gebhart/patrepeval huggingface dataset:
The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. (1990)

The alpha subunit of the FcERI binds IgE with high affinity. Previous studies have demonstrated that alpha subunit expression requires the presence of beta and/or gamma subunits, and it is not known how these two subunits contribute to the ability of the alpha subunit to bind IgE. In this report, we describe the expression and characterization of a human chimeric alpha subunit. The data demonstrate that high affinity IgE binding does not require the presence of the beta and/or gamma subunits and that this activity is localized to the extracellular domain (residues 26-201) of the human alpha subunit. Permanent cell lines expressing the chimeric receptor were used to characterize the binding parameters of the alpha subunit. These cell lines provide a means of identifying therapeutic agents which may be effective in the treatment/management of allergic diseases.
Growth hormone therapy. (1999)

Growth hormone therapy with rhGH (recombinant human growth hormone) has been recommended for treatment of GH deficient short stature in children, repeated hypoglycemias in infancy and early childhood due to GH deficiency, short stature accompanying chronic renal failure prior to renal transplantation and Turner's syndrome. It is now increasingly recommended to adults with GH deficiency following pituitary tumour surgery or irradiation or idiopathic hypopituitarism. There are other indications for its use where evidence for protein catabolism is very strong such as burns injury. The end points of GH therapy in children include achievement of desirable adult height or a growth rate velocity of < 2.5 cm/year. In adults GH deficiency, GH therapy is intended for improvement of general well being, body composition and metabolic markers of GH function.
Towards a general location service for mobile environments (2002)

With the advent of mobile computing devices and cheap location sensing systems, location information has become an important resource both for mobile and 'desktop' users. We describe some key concepts a scalable ubiquitous location service should be based on. Firstly, we discuss the general problem of client-server computing in mobile environments. Secondly, we describe the theoretical and design framework for a general location service. We address the use of multiple sources of location information. Further, we describe location domains as a powerful framework for presenting and protecting location information.
Interleukin‐31 does not induce immediate itch in atopic dermatitis patients and healthy controls after skin challenge (2013)

IL-31 does not induce immediate itch responses in humans. The late onset of IL-31-induced itch supports the notion that IL-31 exerts its pruritic effect indirectly via keratinocytes and secondary mediators, rather than through its receptors on cutaneous nerves.
Cognitive fMRI and soluble telencephalin assessment in patients with localization-related epilepsy (2008)

Objectives - The use of telencephalin as a possible marker for altered cortical function as demonstrated by functional MRI was investigated in a pilot study with 16 patients with localization-related epilepsy and secondarily generalized seizures. Materials and methods - Functional MRI of verbal working memory performance (Sternberg paradigm) and self-regulatory control processes (Stroop paradigm) was used to examine cortical activation in 16 patients with localization-related epilepsy and secondarily generalized seizures. Additionally, blood serum concentrations of soluble telencephalin (marker for neuronal damage) were determined. Results - In three patients (one temporal and two frontal focus), telencephalin was detected. All three patients had lower functional MRI activation in the frontotemporal region (P = 0.04), but not in other regions (P > 0.35) compared with patients without detectable telencephalin. Additionally, an association of levetiracetam and frontotemporal activation was observed. Conclusions - These preliminary data in a heterogeneous group suggest an association between decreased frontotemporal activation on fMRI and both detectable telencephalin serum levels and levetiracetam use. Future longitudinal studies with larger patient groups are required to confirm these observations. It is hypothesized that altered local function of the frontotemporal cortex in localization-related epilepsy might be better predicted by the biochemical marker telencephalin than epilepsy characteristics such as seizure focus.
Calcium-binding affinity and calcium-enhanced activity of Clostridium thermocellum endoglucanase D (1990)

Clostridium thermocellum endoglucanase D (EC 3.2.1.4: EGD), which is encoded by the celD gene, was found to bind Ca2+ with an association constant of 2.03 x 10(6) M-1. Ca2+ stimulated the activity of EGD towards swollen Avicel by 2-fold. In the presence of Ca2+, the Kd of the enzyme towards p-nitrophenyl-beta-D-cellobioside and carboxymethylcellulose was decreased by 4-fold. Furthermore, Ca2+ increased the half-life of the enzyme at 75 degrees C from 13 to 47 min. Since the 3' sequence of celD encodes a duplicated region sharing similarities with the Ca2+-binding site of several Ca2+-binding proteins, a deleted clone was constructed and used to purify a truncated form of the enzyme which no longer contained the duplicated region. The truncated enzyme was very similar to EGD expressed from the intact gene with respect to activity, Ca2(+)-binding kinetics and Ca2+ effects on substrate binding and thermostability. Thus the latter parameters do not appear to be mediated through the duplicated conserved region.
Ingestion of Diet Soda Before a Glucose Load Augments Glucagon-Like Peptide-1 Secretion (2009)

Artificial sweeteners synergize with glucose to enhance GLP-1 release in humans. This increase in GLP-1 secretion may be mediated via stimulation of sweet-taste receptors on L-cells by artificial sweetener.
ChemInform Abstract: BE‐23372M, a Novel Protein Tyrosine Kinase Inhibitor. Part 3. Synthesis. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The Role of PD-1 Ligand in Immune Evasion by Breast Cancer (2005)

Abstract : The PD-1/PD-1 Ligand pathway inhibits T cell activation. We demonstrated expression of PD-L1 by immunohistochemistry on breast tumor cells in situ but PD-L1 was not expressed on normal breast epithelium. PD-L1 expression provides a mechanism whereby tumor cells can inhibit immune responses. We also demonstrated expression of PD-L1 in breast cancer cell lines. MDA231 has the highest PD-L1 expression among the cell lines tested. SKBR3, another breast cancer cell line, has the most PD-L2 expression. PD-L1 expression on MDA231 cells can be up-regulated by estrogen up to 50 microg/ml but there was no change in PD-L2 expression. We identified anti-PD-ligand antibodies with non-overlapping epitopes and developed an ELISA for detecting soluble PD-L1 in serum. In the small number of samples analyzed, there was no difference in soluble serum levels of PD-L1 between normal controls and breast cancer samples. Blockade of the PD-1/PD-1 Ligand pathway upregulated CD4(+) and CD8(+) allo-responses, in which either dendritic cells or MDA231 breast cancer cells were used as allo-stimulators. Blockade of the PD-1/PD-1 Ligand pathway also enhanced CD8 T cell IFN-gamma production and an increase in CD8 T cell number in an allo-response to MDA231 cells.
ChemInform Abstract: Synthesis of Carotenoids (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
sIL‐6R: more than an agonist? (2007)

On target cells, interleukin-6 (IL-6) interacts with its receptor complex consisting of the membrane-bound IL-6 receptor (IL-6R) and the signal transducing protein gp130. IL-6R can exist as a soluble protein (sIL-6R), which binds the ligand IL-6. This soluble complex can bind to gp130 on cells that lack the membrane-bound IL-6R and initiate signaling. This process is named transsignaling. The significance of transsignaling via sIL-6R is underlined by different publications and exceeds very probably the significance of the membrane-bound IL-6R. It is the general assumption that sIL-6R acts as an agonist in combination with IL-6 resulting in an enhancement of the IL-6 effects. In this article, we suppose 'non-agonistic' properties. There are several publications that give reasons to speculate that sIL-6R (a) has IL-6-antagonistic effects, (b) has orphan properties and (c) interacts with yet unknown binding partners different from IL-6. Knowledge about additional properties of sIL-6R will enlarge the biologic understanding of this molecule and might give an explanation for the sometimes contrasting effects of the cytokine IL-6.
Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein. (1986)

The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding alpha-melanocyte-stimulating hormone (alpha-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related alpha-MSH hybrid protein in which the diphtheria toxin receptor-binding domain has been replaced with alpha-MSH sequences. The chimeric toxin has been partially purified from periplasmic extracts of recombinant Escherichia coli K-12 and has been found to be selectively toxic for alpha-MSH receptor-positive human malignant melanoma NEL-M1 cells in vitro.
Differential regulation of chemokine receptors during dendritic cell maturation: a model for their trafficking properties. (1998)

Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present Ag. CC chemokines induce chemotactic and transendothelial migration of immature DC, in vitro. Maturation of DC by CD40L, or by LPS, IL-1, and TNF, induces down-regulation of the two main CC chemokine receptors expressed by these cells, CCR1 and CCRS, and abrogates chemotaxis to their ligands. Inhibition was rapid (<1 h) and included the unrelated agent FMLP. Concomitantly, the expression of CCR7 and the migration to its ligand EBI1 ligand chemokine (ELC)/macrophage inflammatory protein (MIP)-3beta, a chemokine expressed in lymphoid organs, were strongly up-regulated, though with slower kinetics (24-48 h). Rapid inhibition of responsiveness to chemoattractants present at sites of inflammation and immune reaction may be permissive for leaving peripheral tissues. Conversely, the slower acquisition of responsiveness to ELC/MIP-3beta may guide subsequent localization of DC in lymphoid organs.
The protein encoded by the Shewanella colwelliana melA gene is a p-hydroxyphenylpyruvate dioxygenase (1994)

The identity of the product of the melA gene from Shewanella colelliana with the enzyme p-hydroxyphenylpyruvic dioxygenase is shown. Cloning of the melA gene endowed Escherichia coli with the capacity to synthesize melanin-like pigments from L-tyrosine. E. coli contained transaminases that transforms L-tyrosine into p-hydroxyphenylpyruvate. This keto acid was detected in the cultures. On the other hand, E. coli containing melA was able to go further in the catabolic pathway, releasing a great amount of homogentisic acid. This acid can spontaneously polymerize giving the pigment. Furthermore, p-hydroxyphenylpyruvate dioxygenase activity was detected in this system. Analysis of the deduced amino acid sequence revealed a high homology with the p-hydroxyphenylpyruvate deoxygenase enzyme from different organisms.
Differential regulation of platelet‐derived growth factor stimulated migration and proliferation in osteoblastic cells (2004)

Osteoblastic migration and proliferation in response to growth factors are essential for skeletal development, bone remodeling, and fracture repair, as well as pathologic processes, such as metastasis. We studied migration in response to platelet-derived growth factor (PDGF, 10 ng/ml) in a wounding model. PDGF stimulated a twofold increase in migration of osteoblastic MC3T3-E1 cells and murine calvarial osteoblasts over 24-48 h. PDGF also stimulated a tenfold increase in 3H-thymidine (3H-TdR) incorporation in MC3T3-E1 cells. Migration and DNA replication, as measured by BrdU incorporation, could be stimulated in the same cell. Blocking DNA replication with aphidicolin did not reduce the distance migrated. To examine the role of mitogen-activated protein (MAP) kinases in migration and proliferation, we used specific inhibitors of p38 MAP kinase, extracellular signal regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). For these signaling studies, proliferation was measured by carboxyfluorescein diacetate succinimidyl ester (CFSE) using flow cytometry. Inhibition of the p38 MAP kinase pathway by SB203580 and SB202190 blocked PDGF-stimulated migration but had no effect on proliferation. Inhibition of the ERK pathway by PD98059 and U0126 inhibited proliferation but did not inhibit migration. Inhibition of JNK activity by SP600125 inhibited both migration and proliferation. Hence, the stimulation of migration and proliferation by PDGF occurred by both overlapping and independent pathways. The JNK pathway was involved in both migration and proliferation, whereas the p38 pathway was predominantly involved in migration and the ERK pathway predominantly involved in proliferation.
High-Concentration Preparation of Silver Nanowires: Restraining in Situ Nitric Acidic Etching by Steel-Assisted Polyol Method (2008)

A steel-assisted polyol method has been proposed for the preparation of Ag nanowires (AgNWs) at high AgNO3 concentrations of up to 0.5 M. The average diameter of products could be adjusted from approximately 100 to 300 nm by increasing the synthetic concentration. It was found that in situ nitric acidic etching was a fatal factor for the stability of the multiply twinned particles (MTPs), which were the seeds of AgNWs in the polyol synthesis system. The introduction of stainless steel could effectively consume the in situ generated nitric acid through a corrosion reaction between them, hence enabling the survival of MTPs for the consequent growth of AgNWs.
Electrical wound-healing assay for cells in vitro (2004)

Confluent cell monolayers in tissue culture are fragile and can easily be mechanically disrupted, often leaving an area devoid of cells. This opening in the cell sheet is then repopulated, because the cells on the fringe of the damage, which are no longer contact-inhibited, move into the available space. This mechanical disruption is often done deliberately in a "wound-healing" assay as a means to assess the migration of the cells. In such assays, a scrape is made in the cell layer followed by microscopy to monitor the advance of the cells into the wound. We have found that these types of assays can also be accomplished electrically. In this approach, cells growing on small electrodes and monitored by using electric cell-substrate impedance sensing are subjected to currents, resulting in severe electroporation and subsequent cell death. After this invasive treatment, the electrode's impedance is again monitored to chart the migration and ultimate healing of the wound. We report here that this procedure to study cell behavior is both highly reproducible, quantitative, and provides data similar to that acquired with traditional measurements.
Roles of the matrix and of the primary carbides in the general high temperature oxidation behaviour of cobalt-based superalloys. part3: the Co(10Ni,30Cr) matrix with tantalum carbides (2015)

Most of the cast cobalt-based superalloys contain interdendritic carbides to strengthened them against mechanical solicitations at high temperature. Among the most common carbides used in this field there are the chromium carbides the formation of which does not necessitate new carbide- former elemnts since chromium is always present for oxidation and corrosion resistance purpose. Such carbides may influence the general oxidation behaviour of the whole alloy. In order to specify their contribution a cobalt alloy containing 10 wt.Ni and 30 wt.%Cr as most of cobaltbased superalloys, and 0.5 wt.%C to obtain chromium carbides in the microstructure, was studied in oxidation for the whole same thermal cycle as applied to a Co-10Ni-30Cr ternary alloy preliminarily characterized in the first part of this study. Many parameters describing the oxidation during the heating, the isothermal stage and the cooling were compared between these two alloys. There was not systematically differences but it significantly appeared that the {chromium carbides}-containing alloy oxidized faster during the heating and the isothermal stage than the carbidesfree one. In contrast, if oxide spallation occurred for the studied alloy as previously also observed for the ternary alloy, this phenomenon was delayed later as is to say to lower temperatures.
Pivotal role of the renin/prorenin receptor in angiotensin II production and cellular responses to renin (2002)

Renin is an aspartyl protease essential for the control of blood pressure and was long suspected to have cellular receptors.We report the expression cloning of the human renin receptor complementary DNA encoding a 350-amino acid protein with a single transmembrane domain and no homology with any known membrane protein.Transfected cells stably expressing the receptor showed renin-and prorenin-specific binding.The binding of renin induced a fourfold increase of the catalytic efficiency of angiotensinogen conversion to angiotensin I and induced an intracellular signal with phosphorylation of serine and tyrosine residues associated to an activation of MAP kinases ERK1 and ERK2.High levels of the receptor mRNA are detected in the heart, brain, placenta, and lower levels in the kidney and liver.By confocal microscopy the receptor is localized in the mesangium of glomeruli and in the subendothelium of coronary and kidney artery, associated to smooth muscle cells and colocalized with renin.The renin receptor is the first described for an aspartyl protease.This discovery emphasizes the role of the cell surface in angiotensin II generation and opens new perspectives on the tissue renin-angiotensin system and on renin effects independent of angiotensin II.
Antagonistic Analogs of Luteinizing Hormone-Releasing Hormone Are Mast Cell Secretagogues (1986)

The histamine-releasing activity of luteinizing hormone-releasing hormone (LHRH) antagonistic analogs has been documented. Antagonists of LHRH elicited in vitro histamine release from mast cells obtained from previously unexposed rats. Intradermal injection of the antagonists caused increased local skin permeability. Anaphylactoid reactions followed subcutaneous injection of the antagonists and in some cases these edematous reactions were accompanied by increased serum histamine levels. These studies show that these small peptides can cause mast cell degranulation and suggest that the neuropeptide, LHRH, may have modulating effects on the immune system.
Electrospray ionization/magnetic sector mass spectrometry: calibration, resolution, and accurate mass measurements (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrospray ionization/magnetic sector mass spectrometry: calibration, resolution, and accurate mass measurementsRobert B. Cody, Jun. Tamura, and Brian D. MusselmanCite this: Anal. Chem. 1992, 64, 14, 1561–1570Publication Date (Print):July 15, 1992Publication History Published online1 May 2002Published inissue 15 July 1992https://pubs.acs.org/doi/10.1021/ac00038a012https://doi.org/10.1021/ac00038a012research-articleACS PublicationsRequest reuse permissionsArticle Views743Altmetric-Citations89LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Very‐long‐chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme (1997)

The Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene encodes a putative seed-specific condensing enzyme. It is the first of four enzyme activities that comprise the microsomal fatty acid elongase (FAE) involved in the biosynthesis of very-long-chain fatty acids (VLCFAs). FAE1 has been expressed in yeast and in tissues of Arabidopsis and tobacco, where significant quantities of VLCFAs are not found. The introduction of FAE1 alone in these systems is sufficient for the production of VLCFAs, for wherever FAE1 was expressed, VLCFAs accumulated. These results indicate that FAE1 is the rate-limiting enzyme for VLCFA biosynthesis in Arabidopsis seed, because introduction of extra copies of FAE1 resulted in higher levels of the VLCFAs. Furthermore, the condensing enzyme is the activity of the elongase that determines the acyl chain length of the VLCFAs produced. In contrast, it appears that the other three enzyme activities of the elongase are found ubiquitously throughout the plant, are not rate-limiting and play no role in the control of VLCFA synthesis. The ability of yeast containing FAE1 to synthesize VLCFAs suggests that the expression and the acyl chain length specificity of the condensing enzyme, along with the apparent broad specificities of the other three FAE activities, may be a universal eukaryotic mechanism for regulating the amounts and acyl chain length of VLCFAs synthesized.