gebhart/patrepeval huggingface dataset:
POTENTIAL HETEROTROPHY IN A NATURAL POPULATION OF OSCILLATORIA AGARDHII VAR. ISOTHRIX SKUJA1 (1972)

Direct evidence is presented from radioautography that four species of blue‐green algae can take up glucose‐ 14 C and acetate‐ 14 C at the low concentrations known to occur in lake water. The kinetics of glucose‐ 14 C uptake by Oscillatoria agardhii var. isothrix Skuja demonstrate that uptake is metabolic. The quantitative importance of such uptake in the development of the algal population cannot be assessed from the data, but the fact that O. agardhii develops massive densities in the microaerobic zone of many lakes suggests that heterotrophy may be very important to this alga and perhaps to other species occupying this habitat. The phenomenon may occur generally since O. agardhii and lakes with clinograde oxygen curves have worldwide distributions.
Initiation factor eIF-4E of Saccharomyces cerevisiae. Distribution within the cell, binding to mRNA, and consequences of its overproduction. (1994)

The eukaryotic translational initiation factor 4E (eIF-4E) is an essential protein that binds the 5' cap structure with high specificity and affinity. Yeast eIF-4E is homologous to eIF-4E of higher eukaryotes, but interacts with a different set of cap-binding complex proteins. In the present study the distribution of yeast eIF-4E in Saccharomyces cerevisiae was found to be similar to that observed in higher cells, whereby the yeast factor was more concentrated in the nucleus than in the cytoplasm. Overexpression of yeast eIF-4E in S. cerevisiae exerted at most a minimal effect on growth in liquid minimal medium and was not found to influence the translation of reporter gene mRNAs bearing secondary structure in their leader regions. In a new method to study mRNA-protein interactions, biotinylated mRNAs were synthesized in vitro for use in studies of the binding of eIF-4E in yeast extracts. Streptavidin was used to adsorb the biotinylated mRNAs plus bound initiation factors. Stem-loop structures in the leader region did not influence the binding of eIF-4E or, in comparative experiments, of eIF-4A. Thus yeast eIF-4E shows both similarities and differences with respect to the distribution and function of its counterparts in higher eukaryotes.
Probing Individual Molecules with Confocal Fluorescence Microscopy (1994)

Confocal fluorescence microscopy coupled with a diffraction-limited laser beam and a high-efficiency detection system has been used to study the diffusive movement and emission process of individual fluorescent molecules in the liquid phase at room temperature. The high detection sensitivity achieved at fast data acquisition speeds (greater than 1 kilohertz) allows real-time observation of single-molecule fluorescence without statistical analysis. The results show fluorescence-cycle saturation at the single-molecule level and multiple recrossings of a single molecule into and out of the probe volume as well as the triplet state.
Upgrading Petroleum Residues and Heavy Oils (1994)

"This useful reference offers in-depth coverage of current techniques for converting heavy oils and residues into more valuable distillates.Examines the chemistry of heavy hydrocarbon feeds and their properties important to engineering design, including phase behavior, reaction kinetics, and thermodynamic and transport characteristics!"
Interleukin 10 suppresses tumor growth and metastasis of human melanoma cells: potential inhibition of angiogenesis. (1996)

Interleukin 10 (IL-10) inhibits the production of a wide range of cytokines in various cell types. The purpose of this study was to determine whether the expression of the IL-10 gene can influence tumor growth and metastatic properties of human melanoma cells. The human melanoma cell line, A375P, which does not produce endogenous IL-10, was transfected with a hygromycin expression vector (control) or a vector containing full-length murine IL-10 cDNA. A375P parental cells, A375P-Hygro, and A375P-IL-10-positive cells were injected s.c. and i.v. into nude mice. A375P-IL-10 cells produced significantly slower growing s.c. tumors and fewer lung metastases than control cells. The tumorigenicity of the human melanoma A375SM and the murine melanoma B16-BL6 cells was also significantly inhibited when they were admixed with A375P-IL-10 but not with A375P-Hygro before s. c. injection into nude mice. The suppression of tumor growth and metastasis was directly correlated with a decrease in neovascularity determined by immunostaining with anti-factor VIII. Because tumor-associated macrophages are the major source of angiogenic molecules in melanoma, we used reverse transcription-PCR to demonstrate that IL-10 down-regulates the production of vascular endothelial growth factor, the most potent angiogenic factor in activated macrophages. Other factors involved in angiogenesis such as IL-1beta, tumor necrosis factor-alpha, IL-6, and the proteinase matrix metalloproteinase-9 were also inhibited in activated macrophages by supernatants from A375P-IL-10 cells. Collectively, these data suggest that the production of IL-10 by tumor cells inhibits macrophages-derived angiogenic factors, and hence, tumor growth and metastasis.
OncogenicCSF3RMutations in Chronic Neutrophilic Leukemia and Atypical CML (2013)

Mutations in CSF3R are common in patients with CNL or atypical CML and represent a potentially useful criterion for diagnosing these neoplasms. (Funded by the Leukemia and Lymphoma Society and others.).
Axicabtagene Ciloleucel CAR T-Cell Therapy in Refractory Large B-Cell Lymphoma (2017)

In this multicenter study, patients with refractory large B-cell lymphoma who received CAR T-cell therapy with axi-cel had high levels of durable response, with a safety profile that included myelosuppression, the cytokine release syndrome, and neurologic events. (Funded by Kite Pharma and the Leukemia and Lymphoma Society Therapy Acceleration Program; ZUMA-1 ClinicalTrials.gov number, NCT02348216 .).
High performance isoelectric focusing using capillary electrophoresis instrumentation. (1990)

High performance isoelectric focusing in capillaries provides rapid, high resolution separation of proteins based on their isoelectric points. Results can be obtained in a matter of minutes with little or no sample preparation. The technique requires the use of coated capillaries to reduce electroendosmosis so that stable, focused zones can be attained. Once focused, protein zones may be mobilized by the addition of salt to the catholyte or anolyte buffer. On-tube UV monitoring enables direct detection of sample components during mobilization with mass sensitivity equal to that of silver staining. The linear relationship between mobilization time and isoelectric point allows the technique to be used for estimation of protein pI. Demonstrated applications include separation of proteins in biological fluids with possible clinical applications, and characterization of biopharmaceutical proteins and monoclonal antibodies.
The I.M.A.G.E. Consortium: An Integrated Molecular Analysis of Genomes and Their Expression (1996)

We describe an effort to share resources such that the maximum amount of gene-related data is obtained with the last redundancy. Specifically, we describe the I.M.A.G.E. Consortium effort to share arrayed cDNA libraries, to have the data derived from the use of these common reagents placed in public databases, and to use these data to create master arrays containing a representative cDNA clone from each gene. 8 refs.
The second zinc atom in the matrix metalloproteinase catalytic domain is absent in the full‐length enzymes: a possible role for the C‐terminal domain (1995)

Domain deletion mutants of the matrix metalloproteinases consisting of the catalytic domain only contain two zinc atoms per molecule. One is essential for catalysis, while the other may fulfil a structural role. We have analysed the zinc contents of both the full-length and the truncated mutants of prostromelysin-1 and progelatinase A and report that the second zinc atom is not present in the full-length form of the proenzymes. Thus it seems likely that the role proposed for this zinc atom in maintaining the structure of the enzyme catalytic domain is performed by the C-terminal domain in the full-length enzyme.
Adenosine 5'-triphosphate dependent fluxes of manganese and hydrogen ions in sarcoplasmic reticulum vesicles (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAdenosine 5'-triphosphate dependent fluxes of manganese and hydrogen ions in sarcoplasmic reticulum vesiclesMichele Chiesi and Giuseppe InesiCite this: Biochemistry 1980, 19, 13, 2912–2918Publication Date (Print):June 24, 1980Publication History Published online1 May 2002Published inissue 24 June 1980https://pubs.acs.org/doi/10.1021/bi00554a015https://doi.org/10.1021/bi00554a015research-articleACS PublicationsRequest reuse permissionsArticle Views107Altmetric-Citations139LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Altered P2X7‐receptor level and function in mouse models of Huntington's disease and therapeutic efficacy of antagonist administration (2009)

The precise mechanism by which mutant huntingtin elicits its toxicity remains unknown. However, synaptic alterations and increased susceptibility to neuronal death are known contributors to Huntington's disease (HD) symptomatology. While decreased metabolism has long been associated with HD, recent findings have surprisingly demonstrated reduced neuronal apoptosis in Caenorhabditis elegans and Drosophila models of HD by drugs that diminish ATP production. Interestingly, extracellular ATP has been recently reported to elicit neuronal death through stimulation of P2X7 receptors. These are ATP-gated cation channels known to modulate neurotransmitter release from neuronal presynaptic terminals and to regulate cytokine production and release from microglia. We hypothesized that alteration in P2X7-mediated calcium permeability may contribute to HD synaptic dysfunction and increased neuronal apoptosis. Using mouse and cellular models of HD, we demonstrate increased P2X7-receptor level and altered P2X7-mediated calcium permeability in somata and terminals of HD neurons. Furthermore, cultured neurons expressing mutant huntingtin showed increased susceptibility to apoptosis triggered by P2X7-receptor stimulation. Finally, in vivo administration of the P2X7-antagonist Brilliant Blue-G (BBG) to HD mice prevented neuronal apoptosis and attenuated body weight loss and motor-coordination deficits. These in vivo data strongly suggest that altered P2X7-receptor level and function contribute to HD pathogenesis and highlight the therapeutic potential of P2X7 receptor antagonists.
Melanoma cell adhesion molecule identifies encephalitogenic T lymphocytes and promotes their recruitment to the central nervous system (2012)

In multiple sclerosis, encephalitogenic CD4(+) lymphocytes require adhesion molecules to accumulate into central nervous system inflammatory lesions. Using proteomic techniques, we identified expression of melanoma cell adhesion molecule (MCAM) on a subset of human effector memory CD4(+) lymphocytes and on human blood-brain barrier endothelium. Herein, we demonstrate that MCAM is a stable surface marker that refines the identification of interleukin 17(+), interleukin 22(+), RAR-related orphan receptor γ and interleukin 23 receptor(+) cells within the CD161(+)CCR6(+) subset of memory CD4(+) lymphocytes. We also show that MCAM(+) lymphocytes express significantly more granulocyte/macrophage colony stimulating factor and granzyme B than MCAM(-) lymphocytes. Furthermore, the proportion of MCAM(+) CD4(+) lymphocytes is significantly increased in the blood and in the central nervous system of patients with multiple sclerosis and experimental autoimmune encephalomyelitis animals compared with healthy controls or other neurological diseases, and MCAM expression is upregulated at the blood-brain barrier within inflammatory lesions. Moreover, blockade of MCAM or depletion of MCAM(+) CD4(+) T lymphocytes both restrict the migration of T(H)17 lymphocytes across blood-brain barrier endothelial cells and decrease the severity of experimental autoimmune encephalomyelitis. Our findings indicate that MCAM could serve as a potential biomarker for multiple sclerosis and represents a valuable target for the treatment of neuroinflammatory conditions.
A phase I study of two dosing schedules of volasertib (BI 6727), an intravenous polo-like kinase inhibitor, in patients with advanced solid malignancies (2014)

These data support further development of volasertib and a harmonised dosing for Asian and Caucasian patients.
Buspirone in Parkinsonʼs Disease (1986)

Buspirone, an anxiolytic unrelated to benzodiazepines that may act at the presynaptic dopamine receptor, was given to 11 patients with Parkinson's disease in an open label study. Seven patients completed the initial 10 week study achieving doses of 50-70 mg/day without any significant change in their clinical status. Six patients continued for an additional 3-11 weeks with increases in dose to 65-100 mg/day. Two of the three most severely affected patients had mild worsening of parkinsonian symptoms. Buspirone is ineffective in the treatment of Parkinson's disease, but at anxiolytic doses (less than 40 mg/day) does not adversely affect parkinsonian disability.
Synthesis of low-crest waveforms for multicarrier CDMA system (1995)

The crest factor of a waveform is the ratio of its peak power to its average power. A low crest factor increases the efficiency of the transmitter power amplifier. We study the problem of synthesizing signals with low crest factor for multi-carrier code division multiple access (MC-CDMA) systems. By using Golay (1961) complementary sequences as spreading codes, the crest factor is upper bounded by 6 dB regardless of the length of the spreading code. In comparison, the crest factor of DS-SS-CDMA pulses with certain chip shapes can grow unbounded. We also present methods for constructing a large number of pulses to accommodate multiple-access users.
Effects of amendments on biodegradation of crude petroleum by sediment bacteria from Bonny River Estuary (1999)

The biodegradation of Bonny light crude petroleum by bacteria in batch culture was enhanced by the addition to culture media, of 0.2 mg of urea and soya bean lecithin per 100 ml of crude oil, sediment and water mixture. Biodegradation was found to be purely an aerobic process. There was a direct relationship between hydrocarbon content and proportion (%) of total heterotrophic count that was capable of growing on crude petroleum as sole carbon and energy source.
In vitro characterization and delivery of chitosan-DNA microparticles into mammalian cells. (2004)

Chitosan microparticles provide a sustained release of plasmid DNA for a long period and they have a potential for DNA transfer into the mammalian cells. However, transfection efficiency of chitosan-DNA microparticles is low and dependent on the cell type.
Cefepime plus amikacin versus piperacillin-tazobactam plus amikacin for initial antibiotic therapy in haematology patients with febrile neutropenia: results of an open, randomized, multicentre trial (2002)

cefepime, piperacillin-tazobactam, amikacin, empirical antibiotic therapy, febrile neutropenia, haematological malignancy
Field-flattened, ring-like propagation modes (2013)

We present a method for designing optical fibers that support field-flattened, ring-like higher order modes, and show that the effective and group indices of its modes can be tuned by adjusting the widths of the guide's field-flattened layers or the average index of certain groups of layers. The approach provides a path to fibers that have simultaneously large mode areas and large separations between the propagation constants of their modes.
The ectodomain of the Notch3 receptor accumulates within the cerebrovasculature of CADASIL patients (2000)

Mutations in Notch3 cause CADASIL (cerebral autosomal dominant adult onset arteriopathy), which leads to stroke and dementia in humans. CADASIL arteriopathy is characterized by major alterations of vascular smooth muscle cells and the presence of specific granular osmiophilic deposits. Patients carry highly stereotyped mutations that lead to an odd number of cysteine residues within EGF-like repeats of the Notch3 receptor extracellular domain. Such mutations may alter the processing or the trafficking of this receptor, or may favor its oligomerization. In this study, we examined the Notch3 expression pattern in normal tissues and investigated the consequences of mutations on Notch3 expression in transfected cells and CADASIL brains. In normal tissues, Notch3 expression is restricted to vascular smooth muscle cells. Notch3 undergoes a proteolytic cleavage leading to a 210-kDa extracellular fragment and a 97-kDa intracellular fragment. In CADASIL brains, we found evidence of a dramatic and selective accumulation of the 210-kDa Notch3 cleavage product. Notch3 accumulates at the cytoplasmic membrane of vascular smooth muscle cells, in close vicinity to but not within the granular osmiophilic material. These results strongly suggest that CADASIL mutations specifically impair the clearance of the Notch3 ectodomain, but not the cytosolic domain, from the cell surface.
In vivo T helper cell response to retro-inverso peptidomimetics (1997)

Peptide analogues containing reversed peptide bonds between each residue along the peptide sequence (retro-inverso modification) have been analyzed for their antigenic and in vivo immunogenic properties in the MHC II and Th cell response context. Two antigenic peptides were selected for this study, namely peptide 103-115 of poliovirus VP1, which is involved in the production of Abs that neutralize the infectivity of the virus, and peptide 435-446 from the third constant region of mouse heavy chain IgG2a allopeptide gamma 2ab, which mimics a corneal Ag implicated in autoimmune keratitis. In a competition assay performed in vitro using reference hybridomas of known MHC class II restriction, both retro-inverso analogues bound (although more weakly in our test) to I-Ad and/or I-Ed class II molecules. However, in both cases, this lower affinity was apparently largely compensated in vivo, as a T cell response (with IL-2 secretion), equivalent to that obtained with the wild-type peptides, was observed following immunization of BALB/c mice with the retro-inverso analogues. Moreover, these T cells proliferated and produced IL-2 in response to the cognate peptides. It is concluded that the T cell receptors of T cells primed in vivo with the retro-inverso analogues readily cross-react with parent and retro-inverso analogue-MHC complexes. The approach of using pseudopeptides containing changes involving the backbone, and not the orientation of side chains, may thus be promising to design potent immunogens for class II-restricted T cells.
Experimental Ablation Study Using a New Long Linear Probe in Isolated Porcine Hearts. (1999)

We studied a new technique for creating long linear lesions in hearts using a custom-made linear probe. Radiofrequency (RF) energy applications using a 25-mm long stainless steel linear probe and a corresponding 500-kHz energy generator were tested, creating 90 lesions in isolated porcine hearts. The RF current was applied between the linear probe and a large patch electrode attached to the back of the specimen. Three parameters, comprising the power of the delivered energy, the pressure of contact between the probe and the specimen, and the duration of energy delivery were changed independently and the size of the resulting lesions was measured. All 90 lesions were transmural, well demarcated and created by a single stationary RF application. Lesion length and width increased with: 1) increasing power, when the other two parameters were maintained at constant levels, 2) increasing contact pressure, when the other two parameters were maintained at constant levels, and 3) increasing duration of energy delivery when the other two parameters were maintained at constant levels. The maximum width of the lesions was 3.7 mm. No overheating of any of the specimens was observed. In conclusion, the new original long linear probe used in this study was effective for creating transmural linear lesions, presenting the possibility of a worthwhile contribution to the maze surgical procedure applied to atrial fibrillation.
Extraction of 3D object features from CAD boundary representation using the super relation graph method (1995)

This paper presents the super relation graph (SRG) method for extracting prismatic features from the CAD boundary representation of a machined part. Using the definition of super relations and the validity of a feature volume, this method recognizes features with all three types of interactions: face splitting, face merging, and edge truncation.
360ProbDASH (2017)

Recently, there has been a significant interest towards 360-degree panorama video. However, such videos usually require extremely high bitrate which hinders their widely spread over the Internet. Tile-based viewport adaptive streaming is a promising way to deliver 360-degree video due to its on-request portion downloading. But it is not trivial for it to achieve good Quality of Experience (QoE) because Internet request-reply delay is usually much higher than motion-to-photon latency. In this paper, we leverage a probabilistic approach to pre-fetch tiles countering viewport prediction error, and design a QoE-driven viewport adaptation system, 360ProbDASH. It treats user's head movement as probability events, and constructs a probabilistic model to depict the distribution of viewport prediction error. A QoE-driven optimization framework is proposed to minimize total expected distortion of pre-fetched tiles. Besides, to smooth border effects of mixed-rate tiles, the spatial quality variance is also minimized. With the requirement of short-term viewport prediction under a small buffer, it applies a target-buffer-based rate adaptation algorithm to ensure continuous playback. We implement 360ProbDASH prototype and carry out extensive experiments on a simulation test-bed and real-world Internet with real user's head movement traces. The experimental results demonstrate that 360ProbDASH achieves at almost 39% gains on viewport PSNR, and 46% reduction on spatial quality variance against the existed viewport adaptation methods.