gebhart/patrepeval huggingface dataset:
On 802.11 Access Point Locatability and Named Entity Recognition in Service Set Identifiers (2015)

The 802.11 active service discovery mechanism requires the transmission of various attributes in a plain text. These attributes can be collected using passive monitoring and can be used to enumerate the preferred network list (PNL) of client devices. In this paper, we focus on the information that can be obtained using the service set identifiers (SSIDs) that make up the PNL. First, we describe a simple model based on a wireless access point geolocation technique to gauge the potential device locatability using data available on WiGLE.net. Second, we look at additional information that can be extracted from the SSID strings. Our hypothesis is that the entities of potential interest, such as locations and personal names contained within SSIDs, can be recognized in an automated fashion. Using two freely available pretrained named entity recognizers, we were able to identify up to 49% of SSIDs as possibly carrying entities of interest based on multiple data sets. We also show that extracted attributes can be used as an inference basis for additional inference attacks, which presents further opportunities in forensic and intelligence contexts.
Managing design data: the five dimensions of CAD frameworks, configuration management, and product data management (1996)

With the increasing complexity of designs, Design Data Management is regarded as a key enabling technology to achieve higher efficiency in the development of complex products. However, this technology has proven to be surprisingly complex. Commercial solutions in the area of CAD Frameworks, Product Data Management, and Configuration Management Systems currently only solve parts of the total problem, and the acceptance of these systems in industry is still relatively low. In this paper, a model is presented which can be used as a frame of reference for the general problem of managing design data. The model distinguishes between five orthogonal dimensions, which are each, when considered separately, quite simple. In practice, however, two or more dimensions must be handled simultaneously to solve real-world problems. This turns out to be nontrivial because multiple fundamentally different solutions exist for each combination of two or more dimensions. We believe that this 5-D frame of reference can contribute to the creation of better quality solutions in the area of Design Data Management. The 5-D model can also help to explain the familiar phenomenon that a solution which has, for example, proven to be adequate for software development may be rejected by integrated circuit designers, and vice versa.
Simplified 2-Aminoquinoline-Based Scaffold for Potent and Selective Neuronal Nitric Oxide Synthase Inhibition (2014)

Since high levels of nitric oxide (NO) are implicated in neurodegenerative disorders, inhibition of the neuronal isoform of nitric oxide synthase (nNOS) and reduction of NO levels are therapeutically desirable. Nonetheless, many nNOS inhibitors mimic l-arginine and are poorly bioavailable. 2-Aminoquinoline-based scaffolds were designed with the hope that they could (a) mimic aminopyridines as potent, isoform-selective arginine isosteres and (b) possess chemical properties more conducive to oral bioavailability and CNS penetration. A series of these compounds was synthesized and assayed against purified nNOS enzymes, endothelial NOS (eNOS), and inducible NOS (iNOS). Several compounds built on a 7-substituted 2-aminoquinoline core are potent and isoform-selective; X-ray crystallography indicates that aminoquinolines exert inhibitory effects by mimicking substrate interactions with the conserved active site glutamate residue. The most potent and selective compounds, 7 and 15, were tested in a Caco-2 assay and showed good permeability and low efflux, suggesting high potential for oral bioavailability.
Ornithine Decarboxylase Overexpression in Mouse 10T12 Fibroblasts: Cellular Transformation and Invasion (1997)

Our results demonstrate a connection between the polyamine/ODC and the MAP kinase signal transduction pathways and suggest that MAP kinase may play a pivotal role in ODC-induced cell transformation and invasion.
Protein Kinase C ζ (2007)

Our experiments investigated associations of specific isoforms of protein kinase C (PKC) with individual proteins in the cardiac troponin complex. Troponin I (cTnI) associated with PKCepsilon and zeta and troponin T (cTnT) associated with PKC alpha, delta, and epsilon. Based on its association with cTnI, we hypothesized that PKCzeta is a major regulator of myofilament protein phosphorylation. To test this, we infected adult cardiac myocytes with adenoviral constructs containing DsRed monomer-tagged wild type (WT) and the following constitutively active forms of PKCzeta: the pseudo-substrate region (A119E), 3'-phospho-inositide-dependent kinase-1 (T410E), and auto-phosphorylation (T560E). The A119E and T410E mutants displayed increased localization to the Z-discs compared with WT and T560E. Immunoprecipitations were performed in myocytes expressing PKCzeta using PKC phospho-motif antibodies to determine the phosphorylation of cTnI, cTnT, tropomyosin, myosin-binding protein C, and desmin. We did not find serine (Ser) phosphorylation of cTnI or cTnT. However, we observed a significant decrease in threonine (Thr) phosphorylation of cTnI and cTnT notably by PKCzeta T560E. Ser phosphorylation of tropomyosin was increased by all three active mutants of PKCzeta. Ser/Thr phosphorylation of myosin-binding protein C increased primarily by PKCzeta A119E. Both PKCzeta A119E and T410E mutants increased desmin Ser/Thr phosphorylation. To explain the apparent Thr dephosphorylation of cTnI and cTnT, we hypothesized that PKCzeta exists as a complex with p21-activated kinase-1 (Pak1) and protein phosphatase 2A (PP2A), and this was confirmed by immunoprecipitation Western blot. Our data demonstrate that PKCzeta is a novel regulator of myofilament protein phosphorylation.
Calcitonin Gene‐Related Peptide is Chemotactic for Human T Lymphocytes (1992)

Certain neuropeptides, such as CGRP, are associated with C-type nerve fibers in the skin and are known to be proinflammatory mediators. Because of their probable role in various cutaneous diseases, we investigated the effect of alpha- and beta-CGRP on human leukocyte migration in a 48-well microchemotaxis chamber using a 5-microns-pore filter. Elutriated peripheral blood leukocytes (enriched 80-90% for CD3+ and 10-20% for CD20+ lymphocytes) were added to the upper wells, and CGRP to the lower ones in a dose range of 10(-19)-10(-5) M; both were diluted in RPMI medium containing 0.05% fetal calf serum. The chamber was incubated at 37 degrees C for 2.5 hours, and the filter was washed and stained. The mean number of cells migrating through the filter was calculated for quadruplicate wells in each treatment group. Chemotactic activity was expressed as a migration index (MI = number of cells responding to CGRP/media control). Both alpha- and beta-CGRP were optimally chemotactic for leukocytes at approximately 50 pM, with a mean migration index of 11.5 for filter-adherent cells (n = 13 experiments); migration due to chemokinesis was minimal, as measured by checkerboard analysis. Almost all leukocytes that responded to CGRP were T cells (TCs), and the CD4 to CD8 ratio was similar to that of the input population; B cells were not observed. CGRP-induced migration appears to be a specific receptor-mediated event, as pretreating the cells with CGRP resulted in significant down-regulation of their chemotactic response to CGRP, but not to interleukin-1 alpha. Our data suggest that the release of CGRP from free nerve endings near the dermal-epidermal junction could influence cutaneous TC trafficking. As neuropeptides exacerbate (possibly initiate) the inflammatory process, they are likely to be important pharmacological targets in dermatological disorders.
Aerosol gene delivery in vivo. (1992)

The ability to express transgenes selectively within the lung will greatly facilitate the development of gene therapy for a variety of human diseases. We have demonstrated that aerosol administration of a chloramphenicol acetyltransferase (CAT) expression plasmid complexed to cationic liposomes produces high-level, lung-specific CAT gene expression in mice in vivo. Significant levels of CAT activity are seen in the lungs for at least 21 days following aerosolization. In situ immunostaining for intracellular CAT protein reveals that the majority of airway epithelial and alveolar lining cells are transfected in vivo. Histological analyses show no apparent treatment-related damage. These results have important implications for the development of human gene therapy.
The Relationship Between Blood Pressure and Pain (2013)

The relationship between pain and hypertension is potentially of great pathophysiological and clinical interest, but is poorly understood. The perception of acute pain initially plays an adaptive role, which results in the prevention of tissue damage. The consequence of ascending nociception is the recruitment of segmental spinal reflexes through the physiological neuronal connections. In proportion to the magnitude and duration of the stimulus, these spinal reflexes cause the activation of the sympathetic nervous system, which increases peripheral resistances, heart rate, and stroke volume. The response also involves the neuroendocrine system, and, in particular, the hypothalamic-pituitary-adrenal axis, in addition to further activation of the sympathetic system by adrenal glands. However, in proportion to an elevation in resting blood pressure, there is a contemporary and progressive reduction in sensitivity to acute pain, which could result in a tendency to restore arousal levels in the presence of painful stimuli. The pathophysiological pattern is significantly different in the setting of chronic pain, in which the adaptive relationship between blood pressure and pain sensitivity is substantially reversed. The connection between acute or chronic pain and cardiovascular changes is supported observationally, but some of this indirect evidence is confirmed by experimental models and human studies. The pain regulatory process and functional interaction between cardiovascular and pain regulatory systems are briefly reviewed. Various data obtained are described, together with their potential clinical implications.
Fibronectin Type III5 Repeat Contains a Novel Cell Adhesion Sequence, KLDAPT, Which Binds Activated α4β1 and α4β7 Integrins (1997)

The region of fibronectin encompassing type III repeats 4-6 contains a low affinity heparin binding domain, but its physiological significance is not clear. We have studied whether this domain is able to interact with cells as already shown for other heparin binding domains of fibronectin. A computer search based on homologies with known active sites in fibronectin revealed the sequence KLDAPT located in FN-III5. A synthetic peptide containing this sequence induced lymphoid cell adhesion upon treatment with the activating anti-beta1 monoclonal antibody (mAb) TS2/16 or with Mn2+, indicating that KLDAPT was binding to an integrin. A recombinant fragment containing repeat III5 (FN-III5) also mediated adhesion of TS2/16/Mn2+-treated cells while the FN-III6 fragment did not. Soluble KLDAPT peptide inhibited cell adhesion to FN-III5 as well as to a 38-kDa fibronectin fragment and VCAM-1, two previously known ligands for alpha4beta1 integrin. KLDAPT also competed with the binding of soluble alkaline phosphatase-coupled VCAM-Ig to Mn2+-treated alpha4beta1. Furthermore, mAbs anti-alpha4 and anti-alpha4beta7, but not mAbs to other integrins, inhibited cell adhesion to FN-III5 and KLDAPT. These results therefore establish a cell adhesive function for the FN-III5 repeat and show that KLDAPT is a novel fibronectin ligand for activated alpha4 integrins.
Cocaine abuse: neurovascular complications. (1989)

In a review of the records of 3,712 drug abusers, 13 patients were identified with neurologic deficits attributable to the use of cocaine. Ischemic manifestations were the most frequent, occurring in seven (54%) patients, with a mean age of 34.2 years. Three (23%) patients had subarachnoid hemorrhage, and three (23%) had intracerebral hemorrhage. Three patients from other institutions were included in the analysis, for a total of 16 patients, (eight with cerebral ischemia, four with subarachnoid hemorrhage, and four with intracerebral hemorrhage). Of the six patients with head computed tomographic findings of cerebral infarction, five had subcortical infarcts. Two of the four patients who presented with a subarachnoid hemorrhage had a congenital intracranial aneurysm. One of the four patients with an intracerebral hemorrhage had an underlying arteriovenous malformation. The mechanism through which these complications occur is not completely understood. Factors that may play a role include the acute hypertensive response that occurs with cocaine use as well as disordered neurovascular control.
Molecular determinants of reperfusion-induced leukocyte adhesion and vascular protein leakage. (1994)

The adherence and emigration of leukocytes have been implicated as a rate-limiting step in the microvascular dysfunction associated with reperfusion of ischemic tissues. The objective of the present study was to define the relation between leukocyte-endothelial cell adhesion and albumin leakage in rat mesenteric venules exposed to ischemia and reperfusion (I/R). Leukocyte adherence and emigration as well as albumin extravasation were monitored in single post-capillary venules using intravital fluorescence microscopy. Ischemia (0, 10, 15, or 20 minutes) was induced by complete occlusion of the superior mesenteric artery, and all parameters were monitored for 30 minutes after reperfusion. The magnitude of the leukocyte adherence and emigration and albumin leakage elicited by I/R was positively correlated with the duration of ischemia. The albumin leakage response was also highly correlated with the number of adherent and emigrated leukocytes. Monoclonal antibodies against the adhesion glycoproteins CD18, CD11b, intercellular adhesion molecule-1 (ICAM-1) (at 10 and 30 minutes), and L-selectin (at 10 minutes), but not P- or E-selectin, reduced I/R-induced leukocyte adherence and emigration as well as albumin leakage. Platelet-leukocyte aggregates were formed in postischemic venules; the number of aggregates was reduced by antibodies against P-selectin, CD11b, CD18, and ICAM-1, but not E- or L-selectin. These results indicate that reperfusion-induced albumin leakage is tightly coupled to the adherence and emigration of leukocytes in postcapillary venules. This adhesion-dependent injury response is primarily mediated by CD11b/CD18 on activated neutrophils and ICAM-1 on venular endothelium and appears to require L-selectin-dependent leukocyte rolling.
Anesthesia with Abdominal Surgery Leads to Intense REM Sleep during the First Post-operative Week (1990)

Characteristics of nocturnal sleep were investigated in six patients after anesthesia and cholecystectomy and in another six after anesthesia and gastroplasty. All night polysomnographic recordings were obtained while each patient slept in a private surgical ward room through two nights before and five or six nights after operation. Anesthesia included thiopental, N2O, isoflurane, and fentanyl. Postoperative analgesia was provided with parenteral morphine. Other aspects of care were routine. Nocturnal sleep was markedly disturbed after both surgical procedures. Throughout the operative night and subsequent one or two nights, sleep was highly fragmented with the usual recurring cycles of sleep stages completely disrupted. Slow wave sleep was suppressed and rapid eye movement (REM) sleep virtually eliminated. During the following 2-4 nights, as other aspects of sleep recovered, REM sleep reappeared and then increased to greater than the preoperative amount. This increased REM sleep was marked by a heavy density of eye movement activity along with frequent patient reports of unusually distressing dreams or vivid nightmares. It is concluded that anesthesia with upper abdominal surgery leads to a severe disruption of nocturnal sleep followed by the release of highly intense REM sleep about the middle of the first postoperative week.
38.2: Direct Display Interaction via Simultaneous Pen + Multi‐touch Input (2010)

Abstract Current developments hint at a rapidly approaching future where simultaneous pen + multi‐touch input becomes the gold standard for direct interaction on displays. We are motivated by a desire to extend pen and multi‐touch input modalities, including their use in concert, to enable users to take better advantage of each.
Detection of Human Cytomegalovirus DNA by Real-Time Quantitative PCR (2000)

A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.
Micro-Transfer-Printing: Heterogeneous integration of microscale semiconductor devices using elastomer stamps (2014)

Micro-transfer-printing is an emerging method to accurately assemble microscale semiconductor devices onto non-native substrates. In micro-transfer-printing an engineered elastomer stamp coupled to a precision motion controller is utilized to pick-up and transfer arrays of microscale devices. A wide range of materials and devices have been micro-transfer-printed, including; Silicon integrated circuits, Gallium Arsenide LEDs, Gallium Arsenide solar cells, Gallium Arsenide lasers and Gallium Nitride LEDs. Devices compatible with micro-transfer-printing are very thin (< 10 microns) and small (< 100um lateral dimensions) and are ideally suited for making flexible device formats and are also well-suited for wafer-level heterogeneous integration. Here, we review the state-of-the art in Micro-Transfer-Printing and also present Micro-Transfer-Printing as a cost-effective approach to heterogeneous integration of compound semiconductor sensors.
The function of tryptophan residues in membrane proteins (1992)

Membrane proteins have a significantly higher Trp content than do soluble proteins. This is especially true for the M and L subunits of the photosynthetic reaction center from purple bacteria. The Trp residues are not uniformly distributed through the membrane but are concentrated at the periplasmic side of the complex. In addition, Trp residues are not randomly aligned. Within the protein subunits, many form hydrogen bonds with carbonyl oxygens of the main chain, thereby stabilizing the protein. On the surface of the molecule, they are correctly positioned to form hydrogen bonds with the lipid head groups while their hydrophobic rings are immersed in the lipid part of the bilayer. These observations suggest that Trp residues are involved in the translocation of protein through the membrane and that following translocation, Trp residues serve as anchors on the periplasmic side of the membrane.
Surface Display of Recombinant Proteins onBacillus subtilisSpores (2001)

We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression on purified spores. We estimated that more than 1.5 x 10(3) TTFC molecules were exposed on the surface of each spore and recognized by TTFC-specific antibodies. The efficient surface presentation of the heterologous protein, together with the simple purification procedure and the high stability and safety record of B. subtilis spores, makes this spore-based display system a potentially powerful approach for surface expression of bioactive molecules.
A glucagon analog chemically stabilized for immediate treatment of life-threatening hypoglycemia (2014)

For more than half a century glucagon has been used as a critical care medicine in the treatment of life-threatening hypoglycemia. It is commercially supplied as a lyophilized powder intended to be solubilized in dilute aqueous hydrochloric acid immediately prior to administration. We have envisioned a "ready-to-use" glucagon as a drug of more immediate and likely use. Through a series of iterative changes in the native sequence we have identified glucagon analogs of appreciably enhanced aqueous solubility at physiological pH, and of chemical stability suitable for routine medicinal use. The superior biophysical properties were achieved in part through adjustment of the isoelectric point by use of a C-terminal Asp-Glu dipeptide. The native glutamines at positions 3, 20 and 24 as well as the methionine at 27 were substituted with amino acids of enhanced chemical stability, as directed by a full alanine scan of the native hormone. Of utmost additional importance was the dramatically enhanced stability of the peptide when Ser16 was substituted with alpha,aminoisobutyric acid (Aib), a substitution that stabilizes peptide secondary structure. The collective set of changes yield glucagon analogs of comparable in vitro and in vivo biological character to native hormone but with biophysical properties much more suitable for clinical use.
Mycoplasma penetrans sp. nov., from the Urogenital Tract of Patients with AIDS (1992)

An unusual mycoplasma, which was isolated from the urine of a human immunodeficiency virus-positive male homosexual patient, has an elongated flask shape and two unique sharply divided internal compartments. The tiplike compartment is densely packed with fine granules, and the body compartment is loosely filled with coarse granules consistent with ribosomal structures. The organism has properties of adherence, hemadsorption, and cytadsorption and invades many different types of mammalian cells. Adhesion and penetration apparently involve the terminally located tiplike structure. Cholesterol is required for growth, and the mycoplasma ferments glucose and hydrolyzes arginine, but does not hydrolyze urea. The results of DNA homology studies revealed that this organism is not genetically related to previously described mycoplasma species that have the same biochemical properties. The results of serologic studies demonstrated that this organism is antigenically distinct from all previously described mycoplasmas. We propose that this new mollicute species should be named Mycoplasma penetrans sp. nov. The type strain is strain GTU-54-6A1 (= ATCC 55252).