gebhart/patrepeval huggingface dataset:
Conjunctival epithelial cell differentiation on amniotic membrane. (1999)

RCEs cultured on AM primarily exhibit a nongoblet conjunctival epithelial phenotype. Epithelial stratification and cell polarity, features essential for epithelial differentiation, are promoted by air-lifting. This culture model will be useful for studying how growth and differentiation of conjunctival epithelial cells can be modulated further.
Anti-thymocyte globulin (ATG) prevents autoimmune encephalomyelitis by expanding myelin antigen-specific Foxp3+ regulatory T cells (2007)

The T cell-depleting polyclonal antibody, anti-thymocyte globulin (ATG) has long been used in organ transplantation to treat acute rejection episodes. More recently, it is also being used as part of an induction regimen to protect allografts. It has been proposed that ATG might deplete effector T cells (T-effs) while sparing regulatory T cells (T-regs). In order to test whether ATG is effective in autoimmune disease, we used Foxp3gfp 'knock-in' mice in combination with a myelin oligodendrocyte glycoprotein (MOG)(35-55)/IA(b) tetramer to study more closely the effect of ATG treatment on antigen-specific T cell responses in vivo during MOG-induced experimental autoimmune encephalomyelitis (EAE), an animal model for Multiple Sclerosis. ATG treatment enhanced the expansion of MOG-specific T-regs (CD4(+)Foxp3(+)) in MOG-immunized mice. T-effs were depleted, but on a single-cell basis, the effector function of residual T-effs was not compromised by ATG. Thus, ATG tipped the balance of T-effs and T-regs and skewed an auto-antigen-specific immune reaction from a pathogenic T cell response to a potentially protective T-reg response. In both acute and relapsing remitting disease models, ATG treatment resulted in the attenuation from EAE, both in a preventive and early therapeutic setting. We conclude that ATG treatment enforces the development of a dominant immunoregulatory environment which may be advantageous for the treatment of T cell-driven autoimmune diseases.
Isolation and Characterization of Novel Geldanamycin Analogues (2004)

New geldanamycin analogues with novel structures arising from direct microbial bioconversion and a genetically engineered geldanamycin producer were isolated and characterized. Three compounds, 15-hydroxygeldanamycin, a tricyclic geldanamycin analog (KOSN-1633), and methyl-geldanamycinate), were isolated after geldanamycin was added to a growing culture of the herbimycin producing strain-Streptomyces hygroscopicus AM-3672. Two related compounds, 17-formyl-17-demethoxy-18-O,-21-O-dihydrogeldanamycin and 17-hydroxymethyl-17-demethoxygeldanamycin were isolated from S. hygroscopicus NRRL 3602/pKOS279-78, a geldanamycin-producing strain containing various genes isolated from S. hygroscopicus AM-3672. Compared with geldanamycin, these five new compounds exhibited reduced cytotoxicity against SKBr3 cancer cells.
Authentication, Authorization and File Synchronization in Hybrid Cloud: On Case of Google Docs, Hadoop and Linux Local Hosts (2013)

Cloud computing brings more flexible options for the Small and Medium Business (SMB). With the help of public cloud, such as Google Docs, the SMBs are able to share their business information in lower cost but higher efficiency. Integrity between local storages and the clouds is a critical issue which often messes up the SMBs' digital assets in a hybrid environment. The downside makes SMBs reluctant to embrace cloud technology, though they may benefit from it in the long-term. The drawback comes from two aspects: "the information chaos" and "the management crisis". The chaos originates from the possibility of information inconsistency among replicas in different places. On the other hand, the managerial crisis lies in the non-trustable access to the digital assets. Our research refers to the de facto open standards, viz. OpenID and O'Auth, to solve the problem by identity authentication and access right authorization on the hybrid cloud. The mechanisms are implemented for the Linux-based local host as well as MS-Windows-based computers, a private cloud constructed with Hadoop, and the Google Apps as a public cloud scenario. Users can launch the login from whatever a local host and then have Google Apps, Facebook and Twitter deal with access right permissions on the respective clouds. At last, we validate, according to CMMI, the functionality, reliability, integrity, etc. of the mechanisms to show the superiority and applicability of our approach.
Three‐dimensional multispectral optoacoustic mesoscopy reveals melanin and blood oxygenation in human skin in vivo (2015)

Optical imaging plays a major role in disease detection in dermatology. However, current optical methods are limited by lack of three-dimensional detection of pathophysiological parameters within skin. It was recently shown that single-wavelength optoacoustic (photoacoustic) mesoscopy resolves skin morphology, i.e. melanin and blood vessels within epidermis and dermis. In this work we employed illumination at multiple wavelengths for enabling three-dimensional multispectral optoacoustic mesoscopy (MSOM) of natural chromophores in human skin in vivo operating at 15-125 MHz. We employ a per-pulse tunable laser to inherently co-register spectral datasets, and reveal previously undisclosed insights of melanin, and blood oxygenation in human skin. We further reveal broadband absorption spectra of specific skin compartments. We discuss the potential of MSOM for label-free visualization of physiological biomarkers in skin in vivo.
An Alternative Reaction Course in O-Glycosidation with O-Glycosyl Trichloroacetimidates as Glycosyl Donors and Lewis Acidic Metal Salts as Catalyst: Acid–Base Catalysis with Gold Chloride-Glycosyl Acceptor Adducts (2015)

Gold(III) chloride as catalyst for O-glycosyl trichloroacetimidate activation revealed low affinity to the glycosyl donor but high affinity to the hydroxy group of the acceptor alcohol moiety, thus leading to catalyst-acceptor adduct formation. Charge separation in this adduct, increasing the proton acidity and the oxygen nucleophilicity, permits donor activation and concomitant acceptor transfer in a hydrogen-bond mediated S(N)2-type transition state. Hence, the sequential binding between acceptor and catalyst and then with the glycosyl donor enables self-organization of an ordered transition-state. This way, with various acceptors, even at temperatures below -60 °C, fast and high yielding glycosidations in high anomeric selectivities were recorded, showing the power of this gold(III) chloride acid-base catalysis. Alternative reaction courses via hydrogen chloride or HAuCl4 activation or intermediate generation of glycosyl chloride as the real donor could be excluded. With partially O-protected acceptors, prone to bidentate ligation to gold(III) chloride, particularly high reactivities and anomeric selectivities were observed. Gold(I) chloride follows the same catalyst-acceptor adduct driven acid-base catalysis reaction course.
Biological functions of iduronic acid in chondroitin/dermatan sulfate (2013)

The presence of iduronic acid in chondroitin/dermatan sulfate changes the properties of the polysaccharides because it generates a more flexible chain with increased binding potentials. Iduronic acid in chondroitin/dermatan sulfate influences multiple cellular properties, such as migration, proliferation, differentiation, angiogenesis and the regulation of cytokine/growth factor activities. Under pathological conditions such as wound healing, inflammation and cancer, iduronic acid has diverse regulatory functions. Iduronic acid is formed by two epimerases (i.e. dermatan sulfate epimerase 1 and 2) that have different tissue distribution and properties. The role of iduronic acid in chondroitin/dermatan sulfate is highlighted by the vast changes in connective tissue features in patients with a new type of Ehler-Danlos syndrome: adducted thumb-clubfoot syndrome. Future research aims to understand the roles of the two epimerases and their interplay with the sulfotransferases involved in chondroitin sulfate/dermatan sulfate biosynthesis. Furthermore, a better definition of chondroitin/dermatan sulfate functions using different knockout models is needed. In this review, we focus on the two enzymes responsible for iduronic acid formation, as well as the role of iduronic acid in health and disease.
Real-Time Reverse Transcription-PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens (2007)

The mumps virus is a negative-strand RNA virus in the family Paramyxoviridae. Mumps infection results in an acute illness with symptoms including fever, headache, and myalgia, followed by swelling of the salivary glands. Complications of mumps can include meningitis, deafness, pancreatitis, orchitis, and first-trimester abortion. Laboratory confirmation of mumps infection can be made by the detection of immunoglobulin M-specific antibodies to mumps virus in acute-phase serum samples, the isolation of mumps virus in cell culture, or by detection of the RNA of the mumps virus by reverse transcription (RT)-PCR. We developed and validated a multiplex real-time RT-PCR assay for rapid mumps diagnosis in a clinical setting. This assay used oligonucleotide primers and a TaqMan probe targeting the mumps SH gene, as well as primers and a probe that targeted the human RNase P gene to assess the presence of PCR inhibitors and as a measure of specimen quality. The test was specific, since it did not amplify a product from near-neighbor viruses, as well as sensitive and accurate. Real-time RT-PCR results showed 100% correlation with results from viral culture, the gold standard for mumps diagnostic testing. Assay efficiency was over 90% and displayed good precision after performing inter- and intraassay replicates. Thus, we have developed and validated a molecular method for rapidly diagnosing mumps infection that may be used to complement existing techniques.
Collections & Collectors: 39. The Sedgwick Museum Cambridge (1981)

The Sedgwick Museum houses major palaeontological collections of international importance associated with one of the World's oldest geological Professorships. Its collections, because of their early start (certain specimens date back to 17th century collections),^ their rapid growth throughout the 19th century and the very active palaeontological and biostratigraphical research which has centred on them from Adam Sedgwick's time onwards, include a great many type, figured and described specimens and represent many of the classic researches of British geology as well as including important foreign material.
Fast silicon-on-silicon optoelectronic router based on a BMFET device (2000)

In this paper, a new configuration for an optoelectronic router is presented. The device is composed by a single-mode input waveguide, a two-mode active section where the light steering by means of mode mixing between the fundamental and the first higher order mode is achieved. A terminal Y-branch is placed to separate the output channels. Active modulation is achieved by means of a three-terminal electronic device, which injects and controls the free carrier plasma inside the active region. The usage of a three-terminal device allows extremely fast dynamic response. Switching times of few nanoseconds are obtained together with an average crosstalk of -11 dB.
Design Characteristics of the K=800 Superconducting Cyclotron at M.S.U. (1979)

Design work on the proposed K=800, KF= 400, superconducting cyclotron at MSU has progressed to the point where overall machine characteristics are firmly established. The main purpose1 of the machine is to serve as a booster for the K=500 cyclotron, now under construction. In this respect, let us recall that the design goal is to achieve maximum beam energies of 200 MeV/n for fully stripped light ions, and typically 30-40 MeV/n for heavy ions like uranium. Energy variability down to about 50 MeV/n and 4-5 MeV/n, again for light and heavy ions respectively, is also required to ensure some overlap with beam energies delivered by the K = 500 in stand-alone mode.
Detection of progesterone receptor forms A and B by immunohistochemical analysis (2001)

Most PR specific antibodies tested failed to detect PRB in formalin fixed tissue by immunohistochemical techniques, despite their ability to do so by immunoblot analysis. These observations suggest that there are conformational differences between PRA and PRB that mask epitopes on the PRB protein recognised by most anti-PR antibodies. The selection of antibodies that recognise both PRB and PRA in formalin fixed tissue is essential for the accurate evaluation of PR positivity in clinical specimens.
The application of adaptive filters for motion prediction in visually tracked laparoscopic surgery (2007)

More and more research teams propose robotic systems to control the position of the laparoscope. In many of them the visual tracking principle is applied. However as experiments have shown tracked markers may often be obscured during a surgery. Within the article a study on the possibility of the application of a predictive algorithm to anticipate the position of a chirurgical tool is presented. This system would generate an estimate of the position whenever tracked tool’ s marker is obscured. As its basis the LMS algorithm was chosen, to which supplementary modifications were introduced. Experimental simulations prove that the algorithm greatly reduces the amount of missing samples during real time operation. Obtained predicted positions of the chirurgical tool reasonably approximate its true position.
Effects of engineering complementary charged residues into the hydrophobic subunit interface of tyrosyl-tRNA synthetase (1987)

Wild-type tyrosyl-tRNA synthetase (TyrTS) from Bacillus stearothermophilus is a symmetrical dimer. Four different heterodimeric enzymes have been produced by site-directed mutagenesis at the subunit interface so that the monomers are linked by a potential salt bridge in a hydrophobic environment. The two Phe-164 residues of wild-type TyrTS are on the axis of symmetry and interact in a hydrophobic region of the subunit interface. Mutation of Phe-164 to aspartate or glutamate in full-length TyrTS and to lysine or arginine in an active truncated enzyme (delta TyrTS) induces reversible dissociation of the enzyme into inactive monomers. Mixing mutants in equimolar amounts produces four different heterodimers: TyrTS(Asp-164)-delta TyrTS(Lys-164); TyrTS(Asp-164)-delta TyrTS(Arg-164); TyrTS(Glu-164)-delta TyrTS(Lys-164); TyrTS(Glu-164)-delta TyrTS(Arg-164). A general method is derived for analyzing the kinetics of dimeric enzymes that reversibly dissociate into inactive subunits. Application to mutants of TyrTS allows estimation of dissociation constants (Kd values) for the dimers. At pH 7.8, the heterodimers have Kd values of 6-14 microM, whereas for homodimers Kd = 120-4000 microM. These values decrease to about 30 microM for homodimers of TyrTS(Asp-164), TyrTS(Glu-164), and delta TyrTS(Lys-164) when the pH favors uncharged forms of the side chains at position 164. Each of the four salt bridges engineered into the hydrophobic subunit interface of TyrTS appears, therefore, to be weak. These engineered salt bridges may be compared with naturally occurring ones. In the latter, there are complementary interactions between the charges in the salt bridge with polar groups in the protein.(ABSTRACT TRUNCATED AT 250 WORDS)
Membranes Containing Oriented Supramolecular Transport Channels (2000)

The surface-induced alignment of crown ether units bearing amphiphiles into columnar structures within the pores of track-etched membranes is reported (see Figure). The ion-transport properties of these membranes are characterized by salt diffusion experiments. It is found that the membranes containing oriented supramolecular channels display strongly enhanced transport rates compared to those with non-oriented channels.
Synthesis of Triacylglycerols by the Acyl-Coenzyme A:Diacyl-Glycerol Acyltransferase Dga1p in Lipid Particles of the Yeast Saccharomyces cerevisiae (2002)

The terminal step of triacylglycerol (TAG) formation in the yeast Saccharomyces cerevisiae is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase (DAGAT). In this study we demonstrate that the gene product of YOR245c, Dga1p, catalyzes a major yeast DAGAT activity which is localized to lipid particles. Enzyme measurements employing a newly established assay containing radioactively labeled diacylglycerol (DAG) as a substrate and unlabeled palmitoyl-CoA as a cosubstrate revealed a 70- to 90-fold enrichment of DAGAT in lipid particles over the homogenate but also a 2- to 3-fold enrichment in endoplasmic reticulum fractions. In a dga1 deletion strain, the DAGAT activity in lipid particles is dramatically reduced, whereas the activity in microsomes is affected only to a minor extent. Thus, we propose the existence of DAGAT isoenzymes in the microsomal fraction. Furthermore, we unveiled an acyl-CoA-independent TAG synthase activity in lipid particles which is distinct from Dga1p and the phosphatidylcholine:DAGAT Lro1p. This acyl-CoA-independent TAG synthase utilizes DAG as an acceptor and free fatty acids as cosubstrates and occurs independently of the acyl-CoA synthases Faa1p to Faa4p. Based on lipid analysis of the respective deletion strains, Lro1p and Dga1p are the major contributors to total cellular TAG synthesis, whereas other TAG synthesizing systems appear to be of minor importance. In conclusion, at least three different pathways are involved in the formation of storage TAG in the yeast.
Identification and Assessment of Schlemm's Canal by Spectral-Domain Optical Coherence Tomography (2010)

Aqueous outflow structures, including SC and CCs, can be noninvasively assessed in the human eye. These measurements will be useful in physiological studies of AO and will be clinically useful in the determination of the impact of glaucoma therapies on IOP as well as presurgical planning.
Progressive perceptual audio rendering of complex scenes (2007)

Despite recent advances, including sound source clustering and perceptual auditory masking, high quality rendering of complex virtual scenes with thousands of sound sources remains a challenge. Two major bottlenecks appear as the scene complexity increases: the cost of clustering itself, and the cost of pre-mixing source signals within each cluster.
Photonic approach to making a material with a negative index of refraction (2003)

An approach to producing a composite material with negative refraction index is demonstrated. It is shown that a photonic structure consisting of two dielectric materials, with positive and negative dielectric permittivities, can support electromagnetic surface waves which exhibit the unusual electromagnetic property of left handedness (or negative refraction index). Depending on the dielectric materials, these surface waves localized at the dielectric interfaces can be either surface plasmons or phonons. The detailed geometry of the structure determines whether this composite left-handed material is isotropic or anisotropic.
High resolution chromatography : a practical approach (1999)

Separation by charge, size and hydrophobicity Practice and benefits of microcolumn HPLC Detection devices Capillary electrophoresis of peptides and proteins Lectins as affinity probes Nucleotide-and dye-ligand chromatography Synthetic peptides as affinity ligands Drugs and inhibitors as affinity ligands Immunoaffinity chromatography Strategies and methods for purification of Ca2+ -binding proteins Purification of DNA-binding proteins
Novel cross-talk within the IKK family controls innate immunity (2011)

Members of the IKK {IκB [inhibitor of NF-κB (nuclear factor κB)] kinase} family play a central role in innate immunity by inducing NF-κB- and IRF [IFN (interferon) regulatory factor]-dependent gene transcription programmes required for the production of pro-inflammatory cytokines and IFNs. However, the molecular mechanisms that activate these protein kinases and their complement of physiological substrates remain poorly defined. Using MRT67307, a novel inhibitor of IKKϵ/TBK1 (TANK {TRAF [TNF (tumour-necrosis-factor)-receptor-associated factor]-associated NF-κB activator}-binding kinase 1) and BI605906, a novel inhibitor of IKKβ, we demonstrate that two different signalling pathways participate in the activation of the IKK-related protein kinases by ligands that activate the IL-1 (interleukin-1), TLR (Toll-like receptor) 3 and TLR4 receptors. One signalling pathway is mediated by the canonical IKKs, which directly phosphorylate and activate IKKϵ and TBK1, whereas the second pathway appears to culminate in the autocatalytic activation of the IKK-related kinases. In contrast, the TNFα-induced activation of the IKK-related kinases is mediated solely by the canonical IKKs. In turn, the IKK-related kinases phosphorylate the catalytic subunits of the canonical IKKs and their regulatory subunit NEMO (NF-κB essential modulator), which is associated with reduced IKKα/β activity and NF-κB-dependent gene transcription. We also show that the canonical IKKs and the IKK-related kinases not only have unique physiological substrates, such as IκBα, p105, RelA (IKKα and IKKβ) and IRF3 (IKKϵ and TBK1), but also have several substrates in common, including the catalytic and regulatory (NEMO and TANK) subunits of the IKKs themselves. Taken together, our studies reveal that the canonical IKKs and the IKK-related kinases regulate each other by an intricate network involving phosphorylation of their catalytic and regulatory (NEMO and TANK) subunits to balance their activities during innate immunity.
Macaques primed with self-amplifying RNA vaccines expressing HIV-1 envelope and boosted with recombinant protein show potent T- and B-cell responses (2012)

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Microstructural Evolution of Melt Intercalated Polymer−Organically Modified Layered Silicates Nanocomposites (1996)

The microstructure of various (ordered and disordered) polymer−organically modified layered silicate hybrids, synthesized via static polymer melt intercalation, is examined with X-ray diffraction and transmission electron microscopy. The ordered intercalates exhibit microstructures very similar to the unintercalated organically modified layered silicate (OLS). Polymer intercalation occurs as a front which penetrates the primary OLS particle from the external edge. The disordered hybrids, on the other hand, exhibit heterogeneous microstructures with increased layer disorder and spacing toward the polymer−primary particle boundary. In these hybrids, individual silicate layers are observed near the edge, whereas small coherent layer packets separated by polymer-filled gaps are prevalent toward the interior of the primary particle. The heterogeneous microstructure indicates that the formation of these disordered hybrids occur by a more complex process than simple sequential separation of individual layers starting from the surface of the crystallites and primary particles. In general, the features of the local microstructure from TEM give useful detail to the overall picture that can be drawn from the XRD results and enhance the understanding of various thermodynamic and kinetic issues surrounding hybrid formation.