gebhart/patrepeval huggingface dataset:
Modulation of Pulmonary Vascular Resistance As a Target for Therapeutic Interventions in Fontan Patients: Focus On Phosphodiesterase Inhibitors (2012)

Despite advancements in surgical techniques over the past 20 years, Fontan patients have decreased exercise capacity as a consequence of an inherent inability to adequately increase cardiac output during exercise. They are also affected by several complications that are associated with considerable morbidity and mortality. As the systemic and pulmonary circulations are placed in series without a subpulmonary ventricle propelling blood through the pulmonary vasculature, the systemic venous pressure and the respiratory mechanics are the only forces driving pulmonary blood flow. In Fontan circulation, pulmonary vascular resistance is the single most important factor involved in the limitation of cardiac output and treatments able to decrease pulmonary vascular resistance might conversely improve cardiac output and exercise capacity. In this article we discuss the initial experience with the use of sildenafil in Fontan patients and we discuss the possible mechanisms through which sildenafil might positively act in Fontan circulation.
Reduction in microalbuminuria in diabetics by eicosapentaenoic acid ethyl ester (1990)

Eicosapentaenoic acid (EPA) ethyl ester (1.8 g/d) was administered to 16 diabetic patients (5 insulin-dependent and 11 noninsulin-dependent diabetics) for 6 mon. EPA in total plasma fatty acids increased from 4.0 +/- 2.4 mol% (mean +/- SD) to 7.5 +/- 3.1 mol% (p less than 0.001). Albumin excretion, measured with spot urine, was significantly reduced from 65 to 36 mg/g creatinine (geometric means, p less than 0.001). Fasting blood sugar levels, glycohemogloblin, body weight and blood pressure did not change significantly during the study. There were also no significant changes in serum levels of creatinine, urea nitrogen, total cholesterol and triglycerides. Although no overt hemorrhage was observed in the patients, hematocrit was reduced from 42.6 +/- 2.8% to 41.0 +/- 3.9% (p less than 0.02). Ten other similar diabetic patients (4 insulin-dependent and 6 noninsulin-dependent diabetics) were followed as a reference group, not concomitantly, for 6 mon with neither EPA ethyl ester nor placebo. The parameters mentioned above were not changed significantly in this group during 6 mon. EPA administration might retard the appearance of overt diabetic nephropathy.
Mind your Ps and Qs (2008)

Little is known about the impact of politeness in online communities. We use an inductive approach to automatically model linguistic politeness in online discussion groups and determine the impact of politeness on desired outcomes, such as increased reply rates. We describe differences in perceived politeness across a variety of groups and find that, controlling for group norms of responsiveness and message length, politeness increases reply rates in some technical groups, but rudeness is more effective in some political groups. The perceived politeness scores will be used to validate linguistic politeness strategies from theory and to inform the creation of a machine learning model of linguistic politeness that can be applied as a "politeness checker" to educate newcomers to write in ways likely to elicit response from specific communities or as a rudeness detection tool for moderators.
Endothelin-1 Promotes Myofibroblast Induction through the ETA Receptor via a rac/Phosphoinositide 3-Kinase/Akt-dependent Pathway and Is Essential for the Enhanced Contractile Phenotype of Fibrotic Fibroblasts (2004)

The endothelins are a family of endothelium-derived peptides that possess a variety of functions, including vasoconstriction. Endothelin-1 (ET-1) is up-regulated during tissue repair and promotes myofibroblast contraction and migration, hence contributing to matrix remodeling during tissue repair. Here, we show that addition of ET-1 to normal lung fibroblasts induces expression of proteins that contribute to a contractile phenotype, including alpha-smooth muscle actin (alpha-SMA), ezrin, moesin, and paxillin. We confirm that ET-1 enhances the ability of lung fibroblasts to contract extracellular matrix, a function essential for tissue repair, through induction of de novo protein synthesis. Blockade of the Akt/phosphoinositide 3-kinase (PI3-kinase) pathway with LY294002 and wortmannin prevents the ability of ET-1 to induce alpha-SMA, ezrin, paxillin, and moesin and to promote matrix contraction. Dominant negative rac and Akt blocked the ability of ET-1 to promote formation of alpha-SMA stress fibers. Using specific ET-1 receptor inhibitors, we show that ET-1 induces collagen matrix contraction through the ETA, but not the ETB, receptor. Relative to normal pulmonary fibroblasts, fibroblasts cultured from scars of patients with the fibrotic disease systemic sclerosis (scleroderma) show enhanced ET-1 expression and binding. Systemic sclerosis lung fibroblasts show increased ability to contract a collagen matrix and elevated expression of the procontractile proteins alpha-SMA, ezrin, paxillin, and moesin, which are greatly reduced by antagonizing endogenous ET-1 signaling. Thus, blocking ET-1 or the PI3-kinase/Akt cascades might be beneficial in reducing scar formation in pulmonary fibrosis.
Atopy and bronchial hyperresponsiveness: exclusion of linkage to markers on chromosomes 11q and 6p (1992)

Previous studies have reported a familial predisposition for the development of atopy, bronchial hyperresponsiveness and clinical asthma, and therefore have suggested the presence of a heritable component to these disorders. The specific contributions of genetic and environmental factors in the pathogenesis of allergic disease and asthma have not been determined although Cookson et al. [1] have postulated linkage between atopy and chromosome 11q. We have studied 20 families (two and three generations) ascertained through a proband identified as having asthma (90% were also allergic) during the period of time between 1962 and 1970. Of those who were originally skin test positive, 82% remained positive. All probands whose pulmonary function allowed retesting (FEV1 > 1.2 l) remained hyperresponsive to histamine. The children of these probands are now in the same age range as their parents when they were originally evaluated; 66% are atopic using criteria described by Cookson et al. (one or more positive skin tests > or = 2 mm, an elevated total serum IgE or a positive specific IgE) and 22% demonstrate bronchial hyperresponsiveness (PC20 FEV1) to histamine. Using the highly polymorphic marker INT2 (which maps 2 cM from p lambda MS.5 l on chromosome 11q) and atopy, we obtained a lod score of -2.00 at a recombination fraction of 0.12. In addition, because many studies have suggested an association between atopy and certain HLA antigens, we investigated the possibility of linkage between atopy and bronchial hyperresponsiveness and D6S105, a polymorphic marker on chromosome 6p, located 7 cM from HLA-DR. For this marker and atopy, we observed a lod score of -2.00 with a recombination fraction of 0.07.(ABSTRACT TRUNCATED AT 250 WORDS)
C-reactive protein and cardiovascular disease: new insights from an old molecule (2003)

The classical acute-phase protein, C-reactive protein (CRP), is an exquisitely sensitive systemic marker of disease with broad clinical utility for monitoring and differential diagnosis. Inflammation, the key regulator of CRP synthesis, plays a pivotal role in atherothrombotic cardiovascular disease. There is a powerful predictive association between raised serum CRP values and the outcome of acute coronary syndromes, and, remarkably, between even modestly increased CRP production and future atherothrombotic events in otherwise healthy individuals. Baseline CRP values also reflect metabolic states associated with atherothrombotic events. The presence of CRP within most atherosclerotic plaques and all acute myocardial infarction lesions, coupled with binding of CRP to lipoproteins and its capacity for pro-inflammatory complement activation, suggests that CRP may contribute to the pathogenesis and complications of cardiovascular disease. We review the biological properties of CRP, the association between CRP and cardiovascular disease, and the possibility that CRP may be a novel therapeutic target.
Evaluation of cotton as a carrier for solid-phase peptide synthesis. (1992)

Cotton as a new support for the solid-phase synthesis of peptides was evaluated under the conditions of both Boc/Bzl and Fmoc/But strategy. Syntheses of peptides containing only bifunctional amino acids gave comparable results, but peptides containing trifunctional amino acids are clearly advantageously synthesized using the Fmoc approach. The comparison of cotton with other planar carriers (paper or polypropylene membranes) and with classical pellicular ones has shown practical applicability of cotton as the replacement of more sophisticated carriers. N-Methylimidazole was shown to be the optimal acylation catalyst for the modification of the cellulose carrier. Stability of the ester bond to the cotton was studied and shown to be adequate for the syntheses of small to medium size peptides. A relatively low level of substitution of cotton carrier in connection with its ability to soak the solvent was used for the complete elimination of a synthetic vessel. Couplings can be performed by wetting the carrier with the coupling solution, and all solvents can be removed either by squeezing it together with a porous material (paper) or by centrifugation. This leads to a significant decrease of solvent consumption. The efficiency of solvent elimination by centrifugation was demonstrated by the synthesis of model peptide ACP 65-74 in which only one wash between coupling and deprotection and between deprotection and coupling was used and which afforded the product of the same quality as the product synthesized using the standard protocol. Bromophenol blue monitoring has been proven as the optimal way of monitoring the acylation reaction progress. Peptides directly bound to cotton were shown to be useful in enzyme-linked immunosorbent assay tests.
Application and Limits of Sample Stacking in Capillary Electrophoresis (2003)

A blessing and curse of capillary electrophoresis (CE) is the small amount of material that can be injected into the column (, , , ). If one has a tiny quantity of substance to analyze, volumes on the order of 2–10 nL can be injected into a capillary column with good reproducibility; thus, precious material is not wasted. Analyses of material from a single cell have been reported (). However, such a small volume generates a host of difficulties for conventional chromatographic detection technologies. For example, the detection of small volumes using on-column UV detection is hindered by the short optical path defined by the diameter of the column. Although the mass limit in CE can be very low because of the small volume, the concentration limit for UV detection is usually on the order of 10− 6 M, which is several orders higher than detection limits for high-performance liquid chromatography (HPLC). Laser-induced fluorescence allows low concentration detection (on the order of 10−9 M), but most analytes do not fluoresce. Also, most fluorescent labels are not excited by the available laser wavelengths, and laser technology is still complicated and expensive. Thus, one would like a way to concentrate the sample on-column to improve detection without a loss in resolution and analysis time.
Roles of cytotoxic T‐lymphocyte‐associated antigen‐4 in the inductive phase of oral tolerance (2002)

To elucidate the roles of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) in oral tolerance, we studied the consequences of CTLA-4 blockade during the inductive phase of oral tolerance using a transgenic T-cell transfer model. We found that CTLA-4 blockade significantly accelerated cell cycle progression of antigen-specific T cells and dramatically increased their numbers in lymphoid organs following oral administration of ovalbumin (OVA). In mice fed with OVA, only approximately 35% of specific T cells underwent more than four cycles of cell division. This was increased to 65% in mice fed with OVA and treated with a blocking anti-CTLA-4 monoclonal antibody (mAb). The OVA-specific T cells in the latter group were localized primarily in the T-cell zones of the mesenteric lymph nodes and Peyer's patches with a few penetrated into B-cell follicles. Nevertheless, both faecal anti-OVA immunoglobulin A (IgA) and seral anti-OVA immunoglobulin G (IgG) were produced in anti-CTLA-4 mAb-treated mice. These results suggest that CTLA-4 limits the degree of T-cell activation by blocking cell cycle progression during the inductive phase of oral tolerance. In the absence of the CTLA-4 signal, mucosal exposure of antigen induces heightened T-cell activation and expansion, which in turn promotes the production of antigen-specific antibodies.
Pushing the Limits for Thiol−Ene and CuAAC Reactions: Synthesis of a 6th Generation Dendrimer in a Single Day (2010)

Dendrimer synthesis should not be tedious and time-consuming. By utilizing an AB2−CD2 approach and having orthogonal, “clickable” groups on each monomer, the time for dendrimer assembly can be drastically reduced. This was shown by preparation of a sixth generation dendrimer from starting monomer units in a single day.
Increased Plasma Tumor Necrosis Factor in Severe Alcoholic Hepatitis (1990)

Elevations in tumor necrosis factor in alcoholic hepatitis are most marked in severe cases, suggesting that tumor necrosis factor plays a role in the pathogenesis.
Generative Mesh Modeling (2005)

Generative Modeling is an alternative approach for the description of three-dimensional shape. The basic idea is to represent a model not as usual by an agglomeration of geometric primitives (triangles, point clouds, NURBS patches), but by functions. The paradigm change from objects to operations allows for a procedural representation of procedural shapes, such as most man-made objects. Instead of storing only the result of a 3D construction, the construction process itself is stored in a model file. The generative approach opens truly new perspectives in many ways, among others also for 3D knowledge management. It permits for instance to resort to a repository of already solved modeling problems, in order to re-use this knowledge also in different, slightly varied situations. The construction knowledge can be collected in digital libraries containing domain-specific parametric modeling tools. A concrete realization of this approach is a new general description language for 3D models, the "Generative Modeling Language" GML. As a Turing-complete "shape programming language" it is a basis of existing, primitv based 3D model formats. Together with its Runtime engine the GML permits - to store highly complex 3D models in a compact form, - to evaluate the description within fractions of a second, - to adaptively tesselate and to interactively display the model, - and even to change the models high-level parameters at runtime.
Deposition and extensive light soaking of highly pure hydrogenated amorphous silicon (1996)

We have developed an ultrahigh vacuum plasma-enhanced chemical-vapor deposition system, and deposited high-purity device-quality hydrogenated amorphous silicon films. High sensitivity secondary ion mass spectrometry measurements show that impurity contents in the bulk of the present films are reduced to 2×1015 cm−3 for O, 7–10×1015 cm−3 for C, and 5×1014 cm−3 for N; these impurities are normally present at fairly high levels. Nevertheless, extensive light soaking of the films resulted in a defect density as high as 5×1017 cm−3, which is well above the impurity content. This result excludes those models of photoinduced degradation that postulate one-to-one correlation between light-induced defects and O, C, or N impurity atoms.
Paper augmented digital documents (2003)

Paper Augmented Digital Documents (PADDs) are digital documents that can be manipulated either on a computer screen or on paper. PADDs, and the infrastructure supporting them, can be seen as a bridge between the digital and the paper worlds. As digital documents, PADDs are easy to edit, distribute and archive; as paper documents, PADDs are easy to navigate, annotate and well accepted in social settings. The chimeric nature of PADDs make them well suited for many tasks such as proofreading, editing, and annotation of large format document like blueprints.We are presenting an architecture which supports the seamless manipulation of PADDs using today's technologies and reports on the lessons we learned while implementing the first PADD system.
Oligospecificity of the cellular adhesion receptor Mac-1 encompasses an inducible recognition specificity for fibrinogen. (1988)

Mitogenesis, cellular aggregation, and motility follow upon the interaction of fibrinogen with certain defined cell surface receptors. In addition to circulating platelets and vascular endothelium, monocytes express what appears to be a receptor for fibrinogen. Evidence is presented here that the leukocyte adhesion receptor Mac-1 can be specifically induced to bind fibrinogen with characteristics immunochemically and functionally distinct from the established Arg-Gly-Asp-directed fibrinogen receptors. The competence of Mac-1 as a fibrinogen receptor is a general property of cells of monocyte and myeloid lineage acquired after maturational changes of some regions of the alpha subunit of Mac-1 during the process of cell differentiation. This ligand recognition specificity of Mac-1 is lacking for the resting cell. Rather, induction of fibrinogen binding capacity of Mac-1 is due to a cellular response to selected agonists characterized by inducing rapid transients of cytosolic Ca2+. Although different in activation pathways and recognition specificity, Mac-1 exhibits an oligospecific ligand versatility characteristic of other homologous Arg-Gly-Asp-directed adhesion receptors.
Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster. (1992)

The chromo domain was identified as a homologous protein motif between Polycomb (Pc)--a member of the Pc-group genes encoding transcriptional repressors of the homeotic genes--and HP1--a heterochromatin-associated protein encoded by the suppressor of position effect variegation gene Su(var)205. Together with previous genetic studies, this molecular similarity supports the suggestion of a common mechanism used for generating heterochromatin and for repressing homeotic genes. The evolutionary conservation of the chromo domain throughout the animal and plant kingdoms implies an important functional role for this protein motif. We have used transgenic lines as well as transient expression assays employing Drosophila tissue culture cells to study the functional role of the Pc chromo domain. Wild-type Pc protein is endogenously expressed in SL2 cells and is found in large immunologically visible complexes. Mutated Pc proteins were expressed as Pc-beta-galactosidase fusion proteins, and their nuclear distribution was examined by indirect immunofluorescence in tissue culture cells and on polytene chromosomes of transgenic larvae. We show that carboxy-terminal truncations of the Pc protein do not affect chromosomal binding of the fusion protein. However, mutations affecting only the chromo domain including in vitro generated deletions, as well as point mutations, abolish chromosomal binding. Our results demonstrate for the first time that the chromo domain is important for the function of Pc and that it is absolutely required for binding of Pc protein to chromatin. Some of the nuclear patterns generated by the mutated forms of the fusion proteins suggest, furthermore, that the chromo domain could be involved in a packaging mechanism, essential for compacting chromosomal proteins within heterochromatin or heterochromatin-like complexes.
PUF Modeling Attacks on Simulated and Silicon Data (2013)

We discuss numerical modeling attacks on several proposed strong physical unclonable functions (PUFs). Given a set of challenge-response pairs (CRPs) of a Strong PUF, the goal of our attacks is to construct a computer algorithm which behaves indistinguishably from the original PUF on almost all CRPs. If successful, this algorithm can subsequently impersonate the Strong PUF, and can be cloned and distributed arbitrarily. It breaks the security of any applications that rest on the Strong PUF's unpredictability and physical unclonability. Our method is less relevant for other PUF types such as Weak PUFs. The Strong PUFs that we could attack successfully include standard Arbiter PUFs of essentially arbitrary sizes, and XOR Arbiter PUFs, Lightweight Secure PUFs, and Feed-Forward Arbiter PUFs up to certain sizes and complexities. We also investigate the hardness of certain Ring Oscillator PUF architectures in typical Strong PUF applications. Our attacks are based upon various machine learning techniques, including a specially tailored variant of logistic regression and evolution strategies. Our results are mostly obtained on CRPs from numerical simulations that use established digital models of the respective PUFs. For a subset of the considered PUFs-namely standard Arbiter PUFs and XOR Arbiter PUFs-we also lead proofs of concept on silicon data from both FPGAs and ASICs. Over four million silicon CRPs are used in this process. The performance on silicon CRPs is very close to simulated CRPs, confirming a conjecture from earlier versions of this work. Our findings lead to new design requirements for secure electrical Strong PUFs, and will be useful to PUF designers and attackers alike.
Arc flash hazard calculations (2007)

This article discusses a method for generalizing the arc flash calculations for select areas of a power distribution system. The method presented here helps to reduce the number of equipment locations and also calculations which are analyzed using a commercial software package. In an industrial example, this method reduced the number of small feeder locations that required a detailed analysis by 94%. This resulted in a large reduction of onsite data collection, detailed modeling, and arc flash warning labels
Nucleic Acid Aptamers for In Vivo Molecular Imaging (2012)

New drug compounds discovery has moved at an accelerated pace in recent years, with a considerable focus on the transition from in vitro to in vivo models. As a result, there has been a significant increase in the need to adapt and develop novel non-invasive, high resolution in vivo imaging approaches for studying disease development and quantitatively determining molecular and cellular events in vivo. Non-invasive imaging methods allow continuous monitoring of disease development in vivo. For example, real time spatialtemporal analysis of tumour growth can reveal the dynamics of cancer progression. Furthermore, the effects of therapy can be evaluated non-invasively in vivo. These approaches offer the ability to perform repetitive observations and interventions of the biological processes underlying disease development and progression.