gebhart/patrepeval huggingface dataset:
Inhibitors of mTOR reverse doxorubicin resistance conferred by PTEN status in prostate cancer cells. (2002)

Phosphatase and tensin homologue deleted from chromosome 10 (PTEN) is a lipid phosphatase with putative tumor suppressing abilities, which is frequently mutated in prostate cancer. Loss of PTEN leads to constitutive activation of the phosphatidylinositol 3'-kinase/serine-threonine kinase (Akt) signal transduction pathway and has been associated with resistance to chemotherapy. This study aimed to determine the effects of PTEN status and treatment with rapamycin, an inhibitor of mTOR, in the response of prostate cancer cell lines to doxorubicin. The DU-145 PTEN-positive cell line was significantly more susceptible to the antiproliferative effects of doxorubicin as compared with the PTEN-negative PC-3 cell line. Transfection of PTEN into the PC3 cells decreased the activation of Akt and the downstream mTOR-regulated 70-kDa S6 (p70(s6k)) kinase and reversed the resistance to doxorubicin in these cells, indicating that changes in PTEN status/Akt activation modulate the cellular response to doxorubicin. Treatment of PC-3 PTEN-negative cells with rapamycin inhibited 70-kDa S6 kinase and increased the proliferative response of these cells to doxorubicin, so that it was comparable with the responses of PTEN-positive DU-145 cells and the PC-3-transfected cells. Furthermore, treatment of mice bearing the PTEN-negative PC-3 prostate cancer xenografts with CCI-779, an ester of rapamycin in clinical development combined with doxorubicin, inhibited the growth of the doxorubicin-resistant PC-3 tumors confirming the observations in vitro. Thus, rapamycin and CCI-779, by interacting with downstream intermediates in the phosphatidylinositol 3'-kinase/Akt signaling pathway, reverse the resistance to doxorubicin conferred by PTEN mutation/Akt activation. These results provide the rationale to explore in clinical trials whether these agents increase the response to chemotherapy of patients with PTEN-negative/Akt active cancers.
Inhibition of HIV-1 Replication in Lymphocytes by Mutants of the Rev Cofactor eIF-5A (1996)

Eukaryotic initiation factor 5A(eIF-5A) is a cellular cofactor require d for the function of the human immunodeficiency virus type-1 (HIV-1) Rev trans-activator protein. The majority of a set of eIF-5A mutants did not support growth of yeast cells having an inactivated genomic copy of eIF-5A, indicating that the introduced mutation eliminated eIF-5A activity. Two nonfunctional mutants, eIF-5AM13 and eIF-5AM14, retained their binding capacity for the HIV-1 Rev response element:Rev complex. Both mutants were constitutively expressed in human T cells. When these T cells were infected with replication-competent HIV-1, virus replication was inhibited. The eIF-5AM13 and eIF5AM14 proteins blocked Rev trans-activation and Rev-mediated nuclear export.
Noninvasive Prenatal Molecular Karyotyping from Maternal Plasma (2013)

Fetal DNA is present in the plasma of pregnant women. Massively parallel sequencing of maternal plasma DNA has been used to detect fetal trisomies 21, 18, 13 and selected sex chromosomal aneuploidies noninvasively. Case reports describing the detection of fetal microdeletions from maternal plasma using massively parallel sequencing have been reported. However, these previous reports were either polymorphism-dependent or used statistical analyses which were confined to one or a small number of selected parts of the genome. In this report, we reported a procedure for performing noninvasive prenatal karyotyping at 3 Mb resolution across the whole genome through the massively parallel sequencing of maternal plasma DNA. This method has been used to analyze the plasma obtained from 6 cases. In three cases, fetal microdeletions have been detected successfully from maternal plasma. In two cases, fetal microduplications have been detected successfully from maternal plasma. In the remaining case, the plasma DNA sequencing result was consistent with the pregnant mother being a carrier of a microduplication. Simulation analyses were performed for determining the number of plasma DNA molecules that would need to be sequenced and aligned for enhancing the diagnostic resolution of noninvasive prenatal karyotyping to 2 Mb and 1 Mb. In conclusion, noninvasive prenatal molecular karyotyping from maternal plasma by massively parallel sequencing is feasible and would enhance the diagnostic spectrum of noninvasive prenatal testing.
Characteristics of a broad-band wide-scan fragmented aperture phased array antenna (2006)

In this paper a phased array antenna is described that is designed with the help of a genetic algorithm for scan angles out to 60° from broadside. The focus is on simplifying the manufacturing of the diagonally adjoining pixels and on numerical results for thin antennas. The effects of the corner to corner contacts are studied and results for two synthesized elements ar Test and measurement requirements on wireless devices
Bipyridyl oxadiazoles as efficient and durable electron-transporting and hole-blocking molecular materials (2005)

We have demonstrated new and efficient electron transporting (ET) and hole blocking (HB) materials for organic light-emitting devices (OLEDs). The bipyridyl-substituted oxadiazole derivatives can form a stable glassy state with glass transition temperatures greater than 100 °C. The bipyridyl-substituted oxadiazole derivatives have excellent ET, HB and electron accepting ability and also exhibit electrical operation durability. Bipyridyl-substituted oxadiazoles are a promising candidate for ET and HB materials for OLEDs.
UNB Neutral Atmosphere Models: Development and Performance (2006)

Several hybrid neutral atmosphere delay models have been developed by UNB researchers over the past decade or so. The most widely applicable current version is UNB3, which uses the Saastamoinen zenith delays, Niell mapping functions, and a look-up table with annual mean and amplitude for temperature, pressure, and water vapour pressure varying with respect to latitude and height. These parameters are computed for a particular latitude and day of year using a cosine function for the annual variation and a linear interpolation for latitude. The UNB3 model has been extensively used in several regions of the world, being capable of predicting total zenith delays with average uncertainties of 5 cm under normal atmospheric conditions. A modified version of UNB3 has been used in GPS receivers utilizing the Wide Area Augmentation System and other space-based augmentation systems. Other versions of the UNB neutral atmosphere model have been developed aiming at getting better predictions for the non-hydrostatic delay component. One of the new versions is UNB3m, whose performance has been investigated using radiosonde data and compared to that of UNB3. Based on ray-tracing analyses of 703,711 profiles from 223 stations in North America and surrounding territory from 1990 to 1996, the prediction errors of UNB3m have a mean value -0.5 cm and standard deviation of 4.9 cm. Although the standard deviation of the prediction error of UNB3m is similar to that of UNB3, the absolute mean error has been reduced by almost 75%.
Elevated Bile Acid Serum Concentrations in Uremics (1996)

Letters| December 18 2008 Elevated Bile Acid Serum Concentrations in Uremics Subject Area: Nephrology P.L. Balestri; P.L. Balestri aClinica Medica II, Search for other works by this author on: This Site PubMed Google Scholar A. Cupisti A. Cupisti bClinica Medica I, Università di Pisa, Italia Search for other works by this author on: This Site PubMed Google Scholar Nephron (1996) 72 (2): 325. https://doi.org/10.1159/000188867 Article history Published Online: December 18 2008 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation P.L. Balestri, A. Cupisti; Elevated Bile Acid Serum Concentrations in Uremics. Nephron 1 February 1996; 72 (2): 325. https://doi.org/10.1159/000188867 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsNephron Search Advanced Search Article PDF first page preview Close Modal This content is only available via PDF. 1996Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. You do not currently have access to this content.
CD8 T CELL-MEDIATED REJECTION OF INTESTINAL ALLOGRAFTS IS RESISTANT TO INHIBITION OF THE CD40/CD154 COSTIMULATORY PATHWAY (2001)

These data suggest that CD4+ T cells mediating intestinal allograft rejection may be more dependent upon the CD40/CD154 pathway than CD8+ T cells. This finding highlights the importance of identifying agents that suppress CD8+ T cell-mediated rejection.
Transversal phase resolved polarization sensitive optical coherence tomography (2004)

We present a novel optical coherence tomography (OCT) method to measure backscattered intensity and birefringence properties (retardation and fast axis orientation) and apply it to imaging of human ocular tissue. The method is based on a Mach Zehnder interferometer, on transversal scanning, and on a polarization sensitive two-channel detection. A highly stable carrier frequency is generated by acousto-optic modulators (AOMs). This allows a phase sensitive demodulation by the lock-in technique. Since the recording of individual interference fringes is avoided by this method the amount of data to be recorded and processed is considerably reduced. We demonstrate this method on human cornea and anterior chamber angle and present, to the best of our knowledge, the first OCT images of retardation and fast axis orientation of the anterior chamber angle region in vivo.
Insulin and Insulin-Like Growth Factor-I Inhibit Thyrotropin-Increased Iodide Transport in Serum-Depleted FRTL-5 Rat Thyroid Cells: Modulation of Adenosine 3′,5′-Monophosphate Signal Action (1991)

Insulin enhances the ability of TSH to induce iodide uptake in FRTL-5 rat thyroid cells maintained in 5% serum; however, in cells maintained in 0.2% serum, insulin inhibits the ability of TSH to induce iodide uptake. Since the inhibitory action of insulin is duplicated by 100-fold lower concentrations of insulin-like growth factor-I (IGF-I), inhibition appears to be mediated by the IGF-I receptor. Insulin and IGF-I inhibit the action of a cAMP analog to induce iodide uptake in a manner identical to TSH, but do not inhibit basal or TSH-increased cAMP levels; inhibition, thus, results from regulation of cAMP signal action rather than cAMP signal generation. Inhibition is associated with a more than 2-fold decrease in the maximum velocity of iodide influx, a less than 15% change in the rate of iodide efflux, and no change in the Km for iodide influx, i.e. inhibition effectively results from a decrease in the number of iodide porters. The inhibitory action of insulin/IGF-I is not additive with hydrocortisone, which, under the same conditions, also inhibits TSH- or cAMP-induced iodide porter activity. Actinomycin-D, given 24 h after TSH, superinduces TSH-induced iodide porter activity and abolishes the inhibition by insulin, IGF-I, and/or hydrocortisone; a similar paradoxical effect of actinomycin-D under these conditions has been explained by its ability to inhibit the action of a cAMP-induced factor that increases mRNA degradation. The inhibitory actions of insulin, IGF-I, and hydrocortisone on cAMP-induced iodide porter activity contrast with their simultaneous and synergistic stimulation of the transcriptional action of cAMP on DNA and thyroglobulin synthesis under these conditions.
Oncogene Suppression by Small Interfering RNAs (2004)

Almost all human cancers have accumulated multiple genetic lesions including oncogenes. It is often unknown whether an oncogene is continuously required for tumorigenesis. Furthermore, it is very difficult to target an essential oncogene with drugs without affecting the corresponding nonmutated protooncogene or related factors. The recent discovery of RNA interference and the application of small interfering RNAs in mammalian cell culture provide now tools to examine the role of oncogenes in tumor development. Furthermore, oncogene-specific siRNAs may become promising candidates for more cancer-specific therapeutic approaches. This review discusses the potential and the limitations of oncogene-targeting siRNAs and describes examples for the application of siRNAs in the functional analysis of oncogenes.
Low urinary indoxyl sulfate levels early after transplantation reflect a disrupted microbiome and are associated with poor outcome (2015)

Indole, which is produced from l-tryptophan by commensal bacteria expressing tryptophanase, not only is an important intercellular signal in microbial communities, but also modulates mucosal barrier function and expression of pro- and anti-inflammatory genes by intestinal epithelial cells. Here, we hypothesized that decreased urinary excretion of 3-indoxyl sulfate (3-IS), the major conjugate of indole found in humans, may be a marker of gut microbiota disruption and increased risk of developing gastrointestinal (GI) graft-versus-host-disease. Using liquid chromatography/tandem mass spectrometry, 3-IS was determined in urine specimens collected weekly within the first 28 days after allogeneic stem cell transplantation (ASCT) in 131 patients. Low 3-IS levels within the first 10 days after ASCT were associated with significantly higher transplant-related mortality (P = .017) and worse overall survival (P = .05) 1 year after ASCT. Least absolute shrinkage and selection operator regression models trained on log-normalized counts of 763 operational taxonomic units derived from next-generation sequencing of the hypervariable V3 region of the 16S ribosomal RNA gene showed members of the families of Lachnospiraceae and Ruminococcaceae of the class of Clostridia to be associated with high urinary 3-IS levels, whereas members of the class of Bacilli were associated with low 3-IS levels. Risk factors of early suppression of 3-IS levels were the type of GI decontamination (P = .01), early onset of antibiotic treatment (P = .001), and recipient NOD2/CARD15 genotype (P = .04). In conclusion, our findings underscore the relevance of microbiota-derived indole and metabolites thereof in mucosal integrity and protection from inflammation.
Generation of High-Yielding Influenza A Viruses in African Green Monkey Kidney (Vero) Cells by Reverse Genetics (2004)

Influenza A viruses are the cause of annual epidemics of human disease with occasional outbreaks of pandemic proportions. The zoonotic nature of the disease and the vast viral reservoirs in the aquatic birds of the world mean that influenza will not easily be eradicated and that vaccines will continue to be needed. Recent technological advances in reverse genetics methods and limitations of the conventional production of vaccines by using eggs have led to a push to develop cell-based strategies to produce influenza vaccine. Although cell-based systems are being developed, barriers remain that need to be overcome if the potential of these systems is to be fully realized. These barriers include, but are not limited to, potentially poor reproducibility of viral rescue with reverse genetics systems and poor growth kinetics and yields. In this study we present a modified A/Puerto Rico/8/34 (PR8) influenza virus master strain that has improved viral rescue and growth properties in the African green monkey kidney cell line, Vero. The improved properties were mediated by the substitution of the PR8 NS gene for that of a Vero-adapted reassortant virus. The Vero growth kinetics of viruses with H1N1, H3N2, H6N1, and H9N2 hemagglutinin and neuraminidase combinations rescued on the new master strain were significantly enhanced in comparison to those of viruses with the same combinations rescued on the standard PR8 master strain. These improvements pave the way for the reproducible generation of high-yielding human and animal influenza vaccines by reverse genetics methods. Such a means of production has particular relevance to epidemic and pandemic use.
Selective killing of squamous carcinoma cells by an immunotoxin that recognizes the egf receptor (1989)

Abstract We have conjugated a murine monoclonal antibody (B4G7) against the human epidermal growth factor (EGF) receptor to gelonin, a 60S ribosome inactivating protein, via N‐succinimidyl‐3‐(2‐pyridyldithio)propionate (SPDP) and 2‐iminothiolane. The B4G7‐gelonin conjugate bound to the cell surface in proportion to the number of EGF receptors and competed with B4G7 antibody for binding to EGF receptors. The conjugate killed EGF receptor‐hyperproducing squamous carcinoma cells (A431, NA, Ca9‐22, TE5), and to some extent, human fibroblasts (HFO). It did not kill EGF receptor‐deficient small‐cell lung cancer cells (H69) and mouse fibroblasts (Swiss/3T3). Free B4G7, gelonin or a mixture of B4G7 and gelonin did not kill A431 cells. The number of EGF receptors was correlated to cytotoxicity at 10 −8 M of the conjugate, and the data were fitted to the regression equation: y = –35.83 log x + 233.4 (correlation coefficient = –0.9995). These results suggest that the B4G7‐gelonin conjugate may be a useful weapon for targeting therapy to squamous‐cell carcinomas.
Flash photolytic investigation of photoinduced carbon monoxide dissociation from dinuclear manganese carbonyl compounds (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFlash photolytic investigation of photoinduced carbon monoxide dissociation from dinuclear manganese carbonyl compoundsRichard S. Herrick and Theodore L. BrownCite this: Inorg. Chem. 1984, 23, 26, 4550–4553Publication Date (Print):December 1, 1984Publication History Published online1 May 2002Published inissue 1 December 1984https://pubs.acs.org/doi/10.1021/ic00194a028https://doi.org/10.1021/ic00194a028research-articleACS PublicationsRequest reuse permissionsArticle Views261Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Information Services and the Music Therapy Curriculum: New Avenues, New Possibilities (1984)

This paper discusses the role of information services content in the music therapy curriculum. The first part of the paper focuses on the scope of the music therapy knowledge bare as it pertains to information servicer. The second part discusses two possible avenues of approach in respect to increasing information services content in the music therapy curriculum: (a) building information services content into the structure and organization of music therapy courses (employing several examples, utilizing citation indexing), and (b) utilizing the services of the librarian as an integral part of an information services team. Care examples can be utilized as effective learning devices about information services, such as the use of citation indexing in assessing the work of noted contributors to the field or the impact of movements in the field. The librarian and the instructor could work together as a team in respect to drawing library and information services in music therapy closer to the music therapy student. The objective here is to build an “information consciousness” whereby the student or the practitioner is aware of the many information services available in music therapy, and is proficient in using those services in the interests of improved knowledge and practice skills.
Time-Reversal Acoustics in Biomedical Engineering (2003)

Time reversal is a very powerful method for focusing through complex and heterogeneous media and shows very promising results in biomedical applications. In this paper, we review some of the main applications investigated during the past decade. An iterative implementation of the time-reversal process allows tracking gallstones in real time during lithotripsy treatments. In this application domain, a smart exploitation of the reverberations in solid waveguides permits the focusing of high-amplitude ultrasonic shock waves with a small number of transducers. Finally, because time reversal is able to correct the strong distortions induced by the skull bone on ultrasonic propagation, this adaptive focusing technique is very promising for ultrasonic hyperthermia brain therapy.
Performance Estimation of Multis treamed, Superscalar Processors (1994)

Multistreamed processors can significantly improve processor throughput by allowing interleaved execution of instructions from multiple instruction streams. In this paper, we present an analytical modeling technique to evaluate the effect of dynamically interleaving additional instruction streams within superscalar architectures. Using this technique, estimates of the instructions executed per cycle (IPC) for a processor architecture are quickly calculated given simple descriptions of the workload and hardware characteristics. To validate this technique, estimates of the SPEC89 benchmark suite obtainedfrom the model are compared to results from a hardware simulator. Our results show the technique produces accurate estimates with an average deviation of -4% from the simulation results. Finally, we demonstrate that as the number of functional units increases, multistreaming is an effective technique to expbir these additional resources.