gebhart/patrepeval huggingface dataset:
Automated Discovery and Maintenance of Enterprise Topology Graphs (2013)

Enterprise Topology Graphs (ETGs) represent a snapshot of the complete enterprise IT, including all its applications, processes, services, components, and their dependencies. In the past, ETGs have been applied in analysis, optimization, and adaptation of enterprise IT. But how to discover and maintain a complete, accurate, fresh, and fine-grained Enterprise Topology Graph? Existing approaches either do not provide enough technical details or do not cover the complete scope of Enterprise Topology Graphs. Although existing tools are able to discover valuable information, there is no means for seamless integration. This paper proposes a plug in-based approach and extensible framework for automated discovery and maintenance of Enterprise Topology Graphs. The approach is able to integrate various kinds of tools and techniques into a unified model. We implemented the proposed approach in a prototype and applied it to different scenarios. Due to the vital role of discovery plugins in our approach, we support plug in development with a systematic testing method and discuss the lessons we learned. The results presented in this paper enable new ways of enterprise IT optimization, analysis, and adaptation. Furthermore, they unlock the full potential of past research, which previously required manual modeling of ETGs.
Cloning sequencing of Lol pI, the major allergenic protein of rye‐grass pollen (1991)

We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.
Electron transfer reactions in the soluble methane monooxygenase of Methylococcus capsulatus (Bath) (1985)

Aerobic stopped-flow experiments have confirmed that component C is the methane monooxygenase component responsible for interaction with NADH. Reduction of component C by NADH is not the rate-limiting step for component C in the methane monooxygenase reaction. Removal and reconstitution of the redox centres of component C suggest a correlation between the presence of the FAD and Fe2S2 redox centres and NADH: acceptor reductase activity and methane monooxygenase activity respectively, consistent with the order of electron flow: NADH----FAD----Fe2S2----component A. This order suggests that component C functions as a 2e-1/1e-1 transformase, splitting electron pairs from NADH for transfer to component A via the one-electron-carrying Fe2S2 centre. Electron transfer has been demonstrated between the reductase component, component C and the oxygenase component, component A, of the methane monooxygenase complex from Methylococcus capsulatus (Bath) by three separate methods. This intermolecular electron transfer step is not rate-determining for the methane monooxygenase reaction. Intermolecular electron transfer was independent of component B, the third component of the methane monooxygenase. Component B is required to switch the oxidase activity of component A to methane mono-oxygenase activity, suggesting that the role of component B is to couple substrate oxidation to electron transfer, via the methane monooxygenase components.
Developing rapid, point-of-care, multiplex detection for use in lateral flow devices (2005)

Immunoassays have been widely used in commercial, scientific and medical research for detection and quantification of analytes in complex mixtures. There is however a need for a point-of-care, multiplex diagnostic assays capable of providing rapid and quantitative measurements of analytes present in samples that are sufficiently simple to carry out without use of a laboratory or individuals trained in chemical analysis. We are developing a fluorescent lateral flow immunoassay platform to perform simultaneous, multiplexed detection of analytes in a complex fluid mixture along with instrumentation to optically quantitate the analytes in the sample. Our prototype imaging system is based on conventional 16-bit CCD optics, which enables the development of a rugged diagnostic instrument that can be further scaled down for point-of-care applications. We have compared protein microarrays with lateral flow assays (LFAs) to determine the sensitivity of each system for the measurement of distinct proteins in complex samples. We are pursuing the LFA platform such that it can easily be scaled to meet the requirements of any given screening application, and be implemented for use in a medical or surgical setting.
Discovery of Quinazolines as Histamine H4Receptor Inverse Agonists Using a Scaffold Hopping Approach (2008)

From a series of small fragments that was designed to probe the histamine H(4) receptor (H(4)R), we previously described quinoxaline-containing fragments that were grown into high affinity H(4)R ligands in a process that was guided by pharmacophore modeling. With a scaffold hopping exercise and using the same in silico models, we now report the identification and optimization of a series of quinazoline-containing H(4)R compounds. This approach led to the discovery of 6-chloro-N-(furan-3-ylmethyl)2-(4-methylpiperazin-1-yl)quinazolin-4-amine (VUF10499, 54) and 6-chloro-2-(4-methylpiperazin-1-yl)-N-(thiophen-2-ylmethyl)quinazolin-4-amine (VUF10497, 55) as potent human H(4)R inverse agonists (pK(i) = 8.12 and 7.57, respectively). Interestingly, both compounds also possess considerable affinity for the human histamine H(1) receptor (H(1)R) and therefore represent a novel class of dual action H(1)R/H(4)R ligands, a profile that potentially leads to added therapeutic benefit. Compounds from this novel series of quinazolines are antagonists at the rat H(4)R and were found to possess anti-inflammatory properties in vivo in the rat.
Clinical Pharmacology of Human Insulin (1993)

Nowadays, human insulin is used daily by millions of diabetic patients. The biological effect of human insulin is comparable to that of porcine insulin. However, after subcutaneous injection, pharmacological and clinical studies showed pharmacokinetic and pharmacodynamic differences between human and animal insulins. Human insulin tends to have faster absorption and shorter duration of action compared with animal insulin. These differences are more pronounced and can be of clinical relevance with intermediate- and long-acting insulin preparations. Optimal metabolic control can be achieved with either human or highly purified animal insulin preparations, provided appropriate insulin replacement strategies are used.
Bacteremia Due to Mycobacterium avium-intracellulare in the Acquired Immunodeficiency Syndrome (1983)

The presence of Mycobacterium avium-intracellulare has frequently been demonstrated in tissue specimens from patients with the acquired immunodeficiency syndrome. The importance of this mycobacterium as a cause of constitutional symptoms and organ dysfunction has been unclear, however, because of the sparse inflammatory response evoked and the frequent concurrence of other pathogenic organisms. We detected M. avium-intracellulare in blood samples from eight patients with the acquired immunodeficiency syndrome, seven of whom had a previously recognized M. avium-intracellulare infection. Blood cultures were positive on 1 to 14 occasions over 135 days using the Dupont isolator system or the Bactec 12B medium system. Cultures were positive within 14 to 51 or 7 to 14 days with the respective techniques. The ability to rapidly isolate M. avium-intracellulare from blood may provide a useful diagnostic technique for detecting disseminated mycobacterial disease in patients with the acquired immunodeficiency syndrome as well as in other patient populations. This technique may also be useful for assessing the efficacy of drug therapy.
The marks, mechanisms and memory of epigenetic states in mammals (2001)

It is well recognized that there is a surprising degree of phenotypic variation among genetically identical individuals, even when the environmental influences, in the strict sense of the word, are identical. Genetic textbooks acknowledge this fact and use different terms, such as ‘intangible variation’ or ‘developmental noise’, to describe it. We believe that this intangible variation results from the stochastic establishment of epigenetic modifications to the DNA nucleotide sequence. These modifications, which may involve cytosine methylation and chromatin remodelling, result in alterations in gene expression which, in turn, affects the phenotype of the organism. Recent evidence, from our work and that of others in mice, suggests that these epigenetic modifications, which in the past were thought to be cleared and reset on passage through the germline, may sometimes be inherited to the next generation. This is termed epigenetic inheritance, and while this process has been well recognized in plants, the recent findings in mice force us to consider the implications of this type of inheritance in mammals. At this stage we do not know how extensive this phenomenon is in humans, but it may well turn out to be the explanation for some diseases which appear to be sporadic or show only weak genetic linkage.
Stereoselective Nucleophilic Addition Reactions of Reactive Pseudopeptides (1992)

D,L-Pseudopeptides of type 1 undergo 1,4-additions with tBu2CuLi/Me3SiCl with high stereoselectivity, whereas reactions of the corresponding L,L diastereomers are substantially less diastereoselective. The L,L,L and D,L,L analogues also react with different stereoselectivities. Strict chelation control plays a role in the Grignard-like reaction of L,L peptide aldehydes like 2 with (CH3)2CuLi.
Near-field subwavelength micropattern generation: Pipette guided argon fluoride excimer laser microfabrication (1992)

Near-field optical methods are used, together with the ability of an argon fluoride excimer laser to remove a wide variety of materials without the deposition of detectable heat, to produce structures with nanometer dimensionalities. In this new method of lithography, a hollow glass micropipette is used to guide the 193 nm light of the excimer laser to the surface to be ablated. With such micropipettes, patterns have been produced on photoresists with linewidths that are as small as 70 nm. By investigating the dimensionality of the structures drawn on the photoresist and on the substrate on which the photoresist was deposited, it appears that nonlinear characteristics of the ablation process may allow the near-field dimensionalities to be maintained at distances that are relatively remote from the tip of the pipette aperture.
[Immunologic foundation of enzyme therapy of patients with orchiepididymitis]. (1990)

Twenty eight patients aged 18 to 40 years with urogenital infections complicated by orchiepididymitis were examined immunologically. The use of specific monoclonal antibodies OKT-4+ and OKT-8 revealed lower contents of T-helper cells (Thc) (23.33 +/- 9.7%) and higher contents of T-suppressor cells (Tsc) (41.17 +/- 13.41%) in them as compared to those in healthy volunteers (18 persons) and patients with noncomplicated urogenital infections (19 persons). There was a significant decrease in the immunoregulatory index (IRI): Thc/Tsc (0.68 +/- 0.33) reached in some patients the ratio observed in persons infected with the HIV (0.6 +/- 0.2) and an increase in the contents of circulating immune complexes (CIC) (28.5 +/- 2.0 arbitrary units against the normal of 10.0 +/- 0.7). Enzyme therapy including the use of chymotrypsin and trypsin manufactured in the USSR in doses of 10 mg each administered intramuscularly once a day or in doses of 5 mg each administered intramuscularly twice a day for 10 days promoted normalization of the impaired immunity indices (Thc 44.0 +/- 10.51%, Tsc 31.5 +/- 11.26%, IRI 1.53 +/- 0.33 and CIC 12.1 +/- 0.74 arbitrary units). Therefore, the enzymes had an immunomodulating effect. It is likely that they will be useful in treatment of infectious inflammatory processes accompanied by analogous immune disorders which makes the enzyme immunocorrection more applicable in treatment of various pathological processes.
'Spicy cup-of-tea counselling': the therapist in the client's home environment (2008)

The client's home environment is a rich source of information about their lives, culture, beliefs, psyches, strengths, relationships and emotional worlds. The reluctance in the therapeutic community to provide counselling to clients in their home environment reflects concerns about personal safety and the lack of a therapeutic boundaries. But are these concerns valid? Le Hoang presents an alternative position in the face of an increasing trend in our profession toward defensive and sanitised practice. A case study illustrates the therapeutic use of the client's environment in the provision of counselling. While home visit therapy suits certain client groups, and not others, Hoang explores what stops us as a profession from welcoming the client's home environment and making the best use of it in our work. She asks if 'individualism' has killed the capacity of people to be in an intimate space with each other, and have fears of being judged as 'unprofessional' de-skilled counsellors when it comes to embracing home visit counselling? (editor abstract)
<title>Structural effects of DNQ-PAC backbone on resist lithographic properties</title> (1991)

Model backbones without hydroxyl groups and fully esterified diazonaphthoquinone PACs were studied to identify critical structural parameters for dissolution inhibition in conventional diazonaphthoquinone/novolac photoresist systems. Hydrophobicity, presence of a site interactive with novolac, and proximaity of DNQ groups were identified as critical parameters. In general, the inhibiting ability of model compounds or PACs were found to be correlated with their retention time on reverse phase HPLC, a measure of hydrophobicity. Evidence is shown there to support the suggestion that the DNQ group provides a strong hydrogen bonding site to enhance efficiency of inhibition. PACs having DNQ groups in close proximity had lower inhibition than PACs with DNQs far apart.
Potency of influenza vaccines: mouse protection experiments in correlation to field studies in man. (1975)

The seroconversion rates have been studied following vaccination of human volunteers with two commercial influenza vaccines. Vaccine A did not give a significant increase of hemagglutination-inhibition titers. Vaccine B, on the other hand, raised the titers 2- to 8- fold, depending on the pretiters of the individuals. The potency of the same vaccines has been tested using mouse protection experiments: vaccine B gave significantly better protection rates, as measured by survival as well as by reduction of lung lesions. These results give additional evidence that the use of mouse protection experiments for the evaluation of different influenza vaccines is meaningful.
Effects of selenium on 7,12-dimethylbenz(a)anthracene-induced mammary carcinogenesis and DNA adduct formation. (1985)

The purpose of the present investigation was to determine the effects of dietary selenium deficiency or excess on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary neoplasia in rats and to delineate whether selenium-mediated modification of mammary carcinogenesis was associated with changes in carcinogen:DNA adduct formation and activities of liver microsomal enzymes that are involved in xenobiotic metabolism. Female Sprague-Dawley rats were divided into three groups from weaning and were maintained on one of three synthetic diets designated as follows: selenium deficient (less than 0.02 ppm); selenium adequate (0.2 ppm); or selenium excess (2.5 ppm). For the DMBA binding and DNA adduct studies, rats were given a dose of [3H]DMBA p.o. after 1 month on their respective diets. Results from the liver and the mammary gland indicated that neither selenium deficiency nor excess had any significant effect on the binding levels, which were calculated on the basis of total radioactivity isolated with the purified DNA. Furthermore, it was found that dietary selenium intake did not seem to affect quantitatively or qualitatively the formation of DMBA:DNA adducts in the liver. Similarly, in a parallel group of rats that did not receive DMBA, the activities of aniline hydroxylase, aminopyrine N-demethylase, and cytochrome c reductase were not significantly altered by dietary selenium levels. Concurrent with the above experiments, the effect of dietary selenium intake on carcinogenesis was also monitored. Results of this experiment indicated that selenium deficiency enhanced mammary carcinogenesis only when this nutritional condition was maintained in the postinitiation phase. Likewise, an excess of selenium intake inhibited neoplastic development only when this regimen was continued after DMBA administration. In either case, deficient or excess selenium at the time of carcinogenic insult failed to produce a significant effect on subsequent tumor yield, if selenium intake was returned to normal during the proliferative phase of tumor growth. Based on the results of these studies, it is suggested that selenium-mediated modification of mammary tumorigenesis is not exerted via alterations in carcinogenic initiation (i.e., metabolism or DNA adduct formation).
Inductive plethysmography components analysis and improved non-invasive postoperative apnoea monitoring (1999)

Twenty-nine patients were monitored overnight for breathing distress patterns during postoperative analgesia. Nasal flow apnoea monitoring and pulse oximetry data were recorded at 50 Hz. Respiratory inductive plethysmography (RIP) tracked tidal volume (TV) thoracoabdominal motion, and supplemented the flow monitoring by identifying detected apnoea type. TVs were computed from linear combinations of the RIP signals, but calibrations showed that multiple regression approaches with fitting errors <1% had highly variable coefficients and estimate precisions. Simple least squares theory showed that unstable parameter calculation and coefficient variation with signal conditions were inherent in RIP calibration models. Principal components (PC) methods were well suited to mitigating these problems because the RIP signals were highly correlated. The two PCs tracked the relative changes in TVs and indicated the degree of signal asynchrony, enabling improved uncalibrated monitoring. For accurately measuring RIP phase differences, the cross-correlation function was calculated. A simple version of PC analysis is developed, avoiding matrices, to help clarify how RIP calibration problems can be addressed. The methods are illustrated for calibration in normal breathing, and for postoperative monitoring during Cheyne-Stokes breathing. Sum and difference combinations of the RIP signals could discriminate central from obstructive apnoeas to help improve flow monitoring efficacy on-line.
Implications of Fracturing Pressure Data Recorded during a Horizontal Completion on Stage Spacing Design (2012)

Abstract Horizontal completions have changed considerably in the last few years in an effort to substantially improve the drainage of shale gas reservoirs. The spacing of fracture stages and perforation clusters are among the most crucial completion decisions that impact well productivity and EUR. Yet, the decision regarding stage spacing is rarely guided by an engineering process, as it remains a challenge to tie production performance and completion design. In this paper, we offer some insight on the impact of fracture spacing on the propagation direction of multiple transverse fractures, and consequently the expected performance of the horizontal well. Stress-shadow effects, related to the mechanical interference induced by a proppant-filled fracture, can cause fractures initiated from a horizontal well to deviate toward or away from previous fractures. A three-dimensional geomechanical model of the combined stress interference from multiple transverse fractures has been applied to typical wells in three shale gas reservoirs: Bakken, Barnett and Eagle Ford. The existence of an optimum spacing is demonstrated, where fracture stages remain transverse even when subject to stress-shadow effects. Below the optimum spacing, induced fractures may intersect previous fractures, and re-stimulate previously fractured regions of the reservoir, while leaving undrained portions of the reservoir un-stimulated. Such behavior is highly dependent on the mechanical properties of the shale, in particular the Young’s modulus. Our modeling results suggest that the net fracturing pressure data measured in the field reflects the propagation direction of the fractures induced from the horizontal wellbore. A monotonic increase in net pressure, going from one stage to another, would indicate transverse fracture propagation during all stages. On the other hand, an up-and-down trend in the net pressure data is an indication that the mechanical stress interference is causing the later stage fractures to intersect fractures from previous stages. The net pressure data can, therefore, be used to investigate fracture-to-fracture interference and can be used to optimize the spacing of fracture stages in horizontal completions.
Transduction of Tumor-Specific T Cells with CXCR2 Chemokine Receptor Improves Migration to Tumor and Antitumor Immune Responses (2010)

These results implicate that the introduction of the CXCR2 gene into tumor-specific T cells can enhance their localization to tumors and improve antitumor immune responses. This strategy may ultimately enable personalization of cancer therapies based on chemokine expression by tumors.
Providing dynamic update in an operating system (2005)

Dynamic update is a mechanism that allows software updates and patches to be applied to a running system without loss of service or down-time. Operating systems would benefit from dynamic update, but place unique demands on any implementation of such features. These demands stem from the event-driven nature of operating systems, from their restricted run-time execution environment, and from their role in simultaneously servicing multiple clients. We have implemented a dynamic update mechanism in the K42 research operating system, and tested it using previous modifications of the system by kernel developers. Our system supports updates changing both kernel code and data structures. In this paper we identify requirements needed to provide a dynamically updatable operating system, describe our implementation, and present our experiences in designing and using the dynamic update mechanism. We also discuss its applicability to other operating systems.