gebhart/patrepeval huggingface dataset:
Association of terminal deoxynucleotidyl transferase with Ku (1999)

Terminal deoxynucleotidyl transferase (TdT) catalyzes the addition of nucleotides at the junctions of rearranging Ig and T cell receptor gene segments, thereby generating antigen receptor diversity. Ku is a heterodimeric protein composed of 70- and 86-kDa subunits that binds DNA ends and is required for V(D)J recombination and DNA double-strand break (DSB) repair. We provide evidence for a direct interaction between TdT and Ku proteins. Studies with a baculovirus expression system show that TdT can interact specifically with each of the Ku subunits and with the heterodimer. The interaction between Ku and TdT is also observed in pre-T cells with endogenously expressed proteins. The protein-protein interaction is DNA independent and occurs at physiological salt concentrations. Deletion mutagenesis experiments reveal that the N-terminal region of TdT (131 amino acids) is essential for interaction with the Ku heterodimer. This region, although not important for TdT polymerization activity, contains a BRCA1 C-terminal domain that has been shown to mediate interactions of proteins involved in DNA repair. The induction of DSBs in Cos-7 cells transfected with a human TdT expression construct resulted in the appearance of discrete nuclear foci in which TdT and Ku colocalize. The physical association of TdT with Ku suggests a possible mechanism by which TdT is recruited to the sites of DSBs such as V(D)J recombination intermediates.
Cognitive Radio Formulation and Implementation (2006)

This paper approaches cognition on the physical and MAC layers by defining a common language of "knobs" and "meters" to discuss adaptation and learning. Cognitive radio merges artificial intelligence and software defined radios (SDR). It requires a simple language for communicating between these two levels. We define a method for doing this. We also discuss a genetic algorithm approach to perform intelligent radio adaptation, using the GNU radio platform as an example. We provide both conceptual and practical implementation details of a cognitive radio acting at the physical and MAC layers. Results presented show the promise for the genetic algorithm adaptation within the multi-objective optimization environment of the cognitive radio
Enhancement of Varicella-Zoster-Specific Immune Responses in the Elderly by Boosting with Varicella Vaccine (1984)

Journal Article Enhancement of Varicella-Zoster-Specific Immune Responses in the Elderly by Boosting with Varicella Vaccine Get access Rosemarie Berger, Rosemarie Berger Department of Microbiology and Immunology, University Children's Hospital, Basel, Switzerland Search for other works by this author on: Oxford Academic PubMed Google Scholar Doris Luescher, Doris Luescher Department of Microbiology and Immunology, University Children's Hospital, Basel, Switzerland Search for other works by this author on: Oxford Academic PubMed Google Scholar Max Just Max Just Department of Microbiology and Immunology, University Children's Hospital, Basel, Switzerland Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 149, Issue 4, April 1984, Page 647, https://doi.org/10.1093/infdis/149.4.647 Published: 01 April 1984
Direct Observation of Cavitation and Fibrillation in a Probe Tack Experiment on Model Acrylic Pressure-Sensitive-Adhesives (1999)

Abstract The adhesion mechanisms of two acrylic Pressure-Sensitive-Adhesives on a stainless steel probe are investigated with a custom-designed probe tack apparatus. Our setup allows the simultaneous acquisition of a nominal stress and strain curve, and the observation of the adhesive film from underneath the transparent substrate. The temperature was varied in the range -20°C to 50°C and the debonding rate in the range 1–10000 μm/s. For all conditions we observed, upon debonding, the formation of cavities at or near the interface between the probe and the film. These cavities initially grew predominantly in the plane of the film but, at higher values of nominal strain, the walls between the cavities were stretched in the direction normal to the plane of the ifim to become a fibrillar structure. The transition from a cavitated structure to a fibrillar one was only found within a time-temperature window of rheological properties of the adhesive, while the adhesion energy was found to be mainly related to the elongational properties of the adhesive. The maximum tensile stress observed in the probe tack experiment was directly related to the appearance of the cavities and showed a good correlation with the shear modulus of the adhesive, while the adhesion energy was found to be mainly related to the elongational properties of the adhesive. The presence of 2% acrylic acid as a comonomer had a negligible effect on the maximum stress but a very important one on the formation of a fibrillar structure and on the locus of failure.
Single-camera sequential-scan-based polarization-sensitive SDOCT for retinal imaging (2009)

A single-camera, high-speed, polarization-sensitive, spectral-domain optical-coherence-tomography system was developed to measure the polarization properties of the in vivo human retina. A novel phase-unwrapping method in birefringent media is described to extract the total reflectivity, accumulative retardance, and fast-axis orientation from a specially designed sequence of polarization states incident on the sample. A quarter-wave plate was employed to test the performance of the system. The average error and standard deviation of retardation measurements were 3.2 degrees and 2.3 degrees , respectively, and of the fast-axis orientation 1.2 degrees and 0.7 degrees over the range of 0 degrees -180 degrees . The depolarization properties of the retinal pigment epithelium were clearly observed in both retardance and fast-axis orientation image. A normalized standard deviation of the retardance and of the fast-axis orientation is introduced to segment the polarization-scrambling layer of the retinal pigment epithelium.
Boronated starburst dendrimer-monoclonal antibody immunoconjugates: Evaluation as a potential delivery system for neutron capture therapy (1994)

Boron neutron capture therapy (BNCT) is based on the nuclear capture reaction that occurs when boron-10, a stable isotope, is irradiated with low-energy or thermal neutrons (< or = 0.025 eV) to yield high LET alpha particles and recoiling 7Li nuclei [10B + nth-->[11B]-->4He(alpha) + 7Li + 2.39 MeV]. Approximately 10(9) boron-10 atoms must be delivered to each target cell in order to sustain a lethal 10B(n,alpha)7Li reaction. If MoAbs are to be used for targeting boron-10, then it is essential that they recognize a surface membrane epitope that is highly expressed on tumor cells and that a large number of boron-10 atoms be attached to each antibody molecule. In order to heavily boronate MoAbs, we have utilized starburst dendrimers (SD), which are precise, spherical macromolecules composed of repetitive poly(amidoamino) groups. Second- and fourth-generation dendrimers, having 12 and 48 reactive terminal amino groups and molecular weights of 2414 and 10,632 Da, respectively, were boronated using an isocyanato polyhedral borane, Na(CH3)3NB10H8NCO. The boronated starburst dendrimers (BSD), in turn, were derivatized with m-maleimidobenzoyl N-hydroxysulfosuccinimide ester (sulfo-MBS). The MoAbIB16-6, which is directed against the murine B16 melanoma, was derivatized with N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP). The MBS-derivatized BSD and SPDP-derivatized MoAb were reacted to yield stable immunoconjugates.(ABSTRACT TRUNCATED AT 250 WORDS)
Use of (CAT)5as a DNA Fingerprinting Probe for Fungi (1994)

A new DNA fingerprinting probe, (CAT)s, was evaluated for its ability to detect variation in higher fungi. The probe, which apparently detects variable number tandem repeat (VNTR) loci, was shown to be highly sensitive to genetic variation. The DNA of each of 30 single-basidiospore progeny of a dikaryon of Hererobasidion onnosum exhibited a unique fingerprint after PstI restriction, electrophoresis, and in-gel hybridization with the oligonucleotide. Twelve of 28 scored bands were heterozygous in the parent dikaryon. Six bands segregated in the expected 1:1 ratio, and the remaining six exhibited skewed segregation ratios. Two new bands were observed among the progeny but not the parent dikaryon, suggesting a high mutation rate (>0.3%), consistent with other reported VNTR loci [...]
Determination of electromagnetic properties of low-pressure electrodeless inductive discharges (1990)

A method is described for the determination of current, power, electrical conductivity and the electric and magnetic field strengths in electrodeless inductive low-pressure gas discharges. A transformer model for discharge and induction coil is derived from Maxwell's equations. The method is applied to a low-pressure argon-mercury discharge excited between 3 and 8 MHz.
Mitochondrial creatine kinase: a major constituent of pathological inclusions seen in mitochondrial myopathies. (1994)

Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial encephalomyopathies. Characteristic for the abnormal mitochondria is the occurrence of highly ordered crystalline inclusions. Immuno-electron microscopy revealed that these inclusions react heavily with specific antibodies against mitochondrial creatine kinase (Mi-CK). Image processing of selected crystalline inclusions, sectioned along the crystallographic b, c planes, resulted in an averaged picture displaying an arrangement of regular, square-shaped particles with a central cavity. The overall appearance, dimensions, and symmetry of these building blocks are very reminiscent of single isolated Mi-CK octamers. Taking these findings together, it is concluded that Mi-CK octamers indeed represent the major, if not the only, component of these mitochondrial inclusions.
CpG Island Methylation of DNA Damage Response Genes in Advanced Ovarian Cancer (2005)

We have determined the methylation frequencies of 24 CpG islands of genes associated with DNA damage responses or with ovarian cancer in 106 stage III/IV epithelial ovarian tumors. We have analyzed this data for whether there is evidence of a CpG island methylator phenotype or associations of CpG island methylation with response to chemotherapy in advanced ovarian cancer. Frequent methylation was observed for OPCML, DCR1, RASSF1A, HIC1, BRCA1, and MINT25 (33.3%, 30.7%, 26.4%, 17.3%, 12.3%, and 12.0%, respectively), whereas no methylation was observed for APAF-1, DAPK, FANCF, FAS, P14, P21, P73, SOCS-3, and SURVIVIN. The remaining genes showed only a low frequency of methylation, <10%. Unsupervised gene shaving identified a nonrandom pattern of methylation for OPCML, DCR1, RASSF1A, MINT25, HIC1, and SFRP1, supporting the concept of concordant methylation of these genes in ovarian cancer. Methylation of at least one of the group of genes involved in DNA repair/drug detoxification (BRCA1, GSTP1, and MGMT) was associated with improved response to chemotherapy (P = 0.013). We have examined the frequency of a polymorphism in the DNA methyltransferase gene DNMT3b6, which has been previously reported to affect gene transcription and cancer risk. The genetic polymorphism in the DNMT3b6 gene promoter (at position -149) is not significantly associated with the concordant methylation observed, but is weakly associated with the overall frequency of methylation at the genes examined (P = 0.04, n = 56). This supports the hypothesis that genetic factors affecting function of DNMT genes may underlie the propensity of tumors to acquire aberrant CpG island methylation.
Technology in decline: a search for useful concepts (1992)

Until late in the nineteenth century, madder was the most popular natural red dye. Holland was the largest and best-known supplier. As early as the fourteenth and fifteenth centuries, the province of Zeeland and adjoining parts of the provinces of South Holland and Brabant developed into important producers. In the course of the seventeenth century these areas even succeeded in acquiring a monopoly position. Early in the nineteenth century, however, this position came under attack because France had gone over to industrial production methods from around 1800, whereas Holland continued to produce with craft technologies. After 1820, as a result, a period of stagnation and decay set in. The fate of the Dutch madder industry would have been completely sealed if the production capacity of the French factories had been sufficiently large to satisfy the great increase in demand. Consequently, in Holland, after 1845, a process of revival based upon the French manufacturing methods began slowly and hesitatingly. This process started too late and was not persevered with sufficiently to regain lost markets. The Dutch producers remained strongly attached to their own out-dated, craft methods of production.
Acquiring and Revising Preferences in a Critique-Based Mobile Recommender System (2007)

Many e-commerce Web sites offer numerous services, so a product search could return an overwhelming set of options. Without system support, filtering irrelevant products, comparing alternatives, and selecting the best option can be difficult or impossible - especially for users connecting to the Web through a mobile device. Few Web-based recommender systems have been designed for mobile users. A critique-based recommendation methodology aids the acquisition and revision of user preferences in a mobile recommender system. We designed a product recommendation methodology and implemented it in MobyRek, a mobile-phone recommender system that helps users search for travel products. MobyRek supports limited asking and answering of questions and is based mostly on critiques.
Evidence for the Presence of an Alternative Glucose Transport System in Clostridium beijerinckii NCIMB 8052 and the Solvent-Hyperproducing Mutant BA101 (2005)

The effects of substrate analogs and energy inhibitors on glucose uptake and phosphorylation by Clostridium beijerinckii provide evidence for the operation of two uptake systems: a previously characterized phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a non-PTS system probably energized by the transmembrane proton gradient. In both wild-type C. beijerinckii NCIMB 8052 and the butanol-hyperproducing mutant BA101, PTS activity declined at the end of exponential growth, while glucokinase activity increased in the later stages of fermentation. The non-PTS uptake system, together with enhanced glucokinase activity, may provide an explanation for the ability of the mutant to utilize glucose more effectively during fermentation despite the fact that it is partially defective in PTS activity.
Terajets produced by dielectric cuboids (2014)

The capability of generating terajets using three-dimensional (3D) dielectric cuboids working at terahertz (THZ) frequencies (as analogues of nanojets in the infrared band) is introduced and studied numerically. The focusing performance of the terajets is evaluated in terms of the transversal full width at half maximum (FWHM) along x- and y-directions using different refractive indices for a 3D dielectric cuboid with a fixed geometry, obtaining a quasi-symmetric terajet with a subwavelength resolution of ∼0.46λ0 when the refractive index is n = 1.41. Moreover, the backscattering enhancement produced when metal particles are introduced in the terajet region is demonstrated for a 3D dielectric cuboid and compared with its two-dimensional (2D) counterpart. The results of the jet generated for the 3D case are experimentally validated at sub-THZ waves, demonstrating the ability to produce terajets using 3D cuboids.
Regulation of amino acid-sensitive TOR signaling by leucine analogues in adipocytes (2000)

In adipocytes, amino acids stimulate the target of rapamycin (TOR) signaling pathway leading to phosphorylation of the translational repressor, eIF-4E binding protein-I (4E-BP1), and ribosomal protein S6. L-leucine is the primary mediator of these effects. The structure-activity relationships of a putative L-leucine recognition site in adipocytes (LeuR(A)) that regulates TOR activity were analyzed by examining the effects of leucine analogues on the rapamycin-sensitive phosphorylation of the translational repressor, eIF-4E binding protein-I (4E-BP1), an index of TOR activity. Several amino acids that are structurally related to leucine strongly stimulated 4E-BP1 phosphorylation at concentrations greater than the EC(50) value for leucine. The order of potency was leucine > norleucine > threo-L-beta-hydroxyleucine approximately Ile > Met approximately Val. Other structural analogues of leucine, such as H-alpha-methyl-D/L-leucine, S-(-)-2-amino-4-pentenoic acid, and 3-amino-4-methylpentanoic acid, possessed only weak agonist activity. However, other leucine-related compounds that are known agonists, antagonists, or ligands of other leucine binding/recognition sites did not affect 4E-BP1 phosphorylation. We conclude from the data that small lipophilic modifications of the leucine R group and alpha-hydrogen may be tolerated for agonist activity; however, leucine analogues with a modified amino group, a modified carboxylic group, charged R groups, or bulkier aliphatic R groups do not seem to possess significant agonist activity. Furthermore, the leucine recognition site that regulates TOR signaling in adipocytes appears to be different from the following: (1) a leucine receptor that regulates macroautophagy in liver, (2) a leucine recognition site that regulates TOR signaling in H4IIE hepatocytes, (3) leucyl tRNA or leucyl tRNA synthetase, (4) the gabapentin-sensitive leucine transaminase, or (5) the system L-amino acid transporter.
Nucleotides and Nucleic Acids: Oligo‐ and Polynucleotides (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
KOC (K Homology Domain Containing Protein Overexpressed in Cancer) (2005)

KOC (K homology domain containing protein overexpressed in cancer) is a novel oncofetal RNA-binding protein highly expressed in pancreatic carcinomas. Recently, Corixa Corporation developed a monoclonal antibody specific for KOC that can be used with standard immunohistochemical techniques. The purposes of this study were 1) to assess KOC mRNA expression in pancreatic carcinoma, 2) to determine the pattern of KOC immunoexpression among benign, borderline, and malignant pancreatic epithelial lesions, and 3) to evaluate the utility of the KOC antibody in distinguishing between these entities. mRNA was isolated from fresh pancreatic tissues (19 carcinomas, 2 normal pancreas, 1 chronic pancreatitis) and amplified using standard RT-PCR techniques. Fifteen of 19 (79%) carcinomas overexpressed KOC mRNA relative to non-neoplastic tissue samples and expression increased progressively with tumor stage: the mean copy number of KOC mRNA transcripts was 1.5, 11.1, 31, and 28 for stage I, II, III, and IV carcinomas, respectively, compared with 0.9 and 1 for normal pancreatic tissue and chronic pancreatitis, respectively. Immunostains using the KOC antibody were performed on 50 surgical resection specimens (38 invasive adenocarcinomas, 3 intraductal papillary-mucinous neoplasms, 2 mucinous cystic neoplasms, 7 chronic pancreatitis). KOC staining was present in 37 of 38 (97%) carcinomas: the staining reaction was moderate or strong in 36 of 38 (94%) and present in >50% of the tumor cells in 35 of 38 (92%) cases. Severe dysplasia of the ductal epithelium, present in 19 foci of intraductal papillary mucinous carcinoma, mucinous cystadenocarcinoma, and grade 3 pancreatic intraepithelial neoplasia (PanIN3) showed strong or moderate staining in 15 (79%) cases, whereas foci of mild and moderate dysplasia (intraductal papillary-mucinous neoplasms and mucinous cystic neoplasms with adenoma and/or moderate dysplasia, PanIN1, and PanIN2) were uniformly negative for this marker in 25 and 22 cases, respectively. In the normal pancreas, weak background staining of acini was present in 12 of 50 (24%) cases but was easily distinguishable from the type of staining identified in neoplastic epithelium, and benign ducts and ductules were negative in all cases. Four of 38 (11%) foci of chronic pancreatitis, present in the 7 resections performed for chronic pancreatitis as well as 31 foci of peritumoral chronic pancreatitis, showed weak staining in <10% of the ductules. We conclude that KOC is a sensitive and specific marker for carcinomas and high-grade dysplastic lesions of the pancreatic ductal epithelium. Therefore, immunostains directed against KOC may be of diagnostic utility in the evaluation of pancreatic lesions, particularly when biopsy material is limited.
First‐trimester screening for trisomy 21 by free beta‐human chorionic gonadotropin and pregnancy‐associated plasma protein‐A: impact of maternal and pregnancy characteristics (2008)

In first-trimester biochemical screening for trisomy 21 it is essential to adjust the measured values of free beta-hCG and PAPP-A for maternal and pregnancy characteristics.
Technology's impact on online resumes (2002)

Technical communicators understand that converting a paper-based manual to the online world does not merely require "dumping" the document. Likewise, converting a paper-based resume to an electronic one is not simply a function of "dumping" the document online. The technology impacts purpose and audience, and therefore, influences the ways in which to design the document. This paper focuses on some of the differences between paper-based and online resumes. It shows that the "body of knowledge" under which technical communicators normally operate does not necessarily apply in the realm of the online resume world.
Therapeutic Potential and Strategies for Inhibiting Tumor Necrosis Factor-α (1999)

ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTTherapeutic Potential and Strategies for Inhibiting Tumor Necrosis Factor-αRobert C. Newton and Carl P. DeciccoView Author Information Departments of Inflammatory Diseases Research and Chemical and Physical Sciences, The DuPont Pharmaceuticals Company, Experimental Station, P.O. Box 80500, Wilmington, Delaware 19880-0500 Cite this: J. Med. Chem. 1999, 42, 13, 2295–2314Publication Date (Web):July 1, 1999Publication History Received28 September 1998Published online1 July 1999Published inissue 1 July 1999https://pubs.acs.org/doi/10.1021/jm980541nhttps://doi.org/10.1021/jm980541nreview-articleACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views971Altmetric-Citations161LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Cell signaling,Genetics,Inhibitors,Peptides and proteins,Receptors Get e-Alerts