gebhart/patrepeval huggingface dataset:
Therapeutic Implications of Interleukin-10 in Inflammatory Bowel Disease. (1998)

Enhanced production of proinflammatory cytokines has been described in inflammatory bowel disease. The effect of interleukin (IL)-10, a cytokine with antiinflammatory activity, or anti-IL-10, on cytokine production by cultured colonic mucosa or blood mononuclear cells from patients with active inflammatory bowel disease was evaluated. Addition of IL-10 to the culture medium of colonic tissues or blood mononuclear cells resulted in inhibition of both IL-1 beta and tumor necrosis factor-alpha production and augmentation of IL-1 receptor antagonist production. Conversely, neutralization of endogenous IL-10 was found to augment both tumor necrosis factor-alpha and IL-1 beta production and inhibit IL-1 receptor antagonist production. In addition, the production of IL-10 by mononuclear cells was suppressed by prednisolone. In conclusion, IL-10 and related molecules may be useful in the treatment of inflammatory bowel disease.
A new pitfall in plasma amino acid analysis. (1989)

Journal Article A new pitfall in plasma amino acid analysis. Get access P Parvy, P Parvy Lab. Biochim. Med. B, Hôpital Necker-Enfants Malades, Paris, France Search for other works by this author on: Oxford Academic Google Scholar J Bardet, J Bardet Lab. Biochim. Med. B, Hôpital Necker-Enfants Malades, Paris, France Search for other works by this author on: Oxford Academic Google Scholar D Rabier, D Rabier Lab. Biochim. Med. B, Hôpital Necker-Enfants Malades, Paris, France Search for other works by this author on: Oxford Academic Google Scholar J P Bonnefont, J P Bonnefont Lab. Biochim. Med. B, Hôpital Necker-Enfants Malades, Paris, France Search for other works by this author on: Oxford Academic Google Scholar P Kamoun P Kamoun Lab. Biochim. Med. B, Hôpital Necker-Enfants Malades, Paris, France Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 35, Issue 1, 1 January 1989, Page 178, https://doi.org/10.1093/clinchem/35.1.178 Published: 01 January 1989
Cloning and characterization of cyclophilin C-associated protein: a candidate natural cellular ligand for cyclophilin C. (1993)

We report the protein purification and the cloning and characterization of a cDNA encoding the proteins that bind with high affinity to cyclophilin C (Cyp-C) in the absence of cyclosporin A. Transfection of this cDNA into COS cells directs the production of a glycoprotein of 77 kDa that binds to Cyp-C in the absence, but not the presence, of cyclosporin A. Homology comparisons reveal that this protein and gene, termed CyCAP for Cyp-C-associated protein, possess a cysteine-rich domain (scavenger receptor cysteine-rich domain) found in a variety of cell-surface molecules; the rest of the sequence is apparently specific. This result raises the possibility that Cyp-C serves as a mediator or regulator of an as-yet-unidentified signal or cellular process initiated via the Cyp-C-associated protein.
Perfluoro-cis-2,3-Dialkyloxaziridines: Effective Reagents for the Selective Oxidation of Ethers to Carbonyl Compounds (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPerfluoro-cis-2,3-Dialkyloxaziridines: Effective Reagents for the Selective Oxidation of Ethers to Carbonyl CompoundsAlberto Arnone, Rosanna Bernardi, Marcello Cavicchioli, and Giuseppe ResnatiCite this: J. Org. Chem. 1995, 60, 8, 2314–2315Publication Date (Print):April 1, 1995Publication History Published online1 May 2002Published inissue 1 April 1995https://pubs.acs.org/doi/10.1021/jo00113a002https://doi.org/10.1021/jo00113a002research-articleACS PublicationsRequest reuse permissionsArticle Views236Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Reduction of Hyperinsulinemia and Insulin Resistance by Opiate Receptor Blockade in the Polycystic Ovary Syndrome with Acanthosis Nigricans* (1987)

We previously reported that circulating beta-endorphin levels are increased in obese hirsute women and that plasma immunoreactive insulin (IRI) levels are increased in proportion to the degree of hyperandrogenism in women with the polycystic ovary (PCO) syndrome. We, therefore, tested the hypothesis that endogenous opiates are at least partially responsible for the hyperinsulinemia and insulin resistance in this syndrome. In the first study, acute naloxone administration significantly reduced the plasma IRI response and IRI/glucose ratio in three euglycemic obese women with PCO and acanthosis nigricans (AN) and marked insulin resistance, but did not alter the glucose response. Naloxone had no effect on these parameters in the normal weight control subjects. In the second study, nalmefene, a new, orally active opiate antagonist, reduced IRI and the IRI/glucose ratio in four women with PCO-AN and marked hyperinsulinemia in a randomized, double blind, crossover protocol. We conclude that endogenous opiates are at least partially responsible for the hyperinsulinemia and insulin resistance in PCO-AN.
Microencapsulated dog and rat islet xenografts into streptozotocin-diabetic and NOD mice. (1990)

Microencapsulation is an appealing method for islet xenografting. However, graft failure has been related to a cellular reaction, particularly intense in spontaneously diabetic, NOD mice. The goal of this study was to characterize this reaction. Poly-l-lysine-alginate microcapsules containing dog or rat islets were xenografted intraperitoneally into streptozotocin (SZN)-diabetic C57BL/6J and spontaneously diabetic NOD mice, with or without recipient treatment with GK 1.5 (anti-CD4 monoclonal antibody). Both dog and rat islets in microcapsules normalized blood glucose (BG) routinely in both SZN and NOD mice within 24 hours. Empty microcapsules and GK 1.5 treatment did not affect BG. NODs destroyed both microencapsulated dog and rat islets more rapidly than did SZN-diabetic mice (P less than 0.01). Graft biopsies showed an intense cellular reaction and no viable islets. GK 1.5 treatment significantly prolonged both dog-to-NOD and rat-to-NOD grafts (P less than 0.01). Biopsies of long-term functioning grafts (on days #70-#95) demonstrated viable islets and no cellular reaction. In prediabetic NODs, encapsulated dog and rat islets elicited a moderate cellular reaction. Empty microcapsules excited no cellular reaction in either diabetic or prediabetic NODs.
Effect of passivation and aging on the photoluminescence of silicon nanocrystals (2001)

Silicon nanocrystals were prepared by laser pyrolysis of silane in a gas flow reactor and deposited on substrates by cluster beam deposition. The evolution of the photoluminescence was measured as a function of the time the samples were exposed to air. With gradual air exposure and oxidation, the photoluminescence increases in intensity and its peak wavelength shifts to the blue. At the same time, the photoluminescence band becomes wider. To remove the oxide layer, the samples were exposed to hydrofluoric acid (HF) vapor. The HF attack has no influence on the band position but leads to a smaller width. The observations can be explained in terms of quantum confinement as the origin of the photoluminescence. Only for the smallest Si nanocrystals, an interface state seems to limit the maximum photoluminescence energy to 2.1 eV.
Immune and Antibody Responses to an Isolated Capsid Protein of Foot-and-Mouth Disease Virus (1975)

The purified capsid proteins VP1, VP2, and VP3 of foot-and-mouth disease virus type A12 strain 119 emulsified with incomplete Freund's adjuvant were studied in swine and guinea pigs. Swine inoculated on days 0, 28, and 60 with 100-mug doses of VP3 were protected by day 82 against exposure to infected swine. Serums from animals inoculated with VP3 contained viral precipitating and neutralizing antibodies, but such serums recognized fewer viral antigenic determinants than did antiviral serums. Capsid proteins VP1 and VP2 did not produce detectable antiviral antibody in guinea pigs, and antiviral antibody responses in swine to a mixture of VP1, VP2, and VP3 were lower than the responses to VP3 alone. However, when swine were inoculated with VP1, VP2, and VP3 separately at different body sites, no interference with the response to VP3 was observed. Vaccine containing VP3 isolated from acetylethylenimine-treated virus appeared less protective for swine than vaccine containing VP3 from nontreated virus. Trypsinized virus, which contains the cleaved peptides VP3a and VP3b rather than intact VP3, produced approximately the same levels of antiviral antibody responses in guinea pigs as did virus. Conversely, an isolated mixture of VP3a and VP3b did not produce detectable antiviral antibody responses in guinea pigs. The VP3a-VP3b mixture did, however, sensitize guinea pigs to elicit such responses following reinoculation with a marginally effective dose of trypsinized virus.
Serial Changes in Serum VEGF and HGF in Patients with Acute Myocardial Infarction (2000)

The time course of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) release in patients with acute myocardial infarction (AMI) is unknown. Blood samples were obtained at the time of admission and 3, 7, 14 and 21 days later in 32 patients with AMI and 30 control patients. Serum VEGF and HGF, as well as C-reactive protein (CRP) and amyloid A protein (SAA), were determined. Both serum VEGF and HGF levels on admission in patients with AMI were higher than control values and peaked on day 7. VEGF levels in patients with preinfarction angina were higher than in patients with no preinfarction angina, whereas the HGF level did not differ. Both CRP and SAA levels peaked on day 3, and the CRP level on day 3 correlate with both VEGF and HGF levels on day 7. We hypothesized that the serum VEGF level is associated with preinfarction ischemia and the increase in VEGF and HGF on day 7 of AMI may represent a response to acute inflammation.
P2‐323: Memapsin 2 (beta‐secretase, BACE) immunization as specific and safe therapy for Alzheimer's disease (2008)

We developed an active immunization strategy that elicits neutralizing antibodies to memapsin 2 (BACE, beta-secretase, M2) that inhibit Abeta production as a potential therapy for Alzheimer's disease (AD) (FASEB J. 21, 3184–3196, 2007). Such an immunotherapy can be improved by understanding the mechanism of Abeta inhibition by anti-M2 antibodies using both animal and cellular models. The results demonstrated that intravenously injected anti-M2 antibodies cross the blood-brain barrier and bind to M2-expressing neurons in the AD mouse model (Tg2576). In M2-transfected cells, anti-M2 antibodies colocalized on the cell surface with substrate amyloid precursor protein (APP) and endocytosed together with M2 to reside in the early endosomes. Memapsin 2 activity is inhibited by antibodies masking active site access and blocking production of Abeta. In younger Tg2576 mice, 12-months of M2-immunization provided approximately 40% and 20% reduction on brain Abeta levels and amyloid plaque deposits, respectively. To determine whether M2 immunization is safe, we analyzed for T cell, microglia, and astrocyte infiltration by immunohistochemistry in the brain as indicators of a potential inflammatory autoimmune response, and performed analysis of antibody isotype by ELISA. Minimal epitopes of M2 were tested for immunoreactivity with polyclonal anti-M2 antibodies using 203 overlapping octa-peptides of the M2 ecto-domain by epitope mapping ELISA. No obvious differences in T cell/microglia/astrocyte staining between the brains of immunized and control mice were seen. The antibody isotypes raised by M2-immunization were shown to be largely IgG1 and IgG2b which represent the noninflammatory Th2 immunoglobulins. Results from epitope mapping revealed that several specific epitopes interacted with antibodies with high binding affinity. The ×-ray crystal structure of M2 revealed that the highly antigenic epitopes are exposed on surface loops and secondary structures of M2 around the active-site cleft (and the flap covering the active site) and the APP interaction site. Results indicate that M2 (beta-secretase) immunization is a safe immunotherapy which will not trigger any unwanted autoimmune or inflammatory responses. We also identified the minimal effective M2 epitopes for immunization. We are currently investigating the efficacy of antibodies specific to these potentially effective M2 epitopes to block Abeta production.
Advances in on-chip photodetection for applications in miniaturized genetic analysis systems (2003)

Microfabrication techniques have become increasingly popular in the development of next generation DNA analysis devices. Improved on-chip fluorescence detection systems may have applications in developing portable hand-held instruments for point-of-care diagnostics. Miniaturization of fluorescence detection involves construction of ultra-sensitive photodetectors that can be integrated onto a fluidic platform combined with the appropriate optical emission filters. We have previously demonstrated integration PIN photodiodes onto a microfabricated electrophoresis channel for separation and detection of DNA fragments. In this work, we present an improved detector structure that uses a PINN + photodiode with an on-chip interference filter and a robust liquid barrier layer. This new design yields high sensitivity (detection limit of 0.9 ng l -1 of DNA), low-noise (S/N 100/1) and enhanced quantum efficiencies (>80%) over the entire visible spectrum. Applications of these photodiodes in various areas of DNA analysis such as microreactions (PCR), separations (electrophoresis) and microfluidics (drop sensing) are presented.
Robust vision-based localization for mobile robots using an image retrieval system based on invariant features (2003)

We present a vision-based approach to mobile robot localization, that integrates an image retrieval system with Monte-Carlo localization. The image retrieval process is based on features that are invariant with respect to image translations, rotations, and limited scale. Using the local features the system is robust against distortion and occlusions, which is especially important in populated environments. By using the sample-based Monte-Carlo localization technique our robot is able to globally localize itself to reliably keep tracking of its position, and to recover from localization failures. Both techniques are combined by extracting for each image a set of possible view-points using a two-dimensional map of the environment. Our technique was implemented and tested extensively. We present several experiments demonstrating the reliability and robustness of our approach even in the context of dynamics in the environment and larger errors in the odometry.
γ9 and δ2CDR3 domains regulate functional avidity of T cells harboring γ9δ2TCRs (2012)

Immunotherapy with innate immune cells has recently evoked broad interest as a novel treatment option for cancer patients. γ9δ2T cells in particular are emerging as an innate cell population with high frequency and strong antitumor reactivity, which makes them and their receptors promising candidates for immune interventions. However, clinical trials have so far reported only limited tumor control by adoptively transferred γ9δ2T cells. As a potential explanation for this lack of efficacy, we found unexpectedly high variability in tumor recognition within the physiologic human γ9δ2T-cell repertoire, which is substantially regulated by the CDR3 domains of individual γ9δ2TCRs. In the present study, we demonstrate that the reported molecular requirements of CDR3 domains to interact with target cells shape the physiologic γ9δ2T-cell repertoire and, most likely, limit the protective and therapeutic antitumor efficacy of γ9δ2T cells. Based on these findings, we propose combinatorial-γδTCR-chain exchange as an efficient method for designing high-affinity γ9δ2TCRs that mediate improved antitumor responses when expressed in αβT cells both in vitro and in vivo in a humanized mouse model.
Magical mystery tour: MLO proteins in plant immunity and beyond (2014)

Stable heritable restriction of the ubiquitous powdery mildew disease is a desirable trait for agri and horticulture. In barley (Hordeum vulgare), loss-of-function mutant alleles of the Mildew resistance locus o (Mlo) gene confer broad-spectrum resistance to almost all known isolates of the fungal barley powdery mildew pathogen, Blumeria graminis f.sp. hordei. Despite extensive cultivation of barley mlo genotypes, mlo resistance has been durable in the field. Mlo genes are present as small families in the genomes of all higher plant species. The presumed negative regulatory role of particular members in plant immunity is evolutionarily conserved, as powdery mildew resistant mlo mutants have also been described in Arabidopsis thaliana, tomato(Solanum lycopersicum) and pea (Pisum sativum). Barley Mlo encodes a plasma membrane-localized seven-transmembrane domain protein of unknown biochemical activity. Here, we review the known requirements for mlo-mediated disease resistance in barley and Arabidopsis and reflect current views regarding Mlo function. We discuss additional mlo mutant phenotypes recently discovered in Arabidopsis and present a meta-analysis of the phylogenetic relationships within the Mlo family. Finally, we consider the novel versatile tools for functional analysis and targeted genome modification that can be used to induce mlo-based powdery mildew resistance in virtually any plant species.
Vulnerability Impact Analysis Framework for Network Security (2007)

Monitoring and quantifying component behavior is key to make network systems security and robust. This paper proposes an online monitoring and analysis framework based on agents for monitoring, analyzing and quantifying network vulnerability in real time. It proposes an algorithm for valuation of network components to populate an asset list with ”criticality” values for impact analysis, and presents impact factors calculating methods to quantify how attacks and faults impact network performance and services. System administrators can use the framework to analyze and proactively manage the effects of complex network faults and attacks and recover accordingly.
Peptide permeases from Streptococcus pneumoniae affect adherence to eucaryotic cells (1995)

To gain access to tissues within the human host, Streptococcus pneumoniae initially colonizes the nasopharynx and then interacts with glycoconjugates on the surfaces of target cells at various sites of infection. Although pneumococcal adhesins are currently unknown, exported proteins on the bacterial surface are potential candidates. To identify bacterial elements involved in this process, mutants of S. pneumoniae with defects in exported proteins were screened for the inability to adhere to cells representative of three in vivo niches: (i) agglutination of bovine erythrocytes, which reflects adherence to cells which reside in the nasopharynx; (ii) human type II pneumocytes (lung cells [LC]), representing the alveolar site of infection; and (iii) human vascular endothelial cells (EC), representing the endovascular site. The capacity of the mutants to adhere during the course of pneumococcal disease was also assessed by using cytokine-activated LC and EC. All of the 30 mutants analyzed produced hemagglutination values comparable with those of the parent strain. Four independent mutants demonstrated a greater than 50% decrease in adherence to both LC and EC. Sequence analysis of the altered alleles from these strains showed that mutations had occurred in two previously identified loci, plpA and ami, which belong to the family of genes encoding protein-dependent peptide permeases. Mutations in the ami locus resulted in an inability to recognize the GalNAc beta 1-4Gal glycoconjugate receptor present on resting LC and EC, whereas mutations in plpA resulted in a failure to recognize a GalNAc beta 1-3Gal glycoconjugate receptor also present on resting cells. Mutations in neither allele affected recognition of GlcNAc receptors present on cytokine-activated LC and EC. These results suggest that peptide permeases modulate pneumococcal adherence to epithelial and endothelial cells either by acting directly as adhesins or by modulating the expression of adhesins on the pneumococcal surface during the initial stages of colonization of the lung or the vascular endothelium.
Controlling Emitting Dipole Orientation with Methyl Substituents on Main Ligand of Iridium Complexes for Highly Efficient Phosphorescent Organic Light‐Emitting Diodes (2015)

Orientation of emission dipoles of heteroleptic iridium complexes in an emitting layer is controlled toward the horizontal direction by substituting the hydrogen atoms of the cyclometalated ligand by methyl groups and demonstrates an organic light-emitting diode with an unprecedentedly high external quantum efficiency of 34.1% and power efficiency of 157.6 lm W−1 using a new green emitter. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The Application of DRCS Within the North American Broadcast Teletext Specification (1982)

The North American Broadcast Teletext Specification makes uses of the AT&T Presentation Level Protocol (PLP) for the coding of text and graphic information. It has been suggested that one method for achieving relatively high resolution graphics without burdening user terminal cost is through DRCS. While this is a valid method technically, it presents many problems which relate to access time, transmission efficiency, terminal design and display quality. This paper will detail some of the problems in implementing DRCS as an alternate method for the coding and display of higher resolution graphics within the framework of PLP.