gebhart/patrepeval huggingface dataset:
A dual function for adenosine 5'-triphosphate in the regulation of vascular tone. Excitatory cotransmitter with noradrenaline from perivascular nerves and locally released inhibitory intravascular agent. (1986)

A dual function for adenosine 5'-triphosphate in the regulation of vascular tone is considered. Adenosine 5'-triphosphate can cause vasodilation, acting via P2-purinoceptors located on vascular endothelial cells to release an endothelium-derived relaxing factor which diffuses to the vascular smooth muscle and induces vasodilation. The main source of intraluminal adenosine 5'-triphosphate is likely to be endothelial cells, and its release can be measured during pathophysiological conditions such as ischemia and hypoxia, in amounts likely to be sufficient to activate endothelial P2-purinoceptors. Adenosine 5'-triphosphate can also be released during intravascular platelet aggregation and from intact and damaged vascular smooth muscle cells, and so may play a role in the complex physiological mechanisms controlling local vascular tone under normoxic conditions and during vessel injury. Evidence is also presented for adenosine 5'-triphosphate acting as an excitatory cotransmitter with noradrenaline from sympathetic perivascular nerves, to cause vasoconstriction via excitatory P2-purinoceptors located on vascular smooth muscle. The postjunctional mechanical and electrical responses of a number of blood vessels following perivascular nerve stimulation contain a component that is resistant to blockade of the alpha-adrenoceptor. This nonadrenergic response is mimicked by adenosine 5'-triphosphate and can be blocked by selective desensitization of the P2-purinoceptor by alpha,beta-methylene adenosine 5'-triphosphate. Vesicular storage of adenosine 5'-triphosphate and its release from sympathetic perivascular nerves has also been demonstrated. The functional significance of adenosine 5'-triphosphate acting intraluminally as a vasodilator and extraluminally as a vasoconstrictor neuronal agent in the control of vascular tone is discussed.
Rumen Microbiome from Steers Differing in Feed Efficiency (2015)

The cattle rumen has a diverse microbial ecosystem that is essential for the host to digest plant material. Extremes in body weight (BW) gain in mice and humans have been associated with different intestinal microbial populations. The objective of this study was to characterize the microbiome of the cattle rumen among steers differing in feed efficiency. Two contemporary groups of steers (n=148 and n=197) were fed a ration (dry matter basis) of 57.35% dry-rolled corn, 30% wet distillers grain with solubles, 8% alfalfa hay, 4.25% supplement, and 0.4% urea for 63 days. Individual feed intake (FI) and BW gain were determined. Within contemporary group, the four steers within each Cartesian quadrant were sampled (n=16/group) from the bivariate distribution of average daily BW gain and average daily FI. Bacterial 16S rRNA gene amplicons were sequenced from the harvested bovine rumen fluid samples using next-generation sequencing technology. No significant changes in diversity or richness were indicated, and UniFrac principal coordinate analysis did not show any separation of microbial communities within the rumen. However, the abundances of relative microbial populations and operational taxonomic units did reveal significant differences with reference to feed efficiency groups. Bacteroidetes and Firmicutes were the dominant phyla in all ruminal groups, with significant population shifts in relevant ruminal taxa, including phyla Firmicutes and Lentisphaerae, as well as genera Succiniclasticum, Lactobacillus, Ruminococcus, and Prevotella. This study suggests the involvement of the rumen microbiome as a component influencing the efficiency of weight gain at the 16S level, which can be utilized to better understand variations in microbial ecology as well as host factors that will improve feed efficiency.
Multiple acoustic source localization using ambiguous phase differences under reverberative conditions (2004)

“Phase ambiguity” leads to confusion in computational source localization where multiple source locations are introduced from a cross-spectral phase value measured by two sensors at high frequencies, where sound wavelength is shorter than sensor interval. In this paper, a frequency domain algorithm for broadband source localization by two sensors is proposed for solving “phase ambiguity” confusion under actual conditions. Using the overlapped and averaged phase differences of the cross-spectral phases measured over the audible frequency range, multiple source azimuths are identified from each phase difference as much as possible over the azimuth range of ±90°. The frequency-independent azimuths extracted from multiple azimuths by Hough transformation provide the target source azimuths. The azimuths for the two loudspeakers can be identified simultaneously in this way from the phase differences measured over the full audible frequency range within an error of approximately 6° under reverberative conditions. By removing the numerical noise during source azimuth identification, the estimated source distribution corresponds to the diameters of the loudspeakers. When it is necessary to distinguish between near or far sound sources around the microphones, the horizontal azimuths for the sources can be precisely identified from all directions except at approximately ±90° if judgment of the front or back is given.
Principles of Microeconomics (2006)

`Else and Curwin make an effort to keep the student in touch with recent developments by including such topics as bargaining search, contestable markets and voting behaviour...it will certainly appeal to those who wish to keep economic theory accessible to as wide a range of students as possible.' Times Higher Education Supplement This clear, concise introduction to intermediate microeconomics is essential reading for students with previous knowledge of economic principles. Geared to the standard year's course in universities and polytechnics, the treatment in this text reinforces the student's understanding of familiar topics and facilitates assimilation of new material.
Morphogenesis and Tissue Engineering of Bone and Cartilage: Inductive Signals, Stem Cells, and Biomimetic Biomaterials (2000)

Morphogenesis is the developmental cascade of pattern formation, body plan establishment, and the architecture of mirror-image bilateral symmetry of many structures and asymmetry of some, culminating in the adult form. Tissue engineering is the emerging discipline of design and construction of spare parts for the human body to restore function based on principles of molecular developmental biology and morphogenesis governed by bioengineering. The three key ingredients for both morphogenesis and tissue engineering are inductive signals, responding stem cells, and the extracellular matrix. Among the many tissues in the human body, bone has considerable powers for regeneration and is a prototype model for tissue engineering based on morphogenesis. Implantation of demineralized bone matrix into subcutaneous sites results in local bone induction. This model mimics sequential limb morphogenesis and permitted the isolation of bone morphogens. Although it is traditional to study morphogenetic signals in embryos, bone morphogenetic proteins (BMPs), the inductive signals for bone, were isolated from demineralized bone matrix from adults. BMPs and related cartilage-derived morphogenetic proteins (CDMPs) initiate, promote, and maintain chondrogenesis and osteogenesis and have actions beyond bone. The symbiosis of bone inductive and conductive strategies are critical for tissue engineering, and is in turn governed by the context and biomechanics. The context is the microenvironment, consisting of extracellular matrix, which can be duplicated by biomimetic biomaterials such as collagens, hydroxyapatite, proteoglycans, and cell adhesion proteins including fibronectins. Thus, the rules of architecture for tissue engineering are an imitation of the laws of developmental biology and morphogenesis, and thus may be universal for all tissues, including bones and joints.
SYSTRAN's Pure Neural Machine Translation Systems (2016)

Since the first online demonstration of Neural Machine Translation (NMT) by LISA, NMT development has recently moved from laboratory to production systems as demonstrated by several entities announcing roll-out of NMT engines to replace their existing technologies. NMT systems have a large number of training configurations and the training process of such systems is usually very long, often a few weeks, so role of experimentation is critical and important to share. In this work, we present our approach to production-ready systems simultaneously with release of online demonstrators covering a large variety of languages (12 languages, for 32 language pairs). We explore different practical choices: an efficient and evolutive open-source framework; data preparation; network architecture; additional implemented features; tuning for production; etc. We discuss about evaluation methodology, present our first findings and we finally outline further work. Our ultimate goal is to share our expertise to build competitive production systems for "generic" translation. We aim at contributing to set up a collaborative framework to speed-up adoption of the technology, foster further research efforts and enable the delivery and adoption to/by industry of use-case specific engines integrated in real production workflows. Mastering of the technology would allow us to build translation engines suited for particular needs, outperforming current simplest/uniform systems.
Involvement of a bifunctional fatty-acyl desaturase in the biosynthesis of the silkmoth, Bombyx mori , sex pheromone (2004)

The straight-chain C(10) to C(18) unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated C(16) or C(18) fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis of these pheromone components, various combinations of limited chain-shortening and regio- and stereospecific desaturation reactions significantly contribute to the production of a vast number of the species-specific pheromone components in Lepidoptera. Biosynthesis of the silkmoth sex pheromone bombykol, (E,Z)-10,12-hexadecadien-1-ol, involves two consecutive desaturation steps, the second of which is unique in that it generates a conjugated diene system from the Delta11-monoene C(16) intermediate. In experiments designed to characterize the acyl-CoA desaturases responsible for bombykol biosynthesis, we have cloned three cDNAs encoding desaturase family members from the pheromone gland of the inbred strain of the silkmoth, Bombyx mori. Transcript analyses by RT-PCR and subsequent functional assays using a Bac-to-Bac baculovirus expression system revealed that desat1 is the only desaturase gene prominently expressed during pheromonogenesis and that its gene product, B. mori Desat1, possesses both Z11 desaturation and Delta10,12-desaturation activities. Consequently, we have concluded that B. mori Desat1 is not only a bifunctional desaturase involved in bombykol biosynthesis but that it is also the enzyme responsible for both desaturation steps.
IL-21 Is an Antitolerogenic Cytokine of the Late-Phase Alloimmune Response (2011)

IL-21 interferes with different checkpoints of the FoxP3 Treg chain in the late phase of alloimmune response and, thus, acts as an antitolerogenic cytokine. Blockade of the IL-21/IL-21R pathway could be a precondition for tolerogenic protocols in transplantation.
Separation of human plasma/serum proteins by capillary isoelectric focusing in the absence of denaturing agents (1997)

Abstract Human plasma/serum proteins were separated by capillary isoelectric focusing in the absence of denaturing agents. Proteins focused in a fused silica capillary were mobilized by replacing the catholyte sodium hydroxide to acetic acid. The performance of the separation of human plasma proteins has been examined by changing the duration of the step of isoelectric focusing, carrier ampholyte concentration, and plasma protein concentration. The separation patterns of plasma proteins were compared with those obtained by micro two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) run in the absence of denaturants in order to locate the major plasma proteins on the capillary electropherograms. Using the established electrophoretic conditions and the results of peak identification, proteins in the sera of IgG myeloma patients were analyzed by capillary isoelectric focusing. The advantages of capillary isoelectric focusing in plasma protein analysis compared with 2‐D PAGE are discussed.
Control Strategy of Speed Servo Systems Based on Deep Reinforcement Learning (2018)

We developed a novel control strategy of speed servo systems based on deep reinforcement learning. The control parameters of speed servo systems are difficult to regulate for practical applications, and problems of moment disturbance and inertia mutation occur during the operation process. A class of reinforcement learning agents for speed servo systems is designed based on the deep deterministic policy gradient algorithm. The agents are trained by a significant number of system data. After learning completion, they can automatically adjust the control parameters of servo systems and compensate for current online. Consequently, a servo system can always maintain good control performance. Numerous experiments are conducted to verify the proposed control strategy. Results show that the proposed method can achieve proportional–integral–derivative automatic tuning and effectively overcome the effects of inertia mutation and torque disturbance.
A High-strength Dacron Augmentation for Cruciate Ligament Reconstruction (1985)

Augmentation and substitution grafting was performed in 48 adult canine stifles after anterior cruciate ligament resection. Four surgical reconstructions were performed: substitution in over-the-top fashion intra-articularly with just the Dacron implant; intra-articular substitution with tibial and femoral drill holes; over-the-top substitution intra-articularly and extra-articularly with the Dacron graft augmenting the iliotibial band wraparound transfer; and two-band extra-articular Dacron substitution with the Coker modification of the MacIntosh extra-articular reconstruction without iliotibial band. Sacrifice was performed at six, 12, 24, and 36 weeks postoperation with each specimen submitted for histologic or tensile strength testing. Four canines incurred infection and were omitted from follow-up study. Examination at six and 12 weeks revealed no graft breakage, stable knees, and no evidence of degenerative change radiographically in stifles. Fibrous ingrowth was present in the intra-articular substitution and augmentation stifles. No loss of ultimate failure strength was noted during failure mode testing. The direct substitution procedure revealed some evidence of cutting-edge disruption in 24-week canines. Beveling the drill holes could eliminate this problem. Follow-up evaluation of the augmentation techniques at 24 weeks revealed increased preservation of the iliotibial band-wrapped graft by a higher degree of fibrous ingrowth. No further loss in tensile strength of the graft was noted; concurrently, no increase in tensile strength was appreciated. The use of high-tensile-strength Dacron as an augmentation to biologic transfer appears to offer significant promise as a technique for human application. The study is continuing for long-term durability of the augmentation graft.
miR-93 suppresses proliferation and colony formation of human colon cancer stem cells (2011)

Some miRNAs were differentially expressed during differentiation of SW1116csc into SW1116 cells. miR-93 may inhibit SW1116csc proliferation and colony formation.
Fully Degradable Hydrophilic Polyals for Protein Modification (2005)

Modification of proteins with hydrophilic polymers is an effective strategy for regulation of protein pharmacokinetics. However, conjugates of slowly or non-biodegradable materials, such as poly(ethylene glycol), are known to cause long-lasting cell vacuolization, in particular in renal epithelium. Conjugates of more degradable polymers, e.g., polysaccharides, have a significant risk of immunotoxicity. Polymers that combine complete degradability, long circulation in vivo, and low immuno and chemical toxicity would be most beneficial as protein conjugate components. This study explores new fully biodegradable hydrophilic polymers, hydrophilic polyals. They are nontoxic, stable at physiological conditions, and undergo proton-catalyzed hydrolysis at lysosomal pH. The model enzyme-polyal conjugates were prepared with 61-98% yield using conventional and novel conjugation techniques and retained 90-95% of specific activity. The model conjugates showed a significant prolongation of protein circulation in rodents, with a 5-fold reduction in the renal accumulation. The data suggests that hydrophilic polyals may be useful in designing protein conjugates with improved properties.
F3-Targeted Cisplatin-Hydrogel Nanoparticles as an Effective Therapeutic That Targets Both Murine and Human Ovarian Tumor Endothelial Cells In vivo (2010)

Recent studies indicate that ovarian cancer may be highly responsive to antivascular therapeutics. We have developed an antivascular tumor therapeutic using the F3 peptide to target cisplatin-loaded nanoparticles (F3-Cis-Np) to tumor vessels. We show that although F3-Cis-Np bind with high specificity to both human ovarian tumor cells and tumor endothelial cells in vitro, they only show cytotoxic activity against the tumor endothelial cells. In vivo these nanoparticles bind primarily to tumor endothelial cells. Therapeutic studies in both flank and orthotopic i.p. murine ovarian tumor models, as well as human tumor xenograft models, show rapid tumor regression with treatment. Treatment was associated with significant vascular necrosis consistent with an antivascular effect. Furthermore, treatment was active in both platinum-sensitive and platinum-resistant cell lines. Importantly, we show that F3-Cis-Np bind to human tumor endothelial cells in vitro and to human tumor vessels in vivo. Therapy targeting human vasculature in vivo with F3-Cis-Np led to near complete loss of all human tumor vessels in a murine model of human tumor vasculature. Our studies indicate that F3-targeted vascular therapeutics may be an effective treatment modality in human ovarian cancer.
A reactive behavioral system for the intelligent room (2002)

Traditional computing interfaces have drawn users into a world of windows, icons and pointers. Pervasive computing believes that human-computer interaction (HCI) should be more natural: computers should be brought into our world of human discourse. The Intelligent Room project shares this vision. We are building an Intelligent Environment (IE) to allow for more natural forms of HCI. We believe that to move in this direction, we need our IE to respond to more than just direct commands; it also needs to respond to implicit user commands, such as body language, behavior, and context, just as another human would. This thesis presents ReBa, a context-aware system to provide the IE with this type of complex behavior, in reaction to user activity.
Synthesis and optimization of digital circuits (1994)

From the Publisher:
Synthesis and Optimization of Digital Circuits offers a modern, up-to-date look at computer-aided design (CAD) of very large-scale integration (VLSI) circuits. In particular, this book covers techniques for synthesis and optimization of digital circuits at the architectural and logic levels, i.e., the generation of performance-and/or area-optimal circuits representations from models in hardware description languages. The book provides a thorough explanation of synthesis and optimization algorithms accompanied by a sound mathematical formulation and a unified notation. The text covers the following topics: modern hardware description languages (e.g., VHDL, Verilog); architectural-level synthesis of data flow and control units, including algorithms for scheduling and resource binding; combinational logic optimization algorithms for two-level and multiple-level circuits; sequential logic optimization methods; and library binding techniques, including those applicable to FPGAs.
Clinical and Endoscopic Characteristics do Not Reliably Differentiate PPI-Responsive Esophageal Eosinophilia and Eosinophilic Esophagitis in Patients Undergoing Upper Endoscopy: A Prospective Cohort Study (2013)

Esophageal eosinophilia is common among patients undergoing esophagogastroduodenoscopy for dysphagia. Although EoE was seen in nearly a quarter of patients with dysphagia, PPI-REE was almost as common, and accounted for over one-third of those with ≥15 eos/hpf. No clinical or endoscopic features independently distinguished PPI-REE from EoE before the PPI trial.
Ca2+-dependent and phospholipid-independent binding of annexin 2 and annexin 5 (2002)

Annexins are a family of homologous proteins that associate with anionic phospholipid (aPL) in the presence of Ca(2+). Evidence that the function of one annexin type may be regulated by another was recently reported in studies investigating cytomegalovirus-aPL interactions, where the fusogenic function of annexin 2 (A2) was attenuated by annexin 5 (A5). This observation suggested that A2 may bind directly to A5. In the present study, we demonstrated this interaction. The A2-A5 complex was first detected utilizing (covalently linked) fluorescein-labelled A5 (F-A5) as a reporter group. The interaction required concentrations of Ca(2+) in the millimolar range, had an apparent dissociation constant [ K (d)(app)] of 1 nM at 2 mM Ca(2+) and was independent of aPL. A2 bound comparably with F-A5 pre-equilibrated with an amount of aPL that could bind just the F-A5 or to an excess amount of aPL providing sufficient binding sites for all of F-A5 and A2. A2-A5 complex formation was corroborated in an experiment, where [(125)I]A2 associated in a Ca(2+)-dependent manner with A5 coated on to polystyrene. Surface plasmon resonance was used as a third independent method to demonstrate the binding of A2 and A5 and, furthermore, supported the conclusion that the monomeric and tetrameric forms of A2 bind equivalently to A5. Together these results demonstrate an A2-A5 interaction and provide an explanation as to how A5 inhibits the previously reported A2-dependent enhancement of virus-aPL fusion.
A potential interaction of p75 and trkA NGF receptors revealed by affinity crosslinking and immunoprecipitation (1995)

Nerve growth factor binds independently to two transmembrane receptors, the p75 neurotrophin receptor and the p140trk (trkA) tyrosine kinase receptor, which are both co-expressed in the majority of neuronal cells that respond to NGF. Previous findings have suggested that appropriate co-expression of the two receptors gives rise to high affinity NGF binding sites and increased neurotrophin responsiveness; however, evidence demonstrating a direct interaction between the two receptors in cell lines has been lacking. Here we have utilized affinity crosslinking agents with 125I-NGF to detect an association of trkA and p75 receptors in embryonic spinal cord and brain tissues enriched in the two receptors. Although multimeric complexes of trkA and p75 were not detected by affinity crosslinking, immunoprecipitation of cross-linked NGF-receptor complexes with trk-specific antibodies resulted in selective immunoprecipitation of crosslinked p75. Our results indicate that the trkA and p75 receptors can potentially interact, and that such an association may be responsible for the generation of high affinity NGF binding sites.