gebhart/patrepeval huggingface dataset:
Molecular and genetic characterization of a radiation-induced structural rearrangement in mouse chromosome 2 causing mutations at the limb deformity and agouti loci. (1990)

Molecular characterization of mutations in the mouse, particularly those involving agent-induced major structural alterations, is proving to be useful for correlating the structure and expression of individual genes with their function in the whole organism. Here we present the characterization of a radiation-induced mutation that simultaneously generated distinct alleles of both the limb deformity (ld) and agouti (a) loci, two developmentally important regions of chromosome 2 normally separated by 20 centimorgans. Cytogenetic analysis revealed that an interstitial segment of chromosome 17 (17B- 17C; or, possibly, 17A2-17B) had been translocated into the distal end of chromosome 2, resulting in a smaller-than-normal chromosome 17 (designated 17del) and a larger form of chromosome 2 (designated 2(17). Additionally, a large interstitial segment of the 2(17) chromosome, immediately adjacent and proximal to the insertion site, did not match bands 2E4-2H1 at corresponding positions on a normal chromosome 2. Molecular analysis detected a DNA rearrangement in which a portion of the ld locus was joined to sequences normally tightly linked to the a locus. This result, along with the genetic and cytogenetic data, suggests that the alleles of ld and a in this radiation-induced mutation, designated ldIn2 and ajIn2, were associated with DNA breaks caused by an inversion of an interstitial segment in the 2(17) chromosome.
Inactivation of the dlt Operon inStaphylococcus aureus Confers Sensitivity to Defensins, Protegrins, and Other Antimicrobial Peptides (1999)

Positively charged antimicrobial peptides with membrane-damaging activity are produced by animals and humans as components of their innate immunity against bacterial infections and also by many bacteria to inhibit competing microorganisms. Staphylococcus aureus and Staphylococcus xylosus, which tolerate high concentrations of several antimicrobial peptides, were mutagenized to identify genes responsible for this insensitivity. Several mutants with increased sensitivity were obtained, which exhibited an altered structure of teichoic acids, major components of the Gram-positive cell wall. The mutant teichoic acids lacked D-alanine, as a result of which the cells carried an increased negative surface charge. The mutant cells bound fewer anionic, but more positively charged proteins. They were sensitive to human defensin HNP1-3, animal-derived protegrins, tachyplesins, and magainin II, and to the bacteria-derived peptides gallidermin and nisin. The mutated genes shared sequence similarity with the dlt genes involved in the transfer of D-alanine into teichoic acids from other Gram-positive bacteria. Wild-type strains bearing additional copies of the dlt operon produced teichoic acids with higher amounts of D-alanine esters, bound cationic proteins less effectively and were less sensitive to antimicrobial peptides. We propose a role of the D-alanine-esterified teichoic acids which occur in many pathogenic bacteria in the protection against human and animal defense systems.
Oval cell proliferation and the origin of small hepatocytes in liver injury induced by D-galactosamine. (1991)

Oval cells may function as facultative liver stem cells and tumor progenitors in liver carcinogenesis. The authors determined whether oval cells proliferate and if small hepatocytes might be generated from epithelial cell progenitors in noncarcinogenic liver injury. The authors found that oval cells similar to those detected in early carcinogenesis proliferate in response to D-galactosamine (GaIN). Oval cells expressed gamma-glutamyl transpeptidase activity, bile duct-type cytokeratins and peanut agglutinin binding. Two unusual types of hepatocytes also appeared after injury: small hepatocytes (less than or equal to 16 microns in diameter) and hepatocytes lining atypical ductlike structures. In situ hybridization studies showed that the fetal form of alphafetoprotein mRNA was expressed by many oval cells, some bile duct cells, and occasional hepatocytes. By following the fate of epithelial cells labeled early after GaIN administration, the authors conclude that duct cells can generate both oval cells and small hepatocytes in response to GaIN.
Front Matter (1996)

Resumen: Este artículo analiza el proceso de implementación del concepto de informalidad en el análisis del mercado laboral colombiano. A la luz de dicho concepto, abordamos la evolución de la informalidad laboral en Colombia, sus principales componentes y características. Utilizando información de la Encuesta de Hogares GEIH-DANE 2010 contrastamos la tasa deinformalidad y su composición, generadas por la definición DANE-PREALC, contra dos definiciones (débil y fuerte), siendo esta última semejante a la sugerida por la CIETDelhi. Nuestros resultados muestran que si bien la tasa de informalidad no parece modificarse en términos de su valor, su composición interna adquiere interesantes patrones, en su mayor parte derivados del hecho de que la definición CIET-Delhi enfatiza el análisis de la informalidad en el puesto de trabajo por encima del de informalidad por tamaño de establecimiento. Estas características plantean importantes cuestionamientos a la forma en que las políticas públicas han enfrentado la informalidad laboral en Colombia.
A new, easily miniaturized steerable endoscope (2005)

This article describes a new steerable endoscope for laparoscopic surgery. The steerable mechanism was inspired by the tentacles of squid and consists only of standard parts such as cables, coil springs, rings, and tubes. Laparoscopic surgery is carried out using an endoscope and long and slender instruments that are inserted through small incisions in the abdominal wall. The endoscope contains a light source and a camera that displays a picture of the abdominal cavity on a monitor in the operation room. The surgeon uses the instruments to carry out the operation while estimating the spatial position of the instruments and the organs from the camera picture. The mechanism can be easily miniaturized to a very small diameter, making it suitable for low-cost mass production of steerable endoscopes, instruments, and catheters. A patent for the mechanism has been applied for, and it is currently being commercialized.
Chemical stability of pharmaceuticals : a handbook for pharmacists (1986)

PRINCIPLES. Stability Calculations. Interpretation of Kinetic Data. Hydrolysis and Other Acyl Transfers. Oxidation and Photolysis. Solid-State Chemical Decompostion. Strategy and Tactics of Stability Testing. STABILITY MONOGRAPHS. Appendixes. Index.
Renal and systemic pH imaging by contrast‐enhanced MRI (2003)

Perturbations of renal and systemic pH accompany diseases of the kidney, such as renal tubular acidosis, and the ability to image tissue pH would be helpful to assess the extent and severity of such conditions. A dual-contrast-agent strategy using two gadolinium agents, the pH-insensitive GdDOTP(5-) and the pH-sensitive GdDOTA-4AmP(5-), has been developed to generate pH maps by MRI. The renal pharmacokinetics of the structurally dissimilar pH-insensitive contrast agents GdDTPA(2-) and GdDOTP(5-) were found to be similar. On that basis, and on the basis of similarity of structure and charge, the renal pharmacokinetics of GdDOTP(5-) and GdDOTA-4AmP(5-) were assumed to be identical. Dynamic T(1)-weighted images of mice were acquired for 1 hr each following boluses of GdDOTP(5-) and GdDOTA-4AmP(5-). The time-varying apparent concentration of GdDOTP(5-) and the time-varying enhancement in longitudinal relaxation rate following GdDOTA-4AmP(5-) were calculated for each pixel and used to compute pH images of the kidneys and surrounding tissues. MRI pH maps of control mice show acidic regions corresponding to the renal papilla, calyx, and ureter. Pretreatment of mice with the carbonic anhydrase inhibitor acetazolamide resulted in systemic metabolic acidosis and accompanying urine alkalinization that was readily detected by this dual-contrast-agent approach.
Improving prediction of secondary structure, local backbone angles and solvent accessible surface area of proteins by iterative deep learning (2015)

Direct prediction of protein structure from sequence is a challenging problem. An effective approach is to break it up into independent sub-problems. These sub-problems such as prediction of protein secondary structure can then be solved independently. In a previous study, we found that an iterative use of predicted secondary structure and backbone torsion angles can further improve secondary structure and torsion angle prediction. In this study, we expand the iterative features to include solvent accessible surface area and backbone angles and dihedrals based on Cα atoms. By using a deep learning neural network in three iterations, we achieved 82% accuracy for secondary structure prediction, 0.76 for the correlation coefficient between predicted and actual solvent accessible surface area, 19° and 30° for mean absolute errors of backbone φ and ψ angles, respectively, and 8° and 32° for mean absolute errors of Cα-based θ and τ angles, respectively, for an independent test dataset of 1199 proteins. The accuracy of the method is slightly lower for 72 CASP 11 targets but much higher than those of model structures from current state-of-the-art techniques. This suggests the potentially beneficial use of these predicted properties for model assessment and ranking.
Market Potential for Interactive Audio-Visual Media (2006)

NM2 (New Media for a New Millennium) develops tools for interactive, personalised and non-linear audio-visual content that will be tested in seven pilot productions. This paper looks at the market potential for these productions from a technological, a business and a users' perspective. It shows that digital broadcast networks and broadband Internet are rapidly growing in Europe, but will have reached less than half of the households in 2007. Broadcasters and television producers have so far invested little in interactive television. New players are platform operators and ISPs, operating as digital content aggregators. Users value possibilities for programme management (EPGs) and simple forms of interactivity (voting, gambling) by using SMS, telephony or Internet. More complex interactive services are not yet very popular. Promising routes are multi-platform and cross media strategies as well as productions for particular target groups that are already actively participating in online communities and games.
Granulocyte-Macrophage Colony-stimulating Factor-activated Signaling Pathways in Human Neutrophils (1999)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) regulates many of the biological activities of human neutrophils. The signaling pathways via which these effects are mediated are not fully understood. We have shown previously that GM-CSF treatment of human neutrophils activates the Janus kinase/signal transducers and activators of transcription (Jak/STAT) pathway and, more specifically, Jak2, STAT3, and STAT5B in neutrophils. GM-CSF also stimulates the activity of the phosphatidylinositol 3-kinase (PI3-kinase) in a tyrosine kinase-dependent manner. Here we report that pretreating the cells with a Jak2 inhibitor (AG-490) abolishes tyrosine phosphorylation of the p85 subunit of PI3-kinase induced by GM-CSF. Furthermore, p85 was found to associate with Jak2, but not with Lyn, in stimulated cells in situ and with its autophosphorylated form in vitro; however, Jak2 did not bind to either of the two Src homology 2 (SH2) domains of the p85 subunit of PI3-kinase. Although STAT5B bound to the carboxyl-terminal SH2 domain of p85, it was absent from the complex containing PI3-kinase and Jak2. These results suggest that stimulation of the activity of PI3-kinase induced by GM-CSF is mediated by Jak2 and that the association between Jak2 and p85 depends on an adaptor protein yet to be identified.
A differential location of phosphoinositide kinases, diacylglycerol kinase, and phospholipase C in the nuclear matrix. (1992)

Several enzymes involved in the phosphoinositide metabolism have been shown to be present in nuclei of rat liver and Friend cells. In this paper we demonstrate that nuclear matrices of mouse NIH 3T3-fibroblasts and rat liver cells, isolated by nuclease treatment and high salt extraction, contain phosphatidylinositol 4-kinase (PdtIns 4-kinase), phosphatidylinositol 4-phosphate 5-kinase (PtdIns(4)P 5-kinase), diacylglycerol kinase, and phospholipase C. By a selective extraction the nucleus can be dissected in the peripheral matrix (lamina-pore complex) and the internal matrix as shown by using marker antibodies. Surprisingly, PtdIns 4-kinase was found exclusively in the peripheral nuclear matrix, whereas PtdIns(4)P 5-kinase was found to be associated to internal matrix structures. Diacylglycerol kinase and phospholipase C activities were also preferentially detected in the internal matrix. These data demonstrate a differential localization of the phosphoinositide kinases in the nucleus and suggest that the phosphoinositide metabolism may play a specific role in the nucleus.
Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic (2009)

In March and early April 2009, a new swine-origin influenza A (H1N1) virus (S-OIV) emerged in Mexico and the United States. During the first few weeks of surveillance, the virus spread worldwide to 30 countries (as of May 11) by human-to-human transmission, causing the World Health Organization to raise its pandemic alert to level 5 of 6. This virus has the potential to develop into the first influenza pandemic of the twenty-first century. Here we use evolutionary analysis to estimate the timescale of the origins and the early development of the S-OIV epidemic. We show that it was derived from several viruses circulating in swine, and that the initial transmission to humans occurred several months before recognition of the outbreak. A phylogenetic estimate of the gaps in genetic surveillance indicates a long period of unsampled ancestry before the S-OIV outbreak, suggesting that the reassortment of swine lineages may have occurred years before emergence in humans, and that the multiple genetic ancestry of S-OIV is not indicative of an artificial origin. Furthermore, the unsampled history of the epidemic means that the nature and location of the genetically closest swine viruses reveal little about the immediate origin of the epidemic, despite the fact that we included a panel of closely related and previously unpublished swine influenza isolates. Our results highlight the need for systematic surveillance of influenza in swine, and provide evidence that the mixing of new genetic elements in swine can result in the emergence of viruses with pandemic potential in humans.
EMERGING TECHNOLOGIES WITH DISRUPTIVE EFFECTS: A REVIEW (2017)

Since the last decade, several technologies have been abruptly influencing our lives. Among the notable major changes in the human society is the way humans interact with each other, which now involves the social media heavily where this is previously unknown to the masses. This type of technology is called Disruptive Innovation, a type of technology which has the potential to alter how human lives, market trends as well as other aspects including transportation and communications. Studies show that in the next few years, there are plenty of technology with the disruptive features. The new technologies, part of the domino effect of previous emerging technologies, include autonomous vehicle, the blockchain as well as Internet-of-Things. Despite the rapid advent, little discussion has been done to discuss and summarizes the emerging technologies in a single work in Malaysia. This brief survey is written as an effort to initiate the discussion, particularly in Malaysia about the disruptive innovation. It gives an introductory idea to the general audience, as well as academician and practitioners about the examples of the innovation, as well as brief discussion about the future studies which need to be commended in relation to the current development. This work is hoped to speed up the implementation of the emerging technologies in Malaysia as well helping and guiding policymakers, practitioners as well as academicians in understanding the phenomenon.
Enhancement of thrombogenesis by plasma fibronectin cross-linked to fibrin and assembled in platelet thrombi (2006)

To learn how plasma fibronectin stabilizes platelet-rich thrombi in injured mesenteric arterioles of mice, we studied the impact of plasma fibronectin on platelet thrombus formation ex vivo in a parallel flow chamber. Thrombi were greater on surfaces coated with fibrin cross-linked to fibronectin by activated factor XIII than on surfaces coated with fibrin lacking cross-linked fibronectin or with fibronectin alone. Platelet thrombi were even greater when plasma fibronectin was perfused with platelets, resulting in deposition of the perfused fibronectin in platelet thrombi. The effect of perfused fibronectin on thrombogenesis was lost if fibronectin deposition was blocked by coperfusion with the N-terminal 70-kDa fragment of fibronectin or a peptide based on the functional upstream domain of protein F1 of Streptococcus pyogenes. Increases in thrombus formation were dependent on a platelet activator such as lysophosphatidic acid, amount of fibronectin cross-linked to fibrin, and concentration of fibronectin in the perfusate. The dependency of fibronectin concentration extended into the range of fibronectin concentrations associated with increased risk of coronary artery disease. At such concentrations, the 2 mechanisms for insolubilization of plasma fibronectin-cross-linking to fibrin and assembly by adherent and aggregating platelets-synergize to result in many-fold enhancement of platelet thrombus formation.
Modelling of isotropic double negative media for microwave applications (2006)

Abstract A composite medium consisting of two sublattices of dielectric spherical particles of high permittivity and different radii embedded in a dielectric matrix of smaller permittivity are considered. It has been shown that such a composite medium reveals properties of an isotropic double negative media (DNG) in a limited frequency range, when resonance oscillations of HIII mode in one kind of particles and EIII mode in another kind of particles are excited simultaneously. The EIII resonance and the HIII resonance give rise to the magnetic dipole momentum and the electric dipole momentum correspondingly. Averaging the magnetic momentum and the electric momentum over the cells belonging to the appropriate spherical particles gives the negative permittivity and permeability. The model of diffraction of a plane electromagnetic wave on a dielectric sphere is presented and compared with the mixing rule based consideration. The results obtained are rather close. Distribution of the electromagnetic wave outside the sphere is calculated. Influence of the dispersion of the sphere size and the dielectric permittivity on the effective parameters of the DNG material is estimated.
Microfabricated devices for genetic diagnostics (1998)

This paper presents a review of microfabricated devices for genetic diagnostics. Genetic diagnostics are powerful technology drivers and excellent candidate applications for miniaturization technologies because the demand for inexpensive genetic information is essentially unlimited, and the cost and time for the diagnostic decreases with sample volume. Genetic information is stored in long DNA molecules in solution. This information is processed and extracted using a series of enzymatic and other chemical reactions well known in molecular biology. Processing of DNA molecules in the microscale hence requires the implementation of microfluidic devices capable of handling, mixing, thermal cycling, separating, and detecting nano- and picoliter liquid samples. This paper discusses some of the fundamental macroscale protocols used for genetic analyses and how these processes scale down to microscopic volumes. The construction and performance of microfluidic devices of DNA amplification, separation, hybridization, and detection are discussed, showing that so far, no fundamental impediments exist for genetic diagnostics based on microelectromechanical systems. Some of the unresolved storage and packaging issues and future challenges for the practical implementation of these devices are also presented.
Mechanisms of Resistance to Crizotinib in Patients with ALK Gene Rearranged Non–Small Cell Lung Cancer (2012)

Crizotinib resistance in ALK(+) NSCLC occurs through somatic kinase domain mutations, ALK gene fusion CNG, and emergence of separate oncogenic drivers.
Mechanism of action of bacterial glycerophospholipid:cholesterol acyltransferase (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism of action of bacterial glycerophospholipid:cholesterol acyltransferaseJ. Thomas BuckleyCite this: Biochemistry 1983, 22, 24, 5490–5493Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published inissue 1 November 1983https://doi.org/10.1021/bi00293a006Request reuse permissionsArticle Views74Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (517 KB) Get e-Alertsclose Get e-Alerts
The shared tumor-specific antigen encoded by mouse geneP1A is a target not only for cytolytic T lymphocytes but also for tumor rejection (1998)

A number of human tumor antigens have been characterized recently using cytolytic T lymphocytes (CTL) as screening tools. Some of them are encoded by MAGE-type genes, which are silent in normal tissues except in male germ cells, but are activated in a variety of tumors. These tumor-specific shared antigens appear to be promising targets for cancer immunotherapy. However, the choice of these antigens as targets has been questioned because of the lack of direct evidence that in vivo responses against such antigens can lead to tumor rejection. The antigen encoded by the mouse gene P1A represents the only available animal model system for MAGE-type tumor antigens. We show here that mice immunized by injection of L1210 leukemia cells expressing P1A and B7-1 (L1210.P1A.B7-1) are efficiently protected against a challenge with a lethal dose of mastocytoma P815 tumor cells, which express P1A. Mice immunized with L1210 cells expressing B7-1 but not P1A were not protected. Furthermore, we observed that P1A-transgenic mice, which are tolerant to P1A, were not protected after immunization with L1210.P1A.B7-1. These results demonstrate that the immune response to P1A is the major component of the tumor rejection response observed in normal mice, and support the use of tumor-specific shared antigens as targets for the immunotherapy of human cancer.