gebhart/patrepeval huggingface dataset:
Differential Conjugation of Tat Peptide to Superparamagnetic Nanoparticles and Its Effect on Cellular Uptake (2002)

Surface modification of superparamagnetic contrast agents with HIV-1 tat peptide has emerged as a promising means for intracellular magnetic labeling and noninvasive tracking of a large number of cell types with MRI. To achieve efficient intracellular delivery of the nanoparticles, we investigated the effect on cellular uptake of superparamagnetic iron oxide particles by varying the number of attached tat peptides. First, we report here a modified P2T method in measuring the numbers of surface attachments per particle through disulfide linkage. The method was shown to have desirable simplicity and reproducibility. With the P2T method as a tool, conjugates with progressively higher ratios of peptide-to-particle were synthesized. We were able to demonstrate that higher numbers of tat peptide facilitate the cellular uptake of iron oxide nanoparticles in a nonlinear fashion. Cells labeled with these optimized preparations were readily detectable by MR imaging. The increase in sensitivity could allow in vivo tracking of 100-fold lower cell concentration than currently described.
Immunization of complex networks (2002)

Complex networks such as the sexual partnership web or the Internet often show a high degree of redundancy and heterogeneity in their connectivity properties. This peculiar connectivity provides an ideal environment for the spreading of infective agents. Here we show that the random uniform immunization of individuals does not lead to the eradication of infections in all complex networks. Namely, networks with scale-free properties do not acquire global immunity from major epidemic outbreaks even in the presence of unrealistically high densities of randomly immunized individuals. The absence of any critical immunization threshold is due to the unbounded connectivity fluctuations of scale-free networks. Successful immunization strategies can be developed only by taking into account the inhomogeneous connectivity properties of scale-free networks. In particular, targeted immunization schemes, based on the nodes' connectivity hierarchy, sharply lower the network's vulnerability to epidemic attacks.
Biosynthesis of ethylene. 4-Methylmercapto-2-oxobutyric acid, an intermediate in the formation from methionine (1969)

The enzyme responsible for the conversion of methionine into a precursor of ethylene in cauliflower florets is a transaminase. The formation of 4-methyl-mercapto-2-oxobutyric acid by this enzyme has been shown. The oxo acid stimulates the synthesis of ethylene when added to floret tissue, and tracer experiments have shown that (14)C is incorporated into ethylene from the labelled oxo acid. The evidence is consistent with the view that the oxo acid is an intermediate in the formation of ethylene from methionine.
SAR650984, A Novel Humanized CD38-Targeting Antibody, Demonstrates Potent Antitumor Activity in Models of Multiple Myeloma and Other CD38+ Hematologic Malignancies (2014)

These results validate CD38 as a therapeutic target and support the current evaluation of this unique CD38-targeting functional antibody in phase I clinical trials in patients with CD38+ B-cell malignancies.
Fasting Induces Hyperlipidemia in Mice Overexpressing Proprotein Convertase Subtilisin Kexin Type 9: Lack of Modulation of Very-Low-Density Lipoprotein Hepatic Output by the Low-Density Lipoprotein Receptor (2006)

Several proprotein convertase subtilisin kexin type 9 (PCSK9) mutations lead to familial hypercholesterolemia by virtue of its role as a negative modulator of the low-density lipoprotein receptor (LDLr). Here, we uncover that upon dietary challenge, the down-regulation of the LDLr is also a key mechanism by which PCSK9 modulates the hepatic production of apolipoprotein-B-containing lipoproteins. Thus, adenoviral-mediated overexpression of PCSK9 in 24-h fasted mice results in massive hyperlipidemia, due to a striking increase in very-low-density lipoprotein (VLDL) triglycerides and apolipoprotein B100 hepatic output. Similar studies in LDLr (-/-) mice demonstrate that PCSK9-mediated alteration of VLDL output in the fasted state requires the LDLr. This increased production of VLDL was associated with a concomitant reduction of intrahepatic lipid stores as well as a lack of down-regulation of peroxisome proliferator-activated receptor-alpha activity and target genes expression. Finally, we show that PCSK9 hepatic expression is inhibited by the hypotriglyceridemic peroxisome proliferator-activated receptor-alpha agonist fenofibrate. In summary, the negative modulation of LDLr expression by PCSK9, which decreases plasma LDL clearance, also promotes an overproduction of nascent VLDL in vivo upon fasting.
MINIATURE ULTRA-LOW-POWER BIOPOTENTIAL AMPLIFIER FOR POTABLE APPLICATIONS (2005)

A high input impedance, high common mode rejection ratio, fixed gain (×100) amplifier is proposed for recording biopotential signals. This miniature amplifier affords the feature of power saving. It can continuously function for as long as 3 months with a small battery (3.3 V, 2.2 g). A practical application of this amplifier for ECG recording has shown that it has great potential for recording other biomedical signals. Hence, this amplifier can be used as a building block at the front end of most biomedical systems. Detailed design considerations and circuit implementation of this amplifier are described to facilitate its acceptance as a common module for this purpose.
CpG DNA as a Th1 Trigger (2000)

Over the last few years, it has been recognized that along with structural components and products of bacteria, bacterial DNA is also capable of signaling infectious danger to cells of the innate immune system. Particular DNA sequences (CpG motifs), which are abundant in prokaryotic (bacterial) but not in mammalian DNA, cause the activation and stimulation of immune cells. Research has been catalyzed by the finding that certain synthetic oligodeoxynucleotides mimic the action of bacterial DNA. Immunostimulation induced by bacterial DNA or synthetic oligonucleotides not only contributes to our knowledge of the pathogen-host interrelationship during infection, but can also be used therapeutically to condition or modify ongoing immune responses of the adaptive immune system. Accordingly, CpG motifs have been used as vaccine adjuvants as well as instructing agents to selectively induce Th1-dominated immune responses. Hence, CpG motifs might be used in the future as adjuvants and/or immunomodulatory agents to treat or prevent undesired Th2-dominated immune responses, such as allergy.
Synthesis of 5a,11b-propanonaphtho[1,2-e][1,2]oxazepines as potential opioid analgesics (1994)

5a, 11b-Propanonaphtho[1,2-e][1,2]oxazepines have been prepared from dihydro-7,14-cyclocodeinone by the following reaction path: nucleophilic cyclopropane-ring opening, N-oxide formation, Cope reaction and intramolecular O-alkylation. The skeleton of the resulting compounds which have potential as analgesics, is closely similar to that of the parent morphinan, although there is a novel annulation of the nitrogen-containing ring which is further expanded to an oxazepine.
Streptavidin as a Macroinitiator for Polymerization:  In Situ Protein−Polymer Conjugate Formation (2005)

This communication reports the first example of polymerization initiated from specific domains on proteins. Streptavidin was coupled with a biotinylated initiator for atom transfer radical polymerization (ATRP) and exposed to an aqueous solution of CuBr/2,2'-bipyridine and monomer. N-Isopropylacrylamide (NIPAAm) and poly(ethylene glycol) methyl ether methacrylate (PEGMA) were readily initiated by the modified streptavidin and polymerized from the protein at room temperature. Formation of streptavidin-polymer conjugates was confirmed by size exclusion chromatography (SEC) and gel electrophoresis. Polymer identity and biotinylation was verified using 1H NMR spectroscopy, gel permeation chromatography (GPC), and surface plasmon resonance (SPR) after dissociation of the biotin-streptavidin complex. This general approach is likely to be extended to other proteins and monomers and promises to enable easy synthesis and purification of a variety of polymer-protein conjugates.
Gene Structure in Eukaryotic Microbes (1987)

The structure and expression of nuclear genes in Saccharomyces Spicing of mRNAS precursors in yeast Mitochondrial genes of the budding yeast Saccharomyces Mitochondrial genes of the fission yeast Schizosaccharomyces The structure and organization of nuclear genes of filamentous fungi The mitochondrial genes of filamentous fungi Gene structure and organization in Physarum polycephalum Gene structure in algae Structure and expression of genes in ciliated protozoa
An extended primer set for PCR amplification of murine kappa variable regions. (1993)

The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irrelevant SP2/0 from the sequence of interest are also provided.
Expert systems in real estate valuation (1990)

Discusses the development of expert systems (ES) as an improvement on computer‐aided valuation techniques (CAV), due to its allowing the modelling of complex non‐linear and qualitative relationships and processes, such as those which exist within the field of valuation. Explores valuation as a set of processes and details current technology with reference to the impact of CAVs in Australia. Discusses expert systems as the latest stage in the evolution of computer methods which support humans in decision making, outlines ES procedure and gives examples of current applications. Considers the application of expert systems to rating valuation and reviews the theory of ′information chunking.
Hybrid systems and the design of embedded controllers for automotive engine management (2002)

The problem of designing an automotive engine control unit for the next-generation automobiles has been formulated as a hybrid control problem. We present first the overall methodology and its basic components. Then we focus on the highest levels of abstraction that involve the formulation of the control problem and its solution. Two particular control sub-problems (cut-off and fast positive force tracking) are formulated as hybrid optimal control problems. A formal analysis as well as experimental results demonstrate the properties and the quality of the control laws.
A Combined Series-Parallel Hybrid Envelope Amplifier for Envelope Tracking Mobile Terminal RF Power Amplifier Applications (2012)

An improved envelope amplifier architecture for envelope tracking RF power amplifiers is presented, consisting of two switching amplifiers and one linear amplifier. The first switching amplifier and the linear amplifier provide wideband and high-efficiency operation, while the second switching amplifier provides a reduced bandwidth variable supply to the linear amplifier to further reduce power loss. The first switching amplifier and the linear amplifier are fabricated together in a 150 nm CMOS process, while the second switching amplifier is external. Measurements show a maximum average efficiency of 82% for a 10 MHz LTE signal with a 6 dB PAPR at 29.7 dBm output power and an SFDR of 63 dBc for a single tone of 5 MHz driving an 8 Ω load.
TRAIL/Apo2L ligand selectively induces apoptosis and overcomes drug resistance in multiple myeloma: therapeutic applications (2001)

Multiple myeloma (MM) remains incurable and novel treatments are urgently needed. Preclinical in vitro and in vivo evaluations were performed to assess the potential therapeutic applications of human recombinant tumor necrosis factor (TNF)-related apoptosis-inducing ligand/Apo2 ligand (TRAIL/Apo2L) in MM. TRAIL/Apo2L potently induced apoptosis of MM cells from patients and the majority of MM cell lines, including cells sensitive or resistant to dexamethasone (Dex), doxorubicin (Dox), melphalan, and mitoxantrone. TRAIL/Apo2L also overcame the survival effect of interleukin 6 on MM cells and did not affect the survival of peripheral blood and bone marrow mononuclear cells and purified B cells from healthy donors. The status of the TRAIL receptors (assessed by immunoblotting and flow cytometry) could not predict TRAIL sensitivity of MM cells. The anti-MM activity of TRAIL/Apo2L was confirmed in nu/xid/bg mice xenografted with human MM cells; TRAIL (500 microg intraperitoneally daily for 14 days) was well tolerated and significantly suppressed the growth of plasmacytomas. Dox up-regulated the expression of the TRAIL receptor death receptor 5 (DR5) and synergistically enhanced the effect of TRAIL not only against MM cells sensitive to, but also against those resistant to, Dex- or Dox-induced apoptosis. Nuclear factor (NF)-kappaB inhibitors, such as SN50 (a cell-permeable inhibitor of the nuclear translocation and transcriptional activity of NF-kappaB) or the proteasome inhibitor PS-341, enhanced the proapoptotic activity of TRAIL/Apo2L against TRAIL-sensitive MM cells, whereas SN50 reversed the TRAIL resistance of ARH-77 and IM-9 MM cells. Importantly, normal B lymphocytes were not sensitized to TRAIL by either Dox, SN50, or PS-341. These preclinical studies suggest that TRAIL/Apo2L can overcome conventional drug resistance and provide the basis for clinical trials of TRAIL-based treatment regimens to improve outcome in patients with MM. (Blood. 2001;98:795-804)
Molecular Cloning of the Endogenous Rat C-type Helper Virus DNA Sequence: Structural Organization and Functional Analysis of Some Restricted DNA Fragments (1982)

Recently, we have identified and purified the integrated and proviral DNA sequences specific for two endogenous rat type C leukaemia helper viruses: WR-RaLV which originated from a fibrosarcoma induced in a feral rat and RHHV from the cell line HTC-H1 which originated from a Buffalo rat hepatoma. The rat leukaemia helper virus DNA sequences have previously been shown to be 8.4 to 8.8 kilobases (kb) in size. In this communication, we report the molecular cloning of the 8.8 kb DNA of RHHV by ligation at the BamHI site of the vector pBR322, cultured in an Escherichia coli RR1 host. After screening 5750 clones for ampicillin resistance and tetracycline sensitivity and testing by colony hybridization using 32P-labelled RHHV cDNA, four clones were isolated, two of which carried the total 8.8 kb DNA. A detailed restriction endonuclease map of the cloned RHHV DNA was deduced by sequential digestions of either 3'- or 5'-labelled DNA. Of the 14 restriction enzymes tested, EcoRI, BamHI, PstI, KpnI, TaqI, PvuII and SmaI gave informative cleavage patterns. At least two copies of long terminal repeated sequences (LTR) flanking the 3' and 5' termini of the proviral DNA were identified by TaqI and PstI cleavages. LTR in the rat endogenous leukaemia helper virus DNA measured 780 +/- 20 nucleotides in length. The genetic information encoded by the cloned DNA was also analysed by hybridization selection of RHHV mRNA, which was then used in cell-free protein synthesis in a rabbit reticulocyte lysate system. Essentially all major RaLV-specific proteins precipitable by anti-RaLV serum were synthesized in vitro, confirming that the RHHV genomic DNA was successfully cloned with little fidelity loss or scrambling of the genetic information.
A proposed `metabolism' for a hydrocarbon assembler (1997)

Molecular manufacturing should let us synthesize most arrangements of atoms that are consistent with physical law. Assemblers have been proposed as a means of accomplishing this objective. They would be able to build a wide range of useful products as well as copies of themselves. A simpler though less general proposal is a hydrocarbon assembler, restricted to manufacturing relatively stiff hydrocarbons. The design and analysis of such an assembler should be substantially simpler than that of a more general assembler. In this paper, we consider the `intermediary metabolism' of a hydrocarbon assembler, i.e. the set of reactions that permit processing of the feedstock molecules and their conversion into molecular tools (positionally controlled carbenes, radicals, and other reactive species). The specific feedstock molecule analyzed is butadiyne (a linear molecule, , also known as diacetylene; not to be confused with the more common but chemically distinct nonlinear molecule butadiene: ).
The Design And Clinical Use Of A Reflective Brachial Photoplethysmograph (2002)

This report concerns the design and clinical use of a reflective brachial photoplethysmograph. A plethysmograph is an instrument to obtain tracings showing volume changes of a part of the body. Originally this related to volume variations due to blood circulation within the body part of interest. The instrument is said to have been invented by Mosso of Turin around 1870 [1], known in Italian as a "pletismografo", and first reported in Scientific American in July 1872. A photoplethysmograph is an optical detector that indicates the volume of blood in or passing through an area of tissue. By placing the photoplethysmograph at or near the site of a human artery the pulse waveform can be detected and measured. The photoplethysmograph can be transmissive or reflective. There are a variety of sites around the body that are commonly used for detecting the pulse waveform including the finger, the ear lobe, and the foot. The device developed in this work is a reflective detector that uses the brachial artery as a photoplethysmographic site. There appear to be no prior indications in academic or patent literature of this site being used with this type of detector and consequently the authors believe this device to be novel and worthy of reporting to the research community. &#13;\n
Single isolated droplets with net charge as a source of ions (2000)

A levitation device for charged particles has been used to position a single isolated droplet at a time at atmospheric pressure near the sampling orifice of a vacuum chamber. Following a brief desolvation period (550 ms), a series of coluomb fission events were initiated. Several atmospheric pressure electrode designs were evaluated with respect to guiding the progeny droplets/ions, to the sampling orifice. The best design tested consisted of a series of four annular electrodes of decreasing radius positioned above the levitation ring electrodes, and, on average, 40 ions were counted per single isolated droplet. The ion utilization (charge detected versus charge in the original droplet) with this electrode design has been estimated to be 5 x 10(-6), a substantial improvement over the current utilization measured to be < or = 1 x 10(-9) with a conventional electrospray ion source using the same vacuum apparatus.
Roles of the Ig κ Light Chain Intronic and 3′ Enhancers in Igk Somatic Hypermutation (2006)

Somatic hypermutation (SHM) of the rearranged Ig genes is required for the affinity maturation of Abs. SHM is almost exclusively targeted to the rearranged Ig loci, but the mechanism of this gene-specific targeting remains unclear. The Ig kappa L chain locus contains multiple enhancers, including the MAR/intronic (iE(kappa)) and 3' enhancers (3'E(kappa)). Previous transgenic studies indicate that both kappa enhancers are individually necessary for SHM of Igk. In contrast, later studies of Ag-selected V(kappa) genes in 3'E(kappa)(-/-) mice found no absolute requirement for 3'E(kappa) in kappa SHM. To address the roles of the two kappa enhancers in SHM in a physiological context, we analyzed SHM of the endogenous Igk in mice with a targeted deletion of either iE(kappa) or 3'E(kappa) in Peyer's patch germinal center B cells. Our findings indicate that, although 3'E(kappa) is quantitatively important for SHM of Igk, iE(kappa) is not required for kappa SHM. In addition, a reduction of kappa mRNA levels is also detected in activated 3'E(kappa)(-/-) B cells. These findings suggest that iE(kappa) and 3'E(kappa) play distinct roles in regulating Igk transcription and SHM.
Fetal origins of insulin resistance and obesity (2005)

A number of epidemiological studies worldwide have demonstrated a relationship between poor early growth and an increased susceptibility to insulin resistance, visceral obesity, type 2 diabetes and other features of the metabolic syndrome in adulthood. However, the mechanistic basis of this relationship and the relative roles of genes and the environment remain a subject of debate. The 'thrifty phenotype' hypothesis proposes that poor fetal nutrition leads to programming of metabolism and an adult phenotype that is adapted to poor but not plentiful nutrition. The maternal reduced-protein rat model has been used to examine the importance of the maternal environment in determining susceptibility to adult disease. Pregnant and lactating rat dams are fed a diet containing 80 g protein/kg as compared with 200 g protein/kg, which leads to growth restriction in utero. Offspring of low-protein dams have increased susceptibility to diabetes, insulin resistance and hypertension when fed a palatable high-fat diet that promotes obesity. Administration of leptin during pregnancy and lactation to these protein-restricted dams produces offspring that have increased metabolic rate and do not become obese or insulin resistant when fed on a high-fat diet. Increased glucocorticoid exposure, particularly during late gestation, has been linked with insulin resistance in adulthood. High levels of fetal glucocorticoids may result from a decreased activity of placental 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 2, which normally protects the fetus from high maternal glucocorticoid levels. Leptin administration to protein-restricted dams inhibits the suppression of 11beta-HSD-2 and may be one mechanism by which the metabolic syndrome is prevented.
High-precision figure correction of x-ray telescope optics using ion implantation (2013)

Achieving both high resolution and large collection area in the next generation of x-ray telescopes requires highly accurate shaping of thin mirrors, which is not achievable with current technology. Ion implantation offers a promising method of modifying the shape of mirrors by imparting internal stresses in a substrate, which are a function of the ion species and dose. This technique has the potential for highly deterministic substrate shape correction using a rapid, low cost process. Wafers of silicon and glass (D-263 and BK-7) have been implanted with Si+ ions at 150 keV, and the changes in shape have been measured using a Shack-Hartmann metrology system. We show that a uniform dose over the surface repeatably changes the spherical curvature of the substrates, and we show correction of spherical curvature in wafers. Modeling based on experiments with spherical curvature correction shows that ion implantation could be used to eliminate higher-order shape errors, such as astigmatism and coma, by using a spatially-varying implant dose. We will report on progress in modelling and experimental tests to eliminate higher-order shape errors. In addition, the results of experiments to determine the thermal and temporal stability of implanted substrates will be reported.