gebhart/patrepeval huggingface dataset:
Tissue Specificity of E- and P-Selectin Ligands in Th1-Mediated Chronic Inflammation (1999)

The demonstrated role of E- and P-selectin ligands in the recruitment of Th1 cells raises the question of tissue specificity determination by pathogenic T cells. We took advantage of the fact that chronic Th1-mediated inflammation in the scid/scid CD4+CD45RBhigh T cell transfer model can occur at multiple tissue sites, resembling inflammatory bowel disease in the colon and psoriasis in the skin. We show that the majority of infiltrating effector T cells from psoriatic skin expresses high levels of functional P-selectin ligand (87 +/- 3%), detected by P-selectin-Ig (PIg), while a significantly smaller subset of T cells from colitic lesions expresses this ligand (24 +/- 2%). Similarly, E-selectin ligand is preferentially expressed on CD4+ T cells infiltrating the skin (24 +/- 2%), but only on very few CD4+ T cells infiltrating the colon (CIT; 1.3 +/- 0.8%). In contrast, CD4+ T cells infiltrating the skin express alpha4beta7 at a significantly lower level than CIT (mean fluorescence intensity, 28 vs 61, respectively), although, interestingly, alphaEbeta7 was expressed at high levels on both populations. Analysis of the disease-inducing potential of PIg+ and PIg- CD4+ CIT cells revealed that both populations not only express similar levels of the gut-homing molecule alpha4beta7 (mean fluorescence intensity, 50 vs 56, respectively), but do not differ in their capacity to express IFN-gamma. Furthermore, CIT depleted of cells expressing functional P-selectin ligand were able to induce colitis upon transfer, suggesting that induction of colitis in this model may be independent of E- and P-selectin. These results indicate that adhesion molecule expression and the homing pattern of inflammatory T cells are regulated by the local environment independently of their inflammatory capacity.
Interactions between Growth Factors and Integrins: Latent Forms of Transforming Growth Factor-β Are Ligands for the Integrin αvβ1 (1998)

The multipotential cytokine transforming growth factor-beta (TGF-beta) is secreted in a latent form. Latency results from the noncovalent association of TGF-beta with its processed propeptide dimer, called the latency-associated peptide (LAP); the complex of the two proteins is termed the small latent complex. Disulfide bonding between LAP and latent TGF-beta-binding protein (LTBP) produces the most common form of latent TGF-beta, the large latent complex. The extracellular matrix (ECM) modulates the activity of TGF-beta. LTBP and the LAP propeptides of TGF-beta (isoforms 1 and 3), like many ECM proteins, contain the common integrin-binding sequence RGD. To increase our understanding of latent TGF-beta function in the ECM, we determined whether latent TGF-beta1 interacts with integrins. A549 cells adhered and spread on plastic coated with LAP, small latent complex, and large latent complex but not on LTBP-coated plastic. Adhesion was blocked by an RGD peptide, and cells were unable to attach to a mutant form of recombinant LAP lacking the RGD sequence. Adhesion was also blocked by mAbs to integrin subunits alphav and beta1. We purified LAP-binding integrins from extracts of A549 cells using LAP bound to Sepharose. alphavbeta1 eluted with EDTA. After purification in the presence of Mn2+, a small amount of alphavbeta5 was also detected. A549 cells migrated equally on fibronectin- and LAP-coated surfaces; migration on LAP was alphavbeta1 dependent. These results establish alphavbeta1 as a LAP-beta1 receptor. Interactions between latent TGF-beta and alphavbeta1 may localize latent TGF-beta to the surface of specific cells and may allow the TGF-beta1 gene product to initiate signals by both TGF-beta receptor and integrin pathways.
Lipid-insertion enables targeting functionalization of erythrocyte membrane-cloaked nanoparticles (2013)

RBC membrane-cloaked polymeric nanoparticles represent an emerging nanocarrier platform with extended circulation in vivo. A lipid-insertion method is employed to functionalize these nanoparticles without the need for direct chemical conjugation. Insertion of both folate and the nucleolin-targeting aptamer AS1411 shows receptor-specific targeting against model cancer cell lines.
Broadband triangular microstrip antenna with U-shapedslot (1997)

The broadband design of a triangular microstrip antenna with a U-shaped slot is demonstrated. With a foam substrate of thickness ~0.08λ0, a slotted triangular microstrip antenna with an operating bandwidth of ~18% is obtained, which is ~ 1.8 times that of a corresponding simple triangular patch antenna. Details of the antenna design and experimental results are presented.
Applications of high‐speed confocal imaging techniques in operative dentistry (1994)

All confocal scanning optical microscopes are suitable for making high-resolution images of many structures in teeth under near normal conditions. If the microscope can operate at high speed, then the number of applications widens considerably. The high-frame speed of the tandem scanning reflected light microscope (TSM) enables real-time examination of teeth in vivo, especially when the microscope is configured with a stabilising objective, featuring internal focusing elements. Experimental procedures examined microscopically on extracted teeth can include the cutting of the hard tissues, observation of fluid flow in dentine, the application of adhesives, and the fracturing of adhesive interfaces under load. Undertaking experiments where time is an important function has improved our knowledge of many of the materials/substrate interactions involved in dental operative procedures. Storing images on media other than video tape can be expensive, but reductions in the cost of computer memory is making digitisation and storage of images in real-time more widely available.
Synthesis and Anticoagulant Activity of Sulfated Glucoside-Bearing Polymer (1996)

Poly(glucosyloxyethyl methacrylate) sulfate [poly(GEMA) sulfate], which contains sulfated glucoside residues, was prepared by the reaction with N,N-dimethylformamide (DMF)/sulfur trioxide (SO3) complex. The degree of sulfation was easily controlled by changing the amount of DMF/SO3 complex added and reaction time. The total human blood clotting time in the presence of poly(GEMA) sulfate was prolonged by increasing the dose or the degree of sulfation of the polymer. The anticoagulant activity of poly(GEMA) sulfate was also compared with that of dextran sulfate, poly(styrenesulfonic acid), poly(vinylsulfuric acid), and heparin. The result suggests that sulfated saccharide residues are essential for endowing anticoagulant activity to synthetic polymer.
Proliferation and differentiation potential of rat forebrain oligodendroglial progenitors both in vitro and in vivo (1991)

We have followed the development of the O-2A progenitor cell from the neonatal rat forebrain, both in dissociated cell culture and in cryostat sections, using immunocytochemical techniques employing a panel of antibodies that recognise the cells at different stages of their development. This included the monoclonal antibody LB1, which binds to the surface ganglioside GD3 expressed on O-2A progenitor cells. In secondary cultures enriched for O-2A progenitors maintained in a serum-free chemically defined medium, a large proportion of the cells are primed to differentiate into oligodendroglia and go on to express the oligodendroglial specific surface glycolipid galactocerebroside (GC) and then the myelin proteins CNP and MBP. However, a significant proportion of immature bipolar GD3+ cells remained after 6 days in secondary culture. It appears that not all the O-2A progenitors in our cultures differentiate immediately and some cells remain in an undifferentiated state and divide to replenish progenitor numbers. We have also identified in our cultures a small apolar GD3- cell, which when isolated differentiated into a GD3+ bipolar O-2A progenitor cell. We have termed this cell type a preprogenitor. The differentiation of this cell type into O-2A progenitors may be the source of the immature GD3+ cells present at the later stages of our secondary cultures. The proliferative profile of the cultures was studied using 5'bromo-2-deoxyuridine (BrdU) incorporation as an index of mitosis. Only the immature, bipolar O-2A progenitors were seen to divide at any time in serum-free culture. Neither the more mature multipolar O-2A cells nor the oligodendroglia were seen to divide. The developmental profile of the O-2A cells in the rat forebrain in vivo showed a largely similar progression to that in culture, with a time lag of at least 6 days between GD3 expression and the onset of myelination. BrdU incorporation studies in vivo also showed that the GD3+ progenitor cell is mitotic whereas the GC(+)-expressing oligodendroglia is not. We have shown that there are several significant alterations in the timing of antigen expression in both O-2A progenitors and oligodendroglia in vitro compared to that seen in vivo.
Role of T cells in the B-cell response: glutaraldehyde-fixed T-helper hybridoma cells synergize with the lymphokine IL-4 to induce B-cell activation and proliferation. (1991)

Antigen-unselected helper T-cell hybridomas (Th) which activate normal resting B cells to RNA synthesis and proliferation in the presence of concanavalin A (Con A) have been developed. The response is completely Th cell dependent, and not restricted by the haplotype of the B-cell major histocompatibility complex (MHC). Culture supernatants from the Con A-stimulated Th hybridomas contain interleukin-4 (IL-4) and IL-2, but undetectable level of IL-5. The supernatant alone, however, does not induce B-cell activation or proliferation. Although the Con A-mediated Th cell-dependent B-cell response occurs in an MHC-unrestricted manner, the response of resting B cells can be blocked by monoclonal Ia antibody specific for the surface class II molecules of the responding B cell. The response is also blocked by monoclonal antibody to L3T4. Significant activation and proliferation of resting B cells can also be triggered by glutaraldehyde-fixed Th hybridomas and Con A when exogenous IL-4 is added. The stimulation with fixed Th hybridomas plus IL-4 can be inhibited by monoclonal anti-L3T4 or anti-Ia. These results suggest that maximal B-cell activation requires a direct helper T cell-B cell interaction which depends on availability of Ia on the B cell and L3T4 on the T cell, even when Con A overcomes the requirement for MHC-restricted T-cell recognition. We suggest that this signal, in conjunction with T-cell produced lymphokine IL-4, is responsible for the activation and subsequent proliferation of the B cells which occurs following interaction with T cells.
Direct Growth of Mono‐ and Multilayer Nanostructured Porous Films on Curved Surfaces and Their Application as Gas Sensors (2005)

Polystyrene sphere colloidal monolayers can be stripped off in some precursor solutions. A simple and flexible method is presented to directly synthesize nanostructured ordered porous films on any desired substrate with flat or even curved surfaces and then construct nanostructured porous gas sensors on commercially supplied ceramic tubes (see Figure).
Anatomical Abnormalities in the Brains of Monozygotic Twins Discordant for Schizophrenia (1990)

Recent neuroradiologic and neuropathological studies indicate that at least some patients with schizophrenia have slightly enlarged cerebral ventricles and subtle anatomical abnormalities in the region of the anterior hippocampus. Using magnetic resonance imaging (MRI), we studied 15 sets of monozygotic twins who were discordant for schizophrenia (age range, 25 to 44 years; 8 male and 7 female pairs). For each pair of twins, T1-weighted contiguous coronal sections (5 mm thick) were compared blindly, and quantitative measurements of brain structures were made with a computerized image-analysis system. In 12 of the 15 discordant pairs, the twin with schizophrenia was identified by visual inspection of cerebrospinal fluid spaces. In two pairs no difference could be discerned visually, and in one the twin with schizophrenia was misidentified. Quantitative analysis of sections through the level of the pes hippocampi showed the hippocampus to be smaller on the left in 14 of the 15 affected twins, as compared with their normal twins, and smaller on the right in 13 affected twins (both P less than 0.001). In the twins with schizophrenia, as compared with their normal twins, the lateral ventricles were larger on the left in 14 (P less than 0.003) and on the right in 13 (P less than 0.001). The third ventricle also was larger in 13 of the twins with schizophrenia (P less than 0.001). None of these differences were found in seven sets of monozygotic twins without schizophrenia who were studied similarly as controls. We conclude that subtle abnormalities of cerebral anatomy (namely, small anterior hippocampi and enlarged lateral and third ventricles) are consistent neuropathologic features of schizophrenia and that their cause is at least in part not genetic. Further study is required to determine whether these changes are primary or secondary to the disease.
Controlled release of BMP‐2 using a heparin‐conjugated carrier system reduces in vivo adipose tissue formation (2014)

There is growing concern about unwanted effects associated with the clinical use of recombinant human bone morphogenetic protein-2 (rhBMP-2) at high concentrations, including cyst-like bone formation and excessive fatty marrow formation. We, therefore, evaluated the induction of mineralized/adipose tissue formation and the bone-healing pattern associated with the controlled release of E. coli-derived rhBMP-2 (ErhBMP-2) by a heparin-conjugated fibrin (HCF) system using ectopic and orthotopic in vivo models, respectively. In the ectopic transplantation model, mineralized tissue formed at the most superficial layer of the transplanted area and on the surfaces of grafted materials, and most of the interstitial space within the transplanted area was filled with excessive adipose tissue specifically at sites that received ErhBMP-2. However, sites that received ErhBMP-2 and HCF showed significantly increased mineralized tissue formation and decreased adipose tissue formation compared to the normal fibrin system with ErhBMP-2. In the orthotopic (calvarial defect) model, controlled release of ErhBMP-2 induced by HCF significantly reduced adipose tissue formation within the defect area compared to the clinically approved absorbable collagen sponge. From these results, it can be concluded that the use of a HCF system loaded with ErhBMP-2 may reduce adipose tissue formation and enhance mineralized tissue formation.
Attenuation of cocaine-induced locomotor activity by butyrylcholinesterase. (1998)

A primary enzyme for the metabolism of cocaine is butyrylcholinesterase (BChE). To determine whether the systemic administration of BChE can increase the metabolism of cocaine sufficiently to alter a behavioral effect, rats were tested in a locomotor activity chamber after receiving 17 mg of cocaine per kg intraperitoneally. In rats pretreated intravenously with 5,000 IU of horse serum-derived BChE, the locomotor activity effect was significantly attenuated. BChE pretreatment increased plasma BChE levels approximately 400-fold. When added to rat plasma, this amount of BChE reduced the cocaine half-life from over 5 hr to less than 5 min. BChE altered the cocaine metabolic pattern such that the relatively nontoxic metabolite ecgonine methyl ester was produced, rather than benzoylecgonine. These results suggest that systemic administration of BChE can increase the metabolism of cocaine sufficiently to alter a behavioral effect of cocaine and thus should be investigated as a potential treatment for cocaine abuse.
Psychological Reactions and Sexual Life after Hysterectomy with and without Oophorectomy (1992)

A total of 678 women were interviewed about psychological reactions and sexual adjustment after abdominal hysterectomy with and without simultaneous oophorectomy. The response rate was high and complete questionnaires were returned by 86% of the women. A more positive attitude towards the operation was found in the group of women where the ovaries had been preserved. Women who had undergone oophorectomy experienced a deteriorated sexual life compared to women with preserved ovaries. This was observed regardless of age and in different parameters such as ‘coital frequency’ and ‘experience of intercourse’. The negative effects on sexual life as a result of hysterectomy with simultaneous oophorectomy are important to bear in mind and should be discussed with the woman prior to surgery.
Robust perceptual image hashing via matrix invariants (2005)

In this paper we suggest viewing images (as well as attacks on them) as a sequence of linear operators and propose novel hashing algorithms employing transforms that are based on matrix invariants. To derive this sequence, we simply cover a two dimensional representation of an image by a sequence of (possibly overlapping) rectangles R/sub i/ whose sizes and locations are chosen randomly/sup 1/ from a suitable distribution. The restriction of the image (representation) to each R/sub i/ gives rise to a matrix A/sub i/. The fact that A/sub i/'s will overlap and are random, makes the sequence (respectively) a redundant and non-standard representation of images, but is crucial for our purposes. Our algorithms first construct a secondary image, derived from input image by pseudo-randomly extracting features that approximately capture semi-global geometric characteristics. From the secondary image (which does not perceptually resemble the input), we further extract the final features which can be used as a hash value (and can be further suitably quantized). In this paper, we use spectral matrix invariants as embodied by singular value decomposition. Surprisingly, formation of the secondary image turns out be quite important since it not only introduces further robustness (i.e., resistance against standard signal processing transformations), but also enhances the security properties (i.e. resistance against intentional attacks). Indeed, our experiments reveal that our hashing algorithms extract most of the geometric information from the images and hence are robust to severe perturbations (e.g. up to %50 cropping by area with 20 degree rotations) on images while avoiding misclassification. Our methods are general enough to yield a watermark embedding scheme, which will be studied in another paper.
Selective laser melting of Inconel 625 using pulse shaping (2010)

Purpose The purpose of this paper is to investigate the selective laser melting (SLM) of Inconel 625 using pulse shape control to vary the energy distribution within a single laser pulse. It aims to discuss the effectiveness of pulse shaping, including potential benefits for use within SLM. Design/methodology/approach Laser parameters were varied in order to identify optimal parameters that produced thin wall parts with a low surface roughness without the use of pulse shape control. Pulse shape control was then employed to provide gradual heating or a prolonged cooling effect with a variety of peak power/pulse energy combinations. Properties of pulse shaped and nonpulse shaped parts were compared, with particular attention focused on part surface roughness and width. Findings High peak powers tended to reduce top surface roughness and reduce side roughness as recoil pressures flatten out the melt pool and inhibit melt pool instabilities from developing. Ramp up energy distribution can reduce the maximum peak power required to melt material and reduce material spatter generation during processing due to a localized preheating effect. Ramp down energy distribution prolonged melt pool solidification allowing more time for molten material to redistribute, subsequently reducing the top surface roughness of parts. However, larger melt pools and longer solidification times increased the side roughness of parts due to a possible lateral expulsion of material from the melt pool. Originality/value This paper is the first of its kind to employ laser pulse shape control during SLM to process material from powder bed. It is a useful aid in unveiling relationships between laser energy distribution and the formation of parts.
Combined Antibacterial and Anti-Inflammatory Activity of a Cationic Disubstituted Dexamethasone-Spermine Conjugate (2010)

The rising number of antibiotic-resistant bacterial strains represents an emerging health problem that has motivated efforts to develop new antibacterial agents. Endogenous cationic antibacterial peptides (CAPs) that are produced in tissues exposed to the external environment are one model for the design of novel antibacterial compounds. Here, we report evidence that disubstituted dexamethasone-spermine (D2S), a cationic corticosteroid derivative initially identified as a by-product of synthesis of dexamethasone-spermine (DS) for the purpose of improving cellular gene delivery, functions as an antibacterial peptide-mimicking molecule. This moiety exhibits bacterial killing activity against clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa present in cystic fibrosis (CF) sputa, and Pseudomonas aeruginosa biofilm. Although compromised in the presence of plasma, D2S antibacterial activity resists the proteolytic activity of pepsin and is maintained in ascites, cerebrospinal fluid, saliva, and bronchoalveolar lavage (BAL) fluid. D2S also enhances S. aureus susceptibility to antibiotics, such as amoxicillin (AMC), tetracycline (T), and amikacin (AN). Inhibition of interleukin-6 (IL-6) and IL-8 release from lipopolysaccharide (LPS)- or lipoteichoic acid (LTA)-treated neutrophils in the presence of D2S suggests that this molecule might also prevent systemic inflammation caused by bacterial wall products. D2S-mediated translocation of green fluorescent protein (GFP)-labeled glucocorticoid receptor (GR) in bovine aorta endothelial cells (BAECs) suggests that some of its anti-inflammatory activities involve engagement of glucocorticoid receptors. The combined antibacterial and anti-inflammatory activities of D2S suggest its potential as an alternative to natural CAPs in the prevention and treatment of some bacterial infections.
Effect of Botulinum Toxin A Chemodenervation in Sensory Strabismus (2001)

Botulinum toxin type A is likely to prevent muscle contracture and affect muscle and neuronal tissues. This study on the effects of sensory strabismus with botulinum toxin type A injection suggests it has the potential to replace surgery or be used as an adjuvant therapy.