gebhart/patrepeval huggingface dataset:
Impaired cholinergic function in cell lines derived from the cerebral cortex of normal and trisomy 16 mice (2000)

Abstract Murine trisomy 16 is an animal model of human Down's syndrome. We have successfully established permanently growing cell lines from the cerebral cortex of normal and trisomy 16 foetal mice using an original procedure. These lines, named CNh (derived from a normal animal) and CTb (derived from a trisomic foetus), express neuronal markers. Considering that Down's syndrome exhibits cholinergic deficits, we examined cholinergic function in these lines, using incorporation of [ 3 H]‐choline and fractional release studies. After 1, 3 and 5 min of [ 3 H]‐choline incubation, CTb cell uptake was lower by ∼ 50% compared to controls. Hemicholinium‐3 significantly reduced the incorporation of [ 3 H]‐choline in both CNh and CTb cells at high concentration (10 μ m ), suggesting high‐affinity choline transport. However, CTb cells exhibited greater sensitivity to the blocker. For fractional release experiments, the cells were stimulated by K + depolarization, glutamate or nicotine. When depolarized, CTb cells showed a 68% reduction in fractional release of [ 3 H]‐acetylcholine compared to CNh cell line, and a 45% reduction when stimulated by nicotine. Interestingly, glutamate induced similar levels of release in both cell types. The results indicate the existence of cholinergic dysfunction in CTb cells when compared to CNh, similar to that reported for primary cultures of trisomy 16 brain tissue ( Fiedler et al . 1994 , Brain Res., 658, 27–32). Thus, the CTb cell line may serve as a model for the study of Down's syndrome pathophysiology.
Primer dosing of S. typhimurium A1-R potentiates tumor-targeting and efficacy in immunocompetent mice. (2013)

We developed the tumor-targeting strain Salmonella typhimuium A1-R (A1-R) and have shown it to be active against a number of tumor types in nude mice. However, in immunocompetent mice, dosing of A1-R has to be adjusted to avoid toxicity. In the present study, we developed a strategy to maximize efficacy and minimize toxicity for A1-R tumor-targeting in immunocompetent mice implanted with the Lewis lung carcinoma. A small primer dose of A1-R was first administered (1×10(6) colony forming unit [cfu] i.v.) followed by a high dose (1×10(7) cfu i.v.) four hours later. The primer-dose strategy resulted in smaller tumors and no observable side-effects compared to treatment with high-dose-alone. The serum level of tumor necrosis factor (TNF-α) was elevated in the mice treated with primer dose compared to mice only given the high dose. Tumor vessel destruction was enhanced by primer dosing of A1-R in immuno-competent transgenic mice expressing the nestin-driven green fluorescent protein, which is selectively expressed in nascent blood vessels. The primer-dose may activate TNF-α and other cytokines in the mouse, necessary for invasion of the tumor by the bacteria, as well as enhance tumor vessel destruction, thereby allowing a subsequent therapeutic dose to be effective and safe. The results of the present study suggest effective future clinical strategies of bacterial treatment of cancer.
A simple method of calculating the interaural cross-correlation function for a sound field (1993)

A simple equation for calculating the interaural cross-correlation function at the two ears of a dummy head for octave bandpass-filtered noise (125 Hz–8 kHz) was derived. This can be applied for any single sound direction. Superposing such cross-correlation functions (weighted with the spectrum of a source signal), the interaural cross-correlation function and its magnitude for a sound field with multiple reflections may be calculated. This is called the IACC for music and continuous speech. Agreement of calculated IACCs with measured ones is satisfactory for these sound sources. When IACC values are measured with a dummy head and with real human heads, the difference in the size of heads is not critical.
Femtosecond laser pulse irradiation of solid targets as a general route to nanoparticle formation in a vacuum (2005)

By studying the fs laser produced plume of different materials we show experimentally that the process of matter removal during ultrashort (fs) laser pulse irradiation followed by vacuum expansion is characterized by a number of general features, whichever the nature of the target material. In particular, fs laser ablation of solid targets at laser intensities of the order of ${10}^{12}\ensuremath{-}{10}^{13}\phantom{\rule{0.3em}{0ex}}\mathrm{W}∕{\mathrm{cm}}^{2}$, inevitably leads to the generation of nanoparticles of that material. This has been evidenced by atomic force microscopy analysis of less than one layer deposits showing that the produced nanoparticles have mean radii generally in the range 5--25 nm, with pretty narrow size distributions. These results are in very good agreement with the physical description and numerical predictions of recently published theoretical analyses of fs ablation processes.
X anthomonas campestris pv. campestris (cause of black rot of crucifers) in the genomic era is still a worldwide threat to brassica crops (2012)

http://www.xanthomonas.org/
Synthesis of acylhydrazido-substituted cephems. Design of cephalosporin-vinca alkaloid prodrugs: substrates for an antibody targeted enzyme (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of acylhydrazido-substituted cephems. Design of cephalosporin-vinca alkaloid prodrugs: substrates for an antibody targeted enzymeLouis N. Jungheim, Timothy A. Shepherd, and Damon L. MeyerCite this: J. Org. Chem. 1992, 57, 8, 2334–2340Publication Date (Print):April 1, 1992Publication History Published online1 May 2002Published inissue 1 April 1992https://pubs.acs.org/doi/10.1021/jo00034a027https://doi.org/10.1021/jo00034a027research-articleACS PublicationsRequest reuse permissionsArticle Views544Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Low-resolution ADC receiver design, MIMO interference cancellation prototyping, and PHY secrecy analysis. (2017)

This dissertation studies three independent research topics in the general field of wireless communications. The first topic focuses on new receiver design with low-resolution analog-to-digital converters (ADC). In future massive multiple-input-multiple-output (MIMO) systems, multiple high-speed high-resolution ADCs will become a bottleneck for practical applications because of the hardware complexity and power consumption. One solution to this problem is to adopt low-cost low-precision ADCs instead. In Chapter II, MU-MIMO-OFDM systems only equipped with low-precision ADCs are considered. A new turbo receiver structure is proposed to improve the overall system performance. Meanwhile, ultra-low-cost communication devices can enable massive deployment of disposable wireless relays. In Chapter III, the feasibility of using a one-bit relay cluster to help a power-constrained transmitter for distant communication is investigated. Nonlinear estimators are applied to enable effective decoding. The second topic focuses prototyping and verification of a LTE and WiFi co-existence system, where the operation of LTE in unlicensed spectrum (LTE-U) is discussed. LTE-U extends the benefits of LTE and LTE Advanced to unlicensed spectrum, enabling mobile operators to offload data traffic onto unlicensed frequencies more efficiently and effectively. With LTE-U, operators can offer consumers a more robust and seamless mobile broadband experience with better coverage and higher download speeds. As the coexistence leads to considerable performance instability of both LTE and WiFi transmissions, the LTE and WiFi receivers with MIMO interference canceller are designed and prototyped to support the coexistence in Chapter IV. The third topic focuses on theoretical analysis of physical-layer secrecy with finite blocklength. Unlike upper layer security approaches, the physical-layer communication security can guarantee information-theoretic secrecy. Current studies on the physical-layer secrecy are all based on infinite blocklength. Nevertheless, these asymptotic studies are unrealistic and the finite blocklength effect is crucial for practical secrecy communication. In Chapter V, a practical analysis of secure lattice codes is provided.
Actions of endothelin on isolated corpus cavernosum from rabbit and man (1990)

The effects of endothelin, a vasoconstrictor peptide produced by vascular endothelial cells, were investigated in isolated rabbit and human corpus cavernosum (CC). Preparations from both rabbit and man were potently contracted by endothelin in a concentration-dependent manner. The contractions developed slowly, could not be reversed despite frequent washings, and were only partly inhibited by the Ca2+ channel blocker nimodipine. Even in Ca2(+)-free medium containing the chelator EGTA a small contractile component persisted. In rabbit CC, the contractions in Ca2(+)-free medium were not affected by nimodipine, the Ca2(-)-channel agonist BAY K 86(44), or by depletion of intracellular Ca2(+) stores sensitive to noradrenaline (NA) and caffeine, but were almost abolished by the protein kinase C inhibitor H7. In both rabbit and man, carbachol and vasoactive intestinal polypeptide concentration-dependently relaxed preparations contracted by endothelin. The relaxations induced by carbachol were antagonized by atropine. Endothelin enhanced concentration-dependently the contractions induced by exogenously applied NA in rabbit CC. The enhancement was more pronounced at low concentrations of NA. This study shows that endothelin potently contracts isolated penile erectile tissue. The contraction seems to be mediated mainly by influx of Ca2+ through the cell membrane, which partly occurs through a pathway other than voltage-operated calcium channels. However, involvement of other mechanisms cannot be excluded. The results suggest that endothelin can play a role in penile erectile mechanisms.
Underwater image transmission and blurred image restoration (2001)

Because light is scattered and absorbed by seawater, the images obtained underwater are always blurry after transmitted through a certain distance. The point spread function (PSF) and modulation transfer function (MTF) are important properties of seawater to predict underwater light propagation and underwater image quality. We describe the laboratory facilities to measure the PSF and MTF of water body by the image transmission method and the restoration methods of under- water blurred images. The measured results are given and compared with the theories. The blurred images of a bar target transmitted through different water paths are restored with the measured MTF using a Wiener filter. © 2001 Society of Photo-Optical Instrumentation Engineers.
Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses (2009)

The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remains no clear explanation for these colony losses, with parasitic mites, viruses, bacteria, and fungal diseases all being proposed as possible candidates. Common characteristics that most failing colonies share is a lack of overt disease symptoms and the disappearance of workers from what appears to be normally functioning colonies. In this study, we used quantitative PCR to monitor the presence of three honeybee viruses, deformed wing virus (DWV), acute bee paralysis virus (ABPV), and black queen cell virus (BQCV), during a 1-year period in 15 asymptomatic, varroa mite-positive honeybee colonies in Southern England, and 3 asymptomatic colonies confirmed to be varroa mite free. All colonies with varroa mites underwent control treatments to ensure that mite populations remained low throughout the study. Despite this, multiple virus infections were detected, yet a significant correlation was observed only between DWV viral load and overwintering colony losses. The long-held view has been that DWV is relatively harmless to the overall health status of honeybee colonies unless it is in association with severe varroa mite infestations. Our findings suggest that DWV can potentially act independently of varroa mites to bring about colony losses. Therefore, DWV may be a major factor in overwintering colony losses.
How to share a secret (1979)

In this paper we show how to divide data D into n pieces in such a way that D is easily reconstructable from any k pieces, but even complete knowledge of k - 1 pieces reveals absolutely no information about D . This technique enables the construction of robust key management schemes for cryptographic systems that can function securely and reliably even when misfortunes destroy half the pieces and security breaches expose all but one of the remaining pieces.
Role of veiled cells in lymphocyte activation (1982)

Peripheral blood lymphocytes from normal rabbits or from rabbits hyperimmunized with human immunoglobulin were cultured in 20-microliters hanging droplets. The cultures were stimulated with concanavalin A or, in the case of cells from sensitized animals, with human immunoglobulin. The addition to the cultures of small numbers of autologous or allogeneic veiled cells separated from afferent lymph increased the responses to low doses of the stimulants, particularly when the cells were cultured at low cell densities or for short periods of time. At high cell densities or at longer periods of culture, stimulation occurred in the absence of added veiled cells and was associated with clumping of the lymphoid cells and development of cells which morphologically resembled veiled cells found in afferent lymph. The enhanced responses upon addition of small numbers of veiled cells were also correlated with the formation of lymphoid aggregates which were frequently held together by the elongated processes of a single veiled cell. The observations support the view that the veiled cells are lymph-borne precursors of dendritic cells in the lymph nodes. This may provide an in vitro model for the cellular relationships which occur in paracortical cords of lymph nodes following antigenic stimulation.
Structural mimicry in the phage [phis]21 N peptide–boxB RNA complex (2003)

We determined the solution structure of a 22-amino-acid peptide from the amino-terminal domain of the bacteriophage phi21 N protein in complex with its cognate 24-mer boxB RNA hairpin using heteronuclear magnetic resonance spectroscopy. The N peptide binds as an alpha-helix and interacts predominately with the major groove side of the 5' half of the boxB RNA stem-loop. This binding interface is defined by surface complementarity of polar and nonpolar interactions, and little sequence-specific recognition. The phi21 boxB loop (CUAACC) has hydrogen bond and backbone torsions typical of the "U-turn" motif, as well as base stacking of the last 4 nt, and a hydrogen bonded C:C pair closing the loop. The exposed face of the phi21 boxB loop, in complex with the N peptide, is strikingly similar to the GNRA tetraloop-like folds of the related lambda and P22 bacteriophage N peptide-boxB RNA complexes. The N peptide-boxB complexes of the various phage, while individually distinct, provide similar structural features for interactions with the Escherichia coli host factors to enable antitermination.
Autocorrelation pulse distortion in optical fiber CDMA systems employing ladder networks (1999)

This paper extends a pulse propagation previously developed by Marcuse for the characterization of pulse transmission in single mode fibers to the case of pulsed optical code-division multiple-access (CDMA) systems that employ encoders and decoders based on ladder networks. The model accounts for important degradation factors such as fiber dispersion, source linewidth (coherence), chip time duration and phase and delay mismatch between the source encoder and the destination decoder. Although results are only presented for the autocorrelation signal, the model can be employed to analyze the impact of the above mentioned.
New Antiepileptic Drugs Currently in Clinical Trials: Is There a Strategy in Their Development? (2002)

In designing and developing antiepileptic drugs (AEDs), attention should be paid to the desirable pharmacokinetic properties of potential new agents so that molecules are designed to achieve the desired pharmacodynamic and pharmacokinetic profiles. A review of current compounds in development or in clinical trials shows that several promising agents have incorporated pharmacokinetic-based design into their development process. This is particularly true for new AEDs that are second-generation or follow-up compounds of existing AEDs.
The glucuronoxylomannan of Cryptococcus neoformans serotype A is a type 2 T-cell-independent antigen (1992)

The humoral immune response of inbred mice to immunization with the glucuronoxylomannan (GXM) of Cryptococcus neoformans was investigated both serologically and in plaque-forming cells (PFCs). The T-helper-cell-independent quality of the GXM was demonstrated by using BALB/c nu/nu mice. Primary and secondary dose responses to three antigenic forms of GXM, (i) the native antigen, (ii) a GXM-bovine serum albumin protein conjugate, and (iii) a cryptococcal whole-cell vaccine, revealed a lack of isotype class switching and anamnestic responses. Both the levels of complement-fixing anti-GXM antibody in serum and the PFC responses in the athymic mice showed no significant differences from those in the wild-type controls. However, T cells are involved in the suppression of the primary response to GXM. When BALB/cBy mice were given rabbit anti-mouse thymocyte serum along with 0.5 microgram of GXM, both antibody levels in serum and PFC responses were significantly increased over those of control mice that received GXM and normal rabbit serum. In addition, T cells were also shown to enhance the primary immune response to GXM. BALB/cBy mice were given GXM and anti-mouse thymocyte serum on day 1. On day 2, the experimental group was given anti-mouse thymocyte serum and the control group was given saline. On day 5, comparison of the PFC responses and anti-GXM antibody titers of the two groups revealed a significant increase in the immune response of the control over the experimental group. The type 2 T-cell-independent quality of GXM was also demonstrated in CBA/cHN xid mice. These mice lack the Lyb+ subset of B cells and are unable to respond to type 2 T-independent antigens but respond normally to type 1 T-independent antigens. Type III pneumococcal polysaccharide, a type 2 T-independent antigen, was used as a negative control, and trinitrophenyl-lipopolysaccharide, a type 1 T-independent antigen, was used as a positive control. The CBA/cHN xid mice failed to respond to either type III pneumococcal polysaccharide or GXM but did not respond to immunization with trinitrophenyl-lipopolysaccharide. BALB/cBy mice responded normally to all three antigens.
Long-term trends in cancer mortality in the United States, 1930-1998 (2003)

The trends of decreasing cancer death rates for the leading cancer sites in the 1990s are encouraging. However, surveillance researchers must continue to monitor these declines to assess whether the progress seen in this decade persists. Efforts also must be made to study the sites with increasing trends and identify potential underlying causes.
Human neural stem cells improve cognitive function of aged brain (2001)

The capability for in vitro expansion of human neural stem cells (HNSCs) provides a well characterized and unlimited source alternative to using primary fetal tissue for neuronal replacement therapies. The HNSCs, injected into the lateral ventricle of 24-month-old rats after in vitro expansion, displayed extensive and positional incorporation into the aged host brain with improvement of cognitive score assessed by the Morris water maze after 4 weeks of the transplantation. Our results demonstrate that the aged brain is capable of providing the necessary environment for HNSCs to retain their pluripotent status and suggest the potential for neuroreplacement therapies in age-associated neurodegenerative disease.
Coded-aperture DUV spectrometer for stand-off Raman spectroscopy (2009)

We have designed and constructed a coded aperture spectrometer for use in the deep UV range. The Czerny-Turner design provides sufficient spectral resolution to observe Raman scattering features, while the use of a coded aperture provides a greatly improved light collection efficiency for scattering sources. The resulting instrument is capable of analyzing Raman spectra from samples at a 1 meter viewing distance. We provide an overview of the system, its optical design, and some preliminary measurements.
NY-ESO-1 Based Immunotherapy of Cancer: Current Perspectives (2018)

NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy. In this review, we provide background information on NY-ESO-1 expression and function in normal and cancerous tissues. Furthermore, NY-ESO-1-specific immune responses have been observed in various cancer types; however, their utility as biomarkers are not well determined. Finally, we describe the immune-based therapeutic options targeting NY-ESO-1 that are currently in clinical trial. We will highlight the recent advancements made in NY-ESO-1 cancer vaccines, adoptive T cell therapy, and combinatorial treatment with checkpoint inhibitors and will discuss the current trends for future NY-ESO-1 based immunotherapy. Cancer treatment has been revolutionized over the last few decades with immunotherapy emerging at the forefront. Immune-based interventions have shown promising results, providing a new treatment avenue for durable clinical responses in various cancer types. The majority of successful immunotherapy studies have been reported in liquid cancers, whereas these approaches have met many challenges in solid cancers. Effective immunotherapy in solid cancers is hampered by the complex, dynamic tumor microenvironment that modulates the extent and phenotype of the antitumor immune response. Furthermore, many solid tumor-associated antigens are not private but can be found in normal somatic tissues, resulting in minor to detrimental off-target toxicities. Therefore, there is an ongoing effort to identify tumor-specific antigens to target using various immune-based modalities. CTAs are considered good candidate targets for immunotherapy as they are characterized by a restricted expression in normal somatic tissues concomitant with a re-expression in solid epithelial cancers. Moreover, several CTAs have been found to induce a spontaneous immune response, NY-ESO-1 being the most immunogenic among the family members. Hence, this review will focus on NY-ESO-1 and discuss the past and current NY-ESO-1 targeted immunotherapeutic strategies.