gebhart/patrepeval huggingface dataset:
Effect of omega-6 lipid-rich borage oil feeding on immune function in healthy volunteers (1997)

Conference Article| May 01 1997 Effect of omega-6 lipid-rich borage oil feeding on immune function in healthy volunteers BENJAMIN A.C. FISHER; BENJAMIN A.C. FISHER *Department of Immunology, United Medical and Dental Schools of Guy's and St Thomas', The Rayne Institute, St Thomas' Hospital, London SE1 7EH U.K. Search for other works by this author on: This Site PubMed Google Scholar LAURENCE S. HARBIGE LAURENCE S. HARBIGE *Department of Immunology, United Medical and Dental Schools of Guy's and St Thomas', The Rayne Institute, St Thomas' Hospital, London SE1 7EH U.K.#Physiological Sciences Research Group, School of Chemical and Life Sciences, University of Greenwich, London SE18 6PF U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1997) 25 (2): 343S. https://doi.org/10.1042/bst025343s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation BENJAMIN A.C. FISHER, LAURENCE S. HARBIGE; Effect of omega-6 lipid-rich borage oil feeding on immune function in healthy volunteers. Biochem Soc Trans 1 May 1997; 25 (2): 343S. doi: https://doi.org/10.1042/bst025343s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: EL1SA, enzyme-linked immunosorbent assay, IL, interleukin, IFNγ, interferon-gamma, LPS, lipopolysaccharide, PHA, phytohaemagglutinin, TGFβ, transforming growth factor beta, TNFα, tumour necrosis factor alpha This content is only available as a PDF. © 1997 Biochemical Society1997 Article PDF first page preview Close Modal You do not currently have access to this content.
Lack of β-catenin affects mouse development at gastrulation (1995)

Molecular analysis of the cadherin-catenin complex elucidated the central role of beta-catenin in this adhesion complex, as it binds to the cytoplasmic domain of E-cadherin and to alpha-catenin. beta-Catenin may also function in signalling pathways, given its homology to the gene product of the Drosophila segment polarity gene armadillo, which is known to be involved in the wingless signalling cascade. To study the function of beta-catenin during mouse development, gene knock-out experiments were performed in embryonic stem cells and transgenic mice were generated. beta-Catenin null-mutant embryos formed blastocysts, implanted and developed into egg-cylinder-stage embryos. At day 7 post coitum, the development of the embryonic ectoderm was affected in mutant embryos. Cells detached from the ectodermal cell layer and were dispersed into the proamniotic cavity. No mesoderm formation was observed in mutant embryos. The development of extraembryonic structures appeared less dramatically or not at all affected. Our results demonstrate that, although beta-catenin is expressed rather ubiquitously, it is specifically required in the ectodermal cell layer.
Conformational and epitope mapping of herpes‐simplex‐virus type‐1 thymidine kinase using synthetic peptide segments (1991)

Adjacent peptide segments covering the complete sequence of herpes-simplex-virus type-1 thymidine kinase (HSV1-TK) of 376 amino acids were synthesized in order to experimentally verify the three-dimensional structure of the HSV1-TK active site, which was previously determined by molecular modeling. 26 peptides have been prepared by multiple solid-phase synthesis using the 9-fluorenylmethoxycarbonyl strategy. The purified peptides were linked covalently to bovine serum albumin. The peptide/ELISA of the synthesized bovine-serum-albumin conjugates using polyclonal rabbit anti(HSV1-TK)serum resulted in ten epitopes, which correlate excellently with the computer-proposed active site of HSV1-TK. CD spectra of the HSV1-TK peptides were recorded in trifluoroethanol/water (9:1 by vol.) An eigenvalue method based on CD spectra of 15 well known protein structures was used to calculate the relative percentage of secondary structures from the CD data. The computer model of the HSV1-TK showed full conformity with the folding pattern determined by CD of the synthetic peptide segments. Therefore, conformational peptide mapping with CD-based secondary structures combined with epitope mapping from the peptide/ELISA is an efficient and reliable method to support computer-aided protein design.
Interfacing incompatible protocols using interface process generation (1995)

During system design, one or more portions of the system may be implemented with standard components that have a fixed pin structure and communication protocol. This paper described a new technique, interface process generation, for interfacing standard components that have incompatible protocols. Given an HDL description of the two protocols, we present a method to generate an interface process that allows the two protocols to communicate with each other.
Autoimmune Hyperchylomicronemia (1989)

AMONG the hyperchylomicronemias, familial deficiencies of lipoprotein lipase (LPL) and of apolipoprotein C-II are rare variants in which the underlying biochemical defects are relatively well defined. The diseases are characterized by a deficiency of LPL, which is the key enzyme in the hydrolysis of triglyceride-rich lipoproteins, or a defect in apolipoprotein C-II, a cofactor of LPL.1 2 3 4 Hyperchylomicronemia may also be a primary characteristic of type V familial hyperlipoproteinemia,5 or it may be secondary to a variety of conditions, such as diabetes mellitus, alcoholism, chronic renal failure, or hypothyroidism.6 7 8 9 10 11 The mechanism of secondary hyperchylomicronemia has not been fully elucidated.We describe . . .
Offset curve deformation from skeletal animation (2008)

Skin deformation based on an underlying skeleton is a critical part of the modern animation pipeline. Linear blend skinning is fast but suffers volume loss at a bend and “candy wrapper” pinching under twist. More complex methods using dual quaternions [Kavan et al. 2007] and bind-point offset from intermediate curves [Singh and Fiume 1998] are nearly as fast and reduce these artifacts, but dual quaternions are limited only to rigid transformations and bind-point techniques continue to suffer from offset distortion, which increases with distance from the bind point, causing uneven stretching and self-intersection on the inside of a bend when offsets exceed the radius of curvature.
A Beam-splitting Device for Use with Fiber-coupled Laser Light Sources for Photodynamic Therapy¶ (2002)

A device that divides light into eight, four or two beams of equivalent power with only minor total power loss was designed, built and tested. The apparatus accepts light from a 200 microm diameter, 0.16 numerical aperture, silica-silica multimode optical fiber connected to one of several laser light sources for photodynamic therapy (PDT) of cancer. The incorporation of a variable iris diaphragm into the optical couplers allows the power of the beams to be independently set. Each of the beams can be coupled to a 400 or 600 microm diameter optical fiber to deliver the therapeutic light to the patient. This device is used in our institute for PDT of patients with either numerous small malignant tumors or single tumors with large surface area.
Design of substrate-site-directed irreversible inhibitors of adenosine 5'-phosphate aminohydrolase. Effect of substrate substituents on affinity for the substrate site (1976)

Derivatives of adenosine 5'-phosphate (AMP) have been synthesized in which the phosphoester (POCH2) grouping of AMP is replaced by PCH(R)CH2 where R is OC(O)Me, CH2NHCOMe, CH2NHCOEt, and CH2NHCOOR' (R' = Me, Et, and Pr). The 2',3'-O-isopropylidene and 2', 3'-di-O-acetyl derivatives of AMP were also prepared. All compounds were competitive inhibitors of rabbit muscle AMP aminohydrolase with enzyme-inhibitor dissociation constants (Ki values) of 330, 20, 17, 19, 16, 14, 260, and 105 muM, respectively. All compounds were substrates except those in which R was CH2NHCOEt and CH2NHCOOR' (R'=Me, Et, and Pr). The previously described allo and talo epimers of 5'-C-acetylaminomethyl-AMP and the allo epimer of 5'-C-propionylaminomethyl-AMP were substrates and competitive inhibitors with Ki values of 18, 47, and 42 muM, respectively. The talo epimer of 5'-C-propionylaminomethyl-AMP was not a substrate and was a noncompetitive inhibitor, Ki
An Empirical Comparison of Four Generic Health Status Measures (1997)

None of the instruments performed uniformly as "best" or "worst." Purely on the basis of the results of the psychometric analyses, the SF-36 appeared to be the most suitable measure of health status in this relatively healthy population. In general, the choice of the most suitable instrument for generic health status assessment in a particular study should be guided by the special features of each candidate instrument under consideration.
Interleukin-9 over-expression and T helper 9 polarization in systemic sclerosis patients (2017)

T helper 9 (Th9) cells and interleukin (IL)-9 are involved in the pathogenesis of several autoimmune diseases. The exact role of IL-9 and Th9 cells in patients with systemic sclerosis (SSc) have not yet been studied adequately. IL-9, IL-9R, transcription factor PU.1 (PU.1), IL-4, thymic stromal lymphopoietin (TSLP) and transforming growth factor (TGF)-β expression were assessed in skin and kidney biopsies of SSc patients and healthy controls (HC) by immunohistochemistry (IHC). The cellular source of IL-9 was also analysed by confocal microscopy analysis. Peripheral IL-9-producing cells were also studied by flow cytometry. The functional relevance of IL-9 increased expression in SSc was also investigated. Our results demonstrated a strong expression of IL-9, IL-9R, IL-4, TSLP and TGF-β in skin tissues of patients with both limited and diffuse SSc. IL-9 expression was observed mainly in the context of skin infiltrating mononuclear cells and keratinizing squamous epithelium. IL-9 over-expression was also observed in renal biopsies of patients with SSc. IL-9 producing cells in the skin were identified as Th9 cells. Similarly, Th9 cells were expanded and were the major source of IL-9 among SSc peripheral blood mononuclear cells (PBMC), their percentage being correlated directly with the modified Rodnan skin score. Infiltrating mononuclear cells, mast cells and neutrophils expressed IL-9R. In in-vitro studies stimulation with rIL-9 significantly induced NET (neutrophil extracellular traps) release by dying cells (NETosis) in neutrophils, expansion of mast cells and increase of anti-systemic scleroderma 70 (Scl70) production by B cells. Our findings suggest that Th9 cells and IL-9 could be implicated in the pathogenesis of SSc.
Agonist antibody and Fas ligand mediate different sensitivity to death in the signaling pathways of Fas and cytoplasmic mutants (1997)

We have produced three forms of human Fas: full-length Fas, Fas with a C-terminal deletion, and a chimera between extracellular Fas and the intracellular domain of the tumor necrosis factor receptor I p55 subunit. We transfected cell lines with these constructs to compare the relative capacity of antibody agonists and the physiological Fas ligand (FasL) to stimulate death. With two agonistic antibodies, the chimera is 100- to 1000-fold more sensitive to induction of death than the full-length Fas. The C-terminal deletion mutant also shows greatly enhanced death in comparison to the wild-type receptor. In contrast, when FasL is used to trigger the Fas pathway, wild-type Fas and the deletion mutant are similarly sensitive, whereas the chimera is 100-fold less susceptible to ligand-mediated killing than Fas. This demonstrates that antibody agonists and natural ligand can stimulate different signaling pathways and emphasizes the limitations of defining physiologically important signaling pathways solely by antibody agonists.
Ligand-independent activation of c-kit receptor tyrosine kinase in a murine mastocytoma cell line P-815 generated by a point mutation (1994)

The c-kit proto-oncogene encodes a receptor tyrosine kinase that is known to play a crucial role in hematopoiesis, especially in mast cell growth and differentiation. Although a number of dominant loss-of-function mutations of c-kit gene have been well characterized in mice, rats, and humans, little is known about the c-kit mutations contributing to ligand-independent activation of the c-kit receptor tyrosine kinase (KIT). In a murine mastocytoma cell line, P-815, KIT has been found to be constitutively phosphorylated on tyrosine and activated in a ligand-independent manner. Sequencing of the whole coding region of c-kit cDNA showed that c-kit cDNA of P-815 cells carries a point mutation in codon 814, resulting in amino acid substitution of Tyr for Asp. Murine wild-type c-kit cDNA and mutant-type c-kit cDNA encoding Tyr in codon 814 were expressed in cells of a human embryonic kidney cell line, 293T. In the transfected cells, mutant-form KITTyr814 was strikingly phosphorylated on tyrosine and activated in immune complex kinase reaction regardless of stimulation with a ligand for KIT (stem cell factor), whereas tyrosine phosphorylation and activation was barely detectable in wild-form KIT. The data presented here provide evidence for a novel activating mutation of c-kit gene that might be involved in neoplastic growth or oncogenesis of some cell types, including mast cells.
Percutaneous electrical nerve stimulation: an alternative to TENS in the management of sciatica (1999)

Sciatica is a common pain problem and current pharmacologic therapies have proven inadequate for many patients. The objective of this sham-controlled investigation was to compare a novel non-pharmacologic technique, percutaneous electrical nerve stimulation (PENS), to transcutaneous electrical nerve stimulation (TENS) in the management of the radicular pain associated with sciatica. Sixty-four consenting patients with sciatica due to lumbar disc herniation were treated with PENS, TENS and sham-PENS according to a randomized, single-blinded, cross-over study. All patients had been maintained on a stable oral non-opioid analgesic regimen for at least 6 weeks prior to entering the study. Each treatment modality was administered for a period of 30 min three times per week for 3 weeks, with 1 week 'off' between each modality. Both PENS and TENS treatments were administered using a stimulation frequency of 4 Hz. The pre-treatment assessment included the health status survey short form (SF-36), as well as visual analog scales (VAS) for radicular pain, physical activity and quality of sleep. The pain VAS was also repeated after each treatment session. At the end of each 3-week treatment block, the SF-36 was repeated. After receiving all three treatment modalities, a global assessment questionnaire was completed. Both PENS (42%) and TENS (23%) were significantly more effective than the sham (8%) treatments in decreasing VAS pain scores. The daily oral analgesic requirements were also significantly reduced compared to the pre-treatment values with PENS (P<0.01) and TENS (P<0.05). However, PENS was significantly more effective than TENS (and sham-PENS) in improving physical activity and quality of sleep. The SF-36 evaluation confirmed the superiority of PENS (versus TENS and sham-PENS) with respect to post-treatment functionality. In the overall assessment, 73% of the patients reported that PENS was the most desirable modality (versus 21% for TENS and 6% for sham-PENS). Finally, 71% of the patients stated that they would be willing to pay extra to receive PENS therapy compared to 22% and 3% for TENS and sham-PENS, respectively. In this sham-controlled study, we concluded that PENS was more effective than TENS when administered at a stimulation frequency of 4 Hz in providing short-term pain relief and improved functionality in patients with sciatica.
Volumetric backprojection (2000)

Article Free Access Share on Volumetric backprojection Authors: Frank Dachille Center for Visual Computing (CVC) and Department of Computer Science, State University of New York at Stony Brook, Stony Brook, NY Center for Visual Computing (CVC) and Department of Computer Science, State University of New York at Stony Brook, Stony Brook, NYView Profile , Klaus Mueller Center for Visual Computing (CVC) and Department of Computer Science, State University of New York at Stony Brook, Stony Brook, NY Center for Visual Computing (CVC) and Department of Computer Science, State University of New York at Stony Brook, Stony Brook, NYView Profile , Arie Kaufman Center for Visual Computing (CVC) and Department of Computer Science, State University of New York at Stony Brook, Stony Brook, NY Center for Visual Computing (CVC) and Department of Computer Science, State University of New York at Stony Brook, Stony Brook, NYView Profile Authors Info & Claims VVS '00: Proceedings of the 2000 IEEE symposium on Volume visualizationOctober 2000 Pages 109–117https://doi.org/10.1145/353888.353907Published:09 October 2000Publication History 3citation559DownloadsMetricsTotal Citations3Total Downloads559Last 12 Months9Last 6 weeks5 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
A Method for Calibrating False-Match Rates in Record Linkage (1995)

Abstract Specifying a record-linkage procedure requires both (1) a method for measuring closeness of agreement between records, typically a scalar weight, and (2) a rule for deciding when to classify records as matches or nonmatches based on the weights. Here we outline a general strategy for the second problem, that is, for accurately estimating false-match rates for each possible cutoff weight. The strategy uses a model where the distribution of observed weights are viewed as a mixture of weights for true matches and weights for false matches. An EM algorithm for fitting mixtures of transformed-normal distributions is used to find posterior modes; associated posterior variability is due to uncertainty about specific normalizing transformations as well as uncertainty in the parameters of the mixture model, the latter being calculated using the SEM algorithm. This mixture-model calibration method is shown to perform well in an applied setting with census data. Further, a simulation experiment reveals that, across a wide variety of settings not satisfying the model's assumptions, the procedure is slightly conservative on average in the sense of overstating false-match rates, and the onesided confidence coverage (i.e., the proportion of times that these interval estimates cover or overstate the actual false-match rate) is very close to the nominal rate.
Pancreatic Islet Cells in Preobese Yellow Avy/- Mice: Relation to Adult Hyperinsulinemia and Obesity (1994)

Plasma insulin levels in yellow Avy/- mice begin to increase before the animals are overtly obese. Are the elevated insulin levels in yellow mice primary or secondary to the subsequent obesity? Elevated blood insulin levels in young preobese mice, due to synthesis and release of insulin by increased number of beta cells, would stimulate lipogenesis, resulting in excess lipid deposition and subsequent peripheral insulin resistance. Examination of this possibility was the objective of this study. The beta, alpha, and delta cells in the pancreata of 7-, 14-, and 21-day-old male yellow Avy/A and agouti A/a (BALB/c x VY)F1 hybrid mice were counted with immunohistochemical/morphometric techniques. The insulin and glucagon concentrations in pancreata from male and female mice of the same ages and genotypes were also assayed. In the 21-day-old male mice, the mean number of beta cells/pancreas was significantly greater in the yellow mice than in the agouti mice; however, insulin content and body weight were the same. This suggests that increased beta cell proliferation in yellow mice precedes any detectable genotype-specific increase in pancreatic insulin content or body weight.
Neuropathic pain associated with non-surgical treatment of breast cancer (2005)

Jung, Beth F.a; Herrmann, Davidb; Griggs, Jenniferc,d; Oaklander, Anne Louisee; Dworkin, Robert H.a,b,* Author Information
Pathogenesis ofStaphylococcus aureusnecrotizing pneumonia: the role of PVL and an influenza coinfection (2013)

Only recently necrotizing pneumonia was defined as a specific disease entity that is caused by a Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strain and is frequently preceded by an influenza infection. Necrotizing pneumonia is characterized by a sudden onset and rapid worsening of symptoms, leukopenia, airway hemorrhages, severe respiratory failure and a high mortality rate. Despite clear epidemiological data, the function of PVL in necrotizing pneumonia has been controversially discussed due to conflicting results from different disease models. Furthermore, there are many proposed mechanisms how a viral infection could facilitate and interact with a bacterial superinfection. In this review, we summarize current data from 43 clinical cases and results from various infection models on necrotizing pneumonia. We discuss the contribution of S. aureus PVL and a preceding influenza infection and present a concept of the pathogenesis of necrotizing pneumonia.
Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection Method (2014)

In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.