gebhart/patrepeval huggingface dataset:
Studies on the mechanism of lysine 2,3-aminomutase (1988)

A modified purification procedure for lysine 2,3-aminomutase from Clostridium subterminale strain SB4 has been developed. The enzyme was shown to be free of α-lysine racemase activity by use of Marfey's reagent. The isotope effect for transfer of the 3-pro-R hydrogen of α-lysine to the 2-pro-R position of β-lysine was determined to be kH/kD= 2.9 ± 0.3 by a competition experiment between L-[3,3-2H2] lysine and L-[2-2H1] lysine. No kinetic isotope effect was detected for α-deuterium substitution of α-lysine. Intermolecular transfer of deuterium was detected in two crossover experiments. Conversion of a mixture of L-[3,3-2H2] lysine and L-[4,4,5,5-2H4] lysine to β-lysine gave a product showing an enhanced M+ 5 peak in the mass spectrum. Similarly a mixture of L-[3,3-2H2] lysine +L-[UL-13C] lysine gave β-lysine having an enhanced M+ 7 peak in the mass spectrum. These results suggest that the hydrogen migration might involve a hydrogen carrier in a manner similar to the mechanisms of adenosylcobalamindependent vicinal interchange reactions. A theory that S-adenosyl-L-methionine might be acting as this hydrogen carrier was tested by co-incubating a mixture of L-[3,3-2H2]lysine and an excess of S-adenosyl-L-methionine. After incubation, the S-adenosyl-L-methionine was degraded to 5′methylthioadenosine, which was shown by 2H n.m.r. to be devoid of deuterium. The results show that S-adenosyl-L-methionine does not serve as hydrogen carrier in the reaction.
Inhibition of toll‐like receptor 4 breaks the inflammatory loop in autoimmune destructive arthritis (2007)

In the present study, we demonstrate for the first time that inhibition of TLR-4 suppresses the severity of experimental arthritis and results in lower IL-1 expression in arthritic joints. Our data suggest that TLR-4 might be a novel target in the treatment of rheumatoid arthritis.
Preliminary criteria for clinical remission in rheumatoid arthritis (1981)

A study was conducted to develop criteria for clinical remission in rheumatoid arthritis (RA). Data were provided by 35 rheumatologists on 175 RA patients considered to be in complete remission (with or without antirheumatic therapy) and 169 RA patients in partial remission or with active disease. Six criteria yielded optimal discrimination: morning stiffness absent or not exceeding 15 minutes, no fatigue, no joint pain by history, no joint tenderness, no joint or tendon sheath swelling, and no elevation of erythrocyte sedimentation rate. In this study sample, the presence of five or more of these criteria in an individual patient yielded 72% sensitivity for clinical remission and 100% specificity in discriminating RA patients with active disease. In a population sample, it is estimated that the overall accuracy of these criteria would be more than 90% in RA patients.
The characterization of micro-organisms by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (1998)

A variety of gram-positive and gram-negative intact bacterial cells have been analysed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and shown to provide fingerprint mass spectra with discrete peaks being observed over the mass range from 3 to 40 kDa. The spectra show both more peaks and peaks at a higher mass/charge ratio than have hitherto been reported for these micro-organisms and would appear to provide a profile of cellular proteinaceous material. The spectra are shown to be reproducible over variable time periods of up to three months and factors affecting reproducibility are discussed. The procedure, which requires minimal sample preparation, yields results in 30-40 minutes and allows visual identification of species- and strain-specific biomarkers for the characterization of the organisms. The importance of accurately defining sample preparation methodologies is central to the ability of the technique to generate reliable and reproducible data.
Adsorption characteristics of cellulose acetate coated charcoals (1975)

The permeability of cellulose triacetate membrane coated activated charcoal is enhanced by treatment with KOH. The adsorption data for creatinine in aqueous solution before and after deacetylation are given. In order to study the usefulness of hemoperfusion associated with dialysis for removal of uremic toxins, some experiments were performed on the adsorption by coated and deacetylated charcoal of molecules of various molecular weights (from 113 to 40,000). Although the adsorption capacity of uncoated charcoal was better, the coated material still shows good properties in the adsorption of glucagon (mol wt 3485). The results on in vitro experiments of vitamin B12 removal by coated charcoal cartridge and CDAK Model 3 dialyzer confirms the usefulness of adsorption technique toward medium molecular weight compounds.
Methods to Make Homogenous Antibody Drug Conjugates (2014)

Antibody drug conjugates (ADCs) have progressed from hypothesis to approved therapeutics in less than 30 years, and the technologies available to modify both the antibodies and the cytotoxic drugs are expanding rapidly. For reasons well reviewed previously, the field is trending strongly toward homogeneous, defined antibody conjugation. In this review we present the antibody and small molecule chemistries that are currently used and being explored to develop specific, homogenous ADCs.
Statistics and Data Analysis in Geology (1987)

From the Publisher:
This thoroughly revised edition presents important methods in the quantitative analysis of geologic data. Retains the basic arrangement of the previous edition but expands sections on probability, nonparametric statistics, and Fourier analysis. Contains revised coverage of eigenvalues and eigenvectors, and new coverage of data analysis methods, such as the semivariogram and the process of kriging.
Spectral resolution and sampling issues in Fourier-transform spectral interferometry (2000)

We investigate experimental limitations in the accuracy of Fourier-transform spectral interferometry, a widely used technique for determining the spectral phase difference between two light beams consisting of, for example, femtosecond light pulses. We demonstrate that the spectrometer’s finite spectral resolution, pixel aliasing, and frequency-interpolation error can play an important role, and we provide a new and more accurate recipe for recovering the spectral phase from the experimental data.
Design and Implementation of an Indoor Mobile Navigation System (2002)

This thesis describes the design and implementation of CricketNav, an indoor mobile navigation system using the Cricket indoor location sensing infrastructure developed at the MIT Laboratory for Computer Science as part of Project Oxygen. CricketNav navigates users to the desired destination by displaying a navigation arrow on a map. Both the direction and the position of the navigation arrow are updated in real-time as CricketNav steers the users through a dynamically computed path. To support CricketNav, we developed a modular data processing architecture for the Cricket location system, and an API for accessing location information. We implemented a least-squares position estimation algorithm in Cricket and evaluated its performance. We also developed a rich and compact representation of spatial information, and an automated process to extract this information from architectural CAD floorplans. Thesis Supervisor: Hari Balakrishnan Title: Assistant Professor
Tracking in Low Frame Rate Video: A Cascade Particle Filter with Discriminative Observers of Different Lifespans (2007)

Tracking object in low frame rate video or with abrupt motion poses two main difficulties which conventional tracking methods can barely handle: 1) poor motion continuity and increased search space; 2) fast appearance variation of target and more background clutter due to increased search space. In this paper, we address the problem from a view which integrates conventional tracking and detection, and present a temporal probabilistic combination of discriminative observers of different lifespans. Each observer is learned from different ranges of samples, with different subsets of features, to achieve varying level of discriminative power at varying cost. An efficient fusion and temporal inference is then done by a cascade particle filter which consists of multiple stages of importance sampling. Experiments show significantly improved accuracy of the proposed approach in comparison with existing tracking methods, under the condition of low frame rate data and abrupt motion of both target and camera.
A Nonhuman Primate Model of Lung Regeneration: Detergent-Mediated Decellularization and Initial In Vitro Recellularization with Mesenchymal Stem Cells (2012)

Currently, patients with end-stage lung disease are limited to lung transplantation as their only treatment option. Unfortunately, the lungs available for transplantation are few. Moreover, transplant recipients require life-long immune suppression to tolerate the transplanted lung. A promising alternative therapeutic strategy is decellularization of whole lungs, which permits the isolation of an intact scaffold comprised of innate extracellular matrix (ECM) that can theoretically be recellularized with autologous stem or progenitor cells to yield a functional lung. Nonhuman primates (NHP) provide a highly relevant preclinical model with which to assess the feasibility of recellularized lung scaffolds for human lung transplantation. Our laboratory has successfully accomplished lung decellularization and initial stem cell inoculation of the resulting ECM scaffold in an NHP model. Decellularization of normal adult rhesus macaque lungs as well as the biology of the resulting acellular matrix have been extensively characterized. Acellular NHP matrices retained the anatomical and ultrastructural properties of native lungs with minimal effect on the content, organization, and appearance of ECM components, including collagen types I and IV, laminin, fibronectin, and sulfated glycosaminoglycans (GAG), due to decellularization. Proteomics analysis showed enrichment of ECM proteins in total tissue extracts due to the removal of cells and cellular proteins by decellularization. Cellular DNA was effectively removed after decellularization (∼92% reduction), and the remaining nuclear material was found to be highly disorganized, very-low-molecular-weight fragments. Both bone marrow- and adipose-derived mesenchymal stem cells (MSC) attach to the decellularized lung matrix and can be maintained within this environment in vitro, suggesting that these cells may be promising candidates and useful tools for lung regeneration. Analysis of decellularized lung slice cultures to which MSC were seeded showed that the cells attached to the decellularized matrix, elongated, and proliferated in culture. Future investigations will focus on optimizing the recellularization of NHP lung scaffolds toward the goal of regenerating pulmonary tissue. Bringing this technology to eventual human clinical application will provide patients with an alternative therapeutic strategy as well as significantly reduce the demand for transplantable organs and patient wait-list time.
YF476 is a new potent and selective gastrin/cholecystokinin‐B receptor antagonist in vitro and in vivo (1997)

These results suggest that YF476 is an extremely potent and highly selective gastrin/CCK-B receptor antagonist, and that the gastrin/CCK-B receptor is not involved in histamine- or bethanechol-induced gastric acid secretion in dogs or rats.
In Ovo Live Imaging of Avian Embryos: Figure 1. (2010)

Vertebrate development is best studied in an intact embryo model, but a robust interface between time-lapse microscopy and in vivo embryo health and maintenance can be difficult to achieve in model systems that rely on external factors for life support. This protocol presents a system for in ovo culture and time-lapse imaging of fluorescently labeled cells within living avian embryos, using a Teflon membrane that is oxygen-permeable and liquid-impermeable. The protocol describes the Teflon membrane assembly (the assembly size can be changed to fit smaller eggs, such as those of the quail), its interface with the egg window, and the use of an upright microscope and heated chamber. The use of the system is demonstrated in chick embryos by following individual fluorescently labeled neural crest cells, a multipotent stem cell-like population that differentiates into a wide range of derivatives and travels extensively throughout the embryo. By combining in ovo culture with confocal or two-photon four-dimensional time-lapse imaging, embryo health can be maintained for up to 5 d, and neural crest cell behaviors can be visualized for long periods of time (approximately 36 h). This technique has been adapted to study somitogenesis.
HbXL99 alpha: a hemoglobin derivative that is cross-linked between the alpha subunits is useful as a blood substitute. (1987)

Under deoxygenated conditions, bis(3,5-dibromosalicyl) fumarate reacts with hemoglobin selectively to cross-link the alpha subunits between Lys-alpha 1 99 and Lys-alpha 2 99. We have characterized further the properties of this recently described hemoglobin and have demonstrated its utility as a blood substitute. The oxygen transport characteristics of the cross-linked derivative are very similar to those of whole blood. Under physiological conditions, the partial pressure of oxygen at half-saturation of hemoglobin is increased to 29 mm Hg (1 mm Hg = 133.3 kPa), compared to 12 mm Hg for hemoglobin A, fully compensating for the absence of 2,3-bisphosphoglycerate outside of the erythrocyte. The Hill coefficient is 2.9. The dependence of the oxygen affinity of HbXL99 alpha on CO2 is also identical to that of hemoglobin A. The cross-link between the alpha subunits blocks dissociation of oxyhemoglobin into alpha beta dimers and thereby prevents renal excretion of the modified hemoglobin. In the rat, the half-life of HbXL99 alpha in plasma, at a 15% volume exchange, is increased to 3.3 hr, compared to 90 min for hemoglobin A. Cross-linking HbXL99 alpha intermolecularly with bis(sulfosuccinimidyl) suberate to form predominantly a mixture of dimers and trimers further increased the half-life of the hemoglobin within the circulation by about 2-fold. The rate of autooxidation of the transfused hemoglobin was found to be markedly reduced because of the presence of an endogenous reducing system in plasma.
Analysis of Thrombi Retrieved From Cerebral Arteries of Patients With Acute Ischemic Stroke (2006)

Thromboemboli retrieved from the MCA or intracranial ICA of patients with acute ischemic stroke have similar histological components, whether derived from cardiac or arterial sources. Embolus size determines ultimate destination, those >5 mm wide likely bypassing the cerebral vessels entirely. The fibrin:platelet pattern that dominates thromboembolic structure provides a foundation for both antiplatelet and anticoagulant treatment strategies in stroke prevention.
INDUCTION OF ANTIIDIOTYPIC ANTIBODIES BY DONOR-SPECIFIC BLOOD TRANSFUSIONS (1989)

We describe a patient transfused with 200 ml of donor fresh whole blood three times at 2-week intervals. Three weeks after the last transfusion, transplantation and splenectomy were done at the same time. Splenic cells from this DST pretreated patient were fused with murine myeloma cells (X63-Ag8, 653). With DST pretreatment, various clones were developed in vivo, and finally 69 human immunoglobulin-secreting clones were obtained. Modulation of the alloantigen-specific MLR by supernatants from 69 clones showed various degrees of suppression or augmentation. The hybridoma clone 7 and clone 2, which had been secreting IgG antibody for more than 6 months, showed some degree of suppression in the alloantigen-specific MLR (mean suppression = 63%, 46% respectively). According to the result of MLR, clone 7 antibody was directed against recipient lymphocytes and clone 2 antibody was against donor lymphocytes. Immunoprecipitation was carried out by clone 7-IgG and clone 2-IgG. Clone 7-IgG specifically precipitated 1 molecule from the recipient lymphocyte with a molecular weight of 120 KD, similar to the molecular weight range reported for T cell receptors. Clone 2-IgG precipitated a 20 KD molecule from the donor lymphocyte. The data suggest that DST induces antibodies directed against the blood donor alloantigen-specific receptors on the recipient's T lymphocytes--and, at the same time, induces antibodies against donor lymphocyte antigens. These antibodies may be essential to prolongation of kidney allograft survival following DST.
Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature (1993)

The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.
Document filtering with inference networks (1996)

Article Document filtering with inference networks Share on Author: Jamie Callan Computer Science Department, University of Massachusetts, Amherst, MA Computer Science Department, University of Massachusetts, Amherst, MAView Profile Authors Info & Claims SIGIR '96: Proceedings of the 19th annual international ACM SIGIR conference on Research and development in information retrievalAugust 1996 Pages 262–269https://doi.org/10.1145/243199.243273Online:18 August 1996Publication History 64citation457DownloadsMetricsTotal Citations64Total Downloads457Last 12 Months3Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Reduced Expression of PTPRD Correlates with Poor Prognosis in Gastric Adenocarcinoma (2014)

These results indicated that PTPRD is a candidate tumour suppressor in gastric cancer. Thus, PTPRD may play an important role in gastric tumorigenesis and serve as a valuable prognostic marker of gastric adenocarcinoma.
Three-Dimensional Primary Hepatocyte Culture in Synthetic Self-Assembling Peptide Hydrogel (2008)

Drug metabolism studies and liver tissue engineering necessitate stable hepatocyte cultures that express liver functions for a minimum of 4 days to 3 weeks. Current techniques, using different biomaterials and geometries, that maintain hepatocellular function in vitro exhibit a low cell density and functional capacity per unit volume. Herein we investigated a well-defined synthetic peptide that can self-assemble into three-dimensional interweaving nanofiber scaffolds to form a hydrogel, PuraMatrix, as a substrate for hepatocyte culture. Freshly isolated primary rat hepatocytes attached, migrated, and formed spheroids within 3 days after seeding on PuraMatrix. Hepatocytes expressed the apical membrane marker dipeptidyl peptidase IV at cell-cell contacts. Compared to the collagen sandwich, albumin and urea secretion on PuraMatrix were higher for the first week, and cytochrome P450IA1 activity was higher throughout the culture period. Mitochondrial membrane potential 1 day after seeding was higher on PuraMatrix than in the collagen sandwich, suggesting better preservation of the metabolic machinery. PuraMatrix and Matrigel showed similar albumin and urea production. PuraMatrix is an attractive system for generating hepatocyte spheroids that quickly restore liver functions after seeding. This system is also amenable to scale-up, which makes it suitable for in vitro toxicity, hepatocyte transplantation, and bioartificial liver development studies.
DAIRY '96, PART II: CHANGES IN THE U.S. DAIRY INDUSTRY: 1991-1996 (1996)

The NAHMS Dairy '96 Study was designed to provide both participants and the industry with information on the nation's dairy animal population for education and research. This report contains demographic changes of the U.S. and world dairy industry from a historical perspective from data provided by the National Agricultural Statistics Service (NASS), Census of Agriculture, and Foreign Agriculture Service. Results of two NAHMS national studies overview changes in the U.S. dairy industry in the 5-year period of 1991 to 1996. The 1991 National Dairy Heifer Evaluation Project included herds of 30 or more milk cows and heifer-rearing operations in 28 states representing 83 percent of U.S. milk cows. NAHMS Dairy '96 described dairy production for operations with one or more milk cows in 20 states representing 83 percent of the nation's milk cows. This report provides national estimates of animal health and health management practices for comparable populations from both studies. Contact for this paper: Steven Ott
Selective formation of HCO2− and C2O42− in electrochemical reduction of CO2 catalyzed by mono- and di-nuclear ruthenium complexes (1998)

Electrochemical reduction of carbon dioxide catalyzed by mono- and di-nuclear ruthenium complexes produced HCO 2H with trace amounts of CO and C 2O 4 2– in the presence and absence of H 2O, respectively, in MeCN.
Assessment of the risk of vertebral fracture in menopausal women. (1987)

The decision to institute prophylaxis in women with menopausal osteopenia is hampered by the absence of quantitative criteria for appraising the risk of fracture in the individual. We have developed standards for assessing the risk of fracture by relating the prevalence of atraumatic vertebral compression fractures to bone density in sixty-five menopausal women, forty-nine to ninety-two years old. To define the upper limit of the spectrum of bone density, we also studied thirty-one young women, seventeen to twenty-two years old. The density of trabecular bone in a vertebral body was determined by quantitative computed tomography and expressed in terms of milligrams per milliliter of dipotassium hydrogen phosphate. Twenty-five of the menopausal women exhibited at least one fracture (range, one to six fractures), and forty had no fracture. The bone density ranged from -9 to sixty-nine milligrams per milliliter in those with fractures and from twelve to 122 milligrams per milliliter in those without a fracture. The densities in the young women averaged 173 milligrams per milliliter and ranged from ninety-five to 248 milligrams per milliliter. The percentage of subjects with fractures increased as the bone density decreased. It was zero per cent in women with a density of seventy milligrams per milliliter or more, 38 per cent in women with a density between fifty and less than seventy milligrams per milliliter, 71 per cent in those with a density between thirty and less than fifty milligrams per milliliter, and 82 per cent in women with a density of less than thirty milligrams per milliliter.(ABSTRACT TRUNCATED AT 250 WORDS)
Using Microsoft Excel 97 (1997)

Using Microsoft Excel 97 , Using Microsoft Excel 97 , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی