gebhart/patrepeval huggingface dataset:
Host cell heparan sulfate proteoglycans mediate attachment and entry of Listeria monocytogenes, and the listerial surface protein ActA is involved in heparan sulfate receptor recognition (1997)

The mechanisms by which the intracellular pathogen Listeria monocytogenes interacts with the host cell surface remain largely unknown. In this study, we investigated the role of heparan sulfate proteoglycans (HSPG) in listerial infection. Pretreatment of bacteria with heparin or heparan sulfate (HS), but not with other glycosaminoglycans, inhibited attachment and subsequent uptake by IC-21 murine macrophages and CHO epithelial-like cells. Specific removal of HS from target cells with heparinase III significantly impaired listerial adhesion and invasion. Mutant CHO cells deficient in HS synthesis bound and internalized significantly fewer bacteria than wild-type cells did. Pretreatment of target cells with the HS-binding proteins fibronectin and platelet factor 4, or with heparinase III, impaired listerial infectivity only in those cells expressing HS. Moreover, a synthetic peptide corresponding to the HS-binding ligand in Plasmodium falciparum circumsporozoite protein (pepPf1) inhibited listerial attachment to IC-21 and CHO cells. A motif very similar to the HS-binding site of pepPf1 was found in the N-terminal region of ActA, the L. monocytogenes surface protein responsible for actin-based bacterial motility and cell-to-cell spread. In the same region of ActA, several clusters of positively charged amino acids which could function as HS-binding domains were identified. An ActA-deficient mutant was significantly impaired in attachment and entry due to altered HS recognition functions. This work shows that specific interaction with an HSPG receptor present on the surface of both professional and nonprofessional phagocytes is involved in L. monocytogenes cytoadhesion and invasion and strongly suggests that the bacterial surface protein ActA may be a ligand mediating HSPG receptor recognition.
Mantle Cell Lymphoma A Proposal for Unification of Morphologic, Immunologic, and Molecular Data (1992)

Banks, P. M. M.D.; Chan, J. M.D.; Cleary, M. L. M.D.; Delsol, G. M.D.; De Wolf-Peeters, C. M.D.; Gatter, K. M.D.; Grogan, T. M. M.D.; Harris, N. L. M.D.; Isaacson, P. G. M.D.; Jaffe, E. S. M.D.; Mason, D. M.D.; Pileri, S. M.D.; Ralfkiaer, E. M.D.; Stein, H. M.D.; Warnke, R. A. M.D. Author Information
Internet Privacy: At Home and At Work (2001)

This paper is a tutorial on Internet privacy. The objective of the tutorial is to inform users of the Internet how and why their use of the Internet can be monitored. The focus of the tutorial is Internet privacy at home and in the work place. The tutorial contains three major sections: Technical Background, Privacy At Home and Privacy At Work. The At Home section is concerned with personal privacy infringement. This section details who would be interested in personal privacy infringement, for what purpose, and how they would accomplish it. The At Home section also discusses techniques on how to avoid privacy infringement. The At Work section is concerned with employee surveillance. Topics discussed include who would be interested in this surveillance, for what purpose, and how this surveillance would be carried out. The At Work section also discusses the concept of an Acceptable Use Policy (AUP) Both of these sections require a bit of technical expertise to understand how Internet activity can be monitored. The Technical Background section and the Appendix review the client/server paradigm of web computing, the important details of the Hypertext Transfer Protocol (HTTP), and presents an overview of Uniform Resource Identifiers (URIs). These concepts are necessary to understand how Internet activity can be monitored.
A new scattering method that combines roughness and diffraction effects (2005)

Most of today’s room acoustics programs make use of scattering coefficients which are used in order to describe surface scattering (roughness of material) and scattering of reflected sound caused by limited surface size (diffraction). A method which combines scattering caused by diffraction due to typical surface dimension, angle of incidence, and incident path length with surface scattering is presented. Each of the two scattering effects is modeled as frequency dependent functions. The benefits are two-fold: (a) Separating the user specified surface scattering coefficient from the room geometry, makes it easier for the user to make good guesstimates of the coefficients which will be in better agreement with the ones which can be measured. In many cases a scattering coefficient of say 1% for all surfaces may be sufficient. (b) Scattering due to diffraction is distance and angle dependent and as such it is not known before the actual raytracing or image source detection takes place. An example of this is that a desktop will provide a strong specular component to its user whereas it will provide scattered sound at far distances.
Presence of Haemagglutinin in the Envelope of Extracellular Vaccinia Virus Particles (1976)

The relationship of vaccinia haemagglutinin (HA) to extracellular enveloped virus (EEV) was examined. EEV banded in caesium chloride gradients at a density of 1.23 to 1.24 g/ml coincident with a peak of HA activity. EEV of an HA+ vaccinia strain showed greater than 90% adsorption to rooster red blood cells (RBCs) as detected by infectivity and 3H-thymidine labelling whereas intracellular naked virus (INV) of the HA+ strain and EEV of an HA- strain failed to show significant adsorption. The adsorption was specifically inhibited by antiserum to vaccinia. Adsorption kinetic experiments demonstrated a lack of temperature dependence on the total amount of EEV adsorbed. No elution of EEV from RBCs could be detected. The capacity of EEV to adsorb to RBCs was found to be stable at 56 degrees C for 30 min.
Greater osteoblast and endothelial cell adhesion on nanostructured polyethylene and titanium (2010)

Mostly due to desirable mechanical properties (such as high durability and low wear), certain synthetic polymers (such as polyethylene) and metals (such as titanium) have found numerous applications in the medical device arena from orthopedics to the vasculature, yet frequently, they do not proactively encourage desirable cell responses. In an effort to improve the efficacy of such traditional materials for various implant applications, this study used electron beam evaporation to create nanostructured surface features that mimic those of natural tissue on polyethylene and titanium. For other materials, it has been shown that the creation of nanorough surfaces increases surface energy leading to greater select protein (such as vitronectin and fibronectin) interactions to increase specific cell adhesion. Here, osteoblast (bone forming cells) and endothelial cell (cells that line the vasculature) adhesion was determined on nanostructured compared to conventional, nano-smooth polyethylene and titanium. Results demonstrated that nanorough surfaces created by electron beam evaporation increased the adhesion of both cells markedly better than conventional smooth surfaces. In summary, this study provided evidence that electron beam evaporation can modify implant surfaces (specifically, polyethylene and titanium) to have nanostructured surface features to improve osteoblast and endothelial cell adhesion. Since the adhesion of anchorage dependent cells (such as osteoblasts and endothelial cells) is a prerequisite for their long-term functions, this study suggests that electron beam evaporation should be further studied for improving materials for various biomedical applications.
Serum cystatin C is independently associated with renal impairment and high sensitivity C-reactive protein in systemic lupus erythematosus. (2013)

In SLE, sCysC may be influenced by low grade inﬂammation as well as by renal dysfunction. Therefore, SCysC should not supplant current assessment of renal dysfunction in SLE.
A High Affinity Hepatocyte Growth Factor-binding Site in the Immunoglobulin-like Region of Met (2008)

Hepatocyte growth factor (HGF) and its high affinity receptor, the tyrosine kinase Met, play a key role in embryo development and tumor invasion. Both HGF and Met are established targets for cancer therapy. However, the mechanism of their interaction is complex and remains elusive. HGF is secreted as a monomeric precursor (pro-HGF) that binds to but does not activate Met. Mature HGF is a alpha/beta heterodimer containing a high affinity Met-binding site in the alpha-chain (HGF-alpha) and a low affinity Met-binding site in the beta-chain (HGF-beta). The extracellular portion of Met contains a semaphorin (Sema) domain, a cysteine-rich hinge (plexin-semaphorin-integrin), and four immunoglobulin-like domains (immunoglobulin-like regions in plexins and transcription factors (IPT) 1-4). HGF-beta binds to Sema through a low affinity contact. The domain of Met responsible for high affinity binding to HGF-alpha has not been identified yet. Here we show that this long sought after binding site lies in the immunoglobulin-like region of Met and more precisely in IPT 3 and 4. We also show that IPT 3 and 4 are sufficient to transmit the signal for kinase activation to the cytoplasm, although the lack of Sema makes the receptor equally sensitive to mature HGF and pro-HGF. Finally, we provide evidence that soluble Met-derived proteins containing either the low affinity or high affinity HGF-binding site antagonize HGF-induced invasive growth both in vitro and in xenografts. These data suggest that the immunoglobulin-like region of Met cooperates with the Sema domain in binding to HGF and in controlling Met kinase activity. Although the IPT-HGF-alpha interaction provides binding strength, the Sema-HGF-beta contact confers selective sensitivity to the active form of the ligand.
PEMBENTUKAN KONSEP DIRI ANAK USIA DINI DI ONE EARTH SCHOOL BALI (2012)

We were not born with clear understandings of who we are and what our value is. Communication with others can help us to understand and know who we are. For children at their early age, they are not yet to comprehend who they are, therefore they need someone to help them know who they are through particular others. This paper describes the process of how self-concept is formed during golden age (0-8 yo) through interpersonal communication between teacher and kindergarten students. The method in this research is case study at One Earth School Bali with qualitative approach using in-depth interview towards several informants purposively. The results showed that the understanding of the age factor as a volatile mood and playful nature of children, then the method of playing while learning in education as well as interpersonal communication teachers and children to build good relationships were potentially form positive self-concept of children, so children have good self-esteem and self-image.
Diagnosis of Cerebral Amyloid Angiopathy (1997)

These data help to define criteria for the diagnosis of CAA-related hemorrhage from surgical specimens.
Truncated APC regulates the transcriptional activity of β-catenin in a cell cycle dependent manner (2006)

Most colon cancer cells express truncated versions of the tumour suppressor Adenomatous Polyposis Coli (APC). These molecules are selected during tumourigenesis for impaired beta-catenin degrading activity. In this study, we describe that truncated APC can still control the activity of beta-catenin in colon cancer cell lines via its first 20 amino acid repeat. First, we show that both endogenous and ectopically expressed truncated APC molecules can bind to beta-catenin. Second, reduction of the levels of truncated APC by RNA interference increases the activity of a beta-catenin-dependent reporter gene and stimulates the expression of the beta-catenin target gene AXIN2/conductin. This occurs without alterations of the amounts of cytosolic beta-catenin. Conversely, ectopic expression of truncated APC decreases beta-catenin-dependent transcription without affecting the intensity of immunofluorescence staining of beta-catenin in transfected cells. Third, we reveal that the APC level increases when cells reach the G1-S boundary during cell cycle progression. Simultaneously, the amount of beta-catenin bound to APC increases and the transcriptional activity of beta-catenin drops in an APC-dependent manner. Again, this occurs independently of the amounts of either total or phosphorylated cytosolic beta-catenin. Together, these results indicate that truncated APC controls the ability of beta-catenin to activate transcription. As we also show that the inhibition involves the first 20 amino acid repeat of APC, our data suggest that colon cancer cells retain a truncated APC molecule containing at least the first 20 amino acid repeat to modulate the transcriptional activity of beta-catenin in a cell cycle-dependent manner.
Strategies for Coupling Molecular Units if Subsequent Decoupling Is Required (2012)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTStrategies for Coupling Molecular Units if Subsequent Decoupling Is RequiredRoman Bielski*† and Zbigniew Witczak*‡View Author Information† Value Recovery, Inc., 510 Heron Drive, Suite 301, Bridgeport, New Jersey 08014, United States‡ Department of Pharmaceutical Sciences, Nesbitt School of Pharmacy, Wilkes University, Wilkes-Barre, Pennsylvania 18766, United States*E-mail addresses: R.B., [email protected]; Z.W., [email protected]Cite this: Chem. Rev. 2013, 113, 3, 2205–2243Publication Date (Web):November 15, 2012Publication History Received26 August 2011Published online15 November 2012Published inissue 13 March 2013https://doi.org/10.1021/cr200338qCopyright © 2012 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views3043Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (8 MB) Get e-AlertsSUBJECTS:Crystal cleavage,Molecules,Organic compounds,Peptides and proteins,Pharmaceuticals Get e-Alerts
Biochemical Function and Ecological Significance of Novel Bacterial Lipids in Deep-Sea Procaryotes (1986)

The fatty acid composition of the membrane lipids in 11 deep-sea bacterial isolates was determined. The fatty acids observed were typical of marine vibrios except for the presence of large amounts of long-chain polyunsaturated fatty acids (PUFAs). These long-chain PUFAs were previously thought to be absent in procaryotes, with the notable exception of a single marine Flexibacter sp. In three barophilic strains tested at 2 degrees C, there was a general increase in the relative amount of PUFAs as pressure was increased from a low growth pressure towards the optimal growth pressure. In Vibrio marinus MP-1, a psychrophilic strain, PUFAs were found to increase as a function of decreasing temperature at constant atmospheric pressure. These results suggest the involvement of PUFAs in the maintenance of optimal membrane fluidity and function over environmentally relevant temperatures and pressures. Furthermore, since these lipids are essential nutrients for higher taxa and are found in large amounts in the lipids of deep-sea vertebrates and invertebrates, an important, specific role for deep-sea bacteria in abyssal food webs is implicated.
Reduced Susceptibility of Recombinant Polyclonal Antibodies to Inhibitory Anti-Variable Domain Antibody Responses (2006)

The immunogenicity of therapeutic Abs is a concern as anti-drug Abs may impact negatively on the pharmacodynamics and safety profile of Ab drugs. The factors governing induction of anti-drug Abs are not fully understood. In this study, we describe a model based on mouse-human chimeric Abs for the study of Ab immunogenicity in vivo. Six chimeric Abs containing human V regions and mouse C regions were generated from six human anti-Rhesus D Abs and the Ag-binding characteristics of the parental human Abs were retained. Analysis of the immune response toward the individual chimeric Abs revealed the induction of anti-variable domain Abs including anti-idiotypic Abs against some of these, thereby demonstrating the applicability of the model for studying anti-drug Ab responses in vivo. Immunization of BALB/c, C57, and outbred NMRI mice with a polyclonal composition consisting of all six chimeric Abs demonstrated that the immunogenicity of the individual Abs was haplotype dependent. Chimeric Abs, which were nonimmunogenic when administered individually, did not become immunogenic as part of the polyclonal composition, implying the absence of epitope spreading. Ex vivo Ab-binding studies established a clear correlation between the level of immunogenicity of the Abs comprised in the composition and the impact on the pharmacology of the Abs. These analyses demonstrate that under these conditions this polyclonal Ab composition was generally less susceptible to blocking Abs than the respective mAbs.
Polymer-Based Tris(2-phenylpyridine)iridium Complexes (2006)

A series of fac-Ir(ppy)3 complex functionalized poly(styrene)s and poly(N-vinylcarbazole)-co-poly(styrene)s were synthesized. The emission wavelength of all polymers could be readily tuned from 550 to 515 nm by reducing the linkage of the Ir complex to the polymer backbones from a Schiff base to an amine. The reduced polymers retained the photophysical properties of the corresponding small molecule fac-Ir(ppy)3, including transient absorption behaviors, high emission quantum yields (0.12−0.23), and long excited-state lifetimes (963−1221 ns). These results clearly indicate that the polymer backbones do not interfere with the photophysical properties of the pendant fac-Ir(ppy)3 chromophore but endow solution processability to these materials for potential use in light-emitting devices and emissive display technology.
Cystic fibrosis: A worldwide analysis ofCFTR mutations?correlation with incidence data and application to screening (2002)

Although there have been numerous reports from around the world of mutations in the gene of chromosome 7 known as CFTR (cystic fibrosis transmembrane conductance regulator), little attention has been given to integrating these mutant alleles into a global understanding of the population molecular genetics associated with cystic fibrosis (CF). We determined the distribution of CFTR mutations in as many regions throughout the world as possible in an effort designed to: 1) increase our understanding of ancestry-genotype relationships, 2) compare mutational arrays with disease incidence, and 3) gain insight for decisions regarding screening program enhancement through CFTR multi-mutational analyses. Information on all mutations that have been published since the identification and cloning of the CFTR gene's most common allele, DeltaF508 (or F508del), was reviewed and integrated into a centralized database. The data were then sorted and regional CFTR arrays were determined using mutations that appeared in a given region with a frequency of 0.5% or greater. Final analyses were based on 72,431 CF chromosomes, using data compiled from over 100 original papers, and over 80 regions from around the world, including all nations where CF has been studied using analytical molecular genetics. Initial results confirmed wide mutational heterogeneity throughout the world; however, characterization of the most common mutations across most populations was possible. We also examined CF incidence, DeltaF508 frequency, and regional mutational heterogeneity in a subset of populations. Data for these analyses were filtered for reliability and methodological strength before being incorporated into the final analysis. Statistical assessment of these variables revealed that there is a significant positive correlation between DeltaF508 frequency and the CF incidence levels of regional populations. Regional analyses were also performed to search for trends in the distribution of CFTR mutations across migrant and related populations; this led to clarification of ancestry-genotype patterns that can be used to design CFTR multi-mutation panels for CF screening programs. From comprehensive assessment of these data, we offer recommendations that multiple CFTR alleles should eventually be included to increase the sensitivity of newborn screening programs employing two-tier testing with trypsinogen and DNA analysis.
Oxidative stress: The achilles' heel of neurodegenerative diseases of the retina (2011)

Age-related macular degeneration (AMD) is the leading cause of blindness among adults in the developed countries. It is characterized by the progressive loss of central vision. AMD is classified into two forms: dry and wet. Dry AMD involves the accumulation of deposits in the RPE and Bruch's membrane; Wet AMD is characterized by neovascularization in the choroid. Whether the two forms of AMD share the same mechanism for the disease development is presently not clear. Oxidative stress, inflammation, and ER-stress are the common modes for the pathogenesis of AMD. In addition, other risk factors and several signaling pathways have been implicated as causative factors of AMD. In this paper, the mechanisms underlying AMD, risk factors involved in the pathology, representative animal models, and therapeutic treatment strategies are reviewed.
A New Improved Biodegradable Tracheal Prosthesis Using Hydroxy Apatite and Carbon Fiber (1989)

A new biodegradable tracheal prosthesis was developed using hydroxyapatite rings as the artificial tracheal cartilage, a carbon fiber tube as the tracheal tube; it was then implanted into the cervical trachea in dogs. Morphologic examination revealed that the hydroxyapatite ring was anchored firmly to the tracheal cartilage by ingrowth of cartilaginous tissue into the macropores of the hydroxyapatite.
The Structure of Aggrecan Fragments in Human Synovial Fluid (1992)

Synovial fluid was collected from patients with recent knee injury and from patients with early or late stage osteoarthritis. Chondroitin sulfate-substituted aggrecan fragments present in these fluids, and in normal bovine synovial fluid, were purified by cesium chloride gradient centrifugation, enzymically deglycosylated and fractionated by gel filtration on Superose-12. Eachsample contained twomajoraggrecan core protein populations with apparent molecular masses of 90 kD and 150 kD. For all samples, NH2-terminal analysis of both populations gave a single major sequence beginning ARGSV. This NH2 terminus results from cleavage ofthehuman aggrecan core protein at the Glu 373-Ala 374 bond within the interglobular domain between theG1 andG2 domains. Cleavage at this site also occurs during control and interleukin-1 stimulated aggrecan ca