gebhart/patrepeval huggingface dataset:
Virtual wires: overcoming pin limitations in FPGA-based logic emulators (2002)

Existing FPGA-based logic emulators only use a fraction of potential communication bandwidth because they dedicate each FPGA pin (physical wire) to a single emulated signal (logical wire). Virtual wires overcome pin limitations by intelligently multiplexing each physical wire among multiple logical wires and pipelining these connections at the maximum clocking frequency of the FPGA. A virtual wire represents a connection from a logical output on one FPGA to a logical input on another FPGA. Virtual wires not only increase usable bandwidth, but also relax the absolute limits imposed on gate utilization. The resulting improvement in bandwidth reduces the need for global interconnect, allowing effective use of low dimension inter-chip connections (such as nearest-neighbor). Nearest-neighbor topologies, coupled with the ability of virtual wires to overlap communication with computation, can even improve emulation speeds. The authors present the concept of virtual wires and describe their first implementation, a 'softwire' compiler which utilizes static routing and relies on minimal hardware support. Results from compiling netlists for the 18 K gate Sparcle microprocessor and the 86 K gate Alewife Communications and Cache Controller indicate that virtual wires can increase FPGA gate utilization beyond 80 percent without a significant slowdown in emulation speed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Effects of fatty ethers and stearic acid on the gastrointestinal absorption of insulin. (1990)

Insulin degradation and inactivation occurs when the protein is given to an animal through oral route. The following compounds were assessed to determine their potential effect in increasing insulin absorption: polyoxyethylene (20) oleyl ether (Brij 99), polyoxyethylene lauryl ether and polyoxyethylene (20) cetyl ether (Brij 58). In this study the above compounds, along with stearic acid, were used to prepare granules with or without addition of insulin and were given orally to rabbits. Both glucose and insulin levels in blood were measured at different time intervals, the former with a glucometer and the latter using radioimmunoassay (RIA). To determine if there was a relationship between the glucose and the insulin levels in blood, a highly sensitive radioimmunoassay method was used to detect any small changes in the level of insulin in blood. A significant decrease in glucose blood levels (P less than 0.05) was obtained after oral administration of each of granules containing insulin. The greatest reduction was observed after half an hour of the oral administration of the granules. A similar response was obtained with the hypodermal injection of 1/5 of the oral insulin dose. Granules prepared with Brij 58 and containing insulin was the only formula that showed increase of insulin level in the blood. This was equivalent to 1/10 of the change produced after hypodermal injection of the hormone. In these experiments the changes in blood insulin levels were not directly proportional to the corresponding changes in blood glucose.
Relaxed acceptor site specificity of bacterial oligosaccharyltransferase in vivo (2010)

A number of proteobacteria carry the genetic information to perform N-linked glycosylation, but only the protein glycosylation (pgl) pathway of Campylobacter jejuni has been studied to date. Here, we report that the pgl gene cluster of Campylobacter lari encodes for a functional glycosylation machinery that can be reconstituted in Escherichia coli. We determined that the N-glycan produced in this system consisted of a linear hexasaccharide. We found that the oligosaccharyltransferase (OST) of C. lari conserved a predominant specificity for the primary sequence D/E-X(-1)-N-X(+1)-S/T (where X(-1) and X(+1) can be any amino acid but proline). At the same time, we observed that this enzyme exhibited a relaxed specificity toward the acceptor site and modified asparagine residues of a protein at sequences DANSG and NNNST. Moreover, C. lari pgl glycosylated a native E. coli protein. Bacterial N-glycosylation appears as a useful tool to establish a molecular description of how single-subunit OSTs perform selection of glycosyl acceptor sites.
Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO) (1996)

Electroporation is one of the most common methods used transform mammalian cells with plasmids. This method is versatile and can be adapted to meet the requirements of many cell lines. However, sometimes the efficiency of this method is low. We demonstrate that dimethyl sulfoxide (DMSO) facilitated a better DNA uptake in four different cell lines (HL60, TR146, Cos-7 and L132). The cells were electroporated with a beta-Gal expression plasmid in a medium containing DMSO (1.25%) during, and for 24 h after the pulse. In all these cells a dramatic (up to 8-fold) increase in transfection efficiency occurred after this treatment. This method opens up the possibility of using electroporation even in cells which are difficult to transfect.
A rapid and versatile method to synthesize internal standards for competitive PCR (1993)

Journal Article A rapid and versatile method to synthesize internal standards for competitive PCR Get access Francesco S. Celi, Francesco S. Celi The National Institute on Aging, National Institutes of Health and the Johns Hopkins University School of MedicineBaltimore, MD 21224, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael E. Zenilman, Michael E. Zenilman The National Institute on Aging, National Institutes of Health and the Johns Hopkins University School of MedicineBaltimore, MD 21224, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Alan R Shuldiner Alan R Shuldiner * The National Institute on Aging, National Institutes of Health and the Johns Hopkins University School of MedicineBaltimore, MD 21224, USA *To whom correspondence should be addressed at: Johns Hopkins University School of Medicine, 5501 Bayview Circle, Room 5A.42, Baltimore, MD 21224, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 21, Issue 4, 25 February 1993, Page 1047, https://doi.org/10.1093/nar/21.4.1047 Published: 25 February 1993 Article history Received: 21 December 1992 Accepted: 19 January 1993 Published: 25 February 1993
Comment on “Water harvesting from air with metal-organic frameworks powered by natural sunlight” (2017)

Kim <i>et al</i> (Reports, 28 April 2017, p. 430) presented results for the solar-driven harvesting of water from air via metal-organic frameworks as a prodigious potential advance toward remedying global water shortages. Basic thermodynamics and a survey of multiple off-the-shelf technologies show that their approach is vastly inferior in efficiency (and thereby in feasibility) to available alternatives.
MRI activity and neutralising antibody as predictors of response to interferon treatment in multiple sclerosis (2007)

MRI activity and NAb occurrence during the first 6 months of interferon beta treatment were reliable predictors of long term clinical response, particularly when combined. Patients with negative predictors showed a less than 10% risk of developing clinical activity. Patients with positive predictors showed a 50% risk of further clinical activity. These patients need to be followed carefully with further MRI and NAb tests.
Explicit identification and implicit recognition of facial emotions: II. Core domains and relationships with general cognition (2008)

Both general and social cognition are important in providing endophenotypic markers and predicting real-world functional outcomes of clinical psychiatric disorders. However, to date, focus has been on general cognition, rather than on core domains of social/emotional cognition. This study sought to determine core domains of emotion processing for both explicit identification and implicit recognition and their relationships with core domains of general cognition. Age effects and sex differences were also investigated. A sample of 1,000 healthy individuals (6 to 91 years, 53.5% female) undertook the WebNeuro tests of emotion identification and recognition and tests of general cognitive function. Factor analysis revealed seven core domains of emotion processing: speed of explicit emotion identification, speed of implicit emotion recognition, implicit emotion recognition accuracy, "threat" processing, sadness-disgust identification, "positive emotion" processing, and general "face perception." Seven corresponding core domains of general cognition were identified: information-processing speed, executive function, sustained attention/vigilance, verbal memory, working-memory capacity, inhibition/impulsivity, and sensorimotor function. Factors of emotion processing generally showed positive associations with those of general cognitive function, suggesting commonality in processing speed in particular. Moreover, age had a consistent nonlinear impact on both emotion processing and general cognitive factors, while sex differences were more specific. These findings contribute to a normative and standardized structure for assessment of emotional and general cognition in clinical groups.
A Linear Multi-Mode CMOS Power Amplifier With Discrete Resizing and Concurrent Power Combining Structure (2011)

Efficiency degradation effects of power combining transformers with partially disabled inputs are quantitatively analyzed. To improve efficiencies in lower-power modes of a multi-mode class-AB power amplifier (PA), a discrete resizing technique is introduced in combination with a parallel-combining transformer (PCT). The two-stage PA implemented in a 0.18-μm CMOS technology also includes varactor-based tunable matching circuits. The design method involves parallel-combining of two power stages, each of which are divided into three sub-cells to facilitate discrete resizing. The parallel-combining of concurrently resized power cells minimizes undesired power loss through the transformer and helps the PA to utilize the transformer efficiency maximally independent of the number of combining cells. When operating in the high-power mode, the PA exhibits a peak output power of 31 dBm with a PAE of 34.8%. Power back-offs are realized by discretely turning off parallel sub-amplifier cells concurrently, achieving output power levels of 26 dBm and 22.3 dBm with respective PAE of 22.5% and 15%. The EVM has been measured with IEEE 802.11g WLAN and 802.16e WiMAX modulated signals in three operation modes. In the high-power mode, the PA dissipates 590 mA from a 3.3 V supply.
Preparation and anti‐tumor efficiency evaluation of doxorubicin‐loaded bacterial magnetosomes: Magnetic nanoparticles as drug carriers isolated from Magnetospirillum gryphiswaldense (2008)

Bacterial magnetosomes (BMs) are commonly used as vehicles for certain enzymes, nucleic acids and antibodies, although they have never been considered drug carriers. To evaluate the clinical potential of BMs extracted from Magnetospirillum gryphiswaldense in cancer therapy, doxorubicin (DOX) was loaded onto the purified BMs at a ratio of 0.87 +/- 0.08 mg/mg using glutaraldehyde. The DOX-coupled BMs (DBMs) and BMs exhibited uniform sizes and morphology evaluated by TEM. The diameters of DBMs and BMs obtained by AFM were 71.02 +/- 6.73 and 34.93 +/- 8.24 nm, respectively. The DBMs released DOX slowly into serum and maintained at least 80% stability following 48 h of incubation. In vitro cytotoxic tests showed that the DBMs were cytotoxic to HL60 and EMT-6 cells, manifested as inhibition of cell proliferation and suppression in c-myc expression, consistent with DOX. These observations depicted in vitro antitumor property of DBMs similar to DOX. The approach of coupling DOX to magnetosomes may have clinical potential in anti-tumor drug delivery.
FEZ1/LZTS1 gene at 8p22 suppresses cancer cell growth and regulates mitosis (2001)

The FEZ1/LZTS1 gene maps to chromosome 8p22, a region that is frequently deleted in human tumors. Alterations in FEZ1/LZTS1 expression have been observed in esophageal, breast, and prostate cancers. Here, we show that introduction of FEZ1/LZTS1 into Fez1/Lzts1-negative cancer cells results in suppression of tumorigenicity and reduced cell growth with accumulation of cells at late S-G(2)/M stage of the cell cycle. Fez1/Lzts1 protein is hyperphosphorylated by cAMP-dependent kinase during cell-cycle progression. We found that Fez1/Lzts1 is associated with microtubule components and interacts with p34(cdc2) at late S-G(2)/M stage in vivo. Present data show that FEZ1/LZTS1 inhibits cancer cell growth through regulation of mitosis, and that its alterations result in abnormal cell growth.
Photoperiodic Pereception in Biloxi Soy Beans (1938)

1. When whole plants of Biloxi soy beans are subjected to 8 hour photoperiods, initiation occurs if the intensity of light during the photoperiod is above 100 foot candles. Below 100 foot candles no flower primordia are initiated. 2. When plants are given an 8 hour photoperiod of natural light supplemented by 8 hours of Mazda light, initiation occurs if the intensity of the supplemental light is below 0.5 foot candle, but does not occur if the intensity is above 0.5 foot candle. 3. The stimulus that causes initiation of flower primordia at the growing points arises in the leaves and moves to the growing plants. 4. Flower primordia may be initiated at growing points that are kept, either in complete darkness or on photoperiods above the critical, provided the leaves are kept on short photoperiods. 5. Photoperiods shorter than the critical, applied directly to growing points, have no effect on flower bud initiation. Control of initiation is exercised only through application of photoperiods of proper length to the leaves. 6. Whether the responses secured in these experiments are due to a flower forming hormone or to other causes remains to be determined.
A pilot clinical trial testing mutant von Hippel-Lindau peptide as a novel immune therapy in metastatic Renal Cell Carcinoma (2010)

98C0139.
A Novel Scheme for Wide Bandwidth Chip-to-Chip Communications (2007)

Quilt Packaging (QP), a novel chip-to-chip communication paradigm for system-in-package integration, is presented. By forming protruding metal nodules along the edges of the chips and interconnecting integrated circuits (ICs) through them, QP offers an approach to ameliorate the I/O speed bottleneck. A fabrication process that includes deep reactive ion etching, electroplating, and chemical-mechanical polishing is demonstrated. As a low-temperature process, it can be easily integrated into a standard IC fabrication process. Three-dimensional electromagnetic simulations of coplanar waveguide QP structures have been performed, and geometries intended to improve impedance matching at the interface between the on-chip interconnects and the chip-to-chip nodule structures were evaluated. Test chips with 100 μm wide nodules were fabricated on silicon substrates, and s-parameters of chip-to-chip interconnects were measured. The insertion loss of the chip-to-chip interconnects was as low as 0.2 dB at 40 GHz. Simulations of 20 μm wide QP structures suggest that the bandwidth of the inter-chip nodules is expected to be above 200 GHz.
Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain (1992)

An Escherichia coli strain has been constructed that produces the copolymer poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(HB-co-HV). This has been accomplished by placing the PHB biosynthetic genes from Alcaligenes eutrophus into an E. coli fadR atoC(Con) mutant and culturing the strain in M9 minimal medium containing glucose and propionate. 3-Hydroxyvalerate incorporation is absolutely dependent on the presence of both glucose and propionate, and 3-hydroxybutyrate-3-hydroxyvalerate ratios in the copolymer can be manipulated by altering the propionate concentration and/or the glucose concentration in the culture. P(HB-co-HV) production can be accomplished by using a wide variety of feeding regimens, but the most efficient is to allow the culture to grow to late log phase in minimal medium containing acetate and then add glucose and propionate to initiate copolymer production. A broad range of propionate concentrations can be used in the culture to stimulate 3-hydroxyvalerate incorporation; however, the most efficient utilization of propionate occurs at concentrations below 10 mM. 3-Hydroxyvalerate molar percentages in the copolymer are relatively constant over the course of growth. The copolymer has been purified and confirmed to be P(HB-co-HV) by gas chromatography/mass spectrometry and differential scanning calorimetry.
The herpes simplex virus 1 RNA binding protein US11 is a virion component and associates with ribosomal 60S subunits (1992)

The herpes simplex virus 1 US11 gene encodes a site- and conformation-specific RNA binding regulatory protein. We fused the coding sequence of this protein with that of beta-galactosidase, expressed the chimeric gene in Escherichia coli, and purified a fusion protein which binds RNA in the same way as the infected cell protein. The fusion protein was used to generate anti-US11 monoclonal antibody. Studies with this antibody showed that US11 protein is a viral structural protein estimated to be present in 600 to 1,000 copies per virion. The great majority of cytoplasmic US11 protein was found in association with the 60S subunit of infected cell ribosomes. US11 protein associates with ribosomes both late in infection at the time of its synthesis and at the time of infection after its introduction into the cytoplasm by the virion. US11 protein expressed in an uninfected cell line stably transfected with the US11 gene associates with ribosomal 60S subunits and localizes to nucleoli, suggesting that US11 protein requires no other viral functions for these associations.
Infliximab for maintenance of glucocorticosteroid-induced remission of giant cell arteritis: a randomized trial. (2007)

This trial is too small to draw definitive conclusions, but it provides evidence that using infliximab as maintenance therapy in patients in glucocorticoid-induced remission of newly diagnosed giant cell arteritis is of no benefit and may be harmful. If infliximab has benefit, it is unlikely to be great. ClinicalTrials.gov registration number: NCT00076726.
Mesenchymal Stem Cell Targeting of Microscopic Tumors and Tumor Stroma Development Monitored by Noninvasive In vivo Positron Emission Tomography Imaging (2005)

The aim of this study was to assess the efficacy human mesenchymal stem cells (hMSC) for targeting microscopic tumors and suicide gene or cytokine gene therapy. Immunodeficient mice were transplanted s.c. with human colon cancer cells of HT-29 Inv2 or CCS line, and 3 to 4 days later, i.v. with "tracer" hMSCs expressing herpes simplex virus type 1 thymidine kinase (HSV1-TK) and enhanced green fluorescent protein (EGFP) reporter genes. Subsequently, these tumors were examined for specificity and magnitude of HSV1-TK(+), EGFP(+) stem cell engraftment and proliferation in tumor stroma by in vivo positron emission tomography (PET) with (18)F-labeled 9-(4-fluoro-3-hydroxymethylbutyl)-guanine ([(18)F]-FHBG). In vivo PET images of tumors growing for 4 weeks showed the presence of HSV1-TK(+) tumor stroma with an average of 0.36 +/- 0.24% ID/g [(18)F]-FHBG accumulation. In vivo imaging results were validated by in situ correlative histochemical, immunofluorescent, and cytometric analyses, which revealed EGFP expression in vWF(+) and CD31(+) endothelial cells of capillaries and larger blood vessels, in germinal layer of dermis and hair follicles proximal to the s.c. tumor site. These differentiated HSV1-TK(+), GFP(+) endothelial cells had limited proliferative capacity and a short life span of <2 weeks in tumor fragments transplanted into secondary hosts. We conclude that hMSCs can target microscopic tumors, subsequently proliferate and differentiate, and contribute to formation of a significant portion of tumor stroma. PET imaging should facilitate clinical translation of stem cell-based anticancer gene therapeutic approaches by providing the means for in vivo noninvasive whole-body monitoring of trafficking, tumor targeting, and proliferation of HSV1-tk-expressing "tracer" hMSCs in tumor stroma.
Toward assessment of blood oxygen saturation by spectroscopic optical coherence tomography (2005)

The use of spectroscopic optical coherence tomography to assess hemoglobin oxygen saturation of whole blood is investigated. We propose to use the differential attenuation coefficient to determine the degree of saturation. Our data show qualitative agreement between the measured differential attenuation coefficients as a function of saturation and predictions based on the oxygen-saturation-dependent absorption and scattering properties of blood.
Evaluation of Surface Hydrophobicities of Proteins Using Hydrophobic Interaction with Non-ionic Surfactants in Aqueous Two-Phase Partitioning Systems. (1993)

タンパク質の表面疎水性をPEG/dextran系を用いる水性二相分配法, これに非イオン性界面活性剤Tritonを添加したTriton添加法, およびTriton/塩系の相分離を利用するTriton相分離法の3種類の二相分配法により体系的に評価した.タンパク質としては, 起源の異なる3種類のLipase, オリゴマーであるβ-Galactosidaseと, 酸の添加に対応して, Native状態, Molten-globuie状態, およびUnfold状態の3種類の高次構造を持つCytochrome cを用いた.タンパク質の疎水性は各々特徴的であり, 非常に個性的であるが, これらは少なくとも表面全体の疎水性と, 表面に局在する結合部位の疎水性の2種類に大別できる.前者は二相分配法により, 後者は, 他の2つの分配法によって定量できる.両者は, Molten-globule状態とUnfold状態のCytochrome cのように, 必ずしも同一の傾向を示さない.疎水クロマト法のような従来法では, 後者のみが測定される.これらタンパク質の疎水性の差異を利用してタンパク質の効果的な分離が可能である.
Super-resolution imaging with mid-IR photothermal microscopy on the single particle level (2015)

Photothermal microscopy has achieved single molecule sensitivity. However, the analytes are usually restricted to be natural absorbers in the visible light region. Mid-infrared (MIR) imaging, on the other hand, provides a wealth of information, but encounters difficulties of diffraction-limited spatial resolution and scarcity of ideal detectors. Here we present Mid-IR photothermal heterodyne imaging (MIR-PHI) microscopy as a high sensitivity, super-resolution mid-IR imaging technique. In MIR-PHI, a tunable Mid- IR pulsed laser at 150 kHz is used to excite a micron sized particle. Energy relaxation creates a temperature gradient around the particle, changing the refractive index of the surrounding solvent and creating a thermal lens. A collinear, counter propagating probe beam (a 532 nm CW laser) is modified by the thermal lens and generates a super-resolution photothermal image. We studied 1.1 &mu;m polystyrene beads at the single particle level using this technique. Various solvents with different heat capacities and refractive indices are tested for the best image contrast. The wide applicability and potentially high sensitivity of this technique make it promising for biological imaging and identification.