gebhart/patrepeval huggingface dataset:
Necrotic Death Pathway in FAS Receptor Signaling (2000)

A caspase 8-deficient subline (JB6) of human Jurkat cells can be killed by the oligomerization of Fas-associated protein with death domain (FADD). This cell death process is not accompanied by caspase activation, but by necrotic morphological changes. Here, we show that the death effector domain of FADD is responsible for the FADD-mediated necrotic pathway. This process was accompanied by a loss of mitochondrial transmembrane potential (DeltaPsim), but not by the release of cytochrome c from mitochondria. Pyrrolidine dithiocarbamate, a metal chelator and antioxidant, efficiently inhibited the FADD-induced reduction of DeltaPsim and necrotic cell death. When human Jurkat, or its transformants, expressing mouse Fas were treated with Fas ligand or anti-mouse Fas antibodies, the cells died, showing characteristics of apoptosis. A broad caspase inhibitor (z-VAD-fmk) blocked the apoptotic morphological changes and the release of cytochrome c. However, the cells still died, and this cell death process was accompanied by a strong reduction in DeltaPsim, as well as necrotic morphological changes. The presence of z-VAD-fmk and pyrrolidine dithiocarbamate together blocked cell death, suggesting that both apoptotic and necrotic pathways can be activated through the Fas death receptor.
Color Distribution ‐ A New Approach to Texture Compression (2000)

Texture compression is recently one of the most important topics of 3D scene rendering techniques, because it allows rendering more complicated high‐resolution scenes. However, because of some special requirements for these type of techniques, the commonly used block decomposition approach may introduce visual degradation of image details due to lack of colors. We present here a new approach to texture compression, which allows sharing of one color by several blocks providing a larger number of unique colors in each particular block and the best compression ratio. We also present an iterative algorithm for obtaining distributed colors on a texture, and discuss some advantages of our approach. The paper concludes with comparison of our technique with S3TC and other block decomposition methods.
Two-dimensional depth-resolved Mueller matrix characterization of biological tissue by optical coherence tomography (1999)

We built a polarization-sensitive optical coherence tomographic system and measured the two-dimensional depth-resolved full 4 x 4 Mueller matrix of biological tissue for what is believed to be the first time. The Mueller matrix measurements, which we made by varying the polarization states of the light source and the detector, yielded a complete characterization of the polarization property of the tissue sample. The initial experimental results indicated that this new approach reveals some tissue structures that are not perceptible in standard optical coherence tomography.
A Method for Measuring the Volume of Transdermally Extracted Interstitial Fluid by a Three-Electrode Skin Resistance Sensor (2014)

It is difficult to accurately measure the volume of transdermally extracted interstitial fluid (ISF), which is important for improving blood glucose prediction accuracy. Skin resistance, which is a good indicator of skin permeability, can be used to determine the volume of extracted ISF. However, it is a challenge to realize in vivo longitudinal skin resistance measurements of microareas. In this study, a three-electrode sensor was presented for measuring single-point skin resistance in vivo, and a method for determining the volume of transdermally extracted ISF using this sensor was proposed. Skin resistance was measured under static and dynamic conditions. The correlation between the skin resistance and the permeation rate of transdermally extracted ISF was proven. The volume of transdermally extracted ISF was determined using skin resistance. Factors affecting the volume prediction accuracy of transdermally extracted ISF were discussed. This method is expected to improve the accuracy of blood glucose prediction, and is of great significance for the clinical application of minimally invasive blood glucose measurement.
Alleles of the maize P gene with distinct tissue specificities encode Myb-homologous proteins with C-terminal replacements. (1996)

The maize P gene is a transcriptional regulator of genes encoding enzymes for flavonoid biosynthesis in the pathway leading to the production of a red phlobaphene pigment. Multiple alleles of the P gene confer distinct patterns of pigmentation to specific floral organs, such as the kernel pericarp and cob tissues. To determine the basis of allele-specific pigmentation, we have characterized the gene products and transcript accumulation patterns of the P-wr allele, which specifies colorless pericarps and red cob tissues. RNA transcripts of P-wr are present in colorless pericarps as well as in the colored cob tissues; however, the expression of P-wr in pericarp does not induce the accumulation of transcripts from the C2 and A1 genes, which encode enzymes for flavonoid pigment biosynthesis. The coding sequences of P-wr were compared with the P-rr allele, which specifies red pericarp and red cob. The P-wr and P-rr cDNA sequences are very similar in their 5' regions. There are only two nucleotide changes that result in amino acid differences; both are outside of the Myb-homologous DNA binding domain. In contrast, the 3' coding region of P-rr is replaced by a unique 210-bp sequence in P-wr. The predicted P-wr protein has a C-terminal sequence resembling a cysteine-containing metal binding domain that is not present in the P-rr protein. These results indicate that the differential pericarp pigmentation specified by the P-rr and P-wr alleles does not result from an absence of P-wr transcripts in pericarps. Rather, the allele-specific patterns of P-rr and P-wr pigmentation may be associated with structural differences in the proteins encoded by each allele.
Enhancement of antibody-dependent cytotoxicity with a chimeric anti-GD2 antibody. (1990)

A mouse/human chimeric antibody (ch14.18) was developed that reacts with the disialoganglioside GD2 on the surface of tumor cells of neuroectodermal origin. ch14.18 has the constant regions of a human IgG1 antibody and was expressed in a murine hybridoma. This antibody was produced in tissue culture at concentrations up to 180 mg/liter of spent culture fluid. ch14.18 was characterized and compared to 14.G2a, a murine mAb against GD2 of IgG2a isotype derived from the same parental hybridoma as ch14.18. Scatchard plot analysis of data from saturation binding studies on M21 melanoma cells showed identical binding for ch14.18 and 14.G2a. Indirect immunofluorescence revealed the same staining pattern for ch14.18 and 14.G2a on different melanoma cell lines. Both antibodies were equally capable of targeting M21 xenografts in athymic nude mice. ch14.18- and 14.G2a-activated human C-mediated cytolysis of melanoma cell; however, ch14.18-mediated antibody-dependent cytotoxicity of human effector cells against melanoma cells 50- to 100-fold more efficiently than 14.G2a.
Transdermal delivery of buprenorphine 3-alkyl-ester prodrugs for treatment of opioid dependence. (1995)

Improved pharmacotherapies for opioid dependence treatment have become a necessity for maintaining intravenous drug users in treatment and away from high-risk tuberculosis and HIV/AIDS transmission behavior. Buprenorphine (a synthetic partial agonist-antagonist opioid) has been clinically demonstrated to be effective in reducing illicit opioid use and maintaining patients in treatment at a high dose of eight milligrams per day given by sublingual tablet. To achieve the same dosage transdermally, prodrugs of buprenorphine have been synthesized and evaluated. Homologous 3-alkyl-ester prodrugs (C$\sb2$ to C$\sb7$) of buprenorphine have been characterized. Melting points and heats of fusion were determined by differential scanning calorimetry. Solubility parameters, which reflect the cohesion between like molecules, were calculated from thermal data and hexane solubilities. Aqueous solubilities, octanol/water partition coefficients, and chemical stability to hydrolysis were also determined for the prodrugs. Permeation of the prodrugs through human skin was determined in vitro. Prodrug metabolism was measured in human blood and skin supernatant in vitro. The low-melting lipophilic prodrugs have reduced crystallinity and raised solubilities in all but aqueous media as a result. On this basis one anticipates more total buprenorphine can be delivered from saturated solutions of the prodrugs than can be delivered from a saturated solution of buprenorphine. However, in no instance did the buprenorphine flux from the prodrug solutions through skin exceed the flux of buprenorphine base itself in vitro. Apparently the prodrugs' very high octanol/water partition coefficients (hydrophobicity) placed them in viable tissue layer controlled diffusion and one does not derive the benefit of reducing crystallinity.
Homeobox B3 Promotes Capillary Morphogenesis and Angiogenesis (2000)

Endothelial cells (EC) express several members of the Homeobox (Hox) gene family, suggesting a role for these morphoregulatory mediators during angiogenesis. We have previously established that Hox D3 is required for expression of integrin alphavbeta3 and urokinase plasminogen activator (uPA), which contribute to EC adhesion, invasion, and migration during angiogenesis. We now report that the paralogous gene, Hox B3, influences angiogenic behavior in a manner that is distinct from Hox D3. Antisense against Hox B3 impaired capillary morphogenesis of dermal microvascular EC cultured on basement membrane extracellular matrices. Although levels of Hox D3-dependent genes were maintained in these cells, levels of the ephrin A1 ligand were markedly attenuated. Capillary morphogenesis could be restored, however, by addition of recombinant ephrin A1/Fc fusion proteins. To test the impact of Hox B3 on angiogenesis in vivo, we constitutively expressed Hox B3 in the chick chorioallantoic membrane using avian retroviruses that resulted in an increase in vascular density and angiogenesis. Thus, while Hox D3 promotes the invasive or migratory behavior of EC, Hox B3 is required for the subsequent capillary morphogenesis of these new vascular sprouts and, together, these results support the hypothesis that paralogous Hox genes perform complementary functions within a particular tissue type.
Coherent population trapping in Raman-pulse atom interferometry (2011)

Raman pulse atom interferometry is an important modality for precision measurements of inertial forces and tests of fundamental physics. Typical Raman atom optics use two coherent laser fields applied at gigahertz-scale detunings from optical resonance, so that spontaneous emission produces a minor or negligible source of decoherence. An additional consequence of spontaneous emission is coherent population trapping (CPT). We show that CPT produces coherences and population differences which induce systematic effects in Raman pulse atom interferometers. We do not believe that CPT has been previously identified as an error mechanism in Raman pulse atom interferometry. We present an experimental characterization of CPT coherences and population differences induced in laser-cooled cesium atoms by application of Raman pulses at detunings near 1 GHz, commensurate with detunings used in several precision measurement experiments. We are not aware of previous demonstrations of CPT-induced population difference. We argue that CPT effects could induce phase shifts of several milliradians in magnitude for typical experimental parameters and stipulate that these errors can be suppressed by propagation direction reversal in Raman interferometer-based precision measurements.
Requirement of p53 targets in chemosensitization of colonic carcinoma to death ligand therapy (2003)

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) exhibits specific tumoricidal activity and is under development for cancer therapy. Mismatch-repair-deficient colonic tumors evade TRAIL-induced apoptosis through mutational inactivation of Bax, but chemotherapeutics including Camptosar (CPT-11) restore TRAIL sensitivity. However, the signaling pathways in restoring TRAIL sensitivity remain to be elucidated. Here, we imaged p53 transcriptional activity in Bax-/- carcinomas by using bioluminescence, in vivo, and find that p53 is required for sensitization to TRAIL by CPT-11. Small interfering RNAs directed at proapoptotic p53 targets reveal TRAIL receptor KILLER/DR5 contributes significantly to TRAIL sensitization, whereas Bak plays a minor role. Caspase 8 inhibition protects both CPT-11 pretreated wild-type and Bax-/- HCT116 cells from TRAIL-induced apoptosis, whereas caspase 9 inhibition only rescued the wild-type HCT116 cells from death induced by TRAIL. The results suggest a conversion in the apoptotic mechanism in HCT116 colon carcinoma from a type II pathway involving Bax and the mitochondria to a type I pathway involving efficient extrinsic pathway caspase activation. In contrast to Bax-/- cells, Bak-deficient human cancers undergo apoptosis in response to TRAIL or CPT-11, implying that these proteins have nonoverlapping functions. Our studies elucidate a mechanism for restoration of TRAIL sensitivity in MMR-deficient Bax-/- human cancers through p53-dependent activation of KILLER/DR5 and reconstitution of a type I death pathway. Efforts to identify agents that up-regulate DR5 may be useful in cancer therapies restoring TRAIL sensitivity.
Two Factor Reprogramming of Human Neural Stem Cells into Pluripotency (2009)

We propose that human NSCs represent an attractive source of cells for producing human iPS cells since they only require two factors, obviating the need for c-MYC, for induction into pluripotency. Thus, in vitro human disease models could be generated from iPS cells derived from human NSCs.
Review of robotic fixtures for minimally invasive surgery (2004)

Instrumental robotics aims to provide function-oriented solutions for given technical problems. Micro-Electro- Mechanical-Systems (MEMS) technologies provide miniaturized instruments, capable of effective duty-cycles at millimeter ranges. This review of current developments describes example devices, developed for intracorporeal operations; - system architectures, mainly concerned with problem-solving approaches and synthetic outlines of modified methods and layouts. This review is principally concerned with technologies, but the nature of the medical surroundings cannot be disregarded. Robotic devices serve surgical staff to help them accomplish surgical procedures. Safety and reliability require high functional and structural performance, especially for the active, autonomous stages of the operation. All these aspects are considered, and an overview is presented of characteristic features generally required for the miniaturization of instruments used in remote access surgery.
Collaborative document production using quilt (1988)

Quilt is a computer-based tool for collaborative document production. It provides annotation, messaging, computer conferencing, and notification facilities to support communication and information sharing among the collaborators on a document. Views of a document tailored to individual collaborators or to other of the document's users are provided by Quilt based on the user's position in a permission hierarchy that reflects an extensible set of social roles and communication types. This paper illustrates how Quilt could be used by collaborators to produce a document.
Fast Identification of Custom Instructions for Extensible Processors (2007)

This paper proposes a fast algorithm to enumerate all convex subgraphs that satisfy the I/O constraints from the dataflow graph (DFG) of a basic block. The algorithm can be tuned to determine all subgraphs or only those connected subgraphs. This allows a choice between better instruction-set extension (ISE) and faster design space exploration. The algorithm uses a grading method to identify the next node for inclusion into a subgraph. If the selected node is included, other related nodes are included as well, thus ensuring that the resultant subgraph is always convex and at the same time, reducing the problem size by a block of nodes. If the selected node is not included, the DFG will be split into smaller DFGs, thus reducing also the problem size. With this as base, the algorithm employs a simple but efficient method to prune the invalid subgraphs that violate the I/O constraints. Results show that for relatively small DFGs with small exploration space, the new algorithm has similar runtimes to that of existing algorithms. However, for larger DFGs with much larger exploration space and with multiple input and output constraints, the runtime improvement can be orders of magnitude better than that of existing algorithms. The new algorithm can be used to quickly identify custom instructions for ISE of embedded processors
A Null Mutation in Murine CD36 Reveals an Important Role in Fatty Acid and Lipoprotein Metabolism (1999)

A null mutation in the scavenger receptor gene CD36 was created in mice by targeted homologous recombination. These mice produced no detectable CD36 protein, were viable, and bred normally. A significant decrease in binding and uptake of oxidized low density lipoprotein was observed in peritoneal macrophages of null mice as compared with those from control mice. CD36 null animals had a significant increase in fasting levels of cholesterol, nonesterified free fatty acids, and triacylglycerol. The increase in cholesterol was mainly within the high density lipoprotein fraction, while the increase in triacylglycerol was within the very low density lipoprotein fraction. Null animals had lower fasting serum glucose levels when compared with wild type controls. Uptake of 3H-labeled oleate was significantly reduced in adipocytes from null mice. However, the decrease was limited to the low ratios of fatty acid:bovine serum albumin, suggesting that CD36 was necessary for the high affinity component of the uptake process. The data provide evidence for a functional role for CD36 in lipoprotein/fatty acid metabolism that was previously underappreciated.
Transthoracic monitoring of the impedance of the right lung in patients with cardiogenic pulmonary edema (2001)

The RS-205 is suitable for monitoring patients at high risk of CPE development. It enables detection of CPE and the monitoring of patients at all stages of CPE.
A Low Cost Terminal for the 1980's: Project Green Thumb (1980)

During this decade, low cost informatiom retrieval terminals (IRT's) will be available to consumers, professionals, and small businessmen. The mass marketing and distributiom of IRT's through retail outlets and the accompanying developement of an information service industry will make it possible for users to have access to vast information banks through existing communication facilities. Despite the obvious complexity of the regulatory enviornment in which the "information age is evolving, market acceptance, technological barriers, the energy crisis, a drive for increased productivity, and other socio-economic factors will encourage the development of information services and equipment. Teletext and videotext information services are already available in Great Britain, France, Germany, Canada and Japan. Many of these are in the test phase and are jointly sponsored by government and industry. In the U.S.A., the first federally funded test is a videotext style farm information retrieval system (FIRS), and is currently being tested in Kentucky. A description of this particular FIRS, Project Green Thumb, its implementation and its future significance is the subject of this dissertation.
Serial transplantation of methotrexate-resistant bone marrow: protection of murine recipients from drug toxicity by progeny of transduced stem cells (1990)

Recombinant retroviral vectors have been used to transfer a variety of genetic sequences into hematopoietic stem cells. Although transfer and expression of foreign genetic sequences into reconstituting stem cells is one approach to somatic gene therapy, few studies have shown long lasting phenotypic changes in recipient mice in vivo. In this study, we show successful transfer of a methotrexate-resistant cDNA (DHFRr) into reconstituting hematopoietic stem cells using a retroviral vector, FrDHFRr, in which the DHFR cDNA is expressed off a hybrid Friend/Moloney long term repeat. Both primary and secondary recipients transplanted with bone marrow cells infected with this recombinant retrovirus show improved survival and protection from methotrexate- induced marrow toxicity when compared with control animals. These data suggest that retroviral-mediated gene transfer of DHFRr cDNA leads to a stable change in the phenotype of hematopoietic stem cells and progeny derived from those cells in vivo after bone marrow transplantation. Gene transfer using recombinant retroviral vectors seems to be one rational approach to establishing chemotherapy-resistant bone marrow cells.
Synthesis and processing of the envelope gp55-116 complex of human cytomegalovirus (1986)

The envelope of human cytomegalovirus has been reported to contain between three and eight glycoproteins. Major constituents of the envelope include two abundant glycoproteins with estimated molecular weights of 55,000 (gp55) and 116,000 (gp116). These two glycoproteins have been shown to exist as a disulfide-linked complex (gp55-116) within the envelope of mature virions. Utilizing a panel of monoclonal antibodies reactive with the gp55-116 complex, we characterized the synthesis and processing of these two virion proteins. Infected cells were shown to contain two glycosylated proteins of 160,000 and 150,000 daltons as well as the mature gp55 and gp116. Pulse-chase analysis indicated that gp150 was a precursor protein of gp160. The mature gp55 and gp116 were generated, in turn, by cleavage of gp160. Antigenic and structural analysis revealed that gp55 and gp116 shared little structural homology and no detectable antigenic cross-reactivity. The results of this study are discussed in relation to the synthesis of envelope proteins of other herpesviruses.