gebhart/patrepeval huggingface dataset:
Toward a theory of organized multimodal integration patterns during human-computer interaction (2003)

As a new generation of multimodal systems begins to emerge, one dominant theme will be the integration and synchronization requirements for combining modalities into robust whole systems. In the present research, quantitative modeling is presented on the organization of users' speech and pen multimodal integration patterns. In particular, the potential malleability of users' multimodal integration patterns is explored, as well as variation in these patterns during system error handling and tasks varying in difficulty. Using a new dual-wizard simulation method, data was collected from twelve adults as they interacted with a map-based task using multimodal speech and pen input. Analyses based on over 1600 multimodal constructions revealed that users' dominant multimodal integration pattern was resistant to change, even when strong selective reinforcement was delivered to encourage switching from a sequential to simultaneous integration pattern, or vice versa. Instead, both sequential and simultaneous integrators showed evidence of entrenching further in their dominant integration patterns (i.e., increasing either their inter-modal lag or signal overlap) over the course of an interactive session, during system error handling, and when completing increasingly difficult tasks. In fact, during error handling these changes in the co-timing of multimodal signals became the main feature of hyper-clear multimodal language, with elongation of individual signals either attenuated or absent. Whereas Behavioral/Structuralist theory cannot account for these data, it is argued that Gestalt theory provides a valuable framework and insights into multimodal interaction. Implications of these findings are discussed for the development of a coherent theory of multimodal integration during human-computer interaction, and for the design of a new class of adaptive multimodal interfaces.
The Role of Fibrinogen in Staphylococcal Adherence to Catheters In Vitro (1990)

A study was conducted to examine the factors involved in adherence of Staphylococcus aureus to various catheter materials. Staphylococcal adherence to catheters coated with fibrinogen was specific and significantly increased compared with that to control catheters (preincubated in phosphate-buffered saline). The adherence of S. aureus to fibrinogen-coated catheters was blocked by goat anti-human fibrinogen antibody, but not fibronectin, vitronectin, or nonimmune goat IgG. Adherence to catheter tubings coated with fibrin did not differ markedly from that to those coated with fibrinogen alone, suggesting the conservation of the staphylococcal binding domain during the conversion of fibrinogen to fibrin. Plasma proteins such as fibronectin or factor XIII that interacted with fibrin did not modify staphylococcal adherence to fibrin. Adherence studies with staphylococcal cells treated with proteinases revealed that staphylococcal surface protein(s) were responsible for fibrinogen binding. These data indicate that fibrinogen or fibrin is the dominant plasma mediator of staphylococcal adherence to intravascular catheters.
Epidemiological Study of the Genetics and Environment of Asthma, Bronchial Hyperresponsiveness, and Atopy (1997)

The Epidemiological Study of the Genetics and Environment of Asthma (EGEA) combined a case-control study and a family study. The total sample of 1,854 consisted of 348 patients with asthma selected through chest clinics and 416 control subjects and nuclear families ascertained through the cases. The protocol included standardized questionnaires, bronchial responsiveness, allergen skin-prick tests according to international protocols, total serum immunoglobulin E (IgE) level measurements, and blood eosinophilia. Criteria used to select subjects with asthma and determine asthma status of relatives for affected sibling pair linkage analysis are described. Based on figures from the 348 asthma cases of the EGEA study, issues relative to the definition of severe asthma and intermediate phenotypes such as bronchial responsiveness and allergic markers are discussed. Given the phenotypic heterogeneity involved, relevant phenotypes that may lead to the detection of genetic factors will depend on the hypothesis tested. Standardization of primary data and subphenotypes is a prerequisite for pooling data, which will be needed in the future to better understand the genetics and environmental factors of asthma.
A designed TLR4/MD-2 complex to capture LPS (2005)

The family of Toll-like receptors (TLRs) is involved in the defense of an organism to microbial attack. TLR4-induced signaling is involved in infectious diseases, chronic inflammatory diseases and sepsis; therefore, we aimed at modulating TLR4-signaling via ligand-binding soluble receptors. Because recognition of microbial structures shows some species-specific traits, we specifically selected the mouse model for later in vivo studies. We first prepared the N-terminally Flag-tagged mouse (m) recombinant (r) soluble (s) fusion proteins mrsTLR4-IgGFc (T4Fc) and mrsMD-2 in Drosophila melanogaster Schneider 2 (S2) cells. The function of these molecules was tested by inhibition of synthesis of pro-inflammatory cytokines after stimulation of mouse macrophage RAW 264.7 cells with purified lipopolysaccharide (LPS). T4Fc alone had no inhibitory activity; however, a T4Fc/MD-2 complex blocked LPS activity. By analogy with `cytokine traps', we then prepared a designer molecule (LPS-Trap) by fusing MD-2 to the C-terminus of soluble TLR4 via a flexible linker. LPS-Trap significantly inhibited TNF production by LPS-stimulated RAW 264.7 cells. Thus, the T4Fc/MD-2 complex as well as the LPS-Trap blocked LPS activity in vitro and might thus represent a new therapeutic option in sepsis by neutralization of TLR4-activating ligands.
Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1 (2003)

&nbsp; Lactobacillus plantarum&nbsp;F1 and&nbsp;L. brevis&nbsp;OG1 isolated from Nigerian fermented food products, produced bacteriocins that had broad spectrum of inhibition against both pathogenic, food spoilage organisms and various lactic acid bacteria.&nbsp; The test organisms exhibited activities of 6400 and 3200 AU/ml respectively against&nbsp;Escherichia coliNCTC10418 and&nbsp;Enterococcus faecalis&nbsp;EF1, but did not inhibit&nbsp;Candida albicansATCC10231 and&nbsp;Klebsiella&nbsp;sp. UCH15. Comparison of the antimicrobial spectra and characterization of the two bacteriocins were not identical. Bacteriocin produced by&nbsp;L. brevis&nbsp;OG1 was the most heat stable at 121oC for 60 min, while that of&nbsp;L. plantarumF1 was stable at 121oC for 10 min. The bacteriocins produced by the test isolates maintained full stability after storage for 60 days at &ndash; 200C; partial stability after storage for 120 days at 40C; while activity was not detected after storage for 80 to 120 days at 370C. Bacteriocin produced by L. brevis OG1 was stable at pH range of 2.0 to 8.0 while, that of&nbsp;L. plantarum&nbsp;F1 was found to be stable at pH 2.0 to 6.0. Their active principle was proteinaceous in nature since the bacteriocins were inactivated by proteolytic enzymes, but not by other non&ndash;proteolytic enzymes. mitomycin C and uv light did not affect the activity of the bacteriocins, while chloroform extraction completely destroyed their activity. Exposure to surfactant resulted in an increase in the bacteriocin titre, except Nonidet P-40, which led to total loss of bacteriocin activity. The bacteriocins were able to pass through cellulose membranes with 100,000 KDa and 1,000,000 KDa but could not pass through one with a 10,000 KDa and 1,000 KDa molecular weight cut off. The paper concluded that the ability of bacteriocins produced by the test isolates in inhibiting a wide-range of bacteria, is of potential interest for food safety and may have future applications as food preservative. Key words:&nbsp;&nbsp;Bacteriocins, lactic acid bacteria, indicator organisms, fermented foods, antagonistic activity.&nbsp;
Isolation of Monoclonal Antibodies Specific for Rous Sarcoma Virus Structural, Polymerase and Transforming Proteins and Their Use for the Study of Mutant Virus-infected Cells (1985)

Monoclonal antibodies were developed that are specific for Rous sarcoma virus structural, polymerase (reverse transcriptase) and transforming proteins. The monoclonal antibodies were shown to bind to purified virus proteins in an indirect 125I-labelled Protein A binding assay suitable for screening even very large numbers of hybridomas. Additional tests for specificity included radioimmunoprecipitation of purified virus structural proteins P12 and P27, of reverse transcriptase subunits alpha and beta, and of the transforming protein pp60v-src. Pilot immunofluorescence and protein kinase assays of the expression of virus proteins in avian and mammalian cells infected by wild-type virus as well as by temperature-sensitive, transformation-defective virus mutants revealed that synthesis of virus structural and transforming proteins is hardly affected by changes in temperature, whereas the pp60v-src-associated kinase activity is temperature-sensitive in cells infected by most, but not all the virus mutants.
Oxygen Pathways and Carbon Dioxide Utilization in Methane Partial Oxidation in Ambient Temperature Electric Discharges (1998)

This methane conversion work studies a plasma reaction system for the production of organic oxygenates. The reactor consists of a glass dielectric interposed between the metal electrodes and a flowing gas stream through which kilovolt ac power with frequencies in the range of 100−200 Hz is applied. The geometry for this amounts to an annular system in which gas flows axially between the electrodes, with one electrode covered by a glass plate. The effect of the glass dielectric is to distribute the microdischarges across the entire electrode area and limit the duration of each microdischarge. The partial oxidation studies in this configuration produce methanol and other oxygenates (formaldehyde, formic acid, methyl formate). Selectivities for these products combined amount to 50−65%. These are the primary products when oxygen is included in the feed to the reactor. Thus far, experiments with low methane conversions have been conducted (up to around 25%) to minimize liquid condensation in the reactor. Byproducts here include significant levels of CO and CO2, but it has been determined that CO2 in the feed inhibits further production of CO2 thereby eliminating net CO2 production while increasing CO selectivity. These results show that oxygen appears to be needed in order to obtain higher methane conversions and significant oxygenated liquid organic products. CO or CO2 does not appear to be a substitute for oxygen when trying to generate these desired oxygenated products.
Combined 5-HT1A and 5-HT1B receptor agonists for the treatment of L-DOPA-induced dyskinesia (2008)

Appearance of dyskinesia is a common problem of long-term l-DOPA treatment in Parkinson's disease patients and represents a major limitation for the pharmacological management of the motor symptoms in advanced disease stages. We have recently demonstrated that dopamine released from serotonin neurons is responsible for l-DOPA-induced dyskinesia in 6-hydroxydopamine (6-OHDA)-lesioned rats, raising the possibility that blockade of serotonin neuron activity by combination of 5-HT(1A) and 5-HT(1B) agonists could reduce l-DOPA-induced dyskinesia. In the present study, we have investigated the efficacy of 5-HT(1A) and 5-HT(1B) agonists to counteract l-DOPA-induced dyskinesia in 1-methyl-4-phenyl 1,2,3,6-tetrahydropyridine (MPTP)-treated macaques, the gold standard model of Parkinson's disease. In addition, we have studied the ability of this treatment to prevent development of l-DOPA-induced dyskinesia in 6-OHDA-lesioned rats. The results demonstrate the existence of a potent synergistic effect between 5-HT(1A) and 5-HT(1B) agonists in their ability to dampen l-DOPA-induced dyskinesia in the MPTP-treated macaques. Sub-threshold doses of the drugs, which individually produced no effect, were able to reduce the abnormal involuntary movements by up to 80% when administered in combination, without affecting the anti-parkinsonian properties of l-DOPA. Furthermore, chronic administration of low doses of the 5-HT(1) agonists in combination was able to prevent development of dyskinesia, and reduce the up-regulation of FosB after daily treatment with l-DOPA in the rat 6-OHDA model. Our results support the importance of a clinical investigation of the effect of 5-HT(1A) and 5-HT(1B) agonists, particularly in combination, in dyskinetic l-DOPA-treated Parkinson's disease patients.
Immunosuppressant Target Protein FKBP12 Is Required for P-Glycoprotein Function in Yeast (1996)

The mammalian P-glycoprotein (Pgp) is a approximately 170-kDa membrane protein that mediates multidrug resistance in many chemotherapy-resistant tumors by effluxing toxic compounds from the cell. Pgp homologs are expressed in many organisms, from bacteria to yeast and mammals. Previous studies established a model system to analyze the function of murine, human, and Plasmodium falciparum Pgp by heterologous expression in the yeast Saccharomyces cerevisiae. However, such studies have been hampered by the inherent resistance of yeast cells to chemotherapeutic agents. We find that an erg6 mutation, which blocks the final synthetic step of the membrane sterol ergosterol, renders yeast sensitive to anthracyclines and dactinomycin, clinically relevant Pgp substrates. We demonstrate that expression of the murine mdr3 gene confers dactinomycin resistance in both the erg6 mutant yeast strain and in an erg6 rad52 DNA repair mutant yeast strain. Similarly, murine mdr3 expression confers resistance to the immunosuppressants cyclosporin A (CsA) and FK506 in a CsA-FK506-sensitive vph6 mutant yeast strain. CsA and FK506 are known to partially overcome Pgp-mediated drug resistance, suggesting the targets of these drugs might regulate Pgp function. We find that both murine mdr3 and the yeast Pgp homolog STE6 function in yeast mutants lacking the CsA target proteins cyclophilin A and calcineurin. In contrast, murine mdr3 function was severely compromised in yeast mutants lacking the FK506/rapamycin target protein FKBP12. Both wild-type FKBP12 and an F43Y FKBP12 mutant with reduced prolyl isomerase activity supported mdr3 function. Our results support the model that immunosuppressants reverse multidrug resistance by competing with other Pgp substrates but reveal that inhibition of FKBP12-dependent Pgp function may also contribute to reversal of multidrug resistance by FK506 and rapamycin.
Vulnerability of Islets in the Immediate Posttransplantation Period: Dynamic Changes in Structure and Function (1996)

To learn more about islet vulnerability in the immediate posttransplant period, 400 syngeneic islets were transplanted under the kidney capsule of B6AF1 mice. Three groups of recipients were used: normal mice (normal), streptozotocin (STZ)-diabetic (diabetic), and STZ-diabetic kept hypo- or normoglycemic with insulin pellets (diabetic-normalized). Normoglycemia was achieved in all three groups 14 days after transplantation; however, in the diabetic and diabetic-normalized groups, blood glucose levels throughout the posttransplantation period were respectively higher and lower than in the normal group. Grafts were harvested 1, 3, 7, and 14 days after transplantation and analyzed for morphology, beta-cell death, beta-cell mass, insulin content, and insulin mRNA expression. In all groups, substantial damage in islet grafts was found on days 1 and 3 with apoptotic nuclei and necrotic cores; on day 3, beta-cell death was significantly higher in the diabetic group than in the other groups. Tissue remodelling occurred in all groups with stable graft appearance on day 14; the actual beta-cell mass of the grafts was lowest in the diabetic group. Graft insulin content decreased in all groups on day 1 and fell even further on days 3 and 7. Insulin mRNA levels of grafts retrieved from both the diabetic and diabetic-normalized group were lower than those from the normal group already by day 1 and remained lower on day 14. In conclusion, the first few days of islet transplantation, even under the most advantageous circumstances of excellent metabolic control, are characterized by dynamic changes, with substantial islet cell dysfunction and death followed by tissue remodelling and then stable engraftment.
Combinatorial Small-Molecule Therapy Prevents Uropathogenic Escherichia coli Catheter-Associated Urinary Tract Infections in Mice (2012)

Catheter-associated urinary tract infections (CAUTIs) constitute the majority of nosocomial urinary tract infections (UTIs) and pose significant clinical challenges. These infections are polymicrobial in nature and are often associated with multidrug-resistant pathogens, including uropathogenic Escherichia coli (UPEC). Urinary catheterization elicits major histological and immunological alterations in the bladder that can favor microbial colonization and dissemination in the urinary tract. We report that these biological perturbations impact UPEC pathogenesis and that bacterial reservoirs established during a previous UPEC infection, in which bacteriuria had resolved, can serve as a nidus for subsequent urinary catheter colonization. Mannosides, small molecule inhibitors of the type 1 pilus adhesin, FimH, provided significant protection against UPEC CAUTI by preventing bacterial invasion and shifting the UPEC niche primarily to the extracellular milieu and on the foreign body. By doing so, mannosides potentiated the action of trimethoprim-sulfamethoxazole in the prevention and treatment of CAUTI. In this study, we provide novel insights into UPEC pathogenesis in the context of urinary catheterization, and demonstrate the efficacy of novel therapies that target critical mechanisms for this infection. Thus, we establish a proof-of-principle for the development of mannosides to prevent and eventually treat these infections in the face of rising antibiotic-resistant uropathogens.
Metabolic Analysis of Escherichia coli in the Presence and Absence of the Carboxylating Enzymes Phosphoenolpyruvate Carboxylase and Pyruvate Carboxylase (2000)

Fermentation patterns of Escherichia coli with and without the phosphoenolpyruvate carboxylase (PPC) and pyruvate carboxylase (PYC) enzymes were compared under anaerobic conditions with glucose as a carbon source. Time profiles of glucose and fermentation product concentrations were determined and used to calculate metabolic fluxes through central carbon pathways during exponential cell growth. The presence of the Rhizobium etli pyc gene in E. coli (JCL1242/pTrc99A-pyc) restored the succinate producing ability of E. coli ppc null mutants (JCL1242), with PYC competing favorably with both pyruvate formate lyase and lactate dehydrogenase. Succinate formation was slightly greater by JCL1242/pTrc99A-pyc than by cells which overproduced PPC (JCL1242/pPC201, ppc(+)), even though PPC activity in cell extracts of JCL1242/pPC201 (ppc(+)) was 40-fold greater than PYC activity in extracts of JCL1242/pTrc99a-pyc. Flux calculations indicate that during anaerobic metabolism the pyc(+) strain had a 34% greater specific glucose consumption rate, a 37% greater specific rate of ATP formation, and a 6% greater specific growth rate compared to the ppc(+) strain. In light of the important position of pyruvate at the juncture of NADH-generating pathways and NADH-dissimilating branches, the results show that when PPC or PYC is expressed, the metabolic network adapts by altering the flux to lactate and the molar ratio of ethanol to acetate formation.
Prostaglandins. IX. Synthesis of (+-)-prostaglandin E1, (+-)-11-deoxyprostaglandins E1,F1.alpha., and F1.beta., and (+-)-9-oxo-13-cis-prostenoic acid by conjugate addition of vinylcopper reagents (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProstaglandins. IX. Synthesis of (+-)-prostaglandin E1, (+-)-11-deoxyprostaglandins E1,F1.alpha., and F1.beta., and (+-)-9-oxo-13-cis-prostenoic acid by conjugate addition of vinylcopper reagentsFrancisco S. Alvarez, Douglas Wren, and Anthony PrinceCite this: J. Am. Chem. Soc. 1972, 94, 22, 7823–7827Publication Date (Print):November 1, 1972Publication History Published online1 May 2002Published inissue 1 November 1972https://pubs.acs.org/doi/10.1021/ja00777a026https://doi.org/10.1021/ja00777a026research-articleACS PublicationsRequest reuse permissionsArticle Views312Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Mutation ofPCDH15among Ashkenazi Jews with the Type 1 Usher Syndrome (2003)

The Usher syndrome, the most frequent cause of deafness and concurrent blindness, occurs with increased frequency in Ashkenazi Jews. The authors of this study demonstrated a founder mutation for the most common form of the type 1 Usher syndrome that probably occurred 350 years ago.
Enzyme-Linked Immunosorbent Assay for Ovine Interferon-τ (1996)

Ovine interferon-tau (OvIFN-tau) is a trophectoderm secretory protein responsible for maintenance of corpus luteum function during early pregnancy. Methods for its quantitation include an antiviral assay and radioimmunoassay (RIA), both of which have disadvantages. We therefore developed an ELISA for OvIFN-tau that is specific, rapid, and 40-fold more sensitive than the current RIA. It uses a monoclonal antibody (Ab) HL129, which binds both native and recombinant (r) OvIFN-tau. The ELISA accurately detected known amounts of rOvIFN-tau added to yeast and tissue culture medium and to sheep serum at concentrations between 0.005 and 50,000 ng/ml. Inter- and intraassay coefficients of variation were 9.3 +/- 0.8 and 6.1 +/- 1.4%, respectively. Data are given for the reliability and reproducibility of the ELISA for measuring native OvIFN-tau and rOvIFN-tau.
Identifying and qualifying reusable software components (1991)

Identification and qualification of reusable software based on software models and metrics is explored. Software metrics provide a way to automate the extraction of reusable software components from existing systems, reducing the amount of code that experts must analyze. Also, models and metrics permit feedback and improvement to make the extraction process fit a variety of environments. Some case studies are described to validate the experimental approach. They deal with only the identification phase and use a very simple model of a reusable code component, but the results show that automated techniques can reduce the amount of code that a domain expert needs to evaluate to identify reusable parts.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>