gebhart/patrepeval huggingface dataset:
A Comparison of the Imaging Performance of High Resolution Ultrasound Scanners for Preclinical Imaging (2011)

Nine ultrasound transducers from six ultrasound scanners were assessed for their utility for preclinical ultrasound imaging. The transducers were: L8-16, L10-22 (Diasus; Dynamic Imaging Ltd., Livingston, UK); L17-5, L15-7io (iU22; Philips, Seattle, WA, USA), HFL38/13-6 (MicroMaxx; Sonosite Inc., Bothell, WA, USA); il3Lv (Vivid 5; GE, Fairfield, CT, USA), RMV 704 (Vevo 770; Visualsonics Inc., Toronto, Canada) and MS550S, MS550D (Vevo 2100; Visualsonics Inc.). A quantitative analysis of the ultrasound images from all nine transducers employed measurements of the resolution integral as an indication of the versatility and technology of the ultrasound scanners. Two other parameters derived from the resolution integral, the characteristic resolution and depth of field, were used to characterise imaging performance. Six of these transducers were also assessed qualitatively by ultrasonically scanning 59 female common marmosets (Callithrix jacchus) yielding a total of 215 scans. The quantitative measurements for each of the transducers were consistent with the results obtained in the qualitative in vivo assessment. Over a 0-10 mm imaging depth, the values of the resolution integral, characteristic resolution and depth of field, measured using the Edinburgh Pipe Phantom, ranged in magnitude from 7-72, 93-930 μm and 3.3-9.2 mm respectively. The largest resolution integrals were obtained using the Vevo 770 and Vevo 2100 scanners. The Edinburgh Pipe Phantom provides a quantitative method of characterising the imaging performance of preclinical imaging scanners.
Anti-HIV Michellamines from Ancistrocladus korupensis (1994)

Here we report details of the isolation and determination of the absolute configurations and comparative anti-HIV activities of novel, atropisomeric naphthylisoquinoline alkaloid dimers, michellamines A, B, and C, from a newly described species of Ancistrocladus from the Korup rainforest of Cameroon. We further provide a more extensive analysis of the range of anti-HIV activity of michellamine B, the most potent and abundant member of the series. Michellamine B inhibited HIV-induced cell killing and viral replication in a variety of human cell lines, as well as in cultures of human peripheral blood leukocytes and monocytes. Michellamine B was active against a panel of biologically diverse laboratory and clinical strains of HIV-1, including the AZT-resistant strain G910-6 and the pyridinone-resistant strain A17; the compound also inhibited several strains of HIV-2.
Prostanoid Receptors: Subtypes and Signaling (2001)

Cyclooxygenases metabolize arachidonate to five primary prostanoids: PGE(2), PGF(2 alpha), PGI(2), TxA(2), and PGD(2). These autacrine lipid mediators interact with specific members of a family of distinct G-protein-coupled prostanoid receptors, designated EP, FP, IP, TP, and DP, respectively. Each of these receptors has been cloned, expressed, and characterized. This family of eight prostanoid receptor complementary DNAs encodes seven transmembrane proteins which are typical of G-protein-coupled receptors and these receptors are distinguished by their ligand-binding profiles and the signal transduction pathways activated on ligand binding. Ligand-binding selectivity of these receptors is determined by both the transmembrane sequences and amino acid residues in the putative extracellular-loop regions. The selectivity of interaction between the receptors and G proteins appears to be mediated at least in part by the C-terminal tail region. Each of the EP(1), EP(3), FP, and TP receptors has alternative splice variants described that alter the coding sequence in the C-terminal intracellular tail region. The C-terminal variants modulate signal transduction, phosphorylation, and desensitization of these receptors, as well as altering agonist-independent constitutive activity.
Computational methods for analysis of human breast tumor tissue in optical coherence tomography images (2006)

Optical coherence tomography (OCT) has been demonstrated as a promising means of identifying the boundaries between normal and diseased breast tissue. This capability has yielded promise for the development of OCT techniques for biopsy guidance, surgical margin assessment, and minimally invasive evaluation of disease states. We present methods for the assessment of human breast tissue based on spatial and Fourier-domain analysis. Derived from preliminary OCT data, these methods are aimed at the development of automated diagnostic tools that will aid in the translation of this technology into the clinical environment.
The receptor tyrosine kinase EphB4 and ephrin-B ligands restrict angiogenic growth of embryonic veins in Xenopus laevis (2000)

The cues and signaling systems that guide the formation of embryonic blood vessels in tissues and organs are poorly understood. Members of the Eph family of receptor tyrosine kinases and their cell membrane-anchored ligands, the ephrins, have been assigned important roles in the control of cell migration during embryogenesis, particularly in axon guidance and neural crest migration. Here we investigated the role of EphB receptors and their ligands during embryonic blood vessel development in Xenopus laevis. In a survey of tadpole-stage Xenopus embryos for EphB receptor expression, we detected expression of EphB4 receptors in the posterior cardinal veins and their derivatives, the intersomitic veins. Vascular expression of other EphB receptors, including EphB1, EphB2 or EphB3, could however not be observed, suggesting that EphB4 is the principal EphB receptor of the early embryonic vasculature of Xenopus. Furthermore, we found that ephrin-B ligands are expressed complementary to EphB4 in the somites adjacent to the migratory pathways taken by intersomitic veins during angiogenic growth. We performed RNA injection experiments to study the function of EphB4 and its ligands in intersomitic vein development. Disruption of EphB4 signaling by dominant negative EphB4 receptors or misexpression of ephrin-B ligands in Xenopus embryos resulted in intersomitic veins growing abnormally into the adjacent somitic tissue. Our findings demonstrate that EphB4 and B-class ephrins act as regulators of angiogenesis possibly by mediating repulsive guidance cues to migrating endothelial cells.
Automatic mapping of nested loops to FPGAS (2007)

This paper present a framework for automatic mapping of perfectly nested loops with constant dependences onto regular processor arrays, suitable for direct implementation on Field Programmable Gate Arrays (FPGAs). The problem is modeled as that of finding a suitable completion procedure for a full-rank linear transformation on the iteration space. The approach enables extraction of necessary degrees of communication-free and pipelined parallelism to optimize performance under the resource constraints of limited logic resources and I/O bandwidth available on an FPGA. The generation of control signals for the custom processing elements is also addressed. Examples of automatic derivation of parallel designs for some common nested loops are provided. Experimental results on the Cray XD1 show that an FPGA-based matrix-multiplication design obtained using the framework attains significant speedup on the XD1's attached FPGA, when compared to execution on the XD1 CPU.
PCR on Cell Lysates Obtained from Whole Blood Circumvents DNA Isolation (2001)

Current methods for PCR use purified genomic DNA, isolated mostly from leukocytes. To avoid time-consuming, and therefore expensive, procedures to purify genomic DNA, methods have been developed that use whole blood or buffy coats for PCR (1)(2)(3)(4)(5). However, extensive washing procedures become necessary to remove hemoglobin and anticoagulants, which inhibit PCR (3)(6)(7). In addition, heat-cool cycles have to be included to liberate DNA from the nucleus (1)(5). Furthermore, PCR protocols are optimal for genomic DNA, but the salt composition of the PCR mixture needs to be readjusted if PCR is performed with whole blood or buffy coats (5)(8). Finally, digestion of PCR products with restriction enzymes may be severely inhibited by anticoagulants (9)(10).

We developed a simplified method to overcome these drawbacks. Briefly, red blood cells are first lysed in a large volume, which dilutes the hemoglobin and anticoagulants to an extent that PCR and post-PCR treatments are not inhibited. The white blood cells are then resuspended in a Tris-EDTA (0.67 mmol/L Tris-HCl, 0.07 mmol/L EDTA, pH 7.5) buffer commonly used to store genomic DNA; this eliminates readjustment of the salt composition of the PCR mixture. Finally, because one freeze-thaw cycle disrupts white blood cells into cellular fragments more efficiently than several heat-cool cycles, storage at −20 °C of white blood cells resuspended in Tris-EDTA should yield, after thawing, a cell lysate suitable for PCR. This assumption was tested using cell lysates for apolipoprotein E ( APOE ) genotyping in a PCR protocol optimized for genomic DNA.

Anonymized cell lysates were prepared from whole-blood samples collected in Vacutainer Tubes (Becton Dickinson) by venipuncture. One set of samples was collected from 24 consecutive different patients referred for routine clinical chemistry laboratory investigation; these samples …
Galectin-1 serum levels reflect tumor burden and adverse clinical features in classical Hodgkin lymphoma (2013)

Galectin-1 (Gal1) is a member of a highly conserved family of carbohydrate-binding proteins. It modulates innate and adaptive immune responses and fosters tumor-immune escape. Hodgkin lymphoma (HL) Reed-Sternberg cells overexpress and secrete Gal1, which selectively kills T helper (Th)1 and Th17 cells and cytotoxic T cells and promotes the immunosuppressive Th2/regulatory T-cell-predominant HL microenvironment. We developed a sandwich enzyme-linked immunosorbent assay and assessed serum Gal1 levels in 293 newly diagnosed, previously untreated patients with classical HL (cHL) enrolled in 3 risk-adapted clinical trials. Serum Gal1 levels were significantly higher in patients with cHL than in normal controls (P < .0001). Gal1 serum levels also increased with Ann Arbor stage (P = .012), areas of nodal involvement (P < .0001), and the International Prognostic Score (2-7, P = .019). We conclude that Gal1 serum levels are significantly associated with tumor burden and related clinical features in newly diagnosed cHL patients.
Transforming growth factor production by chemically transformed cells. (1981)

Evidence is presented indicating that the chemically transformed AKR-MCA and C3H/MCA-58 murine cell lines produce "transforming growth factor(s)" capable of inducing a transformed morphology and the ability to grow in soft agar in nontransformed, anchorage-dependent indicator cells. Serum-free medium conditioned by exposure to the chemically transformed cells was chromatographed on a Bio-Gel P-60 column after dialysis and lyophilization. Using the nontransformed mouse AKR-2B cells as the indicator cells, a peak of soft agar growth-stimulating activity was detected in the molecular weight range of 10,000 to 12,000. The soft agar growth-stimulating activity in pooled fractions from the AKR-MCA cells was shown to be trypsin and dithiothreitol sensitive and relatively heat stable; the activity was not destroyed by heating to 56 degrees for 30 min or to 100 degrees for 3 min. The pooled material also caused stimulation of growth in the soft agar of rat NRK cells and stimulation of DNA synthesis in the AKR-2B cells. The quantity required to give significant competition for binding to the epidermal growth factor receptor was about one order of magnitude greater than that required for stimulation of soft agar growth. Further separation of these polypeptide(s) by carboxymethylcellulose chromatography revealed three apparent peaks of soft agar growth-stimulating activity. Epidermal growth factor receptor-competing activity cochromatographed with the early minor soft agar growth-stimulating peak, whereas the two major peaks of soft agar growth-stimulating activity had no associated detectable competition for epidermal growth factor binding to its receptor. The data indicate that at least a major portion of the transforming growth factors produced by the chemically transformed cells is different from those described previously in murine sarcoma virus-transformed mouse cells and human tumor cells.
Continuous Multistep Microfluidic Assisted Assembly of Fluorescent, Plasmonic, and Magnetic Nanostructures (2013)

Lab-on-a-particle: Fluorescent, plasmonic, and magnetic SiO2*-Au-γ-Fe2O3 nanostructures were assembled under continuous flow using two microfluidic devices (μR1 and μR2) connected in series. After assembling the SiO2*-Au nanostructures by electrostatic interactions, γ-Fe2O3 nanoparticles were attached to the structures (see picture). As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Composition of soybean lecithin (1981)

Abstract Commercial soybean lecithin is a complex mixture containing ca. 65–75% phospholipids together with triglycerides and smaller amounts of other substances. The major phospholipids include phosphatidylcholine, phosphatidylethanolamine and inositol‐containing phosphatides. Other substances reported include carbohydrates, pigments, sterols and sterol glycosides. This paper reviews the nature of the compounds found in soybean lecithin and our present knowledge of its composition.
Three-dimensional Mach-Zehnder interferometer in a microfluidic chip for spatially-resolved label-free detection (2010)

Ultrafast laser writing of waveguides in glasses is a very flexible and simple method for direct on-chip integration of photonic devices. In this work we present a monolithic optofluidic device in fused silica providing label-free and spatially-resolved sensing in a microfluidic channel. A Mach-Zehnder interferometer is inscribed with the sensing arm orthogonally crossing the microfluidic channel and the reference arm passing over it. The interferometer is integrated either with a microchannel fabricated by femtosecond laser technology or into a commercial lab-on-chip for capillary electrophoresis. The device layout, made possible by the unique three-dimensional capabilities of the technique, enables label-free sensing of samples flowing in the microchannel with spatial resolution of about 10 microm and limit of detection down to 10(-4) RIU.
Systems of Coupled Oscillators (2005)

This chapter contains sections titled: Mutually Coupled Oscillators Using the Classical Pendulum Analogy Phase Condition for Mutually Locked (Synchronized) Coupled Oscillators Dynamics of Coupled Oscillators Dynamics of N-coupled (Synchronized) Oscillators Oscillator Noise Noise Analysis of the Uncoupled Oscillator Noise Analysis of Mutually Coupled (Synchronized) Oscillators Noise Analysis of N-coupled (Synchronized) Oscillators N-push Coupled Mode (Synchronized) Oscillators Ultra-low-noise Wideband Oscillators
Intrusion detection systems and multisensor data fusion (2000)

of data from myriad heterogeneous distributed network sensors to effectively create cyberspace situational awareness.
Antigen Peptide-Based Immunosensors for Rapid Detection of Antibodies and Antigens (2009)

The homogeneous immunosensor design described here utilizes the bivalent nature of the antibody. Antigen peptide is conjugated using flexible linkers with short complementary oligonucleotides (signaling oligonucleotides), each of which containing a fluorochrome that can form a fluorescence resonance energy transfer (FRET) donor-acceptor pair. The complementary signaling oligonucleotides are short enough to prevent their annealing on their own. Binding of the peptide-signaling oligonucleotide constructs to bivalent antibody results in a large increase in local concentration of signaling oligonucleotides causing their annealing and appearance of FRET signal. We used simple model system (antibiotin antibody) to obtain proof-of-principle validation of the sensor design. We then constructed two sensors based on two peptides corresponding to the antigens of two antibodies raised against human cardiac troponin I. We demonstrated that these sensors could be used for sensitive detection of the antibody and for competition-based detection of the intact troponin I. Furthermore, we showed that these sensors could be used for detection of kinase activity targeting the antigen peptide. These simple and robust immunosensors may find applications in antibody detection (for example, in diagnosis of autoimmune or infectious disease), in protein detection (especially when speed of detection is essential), and in assays for detecting enzymatic activities involved in post-translational modifications of proteins.
Specific regulation of VLA-4 and α 4 β 7 integrin expression on human activated T lymphocytes (1996)

Modulation of VLA integrins was studied in several human T cell clones upon specific and nonspecific cellular activation. Human activated T lymphocytes down-regulated both alpha 4 beta 1 and alpha 4 beta 7 integrins upon specific recognition of alloantigens (cytotoxic T cells) or in the presence of Staphylococcus enterotoxin B (superantigen recognizing noncytotoxic T cells). In contrast, the expression of other membrane integrins, such as VLA-1 and VLA-5 integrins, was not modified. Down-regulation of alpha 4 beta 1 and alpha 4 beta 7 integrins was observed as early as 3 h after stimulation, lasted later than 72 h and was partially inhibited by cytochalasin D. Interestingly, neither target cells nor NK cells modulated CD49d expression after interaction with T cells of K562, respectively, suggesting that CD49d expression was linked to specific T cell activation. The down-regulation of the CD49d chain in T cell clones stimulated with immobilized anti-CD3 mAbs confirmed the role of TCR-mediated activation in CD49d regulation. However, the CD3-independent cellular aggregation induced by soluble anti-CD43 mAb was also able to strongly down-regulate alpha 4 beta 1 and alpha 4 beta 7. The present work shows the first evidence that CD49d subunit-bearing integrin expression is distinctly regulated from other integrins after Ag or superantigen recognition by human activated T cells. CD49d modulation may be relevant for the traffic and tissue localization of locally activated T cells during immune responses.
Roles of epithelial cell-derived periostin in TGF-β activation, collagen production, and collagen gel elasticity in asthma (2010)

Periostin is considered to be a matricellular protein with expression typically confined to cells of mesenchymal origin. Here, by using in situ hybridization, we show that periostin is specifically up-regulated in bronchial epithelial cells of asthmatic subjects, and in vitro, we show that periostin protein is basally secreted by airway epithelial cells in response to IL-13 to influence epithelial cell function, epithelial-mesenchymal interactions, and extracellular matrix organization. In primary human bronchial epithelial cells stimulated with periostin and epithelial cells overexpressing periostin, we reveal a function for periostin in stimulating the TGF-beta signaling pathway in a mechanism involving matrix metalloproteinases 2 and 9. Furthermore, conditioned medium from the epithelial cells overexpressing periostin caused TGF-beta-dependent secretion of type 1 collagen by airway fibroblasts. In addition, mixing recombinant periostin with type 1 collagen in solution caused a dramatic increase in the elastic modulus of the collagen gel, indicating that periostin alters collagen fibrillogenesis or cross-linking and leads to stiffening of the matrix. Epithelial cell-derived periostin in asthma has roles in TGF-beta activation and collagen gel elasticity in asthma.
Pathologic findings from the National Surgical Adjuvant Breast Project (NSABP) eight-year update of Protocol B-17 (1999)

The degree of comedo necrosis in patients with DCIS appears to be sufficient for discriminating between high and low risks for IBT following lumpectomy for DCIS. Although margin status, unlike in our previous report, was found to have only a slight or borderline influence on the frequency of IBT at 8 years, excision of DCIS with free margins is advised. The low risk group exhibits a statistically significant reduction of IBT from XRT. The decision to forgo XRT in the treatment of this singular subset of patients would appear to depend on clinical considerations and the input of informed patients rather than being standard practice. [See editorial on pages 375-7, this issue.]
W-2W Current Steering DAC for Programming Phase Change Memory (2009)

This paper presents the design and experimental results of W-2W current mirror binary-weighted current steering digital-to-analog converter (DAC) and its application for programming phase change memory (PCM). This new approach significantly reduces the layout area of current-mode DACs by the virtue of its compact size. The proposed DAC can replace the write driver circuits in phase change memories. Both 6-bit and 12-bit DACs have been fabricated in 0.5 mum CMOS technology. The layout size of the 12-bit and the 6-bit DACs is 0.09 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and 0.04 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> respectively. Experimental results are presented and the limitations are discussed.
Educating for the Gaming Industry: Need, Profile, and Suggested Schema (2012)

As legalized gambling becomes increasingly widespread and competitive, operators seek employees who understand its unique culture and demands. Today, few baccalaureate programs offer gaming management education. Gaming subject areas for college programs span three content spheres: (a) gaming and games themselves; (b) gaming interfaces with other hospitality subjects (lodging, accounting); and (c) the gaming/hospitality environment (regulation, economics). In a given academic program, gaming content integration can be measured along a continuum, from nonexistent, to somewhat integrated within existing courses, to comprising a central educational focus.
Safety and efficacy of a multicenter study using intraarterial chemotherapy in conjunction with osmotic opening of the blood-brain barrier for the treatment of patients with malignant brain tumors (2000)

Using standard guidelines and protocols, intraarterial chemotherapy with or without osmotic opening of the BBB is feasible across multiple centers with a low incidence of catheter-related complications. In patients with chemotherapy-sensitive tumors, such as PCNSL, PNET, germ cell tumor, and cancer metastasis to the central nervous system, enhanced delivery results in a high degree of tumor response, with an efficacy profile that is reproducible across multiple centers.
1989 (1992)

Wind chill figures will be heard in most radio newscasts this month—at least in the parts of the country that have a winter. If you have a computer and punch in the little program given here, you will be able to check the experts.