gebhart/patrepeval huggingface dataset:
Electrospun PLGA nanofiber scaffolds for articular cartilage reconstruction: mechanical stability, degradation and cellular responses under mechanical stimulation in vitro (2005)

We investigated the potential of a nanofiber-based poly(DL-lactide-co-glycolide) (PLGA) scaffold to be used for cartilage reconstruction. The mechanical properties of the nanofiber scaffold, degradation of the scaffold and cellular responses to the scaffold under mechanical stimulation were studied. Three different types of scaffold (lactic acid/glycolic acid content ratio = 75 : 25, 50 : 50, or a blend of 75 : 25 and 50 : 50) were tested. The tensile modulus, ultimate tensile stress and corresponding strain of the scaffolds were similar to those of skin and were slightly lower than those of human cartilage. This suggested that the nanofiber scaffold was sufficiently mechanically stable to withstand implantation and to support regenerated cartilage. The 50 : 50 PLGA scaffold was degraded faster than 75 : 25 PLGA, probably due to the higher hydrophilic glycolic acid content in the former. The nanofiber scaffold was degraded faster than a block-type scaffold that had a similar molecular weight. Therefore, degradation of the scaffold depended on the lactic acid/glycolic acid content ratio and might be controlled by mixing ratio of blend PLGA. Cellular responses were evaluated by examining toxicity, cell proliferation and extracellular matrix (ECM) formation using freshly isolated chondrocytes from porcine articular cartilage. The scaffolds were non-toxic, and cell proliferation and ECM formation in nanofiber scaffolds were superior to those in membrane-type scaffolds. Intermittent hydrostatic pressure applied to cell-seeded nanofiber scaffolds increased chondrocyte proliferation and ECM formation. In conclusion, our nanofiber-based PLGA scaffold has the potential to be used for cartilage reconstruction.
Safety and Efficacy of Durvalumab (MEDI4736), an Anti–Programmed Cell Death Ligand-1 Immune Checkpoint Inhibitor, in Patients With Advanced Urothelial Bladder Cancer (2016)

Durvalumab demonstrated a manageable safety profile and evidence of meaningful clinical activity in PD-L1-positive patients with UBC, many of whom were heavily pretreated.
Capillary zone electrophoresis with electrochemical detection (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCapillary zone electrophoresis with electrochemical detectionRoss A. Wallingford and Andrew G. EwingCite this: Anal. Chem. 1987, 59, 14, 1762–1766Publication Date (Print):July 15, 1987Publication History Published online1 May 2002Published inissue 15 July 1987https://pubs.acs.org/doi/10.1021/ac00141a005https://doi.org/10.1021/ac00141a005research-articleACS PublicationsRequest reuse permissionsArticle Views1108Altmetric-Citations313LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Sintering Time for Silica Particle Growth (2001)

The formation and growth of gas-made silica particles by co-agulation and sintering is investigated theoretically. A model for the characteristic time for silica sintering is proposed defining a minimum primary particle diameter above which macroscopic expressions are applied. The value of the minimum primary particle diameter is selected consistently with molecular dynamics simulations. The proposed characteristic sintering time is tested using a monodisperse model for aggregate dynamics by coagulation and sintering. The model predictions are compared with experimental data for silica formation and growth in premixed flames and hot wall aerosol flow reactors by oxidation of hexamethyl-disiloxane (HMDSO) and silicon-tetrachloride (SiCl4).
High resolution DNA content measurements of mammalian sperm (1982)

The high condensation and flat shape of the mammalian sperm nucleus present unique difficulties to flow cytometric measurement of DNA content. Chromatin compactness makes quantitative fluorescent staining for DNA difficult and causes a high index of refraction. The refractive index makes optical measurements sensitive to sperm head orientation. We demonstrate that the optical problems can be overcome using the commercial ICP22 epiillumination flow cytometer (Ortho Instruments, Westwood, MA) or a specially built cell orientating flow cytometer (OFCM). The design and operation of the OFCM are described. Measurements of the angular dependence of fluorescence from acriflavine stained rabbit sperm show that it is capable of orienting flat sperm with a tolerance of +/- 7 degrees. Differences in the angular dependence for the similarly shaped bull and rabbit sperm allow discrimination of these cells. We show that DNA staining with 4-6 diamidino-2-phenylindole (DAPI) or an ethidium bromide mithramycin combination allows resolution of the X and Y populations in mouse sperm. They have also been successful with sperm from the bull, ram, rabbit, and boar. Reliable results with human sperm are not obtained. The accuracy of the staining and measurement techniques are verified by the correct determination of the relative DNA content of these two populations in sperm from normal mice and those with the Cattanach [7 to X] translocation. Among the potential uses of these techniques are measurement of DNA content errors induced in sperm due to mutagen exposure, and assessment of the fractions of X and Y sperm in semen that may have one population artifically enriched.
Estimation of Articulation Angle for Tractor Semi-trailer Based on State Observer (2010)

Jackknife is an unstable state of tractor semi-trailer combination, and there is no sensor available to detect the jackknife or measure the hitch articulation angle. A state observer is designed to estimate the articulation angle, where a 3 DOF tractor semi-trailer linear model is used. The steering wheel angle sensors, lateral acceleration sensor, yaw rate sensor and axle load sensor of electronic stability control system for commercial vehicle are available here, and the signals are used in the state observer. Utilized road adhesion coefficient is estimated with lateral acceleration to correct the linear tire model. The observer is validated on a 21 DOF tractor semi-trailer nonlinear model, the simulation results shown that the estimated articulation angle is accurate in either linear area or nonlinear area of tire lateral force model.
Cellular mechanisms regulating non-haemostatic plasmin generation (2002)

A variety of proteases have the potential to degrade the extracellular matrix (ECM), thereby influencing the behaviour of cells by removing physical barriers to cell migration, altering cell-ECM interactions or releasing ECM-associated growth factors. The plasminogen activation system of serine proteases is particularly implicated in this pericellular proteolysis and is involved in pathologies ranging from cancer invasion and metastasis to fibroproliferative vascular disorders and neurodegeneration. A central mechanism for regulating plasmin generation is through the binding of the two plasminogen activators to specific cellular receptors: urokinase-type plasminogen activator to the glycolipid-anchored membrane protein uPAR, and tissue plasminogen activator to a type-II transmembrane protein recently identified on vascular smooth muscle cells. These binary complexes interact with membrane-associated plasminogen to form higher order activation complexes that greatly reduce the K(m) for plasminogen activation and, in some cases, protect the proteases from their cognate serpin inhibitors. Various other proteins that are involved in cell adhesion and migration also interact with these complexes, modulating the activity of this efficient and spatially restricted proteolytic system. Recent observations demonstrate that certain forms of the prion protein can stimulate tissue plasminogen activator-catalysed plasminogen activation, which raises the possibility that these proteases may also have a role in the pathogenesis of the transmissible spongiform encephalopathies.
Kaplan & Sadock's comprehensive textbook of psychiatry : on CD-ROM (2000)

This dynamic CD-ROM offers the complete text of the Seventh Edition of the world-renowned Comprehensive Textbook of Psychiatry - plus charts, tables, references, and illustrations you can access instantly. With this popular benchmark text on CD-ROM, you'll familiarize yourself with all DSM-IV ICD-10 disease classifications; sharpen your diagnostic skills; refresh your knowledge of new therapeutic drugs; and explore new and established approaches to psychotherapy.Precision Bar searching lets you search any way you want - by key word, topic, chapter, or image - or just browse and find information instantly. The Cross-Referencing feature directs you to sections in other chapters that are relevant to your topic of interest. A link to PubMed automatically launches your browser to PubMed's Website. A Bookmarking feature lets you personalize your program with your own annotations. The program enables you to search DSM tables, and the Table of Contents lets you quickly review and search from any screen. It is Windows / Macintosh Compatible.
Realtime ray tracing and interactive global illumination (2004)

One of the most sought-for goals in computer graphics is to generate "realism in real time". i.e. the generation of realistically looking images at realtime frame rates. Today, virtually all approaches towards realtime rendering use graphics hardware, which is based almost exclusively on triangle rasterization. Unfortunately, though this technology has seen tremendous progress over the last few years, for many applications it is currently reaching its limits in both model complexity, supported features, and achievable realism. An alternative to triangle rasterizations is the ray tracing algorithm, which is well-known for its higher flexibility, its generally higher achievable realism, and its superior scalability in both model size and compute power. However, ray tracing is also computationally demanding and thus so far is used almost exclusively for high-quality offline rendering tasks. This dissertation focuses on the question why ray tracing is likely to soon play a larger role for interactive applications, and how this scenario can be reached. To this end, we discuss the RTRT/OpenRT realtime ray tracing system, a software based ray tracing system that achieves interactive to realtime frame rates on todays commodity CPUs. In particular, we discuss the overall system design, the efficient implementation of the core ray tracing algorithms, techniques for handling dynamic scenes, an efficient parallelization framework, and an OpenGL-like low-level API. Taken together, these techniques form a complete realtime rendering engine that supports massively complex scenes, highley realistic and physically correct shading, and even physically based lighting simulation at interactive rates. In the last part of this thesis we then discuss the implications and potential of realtime ray tracing on global illumination, and how the availability of this new technology can be leveraged to finally achieve interactive global illumination - the physically correct simulation of light transport at interactive rates.
ΔNp63 intronic miR-944 is implicated in the ΔNp63-mediated induction of epidermal differentiation (2015)

ΔNp63 is required for both the proliferation and differentiation of keratinocytes, but its role in the differentiation of these cells is poorly understood. The corresponding gene, TP63, harbors the MIR944 sequence within its intron. However, the mechanism of biogenesis and the function of miR-944 are unknown. We found that miR-944 is highly expressed in keratinocytes, in a manner that is concordant with that of ΔNp63 mRNA, but the regulation of miR-944 expression under various conditions did not correspond with that of ΔNp63. Bioinformatics analysis and functional studies demonstrated that MIR944 has its own promoter. We demonstrate here that MIR944 is a target of ΔNp63. Promoter analysis revealed that the activity of the MIR944 promoter was markedly enhanced by the binding of ΔNp63, which was maintained by the supportive action of AP-2 during keratinocyte differentiation. Our results indicated that miR-944 biogenesis is dependent on ΔNp63 protein, even though it is generated from ΔNp63 mRNA-independent transcripts. We also demonstrated that miR-944 induces keratin 1 and keratin 10 expression by inhibiting ERK signaling and upregulating p53 expression. Our findings suggested that miR-944, as an intronic miRNA and a direct target of ΔNp63, contributes to the function of ΔNp63 in the induction of epidermal differentiation.
New directions in cellular therapy of cancer: a summary of the summit on cellular therapy for cancer (2012)

A summit on cellular therapy for cancer discussed and presented advances related to the use of adoptive cellular therapy for melanoma and other cancers. The summit revealed that this field is advancing rapidly. Conventional cellular therapies, such as tumor infiltrating lymphocytes (TIL), are becoming more effective and more available. Gene therapy is becoming an important tool in adoptive cell therapy. Lymphocytes are being engineered to express high affinity T cell receptors (TCRs), chimeric antibody-T cell receptors (CARs) and cytokines. T cell subsets with more naïve and stem cell-like characteristics have been shown in pre-clinical models to be more effective than unselected populations and it is now possible to reprogram T cells and to produce T cells with stem cell characteristics. In the future, combinations of adoptive transfer of T cells and specific vaccination against the cognate antigen can be envisaged to further enhance the effectiveness of these therapies.
Identification of two glycosylated components of Mycoplasma penetrans: a surface-exposed capsular polysaccharide and a glycolipid fraction (1998)

Among the wall-less mycoplasmas only a few species have been identified with a capsule at their cell surface. Mycoplasma penetrans is a recently identified mycoplasma with unique morphology, isolated from HIV-infected patients. Using transmission electron microscopy, it was found that M. penetrans is surrounded by capsular material 11 nm (strain GTU-54-6A1) to 30 nm (strain HF-2) thick, which can be stained with ruthenium red and labelled with cationized ferritin. The polysaccharide composition of this capsule was indicated by its staining with periodic acid-thiocarbohydrazide silver proteinate and the abolition of ruthenium red staining of the cell surface by neuraminidase treatment. In addition, proteinase K treatment of the M. penetrans cells resulted in removal of the capsule, suggesting that polypeptides may contribute in anchoring it to the membrane or in its stability. Two different types of glycosylated material were detected in mycoplasma extracts by SDS-PAGE and periodic acid-Schiff staining. The first component was a high-molecular-mass material, which was heat- and proteinase-K-labile and which probably constitutes the capsular polymer. The other component was a low-molecular-mass glycolipid fraction, which was proteinase-K-, heat- and EDTA-resistant. The identification of a capsule at the M. penetrans cell surface is of particular interest for a mycoplasma which has been shown to adhere to various host cells and to penetrate into their intracellular compartments. The capsule may have significance in the pathogenesis of disease associated with infection by this organism.
Activation of apoptosis signal‐regulating kinase 1 by the stress‐induced activating phosphorylation of pre‐formed oligomer (2002)

Apoptosis signal-regulating kinase 1 (ASK1) is a MAPKKK family member which activates c-Jun N-terminal kinase (JNK) and p38. In non-stressed cells, ASK1 exists as an inactive complex with the reduced form of thioredoxin. Oxidative stress such as hydrogen peroxide (H2O2) disrupts the ASK1-thioredoxin complex by oxidization of thioredoxin and thereby activates ASK1. The precise mechanism by which ASK1 is activated after its release from thioredoxin is unknown. Here we show that phosphorylation of Thr845 at the activation loop is essential for ASK1 to be activated by H2O2. ASK1 appears to form a silent homo-oligomer through its C-terminal coiled-coil region in non-stressed cells. Following H2O2 treatment, pre-existing ASK1 oligomer undergoes conformational change and creates a new interface within an oligomer, which ultimately leads to trans-autophosphorylation of Thr845. Thus, direct interaction via the coiled-coil region is required for self-scaffolding but not sufficient for activation of ASK1. Importantly, Thr845 of ASK1 can also be trans-phosphorylated by an unidentified Thr845 kinase in response to H2O2 treatment. We propose that this potential Thr845 kinase may be an ignition kinase that triggers Thr845 phosphorylation in oligomerized and activation-competent forms of ASK1.
Investigation on calibration methods for multi-axis, linear and redundant force sensors (2007)

A new method for the calibration of multi-axis, linear and redundant force sensors is presented. This new procedure, named device hyperplane characterization method, is inspired by the shape from motion method for it reduces the burden represented by the need for a huge number of force measurements, typical using least-squares methods, in order to reject errors during the calibration procedure. The proposed technique is an application of the rank theorem and achieves good noise rejection without much time consumption focusing on sensor output measurements, and reducing the effect of disturbances operating the projection of raw output data on the hyperplane to which measurements are ideally compelled to belong in the case of redundant sensors.
Basic and applied features of multicopper oxidases, CueO, bilirubin oxidase, and laccase (2007)

Multicopper oxidases (MCOs) such as CueO, bilirubin oxidase, and laccase contain four Cu centers, type 1 Cu, type II Cu, and a pair of type III Cu's in a protein molecule consisting of three domains with homologous structure to cupredoxin containing only type I Cu. Type I Cu mediates electron transfer between the substrate and the trinuclear Cu center formed by a type II Cu and a pair of type III Cu's, where the final electron acceptor O(2) is converted to H(2)O without releasing activated oxygen species. During the process, O(2) is reduced by MCOs such as lacquer laccase and bilirubin oxidase; the reaction intermediate II with a possible doubly OH(-)-bridged structure in the trinuclear Cu center has been detected. The preceding reaction intermediate I has been detected by the reaction of the lacquer laccase in a mixed valence state, at which type I Cu was cuprous and the trinuclear Cu center was fully reduced, and by the reaction of the Cys --> Ser mutant for the type I Cu site in bilirubin oxidase and CueO. An acidic amino acid residue located adjacent to the trinuclear Cu center was proved to function as a proton donor to these reaction intermediates. The substrate specificity of MCO for organic substrates is produced by the integrated effects of the shape of the substrate-binding site and the specific interaction of the substrate with the amino acid located adjacent to the His residue coordinating to the type I Cu. In contrast, the substrate specificity of the cuprous oxidase, CueO, is produced by the segment covering the Cu(I)-binding site so as to obstruct the access of organic substrates. Truncating the segment spanning helix 5 to helix 7 greatly reduced the specificity of CueO for Cu(I) and prominently enhanced the low oxidizing activity for the organic substrates, indicating the success of protein engineering to modify the substrate specificity of MCO.
Verification of Fire Models for Fire Safety System Design (1998)

Fire modeling is an essential part of risk-cost assessment. Fire models, particularly those of zone type, have flourished in recent years. The selection of a fire model as a sub- model for a fire safety system model needs further verification and review in a number of aspects. In the present study, the performances of two fire models, namely NRCC and CFAST, are evaluated with respect to accuracy, efficiency and simplicity. This study indicates that discrepancies exist between the experimental results and the predictions of both models for one type of fire scenario or another. The merits and weaknesses of the two models are identified in regard to the demands of a risk assessment system model.
Therapeutic Enhancement of IL-2 Through Molecular Design (2002)

A recombinant human IL-2 analog (rIL-2, Proleukin) is currently being evaluated for clinical benefit in HIV infected patients. It is approved for therapy of patients with metastatic melanoma and renal cell carcinoma. Treatment of cancer patients with rIL-2 results in durable responses but is associated with life-threatening toxicity, which limits its use to patients in relatively good health. Antitumor efficacy associated with rIL-2 therapy are hypothesized to be mediated by distinct types of cells that express structurally different forms of the IL-2 receptor. This hypothesis suggests that it might be possible to engineer an IL-2 variant addressing the risks associated with the therapeutic use of IL-2. In this article, we review the clinical experience with IL-2 and its analogs, the evidence that different IL-2 receptors may dissociate efficacy and toxicity, and describe the generation of a novel IL-2 variant with the potential for a superior therapeutic index.
Effect of surface composition on electronic properties of methylammonium lead iodide perovskite (2015)

Methylammonium lead iodide perovskite, CH3NH3PbI3, is one of the most promising photovoltaic materials for low-cost and clean source of energy. In this work, the first-principles calculations were carried out to investigate the different composition of CH3NH3PbI3 (001), including both methylammonium iodide terminated (MAI-T) and PbI2 terminated (PBI2-T) surfaces. The calculated surface energies show that the MAI-T is thermodynamical more stable than the PBI2-T one under the equilibrium growth condition. The electronic properties of the two types of surfaces are also different. The band gap of PBI2-T is obviously smaller than that of MAI-T due to the surface Pb states. Band gaps of MAI-T decrease with increasing thickness, while band gaps of PBI2-T are insensitive to the slab thickness. The calculated optical absorption coefficients suggest that both terminations are effective solar energy absorbers in the visible light spectrum.