gebhart/patrepeval huggingface dataset:
In Vitro Cytotoxicitiy of Silica Nanoparticles at High Concentrations Strongly Depends on the Metabolic Activity Type of the Cell Line (2007)

Amorphous silica is increasingly used in diagnostic and biomedical research because of its ease of production and relatively low cost. It is generally regarded as safe and has been approved for use as a food or animal feed ingredient. Recent literature reveals that amorphous silica may present toxicity concerns at high doses. In anticipation of potential human exposure to silica, it is advisable to examine its toxicity to cells of different organs. Consequently, we investigated the response of several normal fibroblast and tumor cells to varying doses of amorphous silica or composite nanoparticles of silica and chitosan. A cell proliferation assay indicates that silica nanoparticles are nontoxic at low dosages but that cell viability decreases at high dosages. A lactate dehydrogenase (LDH) assay indicates that high dosages of silica induce cell membrane damage. Both assays reveal that fibroblast cells with long doubling times are more susceptible to injury induced by silica exposure than tumor cells with short doubling times. In contrast, silica-chitosan composite nanoparticles induce less inhibition in cell proliferation and less membrane damage. This study suggests that the cytotoxicity of silica to human cells depends strongly on their metabolic activities but that it could be significantly reduced by synthesizing silica with chitosan.
Terpenoids from Endophytic Fungi (2011)

This work reviews the production of terpenoids by endophytic fungi and their biological activities, in period of 2006 to 2010. Sixty five sesquiterpenes, 45 diterpenes, five meroterpenes and 12 other terpenes, amounting to 127 terpenoids were isolated from endophytic fungi.
The Sun Health Research Institute Brain Donation Program: Description and Eexperience, 1987–2007 (2008)

The Brain Donation Program at Sun Health Research Institute has been in continual operation since 1987, with over 1000 brains banked. The population studied primarily resides in the retirement communities of northwest metropolitan Phoenix, Arizona. The Institute is affiliated with Sun Health, a nonprofit community-owned and operated health care provider. Subjects are enrolled prospectively to allow standardized clinical assessments during life. Funding comes primarily from competitive grants. The Program has made short postmortem brain retrieval a priority, with a 2.75-h median postmortem interval for the entire collection. This maximizes the utility of the resource for molecular studies; frozen tissue from approximately 82% of all cases is suitable for RNA studies. Studies performed in-house have shown that, even with very short postmortem intervals, increasing delays in brain retrieval adversely affect RNA integrity and that cerebrospinal fluid pH increases with postmortem interval but does not predict tissue viability.
Pressure Sensitive Adhesives Based on Oleic Acid (2014)

Abstract Existing pressure sensitive adhesives (PSA) are mostly based on petrochemical‐based polymers. This study reveals a new class of bio‐based polymers that can be used as PSA. The polymers are hydroxyl‐containing polyesters from the step‐growth polymerization of epoxidized oleic acid (EOA), an AB‐type monomer containing both a carboxylic acid group (A) and an epoxy group (B). The monomer is derived from epoxidation of renewable methyl oleate followed by selective hydrolysis of the ester group. The polymers (PEOA) of EOA were characterized for their chemical structure and molecular weight. The PEOA after being cured with a very small amount of a crosslinking agent could serve as a PSA with high peel strength, high tack force, superior shear resistance, excellent aging resistance, and excellent thermal stability. The PSA contains 99 wt% of green renewable materials. The PSA were also characterized for their viscoelastic properties and thermal properties.
An In Vitro Analysis of Myocardial Potential Indicates That Phenotypic Plasticity Is an Innate Property of Early Embryonic Tissue (2004)

Explants from gastrula-stage avian embryos have provided an important culture model for examining the formation of the vertebrate heart. Explants harvested from anterior regions containing the precardiac mesoderm faithfully recapitulate cardiogenesis and generate contractile tissue in culture. Posterior regions of the early embryo do not supply cellular material to the developing heart in situ, and thus have been commonly employed as negative control tissues for studying cardiogenic induction. To begin to understand the cellular mechanisms that account for the distinct cell fates of precardiac and posterior tissue within the embryo, we undertook a comprehensive investigation on the myocardial potential of presumptive noncardiac tissue. Myocardial differentiation was assayed by expression of the myocardium-associated transcription factor gene Nkx2.5 and positive immunostaining for sarcomeric myosin, muscle alpha-actinin, and smooth muscle alpha-actin. Our results demonstrate that regions of the early embryo that do not provide a cellular contribution to the myocardium in situ are capable of generating myocardial tissue when removed from their normal embryonic environment and placed in culture under nontreated conditions. Although treatment with the presumptive cardiac inducer Dickkopf-1 increased the frequency that cardiac tissue appeared within cultures of posterior tissue, no difference was observed in either the size or morphology of the myocardium-positive areas among treated and nontreated explants. These findings suggest that progenitor cells within the early embryo possess an innate phenotypic plasticity and that presumptive cardiac inducing signals do not induce cardiac differentiation but instead augment a pre-existing cardiac potential of embryonic tissue.
iRotateGrasp (2013)

Automatic screen rotation improves viewing experience and usability of mobile devices, but current gravity-based approaches do not support postures such as lying on one side, and manual rotation switches require explicit user input. iRotateGrasp automatically rotates screens of mobile devices to match users' viewing orientations based on how users are grasping the devices. Our prototype used a total of 44 capacitive sensors along the four sides and the back of an iPod Touch, and uses support vector machine (SVM) to recognize grasps at 25Hz. We collected 6-users' usage under 108 different combinations of posture, orientation, touchscreen operation, and left/right/both hands. Our offline analysis showed that our grasp-based approach is promising, with 80.9% accuracy when training and testing on different users, and up to 96.7% if users are willing to train the system.
A Pilot Study to Improve Venipuncture Compliance in Children and Adolescents with Autism Spectrum Disorders (2011)

Results indicate the efficacy of the program in a research setting and suggest a potential clinical application. The current intervention, unlike many others for the same or similar difficulties proposed in the past, was successful without requiring extensive time, training, or effort on the part of providers and parents or their children, nor did it require large-scale institutional changes.
Immunoprotection against enterotoxigenicEscherichia colidiarrhea in rabbits by peroral administration of purified colonization factor antigen I (CFA/I) (1981)

Development of reliable methods for producing artificially induced immunoprotection against the enterotoxigenic enteropathogens Vibrio cholerae and enterotoxigenic Escherichia coil (ETEC) has been the subject of much investigation [1-6]. Numerous experiments with cholera vibrios, cholera toxin, various cholera toxoids and ETEC heat-labile enterotoxin (LT) as immunogens in various animal species have been reported [25,7,8]. These studies generally lead to the conclusion that immunoprotection against V. cholerae and ETEC diarrhea is primarily dependent upon stimulation of an adequate local immune response in the intestine, the primary mechanism being secretion of specific secretory IgA antibody [2-6,9]. In approaching the problem of immunoprophylaxis against ETEC diarrhea, consideration must be given to the choice of antigens for study. Since numerous different ETEC serotypes produce antigenically identical LT [10,11] this antigen is considered to be a good candidate as an immunogen. On the other hand, since the first step in ETEC infection is intestinal colonization it follows that protection from colonization should be equivalent
Effects of Medical Marijuana on Migraine Headache Frequency in an Adult Population (2016)

The frequency of migraine headache was decreased with medical marijuana use. Prospective studies should be conducted to explore a cause-and-effect relationship and the use of different strains, formulations, and doses of marijuana to better understand the effects of medical marijuana on migraine headache treatment and prophylaxis.
Chemokine and receptor-gene expression during early and late acute rejection episodes in human cardiac allografts (2003)

IP-10, Mig, RANTES, CXCR3, and CCR5 expression levels were increased in the later versus earlier biopsies (P< or =0.01) despite no change in histologic rejection-grade status. CONCLUSION These results demonstrate significantly increased expression of T-cell chemoattractants in heart allografts during later rejection when compared with episodes occurring shortly after transplantation. The findings suggest increased intensity of inflammation in rejection occurring at later times posttransplant that are revealed by molecular analyses of the graft.
Identification of the Function of Gene lndM2 Encoding a Bifunctional Oxygenase-Reductase Involved in the Biosynthesis of the Antitumor Antibiotic Landomycin E by Streptomyces globisporus 1912 Supports the Originally Assigned Structure for Landomycinone (2004)

The angucycline antibiotic family of the landomycins displays potent antitumor activity. To elucidate early post polyketide synthase (PKS) tailoring steps of the landomycin E biosynthetic pathway in Streptomyces globisporus 1912, the mutant S. globisporus M12 was prepared through gene replacement experiment of lndM2. It encodes an enzyme with putative oxygenase and reductase domains, according to sequencing of the gene and its counterpart lanM2 from S. cyanogenus S136 landomycin A biosynthetic gene cluster. The isolation of the novel shunt products 11-hydroxytetrangomycin and 4-hydroxytetrangomycin along with the well-known angucyclines tetrangomycin and tetrangulol from the culture of S. globisporus M12 provides evidence for the involvement of lndM2 in the early biosynthetic pathway of the landomycins, in particular in the formation of the alicyclic 6-hydroxy function of the landomycin aglycon. We therefore propose LndM2 to be responsible for both hydroxylation of the 6-position and its subsequent reduction. These reactions are necessary before the glycosylation reactions can occur. The results are in agreement with the originally published structure of landomycin but do not support the recently suggested revised structure.
Accelerating K-Means clustering with parallel implementations and GPU computing (2015)

K-Means clustering is a popular unsupervised machine learning method which has been used in diverse applications including image processing, information retrieval, social sciences and weather forecasting. However, clustering is computationally expensive especially when applied to large datasets. In this paper, we explore accelerating the performance of K-means clustering using three approaches: 1) shared memory using OpenMP, 2) distributed memory with message passing (MPI), and 3) heterogeneous computing with NVIDIA Graphics Processing Units (GPUs) programmed with CUDA-C. While others have looked at accelerating K-means clustering, this is the first study that compares these different approaches. In addition, K-means performance is very sensitive to the initial means chosen. We evaluate different initializations in parallel and choose the best one to use for the entire algorithm. We evaluate results on a range of images from small (300×300 pixels) to large (1164×1200 pixel). Our results show that all three parallel programming approaches give speed-up, with the best results obtained by OpenMP for smaller images and CUDA-C for larger ones. Each of these approaches gives approximately thirty times overall speed-up compared to a sequential implementation of K-means. In addition, our parallel initialization gives an additional 1.5 to 2.5 times speed-up over the accelerated parallel versions.
Polyvinyl alcohol particle size and suspension characteristics. (1995)

The three dry PVA preparations seem to be similar. All increase significantly in size when suspended in nonionic contrast or contrast-alcohol solutions. The saline-packaged PVA particles were different from the dry variety and did not enlarge in contrast or contrast-alcohol solutions. Alcohol did not change the size or suspension characteristics of PVA particles. Particles less than 50 microns in size were rarely identified.
The occurrence and metabolism of alkyl and alk-1-enyl ethers of glycerol in transplantable rat and mouse tumors. (1968)

SUMMARY Thirteen transplantable animal tumors have been quantita tively analyzed for their glyceryl alkyl and alk-1-enyl ethers in the neutral lipid and phospholipid fractions. The data ob tained demonstrate that the quantity of glyceryl ethers in the neutral lipid fraction of tumors is significantly higher than that found in normal tissues. The amount of ether-linked phospholipids is also higher in tumors than in most normal tissues. Radioactivity from intraperitoneal injections of palmitic acid-l-14C was incorporated into both the ether-linked aliphatic moiety (13-25% of the total glyceryl ether diester activity) and the esterified fatty acids of glyceryl ether diesters isolated from Ehrlich ascites cells. Labeling of the glyceryl ether diesters also occurred when fatty alcohols-14C and triglycerides-14C were administered to mice bearing the ascites cells; radio activity from acetate-2-14C, glucose-14C, and 3H20 was not incorporated into the glyceryl ether diesters. The specific ac tivity of the triglycA©ride fraction was always higher than that of the glyceryl ether diesters found in Ehrlich ascites cells after injections of palmitic acid-l-14C. In similar experiments, the specific activities of the alk-1-enyl ethers were always higher than the specific activities of the alkyl ethers in the neutral lipid fraction, but these specific activities were essentially the same in the phospholipid fraction.
Practical Machine Learning: A New Look at Anomaly Detection (2014)

Anomaly detection is the detective work of machine learning: finding the unusual, catching the fraud, discovering strange activity in large and complex datasets. But, unlike Sherlock Holmes, you may not know what the puzzle is, much less what suspects you're looking for. This O'Reilly report uses practical examples to explain how the underlying concepts of anomaly detection work. From banking security to natural sciences, medicine, and marketing, anomaly detection has many useful applications in this age of big data. And the search for anomalies will intensify once the Internet of Things spawns even more new types of data. The concepts described in this report will help you tackle anomaly detection in your own project. Use probabilistic models to predict what's normal and contrast that to what you observe Set an adaptive threshold to determine which data falls outside of the normal range, using the t-digest algorithm Establish normal fluctuations in complex systems and signals (such as an EKG) with a more adaptive probablistic model Use historical data to discover anomalies in sporadic event streams, such as web traffic Learn how to use deviations in expected behavior to trigger fraud alerts
Sequence‐Dependent Assembly to Control Molecular Interface Properties (2012)

Variation's what you need: variation of the assembly sequence in which layers of two isostructural metal complexes are built up leads to molecular materials with electrochemical properties that depend on the assembly sequence. These properties vary from reversible electron transfer to unidirectional current flows and even charge trapping. The sequence-dependent assembly strategy has implications for various disciplines that involve self-assembly.
Bcl-2 Controls Dendritic Cell Longevity In Vivo (2002)

Dendritic cells (DC) were found to down-regulate Bcl-2 protein upon maturation in vivo. Because Bcl-2 has been shown to exert anti-apoptotic functions, down-regulation of Bcl-2 could be a mechanism by which DC longevity is controlled. To dysregulate this potential control system and to study the role of Bcl-2 in DC, we expressed human Bcl-2 under control of the murine CD11c-promoter as a transgene specifically in DC and show that DC frequencies and numbers increase in transgenic mice. In vivo bromodeoxyuridin, as well as adoptive, DC transfer studies show that the relative turnover/survival of mature Bcl-2 transgenic DC is increased. This had a direct impact on CD4+ T cell, as well as humoral immune, responses, which were elevated in transgenic animals. When Bcl-2 transgenic DC were used as DC vaccines, they induced 2- to 3-fold greater expansion of Ag-specific CTL, and stronger in vivo cytotoxicity. Overall, these data indicate that down-regulation of Bcl-2 controls DC longevity, which in turn directly regulates immune responses and the efficacy of DC when used as vaccines.
Design, fabrication andin vitroevaluation of a novel polymer-hydrogel hybrid scaffold for bone tissue engineering (2012)

The development of a bone mechanically-compatible and osteoinductive scaffold is important for bone tissue engineering applications, particularly for the repair and regeneration of large area critically-sized bone defects. Although previous studies with weight-bearing scaffolds have shown promising results, there is a clear need to develop better osteoinductive strategies for effective scaffold-based bone regeneration. In this study, we designed and fabricated a novel polymer-hydrogel hybrid scaffold system in which a load-bearing polymer matrix and a peptide hydrogel allowed for the synergistic combination of mechanical strength and great potential for osteoinductivity in a single scaffold. The hybrid scaffold system promoted increased pre-osteoblastic cell proliferation. Further, we biotinylated human recombinant bone morphogenetic protein 2 (rhBMP2), and characterized the biotin addition and its effect on rhBMP2 biological activity. The biotinylated rhBMP2 was tethered to the hybrid scaffold using biotin-streptavidin complexation. Controlled release studies demonstrated increased rhBMP2 retention with the tethered rhBMP2 hybrid scaffold group. In vitro evaluation of the hybrid scaffold was performed with rat bone marrow stromal cells and mouse pre-osteoblast cell line MC3T3-E1 cells. Gene expression of alkaline phosphatase (ALP), collagen I (Col I), osteopontin (OPN), bone sialoprotein (BSP), Runx-2 and osteocalcin (OC) increased in MC3T3-E1 cells seeded on the rhBMP2 tethered hybrid scaffolds over the untethered counterparts, demonstrating osteoinductive potential of the hybrid graft. These findings suggest the possibility of developing a novel polymer-hydrogel hybrid system that is weight bearing and osteoinductive for effective bone tissue engineering.
Characteristics of ZnMgO Thin Films Prepared by the Sol-Gel Method (2009)

Mg-doped (≤15at%) ZnO thin films were prepared on quartz glass substrates by sol-gel method using a spinning-techniques. The films were charactered by X-ray diffraction , UV Vis-NIR double beam spectrophotometer and a SHIMADZU fluorespectrometer. It was found that optimally crystallized when the Mg concentration is 9at% were obtained. A photoluminescence study at room temperature revealed a blue shift in the near band edge emission peak with increasing of Mg content , which is agreement with the observation from the optical transmittance measurement. The photoconductive UV detectors based on these films were investigated, and the result showed that highest response was obtained from the photodecters fabricated by films with 15at% Mg concentration.
NURB curves and surfaces: from projective geometry to practical use (1995)

NURBS (Non-uniform rational B-splines) have become a de facto standard for geometric definition in CAD/CAM and computer graphics. This book covers NURBS from their geometric beginnings to their industrial applications. The text begins with an introduction to projective geometry for which only an elementary background in linear algebra is necessary. Conics are then treated in terms of projective geometry as well as rational quadratic NURBS. A similar treatment is given to the general case of NURBS curves and surfaces. Each chapter concludes with a set of problems.
Genome organization of the killer plasmid pGK12 from Kluyveromyces lactis (1988)

We have determined the entire sequence of the plasmid K2 from Kluyveromyces lactis which is involved in the maintenance of both killer plasmids in the cell. K2 shares many of the characteristics of the smaller killer plasmid K1: high A+T content (74.7%) and very compact genomic organization. K2 contains ten open reading frames. Some of them overlap on different strands and some on the same strand. Northern blotting of K2 transcripts shows that at least eight ORFs are transcribed. Analysis of the predicted aminoacid sequence of ORF2 from K2 reveals homology with the aminoacid sequence of ORF 1 from K1 and with several viral DNA polymerases. The sequence of K2 from Saccaromyces cerevisiae F102-2 was also determined. Only one nucleotide difference was found between the K2 sequence from the two yeasts. This mutation does not change the genome organization of the plasmid and has only minimal effect on the structure of the encoded proteins.
Analysis of Peptidoglycan Structure from Vegetative Cells of Bacillus subtilis 168 and Role of PBP 5 in Peptidoglycan Maturation (1999)

The composition and fine structure of the vegetative cell wall peptidoglycan from Bacillus subtilis were determined by analysis of its constituent muropeptides. The structures of 39 muropeptides, representing 97% of the total peptidoglycan, were elucidated. About 99% analyzed muropeptides in B. subtilis vegetative cell peptidoglycan have the free carboxylic group of diaminopimelic acid amidated. Anhydromuropeptides and products missing a glucosamine at the nonreducing terminus account for 0.4 and 1.5%, respectively, of the total muropeptides. These two types of muropeptides are suggested to end glycan strands. An unexpected feature of B. subtilis muropeptides was the occurrence of a glycine residue in position 5 of the peptide side chain on monomers or oligomers, which account for 2.7% of the total muropeptides. This amount is, however, dependent on the composition of the growth media. Potential attachment sites for anionic polymers to peptidoglycan occur on dominant muropeptides and account for 2.1% of the total. B. subtilis peptidoglycan is incompletely digested by lysozyme due to de-N-acetylation of glucosamine, which occurs on 17.3% of muropeptides. The cross-linking index of the polymer changes with the growth phase. It is highest in late stationary phase, with a value of 33.2 or 44% per muramic acid residue, as determined by reverse-phase high-pressure liquid chromatography or gel filtration, respectively. Analysis of the muropeptide composition of a dacA (PBP 5) mutant shows a dramatic decrease of muropeptides with tripeptide side chains and an increase or appearance of muropeptides with pentapeptide side chains in monomers or oligomers. The total muropeptides with pentapeptide side chains accounts for almost 82% in the dacA mutant. This major low-molecular-weight PBP (DD-carboxypeptidase) is suggested to play a role in peptidoglycan maturation.