gebhart/patrepeval huggingface dataset:
Human CD28−CD8+ T Cells Contain Greatly Expanded Functional Virus-Specific Memory CTL Clones (1999)

At birth, almost all human peripheral blood CD8+ T cells express the costimulatory molecule CD28. With increasing age, the proportion of CD8+ T cells that lack CD28 increases. Because the Ag specificity of CD28-CD8+ T cells has not previously been defined, we studied the contribution of CD28-CD8+ T cells to the memory CD8+ CTL response against two human persistent viruses, human CMV (HCMV) and HIV. From PBMC of healthy virus carriers we generated multiple independent CTL clones specific for defined viral peptides and sequenced their TCR beta-chains. We designed clonotypic oligonucleotides complementary to each beta-chain hypervariable sequence and quantified the size of individual immunodominant CTL clones in PBMC. Some individual CTL clones were very large, comprising up to 3.1% of all CD8+ T cells in PBMC, and were generally maintained at a stable level for months. Individual virus-specific CTL clones were consistently more abundant in purified CD28- cells than in the CD8+ population as a whole. Because CD28-CD8+ cells as a population have been reported to proliferate poorly in response to mitogen, we studied the function of these virus-specific CD28- CTL clones by quantifying the frequency of peptide-specific CTL precursors using limiting dilution analysis. CD28-CD8+ T cells contained high frequencies of functional memory CTL precursors specific for peptides of HCMV or HIV, generally higher than in the CD8+ T cell population as a whole. We conclude that in asymptomatic HCMV and HIV infection, human CD28-CD8+ T cells contain high frequencies of functional virus-specific memory CTL clones.
Restless Legs Syndrome and Periodic Limb Movements During Sleep: Diagnosis and Treatment (2007)

RLS and PLMS are common neurologic disorders and increase in prevalence with aging. These disorders can be disabling conditions, causing sleep disturbance at night and excessive sleepiness during the day. Polysomnography and the suggested immobilization test are used to support the clinical diagnosis of RLS and PLMS. Although levodopa alleviates symptoms, rebound and augmentation occur frequently, limiting the long-term usefulness of this agent. The direct dopamine receptor agonists such as pergolide, pramipexole, ropinirole, and cabergoline have largely replaced levodopa as the most effective treatment for RLS and PLMS.
spa Typing Method for Discriminating among Staphylococcus aureus Isolates: Implications for Use of a Single Marker To Detect Genetic Micro- and Macrovariation (2004)

Strain typing of microbial pathogens has two major aims: (i). to index genetic microvariation for use in outbreak investigations and (ii). to index genetic macrovariation for use in phylogenetic and population-based analyses. Until now, there has been no clear indication that one genetic marker can efficiently be used for both purposes. Previously, we had shown that DNA sequence analysis of the protein A gene variable repeat region (spa typing) provides a rapid and accurate method to discriminate Staphylococcus aureus outbreak isolates from those deemed epidemiologically unrelated. Here, using the hypothesis that the genetic macrovariation within a low-level recombinogenic species would accurately be characterized by a single-locus marker, we tested whether spa typing could congruently index the extensive genetic variation detected by a whole-genome DNA microarray in a collection of 36 isolates, which was recovered from 10 countries on four continents over a period of four decades, that is representative of the breadth of diversity within S. aureus. Using spa and coa typing, pulsed-field gel electrophoresis (PFGE), and microarray and multilocus enzyme electrophoresis (MLEE) data in molecular epidemiologic and evolutionary analyses, we determined that S. aureus likely has a primarily clonal population structure and that spa typing can singly index genetic variation with 88% direct concordance with the microarray and can correctly assign isolates to phylogenetic lineages. spa typing performed better than MLEE, PFGE, and coa typing in discriminatory power and in the degree of agreement with the microarray at various phylogenetic depths. This study showed that genetic analysis of the repeat region of protein A comprehensively characterizes both micro- and macrovariation in the primarily clonal population structure of S. aureus.
The white gene as a marker in a new P-element vector for gene transfer inDrosophila (1987)

We describe new vectors suitable for P-element mediated germ line transformation of Drosophila melanogaster using passenger genes whose expression does not result in a readily detectable phenotypic change of the transformed flies. The P-element vectors contain the white gene fused to the heat shock protein 70 (hsp70) gene promoter. Expression of the white gene rescues the white phenotype of recipient flies partly or completely even without heat treatment. Transformed descendents of most founder animals (GO) fall into two classes which are distinguishable by their orange and red eye colours. The different levels of white expression are presumably due to position effects associated with different chromosomal sites of insertion. Doubling of the gene dose in orange eyed fly stocks results in an easily visible darkening of the eye colour. Consequently, the generation of homozygous transformants is easily possible by simple inbreeding due to the phenotypic distinction of homo- and heterozygous transformants. Cloning into these P-element vectors is facilitated by the presence of polylinkers with 8 and 12 unique restriction sites.
Diamond Films Synthesized by Pulsed-Laser Assisted Hot-Filament CVD at 0.2 mbar Pressure (2001)

No abstracts
Overexpression of Apolipoprotein A-I Promotes Reverse Transport of Cholesterol From Macrophages to Feces In Vivo (2003)

Injection of 3H-cholesterol-labeled macrophage foam cells is a method of measuring reverse cholesterol transport specifically from macrophages to feces in vivo, and apoA-I overexpression promotes macrophage-specific reverse cholesterol transport.
The MRC Psycholinguistic Database (1981)

This paper describes a computerised database of psycholinguistic information. Semantic, syntactic, phonological and orthographic information about some or all of the 98,538 words in the database is accessible, by using a specially-written and very simple programming language. Word-association data are also included in the database. Some examples are given of the use of the database for selection of stimuli to be used in psycholinguistic experimentation or linguistic research.
Development of Infectious Clones for Virulent and Avirulent Pichinde Viruses: a Model Virus To Study Arenavirus-Induced Hemorrhagic Fevers (2009)

Several arenaviruses can cause hemorrhagic fever diseases (VHFs) in humans, the pathogenic mechanism of which is poorly understood due to their virulent nature and the lack of molecular clones. A safe, convenient, and economical small animal model of arenavirus hemorrhagic fever is based on guinea pigs infected by the arenavirus Pichinde (PICV). PICV does not cause disease in humans, but an adapted strain of PICV (P18) causes a disease in guinea pigs that mimics arenavirus hemorrhagic fever in humans in many aspects, while a low-passaged strain (P2) remains avirulent in infected animals. In order to identify the virulence determinants within the PICV genome, we developed the molecular clones for both the avirulent P2 and virulent P18 viruses. Recombinant viruses were generated by transfecting plasmids that contain the antigenomic L and S RNA segments of PICV under the control of the T7 promoter into BSRT7-5 cells, which constitutively express T7 RNA polymerase. By analyzing viral growth kinetics in vitro and virulence in vivo, we show that the recombinant viruses accurately recapitulate the replication and virulence natures of their respective parental viruses. Both parental and recombinant virulent viruses led to high levels of viremia and titers in different organs of the infected animals, whereas the avirulent viruses were effectively controlled and cleared by the hosts. These novel infectious clones for the PICV provide essential tools to identify the virulence factors that are responsible for the severe VHF-like disease in infected animals.
Biosynthesis of Penicillins. V.1 Substituted Phenylacetic Acid Derivatives as Penicillin Precursors (1948)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiosynthesis of Penicillins. V.1 Substituted Phenylacetic Acid Derivatives as Penicillin PrecursorsJoseph W. Corse, Reuben G. Jones, Quentin F. Soper, Calvert W. Whitehead, and Otto K. BehrensCite this: J. Am. Chem. Soc. 1948, 70, 9, 2837–2843Publication Date (Print):September 1, 1948Publication History Published online1 May 2002Published inissue 1 September 1948https://doi.org/10.1021/ja01189a001RIGHTS & PERMISSIONSArticle Views539Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (831 KB) Get e-Alerts Get e-Alerts
Population Growth Dynamics of Carbon Nanotubes (2011)

Understanding the population growth behavior of filamentary nanostructures, such as carbon nanotubes (CNTs), is hampered by the lack of characterization techniques capable of probing statistical variations with high spatial resolution. We present a comprehensive methodology for studying the population growth dynamics of vertically aligned CNT forests, utilizing high-resolution spatial mapping of synchrotron X-ray scattering and attenuation, along with real-time height kinetics. We map the CNT alignment and dimensions within CNT forests, revealing broadening and focusing of size distributions during different stages of the process. Then, we calculate the number density and mass density of the CNT population versus time, which are true measures of the reaction kinetics. We find that the mass-based kinetics of a CNT population is accurately represented by the S-shaped Gompertz model of population growth, although the forest height and CNT length kinetics are essentially linear. Competition between catalyst activation and deactivation govern the rapid initial acceleration and slow decay of the CNT number density. The maximum CNT density (i.e., the overall catalyst activity) is limited by gas-phase reactions and catalyst-surface interactions, which collectively exhibit autocatalytic behavior. Thus, we propose a comprehensive picture of CNT population growth which combines both chemical and mechanical cooperation. Our findings are relevant to both bulk and substrate-based CNT synthesis methods and provide general insights into the self-assembly and collective growth of filamentary nanostructures.
Direct Left Atrial Pressure Monitoring in Severe Heart Failure: Long-Term Sensor Performance (2010)

We report the stability, accuracy, and development history of a new left atrial pressure (LAP) sensing system in ambulatory heart failure (HF) patients. A total of 84 patients with advanced HF underwent percutaneous transseptal implantation of the pressure sensor. Quarterly noninvasive calibration by modified Valsalva maneuver was achieved in all patients, and 96.5% of calibration sessions were successful with a reproducibility of 1.2 mmHg. Absolute sensor drift was maximal after 3 months at 4.7 mmHg (95% CI, 3.2-6.2 mmHg) and remained stable through 48 months. LAP was highly correlated with simultaneous pulmonary wedge pressure at 3 and 12 months (r = 0.98, average difference of 0.8 ± 4.0 mmHg). Freedom from device failure was 95% (n = 37) at 2 years and 88% (n = 12) at 4 years. Causes of failure were identified and mitigated with 100% freedom from device failure and less severe anomalies in the last 41 consecutive patients (p = 0.005). Accurate and reliable LAP measurement using a chronic implanted monitoring system is safe and feasible in patients with advanced heart failure.
A Review of Locomotion Systems for Capsule Endoscopy (2015)

Wireless capsule endoscopy for gastrointestinal (GI) tract is a modern technology that has the potential to replace conventional endoscopy techniques. Capsule endoscopy is a pill-shaped device embedded with a camera, a coin battery, and a data transfer. Without a locomotion system, this capsule endoscopy can only passively travel inside the GI tract via natural peristalsis, thus causing several disadvantages such as inability to control and stop, and risk of capsule retention. Therefore, a locomotion system needs to be added to optimize the current capsule endoscopy. This review summarizes the state-of-the-art locomotion methods along with the desired locomotion features such as size, speed, power, and temperature and compares the properties of different methods. In addition, properties and motility mechanisms of the GI tract are described. The main purpose of this review is to understand the features of GI tract and diverse locomotion methods in order to create a future capsule endoscopy compatible with GI tract properties.
The effect of treatment with Campath‐1H in patients with autoimmune cytopenias (2001)

We describe 21 patients with severe and life-threatening autoimmune cytopenias resistant to standard immunosuppression who were treated with the monoclonal antibody Campath-1H. Four patients had autoimmune neutropenia, four had autoimmune haemolytic anaemia, four had pure red cell aplasia, one had immune thrombocytopenia purpura (ITP), three had autoimmune haemolytic anaemia and ITP (Evan's syndrome), three had autoimmune pancytopenia (ITP, autoimmune neutropenia and autoimmune haemolytic anaemia), one had ITP (associated with acquired Glanzmann's disease) and autoimmune neutropenia, and one had ITP and red cell aplasia. Campath-1H was administered at a dose of 10 mg/d as an intravenous infusion for 10 d. Responses were seen in 15 patients, which were sustained in six. Relapse occurred in eight patients after Campath-1H treatment. Patients entering the study later, received cyclosporine after Campath-1H in an attempt to reduce the incidence of relapse. Three patients received a second course of Campath-1H; all responded but later relapsed. Fourteen patients are alive at a median of 12 months (range 4-61) after Campath-1H. Campath-1H represents an alternative therapeutic option for severe, refractory autoimmune cytopenias.
Dynamic queue length thresholds for shared-memory packet switches (1998)

In shared-memory packet switches, buffer management schemes can improve overall loss performance, as well as fairness, by regulating the sharing of memory among the different output port queues. Of the conventional schemes, static threshold (ST) is simple but does not adapt to changing traffic conditions, while pushout (PO) is highly adaptive but difficult to implement. We propose a novel scheme called dynamic threshold (DT) that combines the simplicity of ST and the adaptivity of PO. The key idea is that the maximum permissible length, for any individual queue at any instant of time, is proportional to the unused buffering in the switch. A queue whose length equals or exceeds the current threshold value may accept no more arrivals. An analysis of the DT algorithm shows that a small amount of buffer space is (intentionally) left unallocated, and that the remaining buffer space becomes equally distributed among the active output queues. We use computer simulation to compare the loss performance of DT, ST, and PO. DT control is shown to be more robust to uncertainties and changes in traffic conditions than ST control.
Use of External Magnetic Fields To Reduce Reaction Times in an Immunoassay Using Micrometer-Sized Paramagnetic Particles as Labels (Magnetoimmunoassay) (2004)

The paper presents a rapid immunoassay system capable of quantifying analyte in complex biological and environmental media. Antibody-coated micrometer-sized paramagnetic particles are used as labels in an assay in which they bind quantitatively with an analyte and capture antibody molecules immobilized on a polyester disk to form a sandwich assay. The assay is performed in a simple reaction vessel, and reactions between labels, analyte, and antibodies are accelerated by positioning magnets alternately above and below the vessel. The bound paramagnetic particles are quantified using a simple flat, spiral, coil located just below the polyester disk. The electronic circuitry associated with the coil uses components that are inexpensive and readily available. The coil has been designed to respond only to particles bound on the disk and not to unbound particles still in the test solution. Unbound particles are pulled away from the disk by the magnet before readings are taken. The use of the reaction vessel with the cardiac markers CRP and CKMB is described. No sample preparation or washing step is used in the assays, and results can be obtained in less than 3 min after introducing the sample into the vessel with sensitivities in the normal clinical range.
Dinucleotide Analogues as Novel Inhibitors of RNA-Dependent RNA Polymerase of Hepatitis C Virus (2003)

ABSTRACT Replication of hepatitis C virus (HCV) RNA is catalyzed by the virally encoded RNA-dependent RNA polymerase NS5B. It is believed that the viral polymerase utilizes a de novo or primer-independent mechanism for initiation of RNA synthesis. Our previous work has shown that dinucleotides were efficient initiation molecules for NS5B in vitro (W. Zhong, E. Ferrari, C. A. Lesburg, D. Maag, S. K. Ghosh, C. E. Cameron, J. Y. Lau, and Z. Hong, J. Virol. 74: 9134-9143, 2000). In this study, we further demonstrated that dinucleotide analogues could serve as inhibitors of de novo initiation of RNA synthesis directed by HCV NS5B. Both mononucleotide- and dinucleotide-initiated RNA syntheses were affected by dinucleotide analogues. The presence of the 5′-phosphate group in the dinucleotide compounds was required for efficient inhibition of de novo initiation. Optimal inhibitory activity also appeared to be dependent on the base-pairing potential between the compounds and the template terminal bases. Because the initiation process is a rate-limiting step in viral RNA replication, inhibitors that interfere with the initiation process will have advantages in suppressing virus replication. The use of dinucleotide analogues as inhibitor molecules to target viral replication initiation represents a novel approach to antiviral interference.
Integration of the Soybean Molecular and Classical Genetic Linkage Groups (1995)

To initiate the process of map integration, we used data from a Glycine max × G. max mapping population that had segregating markers at seven pigmentation ( D1 D2 G 1 L1 T Y9 ), six morphological ( Dt1 E2 Lf1 Ln P1 Pd1 ), and seven isozyme ( Aco4 Enp 1dh1 Mdh Mpi Pgi Pgm1 ) loci (underscoring indicates marker members of the classical map). The parents of this population were two multi‐marker strains, one a near‐isogenic line (NIL) of the cultivar Clark, and the other a NIL of the cultivar Harosoy. Sixty F 2.3 progeny lines were characterized for allelic status at the 20 classical loci, 8 RAPD loci, and 110 RFLP loci; the latter previously mapped in the G. max × G. soja mapping population. The computer program JoinMap was used to pool the linkage data obtained in both populations and to build consensus linkage groups. As a result of these analyses, marker T of classical linkage group 1 (CLG‐1) was assigned to molecular linkage group c2 (MLG‐C2), marker P1 of CLG‐2 to MLG‐k, markers Dt1 and L1 of CLG‐5 to MLG‐1, marker Y9 of CLG‐14 to MLG‐e, and marker Mpi of CLG‐18 to MLG‐g. Probes A702 and B219, which had detected two MLG‐bl markers (i.e., A702‐1 and B219‐1 ) in the G. max × G. soja population, detected two different (homoeologous?) markers (i.e., A702‐2 and B219‐4 ) in the Clark × Harosoy population, and these were linked to markers G , D1 , and Pd1 of CLG‐3. Markers Ln and Enp of CLG‐4 were linked to two RFLP markers, but the MLG association was ambiguous because in the G. max × G. soja map one of the RFLP markers mapped to MLG‐f and the other mapped to MLG‐i. With the CLG to MLG assignments made in this study, plus others that can be inferred from the recent literature, about half of the 19 soybean CLGs can now be associated with a corresponding MLG.
EM- and piezo-scavengers: Two useful solutions in highly humanized scenarios toward a &#x0022;greener world&#x0022; (2012)

This contribution discusses different aspects related to EM- and piezo-scavengers from ambient energy that are very promising as power suppliers of the latest evolution of ultra-low power electronic systems. First challenges in designing circuits and systems for microwave ambient energy harvesting are discussed. A design flow, including the RF/Microwave receivers and the base-band conversion blocks is presented. Power densities as low as few μW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> are targeted as realistic sources to be scavenged by a highly efficient multi-band resonant rectenna. Predicted and measured results in stationary regime are presented and the measured converted energy is also assessed. Second, a conventional piezoelectric scavenger is tested as a power source of a nomadic RFID-reader adopted for novel localization system approach. The latter is intended as an example of multidisciplinary (RFID-NFC, ultra-low power systems, short range wireless power transfer, organic technologies and so on), fully autonomous (energy scavenger on board, battery-less), wirelessly empowered (multitude of passive tags is energized by autonomous reader), evolutionary electronic system toward a “greener world”.
A convenient procedure for the preparation of 4(5)-cyanoimidazoles (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA convenient procedure for the preparation of 4(5)-cyanoimidazolesDonald P. Matthews, Jeffrey P. Whitten, and James R. McCarthyCite this: J. Org. Chem. 1986, 51, 16, 3228–3231Publication Date (Print):August 1, 1986Publication History Published online1 May 2002Published inissue 1 August 1986https://pubs.acs.org/doi/10.1021/jo00366a037https://doi.org/10.1021/jo00366a037research-articleACS PublicationsRequest reuse permissionsArticle Views816Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts