gebhart/patrepeval huggingface dataset:
Mitotic inactivation of a human SWI/SNF chromatin remodeling complex (1998)

During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and hBrm, which are components of human SWI/SNF (hSWI/SNF) complexes, were recently shown to be phosphorylated during mitosis. This suggested that phosphorylation might be used as a switch to modulate SWI/SNF activity. Using an epitope-tag strategy, we have purified hSWI/SNF complexes at different stages of the cell cycle, and found that hSWI/SNF was inactive in cells blocked in G2-M. Mitotic hSWI/SNF contained Brg1 but not hBrm, and was phosphorylated on at least two subunits, hSWI3 and Brg1. In vitro, active hSWI/SNF from asynchronous cells can be phosphorylated and inactivated by ERK1, and reactivated by dephosphorylation. hSWI/SNF isolated as cells traversed mitosis regained activity when its subunits were dephosphorylated either in vitro or in vivo. We propose that this transitional inactivation and reactivation of hSWI/SNF is required for formation of a repressed chromatin structure during mitosis and reformation of an active chromatin structure as cells leave mitosis.
Fiber Network Service Survivability (1992)

From the Publisher:
This book examines the architectures, technologies, and design methods that make it possible to achieve a survivable fiber network, both practically and economically. Combining theory and actual results, it explores alternative methods and presents analytical and computational optimization approaches.
Caged Protein Prenyltransferase Substrates: Tools for Understanding Protein Prenylation (2008)

Originally designed to block the prenylation of oncogenic Ras, inhibitors of protein farnesyltransferase currently in preclinical and clinical trials are showing efficacy in cancers with normal Ras. Blocking protein prenylation has also shown promise in the treatment of malaria, Chagas disease and progeria syndrome. A better understanding of the mechanism, targets and in vivo consequences of protein prenylation are needed to elucidate the mode of action of current PFTase (Protein Farnesyltransferase) inhibitors and to create more potent and selective compounds. Caged enzyme substrates are useful tools for understanding enzyme mechanism and biological function. Reported here is the synthesis and characterization of caged substrates of PFTase. The caged isoprenoid diphosphates are poor substrates prior to photolysis. The caged CAAX peptide is a true catalytically caged substrate of PFTase in that it is to not a substrate, yet is able to bind to the enzyme as established by inhibition studies and X-ray crystallography. Irradiation of the caged molecules with 350 nm light readily releases their cognate substrate and their photolysis products are benign. These properties highlight the utility of those analogs towards a variety of in vitro and in vivo applications.
C2-Streptavidin Mediates the Delivery of Biotin-Conjugated Tumor Suppressor Protein P53 into Tumor Cells (2013)

We have previously generated a recombinant C2-streptavidin fusion protein for the delivery of biotin-labeled molecules of low molecular weight into the cytosol of mammalian cells. A nontoxic moiety of Clostridium botulinum C2 toxin mediates the cellular uptake, whereas the streptavidin unit serves as a binding platform for biotin-labeled cargo molecules. In the present study, we used the C2-streptavidin transporter to introduce biotin-conjugated p53 protein into various mammalian cell lines. The p53 tumor suppressor protein is inactivated in many human cancers by multiple mechanisms and therefore the restoration of its activity in tumor cells is of great therapeutic interest. Recombinant p53 was expressed in insect cells and biotin-labeled. Biotin-p53 retained its specific high-affinity DNA-binding as revealed by gel-shift analysis. Successful conjugation of biotin-p53 to the C2-streptavidin transporter was monitored by an overlay blot technique and confirmed by real-time surface plasmon resonance, providing a KD-value in the low nM range. C2-streptavidin significantly enhanced the uptake of biotin-p53 into African Green Monkey (Vero) epithelial cells as shown by flow cytometry. Using cell fractionation, the cytosolic translocation of biotin-p53 was detected in Vero cells as well as in HeLa cervix carcinoma cells. In line with this finding, confocal microscopy displayed cytoplasmic staining of biotin-p53 in HeLa and HL60 leukemia cells. Internalized biotin-p53 partially colocalized with early endosomes, as confirmed by confocal microscopy. In conclusion, our results demonstrate the successful conjugation of biotin-p53 to C2-streptavidin and its subsequent receptor-mediated endocytosis into different human tumor cell lines.
Characterization of polarization attributes of seismic waves using continuous wavelet transforms (2006)

Abstract Complex-trace analysis is the method of choice for analyzing polarized data. Because particle motion can be represented by instantaneous attributes that show distinct features for waves of different polarization characteristics, it can be used to separate and characterize these waves. Traditional methods of complex-trace analysis only give the instantaneous attributes as a function of time or frequency. However, for transient wave types or seismic events that overlap in time, an estimate of the polarization parameters requires analysis of the time-frequency dependence of these attributes. We propose a method to map instantaneous polarization attributes of seismic signals in the wavelet domain and explicitly relate these attributes with the wavelet-transform coefficients of the analyzed signal. We compare our method with traditional complex-trace analysis using numerical examples. An advantage of our method is its possibility of performing the complete wave-mode separation/filtering process in the wavelet domain and its ability to provide the frequency dependence of ellipticity, which contains important information on the subsurface structure. Furthermore, using 2-C synthetic and real seismic shot gathers, we show how to use the method to separate different wave types and identify zones of interfering wave modes.
Significance of thromboxane generation in ozone-induced airway hyperresponsiveness in dogs (1985)

To determine whether thromboxane A2 may be involved in ozone (O3)-induced airway hyperresponsiveness, we studied the effect of a thromboxane synthase inhibitor (OKY-046, 100 micrograms X kg-1 X min-1 iv) in five dogs exposed to O3. Airway responsiveness was assessed by determining the provocative concentration of acetylcholine aerosol that increased total pulmonary resistance by 5 cmH2O X l-1 X s. O3 (3 ppm) increased airway responsiveness as demonstrated by a decrease in acetylcholine provocative concentration from 2.42 (geometric SEM = 1.64) to 0.14 mg/ml (geometric SEM = 1.30). OKY-046 significantly inhibited this effect without altering pre-O3 responsiveness or the O3-induced increase in neutrophils and airway epithelial cells in bronchoalveolar lavage fluid. To further examine the role of thromboxane A2, we studied the effect of a thromboxane A2 mimetic, U-46619, on airway responsiveness in five additional dogs. U-46619 in subthreshold doses (i.e., insufficient to increase base-line pulmonary resistance) caused a fourfold increase in airway responsiveness to acetylcholine. Subthreshold doses of histamine had no effect. These results suggest that thromboxane A2 may be an important mediator of O3-induced airway hyperresponsiveness.
Computational Single-shot Hyper-spectral Imaging based on a Microstructured Diffractive Optic (2016)

A compact hyper-spectral imaging system using a microstructured transparent diffractive optic is experimentally demonstrated with enhanced spatial resolution. A fast algorithm based on regularization is exploited to reconstruct hyper-spectral data in a single shot.
Ang-2-VEGF-A CrossMab, a Novel Bispecific Human IgG1 Antibody Blocking VEGF-A and Ang-2 Functions Simultaneously, Mediates Potent Antitumor, Antiangiogenic, and Antimetastatic Efficacy (2013)

These data establish Ang-2-VEGF-A CrossMab as a promising antitumor, antiangiogenic, and antimetastatic agent for the treatment of cancer.
A tumor-associated antigen specific for human kappa myeloma cells. (1983)

A monoclonal antibody (K-1-21) raised against a kappa Bence Jones protein exhibits unique binding properties to malignant plasma cells. K-1-21 is an IgG1 kappa antibody that reacts with human kappa light chains in free form, but shows no reactivity with heavy chain-associated kappa light chains. By immunofluorescence, K-1-21 binds to the surface of LICR LON/HMy2 (HMy2) kappa myeloma cells and to plasma cells from a majority (8/11) of patients with various types of kappa myeloma; it did not bind to the surface of normal cells, nor to malignant cells of non-kappa myeloma origin. Flow cytometry analysis of K-1-21 binding to HMy2 cells indicated that the surface reactivity of K-1-21 could be completely inhibited by preincubation of the antibody with purified kappa light chains, whereas no inhibition occurred after preincubation with lambda chains or intact human IgG. Thus, the epitope recognized by K-1-21 on the cell surface may be similar, if not identical, to the determinant recognized on soluble free kappa light chains, and constitutes a tumor-associated antigen with selectivity for kappa myeloma cells. K-1-21 may therefore have clinical potential in patients with kappa myeloma.
Preparation of hydrazino-modified proteins and their use for the synthesis of technetium-99m-protein conjugates (1991)

The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired 99mTc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.
Domain based Internet security policy management (2002)

As security devices and protocols become widely used on the Internet, the task of managing and processing communication security policies grows steeply in its complexity. This paper presents a scaleable, robust, secure distributed system that can manage communication security policies associated with multiple network domains and resolving the policies-esp. those that specify the use of IP-AH/ESP security protocols-into security requirements for inter-domain communication. Technology innovation includes a formal model for IPsec policy specification and resolution, a platform independent policy specification language and a distributed policy server system. The formal model consists of a hierarchical domain model for IPsec policy enforcement and a lattice model of IPsec policy semantics. The policy specification language enables users to specify IPsec policies using the formal model regardless of the make of the security devices. The policy servers maintain the security policies in a distributed database, and negotiate the security associations for protecting inter-domain communication. Both the policy database and the policy exchange protocol are protected from passive and active attacks. Several UNIX implementations are available for non-commercial uses.
Control of X-Ray Beam Fluctuation in Synchrotron Radiation Lithography Beamline (1995)

Using the focusing property of beamline optics, we have presented a new technique to directly measure the position and emission angle drifts of the synchrotron radiation (SR) source which may deteriorate the optical performance of a beamline consisting of focusing mirrors. A new methodology is also proposed to correct the SR source drifts, combining the above measuring technique, a SR beam position monitor (SRBPM) mounted on the beamline and the excitation of two steering magnets installed in a storage ring.
Toxicity Induced by Subchronic Dermal Exposure to Paratertiary Butyl Benzoic Acid (pt BBA) in Fischer 344 Rats (1989)

The subchronic dermal toxicity of aqueous solutions containing the diethanolamine salt of para-tertiary butyl benzoic acid was determined in Fischer 344 rats. Para-tertiary butyl benzoic acid (pt BBA) has important applications in the manufacture of resins, polymers, and corrosion inhibitors. Male and female rats were exposed topically 5 days/week for up to 13 weeks with dosing solutions that resulted in daily exposures of 0.0, 17.5, 35.0, 70.0, or 140.0 mg/kg pt BBA. These concentrations did not produce overt clinical signs of toxicity and did not cause irritation to dermal exposure sites. However, exposure to the two highest concentrations resulted in decreased weight gain. Exposure at all concentrations resulted in dosage-related increases in relative hepatic and renal weights. Exposure of males to the two highest concentrations caused decreased testis weight. Exposure-related pathologic changes were confined to three organ systems: cytoplasmic vacuolation in the liver; tubular dilation and papillary necrosis of the kidneys; and tubular degeneration in the testes. Accompanying aberations in clinical chemistry values suggested altered hepatic and renal function. In males exposed daily to 70.0 and 140.0 mg/kg pt BBA, the testicular effects were marked, but no effects were detected in rats exposed to 17.5 or 35.0 mg/kg of pt BBA.
Minimal Residual Disease Assessed by Multiparameter Flow Cytometry in Multiple Myeloma: Impact on Outcome in the Medical Research Council Myeloma IX Study (2013)

MRD assessment by MFC was predictive of overall outcome in patients with myeloma undergoing ASCT. This predictive value was seen in patients achieving conventional CR as well as patients with favorable and adverse cytogenetics. The effects of maintenance strategies can also be evaluated, and our data suggest that maintenance thalidomide can eradicate MRD in some patients.
Hepatitis B virus nuclear export elements: RNA stem-loop α and β, key parts of the HBV post-transcriptional regulatory element (2016)

Many viruses contain RNA elements that modulate splicing and/or promote nuclear export of their RNAs. The RNAs of the major human pathogen, hepatitis B virus (HBV) contain a large (~600 bases) composite cis-acting 'post-transcriptional regulatory element' (PRE). This element promotes expression from these naturally intronless transcripts. Indeed, the related woodchuck hepadnavirus PRE (WPRE) is used to enhance expression in gene therapy and other expression vectors. These PRE are likely to act through a combination of mechanisms, including promotion of RNA nuclear export. Functional components of both the HBV PRE and WPRE are 2 conserved RNA cis-acting stem-loop (SL) structures, SLα and SLβ. They are within the coding regions of polymerase (P) gene, and both P and X genes, respectively. Based on previous studies using mutagenesis and/or nuclear magnetic resonance (NMR), here we propose 2 covariance models for SLα and SLβ. The model for the 30-nucleotide SLα contains a G-bulge and a CNGG(U) apical loop of which the first and the fourth loop residues form a CG pair and the fifth loop residue is bulged out, as observed in the NMR structure. The model for the 23-nucleotide SLβ contains a 7-base-pair stem and a 9-nucleotide loop. Comparison of the models with other RNA structural elements, as well as similarity searches of human transcriptome and viral genomes demonstrate that SLα and SLβ are specific to HBV transcripts. However, they are well conserved among the hepadnaviruses of non-human primates, the woodchuck and ground squirrel.
The 100-.ANG.-order depth profile control of polypyrrole-poly(3-methylthiophene) composite thin film by potential-programmed electropolymerization (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe 100-.ANG.-order depth profile control of polypyrrole-poly(3-methylthiophene) composite thin film by potential-programmed electropolymerizationTomokazu Iyoda, Hideo Toyoda, Mamoru Fujitsuka, Reiko Nakahara, Hajime Tsuchiya, Kenichi Honda, and Takeo ShimidzuCite this: J. Phys. Chem. 1991, 95, 13, 5215–5220Publication Date (Print):June 1, 1991Publication History Published online1 May 2002Published inissue 1 June 1991https://pubs.acs.org/doi/10.1021/j100166a055https://doi.org/10.1021/j100166a055research-articleACS PublicationsRequest reuse permissionsArticle Views108Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Bettering operation of Robots by learning (1984)

Abstract This article proposes a betterment process for the operation of a mechanical robot in a sense that it betters the next operation of a robot by using the previous operation's data. The process has an iterative learning structure such that the ( k + 1)th input to joint actuators consists of the k th input plus an error increment composed of the derivative difference between the k th motion trajectory and the given desired motion trajectory. The convergence of the process to the desired motion trajectory is assured under some reasonable conditions. Numerical results by computer simulation are presented to show the effectiveness of the proposed learning scheme.
A Short Synthesis of 2-Methoxy- carbonylmethyl-3-methyl-2-cyclopenten-l-one (1978)

Journal Article A Short Synthesis of 2-Methoxy- carbonylmethyl-3-methyl-2-cyclopenten-l-one Get access Mitsuru Nakayama, Mitsuru Nakayama Department of Chemistry, Faculty of Science, Hiroshima University, Higashisenda-machi, Hiroshima 730 Search for other works by this author on: Oxford Academic Google Scholar Susumu Ohira, Susumu Ohira Department of Chemistry, Faculty of Science, Hiroshima University, Higashisenda-machi, Hiroshima 730 Search for other works by this author on: Oxford Academic Google Scholar Shuzo Shinke, Shuzo Shinke Department of Chemistry, Faculty of Science, Hiroshima University, Higashisenda-machi, Hiroshima 730 Search for other works by this author on: Oxford Academic Google Scholar Shûichi Hayashi Shûichi Hayashi Department of Chemistry, Faculty of Science, Hiroshima University, Higashisenda-machi, Hiroshima 730 Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 42, Issue 12, 1 December 1978, Pages 2399–2400, https://doi.org/10.1080/00021369.1978.10863369 Published: 01 December 1978 Article history Received: 26 June 1978 Published: 01 December 1978
An adaptive resonant filter (1970)

A solid-state adaptive (analog storage) device with stable electrical characteristics is described and demonstrated. The device is a resonant bandpass electronic filter with adaptable voltage gain; that is, the voltage gain-frequency transfer characteristic can be "set" to different values of attenuation by the application of an adapt signal and will retain that "setting" after the adapt signal has been removed. Ferroelectric materials are used as the dielectric in a filter structure composed of two capacitors bonded together so that resonant mechanical vibrations established in one (the input resonator) are coupled to the other (the output resonator). Converse and direct piezoelectric effects generate the mechanical vibrations and the output voltages, respectively. Ferroelectric effects in either capacitor provide the analog storage capabilities. The acoustical coupling mechanism employed in the device design results in electrically stable device characteristics. Previous ferroelectric adaptive devices used unstable field effect coupling mechanisms which led to unacceptable device performance. Experimental adaptive resonant filters fabricated with ceramic lead zirconate-lead titanate material compositions are discussed. These filters have electronic Q values near 100 at resonant frequencies in the range 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> to 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">7</sup> Hz. The voltage gain-frequency characteristic has a maximum value at resonance of about 0 to +10 dB. Application of a voltage adapt pulse (100 to 300 volts) of low energy (mJ) to either side of the filter can adapt the entire gain characteristic by any value between 0 and about -60 dB within an arbitrary switching time (limited to a practical range of roughly 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+3</sup> to 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-4</sup> seconds) as determined by the pulse amplitude. Voltage gain settings are electrically stable and can be reproduced by the same or an equivalent sequence of adapting pulses.
TACITE: A Transient Tool for Multiphase Pipeline and Well Simulation (1994)

Abstract The paper presents the principal characteristics of the TACITE code which has been developed to simulate transient and steady state multiphase flows, for the design and control of oil and gas production networks. The hydraulic model is presented briefly and the resulting flow regime prediction is compared with experimental data. The performance of the code is illustrated with respect to steady state and transient experimental and field data.