gebhart/patrepeval huggingface dataset:
Molecular Cloning of cDNA Encoding Rat Very Long-chain Acyl-CoA Synthetase (1996)

The cDNA encoding rat very long-chain acyl-CoA synthetase (VLACS) was cloned, using degenerative primers synthesized according to the partial amino acid sequences of the peptide fragments of the purified rat liver enzyme. The longest cDNA insert was 2972 base pairs with a 1860-base pair open reading frame encoding 620 amino acids. The calculated molecular mass of 70,692 daltons was consistent with size of the purified enzyme. In Northern blot analysis, a single band was detected at the position of about 3 kilobases, corresponding to the size of the cloned cDNA. cDNA-directed expression in Escherichia coli resulted in accumulation of expressed protein, as an inclusion body. An antibody was raised using this expressed protein to characterize the cDNA and the enzyme. The subcellular localization of VLACS in peroxisomes and microsomes was demonstrated in Western blot analysis. The specific activity and the substrate specificity of the cDNA expressed enzyme in COS-1 cells were consistent with those of the purified rat enzyme. The predicted amino acid sequence of VLACS had a high sequence similarity to fatty acid transport protein (Schaffer, J. E., and Lodish, H. F. (1994) Cell 79, 427-436), and was considered to have domains for adenylation and thioester formation. The entire structure of VLACS was dissimilar to that of long-chain acyl-CoA synthetase (Suzuki, H., Kawarabayashi, Y., Kondo, Y., Abe, T., Nishikawa, K., Kimura, S., Hashimoto, T., and Yamamoto, T. (1990) J. Biol. Chem. 265, 8681-8685), except for the domains.
A Food-Grade Cloning System for Industrial Strains of Lactococcus lactis (2000)

We have previously reported the construction of a food-grade cloning vector for Lactococcus using the ochre suppressor, supB, as the selective marker. This vector, pFG1, causes only a slight growth inhibition in the laboratory strain MG1363 but is unstable in the industrial strains tested. As supB suppresses both amber and ochre stop codons, which are present in 82% of all known lactococcal genes, this undesirable finding may result from the accumulation of elongated mistranslated polypeptides. Here, we report the development of a new food-grade cloning vector, pFG200, which is suitable for overexpressing a variety of genes in industrial strains of Lactococcus lactis. The vector uses an amber suppressor, supD, as selectable marker and consists entirely of Lactococcus DNA, with the exception of a small polylinker region. Using suppressible pyrimidine auxotrophs, selection and maintenance are efficient in any pyrimidine-free medium including milk. Importantly, the presence of this vector in a variety of industrial strains has no significant effect on the growth rate or the rate of acidification in milk, making this an ideal system for food-grade modification of industrially relevant L. lactis strains. The usefulness of this system is demonstrated by overexpressing the pepN gene in a number of industrial backgrounds.
A Putative New Retrovirus Associated with Multiple Sclerosis and the Possible Involvement of Epstein‐Barr Virus in This Diseasea (1994)

Since tropical spastic paraparesis in 1985 was found to be associated with HTLV-I infection, it has been suggested that a retrovirus might be involved in multiple sclerosis (MS). Our group has studied long-term cultures of cerebrospinal fluid cells and peripheral blood mononuclear cells from MS patients and controls with the purpose of elucidating the possible involvement of a retrovirus in MS. For an extended period electron microscopical analysis (EM) of T-cell lines, derived from MS patients and controls and cultured for 4 weeks was performed. In two cultures obtained 8 months apart from a patient with progressive MS, retrovirus-like particles were observed in 1-2% of the cells examined. Recently a B-lymphoblastoid cell line (LCL) producing retrovirus-like particles and EBV was established from a 30-year-old male patient with a chronic progressive myelopathy, clinically resembling multiple sclerosis. Similar cell lines have now been established from two MS patients. The retrovirus-like particles produced by the LCL have been purified by gradient ultracentrifugation. In the purified material reverse transcriptase assays are clearly positive in the gradients where EM shows retrovirus-like particles. Antigen characterization, nucleic acid sequence analysis and antibody studies are now being performed. The retrovirus found is definitively different from other known human retroviruses. It has previously been found that 100% of patients with MS have antibodies against EBV, in contrast to controls where only 86-95% have antibodies against this virus. Previous epidemiological studies have pointed toward a post-pubertal primary EBV infection as an important event in the induction of MS disease. These studies have now been substantiated by our group. Though it is still unknown whether EBV infection is a prerequisite for development of MS or whether the 100% EBV seropositivity is a consequence of the MS disease, we have put forward the hypothesis that the etiological agent for development of MS and MS-like diseases is a new hitherto uncharacterized retrovirus, whereas development of neurologic disease is related to or even dependent on a delayed infection with a virus from the herpes group, most likely EBV. This dual infection hypothesis has been analyzed and was found to be in accordance with the most consistent epidemiological characteristics of MS. We have previously, also from epidemiological data, negated retroviruses, behaving as the known human retroviruses, as an independent cause of MS.
Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines (2004)

Chromosomal position effects are the cause of variegation of transgene expression relating to the eukaryotic genome environment. These effects modify heterologous transgene expression, presumably due to dominant epigenetic marks that cause variegated or gradual silencing of transgene expression. As a solution, chromosomal insulators have arisen as a way to protect transgene expression against repressive marks (position effects), allowing homogeneous and consistent expression. Sustainable expression enables us to gain insight into in vivo protein function and protein stability, or to study position effects. Here, we show that the chicken cHS4 beta-globin insulator is able to protect transgene expression in cell lines, demonstrating its potential as a tool to improve the heterologous expression of any desired transgene. In addition, we show that green fluorescent protein (GFP) can be used as a reporter protein in the study of chromosomal position effects.
Flow cytometric sperm sorting: Effects of varying laser power on embryo development in swine* (2001)

This study was conducted to determine fertilization rate and embryo development using the Beltsville Sperm Sexing Technology with two different laser power outputs, 25 and 125 milliwatts (mW). Freshly ejaculated boar semen was diluted; one aliquot was not stained or sorted (nonsort) and a second aliquot was stained with Hoechst 33342 and sorted as a complete population, not separated into X and Y populations (all-sort). Ovulation controlled gilts were surgically inseminated with 2 x 10(5) spermatozoa (44-46 hr after human chorionic gonadotropin (hCG)) into the isthmus of each oviduct, one oviduct receiving nonsort and the other all-sort at 25 or 125 mW. A total of 426 embryos were flushed from oviducts at slaughter 43 hr after laparotomy and prepared for determination of fertilization and cleavage rates using confocal laser microscopy for analysis of actin cytoskeleton and chromatin configuration. The percentage of fertilized eggs and embryos was less for the 25 mW all-sort compared to nonsort or the 125 mW all-sort (77.9 vs. 96.3 and 96.2%, P < 0.05). The percentage of fragmented embryos was greater for the 25 mW all-sort than the nonsort (15.2 vs. 4.5%, P < 0.05), but did not differ significantly from 125 mW all-sort mean (7.2%). The percentage of normal embryos (80.4% overall) did not differ (P > 0.05) among treatments. However, the rate of embryo development was slower (P < 0.05) after insemination with the 25 mW all-sort spermatozoa compared to nonsort spermatozoa. Embryos in the 3-4 and 5-9 cell stages for the 25-mW all-sort and nonsort were 78 and 20% vs. 49 and 50%, respectively. The embryo percentages for the 125 mW (3-4 and 5-9 cell stages, 59 and 35%) did not differ significantly (P > 0.05) from the nonsort or 25 mW all-sort. We conclude that the use of 125 mW laser power for sorting boar spermatozoa is advantageous to maintain high resolution separation and has no detrimental effect on embryo development compared to 25 mW.
Circulating and tumor‐infiltrating myeloid cell subsets in patients with bladder cancer (2011)

Both cancer-related inflammation and tumor-induced immune suppression are associated with expansion of myeloid cell subsets including myeloid-derived suppressor cells. However, little known regarding characteristics of myeloid cells in patients with bladder cancer. In this study, we analyzed myeloid cells from peripheral blood (PBMC) and tumor tissue that were collected from patients with superficial noninvasive and invasive urothelial carcinomas. Our results demonstrate that PBMC from bladder cancer patients contain two major CD11b myeloid cell subsets: granulocyte-type CD15(high) CD33(low) cells and monocyte-type CD15(low) CD33(high) cells. The number of circulating granulocytic but not monocytic myeloid cells in cancer patients was markedly increased when compared to healthy individuals. Both myeloid cell subsets from cancer patients were highly activated and produced substantial amounts of proinflammatory chemokines/cytokines including CCL2, CCL3, CCL4, G-CSF, IL-8 and IL-6. Granulocytic myeloid cells were able to inhibit in vitro T cell proliferation through induction of CD4(+) Foxp3(+) T regulatory cells. Analysis of bladder cancer tissues revealed that tumors were infiltrated with monocyte-macrophage CD11b(+) HLA-DR(+) and granulocytic CD11b(+) CD15(+) HLA-DR(-) myeloid cells. Collectively, this study identifies myeloid cell subsets in patients with bladder cancer. We demonstrate that these highly activated inflammatory myeloid cells represent a source of multiple chemokines/cytokines and may contribute to inflammation and immune dysfunction in bladder cancer.
Physical origin of photonic energy gaps in the propagation of surface plasmons on gratings (1996)

We present an analytic model to describe the existence of photonic energy gaps in the propagation of surface plasmon polaritons on corrugated surfaces. We concentrate on elucidating the physical origin of the band gap, and accordingly we place strong emphasis on the physical reasoning and assumptions that we use. Our model is designed to give direct access to expressions for the electromagnetic field and surface charge distributions associated with modes at the band edges, thus allowing their physical character to be easily appreciated. Having established why a band gap occurs we then find expressions for the central position and width of the gap. We compare the results of our model for the gap width with those already in the literature, and find excellent agreement. Our results for the central position of the gap, notably the prediction that it should fall as the corrugation amplitude rises, contradicts one prediction made in the literature. We also reexamine the comparisons made in the literature between experiment and theory for the gap width, and find them inadequate because the theories have been compared to inappropriate experimental data. Consequently we present our own recent experimental data, enabling us to validate our theoretical results, in particular confirming our prediction that the central position of the gap falls as the corrugation amplitude is increased. The limitations of our model are discussed, as well as possible extensions and areas for future research.
A 28 GHz HBT Vector Modulator and its Application to an LMCS Feedforward Power Amplifier (1998)

A vector modulator employing four amplitude modulated orthogonal channels is presented. Operating at 28 GHz, this device consists of a four-way 6 dB power divider, four GaAs HBT MMIC variable gain amplifiers, and a 4-to-1 power combiner All of the passive functions were realised on alumina ceramic while the amplifier chips were fabricated at Nortel Technologies using a 3.5 μm × 6 μm GaInP-GaAs HBT process. By testing a vector modulator prototype, we were able to evaluate the architecture and be the first to assess its suitability as a gain/phase control element for a Ka-band MCS adaptive feedforward lineariser.
Acoustic echo control. An application of very-high-order adaptive filters (1999)

We have discussed the application of high-order adaptive filters to the problem of acoustical echo cancellation with particular application to hands free telephone systems. We described a means to achieve robust performance. We further presented methods for reducing computational complexity that allow implementation in low-cost, fixed-point digital signal processors. Progress in technology will allow the use of more sophisticated algorithms at lower cost in the near future.
The environmental state under pressure: An introduction (2005)

Environmental quality has traditionally been perceived as a common or public good which cannot readily be provided via the market, and thus the state has been assumed to be the principal actor in environmental protection (albeit with great variations in the processes and efficacy of state environmental management institutions across nation-states). It is worthwhile noting, however, that the presumption of state responsibility for ensuring environmental quality is a relatively recent phenomenon. Particularly during the early years of nature protection at the turn of the twentieth century it was generally not the state, but rather civil society groups, that took the initiative in safeguarding natural areas and protecting the habitats of endangered species (Hays, 1987). The growing middle class was the key driving force behind the notion of preserving national parks and endangered species. Ultimately, though, states played growing roles in codifying these notions of nature protection in the form of government regulations and establishment of state-protected natural areas (Andrews, 1999). In the industrialized countries of North America, Europe, and Oceania the institutionalization of environmental tasks in state policies and politics and
Antiproliferative Effects on Tumour Cells and Promotion of Keratinocyte Wound Healing by Different Lichen Compounds (2009)

Five compounds representative of major structural classes of lichen polyketides, VIZ. (+)-usnic (1), salazinic (2), vulpinic (3), gyrophoric (4), and evernic acids (5), were investigated for their ability to affect cell proliferation or wound healing, two functional targets of relevance for research on cancer or tissue regeneration. The experiments were carried out on MM98 malignant mesothelioma cells, A431 vulvar carcinoma cells, and HaCaT keratinocytes. The NRU and CV cytotoxicity assays showed high toxicity for (+)-usnic acid, intermediate toxicity for vulpinic acid, and low toxicity for salazinic, gyrophoric and evernic acids. Scratch wounding experiments on HaCaT monolayers, in the presence of subtoxic doses of lichen compounds, showed strong wound closure effects by (+)-usnic and gyrophoric acid, an intermediate effect by vulpinic and salazinic acids, and no effect by evernic acid. A combination of (+)-usnic and gyrophoric acids gave a further increase in the wound closure rates. The results of a cell migration test correlated with the wound healing data. In conclusion, (+)-usnic acid might be a particularly interesting compound for the prevention of hyperproliferation syndromes, while (+)-usnic and gyrophoric acids qualify as interesting leads in the promotion of tissue regeneration.
C. elegans as a model organism to investigate molecular pathways involved with Parkinson's disease (2010)

Parkinson's disease (PD) is an age-related movement disorder resulting, in part, from selective loss of dopaminergic neurons. Both invertebrate and mammalian models have been developed to study the cellular mechanisms altered during disease progression; nevertheless there are limitations within each model. Mammalian models remain invaluable in studying PD, but are expensive and time consuming. Here, we review genetic and environmental factors associated with PD, and describe how the nematode roundworm, Caenorhabditis elegans, has been used as a model organism for studying various aspects of this neurodegenerative disease. Both genetic and chemical screens have been conducted in C. elegans to identify molecular pathways, proteins, and small molecules that can impact PD pathology. Lastly, we highlight future areas of investigation, in the context of emerging fields in biology, where the nematode can be exploited to provide mechanistic insights and potential strategies to accelerate the path toward possible therapeutic intervention for PD.
‘Living’ Controlled in Situ Gelling Systems: Thiol−Disulfide Exchange Method toward Tailor-Made Biodegradable Hydrogels (2010)

A 'living' controlled hydrogel formation method was first reported to create loose and compact in situ biodegradable hydrogels. The method executed under mild reaction conditions can conveniently tailor the hydrogel properties, and it has the potential to develop into a powerful tool for the design, synthesis, and self-assembly of novel tailor-made biomaterials and drug delivery systems.
Rapid in vivo proton shimming (1991)

A rapid and completely automated method of adjusting the magnetic field (B0) homogeneity for in vivo proton spectroscopy and imaging is described. B0 inhomogeneity maps are generated by a gradient-recalled echo pulse sequence in which the frequency dispersion is chosen to eliminate the effects of the fat/water chemical shift. Low-order shim values are derived by magnitude-weighted least-squares fits to the B0 maps and automatically applied as DC offsets to the X, Y, and Z gradient amplifiers. Imaging with chemical shift selective saturation is used as a measure of the efficacy of the technique. Results indicate that AUTOSHIM improves the overall homogeneity: however, local high-order field distortions which cannot be corrected by linear gradients are generated by certain air/tissue and bone/tissue morphology. In such cases a "Zoom SHIM" may be applied over a limited region of interest for local homogeneity improvement at the expense of other regions. It is suggested that such scans are a necessity for recording the homogeneity during clinical MR spectroscopy.
Replication and expression in mammalian cells of tramfected DNA; description of an improved erythrocyte ghost fusion technique (1983)

After modification of an erythrocyte - ghost fusion technique, DNA can be transferred in large amounts to mammalian cells. The overall recovery permits analysis of transferred DNA immediately after fusion by e.g. Southern - blot hybridization and electron microscopy. In general more than 20% of the cells contain the transfected DNA in their nuclei within 4 hours post fusion. Expression of Polyoma T antigens is delayed, as compared to a normal virus infection, and is detected at about 60 hours after transfer of DNA. During the first days an increase in amount of transferred DNA could be detected which might be due to limited replication. To analyze the replication of hybrid DNA molecules consisting of Polyoma and prokaryotic plasmid DNA sequences, fusion was followed by a normal Polyoma virus infection. The resulting induction of cells to enter the S phase made possible replication analysis of transfected hybrid DNA. Replicating molecules of both the theta form and rolling - circle type were observed.
Early alterations of red blood cell rheology in critically ill patients* (2009)

Early alterations of red blood cell rheology are common in intensive care unit patients, especially in those with sepsis. Comorbidities (other than hematologic diseases) do not significantly influence these abnormalities. These alterations could contribute to the microcirculatory alterations observed in critically ill patients.
Synthesis of alkyl-substituted .alpha.,.beta.-unsaturated .gamma.-lactones as potential antitumor agents (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of alkyl-substituted .alpha.,.beta.-unsaturated .gamma.-lactones as potential antitumor agentsGary A. Howie, Peter E. Manni, and John M. CassadyCite this: J. Med. Chem. 1974, 17, 8, 840–843Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://pubs.acs.org/doi/10.1021/jm00254a012https://doi.org/10.1021/jm00254a012research-articleACS PublicationsRequest reuse permissionsArticle Views289Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
CpG DNA Is a Potent Enhancer of Specific Immunity in Mice Immunized with Recombinant Hepatitis B Surface Antigen (1998)

Unmethylated CpG dinucleotides in bacterial DNA or synthetic oligodeoxynucleotides (ODN) cause B cell proliferation and Ig secretion, monocyte cytokine secretion, and activation of NK cell lytic activity and IFN-gamma secretion in vivo and in vitro. The potent immune activation by CpG ODN suggests possible utility for enhancing immune responses to vaccines. Mice immunized with recombinant hepatitis B virus surface Ag and a CpG ODN as an immune enhancer have titers of Abs against HBsAg (anti-HBs) that are five times higher than those of mice immunized with HBsAg and the standard adjuvant, aluminum hydroxide (alum). Ab titers in mice immunized with HBsAg and both CpG ODN plus alum were 35 times higher than the titers in mice immunized with alum alone, indicating a strong synergistic interaction between the CpG ODN and alum. ODN without CpG motifs had little or no immune-enhancing activity at the doses used herein. Alum induces a Th2 humoral response (mostly IgG1) and no CTL. In contrast, CpG ODN gives a strong Thl response with predominantly IgG2a Abs and CTL, even when mixed with alum. In vitro studies to determine possible mechanisms of CpG immune-enhancing effects show that CpG ODN induce expression of costimulatory molecules on Ag-presenting cells and drive B cell isotype switching in the appropriate cytokine milieu. These studies demonstrate that CpG ODN are promising new immune enhancers for vaccination applications.
Estimation of stage‐specific fibrosis progression rates in chronic hepatitis C virus infection (2008)

Our large systematic review provides increased precision in estimating fibrosis progression in chronic HCV infection and supports nonlinear disease progression. Estimates of progression to cirrhosis from studies conducted in clinical settings were lower than previous estimates.