gebhart/patrepeval huggingface dataset:
Inactivation of the Human Immunodeficiency Virus by Hypericin: Evidence for Photochemical Alterations of p24 and a Block in Uncoating (1992)

Following attachment and entry of human immunodeficiency virus (HIV) into a host cell, the HIV genomic RNA is reverse transcribed to cDNA. This step may be inhibited by hypericin, a compound that induces alterations of the retroviral capsid. Incubation of HIV with hypericin rendered the virus noninfectious. The replication of HIV was blocked early; HIV cDNA could not be detected in cells challenged with hypericin-treated HIV. Hypericin did not inhibit the binding of recombinant gp120 to CD4+ cells, nor did hypericin inhibit syncytium formation. However, reverse transcriptase activity could not be released from hypericin-treated virions. Western blot analysis revealed altered mobility of the HIV major capsid protein (p24) following hypericin treatment. Hypericin-treated recombinant HIV p24 exhibited similar altered mobility. The inactivation of HIV infectivity and the alterations in p24 mobility required hypericin incubations in the presence of visible light. Collectively, these data suggest that photochemical alterations of the HIV capsid may contribute to the hypericin-mediated inactivation of HIV. Such alterations may inhibit the release of RT activity from treated HIV, and prevent uncoating and subsequent reverse transcription of the HIV genome within a target cell.
Synthesis, Evaluation, and Formulation Studies on New Oxidizers as Alternatives to Ammonium Perchlorate in DoD Missile Propulsion Applications (2007)

Abstract : Perchlorate is found in groundwater and drinking water throughout the United States. This contamination is primarily attributed to the use of ammonium perchlorate in the solid fuel for rockets and missiles. The objective of the program is to develop environmentally benign solid rocket propellant formulations that do not rely on the use of ammonium perchlorate (AP) as an oxidizer. This objective supports the goal of reducing future AP contamination in groundwater by reducing the need for production and use of AP as an oxidizer in solid rocket motors. The propellants developed must match current performance and hazards to meet the objective. To formulate propellants that don't rely on AP, combinations of oxidizers must be exploited. We selected four new supplemental oxidizers to focus on initially. These include the inorganic oxidizer ammonium di(nitramido) amine (ADNA); the cyclic nitramine/gem-dinitro compounds such as 1,3,5,5-tetranitrohexahydropyrimidine (DNNC) and 1,3,3,5,7,7-hexanitro-1,5- diazacyclooctane (HCO) and by adding the dinitroethylene attachment to nitramide functions as seen in diammonium di(nitramido) dinitroethylene (ADNDNE). The four compounds are predicted to have a low lipophilic nature. This favors migration to surface water or ground water but also indicates these compounds would not bioconcentrate into aquatic organisms or biomagnify within the food chain. Direct toxicity to aquatic organisms is also predicted to be very low.
Micron‐Scale Patterning of Biological Molecules (1995)

Site-specific attachment of biological molecules on gold and silicon dioxide surfaces has been achieved: The surface is modified with the receptor/ligand pair avidin D/photobiotin 1 and exposed to light through a mask in the presence of a protein solution; binding only occurs in the exposed areas. After unbound material is removed by washing, this process can be repeated several times to produce a two-dimensional pattern. One of the potential applications of this technique is the patterning of antibodies in a format that can be used in a multi-analyte immunosensor.
Using a packet switch for circuit-switched traffic: a queueing system with periodic input traffic (1989)

Consideration is given to the effects of time-multiplexed stream traffic on the performance of a store-and-forward packet switch. Substantially reducing the amount of buffering in the switch results in only a small probability that an existing circuit will be disrupted during the length of its connection. For example, with a circuit-switched frame of length 1000 and 100% loading, reducing the buffer size from 999 packets to 83 results in only a 10/sup -6/ circuit-disruption probability.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Spherical rotary piston machine as an artificial heart. (1992)

A positive displacement pump with six rotary pistons was proposed as an artificial heart. The pump's design was characterized by high symmetry and compactness. Thus, a spherical volume of 4 1/4 inch diameter sufficed for a pump delivering 10 L/min at 120 pulses/min with the pistons turning at 30 rpm. The pistons and four connecting gears were the only moving parts. The pump functions in two separate halves as left and right ventricles, with two of the six pistons each having inlet and outlet passages, and one of them replacing mitral and pulmonary valves with the other, tricuspid and aortic valves. The function of the intraventricular septum was provided by the other four pistons whose interiors also accommodated driving motors each capable of 0.4 Nm torque for a combined power of 5 watts. There were no stagnant regions in the pumping volume, and at all internal surfaces in contact with blood, there was periodic shear stress not exceeding approximately 300 Pa.
Redox and Fluorophore Functionalization of Water-Soluble, Tiopronin-Protected Gold Clusters (1999)

Place-exchange and amide-forming coupling reactions represent two facile and efficient routes to poly-functionalization of water-soluble nanoparticles. In this paper, place-exchange and amide-forming coupling reactions with water-soluble tiopronin-MPCs are described and their products characterized by 1H and 31P NMR, capillary electrophoresis, electrochemistry, and fluorescence spectroscopy. Place-exchange reactions of ligands with tiopronin-MPCs yield products with about half of the ligand exchange expected on the basis of solution stoichiometry and a nonselective exchange and were not noticeably affected by steric encumbrances. Tiopronin-MPCs to which viologens are coupled (avg 36/MPC) adsorb as monolayers on Au electrodes as shown in electrochemical quartz crystal microbalance experiments. Multilayer adsorption occurs on long experimental time scales. The strong adsorption of the viologen-functionalized clusters is ascribed to increased interaction and stability in the viologen reduction products. Capillary electrophoresis experiments with tiopronin-MPCs and viologen-functionalized tiopronin-MPCs reveal a number of separable core size/charge state combinations. Fluorescence measurements show ∼50% quenching of fluorescein upon attachment (avg 3.7/cluster) to tiopronin-MPCs, relative to the monomer under the same conditions. The results of this paper provide a pathway to explore poly-functionalized water-soluble nanoparticles in a variety of applications, including their use as biosensors.
Fgf-10 is required for both limb and lung development and exhibits striking functional similarity to Drosophila branchless (1998)

Fgf-10-deficient mice (Fgf-10(-/-)) were generated to determine the role(s) of Fgf-10 in vertebrate development. Limb bud initiation was abolished in Fgf-10(-/-) mice. Strikingly, Fgf-10(-/-) fetuses continued to develop until birth, despite the complete absence of both fore- and hindlimbs. Fgf-10 is necessary for apical ectodermal ridge (AER) formation and acts epistatically upstream of Fgf-8, the earliest known AER marker in mice. Fgf-10(-/-) mice exhibited perinatal lethality associated with complete absence of lungs. Although tracheal development was normal, main-stem bronchial formation, as well as all subsequent pulmonary branching morphogenesis, was completely disrupted. The pulmonary phenotype of Fgf-10(-/-) mice is strikingly similar to that of the Drosophila mutant branchless, an Fgf homolog.
A Diverse Family of Host-Defense Peptides (Piscidins) Exhibit Specialized Anti-Bacterial and Anti-Protozoal Activities in Fishes (2016)

Conventional antibiotics and other chemical-based drugs are currently one of the most common methods used to control disease-related mortality in animal agriculture. Use of the innate immune system to decrease disease related mortalities is a novel alternative to conventional drugs. One component of the innate immune system is the host-defense peptides, also known as antimicrobial peptides. Host-defense peptides are typically small, amphipathic, α-helical peptides with a broad-spectrum of action against viral, bacterial, fungal, and/or protozoal pathogens. Piscidins are host-defense peptides first discovered in the hybrid striped bass (white bass, Morone chrysops, x striped bass, M. saxatilis). In this paper we identify four new piscidin isoforms in the hybrid striped bass and describe their tissue distributions. We also determine the progenitor species of origin of each piscidin (orthology) and propose a revised nomenclature for this newly described piscidin family based on a three class system. The Class I piscidins (22 amino acids in length; striped bass and white bass piscidin 1 and piscidin 3) show broad-spectrum activity against bacteria and ciliated protozoans, while the Class III piscidins (55 amino acids in length; striped bass and white bass piscidin 6 and striped bass piscidin 7) primarily show anti-protozoal activity. The Class II piscidins (44-46 amino acids in length; striped bass and white bass piscidin 4 and white bass piscidin 5) have a level of activity against bacteria and protozoans intermediate to Classes I and III. Knowledge of piscidin function and activity may help in the future development of disease-resistant lines of striped bass and white bass that could be used to produce superior hybrids for aquaculture.
Deaths: Preliminary Data for 2006 (2008)

Objectives—This report presents preliminary U.S. data on deaths, death rates, life expectancy, leading causes of death, and infant mortality for the year 2006 by selected characteristics such as age, sex, race, and Hispanic origin. Methods—Data in this report are based on a large number of deaths comprising approximately 99 percent of the demographic file and 96 percent of the medical file for all deaths in the United States in 2006. The records are weighted to independent control counts for 2006. For certain causes of death such as unintentional injuries, homicides, suicides, and sudden infant death syndrome, preliminary and final data may differ because of the truncated nature of the preliminary file. Comparisons are made with 2005 final data. Results—The age-adjusted death rate for the United States decreased from 798.8 deaths per 100,000 population in 2005 to 776.4 deaths per 100,000 population in 2006. Age-adjusted death rates
Design of a nonviral vector for site‐selective, efficient integration into the human genome (2002)

Gene therapy in eukaryotes has met many obstacles. Research into the design of suitable nonviral vectors has been slow. To our knowledge, no nonviral vector has been proposed that allows for the possibility of highly efficient, site-selective integration into the genome of mammalian cells. On the basis of prior studies investigating the components necessary for transposon, retrovirus-like retrotransposon, and retroviral integration, we propose a nonviral system that would potentially allow for site-selective, efficient integration into the mammalian genome. Transposons have been developed that can transform a variety of cell lines. For example, the Sleeping Beauty transposon (SB) can transform a wide range of vertebrate cells from fish to human, and it mediates stable integration and long-term transgene expression in mice. However, the efficiency of transposition varies significantly among cell lines, suggesting the possible involvement of host factors in SB transposition. Here, we propose the use of a chimeric transposase (i.e., transposase-host DNA binding domain) to bypass the potential requirement of a host DNA-directing factor (or factors) for efficient, site-selective integration. We also discuss another potential method of docking the transposon-based vector adjacent to the host DNA, utilizing repetitive sequences for homologous recombination to promote efficient site-selective integration, as well as other site-selective nonviral approaches.
The ring laser gyro (1985)

This paper presents a review of both active and passive ring laser devices. The operating principles of the ring laser are developed and discussed, with special emphasis given to the problems associated with the achievement of greater sensitivity and stability. First-principle treatments of the nature of quantum noise in the ring laser gyro and various methods designed to avoid low-rotation-rate lock-in are presented. Descriptions of state-of-the-art devices and current and proposed applications (including a proposed test of metric theories of gravity using a passive cavity ring laser) are given.
Behavioral evidence of trigeminal neuropathic pain following chronic constriction injury to the rat's infraorbital nerve (1994)

Video recordings of free behavior and responses to mechanical facial stimulation were analyzed to assess whether chronic constriction injury (CCI) to the rat's infraorbital nerve (IoN) results in behavioral alterations indicative of neuropathic pain. A unilateral CCI was produced by placing loose chromic gut ligatures around the IoN. After CCI to the IoN, rats exhibited changes in both non-evoked and evoked behavior. Behavioral changes developed in two phases. Early after CCI (postoperative days 1–15), rats showed increased face-grooming activity with face-wash strokes directed to the injured nerve territory, while the responsiveness to stimulation of this area was decreased. Later after CCI (postoperative days 15–130), the prevalence of asymmetric face grooming was reduced but remained significantly increased compared to control rats. The early hyporesponsiveness was abruptly replaced by an extreme hyperresponsiveness: all stimulus intensities applied to the injured nerve territory evoked the “maximal” response (brisk head withdrawal, avoidance behavior plus directed face grooming). This response was never observed in control rats. Concurrently, IoN ligation rats showed a limited increase in the responsiveness to stimulation of the contralateral IoN territory, and around postoperative days 30–40 the responsiveness to stimulation of facial areas outside the IoN territories also increased. The hyperresponsiveness to stimulation of the ligated IoN territory slightly decreased from 60 d postoperative. Throughout the study, IoN ligation rats showed decreased exploratory behavior, displayed more freezing-like behavior, had a slower body weight gain, and a higher defecation rate, compared to control rats. The behavioral alterations observed after CCI to the IoN are indicative of severe sensory disturbances within the territory of the injured nerve: mechanical allodynia develops after a period of relative hypo- /anesthesia during which behavioral signs of recurrent spontaneous, aversive (possibly painful) sensations (paresthesias/dysesthesias) are maximal.
Persistence of Anaplasma ovis Infection and Conservation of the msp-2 and msp-3 Multigene Families within the Genus Anaplasma (1998)

Goats which have recovered from acute Anaplasma ovis infection remain seropositive, although infected erythrocytes cannot be detected by microscopic examination. Persistence of A. ovis 17 to 21 months following experimental infection was demonstrated by PCR detection of the msp-5 gene. Quantitative analysis of persistent rickettsemia over time showed that all levels were below the limit of microscopic detection and ranged from a low of 10(2) organisms/ml to peaks of 10(6) organisms/ml. Two patterns of persistent rickettsemia were observed: the first was characterized by cyclic fluctuations at 6- to 9-week intervals, similar to the pattern described for A. marginale-infected cattle, while in the second pattern, repetitive cycles did not occur and the rickettsemia levels were relatively constant. The msp-2 and msp-3 multigene families, which provide the genetic capacity for outer membrane protein antigenic variation during persistent A. marginale rickettsemia, were identified in the A. ovis genome by Southern blot analysis, and expression of an MSP-2 homologue was confirmed by using immunoblots.
Tyrosine phosphatase CD45 is required for T-cell antigen receptor and CD2-mediated activation of a protein tyrosine kinase and interleukin 2 production. (1991)

CD45, a hematopoietic cell-specific surface antigen, has recently been shown to be a protein tyrosine phosphatase. Expression of CD45 is essential for the T-cell antigen receptor to couple with the phosphatidylinositol second messenger pathway and for antigen-mediated proliferation of T lymphocytes. In this report we describe a CD45-deficient mutant of the human T-cell leukemia line Jurkat. CD45 expression is required for the activation of a T-cell receptor-associated tyrosine kinase as well as the phosphatidylinositol pathway. Additionally, stimulation of T lymphocytes by way of the accessory molecule CD2 requires the expression of CD45. The mutation in the CD45-deficient cell specifically impairs signal transduction by the T-cell receptor and CD2 because activation events by way of another accessory molecule, CD28, are unimpaired.
The imprinted gene and parent-of-origin effect database (2001)

The database of imprinted genes and parent-of-origin effects in animals (http://www.otago.ac.nz/IGC ) is a collation of genes and phenotypes for which parent-of-origin effects have been reported. The database currently includes over 220 entries, which describe over 40 imprinted genes in human, mouse and other animals. In addition a wide variety of other parent-of-origin effects, such as transmission of human disease phenotypes, transmission of QTLs, uniparental disomies and interspecies crosses are recorded. Data are accessed through a search engine and references are hyperlinked to PubMed.
Sustaining the graft-versus-tumor effect through posttransplant immunization with granulocyte-macrophage colony-stimulating factor (GM-CSF)–producing tumor vaccines (2000)

For many cancers, autologous bone marrow transplantation (BMT) achieves a minimal residual disease state, yet relapse rates remain high. Using a syngeneic murine bone marrow transplant model, we demonstrate that vaccination with irradiated granulocyte-macrophage colony-stimulating factor (GM-CSF)-producing autologous tumor cells is effective in the post-BMT period and actually results in a greater tumor-free survival than vaccination in the nontransplant setting. Employing T cells specific for a model tumor-antigen, we find that transplantation of the tumor-bearing host results in a massive expansion and activation of tumor-specific T cells in the early posttransplant period, but this response rapidly declines in association with tumor progression. Immunization with irradiated GM-CSF tumor cells during the period of immune reconstitution results in the sustained amplification and activation of this response that closely correlates with freedom from relapse. These results demonstrate the feasibility of integrating GM-CSF vaccines in the postautologous BMT setting and suggest mechanisms that may contribute to the observed efficacy of immunization during the critical period of immune reconstitution.
Quantification of chemotactic peptides (C5a anaphylatoxin and IL-8) in psoriatic lesional skin (1993)

<h3>• Background and Design.—</h3> Psoriatic scale extracts contain a unique chemotactic peptide fraction that is likely to be involved in the induction of rhythmic transepidermal leukocyte chemotaxis. Recent studies have identified the presence of two unrelated chemotactic peptides in this fraction, ie, C5a/ C5a des Arg and interleukin 8 (IL-8), and its related cytokines. To investigate their relative contribution to the transepidermal leukocyte migration as well as their interrelationship in psoriatic lesions, we have quantified concentrations of immunoreactive C5a/C5a des Arg and IL-8 in psoriatic lesional scale extracts and those from related sterile pustular dermatoses such as subcorneal pustular dermatosis and pustulosis palmaris et plantaris. <h3>Results.—</h3> The concentrations of C5a/C5a des Arg and IL-8 were more significantly increased in the horny-tissue extracts from lesional skin than in those from noninflammatory orthokeratotic skin (<i>P</i>&lt;.01). The increase of C5a/C5a des Arg concentration was specific to the lesional scale extracts, but showed a rather wide range of variation. By contrast, IL-8 concentration, although consistently increased in the lesional scale extracts, was also moderately increased even in noninflammatory scale extracts prepared from ichthyosis vulgaris. The elevation of IL-8 levels in psoriatic lesions was also confirmed by measuring their levels in cutaneous tissue fluid samples collected from suction blisters. However, unexpectedly, some control samples obtained from normal skin also showed a moderate increase in the IL-8 level. Neutrophil chemotactic activity correlated significantly only with the levels of C5a/C5a des Arg in the scales (<i>P</i>&lt;.05). No such significant correlation was found between chemotactic activity and IL-8 or between C5a/C5a des Arg and IL-8. <h3>Conclusion.—</h3> Based on these results, we speculate that, although IL-8 may exert a synergistic effect with C5a/C5a des Arg in the induction of transepidermal leukocyte chemotaxis, it constitutes a proinflammatory cytokine that is involved in the production of the persistent inflammatory changes characterized by a T-lymphocyte infiltration. In contrast, C5a/C5a des Arg seems to be generated only in the inflammatory lesional skin under specific circumstances that preferentially favor complement activation and also seems to play a major role in the induction of cyclic transepidermal leukocyte chemotaxis from "squirting papillae." (<i>Arch Dermatol.</i>1993;129:74-80)
Mepyramine, a Histamine H1 Receptor Inverse Agonist, Binds Preferentially to a G Protein-coupled Form of the Receptor and Sequesters G Protein (2004)

Accurate characterization of the molecular mechanisms of the action of ligands is an extremely important issue for their appropriate research, pharmacological, and therapeutic uses. In view of this fact, the aim of the present work was to investigate the mechanisms involved in the actions of mepyramine at the guinea pig H(1) receptor stably expressed in Chinese hamster ovary cells. We found that mepyramine is able to decrease the basal constitutive activity of the guinea pig H(1) receptor, to bind with high affinity to a G(q/11) protein-coupled form of the receptor and to promote a G protein-coupled inactive state of the H(1) receptor that interferes with the G(q/11)-mediated signaling of the endogenously expressed ATP receptor, as predicted by the Cubic Ternary Complex Model of receptor occupancy. The effect of mepyramine on ATP-induced signaling was specifically neutralized by Galpha(11) overexpression, indicating that mepyramine is able to reduce G protein availability for other non-related receptors associated with the same signaling pathway. Finally, we found a loss of mepyramine efficacy in decreasing basal levels of intracellular calcium at high Galpha(11) expression levels, which can be theoretically explained in terms of high H(1) receptor constitutive activity. The whole of the present work sheds new light on H(1) receptor pharmacology and the mechanisms H(1) receptor inverse agonists could use to exert their observed negative efficacy.
A Polyhedral Host Constructed Using a Linear Template (2002)

A linear template is used to direct the synthesis of a "head-to-head" tetrapyridine in the solid state, which, in a second step, assembles with a transition-metal-ion to form a polyhedral metal-organic host with a structure that conforms to a trigonal antiprism. The application of the linear template is reminiscent of the synthetic strategy of nature.