gebhart/patrepeval huggingface dataset:
Effective Area Limit for Large Mode Area Laser Fibers (2008)

We analyze practical constraints of fibers designs for achieving large mode area and single mode operation for fiber lasers, and provide estimated upper limits for the core diameter and effective area.
Transcription Regulation by Initiating NTP Concentration: rRNA Synthesis in Bacteria (1997)

The sequence of a promoter determines not only the efficiency with which it forms a complex with RNA polymerase, but also the concentration of nucleoside triphosphate (NTP) required for initiating transcription. Escherichia coli ribosomal RNA (rrn P1) promoters require high initiating NTP concentrations for efficient transcription because they form unusually short-lived complexes with RNA polymerase; high initiating NTP concentrations [adenosine or guanosine triphosphate (ATP or GTP), depending on the rrn P1 promoter] are needed to bind to and stabilize the open complex. ATP and GTP concentrations, and therefore rrn P1 promoter activity, increase with growth rate. Because ribosomal RNA transcription determines the rate of ribosome synthesis, the control of ribosomal RNA transcription by NTP concentration provides a molecular explanation for the growth rate-dependent control and homeostatic regulation of ribosome synthesis.
Purification and Characterization of a Marine Bacterial -Galactoside 2,6-Sialyltransferase from Photobacterium damsela JTO16O (1996)

A bacterial sialyltransferase, named sialyltransferase 0160, was purified from a marine bacterium that had been isolated from seawater from Sagami Bay, Kanagawa. This strain has been identified as Photobacterium damsela, and named P. damsela JT0160. Sialyltransferase 0160 was purified 688-fold to homogeneity from the crude extract of the cells with a yield of 19% using a combination of anion exchange chromatography, hydroxyapatite chromatography, gel filtration chromatography, and affinity chromatography. The purified enzyme migrated as a single band (61 kDa) on sodium dodecyl sulfate-polyacrylamide gel. This sialyltransferase was found to be a beta-galactoside alpha 2,6-sialyltransferase [EC 2.4.99.1] which catalyzes the incorporation of NeuAc from CMP-NeuAc into the galactose residue of the carbohydrate chain at position 6 on the basis of an analysis of the enzymatic reaction products with HPLC, 1H-, 13C-NMR spectroscopy, and fast atom bombardment mass spectroscopy.
Shape and Reflectance from Image Intensities (2005)

This paper presents new results in the area of image intensity understanding. We address the problem of recovering three-dimensional shapes of surfaces from twc-dimensional intensity images. Previous shape-fromintensity methods are not applicable to surfaces with varying reflectance properties. These methods are also inaccurate in the case of concave surfaces as they do not account for interreflections. Here, we present shape recovery methods that overcome both these problems. Our solutions are based on physics-based models of reflection and interreflection. Multiple images of a surface are obtained by varying the source direction. Shape and reflectance parameters are computed simultaneously from the measured image intensities. Experimental results are included to demonstrate the accuracy and robustness of the proposed shape recovery methods.
Acidic and basic FGFs dilate arterioles of skeletal muscle through a NO-dependent mechanism (1996)

Fibroblast growth factors (FGFs) have been known to be potent stimulators of vascular endothelial cell proliferation and angiogenesis. Recent experimental evidence indicates that basic FGF (bFGF) is also involved in modulation of arterial pressure. In this study, we investigated the effects of acidic FGF (aFGF) and bFGF on muscle microcirculation using isolated arterioles and intact cremaster muscles of the at. In isolated microvessels, aFGF and bFGF (10(-12)-10(-8) M) significantly increased arteriolar diameter in a dose-dependent and time-dependent manner. This effect was abolished during inhibition of nitric oxide synthesis by NG-monomethyl-L-arginine (L-NMMA, 10(-4) M) but was not affected by indomethacin (10(-4) M), an inhibitor of the cyclooxygenase pathway of arachidonic acid metabolism. The vasodilation induced by FGFs was not observed in endothelium-denuded vessels. Furthermore, we studied microvascular hemodynamics in response to the growth factors in the cremaster muscle using intravital microscopy. Both aFGF and bFGF dilated arterioles of the intact cremaster muscle in a pattern similar to that observed in the isolated arterioles. At a concentration of 10(-10) M, aFGF caused a 19% increase in vessel diameter and 56% increase in blood flow. Administration of L-NMMA blocked by FGF-induced vasodilation and hyperemia. These results suggest that FGFs modulate blood flow in the skeletal muscle by acting on the endothelium of arterioles. The signaling mechanism of FGF-induced vasodilation involves the synthesis of nitric oxide by arteriolar endothelium.
Using LabVIEW to measure transformer residual flux for inrush current reduction (2009)

Power transformers can experience large inrush currents upon energization, the severity of which depends on the source strength, the angle of the applied voltage at energization, and the residual flux of the transformer. A novel inrush current reduction strategy has been investigated which involves setting the transformer's residual flux. Testing of this strategy required a measurement system capable of measuring transformer residual flux. This paper details the construction of the flux measurement system, based on LabVIEW software, and presents test results which confirmed properly measured residual flux values.
Congenital disorders of glycosylation. Part II. Defects of protein O-glycosylation. (2013)

Glycosylation is a form of post-translational modification of proteins and occurs in every living cell. The carbohydrate chains attached to the proteins serve various functions. There are two main types of protein glycosylation: N-glycosylation and O-glycosylation. In this paper, we describe the O-glycosylation process and currently known congenital disorders of glycosylation associated with defects of protein O-glycosylation. This process takes place in the cis Golgi apparatus after N-glycosylation and folding of the proteins. The O-glycosylation is essential in the biosynthesis of mucins, the formation of proteoglycan core proteins and blood group proteins. Most common forms of O-glycans are the mucin-type glycans. There are more than 20 known disorders related to O-glycosylation disturbances. We review 8 of the following diseases linked to defects in the synthesis of O-xylosylglycans, O-N acetylgalactosaminylglycans, O-xylosyl/N-acetylglycans, O-mannosylglycans, and O-fucosylglycans: multiple exostoses, progeroid variant of Ehlers-Danlos syndrome, progeria, familial tumoral calcinosis, Schneckenbecken dysplasia, Walker-Warburg syndrome, spondylocostal dysostosis type 3, and Peter's plus syndrome. Causes of these diseases include gene mutations and deficiency of proteins (enzymes). Their diagnosis includes syndromic presentation, organ-specific expression and laboratory findings.
Effect of stimulation of trigeminal ganglion on regional cerebral blood flow in cats (1987)

Regional cerebral blood flow was studied in the cat, with and without trigeminal ganglion stimulation, by the intravenous injection of the tracer [14C]iodoantipyrine and subsequent regional brain dissection. Electrical activation of the trigeminal ganglion led to a selective increase in regional blood flow in the frontal and parietal cortex that was bilateral without change in the posterior cortex, deep cerebral nuclei, white matter, or brain stem. Unilateral intracranial section of the facial nerve blocked the response in the ipsilateral frontal and parietal cortex, whereas bilateral facial nerve section blocked the contralateral frontal cortical response. The contralateral parietal cortical increase in blood flow was not affected by facial nerve section and may thus represent the result of metabolic activation of sensory cortex.
Asterric acid, a new endothelin binding inhibitor. (1992)

Endothelin (ET) is a potent vasoconstrictor/ pressor peptide consisting of 21 amino acid residues isolated from the cultures of porcine aortic endothelial cells1}. Analysis of a human genomic
Isogenic Pairs of Wild Type and Mutant Induced Pluripotent Stem Cell (iPSC) Lines from Rett Syndrome Patients as In Vitro Disease Model (2011)

Rett syndrome (RTT) is an autism spectrum developmental disorder caused by mutations in the X-linked methyl-CpG binding protein 2 (MECP2) gene. Excellent RTT mouse models have been created to study the disease mechanisms, leading to many important findings with potential therapeutic implications. These include the identification of many MeCP2 target genes, better understanding of the neurobiological consequences of the loss- or mis-function of MeCP2, and drug testing in RTT mice and clinical trials in human RTT patients. However, because of potential differences in the underlying biology between humans and common research animals, there is a need to establish cell culture-based human models for studying disease mechanisms to validate and expand the knowledge acquired in animal models. Taking advantage of the nonrandom pattern of X chromosome inactivation in female induced pluripotent stem cells (iPSC), we have generated isogenic pairs of wild type and mutant iPSC lines from several female RTT patients with common and rare RTT mutations. R294X (arginine 294 to stop codon) is a common mutation carried by 5-6% of RTT patients. iPSCs carrying the R294X mutation has not been studied. We differentiated three R294X iPSC lines and their isogenic wild type control iPSC into neurons with high efficiency and consistency, and observed characteristic RTT pathology in R294X neurons. These isogenic iPSC lines provide unique resources to the RTT research community for studying disease pathology, screening for novel drugs, and testing toxicology.
SHORTER NOTICES (1993)

SHORTER NOTICES Get access RUTH HARRIS RUTH HARRIS New CollegeOxford Search for other works by this author on: Oxford Academic Google Scholar The English Historical Review, Volume CVIII, Issue 428, July 1993, Pages 744-b–745, https://doi.org/10.1093/ehr/CVIII.428.744-b Published: 01 July 1993
New Gaussian-based approximation for modelling non-linear effects in optical fibers (1994)

The use of Gaussian field approximations enables simple analytical models to be derived for non-linear fibers and doped-fiber lasers and amplifiers. A new Gaussian-based method is described that is as easy to use as the standard Gaussian approximations, yet provides an rms error in the confinement factor of only 1.1% for a wide range of matched-cladding fibers. Similar accuracy is obtained for depressed-cladding fibers. The new approximation is used to predict Raman gain in a typical experimental MCVD fiber and excellent agreement is obtained between theory and experimental measurements.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>
Joint Antenna Selection and Beamforming Algorithms for Physical Layer Multicasting with Massive Antennas (2016)

We investigate the problem of minimizing the total power consumption under the constraint of the signal-to-noise ratio (SNR) requirement for the physical layer multicasting system with large-scale antenna arrays. In contrast with existing work, we explicitly consider both the transmit power and the circuit power scaling with the number of antennas. The joint antenna selection and beamforming technique is proposed to minimize the total power consumption. The problem is a challenging one, which aims to minimize the linear combination of ℓ 0 -norm and ℓ 2 -norm. To our best knowledge, this minimization problem has not yet been well solved. A random decremental antenna selection algorithm is designed, which is further modified by an approximation of the minimal transmit power based on the asymptotic orthogonality of the channels. Then, a more efficient decremental antenna selection algorithm is proposed based on minimizing the ℓ 0 norm. Performance results show that the ℓ 0 norm minimization algorithm greatly outperforms the random selection algorithm in terms of the total power consumption and the average run time.
Postfabrication resonance peak positioning of long-period cladding-mode-coupled gratings (1996)

A simple, flexible method of postfabrication positioning of resonance wavelengths of long-period cladding-modecoupled gratings is proposed. This method is based on changing the outer fiber diameter. Reducing the diameter by etching the fiber in HF acid shifts the loss peaks to higher wavelengths. A shift as large as 130 nm after 5 min of HF etching was observed for our strongest grating peak, corresponding to the cladding mode HE19. The experimental results are in excellent agreement with model calculations.
Akt-Mediated Survival of Oligodendrocytes Induced by Neuregulins (2000)

Neuregulins have been implicated in a number of events in cells in the oligodendrocyte lineage, including enhanced survival, mitosis, migration, and differentiation. At least two signaling pathways have been shown to be involved in neuregulin signaling: the phosphatidylinositol (PI)-3 kinase and the mitogen-activated protein kinase pathways. In the present studies, we examined the signaling pathway involved in the survival function of heregulin, focusing on heregulin-induced changes in Akt activity in cultured glial cells, and the consequences of Akt activation in cells in the oligodendrocyte lineage. Heregulin binds erbB receptors, and in our studies, primary cultures of both oligodendrocyte progenitor cells and differentiating oligodendrocytes expressed erbB2, erbB3, and erbB4 receptors. In C6 glioma cells and primary cultures of oligodendrocytes, heregulin induced time- and dose-dependent Akt phosphorylation at Ser(473) in a wortmannin-sensitive manner. To investigate further the signaling pathway for heregulin in glial cells, BAD was overexpressed in C6 glioma cells. In these cells, heregulin induced phosphorylation of BAD at Ser(136). Apoptosis of oligodendrocyte progenitor cells induced by growth factor deprivation was effectively blocked by heregulin in a wortmannin-sensitive manner. Overexpression of dominant negative Akt but not of wild-type Akt by adenoviral gene transfer in primary cultures of both oligodendrocytes and their progenitors induced significant apoptosis through activation of the caspase cascade. The present data suggest that the survival function of heregulin is mediated through the PI-3 kinase/Akt pathway in cells in the oligodendrocyte lineage and that the Akt pathway may be quite important for survival of cells in this lineage.
Proteasome inhibition by the natural products epoxomicin and dihydroeponemycin: Insights into specificity and potency (1999)

While two structurally related epoxyketone-containing antitumor natural products, epoxomicin and eponemycin, share the proteasome as a common intracellular target, they differ in their antiproliferative activity, proteasome subunit binding specificity, and rates of proteasome inhibition. As a first step towards understanding such differences and developing novel proteasome subunit-specific inhibitors, we report here the synthesis and characterization of epoxomicin/dihydroeponemycin chimerae.
Pineal Melatonin: Cell Biology of Its Synthesis and of Its Physiological Interactions* (1991)

UNTIL 35 yr ago, most scientists did not take research on the pineal gland seriously. The decade beginning in 1956, however, provided several discoveries that laid the foundation for what has become a very active area of investigation. These important early observations included the findings that, 1), the physiological activity of the pineal is influenced by the photoperiodic environment (1–5); 2), the gland contains a substance, N-acetyl-5-methoxytryptamine or melatonin, which has obvious endocrine capabilities (6, 7); 3), the function of the reproductive system in photoperiodically dependent rodents is inextricably linked to the physiology of the pineal gland (5, 8, 9); 4), the sympathetic innervation to the pineal is required for the gland to maintain its biosynthetic and endocrine activities (10, 11); and 5), the pineal gland can be rapidly removed from rodents with minimal damage to adjacent neural structures using a specially designed trephine (12). Since the mid 1960s, research on the pineal gland has increased exponentially, and its association with a wide variety of physiological systems has been documented (13–18). Proof that melatonin is the hormone of pineal origin that accounts for many of the endocrine manifestations of the gland, however, came somewhat later. Thus, whereas some early studies certainly suggested that melatonin had modulatory effects on the neuroendocrine- reproductive axis (19, 20), these actions were questioned when it was observed that in hamsters bearing sc placed melatonin pellets, which release the indole continuously, the ability of short day exposure and the pineal to suppress reproductive physiology was unexpectedly negated (21–23). These observations were, however, followed closely by studies showing that melatonin, administered as a single daily injection at a precise time with regard to the light-dark cycle, induced quiescence of the neuroendocrine-reproductive axis just as did short day exposure (24, 25).
Biopharmaceutical studies of 3-substituted isatin derivatives. (1990)

The metabolic fate of isatin hydrazone (Ia), isatin-3-thiosemicarbazone (Ib), isatin-3-semicarbazone (Ic), isatin-3-phenylhydrazone (Id), isatin oxime (Ie) and 3-hydroxy-3-acetonyl oxindole (II) was studied in rabbits. The compounds were administered orally in the dose of 300 mg/kg body wt. Isatin anthranilic acid, tryptophan and nicotinic acid were identified as the major metabolites excreted in urine. The 3-hydroxy-3-acetonyl oxindole (II) gave on additional metabolite, oxindole. The major metabolites were separated and identified unambiguously on thin layer silica gel plate. Metabolic pathways have been proposed to explain the biotransformation of the compounds investigated.
The actin gene promoter-driven bar as a dominant selectable marker for nuclear transformation of Dunaliella salina. (2005)

A 5'-flanking region of an actin gene from the green unicellular alga Dunaliella salina (D. salina) was cloned using a genome-walking method by PCR and its structural features were characterized. Two repetitive sequences found, over 75 bp in length each, were located at position -573 and -424 bp,respectively, relative to the AUG codon. The actin gene promoter region of D. salina displayed a consensus sequence of GCTC (G/C) AAGGC, a CCAAT motif and two TATA-like motifs that did not have a canonical sequence of a TATA box. The 5' flanking region of the actin gene was exploited to direct expression of the bialaphos resistance gene (bar) from Streptomyces hygroscopicus as a dominant marker in the nuclear transformation of D. salina. Direct selection of bar resistant transformants was achieved by allowing a 24 h period of recovery of cells transformed by biolistic procedure, followed by growth of the cells for one week under standard condition prior to harvesting and plating on the solid medium containing 0.5 microg/mL of phosphinothricin (PPT). Five colonies picked from the plate were analyzed, of which the integration of the bar gene was demonstrated in the nuclear genome. Southern blotting revealed that only one of five transformants contained a single copy of the bar gene whereas others contained multiple copies,suggesting that nuclear transformation of D. salina mainly occurred through illegitimate recombination events,resulting in ectopic integration of the introduced DNA. The integration patterns of the foreign DNA in this experiment appeared not to influence the bar gene expression in the transformants containing single or multiple inserts. The bar gene expression in the five transformants was verified by RT-PCR, confirming transcription of the chimeric DNA. These transformants were maintained on agar plates in the absence of PPT for more than seven months and retained resistance to the herbicide at 1 microg/mL. This work demonstrates that the actin gene promoter-driven expression of the bar gene may be used as a dominant selectable marker for nuclear transformation of D. salina.
3D database searching in drug design (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT3D database searching in drug designYvonne C. MartinCite this: J. Med. Chem. 1992, 35, 12, 2145–2154Publication Date (Print):June 1, 1992Publication History Published online1 May 2002Published inissue 1 June 1992https://pubs.acs.org/doi/10.1021/jm00090a001https://doi.org/10.1021/jm00090a001research-articleACS PublicationsRequest reuse permissionsArticle Views804Altmetric-Citations192LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Hybrid Segmentation Framework for Computer-Assisted Dental Procedures (2009)

Teeth segmentation in computed tomography (CT) images is a major and challenging task for various computer assisted procedures. In this paper, we introduced a hybrid method for quantification of teeth in CT volumetric dataset inspired by our previous experiences and anatomical knowledge of teeth and jaws. In this regard, we propose a novel segmentation technique using an adaptive thresholding, morphological operations, panoramic re-sampling and variational level set algorithm. The proposed method consists of several steps as follows: first, we determine the operation region in CT slices. Second, the bony tissues are separated from other tissues by utilizing an adaptive thresholding technique based on the 3D pulses coupled neural networks (PCNN). Third, teeth tissue is classified from other bony tissues by employing panorex lines and anatomical knowledge of teeth in the jaws. In this case, the panorex lines are estimated using Otsu thresholding and mathematical morphology operators. Then, the proposed method is followed by calculating the orthogonal lines corresponding to panorex lines and panoramic re-sampling of the dataset. Separation of upper and lower jaws and initial segmentation of teeth are performed by employing the integral projections of the panoramic dataset. Based the above mentioned procedures an initial mask for each tooth is obtained. Finally, we utilize the initial mask of teeth and apply a variational level set to refine initial teeth boundaries to final contour. In the last step a surface rendering algorithm known as marching cubes (MC) is applied to volumetric visualization. The proposed algorithm was evaluated in the presence of 30 cases. Segmented images were compared with manually outlined contours. We compared the performance of segmentation method using ROC analysis of the thresholding, watershed and our previous works. The proposed method performed best. Also, our algorithm has the advantage of high speed compared to our previous works.