gebhart/patrepeval huggingface dataset:
Attention-Based Recurrent Neural Network Models for Joint Intent Detection and Slot Filling (2016)

Attention-based encoder-decoder neural network models have recently shown promising results in machine translation and speech recognition. In this work, we propose an attention-based neural network model for joint intent detection and slot filling, both of which are critical steps for many speech understanding and dialog systems. Unlike in machine translation and speech recognition, alignment is explicit in slot filling. We explore different strategies in incorporating this alignment information to the encoder-decoder framework. Learning from the attention mechanism in encoder-decoder model, we further propose introducing attention to the alignment-based RNN models. Such attentions provide additional information to the intent classification and slot label prediction. Our independent task models achieve state-of-the-art intent detection error rate and slot filling F1 score on the benchmark ATIS task. Our joint training model further obtains 0.56% absolute (23.8% relative) error reduction on intent detection and 0.23% absolute gain on slot filling over the independent task models.
Building diverse computer systems (2002)

Diversity is an important source of robustness in biological systems. Computers, by contrast, are notable for their lack of diversity. Although homogeneous systems have many advantages, the beneficial effects of diversity in computing systems have been overlooked, specifically in the area of computer security. Several methods of achieving software diversity are discussed based on randomizations that respect the specified behavior of the program. Such randomization could potentially increase the robustness of software systems with minimal impact on convenience, usability, and efficiency. Randomization of the amount of memory allocated on a stack frame is shown to disrupt a simple buffer overflow attack.
Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules (2018)

Large-scale manufacturing using modular systems and semi-automated devices resulted in highly functional clinical-grade TCR transduced T-cells. This process is now in use in actively accruing clinical trials and the NIH Clinical Center and can be utilized at other cell therapy manufacturing sites that wish to scale-up and optimize their processing using closed systems.
Multimode interference coupler based photonic analog-to-digital conversion scheme (2012)

A novel phase-shifted optical quantization scheme for an all-optical analog-to-digital converter, which uses 4×4 multimode interference couplers, is demonstrated and theoretically analyzed. The whole scheme can be integrated on a chip.
High efficiency 3 W side-pumped Yb fiber amplifier and laser (2003)

Summary form only given. We describe a Yb-doped double cladding fibre (DCF) amplifier, which when pumped by a 104 /spl mu/m wide broad stripe laser diode generates 3.3 W and exhibits a maximum electrical-to-optical conversion efficiency of 34 percent. The amplifier employs v-groove side-pumping which allows compact amplifier packaging.
RNAi Screen Indicates Widespread Biological Function for Human Natural Antisense Transcripts (2010)

Natural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a loss-of-function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.
Regulation of alcohol fermentation in Escherichia coli. Progress report, July 1991--June 1994 (1994)

approximately 2700bp of DNA for its coding sequence. We have also isolated mutations affecting the fermentative lactate dehydrogenase and have recently cloned the ldh gene. In consequence it is now possible to construct E. coli strains defective in the production of any one or more of their normal fermentation products (i.e. formate, acetate, lactate, ethanol and succinate). The factors affecting ratio of fermentation products are being investigated by in vivo NMR spectroscopy.
Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach (2000)

We propose a rational approach to the generation of live viral vaccines: alteration of virally encoded type I IFN antagonists to attenuate virulence while retaining immunogenicity. We have explored this concept by using the influenza virus. Previously we have shown that the NS1 protein of influenza A virus possesses anti-IFN activity. We now present evidence that influenza A and B viruses encoding altered viral NS1 proteins are highly attenuated in the mouse host, yet provide protection from challenge with wild-type viruses.
Analytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresis (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresisA. Guttman, A. S. Cohen, D. N. Heiger, and Barry L. KargerCite this: Anal. Chem. 1990, 62, 2, 137–141Publication Date (Print):January 15, 1990Publication History Published online1 May 2002Published inissue 15 January 1990https://pubs.acs.org/doi/10.1021/ac00201a010https://doi.org/10.1021/ac00201a010research-articleACS PublicationsRequest reuse permissionsArticle Views356Altmetric-Citations218LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
In Vitro Effects of Acute Amiodarone and Dronedarone on Epicardial, Endocardial, and M Cells of the Canine Ventricle (2007)

Amiodarone (AM) is an antiarrhythmic agent widely used in the treatment of ventricular and supraventricular arrhythmias. Dronedarone (DR) is a new compound with a pharmacological profile similar to that of AM, but iodine free. We previously demonstrated that chronic AM treatment reduces transmural dispersion of repolarization (TDR) in the canine heart. We used standard microelectrode technique to evaluate the effects of acute AM (100 microM) and DR (30 microM) on epicardial (EPI), endocardial (ENDO), and M region tissues obtained from the left ventricular wall of the canine heart. Amiodarone (100 microM, 120 min of exposure) produced little change in the action potential duration of ENDO and EPI tissues, but it shortened the action potential of M cells, especially at slow rates, leading to a decrease in TDR. Similar results were observed with DR. Acute AM (100 microM) and DR (30 microM) eliminated d-sotalol-induced early afterdepolarizations (EADs) and triggered activity in 3 of 3 and 2 of 6 M cell preparations, respectively. The reduction of TDR and the elimination of EAD-induced triggered activity differentiates AM and DR from other class III agents. These effects may explain the efficacy and low arrhythmogenicity of acute AM and suggest a potential safe use of DR as an antiarrhythmic agent.
Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis (2020)

Despite signs of infection, the involvement of the oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, and 15 immune cell clusters. Analysis of SARS-CoV-2 viral entry factor expression showed enrichment in epithelia including the ducts and acini of the salivary glands and the suprabasal cells of the mucosae. COVID-19 autopsy tissues confirmed in vivo SARS-CoV-2 infection in the salivary glands and mucosa. Saliva from SARS-CoV-2-infected individuals harbored epithelial cells exhibiting <i>ACE2</i> expression and SARS-CoV-2 RNA. Matched nasopharyngeal and saliva samples found distinct viral shedding dynamics and viral burden in saliva correlated with COVID-19 symptoms including taste loss. Upon recovery, this cohort exhibited salivary antibodies against SARS-CoV-2 proteins. Collectively, the oral cavity represents a robust site for COVID-19 infection and implicates saliva in viral transmission.
Role of Citrate Synthase in Aldosterone-Mediated Sodium Reabsorption (2000)

Aldosterone and other mineralocorticoids increase citrate synthase activity in the kidney and enhance renal sodium reabsorption, but it is unclear whether the increased citrate synthase activity is involved in renal sodium transport. We used the Wistar-Furth rat, an inbred strain found to be deficient in renal citrate synthase activity, as an experimental model to investigate this issue. We confirmed that renal citrate synthase activity from adrenalectomized Wistar-Furth rats was decreased compared with that from control Wistar rats (by 28%). Similarly, urinary citrate excretion was 23% lower in Wistar-Furth rats. Subnormal citrate formation in Wistar-Furth rats could not be accounted for by differences in systemic pH or circulating potassium levels. Because renal citrate synthase activity was reduced in Wistar-Furth rats, we hypothesized that renal sodium excretory responses to mineralocorticoids would be reduced as well. Four-hour sodium excretion after intraperitoneal injection of 5 microg of aldosterone was reduced by 56% in adrenalectomized Wistar rats and by 52% in adrenalectomized Wistar-Furth rats (both P<0.01 compared with vehicle injection). Similarly, the pattern of urinary sodium excretion in response to subcutaneous injections of deoxycorticosterone acetate over a 2-week period was similar in adrenalectomized Wistar and Wistar-Furth rats. In summary, acute and chronic antinatriuretic responses to mineralocorticoids are maintained in Wistar-Furth rats at the level of Wistar rats, despite the marked reduction in citrate synthase activity. These findings are not consistent with an important role for citrate synthase activity in mineralocorticoid-mediated renal sodium transport.
Human immortalized brown adipocytes express functional β3-adrenoceptor coupled to lipolysis (1997)

Human brown pre-adipocytes were immortalized by microinjection of the genes encoding simian virus 40 T and t antigens under the control of the human vimentin promotor. The transfected pre-adipocytes were cultured for several months with no loss of their morphological characteristics. These cells accumulate lipids and differentiate into adipocytes when treated with insulin, triiodothyronine and dexamethazone. The mRNA of various adipocyte markers was detected by reverse transcriptase-polymerase chain reaction analysis, including hormone-sensitive lipase, lipoprotein lipase, adipsin, glucose transporters 1 and 4, the uncoupling protein (specific of brown adipocytes), and leptin, the product of the ob gene. Pharmacological analyses indicated that the beta3-adrenoceptor is the predominant beta-adrenoceptor subtype in PAZ6 cells and that this receptor subtype is functionally coupled to adenylate cyclase and lipolysis. The immortalization of human adipocytes will permit pharmacological analysis of the human beta3-adrenoceptor function in adipose cells and will allow detailed studies of human adipocyte differentiation.
Stem cell technology for tendon regeneration: current status, challenges, and future research directions (2015)

Tendon injuries are a common cause of physical disability. They present a clinical challenge to orthopedic surgeons because injured tendons respond poorly to current treatments without tissue regeneration and the time required for rehabilitation is long. New treatment options are required. Stem cell-based therapies offer great potential to promote tendon regeneration due to their high proliferative, synthetic, and immunomodulatory activities as well as their potential to differentiate to the target cell types and undergo genetic modification. In this review, I first recapped the challenges of tendon repair by reviewing the anatomy of tendon. Next, I discussed the advantages and limitations of using different types of stem cells compared to terminally differentiated cells for tendon tissue engineering. The safety and efficacy of application of stem cells and their modified counterparts for tendon tissue engineering were then summarized after a systematic literature search in PubMed. The challenges and future research directions to enhance, optimize, and standardize stem cell-based therapies for augmenting tendon repair were then discussed.
Highly Selective Phosphatidylinositol 4-Kinase IIIβ Inhibitors and Structural Insight into Their Mode of Action (2015)

Phosphatidylinositol 4-kinase IIIβ is a cellular lipid kinase pivotal to pathogenesis of various RNA viruses. These viruses hijack the enzyme in order to modify the structure of intracellular membranes and use them for the construction of functional replication machinery. Selective inhibitors of this enzyme are potential broad-spectrum antiviral agents, as inhibition of this enzyme results in the arrest of replication of PI4K IIIβ-dependent viruses. Herein, we report a detailed study of novel selective inhibitors of PI4K IIIβ, which exert antiviral activity against a panel of single-stranded positive-sense RNA viruses. Our crystallographic data show that the inhibitors occupy the binding site for the adenine ring of the ATP molecule and therefore prevent the phosphorylation reaction.
Analysis of rib dielectric waveguides (1985)

An analysis of rib dielectric waveguides based on a mode matching technique is presented. When the constituent slab guides support only one guided mode, it is shown that the cutoff condition for the higher order modes is the same as the result of the effective dielectric constant method. However, when the rib region is thick enough to support two guided slab modes, the cutoff conditions are significantly different. For this case universal design curves are produced and plotted. The results are also compared with another theory that exists to analyze such structures.
Out-of-plane properties (1995)

This paper summarizes three areas of research which were performed to characterize out-of-plane properties of composite materials. In the first investigation, a series of tests was run to characterize the through-the-thickness tensile strength for a variety of composites that included 2D braids, 2D and 3D weaves, and prepreg tapes. A new test method based on a curved beam was evaluated. Failures were significantly different between the 2D materials and the 3D weaves. The 2D materials delaminated between layers due to out-of-plane tensile stresses while the 3D weaves failed due to the formation of radial cracks between the surface plies caused by high circumferential stresses along the inner radius. The strength of the 2D textile composites did not increase relative to the tapes. Final failure in the 3D weaves was caused by a circumferential crack similar to the 2D materials and occurred at a lower bending moment than in other materials. The early failures in the 3D weaves were caused by radial crack formation rather than a low through-the-thickness strength. The second investigation focused on the development of a standard impact test method to measure impact damage resistance. The only impact tests that currently exist are compression after impact (CAI) tests which incorporate elements of both damage resistance and damage tolerance. A new impact test method is under development which uses a quasi-static indentation (QSI) test to directly measure damage resistance. Damage resistance is quantified in terms of the contact force to produce a unit of damage where a metric for damage may be area in C-scan, depth of residual dent , penetration, damage growth, etc. A final draft of an impact standard that uses a QSI test method will be presented to the ASTM Impact Task Group on impact. In the third investigation, the impact damage resistance behavior of a variety of textile materials was studied using the QSI test method. In this study, the force where large damage initiates was measured and the delamination size as a function of force was determined. The force to initiate large damage was significantly lower in braids and weaves. The delamination diameter - impact forace relationship was quanitfied using a damage resistance parameter, Q(*), which related delamination diameter to imapct force over a range of delamination sizes. Using this Q(*) parameter to rate the materials, the stitched uniweaves, toughened epoxy tapes, and through-the-thickness orthogonal interlock weave were the most damage resistant.
Brain systems for encoding and retrieval of auditory—verbal memory. An in vivo study in humans (1995)

Long-term auditory-verbal memory comprises, at a neuropsychological level, a number of distinct cognitive processes. In the present study we determined the brain systems engaged during encoding (experiment 1) and retrieval (experiment 2) of episodic auditory-verbal material. In the separate experiments, PET measurements of regional cerebral blood flow (rCBF), an index of neural activity, were performed in normal volunteers during either the encoding or the retrieval of paired word associates. In experiment 1, a dual task interference paradigm was used to isolate areas involved in episodic encoding from those which would be concurrently activated by other cognitive processes associated with the presentation of paired associates, notably priming. In experiment 2, we used the cued retrieval of paired associates from episodic or from semantic memory in order to isolate the neural correlates of episodic memories. Encoding of episodic memory was associated with activation of the left prefrontal cortex and the retrosplenial area of the cingulate cortex, while retrieval from episodic memory was associated with activation of the precuneus bilaterally and of the right prefrontal cortex. These results are compatible with the patterns of activation reported in a previous PET memory experiment in which encoding and retrieval were studied concurrently. They also indicate that separate brain systems are engaged during the encoding and retrieval phases of episodic auditory-verbal memory. Retrieval from episodic memory engages a different, but overlapping, system to that engaged by retrieval from semantic memory, a finding that lends functional anatomical support to this neuropsychological distinction.
PRICING AND CYBERSALES (1998)

SUBTITLE: THE RAPID GROWTH OF DIRECT INTERNET RESERVATIONS IS FORCING AIRLINE YIELD MANAGERS BACK TO THE DRAWING BOARD.
The Effects of Rat Mesenchymal Stem Cells on Injury Progression In a Rat Model (2013)

Intravenous injection of rat MSCs reduced burn injury progression in a rat comb-burn model.
Export finance - issues and directions : case study of the Philippines (1990)

In sequencing policy reforms in developing countries, governments should assign a high priority to measures that assure rational regimes for export activities. For policy reforms to have a significant impact on growth, foreign demand for export products must be matched by active supply responses from exporters. External demand for export products takes the form of letters of credit (L/C), that is, payment for export orders is guaranteed by the importers' banks. Thus, an export L/C amounts to a sales agreement. But without access to financing needed to fill export orders, exporters' supply response to foreign demands cannot be realized. The focus of the study was to identify factors constraining access to export finance at reasonable cost. It is based on a survey of exporters and commercial bankers, geared to providing an empirical and analytical basis for reforming the export financing system of the Philippines. The study provides a summary of the findings and the characteristics of trade finance market mechanisms. The export finance systems of the Central Bank and other government agencies and preshipment export guarantee schemes are reviewed. Interview results on export loan access problems are presented and the need for creating a foreign currency revolving fund is discussed. It also includes a discussion on the need for reforming the preshipment export finance mechanisms and extending coverage to indirect exports and the role of export credit insurance/guarantee.
The BET bromodomain inhibitor JQ1 activates HIV latency through antagonizing Brd4 inhibition of Tat-transactivation (2012)

Latent HIV reservoirs are the primary hurdle to eradication of infection. Identification of agents, pathways and molecular mechanisms that activate latent provirus may, in the presence of highly active antiretroviral therapy, permit clearance of infected cells by the immune system. Promoter-proximal pausing of RNA polymerase (Pol) II is a major rate-limiting step in HIV gene expression. The viral Tat protein recruits human Super Elongation Complex (SEC) to paused Pol II to overcome this limitation. Here, we identify the bromodomain protein Brd4 and its inhibition of Tat-transactivation as a major impediment to latency reactivation. Brd4 competitively blocks the Tat-SEC interaction on HIV promoter. The BET bromodomain inhibitor JQ1 dissociates Brd4 from the HIV promoter to allow Tat recruitment of SEC to stimulate HIV elongation. JQ1 synergizes with another latency activator prostratin, which promotes Pol II loading onto the viral promoter. Because JQ1 activates viral latency without inducing global T cell activation, this and other closely related compounds and their antagonization of Brd4 to promote Tat-SEC interaction merit further investigations as effective agents/strategies for eliminating latent HIV.