gebhart/patrepeval huggingface dataset:
Engineered rRNA Enhances the Efficiency of Selenocysteine Incorporation during Translation (2012)

We developed a new genetic selection approach to screen for mutations that can alter the efficiency of selenocysteine incorporation. We identified mutations in 16S rRNA that increase or decrease the efficiency of selenocysteine incorporation in Escherichia coli without influencing the efficiency or fidelity of canonical translation. Engineered ribosomes with improved selenocysteine incorporation provide valuable tools for synthetic biology and biotechnology.
<title>Optical and thermal evaluation of transparent materials and surfaces by FTIR and integrating spheres</title> (1992)

Integrating sphere measurements are presented in the wavelength range from 0.35 micrometers to 18 micrometers . The spheres are used with a rapid scan FTIR Bruker IFS66. The design of the sphere allows the spectral reflectance and transmittance to be determined, both as normal hemispherical and as normal diffuse values. The excellent resolution and reproducibility is demonstrated by noise spectra and some measurements. The easy sample handling makes this instrument a versatile tool for the development of new materials as well as for aging investigations.
Mannich bases of 4-phenyl-3-buten-2-one. A new class of antiherpes agent (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMannich bases of 4-phenyl-3-buten-2-one. A new class of antiherpes agentM. L. Edwards, H. W. Ritter, D. M. Stemerick, and K. T. StewartCite this: J. Med. Chem. 1983, 26, 3, 431–436Publication Date (Print):March 1, 1983Publication History Published online1 May 2002Published inissue 1 March 1983https://pubs.acs.org/doi/10.1021/jm00357a020https://doi.org/10.1021/jm00357a020research-articleACS PublicationsRequest reuse permissionsArticle Views573Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Intracellular dynamics of Smad‐mediated TGFβ signaling (2003)

The transforming growth factor-beta (TGFbeta) family represents a class of signaling molecules that plays a central role in morphogenesis, growth, and cell differentiation during normal embryonic development. Members of this growth factor family are particularly vital to development of the mammalian secondary palate where they regulate palate mesenchymal cell proliferation and extracellular matrix synthesis. Such regulation is particularly critical since perturbation of either cellular process results in a cleft of the palate. While the cellular and phenotypic effects of TGFbeta on embryonic craniofacial tissue have been extensively catalogued, the specific genes that function as downstream mediators of TGFbeta action in the embryo during palatal ontogenesis are poorly defined. Embryonic palatal tissue in vivo and murine embryonic palate mesenchymal (MEPM) cells in vitro secrete and respond to TGFbeta. In the current study, elements of the Smad component of the TGFbeta intracellular signaling system were identified and characterized in cells of the embryonic palate and functional activation of the Smad pathway by TGFbeta1, TGFbeta2, and TGFbeta3 was demonstrated. TGFbeta-initiated Smad signaling in cells of the embryonic palate was found to result in: (1) phosphorylation of Smad 2; (2) nuclear translocation of the Smads 2, 3, and 4 protein complex; (3) binding of Smads 3 and 4 to a consensus Smad binding element (SBE) oligonucleotide; (4) transactivation of transfected reporter constructs, containing TGFbeta-inducible Smad response elements; and (4) increased expression of gelatinases A and B (endogenous genes containing Smad response elements) whose expression is critical to matrix remodeling during palatal ontogenesis. Collectively, these data point to the presence of a functional Smad-mediated TGFbeta signaling system in cells of the developing murine palate.
Preclinical targeting of human acute myeloid leukemia and myeloablation using chimeric antigen receptor–modified T cells (2014)

Many patients with acute myeloid leukemia (AML) are incurable with chemotherapy and may benefit from novel approaches. One such approach involves the transfer of T cells engineered to express chimeric antigen receptors (CARs) for a specific cell-surface antigen. This strategy depends upon preferential expression of the target on tumor cells. To date, the lack of AML-specific surface markers has impeded development of such CAR-based approaches. CD123, the transmembrane α chain of the interleukin-3 receptor, is expressed in the majority of AML cells but is also expressed in many normal hematopoietic cells. Here, we show that CD123 is a good target for AML-directed CAR therapy, because its expression increases over time in vivo even in initially CD123(dim) populations, and that human CD123-redirected T cells (CART123) eradicate primary AML in immunodeficient mice. CART123 also eradicated normal human myelopoiesis, a surprising finding because anti-CD123 antibody-based strategies have been reportedly well tolerated. Because AML is likely preceded by clonal evolution in "preleukemic" hematopoietic stem cells, our observations support CART123 as a viable AML therapy, suggest that CART123-based myeloablation may be used as a novel conditioning regimen for hematopoietic cell transplantation, and raise concerns for the use of CART123 without such a rescue strategy.
2-Methoxyestradiol Is an Estrogen Receptor Agonist That Supports Tumor Growth in Murine Xenograft Models of Breast Cancer (2005)

Tumor growth enhancement by 2MEO at doses generating serum levels (100-500 nmol/L) that have estrogenic activity suggests that a conservative approach to the further clinical evaluation of this agent should be adopted and that its evaluation in breast cancer is inappropriate.
Balancing push and pull for data broadcast (1997)

The increasing ability to interconnect computers through internet-working, wireless networks, high-bandwidth satellite, and cable networks has spawned a new class of information-centered applications based on data dissemination. These applications employ broadcast to deliver data to very large client populations. We have proposed the Broadcast Disks paradigm [Zdon94, Acha95b] for organizing the contents of a data broadcast program and for managing client resources in response to such a program. Our previous work on Broadcast Disks focused exclusively on the “push-based” approach, where data is sent out on the broadcast channel according to a periodic schedule, in anticipation of client requests. In this paper, we study how to augment the push-only model with a “pull-based” approach of using a backchannel to allow clients to send explicit requests for data to the server. We analyze the scalability and performance of a broadcast-based system that integrates push and pull and study the impact of this integration on both the steady state and warm-up performance of clients. Our results show that a client backchannel can provide significant performance improvement in the broadcast environment, but that unconstrained use of the backchannel can result in scalability problems due to server saturation. We propose and investigate a set of three techniques that can delay the onset of saturation and thus, enhance the performance and scalability of the system.
Background free imaging of single mRNAs in live cells using split fluorescent proteins (2014)

We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.
Carbazole/iridium dendrimer side-chain phosphorescent copolymers for efficient light emitting devices (2011)

Dendrimers have proved to be successful materials for solution-processable phosphorescent organic light emitting diodes (OLEDs). Recently we have been investigating poly(dendrimer)s with improved viscosity for ink-jet printing applications. In this work we present three phosphorescent side-chain copolymers comprised of a poly(styrene) backbone, carbazole charge transporting units, and iridium(III) complexes containing no dendrons (simple complex), a single dendron attached to two of the ligands, or doubly dendronised complexes with two dendrons added to the ligands. In addition to their charge transporting capability the copolymer structure incorporating carbazole units can also prevent aggregation of the chromophores, as evidenced by there being only one emissive environment in time-resolved luminescence. We report high external quantum efficiencies of 11.0% (37.3 cd A−1) at 100 cd m−2 and 8.3 V for devices made with the neat copolymers. The best performance is achieved by blending the doubly dendronised copolymer with 50 wt% of 4,4′-bis(N-dicarbazolyl)biphenyl (CBP) which results in 14.7% EQE (48.3 cd A−1) at 100 cd m−2 and 9.3V.
Cancer cell lines (1999)

17. Ovarian Cancer A.P. Wilson, C.M. Garner. 18. Cervical Cancer S.Y. Sharp, L.R. Kelland. 19. Endometrial Cancer P.G. Satyaswaroop. 20. Breast Cancer R.L. Sutherland, et al. 21. Paired Breast Cancer Cell Lines I.I. Wistuba, et al. 22. Ovarian Germ Cell Tumors M. Sawada, T. Miki. 23. Testicular Germ Cell Tumors M.F. Pera. 24. Choriocarcinoma V. Ganapathy, et al. 25. Thymomas and Thymic Cancers H.K. Muller-Hermelink, A. Marx. 26. Kaposi's Sarcoma C. Boshoff. 27. Brain Tumors F. Ali-Osman. 28. Head and Neck Cancers C.D. Lansford, et al. 29. Gastric Cancer T. Suzuki, M. Sekiguchi. 30. Colorectal Cancer M.G. Brattain, et al. 31. Prostate Cancer J.M. Kozlowski, J.A. Sensibar. 32. Liver Cancer M. Namba, et al. 33. Wilms' Tumor and Other Childhood Renal Neoplasms N.A. Brownlee, et al. 34. Retinoblastoma B.L. Gallie, et al.
Store-Operated Calcium Channels: How Do We Measure Them, and Why Do We Care? (2004)

A major mechanism whereby calcium entry into cells is regulated is the store-operated or capacitative calcium entry pathway. In this article, two basic issues are discussed: (i) the methods investigators use to measure store-operated entry, and (ii) the role played by the store-operated pathway in responses to hormones and neurotransmitters under physiological conditions. The two topics are considered together because they are closely interrelated; as we begin to ask questions about calcium movements at low concentrations of agonists, the technology to measure these movements becomes increasing challenging.
Design and fabrication of a 40-MHz annular array transducer (2005)

This paper investigates the feasibility of fabricating a five-ring, focused annular array transducer operating at 40 MHz. The active piezoelectric material of the transducer was a 9-microm thick polyvinylidene fluoride (PVDF) film. One side of the PVDF was metallized with gold and forms the ground plane of the transducer. The array pattern of the transducer and electrical traces to each annulus were formed on a copper-clad polyimide film. The PVDF and polyimide were bonded with a thin layer of epoxy, pressed into a spherically curved shape, then back filled with epoxy. A five-ring transducer with equal area elements and 100-microm kerfs between annuli was fabricated and tested. The transducer had a total aperture of 6 mm and a geometric focus of 12 mm. The pulse/echo response from a quartz plate located at the geometric focus, two-way insertion loss (IL), complex impedance, electrical crosstalk, and lateral beamwidth all were measured for each annulus. The complex impedance data from each element were used to perform electrical matching, and the measurements were repeated. After impedance matching; fc approximately equal to 36 MHz and -6-dB bandwidths ranged from 31 to 39%. The ILs for the matched annuli ranged from -28 to -38 dB.
Camera modeling and distortion calibration for mobile robot vision (2008)

To control the mobile robot with the surrounding information is the essential method to realize the intelligence and automatic moving. The vision information is the most important way to perceive the environment for the mobile robot. This paper presents an essential camera calibration technique for mobile robot, which is based on Pioneer II experiment platform. The technique includes transformation of coordinates system for vision system, the model and principle of image formation, camera distortion calibration. Because of non-linear distortion of camera, algorithm with optimizing operators is presented to improve calibration precision. We verify the validity and feasibility of the algorithm through experiment.
Systemic Delivery of a Novel Liver-Detargeted Oncolytic Adenovirus Causes Reduced Liver Toxicity but Maintains the Antitumor Response in a Breast Cancer Bone Metastasis Model (2011)

We are interested in developing oncolytic adenoviruses for the treatment of bone metastasis of cancer. A key limitation of systemic delivery of oncolytic adenovirus type 5 (Ad5) is that the majority of the virus is taken up by the liver, causing liver damage and systemic toxicity. Given that Ad5 hexon binding with blood coagulation factor X is a key factor in liver sequestration, and that a rare serotype, Ad48, has a diminished capacity to bind with factor X, we have generated mHAd.luc2, a novel hexon-chimeric oncolytic adenovirus. To create mHAd.luc2, seven hypervariable regions of Ad5 hexon were substituted with the corresponding regions from Ad48. Compared with Ad5-based oncolytic virus Ad.luc2, intravenous injection of mHAd.luc2 into nude mice resulted in significantly reduced liver uptake. A single high dose (1.0×10(11) viral particles/mouse) of Ad.luc2 resulted in 100% animal death by day 3; whereas none of the mice died in the mHAd.luc2 group. Liver enzyme and liver pathology studies indicated that mHAd.luc2 induced significantly less liver toxicity compared with Ad.luc2. Both mHAd.luc2 and Ad.luc2 exhibited similar binding with breast tumor cells, whereas in the presence of factor X, mHAd.luc2 binding was reduced. Both mHAd.luc2 and Ad.luc2 had nearly equal replication potential in breast cancer cells in vitro. Intravenous injection of mHAd.luc2 and Ad.luc2 into nude mice bearing bone metastases resulted in uptake of the viruses into skeletal tumors, and induced significant inhibition of established bone metastases. Thus, liver-detargeted oncolytic adenovirus can be developed for the treatment of breast cancer bone metastasis.
The human major histocompatibility complex class Ib molecule HLA‐E binds signal sequence‐derived peptides with primary anchor residues at positions 2 and 9 (1997)

Human histocompatibility leukocyte antigen E (HLA-E) and mouse major histocompatibility complex (MHC) class Ib antigen, Qa-1, share the same substitutions at two normally conserved positions 143 and 147, which are likely to affect binding of the C terminus of peptides. Qa-1 is able to bind a peptide derived from the leader sequence of H-2 D and H-2 L molecules. We developed a peptide binding assay in vitro to compare the binding specificity of HLA-E with the mouse MHC class Ib molecule Qa-1. We demonstrate that HLA-E binds, although poorly, the peptide which binds to Qa-1 and that it also binds nonamer signal sequence-derived peptides from human MHC class I molecules. Using alanine and glycine substitutions, we could define primary anchor residues at positions 2 and 9 and secondary anchor residues at position 7 and possibly 3.
Volumetric modulated arc therapy: IMRT in a single gantry arc (2007)

In this work a novel plan optimization platform is presented where treatment is delivered efficiently and accurately in a single dynamically modulated arc. Improvements in patient care achieved through image-guided positioning and plan adaptation have resulted in an increase in overall treatment times. Intensity-modulated radiation therapy (IMRT) has also increased treatment time by requiring a larger number of beam directions, increased monitor units (MU), and, in the case of tomotherapy, a slice-by-slice delivery. In order to maintain a similar level of patient throughput it will be necessary to increase the efficiency of treatment delivery. The solution proposed here is a novel aperture-based algorithm for treatment plan optimization where dose is delivered during a single gantry arc of up to 360 deg. The technique is similar to tomotherapy in that a full 360 deg of beam directions are available for optimization but is fundamentally different in that the entire dose volume is delivered in a single source rotation. The new technique is referred to as volumetric modulated arc therapy (VMAT). Multileaf collimator (MLC) leaf motion and number of MU per degree of gantry rotation is restricted during the optimization so that gantry rotation speed, leaf translation speed, and dose rate maxima do not excessively limit the delivery efficiency. During planning, investigators model continuous gantry motion by a coarse sampling of static gantry positions and fluence maps or MLC aperture shapes. The technique presented here is unique in that gantry and MLC position sampling is progressively increased throughout the optimization. Using the full gantry range will theoretically provide increased flexibility in generating highly conformal treatment plans. In practice, the additional flexibility is somewhat negated by the additional constraints placed on the amount of MLC leaf motion between gantry samples. A series of studies are performed that characterize the relationship between gantry and MLC sampling, dose modeling accuracy, and optimization time. Results show that gantry angle and MLC sample spacing as low as 1 deg and 0.5 cm, respectively, is desirable for accurate dose modeling. It is also shown that reducing the sample spacing dramatically reduces the ability of the optimization to arrive at a solution. The competing benefits of having small and large sample spacing are mutually realized using the progressive sampling technique described here. Preliminary results show that plans generated with VMAT optimization exhibit dose distributions equivalent or superior to static gantry IMRT. Timing studies have shown that the VMAT technique is well suited for on-line verification and adaptation with delivery times that are reduced to approximately 1.5-3 min for a 200 cGy fraction.
Mechanism of Dissolution Enhancement and Bioavailability of Poorly Water Soluble Celecoxib by Preparing Stable Amorphous Nanoparticles (2010)

The process by combining the antisolvent precipitation under sonication and HPH was a promising method to produce small, uniform and stable CXB nanoparticles with markedly enhanced dissolution rate and oral bioavailability due to an increased solubility that is attributed to a combination of amorphization and nanonization with increased surface area, improved wettability and reduced diffusion pathway.
Chemical Properties of Berkelium (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical Properties of BerkeliumS. G. Thompson, B. B. Cunningham, and G. T. SeaborgCite this: J. Am. Chem. Soc. 1950, 72, 6, 2798–2801Publication Date (Print):June 1, 1950Publication History Published online1 May 2002Published inissue 1 June 1950https://doi.org/10.1021/ja01162a538RIGHTS & PERMISSIONSArticle Views140Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (491 KB) Get e-Alerts Get e-Alerts
Spatial applications using 4S technology for mobile environment (2003)

The provision of spatial data is a key infrastructural requirement for the promotion of economic growth, environmental quality, social progress, etc. 4S technology integrates 4 kinds of systems that process spatial Information: GIS, GNSS, ITS, and SIIS. We use 4S technology for mobile environment and develop spatial applications. Spatial applications can be classified to 3 phases: construction, exchange, and usage. Standard specifications from OpenGIS are used to provide interoperabillty. We implement location-based services such as, fire report application for disaster management on Java phone, mobile event application, parking management system, and car navigation system.
The extracellular PHR peptide-Rap phosphatase signaling circuit of bacillus subtilis (2003)

In the field of cell-cell communication, an emerging class of extracellular signaling peptides that function intracellularly has been identified in Gram-positive bacteria. One illustrative member of this group is the Phr family of extracellular signaling peptides of Bacillus subtilis. The Phr signaling peptides are secreted by the bacterium, and then, despite the presence of intracellular peptidases, they are actively transported into the cell where they interact with intracellular receptors to regulate gene expression. The intracellular receptors are members of a family of aspartyl-phosphate phosphatases, the Rap phosphatases. These phosphatases cause the dephosphorylation of response regulator proteins, ubiquitous regulatory proteins in bacteria. Immediately downstream of the genes for the Rap phosphatases are the genes for the Phr peptides, forming rap phr signaling cassettes. There are at least seven rap phr signaling cassettes in B. subtilis, and the genome sequence of other Gram-positive, endospore-forming bacteria suggests that similar cassettes may also function in these bacteria. In B. subtilis, the rap phr cassettes regulate sporulation, genetic competence, and genes comprising the quorum response (i.e. the response to high cell density). This review will address the mechanism of extracellular Phr signaling peptide production, transport, response, and their role in quorum sensing.
relA Gene control of bacterial glycogen synthesis (1978)

Starvation of Escherichia coli K12 for an amino acid results in the stimulation of bacterial glycogen synthesis in cells containing the relA+ gene, but not in cells carrying the relA- allele. Similarly, a large difference in glycogen content is demonstrable between relA+ and relA- cells in stationary phase. It is concluded that guanosine 5',3' -bis(diphosphate) (ppGPP) or some related relA -dependent metabolite is involved in the regulation of bacterial glycogen synthesis. Detection of significant basal levels of glycogen in a relA- strain of E. coli and in unstarved relA+ C. coli indicates that relA control is not absolutely required for glycogen synthesis but serves as a signal for modulation in response to nutrient availability.