gebhart/patrepeval huggingface dataset:
Dynamical Theory of Multi-function Dry-tuned Accelerometer/gyro (1986)

In constrast to symmetric rotor of Dry-tuned GYRO (DTG), the rotor of Dry-tuned accelerometer/GYRO (D'TAG) has different transverse moments of inertia and a proper rotor pendulosity. DTG can be improved to DATG. This instrument provides measurements of angular rate about two axes and linear accelerations along two axes. The study of DTAG is a significant effect on the development of Inertia Navegation System.This paper presents the dynamical equations of DTAG. Under the condition of multi-frequency vibration, the stationary solution is computed according to the analyses of the dynamics. The feasibility of dry-tuned sensor technology for mechanizing an inertia grade four-axes multi-function senson is demonstrated.This paper also presents the condition of eliminating error torques resulting from angular vibration inputs of double spin frequency of the driving shaft. This condition is obtained by way of simple construction of single gimbal GYRO.
Expanded and Activated Natural Killer Cells for Immunotherapy of Hepatocellular Carcinoma (2016)

Viral infection of the liver is a major risk factor for hepatocellular carcinoma (HCC). Natural killer (NK) cells recognize virally infected and oncogenically transformed cells, suggesting a therapeutic role for NK-cell infusions in HCC. Using the K562-mb15-41BBL cell line as a stimulus, we obtained large numbers of activated NK cells from the peripheral blood of healthy donors. Expanded NK cells exerted remarkably high cytotoxicity against HCC cell lines, which was generally much higher than that of unstimulated or IL2-activated NK cells. In immunodeficient NOD/scid IL2RGnull mice engrafted with Hep3B, treatment with expanded NK cells markedly reduced tumor growth and improved overall survival. HCC cells exposed for 48 hours to 5 μmol/L of sorafenib, a kinase inhibitor currently used for HCC treatment, remained highly sensitive to expanded NK cells. HCC cell reductions of 39.2% to 53.8% caused by sorafenib in three cell lines further increased to 80.5% to 87.6% after 4 hours of culture with NK cells at a 1:1 effector-to-target ratio. NK-cell cytotoxicity persisted even in the presence of sorafenib. We found that NKG2D, an NK-cell-activating receptor, was an important mediator of anti-HCC activity. We therefore enhanced its signaling capacity with a chimeric NKG2D-CD3ζ-DAP10 receptor. This considerably increased the anti-HCC cytotoxicity of expanded NK cells in vitro and in immunodeficient mice. The NK expansion and activation method applied in this study has been adapted to clinical-grade conditions. Hence, these results warrant clinical testing of expanded NK-cell infusions in patients with HCC, possibly after genetic modification with NKG2D-CD3ζ-DAP10. Cancer Immunol Res; 4(7); 574-81. ©2016 AACR.
Hemojuvelin: A New Link Between Obesity and Iron Homeostasis (2011)

The adipose tissue may play an active role in systemic iron regulation and this role may be determinant in obese patients. Indeed, we reported previously that hepcidin, the iron-regulatory hormone, is expressed in adipose tissue and its messenger RNA (mRNA) expression is increased in adipose tissue of morbidly obese patients. The objectives of this study were to characterize the status of hemojuvelin (HJV), another iron-regulatory protein, within the adipose tissue of morbidly obese patients. Since cell-associated HJV acts as a coreceptor of bone morphogenetic protein (BMP) to enhance hepcidin expression in liver cells, we investigated the possible involvement of this pathway in adipose tissue in regulating hepcidin expression. HJV expression was studied in adipose tissue of morbidly obese patients. Soluble HJV blood concentrations were assessed. Hepcidin regulation through BMP pathway was investigated in cultured adipocytes. HJV was expressed both at mRNA and protein levels in adipose tissue. Moreover, its mRNA expression was highly increased in adipose tissue of obese patients and correlated with mRNA hepcidin expression levels. Interestingly, HJV expressed by adipose tissue may be effective since cultured adipocytes increased their hepcidin expression when challenged with BMP2 through Smad effectors. In addition, blood concentrations of soluble HJV were significantly increased. In conclusion, adipose tissue may influence iron homeostasis in obese patients by expressing major iron-regulatory proteins and the BMP signaling pathway could be involved in regulating hepcidin expression in this tissue.
The Fragile X Syndrome Single Strand d(CGG)n Nucleotide Repeats Readily Fold Back to Form Unimolecular Hairpin Structures (1995)

Expansion of a d(CGG)n run within the 5'-untranslated region of the X-linked human gene FMR1 blocks FMR1 transcription, delays its replication, and precipitates the fragile X syndrome. We showed previously that d(CGG)n tracts aggregate into interstrand tetrahelical complexes (Fry, M., and Loeb, L. A. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 4950-4954). Here we show that these sequences also form under physiological conditions in in vitro unimolecular hairpin structures. Folding is demonstrated by temperature-dependent mobility of d(CGG)n oligomers in a nondenaturing polyacrylamide gel, by UV-hyperchromicity of thermally denaturing oligomers, and by UV cross-linking of compact forms of d(CGG)n runs interspersed by thymidine clusters. That the compact d(CGG)n structures are unimolecular is suggested by their zero-order kinetics of formation. Diethyl pyrocarbonate modification reveals a single, 4-5 residue-long central or epicentral unpaired loop in folded d(CGG)n oligomers. The position of this loop remains unchanged by insertion of thymidine clusters into 15- or 33-mer d(CGG) tracts as indicated by KMnO4 probing of unpaired thymidines. The presence of a single loop in folded d(CGG)n oligomers and the accessibility of every guanine to dimethyl sulfate modification suggest that they are hairpin and not tetraplex structures. Modeling indicates that different d(CGG)n hairpins are stabilized by guanine-guanine Hoogsteen hydrogen bonds or by Hoogsteen and Watson-Crick bonds. If formed in vivo, d(CGG)n hairpins could entail slippage and trinucleotide expansion during replication and could obstruct FMR1 gene transcription and replication.
Oral Picropodophyllin (PPP) Is Well Tolerated In Vivo and Inhibits IGF-1R Expression and Growth of Uveal Melanoma (2008)

Oral PPP was well tolerated in vivo, caused total growth inhibition of uveal melanoma xenografts, and decreased VEGF levels in the tumors.
A Numerical Study on Static and Dynamic Characteristics of Electromagnetic Air Compressor used in Household Refrigerators (2014)

With the development of high-strength magnetic material, moving magnet linear compressors have been gradually introduced in the fields of refrigeration and cryogenic engineering, especially in Stirling. This paper presents computational investigations on the static and dynamic characteristics of a moving magnet. Equivalent magnetic circuits and finite element approaches have been used to model the moving magnet linear motor. In this paper an electromagnetic air compressor is used, where the piston which is a permanent magnet makes a suction stroke by the use of two electro magnets which is positioned at both inner and outer dead centers, and sucks air into the cylinder which is made by non-ferromagnetic material. The variation of force verses piston displacement and the Von Mises stress verses piston displacement is reported and results obtained. Finally, the method to identify optimal points of the linear compressor has been described, which is indispensable to the design and operation of moving magnet linear compressors. Keywords: Computational mechanics, refrigerator, Von-Mises, piston displacement
The Ligand Recognition Specificity of β3 Integrins (1996)

AlphaIIbbeta3 (platelet membrane glycoprotein IIb-IIIa) and alphavbeta3 are members of the beta3 subfamily of integrin adhesion receptors. A cyclic peptide, KYGC(s-s)HarGDWPC(s-s) (cHarGD), originally described by Scarborough et al. (Scarborough, R. M., Naughton, M. A., Teng, W., Rose, J. W., Phillips, D. R., Nannizzi, L., Arsten, A., Campbell, A. M., and Charo, I. F.(1993) J. Biol. Chem. 268, 1066-1073) has been employed as a high affinity ligand for alphaIIbbeta3 to examine the specificity of the beta3 integrins. cHarGD interacted with high affinity with purified alphaIIbbeta3 (Kd = 10 nM) or with platelets (Kd = 120 nM). While cHarGD was specific for alphaIIbbeta3 in the presence of Ca2+, it bound to both beta3 integrins in the presence of Mn2+. Barbourin, a snake venom disintegrin containing a reactive KGD sequence, remained alphaIIbbeta3-specific, even in the presence of Mn2+. cHarGD became cross-linked to a site in beta3 of alphaIIb beta3, which is distinct from that of RGD peptides. These results allow identification of at least four classes of beta3 ligands: Class I, represented by RGD peptides and vitronectin, react similarly with alphaIIbbeta3 and alphavbeta3; Class II, represented by cHarGD, gamma-chain peptides and fibrinogen, react with both receptors in the presence of Mn2+ but only with alphaIIbbeta3 in the presence of Ca2+; Class III, represented by barbourin, are alphaIIbbeta3-specific under all cation conditions; Class IV, represented by osteopontin, bind primarily to alphavbeta3.
Efficient vector quantization of LPC parameters at 24 bits/frame (1993)

For low bit rate speech coding applications, it is important to quantize the LPC parameters accurately using as few bits as possible. Though vector quantizers are more efficient than scalar quantizers, their use for accurate quantization of linear predictive coding (LPC) information (using 24-26 bits/frame) is impeded by their prohibitively high complexity. A split vector quantization approach is used here to overcome the complexity problem. An LPC vector consisting of 10 line spectral frequencies (LSFs) is divided into two parts, and each part is quantized separately using vector quantization. Using the localized spectral sensitivity property of the LSF parameters, a weighted LSF distance measure is proposed. With this distance measure, it is shown that the split vector quantizer can quantize LPC information in 24 bits/frame with an average spectral distortion of 1 dB and less than 2% of the frames having spectral distortion greater than 2 dB. The effect of channel errors on the performance of this quantizer is also investigated and results are reported.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template (2003)

Site-directed mutagenesis is a powerful technique by which specific changes can be generated in a target DNA molecule for either structure-function investigations or for creating and deleting endonuclease restriction sites. There have been several oligonucleotide-directed mutagenesis procedures described in the literature that allow for selection against the wild-type DNA, thereby giving enrichment in those sequences carrying the desired mutation (1–4). Collectively, these methods suffer from a number of drawbacks, including multi-enzymatic steps, the necessity for DNA precipitation, the need for convenient restriction sites, and a requirement for specialized vectors. An elegant method for achieving site-directed mutagenesis has been described by Kunkel et al. (5,6) that avoids these problems and at the same time produces a high percentage of DNA molecules with the designed mutation. This process is schematically outlined in Fig. 1. It relies on the observation that an E. coli strain lacking two key enzymes, dUTPase (dut) and uracil N-glycosylase (ung), will synthesize DNA with a small number of uracil bases substituted for thymine. Uracil containing single-stranded DNA (ssDNA) prepared from this dut − , ung − host strain can be used as a template for a mutagenic oligonucleotide to prime in vitro DNA synthesis of a complementary strand. When this heteroduplex is transformed into a wild-type (dut +, ung +) E. coli the uracil template strand is selectively inactivated, resulting in a majority of the progeny having the designed mutation.
Substituted 1,3-dihydro-2H-pyrrolo[2,3-b]pyridin-2-ones as potential antiinflammatory agents (1990)

A series of analogues based on the 1,3-dihydro-2H-pyrrolo[2,3-b]pyridin-2-one ring system have been synthesized and shown to possess oral antiinflammatory activity in both the reverse passive Arthus reaction (RPAR) pleural cavity assay in rats and in the adjuvant-induced arthritic rat model (AAR). Several members of this series additionally exhibit an inhibitory effect on the in vivo production of prostaglandin- and leukotriene-derived products or arachidonic acid metabolism although these compounds exhibit no significant inhibitory activity against the cyclooxygenase and 5-lipoxygenase enzymes in vitro. Structure-activity relationships in this series are discussed.
A Primal-Dual Interior Point Method for Optimal Power Flow Dispatching (2002)

In this paper the solution of the optimal power flow dispatching (OPFD) problem by a primal-dual interior point method is considered. Several primal-dual methods for OPF have been suggested, all of which are essentially direct extensions of primal-dual methods for linear programming. The aim of the present work is to enhance convergence through two modifications: a filter technique to guide the choice of the step length and an altered search direction in order to avoid convergence to a nonminimizing stationary point. A reduction in computational time is also gained through solving a positive definite matrix for the search direction. Numerical tests on standard IEEE systems and on a realistic network are very encouraging and show that the new algorithm converges where other algorithms fail.
Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme (2017)

Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Nevertheless, the assay conditions in many ways are nonphysiological. The term alkaline phosphatase emerged when it became necessary to distinguish "bone phosphatase" from the phosphatase in the prostate that features an acidic pH optimum. Beginning in 1948, studies of the inborn-error-of-metabolism hypophosphatasia would identify the natural substrates and establish the physiological role of alkaline phosphatase, including in biomineralization. Here, we recount the discovery in 1923 and then eventual naming of this enzyme that remains paramount in our field. © 2017 American Society for Bone and Mineral Research.
Wnt5a: its signalling, functions and implication in diseases (2011)

Wnt5a is a representative ligand that activates the β-catenin-independent pathways. Because the β-catenin-independent pathway includes multiple signalling cascades in addition to the planar cell polarity and Ca(2+) pathway, Wnt5a regulates a variety of cellular functions, such as proliferation, differentiation, migration, adhesion and polarity. Consistent with the multiple functions of Wnt5a signalling, Wnt5a knockout mice show various phenotypes, including an inability to extend the embryonic anterior-posterior and proximal-distal axes in outgrowth tissues. Thus, many important roles of Wnt5a in developmental processes have been demonstrated. Moreover, recent reports suggest that the postnatal abnormalities in the Wnt5a signalling are involved in various diseases, such as cancer, inflammatory diseases and metabolic disorders. Therefore, Wnt5a and its signalling pathways could be important targets for the diagnosis and therapy for human diseases.
Calcium/calmodulin-dependent protein kinase IV mediates acute nicotine-induced antinociception in acute thermal pain tests (2013)

Calcium-activated second messengers such as calcium/calmodulin-dependent protein kinase II have been implicated in drug-induced antinociception. The less abundant calcium-activated second messenger, calcium/calmodulin-dependent protein kinase IV (CaMKIV), mediates emotional responses to pain and tolerance to morphine analgesia but its role in nicotine-mediated antinociception is currently unknown. The goal of this study was to evaluate the role of CaMKIV in the acute effects of nicotine, primarily acute nicotine-induced antinociception. CaMKIV knockout (-/-), heterozygote (+/-), and wild-type (+/+) mice were injected with various doses of nicotine and evaluated in a battery of tests, including the tail-flick and hot-plate tests for antinociception, body temperature, and locomotor activity. Our results show a genotype-dependent reduction in tail-flick and hot-plate latency in CaMKIV (+/-) and (-/-) mice after acute nicotine treatment, whereas no difference was observed between genotypes in the body temperature and locomotor activity assessments. The results of this study support a role for CaMKIV in acute nicotine-induced spinal and supraspinal pain mechanisms, and further implicate involvement of calcium-dependent mechanisms in drug-induced antinociception.
Extracellular regulation of TGF-β activity in wound repair: growth factor latency as a sensor mechanism for injury (2004)

The transforming growth factor-beta (TGFbeta) family of growth factors are major regulators of wound repair, scar formation, and fibrosis. One of the prominent features of TGFbeta biology is the fact that this growth factor is secreted as a latent precursor, which may be directed to and stored at specific sites in the cellular microenvironment. Targeting and mobilization, and particularly extracellular activation of latent TGF-beta control the biological action of this growth factor. This review will focus on mechanisms of extracellular TGF-beta regulation relevant to and potentially operating in wound repair and scarring.
Structure and allosteric regulation of the αXβ2 integrin I domain (2003)

The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response. We now report the structure of the alpha X I domain resolved at 1.65 A by x-ray crystallography. To analyze structural requirements for ligand binding we made a mutation in the alpha X I domain C-terminal helix, which increased the affinity for iC3b approximately 200-fold to 2.4 microM compared with the wild-type domain affinity of approximately 400 microM. Gel permeation chromatography supported a conformational change between the wild-type and mutated domains. Conservation of allosteric regulation in the alpha X I domain points to the functional importance of this phenomenon.
Ultrasound-modulated fluorescence from rhodamine B aqueous solution (2010)

We report experimentally observed ultrasound-modulated fluorescence (UMF) from a submillimeter tube filled with rhodamine B aqueous solution. The tube was submerged in water and a scattering medium. Based on the measured data, we find that the UMF signals might be generated from three mechanisms: modulation of the excitation light, modulation of the emission light, and modulation of the properties of fluorophore. In addition, a linear relationship between the UMF and the drive voltage applied to the ultrasound transducer is found.
Cellular dissection of psoriasis for transcriptome analyses and the post-GWAS era (2014)

These findings underscore the role of innate immunity in psoriasis and highlight neutrophils as a cell type linked with pathogenetic mechanisms. Assignment of candidate cell types to genes emerging from GWAS studies provides a first step towards functional analysis, and we have proposed an approach for generating hypotheses to explain GWAS hits at intergenic loci.
Gender, sexual orientation, and disordered eating (1995)

Abstract While most patients with bulimia nervosa are heterosexual women, a significant number of men with bulimia nervosa are gay males, suggesting that gay men, more than heterosexual men, may be at risk for developing eating disorders. This study compared the food-related attitudes and behaviors of heterosexual men and women in contrast with those of lesbians and gay men, attempting to find whether one's sexual orientation may contribute to differential risk. Two thousand employees were randomly selected from a large health care corporation, of whom 805 returned a completed questionnaire including Stunkard and Messick's (1985) Dietary Restraint and Disinhibition Scales. A stratified random sample was drawn to include demographically comparable groups of 25 lesbians, 50 gay men, 75 heterosexual women, and 75 heterosexual men. Women were less satisfied with their appearance at their present weight and expressed more restraint and more negative feelings after bingeing than did men. Heterosexual women were most dissatisfied with their weight, although heterosexual men were more objectively overweight. Gay men and heterosexual women were similar in disordered eating patterns and disinhibition, contrasting with lesbians and heterosexual men. Key Words: Gendersexualorientation.
Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes (2011)

Many prokaryotes contain the recently discovered defense system against mobile genetic elements. This defense system contains a unique type of repetitive DNA stretches, termed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs). CRISPRs consist of identical repeated DNA sequences (repeats), interspaced by highly variable sequences referred to as spacers. The spacers originate from either phages or plasmids and comprise the prokaryotes' 'immunological memory'. CRISPR-associated (cas) genes encode conserved proteins that together with CRISPRs make-up the CRISPR/Cas system, responsible for defending the prokaryotic cell against invaders. CRISPR-mediated resistance has been proposed to involve three stages: (i) CRISPR-Adaptation, the invader DNA is encountered by the CRISPR/Cas machinery and an invader-derived short DNA fragment is incorporated in the CRISPR array. (ii) CRISPR-Expression, the CRISPR array is transcribed and the transcript is processed by Cas proteins. (iii) CRISPR-Interference, the invaders' nucleic acid is recognized by complementarity to the crRNA and neutralized. An application of the CRISPR/Cas system is the immunization of industry-relevant prokaryotes (or eukaryotes) against mobile-genetic invasion. In addition, the high variability of the CRISPR spacer content can be exploited for phylogenetic and evolutionary studies. Despite impressive progress during the last couple of years, the elucidation of several fundamental details will be a major challenge in future research.
Deflazacort for the treatment of Duchenne Dystrophy: A systematic review (2003)

Examining individual studies it appears that DFZ improves strength and functional outcomes compared to placebo, but it remains unclear if it has a benefit over prednisone on similar outcomes. Two trials found that DFZ causes less weight gain than prednisone.