gebhart/patrepeval huggingface dataset:
Multitalker Speech Separation With Utterance-Level Permutation Invariant Training of Deep Recurrent Neural Networks (2017)

In this paper, we propose the utterance-level permutation invariant training (uPIT) technique. uPIT is a practically applicable, end-to-end, deep-learning-based solution for speaker independent multitalker speech separation. Specifically, uPIT extends the recently proposed permutation invariant training (PIT) technique with an utterance-level cost function, hence eliminating the need for solving an additional permutation problem during inference, which is otherwise required by frame-level PIT. We achieve this using recurrent neural networks (RNNs) that, during training, minimize the utterance-level separation error, hence forcing separated frames belonging to the same speaker to be aligned to the same output stream. In practice, this allows RNNs, trained with uPIT, to separate multitalker mixed speech without any prior knowledge of signal duration, number of speakers, speaker identity, or gender. We evaluated uPIT on the WSJ0 and Danish two- and three-talker mixed-speech separation tasks and found that uPIT outperforms techniques based on nonnegative matrix factorization and computational auditory scene analysis, and compares favorably with deep clustering, and the deep attractor network. Furthermore, we found that models trained with uPIT generalize well to unseen speakers and languages. Finally, we found that a single model, trained with uPIT, can handle both two-speaker, and three-speaker speech mixtures.
Saliva as a diagnostic fluid. (1992)

from Venezuela and Malawi, such programmes might also contribute to the first objective-interrupting transmissionby promoting BCG vaccination or revaccination of contacts.2' Achieving the third objective-preventing deformitiesrelies mainly on surveillance to ensure early detection of nerve damage and appropriate treatment for reactions. Until the first two objectives are achieved the paper by ffytche and colleagues will remain relevant.'
Treatment of Anterior Cruciate Ligament Injuries, Part 2 (2005)

Anterior cruciate ligament tears, common among athletes, are functionally disabling; they predispose the knee to subsequent injuries and the early onset of osteoarthritis. A total of 3810 studies published between January 1994 and the present were identified and reviewed to determine the current state of knowledge regarding the treatment of anterior cruciate ligament injuries. Part 1 of this article focused on studies pertaining to the biomechanical behavior of the anterior cruciate ligament, the prevalence of and risk factors for injuries related to it, the natural history of the ligament-deficient knee, injuries associated with anterior cruciate ligament disruption, indications for the treatment of anterior cruciate ligament injuries, as well as nonoperative and operative treatments. Part 2 includes technical aspects of anterior cruciate ligament surgery, bone tunnel widening, graft healing, rehabilitation after anterior cruciate ligament reconstruction, and the effects of sex, age, and activity level on the outcome of such reconstructive surgery.
Topical capsaicin in humans: parallel loss of epidermal nerve fibers and pain sensation (1999)

Capsaicin applied topically to human skin produces itching, pricking and burning sensations due to excitation of nociceptors. With repeated application, these positive sensory responses are followed by a prolonged period of hypalgesia that is usually referred to as desensitization, or nociceptor inactivation. Consequently, capsaicin has been recommended as a treatment for a variety of painful syndromes. The precise mechanisms that account for nociceptor desensitization and hypalgesia are unclear. The present study was performed to determine if morphological changes of intracutaneous nerve fibers contribute to desensitization and hypalgesia. Capsaicin (0.075%) was applied topically to the volar forearm four times daily for 3 weeks. At various time intervals tactile, cold, mechanical and heat pain sensations were assessed in the treated and in contralateral untreated areas. Skin blisters and skin biopsies were collected and immunostained for protein gene product (PGP) 9.5 to assess the morphology of cutaneous nerves and to quantify the number of epidermal nerve fibers (ENFs). Capsaicin resulted in reduced sensitivity to all cutaneous stimuli, particularly to noxious heat and mechanical stimuli. This hypalgesia was accompanied by degeneration of epidermal nerve fibers as evidenced by loss of PGP 9.5 immunoreactivity. As early as 3 days following capsaicin application, there was a 74% decrease in the number of nerve fibers in blister specimens. After 3 weeks of capsaicin treatment, the reduction was 79% in blisters and 82% in biopsies. Discontinuation of capsaicin was followed by reinnervation of the epidermis over a 6-week period with a return of all sensations, except cold, to normal levels. We conclude that degeneration of epidermal nerve fibers contributes to the analgesia accredited to capsaicin. Furthermore, our data demonstrate that ENFs contribute to the painful sensations evoked by noxious thermal and mechanical stimuli.
Secondary structure of substance P bound to liposomes in organic solvents and in solution from Raman and CD spectroscopy. (1990)

The membrane lipid phase may be an important mediator of the peptide-receptor interaction. In order to understand the mechanism of this interaction, it is important to know the peptide structure, not only in the hydrophobic lipid bilayer environment, but also at the bilayer surface and in solution. To investigate this problem we have measured the secondary structure of the 11-residue neuropeptide substance P (SP) and its fragments in aqueous solutions, in membrane mimetic solvents, and associated with lipid bilayers using Raman and CD spectroscopy. Raman and CD spectra of SP bound to liposomes indicate a less than 20% helix content. We interpret these results to indicate that SP contains virtually no helix when bound to negatively charged liposomes. These spectra are similar to spectra of peptides in type I and III beta-turns. SP forms between 10 and 30% (1-3 residues) helical structure in sodium dodecyl sulfate micelles and less than 10% helix in methanol and trifluoroethanol. The binding of SP to negatively charged liposomes significantly changes the structure of the lipid acyl chains, decreasing order in some cases and increasing it in others. Raman spectra of SP in water indicates that SP near 30 mM forms an ensemble of structures in water that is distinct from completely unfolded peptide and from the aggregated beta-sheet form observed in saline solutions. We conclude from our CD results that methods used to quantitate secondary structure from CD spectra of short peptides cannot be used to distinguish between very short helical segments and beta-turns.
Integrated optical Mach-Zehnder biosensor (1998)

We present measurements on biomolecular surface multilayers using an integrated optical sensor based on the Mach-Zehnder interferometer (MZI). The sensor design is unique in that it incorporates a three-waveguide coupler structure at the interferometer output which gives advantages in terms of signal referencing and in establishing and maintaining a sensitive operating point. The sensor performance is characterized with respect to bulk superstrate index and by the formation of multiple protein adlayers using a biotin-avidin-based biochemical system. The detection limit for protein loading is estimated as 5 pg/mm/sup 2/.
The Glycan Domain of Thrombopoietin Enhances Its Secretion (2000)

Thrombopoietin (TPO) is the major regulator of megakaryocyte development and platelet production. The hormone is structurally characterized by an amino-terminal receptor binding domain (amino acid residues 1-152) predicted to encode a left-handed four-helix bundle structure, and a carboxyl-terminal domain (residues 153-335) that is remarkable for its abundant carbohydrate modification and a lack of homology to other proteins. To investigate the functional role of the carboxyl-terminal glycan domain, we generated truncated forms of murine TPO (TPO1-238, TPO1-174, and TPO1-152) and glycomuteins in which the predicted asparagine (N)-linked sites of glycosylation were sequentially mutated to glutamine (Q), and assayed their secretion and function by comparing them to the native sequence (TPO1-335). Following transient transfection of the corresponding cDNA expression vectors into mammalian cell lines, the secretory efficiencies of the proteins were compared with those of the native hormone. Transfection efficiencies were monitored by cotransfection and reporter gene assay, and TPO secretion was assessed by functional and immunologic assays. We found that full-length TPO was 5-29-fold more efficiently secreted than any of the truncated forms of the hormone in fibroblast and hepatocyte cell lines. Elimination of carboxyl-terminal sites of N-linked glycosylation had a minor impact on secretion of the protein. We conclude that the carboxyl-terminal domain of TPO serves the important role of enhancing secretion of the protein, and in this manner functions as a prosequence.
Insect Transgenesis: Current Applications and Future Prospects (2011)

The ability to manipulate the genomes of many insects has become a practical reality over the past 15 years. This has been led by the identification of several useful transposon vector systems that have allowed the identification and development of generalized, species-specific, and tissue-specific promoter systems for controlled expression of gene products upon introduction into insect genomes. Armed with these capabilities, researchers have made significant strides in both fundamental and applied transgenics in key model systems such as Bombyx mori, Tribolium casteneum, Aedes aegypti, and Anopheles stephensi. Limitations of transposon systems were identified, and alternative tools were developed, thus significantly increasing the potential for applied transgenics for control of both agricultural and medical insect pests. The next 10 years promise to be an exciting time of transitioning from the laboratory to the field, from basic research to applied control, during which the full potential of gene manipulation in insect systems will ultimately be realized.
Molecular Mechanisms of Antibody Somatic Hypermutation (2007)

Functional antibody genes are assembled by V-D-J joining and then diversified by somatic hypermutation. This hypermutation results from stepwise incorporation of single nucleotide substitutions into the V gene, underpinning much of antibody diversity and affinity maturation. Hypermutation is triggered by activation-induced deaminase (AID), an enzyme which catalyzes targeted deamination of deoxycytidine residues in DNA. The pathways used for processing the AID-generated U:G lesions determine the variety of base substitutions observed during somatic hypermutation. Thus, DNA replication across the uracil yields transition mutations at C:G pairs, whereas uracil excision by UNG uracil-DNA glycosylase creates abasic sites that can also yield transversions. Recognition of the U:G mismatch by MSH2/MSH6 triggers a mutagenic patch repair in which polymerase eta plays a major role and leads to mutations at A:T pairs. AID-triggered DNA deamination also underpins immunoglobulin variable (IgV) gene conversion, isotype class switching, and some oncogenic translocations in B cell tumors.
Demonstration of an osteoblast defect in two cases of human malignant osteopetrosis. Correction of the phenotype after bone marrow transplant. (1996)

Osteopetrosis is an inherited disorder characterized by bone sclerosis due to reduced bone resorption. Here we report that human osteopetrotic osteoblast-like (Ob) cells express a defective phenotype in primary cultures in vitro, and that bone marrow transplant (BMT) corrects osteoblast function. DNA analysis at polymorphic short-tandem repeat loci from donor, recipient, and primary Ob-like cells pre-BMT and 2 yr post-BMT revealed that Ob were still of recipient origin post-BMT. Osteopetrotic Ob-like cells obtained pre-BMT showed normal and abnormal 1,25(OH)2D3-induced alkaline phosphatase (ALPase) and osteocalcin production, respectively, and failed to produce macrophage colony-stimulating factor (M-CSF) in response to IL-1a and TNF-alpha. These parameters were all normalized in primary Ob-like cells prepared 2 yr post-BMT. X-linked clonality analysis at the human androgen receptor (HUMARA) locus revealed that osteoblasts showed a polyclonal and an oligoclonal derivation pre- and post-BMT respectively, indicating that a limited number of progenitor reconstituted this population. Because osteoblasts were still of recipient origin post-BMT, this suggests that functional osteoclasts, due to the replacement of hematopoeitic cells, provided a local microenvironment in vivo triggering the differentiation and/or recruitment of a limited number of functional osteoblasts.
Freezing of living cells: mechanisms and implications (1984)

Cells can endure storage at low temperatures such as--196 degrees C for centuries. The challenge is to determine how they can survive both the cooling to such temperatures and the subsequent return to physiological conditions. A major factor is whether they freeze intracellularly. They do so if cooling is too rapid, because with rapid cooling insufficient cell water is removed osmotically to eliminate supercooling. Equations have been developed that describe the kinetics of this water loss and permit one to predict the likelihood of intracellular freezing as a function of cooling rate. Such predictions agree well with observations. Although the avoidance of intracellular freezing is usually necessary for survival, it is not sufficient. Slow freezing itself can be injurious. As ice forms outside the cell, the residual unfrozen medium forms channels of decreasing size and increasing solute concentration. The cells lie in the channels and shrink in osmotic response to the rising solute concentration. Prior theories have ascribed slow freezing injury to the concentration of solutes or the cell shrinkage. Recent experiments, however, indicate that the damage is due more to the decrease in the size of the unfrozen channels. This new view of the mechanism of slow freezing injury ought to facilitate the development of procedures for the preservation of complex assemblages of cells of biological, medical, and agricultural significance.
Biocompatibility of hemodialysis membranes. (1990)

Exposure of blood to hemodialysis membranes results in numerous interactions between the blood elements and the membrane. Transformation and adsorption of plasma proteins (such as complement) and activation of blood cells (such as neutrophils and monocytes) have been studied most extensively by nephrologists in recent years. There is no consensus on the definition of biocompatibility for dialyzer membranes. An operational definition of biocompatibility is the lack of any perturbation of blood constituents. According to this "inert surface" definition, a membrane (for example, one that adsorbs beta 2-microglobulin) can be considered as bioincompatible and yet desirable. Because of the multitude of blood-membrane interactions that may occur during hemodialysis, multiple criteria for biocompatibility needs to be applied in the classification of membranes. A certain bioincompatible phenomenon can be further classified as beneficial or deleterious depending on its biological effects as well as its acute and chronic impacts on the dialysis patient.
Using positron emission tomography with [^ F] FDG to predict tumor behavior in experimental colorectal cancer (2001)

This study investigates the relationship between FDG uptake as determined by positron emission tomography (PET) imaging and rates of tumor growth, cellular GLUT1 transporter density, and the activities of hex-okinase and glucose-6-phosphatase in a solid tumor implant model. Five different human colorectal xeno-grafts of different growth properties were implanted in athymic rats and evaluated by dynamic 18F-FDG-PET. The phosphorylating and dephosphorylating activities of the key glycolytic enzymes, hexokinase and glucose-6-phosphatase, were measured in these tumor types by spectrophotometric assays and the expression of GLUT1 glucose transporter protein was determined by immunohistochemistry. Correlations among FDG accu-mulation, hexokinase activity, and tumor doubling time are reported in these colon xenografts. The results indicate that the activity of tumor hexokinase may be a marker of tumor growth rate that can be determined by 18F-FDG-PET imaging. PET scanning may not only be a useful tool for staging patients for extent of disease, but may provide important prognostic information concerning the proliferative rates of malignancies.
Detection of Circulating Tumor Cells in Peripheral Blood of Patients with Metastatic Breast Cancer: A Validation Study of the CellSearch System (2007)

The CellSearch system enables the reliable detection of CTCs in blood and is suitable for the routine assessment of metastatic breast cancer patients in the clinical laboratory. Blood samples should be shipped at room temperature and CTC counts are stable for at least 72 h.
Virtual reality tools for Internet robotics (2002)

A virtual control environment for robot teleoperation via the Internet is presented. It comprises a Java3D-based real-time virtual representation of the robot and worksite, and uses a graphic panel, an environment for remote robot programming, and a dataglove with a 6D position tracker as the control interfaces. The use of virtual reality (VR) techniques for Internet teleoperation allow: (1) time delays inherent in IP networks to be suppressed and (2) the operator's work to be simplified and accelerated, compared to methods that use delayed TV images. The system realisation, with its use of open technologies Java, Java3D and 3-tier client/server architecture, provides portability among different computer platforms and types of robots. The efficiency of the VR-based methods developed has been verified for slow communication rates (0.1-0.5 KB/sec), where TV-based control methods are inapplicable. VR systems have been developed for the WWW-based control of the PUMA and CRS industrial robot manipulators. The particulars of these systems, the experiments undertaken, current issues, and directions of future work are presented.
Cells designed to deliver anticancer drugs by apoptosis. (2002)

We describe a new drug delivery strategy that uses genetically engineered endothelial cells (ECs) to deliver drugs to tumor cells by apoptosis. Immortalized ECs were genetically engineered to express a flk-1:fas fusion protein. When exposed to the flk-1 ligand, vascular endothelial growth factor (VEGF), which is overexpressed by many tumors, these cells underwent extensive apoptosis. These apoptotic ECs, when loaded with drug, promote drug delivery by phagocytosis of drug-loaded apoptotic bodies by the tumor cells and by increased drug transport through the more permeable apoptotic membrane. In the current study, severe combined immunodeficient mice bearing s.c. tumors that expressed high levels of VEGF were treated either intratumorally or i.v. every 4 days for a total of five doses with saline control, free Taxol, and immortalized ECs (imECs) expressing the flk-1:fas fusion protein (imEC/HFF) loaded with Taxol (imEC/HFF-T). Intratumoral treatments also included imEC/HFF and imECs loaded with Taxol (imEC-T). Tumor size was monitored for a minimum of 44 days. Whether administered intratumorally or i.v., imEC/HFF-T cells produced greater inhibition of tumor growth than all other treatments, including Taxol. It was noteworthy that 5 of 16 of the imEC/HFF-T-treated animals were tumor free at the termination of the studies, compared with 2 of 16 animals treated with Taxol. A cell distribution experiment showed that flk-1:fas fusion protein expression ECs as well as parental ECs accumulated in tumor and spleen with the highest level, followed by liver, lung, kidney, and brain. Significant apoptosis of flk-1:fas expression cells was observed in tumor, apparently driven by VEGF secreted from tumor cells. Apoptosis-induced drug delivery offers a new avenue for targeted drug delivery research that uses biological control mechanisms.
Organic Phosphorus Compounds 69 Synthesis and Properties of Cyclic Phosphonate and Thiophosphonate Esters (1976)

Abstract Eighteen cyclic phosphonates and thiophosphonates of the type have been synthesized by the Arbuzov rearrangement or by normal esterification or transesterification procedures and their chemical and spectral properties have been determined.
Asymmetric Catalysis by Alkaloids (1986)

This chapter contains sections titled: Introduction The Chiral Bases—The Cinchona Alkaloids The Cyanohydrin Reaction The Michael Reaction The 1,4-Thiol and -Thiolacetate Additions Selenophenol Addition Reaction Epoxidation of Electron-Poor Olefins Formation of the Phosphorus-Carbon Bond Using Chiral Amine Catalysis 2,2-Cycloaddition Reaction Catalyzed by Quinine Esters 1,2-Additions Conclusions
On the viability of FPGA-based integrated coprocessors (1996)

The paper examines the viability of using integrated programmable logic as a coprocessor to support a host CPU core. This adaptive coprocessor is compared to a VLIW machine in term of both die area occupied and performance. The parametric bounds necessary to justify the adoption of an FPGA-based coprocessor are established. An abstract field programmable gate array model is used to investigate the area and delay characteristics of arithmetic circuits implemented on FPGA architectures to determine the potential speedup of FPGA-based coprocessors. Analysis shows that integrated FPGA arrays are suitable as coprocessor platforms for realising algorithms that require only limited numbers of multiplication instructions. Inherent FPGA characteristics limit the data-path widths that can be supported efficiently for these applications. An FPGA-based adaptive coprocessor requires a large minimum die area before any advantage over a VLIW machine of a comparable size can be realised.
Left Heart Pacing Lead Implantation Using Subxiphoid Videopericardioscopy (2003)

Using subxiphoid videopericardioscopy, uncomplicated, anatomically accurate left heart epicardial pacing lead implantation can be achieved without thoracotomy.