gebhart/patrepeval huggingface dataset:
Structural analysis of the sequence coding for an inducible 26‐kDa protein in human fibroblasts (1986)

When human fibroblast cells were stimulated with poly(I) X poly(C) in the presence of cycloheximide for the production of interferon-beta (IFN-beta), a 26-kDa protein could be immunoprecipitated by antiserum raised against partially purified human IFN-beta [Content, J., De Wit, L., Pierard, D., Derynck, R., De Clercq, E. & Fiers, W. (1982) Proc. Natl Acad. Sci. USA 79, 2768-2772]. In our hands this 26-kDa protein showed no antiviral activity. Other investigators have, however, reported the presence in the same conditions of a second type of IFN, a so-called beta 2 species [Weissenbach, J., Chernajovsky, Y., Zeevi, M., Shulman, L., Soreq, H., Nir, U., Wallach, D., Perricaudet, M., Tiollais, P. & Revel, M. (1980) Proc. Natl Acad. Sci. USA 77, 7152-7156] of which the mRNA structure and protein characteristics strongly suggests identity with the 26-kDa product. In this paper we describe the nucleotide sequence of the 26-kDa cDNA and part of the corresponding genomic clone. The cDNA clones were isolated from a library made with mRNA from induced human fibroblasts. As, however, the information thus obtained was still incomplete, genomic clones were isolated from a total human DNA library. In this way, the entire region coding for the 26-kDa protein was established, as well as the neighbouring sequences including the inducible promoter area. From the deduced polypeptide sequence a number of characteristics of the 26-kDa protein can be explained. It turns out that the 26-kDa protein gene and the so-called 'IFN-beta 2' gene are identical. However, extensive homology searches indicate that the 26-kDa protein does not show statistically significant sequence homology with any known interferon species. Hence, the question of whether the 26-kDa product represents a novel IFN species remains open.
Fiber-Optic DNA Sensor for Fluorometric Nucleic Acid Determination (1995)

Single-stranded deoxyribonucleic acid (ssDNA) thymidylic acid icosanucleotides (dT20) were synthesized on the surfaces of derivatized quartz optical fibers to create an optical DNA biosensor. The synthesis made use of an automated solid-phase synthesizer and phosphoramidite synthons. The covalently immobilized oligomers were found to hybridize with complementary ssDNA (cDNA) or ssRNA (cRNA) from solution, and the device was regenerable for multiple cycles of application. Hybridization on optical fibers was detected by the use of the fluorescent DNA stain ethidium bromide (EB). The procedure used hybridization assay techniques and provided a detection limit of 86 ng x mL(-1) cDNA and a sensitivity of 200% fluorescence intensity increase per 100 ng x mL(-1) of cDNA, with one cycle of hybridization analysis requiring 45 min. The sensor has been observed to be regenerable (minimum of five cycles) and to sustain full activity after prolonged storage times (1 year), harsh washing conditions (sonication), and sterilization (autoclaving). The extent of hybridization between the immobilized and complementary nucleic acid strands was determined by UV absorbance thermal denaturation studies wherein all 20 bases on each strand of the nucleic acid were found to be involved in duplex formation.
Raman scattering and photoluminescence from Si nanoparticles in annealed SiOx thin films (2002)

Silicon-rich silicon oxide thin films have been prepared by thermal evaporation of silicon monoxide in vacuum. The SiOx film composition (1.1⩽ x ⩽1.7) has been controlled by varying the deposition rate and residual pressure in the chamber. Long time stability of all films has been ensured by a postdeposition annealing at 523 K for 30 min in Ar atmosphere. Some films were further annealed at 973 K and some others at 1303 K. Raman scattering measurements have implied the formation of amorphous silicon nanoparticles in films annealed at 973 K and Si nanocrystals in films annealed at 1303 K. The latter conclusion is strongly supported by high resolution electron microscopy studies which show a high density of Si nanocrystals in these films. Photoluminescence has been observed from both amorphous and crystalline nanoparticles and interpreted in terms of band-to-band recombination in the nanoparticles having average size greater than 2.5 nm and carrier recombination through defect states in smaller nanoparticles.
Sterilization of Thiol-ene/Acrylate Based Shape Memory Polymers for Biomedical Applications (2016)

A fundamental study on the sterilization of thiol-ene/acrylate polymers for biomedical applications is presented. These polymer networks belong to the emerging field of shape memory polymers and have the capability to undergo softening after insertion into the body. The impact of various sterilization methods, such as radiation, steam, and ethylene oxide on the thermomechanical properties of these stimuli responsive materials is investigated. Time and temperature dependent thermomechanical properties of sterilized and nonsterilized samples are determined by means of dynamic mechanical analysis in an aqueous environment to allow testing of polymers in phosphate buffered saline. The findings show that ethylene oxide sterilization is appropriate for thiol-ene and thiol-ene/acrylate based shape memory polymers. This method does not adversely affect thermomechanical and self-softening properties and after sterilization, endotoxin levels remain below the thresholds recommended in the FDA Guidance.
Pyrrolysine is not hardwired for cotranslational insertion at UAG codons (2007)

Pyrrolysine (Pyl), the 22nd naturally encoded amino acid, gets acylated to its distinctive UAG suppressor tRNA(Pyl) by the cognate pyrrolysyl-tRNA synthetase (PylRS). Here we determine the RNA elements required for recognition and aminoacylation of tRNA(Pyl) in vivo by using the Pyl analog N-epsilon-cyclopentyloxycarbonyl-l-lysine. Forty-two Methanosarcina barkeri tRNA(Pyl) variants were tested in Escherichia coli for suppression of the lac amber A24 mutation; then relevant tRNA(Pyl) mutants were selected to determine in vivo binding to M. barkeri PylRS in a yeast three-hybrid system and to measure in vitro tRNA(Pyl) aminoacylation. tRNA(Pyl) identity elements include the discriminator base, the first base pair of the acceptor stem, the T-stem base pair G51:C63, and the anticodon flanking nucleotides U33 and A37. Transplantation of the tRNA(Pyl) identity elements into the mitochondrial bovine tRNA(Ser) scaffold yielded chimeric tRNAs active both in vitro and in vivo. Because the anticodon is not important for PylRS recognition, a tRNA(Pyl) variant could be constructed that efficiently suppressed the lac opal U4 mutation in E. coli. These data suggest that tRNA(Pyl) variants may decode numerous codons and that tRNA(Pyl):PylRS is a fine orthogonal tRNA:synthetase pair that facilitated the late addition of Pyl to the genetic code.
A serum-resistant cytofectin for cellular delivery of antisense oligodeoxynucleotides and plasmid DNA. (1996)

Development of antisense technology has focused in part on creating improved methods for delivering oligodeoxynucleotides (ODNs) to cells. In this report, we describe a cationic lipid that, when formulated with the fusogenic lipid dioleoylphosphatidyliethanolamine, greatly improves the cellular uptake properties of antisense ODNs, as well as plasmid DNA. This lipid formulation, termed GS 2888 cytofectin, (i) efficiently transfects ODNs and plasmids into many cell types in the presence or absence of 10% serum in the medium, (ii) uses a 4- to 10-fold lower concentration of the agent as compared to the commercially available Lipofectin liposome, and (iii) is > or = 20-fold more effective at eliciting antisense effects in the presence of serum when compared to Lipofectin. Here we show antisense effects using GS 2888 cytofectin together with C-5 propynyl pyrimidine phosphorothioate ODNs in which we achieve inhibition of gene expression using low nanomolar concentrations of ODN. This agent expands the utility of antisense ODNs for their use in understanding gene function and offers the potential for its use in DNA delivery applications in vivo.
[Retracted] Cooperation Mode of Soccer Robot Game Based on Improved SARSA Algorithm (2022)

Football is one of the most popular sports in the world, and its competition has received increasing people’s attention. With the increasing number of robot football competitions, more strategic planning is needed for robot football matches. In a tournament, each player has their own task and must have the skills to complete it. In this paper, we use the RoboCup2D platform to give details of the server and client roles, introduce the agent model in RoboCup2D, and compare the plan design and scheme design presented in the current study using the SARSA algorithm, one of the augmented methods classified as TD learning metrics. In addition, heuristic information was introduced and implemented to enhance learning through the sharing of Q values between participants and reinforcement learning. A comparative analysis of the feasibility of the SARSA algorithm in the context of its application in RoboCup2D was carried out, and the experimental results proved that our algorithm was effective in improving the team’s offensive and defensive capabilities.
Identification of a Chemical Probe for Bromo and Extra C-Terminal Bromodomain Inhibition through Optimization of a Fragment-Derived Hit (2012)

The posttranslational modification of chromatin through acetylation at selected histone lysine residues is governed by histone acetyltransferases (HATs) and histone deacetylases (HDACs). The significance of this subset of the epigenetic code is interrogated and interpreted by an acetyllysine-specific protein-protein interaction with bromodomain reader modules. Selective inhibition of the bromo and extra C-terminal domain (BET) family of bromodomains with a small molecule is feasible, and this may represent an opportunity for disease intervention through the recently disclosed antiproliferative and anti-inflammatory properties of such inhibitors. Herein, we describe the discovery and structure-activity relationship (SAR) of a novel, small-molecule chemical probe for BET family inhibition that was identified through the application of structure-based fragment assessment and optimization techniques. This has yielded a potent, selective compound with cell-based activity (PFI-1) that may further add to the understanding of BET family function within the bromodomains.
HV dielectric strength of shielding electrodes in vacuum circuit-breakers (2003)

The subject of this paper is the HV dielectric strength of shielding and field-grading electrodes in vacuum circuit-breakers. It describes the effects exerted by various geometrical parameters, the surface area and the material of the shielding on the breakdown behavior of shielding. The experiments were performed with a shielding electrodes model and were carried out under lightning impulse voltage. The dielectric results are discussed by means of field simulations.
Improved Metabolic Control and Quality of Life in Seven Patients With Type 1 Diabetes Following Islet After Kidney Transplantation (2008)

Islet transplantation represents a feasible therapeutic option for patients with T1DM bearing a stable kidney allograft. Insulin independence at 1 year is lower than what reported in islet transplant alone. Nevertheless, clear benefits in terms of optimal metabolic control and absence of severe hypoglycemia are invariably present.
Loss of the multifunctional RNA-binding protein RBM47 as a source of selectable metastatic traits in breast cancer (2014)

The mechanisms through which cancer cells lock in altered transcriptional programs in support of metastasis remain largely unknown. Through integrative analysis of clinical breast cancer gene expression datasets, cell line models of breast cancer progression, and mutation data from cancer genome resequencing studies, we identified RNA binding motif protein 47 (RBM47) as a suppressor of breast cancer progression and metastasis. RBM47 inhibited breast cancer re-initiation and growth in experimental models. Transcriptome-wide HITS-CLIP analysis revealed widespread RBM47 binding to mRNAs, most prominently in introns and 3'UTRs. RBM47 altered splicing and abundance of a subset of its target mRNAs. Some of the mRNAs stabilized by RBM47, as exemplified by dickkopf WNT signaling pathway inhibitor 1, inhibit tumor progression downstream of RBM47. Our work identifies RBM47 as an RNA-binding protein that can suppress breast cancer progression and demonstrates how the inactivation of a broadly targeted RNA chaperone enables selection of a pro-metastatic state.
Hemorrhage Control in the Battlefield: Role of New Hemostatic Agents (2005)

Uncontrolled hemorrhage is the leading cause of preventable combat-related deaths. The vast majority of these deaths occur in the field before the injured can be transported to a treatment facility. Early control of hemorrhage remains the most effective strategy for treating combat casualties. A number of hemostatic agents have recently been deployed to the warfront that can be used to arrest bleeding before surgical control of the source. The purpose of this article is to summarize the background information regarding these hemostatic agents, indications and rationale for their use, and characteristics of these products that may impact effectiveness.
Expression of CCL5 (RANTES) and CCR5 in prostate cancer (2005)

In light of evidence that inflammation influences the pathogenesis of PCa, these results suggest that inflammatory chemokines, such as CCL5, expressed by prostate cells may act directly on the growth and survival of PCa cells. Chemokine receptor antagonists may thus block autocrine mechanisms of PCa progression.
NMR determination of EPR hyperfine coupling constants of some 5-(n-alkyl)-1,1,3,3-tetrakis(trideuteriomethyl)isoindolin-2-yloxyls (1994)

2D 1H–13C chemical shift correlation spectroscopy has been used to determine some small 1H and 13C EPR hyperfine interaction constants in some 5-(n-alkyl)isoindolin-2-yloxyl radicals. In the methodology used, it was necessary to measure the chemical shifts of both the isoindolines and of the cognate isoindolin-2-yloxyl radicals. The latter n-alkyl-substituted radicals have considerable potential as EPR spin probes in lipid systems.
LUMINESCENCE DATING WORK FROM THE HEIDELBERG GROUP: A KEY TECHNOLOGY IN GEOARCHAEOLOGY (2010)

Geoarchaeology is a growing discipline in archaeological science. It aims at the natural environ‐ ment as context of past human societies and at the interaction between both, the environment and man as part of a joint ecosystem. This topic is also of considerable concern of present societies. Like other historic sciences, geoarchaeology requires accurate chronologies. Since one deals in geoar‐ chaeology predominantly with sediments and rocks, luminescence methods play a key role. This is demonstrated in two case studies from Phlious in southern Greece and Nasca in southern Peru. The results show clearly climatically triggered social developments and feedbacks to the environment.
P2P-Paid: A Peer-to-Peer Wireless Payment System (2005)

The fast advance of wireless networking, communication, and mobile technology is making a big impact to daily life. The significant increase of mobile device users in the recent years causes a strong demand on secured wireless information services and reliable mobile commerce applications. Since wireless payment is a critical part of most wireless information services and mobile commerce applications, how to generate secured mobile payment systems becomes a hot research topic in both the e-commerce research community and wireless commerce industry. This paper proposes a peer-to-peer wireless payment system, known as P2P-Paid, to allow two mobile users to conduct wireless payment transactions over the Bluetooth communications. The system uses a 2-dimensional secured protocol, which not only supports the peer-to-peer (P2P) payment transactions between two mobile clients using Bluetooth communications, but also supports the related secured transactions between the payment server and mobile clients. This paper provides a system overview about system functional features, system architecture, and used technologies. Moreover, an integrated security solution for the P2P-Paid system is described. Our first phase implementation is reported, and application examples are given to demonstrate the functions and feasibility of this system.
Bronchodilator actions of xanthine derivatives administered by inhalation in asthma. (1985)

The airway response to the inhalation of four alkyl xanthines was studied in 17 subjects with moderately severe asthma (mean FEV1 1.19 litres, 42% predicted). Theophylline (10 mg/ml), glycine theophyllinate (50 mg/ml), theophylline ethylenediamine (aminophylline 50 mg/ml), and diprophylline (125 mg/ml) were administered by nebulisation and the airway response was measured as percentage change from baseline of specific airway conductance (sGaw). All xanthine derivatives had an unpleasant taste and produced coughing at the onset of nebulisation. All four xanthines produced a significant increase in sGaw by comparison with saline placebo, with a maximum mean increase from baseline of 35% for theophylline, 40% for glycine theophyllinate, 60% for aminophylline, and 32% for diprophylline. Inhalation of 200 micrograms salbutamol from a metered dose inhaler produced an additional increase in sGaw of 149%. Thus alkyl substituted xanthines administered by inhalation to patients with asthma cause significant short lived bronchodilatation, but this effect is small compared with that of a conventional dose of an inhaled beta 2 adrenoceptor agonist.
Fluorescent Quantum Dots as Artificial Antennas for Enhanced Light Harvesting and Energy Transfer to Photosynthetic Reaction Centers (2010)

Bio–nano: Quantum dots (QDs) can be tagged with photosynthetic reaction centers (RCs) in such a way (see picture) that FRET from the QD to the RC leads to a near-threefold increase in the rate of generation of excitons in the RC over that in the RC alone. Even-stronger enhancements are predicted, thus indicating that such complexes may significantly improve the efficiency of photosynthesis. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Determination of dissolved oxygen based on photoinduced electron transfer from quantum dots to methyl viologen (2010)

A rapid colorimetric methodology based on photoinduced electron transfer from excited CdS quantum dots (CdS QDs) to methyl viologen (MV2+) has been developed for the sensitive determination of dissolved oxygen (DO) content. The reduction of MV2+ is initiated by light excitation of CdS QDs, which induces the electron transfer from sacrificial donor glutathione (GSH) to the photogenerated hole of CdS QDs. Due to the presence of oxygen, the reduced radical-cation of methyl viologen (MV+˙) could be rapidly reoxidized, thus turning into the original methyl viologen dication (MV2+). The existence of MV+˙ is confirmed by electron paramagnetic resonance technique. This proposed colorimetric methodology has been applied to quantify the concentration of DO in real water samples. The result is consistent with the value obtained while adopting the traditional standard Winkler's methodology. A linear response of the absorbance of CdS QDs-GSH-MV system to the different concentrations of DO is obtained in the range of 0.75∼7.95 ppm, with a detection limit of 0.23 ppm in pH 7.5.
Efficient partial enumeration for timing analysis of asynchronous systems (1996)

Article Efficient partial enumeration for timing analysis of asynchronous systems Share on Authors: Eric Verlind IMEC, Kapeldreef 75, B-3001 Leuven, Belgium IMEC, Kapeldreef 75, B-3001 Leuven, BelgiumView Profile , Gjalt de Jong IMEC, Kapeldreef 75, B-3001 Leuven, Belgium IMEC, Kapeldreef 75, B-3001 Leuven, BelgiumView Profile , Bill Lin IMEC, Kapeldreef 75, B-3001 Leuven, Belgium IMEC, Kapeldreef 75, B-3001 Leuven, BelgiumView Profile Authors Info & Claims DAC '96: Proceedings of the 33rd annual Design Automation ConferenceJune 1996 Pages 55–58https://doi.org/10.1145/240518.240529Online:01 June 1996Publication History 8citation167DownloadsMetricsTotal Citations8Total Downloads167Last 12 Months1Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Silicon Nanowire Arrays for Label-Free Detection of DNA (2007)

Arrays of highly ordered n-type silicon nanowires (SiNW) are fabricated using complementary metal-oxide semiconductor (CMOS) compatible technology, and their applications in biosensors are investigated. Peptide nucleic acid (PNA) capture probe-functionalized SiNW arrays show a concentration-dependent resistance change upon hybridization to complementary target DNA that is linear over a large dynamic range with a detection limit of 10 fM. As with other SiNW biosensing devices, the sensing mechanism can be understood in terms of the change in charge density at the SiNW surface after hybridization, the so-called "field effect". The SiNW array biosensor discriminates satisfactorily against mismatched target DNA. It is also able to monitor directly the DNA hybridization event in situ and in real time. The SiNW array biosensor described here is ultrasensitive, non-radioactive, and more importantly, label-free, and is of particular importance to the development of gene expression profiling tools and point-of-care applications.
Hospital outcomes associated with guideline‐recommended antibiotic therapy for pediatric pneumonia (2014)

Use of guideline-recommended antibiotic therapy was not associated with unintended negative consequences; there were no changes in LOS, total costs, or inpatient pharmacy costs.
Association of human class I MHC alleles with the adenovirus E3/19K protein. (1994)

A panel of HLA-A and -B locus products was analyzed for their ability to associate with the adenovirus E3/19K (E19) protein in a co-immunoprecipitation assay. Three general categories of binding were identified. HLA-A2.1 and -B7 bind very well to E19. Compared with A2.1, 6- to 30-fold less E19 was associated with HLA-A3, -A1, and -Aw69; 50- to 150-fold less E19 was associated with HLA-Aw68, -B27, and -Bw58. Digestion with endoglycosidase H indicated that all levels of association resulted in inhibition of intracellular transport and processing, however, a fraction of Aw68, B27, and Bw58 escaped from intracellular retention. In contrast to the human class I molecules analyzed, transport of the murine H-2Dd molecule was not inhibited in the presence of E19. Hybrid class I molecules, in which exons encoding domains of A2.1 and H-2Dd had been exchanged, were used to define the regions of A2.1 required for E19 association. The alpha 1 and alpha 2 domains of A2.1 contain the minimum residues necessary for both stable association with E19 and subsequent inhibition of transport. A hybrid construct containing only the alpha 2 domain of A2.1 associated weakly with E19, but its post-translational processing was completely inhibited. In contrast, although a construct containing only the alpha 1 domain of A2.1 also associated weakly with E19, its intracellular transport was slowed rather than completely inhibited. Taken together, these results indicate that residues in both the alpha 1 and alpha 2 domains of A2.1 and Dd can influence stable binding of E19, with the phenotypic changes dominated by the origin of the alpha 2 domain.