gebhart/patrepeval huggingface dataset:
Simulated Seizures and Spreading Depression in a Neuron Model Incorporating Interstitial Space and Ion Concentrations (2000)

Sustained inward currents in neuronal membranes underlie tonic-clonic seizure discharges and spreading depression (SD). It is not known whether these currents flow through abnormally operating physiological ion channels or through pathological pathways that are not normally present. We have now used the NEURON simulating environment of Hines, Moore, and Carnevale to model seizure discharges and SD. The geometry and electrotonic properties of the model neuron conformed to a hippocampal pyramidal cell. Voltage-controlled transient and persistent sodium currents (I(Na,T) and I(Na,P)), potassium currents (I(K,DR) and I(K,A)), and N-methyl-D-aspartate (NMDA) receptor-controlled currents (I(NMDA)), were inserted in the appropriate regions of the model cell. The neuron was surrounded by an interstitial space where extracellular potassium and sodium concentration ([K(+)](o) and [Na(+)](o)) could rise or fall. Changes in intra- and extracellular ion concentrations and the resulting shifts in the driving force for ionic currents were continuously computed based on the amount of current flowing through the membrane. A Na-K exchange pump operated to restore ion balances. In addition, extracellular potassium concentration, [K(+)](o), was also controlled by a "glial" uptake function. Parameters were chosen to resemble experimental data. As long as [K(+)](o) was kept within limits by the activity of the Na-K pump and the "glial" uptake, a depolarizing current pulse applied to the cell soma evoked repetitive firing that ceased when the stimulating current stopped. If, however, [K(+)](o) was allowed to rise, then a brief pulse provoked firing that outlasted the stimulus. At the termination of such a burst, the cell hyperpolarized and then slowly depolarized and another burst erupted without outside intervention. Such "clonic" bursting could continue indefinitely maintained by an interplay of the rise and fall of potassium and sodium concentrations with membrane currents and threshold levels. SD-like depolarization could be produced in two ways, 1) by a dendritic NMDA-controlled current. Glutamate was assumed to be released in response to rising [K(+)](o). And 2) by the persistent (i.e., slowly inactivating) Na-current, I(Na,P). When both I(NMDA) and I(Na,P) were present, the two acted synergistically. We conclude that epileptiform neuronal behavior and SD-like depolarization can be generated by the feedback of ion currents that change ion concentrations, which, in turn, influence ion currents and membrane potentials. The normal stability of brain function must depend on the efficient control of ion activities, especially that of [K(+)](o).
Tumor cell motility. (1991)

Tumor cell motility is required for invasion and metastasis. The locomotory machinery of the cell includes cell projections called pseudopodia which are regulated by a complicated linkage between cell surface receptors or sensors and the internal cytoskeleton. Recently a new class of motility stimulating cytokines have been identified. These cytokines can function as autocrine motility factors and require a pertussis toxin sensitive G protein pathway to transduce a random motile response.
Adenovirus-Mediated Interferon- γ Transfer Inhibits Growth of Transplanted HTLV-1 Tax Tumors in Mice (1996)

Human T cell leukemia virus type 1 (HTLV-1) causes adult T cell leukemia (ATL), and the virus-encoded trans-activator, Tax, plays an important role in T cell transformation. In the HTLV-1 long terminal repeat (LTR)-Tax transgenic mouse model, Tax expression causes fibroblastic tumors. A tumor-derived cell line (B line) obtained from an explant of a Tax-transformed tumor, was established. This line expresses high levels of many cytokines as a consequence of Tax activation. However, the tumors are not immunogenic when transplanted into syngeneic mice. Because B line cells do not express the immunogenic cytokine interferon-gamma (IFN-gamma), a replication-defective adenoviral vector was used to deliver the IFN-gamma gene to tumor cells. The recombinant IFN-gamma adenovirus (IFN-gamma/Ad) can efficiently infect B line cells, resulting in high levels of IFN-gamma expression and secretion. Local secretion of IFN-gamma from B line cells caused both CD(4+)- and CD(8+)-positive T cell infiltration, and completely inhibited local tumor development in transplanted mice. Immunization with these cells significantly delayed tumor development after subsequent challenges of parental tumor cells. Expression of IFN-gamma in B cells also partially inhibited the highly expressed immune suppressive cytokine, transforming growth factor-beta 1 (TGF-beta 1). This system provides us with a valuable tumor immune therapy model to evaluate the effects of cytokines in induction or inhibition of specific antitumor immunity.
Isolation of a receptor protein involved in attachment of human rhinoviruses (1986)

Human rhinoviruses can be classified into major and minor groups on the basis of receptor specificity. Recently, a mouse monoclonal antibody was isolated which selectively blocked the attachment of the major group of human rhinoviruses to cells. Using this monoclonal antibody, the cellular receptor for the major group of human rhinoviruses was isolated. A radioimmunoassay was developed by using the receptor antibody to specifically detect rhinovirus receptor during isolation. Solubilized receptor from detergent-treated HeLa cell membrane extracts eluted from gel filtration columns with an apparent molecular weight of 440,000. A cellular receptor protein, which had a molecular weight of 90,000 when analyzed on sodium dodecyl sulfate-polyacrylamide gels, was purified from solubilized extracts on an immunoaffinity column containing receptor antibody. Polyclonal rabbit antiserum, resulting from immunization with the isolated receptor protein, specifically blocked the attachment of the major group of human rhinoviruses and indicated that the 90-kilodalton protein plays a functional role in attachment. Prolonged exposure of HeLa cell monolayers with the receptor antibody showed no inhibition of cell growth and division.
Isometric Occupational Exercise and the Incidence of Hypertension (1985)

The effect on blood pressure of regular exposure to isometric exercise was studied by measuring the incidence of hypertension among 4,273 men classified according to occupational isometric activity. Incidence of hypertension was lower among men in jobs with moderate or heavy isometric activity. This difference persisted after statistical adjustment for age, social class, obesity, and use of alcohol.
Plant Cyclopeptides (2006)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPlant CyclopeptidesNing-Hua Tan and Jun ZhouView Author Information State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 650204 Kunming, Yunnan, People's Republic of China Cite this: Chem. Rev. 2006, 106, 3, 840–895Publication Date (Web):February 14, 2006Publication History Received7 July 2005Published online14 February 2006Published inissue 1 March 2006https://pubs.acs.org/doi/10.1021/cr040699hhttps://doi.org/10.1021/cr040699hresearch-articleACS PublicationsCopyright © 2006 American Chemical SocietyRequest reuse permissionsArticle Views6387Altmetric-Citations325LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Conformation,Monomers,Organic compounds,Peptides and proteins,Plants Get e-Alerts
ChemInform Abstract: AMINOCYCLOPROPENIUM‐ION (1971)

Abstract Das Cyclopropen (I) liefert mit dem Amin (IIa) bei nachfolgender Behandlung mit Perchlorsäure quantitativ das Cyclopropenyliumperchlorat (IIIa).
Equation of State Calculations by Fast Computing Machines (1953)

A general method, suitable for fast computing machines, for investigating such properties as equations of state for substances consisting of interacting individual molecules is described. The method consists of a modified Monte Carlo integration over configuration space. Results for the two-dimensional rigid-sphere system have been obtained on the Los Alamos MANIAC and are presented here. These results are compared to the free volume equation of state and to a four-term virial coefficient expansion.
The biorheology of tumor cells (1984)

A series of in vitro experiments testing the ability of tumor cells to withstand shear stresses typical of the circulation were carried out. Shearing suspensions of B16 murine melanoma cells in either a cone-plate viscometer or a tubing circuit caused loss of viability whose rate increased with increasing shear rate and shear stress. The killing rate did not depend on the ratio of the container area to volume of the viscometers, suggesting that interactions with the bounding surfaces were not responsible for the cell damage. The loss of viability was not due to the action of intracellular products released during shearing. The results show that all the tumor cells examined were considerably more sensitive to shear than any of the blood cellular elements. Fluid shear stresses in the physiological range can kill tumor cells over time intervals of the order of minutes to hours, results very similar to the time courses observed for tumor cell destruction following injection into the circulation in animal models of metastasis.
Purification, toxicity, and antiendotoxin activity of polymyxin B nonapeptide (1989)

Polymyxin B, a relatively toxic antibiotic, has potent endotoxin-neutralizing properties that may be beneficial as adjunctive therapy in gram-negative sepsis. Polymyxin B nonapeptide (deacylated polymyxin B) is devoid of antibiotic activity but retains the capacity to disorganize the outer membrane of gram-negative bacteria. To evaluate the potential therapeutic usefulness of this derivative, we produced purified polymyxin B nonapeptide, tested its in vivo toxicity in animals, and evaluated its in vitro antiendotoxin activity. Effectiveness as an antiendotoxin agent was assessed by examining the ability of polymyxin B nonapeptide to block the enhanced release of toxic oxygen radicals induced by lipopolysaccharide in human neutrophils (priming). In vivo, at doses of 1.5 and 3.0 mg/kg, polymyxin B nonapeptide did not exhibit the neuromuscular blocking, neurotoxic, or nephrotoxic effects that were observed with polymyxin B sulfate. Both polymyxin B and polymyxin B nonapeptide inhibited lipopolysaccharide-induced neutrophil priming in a concentration-dependent manner, but the parent compound, polymyxin B, was 63 times more effective on a weight basis. The inhibitory activity of both compounds, however, diminished rapidly when they were added after the start of the lipopolysaccharide-neutrophil incubation. We conclude that polymyxin B nonapeptide is less toxic than polymyxin B and, at the doses tested, lacks the neurotoxicity and nephrotoxicity of the parent compound. Polymyxin B nonapeptide retains the antiendotoxin activity of polymyxin B but is much less potent. The findings suggest that these compounds block an early step in the neutrophil priming process, possibly lipopolysaccharide attachment to or insertion into the neutrophil membrane.
The humanID gait challenge problem: data sets, performance, and analysis (2005)

Identification of people by analysis of gait patterns extracted from video has recently become a popular research problem. However, the conditions under which the problem is "solvable" are not understood or characterized. To provide a means for measuring progress and characterizing the properties of gait recognition, we introduce the HumanID Gait Challenge Problem. The challenge problem consists of a baseline algorithm, a set of 12 experiments, and a large data set. The baseline algorithm estimates silhouettes by background subtraction and performs recognition by temporal correlation of silhouettes. The 12 experiments are of increasing difficulty, as measured by the baseline algorithm, and examine the effects of five covariates on performance. The covariates are: change in viewing angle, change in shoe type, change in walking surface, carrying or not carrying a briefcase, and elapsed time between sequences being compared. Identification rates for the 12 experiments range from 78 percent on the easiest experiment to 3 percent on the hardest. All five covariates had statistically significant effects on performance, with walking surface and time difference having the greatest impact. The data set consists of 1,870 sequences from 122 subjects spanning five covariates (1.2 Gigabytes of data). The gait data, the source code of the baseline algorithm, and scripts to run, score, and analyze the challenge experiments are available at http://www.GaitChallenge.org. This infrastructure supports further development of gait recognition algorithms and additional experiments to understand the strengths and weaknesses of new algorithms. The more detailed the experimental results presented, the more detailed is the possible meta-analysis and greater is the understanding. It is this potential from the adoption of this challenge problem that represents a radical departure from traditional computer vision research methodology.
Isolation of Listeria monocytogenes mutants with high‐level in vitro expression of host cytosol‐induced gene products (2003)

The facultative intracellular bacterial pathogen Listeria monocytogenes dramatically increases the expression of several key virulence factors upon entry into the host cell cytosol. actA, the protein product of which is required for cell-to-cell spread of the bacterium, is expressed at low to undetectable levels in vitro and increases in expression more than 200-fold after L. monocytogenes escape from the phagosome. To identify bacterial factors that participate in the intracellular induction of actA expression, L. monocytogenes mutants expressing high levels of actA during in vitro growth were selected after chemical mutagenesis. The resulting mutant isolates displayed a wide range of actA expression levels, and many were less sensitive to environmental signals that normally mediate repression of virulence gene expression. Several isolates contained mutations affecting actA gene expression that mapped at least 40 kb outside the PrfA regulon, supporting the existence of additional regulatory factors that contribute to virulence gene expression. Two actA in vitro expression mutants contained novel mutations within PrfA, a key regulator of L. monocytogenes virulence gene expression. PrfA E77K and PrfA G155S mutations resulted in high-level expression of PrfA-dependent genes, increased bacterial invasion of epithelial cells and increased virulence in mice. Both prfA mutant strains were significantly less motile than wild-type L. monocytogenes. These results suggest that, although constitutive activation of PrfA and PrfA-dependent gene expression may enhance L. monocytogenes virulence, it may conversely hamper the bacterium's ability to compete in environments outside host cells.
Assays of the minimal residual disease in pediatric acute leukemia (2011)

Minimal residual disease(MRD)iS the origin of relapse in leukemia.So it's significant to quantitate MRD in clinical.At present, there are three MRD quantitative assays, such as flow cytometric detection of abnormal immuno-phenotype,polymerase chain reaction(PCR)amplification of antigenreceptor genes and fusion transcripts.Because every measure has its own principle and feature.the clinical applications are different.Selecting suitable assays based on the characteristic of patients can help to quantitate MRD exactly.

Key words: 
Acute leukemia; Minimal residual disease; Flow cytometry; Polymerase chain reaction
Deletions in the Second Stalk of F1F0-ATP Synthase in Escherichia coli (1998)

In Escherichia coli F1F0-ATP synthase, the two b subunits form the second stalk spanning the distance between the membrane F0 sector and the bulk of F1. Current models predict that the stator should be relatively rigid and engaged in contact with F1 at fixed points. To test this hypothesis, we constructed a series of deletion mutations in the uncF(b) gene to remove segments from the middle of the second stalk of the subunit. Mutants with deletions of 7 amino acids were essentially normal, and those with deletions of up to 11 amino acids retained considerable activity. Membranes prepared from these strains had readily detectable levels of F1-ATPase activity and proton pumping activity. Removal of 12 or more amino acids resulted in loss of oxidative phosphorylation. Levels of membrane-associated F1-ATPase dropped precipitously for the longer deletions, and immunoblot analysis indicated that reductions in activity correlated with reduced levels of b subunit in the membranes. Assuming the likely alpha-helical conformation for this area of the b subunit, the 11-amino acid deletion would result in shortening the subunit by approximately 16 A. Since these deletions did not prevent the b subunit from participating in productive interactions with F1, we suggest that the b subunit is not a rigid rodlike structure, but has an inherent flexibility compatible with a dynamic role in coupling.
Dirty paper coding vs. TDMA for MIMO broadcast channels (2004)

In this paper we derive an upper bound on the sum-rate gain that dirty-paper coding provides over TDMA for MIMO broadcast channels. We find that the sum-rate capacity (achievable using dirty-paper coding) of the multiple-antenna broadcast channel is at most min(M;K) times the largest single-user capacity (i.e. the TDMA sum-rate) in the system, where M is the number of transmit antennas and K is the number of receivers. This result is independent of the number of receive antennas. We investigate the tightness of this bound in a time-varying channel (assuming perfect channel knowledge at receivers and transmitters) where the channel experiences uncorrelated Rayleigh fading and in some situations we find that the dirty paper gain is upper bounded by the ratio of transmit to receive antennas. We also show that min(M,K) upper bounds the sum rate gain of successive decoding over TDMA for the uplink, where M is the number of receive antennas at the base station and K is the number of transmitters.
10‐3: Invited Paper : Organic Vapor Jet Printing, a Solvent‐Less, Mask‐Less Patterning Technology for OLED Displays (2017)

Organic Vapor Jet Printing (OVJP) is a solvent‐less, mask‐less printing technology for depositing patterned small molecule organic materials to manufacture large area side by side R‐G‐B OLED displays. This paper discusses a brief history of OVJP, describes recent technology advances and describes prospects for large area mass production systems.
Activation of Chaperone-mediated Autophagy during Oxidative Stress (2004)

Oxidatively damaged proteins accumulate with age in almost all cell types and tissues. The activity of chaperone-mediated autophagy (CMA), a selective pathway for the degradation of cytosolic proteins in lysosomes, decreases with age. We have analyzed the possible participation of CMA in the removal of oxidized proteins in rat liver and cultured mouse fibroblasts. Added to the fact that CMA substrates, when oxidized, are more efficiently internalized into lysosomes, we have found a constitutive activation of CMA during oxidative stress. Oxidation-induced activation of CMA correlates with higher levels of several components of the lysosomal translocation complex, but in particular of the lumenal chaperone, required for substrate uptake, and of the lysosomal membrane protein (lamp) type 2a, previously identified as a receptor for this pathway. In contrast with the well characterized mechanism of CMA activation during nutritional stress, which does not require de novo synthesis of the receptor, oxidation-induced activation of CMA is attained through transcriptional up-regulation of lamp2a. We conclude that CMA is activated during oxidative stress and that the higher activity of this pathway under these conditions, along with the higher susceptibility of the oxidized proteins to be taken up by lysosomes, both contribute to the efficient removal of oxidized proteins.
GaN nanowire lasers with low lasing thresholds (2005)

We report optically pumped room-temperature lasing in GaN nanowires grown by metalorganic chemical vapor deposition (MOCVD). Electron microscopy images reveal that the nanowires grow along a nonpolar ⟨11-20⟩ direction, have single-crystal structures and triangular cross sections. The nanowires function as free-standing Fabry–Pérot cavities with cavity mode spacings that depend inversely on length. Optical excitation studies demonstrate thresholds for stimulated emission of 22kW∕cm2 that are substantially lower than other previously reported GaN nanowires. Key contributions to low threshold lasing in these MOCVD GaN nanowire cavities and the development of electrically pumped GaN nanowire lasers are discussed.
Heat stress proteins and experimental cancer metastasis (1986)

The influence of stress protein synthesis and thermotolerance on blood-borne (experimental) metastatic potential was examined in the 13762NF rat mammary adenocarcinoma model. Cloned cell populations with highly reproducible and well-defined metastatic potential were treated by hyperthermia and sodium arsenite to induce a complete set of stress proteins and thermotolerance with minimal cell killing. The influence of these in vitro treatments on subsequent experimental metastasis in vivo was determined for location, frequency, size distribution and volume. Metastatic tumour burden generally decreased following induction of increased heat or arsenite stress proteins and thermotolerance; however, there was no evidence for altered size distribution or location of metastatic lesions.
Inositol Phosphate Phosphatases of Microbiological Origin. The Isolation of Soil Bacteria Having Inositol Phosphate Phosphatase Activity (1970)

The isolation of three soil bacteria exhibiting inositol phosphate phosphatase activity is described. One isolate, a member of the genus Pseudomonas, had pronounced phytase activity but the others had not. The latter were slightly active against pentaphosphates, however, and they easily dephosphorylated tetraphosphates and lower esters.
Collagen type IV ( 3- 4) nephropathy: from isolated haematuria to renal failure (2004)

Recent evidence has shown that theCOL4A3,COL4A4 and COL4A5 genes are involved in different renal manifestations. Mutations in these collagen type IV genes affect the glomerular basement membrane (GBM) giving rise to a nephropathy whose symptoms range from isolated haematuria to severe renal failure. This disorder has been traditionally considered to be different entities, but the increased knowledge of the molecular basis of this clinical diversity prompted us to agglutinate these entities under the name of ‘collagen type IV nephropathy’. This fact has relevant implications in diagnosis, prognosis and management.