gebhart/patrepeval huggingface dataset:
Immobilization of Human Thrombomodulin onto Biomaterials (1994)

Human thrombomodulin (hTM), which is a newly described endothelial cell associated protein that functions as a potent natural anticoagulant by converting thrombin from a procoagulant protease to an anticoagulant, was immobilized on to various substrates by two immobilization methods. As the substrates of immobilization, poly(acrylic acid) surface grafted poly(ethylene) (PAAc-g-PE) film, poly(vinylamine) surface grafted poly(ethylene) film, and PAAc surface grafted nylon were used. For immobilization, simultaneous preactivation methods were used. The effect of the immobilization reaction on hTM activities, the comparison of the activities of immobilized hTM with those of free hTM, and the effect of the thrombin incorporation on antithrombogenic activity were studied. The hTM immobilized onto PAAc-g-PE by preactivation showed the highest antithrombogenic activity. The thrombin incorporation affected protein C activation activity but not the fibrinogen clotting time. hTM immobilized nylon showed greater antithrombogenicity in vitro.
THE BACTERIAL PHOSPHOENOL-PYRUVATE: GLYCOSE PHOSPHOTRANSFERASE SYSTEM (1990)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Germinação de sementes de café (Coffea arabica L.) submetidas a diferentes concentrações e tempos de embebição em celulase (2003)

As sementes de café germinam lentamente em razão de a semeadura ser feita logo após a colheita, o que coincide com o período frio do ano, e também por causa de o pergaminho constituir uma barreira que dificulta a absorção de água pela semente. Objetivando avaliar o efeito da aplicação exógena da enzima celulase sobre a germinação de sementes de café, foi realizado o experimento com sementes de café, cultivar Acaiá do Cerrado, imersas em solução de celulase em diferentes concentrações (0; 1,6; 3,2 e 6,4 g.L-1 em tampão citrato de potássio 0,05 M e pH 4,8), por 0, 72 e 144 horas. Observou-se que a enzima celulase na concentração de 6,4 g.L-1 proporcionou maior índice de velocidade de germinação (IVG) e porcentagem de germinação, quando as sementes ficam em contato com a solução por 144 horas.
Human and mouse Mx proteins inhibit different steps of the influenza virus multiplication cycle (1992)

Human MxA and mouse Mx1 are interferon-induced proteins capable of inhibiting the multiplication of influenza virus. MxA protein is localized in the cytoplasm, whereas Mx1 protein accumulates in the nucleus. Taking advantage of stably transfected cell lines that constitutively express either MxA or Mx1 protein, we examined the steps at which these proteins block influenza A viruses. In infected cells expressing MxA protein, all viral mRNAs synthesized as a result of primary transcription in the nucleus by the virion-associated RNA polymerase accumulated to normal levels. These primary viral transcripts were polyadenylated, were active in directing viral protein synthesis in vitro, and appeared to be efficiently transported to the cell cytoplasm. Yet viral protein synthesis and genome amplification were strongly inhibited, suggesting that MxA protein interfered with either intracytoplasmic transport of viral mRNAs, viral protein synthesis, or translocation of newly synthesized viral proteins to the cell nucleus. However, in infected cells expressing Mx1 protein, the concentrations of the longest primary transcripts encoding the three influenza virus polymerase proteins PB1, PB2, and PA were at least 50-fold reduced. Accumulation of the shorter primary transcripts encoding the other viral proteins was also inhibited but to a lesser extent. These results demonstrate that the mouse Mx1 protein interferes with primary transcription of influenza virus in the nucleus, whereas the human MxA protein inhibits a subsequent step that presumably takes place in the cytoplasm of infected cells.
Direct arylation of nitrobenzenes and of heterocycles via an oxygen analogue of the benzidine rearrangement (1977)

Benzyl N-hydroxy-N-phenylcarbamate (1) and N-phenylbenzohydroxamic acid (10) react with active halogenobenzenes to give derivatives of 4-amino-4′-hydroxybiphenyl. The reactions of compound (1) or (10) with certain 2-chloro-pyridines, -pyrimidines, and -pyrazines result in introduction of the 4-aminophenyl group at position 5 of the heterocycle. The scope and limitations of the reaction are discussed.
Increased Expression of CCL2 in Insulin-Producing Cells of Transgenic Mice Promotes Mobilization of Myeloid Cells From the Bone Marrow, Marked Insulitis, and Diabetes (2008)

OBJECTIVE—To define the mechanisms underlying the accumulation of monocytes/macrophages in the islets of Langerhans. RESEARCH DESIGN AND METHODS—We tested the hypothesis that macrophage accumulation into the islets is caused by overexpression of the chemokine CCL2. To test this hypothesis, we generated transgenic mice and evaluated the cellular composition of the islets by immunohistochemistry and flow cytometry. We determined serum levels of CCL2 by enzyme-linked immunosorbent assay, determined numbers of circulating monocytes, and tested whether CCL2 could mobilize monocytes from the bone marrow directly. We examined development of diabetes over time and tested whether CCL2 effects could be eliminated by deletion of its receptor, CCR2. RESULTS—Expression of CCL2 by β-cells was associated with increased numbers of monocytes in circulation and accumulation of macrophages in the islets of transgenic mice. These changes were promoted by combined actions of CCL2 at the level of the bone marrow and the islets and were not seen in animals in which the CCL2 receptor (CCR2) was inactivated. Mice expressing higher levels of CCL2 in the islets developed diabetes spontaneously. The development of diabetes was correlated with the accumulation of large numbers of monocytes in the islets and did not depend on T- and B-cells. Diabetes could also be induced in normoglycemic mice expressing low levels of CCL2 by increasing the number of circulating myeloid cells. CONCLUSIONS—These results indicate that CCL2 promotes monocyte recruitment by acting both locally and remotely and that expression of CCL2 by insulin-producing cells can lead to insulitis and islet destruction.
La evolución de la Educación Jurídica Clínica en España. (2015)

La educacion juridica clinica tiene importancia como instrumento de transformacion de la ensenanza del Derecho en tres aspectos (Garcia Anon, 2014b, 2014b). En primer lugar, en el cambio de los metodos de ensenanza. En segundo lugar, como una de las formas en las que se reconoce el papel de la Universidad en y para la sociedad; y, en tercer lugar, como una manera de colaborar en el derecho acceso a la justicia y los derechos, desde la perspectiva de la formacion, pero no solo de esa manera.
Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody. (1986)

A monoclonal antibody, MOMA-1, is described that recognizes the marginal metallophilic macrophages in the mouse spleen. The cells are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of the marginal zone. They show high non-specific esterase activity and can be distinguished from the marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake. The relationship of MOMA-1-positive cells with other macrophage populations is discussed.
Translocation to the Periplasm and Signal Sequence Cleavage of Preapocytochrome c Depend on Sec and Lep, but not on the ccm gene products (1997)

Post-translational maturation of soluble cytochrome c includes translocation of the precursor polypeptide and heme through the cytoplasmic membrane, proteolytic cleavage of the signal sequence, and covalent attachment of heme. Specific genes for cytochrome c maturation (ccmABCDEFGH in Escherichia coli) are required for holocytochrome c formation, among them genes encoding an ABC transporter (ccmABC). We investigated the requirements of apocytochrome translocation to the periplasm and characterized specific intermediates of the cytochrome c maturation pathway. Apocytochrome precursor was present in the membrane fraction. Translocation of the polypeptide was independent of ccm gene products, but dependent on a functional secretion machinery, as shown by accumulation of preapocytochrome c in the membranes of secA and secY mutants. After translocation, cleavage of the signal sequence allowed the release of apocytochrome into the periplasm, where heme was bound in a ccm-dependent manner. By contrast, non-cleaved holocytochrome c containing covalently bound heme accumulated in the membranes of a lepB mutant, which indicated that signal sequence cleavage and heme attachment are independent steps in the cytochrome c maturation pathway.
Drug targeting with polyalkylcyanoacrylate nanoparticles:in vitroactivity of primaquine-loaded nanoparticles against intracellularLeishmania donovani (1992)

The efficacy of primaquine-loaded polyisohexylcyanoacrylate (PIHCA) nanoparticles was evaluated using J774G8 macrophage-like cells infected with Leishmania donovani: as an in vitro model of visceral leishmaniasis. The in vitro antileishmanial activity of primaquine-loaded nanoparticles showed a 21-fold increase in ED50 compared with free primaquine. Although unloaded PIHCA nanoparticles also exhibited a significant anti-leishmanial effect, the loaded nanoparticles showed a synergistic effect compared with a mixture of unloaded nanoparticles and free primaquine at equivalent concentrations. Primaquine release and isohexanol production were evaluated in a lysosomal fraction; the correlation of both with protein concentration and the rapid drug release indicate the processes are associated with an enzymatic degradation. The results indicate that PIHCA and other polyalkylcyanoacrylates may be useful for targeting drugs at intracellular Leishmania, and that the unloaded carrier itself could be of interest in experimental chemotherapy of leishmaniasis.
Post‐Earnings Announcement Drift and the Dissemination of Predictable Information* (1999)

Abstract Building on the work of Bernard and Thomas 1990, we develop a model to infer the degree to which the information in an earnings announcement is incorporated into investors' expectations for the subsequent earnings announcement at any point in time between the two announcements. We are unable to reject the null hypothesis that investors' earnings expectations are based on a seasonal random walk and reflect none of the implications of the immediately prior earnings announcement up to 15 trading days after that announcement. By mid‐quarter, expectations are significantly more sophisticated than a seasonal random walk. Two trading days before the next earnings announcement, as much as one half of the information in the prior earnings announcement is reflected in earnings expectations. We also find that the dissemination of information, albeit predictable information, speeds the incorporation of prior earnings information into earnings expectations. Our results suggest that as information about future earnings that could have been discerned from the earlier announcements (because past earnings surprises predict future ones) is disseminated in a more transparent form, investors revise their earnings expectations to reflect this information. Thus, the investors' expectations appear to incorporate more and more of the serial correlation in earnings surprises as the quarter progresses, even though they do not consider per se the serial correlation in earnings surprises in forming their expectations.
Impaired Cognitive Function and Hippocampal Neurogenesis following Cancer Chemotherapy (2012)

Our results show that chronic treatment with either of two commonly used chemotherapeutic agents impairs cognitive ability and suggest that strategies to prevent or repair disrupted hippocampal neurogenesis may be effective in ameliorating this serious side effect in cancer survivors.
Axial Wall Slits Effect on the Helical Flow in the Gap between two Concentric Cylinders (2012)

The helical flow regime was investigated by using DPIV when the rotating Reynolds number is small. The wall slits were azimuthally located along the inner wall of outer cylinder and the slits number of each model was 9 and 18, another plain wall model was also studied for comparison purpose. The helical vortex flow regime can be observed in all the three models. The negative temperature gradients determine the direction of the rotation and movement of the helical vortex. But the helical wavy vortex flow can only be found in the plane and 9-slit models. And the result showed that the existence of slit wall accelerated the transition process.
Trellis coded polarization shift keying modulation for digital optical communications (1995)

The application of the well-known technique of trellis coded modulation to coherent optical communications using polarization shift keying (POLSK) is described and analyzed. The resulting receiver is formed by a front-end which performs the heterodyne detection and the Stokes parameter extraction, cascaded with an electronic Viterbi processor operating the maximum likelihood estimate of the transmitted sequence. Results in terms of the error event probability using optimum as well as a simpler suboptimum branch metric show power gains of the order of 3-4 dB, at the expense of a reasonable increase in complexity, only concerning the processing in the electronic domain. These coding gains are not lost even in the presence of high levels of phase noise, to which POLSK in general is highly insensitive.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Mapping of metastasis suppressor genes for prostate cancer by microcell-mediated chromosome transfer. (2000)

This assay system is useful to identify metastasis suppressor gene (s) for prostate cancer.
The Human Growth Hormone Gene Family: Nucleotide Sequences Show Recent Divergence and Predict a New Polypeptide Hormone (1982)

The nucleotide sequences of three nonallelic human genomic DNA fragments which each contain one member of the growth hormone gene family are presented. These genes code for the known polypeptide hormones, growth hormone (hGH), chorionic somatomammotropin (hCS), and a yet unknown protein which differs from hGH in 13 positions. Each gene is structured into five exons, the four introns occurring at identical positions. Reflecting recent gene divergence, 90-95% sequence homology is seen in exons, introns, 5', and immediate 3' nontranscribed regions. The regions downstream of the polyadenylation sites in the genes for hGH and its variant, but not for hCS, contain members of an Alu family repeat sequence.
Absence of mutations in the β‐catenin and adenomatous polyposis coli genes in papillary and follicular thyroid carcinomas (2001)

beta-Catenin has multiple functions both in intercellular adhesion and in signal transduction. As a signaling molecule, mutations in exon 3 of the beta-catenin gene stabilize this protein in the cytoplasm. Subsequently, accumulated beta-catenin protein translocates to nuclei with T-cell factor-4, and upregulates transcriptional activity of the target genes involved in carcinogenesis. Mutations in exon 3 of the beta-catenin gene have been detected in various carcinomas. We examined immunolocalization of beta-catenin protein and mutations in the beta-catenin and adenomatous polyposis coli (APC) genes in papillary carcinoma (25 cases), follicular carcinoma (two cases), and benign thyroid tumor (29 cases). We detected no mutation in exon 3 of the beta-catenin gene in both malignant and benign thyroid tumors by polymerase chain reaction (PCR) and direct sequencing. No mutations in the mutation cluster region of APC were found in any tumor samples analyzed. Immunohistochemically, beta-catenin showed membranous localization in most specimens. These results suggest that mutations of the beta-catenin and APC genes are rare and that activation of the Wnt signaling pathway may not contribute to pathogenesis in human papillary and follicular thyroid carcinomas.
Improving Menu Interaction for Cluttered Tabletop Setups with User-Drawn Path Menus (2007)

Many digital tabletop systems have a graphical user interface (GUI) that features context (or pop-up) menus. While linear and pie menus are commonly used for direct pen and touch interaction, their appearance can be problematic on a digital tabletop display, where physical objects might occlude menu items. We propose a user-drawn path menu, that appears along a custom path to avoid such occlusions. This paper introduces four different metaphors for user-drawn context menus: the Fan Out Menu, the Card Deck Menu, the Pearl String Menu, and the Trail Menu. It also presents the results we acquired from a user study, where participants were able to work faster when using our user-drawn menus, on cluttered tabletop setups.
Sjogren's Systemic Clinical Activity Index (SCAI) a systemic disease activity measure for use in clinical trials in primary Sjogren's syndrome (2007)

This initial evaluation supports the potential for the SCAI as a tool for systemic activity assessment in patients with PSS but additional work is required to assess sensitivity to change in clinical therapeutic trials.
Variation of Oleanolic and Ursolic Acid in the Flesh of Persimmon Fruit among Different Cultivars (2010)

Oleanolic acid (OA) and ursolic acid (UA) are important bioactive components in many plants, including persimmon (Diospyros kaki L.). The present work was carried out to determine OA and UA contents in the flesh of persimmon fruit from 32 cultivars, including 23 astringent and 9 non-astringent ones, by applying high performance liquid chromatography (HPLC) with UV detection. Both OA and UA were present in all of the investigated cultivars, except for three, 'Hiratanenashi', 'Ribenhongshi' and 'Matsumotowase'. The OA content ranged from traces to 88.57 μg/g FW, and that of UA were between traces and 27.64 μg/g FW.
Location of the Trans -Activating Region on the Genome of Human T-Cell Lymphotropic Virus Type III (1985)

The retrovirus involved in acquired immune deficiency syndrome (HTLV-III/LAV) contains a region that is necessary for stimulation of gene expression directed by the viral long terminal repeat. This region is located between nucleotides 5365 and 5607, immediately 5' to the envelope gene. A doubly-spliced message containing this region could encode an 86-amino acid protein with structural features similar to those of nucleic acid-binding proteins.
Role of IL-1β in type 2 diabetes (2010)

Monotherapy or add-on therapy targeting IL-1beta in type 2 diabetes holds promise for long-term benefits in glycemic control and possibly reducing cardiovascular events.
Local Administration of a Synthetic Cell-Penetrating Peptide Antagonizing TrkA Function Suppresses Inflammatory Pain in Rats (2010)

Novel agents that inhibit nerve growth factor signaling are required for the treatment of inflammatory pain. The present study investigated the effect of local administration of inhibitory peptide of TrkA (IPTRK3), a synthetic cell-penetrating peptide that antagonizes TrkA function, in complete Freund's adjuvant (CFA)-induced hyperalgesia in rats. Three hours after subcutaneous injection of CFA into the plantar surface of the rat's left hind paw, 10 mM IPTRK3 was injected at the same site. Thermal and mechanical hyperalgesia were tested in the ipsilateral hind paw until 7 days after CFA injection. The ipsilateral dorsal root ganglion (DRG) was dissected out for immunohistochemical analysis of transient receptor potential vanilloid subfamily member 1 (TRPV1) channels and TrkA. Local injection of this peptide significantly suppressed both thermal and mechanical hyperalgesia produced by CFA and also significantly reduced TRPV1 expression at the DRG. These results suggest that local administration of IPTRK3 is likely effective in the treatment of inflammatory pain in rats.
Association of Mu-Opioid Receptor Variants and Response to Citalopram Treatment in Major Depressive Disorder (2010)

These results suggest that rates of response to antidepressants and consequent remission from major depressive disorder are influenced by variation in the mu-opioid receptor gene as a result of either an effect on placebo response or true pharmacologic response.
On limits of wireless communications when using multiple dual-polarized antennas (2003)

In this paper, capabilities of wireless communication systems using multiple dual-polarized antennas at receiver and transmitter side are examined in terms of channel capacity. Therefore an intrinsic channel description including polarization effects is been derived, which allows the modeling of transmission between array antennas which are composed of closely spaced and arbitrarily oriented antennas in a fading environment. Although only two orthogonal polarization can be accessed in antenna farfield, it is shown, that the use of orthogonal oriented antennas may provide high capacities even for closely spaced antennas.
Noninvasive Imaging of PSMA in Prostate Tumors with 89Zr-Labeled huJ591 Engineered Antibody Fragments: The Faster Alternatives (2014)

Engineered antibody fragments offer faster delivery with retained tumor specificity and rapid clearance from nontumor tissues. Here, we demonstrate that positron emission tomography (PET) based detection of prostate specific membrane antigen (PSMA) in prostatic tumor models using engineered bivalent antibodies built on single chain fragments (scFv) derived from the intact antibody, huJ591, offers similar tumor delineating properties but with the advantage of rapid targeting and imaging. (89)Zr-radiolabeled huJ591 scFv (dimeric scFv-CH3; (89)Zr-Mb) and cysteine diabodies (dimeric scFv; (89)Zr-Cys-Db) demonstrated internalization and similar Kds (∼2 nM) compared to (89)Zr-huJ591 in PSMA(+) cells. Tissue distribution assays established the specificities of both (89)Zr-Mb and (89)Zr-Cys-Db for PSMA(+) xenografts (6.2 ± 2.5% ID/g and 10.2 ± 3.4% ID/g at 12 h p.i. respectively), while minimal accumulation in PSMA(-) tumors was observed. From the PET images, (89)Zr-Mb and (89)Zr-Cys-Db exhibited faster blood clearance than the parent huJ591 while tumor-to-muscle ratios for all probes show comparable values across all time points. Ex vivo autoradiography and histology assessed the distribution of the probes within the tumor. Imaging PSMA-expressing prostate tumors with smaller antibody fragments offers rapid tumor accumulation and accelerated clearance; hence, shortened wait periods between tracer administration and high-contrast tumor imaging and lower dose-related toxicity are potentially realized.
Artificial Hairy Surfaces with a Nearly Perfect Hydrophobic Response (2010)

A nearly perfect hydrophobic interface by dint of mimicking hairs of arthropods was achieved for the first time. These Gamma-shape artificial hairs were made via a membrane casting technique on polypropylene substrates. This extreme hydrophobicity merely arises from microstructure modification, and no further chemical treatments are needed. The ultralow adhesion to water droplets was evaluated through video assessment, and it is believed to be attributed to the mechanical response of the artificial hairs. The principle of this fabrication technique is accessible and is expected to be compatible with large-area fabrication of superhydrophobic interfaces.