gebhart/patrepeval huggingface dataset:
Reduced-Time Facsimile Transmission by Digital Coding (1961)

The growing importance of digital communication links has resulted in a family of coding methods designed to achieve reduced-time digital facsimile transmission. This paper reports on a study of the effectiveness and feasibility of one of these methods, Run Length Coding (RLC). RLC is a well-known digital method in which the area and shade of the copy are quantized to form a mosaic pattern. Neighboring elements of the mosaic are successively scanned, and redundancy is removed by coding and transmitting only lengths between shade changes (run lengths) rather than individual area elements. Measurements of the statistical distribution of run lengths of various copy were made to determine the possible transmission time reduction. Digital equipment and a drum scanner were used to measure the statistics of weather maps, topographic maps, text, diagrams, and continuous-tone pictures (quantized to four shade levels). The maximum possible time reduction was calculated for each type of copy, and practical codes were then designed and evaluated. The average time reduction factor for black and white copy was four, compared to a theoretical optimum of 4.7. Since the maximum bit rate of the transmission line should be continuously utilized, and since time reduction factors for particular runs depend on the lengths of the runs, a practical RLC facsimile system must possess either a variable speed scan, a large and elastic buffer storage, or some combination of the two. A system is proposed whose encoding principle permits a feasible compromise between these two extremes, and whose implementation involves a flying-spot scanner that is converted into a digital device by means of optical reference gratings.
Video adaptation proxy for wireless Dynamic Adaptive Streaming over HTTP (2012)

This research studies Dynamic Adaptive Streaming over HTTP (DASH) in cellular broadband access networks (e.g. HSDPA, HSPA+, LTE). We propose a wireless DASH (WiDASH) proxy to enhance the Quality-of-Experience (QoE) of wireless DASH. WiDASH proxy locates at the edge between Internet and wireless core networks. Different from conventional DASH, whose rate adaptation logics are either implemented locally in the user equipment or in the DASH server, WiDASH proxy is in charge of video adaptation, which makes it feasible to perform global optimization over multiple concurrent DASH flows. WiDASH proxy improves DASH QoE by three novel technologies. The first one is architecture level innovation. WiDASH simultaneously explores the potential of both split TCP and parallel TCP. It splits the original TCP connection from DASH server to a wireless user into one wired TCP and multiple wireless TCPs. This approach is able to increase video streams' average throughput without sacrificing inter user fairness. Second, WiDASH proxy employs video quality aware dynamic prioritization. Low bit rate video streams are entitled with high priority to guarantee minimum QoE for users with bad wireless channel quality. A video stream's priority is gradually dropped to normal state with the growth of its bit rate so that inter flow fairness between video streams and background best effort flows are still maintained. Third, we design a multiple-input multiple-output adaptive optimal controller, based on adaptive control theory. This rate controller minimizes a quadratic cost function which is defined as the weighted sum of multiple concurrent DASH videos' distortion, bit rate variation, and playback jitter. The solution of the optimization problem implicitly coordinates multiple DASH streams to improve overall streaming QoE. Simulation results verify that WiDASH proxy is able to improve QoE by providing smoother video streams with higher average visual quality for wireless HTTP video streaming.
Depressive behavior and activation of the orexin/hypocretin system. (2013)

The orexin/hypocretin peptide signaling system plays a neuromodulatory role in motivation and stress; two critical components of depression. Although work has been done to identify links between orexin and depression, few specific neuroanatomical associations have been made. These studies have not investigated the relationship between orexin and orexin receptor expression in specific brain regions associated with this disorder. To address this, we examined immobility during the forced swim test (FST) in mice, a commonly used measure of depressive behavior. We analyzed the variation in FST immobility with the distribution of orexin and its receptor mRNA. We found that animals that exhibited more robust depressive behavior had greater or lesser orexin system expression that depended on the limbic brain region analyzed. In the hippocampus there was a negative correlation between orexin expression and FST immobility. Animals that displayed relatively more depressive behavior had lower hippocampal expression of Orexin A (OrxA). In the amygdala, there was a curvilinear relationship between OrxA and FST performance. In addition there was a positive correlation with amygdalar Type I orexin receptor (Orx1) mRNA and depressive behavior. Despite the differences in limbic orexin expression, there was no correlation between immobility and hypothalamic orexin neuron activation as measured by c-Fos. Overall, more severe depressive behavior was associated with reduced hippocampal orexin expression, contrasted with increased orexin plus Orx1 receptor mRNA expression in the amygdala. This divergent pattern between the hippocampus and amygdala mirrors a neurobiological theme seen in depression resulting from reduced hippocampal, but increased amygdalar, size and function.
The Role of Allergy in the Severity of Nasal Polyposis (2007)

The results of this study show that, although patients with nasal polyposis frequently have allergies, the presence of allergy does not correlate with polyp size, symptom scores or rate of recurrence.
Altering central nervous system physiology with a defective herpes simplex virus vector expressing the glucose transporter gene. (1993)

Because of their postmitotic nature, neurons are difficult subjects for gene transfer. To circumvent this, we have used a defective herpes simplex virus vector to overexpress the rat brain glucose transporter (GT) gene under the control of the human cytomegalovirus ie1 promoter. This vector, designated vIE1GT, was propagated using a herpes simplex virus type 1 temperature-sensitive mutant, ts756. GT expressed from vIE1GT was readily immunoprecipitated from membrane fractions of vIE1GT-infected Vero cells. By using indirect double immunofluorescence techniques, vIE1GT was shown to be capable of enhancing GT expression in cultured hippocampal neurons and glia. Glucose transport in such vIE1GT-infected cultures was increased approximately 2-fold relative to controls. The efficacy of this system in vivo was then tested by microinjection of vIE1GT into adult rat hippocampus. When examined 2 days later, GT expression from vIE1GT was demonstrated in hippocampal neurons by in situ hybridization; a small but significant increase in glucose transport was detected in tissue immediately surrounding the injection site by 2-deoxy[14C]glucose uptake and autoradiography. Such injections did not cause marked cytopathology. Thus, this approach can be used to alter central nervous system physiology in vitro and in vivo.
Problems in automatic abstracting (1964)

FIG. The program can be easily adjusted to solve a large variety of menu problems witl~ different sets of objectives.
A Compendium of Signals and Responses Triggered by Prodeath and Prosurvival Cytokines (2005)

Cell-signaling networks consist of proteins with a variety of functions (receptors, adaptor proteins, GTPases, kinases, proteases, and transcription factors) working together to control cell fate. Although much is known about the identities and biochemical activities of these signaling proteins, the ways in which they are combined into networks to process and transduce signals are poorly understood. Network-level understanding of signaling requires data on a wide variety of biochemical processes such as posttranslational modification, assembly of macromolecular complexes, enzymatic activity, and localization. No single method can gather such heterogeneous data in high throughput, and most studies of signal transduction therefore rely on series of small, discrete experiments. Inspired by the power of systematic datasets in genomics, we set out to build a systematic signaling dataset that would enable the construction of predictive models of cell-signaling networks. Here we describe the compilation and fusion of approximately 10,000 signal and response measurements acquired from HT-29 cells treated with tumor necrosis factor-alpha, a proapoptotic cytokine, in combination with epidermal growth factor or insulin, two prosurvival growth factors. Nineteen protein signals were measured over a 24-h period using kinase activity assays, quantitative immunoblotting, and antibody microarrays. Four different measurements of apoptotic response were also collected by flow cytometry for each time course. Partial least squares regression models that relate signaling data to apoptotic response data reveal which aspects of compendium construction and analysis were important for the reproducibility, internal consistency, and accuracy of the fused set of signaling measurements. We conclude that it is possible to build self-consistent compendia of cell-signaling data that can be mined computationally to yield important insights into the control of mammalian cell responses.
A Thermodynamic Investigation into the Binding Properties of DNA Functionalized Gold Nanoparticle Probes and Molecular Fluorophore Probes (2005)

We report the first quantitative analysis of the oligonucleotide binding thermodynamics for DNA functionalized gold nanoparticle probes and compare our findings to molecular fluorophore probes on a sequence-for-sequence basis. With proper design, nanoparticle probes show significantly increased binding over molecular fluorophore probes under identical conditions. This is significant because probe binding strength directly influences detection sensitivity limits.
Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes (2014)

Modified mRNA cap analogs aid in the study of mRNA-related processes and may enable creation of novel therapeutic interventions. We report the synthesis and properties of 11 dinucleotide cap analogs bearing a single boranophosphate modification at either the α-, β- or γ-position of the 5',5'-triphosphate chain. The compounds can potentially serve either as inhibitors of translation in cancer cells or reagents for increasing expression of therapeutic proteins in vivo from exogenous mRNAs. The BH3-analogs were tested as substrates and binding partners for two major cytoplasmic cap-binding proteins, DcpS, a decapping pyrophosphatase, and eIF4E, a translation initiation factor. The susceptibility to DcpS was different between BH3-analogs and the corresponding analogs containing S instead of BH3 (S-analogs). Depending on its placement, the boranophosphate group weakened the interaction with DcpS but stabilized the interaction with eIF4E. The first of the properties makes the BH3-analogs more stable and the second, more potent as inhibitors of protein biosynthesis. Protein expression in dendritic cells was 2.2- and 1.7-fold higher for mRNAs capped with m2 (7,2'-O)GppBH3pG D1 and m2 (7,2'-O)GppBH3pG D2, respectively, than for in vitro transcribed mRNA capped with m2 (7,3'-O)GpppG. Higher expression of cancer antigens would make mRNAs containing m2 (7,2'-O)GppBH3pG D1 and m2 (7,2'-O)GppBH3pG D2 favorable for anticancer immunization.
Molecular evolution and epidemiology of dengue-3 viruses (1994)

The nucleic acid sequences of the pre-membrane/membrane and envelope protein genes of 23 geographically and temporally distinct dengue (DEN)-3 viruses were determined. This was accomplished by reverse transcriptase-PCR amplification of the structural genes followed by automated DNA sequence analysis. Comparison of nucleic acid sequences revealed that similarity among the viruses was greater than 90%. The similarity among deduced amino acids was between 95% and 100%, and in many cases identical amino acid substitutions occurred among viruses from similar geographical regions. Alignment of nucleic acid sequences followed by parsimony analysis allowed the generation of phylogenetic trees, demonstrating that geographically independent evolution of DEN-3 viruses had occurred. The DEN-3 viruses were separated into four genetically distinct subtypes. Subtype I consists of viruses from Indonesia, Malaysia, the Philippines and the South Pacific islands; subtype II consists of viruses from Thailand; subtype III consists of viruses from Sri Lanka, India, Africa and Samoa; subtype IV consists of viruses from Puerto Rico and the 1965 Tahiti virus. Phylogenetic analysis has also contributed to our understanding of the molecular epidemiology and worldwide distribution of DEN-3 viruses.
Ablation of Osteopontin Improves the Skeletal Phenotype of Phospho1 −/− Mice (2014)

PHOSPHO1 and tissue-nonspecific alkaline phosphatase (TNAP) have nonredundant functions during skeletal mineralization. Although TNAP deficiency (Alpl(-/-) mice) leads to hypophosphatasia, caused by accumulation of the mineralization inhibitor inorganic pyrophosphate (PPi ), comparably elevated levels of PPi in Phospho1(-/-) mice do not explain their stunted growth, spontaneous fractures, bowed long bones, osteomalacia, and scoliosis. We have previously shown that elevated PPi in Alpl(-/-) mice is accompanied by elevated osteopontin (OPN), another potent mineralization inhibitor, and that the amount of OPN correlates with the severity of hypophosphatasia in mice. Here we demonstrate that plasma OPN is elevated and OPN expression is upregulated in the skeleton, particularly in the vertebrae, of Phospho1(-/-) mice. Liquid chromatography/tandem mass spectrometry showed an increased proportion of phosphorylated OPN (p-OPN) peptides in Phospho1(-/-) mice, suggesting that accumulation of p-OPN causes the skeletal abnormalities in Phospho1(-/-) mice. We also show that ablation of the OPN gene, Spp1, leads to improvements in the skeletal phenotype in Phospho1(-/-) as they age. In particular, their scoliosis is ameliorated at 1 month of age and is completely rescued at 3 months of age. There is also improvement in the long bone defects characteristic of Phospho1(-/-) mice at 3 months of age. Mineralization assays comparing [Phospho1(-/-) ; Spp1(-/-) ], Phospho1(-/-) , and Spp1(-/-) chondrocytes display corrected mineralization by the double knockout cells. Expression of chondrocyte differentiation markers was also normalized in the [Phospho1(-/-) ; Spp1(-/-) ] mice. Thus, although Alpl and Phospho1 deficiencies lead to similar skeletal phenotypes and comparable changes in the expression levels of PPi and OPN, there is a clear dissociation in the hierarchical roles of these potent inhibitors of mineralization, with elevated PPi and elevated p-OPN levels causing the respective skeletal phenotypes in Alpl(-/-) and Phospho1(-/-) mice.
System for automated validation of embedded software in multiple operating configurations (2003)

Embedded controllers in safety critical applications rely on the highest of software quality standards. Testing is performed to ensure that requirements and specifications are met for all the different environments in which the controllers operate. This paper describes the architecture of a system that uses a relational database for tracking tests, requirements, and configurations. The relational sub-schemas are integrated in a data warehouse and allow traceability from requirements to tests. A normalized representation of test cases enables the system to reason about the test topologies and is used for constructing clusters of similar tests. A representative test from each cluster can in turn provide a rapid estimation, of the software's requirement coverage and quality.
Predictors of Response to Cyclo-Oxygenase-2 Inhibitors in Osteoarthritis: Pooled Results from Two Identical Trials Comparing Etoricoxib, Celecoxib, and Placebo (2011)

Lower levels of physical function decreased the odds of a response to NSAID treatment in OA, although the clinical significance is unknown given the small differences between responders and nonresponders. No other measured baseline variables consistently predicted response in these studies, which may reflect the known individual variability in NSAID response.
Monitoring of Bone Marrow Cell Homing Into the Infarcted Human Myocardium (2005)

18F-FDG labeling and 3D PET imaging can be used to monitor myocardial homing and biodistribution of BMCs after therapeutic application in patients.
Cationic amphipathic peptides, derived from bovine and human lactoferrins, with antimicrobial activity against oral pathogens (1999)

Peptides derived from the N-terminal domain that comprises an amphipathic α-helix in human lactoferrin (LFh 18–31 and LFh 20–38) and bovine lactoferrin (LFb 17–30 and LFb 19–37) were chemically synthesised. Since many positively charged amphipathic α-helices contain antimicrobial activity, the peptides were tested for their antimicrobial activity against various oral pathogens. Both peptides from bovine lactoferrin had more potent antimicrobial activities than the human equivalents. Peptide LFb 17–30, containing the largest number of positively charged amino acids, showed the highest antimicrobial activity to both Gram-positive and Gram-negative bacteria. Since native lactoferrin molecules had no killing activity, release of these peptides from the native protein should be investigated to explore the use in oral care products.
Recombinant anti‐sialidase single‐chain variable fragment antibody (1994)

The single-chain antibody variable fragment (scFv), with a 15-residue polypeptide linker (Gly4Ser)3, of monoclonal antibody NC10 was expressed in Escherichia coli and purified to homogeneity. This scFv molecule, refolded from 6 M guanidine hydrochloride, was predominantly a monomer of 27 kDa and was stable on storage at 4 degrees and 20 degrees C. At higher protein concentrations (approximately 5 mg/ml) dimer and higher-molecular-mass multimers were formed and freezing enhanced this aggregation. The dimer was not stable and dissociated to monomer at 20 degrees C with a half-life of approximately 8 days. The higher-molecular-mass multimers and dimer dissociated to monomer in 60% ethylene glycol. Both the monomer and dimer were active and with tern N9 sialidase yielded complexes of 276 kDa and 569 kDa, respectively, indicating that four scFv molecules bound/sialidase tetramer and that the dimer was bivalent and cross-linked two sialidase tetramers. Binding studies at low concentrations and using radiolabelled scFv indicated that the binding affinity of the dimer was approximately twofold higher than that of the monomer, and the binding affinities of the scFv were similar to that of the parent NC10 antigen-binding fragment (Fab) molecule. A complex between tern N9 sialidase and NC10 scFv was crystallized and the structure of the complex was solved at 0.3-nm resolution by X-ray diffraction. Comparison of this scFv/sialidase structure with the parent Fab/sialidase structure revealed that the modes of attachment of scFv and Fab to sialidase were very similar. There was no discernible electron density for the peptide linker joining the variable heavy (VH) and variable light (VL) chains. A close interaction between two symmetry-related scFv suggests that they may have crystallized as dimers.
Altering the Expression of the Chlorophyllase GeneATHCOR1 in Transgenic Arabidopsis Caused Changes in the Chlorophyll-to-Chlorophyllide Ratio (2002)

The Arabidopsis gene ATHCOR1, which encodes the CORI1 (coronatine-induced) protein, was expressed in bacterial cells. Soluble recombinant CORI1 was purified and shown to possess chlorophyllase (Chlase) activity in vitro. To determine its activity in vivo, wild-type Arabidopsis and coi1 mutant, which lacks ATHCOR1 transcripts, were transformed with sense and antisense forms of the gene. Wild-type and coi1 plants overexpressing ATHCOR1 showed increased contents of chlorophyllide (Chlide) without a substantial change in the total amount of the extractable chlorophyll (Chl). These plants presented high Chlide to Chl ratios in leaves, whereas antisense plants and nontransformed coi1 mutant showed undetectable ATHCOR1 mRNA and significantly lower Chlide to Chl ratios, relative to wild-type control. Overexpression of ATHCOR1 caused an increased breakdown of Chl a, as revealed by the Chlide a to b ratio, which was significantly higher in sense than wild-type, coi1 mutant, and antisense plants. This preferential activity of CORI1 toward Chl a was further supported by in vitro analyses using the purified protein. Increased Chlase activity was detected in developing flowers, which correlated to the constitutive expression of ATHCOR1 in this organ. Flowers of the antisense plant showed reduced Chlide to Chl ratio, suggesting a role of CORI1 in Chl breakdown during flower senescence. The results show that ATHCOR1 has Chlase activity in vivo, however, because coi1 flowers have no detectable ATHCOR1 mRNA and present Chlide to Chl ratios comparable with the wild type, an additional Chlase is likely to be active in Arabidopsis. In accordance, transcripts of a second Arabidopsis Chlase gene, AtCLH2, were detected in both normal and mutant flowers.
A review of the various approaches to a silicon laser (2003)

Silicon is the leading material concerning high-density electronic functionality. Integration and economy of scale are the two keys ingredients for the silicon technological success. The present interconnection degree is sufficient to cause interconnect propagation delays, overheating and information latency between single devices. The overcome of this <i>Interconnection bottleneck </i>is together the main motivation and opportunity for the present-day silicon microphotonics, where attempts to combine photonic and electronic components on a single Si chip or wafer are strongly pursued. The main limitation of this technology when is implemented in silicon is the lack of any practical silicon light sources: either an efficient LEDs or a Si lasers. Despite of all, during these last 10 years many different strategies have been employed to overcome these materials limitations and silicon light emitting diodes (LED) are now only a factor of ten out of the severe market requirements. The main future challenge for silicon microphotonics is the demonstration of a silicon-based laser action to engineer a silicon laser. During the nineties many research efforts have been focused to this goal. A steady improvement in silicon LED performances has been achieved. However it was only at the end of 2000 and during 2001 that many breakthroughs have been demonstrated showing that this field is very active and still promising. The up to now principal approaches can be subdivided into the following categories: 1. Bulk silicon with extremely high non-radiative lifetime 2. Silicon nanocrystals 3. Doping of silicon with rare earths ions 4. Direct band-gap group IV alloys and quantum confined Si or Ge or group IV alloy structures 5. Quantum cascade Si/Ge structures During the presentation a critical review of all these approaches will be performed and the most suitable candidate will be underlined.