gebhart/patrepeval huggingface dataset:
Overexpression of Protein Phosphatase 4 Correlates with Poor Prognosis in Patients with Stage II Pancreatic Ductal Adenocarcinoma (2012)

Our study showed that PP4C is an independent prognostic factor in patients with stage II PDAC.
Normobaric hyperoxia attenuates early blood–brain barrier disruption by inhibiting MMP‐9‐mediated occludin degradation in focal cerebral ischemia (2008)

Early blood-brain barrier (BBB) disruption resulting from excessive neurovascular proteolysis by matrix metalloproteinases (MMPs) is closely associated with hemorrhagic transformation events in ischemic stroke. We have shown that normobaric hyperoxia (NBO) treatment reduces MMP-9 increase in the ischemic brain. The aim of this study was to determine whether NBO could attenuate MMP-9-mediated early BBB disruption following ischemic stroke. Rats were exposed to NBO (95% O(2)) or normoxia (30% O(2)) during 90-min middle cerebral artery occlusion, followed by 3-hour reperfusion. NBO-treated rats showed a significant reduction in Evan's blue extravasation in the ischemic hemisphere compared with normoxic rats. Topographically, Evan's blue leakage was mainly seen in the subcortical regions including the striatum, which was accompanied by increased gelatinolytic activity and reduced immunostaining for tight-junction protein, occludin. Increased gelatinolytic activities and occludin protein loss were also observed in isolated ischemic microvessels. Gel gelatin zymography identified that MMP-9 was the main enzymatic source in the cerebral microvessels. Incubation of brain slices or isolated microvessels with purified MMP-9 revealed specific degradation of occludin. Inhibition of MMP-9 by NBO or MMP-inhibitor, BB1101, significantly reduced occludin protein loss in ischemic microvessels. These results suggest that NBO attenuates early BBB disruption, and inhibition of MMP-9-mediated occludin degradation is an important mechanism for this protection.
Time-dependent resonant tunneling via two discrete states (1996)

We theoretically investigate time-dependent resonant tunneling via two discrete states in an experimentally relevant setup. Our results show that the dc transport through the system can be controlled by applying external irradiation with a frequency which matches the energy difference between the discrete states. We predict resonant phenomena which should be easily observable in experiments. \textcopyright{} 1996 The American Physical Society.
Adenosine Receptor Antagonists:  Translating Medicinal Chemistry and Pharmacology into Clinical Utility (2008)

The first recorded report describing evidence for an AR originates from 1976. Now, 30 years later, advances in understanding the role of adenosine and its receptors in physiology and pathophysiology as well as new developments in medicinal chemistry of these receptors have enabled researchers to identify potential therapeutic areas for drug development. With the combination of pharmacological data, using selective ligands and genetically modified mice, important progress has been made toward an understanding of the role of ARs in a variety of diseases, such as inflammatory conditions, sepsis, heart attack, ischemia-reperfusion injury, vascular injury, spinal cord injury, chronic obstructive pulmonary disease (COPD), asthma, diabetes, obesity, inflammatory bowel disease, retinopathy, and Parkinson’s Disease (PD). Nonselective AR antagonists are used to maintain wakefulness (caffeine) and, less commonly at present, treat bronchospasm (theophylline, aminophylline, enprofylline). Currently a number of new selective AR agonists and antagonists are in testing for a variety of new indications. Therefore, the purpose of this review is to analyze the structure-activity relationships of the ligands synthesized as antagonists for the ARs. We have included some synthetic schemes in order to show the chemistry involved in this field. In particular, we will investigate antagonists under active development that selectively target the four known AR subtypes.
Interleukin 2 induced leukocyte adhesion to the normal and tumor microvascular endothelium in vivo and its inhibition by dextran sulfate: implications for vascular leak syndrome. (1991)

The maximum dose of interleukin 2 (IL-2) alone or with adoptively transferred lymphocytes is limited by the vascular leak syndrome, resulting from an increase in vascular permeability. Using intravital microscopy to quantify cell interaction in vivo and the Miles assay to assess changes in vascular permeability, we have shown that IL-2 increases both leukocyte-endothelial cell adhesion and vascular permeability in the cutaneous tissue of rabbits. Both of these processes can be reversed to a large extent using dextran sulfate (Mr approximately 500,000; 10 mg/kg body weight), a nonspecific blocker of leukocyte-endothelial adhesion. These results suggest that increased leukocyte (probably lymphocyte) adhesion to the postcapillary microvasculature contributes significantly to the IL-2 induced increase in permeability. Therefore, as more specific inhibitors of leukocyte-endothelial adhesion become available, improved strategies could be developed to control or prevent IL-2 toxicity.
Genetically engineered live attenuated influenza A virus vaccine candidates (1997)

We have generated new influenza A virus live attenuated vaccine candidates by site-directed mutagenesis and reverse genetics. By mutating specific amino acids in the PB2 polymerase subunit, two temperature-sensitive (ts) attenuated viruses were obtained. Both candidates have 38 degrees C shutoff temperatures in MDCK cells, are attenuated in the respiratory tracts of mice and ferrets, and have very low reactogenicity in ferrets. Infection of mice or ferrets with either mutant conferred significant protection from challenge with the homologous wild-type virus. Three tests for genetic stability were used to assess the propensity for reversion to virulence: 14 days of replication in nude mice, growth at 37 degrees C in tissue culture, and serial passage in ferrets. One candidate, which contains mutations intended to reduce the ability of PB2 to bind to cap structures, was stable in all three assays, whereas the second candidate, which contains mutations found only in other ts strains of influenza virus, lost its ts phenotype in the last two assays. This approach has therefore enabled the creation of live attenuated influenza A virus vaccine candidates suitable for human testing.
A phase 1/2 trial of arginine butyrate and ganciclovir in patients with Epstein-Barr virus–associated lymphoid malignancies (2006)

Malignancies associated with latent Epstein-Barr virus (EBV) are resistant to nucleoside-type antiviral agents because the viral enzyme target of these antiviral drugs, thymidine kinase (TK), is not expressed. Short-chain fatty acids, such as butyrate, induce EBV-TK expression in latently infected B cells. As butyrate has been shown to sensitize EBV(+) lymphoma cells in vitro to apoptosis induced by ganciclovir, arginine butyrate in combination with ganciclovir was administered in 15 patients with refractory EBV(+) lymphoid malignancies to evaluate the drug combination for toxicity, pharmacokinetics, and clinical responses. Ganciclovir was administered twice daily at standard doses, and arginine butyrate was administered by continuous infusion in an intrapatient dose escalation, from 500 mg/(kg/day) escalating to 2000 mg/(kg/day), as tolerated, for a 21-day cycle. The MTD for arginine butyrate in combination with ganciclovir was established as 1000 mg/(kg/day). Ten of 15 patients showed significant antitumor responses, with 4 CRs and 6 PRs within one treatment cycle. Complications from rapid tumor lysis occurred in 3 patients. Reversible somnolence or stupor occurred in 3 patients at arginine butyrate doses of greater than 1000 mg/(kg/day). The combination of arginine butyrate and ganciclovir was reasonably well-tolerated and appears to have significant biologic activity in vivo in EBV(+) lymphoid malignancies which are refractory to other regimens.
Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys (2016)

Zika virus (ZIKV) is responsible for a major ongoing epidemic in the Americas and has been causally associated with fetal microcephaly. The development of a safe and effective ZIKV vaccine is therefore an urgent global health priority. Here we demonstrate that three different vaccine platforms protect against ZIKV challenge in rhesus monkeys. A purified inactivated virus vaccine induced ZIKV-specific neutralizing antibodies and completely protected monkeys against ZIKV strains from both Brazil and Puerto Rico. Purified immunoglobulin from vaccinated monkeys also conferred passive protection in adoptive transfer studies. A plasmid DNA vaccine and a single-shot recombinant rhesus adenovirus serotype 52 vector vaccine, both expressing ZIKV premembrane and envelope, also elicited neutralizing antibodies and completely protected monkeys against ZIKV challenge. These data support the rapid clinical development of ZIKV vaccines for humans.
Chromosome 18q deletion and Smad4 protein inactivation correlate with liver metastasis: a study matched for T- and N- classification (2006)

Smad4 protein, whose gene is coded at chromosome 18q21.1, is an important tumour suppressor that mediates transforming growth factor-beta. It has been reported that inactivation of the Smad4 gene and allelic loss of chromosome 18q correlate with liver metastasis and poorer prognosis in colorectal cancers. Utilising a recently developed method of immunohistochemical staining for Smad4 protein, we focused on the specific impact of Smad4 protein expression on liver metastasis in colorectal cancer. We also evaluated the association between chromosome18q deletion and liver metastasis. We selected 20 colorectal cancers with liver metastasis for the experimental group, and 20 cases without liver metastasis for the control. In order to exclude the influence of lymph node metastasis, all cases were lymph node negative. In addition, the two groups were matched for tumour depth, tumour differentiation and tumour location. We compared the expression level of Smad4 protein immunohistochemically in these 20 matched pairs. We also compared the loss of heterozygosity status at chromosome 18q in these 20 matched pairs. Immunohistochemical staining revealed a significant difference (P = 0.024) in the level of Smad4 protein between the two groups. We also observed a significantly different (P=0.0054) ratio of allelic deletion at chromosome 18q21. Smad4 protein expression level and allelic loss at 18q21 are associated with the process of liver metastasis in colorectal cancers evaluated when excluding clinical and pathological features except for liver metastasis.
Pump Performance Analyses with High Viscous Fluids (2004)

In this study the effects of fluid viscosity on the pump performances for a conventional centrifugal pump were experimentally investigated. The study aimed to compare the pump characteristics between water and viscosity fluids. In order to measure the flow rate and pressure, v-notch weir and bourdon pressure gauges were used for the codes of KS B6301 and KS B6302. The working fluids were water, aqueous sugar and glycerin solutions. The results were summarized as follows ; The experimental results were summarized as follows : the pump characteristics of the total head, shaft power, and efficiency with high viscosity fluids were different from those of water. When the viscosity of the applied fluid was increased, the total head and efficiency were more decreased than those of water. The decreasing gradients of the total head and the efficiency were larger than water due to the increased disk friction losses at the duty operation point. However, the shut-off head was almost constant regardless the viscosity of applied fluids. Each efficiency curves for the sugar 20w% and glycerin 20w% solutions was decreased up to 15.1% and 34.4% than that of water, respectively.
THE PHOTOBIOLOGICAL BASIS OF LOW LEVEL LASER RADIATION THERAPY (1991)

Low level laser radiation therapy is effective in a number of clinical situations (e,g. pain relief, wound healing, sports medicine), but the photobiological basis of this therapy is not well-understood, Since both visible and infrared radiations have been shown to be beneficial in such therapies, and since these two radiations differ dramatically in their photochemical and photophysical properties, how can they produce similar results clinically? I propose a modification of the model of Karu1 to explain this. In her model. visible light produces photochemical changes in photoreceptors in the mitochondria, which alter metabolism, which leads to signal transduction to other parts of the cell (including membranes), which finally leads to the photoresponse (i.e, biostimulation). While visible light probably starts the cascade of metabolic events at the level of the respiratory chain of the mitochondria through photochemical events (probably the photoactivation of enzymes), I propose that because of the photochemical and photophysical properties of infrared radiation, infrared radiation starts the cascade of metabolic events by photophysical effects on the membranes (probably the Ca++ channels), Action spectra are needed to quantitate the relative effectiveness of the different wavelengths of radiation, since this can help to identify the photoreceptors for the photobiological response, and to establish the optimum conditions (i,e. wavelength, dose, and treatment schedule) for a particular therapy,
Antigens Induced on Erythrocytes by P. falciparum : Expression of Diverse and Conserved Determinants (1986)

Red blood cells that are infected with the malaria parasite Plasmodium falciparum express new antigens on their surface. In a study of these antigens in the erythrocytes of naturally infected children in the Gambia, an antibody-mediated agglutination assay revealed an extreme degree of antigenic diversity. Serum samples from each of ten children in the convalescent stage of malaria infection reacted with infected cells from the same child but generally not with infected cells from the other children. The Gambian children's erythrocytes also expressed shared determinants: sera from Gambian adults often reacted with the surface of infected cells from all of the children and were shown by adsorption and elution experiments to contain antibodies that recognized several isolates. Conserved determinants exposed on infected erythrocytes may be important for development of antimalarial immunity either naturally or through vaccination.
Device-free gesture tracking using acoustic signals (2016)

Device-free gesture tracking is an enabling HCI mechanism for small wearable devices because fingers are too big to control the GUI elements on such small screens, and it is also an important HCI mechanism for medium-to-large size mobile devices because it allows users to provide input without blocking screen view. In this paper, we propose LLAP, a device-free gesture tracking scheme that can be deployed on existing mobile devices as software, without any hardware modification. We use speakers and microphones that already exist on most mobile devices to perform device-free tracking of a hand/finger. The key idea is to use acoustic phase to get fine-grained movement direction and movement distance measurements. LLAP first extracts the sound signal reflected by the moving hand/finger after removing the background sound signals that are relatively consistent over time. LLAP then measures the phase changes of the sound signals caused by hand/finger movements and then converts the phase changes into the distance of the movement. We implemented and evaluated LLAP using commercial-off-the-shelf mobile phones. For 1-D hand movement and 2-D drawing in the air, LLAP has a tracking accuracy of 3.5 mm and 4.6 mm, respectively. Using gesture traces tracked by LLAP, we can recognize the characters and short words drawn in the air with an accuracy of 92.3% and 91.2%, respectively.
Mutations in Toll-Like Receptor 3 Are Associated with Elevated Levels of Rotavirus-Specific IgG Antibodies in IgA-Deficient but Not IgA-Sufficient Individuals (2013)

Double-stranded RNA (dsRNA) triggers immune-mediated responses through toll-like receptor 3 (TLR3), which is involved in innate antiviral defense. Low expression of TLR3 was recently suggested to contribute to susceptibility to rotavirus infection. Thus, we investigated the role of two TLR3 polymorphisms (rs3775291 and rs5743305), both of which resulted in reduced protein function or expression, in healthy blood donors and IgA-deficient (IgAD) individuals. These polymorphisms were associated with elevated rotavirus-specific IgG titers in IgAD individuals but not in healthy individuals. Thus, we propose that TLR3 signaling does not contribute to the rotavirus-specific antibody response in IgA-sufficient individuals, whereas it is associated with elevated antibody titers in IgAD individuals.
Aerobic and anaerobic soil metabolism of dicamba (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAerobic and anaerobic soil metabolism of dicambaJames P. Krueger, Robert G. Butz, and Douglas J. CorkCite this: J. Agric. Food Chem. 1991, 39, 5, 995–999Publication Date (Print):May 1, 1991Publication History Published online1 May 2002Published inissue 1 May 1991https://pubs.acs.org/doi/10.1021/jf00005a039https://doi.org/10.1021/jf00005a039research-articleACS PublicationsRequest reuse permissionsArticle Views280Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated. (1976)

Synthesis of proteins encoded by the R222 plasmid was observed in a DNA-directed cell-free system and the products were compared to those plasmid proteins synthesized in Escherichia coli minicells. A greater number of plasmid-specified proteins was detected in the in vitro system than in the minicell, suggesting the presence of control factors for plasmid gene expression in the minicell. Synthesis of a newly detected plasmid protein (TET protein) is induced by tetracycline in minicells containing tetracycline-resistant plasmids, including R222, and this induced synthesis correlates with induced host resistance to the drug. This TET protein was synthesized in vitro from R222 DNA in the absence of tetracycline, indicating that no positive regulatory role for tetracycline is required for the protein's synthesis. TET proteon synthesis was inhibited in vitro when cell-free extracts prepared from cells containing the R222 plasmid were used.
Mesenchymal stem cell‐based therapy: a new paradigm in regenerative medicine (2009)

Mesenchymal stem cells (MSCs), adherent fibroblastoid cells, present in bone marrow and many other tissues can be easily isolated and expanded in vitro. They are capable of differentiating into different cell types such as osteoblasts, chondrocytes, adipocytes, cardiomyocytes, hepatocytes, endothelial cells and neuronal cells. Such immense plasticity coupled with their ability to modulate the activity of immune cells makes them attractive for stem cell-based therapy aimed at treating previously incurable disorders. Preclinical studies have reported successful use of MSCs for delivering therapeutic proteins and repairing defects in a variety of disease models. These studies highlighted the in vivo potential of MSCs and their ability to home to injury sites and modify the microenvironment by secreting paracrine factors to augment tissue repair. Their therapeutic applicability has been widened by genetic modification to enhance differentiation and tissue targeting, and use in tissue engineering. Clinical trials for diseases such as osteogenesis imperfecta, graft-versus-host disease and myocardial infarction have shown some promise, demonstrating the safe use of both allogeneic and autologous cells. However, lack of knowledge of MSC behaviour and responses in vitro and in vivo force the need for basic and animal studies before heading to the clinic. Contrasting reports on immunomodulatory functions and tumorigenicity along with issues such as mode of cell delivery, lack of specific marker, low survival and engraftment require urgent attention to harness the potential of MSC-based therapy in the near future.
Visfiles (2000)

Arguably the trigger for the success of the World Wide Web in the early 1990s was its ability to convey images as well as text. Documents could include colour pictures at no extra cost. In those days, however, scientific visualization in a Web context was a passive activity. A scientist would create a visualization using a conventional system, and publish it on a website - for a reader to later observe. The Web was simply a publication medium. Today of course the Web represents a rich distributed computing environment: visualization can now be executed as a live process with active engagement from the audience.A new field of Web-based visualization has emerged.
Abundant bacterial expression and reconstitution of an intrinsic membrane-transport protein from bovine mitochondria (1993)

The oxoglutarate carrier, an intrinsic membrane-transport protein of the inner membranes of bovine-heart mitochondria, has been expressed at an abundant level in Escherichia coli. It accumulates in the bacterium as inclusion bodies, and none of the protein was detected in the bacterial inner membrane. The mitochondrial ADP/ATP carrier, a member of the same super-family of transport proteins as the oxoglutarate carrier, has also been expressed in E. coli. However, the expression of the ADP/ATP carrier in bacteria retards their growth, and so the levels of expression that were attained were lower than those of the oxoglutarate carrier. The oxoglutarate carrier inclusion bodies have been disaggregated with the detergent N-dodecanoyl-sarcosine, and the protein has been incorporated into liposomes. In its ability to transport oxoglutarate and malate and other known substrates of the carrier in mitochondria, and in its inhibition characteristics by a wide range of non-competitive and competitive inhibitors, this reconstituted oxoglutarate carrier is similar to the natural protein in the inner membranes of mitochondria, and to the carrier that has been purified from mitochondria and reconstituted in liposomes. These experiments remove significant obstacles to crystallization trials and to site-directed mutagenesis of the oxoglutarate carrier.