gebhart/patrepeval huggingface dataset:
Characterization of the insulin receptor kinase purified from human placental membranes. (1983)

The insulin receptor purified from human placenta by sequential affinity chromatography on wheat germ agglutinin- and insulin-Sepharose to near homogeneity retained tyrosine-specific protein kinase activity. This purified insulin receptor kinase specifically catalyzed the incorporation of 32P from [gamma-32P]ATP into not only the beta-subunit of the insulin receptor but also histone H2B, a synthetic peptide which is sequentially similar to the site of tyrosine phosphorylation in pp60src (a gene product of the Rous sarcoma virus) and antibodies to pp60src present in the sera obtained from three rabbits bearing tumors induced by the Rous sarcoma virus. In each case, phosphorylation occurred exclusively on tyrosine residues. Insulin stimulated phosphorylation of these substrates 3- to 5-fold. Kinetic analysis using the synthetic peptide indicated that insulin acted by increasing the Vmax of peptide phosphorylation from about 3.1 to 9.5 nmol X mg-1 of protein X min-1, whereas the value of the Km for the peptide, about 1.5 mM, was not significantly changed. This kinase acted weakly on casein, alpha-S-casein, actin, and a tyrosine-containing peptide analogue of a serine-containing peptide used commonly as a substrate for the cyclic AMP-dependent protein kinases. These data show that the insulin receptor kinase displays specificity toward exogenous substrates similar to the substrate specificity observed for pp60src and the protein kinase activity associated with the receptor for epidermal growth factor. The data suggest that the catalytic sites of these three tyrosine kinases are similar and that insulin activates its receptor kinase by increasing the Vmax.
High-volume data hiding in images: Introducing perceptual criteria into quantization based embedding (2002)

Information-theoretic analyses for data hiding prescribe embedding the hidden data in the choice of quantizer for the host data. In this paper, we consider a suboptimal implementation of this prescription, with a view to hiding high volumes of data in images with low perceptual degradation. Our two main findings are as follows: (a) In order to limit perceptual distortion while hiding large amounts of data, the hiding scheme must use perceptual criteria in addition to information-theoretic guidelines. (b) By focusing on “benign” JPEG compression attacks, we are able to attain very high volumes of embedded data, comparable to information-theoretic capacity estimates for the more malicious Additive White Gaussian Noise (AWGN) attack channel, using relatively simple embedding techniques.
Early myotome specification regulates PDGFA expression and axial skeleton development (2000)

Reciprocal defects in signaling between the myotome and the sclerotome compartments of the somites in PDGFRalpha and Myf5 mutant embryos lead to alterations in the formation of the vertebrae and the ribs. To investigate the significance of these observations, we have examined the role of PDGF signaling in the developing somite. PDGFA ligand expression was not detected in the myotome of Myf5 null mutant embryos and PDGFA promoter activity was regulated by Myf5 in vitro. PDGFA stimulated chondrogenesis in somite micromass cultures as well as in embryos when PDGFA was knocked into the Myf5 locus, resulting in increased vertebral and rib development. PDGFA expression in the myotome was fully restored in embryos in which MyoD has been introduced at the Myf5 locus but to a lesser extent in similar myogenin knock-in embryos. These results underscore the importance of growth factor signaling within the developing somite and suggest an important role for myogenic determination factors in orchestrating normal development of the axial skeleton.
Single-electron tunneling effects in granular metal films (1994)

Cryogenic scanning tunneling microscopy is used to study local electrical-transport properties of thin granular Au/${\mathrm{Al}}_{2}$${\mathrm{O}}_{3}$ films in the vicinity of the percolation threshold. The current-voltage characteristics are found to vary dramatically from one tip position to another over distances of the order of a few nanometers. These characteristics often exhibit interesting Coulomb-staircase structures having unusual variations in step widths and heights due to complex tunneling paths. A triple-barrier tunnel-junction model accounts quantitatively for the experimental results.
Myelin-Associated Glycoprotein as a Functional Ligand for the Nogo-66 Receptor (2002)

Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and myelin-associated glycoprotein (MAG). The receptor for Nogo (NgR) has been identified as an axonal glycosyl-phosphatidyl-inositol (GPI)-anchored protein, whereas the MAG receptor has remained elusive. Here, we show that MAG binds directly, with high affinity, to NgR. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons are rendered MAG-sensitive by expression of NgR. MAG and Nogo-66 activate NgR independently and serve as redundant NgR ligands that may limit axonal regeneration after CNS injury.
Samples Used in Strategic Management Research. (1987)

A content analysis of the samples used in 136 empirical studies published in three mainstream strategic management journals is conducted. The samples are categorized based on the sampling techniques employed by researchers. Results suggest that information on sampling techniques provided in articles is incomplete, and that researchers are biased toward sampling large organizations and certain industries.
Cerebral Protection, Brain Repair, Plasticity and Cell Therapy in Ischemic Stroke (2009)

Among available treatments in the acute ischemic stroke, only intravenous thrombolysis has been demonstrated to be efficacious. Although the majority of pharmacological neuroprotectants have been efficacious in experimental studies, they have failed in clinical trials. Hence, we need to consider integrated cerebral protection which includes the concept of cerebral repair by supporting cerebral plasticity. We provide a nonsystematic review of the studies published on cerebral protection and repair treatments of cerebral ischemia considering the possibilities of stimulating brain plasticity by trophic factors and cell therapy. The majority of the neuroprotective drugs have failed in clinical trials. Citicoline shows a benefit in meta-analysis and it is currently being explored in a new trial (ICTUS). Neuroprotective drugs combined with reperfusion offer favorable results in experimental animals, but data from clinical studies are not enough. Repair therapies using cerebral plasticity stimulation (trophic factors) and cell therapy have shown certain efficacy in experimental and clinical studies and they are a developing route with clinical therapeutic perspectives.
Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen (2001)

In human cells APE1 is the major AP endonuclease and it has been reported to have no functional mitochondrial targeting sequence (MTS). We found that APE2 protein possesses a putative MTS. When its N-terminal 15 amino acid residues were fused to the N-terminus of green fluorescent protein and transiently expressed in HeLa cells the fusion protein was localized in the mitochondria. By electron microscopic immunocytochemistry we detected authentic APE2 protein in mitochondria from HeLa cells. Western blotting of the subcellular fraction of HeLa cells revealed most of the APE2 protein to be localized in the nuclei. We found a putative proliferating cell nuclear antigen (PCNA)-binding motif in the C-terminal region of APE2 and showed this motif to be functional by immunoprecipitation and in vitro pull-down binding assays. Laser scanning immunofluorescence microscopy of HeLa cells demonstrated both APE2 and PCNA to form foci in the nucleus and also to be co-localized in some of the foci. The incubation of HeLa cells in HAT medium containing deoxyuridine significantly increased the number of foci in which both molecules were co-localized. Our results suggest that APE2 participates in both nuclear and mitochondrial BER and also that nuclear APE2 functions in the PCNA-dependent BER pathway.
Client-side dynamic metadata in web 2.0 (2007)

Web 2.0 brings with it new opportunities for deploying highly interactive webpages. A challenge for Web 2.0 developers is capturing information to document the user experience. Client-side dynamic metadata provides one solution to capturing and logging information on the client-side, while lowering the bandwidth requirements for communication with the server. This paper discusses the architecture of a proof-of-concept system, the Dynamic Metadata Prototype. The architecture is described as an AJAX Design Pattern.
Mechanistic studies show that (−)‐FTC‐TP is a better inhibitor of HIV‐1 reverse transcriptase than 3TC‐TP (1999)

Of all of the nucleoside inhibitors approved by the FDA for treatment of AIDS, (-)-beta-2',3'-dideoxy-3'-thiacytidine (3TC, lamivudine) is the only one with the unnatural (-)-beta-L configuration. The fluorinated derivative (-)-beta-2', 3'-dideoxy-5-fluoro-3'-thiacytidine [(-)-FTC] and its triphosphate form have also been reported to have excellent antiretroviral activity against HIV-1 reverse transcriptase (RT). Preliminary results of clinical trials suggest that (-)-FTC is 6- to 10-fold more potent than 3TC. However, the molecular mechanism for the observed enhanced clinical potency of (-)-FTC to inhibit viral replication is not understood. The present mechanistic studies used a transient kinetic approach and were designed to compare the incorporation of 3TC-TP and (-)-FTC-TP into DNA by HIV-1 RT and illuminate key features that may play a role in the differential potency. Here we show that (-)-FTC-TP is incorporated 10-fold more efficiently than 3TC-TP during HIV-1 RT-catalyzed RNA-dependent DNA synthesis. The enhanced incorporation efficiency of (-)-FTC-TP may be a key mechanistic feature that, in part, is responsible for the enhanced potency of (-)-FTC observed in ongoing clinical trials.
Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes (2002)

An important consideration in the design of oligonucleotide probes for homogeneous hybridization assays is the efficiency of energy transfer between the fluorophore and quencher used to label the probes. We have determined the efficiency of energy transfer for a large number of combinations of commonly used fluorophores and quenchers. We have also measured the quenching effect of nucleotides on the fluorescence of each fluorophore. Quenching efficiencies were measured for both the resonance energy transfer and the static modes of quenching. We found that, in addition to their photochemical characteristics, the tendency of the fluorophore and the quencher to bind to each other has a strong influence on quenching efficiency. The availability of these measurements should facilitate the design of oligonucleotide probes that contain interactive fluorophores and quenchers, including competitive hybridization probes, adjacent probes, TaqMan probes and molecular beacons.
Separation of close-boiling hydrocarbons with silicalite zeolite membranes (1996)

The permeation behaviour of n-heptane, isooctane and n-hexane vapours through continuous silicalite zeolite membranes on porous alumina supports has been investigated between 359 and 523 K. Pure isooctane, n-heptane and n-hexane have similar permeances. In the presence of n-heptane and n-hexane, in both binary and ternary mixtures, however, the isooctane permeance decreases by a factor of up to ca. 50, whereas the n-heptane and n-hexane permeances are almost the same as the pure component permeances. Thus, separation selectivities for n-heptane–isooctane as high as 138 were obtained at 373 K in ternary mixtures with n-hexane, but such separations cannot be predicted from the pure gas permeances. Selectivity is a strong function of temperature, and lower selectivities were obtained above and below 373 K for the ternary mixture. The highest selectivity of 57 for a binary n-heptane–isooctane mixture was obtained at 359 K, and selectivity decreased with increasing temperature. The permeances of all compounds in the mixtures increase with temperature.
Recombinant Human Hexamer-Dominant IgM Monoclonal Antibody to Ganglioside GM3 for Treatment of Melanoma (2007)

A hexamer-dominant human IgM against GM3 may provide a more potent treatment option for patients with GM3-positive melanoma.
Pulsed Electric Fields: Processing System, Microbial and Enzyme Inhibition, and Shelf Life Extension of Foods (2007)

Pulsed electric field (PEF) nonthermal food processing has been of growing interest owing to because of its excellent potential in providing consumers with microbiologically safe and fresh quality foods. Application of high-voltage electric fields at a certain level for a very short time by PEF not only inhibits pathogenic and spoilage microorganisms but also results in the retention of flavor, aroma, nutrients, and color of foods. This paper provides the most current information about PEF food processing. It reviews the systems for PEF processing and its effects on the inhibitions of microorganisms and enzymes and sensory and nutritional properties of foods. Regulatory issues of PEF processing are discussed as well
Akt Inhibitor A-443654 Interferes with Mitotic Progression by Regulating Aurora A Kinase Expression (2008)

Both Akt and Aurora A kinase have been shown to be important targets for intervention for cancer therapy. We report here that Compound A (A-443654), a specific Akt inhibitor, interferes with mitotic progression and bipolar spindle formation. Compound A induces G(2)/M accumulation, defects in centrosome separation, and formation of either monopolar arrays or disorganized spindles. On the basis of gene expression array studies, we identified Aurora A as one of the genes regulated transcriptionally by Akt inhibitors including Compound A. Inhibition of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, either by PI3K inhibitor LY294002 or by Compound A, dramatically inhibits the promoter activity of Aurora A, whereas the mammalian target of rapamycin inhibitor has little effect, suggesting that Akt might be responsible for up-regulating Aurora A for mitotic progression. Further analysis of the Aurora A promoter region indicates that the Ets element but not the Sp1 element is required for Compound A-sensitive transcriptional control of Aurora A. Overexpression of Aurora A in cells treated with Compound A attenuates the mitotic arrest and the defects in bipolar spindle formation induced by Akt inhibition. Our studies suggest that that Akt may promote mitotic progression through the transcriptional regulation of Aurora A.
Tactile sensor with 3-axis force and vibration sensing functions and its application to detect rotational slip (2002)

This paper concerns a new tactile sensor with both 3-axis force sensing and slip sensing functions. The first three axis force sensing is achieved with tactile heads, each of which is supported by three pressure sensing elements. The pressure sensing function is achieved with arrays of pressure transducers that measure a change in the contact resistance between a specially treated polyimide film and a resistive substrate. The slip sensing function is achieved through the use of a stress-rate sensor that responds to small-scale variations in a surface profile with roughness. Static and dynamic analyses of the new tactile sensor were made and compared with experimental results. Further experiments were conducted with an algorithm to detect rotational slip.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Predictive Value of Access Blood Flow in Detecting Access Thrombosis (1998)

The aim of this study was to evaluate whether repeated measurement of access blood flow (Qac) using the ultrasound dilution technique could predict access failure in patients on hemodialysis. One hundred thirty-one patients were evaluated at intervals of 8 weeks for a period of 6 months. The incidence of thrombosis was determined within each study period. During the 6 month follow-up, 36 thrombotic events occurred in 27 of 68 polytetrafluoroethylene (PTFE) grafts, and only six thrombotic events in 5 of 63 arteriovenous (AV) fistulas. The relative risk for access thrombosis for patients with PTFE grafts was 5.6 times greater than for patients with AV fistulas. Qac was significantly lower in thrombotic compared with patent PTFE grafts (958 +/- 506 ml/min vs 1141 +/- 482 ml/min, p < 0.05). A significant relationship was found between the incidence of subsequent PTFE graft thrombotic events and Qac (p < 0.001). Compared with accesses with high blood flow (1100-1400 ml/min), the risk for subsequent thrombosis tripled in grafts with a Qac of less than 500 ml/min. This relationship was not seen with AV fistulas. In patent PTFE grafts, Qac remained unchanged within each 2 month interval, whereas it decreased in thrombotic PTFE grafts. Thus, repeated measurements of Qac have the potential to predict future access failure in PTFE grafts; however, an increased measuring frequency might improve the predictive value of graft failure with high Qac.
Model-based 3D Hand Tracking with on-line Shape Adaptation (2015)

One of the shortcomings of the existing model-based 3D hand tracking methods is the fact that they consider a fixed hand model, i.e. one with fixed shape parameters. In this work we propose an online model-based method that tackles jointly the hand pose tracking and the hand shape estimation problems. The hand pose is estimated using a hierarchical particle filter. The hand shape is estimated by fitting the shape model parameters over the observations in a frame history. The candidate shapes required by the fitting framework are obtained by optimizing the shape parameters independently in each frame. Extensive experiments demonstrate that the proposed method tracks the pose of the hand and estimates its shape parameters accurately, even under heavy noise and inaccurate shape initialization.
Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts. (1992)

Cytomegalovirus (CMV) infection has been associated with the development of accelerated arteriosclerosis in heart transplant recipients. This association prompted the authors to examine, using in situ hybridization techniques, the coronary arteries of 19 patients who had received heart transplants for the presence of CMV nucleic acids. Two probes were employed on formalin-fixed paraffin-embedded tissues. The first was an S35-labeled nick translated probe derived from CMV DNA. CMV nucleic acids were identified in the arterial intima of 5 (45%) of 11 transplanted hearts examined with this probe. Hybridization to cells morphologically consistent with endothelial cells, lymphocytes, and smooth muscle cells was identified. Hybridization was not detected in any of the hearts when a control probe, a vector without CMV DNA, was employed. The second probe that was used was a tritium-labeled strand-specific ribo-probe derived from the immediate early (IE1) gene of CMV. Using this second probe, hybridization was detected in the coronary arteries of three of five hearts that were positive with the DNA probe, in one of the hearts that was negative with the DNA probe, and in three (38%) of eight additional transplanted hearts. The pattern of hybridization was the same as that seen with the DNA probe, and no hybridization was detected when a control sense probe for the IE1 gene of CMV was employed. These results suggest that CMV nucleic acids are present in the coronary arteries of heart transplant recipients.
Differential Immunogenicity of Various Heterologous Prime-Boost Vaccine Regimens Using DNA and Viral Vectors in Healthy Volunteers (2005)

Heterologous prime-boost vaccination has been shown to be an efficient way of inducing T cell responses in animals and in humans. We have used three vaccine vectors, naked DNA, modified vaccinia virus Ankara (MVA), and attenuated fowlpox strain, FP9, for prime-boost vaccination approaches against Plasmodium falciparum malaria in humans. In this study, we characterize, using two types of ELISPOT assays and FACS analysis, cell-mediated immune responses induced by different prime-boost combinations where all vectors encode a multiepitope string fused to the pre-erythrocytic Ag thrombospondin-related adhesion protein. We show that these different vectors need to be used in a specific order for an optimal ex vivo IFN-gamma response. From the different combinations, DNA priming followed by MVA boosting and FP9 priming followed by MVA boosting were most immunogenic and in both cases the IFN-gamma response was of broad specificity and cross-reactive against two P. falciparum strains (3D7 and T9/96). Immunization with all three vectors showed no improvement over optimal two vector regimes. Strong ex vivo IFN-gamma responses peaked 1 wk after the booster dose, but cultured ELISPOT assays revealed longer-lasting T cell memory responses for at least 6 mo. In the DNA-primed vaccinees the IFN-gamma response was mainly due to CD4(+) T cells, whereas in the FP9-primed vaccinees it was mainly due to CD4-dependent CD8(+) T cells. This difference may be of importance for the protective efficacy of these vaccination approaches against various diseases.
Nonlinear filtering of multiplied and convolved signals (1968)

An approach to some nonlinear filtering problems through a generalized notion of superposition has proven useful In this paper this approach is investigated for the nonlinear filtering of signals which can be expressed as products or as convolutions of components. The applications of this approach in audio dynamic range compression and expansion, image enhancement with applications to bandwidth reduction, echo removal, and speech waveform processing are presented.
RNA polymerase-dependent mechanism for the stepwise T7 phage DNA transport from the virion into E. coli (1982)

The influence of rifampicin, streptolydigin, tetracycline and chloramphenicol on phage DNA transport from the T7 virion into the E. coli cell was studied. It has been found that the DNA transport proceeds in at least three stages. During the initial stage the phage injects into the host cell the left approximately 10 per cent of its DNA molecule. The entrance of the next 50 per cent of 17 DNA molecule is blocked by inhibitors which block transcription but not translation. Moreover, the entrance time of this part of the T7 DNA increases in the case of the T7 mutant D111 (which contains a deletion of the A2 and A3 promoters) and decreases in the case of the D53 mutant (which contains a deletion in the region of the early gene transcription terminator). It would appear, that the second stage of the phage DNA transport is tightly coupled with its transcription and that a mechanical function is carried out by RNA polymerase. The translation inhibitors completely block the entrance of the remaining 40 per cent of the 17 DNA molecule (class III genes) into the host cell. It would appear that some class I and (or) II gene product(s) are obligatory components of the final stage of 17 DNA transport. Some probable consequences of this virus DNA transport model as well as its agreement with the functional structure of T7 chromosome and with T7 development are discussed.