gebhart/patrepeval huggingface dataset:
Power-Switching Converters (2005)

Significantly expanded and updated with extensive revisions, new material, and a new chapter on emerging applications of switching converters, Power-Switching Converters, Third Edition offers the same trusted, accessible, and comprehensive information as its bestselling predecessors. Similar to the two previous editions, this book can be used for an introductory as well as a more advanced course. Chapters begin with an introduction to switching converters and basic switching converter topologies. Entry level chapters continue with a discussion of resonant converters, isolated switching converters, and the control schemes of switching converters. Skipping to chapters 10 and 11, the subject matter involves an examination of interleaved converters and switched capacitor converters to round out and complete the overview of switching converter topologies. More detailed chapters include the continuous time-modeling and discrete-time modeling of switching converters as well as analog control and digital control. Advanced material covers tools for the simulation of switching converters (including both PSpice and Matlab simulations) and the basic concepts necessary to understand various actual and emerging applications for switching converters, such as power factor correction, LED drivers, low-noise converters, and switching converters topologies for solar and fuel cells. The final chapter contains several complete design examples, including experimental designs that may be used as technical references or for class laboratory projects. Supplementary information is available at crcpress.com including slides, PSpice examples (designed to run on the OrCAD 9.2 student version and PSIM software) and MATLAB scripts. Continuing the august tradition of its predecessors, Power-Switching Converters, Third Edition provides introductory and advanced information on all aspects of power switching converters to give students the solid foundation and applicable knowledge required to advance in this growing field.
The Zephyr Notification Service. (1988)

Zephyr is a notice transport and delivery system under development at Project Athena. 1 Zephyr is for use by network-based services and applications with a need for immediate, reliable and rapid communication with their clients. Zephyr meets the highthroughput, high fan-out communications requirements of large-scale workstation environments. It is designed as a suite of ‘‘layered services’ ’ based on a reliable, authenticated notice protocol. Multiple, redundant Zephyr servers provide basic routing, queueing, and dispatching services to clients that communicate via the Zephyr Client Library. More advanced communication services are built upon this base.
Production and Titering of Recombinant Adeno-associated Viral Vectors (2011)

In recent years recombinant adeno-associated viral vectors (AAV) have become increasingly valuable for in vivo studies in animals, and are also currently being tested in human clinical trials. Wild-type AAV is a non-pathogenic member of the parvoviridae family and inherently replication-deficient. The broad transduction profile, low immune response as well as the strong and persistent transgene expression achieved with these vectors has made them a popular and versatile tool for in vitro and in vivo gene delivery. rAAVs can be easily and cheaply produced in the laboratory and, based on their favourable safety profile, are generally given a low safety classification. Here, we describe a method for the production and titering of chimeric rAAVs containing the capsid proteins of both AAV1 and AAV2. The use of these so-called chimeric vectors combines the benefits of both parental serotypes such as high titres stocks (AAV1) and purification by affinity chromatography (AAV2). These AAV serotypes are the best studied of all AAV serotypes, and individually have a broad infectivity pattern. The chimeric vectors described here should have the infectious properties of AAV1 and AAV2 and can thus be expected to infect a large range of tissues, including neurons, skeletal muscle, pancreas, kidney among others. The method described here uses heparin column purification, a method believed to give a higher viral titer and cleaner viral preparation than other purification methods, such as centrifugation through a caesium chloride gradient. Additionally, we describe how these vectors can be quickly and easily titered to give accurate reading of the number of infectious particles produced.
Opportunistic Infections and Kaposi's Sarcoma Among Haitians: Evidence of a New Acquired Immunodeficiency State (1983)

Twenty Haitian patients, hospitalized from 1 April 1980 to 20 June 1982, had Pneumocystis carinii pneumonia, central nervous system toxoplasmosis, esophageal candidiasis, cryptococcosis, disseminated cytomegalovirus, progressive herpes simplex virus, chronic enteric coccidiosis, or invasive Kaposi's sarcoma. Ten patients died. Opportunistic infections were frequently multiple and were recurrent in three patients. In seven patients disseminated tuberculosis preceded the other infections by 2 to 15 months. There was no evidence of an underlying immunosuppressive disease, and no history of homosexuality or intravenous drug abuse. At least three patients probably acquired the syndrome in Haiti. Lymphadenopathy was common. Seventeen patients tested had anergy, and 18 had lymphopenia. Monoclonal antibody analysis of peripheral-blood T-cell subsets done on 11 patients showed a marked decrease in T-helper cells and an inversion of the normal ratio of T-helper cells to T-suppressor cells. This syndrome among heterosexual Haitians is strikingly similar to the syndrome of immunodeficiency described recently among American homosexuals.
Early identification of radiographic osteoarthritis of the hip using an active shape model to quantify changes in bone morphometric features: Can hip shape tell us anything about the progression of osteoarthritis? (2007)

Compared with traditional scoring methods, the ASM can be used more precisely to quantify the deforming effect of OA on the proximal femur and to identify, at an earlier stage of disease, those subjects at highest risk of developing radiographic OA or needing a THR. The ASM may therefore be useful as an imaging biomarker in the assessment of patients with hip OA.
Human Atrial Natriuretic Peptide Gene Delivery Reduces Blood Pressure in Hypertensive Rats (1995)

Chronic infusion of atrial natriuretic peptide (ANP) has been shown to cause natriuresis, diuresis, and hypotension in rats and humans. We explored the effect of a continuous supply of ANP by somatic ANP delivery on genetically hypertensive rats. A DNA construct containing the human ANP gene fused to the Rous sarcoma virus 3'-long terminal repeat (RSV-LTR) was injected intravenously into spontaneously hypertensive rats (SHR) through the tail vein. Expression of human ANP in SHR was identified in the heart, lung, and kidney by radioimmunoassay and reverse transcription-polymerase chain reaction followed by Southern blot analysis. A single injection of naked ANP plasmid DNA (12.3 kb) caused a significant reduction of systemic blood pressure in young SHR (4 weeks old), and the effect continued for 7 weeks. The differences were significant at 1 to 2 weeks (n = 6, P < .05) and 3 to 6 weeks after injection (n = 6, P < .01) A maximal blood pressure reduction of 21 mm Hg in young SHR was observed 5 weeks after injection with ANP DNA (159.4 +/- 3.02 mm Hg, mean +/- SEM, n = 6) compared with SHR injected with vector DNA alone (180.2 +/- 3.02 mm Hg, mean +/- SEM; n = 6; P < .01). Somatic gene delivery of human ANP DNA had no effect on the blood pressure of adult SHR (12 weeks old). After ANP gene delivery, there were significant increases in urinary volume and urinary potassium output (n = 6, P < .05) but not in body weight, heart rate, water intake, urinary sodium output, urinary creatine, and urinary protein.(ABSTRACT TRUNCATED AT 250 WORDS)
Flagellum ontogeny in trypanosomes studied via an inherited and regulated RNA interference system (2000)

The African trypanosome, Trypanosoma brucei possesses a large and unique intraflagellar structure called the paraflagellar rod (PFR). The PFR is composed of 2 major proteins, PFRA and PFRC. We have generated an inducible mutant trypanosome cell line (snl-2) that expresses linked inverted copies of a PFRA gene, capable of forming a PFRA double-stranded (ds) RNA. When expression of this dsRNA was induced, new PFRA RNA and PFRA protein quickly disappeared and PFR construction was affected, resulting in cell paralysis. This inducible RNA interference (RNAi) effect was fast-acting, heritable and reversible. It allowed us to demonstrate that PFR proteins are able to enter both mature and growing flagella but appear to concentrate differentially in new flagella because of the construction process. The PFR is constructed by a polar assembly process at the distal end of the flagellum resulting in a stable cytoskeletal structure with low turn-over. The inducible RNAi approach will have widespread applicability in studies of gene function and cellular processes in parasites.
Engineering Over-Clocking: Reliability-Performance Trade-Offs for High-Performance Register Files (2005)

Register files are in the critical path of most high-performance processors and their latency is one of the most important factors that limit their size. Our goal is to develop error correction mechanisms at the architecture level. Utilizing this increased robustness, the clock frequencies of the circuits are pushed beyond the point of allowing full voltage swing. This increases the errors observed due to noise and other external factors. The resulting errors are then corrected through the error correction mechanisms. We first develop a realistic model for error probability in register files for a given clock frequency. Then, we present the overall architecture, which allows the error detection computation to be overlapped with other computation in the pipeline. We develop novel techniques that utilize the fact that at a given instance many physical registers are not used in superscalar processors. These underutilized registers are used to store the values of active registers. Our simulation results show that for a fixed architecture the access times to the registers can be reduced by as much as 80% while increasing the number of execution cycles by 0.12%. On the other hand, by reducing the register file access pipeline stages by 75%, the average number of execution cycles of SPEC applications can be reduced by 11.5%.
Detection of the Cancer-Prone Colon, Using Transepithelial Impedance Analysis (1989)

Alterations in ionic conductance may represent an early mitogenic signal; therefore, impedance analysis was used to examine differences in the electrical properties of the distal colon in a cancer-susceptible (CF1) and a cancer-resistant (DBA) strain of mouse following administration of the carcinogen dimethylhydrazine. The electrical conductance of the surface colonic epithelium increased in CF1 mice from a mean (+/- SEM) of 41.1 +/- 3.0 milliSiemens.cm-2 (mS.cm-2) in controls to 52.6 +/- 3.1 mS.cm-2 following dimethylhydrazine treatment. The conductance decreased in the cancer-resistant DBA group from 154.6 +/- 44.1 mS.cm-2 in controls to 35.1 +/- 17.2 mS.cm-2 following dimethylhydrazine treatment. This difference in response to the carcinogen may partly explain differences in susceptibility noted between these two species. Epithelial impedance analysis may be of use in the early detection of the colon at risk for subsequent cancer development.
Universal PCR primers for detection of phytopathogenic Agrobacterium strains (1995)

Two PCR primer pairs, based on the virD2 and ipt genes, detected a wide variety of pathogenic Agrobacterium strains. The endonuclease domain of VirD2 protein, which cleaves transferred DNA (T-DNA) border sequences, is highly conserved; primer oligonucleotides specific for the endonuclease portion of virD2 detected all pathogenic strains of Agrobacterium tested. PCR primers corresponding to conserved sequences in ipt, the T-DNA-borne cytokinin synthesis gene, detected only Agrobacterium tumefaciens and distinguished it from Agrobacterium rhizogenes. The virD2 and ipt primer pairs did not interfere with each other when included in the same PCR amplification, and this permitted simultaneous detection of both genes in a single reaction. One nonpathogenic Agrobacterium radiobacter strain contained virD2 but not ipt; we speculate that this strain arose from a pathogenic progenitor through a deletion in the T-DNA. The virD2 primer pair appears to be universal for all pathogenic Agrobacterium species; used together, the primer sets reported here should allow unambiguous identification of Ti plasmid DNA in bacteria isolated from soil and plants.
Use of Personality Profile in Predicting Academic Emotion Based on Brainwaves Signals and Mouse Behavior (2011)

The academic emotion of learners is difficult to predict using EEG data, unless these brainwaves data undergo some extensive pre-processing operations. However, we show some evidence that it can be predicted somewhat more accurately for certain personality profiles. Twenty-five (25) college students were asked to use a math tutoring system while their brainwaves signals and mouse-click activities were being captured. Brainwaves signals were recorded using an Emotiv EEG device while the mouse behavior was based on the number of clicks, the duration of each click and the distance traveled by the mouse. The personality of the learners was evaluated based on the Big-Five Personality Test of Extroversion, Inquisitiveness, Accommodation, Emotional Stability and Orderliness. For each group based on personality type, the frequency of each self-reported academic emotion of confidence, excitement, frustration and interest was recorded and two classifiers, kNN and C4.5, were trained for each personality type. The accuracy rate of the classifiers built using only data instances from those assessed to be "low" in "orderliness", as well as only from those assessed to be "high" in "orderliness", performed significantly better compared to the classifiers that were trained for all personality types combined. The experiments also revealed that for almost all the 5 personality types, the percentage of instances where the learners reported themselves to be confident or frustrated differed significantly depending on whether they were assessed as "low" or "high" in the five personality types.
Speaker-Independent Speech-Driven Visual Speech Synthesis using Domain-Adapted Acoustic Models (2019)

Speech-driven visual speech synthesis involves mapping acoustic speech features to the corresponding lip animation controls for a face model. This mapping can take many forms, but a powerful approach is to use deep neural networks (DNNs). The lack of synchronized audio, video, and depth data is a limitation to reliably train DNNs, especially for speaker-independent models. In this paper, we investigate adapting an automatic speech recognition (ASR) acoustic model (AM) for the visual speech synthesis problem. We train the ASR-AM on ten thousand hours of audio-only transcribed speech. The ASR-AM is then adapted to the visual speech synthesis domain using ninety hours of synchronized audio-visual speech. Using a subjective assessment test, we compared the performance of the AM-initialized DNN to a randomly initialized model. The results show that viewers significantly prefer animations generated from the AM-initialized DNN than the ones generated using the randomly initialized model. We conclude that visual speech synthesis can significantly benefit from the powerful representation of speech in the ASR acoustic models.
Common and Selective Molecular Determinants Involved in Metabotopic Glutamate Receptor Agonist Activity (2002)

Several potent and group selective agonists of metabotropic glutamate receptors (mGluRs) have been docked at mGlu1,2,4R binding sites in the closed conformation of the bilobate extracellular domain. Quisqualic acid and (S)-3,5-dihydroxyphenylglycine (3,5-DHPG) were selected for mGlu1R, dicarboxycyclopropylglycine (DCG-IV), LY354740, (S)-4-carboxyphenylglycine (4CPG) for mGlu2R, and (S)-2-amino-4-phosphonobutyric acid (AP4), 1-aminocyclopentane-1,3,4-tricarboxylic acid (ACPT-I), (S)-4-phosphonophenylglycine (PPG) for mGlu4R. The models show a conserved binding pattern for the glycine moiety (alpha-amino and alpha-acidic functions) and group specific bindings for the distal acidic function. The best agonists allow optimized interaction with both lobes of the binding domain. Interlobe connections around the ligand are also described and participate in stabilizing the closed form of the amino-terminal domain. Altogether, the docking models support the proposal that the stabilization of a closed state represents a key step in agonist activation of mGluRs.
P-Glycoprotein Antibody Functionalized Carbon Nanotube Overcomes the Multidrug Resistance of Human Leukemia Cells (2010)

Multidrug resistance (MDR), which is related to cancer chemotherapy, tumor stem cells, and tumor metastasis, is a huge obstacle for the effective cancer therapy. One of the underlying mechanisms of MDR is the increased efflux of anticancer drugs by overexpressed P-glycoprotein (P-gp) of multidrug resistant cells. In this work, the antibody of P-gp (anti-P-gp) functionalized water-soluble single-walled carbon nanotubes (Ap-SWNTs) loaded with doxorubicin (Dox), Dox/Ap-SWNTs, were synthesized for challenging the MDR of K562 human leukemia cells. The resulting Ap-SWNTs could not only specifically recognize the multidrug resistant human leukemia cells (K562R), but also demonstrate the effective loading and controllable release performance for Dox toward the target K562R cells by exposing to near-infrared radiation (NIR). The recognition capability of Ap-SWNTs toward the K562R cells was confirmed by flow cytometry (FCM) and confocal laser scanning microscopy (CLSM). The binding affinity of Ap-SWNTs toward drug-resistant K562R cells was ca. 23-fold higher than that toward drug-sensitive K562S cells. Additionally, CLSM indicated that Ap-SWNTs could specifically localize on the cell membrane of K562R cells and the fluorescence of Dox in K562R cells could be significantly enhanced after the employment of Ap-SWNTs as carrier. Moreover, the composite of Dox and Ap-SWNTs (Dox/Ap-SWNTs) expressed 2.4-fold higher cytotoxicity and showed the significant cell proliferation suppression toward K562R leukemia cells (p < 0.05) as compared with free Dox which is popularly employed in clinic trials. These results suggest that the Ap-SWNTs are the promising drug delivery vehicle for overcoming the MDR induced by the overexpression of P-gp on cell membrane. Ap-SWNTs loaded with drug molecules could be used to suppress the proliferation of multidrug resistant cells, destroy the tumor stem cells, and inhibit the metastasis of tumor.
The naive repertoire of human T helper cells specific for gp120, the envelope glycoprotein of HIV (1991)

The envelope glycoprotein of HIV gp120 is a T cell Ag in experimental animals and in humans infected with HIV or deliberately immunized with gp120 in various forms. Inasmuch as T cell responses result from the interaction of Ag processed and presented by APC with the unprimed T cell repertoire, we have investigated the human T cell repertoire specific for gp120 in seronegative, normal individuals. T cell lines and clones specific for HIV gp120 were generated by repeated in vitro stimulation of peripheral blood T lymphocytes with gp120-pulsed APC, followed by IL-2 expansion. We observed that the T cell response to whole gp120 involved single restricted immunodominant epitopes in gp120 that differ between responding individuals. Focusing of the response to limited regions of gp120 when the whole Ag is used for priming suggests that one or more adjacent epitopes are immunodominant and mask responses to "immunorecessive" epitopes. We have been able to generate primary in vitro responses to recessive epitopes by stimulation in vitro with synthetic peptides of gp120. The results indicate that a much broader T repertoire can be detected when individual peptides are used for priming in vitro rather than gp120. This information has important implications for the development of vaccination protocols aimed at eliciting diverse immune responses to "immunorecessive" regions of envelope glycoprotein.
Adsorption of monovalent cations to bilayer membranes containing negative phospholipids (1979)

The electrophoretic mobilities of multilamellar phosphatidylserine vesicles were measured in solutions containing monovalent cations, and the xi potentials, the electrostatic potentials at the hydrodynamic plane of shear, were calculated from the Helmholtz--Smoluchowski equation. In the presence of 0.1 M lithium, sodium, ammonium, potassium, rubidium, cesium, tetraethylammonium, and tetramethylammonium chloride, the xi potentials were -60, -62, -72, -73, -77, -80, -82, and -91 mV, respectively. Similar results were obtained with phosphatidylglycerol vesicles; different results were obtained with cardiolipin, phosphatidylinositol, and phosphatidic acid vesicles. The phosphatidylserine results are interpreted in terms of the Stern equation, a combination of the Gouy equation from the theory of the diffuse double layer, the Boltzmann relation, and the Langmuir adsorption isotherm. Evidence is presented that suggests the hydrodynamic plane of shear is 2 A from the surface of the membrane in solutions containing the alkali metal cations. With this assumption, the intrinsic association constants of the above monovalent cations with phosphatidylserine are 0.8, 0.6, 0.17, 0.15, 0.08, 0.05, 0.03, and 0 M-1, respectively. The validity of this approach was tested in two ways. First, the xi potentials of vesicles formed from mixtures of phosphatidylserine and a zwitterionic lipid, phosphatidylcholine, were measured in solutions containing different concentrations of sodium. All the data could be described by the Stern equation if the "relaxation" of the ionic atmosphere, which is predicted by classic electrostatic and hydrodynamic theory to occur at low salt concentrations and high potentials, was circumvented by using only large (diameter greater than 13 micrometers) vesicles for these measurements. Second, the fluorescent probe 2-(p-toluidinyl)naphthalene-6-sulfonate was used to estimate the potential at the surface of phosphatidylserine and phosphatidylglycerol vesicles sonicated in 0.1 M NaCl. Reasonable agreement with the predicted values of the surface potential was obtained.