gebhart/patrepeval huggingface dataset:
Combinatorial and High-Throughput Screening of the Effect of Siloxane Composition on the Surface Properties of Crosslinked Siloxane−Polyurethane Coatings (2006)

Libraries of siloxane-polyurethane coatings were designed, formulated, and screened using high-throughput experimentation. Four independent variables that were analyzed were the molecular weight of poly(dimethylsiloxane) (PDMS), presence or absence of poly(epsilon-caprolactone) (PCL) blocks attached to the PDMS backbone, the length of the PCL blocks, and the siloxane polymer level in the coating formulations. In addition to the siloxane libraries (3-aminopropyl-terminated PDMS and poly(epsilon-caprolactone)-poly(dimethylsiloxane)-poly(epsilon-caprolactone) (PCL-PDMS-PCL) triblock copolymers), the coating formulation included a trifunctional isocyanate crosslinker, trifunctional poly(epsilon-caprolactone) polyol, 2,4-pentanedione (pot-life extender), dibutyltin diacetate (catalyst), and a blend of solvents. The resulting coatings were analyzed for their surface energy and pseudobarnacle adhesion both before and after aging the coatings for 30 days in water. The water and methylene iodide contact angle averages increase with increasing molecular weight of PDMS. Coatings prepared from PCL-PDMS-PCL triblock copolymers have lower surface energies than coatings prepared from 3-aminopropyl-terminated PDMS; however, lower pseudobarnacle adhesion results were obtained for the coatings prepared from 3-aminopropyl-terminated PDMS than coatings prepared from PCL-PDMS-PCL triblock copolymers. The siloxane polymer level in the coating formulations does not have a significant effect on the surface energy of the coatings, but it resulted in higher pseudobarnacle adhesion.
The Effect of Protein Kinase C Modulation with Bryostatin 1 on Paclitaxel-Induced Growth Inhibition and Apoptosis in Human Breast Cancer Cell Lines (1999)

Abstract : Polyamines are essential for cell growth and differentiation. Structural polyamine analogs have been shown to have anti-tumor activity in experimental models including breast cancer. This study evaluates the ability of two polyamine analogs, N1-ethyl-N11-((cyclopropyl)methyl)-4,8-diazaundecane (CPENSpm) and N1-ethyl-N11-((cycloheptyl)methyl)4,8-diazaundecane (CHENSpm) to synergize with cytotoxics in five human breast cancer cell lines. The chemotherapeutic agents chosen, cis- diaminechloroplatinum (II), doxorubicin, 5-fluorouracil, fluorodeoxyuridine, 4- hydroperoxycyclophosphamide, paclitaxel, docetaxel, and vinorelbine, all have anti-tumor activity in breast cancer and represent a spectrum of mechanisms. Three treatment schedules of polyamine analog and cytotoxic were tested in MCF-7 and MDA-MB-468 lines. Cytotoxic agent alone for 24 hours followed by polyamine analog alone for 96 hours resulted in the most synergistic combinations and the greatest synergy. Two cytotoxics, vinorelbine and the fluoropyrimidines, showed the most promise in combination with the polyamine analogs. They were able to synergize with one or both polyamine analogs in most of the breast cancer cell lines. CPENSpm was also able to synergize with virtually all cytotoxics in the estrogen receptor positive MCF-7 and T47D lines. These preclinical data demonstrate a treatment schedule and combinations of polyamine analogs and cytotoxics that will be important to study mechanistically and clinically for breast cancer.
Carotenoids, tocopherols and thiols as biological singlet molecular oxygen quenchers (1990)

Singlet molecular oxygen (1O2) has been shown to be generated in biological systems and is capable of damaging proteins, lipids and DNA. The ability of some biological antioxidants to quench 1O2 was studied by using singlet oxygen generated by the thermodissociation of the endoperoxide of 3,3'-(1,4-naphthylidene) dipropionate (NDPO2). The carotenoid lycopene was the most efficient 1O2 quencher (kq + kr = 31 x 10(9) M-1 s-1). Tocopherols and thiols were less effective. The singlet oxygen quenching ability decreased in the following order: lycopene, gamma-carotene, astaxanthin, canthaxanthin, alpha-carotene, beta-carotene, bixin, zeaxanthin, lutein, bilirubin, biliverdin, tocopherols and thiols. However, the compounds with low quenching rate constants occur at higher levels in biological tissues. Thus, carotenoids and tocopherols may contribute almost equally to the protection of tissues against the deleterious effects of 1O2. The quenching abilities of carotenoids and tocopherols were mainly due to physical quenching. In case of some thiols chemical quenching also plays a significant role. Carotenoids and tocopherols have been reported to exert a protective action against some types of cancer.
Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells. (1982)

A porcine genomic clone encoding a major histocompatibility, (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformed lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse beta 2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.
Comparative study of the antigenic composition of oocyst isolates of Cryptosporidium parvum from different hosts (1992)

An investigation was made of the antigenic composition of oocyst isolates of Cryptosporidium parvum by immunoblotting using rabbit polyclonal or murine monoclonal antibodies (MoAbs) developed against this parasite. Using the polyclonal antibodies in blots, a common antigenic profile was obtained from a number of human oocyst isolates from AIDS patients and immunocompetent children in the UK and Portugal. Antigenic differences were observed, however, between a human isolate from Turkey and these other human isolates. The antigenic profiles of oocyst isolates from deer and cattle were similar, but the profiles of the animal and human isolates differed to some extent. Two MoAbs which, in immunofluorescence microscopy, reacted with the surface of the C. parvum sporozoite were also used in blots. A major antigen(s) from 9 of 11 human oocyst isolates recognized by these MoAbs had a molecular weight of 47 kD, but the sizes of the corresponding antigens of the remaining 2 human isolates, one from Turkey (same as above) and one from the UK, were 45.5 and 51 kD, respectively. The equivalent antigen(s) from 4 bovine and 4 ovine isolates was 48 kD. One of the MoAbs failed to react in blots with 2 of the isolates, 1 human and 1 bovine.
Alternativas biotecnológicas para la producción de ácidos grasos poliinsaturados omega-3. (2006)

Fish oils are the main sources of omega-3 (omega 3) polyunsaturated acids (PUFA) such as eicosapentaenoic (C20:5 omega 3) and docosahexaenoic (C22:6 omega 3) acids. World demand for omega 3 PUFA shows an increasing trend mainly due to the growth of the aquaculture industry and also due to the Increasing demand for specific PUFA used as food supplements. Bacteria, fungi, microalgae and thraustochytrids are biotechnological PUFA alternatives to fish oils. These sources are characterized by specific PUFA profiles whose productivity depends on strain and growth conditions. PUFA content in bacteria is low; microalgae synthesize mixtures of PUFA; fungi system productivity is low due to long fermentation times. In heterotrofic cultures of thraustochytrids high concentrations of PUFA can be obtained. Moreover, many strains are able to synthesize a single omega 3. PUFA. The optimization of fermentation systems and the development of technology capable of large-scale production are needed in order to make these alternatives, feasible.
A technique for the study of ultrafast gas–solid reactions for residence times less than 100 ms (1992)

An experimental technique for the study of short contact time gas–solid reactions is developed. The major components are a pulse powder feeder, an injection probe, and a collection probe. The feeder can inject small amounts of fine particles into the reactor as narrow pulses. The two probes also serve as optical solids monitors; an optical system consisting of fiber optic cables and miniature prisms is housed within each probe. With the combination of the two probes, residence time of the particles is determined directly and on-line using photometric principles. With a cold model entrained flow reactor, it is demonstrated that solids with residence times as low as 4 ms can be successfully sampled from the reactor, with the residence time calculated to within 5% error. Methods for rapid heating of the injected powder and rapid quenching of the reaction of the sampled solids are discussed.
A Cinderella Caspase Takes Center Stage (2002)

It has been thought that permeabilization of mitochondria and release of cytochrome c is an ea rly event in apoptosis that is required for activation of caspases, the enzyme executioners of the apoptotic cascade. However, in a Perspective, [Kumar and Vaux][1] explain new findings ([ Lassus et al .][2]) that implicate an often ignored caspase, caspase-2, in the early stages of apoptosis prior to mitochondrial permeabilization.

 [1]: http://www.sciencemag.org/cgi/content/full/297/5585/1290
 [2]: http://www.sciencemag.org/cgi/content/short/297/5585/1352
Planar antennas on a dielectric surface (1981)

A theory has been developed for planar metal antennas on a dielectric surface and the main predictions have been verified experimentally. Current dipole antennas have radiation diagrams and impedances favourable for microwave to far-infra-red integrated circuits.
JSI‐124 (cucurbitacin I) inhibits Janus kinase‐3/signal transducer and activator of transcription‐3 signalling, downregulates nucleophosmin‐anaplastic lymphoma kinase (ALK), and induces apoptosis in ALK‐positive anaplastic large cell lymphoma cells (2006)

JSI-124 (cucurbitacin I) has been recently described as a specific inhibitor of signal transducer and activator of transcription-3 (STAT3). As STAT3 activation is pathogenetically important in anaplastic lymphoma kinase-positive anaplastic large cell lymphoma (ALK+ ALCL), we investigated whether JSI-124 can mediate significant inhibitory effects in this cell type. In two ALK+ ALCL cell lines (Karpas 299 and SU-DHL-1), JSI-124 significantly reduced the number of viable cells to 50% of that of negative controls at a dose of 5-10 micromol/l at 24 h and 1-1.25 micromol/l at 48 h. This decrease in viability was associated with apoptosis, as confirmed by the increase in the subG(0/1) fraction, poly(ADP-ribose)polymerase cleavage and expression of active caspase 3. JSI-124 decreased the phosphorylated-STAT3 and -Janus kinase-3 (JAK3) levels in a dose-dependent fashion, and these changes were coupled with significant decreases in several STAT3 downstream targets, including mcl-1, bcl-2, bcl-xL and cyclin D3. Interestingly, JSI-124 also dramatically decreased the protein levels of JAK3 and nucleophosmin (NPM)-ALK, and these effects were reversible by MG132. Our data support that JSI-124 is a potentially useful therapeutic agent for ALK+ ALCL. In addition to its role as a tyrosine kinase inhibitor, JSI-124 appears to be involved in regulating proteosome degradation for proteins such as JAK3 and NPM-ALK.
Cognitive sequelae of blast-related versus other mechanisms of brain trauma (2009)

The use of improvised explosive devices has become the hallmark of modern warfare and has resulted in an ever-increasing number of blast-related traumatic brain injuries (TBIs). Despite this fact, very little is actually known about the cognitive sequelae of blast-related TBIs. The purpose of the current study was to compare patterns of performance on neuropsychological measures in subjects who have sustained TBIs as a result of blast (or explosion) with those who have sustained TBIs from non-blast or blunt force trauma (motor vehicle accident, fall, assault, etc.). Participants were categorized as blast-related TBI or non-blast-related TBI and according to severity of injury (mild or moderate-to-severe). No main effects were observed in analysis of covariance between blast-related TBI participants and non-blast-related TBI participants across any of the neuropsychological variables, although an interaction was observed on a visual memory test showing stronger performance for mild blast-related and poorer performance for moderate-to-severe blast-related participants compared with both non-blast groups. Overall, the results do not provide any strong evidence that blast is categorically different from other TBI mechanisms, at least with regard to cognitive sequelae on select measures. Additional findings included a marginally increased incidence of reported posttraumatic stress disorder symptoms among blast-injured participants. (JINS, 2009, 15, 1-8.).
Regulation of Leptin Promoter Function by Sp1, C/EBP, and a Novel Factor1 (1998)

Leptin is a hormone produced in adipose cells that regulates energy expenditure, food intake, and adiposity. To understand leptin's transcriptional regulation, we are studying its promoter. Four conserved and functional regions were identified. Mutations in the C/EBP and TATA motifs each caused an approximately 10-fold decrease in promoter activity. The C/EBP motif bound recombinant C/EBP alpha and mediated trans-activation by C/EBP alpha, -beta, and -delta. Mutation of a consensus Sp1 site reduced promoter activity 2.5-fold and abolished binding of Sp1. Mutation of a fourth factor-binding site, denoted LP1, abolished protein binding and reduced promoter activity 2-fold. Factor binding to the LP1 motif was observed with adipocyte, but not with nonadipocyte extracts. Adipocytes from fa/fa Zucker rats transcribed the reporter plasmids more efficiently than did control adipocytes. No effect on the transient expression of leptin was noted upon treatment with a thiazolidinedione, BRL49653, or upon cotransfection with peroxisome proliferator-activated receptor-gamma/retinoid X receptor-alpha or sterol response element-binding protein-1. Mutations of the Sp1, LP1, and C/EBP sites in pairwise combinations diminished promoter activity to the extent predicted assuming these motifs contribute independently to leptin promoter function. Our identification of motifs regulating leptin transcription is an important step in the elucidation of the mechanisms underlying hormonal and metabolic regulation of this gene.
Establishment of a General NAFLD Scoring System for Rodent Models and Comparison to Human Liver Pathology (2014)

We established a simple NAFLD scoring system with high reproducibility that is applicable for different rodent models and for all stages of NAFLD etiology.
The use of spermicide containing nonoxynol-9 in the prevention of HIV infection (1991)

From the Health Education Authority's National HIV Prevention Information Service, London, UK. Kristina Bird, Information Officer, National HIV Prevention Information Service, 82–86 Seymour Place, London W1H 5DB, UK.
영어 유머와 우리말 번역 -로알 달의 작품을 중심으로 (2010)

본 연구의 목적은 영어권 아동문학서에 나타난 유머의 종류와 우리말 번역 시 나타나는 변형 형태를 살피는데 있다. 영어권 아동문학자 중 유머가 풍부한 작품으로 유명하며 국내번역서를 통해 널리 알려진 로알 달(Roald Dahl 1916～1990)의 작품 12종의 원본과 번역서를 통해 분석이 이루어졌다. 작품 속 우스개 말은 거꾸로 말하기, 이름, 동음 반복, 동음이의어, 의성어와 신조어, 틀린 철자와 문법, 과장과 반복, 관용구 바꿔 쓰기, 욕 등으로 구분되었다. 영어 유머의 우리말 번역시 나타난 문제점과 외국어 교육에의 적용을 논의하였다.
Effectiveness of Long-term Acute Care Hospitalization in Elderly Patients With Chronic Critical Illness (2012)

Patients with chronic critical illness transferred to LTACs experience similar survival compared with patients who remain in ICUs, incur fewer health care costs driven by a reduction in postacute care utilization, however, invoke higher overall Medicare payments.
Control of Mitochondrial Morphology Through Differential Interactions of Mitochondrial Fusion and Fission Proteins (2011)

&lt;div&gt;&lt;p&gt;Mitochondria in mammals are organized into tubular networks that undergo frequent shape change. Mitochondrial fission and fusion are the main components mediating the mitochondrial shape change. Perturbation of the fission/fusion balance is associated with many disease conditions. However, underlying mechanisms of the fission/fusion balance are not well understood. Mitochondrial fission in mammals requires the dynamin-like protein DLP1/Drp1 that is recruited to the mitochondrial surface, possibly through the membrane-anchored protein Fis1 or Mff. Additional dynamin-related GTPases, mitofusin (Mfn) and OPA1, are associated with the outer and inner mitochondrial membranes, respectively, and mediate fusion of the respective membranes. In this study, we found that two heptad-repeat regions (HR1 and HR2) of Mfn2 interact with each other, and that Mfn2 also interacts with the fission protein DLP1. The association of the two heptad-repeats of Mfn2 is fusion inhibitory whereas a positive role of the Mfn2/DLP1 interaction in mitochondrial fusion is suggested. Our results imply that the differential binding of Mfn2-HR1 to HR2 and DLP1 regulates mitochondrial fusion and that DLP1 may act as a regulatory factor for efficient execution of both fusion and fission of mitochondria.&lt;/p&gt; &lt;/div&gt;
Increased Vascular Endothelial Growth Factor Levels in Aqueous Humor and Serum of Patients with Quiescent Uveitis (2007)

These findings suggest that VEGF plays a role in uveitis pathogenesis even during inactive disease and that IL-8 levels are significantly influenced by the presence of uveitis-associated extraocular changes.
Determination of the Packed Cell Volume using 131I‐Human Serum Albumin* (1975)

When trace quantities of labelled albumin have been added to blood, the packed cell volume (PCV) may be determined by isotope counting using the relationship: (plasma counts-blood counts)/(plasma counts). This method for measuring the PCV is not affected by plasma trapping between the red cells and the results do not depend upon the centrifugation conditions. The PCV determination with labelled albumin has a high precision (coefficient of variation = 0.9%) and there is very good agreement with the centrifugation PCV using Wintrobe tubes after this measurement has been corrected for plasma trapped between the red cells. No significant compression of the red cells occurred when the blood samples were centrifugated in Wintrobe PCV tubes.
Diagnostic Accuracy and Prognostic Relevance of the Measurement of Cardiac Natriuretic Peptides: A Review (2004)

CNH assays and conventional diagnostic work-ups provide complementary information for evaluation of the presence and severity of cardiac dysfunction and clinical disease. Several aspects of CNH assays are still to be elucidated, and further work is needed to carefully assess their diagnostic accuracy and prognostic value in cardiac disease.
The Role of Atg Proteins in Autophagosome Formation (2011)

Macroautophagy is mediated by a unique organelle, the autophagosome, which encloses a portion of cytoplasm for delivery to the lysosome. Autophagosome formation is dynamically regulated by starvation and other stresses and involves complicated membrane reorganization. Since the discovery of yeast Atg-related proteins, autophagosome formation has been dissected at the molecular level. In this review we describe the molecular mechanism of autophagosome formation with particular focus on the function of Atg proteins and the long-standing discussion regarding the origin of the autophagosome membrane.
The E-CELL project (2000)

Article Free Access Share on The E-CELL project: towards integrative simulation of cellular processes Authors: Masaru Tomita Laboratory for Bioinformatics and Department of Environmental Informatics Laboratory for Bioinformatics and Department of Environmental InformaticsView Profile , Kenta Hashimoto Laboratory for Bioinformatics and School of Media and Governance Laboratory for Bioinformatics and School of Media and GovernanceView Profile , Koichi Takahashi Laboratory for Bioinformatics and School of Media and Governance Laboratory for Bioinformatics and School of Media and GovernanceView Profile , Yuri Matsuzaki Laboratory for Bioinformatics and School of Media and Governance Laboratory for Bioinformatics and School of Media and GovernanceView Profile , Ryo Matsushima Laboratory for Bioinformatics and School of Media and Governance Laboratory for Bioinformatics and School of Media and GovernanceView Profile , Katsuyuki Yugi Laboratory for Bioinformatics and School of Media and Governance Laboratory for Bioinformatics and School of Media and GovernanceView Profile , Fumihiko Miyoshi Laboratory for Bioinformatics and School of Media and Governance Laboratory for Bioinformatics and School of Media and GovernanceView Profile , Hisako Nakano Laboratory for Bioinformatics and Department of Policy Management, Keio University, 5322 Endo, Fujisawa, 252-8520 Japan Laboratory for Bioinformatics and Department of Policy Management, Keio University, 5322 Endo, Fujisawa, 252-8520 JapanView Profile , Yusuke Saito Laboratory for Bioinformatics and Department of Environmental Informatics Laboratory for Bioinformatics and Department of Environmental InformaticsView Profile , S. Shimizu Laboratory for Bioinformatics and Department of Zoology, Cambridge University, CB2 3EJ, UK Laboratory for Bioinformatics and Department of Zoology, Cambridge University, CB2 3EJ, UKView Profile , Yoichi Nakayama Laboratory for Bioinformatics and Department of Environmental Informatics Laboratory for Bioinformatics and Department of Environmental InformaticsView Profile Authors Info & Claims RECOMB '00: Proceedings of the fourth annual international conference on Computational molecular biologyApril 2000 Pages 290–298https://doi.org/10.1145/332306.332567Published:08 April 2000Publication History 1citation569DownloadsMetricsTotal Citations1Total Downloads569Last 12 Months13Last 6 weeks5 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
GCR1‐Dependent Transcriptional Activation of Yeast Retrotransposon Ty2‐917 (1997)

Transcription of Saccharomyces cerevisiae Ty2-917 retrotransposon depends on regulatory elements both upstream and downstream of the transcription initiation site. An upstream activation sequence (UAS) and a downstream enhancer stimulate transcription synergistically. Here we show that activation by both of these sites depends on the GCR1 product, a transcription factor which also regulates the genes encoding yeast glycolytic enzymes. Eliminating GCR1 causes a 100-fold decrease in transcription of Ty2-917. Activation by the isolated Ty2-917 UAS also strongly depends on GCR1. Unexpectedly, GCR1-dependent activation by the Ty2-917 enhancer is strongly position-dependent. Activation by the enhancer in its normal position within the transcription unit depended strongly on GCR1, but eliminating GCR1 reduced activation only three-fold when the enhancer was moved upstream of the transcribed region. Gel mobility shift and DNaseI protection assays indicated that GCR1 binds specifically to multiple sites within the Ty2-917 UAS and enhancer regions. © 1997 John Wiley & Sons, Ltd.
Therapeutic Drug Monitoring of Clozapine: An Unexpected Outcome (2000)

A patient is described who died with the diagnosis of septicemia. After her death the delayed results of a clozapine determination for TDM were sent to the clinician. The clozapine serum level was 4034 microg/L, which was considered to be the primary cause of death. However, a forensic autopsy revealed unexpected metastases of unknown origin with gross liver involvement. Thus the high clozapine levels were judged to be secondary to liver failure. This case is an example of an unexpected outcome of TDM.