gebhart/patrepeval huggingface dataset:
Degradation and Recycling of the Substrate-binding Subunit of Type II Iodothyronine 5′-Deiodinase in Astrocytes (1996)

Thyroxine dynamically regulates levels of type II iodothyronine 5'-deiodinase (5'D-II) by modulating enzyme inactivation and targeting the enzyme to different pathways of internalization. 5'D-II is an approximately 200-kDa multimeric protein containing a 29-kDa substrate-binding subunit (p29) and an unknown number of other subunits. In the absence of thyroxine (T4), p29 is slowly endocytosed and transported to the lysosomes. T4 treatment rapidly activates an actin-mediated endocytotic pathway and targets the enzyme to the endosomes. In this study, we have characterized the influence of T4 on the intracellular trafficking of 5'D-II. We show that T4 accelerates the rate of 5'D-II inactivation by translocating the enzyme to the interior of the cell and by sequestering p29 in the endosomal pool without accelerating the rate of degradation of p29. This dichotomy between the rapid inactivation of catalytic activity and the much slower degradation of p29 is consistent with the reuse of p29 in the production of 5'D-II activity. Immunocytochemical analysis with a specific anti-p29 IgG shows that pulse affinity-labeled p29 reappears on the plasma membrane approximately 2 h after enzyme internalization in the presence of T4, indicating that p29 is recycled. Despite the ability of p29 to be recycled in the T4-treated cell, 5'D-II catalytic activity requires ongoing protein synthesis, presumably of another enzyme component(s) or an accessory enzyme-related protein. In the absence of T4, enzyme inactivation and p29 degradation are temporally linked, and pulse affinity-labeled p29 is internalized and sequestered in discrete intracellular pools. These data suggest that T4 regulates fundamental processes involved with the turnover of integral membrane proteins and participates in regulating the inter-relationships between the degradation, recycling, and synthetic pathways.
A surface plasmon resonance study of albumin adsorption to PEO-PPO-PEO triblock copolymers (1998)

Pluronic surfactants, PEO-PPO-PEO triblock copolymers, have been investigated widely due to their protein-resistant properties in applications as coatings for implants and in controlled drug release systems. We have studied a wide range of these copolymers, varying in both PEO and PPO block size, by adsorbing them to a polystyrene surface and investigating their subsequent resistance to human serum albumin adsorption. This investigation has been carried out in real time, using surface plasmon resonance, with the surfaces subsequently visualized by atomic force microscopy. This approach has allowed determination of the effect of the lengths of the PEO and PPO polymer chains on protein resistivity. For low-molecular-weight Pluronics a significant, yet not complete, reduction in albumin adsorption has been observed whereas higher molecular weight Pluronics appear to completely inhibit adsorption within the time frame of this experiment. An increase in the PPO block size of the copolymer also appears to increase its protein resistance. This work further confirms that the binding strength of the anchoring block to the hydrophobic surface, rather than the length of the protruding hydrophilic PEO chains, determines a copolymer's protein resistance capability.
The Protease Inhibitor Bikunin, a Novel Anti-Metastatic Agent (2003)

Bikunin is a Kunitz-type protease inhibitor predominantly found in human amniotic fluid. In cancers, administration of bikunin may block tumor cell invasion by a direct inhibition of tumor cell-associated plasmin activity as well as by inhibiting urokinase-type plasminogen activator (uPA) expression at the gene and protein levels, possibly through suppression of CD44 dimerization and/or the MAP kinase signaling cascade. Treatment of cancer patients with bikunin may be beneficial in the adjuvant setting to delay the onset of metastasis development and/or in combination with cytotoxic agents to improve treatment efficacy in patients with advanced ovarian cancer.
Improvement in light-output efficiency of InGaN/GaN multiple-quantum well light-emitting diodes by current blocking layer (2002)

The fabrication and characterization of an InGaN/GaN multiple-quantum well (MQW) light-emitting diode (LED) with a SiO2 current blocking layer inserted beneath the p-pad electrode is described. The light-output power and external quantum efficiency for the InGaN/GaN MQW LED chip with a current blocking layer were significantly increased compared to those for the conventional InGaN/GaN MQW LED chip. The increase in the light-output power can be attributed to the injection of additional current into the light-emitting quantum well layer of the LED by the SiO2 current blocking layer and a reduction in parasitic optical absorption in the p-pad electrode.
Design and verification of energy detection in wireless body area networks (2009)

The amplitude response characteristics of body channel in wireless body area networks versus body channel length and signal frequencies are analyzed in this paper.Then the energy detection formula based on the above body channel also are derived to formulate the energy detection algorithm in body area networks.In the end,this paper comes to the following conclusion: the detection possibility based on the derived energy detection algorithm versus channel length and signal frequencies is given in the condition of long enough detection time while signal energy changes from-128dBm to-110dBm.
Enhancement of in vitro and in vivo antigen-specific antibody responses by interleukin 11. (1992)

The availability of large quantities of highly purified recombinant interleukin 11 (rhuIL-11) has allowed us to investigate the effects of rhuIL-11 on sheep red blood cell (SRBC)-specific antibody responses in the murine system. The results showed that rhuIL-11 was effective in enhancing the generation of mouse spleen SRBC-specific plaque-forming cells (PFC) in the in vitro cell culture system in a dose-dependent manner. These effects of rhuIL-11 were abrogated completely by the addition of anti-rhuIL-11 antibody, but not by the addition of preimmunized rabbit serum. Cell-depletion studies revealed that L3T4 (CD4)+ T cells, but not Lyt-2 (CD8)+ T cells, are required in the rhuIL-11-stimulated augmentation of SRBC-specific antibody responses. The effects of rhuIL-11 on the SRBC-specific antibody responses in vivo were also examined. RhuIL-11 administration to normal C3H/HeJ mice resulted in a dose-dependent increase in the number of spleen SRBC-specific PFC as well as serum SRBC-specific antibody titer in both the primary and secondary immune responses. In mice immunosuppressed by cyclophosphamide treatment, rhuIL-11 administration significantly augmented the number of spleen SRBC-specific PFC as well as serum SRBC-specific antibody titer when compared with the cyclophosphamide-treated mice without IL-11 treatment. These results demonstrated that IL-11 is a novel cytokine involved in modulating antigen-specific antibody responses in vitro as well as in vivo.
The positional distribution of fatty acids in Escherichia coli phospholipids is not regulated by sn-glycerol 3-phosphate levels (1980)

Large changes in the intracellular concentration of sn-glycerol 3-phosphate had no effect on the acyl chain distribution of the phospholipids of Escherichia coli. This result directly contradicts the prediction by other workers based on in vitro experiments.
Functional group diversity by ruthenium-catalyzed olefin cross-metathesis (2002)

Abstract Ruthenium-catalyzed olefin cross-metathesis tolerates a wide range of functional groups, including phosphine-boranes, sulfides, amines, phenols, and oxazolines. The high functional group tolerance allows for the use of an olefin as a linchpin for the synthesis of a variety of bi-, tri-, and tetradentate chiral ligands with a high degree of functional group diversity.
N-terminal Glutamate to Pyroglutamate Conversion in Vivo for Human IgG2 Antibodies (2011)

Therapeutic proteins contain a large number of post-translational modifications, some of which could potentially impact their safety or efficacy. In one of these changes, pyroglutamate can form on the N terminus of the polypeptide chain. Both glutamine and glutamate at the N termini of recombinant monoclonal antibodies can cyclize spontaneously to pyroglutamate (pE) in vitro. Glutamate conversion to pyroglutamate occurs more slowly than from glutamine but has been observed under near physiological conditions. Here we investigated to what extent human IgG2 N-terminal glutamate converts to pE in vivo. Pyroglutamate levels increased over time after injection into humans, with the rate of formation differing between polypeptide chains. These changes were replicated for the same antibodies in vitro under physiological pH and temperature conditions, indicating that the changes observed in vivo were due to chemical conversion not differential clearance. Differences in the conversion rates between the light chain and heavy chain on an antibody were eliminated by denaturing the protein, revealing that structural elements affect pE formation rates. By enzymatically releasing pE from endogenous antibodies isolated from human serum, we could estimate the naturally occurring levels of this post-translational modification. Together, these techniques and results can be used to predict the exposure of pE for therapeutic antibodies and to guide criticality assessments for this attribute.
SPREADER EQUIPMENT FOR ANTI-ICING (1995)

In the development of anti-icing technology, the need to apply a small amount of chemical in a very uniform pattern on the roadway surface was identified. All known manufacturers of spreader equipment were contacted to develop a master list of sources of application equipment and to learn about their testing procedures. It was determined that there are no procedures in the United States for evaluating highway spreader equipment under field conditions. The German government has standards for testing spreader equipment. Also, the European equipment manufacturers have testing procedures for evaluating their own equipment. A testing procedure will be of use to agencies who wish to evaluate available equipment to integrate anti-icing technology into their snow and ice control operations.
Capturing Data Uncertainty in High-Volume Stream Processing (2009)

We present the design and development of a data stream system that captures data uncertainty from data collection to query processing to final result generation. Our system focuses on data that is naturally modeled as continuous random variables. For such data, our system employs an approach grounded in probability and statistical theory to capture data uncertainty and integrates this approach into high-volume stream processing. The first component of our system captures uncertainty of raw data streams from sensing devices. Since such raw streams can be highly noisy and may not carry sufficient information for query processing, our system employs probabilistic models of the data generation process and stream-speed inference to transform raw data into a desired format with an uncertainty metric. The second component captures uncertainty as data propagates through query operators. To efficiently quantify result uncertainty of a query operator, we explore a variety of techniques based on probability and statistical theory to compute the result distribution at stream speed. We are currently working with a group of scientists to evaluate our system using traces collected from the domains of (and eventually in the real systems for) hazardous weather monitoring and object tracking and monitoring.
Recombinant human granulocyte colony‐stimulating factor inhibits the metastasis of hematogenous and non‐hematogenous tumors in mice (1991)

We studied the effects of in vivo administrations of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the metastasis of murine hematogenous and non-hematogenous tumors in spontaneous and experimental metastasis models. Spontaneous lung metastasis caused by intra-footpad injections of B16-BL6 melanoma and Lewis-lung-carcinoma (3LL) cells were inhibited by intravenous (i.v.) and subcutaneous (s.c.) injections of rhG-CSF after excision of the primary tumors. Recombinant hG-CSF significantly inhibited liver metastasis when administered i.v. after i.v. injection of L5178Y-ML25 T-lymphoma cells. Multiple i.v. administration of rhG-CSF after the tumor inoculation prolonged the survival times of mice inoculated i.v. with L5178Y-ML25 lymphoma cells. Recombinant hG-CSF did not directly affect the growth of B16-BL6 and L5178Y-ML25 cells in vitro. During the administration periods, both i.v. and s.c. injections of rhG-CSF increased the number of total white blood cells (WBC) in peripheral blood to approximately 3 times the normal level in normal and tumor-bearing mice. We also found that the administration of rhG-CSF stimulates neutrophils to become cytostatic against these tumor cells. Our results indicate that the injection of rhG-CSF is effective in inhibiting lung and liver metastases by activating neutrophils and increasing cell number.
NADH-Cytochrome b5 Reductase 3 Promotes Colonization and Metastasis Formation and Is a Prognostic Marker of Disease-Free and Overall Survival in Estrogen Receptor-Negative Breast Cancer* (2015)

Metastasis is the main cause of cancer-related deaths and remains the most significant challenge to management of the disease. Metastases are established through a complex multistep process involving intracellular signaling pathways. To gain insight to proteins central to specific steps in metastasis formation, we used a metastasis cell line model that allows investigation of extravasation and colonization of circulating cancer cells to lungs in mice. Using stable isotopic labeling by amino acids in cell culture and subcellular fractionation, the nuclear, cytosol, and mitochondria proteomes were analyzed by LC-MS/MS, identifying a number of proteins that exhibited altered expression in isogenic metastatic versus nonmetastatic cancer cell lines, including NADH-cytochrome b5 reductase 3 (CYB5R3), l-lactate dehydrogenase A (LDHA), Niemann-pick c1 protein (NPC1), and nucleolar RNA helicase 2 (NRH2). The altered expression levels were validated at the protein and transcriptional levels, and analysis of breast cancer biopsies from two cohorts of patients demonstrated a significant correlation between high CYB5R3 expression and poor disease-free and overall survival in patients with estrogen receptor-negative tumors (DFS: p = .02, OS: p = .04). CYB5R3 gene knock-down using siRNA in metastasizing cells led to significantly decreased tumor burden in lungs when injected intravenously in immunodeficient mice. The cellular effects of CYB5R3 knock-down showed signaling alterations associated with extravasation, TGFβ and HIFα pathways, and apoptosis. The decreased apoptosis of CYB5R3 knock-down metastatic cancer cell lines was confirmed in functional assays. Our study reveals a central role of CYB5R3 in extravasation/colonization of cancer cells and demonstrates the ability of our quantitative, comparative proteomic approach to identify key proteins of specific important biological processes that may also prove useful as potential biomarkers of clinical relevance. MS data are available via ProteomeXchange with identifier PXD001391.
Concepts in Genetic Medicine (2007)

Concepts in genetic medicine , Concepts in genetic medicine , کتابخانه دیجیتال جندی شاپور اهواز
A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors. (1988)

We have developed a new procedure for unidirectional DNA deletion with Bal 31 nuclease. This method is based on the use of chimeric adaptors containing double strands of 2'-O-methyl RNA to block against an attack of Bal 31 nuclease. This contributes a wide application to learn the relation of structure-function of genes.
TMPRSS6 rs855791 modulates hepcidin transcription in vitro and serum hepcidin levels in normal individuals (2011)

The iron hormone hepcidin is inhibited by matriptase-2 (MT2), a liver serine protease encoded by the TMPRSS6 gene. Cleaving the bone morphogenetic protein (BMP) coreceptor hemojuvelin (HJV), MT2 impairs the BMP/son of mothers against decapentaplegic homologs (SMAD) signaling pathway, down-regulates hepcidin, and facilitates iron absorption. TMPRSS6 inactivation causes iron-deficiency anemia refractory to iron administration both in humans and mice. Genome-wide association studies have shown that the SNP rs855791, which causes the MT2 V736A amino acid substitution, is associated with variations of serum iron, transferrin saturation, hemoglobin, and erythrocyte traits. In the present study, we show that, in vitro, MT2 736(A) inhibits hepcidin more efficiently than 736(V). Moreover, in a genotyped population, after exclusion of samples with iron deficiency and inflammation, hepcidin, hepcidin/transferrin saturation, and hepcidin/ferritin ratios were significantly lower and iron parameters were consistently higher in homozygotes 736(A) than in 736(V). Our results indicate that rs855791 is a TMPRSS6 functional variant and strengthen the idea that even a partial inability to modulate hepcidin influences iron parameters and, indirectly, erythropoiesis.
Naturally Aligned Nanocrystals (2000)

The aggregation of nanocrystals has long been believed to result in disordered solids. In his Perspective, Alivisatos discusses recent evidence that nanocrystals may also form oriented assemblies. He highlights the work by Banfield et al. in this issue, who show that such alignment can also occur in natural systems. The results may be of importance not only for geochemistry but also for the synthesis of advanced artificial materials.
Boron-Doped Anatase TiO2: Pure and Hybrid DFT Calculations (2008)

We report the results of density functional theory calculations on the electronic structure of boron-doped anatase TiO2. For the calculations we used both standard and hybrid exchange-correlation functionals. On the basis of a close comparison of computed and measured observable properties, in particular hyperfine coupling constants from electron paramagnetic resonance experiments and core level binding energies from X-ray photoelectron spectroscopy spectra, we propose the existence of both substitutional to oxygen and interstitial boron atoms in the bulk of anatase. Boron substitional to oxygen results in a paramagnetic defect, [BTi3]•, which introduces new states in the midgap of the material. The analysis of the stability of this center, however, shows that it should not be the dominant species and should convert into interstitial boron after annealing at high temperature. Various possible sites for interstitial boron have been considered where the dopant is coordinated to three, [BO3], or to four, [BO4], oxygen atoms. The electronic characteristics of interstitial boron are rather independent of the site where the atom is incorporated. Boron in interstitial positions behaves as a three-electron donor with formation of B3+ and reduction of Ti4+ to Ti3+. The possible copresence of substitutional and interstitial boron could also result in internal charge transfer leading to more complex situations.
Limited Duration of Remission of Insulin Dependency in Children With Recent Overt Type I Diabetes Treated With Low-Dose Cyclosporin (1990)

Preliminary data from our group indicated that cyclosporin A induced frequent remissions of insulin dependency in a group of 40 insulin-dependent (type I) diabetic children if given at the onset of clinical manifestations of diabetes. We report a 2-yr analysis of the response to cyclosporin A in the group of 81 patients included in the initial study. As observed before, a remission could be obtained in most of the patients (65%) in association with a shorter duration of symptoms, less severe hyperglycemia, lower incidence of ketoacidosis, and higher plasma C-peptide concentrations. All remissions ended during the follow-up period after a mean +/- SE duration of 316 +/- 21 days (range 31-850 days). Two parameters were linked to the duration of remissions: the mean circulating level of cyclosporin during the first 3 mo and the duration of prediagnostic polyuria. We were unable to relate the end of a remission to variations in the cyclosporin regimen, titer of autoantibodies, or progression of beta-cell failure. The euglycemic clamp technique revealed that insulin sensitivity decreases with time in patients not taking insulin. At 24 mo, the patients who had a remission of insulin dependency had better glycemic control, lower insulin dosages, and C-peptide levels two- to threefold higher than the nonremission patients and four- to sixfold higher than the historical control subjects. The cyclosporin regimen was well tolerated over the observed period: more specifically, serum creatinine remained unchanged, and kidney biopsies performed at 18-24 mo of treatment were within normal limits.(ABSTRACT TRUNCATED AT 250 WORDS)
Conception de vco a faible bruit de phase en technologie monolithique phemt dans les bandes k et ka (1997)

Ce travail est une contribution a la conception d'oscillateurs accordables a faible bruit de phase, utilisant la technologie monolithique a base de transistors phemt en bande millimetrique. Les systemes de communications actuels exigent la conception d'oscillateurs agiles en frequences et de grande purete spectrale. Ces specifications conduisent a l'adoption de methodologies de conceptions appropriees mettant en uvre les potentialites des simulateurs commerciaux. Ce memoire presente la conception d'un oscillateur controle en tension avec buffer en bande ka (autour de 30ghz). Son bruit de conversion am-fm est optimise par le critere d'orthogonalite. La conception d'un second vco sans buffer en bande k (autour de 24ghz) est basee sur l'optimisation du comportement dynamique du transistor ; l'objectif etant de reduire le bruit de phase genere par les non-linearites. La caracterisation des circuits concus et realises est effectuee. La comparaison entre les resultats obtenus par simulation et les resultats experimentaux montre un accord satisfaisant. La deuxieme partie de ce travail porte sur la modelisation du bruit basses frequences des transistors phemt ainsi que sur l'analyse du bruit de phase des vco. La caracterisation du bruit basses frequences nous a permis d'identifier les differentes composantes du bruit en exces. Un modele de bruit pour la cao des transistors phemt en fonction du courant de drain est propose. Ceci nous a permis de simuler le bruit de phase des vco. L'analyse des resultats de simulation, nous a conduit a constater l'influence du varactor sur le bruit de phase du fait de son coefficient de qualite et de ses non-linearites. Des interpretations expliquant les ecarts entre simulations-mesures sont donnees. Les niveaux du bruit de phase mesures correspondent aux meilleurs resultats releves dans la litterature a ce jour.
Nickel–Thiolate Complex Catalyst Assembled in One Step in Water for Solar H2 Production (2011)

We report the use of a simple complex assembled from Ni(II) salt and 2-mecaptoethanol in one step in water as the efficient catalyst in a molecular hydrogen system which can be sensitized by a low-cost xanthene dye, Erythrosin B. An excellent quantum efficiency of 24.5% is attained at 460 nm. This simple system is expected to contribute toward the development of economical and environmentally benign solar hydrogen production systems.