gebhart/patrepeval huggingface dataset:
Structure and Anticoagulant Activity of Sulfated Galactans (2000)

We have characterized the structure of a sulfated d-galactan from the red algae Botryocladia occidentalis. The following repeating structure (-4-alpha-d-Galp-1-->3-beta-d-Galp-1-->) was found for this polysaccharide, but with a variable sulfation pattern. Clearly one-third of the total alpha-units are 2,3-di-O-sulfated and another one-third are 2-O-sulfated. The algal sulfated d-galactan has a potent anticoagulant activity (similar potency as unfractionated heparin) due to enhanced inhibition of thrombin and factor Xa by antithrombin and/or heparin cofactor II. We also extended the experiments to several sulfated polysaccharides from marine invertebrates with simple structures, composed of a single repeating structure. A 2-O- or 3-O-sulfated l-galactan (as well as a 2-O-sulfated l-fucan) has a weak anticoagulant action when compared with the potent action of the algal sulfated d-galactan. Possibly, the addition of two sulfate esters to a single alpha-galactose residue has an "amplifying effect" on the anticoagulant action, which cannot be totally ascribed to the increased charge density of the polymer. These results indicate that the wide diversity of polysaccharides from marine alga and invertebrates is a useful tool to elucidate structure/anticoagulant activity relationships.
Multimodal Image Registration With Line Segments by Selective Search (2016)

This paper proposes a line segment-based image registration method. Edges are detected from images by a modified Canny operator, and line segments are then extracted from these edges. At registration, triplets (quaternions) of line segment correspondences are tentatively formed by applying the distance and orientation constraints, which determine an intermediate transformation. Those triplets (quaternions) of lines resulting in higher similarity metrics are preserved, and their intersections are refined by an iterative process or random sample consensus. The proposed method is tested on indoor and outdoor EO/IR image pairs, and the average registration error is calculated to be compared with existing algorithms. Experimental results show that the proposed registration method can robustly align EO/IR images containing line segments, providing more reliable and accurate registration results on multimodal images.
Role of Antibody to Native Type III Polysaccharide of Group B Streptococcus in Infant Infection (1981)

The role of maternally acquired antibody to native type III polysaccharide of group B Streptococcus as a determinant of susceptibility for infant systemic infection was investigated. Sera from 111 acutely ill infants with type III group B streptococcal bacteremia and/or meningitis and their mothers, and cord sera from 45 healthy neonates and their mothers who had type III group B streptococcal vaginal colonization at delivery were studied. Sera from each of 111 acutely ill infants contained very low levels of antibody (less than 1.7 microgram/ml, median 0.4 microgram/ml), and a significant correlation with maternal levels was tested for early onset infection (median 0.6 microgram/ml; 4 = .76; P less than .01). Women whose infants remained well had antibody levels greater than 2 microgram/ml in their sera (73%) more often than those whose infants developed symptomatic infection (17%) (P less than .001), and the median level in their sera (12.6 microgram/ml) was considerably higher. Study of sera obtained during convalescence from 86 surviving infants indicated a poor antibody response to infection. In contrast, high levels of antibody were detected in sera from each of five convalescent women with postpartum bacteremia. These data extend earlier observations suggesting the correlation between low levels of type-specific antibody in serum and risk for systemic infection with type III strains of group B streptococci.
Environmental and occupational risk factors for progressive supranuclear palsy: Case‐control study (2016)

We did not find evidence for a specific causative chemical exposure; higher number of years of drinking well water is a risk factor for PSP. This result remained significant after adjusting for income, smoking, education and occupational exposures. This is the first case-control study to demonstrate PSP is associated with environmental factors. © 2016 International Parkinson and Movement Disorder Society.
Rosuvastatin Decreases Caveolin-1 and Improves Nitric Oxide–Dependent Heart Rate and Blood Pressure Variability in Apolipoprotein E −/− Mice In Vivo (2003)

Rosuvastatin decreases caveolin-1 expression and promotes NOS function in apoE-/-, dyslipidemic mice in vivo, with concurrent improvements in BPV and HRV. This highlights the beneficial effects of rosuvastatin on cardiovascular function beyond those attributed to lipid lowering.
An instructive role for the interstitial matrix in tissue patterning: tissue segregation and intercellular invasion. (1990)

Intercellular invasion is the intrusion of the cells of one tissue into space occupied by a second tissue. The alternative situation to invasion, one characteristic of most coherent tissues, is segregation, with identifiable boundaries existing between contiguous tissues. The interfaces between mesenchymal and myocardial tissues in the developing avian heart show a profoundly different character in different regions of the heart: the interface between epicardial mesenchyme and heart wall myocardium is planar, without intermingling of the two cell types, whereas the interface between endocardial cushion mesenchyme and myocardium is diffuse, with extensive invasion of both tissue types across the border to produce intermingling of the two tissues. Thus, invasion and tissue segregation coexist in different regions of the mesenchyme-myocardium contact zone. Investigation of the involvement of the interstitial matrix in invasion and segregation has been conducted by maintaining the two tissues in mutual contact in organ culture. Investigation of the mechanisms by which the two cell types sort out in randomized chimeric tissue reaggregates has provided insight into the conditions for tissue segregation. We have modeled invasion in organ culture by fusing aggregates of myocardial cells with aggregates of cardiac mesenchymal cells. Cells of both tissues invaded the partner aggregate during a period of 1-3 d of coculture. Both invasion and segregation in the aggregates appear to depend on the presence or absence of a fibronectin-rich interstitial matrix elaborated by the cardiac mesenchyme. During sorting, the matrix appears selectively in regions occupied by the mesenchyme. Under conditions of culture that are nonpermissive for matrix deposition, sorting fails to occur. Stimulation of matrix deposition by addition of serum, transforming growth factor beta, or isolated matrix itself is accompanied by sorting out of the two tissues. Sorting out is blocked reversibly by inclusion of the fibronectin adhesion site peptide, GRGDSP. Invasion of fused aggregates is preceded by a redistribution of the fibronectin-containing matrix of the mesenchymal aggregate such that matrix-poor regions come to occupy the interface with the myocardial partner aggregate. The invasion that ensues involves mesenchymal cells emigrating from, and myocardial cells intruding into, matrix-poor regions of the mesenchymal aggregate.(ABSTRACT TRUNCATED AT 400 WORDS)
The Contribution of Mild and Moderate Preterm Birth to Infant Mortality (2000)

Mild- and moderate-preterm birth infants are at high RR for death during infancy and are responsible for an important fraction of infant deaths. JAMA. 2000;284:843-849
Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors. (2007)

We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
Perforated demineralized bone matrix: A new form of osteoinductive biomaterial (1986)

This study was undertaken to evaluate a new osteoinductive material--perforated decalcified bone matrix (PDBM). Subcutaneous implantation of PDBM induces multiple centers of endochondral osteogenesis with subsequent resorption of bone matrix and its replacement by new bone. PDBM should therefore prove useful as a research model to study osteoinduction and in the clinical situation in orthopedic and reconstructive surgery for the filling of bone defects and stimulation of fracture healing.
PTHrP Modulates Chondrocyte Differentiation through AP-1 and CREB Signaling (2001)

During the process of differentiation, chondrocytes integrate a complex array of signals from local or systemic factors like parathyroid hormone-related peptide (PTHrP), Indian hedgehog, bone morphogenetic proteins and transforming growth factor beta. While PTHrP is known to be a critical regulator of chondrocyte proliferation and differentiation, the signaling pathways through which this factor acts remain to be elucidated. Here we show that both cAMP response element-binding protein (CREB) and AP-1 activation are critical to PTHrP signaling in chondrocytes. PTHrP treatment leads to rapid CREB phosphorylation and activation, while CREB DNA binding activity is constitutive. In contrast, PTHrP induces AP-1 DNA binding activity through induction of c-Fos protein expression. PTHrP activates CRE and TRE reporter constructs primarily through PKA-mediated signaling events. Both signaling pathways were found to be important mediators of PTHrP effects on chondrocyte phenotype. Alone, PTHrP suppresses maturation and stimulates proliferation of the chondrocyte cultures. However, in the presence of dominant negative inhibitors of CREB and c-Fos, these PTHrP effects were suppressed, and chondrocyte maturation was accelerated. Moreover, in combination, the effects of dominant negative c-Fos and CREB are synergistic, suggesting interaction between these signaling pathways during chondrocyte differentiation.
In Vitro Biocontrol Activity of Methylobacterium Extorquens Against Fungal Pathogens (2009)

Accepted : February, 2009 Biological control is an environmentalfriendly strategy to reduce crop damage caused by plant pathogens. Increasing concerns about pesticide use by the general public and governmental agencies are severely limiting the availability and use of many important pesticides. Fungicides that have been used as standards in many disease control programme have been increasingly regulated. Research efforts with pathogens have indicated that most of the biocontrol organisms either did not control the pathogen or they did not perform as well as selected fungicides. Pink pigmented facultative methylotroph (PPFM) belonging to the genus Methylobacterium are a physiologically interesting group of bacteria that preferentially utilize methanol and other reduced one carbon compounds such as formate and formaldehyde as sole source of carbon and energy via serine pathway (Green, 1992).These are ubiquitous in nature and are thus found in a variety of habitats (Green and Bousifield, 1983), including soil, dust, fresh water, leaf surfaces and nodules. PPFMs isolated from the liverwort stimulated growth and development of the liverwort (Scapania nemorosa) and Streptocarpus prolixus (flowering plant) in tissue cultures, a positive commensal interaction was proposed (Corpe and Basile, 1982).The growth enhancing effects of PPFM on plants in a tissue culture system
New Approach for Integrity Bounds Computation Applied to Advanced Precise Positioning Applications (2015)

Precise Point Positioning (PPP) is a consolidated high precision positioning technique providing centimetre-level error. PPP processes dual-frequency pseudorange and carrier-phase measurements from a single user receiver, using detailed physical models and precise GNSS orbit & clock products calculated beforehand. PPP is different from other precise-positioning approaches like RTK in the sense that no reference stations are needed for obtaining the positioning solution. Another advantage of PPP is that since the GNSS orbit & clock products are global, the PPP solutions are global as well. PPP can be applied at post-processing level and also in real-time applications, provided that real-time input orbit and clock data are available. Our GNSS team has developed the algorithms and the data processing infrastructure needed for providing a real-time PPP service. All the required components, from the real time orbit and clock products generation, to the PPP filter implementation and the service management, are under our responsibility.

In some previous works, we laid down the foundations for an upper-level reliability concept for PPP, closely linked to the final user perspective, with the aim of providing a certain reliability bound for the required applications. The initial integrity/reliability concept for PPP has now been mathematically rigorously reformulated and is introduced in this paper. The current formulation incorporates all the initially identified relevant indicators, and is expressed as a generalization of the patented IBPL (Isotropy-Based Protection Level) technique to sequential estimation processes.

In order to illustrate the excellent bounding capabilities of the new approach for PPP integrity/reliability computation, three different scenarios have been considered: static, kinematic and convergence. Results about the performances of the provided bounds are going to be provided in each one of the considered cases, in terms of protection level magnitude, percentage of integrity failures and safety index versus number of integrity failures.
The Uranium Bomb, the Calutron, and the Space-Charge Problem (2005)

A participating scientist relates the story of a World War II project dedicated to electromagnetically separating uranium-235 from uranium-238.
Adenovirus‐mediated BMP2 expression in human bone marrow stromal cells (2001)

Recombinant adenoviral vectors have been shown to be potential new tools for a variety of musculoskeletal defects. Much emphasis in the field of orthopedic research has been placed on developing systems for the production of bone. This study aims to determine the necessary conditions for sustained production of high levels of active bone morphogenetic protein 2 (BMP2) using a recombinant adenovirus type 5 (Ad5BMP2) capable of eliciting BMP2 synthesis upon infection and to evaluate the consequences for osteoprogenitor cells. The results indicate that high levels (144 ng/ml) of BMP2 can be produced in non-osteoprogenitor cells (A549 cell line) by this method and the resultant protein appears to be three times more biologically active than the recombinant protein. Surprisingly, similar levels of BMP2 expression could not be achieved after transduction with Ad5BMP2 of either human bone marrow stromal cells or the mouse bone marrow stromal cell line W20-17. However, human bone marrow stromal cells cultured with 1 microM dexamethasone for four days, or further stimulated to become osteoblast-like cells with 50 microg/ml ascorbic acid, produced high levels of BMP2 upon Ad5BMP2 infection as compared to the undifferentiated cells. The increased production of BMP2 in adenovirus transduced cells following exposure to 1 microM dexamethasone was reduced if the cells were not given 50 microg/ml ascorbic acid. When bone marrow stromal cells were allowed to become confluent in culture prior to differentiation, BMP2 production in response to Ad5BMP2 infection was lost entirely. Furthermore, the increase in BMP2 synthesis seen during differentiation was greatly decreased when Ad5BMP2 was administered prior to dexamethasone treatment. In short, the efficiency of adenovirus mediated expression of BMP2 in bone marrow stromal cells appears to be dependent on the differentiation state of these cells.
Monoclonal Antibodies to Human 54,000 Molecular Weight Plasminogen Activator Inhibitor from Fibrosarcoma Cells - Inhibitor Neutralization and One-Step Affinity Purification (1986)

Mouse monoclonal antibodies were derived against a plasminogen activator inhibitor with a mol. wt. of approximately 54,000 (54 K) from the human fibrosarcoma cell line HT-1080. Screening for hybrids producing antibodies directed against the inhibitor was performed with enzyme-linked immunosorbent assay and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting. Four clones of hybridomas producing IgG1 antibodies were further characterized. The inhibitor was purified approximately 50-fold to homogeneity from conditioned cell culture fluid with a yield of approximately 85% by a one-step procedure using Sepharose-conjugated monoclonal antibody. In the 125I-fibrin plate assay one of the antibodies neutralized the effect of the inhibitor on urokinase-type plasminogen activator. Two of the antibodies bound complexes between urokinase-type plasminogen activator and inhibitor while the remaining two antibodies did not. The antibodies could be used for immunocytochemical localization of the inhibitor in HT-1080 cells. All four antibodies cross-reacted with a plasminogen activator inhibitor derived from cultured human umbilical cord endothelial cells.
Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10 (2008)

We previously reported Frizzled homolog 10 (FZD10), a member of the Frizzled family, to be a promising therapeutic target for synovial sarcomas. In this report, we established a murine monoclonal antibody (MAb), namely, MAb 92-13 that had specific binding activity against native FZD10 product expressed in synovial sarcoma cell lines. Subsequent immunohistochemical analyses with the MAb 92-13 confirmed an absence or hardly detectable level of FZD10 protein in any normal human organs. We confirmed the specific binding activity of this MAb in vivo after injection of fluorescent-labeled MAb i.p. or i.v. into the mice carrying synovial sarcoma xenografts by the use of the in vivo fluorescent imaging system as well as radioisotopes. Moreover, MAb 92-13 was effectively internalized into the synovial sarcoma cells after its binding to FZD10 on the cell surface. A single i.v. injection of the Yttrium-90 ((90)Y)-MAb 92-13 drastically suppressed tumor growth of synovial sarcoma in mice without any severe toxicity. Median time to tumor progression was 58 days for mice treated with (90)Y-MAb 92-13 and 9 days for mice treated with non-labeled antibody control or untreated mice (difference = 49 days; P = 7 x 10(-5)). This result indicates that MAb 92-13 could be utilized as the novel treatment modality for synovial sarcoma and other FZD10-positive tumors.
The Nijmegen Modification of the Bethesda Assay for Factor VIII:C Inhibitors: Improved Specificity and Reliability (1995)

Antibodies against factor VIII coagulant activity can appear in haemophiliacs who are treated with factor VIII preparations but also spontaneously in non-haemophiliacs. The Bethesda assay is the most commonly used method to detect these antibodies, but it lacks specificity especially in the lower range resulting in unreliable data. Two modifications are proposed and tested to resolve the imperfections: 1. Buffering the normal plasma used in the assay- and control mixture with 0.1 M imidazole to pH 7.4. 2. Replacing the imidazole buffer in the control mixture by immunodepleted factor VIII deficient plasma. These modifications allow better discrimination between positive and negative samples and improve reliability.
Enhanced MET Translation and Signaling Sustains K-Ras–Driven Proliferation under Anchorage-Independent Growth Conditions (2015)

Oncogenic K-Ras mutation occurs frequently in several types of cancers, including pancreatic and lung cancers. Tumors with K-Ras mutation are resistant to chemotherapeutic drugs as well as molecular targeting agents. Although numerous approaches are ongoing to find effective ways to treat these tumors, there are still no effective therapies for K-Ras mutant cancer patients. Here we report that K-Ras mutant cancers are more dependent on K-Ras in anchorage-independent culture conditions than in monolayer culture conditions. In seeking to determine mechanisms that contribute to the K-Ras dependency in anchorage-independent culture conditions, we discovered the involvement of Met in K-Ras-dependent, anchorage-independent cell growth. The Met signaling pathway is enhanced and plays an indispensable role in anchorage-independent growth even in cells in which Met is not amplified. Indeed, Met expression is elevated under anchorage-independent growth conditions and is regulated by K-Ras in a MAPK/ERK kinase (MEK)-dependent manner. Remarkably, in spite of a global downregulation of mRNA translation during anchorage-independent growth, we find that Met mRNA translation is specifically enhanced under these conditions. Importantly, ectopic expression of an active Met mutant rescues K-Ras ablation-derived growth suppression, indicating that K-Ras-mediated Met expression drives "K-Ras addiction" in anchorage-independent conditions. Our results indicate that enhanced Met expression and signaling is essential for anchorage-independent growth of K-Ras mutant cancer cells and suggests that pharmacological inhibitors of Met could be effective for K-Ras mutant tumor patients.
The intensification of rapid reactions in multiphase systems using slug flow in capillaries (2001)

A multiphase microreactor based upon the use of slug flow through a narrow channel has been developed. The internal circulation, which is stimulated within the slugs by their passage along the channel, is responsible for a large enhancement in the interfacial mass transfer and the reaction rate. Mass transfer performance data has been obtained for a glass chip-based reactor in a 380 microm wide channel by monitoring the extraction of acetic acid from kerosene slugs as they moved along the reactor channel. Finally, the data was compared with that provided from other inter-phase contacting techniques.
Early lymphocyte recovery post‐autologous haematopoietic stem cell transplantation is associated with better survival in Hodgkin's disease (2002)

A retrospective study of 82 patients was conducted to determine the relationship of absolute lymphocyte count (ALC) recovery with clinical outcome after autologous stem cell transplantation (ASCT) in Hodgkin's disease (HD). The median overall (OS) and progression-free survival (PFS) times from the day of transplantation were significantly better for the 41 patients with ALC > or = 0.5 x 10(9) cells/l compared with the 41 patients with ALC < 0.5 x 10(9) cells/l ("not yet reached" versus 42 months, P < 0.0001; 57 versus 15 months, P < 0.002 respectively). Thus, ALC recovery on day 15 post ASCT in HD is associated with better survival and requires further study.
Epstein-barr virus and human malignancies (1974)

EBV has remained the foremost contender for “the first human cancer virus.” It is the cause of infectious mononucleosis, which has been considered a self-limited leukemia. Self-limitation could fail to materialize on rare occasions and, indeed, evidence has been mounting for an etiologic role of EBV in Burkitt's lymphoma (BL) of African children, for which an infectious cause has been postulated on the basis of epidemiologic observations. EBV might be causally related also to nasopharyngeal carcinoma (NPC). EBV fingerprints are found in nearly every BL or NPC biopsy; EBV transforms lymphocytes in vitro into permanently growing lymphoblasts resembling BL cells; EBV induces lympho-proliferative tumors in certain nonhuman primates; and all African BL and Chinese or other NPC patients have antibodies to various EBV-determined antigens, often at high titers, whereas controls have lower titers of some of the antibodies and lack others. Changes in the spectra and titers of antibodies reflect the clinical status and prognosis in BL and the stage of the disease and success of therapy in NPC. These facts oppose a mere passenger role of EBV, but other rare, as yet unknown factors must undoubtedly contribute to expression of its oncogenic potential.