gebhart/patrepeval huggingface dataset:
Galactosyltransferases: A Structural Overview of Their Function and Reaction Mechanisms. (2001)

ガラクトース転移酵素は糖転移酵素のファミリーの中で最も代表的なものである。ガラクトース転移酵素はUDP-α-D-ガラクトースを供与体としてガラクトースを糖タンパク質、グリコアミノグリカン、糖脂質、あるいは植物ホルモンなどのような脂溶性分子などさまざまなアクセプターに転移する。過去10年間ほ乳類細胞のガラクトース転移酵素は糖質研究者の興味の中心であり遺伝子の発見と酵素反応機構の解明に焦点がおかれてきた。1999年にウシβ4GalT1の触媒ドメインの結晶構造解析の結果が分解能2.4Åで初めて明らかになった。この研究は、原子レベルでの立体構造に着目することにより酵素的性質を解釈し反応機構を理解することをめざす、糖転移酵素の構造的研究の新展開の幕開けとなった。この総説では糖転移酵素のファミリーの流れの中でガラクトース転移酵素の構造ー機能相関の現状をとりあげる。2つのウシガラクトース転移酵素、β4GalT1, α3GalT、の触媒ドメインの結晶構造と反応機構を考察する。
Optical pulse propagation in metal nanoparticle chain waveguides (2003)

Finite-difference time-domain simulations show direct evidence of optical pulse propagation below the diffraction limit of light along linear arrays of spherical noble metal nanoparticles with group velocities up to $0.06c.$ The calculated dispersion relation and group velocities correlate remarkably well with predictions from a simple point-dipole model. A change in particle shape to spheroidal particles shows up to a threefold increase in group velocity. Pulses with transverse polarization are shown to propagate with negative phase velocities antiparallel to the energy flow.
Synthesis and Coordination Properties of Nitrogen(I)‐Based Ligands (2010)

Give me four: In contrast to typical imines, the central nitrogen atom in cyclopropenyl-derived carbeneimines (see example; C gray, N blue) can donate up to four electrons to form coordination complexes.
Corticosteroid Regulation of P-Glycoprotein in the Developing Blood-Brain Barrier (2011)

The early fetal brain is susceptible to teratogens in the maternal circulation, because brain microvessel expression of drug efflux transporter, P-glycoprotein (P-gp), is very low. However, there is a dramatic up-regulation of brain microvessel P-gp in late gestation. This study investigated the role of cortisol and dexamethasone in this up-regulation of fetal brain microvessel P-gp expression. Primary brain endothelial cell (BEC) cultures derived from gestational d (GD)40, GD50, GD65 (term, ∼68 d) and postnatal d 14 male guinea pigs were treated with varying doses (10(-8) to 10(-5) m) of cortisol, dexamethasone, and aldosterone. After treatment, P-gp function was assessed using calcein-acetoxymethyl ester (P-gp substrate; 1 μm for 1 h) and measuring BEC accumulation of calcein. Corticosteroid treatment of BECs derived from postnatal d 14 resulted in increased P-gp activity. BECs derived from GD65 (near term) responded similarly, but these cells were extremely sensitive to the effects of mineralocorticoid receptor agonists (cortisol and aldosterone). BECs derived from GD50 displayed dose-dependent increases in P-gp function with dexamethasone (P < 0.05) and a trend towards increased function with cortisol. Cells derived from GD40 were unresponsive to all treatments. In conclusion, P-gp function in BECs is more responsive to glucocorticoids (GCs) in late gestation. Therefore, the late gestational surge in fetal plasma GCs, which parallels the increase in brain microvessel P-gp expression, may contribute to this P-gp up-regulation. Further, synthetic GCs (administered to pregnant women at risk of preterm delivery) may increase the protective capacity of the developing fetal blood-brain barrier, depending on the timing of GC exposure.
Plasma mSEPT9: A Novel Circulating Cell-free DNA-Based Epigenetic Biomarker to Diagnose Hepatocellular Carcinoma (2018)

Among patients with cirrhosis, the mSEPT9 test constitutes a promising circulating epigenetic biomarker for HCC diagnosis at the individual patient level. Future prospective studies should assess the mSEPT9 test in the screening algorithm for cirrhotic patients to improve risk prediction and personalized therapeutic management of HCC.
Cell Cycle Arrest and Apoptosis Induced by Human Polo-Like Kinase 3 Is Mediated through Perturbation of Microtubule Integrity (2002)

Human Polo-like kinase 3 (Plk3, previously termed Prk or Fnk) is involved in regulation of cell cycle progression through the M phase (B. Ouyang, H. Pan, L. Lu, J. Li, P. Stambrook, B. Li, and W. Dai, J. Biol. Chem. 272:28646-28651, 1997). Here we report that in most interphase cells endogenous Plk3 was predominantly localized around the nuclear membrane. Double labeling with Plk3 and gamma-tubulin, the latter a major component of pericentriole materials, revealed that Plk3 was closely associated with centrosomes and that its localization to centrosomes was dependent on the integrity of microtubules. Throughout mitosis, Plk3 appeared to be localized to mitotic apparatus such as spindle poles and mitotic spindles. During telophase, a significant amount of Plk3 was also detected in the midbody. Ectopic expression of Plk3 mutants dramatically changed cell morphology primarily due to their effects on microtubule dynamics. Expression of a constitutively active Plk3 (Plk3-A) resulted in rapid cell shrinkage, which led to formation of cells with an elongated, unsevered, and taxol-sensitive midbody. In contrast, cells expressing a kinase-defective Plk3 (Plk3(K52R)) mutant exhibited extended, deformed cytoplasmic structures, the phenotype of which was somewhat refractory to taxol treatment. Expression of both Plk3-A and Plk3(K52R) induced apparent G(2)/M arrest followed by apoptosis, although the kinase-defective mutant was less effective. Taken together, our studies strongly suggest that Plk3 plays an important role in the regulation of microtubule dynamics and centrosomal function in the cell and that deregulated expression of Plk3 results in cell cycle arrest and apoptosis.
Dimethyl phosphate hydrolysis at neutral pH (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDimethyl phosphate hydrolysis at neutral pHJung Hee Kim and Jik ChinCite this: J. Am. Chem. Soc. 1992, 114, 25, 9792–9795Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December 1992https://pubs.acs.org/doi/10.1021/ja00051a011https://doi.org/10.1021/ja00051a011research-articleACS PublicationsRequest reuse permissionsArticle Views973Altmetric-Citations71LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Fast and Efficient Ion-Exchange Procedure To Remove Surfactant Molecules from MCM-41 Materials (2004)

A pure silica MCM-41 and two aluminosilicates with Si/Al = 1.25 and 30 have been synthesized hydrothermally under basic conditions in the presence of cationic surfactants. Organic molecules could be completely extracted from the mesopores by treating as-made compounds with an ethanolic solution of ammonium nitrate at 333 K. The procedure is particularly efficient for the low-silica compound: all surfactant molecules are removed within less than 15 min. After treatment, NH4+ cations, which balance the charge of aluminum atoms in aluminosilicates, can be removed by heating extracted Al-MCM-41 at moderate temperature. In the pure-silica material, the in-situ decomposition of NH4+ cations directly forms silanol groups on the surface of the mesopores. Extracted materials possess highly ordered structures with properties similar to those of directly calcined materials. The procedure reported here is fast, cheap, and efficient to remove completely surfactant molecules from the pores of MCM-41 materials with various compositions.
Call for papers (2002)

Prospective authors are requested to submit new, unpublished manuscripts for inclusion in the upcoming event described in this call for papers.
Collagen-based devices for soft tissue repair (1996)

The intrinsic biological and physiochemical characteristics of collagen have been exploited to prepare a variety of commercially available medical products spanning many medical specialties. The properties of purified collagen can be modified to obtain forms which comply to specific applications. In particular, collagen materials in the form of gels, matrices, and films have been applied, either alone or in combination with other agents, to address soft tissue repair. This review describes how the versatile properties of collagen have made it one of the most useful biomaterials for wound care applications.
Intraperitoneal Adoptive Immunotherapy of Ovarian Carcinoma with Tumor-Infiltrating Lymphocytes and Low-Dose Recombinant Interleukin-2 (1994)

A pilot study was conducted in patients who had advanced epithelial ovarian carcinoma, and who were refractory to platinum-based chemotherapy, to determine the feasibility and clinical effects of a schedule of intraperitoneal (IP) tumor-infiltrating lymphocytes (TIL) expanded in recombinant interleukin-2 (rIL-2), and low-dose rIL-2 IP. TIL were expanded from solid metastases or malignant effusions in serum-free AIM V medium supplemented with low concentrations (600 IU/ml) or rIL-2 using a four-step method of expansion that included a hollow fiber bioreactor (artificial capillary culture system). Patients received IP TIL suspended in dextrose 5% in sodium chloride 0.2% containing 0.1% human albumin and 6 x 10(5) IU rIL-2 on day 1, followed by 6 x 10(5) IU rIL-2/m2 body surface area, administered daily by bolus IP injection, on days 2-4, 8-11, and 15-18. In the absence of disease progression, two additional 4-day cycles of IP rIL-2 were administered. Patients (n = 3) whose TIL failed to grow in vitro received IP IL-2 alone. Eight patients received rIL-2 expanded TIL (10(10)-10(11) range) plus rIL-2 followed by several cycles of rIL-2 alone. One of these patients was treated twice with TIL plus rIL-2. Expanded TIL were primarily CD3+CD4+TCR alpha beta+ (eight TIL-derived T-cell lines). One TIL-derived T-cell line was comprised mostly of CD3+CD8+TCR alpha beta+ cells. Eleven patients (eight treated with TIL plus rIL-2 and three patients treated with rIL-2 alone) received a total of 38 cycles of rIL-2 without TIL. Grade 3 clinical toxicity (peritonitis) occurred in 1 of 9 cycles of TIL plus rIL-2 and 1 of 38 cycles of rIL-2 alone. Each cycle was 4 days long. Grade 3 anemia occurred in 1 of 9 TIL plus rIL-2 cycles and 3 of 38 cycles of rIL-2 alone. There were no measurable responses; however, four of eight patients treated with IP TIL plus rIL-2 had some indication of clinical activity: ascites regression (two patients), tumor and CA-125 reduction (one patient), and surgically confirmed stable tumor and CA-125 values (one patient). The schedule of IP TIL plus low-dose rIL-2 shows manageable toxicity and is worthy of further evaluation in patients with epithelial ovarian cancer who have less tumor burden.
Ibrutinib versus Ofatumumab in Previously Treated Chronic Lymphoid Leukemia (2014)

Ibrutinib, as compared with ofatumumab, significantly improved progression-free survival, overall survival, and response rate among patients with previously treated CLL or SLL. (Funded by Pharmacyclics and Janssen; RESONATE ClinicalTrials.gov number, NCT01578707.).
Photoacoustic Spectroscopy in Trace Gas Monitoring (2000)

Abstract Since ancient times people have searched for ways to understand processes occurring in the environment, atmosphere or living organisms. Study of the gaseous trace compounds present may shed new light on chemical reactions taking place in the atmosphere or biochemical reactions inside organisms such as plants, animals and human beings. This article presents photoacoustic spectroscopy as a sensitive, on‐line and noninvasive tool for monitoring the concentration of trace gases. Following a short introduction and a historic overview, attention is focused on the description of devices and equipment which determine the detection limits and selectivity. An overview is given of the current detection limits for photoacoustic detection. Applications are discussed with emphasis on environmental monitoring (in ambient air, car exhaust and stack gas emission), on medical applications and on biological applications (in postharvest physiology, plant physiology, microbiology and entomology).
PhoneyC: A Virtual Client Honeypot (2009)

The number of client-side attacks has grown significantly in the past few years, shifting focus away from defendable positions to a broad, poorly defended space filled with vulnerable clients. Just as honeypots enabled deep research into server-side attacks, honeyclients can permit the deep study of client-side attacks. A complement to honeypots, a honeyclient is a tool designed to mimic the behavior of a userdriven network client application, such as a web browser, and be exploited by an attacker’s content. These systems are instrumented to discover what happened and how. This paper presents PhoneyC, a honeyclient tool that can provide visibility into new and complex client-side attacks. PhoneyC is a virtual honeyclient, meaning it is not a real application but rather an emulated client. By using dynamic analysis, PhoneyC is able to remove the obfuscation from many malicious pages. Furthermore, PhoneyC emulates specific vulnerabilities to pinpoint the attack vector. PhoneyC is a modular framework that enables the study of malicious HTTP pages and understands modern vulnerabilities and attacker techniques. 1
Rituximab (anti-CD20 monoclonal antibody) for the treatment of patients with relapsing or refractory aggressive lymphoma: a multicenter phase II study. (1998)

Rituximab, a chimeric monoclonal antibody that binds specifically to the CD20 antigen, induced objective responses in 50% of patients with low-grade or follicular B-cell lymphoma. Because most nonfollicular B-cell lymphomas also express the CD20 antigen, we conducted a phase II study to evaluate the efficacy and tolerability of this new agent in patients with more aggressive types of lymphoma. Patients with diffuse large B-cell lymphoma (DLCL), mantle cell lymphoma (MCL), or other intermediate- or high-grade B-cell lymphomas according to the Working Formulation were included in this prospective randomized phase II study if they were in first or second relapse, if they were refractory to initial therapy, if they progressed after a partial response to initial therapy, or if they were elderly (age >60 years) and not previously treated. The patients received 8 weekly infusions of rituximab at the dose of 375 mg/m2 in arm A or one infusion of 375 mg/m2 followed by 7 weekly infusions of 500 mg/m2 in arm B. Patients were evaluated 2 months after the last rituximab infusion. Fifty-four patients were randomized from 9 centers in Europe and Australia (28 in arm A and 26 in arm B). A total of 5 complete responses (CR) and 12 partial responses (PR) were observed among the 54 enrolled patients, with no difference between the two doses. In an intent-to-treat analysis, the CR rate was 9% (CI95%, 3% to 20%) and the PR rate was 22% (CI95%, 12% to 36%), for an overall response rate of 31% (CI95%, 20% to 46%). An analysis of prognostic factors showed that response rates were lower in patients with refractory disease, patients with lymphoma not classified as DLCL, and patients with a tumor larger than 5 cm in diameter. DLCL and MCL patients had response rates of 37% and 33%, respectively. The median time to progression exceeded 246 days for the 17 responding patients. The most frequently reported adverse events were related to an infusion syndrome and were mild: 19% of the patients had a grade 3 related adverse event, slightly more in arm B, and only 1 patient had a grade 4 related adverse event in arm A. Two patients (3.7%) withdrew from treatment because of severe adverse events, one patient in each arm. In this first trial of rituximab in DLCL and MCL, patients experienced a significant clinical activity with a low toxicity. Rituximab has significant activity in DLCL and MCL patients and should be tested in combination with chemotherapy in such patients.
Concise Encyclopedia of Polymer Science and Engineering (1990)

A compact desk reference, this book includes all the subjects contained in the 17 alphabetical volumes and the Supplement and Index volumes of the Encyclopedia of Polymer Science and Engineering. The articles have been condensed by professional science writers, reviewed for accuracy by the original authors or their colleagues, and updated where necessary. Similar to the original set, this edition provides both SI and common units, carefully selected key references for each article, and hundreds of tables, charts, figures and graphs. This distillation, prepared to retain the factual material of the original, is a complete and self-contained encyclopaedia. It is designed to serve as a ready-reference guide for anyone seeking answers to questions on any aspect of polymer science and engineering.
Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation (1999)

Molecular genetic analysis of isolated single cells and other minute DNA samples is limited because there is insufficient DNA to perform more than one independent PCR amplification. One solution to this problem is to first amplify the entire genome, thus providing enough DNA for numerous subsequent PCRs. In this study we have investigated four different methods of whole genome amplification performed on single cells, and have identified a protocol that generates sufficient quantities of DNA for comparative genomic hybridisation (CGH) as well as more than 90 independent amplification reactions. Thus, numerous specific loci and the copy number of every chromosome can be assessed in a single cell. We report here the first reliable application of CGH to single cells from human preimplantation embryos (blastomeres) and to single fibroblasts, buccal cells and amniocytes.
Differential Endothelial Migration and Proliferation to Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor (1996)

Neovascularization is a feature of a variety of pathological processes. We compared the characteristics of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on migration and proliferation of human umbilical vein endothelium (HUVEC). Both VEGF and bFGF induced endothelial cell migration at similar concentrations (1/2 max. VEGF = approximately 1.0 ng/ml, bFGF = approximately 5.0 ng/ml). However, VEGF-stimulated migration was two-fold greater than bFGF at 1 and 10 ng/ml (p < 0.05). In contrast, bFGF induced proliferation four-fold more effectively than VEGF (1/2 max. 1 ng/ml and 1.4 ng/ml respectively). Checkerboard migration assays for bFGF showed a predominantly chemokinetic pattern, whereas VEGF was predominantly chemotactic. VEGF and bFGF were not synergistic in monolayer proliferation and migration assays. Three angiogenesis inhibitors, alpha-interferon, TNP-470, and platelet factor-4, inhibited VEGF and bFGF induced cell migration. These results indicate that VEGF and bFGF are chemoattractants that stimulate endothelial migration by different mechanisms and that both can be inhibited by known angiogenesis inhibitors.
Therapeutic interventions targeted at the augmentation of reverse cholesterol transport (2004)

Established therapies for raising HDL can be effective either as monotherapy or when used in combination. Newer strategies are being developed to exploit more specifically the capacity of HDL to drive RCT and either prevent or reverse the course of atherosclerotic disease.
iSAM: Incremental Smoothing and Mapping (2008)

In this paper, we present incremental smoothing and mapping (iSAM), which is a novel approach to the simultaneous localization and mapping problem that is based on fast incremental matrix factorization. iSAM provides an efficient and exact solution by updating a QR factorization of the naturally sparse smoothing information matrix, thereby recalculating only those matrix entries that actually change. iSAM is efficient even for robot trajectories with many loops as it avoids unnecessary fill-in in the factor matrix by periodic variable reordering. Also, to enable data association in real time, we provide efficient algorithms to access the estimation uncertainties of interest based on the factored information matrix. We systematically evaluate the different components of iSAM as well as the overall algorithm using various simulated and real-world datasets for both landmark and pose-only settings.
Molecular cloning of a protein associated with soybean seed oil bodies that is similar to thiol proteases of the papain family. (1990)

A 34,000-Da protein (P34) is one of the four major soybean oil body proteins observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of isolated organic solvent-extracted oil bodies from mature seeds. P34 is processed during seedling growth to a 32,000-Da polypeptide (P32) by the removal of an amino-terminal decapeptide (Herman, E.M., Melroy, D.L., and Buckhout, T.J. (1990) Plant Physiol, in press). A soybean lambda ZAP II cDNA library constructed from RNA isolated from midmaturation seeds was screened with monoclonal antibodies directed against two different epitopes of P34. The isolated cDNA clone encoding P34 contains 1,350 base pairs terminating in a poly(A)+ tail and an open reading frame 1,137 base pairs in length. The open reading frame includes a deduced amino acid sequence which matches 23 of 25 amino-terminal amino acids determined by automated Edman degradation of P34 and P32. The cDNA predicts a mature protein of 257 amino acids and of 28,641 Da. The open reading frame extends 5' from the known amino terminus of P34 encoding a possible precursor and signal sequence segments with a combined additional 122 amino acids. Prepro-P34 is deduced to be a polypeptide of 42,714 Da, indicating that the cDNA clone apparently encodes a polypeptide of 379 amino acids. A comparison of the nucleotide and deduced amino acid sequences in the GenBank Data Bank with the sequence of P34 has shown considerable sequence similarity to the thiol proteases of the papain family. Southern blot analysis of genomic DNA indicated that the P34 gene has a low copy number.
Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: Delineation by expression of CD27, IgD, and CD95 (2008)

We conclude that CD95 is a useful marker to identify CD27- memory B cells with an activated phenotype, which might serve as a biomarker for lupus activity and as a target of further investigations aiming to elucidate the pathogenic potential of these cells and the mechanisms involved in the generation as well as regulation of this CD27-,IgD-,CD95+ memory B cell subset.
THE RESPONSE OF OCULAR PRESSURE TO CORTICOSTEROIDS* (1966)

* Supported in part by a research grant, 5R01-NB-05224, from the National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland.
High‐Conductivity Elastomeric Electronics (2004)

Stretchable electronic circuits have been microfabricated using tortuous gold wires embedded in silicone elastomer. With appropriate geometric parameters, the wires could elongate by more than 50 % while maintaining stable conductivity (see Figure). This technology represents a key development towards conductive fabrics, flexible displays, and other applications that require electronics to withstand unprecedented degrees of stress and vibration.