gebhart/patrepeval huggingface dataset:
The sequence of the zein regulatory geneopaque-2 (O2) ofZea Mays (1989)

Journal Article The sequence of the zein regulatory gene opaque -2 ( O2 ) of Zea Mays Get access M. Maddaloni, M. Maddaloni istituto Sperimentale per la CerealicolturaVia Stezzano 24 24100 Bergamo, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar N. Di Fonzo, N. Di Fonzo istituto Sperimentale per la CerealicolturaVia Stezzano 24 24100 Bergamo, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Hartings, H. Hartings istituto Sperimentale per la CerealicolturaVia Stezzano 24 24100 Bergamo, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar N. Lazzaroni, N. Lazzaroni istituto Sperimentale per la CerealicolturaVia Stezzano 24 24100 Bergamo, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar F. Salamini, F. Salamini 1Max-Planck Institut fü;r ZüchtungsforschungD-5000 Köln, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar R. Thompson, R. Thompson istituto Sperimentale per la CerealicolturaVia Stezzano 24 24100 Bergamo, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar M. Motto M. Motto istituto Sperimentale per la CerealicolturaVia Stezzano 24 24100 Bergamo, Italy Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 17, Issue 18, 25 September 1989, Pages 7532–7533, https://doi.org/10.1093/nar/17.18.7532 Published: 25 September 1989 Article history Received: 17 July 1989 Published: 25 September 1989
Phase-shifting point diffraction interferometer (1996)

We describe a novel interferometer design suitable for highly accurate measurement of wave-front aberrations over a wide range of wavelengths, from visible to x ray. The new design, based on the point diffraction interferometer, preserves the advantages of the conventional point diffraction interferometer but offers higher efficiency and improved accuracy through phase shifting. These qualities make it applicable to at-wavelength testing of many optical systems, including short-wavelength projection lithography optics. A visible-light prototype was built and operated.
Evidence for T Cell-dependent Immunity to Bacteroides fragilis in an Intraabdominal Abscess Model (1982)

It has been shown that active immunization of rats with the capsular polysaccharide of Bacteroides fragilis protects these animals against abscess development following intraperitoneal challenge with this species. Passive transfer of hyperimmune globulin from immunized animals to nonimmune recipients provided protection against B. fragilis bacteremia in challenged animals, but did not confer protection against abscess development. On the other hand, adoptive transfer of spleen cells from immunized animals to nonimmunized recipients resulted in protection against abscesses following challenge with B. fragilis. These data suggested that a T cell-dependent immune response was involved in protection against abscess development after immunization with B. fragilis capsular antigen. To determine the possible role of cell-mediated immunity prompted by the capsular antigen, inbred congenitally athymic OLA/Rnu rats and their phenotypically normal littermates were actively immunized. Despite the development of high titers of anti-B. fragilis capsular antibody, 100% of actively immunized athymic rats developed abscesses, as did 100% of unimmunized athymic control rats. However, no phenotypically normal littermate control rats that were actively immunized developed abscesses, while 100% of phenotypically normal unimmunized rats developed abscesses. Additional studies showed that adoptive transfer of T cell-enriched spleen cell preparations from Wistar/Lewis rats immunized with the capsular polysaccharide to nonimmune recipients also resulted in protection against B. fragilis-induced abscesses. We conclude that the protection afforded by immunization with B. fragilis capsule against intraabdominal abscesses caused by that organism is T cell-mediated and does not require the presence of serum antibody.
Tissue engineering and the road to whole organs (2012)

A glimpse into the future of surgery
Derivation and characterization of cholesterol‐independent non‐GS NS0 cell lines for production of recombinant antibodies (2006)

Presented is an antibody production platform based on the fed-batch culture of recombinant NS0-derived cell lines. NS0 host cells, obtained from the European Collection of Cell Cultures (ECACC, Salisbury, UK, Part No. 85110503), were first adapted to grow in a protein-free, cholesterol-free medium. The resulting host cell line was designated NS0-PFCF (protein-free, cholesterol-free). The five production cell lines presented here were generated using a common protocol consisting of transfection by electroporation and subcloning. The NS0-PFCF host cell line was transfected using a single expression vector containing the Escherichia coli xanthine-guanine phosphoribosyl transferase gene (gpt), and the antibody heavy and light chain genes driven by the CMV promoter. The five cell lines were chosen after one to three rounds of iterative subcloning, which resulted in a 19-64% increase in antibody productivity when four mother-daughter cell pairs were cultured in a fed-batch bioreactor process. The production cell lines were genetically characterized to determine antibody gene integrity, nucleotide sequences, copy number, and the number of insertion sites in the NS0 cell genome. Genetic characterization data indicate that each of the five production cell lines has a single stably integrated copy of the antibody expression vector, and that the antibody genes are correctly expressed. Stability of antibody production was evaluated for three of the five cell lines by comparing the early stage seed bank with the Working Cell Bank (WCB). Antibody productivity was shown to be stable in two of three cell lines evaluated, while one of the cell lines exhibited a 20% drop in productivity after passaging for approximately 4 weeks. These five NS0-derived production cell lines were successfully cultured to produce antibodies with acceptable product quality attributes in a standardized fed-batch bioreactor process, consistently achieving an average specific productivity of 20-60 pg/cell-day, and a volumetric productivity exceeding 120 mg/L-day (Burky et al., 2006). In contrast to the commonly available NS0 host cell line, which requires serum and cholesterol for growth, and the commonly used expression vector system, which uses a proprietary glutamine synthetase selection marker (GS-NS0), these NS0 cells are cholesterol-independent, grow well in a protein-free medium, use a non-proprietary selection marker, and do not require gene amplification for productivity improvement. These characteristics are advantageous for use of this NS0 cell line platform for manufacturing therapeutic antibodies.
Peptide nucleic acid (PNA)/DNA hybrid duplexes: intercalation by an internally linked anthraquinone (1998)

Peptide nucleic acids (PNA) mimic DNA and RNA by forming complementary duplex structures following Watson-Crick base pairing. A set of reporter compounds that bind to DNA by intercalation are known, but these compounds do not intercalate in PNA/DNA hybrid duplexes. Analysis of the hybrid PNA duplexes requires development of reporter compounds that probe their chemical and physical properties. We prepared a series of anthraquinone (AQ) derivatives that are linked to internal positions of a PNA oligomer. These are the first non-nucleobase functional groups that have been incorporated into a PNA. The resulting PNA(AQ) conjugates form stable hybrids with complementary DNA oligomers. We find that when the AQ groups are covalently bound to PNA that they stabilize the hybrid duplex and are, at least partially, intercalated.
A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting (1996)

Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor alpha subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.
Immediate multi-threaded dynamic software updates using stack reconstruction (2009)

Software updates traditionally required applications to be stopped and restarted. Recent works proposed techniques and tools for runtime updates without the need for stop-ping and restarting applications. Such techniques rely on statically determining quiescent points where the global sys-tem state is consistent. However, quiescent points may be conservatively computed or may not occur frequently dur-ing the lifetime of an application resulting in an indefinite delay of runtime updates. Examples of applications with infrequent quiescent points include computationally-bound applications that spend most of their execution in loops, recursive applications, and multi-threaded applications. We introduce and implement a general approach that allows up-dates to be applied at almost any point during application execution. Unlike other existing approaches, our approach applies to multi-nested long-lived loops, recursive functions, multi-threaded applications, and applications actively re-ceiving data from a network socket. At the heart of our approach is a stack reconstruction technique that ensures that all functions on the call stack are updated at the same time, thereby obviating the need to wait for quiescent points to occur. Our updating system, UpStare, compiles appli-cations specifically with the capability to reconstruct their stack and does not depend on static source code type-safety analysis. Additionally it permits execution continuation to proceed from a drastically different algorithm while reusing existing program state.
Retinoic acid and interferon alpha act synergistically as antiangiogenic and antitumor agents against human head and neck squamous cell carcinoma. (1998)

Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy in which multiple independent lesions develop over time throughout the mucosa of the upper aerodigestive tract. Therefore, the comprehensive treatment of this neoplasm must include a chemopreventive arm to hold premalignant lesions in check, a role well-suited to antiangiogenic agents. Retinoic acid (RA) and interferon alpha (IFN-alpha), drugs with known biological activity against HNSCC when used individually, are also inhibitors of angiogenesis. Here we show that they are remarkably synergistic antiangiogenic agents able to inhibit both the growth and the neovascularization of HNSCC injected into the floor of the mouth of nude mice. The mechanism of action of these drugs as antiangiogenic agents was 2-fold. They decreased the angiogenic activity of the tumor cells, and they caused the endothelial cells to become refractory to inducers of angiogenesis. When tumor cells were treated in vitro with IFN-alpha A/D, there was a dramatic drop in their secretion of interleukin-8, the major angiogenic factor produced by these tumors. When combined with RA, which causes tumor cells to secrete an inhibitor of angiogenesis, there was a synergistic inhibition of both tumor cell growth and secreted angiogenic activity. The combination of RA and IFN-alpha also acted synergistically on endothelial cells by reducing their responsiveness to both interleukin-8 and tumor conditioned media. Doses of each drug could be reduced by two logs without loss of activity. When animals bearing human HNSCC tumor cells were treated systemically with a combination of RA and IFN-alpha A/D at doses that were ineffective when used alone, dramatic decreases in both tumor growth and tumor angiogenesis were seen. These data suggest that the use of antiangiogenic mixtures may be a particularly effective way to design future chemoprevention protocols against HNSCC.
Continuous free-flow electrophoresis separation of cytosolic proteins from the human colon carcinoma cell line LIM 1215: A non two-dimensional gel electrophoresis-based proteome analysis strategy (2001)

The conventional approach for analyzing the protein complement of a genome involves the combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometric based protein identification technologies. While 2-DE is a powerful separation technique, it is severely limited by the insolubility of certain classes of proteins (e.g. hydrophobic membrane proteins), as well as the amount of protein that can be processed. Here, we describe a simple procedure for resolving complex mixtures of proteins that involves a combination of free flow electrophoresis (FFE), a liquid-based isoelectric focussing (IEF) method, and sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Resolved proteins were identified by peptide fragment sequencing using capillary column reversed-phase high performance liquid chromatography (RP-HPLC)/mass spectrometry (MS). An initial demonstration of the method was performed using digitonin/ethylenediaminetetraacetic acid EDTA extracted cytosolic proteins from the human colon carcinoma cell line, LIM 1215. Cytosolic proteins were separated by liquid-based IEF (pH range 3–10) into 96 fractions, and each FFE fraction was further fractionated by SDS-PAGE. Selected protein bands were excised from the SDS-PAGE gel, digested in situ with trypsin, and subsequently identified by on-line RP-HPLC/electrospray-ionization ion trap MS. Our results indicate that FFE is: (i) an extremely powerful liquid-based IEF method for resolving proteins; (ii) not limited by the amount of sample that can be loaded onto the instrument; and (iii) capable of fractionating intact protein complexes (a potentially powerful tool for cell-mapping proteomics). An up-to-date list of cytosolic proteins from the human colorectal carcinoma cell line LIM 1215 can be found in the Joint Protein Structure Laboratory (JPSL) proteome database. This information will provide an invaluable resource for future proteomics-based biological studies of colon cancer. The JPSL proteome database can be accessed through the World Wide Web (WWW) network (http://www.ludwig.edu.au/jpsl/jpslhome.html).
Placenta Growth Factor Overexpression Inhibits Tumor Growth, Angiogenesis, and Metastasis by Depleting Vascular Endothelial Growth Factor Homodimers in Orthotopic Mouse Models (2006)

The role of placenta growth factor (PlGF) in pathologic angiogenesis is controversial. The effects of PlGF on growth, angiogenesis, and metastasis from orthotopic tumors are not known. To this end, we stably transfected three human cancer cell lines (A549 lung, HCT116 colon, and U87-MG glioblastoma) with human plgf-2 full-length cDNA. Overexpression of PlGF did not affect tumor cell proliferation or migration in vitro. The growth of PlGF-overexpressing tumors grown orthotopically or ectopically was impaired in all three tumor models. This decrease in tumor growth correlated with a decrease in tumor angiogenesis. The PlGF-overexpressing tumors had decreased vessel density and increased vessel diameter, but vessel permeability was not different from the parental tumors. Tumors overexpressing PlGF exhibited higher levels of PlGF homodimers and PlGF/vascular endothelial growth factor (VEGF) heterodimers but decreased levels of VEGF homodimers. Our study shows that PlGF overexpression decreases VEGF homodimer formation and inhibits tumor progression.
Myth and Reality (1992)

The chapter refutes the claims of both exclusive virtue and exclusive vice and points to certain common failings of humanity. It aims to demonstrate the myth and the truth behind each myth, such as the myth of Islamic racial innocence and the myth of total racial harmony in the Islamic world. These myths had a Western creator and they served a Western purpose. The author, in reviewing the evidence of prejudice and discrimination in the Middle Eastern part, discovers and tries to correct the false picture drawn by myth makers. There were obvious contrasts that led some European observers to defend Muslim slavery or to praise Muslim racial attitudes. One of these defenses was from Kremer. He abhorred slavery and described the abduction and transportation of African slaves in blood-chilling terms. However, he also took note that the slave was treated well once he reached his destination.
9,10-Dehydroanthracene. A derivative of 1,4-dehydrobenzene (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT9,10-Dehydroanthracene. A derivative of 1,4-dehydrobenzeneO. L. Chapman, C. C. Chang, and J. KolcCite this: J. Am. Chem. Soc. 1976, 98, 18, 5703–5705Publication Date (Print):September 1, 1976Publication History Published online1 May 2002Published inissue 1 September 1976https://pubs.acs.org/doi/10.1021/ja00434a054https://doi.org/10.1021/ja00434a054research-articleACS PublicationsRequest reuse permissionsArticle Views130Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
[Brown spider bite]. (1990)

The diagnosis of bite by the brown recluse spider, Loxosceles reclusus, is rarely based on absolute identification of the insect because the victims are usually bitten while sleeping or dressing. More often, the history, clinical findings and course of the bite lead to the diagnosis. For early confirmation up to 24 hours after the bite, the passive hemagglutination test can be used. For older lesions, the in-vitro lymphocyte transformation test is useful, but is available in only a few medical centers. Treatment of the bite of the brown recluse spider varies from conservative to more active approaches. Resting, local cooling, systemic antibiotics to prevent infection and mild anti-inflammatory drugs may be given. In the more active approach oral corticosteroids are added in the first 72 hours to the antibiotics, especially in massive bites with necrotic centers greater than 2 cm in diameter, or when there is systemic loxoscelism. Recently, good results have been reported with Avlosulfon (dapsone), which is claimed to cure necrotic cutaneous ulcerations, presumably by reducing the activity of polymorphonuclear leukocytes. Other treatments include specific antivenin, (of limited use because it must be administered shortly after the bite), and surgery to prevent spreading of the venom. We describe 3 cases of brown spider bite with typical clinical presentations in adults aged 20-40 years. 2 were treated with corticosteroids and antibiotics and 1 with Avlosulfon and prednisone, all within 72 hours of the bite. 2 recovered completely within a few days, but the third treated with prednisone and antibiotics, developed an ulcer which healed only after several months of treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
Molecular population genetic evidence of horizontal spread of two alleles of the pyrogenic exotoxin C gene (speC) among pathogenic clones of Streptococcus pyogenes (1992)

It has recently been demonstrated that the bacteriophage-borne gene (speC) encoding pyrogenic exotoxin C is harbored by phylogenetic lineages representing virtually the entire breadth of genomic differentiation present in the species Streptococcus pyogenes (J. M. Musser, A. R. Hauser, M. H. Kim, P. M. Schlievert, K. Nelson, and R. K. Selander, Proc. Natl. Acad. Sci. USA 88:2668-2672, 1991). To determine whether the speC genes occurring in association with divergent chromosomal genotypes (clones) are identical or represent a group of allelic variants, we sequenced speC from 23 S. pyogenes strains representing 15 clones identified by multilocus enzyme electrophoresis. Two alleles of speC are present in natural populations, and each allele occurs in clones that are well differentiated in overall chromosomal character; in one case, isolates of a single clone had different speC alleles. We interpret these patterns of toxin allele-clone distribution as evidence of occasional episodes of speC horizontal dissemination, presumably by bacteriophage-mediated gene transfer and recombination.
Epilog (1995)

In den zehn Kapiteln dieses Buches haben wir einige Aspekte der komplexen Struktur der modernen Physik untersucht, wie sie sich in den letzten 400 Jahren aufgrund der Vorstellungskraft vieler Wissenschaftler entwickelt hat. Obwohl dieses Gefüge eine sehr eindrucksvolle, wenn auch unvollständige Kohärenz hat, sollte es nicht als eine Offenbarung der letzten «Wahrheit» über die Natur angesehen werden.
Physicochemical and biological properties of poly(ethylene glycol)-coupled immunoglobulin G. Part II. Effect of molecular weight of poly(ethylene glycol) (1989)

In order to obtain a stable human immunoglobulin G (IgG) preparation for clinical use, the chemical coupling of different molecular weights of poly(ethylene glycol)s (PEGs) to IgG molecules was achieved. The abilities of PEG-coupled IgGs (PEG-IgG hybrids) to aggregate were examined when they were subjected to such physicochemical treatments as interfacial exposure, heating, lyophilization, and acid treatment. It was found that the higher the molecular weight of PEG coupled, the more stable was the PEG-IgG hybrid obtained concerning interfacial exposure and heating. The hybrid was stable against lyophilization and acid treatment and its stability was independent of the PEG molecular weight. The decrease in antigen binding ability was suppressed as much as possible by the use of a small amount of PEG of higher molecular weight. The PEG-IgG hybrids were further assessed as a stabilizer for IgG. A limited degree of PEG coupling was required for the hybrids to achieve the most efficient stabilization of IgG; the optimal PEG contents of the hybrid were greater than 20 wt% (interfacial exposure), about 5 wt% (heating), 20 wt% (lyophilization), and 10 wt% (acid treatment) for PEG 5600. It was also confirmed that the PEG-IgG hybrid was superior to PEG and human serum albumin as a stabilizer.