gebhart/patrepeval huggingface dataset:
T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell–cell contacts (2001)

The product of the Lymphocyte Activation Gene-3 (LAG-3, CD223) is a high affinity MHC class II ligand expressed by activated CD4(+) and CD8(+) T cells, which can associate with the T cell receptor (TCR) and downregulate TCR signalling in vitro. We have also reported that a soluble mLAG-3Ig fusion protein works as a vaccine adjuvant in vivo in mice, enhancing Th1 and CD8 T cell responses. Here, we report that LAG-3 expression was found, using fluorescent activated cell sorting (FACS) analysis, on 11-48% of human tumour-infiltrating lymphocytes (TILs) isolated from eight freshly dissociated renal cell carcinomas (RCCs), and was restricted mostly to CD8(+) cells. Immunohistochemical analysis confirmed LAG-3 expression by TILs in 9/11 RCCs, as well as in tumours of different origins, such as melanomas (3/5) and lymphomas (7/7). Since not only antigen presenting cells (APCs), but also TILs themselves strongly express major histocompatibility complex (MHC) class II, we firstly investigated whether LAG-3/MHC class II T-T cell contacts might influence tumour cell recognition. However, cytotoxicity inhibition was not observed in two RCC-specific CD8(+) T cell clones in the presence of the LAG-3-specific MAb, and there was also no observed difference in the recognition of LAG-3-transfected or wild-type RCC by these cytotoxic T lymphocytes (CTLs). In contrast, MHC class II engagement by LAG-3Ig was found to enhance the capacity of immature dendritic cells to stimulate naive T cell proliferation and IL-12-dependent IFN-gamma production by T cells in vitro. These results therefore provide support for a role for TIL-expressed LAG-3 in the engagement of class II molecules on APCs, thereby contributing to APC activation and Th1/Tc1 commitment, without downregulating cytotoxicity.
Supermode Si/III-V hybrid lasers, optical amplifiers and modulators: A proposal and analysis (2007)

We describe a hybrid laser structure which consists of an amplifying III-V waveguide proximity-coupled to a passive Si waveguide. By operating near the synchronism point (where the phase velocities of the individual waveguides are equal), we can cause the optical power to be confined to any of the two waveguides. This is accomplished by control of waveguides' geometry. In the portion of the supermode resonator where amplification takes place, the mode is confined nearly completely to III-V guide thus realizing a near maximal gain. Near the output facet, the mode power is confined to the Si waveguide thus optimizing the output coupling. This is to be contrasted with approaches which depend on evanescent field penetration into the III-V medium to obtain gain.
rAAV/Her-2/neu Loading of Dendritic Cells for a Potent Cellular-Mediated MHC Class I Restricted Immune Response Against Ovarian Cancer (2008)

Recent studies demonstrate that recombinant adeno-associated virus (rAAV)-based antigen loading of dendritic cells (DCs) generates, in vitro, significant and rapid cytotoxic T-lymphocyte (CTL) responses against viral antigens. We used the rAAV system to induce specific CTLs against tumor antigens for the development of ovarian cancer (OC) gene therapy. As an extension of the versatility of the rAAV system, we incorporated a self-antigen, Her-2/neu, which is expressed in many cancers, including breast and ovarian. We analyzed two different vectors containing a short (157-612) and long domain (1-1197). Our rAAV vector induced strong stimulation of CTLs directed against the self tumor antigen, Her-2/neu. We then investigated the efficiency of the CTLs in killing Her-2/neu-targeted cells. A significant MHC class I-restricted, anti-Her-2/neu-specific CTL killing was demonstrated against Her-2/neu-positive OC cells after one in vitro stimulation. In summary, single peripheral blood mononuclear cell (PBMC) stimulation with rAAV/157-612- or rAAV/1-1197-pulsed DCs induces strong antigen-specific CTL generation. The CTLs were capable of lysing low doses of peptides pulsed into target cells or OC Her-2/neu(+) tumors. These data suggest that AAV-based antigen loading of DCs is highly effective for generating human CTL responses against OC antigens.
AutoAugment: Learning Augmentation Strategies From Data (2019)

Data augmentation is an effective technique for improving the accuracy of modern image classifiers. However, current data augmentation implementations are manually designed. In this paper, we describe a simple procedure called AutoAugment to automatically search for improved data augmentation policies. In our implementation, we have designed a search space where a policy consists of many sub-policies, one of which is randomly chosen for each image in each mini-batch. A sub-policy consists of two operations, each operation being an image processing function such as translation, rotation, or shearing, and the probabilities and magnitudes with which the functions are applied. We use a search algorithm to find the best policy such that the neural network yields the highest validation accuracy on a target dataset. Our method achieves state-of-the-art accuracy on CIFAR-10, CIFAR-100, SVHN, and ImageNet (without additional data). On ImageNet, we attain a Top-1 accuracy of 83.5% which is 0.4% better than the previous record of 83.1%. On CIFAR-10, we achieve an error rate of 1.5%, which is 0.6% better than the previous state-of-the-art. Augmentation policies we find are transferable between datasets. The policy learned on ImageNet transfers well to achieve significant improvements on other datasets, such as Oxford Flowers, Caltech-101, Oxford-IIT Pets, FGVC Aircraft, and Stanford Cars.
Calcium‐deficient hydroxyapatite‐PLGA composites: Mechanical and microstructural investigation (2000)

The microstructural and mechanical properties of composites composed of calcium deficient hydroxyapatite (CDHAp) and poly(lactide-co-glycolide) (PLGA) have been investigated. The composites were formed by hydrolysis of α-tricalcium phosphate (α-TCP) to CDHAp in pressed precomposite compacts of α-TCP-PLGA-NaCl. The differences in hydrolysis of α-TCP-PLGA-NaCl for two compositions of 80:10:10 wt % and 60:20:20 wt %. were monitored by isothermal calorimetry and X-ray diffraction. The microstructural evolution and variance in final composite microstructure after hydrolysis at 37°C, 45°C, and 56°C were examined by scanning electron microscopy. HAp–PLGA composite formed from the α-TCP-PLGA-NaCl (80:10:10) precomposites at 37°C developed a tensile strength of 13.3 ± 0.9 MPa, a flexural strength of 24.8 ± 1.7 MPa, and Young's modulus of 2.8 ± 0.3 GPa. These values were 12.00 ± 0.2 MPa, 36.1 ± 2.1 MPa, and 5.5 ± 0.8 GPa for the precomposite composition 60:20:20. All these mechanical properties showed a variation with hydrolysis temperature and composition. The differences in mechanical properties were related to the final microstructures of the composites, which are governed by the morphological changes in the polymer structure at its glass transition temperature and the extent of cement-type formation of CDHAp by hydrolysis of α-TCP. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 51, 726–734, 2000.
Deletion of the long arm of chromosome 20 in a patient with chronic neutrophilic leukemia: Cytogenetic findings in chronic neutrophilic leukemia (1997)

We encountered a 67-year-old female with chronic neutrophilic leukemia (CNL). Cytogenetic study showed she had a deletion in the long arm of chromosome 20. This finding indicates that CNL, in this case, is a clonal disorder. Most CNL patients have normal karyotypes, and only four patients with cytogenetic abnormalities, including two cases who received chemotherapy before the cytogenetic abnormality was detected, have been reported. Four of those cases, including our case, had abnormalities in the long arm of chromosome 20. This locus may be associated with the development of CNL. To our knowledge, this is the first case with CNL who showed deletion of the long arm of chromosome 20 before treatment was started. Am. J. Hematol. 54:72–75, 1997 © 1997 Wiley-Liss, Inc.
BIM Case Studies (2018)

This chapter presents case studies of projects in which Building Information Modeling (BIM) played a significant role. They represent the experiences of owners, architects, engineers, contractors, fabricators, and even construction crews and a facility maintenance team—all pioneers in the application of BIM. Each case study demonstrates a diverse set of benefits to various organizations, resulting from the implementation of BIM tools and processes. Each case study presents the salient aspects of the BIM process and focuses on the ways each team used the available tools to maximum benefit. The chapter highlights the many lessons that these teams learned as they encountered challenges in implementing the new technologies and processes. The case studies cover the use of BIM across all phases of the facility delivery process by a wide range of project participants.
Synthesis and biological activity of tripeptidyl polyoxins as antifungal agents (1983)

Three tripeptidyl polyoxins were synthesized and found to inhibit Candida albicans. Compared with the naturally occurring polyoxin D, the three synthetic polyoxins had little effect on chitin synthetase when assayed with a C. albicans membrane preparation. However, all the compounds inhibited growth, affected cell morphology in a manner similar to that of polyoxin D, and were hydrolyzed by cell extracts of C. albicans. Hydrolysis did not occur extracellularly, and at least one of the synthetic polyoxins, leucyl-norleucyl-uracil polyoxin C, inhibited peptide uptake, suggesting entrance into the cell via the peptide transport system. Thus, the intact tripeptidyl polyoxins are inactive prodrugs that are converted to active moieties by cellular enzymes.
Association between Prior Cytomegalovirus Infection and the Risk of Restenosis after Coronary Atherectomy (1996)

Prior infection with CMV is strong independent risk factor for restenosis after coronary atherectomy. If confirmed, these findings may help identify patients at risk for restenosis.
Surprise! Higher Dividends = Higher Earnings Growth (2003)

We investigate whether dividend policy, as observed in the payout ratio of the U.S. equity market portfolio, forecasts future aggregate earnings growth. The historical evidence strongly suggests that expected future earnings growth is fastest when current payout ratios are high and slowest when payout ratios are low. This relationship is not subsumed by other factors, such as simple mean reversion in earnings. Our evidence thus contradicts the views of many who believe that substantial reinvestment of retained earnings will fuel faster future earnings growth. Rather, it is consistent with anecdotal tales about managers signaling their earnings expectations through dividends or engaging, at times, in inefficient empire building. Our findings offer a challenge to market observers who see the low dividend payouts of recent times as a sign of strong future earnings to come.
Concomitant loss of chromosome 3 and whole arm losses and gains of chromosome 1, 6, or 8 in metastasizing primary uveal melanoma. (2001)

Loss of chromosome 3, loss of 6q, and gain of 8q were significantly associated with poor overall survival. In addition, losses of 1p were only found in primary uveal melanomas that had metastasized and in metastases, which suggests that this region may harbor a tumor suppressor gene important in the tumor progression. Finally, loss of chromosome 3 may be associated with isochromosome formation of 1q, 6p, 8q, 16p, 20q, and 22q.
Single cell epitaxy by acoustic picolitre droplets (2007)

The capability to encapsulate single to few cells with micrometre precision, high viability, and controlled directionality via a nozzleless ejection technology using a gentle acoustic field would have great impact on tissue engineering, high throughput screening, and clinical diagnostics. We demonstrate encapsulation of single cells (or a few cells) ejected from an open pool in acoustic picolitre droplets. We have developed this technology for the specific purpose of printing cells in various biological fluids, including PBS and agarose hydrogels used in tissue engineering. We ejected various cell types, including mouse embryonic stem cells, fibroblasts, AML-12 hepatocytes, human Raji cells, and HL-1 cardiomyocytes encapsulated in acoustic picolitre droplets of around 37 µm in diameter at rates varying from 1 to 10 000 droplets per second. At such high throughput levels, we demonstrated cell viabilities of over 89.8% across various cell types. Moreover, this ejection method is readily adaptable to other biological applications, such as extracting data from single cells and generating large cell populations from single cells. The technique described in the current study may also be applied to investigate stem cell differentiation at the single cell level, to direct tissue printing, and to isolating pure RNA or DNA from a single cell at the picolitre level. Overall, the techniques described have the potential for widespread impact on many high-throughput testing applications in the biological and health sciences.
Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition (2004)

We recently used a positional cloning approach to identify a nonconservative lysine to alanine substitution (K232A) in the bovine DGAT1 gene that was proposed to be the causative quantitative trait nucleotide underlying a quantitative trait locus (QTL) affecting milk fat composition, previously mapped to the centromeric end of bovine chromosome 14. We herein generate genetic and functional data that confirm the causality of the DGAT1 K232A mutation. We have constructed a high-density single-nucleotide polymorphism map of the 3.8-centimorgan BULGE30-BULGE9 interval containing the QTL and show that the association with milk fat percentage maximizes at the DGAT1 gene. We provide evidence that the K allele has undergone a selective sweep. By using a baculovirus expression system, we have expressed both DGAT1 alleles in Sf9 cells and show that the K allele, causing an increase in milk fat percentage in the live animal, is characterized by a higher Vmax in producing triglycerides than the A allele.
Nestin in gastrointestinal and other cancers: Effects on cells and tumor angiogenesis (2011)

Nestin is a class VI intermediate filament protein that was originally described as a neuronal stem cell marker during central nervous system (CNS) development, and is currently widely used in that capacity. Nestin is also expressed in non-neuronal immature or progenitor cells in normal tissues. Under pathological conditions, nestin is expressed in repair processes in the CNS, muscle, liver, and infarcted myocardium. Furthermore, increased nestin expression has been reported in various tumor cells, including CNS tumors, gastrointestinal stromal tumors, pancreatic cancer, prostate cancer, breast cancer, malignant melanoma, dermatofibrosarcoma protuberances, and thyroid tumors. Nestin is reported to correlate with aggressive growth, metastasis, and poor prognosis in some tumors; however, the roles of nestin in cancer cells have not been well characterized. Furthermore, nestin is more specifically expressed in proliferating small-sized tumor vessels in glioblastoma and gastric, colorectal, and prostate cancers than are other tumor vessel markers. These findings indicate that nestin may be a marker for newly synthesized tumor vessels and a therapeutic target for tumor angiogenesis. It has received a lot of attention recently as a cancer stem cell marker in various cancer cells including brain tumors, malignant rhabdoid tumors, and uterine, cervical, prostate, bladder, head and neck, ovarian, testicular, and pancreatic cancers. The purpose of this review is to clarify the roles of nestin in cancer cells and in tumor angiogenesis, and to examine the association between nestin and cancer stem cells. Nestin has the potential to serve as a molecular target for cancers with nestin-positive cancer cells and nestin-positive tumor vasculature.
Relation of nicotine yield of cigarettes to blood nicotine concentrations in smokers. (1980)

Blood nicotine and carboxyhaemoglobin (COHb) concentrations were studied in 330 smokers (206 women and 124 men). Blood nicotine concentrations in individual smokers varied from 25 to 444 nmol/l (4 to 72 ng/ml). The average concentration, 203 nmol/l (33 ng/ml), was the same in the men and the women, although cigarette consumption was higher in the men. Despite large differences in nicotine yield, there was no relation between blood nicotine concentration and the type of cigarette smoked: smokers of plain, untipped cigarettes (1.9 mg nicotine), cigarettes with unventilated filters (1.3 mg nicotine), and cigarettes with ventilated filters (0.8 mg nicotine) had similar blood nicotine concentrations. Cigarette consumption was also similar in these three groups. The correlation between blood nicotine concentration and nicotine yield of cigarette, though significant, was low (0.21, p < 0.001), showing that the nicotine yield of the cigarettes accounted for only 4.4% of the variation in blood nicotine concentrations. Similarly, the low correlation of 0.30 between COHb concentration and cigarette consumption suggests that cigarette consumption accounted for only 9% of the variation in the amount of smoke taken into the smokers' lungs. These results suggest that the assumed health advantage of switching to lower-tar and lower-nicotine cigarettes may be largely offset by the tendency of smokers to compensate by increasing inhalation. The findings of epidemiological studies showing lower risks with filter-tipped cigarettes may be attributable to other factors such as biases in the samples and changes in the quality and carcinogenicity of tobacco tar, rather than to reduced tar intake.
Design of capacity-approaching irregular low-density parity-check codes (2001)

We design low-density parity-check (LDPC) codes that perform at rates extremely close to the Shannon capacity. The codes are built from highly irregular bipartite graphs with carefully chosen degree patterns on both sides. Our theoretical analysis of the codes is based on the work of Richardson and Urbanke (see ibid., vol.47, no.2, p.599-618, 2000). Assuming that the underlying communication channel is symmetric, we prove that the probability densities at the message nodes of the graph possess a certain symmetry. Using this symmetry property we then show that, under the assumption of no cycles, the message densities always converge as the number of iterations tends to infinity. Furthermore, we prove a stability condition which implies an upper bound on the fraction of errors that a belief-propagation decoder can correct when applied to a code induced from a bipartite graph with a given degree distribution. Our codes are found by optimizing the degree structure of the underlying graphs. We develop several strategies to perform this optimization. We also present some simulation results for the codes found which show that the performance of the codes is very close to the asymptotic theoretical bounds.
Contraception: Your Questions Answered (1985)

1. Introduction: the population explosion, sexual and contraceptive history-taking and counselling - the importance of fertility control. 2. Aspects of human fertility and fertility awareness: natural birth control. 3. Male methods of contraception. 4. Vaginal methods of contraception. 5. The combined oral contraceptive - selection and eligibility. 6. The combined oral contraceptive - follow-up arrangements and new routes of administration. 7. The progestogen-only pill. 8. Injectables and implants. 9. Intrauterine devices and systems. 10. Emergency (postcoital) contraception. 11. Contraception for the young, the not quite so young - and in future. Further reading. Websites. Appendices.
Review of Stereo Vision Algorithms: From Software to Hardware (2008)

Stereo vision, resulting in the knowledge of deep information in a scene, is of great importance in the field of machine vision, robotics and image analysis. In this article, an explicit analysis of the existing stereo matching methods, up to date, is presented. The presented algorithms are discussed in terms of speed, accuracy, coverage, time consumption, and disparity range. Towards the direction of real-time operation, the development of stereo matching algorithms, suitable for efficient hardware implementation is highly desirable. Implementations of stereo matching algorithms in hardware for real-time applications are also discussed in details.
Broad spectrum effects of secondary metabolites from the red algadelisea pulchrain antifouling assays (1995)

In this study the antifouling activity was investigated of a series of chemically related, halogenated furanones&#13;\nisolated from Delisea pulchra (Greville) Montagne, a red alga which is rarely fouled in the field. The&#13;\nmetabolites were tested in laboratory assays against representatives of the three major groups of fouling&#13;\norganisms, the barnacle Baianus amphitrite amphitirite Darwin, the macroalga Ulva lactuca Linnaeus and a&#13;\nmarine bacterium (strain SW 8). Settlement of barnacle cyprid larvae was strongly inhibited, with an EC50 of&#13;\n&lt; 25 ng·mr1 (25 ppb) for some compounds. The settlement and growth of algal gametes was also strongly&#13;\ninhibited, in some cases at concentrations as low as 25 ng·cm-2&#13;\n• Growth of the marine bacterium SW8 was&#13;\ninhibited more strongly than by the common antibiotic gentamicin. Overall, activity of the D. pulchra&#13;\nmetabolites was comparable to that of the heavy metals and biocides currently used in antifouling paints.&#13;\nHowever, no single compound was most active in all tests and some metabolites effective against one organism&#13;\nshowed Iittle or no activity a·gainst the others. The high but variable level of activity of the D. pulchra&#13;\nmetabolites, coupled with their small size, relative stability, and ability tobe synthesized suggest their potential&#13;\nuse as active ingredients in antifouling coatings.
Tumour Cell Generation of Inducible Regulatory T-Cells in Multiple Myeloma Is Contact-Dependent and Antigen-Presenting Cell-Independent (2012)

Regulatory T-cells (T(Reg) cells) are increased in patients with multiple myeloma (MM). We investigated whether MM cells could generate and/or expand T(Reg) cells as a method of immuno-surveillance avoidance. In an in vitro model, CD4(+)CD25(-)FoxP3(-) T-cells co-cultured with malignant plasma cells (primary MM cells and cell lines) induced a significant generation of CD4(+)CD25(+)FoxP3(+) inducible T(Reg) cells (tT(Reg) cells; p<0.0001), in a contact-dependent manner. tT(Reg) cells were polyclonal, demonstrated a suppressive phenotype and phenotypically, demonstrated increased FoxP3 (p = 0.0001), increased GITR (p<0.0001), increased PD1 (p = 0.003) and decreased CD62L (p = 0.007) expression compared with naturally occurring T(Reg) cells. FACS-sorted tT(Reg) cells differentiated into FoxP(+)IL-17(+) and FoxP3(-)IL-17(+) CD4(+) cells upon TCR-mediated stimulation. Blocking experiments with anti-ICOS-L MoAb resulted in a significant inhibition of tT(Reg) cell generation whereas both IL-10 & TGFβ blockade did not. MM tumour cells can directly generate functional T(Reg) cells in a contact-dependent manner, mediated by ICOS/ICOS-L. These features suggest that tumour generation of T(Reg) cells may contribute to evasion of immune surveillance by the host.