gebhart/patrepeval huggingface dataset:
Blown Away: The Unexpected Role of Lymphotoxin in Lymphoid Organ Development (2014)

Lymph nodes (LNs), Peyer’s patches (PPs), and the spleen are command centers for the adaptive immune system, bringing together Ags and immune cells for the initiation of antipathogen offensives. The anatomy of these organs has been studied for over 100 years, and their organization into thymus-
Crystal structures of human cholinesterases in complex with huprine W and tacrine: elements of specificity for anti-Alzheimer's drugs targeting acetyl- and butyryl-cholinesterase (2013)

The multifunctional nature of Alzheimer's disease calls for MTDLs (multitarget-directed ligands) to act on different components of the pathology, like the cholinergic dysfunction and amyloid aggregation. Such MTDLs are usually on the basis of cholinesterase inhibitors (e.g. tacrine or huprine) coupled with another active molecule aimed at a different target. To aid in the design of these MTDLs, we report the crystal structures of hAChE (human acetylcholinesterase) in complex with FAS-2 (fasciculin 2) and a hydroxylated derivative of huprine (huprine W), and of hBChE (human butyrylcholinesterase) in complex with tacrine. Huprine W in hAChE and tacrine in hBChE reside in strikingly similar positions highlighting the conservation of key interactions, namely, π-π/cation-π interactions with Trp86 (Trp82), and hydrogen bonding with the main chain carbonyl of the catalytic histidine residue. Huprine W forms additional interactions with hAChE, which explains its superior affinity: the isoquinoline moiety is associated with a group of aromatic residues (Tyr337, Phe338 and Phe295 not present in hBChE) in addition to Trp86; the hydroxyl group is hydrogen bonded to both the catalytic serine residue and residues in the oxyanion hole; and the chlorine substituent is nested in a hydrophobic pocket interacting strongly with Trp439. There is no pocket in hBChE that is able to accommodate the chlorine substituent.
Rapid and Easy Separation of the Subunits of Bovine and Human Glycoprotein Hormones by Use of High Performance Liquid Chromatography* (1984)

A single method of reverse phase high performance liquid chromatography is used to separate the subunits of human and bovine glycoprotein hormones. This rapid and easy method is applicable for the separation and detection of subunits from as little as 10 micrograms hormone or the isolation of subunits from as much as 100 mg hormone. Separation is achieved by chromatography on a Vydac 218TP1010 column with a linear (60-min) gradient of 0.1 M sodium phosphate, pH 6.8, plus 1 mM sodium azide to a solvent containing 50% acetonitrile and 50% 0.1 M sodium phosphate, pH 6.8, plus 1 mM sodium azide. Although in some cases the interaction between the hydrophobic support and the hormone is sufficient for dissociation, preincubation of the hormone with guanidine hydrochloride ensures optimum dissociation and improves resolution of the subunits. The subunits isolated by high performance liquid chromatography are functional in that they will reassociate with their counterpart subunits.
Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular "heads" of C1q. (1994)

This work describes the functional characterization, cDNA cloning, and expression of a novel cell surface protein. This protein designated gC1q-R, was first isolated from Raji cells and was found to bind to the globular "heads" of C1q molecules, at physiological ionic strength, and also to inhibit complement-mediated lysis of sheep erythrocytes by human serum. The NH2-terminal amino acid sequence of the first 24 residues of the C1q-binding protein was determined and this information allowed the synthesis of two degenerate polymerase chain reaction primers for use in the preparation of a probe in the screening of a B cell cDNA library. The cDNA isolated, using this probe, was found to encode a pre-pro protein of 282 residues. The NH2 terminus of the protein isolated from Raji cells started at residue 74 of the predicted pre-pro sequence. The cDNA sequence shows that the purified protein has three potential N-glycosylation residues and is a highly charged, acidic molecule. Hence, its binding to C1q may be primarily but not exclusively due to ionic interactions. The "mature" protein, corresponding to amino acid residues 74-282 of the predicted pre-pro sequence, was overexpressed in Escherichia coli and was purified to homogeneity. This recombinant protein was also able to inhibit the complement-mediated lysis of sheep erythrocytes by human serum and was shown to be a tetramer by gel filtration in nondissociating conditions. Northern blot and RT-PCR studies showed that the C1q-binding protein is expressed at high levels in Raji and Daudi cell lines, at moderate levels in U937, Molt-4, and HepG2 cell lines, and at a very low level in the HL60 cell line. However, it is not expressed in the K562 cell line. Comparison of gC1q-R NH2-terminal sequence with that of the receptor for the collagen-like domain of C1q (cC1q-R) showed no similarity. Furthermore, antibodies to gC1q-R or an 18-amino acid residue-long NH2-terminal synthetic gC1q-R peptide did not cross-react with antibodies to cC1q-R. Anti-gC1q-R immunoblotted a 33-kD Raji cell membrane protein, whereas anti cC1q-R recognized a molecule of approximately 60 kD. The NH2-terminal sequence of gC1g-R appears to be displayed extracellularly since anti-gC1g-R peptide reacted with surface molecules on lymphocytes, polymorphonuclear leukocytes, and platelets, as assessed by flow cytometric and confocal laser scanning microscopic analyses.(ABSTRACT TRUNCATED AT 400 WORDS)
SEQUENTIAL IMMUNOLOGICAL TARGETING OF CHRONIC EXPERIMENTAL ARTERIAL ALLOGRAFT (1995)

Arterial wall is the main site involved in the chronic rejection process. The rat aortic allograft model was used here to characterize and describe the sequential evolution of the different targets and effectors of arterial wall immunological injury and response during arterial allograft rejection. Rat abdominal aortae were isografted or allografted from Brown-Norway to Lewis rats. Endothelial and smooth muscle cell injury and humoral and cellular immunological effectors were characterized from 0 to 60 days after transplantation using a battery of specific antibodies. The intimal proliferative response was also characterized over this time. Isografted Brown-Norway aorta adventitia had very few cellular components, which suggests that donor adventitia would be poorly antigenic in allografts. In contrast, allograft adventitia was the site of a major inflammatory cell invasion in which the expression of an adhesion molecule by colonizing capillary endothelial cells could play a main role. This adventitial infiltration continued as long as medial smooth muscle persisted. The luminal endothelial cells disappeared early, probably associated with macrophage margination. In contrast, medial smooth muscle cell disappearance occurred later and was specifically targeted by immunoglobulins. Intimal proliferation was the most delayed phenomenon, involving both inflammatory cell infiltration at an early stage and later myofibroblastic proliferation, and could be related to the specific expression of growth factors in this layer. The rat aortic allograft model appeared useful for characterizing specific targets and effectors of chronic arterial graft rejection, demonstrating an early stage of endothelial injury and the presence of immunoglobulins involved in chronic medial smooth muscle cell injury.
Calculation of DEP and EWOD Forces for Application in Digital Microfluidics (2008)

Two primary methods for electrostatically actuating microdroplets in channels currently exist: dielectrophoresis (DEP) for electrically insulating fluids and electrowetting on dielectric (EWOD) for conducting fluids. In each case, a transverse electric field is used to create an electrostatic pressure, giving rise to the transport of individual liquid slugs. This paper examines the nature of the force distribution for both EWOD and DEP actuated droplets. The effects of system parameters such as contact angle and electrode length on the shape of the force density and its net integral are considered. A comparison of the scaling properties of DEP and EWOD for applications in digital microfluidics is presented. The net DEP force is shown to be strongly peaked when a droplet interface is located near the edge of a charged electrode and reduces to the well-known lumped parameter model in the appropriate limits. The effect of electrode spacing is seen to have an inversely proportional effect on the force experienced by the droplet, and the effect of increasing droplet contact angle is observed to increase the net force on the droplet.
Nanotube devices: A microscopic model (2002)

A microscopic model is developed for calculating electrostatic properties of nanotube devices. It is shown that the quantum-mechanical approach yields the same results as the statistical calculation in the limit of a thin tube suspended over a conducting gate at a distance exceeding the nanotube radius. A closed analytic expression is obtained for the atomistic capacitance of a straight nanotube and for a nanotube with a modest curvature. This method allows the fast and exact calculation of device parameters for the nanotube electromechanical systems and nanotube electronic devices.
Breast cancer risk, nightwork, and circadian clock gene polymorphisms (2014)

Night shift work has been associated with an increased risk of breast cancer pointing to a role of circadian disruption. We investigated the role of circadian clock gene polymorphisms and their interaction with nightwork in breast cancer risk in a population-based case-control study in France including 1126 breast cancer cases and 1174 controls. We estimated breast cancer risk associated with each of the 577 single nucleotide polymorphisms (SNPs) in 23 circadian clock genes. We also used a gene- and pathway-based approach to investigate the overall effect on breast cancer of circadian clock gene variants that might not be detected in analyses based on individual SNPs. Interactions with nightwork were tested at the SNP, gene, and pathway levels. We found that two SNPs in RORA (rs1482057 and rs12914272) were associated with breast cancer in the whole sample and among postmenopausal women. In this subpopulation, we also reported an association with rs11932595 in CLOCK, and with CLOCK, RORA, and NPAS2 in the analyses at the gene level. Breast cancer risk in postmenopausal women was also associated with overall genetic variation in the circadian gene pathway (P=0.04), but this association was not detected in premenopausal women. There was some evidence of an interaction between PER1 and nightwork in breast cancer in the whole sample (P=0.024), although the effect was not statistically significant after correcting for multiple testing (P=0.452). Our results support the hypothesis that circadian clock gene variants modulate breast cancer risk.
Facial expression tracking from head-mounted, partially observing cameras (2014)

Head-mounted displays (HMDs) have gained more and more interest recently. They can enable people to communicate with each other from anywhere, at anytime. However, since most HMDs today are only equipped with cameras pointing outwards, the remote party would not be able to see the user wearing the HMD. In this paper, we present a system for facial expression tracking based on head-mounted, inward looking cameras, such that the user can be represented with animated avatars at the remote party. The main challenge is that the cameras can only observe partial faces since they are very close to the face. We experiment with multiple machine learning algorithms to estimate facial expression parameters based on training data collected with the assistance of a Kinect depth sensor. Our results show that we can reliably track people's facial expression even from very limited view angles of the cameras.
Efficacy & safety evaluation of Ayurvedic treatment (Ashwagandha powder & Sidh Makardhwaj) in rheumatoid arthritis patients: a pilot prospective study. (2015)

The findings of the present study suggest that this Ayurvedic treatment (Ashwagandha powder and Sidh Makardhwaj) has a potential to be used for the treatment of rheumatoid arthritis. However, due to small sample size, short duration, non randomization and lack of a control group as study limitations, further studies need to be done to confirm these findings.
Effect of Acidulated Phosphate Fluoride and Casein Phosphopeptide–Amorphous Calcium Phosphate Application on Shear Bond Strength of Orthodontic Brackets (2007)

The use of CPP-ACP either alone or combined with APF could be considered as an alternative prophylactic application in orthodontic practice since it did not compromise bracket bond strength.
Novel Zwitterionic-Polymer-Coated Silica Nanoparticles (2009)

In this work, silica nanoparticles covered with an initiator silane were grafted with novel biocompatible and functionalizable zwitterionic poly[[3-(acryloylamino)propyl](2-carboxyethyl)dimethylammonium] (polyCBAA) via atomic transfer radical polymerization. The stability of these particles in protein solutions and their functionalization were investigated. Dynamic light scattering and transmission electron microscopy were employed to characterize these particles. Results indicate that the polyCBAA-modified nanoparticles are stable for at least 72 h in both negative and positive protein solutions. The size distribution remains the same before and after protein incubation. Moreover, our results show that polyCBAA-modified silica nanoparticles can be easily functionalized. This makes these particles ideal candidates for future applications in targeted drug delivery and diagnostics in complex media.
A Novel Ultrasonic Resonance Field Device for the Retention of Animal Cells (1994)

This article describes two types of flow-through cell retention devices based on the concept of layered piezoelectric resonators. A single-chamber device is compared to a novel optimized steam-sterilizable prototype ultrasonic cell separator with improved acoustic design and an integrated cooling circuit, eliminating the problem of local temperature increase caused by the high amplitudes necessary to achieve the separation of animal cells with low acoustic contrast. This setup yields highly reproducible results and is ideal for studying the long-term effects of ultrasonic sound fields and separation efficiency. The novel two-chamber system has the potential for scaleability due to the reduction in thermal and acoustic flow, increased field stability, and separation efficiency. Finally, the effect of power input on separation and cell viability is reported. Such flow-through cell retention systems could be used as systems to retain biomass within the fermentor or as a substitute for centrifugation, with the major advantage of eliminating high-speed rotational motion.
Study 24: Replication of Tay et al., 2011 (Cell) (2013)

Replication of "Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs.”. Includes information to prepare the replication experiments. Includes data, code, and digital materials from completed replication experiments.
Interaction of Kinetin and Various Inhibitors in the Growth of Soybean Tissue (1966)

Abstract The growth of kinetin‐requiring soybean callus was inhibited by the purine analogs 2,6‐diaminopnrine, 8‐azaguanine and 8‐azaadenine. The purine analog 6‐mercaptopnrine and the antibiotic puromycin were not effective as inhibitors at the concentrations employed. Reversal of the inhibition was attempted by raising the kinetin concentration. Kinetin did not reverse the inhibition due to 2,6‐dianiinopurine but it did lessen to some extent the inhibition due to 8‐azagnanine. It is suggested the kinetin affects RNA metabolism.
Bacteria-induced neo-biosynthesis, stabilization, and surface expression of functional class I molecules in mouse dendritic cells (1998)

Here, we show that bacteria induce de novo synthesis of both major histocompatability complex (MHC) class I and II molecules in a mouse dendritic cell culture system. The neo-biosynthesis of MHC class I molecules is delayed as compared with that of MHC class II. Furthermore, bacteria stabilize MHC class I molecules by a 3-fold increase of their half-life. This has important consequences for the capacity of dendritic cells to present bacterial antigens in the draining lymph nodes. In addition, a model antigen, ovalbumin, expressed on the surface of recombinant Streptococcus gordonii is processed and presented on MHC class I molecules. This presentation is 10(6) times more efficient than that of soluble OVA protein. This exogenous pathway of MHC class I presentation is transporter associated with antigen processing (TAP)-dependent, indicating that there is a transport from phagolysosome to cytosol in dendritic cells. Thus, bacteria are shown to be a potentially useful mean for the correct delivery of exogenous antigens to be presented efficiently on MHC class I molecules.
Hydrogen peroxide electrochemistry on platinum: towards understanding the oxygen reduction reaction mechanism (2012)

Understanding the hydrogen peroxide electrochemistry on platinum can provide information about the oxygen reduction reaction mechanism, whether H(2)O(2) participates as an intermediate or not. The H(2)O(2) oxidation and reduction reaction on polycrystalline platinum is a diffusion-limited reaction in 0.1 M HClO(4). The applied potential determines the Pt surface state, which is then decisive for the direction of the reaction: when H(2)O(2) interacts with reduced surface sites it decomposes producing adsorbed OH species; when it interacts with oxidized Pt sites then H(2)O(2) is oxidized to O(2) by reducing the surface. Electronic structure calculations indicate that the activation energies of both processes are low at room temperature. The H(2)O(2) reduction and oxidation reactions can therefore be utilized for monitoring the potential-dependent oxidation of the platinum surface. In particular, the potential at which the hydrogen peroxide reduction and oxidation reactions are equally likely to occur reflects the intrinsic affinity of the platinum surface for oxygenated species. This potential can be experimentally determined as the crossing-point of linear potential sweeps in the positive direction for different rotation rates, hereby defined as the "ORR-corrected mixed potential" (c-MP).
ALPHA TOXOID OF CLOSTRIDIUM PERFRINGENS (1968)

α-Toxin of Clostridium perfringens was highly sensitive to formalin; its antigenicity was destroyed rapidly during toxoiding. It was succeeded in toxoiding α-toxin under a strict regulation of protein concentration, formalin concentration, constituents of the medium, temperature and incubation period.The ratio of egg unit to mouse toxicity changed during toxoiding and a long incubation was required when the egg yolk reaction was used as a measure for the toxicity. The customary usage of the in vitro test may have been one of the reasons for the inconsistent antigenicities of α-toxoid in the past.
Representing Data Quality for Streaming and Static Data (2007)

In smart item environments, multitude of sensors are applied to capture data about product conditions and usage to guide business decisions as well as production automation processes. A big issue in this application area is posed by the restricted quality of sensor data due to limited sensor precision as well as sensor failures and malfunctions. Decisions derived on incorrect or misleading sensor data are likely to be faulty. The issue of how to efficiently provide applications with information about data quality (DQ) is still an open research problem. In this paper, we present a flexible model for the efficient transfer and management of data quality for streaming as well as static data. We propose a data stream metamodel to allow for the propagation of data quality from the sensors up to the respective business application without a significant overhead of data. Furthermore, we present the extension of the traditional RDBMS metamodel to permit the persistent storage of data quality information in a relational database. Finally, we demonstrate a data quality metadata mapping to close the gap between the streaming environment and the target database. Our solution maintains a flexible number of DQ dimensions and supports applications directly consuming streaming data or processing data filed in a persistent database.
A Grating-Coupler-Enabled CMOS Photonics Platform (2010)

We have developed a silicon photonics platform that allows monolithic integration with electronic circuits in a CMOS-compatible process. In this platform, vertical couplers are found to be a superior solution compared to traditional edge-coupling techniques. Grating couplers are an essential element in developing the optical wafer-scale test infrastructure, which in turn, enables the development of the photonic device library. The photonic devices were assembled into a 4 × 10 Gb/s transceiver die that also contains modulator drive, control, and receive electronics.
Silole Derivatives as Efficient Electron Transporting Materials (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSilole Derivatives as Efficient Electron Transporting MaterialsKohei Tamao, Manabu Uchida, Takenori Izumizawa, Kenji Furukawa, and Shigehiro YamaguchiView Author Information Institute for Chemical Research Kyoto University, Uji, Kyoto 611, Japan Yokohama Research Center Chisso Corporation, Kanazawa-ku Yokohama 236, Japan Cite this: J. Am. Chem. Soc. 1996, 118, 47, 11974–11975Publication Date (Web):November 27, 1996Publication History Received13 August 1996Published online27 November 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja962829chttps://doi.org/10.1021/ja962829crapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views4406Altmetric-Citations401LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Charge transfer,Electrodes,Light,Materials,Reaction products Get e-Alerts