describes the history of the project and details insights gained of relevance to the general clinical and informatics communities.
The Symptomatic Effect of Cisapride in Patients with Irritable Bowel Syndrome and Constipation (1998)

The trial seems to exclude a clinically significant effect of 15-30 mg cisapride daily in patients with IBS and constipation during a 12-week treatment period.
Cannabinoids and neuroinflammation (2004)

Growing evidence suggests that a major physiological function of the cannabinoid signaling system is to modulate neuroinflammation. This review discusses the anti-inflammatory properties of cannabinoid compounds at molecular, cellular and whole animal levels, first by examining the evidence for anti-inflammatory effects of cannabinoids obtained using in vivo animal models of clinical neuroinflammatory conditions, specifically rodent models of multiple sclerosis, and second by describing the endogenous cannabinoid (endocannabinoid) system components in immune cells. Our aim is to identify immune functions modulated by cannabinoids that could account for their anti-inflammatory effects in these animal models.
Low-rank matrix factorization for Deep Neural Network training with high-dimensional output targets (2013)

While Deep Neural Networks (DNNs) have achieved tremendous success for large vocabulary continuous speech recognition (LVCSR) tasks, training of these networks is slow. One reason is that DNNs are trained with a large number of training parameters (i.e., 10–50 million). Because networks are trained with a large number of output targets to achieve good performance, the majority of these parameters are in the final weight layer. In this paper, we propose a low-rank matrix factorization of the final weight layer. We apply this low-rank technique to DNNs for both acoustic modeling and language modeling. We show on three different LVCSR tasks ranging between 50–400 hrs, that a low-rank factorization reduces the number of parameters of the network by 30–50%. This results in roughly an equivalent reduction in training time, without a significant loss in final recognition accuracy, compared to a full-rank representation.
Sporozoites of Rodent and Simian Malaria, Purified by Anion Exchangers, Retain their Immunogenicity and Infectivity (1978)

Sporozoites of rodent malaria, Plasmodium berghei, and simian malaria, Plasmodium knowlesi and Plasmodium cynomolgi, were partially separated from mosquito debris and microbial contaminants by passage of Anopheles material through a DEAE-cellulose column. In addition to eliminating most of the contaminants (80-90%), this simple technic has made it possible to recover rapidly large numbers of viable sporozoites (55-75% yield), which have retained their infectivity, immunogenicity, and capacity to react with known antisera. Mice injected with varying doses of column-purified sporozoites (CS) of P. berghei produced infections which paralleled those seen in the controls. Total protection against challenge with a potentially lethal dose of viable sporozoites was acquired by mice inoculated twice with irradiated CS of P. berghei CS of P. berghei and P. cynomolgi gave positive circumsporozoite precipitation (CSP) reactions, upon inoculation with the respective immune sera. The preservation of the surface antigens of CS was documented by immunofluorescence. It was shown that differences in elution behavior exist among sporozoites of certain species of Plasmodium as well as among sporozoiters of the same species derived from different organs of the mosquito. These results may be attributed to differences in the surface charge of the sporozoites or conditions in sample media. Purified sporozoites obtained by the method described in this report provide an adequate source of parasites for a variety of in vitro studies.
The cytoplasmic domain of CD4 promotes the development of CD4 lineage T cells. (1996)

Thymocytes must bind major histocompatibility complex (MHC) proteins on thymic epithelial cells in order to mature into either CD8+ cytotoxic T cells or CD4+ helper T cells. Thymic precursors express both CD8 and CD4, and it has been suggested that the intracellular signals generated by CD8 or CD4 binding to class I or II MHC, respectively, might influence the fate of uncommitted cells. Here we test the notion that intracellular signaling by CD4 directs the development of thymocytes to a CD4 lineage. A hybrid protein consisting of the CD8 extracellular and transmembrane domains and the cytoplasmic domain of CD4 (CD884) should bind class I MHC but deliver a CD4 intracellular signal. We find that expression of a hybrid CD884 protein in thymocytes of transgenic mice leads to the development of large numbers of class I MHC-specific, CD4 lineage T cells. We discuss these results in terms of current models for CD4 and CD8 lineage commitment.
The B cell antigen CD75 is a cell surface sialytransferase. (1990)

In this work we have isolated a cDNA clone encoding the B cell antigen CD75. The amino acid sequence of CD75 is shown to be identical to that of human alpha 2,6 sialyltransferase, believed to be primarily associated with the Golgi complex. This is the first demonstration of cell surface expression of sialytransferase which, in B cells, may play an important role in intercellular adhesion and antigen presentation events.
Modification of 15q11 — q13 DNA methylation imprints in unique Angelman and Prader — Willi patients (1993)

The clearest example of genomic Imprinting in humans comes from studies of the Angelman (AS) and Prader—Wil (PWS) syndromes. Although these are clinically distinct disorders, both typically result from a loss of the same chromosomal region, 15q11 - q13. AS usually results from either a maternal deletion of this region, or paternal uniparental disomy (UPD; both chromosomes 15 Inherited from the father). PWS results from paternal deletion of 15q11 - q13 or maternal UPD of chromosome 15. We have recently described a parent-specific DNA methylation imprint in a gene at the D15S9 locus (new gene symbol, ZNF 127), within the 15q11 - q13 region, that identifies AS and PWS patients with either a deletion or UPD. Here we describe an AS sibship and three PWS patients in which chromosome 15 rearrangements alter the methylation state at ZNF127, even though this locus is not directly involved in the rearrangement. Parent-specific DNA methylation imprints are also altered at ZNF127 and D15S63 (another locus with a parent-specific methylation imprint) in an AS sibship which have no detectable deletion or UPD of chromosome 15. These unique patients may provide insight into the imprinting process that occurs in proximal chromosome 15 in humans.