gebhart/patrepeval huggingface dataset:
THE THREE-DIMENSIONAL STRUCTURE OF THE RIBOSOME AND ITS COMPONENTS (1998)

Exciting progress has been made in the last decade by those who use physical methods to study the structure of the ribosome and its components. The structures of 10 ribosomal proteins and three isolated ribosomal protein domains are known, and the conformations of a significant number of rRNA sequences have been determined. Electron microscopists have made major advances in the analysis of images of ribosomes, and microscopically derived ribosome models at resolutions approaching 10A are likely quite soon. Furthermore, ribosome crystallographers are on the verge of phasing the diffraction patterns they have had for several years, and near-atomic resolution models for entire ribosomal subunits could emerge from this source at any time. The literature relevant to these developments is reviewed below.
Optimization scheme for the quantum efficiency of GaInN-based green-light-emitting diodes (2006)

We have optimized the internal quantum efficiency (IQE) of GaInN∕GaN quantum-well (QW) structures. For an emission wavelength of 460nm, a high IQE of 73% was achieved. For a longer emission wavelength, calculations predict higher oscillator strength for thinner QWs but higher In content. We observe an improvement in IQE of almost 50% when reducing the QW width from 2.7nmto1.8nm, and increasing the In content for the whole blue to green spectral region with IQE=40% at 525nm. The typical saturation of the output power with increasing current that occurs, particularly for green-light-emitting diodes, is extremely weak in our structures.
Imparting Functionality to Biocatalysts via Embedding Enzymes into Nanoporous Materials by a de Novo Approach: Size-Selective Sheltering of Catalase in Metal–Organic Framework Microcrystals (2015)

We develop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic frameworks (MOFs). The proof-of-concept design is demonstrated by embedding catalase molecules into uniformly sized ZIF-90 crystals via a de novo approach. We have carried out electron microscopy, X-ray diffraction, nitrogen sorption, electrophoresis, thermogravimetric analysis, and confocal microscopy to confirm that the ~10 nm catalase molecules are embedded in 2 μm single-crystalline ZIF-90 crystals with ~5 wt % loading. Because catalase is immobilized and sheltered by the ZIF-90 crystals, the composites show activity in hydrogen peroxide degradation even in the presence of protease proteinase K.
CF-252 Neutron Brachytherapy: An Advance for Bulky Localized Cancer Therapy (1984)

The physical and radiobiogical basis as well as the rationale for neutron brachytherapy, using Cf-252, in human cancer therapy is reviewed. Cf-252 brachytherapy represents an economical and effective form of neutron radiotherapy that is readily and safely applied clinically. It can be used anywhere in the world without unusual personnel, equipment or facilities, or prohibitive expenses or maintenance costs. Used on bulky head and neck, thoracic, abdominal, pelvic, brain and appendage cancers, it overcomes hypoxic radioresistance and produces remarkable rates of tumor clearance. It is easily combined with photon radiotherapy and in proper schedules and doses, it can control advanced but still localized regional cancers to produce tumor cure. It will clear the local manifestations of recurrent or metastatic tumors or advanced stages of primary tumors and therefore in conjunction with other adjuvant therapies offers much more effective tumor control and palliation than present conventional therapy. 208 refs.
Future integrated broadband fiber, wireless, and satellite networks (2006)

With the increasing technical maturity in fiber, wireless and satellite communication technologies, new horizons are becoming feasible for future broadband networks, providing economical data rates well in excess of Gbps for stationary and mobile users as well as novel applications these advanced network services will permit. This talk explores the future architecture possibilities of such a network using new and radical technology building blocks such as: free space laser communications, multiple access multi-beam data satellite communications, novel all-optical network transport/switching and analog transmission and processing over optical carriers that support coherent distributed platform sensing and communications. We will articulate why we have to design this new network across layers from the Physical Layer to the Network and Transport Layers (even the Application Layer). Not only can future network performance and cost undergo quantum-leap improvements; such a network can have profound transforming effects on space and terrestrial system architectures for sensing, healthcare, early warning systems, disaster relief, research collaborations and other new commercial applications.
Captodiamine, a putative antidepressant, enhances hypothalamic BDNF expression in vivo by synergistic 5-HT2c receptor antagonism and sigma-1 receptor agonism (2013)

The putative antidepressant captodiamine is a 5-HT2c receptor antagonist and agonist at sigma-1 and D3 dopamine receptors, exerts an anti-immobility action in the forced swim paradigm, and enhances dopamine turnover in the frontal cortex. Captodiamine has also been found to ameliorate stress-induced anhedonia, reduce the associated elevations of hypothalamic corticotrophin-releasing factor (CRF) and restore the reductions in hypothalamic BDNF expression. Here we demonstrate chronic administration of captodiamine to have no significant effect on hypothalamic CRF expression through sigma-1 receptor agonism; however, both sigma-1 receptor agonism or 5-HT2c receptor antagonism were necessary to enhance BDNF expression. Regulation of BDNF expression by captodiamine was associated with increased phosphorylation of transcription factor CREB and mediated through sigma-1 receptor agonism but blocked by 5-HT2c receptor antagonism. The existence of two separate signalling pathways was confirmed by immunolocalisation of each receptor to distinct cell populations in the paraventricular nucleus of the hypothalamus. Increased BDNF induced by captodiamine was also associated with enhanced expression of synapsin, but not PSD-95, suggesting induction of long-term structural plasticity between hypothalamic synapses. These unique features of captodiamine may contribute to its ability to ameliorate stress-induced anhedonia as the hypothalamus plays a prominent role in regulating HPA axis activity.
Identification of a determinant within the human immunodeficiency virus 1 surface envelope glycoprotein critical for productive infection of primary monocytes. (1991)

Profound differences exist in the replicative capacities of various human immunodeficiency virus 1 isolates in primary human monocytes. To investigate the molecular basis for these differences, recombinant full-length clones were constructed by reciprocal DNA fragment exchange between a molecular clone derived from a monocyte-tropic isolate (ADA) and portions of two full-length clones incapable of infection or replication in primary monocyte cultures (HXB2 and NL4-3). Virions derived from proviral clones that contained ADA sequences encoding vpu and the N and C termini of the surface envelope glycoprotein (gp120) were incapable of replication in monocytes. However, a 283-base-pair ADA sequence encoding amino acids 240-333 of the mature gp120 protein conferred the capacity for high-level virus replication in primary monocytes. The predicted amino acid sequence of this ADA clone differed from NL4-3 and HXB2 at 22 of 94 residues in this portion of gp120, which includes the entire third variable domain. Only 2 of 11 residues implicated in CD4 binding are located in this region of gp120 and are identical in HXB2, NL4-3, and ADA. Alignment of the ADA sequence with published amino acid sequences of three additional monocyte-replicative and three monocyte-nonreplicative clones indicates 6 discrete residues with potential involvement in conferring productive human immunodeficiency virus 1 infection of primary monocytes.
Extremely low nonalloyed and alloyed contact resistance using an InAs cap layer on InGaAs by molecular-beam epitaxy (1988)

Extremely low alloyed and nonalloyed ohmic contact resistances have been formed on n-type InAs/In0.53Ga0.47As/In0.52Al0.48As structures grown on InP(Fe) by molecular-beam epitaxy. To insure the accuracy of the small contact resistances measured, an extended transmission line model was used to extrapolate contact resistances from test patterns with multiple gap spacings varying from 1 to 20 μm. For a 150-Å-thick InAs layer doped to 2×1018 cm−3 and a 0.1-μm-thick InGaAs layer doped to 1×1018 cm−3, a specific contact resistance of 2.6×10−8 Ω* cm2 was measured for the nonalloyed contact, while a resistance less than 1.7×10−8 Ω* cm2 is reported for the alloyed contact. Conventional Au-Ge/Ni/Au was used for the ohmic metal contact and alloying was performed at 500 °C for 50 s in flowing H2. Using a thermionic field emission model, the barrier height at the InAs/InGaAs interface was calculated to be 20 meV.
Calcium, vitamin D, and colon cancer. (1991)

Calcium contributes to the progression of epithelial cells through all phases of the proliferative cycle and into stages of cell differentiation; intracellular concentrations of calcium that are required for cell renewal, however, are lower than those required for epithelial-cell differentiation. These effects of calcium are modulated by interactions with 1,25-dihydroxy-vitamin D3, phosphate, and fatty acids, all of which are partly dependent on dietary intake. In rodent models, increased dietary calcium inhibited hyperproliferation of colon epithelial cells induced by increased levels of fatty acids or bile acids present in the colon. When carcinogens induced hyperproliferation of colon epithelial cells the hyperproliferation was decreased by added dietary calcium, and in several animal models the occurrence of carcinogen-induced carcinomas of the colon decreased with increased dietary calcium. A nutritional stress diet, designed to represent human Western dietary intake of calcium, phosphate, vitamin D, and fat, produced hyperproliferation and hyperplasia in the colons of rodents; these effects were reduced by increasing dietary levels of calcium. Decreased levels of ornithine decarboxylase also were reported in human and rodent colon mucosa exposed to increasing levels of calcium. In human subjects at increased risk for familial colon cancer, hyperproliferation of colon epithelial cells was reduced after oral dietary supplementation with calcium. In epidemiological studies, several investigators reported inverse correlations between levels of dietary calcium intake and the incidence of colon cancer. Extrapolation of the data have suggested a protective effect of total calcium intakes above 1500 to 1800 mg/day.
Structural modulation of self-oscillating gels: changing the proximity of the catalyst to the polymer backbone to tailor chemomechanical oscillation (2012)

We designed and synthesised two new polymerizable ruthenium complexes that catalyse the Belousov–Zhabotinsky (BZ) oscillating reaction and incorporated them into a copolymer to form hydrogels. The periodic oxidation and reduction of the attached ruthenium complex in the BZ reaction induces hydrating and dehydrating effects, respectively, that result in self-oscillatory volume changes of the hydrogel. We evaluated the correlation between the chemomechanical oscillation properties of the hydrogel and the proximity of the catalyst to the polymer backbone. Our results indicate that, like the change of such macroscopic parameters as temperature, reactant concentrations and pH, varying the microscopic distance between the catalyst and the polymeric chain provides a new way to tailor the chemomechanical behaviour, e.g., the initiation time, the frequency of oscillation, and the volume change of BZ hydrogels. Moreover, variation of the catalysts offers a new means to control the microstructure of the copolymer by expanding the range of monomer ratios. Modulation of molecular structure appears to be an effective way to alter the reaction–diffusion profile of species within heterogeneous chemoresponsive gels, thus contributing to the development of multifunctional, active soft materials capable of converting chemical energy into controllable mechanical forces.
The simultaneous blockade of chemokine receptors CCR2, CCR5 and CXCR3 by a non-peptide chemokine receptor antagonist protects mice from dextran sodium sulfate-mediated colitis (2005)

Chemokine receptors CCR2, CCR5 and CXCR3 are involved in the regulation of macrophage- and T cell-mediated immune responses and in the migration and activation of these cells. In order to determine whether blockade of these chemokine receptors modulates intestinal inflammation, we investigated here the effect of a non-peptide chemokine receptor antagonist, TAK-779 (N,N-dimethyl-N-[4-[[[2-(4-methylphenyl)-6,7-dihydro-5H-benzocyclohepten-8-yl]carbonyl]amino]benzyl]-tetrahydro-2H-pyran-4-aminium chloride), in mice with dextran sodium sulfate (DSS)-induced experimental colitis. C57BL/6 mice were fed 5% DSS in their drinking water for up to 7 days with or without the administration of TAK-779. The severity of inflammation in the colon was assessed by clinical signs and histological examination. Infiltration of inflammatory cells into the mucosa was analyzed by immunohistochemistry, and the expression of cytokine and chemokine mRNAs in tissues was quantitated by reverse transcription-PCR. During DSS-induced colitis, the recruitment of monocytes/macrophages into the colonic mucosa and the induction of proinflammatory cytokines correlated with the severity of intestinal inflammation. The onset of clinical signs and histopathologic features were delayed in animals treated with TAK-779. The expression of CCR2, CCR5 and CXCR3 mRNAs was inhibited in the TAK-779-treated mice. Consistent with these results, infiltration of monocytes/macrophages into the lamina propria was almost completely inhibited and the expression of colonic IL-1beta and IL-6 was significantly decreased in the TAK-779-treated mice. The blockade of CCR2, CCR5 and CXCR3 prevents murine experimental colitis by inhibiting the recruitment of inflammatory cells into the mucosa. Therefore, chemokines and their receptors may be therapeutic targets for the treatment of inflammatory bowel disease.
Contribution of conformational stability and reversibility of unfolding to the increased thermostability of human and bovine superoxide dismutase mutated at free cysteines. (1990)

The conformational stability and reversibility of unfolding of the human dimeric enzyme Cu Zn superoxide dismutase (HSOD) and the three mutant enzymes constructed by replacement of Cys6 by Ala and Cys111 by Ser, singly and in combination, were determined by differential scanning calorimetry. The differential scanning calorimetry profile of wild-type HSOD consists of two components, which probably represent the unfolding of the oxidized and reduced forms of the enzyme, with denaturation temperatures (Tm) of 74.9 and 83.6 degrees C, approximately 7 degrees lower than those for bovine superoxide dismutase (BSOD). The conformational stabilities of the two components of the mutant HSOD's differ only slightly from those of the wild type (delta delta Gs of -0.2 to +0.8 kcal/mol of dimer), while replacement of the BSOD Cys6 by Ala is somewhat destabilizing (delta delta G of -0.7 to -1.3 kcal/mol of dimer). These small alterations in conformational stability do not correlate with the large increases in resistance to thermal inactivation following substitution of free Cys in both HSOD and BSOD (McRee, D.E., Redford, S.M., Getzoff, E.D., Lepock, J.R., Hallewell, R.A., and Tainer, J.A. (1990) J. Biol. Chem. 265, 14234-14241 and Hallewell, R.A., Imlay, K.C., Laria, I., Gallegos, C., Fong, N., Irvine, B., Getzoff, E.D., Tainer, J.A., Cubelli, D.E., Bielski, B.H.J., Olson, P., Mallenbach, G.T., and Cousens, L.S. (1991) Proteins Struct. Funct. Genet., submitted for publication). The reversibility of unfolding was determined by scanning part way through the profile, cooling, rescanning, and calculating the amount of protein irreversibly unfolded by the first scan. The order of reversibility at a constant level of unfolding is the same as the order of resistance to inactivation for both the HSOD and BSOD wild-type and mutant enzymes. Thus, the greater resistance to thermal inactivation of the superoxide dismutase enzymes with free Cys replaced by Ala or Ser is dominated by a greater resistance to irreversible unfolding and relatively unaffected by changes in conformational stability.
Tubulin as a target for anticancer drugs: Agents which interact with the mitotic spindle (1998)

Tubulin is the biochemical target for several clinically used anticancer drugs, including paclitaxel and the vinca alkaloids vincristine and vinblastine. This review describes both the natural and synthetic agents which are known to interact with tubulin. Syntheses of the more complex agents are referenced and the potential clinical use of the compounds is discussed. This review describes the biochemistry of tubulin, microtubules, and the mitotic spindle. The agents are discussed in relation to the type of binding site on the protein with which they interact. These are the colchicine, vinca alkaloid, rhizoxin/maytansine, and tubulin sulfhydryl binding sites. Also included are the agents which either bind at other sites or unknown sites on tubulin. The literature is reviewed up to October 1997.
Effect of Atrial Natriuretic Peptide on Ischemic Brain Edema: Changes in Brain Water and Electrolytes (1990)

The effects of intraventricularly administered atrial natriuretic peptide (ANP) on the brain water, sodium, and potassium contents in ischemic brain edema were investigated. By use of a three-vessel occlusion model, ischemic brain edema was produced in the rat brain by 15 minutes of global ischemia followed by recirculation. Water content was measured by means of a drying/weighing method; sodium and potassium contents were measured by means of flame photometry. The effects of intraventricular administration of ANP were evaluated by a comparison between the groups given 2 and 5 μg of atriopeptin II (treated) and those given 0.9% NaC1 (sham-treated). The treated groups showed significant decreases in brain water (P < 0.02) and sodium (P < 0.01) contents at 15 and 30 minutes after recirculation, whereas the brain potassium contents remained unaltered. Before ischemia and immediately after 15 minutes of ischemia, intraventricularly administered ANP did not significantly change the brain water, sodium, or potassium contents. There was no significant difference in the effect on the amount of brain water and sodium between the two doses (2 and 5 μg). These effects of ANP were thought not to be mediated by primary changes in serum osmolality and sodium and potassium concentrations, because intraventricular administration of ANP did not change them significantly. The present results reveal that, in ischemic brain edema. ANP may act directly on the central nervous system to inhibit brain water and sodium accumulation. (Neurosurgery 27:39-44, 1990)
Transmission of 640-Gb/s RZ-OOK Channel Over 100-km SSMF by Wavelength-Transparent Conjugation (2011)

We report the first demonstration of 640-Gb/s return-to-zero ON-OFF keying channel transmission over a 100-km standard single-mode fiber link employing midspan phase conjugation. A frequency-degenerate conjugate field spanning more than 20 nm is created in a low-birefringence parametric mixer for the first time. Physical separation of the conjugate field from the original field is enabled by utilizing pump polarization nondegeneracy. Rigorous link characterization using a high-quality 640-Gb/s transmitter and a high-sensitivity receiver revealed error-free (bit error ratio <;10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-9</sup> ) performance, eliminating the need for impractical fiber length control or electronic signal processing. The compatibility of wavelength-transparent conjugation with spectrally inefficient channel implies that channels with higher bit rates and better spectral efficiency can also be compensated.
A 900-MHz/1.8-GHz CMOS receiver for dual-band applications (1998)

A dual-band receiver employs the Weaver architecture with two tuned radio-frequency stages and a common intermediate-frequency stage to allow operation with 900-MW and 1.8-GHz standards while using only two oscillators. Fabricated in a digital 0.6-/spl mu/m CMOS technology, the receiver achieves an overall noise figure of 4.7 dB and input third intercept point of -8 dBm at 900 MHz, and 4.9 dB and -6 dBm at 1.8 GHz. The voltage gain is 23 dB with a power dissipation of 75 mW from a 3-V supply.
Delta-Sleep-Inducing Peptide Stimulates Melatonin, 5-Methoxytryptophol and Serotonin Secretion from Perifused Rat Pineal Glands (1992)

The pineal gland is known to synthesize numerous indolamines. Since delta-sleep-inducing peptide (DSIP, a tryptophan nonapeptide) is found in the pineal gland, its effect on the secretion of indolamines was investigated. DSIP stimulated melatonin (MEL), 5-methoxytryptophol (5-ML) and serotonin (5-HT) synthesis and release, whereas it did not affect pineal cyclic AMP levels. The stimulatory effect of DSIP on MEL secretion was dose dependent between 5 x 10(-6) and 10(-4) M, whereas the minimal effective concentration of DSIP on 5-ML secretion was higher than 10(-5) M. The effect of DSIP (10(-4) M) was compared to the effect of isoproterenol (ISO, 10(-6) M) on MEL and 5-HT release. ISO stimulated MEL secretion and concomitantly decreased 5-HT release. With regard to kinetic characteristics, the effect of DSIP (10(-4) M) on MEL release was faster and of shorter duration than the effect of ISO (10(-6) M; 2 and 4 h, respectively). At 10(-4) M, DSIP potentiated the ISO-induced increase of MEL secretion. The DSIP-stimulated release of MEL was not significantly altered when the pineal glands were treated with 10(-5) M propranolol (a beta-adrenergic antagonist), 10(-5) M prazosin (an alpha 1-adrenergic antagonist) or 10(-5) M naloxone (an opioidergic antagonist). This study demonstrates that the DSIP-induced secretion of indolamines from rat pineal glands may not be elicited through the well-known noradrenergic or opioid systems.
On the convergence of Volterra filter equalizers using a pth-order inverse approach (2001)

The pth-order inverse method is one of important approaches to Volterra equalization. However, when a pth-order Volterra equalizer instead of an exact Volterra equalizer is connected in cascade before (after) a nonlinear system, the existence of Volterra filter equalization and the approximation output error bound of the resulting system have yet to be reported. In this paper, the concept of local l/sup 2/ stability for a Volterra system is introduced, and the algorithmic formulae of a pth-order inverse equalizer via a multidimension z-transform are presented. The output error signal and the approximation output error bound of the resulting system are investigated as well. It is shown that the approximation output error tends to zero as p tends to infinity for a finite range of input amplitude values. Finally some simulation results are presented and discussed.