gebhart/patrepeval huggingface dataset:
Combined genetic profiles of components and regulators of the vitamin K-dependent γ-carboxylation system affect individual sensitivity to warfarin (2006)

We examined the influence of combined genotypes on interindividual variability in warfarin dose-response. In 100 anticoagulated patients we quantified the effects of polymorphisms in: CYP2C9, VKORC1, calumenin (CALU), gamma-glutamyl carboxylase (GGCX) and microsomal epoxide hydrolase (EPHX1) on warfarin dose requirements. The G(1542)C VKORC1 polymorphism was associated with decreased warfarin doses in the hetero- and homozygous mutant patients (21% and 50% lower, respectively; p < 0.0001). Warfarin daily dose was predominantly determined by VKORC1 and CYP2C9 genotypes (partial r(2) = 0.21; 0.20, respectively). Together with age and body weight, these two genotypes explained 63% of the dose variance. A single patient, homozygous for G(11)A CALU mutant allele, required an exceptionally high warfarin dose (20 mg/day) and the prevalence of heterozygous (11)A allele carriers in the upper 10(th) dose percentile was significantly higher (0.27 vs. 0.18, p < 0.02). Combined genotype analysis revealed that CYP2C9 andVKORC1 wild type and CALU mutant patients required the highest warfarin doses (7.8 +/- 1.5mg/day; n = 9) as compared to the CYP2C9 and VKORC1 mutant and CALU wild type genotypes (2.8 +/- 0.3 mg/day; n = 18; p < 0.01). The odds ratio for doses <3mg/day was 5.9 (1.9-18.4) for this genotype. Compound genetic profiles comprising VKORC1, CALU and CYP2C9 improve categorization of individual warfarin dose requirements in more than 25% of patients at steady-state anticoagulation.
A Study of Filter Bank Smoothing in MFCC Features for Recognition of Children's Speech (2007)

In this paper, we study the effect of filter bank smoothing on the recognition performance of children's speech. Filter bank smoothing of spectra is done during the computation of the Mel filter bank cepstral coefficients (MFCCs). We study the effect of smoothing both for the case when there is vocal-tract length normalization (VTLN) as well as for the case when there is no VTLN. The results from our experiments indicate that unlike conventional VTLN implementation, it is better not to scale the bandwidths of the filters during VTLN - only the filter center frequencies need be scaled. Our interpretation of the above result is that while the formant center frequencies may approximately scale between speakers, the formant bandwidths do not change significantly. Therefore, the scaling of filter bandwidths by a warp-factor during conventional VTLN results in differences in spectral smoothing leading to degradation in recognition performance. Similarly, results from our experiments indicate that for telephone-based speech when there is no normalization it is better to use uniform-bandwidth filters instead of the constant- like filters that are used in the computation of conventional MFCC. Our interpretation is that with constant- filters there is excessive spectral smoothing at higher frequencies which leads to degradation in performance for children's speech. However, the use of constant- filters during VTLN does not create any additional performance degradation. As we will show, during VTLN it is only important that the filter bandwidths are not scaled irrespective of whether we use constant- or uniform-bandwidth filters. With our proposed changes in the filter bank implementation we get comparable performance for adults and about 6% improvement for children both for the case of using VTLN as well as the for the case of not using VTLN on a telephone-based digit recognition task.
Efficacy and Safety of Prescription Omega-3 Fatty Acids for the Prevention of Recurrent Symptomatic Atrial Fibrillation (2010)

clinicaltrials.gov Identifier: NCT00402363.
Non-fullerene acceptors for organic photovoltaics: an emerging horizon (2014)

Although fullerenes and their derivatives, such as PCBM, have been the dominant electron-acceptor materials in organic photovoltaic cells (OPVs), they suffer from some disadvantages, such as weak absorption in the visible spectral region, limited spectral breadth and difficulty in variably tuning the band gap. It is necessary to explore non-fullerene electron acceptors that will not only retain the favorable electron-accepting and transporting properties of fullerenes but also overcome their insufficiencies. After a decade of mediocrity, non-fullerene acceptors are undergoing rapid development and are emerging as a hot area of focus in the field of organic semiconductors. Solution-processed bulk heterojunction (BHJ) OPVs based on non-fullerene acceptors have shown encouraging power conversion efficiencies of over 4%. This article reviews recent developments in several classes of solution-processable non-fullerene acceptors for BHJ OPVs. The remaining problems and challenges along with the key research directions in the near future are discussed.
Non-surgical treatment of hepatocarcinoma (1993)

Hepatocellular carcinoma (HCC) is one of the most common tumors affecting man. It is the general feeling that only hepatectomy can give a chance for cure. However, less than 20% of patients can be resected, and other treatment modalities are required. Systemic (chemotherapy, hormonotherapy, immunotherapy) and loco-regional (intratumoral injection of alcohol, intra-arterial chemotherapy embolization, internal radiotherapy) approaches have been developed. In view of the small number of patients, tumor and patient heterogeneity, and difficulties in assessing tumor response, the real place of these treatments is difficult to evaluate. A review of the literature suggests that embolization with Gelfoam, even when given without chemotherapy, has an effect on response rate and on survival, and could be considered, at the present time, as the most attractive treatment in non-operable HCC. Chemotherapy seems effective only if combined with embolization. When administered alone by the systemic or the intra-arterial hepatic route, no clinically significant activity can be found. Unexpectedly, Lipiodol by itself seems inactive, and the co-administration of chemotherapy does not improve activity. Other approaches such as intratumoral injection of alcohol, immunotherapy, hormonotherapy, and radioimmunotherapy are still experimental, and well-designed studies are needed to identify their role.
Endotoxin-responsive sequences control cachectin/tumor necrosis factor biosynthesis at the translational level. (1990)

The biosynthesis of cachectin/TNF is largely regulated at a post-transcriptional level. Bacterial endotoxin, which strongly induces cachectin/TNF production, thus seems to elicit at least some of its effects by altering the macrophage cytoplasmic milieu. It has previously been shown that the 3'-untranslated TTATTTAT element present in numerous cytokines and proto-oncogenes is capable of repressing the translation of mRNA molecules in which it is represented. Using constructs in which the CAT coding sequence is followed by varying segments of the cachectin/TNF 3'-untranslated region, we now demonstrate that downstream sequences present in the cachectin/TNF mRNA are sufficient to mediate greater than 200-fold induction of CAT synthesis in response to activation by endotoxin. Induction of CAT activity is not attributable to a change in cytoplasmic mRNA concentration, but to a marked enhancement of translational efficiency. The response to endotoxin represents "derepression," and is conferred chiefly by the translationally repressive TTATTTAT element, acting in concert with essential flanking sequences.
Efficacy of low-dose spironolactone in subjects with resistant hypertension (2003)

We conclude that low-dose spironolactone provides significant additive BP reduction in African American and white subjects with resistant hypertension with and without primary aldosteronism.
Comment on “Time-resolved laser induced harpoon reactions’’ (1997)

Time resolved studies of laser induced harpoon reactions in clusters and in liquid phase have succeeded in separating coherent and sequential two-photon channels, and have provided direct observations of bimolecular chemistry through control of products. The same cannot be said about the gas phase experiments on these systems.
Effect of Nutrient Conditions on the Growth of Botryococcus braunii (2003)

The effect of nutrient conditions, including incarbon sources, nitrate and phosphate, on the growth of hydrocarbon-rich microalga Botryococcus braunii was investigated in shake flask culture. The complement of NaHCO3 and CO2 made growth rate of Botryococcus braunii increase from 0.0535 d-1 to 0.0589 d-1, 0.0949 d-1 respectively, generation time reduced from 5.6 d to 5.1 d, 3.2 d respectively, maximum oxygen evolution rate improved 2.3 and 5 times respectively by comparison with control cultures. The growth rate of Botryococcus braunii went up and the exponential phase lengthened with the increase of the initial concentration of KNO3 within some scope. K2HPO4 in the range of 0~160 mg/L could improve the growth rate of Botryococcus braunii significantly. In addition, the role of NaF was examined. NaF promoted the photosynthesis and respiration activity. Its suitable concentration was 0.84 mg/L.
The extracellular glycoprotein SPARC interacts with platelet-derived growth factor (PDGF)-AB and -BB and inhibits the binding of PDGF to its receptors. (1992)

Interactions among growth factors, cells, and extracellular matrix are critical to the regulation of directed cell migration and proliferation associated with development, wound healing, and pathologic processes. Here we report the association of PDGF-AB and -BB, but not PDGF-AA, with the extracellular glycoprotein SPARC. Complexes of SPARC and 125I-labeled PDGF-BB or -AB were specifically immunoprecipitated by anti-SPARC immunoglobulins. 125I-PDGF-BB and -AB also bound specifically to SPARC that was immobilized on microtiter wells or bound to nitrocellulose after transfer from SDS/polyacrylamide gels. The binding of PDGF-BB to SPARC was pH-dependent; significant binding was detectable only above pH 6.6. The interaction of SPARC with specific dimeric forms of PDGF affected the activity of this mitogen. SPARC inhibited the binding of PDGF-BB and PDGF-AB, but not PDGF-AA, to human dermal fibroblasts in a dose-dependent manner. The expression of SPARC and PDGF was minimal in most normal adult tissues but was increased after injury. Enhanced expression of both PDGF-B chain and SPARC was seen in advanced lesions of atherosclerosis. We suggest that the coordinate expression of SPARC and PDGF-B-containing dimers following vascular injury may regulate the activity of specific dimeric forms of PDGF in vivo.
Characterization of Tn917 insertion mutants of Staphylococcus epidermidis affected in biofilm formation (1996)

Biofilm formation is thought to result from the concerted action of primary attachment to a specific surface and accumulation in multilayered cell clusters. Here we describe the isolation and characterization of transposon (Tn917) mutants of Staphylococcus epidermidis O-47 which were biofilm negative in the polystyrene microtiter plate assay. Among 5,000 Tn917 insertion mutants, 4 biofilm-negative mutants were isolated. Each mutant carried one copy of Tn917. The mutants were divided into two phenotypic classes: class A (mut1 and mut1a) and class B (mut2 and mut2a). Mutants of phenotypic class A lacked four cell surface proteins, were less hydrophobic, and were affected in primary attachment to polystyrene, but were still able to form multilayered cell clusters. They were able to form a biofilm on a glass surface, a trait that was even more pronounced than in the wild-type stain O-47. Loss of several surface proteins might have led to the reduced surface hydrophilic structures, thus favoring primary attachment to a glass surface and leading to subsequent biofilm formation. Mutants of phenotype class B were able to attach to polystyrene but were unable to form multilayered cell clusters, had unchanged cell surface proteins and hydrophobicity, and were unable to form a biofilm on a glass surface, mut1 and mut2 could be complemented by wild-type DNA fragments containing the Tn917 insertion sites of mut1 and mut2, respectively. The complemented biofilm-positive clone mut1 (pRC20) produced a 60-kDa protein which is postulated to function as the adhesin for binding to plastic. The traits of binding to polystyrene and the ability to form multilayered cell clusters are phenotypically and genetically distinct.
Antioxidant Activity of Lignin Phenolic Compounds Extracted from Kraft and Sulphite Black Liquors (2010)

The antioxidant activity of the phenolic compounds present in industrial black liquors obtained from the two cooking processes (kraft and sulphite) used in Portugal to produce Eucalyptus globulus pulp was evaluated. The black liquors treated at several pH values were extracted with ethyl acetate. Phenolic fractions were further separated by liquid chromatography of the crude extracts of kraft liquor at pH = 6 and sulphite liquor at the original pH. Total phenolic content was determined in terms of gallic acid equivalents (Folin-Ciocalteu colorimetric method), and the antioxidant activity in the crude extracts at several pH values and in the separated fractions was measured using the DPPH test for radical scavenging capacity. The total phenolic content of crude extracts and separated fractions ranged from 92.7 to 181.6 and from 91.6 to 1,099.6 mg GAE/g, respectively, while the antioxidant activity index (AAI) ranged from 2.20 to 3.41 and from 2.21 to 11.47 respectively, showing very strong antioxidant activity in all studied cases. The fractions separated by column chromatography were submitted to mass spectrometry analysis and the results were compared to others in the literature of natural products, mainly from Eucalyptus, and the characteristic bands of functional groups were identified by ¹H-NMR and FTIR. These methods allowed the identification of 17 phenolic compounds.
Isolation of cDNA clones for human complement component C2. (1984)

Two cDNA clones for complement component C2 have been isolated from a high-complexity human liver cDNA library by using a mixture of 64 synthetic oligonucleotides as a probe. The 400-base-pair insert of pC201 codes for a region containing the active site serine residue and the secondary substrate binding pocket of the serine protease. This part of C2 is 34% homologous to the corresponding region of the related serine protease factor B and additional similarity is evident from a number of conservative amino acid replacements in this region. The insert of pC201 was used as a specific probe in RNA transfer analysis to determine the size of the C2 mRNA as approximately equal to 2.9 kilobases. Southern blot analysis of genomic DNA of unrelated individuals identified a single C2 locus and showed no cross-hybridization with the factor B locus.
Interface Development for a Child's Video Conferencing Robot (2000)

The PEBBLES communication system, designed to link children in the hospital with their regular classrooms, has progressed through three major development and evaluation phases. Many of the design and human factors issues (e.g., daily curriculum planning and co-ordination between two teachers in distant locations, and the need for an appropriate attention device) discovered through this process are unique from other video-mediated communication systems because PEBBLES must support both academic and social tasks with children as users. While technical limitations such as bandwidth and audio fidelity remain as problems to solve with PEBBLES, the system has been successful in providing students with a high level of telepresence in their classrooms.
RENAL AUTOTRANSPLANTATION FOR THE LOIN PAIN-HEMATURIA SYNDROME: LONG-TERM FOLLOWUP OF 26 CASES (1998)

Renal autotransplantation results in durable narcotic-free favorable results in the majority of meticulously screened loin pain-hematuria syndrome patients. Although some failures, which usually occur within 2 years after surgery, can be expected, autotransplantation is justified as a nephron sparing denervation therapy for select loin pain-hematuria syndrome patients.
A cell biological perspective on mitochondrial dysfunction in Parkinson disease and other neurodegenerative diseases (2007)

Dysfunction of mitochondria is frequently proposed to be involved in neurodegenerative disease. Deficiencies in energy supply, free radical generation, Ca(2+) buffering or control of apoptosis, could all theoretically contribute to progressive decline of the central nervous system. Parkinson disease illustrates how mutations in very different genes finally impinge directly or indirectly on mitochondrial function, causing subtle but finally fatal dysfunction of dopaminergic neurons. Neurons in general appear more sensitive than other cells to mutations in genes encoding mitochondrial proteins. Particularly interesting are mutations in genes such as Opa1, Mfn1 and Dnm1l, whose products are involved in the dynamic morphological alterations and subcellular trafficking of mitochondria. These indicate that mitochondrial dynamics are especially important for the long-term maintenance of the nervous system. The emerging evidence clearly demonstrates the crucial role of specific mitochondrial functions in maintaining neuronal circuit integrity.
Rosacea-Like Demodicosis Induced by Topical Pimecrolimus: Immunohistochemical Evaluation of Inflammatory Infiltrate (2009)

A B S T R A C T We present a 48-year-old female patient who developed rosacea-like demodicosis after seven days of topical application of 1% pimecrolimus cream. The inflammatory infiltrate in the lesions was mainly composed of T-cells with a CD4+/CD8+ ratio = 0.33; B-cells (CD20+) and NK-cells (CD56+) were not detected. Large numbers of (CD68+) histiocytes were demonstrated, whereas the number of Langerhans cells (CD1a+) revealed no significant alteration. The reduced CD4+:CD8+ ratio may have led to a modification of follicular microenvironment favouring the excessive proliferation of Demodex mites, who triggered a presently unknown sequence of events leading to the development of pimecrolimus-induced rosacea-like demodicosis.
Cleaning of inorganic membranes after whey and milk ultrafiltration (1991)

Cleaning of an inorganic ultrafiltration membrane has been quantified through hydraulic, physicochemical, and spectroscopic (infrared and x-photoelectron spectroscopy) analyses. An efficient cleaning sequence of nitric acid followed by sodium hypochlorite has been proposed for cleaning of defatted whey protein concentrate and milk ultrafiltration membranes. The influence of reversed sequence and time reduction are discussed together with the action of both cleaning chemicals. In spite of residual fouling left after every cleaning sequence studied, hydraulic cleanliness of the membrane was achieved, particularly after the standard procedure.
Simplified CLARITY for visualizing immunofluorescence labeling in the developing rat brain (2016)

CLARITY is an innovative technological ad- vance in which intact biological tissue is transformed into a ''nanoporous hydrogel-hybridized form'' (Chung et al. 2013; Chung and Deisseroth 2013) with markedly improved che- mical and optical accessibility, permitting fluorescent visu- alization and extraction of high-resolution structural data from mm-thick blocks of tissue. CLARITY affords an ex- cellent but as yet unexploited opportunity to visualize the growth and maturation of phenotypically identified neurons and axonal processes in the developing brain. This brief report describes a moderately revised, simplified, and less expensive CLARITY protocol that effectively reveals the structure of chemically identified neurons in whole neonatal/ juvenile rat brains and tissue slabs. Rats (postnatal day (P)0-24) were transcardially perfused with one of two fixative/hydrogel solutions, followed by hydrogel polymer- ization to generate brain hybrids. Whole brain hybrids or 2.0-mm-thick coronal slabs were passively cleared of lipid and then processed for dual immunofluorescence labeling, including labeling using tyramide signal amplification. After refractive index matching using 2,20-Thiodiethanol (60 % solution), a Leica confocal microscope equipped with a CLARITY objective was used to view the hypothalamus in whole brain hybrids or slabs. Collected image stacks re- vealed the distribution and three-dimensional structure of hypothalamic pro-oxyphysin (oxytocin)-, neuropeptide Y-, glucagon-like peptide-1-, and tyrosine hydroxylase- immunopositive neurons and processes within large tissue volumes. Outstanding structural preservation and immuno- labeling quality demonstrates the efficacy of this approach for interrogating chemically defined neural circuits as they develop in postnatal rodent brain.
National Trends in the Treatment of Attention Deficit Hyperactivity Disorder (2003)

During the decade, there was a marked and broad expansion in access to treatment of children with ADHD but a decline in intensity of treatment, as measured by number of visits. These changes occurred during a period of expanding access to special education services, growth of managed behavioral healthcare, and increased public acceptance of effective psychotropic medications.
Control of gene expression in trypanosomes (1995)

Trypanosomes are protozoan agents of major parasitic diseases such as Chagas' disease in South America and sleeping sickness of humans and nagana disease of cattle in Africa. They are transmitted to mammalian hosts by specific insect vectors. Their life cycle consists of a succession of differentiation and growth phases requiring regulated gene expression to adapt to the changing extracellular environment. Typical of such stage-specific expression is that of the major surface antigens of Trypanosoma brucei, procyclin in the procyclic (insect) form and the variant surface glycoprotein (VSG) in the bloodstream (mammalian) form. In trypanosomes, the regulation of gene expression is effected mainly at posttranscriptional levels, since primary transcription of most of the genes occurs in long polycistronic units and is constitutive. The transcripts are processed by transsplicing and polyadenylation under the influence of intergenic polypyrimidine tracts. These events show some developmental regulation. Untranslated sequences of the mRNAs seem to play a prominent role in the stage-specific control of individual gene expression, through a modulation of mRNA abundance. The VSG and procyclin transcription units exhibit particular features that are probably related to the need for a high level of expression. The promoters and RNA polymerase driving the expression of these units resemble those of the ribosomal genes. Their mutually exclusive expression is ensured by controls operating at several levels, including RNA elongation. Antigenic variation in the bloodstream is achieved through DNA rearrangements or alternative activation of the telomeric VSG gene expression sites. Recent discoveries, such as the existence of a novel nucleotide in telomeric DNA and the generation of point mutations in VSG genes, have shed new light on the mechanisms and consequences of antigenic variation.