gebhart/patrepeval huggingface dataset:
Molecular diversity of Ca2+ channel β subunits (1994)

Introduction Voltage-gated Ca2+ channels have been extensively characterized in terms of their pharmacological, electrophysiological, biochemical, and molecular biological properties. Calcium channels are multisubunit complexes composed of an ion-conducting subunit, a ,, and smaller accessory subunits. Molecular cloning studies have concentrated on the a , subunit because it appears to determine the pharmacological and electrophysiological properties of the channel. In fact, expression of cloned a, subunits from skeletal muscle and cardiac muscle displayed I>-type CaZc-channel activity, whereas other distinct, yet related, a I subunits display N-type activity [l-31. So far, cDNAs from six a , genes have been cloned. Alignment of the deduced amino acid sequences reveals that these a,s are members of a superfamily that can be subdivided into I,-type and non-L-type Ca2+ channels. Despite experiments showing that the accessory subunits are capable of modulating the properties of a , subunits [2,4], cloning of these subunits has until recently received little attention. In this report, we summarize our cloning of Psubunit cDNAs derived from four distinct genes and present new data on alternatively spliced forms. We also summarize our expression studies using a representative member of each of these P subclasses.
Epidural adhesions after chymopapain chemonucleolysis. (1989)

Epidural adhesions were discovered in seven of nine patients undergoing surgery for failed chymopapain chemonucleolysis. Clinically the adhesions were very vascular, prolific and adherent to the nerve root, dura and the porieties. The histological composition was similar to the normal tissues of the epidural space from the post-mortem controls and also to adhesions discovered at surgery in a non-injected patient, except that there was an increase in fibrous tissue, which was poorly cellular. There was no evidence of an inflammatory infiltrate. Such adhesions may partly explain the failure of chemonucleolysis and prejudice the success of surgery by potentiating further adhesion formation.
Technique for Achievement and Maintenance of Reduction for Severe Spondylolisthesis Using Spinous Process Traction Wiring and External Fixation of the Pelvis (1985)

A technique is described for achievement and maintenance of reduction for severe spondylolisthesis in conjunction with reconstructive surgery. Spinous-process traction wires exert a posterior and cephalad force on the lumbar spine, while Hoffman pins anchored to the ilium rotate the sacrum in an anterior and caudad direction. Once maximum reduction is achieved, the patient is kept supine for 3 months with pins and traction wires incorporated in a plaster cast. At follow-up, two patients demonstrated excellent correction of slip angle and a solid fusion.
Renal hypomagnesaemia in human diabetes mellitus: its relation to glucose homeostasis (1982)

Interrelations between magnesium and glucose metabolism were studied in 215 insulin-treated diabetic out-patients aged 7-70 years. All had normal serum creatinine concentrations (below 115 mumol/l) and none had other diseases or received drugs known to interfere with mineral metabolism. A definite hypomagnesaemia (less than normal mean - 2 SD) and hypermagnesiuria (greater than normal mean + 2 SD) occurred in 38.6% and 55% of the patients. In the presence of hypermagnesiuria the serum magnesium concentration was inversely correlated to the urinary magnesium excretion rate (R = -0.23, P less than 0.02). Serum magnesium correlated inversely with both fasting blood glucose (R = -0.32, P less than 0.001) and the urinary glucose excretion rate (R = -0.22, P less than 0.005). The urinary magnesium excretion rate correlated directly with the same variables (R = 0.27, P less than 0.001 and R = 0.58, P less than 0.001, respectively). These data indicate that the net tubular reabsorption of magnesium is decreased in diabetic patients in presence of hyperglycaemia, leading to hypermagnesiuria and hypomagnesaemia.
Construction of Glyco-Clusters by Self-Organization of Site-Specifically Glycosylated Oligonucleotides and Their Cooperative Amplification of Lectin-Recognition (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTConstruction of Glyco-Clusters by Self-Organization of Site-Specifically Glycosylated Oligonucleotides and Their Cooperative Amplification of Lectin-RecognitionKazunori Matsuura, Miki Hibino, Yoshinao Yamada, and Kazukiyo KobayashiView Author Information Department of Molecular Design Graduate School of Engineering Nagoya University, Chikusa, Nagoya 464-8603, Japan Cite this: J. Am. Chem. Soc. 2001, 123, 2, 357–358Publication Date (Web):December 16, 2000Publication History Received2 June 2000Published online16 December 2000Published inissue 1 January 2001https://pubs.acs.org/doi/10.1021/ja001945jhttps://doi.org/10.1021/ja001945jrapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views854Altmetric-Citations95LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biopolymers,Conjugate acid-base pairs,Genetics,Peptides and proteins,Self organization Get e-Alerts
Progressive Familial Intrahepatic Cholestasis: A Rare Cause of Cirrhosis in Young Adult Patients (2015)

Hepatic cirrhosis is an important cause of morbidity and mortality. An unusual case of cirrhosis and portal hypertension in an 18-year-old patient secondary to Progressive Intrahepatic Cholestasis is discussed. The clinical and biochemical findings are discussed and a clinical approach to determining the underlying etiology of cirrhosis is outlined. Significant complications of portal hypertension include ascites, spontaneous bacterial peritonitis, hepatorenal syndrome, varices, and hepatic encephalopathy. A clinical approach to these complications of cirrhosis is presented. Progressive Familial Intrahepatic Cholestasis (PFIC) is a rare congenital metabolic abnormality. There are 3 subtypes and Type 3 PFIC commonly presents in late adolescence and early adulthood. Clinical and laboratory findings as well as management for the condition are described.
Interleukin-11 (IL-11) and IL-9 counteract the inhibitory activity of transforming growth factor beta 3 (TGF-beta 3) on human primitive hematopoietic progenitor cells. (1995)

These data confirm that TGF-beta 3 exerts its suppressive effect on hematopoietic progenitor cells according to the differentiation state of the target cells and the presence of other cytokines interacting with the cells. The permissive growth factors IL-11 and IL-9 seem to be able to partially counteract the negative regulation of TGF-beta 3.
A comprehensive review of techniques for biofunctionalization of titanium (2011)

A number of surface modification techniques using immobilization of biofunctional molecules of Titanium (Ti) for dental implants as well as surface properties of Ti and Ti alloys have been developed. The method using passive surface oxide film on titanium takes advantage of the fact that the surface film on Ti consists mainly of amorphous or low-crystalline and non-stoichiometric TiO(2). In another method, the reconstruction of passive films, calcium phosphate naturally forms on Ti and its alloys, which is characteristic of Ti. A third method uses the surface active hydroxyl group. The oxide surface immediately reacts with water molecules and hydroxyl groups are formed. The hydroxyl groups dissociate in aqueous solutions and show acidic and basic properties. Several additional methods are also possible, including surface modification techniques, immobilization of poly(ethylene glycol), and immobilization of biomolecules such as bone morphogenetic protein, peptide, collagen, hydrogel, and gelatin.
Spinel Anodes for Lithium‐Ion Batteries (1994)

Anodes of , , and with a spinel‐type structure have been evaluated in room‐temperature lithium cells. The cathodes that were selected for this study were the stabilized spinels, and , and layered . The electrochemical data demonstrated that Li+ ions will shuttle between two transition‐metal host structures (anode and cathode) at a reasonably high voltage with a concomitant change in the oxidation state of the transition metal cations so that the Li+ ions do not reduce to the metallic state at the anode during charge. These cells reduce the safety hazards associated with cells containing metallic‐lithium, lithium‐alloy, and lithium‐carbon anodes.
Five- and Six-Membered Conformationally Locked 2‘,4‘-Carbocyclicribo-Thymidines:  Synthesis, Structure, and Biochemical Studies (2007)

Two unusual reactions involving the 5-hexenyl or the 6-heptenyl radical cyclization of a distant double bond at C4' and the radical center at C2' of the ribofuranose ring of thymidine have been used as key steps to synthesize North-type conformationally constrained cis-fused bicyclic five-membered and six-membered carbocyclic analogues of LNA (carbocyclic-LNA-T) and ENA (carbocyclic-ENA-T) in high yields. Their structures have been confirmed unambiguously by long range 1H-13C NMR correlation (HMBC), TOCSY, COSY, and NOE experiments. The carbocyclic-LNA-T and carbocyclic-ENA-T were subsequently incorporated into the antisense oligonucleotides (AONs) to show that they enhance the Tm of the modified AON/RNA heteroduplexes by 3.5-5 degrees C and 1.5 degrees C/modification for carbocyclic-LNA-T and carbocyclic-ENA-T, respectively. Whereas the relative RNase H cleavage rates with carbocyclic-LNA-T, carbocyclic-ENA-T, aza-ENA-T, and LNA-T modified AON/RNA duplexes were found to be very similar to that of the native counterpart, irrespective of the type and the site modification in the AON strand, a single incorporation of carbocyclic-LNA and carbocyclic-ENA into AONs leads to very much more enhanced nuclease stability in the blood serum (stable >48 h) as compared to that of the native (fully degraded <3 h) and the LNA-modified AONs (fully degraded <9 h) and aza-ENA ( approximately 85% stable in 48 h). Clearly, remarkably enhanced lifetimes of these carbocyclic-modified AONs in the blood serum may produce the highly desired pharmacokinetic properties because of their unique stability and consequently a net reduction of the required dosage. This unique quality as well as their efficient use as the AON in the RNase H-promoted cleavage of the target RNA makes our carbocyclic-LNA and carbocyclic-ENA modifications excellent candidates as potential antisense therapeutic agents.
High-efficiency (7.2%) flexible dye-sensitized solar cells with Ti-metal substrate for nanocrystalline-TiO2photoanode (2006)

High-efficiency flexible dye-sensitized solar cells were fabricated with a Ti-metal foil substrate for photo anode and using a Pt-electrodeposited counter electrode on ITO/polyethylene naphthalate (ITO/PEN); these devices were characterized by incident photon-to-current efficiency (IPCE), optical transmittance and electrical impedance spectroscopy.
Biomarkers for Prostate Cancer Detection (2007)

The process of discovering novel biomarkers to replace or augment the existing best marker, prostate specific antigen, requires standardized phases of evaluation and validation. Several biomarkers are currently on the cusp of initial validation studies.
YfcX Enables Medium-Chain-Length Poly(3-Hydroxyalkanoate) Formation from Fatty Acids in Recombinant Escherichia coli fadB Strains (2002)

Expression of Escherichia coli open reading frame yfcX is shown to be required for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) formation from fatty acids in an E. coli fadB mutant. The open reading frame encodes a protein, YfcX, with significant similarity to the large subunit of multifunctional beta-oxidation enzymes. E. coli fadB strains modified to contain an inactivated copy of yfcX and to express a medium-chain-length synthase are unable to form PHA(MCL)s when grown in the presence of fatty acids. Plasmid-based expression of yfcX in the FadB(-) YfcX(-) PhaC(+) strain restores polymer formation. YfcX is shown to be a multifunctional enzyme that minimally encodes hydratase and dehydrogenase activities. The gene encoding YfcX is located downstream from yfcY, a gene encoding thiolase activity. Results of insertional inactivation studies and enzyme activity analyses suggest a role for yfcX in PHA monomer unit formation in recombinant E. coli fadB mutant strains. Further studies are required to determine the natural role of YfcX in the metabolism of E. coli.
[Effects of 5 derivatives of 3-azabicyclo [3,3,1]nonanes on isolated guinea pig ileum myenteric plexus-longitudinal muscle]. (1985)

Five derivatives of 3-azabicyclo [3,3,1] nonanes all inhibited the contaction of guinea pig ileum myenteric plexus-longitudinal muscle(GPIML)induced by electrical stimulation.Except P7520, the inhibitions of all compounds were more potent than that of morphine. The relatively specific mu receptor antagonist naloxone completely reversed their inhibitory effects.The concentration-response curves of these compounds were parallel to that of morphine. There were good correlations between inhibitory potencies on GPIML and analgesic potencies in mouse hot plate test, mouse writhing test and rat tail-flick test,and binding affinities for the opiate receptors of mouse brain for these 5 compounds. These results supported the view that the analgesic actions of these compounds were mainly related to mu receptors. The inhibitory potency of P7521(IC50 85 pM)on GPIML was 4134 times that of morphine(IC50 0.35 microM), indicating that P7521 had a high affinity for mu receptors in GPIML.
Sustained delivery of paclitaxel using thermogelling poly(PEG/PPG/PCL urethane)s for enhanced toxicity against cancer cells (2012)

A multiblock poly(ether ester urethane)s comprising poly(ε-caprolactone), poly(ethylene glycol), and poly(propylene glycol) segments was synthesized. The aqueous copolymer solution exhibited thermogelling behavior at a critical gelation concentration of 3 wt %. The chemical structure and molecular characteristic of the copolymers were studied by gel permeation chromatography, NMR, and fourier transform infrared spectroscopy (FTIR). Rheological characterizations on the thermogel were carried out. Drug release studies using paclitaxel showed that sustained drug release of more than 2 weeks can be achieved with this system. The paclitaxel-loaded gels showed efficiency in the control of the growth of HeLa cells when compared with the paclitaxel dissolved in solution or paclitaxel encapsulated in Pluronics F127. The results demonstrated that the copolymers could be potentially used in chemotherapeutic applications.
20Gb/s PAM-4 Transmission from 35 to 90°C by modulating a Silicon Ring Resonator Modulator with 2Vpp (2015)

A PAM-4 transmission is established by modulating a Si-RRM with a 2Vpp driving voltage. A 20Gb/s bit-rate is achieved with an 8GHz bandwidth, and maintained while the Si-chip temperature is varied from 35 to 90°C.
Facile Syntheses of Titanium(II), Tin(II), and Vanadium(II) Porphyrin Complexes through Homogeneous Reduction. Reactivity of trans-(TTP)TiL2 (L = THF, t-BuNC) (1998)

Facile syntheses of the meso-tetra-p-tolylporphyrin (TTP) complexes trans-(TTP)Ti(THF)(2) (1), (TTP)Sn (2), and trans-(TTP)V(THF)(2) (3) are achieved through homogeneous reduction of high-valent precursors using NaBEt(3)H. The composition of the new compound trans-(TTP)Ti(THF)(2) was determined by spectroscopic and chemical characterization. Ligand displacement reactions of trans-(TTP)Ti(THF)(2) with t-BuNC produced a new Ti(II) complex, trans-(TTP)Ti(t-BuNC)(2). The ligand-binding preference of (TTP)Ti(II)L(n)() (n = 1, 2) is picoline approximately pyridine > t-BuNC > PhC&tbd1;CPh > EtC&tbd1;CEt > THF.
Expression of epidermal growth factor receptor correlates with disease relapse and progression to androgen-independence in human prostate cancer. (2002)

EGFR expression increases during the natural history of prostate cancer. Correlation with disease progression and hormone-refractory disease suggests that EGFR-targeted drugs could be of therapeutic relevance in prostate cancer.
Tissue-Infiltrating Neutrophils Constitute the Major In Vivo Source of Angiogenesis-Inducing MMP-9 in the Tumor Microenvironment (2014)

According to established notion, one of the major angiogenesis-inducing factors, pro-matrix metalloproteinase-9 (proMMP-9), is supplied to the tumor microenvironment by tumor-associated macrophages (TAMs). Accumulated evidence, however, indicates that tumor-associated neutrophils (TANs) are also critically important for proMMP-9 delivery, especially at early stages of tumor development. To clarify how much angiogenic proMMP-9 is actually contributed by TAMs and TANs, we quantitatively evaluated TAMs and TANs from different tumor types, including human xenografts and syngeneic murine tumors grown in wild-type and Mmp9-knockout mice. Whereas host MMP-9 competence was required for full angiogenic potential of both normal and tumor-associated leukocytes, direct comparisons of neutrophils versus macrophages and TANs versus TAMs demonstrated that macrophages and TAMs secrete 40- to 50-fold less proMMP-9 than the same numbers of neutrophils or TANs. Correspondingly, the levels of MMP-9-mediated in vivo angiogenesis induced by neutrophils and TANs substantially exceeded those induced by macrophages and TAMs. MMP-9-delivering TANs were also required for development of metastasis-supporting intratumoral vasculature, characterized by ≥ 11-μm size lumens and partial coverage with stabilizing pericytes. Importantly, MMP-9-producing TAMs exhibit M2-skewed phenotype but do not express tissue inhibitor of metalloproteinases-1 (TIMP-1), a novel characteristic allowing them to secrete TIMP-1-free, neutrophil-like MMP-9 zymogen unencumbered by its natural inhibitor. Together, our findings support the notion whereby TANs, capable of immediate release of their pre-stored cargo, are the major contributors of highly angiogenic MMP-9, whereas tumor-influxing precursors of macrophages require time to differentiate, polarize into M2-skewed TAMs, shut down their TIMP-1 expression, and only then, initiate relatively low-level production of TIMP-free MMP-9 zymogen.
Advanced Glycation End Products Induce Obesity and Hepatosteatosis in CD-1 Wild-Type Mice (2016)

AGEs are a heterogeneous group of molecules formed from the nonenzymatic reaction of reducing sugars with free amino groups of proteins, lipids, and/or nucleic acids. AGEs have been shown to play a role in various conditions including cardiovascular disease and diabetes. In this study, we hypothesized that AGEs play a role in the "multiple hit hypothesis" of nonalcoholic fatty liver disease (NAFLD) and contribute to the pathogenesis of hepatosteatosis. We measured the effects of various mouse chows containing high or low AGE in the presence of high or low fat content on mouse weight and epididymal fat pads. We also measured the effects of these chows on the inflammatory response by measuring cytokine levels and myeloperoxidase activity levels on liver supernatants. We observed significant differences in weight gain and epididymal fat pad weights in the high AGE-high fat (HAGE-HF) versus the other groups. Leptin, TNF-α, IL-6, and myeloperoxidase (MPO) levels were significantly higher in the HAGE-HF group. We conclude that a diet containing high AGEs in the presence of high fat induces weight gain and hepatosteatosis in CD-1 mice. This may represent a model to study the role of AGEs in the pathogenesis of hepatosteatosis and steatohepatitis.
Incorporation of correlated structural images in PET image reconstruction (1994)

Reports on a new method in which spatially correlated magnetic resonance (MR) or X-ray computed tomography (CT) images are employed as a source of prior information in the Bayesian reconstruction of positron emission tomography (PET) images. This new method incorporates the correlated structural images as anatomic templates which can be used for extracting information about boundaries that separate regions exhibiting different tissue characteristics. In order to avoid the possible introduction of artifacts caused by discrepancies between functional and anatomic boundaries, the authors propose a new method called the "weighted line site" method, in which a prior structural image is employed in a modified updating scheme for the boundary variable used in the iterative Bayesian reconstruction. This modified scheme is based on the joint probability of structural and functional boundaries. As to the structural information provided by CT or MR images, only those which have high joint probability with the corresponding PET data are used; whereas other boundary information that is not supported by the PET image is suppressed. The new method has been validated by computer simulation and phantom studies. The results of these validation studies indicate that this new method offers significant improvements in image quality when compared to other reconstruction algorithms, including the filtered backprojection method and the maximum likelihood approach, as well as the Bayesian method without the use of the prior boundary information.
Cloning, expression, and characterization of the human eosinophil eotaxin receptor. (1996)

Although there is a mounting body of evidence that eosinophils are recruited to sites of allergic inflammation by a number of beta-chemokines, particularly eotaxin and RANTES, the receptor that mediates these actions has not been identified. We have now cloned a G protein-coupled receptor, CC CKR3, from human eosinophils which, when stably expressed in AML14.3D10 cells bound eotaxin, MCP-3 and RANTES with Kds of 0.1, 2.7 and 3.1 nM, respectively. CC CKR3 also bound MCP-1 with lower affinity, but did not bind MIP-1 alpha or MIP-1 beta. Eotaxin, RANTES, and to a lessor extent MCP-3, but not the other chemokines, activated CC CKR3 as determined by their ability to stimulate a Ca(2+) -flux. Competition binding studies on primary eosinophils gave binding affinities for the different chemokines which were indistinguishable from those measured with CC CKR3. Since CC CKR3 is prominently expressed in eosinophils we conclude that CC CKR3 is the eosinophil eotaxin receptor. Eosinophils also express a much lower level of a second chemokine receptor, CC CKR1, which appears to be responsible for the effects of MIP-1 alpha.