gebhart/patrepeval huggingface dataset:
Mitochondrial Parkin Recruitment Is Impaired in Neurons Derived from Mutant PINK1 Induced Pluripotent Stem Cells (2011)

Genetic Parkinson disease (PD) has been associated with mutations in PINK1, a gene encoding a mitochondrial kinase implicated in the regulation of mitochondrial degradation. While the studies so far examined PINK1 function in non-neuronal systems or through PINK1 knockdown approaches, there is an imperative to examine the role of endogenous PINK1 in appropriate human-derived and biologically relevant cell models. Here we report the generation of induced pluripotent stem (iPS) cells from skin fibroblasts taken from three PD patients with nonsense (c.1366C>T; p.Q456X) or missense (c.509T>G; p.V170G) mutations in the PINK1 gene. These cells were differentiated into dopaminergic neurons that upon mitochondrial depolarization showed impaired recruitment of lentivirally expressed Parkin to mitochondria, increased mitochondrial copy number, and upregulation of PGC-1α, an important regulator of mitochondrial biogenesis. Importantly, these alterations were corrected by lentiviral expression of wild-type PINK1 in mutant iPS cell-derived PINK1 neurons. In conclusion, our studies suggest that fibroblasts from genetic PD can be reprogrammed and differentiated into neurons. These neurons exhibit distinct phenotypes that should be amenable to further mechanistic studies in this relevant biological context.
In vivo three-dimensional optical coherence elastography (2011)

We present the first three-dimensional (3D) data sets recorded using optical coherence elastography (OCE). Uni-axial strain rate was measured on human skin in vivo using a spectral-domain optical coherence tomography (OCT) system providing >450 times higher line rate than previously reported for in vivo OCE imaging. Mechanical excitation was applied at a frequency of 125 Hz using a ring actuator sample arm with, for the first time in OCE measurements, a controlled static preload. We performed 3D-OCE, processed in 2D and displayed in 3D, on normal and hydrated skin and observed a more elastic response of the stratum corneum in the hydrated case.
Biochemical Characterization of Junonia coenia Densovirus Nonstructural Protein NS-1 (2002)

Junonia coenia densovirus (JcDNV) is an autonomous parvovirus that infects the larvae of the common buckeye butterfly, Junonia coenia. Unlike vertebrate parvoviruses, the genes encoding the structural protein and nonstructural (NS) proteins of JcDNV are in opposite orientations; thus, each strand contains a sense and antisense open reading frame (ORF). The promoter at map position 93 controls expression of NS ORFs 2, 3, and 4, which encode three NS proteins, NS-1, NS-2, and NS-3. These proteins are likely to be involved in viral DNA replication, among other functions. In contrast to the nonstructural proteins of the vertebrate parvoviruses, the NS proteins of the Densovirinae have not been characterized. Here, we describe biochemical properties of the NS-1 protein of JcDNV. The NS-1 ORF was cloned in frame with the Escherichia coli malE gene, which encodes the bacterial maltose binding protein (MBP). Using electrophoretic mobility shift and DNase I protection assays, we identified the region of the JcDNV terminal sequence that is recognized specifically by the MBP-NS-1 fusion protein. The site consists of (GAC)4 and is located on the A-A' region of the terminal palindrome. In addition, the MBP-NS-1 fusion protein catalyzes the cleavage of single-stranded DNA (ssDNA) substrates derived from the JcDNV putative origin of replication, primarily at two sites in the motif 5'-G*TAT*TG-3'. One cleavage site is between the thymidine dinucleotide at positions 92 and 93 and the other site corresponds to thymidine at nucleotide 95; both sites are on the complementary strand of the sequence assigned GenBank accession number A12984. Cleavage of ssDNA is dependent on the presence of a divalent metal cofactor but does not require nucleoside triphosphate hydrolysis. Parvovirus NS proteins contain the phylogenically conserved Walker A- and B-site ATPase motifs. These sites in JcDNV NS-1 diverge from the consensus, yet despite these atypical motifs our analyses support that MBP-NS-1 has ATP-dependent helicase activity. These results indicate that JcDNV NS-1 possesses activities common to the superfamily of rolling-circle replication initiator proteins in general and the parvovirus replication proteins in particular, and they provide a basis for comparative analyses of the structure and function relationships among the parvovirus NS-1 equivalents.
Soluble fibrin as a molecular marker for a pre-thrombotic state (1993)

Increased plasma levels of soluble fibrin are considered as molecular markers of an impending thrombotic event. Several methods to assess soluble fibrin have been in existence for many years. Most of those methods are nonspecific, semiquantitative or too laborious to be used in clinical practice. More recent methods are quantitative and relatively rapid. Some of those methods may, however, not be fully specific; in other cases clinical experience is lacking.
Chemically amplified resist process for 0.25-μm generation photomasks (1996)

A chemically amplified (CA) negative electron beam resist process is developed for fabricating 0.25 &#956;m devices 4X magnification reticles. In order to improve a critical dimension (CD) uniformity on reticles, a precise temperature controllability in post exposure baking (PEB) is realized. Dry etching for Cr is also adopted for getting an appropriate pattern fidelity down to submicron on reticles to meet requirements for such as optical proximity effect correction. Under the optimized process conditions, a CD uniformity of 0.016 &#956;m in 3&#963; is achieved on reticles. A CD linearity of down to 0.8 &#956;m on the reticles is ensured as well. Actual 0.25 &#956;m device reticle production results show that the CD mean variation is controlled within ± 0.040 &#956;m. It is confirmed that an advanced 4X reticle fabrication process for 0.25 &#956;m device generation is realized.
Life Insurance Financial Management Issues (1987)

J. David Cummtins. in his excellent ARIA Presidential Address 161, noted tIle need to link theory and practice in risk and insurance research. [hle life insurance industry is encountering a wide variety ol regulatory anid operatiottal problems hiat will be dealt wilit successfully only by usittg the best actuarial aind tinancial research to develop realistic applied solutions. li this regard, Cummtins' observations could not heave been more titiely.
The agglutination of erythrocytes by influenza virus is strongly inhibited by liposomes incorporating an analog of sialyl gangliosides (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe agglutination of erythrocytes by influenza virus is strongly inhibited by liposomes incorporating an analog of sialyl gangliosidesJill E. Kingery-Wood, Kevin W. Williams, George B. Sigal, and George M. WhitesidesCite this: J. Am. Chem. Soc. 1992, 114, 18, 7303–7305Publication Date (Print):August 1, 1992Publication History Published online1 May 2002Published inissue 1 August 1992https://doi.org/10.1021/ja00044a057RIGHTS & PERMISSIONSArticle Views414Altmetric-Citations124LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (429 KB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Single-layer graphene nanosheets with controlled grafting of polymer chains (2010)

Single-layer graphene nanosheets (SLGNs) are prepared by reduction of well-exfoliated graphite oxide aided by a surfactant (sodium dodecylbenzene sulfonate, SDBS). Grafting density and polystyrene (PS) chain lengths are controlled by modulating the concentrations of diazonium compound and monomer during the grafting reaction of the initiator and the succeeding atomic transfer radical polymerization (ATRP). Atomic force microscopy (AFM), X-ray diffraction (XRD), Raman spectra and transmission electron microscopy (TEM) are used to confirm the single-layer structure of graphene sheets, covalent bonding at the interface, and distribution uniformity of grafting PS chains at the SLGN surface. Thermogravimetric analysis (TGA) is performed to assess the control of grafting density and chain length. PS chains grafted on the SLGN surface exhibited remarkably confined relaxation behavior. An increase in the glass transition temperature (Tg) of up to 18 °C is observed for high grafting density, low molecular weight polymer-grafted graphene samples. The low grafting density, high molecular weight sample shows an increase in Tg of ∼9 °C, which is attributed to superior heat conduction efficiency. The measured thermal conductivity for the PS composite film with 2.0 wt% SLGNs increase by a factor of 2.6 compared to that of the pure PS.
Calculation of Rayleigh backscattering noise in fiber-optic gyroscopes (1985)

Rayleigh backscattering noise in fiber-optic gyroscopes is calculated by taking into account the Rayleigh backscattered wave phases. The property of a low-coherence light wave is introduced by means of the analytic signal of the light source. The noise-equivalent rotation rate of Rayleigh backscattering is shown to be a factor of 2|1−2K| smaller than the value previously reported, where K is the power-splitting ratio including the effect of fiber loss in the sensing loop.
Zone‐specific gene expression patterns in articular cartilage (2012)

These differences in cartilage zonal gene expression identify new markers and pathways that govern the unique differentiation status of chondrocyte subpopulations.
Electron Permeable Self-Assembled Monolayers of Dithiolated Aromatic Scaffolds on Gold for Biosensor Applications (2008)

Self-assembled monolayers (SAMs) of thiolated compounds are formed by the spontaneous chemisorption of thiolate groups on metal surfaces. In biosensors, they are most commonly used to covalently immobilize a biorecognition molecule onto the surface of the transducer, thus offering the possibility of controlling the orientation, distribution, and spacing of the sensing element while reducing nonspecific interactions. In this paper, self-assembled monolayers of dithiolated derivatives of 3,5-dihydroxybenzyl alcohol containing carboxyl and hydroxyl end groups have been prepared on gold surfaces and characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Impedance measurements revealed that SAM formation is essentially completed after 3-5 h of exposure by observing the successive blocking of the faradic response of ferricyanide anion due to the adsorption of the dithiol molecules. The surface coverage of these molecules, estimated by reductive desorption experiments, was in the range of (1.1-2.8) x 10-10 mol/cm2. To demonstrate the potential of the dithiol SAM, a model system for detection of a tumor marker, prostate-specific antigen (PSA), was developed. The carboxyl groups of the SAM were succinimide-activated, and an anti-PSA antibody was covalently immobilized via amide bonds. The modified SAM was used for the label-free detection of prostate-specific antigen using EIS with a detection limit of 9 ng/mL. The results described here demonstrate that this kind of dithiol-modified SAM can be used as supports in electrochemical biosensors and the results are explained in terms of the structural features of these dithiols.
Retinotopic organization of early visual spatial attention effects as revealed by PET and ERPs (1997)

Cerebral blood flow PET scans and high-density event-related potentials (ERPs) were recorded (separate sessions) while subjects viewed rapidly-presented, lower-visual-field, bilateral stimuli. Active attention to a designated side of the stimuli (relative to passive-viewing conditions) resulted in an enhanced ERP positivity (P1 effect) from 80-150 msec over occipital scalp areas contralateral to the direction of attention. In PET scans, active attention vs. passive showed strong activation in the contralateral dorsal occipital cortex, thus following the retinotopic organization of the early extrastriate visual sensory areas, with some weaker activation in the contralateral fusiform. Dipole modeling seeded by the dorsal occipital PET foci yielded an excellent fit for the P1 attention effect. In contrast, dipoles constrained to the fusiform foci fit the P1 effect poorly, and, when the location constraints were released, moved upward to the dorsal occipital locations during iterative dipole fitting. These results argue that the early ERP P1 attention effects for lower-visual-field stimuli arise mainly from these dorsal occipital areas and thus also follow the retinotopic organization of the visual sensory input pathways. These combined PET/ERP data therefore provide strong evidence that sustained visual spatial attention results in a preset, top-down biasing of the early sensory input channels in a retinotopically organized way.
Development of a Rubella Virus Vaccine Expression Vector: Use of a Picornavirus Internal Ribosome Entry Site Increases Stability of Expression (2000)

Rubella virus (RUB) is a small plus-strand RNA virus classified in the Rubivirus genus of the family Togaviridae. Live, attenuated RUB vaccines have been successfully used in vaccination programs for over 25 years, making RUB an attractive vaccine vector. In this study, such a vector was constructed using a recently developed RUB infectious cDNA clone (Robo). Using a standard strategy employed to produce expression and vaccine vectors with other togaviruses, the subgenomic promoter was duplicated to produce a recombinant construct (termed dsRobo) that expressed reporter genes such as chloramphenicol acetyltransferase and green fluorescent protein (GFP) under control of the second subgenomic promoter. However, expression of the reporter genes, as exemplified by GFP expression by dsRobo/GFP virus, was unstable during passaging, apparently due to homologous recombination between the subgenomic promoters leading to deletion of the GFP gene. To improve the stability of the vector, the internal ribosome entry site (IRES) of a picornavirus, encephalomyocarditis virus, was used instead of the second subgenomic promoter to eliminate homology. Construction was initiated by first replacing the subgenomic promoter in the parent Robo infectious clone with the IRES. Surprisingly, viable virus resulted; this virus did not synthesize a subgenomic RNA. The subgenomic promoter was then reintroduced in an orientation such that a single subgenomic RNA was produced, GFP was the initial gene on this RNA, while the RUB structural protein open reading frame was downstream and under control of the IRES element. GFP expression by this vector was significantly improved in comparison to dsRobo/GFP. This strategy should be applicable to increase the stability of other togavirus vectors.
Recovery of Potassium from K-Feldspar by Thermal Decomposition with Flue Gas Desulfurization Gypsum and CaCO3: Analysis of Mechanism and Kinetics (2016)

The thermal decomposition mechanism of K-feldspar with industrial waste of FGD gypsum to produce soluble potassium (K) salt was investigated. Effects of the reaction temperature and the amount of reagents used on the recovery of K were studied. The results showed that increasing the reaction temperature and mass ratio of CaCO3/KAlSi3O8 and CaSO4/KAlSi3O8 was beneficial to the decomposition of K-feldspar. The recovery ratio of K was higher than 90% with the mass ratio of KAlSi3O8:CaSO4:CaCO3 = 1:1:3 at 1373 K for 40 min, and the product K2SO4 with a purity of 91.3% was obtained. A crystal structure disintegration mechanism for KAlSi3O8 was proposed on the basis of the characterization of phase transformation sequences by XRD, FTIR, and SEM/EDS. It was found that two product layers formed successively during the KAlSi3O8 decomposition process. K was enriched in the outer product layer, and the decomposition rate was controlled by Ca diffusion through the inner one. Based on the experimental results, a kinetics model of K-feldspar decomposition was established using the Crank-Ginstling-Brounshtein equation, and the apparent activation energy was determined.
Differential binding of plasminogen to crosslinked and noncrosslinked fibrins: its significance in hemostatic defect in factor XIII deficiency (1984)

In spontaneous fibrinolysis of an alpha 2-plasmin inhibitor-deficient plasma clot or tissue-type plasminogen activator-induced fibrinolysis in a purified system without alpha 2-plasmin inhibitor, the lysis was faster when factor XIII-mediated crosslinking of fibrin to fibrin did not occur. During the initial period, the binding of plasminogen to fibrin steadily increased with incubation time. The initial level and subsequent increase of the binding, which may be critical for the subsequent development of fibrinolysis, were more remarkable when fibrin was not crosslinked. The amount of glu- or lys-plasminogen bound to noncrosslinked fibrin was around 4 or 1.5 times larger than the amount of the respective plasminogen bound to crosslinked fibrin. Plasmin was also found to be bound to noncrosslinked fibrin twice as much as the amount bound to crosslinked fibrin. Structural changes induced by crosslinking of fibrin alpha-chain may reduce either the affinity or the number of available complementary sites to lysine binding sites of plasmin(ogen), thereby decreasing the binding of plasmin(ogen) to fibrin. These results suggest that an increased affinity of noncrosslinked fibrin for plasmin(ogen) is contributory to the accelerated fibrinolysis observed in factor XIII deficiency, in addition to an absence of crosslinking of alpha 2-plasmin inhibitor to fibrin.
Analysis of Overall Survival in Patients with Nonmetastatic Castration-Resistant Prostate Cancer Treated with Vaccine, Nilutamide, and Combination Therapy (2008)

These data indicate that patients with nonmetastatic castration-resistant prostate cancer (D0.5) who receive vaccine before second-line hormone therapy may potentially result in improved survival compared with patients who received hormone therapy and then vaccine. These data also suggest that patients with more indolent disease may derive greater clinical benefit from vaccine alone or vaccine before second-line hormone therapy compared with hormone therapy alone or hormone therapy followed by vaccine. These findings have potential implications for both the design and endpoint analysis of larger vaccine combination therapy trials.
Replication of Genetic Information with Self‐Encoded Replicase in Liposomes (2008)

In all living systems, the genome is replicated by proteins that are encoded within the genome itself. This universal reaction is essential to allow the system to evolve. Here, we have constructed a simplified system involving encapsulated macromolecules termed a "self-encoding system", in which the genetic information is replicated by self-encoded replicase in liposomes. That is, the universal reaction was reconstituted within a microcompartment bound by a lipid bilayer. The system was assembled by using one template RNA sequence as the information molecule and an in vitro translation system reconstituted from purified translation factors as the machinery for decoding the information. In this system, the catalytic subunit of Qbeta replicase is synthesized from the template RNA that encodes the protein. The replicase then replicates the template RNA that was used for its production. This in-liposome self-encoding system is one of the simplest such systems available; it consists of only 144 gene products, while the information and the function for its replication are encoded on different molecules and are compartmentalized into the microenvironment for evolvability.
I. Segregation of Active Constituents from Tablet Formulations During Grinding: Theoretical Considerations (1982)

AbstractThe preparatory step in the analysis of active drugs in tablet dosage forms has generally consisted of the grinding or milling of a given number of the tablets into a uniformly composited powder. Since the advent of content uniformity testing (individual tablet analysis) where the compositing step is bypassed, numerous reports have cited the large percentage difference in results observed between the average composited assay value and the average assay value for the individual tablets.This paper describes some of the factors responsible for these observed differences, as well as some of the causative events that develop during tablet grinding. Certain active constituents become separated from other tablet components during grinding because of differences in the size, shape, density, surface roughness, and resilience of their particles. At particle sizes of less than approximately 400 µm additional forces and mechanisms, namely Van der Waals forces, electrostatic forces, adsorption, and valency forces, become significant.
Separation of Skin Reactive Intestinal Cancer Antigen from the Carcinoembryonic Antigen of Gold (1972)

Soluble fractions of human intestinal cancer and fetal intestinal cell membranes produced delayed hypersensitivity reactions in patients with intestinal cancer. These soluble fractions and perchloric acid extracts of intestinal cancer cells were fractionated by polacrylamide-gel electrophoresis. The Gold carcinoembryonic antigen was found in a region of the gels different from that of the skin reactive antigen.
A Power-Control Scheme With Constant Switching Frequency in Class-D Inverter for Induction-Heating Jar Application (2007)

In this paper, a simple power-control scheme for a constant-frequency class-D inverter with variable duty cycle at constant frequency is proposed. It is more suitable and acceptable for high-frequency induction-heating (IH) jar applications. The proposed control scheme has the advantages of not only wide power-regulation range but also ease of output-power control. In addition, it can achieve stable and efficient zero-voltage switching in the whole load range. The control principles of the proposed method are described in detail, and the validity is verified through the simulated and experimental results on the 42.8-kHz insulated gate bipolar transistor for an IH rated on 1.6 kW with constant-frequency variable power