gebhart/patrepeval huggingface dataset:
Specific Sequestering Agents for the Actinides. 29. Stability of the Thorium(IV) Complexes of Desferrioxamine B (DFO) and Three Octadentate Catecholate or Hydroxypyridinonate DFO Derivatives:  DFOMTA, DFOCAMC, and DFO-1,2-HOPO. Comparative Stability of the Plutonium(IV) DFOMTA Complex1 (1996)

The metal complex stability constants of Th(IV) with desferrioxamine B (DFO) and three octadentate derivatives [N-(2,3-dihydroxy-4-carboxybenzoyl)desferrioxamine B (DFOCAMC), N-(1,2-dihydro-1-hydroxy-2-oxopyridin-6-yl)carbonyl)desferrioxamine B (DFO-1,2-HOPO) and N-(2,3-dihydroxy-4-(methylamido)benzoyl)desferrioxamine B (DFOMTA)] have been determined. The formation constant of the Pu(IV)/DFOMTA complex has also been determined, and the formation constants have been estimated for the other Pu(IV) complexes of octadentate DFO derivatives. The DFO derivatives form 1:1 complexes with Th(IV) in aqueous solution. The solution chemistry of the Th(IV) complexes has been studied by spectrophotometric, potentiometric and proton NMR titrations. The Th(IV) formation constants are as follows (log K(f) values and esd's): DFO, 26.6(1); DFOMTA, 38.55(5); DFOCAMC, 37.2(3); DFO-1,2-HOPO, 33.7(4). The Pu(IV)/DFOMTA formation constant, determined by competitive spectrophotometric titration is (log K(f) value) 41.7(2). The estimation of the other Pu(IV) formation constants are as follows (log K(f) values): DFOCAMC, 40.4; DFO-1,2-HOPO, 36.9. The selectivity of DFO and the three derivatives for actinide(IV) ions is discussed.
Molecular and Cellular Requirements for Enhanced Antigen Cross-Presentation to CD8 Cytotoxic T Lymphocytes (2007)

MHC class I-mediated cross-priming of CD8 T cells by APCs is critical for CTL-based immunity to viral infections and tumors. We have shown previously that tumor-secreted heat shock protein gp96-chaperoned peptides cross prime CD8 CTL that are specific for genuine tumor Ags and for the surrogate Ag OVA. We now show that tumor-secreted heat shock protein gp96-chaperoned peptides enhance the efficiency of Ag cross-priming of CD8 CTL by several million-fold over the cross-priming activity of unchaperoned protein alone. Gp96 also acts as adjuvant for cross-priming by unchaperoned proteins, but in this capacity gp96 is 1000-fold less active than as a peptide chaperone. Mechanistically, the in situ secretion of gp96-Ig by transfected tumor cells recruits and activates dendritic cells and NK cells to the site of gp96 release and promotes CD8 CTL expansion locally. Gp96-mediated cross-priming of CD8 T cells requires B7.1/2 costimulation but proceeds unimpeded in lymph node-deficient mice, in the absence of NKT and CD4 cells and without CD40L. Gp96-driven MHC I cross-priming of CD8 CTL in the absence of lymph nodes provides a novel mechanism for local, tissue-based CTL generation at the site of gp96 release. This pathway may constitute a critically important, early detection, and rapid response mechanism that is operative in parenchymal tissues for effective defense against tissue damaging antigenic agents.
Tetracyanoethylene Oxide (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTetracyanoethylene OxideW. J. Linn, O. W. Webster, and R. E. BensonCite this: J. Am. Chem. Soc. 1963, 85, 13, 2032–2033Publication Date (Print):July 1, 1963Publication History Published online1 May 2002Published inissue 1 July 1963https://pubs.acs.org/doi/10.1021/ja00896a041https://doi.org/10.1021/ja00896a041research-articleACS PublicationsRequest reuse permissionsArticle Views264Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Vibration Based Condition Monitoring: A Review (2004)

Vibration based condition monitoring refers to the use of in situ non-destructive sensing and analysis of system characteristics –in the time, frequency or modal domains –for the purpose of detecting changes, which may indicate damage or degradation. In the field of civil engineering, monitoring systems have the potential to facilitate the more economical management and maintenance of modern infrastructure. This paper reviews the state of the art in vibration based condition monitoring with particular emphasis on structural engineering applications.
Hospital-wide Code Rates and Mortality Before and After Implementation of a Rapid Response Team (2008)

In this large single-institution study, rapid response team implementation was not associated with reductions in hospital-wide code rates or mortality.
Laulimalide and isolaulimalide, new paclitaxel-like microtubule-stabilizing agents. (1999)

A mechanism-based screening program aimed at the discovery of new antimicrotubule agents from natural products yielded laulimalide and isolaulimalide, two compounds with paclitaxel-like microtubule-stabilizing activity. Treatment of A-10 cells with laulimalide resulted in a dose-dependent reorganization of the cellular microtubule network and the formation of microtubule bundles and abnormal mitotic spindles. Coincident with the microtubule changes, these two compounds induced nuclear convolution and the formation of multiple micronuclei. Laulimalide is a potent inhibitor of cellular proliferation with IC50 values in the low nanomolar range, whereas isolaulimalide is much less potent with IC50 values in the low micromolar range. In contrast to paclitaxel, both laulimalide and isolaulimalide inhibited the proliferation of SKVLB-1 cells, a P-glycoprotein overexpressing multidrug-resistant cell line, suggesting that they are poor substrates for transport by P-glycoprotein. Incubation of MDA-MB-435 cells with laulimalide resulted in mitotic arrest and activation of the caspase cascade of proteolytic enzymes that accompany apoptotic cell death. Laulimalide stimulated tubulin polymerization and, although less potent than paclitaxel, it was more effective. Laulimalide-induced tubulin polymers resembled paclitaxel-induced polymers, although the laulimalide-induced polymers appeared notably longer. Laulimalide and isolaulimalide represent a new class of microtubule-stabilizing agents with activities that may provide therapeutic utility.
RUNX3 Is Frequently Inactivated by Dual Mechanisms of Protein Mislocalization and Promoter Hypermethylation in Breast Cancer (2006)

A tumor suppressor function has been attributed to RUNX3, a member of the RUNX family of transcription factors. Here, we examined alterations in the expression of three members, RUNX1, RUNX2, and RUNX3, and their interacting partner, CBF-beta, in breast cancer. Among them, RUNX3 was consistently underexpressed in breast cancer cell lines and primary tumors. Fifty percent of the breast cancer cell lines (n = 19) showed hypermethylation at the promoter region and displayed significantly lower levels of RUNX3 mRNA expression (P < 0.0001) and protein (P < 0.001). In primary Singaporean breast cancers, 9 of 44 specimens showed undetectable levels of RUNX3 by immunohistochemistry. In 35 of 44 tumors, however, low levels of RUNX3 protein were present. Remarkably, in each case, protein was mislocalized to the cytoplasm. In primary tumors, hypermethylation of RUNX3 was observed in 23 of 44 cases (52%) and was undetectable in matched adjacent normal breast epithelium. Mislocalization of the protein, with or without methylation, seems to account for RUNX3 inactivation in the vast majority of the tumors. In in vitro and in vivo assays, RUNX3 behaved as a growth suppressor in breast cancer cells. Stable expression of RUNX3 in MDA-MB-231 breast cancer cells led to a more cuboidal phenotype, significantly reduced invasiveness in Matrigel invasion assays, and suppressed tumor formation in immunodeficient mice. This study provides biological and mechanistic insights into RUNX3 as the key member of the family that plays a role in breast cancer. Frequent protein mislocalization and methylation could render RUNX3 a valuable marker for early detection and risk assessment.
Low-Cost Optical Indoor Localization System for Mobile Objects without Image Processing (2006)

While being very successful in everyday life, GPS-based localization systems exhibit limited performance under trees, behind walls, and in closed rooms, and sometimes induce costs that are way too high to be acceptable for some applications. This paper addresses these problems by exploring an alternative localization system, which is based on image processing and does most of the processing in analog. Because of its low-cost and low-energy properties, this system might also be interesting for other application areas, such as sensor networks.
Hydroxyethylene isostere inhibitors of human immunodeficiency virus-1 protease: structure-activity analysis using enzyme kinetics, x-ray crystallography, and infected T-cell assays (1992)

Analogues of peptides ranging in size from three to six amino acids and containing the hydroxyethylene dipeptide isosteres Phe psi Gly, Phe psi Ala, Phe psi NorVal, Phe psi Leu, and Phe psi Phe, where psi denotes replacement of CONH by (S)-CH(OH)CH2, were synthesized and studied as HIV-1 protease inhibitors. Inhibition constants (Ki) with purified HIV-1 protease depend strongly on the isostere in the order Phe psi Gly greater than Phe psi Ala greater than Phe psi NorVal greater than Phe psi Leu greater than Phe psi Phe and decrease with increasing length of the peptide analogue, converging to a value of 0.4 nM. Ki values are progressively less dependent on inhibitor length as the size of the P1' side chain within the isostere increases. The structures of HIV-1 protease complexed with the inhibitors Ala-Ala-X-Val-Val-OMe, where X is Phe psi Gly, Phe psi Ala, Phe psi NorVal, and Phe psi Phe, have been determined by X-ray crystallography (resolution 2.3-3.2 A). The crystals exhibit symmetry consistent with space group P6(1) with strong noncrystallographic 2-fold symmetry, and the inhibitors all exhibit 2-fold disorder. The inhibitors bind in similar conformations, forming conserved hydrogen bonds with the enzyme. The Phe psi Gly inhibitor adopts an altered conformation that places its P3' valine side chain partially in the hydrophobic S1' pocket, thus suggesting an explanation for the greater dependence of the Ki value on inhibitor length in the Phe psi Gly series. From the kinetic and crystallographic data, a minimal inhibitor model for tight-binding inhibition is derived in which the enzyme subsites S2-S2' are optimally occupied. The Ki values for several compounds are compared with their potencies as inhibitors of proteolytic processing in T-cell cultures chronically infected with HIV-1 (MIC values) and as inhibitors of acute infectivity (IC50 values). There is a rank-order correspondence, but a 20-1000-fold difference, between the values of Ki and those of MIC or IC50. IC50 values can approach those of Ki but are highly dependent on the conditions of the acute infectivity assay and are influenced by physiochemical properties of the inhibitors such as solubility.
An architecture for embedded software integration using reusable components (2000)

Article An architecture for embedded software integration using reusable components Share on Authors: Shige Wang Real-Time Computing Laboratory, Department of Electrical Engineering and Computer Science, The University of Michigan, 1301 Beal Avenue, Ann Arbor, MI Real-Time Computing Laboratory, Department of Electrical Engineering and Computer Science, The University of Michigan, 1301 Beal Avenue, Ann Arbor, MIView Profile , Kang G. Shin Real-Time Computing Laboratory, Department of Electrical Engineering and Computer Science, The University of Michigan, 1301 Beal Avenue, Ann Arbor, MI Real-Time Computing Laboratory, Department of Electrical Engineering and Computer Science, The University of Michigan, 1301 Beal Avenue, Ann Arbor, MIView Profile Authors Info & Claims CASES '00: Proceedings of the 2000 international conference on Compilers, architecture, and synthesis for embedded systemsNovember 2000 Pages 110–118https://doi.org/10.1145/354880.354896Online:01 November 2000Publication History 25citation2,062DownloadsMetricsTotal Citations25Total Downloads2,062Last 12 Months10Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
High rep-rate inductive-energy-storage pulsed power modulator using high voltage static induction thyristor (2003)

A high voltage static induction thyristor (SI Thy) has two significant features. One is that carriers can be quickly pulled out of gate region without applying a high voltage to the gate. The other is that its ON-state voltage is very low. These features make the SI Thy especially suitable for a switch of high rep-rate inductive-energy-storage pulsed power modulators, since the switch can interrupt a current rapidly and energy loss by the switch in a conduction phase can be remarkably reduced. In this research, a high rep-rate inductive-energy-storage pulsed power modulator, which uses a high voltage SI Thy as an opening switch, has been designed and constructed for application to an induction synchrotron. The maximum operating frequency of the modulator is expected to be 1 MHz. The induction synchrotron accelerates bunched charged particle beams using induced electric fields, which is proportional to the time derivative of magnetic flux in a toroidal core. In order to prevent a magnetic flux of the core from saturating, it is necessary to reset the toroidal core after each acceleration pulse. Therefore, a reset circuit independent of an acceleration circuit is needed in general. However, the proposed modulator can supply both the acceleration and the reset pulses without any additional circuit and is especially suitable for an induction synchrotron accelerator.
Intrauterine effects of electromagnetic fields?(low frequency, mid-frequency RF, and microwave): Review of epidemiologic studies (1999)

Electromagnetic radiations are named according to frequency or to wavelength (which is inversely proportional to frequency) and create electromagnetic fields (EMFs). Frequencies widely vary according to sources: high-voltage power lines, electrically heated beds, MRI, VDTs, microwave ovens, satellite, and radio/TV transmissions or cellular phone transmitters/receivers. Public concern has increased about the potential health effects of EMFs. There are arguments in favour of EMFs being biologically active, but no mechanism has been identified that explains the link between EMFs and bioeffects. Human data reviewed concern the potential reproductive effects (mainly spontaneous abortions, low birthweight and congenital malformations) of exposure to sources of EMFs: maternal residence, electrically heated beds, occupational exposure (mainly video display terminals), and medical exposures. The available epidemiologic studies all have limitations that prevent to draw clearcut conclusions on the effects of EMFs on human reproduction. EMFs are ubiquitous and unavoidable exposures. The matter of possible effects cannot be considered closed, but until our understanding of the biologic important parameters of EMFs exposures is stronger,design of new studies will be difficult and small epidemiologic studies are unlikely to provide definitive answers and should not be given high priority. No conclusion can be drawn for radiofrequencies and microwaves because of lack of data. There is no convincing evidence today that EMFs of the sort pregnant women or potential fathers meet in occupational or daily life exposures does any harm to the human reproductive process.
The power and pitfalls of IL-12 (2012)

In this issue of Blood, Pegram and colleagues describe the ability to improve adoptive T-cell therapies by engineering T cells to secrete the potent proinflammatory cytokine IL-12.1 These exciting findings significantly extend previous observations made in a murine melanoma model targeting naturally occurring tumor antigens.2,3
Role of the HIV gp120 Conserved Domain 5 in Processing and Viral Entry (2008)

The importance of the HIV gp120 conserved domain 5 (gp120-C5) to envelope function has been examined by alanine scanning mutagenesis and subsequent characterization of the mutagenic effects on viral entry and envelope expression, processing, and incorporation, as well as gp120 association with gp41. With respect to the wild-type gp120, mutational effects on viral entry fall into three classes: (1) functional (V489A, E492A, P493A, T499A, K500A, K502A, R503A, R504A, V505A, and V506A; (2) nonfunctional (I491A, L494A, V496A, and P498A); (3) enhanced (K490A, G495A, and Q507A). The nonfunctionality of the mutants is attributed to a combination of deleterious effects on processing, gp120-gp41 association, and membrane fusion. In the case of the nonfunctional mutant P498A, the introduction of the SOS mutation (A501C/T601C) results in substantially increased envelope processing and a gain of function. The effects of the mutants are interpreted with respect to the structures of gp41 and gp120. The extent of sensitivity of gp120-C5 to alanine substitutions underscores the importance of this domain to envelope function and suggests that gp120-C5 is an attractive and novel target for future drug discovery efforts.
Studies in the synthesis of camptothecin. An efficient synthesis of 2,3-dihydro-1H-pyrrolo[3,4-b]quinoline (1971)

An efficient regioselective synthesis of 2,3-dihydro-1H-pyrrolo[3,4-b]quinoline (3), a key intermediate in the synthesis of camptothecin, has been developed. The synthesis, which has been extended to a number of analogues, involves an acid-catalysed Friedländer condensation in the absence of a solvent. Base-catalysed condensation was shown to lead predominantly to the isomeric 2,3-dihydro-1H-pyrrolo[3,2-b]quinoline (10). The relative stabilities of the enols and enolates derived from N-acetyl-3-pyrrolidone and ethyl 3-oxopyrrolidine-1-carboxylate, intermediates in the synthesis of compounds (3) and (10), are discussed.
The formation of rhizopterin and formylated folate polyglutamates during the growth of Staphylococcus epidermidis (1972)

The distribution of various folate forms during the growth of Staphylococcus epidermidis is described. Cellular-free folate activity (pteroate-like substances and mono- and di-glutamate folates) was quite prevalent in the early and mid-log phase of growth, but subsequently reached a minimal level in the stationary phase of growth. This free folate activity was identified as rhizopterin, with an extremely small amount of folic acid per se also being found. Cellular polyglutamate folates (folate conjugates with three or more glutamic acid units) were found in relatively high amounts throughout growth, and for any cellular sample constituted most of the total folate activity. The polyglutamate folate compounds were identified as formylated derivatives. A biosynthetic pathway from rhizopterin to the formylated polyglutamate folates is proposed.
Synthesis and complexation properties of a water-soluble optically active cyclophane incorporating a 4-naphthyl-1,2,3,4-tetrahydroisoquinoline unit as a chiral spacer (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and complexation properties of a water-soluble optically active cyclophane incorporating a 4-naphthyl-1,2,3,4-tetrahydroisoquinoline unit as a chiral spacerTaxiarchis M. Georgiadis, Millie M. Georgiadis, and Francois DiederichCite this: J. Org. Chem. 1991, 56, 10, 3362–3369Publication Date (Print):May 1, 1991Publication History Published online1 May 2002Published inissue 1 May 1991https://pubs.acs.org/doi/10.1021/jo00010a034https://doi.org/10.1021/jo00010a034research-articleACS PublicationsRequest reuse permissionsArticle Views291Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Rapid and Sensitive RT-QuIC Detection of Human Creutzfeldt-Jakob Disease Using Cerebrospinal Fluid (2015)

A long-standing problem in dealing with various neurodegenerative protein misfolding diseases is early and accurate diagnosis. This issue is particularly important with human prion diseases, such as CJD, because prions are deadly, transmissible, and unusually resistant to decontamination. The recently developed RT-QuIC test allows for highly sensitive and specific detection of CJD in human cerebrospinal fluid and is being broadly implemented as a key diagnostic tool. However, as currently applied, RT-QuIC takes 2.5 to 5 days and misses 11 to 23% of CJD cases. Now, we have markedly improved RT-QuIC analysis of human CSF such that CJD and non-CJD patients can be discriminated in a matter of hours rather than days with enhanced sensitivity. These improvements should allow for much faster, more accurate, and practical testing for CJD. In broader terms, our study provides a prototype for tests for misfolded protein aggregates that cause many important amyloid diseases, such as Alzheimer's, Parkinson's, and tauopathies.