gebhart/patrepeval huggingface dataset:
Solid phase enzymatic glycerolysis of beef tallow resulting in a high yield of monoglyceride (1990)

A mixture of mono‐, di‐ and triglycerides was obtained when beef tallow was reacted with glycerol using lipase enzyme as a catalyst. The reaction was carried out batchwise in a small vessel with agitation by magnetic stirring. The yield of monoglyceride (MG) was greatly influenced by the reaction temperature—at higher temperatures (48–50°C) a yield of approximately 30% MG was obtained, while at lower temperatures (38–46°C) a yield of approximately 70% MG was obtained. A sharp transition was observed between the high and low yield equilibrium states. The temperature at which this transition occurred is called the critical temperature (T c ) and was found to be 46°C in the case of tallow. During the course of the reaction, when approximately 40% MG had been synthesized, the reaction mixture became solid but the reaction continued until approximately 70% MG had been synthesized. A yield of 70% MG also was obtained with tallow at 42°C when a glycerol/tallow mole ratio ranging from 1.5 to 2.5 was used. The free fatty acid content at equilibrium depended on the water concentration in the glycerol phase and varied from 0.5% to 11.0% when the water content ranged from 0.6% to 12.5%. Above 8% water content, the yield of MG was reduced. Of the commercially available lipases that were investigated, lipase from Pseudomonas fluorescens or Chromobacterium viscosum resulted in the highest yield of MG.
Postsynaptic Signaling and Plasticity Mechanisms (2002)

In excitatory synapses of the brain, specific receptors in the postsynaptic membrane lie ready to respond to the release of the neurotransmitter glutamate from the presynaptic terminal. Upon stimulation, these glutamate receptors activate multiple biochemical pathways that transduce signals into the postsynaptic neuron. Different kinds of synaptic activity elicit different patterns of postsynaptic signals that lead to short- or long-lasting strengthening or weakening of synaptic transmission. The complex molecular mechanisms that underlie postsynaptic signaling and plasticity are beginning to emerge.
Nitric oxide in inflammation and pain associated with osteoarthritis (2008)

Osteoarthritis (OA) is a degenerative disease involving chondrocytes, cartilage and other joint tissues, and has a number of underlying causes, including both biochemical and mechanical factors. Although proinflammatory factors including nitric oxide (NO) are associated with OA, there is recent evidence suggesting that NO and its redox derivatives may also play protective roles in the joint. However, the mechanisms that underlie the development and progression of OA are not completely understood. Experiments have demonstrated that NO plays a catabolic role in the development of OA and mediates the inflammatory response, is involved in the degradation of matrix metalloproteinases, inhibits the synthesis of both collagen and proteoglycans, and helps to mediate apoptosis. However, there is also evidence that in cultured chondrocytes the addition of exogenous NO may inhibit proinflammatory activation by preventing the nuclear localization of the transcription factor nuclear factor-kappaB, whereas the presence of peroxynitrite--a redox derivative of NO--appears to enhance the inflammatory response by sustaining the nuclear localization of nuclear factor-kappaB. In addition, under some conditions exogenous NO can stimulate collagen synthesis in cultured rat fibroblasts and human tendon cells. The protective roles of NO in multiple cell types, along with the opposing activities in cultured chondrocytes, suggest that NO may play additional protective roles in chondrocyte function. NO and its derivatives have a similarly complicated involvement in nociception and pain, which may contribute to the functional disability of OA. Further research may help to elucidate a potential role for NO-donating agents in the management of OA.
Use of Topical Coal Tar Foam for the Treatment of Psoriasis in Difficult-to-treat Areas. (2010)

Psoriasis in some body parts can be challenging to treat. Psoriasis in the scalp, palms and soles, and intertriginous areas differ from disease on other body parts, and treatment regimens must be tailored to these areas. The presence of hair, along with the frequent, thick scale of psoriasis makes the scalp a challenge to treat. Skin in the body's intertriginous areas is often thin, limiting treatment options for inverse psoriasis. Finally, palmoplantar psoriasis is often resistant to topical therapies because of the thickness of the skin. Moreover, cosmetic acceptibilty of a topical medicine is important when treating these areas. Coal tar has been used to treat psoriasis for hundreds of years. In a novel foam vehicle, coal tar 2% is versatile and may be used to treat psoriasis even in challenging areas of the body, such as the scalp, intertriginous areas, and palms and soles.
Augmented-Reality Visualization of Tissue Stiffness Data (2006)

In this article, we present an augmented-reality approach to visualization of human tissue stiffness data. Tissue stiffness values are measured by a special tactile sensor. A head-mounted display with a small video camera and a notebook computer are employed. We use two markers to create augmentation of a human organ by computer-generated visual information. One marker is attached to the patient’s body and is used to track the spacial position of the human organ where the measurements are done. Another marker is integrated into the tactile sensor. Tracking this marker helps to determine the shape of the human organ for subsequent data visualization. Results of stiffness measurements are depicted as semi-transparent three-dimensional objects superimposed on the patient’s body. We have experimented with sensing stiffness data and its visualization for a variety of objects, from a silicon breast model to animals organs. Experiments show that the developed augmented-reality system is useful in medical-physics measurements.
Suppressive Role of PPARγ-Regulated Endothelial Nitric Oxide Synthase in Adipocyte Lipolysis (2015)

eNOS-/- mice induce NASH development, and these findings provide new insights into the therapeutic approach for fatty liver disease and related disorders.
Utilization of waste gypsum in fertilizer production (2014)

Abstract The results of the study regarding utilization of the waste gypsum formed as a by-product during the production of the magnesium sulphate from dolomite have been presented. The use of this waste have been proposed for the production of granulated multi-component fertilizers containing 25% N; 8% S; 14% CaO and 3.5% MgO. In the process of fertilizer production, the urea which will be bounded with the calcium and magnesium sulphate from the waste in the form of adducts of CaSO4 . 4CO(NH2)2 and MgSO4 . CO(NH2)2 . 3H2O will be the source of nitrogen in the fertilizer. It has been stated on the basis of the laboratory tests that the fertilizer with the assumed composition can be obtained by means of granulation of the raw material pulp, along with the dried and ground product return. The possibility of the production of the fertilizer with good physical properties according to the assumed concept has been confirmed in the pilot scale
Tumor immunology and cancer immunotherapy: summary of the 2013 SITC primer (2014)

Knowledge of the basic mechanisms of the immune system as it relates to cancer has been increasing rapidly. These developments have accelerated the translation of these advancements into medical breakthroughs for many cancer patients. The immune system is designed to discriminate between self and non-self, and through genetic recombination there is virtually no limit to the number of antigens it can recognize. Thus, mutational events, translocations, and other genetic abnormalities within cancer cells may be distinguished as "altered-self" and these differences may play an important role in preventing the development or progression of cancer. However, tumors may utilize a variety of mechanisms to evade the immune system as well. Cancer biologists are aiming to both better understand the relationship between tumors and the normal immune system, and to look for ways to alter the playing field for cancer immunotherapy. Summarized in this review are discussions from the 2013 SITC Primer, which focused on reviewing current knowledge and future directions of research related to tumor immunology and cancer immunotherapy, including sessions on innate immunity, adaptive immunity, therapeutic approaches (dendritic cells, adoptive T cell therapy, anti-tumor antibodies, cancer vaccines, and immune checkpoint blockade), challenges to driving an anti-tumor immune response, monitoring immune responses, and the future of immunotherapy clinical trial design.
Efficacy of topical corticosteroid powder for nasal polyps: a double-blind, placebo-controlled study of budesonide (1995)

Budesonide has been used for a number of years as a topical nasal corticosteroid in the treatment of nasal allergy and nasal polyps. Recently, a new device for powder insufflation where no constituents or preservatives are included has been developed (Rhinocort Turbuhaler, Astra Draco AB, Sweden). The present investigation was designed in order to study the efficacy of topical budesonide powder as the only treatment of nasal polyps. A total of 126 patients entered the study. The medical history and clinical recordings included symptoms and signs, a semiquantitative test of smell and measurement of nasal expiratory peak flow index. Medication was either 200 or 400 micrograms of budesonide powder b.i.d. or placebo. After 1 month an overall assessment of treatment efficacy was made to determine whether the treatment had been a success or a failure. The results showed a statistically significant improvement of symptoms and signs in the actively treated groups. The increase in expiratory peak flow index was about 60% in the actively treated groups as opposed to 16% in the placebo group. The overall assessment of treatment efficacy showed success in about 82% of actively treated patients as opposed to about 43% in the placebo group. It is concluded that budesonide powder is useful in the treatment of nasal polyps.
Synthesis and biologic evaluation of 64Cu-labeled rhenium-cyclized alpha-MSH peptide analog using a cross-bridged cyclam chelator. (2007)

Our data suggest the superior stability of the (64)Cu-CBTE2A moiety compared with (64)Cu-DOTA, making (64)Cu-CBTE2A-ReCCMSH(Arg(11)) an ideal candidate for the PET of malignant melanoma.
Cathepsin B inhibition prevents trypsinogen activation and reduces pancreatitis severity (2002)

Intrapancreatic activation of trypsinogen is believed to play a critical role in the initiation of acute pancreatitis, but mechanisms responsible for intrapancreatic trypsinogen activation during pancreatitis have not been clearly defined. In previous in vitro studies, we have shown that intra-acinar cell activation of trypsinogen and acinar cell injury in response to supramaximal secretagogue stimulation could be prevented by the cell permeant cathepsin B inhibitor E64d (Saluja A, Donovan EA, Yamanaka K, Yamaguchi Y, Hofbauer B, and Steer ML. Gastroenterology 113: 304-310, 1997). The present studies evaluated the role of intrapancreatic trypsinogen activation, this time under in vivo conditions, in two models of pancreatitis by using another highly soluble cell permeant cathepsin B inhibitor, L-3-trans-(propylcarbamoyl)oxirane-2-carbonyl-L-isoleucyl-L-proline methyl ester (CA-074me). Intravenous administration of CA-074me (10 mg/kg) before induction of either secretagogue-elicited pancreatitis in mice or duct infusion-elicited pancreatitis in rats markedly reduced the extent of intrapancreatic trypsinogen activation and substantially reduced the severity of both pancreatitis models. These observations support the hypothesis that, during the early stages of pancreatitis, trypsinogen activation in the pancreas is mediated by the lysosomal enzyme cathepsin B. Our findings also suggest that pharmacological interventions that inhibit cathepsin B may prove useful in preventing acute pancreatitis or reducing its severity.
Topoisomerase I inhibitors for the treatment of brain tumors (2008)

Patients with primary malignant brain tumors have a poor prognosis. Standard treatment includes surgical resection, radiation therapy and chemotherapy. Topoisomerase I inhibitors such as topotecan and irinotecan (CPT-11) represent one class of chemotherapy drugs that have been used in this disease. Recent clinical trials have shown major antitumor activity in recurrent glioblastoma when adding the antiangiogenesis drug bevacizumab with CPT-11. The combination of targeted agents to topoisomerase I inhibitors represent a novel and promising approach. This review will summarize clinical trials with topoisomerase I inhibitors and discuss new treatment strategies for primary malignant brain tumors.
In Vivo Recellularization of Plain Decellularized Xenografts With Specific Cell Characterization in the Systemic Circulation: Histological and Immunohistochemical Study (2006)

Decellularized biological scaffolds have been used for the tissue engineering of heart valves with good results in the pulmonary circulation. However, little information is available on the recellularization of plain decellularized biological scaffolds in the systemic circulation. The aim of this study was to determine whether plain decellularized xenografts (PDXs) can recellularize with specific cell characterization in the systemic circulation. The xenogenic aortic valved conduit grafts of rabbits were implanted in the abdominal aorta of dogs after decellularization. The grafts were explanted at 4, 12, or 24 weeks after implantation for histological, immunohistochemical examination, scanning electron microscope, and Western blot analysis. Although the valvular structures were completely lost after implantation, supravalvular conduits showed normal shapes and endothelialization. The PDXs were repopulated with basic vascular cell components in approximate natural proportions without immunological responses. The PDXs had been recellularized with specific cell characterization in the systemic circulation.
Cathepsin A Is Expressed in a Cell- and Region-specific Manner in the Testis and Epididymis and Is Not Regulated by Testicular or Pituitary Factors (2000)

The epithelial cells of the testis are involved in the production, differentiation, and sustenance of sperm, and those of the epididymis play a major role in sperm maturation, protection, and storage. These tissues express various proteins that respond differently to androgens. Cathepsin A is a multifunctional lysosomal carboxypeptidase that also functions as a protective and an activator protein for neuraminidase and beta-galactosidase. In this study, cathepsin A was immunolocalized by light and electron microscopy using a polyclonal affinity-purified antibody on the testis and epididymis of normal, orchidectomized with or without testosterone supplementation, efferent duct-ligated, and hypophysectomized adult rats. In normal rats, cathepsin A expression was noted in lysosomes of Sertoli and Leydig cells but not in germ cells of the testis, as well as nonciliated cells of the efferent ducts. In the epididymis, a cell- and region-specific distribution of cathepsin A was noted. In experimentally treated animals, no changes were noted in the expression of cathepsin A. Immunolabeling of tissues examined at the electron microscopic level revealed that lysosomes were reactive. These data indicate cell- and region-specific expression of cathepsin A in cells of the testis and epididymis and also indicate that cathepsin A expression is not regulated by testicular or pituitary factors.
Instance-Aware Semantic Segmentation via Multi-task Network Cascades (2016)

Semantic segmentation research has recently witnessed rapid progress, but many leading methods are unable to identify object instances. In this paper, we present Multitask Network Cascades for instance-aware semantic segmentation. Our model consists of three networks, respectively differentiating instances, estimating masks, and categorizing objects. These networks form a cascaded structure, and are designed to share their convolutional features. We develop an algorithm for the nontrivial end-to-end training of this causal, cascaded structure. Our solution is a clean, single-step training framework and can be generalized to cascades that have more stages. We demonstrate state-of-the-art instance-aware semantic segmentation accuracy on PASCAL VOC. Meanwhile, our method takes only 360ms testing an image using VGG-16, which is two orders of magnitude faster than previous systems for this challenging problem. As a by product, our method also achieves compelling object detection results which surpass the competitive Fast/Faster R-CNN systems. The method described in this paper is the foundation of our submissions to the MS COCO 2015 segmentation competition, where we won the 1st place.
The Fate of Inhaled Nitric Oxide (1983)

Conference Abstract| September 01 1983 The Fate of Inhaled Nitric Oxide C. Borland; C. Borland 1Department of Respiratory Physiology, Addenbrooke's Hospital, Cambridge Search for other works by this author on: This Site PubMed Google Scholar A Chamberlain; A Chamberlain 1Department of Respiratory Physiology, Addenbrooke's Hospital, Cambridge Search for other works by this author on: This Site PubMed Google Scholar T. Higenbottam T. Higenbottam 1Department of Respiratory Physiology, Addenbrooke's Hospital, Cambridge Search for other works by this author on: This Site PubMed Google Scholar Clin Sci (Lond) (1983) 65 (3): 37P. https://doi.org/10.1042/cs065037P Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation C. Borland, A Chamberlain, T. Higenbottam; The Fate of Inhaled Nitric Oxide. Clin Sci (Lond) 1 September 1983; 65 (3): 37P. doi: https://doi.org/10.1042/cs065037P Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. © 1983 The Biochemical Society and the Medical Research Society1983 Article PDF first page preview Close Modal You do not currently have access to this content.
Cortisol in Saliva - Reference Ranges and Relation to Cortisol in Serum (1995)

The aim of this study was to establish morning and evening reference ranges for cortisol in saliva. Another objective was to compare the concentrations of the mainly free cortisol in saliva to those of total cortisol in serum as determined with a commercial radioimmunoassay. The concentrations were determined in matched samples of saliva and serum collected at 8 am and 10 pm from 197 healthy volunteers. The saliva samples were stable for at least 7 days at room temperature and for 9 months at -20 degrees C. Reference ranges, the central 95%, were estimated to 3.5-27.0 nmol/l at 8 am and < 6.0 nmol/l at 10 pm. The intra-assay coefficient of variation (CV) was below 5% and total CV below 10%. The relation between the cortisol concentrations in serum and saliva was non-linear with r = 0.86 for serum concentrations < 450 nmol/l and r = 0.44 for serum concentrations > or = 450 nmol/l. In conclusion, the satisfactory precision of the analysis and the simple non-invasive sampling procedure suggest that saliva may be used for cortisol measurements in situations where blood sampling is difficult to perform.
Pharmacological Regulation of Dyslipoproteinaemia in Insulin Resistant States (2008)

Dyslipidaemia is a common and consistent abnormality in insulin resistant subjects with obesity and type 2 diabetes mellitus associated with increased risk of cardiovascular disease. Lipoprotein metabolism is complex and abnormal plasma concentrations can result from alterations in the rates of production and/or catabolism of diverse lipoprotein particles. Our understandings of the dysregulation and therapeutic regulation of lipoprotein transport in insulin resistant states has relied on the application of advances in stable isotope and modelling methods. Dysregulation of lipoprotein metabolism in these circumstances may be caused by a combination of overproduction of VLDL apolipoprotein (apoB) B-100 and VLDL-apoC-III, decreased catabolism of apoB-containing particles, and increased catabolism of HDL apoA-I particles. These abnormalities may be consequent on a global metabolic effect of insulin resistance and accumulation of visceral fat. Several pharmacological treatments, such as statins, fibrates or fish oil can correct the dyslipidaemia by diverse kinetic mechanisms of action, including decreased secretion of apoB and apoC-III, and increased catabolism of apoB, as well as increased secretion and decreased catabolism of apoA-I. Newer agents, including insulin sensitizers, cholesterol absorption inhibitors, CETP inhibitors, peroxisome proliferator-activated receptor-delta agonists and endocannabinoid-1 receptor blockers, have also been shown to improve plasma lipid and lipoprotein abnormalities in insulin resistant states; their mechanisms of action are at present being investigated. Rimonabant is the endocannabinoid receptor blocker shown to decrease cardiometabolic risk in insulin resistant subjects. The complementary mechanisms of action of different agents support the use of combination regimens in treating dyslipoproteinaemia in subjects with central obesity and type 2 diabetes.
Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli. (1984)

We have identified hybrid plasmids carrying the melibiose operon of Escherichia coli in a colony bank of Clarke and Carbon (Tsuchiya, T., Ottina, K., Moriyama, Y., Newman, M., and Wilson, T. H. (1982) J. Biol. Chem. 257, 5125-5128). Using one of the plasmids as a starting material, the DNA fragments containing the melibiose operon were recloned in a vector pBR322. Restriction maps were prepared, and several DNA segments were subcloned into pBR322. Genetic complementation tests and recombination analyses using those plasmids and melA- and melB- mutants as well as biochemical analyses of mel mutants transformed with those plasmids enabled us to determine the physical location of promoter, melA, and melB on the DNA segment. The size of the melAB region was about 3,000 base pairs. Gene products were identified using maxicells harboring plasmids carrying the melibiose operon. The apparent molecular weight of the alpha-galactosidase (coded by melA) was about 50,000 and that of the melibiose carrier (coded by melB) was about 31,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The melibiose carrier was also identified as a 30,000-dalton protein in reconstituted proteoliposomes which possessed melibiose transport activity.