gebhart/patrepeval huggingface dataset:
Mechanism of L-lactic Acid Transport in L6 Skeletal Muscle Cells (2004)

L-lactic acid transport plays an important role in the regulation of L-lactic acid circulation into and out of muscle. To clarify the transport mechanism of L-lactic acid in skeletal muscle, L-lactic acid uptake was investigated using a L6 cell line. mRNAs of monocarboxylate transporter (MCT) 1, 2 and 4 were found to be expressed in L6 cells. The [(14)C] L-lactic acid uptake by L6 cells increased up to pH of 6.0. The [(14)C] L-lactic acid uptake at pH 6.0 was concentration-dependent with a K(m) of 3.7 mM. This process was reduced by alpha-cyano-4-hydroxycinnamate, a typical MCT1, 2 and 4 inhibitor. These results suggest that an MCT participates in the uptake of L-lactic acid by L6 cells. [(14)C] L-lactic acid uptake was markedly inhibited by monocarboxylic acids and monocarboxylate drugs but not by dicarboxylic acids and amino acids. Moreover, benzoic acid, a substrate for MCT1, competitively inhibited this process with K(i) of 1.7 mM. [(14)C] L-lactic acid efflux in L6 cells was inhibited by alpha-cyano-4-hydroxycinnamate but not by benzoic acid. These results suggest that [(14)C] L-lactic acid efflux in L6 cells is mediated by MCT other than MCT1.
Characterization and Molecular Cloning of a Novel MUC1 Protein, Devoid of Tandem Repeats, Expressed in Human Breast Cancer Tissue (1994)

The human breast cancer marker protein, MUC1, is a polymorphic transmembrane molecule containing a large extracellular domain that is primarily composed of a variable number of highly conserved 20-amino-acid tandem repeats. We report here the detection of a novel invariantly sized 1.2-kb MUC1 mRNA, in addition to the large polymorphic mRNAs, by probing Northern blots with MUC1-cDNA-unique-sequence probes. The nucleotide sequence of this novel MUC1 mRNA demonstrates that it is identical to the MUC1 cDNA sequences downstream and upstream to the tandem-repeat array of the transmembrane form of MUC1. However, it contains neither the central tandem repeat array itself nor its directly flanking sequences that are deleted by a differential splicing event utilizing splice acceptor and donor sequences 5' and 3' to the tandem-repeat array. The splice event retains, downstream to the splice acceptor site, an open reading frame identical to that of the repeat-array-containing MUC1 thereby generating the novel MUC1/Y protein. Cells transiently transfected with the novel MUC1/Y cDNA express the MUC1/Y protein that is modified by glycosylation. The MUC1/Y protein is also readily detected in human breast cancer cells grown in vitro. Furthermore, primary breast cancer tissue samples demonstrate significant levels of the MUC1/Y protein whereas expression in tissue adjacent to the tumor is undetectable. Molecular characterization presented here, of the novel MUC1/Y molecule lacking the repeat array, suggests that it is likely to play a role distinct to that of the polymorphic repeat-array-positive MUC1 protein and that it may act as a new marker protein for human breast cancer.
T cell sensitivity to HLA class I‐mediated apoptosis is dependent on interleukin‐2 and interleukin‐4 (1997)

Antibody interaction with a specific epitope of the HLA class I alpha1 domain triggers apoptosis of activated but not resting T and B cells by a pathway which involves neither Fas ligand nor tumor necrosis factor-alpha. We have investigated at which stage of activation and proliferation T cells become sensitive to HLA class I-mediated apoptosis, using two monoclonal antibodies (mAb) which recognize the same monomorphic epitope of the HLA class I alpha1 domain (mAb9O, mouse IgG1, and YTH862, rat IgG2b) and can induce apoptosis of phytohemagglutinin (PHA)-activated peripheral blood lymphocytes. Sensitivity to apoptosis develops after the expression of G1 markers (CD69 expression) but it is accelerated by addition of recombinant interleukin-2 (rIL-2). Blocking the IL-2 pathway by cyclosporin A, FK506, rapamycin, anti-IL-2 or CD25 antibodies, prevented the development of sensitivity to apoptosis. Addition of IL-2 and, to a lesser extent, IL-4, reversed the inhibitory effect of cyclosporin A. Conversely, rIL-7 and recombinant interferon-gamma restored proliferation of peripheral blood lymphocytes stimulated by PHA in the presence of cyclosporin A but did not restore sensitivity to class I-mediated apoptosis. Finally cells stimulated in the presence of the DNA polymerase inhibitor aphidicolin did not enter into S phase of the cell cycle but secreted IL-2 and underwent apoptosis when exposed to mAb90 or YTH862. Together, the data indicate that sensitivity of peripheral T cells to HLA class I-mediated apoptosis depends on both activation signals and IL-2 or IL-4, but does not require cell proliferation. These data suggest that YTH862 and mAb90 might be used for achieving clonal deletion of antigen-activated peripheral T cells in vivo, provided that the IL-2 pathway is not blocked by other immunosuppressive agents.
Growth and Properties of Hg1−xCdxTe Epitaxial Layers (1969)

The epitaxial growth of Hg1−xCdxTe has been investigated by means of a technique in which the source and substrate are close spaced, and both under isothermal conditions and with a temperature gradient between the source and substrate. The effect of excess mercury pressure in the ampoule on the growth rate and the compositional profile within the isothermally grown epitaxial layers has been investigated in detail. A simple isothermal process is described for making epitaxial layers having a controlled surface alloy composition. The presence of a temperature gradient between the source and the substrate significantly enhances the deposition rate and results in a linear relation between the layer thickness and deposition time. The electrical and optical properties of the isothermally grown epitaxial layers are discussed along with the results of photoconductivity measurements on layers having surface composition of x=0.20 and x=0.25.
ON THE G(F)-SEQUENCE OF A CW-TRIPLE (1999)

We find some conditions under which G(f)-sequence of a CW-pair (X, A) is exact. And we also introduce a G(f)-sequence for a CW-triple (X, A, B) and examine when the sequence is exact.
Use of Antibiotics for Selective Isolation and Enumeration of Actinomycetes in Soil (1965)

SUMMARY: Several antibiotics were tested against a range of actinomycetes, bacteria and fungi representing types found in soil. From these tests four antibiotics, nystatin (50 μg./ml.), actidione (50 μg./ml.), polymyxin B sulphate (5·0 μg./ml.) and sodium penicillin (1·0 μg./ml.), were selected for incorporation into a starch + casein medium to achieve selective growth of actinomycetes on soil dilution plates. This mixture of antibiotics was tested with a number of soils and its efficiency compared with several other methods for selective development of actinomycete colonies. The most suitable mixture for the enumeration of soil actinomycete colonies was starch + casein medium with the two antifungal antibiotics (nystatin, actidione); for isolation of actinomycete colonies the same medium with all four antibiotics was the most satisfactory.
Aminobenzoic acid diuretics. 2. 4-Substituted-3-amino-5-sulfamoylbenzoic acid derivatives (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAminobenzoic acid diuretics. 2. 4-Substituted-3-amino-5-sulfamoylbenzoic acid derivativesPeter W. FeitCite this: J. Med. Chem. 1971, 14, 5, 432–439Publication Date (Print):May 1, 1971Publication History Published online1 May 2002Published inissue 1 May 1971https://pubs.acs.org/doi/10.1021/jm00287a014https://doi.org/10.1021/jm00287a014research-articleACS PublicationsRequest reuse permissionsArticle Views426Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development and Application of an Ultrasensitive Hybridization-Based ELISA Method for the Determination of Peptide-Conjugated Phosphorodiamidate Morpholino Oligonucleotides (2015)

Antisense oligonucleotide (AON)-induced exon skipping is one of the most promising strategies for treating Duchenne muscular dystrophy (DMD) and other rare monogenic conditions. Phosphorodiamidate morpholino oligonucleotides (PMOs) and 2'-O-methyl phosphorothioate (2'OMe) are two of the most advanced AONs in development. The next generation of peptide-conjugated PMO (P-PMO) is also showing great promise, but to advance these therapies it is essential to determine the pharmacokinetic and biodistribution (PK/BD) profile using a suitable method to detect AON levels in blood and tissue samples. An enzyme-linked immunosorbent assay (ELISA)-based method, which shows greater sensitivity than the liquid chromatography-mass spectrometry method, is the method of choice for 2'OMe detection in preclinical and clinical studies. However, no such assay has been developed for PMO/P-PMO detection, and we have, therefore, developed an ultrasensitive hybridization-based ELISA for this purpose. The assay has a linear detection range of 5-250 pM (R(2)>0.99) in mouse serum and tissue lysates. The sensitivity was sufficient for determining the 24-h PK/BD profile of PMO and P-PMO injected at standard doses (12.5 mg/kg) in mdx mice, the dystrophin-deficient mouse model for DMD. The assay demonstrated an accuracy approaching 100% with precision values under 12%. This provides a powerful cost-effective assay for the purpose of accelerating the development of these emerging therapeutic agents.
Rha1, an Arabidopsis Rab5 Homolog, Plays a Critical Role in the Vacuolar Trafficking of Soluble Cargo Proteins (2003)

Rab proteins are members of the Ras superfamily of small GTP binding proteins and play important roles in various intracellular trafficking steps. We investigated the role of Rha1, an Arabidopsis Rab5 homolog, in intracellular trafficking in Arabidopsis protoplasts. In the presence of a dominant-negative mutant of Rha1, soluble vacuolar cargo proteins such as sporamin:green fluorescent protein (Spo:GFP) and Arabidopsis aleurain like protein:GFP are not delivered to the central vacuole; instead, they accumulate as a diffuse or punctate staining pattern within the cell. Spo:GFP at the punctate stains observed in the presence of hemagglutinin:Rha1[S24N] is colocalized with endogenous vacuolar sorting receptor (VSR(At-1)), which is known to localize primarily to the prevacuolar compartment, whereas Spo:GFP in the diffuse pattern is associated with tonoplasts. Furthermore, expression of Rha1[S24N] causes the secretion of a portion of the vacuolar proteins into medium. However, the inhibitory effect of Rha1[S24N] on vacuolar trafficking is relieved partially by coexpressed wild-type Rha1. Based on these results, we propose that Rha1 plays a critical role in the trafficking of soluble cargoes from the prevacuolar compartment to the central vacuole.
THE GENERAL ADAPTATION SYNDROME AND THE DISEASES OF ADAPTATION1 (1946)

SINCE the first description of the “alarm reaction,” a decade ago, many publications have dealt with this phenomenon and with the “general adaptation syndrome,” of which it forms a part. It is becoming increasingly more obvious that certain physiologic mechanisms, in which the endocrin system plays a prominent part, help to raise resistance to damage as such, irrespective of the specific nature of the damaging agents. Interest in the general adaptation syndrome has recently received a further impetus as a result of investigations suggesting that some of the most important diseases of human pathology (such as hypertension, nephrosclerosis and the rheumatic diseases) may represent by-products of the endocrine reactions, which are at play in the general adaptation syndrome. It was considered a timely enterprise, therefore, to survey this field now and to supply a guide to the rather scattered and polyglot, pertinent literature. In order to familiarize himself with different points of view, the reader is also referred to several earlier reviews on the general adaptation syndrome (10, 11, 235, 295, 358, 569, 571, 577, 629, 634, 654).
Epithelial-Mesenchymal Transition Events during Human Embryonic Stem Cell Differentiation (2007)

Epithelial-mesenchymal transition (EMT) occurs during embryonic development and may also be associated with the metastatic spread of epithelial tumors. During EMT, E-cadherin is down-regulated and this correlates with increased motility and invasion of cells. We show that differentiation of human embryonic stem (ES) cells in monolayer culture is associated with an E- to N-cadherin switch, increased vimentin expression, up-regulation of E-cadherin repressor molecules (Snail and Slug proteins), and increased gelatinase (matrix metalloproteinases; MMP-2 and MMP-9) activity and cellular motility, all characteristic EMT events. The 5T4 oncofetal antigen, previously shown to be associated with early human ES cell differentiation, is also part of this process. Abrogation of E-cadherin-mediated cell-cell contact in undifferentiated ES cells using neutralizing antibody (nAb) SHE78.7 resulted in increased cellular motility, altered actin cytoskeleton arrangement and a mesenchymal phenotype together with presentation of the 5T4 antigen at the cell surface. nAb-treated ES cells remained in an undifferentiated state, as assessed by OCT-4 protein expression, and did not express EMT-associated transcripts. Removal of nAb from ES cells resulted in the restoration of cell-cell contact, absence of cell surface 5T4, decreased mesenchymal cellular morphology and motility, and enabled the differentiation of the cells to the three germ layers upon their removal from the fibroblast feeder layer. We conclude that E-cadherin functions in human ES cells to stabilize the cortical actin cyoskeletal arrangement and this prevents cell surface localization of the 5T4 antigen. Furthermore, human ES cells represent a useful model system with which to study EMT events relevant to embryonic development and tumor cell metastasis.
Enabling fuel cells for standby power - chemical hydride fueling technology (2005)

Early market entry versions of PEM fuel cells and their attendant fueling systems are now being deployed with early adopters to evaluate system performance, suitability, and reliability for backup power applications for telecom and related critical power systems. One important barrier to the widespread use of PEM fuel cells will be deployment of hydrogen fuel systems to supply the fuel cell. While there are several options in this area, as yet no commercial system can claim to provide a completely satisfactory and cost effective solution. Fueling approaches fall into two categories: storage of preproduced hydrogen or on-demand production of hydrogen. In the former category, storage in steel cylinders provided by merchant suppliers is the benchmark, incumbent technology. Alternatives such as storage of cryogenic hydrogen or storage in reversible metal hydride systems are less desirable. Options where hydrogen is produced on site and then stored are also less effective. "On-demand" production of hydrogen has the key advantage of reducing hydrogen inventories which otherwise cause siting issues because of fire codes and workplace standards. It also reduces dependence on specialized distribution channels for compressed gasses and eliminates the large, heavy packaging (cylinders) required for compressed gas distribution. While there are relatively few viable options for on-demand fuel processing, this paper makes the case that the leading option, relying on rapid, catalyzed processing of mixtures of water and reactive chemical hydrides will provide a value-added, cost-effective solution.
Re-Introduction of communication-avoiding FMM-accelerated FFTs with GPU acceleration (2013)

As distributed memory systems grow larger, communication demands have increased. Unfortunately, while the costs of arithmetic operations continue to decrease rapidly, communication costs have not. As a result, there has been a growing interest in communication-avoiding algorithms for some of the classic problems in numerical computing, including communication-avoiding Fast Fourier Transforms (FFTs). A previously-developed low-communication FFT, however, has remained largely out of the picture, partially due to its reliance on the Fast Multipole Method (FMM), an algorithm that typically aids in accelerating dense computations. We have begun an algorithmic investigation and re-implementation design for the FMM-accelerated FFT, which exploits the ability to tune precision of the result (due to the mathematical nature of the FMM) to reduce power-burning communication and computation, the potential benefit of which is to reduce the energy required for the fundamental transform of digital signal processing. We reintroduce this algorithm as well as discuss new innovations for separating the distinct portions of the FMM into a CPU-dedicated process, relying on inter-processor communication for approximate interactions, and a GPU-dedicated process for dense interactions with no communication <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> .
Light-weight localization for vehicles using road markings (2013)

Traditional vision-based localization methods such as visual SLAM suffer from practical problems in outdoor environments such as unstable feature detection and inability to perform location recognition under lighting, perspective, weather and appearance change. Additionally map construction on a large scale in these systems presents its own challenges. In this work, we present a novel method for precisely localizing vehicles on the road using signs marked on the road (road markings), which have the advantage of being distinct and easy to detect, their detection being robust under changes in lighting and weather. Our method uses corners detected on road markings to perform localization in global coordinates. The method consists of two phases - a mapping phase when a high-quality GPS device is used to automatically survey road marks and add them to a light-weight “map” or database, and a localization phase where road mark detection and look-up in the map, combined with visual odometry, produces precise localization. We present experiments using a real-time implementation operating in a car that demonstrates the improved localization robustness and accuracy of our system even when using road marks alone. However, in this case the trajectory between road marks has to be filled-in by visual odometry, which contributes drift. Hence, we also present a mechanism for combining road-mark-based maps with sparse feature-based maps that results in greater accuracy still. We see our use of road marks as a significant step in the general trend of using higher-level features for improved localization performance irrespective of environment conditions.
Age‐Related Macular Degeneration (2013)

Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly, and the prevalence of the disease increases exponentially with every decade after age 50 years. It is a multifactorial disease involving a complex interplay of genetic, environmental, metabolic, and functional factors. Besides smoking, hypertension, obesity, and certain dietary habits, a growing body of evidence indicates that inflammation and the immune system may play a key role in the development of the disease. AMD may progress from the early form to the intermediate form and then to the advanced form, where two subtypes exist: the nonneovascular (dry) type and the neovascular (wet) type. The results from the Age-Related Eye Disease Study have shown that for the nonneovascular type of AMD, supplementation with high-dose antioxidants (vitamin C, vitamin E, and β-carotene) and zinc is recommended for those with the intermediate form of AMD in one or both eyes or with advanced AMD or vision loss due to AMD in one eye. As for the neovascular type of the advanced AMD, the current standard of therapy is intravitreal injections of vascular endothelial growth factor inhibitors. In addition, lifestyle and dietary modifications including improved physical activity, reduced daily sodium intake, and reduced intake of solid fats, added sugars, cholesterol, and refined grain foods are recommended. To date, no study has demonstrated that AMD can be cured or effectively prevented. Clearly, more research is needed to fully understand the pathophysiology as well as to develop prevention and treatment strategies for this devastating disease.
Mitochondrial Membrane Permeabilization in Cell Death (2007)

Irrespective of the morphological features of end-stage cell death (that may be apoptotic, necrotic, autophagic, or mitotic), mitochondrial membrane permeabilization (MMP) is frequently the decisive event that delimits the frontier between survival and death. Thus mitochondrial membranes constitute the battleground on which opposing signals combat to seal the cell's fate. Local players that determine the propensity to MMP include the pro- and antiapoptotic members of the Bcl-2 family, proteins from the mitochondrialpermeability transition pore complex, as well as a plethora of interacting partners including mitochondrial lipids. Intermediate metabolites, redox processes, sphingolipids, ion gradients, transcription factors, as well as kinases and phosphatases link lethal and vital signals emanating from distinct subcellular compartments to mitochondria. Thus mitochondria integrate a variety of proapoptotic signals. Once MMP has been induced, it causes the release of catabolic hydrolases and activators of such enzymes (including those of caspases) from mitochondria. These catabolic enzymes as well as the cessation of the bioenergetic and redox functions of mitochondria finally lead to cell death, meaning that mitochondria coordinate the late stage of cellular demise. Pathological cell death induced by ischemia/reperfusion, intoxication with xenobiotics, neurodegenerative diseases, or viral infection also relies on MMP as a critical event. The inhibition of MMP constitutes an important strategy for the pharmaceutical prevention of unwarranted cell death. Conversely, induction of MMP in tumor cells constitutes the goal of anticancer chemotherapy.
Correlation of Vascular Endothelial Growth Factor Content With Recurrences, Survival, and First Relapse Site in Primary Node-Positive Breast Carcinoma After Adjuvant Treatment (2000)

The results suggest that VEGF(165) content in tumor cytosols is a predictor of RFS and OS in primary NPBC. VEGF content might also predict outcome after adjuvant endocrine treatment, but further studies in a prospective setting with homologous treatments are required.
Size at birth, maternal nutritional status in pregnancy, and blood pressure at age 17: population based analysis (1997)

Variables reflecting poor intrauterine nutrition, including low maternal body mass index before pregnancy, poor maternal weight gain in pregnancy, and being born small for gestational age, were not associated with a higher blood pressure in late adolescence.