gebhart/patrepeval huggingface dataset:
Internally calibrated quantification of protein analytes in human serum by fluorescence immunoassays in disposable elastomeric microfluidic devices (2008)

Herein we report on reliable reproducible quantification of protein analytes in human serum by fluorescence sandwich immunoassays in disposable PDMS microfluidic chips. The system requires 1,000 times less sample than typical clinical blood tests and is specifically shown to measure ferritin down to 250 pM in human serum. The in-built calibration method of spiking the serum with known concentrations of commercially available antigen avoids common sources of error and improves the reliability of the test results. The reported microfluidic system is an important new tool for fundamental scientific research, offering sensitive immunoassay measurements in small but complex biosamples. The system is also a further step towards comprehensive affordable "point-of-care" biomedical diagnostics.
Bacteriostats. VI.1a Bacteriostatic Activities of Some Substituted Guanidines1b (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBacteriostats. VI.1a Bacteriostatic Activities of Some Substituted Guanidines1bA. F. McKay, D. L. Garmaise, H. A. Baker, L. R. Hawkins, V. Falta, R. Gaudry, and G. Y. ParisCite this: J. Med. Chem. 1963, 6, 5, 587–595Publication Date (Print):September 1, 1963Publication History Published online1 May 2002Published inissue 1 September 1963https://pubs.acs.org/doi/10.1021/jm00341a028https://doi.org/10.1021/jm00341a028research-articleACS PublicationsRequest reuse permissionsArticle Views294Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Methods to Identify and Compare Parenteral Nutrition Administered From Hospital‐Compounded and Premixed Multichamber Bags in a Retrospective Hospital Claims Database (2011)

Exploratory analysis suggests that comparisons of PN administered via compounding and MCB are possible using the Premier data. The ability to control for many identifiable risk factors allows data to be presented for the use of PN and related outcomes in both a clinically sensible and relevant manner, albeit with some limitations.
Postoperative Bacterial/Fungal Infections: A Challenging Problem in Critically Ill Patients after Abdominal Surgery (2007)

Critically ill patients after extended surgical procedures are at high risk for postoperative infections. The overall incidence of sepsis increased constantly over the last decade, whereas sepsis-related mortality decreased, due to new intensive care options. After extended intra-abdominal surgery the abdomen is the predominant focus of sepsis, followed by respiratory tract infections. Unspecific clinical signs lead to the diagnosis of postoperative sepsis. Early focus identification by clinical, laboratory and radiologic examination is of major importance for a promising therapy, followed by immediately initiated procedures for source control and an adequate anti-infective drug therapy. For nosocomial sepsis, the use of antimicrobial regimens with extended spectra and potent activity against both Gram-negative and Gram-positive bacterial pathogens are warranted. Fungal infections play a great role for immunocompromized patients, e.g. after solid organ transplantation or under chronic corticoid therapy. An increasing incidence of multi-drug resistance in bacterial and fungal isolates has been observed in the last years. This is becoming a growing problem also for critically ill patients after abdominal surgery. Standardized treatment protocols including supportive and adjunctive therapy and the use of modern anti-infective agents may lead to a decrease in postoperative mortality due to sepsis.
Effect of vitamin E on plasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures during wound healing in normal and diabetic rats (2002)

Vitamin E is composed of various subfamilies that include tocopherols and tocotrienols. These compounds have antioxidant properties but differ in structure, dietary source and potency. In this study we evaluated the efficacy of alpha-tocopherol as an antioxidant and its role in wound closure in normal and streptozotocin-induced diabetic rats. The healing of 6 cm linear incisions created on the back of each male Sprague-Dawley rat (250-300 g) was monitored by measuring the length of the wounds daily. The rats were divided into two categories; normal and streptozotocin-induced diabetic rats. For each category, the animals were further divided into two groups; those untreated and those receiving 200 mg/kg bodyweight alpha-tocopherols daily by oral gavage. All rats were fed standard food and water ad libitum. Blood samples were taken at 0, 5 and 10 days after the wounds were created for the determination of malondialdehyde levels and red cell superoxide dismutase, catalase and glutathione peroxidase activities. The results showed that alpha-tocopherol reduced plasma malondialdehyde levels, increased glutathione peroxidase activity and accelerated the rate of wound closure in treated rats.
Rapamycin blocks tumor progression: unlinking immunosuppression from antitumor efficacy1 (2002)

Our findings, in addition to unlinking mechanisms of immunosuppression from that of tumor progression, suggest that rapamycin may be of value for the management of posttransplant malignancy.
Evaluation of Immune Globulin and Recombinant Interferon‐α2b for Treatment of Experimental Ebola Virus Infections (1999)

A passive immunization strategy for treating Ebola virus infections was evaluated using BALB/ c mice, strain 13 guinea pigs, and cynomolgus monkeys. Guinea pigs were completely protected by injection of hyperimmune equine IgG when treatment was initiated early but not after viremia had developed. In contrast, mice were incompletely protected even when treatment was initiated on day 0, the day of virus inoculation. In monkeys treated with one dose of IgG on day 0, onset of illness and viremia was delayed, but all treated animals died. A second dose of IgG on day 5 had no additional beneficial effect. Pretreatment of monkeys delayed onset of viremia and delayed death several additional days. Interferon-alpha2b (2 x 10(7) IU/kg/day) had a similar effect in monkeys, delaying viremia and death by only several days. Effective treatment of Ebola infections may require a combination of drugs that inhibit viral replication in monocyte/macrophage-like cells while reversing the pathologic effects (e.g., coagulopathy) consequent to this replication.
High-Resolution Structure of the Oligomerization Domain of p53 by Multidimensional NMR (1994)

The three-dimensional structure of the oligomerization domain (residues 319 to 360) of the tumor suppressor p53 has been solved by multidimensional heteronuclear magnetic resonance (NMR) spectroscopy. The domain forms a 20-kilodalton symmetric tetramer with a topology made up from a dimer of dimers. The two primary dimers each comprise two antiparallel helices linked by an antiparallel beta sheet. One beta strand and one helix are contributed from each monomer. The interface between the two dimers forming the tetramer is mediated solely by helix-helix contacts. The overall result is a symmetric, four-helix bundle with adjacent helices oriented antiparallel to each other and with the two antiparallel beta sheets located on opposing faces of the molecule. The tetramer is stabilized not only by hydrophobic interactions within the protein core but also by a number of electrostatic interactions. The implications of the structure of the tetramer for the biological function of p53 are discussed.
Thermochemical biofuel production in hydrothermal media: A review of sub- and supercritical water technologies (2008)

Hydrothermal technologies are broadly defined as chemical and physical transformations in high-temperature (200–600 °C), high-pressure (5–40 MPa) liquid or supercritical water. This thermochemical means of reforming biomass may have energetic advantages, since, when water is heated at high pressures a phase change to steam is avoided which avoids large enthalpic energy penalties. Biological chemicals undergo a range of reactions, including dehydration and decarboxylation reactions, which are influenced by the temperature, pressure, concentration, and presence of homogeneous or heterogeneous catalysts. Several biomass hydrothermal conversion processes are in development or demonstration. Liquefaction processes are generally lower temperature (200–400 °C) reactions which produce liquid products, often called “bio-oil” or “bio-crude”. Gasification processes generally take place at higher temperatures (400–700 °C) and can produce methane or hydrogen gases in high yields.
On the synthesis and animation of 5‐chloro‐and 5‐bromo‐1,7‐naphthyridine (1978)

Abstract A new synthesis of 5‐chloro‐ and 5‐bromo‐1,7‐naphthyridine, using 8‐amino‐1,7‐naphthyridine as starting material is described. On amination with potassium amide in liquid ammonia, the 5‐bromo compound undergoes a tele‐amination into 8‐amino‐ and 2‐amino‐1,7‐naphthyridine and a Chichibabin reaction yielding 8‐amino‐5‐bromo‐1,7‐naphthyridine. The reaction with the 5‐chloro compound occurs at a much lower rate than the 5‐bromo compound and only gives 8‐amino‐5‐chloro‐1,7‐naphthyridine in a small yield. Convincing 1 H‐nmr evidence is presented, showing that the 5‐bromo‐ and 5‐chloro‐1,7‐naphthyridine give addition of the amide ion at position 8 and that the 5‐chloro compound also gives addition at position 2.
Error control coding : fundamentals and applications (1983)

1. Coding for Reliable Digital Transmission and Storage. 2. Introduction to Algebra. 3. Linear Block Codes. 4. Important Linear Block Codes. 5. Cyclic Codes. 6. Binary BCH Codes. 7. Nonbinary BCH Codes, Reed-Solomon Codes, and Decoding Algorithms. 8. Majority-Logic Decodable Codes. 9. Trellises for Linear Block Codes. 10. Reliability-Based Soft-Decision Decoding Algorithms for Linear Block Codes. 11. Convolutional Codes. 12. Trellis-Based Decoding Algorithms for Convolutional Codes. 13. Sequential and Threshold Decoding of Convolutional Codes. 14. Trellis-Based Soft-Decision Algorithms for Linear Block Codes. 15. Concatenated Coding, Code Decomposition ad Multistage Decoding. 16. Turbo Coding. 17. Low Density Parity Check Codes. 18. Trellis Coded Modulation. 19. Block Coded Modulation. 20. Burst-Error-Correcting Codes. 21. Automatic-Repeat-Request Strategies.
Animal Models for Acquired Immunodeficiency Syndrome (1987)

Substantial advances have already been made in the understanding of acquired immunodeficiency syndrome (AIDS). The major issues for AIDS research during the next few years must be practical ones: the development of a safe, effective vaccine for individuals not yet infected with the causative virus and the development of drug therapies for those already infected. Suitable animal models will be needed for studies designed to achieve these goals. Areas of investigation in animal models can be divided into four categories on the basis of increasing direct relevance to AIDS in humans: retroviruses that have no obvious, close relation to human immunodeficiency virus (HIV) but can induce chronic diseases with manifestations that include immunologic abnormalities; ungulate lentiviruses; HIV-related viruses of Old World primates; and HIV infection of chimpanzees. It is hoped that important research developments in experimental models can be quickly extrapolated to human AIDS.
The Maillard Reaction in Foods and Nutrition (1983)

A historical review with107 references. Life and work of Louis-Camille Maillard (Feb. 4, 1878 - May 12, 1936) are described. The first use of the index term Maillard reaction in Chemical Abstracts was in 1950. German scientists with early interest in this reaction were Lintner (1912) and Ruckdeschel (1914). Several aspects of this reaction are reviewed with emphasis on the work of Japanese scientists.
Transendothelial migration of neutrophils involves integrin-associated protein (CD47). (1995)

Inflammation is a primary pathological process. The development of an inflammatory reaction involves the movement of white blood cells through the endothelial lining of blood vessels into tissues. This process of transendothelial cell migration of neutrophils has been shown to involve neutrophil beta 2 integrins (CD18) and endothelial cell platelet-endothelium cell adhesion molecules (PECAM-1; CD31). We now show that F(ab')2 fragments of the monoclonal antibody B6H12 against integrin-associated protein (IAP) blocks the transendothelial migration of neutrophils stimulated by an exogenous gradient of the chemokine interleukin 8 (IL-8; 60% inhibition), by the chemotactic peptide N-formyl-methionylleucylphenylalanine (FMLP; 76% inhibition), or by the activation of the endothelium by the cytokine tumor necrosis factor alpha (98% inhibition). The antibody has two mechanisms of action: on neutrophils it prevents the chemotactic response to IL-8 and FMLP, and on endothelium it prevents an unknown but IL-8-independent process. Blocking antibodies to IAP do not alter the expression of adhesion proteins or production of IL-8 by endothelial cells, and thus the inhibition of neutrophil transendothelial migration is selective. These data implicate IAP as the third molecule essential for neutrophil migration through endothelium into sites of inflammation.
Filapodial Sensing of Nanotopography in Osteoprogenitor Cells (2005)

INTRODUCTION: Micro- and nanotopography can have been shown to be able to change cell adhesion, morphology, cytoskeleton, proliferation and differentiation. It is proposed that filopodia presented at the cells leading edge are able to gather special information and thus ‘sense’ nanotopography around cells. Here, 120 nm diameter pits in both hexagonal and orthogonal arrangement were used to allow observation of filopodia production and interaction from primary human mesenchymal bone marrow stromal cells (HMSC). METHODS: Electron beam lithography was used to fabricate arrays of pits with diameters of 120 nm and centre to centre spacing of 300 nm in either orthogonal or hexagonal arrangement in Si. Using sputter coating and electroplating, Ni intermediaries were made from which replicas of the Si masters were produced in polymethylmethacrylate (PMMA). HMSC’s at passage 1 were cultured on the structures for 4 days at an intial density of 1 x 10 4 /ml in alpha-MEM media with 10% FCS. Half the samples were then fixed with 1.5% gluteraldehyde and prepared for SEM analysis using standard methods. A Hitachi 800 scanning electron microscope running at 5kV was used to observe filopodia. The other half of the samples were fixed in 4% formaldehyde and Coomassie blue stained. Image J was then used to calculate the perimeters of cells in pixels. Students t-test (* = p<0.05) was used to test significance. RESULTS: Filopodia were observed from HBMCs on each material (fig 1). Following normalizion of the results with cell perimeter, it was observed that cells on nanotopography produce significantly more filopodia compared to those on planar control (fig 2). Filopodial interaction with pits (filopodia deemed to be located at the edge of a pit or in a pit) on the orthogonal and hexagonal structures was detected at 67 and 66 % respectively. This incidence is highly significant given the pits cover 14% of the material surface area when calculating for just the pits or 27% if also including the lips produced by imprinting. Fig 1: HMSC filopodia on (A) flat control PMMA, (B) orthogonal nanopits and (C) hexagonal nanopits.
Surface Chemistry Studies of Copper Chemical Mechanical Planarization (2001)

Surface chemistry studies of the chemical mechanical planarization (CMP) of copper are presented in this paper. Blanket copper samples were polished with an acidic alumina-based slurry which contains an organic acid salt (phthalic acid salt) and an oxidizer Surface studies using X-ray photoelectron spectroscopy (XPS) were performed on copper samples after chemical etching or CMP in order to determine the effect that different polishing parameters (i.e., pH and oxidizer concentration) have on the copper surface. XPS studies were also done on samples that were passively soaked in an acidic slurry mixture containing different concentrations of to determine how the chemical action alone affects the removal of copper. The etching results revealed that a cuprous oxide forms on the surface of etched metal while polished samples showed CuO and The effect of these copper oxide films on the removal of copper in passive etching and chemical mechanical polishing is discussed. © 2001 The Electrochemical Society. All rights reserved.
Is 60 Days of Ciprofloxacin Administration Necessary for Postexposure Prophylaxis for Bacillus anthracis ? (2008)

Sixty days of ciprofloxacin administration at 500 mg every 12 h is currently recommended for the prophylaxis of inhalational exposure to Bacillus anthracis. We examined Bacillus anthracis (Delta-Sterne strain) in our hollow-fiber infection model. We measured the ciprofloxacin concentrations achieved and the number of organisms present before heat shock (total population) and after heat shock (spore population). We fit a mathematical model to these data. Monte Carlo simulation with differing initial spore burdens (3, 5, and 6.9 log(10) CFU/ml) demonstrated that 35 days of this regimen would completely clear the spore burden in 95% of patients. Durations of 110 days did not achieve 99.9% eradication, irrespective of initial burden, because of between-patient variance in drug pharmacokinetics. Given the absence of person-to-person transmission for Bacillus anthracis, adverse drug effects with long-term ciprofloxacin administration, and the possibility of engendering resistance in bodily flora, shorter prophylaxis duration should be given consideration, along with careful monitoring of all exposed individuals.
Interaction between deletion delta-thalassemia and beta zero-thalassemia (codon 39 nonsense mutation) in a Sardinian family. (1989)

This paper describes individuals of Sardinian descent with heterozygous normal-Hb A2 beta-thalassemia who have a chromosome with a 7.2 Kb deletion in the psi beta/delta globin intergenic region and a chromosome with the codon 39 nonsense mutation. The propositus has the typical thalassemia-like red cell indices with a relatively high Hb level (13.4 g/dl) for a beta-thalassemia carrier, indicating a normal function of the beta-globin gene in cis to the 7.2 Kb deletion. The deletion starts between the psi beta and delta genes and ends in the IVS-2 of the delta-gene. His breakpoints are identical to a previously described deletion detected in the homozygous state in a patient with thalassemia intermedia, homozygous for G-greater than A mutation at position 5 of IVS-1. Because the mutation herein described silences the delta-globin gene and leaves intact the function of the in cis beta-globin gene we propose the definition of deletion delta zero-thalassemia.
Homozygous familial amyloidosis, Finnish type: demonstration of glomerular gelsolin-derived amyloid and non-amyloid tubular gelsolin. (1993)

The immunohistochemical findings in the kidneys of two sisters with homozygous familial amyloidosis of Finnish type, an autosomal dominant disorder of gelsolin catabolism, is described. The disease, manifesting with nephrotic syndrome and end-stage renal failure was characterized by heavy glomerular deposits of gelsolin-derived amyloid. Immunostaining also revealed the presence of gelsolin in the tubular epithelium that was Congo-red negative. It is concluded that homozygous Finnish amyloidosis leads to a severe nephropathy due to the deposition of fragments of mutant gelsolin.
Low noise microwave parametric amplifier (1985)

We report the lowest noise temperature of any microwave amplifier except the maser. This amplifier is a nearly degenerate superconducting parametric amplifier at X-band consisting of a low β, thin film, single junction SQUID, a monolithic 50 Ω to 1 Ω impedance matching network, and a cooled circulator. Nb/Nb <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> O <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</inf> /Pb alloy junctions were incorporated into Nb and Pb-in circuitry on 1×2 cm silicon chips. The SQUID was phase-biased near 90° and driven by an external pump at approximately twice the operating frequency. The self-resonance frequency of the SQUID inductance and junction capacitance was set at the operating frequency of 8.2GHz. The operating charac teristics depended on the pump amplitude, the junction phase bias, and the SQUID parameters. For small values of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\beta &lt; 1</tex> , the maximum gain in both the signal and idler channels was ≈10dB, the bandwidth = 170MHz, and the single-sidehand noise temperature was 6K with an uncertainty of (+15 to -7)K. For higher β, the gain increased to nearly 30dB with increased noise and parametric oscillations occurring near the pump subharmonic frequency.
Cable positioning using compasses, tailbuoys, and acoustic devices (1991)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 1991Cable positioning using compasses, tailbuoys, and acoustic devicesAuthors: M. H. HoustonM. H. HoustonWestern Atlas Internationalhttps://doi.org/10.1190/1.1888904 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1888904FiguresReferencesRelatedDetailsCited ByDevelopment of an Electro Impedance Tomography-based Platform for Measurement of burial Depth of Cables in Subsea Sediments SEG Technical Program Expanded Abstracts 1991ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 1991 Pages: 1646 publication data© 1991 Copyright © 1991 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished: 10 Feb 2005 CITATION INFORMATION M. H. Houston, (1991), "Cable positioning using compasses, tailbuoys, and acoustic devices," SEG Technical Program Expanded Abstracts : 761-763. https://doi.org/10.1190/1.1888904 Plain-Language Summary PDF DownloadLoading ...
About Supramolecular Assemblies of π-Conjugated Systems (2005)

No abstract