gebhart/patrepeval huggingface dataset:
Aberrant Recruitment of the Nuclear Receptor Corepressor-Histone Deacetylase Complex by the Acute Myeloid Leukemia Fusion Partner ETO (1998)

Nuclear receptor corepressor (CoR)-histone deacetylase (HDAC) complex recruitment is indispensable for the biological activities of the retinoic acid receptor fusion proteins of acute promyelocytic leukemias. We report here that ETO (eight-twenty-one or MTG8), which is fused to the acute myelogenous leukemia 1 (AML1) transcription factor in t(8;21) AML, interacts via its zinc finger region with a conserved domain of the corepressors N-CoR and SMRT and recruits HDAC in vivo. The fusion protein AML1-ETO retains the ability of ETO to form stable complexes with N-CoR/SMRT and HDAC. Deletion of the ETO C terminus abolishes CoR binding and HDAC recruitment and severely impairs the ability of AML1-ETO to inhibit differentiation of hematopoietic precursors. These data indicate that formation of a stable complex with CoR-HDAC is crucial to the activation of the leukemogenic potential of AML1 by ETO and suggest that aberrant recruitment of corepressor complexes is a general mechanism of leukemogenesis.
Surface expression and functional characterization of α-granule factor V in human platelets: effects of ionophore A23187, thrombin, collagen, and convulxin (2000)

Factor V (FV) present in platelet alpha-granules has a significant but incompletely understood role in hemostasis. This report demonstrates that a fraction of platelets express very high levels of surface-bound, alpha-granule FV on simultaneous activation with 2 agonists, thrombin and convulxin, an activator of the collagen receptor glycoprotein VI. This subpopulation of activated platelets represents 30.7% +/- 4.7% of the total population and is referred to as convulxin and thrombin-induced-FV (COAT-FV) platelets. COAT-FV platelets are also observed on activation with thrombin plus collagen types I, V, or VI, but not with type III. No single agonist examined was able to produce COAT-FV platelets, although ionophore A23187 in conjunction with either thrombin or convulxin did generate this population. COAT-FV platelets bound annexin-V, indicating exposure of aminophospholipids and were enriched in young platelets as identified by the binding of thiazole orange. The functional significance of COAT-FV platelets was investigated by demonstrating that factor Xa preferentially bound to COAT-FV platelets, that COAT-FV platelets had more FV activity than either thrombin or A23187-activated platelets, and that COAT-FV platelets were capable of generating more prothrombinase activity than any other physiologic agonist examined. Microparticle production by dual stimulation with thrombin and convulxin was less than that observed with A23187, indicating that microparticles were not responsible for all the activities observed. These data demonstrate a new procoagulant component produced from dual stimulation of platelets with thrombin and collagen. COAT-FV platelets may explain the unique role of alpha-granule FV and the hemostatic effectiveness of young platelets. (Blood. 2000;95:1694-1702)
Monoclonal Antibodies (2020)

Introduction, H. Zola Making and using 'conventional' mouse monoclonal antibodies, P.J. Donohoe et al Human monoclonal antibodies from immortalized B cells, A.W. Hohmann et al Reconstruction of monoclonal antibodies by genetic engineering, J. Adair and
Enhancing electrochemical intermediate solvation through electrolyte anion selection to increase nonaqueous Li–O 2 battery capacity (2015)

Among the "beyond Li-ion" battery chemistries, nonaqueous Li-O2 batteries have the highest theoretical specific energy and, as a result, have attracted significant research attention over the past decade. A critical scientific challenge facing nonaqueous Li-O2 batteries is the electronically insulating nature of the primary discharge product, lithium peroxide, which passivates the battery cathode as it is formed, leading to low ultimate cell capacities. Recently, strategies to enhance solubility to circumvent this issue have been reported, but rely upon electrolyte formulations that further decrease the overall electrochemical stability of the system, thereby deleteriously affecting battery rechargeability. In this study, we report that a significant enhancement (greater than fourfold) in Li-O2 cell capacity is possible by appropriately selecting the salt anion in the electrolyte solution. Using (7)Li NMR and modeling, we confirm that this improvement is a result of enhanced Li(+) stability in solution, which, in turn, induces solubility of the intermediate to Li2O2 formation. Using this strategy, the challenging task of identifying an electrolyte solvent that possesses the anticorrelated properties of high intermediate solubility and solvent stability is alleviated, potentially providing a pathway to develop an electrolyte that affords both high capacity and rechargeability. We believe the model and strategy presented here will be generally useful to enhance Coulombic efficiency in many electrochemical systems (e.g., Li-S batteries) where improving intermediate stability in solution could induce desired mechanisms of product formation.
veA Is Required for Toxin and Sclerotial Production in Aspergillus parasiticus (2004)

It was long been noted that secondary metabolism is associated with fungal development. In Aspergillus nidulans, conidiation and mycotoxin production are linked by a G protein signaling pathway. Also in A. nidulans, cleistothecial development and mycotoxin production are controlled by a gene called veA. Here we report the characterization of a veA ortholog in the aflatoxin-producing fungus A. parasiticus. Cleistothecia are not produced by Aspergillus parasiticus; instead, this fungus produces spherical structures called sclerotia that allow for survival under adverse conditions. Deletion of veA from A. parasiticus resulted in the blockage of sclerotial formation as well as a blockage in the production of aflatoxin intermediates. Our results indicate that A. parasiticus veA is required for the expression of aflR and aflJ, which regulate the activation of the aflatoxin gene cluster. In addition to these findings, we observed that deletion of veA reduced conidiation both on the culture medium and on peanut seed. The fact that veA is necessary for conidiation, production of resistant structures, and aflatoxin biosynthesis makes veA a good candidate gene to control aflatoxin biosynthesis or fungal development and in this way to greatly decrease its devastating impact on health and the economy.
The dual-bootstrap iterative closest point algorithm with application to retinal image registration (2003)

Motivated by the problem of retinal image registration, this paper introduces and analyzes a new registration algorithm called Dual-Bootstrap Iterative Closest Point (Dual-Bootstrap ICP). The approach is to start from one or more initial, low-order estimates that are only accurate in small image regions, called bootstrap regions. In each bootstrap region, the algorithm iteratively: 1) refines the transformation estimate using constraints only from within the bootstrap region; 2) expands the bootstrap region; and 3) tests to see if a higher order transformation model can be used, stopping when the region expands to cover the overlap between images. Steps 1): and 3), the bootstrap steps, are governed by the covariance matrix of the estimated transformation. Estimation refinement [Step 2)] uses a novel robust version of the ICP algorithm. In registering retinal image pairs, Dual-Bootstrap ICP is initialized by automatically matching individual vascular landmarks, and it aligns images based on detected blood vessel centerlines. The resulting quadratic transformations are accurate to less than a pixel. On tests involving approximately 6000 image pairs, it successfully registered 99.5% of the pairs containing at least one common landmark, and 100% of the pairs containing at least one common landmark and at least 35% image overlap.
Queer Current and Pacemaker: The Hyperpolarization-Activated Cation Current in Neurons (1996)

The cation conductance activated upon hyperpolarization of the membrane beyond the resting value appears to represent an ubiquitous type of membrane channel. Our understanding of the respective membrane current, termed Ih, in neurons has matured from that of a "queer" current toward that of a highly regulated mechanism that is particularly important in determining integrative behavior near rest and providing the pacemaker depolarization during rhythmic-oscillatory activity.
Accurate Calculation of Hydration Free Energies Using Macroscopic Solvent Models (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAccurate Calculation of Hydration Free Energies Using Macroscopic Solvent ModelsDoree Sitkoff, Kim A. Sharp, and Barry HonigCite this: J. Phys. Chem. 1994, 98, 7, 1978–1988Publication Date (Print):February 1, 1994Publication History Published online1 May 2002Published inissue 1 February 1994https://pubs.acs.org/doi/10.1021/j100058a043https://doi.org/10.1021/j100058a043research-articleACS PublicationsRequest reuse permissionsArticle Views4823Altmetric-Citations1836LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Elevated paraquat resistance can be used as a bioassay for longevity in a genetically based long‐lived strain of Drosophila (1991)

A long-lived (L) strain of Drosophila melanogaster, derived from a normal-lived (R) strain by artificial selection, has a significantly different adult longevity. Previous work has shown that 1) the two strains age in the same manner, 2) the major genes responsible for much of the L strain's extended longevity are located on the 3rd chromosome, and 3) the extended longevity phenotype is significantly modulated by the larval environment. In this report, we investigate the resistance of the L and R strains to the lethal effects of dietary paraquat. We show that, within the limitations of our described chromosomal and environmental manipulations, the extended longevity phenotype always accompanies the phenotype of elevated paraquat resistance. In addition, reversed selection applied to the L strain results in the simultaneous decrease of both life span and paraquat resistance. Thus, the presence or absence of the latter phenotype may be used as a bioassay for the presence or absence of the extended longevity phenotype, without any necessary implication of causality. Use of this bioassay should greatly speed up the genetic analysis of this system by allowing us to identify long-lived animals at a young age. Finally, we show that the age-related loss of elevated paraquat resistance in both strains precedes all the other age-related functional decrements which we have previously noted in this system.
Discovery of new markers of cancer through serial analysis of gene expression: prostate stem cell antigen is overexpressed in pancreatic adenocarcinoma. (2001)

Serial analysis of gene expression (SAGE) can be used to quantify gene expression in human tissues. Comparison of gene expression levels in neoplastic tissues with those seen in nonneoplastic tissues can, in turn, identify novel tumor markers. Such markers are urgently needed for highly lethal cancers like pancreatic adenocarcinoma, which typically presents at an incurable, advanced stage. The results of SAGE analyses of a large number of neoplastic and nonneoplastic tissues are now available online, facilitating the rapid identification of novel tumor markers. We searched an online SAGE database to identify genes preferentially expressed in pancreatic cancers as compared with normal tissues. SAGE libraries derived from pancreatic adenocarcinomas were compared with SAGE libraries derived from nonneoplastic tissues. Three promising tags were identified. Two of these tags corresponded to genes (lipocalin and trefoil factor 2) previously shown to be overexpressed in pancreatic carcinoma, whereas the third tag corresponded to prostate stem cell antigen (PSCA), a recently discovered gene thought to be largely restricted to prostatic basal cells and prostatic adenocarcinomas. PSCA was expressed in four of the six pancreatic cancer SAGE libraries, but not in the libraries derived from normal pancreatic ductal cells. We confirmed the overexpression of the PSCA mRNA transcript in 14 of 19 pancreatic cancer cell lines by reverse transcription-PCR, and using immunohistochemistry, we demonstrated PSCA protein overexpression in 36 of 60 (60%) primary pancreatic adenocarcinomas. In 59 of 60 cases, the adjacent nonneoplastic pancreas did not label for PSCA. PSCA is a novel tumor marker for pancreatic carcinoma that has potential diagnostic and therapeutic implications. These results establish the validity of analyses of SAGE databases to identify novel tumor markers.
Metrics for the evaluation of news site content layout in large-screen contexts (2011)

Despite the fact that screen sizes and average screen resolutions have dramatically increased over the past few years, little attention has been paid to the design of web sites for large, high-resolution displays that are now becoming increasingly used both in enterprise and consumer spaces. We present a study of how the visual area of the browser window is currently utilised by news web sites at different widescreen resolutions. The analysis includes measurements of space taken up by the article content, embedded ads and the remaining components as they appear in the viewport of the web browser. The results show that the spatial distribution of page elements does not scale well with larger viewing sizes, which leads to an increasing amount of unused screen real estate and unnecessary scrolling. We derive a number of device-sensitive metrics to measure the quality of web page layout in different viewing contexts, which can guide the design of flexible layout templates that scale effectively on large screens.
Correction of Down syndrome and Edwards syndrome aneuploidies in human cell cultures (2015)

Aneuploidy, an abnormal number of chromosomes, has previously been considered irremediable. Here, we report findings that euploid cells increased among cultured aneuploid cells after exposure to the protein ZSCAN4, encoded by a mammalian-specific gene that is ordinarily expressed in preimplantation embryos and occasionally in stem cells. For footprint-free delivery of ZSCAN4 to cells, we developed ZSCAN4 synthetic mRNAs and Sendai virus vectors that encode human ZSCAN4. Applying the ZSCAN4 biologics to established cultures of mouse embryonic stem cells, most of which had become aneuploid and polyploid, dramatically increased the number of euploid cells within a few days. We then tested the biologics on non-immortalized primary human fibroblast cells derived from four individuals with Down syndrome—the most frequent autosomal trisomy of chromosome 21. Within weeks after ZSCAN4 application to the cells in culture, fluorescent in situ hybridization with a chromosome 21-specific probe detected the emergence of up to 24% of cells with only two rather than three copies. High-resolution G-banded chromosomes further showed up to 40% of cells with a normal karyotype. These findings were confirmed by whole-exome sequencing. Similar results were obtained for cells with the trisomy 18 of Edwards syndrome. Thus a direct, efficient correction of aneuploidy in human fibroblast cells seems possible in vitro using human ZSCAN4.
Quantum effects in electrical and thermal transport through nanowires (2001)

Nanowires, point contacts and metallic single-wall carbon nanotubes are onedimensional nanostructures which display important size-dependent quantum effects. Quantization due to the transverse confinement and resultant finite level spacing of electronic and phononic states are responsible for some novel effects. Many studies have revealed fundamental and technologically important properties, which are being explored for fabricating future nanodevices. Various simulation studies based on the classical molecular dynamics method and combined force and current measurements have shown the relationship between atomic structure and transport properties. The atomic, electronic and transport properties of these nanostructures have been an area of active research. This brief review presents some quantum effects in the electronic and phononic transport through nanowires.
Collagen-binding growth factors: Production and characterization of functional fusion proteins having a collagen-binding domain (1998)

The autocrine/paracrine peptide signaling molecules such as growth factors have many promising biologic activities for clinical applications. However, one cannot expect specific therapeutic effects of the factors administered by ordinary drug delivery systems as they have limited target specificity and short half-lives in vivo. To overcome the difficulties in using growth factors as therapeutic agents, we have produced fusion proteins consisting of growth factor moieties and a collagen-binding domain (CBD) derived from Clostridium histolyticum collagenase. The fusion proteins carrying the epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) at the N terminal of CBD (CBEGF/CBFGF) tightly bound to insoluble collagen and stimulated the growth of BALB/c 3T3 fibroblasts as much as the unfused counterparts. CBEGF, when injected subcutaneously into nude mice, remained at the sites of injection for up to 10 days, whereas EGF was not detectable 24 h after injection. Although CBEGF did not exert a growth-promoting effect in vivo, CBFGF, but not bFGF, strongly stimulated the DNA synthesis in stromal cells at 5 days and 7 days after injection. These results indicate that CBD may be used as an anchoring unit to produce fusion proteins nondiffusible and long-lasting in vivo.
Control of concentration and volume gradients in microfluidic droplet arrays for protein crystallization screening (2005)

A novel screening platform for the screening and optimization of protein crystallization is reported. Here we present two key experiments to generate protein crystallization phase diagrams. One is to precisely generate spatial gradient droplet arrays; the other is to trap and identify each droplet in place in order to carry out temporal and spatial analysis. The generated concentration and volume gradient ranges from 0% to 100% and from 155 to 310 pico liters, respectively. Furthermore, the active trapping of a droplet array in a microfluidic chip for hours to days to study the dynamic phase changes in protein crystallization is demonstrated. This microfluidic platform can be used for rapidly generating the solubility diagram for protein in various salt solutions.
Synthesis and Characterization of Zirconogermanates (2003)

Six new zirconogermanates have been prepared under hydrothermal conditions using amines as bases. There are four new structure types (ASU-n) with a common motif of ZrGe(5). ASU-23 is a layered structure: ZrGe(3)O(8)(OH)F.[C(10)H(26)N(4)].H(2)O, space group P2(1)/n, a = 6.7957(8) A, b = 12.700(1) A, c = 24.293(3) A, beta = 97.936(2) degrees, V = 2076.4(4) A(3). ASU-24 is a pillared layered structure: Zr(3)Ge(6)O(18)(OH(2),F)(4)F(2).[C(6)H(18)N(2)].[C(6)H(17)N(2)](2).2H(2)O, space group P2(1)/n, a = 7.4249(3) A, b = 25.198(1) A, c = 11.3483(5) A, beta = 90.995(1) degrees, V = 2122.9(2) A(3). This material has the lowest framework density (FD) of any oxide material that we are aware of (FD = 8.48 metal atoms/nm(3)). Two other materials form three-dimensional open-frameworks, ASU-25: ZrGe(3)O(9).[C(3)H(12)N(2)], space group P112(1)/a, a = 13.1994(4) A, b = 7.6828(2) A, c = 11.2373(3) A, gamma = 91.233(3) degrees, V = 1139.29(5) A(3). The other is ASU-26: ZrGe(3)O(9).[C(2)H(10)N(2)], space group Pn, a = 13.7611(3) A, b = 7.7294(2) A, c = 11.2331(3) A, beta = 104.793(1) degrees, V = 1155.21(4) A(3). ASU-25 is related to the mineral umbite K(2)ZrSi(3)O(9).H(2)O. The germanium equivalent has been prepared through the inorganic route: K(2)ZrGe(3)O(9).H(2)O, space group P2(1)2(1)2(1), a = 13.6432(6) A, b = 7.4256(3) A, c = 10.3973(4) A, V = 1053.33(8) A(3). The structural relationships between ASU-25 and its inorganic counterpart are described. The thermal decomposition of the germanium umbite generated the cyclic trigermanate K(2)ZrGe(3)O(9), analogue of the mineral wadeite, crystallizing in the orthorhombic system, a = 7.076 A, b = 12.123 A, c = 10.451 A, V = 904.5 A(3).
Stickstoffhaltige Steroide, XIV. Darstellung und Ringöffnungsreaktionen der epimeren 2.3‐Imino‐cholestane (1966)

Abstract 2α.3α‐ und 2β.3β‐Imino‐cholestan werden durch Reduktion der entsprechenden vicinalen Azidoalkohol‐methansulfonsäureester mit Lithiumaluminiumhydrid dargestellt. Die Struktur der Aziridine wird durch Desaminierung mit salpetriger Säure zu Δ 2 ‐Cholesten gesichert. Einige Derivate der Aziridine werden gewonnen und Ringöffnungsreaktionen der Aziridine und ihrer Derivate untersucht.
Growth promoting activity of IGF-I in the rat. (1988)

According to the original somatomedin hypothesis, GH promotes growth by generating 'somatomedins' or insulin-like growth factors (IGFs) in the liver. The advent of large amounts of IGF-I produced by recombinant DNA technology has now allowed testing of this hypothesis, by comparing the growth promoting activity of IGF-I and GH in three animal models of growth deficiency. When injected or infused subcutaneously, or infused intravenously, IGF-I is a weak growth promoter in the hypophysectomized rat compared with GH, even when infused in combination with small amounts of GH. Growth arrest in the diabetic rat was corrected by insulin infusion which also restored GH secretion. Insulin or IGF-I caused a large initial weight gain in diabetic rats, accompanied by a partial correction of food and water balance, even in the presence of persistent hyperglycaemia. A new mutant GH deficient dwarf rat grows in response to both GH and IGF-I infusions, but these agents elicit different patterns of organ growth. For the same overall body growth, GH was more effective in stimulating bone growth, whereas IGF-I stimulated renal and splenic growth. This new dwarf rat may prove useful for the study of the relative growth promoting effects of IGF-I and GH in more detail in future.
[Long-term results of interferential current stimulation in the treatment of bladder instability]. (2001)

Treatment of detrusor instability by interferential current is a reliable technique which constitutes an alternative to the other methods of retraining stimulation and can be performed in cases of instability refractory to anticholinergic drugs, before considering neuromodulation or surgery. Five to 10 maintenance sessions every 12 or 18 months ensure stable long-term results in the majority of cases.