gebhart/patrepeval huggingface dataset:
Acoustic wave viscosity sensor (1987)

An acoustic wave device utilizing plate modes having components of displacement parallel to a crystal surface has been demonstrated to be an effective sensor of liquid shear viscosity (η) over a wide viscosity range. When a liquid is present on the sensor surface, the propagation loss of the acoustic wave depends upon η in a calculable fashion. Because the device functions at 159 MHz, liquid relaxation effects occur with high viscosity liquids, causing the propagation loss to saturate. Viscosity is sampled in the 50-nm-thick liquid layer which couples to the acoustic wave, so that only a single drop of liquid is required for measurement.
Remote Query Resonant-Circuit Sensors for Monitoring of Bacteria Growth: Application to Food Quality Control (2002)

This paper presents a technique for in-situ remote query monitoring of bacteria growth utilizing a printed thin or thick-film sensor comprised of an inductor-capacitor (LC) resonant circuit. The sensor, which is placed within the biological medium of interest and remotely detected using a loop antenna, measures the complex permittivity of the medium. Since bacteria growth increases the complex permittivity of a biological medium the LC sensor can be used to determine bacteria concentration. This paper presents results on monitoring of three different bacteria strains, Bacillus subtilis, Escherichia coli JM109, and Pseudomonas putida, demonstrating application of the sensor for monitoring bacteria growth in milk, meat, and beer. Due to its low unit cost and remote query detection, the sensor is potentially useful for commercial scale monitoring of food quality.
Pyoverdin is essential for virulence of Pseudomonas aeruginosa (1996)

The role of pyoverdin, the main siderophore in iron-gathering capacity produced by Pseudomonas aeruginosa, in bacterial growth in vivo is controversial, although iron is important for virulence. To determine the ability of pyoverdin to compete for iron with the human iron-binding protein transferrin, wild-type P. aeruginosa ATCC 15692 (PAO1 strain) and PAO pyoverdin-deficient mutants were grown at 37 degrees C in bicarbonate-containing succinate medium to which apotransferrin had been added. Growth of the pyoverdin-deficient mutants was fully inhibited compared with that of the wild type but was restored when pyoverdin was added to the medium. Moreover, when growth took place at a temperature at which no pyoverdin production occurred (43 degrees C), the wild-type PAO1 strain behaved the same as the pyoverdin-deficient mutants, with growth inhibited by apotransferrin in the presence of bicarbonate and restored by pyoverdin supplementation. Growth inhibition was never observed in bicarbonate-free succinate medium, whatever the strain and the temperature for growth. In vivo, in contrast to results obtained with the wild-type strain, pyoverdin-deficient mutants demonstrated no virulence when injected at 10(2) CFU into burned mice. However, virulence was restored when purified pyoverdin originating from the wild-type strain was supplemented during the infection. These results strongly suggest that pyoverdin competes directly with transferrin for iron and that it is an essential element for in vivo iron gathering and virulence expression in P. aeruginosa. Rapid removal of iron from [59Fe]ferritransferrin by pyoverdin in vitro supports this view.
RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA. (1983)

The RNA homologous to the yeast transposable element Ty1 is one of the more abundant poly(A)+ RNAs in many strains of the yeast Saccharomyces cerevisiae. The 5' and 3' ends of Ty1 RNA have been determined from analysis of cDNA. The 5' end is 245 bases into the left delta sequence measured from the left side of the Ty1 element. The delta sequence is a direct repeat of about 340 base pairs present at each end of the Ty1 element. The Ty1 transcription includes 93-97 bases of the left delta sequence and continues through the entire internal portion of the element and through about 295 bases of the right delta sequence before reaching the 3' end located 38-46 bases from the right side of the right delta sequence. Because the delta sequences present at each end of a single Ty1 element have identical or very similar DNA sequences, these end points for Ty1 RNA raise several questions about the expression of Ty1 elements. First, what are the initiation and termination signals, because the Ty1 transcript must read through a DNA sequence that is identical to the 3' end at about 50 bases from the 5' end? Second, why is the direction of transcription of the Ty1 element opposite to that of genes that are overexpressed after the insertion of a Ty1 element? Third, because the Ty1 RNA itself has direct repeats of about 45 bases, a structure analogous to retrovirus RNAs, is the Ty1 RNA an intermediate in the transposition of Ty1?
Potent Inhibitors of the Hedgehog Signaling Pathway (2008)

A small family of phenyl quinazolinone ureas is reported as potent modulators of Hedgehog protein function. Preliminary SAR studies of the urea substituent led to a nanomolar Hedgehog antagonist.
Plasma amino acids in a patient with Wilson's disease presenting with an acute haemolytic anaemia (1980)

Abstract A 10‐year‐old girl with Wilson's disease presented with an acute haemolytic anaemia. Plasma amino acids, studied during two haemolytic episodes, were abnormal, especially cystine, which was increased approximately tenfold.
Mesoporous ferrihydrite with incorporated manganese for rapid removal of organic contaminants in air (2012)

Mesoporous ferrihydrite nanoparticles with incorporated Mn (MMnFh) were prepared for the first time, and MMnFh exhibits excellent performance in removing organic contaminants (CH(3)CHO and toluene) in air. The interesting physico-chemical properties and remarkable activity of MMnFh give great commercial potential in air purification application in diverse sectors.
An Analytical Algorithm for Placement of Arbitrarily Sized Rectangular Blocks (1985)

This paper presents a novel mathematical formulation to the placement of arbitrarily sized rectangles that simultaneously account for the topological and geometrical characteristics of the placement problem. Special attention is paid to maintain the continuity of the formulation. Two examples are given to demonstrate the performance of the algorithm. Identical placement is obtained from two different initial placements of an example which has 8 movable components and 29 signal nets. For a practical example with 15 movable blocks and 142 signal nets, a final placement which is manual solution comparable is obtained using random initial positions.
The secretory core protein of human hepatitis B virus is expressed on the cell surface (1989)

Human hepatitis B virus (HBV) causes acute and chronic liver disease, which can result in tumor formation. An as yet unexplained phenomenon is that virus elimination usually correlates with the development of antibodies directed against the HBeAg, a secretory HBV core gene product which can be detected in the serum of infected patients. Expression of HBeAg in a human hepatoma cell line by using recombinant vaccinia viruses revealed that the HBeAg is not only secreted from HBeAg-producing cells but also incorporated into the outer cell membrane. No membrane-expressed core gene product could be detected when the cytoplasmic core protein (HBcAg) was expressed. Immune sera from patients who developed anti-HBe antibodies efficiently recognized the membrane-bound HBeAg, suggesting that surface-expressed HBeAg can serve as a target for an antibody-mediated elimination of HBV-infected cells.
Cutting Edge: TCR Revision Occurs in Germinal Centers (2004)

Mouse CD4(+)Vbeta5(+) T cells recognize a peripherally expressed superantigen encoded by an endogenous retrovirus. Ag encounter tolerizes the mature CD4 T cell compartment, either by deletion of autoreactive cells or by TCR revision. This latter process is driven by TCRbeta rearrangement through RAG activity and results in the rescue of cells expressing novel TCRs that no longer recognize the tolerogen. Consistent with the notion that revising T cells represent a distinct peripheral T cell population, we now show that these lymphocyte blasts express a hybrid effector/memory phenotype and are not undergoing cell division. A population of revising T cells is CD40(+), expresses the germinal center (GC) marker CXCR5, and is Vbeta5(low)Thy-1(low). Histology reveals that, consistent with their surface Ag phenotype, T cells undergoing TCR revision are enriched in splenic GCs. These data demonstrate that TCR revision is a multistep tolerance pathway supported by the unique microenvironment provided by GCs.
Isolation of Insulin-Like Growth Factors I and II from Human Plasma (1984)

Insulin-like growth factors I and II have been isolated from Cohn fraction IV-1 of human plasma using gel permeation chromatography, ion exchange chromatography, reversed phase chromatography, isoelectric focusing (IEF) and high performance liquid chromatography(HPLC). IGF I of specific activity 89 U/g, as measured by the isolated rat adipocyte assay, and IGF II, of specific activity 78 U/g, were obtained in yields of 16 micrograms and 34 micrograms respectively per 100g of Cohn fraction. Although this process yields IGF I which is contaminated with IGF II (due to the relatively large amount of the latter present in the original plasma), the IGF II preparations appear to be relatively free from IGF I. This separation was mainly achieved with IEF since the two factors elute close together on HPLC. Nevertheless, HPLC is important for their subsequent purification. The process is thus especially suitable for the preparation of IGF II and appears to give better yields than those obtained by earlier methods which used acid-ethanol extraction, gel permeation chromatography and polyacrylamide gel electrophoresis.
A biomimetic strategy in the synthesis and fragmentation of cyclic protein (1998)

This paper describes a simple biomimetic strategy to prepare small cyclic proteins containing multiple disulfide bonds. Our strategy involves intramolecular acyl transfer reactions to assist both the synthesis and fragmentation of these highly constrained cyclic structures in aqueous solution. To illustrate our strategy, we synthesized the naturally occurring circulin B and cyclopsychotride (CPT), both consisting of 31 amino acid residues tightly packed in a cystine-knot motif with three disulfide bonds and an end-to-end cyclic form. The synthesis of these small cyclic proteins can be achieved by orthogonal ligation of free peptide thioester via the thia zip reaction, which involves a series of reversible thiol-thiolactone exchanges to arrive at an alpha-amino thiolactone, which then undergoes an irreversible, spontaneous ring contraction through an S,N-acyl migration to form the cyclic protein. A two-step disulfide formation strategy is employed for obtaining the desired disulfide-paired products. Partial acid hydrolysis through intramolecular acyl transfer of X-Ser, X-Thr, Asp-X, and Glu-X sequences is used to obtain the assignment of the circulins disulfide bond connectives. Both synthetic circulin B and CPT are identical to the natural products and, thus, the total synthesis confirms the disulfide connectivity of circulin B and CPT contain a cystine-knot motif of 1-4, 2-5, and 3-6. In general, our strategy, based on the convergence of chemical proteolysis and aminolysis of peptide bonds through acyl transfer, is biomimetic and provides a useful approach for the synthesis and characterization of large end-to-end cyclic peptides and small proteins.
Iodide Uptake and Experimental 131I Therapy in Transplanted Undifferentiated Thyroid Cancer Cells Expressing the Na+/I− Symporter Gene (1997)

131I therapy is a widely accepted treatment for differentiated thyroid cancers which can accumulate iodide. We evaluated the efficiency of 131I therapy against tumors which are transfected with the Na+/I- symporter (NIS) gene. We transfected the rat NIS cDNA expression vector into malignantly transformed rat thyroid cells (FRTL-Tc) which do not concentrate iodide. The resultant cell line (Tc-rNIS) accumulated 125I 60-fold in vitro. The FRTL-Tc cells formed solid tumors after injection of cells into subcutaneous tissues of Fischer 344 rats. Tumors formed with Tc-rNIS cells accumulated up to 27.3% of total 125I administered, and concentrated 125I 11 to 27-fold in the tumors. Extracorporeal measurement of radioactivity in the tumors revealed that 125I accumulation peaked at 90 min, and decreased to half levels 6 h after the injections. To investigate the effect of 131I administration on the tumor growth, we injected Na131I 2 and 3 weeks after the transplantation of the cells. The Na131I did not change the tumor volume significantly in either the FRTL-Tc or the Tc-rNIS-induced tumors. The short (6 h) effective half life of 131I in the tumors diminished the radiation dose to the tumor cells. However, this approach may prove beneficial in the treatment of radiosensitive cancers, and could be employed diagnostically.
Leukocyte composition of human breast cancer (2011)

Retrospective clinical studies have used immune-based biomarkers, alone or in combination, to predict survival outcomes for women with breast cancer (BC); however, the limitations inherent to immunohistochemical analyses prevent comprehensive descriptions of leukocytic infiltrates, as well as evaluation of the functional state of leukocytes in BC stroma. To more fully evaluate this complexity, and to gain insight into immune responses after chemotherapy (CTX), we prospectively evaluated tumor and nonadjacent normal breast tissue from women with BC, who either had or had not received neoadjuvant CTX before surgery. Tissues were evaluated by polychromatic flow cytometry in combination with confocal immunofluorescence and immunohistochemical analysis of tissue sections. These studies revealed that activated T lymphocytes predominate in tumor tissue, whereas myeloid lineage cells are more prominant in "normal" breast tissue. Notably, residual tumors from an unselected group of BC patients treated with neoadjuvant CTX contained increased percentages of infiltrating myeloid cells, accompanied by an increased CD8/CD4 T-cell ratio and higher numbers of granzyme B-expressing cells, compared with tumors removed from patients treated primarily by surgery alone. These data provide an initial evaluation of differences in the immune microenvironment of BC compared with nonadjacent normal tissue and reveal the degree to which CTX may alter the complexity and presence of selective subsets of immune cells in tumors previously treated in the neoadjuvant setting.
Molecular Cloning of a Non-inactivating Proton-gated Na+ Channel Specific for Sensory Neurons (1997)

We have cloned and expressed a novel proton-gated Na+ channel subunit that is specific for sensory neurons. In COS cells, it forms a Na+ channel that responds to a drop of the extracellular pH with both a rapidly inactivating and a sustained Na+ current. This biphasic kinetic closely resembles that of the H+-gated current described in sensory neurons of dorsal root ganglia (1). Both the abundance of this novel H+-gated Na+ channel subunit in sensory neurons and the kinetics of the channel suggest that it is part of the channel complex responsible for the sustained H+-activated cation current in sensory neurons that is thought to be important for the prolonged perception of pain that accompanies tissue acidosis (1, 2).
Fabrication, structure, and properties of chitin whisker‐reinforced alginate nanocomposite fibers (2008)

Abstract Calcium alginate yarn (30 fibers) and calcium alginate nanocomposite yarn (30 fibers) containing 0.05–2.00% w/w chitin whiskers were both prepared by wet spinning process. The whiskers were prepared by acid hydrolysis of chitin from shrimp shells. The average length and width of the whiskers were 343 and 46 nm, with the aspect ratio being ∼ 7.5. Incorporation of a low amount of the whiskers in the nanocomposite fibers improved both the mechanical and the thermal properties of the fibers significantly, possibly a result of the specific interactions, i.e., hydrogen bonding and electrostatic interactions, between the alginate molecules and the homogeneously dispersed chitin whiskers. Biodegradation of the calcium alginate fibers and the nanocomposite fibers was tested in Tris‐HCl buffer solution and the same buffer solution that contained lysozyme. The addition of the chitin whiskers in the nanocomposites fibers accelerated the biodegradation process of the fibers in the presence of lysozyme, whereas the presence of Ca 2+ ions in the Tris‐HCl buffer solution helped to improve the tenacity of both the alginate and the nanocomposite fibers. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Non-invasive Studies on Age Related Parameters Using a Blood Volume Pulse Sensor (2008)

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Efficient Conversion of Carboxylic Acids intoN-Acylbenzotriazoles (2003)

An improved one-pot procedure for the preparation of N-acylbenzotriazoles involves mild reaction conditions and allows the preparation of several derivatives not accessible by the previously reported methods.
Copolymers of modified starches with polyacrylonitrile (1969)

Abstract A study was made of the ceric ammonium nitrate‐initiated graft polymerization of acrylonitrile (AN) onto a number of modified starches that had been reduced in molecular weight by either acid, hypochlorite, or enzyme treatment. With highly soluble starches, much of the starting material was recovered as ungrafted carbohydrate, and the reaction product was largely dimethylformamide‐soluble polymer with a high polyacrylonitrile (PAN) content. The molecular weight of grafted PAN was lower when the modified starches existed as granules in water dispersion; however, heating (60°C) an aqueous slurry of an acid‐modified corn starch (with intact granules) before the reaction had relatively little effect on the composition of the copolymer. Decreasing the concentrations in water of modified starch and AN resulted in more frequent and lower molecular weight grafts of PAN. Aqueous methanol as a reaction medium for an acid‐modified starch with intact granules led to more frequent grafting of lower molecular weight PAN than when water alone was used. The number of grafted chains, however, was fewer than found with unmodified wheat starch under comparable conditions. A modified starch with the granule structure completely broken down gave no detectable reaction in aqueous methanol.
AGENT'S REPUTATION, ACCOUNTING AND COSTING IN ORGANISATIONAL CONTROL STRUCTURES (1997)

This paper examines the roles of accounting and costing in the management of coal mining during the Industrial Revolution in Britain, and considers the impact of the agent's reputation in the development and use of these systems.
Multi-layer interaction for digital tables (2006)

Interaction on digital tables has been restricted to a single layer on the table's active work-surface. We extend the design space of digital tables to include multiple layers of interaction. We leverage 3D position information of a pointing device to support interaction in the space above the active work-surface by creating multiple layers with drift-correction in which the user can interact with an application. We also illustrate through a point-design that designers can use multiple-layers to create a rich and clutter free application. A subjective evaluation showed that users liked the interaction techniques and found that, because of the drift correction we use, they could control the pointer when working in any layer.
Cyberwalk: a web-based distributed virtual walkthrough environment (2003)

A distributed virtual walkthrough environment allows users connected to the geometry server to walk through a specific place of interest, without having to travel physically. This place of interest may be a virtual museum, virtual library or virtual university. There are two basic approaches to distribute the virtual environment from the geometry server to the clients, complete replication and on-demand transmission. Although the on-demand transmission approach saves waiting time and optimizes network usage, many technical issues need to be addressed in order for the system to be interactive. CyberWalk is a web-based distributed virtual walkthrough system developed based on the on-demand transmission approach. It achieves the necessary performance with a multiresolution caching mechanism. First, it reduces the model transmission and rendering times by employing a progressive multiresolution modeling technique. Second, it reduces the Internet response time by providing a caching and prefetching mechanism. Third, it allows a client to continue to operate, at least partially, when the Internet is disconnected. The caching mechanism of CyberWalk tries to maintain at least a minimum resolution of the object models in order to provide at least a coarse view of the objects to the viewer. All these features allow CyberWalk to provide sufficient interactivity to the user for virtual walkthrough over the Internet environment. In this paper, we demonstrate the design and implementation of CyberWalk. We investigate the effectiveness of the multiresolution caching mechanism of CyberWalk in supporting virtual walkthrough applications in the Internet environment through numerous experiments, both on the simulation system and on the prototype system.
Synthesis of Ruthenium Hydride Complexes Containing beta‐Aminophosphine Ligands Derived from Amino Acids and their use in the H2‐Hydrogenation of Ketones and Imines (2005)

Abstract The new complexes RuHCl(PPh 2 CH 2 CHRNH 2 ) 2 and RuHCl(PPh 2 CH 2 CHRNH 2 )(R‐ binap), R=H (Pgly), R=Me [( R )‐Pala] were prepared by the substitution of the PPh 3 ligands in RuHCl(PPh 3 ) 3 or RuHCl(PPh 3 )[( R )‐binap] with beta‐aminophosphines derived from amino acids. The complex trans ‐RuHCl(Pgly)[( R )‐binap] has been characterized by X‐ray crystallography. The complex trans ‐RuHCl[( S )‐Ppro] 2 where ( S )‐Ppro is derived from proline was also prepared and characterized by X‐ray crystallography. These were used as catalyst precursors in the presence of a base (KOPr‐ i or KOBu‐ t ) for the hydrogenation of various ketones and imines to the respective alcohols and amines with H 2 gas (1–11 atm) at room temperature. Acetophenone was hydrogenated to ( S )‐1‐phenylethanol in low ee (up to 40%) when catalyzed by the enantiomerically pure complexes. These complexes are especially active in the hydrogenation of sterically congested and electronically deactivated ketones and imines and are selective for the hydrogenation of CO bonds over CC bonds.