gebhart/patrepeval huggingface dataset:
Human immunodeficiency virus type 1 infection despite prior immunization with a recombinant envelope vaccine regimen. (1996)

With efforts underway to develop a preventive human immunodeficiency virus type 1 (HIV-1) vaccine, it remains unclear which immune responses are sufficient to protect against infection and whether prior HIV-1 immunity can alter the subsequent course of HIV-1 infection. We investigated these issues in the context of a volunteer who received six HIV-1LAI envelope immunizations and 10 weeks thereafter acquired HIV-1 infection through a high-risk sexual exposure. In contrast to nonvaccinated acutely infected individuals, anamnestic HIV-1-specific B- and T-cell responses appeared within 3 weeks in this individual, and neutralizing antibody preceded CD8+ cytotoxic responses. Despite an asymptomatic course and an initial low level of detectable infectious virus, a progressive CD4+ cell decline and dysfunction occurred within 2 years. Although vaccination elicited immunity to HIV-1 envelope, which was recalled upon HIV-1 exposure, it was insufficient to prevent infection and subsequent immunodeficiency.
Cloning, sequencing, and expression of cDNA for human beta-glucuronidase. (1987)

We report here the cDNA sequence for human placental beta-glucuronidase (beta-D-glucuronoside glucuronosohydrolase, EC 3.2.1.31) and demonstrate expression of the human enzyme in transfected COS cells. We also sequenced a partial cDNA clone from human fibroblasts that contained a 153-base-pair deletion within the coding sequence and found a second type of cDNA clone from placenta that contained the same deletion. Nuclease S1 mapping studies demonstrated two types of mRNAs in human placenta that corresponded to the two types of cDNA clones isolated. The NH2-terminal amino acid sequence determined for human spleen beta-glucuronidase agreed with that inferred from the DNA sequence of the two placental clones, beginning at amino acid 23, suggesting a cleaved signal sequence of 22 amino acids. When transfected into COS cells, plasmids containing either placental clone expressed an immunoprecipitable protein that contained N-linked oligosaccharides as evidenced by sensitivity to endoglycosidase F. However, only transfection with the clone containing the 153-base-pair segment led to expression of human beta-glucuronidase activity. These studies provide the sequence for the full-length cDNA for human beta-glucuronidase, demonstrate the existence of two populations of mRNA for beta-glucuronidase in human placenta, only one of which specifies a catalytically active enzyme, and illustrate the importance of expression studies in verifying that a cDNA is functionally full-length.
"SKIN PENETRATION." (1941)

Journal Article “SKIN PENETRATION.” Get access RALPH G. HARRY, F.I.C. RALPH G. HARRY, F.I.C. Certificate of the Institute of Chemistry in Foods, and Drugs, Pharmacology and Therapeutics, Associate of the Royal Photographic Society Search for other works by this author on: Oxford Academic Google Scholar British Journal of Dermatology and Syphilis, Volume 53, Issue 3, 1 March 1941, Pages 65–82, https://doi.org/10.1111/j.1365-2133.1941.tb10505.x Published: 01 March 1941
Inhibition of Apoptosis by Z-VAD-fmk in SMN-depleted S2 Cells (2003)

Spinal muscular atrophy is an autosomal recessive motor neuron degenerative disorder, caused by the loss of telomeric copy of the survival motor neuron gene (SMN1). To better understand how motor neurons are targeted in Spinal muscular atrophy patients, it is important to study the role of SMN protein in cell death. In this report, we employed RNA interference (RNAi) to study the loss-of-function of SMN in Drosophila S2 cells. A 601-base pair double-stranded RNA (dsRNA) of Drosophila SMN (dSMN) was used for silencing the dSMN. Our data indicate that dSMN RNAi resulted in more than 90% reduction of both RNA and protein. Further analysis of S2 cells by cell death ELISA and flow cytometry assays revealed that reduction of dSMN expression significantly increased apoptosis. The cell death mediated by SMN depletion is caspase-dependent and specifically due to the activation of the endogenous caspases, DRONC and DRICE. Significantly, the effect of dSMN RNAi was reversed by a peptide caspase inhibitor, Z-VAD-fmk. These results suggest that dSMN is involved in signal pathways of apoptotic cell death in Drosophila. Hence, the model system of reduced SMN expression by RNAi in Drosophila could be exploited for identification of therapeutic targets.
Strong expression of foreign genes following direct injection into fish muscle (1991)

We report here for the first time direct injection of genes into fish muscle in vivo. Plasmids used contain either SV40 early promoter, rabbit beta-cardiac myosin heavy chain promoter, human MxA promoter or an artificial promoter, fused to a chloramphenicol acetyltransferase (CAT) or beta-galactosidase reporter gene. CAT assays revealed that most gene constructs were highly expressed. Histochemical analysis showed that beta-galactosidase was strongly expressed at the site of injection within muscle fibres. This method provides an excellent system for testing expression of gene constructs, including those of mammalian origin, in fish muscle in vivo and has the potential for fish vaccination.
Interleukin-12 (1994)

Interleukin-12 (IL-12) is a newly characterized cytokine that has a unique heterodimeric structure. It was initially cloned from B lymphoblastoid cell lines, but the majority of IL-12 is produced by macrophages/monocytes following appropriate stimulation. IL-12 can (1) enhance the cytolytic activity of a number of effector cells including T cells, natural killer (NK) cells, lymphokine activated killer (LAK) cells, and macrophages, (2) increase proliferation of activated NK and T cells, (3) induce production of cytokines, such as interferon gamma, (4) stimulate the induction of TH1 cells, (5) upregulate a number of cell surface molecules, (6) inhibit IgE secretion, and (7) act as a synergistic factor for hematopoietic stem cells. Based on these potent immunomodulatory activities, IL-12 has been evaluated in several disease models for parasitic infections and malignancies. Marked activity of IL-12 against both Leishmania and Toxoplasma has been reported. Likewise, antimetastatic and antitumor activity, including tumor regression, has been observed against a number of murine malignancies treated with IL-12 using doses that result in little toxicity. The results suggest that IL-12 may be a useful cytokine for the treatment of a number of diseases.
Templating of Mesoporous Molecular Sieves by Nonionic Polyethylene Oxide Surfactants (1995)

Mesoporous silica molecular sieves have been prepared by the hydrolysis of tetraethylorthosilicate in the presence of low-cost, nontoxic, and biodegradable polyethylene oxide (PEO) surfactants, which act as the structure-directing (templating) agents. This nonionic, surfactant-neutral, inorganic-precursor templating pathway to mesostructures uses hydrogen bonding interactions between the hydrophilic surfaces of flexible rod- or worm-like micelles and Si(OC(2)H(5))(4-x)(OH)(x) hydrolysis products to assemble an inorganic oxide framework. Disordered channel structures with uniform diameters ranging from 2.0 to 5.8 nanometers have been obtained by varying the size and structure of the surfactant molecules. Metal-substituted silica and pure alumina mesostructures have also been prepared by the hydrolysis of the corresponding alkoxides in the presence of PEO surfactants. These results suggest that nonionic templating may provide a general pathway for the preparation of mesoporous oxides.
Smokey: automatic recognition of hostile messages (1997)

Abusive messages (flames) can be both a source of frustration and a waste of time for Internet users. This paper describes some approaches to flame recognition, including a prototype system, Smokey. Smokey builds a 47-element feature vector based on the syntax and semantics of each sentence, combining the vectors for the sentences within each message. A training set of 720 messages was used by Quinlan&amp;apos;s C4.5 decision-tree generator to determine featurebased rules that were able to correctly categorize 64% of the flames and 98% of the non-flames in a separate test set of 460 messages. Additional techniques for greater accuracy and user customization are also discussed. Introduction Flames are one of the current hazards of on-line communication. While some people enjoy exchanging flames, most users consider these abusive and insulting messages to be a nuisance or even upsetting. I describe Smokey, a prototype system to automatically recognize email flames. Smokey combines natural-langu...
Circulating Adiponectin Levels Are Reduced in Nonobese but Insulin-Resistant First-Degree Relatives of Type 2 Diabetic Patients (2003)

Adiponectin, one of the most abundant gene transcript proteins in human fat cells, has been shown to improve insulin action and is also suggested to exert antiatherogenic effects. We measured circulating adiponectin levels and risk factors for atherosclerosis in 45 healthy first-degree relatives of type 2 diabetic subjects (FDR) as well as 40 healthy control subjects (CON) without a known family history of diabetes. Insulin sensitivity (S(i)) was studied with the minimal model, and measurements of adiponectin, metabolic variables, inflammatory markers, and endothelial injury markers, as well as lipoprotein concentrations, were performed. FDR were insulin resistant (3.3 +/- 2.4 vs. 4.5 +/- 2.6 x 10(-4) x min(-1) per microU/ml [mean +/- SD], P < 0.01), and their circulating plasma adiponectin levels (6.6 +/- 1.8 vs. 8.1 +/- 3.0 microg/ml, P < 0.03) were decreased. After adjustments for age in FDR, adiponectin levels were negatively correlated with fasting proinsulin (r -0.64, P < 0.001), plasminogen activator inhibitor (PAI)-1 activity (r -0.56, P < 0.001), fasting insulin (r -0.55, P < 0.001), and acute insulin response (r -0.40, P < 0.05); they were positively related to HDL cholesterol (r 0.48, P < 0.01) and S(i) (r 0.41, P < 0.01). Furthermore, when adjusted for age, waist, and S(i), adiponectin was associated with HDL cholesterol and proinsulin, which explained 51% of the variation in adiponectin in multiple regression analyses in that group. In conclusion, circulating plasma adiponectin levels were decreased in nonobese but insulin-resistant FDR and, in addition, related to several facets of the insulin resistance syndrome (IRS). Thus, hypoadiponectinemia may be an important component of the association between cardiovascular disease and IRS.
Xenopus nonmuscle myosin heavy chain isoforms have different subcellular localizations and enzymatic activities. (1996)

There are two isoforms of the vertebrate nonmuscle myosin heavy chain, MHC-A and MHC-B, that are encoded by two separate genes. We compared the enzymatic activities as well as the subcellular localizations of these isoforms in Xenopus cells. MHC-A and MHC-B were purified from cells by immunoprecipitation with isoform-specific peptide antibodies followed by elution with their cognate peptides. Using an in vitro motility assay, we found that the velocity of movement of actin filaments by MHC-A was 3.3-fold faster than that by MHC-B. Likewise, the Vmax of the actin-activated Mg(2+)-ATPase activity of MHC-A was 2.6-fold greater than that of MHC-B. Immunofluorescence microscopy demonstrated distinct localizations for MHC-A and MHC-B. In interphase cells, MHC-B was present in the cell cortex and diffusely arranged in the cytoplasm. In highly polarized, rapidly migrating interphase cells, the lamellipodium was dramatically enriched for MHC-B suggesting a possible involvement of MHC-B based contractions in leading edge extension and/or retraction. In contrast, MHC-A was absent from the cell periphery and was arranged in a fibrillar staining pattern in the cytoplasm. The two myosin heavy chain isoforms also had distinct localizations throughout mitosis. During prophase, the MHC-B redistributed to the nuclear membrane, and then resumed its interphase localization by metaphase. MHC-A, while diffuse within the cytoplasm at all stages of mitosis, also localized to the mitotic spindle in two different cultured cell lines as well as in Xenopus blastomeres. During telophase both isoforms colocalized to the contractile ring. The different subcellular localizations of MHC-A and MHC-B, together with the data demonstrating that these myosins have markedly different enzymatic activities, strongly suggests that they have different functions.
Chemical Sensors Based on Fiber Optics (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical Sensors Based on Fiber OpticsW. Rudolf SeitzCite this: Anal. Chem. 1984, 56, 1, 16A–34APublication Date (Print):January 1, 1984Publication History Published online14 December 2011Published inissue 1 January 1984https://pubs.acs.org/doi/10.1021/ac00265a711https://doi.org/10.1021/ac00265a711research-articleACS PublicationsRequest reuse permissionsArticle Views900Altmetric-Citations230LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Serum interleukin-15 levels are not elevated in patients with stage I and II mycosis fungoides (2000)

Abstract is missing
Motion2fusion (2017)

We present Motion2Fusion, a state-of-the-art 360 performance capture system that enables *real-time* reconstruction of arbitrary non-rigid scenes. We provide three major contributions over prior work: 1) a new non-rigid fusion pipeline allowing for far more faithful reconstruction of high frequency geometric details, avoiding the over-smoothing and visual artifacts observed previously. 2) a high speed pipeline coupled with a machine learning technique for 3D correspondence field estimation reducing tracking errors and artifacts that are attributed to fast motions. 3) a backward and forward non-rigid alignment strategy that more robustly deals with topology changes but is still free from scene priors. Our novel performance capture system demonstrates real-time results nearing 3x speed-up from previous state-of-the-art work on the exact same GPU hardware. Extensive quantitative and qualitative comparisons show more precise geometric and texturing results with less artifacts due to fast motions or topology changes than prior art.
Strongly bound citrate stabilizes the apatite nanocrystals in bone (2010)

Nanocrystals of apatitic calcium phosphate impart the organic-inorganic nanocomposite in bone with favorable mechanical properties. So far, the factors preventing crystal growth beyond the favorable thickness of ca. 3 nm have not been identified. Here we show that the apatite surfaces are studded with strongly bound citrate molecules, whose signals have been identified unambiguously by multinuclear magnetic resonance (NMR) analysis. NMR reveals that bound citrate accounts for 5.5 wt% of the organic matter in bone and covers apatite at a density of about 1 molecule per (2 nm)(2), with its three carboxylate groups at distances of 0.3 to 0.45 nm from the apatite surface. Bound citrate is highly conserved, being found in fish, avian, and mammalian bone, which indicates its critical role in interfering with crystal thickening and stabilizing the apatite nanocrystals in bone.
Pax-5 (BSAP) recruits Ets proto-oncogene family proteins to form functional ternary complexes on a B-cell-specific promoter. (1996)

The paired box transcription factor Pax-5 (B-cell-specific activator protein) is a key regulator of lineage-specific gene expression and differentiation in B-lymphocytes. We show that Pax-5 functions as a cell type-specific docking protein that facilitates binding of the early B-cell-specific mb-1 promoter by proteins of the Ets proto-oncogene family. Transcriptional activity of the mb-1 promoter in pre-B-cells is critically dependent on binding sites for Pax-5:Ets complexes. Ternary complex assembly requires only the Pax-5 paired box and ETS DNA-binding domains. Mutation of a single base pair in the ternary complex binding site allows for independent binding by Ets proteins but, conversely, inhibits the binding of Pax-5 by itself. Strikingly, the mutation reverses the pattern of complex assembly: Ets proteins recruit Pax-5 to bind the mutated sequence. Recruitment of Net and Elk-1, but not SAP1a, by Pax-5 defines a functional difference between closely related Ets proteins. Replacement of a valine (V68) in the ETS domain of SAP1a by aspartic acid (as found in c-Ets-1, Elk-1, and Net) enhanced ternary complex formation by more than 60-fold. Together, these observations define novel transcription factor interactions that regulate gene expression in B cells.
KSim: a stable and efficient RKC simulator for capturing on-chip inductance effect (2002)

On-chip inductance extraction is difficult due to the global effect of inductance, and simulating the resulting dense partial inductance matrix is even more difficult. Furthermore, it is well known that simply discarding smallest terms to sparsify the inductance matrix can render the partial inductance matrix indefinite and result in an unstable circuit model. Recently a new circuit element, K, has been introduced to capture the global effect of inductance by evaluating a corresponding sparse K matrix. However, the reason that K has such local properties is not clear, and the positive semi-definiteness of the corresponding sparse K matrix is not proved. In this paper, we present the physical interpretation of K. Based on the physical interpretation, we explain why the faraway mutual K can be ignored (locality) and prove that after ignoring faraway mutual K, the resultant K matrix is positive definite (stability). Together with a RKC equivalent circuit model, the locality and stability enables us to simulate an RKC circuit directly and efficiently for real circuits. A new circuit simulation tool, KSim, has been developed by incorporating the new circuit element K into Berkeley SPICE. The RKC simulation matches better with the full partial inductance matrix simulation with significant less computing time and memory usage, compared to other proposed methods, such as the shift-truncate method.
Weakly Supervised Training of Semantic Parsers (2018)

We present a method for training a semantic parser using only a knowledge base and an unlabeled text corpus, without any individually annotated sentences. Our key observation is that multiple forms of weak supervision can be combined to train an accurate semantic parser: semantic supervision from a knowledge base, and syntactic supervision from dependencyparsed sentences. We apply our approach to train a semantic parser that uses 77 relations from Freebase in its knowledge representation. This semantic parser extracts instances of binary relations with state-of-the art accuracy, while simultaneously recovering much richer semantic structures, such as conjunctions of multiple relations with partially shared arguments. We demonstrate recovery of this richer structure by extracting logical forms from natural language queries against Freebase. On this task, the trained semantic parser achieves 80% precision and 56% recall, despite never having seen an annotated logical form.
Effects of antagonists to high-threshold calcium channels upon spinal mechanisms of pain, hyperalgesia and allodynia (2000)

High-threshold voltage-dependent calcium channels enable calcium ions to enter neurons upon depolarization and thereby influence synaptic mediator/receptor systems, membrane excitability levels, second and third messenger concentration, and gene expression. These phenomena underlie several processes including those of normal nociception and of hyperalgesia and allodynia. The present article deals with the role of spinal L-, N- and P/Q-type calcium channels in short-lasting nociception as well as in the hyperalgesia and allodynia elicited by chemical irritants of peripheral nociceptors, inflammatory and mechanical lesions of peripheral tissues, and lesions of peripheral nerves. The studies summarized herein are based on the spinal delivery of specific antagonists to high-threshold calcium channels, and reveal that blockade of L-type, P/Q-type and, particularly, N-type channels can prevent, attenuate, or both, subjective pain as well as primary and/or secondary hyperalgesia and allodynia in a variety of experimental and clinical conditions.
Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections (2000)

Many gram-positive bacteria covalently tether their surface adhesins to the cell wall peptidoglycan. We find that surface proteins of Staphylococcus aureus are linked to the cell wall by sortase, an enzyme that cleaves polypeptides at a conserved LPXTG motif. S. aureus mutants lacking sortase fail to process and display surface proteins and are defective in the establishment of infections. Thus, the cell wall envelope of gram-positive bacteria represents a surface organelle responsible for interactions with the host environment during the pathogenesis of bacterial infections.
An iron-catalysed hydrosilylation of ketones (2007)

The combination of Fe(OAc)2 and multi-nitrogen-based ligands such as N,N,N',N'-tetramethyethylenediamine, bis-tert-butyl-bipyridine, or bis(oxazolinyl)pyridine can efficiently catalyse hydrosilylation of ketones to give the corresponding alcohols in high yields including asymmetric catalysis.