gebhart/patrepeval huggingface dataset:
A Role for the Interferon‐Inducible Protein 10 in Inhibition of Angiogenesis by Interleukin‐12 (1996)

We have developed a nude mouse model in which tumor regression is reproducibly induced by coinjection with or intratumor inoculation of EBV-immortalized B cells. A wide spectrum of tumor-derived human cell lines can be established as subcutaneous tumors in sublethally irradiated athymic mice. Most of these tumors are induced to regress by human B cells immortalized with EBV. The tumor regression process is characterized by superficial necrosis and scarring that progressively extends itself to involve all or most of the tumor. Microscopically, tumor regression is characterized by tumor tissue necrosis, evidence of vascular damage with intimal thickening and capillary thrombosis, and macrophage, but not lymphocyte or neutrophil, infiltration. Profiles of cytokine expression differed between progressive and regressive Burkitt's tumors in that regressive tumors expressed larger levels of TNF-alpha, IL-6, IFN-gamma, IP-10, Mig, and IL-12 p35, but not other chemokines/cytokines. IP-10 and IL-12 were found to act as potent inhibitors of angiogenesis in vivo. IL-12 is an inducer of IFN-gamma and indirectly of IP-10, an IFN-gamma-inducible protein, raising the possibility that the antiangiogenic effect of IL-12 is mediated by IP-10. Previous studies have demonstrated that IL-12 has potent antitumor activity in vivo. Much of this activity was dependent on the presence of IFN-gamma and of immune T cells. The observation, made in our studies, that IP-10 is an inhibitor of angiogenesis raises the possibility that IP-10 might contribute to the antitumor effects of IL-12 by inhibiting angiogenesis. Inhibition of angiogenesis by IP-10 and IL-12 is T-cell independent, suggesting that IL-12 targets at least two compartments, T cells and capillaries, each capable of mediating antitumor effects.
Influence of Turkey Herpesvirus Vaccination on the B-haplotype Effect on Marek's Disease Resistance in 15.B-congenic Chickens (1992)

Eight recently developed 15.B congenic lines of chickens were tested for Marek's disease (MD) resistance by intra-abdominal injection of cell-associated preparations of MD virus of a virulent strain (JM), a very virulent strain (Md5), or Md5 after vaccination with turkey herpesvirus (HVT) strain FC126. Chickens of the 15.N congenic line (B15B21 or B21B21) were very resistant to JM-induced MD, in contrast to chickens homozygous for the B-haplotypes 2, 5, 12, 13, 15, or 19. After Md5 infection, more than 88% of the chickens in all of the congenic lines developed MD. However, when chickens were vaccinated with HVT before being inoculated with Md5, the B5 and B12 homozygotes were more resistant to MD than were the B2, B13, or B19 homozygotes, and B15 and B21 homozygotes had intermediate resistance. B5B5 and B2B5 F2 chicks inoculated with HVT and Md5 had a lower prevalence of MD than B2B2 sibs. These results demonstrate that a protocol involving HVT vaccination of chicks followed by infection with very virulent MD virus will allow the detection of B-haplotypes determining MD resistance, some of which are not detectable in unvaccinated chicks challenged with virulent MD.
Medicaid and indigent care issue brief: Medicaid: provider reimbursement: year end report-2003. (2003)

Medicaid provider reimbursement rates have been a hot topic for the last several years. Thirty states, including California, Hawaii, Indiana, Maryland, Mississippi, New York and Wisconsin, addressed the issue in the 2001 legislative session while 33 states enacted legislation dealing with provider reimbursement in 2002.
Inhibitory effects of caffeic acid phenethyl ester on the activity and expression of cyclooxygenase-2 in human oral epithelial cells and in a rat model of inflammation. (1999)

We investigated the mechanisms by which caffeic acid phenethyl ester (CAPE), a phenolic antioxidant, inhibited the stimulation of prostaglandin (PG) synthesis in cultured human oral epithelial cells and in an animal model of acute inflammation. Treatment of cells with CAPE (2.5 microg/ml) suppressed phorbol ester (12-O-tetradecanoylphorbol-13-acetate; TPA) and calcium ionophore (A23187)-mediated induction of PGE2 synthesis. This relatively low concentration of CAPE did not affect amounts of cyclooxygenase (COX) enzymes. CAPE nonselectively inhibited the activities of baculovirus-expressed hCOX-1 and hCOX-2 enzymes. TPA- and A23187-stimulated release of arachidonic acid from membrane phospholipids was also suppressed by CAPE (4-8 microg/ml). Higher concentrations of CAPE (10-20 microg/ml) suppressed the induction of COX-2 mRNA and protein mediated by TPA. Transient transfections using human COX-2 promoter deletion constructs were performed; the effects of TPA and CAPE were localized to a 124-bp region of the COX-2 promoter. In the rat carrageenan air pouch model of inflammation, CAPE (10-100 mg/kg) caused dose-dependent suppression of PG synthesis. Amounts of COX-2 in the pouch were markedly suppressed by 100 mg/kg CAPE but were unaffected by indomethacin. These data are important for understanding the anticancer and anti-inflammatory properties of CAPE.
Multinode Acoustic Focusing for Parallel Flow Cytometry (2012)

Flow cytometry can simultaneously measure and analyze multiple properties of single cells or particles with high sensitivity and precision. Yet, conventional flow cytometers have fundamental limitations with regards to analyzing particles larger than about 70 μm, analyzing at flow rates greater than a few hundred microliters per minute, and providing analysis rates greater than 50,000 per second. To overcome these limits, we have developed multinode acoustic focusing flow cells that can position particles (as small as a red blood cell and as large as 107 μm in diameter) into as many as 37 parallel flow streams. We demonstrate the potential of such flow cells for the development of high throughput, parallel flow cytometers by precision focusing of flow cytometry alignment microspheres, red blood cells, and the analysis of a CD4+ cellular immunophenotyping assay. This approach will have significant impact toward the creation of high throughput flow cytometers for rare cell detection applications (e.g., circulating tumor cells), applications requiring large particle analysis, and high volume flow cytometry.
Fault-tolerant quantum computation with asymmetric Bacon-Shor codes (2013)

We develop a scheme for fault-tolerant quantum computation based on asymmetric Bacon-Shor codes, which works effectively against highly biased noise dominated by dephasing. We find the optimal Bacon-Shor block size as a function of the noise strength and the noise bias, and estimate the logical error rate and overhead cost achieved by this optimal code. Our fault-tolerant gadgets, based on gate teleportation, are well suited for hardware platforms with geometrically local gates in two dimensions.
Integrated Microfluidic Compact Disc Device with Potential Use in Both Centralized and Point-of-Care Laboratory Settings (2005)

Reducing the size of reaction volumes in immunodiagnostic procedures can reduce not only reagent cost but also analysis time. With reduced sample and reagent volumes and use of a flow-through mode, reactions reach completion rapidly, potentially decreasing turnaround time and making such systems suitable for point-of-care testing. However, decreased reaction volumes typically lead to other problems, such as imprecision of liquid metering, liquid evaporation, and problems related to the increased surface-to-volume ratio.

To address these challenges, we have developed a new bioanalytical system composed of a disposable microfluidic compact disc (CD) device (Gyrolab Bioaffy™) that is intended for sandwich immunoassays and an instrument for automatic processing of CDs (Gyrolab™ Workstation).

The Gyrolab Bioaffy CD (diameter, 120 mm) contains 104 parallel microstructures conveniently fitting into the CD, each covering a surface area of ∼2 × 15 mm. The CD is manufactured by injection molding using a cycloolefin polymer. The CD is covered with a lid in which appropriately placed holes enable liquid introduction by the robot into the microstructures; the lid protects liquids from evaporation once in the CD. Liquid movement and localization are achieved by a combination of capillary action, centrifugal force, and hydrophobic barriers within the microstructure. Each microstructure contains a 15-nL particle-based column coupled with streptavidin in which reactions take place (Fig. 1⇓ ).

The Gyrolab Bioaffy is designed to be an open system; i.e., it is compatible with various assays that use biotinylated reagents, which bind to the streptavidin column …
MHC Class-I Transgenic Mice (1991)

The introduction of cloned genes into the germline of mice has been proven to be a powerful tool to investigate the role of the respective gene products within the immune system. Here we summarize the transgenic mouse models that have been established with major histocompatibility complex (MHC) class-I genes. Foreign class-I alleles can be expressed in transgenic mice according to their normal expression patterns as authentic self molecules and can function in T-cell responses in the same way as endogenous class-I molecules. Since this is also true for most of the introduced human HLA class-I alleles, there is great interest in establishing mouse models for HLA-linked diseases. A new field of experimental approaches concerning self-tolerance has been opened by tissue specific expression of MHC antigens under specific promoters. Besides negative selection in the thymus, peripheral mechanisms could be identified that induce and maintain self-tolerance.
Evaluating certification authority security (2002)

A growing number of applications in the Internet are making use of X.509 public key certificates. Examples include security protocols such as SSL (used in web browsers), IPsec (used in firewalls and desktop computers), S/MIME (a secure e-mail protocol), and SET (the electronic commerce credit card transaction protocol). The public key certificates employed by the applications are created by Certification Authorities (CAs), that vouch for the binding of various attributes (e.g., identity) to a public key. Thus security of these applications is dependent on the security of the CA function. This paper examines security for CAs. It begins with a characterization of security requirements for CAs and continues with an exploration of the wide range of attacks that can be mounted against CAs. Included are attacks against network communications, against the operating systems used by CAs, "close-in" technical attacks against CA components (including cryptographic modules), and even misbehavior by human operators. The paper concludes with an examination of three approaches to implementing CA cryptographic support functions, analyzing each relative to the attack scenarios developed earlier in the paper.
Maternal Iron Deficiency and the Risk of Schizophrenia in Offspring (2008)

The findings suggest that maternal iron deficiency may be a risk factor for SSDs among offspring. Given that this hypothesis offers the potential for reducing the risk for SSDs, further investigation in independent samples is warranted.
AN INVESTIGATION OF THE ACCURACY OF PARALLAX BAR HEIGHTING BY MEANS OF NUMERICAL SIMULATION (1987)

Abstract Previous assessments of the accuracy of parallax bar heighting were handicapped by small sample sizes. Numerical simulation was avoided due to the difficulty of obtaining co‐ordinates for the conjugate principal points. This problem has been overcome, however, and all required photograph coordinates have been rigorously computed following the generation of fictitious data for ground points and exposure stations. The parallax bar heighting process has additionally been simulated in order to investigate both external factors, such as image errors, tilts and ground relief, and also influences intrinsic in the heighting process, for example estimation of flying height, baselining, control configuration and error surface model.
Mouse models of human disease. Part I: techniques and resources for genetic analysis in mice. (1997)

The mouse is an ideal model organism for human disease. Not only are mice physiologically similar to humans, but a large genetic reservoir of potential models of human disease has been generated through the identification of >1000 spontaneous, radiation- or chemically induced mutant loci. In addition, a number of recent technological advances have dramatically increased our ability to create mouse models of human disease. These technological advances include the development of highresolution genetic and physical linkage maps of the mouse genome, which in turn are facilitating the identification and cloning of mouse disease loci. Furthermore, transgenic technologies that allow one to ectopically express or make germ-line mutations in virtually any gene in the mouse genome have been developed, as well as methods for analyzing complex genetic diseases. In Part I of this review, we summarize some of the classical and modern approaches that have fueled the recent dramatic explosion in mouse disease model development. In Part II of this review, we list >100 mouse models of human disease where the homologous gene has been shown to be mutated in both human and mouse (Bedell et al., this issue). In the vast majority of these models, the mouse mutant phenotype very closely resembles the human disease phenotype and these models therefore provide valuable resources to understand how the diseases develop and to test ways to prevent or treat these diseases. Additionally, we highlight a number of areas of this research where significant progress has been made in the past few years.
Hot-Pressing of Bi-Pb-Sr-Ca-Cu-O Superconducting Thick Film (1990)

Screen-printed Bi 1.84 Pb 0.34 Sr 2.03 Ca 1.9 Cu 3.06 O y superconducting thick films were prepared on MgO substrates from powder consisting almost entirely of the 110 K phase, and the thick film sandwiched between the two MgO substrates was further hot-pressed in air. Under the hot-pressing condition of 500 kg/cm 2 at 855°C for 30 min, the T c and resistivity at 270 K were 105 K and 6.1×10 -4 Ω·cm, respectively. The J c was 4800 A/cm 2 at 77 K in zero magnetic field. The relationship between superconducting properties and hot-pressing conditions was examined.
Iterative Joint Estimation Procedure for Channel and Frequency Offset in Multi-Antenna OFDM Systems With an Insufficient Cyclic Prefix (2013)

This paper addresses a strategy to improve the joint channel and frequency offset (FO) estimation in multi-antenna systems, widely known as multiple-input–multiple-output orthogonal frequency-division multiplexing (MIMO-OFDM), in the presence of intersymbol interference (ISI) and intercarrier interference (ICI) occasioned by an insufficient cyclic prefix (CP). The enhancement is attained by the use of an iterative joint estimation procedure (IJEP) that successively cancels the interferences located in the preamble of the OFDM frame, which is used for the joint estimation and initially contains the interferences due to a CP shorter than the channel length. The IJEP requires at certain steps a proper iterative interference cancellation algorithm, which makes use of an initial FO compensation and channel estimation obtained due to the use of a symmetric sequence in the preamble. After the iterative cancellation of interferences, the procedure performs an additional joint channel and FO estimation whose mean square error converges to the Cramér–Rao bound (CRB). Later on, this subsequent joint estimation permits the removal of the interferences in the data part of the frame, which are also due to an insufficient CP, in the same iterative fashion but saving iterations compared with the use of other estimation strategies. The appraisal of the procedure has been performed by assessing the convergence of the simulated estimators to the CRB as a function of the number of iterations. Additionally, simulations for the evaluation of the bit error rate (BER) have been carried out to probe how the utilization of the proposed IJEP clearly improves the performance of the system. It is concluded that, with a reduced number of iterations in the preamble, the IJEP converges to the theoretical bounds, thus reducing the disturbances caused by a hard wireless channel or a deliberately insufficient CP. Therefore, this eases the interference cancellation in the data part, leading to an improvement in the BER that approximates to the ideal case of a sufficient CP and, consequently, an improvement in the computational cost of the whole procedure that has been analyzed.
miRNA expression profiles in cerebrospinal fluid and blood of patients with Alzheimer’s disease and other types of dementia – an exploratory study (2016)

Detection of miRNA expression profiles in blood and in particular CSF of patients diagnosed with different types of dementia is feasible and it seems that several expressional differences between AD and other dementia types do exist when measured in a clinically relevant setup. In this explorative pilot study, the deregulated miRNAs in CSF of AD patients may be associated with relevant target genes related to AD pathology, including APP and BACE1, which suggests that miRNAs are interesting candidates for AD biomarkers in the future.
High rate etching of SiC using inductively coupled plasma reactive ion etching in SF6-based gas mixtures (1999)

Inductively coupled plasma (ICP) reactive ion etching of SiC was investigated using SF6 plasmas. Etch rates were studied as a function of substrate bias voltage (−3 to −500 V), ICP coil power (500–900 W), and chamber pressure (1–6 mT). The highest etch rate (970 nm/min) for SiC yet reported was achieved. Anisotropic etch profiles with highly smooth surfaces free of micromasking effects were obtained. The addition of O2 to the SF6 plasma was found to slightly increase the etch rate.
Indoleamine‐mediated reciprocal modulation of on‐centre and off‐centre ganglion cell activity in the retina of the cat. (1984)

The effects of applying the indoleamines serotonin (5-hydroxytryptamine) and 5-methoxy-N,N-dimethyltryptamine (MDT) ionophoretically onto cat retinal ganglion cells were studied in the optically intact eye of the cat. Serotonin suppressed both the spontaneous activity and light-evoked discharge of on-centre ganglion cells, regardless of the visual stimulus used; on the other hand, it enhanced the activity of off-centre ganglion cells. MDT produced the opposite effects, i.e. it enhanced the activity of on-centre ganglion cells but suppressed that of off-centre ganglion cells. Much of the effect of serotonin on light-evoked discharge can be attributed to an alteration of maintained discharge. There was no major difference in the way brisk-sustained (X) and brisk-transient (Y) cells responded to the application of serotonin and MDT, although the effects of the indoleamines on brisk-transient (Y) cells were generally weaker than on brisk-sustained (X) cells. In view of the fact that the action of serotonin is similar to the action of a gain control system, a possible contribution of indoleamine-accumulating neurones to gain control in the cat retina is discussed.
ChemInform Abstract: Synthesis of Fluorescent or Biotinylated Nucleoside Compounds. (1987)

Abstract The preparation is described of two nucleosides bearing non‐radioactive (chemical) labels, the fluorescent pyrimidine nucleoside (IIIb) and the biotinylated adenosine 5'‐triphosphate (IXb).
Application of high-pressure Raman spectroscopy to bone biomechanics (2003)

Compressive stress on bone, cartilage and other tissues accompanies normal activity. While the biomechanical properties of many tissues are reasonably well-understood at many levels of structure, surprisingly little is known at the ultrastructural and crystal lattice levels. We show how the use of diamond anvil cell Raman microspectroscopy enables a deeper understanding of the response of tissue to mechanical stress. We discuss the reversible responses of deproteinated and intact bone powders to hydrostatic pressure and compare these responses to those of a model compound, synthetic carbonated apatite.
Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. (1990)

The polymerase chain reaction (PCR) was used to amplify viral or oncogene sequences from frozen or formalin-fixed, paraffin-embedded tissue sections. Methods for preparing fixed, embedded colonic tissue for PCR amplification of c-K-ras sequences from genomic DNA and for amplification of viral DNA from other tissues, including brain, lung, and liver, were evaluated. The effect of formalin fixation on the efficiency of amplification was also determined. While there seemed to be only a modest variation in the efficiency of the PCR for amplification of single-copy human genes, regardless of the methods used for tissue preparation, amplification of viral DNA sequences against a human genomic DNA background was more efficient when the DNA was purified to some degree before amplification of the tissue. We used the PCR to examine frozen and fixed embedded tissue sections for the presence of Epstein-Barr virus (EBV) and cytomegalovirus (CMV) DNA. One patient with a heart-lung transplant succumbed to a lymphoproliferative disorder, and EBV genome was present in tissues with abnormal lymphoid infiltrates. CMV was also present in bronchial lavages from the same patient, where cytologic diagnosis was not apparent. Another patient with a liver transplant showed CMV genome in multiple liver biopsies, with negative histologic results for CMV. In vitro DNA amplification with the PCR demonstrated sensitivity superior to that of histology in detecting CMV and EBV in the cases examined.
Trp RNA-Binding Attenuation Protein: Modifying Symmetry and Stability of a Circular Oligomer (2012)

We conclude that the oligomeric state of a circular protein can be changed by introducing a point mutation at a subunit interface. Exclusion (or deletion) of the C-terminus from the subunit interface has a major impact on properties of TRAP oligomers, making them more stable, and we argue that the cause of these changes is the altered oligomeric state. The more stable TRAP oligomers could be used in potential applications of TRAP in bionanotechnology.
Joint coding-precoding with low-complexity turbo-decoding (2004)

We combine error-control coding with linear precoding (LP) for flat-fading channels, as well as for wireless orthogonal frequency-division multiplexing transmissions through frequency-selective fading channels. The performance is analyzed and compared with the corresponding error-control-coded system without precoding. By wedding LP with conventional error-control coding, the diversity order becomes equal to the error-control code's minimum Hamming distance times the precoder size. We also derive a low-complexity turbo-decoding algorithm for joint coded-precoded transmissions. We analyze the decoding complexity and compare it with an error-control-coded system without LP. Extensive simulations with convolutional and turbo codes for HiperLan/2 channels support the analysis and demonstrate superior performance of the proposed system.
Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies (2020)

We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.
A new HTLV-III/LAV Encoded Antigen Detected by Antibodies from AIDS Patients (1985)

A newly identified protein from HTLV-III/LAV, the virus implicated as the etiologic agent of the acquired immune deficiency syndrome, was studied. This protein, which has a molecular weight of 27,000 (p27), was shown by amino acid sequencing to have a coding origin 3' to the env gene on the HTLV-III genome. The presence of antibodies to p27 in virus-exposed individuals indicated that this gene is functional in the natural host.