gebhart/patrepeval huggingface dataset:
Pseudorabies virus glycoprotein gIII is a major target antigen for murine and swine virus-specific cytotoxic T lymphocytes (1990)

Pseudorabies virus (PrV) is the etiological agent of Aujeszky's disease, a disease that causes heavy economic losses in the swine industry. A rational approach to the generation of an effective vaccine against this virus requires an understanding of the immune response induced by it and of the role of the various viral antigens in inducing such a response. We have constructed mutants of PrV [strain PrV (Ka)] that differ from each other only in expression of the viral nonessential glycoproteins gI, gp63, gX, and gIII (i.e., are otherwise isogenic). These mutants were used to ascertain the importance of each of the nonessential glycoproteins in eliciting a PrV-specific cytotoxic T-lymphocyte (CTL) response in mice and pigs. Immunization of DBA/2 mice and pigs with a thymidine kinase-deficient (TK-) mutant of PrV elicits the formation of cytotoxic cells that specifically lyse syngeneic infected target cells. These PrV-specific cytolytic cells have the phenotype of major histocompatibility complex class I antigen-restricted CTLs. The relative number of CTLs specific for glycoproteins gI, gp63, gX, and gIII induced in mice vaccinated with a TK- mutant of PrV was ascertained by comparing their levels of cytotoxicity against syngeneic cells infected with either wild-type virus or gI-/gp63-, gX-, or gIII- virus deletion mutants. The PrV-specific CLTs were significantly less effective in lysing gIII(-)-infected targets than in lysing gI-/gp63-, gX-, or wild-type-infected targets. The in vitro secondary CTL response of lymphocytes obtained from either mice or pigs 6 or more weeks after immunization with a TK- mutant of PrV was also tested. Lymphocytes obtained from these animals were cultured with different glycoprotein-deficient mutants of PrV, and their cytolytic activities against wild-type-infected targets were ascertained. The importance of each of the nonessential viral glycoproteins in eliciting CTLs was assessed from the effectiveness of each of the virus mutants to stimulate the secondary anti-PrV CTL response. Cultures of both murine or swine lymphocytes that had been stimulated with gIII- virus contained only approximately half as many lytic units as did those stimulated with either wild-type virus, a gX- virus mutant, or a gI-/gp63- virus mutant. Thus, a large proportion of the PrV-specific CTLs that are induced by immunization with PrV of both mice and pigs are directed against gIII. Furthermore, glycoproteins gI, gp63, and gX play at most a minor role in the CTL response of these animals to PrV.
Human memory T lymphocytes express increased levels of three cell adhesion molecules (LFA-3, CD2, and LFA-1) and three other molecules (UCHL1, CDw29, and Pgp-1) and have enhanced IFN- gamma production. (1988)

Studies of cell-surface molecules involved in human T cell interaction reveal that differential expression of each of three adhesion molecules (LFA-3, CD2, and LFA-1) subdivides human peripheral blood T cells into major subpopulations. Systematic analysis of the relationship between expression of these and other markers of T cell subsets demonstrates a single major subset of human peripheral blood T lymphocytes distinguished by enhanced expression of LFA-3, CD2, LFA-1, and three other markers (CDw29 [4B4], UCHL1, and Pgp-1). Large differences in relative expression are observed for UCHL1 (29-fold) and LFA-3 (greater than 8-fold), and smaller differences (2- to 4-fold) are seen for CDw29, CD2, LFA-1, and Pgp-1. Bimodal distribution of LFA-3 is found on both CD4+ cells and on CD8+ cells as well as on B lymphocytes (CD19+). Neonatal T cells (CD3+) are comprised almost exclusively of the subset expressing low LFA-3, CD2, LFA-1, CDw29, and UCHL1. Activation of cord peripheral blood mononuclear leukocytes with PHA leads to uniform enhanced expression of each of these molecules on CD3+ cells. Functional analyses of these T cell subsets were performed after sorting of adult T cells based on differential LFA-3 expression. Only the LFA-3+ subset proliferated in response to the Ag tetanus toxoid, even though the LFA-3- subset proliferated more strongly to PHA. Furthermore, the LFA-3+ subset made greater than fivefold more IFN-gamma than the LFA-3- subset in response to PHA, despite the fact that both subsets made equivalent amounts of IL-2. This phenotypic and functional analysis of resting and activated newborn and adult T cells indicates that human memory T cells express enhanced levels of LFA-3, CD2, LFA-1, UCHL1, CDw29, and Pgp-1; we speculate that the increase in expression of T cell adhesion molecules LFA-3, CD2, and LFA-1 on memory cells is functionally important in their enhanced responsiveness.
Fast Rhythmic Bursting Can Be Induced in Layer 2/3 Cortical Neurons by Enhancing Persistent Na+Conductance or by Blocking BK Channels (2003)

Fast rhythmic bursting (or "chattering") is a firing pattern exhibited by selected neocortical neurons in cats in vivo and in slices of adult ferret and cat brain. Fast rhythmic bursting (FRB) has been recorded in certain superficial and deep principal neurons and in aspiny presumed local circuit neurons; it can be evoked by depolarizing currents or by sensory stimulation and has been proposed to depend on a persistent g(Na) that causes spike depolarizing afterpotentials. We constructed a multicompartment 11-conductance model of a layer 2/3 pyramidal neuron, containing apical dendritic calcium-mediated electrogenesis; the model can switch between rhythmic spiking (RS) and FRB modes of firing, with various parameter changes. FRB in this model is favored by enhancing persistent g(Na) and also by measures that reduce [Ca(2+)](i) or that reduce the conductance of g(K(C)) (a fast voltage- and Ca(2+)-dependent conductance). Axonal excitability plays a critical role in generating fast bursts in the model. In vitro experiments in rat layer 2/3 neurons confirmed (as shown previously by others) that RS firing could be switched to fast rhythmic bursting, either by buffering [Ca(2+)](i) or by enhancing persistent g(Na). In addition, our experiments confirmed the model prediction that reducing g(KC) (with iberiotoxin) would favor FRB. During the bursts, fast prepotentials (spikelets) could occur that did not originate in apical dendrites and that appear to derive from the axon. We suggest that modulator-induced regulation of [Ca(2+)] dynamics or of BK channel conductance, for example via protein kinase A, could play a role in determining the firing pattern of neocortical neurons; specifically, such modulation could play a role in regulating whether neurons respond to strong stimulation with fast rhythmic bursts.
Aging-induced wavelength shifts in 1.5-µm DFB lasers (1994)

Recent achievements in optical amplifiers revitalize the practicality of wavelength-division-multiplexing (WDM) systems. However, WDM systems require long-term wavelength stability of transmitter laser to avoid cross talk. Various frequency-stabilization schemes have been proposed.1,2 However, frequency stabilization increases cost and complexity. Recently, it has been reported that the aging-induced wavelength shifts of six commercial 1.5-μm distributed-feedback (DFB) transmitter lasers over a period of 16 months were measured to be negligible (&lt;0.03 Å).3 In this paper we report our extensive investigation on the aging-induced wavelength shifts in 1.5-μm DFB lasers.
Identification of an exon 3 deletion splice variant androgen receptor mRNA in human breast cancer (1997)

Androgens and androgen receptor (AR) are involved in many regulatory processes in the growth of female breast cells. Mutations in the AR gene and/or alterations of the AR protein sequence may be related to the development and progression of breast cancer. Using reverse transcription-polymerase chain reaction we have examined 31 female breast-cancer samples, 5 normal female breast tissues and 6 breast-cancer cell lines for the presence of splice variants of AR mRNA and have identified an exon 3 deletion splice variant (delta3AR). The higher expression of the variant relative to the wild-type AR (WT AR) was found in 7 breast-cancer samples (delta3/WT > 15%) and relatively lower levels of the variant were observed in 3 breast-cancer cell lines (delta3/WT < 5%). However, in normal breast tissues, expression of the variant was undetectable by Southern blot analysis. In vitro translation of the delta3AR mRNA resulted in a variant AR protein of about 105 kDa, smaller than the WT AR by about 5 kDa. We thus report an exon deletion splice variant of AR mRNA in breast cancer. The variant protein is predicted to lack the second zinc finger within the DNA-binding domain and is expected to be unable or to have reduced ability to bind to androgen-response elements and to activate transcription. The relatively high expression of this AR variant in some breast-cancer tissues may indicate its role in regulating the growth of these cancers.
Exhaustive Matching of the Entire Protein Sequence Database (1992)

The entire protein sequence database has been exhaustively matched. Definitive mutation matrices and models for scoring gaps were obtained from the matching and used to organize the sequence database as sets of evolutionarily connected components. The methods developed are general and can be used to manage sequence data generated by major genome sequencing projects. The alignments made possible by the exhaustive matching are the starting point for successful de novo prediction of the folded structures of proteins, for reconstructing sequences of ancient proteins and metabolisms in ancient organisms, and for obtaining new perspectives in structural biochemistry.
Holographic Information Retrieval System (1974)

We describe in this paper the organization and operation of an exploratory holographic library information processing system successfully demonstrated in our laboratory. This system consists of a recording stage and an information-retrieval and readout stage, all of which are controlled by a minicomputer. Abstracts of literature are stored in hologram arrays on 35-mm roll film. A new technique of holographic correlation detection that is appropriate for parallel processing is used for interrogations with arbitrary words, and it results in the remarkable reduction in the loads of the minicomputer and its interface to the optical system.
Disease expression in X-linked retinitis pigmentosa caused by a putative null mutation in the RPGR gene. (1997)

This RPGR gene mutation, in its mildest expression in heterozygotes, causes a relatively equal disturbance of rod and cone photoreceptor function. Detectable structural change by OCT at the level of the RPE-CC can be present in patches of retina with minimal functional disturbance. More advanced disease stages in heterozygotes show greater rod than cone dysfunction, and the end stage in hemizygotes and heterozygotes is that of typical RP, with only barely detectable cone function from residual cones in a thinned retina with abnormal RPE and choriocapillaris.
Bis(heteroaryl)piperazine (BHAP) reverse transcriptase inhibitors: structure-activity relationships of novel substituted indole analogs and the identification of 1-[(5-methanesulfonamido-1H-indol-2-yl)carbonyl]-4-[3-[(1-methylethyl)amino]pyridinyl]piperazinemonomethanesulfonate (U-90152S), a second-generation clinical candidate (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBis(heteroaryl)piperazine (BHAP) reverse transcriptase inhibitors: structure-activity relationships of novel substituted indole analogs and the identification of 1-[(5-methanesulfonamido-1H-indol-2-yl)carbonyl]-4-[3-[(1-methylethyl)amino]pyridinyl]piperazinemonomethanesulfonate (U-90152S), a second-generation clinical candidateDonna L. Romero, Raymond A. Morge, Michael J. Genin, Carolyn Biles, Mariano Busso, Lionel Resnick, Irene W. Althaus, Fritz Reusser, Richard C. Thomas, and William G. TarpleyCite this: J. Med. Chem. 1993, 36, 10, 1505–1508Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/jm00062a027https://doi.org/10.1021/jm00062a027research-articleACS PublicationsRequest reuse permissionsArticle Views554Altmetric-Citations111LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A New Trend (2014)

China’s economic growth is stabilizing in the 7-8 percent range China’s gross domestic product(GDP)grew 7.8 percent in the third quarter,0.3percentage points higher than in the second quarter.Economic growth rate has been ranging from 7 to 8 percent since the second quarter of 2012.The fourth quarter of 2013 will be no exception.Therefore,the growth rate floating between 7 to 8 percent is not an accidental phenomenon but a periodical trend for the country.
Electrical Transport in Schottky Barrier MOSFETs (2001)

Electrical Transport in Schottky Barrier MOSFETs Laurie Ellen Calvet Yale University 2001 The motivation for this thesis originates in the semiconductor industry whose rapid economic growth in the past thirty years has been stimulated by scaling transistors to smaller dimensions. The current drive to design transistors beyond fundamental limitations is causing the industry to consider alternative structures. The Schottky Barrier (SB) MOSFET is one such device. It consists of metallic silicide source and drain contacts and a standard MOS gate. Previously, simulations have indicated that these transistors will have superior scaling properties and be more cost effective to fabricate than conventional transistors when channel lengths are scaled below 70 nm. This research experimentally investigates the current transport and scaling behavior of pSBMOSFETs fabricated on bulk silicon substrates. We also explore the unique low temperature properties of Schottky Barrier (SB) MOSFETs. We find evidence that single charged impurities cause ‘hot spots’ that result in a reduced local potential at the abrupt metal/semiconductor interface. At relatively high temperatures (20-100 K) the impurities give rise to a non-uniform Schottky Barrier height. At lower temperatures electrons crossing the ~ 8 nm barrier width are coherent, and a single impurity causes both enhanced direct tunneling and a resonant state due to the confined potential. As a result, we observe quantum interference between these two distinct paths of a single electron, known as a Fano resonance. Tuning of the interference is explored by varying temperature, bias direction and bias magnitude.
Membrane and Morphological Changes in Apoptotic Cells Regulated by Caspase-Mediated Activation of PAK2 (1997)

Apoptosis of Jurkat T cells induced the caspase-mediated proteolytic cleavage of p21-activated kinase 2 (PAK2). Cleavage occurred between the amino-terminal regulatory domain and the carboxyl-terminal catalytic domain, which generated a constitutively active PAK2 fragment. Stable Jurkat cell lines that expressed a dominant-negative PAK mutant were resistant to the Fas-induced formation of apoptotic bodies, but had an enhanced externalization of phosphatidylserine at the cell surface. Thus, proteolytic activation of PAK2 represents a guanosine triphosphatase-independent mechanism of PAK regulation that allows PAK2 to regulate morphological changes that are seen in apoptotic cells.
Electrooptic light modulators (1966)

The field of electrooptic light modulation by means of the Pockels and Kerr effects in crystals is summarized with particular attention to communications applications using the optical maser. AH available data on electrooptic materials are tabulated, and design considerations and operating principles for various modulator configurations are outlined.
Structure and Transcriptional Regulation of Human α‐Mannosidase IIx (α‐Mannosidase II Isotype) Gene (1996)

Golgi alpha-mannosidase II is a key enzyme of N-glycan processing. Its genetic defect is associated with HEMPAS (hereditary erythroblastic multinuclearity with positive acidified serum lysis test). We previously cloned cDNAs of human alpha-mannosidase II (alpha-MII) and its isotype, alpha-mannosidase IIX [alpha-MIIX, Misago, M., Liao, Y. F., Eto, S., Mattei. M. G., Moremen. K. W. & Fukuda, M. N. (1995) Proc. Natl Acad. Sci. USA 92, 11766-11770]. Constitutive expressions of alpha-MII and alpha-MIIX mRNA were shown in various human tissues. To investigate the transcriptional regulation of alpha-MIIX gene, we characterized the cosmid clone of 40-kb that includes the 5'-flanking sequence. This clone contains at least eight exons which encode 396 amino acid residues of a total of 1139 amino acid residues of alpha-MIIX. Primer-extension analysis revealed multiple transcription-initiation sites in the range from -70 to -58 relative to the translation-initiation site. No canonical TATA or CAAT boxes were observed, but a (G + C)-rich region was found in close proximity to the transcription-initiation site. To localize the transcriptional regulatory region of this gene, various regions of the 5' sequences were fused to the luciferase gene, and transient-expression assays were conducted in human melanoma G-361 cells. These studies indicated that sequence from -12 to + 11 relative to the most distal 5'-transcription-initiation site was involved in the promoter function. Within this region, the sequence GGGCGT similar to the consensus sequence of the Sp1 binding site, is present at positions -12 to -7. Enhancer activities were found in the region upstream of this site, notably from -4300 to -252. Thus, the alpha-MIIX promoter located in a CpG island is also regulated by upstream elements, indicating the complexity of alpha-MIIX gene expression.
Advanced join strategies for large-scale distributed computation (2014)

Companies providing cloud-scale data services have increasing needs to store and analyze massive data sets (e.g., search logs, click streams, and web graph data). For cost and performance reasons, processing is typically done on large clusters of thousands of commodity machines by using high level scripting languages. In the recent past, there has been significant progress in adapting well-known techniques from traditional relational DBMSs to this new scenario. However, important challenges remain open. In this paper we study the very common join operation, discuss some unique challenges in the large-scale distributed scenario, and explain how to efficiently and robustly process joins in a distributed way. Specifically, we introduce novel execution strategies that leverage opportunities not available in centralized scenarios, and others that robustly handle data skew. We report experimental validations of our approaches on Scope production clusters, which power the Applications and Services Group at Microsoft.
Regioselective Bromination of Fused Heterocyclic N-Oxides (2013)

A mild method for the regioselective C2-bromination of fused azine N-oxides is presented, employing tosic anhydride as the activator and tetra-n-butylammonium bromide as the nucleophilic bromide source. The C2-brominated compounds are produced in moderate to excellent yields and with excellent regioselectivity in most cases. The potential extension of this method to other halogens, effecting C2-chlorination with Ts(2)O/TBACl is also presented. Finally, this method could be incorporated into a viable one-pot oxidation/bromination process, using methyltrioxorhenium/urea hydropgen peroxide as the oxidant.
Ultra‐fast imaging using low flip angles and fids (1995)

A new ultra-fast imaging technique that does not place extreme demands on the speed of the gradient system is described. When used with comparable MRI systems, the rotating ultra-fast imaging sequence (RUFIS) can acquire images 4 to 5 times faster than gradient-moment nulled EPI and more than twice as fast as DUFIS, OUFIS, or BURST techniques. Because the technique uses free induction decays instead of echoes, it can be made particularly insensitive to effects of motion, flow, and diffusion. Preliminary images of turbulent flow are presented to demonstrate this insensitivity. However, with appropriate encoding, flow effects may be imaged.
Rate adaptive space-time modulation techniques for combating cochannel interference (2002)

Space-time coding is a highly effective approach for combating fading in wireless communications and improving the capacity of wireless networks. However, Catreux et al. (2000) showed that cochannel interference can degrade performance significantly in cellular systems. Here we design and demonstrate the interference suppression capability of low rate space-time codes. Optimal 1 b/s/Hz 2-space-time trellis codes with various numbers of states over Z/sub 4/ and GF(4) are obtained through search. We also propose rate adaptive space-time modulation which can increase the overall throughput. The effectiveness of this proposed method is demonstrated by using 2-space-time codes in an orthogonal frequency division multiplexing (OFDM) system with 2 transmit and 2 receive antennas.
Nanostructure Templating in Inorganic Solids with Organic Lyotropic Liquid Crystals (1999)

Various nanoscale semiconducting superlattices have been generated by direct templating in a lyotropic organic liquid crystal. These include superlattices of CdS, CdSe, and ZnS, templated in a liquid crystal formed by oligoethylene oxide oleyl ether amphiphiles and water. The semiconductor growth process copied the symmetry and characteristic dimensions of the original mesophase by avoiding growth of mineral within regularly spaced hydrophobic regions. The final product was a superlattice structure in which a mineral continuum was featured with hexagonally arranged cylindrical pores 2−3 nm in diameter and 5 nm apart. Most importantly, the superlattice morphology of the nanostructured systems in contact with the mesophase was found to be thermodynamically stable with respect to the solid lacking nanoscale features. We also found that both the morphology of features in the nanostructured solids and their dimension can be controlled through the amphiphile's molecular structure and water content in the liquid crystal. The semiconducting solids CdS, CdSe, and ZnS were all directly templated, while Ag2S, CuS, HgS, and PbS were produced only as nonfeatured solids using identical synthetic methodologies. We propose that interactions of polar segments in template molecules with the precipitated mineral and with its precursor ions are necessary conditions for direct templating. This is based on the absence of templating in the more covalent minerals and also in the presence of salts known to bind precursor ions.