gebhart/patrepeval huggingface dataset:
Correlative Neuroanatomy & Functional Neurology (1982)

Correlative neuroanatomy & Functional Neurology , Correlative neuroanatomy & Functional Neurology , کتابخانه دیجیتال جندی شاپور اهواز
Histone deacetylase inhibitors: A new mode for inhibition of cholesterol metabolism (2008)

Our results provide new insights in the effects of histone deacetylases on genes involved in primary metabolism. This observation suggests that TSA, and other related histone deacetylase inhibitors, may be useful as potential therapeutic entities for the control of cholesterol levels in humans.
Cascaded pose regression (2010)

We present a fast and accurate algorithm for computing the 2D pose of objects in images called cascaded pose regression (CPR). CPR progressively refines a loosely specified initial guess, where each refinement is carried out by a different regressor. Each regressor performs simple image measurements that are dependent on the output of the previous regressors; the entire system is automatically learned from human annotated training examples. CPR is not restricted to rigid transformations: `pose' is any parameterized variation of the object's appearance such as the degrees of freedom of deformable and articulated objects. We compare CPR against both standard regression techniques and human performance (computed from redundant human annotations). Experiments on three diverse datasets (mice, faces, fish) suggest CPR is fast (2-3ms per pose estimate), accurate (approaching human performance), and easy to train from small amounts of labeled data.
Fluoxetine depresses glutamate exocytosis in the rat cerebrocortical nerve terminals (synaptosomes) via inhibition of P/Q‐type Ca2+ channels (2003)

Fluoxetine, an antidepressant that is used clinically in the treatment of mood disorders, is a selective serotonin reuptake inhibitor. In the present study we investigated the effects of fluoxetine on 4-aminopyridine (4AP)-evoked glutamate release in cerebrocortical nerve terminals (synaptosomes). Fluoxetine suppressed the release of glutamate evoked by 4AP in a concentration-dependent manner. This effect was associated with a reduction in the depolarization-evoked increase in cytosolic free calcium levels in the absence of significant effect on the synaptosomal membrane potential. In addition, both ionomycin- and sucrose-evoked glutamate releases were not affected by fluoxetine, indicating that fluoxetine-mediated inhibition of glutamate release is not a direct effect on the exocytotic machinery. Furthermore, the inhibitory action of fluoxetine was completely abolished in synaptosomes pretreated with P/Q type Ca(2+) channel blocker omega-agatoxin IVA (omega-AgTX IVA) or protein kinase C (PKC) stimulator 4beta-phorbol 12, 13-dibutyrate (PDBu). These results suggest that, in cerebrocortical nerve terminals, fluoxetine inhibits glutamate release through the suppression of P/Q type Ca(2+) channel activity. The presynaptic action of fluoxetine is mediated by a PKC-sensitive signaling pathway. Synapse 48:170-177, 2003.
Expression of frizzled‐related protein and Wnt‐signalling molecules in invasive human breast tumours (2001)

Frizzled-related protein (Frp) is a new family of secreted proteins that contain a region homologous to the extracellular cysteine-rich domain (CRD) of the frizzled family proteins. The role of Frp protein is far from clear. To explore the role of Frp and its relationship to the Wnt-signalling pathway in breast cancer, in situ hybridization and immunohistochemical analyses of Frp, Wnt-1, APC, beta-catenin, and its target genes c-myc and cyclin D1 were conducted in 70 specimens of invasive ductal carcinomas of the human breast. Frp mRNA was down-regulated in 62 and elevated in eight tumour specimens, compared with adjacent normal tissues. In the course of tumour progression, however, Frp mRNA steadily increased in both tumour and the adjacent tissues. Interestingly, the number of cases with axillary lymph node metastasis was significantly lower in the group with elevated Frp than in the group with decreased Frp, suggesting that Frp may contribute as a prognostic factor in invasive breast cancer. Wnt-1, a gene implicated in human breast cancer, was markedly elevated in grade 1 tumours, but declined as tumour grade declined. The level of Wnt-1 was linearly correlated with its downstream target beta-catenin (p<0.05), but was inversely correlated with Frp (p<0.05), suggesting a possible negative regulatory role of Frp with regard to Wnt-1. APC was inversely correlated with beta-catenin (p<0.05). Beta-catenin, a key transcriptional activator responsible for the activation of both c-myc and cyclin D1 in colorectal tumours, was detected at high levels in the plasma membranes of cells in normal tissue. In tumour masses, however, beta-catenin lost its tight association with the membrane and diffused into the cytoplasm. Surprisingly, it clearly did not penetrate the nuclei, despite the fact that both c-myc and cyclin D1 were markedly elevated in all tumour tissues. As revealed in this study, Wnt-1/beta-catenin plays very different roles in the oncogenesis of breast and colon cancers. This first systemic analysis of the Frp and the Wnt-signalling pathway in human breast cancer provides a springboard for further work on the role of Frp in the development of breast cancer.
Cost and Quality-of-Service-aware Network Service Deployment. (2001)

This paper presents the information aggregation methods enabling cost- and QoS-aware service deployment. It is expected that network management tools will require the network itself to participate in the service deployment task so as to adapt to heterogeneous networknodes cost, QoS, and capabilities. The aggregation methods are part of a set of hierarchically-distributed computations, for which a formal description is presented. Four types of information that can be handled by the mechanism are introduced and illustrated by examples from three service-deployment categories.
MOS /metal oxide semiconductor/ physics and technology (1982)

Introduction. Field Effect. Metal Oxide Silicon Capacitor at Low Frequencies. Metal Oxide Silicon Capacitor at Intermediate and High Frequencies. Extraction of Interface Trap Properties from the Conductance. Interfacial Nonuniformities. Experimental Evidence for Interface Trap Properties. Extraction of Interface Trap Properties from the Capacitance. Measurement of Silicon Properties. Charges, Barrier Heights, and Flatband Voltage. Charge Trapping in the Oxide. Instrumentation for Measuring Capacitor Characteristics. Oxidation of Silicon--Oxidation Kinetics. Oxidation of Silicon--Technology. Control of Oxide Charges. Models of the Interface. Appendices. Subject Index. Symbol Index.
Anti-tumor activity of CC49-doxorubicin immunoconjugates. (1995)

The TAG72 reactive monoclonal antibody CC49 was conjugated to doxorubicin with a malonate linker. The immunoconjugate, designated CC49-BAMME-CH-DOX, was approximately a log less potent than unconjugated doxorubicin in an in vitro cytotoxicity assay. Immunoreactivity of the antibody was fully retained. When evaluated in a nude mouse xenograft model with the antigen positive LS174T human colorectal tumor target, CC49-BAMME-CH-DOX and free doxorubicin had similar tumor suppressive activities. The immunoconjugate was clearly less toxic, however, as measured by weight loss and deaths. When evaluated in an NIH:OVCAR-3 human ovarian carcinoma xenograft model, CC49-BAMME-CH-DOX was superior at prolonging survival in comparison to free doxorubicin, unmodified CC49, and a non tumor binding doxorubicin immunoconjugate. These results indicate that targeting of doxorubicin with the CC49 antibody can improve the toxicity and/or the potency of the drug, depending on the tumor target being evaluated. CC49-Doxorubicin immunoconjugates should be considered for clinical evaluation.
Immunopathologic features of Staphylococcus aureus endophthalmitis in the rat. (1995)

S. aureus endophthalmitis is associated with the infiltration of neutrophils, lymphocytes, monocytes, and plasma cells in vitreous. Neutrophils, the predominant infiltrating cells, may be involved in bactericidal activity and opsonophagocytosis. In rat staphylococcal endophthalmitis, IgM rather than IgG may be the protective antibody.
Dictyostelium cytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro (1996)

A fucosyltransferase activity has been detected using lacto-N-biose I as acceptor in the lower eukaryote Dictyostelium discoideum. This transferase requires divalent cations and is inhibited by N-ethylmaleimide and detergent treatment. Apparent calculated Km values for GDP-Fuc and lacto-N-biose I are 1.27 microM and 2.80 mM, respectively. The activity is quantitatively recovered in the supernatant after centrifugation at 100000 x g for 1 h. The reaction product, as determined by gel permeation chromatography, sensitivity to fucosidases, and analysis of partially methylated derivatives, is Fucalpha1-2Galbeta1-3GlcNAc (H type 1 trisaccharide).
Uniform, highly conductive, and patterned transparent films of a percolating silver nanowire network on rigid and flexible substrates using a dry transfer technique (2010)

Silver nanowire films are promising alternatives to tin-doped indium oxide (ITO) films as transparent conductive electrodes. In this paper, we report the use of vacuum filtration and a polydimethylsiloxane (PDMS)-assisted transfer printing technique to fabricate silver nanowire films on both rigid and flexible substrates, bringing advantages such as the capability of patterned transfer, the best performance among various ITO alternatives (10 Ω/sq at 85% transparency), and good adhesion to the underlying substrate, thus eliminating the previously reported adhesion problem. In addition, our method also allows the preparation of high quality patterned films of silver nanowires with different line widths and shapes in a matter of few minutes, making it a scalable process. Furthermore, use of an anodized aluminum oxide (AAO) membrane in the transfer process allows annealing of nanowire films at moderately high temperature to obtain films with extremely high conductivity and good transparency. Using this transfer technique, we obtained silver nanowire films on a flexible polyethylene terephthalate (PET) substrate with a transparency of 85%, a sheet resistance of 10 Ω/sq, with good mechanical flexibility. Detailed analysis revealed that the Ag nanowire network exhibits two-dimensional percolation behavior with good agreement between experimentally observed and theoretically predicted values of critical volume.
Chromatographic resolution of organic acids using the Kromasil‐CHI‐TBB chiral stationary phase (1999)

Derivatives of racemic mandelic and tropic acids have been separated directly on a t-butylbenzoylated tartaric acid-based chiral stationary phase (CSP) (Kromasil-CHI-TBB) on both analytical and preparative scales. The resolution of the enantiomers of the model compound, rac-3-methoxytropic acid, was optimized by changing the nature and the composition of the mobile phase. The dominating interaction between the CSP and these hydroxy acids was found to be hydrogen-bonding. Consequently, retention of the analytes on the CSP decreased with increasing polarity of the mobile phase modifier. The separation of the enantiomers was strongly influenced by the π-donating and π-accepting ability of the aryl moiety, and strong electron-withdrawing substituents in the aryl group e.g.,—CF3 result in a loss of chiral recognition. However, the CSP can discriminate between the two enantiomers only when both the aryl moiety and the carboxylic function are directly bonded to the asymmetric carbon atom. The Kromasil-CHI-TBB column displays broad selectivity for the enantiomers of mandelic and tropic acid derivatives, which are relatively simple structures and not easily resolved. Even though the α-values are moderate, the high efficiency of the column facilitates the use of the CSP for the preparative scale chiral separation of a number of these compounds. Chirality 11:420–425, 1999. © 1999 Wiley-Liss, Inc.
Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a (2000)

Current evidence indicates that methylation of cytosine in mammalian DNA is restricted to both strands of the symmetrical sequence CpG, although there have been sporadic reports that sequences other than CpG may also be methylated. We have used a dual-labeling nearest neighbor technique and bisulphite genomic sequencing methods to investigate the nearest neighbors of 5-methylcytosine residues in mammalian DNA. We find that embryonic stem cells, but not somatic tissues, have significant cytosine-5 methylation at CpA and, to a lesser extent, at CpT. As the expression of the de novo methyltransferase Dnmt3a correlates well with the presence of non-CpG methylation, we asked whether Dnmt3a might be responsible for this modification. Analysis of genomic methylation in transgenic Drosophila expressing Dnmt3a reveals that Dnmt3a is predominantly a CpG methylase but also is able to induce methylation at CpA and at CpT.
Redox deracemization of 1,3,4,9-tetrahydropyrano[3,4-b]indoles (2018)

The existing asymmetric synthesis of enantiopure α-substituted cyclic ethers predominantly relies on the enantioselective C-C bond formation involving a prochiral oxocarbenium ion intermediate. In such a strategy, enantioselectivity and efficiency are typically susceptible to the electronic and substituent effects of either nucleophile or electrophile partners. Here, we describe a strategically different redox deracemization of α-substituted 1,3,4,9-tetrahydropyrano[3,4-b]indoles. This method exhibits good compatibility with the regional variation of the electronic or substituent effect of substrates, thus providing a practical and efficient supplement to the traditional strategy.
Application of laser capture microdissection to cytologic specimens for the detection of immunoglobulin heavy chain gene rearrangement in patients with malignant lymphoma (2003)

The results of the current study suggest that the novel application of LCM to cytologic specimens occasionally exhibits high sensitivity for the detection of IgH gene rearrangement monoclonality compared with the use of histologic sections.
Protective effect of non-selective and selective COX-2-inhibitors in acute immobilization stress-induced behavioral and biochemical alterations. (2008)

Acute stress has been known to produce several behavioral, neurochemical and biochemical alterations. Cyclooxygenase (COX) enzymes are involved in pathogenesis of several brain disorders including Alzheimer disease, epilepsy, depression, in addition to pain and inflammation. In the present study, we examined the role of non-selective (naproxen) and selective (rofecoxib, valdecoxib) COX-2 inhibitors against acute immobilization stress-induced behavioral alterations and oxidative damage in mice. Mice were subjected to acute immobilization stress for a period of 6 h. Naproxen (7 and 14 mg/kg, ip), rofecoxib (5 and 10 mg/kg, ip) or valdecoxib (5 and 10 mg/kg, ip) were administered 30 min before acute stress. Six-our immobilization stress significantly caused anxiety-like behavior, memory deficit and impaired motor activity as well as oxidative damage (raised lipid peroxidation, nitrite activity, depletion of reduced glutathione and catalase activity) as compared to naive animals placed on sawdust (p < 0.05). Pretreatment with naproxen (7 and 14 mg/kg, ip), rofecoxib (5 and 10 mg/kg, ip) and valdecoxib (5 and 10 mg/kg, ip) significantly improved locomotor activity, antianxiety effect, memory retention (memory deficit) and attenuated oxidative damage (lowering of raised malondialdehyde, nitrite activity, restoration of reduced glutathione and catalase activity as compared to immobilization stress group (p < 0.05). Results suggest the neuroprotective and antioxidant effect of both non-selective and selective COX-2 inhibitors.
Preparations of perhydroimidazo[1,5-a]pyridine-3-thiones and thiazole-5-spirocyclopropan-4(5H)-ones from thioureas and α,γ-dibromobutyryl chloride under phase-transfer conditions (1986)

The reaction of N-monosubstituted thioureas (1) with α,γ-dibromobutyryl chloride (2) was carried out in 5% NaOH–CH2Cl2 to afford 2-alkyl(aryl)-6-bromo-2,3,7,8-tetrahydro-3-thioxo-6,8a-ethano-imidazo[1,5-a]pyridine-1,5(6H,8aH)-diones (3) and 2-alkyl(aryl)-aminothiazole-5-spirocyclopropan-4(5H)-ones (5).
A Secondary Gate As a Mechanism for Inhibition of the M2 Proton Channel by Amantadine (2008)

The mechanism of inhibition of the influenza A virus M2 proton channel by the antiviral drug amantadine has been under intense investigation. The importance of a mechanistic understanding is heightened by the prevalence of amantadine-resistant mutations. To gain mechanistic insight at the molecular level, we carried out extensive molecular dynamics simulations of the tetrameric M2 proton channel in both apo and amantadine-bound forms in a lipid bilayer. The simulation of the apo form revealed that Val27 from the four M2 subunits can form a secondary gate near the channel entrance and break the water wire in the channel pore. This gate arises from physical occlusion and the elimination of hydrogen-bonding partners for water molecules. In the presence of amantadine, the secondary gate formed by Val27 and the drug molecule lying just below form an extended blockage, which breaks the water wire throughout the simulation. The location and orientation of amantadine inside of the channel pore as found in our simulation are supported by a host of experimental observations. Our study suggests a novel role for Val27 in the inhibition of the M2 proton channel by amantadine.
Quantum Gaussian noise (2003)

In semiclassical theory, light is a classical electromagnetic wave and the fundamental source of photodetection noise is the shot effect arising from the discreteness of the electron charge. In quantum theory, light is a quantum-mechanical entity and the fundamental source of photodetection noise comes from measuring the photon-flux operator. The Glauber coherent states are Gaussian quantum states which represent classical electromagnetic radiation. Quantum photodetection of these states yields statistics that are indistinguishable from the corresponding Poisson point-process results of semiclassical photodetection. Optical parametric interactions, however, can be used to produce other Gaussian quantum states, states whose photodetection behavior cannot be characterized semiclassically. A unified analytical framework is presented for Gaussian-state photodetection that includes the full panoply of nonclassical effects that have been produced via parametric interactions.
Northern blot normalization with a 28S rRNA olinucleotide probe (1989)

Journal Article Northern blot normalization with a 28S rRNA olinucleotide probe Get access V. Barbu, V. Barbu Search for other works by this author on: Oxford Academic PubMed Google Scholar F. Dautry F. Dautry 1Laboratoire d'Oncologie Moléculaire, Institut Gustave Roussy94805 Villejuif Cadex, France Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 17, Issue 17, 11 September 1989, Page 7115, https://doi.org/10.1093/nar/17.17.7115 Published: 11 September 1989 Article history Received: 25 July 1989 Published: 11 September 1989
Expression of a novel human sialidase encoded by the NEU2 gene (1999)

Sialidases (E.C.3.2.1.18) belong to a group of glycohydrolytic enzymes, widely distributed in nature, which remove sialic acid residues from glycoproteins and glycolipids. All of the sialidase so far characterized at the molecular level share an Asp block, repeated three to five times in the primary structure, and an F/YRIP sequence motif which is part of the active site. Using a sequence homology-based approach, we previously identified a human gene, named NEU2, mapping to chromosome 2q37. NEU2 encoded protein is a polypeptide of 380 amino acids with two Asp block consensuses and the YRIP sequence in the amino terminal part of the primary structure. Here we demonstrate that NEU2 encodes a functional sialidase. NEU2 was expressed in COS7 cells, giving rise to a dramatic increase in the sialidase activity measured in cell extracts with the artificial substrate 4-MU-NANA. Using a rabbit polyclonal antiserum, on Western blots a protein band with a molecular weight of about 42 kDa was detectable, and its cytosolic localization was demonstrated with cell fractionation experiments. These results were confirmed using immunohistochemical techniques. NEU2 expression in E.coli cells allowed purification of the recombinant protein. As already observed in the enzyme expressed in COS7 cells, NEU2 pH optimum corresponds to 5.6 and the polypeptide showed a K(m)for 4-MU-NANA of 0.07 mM. In addition, based on the detectable similarities between the NEU2 amino acid sequence and bacterial sialidases, a prediction of the three-dimensional structure of the enzyme was carried out using a protein homology modeling approach.
Measurement of the Lactate Dehydrogenase Activity of Plasmodium falciparum as an Assessment of Parasitemia (1993)

This report describes an enzyme assay for the detection of Plasmodium falciparum. The assay is based on the observation that the lactate dehydrogenase (LDH) enzyme of P. falciparum has the ability to rapidly use 3-acetyl pyridine NAD (APAD) as a coenzyme in the reaction leading to the formation of pyruvate from lactate. Human red blood cell LDH carries out this reaction at a very slow rate in the presence of APAD. We measured the development of APADH and found that the formation of this product could establish the basis of an assay that detected the presence of P. falciparum from in vitro cultures at parasitemia levels of 0.02%. We also had occasion to use this assay with clinical samples. We found a correlation between levels of parasitemia and the activity of parasite LDH. Parasite LDH (pLDH) activity could be measured in blood hemolysates and in plasma and serum from patients with malaria. We used the serum assay for pLDH and followed the level of pLDH in a patient with cerebral malaria prior to antimalarial treatment and during the recovery period. From these initial studies, it is evident that the measurement of pLDH has a correlation with parasitemia and may offer a method that can be developed into a simple test for the detection of Plasmodium parasitemia.
Daily-deal selection for revenue maximization (2012)

Daily-Deal Sites (DDS) like Groupon, LivingSocial, Amazon's Goldbox, and many more, have become particularly popular over the last three years, providing discounted offers to customers for restaurants, ticketed events, services etc. In this paper, we study the following problem: among a set of candidate deals, which are the ones that a DDS should feature as daily-deals in order to maximize its revenue? Our first contribution lies in providing two combinatorial formulations of this problem. Both formulations take into account factors like the diversification of daily deals and the limited consuming capacity of the userbase. We prove that our problems are NP-hard and devise pseudopolynomial -- time approximation algorithms for their solution. We also propose a set of heuristics, and demonstrate their efficiency in our experiments. In the context of deal selection and scheduling, we acknowledge the importance of the ability to estimate the expected revenue of a candidate deal. We explore the nature of this task in the context of real data, and propose a framework for revenue-estimation. We demonstrate the effectiveness of our entire methodology in an experimental evaluation on a large dataset of daily-deals from Groupon.